LOCUS       DASWZT010000001         4680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_38737, whole genome shotgun sequence.
ACCESSION   DASWZT010000001 DASWZT010000000
VERSION     DASWZT010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38737"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..1351
                     /locus_tag="VGE92_00005"
     CDS             65..1351
                     /locus_tag="VGE92_00005"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HEY1312223.1"
                     /translation="MSELGAQRSRKDLQCTADAMSYQRRYAAELKRRVVEQGDPFVVA
                     QADTPHEIFHAMDIALITNQWWSAYISAKQLSGPYFKVLQQHGYPGNGCRYCSLGLAC
                     TLANDPASAPWGGLPRPVALVARLTCDCIQQVFGLWAAALKTEFFALEAPAWTHKDAA
                     WFEKSNDRWEEVFQPDRIELLVQEMRALIAMLESKTGRQFSEAKLTVLMEKINEQEGY
                     IAEAARMIGAARPCPVSIADQMPNTMIPQWHRGSDWAVAHAKRFRDEVAERVRAGTGV
                     SSNERIRLMWIGAGLWHDPGFYNALEERLGAVFVWSMYMPFAGPQYIRKMHGKPLHAL
                     ASRICSMNEVLHLPPWMNGWMVSEAKRCGIDAAVMLVPPDSRLSQSGTKLTQLSLEQA
                     GVPTLALDANMVDAQGWSHEQMVNHVSDFLQGARLA"
     gene            1348..2433
                     /gene="dctP"
                     /locus_tag="VGE92_00010"
     CDS             1348..2433
                     /gene="dctP"
                     /locus_tag="VGE92_00010"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEY1312224.1"
                     /translation="MKSWLAGVLLFAVAVAVAVAAPGAESSPAVVSYPTVLSYATPYG
                     PNHTFSRADKTWMQWVAAHSGGRLQIRPIWSGGLISSDQSLIELRHGVADIGLITPIY
                     ARGGAHLIRVQTGFYAGAKTYEQQVAMYRCLIAAAPEYAEELTGLKILAVQGGTLPGL
                     LVRTREVHTLEDLRGLRIRVPTELLNVMRDLGADPVTMPMGEVYSSLAKGVLDAVVAP
                     ADTLKSMHFGEVAKYYTELEVPRGAYPSRAMGDKRWRSLTPEERAILEASIPVWEAAL
                     STETQAAARSGEIEGRAQGVRFIPIGAAEQRRFDDLYEEDAGRAAQSLSRFDIDGTAV
                     FNEARQIAKGLEQTGQVVCPRGENAAT"
     gene            2420..3619
                     /locus_tag="VGE92_00015"
     CDS             2420..3619
                     /locus_tag="VGE92_00015"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY1312225.1"
                     /translation="MQPLEGIRIADFSHIMAGPYATHLLRLLGAEVIKIEAPVRGDGF
                     RTYGMDRTYDGMAPAFIAVNAGKKSVSLDLKDPASLEAARRLIARSDVLVENFRPGVM
                     SRLGLGYPQVRSLKGDIVYCSISGYGQSGPLRDWPAIDNIVQATSGMMSLGGEPGDPP
                     VRVGFPVVDTLTGQTAAFAILAALFRRQRNGQGEYIDVAMMDASLAFMASAVVPFLVT
                     GQVLERTGNTGYSGQPTAALFEAADGKLISLGVVQQGQFEALARLLGRAEWLTDARYA
                     TADLRRANAAPLKASLAEILRQQNAAEWEARMSAAGIPCGMVRGVADAASLPHLEQRN
                     LKLPLRIPSLGAKGHVAVMNAGFMFNDGGPGVAEPPPVLGGHTEEVLRWLDFDEAQRR
                     AIMGKGE"
     gene            3621..>4680
                     /locus_tag="VGE92_00020"
     CDS             3621..>4680
                     /locus_tag="VGE92_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY1312226.1"
                     /translation="MLKALRTLVAACVCFTAAAHAAPGESAFGVYHGYSAAAFPDQIK
                     SSFYLPMRDGVRLAVDLYRPGANGKAAEGRFPVVWHHNFDRRYVAAPEANAEYAAPLL
                     TQYGYVVAVVERRGLGASFGERRGYNDRIEAEDAYEVNEWLARQSWSTGKVGIVGCSN
                     TGAAAMHAITIRPPHLLAAFVGCFAWDTYDCTLRGGIFAQWGTGVQRTVEEDMKAKPV
                     DGDESKTLLAQAAQEHQKSTNLAAMWRGLPYRDDFSPLTASRFWNEGSIASYADEMRQ
                     SGVVLYITGGWRDDLRKDGLITFSNWTPGKRHIVIGPWTHCRNEGFDLFAEMHRFFDF
                     YLQGIDNGFANDPPIHYFT"
BASE COUNT          853 a         1377 c         1579 g          871 t
ORIGIN      
        1 cgcagacgct gtgtggcctg agcggcgcgc gcgttgccga gttgcaatct ctgggagtgt
       61 ttcgatgagc gagttgggcg cacaaagaag tcgcaaggat ttgcaatgca ccgccgatgc
      121 gatgagctat cagcgccgtt atgcggccga actcaaacgg cgcgtcgtcg agcagggcga
      181 tcccttcgtg gtagcgcagg ccgatacgcc gcacgagatc ttccacgcca tggatatcgc
      241 actgataacc aatcaatggt ggtcggcgta catctccgcc aagcaactgt cggggcccta
      301 tttcaaggtg ttgcagcagc atggctaccc gggcaatggc tgccgctact gttccctggg
      361 tctggcctgc acgctcgcga acgatccggc cagcgcgccg tggggcggat tgccgcggcc
      421 ggtcgcgctg gtggcgcggc tcacctgcga ctgcatccaa caggtctttg gtctgtgggc
      481 agccgccctg aagaccgagt tcttcgcgct cgaggcgccg gcatggacgc acaaagacgc
      541 ggcatggttc gaaaaatcga acgatcgctg ggaggaggtt tttcaacccg accgcatcga
      601 acttctggtg caggaaatgc gtgcgctgat cgcgatgctg gagtcgaaga ccgggcggca
      661 gttcagtgaa gcgaagctca cggtattgat ggagaagatc aacgaacagg aaggctatat
      721 cgccgaggcg gcgcgcatga tcggtgcggc gcgcccctgc ccggtgtcga ttgcggatca
      781 gatgccgaac accatgatcc cgcaatggca ccggggttcg gactgggcgg tcgcgcatgc
      841 gaagcgtttt cgcgatgaag tggcggagcg ggttcgggcg ggcaccggcg tgagcagcaa
      901 cgagcgcatt cgtttgatgt ggatcggagc gggactgtgg cacgacccgg gcttctacaa
      961 tgcgctggaa gagcgtctgg gcgccgtgtt cgtttggtcg atgtacatgc cgttcgccgg
     1021 cccgcagtac atcagaaaga tgcacggcaa gcctctgcac gcactcgcga gccgcatctg
     1081 ctcgatgaac gaggtgctgc atttgccgcc gtggatgaac ggctggatgg tgagcgaagc
     1141 caagcgctgc gggatcgatg ccgccgtcat gctggtgccg cccgacagcc gtctgtcgca
     1201 gagcggcacg aagctgactc agctgtcgct cgagcaggcg ggtgtgccca cgctggcttt
     1261 ggacgccaac atggtggatg cgcagggctg gagtcatgaa cagatggtca atcacgtctc
     1321 ggactttctg caaggcgcga ggctcgcatg aaaagttggc tcgccggtgt tctattgttc
     1381 gcggtcgcgg tcgcggtcgc ggtcgcggcg ccgggcgctg aatcgagccc ggccgttgtg
     1441 agctacccca ccgttctcag ctacgcgaca ccgtacgggc ccaaccacac gttcagccgc
     1501 gcggacaaaa cgtggatgca atgggttgcc gcgcactccg gcggccgttt gcaaattcgg
     1561 cccatctggt cgggcggtct gatttcatcg gatcaatcct tgatcgagct gcgtcacggc
     1621 gtggccgaca tcggtctcat caccccgatt tatgcgcgtg gcggagccca tctcatccgc
     1681 gtgcagacgg gcttttatgc cggggccaag acttacgaac agcaggtggc catgtaccgg
     1741 tgcctgatcg ccgcggcgcc cgaatacgcc gaggaattga cggggctgaa gattctcgcc
     1801 gttcagggcg ggacgttgcc ggggctcttg gtgcgcaccc gcgaagttca cactctggag
     1861 gatttgcgcg gacttcgaat acgggtgccg acggagcttt tgaacgtgat gcgcgatctc
     1921 ggcgcggatc ccgtgaccat gcccatgggc gaggtgtatt ccagcctggc gaaaggagtt
     1981 ctcgatgccg tcgtcgcgcc ggcggatacg ctcaagtcga tgcatttcgg cgaggtcgcc
     2041 aagtattaca ccgaactcga ggtgccgcgg ggtgcctatc cttcccgcgc gatgggcgac
     2101 aagcgctggc ggtccctgac ccccgaggaa cgagcgatat tggaggcctc catcccagta
     2161 tgggaggccg ctctctcgac ggagacccag gcggccgccc gatcgggcga gattgaaggt
     2221 cgcgcacaag gcgtgcggtt cattccgatc ggcgcggcag agcagcggcg cttcgatgat
     2281 ctttatgaag aggatgcggg gcgggccgcg caatcactgt cgcgattcga catcgacgga
     2341 acggcggtgt tcaacgaggc gcggcagatt gccaaagggc tcgaacagac cggtcaggtc
     2401 gtgtgcccga ggggtgaaaa tgcagccact tgaaggaatc agaatcgccg acttcagcca
     2461 tatcatggcg ggtccctatg caacgcattt actgcgtctg ctcggcgccg aggtaatcaa
     2521 gatcgaagcg cccgtgcgcg gcgacggttt tcgcacttac ggcatggatc gaacttacga
     2581 cggcatggcg ccggccttca ttgcggtgaa tgccggcaag aaatcggtat ccctggacct
     2641 caaagatccg gcctcgctgg aggcggcacg ccgcttgatc gcgcgctcgg atgtgctggt
     2701 ggaaaatttc cgccccgggg tgatgtcgcg cctcggtctg ggctacccgc aggtacgaag
     2761 cctgaagggg gacatcgttt attgttccat ctccggttac ggccagtcgg ggccgctgcg
     2821 ggattggccg gcgatcgata acatcgtgca ggccaccagc gggatgatgt ccttgggcgg
     2881 tgaacccggc gatccacctg tacgcgtggg attcccggtg gtcgatacgc tgaccggaca
     2941 gacggcggcg ttcgccattc tcgcggcgct gtttcggcgg cagcgcaacg gccagggtga
     3001 atacatcgac gttgccatga tggatgcgag ccttgcgttc atggcctcgg ccgtggtgcc
     3061 ttttctcgtg accggacagg tattggagcg taccgggaat accggataca gcgggcagcc
     3121 gactgcggca ctgttcgaag cggcggatgg caagctcatt tccttaggcg tggtgcagca
     3181 ggggcaattc gaggcgctgg cgcggctgct gggccgagcc gaatggctga ccgatgcgcg
     3241 ctatgcaacg gcggacttgc ggcgcgccaa cgcggcgcca ctcaaggcct cgttggccga
     3301 gattctccgg cagcaaaacg cagcggaatg ggaggccagg atgagcgctg ccggaatccc
     3361 ctgtggaatg gttcgcggcg tcgccgacgc cgcatcgcta ccgcatctcg agcagcgaaa
     3421 cctcaaattg ccgctgcgca taccaagtct cggggccaag ggacatgtcg cagtcatgaa
     3481 cgcgggtttc atgttcaacg acggcggtcc gggtgttgcc gaaccgccgc ccgtgctcgg
     3541 cggccacacc gaggaggtgc tgcgatggct cgacttcgat gaggcgcaac ggcgggcaat
     3601 catgggcaag ggggagtgac atgttgaaag cgttacgcac cctggtggcg gcctgtgtgt
     3661 gcttcacggc ggccgcgcac gccgcgcccg gcgagtcggc gttcggtgtc taccatggat
     3721 actccgccgc ggcgttcccc gaccagatca agagttcctt ttatctgccg atgcgcgacg
     3781 gcgtgcggct ggcggtggat ctgtatcgcc ccggcgcgaa tggcaaggca gccgagggcc
     3841 gctttccggt ggtgtggcat cataacttcg accgtcgcta cgtggcggcc cctgaagcca
     3901 atgccgagta tgccgctccg ctgttgacgc aatacggtta cgtcgtggcc gtggtcgagc
     3961 gccgcgggct gggcgcatcc ttcggtgagc ggcgcggcta taacgatcgc atcgaggcgg
     4021 aagacgcgta cgaagtcaac gaatggctgg cgcgtcagtc ctggtcgacg ggcaaggtgg
     4081 gcatcgtcgg ctgttcaaat accggcgccg cggccatgca cgccatcacc atccgcccgc
     4141 cgcatctgtt ggcggcattc gtcggctgct tcgcttggga tacctacgac tgcaccttgc
     4201 gcggcgggat tttcgcgcag tggggaaccg gcgtgcagcg aaccgtcgag gaggacatga
     4261 aagccaaacc ggtagacggc gatgaatcca aaactctgct ggcgcaagcg gcgcaggaac
     4321 atcagaagag cacgaacctc gcggcgatgt ggcgggggct accgtaccgc gatgatttca
     4381 gtccgctgac ggccagccga ttttggaacg agggcagcat tgcctcctat gccgatgaga
     4441 tgcggcagtc gggagtcgtg ctttacatca ccggcggctg gcgcgacgac ctgcgcaagg
     4501 atggcttgat caccttcagc aattggaccc cgggcaagcg tcacatcgtc atcggaccgt
     4561 ggacgcattg ccgcaacgaa ggcttcgacc tgttcgccga aatgcatcgc ttcttcgact
     4621 tctatttgca aggcatcgac aacggattcg ccaacgaccc gccgatccat tatttcaccg
//
LOCUS       DASWZT010000002         6306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_87160, whole genome shotgun sequence.
ACCESSION   DASWZT010000002 DASWZT010000000
VERSION     DASWZT010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6306
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87160"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2947
                     /locus_tag="VGE92_00025"
     CDS             <1..2947
                     /locus_tag="VGE92_00025"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY1312227.1"
                     /translation="SRADRWGFDNQVYGWTPDGTAILFRSARDGFTLTDSKLYTVPMN
                     GGAASALPMPVSGAGYFSPDGKKVVYSPLWRDFRSEKRYQGGWANDLFIFDLAHPSLL
                     QVTNDPRTDRDPMWIGDAIYFNSDRTGVFNLYRYDIASRQTRQLTHSTDWDVRWPSAD
                     AEGQIVYESDGELHLYDTRTDQDRALSITVPADATTTRPQTVNAADNIESMSVSPGGE
                     RLAMVARGDVFSVPVEHGITRNLTQSSSAHDREAAWSSDGKRLAYVSDRSGEEEIYVQ
                     DQDGSTPAVQVTSGSSARYFAPRWSGDNRRIAFCDHTGSLFVVEVATQRRNLVAHDPA
                     DLSQDYAWSPDGQFLGFSLNDANGFSSVEIWSAADGKMRRVTPQSFNAHSPAWAPHGE
                     LLYFLSEREYQPLLSTIEFDFATDRQTGIFALTLRKDGKNPYGIRDDEPGDEKKDADK
                     EEPGKDKKKPPAPVRIDFAGIEQRAIRVPLEPDNLSNLQVGEDSLLYQRSGPIYYGRD
                     SSVKASVMAYSIKDREAKPVVEDVTEWSATADGKHVLAQLSSKEIKYFEVGKSDDAKP
                     VSLAGLITTRVPAEEWGEIFADVWRRYRDYFYVANMHGYDWQKLREKYQPLVGIVGSR
                     ADLNYVIAEMISELTVQHAYIEGGDLGLPKRPFVALPGARFELDARAGRYRIAKIFAG
                     ENEEEHYRSPLTEVGVDVHVGDYLLAINGRELKAGTDPYELLRAAPNQPVEWRVSASA
                     DGRAARSIRYQPLASETNLLYLAWVSENRARVDRLSQGRLGYIHIPDMGEAGIREFIK
                     WWYPQVRKEGLVVDVRDNGGGNISELLIERLARTLHGTSFARNHQTTDTYPRVVQPGP
                     KVALINEGTASDGDIFSHEFRQWKIGPLIGKRTWGGVVGITDHGPLLDGGMVFVPEFG
                     TADENGHWIIEGHGVDPDIVIEQDPVEVLKGRDPQLERGVAELLQRLPRTPRGLPQRD
                     APPIKTGAP"
     gene            2950..3414
                     /locus_tag="VGE92_00030"
     CDS             2950..3414
                     /locus_tag="VGE92_00030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1312228.1"
                     /translation="MSELSTLGLACALDAREIAEMSRDLIEHGLTWSWTPARVQHFIC
                     GPESSVVVARRERHIAAFAIMHFGDEVAHLNLLAVAPEHRRQGLGRQLMSWLTETAII
                     AGVFRINLELRTHNEAARVFYERLGFDELGVVPGYYQGREAALRMSRRLALG"
     gene            3432..4292
                     /locus_tag="VGE92_00035"
     CDS             3432..4292
                     /locus_tag="VGE92_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY1312229.1"
                     /translation="MPGLARSAVFGSTGREVAAIGQGTWKIEQTAADAAIAALRRGLD
                     LGLTHIDTAEMYGSGAAESVIAQAIDGRRDEVFLVSKVLPSNASKRGTVNACEQSLSR
                     LKTDRLDCYLLHWRGSYPLQETIAAFDALLRDGKILSWGVSNFDVADLEEVAAIAGRG
                     HPSCNQVLYHLQERAIEHAVLPWCRQHGTALVAYTPFGQSTQAYDPKSAPGGVLAQIA
                     AAHAATARQVALAFLLRHREVFVIPKASSISHVTENAGAAALKLSAAEIGKIEGAFAL
                     GRPPRGLPMI"
     gene            complement(4289..5431)
                     /locus_tag="VGE92_00040"
     CDS             complement(4289..5431)
                     /locus_tag="VGE92_00040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEY1312230.1"
                     /translation="MAPILSIDDLRQLARRRIPRAIFDYAAGGSYEERTMMRNSADLD
                     AMTFRQRVMVDVSNVSVASTMLGIPVSMPLAIGPTGLAGLFHADGEILAARAAAACGI
                     PYCMSTMSICSIEDVRAATRQPFWFQQYLMKDRGFNQELIDRAAAAQCSALMLTLDLQ
                     VLGERRRDPRNGLTIPPRLSLRNAIDVASKPTWALKVLFGKRRTFGNLAGRIGGSSGV
                     KTLAEWTATQFDASANWRDVEWVRSRWPGKLILKGVLEAEDAKLALAAGADAIVVSNH
                     GGRQLDGAPSSISVLPEIVAAIDGRCEVMFDGGIRSGQDIAKALALGARGTLIGRSFL
                     YALAAAGEAGVRKAIEIMRNELRVTLALTGTSSAGAAGPHILRMPA"
     gene            5488..>6306
                     /locus_tag="VGE92_00045"
     CDS             5488..>6306
                     /locus_tag="VGE92_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312231.1"
                     /translation="MRKDYSNPPPPPWLQTLPRLATLYREWTGHNRKRRLFEISSLLL
                     VIAAVGATWPVWMHRVSVASAFERLKDHTLAIAALAAMVAAVAVSRQRSIERARAARS
                     WVAALPVRPRARLIEGLSLVLAPLSVGLALVLGFALLADALLAIAGLPVQGCLIAIGW
                     LLLGALLGATIGFAVRLPKPVTPYPGSRYVPHRATPGRRPVPSVGSLGIWPIRRMFAM
                     LQPKTVSRTLLPVLLMTPLGTSAAAAMLMIGLVGTALAALFLAHSVVAVTQLARR"
BASE COUNT         1121 a         1997 c         2053 g         1135 t
ORIGIN      
        1 gtcacgcgcc gatcgatggg gtttcgacaa tcaagtgtac ggctggacgc cggacggcac
       61 cgcgatcctg ttcagatcgg cgcgtgacgg cttcacgctg accgactcga agctctacac
      121 cgtgccgatg aacggcggcg ccgccagcgc gctgcccatg cccgtgtcgg gcgccggata
      181 cttctcgccg gatggcaaga aggtcgtgta ctcgccgcta tggcgcgatt ttcgcagcga
      241 gaaacgctat caaggcggtt gggccaatga cttgttcatc ttcgacctgg cgcatccgtc
      301 attgctgcaa gtcaccaacg acccgcgcac cgatcgcgat cccatgtgga tcggcgatgc
      361 gatctacttc aattcggacc gcaccggcgt cttcaatctg tatcgctacg acatcgcctc
      421 gcggcagacg cgccaactga ctcactccac cgattgggac gtgcgctggc cgagcgccga
      481 tgccgagggg cagatcgtgt acgagtccga tggcgagctg cacctctacg atacgcgcac
      541 cgatcaagac cgtgcgctgt cgatcaccgt tccggccgat gccaccacga cccggccgca
      601 aaccgtcaat gcggccgaca atatcgagag catgagcgta agtccgggcg gcgagcgact
      661 ggccatggtg gcgcgcggcg acgtgttctc cgtgccggtg gaacacggga ttacccgcaa
      721 cctgacccaa tcctcgtccg cgcacgatcg cgaggccgcc tggtcgagcg acggcaagcg
      781 cctggcgtat gtgtcggacc gcagcggcga ggaggaaatc tacgttcagg accaggacgg
      841 cagcacgccc gcggttcagg tcacgtcggg atcgagcgcg cgctacttcg cgccgcgctg
      901 gtcgggcgac aaccggcgca tcgcgttttg cgatcacacc ggcagcttgt tcgtggtcga
      961 ggtggccacg cagcggcgca acctcgtcgc gcatgacccc gcggacttat cgcaggacta
     1021 tgcctggtcg ccggacggac aatttctcgg cttttcgttg aacgacgcca acggcttttc
     1081 gagcgtcgag atttggtcgg cggcggacgg caagatgcgg cgggtcacgc cgcagtcgtt
     1141 caatgcgcac tcgccggcat gggctccgca cggcgagctt ctgtacttct tgagcgaacg
     1201 cgagtatcag ccgctgctca gcaccattga attcgatttt gccaccgatc ggcaaaccgg
     1261 gattttcgcg ctgaccctgc gcaaggacgg caagaacccg tatggcatac gcgacgatga
     1321 acccggcgat gaaaagaagg acgccgacaa ggaagagccc ggcaaagaca agaagaaacc
     1381 accggcgccg gtgcgcatcg atttcgctgg catcgagcag cgcgcgattc gcgtgccctt
     1441 ggagccggac aatctcagca acctgcaagt gggcgaagac agcctgctct accagcgcag
     1501 cgggccgatc tactacgggc gcgattcgag cgtcaaagcg agcgtcatgg cctattcgat
     1561 caaggatcgc gaggccaagc cggtggtgga agatgtgacc gaatggtcgg cgacggccga
     1621 cggcaagcac gtgctcgcgc aactctcgtc caaggagatc aagtatttcg aggtcggcaa
     1681 gagcgacgat gccaagccgg tttccttggc cggcttgatc actacgcgtg tcccggccga
     1741 ggagtggggc gagatttttg ccgatgtatg gcgccgctat cgcgattact tttacgtcgc
     1801 gaacatgcat ggctacgact ggcagaagct cagggaaaaa taccagccgc tggtgggcat
     1861 cgtgggttcg agagccgacc tcaactacgt cattgccgag atgatctcgg aattgacggt
     1921 gcagcacgcc tacatcgagg gcggcgatct gggcttgccg aagcggcctt tcgtcgcgct
     1981 gcccggggcg cgcttcgagc tcgacgctcg cgccggacgc tatcgaatcg cgaagatatt
     2041 cgccggcgag aacgaggaag agcattaccg ctcaccgttg accgaagtgg gcgtggacgt
     2101 gcacgtcggc gactacctgc tcgcgatcaa cggccgcgag ctgaaggccg gcaccgatcc
     2161 ttatgagttg ctgcgggcgg cgcccaatca gccggtggag tggcgcgtct cggcgagcgc
     2221 cgacggcagg gcggcgcgca gcatccgcta tcaaccgctc gcgagcgaga cgaacctctt
     2281 gtacctcgcc tgggtcagcg agaatcgggc gcgcgtcgat cgcttgagcc agggccgcct
     2341 gggctacatt cacataccgg acatgggcga ggccggcatc cgtgaattca tcaaatggtg
     2401 gtatccgcaa gtgcgcaaag agggcttggt ggtcgacgtg cgcgacaacg gcggcggcaa
     2461 tatctccgaa ttgttgatcg aaaggctggc gcgcactctg cacggcacga gctttgcacg
     2521 caatcaccag acgacggata cgtatccgcg cgtggtgcag ccgggaccga aggtggccct
     2581 gatcaatgag ggtaccgcct ccgacggcga tattttctca cacgaatttc gccaatggaa
     2641 aatcggcccg ctgatcggca aacgcacctg gggcggggtg gttggtatca ccgatcacgg
     2701 tccgctgctc gacggtggca tggtgtttgt gccagagttc ggtacggcgg atgagaatgg
     2761 tcattggatc atcgagggac acggtgtgga tccggatatc gtcatcgagc aggacccggt
     2821 cgaagtcttg aaagggcgcg accctcaatt ggagcgcggc gtcgccgaac ttctgcagcg
     2881 tttgccgcgc accccgcgcg gcttgccgca acgcgacgcg ccgccgatca agaccggcgc
     2941 tccctagacg tgtcggaact ctccacattg ggactcgcgt gcgcactcga tgcgcgcgaa
     3001 attgccgaaa tgtcgcgcga cctgatcgag cacggactga cctggtcctg gacgccggcc
     3061 cgggtgcaac atttcatatg cggaccggaa tcctcggtgg tggttgcgcg ccgcgagcgg
     3121 cacattgcgg cattcgccat catgcatttc ggtgatgagg tggctcattt gaacctgctg
     3181 gccgtggcgc cggagcatcg ccgccagggt ctcgggcggc aactcatgag ctggctcacc
     3241 gagaccgcga tcatcgccgg ggtgtttcgc atcaacctcg aactgcgcac tcataacgaa
     3301 gcggcgcgcg ttttctacga gcgtctgggc ttcgatgagc tcggcgtggt gccgggttat
     3361 tatcaaggcc gggaagcggc attgcgcatg tcgcgccgcc tcgccttggg ttagagagac
     3421 ccgccgcaag tatgcccggc ctggcgcgca gcgcggtatt cggcagcacc ggacgcgagg
     3481 tcgctgcgat cggtcaaggc acctggaaaa ttgagcaaac cgcggcggat gctgcgatcg
     3541 cggcgctgcg ccgcggtctt gatctcgggc tgacccacat cgataccgcc gagatgtacg
     3601 gctcgggggc ggccgaatcc gtgatcgccc aggccatcga cgggcggcgc gacgaggttt
     3661 tcttggtctc caaagtgctg cccagcaatg cctccaagcg cggcacggtg aacgcctgcg
     3721 agcaatccct gtcgcggctc aagaccgatc ggctcgactg ctacctgctg cactggcgcg
     3781 gttcatatcc gctgcaggaa acgatcgccg ctttcgacgc attgctgcgc gacggcaaga
     3841 tcctgtcgtg gggagtcagt aatttcgacg tcgccgacct ggaagaagtc gcggcgatcg
     3901 cgggtagagg acatccgtcg tgcaaccaag tgttgtacca cctgcaggag cgcgccatcg
     3961 agcacgcggt gttgccgtgg tgccgccagc acggcacggc attggtggcc tacacgccgt
     4021 tcggccaatc gacgcaggcc tacgatccga agtccgcgcc gggcggcgtg ctggcgcaga
     4081 tcgcggcggc ccacgctgcc actgcgcgcc aggttgcttt ggcgtttctc ttgcgccatc
     4141 gggaggtgtt cgtgattccg aaggcatcga gcatctcgca tgtcaccgag aatgccggcg
     4201 cggctgcgct caagttgagt gccgccgaga tcgggaaaat cgagggggca ttcgcgctcg
     4261 gccggccgcc ccgtggatta ccgatgatct aggccggcat ccgcaagatg tgcggacccg
     4321 ccgcgcccgc gctcgacgtc ccggtcagcg ccaacgtcac ccgcaactcg ttgcgcatga
     4381 tctcgatggc cttcctgacc cccgcctcgc cggcggcggc gagtgcgtac aggaacgacc
     4441 ggccgatcaa ggtgccgcgc gcgccgagcg ccagcgcctt cgcgatgtcc tggcccgagc
     4501 gaatgccgcc gtcgaacatg acttcgcagc ggccgtcgat cgccgcgacg atttccggca
     4561 gcaccgaaat cgaggatggc gcgccgtcga gctgccgccc gccgtgattc gagacgacga
     4621 ttgcgtctgc gccggcggcc agtgcgagct tcgcgtcttc ggcctcgagc actcccttca
     4681 ggatcaattt tccgggccag cggcttcgca cccactccac gtcgcgccaa ttcgcggagg
     4741 catcgaactg cgtcgcggtc cactccgcga gggtcttgac gccgctcgaa ccgccgattc
     4801 gccccgccaa attcccgaag gtgcggcgtt taccgaataa gaccttgagt gcccacgtcg
     4861 gtttgctggc cacatcgatg gcattgcgca gcgacagacg cggcgggatg gtgagcccat
     4921 tgcgcggatc gcggcggcgc tcgccgagca cctgcaggtc gagggtgagc atcagcgccg
     4981 aacactgtgc ggcggccgcg cggtcgatga gttcctgatt gaagccgcga tccttcatca
     5041 agtattgctg gaaccagaac ggctgccggg tcgcggcgcg cacatcttcg atcgaacaga
     5101 tcgacatggt gctcatgcag tagggtatgc cgcaggcggc cgcggcccgc gccgcgagaa
     5161 tctcgccgtc cgcgtggaac aatccggcaa gtccggtcgg accgatcgcc aacggcatgg
     5221 acaccggaat gccgagcatg gtgctcgcca ccgacacatt ggatacatcg accatcacgc
     5281 gttgacgaaa cgtcatggca tccaaatcgg cgctgttgcg catcatggtt cgttcctcgt
     5341 atgacccgcc ggcggcatag tcgaagatgg cgcgcggaat gcgccgccgc gccaactgcc
     5401 gtaaatcgtc gatactcagg atgggtgcca taatcgcgcc tcactatagc aaggcgagtg
     5461 ttcgccgggc ggttgcagtc gattcgcatg cgcaaagatt attcaaaccc gcctccgccg
     5521 ccatggctgc aaacgctgcc gcgtctggcc acgctgtatc gggaatggac tgggcacaat
     5581 cgcaagcggc ggctgttcga aatctcgagt ctgctgctgg tcatcgccgc agtcggcgcg
     5641 acgtggccgg tgtggatgca ccgagtaagc gtcgcatcgg cattcgaacg cttgaaggat
     5701 cacaccctgg cgatcgcagc gctcgccgcg atggtggcgg ccgtcgccgt gtcgcgccaa
     5761 aggagcattg agcgcgcgcg tgccgcgcgc tcgtgggtgg ctgcattgcc ggtgaggcct
     5821 cgcgctcgcc tgatcgaagg actctcgctt gtattggctc cgctcagcgt cggccttgca
     5881 ttggtcttgg gctttgcgct ccttgccgat gcgctgctcg cgatcgcggg attgcccgtg
     5941 cagggctgcc tcatcgcgat tggttggctc ctcttgggtg ccttgcttgg cgccaccata
     6001 ggctttgcgg tgcgccttcc gaagccggta actccctatc caggatcgcg ctatgtcccg
     6061 catcgcgcga cacccggccg gcgcccggtg ccgtcggtgg gctcacttgg aatctggccg
     6121 attcgccgga tgtttgccat gctgcagccg aaaaccgtct cgcggaccct gctgcccgtg
     6181 ctgttgatga cacccctggg aacctctgcc gcggcggcaa tgctcatgat tggcctggtt
     6241 ggcacggcgc tggctgccct gttcctggcg cactcggtgg tggcggtcac acagctcgcg
     6301 cgccgg
//
LOCUS       DASWZT010000003         2700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_82344, whole genome shotgun sequence.
ACCESSION   DASWZT010000003 DASWZT010000000
VERSION     DASWZT010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82344"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VGE92_00050"
     CDS             complement(<1..492)
                     /locus_tag="VGE92_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312232.1"
                     /translation="MVGAALLGALSSAASAYTATLSAASPQTVYLQVGVGTFTNPPYI
                     NGGQPANNTTINTVSATVAASAVGTGAAQAMTTNSTQTQSFWDNYIYCSVPGQVYIGG
                     FYRTTGGTTTAALVTATVPAALTDTTGDTLPFSKIQWTSSGNGDSGAEPFPAGTFVNG
                     GVQN"
     gene            complement(580..1218)
                     /locus_tag="VGE92_00055"
     CDS             complement(580..1218)
                     /locus_tag="VGE92_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312233.1"
                     /translation="MKVSIALKSLLGAGALTLPLLAFSESNVQTGAATASPGATAHLD
                     FSIVIPKVLYLRVGTGSAYTTGALTSVNTIDLITFTPAAGTVGNGTAVAGTGGDLTGG
                     VETAAIVSNSGNVTLNATASGALTDTTTDTIPFTQITTAATTLTSTTALPAPTLSNGT
                     SSNVVLTAPATKLIVQDAKWTYSYANAANVPAGTYGGVNTGNSRVVYTATMP"
     gene            complement(1459..1836)
                     /locus_tag="VGE92_00060"
     CDS             complement(1459..1836)
                     /locus_tag="VGE92_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312234.1"
                     /translation="MTPRNPLLKCTAFNGLLSVAALLPLMAAADSHLQSTAPGAALSA
                     QAHVDFKIVIPEVLYLRSGAADKVSIMSNSRNVTLSATAPESAAHLGSHVILRASAGK
                     VIAQDADCTLPKSRPVICTASMP"
     gene            1942..>2700
                     /locus_tag="VGE92_00065"
     CDS             1942..>2700
                     /locus_tag="VGE92_00065"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY1312235.1"
                     /translation="MSARLTLPLCAMLSALAAANPSASADSATELSAIVVTATRVPES
                     SFDLPVSVDRIERSAIAVGQLEVNLSESLLAVPGVSAQSRQNYAQDLQLSVRGFGARS
                     SFGVRGIRLYSDGIPGTMPDGQGQFSQFDLGSADHIEVLRGPFSALYGNSSGGVIAVF
                     TEDAKPGHLVEGSADYGTFNTQRYLLKTTGDDGVVNYVIDAAHFQTDGYREHSNAERD
                     NFNAKMHFATSETAHLSVIANVVETPSVQDPLGLT"
BASE COUNT          475 a          800 c          897 g          528 t
ORIGIN      
        1 gttctgcacg ccgccattga cgaacgtgcc cgcgggaaat ggttcggcgc cgctgtcgcc
       61 gttgccgctc gaggtccatt gaatcttgga gaacggcagc gtatcgccgg tcgtgtcggt
      121 caatgcggca ggcacggtgg cggtgaccag cgccgcggtc gtggtgccgc ccgtggtgcg
      181 atagaagccg ccgatgtaca cctggcccgg aacgctgcag tagatatagt tatcccaaaa
      241 actctgcgtc tgcgtgctgt tggtcgtcat cgcctgtgcg gcgccggtcc ctaccgcgct
      301 cgcggccacc gtggcggaca ccgtgttgat ggtggtgttg ttggccggct gaccgccgtt
      361 gatgtacggc ggattggtga acgtgccgac gccgacttgc agatacacgg tctgaggcga
      421 agccgcgctc agcgtcgccg tgtaggcaga ggcggcgctc gacagcgcgc ccaagagagc
      481 tgcgccgacc attcggcgca gcttcggtct ctcgaatcgc acatggcgtg ccggcgatgc
      541 agggttgccg cgcatggaaa tgccgtgtgc ttgcgggaat tacggcatcg ttgccgtgta
      601 aaccacgcgg ctgttgccgg tgttgacacc gccgtacgta ccggccggca cgttggcagc
      661 gttggcgtag ctgtaggtcc acttcgcgtc ttgcacgatc agtttggtgg ccggcgcggt
      721 gagaaccacg ttcgagctcg tgccgttcga caacgtcggc gccggcagcg cagtggtcga
      781 tgtcagggtg gttgccgccg tcgtgatctg cgtgaacgga atggtgtcgg tcgtggtatc
      841 ggtgagcgcg cccgacgccg tggcgttcaa cgtgacgttg ccgctgttgc tgacgatggc
      901 cgcggtttcg acaccgccgg tgagatcgcc gcccgtaccg gcgacggcgg tgccgttgcc
      961 gacggtgccg gcggccggag tgaaggtgat cagatcaatg gtgttgacgg acgtcagcgc
     1021 cccggtcgta taggcagagc cggtgccgac gcgcaagtac agaactttcg gaatcacaat
     1081 ggaaaaatcc aggtgggcag tcgcgccggg tgaagcggtc gcggctcccg tctgaacatt
     1141 agattcagag aacgccagga gcggaagcgt cagtgctcct gcgcccaaca aactcttcaa
     1201 ggcgatcgat actttcatgt ctgcttcctg tgctgtgaga aattcatgcg cctgtatgcg
     1261 cggggccaat actagggatt taccttaatg gcacgttgaa ccagttcgca gttcgcttcg
     1321 cgcgccttca agatccacgc gcggcggccg ataacccctg gtttttgtgc gccagttcgc
     1381 acaaaagcgc gtgcgcgagc gcgtgtgacg cgcggcacgg cgcactcgac acgcggcatg
     1441 aggaggcgcg tcggcgcctt agggcatcga ggcggtacag atgacggggc ggctcttcgg
     1501 cagcgtgcaa tccgcatctt gcgcgatcac cttgcccgcc gaggctcgca ggatgacgtg
     1561 gctgcctaag tgcgccgcgc tctcgggcgc ggtcgcgctc aaggtcacgt tgcggctatt
     1621 gctcatgatc gacaccttgt cggcggcgcc cgaacgcagg taaagcactt cgggaatcac
     1681 gatcttgaag tccacgtgag cttgagcgct cagtgcggcg ccgggcgctg tgctctgcag
     1741 gtgcgaatcc gccgccgcca tcaacggcag cagagcggcg acgctcaaca ggccgttgaa
     1801 cgcagtgcat ttcaagagcg ggttgcgtgg cgtcacctgg accgtatcga ccttgaacgc
     1861 cggtacttta atgattatct ctagcgacgc gcatgagcgg gcagcgttac gcttctgaca
     1921 tattccaaaa gcgaggggat catgagtgca cgtcttacgt taccgctgtg cgcgatgttg
     1981 tcggcgttgg ccgcggcgaa tccgagcgcg tcggccgaca gcgccacaga attatcggcc
     2041 atcgtggtca cggcgacgcg cgttccggaa tcgagcttcg atctgccggt gtccgtcgat
     2101 cgtatcgaac gctccgcgat tgccgtaggc cagcttgaag tcaatctatc ggagtcgctg
     2161 ctcgcggtgc ccggcgtatc ggcacagagc cggcagaatt acgcgcaaga cctgcagctc
     2221 tcggtgcgcg gattcggcgc gcgctcgagt ttcggcgtac gcggaatccg gctttactcc
     2281 gacggcattc cgggcacgat gccggacggc caaggccaat tctcgcaatt cgatttgggc
     2341 tcggcggatc atatcgaagt cttgcgtggc ccattctcgg cgttgtacgg caattcatcg
     2401 ggaggtgtga tcgcggtgtt caccgaagat gcgaagccgg ggcatctcgt cgagggcagc
     2461 gccgactacg gcaccttcaa cacgcagcgc tacttgctga aaaccacggg cgacgatggc
     2521 gtagtcaact atgtgatcga cgcggcgcat tttcaaaccg acgggtatcg cgagcacagc
     2581 aatgcggagc gcgacaactt caatgccaag atgcacttcg ccaccagcga aacggcgcac
     2641 ttgtcggtca tcgccaatgt ggtcgagacg ccgagcgttc aggatccgct cggcctgacc
//
LOCUS       DASWZT010000004         6541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_19409, whole genome shotgun sequence.
ACCESSION   DASWZT010000004 DASWZT010000000
VERSION     DASWZT010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6541
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19409"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VGE92_00070"
     CDS             complement(<1..834)
                     /locus_tag="VGE92_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY1312236.1"
                     /translation="MRQSNNLIAALAVALVWGPAQAEVKDPLPDSLSLGGVTLYATID
                     VGYAYQTNGVPLSSQYLGGLEYQAFTTTRNFSGSQSTIAESGLEQSKIGIRVSEPIVD
                     DFTLVARTDTGFNPLSGQLVDACGALYTNSTKPQGQQTANADSSRCGQAFNGVIWGGV
                     SSKTYGTLSIGRQQSLQLDALAVYDPQTLSYAFSFLGYSGFNAGSGSTEAARLDNSAK
                     YAYVYGPVRVSALYSNGGQDVGVLGKSYGVDVGVTFQGLSVDAVYQNEKGAVNLRSSL
                     DN"
     gene            1105..4575
                     /locus_tag="VGE92_00075"
     CDS             1105..4575
                     /locus_tag="VGE92_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HEY1312237.1"
                     /translation="MISSWLLSLLGLTYLGCLFGIAFYGERQRTYPNQRRLRPYIYSL
                     ALGVYCTSWTFFGAVGSAVRDGWGYLPIYLGPALVFLFALPFMERLARIGRAHKVSSI
                     ADFIASRFGKSRALAVLVTVISLSAAIPYIALQYKAVATSVDVLTAVTARHVPWYRDT
                     ALAVALMMALFTVLFGARRVDATRHREGLMLAIAFESVLKLLAFVAVGVFACLHLRGR
                     PWVLPVQLAQSATLLNGNTLIVTLLAAGAIFCLPRQFQVAVVECARTEDLKTARWLLP
                     AYLGIFSAVVIPVVALGTIDGLSLHTASDSLILTLPMSYGVPWLTVLVFLGGLSAATA
                     MVVVSSTALATMISNDIAAPLLWRQRLEDGASLGKRVLWVRRAVIVSLALLAFAYYRS
                     ASGSISLAAIGQLAFAAVAQFAPAMFAALYWRSASRAGVFWGMLSGFTAWALLLFLPN
                     LAGGIVAAHGFAWPDVLRLLPHSLADAAGGDAFGTRALLALSLNVLVMLIVSARRGAT
                     LQERLAARAFVAPKQPAVGLYAINCKVGDLETVASRILGGPAATQALREYVAQTRRAL
                     PKPSEPADRGLVKHVERALAASIGASSARVVLTHALHRKGLEVDEVADLLDETSQELR
                     FSRQLLQATMENVTQGISVVDAQLHVVAWNRRYLELFDYPDGLVTVGRPIVKLLRWNA
                     ARGELGPGDPEAQVEKRLAHLRAGTAYTFQRVRRNGQVYSIHGQPMAGGGFVTTYTDI
                     TDFKRHEQALLEAKQGLEERVAHRTSELSAALEAQRLAKLEAEAANKSKTRFFAAASH
                     DLLQPLNAARLFISALESQARAYPEVQELASRIDDSMRAAEELLTDLLDSARLDSGVL
                     KPDVISFPIIDLLEELRRQYAPLAQARKLELRIVGSDEVVRSDRSLLRRILQNYLSNA
                     LRYTERGGVLVGCRRRGGELEIAVYDTGPGIRMHQRQRMYAEFSRLEHDSPWGEKGLG
                     LGLSICDRLAKLMQHTLSFASNPGHGSMFGIRVTRDRNQRTRSIGHHVHAQDPSSLQG
                     LRVLCVDNDAAILDGMAALLGQWGIHVLKAGNSAEATTLCDQSEVDTVLADYHLGDGV
                     DGIELLQRLRKSGTPTLTAALISADHDAELILAARNAGFPLLHKPLRPAALRALLCAF
                     RRQARHASAA"
     gene            4595..5272
                     /locus_tag="VGE92_00080"
     CDS             4595..5272
                     /locus_tag="VGE92_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY1312238.1"
                     /translation="MNYEVLIVDDHPLYRLALKGAVAAACTNCEIFEADSVAGLFDAL
                     DRHPHADLLLLDLNLPGAYGFNALAHLRGSRPELPIIVVSASDDARTIRQALAFGAQS
                     FVSKSADAATIGENIRAVLCGEAVSHAGAILERESAVDQGALEIGHRMAQLTPQQFRV
                     LGMLCAGMLNKQIGEDLQITEATVKAHMTVILRKLGAANRTQAVLLAGRLTRESSEIK
                     PLPEDVE"
     gene            complement(5299..5808)
                     /locus_tag="VGE92_00085"
     CDS             complement(5299..5808)
                     /locus_tag="VGE92_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312239.1"
                     /translation="MTALVVGTLTYGINCNNDYAQTQVAYTAPTTSETPTPTPQVTLT
                     SNAATQVVGQTVTLSWDSKNTESCTASGGEANDGWTGSLALSGSMQITESTGGSTTYA
                     ITCAGAPPAAIAKAIVDFTEAAGSGSGSPGTSKGGGGAMSAPWLLLLSLPVAAGVRRR
                     RLSRVVEQR"
     gene            5834..6172
                     /locus_tag="VGE92_00090"
     CDS             5834..6172
                     /locus_tag="VGE92_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312240.1"
                     /translation="MPLQPSLPLPPVAVHCGPDEFHVSVTGVLIAALIALAEIDVGGG
                     ACTVTFVDACTTWLPVPQVIEYVKVPAVVSITGIDPAGEILLPPQLTPPEASTDVQGE
                     TVEFHVKAVT"
BASE COUNT         1156 a         2019 c         2015 g         1351 t
ORIGIN      
        1 gttgtcgagg gaacttctca ggttgacggc gcccttttca ttctggtaaa cggcgtcgac
       61 ggacagtccc tggaaagtca cgccgacatc cacgccatac gatttgccga gcacaccgac
      121 atcctggccg ccgttcgagt acagggccga cacacgcacg gggccgtaga catacgcata
      181 cttggccgag ttgtccaagc gcgccgcctc cgtactcccc gagcctgcgt tgaaccccga
      241 atagccgaga aacgagaacg cataggagag agtttgcgga tcgtacacgg cgagcgcgtc
      301 gagctgcagc gactgttgcc ggccgatgct gagcgtgcca taggtcttgc tgctcacacc
      361 gccccagatc acgccgttga aggcttgacc gcagcggctg ctatcggcat tcgcagtttg
      421 ctgtccttgc ggcttggtgc tgttcgtgta gagggctccg caggcgtcga ccaactggcc
      481 cgacagcgga ttgaagccgg tatcggttct cgccacgagg gtgaagtcgt cgacgatggg
      541 ttcggacacc cggatgccga tcttggattg ctcgaggccg ctttccgcga tggtcgactg
      601 cgagcccgag aaatttcgcg tcgtcgtaaa ggcctgatat tcgaggccgc ctaggtactg
      661 gctgctgagc ggaacaccgt tggtctggta ggcgtaaccc acatcaatcg tggcatatag
      721 cgtgactccg cccagggaca gactgtcagg caacgggtcc ttcacctcgg cctgtgctgg
      781 gccccacacc aacgccacgg caagtgccgc aatcaagttg ttgctctgtc tcattagttt
      841 ctccctcagc cgagttatcc ccaaagcgcg cctgtaccgg cgatcgcctc cagcccacgg
      901 agctattcgt tccggcgtcc gcttccggct gcgcatagca gttattgatg gctcaggatc
      961 gcggaacaat agtaggtcca taccttcccg caacgccatt cgactttggt cgatagccgc
     1021 ttgctttcga gggcccgcgt gccagggtca tgccatttgt gttagtccga tcgcgagctt
     1081 agagtggggc tgaggggtgt agccatgatt tcgagctggc ttctgtcatt gctcgggttg
     1141 acatatctcg gctgtctgtt cggtatcgcg ttctacggcg aacgccaacg aacctatccc
     1201 aaccaacgcc gcctgcgacc ctatatatac tcgctcgcac tcggtgtgta ctgcacgtcc
     1261 tggacattct ttggtgcggt cggctccgcc gtgcgcgacg gctggggtta tctgccgatt
     1321 tacctaggac ccgcgctggt attccttttc gcgctgcctt tcatggagcg gctggcgcgg
     1381 attggacgcg cccacaaggt cagttcgatc gcagatttca tcgcctctcg gttcggcaag
     1441 agccgcgctc tggccgtatt ggtgaccgtg atctcgctat cggccgccat cccgtacatt
     1501 gccctgcaat acaaagcagt cgccaccagt gtcgatgtgc tgaccgcggt gactgcgcgc
     1561 catgttccat ggtatcgcga tactgccctc gcggtcgcgc tgatgatggc gctgttcacg
     1621 gtgctattcg gcgcgcggcg ggtcgatgca actcgacacc gcgaaggatt gatgctggcg
     1681 attgcatttg aatcggtgct gaagctgctg gcgttcgtgg cggtcggcgt atttgcgtgc
     1741 ctgcatctgc gcggccgccc ttgggtgcta cctgtgcagc tcgcacaaag cgcaaccctg
     1801 cttaacggca ataccttgat cgttacgctg ctcgcagcag gagcgatttt ctgtctgccg
     1861 cggcagttcc aagtcgcggt ggtcgagtgc gcgcgcacgg aggacctcaa gaccgcgcgc
     1921 tggttattgc ctgcgtacct cggaatcttc tctgcagtcg tcattcctgt tgtggcactt
     1981 ggcacgatcg acgggctctc gctgcatacc gcgtcggatt ctctgattct gacgctgccg
     2041 atgagctatg gagtcccttg gcttaccgta ttggtgtttc tgggcggtct gtcggcggcc
     2101 acggccatgg tggtggtctc cagcaccgca ttggccacga tgatctcgaa tgacatagcc
     2161 gcgccactgt tgtggcggca acgactggag gatggcgcga gcctgggaaa gcgcgtgctg
     2221 tgggtcaggc gtgccgtcat cgtgtcgctc gcgctgttgg cctttgccta ctaccgctcc
     2281 gcgtcgggct cgatcagcct ggccgcgatc ggtcagctgg cctttgccgc ggtggcacaa
     2341 tttgcgccgg ccatgtttgc cgcgctctat tggagaagtg ccagccgtgc gggtgtattt
     2401 tggggaatgt tgagcggctt caccgcctgg gccctcctac tattcctgcc caatctcgcc
     2461 ggaggaatcg tggcggcaca cggtttcgca tggcccgatg tacttcgttt gctgccgcat
     2521 tcgctggccg atgccgcagg aggagatgct ttcggaactc gcgcgctctt ggcgctgtcg
     2581 ctgaatgtac tcgtgatgct catcgtttcg gcacgccgcg gcgcgactct ccaggaacgc
     2641 ttggccgcca gggccttcgt tgcccccaaa cagcccgcgg tcgggctgta cgcgatcaac
     2701 tgcaaagtgg gcgatctcga gactgtggca tcgaggatac taggcggtcc tgccgccacc
     2761 caggcgcttc gcgaatatgt cgctcaaacc cgtcgcgctc tgccgaagcc gtcggaacct
     2821 gccgatcggg gactcgtgaa acatgtcgag cgcgcattgg ctgcgtcgat cggcgcctca
     2881 tcggcgcgcg tcgtgctcac gcatgccctg caccgcaagg gcctcgaagt cgatgaagtc
     2941 gccgacctac tcgacgagac ctcgcaagag ttgcgtttca gccgacagtt gctgcaggcg
     3001 acgatggaaa acgtcacgca agggatcagc gtggtcgacg cgcaattgca tgtggtggca
     3061 tggaatcggc gctatctgga gctatttgac tatcccgacg gactggtcac ggtcgggcgc
     3121 ccgatcgtca aattgctgcg atggaatgcc gcgcgcggcg agctcggacc gggagatccc
     3181 gaggctcaag tcgaaaaacg gctggcgcac ctgcgcgccg gcactgccta tacgtttcaa
     3241 cgagtgcgtc gcaacggaca ggtgtacagc attcacggtc aaccgatggc aggcggcggt
     3301 ttcgtcacga cttataccga catcacggat ttcaagcgcc acgaacaagc tctgctcgag
     3361 gccaagcaag ggctggagga gcgcgtcgca caccgcactt cagaactgag tgcggcgctc
     3421 gaagcgcagc gccttgccaa actggaggcc gaagccgcaa ataagagtaa gacgcgtttt
     3481 ttcgccgccg ccagtcatga tctgctgcag ccgctcaacg cggcgagact cttcatatca
     3541 gccctcgagt cgcaggcgcg ggcatatcca gaagttcagg aactggccag ccgcatcgat
     3601 gactcgatgc gcgccgcgga ggagcttctc accgatttgc tcgacagcgc gcgtctggac
     3661 agcggcgtgc tcaaaccgga tgtcatcagc tttccgatca tcgatctgct cgaggaactg
     3721 cgccgccaat atgcgccgct cgcacaggct cgaaagttgg aacttcgcat cgtgggcagc
     3781 gatgaagtcg tgcgtagcga tcgctccttg ctgcggcgga tcttgcaaaa ctatctctcc
     3841 aatgcgctgc gctacacgga gcgtggcggt gtgctggtcg gatgccgcag gcgcggcggc
     3901 gaactcgaaa ttgccgtgta tgacaccggt cccggaatac gaatgcatca acgccaaagg
     3961 atgtacgctg agttcagtcg gctggaacat gactcgccat ggggcgagaa gggcctggga
     4021 cttggcttgt cgatttgcga tcggctggcc aagctcatgc agcacacact gtcattcgcc
     4081 agcaatccgg gccacggctc catgttcggt atacgagtaa cgcgtgaccg taaccaacgc
     4141 acgcgcagca tcggccatca cgtgcacgcg caggacccga gcagcctgca gggcctgcgc
     4201 gtgctgtgcg tcgacaacga cgccgcgatt ctcgatggaa tggcggcgct gcttgggcag
     4261 tggggtattc acgtgctgaa ggccggcaat agcgccgaag ccaccacgtt gtgcgaccaa
     4321 tccgaagtcg acaccgtcct tgccgactat catctgggcg acggcgtcga cggtatcgaa
     4381 ttgctgcagc ggttgcgcaa atcgggaacg ccgacactca ccgccgcact catcagtgcc
     4441 gatcacgacg ccgagctgat cctggccgcg cgcaacgcgg gatttcccct cctgcacaag
     4501 ccgctgcggc ccgcagcgct gcgcgcccta ctctgcgcgt tccgacggca ggcgcgacat
     4561 gcctcggcgg cgtgagccgc tcgcaagtgg accgatgaac tacgaagtac tcatcgtcga
     4621 tgaccatccg ctgtatcgcc tggcgctgaa gggagcggtc gccgcggcct gcacgaactg
     4681 cgagatattc gaggccgaca gtgtcgccgg ccttttcgac gcgctcgatc ggcaccctca
     4741 tgcggatttg ctgcttctgg acctcaacct tcccggcgcc tatgggttca acgcactcgc
     4801 acatctgcgc ggctcacgcc cggagcttcc catcatcgtg gtctcggcat ccgacgatgc
     4861 gcgcaccata cgccaagcat tggcttttgg cgcacagagc ttcgtgtcca aatccgccga
     4921 cgcggccacc atcggtgaaa atatccgggc ggtgctgtgt ggcgaggccg tatcgcacgc
     4981 cggcgcgata ctcgagaggg aatctgccgt cgaccagggt gcgttggaga tcggtcatcg
     5041 catggcgcaa ctgacgccgc agcagtttcg ggtgcttggc atgttgtgcg ccggaatgct
     5101 caacaagcaa atcggcgagg atcttcagat cacggaggcg accgtcaagg cgcatatgac
     5161 cgtgatcctg cgcaaactgg gcgccgcgaa tcgcacgcag gccgttctgc tcgcagggcg
     5221 ccttacgcgc gaatcgagcg aaatcaaacc attgcccgag gacgtcgagt gaacggcgcg
     5281 ctgcttgcgg atctgcaact acctctgttc tacgacacgg ctcagtctgc ggcgtcgcac
     5341 tcctgccgcg acaggcagcg acagaagcag cagccacggc gcactcatcg ctccgccgcc
     5401 gcccttgctg gtgccgggcg acccggatcc ggaaccggca gcctcggtaa aatcaacgat
     5461 cgcttttgcg atcgcggcag gaggcgcgcc cgcgcatgtg attgcgtagg tcgtcgatcc
     5521 gccagtcgac tcggttatct gcatcgaccc cgataacgcc agagatcctg tccagccgtc
     5581 gttcgcttcg ccccccgagg ctgtgcacga ttcggtattt ttggagtccc aactcagcgt
     5641 cacggtttga ccgacaactt gcgtggcagc gttgctggtg agtgtcactt ggggtgtcgg
     5701 cgtgggagtc tcgctcgtgg tcggtgcggt ataggcgacc tgcgtctgtg cgtagtcgtt
     5761 attgcagttg atgccgtacg taagggtccc gaccaccaat gcggtcaccg ttgccgtgcc
     5821 cgaggaattc ttagtgccgc tccagccatc gctgccgtta cccccggtcg ccgtacactg
     5881 cgggcccgat gaattccacg tcagcgtcac cggcgtgttg attgcggcgc tcatcgcgct
     5941 ggctgagatc gacgtcggcg gtggcgcctg cacggtcact ttcgtcgacg cgtgcaccac
     6001 ctggctcccg gtaccgcaag tgatcgaata cgtgaaggta cccgccgtgg tctcgataac
     6061 cggaattgat ccggccggcg agatattgct gccgccccaa ttgacgccgc ccgaggcatc
     6121 caccgacgtg cagggcgaga cagtggaatt ccatgtcaaa gcagttactt gacccacagg
     6181 aacactgctg gggcttgcgc taattgcgac agctgcgggc ggtacggtga cagtcagctg
     6241 ggcttgaccc acggatatgc cagcgccgca cgtgagtgta aacgtgtaat tgccgggtac
     6301 gctctctgtc accgcctccg aagcctgtcc cgcctggtag tttcctgccc aaccatcgcc
     6361 ggcgacgccg cccgtttgag tgcatggcgt ggtgttggtg gtccagaaaa gcgggtactg
     6421 ataatttgcg acccacgtcg taccgttggt cgatgtcagt gtcaccttgg gagtcgtgtt
     6481 cgtccaagta atcgttgtgc tggcttggta ggcgccacac tgaagctggt aggtgtactg
     6541 t
//
LOCUS       DASWZT010000005         1736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_92005, whole genome shotgun sequence.
ACCESSION   DASWZT010000005 DASWZT010000000
VERSION     DASWZT010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92005"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VGE92_00095"
     CDS             complement(<1..767)
                     /locus_tag="VGE92_00095"
                     /inference="COORDINATES: protein motif:HMM:NF026240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HEY1312241.1"
                     /translation="MDADTGAYVPITDMRWGKSTLALDSWRLKKAKVSAFVPQGRKPV
                     FELRTRAGLRIRATANHPFLKLKQWVPLGELRPGDRIAVARDIPVFGKTPIPDWEASL
                     LGLMISEGQCDTPGHSPTYTCADPALVQTLRACVADSGMGVVPEYNRAHLWLKSYGLN
                     VGAGEKFVPQSVFMAPENSVRLFLQSLFSGDGSVYHSKAGVFLEYYSKSRRLIEDVHH
                     LLLRFGVFSLIREKTTAIGTPACKIQITDKDQIRRFA"
     gene            complement(792..1124)
                     /gene="dcd"
                     /locus_tag="VGE92_00100"
                     /pseudo
     CDS             complement(792..1124)
                     /gene="dcd"
                     /locus_tag="VGE92_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985727.1"
                     /note="Catalyzes the formation of dUTP from dCTP in
                     thymidylate biosynthesis; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
     gene            complement(1124..>1736)
                     /locus_tag="VGE92_00105"
     CDS             complement(1124..>1736)
                     /locus_tag="VGE92_00105"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEY1312242.1"
                     /translation="LTPLRAHGVEVMSIGFMIDQEQPMAWRGPMVTQALTQLLGDTSW
                     GELDYLVVDMPPGTGDIQLTLAQRVPVSGAVIVTTPQDIALLDARKGLKMFEKVEVRV
                     LGVVENMSLHVCSQCGHVEHIFGSGGGARMAAQYGVQLLGELPLDIRIREDADGGCPT
                     VVAEPGSTRALAYLQMARRTAARLATLNKDYSRAFPKITVEAT"
BASE COUNT          346 a          539 c          512 g          339 t
ORIGIN      
        1 tcggcaaacc gccgtatctg gtccttgtcg gtgatttgaa tcttgcatgc cggcgttcca
       61 atcgcagtcg ttttttcccg aatcagcgaa aatacgccga aacgcagcag caggtgatgc
      121 acatcttcga tcagccttcg ggatttggag tagtactcga gaaacacgcc ggctttggag
      181 tgataaacac tgccgtctcc gctgaacaac gactgcaaga acaaccgcac cgagttctcg
      241 ggtgccatga acaccgactg aggaacgaac ttctcgcccg ctccaacatt cagtccatag
      301 ctcttaagcc agagatgcgc ccgattgtac tcgggcacca ctcccattcc cgagtctgcg
      361 acgcacgctc gcaaggtctg aaccaatgcc ggatcggcgc aggtgtacgt cgggctatgg
      421 ccaggagtgt cgcattgccc ctcggaaatc atgagtccga gcaggctcgc ttcccaatcg
      481 ggaatgggag tcttgccgaa gaccgggata tcgcgggcca cggcgatgcg atcgccggga
      541 cgaagctcgc cgagtggcac ccattgcttc aatttgagaa acggatgatt cgcggtcgcg
      601 cgaatgcgca atcccgctcg tgtgcgaagc tcgaagacag gctttcgccc ttgcgggaca
      661 aaggccgaaa ctttggcttt tttgagccgc caggaatcga gcgcgagcgt cgacttgccc
      721 cagcgcatgt cggtaattgg cacatacgca ccggtatccg cgtccacgac ccgagtgtcg
      781 ccggtgacgc acttgccgag acaagcgacc aaaacgttgc gcggaatccg aaaaaactcc
      841 accgtgctcg caagcgcaaa ggagttcggc gggatgatgc agaccggccc tctgaaatcc
      901 acgaacgact tctcgtcgaa attcttcgga tcgacgatgg tcgagttgat gttggtgaag
      961 atcttgaact catcggtgca gcgcacgtcg tagccgtagc tcgaggtgcc gtaggagatg
     1021 atcttatggc cgtccgcgac gcgcacctgg tcgggcgaga acggctcgat catgcggtgc
     1081 tgcgccgcca tgcgcctgat ccaacgatcg ctcttgatgc tcattacgtc gcttccaccg
     1141 taatcttggg aaatgcccgg ctgtagtcct tgttgagggt cgccaaccgc gccgccgtgc
     1201 gccgggccat ctgcaagtac gcgagcgcgc gcgtgctgcc gggctcggcc accacggtgg
     1261 ggcagccgcc gtccgcgtcc tcgcggattc gaatgtccaa gggtagctcg ccgagcaatt
     1321 gcacgccgta ctgtgccgcc atgcgcgcac cgccgccgct gccgaagatg tgctcgacat
     1381 gaccgcactg gctgcacacg tgcaggctca tgttctccac caccccgagc acgcgcactt
     1441 cgactttctc gaacatcttg aggcccttgc gtgcatcgag gagagcgatg tcctgcgggg
     1501 tggttacgat gaccgcgccg ctcacgggaa ctcgctgcgc cagggtgagc tgaatgtcgc
     1561 cggtgccggg cggcatgtcg accaccaagt aatccagttc gccccagctg gtgtcgccca
     1621 acagctgagt gagcgcctgg gtcaccatcg gtccgcgcca cgccatcggt tgttcttgat
     1681 cgatcatgaa cccgatcgac atcacttcga cgccatgtgc gcgcaatggc gtgaga
//
LOCUS       DASWZT010000006         2070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_69, whole genome shotgun sequence.
ACCESSION   DASWZT010000006 DASWZT010000000
VERSION     DASWZT010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VGE92_00110"
     CDS             <1..285
                     /locus_tag="VGE92_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312243.1"
                     /translation="PDKAGFRVDARTRDGEIQSDFPELKIDNNDHQATASGSVGNGSS
                     HIVINNEHDGIEIRKLSASAAPAPPAPPAPPGKPGKALPAPKAKVEPTEN"
     gene            complement(713..1456)
                     /gene="cmk"
                     /locus_tag="VGE92_00115"
     CDS             complement(713..1456)
                     /gene="cmk"
                     /locus_tag="VGE92_00115"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEY1312244.1"
                     /translation="MGAKSQRRVQSSREMTGPSDLLQNSSRKLIIAIDGPAGAGKSTI
                     ASRLARKLGYVNLESGAMYRALALKAIEQDLSFDDEAALLQLAQKSRIQIEPALDGNR
                     TLINGHDVTARIRERDVTEAASRVSVHPQVRAWMVARQREMGAGGGVVMEGRDIGTKV
                     FPDADLKIFLDADPVVREQRRMQQQNIKGEVAANVAADLRERDHRDRTRAVSPLKAAD
                     DAVLIDSTALSEAEVLQKVEALAGERLRE"
     gene            1579..>2070
                     /locus_tag="VGE92_00120"
     CDS             1579..>2070
                     /locus_tag="VGE92_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312245.1"
                     /translation="MAFAAGQNASQNTTPETARDPGVREGRLGAGSPSKGLTPAQQAI
                     FSAGLTSFRQLASVTGSVADTRIGLGPRFNALSCAQCHAQPAVGGSSAEINPQISAAI
                     AQGAANKIPSFINSRSPVRVARFPYAADLRQADGSVHALFTISGRSDAPGCRLAQPDF
                     KQAA"
BASE COUNT          375 a          668 c          640 g          387 t
ORIGIN      
        1 cccgataaag ctggattccg tgtggacgcg cgcacgcgag atggagagat tcagtcggac
       61 ttcccggagt tgaagatcga caacaacgat caccaggcca cggccagcgg cagcgtgggc
      121 aatggctcgt cgcacatcgt gatcaacaac gagcacgacg gcatcgagat tcgcaagctc
      181 tcggccagcg ctgcgcccgc tccgcctgcg cctccggcgc cgcccgggaa gccagggaag
      241 gctttgcccg cccccaaggc gaaagtggag ccgacggaga actaaagcgt ggcgccggca
      301 tccggctgcg cccgagggcc tcctcgccct cggcgcggcg ttatccacgc tctcgccggt
      361 gaagaccaca atcaaggaac acgatgacct ttaccccggc acatctcgag gaggggttcg
      421 cgactacctg ggcgaaacga cggtgggcga ggcggctgcg cgctggagaa tcacgcgagc
      481 ccatctttct cgaatcctca acgccacatg ccgggatcac ggcgccgatg ggattgcgcc
      541 tgtcggccgg tccgggtcca gcccccgacg tctggctgcg catgcaggtg cagccatcat
      601 tggctggcgc aacgagcgcg gcaacccaag gttcgcccgt tccctcatgg gatcgcgaaa
      661 cgggcttccg tcgcccgcgc ataaaatagt accgcttgag cggataccgg actcactcgc
      721 gcaatcgctc gccggccaac gcttccacct tctgcagaac ttccgcctcg ctgagcgcag
      781 tcgaatcgat aagaacggca tcgtcagcgg ctttgagcgg agaaaccgcg cgggtgcggt
      841 cgcggtggtc gcgctcgcgc aggtccgcgg ctacgttggc agcgacctcc cccttgatgt
      901 tttgctgctg catgcggcgc tgctcgcgga ccacgggatc ggcgtcgaga aatatcttca
      961 ggtcggcgtc gggaaaaact ttggtgccga tgtcgcggcc ttccatgacc acgccgccgc
     1021 ctgcgcccat ctcgcgctgc cgcgccacca tccaggcgcg tacctgggga tgaaccgaaa
     1081 cgcgcgaggc cgcttcggtg acatcgcgct cgcggatgcg cgcggtgacg tcgtgcccat
     1141 tgatcagggt acggttgccg tcgagagcag gctcgatctg aatacgcgat ttttgcgcca
     1201 gttgcagcag cgcggcttca tcgtcgaagg ataaatcctg ctcaatggct ttcagcgcca
     1261 gggcgcggta catggctccg ctctcgaggt tcacgtagcc gagtttgcgc gcgaggcggg
     1321 aggcgatggt gctcttgccc gcgcccgccg ggccgtcgat ggcaataata agtttgcggg
     1381 aagaattttg cagcaggtcg gatgggccgg tcatttcgcg actagattgt acacggcgct
     1441 gcgattttgc tcccaccttt cgatttaaaa tgatatcggc agtttgcccc tgaaatgcgc
     1501 acactccgtt cgacgaggat tcgatccgca ggtcgattta ggcgcgtcat cacttcgttc
     1561 ctcagggcgc ttttcttcgt ggctttcgcc gctgggcaaa atgcatcgca gaacacaacg
     1621 ccagagaccg ctcgtgaccc tggggtgcgc gagggacgcc tgggtgcggg atcacccagc
     1681 aaaggcttaa ccccggcgca gcaagctatt ttttctgccg gcttgaccag cttccggcaa
     1741 cttgcttcgg tgaccggctc ggtcgccgat acccgaatag ggctcggccc taggttcaat
     1801 gcactctctt gcgcgcagtg ccatgcccag cctgcggtcg gcgggagcag cgccgaaatc
     1861 aatcctcaaa tcagcgccgc catcgcgcag ggcgcggcga ataaaatacc ttcttttatt
     1921 aattcgcgca gcccagtgcg tgtggcccgc tttccttacg ccgccgacct gcgccaggct
     1981 gatggcagcg ttcatgcttt gttcacgatc agcggccgca gcgacgctcc cggctgcagg
     2041 ctcgcgcagc ctgatttcaa acaggctgca
//
LOCUS       DASWZT010000007         2330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_48464, whole genome shotgun sequence.
ACCESSION   DASWZT010000007 DASWZT010000000
VERSION     DASWZT010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48464"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VGE92_00125"
     CDS             complement(<1..599)
                     /locus_tag="VGE92_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312246.1"
                     /translation="MSPDEERLMQLFQNRAGLKKAYADLKDEFHLLSDRLKQQEGATI
                     RVQEQLDALGDLLGDPKTGFSALVFFQLRALWKTCHQQLAAFATELTRQQEARETAKH
                     NADTEAAKNARLADVAQRVQAAQAAADVQRKALGAAQSELARLTAFWHYFKRRRGQKT
                     LEVLRSQVAIAEAVIGDLHTERSAIEHAASVEFPGISLAA"
     gene            complement(713..1072)
                     /locus_tag="VGE92_00130"
     CDS             complement(713..1072)
                     /locus_tag="VGE92_00130"
                     /inference="COORDINATES: protein motif:HMM:NF018552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HEY1312247.1"
                     /translation="MRLGWLVKDLRGVTARRRSRVPKDCDLYLTPLELSRYTSVFRLT
                     YEFEFDAGPINDPDLEVCVYHDARLAEVRSFRGFQRHPQLSKLHSGLKRELDQRWTRN
                     MMLNKWLEYCSERGHHF"
     gene            complement(1191..2012)
                     /locus_tag="VGE92_00135"
     CDS             complement(1191..2012)
                     /locus_tag="VGE92_00135"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015269492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEY1312248.1"
                     /translation="MKRTVFFVSDQTGITAETMGHSLMTQFDGIEFRQVTVPFIATVD
                     KAIEAVRKIDLVGMQEGSRPIIFSTLVQEDVRDVVRGSSGFFLDFFDPFLAPLENELK
                     TQSTHIASRTHRTADTHAYATRIDATNFALANDDGSVVRDYERADVILVGVSRSGKTP
                     TCLYLAMQYGIFAANYPLTEDDLEARCLPPALDPYARKLFGLTIKPDRLQQIRNERRP
                     DSRYSSAPQVAFEVRAAESLFERHGIPMIDATDVSIEEISSRILDRTGIERRLRP"
     gene            2090..>2330
                     /locus_tag="VGE92_00140"
     CDS             2090..>2330
                     /locus_tag="VGE92_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEY1312249.1"
                     /translation="MKPYIIPFEQLKNKDVELVGGKNASIGEMISGLATLGVSVPGGF
                     ATTSHAYRDFLAQAGLDERIREVLAALDVDDVERLA"
BASE COUNT          456 a          707 c          685 g          482 t
ORIGIN      
        1 gcggccagtg atattcccgg aaattcgacg ctcgcggcat gttcgatggc actgcgctcg
       61 gtgtgcagat cgccgatgac cgcctcggcg atcgcaactt ggctgcgcag cacctccaga
      121 gtcttttggc cgcggcggcg cttgaagtaa tgccagaagg cggtcaagcg cgcgagttcg
      181 ctctgtgcgg cgcccaaggc cttgcgctgc acgtcggcgg cggcttgcgc tgcctgcacg
      241 cgttgcgcga cgtccgcgag ccgcgcgttc ttcgccgcct cggtgtctgc attgtgtttt
      301 gcggtctcgc gcgcctcttg ctggcgcgtg agttcggtgg cgaacgccgc caattgctgg
      361 tgacaggtct tccacaatgc gcgcagctga aaaaatacca gggcgctgaa gcccgtcttc
      421 gggtcgccga gcaagtcgcc gagggcgtcg agttgctcct gcacgcgaat ggtcgcgcct
      481 tcctgctgct tgagacgatc gctcagcagg tgaaattcgt ccttgaggtc ggcgtaggct
      541 ttcttcaagc ccgcgcgatt ttggaagagc tgcatgagcc gctcttcgtc aggactcacg
      601 gcacctccgt ccaaccctcc tttgagaaaa ctcagcttat cgatcaaagt cccgccttga
      661 gccacagggt tatcgagcgg gaaatcgcca ccaacctagg gcgctcgagc cgctagaagt
      721 gatgcccccg ctccgagcaa tattccagcc atttatttaa catcatgttc cgcgtccagc
      781 gctgatccag ttcacgcttg agtccagagt gcagcttcga gagctgcgga tggcgctgga
      841 agccgcgaaa actgcgcact tcggccagcc gcgcgtcgtg atacacgcag acttccaaat
      901 ctgggtcgtt gatcggaccg gcgtcgaatt cgaactcata tgtgagacga aacacactcg
      961 tatagcggct caattcgagc ggggtgagat agagatcaca gtctttgggc acgcgcgacc
     1021 tgcggcgcgc cgtcaccccg cgcagatcct tcaccagcca gccgagacgc acgaaattgc
     1081 tctcatacag cgtcatcaag ctgacgaagc tgccgggctt ggctctccag gttgtcgcac
     1141 accattgatc cgctaacatg tctcgaacta taccacgcac atccagaatg tcagggccgc
     1201 aagcgccgtt cgatgccggt gcggtcgaga attctgctgg agatttcttc gatcgagacg
     1261 tccgtcgcat cgatcattgg aataccgtgc cgttcgaaca acgactcggc cgctctgacc
     1321 tcgaaggcga cttgcggcgc cgacgaatag cggctatcgg ggcggcgctc attgcgaatt
     1381 tgttgcagcc gatcgggctt gatcgtcagg ccgaataatt tgcgcgcgta cgggtcgagc
     1441 gccggcggca agcagcgggc ttccagatcg tcctcggtca acggataatt cgccgcaaat
     1501 attccgtact gcatcgccag atacaggcag gtcggggtct tacccgagcg cgacacgccg
     1561 accagaatca cgtcggcgcg ctcgtagtcg cgcaccaccg aaccgtcatc gttcgccagc
     1621 gcgaaattcg tggcatcgat gcgcgtcgca taggcgtgcg tatcggcggt gcggtgcgta
     1681 cggctggcga tgtgcgtcga ctgcgtcttc agttcgtttt ccagcggcgc gaggaacgga
     1741 tcgaagaaat ccagaaaaaa tccgctgctg ccgcgaacca cgtcgcgaac atcctcttgc
     1801 accagggtac tgaaaatgat tggccgtgaa ccctcttgca ttccgaccag atcgatcttg
     1861 cgaacggcct caatcgcctt gtcaaccgtg gcaataaatg gcacagtcac ctggcgaaat
     1921 tcgatgccgt cgaattgtgt cataaggctg tggcccattg tttctgcggt aatgccggtt
     1981 tgatcggaaa caaaaaacac agttcgttta atcacggtag cctcaggtgt tggcgcagcg
     2041 tcgccggcaa agtgtttcac ttaacccccc gccccacaag cagcgcacta tgaagcccta
     2101 tatcatcccg ttcgagcagt tgaagaataa ggacgttgag ctggtcgggg gcaagaacgc
     2161 ttccatcggc gagatgattt ccggcctcgc gactcttggc gtgtcggtgc ccggcggctt
     2221 tgcgaccaca tcacatgcgt accgcgactt tctggcgcaa gccggcctcg acgaacgcat
     2281 ccgcgaggtg ctcgctgcat tggacgtcga cgatgtcgag cggctcgcgg
//
LOCUS       DASWZT010000008         2404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_87168, whole genome shotgun sequence.
ACCESSION   DASWZT010000008 DASWZT010000000
VERSION     DASWZT010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87168"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="VGE92_00145"
     CDS             complement(<1..981)
                     /locus_tag="VGE92_00145"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEY1312250.1"
                     /translation="MSSDIGIAQQATLKPIGEIAARLGIPAASIENYGHYKAKIALDY
                     IETLKGKPDGKLILVTALSPTPAGEGKTTTTVGLGDALNKIGKKAVICLREPSLGPVF
                     GMKGGAAGGGYAQVVPMEDINLHFTGDFSAIGLAHNLLSALIDNHIHHGNELGFDVRR
                     IAWKRVMDMNDRALRQITVGLGGPGNGFPREDGFDIVVASEVMAIFCLAKSLADLKQR
                     LGNIVVGYRRDLTPIHARDLKAQGAMTVLLKEALKPNLVQTLENNPAFIHGGPFANIA
                     HGCNSVLATTTALKLADYVVTEAGFGADLGAEKFIDIKCRKSGLRPAAVVL"
     gene            complement(1110..2105)
                     /locus_tag="VGE92_00150"
     CDS             complement(1110..2105)
                     /locus_tag="VGE92_00150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEY1312251.1"
                     /translation="MSIPREMTVVEIAAPGGPEQLRPAQRPVPSPKDGEVLVRVAAAG
                     VNRPDVMQRQGRYPPPAGASDLPGLEIAGEVVALGPHVSTLSIGDAVTALLPGGGYAA
                     YAIAAAPLCLPVPAGISMVEAAAIPETFFTVWTNLFDRGRCKAGDTVLIHGGTSGIGT
                     TAIQLASAWGARVFATAGSDDKARACERLGAVRGINYRTEDFVAVMREATQDKGVNVT
                     LDMVAGSYVARNLEIAALEGRVVTISLLGGSRAEINMGLILTKRLTLTGSTLRSRTVA
                     QKAEVADAVRKNVWPLLTAGRVRPVIHATFPLAEASEAHRLMETSNHIGKIVLTV"
     gene            2221..>2404
                     /locus_tag="VGE92_00155"
     CDS             2221..>2404
                     /locus_tag="VGE92_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312252.1"
                     /translation="MAGDLLWNCWQTGQTLANGLPEQLRPVTREDGYAIQALLEQRSA
                     APLYGWKIAATSQAGQA"
BASE COUNT          427 a          787 c          750 g          440 t
ORIGIN      
        1 gagcaccacc gccgcgggcc gcaggcccga cttgcggcac ttgatgtcga tgaatttctc
       61 cgcacccagg tcggcgccga atcccgcttc ggtcaccacg taatcggcca atttcaatgc
      121 cgtggtcgtc gccagcaccg aattacagcc atgggcgatg ttggcaaaag gtccgccatg
      181 gatgaaggcc ggattattct cgagggtttg taccaaattc ggtttgagcg cctccttgag
      241 cagtaccgtc atcgcgccct gggccttcag gtcgcgggcg tggatgggcg tgaggtcgcg
      301 ccggtagccg accacgatgt tgcccaagcg ctgcttcaaa tccgccaggc tcttcgccag
      361 gcagaagatg gccatcacct cggatgcaac cacgatgtcg aaaccatcct cgcgaggaaa
      421 gccattgccg ggaccgccca gccccacggt gatctggcgc aaggcgcggt cattcatatc
      481 catgacgcgc ttccacgcga tgcgccgaac gtcgaaaccc agctcattgc catgatggat
      541 gtgattgtcg atgagcgcgg agagcagatt gtgcgcgagc ccgatcgcac taaagtcacc
      601 ggtaaagtgc aggttgatgt cttccatcgg tacgacttgc gcatagccgc cgccggcggc
      661 tccgcccttc atgccgaaca cggggcctaa gctcggctcg cgcaggcaaa tcaccgcttt
      721 cttgccgatc ttgttcaggg catcgccgag accgaccgtg gtcgtcgtct tgccttcgcc
      781 cgcgggtgtc ggactcaacg cggtgaccag gatgagcttg ccatccggtt tgcctttcag
      841 cgtctcgatg taatcgagcg cgatttttgc tttgtagtgg ccgtagtttt cgatgctggc
      901 agccggaatc cctaagcgcg cggcgatttc gccgatgggt ttaagggttg cttgttgagc
      961 gatgccgata tcggaactca cgatttctcc agtgttgtcg cggcccggtg ccgcgttaag
     1021 ggacgccggg ccgtgtcatc ggccgacgca tgcgtagcaa acaagtatag caaacgcggt
     1081 gctccgcggc gcgcgcagcg ccgatgtttt tagaccgtca gaacgatttt cccgatgtga
     1141 ttcgaggtct ccatcagccg atgcgcctcg ctcgcctcgg ccagcggaaa cgtcgcatga
     1201 atcacgggcc gaacccgacc ggccgtgagc aacggccaga cgttcttgcg gaccgcgtcg
     1261 gcaacctcgg ccttctgggc gaccgtcctc gagcgcaagg tcgagccggt cagcgtcaag
     1321 cgtttggtga ggatcaatcc catgttgatt tccgcccgcg atccgcccag caacgaaatc
     1381 gtcacgactc ggccctcgag ggcggcgatc tccaaattgc gcgcaacgta actgccggcc
     1441 accatgtcca aggtgacatt gacgccctta tcctgggtgg cctcgcgcat gaccgcgacg
     1501 aagtcctcgg tgcggtaatt gattccgcgc accgcgccca agcgttcgca ggcgcgcgcc
     1561 ttgtcgtccg atcccgcggt cgcgaacacg cgtgcacccc acgcagaggc gagctggatt
     1621 gccgtggtgc caataccgct ggtgccgcca tggatcaaca cggtgtcgcc ggccttgcag
     1681 cggccgcgat cgaagaggtt ggtccaaacc gtgaagaagg tctccggaat ggctgccgct
     1741 tcgaccatgc tgatccccgc gggcacgggc aggcagagcg gcgccgcggc aatggcatac
     1801 gctgcgtagc cgccgcccgg caacagcgca gtcaccgcat cgccgatcga cagcgtcgac
     1861 acgtgcggtc cgagtgcaac cacttcgccg gcaatttcca gccccggcaa atcggaggca
     1921 cccgcgggcg gcggatagcg gccctggcgc tgcatcacgt cgggccggtt caccccggcc
     1981 gcggcaacgc gcaccagcac ttcgccgtcc ttgggactcg gcaccggccg ctgcgccggc
     2041 cgcaactgct ccgggccgcc gggggcggcg atctcgacca ccgtcatttc gcgaggtatc
     2101 gacatgatga ctccagctga ggcgacttca cggcgccggc cgcgaataat acaggctcgc
     2161 accgcacccg cgcgcgcctt tgtctgttac gtttgcggga tgcggaccca tgcggcgcaa
     2221 ttggccggcg atctcctgtg gaattgttgg cagacgggac agactctggc aaatggcttg
     2281 cccgagcagc ttcggcccgt gacccgggag gacggctatg cgattcaagc gctgctcgag
     2341 cagcgcagtg ccgcgccgct gtacggctgg aaaattgcgg ccaccagcca ggccggccag
     2401 gcac
//
LOCUS       DASWZT010000009         3978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_67821, whole genome shotgun sequence.
ACCESSION   DASWZT010000009 DASWZT010000000
VERSION     DASWZT010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67821"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VGE92_00160"
     CDS             <1..530
                     /locus_tag="VGE92_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312253.1"
                     /translation="TCAARIARRFIETLQPGDTTCAGKVPPLRLVPRFAVHAAELEPA
                     VAAPGNRATAAQLHWLSAAVQTVGDVLTRVDGNGAGPGVGLRGGSFRAGDGGAIVHVS
                     LNGVRWTEDLSVSGTIEKPAARIGTVRASVHVTSAGGLTGDLEIDWPAGAAVGTAEVH
                     GTLGGVAVNATMPAP"
     gene            558..2558
                     /locus_tag="VGE92_00165"
     CDS             558..2558
                     /locus_tag="VGE92_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY1312254.1"
                     /translation="MPQEHAQAVPQGPYDAAVAALREGRAATAERMLRALEARFPGEA
                     NCRWLIGAALLDQNKIAESIATLERLLAVAPNFSAARVDLARAYRRAGRAAQARQEVR
                     RVLQHTPLHHRAWLAYGDALVDLGQHADAAVAFERARSTDPEAARVEEATAALVADDR
                     KRAELIFREILKRDASHAAALCGLAALSLIADRPRDAERLLHHALKQSAHLPLAYRGL
                     GPALLALGRAEEAQRAARHLMLIEPHSPQTWILLAGAAIRLMRQEEALDAYERAVSLQ
                     PDEVGLRMSIGHVQKTLGRRRDSEASYKRALQMDPGRAEAYSSLADLKNYHFSDAEVS
                     DIQRLLALDKRDRSNEAQLNFALGKAFEQRELYSQAFAHYSRGNALRRLDAPFDIEGF
                     ERRSARIRGLFDAAFFAAHAGGGDPSPAPIFIVGLPRSGSTLVEQILASHSRVDGTME
                     LPNILNLVSQFDDMAPSRDAYPESVATAPAAQLAALGSRYLAETAPLRRGRERFTDKL
                     PNNFSHVGLIHAILPHATIIDARRHPLDACFSTFKQYFAEGQTFSRHLDDLGRYYRCY
                     LSLMDHWDAILPGKVLHVQYEELVGDPEANIRRLLEHCGLDFEPACLNFHATQRSVRT
                     ASAEQVRQPLYTSAVGYWKHFAKELEPLRASLGDCLGRFADH"
     gene            2626..>3978
                     /locus_tag="VGE92_00170"
     CDS             2626..>3978
                     /locus_tag="VGE92_00170"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1312255.1"
                     /translation="MSTTMTHRAPAAPGRRRDTRGLYTRSKLSLAVAAAIYGAGVHGL
                     AALAAGASDTALEEITVTARKRAENLQDVPLSIDVYTAKDLKNLAISSFDDYAEKVPS
                     MSFISTGPGTQVFVMRGVSDGSNPNYGNTSATGFFVDDMSMSFNSAQPDLHLYDIERI
                     EVLNGPQGTTFGAGAMSGAVRYITNKPDVKSFSGGLDFDGGKIQSAQNNWTYEGFFNA
                     PLIEGVLGLRLSAFSDSHGGFINNQLTTRTWVNGAVSTNAAWAGKDYNREHIEGGRVA
                     LKWVINEGWSGLLTYSYQRQHTRGAWDEDPTLPPRTVERFGPEANDFEAKTLDFHVDG
                     DVGIGDLVFASTYWSQPRHQWDEYSQYEQNFNAGAKNTPPTGYPGTQEGFTCLNDPYY
                     GGAPFSGCNPATQYYTYFIHPQRWSEELRLVSKPGGRFHWLAGLYWERTQDKLYGEDY
                     Y"
BASE COUNT          726 a         1310 c         1268 g          674 t
ORIGIN      
        1 gcacctgcgc cgcgcgcatc gcacgccgct tcatcgaaac actgcaaccc ggcgacacga
       61 cttgcgccgg caaggtcccg ccgttgcgct tggtgccgcg gtttgctgtg catgccgccg
      121 agctcgagcc cgccgtggcc gcgccgggaa accgcgccac cgcggcgcag ttgcactggc
      181 tgagcgcggc ggtgcagacc gtcggcgatg tgctgacgcg tgtcgacggc aatggcgccg
      241 gcccaggggt gggtctgcgc ggcgggagtt ttcgcgcggg cgatggcggc gccatcgtgc
      301 atgtcagctt gaacggggtg cgttggacag aggatctgtc ggtgagcggc accatcgaga
      361 agccggcagc gcggattgga accgtgcgcg catcggtcca cgtcacctcg gcgggcggcc
      421 ttaccggcga tcttgagatc gactggcccg caggcgccgc ggtcgggaca gccgaggttc
      481 acggaacact gggcggcgtc gccgtgaacg caacgatgcc tgcgccttaa ggccgccagt
      541 tgccgtaaag tagccggatg ccgcaagagc atgcacaagc tgttccgcag ggcccctatg
      601 acgccgctgt ggcggcgctg cgcgagggac gggcggcaac cgccgaacgc atgttgcgcg
      661 ctctcgaggc gcgctttccc ggcgaggcga actgccggtg gctgatcggc gcggcgctcc
      721 tcgatcagaa caagatcgcc gagagcatcg ccacactcga acggttgctg gctgtcgcgc
      781 cgaatttttc cgctgcacgc gtggatctcg cccgcgccta tcgccgcgcc ggccgcgccg
      841 cgcaggcacg tcaggaagtg cggcgcgttc tgcagcacac gcccctgcat caccgcgcat
      901 ggctcgctta cggcgatgcc ttggtcgacc tcgggcagca tgcggacgcg gccgtcgcct
      961 tcgagcgcgc acgctctacc gatccggagg cggcgcgtgt cgaggaggcg acggcggcgc
     1021 tcgtcgccga cgaccgcaaa cgcgcggagc tcattttccg ggaaatcctc aagcgcgatg
     1081 cgagtcacgc cgcggcgctg tgcggcctcg cggcgctgtc actcatagcc gacagacccc
     1141 gcgatgccga gcgcctgctg catcacgcgt tgaagcaatc cgcgcatctt ccgcttgcct
     1201 atcgtggact cggccctgcg ctgctggcct tgggccgcgc cgaggaagcg caacgcgccg
     1261 cgcgccacct catgttgatc gagccccaca gtccgcaaac ctggatcctg ctggccggcg
     1321 ccgcaatacg gctcatgcgt caggaggagg cgctcgacgc ctacgaacga gcggtgagtc
     1381 tgcagccgga tgaagtgggg ctgcgcatgt ccattggcca cgtgcaaaag actctcggcc
     1441 ggcgccgcga cagcgaagcc tcctataaac gcgccctgca gatggatccg ggacgcgccg
     1501 aggcctactc gagtcttgca gacctcaaga actatcactt cagcgacgcg gaggtctcgg
     1561 acattcagcg gctgctcgcg ctggataagc gcgaccggtc gaacgaggct cagctgaatt
     1621 tcgctctcgg gaaggccttc gagcaacgcg agctctactc gcaggctttt gcgcactatt
     1681 cccggggtaa cgctctgcgg cgcctcgatg ctcccttcga catcgaaggc ttcgagcgcc
     1741 gcagcgccag gatccgcggc ttgtttgatg cggcgttctt tgccgcgcat gcgggcggcg
     1801 gggatccctc gcccgcaccc atcttcattg tcggactgcc gcgttccgga tctacgctgg
     1861 tggaacagat cctcgccagc cactcgcgcg tcgatggcac catggagttg cccaatatcc
     1921 tcaacctggt gagccagttc gatgatatgg cgcctagccg cgacgcttac ccggaaagcg
     1981 tggcgaccgc gcccgcagcc caactggccg cgctcggcag ccgttacctc gcggaaacgg
     2041 cgccgctgcg ccgtggacgc gagcgcttca ccgacaagct gcccaataac ttcagtcacg
     2101 tcggactcat ccatgcgatt ctgccgcacg caacaatcat agacgcgcga cgtcacccgc
     2161 tggatgcgtg cttcagcacg ttcaaacagt attttgccga gggtcagacc ttcagccgac
     2221 atctcgacga cctcggccgc tactaccgct gctatctatc gctgatggac cactgggacg
     2281 cgatcctgcc gggcaaggtg ctgcacgtcc agtacgaaga gctggtgggc gatccggagg
     2341 cgaatatccg tcgcctgctc gagcactgcg ggctcgactt cgagcccgcc tgcctcaatt
     2401 tccacgcgac gcagcgctcg gtgcgcacgg cgagcgctga acaggtgcgt caacccttgt
     2461 acacctcggc ggtcggctac tggaagcatt tcgccaagga actggaaccg ttgcgcgcaa
     2521 gcctgggcga ttgtctcggg cgcttcgccg atcattgacg cggcctgccc gcgcaagaga
     2581 tgatcggcgc atacgctcgc gaagggcgac gcacatgggg attcgatgag caccaccatg
     2641 acgcatcggg cacctgcagc ccctggccga cgccgagata cccgcggcct ttatactcgc
     2701 tcgaaactca gtctggcggt cgccgcggca atatacgggg caggcgtgca tggccttgcg
     2761 gcgctggccg ccggcgcctc ggataccgct ctcgaagaga ttacggtgac cgcgcgcaag
     2821 cgagccgaga atctgcagga tgtaccgttg agcatcgacg tatacacggc gaaggatctg
     2881 aagaacctcg ccatcagcag cttcgatgac tatgcggaga aggtgccctc catgtccttc
     2941 atcagcaccg ggcccggtac gcaagtgttc gtcatgcgcg gagtctccga cggcagcaac
     3001 ccaaactacg ggaacacctc cgcgaccggc ttcttcgtcg acgacatgtc catgagtttc
     3061 aacagcgcgc agccggacct gcatctttac gacatcgaac gcatcgaagt cctgaatggc
     3121 ccgcagggta cgacgttcgg ggccggcgcg atgtcgggag cggtccgcta cataaccaat
     3181 aagccggacg tgaagagctt cagcggcggc ctcgactttg acggcggaaa gattcaaagc
     3241 gctcagaata actggaccta tgaggggttt ttcaacgcgc ccctgatcga gggagttctc
     3301 ggactgcggc tctcggcttt cagcgacagt cacggcggat tcatcaacaa ccagctcacg
     3361 acgcgtacct gggtcaacgg cgccgtgtcg accaacgcgg catgggcggg caaagattac
     3421 aaccgcgagc acatcgaggg cggccgcgtc gccttgaaat gggtcatcaa tgagggctgg
     3481 agcggcttgc tcacctatag ttatcagcgc cagcatacgc gcggcgcgtg ggacgaggat
     3541 ccgactctcc caccgcggac cgtcgaacgt ttcggtccag aggccaatga cttcgaggcc
     3601 aagacgctcg actttcacgt cgatggcgat gtcgggatcg gcgatctggt gtttgcgagc
     3661 acctactggt cgcagcccag gcatcagtgg gacgagtact cgcagtacga gcaaaacttc
     3721 aatgccggcg ccaagaacac accccccaca ggctatccgg gcacgcagga agggttcacc
     3781 tgtctgaacg acccttatta cgggggcgcc cccttcagtg gatgcaaccc ggcgacacag
     3841 tactacacgt acttcatcca cccgcagcgc tggtcggaag agctgcggct ggtatccaag
     3901 ccgggcggcc gatttcactg gctcgcgggt ctgtattggg agaggaccca ggacaagctc
     3961 tatggtgagg actactac
//
LOCUS       DASWZT010000010         3955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_82381, whole genome shotgun sequence.
ACCESSION   DASWZT010000010 DASWZT010000000
VERSION     DASWZT010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82381"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VGE92_00175"
     CDS             complement(<1..384)
                     /locus_tag="VGE92_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEY1312256.1"
                     /translation="MTARRFKGGETVDFIVVGSGAAGGVIARELSAAGSSVLVFEQGP
                     RFGPADFAHDELKYFFLNGITLDPALSPQSFRKTTDAVAKVPLGPSPAQYARLVGGSS
                     NHYTANFWRFHEIDFHERSVLGSISG"
     gene            complement(381..1001)
                     /locus_tag="VGE92_00180"
     CDS             complement(381..1001)
                     /locus_tag="VGE92_00180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEY1312257.1"
                     /translation="MNSSGGDQDNSRRSFLLSGGGFLTSVWLAAHWPAIDAAAHHAAA
                     VSEVPAAAGFEFLSAADAADIEAIAAQIVPSGATPGAREAHAVYFIDRALGTFFAAWA
                     ADFRPGLGDFQAKFRAANPTLAAFAKSDPGQQIAFLKTVERTPFFDSVRTLTLLGMFS
                     SPKYGGNYQGSGWRLMGFVDQHAFAPPFGYYDREYSGFVPYTDKPV"
     gene            complement(1031..2683)
                     /locus_tag="VGE92_00185"
     CDS             complement(1031..2683)
                     /locus_tag="VGE92_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY1312258.1"
                     /translation="MTGRKFKPTETVDFVVVGSGAAGAVIARELAQTGFSVVVLEQGP
                     RLTSADFEHDEFKYFYLSGITNDPARSPQTFRDDPGQVAQRPRWGNSLIYARMVGGSS
                     NHFTANYWRFHESDFKERSLLGPIAGTGFADWPISYQELEPYYTKVEWDIGVSGLAGS
                     SPFDPPRSKPYPMPPLPVKSSGALLERGARKLGLHPFPAPMAIVSQAYRGRPGCAHCG
                     FCIGFGCEVMAKSSTLYTMIPEAEATGRCEIRAASYAFQVAMNSSGRTTGVLYLDEHK
                     QQHLQKAKAVVLSANGAETARLLLNSADSRFPQGLANSSGLVGKYLMFNQGAGVHAQF
                     AHELNEYKSVQVTRILHDFYESDPRRGFYGGGGIDARMGPQPAIWALAAGGELPVWGA
                     DLKARLEAFPRAMMSTAHCTSLPVATNSVSIDPALKDAWGIPAIRVTYKDHPDDLASA
                     HFLQERAFEIMQAAGAEKVWKAPPRLARGGVHLLGTCRMGNDPAGSVIDKYHRTHDVP
                     NLFLCDGSSFVTSGRGQPTMTIQALSFRAADHIGRFAKRGEI"
     gene            2876..>3955
                     /locus_tag="VGE92_00190"
     CDS             2876..>3955
                     /locus_tag="VGE92_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312259.1"
                     /translation="MAASFLIIAWLCLGRLAFPLYVLLMPAAAFVALLVYHQRIAQKR
                     SAARRGADFYRAGLARMQDEWRGRGASGMQFDVAQHIYAADLDVFGQGGLFELLCAAR
                     TPLGESTLARWLSAPADLEAIRLRQLCIDDLRGRLDLREALATEGESSIALTAETLIA
                     WSEAPNRLNRLWIRWVARLLAGLAVATLVVWLAWGIRYPLLAVLLAEVALGYLLKQSI
                     QRALAAVERAYEDLKGLSQLLNRIEAEPFGAAPLRAMVGTLSSHSRSAAKTFAKLATI
                     VNLVESRRNPMLTPLLLLVMYPLQAALAAEGWRSLHGHVIRNWLQVLGEIEALISLAQ
                     FADEHPEYPFPEFLDGSAAFKATALG"
BASE COUNT          702 a         1259 c         1242 g          752 t
ORIGIN      
        1 cccggagatg gatccgagca cgctgcgctc gtgaaagtcg atttcgtgaa agcgccagaa
       61 attcgccgtg tagtggttgc tgctgccgcc gacgagtcgc gcgtattgcg cgggcgatgg
      121 ccccaagggg actttcgcaa cggcgtcggt ggtcttgcgg aagctctgcg gactcagcgc
      181 tggatcgagg gtgatcccat tcaaaaagaa atacttcagc tcgtcgtgcg caaaatcggc
      241 cggcccgaac cgcggcccct gctcgaatac cagcacgctc gagcccgcgg cggacaactc
      301 gcgcgcgatc acgccgcccg cggcgcccga gcccaccacg atgaaatcca ccgtctcgcc
      361 gcccttgaac cggcgcgccg tcaaacgggc ttgtccgtgt acggcacgaa accgctgtat
      421 tcgcgatcgt agtagccgaa cggcggcgcg aacgcgtgct ggtcgacgaa ccccatgaga
      481 cgccagcccg acccctggta attgccgccg tatttcggcg aggagaacat gccgagcagc
      541 gtcaatgtgc gcacgctgtc gaagaacggg gtgcgctcca cggtcttgag gaaggcgatt
      601 tgctgacccg gatccgactt cgcgaacgct gccaaggtcg gattcgcggc gcgaaatttc
      661 gcctgaaaat cgccaagacc cgggcgaaaa tcagcggccc acgccgcgaa aaacgtcccg
      721 agcgcgcgat cgatgaaata cacggcgtgc gcctcgcgcg ctcccggcgt ggcgccgctc
      781 ggcacgattt gcgcggcaat cgcctcgatg tcggccgcat cggcggcgct taagaactcg
      841 aaccctgccg ctgcggggac ctcggacacg gcagcggcat ggtgggctgc cgcatcgatt
      901 gccggccaat gcgccgcgag ccaaacgctg gtcaaaaaac cgccgccgga caagaggaac
      961 gagcgccgcg aattgtcttg gtcgccgccg ctgctattca tatgctgtct cgattgttga
     1021 tagcgtgggt tcagatctcg ccccgcttcg cgaaccggcc gatatggtcg gccgccctga
     1081 agctcagcgc ctgaatcgtc atcgtcggct gaccccgtcc ggaggtgacg aaactcgagc
     1141 cgtcgcagag aaaaagattc ggcacatcgt gcgtccgatg gtatttgtcg atgaccgatc
     1201 cggcagggtc gttgcccatg cggcaagttc cgagcaagtg cacaccgccg cgcgccaggc
     1261 gcggtggcgc cttccaaacc ttctcggcgc ccgcggcctg catgatctcg aatgcgcgct
     1321 cctgcagaaa gtgcgcgctc gccaaatcgt ccggatggtc cttgtaggtg acgcgaatgg
     1381 ccggtatgcc ccacgcgtcc ttgagcgccg gatcgatgct gacgctgttg gtcgcaacgg
     1441 gcaacgacgt gcaatgcgcg gtcgacatca tggcgcgggg aaaggcttcg agccgggcct
     1501 tcagatcggc tccccagacg ggcaattcac cccccgccgc caatgcccag atggcgggtt
     1561 gcggccccat gcgcgcgtcg attccgccac cgccgtaaaa tccgcgccgc ggatccgact
     1621 cgtagaaatc gtgcaatatg cgcgtcacct gcacgctctt gtactcgttg agctcatgcg
     1681 cgaattgtgc gtgcacgccg gcgccttggt tgaacatgag atacttgccg accaggccgc
     1741 tcgagtttgc aaggccctga ggaaagcgcg agtccgccga attgagcagc aagcgcgccg
     1801 tttctgcgcc attcgccgac agcaccaccg cctttgcttt ttgtaaatgc tgttgcttgt
     1861 gttcatcgag atacagcacg ccggttgtgc gtccgctcga attcatcgcc acctggaacg
     1921 cataactggc cgctcgaatt tcgcaacgac cggtggcttc ggcttcggga atcatcgtat
     1981 acagggtcga cgacttcgcc atgacctcgc agccaaaccc gatgcaaaat ccgcaatgcg
     2041 cgcaacccgg ccggccgcga tatgcttgcg agacaattgc catgggcgcc ggaaacggat
     2101 gcaaccccaa cttacgcgcg cctcgctcca gcagcgcgcc ggaggatttg accggcaacg
     2161 gcggcatggg atagggcttg ctgcgcggcg gatcgaaggg actcgagccg gcaagtccgg
     2221 aaacgccgat gtcccattct actttggtgt aatagggttc gagttcctgg tacgagatcg
     2281 gccagtccgc aaagccggtc ccggcaatcg gcccgagcaa gctgcgttcc ttgaaatcgc
     2341 tctcgtggaa gcgccagtag ttggccgtga agtgattgct gctgccgccc accatgcgcg
     2401 cgtagatcag cgaattgccc caacgcggcc gctgcgcgac ctggccggga tcatcgcgga
     2461 aggtctgcgg actgcgcgcc ggatcgttgg tgatgccgct caaatagaag tatttgaatt
     2521 catcgtgctc gaaatcagcc gacgtcaggc gcggcccttg ctcgagcacc acgacgctga
     2581 atcccgtctg cgccaattct cgcgctatga ccgcgcccgc cgctcccgag ccgaccacga
     2641 cgaaatcgac ggtctcggtg ggcttgaact tgcggcccgt catacatacc ctcttggcgt
     2701 cttgcccgat cctgcgctcg gcgcaaatgg cgagtcaagg gccatcggcg tttgaaatat
     2761 gccacactga ttcgtggcgt cgagcggcga atacaatcag cgtctacgcg agcgcgcagc
     2821 ggatctcgcg cgcctcgatc gactgcatgc acgcattggg agcgcgcggc ttgccgtggc
     2881 tgcctcattt ttgatcatcg cgtggctttg ccttgggcga ctggcatttc cgctttatgt
     2941 tctgttgatg ccggcggcag cgttcgttgc cttgttggta tatcaccagc gcatcgctca
     3001 gaagcgatcg gcagcgcggc gtggcgcgga cttttatcgt gcgggtctcg cgcgaatgca
     3061 ggacgaatgg cgcggccgtg gggcatccgg catgcagttc gatgttgcgc agcatattta
     3121 tgcggccgat ctggatgtgt tcgggcaagg tggcctgttc gaattgctgt gcgccgcgcg
     3181 cacgccgctc ggcgagagca cgctggcgcg ctggctctcg gcgcccgccg acctcgaggc
     3241 gattcgctta cggcagctgt gtatcgatga tctgcgcggt cggctcgact tgcgcgaggc
     3301 actggcaacc gagggcgagt cgagcatcgc gctgactgcg gaaacgctca tcgcatggtc
     3361 cgaggcgccg aaccggctga accggctgtg gatccgctgg gttgcgcggc tgcttgcagg
     3421 tctcgcggtg gcaactctgg tcgtttggct ggcctggggt attcgctacc cgttgctcgc
     3481 ggttcttctc gccgaggtcg cgctcggcta tttgctcaag caatcgattc aacgggcgct
     3541 cgcggcggtg gaacgcgctt acgaggacct caagggcctg tcgcagctgt tgaatcgcat
     3601 cgaggccgag ccattcggcg cggcgccgtt gcgcgccatg gtgggcacgc tgtcgtcgca
     3661 ttcgcgcagc gccgcgaaaa cgtttgcgaa gctcgcgacc atcgtcaatc tggtcgagtc
     3721 gcgccgcaac cccatgctca cccctctgct gttattggtg atgtatccgc tgcaagcggc
     3781 gcttgccgcc gaaggttggc gcagcctgca cggccacgtc atccgtaact ggctgcaggt
     3841 actcggcgag atcgaagcat tgatttcgct cgcccagttt gccgacgaac accccgagta
     3901 tccgtttcca gaattcttgg acggcagcgc cgccttcaag gcaaccgctt tgggc
//
LOCUS       DASWZT010000011         4677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_38821, whole genome shotgun sequence.
ACCESSION   DASWZT010000011 DASWZT010000000
VERSION     DASWZT010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38821"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="VGE92_00195"
     CDS             complement(<1..859)
                     /locus_tag="VGE92_00195"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1312260.1"
                     /translation="MTDQTHNSWVRSAGFGLGVLSFINLFSYLDRYIVSALTESLKHS
                     NMGLSDANLGSLMSGFLIVYTLTAPIFGALGDRRSRPRLIAVGVACWSFATALSGFAG
                     SYLALFLARASVGVGEAAYVTIAPSLLSDYFPARDRGRVMAIFFCAIPVGSALGYVVG
                     GLIDKHYGWRSAFFVAGAPGLLLAALCLLLRDPPRGASDSGAAPAAATPEKASNLGKD
                     TWATFGRLLHNKPYVLTVLAYAAYTFALGGLAFWMPAFLERSRGIPRSEATVSFGTIV
                     VITGFIGTFV"
     gene            922..2553
                     /locus_tag="VGE92_00200"
     CDS             922..2553
                     /locus_tag="VGE92_00200"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY1312261.1"
                     /translation="MLRKSWMFGFAAFACTVLWFTLLAGRPLFDPDEGRYAEIPREML
                     SGGDWVIPHLNALVYLEKPPLQYWLTAGALRAFGESEFAARLATGIAGYVALAVVFLL
                     GRELWGAEAGARALLYTLASSLFVLLGHQLTLDMLLCCCLTVALGCFLMAQARGDAVR
                     WRLWMLGCWAGMALGVLAKGLVGALIPAATLCIYSLWQRDWRVLGRLNLRWGLPLFAV
                     LAAPWFIAAAVRNAQFLRFFFIREHLQRFLTPIEHRTEPWWFFMPVLIVAIMPWLPQA
                     MRALAAALGKGAPRADFDAPRLLGIWCVFVLVFFSLSDSKLIPYILPAVPALALLCAS
                     QPRADSRACLLAGALLTLGCCAGILAYASGRWSTAEARALVLLIRPMLFWTSACLALG
                     ALAAALCVLSGRRLAAVTGLCGFWFLASGTILLAAIPAAALFSAKDVAMILQSRAGPA
                     DPIFAVQSYQQSLAFYLRRPVILVGYRDEFDLGLTQNPERGIATLPQFAATWLGLDGG
                     GFAVMPPRTLERLSALGVPLHEIARFPDEVIISRR"
     gene            2673..>4677
                     /locus_tag="VGE92_00205"
     CDS             2673..>4677
                     /locus_tag="VGE92_00205"
                     /inference="COORDINATES: protein motif:HMM:NF021424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY1312262.1"
                     /translation="MSIHVALNHVTHYRYDRAVGLSPQIVRLRPAPHCRTRILSYSLK
                     VQPQDHFINWQQDPQANYLARLVFPDETRELKIEVDLVAEMSVLNPFDFFLAPYAEQF
                     PFKYDSTERRELVPYLVKSPLSQPLESYLGGISRERCRTIDFVVGVNQRVANDIRYLI
                     RLEPGVQSPEETLTRSAGSCRDSAALLVQLMRHVGLAARFVSGYLVQLVADVKALDGP
                     VGATQDFTDLHAWCEVYLPGAGWIGLDPTSGLLAGEGHIPLACTPEPSAAAPVSGAVD
                     ECETTFEHRMSVRRIWEAPRVTKPYSDAQWSSIEALGRAVDADLESQDVRLTMGGEPT
                     FVSSDDPDGEEWNSAALGKNKRRLAADIYRRLKNKYAPAGLAHFGQGKWYPGEQLPRW
                     SLNCYWRKDGEPLWTKPQLLADEQAPQALTAESARRFLTRVAETLALDPKFVFPAYED
                     AFYYLWRERRLPANVDPFESRLQDPMERERLAKIFEQGMGQTIGYVLPIARTAEDERW
                     RSGAWFLRSERCYLIPGDSPIGYRLPLESQPWVAAGDFPFIHPPDPSRSFPALAAHAE
                     IRRQFRESAAVPLNETAPAAQESAAAIARTSMCAEPRNGVLYIFMPPVAELEHYLELV
                     AAVEATAEALAQPVILEGYEPPKDPRLGSFRVTPDPGVIEVNVH"
BASE COUNT          790 a         1465 c         1518 g          904 t
ORIGIN      
        1 cgacgaaagt gccgatgaaa ccggtgatga cgacgatggt gccgaagctg acggtcgcct
       61 cgctgcgcgg gattccgcgc gaacgctcga gaaaggcggg catccaaaac gccaagccgc
      121 cgagcgcgaa ggtataggcg gcataggcca aaaccgtgag cacgtacggc ttgttatgca
      181 gcagccgtcc aaaggtcgcc caggtatctt tcccgaggtt cgacgccttc tcaggtgtag
      241 ccgcggcggg cgcggcgccg gagtccgatg ctccgcgcgg cgggtcgcgc agcagcaggc
      301 acagcgcggc cagcagcaat ccgggtgctc cggcgacgaa gaacgccgag cgccagccgt
      361 agtgcttgtc gatgagtccg cccaccacat agccgagcgc cgagcccacc ggaatcgcgc
      421 aaaagaagat ggccatgacg cggccgcggt cgcgcgccgg aaaataatcc gagagcagac
      481 ttggggcgat agtcacgtag gcggcttcgc ccacgccgac cgaagcgcgc gccaagaaca
      541 gagcgaggta acttcccgca aacccggaca gggccgtggc gaagctccag cacgccacgc
      601 cgaccgcaat caggcgcggc cgggaacgcc gatctcccaa cgcgccgaaa atcggcgccg
      661 tcagggtgta gacgatcagg aacccggaca tgagcgatcc caaatttgcg tccgaaagtc
      721 ccatgttcga atgcttgagg ctctcggtga gcgccgagac gatgtagcga tccagatagc
      781 tgaacagatt gatgaacgag agcacgccca agccgaaacc cgcggagcgt acccaggagt
      841 tatgtgtttg atcggtcacg ccccggtata gtagcaatgc gagtttgcaa ttaggcttat
      901 ttgttcggcg gttctatgct catgctccgc aagtcatgga tgttcggctt tgcggcgttt
      961 gcttgcacgg tcctgtggtt cacgctgctc gcgggccggc cgctgttcga tccggacgaa
     1021 gggcgctatg ccgagattcc gcgcgagatg ttgagcggcg gcgattgggt gattccgcat
     1081 cttaacgctc tcgtgtacct ggaaaagccg ccgctgcagt attggctcac ggccggagcc
     1141 ttgcgcgcat tcggcgagag cgaattcgcg gcgcgtctcg cgacgggcat tgccggctat
     1201 gttgcacttg cggtcgtttt tctcctgggc cgtgaactgt ggggagccga agcgggagcg
     1261 cgcgcgctgc tctacacgtt ggcctcgagc ctgttcgtgc tgctcgggca tcagttgacg
     1321 ctcgacatgt tgctgtgttg ctgcctcacg gtggcattgg gttgttttct gatggcgcag
     1381 gcgcgcggcg atgccgtgcg atggcgcctg tggatgttgg gctgttgggc cggcatggcg
     1441 ctcggcgttc ttgccaaagg gctggtcggt gcgctgatcc cggccgcaac gttgtgcatc
     1501 tacagtctgt ggcaacgcga ttggcgcgtc ctcggccgct tgaatctgcg ctgggggctg
     1561 ccgttgttcg cggtgctcgc cgcgccgtgg ttcatcgcgg cggcagtccg caatgcgcaa
     1621 ttcttgaggt tcttcttcat tcgcgaacac ttgcagcggt ttctcacgcc gatcgagcat
     1681 cgcaccgagc cgtggtggtt cttcatgccg gtgctgattg tcgccatcat gccgtggctg
     1741 ccgcaggcga tgcgggcgct cgccgccgcg ctcggcaaag gtgcgccgcg cgctgatttc
     1801 gatgcgcctc ggctgctcgg catctggtgc gttttcgtcc tggtattctt ctcgctgtcc
     1861 gactcaaaat tgattccgta cattttgccc gcggtcccgg cgttggcgct gctgtgcgcc
     1921 agccaaccgc gcgccgacag ccgcgcgtgc ctgctcgcgg gtgcgctgct caccctcggt
     1981 tgctgtgccg gaatcctcgc ctacgcgagc ggccgctgga gtacggccga agcccgggcg
     2041 ctcgtgctcc tgatacgtcc catgttgttc tggaccagcg cctgcctcgc gctcggcgcg
     2101 ctggcggcgg cactgtgcgt gctgtcgggc cgacgcctcg ccgcggtgac cggcttgtgc
     2161 ggcttttggt tcctggcgtc gggcacgatt ctgctcgcag caattcccgc ggcggcattg
     2221 ttcagcgcca aggatgttgc gatgatatta cagagccggg cagggccggc ggaccccata
     2281 ttcgcggtgc agtcctatca gcagtcgctg gccttttatt tgcgccggcc ggtcatattg
     2341 gtgggctatc gcgacgaatt cgacttggga ctgactcaaa atcccgagcg aggcatcgcg
     2401 accctgccgc aattcgccgc cacctggctc ggcctcgacg gcggcggctt cgcggtcatg
     2461 ccgccgcgaa ctctggagcg cttatccgcc ctcggcgtgc cgctgcacga gatcgcgcgc
     2521 tttcccgacg aagtgataat cagtcgacgt taatggcgcg ccgcgccgga tcatggccct
     2581 acgcgcgcgg cgcacgttat acaattcccg cggagcggtc cgaactgcgt cgtgaatgcc
     2641 atgcaccgtg atggtgcgtg acggagaaat ccttgtcgat ccacgttgcc ctaaatcacg
     2701 tcacccacta tcgctacgac cgcgccgtgg gattgagtcc gcagattgtg cggctgcggc
     2761 cggccccgca ctgccgaacg cgcattctca gctactcgct caaggtgcag cctcaagatc
     2821 acttcatcaa ttggcagcaa gacccgcagg cgaactattt ggctcggctg gtatttccgg
     2881 acgagacgcg cgaactgaaa atcgaagtcg atctggtcgc ggagatgtcg gtgctcaatc
     2941 cattcgattt ctttctcgcg ccctacgcag agcaatttcc cttcaaatac gattcgaccg
     3001 agcgccgaga attggtgcct tacttggtga agtcgccgtt gagtcagccg ctcgagagtt
     3061 atctcggcgg gatatcccgt gagcgttgcc gcaccatcga tttcgtcgtc ggcgtcaacc
     3121 agcgcgtggc caacgacatt cgctacctga ttcgtctcga gccgggcgtg caatcacccg
     3181 aggagactct caccaggagc gcgggatcgt gccgcgactc ggctgcacta ttggtccaat
     3241 tgatgcgcca tgtggggctc gccgcgcgct tcgtctcggg ctatctcgtg caactggtgg
     3301 cggacgtcaa ggcactcgac gggccggtcg gcgcgacgca ggactttacc gatttgcatg
     3361 cctggtgcga agtgtacctg ccgggcgcgg gctggatcgg gctcgatccg acctccggat
     3421 tgctcgccgg cgaaggacac attcctctgg cctgtacgcc ggaaccctcg gcggcagctc
     3481 ccgtgagcgg tgcggtcgat gagtgcgaga cgacgttcga acaccgcatg agtgtgcgac
     3541 gcatttggga ggcgccgcgt gtcaccaagc cttacagcga tgcccaatgg agctcgatcg
     3601 aggcgctcgg ccgcgctgtc gacgcggacc tggagagtca ggatgtgcgc ctcaccatgg
     3661 gcggcgagcc gacgttcgta tccagcgacg atccggatgg cgaggaatgg aacagcgcgg
     3721 cgcttggcaa gaacaaacgc cggctggccg cggacatcta tcggcggctc aagaacaaat
     3781 atgcccccgc aggtcttgcg cacttcggtc aaggcaaatg gtatccaggc gagcaactgc
     3841 cgcgctggtc cttgaattgc tactggcgca aggacggcga gccgttatgg acgaagccgc
     3901 agctgctcgc cgacgagcaa gccccgcagg cgctgacggc ggaatccgcg cggcgcttcc
     3961 tgacacgagt cgctgagacc ttggcgctcg atccgaagtt cgtattcccg gcttacgaag
     4021 acgcatttta ttatctgtgg cgcgaacggc gtctgccggc caacgtcgat ccgttcgagt
     4081 cgcgattgca agatcctatg gagcgcgagc gcctggcgaa gatcttcgaa cagggcatgg
     4141 ggcagacgat cggctatgtg ctgcctatcg cgcgcaccgc cgaggatgag cgatggcgca
     4201 gcggagcctg gtttctgcgc agcgagcgct gctacttgat ccccggcgat tcgccgatcg
     4261 gctaccggtt gccgctcgag tcgcagccgt gggtggcggc cggggacttt ccgttcattc
     4321 acccgccgga tccgtcgcgc agcttccctg cgctggcagc gcacgccgag attcgccggc
     4381 aattccgcga atccgcggcg gtgcccctca atgaaactgc gccggcggcg caagaatccg
     4441 ccgcggcgat cgcgcgcacc tccatgtgcg ccgagccgcg caatggcgtg ctgtatatct
     4501 tcatgccgcc ggtcgccgaa ctcgaacact atctggaact ggtggcggcg gtcgaagcca
     4561 cggcggaggc cctggcgcaa cccgtcatcc tcgaaggcta tgaaccgcct aaggatccgc
     4621 gactcggcag ctttcgcgtc acgccggacc ccggtgtgat cgaggtcaat gtacatc
//
LOCUS       DASWZT010000012         6197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_19475, whole genome shotgun sequence.
ACCESSION   DASWZT010000012 DASWZT010000000
VERSION     DASWZT010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6197
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19475"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="VGE92_00210"
     CDS             <1..797
                     /locus_tag="VGE92_00210"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY1312263.1"
                     /translation="HAGGEAARVAELLEGAKRRHAEQMVQLTQLKNDQAAQITRLKAE
                     ADTHEQEMGVLMAHLKEARRPIESIEADVRRLTEELAAKSAAHAHAEEENRKLSATLE
                     RTRGALEEREFLIRRLERSESNNANVLGRIQTSIERLGAVPMGASTITGASPEFSAEL
                     IRVDGDRPVTHVLSRRTRIGRAAGCELQIESNSVSRHHAMVLVGPREAIIEDLNSTNG
                     VVVNGRKVSRHLLSDGDAVAIGDIQFRYLARVLPRVVAATAVESNA"
     gene            843..917
                     /locus_tag="VGE92_00215"
     tRNA            843..917
                     /locus_tag="VGE92_00215"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:875..877,aa:Val,seq:gac)
     gene            948..2867
                     /gene="thrS"
                     /locus_tag="VGE92_00220"
     CDS             948..2867
                     /gene="thrS"
                     /locus_tag="VGE92_00220"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017737823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEY1312264.1"
                     /translation="MPVITLPDGSEKRFDRAVSVADVAASIGPGLAKAALAGKLDGVL
                     VDTSKLIDHDASLSIVTDKDAAGLEIIRHSTAHLLANAVQELFPDAQVTIGPVIEDGF
                     YYDFAYKRPFSTEDLAAIEKRMAEISAKDLKVHRRVLPRDEAVAHFRKIGEHYKAEII
                     GAIPAGESISLYGQGDWEDLCRGPHVPSTGKLKAFKLMKVAGAYWRGDSRNEMLQRIY
                     GTAWANEKQLKEYLTRLEEAEKRDHRRIGKELDLFHLQEEAPGAVFWHPKGWALFQQL
                     IAYMRERQTAAGFQEVNTPEVMDRELWVKSGHIETFGENMYMTQTPDERIFALKPMNC
                     PGHIQVFRQGLRSYRELPLRFAEFGKVHRYEPSGALHGLMRVRAFTQDDAHIFCTPEQ
                     ITTEAVKVTELILSIYRDFGFDDVRIKFSDRPAKRVGSDLIWDQAEAALRQAAAAAGI
                     ETTLNSGEGAFYGPKLEFVLRDAIGRDWQCGTLQVDFNLPGRLGAAYVGEDSQKHVPV
                     MLHRAMFGSLERFIGILIEHHAGKLPTWLAPVQAVVLGITDRQDKYCLEVTENLKNQG
                     LRVETDLRYEKVGFKIREHTIKRVPFLLVAGDREVEGRTLSVRTRAGEDLGTMELSKL
                     YSFLRDDVASRGHTV"
     gene            2879..3397
                     /gene="infC"
                     /locus_tag="VGE92_00225"
     CDS             2879..3397
                     /gene="infC"
                     /locus_tag="VGE92_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEY1312265.1"
                     /translation="MATSKRVRRNEEISAPSVRVIAADGSQAGVMTRAEALQMAVSQT
                     LDLVEVSPMAEPPVVRVMDFGKFLFEQNKKAHAAKRKQKQIQVKEVKFRPGTEEADYQ
                     VKLRSIIRFLTEGDKAKVTLRYRGRELVHQDIGRRLLTRISEDLAPYSVVEQNPLMEG
                     KQMVMVFGPKKK"
     gene            3483..3677
                     /gene="rpmI"
                     /locus_tag="VGE92_00230"
     CDS             3483..3677
                     /gene="rpmI"
                     /locus_tag="VGE92_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEY1312266.1"
                     /translation="MPKLKTKRAAAKRFRKTANGFKRGQSHRRHILTKKPSKRKRQLR
                     REMHVDKTDVKRIKQCLPNA"
     gene            3696..4055
                     /gene="rplT"
                     /locus_tag="VGE92_00235"
     CDS             3696..4055
                     /gene="rplT"
                     /locus_tag="VGE92_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEY1312267.1"
                     /translation="MARVKRGVVAGRRHKKILKKAKGYFNARRKVFRTAKQAVIKAGQ
                     YAYRGRREKKREFRALWITRINAASRLCGMTYSTLIDGLNKAGLAIDRKVLADLAVHD
                     MPAFTAIAEKAKASLAA"
     gene            4060..5079
                     /gene="pheS"
                     /locus_tag="VGE92_00240"
     CDS             4060..5079
                     /gene="pheS"
                     /locus_tag="VGE92_00240"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEY1312268.1"
                     /translation="MTDSLSALVEQALADVNASANLVALDEVRVRLLGKKGLLTEQLK
                     GLGTLSAAARPAAGQRINDAKTSIHAALEMQRIRLEQAALEQELAKGTLDVTLPGRGQ
                     ESGSLHPITRTRLRIERIFSQAGFQIATGPEVEDDFHNFEALNIPKNHPARAMHDTFY
                     FPDGRLLRTHTSPVQIRSMRAQSPPLAVIAPGRVYRKDSDMTHTPMFHQAEGIVVGEN
                     ISFANLKAMLHTFVERYFEQALGMRLRPSYFPFTEPSAEVDIECVFCRGAGCRVCKQT
                     GWLEILGCGMIHPNVLAAAGIDSERWQGYAFGMGIERLAMLRYGVDDLRLFFENDLQF
                     LKQFP"
     gene            5076..>6197
                     /gene="pheT"
                     /locus_tag="VGE92_00245"
     CDS             5076..>6197
                     /gene="pheT"
                     /locus_tag="VGE92_00245"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY1312269.1"
                     /translation="MKVTYSWLREYVPLAPPAELAKQLTLAGLEVESVQSAAPAFSGV
                     VVGEVLESGRHPDAEKLSLCLVTTDGSNRLQIVCGAPNVRAGLKVAVAMVGARLPNQV
                     LIKRATLRGVESNGMLCSARELGLGSEHDGIMELAPALPLNADLRDALDLDDVMMEVN
                     ATPNRGDCMSVFGIARDYAAAQTRHYLALQIAPVAAQGADTLPVTLEAPAACPVFASR
                     VIRGVKVGAASPAWLRERLRRVGINSISPIVDVTNYVMMDLGQPMHAYDLAKIDQGIR
                     VRMAKSGERLTLLDDQEYALDPEMLVIADAAGPIGLAGIMGGRATAISDATRDVLLEA
                     AHFAPDAIAGRARRLGLFTDAAQRFERGVDPSLPALAIER"
BASE COUNT         1270 a         1784 c         2014 g         1129 t
ORIGIN      
        1 agcatgccgg cggcgaggcg gcacgcgtgg ccgaattgct ggagggcgcg aagcgccgcc
       61 acgccgaaca aatggtgcag ctgacgcagc tgaagaacga tcaggcggcg cagatcacgc
      121 gcttgaaagc cgaggccgac acgcatgagc aggaaatggg cgtgctcatg gcgcacttga
      181 aagaggcgcg tcgaccgatc gaatcaatcg aagccgacgt caggcggctc accgaggagt
      241 tggccgcgaa gagcgctgcg cacgcgcacg ccgaagagga gaatcgcaaa ctctctgcga
      301 cgttggagcg aactcgcggt gccctggaag agcgcgaatt cctcattcgc cgcttggagc
      361 gcagcgaaag caacaacgcc aacgtgctcg gacgcattca aaccagcatc gaaagactgg
      421 gagcggtgcc gatgggcgcg agcacgatta ccggcgccag ccccgagttc tcggccgagc
      481 tgatccgagt cgatggcgac cggccggtga cgcacgtgtt gtcgcgccgc acgcgtatcg
      541 gccgtgccgc cggctgcgaa ttgcagatcg aatcgaactc ggtgagccgg caccatgcga
      601 tggtgttggt cggaccgcgg gaggccatca tcgaggatct caatagcacc aacggcgtgg
      661 tggtgaacgg ccgcaaggtg tcgcgccacc tcttgagcga tggcgacgcg gtggcgatcg
      721 gcgacatcca attccgctat ctcgcccggg tcctgccgcg cgtggttgcg gcgactgccg
      781 tggagtccaa tgcttaaggg gtgatttttc gggcgaaatc cggtatcttt cgcgccctcc
      841 gagcgcgctt agctcagcgg tagagcatca ccttgacatg gtgggggtca gtggttcgat
      901 cccactagcg cgcaccatcc ttgtgtgtcc cttggtaaga tgccgcaatg cctgtaatta
      961 ctcttccgga cggaagcgag aaacgctttg atcgcgcggt ttccgtggcg gacgttgctg
     1021 caagcatcgg gcccggtctg gccaaggcgg cgctcgccgg caagctcgac ggcgtgctgg
     1081 tcgatacctc gaagctcatc gatcacgacg caagcttgag catcgtgacc gacaaagacg
     1141 ccgctggcct ggaaatcatc cgccactcga ccgcgcacct cttggcgaat gcggtccagg
     1201 agttgtttcc cgatgcccag gtcacaatag gccccgtcat cgaggacggc ttctactacg
     1261 attttgccta taagcggccg ttctcgaccg aggatctggc ggcgatcgag aagcgcatgg
     1321 cggagatctc cgccaaggat ttgaaggtgc atcgacgcgt gctgccgcgc gacgaagcgg
     1381 tggcgcactt taggaaaatc ggcgagcact acaaggcgga gatcatcggc gcgattccgg
     1441 cgggcgagtc gatcagcctg tacgggcagg gcgattggga agacctgtgc cgcggaccgc
     1501 atgtacccag caccggcaag ctcaaggcct tcaaactgat gaaggtggcg ggcgcctact
     1561 ggcgcggcga ttcgcgcaac gaaatgttgc agcggatcta cggcaccgcc tgggccaacg
     1621 agaagcaact caaggagtac ctgacacggt tggaagaggc cgagaagcgc gatcatcgac
     1681 gcatcggcaa ggaactcgat ctgttccacc tgcaggagga ggcgcccggt gcggtgtttt
     1741 ggcacccgaa aggatgggcg ctctttcagc agctgatcgc ctatatgcgc gagcggcaga
     1801 cggcggcggg cttccaagaa gtcaacaccc ccgaggtcat ggatcgagag ctgtgggtga
     1861 aatccggcca catcgagacg tttggcgaga acatgtacat gacgcagacg ccggacgagc
     1921 gcatctttgc gctcaagccg atgaactgtc cggggcacat ccaggtgttc aggcagggcc
     1981 tgcgcagcta tcgcgagttg ccgctgcgtt tcgccgagtt tggcaaggtg caccgctacg
     2041 aaccatcggg tgccctgcat ggcctgatgc gggtccgcgc cttcacgcag gacgacgcgc
     2101 atatattttg caccccggag cagatcacca ccgaggcggt caaggtcacg gagttgatct
     2161 tgagcatcta ccgcgatttc ggtttcgacg acgtgcgcat caagttctcc gaccggccgg
     2221 ccaagcgggt cggttccgat ctgatttggg accaggccga ggcggcgctg cgccaggcgg
     2281 ccgcggccgc cggcatcgaa acgaccttga attcgggcga aggggcattt tatggcccca
     2341 aactcgagtt cgtgctgcgc gacgcgatcg ggcgcgattg gcaatgcggc accctgcagg
     2401 tcgatttcaa tctccccgga cgcttaggcg cggcctacgt gggcgaagac agccaaaagc
     2461 atgtgccagt catgctgcac cgggccatgt tcggctcttt ggagcgattt atcgggattt
     2521 tgatcgaaca ccatgccggc aaattgccga cttggctggc cccggtgcag gcggtggtgc
     2581 tcggaattac cgatcggcag gacaaatatt gcctggaagt aacggaaaac ctgaaaaatc
     2641 aggggcttcg ggtagaaacg gacttgaggt atgagaaagt aggctttaaa atccgtgagc
     2701 atacaattaa gcgtgttcct ttcctcttgg tcgctggcga ccgggaagtc gaggggcgca
     2761 ccctgtccgt acgcacgcgc gctggcgaag acctaggcac catggagctg tcgaagctct
     2821 attccttcct tcgcgatgac gtcgcgagcc gcgggcacac tgtttaggag gatcgtgtat
     2881 cgcaacgtcg aagcgggtta ggcgcaatga ggagatctcc gcaccatcgg tgcgcgtgat
     2941 cgcagcggat ggaagccaag caggggtcat gacgcgcgcc gaagcgctgc aaatggcggt
     3001 ctcgcagact ctggacctcg tcgaggtctc gccgatggcg gagccgccgg tggtgcgagt
     3061 catggatttc ggcaagttct tgttcgaaca aaacaagaaa gcgcatgccg cgaagcgcaa
     3121 gcagaagcag atacaagtta aggaagtgaa atttcgtccc gggacggaag aggcggacta
     3181 ccaggtcaaa ctgcgtagca tcatccgctt cctgaccgag ggtgataagg ccaaggtgac
     3241 gttgcgttac cgcggccgtg agctcgtaca ccaggacata ggccggcgtc tactgacgcg
     3301 gatttctgag gatctcgccc cgtattcggt ggtggaacag aatccgttga tggagggcaa
     3361 gcagatggtc atggttttcg ggcccaaaaa gaagtgagcg gcagaccgta acaggttcgc
     3421 cgtccccgcc tgtagcggcg tcgcaagacg cttgtgaaca gggctaaaat taggagtcga
     3481 gcatgcccaa gttgaaaact aaacgggctg cggcgaagcg ttttcgcaaa acagcgaatg
     3541 gcttcaaacg cggtcagtct caccgtcgtc atatcctgac gaaaaaaccc agcaagcgta
     3601 agcgccagct gcgccgcgag atgcacgtcg acaaaaccga cgtaaaacgc atcaagcagt
     3661 gcttgccgaa cgcctaatca gtcaggagaa agaacatggc acgagtcaaa cgcggtgtcg
     3721 tcgcaggacg acgccataag aaaattctca agaaggcgaa gggctatttc aacgcccgtc
     3781 gcaaagtgtt ccgtaccgcc aagcaggcgg tcatcaaggc cggccagtat gcctaccgcg
     3841 gccggcgcga gaagaagcgc gagttccgcg cactgtggat tacgcgcatc aacgcggctt
     3901 cgcgtttgtg cggcatgacc tacagcacgc tgatcgacgg ccttaacaag gcgggtctcg
     3961 ccatcgatcg caaggtgctc gccgatcttg cggttcatga catgccggcc tttacggcca
     4021 tcgcggagaa agccaaggcg agtctcgcgg cctgaagccg tgacggattc cttaagcgcc
     4081 ttggtcgagc aagcgctcgc agacgtgaat gcgtctgcga atctcgtcgc gttggacgaa
     4141 gttcgcgtgc gactgttggg gaagaaagga ctcttgaccg agcagctcaa gggtttggga
     4201 accctgtcgg ccgccgcacg acccgccgcg ggccagcgaa tcaatgacgc gaagacgtcc
     4261 atccacgcgg cgctcgagat gcagcgcatc cgtctcgagc aggcggcgct cgagcaggaa
     4321 ctcgccaagg gcacgctcga tgtgaccctg ccgggtcgcg gtcaagaatc cggcagcctg
     4381 catcccatca cgcgcacgcg attgcgaatc gagcgcattt tctcgcaggc cggatttcag
     4441 atcgccaccg gacctgaggt cgaagacgat tttcacaatt tcgaggcgct gaatattccg
     4501 aagaatcatc cggcgcgggc gatgcacgat acattttatt ttcccgatgg tcggctgctg
     4561 cgcacccaca cctcgccggt acagatcagg agcatgcgtg cgcaatcacc gccgctcgcg
     4621 gtgatcgcgc ctggtcgtgt ctaccgcaag gactcggaca tgacgcatac accgatgttt
     4681 catcaggccg agggtatcgt cgtcggggag aacatcagct ttgccaacct gaaggcgatg
     4741 ctgcacacct ttgtggaacg ctacttcgaa caggctttgg gaatgcgctt gcgtccttcg
     4801 tactttccgt tcaccgagcc atccgccgaa gtcgacatcg aatgcgtgtt ctgccgcgga
     4861 gcgggttgcc gcgtctgtaa gcaaaccggg tggctcgaga tcttgggctg cgggatgatt
     4921 catccgaatg tacttgcggc ggcgggtatc gatagcgagc ggtggcaggg ctatgccttc
     4981 ggcatgggca tcgaacggct cgccatgctg cgttacggcg tagatgatct gcgtctattc
     5041 tttgaaaacg atctgcagtt cctgaagcag tttccatgaa agttacctac tcatggctgc
     5101 gggagtacgt gccgctcgcg ccgccggcgg aactcgccaa gcagctgacc cttgccgggc
     5161 tcgaggtgga gtcggtgcag agtgcggcgc cggcgttctc gggcgtcgtg gtgggcgagg
     5221 tactcgagtc ggggcgccat ccggatgcgg agaaattgtc gctctgtctg gtgaccaccg
     5281 acggatcgaa tcggctgcag atcgtatgcg gcgcaccgaa cgtgcgcgcc ggtcttaagg
     5341 tcgcagtggc gatggtgggc gcgcgtctac cgaaccaggt gctcatcaag cgcgccacgc
     5401 tgcgcggtgt ggaatcgaac ggcatgctgt gctcggcgcg tgagctcggg ttgggcagcg
     5461 agcatgatgg aattatggag ctggcgccgg cgctgccatt gaatgcggat ctgcgagacg
     5521 cgctcgattt ggatgatgtg atgatggagg tcaatgcaac gccgaatcgc ggcgactgca
     5581 tgagcgtgtt cggcattgcc cgcgactacg ctgcggcgca gacgcggcat tacctggctt
     5641 tgcagatcgc gccggtcgcg gcgcaaggcg ccgacacgtt gccggtgaca ttggaagcgc
     5701 cggcagcctg cccggtgttt gcttcgcggg tgattcgcgg cgtcaaagtc ggcgcggcat
     5761 ctcccgcatg gctgcgcgaa cgcctgcgcc gggtcggtat caatagcatt tcgcccatcg
     5821 tcgatgtcac gaattacgtg atgatggatc tgggacaacc catgcatgcc tacgatctgg
     5881 caaagattga tcaaggcatc cgcgtgagaa tggcgaagag cggtgagcgc ctgacgctgc
     5941 tcgacgatca ggaatacgcg ctcgatcccg agatgttggt cattgccgat gccgcgggac
     6001 ccatcggact tgcgggcatc atgggtgggc gcgccacggc aatctccgat gcgacccgcg
     6061 atgtactgct cgaagcggcc cacttcgcgc ccgacgcgat cgccggacga gcgcggcgac
     6121 tcggcctgtt caccgacgcc gcacagcgct tcgaacgcgg cgtggacccg agcttgccgg
     6181 cgctcgcgat cgaacgc
//
LOCUS       DASWZT010000013         7636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_82409, whole genome shotgun sequence.
ACCESSION   DASWZT010000013 DASWZT010000000
VERSION     DASWZT010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7636
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82409"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VGE92_00250"
     CDS             <1..891
                     /locus_tag="VGE92_00250"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1312270.1"
                     /translation="IGFFALENSRFLSLRNLTNILTEVSIYGIIAVGMTFVILTAGVD
                     LAVGSLLAFAGMSGAYVMRALGADYSLSWLVALSTATAVGALVGYLQGKAVTLFNIPP
                     FIVTLGGLTIWRGATLILNDGSPIAGFDASYRWWGTGAIVGIPVPVVIFGVIVAVGLI
                     TQRYTRYGRYIYAVGGNPEAARLNGLDVPAIITSVYLIVGALAGLAGFLLSARLGAAE
                     AVGGVGYELRVIASVVIGGASLAGGRGGIGGSVIGALLIGVLTNGLVIMHVSSYWQQI
                     VIGLIIVAAVAFDTYAKSRR"
     gene            932..3268
                     /locus_tag="VGE92_00255"
     CDS             932..3268
                     /locus_tag="VGE92_00255"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY1312271.1"
                     /translation="MPATARAAAMWITHPDAGTTAPIVIDFRRALELTRPAATYPVRV
                     SADNRFVLYVNGKRVGTGPARSDLAHWRFEQFDLRPLLVRGHNVISAVVWNFVKPETP
                     PLAGQTESERMAAVYRAAREQTGPAAQISAHTGFWLSAVNADDAAIDSDGQWQASIDS
                     RHTAEPAMAHIKRGYYVAGAAEFIDARQPTSAWTAAVPALAEGQASPWQLSADPLPPM
                     DYRATSSGRVTRTDLKDGRRFPAHAVLVPAHTHVHLLLQRDTLVSAYPELLTAGGRDA
                     QITLTYAEALYDSANKKGIRDAVDARQAWGLTDRFIADGTPHAWAPCWWRTWRFLDIE
                     VQTAGEPLTLKGLHTFETGYPFVAIGRFDSDDAGLNDIWRIGWRTARIDAHETYMDSS
                     YWEQLQYVGDTRLQMLISYAVSGDTRLAVQAVDAFAASAADHGLTQGAYPSRNDNVIP
                     PFSLLWIGMLHDYWWQQPDSAVVLRSLARMREILAWYANYLQADGLLRKNPHWNFIDW
                     AVKDGGPLAQGAFPSFDANGESCLTSLVYVGALAQAADLERALGDAALGDSDAQLAER
                     VREAIRRSCWDEARGLFADDPAKQTFSQHANALAVLYDVATPNESSAILARITSAGVD
                     APPGVAGSSYYFAWYLIRAYEHAGLADRYLDLLSTWRDLLKLKFTTWPEQRGNSRSDS
                     HAWSAHPTADLLAIVAGIEPAQPGYQRVRIAPHLGGLTRLTAVAATPAGPIEVRYRVR
                     AGKLDVRIHKPASLGADFLWHGGRHELLGSDTTLTLDR"
     gene            complement(3277..4737)
                     /locus_tag="VGE92_00260"
     CDS             complement(3277..4737)
                     /locus_tag="VGE92_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEY1312272.1"
                     /translation="MVATNDDVLRNCDRRSIFTGPLKVAAAQAISYLSNLDAGPVGAT
                     ADAAVLRTRIAKPLSRCGLPAEQVISELAAAVEGGLHGVASARFFAWVIGGSLPAALG
                     ADWLTSAWDQNAHNYGTGPAAAITEEVVGDWLKELFGLPRQSSFALVTGCQMAHFTCL
                     ASARHALLAKRAWNVEANGMSGAPAIRVLSSDQAHGSIDRAVSMLGIGTSHIFKLPSD
                     SSGRLEPGALVAALAAEPNAPTVVVLQAGDLNIGAFDQFEILIPIAKRHGAWVHIDGA
                     FGLWVAASSSYRHLLAGVEAADSWATDGHKWLNVPYDCGYALVADPTAHGGSMSHRAS
                     YISYSSDARDQADWNPEWSRRARGFSSYAAIRQLGVDGISDMIERCCTQARSLVAEIA
                     HLRGAELVWEPQINQGLVRFLDLNAGASQQDHDRRTDEMIAKINAAGEAFFSGTTWRG
                     RRAMRVSVCNWQTTMSDVTRTVANIARLLHCSPERA"
     gene            complement(4868..5308)
                     /locus_tag="VGE92_00265"
     CDS             complement(4868..5308)
                     /locus_tag="VGE92_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY1312273.1"
                     /translation="MLSQAQLARHLRGHVGAQLYDESAVAPIGIAIYSLSDPRDIREV
                     RYVGQSADPRRRFLQHLNAAKLWLPDERPWWVKSPKLRPLYHWIRELFREELRLPTLV
                     VSAWVATAAEARLAERTRIFDCLARRLPLLNVETEILGRQRALL"
     gene            5457..5984
                     /locus_tag="VGE92_00270"
     CDS             5457..5984
                     /locus_tag="VGE92_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY1312274.1"
                     /translation="MSVTSSFKNATNAVLKAIAQDTDATPETDLLDTLKKEHDEVKDL
                     LSDLQTATTAAQRKSLVNRIKTALVPHTKAEEKILYDAVIASKDKNAQIDGHEGYLEH
                     ECASKILTRLTQIENATSPEHKAAAKVLKELVEHHIDEEERNVWADAKKQFSEEKREQ
                     MNVAYLAAKQKVRIP"
     gene            complement(5995..7419)
                     /locus_tag="VGE92_00275"
     CDS             complement(5995..7419)
                     /locus_tag="VGE92_00275"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY1312275.1"
                     /translation="MLLLGYACAASAQNAAPAPPQSTDERVDALTREVQELKALVLHL
                     QEQLKQVTGTPPSATAGVPAPAAPPAAVPATPAAAAAATPVPSAAPTPTAGASASAAA
                     GILQGATINVLLDGYYEWNSDSPVGRVNSLRAYDVSSNSFSLNQAAVILESAADFAAD
                     KRWGARLDLQFGQATSTLQGSSANELRPDVYRNVFQAYGTYIFPVAQGLTVDFGKWAS
                     SLGMEGNYTKDQLNYSRSFLFDFLPFYHTGLRSKLAINDQLAFNVWLTNGTEQTEAFN
                     NEKDTMVGLVLTPIPAISWTLNYYRGQEHPDVIYEQTPTQPNLPNEQGTYILPIVNAP
                     NGALDIFDSYVTWQATTNLQFAAEADYVQQRLYTYSSPARVEAGAFYVGYQFYPTLTF
                     AARAEYLADIGGLFSGTTQYLKEGTLTLDYRPSDHFLMRGEYRRDQSNRLYFLGHTLD
                     VFESIQPTLGLGLVWWFGQKQGVW"
BASE COUNT         1443 a         2416 c         2385 g         1392 t
ORIGIN      
        1 atcggctttt tcgcgctgga gaactcgcgc tttctgtcgc tgcgcaacct caccaatatc
       61 ctcaccgaag tgtcgatcta cggcatcatc gcggtgggga tgacgttcgt cattttgacg
      121 gcgggcgtcg acctcgccgt cggctcgctc ctggcgttcg cggggatgtc gggggcctac
      181 gtcatgcgcg ccctcggggc ggactattcc ttgagctggt tggtggccct atcgacggcc
      241 acggcagtgg gggcgctggt cggttaccta caaggcaagg cggtgacgct ctttaatatt
      301 ccaccgttta tcgtgaccct gggagggctc acaatctggc gcggcgccac gctcattctg
      361 aacgacggct cgcccatcgc ggggttcgat gcgagttatc gctggtgggg gaccggcgcg
      421 atcgtcggca tcccggtgcc ggtggtgatt ttcggcgtca tcgtcgccgt gggcttgatc
      481 acccagcgct acacgcgcta cggacgctac atctacgcgg tcggcggaaa tccggaggcg
      541 gcccgcctca acgggctcga cgtgccggcc atcatcacca gtgtctactt gatcgtcggc
      601 gcgctcgccg gcctcgccgg atttctctta tccgcccggc tcggcgccgc cgaagcggtc
      661 ggcggcgtgg gctatgagct gcgcgtcatc gcctcggtgg tgattggcgg cgcgagcctc
      721 gcgggcggcc gcggcggcat cggcggcagc gtcatcggcg cgctgttgat cggcgtgctc
      781 acgaacggct tggtgatcat gcatgtctcc tcgtattggc agcaaatcgt gatcggactg
      841 atcatcgtcg cggcggtggc attcgacacc tatgcgaagt cgcgccgcta gcgcgagcgc
      901 ctgggcctcg gccgcatgca cgctcgccgt catgccggca acggctcgcg ccgcggcgat
      961 gtggattacg cacccggacg ccggcaccac ggcacccatc gtgatcgatt tcagacgcgc
     1021 tttggaactc acgcgcccgg cagcgacgta tccggtgcga gtcagcgccg ataatcgatt
     1081 cgtgctgtac gtcaacggca agcgcgtcgg cacgggcccg gcgaggagcg acctcgcgca
     1141 ttggcgtttc gaacaattcg acttaaggcc gctgctggtg cgcgggcaca atgtcatttc
     1201 cgcggtggtg tggaatttcg tcaaacctga aacgccgccg ctggcaggcc aaaccgaatc
     1261 cgagcggatg gcggccgtgt accgggcggc gcgcgagcaa accggcccgg cggcgcagat
     1321 ctcggcgcac accggcttct ggttgagcgc cgtcaacgcc gatgacgcgg cgattgactc
     1381 cgacgggcag tggcaggcgt caatcgactc gcgccacacg gcggaacccg ccatggcgca
     1441 catcaagcgc ggctactatg tggccggcgc cgccgagttc atcgatgcac ggcagcccac
     1501 gtccgcctgg acggcggcgg tacccgcgct cgccgaaggg caggcgtcgc cctggcaatt
     1561 gtcggcagat ccgctgccgc cgatggacta tcgggcaacc tccagcggcc gagtgacgcg
     1621 cacggatctt aaagacggcc ggcgctttcc cgcccatgcg gtgctggtgc ccgcgcacac
     1681 gcacgttcat ttactgctgc aacgcgacac gctggtctcc gcctacccgg aacttctgac
     1741 cgcgggcggg cgcgatgccc agatcacgct cacctatgcc gaggccttgt acgacagcgc
     1801 caataaaaaa ggcatccgcg atgccgtcga cgctcgtcaa gcctgggggc tcacggatcg
     1861 cttcatcgcg gatggcactc ctcacgcatg ggctccctgc tggtggcgta cctggcggtt
     1921 cctggatatc gaggtacaaa ccgcgggcga gcccttgaca ctcaagggcc tgcatacctt
     1981 cgagaccggc tatccgttcg tcgccatcgg gcgcttcgac tcggatgatg ccggactcaa
     2041 tgatatctgg cgcatcggct ggcgcaccgc gcgcatcgat gcccatgaga cctacatgga
     2101 ttcatcgtac tgggagcagc tgcaatatgt cggcgatacg cggctgcaga tgttgatttc
     2161 ctatgccgtc tcgggcgata cgcgcctcgc cgtgcaagcc gtggatgcat tcgccgcctc
     2221 ggcggccgat cacggtttga cgcaaggcgc gtatcccagc cgcaacgaca acgtcattcc
     2281 gccgttctcc ttgctctgga tcggcatgct gcatgattat tggtggcaac agccggacag
     2341 cgcggtagtg ctgcgcagtc ttgcgcgcat gcgcgagatt ttggcctggt atgcgaacta
     2401 cctgcaggcc gatggattat tgcgcaagaa tccgcattgg aatttcatcg actgggcggt
     2461 gaaggacggc ggcccgctcg cccagggcgc gtttccatcg ttcgacgcca acggcgagtc
     2521 gtgtctcacc agtttggtgt atgtcggggc gctcgcccag gccgcagacc tcgaacgcgc
     2581 gctcggtgat gcggccctgg gggacagcga tgcgcagctc gcagagcgcg tgcgcgaggc
     2641 gattcgccgt tcctgctggg acgaggcgcg cggcttgttc gccgacgatc cggccaagca
     2701 gaccttcagt cagcacgcaa atgcgctcgc cgtgctctac gatgtggcga cccccaacga
     2761 gagcagcgcc atactcgcac gcatcacgag cgcgggagtc gatgcgccgc ccggggtcgc
     2821 gggctcgagt tactattttg cgtggtactt gatccgcgcc tacgagcacg cgggactcgc
     2881 ggatcgctac ctcgacctct tgtcgacgtg gcgagacttg ctcaagttga aattcacgac
     2941 gtggcccgag cagcgcggca attcgcgctc ggacagtcac gcctggagcg ctcatccgac
     3001 cgccgatttg ctggccatcg tcgccggcat cgagccggca caacccggct atcagcgcgt
     3061 gcgaatcgcg ccgcacttgg ggggattgac gcgcctgacg gcggtcgcgg cgacgcccgc
     3121 gggcccgatc gaggttcgct atcgggtgcg cgccgggaag ctcgacgtgc ggatccacaa
     3181 gcccgcctcg ctgggcgctg atttcctgtg gcacggcggc cgccacgaac tcttgggcag
     3241 cgataccaca ctgacgctcg atcgataggc gcgtacttac gcccgctcag gcgagcaatg
     3301 cagcagccga gcaatattcg ccaccgtgcg cgtgacatcc gacatcgtcg tttgccaatt
     3361 gcacacgctc acccgcatcg cgcgccggcc gcgccacgtg gtgccactga agaacgcttc
     3421 acccgcagca ttgatctttg cgatcatctc gtcggttctg cgatcgtggt cctgttgaga
     3481 agcccccgca ttgagatcca agaatcggac caacccttga ttgatctgcg gttcccagac
     3541 caactctgct ccgcggagat gggcaatttc cgccaccaaa gaccgcgcct gcgtgcagca
     3601 gcgctcaatc atgtcggata ttccatcaac acccagttgc ctgatcgctg catagctcga
     3661 aaatccccta gcccgacgcg accactcggg gttccagtca gcctggtcac gcgcatccga
     3721 ggaataagaa atgtatgacg cacgatgcga catcgaaccg ccatgcgctg ttggatcggc
     3781 aaccaaggca tagccgcagt cataaggaac gttgagccat ttatgtccat cggtcgccca
     3841 agagtccgcg gcctcgacac cggcgagcaa gtgccggtaa gacgagctgg ccgcaaccca
     3901 tagtccgaac gctccatcga tatgaaccca tgcgccgtgt cgcttcgcaa tgggaatgag
     3961 gatctcaaat tggtcgaatg cgccgatatt cagatcgccg gcctgcaaga cgacaaccgt
     4021 cggcgcgttg ggctcggcgg ccagtgccgc tacgagcgcg ccaggctcga gtcggccgct
     4081 cgagtcagag ggtaacttga aaatatggga tgtaccgata ccgagcatac tcacggcgcg
     4141 atcgattgaa ccatgagctt ggtcgctgga cagaacccgg atggccggcg caccggacat
     4201 tccattcgct tcgacattcc atgcgcgctt cgcgagcaac gcatgccgcg ccgacgccag
     4261 gcaggtgaaa tgagccatct ggcagccagt caccagtgcg aatgagcttt gccgcggcag
     4321 gccgaatagc tccttcaacc aatccccgac gacttcctcc gtgatggccg ctgccggtcc
     4381 cgtgccgtag ttgtgcgcat tttgatccca tgcggaggtg agccaatcgg cgccgagcgc
     4441 ggccggcaga gaaccgccaa tcacccaggc gaaaaagcgt gcgctcgcaa caccgtgcaa
     4501 gccgccctcg acggccgccg ccagttcgct gatcacttgc tccgccggca gtccgcagcg
     4561 cgaaagcggc ttggcaattc gagttcgcag caccgcggca tccgccgtcg cgcctaccgg
     4621 gcctgcatcc aagtttgaga ggtaggagat tgcttgcgcc gccgccacct tgagcggccc
     4681 cgtaaagatc gatcgacggt cgcaatttcg aagaacgtcg tcgttggtag ccaccatttc
     4741 cccgctcttt atgactgcgc gcaccgcgcg tcgcggaatt tacaacaaac ggtgatataa
     4801 caccggccgt tttcggactt tgaaaattgt tcgacaacgc tcgcgggtga ataatcagcg
     4861 atgttcgcta caacaaggcc cgttgccggc cgagaatctc ggtttccacg ttgagcaacg
     4921 gcaggcgccg cgccaggcag tcgaaaatcc gggtgcgctc cgcgagcctc gcctccgcgg
     4981 cggtcgccac ccacgcggaa acgaccagcg tcggcaggcg caactcctcc cggaacagct
     5041 ctcggatcca atggtacaag ggacgaagct tgggcgactt cacccaccac ggccgctcgt
     5101 ccggcagcca taatttcgcg gcgttcaggt gctgcagaaa gcggcgccgc ggatccgcgc
     5161 tctgcccgac atagcgcacc tcgcggatgt ctcgcgggtc cgacaaggaa tagatcgcga
     5221 tgccgatcgg cgccacggca gactcatcgt acagttgcgc gcccacgtgg ccgcgaaggt
     5281 ggcgggcgag ttgtgcctgg ctcagcatcg cgatcctaaa agcggttccg ccgaaccgtc
     5341 gcgacggccg ctaggaaaaa tcccacatcc atttgctgtt cctttcactt gtccagacct
     5401 cggtacccgg cgaagcttca cccagccgca atgccgcggc accgagggga ataaccgtga
     5461 gcgtaacgag cagtttcaaa aatgcaacca acgcggtact caaggcgata gcgcaagata
     5521 ccgatgcgac gcccgaaacc gacctgttgg atacgttgaa aaaggagcac gacgaggtca
     5581 aggacctgct ctccgacttg cagacggcga cgaccgccgc gcaacgcaag agcctggtca
     5641 accgcatcaa gacggcgctg gtgccgcata ccaaggccga ggagaaaatt ctctacgatg
     5701 ccgtgattgc atcgaaggac aagaacgcgc agatcgacgg gcacgaggga tacctcgagc
     5761 acgagtgcgc gtcgaaaatc ctgactcgcc tcacccagat cgagaatgcc acctcacccg
     5821 aacacaaggc cgccgccaag gtcttgaagg aattggtcga gcatcacatc gacgaggagg
     5881 agcgcaatgt gtgggccgat gcgaagaaac aattctcgga ggagaaacgc gagcaaatga
     5941 atgtcgcgta cctggccgcg aagcagaaag tgcggatccc ttaaaagcga cgcatcacca
     6001 aacgccctgt ttttgcccaa accaccacac gaggccgagc ccgagcgtgg gttgtatgct
     6061 ttcgaagaca tcgagcgtgt ggcctagaaa atacagccgg ttcgactggt cgcgacgata
     6121 ttcgccgcgc atcaggaaat gatccgaagg ccgatagtcc agagtcagcg tgccttcctt
     6181 gagatattgc gtcgtgccgc tgaacaggcc gccgatgtcg gccaaatatt cggcgcgcgc
     6241 cgcgaacgta agtgttggat agaattgata tcccacgtag aaggcgcccg cctcgacgcg
     6301 cgccggagag gaataggtgt acaaccgctg ctgcacgtag tccgcctcgg cggcgaattg
     6361 caggttggtc gtcgcctgcc acgtgacata gctgtcgaag atgtcgagcg cgccgttcgg
     6421 cgcattgacg atcggcagaa tatacgttcc ctgttcgttc ggcaggttgg gctgcgtcgg
     6481 cgtctgctca tagatgacat cgggatgctc ctggccacga taataattga gcgtccaact
     6541 gatcgcgggt atcggcgtca gcacgagacc caccatcgtg tccttctcgt tgttgaaggc
     6601 ctcggtctgc tcggtgccat tcgtgagcca cacattgaag gcgagctggt cattgatcgc
     6661 caacttcgag cgcaagccgg tgtgataaaa gggcaggaag tcgaacagga aggagcgcga
     6721 atagttgagc tggtccttgg tgtagttgcc ttccatgccg agcgagctcg cccatttgcc
     6781 gaaatcgacg gtcaatcctt gggccacggg gaaaatatac gtgccgtaag cttgaaagac
     6841 gtttcgatag acatcggggc gcaattcatt ggcggagctg ccttgcaggg tcgaggtcgc
     6901 ttgtccgaat tgcaggtcga gccgcgcgcc ccagcgctta tcggccgcaa agtccgcggc
     6961 gctttccaag atcacggccg cttggttgag gctgaagctg ttgctgctca catcgtaggc
     7021 gcgcaaggaa ttcacccgtc ccacgggcga gtcgctgttc cattcgtagt acccatcgag
     7081 aagcacgttg atggttgccc cttggagaat gccggcggcg gcgctcgcgc tcgcgccggc
     7141 ggtgggcgtc ggcgccgcag acgggacggg agtcgctgcc gcggctgcgg cgggcgttgc
     7201 gggcaccgcc gcgggcggcg cggccggcgc gggcacaccg gcagtggccg atggcggggt
     7261 acccgtcact tgtttcagct gctcttgaag atgcagcacc agggctttga gttcttgaac
     7321 ttcgcgcgtg agtgcatcga cgcgctcgtc cgtgctttga ggcggcgccg gcgcggcgtt
     7381 ctgcgcggat gcggcgcaag cgtacccgag cagcagcatc gcgctcgcga tgcgcgcgta
     7441 cttcgcgaaa caagtggtgg aatttgctcg gggcgccgga tcactgttag agtgaaacgc
     7501 tcctggagca accgtacatc ccgttaggct catagcgcat catccttgtt gatccagtcc
     7561 gccaccgaaa atcgcacgga cccatcgtgc tggcaagccg gtcggcaagc caattggcaa
     7621 gccggttggc gagcca
//
LOCUS       DASWZT010000014         4642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_33966, whole genome shotgun sequence.
ACCESSION   DASWZT010000014 DASWZT010000000
VERSION     DASWZT010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33966"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1650)
                     /locus_tag="VGE92_00280"
     CDS             complement(<1..1650)
                     /locus_tag="VGE92_00280"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1312276.1"
                     /translation="MSVVEAALKRPYTVAAMLILISLMGIGAALRMPIDIFPEIDIPV
                     VAVVWTYNGMSARDMQNRILTLHQRQLASLVDDIARIEAVSYEGVGVEKIYLHEGADV
                     TRAVSQLASSALVVLKYMPPNITPPLVLRYGATDVPIIQLSLSSKSLPDTELNDLGQN
                     IIRPSLAVVHGAEVPYPYGGKPRVIMADLDLAALQARGLSASDVSSALQRQNVILPSG
                     DVKIGDKDYALTMNNSPDLIDTINAFPIKQVGGRTVFMRDVAHVHDGFQVQTNSVSVN
                     GTPGALMTIRKTGGVSTLAVISGVRAALKDIRKVLPRDVSIKALFDQSVFVKAALNSV
                     LLSAGMAAMLTAVVIILFLGNVRLSVIILAAIPLSIVSAVLFINLAGQTLNTMTLGGF
                     ALAIGILVDNGTVVIENIERHVAMHEPLHQAIVAGASEVAIPTFLSTLCICIVFVPVF
                     LLQGTAKYLFSPLSLAVIGSLLASLALSFTMVPVLFNFLMRRAVHESEEKSAQACRQR
                     AAHKNPLVRLQAKFESGFEKFRENYRNTLAWTLSEARLTVLA"
     gene            complement(1675..2385)
                     /locus_tag="VGE92_00285"
     CDS             complement(1675..2385)
                     /locus_tag="VGE92_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY1312277.1"
                     /translation="MTLGQAANPAAIRIVIAEDQAIVRRGVALLLAIEPDMEVVGQAS
                     NGVEAVDLTLLLRPDVVLMDLHMPVKGGVAATREITRALPTTQVLVLTTLDDDETVFE
                     AVRAGAHAYLLKDVAEEELLATIRALRRGESHLTPQIARKVMDQFRRLARDQPEARSA
                     ERPPAFDGVASVETLNDKEEKILELIAEGRSNRQIGQTLFLAEGTVKNYVSRIMEKLH
                     ANTRTELAILTHKQRRPD"
     gene            2476..4335
                     /locus_tag="VGE92_00290"
     CDS             2476..4335
                     /locus_tag="VGE92_00290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY1312278.1"
                     /translation="MSNAKPQEPRSLAWDIDPRRSLAAAAMWLIVGLAITFSIAAALW
                     VGSIARANVLEQHVRRLSLETEQLSSDLGQALAARLGAIRAARTLAPNDLEDVFNELV
                     SAYPDLDWIAIADTAGVVVSTRNSLPAGTEIASRRWFAAGLHGPWLGVIDEQPRASGV
                     FGDMAAPVRDQAGRVVGVIAAHLSWQRAPNHPVRLTDEVDPQVATQAYVLDRDGAVLV
                     GPADSRGSHWQGIGAGAASAVPQFERLPNGRRILASRAPLSAAGEVAALGWQALLCEP
                     IERVYQRADALALKILWVSLCLGAATAFLGIIGTRQLTRRLQRLTQSVAFLGQNPSAR
                     LAVPQGLDEVAQLGRAFATIHDDLQRERSELQTLSGELERRVAVRTLEVQRLAEESRY
                     AAIVRERLKIARDLHDTLAHSMMAMLSEVRFLRRLLTHDPAALPAELAHAEELAHDGL
                     KEARGAITQMRVTAVRDMGLGPALAKAFERFIDHTGLAGEFTAQPAAARFGDERAETI
                     FNMANEALRNIERHARATRIVVSLKSVDEMHLELCIEDNGVGFDASAVRPGHYGIVGL
                     REQAELIGAQLRIDSRPEEGTRVCVLWRVSPIEFIGGAGRDAQGASNSSSVLK"
     gene            complement(4299..>4642)
                     /locus_tag="VGE92_00295"
     CDS             complement(4299..>4642)
                     /locus_tag="VGE92_00295"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY1312279.1"
                     /translation="VDGLKRVVEELLRRHRVKYSLEWFVSGEPFLTAPGELSQAATRA
                     VQEQLQITPKLSTGGGTSDGRFIAPLGAQVIEMGVINQTIHKVNECVRVADIDRLQRI
                     YFRTLELLLAP"
BASE COUNT          850 a         1472 c         1461 g          859 t
ORIGIN      
        1 cgccagcacc gtgaggcgcg cctccgacag cgtccacgcc aatgtattgc ggtaattctc
       61 gcgaaatttc tcgaatccgc tctcgaactt cgcctgcaat ctcaccaggg gattcttgtg
      121 agcggcgcgc tgccggcacg cttgcgcgga tttctcctcg gactcgtgca ccgctcggcg
      181 catcaggaaa ttgaacaaca ccggcaccat ggtgaacgac aacgcgagac tcgcgagcag
      241 cgaaccgatc accgcgagcg acaggggcga gaacagatac tttgccgtgc cttgcagcaa
      301 gaatacgggg acgaacacga tgcagatgca caaggtcgac agaaacgtgg gaatcgccac
      361 ttcgctcgcc ccggcaacga tcgcctggtg caggggctca tgcatcgcga cgtggcgttc
      421 gatgttctcg atgaccaccg ttccattgtc caccaagata ccgatcgcca gcgcgaatcc
      481 gccgagcgtc atggtattga gcgtctggcc cgcgagattg atgaacagca cggcgctcac
      541 gatcgacagc gggatggccg ccagaataat taccgacagc cgcacgttgc caagaaacag
      601 aatgatgacc acggccgtca gcatcgcggc catgccggcg ctcaacaaca cgctgttgag
      661 cgccgccttg acgaataccg attgatcgaa cagcgccttg atcgacacgt cgcgcggcaa
      721 cactttcctg atgtctttga gcgccgcgcg gacgcctgag atgacggcga gcgtcgacac
      781 gccgccggtc ttgcgtatcg tcatcagcgc accgggcgtg ccgttcacgg acactgaatt
      841 ggtctgtact tgaaagccgt cgtgcacgtg cgcgacgtcg cgcatgaaca ccgttcggcc
      901 gccgacctgc ttgatgggaa aggcgttgat ggtgtcgatg aggtccggcg aattattcat
      961 ggtcagcgca taatccttgt cgccgatttt tacgtcgccc gacggcaaga tgacattctg
     1021 ccgctgcagt gccgagctca catccgatgc cgacaggccg cgcgcctgca gagcggcaag
     1081 gtcgagatcc gccatgatca cgcgcggctt gccgccgtag ggatacggca cttcggcgcc
     1141 atgcaccacc gcgagcgagg gccggatgat gttctgcccc aggtcgttga gctcggtgtc
     1201 gggcagcgat ttgctggaca gactcaactg aatgatcggc acgtcggtgg cgccgtaacg
     1261 caacaccagc ggcggcgtga tgttgggtgg catgtacttg aggaccacca gagcgctgct
     1321 cgcgagctgc gacacggcac gcgtcacgtc ggcgccctcg tgcagataga tcttctcgac
     1381 gccgacgccc tcgtagctca ccgcctcgat gcgcgcaata tcatccacca gggaggcgag
     1441 ctggcgctga tgcagcgtca gaatgcgatt ttgcatgtcc ctggcgctca taccgttgta
     1501 ggtccacacc accgcgacca ccgggatgtc gatctcaggg aagatgtcga tcggcatgcg
     1561 caacgcggcg ccaatcccca tcaatgagat cagaatcaac atcgcggcca ccgtgtaggg
     1621 gcgttttagt gcagcttcaa ccacggacat aaatgactct tggcactttt tcgcctagtc
     1681 cggtcgccgc tgcttgtggg tgaggattgc gagctcggta cgcgtattgg catggagttt
     1741 ctccatgatt cgactgacgt aattcttgac cgtaccctcg gcgagaaaca gcgtctgccc
     1801 aatctgccgg ttgctcctgc cctcggcgat gagctcgagt attttctcct ccttgtcgtt
     1861 cagagtctcg acgctggcaa cgccgtcgaa tgccggcggc cgttccgcgc tgcgcgcctc
     1921 cggttggtcg cgcgcaagac ggcggaattg gtccatcact ttgcgggcga tctgcggcgt
     1981 caggtgcgat tcgccgcgcc gcagggcgcg gatcgtcgcc aacaattcct cttccgccac
     2041 atctttgagc agataagcat gagcgccggc acgcacggcc tcgaacacgg tttcatcgtc
     2101 gtcgagtgtc gtcagcacga gaacctgggt cgtgggcagc gcgcgcgtga tttcccgggt
     2161 cgccgccacg ccacccttga ccggcatgtg caaatccatg agcaccacat ccggacgcag
     2221 cagtagcgtc agatctaccg cctcgacgcc gttactcgcc tggcccacca cttccatgtc
     2281 cggctcgatc gcaagcagca gcgcaacgcc cctgcgcacg atggcttgat cctcggcaat
     2341 gacgatgcga atcgccgccg gatttgccgc ctgccctaac gtcacacacg cctcgaattg
     2401 cagtccttgg acttcatgat gcggccactc ggcctgcgct tggaagtgac tttagtcacc
     2461 tgttaacgtg tgccgatgtc gaatgcgaaa ccgcaagagc cacgcagcct cgcctgggat
     2521 atcgatccga gacgcagcct ggctgcggct gccatgtggc tgatcgtcgg actggcgatc
     2581 acgttttcga ttgccgcggc actctgggtc ggcagcatcg cgcgcgccaa cgtcttggag
     2641 cagcatgtgc ggcggctgtc gctggaaacc gaacaactga gttcggactt gggccaagcg
     2701 ctggctgcgc ggctcggcgc aattcgcgcc gcccgcacct tggcgccgaa cgatctcgag
     2761 gatgtgttca atgaactggt atcggcctac cccgatctcg attggatcgc gattgcggac
     2821 accgcagggg tcgtcgtgag cactcggaat tccttgccgg cggggaccga gatcgcctcg
     2881 cgccgctggt ttgctgcggg attgcacgga ccttggctcg gcgtgatcga cgaacagccg
     2941 cgagcgtccg gcgttttcgg cgacatggcg gctccagtgc gggatcaggc cggccgcgtg
     3001 gtgggggtca tcgccgctca cttgagctgg cagcgggcgc caaaccaccc cgtgcgcttg
     3061 acggacgagg tcgatccgca ggtcgctacg caagcgtacg tgctcgaccg cgacggggcg
     3121 gtcctggttg ggcctgccga ttcgcgcggc tcacactggc agggaatcgg ggcgggcgcc
     3181 gcgtcggctg tgccgcaatt cgagcgcctg cccaacgggc gccggatact ggcctcacgc
     3241 gcgccgttga gtgccgcagg cgaagtagcg gcgttgggct ggcaggcgct gttgtgcgaa
     3301 cccatcgaac gggtgtatca gcgcgccgac gccttggcgc tgaagattct ctgggtatcg
     3361 ctctgcctcg gggccgcgac cgcatttctc gggatcatcg gcacgcgtca actcacccgg
     3421 cgtttgcaac ggctgacgca atcggtggca ttcctcgggc aaaacccaag cgcccggctc
     3481 gcggtgccgc agggactcga tgaagtggcg cagctgggcc gtgctttcgc cacgatccac
     3541 gatgatctgc agcgggagcg cagcgaattg cagaccttga gcggcgagct cgagcggcgc
     3601 gtcgccgtgc gcaccctcga ggtgcagcgc ctcgcagaag aatcgcggta tgcggccatc
     3661 gtccgcgagc gcttgaagat cgcccgcgat ttgcacgaca cactggcaca ttccatgatg
     3721 gccatgttga gcgaggtccg atttctgcgt cggctgctga ctcacgatcc cgctgctctg
     3781 ccggccgaac tcgcgcacgc cgaggaactc gcgcatgatg gactcaagga agcgcgtggc
     3841 gccatcacgc aaatgcgcgt cacggcggtt cgcgacatgg gcctcggtcc cgcgctggcg
     3901 aaggcgttcg agcgtttcat cgaccatacc ggtcttgccg gcgaattcac ggcgcaacct
     3961 gcggccgccc gcttcggcga cgaacgcgcc gagaccatct tcaacatggc gaacgaggcg
     4021 ttgcgcaaca tcgagcgcca cgcacgggca acccggatcg tggtttcgct caaatcagtc
     4081 gacgaaatgc acttggaatt gtgcatcgaa gacaatggcg tgggcttcga cgccagcgcc
     4141 gtgcgtccgg ggcactatgg catcgtggga cttcgcgaac aggcggaact catcggggcg
     4201 caattgcgaa tcgacagcag acccgaggag gggacgcggg tctgcgtgct atggagagtg
     4261 tcgccgatcg agttcatcgg cggcgccggc cgcgacgctc aaggcgccag caacagctcc
     4321 agtgtcctga agtagatgcg ctgcagccgg tcgatgtccg caacgcgcac gcactcgttc
     4381 accttgtgaa tggtctggtt gatcacgccc atttcgatga cttgcgcgcc cagcggagca
     4441 atgaaacggc cgtccgaggt gccgccgccg gtcgatagtt ttggggtgat ctgcagttgc
     4501 tcctgcacgg cgcgggtcgc ggcctgcgac agctcgccgg gagcggtgag gaatggctct
     4561 ccggatacaa accactcgag cgaatacttg acgcgatggc gccgcaacag ctcctcgacg
     4621 acgcgtttca gtccgtcgac gc
//
LOCUS       DASWZT010000015         2085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_14644, whole genome shotgun sequence.
ACCESSION   DASWZT010000015 DASWZT010000000
VERSION     DASWZT010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14644"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1366
                     /locus_tag="VGE92_00300"
     CDS             <1..1366
                     /locus_tag="VGE92_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEY1312280.1"
                     /translation="HRCGRIDVGINPRIIFSAFTAAFAGFLFGFDTVVISGAEQKIQA
                     LWSLGPGTHGVAMASALYGTVIGSLCGGWPSDRLGRRATLLWIGVLYLVSAVGSALAA
                     NVYQLIAARLIGGLGIGISTVAAPLYIAEIAPAAYRGRLAGMFQFNIVLGIVVAYLSN
                     ALLAGTGENAWRWMMGVAAFPSLLYSLLCLRVAESPRWLFIRRADRRAGMAALRSLYP
                     RAEDGDVQELAQQICAPAAPTSRPGAFWSKELRAPILLAVLVALFNQLSGINAILYFA
                     PRIFELTGLSEHAALLQSVGIGVTNLIFTFVGLWLIDRIGRRTLLFVGSFGYIFSLSL
                     VAWAFFTAHFSIVPACIFIFIAAHAVGQGAVIWVLISEIFPNQHRAEGQALGSFTHWS
                     AAALVTSVFPILVSVFAPGYVFAFFCAMMILQLIWVKTMVVETKGVPLEQVTQRLGSM
                     WYSG"
     gene            complement(1363..>2085)
                     /locus_tag="VGE92_00305"
     CDS             complement(1363..>2085)
                     /locus_tag="VGE92_00305"
                     /inference="COORDINATES: protein motif:HMM:NF005451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY1312281.1"
                     /translation="AEWETRINLAAAYRLIALFGWDDLVFTHVSARVQGPEHHFLINP
                     YGMMFEEITASSLVKVDLRGEKVSDSPYEINPAGFTIHSCIHAARADVNCVLHIHSLN
                     GVAVAAQVAGLLPISQQSLFVLASLGYHDYEGVALNPDEQPRLVRDLGDKTNLMLKNH
                     GLLTVGASMADAFLSMYVFESACAIQVRALSGGGELTHIAAPILQGIRAAAAQVTRGL
                     GGALVWPGLLRKLDRHSPGYRD"
BASE COUNT          342 a          615 c          662 g          466 t
ORIGIN      
        1 gcatcgctgc gggcggattg acgtggggat caacccgcgc atcattttct cggctttcac
       61 cgcggcgttt gccggctttt tgttcggctt cgataccgtg gtgatttcgg gtgcggagca
      121 gaagattcag gcgctatgga gtctcggtcc cggcacgcac ggcgtcgcga tggcctctgc
      181 gctgtacggc accgtgatcg gttcgctatg cggcggctgg cccagcgatc gactcggccg
      241 ccgtgctacg ttgttgtgga tcggcgtact gtatctcgtg tcggcggtcg gatccgcgct
      301 ggcagccaat gtttaccagc tgatcgcggc gcgcctgatc ggcggcctgg gcatcggcat
      361 ctcgacggtc gcggcgccgc tgtacattgc ggaaatcgcg cccgcggcgt atcgcgggcg
      421 cctggcgggc atgttccagt tcaacatcgt cctcggtatc gtcgtcgcgt atctgtccaa
      481 tgcgctgctt gccggcaccg gggagaatgc ctggcgctgg atgatgggcg tcgcggcgtt
      541 cccgtcgtta ctctattcgc tgctgtgcct gcgagttgcg gaaagcccgc gctggctgtt
      601 catccgcaga gccgatcgcc gtgcaggcat ggccgcactg cgctcgctct acccacgcgc
      661 ggaggacggc gacgtccaag aattggcgca gcaaatctgc gctccggccg cgccgacgtc
      721 gcgccccggc gccttctgga gcaaggagtt acgggcgccg atcttgctgg cggtgctggt
      781 cgcgctcttc aatcagctgt cgggcatcaa tgcgattttg tactttgcgc cgcgtatctt
      841 tgaactgacg ggcttgagcg aacacgccgc gctgctgcaa tcggtgggca ttggagtgac
      901 gaatctcatt tttacttttg tcggcctgtg gctgatcgat cgcatcggcc gccgcacgct
      961 gctgttcgtc gggtccttcg gctatatctt ctcgttgagc ctggtggcgt gggcgttctt
     1021 cacggcgcat ttcagcatcg tgcctgcctg tatcttcatc tttatcgcgg cacatgcggt
     1081 cgggcagggc gccgtcatct gggtgttgat ttccgaaata ttcccgaacc agcatcgggc
     1141 cgagggccag gcgctcggca gtttcacgca ttggagtgcg gcggcattgg tgaccagcgt
     1201 attccccatc ctggtttccg tgttcgcacc gggctacgtc ttcgcgtttt tttgcgcgat
     1261 gatgatcttg cagctgattt gggtgaagac gatggtcgtt gaaaccaagg gcgtgccgct
     1321 cgagcaggtg acgcaacggc tcggatcaat gtggtactcg ggctagtccc gatagcccgg
     1381 gctgtggcga tcgagtttgc gcagcagccc aggccacacg agtgctccgc ccaagccgcg
     1441 cgtcacttga gccgccgcgg cgcgaatgcc ttgtagaatc ggtgccgcga tgtgggtcag
     1501 ctcgccgccg ccgctcaggg cccgcacttg aattgcgcat gccgattcga atacatacat
     1561 ggacagaaag gcgtcggcca tcgacgcgcc gaccgtgagc agaccgtggt tcttcagcat
     1621 gagatttgtt ttgtcgccta agtcgcgcac caggcgcggc tgttcatcgg ggttgagcgc
     1681 gacgccctcg tagtcgtgat atcccaggga cgccaatacg aacagcgatt gttgtgagat
     1741 cggcaacaag cccgcaactt gcgcggcgac agcgacgccg ttcagcgaat ggatgtgcaa
     1801 gacgcaattg acatcggcgc gcgccgcatg gatgcagcta tgaatggtga atccggccgg
     1861 attgatttcg tacggtgaat cggagacttt ctcgccgcgt agatcgacct tgaccagcga
     1921 cgaggccgtg atttcctcaa acatcatccc atagggattg atcagaaaat gatgttccgg
     1981 cccctgaact cgagccgata catgggtgaa taccagatca tcccagccga acaacgcgat
     2041 caatcgatag gcggccgcca gattgatacg tgtttcccat tcggc
//
LOCUS       DASWZT010000016         4045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_33974, whole genome shotgun sequence.
ACCESSION   DASWZT010000016 DASWZT010000000
VERSION     DASWZT010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33974"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /gene="xseA"
                     /locus_tag="VGE92_00310"
     CDS             complement(<1..794)
                     /gene="xseA"
                     /locus_tag="VGE92_00310"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY1312282.1"
                     /translation="MQAADNQPLPRRPDRDIYSVSRLNREVRVLLERGFGSLWLEAEI
                     SNFSRPSSGHWYFSLKDATAQVRCAMFRQRNLLCTFGVRDGLKVLVRARIGLYEPRGE
                     YQLIVDHMEDAGLGALQRQFEELRTRLAAEGLFATQRKRPLPGLPRRVGIITSPSGAA
                     VHDILHVLARRFAAAAVLIYPVAVQGGNAAAEIIAALEAAGRRAECDVLILARGGGSL
                     EDLWAFNDERLARAIVASPIPVITGIGHEIDITIADFAADVRAPTPS"
     gene            876..2342
                     /gene="guaB"
                     /locus_tag="VGE92_00315"
     CDS             876..2342
                     /gene="guaB"
                     /locus_tag="VGE92_00315"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003295955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY1312283.1"
                     /translation="MRILEEALTFDDVLLVPAYSEVLPGEVSLASKLTREIQVNLPVI
                     SAAMDTVTEARLAITIAQEGGIGIIHKNMSIDAQARQVDRVKKFESGVISDPITVNPD
                     MTIRQVIELTRAKNISGVPVVLGTKVVGIVTHRDLRFETKLDAPVSSVMTPKDRLITV
                     RENAPKDEVLALLHKHRIEKVLVVAGNHELKGMITVKDFQKATEFPSACKDSVGSLRV
                     GAAVGTAPDTLKRVAALREAGVDVVVVDTSHGHSRGVLETVSKIKAQYSDIQVIGGNI
                     VTAEAALDLVKHGADGVKVGIGPGSICTTRVIAGVGVPQISAISRVAKALEGTGIPVI
                     GDGGIRYSGDVAKALAAGAHCVMIGGMFAGTEESPGDVELYQGGSYKAYRGMGSLGAM
                     AQAHGSSDRYFQDTTTELEKLVPEGIEGRVPYKGSLVAILHQLSGGLRAAMGYTGSSS
                     IEEMRTRPQFVRITSAGVRESHVHDVTITKEAPNYRIG"
     gene            2339..3916
                     /gene="guaA"
                     /locus_tag="VGE92_00320"
     CDS             2339..3916
                     /gene="guaA"
                     /locus_tag="VGE92_00320"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEY1312284.1"
                     /translation="MMADIHADRVLILDFGAQYTQLIARRVRELGVYSEIYACDVDAE
                     QIKRFAPKAIILSGGPESVYDVKGREAPAEIFSMGVPVLGICYGMQTMAAQLGGQVES
                     SRHREFGAAQVRPASGARLFDGLEDNRDAQGRRVLDVWMSHGDHVVAVPPGFKVIGSS
                     DNAPFAAMADESRRFYGLQFHPEVTHSLQGGEILRRFVREIAACSANWATANIIEDSV
                     SRIRARVGADKVLLGLSGGVDSSVLAALLHRAIGAQLTCVFVDHGLLRLGEGDQVMAT
                     FAEHMGVRVVRVDAEQRFLAALAGEADPEQKRKIIGRLFVEIFDEEAEKLKDVKWLAQ
                     GTIYPDVIESAGSKTGKAHVIKSHHNVGGLPQHMKLQLLEPLRELFKDEVRKLGLELG
                     LPREMVYRHPFPGPGLGVRILGEIRKPYADLLRRADAIFIEELRRHELYDKVSQAFAV
                     FLPVRSVGVMGDGRRYDHVVALRAVETVDFMTARWARLPYEFLDRVVHRIINEVPGIS
                     RVVYDVSGKPPATIEWE"
BASE COUNT          793 a         1196 c         1332 g          724 t
ORIGIN      
        1 gacggggtgg gcgcgcgcac gtcggcggca aaatcggcaa tggtaatatc gatctcgtga
       61 ccgatccccg tgattaccgg aatcggcgag gccacgatgg cgcgcgccaa gcgttcgtca
      121 ttgaatgccc acaaatcctc caaggaaccg ccgcctcggg ccaagatgag cacatcgcac
      181 tcggcacgtc gcccggcggc ctcgagcgct gcaatgatct cggcagcggc gttccccccc
      241 tgcacggcta ccgggtagat caggaccgcg gctgcggcaa agcgcctcgc aagcacgtgc
      301 aggatgtcgt gaaccgcggc gccgctcggc gaggtgatga tgccgacccg gcgcggtagg
      361 cccggcaggg ggcgcttgcg ctgcgtcgcg aacaggccct cggcggcgag tctggtgcgc
      421 agctcctcga actgccgctg cagggccccg aggcccgcgt cctccatatg atcaacgatc
      481 agctggtatt cgccgcgcgg ctcatagagc ccaatccgcg cgcgcaccag cactttcagt
      541 ccgtcccgca ccccgaatgt gcacagcaaa tttcgctgcc gaaacatggc gcaacgaacc
      601 tgggcggtgg cgtctttcag cgagaaatac cagtgtcctg aactgggacg cgaaaaattc
      661 gagatctccg cctccagcca gagactgcca aatccacgct cgagcagcac tctgacctcg
      721 cgattgaggc gcgaaacgct gtaaatgtcg cgatccgggc gccgcggcag gggctggttg
      781 tcggctgctt gcatggacaa atgatagcgg tttaccgcgc agcagtcgca aggtacaatg
      841 cgccgcctct tagatgaacc cgtgaacgct cccctatgcg tattcttgaa gaggcactca
      901 cattcgatga cgtcctgctg gtgccggctt actctgaagt gctgcccggc gaagtgtcgc
      961 tcgccagcaa gctgactcgc gaaatccaag tcaatctgcc ggtgatttcc gcggcgatgg
     1021 acaccgttac ggaggcgcgc ctggcgatta ccatcgccca agagggcggt atcggcatca
     1081 tccacaagaa catgtcgatc gatgctcagg cgcggcaagt cgaccgcgtc aagaagttcg
     1141 agagcggcgt gatcagcgac ccgatcaccg tcaatcccga catgacgatt cggcaagtca
     1201 tcgaactcac tcgcgcgaag aacatttccg gggtgccggt ggtgctcggt accaaagtgg
     1261 tcggcatcgt cacgcaccgc gatctgcgct tcgagaccaa gctcgatgcg ccggtatcct
     1321 cggtgatgac gccgaaagat cgcctgatta ccgtgcgcga gaatgcgccg aaagacgaag
     1381 tgctcgcgct gctgcacaaa caccgcatcg agaaggtgtt ggtggtggcc ggcaatcacg
     1441 aactcaaggg catgatcacg gtcaaggatt ttcagaaggc caccgaattt ccaagcgcct
     1501 gcaaggacag cgtcggaagc ctgcgcgtcg gcgccgccgt cggcaccgca cccgacacct
     1561 tgaagagagt cgcggcgctg cgcgaggcgg gagtcgacgt cgtggtggtc gatacctcgc
     1621 atgggcattc gcgcggagtg ctggaaacgg tgtcgaaaat caaggcgcag tattccgaca
     1681 tacaggtgat cggcggcaat atcgtgaccg ccgaggcggc gctcgatctc gtcaagcacg
     1741 gcgcggacgg ggtaaaggtg ggcatcgggc cgggatcgat ctgcacgacg cgcgtgattg
     1801 ccggcgtcgg cgtcccgcaa atcagcgcaa tttcgcgagt tgcgaaggca ttggagggta
     1861 ccgggatccc ggtcatcggt gacggcggaa tccgctattc cggcgatgtc gccaaggcgc
     1921 tcgcggcggg cgcgcactgc gtcatgatcg gcggaatgtt cgcgggcacc gaggaatctc
     1981 cgggtgatgt ggagctctac cagggcggct cctataaggc atatcgcggc atgggatcat
     2041 tgggcgcgat ggctcaggcg cacggttcca gcgatcgtta tttccaagac accacgacgg
     2101 agctggaaaa gctcgtgccc gaaggaatcg agggacgcgt gccgtataaa ggcagcctgg
     2161 tcgcgattct gcatcagctg tcgggcggcc tgcgcgccgc catgggatac acgggcagca
     2221 gcagcatcga agagatgcgc acgcgtccgc aatttgtgcg aataaccagc gccggggtgc
     2281 gcgagagtca cgtgcacgat gtgaccatca ccaaagaagc gcccaactac cggatcggtt
     2341 gatggccgat attcacgcgg accgggtcct cattctcgac ttcggcgcgc agtacaccca
     2401 gctgatcgcg cgccgcgtgc gtgaactcgg cgtgtattct gaaatctatg cctgcgatgt
     2461 cgatgcggag caaatcaagc gcttcgcgcc gaaggccatc atcttgtcgg gcggccccga
     2521 gtcggtgtac gacgtcaaag gccgcgaagc gccggccgag atattttcga tgggagtgcc
     2581 cgtgctcggc atctgttacg gcatgcagac catggccgcg cagttgggcg gtcaggtcga
     2641 gtcgtcccgg catcgcgagt tcggcgccgc gcaggtgcgg ccggcgagcg gcgcacggct
     2701 cttcgatggc ctcgaagaca accgcgatgc tcaaggacgg cgagttctcg atgtgtggat
     2761 gagccacggc gaccacgtcg tcgccgtgcc gccgggattc aaagtgatcg gctcgagcga
     2821 taacgcgccg ttcgcggcga tggcggatga gtcgcggcgc ttctacggac tgcagtttca
     2881 ccccgaggtg acccacagcc tgcaaggcgg cgagatacta cggcgcttcg ttcgcgagat
     2941 cgccgcttgt tcggcgaatt gggcaacggc caacatcatc gaggacagcg tttcgcggat
     3001 tcgcgcacgg gtcggcgcag acaaggtttt gctgggtctg tcgggcggcg tcgactcgtc
     3061 ggtgttggca gcgttgctac atcgcgccat cggcgcacag ctgacttgcg tgttcgtgga
     3121 ccatggtctg ctgcggctcg gcgaaggcga ccaagtaatg gcgacgtttg ccgagcacat
     3181 gggcgtgcgc gtggtacgcg tcgatgcgga gcaacgtttt ctggcggcac tcgccggcga
     3241 ggcggatccg gaacagaagc gcaagatcat cgggcgcttg ttcgtggaga tattcgatga
     3301 ggaagcggag aagctcaagg acgtgaagtg gctcgctcag ggaacgatct atcccgacgt
     3361 gatcgagtcg gcgggcagca agaccggcaa ggcgcacgtg atcaagtcgc accacaacgt
     3421 gggtggattg ccgcagcaca tgaaattgca gctgttggag ccgctgcgcg aattgttcaa
     3481 ggatgaggtc cgcaagctcg gactggaact cggattgccc cgggaaatgg tctaccggca
     3541 tcccttcccg ggccccggtc tcggcgtgcg gattctagga gagatccgca agccatatgc
     3601 cgatctgctg cgccgtgcgg atgcgatttt catcgaggag ctgcgccgcc atgaactgta
     3661 cgacaaagtg agtcaggcgt ttgccgtttt cttgccggtg cgttcggtcg gtgtcatggg
     3721 cgatgggcga cgctacgatc atgtcgttgc gctgcgggcc gtggagaccg tcgatttcat
     3781 gaccgcgcgt tgggcacggc taccctacga gttcctggat cgagtcgtac atcgaatcat
     3841 caacgaagtg ccgggaattt cgcgcgtggt gtacgacgtt tccggaaagc cgccggcgac
     3901 gatcgagtgg gagtgaccag accagggcgc ttccctagtg tcgcacgatc ggcagagagg
     3961 gaaccctgag tttcggggca aaactggcaa taactcacct ctatccatcg cgcgcagatg
     4021 gcaccggagt cgcttctgct acgta
//
LOCUS       DASWZT010000017         6547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_4945, whole genome shotgun sequence.
ACCESSION   DASWZT010000017 DASWZT010000000
VERSION     DASWZT010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6547
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4945"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VGE92_00325"
     CDS             <1..491
                     /locus_tag="VGE92_00325"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY1312285.1"
                     /translation="IMVGGDEAVYERALPILQAMGKNVAYLGSSGAGQAAKATNQIMC
                     AGIIQAVGEAMAFAHAEGLPLDRLIELLGKGAGSSWYFVNRAPFMAHNNYPAGFRVRL
                     HAKDLRICHEMAARHGAQLPVVESTLAQYAELIAQGFGDEDISAIYRLKSALFPRDPK
                     PA"
     gene            488..1426
                     /gene="hemC"
                     /locus_tag="VGE92_00330"
     CDS             488..1426
                     /gene="hemC"
                     /locus_tag="VGE92_00330"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005108084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY1312286.1"
                     /translation="MNAPPAAPLRIATRKSQLALWQAEHVAQLLRRAHAGVAVELVPI
                     VTQGDRIQDRSLAAIGGKGLFIKELEIALEERRADIAVHSMKDLPGELAPGLSIAAVL
                     ERADARDALLTSASASLDGLPRGARVGTSSLRRQAQLLAARPDLKIEALRGNVDTRLR
                     RLDAGELDAIVLACAGLIRLGLEYRITARLEPRVSLPAVAQGVIGIECRSGDLRSLHL
                     LSVLNHRDTRTAVDAERAFAQRLGGSCQSPIAAHAELEGKRLTLEGLVAEPDGSRLLR
                     DTMTGSADEPTALGHALAERILAAGADRLLERLRAG"
     gene            1429..2208
                     /locus_tag="VGE92_00335"
     CDS             1429..2208
                     /locus_tag="VGE92_00335"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY1312287.1"
                     /translation="MSALHGVGVLVTRPEHQAMPLCRLLEMQGATTLRLPAVEIKACG
                     NRRDIAARLGALEEFDLIVFTSSNAVRFGAPLLDQKRDLTLAAIGPATARALDQGGYR
                     VAVQPQAGFDSESLLKHPRLEHAAGRRILIIKGSGGRPLLEQELMQRGAQVSTADVYH
                     RVQATPDHAALAVLLNRFTDGAVQVITATSLEIGSSLLGLAPPALREQFEQAHWLVPG
                     ERIAAGLRERGLSAPLLQAESAEDHDLVAALIRWRSSVSGA"
     gene            complement(2154..3191)
                     /locus_tag="VGE92_00340"
     CDS             complement(2154..3191)
                     /locus_tag="VGE92_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY1312288.1"
                     /translation="MRVILTQSDRSFNLAADQSLLDAALGASLNLPHSCKSGNCGACR
                     ARLLQGAVYYPNGPPLGLSDAEIADGYVLMCQARARGDVSLEILEMATPEQILIKRLP
                     ARIERARPLSHDVMGLYLKLPAAEDFHFEAGQYIDVMLPGGRRRSFSIASPPHDARPL
                     ELHVRRVAGGEFSAPLFDANMDSTLLTIEGPLGQFTYRAAAAPMLLIGGGTGLAPLLS
                     ILRHVIETGLERDMQLYWGVRSERDLYAHALLEALARRTARLHYVPVLSEPSPEWSGA
                     RGLVHEAALCDIHDLDHYDVYAAGPPAMISVVRSEFGRRGVAPSRLFFDSFDYAPDTL
                     ERQRMSAATKS"
     gene            complement(3195..3839)
                     /locus_tag="VGE92_00345"
     CDS             complement(3195..3839)
                     /locus_tag="VGE92_00345"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY1312289.1"
                     /translation="MRHVMVLNAKGGCGKSTLATNLAVFFARDGHQVCIADYDPQRSS
                     LDWLAQRPADLPAISGVAAYEEGLRSVARNTEILVIDAPARVHGTELNELVRRAETII
                     VPVLPSSIDMKACGHFMAELRELGKVSRKQARLAVVANRVREYTLIYEELDEYLKKLK
                     IPYLGSLREAQNYVRAYARGMGVLELPEYLAWPDWKQWEPIGEWLDSKRSQPAG"
     gene            3939..4766
                     /gene="dapF"
                     /locus_tag="VGE92_00350"
     CDS             3939..4766
                     /gene="dapF"
                     /locus_tag="VGE92_00350"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEY1312290.1"
                     /translation="MRIEFVKMQGLGNDFFVFDAPAAGFHPSKDRLRALADRHTGVGF
                     DQALLLGTPRDAHSRVFYRIFNSDGTEVEQCGNGARCIAALLYARAPHLGRDLTMDSS
                     GGIVSAHVRDDGLVSVDMGVPNFDPRALPMHADAEADFYTLNVAGTDFEIGAVSMGNP
                     HAVLEVADVKTAPVAQIGPSIERHPRFPKRANVGFMEILTRNHLRLRVYERGAGETLA
                     CGTGACAAVAVGRRRGRLDAEVRVDLPGGSATVSSAQDASLWLTGPATTVFTGSIDI"
     gene            4789..5496
                     /locus_tag="VGE92_00355"
     CDS             4789..5496
                     /locus_tag="VGE92_00355"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HEY1312291.1"
                     /translation="MTISQARGIKQESLNDAGVADYLQSYPDFFERNSSLLAKLRLPH
                     LRDVGATVSLVERQVEVLRERNQSLERKLKELVDVARANDALADRIHRLSQRLIRAHT
                     LLETIAAIETSLREDFEAMHSVLVLFIEQASSLEAAAGRFLRYGHPADADIKTFESLL
                     QSGKPRCGQVRDGQRDYLFGKESIEIGSVALTPLGPKGELGILAIGASDAERFHPAMS
                     TEFLSRIGELVTYALMR"
     gene            5510..6418
                     /gene="xerC"
                     /locus_tag="VGE92_00360"
     CDS             5510..6418
                     /gene="xerC"
                     /locus_tag="VGE92_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008040629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEY1312292.1"
                     /translation="MESGAREWIDRFLSHLAHERRLSAHTLAAYRRDLQGLAEFGARR
                     QLDWDGFDNSHLRAFAAGLHGGGLSPRSVQRNLSAARTFYEFLIREGKCARNPARDVR
                     APKAKKRLPETLDADLMGRLLAFRADDSLSARDKAIMELFYSSGLRLAELVGLSRGDI
                     DLKDRTVRVLGKGSKTRIVPIGRQAIDALLKWLKERAALAEVGVEALFVGRSGRPLSV
                     RAVQLRVGAWGRRQGIGVHVHPHMFRHSFATHLLESGGDLRGVQELLGHANIGTTQIY
                     THLDFQHLASVYEAAHPRARRRPGRA"
BASE COUNT         1182 a         2056 c         2134 g         1175 t
ORIGIN      
        1 ccatcatggt gggcggcgat gaagcggtgt atgagcgcgc gctgcccata ttgcaagcga
       61 tgggcaagaa tgtcgcttat ctcggctcga gcggtgccgg acaggcggcg aaggccacca
      121 atcaaatcat gtgcgcgggc atcatccaag cggtcggcga ggcgatggcc tttgcgcacg
      181 ccgaaggcct gccgctcgat cgattgatcg agcttctggg caagggcgcg ggctcgagtt
      241 ggtacttcgt gaatcgcgcg cccttcatgg cgcacaacaa ttatcctgcc ggttttcgcg
      301 tgcgcttgca tgccaaggac ttgcggattt gccacgagat ggcggcgcgc cacggcgcgc
      361 aactgcccgt ggtcgagtcc actctggcgc aatacgcaga gctcattgcc cagggtttcg
      421 gcgacgagga tatttcggcc atctacaggc tcaagtccgc actgttcccc agagatccca
      481 agccggcatg aacgcgccgc cggctgcgcc gctgcgcatc gccacacgca agagtcagct
      541 cgctctgtgg caggccgagc atgtcgcaca gttgctgcgc cgcgcgcatg cgggtgttgc
      601 cgttgaactc gtgcccatcg tgacccaggg cgatcgtatt caagaccgca gcttggcggc
      661 gatcggcggc aagggtttgt tcatcaagga actcgaaatc gctcttgagg agcggcgcgc
      721 ggatattgcc gtgcattcca tgaaggacct gccgggtgaa ctcgcgccgg ggctcagcat
      781 cgcagcggta ctcgagcgcg ccgatgcccg ggatgcattg ctgacgtcgg cgtcagcctc
      841 actcgatgga ttgccgcgcg gtgcgcgtgt cggtacttcg agcctgcgcc gccaagcgca
      901 attattggcg gctcggcccg acttgaagat cgaagcgctg cgtgggaatg tcgacacgcg
      961 gctgcgacgg ctcgatgccg gcgaactcga cgccatagtg ctcgcgtgcg ccggcctgat
     1021 tcggctcggc ttggagtacc gaattaccgc acgcctcgag ccgcgcgtgt cgctgccggc
     1081 ggtcgcgcag ggtgtgatcg gcatcgaatg ccgcagcggg gacttgcgct ccctgcatct
     1141 gctgtccgtg ctcaatcatc gcgacacgcg aactgccgtg gacgcggagc gcgccttcgc
     1201 gcagcgcctg ggcggcagct gtcaatcgcc gattgcggcg catgcagagc tcgagggaaa
     1261 gcgactgacg ctcgagggct tggtagcgga accggatggc tcgagactgc tgcgcgacac
     1321 gatgacgggc agcgccgatg agccgaccgc gctcggccac gcgttggcag agcgcatcct
     1381 cgccgccggt gcggacagac tgctcgagcg gttgcgcgcc ggctgaacat gtccgcattg
     1441 cacggcgtcg gcgtcctcgt cacccgcccc gagcaccagg cgatgccgct gtgccggctg
     1501 ctcgagatgc aaggcgcgac cacgctgcgc ctgccggccg ttgaaatcaa agcatgcggc
     1561 aatcggcgcg acatcgcggc aagactcggc gctctcgagg aattcgacct cattgtattt
     1621 accagcagca acgccgtccg attcggcgcg ccgctgctcg atcagaagcg cgatttgacg
     1681 cttgccgcga taggtcccgc gacggcgcgc gcgctcgatc aaggcggtta ccgcgtggcg
     1741 gtgcagccgc aggcaggatt cgattcggag agtttgctca agcatccgcg gctcgagcat
     1801 gcggcgggcc gacgcatcct catcatcaag ggcagcggcg gccggccgtt gctcgagcag
     1861 gaactcatgc agcgcggcgc gcaggtgtcc accgccgatg tctaccaccg cgtgcaggcg
     1921 acgcctgacc acgccgcgct tgcagttctg ctgaatcgct ttacggacgg agccgtgcaa
     1981 gtgatcaccg cgaccagctt ggagatcggc agcagcctcc tcgggctggc acctcccgcg
     2041 ctgcgcgaac aattcgaaca ggcgcactgg ttggtgcccg gcgagcgcat tgcagccggt
     2101 ctgcgggagc gaggcttgtc ggcaccgcta ttacaggctg agtcggccga agatcacgat
     2161 ttggtcgcgg cgctcatccg ctggcgctcg agcgtgtccg gggcgtaatc gaaagaatcg
     2221 aaaaacaagc ggctcggcgc caccccgcgc cggccgaatt cgctgcgcac cacgctgatc
     2281 atcgccggcg gccccgcggc gtatacatcg taatggtcga gatcgtggat gtcgcagagc
     2341 gccgcctcat ggaccaagcc gcgggcgcca ctccattcgg gcgagggttc ggagagcacc
     2401 ggcacgtaat gaagccgcgc cgtgcgccgc gcgagcgctt cgagcaaggc atgcgcgtaa
     2461 aggtcccgct cgctgcgcac tccccaataa agctgcatgt cgcgctcgag cccggtctcg
     2521 atcacgtgcc gcagaatgct gagcagcggc gcaagtccag tgccgccgcc gatcagcaac
     2581 atcggcgctg cagccgcgcg gtacgtgaat tgaccgagag gtccttcgat ggtcaggagc
     2641 gtgctgtcca tgttggcatc gaacagcggc gccgagaatt ctccgccggc aacccgacgc
     2701 acatgcagct cgagcggtcg ggcgtcgtgc ggcggcgagg caatcgaaaa actgcggcgc
     2761 cggccgccgg gcagcatgac atcgatgtat tggcctgcct cgaagtggaa atcctcggcg
     2821 gcgggaagct tgagatacag gcccatgaca tcgtgcgaca acggccgcgc gcgctcgatt
     2881 cgcgcgggca gccgcttgat caggatttgc tcgggagtcg ccatctcgag aatctcgagc
     2941 gacacgtcgc cgcgcgcccg ggcctgacac atgagcacgt agccgtcggc gatctcggca
     3001 tcggacaacc ccaacggcgg accgttggga tagtagacag ctccctgcag caggcgggcg
     3061 cggcacgcgc cgcaattgcc gctcttgcag ctgtgcggca aattcagcga agcgccgagc
     3121 gccgcgtcga gcagcgactg atccgctgca aggttgaagc tgcgatcgga ttgagtcagg
     3181 atgacgcgca gagcttagcc tgctggctgg ctacgcttgc tgtccaacca ttcgccgatg
     3241 ggctcccact gcttccagtc cggccaagcc agatattccg gcaattcgag tacgcccatg
     3301 ccgcgcgcgt aggcgcgcac gtaattctgc gcctcacgca agctgcccaa gtaaggtatt
     3361 ttgagcttct tcagatactc gtcgagttct tcgtaaatca aggtgtattc gcgcacgcgg
     3421 ttggcgacga ccgccaaccg ggcctgcttg cgcgacacct tgccaagctc gcgcaattcc
     3481 gccatgaagt gcccgcaggc cttcatgtcg atgctggaag gcagcaccgg aacgatgatg
     3541 gtctcagccc ggcgcaccag ctcgttgagc tcggtgccgt ggacacgggc cggcgcatcg
     3601 atcaccagga tttccgtgtt gcgtgcgacg ctgcgcagac cctcttcgta cgcggcaaca
     3661 ccgctgatcg ccggcaaatc ggccgggcgt tgtgccagcc agtcgaggct ggagcgttgc
     3721 ggatcgtagt ccgcgatgca cacctgatgt ccgtcgcggg caaaaaaaac ggcgagatta
     3781 gtcgccagag tgctcttgcc gcagccgcct ttcgcattca ataccattac gtgacgcata
     3841 atcccccgac gttgttgttt tcagtaaggt agaactcgga cgtagattgc gcaacccccg
     3901 ctcgcgacaa atcgacgcat cggctatgct ctcgtagcat gcgcatcgag tttgtgaaga
     3961 tgcagggact cggcaacgat ttctttgtat tcgacgcccc ggccgccggt tttcatccaa
     4021 gcaaagatcg gttgcgcgcg ctcgcagatc ggcataccgg agtcgggttc gatcaagcgt
     4081 tgctgctcgg gactccccgc gatgcgcaca gccgcgtgtt ctatcgcatc ttcaattccg
     4141 acggcaccga agtcgaacag tgcggcaacg gcgcgcgctg tattgcggct ctgttgtacg
     4201 cgcgggcacc gcacctcgga cgcgacctga cgatggacag cagcggcgga atagtcagcg
     4261 cgcacgtcag agacgatggc ctggtgtcag tggacatggg tgtacccaat ttcgatccgc
     4321 gcgcgctgcc gatgcacgcg gacgccgaag cggatttcta cacattgaac gtggcgggca
     4381 cggatttcga aatcggcgcg gtctcgatgg gaaacccgca tgcggtgctc gaggtggccg
     4441 acgtgaaaac cgcgcccgtt gcgcaaattg ggcctagtat cgagcgacat ccacgatttc
     4501 cgaagcgcgc caacgtcggg ttcatggaaa ttttgacgcg caatcacctg cgattgcgcg
     4561 tatatgaacg cggagcgggc gagacattgg cgtgcggcac cggggcgtgc gcggccgtcg
     4621 ccgtcggcag gcggcgcggc agattggacg ccgaggtgcg cgtcgacctg ccgggcggta
     4681 gcgcaacggt ttcttcggcg caagatgcga gtttgtggct cacagggccg gcgactacgg
     4741 tgtttaccgg atcgatcgac atttaacgtg cacaacggga gctgaataat gaccattagc
     4801 caggcgcgcg ggatcaagca agagagcctc aacgatgccg gtgtggcgga ttacctgcaa
     4861 tcctatccag atttcttcga acgcaattcc tcgctgctgg caaagctgcg cctgccgcat
     4921 ctgcgcgatg tgggtgccac cgtcagtttg gtggagcgcc aggtcgaagt gctgcgcgag
     4981 cgcaaccagt cactcgaacg caaactcaag gaactcgtcg acgtagcccg cgccaacgac
     5041 gccttggccg atcgtatcca tcgtttgagc caacgtctga ttcgcgcgca cacattgctc
     5101 gagacgatcg ccgccatcga aacctcgctg cgcgaggatt tcgaagccat gcattcggtg
     5161 ctggtgttgt tcatcgagca ggcatcgagc ttggaagcgg cagccggccg attcttgcgc
     5221 tatggccatc cggcggatgc cgacatcaag actttcgaat cgctgctgca atccggcaag
     5281 ccgcgctgcg gacaagttcg cgatgggcag cgcgattatc ttttcggcaa ggaatcgatc
     5341 gagatcggct cggtggccct cactccgctc gggcccaagg gcgaattggg gatattggcc
     5401 atcggcgcca gcgatgcgga acgtttccac ccggccatga gcacggagtt cctgtcgcga
     5461 atcggtgagc tcgtgaccta cgcgctcatg cgctaggacg ccgatcgccg tggagtcggg
     5521 ggcgcgcgag tggatcgatc gttttctatc gcaccttgcg cacgagcgtc gtttgagcgc
     5581 acacaccctt gccgcctatc gccgcgatct gcaaggcttg gccgagttcg gcgcgcggcg
     5641 ccaactcgac tgggacgggt tcgacaactc ccacctgcgg gcattcgcgg ccgggctgca
     5701 tggcggcgga ttgagcccgc gcagcgtgca gcgcaatttg tcggcggcgc gaactttcta
     5761 cgaattcctg atacgggaag ggaagtgtgc ccgcaatccc gcgcgggatg tgcgcgctcc
     5821 caaggctaag aagcggctgc cagagacttt ggacgccgat ctgatgggac ggctgctggc
     5881 atttcgcgcc gacgattccc tatcggcacg cgacaaagcc atcatggagt tgttttactc
     5941 ttcgggattg cgattggcgg agctcgtcgg cttgagccgc ggcgacatcg acctcaagga
     6001 tcgcacggtg cgcgtgctcg gcaagggcag caagacgcgc atcgtaccaa tcggccgaca
     6061 ggccatcgac gcgttgctga agtggctcaa agaacgcgcg gcattggccg aggtcggcgt
     6121 cgaggcgctt tttgtagggc gcagcggccg cccgctgtcg gtacgcgccg tccagctgcg
     6181 agtcggcgcc tggggccgtc gccagggcat cggtgtgcac gtccacccgc atatgttccg
     6241 ccattccttt gccacccatt tgctcgagtc gggcggcgat ttgcggggcg tgcaggagct
     6301 gctcggccac gccaatatcg gcaccacgca gatctacacc cacctcgatt tccagcatct
     6361 ggccagcgtc tatgaggcgg cgcacccacg ggcgcggcgc cgcccaggcc gtgcttgaaa
     6421 gggccggcat cggccccacg catgaggttg ctatgaacca tgatccgaag cgaatctacg
     6481 gcaccaccat tctcgcggta cgccgccagg ggaaggttgc ggtcggcggc gatggccagg
     6541 ttacgct
//
LOCUS       DASWZT010000018         6448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_67921, whole genome shotgun sequence.
ACCESSION   DASWZT010000018 DASWZT010000000
VERSION     DASWZT010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6448
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67921"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VGE92_00365"
     CDS             <1..581
                     /locus_tag="VGE92_00365"
                     /inference="COORDINATES: protein motif:HMM:NF044952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312293.1"
                     /translation="AERHVIGFRQGVPVSLDGSAGSAVAIIEKLEALAGPFGIGRGIH
                     LGDTVIGTKGRVAFEAPAADVLLTAHRELEKLVLTGKQARIKESVAQPYGDLVHEGQH
                     LDPVCRDIEALLVSSQARVSGEVTVLFRPGSVFVEGVSSPYSLMAASKGVYGESAGEW
                     TPADALGYSKMLALTGVFYKRAGSHAARAEQS"
     gene            578..1666
                     /locus_tag="VGE92_00370"
     CDS             578..1666
                     /locus_tag="VGE92_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312294.1"
                     /translation="MSSMRTVVVDKIASVTQACGLSHEIRVAADIPCDEGVVVVVEIL
                     NNKSTYNTLELTSGRMAKIARGDVVVGALGHRKALFGYSGHIPLALKPGDVIQMLNIG
                     GVLGICDSATPDKGKPFDCKVVGTALTFPYLGERIGVPARAGHKALDYEAALDVRGVP
                     VVALAGTCMEAGKTAAASAIIARMRHRGLIVDAFKATGVSLRRDILAFEDSGARRTMI
                     FTDLGVVTTTAKCGPALTRTMLTEMSAGQPDVIVFELGDGLLGTYGVESILRQPDIKA
                     ALTAVVLSANDPVAAWGGVKLLRERFGIEPAVVTGPATDNQVGVEIIREQMNVEAFNA
                     ISSPADLGDHIIGRLGLSHVRESKVAAE"
     gene            1663..2724
                     /gene="argC"
                     /locus_tag="VGE92_00375"
     CDS             1663..2724
                     /gene="argC"
                     /locus_tag="VGE92_00375"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEY1312295.1"
                     /translation="MSTATPAIVLGGTGYVAGELLRLIAGHPQLEVAAILSDSQPGAS
                     VAAAFPHLCSSYPELKFADIDAIETLIRRTSQSALFSAAPHGVAAAIIDRLLNAAGAA
                     GTRVRCVDISADFRYSSAAAYEAVYKHAHGAPARIRDFTCAVPEHLQKSPTPHIAHPG
                     CFATATLLATVPLLALGLTDNRLFVTGITGSTGSGRKPIEGTHHPHRHSDLYSYGALS
                     HRHVPEIIACAKLATGVEPELNFVAHSGPFARGIHVTAQASLKKPIGTAQAVEAFKAY
                     YKHSPFVRVSEAAPRVKDVVSSNYANLSTVTNGDSIAVMSAVDNLNKGAAGGAIQWMN
                     RLLGLPESTGLTAGAPGWT"
     gene            2730..3881
                     /locus_tag="VGE92_00380"
     CDS             2730..3881
                     /locus_tag="VGE92_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY1312296.1"
                     /translation="MTAPTVISHLAQVFAQYPIEVAHGEGVWLYARDGRKVLDFYGGH
                     AVAGLGYGHPRWVAALERQARQMAFQTNALPMLIREHAAARLTKFAGLGLDTVFWINS
                     GAEANENALKLAFKLSGRSKIVALEQGWHGRTAAAGAVTWGALEKWYGFPRTPFDVSF
                     APRDNPDALGQFIDRHTAAVIVEPVQGVGGAYEVGQPMLRALRQRCDETGALLIFDEV
                     QCGMGRTGAPFAANFYGVVPDMLTSAKALGNGFPVSALLLSQRVAGRIKYDDMGTTFG
                     GGPMACAAAEAVIDTIESESLLANVRQVSAYIRSSCIVGPVTGVQGAGFLLGLKTSRP
                     AKEVHAELLAKNILAGTSADPHILRLLPAYILNEGHVDQLSDALSRISA"
     gene            3878..4873
                     /locus_tag="VGE92_00385"
     CDS             3878..4873
                     /locus_tag="VGE92_00385"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF003384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HEY1312297.1"
                     /translation="MKRFLKLADFSREQVLDLLALAQALQDQPDSRALAGKILGLVFM
                     NPSLRTLASFQAAMARLGGSCFVITPGQGTWQLETRPNATMNGAAAEHIREGIPVLAS
                     YCDALGVRAFAEGKNLESDLQETLFGMIDALCDKPLINLESAMNHPCQALADWRTLDE
                     LKVPRRAKFVLSWVNHPRALPLAVPAATLHMAAMRGMEVVVLRPHGFALPAPIMDGAC
                     AAAAAAGGAVSETSDRAAALAGAHVLYAKEWGSTQHYGDSEADSRLRAELADWCVREQ
                     WFAAAAPDAKLMHCLPVRRNVAVADEVLDGPRAVVKREAFNRLAVQMAVLHRMLT"
     gene            4905..5837
                     /gene="argB"
                     /locus_tag="VGE92_00390"
     CDS             4905..5837
                     /gene="argB"
                     /locus_tag="VGE92_00390"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEY1312298.1"
                     /translation="MSRPDPSVAVRALKSAAPYIRMYKNKVFIIKAGGAVFSDEISTR
                     ALIEQVAILHQVGIKTVLVHGGGPQLDSMQASLGIDTRMVHGRRVTDQKSIDVTAMVL
                     NGLINTRILAICRELDIEAIGLSGVDAGLIRAHKRPPMRVPDSSGEFVDYGFVGNIDS
                     VNTGVIEKLLENGLMPVVSPLSADAHGTLLNINADTVAAAIGGALSAEKLVLCTGAQG
                     ILERLDDASSVVSYTDIKGLKRLRDQGALKDGMLPKAAAIENAIRGGVRRVHVIGYKS
                     TDSLLAEIFTNEGTGTLVVADLTALSPAEQSSAR"
     gene            5834..>6448
                     /locus_tag="VGE92_00395"
     CDS             5834..>6448
                     /locus_tag="VGE92_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEY1312299.1"
                     /translation="MTRLWDKGIALDSQVLAFTAGDDHLLDNRLVSYDIRASIAHAEM
                     LAVQGLLSAPDLESIRDALTAIGSEHAQGQWRIVLEQEDCQTAIENLLTARVGAAGGR
                     LHAGRSRNDQVLAALRLYMRDAATELHQGAAAVASALDALAARHGAVVLPGYTHMQQA
                     MPSSVALWAQGFAAEIRDDAEGLALTQRRIGKNPLGSAAGYGTPN"
BASE COUNT         1147 a         2011 c         2121 g         1169 t
ORIGIN      
        1 gcgccgagcg ccacgtgatc ggttttcgac aaggagttcc cgtgagtttg gacggcagcg
       61 ccggctcggc ggtcgcgatc atcgagaaac tagaagcgct ggcgggtccg ttcggcatcg
      121 gccgcggcat ccacctcggc gacaccgtga tcggcaccaa ggggcgggtc gcgttcgaag
      181 cacccgccgc cgacgtgctg ctgactgcgc atcgggaatt ggagaagctg gtattgacgg
      241 gaaagcaggc gcgcatcaag gagtcggtgg ctcagcccta cggcgatctg gtgcacgaag
      301 ggcaacattt ggatcccgtg tgccgcgaca tcgaggcact gctcgtgtcc tcccaagcac
      361 gggtttcggg cgaggtcacg gtcctgttcc ggcccgggtc ggtgttcgtc gaaggcgtct
      421 cctcccccta ttcactgatg gcggcatcca agggcgtgta cggcgagtcc gccggtgaat
      481 ggacaccggc cgatgcgctc ggctattcga aaatgctcgc cctgacggga gtgttctaca
      541 agcgcgccgg ctcgcacgcg gcacgggcgg agcaatcatg agcagcatgc gcacggtggt
      601 ggtcgataaa atcgcatcgg tgacgcaggc gtgcggcctg tcgcacgaaa tccgcgtcgc
      661 cgcggacatc ccctgcgacg aaggcgtcgt ggtggtggtc gagatcctca acaacaagtc
      721 cacgtacaac accctcgagt tgaccagcgg ccgcatggcg aagatcgcgc gcggcgacgt
      781 agtggtcggc gcgttgggtc accgcaaagc tctgttcggc tactccggcc atattccgct
      841 ggcgctgaag ccgggcgacg tgattcaaat gttgaatatt ggcggcgtgc tcggcatctg
      901 cgattccgcg acgccggaca agggcaagcc attcgactgc aaggtggtgg gcacggcgct
      961 caccttcccg tatctcggcg aacgcatcgg cgtacccgcg cgcgcgggac acaaggcgct
     1021 cgattacgag gccgcgctcg acgtgcgcgg cgtgccggtc gtggcgctgg ccggcacctg
     1081 catggaagct gggaaaaccg ctgcagcgtc cgccatcatc gcgcgcatgc gccatcgcgg
     1141 tttgattgtg gacgcattca aggcgaccgg cgtgtcactg cgccgcgata ttctcgcgtt
     1201 cgaggattcc ggtgcccgcc gcaccatgat tttcacggac ttaggtgtag tcacgacgac
     1261 cgcgaaatgc ggaccggcgc tgacccggac catgctcacc gaaatgtcgg cgggtcagcc
     1321 ggatgtcatc gtgttcgagc tgggcgacgg gctgttgggg acctacggcg tggagtcgat
     1381 cttgcgccaa ccggacatca aagcggcgtt aacggcggtg gtgctgtcgg ccaatgatcc
     1441 ggtcgccgcc tggggcggcg tcaaattgtt gcgcgagcgt ttcggcatcg aaccggcggt
     1501 ggtcacaggt cccgccaccg acaatcaagt gggggtggag atcatccgcg agcagatgaa
     1561 tgtcgaagcc ttcaatgcca tctcgagccc cgcggacttg ggagaccaca tcatcggccg
     1621 gttgggattg agccacgtgc gcgaatcgaa agtcgccgcc gaatgagcac ggccaccccc
     1681 gccatcgtgc tcggcggcac cggctatgtt gccggcgagc tgctgcgctt gatcgccgga
     1741 catccacaac ttgaagtcgc cgccattctg tccgacagcc agcccggtgc ctcggtcgcc
     1801 gctgcgttcc cgcatctgtg cagttcctat ccggagctca aatttgccga tatcgacgcg
     1861 atcgagacgc tcatcaggcg cacctcgcag agcgccctgt tctcggcggc accgcatggc
     1921 gtggcggcgg ccatcatcga tcgtttattg aacgcggccg gtgccgccgg cactcgcgtg
     1981 cgctgtgtcg atatctcggc cgacttccgc tactcgagcg ccgcagccta cgaggcggtt
     2041 tacaaacacg cgcacggcgc gccggcgcga atccgtgatt tcacctgtgc ggtaccggaa
     2101 catctgcaaa agtcgccgac gccgcacatt gcgcacccgg ggtgttttgc aaccgcaacc
     2161 ttgctcgcca ccgtaccgct gctggcgcta ggtctcaccg ataaccgcct gttcgtgacc
     2221 ggcatcaccg gcagcacggg ttccggtcgc aagccgatcg agggcacaca ccatccgcat
     2281 cggcacagcg atctgtactc ctacggcgcc ttgagtcatc gacatgttcc agagatcatc
     2341 gcctgcgcga agcttgccac gggtgtcgaa cccgaactca atttcgtcgc gcattccggc
     2401 ccgttcgctc gcggcatcca cgtgaccgcg caagccagcc tcaaaaagcc gatcggcacc
     2461 gcgcaggcag tcgaggcgtt caaggcctat tacaagcatt cgccgttcgt tcgcgtgagc
     2521 gaggcggcgc cgcgcgtcaa agatgtggtg agcagcaact atgcgaattt gagtacggtg
     2581 accaatggcg attccatcgc agtcatgtcg gccgtcgaca atctcaataa aggggctgcc
     2641 gggggtgcca ttcaatggat gaatcgacta ctggggctgc ctgagtcgac cggcctcact
     2701 gcgggcgcgc ccggctggac atgaaggcga tgaccgcacc caccgtgatc agccacttgg
     2761 cgcaagtgtt cgcgcaatac cccatcgaag tcgcgcatgg cgagggcgtc tggttgtacg
     2821 cgcgcgacgg gcgcaaggtg ttggatttct acggtggaca cgcggtcgcc ggtttgggct
     2881 atggtcatcc gcgctgggtg gccgcactcg agcgccaggc gcggcagatg gcgtttcaga
     2941 ccaatgcgct gcctatgctg attcgcgaac acgcggcggc ccgcctgacc aaattcgcgg
     3001 gtctcggtct cgacaccgtg ttttggatca acagcggcgc cgaagccaac gagaatgcac
     3061 tgaagctcgc cttcaagctg agcggccgca gcaagatcgt ggcgctcgag cagggctggc
     3121 acggcagaac cgccgccgcg ggagcggtga cttggggcgc cctcgaaaag tggtacggct
     3181 ttcctcgcac gcccttcgat gtgagcttcg caccgcgcga caatccggac gcactcgggc
     3241 aattcatcga ccgccatacc gccgcagtca tcgtcgaacc cgtacagggt gtcggcggcg
     3301 catatgaggt gggtcaacca atgttgcggg cactgcgtca gcgctgcgac gaaaccggcg
     3361 ctctgttgat attcgatgag gtgcaatgcg gcatgggccg cacgggcgcg ccgtttgcag
     3421 cgaattttta cggcgtggtc cccgatatgc tcacctcggc caaggcgttg ggaaacggct
     3481 ttccggtgtc ggcattgctg ttatcgcaac gggtcgcggg gcggatcaag tacgacgata
     3541 tgggtacgac gttcggtggc ggccccatgg cctgtgccgc cgccgaagcg gtcatcgaca
     3601 ccattgaatc agagtcgctg ctcgccaacg tgcggcaggt ttcggcctat atccgcagca
     3661 gctgtatcgt cggtccggtc accggcgtgc aaggcgcggg atttctactg ggcctgaaaa
     3721 catcgcggcc cgccaaggaa gtacacgccg agttgctggc gaagaacata cttgcgggca
     3781 cgagcgcgga tccgcacatc ttgcgcttgc tgcccgcgta catattgaac gaggggcatg
     3841 tcgatcagct gagcgatgcc ctgtcgcgaa tttccgcatg aagcgcttcc tcaagctggc
     3901 ggatttcagc cgcgagcagg ttctcgattt gctggcgctg gctcaggcat tgcaagacca
     3961 gccggattcg cgggcgctcg ccggcaagat cctcggcctt gtgttcatga atccctcgtt
     4021 gcgtaccttg gcatcgtttc aagccgcgat ggcgcgtctc ggcgggtcat gtttcgtgat
     4081 cacgccgggc cagggaacgt ggcaactgga aacgcggccc aacgccacca tgaacggcgc
     4141 ggccgccgaa catattcgcg agggcatccc ggtgctcgcc tcgtattgtg atgcgctcgg
     4201 cgtgcgcgcc ttcgccgaag gcaagaatct cgagagcgat ctgcaggaaa ctttgttcgg
     4261 catgatcgat gctctgtgcg acaagccgct gatcaatttg gaatcggcga tgaatcatcc
     4321 ctgccaggct ctggccgact ggcgcaccct cgatgaactc aaggtgccgc ggcgcgccaa
     4381 gttcgtgctg tcctgggtca atcatccgcg ggcgctgccc ttggcggtgc cggcggcgac
     4441 gctgcacatg gccgcgatgc gcggcatgga agtcgtggtg ctgcggccgc acggcttcgc
     4501 cctgcccgcg ccgatcatgg acggcgcctg cgcggccgcc gccgccgcgg gcggcgcggt
     4561 cagcgaaacc tcggaccgcg cggcagcgct cgccggcgcg catgtgctgt acgcaaagga
     4621 gtggggaagc acgcagcatt acggcgatag cgaagcggat tcgcgcttgc gtgccgagct
     4681 tgccgattgg tgcgtgcgcg agcagtggtt cgcagcggcg gcgcccgatg cgaaactcat
     4741 gcactgtttg cccgtgcgcc gcaatgtcgc ggtcgccgat gaagtcctgg atgggccgcg
     4801 cgccgtcgtc aagcgcgagg cgttcaatcg tctggcggtg cagatggccg ttctacaccg
     4861 catgctgacc tgatcgatac gcgattcgga gtaccgtcat gattatgagc cgacctgatc
     4921 cttccgttgc cgtgcgtgcg ctcaagagcg cggcgccgta tatccgcatg tacaagaaca
     4981 aggtgttcat catcaaggcg ggcggtgcgg ttttcagcga tgagatctcg acgcgcgcgc
     5041 tcatcgaaca ggtggcgatc ctgcaccagg tgggcatcaa gacggtgttg gtgcacggcg
     5101 gcggtccgca gctcgacagc atgcaggcct cgttgggcat cgacacgcgc atggtccatg
     5161 gccggcgcgt caccgaccag aaatcgatcg atgtgaccgc catggtgctg aacggcttga
     5221 tcaacacacg cattctcgcg atctgccgcg aactcgacat cgaggcgatc ggcttgagcg
     5281 gcgtcgacgc cggtttgata cgcgcgcata agcgcccgcc gatgcgcgtc cccgacagct
     5341 ccggagagtt cgtcgattac ggcttcgtcg gcaacatcga ctcggtgaat acgggagtca
     5401 tcgagaaatt gctcgagaac ggcttgatgc cggtggtgag tcctctgtca gccgatgccc
     5461 acggcacgtt gctcaacatc aacgccgaca ccgtggctgc cgccatcggc ggggcgctgt
     5521 cggccgagaa gctcgtgcta tgcaccggcg cccagggaat tcttgaacgt ctggacgatg
     5581 ccagctccgt ggtctcgtat accgacatca agggcttgaa gcgtttgcgc gatcagggcg
     5641 cgctcaaaga cggcatgctg ccgaaggcgg ccgcgatcga gaatgcgatc cgcggcggcg
     5701 tgcgccgtgt gcacgtcatc ggctacaaat ccaccgatag tttgctggcg gagatattca
     5761 ccaacgaggg aaccggcact ctcgtggtcg ccgatttgac cgccttgagc cccgcggaac
     5821 aatcgagcgc tcgatgacgc gtctctggga caagggcatt gcgctcgact cgcaggtgct
     5881 cgcgttcacg gcgggcgatg atcatctgct cgacaatcgg ctggtgagct acgacattcg
     5941 agcatcgatc gcccatgcgg agatgctcgc agttcaggga ctgttgtcgg cgccagactt
     6001 ggaatcgatc cgtgacgcgt tgaccgccat cggcagcgag catgcgcagg gacaatggcg
     6061 gatcgtgctc gagcaagagg actgccaaac ggcgatcgag aatctgctca ccgcccgcgt
     6121 cggcgcggcc ggcggacgct tacacgccgg gcggtcgcgt aacgatcaag tgctcgcggc
     6181 gctgcggctg tacatgcgcg acgccgccac tgagctgcat cagggcgcgg cggcggttgc
     6241 gagtgcgctc gatgcgttgg ccgcgcgcca cggcgccgtg gtgctgcccg gctacaccca
     6301 tatgcaacaa gccatgccga gcagcgtggc gctgtgggcg cagggtttcg ccgcggaaat
     6361 acgcgatgat gccgagggac ttgcactgac gcagcggcgc atcggcaaga accccttggg
     6421 ctcggcggcc ggctacggca cgccgaac
//
LOCUS       DASWZT010000019         4050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_48575, whole genome shotgun sequence.
ACCESSION   DASWZT010000019 DASWZT010000000
VERSION     DASWZT010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48575"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VGE92_00400"
     CDS             complement(<1..534)
                     /locus_tag="VGE92_00400"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY1312300.1"
                     /translation="MNIDLHTHSNCSDGTLTPAALVARAAAAGIEVLALTDHDTVAGL
                     DEADKSAAAHGLRLVPGVEISASWRSQAIHVLGLWIDPAAAEFRRALDTQGERRHERM
                     RKICLRLEKSGLPGMDLLAAVRSHPGVPTRTHLADAMVAGGHVNRSDEAFRKYLGSGK
                     AAHIAAEWPALDVIIGWI"
     gene            complement(531..848)
                     /locus_tag="VGE92_00405"
     CDS             complement(531..848)
                     /locus_tag="VGE92_00405"
                     /inference="COORDINATES: protein motif:HMM:NF023171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3301 domain-containing protein"
                     /protein_id="HEY1312301.1"
                     /translation="MSEDLVLLVLGAGALAGGYRLWQTSMDAREAANRIAKETCSRAV
                     VQFLDGTVAFAGFRWARDDAGRRRLKRTYTFDYTSDGFERAQGFIVLAGLRLEAVGLA
                     GNP"
     gene            923..1309
                     /locus_tag="VGE92_00410"
     CDS             923..1309
                     /locus_tag="VGE92_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312302.1"
                     /translation="MNTNPQPNLTVRVDPHEMVKFVQELMTAAPHGATAPETTMALAQ
                     RQWGGDTPLARAAQLRWSALNRAFGDARVGTWTSHPQRDQIHVPAALIAAAGVAKLTV
                     AEQGVVFDIPTLLDATLELCEPAGHA"
     gene            complement(1306..2061)
                     /gene="cysQ"
                     /locus_tag="VGE92_00415"
     CDS             complement(1306..2061)
                     /gene="cysQ"
                     /locus_tag="VGE92_00415"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEY1312303.1"
                     /translation="MPIVARAGAAIMRIYDAGFTVQHKEDNSPLTLADLESQRVIIEG
                     LSALTPGIPILSEESAQAPWSERRSWRELWVVDPLDGTREFVKRNGEFTINIALVLQH
                     EPVLGVVAAPALKLLYWGARGIGAFSGAPDGPHVPIHISSPKTPLRVVGSRSHASAQT
                     QVYLARLGPHATRGIGSSLKFCLVACGEADLYPRFGPTSEWDTAAGQALLEAAGGQVT
                     RLDGHRLRYNCRESLLNGDFLAFGHASVLAQGS"
     gene            2093..2776
                     /gene="yrfG"
                     /locus_tag="VGE92_00420"
     CDS             2093..2776
                     /gene="yrfG"
                     /locus_tag="VGE92_00420"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF011564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HEY1312304.1"
                     /translation="MGCYHTEMHALPAVDWSAIDTVLLDMDGTLLDLRFDNWFWLDLV
                     PSRYAAAHGMPLADAQAQLAPRFRAVAGTLQWYCIEYWSRELGLDIGSIKREALARIG
                     FLPGALEFLLKLKASGKRRVLVTNSHPVTLAIKNEQVALTQYFDACHSSHPFAYPKEN
                     AAFWPLLEAEVRFECRRTLFIDDSLSVLNAARDYGIGWLRAVRHPDSGRGAQSTGDFI
                     GIDRLSDLM"
     gene            complement(2769..>4050)
                     /locus_tag="VGE92_00425"
     CDS             complement(2769..>4050)
                     /locus_tag="VGE92_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY1312305.1"
                     /translation="TPPGTARPSAPRAIVIAPTRELAVQIHSDALGIGKYSGLRMAIV
                     FGGVDYEKQRRILEEGVDVLIGTPGRIIDYFKQHVFDLRHIQVAVMDEADRMFDLGFI
                     KDIRFILRRLPHPTLRLTMMFSATLSHRVMELAYEHMNNPELIRIEPDKMTVDRVTQV
                     LYFPSTEEKIPLLMGLLRRIDARRTMVFVNTKRVAEVLERTLTANGFVAQALSGDVPQ
                     TKRLKMMRDFHNGEIAVLVATDVASRGLHIPDVSHVFNFDLPNDAEDYVHRIGRTARA
                     GAEGDAISFGCEEYAISLPDIERYIGHQIPRASIDPVDLAVVKAPPPAEWRERAPRQG
                     QSRGGGGGRSGGRHGGPGQRSGGRPGSSGQRSGGAPSGGAPRRSAAPHPAPRPDAGGA
                     APGDAPAQGQQPGEPSRRRRRRGRGSGPAPGPAT"
BASE COUNT          702 a         1334 c         1324 g          690 t
ORIGIN      
        1 gatccagccg atgatcacat cgagcgcggg ccactcggct gcgatatgcg cggccttgcc
       61 ggaacctagg tatttgcgaa aggcctcgtc gctgcgattg acgtgaccgc ccgcaaccat
      121 cgcgtccgcc aggtgcgtgc gggtgggaac acccggatgc gagcgcaccg ccgcgagcag
      181 atccattccc ggcagacccg acttttccag gcgtaggcag atcttgcgca tgcgctcgtg
      241 gcggcgctcg ccttgggtat cgagcgctct gcgaaactcg gcagcggcgg gatcgatcca
      301 cagccccaga acgtgaatcg cctgcgagcg ccaggaggcg gagatttcta cgccggggac
      361 gagtcgcaga ccgtgagcag ccgcactttt gtccgcctcg tcgagacctg ccaccgtgtc
      421 gtggtccgtg agcgccaaga cctcgattcc ggccgcggca gcgcgcgcca cgagtgccgc
      481 cggagtcagg gtgccgtcgg agcagttgga atgcgtatgc aagtcgatgt tcacgggttg
      541 cccgcaaggc ccactgcctc gagccgcaac cccgcgagca cgatgaagcc ctgtgcgcgc
      601 tcgaagccgt cgctggtgta gtcgaacgtg tacgtgcgct tgagccgccg cctgcctgcg
      661 tcgtcgcgcg cccagcgaaa gcccgcaaac gccacggttc cgtccaggaa ctgcaccact
      721 gcgcgcgaac aggtctcctt ggcgatgcgg ttcgcggctt cgcgagcatc catcgaggtt
      781 tgccagagac gatagcctcc cgccaaggcg ccggcgccca aaaccagcag cacaaggtcc
      841 tcgctcatgg cctgaagctt accgcaccgc ggcgcaggaa gcggcccctg gttttccgcc
      901 gtacgcgccc catgtagagc ctatgaacac caacccgcag ccgaatctga ccgtgcgcgt
      961 cgatccccat gagatggtga agttcgtgca ggagctaatg accgcagcgc cgcacggcgc
     1021 caccgccccc gaaacgacca tggccttggc gcagcgccaa tggggcggcg atacccccct
     1081 ggcgcgcgcc gcgcagctgc gctggagtgc gctcaatcgt gccttcggcg acgcgcgggt
     1141 cgggacctgg acctcgcacc cgcagcgcga ccaaattcac gtgccggcgg cgctcattgc
     1201 cgcggccggt gtcgcgaaat tgaccgtggc ggagcagggg gtggttttcg acatcccgac
     1261 actgctggac gcgactctgg agttgtgcga gcccgccgga cacgcctagg agccttgtgc
     1321 caacacgctc gcatggccaa aggccaagaa gtcgccgttg agcaggcttt cgcggcagtt
     1381 gtagcgcagg cggtgaccat cgagccgcgt gacctggccg ccggcggctt cgagcagagc
     1441 ctggcccgcc gcagtgtccc actccgaggt cggtccgaag cgcggataca gatccgcctc
     1501 gccgcaggcc accaagcaga attttaggga gctgccgatc ccgcgcgtcg catgcggccc
     1561 gagccgcgcc agatagacct gggtttgcgc gcttgcatgc gaacggctgc caaccacgcg
     1621 caacggagtc ttcggcgagg atatgtggat cgggacgtgc gggccgtccg gcgcgccgct
     1681 gaacgcgcct atcccccgtg cgccccaata cagcagtttc aaggccggcg cagcgacgac
     1741 ccccaagacc ggttcatgct gcagcaccag cgcgatgttg atggtgaact cgccgttgcg
     1801 cttgacgaac tcgcgcgtgc cgtcgagcgg atcgaccacc cataactcgc gccacgaccg
     1861 gcgctcgctc caaggtgcct gcgccgattc ttcggagaga atcggaatcc ccggggtgag
     1921 cgcgctgagc ccctcgatga tgacccgctg cgattccaaa tccgccaacg tgagcgggga
     1981 attgtcttct ttgtgctgca ccgtgaagcc ggcatcgtaa atgcgcatga tggcggcgcc
     2041 ggcgcgggcg acgatgggca tgagcccgtg agctaacccg aggagatctc gcatgggttg
     2101 ctatcatacc gagatgcacg ctttgcccgc agtggactgg tccgccatcg acacggtgct
     2161 gctcgacatg gacggcacat tgctcgattt acgcttcgac aactggtttt ggctggacct
     2221 cgttcccagc cgctacgccg ccgcccacgg aatgccgctt gctgacgcgc aggcgcagct
     2281 ggcgccgcgc tttcgagcgg tcgcaggaac gctgcagtgg tattgcatcg agtattggag
     2341 ccgggaactg ggattggata tcggcagcat caagcgcgaa gcgctggcac gcatcggctt
     2401 cttgcccggc gcgctcgagt tcctgttgaa gctcaaggcc agcggcaagc ggcgggtgct
     2461 cgtgaccaat tcgcatcccg tgacattggc catcaagaac gagcaagtgg cgctcacgca
     2521 gtattttgac gcatgtcatt cctcgcaccc gttcgcatat ccaaaagaga acgcggcatt
     2581 ttggccgctg ctcgaggccg aggtgcgatt cgaatgccgg cgaaccttgt tcatcgacga
     2641 ctcgctgagt gtgctcaacg cggcgcgcga ttacggtatc ggctggttgc gagcagtccg
     2701 ccatcccgac tccggccggg gcgcgcagtc gacgggcgat ttcatcggaa ttgatcggct
     2761 gtccgacctt atgtagcggg gccgggtgcc ggaccactac cgcggccgcg ccgccgccgg
     2821 cgccgggagg gttctcctgg ttgctggcct tgagccggcg cgtcgccggg tgcggcgccg
     2881 ccggcatcgg gccgcggtgc cgggtgcggc gccgcgctac ggcgcggcgc acccccgctc
     2941 ggggcgccgc cggaacgttg ccccgaactg cccggccggc ccccggaacg ttgacccgga
     3001 ccgccgtgcc tgcccccgga acgcccgccg ccaccgcctc gcgattggcc ttgccgcggc
     3061 gcgcgttcgc gccactcggc cggcggcggc gccttgacga cggccagatc gacgggatcg
     3121 atggaagcgc gcggaatctg atggccgatg tagcgctcga tgtccggcag cgagatggcg
     3181 tattcctcgc acccgaagct gatggcgtcg ccctcggcgc cggcacgagc ggtgcggccg
     3241 atacgatgca catagtcttc cgcatcgttc ggcagatcga aattgaacac gtggctgaca
     3301 tcgggaatat gcagtccgcg cgaggcgaca tcggtggcga ccagcacggc tatctcgccg
     3361 ttgtgaaagt cgcgcatcat cttgagacgc ttggtttggg gtacatcgcc cgacagggct
     3421 tgggcgacga aaccattcgc agtcaacgtg cgctcgagca cttcggccac gcgcttggtg
     3481 ttgacgaaca ccatggtgcg gcgcgcgtcg atgcgcctca gaagtcccat caacaacgga
     3541 attttctctt cggtggacgg gaaataaagc acctgcgtca cccgatcgac cgtcatcttg
     3601 tccggctcga tgcggatcag ctccggattg ttcatgtgct cgtaggcgag ctccattacg
     3661 cggtgcgaca aggtcgccga aaacatcatg gtcaggcgca gcgtcggatg cggcaggcgg
     3721 cgtaaaatga agcggatatc cttgataaat ccgagatcga acatacgatc ggcctcatcc
     3781 atgaccgcta cttggatgtg gcgcaaatcg aagacatgct gtttgaaata atcgatgatg
     3841 cgcccgggcg tgccgatcaa aacatcgacg ccctcttcga ggatgcgccg ctgcttctcg
     3901 tagtcgacgc cgccgaatac gatggccatc ctgagtcccg agtacttgcc tatgcctagc
     3961 gcgtcggagt ggatctgcac cgcgagctcg cgggtcggcg cgatgacaat ggcgcgcggc
     4021 gcgctcgggc gcgccgtgcc gggcggcgtg
//
LOCUS       DASWZT010000020         6283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_53423, whole genome shotgun sequence.
ACCESSION   DASWZT010000020 DASWZT010000000
VERSION     DASWZT010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6283
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53423"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1405
                     /locus_tag="VGE92_00430"
     CDS             <1..1405
                     /locus_tag="VGE92_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312306.1"
                     /translation="PATNAGTVTLNAANGTVQLGLPTDTTQTSLSSSAGVSAGAAGAV
                     TIRGAAIDLGDALVATTVAGSTPSTTRGTIVLDANGGSGALTVANSTLNATTSGATQA
                     GEIDLLGNPIVISNSTISSKTTGTAAAGSICVGSGTACVPAAAVVTSAPRSVIHAPAA
                     SPGSIAISASTLSTSTSTSGNAGNIDVISTGALALSAATIESQSTSPSPTNAGTVGII
                     TLTGAPVSITNHSLVSTTSVGNFVPGETSGGGTAPPATTPGTAITINSTGGVPLQISD
                     SSVNASAGLANGSNIAVNAGGGPIELQGSTLLASANAGNGGNITINDAGNTTLQQSGI
                     LARATAGNGGAINIKLNKGAVFVEDAASLVSATSQTGNNGTVTISGPQTNFQSSLYVP
                     EVSAAKPPELSANACQRDSSHSTFVREGQGGVAPAPDDYVTGRAAGAPAGESAALRGG
                     PTPALAANSPVRLAGCK"
     gene            1402..3189
                     /locus_tag="VGE92_00435"
     CDS             1402..3189
                     /locus_tag="VGE92_00435"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEY1312307.1"
                     /translation="MRAGLVLALGCALWSGLTLAQNVGNGPNYQTPSAPNYKPPTVPP
                     APTYKLPPLPEPSEAQPATGGAQLFVKRIEVQGATAIRPAAIATLTAPYLNRMVSSGE
                     LQSLRIALTHLYVDKGYINSGVILPDQQVKDGVVVFRAIEGKLTRIVVSGKSKLRPRY
                     VASRIRAHVPEPLRIADLQYALRSLQQNPNVLRLDAALQPGDSLGQGVLHLNVEEQPR
                     FSAGLSFDNHQSSSIGADIGTATFGARDLTGYGDEFRGSIGYSHGNTLGSGIFTMPVT
                     AHDAFVQAYYSRADAAIIEQPFRSLNIKEFTRTYGLLYTMPIIDRIENRLVWFAGIES
                     GRQFTELLGAPFSFSPGAQNGVAAVAIALGGLDWTRHGTSSVTDLRVTYRRGVDALGA
                     TIDSANSTALPFNPNPTGADGRFGLEQLQFIHVVRLNGISPWAHLNDRAQLIMRASGQ
                     FSQQPLLSLEKFTVGGVNTVRGDPENLLVRDDGVAATLELQLPLAGYQPVANPRDLVF
                     APFLDYGRSWDKVNANPGNPLLNSTDPDSIASAGLGLLWNPLRGLDAQIYWGRNIATE
                     FHNDDPLHYVPHDLQYHGIHFAVAYVARW"
     gene            3220..4146
                     /locus_tag="VGE92_00440"
     CDS             3220..4146
                     /locus_tag="VGE92_00440"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY1312308.1"
                     /translation="MRIVGLTLLAALAVSAGARAEDCELGQRYLSLAKDRIAAYANDE
                     ALTLLGQSVEACPTYDAYEQLGELAAQSPQREDKTKAVGAFVSAHALAPTPQASAWSL
                     YQYAALLNREGDPQNAYPLIKQARALDSANADIAALSSTIEAEVQHPTQEHILRALHY
                     SLYQPLVAPRVGAMSPAGGSARSVPEPAAPAAASGPSVNIPINFNSGSIVVDPQTQPN
                     VELLAHSLADPSLAGHQFTFVGHSDARGSDQFNVNLSQQRAQAISQLVTNLEPGLKGR
                     IVVEGHGAREPIDMGKDENALRANRRLQVLIK"
     gene            4240..4497
                     /locus_tag="VGE92_00445"
     CDS             4240..4497
                     /locus_tag="VGE92_00445"
                     /inference="COORDINATES: protein motif:HMM:NF045445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312309.1"
                     /translation="MMSIASLRDFLLWCAIVNYAILFGWFLAFIFAHGSLYRLHAKWF
                     RISENQFDVIHYSGMAAYKIAVLTLNVAPYIALRIATGHGI"
     gene            4551..4916
                     /locus_tag="VGE92_00450"
     CDS             4551..4916
                     /locus_tag="VGE92_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312310.1"
                     /translation="MFSYYPLFIALAGFVIAALLSERALSLMQPDAKVALVDASARTR
                     VITILVAAAFVGLIVWRPIIAWAFLGVAYLCLAVRSMFRVQHLNLPVSIARLLHTANL
                     TACGGMLICACIFELRALQ"
     gene            complement(4940..5851)
                     /gene="ppk2"
                     /locus_tag="VGE92_00455"
     CDS             complement(4940..5851)
                     /gene="ppk2"
                     /locus_tag="VGE92_00455"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEY1312311.1"
                     /translation="MHKNKKHSKHHSESTSDGGELRRDVAERVAVEGRWRIVGDGKPP
                     NGVVQKYSYVDELIRLQLELLKMQEWVRLQGLRLCVLFEGRDAAGKGGVIKRITESLN
                     PRVCRVVALGTPTERERTQWYFQRYVQHLPAAGEIVLFDRSWYNRAGVEHVMGFCTEE
                     EYREFLRACPLFEDMLVRSGIYLVKYWFSVNDEEQESRFAERMQNPIKRWKLSPMDLE
                     ARKHWVEYSRAKDEMLEYTDTKRAPWHIVDADNKKRARLNCIAHLLRQIPYKDLQPVI
                     LDLPNRQPDSGYKRPKKSQQRWVKQIY"
     gene            complement(5895..>6283)
                     /locus_tag="VGE92_00460"
     CDS             complement(5895..>6283)
                     /locus_tag="VGE92_00460"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY1312312.1"
                     /translation="AAAAAATAATAAAAVAPAVHADTGGPAPIGKKVPAIIPLPDAAA
                     KLASCPYPEEARRQVETGTVVLLVYVSAEGAAANTQIDSSSGSTSLDQAAVSCVQEFG
                     QFAPHVTGKRATGYWGRMKFNWSFGE"
BASE COUNT         1105 a         2111 c         1941 g         1126 t
ORIGIN      
        1 ccccgccacc aacgcgggca ccgtgacctt gaacgccgcc aacggtacgg tgcagttggg
       61 cctgccgacc gacacgacgc agaccagcct cagctcgtcc gccggtgtat ccgcgggtgc
      121 cgccggcgcg gtgacaatcc gcggcgccgc catcgatctc ggcgatgcgc tggtcgcgac
      181 cacggtcgca ggatccacgc cgagcacgac ccgcggcacg atcgtgctcg acgccaacgg
      241 cggcagcgga gcgctcacgg tcgccaacag cacgctgaat gcaaccacca gcggcgcgac
      301 tcaggccggc gagatcgatt tgctgggtaa cccgatcgtc atcagcaact cgaccatttc
      361 atcgaaaacc acgggcacgg cggcggcggg ctcgatctgc gtcggcagcg gcacggcgtg
      421 cgtacccgcg gccgcggtgg tcacgagcgc tccgcggtcc gtcattcatg cccccgccgc
      481 atcgcccggc agcattgcca tcagcgcaag caccttgagc acctcgacca gcacctccgg
      541 caatgccggg aatatcgacg tcatcagcac gggggcattg gcgctgtcgg ccgctaccat
      601 cgagtcccag tcaacatccc cgtcgccgac caacgccgga acggtcggca tcatcactct
      661 cacgggcgcg ccggtgagca tcacgaacca ctccttggtg tcgactacgt cggtgggcaa
      721 cttcgttccc ggtgaaacga gcggcggggg cacggcgccg ccggccacca caccgggcac
      781 ggcgatcacc atcaacagca cgggcggcgt gccgttgcag atctcggaca gcagcgtgaa
      841 cgcgagcgcc gggctcgcca acggcagcaa catcgcggtc aatgccggcg gcggtccgat
      901 cgagctgcaa ggctcgaccc tgctggcctc ggcgaacgcc ggcaacggcg gcaacatcac
      961 catcaacgat gccggcaaca cgacgctgca acagtcgggc attctggcgc gcgccacggc
     1021 cggcaatggc ggcgcgatca acatcaagct caacaagggg gcggtgttcg tcgaggatgc
     1081 cgcgagcctg gtgagcgcga cttcgcagac cggcaacaac ggtacggtga cgatcagcgg
     1141 gccgcaaacc aacttccagt cttcgctgta cgttcccgag gtgagcgccg ccaagccgcc
     1201 ggaacttagc gccaatgcgt gccagcgcga cagcagccac agcaccttcg tgcgcgaggg
     1261 ccaaggcggc gtggcgccgg cacccgacga ctatgtgacc gggcgtgccg ccggcgcgcc
     1321 cgccggtgag tccgcggccc tacgcggcgg ccccaccccg gctctggcgg ccaattcgcc
     1381 ggtgcggctg gcgggatgca agtgagagcc ggtctcgtct tggcgctcgg ctgcgcgctg
     1441 tggtctggct tgacgctcgc ccagaacgtc ggcaacggtc ccaactacca gacgccgagt
     1501 gcgccgaact acaagccgcc cacggtaccg ccggcaccga cctacaagct gccgccgctg
     1561 ccggagccga gcgaggcgca acccgccacg ggcggcgcgc agctgttcgt caagcgcatc
     1621 gaggtgcaag gcgccacggc catccggccg gccgcgatcg cgacgctgac cgcaccttac
     1681 ttgaaccgca tggtgagcag cggcgagctg cagtcgctgc gcatcgcgct gacgcatctg
     1741 tatgtcgaca agggctacat caattccggc gtcatcctgc ccgaccagca ggtgaaggat
     1801 ggcgtggtcg tgttccgcgc catcgagggc aagctgacgc gcatcgtggt gtcggggaaa
     1861 tccaaactgc ggccgcgcta cgtggcgtcg cgcatcagag cacatgtgcc cgagccgctg
     1921 cgcatcgccg atctgcagta tgcactgcgt tccctgcagc aaaatcccaa cgtactgagg
     1981 ctcgatgccg cgctgcagcc cggcgattcg ttgggccagg gggtcctgca cctgaacgtc
     2041 gaggagcaac cgcgcttcag cgcgggactc tctttcgaca accaccaatc ctcgagcatc
     2101 ggcgcggaca tcggcacggc gacgttcggc gcacgcgatc tgaccggcta cggcgatgaa
     2161 ttccgcggct cgatcggcta ctcgcacggc aatacgctgg gctccggcat tttcaccatg
     2221 ccggtcacgg cccacgatgc cttcgtccag gcctactact cgcgcgccga cgccgcgatc
     2281 atcgaacagc cgttccgctc gctcaacatc aaggaattca cgcgcaccta cggacttttg
     2341 tacaccatgc cgatcatcga ccggatcgag aatcggctgg tgtggttcgc cggaatcgag
     2401 tccgggcgcc agttcacgga gctgctgggg gcgccgtttt cgttctcgcc cggcgcacag
     2461 aacggcgtcg ccgcggtcgc catcgcgctc ggcggcctcg attggacacg ccacggaacg
     2521 agctcggtca ccgacttgcg cgtgacctac cggcgcggcg tcgacgcctt gggcgccacc
     2581 atcgactcag ccaattccac ggctctgccg ttcaacccca atccaacggg cgccgatgga
     2641 cgcttcggtc tcgaacagct gcagttcatt catgtcgtgc ggctgaatgg gatttcgccg
     2701 tgggcgcacc tcaacgaccg cgcccagctg atcatgcgcg cctcgggcca gttcagccaa
     2761 cagccgctgc tgtcgctcga gaaatttacc gtcggcggcg tcaacacggt gcgcggcgat
     2821 ccggagaatc tcttggtacg cgacgacggc gtcgcggcaa cgctcgagct gcagctgccc
     2881 ctggccggct atcaaccggt ggcaaatccg cgcgatctcg tgttcgcgcc gttcctggat
     2941 tacgggcgct cgtgggacaa ggtcaacgcc aaccccggca acccgctgtt gaacagcacc
     3001 gacccggatt ccatcgcgag cgcgggcttg ggcctgctgt ggaatccgct gcgcggactt
     3061 gacgcgcaga tttactgggg tagaaacatc gccacggagt tccacaatga cgatccgttg
     3121 cactatgttc cccacgatct gcagtatcac ggaattcatt ttgccgtcgc ctatgtcgct
     3181 cgctggtagc cgggcacgcg acggagggag gattgcccta tgcgcatagt tggtttgaca
     3241 ctgctggcgg cgcttgccgt cagcgccggc gcccgcgccg aggactgcga actcggccag
     3301 cgctatctgt cgctcgcgaa ggaccggatt gcggcgtacg cgaatgacga ggccttgacg
     3361 ctgctcggac agtcggtcga ggcgtgcccg acgtacgacg cctacgagca gctcggagaa
     3421 ctcgcggcgc aatcgccgca gcgcgaggac aagaccaagg ccgtgggtgc cttcgtgtcg
     3481 gcgcatgccc tggcgcccac tccacaagcg agcgcatggt cgctgtacca atacgcggca
     3541 ctgctcaacc gcgagggcga tccgcaaaac gcctatccgc tcatcaagca ggcgcgggca
     3601 cttgactcag ccaatgccga catcgctgct ttgtcttcca ccatcgaggc agaggtccag
     3661 cacccgactc aggaacacat cctgcgggcg ctgcattatt cgttgtacca gccgttggtg
     3721 gcgccgcgcg ttggcgcgat gtcgcccgcg ggcggcagcg cgcgcagcgt ccccgaaccg
     3781 gccgcccccg ccgccgcgtc gggcccgtcc gtcaacatcc cgatcaattt caactccggc
     3841 agcatcgtgg tcgatccgca aacgcagccg aatgtcgaac tgctcgcgca ttccttggcg
     3901 gatccctcgc tcgccggcca ccagttcacc ttcgtcggac attccgatgc ccgaggcagc
     3961 gatcagttca atgtaaacct ttctcagcag cgcgcccagg cgatcagcca gctggtcacg
     4021 aacctcgagc cggggctcaa gggccgcatc gtggtggagg gccacggtgc gcgcgagccc
     4081 atcgacatgg gcaaggacga gaatgcgctg cgcgccaatc ggcggctgca agtgttgatc
     4141 aagtagcttc ctggtcctcg aactcccggg cgacagaacc cagggtcgcg gtaaaatccc
     4201 gcgtcgcgca atttcaccgc taggcgcctg gagtgattga tgatgagcat cgccagcctt
     4261 cgcgattttc tcctgtggtg cgccatagtg aactacgcga tattgttcgg gtggttcttg
     4321 gctttcatct ttgcgcacgg atcgctctac cggcttcacg caaaatggtt ccggatttca
     4381 gaaaatcagt ttgacgtcat ccattattca ggaatggcgg cgtataagat cgcagtgctg
     4441 acgctgaacg tggcgccata tatcgcactg cgcattgcaa ccggccatgg catctgacaa
     4501 tcgtttggcg cgatcgcggg tcgcgtccac gcgacgtcag gggcggcgcc atgtttagct
     4561 actacccgct tttcattgcg ctcgctggat ttgtcattgc agctctgctc tccgaacggg
     4621 cgttatcgct gatgcaaccc gatgccaagg ttgccctcgt cgacgcttcg gcgcgcactc
     4681 gtgtaatcac tattctggtg gcggccgcgt tcgtggggtt gatcgtttgg cgccccatca
     4741 tcgcgtgggc ctttctggga gtcgcttacc tctgcctcgc cgttcgatcg atgtttcgcg
     4801 ttcaacacct gaacttgccc gtctcgatcg cccgtctcct gcatacggca aacctcacgg
     4861 cttgcggtgg catgctgatt tgcgcatgca tattcgaact gagagccctg cagtgacggt
     4921 cgacggacgc cgtcgcgcct cagtaaatct gcttgaccca gcgttgctgc gatttcttgg
     4981 ggcgcttgta gcctgagtcc ggttggcgat tcggcagatc gagtatcacc ggctgcagat
     5041 ccttgtaggg aatctgccgc agcaaatgcg caatgcagtt gagccgcgcg cgcttcttat
     5101 tgtcggcgtc gacaatgtgc cacggagccc gcttggtatc ggtgtactcg agcatctcgt
     5161 ccttcgcccg cgagtattcc acccagtgct tgcgcgcctc caggtccatc ggactgagct
     5221 tccaacgttt gatggggttt tgcatgcgct cggcgaagcg cgattcctgc tcctcgtcgt
     5281 tgaccgagaa ccaatacttg acgaggtaga tgccggagcg caccagcatg tcttcgaaca
     5341 atgggcaggc gcgcaagaac tcgcggtatt cctcctccgt gcagaagccc atcacgtgtt
     5401 cgacgcccgc gcggttgtac cagctgcggt cgaacagtac gatctccccg gcggccggca
     5461 aatgctgtac gtagcgttgg aaataccatt gcgtgcgctc gcgttcggtc ggcgtgccga
     5521 gtgctaccac gcggcagacg cgcgggttca gcgattcggt gatgcgcttg atgacgccgc
     5581 ctttgcccgc ggcatcgcgc ccttcgaaca gcacgcaaag ccgcagaccc tgcagccgca
     5641 cccactcttg catcttaagg agttcgagct gtaagcggat gagttcgtcg acgtagctgt
     5701 acttctgaac gacgccgttc ggcggcttgc cgtcgccgac aatgcgccat cggccttcga
     5761 ccgcaacccg ctccgcgacg tcgcggcgca gttcgccgcc atcggatgtc gattcggagt
     5821 gatgcttgga atgctttttg ttcttgtgca tggtcacctt ggaccgcgtt gtcttttaaa
     5881 gcgcgagtcc gtgactactc cccgaaagac caattgaact tcatgcgacc ccaatagccg
     5941 gttgcccgct tgccggtcac gtgcggcgcg aattggccga attcctgcac gcagctcacg
     6001 gccgcttgat cgagcgaagt cgagccgctc gaagaatcga tttgcgtgtt cgcagcggcg
     6061 ccctccgcag agacgtacac gagcagcacg acggtgccgg tctccacttg gcggcgtgcc
     6121 tcctcggggt acggacacga ggccaatttg gccgccgcgt ccggcagcgg aataatggcc
     6181 ggcactttct tgccgatcgg cgcaggaccc ccggtgtccg catgaaccgc gggcgcgaca
     6241 gcagcggcag cagtggcagc agtggcagca gcggcagctg cgg
//
LOCUS       DASWZT010000021         5138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_226, whole genome shotgun sequence.
ACCESSION   DASWZT010000021 DASWZT010000000
VERSION     DASWZT010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VGE92_00465"
     CDS             <1..193
                     /locus_tag="VGE92_00465"
                     /inference="COORDINATES: protein motif:HMM:NF020920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312313.1"
                     /translation="DMTVLNDLDRFHLSMDAVDRLPQTGSRGVYLQQQLRDKLIEHKR
                     YIDRYGEDMPEIRDWTWPL"
     gene            312..2477
                     /gene="glgX"
                     /locus_tag="VGE92_00470"
     CDS             312..2477
                     /gene="glgX"
                     /locus_tag="VGE92_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEY1312314.1"
                     /translation="MSGESEISGNYDRSHQVAGWPFPRGANWDGKGVNFSLFSEASEA
                     VELCLFDASGGDERRIRIRERTNGAWHIYVPNVGPGQLYGYRVHGPYVPESGLRFNPN
                     KLLLDPYAKGIGRPLTWADELFGYQLGDPDADLSFDDRDSAPFAPLGVVIDPSFDWSG
                     ERRPAHAAHETLIYEAHVRGMSLRHPDVPSHLRGTYAGMASEPIIKHLQSLGVTAVEL
                     MPTHYFLHDRNLVDKGLRNYWGYNTLGFFAPAPDYASNPDNPRDAIREFKEMVKGLHN
                     AGIEVILDVVYNHTAEGNHAGPTLSFRGIDNMAYYRTVPGDPRHYMDFTGCGNTLNMV
                     HPHSLQLLMDSLRYWVTEMHVDGFRFDLASALARELTDVDQLGAFCDTIYQDPTLAPA
                     KLIAEPWDLGEGGYQVGNFPLGWMEWNGKYRDTLRKFWKGDMGLHSEIATRLAGSADL
                     YETTRRQPSASINFVTAHDGFTLQDLVSYEQKHNAANGEDNRDGSDDNSSWNCGAEGP
                     TDDAQINELRERQKRNFWCSLMFAQGSPMISGGDELSRTQGGNNNGYCQDSEISWYHW
                     DLDAPRTAFLEFAKRVLQYRKRHPNFHRYAFYDSDSEAKHPDKNIVWFRADGKRMVAS
                     DWEDGGWMRTLGMFINGLAPEIRDSEGRCAEDADFLLLLNAYHEPVSFRISHQLHPAG
                     WKVAFDTARPTLPVEQEAVKRNRLVSLAARSFVVLSHER"
     gene            2467..5097
                     /gene="treY"
                     /locus_tag="VGE92_00475"
     CDS             2467..5097
                     /gene="treY"
                     /locus_tag="VGE92_00475"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEY1312315.1"
                     /translation="MSAKWIASYRLQLHAGFPLSQAQRVLPYLTDLGVSHVYLSPCLQ
                     AVPGSQHGYDVTDPTRISEDLGGEAAWSGFVDAAQRLGLGILLDIVPNHMSASHFNPW
                     WDDVLTHGPFSKFAEYFDFRIRPRHPFHVHICSLARPYGAALDAGELKIESLRGRPRV
                     RHYDSTWPLNPASWGALLWSDKKAAGEAAAGDAAAACFGELARLQQIEAPTDQDRHAY
                     ASHAKRATAMLAEAHVSGRLRAAVERAQGDPLVLDAVLQRQFYSLHGWKLAGELGNYR
                     RFFDVGGLSGIRQEIPAVFAASHARVAAMLAAGDIDGVRVDHPDGLREPLEYFKQLRS
                     LLPQGRIYVEKILENDERLKEDWPIDGTVGYEFLAKANRLWMDSRRAETLTATYADFT
                     GHSVNFAALVREKKHAIVESTFVAEHERVSAAALKLARAEPHTRDLSPRQLREALARL
                     IVAMPIYRTYRTLNTLHEDDKRVLLEALQSARISAPEIDPAAFDFLAALLTKSRLNDA
                     EAQFVAQWQQLTPAVMAKGVEDTTFYCFDRLVSCNEVGSQASLIGISANKFHEFCHSL
                     SDRWPNNLLATSTHDNKRSEDVRTRISVLSEIPERWAQALHLWSKMNAAAWQNHAPDR
                     HAEYLLYQTLIGAWPIDVERCWQYMLKACREAKINTSWHEPNQAYEGSLRGFVEGIFK
                     APEFIAALERFVDPLIQPGRINGLAQTLIKMVAPGVPDFYQGTELWDLSLVDPDNRRP
                     VDFEERARLLRVARELNCADVLAEWDSGLPKLWMTARVLALRRERAQDFSDQSKYQPL
                     VAQGAHLSRLLAFRRGENLIAVVPRLTVTLGGEWGDTQLPLPGGRWRNCFTEDALQRE
                     ITPDALFKSFPVALLVREDA"
BASE COUNT          979 a         1531 c         1651 g          977 t
ORIGIN      
        1 cgatatgacc gtgttgaacg atctggatcg atttcatttg agcatggatg cggtggatcg
       61 attgccgcag accggcagcc gcggtgttta tttgcagcag caactgcggg acaagctcat
      121 cgaacacaag cgctacatcg atcgctatgg agaggacatg ccggaaattc gtgactggac
      181 gtggccgctg taacggctga gccgtaaggg atgaggcgaa tgtcctacag cggcttgggc
      241 gtgactccga catgtgccgc agcaacgcaa tcggggcggc gtgccataat gagggcgctt
      301 gagccgttaa cttgagcgga gagtccgaga tttcaggcaa ttacgatcga tctcaccaag
      361 ttgcaggatg gccgtttccg cgcggcgcga actgggacgg caagggcgtc aatttttcac
      421 tgttctcgga ggcgtccgaa gcggtcgagc tgtgtctgtt cgacgcgtcc ggaggcgacg
      481 agcggcgcat ccggatccgc gaacgtacca acggcgcgtg gcacatttac gttcccaatg
      541 tcgggccggg acagctgtac ggttatcgcg tgcatggacc gtacgtgccg gagagcgggc
      601 tgcgcttcaa tccgaacaag ttgctgctgg atccctacgc caagggaatc ggccggccgc
      661 tcacctgggc ggatgaattg ttcggctacc agctggggga tcccgacgct gacctgtcct
      721 tcgacgaccg ggatagcgcg ccattcgccc cgttgggggt ggtcatcgat ccgagcttcg
      781 actggagcgg cgagcggcgt ccggcgcatg cggcgcacga gacactcatc tacgaggcgc
      841 atgtgcgcgg catgagcttg cgccatcccg acgtgccgag ccatttacgc ggcacctatg
      901 cgggcatggc gtcggaaccg atcatcaagc atctacaaag cctgggcgtc accgcggtcg
      961 agctcatgcc gacgcattac ttcctgcacg accgcaatct ggtcgataaa ggtctgcgta
     1021 attactgggg ttacaacacc ctgggattct tcgcgccggc gcccgactat gcgtcgaatc
     1081 cggacaaccc acgcgacgcg atccgcgaat tcaaggagat ggtcaaggga ttgcacaatg
     1141 ccggaatcga agtgatcttg gatgtggtct acaaccacac ggccgaaggc aatcatgccg
     1201 gcccgacctt gtcgtttcgc ggcatcgaca atatggcgta ctaccgtacg gtacccggag
     1261 acccgcgcca ctacatggat tttaccggct gcggcaatac cctcaacatg gtgcatccgc
     1321 attctctgca gctgctcatg gacagtctgc gctactgggt gacggagatg catgtcgatg
     1381 gcttccgctt cgatcttgcc tcggcgctgg cgcgcgaact caccgacgtc gaccaattgg
     1441 gagccttttg cgacacgatt tatcaagatc cgacgctcgc gcccgccaaa ttgattgccg
     1501 aaccttggga tctcggcgag ggcggctatc aagtcggcaa ttttcccctc ggctggatgg
     1561 aatggaacgg caagtaccgc gatacgctgc gtaaattttg gaagggtgac atgggcctgc
     1621 attcggagat cgcgacgcgg ctggccggca gcgcggatct gtacgaaacc acgcgccgtc
     1681 agccgtcggc gagcatcaac ttcgtgacgg cgcacgatgg tttcacgctg caggatctcg
     1741 tcagctacga acagaaacac aacgcggcca acggcgagga caaccgcgac ggcagcgacg
     1801 acaacagctc gtggaactgc ggcgcggaag ggccgaccga cgatgcgcaa atcaacgagc
     1861 tgcgcgaacg gcaaaagcgc aacttctggt gctcattgat gttcgcgcag ggctcgccga
     1921 tgatttcggg cggcgatgaa ttgagccgca cccagggcgg caacaacaac ggctattgcc
     1981 aggactcgga gatttcctgg taccactggg atctggatgc gccgcgcacg gcattcctgg
     2041 aatttgcgaa gcgcgtgctt cagtaccgca aacggcatcc taattttcat cgctatgcgt
     2101 tttacgacag cgactccgaa gccaagcacc ccgacaagaa catcgtctgg ttcagagcgg
     2161 atggcaagcg catggtggcg tccgactggg aggacggcgg ctggatgcga acgctcggca
     2221 tgttcatcaa cggcttggcg ccggagattc gcgacagcga gggacgctgt gccgaagacg
     2281 cggacttcct gttgttgctc aatgcctatc atgaacccgt gtcgtttcgc atttcacacc
     2341 agctgcaccc cgcgggctgg aaggtcgcgt tcgatacggc gcggccgacg ctgcccgtgg
     2401 aacaggaagc ggtgaagcgc aatcgtctgg tgagtttggc cgcgcgctcc ttcgtcgtgc
     2461 tcagtcatga gcgctaaatg gattgcgagt tatcgtctgc agctgcatgc cggattcccc
     2521 ctgagtcagg cgcagcgcgt actgccgtac ttgacggatt tgggagtgag ccacgtatac
     2581 ctgtcgccgt gtctgcaagc ggtgcccggc agccaacacg gctacgatgt gacggacccg
     2641 acgcggatca gcgaggacct cggcggcgaa gcggcgtggt cggggttcgt agacgcggcg
     2701 cagcgtctcg ggctcggcat tctcctcgat atcgtgccga atcacatgtc cgcctcgcac
     2761 ttcaatccct ggtgggacga tgtgctgacg catggaccgt tcagcaaatt cgccgagtac
     2821 ttcgattttc gcatccggcc gcggcacccc ttccatgtgc acatctgctc gctggctcgt
     2881 ccctacggcg cggcgctcga tgccggtgaa ctcaaaatag aatcgctgcg cgggcgtccg
     2941 cgcgtgcgcc attacgacag cacctggccg ctcaatccgg cctcttgggg cgcgctgttg
     3001 tggtcggaca aaaaggcggc cggcgaagcg gccgccggcg atgcggcggc tgcttgcttc
     3061 ggcgagctcg cccggctgca gcagatcgag gcgccgaccg atcaagatcg gcacgcctat
     3121 gcgagccatg cgaaacgggc gacggcgatg ctcgctgaag cgcacgtcag cggtcggttg
     3181 cgcgcggcgg tggagcgcgc gcaaggcgac ccgctggtgc tcgatgccgt gctgcaacgc
     3241 cagttctact cgctgcatgg ctggaaactc gcgggtgaac tcggcaatta tcggcgcttc
     3301 ttcgacgtcg gcggcttgag cgggatccgc caggaaatac ccgcggtgtt cgccgccagc
     3361 catgcgcggg tggcggcgat gcttgccgcc ggtgatatcg acggcgtgcg ggtcgatcac
     3421 ccggacggct tgcgcgagcc gctggagtat ttcaagcagc tgcgctcgtt gctgccgcag
     3481 ggacgaatct acgtcgagaa aatattggag aacgacgaac gcctgaagga ggattggccg
     3541 attgacggta ccgtcggcta cgagttcctg gccaaagcaa atcgcttgtg gatggacagc
     3601 cggcgcgcgg agaccctgac cgccacctat gcggatttta ccggacactc ggtgaacttc
     3661 gcagcgctgg tgcgcgagaa gaagcacgcc atcgtcgaat cgaccttcgt cgccgagcac
     3721 gaacgggtga gcgcagccgc gctcaaactc gcgcgcgccg agccgcatac ccgcgacttg
     3781 agcccgcggc agttgcgcga ggcattggcg cgcctgatcg tcgccatgcc catctaccga
     3841 acttatcgca cgctcaatac gctgcatgag gatgacaagc gcgtgctgct cgaggcgctg
     3901 cagagcgcgc gcatcagcgc gccggaaatc gatcctgcgg cgttcgattt cctggcggcg
     3961 ctgctgacca aatcgcggtt gaacgacgcc gaggcgcagt tcgtcgcgca gtggcagcaa
     4021 ttgacgcctg ccgtcatggc caagggcgtc gaagacacga cgttttactg cttcgatcgg
     4081 ctggtgtctt gcaacgaagt gggttcgcag gcctcgctca tcgggatctc ggccaataag
     4141 tttcacgagt tttgccattc cttgagcgat cggtggccga acaatttgct tgccacctcg
     4201 acgcatgaca acaagcgcag cgaggatgtg cgcacgcgga tttcggtcct gtcggaaatt
     4261 cccgagcgct gggcgcaggc cctgcacctg tggtcgaaaa tgaacgccgc cgcgtggcag
     4321 aatcacgcgc cggatcggca tgccgaatat cttttgtacc aaacactcat cggcgcgtgg
     4381 ccgatcgatg tcgagcgttg ctggcagtac atgctgaagg catgccgtga agcgaagatc
     4441 aacacgtcgt ggcacgagcc caatcaggcg tacgagggtt cgctgcgcgg tttcgtcgaa
     4501 ggaatattca aggcgccgga gttcattgcc gccctggagc gattcgtcga cccgctgatt
     4561 cagccgggcc gtatcaacgg cctggcgcag actctgatca agatggttgc gcccggcgtg
     4621 ccggactttt atcagggcac ggagctatgg gatttgagtc tggtcgaccc cgacaatcgg
     4681 cggccggtgg atttcgagga gcgcgctcgg ctgctgcgcg tcgcgcgcga attgaattgc
     4741 gccgacgttt tggcggagtg ggattcgggc ctccccaaat tgtggatgac cgcgcgtgtg
     4801 ctcgcactgc ggcgcgagcg cgcccaagat ttctccgatc agagcaagta tcagccgttg
     4861 gttgcgcaag gagcacattt gagccgtctg ctggcctttc gccgcggcga gaatctcatc
     4921 gccgtggtgc cgcgactcac cgtgaccctc ggcggcgaat ggggcgacac gcagctgcct
     4981 ctgcccggcg gccgctggcg aaactgtttc accgaggatg ccctgcaacg tgaaatcacg
     5041 ccggacgcgc tgttcaagtc ctttcccgtc gccttgctgg tacgggaaga cgcgtgaagc
     5101 tcgaggtctg ggcaccgtcg gcgcgctcgg tggaactc
//
LOCUS       DASWZT010000022         4083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_58313, whole genome shotgun sequence.
ACCESSION   DASWZT010000022 DASWZT010000000
VERSION     DASWZT010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58313"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VGE92_00480"
     CDS             complement(<1..657)
                     /locus_tag="VGE92_00480"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1312316.1"
                     /translation="MSTPNPAVPNEAAITTALQASRAAREAGRTAESDQLLVRVAQAA
                     PEHPAVLNELGVLMMSRGAAEQAQPLFARATQADPNHAALWSNLANSLRALGRREEER
                     DALEKALALEPRHLTSLLQKGRLFEELGDVRNAARIFQQALASFPAGATPPPAIAEVF
                     EHAKTLIAQDHAALGAALEEKLSEIRARHGGQTQRRVDQCLEMLTGQRTAYFPRPTFM
                     Y"
     gene            759..2744
                     /locus_tag="VGE92_00485"
     CDS             759..2744
                     /locus_tag="VGE92_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY1312317.1"
                     /translation="MTQGSTGEVDLKLIRATSLLDTDPQAAARLASEILAAAPAHSAA
                     LLLMATACRSIGDAAEACAVLERLAAEQPGSAVIQLELARANRAAGRSEPALGALQRA
                     LSIEPDLAEAWAELSSELAAAGNLRGADVAYARYSRLAPDAPWLAESAAALAENRMGS
                     AEQLLKRHLSQSPGDVQAMRMLALVVARRDGFAEAELLLHEALRLAPGFAAARYDLGQ
                     LWMTQQKPAHMPALIDRLLALEPANRDYRNLQAAFLSFIGQHDRSLDILAALIAESPQ
                     NPSAWVNYGHELRAAGRQAESVEAYRRAIELAPTGGAAYWSLANLKTFRFGEAEIAAM
                     RDLLKSPDLPLYDRTTLEFALAKALEDQARYAESFEHYATGNSLRQQSMTYDPASLVA
                     HVQRCKQVFTLEFFAARAGWGLQAADPIFIVGLPRSGSTLQEQILASHSQVEGTRELA
                     DIAAIARELGGIRDGLDPFAYVKSLDKLDRSRLNALAQRYLDDTRIFRPRGLPRFIDK
                     TPNNFLHIGLIQLMFPRASIIDARRNPMACCFSCFKQYFGQGQAFTYDLARLGGYYRN
                     YVELLAHFDSVLPGRVHRVVYEQVVADLPQQVRQVLEYCGLPFEEACLHFYENRRVVQ
                     TASSEQVRRPIFSEGLNQWRHFEPWLGPLKEALGDLV"
     gene            complement(2838..3986)
                     /locus_tag="VGE92_00490"
     CDS             complement(2838..3986)
                     /locus_tag="VGE92_00490"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY1312318.1"
                     /translation="MRVILSSVLAVSCLAAASVQAAGYDETGAWYLAPLAQYDFLDKS
                     RISNDHAGYQIGLGHDFSSNFALELADSNGSFRVRTNFGNPHEKLNAISLDAIFKFLP
                     ATSMLRPYVLVGTGGMTDDIGANTPNNTAWLAEAGGGLLTGLGDQTGSTRWQLRTEVK
                     YRREFIQNTAFIPNNPGDIIASVGFQFMFGAPTPPPPPVAKAAPAVVEEPPPPPPPPP
                     PPEPCHAPAGFQVDANCRIIEQTLVVRAVDFEFNSLRLTEPAQETLDEVATALAKQPE
                     MQVEIQGYTDSIGADAYNLNLSQKRADAVKAYLVSKGLNSTSLTAKGYGKADPIASNA
                     TRDGRAQNRRVAFAVTSAPAHVKVVTKDATDASTEAAKQKDPAATPKK"
BASE COUNT          709 a         1260 c         1320 g          794 t
ORIGIN      
        1 gtacatgaag gtggggcgcg gaaaatacgc ggtgcgttgc ccggttaaca tttccaggca
       61 ttggtcgacg cgacgctgcg tctgcccgcc atggcgcgcg cggatttcgc tcaatttctc
      121 ctcgagcgcg gccccaagcg ccgcatggtc ttgggcaatg agtgtctttg cgtgctcgaa
      181 gacttcggcg atcgccggtg gcggcgttgc gccggccgga aagctcgcca gggcttgctg
      241 gaatatccgc gccgcattgc gcacatcgcc cagctcctcg aacaagcggc ccttttgcaa
      301 cagggaggtc aggtgcctgg gttcgagggc gagcgccttt tccaacgcgt cgcgctcctc
      361 ttcgcgccgg ccgagcgccc gcaggctgtt ggcgagattg gaccacagcg cggcgtgatt
      421 cgggtccgcc tgcgtggccc gtgcgaacag cggctgagcc tgttccgctg cgccccgcga
      481 catcatcagg acgccgagtt cgttgagaac ggcggggtgc tcgggcgccg cttgcgcgac
      541 gcgtaccagc agctgatccg attccgctgt gcggccggct tctcgtgccg cccgcgaggc
      601 ttgcaaggct gtggtgattg cggcttcgtt gggcacagcc ggattagggg tactcacgcc
      661 tcgtattcta ccgcgacagg gcgcgcaatt gacaaaagct caggctgcca ctcatcgtag
      721 ccgcggggca tgggacggcg gccggaggct atgaaaacgt gacgcagggc tcgacgggcg
      781 aagtggattt gaagctgata cgcgcgacgt ccctgttgga tacggacccg caggctgccg
      841 cgcgcctggc gagcgagatt ctcgcggctg cacccgctca ttccgcagcg ctgttgctga
      901 tggcgactgc gtgccgaagc atcggcgatg cggccgaagc gtgtgccgtg ctcgagcgac
      961 tcgcggcaga gcaacccggt tcggcggtca tccagcttga attggcacgc gcgaatcgcg
     1021 ccgccggcag gagcgaaccg gcgctcggcg cgctgcagcg tgcgctttca atagagcctg
     1081 atctcgccga agcgtgggcc gaactgtcga gcgaactcgc ggcggcgggc aatctgcgcg
     1141 gcgccgatgt tgcgtacgcg cgctacagcc gcttggcgcc ggatgcgccg tggttggcgg
     1201 agtcggccgc agcactcgcg gaaaatcgca tgggcagcgc ggagcaactg ctcaagcgcc
     1261 acttgtcgca gtcgccgggg gatgtgcagg ccatgcgcat gttggcgctc gtcgtcgcgc
     1321 gtcgcgacgg gtttgccgaa gccgagctgt tgctgcacga agcgctgcgg ctggcacccg
     1381 ggttcgctgc cgcgcgctat gacctcgggc agctctggat gacccagcaa aagccggcgc
     1441 acatgccggc gttgatcgac cgcctgttgg cgctcgaacc cgcgaaccgc gactaccgca
     1501 acctgcaagc tgcgttctta agcttcatcg gtcaacacga tcgctcgctc gatattctgg
     1561 cggcgctgat cgccgagtcg ccgcagaatc cgtcggcctg ggtcaattat ggtcacgagc
     1621 tgcgcgccgc cggccggcaa gcggaatcgg tcgaggcgta ccggcgcgcg atcgagctgg
     1681 caccgaccgg cggtgcggcg tattggagcc tcgccaactt gaagactttc cgcttcggtg
     1741 aggcggaaat cgcggcgatg agagatctcc tcaagtctcc cgacttgccg ctgtacgatc
     1801 gcaccaccct ggagttcgcg ctcgccaagg cgctcgaaga ccaggcgcgc tatgcggaat
     1861 ccttcgagca ttacgccact ggaaactcac tgcgacagca atccatgacg tatgaccccg
     1921 ccagtctggt cgcacacgtg cagcgctgca aacaggtctt cacactcgaa ttctttgccg
     1981 cccgcgccgg ctggggcttg caggctgccg acccgatatt tattgtcggg ctgccgcgct
     2041 ccggatcgac gttgcaggag caaatactcg ccagtcactc tcaagtcgaa ggcacgcgcg
     2101 agctggcgga tatcgccgca atcgcacgtg agctcggcgg catcagggac gggctggatc
     2161 catttgcata cgtcaagtcg ctcgacaagc tcgacagatc ccggctcaac gcactcgcgc
     2221 agcgctatct cgacgacacg cggatatttc ggccgcgggg attgccgcgg ttcatcgata
     2281 agacgccgaa caatttcctg cacatcgggc tcattcaact gatgtttccg cgcgcctcga
     2341 tcatcgacgc gcgccgcaac cccatggcct gctgcttctc atgcttcaag caatatttcg
     2401 gccagggcca ggcgttcacc tacgatctgg cgaggctcgg cgggtattac cgtaactatg
     2461 tcgaactgct cgcgcacttc gacagcgtgc ttcccggacg cgtgcaccgg gtggtctacg
     2521 aacaagtcgt cgccgatctg ccacagcagg tgcgtcaggt gctcgagtac tgcggactgc
     2581 cgttcgaaga ggcatgtctg catttctacg agaatcggcg cgtcgttcaa acggcgagtt
     2641 cagagcaggt aaggcgcccg atcttttcgg aagggctgaa tcaatggcgc catttcgagc
     2701 cctggctcgg gccgttgaaa gaggccttgg gcgatttggt ctaaggagtc tgcggccggc
     2761 ggatagccgg ctctggttca gagccggcta tccattccat agtccaatca tccggactca
     2821 acgaccgtca gtgccggtca cttcttgggc gtcgccgccg gatccttttg cttggccgct
     2881 tccgtggatg cgtcggtcgc atccttggtg acgaccttga cgtgtgccgg ggcactggtc
     2941 accgcgaaag cgacccgccg gttctgcgcg cggccatcgc gcgtcgcgtt gctcgcgatc
     3001 ggatccgcct tgccatagcc ctttgccgtc aatgacgtcg agttcagtcc tttgctgacg
     3061 aggtatgcct tcaccgcgtc cgctcgcttc tgcgacaggt tcaagttgta ggcgtcggca
     3121 ccaatcgagt cggtgtagcc ttgaatttcg acctgcatct cgggttgttt ggccaaggca
     3181 gtcgcgacct catccaaggt ttcctgcgcg ggctccgtca agcgcagcga gttgaactcg
     3241 aagtccacgg cgcgcacgac cagcgtctgt tcgatgattc ggcaattcgc atcgacctga
     3301 aaacccgcgg gtgcatgaca cggttccgga ggcgggggcg gcggcggtgg gggaggcggc
     3361 tcttcgacca cggccggagc tgccttggca acgggcgggg gcggcggtgt gggggcgcca
     3421 aacatgaact gaaagccgac gcttgcgatg atgtcgccgg ggttattcgg aatgaatgcc
     3481 gtgttctgga tgaactcgcg gcggtacttg acctcggtgc gcagctgcca ccgagtggag
     3541 cccgtttggt cgccgaggcc cgtcaataga ccgccgccgg cttcggccag ccacgcggtg
     3601 ttgttggggg tgttggcgcc gatgtcatcg gtcatgccgc ccgtgccgac gagcacatac
     3661 gggcgcagca tcgaagtggc gggcaggaac ttgaaaatgg cgtcgagcga aatggcgttg
     3721 agcttttcgt gaggattgcc gaaattggtg cggactcgga agctgccgtt actgtcggcg
     3781 agttccagcg cgaaattcga cgagaaatca tgacccaggc cgatctgata gcccgcgtgg
     3841 tcattggaaa tccgcgactt gtccaagaag tcgtactgag ccaacggagc caagtaccac
     3901 gctcccgttt cgtcgtagcc ggccgcctgt acgctagctg cggcgagaca actcaccgcc
     3961 aaaacgctgc tcaaaatcac tcgcataaca atctctccgt gttgaattcg cataaaggca
     4021 actaaaacta gtgtctccag tgcgggacca tttacagttg ttgtttttag actacatacg
     4081 tgc
//
LOCUS       DASWZT010000023         4294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_67976, whole genome shotgun sequence.
ACCESSION   DASWZT010000023 DASWZT010000000
VERSION     DASWZT010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67976"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..271)
                     /locus_tag="VGE92_00495"
     CDS             complement(26..271)
                     /locus_tag="VGE92_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312319.1"
                     /translation="MPIDPTEGNASPDTDDRIRDFWDRHGGPGSRRSEAGDIDPGASG
                     WSEVYAADGYTLRCEWTRIGDRHTMKFTEKPPQAPAA"
     gene            821..1123
                     /locus_tag="VGE92_00500"
     CDS             821..1123
                     /locus_tag="VGE92_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312320.1"
                     /translation="MRHIAKKRAADDFSDLTDGVEDLLQRIADVDSPEVRKIRAKVQI
                     ALTAAKSAWQDTTSYANRTLHRPGEYLRDSPWKTVGIATLLGIGVGALLLRPRADS"
     gene            complement(1140..1634)
                     /locus_tag="VGE92_00505"
     CDS             complement(1140..1634)
                     /locus_tag="VGE92_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY1312321.1"
                     /translation="MNALSTVPDSKADMYRGLARQLSALFEGERDALANAANMCALLY
                     ETLTAVNWAGFYFLKGSQLVLGPFQGKVACVRIPLGKGVCGTAALRRETLIVADVHEF
                     PGHIACDLASRSEIVVPLLQDGKLKGVLDLDSPQLARFDDDDRHGLEAAVALLLQASD
                     FNGA"
     gene            complement(1631..1795)
                     /locus_tag="VGE92_00510"
     CDS             complement(1631..1795)
                     /locus_tag="VGE92_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312322.1"
                     /translation="MNTQISSKFAALAVALLLNAVLVGATAYLFTTRFQQNAAVTNEC
                     AKAMAVFGVV"
     gene            complement(1966..2298)
                     /locus_tag="VGE92_00515"
     CDS             complement(1966..2298)
                     /locus_tag="VGE92_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312323.1"
                     /translation="MSTRISCASARLDDAASLSTFLADIPNSEDLAKVLHLGEAVSSR
                     AYDDETWTARFVASDGVSVKCFAVTDITIDQAEMIAAACVDNPSLDESGFREAVAESL
                     GPTFDPAD"
     gene            complement(2325..2930)
                     /locus_tag="VGE92_00520"
     CDS             complement(2325..2930)
                     /locus_tag="VGE92_00520"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEY1312324.1"
                     /translation="MKRTLLAAVVVVVLGFVAWKLYHAAVASAGRARLAFATLASRTE
                     ARLTVRSPAFGERGDIPFENTQYRGNIFPGLSWTAAPAGTRSYVVIVQDPDAIRNGMP
                     ILHWTLYNIPAGVTQLDAGMTRPPEGASYGPNLAGVNQAYRGPHTPPGRKHQYHWQLF
                     ALDSLLALDAGKSYADLTEGMSGHVIAKGELIGLGQAPPKP"
     gene            complement(2927..3718)
                     /locus_tag="VGE92_00525"
     CDS             complement(2927..3718)
                     /locus_tag="VGE92_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY1312325.1"
                     /translation="MKIVAVTNIKGGVGKTTTAVNLAYLCAAAGRSTLLWDLDAQGAA
                     TYILRCEPNENASAKKLVTGKRELAELPLASGYDKLDVLPSDRSYRNFDVHLSTRKHP
                     TERLLRMSRSLRDVYAALFLDCPPGISLLSENVLRAADAVIVPLLPTPLSVRMLTQLQ
                     EFVTAHEWTDLALLPFFSMVDRRKSLHHDIIASTRELFPRLLATEVPYSSEIERMSVR
                     RAPLPAYSPRSPVGQLYAALWTEIEGRMKLAPPAPAPAPLGEAAV"
     gene            complement(3736..>4294)
                     /locus_tag="VGE92_00530"
     CDS             complement(3736..>4294)
                     /locus_tag="VGE92_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312326.1"
                     /translation="VTELAAAIAADAKRSDTETDAKVREQLATDANSEALACLGHEPQ
                     AAACLYGEAVALGLEARAHPTRAGESLKNMLEELTRAEAADANYDEAGPARVRALVLL
                     RAPGWPLGPGDTEAGLVAARRAVALRPLYPANLLALAEALTKSGDANGARDAYQRARD
                     AAQALPESGDRDQWLHAADQGLQRK"
BASE COUNT          785 a         1391 c         1374 g          744 t
ORIGIN      
        1 tcgccgagcg cagaggaagc agccgctagg ccgccggcgc ctgcggcggt ttctccgtga
       61 atttcatcgt gtggcggtca ccgatccggg tccattcgca gcgcaacgtg tagccatcgg
      121 ccgcatagac ctcggaccag cccgaggcac ccgggtcgat gtcgccggct tcggagcggc
      181 gcgaacctgg gccgccgtga cggtcccaga agtcgcgaat tctatcgtcg gtatcgggtg
      241 acgcattacc ctcggtgggg tcgatcggca ttgaagttct cctagcgcgc atccgcgacg
      301 gcgggtgccg cttgcgcaac cagcgcggca caatcggcgt tctttgccag gcccgggtcc
      361 acgaatgcca agatggcgcc gatcggcgtc agcagcgtgc gccgtgcgct tgcggaagaa
      421 ccccgtcacg accgctagtc gggttgtgag atgacgaggc gcagcagcaa gccgcgcttg
      481 cgggacacgc gcgcaccgct tgcgttcatg aaagcatcat tggccgccgg cgaccgggca
      541 ttgaacgata ggtaccagga caaatcggcg tggcttcgcg caacaaaaaa aaccaccgtg
      601 cgttttgtgc tgaatccaaa catatccgtc tccggctgcg ttcgggcatg ctgcacgccg
      661 ctgctcgaat tctctgtcgg gcattggccc taagcgatgt aggcatggga ggtgtgcggc
      721 gctgtccgtc atttccgaca cggccacgcg ttgcgcgcct attactcgcg ccccggcaac
      781 acgtggacag tagtcacacc ttacaaaccg gagacacagc atgagacaca tcgccaagaa
      841 gcgcgcggcc gatgacttca gcgacttgac cgacggcgtt gaggacttgc tgcagcgaat
      901 cgccgacgtc gacagccccg aggtcagaaa aattcgcgcc aaagtccaga tcgcgctgac
      961 ggcggccaag agcgcgtggc aggacaccac gagctacgcc aaccgcacct tgcatcgacc
     1021 gggcgagtac ttgcgcgatt ctccctggaa aaccgtgggg atcgccacgc tgctcggtat
     1081 cggggtagga gccttgctgc tgcgcccgcg cgccgattcc tgatccatgg cgctggggct
     1141 tacgcgccgt tgaaatccga cgcctgcagc agcaaggcca ccgcggcttc aaggccgtgc
     1201 cgatcgtcgt cgtcgaagcg cgcgagttgc gggctgtcga ggtcgagcac gcccttgagc
     1261 ttcccgtctt gcagcaaggg caccacgatc tcggagcgcg aggccaagtc gcaggcaatg
     1321 tgtccgggga attcatgtac gtcggcaacg atgagggttt cgcggcgcag ggcggccgtg
     1381 ccgcagactc ctttgccgag cgggatgcgc acgcaggcga ctttgccttg gaacggaccg
     1441 agcacgagct gcgatccctt caggaaataa aatccggccc agttgaccgc ggttagagtt
     1501 tcgtacaaga gcgcgcacat gtttgcggcg ttggccaacg cgtcgcgctc gccttcgaac
     1561 agagcgctca actgtcgcgc caatccgcgg tacatgtcgg ctttcgaatc gggcacggtc
     1621 gacagcgcgt tcacaccacc ccgaacaccg ccatcgcctt ggcacactca ttggtcaccg
     1681 ccgcattctg ctgaaagcgg gtagtgaaca ggtacgccgt cgcgccaacg agcacggcgt
     1741 tcagcagcag cgccaccgcg agcgcggcga atttcgatga gatttgagtg ttcatgcagg
     1801 tctcctcagg gtagttcccc aggcagagca aagcaagccg cgtgccagag tcggtctggc
     1861 gctaagtgtc tatttttaaa gacattatct ccgcgccacg gcgctcgaga tgaccgcgga
     1921 cagtgtccgc ggacaaccag cccggacaca tgaacgagat tcatctcagt cggcaggatc
     1981 gaaggtcggc ccaagcgatt ccgcgaccgc ctctctgaac ccgctctcat ccagggacgg
     2041 attatccacg caggccgcgg cgatcatttc ggcttgatcg atcgtgatat cggtcaccgc
     2101 aaagcacttg acgcttaccc catcgctcgc gacgaaacgc gcggtccagg tctcatcgtc
     2161 ataggcacgc gaggaaacgg cctcgcccaa atgcagcacc ttggccaaat cctcgctgtt
     2221 gggaatgtcc gccagaaatg tcgacaggga ggcggcatcg tcgaggcgcg cgctggcaca
     2281 tgaaattctc gtgctcatgc gttgaattgt atatcacgcg gcggctatgg cttgggaggg
     2341 gcttggccca agccgatgag ttcacctttg gcgatcacat gcccggacat accttcagtc
     2401 aaatcggcat agctcttgcc ggcatcgagg gcgagcagcg aatcgagcgc gaacagctgc
     2461 cagtgatact ggtgcttgcg gccaggcggg gtgtgcggac ctcggtacgc ctggttcact
     2521 ccggcgaggt tcggaccgta gctcgcgccc tccggcggcc tcgtcatgcc ggcatcgagt
     2581 tgggtcacgc ccgcaggaat gttgtacaga gtccagtgca gaatcggcat gccgttacgg
     2641 atcgcatcgg gatcctgcac gatgacgaca taggagcgag tgcccgccgg ggccgccgtc
     2701 cacgacaggc cgggaaagat attcccccga tactgcgtat tttcgaaggg gatgtcgccg
     2761 cgctcgccaa aggcgggtga gcgcaccgtg aggcgcgcct cggttcgcga ggccagcgtg
     2821 gcgaacgcca accgcgcgcg ccccgcggag gccaccgccg cgtgatagag cttccaggcg
     2881 acaaacccca acaccaccac gaccacggcc gcgagtaacg tgcgcttcat acggccgcct
     2941 cgccgagcgg cgcaggcgcg ggcgcaggcg gagcgagctt cattcgcccc tcgatctccg
     3001 tccacagggc cgcgtacaat tgtccgaccg ggctgcgcgg cgagtaagcg gggagtggag
     3061 cacggcgcac gctcatgcgt tcgatctcgc tcgaatacgg cacttcggtc gcgagcagcc
     3121 gcggaaatag ctcccgcgtg ctcgcgatga tatcgtggtg cagcgacttg cgccggtcca
     3181 ccatcgagaa aaacggcagc aacgccagat cagtccactc gtgcgccgtc acgaattcct
     3241 gcaactgcgt cagcatgcgc accgacaacg gtgtcggcag cagcggcacg atcacggcgt
     3301 cggccgcgcg cagcacgttc tcggacagca gggaaatccc gggcgggcag tcgaggaaca
     3361 gcgcggcata cacatcgcgc agcgagcggc tcattcgcag cagccgttcc gtcggatgtt
     3421 tgcgcgtgct caagtgcaca tcgaaattgc ggtacgaccg atccgagggc agcacatcga
     3481 gtttgtcgta gccgctcgcc agcggcagtt cggcgagttc gcgcttgccg gtgacaagct
     3541 tcttggcgga ggcgttctca ttcggttcgc agcgcaagat ataggttgcg gctccctgcg
     3601 catccaagtc ccacaacaga gttgaccgcc cggcggcggc gcacagatac gccaagttga
     3661 cggcggtggt ggtcttgccc accccgcctt tgatgttcgt gaccgcgacg attttcatgt
     3721 caagaaacct cgcagctatt tgcgctgtag cccctgatcg gcggcgtgca gccattggtc
     3781 ccggtccccc gactcgggaa gtgcctgcgc ggcgtcgcgg gcgcgctggt acgcatcgcg
     3841 cgcaccgttc gcatcgccgc tcttggtcaa ggcttccgcc agcgccagta aatttgcggg
     3901 gtacagcggg cgcagcgcca cggcgcggcg cgccgcgacc aatccggcct cggtgtcgcc
     3961 ggggccgagc ggccaccccg gtgcccgcag caacaccagc gcacggacgc gggcggggcc
     4021 ggcctcatcg taattggcat ccgctgcttc ggcgcgggtc aattcctcga gcatgttttt
     4081 cagggactcg ccggcgcgcg tcggatgcgc ccgcgcctcg agtccgagtg cgactgcctc
     4141 gccgtagaga caggcggccg cctgtggttc atgcccgagg caggccagcg cctcgctgtt
     4201 ggcgtcggtg gcgagctgct cgcgcacctt cgcatcggtt tccgtgtcgc tgcgctttgc
     4261 atccgcggca atggccgccg ccagttccgt gacc
//
LOCUS       DASWZT010000024         8074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_19614, whole genome shotgun sequence.
ACCESSION   DASWZT010000024 DASWZT010000000
VERSION     DASWZT010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8074
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19614"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VGE92_00535"
     CDS             complement(<1..992)
                     /locus_tag="VGE92_00535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY1312327.1"
                     /translation="MPEAISQTNAPRPSTACEVLVIGGGPGGSTAASLLAQKGYRVVL
                     LEKARHPRFHIGESLLPANLPIMEQLGVADRVRAIGMEKWGAEFVSHRDARSQEFRFA
                     RAWDKSLPLAYQVRRSEFDEILLRRAAQLGAQVIEGCRVRAVDFLDGARGVRVRAEHE
                     DGRSESWTADTVIDASGRDTFLGNQLQAKRRDKKHNSAALYAHFAGARRECGKRAGNI
                     TIYWFDHGWFWSIPLADGATSIGAVVWPYYLKTRNTSLREFFLATIALCPPLAERLRG
                     ATLISNVEATGNYSYACDRSHGANYFLIGDAFSFIDPVFSSGVMLAMNGGVAAA"
     gene            1093..1776
                     /locus_tag="VGE92_00540"
     CDS             1093..1776
                     /locus_tag="VGE92_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008733953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY1312328.1"
                     /translation="MHILIVEDDEVARDYLAKALREVGHTVDVAANGLEGLHLASSLA
                     VDLAIVDRMLPKLDGLALVQSLRATGRKMPVLILSALGDVDDRITGLRAGGDDYLTKP
                     YHMSELLARIDALGRRGENGNAETNTKLKLADLELDRMTRRVMRNGKKIELTAREFQL
                     LEFMMRHSGQVVTRTMLLEGVWDYHFDPQTNVIDVHISRLRQAIDKDFDKPLLHTVRG
                     AGYSLREDE"
     gene            1786..3156
                     /locus_tag="VGE92_00545"
     CDS             1786..3156
                     /locus_tag="VGE92_00545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY1312329.1"
                     /translation="MGPDQRFLGVLDTSPFRIAIVYSLVFACGAAALLYAVDFAVSRV
                     VDGSTDNLIEAEVQGLREQMVVLSRSDFIELIEQRSQDQDVRGAVYLMVDPQLRPVAG
                     NVPAWPKIDPSVGKWFLFKVSVPFGDETREHEMRAQRFPLPGGYKMLVGHDVQERQDV
                     KSLMVRGLIVAVVLTLLLGLGGGFWMGRRILAQAGAISAVATQIMRGDLSQRLPVRDA
                     DDEINTLAREINTMLDKIEQLTLGMRTVLDSAAHDLRTPLNRLQSTAEGAMADLKPGS
                     AERRVLERVTTEVDHMRSTLDALLRIALAETGTVARESVDLSELVASVVELYAPVSEE
                     RGITLESSVAAGAEVFGSRQLLAQALANLLDNAIKFTPDGGHIRVQLRGPPSEPEVIV
                     EDNGPGIPADKREIVLGRRVRLDEARKFPGSGLGLSLVAAVTKLHGARLVLDDAAPGL
                     RVSLKF"
     gene            3328..3876
                     /locus_tag="VGE92_00550"
     CDS             3328..3876
                     /locus_tag="VGE92_00550"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HEY1312330.1"
                     /translation="MAEAADEATAPPPKKSGGGTIVNAVGIFVLTLAAVIAGGFVNAK
                     LHPLPDLQLDKDGKIKAIVPVAAAAHGGEGGEGKAAVYYAIDPPLVVNFEDGSAVRFL
                     QISMEVMARDPKAIESVQKNIPLIRNNLLLLMSNRNYQSMMTREGKEKLRQEALTEIR
                     AVQKKEGGGDVDDLLFTSFVVQ"
     gene            3876..4820
                     /locus_tag="VGE92_00555"
     CDS             3876..4820
                     /locus_tag="VGE92_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HEY1312331.1"
                     /translation="MAETPPNAAISEEEVSALLDKQTADKNAPRPYDFAAQRINRMQL
                     PLLEVVSKSFAERAGASLSALLGRDASMQFIAVESAKSADLQAALPVPASLAVVRLKP
                     LPGNGFVSVEPALLLTLLDGFFGGSGRATPDPQAAIAPAAQRFLALLLKSFAADLTAA
                     WAPVTPLELELVKQETNPRLVQLGAPHESLLVVKFTVEFAARSGRIDWLLPETLIAPI
                     REALAADGGKPSARKQEPWAPAFTAALQDAEVDTRAILAEAQISLRELVRLSPGDIIP
                     IEAPQQVTLFAGDVPLYHGRFGVSQGRNALKILSGASS"
     gene            4817..5164
                     /gene="fliN"
                     /locus_tag="VGE92_00560"
     CDS             4817..5164
                     /gene="fliN"
                     /locus_tag="VGE92_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEY1312332.1"
                     /translation="MSTPDPKNAAVARAQFGDLGPNAAAAAGQEMNLNLILDVAVTLA
                     LEVGRARMSVRDLLQLTPGAVVELDRLAGEPLDVLVNGVRIARGEVVVVNEKFGIRLT
                     DVVSETERMEHAK"
     gene            5161..5523
                     /locus_tag="VGE92_00565"
     CDS             5161..5523
                     /locus_tag="VGE92_00565"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEY1312333.1"
                     /translation="MTAMAQPASPLSVGSLTQLTLSLLAIVALIIVISWALKRLKLAG
                     PRGSGEIAVIDELRVGPRDRIVLIRVGESQVLVGIGASGPVALTPLAAPIVLKGGARA
                     PPFAERLRDLMQRPSGSP"
     gene            5520..6266
                     /gene="fliP"
                     /locus_tag="VGE92_00570"
     CDS             5520..6266
                     /gene="fliP"
                     /locus_tag="VGE92_00570"
                     /inference="COORDINATES: protein motif:HMM:NF009438.0"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEY1312334.1"
                     /translation="MNARRVTASALLVLLPLGAAFAQGALPAVAIKTAADGVQTYSLT
                     FQVLILMTVLTLLPAILLAMTAFTRIIIVLSILRQALGMATTPSNQILTGLALFMTFF
                     VMGTTLDQAYTTGVKPYLDGQLAGEVAIERTLQPLKKFMLAQTRENDLQLFTKLSGKA
                     QFASADDVPLTVLIPTFMTSELKTAFQIGFMVFIPFLVIDLVVASVLMSMGMMMLSPV
                     LVSLPFKIMLFVVVDGWTLLMGTLASSFHL"
     gene            6263..6532
                     /gene="fliQ"
                     /locus_tag="VGE92_00575"
     CDS             6263..6532
                     /gene="fliQ"
                     /locus_tag="VGE92_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEY1312335.1"
                     /translation="MTPENVMDLAHRMLLITSMVAAPLLLTALAAGLVIGMLQAATQI
                     NESTLSFIPKLLLLVLTLLVAGPWILRVLADFTRDLYSSIPAVIG"
     gene            6541..7341
                     /gene="fliR"
                     /locus_tag="VGE92_00580"
     CDS             6541..7341
                     /gene="fliR"
                     /locus_tag="VGE92_00580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEY1312336.1"
                     /translation="MQPVTLNAADVSMWVSRLWWPTLRVGGFVLAAPVASETTIPGFV
                     KIILSLSLAFLMAPLIQTPTELSIFSAAGLLAAVLEVLVGVSIGMVVQLTFEALTFAG
                     QTISLTMGLGFATLVDPQRGANTTVLGQMFMIFGILSYLAINGHLVLLSALAQSFQTL
                     PIGAAQIDKNLLWSVVLWGTRVFETGLLIALPAVIALVIVNLALGVVTRAAPQLNLFG
                     IGFTITLLCGFFVLIAGLGGIMSGISSLIDSALTAVGELVGAPAPGVR"
     gene            7341..>8074
                     /locus_tag="VGE92_00585"
     CDS             7341..>8074
                     /locus_tag="VGE92_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEY1312337.1"
                     /translation="MAAESEQGGERTEEPSQRRLQDARERGQVPRSRELTNFATMIGG
                     SATLIAIGGTLATHLSQIMRHALSIDPARLQDPDTMFSSLSEAAMSATAVLLPVFGAL
                     IGLVVLAAVGLGGWNFSMQAMVPDFTRLSPLAGIKRLLGLRGAAELGKALMKCAVVGG
                     VCAAIVSSIFRDVLALGHMEPRAAIGRGVGLLGWSFVWLSGSLALVAIVDVPLQLFQF
                     KRTLRMTRQELRDEAKESDGRPETKQR"
BASE COUNT         1416 a         2474 c         2671 g         1513 t
ORIGIN      
        1 tcggcggccg cgaccccgcc attcatggcg agcatcacgc cggaagagaa caccggatcg
       61 atgaaactga aagcgtcgcc gataagaaag tagttggcac cgtggctgcg atcacaggcg
      121 taggagtagt tgccggtggc ctcgacgttg gagatgagcg tcgcgccgcg caggcgttcg
      181 gccagcggcg gacacagtgc gatggtggcg agaaaaaatt cacgcagcga ggtgttgcgg
      241 gtcttcaagt aataaggcca gacgaccgcg ccgatgctgg tcgcaccgtc cgcgagcggg
      301 atcgaccaaa accagccgtg atcgaaccag tagatcgtga tattgccggc gcgcttgccg
      361 cactcgcggc gcgcacccgc aaagtgcgcg tataaggcgg cgctgttgtg ttttttatcg
      421 cgccgcttcg cttgcagctg attgccgagg aaggtgtcgc ggcccgacgc atcgatgacg
      481 gtgtccgcgg tccacgattc cgagcggccg tcttcgtgtt cggcgcgcac gcgcacgccg
      541 cgcgccccat ccaggaaatc cacggcgcgg acgcggcagc cttcgatcac ctgcgctccc
      601 agctgcgccg cccggcgcag caggatttcg tcaaattccg agcgccgcac ttggtatgcg
      661 agcggcagcg atttatccca cgcgcgcgcg aagcggaatt cctggctgcg cgcatcgcgg
      721 tgcgaaacga actcggcccc ccatttctcc atgccgatgg cgcgcacccg gtcggctaca
      781 cccagctgct ccataatcgg caagttggcg ggcagcagcg actcgccgat gtgaaagcgc
      841 ggatggcggg ccttctcgag cagcaccacg cggtagcctt tttgcgccag caggctcgcc
      901 gcggtggatc cgcccgggcc gccgccgatc accagcacct cgcaggccgt cgacgggcgg
      961 ggtgcgttgg tttgcgagat cgcctcgggc ataataaatt tggtcttccg atctgctttg
     1021 ggacaacccg cgcgaggttg ttagtctatc gtgccgagaa acacgggtcc aacgtctgta
     1081 agatagccag ccatgcatat cttgatcgtt gaagatgacg aagtggctcg cgattatctg
     1141 gccaaggcgc tgcgcgaggt cgggcacacg gtcgatgtcg ccgcgaacgg actcgaaggg
     1201 ctgcatcttg cgtcgtcgct cgcggtggac ctcgccattg tcgaccgcat gctgccgaaa
     1261 ctcgacggac tcgcgctggt gcaatcgctg cgcgccaccg ggcgcaaaat gccggtgctg
     1321 atattgagcg cgctcggcga cgtcgatgat cgaatcacgg ggctgcgagc cggcggtgat
     1381 gactacctga ccaagcccta ccacatgtcg gaattgctgg cgcgcatcga tgcgctcggc
     1441 cgacgcggcg agaatggcaa cgccgagacc aataccaaac tcaagctggc cgacttggaa
     1501 ctcgaccgca tgacgcgccg cgtgatgcgc aacggcaaga aaatcgaact caccgcccgg
     1561 gagtttcagc tgctggagtt catgatgcgc cacagcggcc aggtggtcac gcgcaccatg
     1621 ctgctcgagg gcgtgtggga ctatcacttc gacccgcaga ccaacgtgat cgacgtgcac
     1681 atcagccgtt tgcgccaggc gatcgacaag gattttgaca aacccctctt gcacaccgtg
     1741 cgcggtgcgg ggtacagctt gcgcgaagac gaatagcggt ccgccgtggg ccccgatcaa
     1801 cggtttctcg gcgtactcga cacgtcgccg ttccggatcg cgatcgtcta ttccctggta
     1861 ttcgcgtgcg gcgccgccgc gctgttgtac gcagtcgatt ttgccgtgtc gcgggtcgtc
     1921 gacggcagca ccgacaatct gatcgaggcc gaagtgcagg gattgcgcga gcagatggtg
     1981 gtgctgtcgc gcagcgattt catcgagctg atcgaacaac gctcgcagga ccaggacgtg
     2041 cgcggcgcag tgtacctgat ggtggatccg cagctgcggc cggtcgcggg caacgtcccg
     2101 gcgtggccga agatcgatcc cagcgtgggc aagtggtttc tgttcaaggt atcggtgccg
     2161 ttcggcgatg agacgcgcga gcatgaaatg cgcgcgcagc gctttccgtt gcccggcggc
     2221 tataaaatgt tggtgggaca cgacgtccag gagcggcaag acgtcaagag tctgatggtg
     2281 cgcggcttga tcgtggccgt ggtgctgact ttgctgttgg gtttgggcgg cggcttttgg
     2341 atgggccggc gcattctggc gcaggccggc gcgatatcgg cggtggcgac gcagatcatg
     2401 cgcggcgatt tgtcgcagcg gttgccggtg cgcgatgcgg acgatgaaat caacaccttg
     2461 gcgcgggaga tcaacaccat gctcgataag atcgagcaac tcacgctcgg catgcgcacg
     2521 gtgctcgaca gcgccgcgca tgatttgcgc accccgctga atcgactgca atcgaccgcc
     2581 gagggtgcca tggcggatct caaacccggg tcggccgagc ggcgtgttct ggaacgggtc
     2641 acaaccgaag tcgatcacat gcgcagcacc ttggacgcgc tgctgcgaat cgcgctcgcc
     2701 gagaccggca ccgtggcgcg agaatcggtc gacctgtccg aattggtggc gagcgtggtc
     2761 gagctgtacg cgcccgtcag cgaagagcgc gggattacct tggagagttc ggtggccgcg
     2821 ggggcggagg tgttcggcag ccgccagcta ttggcccagg cgctggcgaa tttgctcgac
     2881 aatgccatca aattcactcc cgacggaggc catatccgcg tgcaactgcg cgggccgccc
     2941 agtgagcccg aggtgatcgt cgaagacaat ggacccggga tccccgcgga caagcgcgag
     3001 atcgtgctcg ggcgccgggt gcgcttggat gaggcgcgta agtttcccgg gtccgggctc
     3061 ggattatcgc tggtggcggc ggtcacgaag ctgcacggtg cgcgtctcgt gctcgacgac
     3121 gcggcaccgg gcttgcgcgt ctcgctgaag ttctaaagca agcggaaccg tcaatttctc
     3181 gacggatggc gccgaggccg atgcggcgag ggacgccgaa ctcgtcctaa gctactgaat
     3241 tatctgagct aagacaacca gcatggctct ggcatagaac ttgctcaatt cctggtttgc
     3301 ggcatgtgac cgcgctagga atcaaccatg gccgaagcag ccgatgaagc tacagccccg
     3361 ccgccgaaga aatccggcgg cgggaccatt gtcaacgccg tcggcatctt cgtgctgacg
     3421 ctggcggccg ttatcgccgg cggcttcgtc aacgcgaagc tgcatcccct tcccgatctg
     3481 caattggaca aggacggcaa gatcaaggca atcgtcccgg tcgcagccgc cgcccacggc
     3541 ggtgagggcg gggagggtaa ggcggccgtt tactatgcca tcgacccccc cttggtggtg
     3601 aatttcgaag atggctcggc ggtgcgcttc ttacagattt cgatggaagt gatggcgcgc
     3661 gatccgaagg ccatcgaaag cgtgcagaag aacatcccgc tgatacgcaa caacttgctg
     3721 ctgctgatga gcaatcgcaa ctatcagtcg atgatgacgc gcgagggcaa ggagaagctg
     3781 cggcaggaag cgctcaccga gatacgcgcc gtgcagaaaa aggaaggcgg cggcgatgtc
     3841 gacgacctgc tgttcaccag cttcgtggtg cagtaatggc agaaacgccg cccaatgccg
     3901 ccatctccga ggaagaagtg tcggcgctgc tcgacaagca gaccgcggat aagaacgcgc
     3961 cgcgccccta tgatttcgcc gcgcaacgca tcaatcgcat gcagttgccg ctgctcgagg
     4021 tggtcagcaa gagcttcgcc gagcgggccg gcgcgtcgct gtcggcgcta ttgggccgcg
     4081 acgccagcat gcagttcatt gcggtggaat cggccaagag cgccgatttg caagccgcgc
     4141 tgccggtacc ggcgagcctc gccgtcgtac gcttgaagcc gctgcccgga aacggcttcg
     4201 tgagcgtcga acccgcgctg ttgttgacgc tgctcgacgg cttcttcggc gggtcgggcc
     4261 gcgccacccc cgacccgcaa gcggcgatcg caccggccgc gcaacgcttc ctggcgttgc
     4321 tcctgaagag tttcgcggcc gacctgacgg cggcgtgggc acccgtgacc ccgctcgaac
     4381 tggaactcgt caaacaggag accaatccgc gcttggtgca attaggcgcc ccgcacgagt
     4441 cgctcctggt ggtcaaattc acggtggaat tcgcagcccg cagcggacgg atcgattggc
     4501 tgctgcccga gacgctgatt gcgccgatac gcgaagctct ggccgcggac ggcggcaagc
     4561 cgtccgcgcg caaacaggag ccgtgggcac cggccttcac cgcggcattg caagacgccg
     4621 aggtcgatac ccgcgcgatt ctcgcggaag cgcaaatcag cctgcgtgaa ttggtgcgcc
     4681 tgtcgcccgg cgacatcatt ccgatcgaag cgccgcagca agtgacgttg ttcgccggcg
     4741 atgtcccgct gtatcacggc cgtttcggcg tctcgcaggg acgcaatgct ctcaagattc
     4801 tttcaggagc ctcctcatga gcacgcccga tccgaaaaac gccgccgtag cgcgcgcgca
     4861 gttcggcgat ttaggtccca atgccgccgc tgcggccggt caggaaatga acctgaatct
     4921 gattctcgac gtggccgtga cgttggcgct cgaagtgggg cgggcgcgca tgtcggtgcg
     4981 cgatctattg cagctgacgc ccggcgccgt cgtcgaactc gaccggcttg ccggcgaacc
     5041 gctcgatgta ttggtgaacg gcgtgcgcat tgcgcgcggc gaagtagtgg tggtcaacga
     5101 gaaattcggc atccgactta cggatgtggt gagcgaaacg gaaaggatgg agcacgccaa
     5161 atgacggcga tggcgcaacc cgcctcgccc ctctcggtgg gcagcttgac gcagctcacg
     5221 ctcagtctcc tggccatcgt cgcattgatc atcgtcatca gttgggccct gaagcgcttg
     5281 aaattggcgg gcccgcgcgg ctccggggag atcgcggtga tcgacgagct ccgcgtcggc
     5341 ccgcgcgacc gcatcgtctt gattcgcgtc ggcgaatcgc aagtgctggt gggcatcggc
     5401 gcgtcggggc cggtcgccct gacgccgctc gctgcgccga tcgtactcaa aggcggcgcg
     5461 cgcgcgccgc cgttcgccga gcgactgcgg gatttgatgc agcggccgag cggttcgccg
     5521 tgaacgcacg acgcgtgacg gcgagcgcgc tgttggtgtt gctgccgctc ggcgcggcgt
     5581 tcgcccaggg cgcgctgccg gccgtcgcca tcaaaaccgc cgccgacggc gtgcagacgt
     5641 actcgctcac gtttcaggta ctgatcctga tgacggtgct gacgttgctg ccggcaatcc
     5701 tgctggcgat gacggcgttc acccgaatca tcatcgtgct gtcgattctg cggcaggcgc
     5761 tcggtatggc gaccaccccg tcgaaccaga tactgaccgg cctggcgctg ttcatgacct
     5821 ttttcgtgat gggcaccacc ctcgatcagg cgtatacgac gggcgtcaag ccgtacctcg
     5881 acggccagtt ggcgggcgaa gtggccatcg agcgcacctt gcagccgctc aagaaattca
     5941 tgctggcaca gacccgcgaa aacgacttgc agctcttcac caagctctcc ggaaaagcgc
     6001 aatttgccag cgccgacgat gttccgctga cggtgctgat tccgaccttc atgaccagcg
     6061 agctgaaaac cgcgttccag atcggcttca tggtattcat tcccttcttg gtgatcgacc
     6121 tggtggtcgc cagcgtgttg atgtcgatgg gcatgatgat gctgtcgccg gtgctcgtat
     6181 ccctgccatt caaaatcatg ctgttcgtgg tggtggatgg ctggacgctg ttgatgggca
     6241 cgctcgcctc gagttttcac ctgtgacgcc cgaaaacgtc atggatttgg cgcaccgcat
     6301 gctgctcatc acttcgatgg tcgccgcgcc gctgctcctg accgcgctgg ccgcgggttt
     6361 ggtgatcggc atgctgcagg cggcgacgca aatcaacgaa tcgacattga gcttcattcc
     6421 gaaattattg ctgttggtat tgacgttgct ggtggccggg ccgtggattc tgcgcgtgct
     6481 cgccgatttc acgcgcgacc tgtattccag catcccggca gtgatcggct aaacgcccgc
     6541 atgcaacccg tgactctcaa tgcagccgat gtatccatgt gggtgagccg cctgtggtgg
     6601 ccgaccttgc gcgtcggtgg attcgtgctg gccgcaccgg ttgcgagcga gacgacgata
     6661 ccggggttcg tcaaaatcat cctgtcgttg tcgctggcgt tcttgatggc gccgctgata
     6721 caaaccccga ccgagctgtc gattttctca gccgcggggc tgctggcggc ggtattggag
     6781 gtactggtcg gggtgtccat cggcatggtg gtgcaactga ccttcgaggc gctgaccttc
     6841 gccggacaga ccatctcgct gaccatgggg ttggggtttg caaccttggt ggatccgcag
     6901 cgcggcgcga ataccaccgt gctcggtcag atgttcatga tattcggcat cctctcgtat
     6961 ctcgcgatca acgggcatct ggtgctgctc agcgcgctcg cgcagagctt tcagacactg
     7021 ccgatcggcg ccgcgcagat cgacaagaat ttgctgtggt cggtggtgct gtgggggacg
     7081 cgtgtttttg agacggggct gctgatcgcg ctgccggcgg tcatcgcgct cgtgatcgtc
     7141 aatctggcgc tcggcgtggt cacgcgcgcg gcgccgcagc tgaacctgtt cggcatcggc
     7201 ttcaccatca cgctgctgtg cggattcttc gtgctgatcg ccggcctcgg cggcatcatg
     7261 tcgggtattt ccagcctcat tgacagcgcg ctgacggccg tcggcgaact ggtcggcgca
     7321 ccggcgccgg gtgttcgctg atggccgccg aaagcgagca aggcggcgaa cgcaccgaag
     7381 aaccttcgca acgccgtctg caagatgcgc gcgagcgcgg ccaagtaccg cgctcgcgcg
     7441 aactcaccaa tttcgcaacc atgatcggcg gcagcgcgac cctgatcgcg atcggcggca
     7501 cgctggccac gcacctgtcg cagatcatgc gtcacgcact cagcatcgat ccggcgcggc
     7561 tgcaagatcc cgacacgatg ttcagttcgc tgagcgaggc cgcgatgagc gcgaccgccg
     7621 tgctgctgcc ggtgttcggc gcgttgatcg gcttggtagt gttggcggcg gtcgggctcg
     7681 gcggctggaa tttcagcatg caagcgatgg tgccggattt cacgcggttg agtccgctgg
     7741 ccggaatcaa gcggctgttg ggtctgcgcg gcgccgcgga actcggcaag gcgctcatga
     7801 aatgcgcggt cgtcggcggc gtttgcgcgg ccatcgtgtc gtcgattttc cgcgatgtgc
     7861 tggcgctcgg gcacatggag ccgcgcgccg ccatcggccg tggcgtggga cttctcggct
     7921 ggtcgttcgt ctggctgagc ggttcgctgg cgctcgtcgc catcgtcgat gttccgctgc
     7981 aactgtttca gttcaagcgc acgttgcgta tgacacgcca ggaattgcgc gacgaagcca
     8041 aagagtcgga cgggcgcccg gagacgaagc agcg
//
LOCUS       DASWZT010000025        32017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_82498, whole genome shotgun sequence.
ACCESSION   DASWZT010000025 DASWZT010000000
VERSION     DASWZT010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 32017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32017
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82498"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VGE92_00590"
     CDS             complement(<1..494)
                     /locus_tag="VGE92_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY1312338.1"
                     /translation="MCHRSRINLCALLLLLGALDLAARGAAADEASRGLSLLVLGSGG
                     PGATGRAGAGYLVMLDGTPRILVDAGPGTFVRAGEAGADLSRLDIVLLTHLHVDHAAE
                     LAGLIKARIVSERSDIEFAIFGPAGSPGGHGNARFPSTTQFVDLMFGPHGAFSYLSDF
                     AGHMT"
     gene            complement(494..1942)
                     /locus_tag="VGE92_00595"
     CDS             complement(494..1942)
                     /locus_tag="VGE92_00595"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY1312339.1"
                     /translation="MASFPSYDVIIVGSGAGGAAAAYRLALRGVKVLLLEKGAPLPHD
                     GSTLDDRRVVTHGEFLSKEPWIDGTGRPICPQEHFNLGGKTKWYGAAVLRYSGSEFAA
                     DPAYSARGWPIGAAEFAPYYDEAERLLAVRTFSCEPGLKLILERLGASDRSWQSTPLP
                     LALSADILRHPEEATHFDGFASAANLKGDAEENFLRPVADRPNLTIRTSAEVAELLPA
                     RSDAARIGGVKLMNGEELRAPAVLLAAGALHSPRLLLRYLAAHAGRSDFPTLGAVGRN
                     LKKHVLTALVAVSVSPQSDLLRKTMITTHTEHPHSSVQPLGFDAEMIATLIPRFVPRL
                     VARIIGRHSYGFFLQTEDGSHPDNRVLERGDPPERVMDYDALRTPASQAEHRSFTRAF
                     KRALLRIGFISLTQRIGLDGTAHIDGSLIAGTDPNTSVVAANGAVFGIEGLYVVDGSV
                     LPRSSRVNPSLTIYAWGLRVAELLADSLEAAR"
     gene            complement(2078..4156)
                     /locus_tag="VGE92_00600"
     CDS             complement(2078..4156)
                     /locus_tag="VGE92_00600"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY1312340.1"
                     /translation="MPFSNYTSRSLNGLRVGLSSLAVLQLALGAPLASAAAVVADHSA
                     QTRTPIKHVIVIIGENRSFDHVFATYEPKNGENVWNLLSEGIIKQDGSPGPNFYKAQQ
                     HAAADPAADAFLLNPAKTTFPNGVLPAPLVGGPKDSYVTGDNLALAQQSENGLPEDYY
                     QYLISGGTGQTSHTPDQRIAHVSALPAGPFQLTNGRTFDYNAYAASPVHRFYQMWQQL
                     DCSVEHATPEIPSGCDSKLFSWVEVTVGAGTNGLPQPANFSTEYSAGAKTTGEGSTAL
                     GFYNVQQGDAPYFKSLADDYAMSDNFHQSVNGGTGANHIMFGHGDAIWFSDGAGHALP
                     PPHDVEVAKGTANAGVVDEVENPNPAPGTNNWYSEDGYGGGSFGSASYGGGSYSECAN
                     SQAPGVAEVVKYLQSLPRKIDPRCEAGHYYLLNNYNPGYFGNGNNAFTDTNANNTVFT
                     IPPSSTPSIGDSLLEKGVSWKYYGDQWNNYVPDPYQLNYGAMGANTDEYCNICNPFQY
                     DTSIMANPKIRAAHIQDTADLYSDIAKGTLPAVSIVKPSGLVDGHPSSSKLDLFEGFS
                     KKIVDAVKANPELWKDTAIFITFDEGGGYYDSGYVQPVDFFGDGTRIPLLVVSRYTKP
                     GHISHDYSDHVSILKFIERNWSMHPVTHRSRDNFPNPVAARHNPYVPVNSPAISDLFE
                     LFDFRDTWNR"
     gene            complement(4268..6034)
                     /locus_tag="VGE92_00605"
     CDS             complement(4268..6034)
                     /locus_tag="VGE92_00605"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEY1312341.1"
                     /translation="MKTSSRPHTVAGVANRARLGWLMLAACAALPCAARAAGADPKEV
                     CPRPPPGSVIPEPKDLRSQNGVLSLDLTVHNARQSDGSVRYCYVLGDGSQAPTLRVNP
                     GDLLVLHLKNALTDLDGAASPAHQHGVAARSENPCSSARMSLTATNLHFHGMTVPPVC
                     HQDEVLKTSIQPGDPPFEYRIRVPASEPPGLYWYHPHIHGFSKAQVLGGASGALIVEG
                     IERANPELAGLPERVLIVRDQDLLNPDAPPSQSEPVVPKMFIDRDGDSANNGTGFGKP
                     AKDLSVNFVPVPYPDYPPASLQMRPGERQLWRVLNASAITYLNLALLFNRASQQLGIV
                     AIDGVPLNQNGNPANAIQWTDHIGVPPGARVEFIVTGPPAGVPALLVTRTVDTGQGGE
                     NDPNRALAAVIAASDAPEPSARLSAAPKALPPAGLPWLGKVAPVRVRKLYFSEKLSDA
                     HDPNSATEFYLTVDGQTPLAFDPQSDIPNIVAHQGDVEDWIIENRSAELHAFHIHQIH
                     FLLLDWSGTPVNEPFLRDTVNVPYFNGRMLRYPSVRLRMDFRDPNTIGTFVYHCHLLE
                     HEDGGMMGLIRVEAAAHATAAR"
     gene            complement(6060..8318)
                     /locus_tag="VGE92_00610"
     CDS             complement(6060..8318)
                     /locus_tag="VGE92_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1312342.1"
                     /translation="MTHQRCLRPARAACSGLLCAAAGINALCLAVACAEPAPSVAAST
                     DFPLNEITVTAQRLGLLGTAGSASEGIVTDEEIQLTPAYRPGQLLETVPGLIATLHSG
                     EGKANQYLLRGYNLDHGTDLETYVDSMPVNQPTHAHGQGYTDMNFMIPELADGIAYTK
                     GPYYANVGDFGAVGSVRISYRDTIPDQVSATLGTYGFQRVFAAGSTPLGDGHLLAGIE
                     GQHYDGPFDNPDDARKGNFVLRYSQGDQTNGYSLTGMYYHQLWTNTTDIPIRTIAEGL
                     VPDRFGTLDPTDGGRAQRASVAAEFHDAIGNGQLTASTFYIRNDLNLYNDFTHYLRDP
                     LYGDQEDQFERRDAGGGALSYRFALTSFLAPSELLVGGLTRYDALRVGRAPSLAMSDL
                     PASTAPPSFSDLDQVYLFAGAAFVQATTRWTSKFRSVLGFREDYQHGTDIDYLAALHE
                     TVGYSNAGTVHQGLAQPKGSLIYTPNDALEFYLSAGRGFHSADIRGVNQDTSVDLGLP
                     RTSLLAKQEGQEVGLRAAPARNVTFTLAVYNLWQQSETIIDPDVGADSAGPPSRRYGY
                     EVNLTYKINQWLEFYGSYSGDHTRFTRDFDDGTGHLGRYITDAPVASGSLALYLTNLG
                     PWSGGLELRYLGDYALSSGPCVDSAAVHDFPGVATSCANAPTAAGQVNGKGFAQLNLD
                     AAYALPSNWSASLGIYNILNSHAAAAEFWYVDRLQNEIGTYPDGRADMHEHPLEPIMA
                     RLTITKKFGAAN"
     gene            8628..10289
                     /locus_tag="VGE92_00615"
     CDS             8628..10289
                     /locus_tag="VGE92_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015486861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance system multicopper oxidase"
                     /protein_id="HEY1312343.1"
                     /translation="MRNHADRGRRRFIQSLGLGGAAAAFGLLPCASRSRAATSAAPLS
                     GTEFDLTLSEIRLNVRGESRPATAVNGGVPGPTLHWREGTTVTLRVHNALRTPSSIHW
                     HGIVLPFEMDGVPGISFEGIAPGETFTYRFPVRQSGTYWYHSHTRFQQQTGLYGAIVI
                     EPAGGEPIRAHRDHVLLLSDWTDEDPEHVYTALKQMSDFYNFQLPSVADFARDSRSMG
                     VREALAKRRMWNEMRMNPTDLGDVSGATYTYLVNGASAAANWTALAMPGERVRLRLIN
                     GSSSTFFDVRIPGLPMTVISADGQAVDPVTVEEIRIGAAESYDVLIEPTEDRACTIFA
                     QSMDRTGFARATLAPRAGMEAAVPELDPKSWLSAGDLGLRDMSGMSGMSMSGARSSNP
                     SIDMRVPQPSRALDDPGPRLRGNGRRELTYADLHTIGGSRDPRAATREIQLRLTGNMQ
                     RFIWGFDGKKFSEADAVHFDRGERLRIVLSNDTIMAHPIHLHGMFGELEAANGDVLVR
                     KHTFIVQPGKQLSYLVTADNPGQWAYHCHLLYHMMAGMFRQVVVA"
     gene            10286..11089
                     /locus_tag="VGE92_00620"
     CDS             10286..11089
                     /locus_tag="VGE92_00620"
                     /inference="COORDINATES: protein motif:HMM:NF017119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HEY1312344.1"
                     /translation="MRRAGRAALGAFCWLACSGAALADVPEGAIAHAPDAALERPAAS
                     QQVTPSMMPGMDMDDATNQHFVWLENLEAVAGTVHGGAWDAQAWFGGDLHKLWLKSEG
                     DSLDGRNQQAKLEALWDHAVLPLWDTQLGWRHDFGPGPSRDWAAFGVQGLTPYWFDAE
                     ITGYVGEQGRTAARLKTEYDLFITQRLVLKPEIELNAYGKPDRERALGAGLAQGQFEL
                     RLRYAFTRRVAPYLGYVYEVKFAQSASLARAAGAPALEHRAVAGVAFLF"
     gene            11105..12265
                     /locus_tag="VGE92_00625"
     CDS             11105..12265
                     /locus_tag="VGE92_00625"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY1312345.1"
                     /translation="MNSKAPPLAGMTVLDFSHALAGPYSTMLMAVYGARVIKIESPEG
                     GDIGRAWGPPFQGDDAAYFVGINSGKQSVAIDLKSSAGRDICRNLALQADIVVENFRP
                     GTMQRLGLCFESLSAINPRLIYVSISGYGQTGPRTLEPAMDLIIQSASGLMSITGTTA
                     GETVKTGHSIVDISAGLFALIGALLAIEARHRTGLGQLVDVSMMDAVMSTMIANFARY
                     LASGVIPGPMGTLFESIVPYRNYRCADREITIAVASDKLWQHFCEAIGRADLSTHPDY
                     NANALRVRNRATLEPILEAIFRSESAAHWVAQLSKHGVPATLVRNLKEVIDDQQTAAR
                     DMTVTVQHSAAGAIPVLGVPVQLSATPGRITGAAPLLGADTQAVLAELRDGI"
     gene            complement(12290..12670)
                     /locus_tag="VGE92_00630"
     CDS             complement(12290..12670)
                     /locus_tag="VGE92_00630"
                     /inference="COORDINATES: protein motif:HMM:NF025903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEY1312346.1"
                     /translation="MPLVRIDLQIGKPAAYRERIGQIVYRAMRETLNVPENDRFQVIT
                     EHAAPGMQFDRTYLGIERSDECVFVQVTLNAGRTLDAKRSFYKALADGLQQDLRLRRE
                     DVLISLVEVQKENWSFGNGEAQYA"
     gene            12799..13626
                     /locus_tag="VGE92_00635"
     CDS             12799..13626
                     /locus_tag="VGE92_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM sorting domain-containing protein"
                     /protein_id="HEY1312347.1"
                     /translation="MSNRHEVTSWLSRFAAMSLILAAAAGSARLARADTVVDAAFGDS
                     TFGTIDLTTGAVTPLGKSGVTLAGLALQSSTLYAAGFLGGVDSLYSVNPANGALSAIG
                     STGVSINDFGATSSGLYALDGNGNLYSVSATTGAATLVGSTGLNINQYWDSLSTNSST
                     LYFNNGATLYTLNTTTGKATAIGTDAGGAQMGALLFLNGVLYGGQNPLPGAAVTTGSV
                     DTLNTATGAATIVPGSKPVAGSFFGLAPIPVPLPAAAWLMISALGALGTLARRREAP"
     gene            13718..15397
                     /locus_tag="VGE92_00640"
     CDS             13718..15397
                     /locus_tag="VGE92_00640"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEY1312348.1"
                     /translation="MRAFAPAIFLGPALAFMSAVWAAPDPATELGLPAKVAASLHGID
                     AEHIRAHVRFLADDLLEGRGTGARGGDIAANYIATQFALDGLKPAGDQGGYLQNVRFT
                     GVHTLPASSASLQPAQGKALDLKLADDYVVANQTQTEKADIDAPIVFVGYGIEAPEYH
                     WNDFAGVDVKGKVVLVIVNEPPSKDPKFFNGEALTYYGRWMYKFEEAARKGALGALII
                     HRTDLASYGWNVVRSSWSNEQVYLADDKDPKLKAAAWIQLDVARQLFGYSNLKLDEMI
                     ASAGTPGFKARELPVRFKAHIVSEIRKFQSANVLGLLPGTDAGATQAVVYSAHYDHLG
                     IDPAMSGDNIYNGAVDNGTGVGMLLELAHAFATSQARPPHPVLFAAVTAEEKGLLGSN
                     YLGKHLPIPAARIALGLNFDAIPPIGVPESISVTGAERTSFYPIVEKTAKAFGFDIQP
                     DAEPGAGHYYRSDHFSLARAGVPAFSINTGVKFAGHTAEWGKAQRDDYTAKHYHNPSD
                     EYSPSMDFSSNAALAKFGFALGWEALLAKGTVNWLPGDEFEATRLTSGVAP"
     gene            15589..15753
                     /locus_tag="VGE92_00645"
     CDS             15589..15753
                     /locus_tag="VGE92_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312349.1"
                     /translation="MADNSSTGTFRMDLPKDLVSAAQRVPQPPGTEFKVEGWHNSWLA
                     RISEMLVGKD"
     gene            15858..16097
                     /locus_tag="VGE92_00650"
     CDS             15858..16097
                     /locus_tag="VGE92_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312350.1"
                     /translation="MRLARPVYESLPYLYIVIGGLSIFLFYLDPIGPRAVIAFLIGVT
                     VEIAALTLLLRRKDYRALSREYTGESIDFPSTLHG"
     gene            complement(16102..17439)
                     /locus_tag="VGE92_00655"
     CDS             complement(16102..17439)
                     /locus_tag="VGE92_00655"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY1312351.1"
                     /translation="MTMYLEQFKLHELPFRLSPDPQFLYLSKQHARAKAYMESTIWFT
                     DGFVVITGEIGAGKTTLIETFLRELQTDAVVAQINQTQLSPTAFLQTVLVQFGFSPFN
                     MKKPEVLATLNQFLVEQHIKGRKVLLIVDEAQNLSSRVLEEVRMLSGIETTKEKALRI
                     ILAGQPELNDKLNSSELVQLAQRVRLRFHLAALTKAETIAYIDHRLEVAGSQGRRIFE
                     EGTYGTIYKYTGGVPRLINTLCDTCLMAAYGRARDTVSKQDLEAAIEELRWVPFGKGA
                     RGAPTPEPEPAPEPEPESEPEVESEPQAEAPAKSEVQAAAPAAPEPVPAPSVEVAPTV
                     GRILLASAGKTVLERELKPGRLVIGRTPENDLQIDSKFVSRHHCQIVTQADSCVIEDL
                     NSTNGIFVQSKRVRQYNLNDGDVVQIGQHEIMYIDERSPRLRGEPDDEEDEEP"
     gene            complement(17459..18553)
                     /locus_tag="VGE92_00660"
     CDS             complement(17459..18553)
                     /locus_tag="VGE92_00660"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEY1312352.1"
                     /translation="MRLSRVGIVGAGQLGRMLALAGYPLGMHCLFLDRSAKAPAAQVA
                     PILTAELEDPTQLATLAGCSDVLTFDWENISAGALAPLEKLTKVRPPRAALEVSQDRL
                     SEKALFEKLRIPVAAHAAVDSELELARAVKKLGAGVLKTRRLGYDGKGQFILREASQI
                     GEAWAAIGGRDLIYEKFQDFSREVSIIGARSATGRMVFYPLAANSHGGGILHYNVAPF
                     ANARLERSARTYLKRLMNALDYVGVLTVEFFVVKGRLVANEMAPRVHNSGHWTIEGCV
                     TSQFENHLRAICDLPLGSTRPLGHAAMINFLGEMPDRERLLAIDGLAFHDYGKEPRPG
                     RKLGHCTILKRRAAERNSALAAALESIQWT"
     gene            complement(18550..19041)
                     /gene="purE"
                     /locus_tag="VGE92_00665"
     CDS             complement(18550..19041)
                     /gene="purE"
                     /locus_tag="VGE92_00665"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017341565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEY1312353.1"
                     /translation="MKALVGIIMGSSSDWETMQGAAAILDSLAIAYEVRVVSAHRTPD
                     LLFEYAASARERGLRAVIAGAGGAAHLPGMTAAKTSVPVLGVPVQSKGLNGLDSLLSI
                     AQMPSGVPVATFAIGAAGAKNAALFAAAILANENTEIRAALDAFRQAQTAAVLAKPDP
                     RSP"
     gene            complement(19062..19592)
                     /gene="yjgA"
                     /locus_tag="VGE92_00670"
     CDS             complement(19062..19592)
                     /gene="yjgA"
                     /locus_tag="VGE92_00670"
                     /inference="COORDINATES: protein motif:HMM:NF016626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HEY1312354.1"
                     /translation="MTSDTEGGTFDDESGESELERRSKSARKREAASLQELGVKLSAL
                     PDQEIKALDLPDNLFVALRDLRRLPSHGAQVRQRQYIGKLMRNIDPEPVLAKLAERKQ
                     RHDLEIRHFQTLERWRDRLLSEPVAGLDELLQEYPQADRSALTKLLEKAERERVEQRS
                     PVGARELFAFLRQLIG"
     gene            19665..21014
                     /gene="pmbA"
                     /locus_tag="VGE92_00675"
     CDS             19665..21014
                     /gene="pmbA"
                     /locus_tag="VGE92_00675"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF008268.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEY1312355.1"
                     /translation="MAVSSNPVRLAQGDLESIIERALEEARARGASQAEAAVSQDTGL
                     SVGVRLGEVETLEHQRDRSMGITVYFGQRKGSASTADFSLEAVRATVAKACSIARFTA
                     EDACSGLADAALMARAPADLDLAHPWNVSADRAIEIARTCEAAALGFDARINNSEGAS
                     LATHQGLHVYGNTHGFVGGYPTTSHSLSCVVLAGVGEDMQRDYWYSTSRDWRDLEPAE
                     QIGRESARRTIARLNPRKLSTRRAPVIFVPEIARGLIGHFVGAIRGSSQYRQSSFLLN
                     SAGEQVFPRGFSIAERPHIRKAMGSAPFDDEGVATHDRELIADGVLTGYILSSYSARK
                     LGLKTTGNAGGAHNLLVAPNLAGGMAAMLSQLGTGLLVTELMGQGVNGVTGDYSRGAA
                     GFWVENGSLQYPVAEITIAGNLRDMLKNIAAVGDDVDARGGTRVGSILLQEMTIAGE"
     gene            complement(21028..22332)
                     /locus_tag="VGE92_00680"
     CDS             complement(21028..22332)
                     /locus_tag="VGE92_00680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEY1312356.1"
                     /translation="MLTWLERLEDLLFQKSRSIGPPWGAPLRWLRYPAALLRDWLGGE
                     INIRAMSLAYTTLLSLVPLMVFSFAILKGIGARGDLKFLLHEFFRPMGSAADELTDSV
                     MQFVTNMRGELLGSIGLASLVYTVIATIQKIEGSFNFVWRVERPRSIARRFSEYLSVM
                     IVGPILLAVAIGLLGSAEHSPFAQWLQAVAPIAALLGAFAKVVPYIIVAAVFTFMYSY
                     IPNTHVQFRAAVIGGVTAGIIWALVGKVFSAVLVYSSQLVAVYTGFAIVLTTLIWVYL
                     SWLILLIGAQLAFYVQFPQYLRHGQEAIELTASDREQVALSIMYLIGRDFEAGRGYWN
                     TGRLAAELDVPGIALAPVLARLERGGLIVATEKEQFVPGRDPAGILLADILHAVRTLQ
                     ISRLTVELHRVGNAAQVMAEVEGAMRERLGSQSLKDLIAPQA"
     gene            complement(22342..23241)
                     /locus_tag="VGE92_00685"
     CDS             complement(22342..23241)
                     /locus_tag="VGE92_00685"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY1312357.1"
                     /translation="MQQVSHSAPLFESRLRGLKKIHQGKVRDIYDVDAQHLLIVTSDR
                     LSAFDVVLPDPIPFKGEVLTQISLFWFAKTQHMVANHLCALKVEDVVADAAERAQLAG
                     RAMVVKKLKALPIEAVVRGYLIGSGYKDYKLHGAVCGIELPAGLHMADRLPEPLFTPA
                     FKAASGQHDENIDFEAMVKLIGREYAAQVRSIALDIYRFAAEHARARRIIIADTKFEF
                     GVDAAGRLMLIDEVLTPDSSRFWPTQSYKPGISPPSFDKQFVRDYLESLHWNKKPPAP
                     HLPPDVVKRTSDNYREALKLLTA"
     gene            23325..23957
                     /locus_tag="VGE92_00690"
     CDS             23325..23957
                     /locus_tag="VGE92_00690"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEY1312358.1"
                     /translation="MSWKGMFFGAVVGFLLSRQVWGAVIGAVIGHLFDQSLNLGAAGA
                     GAGRGQSSLSIADEFFRTTFELMGHVAKSDGRVTEAEIDAARRLMHELKLGPREIGIA
                     IECFRSGKSTAYDAELSVERLREACGQRYDLLRAFMELQLRAALAGNGMSPPARAILT
                     RVAERLGMSGVEFAFMEAALRAPAAAAPPAGRLPRAMRNWKSTPLRLIKK"
     gene            23954..24109
                     /locus_tag="VGE92_00695"
     CDS             23954..24109
                     /locus_tag="VGE92_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312359.1"
                     /translation="MIKAYRRQMSRHHPDKLVANGLPESMAQVAKEKTQRIQEAYEEI
                     RAARGMR"
     gene            24120..24797
                     /gene="rpe"
                     /locus_tag="VGE92_00700"
     CDS             24120..24797
                     /gene="rpe"
                     /locus_tag="VGE92_00700"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005803358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY1312360.1"
                     /translation="MTSIISPSILSADFARLGEEVRAVLAAGADWVHFDVMDNHYVPN
                     LTIGPLVCEALRKAGIKAPIDVHLMISPVDALVPDFAKAGASYISFHPEATRHVDRTI
                     QLIRDSGCKPGLVFNPATPLNWLDYVIEKIDLVLIMSVNPGFGGQKFIPQALHKLAEA
                     RARIKASGRDIRLEIDGGVKVDNAAEIARAGADTFVSGSAIFGSSDYAATIKAMRAQI
                     ACGATEA"
     gene            complement(24945..25580)
                     /gene="crp"
                     /locus_tag="VGE92_00705"
                     /pseudo
     CDS             complement(24945..25580)
                     /gene="crp"
                     /locus_tag="VGE92_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016506431.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
     gene            complement(25678..26316)
                     /gene="coq7"
                     /locus_tag="VGE92_00710"
     CDS             complement(25678..26316)
                     /gene="coq7"
                     /locus_tag="VGE92_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017476331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HEY1312361.1"
                     /translation="MSTRSFSLIDRVLGEIDKAIKVLGAPARSLRPVPAAQQEASLPA
                     AQRAESARLMRVNHAGEVAAQALYQGQALTAGDPIVAAALHHAAAEEADHLAWCELRL
                     GELNGRTSLLNPLWYVGSFAIGAVAGAFGRGASLGFVAETERQVEAHLRGHMLRLGQA
                     DPRSLAILEQMTHDEIQHGVQASSLGGKQPPFALKVAMQLTSRLMTHGSYWL"
     gene            26429..26857
                     /gene="rplM"
                     /locus_tag="VGE92_00715"
     CDS             26429..26857
                     /gene="rplM"
                     /locus_tag="VGE92_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY1312362.1"
                     /translation="MYTVFSKPEETRGDWFLVDAAGKTLGRLASEIAHRLKGKHKPNY
                     APHQDLGDHIVVINAGTVRVTGAKLTDKFYHQHTGYVGNLKSISLGKLLVEHPERAVE
                     FAVKGMLPKGPLGRRMFRKLHVFGGKDHTHQAQQPKALEI"
     gene            26874..27278
                     /gene="rpsI"
                     /locus_tag="VGE92_00720"
     CDS             26874..27278
                     /gene="rpsI"
                     /locus_tag="VGE92_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY1312363.1"
                     /translation="MASKTAVNSFYGTGRRKTSSARVYLRQGTGRVSINGRSLDEFFG
                     RETGRMIVQQPFGAVQLGGKFDVDAHVEGGGITGQAGAIRHGITRALMAYDESLRAPL
                     RKAGFVTRDAREVERKKVGRRKARRGTQFSKR"
     gene            27304..27377
                     /locus_tag="VGE92_00725"
     tRNA            27304..27377
                     /locus_tag="VGE92_00725"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27336..27338,aa:Gln,seq:ctg)
     gene            27427..29301
                     /locus_tag="VGE92_00730"
     CDS             27427..29301
                     /locus_tag="VGE92_00730"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEY1312364.1"
                     /translation="MSGERNSSGRLAPLALAALGVVFGDIGTSPLYAMSSCFTGPNIA
                     VTPAHVLGVLSLIFWSLVLVILLKYVSVVLNADNKGEGGVLALTALVINTERESPRRK
                     LYGTLGILGASLFFADGAITPAISVLSAIEGLHVAAPDAQIPVLPITAIVLVGLFLIQ
                     RHGTGTVGKFFGPVMLFWFAIIAAMGVSWIVREPKVILALDPTRALALLAQHQVSSLA
                     VLAGVFLTVTGGEALYADMGHFGKAPIRLGWICIVMPALVLNYFGQGAMLIEDPKSIS
                     NPFYLMAPAWSLWPLVLLATAATVIASQAVISGVFSVTTQAVSLGLLPRLKVEHSSAD
                     NAGQIYVPTMNWILMIAAIALVMGFGSSAALGGAYGLAVSGAMTIVTPLTLSLIKSRT
                     GKDSRWLRTGLSLLFIVDIAFLLANLTKLEDGGWLPLSSGLIIYWLMQTWQRGRASVM
                     ARLLREQKPVRDFLEDLKRAPPVRVPGTAIFLDQKASGIPRALLNNLKFNRVMHERVV
                     LLTVVTREQPRIATSKRLTVTPVCEGIVRIVAQVGFMEKPNISEILREADAHGVPYDP
                     ECTTYFLSREELLAGQRSDLPALRRRAFMQMARSAQVIADYYGLPPNRVIEIGTRMEI
                     "
     gene            complement(29298..29966)
                     /gene="rpiA"
                     /locus_tag="VGE92_00735"
     CDS             complement(29298..29966)
                     /gene="rpiA"
                     /locus_tag="VGE92_00735"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004925711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEY1312365.1"
                     /translation="MSSLENKRRAAEAALAYVQPGMVLGIGTGSTVTQLIAALKQRKI
                     QLSGAVSSSRQTSELLRTSGILELDLNSAGPLSLYIDGADEATADGVLIKGGGAALTR
                     EKVLAAAAATFVCIIDDSKLVEVLGKFPLPIEVIPMARSYVARQLVKMGGQPVYREGV
                     ITDNGNVILDVHNLELDEPQSFESAANQIAGVVTVGLFALRRADLVIVAGDAGVKTLD
                     CRPA"
     gene            complement(30210..30674)
                     /locus_tag="VGE92_00740"
     CDS             complement(30210..30674)
                     /locus_tag="VGE92_00740"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEY1312366.1"
                     /translation="MNYWLLKSEPDSFSIDALSKAPKQTTAWDGVRNYQARNMLRDSM
                     KQGDLAFFYHSSCEVPGIAGIVSIAREGYPDKTAFDPKHHHYDADSNTDAPRWFVVDV
                     KLVRKLKRIITLDELKQHAAKSLKNFVLLRRGNRLSVMPVAAKDWEFVLTLE"
     gene            complement(30671..31258)
                     /locus_tag="VGE92_00745"
     CDS             complement(30671..31258)
                     /locus_tag="VGE92_00745"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEY1312367.1"
                     /translation="MKPTLRKHLRNQRRSLNTAVHAQRSNLAAKAIAGLPMFRAGKRI
                     AVYLPFDRETDTAALIAAARRRGVGIWVPVIVDRRRSRIRFYPLSGKLQRGTYGIAVP
                     SMRAQPLPPRWLNLIVLPLVGVDAQGRRLGMGGGFYDRALEFRRHRRSWAGPRLVGLA
                     FDCQRTATSFAQEWDVKLDSLATESGLHHYARGAS"
     gene            complement(31277..31462)
                     /gene="ssrS"
                     /locus_tag="VGE92_00750"
     ncRNA           complement(31277..31462)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VGE92_00750"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(31503..31817)
                     /locus_tag="VGE92_00755"
     CDS             complement(31503..31817)
                     /locus_tag="VGE92_00755"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEY1312368.1"
                     /translation="MSDNQARVSVRILEKEYHITCPIEERSDLLDSAEFLNLKMREIR
                     DSGKVVGLDRIAVIAALNMANELIRFRNRDGDLESDVGGRLRILRERVESALEKGQQL
                     EL"
     gene            complement(31814..>32017)
                     /locus_tag="VGE92_00760"
     CDS             complement(31814..>32017)
                     /locus_tag="VGE92_00760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02449 family protein"
                     /protein_id="HEY1312369.1"
                     /translation="VELELKRLEKRLEDLILTVSQIKEENRALRQRQETLTAERASLL
                     QKNEQVRARVEAMIGRLKSMEQA"
BASE COUNT         5952 a         9780 c        10158 g         6127 t
ORIGIN      
        1 gtcatgtggc cggcaaagtc gctcaggtag ctaaaggcgc cgtgcggccc gaacatcaga
       61 tcgacgaact gagtcgtgga ggggaagcgc gcattgccgt ggccacccgg gctgccggca
      121 ggaccgaata tcgcgaactc gatgtcgctg cgctcggaaa cgatgcgcgc cttgatgagg
      181 cccgcgagct ctgcggcatg atcgacatgc agatgcgtga gcagcacgat atcgaggcga
      241 ctcaaatccg cgcccgcctc gccggcgcgc acaaaggtgc cgggtcccgc atccacgaga
      301 atgcgcggcg taccgtcgag catgaccagg tagccggcgc cggcgcggcc ggtcgcgccg
      361 gggccgccgg agccgagcac cagcaaggac agcccgcggg acgcctcatc ggcggccgcg
      421 ccgcgcgcgg caagatcaag cgctccgagc agcagcaata gcgcgcagag attgatccgt
      481 gagcgatggc acatcagcga gcagcctcga gcgaatcggc gagcagctcc gccacgcgca
      541 ggccccacgc gtagatggtg agcgagggat tgacgcggct cgagcggggt aggacgctgc
      601 cgtcgaccac gtacagtccc tcgatgccga ataccgcacc attcgcagcg acgaccgagg
      661 tgttcgggtc cgtgccggcg atcaatgagc cgtcgatgtg cgccgtgccg tccagaccga
      721 tgcgttgcgt caagctgatg aacccgatgc gcagcagtgc gcgcttgaac gcgcgcgtga
      781 acgagcggtg ttcggcttgc gaggcgggcg tgcgcagcgc atcgtagtcc atgacccgtt
      841 ccggcgggtc gccgcgctcg aggactcgat tgtcaggatg cgatccgtcc tcggtttgca
      901 ggaagaatcc ataactatgc cgcccgatga ttcgtgccac caagcgcggc acgaaccttg
      961 gaatcagcgt tgcgatcatt tcggcgtcaa accccaaggg ctgcacgctg ctgtgcgggt
     1021 gttccgtgtg ggtggtgatc atggtcttgc gcaacaggtc cgactgcggc gagacagaca
     1081 ccgccacgag cgccgtcaag acgtgcttct tgaggttgcg tcctaccgcg ccgagcgtcg
     1141 ggaaatcgga ccgaccggcg tgcgcggcga ggtagcgcag gaggagccga ggcgaatgca
     1201 gggcgccggc ggccagcaga accgccggcg cccgcagttc ctcgccgttc atgagcttca
     1261 cgccgccgat gcgcgcagcg tccgagcgag ccggcaatag ctcggccact tcggccgagg
     1321 tgcgtatcgt aagattgggt ctgtcggcca ccggccgcaa gaaattctct tccgcgtcgc
     1381 ccttgagatt ggcggcgctc gcgaacccgt cgaagtgggt cgcttcctcg ggatggcgca
     1441 gaatatcggc ggacaaggcg agcggcaggg gcgttgactg ccaggagcgg tcggacgcgc
     1501 ctaagcgctc gagaatcagc ttgagccccg gttcgcagga gaatgtgcgc accgccagca
     1561 gccgctcggc ctcatcgtag tagggcgcga attcggccgc gccaatcggc cagccgcggg
     1621 ccgagtacgc ggggtccgcg gcaaactcgg atcccgaata gcgcaacacc gccgccccgt
     1681 accacttggt cttgccgccg agattgaaat gttcctgcgg acaaatcggc cggccggtgc
     1741 catcgatcca cggctccttg ctcaagaact cgccgtgcgt caccacccgc cgatcgtcca
     1801 aggtggatcc gtcgtgcggc agaggggcac ccttttccag cagcaacacc ttgacgccgc
     1861 gcaatgccaa gcgataggcg gccgcggctc cgcccgcgcc ggaaccgaca atgatcacgt
     1921 cgtaggaagg gaatgaagcc actgcgttcg ccgcgccgat gattgcggta atgtcgcata
     1981 gggaccagcc gcggacaacg tgaagcgcgc gaatcgattt tctgcgagcc caaggcggat
     2041 gcggcgagaa ctcgccgcat ccgcgccggg gcttacccta gcgattccac gtatcgcgaa
     2101 aatcgaatag ctcgaacagg tcggagatgg ccggcgagtt caccggcaca tagggattgt
     2161 gacgcgcggc gaccggattc gggaagttgt cgcggctcct atgcgtcacc ggatgcatgg
     2221 accagtttct ctcgatgaat ttgaggatcg aaacgtgatc cgaatagtcg tgcgagatgt
     2281 gccccggttt ggtgtagcga gacaccacga gcagcggaat gcgcgtgccg tcgccgaaaa
     2341 agtcgaccgg ttgcacgtaa ccggagtcgt agtagccgcc gccctcatcg aaggtgatga
     2401 aaatggcggt gtccttccac agctcaggat tcgccttgac cgcatcgacg atcttcttcg
     2461 agaacccttc gaatagatcc agcttcgagg agctcgggtg accgtcgacc agaccgctcg
     2521 gcttcacgat ggagacggcc ggaagcgttc ccttggcgat gtccgagtac aaatccgcgg
     2581 tgtcctggat gtgcgccgcc cgaatcttcg gattcgccat gatggaggtg tcgtactgga
     2641 atggattgca gatgttgcag tattcgtcgg tattcgcacc catcgcccca tagttcagct
     2701 ggtacgggtc gggaacatag ttgttccatt gatcgccgta gtatttccag gagacaccct
     2761 tctccagaag ggaatcgccg atgctggggg tggaggaggg cggaatggtg aacaccgtgt
     2821 tgttggcgtt ggtgtcggtg aaggcattgt tgccgttgcc gaaatagccc gggttgtagt
     2881 tgttgagcag atagtaatgc ccggcctcgc aacgcggatc gatcttgcga ggcagcgatt
     2941 gcaggtactt gacgacttcg gcgacgccgg gcgcttggct attcgcgcat tcgctgtagg
     3001 agccgccgcc gtacgaggcc gaaccgaacg aaccgccgcc atagccatct tcgctgtacc
     3061 aattgttggt gccgggcgcg ggattcgggt tctcgacctc atcgacgaca ccggcattcg
     3121 cggtgccctt ggccacctcg acatcgtgcg gcggcggcag tgcgtggcct gccccgtcgc
     3181 tgaaccaaat ggcgtcgccg tgcccgaaca tgatgtgatt ggcgccggtg ccgccattga
     3241 ccgactgatg gaagttgtcg ctcatggcgt aatcatccgc caggctcttg aaataaggcg
     3301 catcgccctg ttgcacgttg taaaagccga gcgctgtcga gccctcgccc gtggttttgg
     3361 cgccggccga atactcggtg ctgaagttcg ccggctgcgg caagccgttg gtgcccgcgc
     3421 ccaccgtgac ttcaacccag gagaacaatt tcgagtcgca accggacgga atctccggcg
     3481 tggcatgttc cacgctgcaa tcgagctgct gccacatctg gtagaagcga tgcacgggac
     3541 ttgccgcata ggcgttgtag tcgaacgtcc tgccgttcgt caattgaaac gggcctgcgg
     3601 gtaatgcgct gacgtgtgcg atgcgttggt ccggggtatg cgaggtttga ccggtgccgc
     3661 cgctgatcag gtactgatag tagtcctcgg gtagaccgtt ctccgactgc tgcgcaagcg
     3721 cgaggttgtc gccggtgaca tacgaatcct tggggccgcc caccaatggc gccgggagca
     3781 caccgttcgg aaacgtcgtc ttggccggat tcagcaagaa ggcatcggcg gcgggatccg
     3841 cggcggcgtg ctgttgcgcc ttgtagaaat tcggacccgg agagccgtcc tgcttgatga
     3901 tcccttccga cagcaggttc cacacatttt cgccgttctt gggctcgtag gtggcgaaca
     3961 catggtcgaa gctgcgattc tcgccgatga tgacgatgac gtgcttgata ggcgtacgtg
     4021 tctgcgcgct gtggtcggcc accacggccg cggcgcttgc cagcggcgcg ccgagtgcca
     4081 gctgcaacac cgccaaactg ctgaggccca cacgcaaccc gttaagactg cgcgacgtgt
     4141 aattgctgaa tggcatacga atcccctcta tttggtttgc tgaccggcgg cagcaaactt
     4201 tttacgggct gcgtatttca acgatgtgat cctattgtga agcctgcatc aaatcgcatt
     4261 gtagatttta ccgcgctgcg gtggcatgcg ccgccgcttc gactcggatg agtcccatca
     4321 tgccaccatc ttcgtgttcg agcagatggc agtgatacac gaaagtaccg atcgtgttcg
     4381 gatcgcgaaa atccatgcgc agccgcacgc tcggatagcg cagcatccgg ccgttgaaat
     4441 acggcacgtt caccgtgtcg cgcaagaacg gctcattgac gggcgtgccg gaccaatcga
     4501 gcagcagaaa atggatctga tgaatatgaa aggcgtgcag ttcggcggag cggttctcga
     4561 tgatccagtc ctcgacgtcg ccctgatgcg cgacgatgtt ggggatgtcg gattgcgggt
     4621 cgaacgccag cggcgtttgg ccgtccaccg tcaggtagaa ctcggtggcg ctgttcggat
     4681 cgtgggcatc gctgagtttc tctgaaaaat acaatttgcg cacgcgcacc ggggcgacct
     4741 tgcccaacca gggcagtccg gccggcggca gcgccttggg tgccgctgac agccgcgcgg
     4801 acggttccgg cgcatcggac gctgcgatca ccgcggcgag cgcgcgattc gggtcgttct
     4861 cgccgccctg tccggtgtcg acggttcgcg tcacgagcag agccggcacg ccggcgggcg
     4921 gcccggtcac gataaattcc acgcgcgcgc ccggcggcac gccgatgtgg tcggtccatt
     4981 ggatcgcatt ggccggattg ccgttttggt tgagcggtac gccgtcgatc gccacgatgc
     5041 cgagctgttg cgaagcgcga ttgaagagca gcgccaagtt caaatacgta atggcggagg
     5101 cgttcagcac gcgccagagt tggcgttccc ccggccgcat ctgcagagac gccggcggat
     5161 agtcggggta gggaacggga acgaaattta ccgacaggtc cttcgccggc ttgccgaagc
     5221 ccgtgccgtt gttggccgaa tcgccatcgc ggtcgatgaa catcttcgga accacgggtt
     5281 cggactgcga cggcggcgcg tccgggttca acaagtcctg atcgcggacg atgagtacgc
     5341 gctcgggcag gcccgcgagc tcgggattcg cgcgctcgat gccctcgacg atgagcgcgc
     5401 cggatgcccc gcccaacacc tgcgccttgc tgaatccgtg aatgtgcggg tggtaccaat
     5461 acagacccgg cggctcgctc gcgggcacgc gaatccgata ttcgaagggc ggatcgccgg
     5521 gctggatgga ggtcttcagc acctcatcct ggtggcagac gggaggcacg gtcatgccat
     5581 gaaagtgcag gttggtcgcg gtcagggaca tgcgagcgct gctgcaggga ttctcggacc
     5641 gggcggcgac gccatgctgg tgcgccgggg acgcagcgcc gtcgaggtcg gtcagtgcgt
     5701 tcttcagatg aaggaccaac aggtcgcccg gatttacgcg caaggtcggc gcttgcgagc
     5761 catcgccgag cacgtagcaa tagcgaaccg aaccgtcgct ttggcgcgcg ttgtgcacgg
     5821 tcaagtcgag cgagagcacg ccgttttgac tgcgcagatc cttcggctcc gggatcacgc
     5881 tgccgggcgg tggccgcgga cacacctcct ttgggtcggc gccggccgcg cgcgcggcac
     5941 agggcagggc cgcgcatgcc gccagcatca gccaccctaa gcgcgctcga ttcgccacac
     6001 cggctactgt atgcggccta gacgaagttt tcatgtcagc acctggtcga ggcgcacgac
     6061 tagttggcgg cgccgaattt cttggtgatg gtgagtcgcg ccatgatcgg ctcgagcgga
     6121 tgctcatgca tatcggcgcg cccatccggg tacgtgccga tctcgttttg cagccgatcg
     6181 acgtaccaaa attcggccgc ggccgcgtgg ctattgagga tattgtagat ccctaacgag
     6241 gcgctccagt tcgacggcaa tgcgtaggct gcgtccaggt tgagctgcgc gaagcctttg
     6301 ccgttcacct gacccgcggc cgtcggtgcg ttcgcgcacg aagtggccac accggggaaa
     6361 tcgtgcacgg ccgcggagtc gacgcagggg ccggacgaga gagcgtaatc accgagatag
     6421 cgcagctcca ggccgccgct ccagggtccg aggttggtca agtacagcgc gagcgaaccg
     6481 ctcgccacag gcgcgtccgt aatgtatctg cccaaatgac ccgtgccgtc atcgaagtcg
     6541 cgcgtgaatc gggtgtgatc gcctgaatag ctgccataga attccagcca ctgattgatc
     6601 ttgtaagtca gattgacttc atagccatag cggcggctcg gtggcccagc cgaatctgcg
     6661 ccgacatccg gatcgatgat ggtctcggat tgctgccata aattgtagac cgccaaggtg
     6721 aacgtcacat tgcgcgcagg cgctgcgcgc aggccaacct cttgcccttc ctgcttcgcc
     6781 aacagcgagg tgcgcggaag tcccaagtcc acgctcgtgt cctgattgac gccgcgaata
     6841 tcggcgctat gaaatcctcg gccggcgctc aagtagaact cgagcgcatc gttcggcgtg
     6901 tagatcaaac tgcccttcgg ttgcgccagc ccctgatgga cggtgccggc attcgaatag
     6961 cccacggtct cgtgcaatgc ggccaggtaa tcgatgtccg tgccatgttg gtagtcctcg
     7021 cgaaaaccga gcacgctgcg aaacttcgac gtccagcggg tcgtcgcctg cacaaacgcc
     7081 gcgccggcaa aaaggtacac ctgatcgaga tcgctgaacg agggaggggc cgtgctcgcc
     7141 ggcaggtccg acatcgcgag gctgggtgcg cgcccgaccc gcagggcgtc gtaacgcgtg
     7201 aggcctccga cgagcagctc gctcggtgcc aagaacgacg tcagcgcaaa gcgatagctc
     7261 agcgcgcctc cgcccgcatc acgccgctcg aactggtctt cctgatcgcc gtagagggga
     7321 tcgcgcaggt agtgggtaaa gtcattgtag agattcagat cattccggat gtagaacgtg
     7381 ctggccgtca gctggccgtt gccgatggcg tcgtggaact ccgccgcaac gctcgcacgc
     7441 tgcgcgcggc caccgtccgt cgggtcgaga gttccgaagc gatcgggcac cagaccctcc
     7501 gcgatggtcc ggatcgggat gtcggtggtg ttggtccaga gctggtggta gtacatcccg
     7561 gtcagcgaat agccgttcgt ctgatcgcct tgactgtagc gcagcacgaa gttgcctttg
     7621 cgggcgtcgt ccggattgtc gaacgggccg tcgtagtgct ggccctcgat cccagccagc
     7681 aggtggccgt caccgagcgg cgtcgaaccc gcggcgaaaa cacgctgaaa accgtaagtt
     7741 ccgagcgtcg cggatacctg atcgggaatg gtgtcgcgat aggaaattcg caccgaaccc
     7801 accgcgccga aatccccgac attcgcgtaa tacggtccct tggtgtaggc gatcccatcg
     7861 gcaagctccg ggatcatgaa attcatgtcc gtatacccct gcccatgggc atgcgtcggc
     7921 tggttcacgg gcatcgagtc cacataggtt tcgaggtcgg tgccgtgatc gaggttgtag
     7981 ccgcgcagca gatactggtt ggccttgccc tcgccgctat gcagagtcgc gatgagtccc
     8041 ggaacggtct ccagcaattg acccggccga tacgccggcg tcagctgaat ttcctcgtcc
     8101 gtgacgatac cttcgctggc actgcctgcg gtccctaaca gccccaggcg ttgcgcggta
     8161 accgtgattt cattgagggg gaaatcggta ctcgcggcca cgctcggcgc cggttcggcg
     8221 caggcaaccg cgaggcagag cgcgttgatc cctgccgctg cgcacaacag gccgctgcag
     8281 gccgcgcgag caggtcgtag gcagcgctgg tgcgtcatgg ccgatctcgt aagacgattt
     8341 acgtatttgt attcgaggaa agcccgccgg cggatgcgct gagggattcc aaaaaatcct
     8401 gttacgcgtg ccttgcagtg tgtctgaaag caggaaaaaa tcccaggttg cgacgccgta
     8461 tttcgatgaa atcgggcgac gcggcgcgcg cgcccggtga tttgttagac tcatgttcat
     8521 gaatcgagtg tcgccacgag tcaggccctt gcgttttcga acgctggcgt tgtcatccgc
     8581 gcggccgcct gccttccccg ccgccactgc gccctcccgc gcagtaaatg cgtaaccacg
     8641 ctgaccgcgg gcgtcgccgc ttcatccaga gtttgggtct gggcggcgcc gccgccgcgt
     8701 ttgggctgct gccctgcgct tcgcgcagcc gcgccgcgac gagcgcagcg ccgctgagcg
     8761 gcaccgaatt cgatttgacc ttgagcgaga ttcggttgaa cgttcggggc gaatcgcgcc
     8821 cggccaccgc ggtcaatggc ggcgtacccg gacccacctt gcactggcgc gaaggcacga
     8881 ccgtgacgct gcgtgtgcac aacgccctgc gcacgccgag ttcgatccat tggcacggaa
     8941 tcgtcttgcc cttcgaaatg gacggcgtgc ccgggatcag cttcgaaggg atcgcgcccg
     9001 gcgagacctt tacctatcgc ttcccggtac gccaaagcgg cacgtactgg tatcacagtc
     9061 acacgcgctt tcagcagcag acgggtctgt atggcgcgat cgtgatcgaa ccggcgggcg
     9121 gtgaacccat ccgcgcgcac cgcgatcacg tgctgctgtt gagcgattgg acggacgaag
     9181 atccggaaca tgtctatacg gcactcaagc aaatgagcga cttttataat tttcagttgc
     9241 cgagcgttgc ggatttcgcc cgcgacagcc gttcgatggg cgttcgggag gcgctcgcca
     9301 agcgccgcat gtggaacgag atgcgtatga atcccaccga tctcggcgat gtatcgggcg
     9361 cgacgtatac gtatctcgtc aacggcgctt ctgcggcggc aaattggacc gcattggcca
     9421 tgcccggcga gcgcgtgcgg ctgcggttga tcaacggatc ctcttccaca ttcttcgatg
     9481 tgcgcattcc gggactgcca atgaccgtca tcagcgcgga tggccaggcg gtcgacccgg
     9541 tgacggtcga ggaaattcgc atcggtgcgg ccgagagcta cgatgtgctc atcgagccca
     9601 ccgaggatcg cgcctgtacg atttttgcgc agtccatgga tcgaaccggt ttcgcacgag
     9661 ccacgctcgc accgcgcgcg gggatggagg cggcggtgcc ggaactcgat ccgaagtcat
     9721 ggctcagcgc aggcgactta gggttgcgcg acatgtccgg gatgtccggg atgtcaatga
     9781 gcggcgcgcg cagttccaat ccgagcatcg acatgcgcgt gccgcagccc tcgcgggcgc
     9841 tcgacgaccc cggacctcgc ctgcgcggca acgggcggcg cgaattgacc tatgcggatc
     9901 tgcacaccat cggcggctcc cgggatccgc gagcggcgac gcgcgagatt cagctgcgct
     9961 tgacgggcaa catgcagcgc ttcatctggg gcttcgatgg caagaaattc tcggaagcgg
    10021 atgcggtgca tttcgatcgc ggcgagcggc tgcgcatcgt cttgagcaac gacaccataa
    10081 tggcgcaccc catccatctg catggaatgt ttggcgaact cgaggccgcc aatggcgatg
    10141 tgctggtacg caagcacacc ttcatcgtgc agccaggcaa acaactgagc tatctcgtca
    10201 ccgccgacaa tccgggtcag tgggcgtatc actgccacct gctgtaccac atgatggccg
    10261 gcatgtttcg ccaggtagtg gtggcatgag gcgagcgggg cgcgcggcgc tcggggcgtt
    10321 ctgctggttg gcatgttcgg gtgctgcgct cgccgacgtg ccggagggtg cgatcgcgca
    10381 tgcgcccgat gcggcgctgg agcgccccgc ggcgagccag caagtcacgc cgtcgatgat
    10441 gccgggcatg gacatggacg acgccacgaa tcagcatttt gtctggctcg agaatctcga
    10501 ggccgtggcg ggaaccgtgc acggtggtgc gtgggacgcg caggcgtggt ttggcggcga
    10561 tttgcacaag ctatggctga agagcgaagg cgactctctc gacggccgca accaacaagc
    10621 caaactcgag gcgctgtggg atcatgccgt cctgccgctt tgggatactc aactcgggtg
    10681 gcgccacgac tttggcccgg gaccttcgcg ggattgggcg gcgttcggcg tacaggggct
    10741 gacgccctat tggttcgatg ccgaaatcac cggctatgtg ggtgagcaag gtcgcacggc
    10801 ggcacggctc aagaccgagt acgatctgtt catcacccag cgcctggtct tgaagcccga
    10861 aattgagtta aatgcctacg gcaagccaga ccgggagcgc gcattgggcg ctggattggc
    10921 gcagggccaa ttcgaactgc gcctgcgcta cgcattcacg cgacgtgtgg cgccgtacct
    10981 ggggtacgtc tatgaggtta agtttgcgca gtccgcgagc ttggcgcgcg cagcgggtgc
    11041 gccggcgctc gagcatcgcg cggtcgccgg cgtcgcgttt cttttctgag tcgtcgagca
    11101 aaacatgaat tcaaaagctc cgccgttggc aggcatgacc gtcctcgatt tctcgcatgc
    11161 gctcgcgggt ccctacagca ccatgttgat ggcggtctac ggtgcgcggg tgatcaaaat
    11221 cgagagcccc gagggcggcg atatcgggcg cgcgtggggt ccgccgtttc aaggcgatga
    11281 cgccgcctat ttcgtcggga ttaacagcgg taaacagagc gtcgcgatcg atctgaagag
    11341 ctccgcgggt cgcgacatct gccgcaatct ggcgttgcag gcggacatcg tggtcgagaa
    11401 cttcaggccc ggcaccatgc agcgcttggg actgtgcttc gaatcgctgt cggcgatcaa
    11461 tccgcgtttg atttatgtgt cgatctcggg atatggacag acggggccgc gaacactcga
    11521 acccgcaatg gatctcatca tccagtctgc ctcgggactc atgagcatca ccgggacgac
    11581 ggcgggcgaa accgtgaaga ccgggcactc gatcgtcgat atcagcgccg ggctgttcgc
    11641 attgatcggc gcgttgctgg cgattgaagc gcgccatcgc accggcctgg gtcaactggt
    11701 ggatgtgtcg atgatggatg ccgtcatgtc gacgatgatc gcaaacttcg cgcgctacct
    11761 cgcatcgggc gtcatcccgg gcccgatggg gacgctcttc gagagcatcg tgccgtatcg
    11821 caactatcga tgtgccgacc gcgagatcac gatcgcggtg gccagcgata aactttggca
    11881 gcacttttgc gaggcgatcg ggcgggcgga tctgtcgact catccggact acaacgccaa
    11941 cgcattgcgc gtgcggaacc gggccacgct cgagccgatc ctggaagcaa tattccgatc
    12001 cgaatccgcg gcacactggg tggctcaatt gtcgaagcac ggtgttcccg caaccctcgt
    12061 gcgaaatctc aaggaagtca tcgacgacca acagactgcg gcgcgcgata tgaccgtaac
    12121 ggtgcagcat tccgcggcgg gtgcaatacc cgtgctgggc gttccggtgc agctgtccgc
    12181 tacgccaggc cggatcacgg gcgccgcgcc gctgttgggc gccgacactc aagcggtact
    12241 cgccgagctt cgcgacggaa tttaacggcg ccgccggagt tgcgccgtgc tacgcatatt
    12301 gcgcctcgcc gttgccgaag gaccaattct ccttctgcac ttcgacgagg ctgatcaata
    12361 cgtcttcgcg ccgcagccgc aagtcttgct gaagtccgtc cgccagcgcc ttataaaaac
    12421 tacgcttggc gtcgagcgtg cggccggcat tcaaagtgac ctgtacgaac acgcactcgt
    12481 cggagcgctc aatacccaag tacgtgcgat cgaattgcat gccgggagcg gcatgttcgg
    12541 tgatcacttg gaagcgatcg ttttccggca cattgagagt ctcgcgcatt gcgcgataca
    12601 cgatttgtcc gatccgttcg cgatacgcgg ccggtttgcc gatctgcaaa tctattctca
    12661 cgagtggcat ggcattctcc ttgcgttgta tgtcaaacta aagcagcagt tgactcgatg
    12721 caaacgttcg aggacccgta gaattcgcga agcgccggcc gtgtgccggc gtttcgcgtt
    12781 ttgatccagg agaatattat gtccaatcga cacgaagtca cgagttggct atcgcggttc
    12841 gctgcgatga gcctcattct cgcagcggct gccggatccg cgcggctcgc acgggcggac
    12901 accgtggtgg acgcggcgtt cggcgacagc accttcggca cgatcgatct gacgacgggg
    12961 gccgttaccc cgctgggcaa atctggggta actctcgccg gcttggcatt gcaaagctcg
    13021 acgctgtacg cggcgggttt tttgggtggt gtggattcgc tgtattcggt caatcctgcg
    13081 aacggtgcgc tgagcgcaat cggttcaacg ggtgtgtcga tcaacgattt cggcgcgacc
    13141 agcagcggct tgtatgccct cgatggcaac ggaaatctct actccgtcag tgcaactacc
    13201 ggcgctgcca cgctggtcgg gtcgacggga ttgaacatca atcagtactg ggactcgctg
    13261 tcgactaatt cgagcacgct gtacttcaac aacggggcaa ctctctatac cttgaatacg
    13321 accaccggca aggcgacggc gatcggcacc gacgccggcg gggcgcaaat gggcgcgctg
    13381 ctttttttga acggggtgtt gtacgggggt cagaacccgc ttccgggcgc ggccgtcaca
    13441 acgggctcag tcgacacctt gaacaccgcg acgggcgcag ccacgattgt gcccggctcg
    13501 aagccggtcg ccggctcatt cttcggtctc gcacccatcc ctgtgccctt gccggcggcc
    13561 gcctggctga tgatctcggc gctcggcgca ctgggcacct tggcacggcg acgcgaggcg
    13621 ccgtaaagtt cgcgacggac ccgtgaaacg ccggccatgc gccggcgttt tgcattttgg
    13681 gttagtctct gccgttactt ttcgttggga gcagaggatg agagcattcg caccggcaat
    13741 tttcctaggt cccgcgctcg cgttcatgtc ggcggtgtgg gcggcgccgg atcccgccac
    13801 cgagctcggt ctgccggcga aggtcgccgc cagcctgcat ggaatcgacg ccgagcacat
    13861 tcgcgcgcat gtgcgctttc tggcggacga tttgctcgag ggccgcggga cgggcgcgcg
    13921 cggcggcgat atcgcggcca actatattgc aacccagttc gcgctcgatg gacttaagcc
    13981 ggccggggac cagggcggct atctgcaaaa cgtccgcttc accggcgtgc atacgctgcc
    14041 ggcgagcagc gcctccctgc agccggcgca gggcaaggca ctcgatctga agcttgccga
    14101 cgattacgtg gtcgccaatc aaacccagac cgagaaggcc gatatcgacg cgcccatcgt
    14161 cttcgtgggc tatggcatcg aagcacccga ataccactgg aacgattttg cgggtgtcga
    14221 cgtcaagggc aaggtcgttc tggtgatcgt caatgagccg ccgtcgaagg atccgaagtt
    14281 cttcaatggg gaagccctga cctactacgg acgctggatg tataaattcg aggaggctgc
    14341 aaggaaaggc gcgctcggcg cgctcatcat ccatcgcacc gatcttgcca gctacggctg
    14401 gaacgtggtg cgcagttcct ggagcaacga acaggtttat ctggccgacg acaaggaccc
    14461 caagctcaaa gcggcggcgt ggatacagtt ggacgtggcg cggcagttgt tcggctactc
    14521 gaacctcaag ctggatgaga tgatcgcaag cgcgggcacg cctggattca aagcacgcga
    14581 attaccggtg cggttcaaag cgcacatcgt cagtgagatc cgcaaattcc agtccgcgaa
    14641 tgtgctcggc ttgttgccgg gcaccgatgc cggcgcaaca caagccgtgg tctattcggc
    14701 gcattacgat catctcggca tcgatcccgc catgagcggc gacaacatct acaacggcgc
    14761 tgtggacaac ggcaccggcg tcgggatgtt gctggagctg gcgcacgcct ttgcgacctc
    14821 gcaggcccgg ccgccgcatc cggtgttgtt tgccgcggtg accgccgagg aaaagggctt
    14881 gctcgggtcg aattacctcg gcaagcatct gccgatcccg gcagcgcgca tcgcgctcgg
    14941 tctgaatttc gatgcgatac cgccgatcgg tgttcccgag tcgatcagcg tgacgggtgc
    15001 ggaacgcacc tcgttctacc ccatcgtcga gaagacggcc aaagccttcg gcttcgacat
    15061 tcaaccggac gccgaacccg gcgcgggcca ctactaccgg tcggatcact tcagtctggc
    15121 gcgcgccggg gttccggcgt tttcgatcaa caccggcgtg aagtttgccg ggcatacggc
    15181 cgaatggggc aaggcgcaac gcgacgatta cacggcaaag cattaccaca acccaagcga
    15241 cgagtattcg ccgtccatgg atttctcgag caacgcggcg cttgccaaat tcggtttcgc
    15301 attgggttgg gaggcgttgc tcgcgaaggg cacggtgaac tggctgccgg gggatgagtt
    15361 cgaggcgacc cgcttaacta gcggcgtcgc accctagttc cgtgaacaaa ttcgcgttgc
    15421 gtcaataact taagcgacat aatccggcat tgtgcggccg cgtcgcacgg cggcgacggg
    15481 cataattcgg cgcgcgtcat gcaatgctca ataacgtgcg caccgtccca tcgatggggc
    15541 catgggactc gataccctcc gcaacggatc aacgctcgtg gagtttgcat ggctgacaat
    15601 tcatcgaccg ggacgtttcg gatggaccta cccaaggacc tggtgtcggc cgcacagcgc
    15661 gtgccgcaac cgccgggcac tgagttcaag gtcgaggggt ggcacaactc ctggttggcg
    15721 cgcatttccg agatgttggt cggcaaggac tgaacctgcc gccgccggaa acacctgtcg
    15781 attcatcgca tataagtcaa atttagcgga cttatccgct gcatcggcat tactatggcg
    15841 cacggaggta gaattccatg cgcttggctc gccctgttta cgaaagcttg ccgtatcttt
    15901 atatcgtgat cggcggattg tcgatttttc tgttttacct cgatcccatc ggcccacgcg
    15961 ccgtcatcgc cttcctcatc ggcgtcacgg tcgagatcgc ggcgctgacc ttgctgctgc
    16021 ggcgcaagga ttaccgcgca ttgagccgcg aatacaccgg cgagagcatc gactttccgt
    16081 cgacgctcca cggctgatcg ctcaaggctc ttcgtcctct tcatcatcgg gctcgccgcg
    16141 caggcgcggt gaacgttcgt cgatgtacat gatttcgtgc tgcccgatct ggaccacgtc
    16201 gccgtcgttc aagttgtact gacgcacgcg cttcgactgc acgaaaattc cgttggtact
    16261 gttgagatct tcgatcacgc acgagtcggc ctgcgtcacg atctggcagt ggtggcggct
    16321 gacgaatttg ctgtcgattt gcagatcgtt ctccggagtc cggccgatga ccaagcggcc
    16381 cggcttcagt tcgcgttcga gcaccgtttt gccggcgctt gccagaagaa tccgtcccac
    16441 ggtgggcgcg acctcgacgc tcggggccgg caccggctcg ggagcggcgg gcgccgcggc
    16501 ctggacttcg cttttggcgg gggcttcggc ctggggctcg gattcgacct cgggctccga
    16561 ttcaggctct ggttcgggtg ccggttccgg ttcgggtgtc ggcgctcccc gcgctccctt
    16621 gccgaacggc acccaacgca gctcctcgat cgccgcttcg agatcctgct tcgacaccgt
    16681 atcgcgggcg cggccgtagg cggccatcag gcaggtgtcg cacagcgtat tgatcaggcg
    16741 cggcacgccg ccggtgtatt tgtaaatcgt cccgtaggtg ccttcctcga agatgcgccg
    16801 cccctgggaa ccggcgacct ccaggcgatg atcgatgtac gcgatggtct ccgccttggt
    16861 gagcgcggcg aggtgaaatc gcaagcgcac gcgctgcgcg agctgcacga gttccgagga
    16921 gttgagtttg tcgttgagtt cgggctgccc ggcaagaatg atgcgcagcg ccttctcctt
    16981 ggtcgtctcg atcccggaga gcatgcgaac ttcttcgagt acgcggctgg agagattttg
    17041 agcttcgtcg acgatcagca ataccttgcg gcctttgata tgttgttcga ccaggaattg
    17101 attcaaggtc gccaacactt ccggcttctt catgttgaac ggcgaaaaac cgaactgcac
    17161 caggacggtc tgtagaaatg ccgtcggcga caattgcgtc tggttgattt gcgcgaccac
    17221 ggcatcggtt tgcagctcgc gcaaaaacgt ttcgatcagg gtggtcttgc ccgcgccgat
    17281 ttcaccggtg atcacgacga agccgtcggt gaaccagatg gtcgactcca tgtacgcctt
    17341 ggcgcgcgca tgctgtttgc tcaaatagag gaattgcgga tccgggctca aacggaacgg
    17401 cagctcgtga agcttgaatt gttctaaata catggtcatc ggcagtctac gggattggtc
    17461 acgtccattg gatcgattct aatgcggccg caagcgccga attgcgctcg gccgcacgtc
    17521 ttttgagaat agtacagtga ccgagcttgc ggccggggcg cggctccttg ccatagtcgt
    17581 ggaaagccag cccgtcgatc gccaaaagcc gttcgcggtc gggcatttcg cccagaaaat
    17641 tgatcatcgc ggcgtgcccc aacggccgcg tgctgccaag cggcaggtcg caaatcgcgc
    17701 gcagatggtt ttcgaactgg ctggtcacgc agccctcgat ggtccaatgc ccggaattgt
    17761 ggactcgagg ggccatctca ttcgcgacca ggcggccctt gacgacgaaa aattccaccg
    17821 tgagaacacc gacataatca agcgcattca tgagccgttt caagtatgtc cgtgcgctgc
    17881 gctcgagtcg cgcgtttgca aagggtgcga cattatagtg cagaatgccg ccgccgtggc
    17941 tattggccgc cagcggatag aaaaccatgc gaccggtcgc cgagcgcgcg ccgatgatgg
    18001 atacttcgcg cgagaaatcc tgaaacttct cgtatatcaa gtcgcggccg ccgatggcgg
    18061 cccaggcctc gccgatctgg gacgcctcgc gcaggatgaa ttgccccttg ccgtcatacc
    18121 ccaagcgccg cgttttgagt acgccggccc cgagtttttt caccgcccgc gccagttcga
    18181 gttcactatc gacggcggcg tgcgcggcga cggggatgcg taatttctcg aacaatgcct
    18241 tctcgctcag acgatcctgc gacacctcga gtgcggcacg cggcgggcgc actttcgtca
    18301 acttctcgag cggtgcgagc gcgcccgcgg agatattctc ccaatcgaac gtgagcacat
    18361 cgctacagcc ggcgagtgtc gcgagctgcg tcggatcctc gagttcggcc gtcagtatcg
    18421 gcgccacttg cgcggccggg gccttggcgc tgcgatcgag aaacaagcag tgcatcccca
    18481 gcgggtaacc ggcgagagcc agcattcggc ccagttggcc ggcaccgaca atgccgacgc
    18541 gcgataacct caagggctgc gcggatccgg cttcgcgagg accgccgccg tctgggcctg
    18601 gcgaaacgcg tccaaggcgg cgcggatctc ggtgttttcg ttggcgagga tcgcggccgc
    18661 aaacagtgcg gcgttcttcg cgccggccgc gccgatggcg aaggtcgcga ccgggacgcc
    18721 gctcggcatt tgcgcgatgg acagcaatga atcaaggcca ttcaagccct tggattgcac
    18781 gggtacgccc agcaccggaa ccgaggtctt ggcggccgtc attccgggca gatgcgctgc
    18841 gccgccggct cccgcaatga cggctcgcag accgcgttcc cgcgcgctcg ccgcgtactc
    18901 gaacaacagg tcgggtgtgc gatgcgccga gaccacgcgc acctcgtacg cgatcgcgag
    18961 agaatccagg atagcggcgg cgccctgcat agtttcccag tcggacgatg atcccataat
    19021 gattccgacc agggctttca tggcggctag tctagcaaat gctatccgat caactggcgc
    19081 agaaaggcga aaagctcgcg cgcgccgacc ggcgagcgct gctccacgcg ctcgcgctca
    19141 gccttctcca ggagtttggt cagggcggag cggtccgctt gcgggtactc ctgcaacagc
    19201 tcatcgaggc ccgcaacggg ctcggacagc agccggtcgc gccagcgctc gagggtctgg
    19261 aaatgccgaa tttccaagtc atggcgctgc ttgcgttccg ccagcttggc aagtaccggc
    19321 tcggggtcga tattgcgcat caacttgccg atgtattgcc gctgccgaac ctgcgcccca
    19381 tgggagggca gacggcgcaa atcacgcagt gcaacgaaca ggttgtcagg caagtccagc
    19441 gctttgatct cctggtccgg gagagccgac aatttaacgc ccaactcctg caatgaagcc
    19501 gcctctcgct tgcgggcgct cttgctgcgg cgctcgagct ccgattcgcc ggactcgtca
    19561 tcgaatgtgc cgccttcagt gtcgctcgtc atatcgccca tgctacaccc gcgcccggta
    19621 gaatcgccgc tcagaatcct caatgaaagt atttggagat gccgatggcc gtgtccagca
    19681 atcccgtccg cctggcccaa ggagacttgg aatcgattat cgagcgcgcc ctggaagagg
    19741 cgcgcgcgcg cggtgcaagc caagccgagg cggccgtcag ccaggatacg ggcctttccg
    19801 tcggcgtgcg tctgggggag gtcgaaacgc tcgaacacca gcgcgaccgc agcatgggaa
    19861 tcacggtgta tttcggccag cgcaagggct cggccagcac cgcggacttc tcgctggagg
    19921 cggtgcgggc gaccgtggcc aaagcctgca gcatcgcccg gttcacggcc gaggatgcgt
    19981 gttccgggct cgcggacgcg gccctcatgg cgcgcgcccc ggcggatttg gatttggcgc
    20041 atccgtggaa tgtcagcgct gatcgggcga tcgagatcgc caggacctgc gaggccgcgg
    20101 cccttggctt cgatgcgcgc atcaacaatt ccgagggtgc atcgctcgcc acgcatcagg
    20161 ggctgcatgt gtatgggaat acccacggtt tcgtcggcgg ctatcccacc acctctcatt
    20221 cgttgagctg cgtcgtgctc gccggcgtgg gcgaggatat gcagcgcgat tattggtaca
    20281 gcacgtcgcg cgattggcgc gatctcgagc ccgccgaaca gatcgggcgc gagagcgcgc
    20341 ggcgcacgat agcccgcttg aacccgcgca aattgagcac gcgccgcgcg ccggtcattt
    20401 tcgtgccgga aatcgcccgc ggtttgatcg ggcatttcgt gggcgcgatt cgcggcagca
    20461 gccagtaccg gcaatcctcg ttcctgttga actcagccgg tgaacaggtg tttccgcgcg
    20521 gcttctcgat cgcggaacgg ccgcacatcc gcaaagccat gggcagcgca ccattcgatg
    20581 atgagggcgt ggcgactcac gacagagaac tgatcgccga tggggtgctc accggttaca
    20641 tactgtcgag ttactcggcc cgcaaactcg gtctcaagac caccggcaat gcgggcggtg
    20701 cgcacaatct cctggtcgcg ccgaacctcg ccggcggcat ggcggcaatg ttgtcgcagc
    20761 tcggcaccgg gctcctggtg accgaactga tggggcaggg cgtcaatggg gtgaccggcg
    20821 attattcgcg cggcgcggcg ggcttctggg tcgagaacgg ctcgctacag tatccggtgg
    20881 cggagatcac catcgccgga aatctgcgcg acatgctcaa aaacatcgcc gccgtgggcg
    20941 acgatgtcga tgcgcgcggc ggcacccgcg tggggtcgat actgctgcag gaaatgacga
    21001 tcgccggcga atgaggccgg cgcgaaccta ggcctgcggc gcgatcagat ccttgagcga
    21061 ctgactgccc agccgctcgc gcatcgcgcc ctccacctcg gccatcactt gcgccgcgtt
    21121 gccgactcga tgcagctcga ccgtcagccg gctgatttgc agcgtgcgca cggcgtgcag
    21181 aatatcggcg agcagaattc cggccggatc gcgcccgggt acgaattgct ccttttcggt
    21241 cgccacgatc agtccgccgc gctccagacg cgccagcacc ggcgcgagcg cgatgcccgg
    21301 gacatcgagt tcggcggcga ggcggccggt gttccaatag cctcgaccgg cctcgaagtc
    21361 gcggcctatc aggtacatga tggacagcgc cacctgctcg cgatcgctgg cggtgagctc
    21421 gatggcctcc tggccgtgcc gcagatattg gggaaactgc acgtagaacg cgagctgcgc
    21481 gccgatcagc aaaatgagcc aactcaagta cacccagatc aacgtcgtca gcacgatggc
    21541 gaagccggtg tagaccgcaa ccagctgcga ggaatacacg agcacggccg agaacacctt
    21601 gcccaccagc gcccagatga tgccggcggt gacgccgccg atcacggcgg cacgaaattg
    21661 cacgtgagtg ttgggaatat aggaatacat gaacgtgaac accgcggcga cgatgatgta
    21721 gggcacgacc ttggcaaacg cacccaacag cgccgcgatg ggcgcgaccg cctgcagcca
    21781 ttgtgcaaag ggactgtgct ccgcggaacc caacagtccg atcgcaacgg ccagcaaaat
    21841 cgggcccacg atcatgacgc tcaagtattc gctgaagcgc cgcgcaatgc tgcgcggccg
    21901 ctcgacgcgc cacacgaaat tgaaactgcc ctcgattttc tgaatggtgg cgattaccgt
    21961 gtagacgagc gacgccaggc cgatggagcc gagcaactcg ccgcgcatat tggtgacgaa
    22021 ttgcatcacg ctatcggtca gctcatccgc cgcgctgccc atggggcgga agaattcgtg
    22081 cagcaggaat ttgaggtcgc cgcgcgcgcc gatacccttg agaatcgcga aactgaacac
    22141 catcagaggc accagcgaca acagggtggt gtatgccaag ctcatcgcgc gaatattgat
    22201 ctcgccgccg agccagtcgc gcagcagcgc cgccgggtag cgcagccagc gcaacggggc
    22261 gccccacggc ggaccgatgc tgcgcgactt ctgaaatagc aggtcctcga gccgttccag
    22321 ccacgtgagc atggcgggcg cttacgctgt cagcagcttg agcgcctcgc gatagttatc
    22381 gctggtgcgc ttcacgacat ccggcggcaa atgcggcgca ggcggttttt tgttccagtg
    22441 cagcgactcc aagtaatcgc gcacgaattg cttgtcgaag ctcggcggac tgatgccggg
    22501 cttgtaggat tgcgtcggcc aaaagcgcga ggagtccggc gtcagcactt cgtcgatgag
    22561 catcagacgt cccgccgcat ccacgccgaa ttcgaacttg gtatcggcga tgatgatgcg
    22621 gcgggcgcgc gcgtgctcgg ccgcaaatcg ataaatgtcg agcgcgatgc tgcgaacttg
    22681 cgcggcgtat tcgcgtccga tcaacttgac catggcttcg aaatcgatat tttcatcgtg
    22741 ctggcccgag gcggctttga aggccggcgt gaacaggggt tcgggcaagc ggtccgccat
    22801 gtgcaatccg gcgggcagtt cgatgccgca gaccgcgcca tgcaacttgt agtccttgta
    22861 acccgagccg atcaaatagc cgcgcaccac cgcttcgatc ggcaacgcct tgagcttctt
    22921 gacgaccatc gcgcgtccgg ccagctgcgc acgttccgcc gcgtcggcga ccacgtcctc
    22981 caccttgagc gcgcacagat gattggcaac catgtgctgg gtcttcgcga accagaacaa
    23041 ggaaatctgc gtgagcacct cgcccttgaa cgggatcggg tccggcaaca caacgtcgaa
    23101 tgccgacagg cgatcgctgg tgacgatcag cagatgctgc gcgtccacat cgtagatatc
    23161 gcgcactttt ccctggtgaa tcttcttcag gccgcgcaag cgcgactcga aaagcggtgc
    23221 cgaatgcgaa acctgttgca tcgttgcgcg ccctgccggc tgctaccatt gaggagtgga
    23281 aaggtcggcg attgtggcgg gacaagatgg ataggtcaac gcgaatgagt tggaaaggca
    23341 tgtttttcgg ggctgtcgtc ggctttttgc tcagccggca ggtctgggga gcggtaatcg
    23401 gggccgtgat cggacacctg ttcgatcaat ccttgaatct gggcgccgcc ggcgcgggcg
    23461 ccggccgcgg gcaaagttcg ctatcgatcg ccgatgagtt ttttcgcaca accttcgagt
    23521 tgatgggcca cgtcgccaaa tcggacggcc gagtgaccga ggcggagatc gatgcggcgc
    23581 gccgtctgat gcacgagctc aagctcgggc cgcgcgagat cggcattgcc atcgagtgtt
    23641 ttcgcagcgg taaatccacg gcctacgatg ccgaactcag cgtcgagagg ctgcgcgagg
    23701 cctgcggcca gcgttacgat ctgctgcgcg ccttcatgga attgcagctg cgcgcggcgc
    23761 tggcgggcaa cggcatgtcg ccgccggcgc gggcgatctt gacgcgcgtg gccgaacgcc
    23821 tcggcatgtc gggcgtggaa ttcgccttca tggaggcggc gctgcgggcg ccggccgcag
    23881 cggcgccgcc ggcgggtcgc ttgccgcgtg ctatgcggaa ctggaaatcg acgccgctaa
    23941 gactgatcaa gaagtgatca aagcgtatcg acgccaaatg agccgccatc atcctgataa
    24001 actggtggcc aacggcttgc ctgagtccat ggcgcaagtg gcgaaagaaa agactcagcg
    24061 cattcaggaa gcctatgagg agatccgcgc ggcgcgcggc atgcgatgaa ggagtgagga
    24121 tgacgagcat tatttcgccc tcgattctgt ccgctgattt cgcccggctg ggggaggagg
    24181 tcagggcggt gctggcggcc ggcgccgact gggtgcactt cgacgtaatg gataatcact
    24241 acgttccgaa cttgaccatc ggaccgttgg tgtgcgaggc gttgcgcaag gcgggaatca
    24301 aggcgccgat cgatgtgcac ttgatgattt cgccggtcga cgcactcgtc ccggacttcg
    24361 ccaaagcagg cgcgagctac atcagctttc acccggaggc gacccgacac gtggatcgca
    24421 cgattcagct gatccgcgat tccggatgca agcccggcct ggtgttcaat ccggcgacgc
    24481 ccttgaactg gctcgactac gtcatcgaga aaatcgattt ggtgctgatc atgtcggtga
    24541 atcccggatt tggcggccag aaattcattc cgcaggccct gcataagctg gcggaggcac
    24601 gcgcgcgcat caaggccagc ggccgtgata tacgcctgga aatcgacggc ggcgtgaaag
    24661 tcgacaatgc ggcggaaatc gcccgggccg gcgccgacac cttcgtttca ggctcagcga
    24721 ttttcggcag ttccgactat gcggcgacaa tcaaggcgat gcgcgcgcaa atcgcctgcg
    24781 gcgcaaccga agcctagtcg cgccggctac tcgtcgtcat cctcatcgtc gtcatcctca
    24841 tcatcatccg gcgtctcggt gcgcaggctc ttcggggcgg ccgcggcgct cgagcccgcg
    24901 ccggctttca ccgggacgat caatggccgc gtctttactt tcggacgggc gttgaatacg
    24961 acgatggtct tgccggtggc gcgcagtaga ccttgttctt ccagcacttt gagcacgcgt
    25021 ccggccatct cgcgcgaaca gccgaccagc cgcgacaact cctgccggct cactttgatt
    25081 tgcatgccct cgggatgcgt catggcatcg ggctcgccgc acagatccat gatggcgtgc
    25141 gccacacggc cggtgacgtc gacgaacgcg agatcaccca acttgcgatt ggtgcggtcg
    25201 agccgcgtcg ccagttgggt cgccaactcg aagatcagtc cggggctttc ggcggcaatc
    25261 tgccgaaagc gcgggtaggt catttccgcc agttccgatt gctgccgggt gcgcacccag
    25321 gcgctgcggg tcggctgctc gtagaacaag cccatttccc cgaaaaactg gcccttgttc
    25381 agatacgcca gcaccatttc gttgccatcc tcatcctcga tcatgacctc gaccgaaccg
    25441 gagatgatgt agtacaaaat gtccggtaaa tcgccggcgt ggatgatcac ggtcttggaa
    25501 gggactgtcc ggatacggca gtagctcaag aaacgattga tcgctgggat atcgcttaag
    25561 gcctttgccc cttcttccat ggtctctccc taaggttctt gatcgtctgt taaacgtcga
    25621 tgcaggcgca cgttttatta cataagtccc ggaatcgcaa gcactaacgg ctattcgtta
    25681 cagccaatag gagccgtgag tcatgagccg ggaggtcaac tgcatcgcta ccttaagcgc
    25741 aaatgggggc tgtttgcctc ccaaggaact cgcctggacg ccatgctgta tctcatcgtg
    25801 cgtcatctgt tcgagaatgg ccagactgcg cgggtccgcc tggccgagcc taagcatgtg
    25861 accgcgcaaa tgagcctcca cctgccgttc ggtttcggca acgaacccca agctcgcacc
    25921 gcgcccgaag gcacccgcga cggcgccgat cgcgaaggaa ccgacgtacc agagggggtt
    25981 tagcaggctg gtgcggccgt tgagctcgcc gagccgcaat tcgcaccagg cgaggtggtc
    26041 ggcctcctcg gccgccgcat ggtgcagggc cgcggccacg atgggatcac ccgccgtcag
    26101 cgcctgtcct tggtagagtg cctgggccgc gacctccccc gcatggttga cgcgcatgag
    26161 gcgcgccgac tcggcgcgct gcgccgccgg cagcgatgcc tcttgctgcg ccgcgggcac
    26221 cggccgcaag gaccgcgcag gcgcgcctaa caccttgatt gccttgtcta tttctcccaa
    26281 gacgcgatcg attaggctaa aactgcgggt gctcatagcc acactatatt ataagggtga
    26341 gccggttttc gcggggctgg catctacgcg cgccgttctg tacactttcg cgcctttcgt
    26401 taacgacctg aaacagctga gtattttcat gtataccgta ttttcgaagc ccgaagaaac
    26461 ccgtggcgac tggtttctgg tcgatgcggc cggcaagact ctcggccggt tggcctcgga
    26521 gattgcgcac cgattgaagg gcaagcacaa gccgaattat gcgccccacc aagaccttgg
    26581 cgatcacatc gtggtgatca acgccggcac ggtccgggtc acgggagcca agctcaccga
    26641 caagttttat catcagcaca cgggctatgt aggcaacctg aagagcatct cgctcggtaa
    26701 gctgctggtc gagcatccag agcgggctgt cgagttcgcg gtgaagggca tgctgccgaa
    26761 gggaccgctc ggccgtcgca tgttcagaaa gctgcatgtt ttcggcggca aggatcatac
    26821 ccaccaagca cagcagccca aggcgctgga aatttaatcg agggatttga gctatggcat
    26881 caaagacagc cgtcaattct ttctacggca ccggtcgccg caagacctcg agtgcacggg
    26941 tgtacctgcg tcagggcacg ggcagggtct ccatcaacgg ccggtctctc gatgagttct
    27001 tcggccgcga aacgggccgt atgatcgtgc aacagccgtt cggcgcggtg cagctcggcg
    27061 gcaaattcga tgtcgacgcg cacgtcgaag gcggcgggat caccggccag gccggtgcga
    27121 ttcgccatgg catcactcgc gcgctgatgg cttacgacga gagcctgcgc gcgccgttgc
    27181 gcaaagccgg tttcgtgacc cgcgatgcac gtgaagtcga acgcaagaag gtcggacgcc
    27241 gcaaagcgcg tcgcggcacc cagttctcca aacgctaatc gcggcaacgc ctagcgcggc
    27301 ttgtggggga tcgtctagtg gtaggacaac ggtctctgac accgtttacc taggttcgaa
    27361 tcctagtccc ccagccatat agggcccgac cgtgggccct ttgtctcaaa cactgcacgg
    27421 gtagcaatgt cgggcgaacg aaattcgagt ggacggctcg cgccattggc gcttgcagcg
    27481 ctcggtgtcg tgttcggtga cattggcacg agcccgctgt atgccatgtc gtcttgcttc
    27541 accggtccga acattgccgt cacgcccgcg catgtgctcg gcgtcttgtc gctgatcttc
    27601 tggtccttgg tcctcgtcat cctgctcaag tacgtgagcg tggtattgaa cgccgacaac
    27661 aagggcgagg gcggcgtatt ggccctgacc gcgctcgtga tcaataccga gcgcgaaagt
    27721 ccgcgccgca aactgtacgg tacgctcggc atcctgggcg cctcgttgtt cttcgccgac
    27781 ggcgcgatca cgccggcgat ttcggtgttg agcgccatcg aaggtctgca cgtcgcggcg
    27841 cccgatgcgc aaattccggt gctgccgatc accgcgatcg tgctggttgg attgttcctg
    27901 atccaacggc atggcacggg taccgtcggc aaattcttcg gtccggtgat gctcttctgg
    27961 ttcgcgatca tagcggccat gggtgtcagt tggatcgtgc gcgaaccgaa ggtgatcctg
    28021 gcactcgatc caacgcgcgc gctcgcatta ttggcgcagc atcaggtgag ctcgctcgcg
    28081 gtcctcgcgg gcgtgttctt gacggtgacc gggggcgaag ccctgtatgc ggacatgggt
    28141 cacttcggca aagcgccgat acggctcggt tggatttgca tcgtgatgcc ggcgctggtt
    28201 ctcaattatt tcggtcaggg tgcgatgctg atcgaggatc cgaagtccat cagcaatcct
    28261 ttctacctga tggcgcccgc atggtcgctg tggcccttgg tcctgctcgc gacggcggcg
    28321 accgtcatcg cctcgcaggc ggtgatctcg ggcgtgttct cggtcaccac acaggcggtc
    28381 agtctggggc ttctgccgcg cctcaaagtc gaacattcct ccgcggacaa cgcgggacag
    28441 atctacgtgc cgaccatgaa ttggatcctg atgatcgccg cgatcgcgct ggtcatgggt
    28501 ttcggcagtt cggcggcatt gggcggcgcg tacggcctcg cggtgtcggg cgccatgacg
    28561 atcgtgaccc ccttgacctt gagtctgatc aaatcgcgca ccggcaagga cagccgctgg
    28621 ctgcgcaccg gcctgtcgct gctgttcatc gtcgacatcg ccttcctgtt ggcgaatctg
    28681 accaaactcg aggacggcgg ctggttaccg ctgtcctccg gactgatcat ctactggctg
    28741 atgcaaacct ggcaacgcgg ccgtgcctcg gtcatggcgc ggctgttgcg cgaacagaag
    28801 ccggtgcgcg actttctcga ggacttgaag cgcgcgccgc cggtgcgggt gccgggtacc
    28861 gccatcttcc tcgaccagaa ggcctctggc attccgcgtg ccctgctcaa caacctcaag
    28921 ttcaaccgcg tgatgcacga gcgggtggtg ttgttgacgg tggtgacccg cgagcagccg
    28981 cggatagcga cctcgaagcg cctgaccgtg acgccggtat gcgagggcat cgtgagaatc
    29041 gtggcgcagg tcgggttcat ggagaaaccg aacatttcgg agattctgcg cgaggccgac
    29101 gcacacggcg taccctacga tcccgagtgc accacctatt tcttgagccg cgaggaattg
    29161 ctggcgggac agcgctcgga cctgccggcg ctgcggcgcc gagcgttcat gcagatggcg
    29221 cgcagcgcac aagtgatcgc cgactactat ggtctgccgc ccaatcgggt gatcgagatc
    29281 ggcacccgca tggagatcta ggccggtcgg caatcaaggg tcttgacgcc ggcatcgccc
    29341 gccacgatca ccagatccgc gcggcgcagc gcgaacaggc cgacggtcac cacccccgcg
    29401 atttgattcg cggcggattc gaaggactgc ggttcatcga gctcgaggtt gtggacatcc
    29461 aagatgacgt tgccgttatc ggtgataacg ccctcgcgat agacaggttg cccccccatc
    29521 ttcaccagtt ggcgcgcgac ataggagcgc gccatcggaa tcacctcgat cggcagcggg
    29581 aacttgccga gcacctcgac cagcttgctg tcgtcgatga tgcagacgaa tgtcgcggcg
    29641 gcggcggcca ggaccttctc gcgagtgagc gcggcgccgc cgcccttgat cagcacgccg
    29701 tcggcggtcg cttcatcggc gccgtcgata tagaggctca agggtccggc ggaattgaga
    29761 tccagttcga gaatgcccga ggtgcgcaat agttcggaag tctggcggga actggatacc
    29821 gcgccggaaa gctgtatttt ccgctgtttg agtgccgcga tgagctgcgt caccgtcgat
    29881 ccggtgccaa tacccaacac catgccgggt tgtacgtaag caagagcggc ttcggcggcg
    29941 cgacgtttgt tttccagtga cgacaaattt ccgaccctca gaaacaactg tgcccgctta
    30001 cgcgggcaca gttgattact ttcgaaggct tactttttct tagcggcctt tttcttagct
    30061 accttcttct tcgccttctt tgctttcgct gccatgatag aaactccgtt atcgggttag
    30121 tccggggtcc gagtatatac acgattaatt aatttacaag caatacgtca cccaacatgc
    30181 tggacaatgt gctagcgatg tgagcgacgt cactcaagtg tcagcacgaa ctcccagtcc
    30241 ttcgctgcca ccggcatcac cgataaacga ttcccacgac gcaacaaaac aaaattcttg
    30301 agcgactttg cggcgtgctg cttgagctca tcgagcgtga tgatgcgctt caacttgcgc
    30361 accaacttca catcgaccac gaaccagcgc ggcgcatcgg tgttggagtc ggcatcgtag
    30421 tgatgatgct tgggatcgaa ggccgtctta tcggggtagc cctcgcgcgc gatgctgacg
    30481 atgccggcga tcccgggaac ctcgcaactc gagtgataga aaaacgccaa atcgccctgt
    30541 ttcatagagt cccgcagcat gttgcgcgcc tgataattgc gcaccccatc ccatgcggtg
    30601 gtctgtttgg gcgccttgct caaggcgtcg atgctgaagc tgtccggttc ggatttcagc
    30661 agccagtaat tcatgaagca ccccttgcgt aatgatgtag tccggattcg gtcgccagcg
    30721 agtcgagctt cacatcccac tcttgcgcaa aactcgtcgc ggtgcgctga caatcgaaag
    30781 cgagtcccac caggcgcggc cccgcccagc tgcggcggtg gcggcgaaac tcgagcgctc
    30841 gatcatagaa tccgccgccc atgcctaagc ggcgaccttg cgcatccacg cccaccaagg
    30901 ggagcacgat cagattcagc cagcggggcg gcagcggctg cgcccgcatc gaggggacgg
    30961 ctatgccgta ggtgccgcgt tgcagtttgc ccgagagcgg atagaaacgg atgcggctgc
    31021 ggcgccgatc gacgatcacc gggacccata tgccgacacc gcggcggcgc gccgcagcga
    31081 tcagcgcggc ggtatcggtt tcccgatcga agggcagata caccgcgatg cgttttccgg
    31141 ctctgaacat cggcaatccg gcgattgctt tggcggcgag gtttgagcgc tgcgcgtgaa
    31201 cggccgtatt caaggaacgg cgctggtttc gcagatgttt acgcagtgtc ggtttcattc
    31261 acgggcaaag gagatggcgc tacccgcaga tgccgttttg gaccgttgct cccgacctga
    31321 gcaacaggtg gggctaactg cggcacttaa ggctttccgc tcgaggcgga cttgcacaat
    31381 gctgccagca agcaatggcc cctggggttt aaaacttagg gtcgagaggt aacccgatcc
    31441 gcatcgaaca ccgcaggcag cgccaaactt aaccattcga ggagggtgcc cgattcaggg
    31501 catcaaagct cgagctgttg acccttctcc aaagccgact ctacgcgctc gcgcaggata
    31561 cgcaagcgtc cacccacgtc ggattcgaga tcgccatcac gattgcggaa gcgaatcaat
    31621 tcattcgcca tattcagcgc cgcgatcacg gcgatgcgat ccaggccgac gactttgccg
    31681 ctatcgcgga tctcgcgcat tttgaggttc aagaattcgg cggaatcgag caggtcggaa
    31741 cgctcttcga tcggacaggt aatgtgatac tccttctcga gtatccgcac gctgacccgc
    31801 gcctgattgt cgctcatgcc tgttccatcg atttcaagcg gccgatcatc gcttcgaccc
    31861 gcgcacgaac ctgttcattc ttttgcagca gactggcgcg ctcggcggtc agcgtttcct
    31921 gccgctgccg caacgcgcgg ttctcctcct tgatctggct gaccgtcaaa atgagatcct
    31981 cgaggcgctt ttccagacgc ttgagctcga gttccac
//
LOCUS       DASWZT010000026         5373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_24461, whole genome shotgun sequence.
ACCESSION   DASWZT010000026 DASWZT010000000
VERSION     DASWZT010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24461"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="VGE92_00765"
     CDS             <1..1200
                     /locus_tag="VGE92_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1312370.1"
                     /translation="EYLVDGPFLGNDFVAPPPPGTPYADIFVSRDQPSSAGTTSLAIN
                     RSDLTSTFEVGGYQNVLTTGLEVSKEQSNVSRFYNGLDDIAPTPLLHPDPFDTPPSPL
                     TAYSNPKGRGRDISPYVLDSIGFGPHWDVDLGLRWDRFASSFSERFSGTAFARVDTFV
                     SPRAAVIFKPDEAQSYYLSYGTSYNPVIEYLIVAPSDQSLAPEKNSNLELGVKIKIWN
                     AKAQLTGAVFDTRVSNARNSDPDDPTVQQAPYTQRVKGLELGFNGYVTGIWEVTANYT
                     HLNDRITASDDALAIGKLAPNAPHDAFNLWSTVEPTTAWTLAGGLTAMSHRYADNDNT
                     AGVPSFVVFNLMSSYQVNEHLKLQLNLNNVTDKLYFTGFYYTGAAENHALPAPGRTLV
                     GLASYRF"
     gene            1214..1978
                     /locus_tag="VGE92_00770"
     CDS             1214..1978
                     /locus_tag="VGE92_00770"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1312371.1"
                     /translation="MPDSASITALLAQMSAPRLNALFAQEPKRAADWVHVTAAEGLPQ
                     AQLCYGRMLLEGTGRPKDPAAALDWFRRAAARGDIEALNMVGRCLDNGWGTAVDPKAA
                     AAHFRLAAAAGHAWAQYNLGHLYLDGRGVARDRTQAFAWYLKSAEQGHERAMNLVGRC
                     CEEAWGTARDPAAAADWYRQSAQAGYFRGQYNWASVLLKNGREDEAAAWFERAITDSP
                     AALRPAMRAAVRIALARAGSSGALRALASRLTSAAE"
     gene            1983..2666
                     /locus_tag="VGE92_00775"
     CDS             1983..2666
                     /locus_tag="VGE92_00775"
                     /inference="COORDINATES: protein motif:HMM:NF003974.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HEY1312372.1"
                     /translation="MLLHVAKLLNDDQLAAMRRTLDATAPWVDGRVTAGHQGAAVKNN
                     QQLEETSALARELGNLIIGELERNADFISAVLPHRVYPPMFNRYSEGMQFGTHVDGAV
                     RTLPGTGHKLRTDLSATVFLAPPESYDGGELLIEHDVGRESVKLGAGDMIVYTSTARH
                     RVSPVTRGVRLASVFWIQSLIREDSKRQLLFELDRTIQQLTALNSDPESLVRLAGHYH
                     SLLRLWTEI"
     gene            2728..4050
                     /locus_tag="VGE92_00780"
     CDS             2728..4050
                     /locus_tag="VGE92_00780"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY1312373.1"
                     /translation="MRMTTCALGLAAAIFTHASLAEPAPVAAPIVLKAARLFDSVSGK
                     LVEHGVVVISGKKIEAVGSDAQIPANAEVIDLGDATLLPGFIDAHVHLSEESSPDWYH
                     DFYSEIMRFPAEQALYGAHYAKLTLEAGITTVRDVGSVDYIALGLRNAIAAGMIPGPR
                     MLVSNYAIGSTGGHADQDPYPAQRIAVAGPIKGVCNGADQCREAVRYQIKYGADVIKF
                     MPSGGVLSLSDPVDNAQLTQEEMNAIVSEAHNWGRKVAAHCHGDKAAKMAIAAGVDSI
                     EHGSFLQDDTLMEMKKKHVYLVATLSAGDWVGSHLDTFPPAIAVKAKAAAAQMQQMFQ
                     HAVKIGVPLAMGTDAAVEPHGRNAREFSLMTKNGLAPALALMAGTAGGADLLGVADKT
                     GTLSVGKFADIIAVAGDPLSDMHATEHPVFVMKEGTIYVNRGLASRSP"
     gene            complement(4058..4537)
                     /locus_tag="VGE92_00785"
     CDS             complement(4058..4537)
                     /locus_tag="VGE92_00785"
                     /inference="COORDINATES: protein motif:HMM:NF019819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HEY1312374.1"
                     /translation="MSPPLQTVSHVDLPRYMGDWYVIANIPYFAEKNCFDSVESYALR
                     PDGDIDNWFPCRNKSFEAPMKRKANAVASVEDKSSNARWRVKFFKVISVQYLILDLDP
                     NYQWMAVGHPSRKYGWIMARTKTLPDATYDGILHRLGDQGYDTTKFQKVPQRPAATP"
     gene            complement(4603..4827)
                     /locus_tag="VGE92_00790"
     CDS             complement(4603..4827)
                     /locus_tag="VGE92_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312375.1"
                     /translation="MDVEDCLKKLQSLFTCAASDSAAAKARYLAMTQQGAPPAVLSRA
                     RFAWRQFEARKAAIVARMVALEELEEGAIA"
     gene            complement(5009..>5373)
                     /locus_tag="VGE92_00795"
     CDS             complement(5009..>5373)
                     /locus_tag="VGE92_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312376.1"
                     /translation="ASSALETVAAAWGNHALRHNPAGRIGKKRRRRTKMPGMNIGSVS
                     SGFGALQNAASGISRASQGISQDAQSIATGALNGAQANNTTSALADLSEQSVLAQISA
                     AALSIDNQTLGTLLNVKA"
BASE COUNT          984 a         1663 c         1752 g          974 t
ORIGIN      
        1 gagtacctgg tggacggccc atttttgggc aacgacttcg ttgcaccgcc gccgccgggc
       61 acgccgtacg ccgatatttt cgtctcccgc gatcagccga gcagcgccgg gaccacgagt
      121 ctcgcgatca atcgctcgga cttgacctcg acgttcgagg tgggcggcta ccagaacgtg
      181 ctgacgacgg gcctcgaggt cagcaaggag cagtcgaacg tcagccgctt ctacaatgga
      241 ctcgatgaca ttgcaccgac acccttgctg catccggacc cgttcgatac gccgccctcg
      301 ccgctcactg cgtattccaa tccgaagggc agaggccgcg acatcagtcc ctatgtgctc
      361 gacagcatcg gcttcgggcc gcattgggat gtcgaccttg ggctgcgctg ggaccgattc
      421 gcgtcgagtt tcagcgagcg gttcagcggc acggccttcg cgcgcgtcga taccttcgtg
      481 agtccacgcg ccgcggtcat tttcaagccg gacgaggctc agagctacta cctctcctat
      541 ggcacctcct ataaccccgt gatcgagtac ctgatcgtgg cgccgtccga tcagagcttg
      601 gcgccggaga agaacagcaa cctcgagctg ggcgtcaaga tcaagatctg gaacgcgaag
      661 gcgcagctca ccggcgccgt gttcgacacg cgggtcagca acgcgcgcaa ttccgatccg
      721 gatgatccga cggtgcagca agcgccttac acgcagcgcg tgaagggact cgagttggga
      781 ttcaacggct acgtcaccgg gatctgggaa gtcaccgcca actataccca cctcaacgac
      841 aggatcacgg cgagcgatga tgcgctcgcg atcggcaaac tagcgccgaa cgcgccgcat
      901 gacgcattta atctgtggag cacggtggag ccgacgacgg cgtggaccct cgccggcgga
      961 ttgaccgcga tgagccatcg ctacgccgac aacgacaaca cggcgggcgt cccttccttt
     1021 gtggtgttca acctcatgag ttcctaccag gtgaacgagc atttgaagct gcagctcaat
     1081 ctcaacaatg tgaccgacaa actgtatttc accggcttct actacacggg cgccgcagaa
     1141 aatcacgccc tgccggcccc cgggcgcacg ctggtaggcc tggcgagcta tcgcttttag
     1201 ggacaccgag atcatgccgg actccgcatc gatcaccgcg ctgctcgcgc aaatgagcgc
     1261 cccgcgcttg aacgcgctgt tcgcgcagga acccaagcgc gccgccgatt gggtgcacgt
     1321 caccgccgcc gaagggttgc cgcaggcgca gctgtgttat ggccgcatgt tgctcgaggg
     1381 caccgggcgg ccgaaagatc cggctgccgc gctcgattgg ttccggcgtg cagcggcgcg
     1441 cggcgacatc gaggccctca acatggtggg gcgctgtctc gacaatggct ggggcacggc
     1501 cgtggatccg aaggcggcgg cggcgcattt ccggctcgcc gccgcggcag gtcacgcctg
     1561 ggcgcaatac aacttggggc acctgtactt ggatggtcgc ggtgtggcgc gcgatcgaac
     1621 tcaggccttc gcgtggtatc tcaagtcggc tgaacaaggg cacgagcggg ccatgaacct
     1681 ggttggccgg tgctgcgaag aggcgtgggg cacggcgcgc gatcccgccg ccgccgccga
     1741 ctggtaccgg cagtccgcac aggcgggcta ctttcgcggg cagtacaact gggccagcgt
     1801 actgctcaag aacggtcgcg aggacgaagc cgcagcgtgg ttcgagcgcg cgatcaccga
     1861 cagtcctgcg gcgctgcggc cggcgatgcg cgcggccgtg cgcatcgcgc tggcgcgtgc
     1921 cggcagcagc ggcgcattgc gcgctctggc ctcgcgcctg acctcggccg cggagtgagc
     1981 gcatgctgct gcacgttgcg aaattactca acgacgacca actggcggcg atgcgccgta
     2041 ctcttgacgc gacggcgccc tgggtcgacg gccgggtgac cgccggacat cagggcgccg
     2101 ccgtgaagaa caatcagcag ctcgaggaga ccagtgccct cgcacgcgaa ctcggcaatc
     2161 tcatcatcgg cgagctggaa cgcaacgctg acttcatcag cgcagtgttg ccgcatcgtg
     2221 tgtatccgcc gatgttcaat cgctacagcg aggggatgca attcggcacg cacgtcgatg
     2281 gcgcggtacg caccttgccg gggaccggcc acaaactgcg cacggatctg tcggcaacgg
     2341 tgttcctcgc gccgcccgaa tcctacgatg ggggtgagct gctgatcgaa catgacgtgg
     2401 gccgtgaaag cgtcaagctc ggcgccggcg acatgatcgt ctatacctcg accgcgcggc
     2461 atcgcgtcag tcccgtgact cgcggcgtgc gtctcgcgag cgtgttctgg atccagagct
     2521 tgattcgcga ggattccaag cgtcagctac tgttcgaact cgaccgcacc atccaacaat
     2581 tgacagcgct gaattcggac ccggagtcgt tggtgcggct cgccgggcat taccatagcc
     2641 tgctcaggct gtggacagaa atataaccgc acggcatttt gcggtattct cagcgccctc
     2701 aattccaatt ataacgaggg acgactcatg cggatgacca cctgcgcgct cggtctggcg
     2761 gcggcaattt tcacgcacgc gagcttggcg gagcctgcgc cggtagccgc tccgatcgtg
     2821 ctcaaagccg cgcgcctgtt cgactcggtg tcgggcaagc tcgtcgagca tggcgtggtc
     2881 gtgatctcgg ggaagaagat cgaggcggtg ggaagcgatg cccagattcc ggccaatgcc
     2941 gaggtcatcg acttgggcga tgccaccttg ctgcccggtt tcatcgacgc gcacgtgcat
     3001 ttgtcggagg agtcgagccc cgactggtat catgactttt acagcgagat catgcgtttc
     3061 cccgccgaac aggcgttgta cggcgcgcac tacgccaagc tcacgctcga ggctggaatc
     3121 accacggtgc gcgacgtcgg atcagtggat tacatcgcgc tcggactgcg caacgccatc
     3181 gccgcgggca tgattccggg tccgcgcatg ttggtgtcca actacgcgat cggttccacc
     3241 ggtgggcacg ccgatcaaga cccgtatccg gcgcagcgca ttgcggtggc cggccccatc
     3301 aagggcgtgt gcaacggcgc cgatcagtgc cgcgaggcgg tgcgctacca gatcaagtac
     3361 ggggcggatg tcatcaagtt catgccctcg ggcggcgtgc tgtcgctctc cgacccggtc
     3421 gacaatgcgc agctcacgca ggaggaaatg aatgccatcg tgtcggaggc tcacaattgg
     3481 gggcgcaagg tggcggcaca ctgccacggc gataaagccg cgaaaatggc catcgccgcc
     3541 ggggtagatt ccatcgagca cggcagcttc ctgcaggatg acacgctcat ggaaatgaag
     3601 aagaagcatg tgtacctggt ggcgacattg tccgccggcg actgggtggg ctcgcacctc
     3661 gacacgttcc cgccggccat cgcggtcaag gccaaggcgg ccgcggcgca gatgcagcag
     3721 atgtttcaac acgcggtgaa aatcggcgtg ccgctcgcca tgggcaccga cgcggcggtc
     3781 gagccgcatg ggcgcaatgc gcgtgagttc tcgctgatga ccaagaacgg tctggcgccg
     3841 gccttggcgt tgatggcggg caccgcgggc ggcgccgatc tgctcggcgt cgccgataaa
     3901 accggcaccc tgagcgtggg taagtttgcg gacatcatcg cggttgccgg cgatcccttg
     3961 agcgacatgc acgccaccga gcatccggtg ttcgtgatga aggaaggcac gatttacgta
     4021 aaccgcgggc tcgccagcag gtccccgtaa agatgcgcta aggagtggcg gcggggcgtt
     4081 gcggcacttt ctgaaacttg gtggtgtcgt agccttgatc gccgagccgg tgcaatatgc
     4141 catcgtatgt cgcgtcgggc agggttttgg tgcgtgccat aatccatcca tacttgcgcg
     4201 acgggtggcc gactgccatc cattggtagt tcgggtccag gtcgaggatc aggtactgca
     4261 ctgaaatcac tttgaagaac ttcacgcgcc agcgggcgtt gctgctcttg tcctcaacgc
     4321 tcgccactgc gttggccttg cgtttcatcg gcgcctcgaa cgacttgttg cggcagggga
     4381 accagttgtc gatgtcgccg tcgggccgca gcgcgtaact ctcgacggaa tcgaaacaat
     4441 tcttttcggc gaagtaggga atgttggcaa tcacgtacca gtcgcccatg tagcgcggca
     4501 ggtcgacatg cgagaccgtc tgcaggggtg ggctcatcga ggtgcatccg ccgaagacca
     4561 gcatcgcggc cgacaaaaca gccgctgcgg acgatgcgcg aatcaggcaa tggctccttc
     4621 ttccagttct tccaacgcca ccatgcgcgc gacgatcgcg gctttgcgcg cttcgaattg
     4681 ccgccaggca aaacgggcgc gggacaagac cgccggcggc gcgccttgtt gagtcatggc
     4741 gaggtagcgt gccttggcag ccgccgaatc gctcgccgcg caggtgaata gcgattggag
     4801 ttttttcagg cagtcttcga cgtccatcaa gcatctcccg gaaagcaccg cttaagcggc
     4861 tttgccgaac tatgccgggg tgggacgctt gagtcgcccg ccaaacgcct cgactgcgta
     4921 taggcgaagt cctatagcgg gagcatcacg gaggagcgaa gcgcggaacg ggccggggct
     4981 gaggtaaccc ctgaaagcat cgggcttttc aggctttgac gttgagcaga gtgccgagcg
     5041 tctgattgtc gatcgatagc gccgccgccg atatctgcgc gaggacgctc tgttcggata
     5101 agtcggcgag cgccgaggtg gtgttgttgg cctgggcgcc gttgagcgcg ccggtggcga
     5161 tcgactgtgc gtcttgactg ataccctgcg aggcgcggct aatgcccgaa gccgcattct
     5221 gcagggcgcc gaatcctgag ctgacgctgc cgatattcat gccgggcatt ttagtccgtc
     5281 ggcggcgttt cttccctatc cggccggcgg gattgtgacg cagagcgtgg tttccccagg
     5341 ccgccgcgac ggtttcgagt gcggacgagg cgc
//
LOCUS       DASWZT010000027         2576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_286, whole genome shotgun sequence.
ACCESSION   DASWZT010000027 DASWZT010000000
VERSION     DASWZT010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2099
                     /locus_tag="VGE92_00800"
     CDS             <1..2099
                     /locus_tag="VGE92_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HEY1312377.1"
                     /translation="RVLIVHAVSQRRLTVDRSVNAVIDYFAEPRRWPGDGTVLAGLVT
                     YDRDALGACVDSLVERKILTDKSADGELAEVTAKLGATYGRDPLELLDSYRRSLKEGA
                     EAYWSAGVAQGIADFAGGKRRVDVVLFGDCDIQMEADFLRREASRRGLDLRVAAAFPD
                     DFRFVSEHEHDAVIIGALRSRHSLTMPPPEPNAAAHAAYIAEASHMLERLRERTAAPI
                     LIDNLPEPTVQPLGLAERGLSGHRNRFRLANLVLFDLVESYTDVHVVDVAAALGSAGS
                     ETMLDDGQVGFTHMGSPGWLLQRPDSEKAAVHGIFPDSAPLARAVSGDPYSREALMAR
                     AHADALTTVLGHDRKKCVIVDLDGILWPGVLAETGSPFAWDPAISGMFSHVGLYFGLH
                     EALLCLKRRGVVLACVSKNDEATVRELWRYPDHYPRSRLLTPDDFVTWRVNWNDKVEN
                     IRSIAGELGFALDAFLFIDDHPVERDRVRQRLPEVEVWGDDLFSLRRRLLNDPRLQLP
                     CITAESTARTDLVKAQLNRRHLRADSMNEGDYVASLQIQCRIGRETGTSKLSRISELF
                     QRTTQFNATGRKFTVTELNDLIEGPDTCVFSMEVSDRFGDHGLVGVAVIERGEITGLV
                     LSCRVLGLGVEHQFVRYMIETLKADLGDLSGRIVETPRNSPVRNIYRDLGFTQRGDGL
                     WRLELKAEPARNLAVA"
     gene            2416..>2576
                     /gene="rplK"
                     /locus_tag="VGE92_00805"
     CDS             2416..>2576
                     /gene="rplK"
                     /locus_tag="VGE92_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="binds directly to 23S ribosomal RNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEY1312378.1"
                     /translation="MAKKVTGFLKLQVPAGAANPSPPIGPALGQRGLNIMEFCKAFNA
                     QTQKIEKNTP"
BASE COUNT          466 a          788 c          819 g          503 t
ORIGIN      
        1 accgcgtgct gatcgtgcac gcggtaagcc agcgtcgcct cactgtcgac cgcagcgtca
       61 atgccgttat cgattatttc gcggagccgc gtcggtggcc cggcgatgga acggtgcttg
      121 ccggtcttgt cacttacgat cgggatgccc tcggtgcctg cgtcgattcg cttgtcgagc
      181 gcaagattct cacggacaaa agtgcggacg gcgagctcgc ggaggtgacc gcaaagctcg
      241 gcgcgaccta cggccgcgat ccgctcgaat tgctcgacag ctatcgccgc tccctgaagg
      301 aaggggcgga ggcctattgg tcggcgggtg tggcgcaagg cattgccgac ttcgccggcg
      361 gcaagcggcg cgtcgatgtc gttctgttcg gcgattgcga tattcagatg gaagcggatt
      421 ttctccggcg cgaggcgagc cgccgcggcc tcgatttgcg cgtagcggcc gcattccccg
      481 acgatttccg ttttgtcagc gagcatgaac acgacgcggt catcatcggc gcgctgcgct
      541 cgcggcattc gctgacgatg cccccgcccg aaccgaatgc tgccgcgcac gcggcctata
      601 tcgcggaggc cagccatatg ctggagcggt tgcgcgagcg gaccgccgcg ccgatcctga
      661 tcgacaacct gcccgagccc acggtgcagc cgctcggtct cgccgaacgc ggattgagcg
      721 gacaccgcaa ccgcttccgt ctggccaatt tggtgctatt cgacctggtg gaatcttata
      781 ccgacgttca tgtcgttgac gtcgccgccg cgctcggatc ggccgggtcc gagacgatgc
      841 tggacgacgg gcaggttggc ttcacgcata tgggctcgcc cggatggctg ctgcagcgtc
      901 ccgacagcga aaaggcggcg gtgcacggca tctttcccga ttcggcgccg cttgcgcgcg
      961 ccgttagcgg ggatccttat agccgcgagg cccttatggc gcgtgcccat gccgatgcgc
     1021 tcacgactgt gctcggtcat gaccgcaaga aatgcgtcat cgtcgatctc gacggcattc
     1081 tatggcccgg cgtgctggcc gagactggca gtcccttcgc ctgggatccg gccattagcg
     1141 gaatgttttc gcatgtcggc ctctattttg gtttgcacga agccttgctc tgcctcaagc
     1201 ggcgtggcgt ggtgctggcc tgcgtcagca agaacgacga ggcgacggtt cgggagctgt
     1261 ggagatatcc cgatcactat ccgcgcagcc gcctcttgac gccggacgat ttcgtcacct
     1321 ggcgcgtcaa ctggaacgac aaggtcgaga atatccgctc gatcgccggc gaactcggtt
     1381 ttgcactcga cgccttctta tttatcgacg atcacccggt cgagcgggac cgcgtgcggc
     1441 aacggctacc ggaggtcgag gtctggggcg acgatctctt ctcattgcgc cgcaggctgc
     1501 tcaatgatcc gcgactgcaa ttgccttgca tcacggcgga atctaccgcg cgcacagacc
     1561 tggtcaaagc gcagctcaac cgccggcatc tgcgggccga tagcatgaac gagggcgact
     1621 acgtcgcctc cttgcagatt cagtgccgta tcggccgaga aaccggcaca agcaaactca
     1681 gccgcatatc ggagctgttc cagcggacca cccaattcaa cgctacgggc cgtaaattca
     1741 cggtcacgga attgaacgat ctgatcgagg gtccggacac ctgcgttttt tcgatggagg
     1801 tgtcggaccg gtttggcgac cacggactgg tcggtgtagc agtgatcgag cgcggcgaaa
     1861 ttaccggtct agtactgagt tgccgcgtgc tcgggcttgg tgtcgagcac cagttcgtcc
     1921 gttacatgat cgaaacgctc aaggccgatc ttggggattt atcaggccgc attgtcgaga
     1981 cgccgcgaaa cagtccggtc cggaatatct atcgcgacct tggatttact cagcgaggcg
     2041 acggcctttg gcggcttgaa ctgaaggcgg agccggcaag gaatcttgct gtcgcctgaa
     2101 tacggtccgg agcatctttg cccgcgcgag gtcgctcagg cgatgcgcgg ctggcgcctt
     2161 ggccgccccg gcgcggcatc cgtcctgtac gttgtcggtt attggcgctt agcggacgta
     2221 ccagccgatt aaatcgatgc ccgctttgag gggcaaaacg gacacggcgc tcagcgtttt
     2281 atgagtaccc gtcccagggc agagcgtgcg gctggacagg gccgccaaga cccactagat
     2341 cattggccat cataacggcg tgggaggtga ggcggtcgcc aaccgtcaat atccgcacca
     2401 ctacaggagt gagtaatggc caaaaaagtc actggattcc tcaaattgca ggtgccggca
     2461 ggcgccgcca acccgtcgcc cccgatcggg ccggcgctgg gtcagcgcgg cctgaacatc
     2521 atggagttct gcaaggcgtt caacgcgcag acccagaaga tcgagaagaa tacgcc
//
LOCUS       DASWZT010000028         3050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_87383, whole genome shotgun sequence.
ACCESSION   DASWZT010000028 DASWZT010000000
VERSION     DASWZT010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87383"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /gene="dxr"
                     /locus_tag="VGE92_00810"
     CDS             <1..928
                     /gene="dxr"
                     /locus_tag="VGE92_00810"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEY1312379.1"
                     /translation="VAADNVDVVMAAIVGAAGLQSTLAAVRAAKRVLLANKEALVMAG
                     PILMDEVRRAGAQLIPIDSEHNAIFQCMPAGYLPGDVARGVQRVILTASGGPFRCTDA
                     AQMVHVTPDEACAHPKWKMGRKISVDSATLMNKGLEVIEATLLFGLPESRVEVIVHPQ
                     SVVHSLVEYADGSLLAQLGAPDMRTPIAQAMAWPERFASGVQSLSLAEIGQLGFEQPD
                     HVRFPSLGLARAAARAGGTAPAALNAANEVAVQAFLDRRLNFTAIAAVIDKVMQRLDS
                     SPVKALGDVLDADAAARRLAAALIEPSSGAFA"
     gene            925..2292
                     /gene="rseP"
                     /locus_tag="VGE92_00815"
     CDS             925..2292
                     /gene="rseP"
                     /locus_tag="VGE92_00815"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEY1312380.1"
                     /translation="MKMLFFVAAFLVAIGILVAVHEFGHYWVAKKLGFKVLRFSIGFG
                     KPLLMRVGKDADRTEYCVAAIPLGGYVKLLDEREAEVSAAELPRSFTRRPVLHRIAVL
                     LAGPAMNLLFASLLYAVLAMVGTEMVKPVVGQVRLDSPAAAAGLQRGDQIVRVGERNV
                     EDTEELQIALIRQFTDDGVIRLRVRRDGNERSLTLRVAEDRRAMTEPGKLLPGLGFDL
                     ATWNAATLVHEAPDGSAGARAGLRAGDRLLAVDGQPIANRLEFIDIISGAPGRDISIE
                     VERGGQRLRIVAAVPRIVEQGRPIGRLGITLEEGPQSWPPGLMQIRRSGPLEAGVSGV
                     AKTWEMSSLTVQMLWRILTGQVSAKNISGPISIAEFAGISAYLGVTAFLAFLAIISVS
                     LGVLNLMPVPLLDGGQVVYQVVEAVKGSPLSERAQLFGQQVGIALLVVLMSLAFYNDI
                     SRHLN"
     gene            2336..>3050
                     /locus_tag="VGE92_00820"
     CDS             2336..>3050
                     /locus_tag="VGE92_00820"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEY1312381.1"
                     /translation="MRLELRLACLVAFAIQPWGSAVRAQTTQEPFTVGDIRIEGLQRI
                     SEGTVFNYLPVNIGDHLDGQRIGEAMRALYATGFFRDVELRRDGNTLLVVVVERPSIA
                     KFEIKGNKDIKTEDLQKSLRNVGLATGKTFDRSVLDEVKQYLTDQYFSRGKYAVRVDT
                     KITELPGNKVDVVVDIKEGKRAKIEQINLVGNTKFKEKDVLATLELKTPNWLSWYKQD
                     DRYSRESLQGDLEKLRSYYM"
BASE COUNT          553 a          884 c         1011 g          602 t
ORIGIN      
        1 ggtcgccgcc gacaacgtcg acgtggtgat ggcggcgatc gtgggtgcgg ccggactgca
       61 atcgactctg gcagcagtac gggccgccaa gcgcgtgttg ctcgccaaca aagaggcgtt
      121 ggtgatggcg ggcccgatcc tgatggatga agtacgccgc gccggtgcgc agctgattcc
      181 gatcgacagc gaacacaatg caattttcca atgcatgccg gcgggttacc tgccgggcga
      241 tgtcgcgcgc ggcgtgcagc gcgtgattct cacggcatcc ggcggtccgt tccgctgcac
      301 cgacgccgcg cagatggttc acgtcactcc cgatgaagcg tgcgcccatc cgaagtggaa
      361 aatgggacgc aagatctcgg tcgactccgc cactctgatg aacaaggggc tcgaggtcat
      421 cgaggcaacc ttgctgttcg gcttgccgga gtcgcgcgtg gaggtcatcg tccatccgca
      481 aagcgtggtg cactcgctgg tggagtacgc ggacggctca ttgttggcgc agctgggtgc
      541 acccgacatg cgcaccccga tcgctcaggc gatggcgtgg ccggagcgat ttgcttccgg
      601 tgtacaatcg ctcagcctgg ccgaaatcgg ccagcttggc ttcgaacagc cggatcatgt
      661 gcgattcccg agtctcgggc tcgccagggc ggcggcccga gccggcggca cggcgccggc
      721 tgcgctgaat gcggccaatg aggtcgcggt ccaggcgttt cttgaccggc gcttgaactt
      781 tactgcgatt gccgcggtca ttgacaaagt gatgcagcgc ctcgattcct cccccgtcaa
      841 ggctctgggc gatgttttgg acgccgatgc cgcggcacga cgcttggctg cagccttgat
      901 cgagcctagc tctggagcct tcgcatgaag atgttgtttt tcgtcgccgc ctttttggtc
      961 gcgatcggga tattggtggc ggtccacgaa ttcgggcact actgggttgc caagaaactc
     1021 ggcttcaagg ttttgcgctt cagcatcggc ttcggcaagc cattgctgat gcgggtcggt
     1081 aaggacgcgg accgcaccga gtactgtgta gccgcaatcc ccttgggcgg gtacgtcaag
     1141 ctgctcgatg aacgcgaagc agaggtgagc gctgccgagc tgccgcgctc tttcacgcgc
     1201 cgtccggtgc tgcaccggat tgccgtgctg ctggcagggc ccgccatgaa cctgctgttc
     1261 gcctcgctct tatatgcagt gttggcgatg gtcggcacgg agatggtgaa accggtggtc
     1321 gggcaggtgc ggctcgacag tccggcggcc gcagccgggc tgcagcgtgg cgatcagatc
     1381 gtgcgcgtcg gcgaacgcaa cgtcgaggac accgaagagc tgcagatcgc gctgattcgc
     1441 cagttcaccg acgacggcgt gatccgtctg cgcgtgcgcc gcgacggcaa cgagcgctca
     1501 ttgacgctgc gggttgccga ggaccggcgc gccatgacgg agcccggcaa attgctgccc
     1561 gggctcggct tcgaccttgc gacctggaac gccgctaccc tggtccacga agcgcctgat
     1621 ggcagcgccg gcgcgcgggc gggcttgcgc gccggcgatc gcctgctggc ggtggacggg
     1681 cagcccatcg ccaatcggct ggagttcatc gacatcatca gcggcgcgcc ggggcgcgac
     1741 atttcgatcg aagtcgagcg cggcggccag cgattgcgta tcgtagcggc cgtgccgcgg
     1801 atcgtcgagc agggcaggcc gatcggccgc ctcggtatta cgctcgaaga gggaccgcag
     1861 agctggcccc cgggactgat gcagatccgt cgttccgggc cgctcgaggc cggcgtcagc
     1921 ggcgtggcga agacttggga gatgtcgtcg ctaacggtgc agatgctgtg gcgtatcttg
     1981 accggtcagg tttccgccaa aaacatcagc ggtccgatca gcatcgcgga atttgccggt
     2041 atcagcgcct atttgggagt gaccgcgttt ttggcgttcc tggcaatcat cagcgtgagt
     2101 ttaggcgtgc tgaatttgat gccggtgccg ttgttggatg gcggtcaggt ggtctatcaa
     2161 gtcgtggaag ctgtcaaggg ctcgcccctt tcggagcgtg cccagctgtt cggtcagcaa
     2221 gtggggattg cgctattggt cgtgttgatg agcttggctt tttataacga tatctcaaga
     2281 catttgaatt aacgggaaat catgatcaat agaaattgca ttcggtcgtt gtcgcgtgcg
     2341 gcttgaattg cgtttggctt gtctggtggc ctttgccatc cagccatggg gatcggctgt
     2401 gcgtgcgcaa accacccagg agccgttcac ggtcggcgac atccgcatcg agggtctgca
     2461 gcgcatctcc gaaggcacgg tattcaatta tctgccggtg aacatcggcg atcatctcga
     2521 tggtcagcgc atcggcgagg ccatgcgggc gctgtacgcg accggatttt ttcgcgatgt
     2581 ggaactgcgc cgcgacggca atacgctgct ggtcgtggtg gtggaacggc cctcgatcgc
     2641 caaattcgaa atcaagggca acaaggacat caagaccgag gacttgcaga agagcttgcg
     2701 caacgtcggc ctcgcgaccg gcaagacgtt cgatcgttcg gtgctcgatg aagtcaagca
     2761 atacctcacc gaccagtatt tcagccgcgg caagtacgcc gtgcgcgtcg acacgaagat
     2821 cacggagctg cccggcaaca aagtcgatgt ggtcgtcgat atcaaggaag gcaaacgcgc
     2881 caagatcgaa cagatcaatt tggtcggcaa caccaaattc aaggaaaaag acgtgctcgc
     2941 caccctcgag ctcaagacgc cgaactggct gtcttggtac aagcaagacg accgctactc
     3001 gcgcgagtcg ctgcagggcg acttggagaa actgcgctcc tactacatgg
//
LOCUS       DASWZT010000029         2096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_326, whole genome shotgun sequence.
ACCESSION   DASWZT010000029 DASWZT010000000
VERSION     DASWZT010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..986)
                     /locus_tag="VGE92_00825"
     CDS             complement(147..986)
                     /locus_tag="VGE92_00825"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY1312382.1"
                     /translation="MPTGGGVGAEIRGLDVRRLDDLNFRLVHRAWIDHQVLLFRGQQL
                     GDAELIAFSRRFGSLDVAPNQETGQRFVAGYPEIYVVSNVVENGRAIGSLGSGEAVWH
                     TDMSYQVDPPKASVLYALEVPAGGGDTGFVNMYAAYEVLPEALKARLAGLRVKHDGTY
                     NSGGYVRYGVTATDDPVAAPGALHPLVCTHPESARRTLYLGRRRNAYIDGLPLAESEA
                     LLDEIWAYATRGEFTWYNHWKLGDLVMWDNRCVMHRREAFDATCRRIMHRTQIKAESR
                     PAA"
     gene            1181..>2096
                     /locus_tag="VGE92_00830"
     CDS             1181..>2096
                     /locus_tag="VGE92_00830"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY1312383.1"
                     /translation="MHETITSYVGLDIHKDSVAIAIAPAGRGAPQFIGTTASNLAQVC
                     KILTRQKCVRSTTLVVYEAGPCGYGWARYLNAQGWCCEVISPAHIAHSAASKAIKTDR
                     RDALLLARESRAGNLTQIVLPDSRDEAMRDLSRAREDAIAARLRARQQLKSMMLRHGR
                     SYHSNTWTPAHERELAKVRFDHPAQQIAFDEYRQMVTEAHERLERITQALREQCAEWR
                     MRPVVEALMCLRGFDFVAAITIVAELGDLTRFAHPRALMAYLGLVPSEHSSGNTRRQG
                     AITRTGNRHARRILVESAWTYRFAAHVGR"
BASE COUNT          382 a          647 c          673 g          394 t
ORIGIN      
        1 ttgccgagcc gtgaggcgac gcgcgccgag ccggtcgcga tgctgacccg atcgatgtag
       61 agcgtcagat acatgaggca gatgatgccc agcacctgat tcggaacgct cagccgcaac
      121 cgcgtgctcg cattttgctc agaggatcac gccgcaggcc tcgattccgc cttgatctgc
      181 gtgcgatgca tgatccgtcg gcaggtcgcg tcgaacgctt cgcgccgatg catgacgcag
      241 cgattgtccc acatcaccag gtcgcccagc ttccaatgat tgtaccaagt gaattcgccg
      301 cgggtcgcgt aggcccagat ctcatccaga agcgcctcgg actctgcgag cggcagcccg
      361 tcgatatagg cattgcggcg gcgccccaga taaagcgtgc gacgcgcgga ttccggatgc
      421 gtacagacga gcggatgcag cgcgcccggc gctgcgaccg gatcgtcggt tgcggtaacg
      481 ccgtaacgca catagccgcc gctgttgtaa gttccgtcat gcttgacgcg aagcccggcg
      541 agccgcgctt tgagcgcctc gggcagaacc tcgtatgccg cgtacatgtt gacgaagccg
      601 gtgtcgccac cgcccgccgg cacttcgagc gcgtacaaca cgctcgcctt gggtggatct
      661 acctgatagg acatatcggt gtgccaaacc gcctcgcccg agccgaggct accgatggca
      721 cggccgtttt cgacaacgtt cgacacgacg tagatctcgg gatagcccgc gacgaagcgt
      781 tggcctgtct cctggttcgg ggcgacatcc aggctgccga aacgtcggct gaaggcaatc
      841 aactccgcgt cgcccagctg ctggccgcga aacagcagca cttggtgatc gatccatgcg
      901 cgatgcacga gacggaagtt cagatcgtcg agccgcctca catcgagccc gcggatctcg
      961 gcgccgacgc cgccaccggt cggcacgatc tcgaacgatc ggcacggcgc gtttgtcaga
     1021 cttgatgtcc ggtcgcgcgt agcattggcg cccgggggat attgctgtgg ccagaggaag
     1081 gggtctgtct cccatatcgt gggttgcgta gttgcgtacg ccgagccaca tgcagaggcc
     1141 ccgaagggat cgtactgctt aacctagcgg gagacagacc atgcacgaga ctatcacttc
     1201 ctatgttgga ttggatattc acaaggactc cgtggctatt gcgattgccc cagcggggcg
     1261 tggtgcaccg cagttcatcg gcaccaccgc cagcaacctg gcccaggtgt gtaagatttt
     1321 gacgcgccag aagtgcgtgc gcagcaccac gttggtagtg tacgaggctg ggccctgcgg
     1381 ttatggctgg gcgcgctact tgaacgctca aggctggtgc tgcgaggtga tttcgccggc
     1441 gcatattgcg cattccgccg cgagcaaggc gatcaagacg gatcggcgtg atgcgttgct
     1501 gctggcgcgt gagtcgcgcg cgggtaactt gacgcagatt gtcttgccgg acagccgcga
     1561 tgaggcgatg cgggatctgt ccagagctcg agaagatgcg attgctgcgc gtcttcgggc
     1621 ccgacagcaa ctgaagtcca tgatgctgcg gcatggccgc tcctatcaca gcaacacatg
     1681 gacgccggcg cacgagcgag agctggcgaa ggtgcgcttt gatcatcccg cccagcagat
     1741 cgccttcgat gagtatcgcc agatggtaac cgaagcgcat gagcgtctgg agcggataac
     1801 ccaggccttg cgcgagcagt gtgccgagtg gcgcatgcga cctgtggtcg aggcgctgat
     1861 gtgcctgcgt ggctttgact tcgtcgctgc gatcacgatc gtcgcagagc tcggcgatct
     1921 gacccgcttc gctcatccgc gtgcgctcat ggcttatctg ggcttggtac cctcggagca
     1981 ctcgagcggt aacacgcgcc ggcaaggcgc catcactcgc actggtaacc gccacgcgcg
     2041 tcgcatcctg gtcgagtcgg cctggactta ccgctttgcg gctcacgtcg gacgcg
//
LOCUS       DASWZT010000030         5760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_87403, whole genome shotgun sequence.
ACCESSION   DASWZT010000030 DASWZT010000000
VERSION     DASWZT010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5760
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87403"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1495
                     /locus_tag="VGE92_00835"
     CDS             <1..1495
                     /locus_tag="VGE92_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005247267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY1312384.1"
                     /translation="PYPPLSELAYSPGVGVDYYLVALQISGVGTLMAGINMVTTILKM
                     RAPGMSYMRMPIFCWTSLASNLLIVAAFPILTAVLAMLMLDRYLGFHFFTNEAGGNPM
                     MFMNLIWAWGHPEVYILILPAFGVFSEVVATFCGKPLFGYRSMVAATMAICIISFMVW
                     LHHFFTMGAGGDVNAIFSIMSMIIAVPTGVKIFNWLFTMYGGRIRYSASMLWAVGFMV
                     TFVIGGMTGVLMAAAPADFVLHNSLFLVAHFHNVIIGGVLFGAFAGYSYWFPKAFGFS
                     LDERLGKAAFWCWLIGFYVAFMPLYAAGLMGMTRRMQHYDRPEWQPLLLIAAAGALVI
                     LCGIGLQVAQLAVSIRTRKQRRDPGGDLWNARTLEWSTASPPPAFNFALLPEVAALDC
                     YWHMKQAARESGTSEATHPGYTPIEMPKNSPTGFITAFFAVVTGFSVIWHIWWLVILG
                     LVGAYMTFVVFAWRDVSETTVPAAEVARIDRAHRLARAQSPQPGFAS"
     gene            1492..2112
                     /gene="cyoC"
                     /locus_tag="VGE92_00840"
     CDS             1492..2112
                     /gene="cyoC"
                     /locus_tag="VGE92_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HEY1312385.1"
                     /translation="MNTDALHGREGTAGSARGPASKRIIVGYGFWIFLLSDVILFSAL
                     FAAYAVLSTATADGPPGRQIFSLSNVAIETGCLLASSFACGMASLAVGQRNARWTQIA
                     LLVTGLLGFAFLTLEFREFAGLIARGDGPRRSAFLSSFFTLVGCHGLHVTAGLLWLGT
                     MMAQFLAKGFRQDIRYRFLCFSLFWHALDIIWVAIFSLVYLVGVAT"
     gene            2109..2513
                     /gene="cyoD"
                     /locus_tag="VGE92_00845"
     CDS             2109..2513
                     /gene="cyoD"
                     /locus_tag="VGE92_00845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HEY1312386.1"
                     /translation="MNSQAVHDDSAGSSFDSAPGDEEDRGSVVRGLVGYLLGLALATL
                     LTAASFWVVSTHLIYGPGVPVAILVLAIAQMGIHLVFFLHITTAADNTNNILALAFGT
                     LIVCLVVFGSLWIMANLDRNMMPMDQLIQMQR"
     gene            complement(2553..3434)
                     /locus_tag="VGE92_00850"
     CDS             complement(2553..3434)
                     /locus_tag="VGE92_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312387.1"
                     /translation="MNYTQDTPPVAQSRSAASWPAIIAGAFVAAAVSLVLFALGSGLG
                     FAAVSPWPSSGISGMSLAVTAAIWLIVTQWLSAAVGGYIAGRLRTRWIGTHTHEIFFR
                     DTAHGLITWAVATIFVAALLAASAFSSAGAAGHAASEMAQAGMAAAGRGMRPAPMAAP
                     PSGVSPADSYEIDRLLRPANAGASAQAGPDLKAEVSRIIANAALAGGMPDGDRAYLAV
                     LVAARTGVSQAEAQKRVDDFVSSVQNAATKAKEAADTARKAAAQAAIYLALSLLVGAF
                     IASVSAAIGGRLRDEHP"
     gene            complement(3554..3949)
                     /locus_tag="VGE92_00855"
     CDS             complement(3554..3949)
                     /locus_tag="VGE92_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEY1312388.1"
                     /translation="MVRPRSFAELFLPHASLRRSKRAAGANENPAIQALWDVVSSIPH
                     GNVATYGAVARAAGLPGRARLAGYALRMSPPGMNLPWHRVLGAGGRIVFPKTSSLYRE
                     QARRLRAEGVAVKEGRVNRTSIMEFDDAP"
     gene            4007..>5760
                     /locus_tag="VGE92_00860"
     CDS             4007..>5760
                     /locus_tag="VGE92_00860"
                     /inference="COORDINATES: protein motif:HMM:NF015089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HEY1312389.1"
                     /translation="MKTIRGFILQTSYRMHSRAGGTRVPVVHVYGRLEQGGTFLLRDD
                     RQRPHFFVRTGDAARVQALAAIEPRPTVKRSFDGAPVSAIEVDAPSEVPAIRDRLHSA
                     GIDTFEADVRFAVRYLIDRGIKGGCEIEGEAIAGGGVSWVFDNPTLRPAEVRLTPEVL
                     SFDIETCGTPQRLLAISMYAAAIDEVLIVDGSDRAMPDHAVRCGDEYSALDAFCERVR
                     RLDPDVLTGWNIIDFDLSVLQQIAARVGHPLPLGRDSGALRIRKAEGYFGSGQANVPG
                     RLVLDGIDLLRGAFIRMDDYSLDAVAREVLGEGKALSGDAQDRVAEINRNYRHDLAAF
                     ARYARTDARLAFDIVRKLKVIQLAFARSQLAGMTPDRVAASIASFDFLYLTELERRDI
                     VAPTVRTSLAGEDAAQQGGHVLEPVAGLHSHVWVFDFKSLYPSIIRTFNIDPISYVAD
                     PAPGTDLIRTPGGAFERQPAILPGVLDVLFPRREAAKKIGDQVASNAIKILMNSFYGV
                     LGTPACRFYNPALANSITGMGREILLWSKRWFESAGFKVIYGDTDSLFVQSRAANPQA
                     ANEQGSQLAADLNADLDR"
BASE COUNT          981 a         1770 c         1795 g         1214 t
ORIGIN      
        1 gccatacccg ccgctgtccg agctcgcgta ttccccgggt gtgggcgtgg attattacct
       61 ggtggcattg cagatttccg gtgtcggtac gctgatggcc ggtatcaaca tggtcaccac
      121 gatactcaag atgcgggcgc ccgggatgtc ttacatgcgc atgccgattt tctgctggac
      181 ctcgttggca tcgaatctgc tgatcgtagc cgcgttcccg attctcaccg cagtgctcgc
      241 gatgctgatg ctcgaccgct atctcggttt tcacttcttc accaacgagg ccggcggtaa
      301 tccgatgatg ttcatgaatc tgatctgggc gtggggacat ccggaggtct acattctgat
      361 tctgcccgcg ttcggtgttt tttcggaggt tgtcgcgact ttttgcggca aaccgctttt
      421 cggataccgc tcgatggtcg ccgcgaccat ggcgatctgc atcatttcct tcatggtttg
      481 gttgcatcac ttcttcacga tgggcgcggg cggcgacgtg aacgccatct tcagcatcat
      541 gtcgatgatt atcgcggtac ccaccggcgt gaagatcttc aactggttgt tcacgatgta
      601 cggcggccgg atccgctaca gcgcgagcat gctgtgggcg gtcggcttca tggtgacatt
      661 cgtgatcggc ggcatgaccg gtgtgctcat ggccgcagcg cccgccgact tcgtgttgca
      721 taacagcctg ttcctggtcg cccactttca caatgtcatc atcggaggag tgctgttcgg
      781 tgctttcgcc ggttacagct actggtttcc caaggcattc ggattcagtc tcgatgaacg
      841 cctcggtaaa gcggcattct ggtgctggct gatcggcttc tatgttgcct tcatgccgct
      901 gtacgcggca ggtctgatgg gaatgacccg gcgcatgcag cactatgacc gacccgagtg
      961 gcagcccttg ctgctgatcg cggccgccgg tgcgctggta attctgtgcg gtatcggctt
     1021 gcaggtggcg cagctcgccg tctcgatccg cacgcgcaag cagcggcgag atccgggcgg
     1081 cgacttatgg aatgcgcgca ccttggaatg gtcgaccgcc tcgccgccgc cggcattcaa
     1141 cttcgcgctc ctgccggaag ttgccgcact cgattgctat tggcacatga aacaagcagc
     1201 gcgcgagtcc ggcacgtccg aggccacgca ccccggctat acgccgatcg aaatgcccaa
     1261 aaactcgccg accgggttca tcacggcatt ttttgcggtc gtcaccggct tttcggtgat
     1321 ttggcatatc tggtggttgg tgatcctcgg cttggtgggc gcctacatga cgtttgtcgt
     1381 gttcgcgtgg cgtgacgtca gcgagaccac cgtgccggcc gccgaagtgg ctcgaatcga
     1441 ccgcgctcat cggctggcgc gcgcgcaatc gccgcaacct ggatttgcat cgtgaacacg
     1501 gacgcgctgc acggccgaga gggcaccgcc ggcagtgctc gtggtcccgc ctcgaagcgg
     1561 atcatcgtcg ggtatggctt ttggattttt ctgttgtccg acgtcatcct gttttccgca
     1621 ttgttcgcag cctacgcggt gctttcgact gccacggccg acgggccgcc aggccgacaa
     1681 attttttcgt tatccaatgt cgccattgag accggatgct tgctcgcatc gagcttcgcg
     1741 tgcgggatgg cctcgctcgc cgtcggtcag cgcaatgcgc gctggacgca aatcgccttg
     1801 ctggtgaccg ggctgctcgg attcgcgttt ctcaccctcg aatttcgcga atttgccggg
     1861 ctgatcgcgc gcggcgacgg tccgcggcgc agtgcattcc tctcgtcgtt ctttacgctg
     1921 gtcggctgcc atggcttaca cgtgacggcg gggctgctgt ggcttggaac gatgatggcg
     1981 caatttctgg cgaaaggatt tcggcaggac atccgctatc gatttctgtg cttcagtttg
     2041 ttttggcacg cgctcgacat catttgggtg gcaatcttca gcttggttta cctggttgga
     2101 gttgcgacat gaattcacag gctgttcatg acgactcagc cggcagttcg ttcgacagtg
     2161 cgcccgggga tgaagaggac cgcggcagtg tggtgcgcgg cctggtcggc tatctgctgg
     2221 ggttggcgct tgccacactg ctcaccgcgg catccttctg ggtggtgtcg actcacctca
     2281 tctacgggcc gggagtcccg gtggccattt tggtgctcgc cattgcgcaa atgggaatac
     2341 acctcgtgtt ctttctgcac attacaaccg cggcagataa caccaacaac attctggcgt
     2401 tggcctttgg cacgctcatc gtctgcctgg tcgtgtttgg gtcgctgtgg atcatggcga
     2461 acctggatcg caacatgatg cccatggacc aactcattca gatgcagcgg tgaactgtga
     2521 attcgcgcgc tctcgactcg agggcggcgt gactaagggt gttcatcgcg caagcgaccg
     2581 ccgatggctg cggacacgct ggcgatgaag gcgccgacga gcagcgacaa tgccaggtaa
     2641 atcgccgctt gagccgcagc tttgcgcgcg gtatcggctg cttccttcgc tttggttgcg
     2701 gcattttgaa cgctcgacac gaagtcatcg acgcgctttt gcgcctctgc ttgcgaaacg
     2761 ccggttcgag cggcaaccag aacagccagg taggcacgat cgccatccgg catcccgccc
     2821 gcgagggcgg cattcgcaat gatgcggctc acttcagctt tgaggtccgg tcccgcttgg
     2881 gcgctcgcac ccgcattggc ggggcgcagc aagcgatcga tctcatacga atcagcgggg
     2941 gaaacgccgc tcggcggtgc cgccattggc gccggcctca tgcccctccc ggccgccgcc
     3001 atcccagcct gggccatctc ggaggcggcg tgtccggccg ctccggccga tgagaacgcg
     3061 ctcgccgcca acagggcagc cacgaaaatc gtcgccactg cccaggtgat caagccgtgt
     3121 gcggtatctc tgaagaagat ctcgtgtgtg tgcgttccaa tccatcgcgt acgtaagcgg
     3181 cccgcgatgt agccgccgac ggcagccgac aaccactgcg tgacaatcag ccaaatggcg
     3241 gctgtcacgg ccagggacat accggaaatc ccgctgctgg gccagggcga aactgccgcg
     3301 aatccgagcc ctgaaccaag cgcaaacaac accagtgaaa ctgccgcggc aacaaaagcc
     3361 ccggcgatga tcgcgggcca ggacgccgcg gatcttgact gcgcgaccgg cggtgtgtct
     3421 tgggtgtaat tcacgagatt cttcccattt cttgcttggc tgagggctag tgtcgaccgg
     3481 gaaaactcta ggcgtgaatg ctctcgtgag ccatcgtctg ccggttcaat tcgctgtaag
     3541 gcctatccta cggtcaggga gcgtcgtcga actccatgat ggaggtgcgg ttgacgcggc
     3601 cctccttgac agcgacccct tcagcccgca agcgccgggc ttgttcgcgg tacagcgatg
     3661 acgtcttcgg aaacacgatg cggccgccgg cgccgagcac gcggtgccac ggcagattca
     3721 tgccgggagg cgacattcgc aaggcgtatc ccgcgaggcg cgcgcggccg gggaggcccg
     3781 cggctctggc gaccgctcca taggtggcga cattgccgtg cgggatcgag ctcacgacat
     3841 cccacagcgc ctgaatggcg gggttttcat tggcgccggc ggctcttttc gaccggcgca
     3901 gactcgcgtg cggcaggaat aattcggcaa atgatcttgg gcgcaccatt cgccgatcat
     3961 acttgcgctg ctgtcgcaac ccgcatcgaa cgcgcaaaat agtcgggtga aaacaatccg
     4021 tggattcatc ctgcagacca gttaccgcat gcactcccgg gccggcggca cgcgtgttcc
     4081 cgtggtgcac gtctatggac ggttggagca gggcggcacc ttcctcttgc gtgatgatcg
     4141 tcagcgcccg cattttttcg tgcgaacggg cgatgccgcc cgggtgcagg cactcgcggc
     4201 gatcgaacct cggcccaccg tcaagcgttc gttcgacggt gcgccggtgt ccgcgatcga
     4261 agtcgacgcc ccatccgagg tgcctgccat cagggatcgg ttgcacagcg ccggcatcga
     4321 taccttcgag gcggatgtgc gtttcgccgt tcggtacttg atcgatcgcg gcatcaaggg
     4381 cggctgcgag attgagggcg aggcgattgc ggggggcggc gtttcgtggg ttttcgataa
     4441 cccgacgctg cgccccgcgg aagtgagact gacccccgaa gtgttgtcgt tcgacatcga
     4501 aacgtgcggc acgccgcagc gactgctcgc catctcgatg tatgcggccg ctatcgatga
     4561 ggtgctcatc gtcgacggca gcgatcgcgc catgccggat cacgcggtgc gctgcggcga
     4621 cgagtactcg gcgctcgacg cattttgtga gcgcgtgcgg cggctggatc cggatgttct
     4681 gaccggttgg aatatcatcg atttcgatct cagcgttttg cagcagatcg ccgcccgagt
     4741 aggtcacccg ctgcccttgg ggcgcgactc gggcgcgctg cgaatccgca aggccgaggg
     4801 ttatttcggc agcggccagg cgaatgttcc cggccgcctg gtgctcgacg gcatcgatct
     4861 gctgcgcggt gcgttcatcc gcatggacga ttactcgctc gatgccgtgg cacgcgaagt
     4921 gctgggcgag ggcaaggcac tgtcgggcga cgcgcaagat cgcgtggccg agatcaaccg
     4981 caactatcgg cacgacctgg cagcgtttgc acgctacgca cgcaccgacg ccaggctcgc
     5041 gttcgacatc gtgcgcaagc tcaaagtgat acaactcgcg ttcgcccgca gccaactcgc
     5101 cggcatgacg ccggaccgcg ttgcggcgag cattgcctcg ttcgattttc tctacctcac
     5161 cgaactcgag cggcgggaca ttgttgcgcc gaccgtgcgc acgtcgctcg caggcgaaga
     5221 tgccgcccaa cagggcggcc acgttctcga acccgtcgcc ggcctgcata gccatgtctg
     5281 ggtgttcgat ttcaaaagtc tatacccgag catcattcgc acattcaaca tcgatccaat
     5341 atcgtacgtc gcggatccgg cacccggaac cgacctgatt cgcacgccgg gcggtgcgtt
     5401 cgagcggcaa cccgcgattt tgccgggggt gctcgatgtg ctgtttccgc ggcgcgaggc
     5461 ggccaagaaa atcggcgatc aggtggcgtc caatgcaatc aagatattga tgaactcctt
     5521 ctacggggtt ctcggcacgc cggcgtgccg cttctacaat cccgcattgg ccaactcgat
     5581 caccggcatg ggccgagaaa tactgctgtg gtcgaaacgc tggttcgaga gcgccggatt
     5641 caaggtcatc tacggcgaca ccgacagcct gttcgttcaa tcccgggccg cgaatccgca
     5701 ggccgccaac gagcaaggca gccaattggc cgcggaccta aacgccgacc tcgaccgcca
//
LOCUS       DASWZT010000031         1533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_19688, whole genome shotgun sequence.
ACCESSION   DASWZT010000031 DASWZT010000000
VERSION     DASWZT010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19688"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VGE92_00865"
     CDS             <1..864
                     /locus_tag="VGE92_00865"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY1312390.1"
                     /translation="PFDFRMGYGKRISKELTLVQIDMDYRTVGKNRDISLGLVGDPGA
                     ILGAVLQAASGRLKNDKRQARQQWLKQLSDAENVAAEKLLPLFKSNSVPIHPYRVAWE
                     LNEFLRDDTIYIGDGGDVVTISAQAVRPRNPGQWMDPGALGSLGVGTGFAIAAGLANP
                     AKEVLCYYGDGSFGMTAFDMETANRFGVPYLAVIGNNSAMNQIRYGQLAKYGEQRGSV
                     GNLLGDVPFGKFAEMLGGYGEEVRDPAQIAPALQRARESIRKSRRSAVVNIWVDPREY
                     APGTKNQTMYK"
     gene            880..>1533
                     /locus_tag="VGE92_00870"
     CDS             880..>1533
                     /locus_tag="VGE92_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY1312391.1"
                     /translation="MSKALDGVRILDFTHVQSGPTCTQLLAWFGADVIKVEKAGSGDV
                     TREQLRDVPDADSLYFTMLNHNKRSVTLDTKSDSGKQVLEKLVKHCDVLVENFAPGAL
                     DRMGFTWERIQELNPRMIVASVKGFGPGPYEDCKVYENVAQCAGGAASTTGFDDGPPL
                     VTGAQIGDSGTGLHLALGIVAALFQRNTSGRGQKVLAAMQDGVLNLCRVKLRDQQRLA
                     "
BASE COUNT          296 a          462 c          518 g          257 t
ORIGIN      
        1 ccgttcgact tccgcatggg ctacggcaag cgcatcagca aggagctgac gctggtgcag
       61 atcgacatgg attatcggac cgtcggcaag aatcgcgata tcagtctcgg gctggtgggc
      121 gatccgggcg cgattttggg agcggtgctg caagcggcaa gcggccgcct caagaacgac
      181 aagcggcaag cgcggcaaca gtggctcaag cagctgagcg atgccgaaaa cgtcgccgcc
      241 gagaagctct tgccgctgtt caaatccaac agcgtgccga ttcatccgta ccgcgtcgcc
      301 tgggagctca acgaattcct gcgcgacgac acgatctaca tcggcgacgg cggcgacgtg
      361 gtgaccattt cggcgcaggc cgtgcggccg cgaaatccgg gacagtggat ggatccgggt
      421 gcgctcggtt cgctcggcgt cggcaccgga ttcgcaatcg ccgccgggct tgcgaacccc
      481 gccaaggagg tactgtgcta ctacggcgac ggctcgttcg gtatgacggc cttcgatatg
      541 gagaccgcga accgcttcgg cgtgccgtac cttgcggtca tcggcaataa ctcggccatg
      601 aaccagatcc gctacggcca gctggcgaag tacggcgagc agcgcggcag cgtcggcaat
      661 ctgctgggcg atgtgccgtt cggcaagttc gcggagatgc tcggcggtta cggtgaagaa
      721 gtgcgcgacc ctgcgcagat cgccccggcc ctgcagcgcg cgcgcgagtc gatccgcaag
      781 tcgcgacgtt cggcggtggt gaacatctgg gtcgatccgc gcgagtacgc gccgggcacc
      841 aaaaaccaaa cgatgtacaa gtgaggcctg gagcgttgca tgagtaaggc attggacggc
      901 gtccgcattc tagacttcac ccatgtgcaa tcgggtccga cctgcacgca gctgttggcc
      961 tggttcggcg ccgatgtgat caaggtcgag aaggccggca gcggcgacgt cacgcgcgaa
     1021 caactgcgcg atgtgccgga tgcggacagc ctgtacttca ccatgctgaa tcacaacaag
     1081 cgctcggtga cgctcgacac gaaaagcgac agcggcaagc aagtgctcga gaagctggtg
     1141 aagcattgcg acgtgctggt cgagaacttt gctccgggcg ctctcgatcg catggggttc
     1201 acctgggagc gcattcagga actcaatccg cgcatgatcg tcgcctcggt gaagggcttc
     1261 ggccccgggc cctatgagga ctgcaaggtc tacgagaatg tcgcccaatg tgccggcggc
     1321 gccgcgtcga ccacgggctt cgatgacgga ccgccgttgg tcaccggcgc gcaaatcggc
     1381 gacagcggca ccgggctgca cctggcactc ggcatcgtcg cggcgttgtt ccagcggaac
     1441 acctcgggac gcggccagaa ggtgttggcg gccatgcagg atggggtgct caatttatgc
     1501 cgcgtcaagc tgcgcgatca gcagcggctc gcg
//
LOCUS       DASWZT010000032         3200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_39065, whole genome shotgun sequence.
ACCESSION   DASWZT010000032 DASWZT010000000
VERSION     DASWZT010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39065"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VGE92_00875"
     CDS             <1..687
                     /locus_tag="VGE92_00875"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY1312392.1"
                     /translation="GALDLELEPAAVATYAKALPIGDTAELLRRRPDVRAAERQLAAA
                     TARVGVATADLFPRVNVTGFIGFLSGDVGRLFSTTSDNNARAWSIAPTVSWAAFDMGS
                     VRARLHASEAQSDAAAANYEKVVLTALEDTENSFLAYSARQTQLKSLSEQAQASRRAA
                     ELADTQYREGVADFLVLLDAQRTQLDAEDSVAQAETAVNVSVVAIYKALGGVGQPLND
                     AAPIKVARIP"
     gene            721..1965
                     /locus_tag="VGE92_00880"
     CDS             721..1965
                     /locus_tag="VGE92_00880"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY1312393.1"
                     /translation="MCNKAWTMQLRIPIAILLGVTLLLQACAQTATRAPTSTQGEAPN
                     AIRTLGPDQRFSTLSSQSVARRVRAERAGQPVAILALSGGGADGAFGAGALVGLTRSG
                     LRPQFSVVTGVSTGALIAPYAFLGSDWDDELVEVYTSGRAEHLLHARGLGALFGSSIY
                     SGTPLKELVDRYATDALVKAVAREAATGRLLLVATTDVNTGEPVIWDLGSIAMNGGAG
                     ARALFRDVLVASASVPGLFPPVVIRVQEQQALYEEVHVDGAIAVPFFVPLGFIASTRD
                     TNDPSRGTAIYVIVDGRLGEPPAQIRLRTQSILSRSVSAGLDHMMRTTLELTATDAKL
                     QGAEFQFAAIPVAYPTLHAFDFGTPTMRSLFQFGYSCAQAGRLWSSSRSVAGVETQHD
                     AGTGSHNQCPADDEFIGRLAVR"
     gene            2205..2981
                     /locus_tag="VGE92_00885"
     CDS             2205..2981
                     /locus_tag="VGE92_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY1312394.1"
                     /translation="MSSEKSPATVLITGATGGIGTALAHLYAQPQRTLILHGRDATRL
                     AALARDCEARGARVVSTSFDLRNGEATVATLRQLSGTHRIDLAIVNAGVSSTIGQGEE
                     VEQWETARAVLAVNLDGALATVAGVLPEMRRRGAGQIALVSSLEAYIGMPVTPVYSAS
                     KAAVKAYGEALRGWLAPQGVHVNVVLPGFVRTPMSDHYPAAKPWIMSPERAAHMIRRG
                     LERNRARTAFPRVLAWGMWWLSVLPPSLSQWILRALGYGG"
     gene            2978..>3200
                     /locus_tag="VGE92_00890"
     CDS             2978..>3200
                     /locus_tag="VGE92_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312395.1"
                     /translation="MSAVQRINDSVLGSLERPALAWLVRRLPRKVVPDHLTAFGVFGA
                     CLTVAGFVLSSRALPWLWLASLGLIVNWLG"
BASE COUNT          543 a         1049 c         1032 g          576 t
ORIGIN      
        1 ggcgcgctcg atctggagct cgagcccgcc gccgtcgcaa cttacgcgaa agctctcccc
       61 attggcgaca cggccgagct tctgcggcgg cggcctgatg tgcgtgccgc cgaacgccag
      121 cttgccgccg ccacggcgcg cgtcggtgtc gcgaccgcgg acttgttccc gcgcgtcaac
      181 gtcaccggtt tcatcggttt tctatcgggg gacgtgggcc ggctgttcag caccacgtcc
      241 gacaacaatg cgcgcgcgtg gtccatcgca cccaccgtga gttgggcggc attcgatatg
      301 ggctcggtgc gcgcgcggct gcacgcaagc gaagcgcaat ccgacgccgc ggcggccaac
      361 tacgagaagg tcgtactgac ggcgctcgag gataccgaaa atagtttcct cgcctacagc
      421 gcacggcaaa cgcaattgaa aagtctgagc gaacaagcgc aagcctcgcg gcgcgcggcg
      481 gaactggcgg atacgcagta ccgggagggc gtcgcggact ttctggtatt gctggacgcg
      541 cagcgcacgc aacttgacgc cgaggactcc gtggcgcagg ccgagacggc cgtgaatgtg
      601 tccgtcgtcg ccatctacaa ggcgctcggc ggcgttgggc agccgctgaa cgatgccgcc
      661 cccatcaaag tggcgcgaat tccgtgaagg gcgcgtccgg tgagacatcg gtgagccagt
      721 atgtgcaata aggcatggac catgcagctg agaattccaa ttgccatcct gctcggcgtc
      781 actctgctcc tgcaagcgtg cgcgcagacc gccacgcgcg cgcccacatc aacccaggga
      841 gaggcgccca acgcaatccg cacgctcggg cccgatcagc gcttctcgac gctgtcctcc
      901 cagagtgtcg cgcggcgcgt tcgagctgag cgcgcggggc agcccgtcgc tatcctggcg
      961 ctctcgggag gcggggccga cggcgccttt ggcgctggtg cgctggtcgg tctcacacgc
     1021 tccgggttgc ggccccaatt ttccgtcgtc accggtgtca gcaccggcgc tttgatcgct
     1081 ccctacgcct tcctgggctc cgactgggac gatgaactcg tcgaggtcta cacatccggc
     1141 cgcgcagagc acctgctgca tgcccgtggg ttgggggcgc tgtttggctc gagcatctat
     1201 agcggcacgc cgctcaagga gctcgttgac cgctacgcca ccgatgccct ggtcaaagcg
     1261 gtcgcacgcg aggcggcgac cggacgactg ctgctcgtgg cgacgaccga tgtcaacacc
     1321 ggagagccgg tgatctggga cttgggatcg atcgccatga acggtggcgc cggcgcacga
     1381 gcgctgtttc gcgatgtctt ggtggcctcc gccagcgtgc cgggactgtt tcccccggtc
     1441 gtcatccgcg tgcaagagca acaggcgctc tacgaagagg tccacgtcga cggcgcaatt
     1501 gccgtcccgt tttttgtgcc gcttggcttc atcgcgtcca cccgagatac aaacgacccg
     1561 tcgcgtggca ctgccattta cgtcatcgtc gatgggcgac tcggcgaacc gcccgcgcaa
     1621 atccggttac ggacccaatc gattctatcg cgcagcgttt ccgcgggcct cgatcacatg
     1681 atgagaacga cacttgaact gaccgccacc gatgcgaaac tgcaaggcgc agagttccaa
     1741 ttcgcagcca ttcccgtcgc ctacccgacg ctgcacgcgt tcgatttcgg aacgccaacc
     1801 atgcgatcgc tgttccaatt cggctatagc tgcgctcaag cgggacgact atggagctca
     1861 tcgcgaagcg tcgcaggtgt cgagacacag catgacgccg gcaccggatc acacaaccaa
     1921 tgtccagccg atgatgaatt catcggccgc ttggccgtgc gctagatgaa aaaacgcaat
     1981 cgcccgctaa agtccctgcc cgtggcgaac tatgaatcgg ccattgcccg cgccgtcaag
     2041 tggttgggcg aacgctatct actcgcgaag cccataaaat ctgcgcctcg ctcgggcgtc
     2101 gatagtgacc ctcgatgagg cagggtcact gcgctgtaga ttattgattt tcattaccgg
     2161 tgcaggctgc gacggtataa gctggcgccg atggcaggcc tctattgagt tccgagaaat
     2221 cgcccgccac ggtgctcatc accggggcga cgggcggcat cggaacggct ctcgcgcatc
     2281 tttatgcgca gccgcagcgc accctgatcc ttcatggccg cgacgccacg cggctcgctg
     2341 ccctcgcccg cgactgcgaa gcccgcggcg cgcgcgtggt gagcacttca ttcgacctgc
     2401 gcaacggcga agccacggtg gcgacgcttc gccaactgtc cggcactcat aggatcgatc
     2461 tcgcgatcgt caacgccggc gtcagcagca cgattggcca gggtgaggaa gtcgaacagt
     2521 gggagacggc acgcgcagtg ctcgcggtta atctcgacgg cgcgctcgcg accgtcgccg
     2581 gcgttcttcc cgagatgcgc cggcgcggcg ccggccagat cgccctggtg agttcgctcg
     2641 aggcatacat cggcatgcca gtgacgccgg tctatagcgc gagcaaggcc gccgtgaagg
     2701 cttacgggga ggctctgcgc ggttggctcg cgccgcaggg cgtccacgtg aacgtcgtat
     2761 tgccgggttt cgtgcgcacg ccgatgagcg atcactaccc tgcagccaag ccttggataa
     2821 tgtcgcccga acgagcggcg catatgattc ggcgcggact cgagcgcaat cgcgcgcgca
     2881 cagcgtttcc gcgcgtactc gcgtggggga tgtggtggct gtcggtcctg ccaccgtcgc
     2941 tgtcgcagtg gatactgcgc gcgctaggat acggcgggtg agcgcggtgc aacggatcaa
     3001 cgatagcgtg ctcggttctt tggagcggcc cgcgctcgcg tggctcgtac ggcgcctacc
     3061 gcgcaaagtc gtgccggatc atctcaccgc gttcggcgtg ttcggcgcct gcctcaccgt
     3121 ggccggcttc gttttgagct cccgagcgct gccctggctg tggctggcga gtctcgggct
     3181 catcgtcaat tggctgggcg
//
LOCUS       DASWZT010000033         3164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_77711, whole genome shotgun sequence.
ACCESSION   DASWZT010000033 DASWZT010000000
VERSION     DASWZT010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77711"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2563)
                     /locus_tag="VGE92_00895"
     CDS             complement(<1..2563)
                     /locus_tag="VGE92_00895"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1312396.1"
                     /translation="MRLRLHRREFALALVLTAAVINAHAKVFVYRGDLKVSATSGTGC
                     PADQTLTIPITLYIRDDAKPDRYEGYIVGALGTSRLSGRRLDDLRGTTPNVDPDLETR
                     AHASIDRVGAEELSGEAHAPSVPAQTPTCYADKVSFRLARSADAHPAEAFTLAQDGFA
                     WLDFSTKKDNASALSAARDALGVAEHTFGLNDIAVRNALNNEAVTLVALGQWTETEPL
                     LRRMVAIDEQAHQNKGPELVQSLASLGSILQASHKYEEAEKVLRRAFATEGQTAEQRA
                     FIAPRLWQIKSASSAEKYREIEIVMRDEVAKEEQAKDQGKLALALFQLGDHLEKTGRY
                     AEAEPLLRRSLDIAEKLLPADDPRLTYVFGDLGTLVNNTGRFSEARRLLERAVAIAEK
                     AQDATQLPIQLEALAFNLQCVGRYSESEVLARRALKLTESAVPRNEADVAKAEAQLAN
                     VLDASHHYAEAETYQRRVVTLSEQGLGAEDPTLGYVLGRLGNTLVAEHKYQEATALIT
                     RSIAICEKGLGPESAALIVPLVDFATMMRDSGRYAEAQAALERAFRLARTLDIAIDWL
                     TAGSLMKFYRDPHVAQPTIAIYYGKQAVNALQGLRGNLGDTGSDQSFVDKMAPIYREL
                     AEVLIQQGRLSEAQQVLAMLKEQELIQFTQGGAVGDVRKTSVEFNAAEKKLAAQDDAL
                     IGLEKQSLPLQAKLRADGELSAADQATLDEMQSKRQLEQRIFQTEVTVAANSPDRQLD
                     SLASQYVALGKEYGALQEKFQKAGELNAADRSRLLELRAAMDSAQATFEARASEVAKS
                     SSDPEAQKRRRQEINDFSRAFEGTLKSMGHDAVLAQFFILDDKVAILLTTPNAVVA"
     gene            complement(2631..3053)
                     /locus_tag="VGE92_00900"
     CDS             complement(2631..3053)
                     /locus_tag="VGE92_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312397.1"
                     /translation="MNKTAAAAAIGIALLLLGTAWTADADRHFDGTWDTILSCVNSNG
                     ALGYSFQFPSTVKEGVLHGEKGTRGQPGWLQIDGKILQDGSANLYAAGLVGAAPFAVG
                     QRPAGTEYGYHIDAKFSDTSGKGARVEGRPCTVTFTKN"
BASE COUNT          544 a          989 c          967 g          664 t
ORIGIN      
        1 gcgccaccac cgcgttgggc gtggtcagga ggatcgcgac cttgtcgtcc aatatgaaga
       61 attgggccaa gacggcatcg tgtcccatcg acttcagggt gccctcgaaa gcccggctga
      121 aatcgttgat ctcttgcctg cgccgcttct gagcttccgg gtcgctcgag ctcttggcga
      181 cttcggacgc tcgcgcttcg aatgttgctt gagcggagtc catggccgca cgcaattcca
      241 gcaagcggga ccggtcggcg gcattcagtt cgccggcttt ttggaacttc tcctgtaacg
      301 cgccgtattc cttgccaagg gccacatact gactcgccaa cgagtcgagc tgtcgatcgg
      361 gcgaattcgc cgcaaccgtg acctcggtct gaaagatcct ctgttcgagt tggcgcttgc
      421 tctgcatctc atcgagcgtg gcctgatcgg cggcgctcag ttctccgtcc gcacgcaatt
      481 tcgcctgcag cggcaggctt tgtttttcca aaccgatcag ggcatcgtct tgtgccgcga
      541 gcttcttctc cgcggcgttg aattcgacgc tggtcttgcg cacatcgccg acggcgccgc
      601 cttgggtgaa ttggatcagc tcctgttcct tgagcatcgc caggacctgc tgcgcttcgc
      661 tcaagcggcc ttgctgtatc agcacttccg cgagctcacg gtagatcggt gccatcttgt
      721 cgacgaagga ctggtctgat ccggtgtcgc ccagattgcc gcgcaaaccc tgcaatgcat
      781 tcacagcctg cttgccgtaa tagattgcga tagtcggttg cgccacgtgc gggtcacggt
      841 aaaacttcat caggctgccg gcggtcagcc aatcgatggc gatgtccaac gtgcgggcga
      901 gccgaaaagc gcgctcgagc gccgcctggg cttccgcata gcggccgcta tcgcgcatca
      961 tggtcgcgaa gtccactaac ggcacaatca acgcagcgct ttccggaccc aagccctttt
     1021 cgcagatcgc gatcgaccgc gtaatcaacg cagtggcctc ctgatatttg tgctccgcga
     1081 ccagggtatt tcccaaacgg ccgagcacgt agccgagcgt cggatcctcg gctcccaagc
     1141 cctgctcgga gagggtgacc acccggcgtt gataggtctc agcctcggcg tagtggtggg
     1201 aggcgtccag cacgttggcc agctgcgctt cggccttggc gacgtcggcc tcgttacgcg
     1261 ggaccgcgct ttcggtcagt ttgagcgccc tgcgtgccag cacctcggat tcgctgtaac
     1321 gtccgacaca ctgcaaattg aaagcgagag cctcgagctg gatcggcaac tgagtcgcgt
     1381 cctgcgcctt ctccgcgatc gcgacggcgc gctcgagaag ccgccgcgct tcggaaaaac
     1441 ggcccgtgtt attgaccagc gttccgagat cgccgaacac gtaggtcaga cgcggatcgt
     1501 cggcagggag cagtttctcc gcgatgtcca atgatcgccg cagcagcggc tcggcctccg
     1561 catagcggcc ggttttctcc aggtggtcgc ccagctgaaa cagcgccagg gcgagctttc
     1621 cttgatcttt tgcctgctcc tccttggcga cctcatcccg catgacgatc tcgatttccc
     1681 gatatttctc cgccgagctc gcgctcttga tctgccacag gcgaggggcg atgaaggcgc
     1741 gctgctccgc tgtctgtccc tccgtggcaa acgcccggcg cagcaccttt tccgcctcct
     1801 cgtacttgtg cgacgcctgc aatatcgagc cgagcgaggc caagctttga acgagttcgg
     1861 gccctttgtt ctgatgtgcc tgctcatcga tggcgaccat gcgtcgcagc agcggctcgg
     1921 tttcggtcca ttggcccaac gccaccagcg tcacggcttc attgttgagt gcgttgcgca
     1981 cggcgatatc gttcaggccg aaggtatgtt ccgccacgcc aagcgcatcg cgggcggccg
     2041 acaacgcgct cgcgttgtcc ttcttggtac tgaagtcgag ccacgcgaat ccatcctggg
     2101 cgagcgtgaa cgcttcggct gggtgcgcgt ccgcgcttcg cgcaagcctg aagcttactt
     2161 tatcggcgta gcaggtcggc gtctgtgccg gtaccgatgg cgcatgggcc tcgccgctca
     2221 actcctcggc gccaacgcgg tcgatggacg catgggcgcg agtctcgagg tcgggatcga
     2281 cattcggagt cgtgccacgc aggtcgtcga ggcgcctacc gctgagtcga ctcgtcccga
     2341 gtgcaccaac gatataaccc tcataacgat cgggcttggc gtcatcgcga atgtacagcg
     2401 tgatggggat cgtcaaggtt tgatccgcag gacatcccgt accggacgtg gccgagacct
     2461 tgaggtcgcc gcgatacacg aagacttttg cgtgggcatt gatgacggcg gcggtcaaca
     2521 cgagggccaa cgcgaattct cgacggtgca gtcgcaaacg catacgacat aaccttggcg
     2581 gcgcgcgggt gacagatcgc gccgcccggg aatcctatcc catcctgagt ctagtttttg
     2641 gtgaaagtaa ccgtgcaggg gcggccttcg acgcgcgctc ctttgccgga ggtgtcggaa
     2701 aacttggcgt cgatgtgata accgtactcg gtgcccgccg gccgttgtcc gacggcgaac
     2761 ggcgcggcgc cgacgagccc ggcggcgtac aggttggcgg aaccatcttg aagaatcttg
     2821 ccatcgatct gcagccagcc gggttgaccg cgggtgcctt tctcgccgtg cagaacgccc
     2881 tctttcacgg tcgatggaaa ttgaaaggaa taacccaagg cgccgttcga attgacgcac
     2941 gagagtatcg tgtcccacgt gccatcgaaa tggcggtcgg cgtcggcggt ccatgccgtg
     3001 cctaagagca gcagagcgat gccaatcgcc gcggcggcgg ccgtcttgtt catgtcgcat
     3061 ttcccttcag gttcgcggcc cgagtgcgtt caagcggtat tataattcga acaccagcag
     3121 tcggcggagc acatcgatgg tgtcggatcg agcgtggttg ttgt
//
LOCUS       DASWZT010000034         1590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_87413, whole genome shotgun sequence.
ACCESSION   DASWZT010000034 DASWZT010000000
VERSION     DASWZT010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87413"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..1103
                     /locus_tag="VGE92_00905"
     CDS             216..1103
                     /locus_tag="VGE92_00905"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002306.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEY1312398.1"
                     /translation="MSHSFQSIALFGNAKDQRVAECMLSLVSHFHSQGLRPLVDPEVG
                     LAFQPDSVLPCPEAAFATRADLIIAVGGDGTLLYAARLVAGHSVPLLGINRGRLGFLT
                     DVSPNSMLEDVDAVLAGRYSEDRRSLLAARLEKSGVESVRALALNDVVVNKWETGRTM
                     DFETSINGRFVNSHGGDGMVIATATGSTAYALSCGGPIVEPNLDVWVLAPISPHTLSD
                     RPIVVRADSKIQLRMSDRFESHAQVTCDGRAIGDLEQGDNLYVEAAEAQITLLHPPGY
                     DYYRLLRSKLHWGRGGYSD"
     gene            1109..>1590
                     /locus_tag="VGE92_00910"
     CDS             1109..>1590
                     /locus_tag="VGE92_00910"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY1312399.1"
                     /translation="MLSHLQLRDFVLVDRAELELKPGLTALTGETGAGKSIVVDALLL
                     IAGGRAAGDVVRQGAERAEVAASFDALPDAAQAWLEAQSIDLDGDLVLRRVIGADGRS
                     RAYVNGQVVPIQALRELAQLLIEIHGQQEFQRLVNRGAQRDMLDDHLADRALLPAVSE
                     L"
BASE COUNT          260 a          520 c          513 g          297 t
ORIGIN      
        1 cgcgtgacag cgtgcgcgag ccgacgggtt ggccgtcgcg aatgtagttt tcgatcaagg
       61 ccttgagcag cagctgggcc cgctcggtga gtccgtcctc ggcaccttct gtcatggagg
      121 tccatataac atgttttgcc gccgcgtagc gtatccaaaa tcttgagctt cgtggcgtga
      181 ccgcctctgc acggtcatgc tacaaggact ggcttatgtc ccattcgttt caatccatcg
      241 cgctgtttgg caacgccaag gaccagcgcg tggccgaatg catgctgagc ctcgtcagtc
      301 acttccattc gcagggcttg cgccccctgg tcgatcccga ggtcggcctg gcatttcagc
      361 ccgacagcgt gctgccttgt ccggaagcgg ctttcgccac ccgggcggac ctcatcatcg
      421 ccgtcggcgg cgacggcacc ctgctctacg ccgccaggct cgtcgccggg cattcggtgc
      481 cgctgctcgg aatcaatcgc ggccgtttgg gtttcctcac cgatgtcagt cccaactcca
      541 tgctcgagga cgtggacgcc gtgctcgcag ggcgctacag cgaagatcga cgctcgctgc
      601 tggccgcacg cttggagaag agcggtgtcg agtcggtgcg cgcgctggcg ctcaacgacg
      661 tggtggtcaa caagtgggaa accggccgca ccatggactt cgaaacatcg atcaacggac
      721 ggttcgtcaa ttcgcacggc ggcgacggca tggtgatcgc caccgcgacc ggctcgaccg
      781 cctatgcgct gtcctgcggc ggacccatcg tcgagccgaa tctcgatgtg tgggtgttgg
      841 cccccatctc tccgcacacc ttgagcgacc gccccatcgt cgtgcgcgcc gacagcaaga
      901 tccagctgcg catgtcggat cgcttcgagt cgcacgccca ggtcacgtgt gacggacgtg
      961 cgatcggcga tctcgaacag ggggataatc tgtatgtcga ggccgccgag gcgcaaatca
     1021 ccctgctgca tccgccgggc tatgactact atcggctgct gcgctccaag ttgcactggg
     1081 gccgcggcgg ctacagcgac taaagcgcat gctctcccac ctgcaactac gcgattttgt
     1141 tctcgtcgat cgagcggaac tcgagctcaa gcccggcctg accgcgctca ccggggaaac
     1201 cggcgccggc aaatccattg tcgtcgatgc gctgttgttg atcgccggcg gccgcgccgc
     1261 gggcgacgtc gtgcgccagg gcgcggaacg cgccgaggtg gcggcgagtt tcgacgcgct
     1321 gccggatgcg gcgcaggcgt ggctcgaggc gcagagcatc gatctcgacg gcgacctcgt
     1381 gctcaggcgg gtgatcgggg cggatggccg ttcgcgcgcc tatgtgaacg gccaggtggt
     1441 gccgattcaa gcactgcgcg aactggcgca actgctcatc gagatccacg gccaacaaga
     1501 attccagcgc ctggtgaatc gcggcgcgca gcgcgacatg ctggacgacc atttggcgga
     1561 tcgcgccctg ctgcccgcgg tgtccgagct
//
LOCUS       DASWZT010000035         3602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_43891, whole genome shotgun sequence.
ACCESSION   DASWZT010000035 DASWZT010000000
VERSION     DASWZT010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43891"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VGE92_00915"
     CDS             <1..580
                     /locus_tag="VGE92_00915"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HEY1312400.1"
                     /translation="IDAYHDPLIDFARTLDAPARAVRADSEDTVKAVITKNSALIAKA
                     RFALDGKGTYPDATFTLRLSYGAVAGYQENGHSLAPTTNFAGAYEHATGRDPFKLPPT
                     WLAAEKAVDPAAQLNFTSTNDIIGGNSGSPVIGRGGEVIGLVFDGNLQSLGGDFGYDG
                     SQNRGISVDVTGITEALKKIYHADRLAKELTR"
     gene            591..2867
                     /locus_tag="VGE92_00920"
     CDS             591..2867
                     /locus_tag="VGE92_00920"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1312401.1"
                     /translation="MQPIEVTERLLQDATEQHRAGNFAAARALYREVLVRAPEHGVAL
                     YRSGLLELQCGAPAAALALVQRATAPAGDEPRYRFVQAQALQALQRWDEAIVAYLAAL
                     AAQPDFLDAWNNLGICRHLSRRLPAAAEAYRQALTLAPGNAGVMANLGAVLREMGSLD
                     EAVELLRAAADLEPQATSHAVNLGIAYWDQGDFSAAERILNGALQRAPDDADAAFNLG
                     NALHALGRTREAVERYRHAAALRPDFAEALVNLGNVQTEIGEFDAAMAAYEAALRAQP
                     RSIAALNNAACLMRLLGRIAEAQQLLARALELDPKNAALHDTLGNLYKDAGDLESAIE
                     SFRESLAIDPNSAPTHSNLAYALSFQQFEPGPILDECLRWNQRFATPLSVAAAPRPTD
                     LDPHRRLKIGYVSPDFRVHCQSLFTLPLLSRHDHARFEVFCYSSVKRADEYTRRLAGL
                     ADHWCEVRPLTDAALCRRIQDDGIDILVDLTMHMAGGRPLLFAAKPAPIQIAWLAYPG
                     TTGIAAMDYRISDPRLDPEGFDTHYSERTLRLPDSFWCYDPLDDQTRVNALPAIERGY
                     LTFGCLNNPCKITERTLCLWSEVLRALPTARLRLMAPPGQYREQLALTLAAYGVAAAR
                     IEFRAFQPRHVYLRSYHEIDVCLDTFPYNGHTTSLDSLWMGVPVVTRVGHTSVGRGGL
                     SQLFQLDLLELAAQSDAEFVDIAASLGKDLIRLAGLRATLRSRLNDSPLMDAARFARH
                     LERAYREAWHAYCAARAR"
     gene            3090..>3602
                     /locus_tag="VGE92_00925"
     CDS             3090..>3602
                     /locus_tag="VGE92_00925"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HEY1312402.1"
                     /translation="MVPTTSRVLSLSVSAALLAACGGGGSGAGTTSTNSTPPTQQTGT
                     VAIMISDASSEDWAAIDVNILSIALVPQGGGDNVTVYTAPAGGSMLNLAQLDQLAEIL
                     GNASVPVGTYTAAVLTISANPGDVALVVAADPEEGFPINPGTSIPADQIQIQHKQGSA
                     PNLTVPVTVDF"
BASE COUNT          636 a         1181 c         1119 g          666 t
ORIGIN      
        1 gatcgatgca taccacgatc cgctgatcga ttttgcgcgc acgctggatg cgcctgctcg
       61 cgccgtccgc gccgattcgg aagacacggt caaggcggtc atcaccaaga actcagcact
      121 cattgccaag gcgcggttcg cgctcgatgg gaaaggtacc tatcccgatg caacctttac
      181 gctgcgcttg tcgtacggag ccgtcgccgg ctaccaagag aacggccaca gcctcgcgcc
      241 gacgacgaat tttgccgggg cgtacgagca cgcgacggga cgtgacccgt tcaagttgcc
      301 gcccacctgg ctggcggcag aaaaagcggt cgatccggcg gcgcagctca acttcacctc
      361 caccaacgac atcatcggtg gcaattccgg ctcgccggtg atcggacgcg gcggcgaggt
      421 gattggcctg gtcttcgacg gcaacctgca atcgctgggt ggcgacttcg gctatgacgg
      481 cagccagaat cgcgggatat ccgtcgacgt caccggcatc acggaagccc tgaagaagat
      541 ttatcacgcc gatcgactcg cgaaagaact cacccgctag cggcgcgccg gtgcagccga
      601 tcgaggtcac cgagcgattg ctgcaggacg cgaccgagca gcatcgcgcc ggaaacttcg
      661 ctgcggcgcg agcgctgtac cgggaggtgt tggttcgtgc cccggagcac ggcgtggcgc
      721 tgtaccgcag cggcctgctc gagctgcagt gcggcgcgcc ggccgccgcg ctggcgctgg
      781 tgcagcgagc cacggcgccc gccggcgatg agccccgtta tcgattcgtg caggctcaag
      841 cgctgcaggc gctgcagcgt tgggacgagg cgattgtcgc gtatctagcg gcattggcgg
      901 cgcagcccga ttttctagat gcgtggaata atcttggcat ctgccggcat ttgtcgcgcc
      961 gccttcccgc ggcggcagag gcctaccgcc aggccttgac actcgcgccg ggcaatgccg
     1021 gcgtcatggc caacttgggc gcggtgctgc gcgagatggg ttcgttggac gaggccgtcg
     1081 agctgctgcg ggcggcggcg gatctcgagc cgcaggcaac ctcccatgcc gtcaatctcg
     1141 gcattgccta ttgggatcag ggcgatttct cggcggcaga acggattctc aacggtgcgc
     1201 tgcagcgggc gccggacgat gccgatgcgg cattcaactt gggcaacgcc ctgcacgcac
     1261 tcggccgcac gcgcgaagcc gtcgagcgct atcgacacgc ggcggctttg cgccccgatt
     1321 ttgccgaggc gctcgtcaat ctcggcaacg tacaaacgga gatcggcgag ttcgatgccg
     1381 ccatggctgc ctacgaggct gcattgcgcg cccagccgcg ctcgattgcc gcactgaata
     1441 acgctgcatg cctgatgcgc ttgctcgggc gcatcgcgga ggcgcagcag ttgttggccc
     1501 gggcgctcga gctggatccc aagaatgcag cgctgcacga tacgctcggc aacctatata
     1561 aagatgccgg cgacctcgag tcggcgatcg agagctttcg cgaatctctc gcgatcgatc
     1621 ccaactcggc gccgacgcat agcaatctgg cttacgcgct ctcgtttcag cagttcgagc
     1681 ccgggccgat tctcgacgaa tgcctgcgtt ggaaccaacg cttcgccacg ccgctgtcgg
     1741 tcgccgcagc gccgcgacca accgatctcg acccgcaccg ccgcttgaag atcggctatg
     1801 tctcgccgga ctttcgcgtt cattgccaaa gcctgttcac gctgccactg ctgtcgcggc
     1861 acgaccacgc gcggttcgaa gtcttctgct actcgagcgt caagcgcgcg gacgaatata
     1921 cgcgccgcct cgccggcttg gccgatcatt ggtgcgaagt tcggccgctg actgacgcgg
     1981 cgctgtgccg acgcatccaa gatgacggca tcgacattct ggtcgacttg accatgcaca
     2041 tggcgggcgg acggccgttg ctgttcgcgg ccaagccggc gccgattcaa atcgcatggt
     2101 tggcctatcc cggtaccacc ggaatcgcgg cgatggatta ccgcatcagc gatccgcggt
     2161 tggatcctga aggtttcgac acccattaca gcgagcgcac gctgcgttta cccgattctt
     2221 tctggtgtta cgacccgctt gacgatcaga cgcgggtcaa cgcattgccc gcgatcgaac
     2281 gcggttacct gaccttcggc tgcctgaata atccgtgcaa gataaccgag cgcacgctgt
     2341 gcctgtggag tgaggtgctg cgtgcgctgc cgacggcgcg cttgcggctc atggcgccgc
     2401 cgggccaata tcgcgagcag cttgcgctta cgctagccgc gtatggcgtc gcggccgcgc
     2461 gcatcgaatt tcgcgccttt caaccgcgcc acgtttactt gcgctcgtat cacgaaatcg
     2521 atgtgtgtct cgacaccttt ccctacaacg gccacaccac cagtctcgat tcgttgtgga
     2581 tgggtgtccc cgtggtcacg cgcgtgggcc acaccagcgt cggtcgaggc ggtctcagcc
     2641 agttgtttca actcgacctg ctcgaactcg cagcccaatc cgacgccgag ttcgtcgaca
     2701 ttgcggcatc actcggcaag gatctcattc gactcgccgg cttgcgcgcg acgctgcgct
     2761 cgcggctcaa cgattcgccg ctgatggacg cggcgcgatt tgcccgacac ctcgagcgcg
     2821 cctatcgcga ggcatggcac gcgtactgcg cggcacgcgc tcgatagttt cagcgttcgg
     2881 atcggagtcg atgatttccc gcgttatgta atcactcgcc gcgagtcgcg agatcgccgt
     2941 ctcacctcga gcactgcgtc tgcaattggc gacgcaaatc actgtcaacg caggctgtga
     3001 tgagccgttg gtgcagacaa cagttgcgca acggtgcgcg cttgagtttt cagggaaaaa
     3061 ttatcgtcaa cttcgaggag acaggaatca tggtcccgac tacatctcga gtattgtcgt
     3121 tgagcgtgag cgccgcgttg ctcgccgcat gcggcggcgg cggttccggc gccggcacga
     3181 cgtccaccaa ctccacgccg ccgacgcagc agaccggcac cgtggccata atgatcagcg
     3241 atgcgtcgag cgaagattgg gcggcaatcg acgtgaacat cctgtcgatc gcactcgttc
     3301 cgcagggcgg cggcgacaat gtcaccgtgt acacggcccc cgccgggggc tcgatgctca
     3361 atctcgcgca gctcgatcaa ctcgcggaga ttctcggcaa cgccagcgtg cccgtgggca
     3421 cctacacggc cgccgtcctc accatcagcg ccaatccggg cgacgtcgca ctcgtcgtcg
     3481 ccgcggaccc tgaagagggc tttccgatca atcctggaac gtcgattccg gccgatcaaa
     3541 ttcaaatcca gcacaagcag ggtagcgcgc cgaatctcac ggtgccggtc acggtcgact
     3601 tc
//
LOCUS       DASWZT010000036         2172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_43900, whole genome shotgun sequence.
ACCESSION   DASWZT010000036 DASWZT010000000
VERSION     DASWZT010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43900"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..1386
                     /gene="egtB"
                     /locus_tag="VGE92_00930"
     CDS             142..1386
                     /gene="egtB"
                     /locus_tag="VGE92_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEY1312403.1"
                     /translation="MSHAPRGGSLGPRSVAESPDEARAYWRDAFRRVRAETEARASHL
                     SAEDQIVQSMADASPAKWHRAHVTWFFEQFLLVPNDPRYQIFDQRFPFLFNSYYVAAG
                     PRHARPQRGLITRPNGDDVTGYRAHVDSAVERLIDNVPAANAAHVFNILEIGLHHEQQ
                     HQELLITDILHAFAQNPTDPVYDTAWQPPRASQGTGGFVDVPSGIMTIGHEGNGFCFD
                     NETPRHDELIPDVRIARHLVSNAEWLEFIAAGGYSTPSLWLSDGWATVQAEGWQAPGY
                     WRQHDGGWHAMTLGGLKPVDPAAPVTHISYYEAEAFARFAGKHLPSEAEWEVAAGAEL
                     IADAFGIAWQWTRSAYLPYPAYRAAEGALGEYNGKFMVSQMVLRGSSLATPEGHERVS
                     YRNFFYPSARWQFSGLRLAEFS"
     gene            1396..>2172
                     /gene="egtD"
                     /locus_tag="VGE92_00935"
     CDS             1396..>2172
                     /gene="egtD"
                     /locus_tag="VGE92_00935"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR03438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEY1312404.1"
                     /translation="MVALARSHRVQPQNVEASAFEVDVLAGLSATPKRVAAKYFYDGT
                     GSLLFERITELPEYYPTRCEMSILGDNAAEIAKLIPQGAALVEFGSGSSKKVRILLEA
                     APSLAAYVPVDICGEMLEQEAIDLQPDFPRLKVLPVRADITKAFELPPKAIAAPVRIG
                     FFPGSTIGNFEPHEACAFLRNAGRILGPGATLIVGADLIKPVEALNAAYNDAAGVTAK
                     FNLNLLVRMNRELRATFKLDTFEHHAFFNRERHRVEMHLAS"
BASE COUNT          414 a          703 c          654 g          401 t
ORIGIN      
        1 ggttacgggc gcgggcccga ttcgaggttg ccccgacccc ctgccgatgg aacaattgca
       61 tccattggac gtctatgtcc gtaaatctac tgccaactac cttcgaataa agcgacggag
      121 accccaatgg cgcgcaccgc aatgtcgcac gctccgcgcg gcggcagcct cggaccacgc
      181 tcggtagcgg aatcgccgga cgaggcgcgg gcttactggc gcgacgcttt ccgccgcgtg
      241 cgcgccgaga cggaagcacg cgcgtcgcat ttgtcggccg aggaccagat cgtacagtcg
      301 atggccgatg ccagcccggc gaaatggcac cgcgcgcacg tcacttggtt ctttgagcag
      361 tttctgctgg tgccgaacga tcccaggtac caaatcttcg accagcgttt tccattttta
      421 ttcaattcct attacgtcgc cgccgggccg cgccacgcgc gtccgcaacg cggcttgatc
      481 acgcggccga acggcgacga cgtgaccggc tatcgcgctc atgttgactc ggcggtcgaa
      541 cggctgatcg acaacgtgcc ggcggcgaat gccgcgcacg tgttcaacat tctggaaatc
      601 gggttgcatc acgagcagca acaccaggaa ctgctgatca ccgacatcct gcacgccttt
      661 gcgcagaacc cgaccgaccc ggtttacgac accgcgtggc aaccgccgcg cgcgagtcaa
      721 gggacgggcg gctttgtcga tgtgccgtcc ggaatcatga ccatcggtca cgaaggcaac
      781 ggtttctgtt tcgacaacga gacgccgcgg cacgacgagc tcattcccga tgtgcggatc
      841 gcccgccatc tagtcagcaa cgccgaatgg ctcgaattca tcgcggccgg cggctacagc
      901 accccctcac tctggctatc ggatggctgg gctacggtgc aggccgaggg ctggcaggcg
      961 cccggctatt ggcgccaaca cgacggcggc tggcacgcga tgacgctcgg cggcctcaag
     1021 cccgtcgatc ccgcggctcc cgtcacgcat atcagctatt acgaagccga ggcctttgcc
     1081 cgttttgccg gtaaacactt gcccagcgag gcggaatggg aagtcgcggc cggcgcggaa
     1141 ttgatcgccg acgccttcgg catcgcttgg caatggacgc gcagcgccta tctaccctac
     1201 ccagcctacc gcgcggccga aggggcgctc ggcgaataca atggcaagtt catggtcagc
     1261 cagatggtgt tgcgtggatc ttcactagca acaccagaag gccatgagcg cgtcagctat
     1321 cgcaattttt tctatccgtc ggcgcgctgg caattcagcg ggctgcggct cgccgagttt
     1381 tcttaaggag cagtaatggt cgcgctagca cgttcccacc gcgttcagcc gcaaaatgtc
     1441 gaagcctccg ccttcgaggt cgacgttctc gccggcttga gcgcgacgcc gaagcgggtg
     1501 gccgcgaaat atttttacga cggcaccggc tcgctgctgt tcgagcgcat caccgaactg
     1561 cccgaatact atccgacgcg ttgtgagatg agcatcctgg gcgacaacgc cgccgaaatc
     1621 gcaaaactca ttccgcaagg cgcggcgcta gtggaattcg gctcgggctc gagcaagaag
     1681 gtgcgcatcc tgttggaagc ggcaccgagt ctcgcggcct acgtgcccgt cgatatttgc
     1741 ggcgagatgc tggagcagga agcaatcgac ctgcagccgg attttccgcg gctcaaagtg
     1801 ctgccggttc gggccgacat cacgaaggcg ttcgagttgc cgccgaaagc gattgccgcg
     1861 ccggtacgga tcggattttt tccgggttcc accataggca atttcgagcc gcatgaagcg
     1921 tgcgcgttcc tgcgcaatgc gggacgcatc ctgggcccgg gcgcgacgct gatcgtcggc
     1981 gccgacctga tcaagccggt cgaggctctc aacgccgcct ataacgacgc cgccggtgtc
     2041 accgccaagt tcaatctcaa tcttctggtg cgcatgaatc gcgaactgcg cgccaccttc
     2101 aagctcgata ctttcgagca tcacgcgttc ttcaaccgcg agcgccatcg agtcgaaatg
     2161 catctcgcca gc
//
LOCUS       DASWZT010000037         3484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_24533, whole genome shotgun sequence.
ACCESSION   DASWZT010000037 DASWZT010000000
VERSION     DASWZT010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24533"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..443)
                     /locus_tag="VGE92_00940"
     CDS             complement(105..443)
                     /locus_tag="VGE92_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312405.1"
                     /translation="MYLRKIVLIGACAAVLAPAISNASPERTALNACVRAFATSLATP
                     GNAAPAVKVDYRGDITSGSVLDYFASEYTFDLHANNGKTGLAVGHVSCTSDTRGAVIS
                     MSQVARAAKN"
     gene            complement(571..1464)
                     /locus_tag="VGE92_00945"
     CDS             complement(571..1464)
                     /locus_tag="VGE92_00945"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY1312406.1"
                     /translation="MVDPMRLPSLKFLKTFQVAARLQSFKAAAEELFVTPSAVSHQIK
                     ALEEQLGVALFERGVRTLKLTDAGAHYLEHINDIFSKLESVTEQLQERYGRSIIRLNV
                     PSFFANELLLPRLSSFSQAREETDIRIETTVSAPKTHPPEADLSIVVGSGPWDGLTVH
                     ELFAQSFIAACSPAFLLENPINAYADLNGKTLLLHEERREAWERWAVGLGIEPIKPNR
                     LVRLDTMSAVVLAAEQGVGVALVPSRLSADRFVAGGLVKLFDAELTTNESYVLLHRPE
                     DRGREDLQELTQWILSECRVA"
     gene            complement(1693..2760)
                     /locus_tag="VGE92_00950"
     CDS             complement(1693..2760)
                     /locus_tag="VGE92_00950"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEY1312407.1"
                     /translation="MATDTKSAVVAHRKGFYYEVKAGKRYLWCRCGRSQTQPFCDGSH
                     AGSGFLPIAFLAARDEDVIFCGCKQTSTGPFCDGTHSNLPGGYLPDDPESAANRNVAT
                     VDPGASPVVPLDGACYVFSTARAPLMDRGGMRYCPVVSPALGALFQSQFYAEFAEGVS
                     PVISADGRHTVLFVTQGDGDVEISGRRFALTALTGIYVRPEEAYRVHNRSPAALKCFI
                     SNGPGSEELVWGDAMPMNFETQYPQRAARIDPAQRHKMAERYYQVLVNRDQGSTLMTQ
                     FIGNIPLSKAEPHRHLYEEALIFLNGAGVVWTENLKTPVAAGDVLFLPRKQVHSVQCL
                     TPGGFDIVGVIYPGDNPSINY"
     gene            complement(2785..3267)
                     /locus_tag="VGE92_00955"
     CDS             complement(2785..3267)
                     /locus_tag="VGE92_00955"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY1312408.1"
                     /translation="MARAQLKKNSAKAPRQALDQPLFDDFLPHLIARLAYQLNGDLSD
                     KLRREGINVTRWRILAVLAMGDGITIKEMVDRAMMQQPALSRALMRMEEEKYVRRVLA
                     SGDARYVEVYLTDKGRALFDALNAVVRRRQQRLVKGFSARELAAAFTLIRRLSRNMLF
                     "
     gene            complement(3269..>3484)
                     /locus_tag="VGE92_00960"
     CDS             complement(3269..>3484)
                     /locus_tag="VGE92_00960"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312409.1"
                     /translation="EYLRALEQGAITPQHILGEIGEVANGTKFGRRAPGDVTLYKSLG
                     IAPQDLAAAHTVLEKARAAGLGQVIDF"
BASE COUNT          686 a         1084 c         1072 g          642 t
ORIGIN      
        1 gggcggccgc agcggcagcc gccgcatcgt tcgatgtgat ctgcatcgcg gatttatacg
       61 ctggcggcga tcaacccgcc agcgagcatg agcgatcgct gcgcttagtt cttcgcggcg
      121 cgcgcaactt gcgacatgga gatgaccgca ccgcgcgtat ccgacgtgca gctcacgtgt
      181 cccaccgcga gaccggtctt gccgttgttg gcgtgcagat cgaaggtgta ttcgctcgcg
      241 aaataatcga gaaccgaacc ggaggtgatg tcgccccgat agtcgacttt gactgcggga
      301 gctgcatttc caggcgtggc aagactcgtg gcgaaggccc gtacgcaggc attcaacgcg
      361 gtccgctcgg gcgacgcgtt cgaaatggcg ggagcgagca cggcagcgca ggcgccgatc
      421 agaacgattt ttcgaaggta catggtggta ttccttgatg tcgaaaatac caacgcccca
      481 gatgtacgat aggcaacgcc tacgcgcgcg acaaacgaga aatttcaacc aaatcagcaa
      541 tctttttcac ttgtaggatt ttgtccgaca tcacgccacg cggcattcgc tcaggatcca
      601 ctgcgtcaac tcctgtagat cttcgcgccc gcgatcttcc ggccggtgca ataagacgta
      661 gctctcattc gtggtcaatt ccgcgtcgaa caacttcacc aagccgccgg cgacgaagcg
      721 gtcggcgctc aggcgcgagg gcacgagtgc cacgccgacg ccctgctcgg cggcgagcac
      781 caccgccgac atggtgtcga gccgcaccag ccgattgggc ttgatcggtt cgatccccaa
      841 cccgacggcc cagcgctccc acgcttcgcg ccgctcttca tgcagcagca acgtcttgcc
      901 gttcaagtcg gcgtacgcat tgatgggatt ttccagcagg aaggcgggtg aacacgcggc
      961 gatgaagctt tgcgcgaaca attcgtgaac cgtcagccca tcccagggcc cggacccgac
     1021 gacgatggac aggtccgcct cgggcggatg cgtcttcggc gcggagacgg tcgtttcgat
     1081 gcgaatgtcc gtctcttcgc gggcctgcga aaatgaggac aaacgcggca gcagcagttc
     1141 attggcgaaa aacgacggca cattcaagcg gatgatgctg cgcccgtagc gctcttgcag
     1201 ctgctcggtc accgattcga gtttcgagaa aatatcgttg atgtgttcga ggtagtgggc
     1261 gcccgcatcg gtcaacttga gcgtgcgaac gccgcgctcg aacaacgcga ccccgagctg
     1321 ttcctcgagc gccttgatct gatggctcac ggccgatgga gtgacgaaca actcctctgc
     1381 ggcggccttg aagctttgca gcctggccgc gacctggaag gtcttcagaa acttaagcga
     1441 cggcaagcgc atagggtcaa ccaatacttg gaaatggggc gtgaactacc gttgcggccc
     1501 cacggcatac ttcgatatat catacaccat gactcgagca acattcgtgc cagcaggacc
     1561 ggtgggtaaa tgccgggatt tgccgacttt gaggtgactt aatcccgctt aacgccatgt
     1621 tcgcgcgcat cgggcgagcc gcaccgcacg atagtagcgc agggattgtt acgaaatggc
     1681 ggcggcgctg ccctagtagt tgatcgacgg attgtcgcct ggatagatga ccccgacgat
     1741 gtcgaacccg ccgggcgtca agcactgtac cgagtgcacc tgcttgcgcg gcaggaacag
     1801 cacatccccc gcggcgaccg gggtcttgag gttctcggtc cacacgacgc cggcgccatt
     1861 gagaaaaatg agcgcttctt cgtacaaatg ccggtgcggc tcggccttcg ataaggggat
     1921 attgccgatg aactgcgtca tgagcgtcga accctgatcg cggttgacca acacctggta
     1981 gtagcgctcg gccatcttgt ggcgctgtgc aggatcgatg cgcgcggcgc gctgcggata
     2041 ttgcgtctcg aaattcatcg gcatcgcatc gccccacacc aactcctcgc tgccgggtcc
     2101 gttcgaaatg aagcacttca acgccgccgg gctgcggttg tgcacgcggt aggcttcttc
     2161 cggccgcaca tagatgccgg tgagcgccgt cagcgcgaat cgacgcccgc tgatctcgac
     2221 gtcgccatcg ccttgtgtca cgaacagcac cgtgtgccgc ccgtccgccg aaatcaccgg
     2281 cgaaacgccc tcggcaaact ctgcatagaa ctgcgattgg aacaaggcac cgagtgcggg
     2341 actcacgacc gggcagtagc gcatgccgcc gcggtccatc agcggcgcgc gcgcggtgga
     2401 aaatacatag cacgcaccgt cgagaggcac caccggcgat gcaccgggat cgaccgtggc
     2461 gacgttgcga ttcgcggcgc tctcgggatc gtccgggaga tagccgccgg gcagattgct
     2521 atgggtgccg tcgcagaaag gtccggttga ggtctgcttg caaccgcaga agatgacatc
     2581 ctcatcgcgc gcagcgagga aggcgatcgg cagaaaaccg ctgcccgcat gcgagccgtc
     2641 gcagaacggc tgtgtctggg agcggccgca gcggcaccac aggtagcgct tgcccgcctt
     2701 gacttcgtaa tagaagccct tgcgatgcgc gacgactgca gatttggtat cggtagccaa
     2761 gagttcgtgt ccgctcgcag gaagtcaaaa cagcatattg cggctcaggc ggcgaatcag
     2821 cgtgaacgcc gccgccagtt cccgtgcaga aaatcccttc accaagcgct gttggcgccg
     2881 gcgcaccacg gcgttcaacg cgtcgaacaa cgcccggcct ttgtcggtca gatacacctc
     2941 gacatagcgg gcgtcgccgc tcgccaggac gcgccgcacg tatttctcct cctccattct
     3001 catcagcgcg cggctcagcg ccggctgctg catcatggca cggtcgacca tttccttgat
     3061 ggtgatgcca tcgcccatgg cgagcaccgc gaggatgcgc cagcgcgtga cattgatgcc
     3121 ttcgcggcgc agcttgtcgc tgagatcgcc gttcaactga tacgccagcc gcgcgatcaa
     3181 atgcggcaga aaatcgtcga acagcggctg atccagcgct tggcgcggcg cttttgcgga
     3241 atttttcttc agttgggcgc gcgccatgtc agaagtcgat gacctgaccg agaccggcgg
     3301 cgcgcgcttt ctcgagcacg gtgtgcgccg cggcgagatc ctgcggcgcg atgccgagag
     3361 acttgtacaa ggtgacatcg ccgggcgcgc gccggccgaa cttggtgccg ttggccacct
     3421 caccgatctc accgagaatg tgctgcggcg tgatggcacc ctgctcgagc gcccgcaggt
     3481 actc
//
LOCUS       DASWZT010000038         9728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_369, whole genome shotgun sequence.
ACCESSION   DASWZT010000038 DASWZT010000000
VERSION     DASWZT010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9728
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VGE92_00965"
     CDS             complement(<1..947)
                     /locus_tag="VGE92_00965"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY1312410.1"
                     /translation="MAVNKLQAMQVFVRIVETGGVTRAADSLQMPKATATSLIQQLEA
                     SLGVKLLNRTTRRVSVTTDGAAYYPRCLAILTEVRETEESLAQHHAAPQGRLRVEVPT
                     LIARLVIVPALPVFFARYPEIELQLGCSERRADLIEEGIDCALWSGDLEDSTLIARRV
                     GQLYFGTCASPSYLAAHGEPHHPDELKQHRCINHFSPRTGRIFDWVFAKNGARLQTTL
                     RGHIALDDENSYLAAAEAGLGIAQIPAFVLKDAMERGQLELLLGDWFPEPAPINVVYP
                     QNRHLSNKIRVFVEWVAETFGEHDGIQLRSSLPARLPPRA"
     gene            1043..1366
                     /gene="trxA"
                     /locus_tag="VGE92_00970"
     CDS             1043..1366
                     /gene="trxA"
                     /locus_tag="VGE92_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEY1312411.1"
                     /translation="MNKIKHVTDTTFGSEVLESQLPVLVDYWAEWCGPCKMIGPVLDE
                     SAKNYAGRLDIAKINIDQNQAVPSRYRVRGIPTLMLFKNGEVVATKVGAVSKSQLAAF
                     IDAHL"
     gene            complement(1394..2377)
                     /locus_tag="VGE92_00975"
     CDS             complement(1394..2377)
                     /locus_tag="VGE92_00975"
                     /inference="COORDINATES: protein motif:HMM:NF008024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEY1312412.1"
                     /translation="MADQDMGMVVRTAGGAEALEWVALNRGIPGDGEVRIEQRAIGVN
                     FIDTYLRSGLYPWPSTPLIPGAEASGVVAELGPGVTDLKVGDRVAYVVSSGAYRTQRI
                     IAAERLVKLPDSISFDVAASIMLKGLTAQYLVNSAYTVKSGDTVLVHAAAGGVGLLLG
                     QWLRALGAIAIGTAGSSEKAAIAKANGYTHVIDYRNEDFAARVREITQGQGCQVVYDS
                     VGKDTWRGSLKSLRPRGMFVHFGQSSGMIADFKFSDLAAGGSLFATRPSVFDYIKRRE
                     ELVARAADLFARLTKGEIKAHIGQRLPLRDAAQAHRELEGRRTIGATVLAP"
     gene            complement(2380..3549)
                     /locus_tag="VGE92_00980"
     CDS             complement(2380..3549)
                     /locus_tag="VGE92_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY1312413.1"
                     /translation="MHYSPLVKRIAGDGADAWRIHYEARAAAERGEDVVLLSVGDPDL
                     ESPAPVLERAIERLRAGDTHYTPATGREHLRAAIAAAHRARTGQPVDASNVIFLAGAQ
                     NALFVTSLMVAGSGDELIAVEPLYPSYPATLQISGARMVRVPAPAANGFRVDLAALEA
                     AITPRTRAVFFATPNNPSGVILSKEDLAVIGSLARRHSLWVVADEVYAGLAPGGRVPS
                     LAAELPEQVVTISSLSKSHAMPGWRAGWLVGPPQLVMHAEAMAQCMLFGLPGFIQEAA
                     VTALGVAAAAEARVRDYCAVRRDLLLAGLEGIRGLKSHVPDAGMFLLVDVRATGLSGY
                     DFMCELYRTERVSVLDGGAFGQGTSGFVRVCFAADASLLEKAIIRIRRFVESLGS"
     gene            3629..5248
                     /locus_tag="VGE92_00985"
     CDS             3629..5248
                     /locus_tag="VGE92_00985"
                     /EC_number="4.1.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF005712.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-guanidino-2-oxopentanoate decarboxylase"
                     /protein_id="HEY1312414.1"
                     /translation="MSRNVGAYLVELLAANDIDTVFGIPGVHNLELYRGLAASNIRHV
                     LVRHEQGAGFAADGFARLSGRPAAAFVISGPGLTNILTAVAQAYSDSVPLLVIASAPV
                     RASFRKQWGVLHELYDQAALAAGVFGVARTAFSAEDVRDHLRLCLASFCTGRPRPAYL
                     EIPLDVLAEGTTLRAQRFEGGTLMPQASPEQIDAAVRVLELAARPLVIAGGGARHAGA
                     ELHRLVETLDCPLVTTAAGKGVLAESHPANFGTSLPYRCIQQLIVDADLVLAVGTELG
                     ETDIYSATKLPLGGMLVRIDVDPVKLSDQYSADVCVWSDARSALRAINHALANRLPES
                     AVRSRWRTALGDGAKIRSEIDNGFDAKARAQWHALRAIKAALPWDGVVFSDMTQIAYL
                     GNYAYPVDNPGQWFHPSGYGTLGFALPAALGAKIACPARAVIALAGDFGLQFTLPEIM
                     TAIETGISLPIVVWNNSALGQIRDDMVAAGIAQLGVIARNPDFVSLARACGAEGYRVH
                     DANALTEAIRTALSHTGPTLIEVVATSFSIT"
     gene            5260..5691
                     /locus_tag="VGE92_00990"
     CDS             5260..5691
                     /locus_tag="VGE92_00990"
                     /inference="COORDINATES: protein motif:HMM:NF018098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allene oxide cyclase family protein"
                     /protein_id="HEY1312415.1"
                     /translation="MKITSCLTALAATLLTHSAAAAEQIKVVEHPVGETTVDLGAKGD
                     SIGDLLVFANGVFDSANKVQIGSDQGYCVRTVVGKSWECMWTLLLKGGQITVEGPFMD
                     EGDSLFAVTGGTGKYAGAKGSMKLHARDAKASSYDFVYDLL"
     gene            5691..6911
                     /locus_tag="VGE92_00995"
     CDS             5691..6911
                     /locus_tag="VGE92_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HEY1312416.1"
                     /translation="MAYTHEDCLRARQDFPALRRAPHLAYLDGPAGSQVPRAVVEAIA
                     DVYASCNVNTRGNFAPSREVDRRLDAARGAVAAFLGAESGACISFGQNMTTLNFALSA
                     AIGRTLHKGDEVLITQLDHEANRGPWIGLEERGVVVREVRVLESGELDYADMAARISP
                     RTKLFALGASANSLGTVNDLALARRLTHEVGALLVIDAVHYAPHFPIDVQALGADFLL
                     CSAYKFYGPHVGVLYSRPGALDRLPTNRLSVQDPAAPYRIETGTLNHAAIDGVRAAVE
                     YIAGWGSGASLRERIVSAMTQIAAYEHGLAKSYFDAAARMPGVRVWGPDFTAGRARAP
                     TVSITLAKITADAAAGVLGEQGICVWDGNFYAARPAQMLGFTERGGLLRTGVSMYNTR
                     DEIDRLLSGLERLL"
     gene            complement(6906..8291)
                     /locus_tag="VGE92_01000"
     CDS             complement(6906..8291)
                     /locus_tag="VGE92_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEY1312417.1"
                     /translation="MAIVGAGFSGTVLAANLLRRPPSYATDVVLIEQAPDIGRGVAYA
                     ARDYPYLLNVPAARLSADAQDPLQFLRFARRHMPGADGEDFLPRALYGDYIRDVLLQA
                     ERAAPAHVRLVRLCDEVRHITRRGGDKPFAVEFADRPPLAADRVILAVGSPPPSLPPW
                     AAAVRDHHAYRHDPWALPKRLTHTHSVLIVGNGLSMADAAVALSEDPSRAPLLHTISR
                     RGLVPLPQATLRPSAAPGDGVAMLACAHSLRRVLAMAREWTRQAEQNGGDWREVVTFI
                     RQLAPALWSRLPQAERRRFVRHLQPHWDVHRHRMPPDMGARIDGLRSSGKLRVNAGRI
                     DSVTADGDRLHVFWRPRGGRSSATLIVDCVVNATGPGYGLERSTNPLLSSLHQAGLVS
                     EDALNLGLRTGRFGACIDMHGVPSEGLYYLGPMLRANHWEATAATELRDHARELASHL
                     VEMPGPRAQGS"
     gene            8461..9228
                     /locus_tag="VGE92_01005"
     CDS             8461..9228
                     /locus_tag="VGE92_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1312418.1"
                     /translation="MTFAVTLPAFADTDPTVDQIYAEARSGHLEQAQQMMNQVLADHP
                     NSGRAHYVQAELYAREGKTALARSELATAEQLKPGLPFESQRSVQELKAQVGLGSRPA
                     TPIGMRSESVAPRFPLGTVLILVAAVGLLWMLFRRRSYSQYPAAGPGVGGTPGYGPGP
                     GYGPAPMGGGGIGSGIAGGLASGLAVGAGVVAGEELAHHFLDGGRGGGVIPPAEAGDS
                     GVSNSDMGGSDFGMSDPGAGWDDGGGSDGGGGGGDWS"
     gene            complement(9289..>9728)
                     /locus_tag="VGE92_01010"
     CDS             complement(9289..>9728)
                     /locus_tag="VGE92_01010"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY1312419.1"
                     /translation="SCKVAATTPKLLLERYPNKVRFIFRHFPVQDAHPHALMAAEVAE
                     AAAAQGKFWQMHDLLFQNQMHLKEKDLYRHAAELGLDMARYTAEMDDHIYLQKVREHI
                     EGARRSHIRATPTFFIDGVVQDISFGMKALHDAVATAVARASH"
BASE COUNT         1775 a         3121 c         3054 g         1778 t
ORIGIN      
        1 gcgcgcggcg gcagccgcgc cggcagcgag gagcgcagtt ggatgccgtc atgctcgcca
       61 aaggtctcgg caacccactc gacgaacacg cggatcttgt tggacagatg ccgattttgt
      121 ggataaacca cattaatcgg cgcgggttcg ggaaaccagt cgccgagcaa tagttccagc
      181 tggccgcgtt ccattgcatc cttgagcacg aaggccggaa tctgcgcgat gcccaagccg
      241 gcttcggcgg ccgcgaggta gctgttctca tcgtcgagag cgatgtggcc gcgcaacgtc
      301 gtttgcaggc gcgcaccatt cttggcgaac acccagtcga agatccggcc ggtgcgcggc
      361 gagaagtgat tgatgcagcg gtgctgcttg agctcgtcgg gatgatgcgg ctcgccgtgg
      421 gctgccaagt aggacggcga ggcgcaggtg ccgaagtaga gctggcccac gcgacgcgcg
      481 atcaatgtgg aatcttcgag gtcgccgctc catagtgcgc agtcgatgcc ttcctcgatc
      541 agatccgcgc gccgctcgct gcagccgagt tgcaattcga tttcaggata gcgcgcgaag
      601 aataccggaa gcgccggcac gatgaccaat cgcgcgatca gggttggtac ttcgacccgc
      661 agccgcccct gcggagccgc atgatgctgc gcgagcgatt cttctgtttc gcgaacttcg
      721 gtcaggatgg cgaggcaccg tgggtaataa gcggcaccat ccgtggtcac gctgacccgg
      781 cgcgtggtgc ggttcaggag cttcacgccg agcgaggcct cgagctgttg aatcaatgac
      841 gtcgccgtgg ccttgggcat ctgcaggctg tccgccgccc gggtcacccc tccagtttcg
      901 acaatgcgaa caaatacctg cattgcctgg agcttgttaa cggccattgt ccatattatt
      961 gaataataag ttcatattgt catcctttaa taagtatttt tagacaatac actgtggttg
     1021 tcgtcaggag caggaaacat ccatgaacaa gatcaaacac gtcaccgaca caacttttgg
     1081 tagtgaagtt ctcgaatcgc aacttccggt gctggtcgac tattgggccg agtggtgcgg
     1141 cccgtgcaag atgattgggc cggtgctcga tgagtccgca aaaaactacg ccggtcggtt
     1201 ggacatcgcc aagatcaata tcgaccagaa ccaggcggtg ccctcgcgct atcgcgtgcg
     1261 cgggatccca acattgatgt tgttcaaaaa cggcgaagtg gtggccacca aagtcggcgc
     1321 cgtgtccaaa tcgcagttgg cggcattcat cgacgctcac ttgtaacgac gcgatgccgc
     1381 aggtacgcgt ctcttagggc gccagcaccg tcgcgccgat cgttcgccgg ccctcgagct
     1441 cgcgatgcgc ttgtgcagca tcgcgcagcg gcaggcgctg gccgatatgt gccttgattt
     1501 cacccttggt cagcctcgcg aacaaatcgg cagcgcgcgc gaccagctct tcgcgacgct
     1561 tgatgtagtc gaacacgctc ggtcgtgtcg cgaacagcga accgccggcg gcgagatcgg
     1621 agaacttgaa atccgcgatc attcccgagg actggccgaa atgcacgaac atgccgcgcg
     1681 gccgcaggct cttgagggaa cctctccagg tgtccttgcc gactgagtcg taaaccacct
     1741 ggcagccctg gccctgggtg atttcccgga cgcgtgctgc gaaatcctcg ttgcggtagt
     1801 cgatgacgtg cgtgtagccg ttggccttcg cgatagcggc cttctcggac gaaccggcgg
     1861 tcccgatagc gatcgcgcca agcgcccgaa gccattgtcc cagcagcaat ccaacgccgc
     1921 cggcggccgc atgcaccaac acggtgtcgc ccgattttac cgtgtaggcg gagttcacca
     1981 ggtattgcgc cgtcaaacct ttgagcatga tggatgccgc cacatcgaag ctgatcgaat
     2041 ccggcagctt gacgaggcgc tccgcggcga tgattcgctg ggttcgatag gcgcctgagg
     2101 acaccacgta cgcaacgcgg tcccccactt tcagatcggt caccccgggg cccaactcgg
     2161 cgaccactcc tgaagcctcg gcgcccggaa tcagcggcgt tgaaggccac ggatacaagc
     2221 cgcttctcaa atacgtgtcg atgaagttga cgccgatcgc tcgttgctcg atgcgcacct
     2281 cgccgtcgcc cggaatgccc cggttgagcg ccacccattc gagcgcctcg gctccgcccg
     2341 cggttcgcac caccatgccc atgtcctggt cagccatgcc taactcccca aagattccac
     2401 gaagcgccgt atgcggatga tcgctttctc gagcaaactc gcgtcggcgg caaagcatac
     2461 ccgcacgaag ccgctggtcc cctgaccgaa tgccccgcca tcgagcaccg atacacgctc
     2521 ggtccgatac agttcgcaca tgaagtcgta gcccgacaat ccggtggcac gcacatcgac
     2581 cagcaagaac atgccggcat cggggacgtg gctcttgagt ccgcggatgc cctcgaggcc
     2641 tgccagcaga agatcgcgtc tcacagcgca gtagtcgcgc acgcgcgctt cggcggccgc
     2701 ggcgacaccc aaggccgtga ccgcggcttc ttgaatgaat ccaggaagcc cgaacagcat
     2761 gcactgcgcc atggcctccg catgcatcac caactgcggc ggacccacca accatccagc
     2821 gcgccagccc ggcatggcgt gcgacttcga tagactgctg atggtcacga cctgctccgg
     2881 cagctcggcg gcgaggctgg gtactcggcc gcccggagcg agcccggcgt atacctcatc
     2941 cgccaccacc cacaaagagt ggcggcgcgc gaggctgccg atcaccgcca agtcctcctt
     3001 gctgagaatc actccgcttg gattgttcgg tgtcgcgaaa aacaccgccc gtgtgcgcgg
     3061 cgtgatcgcc gcttcgagcg ccgcgagatc gacgcgaaat ccgtttgccg ccggggccgg
     3121 cacacgcacc atccgcgcac cggaaatctg caacgtggcg gggtagctcg ggtatagcgg
     3181 ctcgaccgcg atcaattcat cgccggatcc cgcgaccatc agcgaggtga cgaataaagc
     3241 gttctgcgct cccgccagga agatgacgtt ggatgcatcg acgggctggc cggtgcgcgc
     3301 gcggtgcgcc gcggcgatcg ccgcgcgcaa atgctcgcgt cccgtcgccg gcgtgtaatg
     3361 cgtatcgccg gcgcggagtc tctcgatggc gcgttccaaa acgggcgccg gcgactcgag
     3421 atcgggatcg ccgacgctca gcaggacgac gtcttcgccg cgctccgccg cggcgcgcgc
     3481 ctcataatga atgcgccaag cgtcggcgcc atcgcctgcg atgcgcttga ccagcggtga
     3541 atagtgcatc ttttctcttg cctacccggt tgcagtgcgc aatcctaacg cgcttcgtaa
     3601 aattgcgttc aaattcgaga aaatgaacat gtcgcgcaat gtcggcgcat atctggtcga
     3661 actcctggcc gccaacgata tcgacaccgt gttcggtatt cccggtgtac acaacctgga
     3721 attgtatcga ggcctcgccg catcgaacat tcgccatgtg ctcgtgcgcc atgaacaagg
     3781 cgcggggttc gcggccgacg gttttgcgcg tttgagcggg cggcccgcgg ccgccttcgt
     3841 gatctccggg cccggtctca ccaatatcct gaccgcggtc gctcaagctt actcagattc
     3901 ggtgcccctg ttggtgatcg ccagcgcgcc cgtacgcgcc tcgttccgca agcagtgggg
     3961 cgtgctgcat gagttatacg accaggccgc gcttgccgcg ggcgtctttg gggtggcgcg
     4021 caccgctttc agtgccgaag atgtgcgcga tcatctgcga ttgtgcctcg cgtccttttg
     4081 caccggacgg ccgcgtccgg cgtacctcga aatcccgctc gacgtgctgg cagaaggcac
     4141 cacgttgcgg gcgcaacgat tcgagggcgg cacattgatg ccgcaagcaa gccccgagca
     4201 gatcgacgcg gcagtgcggg ttctggagct cgccgcgcga cctctggtca tcgccggcgg
     4261 cggcgcacgc catgccggcg cggagttgca tcgcctggtc gaaaccctgg attgcccgtt
     4321 ggtgacgacg gccgcgggca agggcgtgct cgcggagagt catccggcga acttcggtac
     4381 ctcgctgccc taccgttgca tccaacaact catcgtggat gcggatctgg tactcgcggt
     4441 cggtacggag ctcggcgaga ccgacatcta ctccgcgacc aaactgccgc tcggcggaat
     4501 gttggtgcgg atcgacgttg atccggtgaa gttgtccgac cagtactcgg ccgatgtctg
     4561 cgtttggtcc gatgcgcgca gcgccctgcg ggccatcaac catgccttgg cgaaccgctt
     4621 gcccgagagc gcggtgcgct cacggtggcg cacggcgctc ggcgatggcg cgaaaatccg
     4681 ctcggaaata gacaacggct tcgatgccaa ggcgcgggcg cagtggcacg cgctgcgcgc
     4741 catcaaggcg gctttgccgt gggacggagt cgtattcagc gacatgactc agattgccta
     4801 tttgggcaat tacgcctatc cggtcgacaa tccgggacaa tggttccatc catccggtta
     4861 cggcacgttg ggattcgcat tgccggcggc gctcggcgcc aagatcgcat gcccggcgcg
     4921 cgccgtcatt gcgctcgccg gcgacttcgg tctgcagttc acgctgccgg aaatcatgac
     4981 cgcgatcgag accggtatca gcctgcctat cgtcgtgtgg aacaactcgg cgctcggtca
     5041 aatccgcgac gacatggtcg cggcgggcat agcgcaactc ggagtcatcg cgcgcaatcc
     5101 ggacttcgtt tcgctggcgc gcgcctgcgg tgccgagggt tatcgggtgc acgacgcgaa
     5161 cgcgctcacc gaggcaatac gcaccgcctt aagtcatacg gggccgacct tgatcgaggt
     5221 ggtcgccacg agtttctcaa taacctgaag gggatgacat tgaaaatcac gagttgtctg
     5281 acggcgcttg ccgcaacgct tctgacgcat tcggccgcgg cagccgagca aatcaaagtc
     5341 gtcgagcacc cggtcggcga gaccacggtc gacctcggcg cgaaaggcga ttcgatcggc
     5401 gacctgctgg tattcgcgaa cggcgtattc gactccgcga acaaggttca gatcggatcc
     5461 gatcaaggct attgcgtgcg caccgtcgtc ggcaagagct gggaatgcat gtggacgctg
     5521 ctcttgaagg gaggtcaaat caccgtcgaa ggaccattca tggatgaggg tgattcgctg
     5581 ttcgccgtga ccggcggcac cggcaagtat gccggagcca agggcagcat gaaattgcat
     5641 gcgcgcgatg ccaaggcgtc gagctacgac ttcgtttacg acctcctgta atggcctaca
     5701 cgcacgagga ctgcctgcgc gcgcggcagg attttccggc gctgaggcgc gcgccgcatc
     5761 tcgcctacct cgacggacct gccggcagcc aggtgccgcg cgccgtcgtg gaggccatcg
     5821 cggacgttta cgcatcgtgc aatgtgaaca cgcgcggcaa cttcgcgccc tcgcgcgaag
     5881 tcgatcggcg cttggatgcc gcgcgcggcg cagtcgccgc ctttctcggc gcggagagcg
     5941 gcgcttgcat ttcatttggt caaaacatga ccacgctgaa cttcgccttg agcgccgcca
     6001 tcggccgcac tttgcacaag ggcgacgaag tgttgatcac gcagctcgac cacgaagcca
     6061 atcggggacc ttggatcggc ctcgaggaac gcggcgtggt ggtgcgcgaa gtgcgggtgc
     6121 tcgaatccgg cgaactggat tatgccgaca tggctgcgag gatatcgccg cgcaccaagc
     6181 tatttgcgct cggcgcatcg gcgaattcct tgggaaccgt gaacgatctc gcgctcgccc
     6241 ggcgcctgac gcacgaggtg ggcgccttgc tggtgattga cgccgtgcat tatgcgccgc
     6301 atttccccat cgacgttcag gcgctcggcg ccgatttttt gttgtgctcg gcctacaaat
     6361 tctacggacc gcatgtggga gtgttgtact cccgtcccgg cgcgctcgat cgcttgccga
     6421 ccaatcgcct gagcgttcaa gatccggcgg caccctaccg catcgaaacc ggcacactga
     6481 atcacgccgc gatcgacggc gtacgcgccg cggtggagta catcgcgggc tggggaagcg
     6541 gtgcgtcgtt gcgcgagcgt atcgtctccg cgatgacgca aatcgccgcc tatgaacacg
     6601 gcttggcaaa atcgtacttc gacgcggcgg cacgcatgcc cggcgttcgc gtatggggcc
     6661 cggattttac cgcgggccgc gcgcgcgcgc cgaccgtctc gatcacgctt gccaaaatca
     6721 ccgcagacgc tgccgcggga gtactcgggg agcaaggcat ttgcgtttgg gacggaaatt
     6781 tctacgcggc ccgtccggcg caaatgctcg gattcaccga gcgcggcggc ctgctgcgta
     6841 ccggtgtttc catgtacaac acccgcgatg aaattgatcg attgctgagc ggactcgaac
     6901 ggctgttatg aaccttgcgc tcgggggccg ggcatctcga ccagatgcga cgcgagttcc
     6961 cgagcgtgat cccgcagttc cgtcgcggct gtcgcctccc aatgattggc gcgcagcatg
     7021 ggacccaagt aataaaggcc ctcgctcggc acgccgtgca tgtcgatgca ggctccaaac
     7081 cgcccggtgc gcaatcctaa attgagcgca tcctccgata ccaatcctgc ctggtgcagg
     7141 gaactcaaca gcggattcgt cgatcgttcg agcccgtacc cgggcccggt cgcgttgacc
     7201 acacaatcca cgatgagcgt ggcgctggat cggccgccgc gaggccgcca gaacacgtgc
     7261 aaccgatccc cgtccgccgt taccgaatcg atgcggcctg cgttcacgcg caatttgccg
     7321 ctgctgcgca gcccgtcaat gcgcgcgccc atatccggcg gcatgcggtg acggtgaacg
     7381 tcccagtggg gctgcaggtg gcgcacgaag cgccgtcgct ccgcctgcgg taatcgcgac
     7441 cacaacgccg gtgccaattg gcgaatgaac gtgaccacct cacgccaatc gccgccgttc
     7501 tgttcagctt ggcgagtcca ctcgcgggcc atcgccagca cccgccgcaa cgagtgagca
     7561 cacgccagca tcgccacgcc gtcgccaggc gccgcactgg gtcgcaatgt cgcctgcggc
     7621 aagggcacca gaccgcgccg cgaaatcgta tgcagcagcg gcgcgcggct cggatcctcg
     7681 ctcaacgcca cagcggcatc cgccattgat agtccgttgc cgacgatcag caccgagtgg
     7741 gtgtgcgtca gccgcttcgg cagtgcccag ggatcgtgcc ggtaggcgtg gtgatcgcga
     7801 accgcggccg cccacggcgg cagcgacggc ggcggactcc cgaccgcaag aatcacgcga
     7861 tccgccgcta gcggtggtcg atcggcgaac tcgacggcga agggtttgtc gccgccgcga
     7921 cgagtgatgt gccgaacctc atcgcacagg cgcaccagcc gcacgtgcgc cggtgctgcg
     7981 cgttcagctt gcagcagcac gtctcgaatg taatcgccgt agagcgcacg cggcagaaaa
     8041 tcctcgccgt cggcgccggg catgtgccgc cgtgcgaagc gcagaaattg cagcggatcc
     8101 tgcgcatccg ccgacaacct cgccgcgggc acgttcaaca gatacgggta gtcgcgagcc
     8161 gcatacgcca cgccgcgccc gatatcgggg gcctgctcga tcagcacgac atcggtcgca
     8221 tacgatggcg gacggcgcag caaatttgcg gcgagcaccg tgccgctgaa gcctgcacca
     8281 acgatcgcca ttatattgcg cattcagaaa ttctaacagg tagctgttgc gacaccgcaa
     8341 atcggcgcgg ggtcgttgaa cttcgcgaat gtgcccacat ctaacttgcc agggtcaatt
     8401 cgacctgccc gggggcctgt tatgcgaaga ttaatcgttg ctgcaaccct tgccacggcc
     8461 gtgactttcg ccgtcacatt gccggcgttt gccgacaccg atccgaccgt cgaccagatt
     8521 tacgccgagg cgcgcagcgg tcatctggag caagcgcaac agatgatgaa ccaggtgttg
     8581 gcggatcatc ccaacagcgg ccgtgctcac tacgtgcagg cggaactgta tgcacgcgag
     8641 ggcaaaactg cgctcgcgcg ttcggagctt gccaccgccg aacagttgaa gcccggtctg
     8701 ccgttcgaaa gtcagcgctc ggtccaagaa ctcaaagcac aagtcgggct tggctcgcgc
     8761 cccgcgacgc cgatcggcat gcgctctgaa tccgtcgcgc cgcgatttcc cctcggtacg
     8821 gtcttgatcc tggtggccgc tgtcggtctt ctatggatgc tgtttcgccg ccgcagctac
     8881 agccagtacc ccgcagccgg tcctggcgtg ggcggtacac ccggctatgg acctgggccg
     8941 ggatatggcc ctgctccgat gggtggcggc ggaatcggat ccgggattgc gggcggcctt
     9001 gcctcaggtc tcgcggttgg cgcgggcgtc gtagccggcg aagaactcgc gcatcatttc
     9061 ttggacggcg gtcgcggcgg tggtgtcatt ccccctgcgg aggccggcga ttccggtgtc
     9121 agcaatagcg acatgggcgg ctccgacttc ggcatgagcg atcccggcgc gggttgggat
     9181 gacggcggcg gatccgacgg cggcggcggc ggcggtgatt ggagctagag cgcgggccgc
     9241 caatcttccg gtgagcggtg cctgctattt aacataatgt agtcggctct agtggctggc
     9301 gcgcgccacc gccgtcgcga ccgcatcgtg cagcgccttc atgccgaagg agatgtcctg
     9361 cacgacaccg tcgatgaaga aagtgggcgt cgcgcgaatg tggctgcggc gcgcaccttc
     9421 aatgtgctca cgcaccttct gtagatagat gtgatcgtcc atctccgcgg tgtagcgggc
     9481 catatcgagc cccagttcgg cggcgtgacg gtacagatcc ttctccttga ggtgcatttg
     9541 gttctggaac agcaggtcat gcatctgcca aaactttcct tgcgcggcag cggcctccgc
     9601 aacctcggcc gccatcagcg catgcggatg tgcatcctgc accggaaaat gtcgaaatat
     9661 gaagcgcacc ttgttcgggt accgctccaa cagcagcttc ggcgtcgtcg ccgcgacttt
     9721 gcaactcg
//
LOCUS       DASWZT010000039         2702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_371, whole genome shotgun sequence.
ACCESSION   DASWZT010000039 DASWZT010000000
VERSION     DASWZT010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1475)
                     /locus_tag="VGE92_01015"
     CDS             complement(105..1475)
                     /locus_tag="VGE92_01015"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEY1312420.1"
                     /translation="MDTDREKNRFSARAKRYANVGTKVGGVAARIAGQRLVGATGGRA
                     GNALALAAALGGLKGPIMKVAQLLATIPDALPPEYANELIKLQSQAPPMGWAFVKRRM
                     NAELGADWQTKFNEFEHHPAAAASLGQVHRATALEGSRLACKLQYPDMQSAVEADLGQ
                     LNLLMSLRKRFDPAIDASEIAVEIGARLREELDYQREAKHIALYRNMLKDENAIRVPQ
                     VWPELSTGRLLTMDWMTGRKLLDHKDASLAVRNQLATAMFTAWWFPFSRFGVIHGDPH
                     LGNYTVFDDAKGKPAGINLLDYGCIRIFPPSFVGGVVDLYRGLLEGDDALVVHAYETW
                     GFKRLNRELIDALNIWARFIYGPLLDDRVRSIADGVKPSQYGRKEAFQVHQALKKLGS
                     VTVPREFVFMDRAAIGLGAVFLHLAAELNFYRLFNEAIDSFSARAVAARQQAALDKVG
                     LKPSTT"
     gene            1534..2049
                     /locus_tag="VGE92_01020"
     CDS             1534..2049
                     /locus_tag="VGE92_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312421.1"
                     /translation="MPVRFIVCLCALLAATNGASAAILSWGCMGQLDDQRVLFNRDGI
                     YIVDGKAPASKPKKFTADSVSDAIAELKKSKDTFTEFEPYGDDGLEGPIAFSTVDANK
                     QTQKLVFTQKSSHLISHKHRMICGRDEDSDLYRTVYSFERPCQPAQDIKMQCAYYQLS
                     TRGGRKGCNDD"
     gene            complement(2046..>2702)
                     /locus_tag="VGE92_01025"
     CDS             complement(2046..>2702)
                     /locus_tag="VGE92_01025"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY1312422.1"
                     /translation="PRDVMTLAHELGHGVHQVLAAKNGALMAPTPLTLAETASVFGEM
                     LTFKKLLAQTTDKKQRKAMLAAKVEDMINTVVRQIAFYSFERKVHLERRNGELTSDKI
                     CELWMSVQAESLGPAIELKAGYETFWSYIPHFIHSPFYVYAYAFGDCLVNSLYAVYEK
                     SASGFAERYLAMLAAGGTKHHTELLAPFGLNARDPKFWDGGLSVIAGLIDELEALEAR
                     "
BASE COUNT          505 a          839 c          862 g          496 t
ORIGIN      
        1 ggcgtattcg acggtgccgt ggtcggccat ggggggaggt ctccggaatg ccctgcagca
       61 gaggggttag cgtacttggc cgttcagggc aacgcccgca cgacttaagt agtggacggt
      121 ttgaggccca ctttatcgag cgcggcttgc tggcgcgccg ccaccgcgcg cgccgagaag
      181 gagtcgatcg cctcgttgaa caaccggtag aaattcagct cggccgccag atgcaggaac
      241 accgcgccca gcccgatggc cgcgcggtcc atgaacacga attcgcgcgg cactgtcacc
      301 gagccgagct ttttcagcgc ctggtgcacc tggaaagctt ccttgcgacc gtattgtgac
      361 ggcttgacgc cgtccgcgat gctgcgcacc cggtcgtcga gcagcgggcc gtagatgaag
      421 cgagcccaga tattgagcgc gtcgatcagc tcgcggttga gccgcttgaa gccccaggtc
      481 tcgtaggcgt gcacgaccag cgcgtcgtcg ccctcgagca ggccgcgata gagatcgacc
      541 acgccgccga cgaagctggg cggaaagatg cggatgcagc catagtcgag cagattgata
      601 ccggcaggct tgcccttggc gtcgtcgaat accgtgtaat tgccgagatg cgggtcgccg
      661 tggatcacgc caaagcgact gaacggaaac caccaggcgg tgaacatggc ggtggccagt
      721 tggttgcgca cggcaagcga ggcgtccttg tgatcgagca gcttgcggcc ggtcatccaa
      781 tccatggtca gcagacggcc ggtcgagagt tcgggccaga cttgcggtac gcgaatggcg
      841 ttctcgtcct tgagcatatt gcgatagagc gcgatgtgtt tggcctcacg ctgatagtcg
      901 agctcctcgc gcaggcgcgc gccgatttcg accgcgatct cgctggcgtc gatggccggg
      961 tcgaatcgct tgcgcagcga catcaacaga ttcaactggc ccaaatcggc ctcgaccgcc
     1021 gactgcatgt ccgggtattg cagcttgcag gcaagcctcg aaccctcgag cgccgtagcg
     1081 cgatgcacct ggccgagcga ggcggccgcc gccggatgat gctcgaattc attgaacttg
     1141 gtttgccagt cggcgccaag ctcggcgttc attcggcgct tgacgaaagc ccagcccatc
     1201 ggcggcgcct ggctctgcag cttgatcaac tcgttggcgt attccggcgg cagcgcgtcg
     1261 ggaatggtgg cgagcagttg cgccaccttc atgatcgggc ctttgagccc gccgagcgcg
     1321 gcggcgagcg ccagcgcgtt gccggcccgg ccgccggtcg cgccgaccag acgctgcccc
     1381 gcgatgcgcg cggcgacgcc gccgactttg gtgccgacat tggcgtagcg cttggcgcgg
     1441 gcggagaagc ggtttttttc gcggtcggtg tccatgaacc cccggggtac cagtgtgcta
     1501 cgaatatagg aagcagccag ggagaaacga gccatgcccg tgcgctttat cgtctgcctt
     1561 tgcgccctgc tcgcggccac gaacggcgcc tccgccgcga tcttgtcatg gggctgtatg
     1621 ggccaactcg acgaccagcg cgtgctgttt aaccgcgatg ggatttatat cgtggacggc
     1681 aaggcgccgg cttccaagcc gaagaaattt accgccgatt cggtcagcga cgcgatcgcg
     1741 gagctgaaga aaagcaaaga tacgttcacg gaattcgaac cctatggcga cgacggattg
     1801 gaaggcccga tcgccttctc aaccgtcgac gccaataagc agacgcaaaa actcgtgttc
     1861 acgcaaaagt cgtcgcatct gatctcgcac aagcaccgca tgatttgcgg acgcgacgag
     1921 gattccgacc tctaccgcac ggtttatagc ttcgagcgcc cctgtcagcc ggcgcaggac
     1981 atcaagatgc aatgcgcgta ctatcagctc tcgacccgtg gcggacgcaa gggctgcaac
     2041 gacgactagc gcgcctcgag cgcttccagt tcgtcgatca gcccggcgat cacgctcagg
     2101 ccgccatccc agaatttcgg atcgcgcgcg ttgaggccga acggcgctag cagctcggtg
     2161 tgatgcttgg tgccgccggc cgccaacatc gccagataac gttcggcgaa gccgctcgcc
     2221 gatttctcat agaccgcata gagcgaattc accaggcaat cgccgaacgc ataggcatag
     2281 acataaaacg gcgaatggat gaaatgcggg atgtagctcc agaacgtctc gtaaccggct
     2341 ttcaactcga tcgcggggcc gaggctttcg gcctgcaccg acatccatag ctcgcagatc
     2401 ttgtccgacg tcagctcgcc gttgcggcgc tccagatgca ccttgcgctc gaacgagtag
     2461 aacgcaatct ggcgcaccac ggtgttgatc atgtcctcga ccttggcggc gagcatggcc
     2521 ttgcgctgct tcttgtcggt ggtctgcgcc aggagcttct tgaaggtgag catctcgccg
     2581 aacacgctcg cggtttcggc gagcgtgagc ggcgtcggcg ccatcagcgc gccgttcttt
     2641 gccgccagca cctgatgcac gccatggccg agttcatggg cgagtgtcat cacgtcgcgc
     2701 gg
//
LOCUS       DASWZT010000040         1863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_58439, whole genome shotgun sequence.
ACCESSION   DASWZT010000040 DASWZT010000000
VERSION     DASWZT010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58439"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..669)
                     /locus_tag="VGE92_01030"
     CDS             complement(82..669)
                     /locus_tag="VGE92_01030"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY1312423.1"
                     /translation="MSAATSANNYEYLDILDRSWSESGLERPAGGVLCDIGCASFWYA
                     ATLQAFFRPARLVGVEVEGYRLFRDGHTRIDYASGYLKQLPNAEFVVADYTRYPQPAG
                     LITAWFPFLTPAALLAWRLPLRLLRPDKLFERIRHNLEPDGTFFMVNHGMQEWALAER
                     LCTAAGLRLVSHWSEPSTLSAHRLEPPAVSWWRHE"
     gene            866..1816
                     /gene="lpxL"
                     /locus_tag="VGE92_01035"
     CDS             866..1816
                     /gene="lpxL"
                     /locus_tag="VGE92_01035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferase"
                     /protein_id="HEY1312424.1"
                     /translation="MLRVELYRPKYWATWVGLGVLRSIELLPFGWQLHVGSAIGRLIR
                     RLPLAYVRIARRNIELCMPALRPREQADLLNRHCRSLGMALCETANTWWSSDRRVNRL
                     ADVQGLEHLQAAFAKGRGAIMIGGHFTTIEIATRILGTVVPLNVVYRPTKNAVLSHTM
                     FSSFSRHGKPIAHDDIRTMIRALKNNEAVWYAPDQSYRNKGAAMVKFFGIPAATTTAT
                     SRLARISGAAVLTYFPERLPGAAGYRVVIGPAFENFPGADPICDVERFNRLLEAQIRR
                     VPEQYLWVHRRFKGLGADYPDYYGRDSRKRTPIPTAKTTG"
BASE COUNT          372 a          612 c          552 g          327 t
ORIGIN      
        1 tgaccagaaa cggaccaagt agaaattcac gagccgccgc cgatattaca gcaaattact
       61 gattgcttcg aatatttctg tctattcatg ccgccaccag gacactgcgg gaggctccaa
      121 ccgatgcgca ctcaaggtcg aaggctccga ccagtgcgac accaagcgca accccgctgc
      181 agtgcacaat cgctccgcca gcgcccattc ctgcatgcca tggttcacca tgaagaacgt
      241 gccgtcgggc tcgagattat gtcgaatccg ctcgaacagc ttgtcgggcc gcagcaacct
      301 taagggcaaa cgccaggcca gcagggccgc aggcgtcaga aacggaaacc aggcggtgat
      361 gagcccggcc ggctgcgggt agcgggtgta atcggcgacc acgaactcgg cgttcggcag
      421 ctgtttcaga tagcccgacg cataatcgat ccgcgtgtgt ccatcgcgaa acagccggta
      481 accctccact tcgacgccga ccagccgcgc cggccggaag aaggcttgca gcgtcgccgc
      541 gtaccagaaa cttgcgcaac cgatgtcgca gagcacgccg cccgccggcc gctcgagacc
      601 cgactcgctc cagctgcgat cgagtatgtc caagtattcg tagttgttgg cgctcgtcgc
      661 cgcgctcatg caacgctcaa attgcacctg gtaacgcccg cgcaacgcag ctattctctc
      721 gccctgctcg aggtctacgc tgcaaagttc gcttgccggt gtctcctcaa gcgcaccgcg
      781 cgaccaacgg atgcgctcgg agaggccgaa ccagaaacca tgtcgcaggg agcttagtcg
      841 ctgaagcaat gaatacatcg gccaaatgct acgcgtagag ctctatcggc ccaagtattg
      901 ggcaacgtgg gtgggcctcg gcgtattgcg cagcatcgaa ttgctgccgt tcggttggca
      961 actgcatgtc ggctcggcga tcggccggct catccggcgg ctgccgctcg cctacgtgcg
     1021 catcgcgcgc cgcaatattg aactgtgcat gcccgccttg cggccgcgcg agcaggccga
     1081 cttgctcaac cgtcattgcc gaagcctcgg gatggcattg tgcgagacgg ccaacacctg
     1141 gtggagcagc gaccggcgag tcaaccggct tgccgacgtc cagggcctcg aacatctgca
     1201 ggcggcgttt gccaaaggcc gcggcgccat catgatcggc ggccacttca ccaccatcga
     1261 aattgcgacg cgcattctgg gtacggtggt gccgctcaac gtcgtttatc gcccgaccaa
     1321 gaatgcagta ctgtcccata ccatgttcag cagcttctcg cgccacggca aaccgatcgc
     1381 gcacgacgat atccgcacga tgattcgcgc actgaagaac aatgaggccg tgtggtatgc
     1441 gccggaccag agttatcgca acaagggcgc ggcaatggtg aaatttttcg ggatcccggc
     1501 cgcgaccacc accgccacct cgcgtctcgc gcgcatttcc ggcgcggcgg tcctgaccta
     1561 ctttccggag cggctgccgg gcgcggccgg ctatcgggtc gtgatcggac cggcattcga
     1621 aaattttccc ggggcggatc ccatctgcga cgtcgaacgc ttcaatcgct tactggaggc
     1681 gcagattcgc cgcgtgccgg agcaatatct gtgggtgcac cgccgcttca aggggctcgg
     1741 cgccgactat cccgactact acggccgcga ttcgcgcaag cgcacgccga tacccacggc
     1801 aaagaccacc ggctaacggc gagtccgcgt caggggctgg cggatgcgtc ggccttctcg
     1861 agc
//
LOCUS       DASWZT010000041         5914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_19742, whole genome shotgun sequence.
ACCESSION   DASWZT010000041 DASWZT010000000
VERSION     DASWZT010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19742"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /gene="truD"
                     /locus_tag="VGE92_01040"
     CDS             complement(<1..396)
                     /gene="truD"
                     /locus_tag="VGE92_01040"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HEY1312425.1"
                     /translation="MSRESWVRAALQPPRAHGLPLPPAELKSVPEDFHVEEQLSFAPS
                     GSGPHLLLQVEKRTANTHWVAKQIAQLARVAAADVGYAGLKDRHAVAVQWFSVPALAS
                     SADFWSDVSTPEFRVRQVLANSRKLRRGAL"
     gene            complement(393..1595)
                     /gene="ispD"
                     /locus_tag="VGE92_01045"
     CDS             complement(393..1595)
                     /gene="ispD"
                     /locus_tag="VGE92_01045"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEY1312426.1"
                     /translation="MTAAHAARRVWAIVPAAGRGARFSPSAAASPPKQYAPLLGATVL
                     EWSLQALLAEPRVHAVVVALAADDTHWPGVAAKLNQSKLQTTVGGANRRDSVMNGLEL
                     LLTQAAPDDWILVHDAARPCFTAIELRGLLAALDRPQNADGAVLAAAIVDTVKRERGD
                     QVATVDRQGLWRALTPQVFAFQQLRRALQESVRAGVEVTDEAQAIEALGLRPALVQGS
                     PFNIKVTHAEDLSIAERILKMTQCSAMRIGQGFDVHAFGTGDHVMLGGVRIAHSRGVV
                     AHSDGDVVIHALCDALLGALGDGDIGQHFPDSDSRYRGVDSRVLLRAVAQRVQAAGYR
                     LVNADITVLAEAPRVAAHRSAMAANLGADLGMEPKLVSIKATTTERLGFIGRGEGLAA
                     MASVLLTT"
     gene            complement(1592..1915)
                     /gene="ftsB"
                     /locus_tag="VGE92_01050"
     CDS             complement(1592..1915)
                     /gene="ftsB"
                     /locus_tag="VGE92_01050"
                     /inference="COORDINATES: protein motif:HMM:NF002058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEY1312427.1"
                     /translation="MRILATVLALALVSLQYRLWLSDQGLREVSRLQSAIDAQAAANR
                     EQGERNRQLVAEVTDLKVGLTALEERARSDLGMVGNSETFYQVVTATTPAPAAPATGV
                     TARTE"
     gene            complement(1941..3227)
                     /gene="eno"
                     /locus_tag="VGE92_01055"
     CDS             complement(1941..3227)
                     /gene="eno"
                     /locus_tag="VGE92_01055"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEY1312428.1"
                     /translation="MSRIVEIRGRQIIDSRGNPTVEADVVLHSGVVGRAAVPSGASTG
                     SREAVELRDGDKKKYGGKGVLTAVAHVNGALRDLLVGYDAQDQRGLDARMIKADGTDF
                     KSNLGANALLAVSLAAAHAAARDKGVPLYRHLAGSGDLTLPVPMMNIINGGAHADNSI
                     DIQEFMILPIGAPSFSEAVRYGAEVFHSLKKVLHAAGLNTAVGDEGGFAPDLPSNEAA
                     LTTILEAIDRAGYRAGKDIYLGLDVASSEFFKDGVYTLESEGRHFTAAEFTDYLANLA
                     GKYPIVTIEDGMSEGDWDGWGILTKKLGSKLQLVGDDLFVTNTKIFAEGIAKKIANAI
                     LIKPNQIGTLTETLAAIDMAHAAQYAAVVSHRSGETEDSTIADIAAATTATQIKTGSM
                     SRSDRIAKYNQLLRIEGELGSKAGYAGRRALSVPIS"
     gene            complement(3224..4057)
                     /gene="kdsA"
                     /locus_tag="VGE92_01060"
     CDS             complement(3224..4057)
                     /gene="kdsA"
                     /locus_tag="VGE92_01060"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEY1312429.1"
                     /translation="MKLAAFEVGAEQPFFLIAGPCVIESEALVIDVAGRLKEMTGRLA
                     IPFIFKASFDKANRSSRSSFRGPGVEGGLKVLARVREQIKVPVLTDVHEDTPLAEVAA
                     VVDVLQTPAFLCRQTNFIVAVASQGKPVNIKKGQFLSPWEMQNVVDKARSTGNLEIMV
                     CERGFSFGYNNLVADMRSLAIMRGTGCPVVFDATHSVQLPGGKGDVSGGQREFVPVLA
                     RAAVAAGVAGVFMETHPDPAKALSDGPNAWPLKRMESLLGTLKELDRSVKKQPFEEDL
                     L"
     gene            complement(4054..5730)
                     /locus_tag="VGE92_01065"
     CDS             complement(4054..5730)
                     /locus_tag="VGE92_01065"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004815797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY1312430.1"
                     /translation="MRTDLIGGDHMTRFVFVTGGVVSSLGKGIASASLGAILEARGLK
                     ISMVKLDPYINVDPGTMSPFQHGEVFVTADGTEADLDLGHYERFVRMVAGRNSNFTTG
                     RIYERVIAKERRGDYLGATVQVIPHITDEIKYRVRMGAGDADVCMVEVGGTVGDIESL
                     PFLEAIRQMGVELGRNRVLYMHLTLVPIVGGSGEIKTKPTQHSVKELRGIGIQPDILL
                     CRCAHSLPDEQRRKIALFTNVEERAVISGVDSDDIYKIPMLLHEEGLDDIVVDKLRLD
                     APPTDLPEWRAVVAAKQNPEAVIDIGMVGKYVQIRDSYISLNEALMHGGIKTRTRVNI
                     HYFESQDIERLGPAALQNMDAILVPGGFGDRGIEGKIQAIRFARENRIPYLGICLGMQ
                     LAIIEYARNVLALKGANSTEFDRATHHPVIALITEWQDLERGQQVRDEKSDLGGSMRL
                     GAQEAKLVPGSLAHALYGKESIFERHRHRYEFNNHYLAELAAAGLSFSGFSADGLVEF
                     IEVRAHPWFVASQFHPEFTSTPRDGHPLFTGFVRAAREYRAALQPGRATA"
BASE COUNT         1121 a         1914 c         1766 g         1113 t
ORIGIN      
        1 gagcgcgccg cgccgcagct tgcgcgagtt cgcgagtacc tggcgcaccc tgaattccgg
       61 ggtgctcacg tcgctccaga aatcggcact gctcgccaaa gcgggcacgc tgaaccattg
      121 aacggcgacc gcgtgccggt ccttcaagcc tgcgtagccg acgtccgccg cagcaacgcg
      181 ggcgagctgt gcaatctgct ttgcgaccca atgcgtgttg gcggtgcgct tttcaacctg
      241 cagcagcaag tggggaccgg aacccgaggg cgcgaatgac aactgttcct cgacgtggaa
      301 gtcttccggg acggatttca actcggccgg cggcagcggc aacccgtggg ctcgcggtgg
      361 ctgcagcgcc gcccgcaccc acgactcgcg gctcacgtgg tgagaagcac tgaggccatt
      421 gccgccaacc cttctccgcg accgataaaa ccgaggcgtt cggtcgtcgt tgccttgatg
      481 cttaccaact tgggctccat gccaagatcg gcgccgagat tcgccgccat cgccgagcgg
      541 tgtgcggcaa cgcgtggcgc ctccgcgagt accgtgatat ccgcgttcac caggcgatat
      601 cccgcggctt gcacgcgctg cgccacggct cgcagcaaca cccggctgtc gaccccgcga
      661 tagcgcgaat cgctgtcggg gaagtgctgg cctatatcgc cgtcaccgag cgcacccagc
      721 aaggcgtcgc aaagcgcgtg aatcacgaca tcgccgtccg aatgggccac cactccgcgg
      781 ctgtgtgcta tgcgcacccc gccgagcatc acgtgatcgc cggtgccgaa tgcatgcacg
      841 tcgaagcctt ggccgattct catcgcactg cattgcgtca ttttcaagat cctctcggcg
      901 atgctcaaat cctctgcatg cgtcactttg atgttgaacg gtgaaccctg cacgagagcc
      961 ggccgcaacc ccaaggcctc gatcgcctgc gcctcatcgg tcacctctac tcctgcacgc
     1021 accgattctt ggagtgcgcg gcgcagctgc tggaatgcga atacctgcgg cgtcagcgcg
     1081 cgccacaatc cctgccgatc cacggtcgct acctgatcgc cgcgttcgcg cttcacggta
     1141 tccacgatag ccgcggcgag caccgcgcca tccgcgttct gcggccgatc gagggccgcc
     1201 agcaagcctc gcaactcgat cgcagtaaaa cacgggcgcg ccgcatcgtg caccaagatc
     1261 cagtcgtccg gggccgcttg cgtcagcagc agctcgaggc cattcatcac cgaatcgcgc
     1321 cggttcgcac cgccgacggt ggtttgcagt ttcgactgat tcaatttggc cgcgacgccc
     1381 ggccagtgag tgtcatccgc ggcgagcgca accacgacgg cgtgcacgcg cggctccgct
     1441 aacagcgcct gcagcgacca ttccaagacc gtggcgccca gcagcggcgc atattgtttg
     1501 ggaggcgagg ccgctgcgga aggactgaag cgggcaccgc ggcccgccgc aggaacgatc
     1561 gcccacacgc gccgcgccgc gtgagccgcg gtcattccgt tcgcgcggtg acgcccgtgg
     1621 cgggtgcggc gggcgctggc gttgtggcgg tgacgacttg gtagaaagtc tcgctgttgc
     1681 cgaccatgcc gagatcgctg cgggcacgct cctcgagcgc ggtcaggccc actttgagat
     1741 cggtcacttc ggcaaccaac tggcgattgc gttcaccttg ctcgcgattg gctgcggcct
     1801 gcgcgtcgat cgcgctttgc aggcgcgaga cttcacgcaa tccctggtcc gagagccaca
     1861 accggtactg cagcgagacc agggccagag ccaggacggt ggctaaaatc ctcatgggga
     1921 caggagctta gcatgaactc ttaagaaatt ggtaccgata aagcgcgccg gcccgcgtag
     1981 ccggccttgc tcccaagctc gccctcgatg cgcagcagtt ggttgtattt ggcgatacgg
     2041 tcggagcgcg acatcgagcc cgtcttgatc tgcgtggccg tggtcgcggc ggcgatgtcg
     2101 gcaatggtcg agtcctcggt ttcgcccgag cggtgcgata ccaccgcggc atattgggcc
     2161 gcgtgcgcca tatcgatggc cgccaatgtc tcggtcaatg taccgatctg attcggcttg
     2221 atgagaattg cattggcaat ctttttcgcg atgccctctg cgaaaatctt cgtattggtc
     2281 acgaacagat catcgcccac cagctgcagt ttggatccca acttcttggt gagtatcccc
     2341 caaccgtccc agtcaccttc gctcatgcca tcctcgatgg tcacgatcgg atatttgccg
     2401 gcgagattcg ccaggtaatc ggtgaactcc gccgcggtaa aatgccggcc ctcggattcg
     2461 agcgtataga caccgtcctt gaagaactcc gaactggcca catcgaggcc gagatagatg
     2521 tccttgccgg cgcggtaacc cgcccggtcg atggcttcca atatggtcgt cagggcagcc
     2581 tcattcgacg gtaaatcagg tgcgaagccg ccttcatcac cgaccgccgt gttgagtccc
     2641 gcagcatgca gcactttttt caggctgtga aagacttcgg cgccgtaacg cacggcctcc
     2701 gagaatgacg gcgcgccgat cggtaatatc atgaattcct gaatgtcgat gctgttgtcg
     2761 gcgtgtgcgc cgccgttgat gatgttcatc atcggcacgg gcagcgtgag atcgccggag
     2821 ccggccaggt gccggtatag cgggacgccc ttatcgcgcg ccgccgcatg cgccgccgcc
     2881 aacgacacgg ccaacagggc gttcgccccc agattggact tgaagtccgt gccgtcggct
     2941 ttgatcattc ttgcatcgag gccgcgttgg tcctgggcgt cgtagcccac gagcaaatcg
     3001 cgcagcgcgc cgttgacatg cgcgactgct gtgagcacgc ccttgccgcc atattttttt
     3061 ttgtcgccgt cgcgcaattc caccgcctcg cgcgatccgg tcgacgcgcc ggatggcacg
     3121 gccgcccggc cgacgacgcc ggagtgcagg accacgtcgg cttcgacggt gggattgccg
     3181 cgcgagtcga tgatctggcg gccgcgaatc tcgacgatac gactcatagc aggtcttcct
     3241 cgaagggttg ctttttcacc gagcgatcca attccttgag cgtgcccaag agtgattcca
     3301 ttcgcttcag gggccaggcg ttgggcccat ccgagagcgc cttcgccgga tcgggatgcg
     3361 tttccatgaa gaccccggcg acgcccgcgg cgaccgcggc gcgcgccaac accggaacga
     3421 actcgcgctg tccgcccgac acatcgcctt tgccgccggg cagttgcacc gagtgcgtgg
     3481 cgtcaaacac caccgggcac ccggtgccgc gcatgatggc gagcgagcgc atgtcggcaa
     3541 ccagattatt gtagccgaag gagaatccgc gttcgcacac catgatctca agattgccgg
     3601 tggaacgagc cttgtcgacc acgttctgca tctcccaagg agagaggaac tggccttttt
     3661 tgatgttcac cggcttaccc tgcgatgcga cagcgacgat gaaattggtt tgccggcaca
     3721 gaaacgccgg cgtctgcagg acatccacga ccgcggccac ttccgcgagc ggtgtgtctt
     3781 catgcacatc ggtcaacacc ggaactttga tctgctcgcg cacccgggcc agcaccttca
     3841 agccaccctc gactccggga ccgcggaagc tcgagcggga ggaacggttt gccttatcga
     3901 acgacgcctt gaaaatgaac gggatagcga gacggccggt catctccttc aaacgccccg
     3961 ccacatcgat gaccagggct tcgctttcga tgacgcaagg accggcgatc aagaaaaacg
     4021 gttgctccgc gccgacctcg aaggcggcca gtttcatgcg gtcgcgcgtc cgggctgcaa
     4081 tgcggcgcga tattcgcgcg ccgcccgcac gaaccccgtg aacagcggat gtccatcgcg
     4141 cggcgtagag gtgaattccg ggtgaaactg gctggcgacg aaccaagggt gggcgcgcac
     4201 ttcgatgaac tccaccagac cgtccgccga aaaacccgaa aagctcaaac cggccgccgc
     4261 caattcggcg agatagtgat tgttgaattc atagcggtgg cggtgcctct cgaatatcga
     4321 ttccttgccg tacagcgcat gcgccagcga gcccggtacg agcttggcct cctgcgcgcc
     4381 gagccgcatg ctgccgccga gatcggattt ctcatcgcgc acctgctgac cgcgctccag
     4441 atcctgccat tcggtgatca gagcgatcac ggggtgatgg gtcgcgcgat cgaactcggt
     4501 gctgttcgcg cccttcaatg ccagcacatt gcgcgcgtat tcgatgatgg cgagttgcat
     4561 cccgagacag atgccgagat acggaatgcg gttttcgcgc gcaaaccgga tcgcttgaat
     4621 cttgccctcg atgccgcggt cgccgaaccc gcccggcacc agaatcgcat ccatattctg
     4681 caacgccgcg ggccccaggc gctcgatgtc ctgggactcg aaataatgaa tgttgacccg
     4741 cgtgcgcgtc ttgatgccgc catgcatcag ggcttcattc aatgaaatgt acgagtcgcg
     4801 aatttgtaca tacttgccga ccataccgat gtcgatcacg gcctcgggat tttgcttcgc
     4861 cgcaaccact gcgcgccact ccggcagatc cgtgggcggc gcgtccaggc gcagcttatc
     4921 gaccacgatg tcgtccagcc cctcttcgtg cagcagcatc ggaattttgt agatgtcgtc
     4981 tgagtcgacg ccggaaatca cggcgcgctc ttcgacattc gtaaacagcg cgatcttgcg
     5041 gcgctgttca tccggcagcg aatgcgcgca gcggcataac agaatgtcgg gctggatgcc
     5101 gataccgcgc agttctttga ccgaatgctg cgtgggtttg gttttgatct cgccggatcc
     5161 tccgacgatc ggcaccaatg tgaggtgcat gtaaagtacg cggttacgcc cgagctcgac
     5221 gcccatttgg cggatcgctt cgagaaacgg cagggattcg atgtcaccga ccgtgccgcc
     5281 gacctcgacc atgcacacat ccgcatcgcc cgcgcccatg cgcacgcggt atttgatctc
     5341 atcggtgatg tgcgggatta cctgaaccgt cgcacccagg taatcgccgc gccgctcctt
     5401 ggcaatgact cgctcgtaga ttcgccccgt cgtgaaatta ctgttgcgtc cggcaaccat
     5461 gcgtacaaag cgctcgtagt gaccgagatc gaggtccgct tcggtcccat cggcggtcac
     5521 gaacacctcg ccgtgttgaa atgggctcat ggtgcccgga tccacattga tatagggatc
     5581 aagcttgacc atgctgattt tcaggccgcg cgcctcgaga attgcgccga gcgaagcgga
     5641 cgcaatgccc ttaccgagcg aggaaacgac gccgccggtg acgaatacga atcgagtcat
     5701 gtggtcaccg ccgataaggt cagttctcac gcgttcacat cgcgagagca caaaccaaag
     5761 ccaaaaagga acaatgggaa ttcgacggga attcaggtta gcagaacatg ggccttgcga
     5821 caatcaactt aaccaataat cggcgcacgc tcccatacga tcgctgcacc cggctgcgag
     5881 ggagccgcgc accaacgcgc atccagccac aagt
//
LOCUS       DASWZT010000042         1780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_92342, whole genome shotgun sequence.
ACCESSION   DASWZT010000042 DASWZT010000000
VERSION     DASWZT010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92342"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1780)
                     /locus_tag="VGE92_01070"
     CDS             complement(<1..>1780)
                     /locus_tag="VGE92_01070"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEY1312431.1"
                     /translation="RIRVRETVSDSILDRADEVELVDLTPEDLIQRLGEGKVYVPAQA
                     ERAVHNYFKPGNLTALRELALRRTAQRVDEQMVNYMRAHAISGPWEASERVLVLVSER
                     PGAIALVRYARRLADRLRASWAAINIETGHTQRLSEAERDRIAEALRVAERLGGVAVS
                     VPAENVAAGIIDYAHANNFTHIVVSTSRRARWWELIRPSAAHEIIRRAGDISVHVVPE
                     DRSTKAATEEKPERTSVAPRPSSDWLMPSAYAGSLGFVAVALAVGLVLQQFLTVSSIA
                     LVFLTAVLVSAITYGRWPALFTCLISVLAYNFFFLPPLYTFTIADPENVVALFFFALV
                     AVIASNLTARVRAQAVTAQERAKVTEDLYLFSRKLTGVVTLDDLLWATAHQIALMLKL
                     RVVLLLPEGDGIAVRAGYPPEDSLDDADLAAARWSWEHALPAGRGADTLPGAKRLFLP
                     IRTGRGTVGIIGLDSDRPGPLLSPDQRRLLDALADQAALAIERVNLAQDIDRAKLTTE
                     TERLRTALLTSISHDLRTPLASILGSATSLRTYRKSLDDDAQDELIQTIQSESERLNR
                     FIANLLDMTRLESGAIMPKTEVTDLP"
BASE COUNT          338 a          565 c          574 g          303 t
ORIGIN      
        1 tccggaagat ccgttacttc ggttttcggc atgatcgcgc ccgattcgag ccgcgtcatg
       61 tccagcagat tggcgatgaa gcgattgagc cgttcggact cggactggat ggtctggatc
      121 aattcgtcct gcgcgtcgtc atcaaggctc ttgcgatagg tgcgaagact cgtcgccgag
      181 ccgaggatcg aggcaagtgg cgtgcgcaga tcgtgcgaaa tcgaggtgag aagcgctgtg
      241 cgcaggcgtt cggtttccgt cgtgagcttt gcgcggtcga tatcctgcgc gagattcacc
      301 cgttcgattg ccaaggcagc ctgatcggcc agtgcgtcga gcaggcgccg ctggtccggc
      361 gagagaagcg gacccggcct gtcgctgtcg aggccgatga ttccgaccgt accccggccc
      421 gtgcggatgg gcaggaaaag tcgcttggcg cccggcaacg tgtccgcccc gcggccggcc
      481 ggcagtgcat gctcccacga ccacctggca gccgcgaggt cggcgtcgtc cagcgaatcc
      541 tccggcggat agccggcgcg aaccgcgata ccgtcaccct cgggcaaaag cagcacgacg
      601 cgcaatttga gcatcaacgc gatctggtgc gccgtcgccc acaggagatc gtcgagtgta
      661 acgacgcccg taagcttgcg actgaacaga tagagatctt ccgtcacttt cgcgcgttcc
      721 tgcgccgtga cggcctgcgc gcgcacgcgc gccgtcagat tgctggcgat gactgcaacc
      781 aatgcaaaga agaacagcgc gacgacattt tccggatcgg caatcgtgaa tgtgtaaagc
      841 ggcggcagaa agaaaaaatt ataggccagc acgctgatca aacatgtgaa cagggcaggc
      901 cagcgtccat aggtgatcgc gctcaccaga acggccgtca aaaacaccag cgcgatgctc
      961 gaaacggtga ggaactgctg caacacgagg ccgaccgcca acgccaccgc gacgaagccg
     1021 aggcttccgg cataggcgct cggcatcagc caatcgctcg acgggcgtgg ggctacggac
     1081 gtgcgctccg gcttttcctc cgtcgccgcc tttgtcgagc gatcctccgg cacgacatgg
     1141 acgctgatat cgcccgcgcg ccggatgatc tcgtgtgcgg ccgaggggcg aatgagttcc
     1201 caccaccggg cgcgccgcga agtcgatacg acgatatggg tgaaattgtt ggcgtgggcg
     1261 taatcgataa tgccggcggc aacattttcg gcgggcacgg agacggccac accgcccagc
     1321 cgctcggcta cccgcaaggc ctcggcgatc cggtcgcgct ctgcctcgct cagacgctgc
     1381 gtgtgtcccg tctcgatatt gatcgcggcc caggaggccc gcaggcgatc cgcgagcctg
     1441 cgcgcgtaac gcaccaacgc aatcgcgcca ggccgctcgc tgaccagtac gagcacgcgt
     1501 tcgctcgcct cccacgggcc ggagatggca tgcgcccgca tgtaattgac catctgctcg
     1561 tcaacgcgct gggcggtgcg gcgcagcgcg agttcgcgca gggcggtcag gtttccgggc
     1621 ttgaagtaat tgtgcaccgc gcgctccgcc tgcgcgggaa cataaacttt cccctcgccc
     1681 aaacgctgga tcaggtcttc gggcgtgagg tcgacgagtt cgacttcgtc ggcgcgatcg
     1741 agaatcgaat ccgacaccgt ctcacgcaca cggatgcggg
//
LOCUS       DASWZT010000043         1928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_72964, whole genome shotgun sequence.
ACCESSION   DASWZT010000043 DASWZT010000000
VERSION     DASWZT010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72964"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VGE92_01075"
     CDS             complement(<1..734)
                     /locus_tag="VGE92_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312432.1"
                     /translation="MAKSKKRKTNAKKPKRAVSVRKPKKTKRSAKKAVRKAKPARAKA
                     KISAAKKSASSKHKTYKILLMGASYGSLLASKLLFGGHSIHLVCLPAEADLINAEGFK
                     VRLPVRGRSEPILLESRKLPGKVTAGPAAGVNPRDYDLVGLCMQEPQYRSPGVRELLD
                     TVGKSRVPCMSIMNMPPLPYIKRIPGLDHKALEAAYTDPRVWDSVDPKKITLNSPDPQ
                     AIRPPDGKANELLVTLPTNFKCARFV"
     gene            complement(763..>1928)
                     /gene="glcF"
                     /locus_tag="VGE92_01080"
     CDS             complement(763..>1928)
                     /gene="glcF"
                     /locus_tag="VGE92_01080"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: protein motif:HMM:NF008434.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEY1312433.1"
                     /translation="HDAPATREVVKHIDRCLSCLACMTTCPSGVNYMHLVDHAREHIE
                     KTYARPLLDRALRGLLARVLPDPGLFRMAVVAGLVARPFAGVFHALGLKRMAAMLRLT
                     PRHKLAPPVERTGKVYPAAGTRRGRVALLSGCINPVLAPTTNEAAIRLLYRHGIEVVV
                     AADEACCGSLVHHMGREEQALAQARKNIDAWTREIEGEGIDAILITVSGCGTTVKDYG
                     FMLRTDQVYVTKAARISSLAKDVTEYLATLDMPYGKAARQLTVAYHSACSLQHGQNVT
                     KAPKELLLKFGFVVKDVPDGHLCCGSAGTYNILQPELAERLRDRKVATIEGLKPDVIA
                     AGNIGCITQIAAGTAIPVVHMVELLDWATGGPVPGGLEEIGARAPESLVEAVL"
BASE COUNT          342 a          607 c          604 g          375 t
ORIGIN      
        1 tccacgaagc gcgcgcattt gaagttcgtc ggcagcgtca ccaagagctc attggctttg
       61 ccgtcgggcg ggcgaatggc ctgcggatcg ggactgttga gcgtgatctt cttgggatcg
      121 accgaatccc aaacgcgcgg gtcggtgtaa gcggcttcga gcgctttgtg atcgagcccg
      181 gggatgcgct tgatgtaggg caacggcggc atgttcatga tcgacatgca cggcacgcgc
      241 gacttgccga ccgtgtcaag caattcgcgc acgccaggcg aacgatattg cggctcctgc
      301 atgcagaggc cgacgaggtc gtaatctctc ggattgacgc cggccgcggg accggccgtc
      361 accttgcccg gcagcttgcg cgattcgagc aggatgggct cgctgcggcc gcgcaccggc
      421 aggcgaacct tgaagccctc ggcgttgatc aaatcagctt ccgccggcag acagacgaga
      481 tggatcgaat ggccgccgaa cagcaatttg gaggcgagca gcgatccgta ggacgcgccc
      541 atcaggagaa tcttgtaggt cttgtgcttt gacgacgccg atttcttggc ggcggatatc
      601 ttcgccttgg cccttgccgg cttggccttg cggaccgcct tcttggccga acgttttgtc
      661 tttttcggct tgcgcacgga gacggcgcgc tttggtttct tcgcattggt tttccgtttc
      721 ttggattttg ccattgtcgt cctcccaaac ttttccgtcg ctttacagga ccgcttcaac
      781 caaactctcc ggagcgcgcg ctccgatctc ctcgagccct cccggcaccg ggccgccggt
      841 cgcccaatct agcaactcga ccatatgcac gaccgggatg gcggtgccgg cggcgatctg
      901 cgtgatgcag ccgatattgc cggccgcgat cacatccggc ttgagccctt cgatcgtggc
      961 gaccttgcgg tcgcgcagcc tctccgccaa ctcaggctgg agaatgttgt aggttccggc
     1021 cgagccacag cacagatggc catcgggcac atctttgacc acgaaaccga actttaaaag
     1081 caattctttc ggcgcctttg tcacgttttg cccgtgctgc agggagcagg ccgaatgata
     1141 ggcgacggta agctgccggg ccgccttgcc gtagggcata tcgagcgtcg ccaggtattc
     1201 ggtcacgtcc ttggcaagcg atgaaatccg cgccgctttg gtgacataga cctgatcggt
     1261 gcgcagcata aacccgtaat ccttgactgt ggttccacag ccggaaaccg tgataaggat
     1321 ggcatcgata ccctcgccct cgatctcgcg cgtccaggca tcgatattct tgcgggcctg
     1381 cgccagcgcc tgctcttcgc ggcccatgtg gtggaccagc gagccgcagc aggcctcatc
     1441 ggccgcgacc accacttcga tgccatgacg atagagcagc cggatcgcgg cctcgttggt
     1501 cgtgggcgcc agcaccggat tgatgcatcc cgacaacagc gccacccgcc cgcggcgagt
     1561 gccggccgcc ggatagacct tgccggtgcg ctcgaccggc ggcgccagct tgtggcgcgg
     1621 cgtgagacgc aacatggccg ccatacgctt caggccgagc gcatgaaaaa cgcccgcgaa
     1681 cggccgtgcg accagtccgg ccacgaccgc catgcggaac agtcccggat ccggcagcac
     1741 acgcgccagc aggccgcgca gcgcgcggtc gagcagcgga cgcgcgtagg ttttctcgat
     1801 gtgctcgcgg gcatgatcaa cgaggtgcat gtaatttaca ccggacggac aggtggtcat
     1861 gcaagccaag caggaaaggc aacggtcaat gtgtttgacc acctcgcgcg tggcgggcgc
     1921 gtcgtgct
//
LOCUS       DASWZT010000044         2745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_58447, whole genome shotgun sequence.
ACCESSION   DASWZT010000044 DASWZT010000000
VERSION     DASWZT010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58447"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /locus_tag="VGE92_01085"
     CDS             <1..1305
                     /locus_tag="VGE92_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY1312434.1"
                     /translation="LVGLGQLMVCLVFGEIVSQFPISGGLYPWARRLVGKRWAWMAGW
                     VYAWALMCTMAGVATGVAPFLAQLFGAQATPVVNTVIALVLIGVSTALNLSGTRWLAR
                     VAMFGFICELVGAIVVGGYLLLFARHQPLKVLLDTTMVHTSGSYWPAFLASSLAAMFC
                     YYGFEACGDVAEETPDASRMIPKAMRLTIYIGGAAAIWICLAFVLSIPNIGAVMSGDD
                     KDPIVTLLRAAMGEAGFRAVIVVVLVSFISCLLSLQAAASRLIFAYSRDQMIFCSKFL
                     SKMSPGHHVPANALIVMGAIPAVIALSALWLQDAIATIISFAAIGIYISFQMIVLAAL
                     IARFKGWRPAGPFTLGIWGWLVNGTALAYGVSAIVNILWPRSPDDPWYSNYAMLVTTI
                     GVLVLGGLYMAIAKPYEHGHAPAGDAHDLRHPTQQPVVEIIG"
     gene            complement(1350..2129)
                     /locus_tag="VGE92_01090"
     CDS             complement(1350..2129)
                     /locus_tag="VGE92_01090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY1312435.1"
                     /translation="MSFAGKIVLVTGSTAGIGEACARLFAESGAGVMLSGRHEARGRG
                     VLAAIRAAGGRAEFTAVDVREPGACERLVGETVSRLGGLDVLVNNAGILYTANALDTS
                     DAQWLDTMAVNVNAVFYLSRAAVKHMKAAGRGAIVNIASEWGLNGEADHVAYCASKGA
                     VIQITRCMALDHARDNVRVNSVCPGEIHTRMVDDILAKRGGNAAQNLKELAAGIPMRR
                     LADPREVAQCVHFLASDLASYVTGTNLSVDGGNDATAGPYP"
     gene            2182..>2745
                     /locus_tag="VGE92_01095"
     CDS             2182..>2745
                     /locus_tag="VGE92_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312436.1"
                     /translation="MPRDPKHDVLFEPISIGPKKLRNRFWQTSHCAGPGAERPGAQAH
                     LRGMKAEGGWGAVFTEFCSIHPESDEYPYISARIWDEGDVVNLGYMCEVAHRHGSLAG
                     IQLWYSGGNAPSLESREFGRAPSQWLSPIFATRSVYGCEMDELDIRTVINMYVAAAER
                     AERAGFDLLEVSAGDDPLPMQFLEPRFN"
BASE COUNT          451 a          899 c          847 g          548 t
ORIGIN      
        1 ctggtgggct tgggtcagct gatggtgtgc ctggtgttcg gcgagatcgt ttcgcagttt
       61 ccgatttccg gcggcttgta tccctgggct cggcgcctcg tgggcaagcg ctgggcatgg
      121 atggccggct gggtctacgc ctgggccctc atgtgcacca tggcaggggt cgccaccggc
      181 gtggctcctt tcctcgcgca gctgttcggc gcgcaagcca cccctgtcgt gaacaccgtc
      241 atcgccctgg tgctgatcgg cgtctctacg gcacttaacc tgagcggcac ccgctggctc
      301 gcgcgcgtcg ccatgttcgg cttcatctgc gaattggtcg gcgccatcgt ggtcggcggc
      361 tatctgttgt tgttcgcccg acatcaaccc ctcaaggtat tgctcgacac gaccatggtc
      421 catacgagtg gcagctactg gcccgccttt ctcgcctcct cgctggccgc catgttctgt
      481 tattacggtt ttgaggcctg cggcgatgtg gccgaggaga cgccggacgc cagccgcatg
      541 attccaaaag ccatgcgtct gacgatttat atcggaggtg ccgctgccat ctggatctgc
      601 ctggccttcg tactatccat cccaaacatc ggtgcggtga tgtcgggcga tgacaaagac
      661 ccgatcgtca cgctgctgcg agcggccatg ggcgaagccg gctttcgggc cgtcatcgtg
      721 gtcgtgctgg tgtcattcat atcctgctta ctgagcctgc aggcggcggc cagccggctg
      781 atcttcgcgt actcgcgcga tcagatgatt ttctgcagca aattcttgag caagatgtct
      841 cccgggcacc acgtccccgc gaatgctctg atcgtgatgg gagcgatccc ggcagtgatt
      901 gcactgtccg cgctatggct gcaggatgcg atcgccacca tcatcagctt tgcggccatc
      961 ggcatttata tttcgtttca aatgatcgtg ctggctgcgt tgatcgcccg cttcaagggt
     1021 tggcggccgg cgggaccctt tacgctcggt atctggggtt ggctggtgaa tggcacggcc
     1081 ctcgcctacg gtgtcagcgc catcgtcaat attctgtggc cacgcagccc cgacgatccc
     1141 tggtacagca attacgccat gctggtgacc accataggcg ttctggtctt gggcggcttg
     1201 tacatggcga tcgccaagcc ctacgagcac ggccacgcgc cggcgggcga tgcccatgat
     1261 ctgcggcacc ccacgcagca gccggtcgtg gaaatcattg gttgactggg ttgcggccac
     1321 gccggcacag cgccgcgcgc cgcgcggtct caaggatacg gacccgcggt ggcatcgttg
     1381 ccgccgtcca cgctcaggtt cgttcccgtc acataacttg caaggtcgga tgccaggaag
     1441 tgcacgcatt gcgcgacttc ccggggatcc gccaagcgcc gcatcgggat tccggccgcc
     1501 agctccttga gattttgcgc ggcattgccg ccgcgcttgg cgaggatgtc atccaccatg
     1561 cgcgtatgga tctcgcccgg acataccgaa ttgacgcgaa cattgtcgcg cgcgtgatca
     1621 agcgccatgc agcgcgtgat ctgaatcacc gcccctttgc tggcgcagta cgccacatga
     1681 tcggcttcgc cattgagccc ccactccgag gcgatattca cgatcgctcc ccggccggcg
     1741 gccttcatgt gcttcacggc ggcgcggctc aggtagaaca cggcattgac gtttaccgcc
     1801 atggtatcga gccactgcgc atcgctggta tcgagcgcat tggccgtgta aagaatgccg
     1861 gcgttgttca ccaggacgtc gaggccgccc aagcggctga cggtctcgcc caccagccgt
     1921 tcgcaggctc ccggctcgcg cacatccacc gcggtgaact ccgcgcggcc gcctgcggcg
     1981 cggatggccg caagcacgcc gcggccgcgc gcttcatgcc ggccggacag cattaccccg
     2041 gcgcccgact ccgcgaacag ccgtgcgcag gcctcgccga tgccggcggt cgagcccgtg
     2101 accagaacga tttttcccgc gaagctcata aaacctcgtt cgcgacggcg tgagcggtgg
     2161 acattataaa caggtgagac catgccccga gatcccaagc acgacgttct gttcgaaccg
     2221 atcagcatcg gcccgaagaa actgcgcaat cgattttggc agacttcgca ctgtgccggc
     2281 cccggcgccg agagacccgg cgcacaagcg cacctgcgcg gcatgaaagc cgagggaggt
     2341 tggggcgcgg tcttcaccga attctgctcc atccatcccg aatcggacga gtatccctat
     2401 atctcggcgc gcatctggga cgagggcgat gtcgtcaact tgggctacat gtgcgaagtg
     2461 gcgcaccgcc atggaagcct cgccggcatt cagctgtggt acagcggcgg caatgcgccg
     2521 tcgctcgaat cgcgcgagtt cggccgcgcg ccgagccagt ggctctcgcc catattcgcg
     2581 acgcgctcgg tgtacggctg cgaaatggac gaactcgaca tccgcaccgt catcaacatg
     2641 tacgtggcgg cggccgagcg cgccgaacgg gcgggattcg atctgctcga ggtctcggcg
     2701 ggcgacgacc ccctgccgat gcaatttctc gaaccccgct tcaac
//
LOCUS       DASWZT010000045         4009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_43969, whole genome shotgun sequence.
ACCESSION   DASWZT010000045 DASWZT010000000
VERSION     DASWZT010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43969"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VGE92_01100"
     CDS             complement(<1..528)
                     /locus_tag="VGE92_01100"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY1312437.1"
                     /translation="MVQTARPLTRILGLGFGLAMVFGGTVATGILRLPGTLAAALGDS
                     RLIVLFWIVGGIYSLLGAVAVAELAAMLPQAGGFYVYAKRAFGNGAGFAVGWSDWVNS
                     VATIAYAALTSAEFIGVLWPTAAAAPRAIAIGVLCVFTILHWTGLRIGSGLTRLISLA
                     VGLMMMVLVVGCFVGA"
     gene            complement(577..768)
                     /locus_tag="VGE92_01105"
     CDS             complement(577..768)
                     /locus_tag="VGE92_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312438.1"
                     /translation="MSELSKLKKENKQLKLLLKDAVKMLDRYKDILKHPERLLPPVKK
                     ARAKTAKARAAQTRTASDT"
     gene            complement(863..2107)
                     /locus_tag="VGE92_01110"
     CDS             complement(863..2107)
                     /locus_tag="VGE92_01110"
                     /inference="COORDINATES: protein motif:HMM:NF017380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HEY1312439.1"
                     /translation="MHRRTGSANLPLHGGHVPAWLSTRMAALGRVIVEAIVLEYGRDE
                     VLRRLAHPFWFQSFGAVMGMDWHSSGITTSVIAALKRGLAPLQRELGLYVCGGKGAHS
                     RRTPQELIRIGEKTGVDASHWVQASRLVAKIDSAAVQDGYDLYLHAFIATDDGHWCVV
                     QQGMNETLREARRYHWQSENLSGFFDSPHSAVEGRNLGCIVNLADARAGRNRRAALGL
                     VHEGPDRTLAVLRRYSQNLALDLFSETLPAEPPLPHLQLPHRHEVAASDVLLRRLHGT
                     LTAAANNGPRDFADLLLTPGVGARTVASLAFVAEVLYGAPSRFSDPARFALALGGKDG
                     HPFPVPLKVYDATIQVLKRAVMQAHLGSSETLAAVRRLDAQARLLEASSTGVSFESFV
                     AGERDQSAALGGRSVTAHAHRP"
     gene            2323..2661
                     /locus_tag="VGE92_01115"
     CDS             2323..2661
                     /locus_tag="VGE92_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312440.1"
                     /translation="MRTIFMIALMSAALFGALMFAGSLILLCIRQLRAWGTAALTGGV
                     SGAVAAFALFAALVLFGELGGTSLFSELSASIAAAGFGTGAFFGAAGLLLSLLMQSPS
                     RWADRRRLKH"
     gene            complement(3104..3760)
                     /locus_tag="VGE92_01120"
     CDS             complement(3104..3760)
                     /locus_tag="VGE92_01120"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY1312441.1"
                     /translation="MRNDPNAALLTAAMLAACLAATVTFGDSVFHDKPASPRSLPADN
                     LPPFGMLDEQGRLVPVPPMPAGGLHRGPGSPPESTAAGPNLADSIAAARAAVAACSAA
                     GYRIGATVIDSSGEARAMLTADGSDGSHVFVAMRKAITALTFKMPSAQVRALVEKDKA
                     QLARVTPAMFVEGGAVPITADGKVIGAIGASGAGGSPIGHQDESCAIAGLNAIRAQSK
                     "
     gene            complement(3772..>4009)
                     /locus_tag="VGE92_01125"
     CDS             complement(3772..>4009)
                     /locus_tag="VGE92_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312442.1"
                     /translation="APLYYPTPIRGTGVGAAVALGRLGSVVGPLFAGGLLAGGGGSVA
                     VLLAIVPFVAVGGGAAFALTWRKQCEFDQSVPTN"
BASE COUNT          761 a         1302 c         1233 g          713 t
ORIGIN      
        1 tgcgcccacg aaacaaccga ccaccagcac catcatcatg aggcccaccg caaggctgat
       61 gagccgggtc aagccgctgc cgatgcgcag gcccgtccaa tgcaaaatcg tgaagacgca
      121 cagaacgccg atggcgatcg cgcgcggcgc tgcggcggcg gtgggccaca gcacgccgat
      181 gaattcggcg gaggtgaggg ccgcgtacgc aatggtcgcg actgaattga cccagtcgct
      241 ccagcccacc gcgaaacccg cgccgttgcc gaaggcacgc ttggcataga cataaaagcc
      301 gcccgcctgc ggcaacatgg ccgcaagctc cgccaccgcc accgcgccca gcagcgagta
      361 aatgccaccg acaatccaga acaagacgat gaggcgcgaa tcgcccaagg ccgcggccaa
      421 cgtgcccggc agccgcaaaa tacccgtggc gacggtgccg ccgaaaacca tcgccaagcc
      481 gaaaccgagc cccagaatgc gagtcaaggg tcgagctgtt tggaccatga cgcagcttgc
      541 gcgagagcgg cgaaagccgc aaggcggctg tcggcgctat gtatcgctcg ctgtccgagt
      601 ttgcgccgcc cgcgccttgg ctgttttcgc gcgtgctttt ttcaccggcg gcagcagcct
      661 ttcgggatgc ttcaaaatgt ctttgtaccg gtccaacatc ttgaccgcat ccttcaacag
      721 taatttcagc tgcttatttt ccttcttcag cttgctcaac tcgctcatgc ttccctccat
      781 ctgtagccgt cggcgttccg agagagcgcc gtgttcgagt ttagaccaaa cgccgcgaag
      841 cgctgtcgcg ctcgatgttt ccctatggcc gatgagcgtg cgctgtcacc gatcgacccc
      901 cgagggcggc cgactgatcc cgctcgcccg cgacaaaaga ctcgaacgac acacccgtcg
      961 agctcgcttc cagcagccgc gcctgggcat cgagacgccg aacagcggcc aaggtttcgc
     1021 tcgatcccaa atgggcctgc atcacggcgc gcttcagcac ctggatggtg gcgtcgtaga
     1081 ccttgagcgg aaccggaaac ggatgtccgt cctttccacc gagcgccagc gcaaagcgcg
     1141 ccgggtccga aaagcgcgag ggcgcgccgt acaggacttc cgcaacgaac gcgagcgagg
     1201 ccacggtgcg tgcgccgaca cccggggtca acaacagatc agcgaaatcg cgaggtccgt
     1261 tattcgcggc ggcagtcaag gtgccatgca agcgtctcaa caagacgtcc gaggccgcga
     1321 cctcatgacg atggggcaat tgcagatggg gcagcggcgg ctcggcggga agggtctccg
     1381 aaaatagatc gagcgcgagg ttttgagaat aacggcgcaa aaccgccaac gtacggtcgg
     1441 gcccctcatg cacgagtccg agtgccgcgc gtcgatttcg cccggctctg gcatcggcga
     1501 gattgacgat gcatccgagg tttcggccct ccaccgcact gtgcggcgaa tcaaagaaac
     1561 cggacagatt ctccgattgc cagtgatagc gcctggcttc gcgcaacgtt tcgttcatgc
     1621 cctgctgcac gacgcaccag tggccgtcgt cggtggcgat gaatgcatgt aagtacaggt
     1681 cgtatccatc ctgcacggcg gcagaatcga tttttgcgac tagcctgctc gcttggaccc
     1741 agtgagatgc gtcaacgccc gtcttctcac cgatccgaat cagctcctgc ggggtacgac
     1801 gggagtgcgc gccctttcct ccgcacacat acaggcccaa ttcacgttgc agcggcgcaa
     1861 gaccgcgctt cagggccgcg atcacgctcg tcgtgattcc cgaggagtgc cagtccatcc
     1921 ccatgacggc tccaaaggat tggaaccaga acgggtgtgc cagtcggcgc agcacctcgt
     1981 cgcggccgta ttcgagcacg atcgcctcga cgattacacg cccgagtgcc gccatgcggg
     2041 tcgacagcca cgccggaacg tggccgccat gcaggggtaa gttagcgctg ccggtacgcc
     2101 ggtgcatgca gccatggtac acgccgtgct cgcattggta ttgaaatcaa tgcctctgca
     2161 cggataggtc gcgtgacact cgaagaagat cacgccgtga tgggcagata ccttcgctgc
     2221 tgcaggtaaa atctgagcgg atcgatcatt aaataatcat gcgaccgtaa cgctttccct
     2281 ggtaaaaggc ctacattgac atcgacaccc gaggaggccg ctatgcgtac gattttcatg
     2341 attgctttga tgtccgcggc gttgttcggt gcgttgatgt tcgccggcag tctgattctg
     2401 ctgtgcattc gccaattgcg cgcctggggg acggcagccc tgaccggggg tgttagcggc
     2461 gccgttgccg cgtttgcatt gtttgcggcg ctggtactgt tcggagaact cgggggcaca
     2521 tccctcttca gtgagctctc cgcgtcgatc gccgcggccg gcttcgggac cggcgccttt
     2581 ttcggcgccg cgggactgtt gctcagcctg ttgatgcagt ctcccagtcg ctgggcggat
     2641 cgccgacgct tgaaacactg acccgctaca cgccgatctc ctttaaggat cactcgaggc
     2701 agccgtcagg ctacgcgatg agcctattcg ggcggattcc tctcgtgctc gcgggggaat
     2761 tcttccgtgg tcccctcatt gctcggcaac ggcggctcgc gaacatcggg cggcgagtcc
     2821 tcgcccggat cgcgcctcgg attagggatc gtttcgatgc tggcggccgg actcattcgc
     2881 ttcctttggc cggattgcgc ggtgctccga tgcaccatgc tgcaaaaacc acgatcaaaa
     2941 tcgcgccgat gtagacggga agggctggca tgcgaacctc cgatgcgaaa gattgtgaga
     3001 gtgcgaccgc gtgccaggat gaaacggcta cgcaggcgcg tctgtaggcg cagttccata
     3061 ccggtcgctg aattttctca cagcctgaag atcggctgcg atgctacttc gattgcgcgc
     3121 gaatcgcgtt gagcccggcg atcgcgcaag actcatcctg atgtccaatg ggcgagccac
     3181 ccgcgccgct cgcaccgatc gcgccgatca ctttcccatc cgccgtgatg ggaacggctc
     3241 cgccttcgac aaacatggcc ggcgttacgc gcgccagctg cgccttgtct ttctcgacca
     3301 gcgcccgcac ctgagcactc ggcatcttga aggtgagcgc ggtgatggcc ttgcgcatgg
     3361 cgacgaacac gtggcttccg tccgagccat ccgcggtcaa catcgcgcgc gcctcgccgc
     3421 tcgaatcgat cacggtcgcg ccgattcgat agcctgccgc ggaacatgcc gcgaccgccg
     3481 cgcgtgccgc cgcaatcgag tccgccagat tcggaccggc ggccgtggac tcgggcggtg
     3541 agcccggccc gcgatgcagg cctccggccg gcatcggcgg gaccgggacc aaacggcctt
     3601 gctcgtccaa cataccgaac ggcggcagat tgtcggccgg caggctgcgc ggcgatgcgg
     3661 gtttgtcgtg aaagacgctg tcgccgaagg tgacagtcgc tgcgagacag gccgccagca
     3721 tcgctgcggt cagaagtgcc gcattcggat cattgcgcat ggagtttctc cttagtttgt
     3781 tggaactgat tgatcgaact cgcactgctt acgccacgtc agtgcgaacg cggcgccgcc
     3841 gccgacggcc acaaacggca cgatggcgag cagtaccgcg acgctgccgc cgccgcctgc
     3901 cagcaagccg ccggcgaaca aggggccgac gacggaaccg agcctgccga gcgcgaccgc
     3961 ggcacccacc ccagtgccgc gaatcggcgt ggggtaatac aacggcgcg
//
LOCUS       DASWZT010000046         1731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_77816, whole genome shotgun sequence.
ACCESSION   DASWZT010000046 DASWZT010000000
VERSION     DASWZT010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77816"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VGE92_01130"
     CDS             <1..823
                     /locus_tag="VGE92_01130"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEY1312443.1"
                     /translation="ADVVVLCVGEAATMSGEAASRAHLGLPGQQRQFAEAVFERARAA
                     AKPVIVVLFSGRPLVVPWLVEKADAVLAAWFLGSEAGNSIADVVTGRVSPSGRTPVSW
                     ARAMGQIPIFYGERPSGRPADPKDHYTSKYLDMPNEPLFAFGHGLTYGQFILTNLRVT
                     PRNVEDIDTMQIRVDVRNVGAREAQETVFLFVHHKVASVARPLLELKGFAKVNLRPGA
                     TGSVAMSLCATELRYLGVDLQPVFEPGEVEILVGPCADRSQLLVATIDLSWSRPC"
     gene            complement(803..1369)
                     /locus_tag="VGE92_01135"
     CDS             complement(803..1369)
                     /locus_tag="VGE92_01135"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HEY1312444.1"
                     /translation="MRRTISGCLAAAVLATAIAQTPEETKVWEAQRAERQAAEKSKAE
                     QLARERAARRADPMAWVRTLDPMSAGGWVFRAVAADGSWATFSTEHQLKRSGHLVTAW
                     LRQEFPEPQHSPGGEAYFSDVEKVQYDCAKERARVMLIIYYTGNNLSGGQQSEEADPK
                     QVEWDPIVPGTQSESIFHWTCGSTGTRP"
     gene            complement(1366..>1731)
                     /locus_tag="VGE92_01140"
     CDS             complement(1366..>1731)
                     /locus_tag="VGE92_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312445.1"
                     /translation="IHQQSGNEPLIVGMDRYAIASELAFYGAERTASAPETSSAHLFG
                     GIGLMYERWTPAVSQQGRTLLLIAWSPGEIAGRSIESHAGHLGPIEEGVLRRDGKVVR
                     HYYQRLAYNYRIIPPEVAQ"
BASE COUNT          282 a          549 c          552 g          348 t
ORIGIN      
        1 agccgatgtc gtcgtgttgt gcgtcggcga agcggcgacc atgagcggcg aggccgcgag
       61 ccgcgcccat ctcggccttc ccggtcaaca gcggcagttc gcggaggccg ttttcgagcg
      121 cgcgcgcgcc gcggccaagc ccgtcatcgt ggtattgttt tcagggcggc cgctggtggt
      181 tccatggctg gtcgagaagg cggatgccgt gctcgcagcg tggttcttgg gcagcgaggc
      241 cggcaattcg atcgccgacg tggtcaccgg ccgggtgtcg ccgagcggcc gaacgccggt
      301 cagttgggcg cgggcgatgg gtcagatacc gatcttctac ggcgagcggc cgagcggccg
      361 gcccgcggat ccgaaggatc attacaccag caagtatctt gacatgccga acgagccgct
      421 gtttgccttc ggccacggcc tcacgtacgg gcagttcatc ctcaccaatc tgcgggtcac
      481 gccgcgcaat gtggaggata tcgataccat gcaaatccgc gtcgatgtgc gcaatgtggg
      541 tgcgcgcgaa gcgcaggaga cggtgttcct attcgtgcat cacaaggtcg cgagcgtggc
      601 gcggccgctg ctcgagctca aaggtttcgc caaggtcaac ttgcggcccg gcgcaaccgg
      661 cagcgtcgcg atgtcgctgt gtgcgacgga actgcgctat ctcggcgtgg atcttcagcc
      721 ggtgttcgag ccaggcgaag tcgaaattct ggtgggtccg tgcgccgacc gatcgcagct
      781 gctggttgca acgatcgatc tgtcatggtc gcgtccctgt tgagccgcaa gtccaatgaa
      841 aaatggattc gctctgggtg cccggcacga tcgggtccca ctcgacctgc ttcgggtccg
      901 cctcttcgct ctgttggccc ccggagagat tgtttccggt gtagtagatg atcaacatga
      961 ctcgcgcacg ctccttggca cagtcgtact gaaccttctc gacgtcgctg aaatacgcct
     1021 cgcctcccgg actgtgttgc ggctcgggaa actcttgcct gagccatgcc gttaccagat
     1081 ggccggaacg cttcagttga tgctcggtgc tgaaggttgc ccatgatcca tccgctgcaa
     1141 ccgccctgaa cacccaaccg cccgcagaca tggggtcgag ggtgcgcacc cacgccatgg
     1201 ggtccgcacg ccgagcggca cgttcgcgcg cgagttgctc cgctttggac ttctccgccg
     1261 cttgcctctc ggcgcgctgc gcctcccaca ctttcgtctc ctctggggtt tgggcgatgg
     1321 cggtggcaag cactgcggct gcaagacacc cagaaatcgt ccgtctcatt gcgctacctc
     1381 cggcggaata attcggtaat tgtaggcgag cctctggtag tagtggcgta caaccttgcc
     1441 gtcccgcctc aacaccccct cttcgatcgg acccaggtgg ccggcatgag attcaatcga
     1501 tcggccggca atttcccccg ggctccaggc gatgagcagt aaggtgcgtc cctgctgcga
     1561 tacggccggc gtccagcgct catacatcag cccgattccg ccgaacaaat gggcactcga
     1621 ggtttcgggc gccgatgccg tgcgttcggc cccgtaaaat gccaactcgc tggcaatggc
     1681 gtagcggtcc atgccgacga tcagcggctc gttgccgctc tgttggtgga t
//
LOCUS       DASWZT010000047        20831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_34294, whole genome shotgun sequence.
ACCESSION   DASWZT010000047 DASWZT010000000
VERSION     DASWZT010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 20831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20831
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34294"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..537)
                     /locus_tag="VGE92_01145"
     CDS             complement(1..537)
                     /locus_tag="VGE92_01145"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1312446.1"
                     /translation="MTTPGPVSFKSRRLAIHPFDEACVSDEYLSWLNDQEVMRFSNQR
                     FRAHSRQSARDYIASFAGTTNVFLAVRLAESGRMIGTMTAYISNQHTTADMGILIGDR
                     ACWGQGYGLEAWTTLMHNLFASWGMRKVTGGAARPNMGMVRIMERSGMHLEAVRTRQE
                     IIDGVEQDVLYYARFVDR"
     gene            complement(534..1811)
                     /locus_tag="VGE92_01150"
     CDS             complement(534..1811)
                     /locus_tag="VGE92_01150"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY1312447.1"
                     /translation="MKEKLALHGGAPVIAKAFPPYRSLGAEEIEAANRVLKTGVLSAY
                     IGAPGEGFMGGPEVRALEAEAAAYFGVRHAVAVNSWTSGLISAVGAIGTEPGDEIIVT
                     PWTMAASATAIVHWNAIPVFADIDEESFNLDPNAVEAAITPRTRAIMSVDIFGQSADM
                     ERLRSIAARHKLTLLSDSAQAPGARYKDKYAGTLADIGGYSLNYHKHIHCGEGGILVT
                     DDDRYAQRLRLIRNHAEAVIKSEDPAELSNMIGYNFRLGEIESAITREQLRKLAAKVA
                     ARQDAARRVAEGLSGLPGLILPKVSAQCTHVYYVFGMRLQSERTNVPRAAIIAALRAE
                     GVTGLGSGYQNVHRLPMFERGIAYGKNGFPWLGLGADCSRRASTQRCPVAERLHEQDF
                     FFLNICAHEYTPAEIELVVAAFYKVWANLQNLG"
     gene            complement(1808..2806)
                     /locus_tag="VGE92_01155"
     CDS             complement(1808..2806)
                     /locus_tag="VGE92_01155"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY1312448.1"
                     /translation="MWRAVVVGCGWIGSGVADDPRADGIQSHAAAYAACPDTELAGVC
                     DADAACAQAAATRFGLGRGFHNLSDLLSSTRPQIVSVCTPDATHAAVLREVLNHAGVR
                     AVIAEKPLATDLAEARALVELADSRGVILAVNYSRRFAPSHIEARRRLIAGDLGMVQA
                     VQGCYTKGIMHNGTHWFDLAQWMVGKIARVQAWSGHAGGRQPDPTCHVRMMFADGQTG
                     FLLGLDEERYTIFEMDILGSLGRLRLMDSGVRISWDSVADSPHFSGYRTLQPSGEIAS
                     GFKDVALRLVEDVVAALGQGRQAKCSGRDGVAALAAADAARRSLATGIECNVQCGS"
     gene            complement(2806..3852)
                     /gene="pseI"
                     /locus_tag="VGE92_01160"
     CDS             complement(2806..3852)
                     /gene="pseI"
                     /locus_tag="VGE92_01160"
                     /EC_number="2.5.1.97"
                     /inference="COORDINATES: protein motif:HMM:TIGR03586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid synthase"
                     /protein_id="HEY1312449.1"
                     /translation="MIRIGKHEVGEGAPPFIIAEMSGNHNGSLSRALEIVDAIAASGA
                     HALKLQTYTADTMTIDVAEREFVIRNPESPWFGRNLHALYEEAHTPWKWHKPLFQRAR
                     EHGLAAFSSAFDGTAVDFLESLDVPAFKIASFENRDLDLIRRVARTGKPVIISTGLAT
                     LAEIDAAVRAVHEAGGHDLVLLKCTSNYPADPSSSNLRTIPYLRDLYRCEVGLSDHTL
                     GIAVATASVACGATVIEKHVTLDRASGGVDSTFSLEPPELKQLVESCRMAWLALGSVQ
                     TLPTAEEIPSLVFRRSLYVCTDLAAGDALTRENLRAIRPGNGLPPQHLSMLLGKRVNR
                     TVPRGTPMSWDLIG"
     gene            complement(3849..5372)
                     /gene="pseG"
                     /locus_tag="VGE92_01165"
     CDS             complement(3849..5372)
                     /gene="pseG"
                     /locus_tag="VGE92_01165"
                     /EC_number="3.6.1.57"
                     /inference="COORDINATES: protein motif:HMM:TIGR03590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,4-diacetamido-2,4,
                     6-trideoxy-beta-L-altropyranose hydrolase"
                     /protein_id="HEY1312450.1"
                     /translation="MATEAPRSIAIRVDGGTRMGSGHVIRCLSLAGRLAGNGAAITFI
                     CRPLPGDLVENIEREGYPVVRLTPSAGAADSKAAVNWLQVPKLQDRTETCEVLEQLRS
                     ECLIIDHYGIDAEWERAQRRWVKRIVVIDDLADRPHDCDVLLDQNFFGCQTGNRYAGL
                     VPTHCRLLLGPRYALLQRDYANLRAALVPRRGAPRRVLVFFGASDATDETSKALRALQ
                     APELAHLAVDVVLGVNHPAPEAVAAAAAQRPGTAIFKDLSTLAGLMFRADLAIGAGGV
                     ATSERLCMGLPSIVVTLAPNQEGPVSHLADAGAVIWAGRAASVSVAELTTAVSCALRS
                     AADPPPLVDGHGVARAGAAIVPPLPSRLRLRRARIADAGLLFDWRNEPLTREMSFDSS
                     PIVWQTHFEWFQAKLSDRNSDMVIGEVEGLPAGQARLDFRNDEAVLSFSVDPDLRGLG
                     FGRALVERALLFGRSRAARRFIARVKPENAASRNIFQQLGWRETMVDGGLEYRGGQR"
     gene            complement(5362..6087)
                     /gene="pseF"
                     /locus_tag="VGE92_01170"
     CDS             complement(5362..6087)
                     /gene="pseF"
                     /locus_tag="VGE92_01170"
                     /EC_number="2.7.7.81"
                     /inference="COORDINATES: protein motif:HMM:TIGR03584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid cytidylyltransferase"
                     /protein_id="HEY1312451.1"
                     /translation="MSEQPPPGSALAIIPARGGSKRIPRKNIRPFVGVPMLARTIAML
                     RAANAFDRIIVSTDDDEIADVALQAGAEVPFRRPAELSNDMAATLPVIVHAIREIESR
                     GCSARFVCCAYPAAVLSRPSDIRSARDLLDAEDVDYVFTATLFPYPIQRALRKISGGY
                     CEMYWPENRETRSQDLEPAYHDAGQFYFGRRDSWLEARPIFGPRSKMLELPHNRVQDI
                     DSPEDWIRAEMIFRLLEQEERGD"
     gene            complement(6080..7231)
                     /gene="pseC"
                     /locus_tag="VGE92_01175"
     CDS             complement(6080..7231)
                     /gene="pseC"
                     /locus_tag="VGE92_01175"
                     /EC_number="2.6.1.92"
                     /inference="COORDINATES: protein motif:HMM:TIGR03588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine transaminase"
                     /protein_id="HEY1312452.1"
                     /translation="MTSRFIPYGRQVVEDEDIAAVAEVLRSDWLTTGPVVERFELAFA
                     AYVDARYAITASSGTAALHLCMLAAGIGSDDEVIVPALTFVASANCARYQGTRIVFAD
                     VRADTLTVDAAHVESLITPRTRAIVAVDYGGLPADLDELSELAHRHRLLLIEDACHAP
                     GAEYRGRKVGSIADMTAFSFHPVKHLTTAEGGMVATNDADLALRLRRFRNHGIDSDHR
                     KREQQGTWRYDMVDLGFNYRLNDVQCALGLSQLRRLPQSLERRHALAARYAEAWQSSP
                     LLRIQFEPQDRKHSWHLYAVRLAGENPGEPRERVFNALRALHIGVNVHYLPVYLHSYY
                     QELGYARGLCPVAEHAYAGLLSLPMWHGLTDADQDRVIESVNLVVERHV"
     gene            complement(7224..8222)
                     /gene="pseB"
                     /locus_tag="VGE92_01180"
     CDS             complement(7224..8222)
                     /gene="pseB"
                     /locus_tag="VGE92_01180"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HEY1312453.1"
                     /translation="MFSNKSILVTGGTGSFGRAFVKSVLRSHPEFNRLVVYSRDELKQ
                     FEMAQVFSDRQHKGMRYFLGDIRDEGRLRRALEGIDIVVHAAALKQVPAAEYNPFECI
                     KTNVLGAQNLIDACLDSGVSRVVALSTDKAAAPINLYGATKLCSDKLFIAANNIKGSR
                     DIRFSVVRYGNVMGSRGSVIPFFLDKRSSGVLPITDPNMTRFNISVQEGVDMVLWAIE
                     HAWGGEILVPKIPSYRVTDVAVAVDSKCEQRVIGVRPGEKIHEEMITASDSFNTVDLG
                     RYYAILPSGGQYSLETYCEERGAKPVEPGFSYNSGSNPNFLTVDELRLLIAQEIND"
     gene            complement(8230..9120)
                     /locus_tag="VGE92_01185"
     CDS             complement(8230..9120)
                     /locus_tag="VGE92_01185"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEY1312454.1"
                     /translation="MKTPAAVFEDEDTIDSRRIIAALRAKRWWLLGSVLFFTAAFGAA
                     AFVMTPIYRSTTILVASNANQGSGGLGSALGDLGGIASLAGLNVGSRTSVTEEALAVL
                     RSREFTERFIVDERIMTRLFFKKWDAASNQWKGEPPTLDKAYRYFNQKIRSVDQDKKT
                     GLISVQIDWRDRNEAAKWTNDLVAQLNQEMRSRAISDADASLGYLDKELQTTTVVTAH
                     DAIGHLIESQVKQRMLANVSKEFVFRVVDRAMPADVDDPIKPQKGTMIAAGFTLGLMV
                     GVIFALVTDGSRPRTAVVTH"
     gene            complement(9130..12141)
                     /locus_tag="VGE92_01190"
     CDS             complement(9130..12141)
                     /locus_tag="VGE92_01190"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEY1312455.1"
                     /translation="MRTRSLGALLAALSIGLVGSTQSQNSDTLQALKNSLSPDQQGSI
                     LQDVLGGKGAGSGKKVDPKLDTPDTVRRANGDQAEKERAQRTLDGRTLRQSDEDPELR
                     GEDTVVIELISNDDLCNAKPNQNAEQNNNNNPQSNANQAIGAIGANGIPVFNGVNGAA
                     GALGATTGTSVRGATGNAPNNNFSNTFCRPMPVPKTDLEKERTEKFRKRILDNNPYRL
                     NGFGVLEMPGLPAIPLAGLTASEATKRLGADPDLADFAVRLTLLRLMPSGNDALKPFG
                     YDLFQGVPSTFAPVSDIQVPADYIVGPGDTFNIQLYGNESSRYELTVERDGKLNFPKL
                     GPIVVSGMSFDAARETIEQRVAKQLIGTRVSVTMGDLRSIRVFVLGEAEKPGSYTVSG
                     LSTMTNALFVSGGVKKIGSLRTIELKRNGRLVTTLDLYDLLLHGDTSGDRQLLPGDVI
                     FIPPIGRTMAVDGAVRRPAIYELKGEKTVGQAIEIAGGLLSDADEKLGQLERILPSRL
                     HEMRNIDLTKPAGRGMEIADGDKLRIPAIRPTLENSVVLTGFVFRPGQFEYHPGLRLT
                     DILTSFDELRPNADQRYIMIRREVPPNETVQVLSADLQRALAARGSAADPELRPRDKI
                     YVFDLSTDRERILGPVIRQLQLQATPEQPEQVVSIDGRVKAPGHYPLEPAMHVSDLIR
                     AGGSLEDAAFRGEAEITRYEVTPGDARKTELIAVDLAAIRRGDAGADLLLKPYDVLII
                     KPIPQWVEPGVIELAGEVRFPGKYPIHQGETLSSVLQRAGGFTDLAFIEGAVFIREEL
                     KKREKDQLELLANRLQSDLAALSLEAITGSAATNSGSGNGAAQGLAVGQQLMNQLRDT
                     KPVGRLVIDINRVVKRPAGGSEDVALRDGDKLLIPKKTQEITILGEVQSPTSHVFEAG
                     LTRDDYIARSGGTTQKADRKRIYVVRANGDVSSGERSGWFRRSQSVEMRPGDTIVVPL
                     DTERIRALPLWQAVTTIIYNLAVALLAVRSV"
     gene            complement(12250..13362)
                     /gene="rfbB"
                     /locus_tag="VGE92_01195"
     CDS             complement(12250..13362)
                     /gene="rfbB"
                     /locus_tag="VGE92_01195"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: protein motif:HMM:TIGR01181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEY1312456.1"
                     /translation="MSYEPHNLFITGGAGFIGANFARHWLSSTSEGRVVVFDALTYAG
                     NIENLAGLDQNPRYLFVKGDICDEATVRALLEQHQIDTIAHFAAESHVDRSILGPDDF
                     IRTNIVGTHSLLKAAKTLWIDRKTVAAHRFHHVSTDEVYGSLGPQDAPFRETTPYAPN
                     SPYSASKASSDHLVRAYHETYGLVTTVTNCSNNYGPYQFPEKLIPLTIVNILLGKPLP
                     VYGDGLQVRDWLHVEDHCVAIALALRRAKSGEVYNVGGDSETTNIEIVRTLCALTDKA
                     LGERADLRAAFPASPAVQGKRAVDLITHVRDRPGHDRRYAIDYAKAARELGYAPAGNL
                     VNGLGATLDWYLANIGWWKALLGRDYADWLNKNYKQ"
     gene            complement(13343..14206)
                     /gene="rfbD"
                     /locus_tag="VGE92_01200"
     CDS             complement(13343..14206)
                     /gene="rfbD"
                     /locus_tag="VGE92_01200"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEY1312457.1"
                     /translation="MKVLVLGGGGQVANAVVAAAGEGREVLVRSRAQLDIGDEKAVAD
                     ALAETRAQWIINAAAYTAVDLAEDHPAQAIAVNDTAVGVLASAATASACRLLHLSTDF
                     VFDGQSNRAYLPGDQPNPLSVYGTSKLGGERQALRGATGIVLRTAWVYAAAGKNFVLT
                     MLRLMQEKEQVSVVSDQIGTPTWAGSIAAAIWGLIEIGAPGGIYHWTDLGVASWYDFA
                     VAIQDEALARGLLSRAVPVNPIRSAAYPTRARRPAFSVLDTVSTRTLIKVPARYWRHN
                     LRTMLDELRTT"
     gene            complement(14203..14751)
                     /gene="rfbC"
                     /locus_tag="VGE92_01205"
     CDS             complement(14203..14751)
                     /gene="rfbC"
                     /locus_tag="VGE92_01205"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEY1312458.1"
                     /translation="MKFTKTPIADVTLIEPQVFGDARGFFMESWHEAKFKAAGLDATY
                     LQDNHSRSSQWTLRGLHLQVQHTQGKLVRVALGSVFDVVVDLRRSSPTFGKSWGVELS
                     ADNHRILWVPPGLAHGILITSESADFLYKCTDFYSPEHERTLAWNDPTLEIRWPLPAG
                     VTPKLSAKDARGASFAEIEKFA"
     gene            complement(14765..15652)
                     /gene="rfbA"
                     /locus_tag="VGE92_01210"
     CDS             complement(14765..15652)
                     /gene="rfbA"
                     /locus_tag="VGE92_01210"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEY1312459.1"
                     /translation="MKPRKGIILAGGAGTRLHPITMSLSKQLLPVYDKPMIYYPLAAL
                     MSAGIREYLIITTPRDQGAFRDLLGTGAQWGLSFEYVVQAVPNGIAQALIIGESFLDG
                     APSALILGDNIFYGANLTALLMSAAQRESGATVFGYYVQDPERYGVVQFDAAGRVTDL
                     LEKPKLPPSNYAVTGIYFYDGRAPAFAKALKPSPRGELEITDLNRAYLQVGDLHVERL
                     GRGTAWLDTGTHNSLLDAGVFVRIIEERQGLKVACVEEIAWRSGFISAEDLVKLAKPL
                     AKSGYGEYLLRIVNEHSQL"
     gene            complement(15740..17362)
                     /gene="pgi"
                     /locus_tag="VGE92_01215"
     CDS             complement(15740..17362)
                     /gene="pgi"
                     /locus_tag="VGE92_01215"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEY1312460.1"
                     /translation="MTQINSSLAEWHALAAHAGDIAGAHLRDLTAADPQRWQNFHVEH
                     DAWLLDMSRQRITGKTLSLLIDLARAAQLPARMAAMFRGDLINSTEQRAVLHTALRSD
                     FAGSAQIQSEVRESRKQLVAFVAAVRSGRKLGITGKKFKHVVNIGIGGSDLGPLLVCE
                     ALRHEWSGDITPHFVSNVDRTQLEDLIRSIDPAETLVIVCSKTFTTLETQANANAARA
                     WVVGALGEKSPANHFVAVSTNAPAMDAFGIAADHRFTMWDWVGGRYSVWSAIGLIAEL
                     SVGSGRFEEFLSGASDIDRHFTQAPFEKNLPVLMGLLGVWNRTFLELPTLAVLPYDQR
                     LARLPAYLQQLEMESNGKSVSLAGGRVDFATAPIVWGEPGSNAQHSFFQMLHQGTLTQ
                     ALDFIAPLRGSWGGTEAQDLALQNCFAQAQAFAFGHSLEEVQADMRRAGAGPEEIARI
                     GAHRVYDGNRPSSLLLYPQTTARSLGGLLALYEHSVFVQGTVWGINSFDQFGVELGKK
                     LAVGIDMKGGRAPAGHGAAGLGSLIDYVARHR"
     gene            complement(17359..18087)
                     /gene="dnaQ"
                     /locus_tag="VGE92_01220"
     CDS             complement(17359..18087)
                     /gene="dnaQ"
                     /locus_tag="VGE92_01220"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEY1312461.1"
                     /translation="MRQVVLDTETTGLEVEQKHRVIEIGCVELVHRRLTGRTYHQYLN
                     PQRDIDEGAQAVHGLTRERLAREPSFAEVHPQFLEFIRDAELIIHNAPFDIAFLNAEL
                     ALIDVAHRVDDLCRVLDTLAMARQMHPGQRNNLDALCKRYSVDNSHRDYHGALLDARI
                     LAEVYLAMTGGQAMLSLSAESETARSRARQAPARLNAAVRIIVVRPSEQEMDAHEHVL
                     ALLDKASGGRTVWRRNESSETGVT"
     gene            complement(18097..18540)
                     /gene="rnhA"
                     /locus_tag="VGE92_01225"
     CDS             complement(18097..18540)
                     /gene="rnhA"
                     /locus_tag="VGE92_01225"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003180633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEY1312462.1"
                     /translation="MTQVDIFTDGACRGNPGPGGWAALLVAGAKRMEVFGAEAQTTNN
                     RMELMAAIGGLGALKRRCSVKLFTDSKYVLQGATEWLPNWKARGWRTAARAPVKNQDL
                     WQLLDAAVAAQDVEWHWVKGHSGHEGNEYVDQLANVAIDRLLAAQ"
     gene            complement(18537..19259)
                     /locus_tag="VGE92_01230"
     CDS             complement(18537..19259)
                     /locus_tag="VGE92_01230"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY1312463.1"
                     /translation="MESGRETPSSDWFDGPLAARVLREEAALAPLALDDVFGFELLQV
                     GAWGPARHLLDGARTQHTTLVAPEASCGVTLRAHLDSLPFKSDSIDAIFLPHTLELVD
                     DPYAVLREAERVLCAEGCLMICGFNPFSGWGVRRTFAKYVSRAQFPPHTQRMLSERRL
                     RDWVALLGFDVDSVYGYLGLLPTKGRAPRGVDLPKAAWRAGAYLLKARKRVPTLTQIR
                     PRRRARQVVLVGAAEPTSKVGS"
     gene            19336..20115
                     /gene="gloB"
                     /locus_tag="VGE92_01235"
     CDS             19336..20115
                     /gene="gloB"
                     /locus_tag="VGE92_01235"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEY1312464.1"
                     /translation="MHLSVRPVRAFSDNYIWLIDSPRAPGQVVAVDPGDAAPVMAELQ
                     RSGLSLAAIFLTHHHADHIGGVSALLRRGAVPVIGPDDERIPGRTLTVRDGERCELAD
                     MGLSFEILQVPGHTLSHIAFWGHDALFCGDTLFSAGCGRMFEGTPTQMNASLTKLRNL
                     PPHTRMYCGHEYTAANLRFALAVDPANGAALDYQRRVVSLRAQDQPTLPSTMELETRV
                     NPFLRCDDPKVVAAAAAHAGTAPQGGAEVFGVLRAWKDQFR"
     gene            20123..>20831
                     /locus_tag="VGE92_01240"
     CDS             20123..>20831
                     /locus_tag="VGE92_01240"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY1312465.1"
                     /translation="MTHALATRLCSVAGAMLLLAACAHQQAKTPPHEPIVVPPATTTA
                     DAPISPPDAADSAGSTPSLTMPREWQHHNGEDYDDLFDRMRAGFALDEVQEPAIDVQL
                     AWFEHNPEYLERVFQRGQRYLYHVVTEVETRGMPLEFALLPVVESAYEPFAYSGSRAA
                     GLWQFIPGTGVRFGLKQDWWYDGRRDVIESTRAALDYLQALHDQFDGDWLLAIAAYNV
                     GENTVQRELDFNRAHGKP"
BASE COUNT         3988 a         6626 c         6227 g         3990 t
ORIGIN      
        1 tcagcgatcg acgaagcgcg cgtaatagag aacgtcttgt tccacgccat cgataatctc
       61 ctgcctggtg cgcacggcct cgagatgcat ccccgagcgc tccataatac gcaccatgcc
      121 catgttgggc cgcgcggcgc cgccagtaac cttgcgcatc ccccacgacg caaacagatt
      181 gtgcatcagc gtcgtccaag cctcgagacc gtagccctga ccccagcaag ctcgatcgcc
      241 gatcaatatc cccatgtccg cggtcgtatg ctgattggaa atgtaggcgg tcatggtgcc
      301 gatcatgcgg ccgctctcgg caagcctcac cgcgagaaac acattcgtgg tgccggcaaa
      361 cgaggctatg tagtcacgcg cggattgtct cgagtgcgcg cgaaagcgtt gattgctgaa
      421 acgcatgact tcctggtcgt tcaaccatga taaatactcg tcggagacgc aggcttcatc
      481 gaatgggtgg atggcgagcc ggcgtgactt gaagctgacg ggacccggcg tcgtcatccc
      541 aggttttgca gattcgccca aaccttgtag aacgctgcga ccacgagttc aatttcggcg
      601 ggcgtgtatt catgcgcgca aatattcagg aaaaagaaat cctgttcgtg cagtctctcg
      661 gcgaccgggc agcgctgtgt agacgctctt cgcgagcaat cggcgcccaa acccagccac
      721 ggaaaaccgt ttttcccata ggcaatgcca cgctcgaaca tcggaagacg atgcacgttc
      781 tgatacccgg agccgaggcc ggtaactcct tccgcgcgca gagcggcgat gatcgcggcg
      841 cgcgggacgt tcgtgcgttc gctctgcaag cgcatgccga agacatagta gacgtgagtg
      901 cactgcgcgg agactttggg aaggatcaat ccgggaagac cgctcaaacc ctctgccact
      961 cgtcgcgccg catcttgccg cgccgcgacc ttggcagcga gttttcgcag ttgctcgcgc
     1021 gtgatggcag actcgatctc gccaaggcgg aagttgtagc cgatcatgtt ggagagctcg
     1081 gccggatctt cggatttgat cacggcctcg gcgtggttgc ggataagccg cagccgctgc
     1141 gcgtaccgat catcatcggt gaccagaatc ccgccttctc cgcaatgtat gtgcttgtgg
     1201 tagttgagac tgtagccgcc gatatccgcc agagtgccgg cgtacttgtc cttgtagcgt
     1261 gcccccggcg cctgcgcgga gtcgctcaga agcgtcagtt tatggcgggc ggctatgctc
     1321 cgcaatcgtt ccatgtcggc cgattgcccg aatatatcca ccgacatgat cgcgcgggtg
     1381 cgcggcgtga tcgccgcctc aaccgcatta ggatcaagat tgaaggattc ctcatcgatg
     1441 tcggcaaaaa ccgggatggc gttccagtgg actatcgcgg tggcggacgc agccatggtc
     1501 cacggtgtga caataatttc gtcgccgggc tccgtgccaa tcgctccgac cgcggaaatg
     1561 agacccgatg tccaggaatt caccgcaaca gcatggcgca cgccgaagta cgcggcggcc
     1621 tcggcctcga gcgcccgaac ttcgggaccg cccatgaatc cctcgccagg ggcgccgata
     1681 taagccgaca gcactccggt cttgagcacc cggtttgcag cttcaatctc ctcagcgcca
     1741 aggctccgat agggcgggaa tgctttcgcg atgacgggag caccgccgtg caaggccaac
     1801 ttttccttca tgacccgcat tgtacgttgc actcaattcc cgtggccagt gaacggcgcg
     1861 ccgcatcggc tgccgccagc gctgcgacgc catccctgcc gctgcacttt gcctgccggc
     1921 cctgacccag cgcggcgacc acatcttcga ccagtcgcag cgcgacatct ttgaatccgg
     1981 aggctatttc tccgctgggt tgcaaggtcc tataaccgga gaagtgcgga ctatcggcca
     2041 ccgaatccca ggagatccgc acgccggaat ccatcagccg cagccggccc aagctgccga
     2101 gaatatccat ctcaaaaatt gtatagcgct cctcatcgag tcccaggaga aatcccgtct
     2161 gaccgtccgc aaacatcatg cggacatgac aggtcggatc cggctgcctg ccgccggcat
     2221 ggcccgacca agcctggact cgagcgatct taccgaccat ccactgcgcc aaatcgaacc
     2281 agtgcgtgcc gttatgcatt atacccttgg tatagcaacc ttgaactgcc tgcaccatgc
     2341 caagatcgcc ggcgatcagg cggcggcgag cttcgatatg gcttggcgcg aatcggcgtg
     2401 aataattcac ggccaggatc accccgcggc tgtctgcgag ctcaaccagc gcccgcgctt
     2461 cggcaagatc cgtagccagc ggcttctcgg caatcaccgc gcgcaccccg gcatggttca
     2521 gtacttccct gagcaccgcc gcgtgcgtcg catcgggcgt gcagacgctc actatttgcg
     2581 ggcgcgtgct cgacagcagg tcggacaaat tgtggaatcc gcggcccagg ccgaagcgcg
     2641 tcgccgcggc ctgcgcgcac gcagcatccg catcgcaaac acccgccaat tcggtatccg
     2701 gacatgccgc ataggccgcg gcatgcgact ggatgccgtc ggcacgcggg tcgtccgcga
     2761 ctcccgagcc aatccaaccg caaccgacga ccacagcgcg ccacatcagc caatgagatc
     2821 ccatgacatc ggcgttccac gcggcacggt tctattgacg cgcttgccga gcaacatgct
     2881 gaggtgctgc ggcggcaagc cgttacccgg tcgaatcgcc cgcagatttt cccgggtaag
     2941 cgcatcgccc gcggccaagt cagtgcatac gtacaggctg cggcgaaaca ccagggacgg
     3001 aatttcctca gcggtcggca gggtttgtac gctgccgagt gccagccagg ccatgcggca
     3061 cgattcgacc agttgcttga gttcgggcgg ctccaaagag aatgtcgaat cgacgccgcc
     3121 tgaagcccga tccagagtta catgcttttc gatgaccgtc gcgccgcagg cgacgctggc
     3181 ggtggccacg gcgatgccca gcgtatgatc ggagagcccc acttcgcagc gataaagatc
     3241 gcgcagatag ggaatggtgc gcagattcga cgaggacgga tctgcggggt aattgctggt
     3301 gcattttaac agcaccaaat catgaccgcc cgcctcgtgc accgcccgaa cggccgcgtc
     3361 gatctcagcg agggtcgcga ggccggttga aatgatcacc ggtttgccgg tgcgagcaac
     3421 tcggcgtatc aggtcgagat cgcgattttc gaaggatgca attttgaacg ccggcacgtc
     3481 cagggattcg aggaaatcca ccgcagtgcc gtcaaacgcc gaactgaacg cggccaagcc
     3541 gtgctcgcgc gcacgctgga acagcggctt gtgccatttc cacggcgtgt gagcctcttc
     3601 gtataaggca tgcagattgc ggccgaacca gggactctcg ggattgcgga tgacaaactc
     3661 gcgttcggca acatcgatcg tcattgtgtc ggcggtgtag gtctgaagct tcaacgcatg
     3721 cgcgccgctc gctgcaatcg catcgacgat ctcgagggcc cggctgagcg agccgttatg
     3781 gttacccgac atctccgcga tgatgaacgg aggcgcgccc tcgcctacct catgcttgcc
     3841 gatgcggatc atcgttgccc acctcgatat tccaaacctc catcaaccat ggtctctcgc
     3901 catccaagct gctgaaaaat attcctgctc gcggcgtttt caggttttac gcgggcgatg
     3961 aagcggcgcg cggcacggct gcggccgaaa agcagtgccc gttctaccaa cgccctgcca
     4021 aatccgagcc cgcgcaagtc gggatcgacg ctgaaggaca atactgcctc atcgttccta
     4081 aaatccaatc tcgcctggcc ggccggaagt ccctccactt cgccgatgac catatcagaa
     4141 ttgcgatcag acagcttcgc ctggaaccat tcgaaatgcg tctgccagac gatcggactg
     4201 ctgtcaaagg acatttcgcg cgtcaacggc tcgttgcgcc aatcgaacag cagaccggcg
     4261 tcggcgatcc ttgcccgccg cagtcgcagg cgggacggca atggcggcac gatcgcggcg
     4321 cccgctcggg cgaccccgtg accgtcaacg agcggcggcg ggtcagcggc tgaacgcagc
     4381 gcgcaactga cggcggtcgt gagttcggca acggataccg aagcagcgcg ccccgcccag
     4441 atgactgcgc cggcatcggc aagatgggag accggcccct cttggtttgg cgccagcgtg
     4501 acgactatgc tcggcagtcc catgcacagg cgctctgacg tcgcaacgcc gccggcgccg
     4561 atggcaagat cagcgcgaaa catcaatccc gccagcgtcg acaagtcttt gaaaatcgcc
     4621 gtcccgggac gctgcgcagc ggcagccgca acagcttcgg gagccggatg attgacgccc
     4681 agaacgacgt cgaccgctag gtgcgcgagc tctggagctt gcagcgcgcg cagcgctttg
     4741 ctggtttcgt cggttgcatc gctggcaccg aagaaaacca gaacgcggcg cggtgcgccg
     4801 cgccgcggca cgagtgctgc gcgcagattc gcgtaatcgc gctgcaacaa cgcatagcga
     4861 ggacccagca agagtcggca atgggttgga accagaccgg cataccgatt tcccgtctgg
     4921 cagccaaaaa aattttgatc gagcagcacg tcgcagtcgt gtggtcggtc ggccaagtca
     4981 tcgatgacca cgatgcgctt gacccagcgc cgctgtgccc gctcccactc ggcatcgatg
     5041 ccgtaatgat cgatgatgag gcattcgctg cgcagctgtt ctaacacctc gcaggtctcg
     5101 gtgcgatcct gcagcttcgg cacctgaagc caattcactg cggctttgct gtccgccgcg
     5161 ccggcggatg gggtgagacg aacgaccgga taaccctcgc gctctatgtt ctctacgaga
     5221 tcgccgggca acgggcggca aatgaaagta atggcagcac cgttgcccgc cagccgaccg
     5281 gccaagctaa ggcaacggat gacatgccca gatcccatcc gcgtcccgcc atcaactcga
     5341 atcgcgatgg atctaggcgc ttcagtcgcc acgctcttcc tgctccagca gccggaatat
     5401 catttccgcc cttatccaat cttcgggcga atcgatatcc tgaacccgat tgtgcggtaa
     5461 ttcgagcatt ttcgagcgcg gaccgaatat cggccgcgcc tcgagccacg agtcgcggcg
     5521 cccgaaatag aactgcccgg cgtcatgata tgccggctcc aaatcctgcg agcgcgtctc
     5581 gcgattttcc ggccagtaca tctcgcaata gccgccggaa atcttgcgta gagcgcgctg
     5641 gatcggatac ggaaacaacg tcgccgtaaa tacgtagtcg acgtcttctg catcgagcag
     5701 atctcgcgcg ctgcggatat cgctcggacg cgacagcacc gcggcggggt acgcgcagca
     5761 aacgaatctc gcgctgcatc cgcgagattc gatctcgcgg atcgcgtgga cgatcacggg
     5821 aagcgtagct gccatatcat ttgacagctc cgccggccga cgaaatggta cttctgcgcc
     5881 ggcctgcaat gcgacgtccg cgatctcgtc gtcgtcagtg gacacgatga tgcgatcgaa
     5941 tgcgttcgcg gccctgagca tggcgattgt tcgggcgagc atgggcacgc cgacaaacgg
     6001 ccggatattt ttgcggggta tgcgtttgct gccgccgcgc gcggggatga tcgccagagc
     6061 agaccccggg ggcggttgtt cagacatggc gctcgaccac gaggttgacc gattcgatta
     6121 cgcggtcttg gtcagcatcg gtgagaccgt gccacatcgg cagcgacagc aatccggcgt
     6181 aggcgtgttc agcgaccggg cataatccgc gcgcgtagcc gagctcttga tagtaagagt
     6241 gcagatacac agggagatag tgaacgttga ctccgatatg cagcgcccgc aacgcgttga
     6301 acaccctttc cctaggctcg ccggggttct cgccggctag gcgtacggca tacagatgcc
     6361 acgaatgttt gcggtcttgc ggctcgaatt gaatacgcag caaaggcgat gactgccagg
     6421 cctctgcgta gcgagctgcg agggcgtggc ggcgttccag cgattgcggc agcctgcgaa
     6481 gctgggacag ccccagggcg cactgcacat cgttgagtcg atagttgaat ccgagatcca
     6541 ccatgtcgta ccgccaggtt ccctgctgtt cgcgtttgcg atggtcgcta tcgatgccgt
     6601 gattgcggaa gcgccgcaaa cgcaaggcaa ggtcggcatc gttggtggcg accattccac
     6661 cctcggcggt cgtgaggtgc ttcaccgggt ggaaactgaa ggcggtcatg tcggcaatcg
     6721 atcccacctt gcggccgcga tactcggcgc ccggcgcgtg acacgcatcc tcgatgagca
     6781 gcagccggtg ccgatgagcc aactcgctga gttcgtcgag gtccgcgggc aagccgccgt
     6841 agtctaccgc gacgatcgcg cgcgtccgcg gagtgatcaa cgattcgacg tgcgcggcat
     6901 ccacagtcag cgtgtcggcg cgcacatcag caaagacgat tctcgtgccc tggtaacgcg
     6961 cgcagttggc gctggcgacg aacgtcaacg ccggcactat tacctcatcg tcgctgccaa
     7021 tccctgcggc gagcatgcaa agatgcaggg cagcggtgcc gctgctggcc gtgatggcgt
     7081 agcgcgcatc cacgtaagcg gcgaaggcca gctcgaagcg ctcgaccacc ggcccggtgg
     7141 tcagccaatc gctgcgcagc acctctgcaa ccgcggcaat gtcttcgtct tcgaccactt
     7201 gccgcccata cggaatgaat ctactagtca ttgatttctt gcgcgatcag caagcgcagt
     7261 tcatcgacgg tgaggaagtt cgggttgctg ccgctgttgt agctgaagcc cggctcgacc
     7321 ggcttagcgc cgcgctcttc gcagtaggtt tcgagcgaat actggccacc tgacggcaaa
     7381 atagcatagt acctgccaag atcgacggtg ttgaaactat cgctggcagt gatcatctcc
     7441 tcgtgaatct tctcgccggg ccgaaccccg atcacgcgct gctcgcactt agaatcgacg
     7501 gcgacggcga cgtcggtgac gcgatacgaa gggattttcg gcaccaagat ttcgccgccc
     7561 caggcatgct cgattgccca cagcaccatg tccacgccct cttgaactga aatattgaat
     7621 cgcgtcatgt tggggtcggt gatcggcagc acccccgaac tcctcttgtc gaggaagaac
     7681 ggaatgacgg aacctctgct gcccatgaca ttgccgtatc gaaccacact gaatcggatg
     7741 tcgcggctgc ccttgatgtt gttggccgcg atgaacagct tgtctgaaca cagcttggtg
     7801 gcaccataaa gattgatggg cgccgccgcc ttgtcggtcg atagggcgac aacacggctc
     7861 acgccgctgt ccaagcaggc atcgatcaga ttctgcgcgc cgagcacgtt tgtcttgata
     7921 cactcgaacg gattgtattc ggcagccggc acctgcttca gagcggcggc gtgcaccacg
     7981 atgtcgatcc cctcgagcgc gcggcgcaag cggccctcgt cgcggatgtc gccgaggaag
     8041 tagcgcattc ccttgtgttg ccgatcggaa aatacctgcg ccatctcgaa ttgcttgagt
     8101 tcatcgcgcg agtagacgac cagacggttg aattccggat ggctgcgcag taccgacttg
     8161 acgaaagccc tgccgaatga cccggtgccg ccggtgacga gaatcgattt gttgctgaac
     8221 ataatatctt cagtgcgtca cgaccgccgt ccgcgggcgt gaaccgtcgg taacaagcgc
     8281 gaaaataacc ccaaccatga ggcccaaggt aaatcccgcc gcgatcatcg tgcctttctg
     8341 cggcttgatc ggatcatcga catccgcggg catggcccgg tcaaccacgc gaaagacgaa
     8401 ctccttgctg acgttcgcga gcattcgctg cttcacctgc gactcgatca aatgtccgat
     8461 cgcatcgtgc gcagtcacca ccgttgtcgt ctgcagttcc ttatcgagat accccaggga
     8521 ggcatcggca tcggaaatcg cgcgggaacg catctcctga ttcagctgcg ccactagatc
     8581 gttggtccac ttggccgcct cgtttcggtc ccgccagtcg atctggacgg agatcagtcc
     8641 ggtcttctta tcttgatcga cggagcgaat tttttgattg aagtatcgat acgccttatc
     8701 gagcgtcggc ggttcgccct tccattgatt gcttgctgcg tcccatttct tgaagaacag
     8761 ccgagtcatg atccgctcgt cgacgatgaa gcgctcggtg aactcgcgcg agcgcaacac
     8821 ggccagcgcc tcctcggtca cggaagtgcg ggaaccgacg ttgaggccgg cgagcgaggc
     8881 gatgcctcct aggtcgccga gcgcggaacc gaggccgccg ctaccctggt tcgcgttgga
     8941 cgccaccaag atcgtcgtgg atcgatagat cggcgtcatc acgaacgcgg cagcgccgaa
     9001 cgcagcggtg aaaaatagca cgctccccaa cagccaccag cgcttggcgc gcaacgcggc
     9061 gatgatgcgt cgcgaatcga tggtgtcctc atcctcgaac acagctgcgg gcgttttcat
     9121 cggacggaat tacacactgc gaaccgccaa aagcgccacg gccaagttgt aaatgatcgt
     9181 cgtcacggcc tgccacagcg gcaaggcgcg gatccgctcg gtgtcgaggg gaacaacgat
     9241 ggtgtctccc ggccgcatct cgaccgactg cgagcgtctg aaccaacccg agcgctcgcc
     9301 ggaactgacg tcgccgttcg cgcgcactac gtaaatgcgc ttgcgatccg ccttctgcgt
     9361 ggtgccgccc gaacgggcaa tgtaatcgtc gcgagtgagc ccggcttcga acacatgcga
     9421 agtggggctc tggacttcgc ccaatatggt gatttcctgg gttttcttcg ggatgagcag
     9481 cttgtcgccg tcgcgcagcg caacgtcctc ggaaccgccc gccggacgct tgaccacccg
     9541 attgatgtca atcaccagcc ggccgaccgg cttggtatcg cgcaattgat tcatcagttg
     9601 ttggccaaca gccaagcctt gagccgcgcc gttgccgctg ccggaattgg tggcggcact
     9661 gccggtgatc gcttccagcg agagtgcggc cagatcgctc tgcagtcggt tcgccaataa
     9721 ttcgagctgg tccttctcgc gcttcttcaa ttcctcacgg atgaatactg cgccctcgat
     9781 gaacgccagg tcggtaaatc cgccagcccg ctgtaagact gaggagagcg tctcgccttg
     9841 gtggatgggg tacttgccgg ggaatcggac ttcgcccgcg agttcgatga cgccgggctc
     9901 gacccattgc ggaatgggct tgatgatcag gacgtcatag ggcttcagca acagatcggc
     9961 cccggcgtcg ccgcgccgta tggccgcaag atcgaccgcg atcaattccg tcttgcgcgc
    10021 atcgcccggc gtgacttcgt agcgagtaat ttccgcctcg ccgcggaatg ccgcatcttc
    10081 taggctgcca ccggcgcgaa tcaaatcgct gacatgcatg gcgggctcga gcgggtagtg
    10141 ccccggcgcc ttgacccggc cgtcgatact gacgacctgc tccggctgct cgggcgtcgc
    10201 ctgcagctgc aactgccgaa tcaccggtcc cagaattcgt tcgcggtccg tcgacaggtc
    10261 gaatacatag attttatccc gcggccgcaa ctcggggtcc gccgcagaac cgcgcgccgc
    10321 gagtgcgcgc tgcagatcgg cggacagcac ttgcacggtt tcgttgggcg gcacttcgcg
    10381 ccggatcatg atgtagcgct ggtcagcgtt gggccgcaat tcgtcgaagc tagtcaatat
    10441 atcggtgagc cgaagccccg ggtgatattc aaattgcccc ggtcgaaaca caaatcccgt
    10501 gagcaccacc gaattttcga gcgtcggccg aatcgcgggg attctcaatt tatcgccgtc
    10561 cgcgatttcc atgccgcggc ctgccggctt ggtcagatcg atgttgcgca tctcgtgcag
    10621 gcgcgacggc agaattcgct cgagttggcc gagcttctcg tctgcatcgc tcagcaggcc
    10681 gccagcaatt tcgatggcct ggcctacggt cttttcgcct ttgagctcat aaatggccgg
    10741 gcgccgtacc gctccatcga ccgccatcgt gcggccgatg gggggaatga aaatgacgtc
    10801 gccgggcagc aactgccggt ccccgctggt atcgccatgg agcagcaagt cgtacaggtc
    10861 gagagtcgtg accaagcgtc cgttgcgctt cagctcgatc gtgcgtagtg agccgatttt
    10921 cttgactccg ccgctgacga acaacgcgtt cgtcatggtc gacaggccgc tgacggtgta
    10981 tgaacctggt ttttccgcct cgcccaaaac gaatactcgg atcgagcgca gatcgcccat
    11041 cgtcacgctg acgcgcgtgc cgatgagctg cttggccacg cgctgctcga tagtttcgcg
    11101 ggcggcgtcg aaactcatgc cgctcaccac gatgggtccg agctttggga aattcagctt
    11161 gccgtcgcgc tcgacggtca attcgtagcg cgaggactcg ttgccgtaga gttgaatgtt
    11221 gaacgtgtcg cccggtccca cgatgtaatc ggctggaacc tggatgtcgg agacaggcgc
    11281 aaaggtgctc gggactccct ggaacaagtc atagccgaat ggcttcaatg cgtcattgcc
    11341 agagggcatc aggcgcagca aggtcaagcg cacggcaaag tcggcgagat ccgggtcggc
    11401 gcccagccgt ttggttgctt cggaggccgt caacccggcc agcgggatcg cgggcaagcc
    11461 gggcatttcc aacaccccga agccgttcag ccggtacggg ttgttgtcga gaatccgctt
    11521 gcggaatttc tcggttctct ccttttccaa gtctgtcttg ggcactggca tcggccggca
    11581 aaacgtattg ctaaaattgt tgttcggagc attgccggtc gcgccgcgca cggaagtgcc
    11641 ggtggttgcc ccgagagcac cggcggcgcc gttgacgccg ttgaacaccg gaatgccgtt
    11701 ggcgccaatc gcgccaatcg cttgattggc attgctctgc ggattgttgt tattgttctg
    11761 ctccgcgttc tggttgggtt tggcgttgca caggtcatcg ttcgagatca attcgattac
    11821 gacggtgtct tcgccgcgca gctccggatc ctcgtccgac tgccgcaggg tgcgcccgtc
    11881 gagcgtcctc tgtgcgcgct ctttttccgc ctgatcgccg ttcgcgcgcc gcaccgtgtc
    11941 gggcgtatcg agcttgggat cgaccttctt ccccgagccg gcgccctttc ctcctaacac
    12001 atcctgcagg atggagcctt gttgatcggg cgacagggag ttcttgaggg cctgcaaagt
    12061 atctgaattc tggctctgcg tcgaccccac caagccgatc gacagcgccg cgagcaacgc
    12121 gcctagcgat ctagtccgca tgagtcatga tctcgatacc atctcttcaa caacctcgat
    12181 tgccaatctc ggtgcacatc atagccttta ccgccatcgc ggtaacctcg ctgcaggcgg
    12241 acatgcacgc tactgcttgt agttcttatt tagccaatcc gcgtaatcgc ggcccaataa
    12301 cgccttccac cagccgatgt tggctaagta ccaatctagc gtggcaccca aaccgttcac
    12361 gagattgccg gccggagcgt agcccaattc tcgcgctgcc ttcgcgtagt cgatggcata
    12421 gcggcggtcg tgccccggcc gatcgcgcac atgggtgatc aaatcgaccg cgcgcttgcc
    12481 ctgcacagcc ggtgaagccg gaaacgccgc gcgcaagtcc gcgcgctcgc caagcgcctt
    12541 gtccgttaga gcacacagcg tacggactat ctcgatattg gtcgtttcgc tatctccgcc
    12601 gacgttatac acctcgcccg actttgcgcg ccgcagcgcc agcgcgatcg ccacgcagtg
    12661 atcttcaacg tgaagccaat cgcgcacctg caacccatcg ccgtagacag gtaatggctt
    12721 acccagcaaa atgttaacga tcgtgagcgg gatcagtttc tccggaaatt gataaggacc
    12781 atagttgttc gaacaattgg tcacggttgt gactaaccca taggtctcat ggtaagcccg
    12841 cactaaatgg tccgaagacg ccttgctggc ggagtacggc gagttagggg cgtacggggt
    12901 cgtctcacgg aacggagcat cttgcggccc gagcgatccg tacacctcgt cggtcgagac
    12961 gtgatggaag cggtgcgcag ccacggtttt tcggtctatc cacagagttt ttgccgcttt
    13021 cagcaacgag tgagtgccga cgatgttggt gcgaatgaaa tcgtcggggc cgagaatcga
    13081 ccgatccacg tgcgactctg cggcaaagtg agcgatagta tcgatctgat gctgctcgag
    13141 cagcgcacgc accgtggcct catcgcaaat atctcccttg acgaaaaggt agcgcggatt
    13201 ctgatcgagg cccgcgaggt tttcgatatt gccggcatat gtgagcgcat cgaacaccac
    13261 cacccgcccc tcgctggtgc tcgacaacca gtggcgggcg aagttggcgc cgataaaccc
    13321 ggcgcctccg gtgatgaaca ggttatgtgg ttcgtagctc atccagcata gtcctcaaat
    13381 tatgtcgcca atagcgtgcc ggcaccttga tcagggtccg tgtcgacacg gtatcgagca
    13441 cgctgaacgc aggccggcgc gcgcgcgtcg gatacgcggc gctgcgaatc ggattgacgg
    13501 gtacggcgcg gctgagtaag ccgcgcgcca aggcttcatc ttgaattgcg accgcaaagt
    13561 cgtaccagct cgctactccg agatcggtcc aatggtagat gccaccgggt gcgccaattt
    13621 cgatgagtcc ccatattgcc gcagcgatgc tccctgccca ggtcggcgtg ccgatttggt
    13681 cgctaaccac gcttacctgc tctttctctt gcatcagccg cagcatggtc agcacgaagt
    13741 tttttcccgc agcagcgtat acccacgcgg tgcgcagtac gatgccggtc gcgccgcgga
    13801 gcgcctgccg ctcgccacca agcttgctcg tgccgtagac gctcaaggga ttaggttggt
    13861 ccccgggcaa gtacgcgcgg ttcgactggc cgtcgaacac gaagtccgtc gataaatgca
    13921 gcaatcggca ggccgacgcc gtagcggccg atgccaatac gcccacggcg gtgtcgttga
    13981 cggcaatcgc ttgagcggga tgatcctcag ccagatcgac cgccgtgtag gcggccgcat
    14041 tgatgatcca ttgcgcgcgc gtttccgcca atgcatctgc cactgccttt tcgtcgccga
    14101 tgtcgagttg agcgcggctg cgtaccagga cctcgcgccc ctcgccggcc gccgcaacga
    14161 ctgcgtttgc cacctggccg ccgccgccca acaccagcac cttcacgcga acttctcaat
    14221 ctcggcaaag ctcgcaccgc gcgcatcctt ggcggacaat ttgggagtca cgcccgccgg
    14281 aagcggccag cggatttcga gcgtcggatc gttccaagcg agggtgcgct catgctccgg
    14341 gctgtagaag tcggtgcatt tgtagaggaa atcggccgat tcggacgtga tcaaaatgcc
    14401 gtgcgccaag cccggcggca cccacaagat ccgatgatta tcggcggaga gctccactcc
    14461 ccacgacttg ccgaaggttg ggctgctgcg ccgcagatcg accaccacgt cgaatacgct
    14521 gccgagcgcg acgcgcacca gcttgccttg agtgtgctgc acctgcaggt gcaatccgcg
    14581 caatgtccat tgactcgagc ggctgtggtt gtcttgcaga taggtcgcgt ccagaccggc
    14641 ggctttgaat ttggcttcgt gccacgactc catgaagaat ccgcgcgcat cgccgaacac
    14701 ctgcggttcg atcagcgtta catcggcgat gggagtcttg gtaaatttca tgcggccgaa
    14761 ttcttcacag ctgcgagtgc tcgttgacga tgcgcagcaa atactcgccg tagccgctct
    14821 tggcgagggg cttggcgagt ttgaccaaat cctcggcgct gatgaacccc gagcgccaag
    14881 cgatctcttc cacgcaggcc accttcagcc cctggcgctc ctcgatgatg cgcacgaaca
    14941 cccccgcgtc gagcaatgag ttgtgcgtac cggtatcgag ccatgcggta ccgcggccga
    15001 gccgctcgac gtgcaggtcg ccgacttgca ggtaggcgcg attcagatcg gtgatttcca
    15061 gctcgccgcg cggcgagggc ttgagggcct tggcgaacgc gggagcgcga ccgtcgtaga
    15121 aataaatccc ggtgaccgca tagttcgacg gcggcaactt gggcttctcc aataaatccg
    15181 tgacgcggcc cgcggcatcg aactgcacca cgccatagcg ttctggatcc tgtacgtagt
    15241 agccgaacac ggtggcgccg gattcgcgct gcgccgccga catcaatagc gcggtgaggt
    15301 tcgcgccgta gaatatattg tcgccgagaa tcagcgctga cggcgcaccg tcgagaaacg
    15361 actcgccgat gatgagcgcc tgcgcgatgc cgttcggcac cgcttggacc acgtactcga
    15421 acgacaagcc ccactgcgcg ccggtcccca gcagatcgcg gaaggctccc tggtcgcgcg
    15481 gtgtggtgat gatcagatac tcgcggatgc cggcggacat cagcgctgcc agcggataat
    15541 aaatcatcgg cttgtcgtac accggcaaca actgcttgct gagcgacatc gtgatcggat
    15601 gcaggcgggt gccggcgccg cctgccagaa tgatgccctt tcgtggtttc accgagtcgc
    15661 tcgcaattcg tttcgaggcc ccggattata gggtatcgcc atgcatcgca tccgggataa
    15721 ttgcgccgtt gcgaggcccc tagcgatgcc gcgccacata gtcgatcagc gaacccaacc
    15781 cggccgctcc gtgccccgcc ggcgcgcgcc cgcccttcat gtcgattcct accgcgagtt
    15841 tcttgcccaa ctcgacgccg aactgatcga aggagttgat gccccaaacg gtgccttgaa
    15901 cgaacacact gtgctcatac aaggccagca gtccgcccag gcttcgcgcc gtggtctgcg
    15961 ggtagagcaa cagggagctc gggcgattgc cgtcgtaaac ccggtgagcc ccgatccgcg
    16021 caatctcctc gggcccggca ccggcgcgtc gcatgtcggc ctggacttct tccaaggagt
    16081 gtccaaaggc aaatgcctgc gcttgcgcaa agcaattctg cagcgccaga tcctgtgcct
    16141 cggtacctcc ccacgaaccg cgcagcggtg cgataaaatc caaagcctgc gtcagcgttc
    16201 cttgatgcaa catctggaag aatgaatgct gcgcgtttga gcccggttcg ccccagacga
    16261 tgggcgcggt ggcgaaatcg acgcgtccgc ccgccaagct caccgacttg ccgttgctct
    16321 ccatttcgag ctgctgcaga taagcgggta atctcgccag gcgttgatcg tagggcagca
    16381 cggcgagggt cggcagctcg aggaacgtcc ggttccacac ccccagcaac cccatgagca
    16441 ccggcaggtt cttctcgaac ggcgcttgtg tgaagtgccg gtcgatgtcg ctcgcgccgc
    16501 tcaagaactc ctcgaaacgc ccgctgccga cgctgagctc cgcgatcaga ccgatggcag
    16561 accacaccga ataacggccg cccacccaat cccacatcgt aaagcgatga tcggcggcga
    16621 taccgaatgc atccatggcg ggcgcattcg tcgataccgc gacgaagtga ttcgccggcg
    16681 acttctcgcc gagcgcgccg acgacccagg cgcgcgccgc gttggcgttg gcctgcgttt
    16741 ccaaggtcgt gaaggtcttc gagcagacga tcaccagagt ttcagccggg tcgatgctgc
    16801 gaatcagatc ctcgagctga gtgcggtcga cgttcgacac aaagtgcggc gtgatatcgc
    16861 cgctccactc gtgcctgagc gcttcgcaga ccagcaacgg acccaagtcc gaaccaccga
    16921 tgccgatgtt gacgacatgt ttgaatttct tgccggtgat cccgagtttg cggcccgaac
    16981 gcaccgccgc gacgaaagcg accagctgtt tgcgggactc gcggacctcg gactgaatct
    17041 gcgcggaacc ggcgaaatcc gagcgcagcg cggtgtgcag aaccgcccgc tgctcggtgc
    17101 tgttgatcaa gtcgccgcga aacatcgcgg ccatgcgtgc cggcagctgc gccgcgcggg
    17161 ccagatcgat caacagcgac agggttttgc cggtgattcg ttgacgcgac atgtcgagca
    17221 gccaggcatc gtgctcgacg tgaaagttct gccaacgctg cggatccgct gcggtcagat
    17281 cgcgcaaatg cgcgccggca atgtcgccgg catgggcagc cagcgcgtgc cactcagcca
    17341 gagaagagtt gatctgtgtc atgtcacacc cgtttcggag gattcatttc tgcgccacac
    17401 cgtcctgccg cccgacgcct tgtcgagcaa cgccagcacg tgttcatgcg cgtccatctc
    17461 ttgctcgctc ggccgcacca cgatgatgcg cacggccgcg ttgagtcgcg ccggcgcttg
    17521 acgcgcgcgg ctgcgcgcgg tttccgattc ggcgctgagc gacaacatgg cctggccgcc
    17581 ggtcatggcg aggtacacct cggctaggat gcgcgcgtcg agtaaggcgc catggtaatc
    17641 gcgatgcgag ttgtcaacgg aatagcgctt gcacaaggcg tcgaggttgt tgcgctgccc
    17701 gggatgcatc tgtcgcgcca tcgccaacgt gtcgagcacc cggcacagat catcgacccg
    17761 gtgcgcgaca tcgatgagag ccagttccgc attcaagaaa gcgatgtcga atggggcgtt
    17821 gtgaatgatc agttccgcgt cgcggatgaa ctccaggaac tgcggatgga cctcggcgaa
    17881 gctcggctct cttgccaatc gttcgcgggt caatccatgc accgcttgcg cgccctcatc
    17941 gatgtcgcgc tgcggattga gatattgatg gtaggttcgg ccggtcaagc gccggtgcac
    18001 cagttccaca cagcctattt cgatgacccg gtgtttttgc tcgacttcca ggcccgtggt
    18061 ttccgtatcg agtacgactt gacgcattgc cgcagactac tgcgccgcca gcaggcggtc
    18121 gatggcgacg ttggcgagtt gatcgacata ttcattgcct tcgtgccccg aatgtccctt
    18181 cacccaatgc cattcgacgt cctgcgccgc aaccgcggcg tccagcagct gccatagatc
    18241 ctggttcttg accggcgcgc gcgccgccgt gcgccagccg cgcgccttcc agttgggcag
    18301 ccattcggtc gcgccttgca gcacgtattt cgaatcggta aagagcttga ccgagcaacg
    18361 ccgtttgagc gcgcccaagc cgccgatggc cgccatcaat tccatacggt tgttggtggt
    18421 ttgggcttcc gcgccaaaaa cttccatgcg cttggcgccg gcaaccagca gcgctgccca
    18481 gccgccgggc ccggggtttc cccggcatgc gccgtctgtg aatatgtcga cctgggtcat
    18541 gatcccacct tcgatgtggg ttcagcggca cccaccagga ccacctggcg ggcgcgccgt
    18601 cgcggccgga tctgcgtcaa ggttggtacc cgcttgcgcg ccttcaagag ataggcaccc
    18661 gcccgccagg cggccttcgg tagatcgacc ccgcgcggcg cccgcccctt cgtcggcaag
    18721 agcccaaggt agccataaac gctgtcgaca tcgaagccga gcaatgccac ccagtcgcgc
    18781 agccgacgct cggacagcat gcgctgggtg tgcggtggaa attgcgctcg gctgacgtat
    18841 ttcgcgaacg tgcggcgcac cccccagccg ctgaacggat tgaagccgca aatcatcaag
    18901 cagccttccg cgcacagcac acgctcggcc tcgcgcaata ccgcgtaggg atcgtcgacc
    18961 agttcgagcg tatgcggcaa aaatatcgcg tcgatggaat ctgacttgaa aggcaaggaa
    19021 tccagatgcg cccgcaaggt aacgccgcaa ctcgcttccg gcgcgaccag ggtcgtatgc
    19081 tgcgtgcgcg cgccgtcgag caaatgccgg gccggtcccc atgcacccac ctgcagcagt
    19141 tcgaaaccga acacatcgtc caatgcgagc ggcgccaagg ctgcctcttc gcgcagaacg
    19201 cgcgcagcca gcggcccgtc aaaccaatcg ctcgagggag tttcacgccc tgattccaac
    19261 cacggctcct tcacgaaaaa ctagcgaagt tcgaatgtta caggttaaat acgccgcaaa
    19321 atgtcaattc ctagcatgca tcttagcgtg cgcccggtgc gcgcattctc cgataactac
    19381 atttggctta tcgactcgcc gcgagcgcca ggccaagtcg tggcggtcga cccgggcgat
    19441 gccgcccccg tgatggccga gttgcagcgc agcggcctaa gcttagccgc cattttcctg
    19501 actcaccatc acgccgatca catcggtggc gtgtcggcgc tcctgcgccg gggcgcggtc
    19561 ccggtgattg ggccggacga tgagcgtatt cctgggcgta ccctgacggt gcgcgacggc
    19621 gagcgctgcg aattggcgga tatgggactt tccttcgaga ttttgcaagt ccccgggcat
    19681 acgttaagcc acatcgcgtt ctggggacat gacgcgctgt tttgcggaga tacgttgttc
    19741 agcgccggtt gcggccggat gttcgaaggt acgcccacgc agatgaatgc ttcactgacc
    19801 aaattacgca acctgccgcc gcacactcga atgtactgcg gccacgagta tacggcggcc
    19861 aacttgcgct tcgcgctcgc cgtcgacccg gcgaatggcg cggcgctcga ctaccagcgg
    19921 cgggttgtca gcctgcgagc ccaagatcag cccacgctgc cgtcgaccat ggagttggaa
    19981 actcgcgtga accctttttt gcgttgcgat gaccccaagg tcgtcgctgc cgccgcggct
    20041 cacgccggaa ccgctcctca aggcggagcc gaggtgttcg gtgtgttgcg cgcctggaag
    20101 gatcagtttc gctagccgtt gtatgaccca cgccctcgcc acccgccttt gttccgttgc
    20161 cggcgcgatg ctgctgctcg ccgcctgtgc gcatcagcag gccaagacgc cgccgcacga
    20221 acccatcgtg gtaccgcccg caacgacgac cgccgatgcg cccatctcgc cgcccgacgc
    20281 cgccgattcc gccggctcga cgccctcgct cacgatgccg cgcgaatggc agcatcacaa
    20341 tggcgaggac tacgacgatt tattcgatcg catgcgagcc ggctttgccc tcgacgaggt
    20401 gcaggaaccc gcgatcgacg tccaactcgc ctggttcgag cacaacccgg agtatctcga
    20461 gagggtattc cagcgcggcc agcgctacct ctatcacgtc gtcaccgaag tcgagacgcg
    20521 cggcatgccc ttggaattcg cgctgctacc ggtggtggaa agcgcctatg agccgttcgc
    20581 ctattcgggg agccgtgccg ccggcttatg gcagttcatt ccgggcacgg gcgtacgttt
    20641 cggcctgaag caggattggt ggtacgacgg ccggcgcgat gtcatcgagt cgacgcgcgc
    20701 ggcgctcgat tatctgcaag ccttgcacga tcaattcgac ggcgactggc tgctcgccat
    20761 cgccgcctac aacgtcggcg agaacaccgt gcagcgcgag ctcgacttca accgcgcgca
    20821 cggcaaaccc g
//
LOCUS       DASWZT010000048         6571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_39212, whole genome shotgun sequence.
ACCESSION   DASWZT010000048 DASWZT010000000
VERSION     DASWZT010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6571
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39212"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..1596
                     /gene="radA"
                     /locus_tag="VGE92_01245"
     CDS             241..1596
                     /gene="radA"
                     /locus_tag="VGE92_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEY1312466.1"
                     /translation="MAKSKEVFICQNCGVSSPKWQGQCAGCGEWNSLVAEVSRINPRK
                     SASPSRAPRADHSSSLAAEAQLDEPRLATGSTELDRVLGGGLVPGSVTLIGGDPGIGK
                     STLMLQAAAALNDSGAVLYATGEESLKQVALRARRLGLASATARLITETCVEDIVAAA
                     ATLAAQVLIVDSIQTMHSERVESAPGAVSQLRECTAELVRFAKASGTAVLLVGHVTKE
                     GQIAGPRVLEHMVDTVLYFESDTGSRFRVLRSVKNRFGAANEIGVFAMVEQGLREVTN
                     PSAIFLSRHEQPVPGSVITVMREGTRSLLVEVQVLADASLGANPRRVAVGIDGNRLTM
                     LLAVAHRHAALQLQGQDVFANVVGGVRLAETAGDLAIVMAARSSLRDMPVPNSLIAFG
                     ELGLAGEIRPVPFGEERLREAAKHGFKLAVVPEANVPRRPPEGMSVRGVTRLNQALDA
                     F"
     gene            complement(1597..2868)
                     /locus_tag="VGE92_01250"
     CDS             complement(1597..2868)
                     /locus_tag="VGE92_01250"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEY1312467.1"
                     /translation="MPHPTLGVLFGALAGLLLLAAFFAGSETALMSVNRYRLRHSANE
                     GNRAARLAEKLLAHPDRLIGLILLLSTLVNVAAPMLVGFIALRLGGEILVAFGAGALT
                     LVLLIFCEVAPKTFGALHPERLALPAAYIYTPLLMVLYPFVWATNLLANGVLRLFGVS
                     SQQASNSLSSEELRTVVAEAGAMIPHRHQQMLVSILDLESATVEDIMVPRNEIVGIDV
                     DDDWDRVLEQLRQSQHTRLPVYEGEIDRIIGVLHMKQVVHELARGRLDRASLMEAAAA
                     RDAYFVPSGTTLNTQLLNFQRNKRRMAFVVDEYGDIQGLVTLEDILEEIVGEFTTDPA
                     TMMHKDVHAEADGSFVANASATIRALNRSMRWNLPTDGPKTLNGVIVEFLETIPEVGT
                     TLKLADYMLEVLQTGDNAIKTVRIRPPTPGV"
     gene            complement(2878..3669)
                     /gene="ccsA"
                     /locus_tag="VGE92_01255"
     CDS             complement(2878..3669)
                     /gene="ccsA"
                     /locus_tag="VGE92_01255"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEY1312468.1"
                     /translation="MILAITLFLLYAASAWLMLRSVNKPRLEPIAWALSLASIVAHSD
                     AIVHMIRVNGSFAIGLLEAISLLAWTLGVLACFISIERQNRVLGALLFASAAFGAAAT
                     DAGHGYVEAAVPGWELTAHILLAMGAAALLFAAAVTALLLVFLDRRLRTRRLANLPSA
                     IPPLDVLEKVMFRLIGTGFIMLTLALVTGFVFVTNLFTQNLTQKTLLSLIAWTIFGVL
                     LIGRVRFGWRGRSATGWTLSGFGVLALAYFGAKFVLEDLLGRHWG"
     gene            3777..5141
                     /gene="ffh"
                     /locus_tag="VGE92_01260"
     CDS             3777..5141
                     /gene="ffh"
                     /locus_tag="VGE92_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY1312469.1"
                     /translation="MFDRLTTSLAAALKSLSGRGRLTDENISETVREVRLALLDADVA
                     LGVVKTFTDAVKARAVGVEVTKSLTPGQAFIKIVHEELTRLMGEPSTGLNLRAQRPVV
                     VLLAGLQGAGKTTSAGKLARWLIEKQNKKVLMVSTDVYRPAAILQLQTLAAQVGAGFA
                     PALASEAPVAIAKRALVEATLQAYDVLLLDTAGRLHIDAEMMTEVRELEAALSPHERL
                     FVVDSMAGQDAVNAAKAFNDALSLTGVILTKADGDARGGAALSVRQVTGKPILFVGTG
                     EKSEALELFQPERIASRILGMGDVLSLVEQVQGQVDQEKAEKLAKKISKGKSFDLSDL
                     RDQLSQLQNMGGMSALLDKLPAQLQANAAAAAGAVNPKTLKRQMGIIDSMTPRERRRP
                     ELIDGSRKRRIAAGAGVQLPEVNRLLKQFDHMQKTMKKLAKGGMFKNMMRGMAGRLPP
                     GFGP"
     gene            5257..5508
                     /gene="rpsP"
                     /locus_tag="VGE92_01265"
     CDS             5257..5508
                     /gene="rpsP"
                     /locus_tag="VGE92_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010630215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEY1312470.1"
                     /translation="MVTIRLTRRGGKKTPFYHVVVTDSRKRQGGTSLESVGLFNPIAR
                     GAEVKLRLDVARIEHWLSKGAHMSERVKYLLTSFRKQAA"
     gene            5527..6045
                     /gene="rimM"
                     /locus_tag="VGE92_01270"
     CDS             5527..6045
                     /gene="rimM"
                     /locus_tag="VGE92_01270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEY1312471.1"
                     /translation="MSDESSALIQLGFVGAPFGVRGWIKLRSHTDPPERLLDHRQLTI
                     GQADAWRSYRIEASGRSGGALTVKLAGVEDRDQAQALRGAQVCVARSELPPRDARDFY
                     RADLIGCEVVNLDGKDLGSVRHFVETPAQVLMVVRGTQEYWIPAVPQHLRRVDLEARR
                     VVVDWDACALPS"
     gene            6027..>6571
                     /gene="trmD"
                     /locus_tag="VGE92_01275"
     CDS             6027..>6571
                     /gene="trmD"
                     /locus_tag="VGE92_01275"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEY1312472.1"
                     /translation="MRIAVVTLFPAMIREALTHGVVGRALERGVLEVECFDPRDHATD
                     VHRTVDDRSYGGGPGMVLKVEPLRSALRAALAAVPHGSRRVYLGADGVRFDQALARAA
                     CDGAGLVLVAGRYEGVDERFIERETDEQWSIGDYVLSGGELPALVVIDAIARLIPGTL
                     GSAESAQQESFSDGLVDWPHYT"
BASE COUNT         1266 a         1988 c         2209 g         1108 t
ORIGIN      
        1 gcgtgagcgc gcagccgccg gccaatagcg ccatgcccgg aatccacaac caggtgtcca
       61 aattcgcgat taggcgaagc aaaagaccca atactacggt ccccaaaccc acaatcgctg
      121 cgatcagccc caatttgcgc atgcaataga cataatccca attcgacaag cccggcagta
      181 taggtgatcg ggccgcatta gcagcaatgc taagctcaca aatcgacgcg aaggaagcca
      241 atggcgaagt ccaaagaggt ctttatttgc cagaactgcg gagtatcttc gccaaaatgg
      301 cagggacagt gcgccggttg cggcgaatgg aacagcttgg tggccgaagt ctcgcgcatc
      361 aatccgcgca aatccgcctc accgagccgt gccccgcgcg cggatcattc ctcctcgctg
      421 gccgccgagg cgcaactcga cgaaccgcgt ttggcgaccg gttccacgga actcgatcgc
      481 gtcctcggcg gcgggctcgt gccgggatcg gtgaccctga tcggcggcga tcccgggatc
      541 ggcaaatcga cgttgatgct gcaggccgcc gcggcgctca atgactcagg agcggtgctg
      601 tacgcaaccg gcgaggaatc tttgaagcaa gtcgcacttc gggcgcggcg cctggggctg
      661 gcgagcgcca cggcacgcct catcaccgaa acctgcgtcg aggacatcgt cgccgccgcg
      721 gccaccctgg cggcgcaggt gctgatcgtc gactcgattc aaaccatgca ctccgaacgg
      781 gtcgagtcgg cgccgggcgc ggtctcgcaa ctgcgcgagt gcaccgcgga actggtgcgt
      841 tttgcgaagg cgagcggtac ggcggtgctg ctggtgggcc acgtcaccaa ggaggggcag
      901 atcgccggac cgcgggtgct cgaacacatg gtcgacaccg tgctctattt cgagagcgat
      961 accggcagcc gctttcgggt gctgcggtcg gtgaaaaacc gcttcggcgc cgccaacgaa
     1021 atcggcgtgt ttgccatggt cgaacagggg ctgcgcgaag tcacgaatcc gtcggccatt
     1081 ttcttgtcgc ggcacgagca gccggtgccg ggcagcgtca tcacggtgat gcgcgaggga
     1141 acgcgttcgc tgttggtcga ggtccaggtg ctggccgacg caagtctcgg cgccaatccg
     1201 cgccgggtgg cagtcggcat cgacggcaat cgcctgacga tgctcctggc cgtggcgcat
     1261 cgccatgcag cgctgcagtt gcaaggacag gatgtgttcg cgaatgtcgt cggcggcgtg
     1321 cgcttggcgg agaccgccgg agacttggcg atcgtgatgg cggcgcgctc gagcttgcgc
     1381 gatatgccgg tgccgaactc gttgatcgcg ttcggtgaac tcgggcttgc cggagaaatt
     1441 cggccggtgc cgttcggcga ggagcggctg cgcgaggcgg ccaagcatgg cttcaaattg
     1501 gcggtcgttc cggaggcgaa tgtgccgcga cggccccccg agggaatgtc ggtgcgcggc
     1561 gtgacgcggt tgaaccaagc gctcgatgca ttttgactag acgccgggcg tcggcgggcg
     1621 aatgcgcacg gtcttgatgg cgttgtcgcc ggtttgcagc acttcgagca tgtagtccgc
     1681 caacttcaag gtcgtcccca cctcggggat ggtctccagg aattccacga tcacgccatt
     1741 caaggttttc ggcccgtccg tgggcaggtt ccagcgcatg gagcgattca gtgcgcgaat
     1801 cgtcgccgac gcattggcga cgaaactgcc gtcggcctcg gcgtgcacat ccttgtgcat
     1861 catcgtcgcg ggatcggtcg tgaattcgcc gacgatctcc tcgagtatgt cctcgagcgt
     1921 caccaggcct tgaatatccc cgtattcatc gacgacgaac gccatgcggc gtttgttgcg
     1981 ctgaaaattc agcagttggg tattgagagt ggtgcccgac ggtacgaaat aagcatcgcg
     2041 cgcggcggcc gcttccatca ggctcgcgcg gtccaagcgg ccgcgggcca gctcgtgcac
     2101 gacctgcttc atgtgcagta cgccgatgat gcggtcgatc tcgccttcgt aaaccggcag
     2161 gcgcgtgtgc tggctttggc gcagctgctc gagaacccga tcccaatcat cgtccacgtc
     2221 gatgccgacg atttcgttgc gcggcaccat gatgtcttcc acggtggcgc tttcaagatc
     2281 gaggatgctg accaacattt gttggtgacg atgcggaatc atggcgccgg cctccgccac
     2341 cacggtgcgc aattcctcgc tgctcaggga attcgaggcc tgttgcgagg ataccccgaa
     2401 caggcgcagc acaccattcg ccagcaggtt cgtcgcccag acgaagggat acaaaaccat
     2461 cagcagcggc gtatagatgt aggcggccgg cagggccagc cgctcgggat gcagagcacc
     2521 gaatgtcttg ggggcgacct cgcagaaaat cagcagcacc agcgtcagcg cgccggcacc
     2581 gaaggcaacc agaatctcgc cgcccaggcg cagcgcgatg aagccgacga gcatcggtgc
     2641 agcgacgttg acgagcgtgc tcagcagcag gatcaatccg atcaatcggt cgggatgcgc
     2701 gagcaacttc tccgccaggc gcgctgcgcg attgccctcg ttcgcactat gcctgagccg
     2761 atagcggttc acgctcatga gggcggtctc gctgcccgcg aagaacgccg cgagcagcag
     2821 cagtcccgcc aaagcgccga acaggactcc taaagtgggg tgcggcattc gtcggggcta
     2881 gccccaatga cgcccgagca ggtcctcgag gacgaatttc gcgccgaaat acgcgagcgc
     2941 gagcacgccg aagccgctca aggtccagcc ggtggcggaa cgtccgcgcc agccgaagcg
     3001 cacgcggccg atgaggagca cgccgaagat cgtccacgcg atcagcgaca acagggtttt
     3061 ctgcgtcaaa ttttgcgtaa ataagttggt gacgaacacg aagccggtga ccagcgccag
     3121 ggtgagcatg atgaagccgg tgccgatcag acggaacatc accttttcga gcacatccag
     3181 cggcggaatg gcgctcggca ggttggcgag gcgccgcgtg cgcaagcgcc gatcgagaaa
     3241 caccagcagc aaggcggtca ccgcggcggc gaacagcagc gccgccgcgc ccatcgcgag
     3301 caggatatgc gccgtcaact cccaacccgg aactgcggcc tcgacgtagc cgtgcccggc
     3361 gtcggtggcg gcggcgccga acgccgcgct cgcaaacagc agggcgccga gcacgcgatt
     3421 ttgccgctcg atcgatatga agcaggccaa gacgcccaac gtccaagcga gcagggagat
     3481 cgcctcgagc aggccgatgg caaacgagcc gttcacccgg atcatgtgca cgatggcatc
     3541 gctgtgcgcg acgatcgagg cgaggctcaa cgcccatgcg atcggctcga gccgcggctt
     3601 gttcacgctg cgcagcatga gccaggcact ggctgcatag agcaggaata aagtgatggc
     3661 gagaatcaca gctggcctgg aggatggagc gggcccaaat gacgacgccg tcgataatga
     3721 cacaagcccc agggagcgcc tagccgccgg gaaaatcgac ctgtaatatc cgcgccatgt
     3781 tcgaccgact cacgaccagt ctcgccgcgg cgctcaaatc gctgtccggc cgcggccggc
     3841 tcaccgatga gaacatctcg gagacggtgc gtgaagtgcg cctcgcgctg ctcgacgccg
     3901 atgtcgcact cggcgtcgtc aagacattca cggatgccgt caaggcccgg gcggtcggcg
     3961 tcgaagtgac caagagtctg acccccggtc aggccttcat caagatcgtg catgaggaac
     4021 tgacccggct catgggggag ccgagcaccg gcctcaatct gcgtgcgcag cgcccggtcg
     4081 tggtcctgct cgccggatta cagggcgctg ggaaaaccac cagcgccggc aagctcgcgc
     4141 gctggctgat cgaaaagcaa aacaagaaag tgctcatggt cagcaccgac gtgtatcggc
     4201 ccgcggccat cctgcagcta caaacactgg cggcgcaggt cggcgccggc tttgcgcccg
     4261 cgctcgcgag cgaagcgccg gtcgccatcg ccaagcgagc cctggtcgag gcgacgttgc
     4321 aggcctacga cgtgctgctg ctcgataccg cgggccgact gcacatagat gcggaaatga
     4381 tgacggaggt ccgtgagctc gaggcggcct tgagcccgca cgaacggctg ttcgtcgtcg
     4441 acagcatggc cggacaggac gcagtcaacg cggccaaggc attcaacgat gccttgagtc
     4501 taaccggcgt gatcttgacc aaagcggacg gcgatgcacg cggcggtgcc gcgctgtccg
     4561 tgaggcaggt gaccggcaag ccgatcttgt tcgtcggcac tggcgagaaa tccgaagcgt
     4621 tggagctgtt tcaacccgag cgcattgcgt cgcgcatctt gggaatgggc gacgtgttgt
     4681 cgctggttga acaggtccaa ggacaggtcg accaagagaa ggcggaaaaa ctcgccaaga
     4741 agatcagcaa gggaaaatca ttcgatctgt cggacttgcg cgaccagttg agccagctgc
     4801 agaacatggg cggcatgtcg gcgctattgg ataaattacc ggcacagctg caggcgaacg
     4861 cggcggcggc ggcgggtgcg gtcaacccga aaaccctgaa gcggcagatg ggcatcatcg
     4921 attcgatgac gccgcgcgag cggcggcgcc cggagctgat cgatggctcg cgcaagcggc
     4981 gcatcgcggc cggtgccggg gtgcagctgc cggaggtcaa ccggctgttg aaacagttcg
     5041 accacatgca aaagaccatg aaaaagctcg ccaaaggcgg catgttcaag aacatgatgc
     5101 gcggcatggc ggggcgccta ccgcccggat tcgggcccta gggccgctgc cggccgctcc
     5161 gtggccgacc ctttgcccgc gattcaacga gttttgttgc ccaaggaccc cgattttcat
     5221 tacaattaac ggctacgctt tgcaaggtga cccaagatgg tgacgattcg tttgactcgg
     5281 cgcggcggca agaagacccc gttctatcac gtggtggtaa ccgatagccg caagcgtcag
     5341 ggcggcacca gccttgagag cgtcgggctt ttcaatccga tcgcgcgcgg cgccgaagtc
     5401 aagctgcgcc tggacgtcgc tcgaatcgag cattggctgt cgaagggcgc gcacatgtcg
     5461 gagcgcgtca agtatcttct caccagcttt cgcaagcagg ccgcgtaagg cttaagtgag
     5521 ggggccatga gcgacgaatc atcggcgctc atccaactgg ggttcgtcgg ggcgccgttt
     5581 ggcgtccgag gttggatcaa gctacgctcg cataccgatc caccggagcg tctgctcgac
     5641 catcgacagt tgactattgg ccaggcagat gcctggcgga gctaccgaat tgaagccagc
     5701 ggccgcagcg gcggtgcgtt gaccgtgaaa ttggccggag tcgaggaccg cgaccaggcg
     5761 caggccctgc ggggcgcgca ggtgtgtgtt gcgcgcagcg aattgccgcc acgcgatgcc
     5821 cgggatttct atcgcgccga cttgatcggc tgcgaggtgg tgaatctcga tggcaaggac
     5881 ttaggatcgg tgcgccactt cgtggaaaca ccggcgcaag tgctgatggt cgtgcgcggg
     5941 acccaggaat actggattcc ggccgttccg caacatttgc gacgcgtcga tttagaggca
     6001 cggcgggtgg tcgtggactg ggacgcgtgc gcattgccgt cgtgacgttg tttccggcga
     6061 tgattcgcga ggcactgacg cacggcgtgg tcgggcgggc gctcgagcgc ggcgtgcttg
     6121 aggtcgagtg tttcgatccg cgcgaccatg cgaccgatgt gcatcgcacg gtcgatgatc
     6181 gctcgtacgg cggcggtccg ggtatggtgc tgaaggtcga accgctgcgc agcgccttgc
     6241 gtgctgcgct cgcggctgtg ccgcacggca gccggcgggt gtacctcggc gccgacggcg
     6301 tcaggttcga tcaggcgctg gcgcgcgcgg cgtgcgacgg cgcgggtttg gtgttggtgg
     6361 cgggccgcta tgagggtgtg gatgagcgct tcatcgagcg cgaaaccgac gagcagtggt
     6421 cgatcggcga ctacgtactg tcgggcggcg agctgccggc gctggtggtt attgatgcaa
     6481 ttgcgcggct gattccaggt acgctgggca gtgcggaatc ggcgcaacag gaatcgttca
     6541 gcgacgggct ggtggattgg ccgcattaca c
//
LOCUS       DASWZT010000049         9319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_53700, whole genome shotgun sequence.
ACCESSION   DASWZT010000049 DASWZT010000000
VERSION     DASWZT010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9319
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53700"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VGE92_01280"
     CDS             <1..455
                     /locus_tag="VGE92_01280"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY1312473.1"
                     /translation="SVRRTNWALLGITLVMSAAVGLKAFLLIQLTVSAFAGAAGLWLF
                     YVQHQFAGAYWARSDEWDYTAAALKGSSFYKLPRVLQWFTGNIGFHHIHHLSPRIPNY
                     HLQRCHDADPFFQTIKPVTLWGSLGALGFRLWDEQRNAFVGFSGLTRS"
     gene            complement(457..1140)
                     /locus_tag="VGE92_01285"
     CDS             complement(457..1140)
                     /locus_tag="VGE92_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY1312474.1"
                     /translation="MAIGVRTVLERNLLFRGLAPATLDQIARLCIRRSYEPDAIIFSQ
                     SDPGDALFGVVTGRVRISASTAGGREVFLNIMEPGDTFGEIALLDGRPRTASASAMAA
                     SELFLVNREQFLGLLARDPVLTDHVLRLLCARLRWVSGFAEESALLSVPARLARRLLS
                     LGKLHGHEIDSGIELKVSQDEMARFLGLSRQIVNQHLQEWKAQRWVGLGRGKIMILNG
                     RALEEVASR"
     gene            1265..1639
                     /locus_tag="VGE92_01290"
     CDS             1265..1639
                     /locus_tag="VGE92_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HEY1312475.1"
                     /translation="MKTVNYSKNIALATTAAVCLASLGMTAHADESDFRVPARMVHFS
                     DLNLNTQAGAEVLYKRIRTAAEAVCGDVDSRRLDEAAAAKACVGQAVAASIRAVNSPR
                     LTNTYNVHVGVVPATIRIASLR"
     gene            1744..3405
                     /locus_tag="VGE92_01295"
     CDS             1744..3405
                     /locus_tag="VGE92_01295"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF042664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY1312476.1"
                     /translation="MSIDWVEDEQSALSSAWHLQRTGNFVAALDLAQDSLRRWPHSQA
                     LEYLWILALASCGSTQAALDALRASSLSLNIDEDCLALEARLLKDLAFSGRADSATQL
                     IEAAAAYERVAERTGGTYSRQNAALLWMLAGDHERAIMLAAAVAKQLTLSDVPKDEES
                     AYFYWATLAEAALVLGDHAAFGRALAGANPLCRPNSWARSRTLLQMQRIAGVRPEYAA
                     LIEQWYRPPVGLVLIADWMPARLGNAVSLEAADMPALAYCFGRAPASDWHELAALGVQ
                     LHAIYPDTPLDEPTALPASLRPERDASGHLDTHTWSSLFLDETDDQQRVCAQTALGLS
                     LGQADQLRAPWVILARSQSRWCMYKDADRTALRSAFDRLDMSQEQRPKYAFLFADAVS
                     YSSLNAAETRRYWTSLLPQTAAAVLKRHAADIVVRKTWGDSVHAVFRSASAAASAALE
                     IQAATARLTEELGRGRRLIFRMALHYGPADQGMDPVEETQSVFGPQLSFAARIEPVAP
                     PGGIFVTEPFAARLSLEGAQNVHCSYVGTTSLAKSYGRVRLLRLA"
     gene            3644..4960
                     /locus_tag="VGE92_01300"
     CDS             3644..4960
                     /locus_tag="VGE92_01300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY1312477.1"
                     /translation="MEQPTSPDGTESPRRRARLLWTALVLLIVVAAVAVWWWRSRNPP
                     LHYVTARVTQGDIQRSVNMTGALNPVVTAQVESFVSGNIKSWSCDYNTLVTAGQICAL
                     IDPLPFQVIVDQDTASLHSAMAQLAKDRVAAANQAVIYAYDQKLIDQGIVSRETINAD
                     KATLDQDQSQVKLDEANIAQQRALLHGAEVNLGYTKIPSPVNGMVITRYIDVGQTVVS
                     SLSASPLFLIGKDMHDMQVDTNVSEADVGGVRPKQRAYFTVQSYPNRTFWGSVRQIRE
                     GPITVQNVVTYDVVIDVKNEDLALFPGMTADAHIITAERSNVLRVPLPATRFNPEGIA
                     RSPRAGGSARTGDSGARSGDRGGRSGGPGARAQIWLLRDGKLVSVRVRTGLDDGTLIE
                     VAGDDLKVGDVVVVNAIRPNAPPSEAQGDNRPPGANANIRGAGFRP"
     gene            4957..7071
                     /locus_tag="VGE92_01305"
     CDS             4957..7071
                     /locus_tag="VGE92_01305"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1312478.1"
                     /translation="MSDSAPGEPILCVREATKVYHLGDTEVHALRGVTLTINRGEFVA
                     IMGASGSGKSTLMNILGCLDRPSSGEYLLEGIDVAQLDEPALARIRGRRIGFVFQTFN
                     LLPRTSALENVEMPLFYAAQLEGNVTRARDMLHMLGLADRERNQPNQLSGGQQQRVAI
                     ARALVNDPAIVLADEPTGNLDSQNALDIMATIRALNLERGVTVVLVTHERELAELADR
                     IITLRDGRIISDLPTVHIAGRATRTLPAVQPYRERRFSSRIDSLLNEAGSIGWIAILA
                     ALRAIGRNKLRAGLTMLGMFIGVAALIAMVAVGEGARAAVEAQVQSLGTDLLVVLPGT
                     TRVNGVRAGNGSAASLRVKDVGGILEEDSAVADVSYVNRQGTQAVSGNQNWSTSVQGI
                     SASYLSIRRWPVVAGRTLTEQDDHDGASVCLLGQTVLTNLFGEFADPIGATVLVKNVP
                     MTVVGVLSAKGHSASGQDQDDVIFIPFRTSQERILGVATPTSAQTLSNNVFATIGPPP
                     NPFGVVPKLEGFVNTIYVQARSPALVKTALDQVTKTLEKTHRILPGKPDDFVVRDLTE
                     IAEVAEQSSKAMELLLAAIASISLVVGGIGIMNILLVSVTERTREIGIRMATGARRLH
                     VLAQFLIEAMLLALMGGMAGIAAGVVASLAISHFAGWPIRLELDVMILAFAFSGAVGV
                     FFGFHPARQASRLNPIDALRYE"
     gene            7076..8548
                     /locus_tag="VGE92_01310"
     CDS             7076..8548
                     /locus_tag="VGE92_01310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY1312479.1"
                     /translation="MRGRSPPSGIVMSVSAAWALAGCAVGPNFHQPAPPGTPGYLHPS
                     SDTTPVQAQAQDMQNVSLGNELAGEWWQLFHSAQLNEIVRTAIAASPTLVAANATLAQ
                     AREQVTVARGAFLPSVSATANAQRASAGDAVRAPGTGATANLYSIGLSTSYSPDIFGG
                     TRRAVEQQQALADYQRNQLAAAYLTLTGSVVNEVLTIASTRLQIATAEDLIASDRKNL
                     ALTQREFDVGIVPRSDVLTADSQLAADLTQLPSLHKQLDQAYDDLAVLAGRAPSEWQV
                     QPVDIDQFTLPREIPVSLPARLVRQRPDVLAAETQLHAASAAIGVAVAQEFPNISLSA
                     SITRQALQAADLFHQFDTLWGAAGSVTQPLFKGGALRAQVRAQRDAFKAEAATYQAVV
                     LEALGQVADDLWALQYDAEILTVDRHSMDVALQALKLQQQSYSVGTTTVLNLIAAERA
                     YAQARLSYVSARVQQFTDSASLLTALGGGWWNDPIEAAAR"
     gene            8636..>9319
                     /locus_tag="VGE92_01315"
     CDS             8636..>9319
                     /locus_tag="VGE92_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEY1312480.1"
                     /translation="MDESKLAQLRANFGNREVFHDGQDPVLTAAGRQVQSPEGRRSQP
                     YAGIPTFLDLPSADTAAGLDIALVGVPMDLGVSNRPGARFGPRAVRSIERIGPYHPTF
                     RGVPKAAVRAADIGDVPFRSRFSLEQSLEDIEEYYAALTAQGVRPLSVGGDHSITYPI
                     LKALGKKAPVGLIHIDAHCDTMGAFDGSKFHHGGPFRLAVLDGVLDPERTIQIGIRGS
                     SNMFWEFSHA"
BASE COUNT         1682 a         2951 c         2976 g         1710 t
ORIGIN      
        1 agtcggtgcg gcgcacgaac tgggcattgc tcgggatcac actggtcatg agcgccgcag
       61 tcggtctgaa ggcgttcttg ttgatccagc tcaccgtgtc ggcattcgcg ggtgccgcag
      121 gactctggct gttctacgtt caacatcaat tcgcgggcgc ttattgggcg cggtcagatg
      181 agtgggatta caccgcagcc gcgctcaagg gcagctcgtt ctataaattg ccgagagttc
      241 tgcagtggtt taccggcaat atcggattcc accatatcca tcacttgagc ccgcgcattc
      301 cgaattacca tcttcagcga tgtcacgatg cggacccgtt ctttcagacc atcaagccgg
      361 tcaccttgtg ggggagcctc ggagcgctcg gttttcgttt gtgggacgaa cagcgcaacg
      421 catttgtggg attttccggt ctcacccgca gctgagctag cgactcgcca cctcttccag
      481 tgcgcggcca ttcaagatca tgattttgcc gcggcccagg cccacccagc gctgcgcctt
      541 ccattcttgc aggtgttggt tgacgatctg gcgcgacagt cccaggaagc gcgccatttc
      601 atcctgcgag actttgagct cgatgccgct gtcgatctca tgaccgtgca atttaccgag
      661 cgacaacagg cgccgcgcca gccgcgcagg caccgatagc aaggcggact cctcggcgaa
      721 gccgctcacc cagcgcaggc gcgcgcacag cagccgcagc acatgatcgg tgagcaccgg
      781 atcgcgcgcc aacaatccta ggaactgctc gcggttcacg aggaacagtt ccgaggcggc
      841 catggcgctc gcgctcgcgg tgcgcggccg gccatcgagc aaggcgatct cgccgaaggt
      901 atcgccgggc tccatgatgt tgagaaacac ttcccggccg ccggcggtac tcgcgctgat
      961 gcgcactctg ccggtgacca cgccgaacaa ggcatcgccg gggtccgact gggaaaagat
     1021 gatggcatcg ggctcgtagc tgcgccggat gcagagccgc gcaatctgat cgagcgttgc
     1081 gggtgccagc ccacgaaaca gcagatttcg ctccaacaca gtccgtacgc caatcgccaa
     1141 aatctaccct ccggttgccg gccgccaatt gtaagtcttg tgtcaacgcg ttgacagaac
     1201 tcgggggccg atgcgctcaa catgactctc gaagacaaac caaacacgct caaaggagaa
     1261 agtcatgaaa accgtcaatt acagcaagaa catcgcattg gccaccaccg ccgccgtgtg
     1321 tctggcgtcc ctcgggatga ccgcgcatgc cgatgaatcg gacttccgag tgcccgctcg
     1381 catggttcac ttctcggacc tgaacttgaa cacgcaagcc ggcgccgaag tgctctataa
     1441 acggatccga actgccgcag aagcagtgtg cggtgacgtg gattctcggc gcttggacga
     1501 agcggcggcg gccaaggcct gtgtcggcca ggcggttgcc gccagcatcc gcgcggtgaa
     1561 cagcccgcgg ctgaccaaca cctacaacgt gcacgttggt gtggtaccgg ccaccatccg
     1621 catcgcctcc ctgcgctgat gcttcgggac cggagccgca tgggcccgag aggctcatgc
     1681 ggtttccgct gcggtgagca gcgcggcgtt gtaggctgca tgctgcggcg aggagcgctc
     1741 caagtgtcca tcgattgggt tgaagacgaa cagtctgcgt tgagctcggc ctggcatcta
     1801 cagcgcaccg gcaattttgt ggcggctctc gatctggcgc aggatagctt gcggcgctgg
     1861 ccccattctc aggcgctcga atatctgtgg attttggcct tggcctcctg cggctcgacc
     1921 caggcggcgc tcgatgcatt gcgtgcgagt tcgctgagct tgaatatcga tgaggattgc
     1981 ctggccctcg aggcgcgcct gctcaaagat ttggccttca gcggccgcgc cgattccgct
     2041 acccaactga tagaggcggc cgcggcatac gaacgcgtcg cggaacgcac cggcggcacc
     2101 tacagccgtc aaaacgccgc cctgctgtgg atgctcgcgg gtgatcacga gcgtgcaatc
     2161 atgctggccg ctgcggtcgc caagcaattg accctctccg atgtgccgaa ggatgaggag
     2221 agcgcttact tttattgggc gactctggcc gaggccgcgc tggtgctcgg cgatcacgcg
     2281 gccttcggtc gtgcgctcgc cggcgcaaat cccttatgcc gccccaactc ctgggcacgc
     2341 tcgaggacat tgctgcagat gcagcggatc gcgggtgtgc gccccgaata cgcggcactg
     2401 atcgagcagt ggtatcggcc cccggttggc ttggtcttga tcgcggactg gatgccggcc
     2461 cgattgggaa acgccgtgag cctcgaagcg gcggatatgc cagcgcttgc gtactgtttt
     2521 ggccgcgcac ccgcgagcga ctggcatgaa ctcgcggcct tgggcgtgca actgcacgca
     2581 atctatccgg acacaccgct cgatgagcct acggcgctgc ctgcgagtct caggcctgag
     2641 cgagatgcgt ccgggcacct cgacacgcat acctggtcga gtctattttt ggacgagacc
     2701 gatgatcagc agcgcgtttg cgcacagacg gcgctcggac tgtccttggg ccaggccgac
     2761 cagctgcgcg cgccctgggt cattctcgca agatcgcaaa gccgctggtg catgtacaag
     2821 gacgcggatc ggacggcgct gcgctcggcc ttcgatcggc tcgacatgtc gcaggagcag
     2881 cgcccgaaat atgcttttct gtttgcggat gccgtgagct actcctcgtt gaacgccgct
     2941 gaaacgcggc gttactggac gagtctcttg cctcagactg ccgcggcggt actcaagcgg
     3001 catgccgcgg acatcgtggt gcgcaaaacc tggggcgatt cggtgcacgc ggtgtttcgc
     3061 agcgccagcg cggcggcgag cgccgccttg gagatccagg ccgccaccgc acggctcacg
     3121 gaagaattag gccgcggccg ccgtctcatt tttcgcatgg cgctgcatta cggccccgca
     3181 gatcagggca tggatccggt ggaagaaacg cagtccgtgt ttggaccgca gctgtcattt
     3241 gccgcgcgca tcgaaccggt cgcgccgccg ggcggcattt tcgtgaccga acctttcgct
     3301 gcgcgcttga gcctcgaagg cgctcagaat gttcactgct cgtatgtggg caccacgagt
     3361 cttgcgaaaa gctacggacg ggtccgcttg ctgcgactcg cgtaatcgcg tcccggcgcg
     3421 agtagtcaac agcttcatcg tcaagaaacc tcgatacatc gtcaagaaat ctcgatgcgc
     3481 agttaagaaa catccgcgcg ccatcggcag cgcgatggca gtgctcgatg cgtgatgaca
     3541 gtgcttgcga atcgcggcct gcgaagtaga gtagtcccgg aacaagcgct tcacgcacac
     3601 cgtctaccga acgccgtcaa cttaagtttc gccagcgcgc agcatggaac aaccgacatc
     3661 gcccgatggc acagaatcgc cgcgccgccg agcgcggctg ctctggaccg cactggtgct
     3721 gctcatcgtg gttgccgcag tggccgtatg gtggtggcgt agccgcaatc cgccgctgca
     3781 ctacgtcacc gcgcgggtca ctcagggcga cattcagcgc tcggtgaaca tgaccggcgc
     3841 actcaatccg gtcgtgacgg cgcaggtcga gtcctttgtc tcgggaaaca tcaaatcgtg
     3901 gtcgtgtgac tacaacacgc tggtcacggc cggccagatt tgcgcgctca tcgacccgct
     3961 gccgtttcag gtcatcgtcg atcaggacac ggcgagcttg cattcggcga tggcgcaact
     4021 cgcgaaggat cgggtcgccg ccgccaatca ggccgtgatc tacgcctatg accagaagct
     4081 gatcgaccag gggatcgtgt cgcgcgagac catcaacgcc gacaaagcca cgttggatca
     4141 ggatcaatcg caggtgaaac tcgacgaggc gaacatcgcg cagcaacgcg ccttattgca
     4201 tggcgccgag gtcaatcttg gctacacaaa aattccttcg cccgtcaacg gcatggtgat
     4261 cacgcgctac atcgatgtcg ggcagaccgt ggtatcgagc ctgtcggcct cgccgctgtt
     4321 tctcatcggc aaagacatgc atgacatgca ggtcgacacc aatgtgagcg aagcggacgt
     4381 cggcggcgtt agacctaagc aacgcgccta cttcaccgtg cagagctatc cgaatcgaac
     4441 cttttggggg tcggtgcgcc agattcgcga gggccccatc acggtgcaaa acgtcgtgac
     4501 ctatgatgtc gtgatcgacg tcaagaatga ggatctcgcg ctgtttcccg gcatgaccgc
     4561 ggatgcgcac atcatcacgg ccgagcgcag caacgtgctg cgggtccctt tgcccgcgac
     4621 tcgtttcaat cccgagggaa tcgcccggtc cccgcgggcc ggcgggagcg ctcgaacggg
     4681 cgacagcggc gctcgatccg gcgacagagg cgggcgatcc ggcggccccg gcgcgcgcgc
     4741 ccagatctgg ttgctgcgtg acggcaagct ggtctcggtt cgcgtgcgca cgggtctcga
     4801 tgatggaacc ttgattgagg tcgcgggcga tgacttgaag gtgggcgatg tggttgtcgt
     4861 caatgcgatc aggcccaacg caccgccgag cgaagcgcag ggcgacaacc ggccgcccgg
     4921 cgcgaatgcc aatatccgcg gcgcaggctt caggccgtga gcgattcggc gcctggcgaa
     4981 ccgattctgt gcgtgcgcga ggcgaccaag gtctatcacc tcggcgacac ggaggtgcat
     5041 gcgttgcgcg gcgtcacgct gacgatcaat cgcggcgaat ttgtcgcgat catgggcgcc
     5101 tcgggatccg gcaagtccac gctgatgaac attctcggct gcttggaccg cccgagcagc
     5161 ggcgagtact tgctcgaggg catcgacgtc gcgcagctcg atgagcccgc attggctcgc
     5221 attcgcgggc ggcgcatcgg cttcgtattc cagaccttca atctgttgcc acggacgagt
     5281 gcgctcgaaa atgtcgaaat gccgttgttc tacgcggcgc agctcgaggg caacgtcaca
     5341 cgcgcacgcg acatgctgca catgttgggg ctggccgatc gcgagcgcaa tcagcccaat
     5401 cagctctccg gtggacagca gcagcgcgtc gccatcgcgc gcgcgttggt caacgatcca
     5461 gccatcgtgc tcgcggatga gcctaccggc aatctcgatt cgcagaatgc gctcgatatc
     5521 atggcgacca tccgtgcctt gaatctcgag cgcggcgtga ccgtggtgct ggtgacgcac
     5581 gagcgtgagc tggcggaact ggcggatcgg atcatcacgc tgcgcgatgg gcggattatt
     5641 tccgatttgc ccaccgtgca catcgccggc agagccacgc gcactctgcc ggccgtgcaa
     5701 ccgtatcgtg agcgtcgctt ctcgagccgc atcgacagcc tgctcaatga ggccggctcg
     5761 atcggctgga ttgcaatcct cgccgcgctt cgcgccattg gcaggaacaa actgcgcgcc
     5821 ggcttgacga tgctcggcat gttcatcggc gtcgccgcac tgatcgcgat ggtcgccgtg
     5881 ggcgagggcg cgcgtgccgc cgtcgaggcc caggttcaga gcttgggcac ggatctgttg
     5941 gtggtgctgc cgggcaccac gcgcgtgaac ggggtgcgcg ccggtaacgg cagcgccgcg
     6001 agtttgcgag tcaaggacgt cggcgggatt ctcgaagagg acagtgctgt ggccgatgtc
     6061 agctacgtca atcgccaggg cacgcaggcg gtcagcggca atcagaactg gagcaccagc
     6121 gttcagggca tatccgcgag ctatctttcg attcgacgct ggccggtggt ggcgggtcgc
     6181 acgctcaccg agcaggacga tcacgacggc gcgagcgtgt gcctcttggg tcagaccgtg
     6241 ctcacgaacc tcttcggcga attcgccgat ccgatcggcg ccaccgtgtt ggtcaaaaac
     6301 gtgccgatga ccgtggtcgg cgtgttgagt gccaagggcc attcggccag cggccaggat
     6361 caggatgacg tgatattcat cccgttcagg acctcgcagg aacgcattct gggtgtcgcc
     6421 acgcccacct ccgcgcagac cctgtcgaac aatgtgttcg cgaccatcgg gccgcctccc
     6481 aatcccttcg gcgtcgtgcc gaagctcgaa ggattcgtca acacgatcta cgtccaggcc
     6541 agaagcccgg cgttggtgaa gacggcgctc gatcaggtga ccaagacgct cgagaaaaca
     6601 caccgcatac tgccgggcaa acccgatgat ttcgtggtgc gcgatctgac cgagatcgcc
     6661 gaagtggcag aacagagttc gaaagccatg gaactcttgc tggcggccat cgcctcgatc
     6721 tcgctggtgg tcggcggcat cggcatcatg aatatcctgc tcgtatccgt gacggaacgg
     6781 acgcgcgaga tcggcattcg catggccacc ggcgcgcggc ggctgcatgt actggcgcag
     6841 tttctcatcg aggcgatgct gctggcgctg atgggtggca tggcgggcat cgccgcgggc
     6901 gtggtggcat cgctcgcgat ctcgcacttt gcgggttggc cgatacggct cgaactcgac
     6961 gtgatgatcc tggcgtttgc cttctcgggg gcggtgggcg tcttctttgg cttccatccg
     7021 gcccggcagg catcgcgcct gaatccgatc gatgcgctgc gctacgagta agcgcatgcg
     7081 cggccgatcc ccgccgtccg ggatcgtcat gagcgttagc gccgcttggg cgctcgccgg
     7141 ctgcgcggtc ggtcccaact ttcatcaacc cgcccctccg ggcacacccg gctacctgca
     7201 cccgagctcc gatacgacac cggtgcaggc tcaggcgcaa gacatgcaga atgtgtcgct
     7261 cggtaacgag ctcgccggtg agtggtggca actgtttcac tccgcgcaat tgaacgaaat
     7321 cgttcgaacc gcaattgccg cgagccccac gctcgtggcc gcgaacgcga ccctggcgca
     7381 ggcccgcgag caggtgacgg tggcgcgggg cgcgtttctg ccgagcgtta gcgcgaccgc
     7441 caatgcacag cgagcgagcg cgggtgatgc ggttcgagcg ccgggcacgg gcgcgaccgc
     7501 taatctctac agcatcggtc tgtcgaccag ctacagcccg gatatattcg gcggcacacg
     7561 gcgcgcggtc gagcagcagc aggcgctcgc ggactaccag cgcaatcagc tggccgcggc
     7621 ctacctcacg ctcaccggca gtgtcgtgaa cgaagtgctg accattgcgt caacacgatt
     7681 gcagatcgcg accgcggagg acctgattgc gagcgatcgc aagaacctgg cgctgacgca
     7741 gcgcgaattc gacgtgggta tcgtgccgcg cagcgacgtg ctcacggccg acagccagct
     7801 ggctgccgac ttgacccagt tgccatccct gcacaagcaa ctcgatcagg cgtatgacga
     7861 cttggcggtg ctcgccggcc gcgcaccctc cgagtggcag gtgcagcccg tcgacatcga
     7921 tcaattcacc ttaccgcgag aaattccggt gagcctgccc gcccggctgg tgcgccagcg
     7981 tccggatgtt ctcgccgccg aaacacaact tcacgcggca agcgcggcga tcggcgtcgc
     8041 cgtcgcccag gaatttccga acatcagctt gtccgcgtcg atcacacgac aggcgctgca
     8101 ggcggccgat ctgtttcatc agttcgacac gctctggggg gctgccggct cggtgacgca
     8161 gccgctcttc aagggcggcg cgttgcgtgc gcaagtgcgc gcgcagcgcg atgccttcaa
     8221 ggccgaggcc gccacctatc aggccgtggt gctcgaggcg ctcgggcagg tcgccgacga
     8281 tctgtgggcg ctgcagtacg atgcagagat cctcaccgtc gatcgacatt cgatggacgt
     8341 cgcgctgcag gccttgaagc tgcagcagca aagctactcg gtgggtacga ccacggtgct
     8401 gaatttgatc gcggcggaac gcgcctacgc gcaggcgcgc ctcagctacg tgagcgctcg
     8461 cgtccagcag ttcaccgata gcgccagcct gttgaccgcg ttgggcggcg ggtggtggaa
     8521 cgatccgatt gaggcggctg cacgctaagc gccgaccgcg tctcgtccga ccaatttgtg
     8581 tcctacaatg gctgggtaca acgaaattgc cggtgggagc agacggggaa ccactatgga
     8641 tgagtcgaag cttgcgcagt tgcgcgccaa ttttggcaat cgggaggtat ttcacgacgg
     8701 acaggacccg gtcctgacgg cggccggccg ccaggtgcaa tcgcccgaag gccggcgctc
     8761 ccagccttac gccggcatcc cgacgttcct cgacctgcct tcggccgaca ctgcggcggg
     8821 gctcgacatc gcgctcgtcg gcgtgccgat ggacctcggc gtctcgaatc gccccggcgc
     8881 acgcttcggg ccgcgcgccg ttcgcagcat cgagcgtatc ggaccctatc acccgacgtt
     8941 tcgcggcgtg cccaaggcgg cggtgcgggc ggctgatatc ggcgatgttc cgtttcgcag
     9001 ccgcttcagc ctggaacaat cgctcgagga catcgaggag tactacgctg cactcacggc
     9061 gcagggcgtg cggccgctgt cggtcggcgg cgaccattca attacttatc cgattctcaa
     9121 ggcgttggga aagaaggcgc ccgtgggcct gatccacatc gacgcgcatt gcgacaccat
     9181 gggcgccttt gacggctcca aattccatca cggcggcccg tttcgactcg cggtactgga
     9241 cggagtgttg gaccccgagc gcacgattca gatcggcatt cgcggctcgt ccaatatgtt
     9301 ctgggagttc tcgcatgcc
//
LOCUS       DASWZT010000050         4235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_10187, whole genome shotgun sequence.
ACCESSION   DASWZT010000050 DASWZT010000000
VERSION     DASWZT010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10187"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VGE92_01320"
     CDS             <1..192
                     /locus_tag="VGE92_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312481.1"
                     /translation="SGNADTGAEPFPAGTFVNGGVQNVGSMSSNTWNESCWTFSYLNN
                     SIPPAGTFTGVVRYTLSAP"
     gene            189..674
                     /locus_tag="VGE92_01325"
     CDS             189..674
                     /locus_tag="VGE92_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312482.1"
                     /translation="MNAQRRGTPLSAFFLACAFSIIWGPQAHAKTIQIDDSGTQSLEP
                     SVSLHWKSVVPSRSGTDNLMLGTSTIRVRLNVMPWLHRSLRIYLNLPAQQPGPLAISW
                     LTQGRFIPGQLRSGNRVLLYAGPITAPFLEDTLNVQYSVDGSLVRRPFPVTFHFETDE
                     D"
     gene            677..1465
                     /locus_tag="VGE92_01330"
     CDS             677..1465
                     /locus_tag="VGE92_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312483.1"
                     /translation="MRGLVFSLSLLAAAAAPAVHAQGFAALVSPPRFELVSQSGKTVR
                     SVIEVSNRSTAPAHYLVHTADWTFSPDFSVNFKDALQADSCRPWVAIERPDIVVPGGG
                     TMRYRFEVNVPADAPAGECRFAIMIEGADPSIARTQGLDMPVTGRIGVIVYLTVGDGA
                     PELEVLGPKVAVLNGQQIPILRVHNAGTAHGRVTGFLTGTDAKGVAYDFTPSDFPILP
                     HEEREVFLTPSTPKDDHPTLTFPVTVKGTLEWGKHKTELNQQFR"
     gene            1462..3480
                     /locus_tag="VGE92_01335"
     CDS             1462..3480
                     /locus_tag="VGE92_01335"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HEY1312484.1"
                     /translation="MKRRANLAVQLAFGLCGLAGLACSGRADAQVAAAAPPAAPEYQD
                     RYIGGGSLKPDISTGDNATSDTDGLARSLQIDAVASVLSSRGGGSTDNLTEDGVVVKS
                     QWETATYGAWSLDAALRTGSSDQGPAEQGQGGVITLRQRGMPFDGDWQADNGLGDLNS
                     PDINLARIQQRFYLPTAPMQGLTTEWRGPSELQIVAGGGVPGLYDGIVVPNFRTLDGS
                     TATAGAEWSPASHWTVGGQLIEAHDVNLAIGPVIDGPALMSSTTGLMTAAFADQNQHL
                     QFNLLDGDVNGKANGIGTWIDGSMVDGRFLQNAGVFRIDPNLTWGNQLISNDMQGGYY
                     RLNYQSRQWLADVGVDEVHSVSGLGTNTTFLTGDSRYQLTRDWGIGGVANASRTDGGT
                     SWSLEGYVDQVNDLGTGRAQADYADTTEGRDATLTLNENWTTPVGTRFSTAASVERIS
                     GALYNGLAQSSTILSLAAYGGGQFTTALGLEGNVRWAAAVQGQAAPGVSANVSLTWQL
                     TPAWEILATYYDSRVGSWTPLVVESPLTPPLATPVPAAQERGIFLTLRYRRASGSHFA
                     PLGGAPGAGSGEIAGLVFLDSNNNGQSDAGEVGAANVTVVLDGRFSMQTDANGRFVFP
                     VVATGHHIITVVADNLPLPWVLVNGGRKEVEVTTRDRTEISIAAQRPR"
     gene            complement(3484..>4235)
                     /locus_tag="VGE92_01340"
     CDS             complement(3484..>4235)
                     /locus_tag="VGE92_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312485.1"
                     /translation="FELVGNETISKFVDTTAAPLSPQADSPLPEQANYYDVGVQQKIN
                     DRLTIGLDSYYKQSHNLIDEGQFGAPIILTPFNYAYGKQYGLELTNSFSASDFTAYLN
                     LAWQYAHGKDIDSAQFNFDAAELAYIAQNYIHLDHEQEITASGGVSYVWDHTRISADL
                     LVGSGLRADLTLPDGSSIPNGAHLPYYTQVNLGANHIFHLGEAGTLTTRVDLINVFDK
                     RYEIRDGTGVGVGAPQFGPRRGLFFGISKSI"
BASE COUNT          761 a         1309 c         1364 g          801 t
ORIGIN      
        1 agcggcaacg ccgataccgg agccgagccc tttccggcgg gtaccttcgt gaacggcggt
       61 gtgcagaacg tcggatccat gagcagcaac acctggaacg agagctgctg gacgttctct
      121 tatttgaaca actccattcc gccggccggc acgtttaccg gcgtggtccg ctacacgctg
      181 agtgcgccgt gaacgcgcag cgccgcggga cgccgctgtc ggcttttttt ttggcctgcg
      241 cattctccat aatctggggg ccgcaggctc acgccaagac gattcagatc gatgactcgg
      301 gcacacagtc gctcgaacct tcggtgagtt tgcattggaa gagcgtggtg ccgtcgcgtt
      361 cgggcaccga caacctgatg ctcggcacat cgacgattcg cgtgcggctc aatgtgatgc
      421 cctggctgca tcgctcgctg cgcatttatt tgaacttgcc ggcgcagcag ccggggccgc
      481 ttgcgatttc ctggctgacg caggggcgtt tcattcctgg gcagctgcgc tccggcaatc
      541 gcgtgctgct ctacgccggt ccgatcaccg cgccattctt ggaggacacg ctcaacgtgc
      601 agtacagcgt ggatggctcg ctggtacggc ggcctttccc ggtgaccttt catttcgaga
      661 cggatgagga ttgatcatgc gaggacttgt attttctctg tcgctgctcg cggctgccgc
      721 cgcgcctgcc gtgcatgcgc aaggcttcgc ggcgttggtg tcgccgccgc gcttcgaact
      781 cgtctcgcag tccggaaaaa ccgtgcgcag cgtgatcgag gtgagcaatc ggtcgaccgc
      841 gcccgctcac tatttggtgc acaccgcgga ttggactttc tcgccggact tcagcgtgaa
      901 tttcaaggat gcgctgcaag cggatagctg ccggccgtgg gtcgcgatcg agcggcccga
      961 catcgtggtg cccggcggcg ggaccatgcg ctatcgcttc gaagtcaacg tgccggcgga
     1021 tgcgccggcc ggcgagtgtc gattcgccat catgatcgaa ggcgccgacc cgtcgatcgc
     1081 gcgcacgcag ggcctggaca tgccggtgac cgggcgaatc ggcgtgatcg tgtacttgac
     1141 ggtgggcgat ggtgcgcccg aactcgaggt gctcgggccg aaggtcgcag tcttgaacgg
     1201 tcagcagata ccgattttgc gcgtccacaa tgccggcact gcgcacggcc gcgtcacggg
     1261 ctttctgacc ggcaccgatg ccaagggcgt cgcctacgat ttcacgccgt cggattttcc
     1321 catcctgccg cacgaagaac gcgaagtgtt cttgactcct tcgacgccca aggatgatca
     1381 cccgactctc accttcccgg tgacggtgaa gggcacgctc gagtggggta agcacaagac
     1441 agagctcaat cagcagttca gatgaaacgc cgcgccaacc tcgccgtcca gctcgcgttc
     1501 ggcctgtgcg ggttggccgg actcgcctgc tcggggcgcg ccgacgcgca ggttgcggcg
     1561 gccgcgccgc cggccgcacc cgagtatcaa gatcgataca tcggtggcgg ctcgctcaag
     1621 ccggatattt ccacgggcga caatgcaacc agcgacaccg atggcttggc gcgttcgctg
     1681 cagatcgatg cggtcgcgag cgtcttgagt tcgcgcggcg gcggttctac cgacaatctg
     1741 accgaggacg gcgtggtcgt gaaatcgcaa tgggaaacgg ccacgtacgg cgcttggtcg
     1801 ctcgacgcgg cgctgcgtac cggcagctcc gatcagggac cggccgagca aggccaggga
     1861 ggcgtgatca cgctgcgtca gcgtggcatg cccttcgacg gcgattggca ggcggacaat
     1921 ggcctcggcg atctgaattc tccggatatc aatttggcgc gcattcagca gcgattttac
     1981 ttgccgacgg cgccgatgca gggcctcacc accgagtggc gcggaccgtc ggagttgcaa
     2041 atcgtcgcgg gcggcggagt tcccggcctg tatgacggaa tcgtcgtgcc caatttccgc
     2101 accctcgacg gctccaccgc taccgccggc gccgagtggt cgccggcatc gcattggacg
     2161 gtcggcggcc agctcatcga ggcacacgac gtcaatctcg cgatcggccc ggtgatcgac
     2221 gggcccgcgc tgatgtcctc gaccacgggc ctgatgaccg ctgccttcgc cgatcaaaac
     2281 cagcatctgc aattcaatct cctcgacggc gacgtcaacg gcaaggccaa cggcatcggc
     2341 acctggatcg acggctcgat ggtcgatggc cggttcttgc aaaacgccgg cgtgtttcgc
     2401 atcgacccga acctcacgtg gggcaaccag ctgatctcga acgacatgca aggcggttac
     2461 taccggctca actatcagag ccggcaatgg ttggcggacg tcggtgtcga cgaagtgcat
     2521 tccgtgtcgg gcctgggcac caacacgacg tttctcaccg gcgactcgcg ctaccagctg
     2581 acgcgcgact ggggcatcgg cggcgtcgcc aatgccagcc gtaccgatgg cggcacgagc
     2641 tggtcgctcg agggctacgt ggatcaggtg aatgacttgg gtaccggccg cgcgcaggcg
     2701 gattacgccg ataccaccga aggccgcgat gccacgctca cgctgaatga aaactggacg
     2761 acgccggtgg gcacgcgctt cagcacggcg gcatcggtgg aacgcatcag cggggctctg
     2821 tacaacggac tggcgcagag cagcaccatt ttgagcctcg ccgcgtacgg cggcggacaa
     2881 ttcaccaccg cgctcggctt ggaaggcaat gtgcgttggg cagccgcggt gcaaggacag
     2941 gcggcgccgg gagtatccgc caatgtctcg ttgacctggc aactcacgcc ggcgtgggag
     3001 atcttggcga cgtactacga cagccgggtc ggctcatgga cgccgctggt ggtggaatcg
     3061 ccgctgacgc cgccgctcgc gacgccggtt ccggcggcgc aggagcgcgg tatttttctc
     3121 accttgcgct atcgtcgagc ctcgggttcg catttcgcac cgctcggcgg tgcgccgggc
     3181 gccggctccg gcgagatcgc aggcttggta tttctcgaca gcaacaacaa cggccaatcc
     3241 gacgcgggcg aggtcggcgc cgccaacgtc acggtcgtgt tggacggccg attctcgatg
     3301 cagacggacg ccaacggccg tttcgtattt ccggtggtcg ctaccggcca ccacatcatt
     3361 acggtcgtcg cggacaacct gccgctgccc tgggttctgg tcaacggcgg acgcaaggag
     3421 gtggaggtca cgacgcgcga tcgcaccgaa atcagcatcg ccgcgcaacg cccgcgctga
     3481 cggctagatc gacttcgaga taccgaaaaa caatccccgg cggggaccaa attggggagc
     3541 acctaccccg accccggttc catcgcggat ctcatagcgt ttgtcgaaca cgttgatcag
     3601 atcgactcgg gtcgtgagcg tacccgcctc gcccaggtga aagatgtggt tggcaccgag
     3661 attcacctgc gtgtagtaag gcaggtgggc gccgttcgga atggaacttc cgtcaggcaa
     3721 agtcaggtcg gctcgtagcc cggagccgac cagcaagtcg gcactgatgc gcgtgtgatc
     3781 ccagacgtag gagacgccgc ccgaagccgt gatctcctgc tcgtgatcca agtggatgta
     3841 attctgggcg atataggcta attcagccgc gtcgaaattg aactgcgccg aatcaatatc
     3901 cttgccatgc gcgtactgcc acgcgaggtt taggtacgcg gtgaaatcgc tggcggagaa
     3961 actgttggtc aattcgaggc cgtattgctt gccataggcg tagttgaacg gtgtgagaat
     4021 gatcggcgcg ccgaattgtc cctcatcgat caagttgtgc gactgcttgt agtagctatc
     4081 gagcccgatg gttaatcgat cgtttatctt ctgctgcacg ccgacatcat agtaattggc
     4141 ttgttccggc agcggagaat ccgcctgcgg tgacaacggc gccgccgtgg tatcgacgaa
     4201 tttcgagatg gtttcattgc cgacgagctc aaagg
//
LOCUS       DASWZT010000051         2785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_44037, whole genome shotgun sequence.
ACCESSION   DASWZT010000051 DASWZT010000000
VERSION     DASWZT010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44037"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VGE92_01345"
     CDS             complement(<1..533)
                     /locus_tag="VGE92_01345"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY1312486.1"
                     /translation="MPVASALLACLHPAQAAENSEAATLEEVVVTAQKRTENLQDVAV
                     SIQAISTEKLEELRIANFDDYAKFLPTVSFQSDGPGFEHVYMRGVVSGGDGNHSGSLP
                     SVGVYLDEQPVTTIDGNLDIHVYDIERVEALSGPQGTLYGASSESGTIRIISNKPDPS
                     GFKAGYDLGVNSVDHGGT"
     gene            761..943
                     /locus_tag="VGE92_01350"
     CDS             761..943
                     /locus_tag="VGE92_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HEY1312487.1"
                     /translation="MQYQGLFGLVVLIADVWAIVNVFQSTADTGKKVLWTVLIILLPV
                     LGFILWYFLGPKTGRS"
     gene            1023..1736
                     /locus_tag="VGE92_01355"
     CDS             1023..1736
                     /locus_tag="VGE92_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEY1312488.1"
                     /translation="MRPRSHEQHSTSGISWLRASVLGANDGIVSTSSLILGVAAAHVS
                     QTNILLSGTAALVAGAMSMATGEYVSVHSQADTEAAALSQERTELAADFVGERHELTE
                     IYVRRGLDAKLAAQVAAQLMAHDALGSHARDELGFTAASRPRPLQAAVVSAGSFASGA
                     ILPLLVAAWAPPQHRMVSIITVSLLCLLLLGGWSAQVGGADRIKGALRVAFWSALAMA
                     VTTLIGAIAGSLQAPGAVN"
     gene            1733..>2785
                     /locus_tag="VGE92_01360"
     CDS             1733..>2785
                     /locus_tag="VGE92_01360"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEY1312489.1"
                     /translation="MTSAARPLETALLSVAQMTEADRATIAAGTPGSVLMRNAGNAVA
                     REIIRRWTPCPVTVLCGPGNNGGDGFVAAGVLARSGWPVRLALLGQTHDLRGDARLHA
                     AEWTAAVEPVGIAAIEGAALIVDALFGTGLRRPLEPVAALTLAAAAERGMPLIAVDVP
                     SGVLGDTGASLGAAAAVCTVTFARKKPAHVLLPGRELCGEIVVADIGIPRAVLEALPI
                     DTWENDPTLWRTLLPHAGAAGNKYTRGHALLIGGYPMTGAARMAARAAARVGAGLTTI
                     AVPQVAFAIYAAALTSIMVQPLSRDGDLARLLGDPRYSACLIGPGAGVSDATRAAALE
                     ILATARPVLLDADAISV"
BASE COUNT          425 a          897 c          918 g          545 t
ORIGIN      
        1 gtgccgccgt ggtcgacgga attgacgccg aggtcgtagc ccgccttgaa gccgctcggg
       61 tccggtttgt tcgagatgat acgaatcgtt cccgattcgg aactcgcgcc gtacaaggta
      121 ccctgcggcc cggacaaggc ctcgacgcgt tcgatgtcgt agacatgaat gtccaagttg
      181 ccgtcgatgg tggtgaccgg ctgttcatcg aggtagacac ccacgctcgg cagcgagccg
      241 gaatgattgc cgtcgccgcc gctcacgacg ccgcgcatgt agacgtgttc gaagccgggc
      301 ccgtcgcttt gaaaggacac ggtgggcaga aacttcgcat agtcgtcgaa attggcgatg
      361 cgcagctctt cgagcttttc ggtgctgatc gcctgaatgc tcaccgccac gtcttgcagg
      421 ttttcggtgc gcttctgagc ggtaaccacc acctcctcga gggtcgccgc ctcgctgttc
      481 tcggcggcct gggcggggtg caggcacgcc aacagggcgg atgcgaccgg cactgccgtc
      541 cacctcgccc gggcgcggtt tctggcttga atgcgcttga gttttcgctg tcggcttcga
      601 gtcattcatc atccccttgc agtttttttc agagtgtctc cagggcaggt tatgacattt
      661 cggccgatat tccaattgac gccgcgccgc cgttgtcaac cgccccggct gaatgcacac
      721 tgttcggcgt tcgcggcgag tccgcaaagg tgtataaaaa atgcaatacc agggtttgtt
      781 cggcctcgtc gttctgattg ccgacgtctg ggcgatcgtc aatgtctttc agagtaccgc
      841 cgatacgggc aaaaaagttc tctggacggt tctcatcatc ctgcttcccg tgctcggatt
      901 cattctttgg tattttctgg gacccaagac cggcaggtcg tagcgccggc aagcgcgccg
      961 ttggaactgc gcggtgtggt tccctgctct acagatccgc tgttggtata tacaggacag
     1021 tcatgcgtcc acgcagtcac gagcagcata gtacgagcgg aatcagctgg ctgcgagcca
     1081 gcgttctcgg cgcgaatgac ggcatcgtat cgacgtccag cttgatcctg ggtgttgccg
     1141 cggcgcacgt ttcgcagacc aatatcctgc tgagcggcac ggcggcgctg gtcgccggag
     1201 caatgtcgat ggcgaccgga gaatatgttt ccgtccattc gcaagcggac accgaagccg
     1261 ccgccctgtc gcaggagcgc accgagctcg cagccgactt cgtcggcgag cgccatgaac
     1321 tgaccgagat ttacgtgcgc cgcggactcg acgccaaact tgccgcacag gttgccgcgc
     1381 agctgatggc gcacgacgcg ctcggctcgc atgcccgcga cgaattgggc ttcaccgccg
     1441 cctcgcggcc gcgtcctttg caggctgccg tcgtgtcggc cggcagtttt gcatcgggcg
     1501 ccatcctgcc gctgctggtc gcggcctggg ctccgccgca gcatcgaatg gtctccatca
     1561 taacggtgtc gctcctgtgc ttgttgcttc tcggcggctg gtccgcccag gtgggaggcg
     1621 ccgacagaat caagggtgcg ttgcgcgtgg cgttttggag cgcgctcgcc atggcggtca
     1681 ccacgctgat cggtgcgatt gccggtagtc tgcaagcgcc cggagccgtg aattgacgag
     1741 cgcggcgcgc ccgctcgaga ccgcgctgct cagcgtggcg caaatgaccg aggccgatcg
     1801 cgccaccatt gcagcgggaa cgcccggatc ggtcctcatg cgcaacgccg gcaacgccgt
     1861 cgcccgcgag atcattcgac gctggacgcc ttgcccggtc acggtgctat gcgggcctgg
     1921 aaataatggt ggtgatggct tcgtcgccgc cggcgttctt gcccgaagtg gctggcccgt
     1981 tcggcttgcc ctgctcgggc aaacgcacga cctgcgcggc gatgcgcgct tgcatgcggc
     2041 cgaatggacc gctgccgtcg agccggttgg gattgcggcc atcgagggcg ccgcgctgat
     2101 cgtagacgcc ttgttcggca ctggtctgcg ccgcccgctc gagcccgtgg ccgcgttgac
     2161 gctcgccgcc gccgcagagc gcgggatgcc gttgatcgcg gtcgatgtgc cgagcggagt
     2221 cctcggcgac accggcgcca gccttggcgc cgcagcggcc gtgtgcaccg tgacgttcgc
     2281 acgcaagaaa cctgcccatg tgctgttgcc cggccgggag ctgtgcggcg agattgtggt
     2341 agccgatatc ggcatcccgc gcgcggtgct cgaggcgctg ccgatcgaca cttgggagaa
     2401 cgatccaacg ctgtggcgca ctctgctgcc gcatgccggt gccgcgggca acaagtacac
     2461 gcgcggacac gcattgctca tcggcggcta cccgatgacc ggcgctgcac ggatggccgc
     2521 gcgcgccgcg gcgcgcgtcg gcgccggtct cacgaccatc gccgtgccgc aagtcgcgtt
     2581 tgcgatttac gctgcggctc tgaccagcat catggtgcag ccgctgagtc gcgacggcga
     2641 cctggcacgg ctgttgggcg atccgcgcta tagcgcctgt ctcatcgggc cgggcgccgg
     2701 cgtcagtgac gcgactcgcg ccgccgcact cgagatcctg gcgacggcac ggccggtgct
     2761 gctcgatgcg gatgcgatca gcgtg
//
LOCUS       DASWZT010000052         3405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_24676, whole genome shotgun sequence.
ACCESSION   DASWZT010000052 DASWZT010000000
VERSION     DASWZT010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24676"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VGE92_01365"
     CDS             <1..454
                     /locus_tag="VGE92_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312490.1"
                     /translation="LISDVGKYNDKGVGNPFKRSCGSGCHNTLLMGMDLAPGYMDLGL
                     NNYPWATPRPDLPLFKVVCDPSAPAQSYLGRVIYTHDPGRALITGKCIDVGASMTQQS
                     RGLAARAPYFMNGSAANLRELVDFYDRRFNIGYTEGEKQDLVNFLSVL"
     gene            499..1305
                     /locus_tag="VGE92_01370"
     CDS             499..1305
                     /locus_tag="VGE92_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312491.1"
                     /translation="MIALAGCAALASEPPGTPVSFVACPIARDTGPDTDLCFIADHAD
                     KRYALVNPADWGVPQLKHRVLVEGRVTQGSFCGAATIEGRASVLPDIDDSCNALLPFD
                     GVIKGMAGGVFNSGSPQQRAAAEDLARRAALDPALSVQPVILDPPASPPPQPPFDARS
                     LTITYPFDSDRGPGPDMVKLKDLAAYANAAHAKRVDVVGYRAESRLMDGGELTERASL
                     AEARARKIAGIVGALGIAPEKIHLRWESTAIPGKGDLDWLNRKVEVTVTP"
     gene            1318..2358
                     /locus_tag="VGE92_01375"
     CDS             1318..2358
                     /locus_tag="VGE92_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312492.1"
                     /translation="MRPRIEWLAFGYFATYVPYAVLVRWLATVQYPPLGRPLTGLEIL
                     PATTILSGVLTLVWIGASGWWRFAHQTRLFGISLVRPTRWTFLSGVGTAMLLFTVPLS
                     FTFQGVSIPFMQLLMRGDVLVIAPLVDLIARRRVHWYSWVALLLVAMGLVLTIGARGG
                     LHLPPLAIVTVVLYTLGYFVRLAVMTKIGKSGDTSIVKGYFVEEKMVAIPVAVLVLAL
                     LSALGWGAQGNQLSFGFVGVWASNQLPPIFGLSLLLLLVSLFSILILLDPRENTFCVP
                     IERSASILAGTAAAYILAGMSLGKPPTAAEACGAALLTAAIIVLSLGPRIRHAATLGA
                     PWQRPSGNGPRR"
     gene            2322..3383
                     /locus_tag="VGE92_01380"
     CDS             2322..3383
                     /locus_tag="VGE92_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY1312493.1"
                     /translation="MAKTLRKRTKTLRKKPAARAVPLRLASTTIDDVAAAARVSTATV
                     SRFFNAPALLSKTTAERVRAAVERTGYVPNMMAGGLASSRSRLVAAVIPTISQSIFSS
                     TIQALTDSLAEVGYTVMLGLTGAADEHVHRQLLSIIGRRPDGVILTGSLLDAAARRQL
                     KATKINTIETWDLPASPIDLVVGLSHQKVGVAVARRALSQNRRCAFVVSANGVRALAR
                     RNGFSLTMLQGGAPEPTTATFSGTTTFGQGRAAIAAHLDGGGRPEIVVCTSDWSAHGV
                     LDELQRRGVRVPDDIAVIGFGDLDFAADLNPALTTVKIDGGIIGRQAAKFLMQRARGE
                     KIDNPIVDIGFSLIVRQSG"
BASE COUNT          566 a         1068 c         1091 g          680 t
ORIGIN      
        1 cctcatcagc gatgtcggca agtacaacga caaaggcgtc ggcaatccct tcaaacgctc
       61 gtgcggcagc ggctgtcaca atacgctgtt gatgggaatg gacctcgctc caggctatat
      121 ggatctgggg ctcaacaatt atccctgggc caccccgcgg ccggatttgc cgctgttcaa
      181 agtcgtttgt gatccgtcgg ctcctgcgca gtcctatctg gggcgcgtca tctatacaca
      241 tgaccccggg cgggcgctga tcaccggaaa atgcatcgac gtcggcgcca gcatgacgca
      301 gcagtcacgc ggtctcgccg cgcgggcgcc gtatttcatg aacggatcgg cggccaactt
      361 gcgggagctc gtggactttt atgatcggcg cttcaacatc ggctacaccg agggcgaaaa
      421 gcaggacttg gtgaacttcc tgagcgtcct atgaggcacg caggtgaatt gattcggctc
      481 cggcgcgcaa gctgcgcaat gattgcgttg gcgggttgcg cggcgctggc ctcggagccg
      541 cccggcaccc cggtgtcgtt cgtcgcctgc ccgatcgcgc gcgacacggg ccccgacacc
      601 gacttgtgct tcatcgccga tcatgcggat aaacgctatg cgctcgtcaa tccggccgac
      661 tggggcgtac cgcaactcaa acaccgggtg ttggtggagg gccgcgtcac gcagggaagt
      721 ttttgcggcg ccgccaccat cgaaggccgc gcatccgtgc tgccggacat cgatgactca
      781 tgcaacgctc tgctgccgtt cgatggcgtc atcaaaggca tggccggcgg tgtgttcaac
      841 agcggctccc cgcagcagcg cgccgccgcc gaggatctgg cgcgccgcgc cgcgctggat
      901 cctgctcttt ccgtgcaacc cgtgatcctc gatccaccgg cctcgccgcc gccacagcct
      961 ccattcgacg cgcgctcgct cacgatcact tatccgttcg atagcgatcg gggcccggga
     1021 cccgacatgg tcaagctgaa agaccttgcg gcgtatgcga atgccgcgca cgcgaaacgc
     1081 gtggacgtcg tgggttaccg ggctgagtcg cggttgatgg atggcggcga actcacggag
     1141 agggcatcgt tggccgaagc gcgggcccga aaaatcgcgg gcatcgtggg cgctctcggg
     1201 attgcgccag agaaaattca cctccgctgg gaaagcaccg ccattcccgg caagggcgac
     1261 cttgattggc tgaaccgcaa ggtcgaagtc acggtcactc cctgatgcga gactgaattg
     1321 cgacctcgca tcgaatggct cgcgtttggg tatttcgcga cctatgttcc ctatgccgtg
     1381 ttggtgcgct ggctggcaac ggtgcagtac ccgccgctcg gccggcccct gacgggtctg
     1441 gaaatcctgc cggcgaccac gatcttgagc ggcgttttga ccctggtgtg gataggggcg
     1501 agcggctggt ggcgtttcgc acatcagacc cggcttttcg gcatttcact ggtgcgaccg
     1561 acgcgctgga cgtttctctc tggcgtgggc accgccatgc tcctgttcac cgtgcctctg
     1621 tcattcacct ttcagggcgt aagcatcccc ttcatgcaac tgctgatgcg cggcgacgtg
     1681 ttggtgattg cgccattggt ggatttgatc gcccgccgac gcgtgcattg gtattcgtgg
     1741 gttgcattgc tattggttgc catgggactc gtcctcacga tcggcgctcg cggcggcttg
     1801 catctgccgc cgctcgccat cgtcacggtc gtgctctata cgctgggcta tttcgtgcgg
     1861 cttgccgtga tgacgaagat cgggaaatcc ggcgatacga gcatcgtcaa agggtacttc
     1921 gtggaggaga aaatggtcgc catcccggtg gccgtgctgg ttctcgcact cctgtccgct
     1981 ctgggttggg gtgcacaggg caatcagttg agcttcggct tcgtcggtgt gtgggcgagc
     2041 aaccaattgc cgccgatctt cggactctcc ctgctattgc tgctggtgtc gctgttttcg
     2101 attttgatcc tgctggatcc ccgcgagaat actttttgtg tgccgatcga acgatcggcg
     2161 agcatcctcg ccggcaccgc ggcggcttac atcctcgcgg gaatgtcgct cggcaagccg
     2221 ccgaccgccg ccgaagcgtg cggcgcagcg ctgctgaccg cagccatcat cgttttgtcg
     2281 ctcggccccc ggatccggca cgctgctaca ctgggggcgc catggcaaag accctccgga
     2341 aacggaccaa gacgttaaga aaaaaaccgg ccgcgcgcgc cgttcctctg cggttggcga
     2401 gcacgaccat cgatgatgtg gcagccgcgg ctcgagtgtc gacggccacc gtttcgcgtt
     2461 ttttcaatgc accggcgttg ctctccaaaa ccacggccga acgggtccgc gctgcggtgg
     2521 agcgcaccgg gtacgtgccg aacatgatgg cgggcggttt ggcctcgagt cgttcacgcc
     2581 tggtagcggc ggtcataccg accatctcac agtcgatttt ttcgagcacc atccaggccc
     2641 tgacggattc gctggccgaa gtcggctata ccgtcatgct cggcttgaca ggtgcggcgg
     2701 acgaacacgt gcaccgtcaa ctgctgtcga tcatcgggcg ccgtcccgac ggcgtgatcc
     2761 tgaccggatc tctgctcgat gcagcggcgc ggcggcagct caaggccacg aaaatcaata
     2821 cgatcgagac atgggatctg ccggcgtcac cgattgattt ggtggttggg ttgtcgcatc
     2881 agaaggtggg agtcgccgtc gcgcgtcgcg cgctgtcgca gaatcgccgc tgcgcattcg
     2941 tggtgtcggc caacggcgtg cgcgccctag cgcggcgcaa tgggttttcg ctcaccatgc
     3001 tgcaaggtgg ggcgccggaa cccacgacgg ccacattcag cggcaccacc accttcggcc
     3061 aggggcgcgc cgcgattgcc gcgcacctcg acggcggcgg ccggccggag atcgtggtgt
     3121 gcacttccga ctggagcgcc cacggggtgc tcgacgagtt gcagcgccgc ggtgtccgcg
     3181 taccggacga catcgcggtc attggcttcg gtgacctgga ttttgcggcg gatctcaatc
     3241 cggcgctgac caccgtgaaa attgacggcg gcatcatcgg acgccaggcc gccaagttcc
     3301 tgatgcagcg ggcacgcggc gaaaaaatcg acaaccccat cgtggacatc ggtttttctc
     3361 tgatcgtgcg gcagagcggc tgacggccgg tgcccggcgt caaag
//
LOCUS       DASWZT010000053         9933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_29492, whole genome shotgun sequence.
ACCESSION   DASWZT010000053 DASWZT010000000
VERSION     DASWZT010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9933
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29492"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VGE92_01385"
     CDS             <1..615
                     /locus_tag="VGE92_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011532463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HEY1312494.1"
                     /translation="QVKWTADASARRVSLEGLTRAQIAGWKPGERLLLSGKLLTGRDA
                     AHKRICALLDADKPLPDGLDFHGRVIYYVGPVDPVGTEVVGPAGPTTANRMDRFTDTM
                     LGKAGLLAMVGKAERGPETVAAIARHASAYLIAVGGAALLVSNAIRSSRVVAFAELGM
                     EAVYEFDVRDMPVTVAVSSEGISLHDTGPKAWAGKFAGIPVSVA"
     gene            complement(627..1274)
                     /locus_tag="VGE92_01390"
     CDS             complement(627..1274)
                     /locus_tag="VGE92_01390"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEY1312495.1"
                     /translation="MSTYTSTQLRPVRAALWLCMAFGAGIAGWSTTAVGAILKIGQPA
                     PDFIAVDSKGDSLQLSQYRGKTVVLEWTNAECPYTRKHYSSGNMQSIQMLAQKNGVVW
                     LTVVSSAPGKQGFVTGAQADALTHSRNAAPTAVLLDPQGTVGRLYNAKTTPHMFVIDK
                     NGALQYMGGIDSIATADVSDIPNAEPYLKEAMLAVQQGNPVAHPVTQPYGCSIKY"
     gene            1480..3372
                     /gene="mnmG"
                     /locus_tag="VGE92_01395"
     CDS             1480..3372
                     /gene="mnmG"
                     /locus_tag="VGE92_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004654475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEY1312496.1"
                     /translation="MRFATEYGVIVIGGGHAGAEAALAAARTGQRTLLLTQNIETLGQ
                     MSCNPAIGGIGKGHLVKEIDALGGAMALAADQAGIQFRTLNSSKGAAVRATRVQADRQ
                     LYKRAIRRMLETQTNLTLFQQEAADLIVEGERVVGVVTQSGIQLRARAIVLTVGTFLG
                     GRIHVGLESHAGGRAGDPPSLKLAARLRELNLRVGRLKTGTPPRIDGRSIDFSVMREQ
                     HSDDPRPVFSYLGARHLHPRQMPCHITATNERTHDIIRAASARSPMFTGLIAGVGPRY
                     CPSVEDKVVRFADKHSHQIFVEPEGLDSHEIYPNGISTSLPFDVQLEFVRSIAGFENA
                     HITRPGYAIEYDFFDPRDLKSSLESKLLGGLFFAGQINGTTGYEEAAAQGLLAGVNAA
                     RLADGHDAWCPKRSEAYLGVLVDDLITRGAPEPYRMFTSRAEYRLSLREDNADLRLTA
                     LGRELGLVDDTRWSFFQRKGLAVAGELARLAATRVRPADVDAGLLAKLGAPLLRETHA
                     LDLLRRPQLEYDDLQQVAADAAAWRDDESLAEQVKLQVDVQAKYSGYLKRQHEEIERQ
                     RRHEELRLPRDIDYAEVGGLSNEVRECLREVRPETLGQAARIPGLTPAALSLLLVHLK
                     KRDCAA"
     gene            3401..3880
                     /locus_tag="VGE92_01400"
     CDS             3401..3880
                     /locus_tag="VGE92_01400"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004278946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEY1312497.1"
                     /translation="MTSFYDFAVQGLDGSQDILTPLRGKVTLAVNVASKCGFTPQYAG
                     LEELYQELRDQNFSVVGFPCNQFGAQEPGSAAEIQSFCSLTYGVSFKLAEKIDVNGPH
                     RHPVYAWLTAKDNGFPGDIGWNFEKFLIARDGRLVCRYPSATKPTDPTFLQDLADVL"
     gene            3987..4613
                     /locus_tag="VGE92_01405"
     CDS             3987..4613
                     /locus_tag="VGE92_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY1312498.1"
                     /translation="MLSRLNGMAWLATALSLSWSLGAYAAGNPAEGEKKFYTCFGCHG
                     IENYRNAYPDYSVPKLRHQNASYIVAALQEYRSGERPHPTMHAQAASLSDQDMEDIAA
                     YLQGAEPVKANAAVNGKVPKQVSACVACHGENGLGVDAPLTPKPAVIAGQHVDYLEQA
                     LTAYRNGRRKNVVMDGMARLLTSDEDVKIAAAYFAGQTSPLVSATTKN"
     gene            complement(4632..6575)
                     /locus_tag="VGE92_01410"
     CDS             complement(4632..6575)
                     /locus_tag="VGE92_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY1312499.1"
                     /translation="MMTRFHCLLLLAASLACGAAAGALPRIERGALLLDNIPETAPAL
                     SEKLNAYLSARQATPLAFSPRGQLLISTRFGDVEQLHLVEHAGGERRQLTFLREPITQ
                     AAFSPDPGRSAVVYLKDTSGNEKAQLYYQRVGEPAAKLLTDGKSLNGSPVWSNAGREV
                     AFCSTARDAVSYDVNIVEPETGALPRLAVSGDGSAWYPLDWSPDDRKLLVLKHVSAAE
                     SYLYVVDLGTGQKREVEPAPGKVAITAAKFSRDGQGVYLISDRDSEFTQLRYVNLFTA
                     EKTVVSGRLSWDIEDLAIARDGHYLAYISNEAGIDKLNLLDLRTHQDLIPPRLPAGGI
                     VSSLSFDADGKRLAFGFAAPNRPRDAYVLDVAANQLEAWTHSEPGAVDLAKFITPRLT
                     QFPTFDRVDGRERQIPLYVYEPATPGVHPVLVLLHGGPESQFRPGFDPWLQFVVSELG
                     FAVVAPNVRGSSGYGKSYLALDNGVLREDAVKDVGALLVWLSLQNSFDPKHVLVAGDS
                     YGGYLTLAALVNFSERLRGGVEMAGIGDFVGFETDAAPDRQNQRRAEFGDERDPDMRA
                     FLRRISPLTNTERIVRPLLIVHGKNDPWVSVSQSEQLLNRIRSRGGEVWYLQAGDEGH
                     GFAKLANREAYYRAFAQFLVTNK"
     gene            6603..6992
                     /locus_tag="VGE92_01415"
     CDS             6603..6992
                     /locus_tag="VGE92_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312500.1"
                     /translation="MEAMATYEHLLKSYDVRDQLDEISSADPPVYLRRCLAEGFSAAN
                     LNFARIQQLAVCVMVVDAVVNGRDYELFEHELIADWRIHYAGACAKLKDSGVQVLHRI
                     LEYDRPQDPDAVAEIEQLASSVAASNG"
     gene            6985..7629
                     /locus_tag="VGE92_01420"
     CDS             6985..7629
                     /locus_tag="VGE92_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312501.1"
                     /translation="MDERRQGLRRQAQWMALGAALLSMLAACRTAAPPPQAAAEPAQS
                     QAPTDSSYDWHGLLIAPFGSALKDIPAQLHEVLLFHDAAHGATADEDAECYAADTSAP
                     RFVGRTPDEYLLCFKRDRLARVQASVRLAEAQASEAFATACALWLKNAAAPAGQPDAP
                     APGAPATNSASDSAACEGSQGDIRFSGRLQQPGPAESPQDEIALTITLDSVANP"
     gene            7635..8270
                     /gene="rsmG"
                     /locus_tag="VGE92_01425"
     CDS             7635..8270
                     /gene="rsmG"
                     /locus_tag="VGE92_01425"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEY1312502.1"
                     /translation="MTEASEHELLTAGAHELGIELAAGSAEALKRLIDELEIGNAQFN
                     LTGIRDRPGMLRKHVLDSLSVQPYLRGSRVADIGTGAGFPGLPLAIVNPQRRFTLVEA
                     TGKKARFVEQTARRLGCDNVQVEHARAENYRPFELFDTVVARALSSLADFAAYASHLC
                     APQGRLLAMKGKRPDDEISALPKSFRVLAVHRLKVPGLEDERHLVEFSSKS"
     gene            8267..9052
                     /locus_tag="VGE92_01430"
     CDS             8267..9052
                     /locus_tag="VGE92_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014705464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY1312503.1"
                     /translation="MKRVIAVANQKGGVGKTTTAVNLAASLSATKRRVLLIDLDPQGN
                     ATMGCGIEKHAQARTSCDVMLGDCTAMEALVPVEYGGFMLMPANQDLTAAEVRLLGMA
                     SGREFRLRNALKPVREDFDVILVDCPPSLNMLTLNALVAADSVMVPMQCEYYALEGLS
                     SLMQTIEQVRSGINPELEIDGLLRTMFDPRNNLSNEVSAQLIEHFGDKVFRTVIPRNV
                     RLAEAPSFGKPVLFHDKDSRGALAYLALAGEMIRRDDLPSAPV"
     gene            9091..>9933
                     /locus_tag="VGE92_01435"
     CDS             9091..>9933
                     /locus_tag="VGE92_01435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY1312504.1"
                     /translation="MSGKKPSLGRGLAELSPLLAKRAVAAETEQAPATPGVLAGERLA
                     NLPLDLLERGKYQPRSDMRTESLSELADSIKSQGLVQPILVRPIGGRDPGESQRYEII
                     AGERRWRAAQMAGLAQIPAVIREVPDEAALSMALIENIQREDLNPLEEAGALQRLVDE
                     FGLTHQSAAEAVGRSRAAVSNLLRLLELADEVKQLLEQRRIEMGHARALLGLGSRRQQ
                     IEVAALVAKKSLSVRDTEALVRRIISPQSGAVKDASQPDPDIQRLERDLADKLGAKVA
                     FQHHV"
BASE COUNT         1842 a         3119 c         3213 g         1759 t
ORIGIN      
        1 caagtcaaat ggaccgccga tgcaagcgca cgacgtgtat cgctcgaggg cttgacccgc
       61 gcgcaaatcg ccggctggaa acccggcgaa cgcttgctgc tgtcagggaa actattgacc
      121 ggccgcgatg ccgctcacaa gcgcatttgc gccctgctcg acgcggacaa gccgctgccc
      181 gacggcttgg attttcatgg ccgcgtcatc tattacgtgg gccccgtgga tccggtcggc
      241 acggaagtcg tcgggccggc cggtcccacc accgccaatc gcatggaccg ctttaccgac
      301 accatgctcg gcaaggcggg actgctcgcg atggtgggca aggcggagcg cggcccggaa
      361 acagtcgccg ccatcgcccg gcacgcgtcc gcctacttga ttgccgtggg cggcgcggcc
      421 ttgctggtct cgaatgccat tcgcagcagc cgcgtagtcg cctttgccga actcggcatg
      481 gaggccgtgt acgaattcga cgtgcgcgac atgccggtga cggtggcggt gagttcggaa
      541 ggcatttcgc tgcacgacac cggtcccaag gcctgggccg gcaagtttgc cggcatcccg
      601 gtgtcggtgg cgtgagccgg tgacggctag tacttgatgg agcagccgta aggttgcgtc
      661 accgggtgcg ccaccggatt gccctgttgg accgccaaca tcgcctcttt gagatacggc
      721 tccgcgttcg ggatatcgct tacgtccgcg gttgcaatgc tatcgatacc gcccatgtac
      781 tgcagcgcgc cgttcttgtc gatcacgaac atatgcggcg tggtcttcgc attgtagagg
      841 cgacccaccg tcccctgagg atccagcagc accgccgtcg gcgcggcgtt gcgcgagtgc
      901 gtcagcgcat ctgcctgcgc gccagtgacg aagccctgct tgcccggtgc cgaggacacc
      961 accgtcagcc acacgacgcc atttttttgc gccagcatct gaatgctttg catgttgccg
     1021 ctcgaatagt gcttgcgcgt gtaagggcac tcggcgttgg tccactccaa gaccacggtc
     1081 ttgccgcggt actggctcag ctgcagcgaa tcgcccttgc tgtcgacggc gataaaatcg
     1141 ggcgcgggtt ggccgatctt aaggatggcg cccacggcag tggttgacca gccggcgatg
     1201 cccgcgccga acgccatgca cagccacagt gcggcccgca ccgggcgcaa ctgggtcgat
     1261 gtataagtgc tcatttcgca tctcctgtcg aaaacctgag tccggtcccc ggggtcgagc
     1321 catgggtgga tgaggtcaga aatcccttag ttgccgccgg ggccgcgtag gttccccacc
     1381 gcttgggggt tccctggcgc tttggcgata ccgcgcatag agtagccgcc ttgcagaaaa
     1441 tcgagtggat tcagcgcgat gagtttcggt gcgaaagtga tgcgttttgc tacggaatac
     1501 ggcgtaatcg tcatcggcgg cggccatgcc ggcgccgaag ccgcactggc ggctgcccgg
     1561 accgggcagc gaactttgct gctcacgcaa aatatcgaga cgctgggcca gatgagctgc
     1621 aatccggcga tcggcggcat cggcaagggg cacttggtca aggagatcga tgccctggga
     1681 ggcgcgatgg cgctcgccgc cgatcaggcg ggaatccagt ttcgtaccct gaactcgagc
     1741 aagggagcgg cggtgcgcgc gacgcgagtg caggccgatc ggcaactcta caaacgtgcg
     1801 atccgacgca tgctcgagac ccagacgaat ctgacgctgt tccagcaaga agcggccgat
     1861 ctgatagtcg agggagagcg tgtggtgggc gttgtcaccc aaagcggcat tcagctgcgg
     1921 gcgcgcgcca tcgttttgac cgtcggcact ttcctcggcg gccggattca tgtgggattg
     1981 gaaagccatg cgggcggacg cgccggcgat cccccgtcgc tcaaactggc ggcgcgcctg
     2041 cgtgaactga accttagagt gggccgcttg aaaaccggta ccccgccgcg catcgatggc
     2101 cgcagtatcg atttttccgt gatgcgcgaa cagcacagcg atgatccgcg gccggtgttt
     2161 tcctatctcg gcgcgcgcca tctgcacccg cgccagatgc cttgccacat cacggccacc
     2221 aacgagcgta cccacgacat cattcgcgcc gcgagcgcgc gctcgcccat gttcaccggc
     2281 ttgatcgccg gtgtcgggcc gcgctactgc ccctcggtcg aagacaaggt ggtgcgcttc
     2341 gcggacaagc attcgcatca gattttcgtg gagcccgagg gactcgacag ccacgagatc
     2401 tacccgaacg gcatttccac cagcctgcct ttcgacgtgc agctcgagtt cgtgcgcagc
     2461 atcgccggat tcgagaatgc gcacatcacc cgacccggct acgccatcga atatgacttc
     2521 ttcgaccccc gcgatctgaa atccagcctg gagagcaagc ttttgggcgg cttgtttttt
     2581 gcgggccaaa tcaacggcac cacgggctac gaggaggcgg cggcgcaggg tctgctggcc
     2641 ggcgtcaatg ccgcgcgcct ggcggacggg cacgatgcgt ggtgcccgaa gcgcagcgaa
     2701 gcgtacttgg gcgtgctggt cgatgatctc attacgcgcg gggcgcctga gccctaccgc
     2761 atgttcacca gccgagccga ataccggttg agcttgcgcg aggacaacgc ggacctgcga
     2821 ctcaccgcgc taggtcgcga actcggcctg gtcgacgaca cccgttggtc gttcttccag
     2881 cgcaaaggcc tcgcggtcgc cggcgagctc gcgcgcctcg ccgcaacgcg ggtgcgcccg
     2941 gccgacgtcg atgccgggtt gctcgcgaaa ctcggcgcgc cgctgctgcg tgaaacgcat
     3001 gccttggatc tgttacgccg cccgcagctc gagtacgacg atttgcagca ggtcgcggcg
     3061 gacgctgccg cgtggcgcga cgatgagagc ctggccgagc aagtcaagct gcaggtggat
     3121 gtgcaagcga aatactcggg gtacctgaag cgccagcacg aagaaatcga gcgtcagcgg
     3181 cgtcatgaag aattgcggct gccgcgagat atcgattatg ccgaggtcgg cggactgtcg
     3241 aacgaagtgc gcgaatgctt gcgcgaggta cggcccgaaa ccctgggtca ggccgcacgc
     3301 atcccgggcc tcaccccggc cgcgctgtcg ctgctgctgg tgcatctgaa gaaacgcgac
     3361 tgcgccgctt gaggcgccgg cgcatgccga gggcaaaccg atgacgagct tttacgactt
     3421 tgcggttcag ggtctcgacg gcagccagga tattctgact ccgctgcgcg gcaaggtgac
     3481 cctggcggtg aacgtcgcga gcaagtgcgg cttcacgccg caatatgcgg ggctggaaga
     3541 actttaccag gagttgcggg accagaattt cagcgtggtc ggattcccct gcaatcagtt
     3601 tggcgcgcag gagccgggaa gcgcggcgga aattcaaagc ttttgctcgc tcacctacgg
     3661 cgtgagcttc aagctagcgg agaaaatcga cgtcaatggc ccgcatcgcc atcccgtgta
     3721 tgcgtggctg acggcaaaag acaacggctt tcccggcgac atcggctgga atttcgagaa
     3781 attcctgatc gcgcgcgatg gtcggctggt ttgccgctat ccatcggcca caaagcccac
     3841 tgaccccacg tttttgcagg acttggcgga cgttttatag cgtgcgcgat gctgccattc
     3901 agcggccgca tgcgagcaag tggcaatttg ggcgcccact ctttaacatc agcgctctat
     3961 ttgcaccctg tggcgcggag aattagatgc tgtctcgatt gaatggcatg gcgtggctgg
     4021 caacggcttt gagcttgagt tggtcgctcg gcgcgtatgc cgcaggcaat cccgcggaag
     4081 gcgagaagaa gttctacacc tgtttcggat gccatggcat cgagaactac agaaatgcct
     4141 atccggatta cagcgtgccg aagctgcgcc accagaacgc atcctatatc gtcgcggcac
     4201 tgcaagagta ccgcagcggc gagcggccgc atccgaccat gcacgcgcaa gcggcgtcct
     4261 tgtccgatca agacatggaa gatatcgccg cctatctgca aggcgccgaa cccgtgaagg
     4321 caaacgccgc cgtcaacggt aaggtcccga aacaggtgtc cgcctgcgtc gcctgtcacg
     4381 gcgagaacgg tctcggcgtc gatgccccgc tcacgccgaa gccggccgtg atcgccggtc
     4441 aacatgtgga ttacctggaa caggcactga cagcctatcg caacggccgg cgcaagaacg
     4501 tggtgatgga cggcatggcg cggcttctga ccagcgatga agacgtcaag atcgccgcgg
     4561 catatttcgc cggccaaaca tcgccgttgg tttcggcaac gaccaagaat tagacgcgcg
     4621 gcgccgggcg cctatttgtt cgtgaccagg aactgcgcga acgcccggta gtaggcctcg
     4681 cgattcgcga gcttggcgaa gccgtgaccc tcgtcgccgg cctgcaagta ccacacctcg
     4741 ccgcccctgc tgcggatccg attgagcaac tgctccgatt ggctgacgga gacccacgga
     4801 tcgttcttgc catgcacgat gagcagcggc cgcacgatgc gttcggtgtt cgtgagcggc
     4861 gatattcgcc gcagaaacgc gcgcatgtcg gggtcgcgct cgtcgccgaa ttccgcgcgg
     4921 cgttgattct ggcgatccgg cgcggcgtcc gtttcaaagc ccacgaagtc gccgatgcca
     4981 gccatctcga cgccgccgcg caggcgctcg ctgaaattga cgagcgcggc cagcgtcaga
     5041 tatccgccgt aggaatcccc cgccaccagc acatgcttgg ggtcaaagga attctgcagg
     5101 ctcagccaga cgagcagcgc gcccacgtcc ttcaccgcat cctcgcgcaa cacgccgttg
     5161 tcgagcgcca ggtagctctt gccgtagccg gacgagccgc gcacattcgg cgcgacgacg
     5221 gcaaatccga gttcgctcac cacgaattgc agccacggat cgaagcccgg gcggaactgc
     5281 gattcagggc cgccgtgcaa cagcaccagc accgggtgca cacccggcgt ggcgggttcg
     5341 tagacataca acgggatttg ccgctcgcgg ccgtcgaccc gatcgaaggt cggaaactgc
     5401 gtgagccgcg gcgtgatgaa cttagcgagg tcgaccgcgc ccggctcgct gtgcgtccac
     5461 gcctccaact ggttggcggc gacgtcgagc acataggcat cgcgcgggcg gttgggcgcg
     5521 gcgaaaccaa aggcaaggcg cttgccgtcc gcgtcgaaac tcaacgagct gacgatgccg
     5581 cccgcgggaa gccgcggcgg gatcaggtcc tggtgcgtgc gcaagtcgag cagattgagc
     5641 ttgtcgattc ccgcctcgtt gctgatatag gccaaataat gcccatcgcg cgcaatcgcc
     5701 agatcttcga tgtcccagga gagacggccg gagaccacgg tcttctcagc ggtaaacaga
     5761 ttgacgtaac gcaactgcgt gaactcgctg tcacgatccg agatcaaata cacaccctga
     5821 ccgtcgcgtg agaatttggc ggccgtaatc gcgactttgc ccggcgccgg ctcgacctcg
     5881 cgcttttgac ccgtgcccag atccacgacg tacaagtacg attcggcagc cgagacgtgc
     5941 ttcaagacca gcagtttgcg gtcatcgggc gaccaatcga gcggatacca ggcgctaccg
     6001 tcgccactga cggcgagacg cggcagcgcg ccggtttccg gttcgacgat attgacgtca
     6061 tagctcacag cgtcgcgagc cgtgctgcag aaggcgactt cgcggcccgc attggaccag
     6121 acgggggaac cgttcagcga cttgccgtcc gtcagcagtt tcgccgccgg ttcgccgacg
     6181 cgctgataat agagctgcgc tttctcgttg ccgctcgtgt ccttgaggta cacgacggcg
     6241 ctgcggcccg gatcgggtga gaatgccgcc tgcgtgatcg gctcgcgcag gaaggtgagt
     6301 tggcgccgct caccgcccgc atgctcgacg aggtgcagct gctcgacatc gccgaaacga
     6361 gtgctgatca aaagctgacc gcgcggcgag aaggcgagcg gcgtcgcctg gcgggcgctc
     6421 agataagcat tgagcttctc ggacaacgcc ggcgccgtct cggggatatt gtcgagaagc
     6481 agcgcgccgc gctcgatccg cggcagcgcg ccggccgcgg cgccacacgc cagcgatgcc
     6541 gccaatagca gcagacaatg gaatcgcgtc atcatttact atgttctcac acgacgggga
     6601 taatggaagc aatggcgacc tatgagcatc tgctgaaatc ctacgacgtg cgcgatcagc
     6661 tcgatgagat ttcgagcgcg gatccgccgg tttatttacg ccgttgcctg gccgagggtt
     6721 tctccgcggc caacttgaac ttcgcgagga tccagcaact cgcggtttgc gtcatggtgg
     6781 tcgatgccgt cgtcaacggc cgcgactacg agcttttcga gcatgaactc atcgccgact
     6841 ggcgaattca ctacgcgggc gcgtgtgcga aactgaagga cagcggcgtg caagtgctgc
     6901 accggatcct cgaatacgac cgcccgcaag atccggacgc tgtcgccgaa atcgaacaac
     6961 tcgcgagcag tgtggcggcg agcaatggat gaacggcggc aaggcctgcg ccgacaggcc
     7021 cagtggatgg cgctcggtgc ggcgttgctg tcgatgctgg ccgcttgccg cacagcggcc
     7081 ccgccgccgc aggcagcggc cgagccggcg caatctcagg cacccaccga cagttcctac
     7141 gactggcacg gcctgttgat cgcgccgttc ggcagtgccc tgaaagatat tccagcgcag
     7201 cttcacgagg tgctgctatt tcacgacgcg gcgcacggcg cgacagcgga cgaggacgcg
     7261 gagtgctacg ccgcggacac gagcgcgcca cggttcgtcg ggcgcacgcc cgatgagtac
     7321 ttgctgtgtt tcaagcgcga tcgactggcg cgcgttcaag cgtcggtgcg gttggccgag
     7381 gctcaggcgt ccgaagcatt cgccactgcg tgcgcccttt ggctcaagaa tgcggcagcg
     7441 cccgccggcc aacccgacgc cccagcgccc ggcgctcctg cgaccaacag cgcctcggac
     7501 agcgccgctt gcgaaggcag ccagggcgac atccgcttca gcggccggct gcaacagccc
     7561 gggccggcgg agtcgccgca ggacgaaatc gcattgacga ttaccctcga cagcgtcgcg
     7621 aacccgtgac ttaagtgact gaagcgtccg aacatgaatt actgaccgcg ggtgcgcacg
     7681 agctcggaat cgagttggct gcgggcagtg ccgaggcgct caagcggctc atcgacgaac
     7741 tcgagatcgg caacgcgcaa ttcaacctga cgggcattcg cgatcgcccg gggatgctgc
     7801 gcaaacatgt cctcgacagc ttgagtgtgc aaccttatct gcggggcagc cgcgtcgccg
     7861 atatcggcac cggcgcgggc ttcccggggc tgccgttggc catcgtcaat ccgcagcgcc
     7921 gcttcacgtt ggtcgaagcc acgggcaaaa aggcgcgctt cgtcgaacag accgcccggc
     7981 gcttggggtg cgacaatgtt caggtcgagc acgcccgcgc ggaaaactat aggccgttcg
     8041 aacttttcga tacagtggtg gcgcgggcgc tgtcgtcgtt ggcggatttc gcggcctacg
     8101 cctcgcacct gtgcgcgccg cagggacggt tgctggcgat gaagggcaaa cggccggacg
     8161 atgaaatttc ggcgctgccc aagtcgtttc gcgtgctggc ggtgcatcgg ctgaaggtgc
     8221 ccggcttgga ggacgagcgg cacctcgtgg agttctcgag caaatcatga agcgcgtcat
     8281 cgcagttgca aatcagaagg gcggcgtcgg caagaccacg accgcagtga atctggctgc
     8341 ctcgctctcc gccaccaagc ggcgcgtgct tttgatcgat ctcgatccgc aaggcaacgc
     8401 gaccatgggc tgcggaatcg agaagcatgc ccaagcgcgc accagttgcg atgtcatgct
     8461 gggggattgc accgcgatgg aggcgctggt gccggtggag tacggcggtt tcatgctgat
     8521 gcccgccaat caagacctca ccgcggccga agtccggctg ctcggcatgg cgagcggccg
     8581 agagttcaga ttgcgcaatg cgctgaaacc ggtgcgcgaa gatttcgacg tcattttggt
     8641 cgattgcccg ccgtctctga acatgctgac gctgaacgcg ctggtggcgg cggattcggt
     8701 gatggtgccg atgcagtgcg agtactacgc gctcgaagga ttgagttcct tgatgcagac
     8761 catcgagcag gttcgcagcg gcatcaatcc tgagctggaa atcgacggct tgctgcggac
     8821 catgttcgac ccgcgcaaca atctttcgaa cgaagtctct gcgcagctca tcgagcactt
     8881 cggcgacaag gtttttcgca ccgtgatacc gcgcaacgtg cgtttggccg aggcgccgag
     8941 tttcggcaag ccggtcctgt ttcacgacaa agactcgcgc ggcgcgctcg cctatctggc
     9001 actcgccggc gaaatgattc gccgcgacga tctgccgtcc gcgcccgtat aatcgattca
     9061 ccgatcccgc atcgttcact gggaagtctc atgtccggca aaaagccgtc tcttggtcgt
     9121 ggcctcgccg agctctcgcc gctgctcgcg aagcgcgccg tggcggccga gacggagcag
     9181 gcgccggcca ctcctggcgt gctcgccgga gaacgattgg cgaatctgcc gctggatctg
     9241 ttggagcgcg gcaagtacca gccgcgcagc gacatgcgaa ccgagtcctt gagcgaactg
     9301 gccgattcga tcaaatccca aggcctggtg cagccgatcc tggtacggcc catcggcggg
     9361 cgcgatcccg gcgagtcgca acgctatgaa atcattgccg gcgagcggcg ctggcgggcg
     9421 gcgcaaatgg cgggactcgc gcagattccc gcggtaattc gcgaggtgcc cgatgaggcc
     9481 gcgctttcca tggcgctcat cgagaatatt cagcgcgagg atctcaatcc cttggaagag
     9541 gcgggtgcgc tgcagcgctt ggtcgatgag ttcggattga cgcatcaatc ggcggccgaa
     9601 gcggtcggcc gttcgcgcgc cgcggtgagc aatctgctgc gcctgctcga gctcgccgac
     9661 gaggtcaagc aattactcga gcagcgccgg atcgaaatgg gccatgcgcg cgcgctcttg
     9721 ggcttgggct cgcgtcgcca gcagatcgaa gtggcggctc tcgtcgccaa gaagtcgctg
     9781 tcggtgcgcg acaccgaggc attggtgcgt cgcatcattt cgcctcagag cggcgccgtg
     9841 aaggacgcat cgcagccgga tccggatatc cagcgcctgg agcgcgattt ggccgataaa
     9901 ttgggcgcga aagtggcatt ccaacaccac gtc
//
LOCUS       DASWZT010000054         6297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_19807, whole genome shotgun sequence.
ACCESSION   DASWZT010000054 DASWZT010000000
VERSION     DASWZT010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19807"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2502)
                     /locus_tag="VGE92_01440"
     CDS             complement(<1..2502)
                     /locus_tag="VGE92_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1312505.1"
                     /translation="MSAPSPADDSTPRSRSLMNRIVISSLRQSFLVALMTVILIIAGT
                     RSLDRLPVDAYPDLAPPMVEIITQWPGRAAEEVERLITVPVEKEMNGIPRLVTARSIS
                     LYGLSDVILTFQNKTDNYFARQEVFNRLGDLSLPSGVTPSVAPMSAPSGLIYRYVLQS
                     PDRSPMELKTFEDWIVEPAYKSVPGVADDSGFGGGSMQYQVLLDPAKIAAIGLSASQV
                     ESALTANNDNSGGGFYSQGGQFYYVRGLGRLTTLEDIGNVVLAVHDGTPVLVKDVGRV
                     VIGIAPRLGEFGFQKQDDAVEGVILLRTGEKTQDVLKGVEAKTQELNQQILPKDVKVL
                     PFYDRSDLVALTTDTVQRNLLRGMVLVVIVLIFFLYNLRAGLIVAVSIPLALLFAFIC
                     LDLQNASANLLSIGAVDFGILVDGAVVMVENIYRQIVARNGTPATVRQIIIDAAAEVD
                     RPLFYAVAVIVAGFLPIYALAGPSGTLFKPMADTMVFALIGSLLVTLTLLPVLCAWFM
                     RNGVRERRNAAFDAIRSVYSRGLDLCLARPWMTTLGSALLLAVSLLLIPRIGAEFMPH
                     LDEGALWVRATMPYTISFDESAKIAPKVRDILHSFPEVTTVASELGRPDDGTDPTGFF
                     NVEFYVDLKPYSQWSGAYRTKAGLIEAINKKLEPFPGINFNYTQPAEDAVDEAETGLK
                     SALAVKVFGADLQTLQKKGKEIKQVLQQVRGIREVTLVQELGQPSLAININRAKIARY
                     GLNVADINSLIQTAIGGDVATQVVQAEKQFDLVVRLDQQYRDNPEEIGNILVATPAGQ
                     QIPLKEFADIKVASGASFIYRQDNSRYIGVQFSVEG"
     gene            complement(2499..3629)
                     /locus_tag="VGE92_01445"
     CDS             complement(2499..3629)
                     /locus_tag="VGE92_01445"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY1312506.1"
                     /translation="MLNKLAAAALVVSLTGCSPHVGTPPQGAEATGANVTLSAAQRQH
                     IRTYRVVKSTFHKTIEATGVVDFDNDQATSVLAPFSGPVSRLLVSLGAVVKQGDPLAA
                     VDSPDFAAALSAYQKALATAKTNRRLADLDKDLLEHSGVAQREAAQAETDAVNAEADR
                     DAALKGLQSLNVSAQVIADIQDGHPPARLEAIIRAPVAGTVVEKLITPGELLQAGTTP
                     CFTVANLSRVWVLAQLFGSDLAAISLGDSADVLADDGTPAVPGKVDNIAALVDPDTRS
                     VAVRVVTANPRGTLKKQMYVRVRIRADQESAGVLVPVSSILRDDENLPFVYFVKSDGS
                     FARQPVTLGNRSGDQYDISAGLKAGDEIVIDGGIFVQFIQNQ"
     gene            complement(3619..5115)
                     /locus_tag="VGE92_01450"
     CDS             complement(3619..5115)
                     /locus_tag="VGE92_01450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY1312507.1"
                     /translation="MKLVVISRVLRLAACAGLLLQGGCAVGPNFKRPAAPEVSDYTPN
                     PLAATSGAPNVPVGAPQRFAKGGDISADWWTLFHSQALSALIDESLTNNHDLKSAQAA
                     LLAAHENVLAQRGAFYPSVTGNFSATRQRQSGQIAPTLNSNAFLYNLFTPQVSISYVP
                     DVFGLNRRTVESLQAEEQEARFQMIATYTTLTANVVVTAIQESAVQMQVDVTRQLIDS
                     NANTVKILQYQFTKGYASRLDLAAQESQLAQITATLPPLLKQVAQLHDQLAVLAGRFP
                     SEASNEKFELSTLQLPEELPQSLPSALVAQRPDVLQAEANLHDASAKIGIAIANRLPN
                     IVLTANAGSSAAAIDQLFTSGTGFWGVGAAVTAPIFQGGTLLHEERAAKAAYTQAAEQ
                     YRSTVLTAFQNVADTLTALEQDAEAVKAAAAAADAAKITLDLAKRQWQDGYSGYLALL
                     AAEQADQQSRISLVQAQANRFADTAALFQALGGGWWHRADLAAEKHAQ"
     gene            complement(5248..>6297)
                     /locus_tag="VGE92_01455"
     CDS             complement(5248..>6297)
                     /locus_tag="VGE92_01455"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1312508.1"
                     /translation="MFKWVADTLVQEAGVTPLLHRLGVAPIMENGTIRGVITESKAGR
                     EAILAKRVIDATGDADIAARAGAPVRKSPKQEMMAVSVMFSMSGVNKKEFIDAVKADP
                     QTYADWSGNGEWDYETTGKEDQLFSPFLRKPFQRALEAGLIPPSLKTIAGTWGTVTDQ
                     GDLTYLNLVHVPEIDGTDPNDLTKGEMRGRREAIYALEALRGYMPGCEHAKLRNFGMT
                     LGIRDTRKIDAHYNLTGTDVREQARFDDAIGIFPEFIDGYGILILPTTGRYWHLPYRA
                     LLPKGVANLLVAGRCIGGDKISHASARNMMCCAVSGQGAGVAAAVSLKRDEALDELNI
                     AAVHQELTRQGARFR"
BASE COUNT         1192 a         1897 c         1959 g         1249 t
ORIGIN      
        1 cccctcgacc gagaactgca cgccgatgta gcgcgaattg tcttggcggt agatgaacga
       61 ggcgccgctc gcgaccttga tgtccgcgaa ttccttgagc ggtatttgct gaccggcggg
      121 agtggcgacc aagatattgc cgatttcttc cggattgtcg cggtactgct ggtcgaggcg
      181 caccaccaga tcgaattgct tctctgcctg taccacttgc gtcgcgacgt cgccgccgat
      241 ggcggtttgg atcaaggagt tgatgtcggc gacgttcaat ccgtagcggg cgatttttgc
      301 acggttgatg ttgatcgcga gactgggttg cccgagctcc tgaaccaggg ttacttcccg
      361 aattccgcgt acctgctgca agacctgttt gatctccttg cctttctttt gcagcgtctg
      421 caaatcggca ccgaacactt tgaccgccag cgcgcttttc agacccgttt ccgcctcatc
      481 cacggcgtct tccgccggct gcgtgtagtt gaaattgatg cccggaaaag gttcgagctt
      541 tttgttgatg gcctcgatca gaccggcctt ggtgcgatat gcgccgctcc attgcgagta
      601 aggcttcagg tcgacgtaga actcgacatt gaaaaagccg gtcggatcgg tgccgtcatc
      661 gggccggccg agttccgatg ccaccgtggt gacctccgga aaggaatgca gaatgtcgcg
      721 tactttgggc gcgatcttcg ccgattcgtc gaaggaaatg gtgtagggca tggtggcgcg
      781 cacccacaag gcaccctcat ccaaatgcgg catgaactcg gctccgatac gcggaatcag
      841 cagcagcgaa acggcgagca gcagggcgga tccgagcgtc gtcatccacg gacgagccag
      901 acagaggtca aggccccggc tgtaaaccga tcggatcgcg tcgaaggctg cgttgcggcg
      961 ctcgcgtacg ccattgcgca tgaaccacgc gcacagcacc ggcagcaacg tgagcgtgac
     1021 gaggagcgag ccgatgagcg cgaataccat cgtgtccgcc atcggcttga acagcgttcc
     1081 ggagggtccg gcaagagcgt agatgggcag gaagccggcg acgatcaccg ccaccgcgta
     1141 gaacaatgga cgatccactt ccgccgccgc atcgatgatg atctgccgca cggtggccgg
     1201 agtgccgttg cgtgccacga tttggcggta gatgttctcg accatgacca ccgcgccgtc
     1261 caccaggatt ccgaaatcca cggcgccgat cgacagcaga ttggcggagg cgttctgtag
     1321 gtccaggcaa atgaacgcga acaacaaagc cagcggaatg ctgacggcga cgatcaatcc
     1381 ggcgcgcaaa ttgtaaagaa aaaagatcag cacgatgacc accagcacca tgccgcgcaa
     1441 caggttgcgc tgcaccgtat ccgtggtcag agccacaaga tcgctgcggt cgtagaacgg
     1501 caaaaccttg acgtccttcg gcaagatctg ctgattgagt tcctgggtct tggcttccac
     1561 tcccttgaga acgtcctggg ttttctcgcc cgtgcgcagc aggatgacac cttcgacggc
     1621 gtcatcctgc ttttggaacc cgaactcgcc aagccgcgga gcaatgccga tgacgacgcg
     1681 gccgacatcc ttcaccaaga ccggagtgcc gtcatgcacc gcgagcacca cgttgccaat
     1741 gtcctcgaga gtcgtgaggc gcccgaggcc gcgcacgtag tagaactggc cgccctgcga
     1801 atagaacccg ccgcccgagt tgtcattgtt ggcggtgagc gcgctttcca cttgcgatgc
     1861 cgatagcccg atagcggcga tttttgcagg atcgagcagc acctgatact gcatcgagcc
     1921 gcctccgaat ccagaatcgt ccgcgacgcc ggggacggat ttgtaggctg gttcgacgat
     1981 ccagtcctcg aatgtcttca gttccatcgg cgatcgatcg gggctttgca gaacgtagcg
     2041 atagatgagc cccgacggcg ccgacatcgg cgcgaccgaa ggcgtcacgc cgctcggcaa
     2101 actgagatcg cccagccggt tgaatacctc ctggcgcgca aaataattat cggtcttatt
     2161 ttgaaaggta agaatgacgt ccgaaagccc gtacagcgag atcgagcggg cggtgaccag
     2221 ccgtgggata ccgttcatct ccttctcgac cggcaccgtg atcaggcgct ccacttcttc
     2281 tgcggcgcgg ccgggccatt gggtgatgat ctcgaccatg ggaggtgcca gatcggggta
     2341 agcatcgacc ggcagccgat ccagcgagcg ggtgcccgcg atgatgagaa tcaccgtcat
     2401 gagcgcgacc agaaacgact ggcgcaacga ggagatcacg atgcggttca tgagggagcg
     2461 gctgcgcggc gtagaatcgt cggcgggcga gggcgcgctc attgattctg tatgaactgc
     2521 acaaagatgc cgccatctat cacaatttca tcgcccgcct tgaggccggc gctgatgtcg
     2581 tattgatcgc cgctgcggtt gcccagggtc acgggctgcc gcgcaaagct gccgtcggac
     2641 ttgacaaagt agacaaatgg cagattttcg tcgtcccgca atatcgacga caccggcacc
     2701 agtacgccag cgctttcctg gtcggcgcga atccgcactc gcacgtacat ctgtttcttt
     2761 agagtgcctc gcggattggc ggttactaca cgcaccgcca ccgatcgggt gtcggggtcg
     2821 accagcgcgg cgatattgtc taccttgccg ggaacagcgg gcgtgccgtc gtcggccagt
     2881 acgtctgctg aatcgccgag actgattgcg gctaaatccg agccgaaaag ttgcgccagg
     2941 acccagacgc gcgacaggtt ggcgacggta aagcatgggg tggtccccgc ctgcagcagt
     3001 tctcctggcg tgatcagttt ttccacgacg gttcctgcaa ccggcgcacg gatgattgcc
     3061 tcgaggcggg cgggcgggtg gccgtcctga atgtccgcaa taacctgagc ggaaacgtta
     3121 agcgactgca atcctttcag ggcggcgtcc cgatccgcct cggcattgac cgcatcggtt
     3181 tccgcctgcg cggcctcgcg ctgcgcgacg ccgctgtgct cgagcagatc cttatcgaga
     3241 tcggccagcc gccgatttgt cttggcggtg gcaagcgcct tttggtacgc gctcaacgcc
     3301 gccgcgaagt cgggcgaatc gaccgccgcc agcggatcgc cttgtttgac cactgcaccg
     3361 agagaaacca ggagccgcga tacgggacct gagaaaggcg ccaacacgct ggtagcctga
     3421 tcgttgtcga aatcgacgac cccggtggct tcgatcgtct tatggaacgt ggacttcacg
     3481 acgcggtaag tgcgtatatg ctggcgttgc gcggcgctca aagtgacatt ggcgcccgtc
     3541 gcttccgcac cctgcggagg ggtgccgaca tggggcgaac aaccggtgag cgaaaccacg
     3601 agcgccgccg ccgccaattt attgagcatg tttttccgcc gctaagtccg cgcgatgcca
     3661 ccagcccccg ccgagtgctt gaaacagcgc ggccgtatcg gcaaagcgat tggcttgggc
     3721 ctgcaccaga ctgatccggc tttgttgatc cgcttgttcg gccgcgagca acgcaagata
     3781 tccgctatag ccgtcctgcc attggcgctt ggccaagtcg agcgtgatct tcgcagcatc
     3841 cgccgctgcg gccgcagcct tcaccgcttc tgcatcttgt tcgagcgccg tcaaggtatc
     3901 ggcgacattc tgaaaggcgg tcagaaccgt gctgcgatat tgttctgccg cttgggtgta
     3961 agcggccttc gcggcgcgct cttcgtgcag gagcgtgcct ccttgaaaga tcggcgcggt
     4021 cacagccgca cccacgcccc aaaacccggt gcccgacgtg aacagctgat cgatggccgc
     4081 ggccgaactg ccggcgttcg ccgtcaaaac gatgttgggc aatcgatttg cgatcgcgat
     4141 gccgatcttg gcgctggcgt cgtgcaaatt ggcttcggcc tgcaagacgt ccgggcgttg
     4201 cgctaccagc gccgacggca ggctctgcgg cagctcttcg ggcagctgca gagtcgagag
     4261 ttcgaacttc tcgttgctcg cctcgctcgg aaagcggccc gcgagaaccg ccagctggtc
     4321 gtgcagctgc gccacttgct tcagcagcgg cggcagtgtg gcggtgattt gtgcaagctg
     4381 cgattcctgc gccgccaggt cgagccggct ggcatatccc ttggtgaatt gatattgcag
     4441 gatcttcacg gtgttggcgt tgctgtcgat gagctgacga gtcacgtcaa cttgcatctg
     4501 cacggcgctt tcttggatcg cggtgacgac gacatttgcc gtcaaggtgg tgtaggtggc
     4561 gatcatttga aagcgggctt cctgttcctc ggcctgcagc gattcgacgg tgcgccggtt
     4621 cagaccgaag acgtcgggaa cgtaggaaat gctgacttgc ggcgtgaaca ggttgtagag
     4681 aaaggcattg gagttcagcg tcggggcgat ttgcccggat tggcgctggc gggtcgccga
     4741 gaagttcccg gtgacgctcg ggtagaacgc accgcgctgg gcgaggacgt tctcgtgagc
     4801 tgccagcaat gccgcctgcg cggacttgag gtcgtgattg ttggtcaggg actcgtcgat
     4861 cagagcgctg agcgcctggg aatggaacag cgtccaccag tccgccgaaa tatcgccgcc
     4921 cttggcgaag cgttgcgggg cgcccacggg cacattcgga gcaccgctcg tcgcggcaag
     4981 gggattgggt gtgtagtcgc tcacctcggg agcggctggc cgcttgaaat tgggtccgac
     5041 ggcgcagcca ccctgcagca gcagaccggc gcacgccgcg agacgcaaca cacgggaaat
     5101 aaccaccaat ttcatacgaa cagtcttagg aagcgccgcg tgatgctgat ttccacctta
     5161 tttttcggag cccgattgcg cagtcgcgat gttgatcgat catccgacag catagccgca
     5221 aaggaattcc ctgttatcgg cgacggatca cctaaagcga gccccctggc gcgtcagttc
     5281 ttggtgcaca gcagcgatgt tcagctcatc caaggcctcg tcacgcttga gcgaaaccgc
     5341 ggcggcgacg cccgcacctt ggccgctcac tgcgcagcac atcatattgc gtgccgaggc
     5401 atgcgagatt ttgtcgcccc cgatacagcg cccggcgacc agcagattgg ccaccccttt
     5461 gggcagcagc gcgcggtagg ggaggtgcca gtagcgtccc gtggtcggca gaatcagaat
     5521 gccgtaaccg tcgatgaatt ccggaaatat gccgatcgca tcgtcgaaac gggcctgttc
     5581 gcgtacatcg gtgccggtca gattgtagtg cgcgtcgatc ttgcgggtgt cgcggatacc
     5641 gagcgtcatg ccgaaattgc gcagcttggc gtgttcgcag cctggcatgt agccgcgcag
     5701 cgcttcgagg gcatagattg cctcgcgcct gccgcgcatc tcgcctttgg tcaggtcatt
     5761 cggatcggtc ccgtcgattt cgggcacgtg cacgagattc aaataggtca gatccccctg
     5821 gtccgtgacg gtaccccagg tgccggcaat ggttttcaag ctcgggggaa tgaggccggc
     5881 ctcgagcgcc cgttggaaag gcttgcgcag gaacggcgag aacagttgat cttccttgcc
     5941 cgtggtttcg taatcccatt ctccgttgcc cgaccagtcg gcgtaggtct gcgggtctgc
     6001 cttgaccgca tcgatgaatt ccttcttatt gacgccactc atcgagaaca tcacggaaac
     6061 cgccatcatc tcctgcttgg gcgacttgcg cacgggcgca ccggcgcggg ccgcgatgtc
     6121 ggcatcgccc gtggcatcga tcacgcgctt ggcaagaatt gcttcgcggc cggccttgct
     6181 ctcggtgatg acgccgcgaa tcgtgccatt ctccatgatt ggtgcgaccc ccaggcgatg
     6241 cagcagcggt gtgaccccgg cttcttgcac cagcgtgtcc gccacccact tgaacat
//
LOCUS       DASWZT010000055         2674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_29494, whole genome shotgun sequence.
ACCESSION   DASWZT010000055 DASWZT010000000
VERSION     DASWZT010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29494"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VGE92_01460"
     CDS             complement(<1..501)
                     /locus_tag="VGE92_01460"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEY1312509.1"
                     /translation="MAERATIARPYAKAAFEYARDAHAFAEWSQGLALAAQIIADPRV
                     ASLTKSPQWGTLQLVSLVSDVAGAKLNAGMRNFVHVLAENHRLLLLPEISAHYEELRS
                     AVENTVDVEVTSAVKLDAAQAEKLSAALSKRLKRQVRMQNSVDSTLLGGAVVRAGDLI
                     IDGSLKG"
     gene            complement(494..973)
                     /locus_tag="VGE92_01465"
     CDS             complement(494..973)
                     /locus_tag="VGE92_01465"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004411.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEY1312510.1"
                     /translation="MDIAVTLVIQGLVFFGVAWLVMKFGWPFIIGAIEARQKKIADGL
                     AAANKSQKDLDEAQARSKEIIREARDRAAQIVDQAGKRSNEMVEEAKGTAAGEAQRLI
                     AQAHDEVARETTRAREGLRQEVGKLAVEGASRLLGREIDASTHAELLDQLAAEVANG"
     gene            complement(1020..1298)
                     /gene="atpE"
                     /locus_tag="VGE92_01470"
     CDS             complement(1020..1298)
                     /gene="atpE"
                     /locus_tag="VGE92_01470"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009834415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEY1312511.1"
                     /translation="MENLAQIAQVMSATVIAAGLVIGLGGLGTAIGFGLLGGKFLESS
                     ARQPELTNMLQTKMFIVAGLLDAVPIIGVAIGLLLIYSNPFLAALGAK"
     gene            complement(1340..2179)
                     /gene="atpB"
                     /locus_tag="VGE92_01475"
     CDS             complement(1340..2179)
                     /gene="atpB"
                     /locus_tag="VGE92_01475"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004773235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY1312512.1"
                     /translation="MVQEDTAGGMTGYIKHHLTHLTVNQGHGTFWSIHLDSVVFKVAI
                     SILFVILFITVARRATSGVPGKFQAAVEIIVEFVDGMVKDTFHGSSKFIAPLAITTFC
                     LVFLENFMDMLPVDALPAAGKLFGVEHLRVVATADINTTVGMALGVFLLLWYIGIKHK
                     GVVLFFKEWFTAPFHAHGLWAVLLAPINLLLRLIEEGVRPLSLSLRLFGNMYAGELIF
                     LLIACFTLDAALNHVSTYILGGAQFIAGFIWTAFHILIITLQAFIFMTLTIVYASMAA
                     ERH"
     gene            complement(2223..>2674)
                     /locus_tag="VGE92_01480"
     CDS             complement(2223..>2674)
                     /locus_tag="VGE92_01480"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEY1312513.1"
                     /translation="SYVQIEGSAPFAGPMQSVGADLVPFAMSDVLARGNRLVIRVALL
                     QAGCAVLVASVFLVLRGSSAGLAALVGGLIVALGSVLFGWRAFRPGVAPAAVLSTAMY
                     SGVALKWLWFVVALYVALARLKLEGAPLLIGLVAAQLGYWVGLIRRK"
BASE COUNT          573 a          836 c          763 g          502 t
ORIGIN      
        1 gcctttcaat gagccgtcga ttatcagatc gcccgcccgc accaccgcac cgccgagcag
       61 agttgaatcg accgagttct gcatccgcac ctggcgcttc agccgcttgc tgagcgccgc
      121 cgaaagcttt tcggcttgcg ccgcgtcgag tttgacggcg gaggtcactt cgacgtcaac
      181 ggtattttca accgcggatc gcagctcttc ataatgcgcc gagatttccg gcagcagcag
      241 cagccggtga ttctcggcca gcacatgcac gaaattgcgc atgccggcgt tgagtttggc
      301 tcccgcgaca tcgctcacca aactcaccag ctgcaaagtt ccccactgcg gactcttggt
      361 gagcgaggcg acgcgcggat cggcgatgat ctgcgccgcc aatgccaggc cctgcgacca
      421 ttcggcaaag gcatgcgcat cgcgcgcgta ttcgaacgcg gctttcgcat agggccgggc
      481 aatggtggcg cgttcagcca ttcgctacct cggcggccaa ttggtcgagc agctcggcgt
      541 gagtgcttgc gtcgatttcg cgtcccagca gccgtgaagc gccttcgacc gcgagtttgc
      601 ccacttcttg acgcaggcct tcgcgggcgc gcgtggtttc gcgcgcgacc tcatcgtgcg
      661 cttgcgcgat gaggcgctgc gcctcgccgg cggcggtgcc tttggcttct tcgaccattt
      721 cgttggagcg cttacccgcc tggtcgacga tctgcgcggc gcggtcgcgc gcctcgcgaa
      781 tgatctcctt ggaacgcgcc tgtgcttcgt cgagatcctt ctggctctta ttggccgcgg
      841 ccagaccgtc ggcgatcttt ttttgccggg cttcgatcgc accgatgatg aacggccagc
      901 cgaatttcat caccagccaa gcgacgccga aaaacacgag tccttggatg accagcgtaa
      961 ctgctatatc cattgcctgc tcctaacagc cggcgcccgg tcgcgccgtg acgctgcgtt
     1021 tacttcgcgc ccagggcggc caagaacgga ttggagtaga tcagcaacag gccgatcgcc
     1081 acgccgatga tgggtactgc gtcgagcaag cccgcaacga tgaacatctt ggtttgcagc
     1141 atgttggtca gttccggctg gcgcgcggag ctttcgagga acttgccgcc cagaaggccg
     1201 aagccgatgg cggtacccag gccgccgagg ccgatcacga ggcctgctgc aatcacggtg
     1261 gcgctcatga cttgagcgat ttgggccaga ttttccatta ttgtctccgt cggtctttga
     1321 tgtcagtgat aaatcgaaac tagtgacgct cggccgccat gcttgcatac acgatggtca
     1381 gcgtcatgaa aatgaatgct tgcagagtaa tgatcaaaat atgaaaggcg gtccagatga
     1441 acccggcgat gaattgcgcg ccgcccaaaa tataggtgga aacgtggttg agcgcggcgt
     1501 ccagcgtaaa gcacgcgatc aacaggaaaa taagttcgcc cgcatacatg ttgccgaaca
     1561 atcgcagcga caacgacagc gggcgcacgc cttcttcaat gagccgcagc agcagattga
     1621 tcggcgccag cagcacggcc cacaagccgt gggcatgaaa cggcgcggtg aaccattctt
     1681 tgaagaacag caccacaccc ttgtgcttga ttccgatgta ccagagcagc aagaacacgc
     1741 cgagagccat gccgaccgtg gtgttgatat cggcagtggc aaccacccga agatgctcga
     1801 ctccgaaaag cttgccggcg gccggtaatg cgtcgaccgg cagcatgtcc atgaaattct
     1861 cgagaaacac caggcagaag gtggtaatcg ccaaaggcgc gatgaatttg ctgctgccgt
     1921 gaaacgtgtc cttcaccatg ccgtcgacga attccacgat gatctcgacc gccgcttgga
     1981 acttcccggg cacgccgctg gtggcgcgcc gcgccaccgt tatgaacaag atgacgaaca
     2041 ggatcgagat ggcgactttg aaaacgacgc tgtcgagatg aatcgaccaa aaagtaccgt
     2101 gaccctgatt cacggtgaga tgcgtgagat ggtgcttgat gtagcccgtc atcccgccgg
     2161 cagtatcttc ttgaaccatg tctccaaaac gcataaatcc ccgatcctta ttcaagcgat
     2221 ggtcatttgc gccgaatcaa tcccacccaa tagcctaatt gggccgcaac caacccaatc
     2281 aagagcggcg cgccttcgag cttgagccgc gccaacgcca cgtatagcgc cacgacaaac
     2341 cacagccact tcagggcgac gccgctgtac attgcagtgc tgagtactgc agccggcgcg
     2401 actccgggcc ggaacgcacg ccaaccgaac agcacgctgc cgagagccac gatgagaccg
     2461 cccaccagtg ccgcaaggcc tgcggaagaa cctctcaaca ccagaaacac tgacgcgacc
     2521 agcactgcgc agcctgcttg caggagcgcg acgcgaatta ccagccggtt acccctggcc
     2581 agtacgtcac tcatcgcgaa gggaaccaga tcggcaccga cgctctgcat cggcccggca
     2641 aacggcgcgc taccttcgat ttgcacatat gagc
//
LOCUS       DASWZT010000056         3501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_77866, whole genome shotgun sequence.
ACCESSION   DASWZT010000056 DASWZT010000000
VERSION     DASWZT010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77866"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1512
                     /locus_tag="VGE92_01485"
     CDS             <1..1512
                     /locus_tag="VGE92_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1312514.1"
                     /translation="GLEPAATQAALNDGSYIPGGTQNNISEIAPEAFTSPSSTLDLVD
                     FHGSRALLQMPGGPLTLAAGAQYVHKAQNDQDPPTVTNGIQEGITNFTVGSQDAASAF
                     VEFGGKPITSLEFDAAVRYDHYDTYGGQATPKLGLKFTPIDMLSIRGTWGKGFRAPSI
                     SESAASGIAFGEGNGSDPVLCANGVNAKGSYNALCSYPITGVEPPNPHLKAVTSTNAT
                     FGVIFEPIREFNVSLDWYHIQLKNDIINVSETGSLANYVSIARGPSVSLPVCINTPAS
                     GNCATANVLTPVGYPEYLNFPYVNAAVTTTSGIDMDLRGHFDLDVIGKFTAEFNYTYV
                     TEYDLKAGGANFELAGTHGPSSISGDTGNPRQRAVASLTWEKGPGTVTLSANYTGAFS
                     ITDPTEGWNSCLVAIQTSGVGYGAAIPATVTSLPPAFNQYCTVHHFTDFNLYGSYAVT
                     DHFAVHASIRNLFDTQPPVDLQTYGGGALFRYSTLDQDGAVGRFFLVGASLRF"
     gene            complement(1539..1612)
                     /locus_tag="VGE92_01490"
     tRNA            complement(1539..1612)
                     /locus_tag="VGE92_01490"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Cys,seq:gca)
     gene            complement(1661..1736)
                     /locus_tag="VGE92_01495"
     tRNA            complement(1661..1736)
                     /locus_tag="VGE92_01495"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1701..1703),aa:Gly,seq:gcc)
     gene            complement(1824..2426)
                     /gene="pgsA"
                     /locus_tag="VGE92_01500"
     CDS             complement(1824..2426)
                     /gene="pgsA"
                     /locus_tag="VGE92_01500"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEY1312515.1"
                     /translation="MNTSTRDAKGPSHWNLPNALTWLRILMIPAILALFYLLPYPWAD
                     PAACAAFALAGITDSLDGYYARKLGQTSRLGAFLDPVADKLIVAAALVLIVSKDSRWF
                     VVIGAVVIIGREIAVSALREWMAEIGARGRIKVSILAKYKTIMQIVGLSLLLFRQDLF
                     GIPTYKLGLGLTLIAAVLTLWSMVLYLRLAWPELRGSAEL"
     gene            complement(2401..>3501)
                     /gene="uvrC"
                     /locus_tag="VGE92_01505"
     CDS             complement(2401..>3501)
                     /gene="uvrC"
                     /locus_tag="VGE92_01505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEY1312516.1"
                     /translation="AQQIVTADIDHDADVIAMAAANGEYCVALMFVRAGRSLGSTTFF
                     PKAPWSDPPEVLAAFVAQYYLERESPPEIIVETEFDDMELLEATLAQRSGHKVRIAAS
                     VRGIRARWLEMMRNNAEQALNMRRLARASIESSLEDVREAFDLEEAPSRIECFDISHT
                     GGTDTVASCVVFGEEGPLKSEYRRFNISGIQPGDDYAAMYQALTRRYKRVRDGETPKP
                     DLLLIDGGKGQLAAAAQVLEELAVDGVTLIGIAKGADRRPGQEQLFLLGQDTPTILAP
                     DSRALRLIQRVRDEAHRFAITGHRRKRAKRHSQSILETIPGLGPVKRRELLKQFGGLQ
                     GILRAGIEDFVRIRGLGRDLAEVIYEHLHPGR"
BASE COUNT          680 a         1135 c         1056 g          630 t
ORIGIN      
        1 ggcctcgagc cggcggcgac gcaggcggcg ctcaatgacg gctcctacat tccgggaggc
       61 acacagaaca acatcagcga gatcgcgccg gaggcattca ccagcccatc gagcacactc
      121 gatctcgtcg atttccacgg atcgcgcgcg ctgctgcaga tgcccggcgg cccgctcacg
      181 ctcgcggccg gagcacagta cgtccacaag gcccagaacg accaggatcc cccgacggtg
      241 accaacggca tccaggaagg catcaccaac ttcactgtcg gctcgcagga cgcggcctcg
      301 gccttcgtcg aattcggcgg caagcccatc acatcactgg aattcgacgc cgccgtccgc
      361 tacgaccact acgacacgta tggcggccag gcgacgccga agttgggtct caagttcacg
      421 cccatcgaca tgctcagcat tcgcggcacc tggggcaagg gctttcgcgc gccgtcgatt
      481 tccgaatccg cggcgtcggg aatcgccttc ggcgagggca acggcagcga tccggtgcta
      541 tgcgcgaatg gtgtcaacgc gaagggcagc tacaacgcgc tgtgctccta tccgatcacg
      601 ggtgtcgagc cgccgaatcc gcatctgaag gccgtcacct cgaccaacgc caccttcggc
      661 gtgatcttcg agccgattcg agagttcaac gtatcgctcg actggtatca cattcagctc
      721 aagaacgaca tcatcaatgt gtccgagacg ggtagcctgg ccaattacgt atcgatcgca
      781 cgcggaccgt ccgtgtcgct gccggtttgt atcaatacgc cggcctcggg gaattgcgcc
      841 accgccaatg tcctcacgcc cgtcggatat cccgagtacc tcaatttccc ctacgtgaac
      901 gccgccgtca ccacaacttc cgggatcgac atggacctgc gcggccattt cgacctcgac
      961 gtgattggaa aattcactgc cgagttcaat tacacctacg ttaccgaata cgacctgaag
     1021 gccggcgggg cgaatttcga actggcgggg acgcacggcc cgtcgagcat ctcgggtgac
     1081 accggcaatc cgcggcagcg tgcagtggcc tcattgacct gggagaaggg accggggacg
     1141 gtcacgctgt cggcgaatta tacgggcgca ttcagcatca ctgacccgac ggaggggtgg
     1201 aactcgtgcc tggtggcgat tcaaacgtcg ggcgtcggtt acggagcggc gataccggcg
     1261 actgtgacct cgctgccacc cgccttcaac caatactgca cggtgcatca tttcaccgac
     1321 ttcaacctgt acggcagcta cgcggtgacc gaccattttg cggtccacgc gtcgattcgg
     1381 aatttgttcg atacgcagcc gccggttgac ttgcagacct acggcggcgg cgccttgttt
     1441 cgctatagca cgctcgacca agacggcgcc gtgggccgct tcttcctggt cggtgcaagt
     1501 ctgcggttct gagggcgcgc gaggcgaagc ctcggtgatg gaggctagga gcggaatcga
     1561 accgctgtac gcggctttgc aggccgctgc atgaccactc tgccacctag ccccagggtc
     1621 cgacgagtaa accccggggc ttgccggggt cttgattgac tggagcggga aacgaggctc
     1681 gaactcgcga cctcaacctt ggcaaggttg cgctctacca actgagctat tcccgcccta
     1741 aaccgaggcg caattgtagg gccctactgc ccggagtcaa gaagaactcg gcgctaaatc
     1801 gcccagcaag gcgtgcgttt cgcctatagc tcggcgctgc cgcgcaattc gggccacgcc
     1861 aggcgcaggt acagaaccat cgaccagagg gtgagaaccg cagcgatgag cgtcaaaccc
     1921 aggccgagct tgtaggtggg aattccgaac agatcttgcc ggaacaacag cagcgacagc
     1981 ccgacgatct gcatgatggt tttgtatttc gccaagatcg acaccttgat tcggccgcgc
     2041 gcgccgatct ccgccatcca ttcgcgcagc gccgacaccg caatttcccg gccgatgatc
     2101 accactgcgc cgatgaccac gaaccagcgg gaatccttgc tgacgatcag caccagggcg
     2161 gcggcgacga tcaatttgtc ggcgaccgga tccaagaaag cgccgaggcg cgaggtctgg
     2221 cccagcttac gtgcgtagta cccgtccaag ctgtcggtga tgccggccag ggcgaaagcc
     2281 gcgcaggccg caggatcggc ccacggatac ggcagcaaat agaacagtgc gaggatcgcc
     2341 gggatcatca gtatccgcag ccaggtcagt gcgttcggca gattccaatg cgatggacct
     2401 ttagcgtccc gggtggaggt gttcataaat tacctcggcg agatcgcgtc ccaaaccgcg
     2461 gattcgaacg aaatcctcaa tgcccgcacg caaaatcccc tgcaagcctc caaactgttt
     2521 caaaagctcg cgtcgtttga cgggccccag gcccggaatg gtttcaagta tcgactggct
     2581 gtgacgctta gcccgtttgc gccggtgtcc cgtgatcgcg aaccgatgcg cctcgtcgcg
     2641 cactcgttga atcaggcgca atgcccgcga gtcgggagcc agtatagtgg gcgtgtcctg
     2701 gcccaacaag aatagttgct cctggcccgg gcgacgatct gcgcccttgg cgatgccgat
     2761 taacgtaacg ccgtcgaccg ccaattcctc gagcacctgc gccgctgcgg cgagttgccc
     2821 cttgccgccg tcgatcagca acaggtcggg tttcggcgtc tcgccgtcgc ggacgcgctt
     2881 gtagcgccgc gtcagcgcct ggtacatcgc ggcatagtca tcgcccggct ggattccgct
     2941 gatgttgaag cggcgatatt cgctcttgag cgggccctcc tcgccgaata ccacacagga
     3001 agcgaccgtg tcggtgccgc ccgtatggct gatgtcgaag cactcgatgc ggctcggcgc
     3061 ctcctccaaa tcgaacgcct cgcgcacgtc ctcgagactc gattcgatgc tggcgcgcgc
     3121 caggcggcgc atgttcaatg cctgctcggc gttgttgcgc atcatctcca accaacgagc
     3181 gcgaatgccg cgcaccgagg ccgcgatgcg caccttatgt cccgagcgtt gtgccagggt
     3241 cgcctcgagc agctccatgt cgtcgaattc ggtctcgacg atgatttccg gcggcgattc
     3301 gcgctccagg tagtactgcg cgacaaacgc cgccagcact tcgggtggat cggaccaggg
     3361 cgccttcggg aagaacgtgg tgctgccgag gctgcgtccg gcacgaacga acatcagggc
     3421 gacgcagtac tcgccgttgg cggccgccat ggcgatcacg tccgcgtcat ggtcgatgtc
     3481 ggccgtgacg atctgctgag c
//
LOCUS       DASWZT010000057         3145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_87516, whole genome shotgun sequence.
ACCESSION   DASWZT010000057 DASWZT010000000
VERSION     DASWZT010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87516"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /gene="ribB"
                     /locus_tag="VGE92_01510"
     CDS             complement(<1..800)
                     /gene="ribB"
                     /locus_tag="VGE92_01510"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005108349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEY1312517.1"
                     /translation="MPAFNTIDEILEDLRRGKMAVIMDDEDRENEGDLLMAADAVRAQ
                     DVNFMARYGRGLICLTLTRERCRQLRLPLMVSESDTDGPHRTNFTLSIEAAEGVTTGI
                     SAHDRAHTVKTAVSPNASPEDLRQPGHVFPLMAQPGGVLTRAGHTEAGCDLARLAGFS
                     PAAVIVEILNDDGTMARRPDLERFAKKHDLKIGTIADLIRYRLKNERSVERIYEESVE
                     TEYGSFKLYCYEDHVNRTVHVALVKGDLNSTLPPLVRVHINETLRDVVG"
     gene            complement(1056..1223)
                     /locus_tag="VGE92_01515"
     CDS             complement(1056..1223)
                     /locus_tag="VGE92_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312518.1"
                     /translation="METALLIQAAASIAGAMAATQYGKFGGMEAHRLTEVAVIAVKIA
                     KAIELEAIKQV"
     gene            complement(1777..2382)
                     /locus_tag="VGE92_01520"
     CDS             complement(1777..2382)
                     /locus_tag="VGE92_01520"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEY1312519.1"
                     /translation="MFTGIIVASGIVDSLQEKGGDVELGVDAPDLDKNRLAIGDSISV
                     QGVCLTVIRQLETRFYADVSRETLAKTTLGELNPGSRVNLEPSLRVGDALGGHWVSGH
                     VDAVGRLRALVQDARSWRLEFELPQPLMRFVAQKGSICIDGVSLTVNNVDGRRFDVNV
                     IPHTLKVTTLGELKVEGGVNIEIDVIARYLERLSQPSSDQR"
     gene            complement(2392..>3145)
                     /gene="ribD"
                     /locus_tag="VGE92_01525"
     CDS             complement(2392..>3145)
                     /gene="ribD"
                     /locus_tag="VGE92_01525"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEY1312520.1"
                     /translation="GGIERLQAAGIAVSVGPLEAAARALNAGFYSRMERGRPFIRLKL
                     AMSLDARTAPAAPAAAGRRWITSEAARADVQCFRARSGAILTGAGTVRSDDPRLDVRM
                     AYGPWIRQPLRVLLDPMLSCPATAKLFDGGGALVFAAPDAPLRDQDRARVERLARAAG
                     GLDLHAVIARLNELEINELLVECGPRLAGSFVLSNLVDEFVLYVAPTLLGADAAPLLH
                     VGGLRAQGSLPAFEFKDVQRIGPDLRLILTPK"
BASE COUNT          582 a         1001 c          955 g          607 t
ORIGIN      
        1 cccacgacat cgcgcagcgt ttcgttgatg tgcacgcgca ccagcggcgg cagcgtcgaa
       61 ttcaagtcgc ccttcaccag ggcgacgtgc acggtgcggt tgacgtggtc ctcgtagcag
      121 tagagtttga agctgccgta ctcggtctcg acgctctctt cgtatatccg ttcgaccgag
      181 cgttcgttct tgagtcgata gcgaatcaaa tcggcgatgg tgccgatctt cagatcgtgc
      241 tttttcgcga atcgctccag atccggccgc cgcgccatgg tgccgtcgtc atttagaatc
      301 tcgacgatga ccgctgccgg cgagaatcct gccaaacgcg ccaggtcgca accggcttcg
      361 gtgtgaccgg cgcgcgtcaa cacgccgccc ggttgcgcca tcagcggaaa cacatgcccc
      421 ggctggcgca agtcctcggg actggcattg ggactgacgg ctgtcttgac ggtatgcgcg
      481 cgatcatgtg cagagatccc cgtcgtgacc ccttccgcgg cctcgatcga caacgtgaag
      541 ttggtccgat gcgggccgtc ggtatcgctc tcgctgacca tgagcggcag gcgcagctgc
      601 cggcagcgct cgcgcgtcaa ggtcaggcag atcaggccgc ggccatagcg cgccatgaaa
      661 ttcacgtcct gcgctcgcac ggcgtcggcg gccatcaaca gatcgccttc attttcgcga
      721 tcctcgtcat ccatgatgac cgccatcttg ccgcggcgta ggtcctcgag gatttcatcg
      781 atggtgttga acgcgggcat aaataacctt gtcgtcagtt ttcgtcacga acccagatac
      841 tagccgatta gattcttgta acggtctgtt attaaacgga ttatccttgg cgaaatgatc
      901 cagatgccgt cacggcggtt acgccggacc gatccgccac cctcggccgg cggcatcatt
      961 gtagcttgca gtcgcggcgt ttgattacga cgctgcacgg tgaccgtggc cgcgctccgt
     1021 ccgatagccg gatcgccgct taaggacgcc tccgactaca cctgcttgat cgcttcgagt
     1081 tcgatcgcct ttgcgatctt cacggcaatt accgcaactt ccgtcagtct gtgtgcttcc
     1141 atcccgccga acttgccgta ctgagtagct gccatcgcgc cggcaatgct ggctgcggct
     1201 tggataagta gagctgtttc cataatagat tgcgccttgg ttgtgaatcg aacgtgaaaa
     1261 agtcgggcga ccccggcgac aacaggcgac tgccggccgc gcgaaaaacg gggatttagg
     1321 gtcaaccttc ggcggaaagc cggagaacgg cggcatcagc cgctccagat cgcaggacta
     1381 aacacttgaa gagtctactc gttaacaaga agccaaaagt tgttcctttg cacggaaaca
     1441 cgacgactgc gacgcgcaca acgcgaggcc cgagaatgcg gatggtcggg atatcattgg
     1501 gggggcatgg ccacaccctt ataatttcag acttgcggcg cccggacacc ctcctttcga
     1561 agtgtgcaaa cacgcaccag ccgatttacc ctcatgaaat gcaggtaggt accctcgcgg
     1621 gcgtgatccg gggttgcttc ctgttggccg ttgccgcaac cctcgcgctg tttgcggcgg
     1681 ccgctgtcgc tgcggtgcat gagacgaccc tggctgagat gtatcacacg acctggaccg
     1741 cccgcgacgg ggcaccccag ggggttcgag cgctagctaa cgctggtcgg aggagggctg
     1801 cgaaagccgc tccaggtatc gggcgatcac gtcgatttcg atattgacgc cgccctcgac
     1861 tttgagctcg ccgagcgtcg tgactttaag cgtgtgcggg atgacgttga cgtcgaagcg
     1921 gcggccgtcg acgttgttga ccgtgaggct gacgccatcg atgcagatcg agcctttttg
     1981 cgcgacgaag cgcatcaggg gttgcggcaa ttcgaactcc agtcgccatg atcgcgcatc
     2041 ctgaaccagc gcacgcagtc gacccaccgc atcgacgtgc ccgctcaccc aatggccgcc
     2101 caacgcgtcg ccgacgcgca gactcggctc caggttcact cgggatccgg gattgagctc
     2161 acccaacgtc gttttggcca gtgtttcgcg cgacacatcc gcgtagaaac gggtctcgag
     2221 ctggcggatg accgttagac aaacgccttg cacgctgatg ctgtcgccga tagccagccg
     2281 atttttatcg aggtccgggg cgtcgacccc gagttcgacg tcgccgcctt tttcctgcaa
     2341 ggagtccact atgccgctcg cgacgattat tccggtaaac ataattaggc tttactttgg
     2401 agtgaggatc aatcgcagat cgggcccgat gcgctgcaca tccttgaatt cgaatgcggg
     2461 gaggctgccc tgggccctaa ggccgcccac atgcagcagc ggagccgcgt cggccccgag
     2521 cagtgtcgga gccacgtaca acacgaattc atccacgagg ttcgagagca cgaacgagcc
     2581 ggccaagcgc ggcccgcact cgaccaggag ttcgttgatt tcgagttcgt tcaagcgcgc
     2641 gatgacggca tgcagatcca agccgccggc ggcgcgcgcc agccgttcga ccctggcgcg
     2701 atcttgatcg cgcagcggcg catccggcgc ggcgaaaacc aatgcgccgc cgccgtcgaa
     2761 tagcttcgcc gtggccgggc aagacagcat ggggtcgagc agcacccgca gcggttgcct
     2821 gatccacggg ccgtacgcca ttcggacgtc gagccgcgga tcgtcgctgc gcaccgtacc
     2881 tgcgcccgtg agtatcgcgc cgctgcgtgc cctgaagcac tgcacgtcgg cgcgcgccgc
     2941 ctcgctcgtg atccacctgc ggcctgccgc ggccggcgcg gccggcgcgg tgcgcgcgtc
     3001 gaggctcatt gccaatttca agcggatgaa cgggcggccg cgctccatgc gcgaataaaa
     3061 gcccgcattg agcgcgcgcg ccgcggcttc caatgggccc accgacacgg caatgccggc
     3121 cgcttgcaac cgttcgatac cgccg
//
LOCUS       DASWZT010000058         6488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_63398, whole genome shotgun sequence.
ACCESSION   DASWZT010000058 DASWZT010000000
VERSION     DASWZT010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6488
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63398"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VGE92_01530"
     CDS             <1..627
                     /locus_tag="VGE92_01530"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HEY1312521.1"
                     /translation="HLDDGVISLIGDARDTWLSGGVTSWNVSFTSGAVGIDNGAAQAA
                     DAATLQTEGHYVKWNVSVYHLQNLSTRTTLYLAWSGQWANENLDSSQQMIAGGPHTVR
                     AYDMGTISGDTGNLETAEIRHDLGVACQGRWQALAFIDSEQLTINKHQFVAGTNSATL
                     SGVGLGLRWAGPAQWVAQTYLATHMGPRPSLVENPTSLRLWVEISKGF"
     gene            complement(859..2460)
                     /locus_tag="VGE92_01535"
     CDS             complement(859..2460)
                     /locus_tag="VGE92_01535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEY1312522.1"
                     /translation="MTSILQILVLLLVVIAAVAVLAKRLQIPPAILLVITGVVLALVP
                     GLPTVHLAPNFVLLFVLPPIVYSSSVAMSWREFRFSLRPISLLAVGCVLFTTIVTAAV
                     THALLGFSWSAGFVLGAIVSPPDPVAPLSIARRLQLPKRLLVILEGEGLANDASALIL
                     YRFAVAAVGASTFSLSQAAGTFAAILCAEILWGIGVGWLMLRLRRWVQDPRIEVMLSI
                     LTPFLAYWPPQHLGGSGVLATVTAGLYISWNGLRLISAATRLQGIFFWDFLMYLTEGF
                     VFLITGLQVRTLISGIHGNSLHDLTVSAAIVCGVVILARFVWMFPATYLPRWLIPSIA
                     RNDPAPPWQWPFALAFTGVRGIVSLAAALAIPLTTESGVMFPDRDRILFLTFSVILVT
                     LVGQGLMLPSVIRALGLANAGRRERHADSLEEADARRQAIEAAARRLDQLTLEHQLPE
                     AITAPLRSHLRDRRLYAERRADAAEVRRLVDRQDEIELLLIAAERQQINDLYRLGKIQ
                     DEARRRIERELDLRDAQLTNAKTEP"
     gene            2615..4210
                     /locus_tag="VGE92_01540"
     CDS             2615..4210
                     /locus_tag="VGE92_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013198953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEY1312523.1"
                     /translation="MTESEIVNEIKRRRTFAIISHPDAGKTTLTEKLLLFGGAIQMAG
                     TVKGRKATRHATSDWMRLEQQRGISVTSSVMQFPYKDCVVNLLDTPGHEDFSEDTYRT
                     LTAVDSALMVIDCAKGVEERTIKLMEVCRLRDTPIMTFVNKLDRDGRAPIELLDEIES
                     VLKIQCAPITWPIGMGRDLLGVYHLLEDRIYVYVPVEKGRIGIHETIDGIESAQAREF
                     LGARYGEFLDEVELVRGASPRFDLQRYRAAQQTPVFFGSAVNNFGVRELLSAFVSDSP
                     GPQPRPALQRQVQADEPKLTGFVFKIQANMDPGHRDRIAFMRLCSGRYTRGMRLHHVR
                     LDKDIRIADALTFLAADRSQAEEAYAGDIIGLHNHGTINIGDTFTEGEDLTFTGVPNF
                     APEMFRRAVLKDPLRMKALQKGLSQLCEEGATQLFKPLRNNDLILGAIGQLQFEVVAF
                     RLQDEYSVQCVFEPINVATARWVSLDDDKRLREFREKAYDNLAIDHAGGLVYLAPTRV
                     NLGLTEERWPEIEFLKTRENLAA"
     gene            complement(4182..6167)
                     /locus_tag="VGE92_01545"
     CDS             complement(4182..6167)
                     /locus_tag="VGE92_01545"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HEY1312524.1"
                     /translation="MTPKSVSYEQLLAIFACLALALLAHVGSLPVWVVAAVAVLGGLR
                     VLLSFRGRGAPSTLVRFSVAALTVAVLFLQFRTFNGLSAGTALLALVAGLKLFETHTQ
                     RDIYILSLCIYFLSLAALLESDSFWLLAYLIAVCWLTTATLLRLTSAAPLPSWQSSLR
                     YAARILGQALPLALAFWLFFPRLAGPLWQMPSDSGNATSGLSDSMSPGDISELALSDE
                     VAFRVHFSGTAPPAQERYFRGPVLHDFDGRTWRQGRPAQLVAPKMLLQGPAYRYTLSV
                     EPHAHNWIFALDWPARWDLPGGSLNGDFTLVEPGPVSRPIDVAATSYTHATQAQEPLS
                     GNVRRHDTQPPPGNPRSVRLAQQLRDAHPDDLDYVHAVLDMFAQQAFYYTLTPPKLAD
                     NSVDAFLFDTRRGFCGHYASAFALLMRAVHIPARVVTGYQGGTFNPFGDYWIVRQSDA
                     HAWAEVWIAGSGWLRVDPTAAIAPSRVEHGVNDAASADEPLASRWQRRTPWLMETRLR
                     LDALRQLWRERILLFDQDSQQHLLERLNIPEPDAQKLVIVMTVVLTLVFGWLTWQVRR
                     EVEASPKELLLRAYARLCAKLADAGMPRPPHEGAEDYAERIARRRPDLGPVASMLLRH
                     YSRLRYAAAPARSGVGQFDAAVRAFRPRSTPPDSRGS"
     gene            complement(6164..>6488)
                     /locus_tag="VGE92_01550"
     CDS             complement(6164..>6488)
                     /locus_tag="VGE92_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY1312525.1"
                     /translation="FAGLRAYEPGVPLKHMAWKVLARGAEPAVRSYTMSAAQPEWLDW
                     SLLEGQDTESRLSQLCLWILESEAAQRVFGLRLPGQEILPARGAAHRFACLRALAAYG
                     LAEPR"
BASE COUNT         1366 a         1997 c         2040 g         1085 t
ORIGIN      
        1 catctggacg atggagttat aagcctgatc ggtgatgcgc gcgatacatg gctttcgggc
       61 ggcgtcactt catggaatgt gagtttcacg tccggagcgg tcggcattga caatggagcg
      121 gcacaagcag cggatgcggc gaccttgcaa accgaaggcc actacgtcaa atggaatgtc
      181 agcgtttacc acctgcagaa tctgagcacg aggacgacgc tttatcttgc gtggtccggt
      241 caatgggcga atgagaatct cgattcttcg cagcaaatga ttgccggcgg accgcacacg
      301 gtgcgtgcgt acgacatggg cacgatttcc ggcgacacgg gaaatctgga aacggccgaa
      361 attcgccatg atttgggcgt agcttgccaa gggcggtggc aagcgctcgc gttcatcgac
      421 agcgagcagc tgacgatcaa caagcatcag ttcgtggccg gaacgaatag cgccacgctg
      481 agcggcgtgg gcttggggtt gcgttgggcg ggcccggctc agtgggtcgc ccagacttat
      541 ctcgccacgc acatgggccc gagaccgtca cttgtggaga atcccacgtc gttacgctta
      601 tgggtcgaga tcagcaaggg cttttgacaa cggacggatt gaggcgaagt cactgaagtc
      661 gatgttgcca attctcgcga cccgctggcg cacttaactc caacacgctg ggactgtcgt
      721 tgaacgacgg cccggccaca taaccaggtc ggcgcgcggc gcggttatta tcaggcgacc
      781 gccggccgcg aggatgccgc cgtattgcgg cgggtgttgc tgcccgacgt caagtagcgc
      841 tagcggcgct cgcggacgtc atggttcggt tttcgcattc gtcaattgtg cgtcccgaag
      901 gtcgagctcg cgctcgatcc gacgccgcgc ttcgtcttga atcttgccga ggcggtacag
      961 atcattgatt tgctggcgtt cggcggcgat cagtaacagt tcaatctcgt cctgccggtc
     1021 gacaagtcgt ctcacttctg ccgcatccgc ccgacgctcg gcatataggc gacggtcgcg
     1081 caaatgcgaa cgcaatggcg cagtaatagc ctccggcagc tggtgttcca gcgtcagctg
     1141 atcgagacgg cgcgcagcgg cctcgatggc ttgtcgccgc gcatcagcct cctccaggct
     1201 gtcggcgtgg cgctcgcggc gcccggcatt ggcaagtccc aacgcgcgaa ttacagatgg
     1261 caacatcaaa ccctgcccca caagggtcac gagaatgacc gaaaacgtca agaaaaggat
     1321 tcgatctcta tcgggaaaca tcacgccgct ctcggtggtc agcggtatcg ccagcgcggc
     1381 ggcgagcgat acgattccac gcacgccggt gaaggcgagc gcgaaaggcc actgccaagg
     1441 tggggcaggg tcgttgcgtg caatggaggg aatgagccat cgaggtagat aggtcgcagg
     1501 aaacatccag acgaagcgcg ccaatatgac gacgccacag acgatcgcag cggaaacggt
     1561 caaatcatgc aatgaattgc cgtggatacc gctaatcagg gttcgcacct gcagacccgt
     1621 gatcaagaac acgaacccct cggtcaggta catgagaaaa tcccaaaaga aaataccctg
     1681 cagccgggtc gccgcgctaa tgagccgcaa tccattccag ctgatgtaga gtcccgcggt
     1741 caccgtggcc agcacgcccg agccgcccaa gtgctgggga ggccagtacg ccaagaaagg
     1801 agtgaggatc gagagcatca cctcgatgcg tggatcttga acccaacgcc gcaatctcag
     1861 cattagccag cccacaccaa tgccccacag tatctcggcg cagagtattg cagcgaacgt
     1921 acccgccgct tgagacaacg aaaatgtact cgcgccgacc gcggcgaccg cgaaccggta
     1981 gaggataagc gcactggcgt cattggcgag cccctcaccc tcgaggatta ccaatagccg
     2041 cttcggaagc tgcagtcggc gcgctatcga cagcggcgcc acggggtccg gtggcgagac
     2101 gatagccccc aacacaaagc ccgcgctcca ggagaatccg agcagcgcat gggtaacggc
     2161 cgcggtgaca atagtcgtga acagcacgca gccgacggca agcagcgaaa tcggacgcag
     2221 actgaaacga aactcgcgcc agctcatagc gaccgacgag gaatagacaa tcggtggaag
     2281 tacgaacagc agcacgaaat tgggcgccaa atgcacggtt ggtagacccg gcacaagcgc
     2341 caagaccacc ccggttatta ccaacagaat tgccggcgga atctgcagtc gcttcgccag
     2401 cacggcaaca gccgcgatta cgacgagtaa caacacgagg atctggagaa tcgatgtcat
     2461 atcgtccccg agaaaaacac ctgactcggc tatactacgt tagggggctt aggccgagcc
     2521 gttcccgcag gtggtacctt gcgtgccact ccaactgtta tgagtatatt ttgtttcgtg
     2581 tgcgtgacgg tgtacacgcg tggggaagtg gccggtgact gaatccgaaa ttgtcaatga
     2641 aatcaagcgt cgcagaactt ttgcgatcat ctcgcatccc gacgccggca agaccacgct
     2701 gacggagaag ttgctgctgt tcggcggtgc cattcagatg gcggggaccg tgaaggggcg
     2761 caaagcgacc cgccatgcca cctccgactg gatgcgtctg gagcaacagc gcggtatctc
     2821 ggtcacctcc tcggtgatgc agtttccgta caaggattgc gtcgtcaatt tgctggacac
     2881 acccggccac gaagactttt ccgaggacac ctaccgcacc ctgaccgcgg tcgattcagc
     2941 cctgatggtg atcgactgcg ccaagggcgt tgaggagcgc accatcaagc tcatggaggt
     3001 gtgccgcctg cgcgacacgc cgatcatgac tttcgtcaac aagctcgacc gcgacggccg
     3061 cgcaccgatc gaactgctcg atgagatcga aagcgttctg aagatccagt gtgcgcccat
     3121 tacatggccg atcggcatgg gacgcgacct gctcggcgtt tatcatttgc tcgaagaccg
     3181 aatttacgtg tacgtgccgg tcgagaaggg ccgcatcggg atccacgaga ccatcgacgg
     3241 catcgagagc gcccaagcgc gcgaattctt gggagcgcgg tatggcgagt ttctcgacga
     3301 ggtggaactg gtgcgcggcg cgagtccgcg cttcgatttg cagcgctatc gagcggcgca
     3361 gcagacgccg gtattcttcg gctccgccgt caacaacttc ggcgtgagag aactgctgtc
     3421 cgcctttgtg agcgactccc cgggacctca gccgcgtccg gcgctgcagc gccaagtcca
     3481 ggcggacgag ccgaaactca cgggcttcgt tttcaagatt caggccaata tggatccggg
     3541 gcatcgcgat cggatcgcgt tcatgcgcct gtgttccgga cgatacacgc gcggcatgcg
     3601 cttgcaccat gtgcgcctcg acaaggacat tcgcatcgcc gatgcattga ccttcctcgc
     3661 cgccgaccgc agccaggccg aggaggccta tgccggcgac atcatcggac tgcacaacca
     3721 cggaaccatc aacatcggcg acaccttcac cgagggcgag gatctgacct ttaccggtgt
     3781 tccgaacttc gcgccggaaa tgtttcgccg cgccgtcctc aaggacccgt tgcgcatgaa
     3841 ggcactgcaa aaaggcctgt cgcagctgtg cgaggagggc gcaacgcagc tgttcaagcc
     3901 gctgcgcaac aatgatctga ttctcggcgc catcggccag ctgcaattcg aagttgtcgc
     3961 ttttcgatta caggatgaat acagcgtgca atgcgtgttc gagcccatca atgtggccac
     4021 cgcgcgctgg gtgagcctcg atgatgacaa gcggctgcgc gagttccgcg agaaagccta
     4081 cgataatctg gcgatcgatc atgcgggcgg gctcgtctat ctcgcgccga cgcgcgtcaa
     4141 cctcgggttg accgaggaac gctggccgga aatagaattc ctcaagaccc gcgagaatct
     4201 ggcggcgtag accttggccg gaacgcgcgc accgcggcgt cgaattgacc gacaccggag
     4261 cgcgcgggcg cagcggcata gcgcaggcgc gaatagtgac gcagaagcat gctcgcgacg
     4321 gggcccaagt cgggacggcg ccgcgcgatg cgctccgcgt agtcctccgc gccttcgtgc
     4381 ggcgggcgcg gcatcccggc atccgccaat ttggcgcaca ggcgcgcata ggcgcgcagc
     4441 agcaattcct tgggcgacgc ttcgacctcg cggcgcacct gccaggtgag ccagccgaac
     4501 accagggtca ataccacggt catcacgatg acgagttttt gcgcatccgg ctcgggaatg
     4561 ttcagccgct cgagcaggtg ttgctgcgaa tcttggtcga acagcaagat tcgctcgcgc
     4621 cacagctgac gcagggcatc gagccgcaag cgcgtctcca tgagccacgg tgtgcggcgc
     4681 tgccagcggc tcgccaatgg ctcatccgcg ctggcggcgt cattgacgcc gtgctcgaca
     4741 cggctcgggg cgatcgccgc agtgggatcg acgcgcagcc acccggagcc cgcaatccat
     4801 acttcggccc aggcgtgcgc atcgctctgc cgcacgatcc aataatcgcc gaagggattg
     4861 aatgttccgc cctgatagcc ggtcaccacc cgcgccggga tatgcacagc gcgcatcaac
     4921 agcgcgaatg ccgaggcata gtgtccgcaa aatccgcgcc tggtgtcgaa taggaatgca
     4981 tcgaccgagt tgtcggcgag cttcggcggc gtgagcgtgt agtaaaacgc ttgctgcgcg
     5041 aacatatcga ggacggcgtg cacgtagtcc agatcgtccg ggtgagcgtc gcgcagttgc
     5101 tgcgccagcc gcacgctgcg cgggttgccg ggcggcggct gggtgtcatg gcggcgcaca
     5161 ttgccgctca gcggctcctg cgcctgcgtc gcatgcgtgt atgaggtcgc cgcaacatcg
     5221 atggggcgcg acaccggtcc aggctcgacc agggtgaaat cgccgttgag cgagccaccc
     5281 ggcagatccc aacgcgcggg ccagtcgagc gcgaatatcc agttgtgcgc gtgcggttcg
     5341 acgctcagcg tatagcgata agcgggccct tgcagaagca tcttaggggc caccagctgg
     5401 gcgggacgtc cctgccgcca ggtgcgcccg tcgaagtcat gcaacaccgg accgcggaag
     5461 tagcgctcct gcgccggcgg cgccgtgccg ctaaaatgca cgcggaatgc cacctcatcc
     5521 gagagcgcga gttcagatat gtcgcccggg ctcatgctgt cgctcaaacc cgaggtggcg
     5581 ttgccgctgt cgctcggcat ctgccagagc ggcccggcca agcgtggaaa aaacagccag
     5641 aacgccagcg cgaggggtag cgcctggccg aggattcgcg ccgcataacg caggctcgac
     5701 tgccagctcg gcagtggcgc cgcgctcgtg agacgcagca gggttgccgt cgtcagccag
     5761 caaactgcga tgagataagc caacaaccag aacgaatcgc tctcgagcaa cgccgccaag
     5821 ctcaagaaat agatgcatag cgaaagaatg tagatatcgc gctgggtgtg ggtctcgaac
     5881 agtttcagcc ccgccaccag cgcgagtaac gcggtgccgg cggataggcc gttgaaggtg
     5941 cgaaactgca ggaaaagcac cgccactgtc agcgcggcga cgctgaaccg cacgagcgtc
     6001 gacggagcgc cgcgtccgcg aaagctcagc agcacgcgca gcccgcccaa cacggccact
     6061 gcggccacca cccataccgg caacgacccg acgtgcgcga gcaaggcgag cgcgaggcag
     6121 gcgaagatcg cgagaagctg ctcgtaggaa acgcttttcg gggtcaccgc ggctccgcaa
     6181 gtccgtaagc ggcaagcgcc cgcaggcagg cgaagcgatg cgctgcgccg cgcgcgggga
     6241 gaatctcctg gcccgggagc ctgagtccaa acacgcgctg cgcggcttca ctctccaata
     6301 tccagaggca taattgcgac aggcggctct cggtgtcctg cccctcgagc agcgaccagt
     6361 cgagccactc cggctgtgcg gcggacatgg tgtagctgcg aaccgccggc tcggcgccgc
     6421 gcgccaatac tttccaggcc atgtgtttca gcggtacgcc gggctcgtag gcgcgcaagc
     6481 cggcaaag
//
LOCUS       DASWZT010000059         1694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_19818, whole genome shotgun sequence.
ACCESSION   DASWZT010000059 DASWZT010000000
VERSION     DASWZT010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19818"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VGE92_01555"
     CDS             complement(<1..290)
                     /locus_tag="VGE92_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312526.1"
                     /translation="MPATLSTPFNRRLSRQALFLRALIIGAAAYAAHPAHANDATAEA
                     AEPAAAGGLEEIVVTATRHEESMSKVAISISAYTKEDMDVKGVKDISDIVRFT"
     gene            406..837
                     /locus_tag="VGE92_01560"
     CDS             406..837
                     /locus_tag="VGE92_01560"
                     /inference="COORDINATES: protein motif:HMM:NF025449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGDYG domain-containing protein"
                     /protein_id="HEY1312527.1"
                     /translation="MPLKLVSPDLCADPAVCSVVKDEIVSVEFAAAAGALDSAVGRNR
                     YAAGDALLTGSTGDRWSVSRDRFDAKYLPQAPTLQGQAGRYRNRPAAVLAKRMSVDFT
                     IERSSGGDLLRGNAGDWLVQYAPGDHGVVAAARFERVYRRT"
     gene            839..>1694
                     /locus_tag="VGE92_01565"
     CDS             839..>1694
                     /locus_tag="VGE92_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY1312528.1"
                     /translation="MSSTAERPRAKSLNPLRTLLPFLRPYRAMMAGALGSLLVATVAM
                     LALPLALREFIDHVIAAKDSSSLNGYLFGFLAAAVIFGVFAALRFYLVTWLGERVVAD
                     LRAAVYARVVRMDPLFYETTRVGEVLSRLTTDTTLVQAISGVNLSIILRSSLSLIGGL
                     VMLGLTNARLMGVMLVLIPVVVAPLIIIGRRVRTLSRASQDRIADTSGLAGETLNAIQ
                     TVQAFTLEGVQSERYRKAIEDSFKTAIRRTRVRASLTAVGIILVLAGITIVLWLGVRQ
                     VFAHTMTGG"
BASE COUNT          268 a          520 c          565 g          341 t
ORIGIN      
        1 gtgaagcgga cgatgtcgga gatgtccttg acacccttaa cgtccatgtc ctctttcgtg
       61 tacgcactga tgctgatggc gactttgctc atgctctctt catggcgggt cgcggtcacg
      121 acgatttctt cgagaccccc ggcggcggcg ggttcagcag cctccgcagt cgcgtcgttg
      181 gcgtgcgctg gatgggcggc gtaagctgct gcgccgatga tcagcgcgcg taaaaacaac
      241 gcttgccgcg aaagccgccg attgaacggc gtcgaaagcg tggctggcat ggcatccccg
      301 atgtagttat atgctcagat agctcccacc gacagtagca tcaggtaaaa accggcgcaa
      361 ccgctgaaag gacgcggcgc gacgcgtccg gtatagaatg ccgcaatgcc gcttaaactc
      421 gtgagtcccg atctttgcgc cgaccccgcc gtgtgctcgg tcgtcaagga tgaaattgtc
      481 tcggtcgaat tcgccgccgc ggccggtgcg ctcgacagcg cggtcggccg caatcgctat
      541 gcggcgggtg atgcgctcct gaccggttcg accggcgacc gctggagcgt gtcgcgcgac
      601 agattcgatg cgaaatatct gccgcaagca ccgacgttgc aaggtcaagc gggccgctat
      661 cgcaaccggc cggccgcggt gcttgcgaaa cggatgagcg tcgatttcac gatcgagcgt
      721 tccagcggcg gggatctgct gcgcggcaac gccggtgact ggctggtgca gtacgccccc
      781 ggcgatcacg gggtggtggc tgccgcgcgc ttcgagcggg tctaccggcg gacttgacat
      841 gagtagtacc gccgagcgtc cccgcgccaa gtcgctgaat ccattgcgta cgctgctgcc
      901 gttcctgcgt ccgtatcgcg ccatgatggc gggggcgctt ggctccctgc tggtggcgac
      961 cgtcgccatg ctggcgctgc cgctggcgtt gcgcgagttc atcgaccatg tcatcgccgc
     1021 caaggactcg agttccttga acggctatct gttcggattt ctcgccgcag cggtgatttt
     1081 cggtgtgttt gcggcgctgc gcttctatct cgtgacctgg ctcggcgaac gggtggtggc
     1141 ggacttgcgc gcggccgtgt atgcgcgggt ggtgcgcatg gatccgcttt tctacgaaac
     1201 cacgcgcgtc ggcgaggtgc tgtcgcgatt gaccaccgat acgacgctgg tgcaagcgat
     1261 ctccggcgtg aatctgtcga tcattttgcg atccagctta agcttaatcg gcggactcgt
     1321 catgctcggc ctgaccaatg cccggctcat gggcgtgatg ctggtgctga tccccgtcgt
     1381 ggtcgcgccg ctcatcatca tcggccggcg ggttcgcacc ctgtcgcgcg cctcccagga
     1441 ccggattgcg gacaccagcg ggctggcggg cgagacgttg aacgcgatcc agacggtaca
     1501 ggcgttcacc ttggaggggg tgcagagcga acgctaccgc aaggcgatcg aggatagctt
     1561 caagaccgcg atacggcgca cgcgcgtgcg tgcttcgctg accgcggtcg gcatcatcct
     1621 ggtgctcgcc ggcatcacca tcgtcttgtg gctcggcgtg cggcaggtgt tcgcccatac
     1681 gatgacggga ggcc
//
LOCUS       DASWZT010000060         5782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_82733, whole genome shotgun sequence.
ACCESSION   DASWZT010000060 DASWZT010000000
VERSION     DASWZT010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5782
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82733"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VGE92_01570"
     CDS             complement(<1..366)
                     /locus_tag="VGE92_01570"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY1312529.1"
                     /translation="MSELPDLSRRKFVLSTTATVAVSAAGAARSAAGCRTDDSDALCS
                     LSATEAIAHMVQGALTAERYAQALLARCQSARALNAFITLEPARVLEEARTRDRERSA
                     GAKPGPLFGLPIPVKDSVNT"
     gene            complement(423..785)
                     /locus_tag="VGE92_01575"
     CDS             complement(423..785)
                     /locus_tag="VGE92_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY1312530.1"
                     /translation="MARPLTHPHIDDVTVAGILHALSDPVRLGIVSRLLQAPAGMNCI
                     EMTQRLDVAMPKSTCSQHYRILREAGLIVSERRGVDLSSRVRAAELDRRFPGLLASIL
                     KSHKKELARAARARPVAL"
     gene            888..2009
                     /locus_tag="VGE92_01580"
     CDS             888..2009
                     /locus_tag="VGE92_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEY1312531.1"
                     /translation="MSTLFDPIKAGDLELPNRIIMSPLTRSRAGASRIPNRLMAEYYS
                     QRASAGLILSEATVVSAMGIGYAATPGIWSHEQIEGWKLTTRAVHAAGGRIFLQLWHV
                     GRVSHPSFLDGALPVAPSAVAAKGNVSLIRPPTAFVTPRALGIEEIPGIVEAFRRGAQ
                     NAQLAGFDGVELHGANGYLLDQFLQDSSNLRTDDYGGSIENRARLLLEATDAAISVWG
                     AGRVGVHLAPRGDAHSMGDSDLAGTFEYVARRLGERKIAFICAREYLGPDRLGPRLKA
                     AFGGVYIANEKFTFESAEHAVESGEADAVAFGKLFIANPDLPRRFALGSPLNEPRVPE
                     FYAGGAEGYTDYPSLAQDSQSPDLNLNHARVKARVSSGV"
     gene            complement(1946..3061)
                     /locus_tag="VGE92_01585"
     CDS             complement(1946..3061)
                     /locus_tag="VGE92_01585"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY1312532.1"
                     /translation="MTAPGNTARAAAVGIISLWFGLSSAAAAERAKVLNIYAWAEYFP
                     PALIAKFQTETGIHVNYAVLDSPETAETILSVGHSNYDLVTMNASPELAREIPRGFWK
                     KLDAASLPNARNADPQIMKLLSSVDPGNQYAVPWMWGTTGLMYNSEKIKAIMPNAPLD
                     SLDMVLKPEIAAKFAACGISMLDSWGDILPMVSRYLGQPKLSADQPQLDAVMAKLQQV
                     QRHIRRINTSGYYQQLAEGDLCLAIGYSGDAMIARRMVNESHGSVKIEYSFAREMVPF
                     YIDNLSIPADSPNPAAALAFINFVMQPEISASVTRFIGFATANAAALPFLEPAIRNNT
                     LIYPPAAVRSRFELQHVYTPEETRAFTRAWLRFKSGL"
     gene            3124..3888
                     /locus_tag="VGE92_01590"
     CDS             3124..3888
                     /locus_tag="VGE92_01590"
                     /inference="COORDINATES: protein motif:HMM:NF016818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HEY1312533.1"
                     /translation="MNSPLREIRRVRHEAKRRQLAVDKITTITPKMLRITLAGPELEG
                     FTSLGFDDHIKLFLPTPQAAPSERPPMRDYTPRYDSVAGKLHIDFAIHDAGPATDWAL
                     RARIGDTLDIGGPRGSALIPMEFDWHLLVGDETGLPAIGRRLEELPAGSRALVIAEVE
                     DEAEQQTFASHAELSVQWVHRHAAPAGSSEGLLAALRAAKFPPGDYFAWAATESNVAR
                     ALRRALIDELGAKRAWVKAAGYWQRGAAAVHERIED"
     gene            4054..>5782
                     /locus_tag="VGE92_01595"
     CDS             4054..>5782
                     /locus_tag="VGE92_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312534.1"
                     /translation="MESRFKALGTFAVLASVAAYAAVATLHHAHLHAAVAPGSAKLHV
                     FGSRSPQQRQSAAGSKYDGPLADLARHAALARPDHALEDLRSLAPGVRFQQSARGETP
                     LVLIDAVTRGDPQQLKAALVALGLQHASLYSNDVGGWLPVTQLDAATARAEVHAIRAA
                     LPHTRTGAVTSQGDFAQHSDVARSLNSLSGAGVTVGIISDSYDCYAVYAANNVPASGT
                     SGYANNGFTATAANDISTGDLPATVSVLEEAEQGNGGCMNYGAPEQLPLSDEGRAMMQ
                     IVHDVAPGAALAFYTGYNSEADFANGVGKLAASLASGGAGAKVIADDIGYFDEPFFQD
                     GIVAQAINAVAAQGVAYFSAAGNEGNSSGTNLALAYDNTVPSFATVSTAAENSGEHLL
                     NFDASGATTTTALPVTIASLIPGEFVAIVVEWDQPYVTGAPNSGGATSRIDVCLTGAS
                     GSDVIIDNAGDTATCSGPNALGSDAYQVMIVGNPATNTTNTAAETLNIVVGLADGTVG
                     PGRIKVAVEDDGAGSTINAFVTQGATIQGHPGAAGAAAVGAALYVQTPACGTTPAALE
                     YFSSQGGDPV"
BASE COUNT         1088 a         1759 c         1849 g         1086 t
ORIGIN      
        1 ggtattgacg ctgtccttca cgggtatcgg cagtccaaat agcggacccg gcttcgcgcc
       61 cgcgcttcgt tcgcggtcgc gagttcgcgc ctcctcgagc actcgggccg gttccagggt
      121 tatgaacgcg ttgagggccc gcgccgattg acagcgcgcg agcagagcct gagcgtatcg
      181 ctctgcggtc agcgcgcctt gcaccatgtg ggcgatcgcc tccgtcgcac tgagactgca
      241 cagagcgtcg ctgtcgtcgg tgcggcagcc cgcagctgag cgagcggctc ccgccgccga
      301 aacagccaca gtcgctgtgg tgctgagtac gaatttgcgg cgcgataaat cgggaagttc
      361 ggacacgatc tcgcctcagc ggttggctcg tagacaaagc ttaagattgg ccgtgccgtg
      421 ttttataagg caacaggccg cgcgcgcgcc gcgcgcgcca gctccttctt gtgggacttg
      481 aggatcgagg ccagcagtcc cggaaaacgc cggtccaatt ctgccgcccg cacccggctc
      541 gatagatcga cgccgcgccg ctcgctgacg atcaggcccg cctcgcgcaa tatgcggtag
      601 tgctgcgagc aagtcgattt cggcatcgcc acgtcaagtc tctgagtcat ctcgatgcaa
      661 ttcatgcccg caggagcctg caacagcctg cttacgatcc ccaggcgcac gggatcggac
      721 agcgcgtgca aaatgccggc aaccgtcacg tcgtcgatgt gcggatgtgt cagggggcgc
      781 gccatacctg taagaataac gcaaagcttg cattcctgat actcctatag ttcaatatta
      841 ttgaactaag cgggagcgct gcgctcgcgc atcgaacgga aggatttatg agtacgctat
      901 ttgatcccat taaagccggc gacctggaac tccccaaccg catcatcatg tcgccgctga
      961 cgcgcagccg tgccggcgcc agtcgaatcc cgaatcgatt gatggcggag tattattcgc
     1021 agcgcgcgtc cgcgggtcta atcttgtcgg aagcgaccgt ggtctcggcc atggggatcg
     1081 gttacgccgc gacaccgggc atctggtcgc atgagcagat cgagggctgg aagcttacaa
     1141 cgcgtgcggt gcacgcggcg ggcggccgga tatttttgca actctggcac gtcggccggg
     1201 tttcacatcc gagcttcctg gatggtgcgc tccccgtggc gccgagcgcc gtcgccgcca
     1261 agggcaacgt cagtctgata cggccaccga cagctttcgt cacgccgcgc gcgctcggca
     1321 tcgaggagat tcccggcatt gtcgaagcat ttcggcgcgg cgcacagaac gctcaactag
     1381 ccggattcga cggcgtcgaa ttacacggcg ccaacggcta cttgctcgat caatttttgc
     1441 aggacagcag caatttgcgc accgacgatt acggcggctc gatcgagaac cgagcgcggc
     1501 tgttgctcga agcaaccgat gcggccattt cggtgtgggg cgccgggcgt gtcggggtgc
     1561 atctggcgcc gcggggtgat gcacattcaa tgggcgatag cgacttagcg gggacgttcg
     1621 aatatgtcgc gcgccggctc ggcgagcgca agatcgcttt catttgcgcg cgcgaatacc
     1681 tggggcccga ccgcctcggc ccaaggttga aagcggcgtt cggcggcgtg tatatcgcga
     1741 acgagaaatt cactttcgaa tccgccgagc acgcggtcga atccggtgaa gccgatgcgg
     1801 tcgcattcgg caagctgttc atcgccaacc ccgatctgcc gcggcgcttt gcattggggt
     1861 cgccgctcaa tgaaccgcgg gtgccggagt tctatgcggg tggtgcggag ggctacaccg
     1921 actatccgag cctggcgcag gacagtcaaa gccccgattt gaacctcaac cacgctcgcg
     1981 taaaagcgcg cgtttcctcc ggtgtgtaga cgtgctgcag ctcgaagcga ctgcgaaccg
     2041 cagcgggcgg gtagatcagc gtattgttgc gtatcgcggg ttccagaaag ggaagcgccg
     2101 cggcatttgc ggtggcgaag ccgataaaac gggtgaccga cgcggatatc tccggctgca
     2161 tgacgaaatt gatgaacgcg agtgccgcgg cgggattcgg cgaatcggcc ggaatcgaca
     2221 agttgtcgat gtagaaaggc accatttcac gggcgaatga gtattcgatt ttcacgcttc
     2281 cgtgagattc gttcaccatg cggcgcgcaa tcatggcatc gccggagtag ccaatggcga
     2341 gacataagtc gccttccgcc agttgttggt agtaacccga agtgttgatg cgccgtatat
     2401 ggcgctgtac ttgctgcagc ttcgccatca cggcgtcgag ctgcggctgg tcggccgaaa
     2461 gcttgggctg accgaggtaa cgcgacacca tcggtaggat atcgccccag gaatccagca
     2521 tgctgattcc acaagcagcg aatttggcgg caatctcggg cttcagtacc atgtccaggc
     2581 tgtcgagcgg cgcgttcggc atgatggctt tgattttctc gctgttgtac atgaggccgg
     2641 tcgttcccca catccaagga accgcgtatt gattgcccgg atcgacgctg ctcaacagct
     2701 tcatgatttg cggatcggcg ttgcgcgcgt tcggcaaact cgctgcatcg agtttcttcc
     2761 aaaagcccct tgggatttcg cgcgccaatt ccggcgaggc attcatcgtc acgaggtcgt
     2821 agttcgagtg gccgaccgac aggatggtct ccgccgtttc gggggaatcg agcaccgcgt
     2881 aattgacgtg aatcccggtc tcggtttgga acttggcgat cagcgccggc ggaaagtact
     2941 cggcccacgc gtagatgttc aagactttgg cgcgctccgc cgcggcggcg ctgcttaaac
     3001 cgaaccagag actgatgatg ccgaccgccg ccgctcgagc ggtattgcct ggtgccgtca
     3061 tgccaatacc gccgtacaat gggaaagacg aagttcgatg atacaggcca attcggaatt
     3121 tgcatgaata gtcctcttcg ggaaatccgc cgtgtgcggc atgaagccaa acgacggcag
     3181 ctggcggtcg ataaaatcac gaccatcacc cccaaaatgc tgcgcatcac cttggcgggt
     3241 ccggaactgg aaggctttac gagcctgggg ttcgacgatc acatcaaatt attcttgccg
     3301 acgccgcaag ccgctccgtc cgagcggccg ccgatgcgcg actacacgcc gcgctatgat
     3361 agtgtggccg gaaaattgca catcgatttc gcgatacatg atgcgggacc cgcaaccgac
     3421 tgggcgctgc gcgcgcgcat cggcgatacg ctggatatcg gcggcccgcg cggatccgcg
     3481 ctgattccca tggagttcga ctggcatctg ctggtcggcg acgagaccgg cttgcctgcc
     3541 atcgggcggc gcctggaaga actgcccgcg gggtcgcgtg cactcgtgat cgcggaggtc
     3601 gaggacgagg ccgaacagca aacgtttgcg agccacgcag aattgagcgt tcaatgggtg
     3661 catcggcatg ccgcgccggc cggctcgagc gagggcttac tggcggctct gcgtgctgcc
     3721 aagtttccgc ccggcgacta ttttgcctgg gccgcaactg aatcgaatgt cgcgcgcgcg
     3781 ctgcggcgcg cgctgatcga cgaactcggc gccaagcgcg cgtgggtcaa agccgcgggc
     3841 tattggcagc gcggcgcggc ggcggtgcac gagcggatcg aggactagcg ccgatagttc
     3901 aatctgtgaa cgatttcctc gattcgcttg caggtttcct cgttaactgc gggtctgccg
     3961 cgcaacgcac gcgcgacaac gttgattgcg acacgaaact tattcggagc cggctaatct
     4021 cacaaccagg cactcgttca agggagacca aggatggaat cgcgctttaa ggctctcggc
     4081 acatttgcgg tgttggccag cgtggctgcg tacgcggccg tcgcaacgct gcaccatgcg
     4141 catttgcacg ccgccgtcgc gccggggagc gcgaagctgc atgtattcgg cagccgcagc
     4201 ccacagcagc ggcagagcgc ggcgggttcc aaatacgacg gcccattggc ggatctcgcc
     4261 cggcacgctg cgctcgcgcg tcccgatcac gctctcgaag atctcaggtc tctggcgccg
     4321 ggagtgaggt ttcagcagag cgcgcgcggc gagacgccgc tggtgctgat cgatgcggtg
     4381 acgcgcggcg acccgcagca attgaaagcg gcgctggtcg ccttgggctt gcagcacgcc
     4441 tcgctgtatt cgaacgacgt cggtggttgg cttcccgtaa cgcagctcga tgccgcaacc
     4501 gcgcgagcgg aggtccatgc cattcgcgca gcgctgccgc atacgcgcac cggtgcggtg
     4561 acctcgcaag gcgatttcgc gcaacacagc gatgtggcgc gctcgttgaa ttccctcagc
     4621 ggcgccggcg tcacggttgg gatcatctcc gacagctacg actgctacgc ggtgtacgcg
     4681 gcgaataacg tgccggcgtc gggaacatcg ggctatgcca acaatggatt cacggccacc
     4741 gccgcaaacg acatatcgac gggcgatcta cctgcgaccg taagcgtgct ggaggaagcc
     4801 gaacaaggaa acggcgggtg catgaactac ggcgcacccg aacagctgcc actgtccgac
     4861 gaagggcgcg cgatgatgca gatcgtgcat gacgtggcgc ccggcgcggc gttggcgttc
     4921 tataccggct acaacagcga ggccgacttc gccaatggcg tcggcaaact cgcagcctcg
     4981 ctcgcctcgg gcggcgcggg cgccaaagtc atcgcggacg atattggtta ttttgatgag
     5041 ccgttctttc aggacggcat cgtcgcgcag gcgatcaatg cggtcgcggc gcagggtgtg
     5101 gcgtattttt ccgccgcggg caacgaagga aattccagcg gcacgaatct ggcgttggcc
     5161 tatgacaaca ccgtgccgag ttttgccacc gtgtcgacgg cggcagagaa ttccggcgaa
     5221 catctcttga atttcgatgc ctccggcgcc accactacga ccgcgttgcc ggtcaccatc
     5281 gcatccctga tccccgggga attcgtcgcc atcgtggtcg agtgggatca accctacgtg
     5341 accggcgcac ccaacagcgg cggcgccacc agccggatcg atgtctgcct taccggggcg
     5401 agcggctcgg atgtcatcat cgacaatgcc ggcgacacgg caacctgctc cggcccgaat
     5461 gcgctgggtt ctgatgcata tcaggtcatg atcgtgggca atccggcgac caataccacc
     5521 aacaccgcgg ccgagacatt gaacatcgtc gtcgggctgg cggatggcac ggttgggccc
     5581 ggccgcatca aggttgcggt cgaagacgat ggcgcgggca gcacgatcaa tgcgttcgta
     5641 acccagggcg caaccattca agggcatccc ggcgcggccg gcgcggcggc agtgggcgcc
     5701 gctctctacg ttcagacgcc ggcttgcgga acgactcccg cggcgctcga gtatttttcg
     5761 tcccaaggcg gcgatcctgt tt
//
LOCUS       DASWZT010000061        23008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_73059, whole genome shotgun sequence.
ACCESSION   DASWZT010000061 DASWZT010000000
VERSION     DASWZT010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 23008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23008
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73059"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1413
                     /locus_tag="VGE92_01600"
     CDS             1..1413
                     /locus_tag="VGE92_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057901383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HEY1312535.1"
                     /translation="MEPTRAQLSLAEGLIQEEVGPLWEEWMREVDQVLADRELVQIVY
                     AALAHRWPNSRTRGRPGTPADVVLRLLLLKHMRNWSYHVLEREVRANLVYRQFTRVGA
                     QKVPDAKTLGKLAIALGPEIIEQIHARVVAIACEKRVIRGRRLRVDTTVVETDIHYPT
                     DSSLLGDGVRVLTRTMQRIAKIAGRAGTKLRDRTRAVGYQVRQIARASRSRVAQGQER
                     LKASYRKLLYHTARVVGQAKRFAEQIATGRKHAHGLVQQLRLEAMRAYLERMIPRVRQ
                     VMHQARERVFKGNTHVPGKLVSLFEPHTEVIRKGKASKPTEFGKMVKIQEAEAQIVTH
                     YEVYGQRPWDSDLLLPALDVHRQLLGRTPYLITADAAFFTHRNELAAQARGVKRVAIP
                     NRHTKSAQRKQLEKKRWFRNAQKWRTGCEGRISLLKRRHGLNRCRYKGLPGIERWVGL
                     GVIADNLINIGHALAPTAER"
     gene            complement(1517..2440)
                     /locus_tag="VGE92_01605"
     CDS             complement(1517..2440)
                     /locus_tag="VGE92_01605"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEY1312536.1"
                     /translation="MKYVYHPSYQIALPAGHPFPMSKYGLLKGTLVGEGIIGAADLLQ
                     PETIDHSTLQLVHTAEYLEKLAGSALSAAEQRKLGLPWSEALWLRSRLAAGGTLMAAR
                     AALAQGLSANLAGGTHHAFADHGEGFCVLNDVAVAIRRLQADRAIKRAAIIDLDVHQG
                     NGTAAIFENEDTVFTFSMHGERNYPLAKMRSNLDVPLPDGMQDAQYLDALRRHLPLVL
                     ERANADLVFYLAGVDVAAGDRYGKLALTEEGIRLRERCVIEAVRGRGLPLTIVLAGGY
                     AQTRARTAELHAHAFRECAAYERASYFPAQK"
     gene            complement(2492..2797)
                     /locus_tag="VGE92_01610"
     CDS             complement(2492..2797)
                     /locus_tag="VGE92_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312537.1"
                     /translation="MSSLDLDRQNDSPLDRVDAESTTELLERRWFAAMAAVESLKEDC
                     NIRLGIVAQAQEAWRRECAQLARIEALRDALGEQLAKHETRSLGCATAAPREAMSLR"
     gene            complement(3071..3880)
                     /locus_tag="VGE92_01615"
     CDS             complement(3071..3880)
                     /locus_tag="VGE92_01615"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEY1312538.1"
                     /translation="MVERTEQSSMRLRTVFISDIHLGFKGCSADLLLDFLHHVEMDTL
                     FLVGDIIDVWSMQKNMFWPQSHNNVLRTILGKAKRGTKVIFVPGNHDEVFREFDGAVF
                     GNLEIHCEYIHEGADGRRMLVLHGDEFDSVVKCSPWLAKLGSNIYDVLLAANPYINWV
                     RRKFDLPHWSLSSYLKNKAKTAVQYIGSFEEAVAQAARKRGVNTVVCGHIHRAEMREI
                     DGILYCNDGDWVESCTSMVEDMNGQLRLIDWPKWRERTPVVPLAVAVGQAA"
     gene            complement(4026..4232)
                     /locus_tag="VGE92_01620"
     CDS             complement(4026..4232)
                     /locus_tag="VGE92_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312539.1"
                     /translation="MKTQTHGWWLQRREASLNWLAFGLLLAAWNASDNDASRATRPRP
                     EALRGYSYVSVRAATAQLQLSRNP"
     gene            4360..5049
                     /gene="pdsR"
                     /locus_tag="VGE92_01625"
     CDS             4360..5049
                     /gene="pdsR"
                     /locus_tag="VGE92_01625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteobacterial dedicated sortase system
                     response regulator"
                     /protein_id="HEY1312540.1"
                     /translation="MKKLIAIVEDEPALRENYAAALSRHGYTVKTYANRRTAMQAFAS
                     HLPDLAIIDISLEDEAEGGFDLCRDLRALSAELPIIFLTARDSELDAVSGLRLGADDF
                     LTKDVSLPHLAARVAALFRRIDALRRPDNAAEIVRRGALSIDGEKMQTTWNGKPVTLS
                     LTEFWIVHALARTPGHVKNRQQLMDAASVVLDDNTITSHIKRVRRKFQQIDPAFDAIQ
                     TVYGMGYRWVE"
     gene            5046..7088
                     /locus_tag="VGE92_01630"
     CDS             5046..7088
                     /locus_tag="VGE92_01630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1312541.1"
                     /translation="MSIRLQLLIVALSTLVLPWAGCQYARELETALRDSQESALLASA
                     GTVANALSAQPQRVLRDPEDTTPFAAERGDWYVFPLRGAPLLDGYRDDWGVAAQPRPL
                     PAASGYSARAQLGSAGRYLYLYVEVDDTHFDPEPGNAHPERDRFDRIDLTLQHPDGTR
                     EFYFFATSAPGMIAAQSIVNGGDGEDRAVEEPRIQAFWLQTAAGYHLEARIPASFVGS
                     RLWLEAQDGSGNGKTGLDAIAAPDGGRLFSMTAGLDELLGTFIRAGTRATVIDANALK
                     LGMAGELGAVGAVGSDADTESWYRHLISVDTSSFPLQSAAPDRLEGNSVAAALAGQPH
                     AEWVRGAANQELLLTAAAPLVVAGIPRGAVFLEQAGDQLLELRDRAVSRLFNLTLLAT
                     GAAFVVMFGFATWLSLRIGRLRDAAESAVSSDGKIRLDMPESASADEIGALARGFERL
                     LARLNEHTQYLRTLGGKLSHELRTPLTIVRSSLDNLESEGLRDEQRHFVTRARDGTQR
                     LQSILSALGAAARVEESIKQAEFVNFDLCELLRSAIAGYRDGFPQSHFVLDTPADPCF
                     FRGAPDLIAQLLDKLIENAVDFCPPAGIITVRLERASGNYLLQVSNDGPPIPPALHGR
                     LFESLFEQRPGRDDKPHFGLGLYIVRLVAEFHGGGAVADNRADGGGALFTITLPLI"
     gene            complement(6975..7577)
                     /locus_tag="VGE92_01635"
     CDS             complement(6975..7577)
                     /locus_tag="VGE92_01635"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY1312542.1"
                     /translation="MNAYCYVDSPIGRLLLTTDGAALTGLYMEPSHQAQSTAGWQEDA
                     AAGALPSASRQLGEYFRGTRRDFALPLNLSGTAFQQRVWRELLDIPYGETWSYGQLAA
                     RIGKPSASRAVGLANGSNPISVIVPCHRVIGANGALTGYGGGLPRKRWLLGHEGALTR
                     GLDLDQRQSDGKERTAAVGAIVGNGAAAVKFGHQAHDVQP"
     gene            complement(7604..9034)
                     /locus_tag="VGE92_01640"
     CDS             complement(7604..9034)
                     /locus_tag="VGE92_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HEY1312543.1"
                     /translation="MELNSDACYRAVRAHDRRFDGRFFVAITSTRIYCRPICPARPAK
                     RENMRFYSSAAAAEGAGFRPCLRCRPEHAPGLASVDAVSRLVAAAIAGIEEHALSSAK
                     VGELAASLGVSDRHLRRVTESELGVSPIELAQTQRLLLAKRLLGETRLSLTEIAFASG
                     FGSVRRFNALFKSRYGLSPRDLRGTANAAAGLHCQLEFRPPFAWPSLLDFLRLRAIPG
                     VEKADATHYRRTVSIEQHDGWIAVSRAKQRNALNVEISPSLAPVIGAVIARVKRLFDL
                     GAVPDAVSALLSRDPLLANTVRRIPGLRVAGAFDGFELAVRAILGQQVSVKGATTMAG
                     RWAQRFGTPIATPYAELNRLTPTVERIAAATVDEIAALGIIGTRARSMTLLAAAVRER
                     KIALTYAPNVEEQIEALMSLPGIGPWTAQYIAMRALHWPDAFPSGDLMLLRAANATET
                     KLQKLAEDWRPWRAYATHYLWHSLAA"
     gene            complement(9095..10516)
                     /locus_tag="VGE92_01645"
     CDS             complement(9095..10516)
                     /locus_tag="VGE92_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004923742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1312544.1"
                     /translation="MADKHLQFLEVPRKDPDKLPMEVRTSEFREIYSQYGAEAAAAQA
                     GRCISCGNPFCEWKCPVHNYIPNWLALVNDGKLFEAAELSHQTNSLPEMCGRICPQDR
                     LCEGACTLNDGIGAVSIGAIEKYITDEAFKQGWKPDISNVVRTGRRVAVIGAGPAGLA
                     CADVLVRNGVTPVVFDRYSKIGGLLTFGIPPFKLEKEVVEKRREIMEYMGVEFRLNVE
                     VGRDVSFETIAEEFDAVFLGMGTYTYVKGGFAGEDLPGVHEALPYLISNINRELAIDT
                     RSSSFIDMRGKRVVVLGGGDTGMDCNRTAIRQGASSVICTYRRDEANMPGSRRDYKNS
                     KEEGVTFLFNQQPIEIVGSDRVQGVKMVETRLGAPGPRGRRIPEVVPGTEQTIDADAV
                     IIAFGFLPSPADWFVDQQIALHANGRVRVSAAPARRFQTTNPKIFAGGDMVRGSDLVV
                     TAVFEGREAAQGMLRFLGVRTAA"
     gene            complement(10536..15050)
                     /gene="gltB"
                     /locus_tag="VGE92_01650"
     CDS             complement(10536..15050)
                     /gene="gltB"
                     /locus_tag="VGE92_01650"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEY1312545.1"
                     /translation="MARKDNADLETGPGGDARAGNWGLFRSDYEKDSCGFGLIASLDD
                     QASHWVVQTAISSLNRLTHRGAIAADGKTGDGCGLLIKQPTAFLRAVAREAKLTLAPL
                     FAAGLVFLNRDADLASQARRVLTEELQREKLKLAGFRSLPVNPQACGSEALKTLPTIE
                     QVFVNAGGDIDEATFNRSLYLARRRTEKRLEGGDATFYIPSLSASTIVYKGMVMPQYL
                     VQFYPDLKDPRMEASVVVFHQRFSTNTLPQWRLAHPYRYLAHNGEINTVQGNRSWAVA
                     RGPLFRSPLLPDLSDILPVVSLKGSDSQSLDNMLELLLVGGLDPMHAMRMLVPPAWQS
                     VDMIDPDLKAFYEYYSTHMEPWDGPAGIVLTDGRYACCTLDRNGLRPARWVITKNRHI
                     TIASEAGVWDYLPEDVVKKGKLGPGEMLALDLQTSELMDTKRVDDLLKSRHPYKVWLK
                     KGVRYLQTDLIDPRLAAEPFDRDTLAVFQKMYNVSAEERDEIIRVLAEDESEAVGSMG
                     DDTPMPVLSNKVRSLYDYFRQQFAQVTNPPIDSLRESIVMSLQTQIGPECNIFVPAQQ
                     HAEQIVLSSPVLSQRKLRQIVALADSGVPNEFIDLQIDAELDLKTALSSICDQAEAAV
                     RNGKIVLLLSDRYLVKGKVPLHALLATGAVHHRLVKTGLRCKCNLLVETGTARDAHHF
                     ACLIGYGATAVYPYLAYQTLFDLMRKGALKMEAAMRQELGRSYRRGIRKGLFKIMSKM
                     GICTIASYRSAQLFEIVGLGDEVVDLCFEGSESRIQGADFADLKADADYLARRAWDPG
                     ESVEQGGLLKYMHNGEYHMYNPDVIATLQSAVMRGDYQQYREYAALVNGRPVSCIRDL
                     FKLKPQAPAIPLDDVEPLSAIFARFDSAGMSLGALSPEAHEALAIAMNRLGARSNSGE
                     GGEDPARYGTEKNSKIKQVASGRFGVTPEYLINAEVLQIKIAQGAKPGEGGQLPGHKV
                     NDMIARLRFARPGVGLISPPPHHDIYSIEDLAQLIFDLKQVNPEALVSVKLVAEPGVG
                     TVAAGVAKAYADLITISGYDGGTGASPLSSIKYAGTPWELGLAETHQTLRANDLRHRV
                     RVQTDGGLKTGLDVIKAALIGAESFGFGTAPMVALGCKYLRICHLNNCATGIATQHNV
                     LRTKYFIGLPDMVINYFKFVAQECREIMASMGVRSLAELIGHTELLEILPGETSKQRK
                     LDLKPVLANSALASSKPQFCIDPHNQPFDKGVLAERMLDDMRAAIAAKTGGEFSYAVS
                     NFHRSIGARISGEIAKRWGNTGMSLAPLIVTLKGSAGQSFGVWNAGGLHLHLEGEAND
                     YVGKGMAAGKIVLKPHARSSFVARDTVIMGNTCLYGATGGELYAAGIAGERFAVRNSG
                     ALAVVEGSGDHCCEYMTGGVVCVLGRTGVNFGAGFTGGFAYVLDMERDFVDRYNHELI
                     DIHRVSPEGMGIHVQHLRSLIEQHVDATDSVWGAQLLNDFRSYIGRFWVVKPKAAAID
                     SLLENLRRAA"
     gene            complement(15244..15672)
                     /locus_tag="VGE92_01655"
     CDS             complement(15244..15672)
                     /locus_tag="VGE92_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008050583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY1312546.1"
                     /translation="MQTVEQLLQLKGTEVFSVAPQDSVLHAIEVMATRHVGALLVMNQ
                     GALIGIISERDYARKVILKNRSSHDTPVSDIMTSPAISVTPQESVHRCMEIMTQGRFR
                     HLPVTSGGRVVGMLSIGDLVKAVIQEQSQHIEQLERYIAG"
     gene            15736..17859
                     /locus_tag="VGE92_01660"
     CDS             15736..17859
                     /locus_tag="VGE92_01660"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY1312547.1"
                     /translation="MNDQDFAPPIAAMRPHAVGSPFGVRTDPYYWLRDDERTNPEVLA
                     YLAAENAYRERRMAACKPFENALYEEIVARLKQDDSSVPYRRHGYWYYTRFEPGKEHP
                     IFARRRESLQAPEEVMLDVNELARGHDYYRIGALEVSPDSSWLAFCEDTVGRRQFSLR
                     FKNLGSGEILGTAIPDIEPDLAWANDNRTILYVEKDPATLLGLYVKKHTLGTDPRTDL
                     LAFAQTDKSFYTGVSKSKSERFIFIHMESTVSSEWQYADADDGTLAFTTFLPHERDHE
                     YQLEHLGDAFIVRTNWQARNFRLMRAAVGPGSRREQWQVVVAHREDTFIEDFDVFNNF
                     LAVAVRSGGLAKISVIPWPGNASLPGGGEFFIASDEAAYATSLSINPEIDTDLVRYVY
                     TSLTTPASVYDYNVRTGEKTLLKRDAVIGSFEPANYRTEFLWATARDGARIPVSLVYR
                     EGFVRGGRAPLLQYGYGAYGHATEPSFSAARLSLLDRGFVYAIAHVRGGQEMGRAWYD
                     AGRKLHKMNSFTDFIDVTRHLVAQGYAARDQVFAMGGSAGGLLMGAVANLSPDDYRGI
                     VAQVPFVDVLTTMLDDSIPLTTNEYDEWGDPHERADYDVMLSYSPYDNVRAQSYPALF
                     VTTGLWDSQVQYYEPAKWIAKLRALKTDANVVLLHVDMEAGHGGKAGRFQRYREIAME
                     YAFVLDQAGLRDALPAPAVAGHAAP"
     gene            complement(17992..18702)
                     /locus_tag="VGE92_01665"
     CDS             complement(17992..18702)
                     /locus_tag="VGE92_01665"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY1312548.1"
                     /translation="MNKALLGICLAVVVGLAGCSRQQSDWEKTRAANTTDSYEQFLKK
                     YPNGEFAAQAHARAKELYEERDWQKARDTDTQDAYQAFLKQYPEGKWTEEARIRVENF
                     NLAQAPSNATPAADSANNAASPAAGSAGSAATAQPPAPAPALTAKPAAAAQLYGIQLG
                     AFKSGADAANRRWAHLNTAYPTLLAGLSPIVTPKKGASGTLYRLQATGLTDQHARAIC
                     RSLKAHSQPCVVIHPAHA"
     gene            18817..19215
                     /locus_tag="VGE92_01670"
     CDS             18817..19215
                     /locus_tag="VGE92_01670"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY1312549.1"
                     /translation="MSEPKRRFFEGSSATPTFIGADSVIVGNIRGSGHFVVAGVVHGD
                     GELKGGLNLSASGSWHGFIQAEQAIVAGKITGGLTVKDKLEIGYTAVIRGKVSARTIA
                     IAKGAIVDGDIVVTSGAPILQFEEKRHGRE"
     gene            19346..20152
                     /locus_tag="VGE92_01675"
     CDS             19346..20152
                     /locus_tag="VGE92_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312550.1"
                     /translation="MIKNGLAVVTFALLANLAHADDYLSPTEERVRLSLGAMHLSSTT
                     DLRLDSSQGVTGTPLSAENDLGLDSSDFEPKFQAMVRVGERHRLRFDYFTLDRTGDTT
                     LTSPIIFRDVLLLPADPVETNLSMRTLGITYEYSFIHREKFELAATIGINDTDISARA
                     RVSTQTRHVDQEEDQAGPFPTIGLDSTYVLSKRFYVDARAQYFKIAVDHLDGSLGFYE
                     ADVVYRLRANISFALGYTAAKATLDSRQTRDSGYFDFNSKGPEFFVRVAF"
     gene            complement(20154..21491)
                     /locus_tag="VGE92_01680"
     CDS             complement(20154..21491)
                     /locus_tag="VGE92_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1312551.1"
                     /translation="MARKRALVVDDSKSARVILSRMLEKYDLEVDMAESAEQAIEYLK
                     SNRPDAIFMDHLMPGMDGLQAVQAIKSNPNTAMIPIMMYTSQEGELYVGQARALGALG
                     VLPKQVRPVDVSKVLYELHLLPERRDMTEAALTPVELENGTPVARKSAAGRETAGGDW
                     GRRLEAVLKDQALDARRFIVASLDSFASRIVNDVRGSLPSTGPLELPPPPLVQPSRPG
                     TAGIAAACLIAIGFAACWLVARDELAKSRAQVAGLTSANAELQRARLELAATVKDLTA
                     ALAAGTTGGSAPSPTNAARVEPVPYGEIPFDRGRLDALRELLTKLEAQGFHGVVKITS
                     LSGLFCLTGNATDGYAQANSTTLASKCDLVGNPFEDSLSGQQRQSLAFANLIASVRQR
                     TGGAITVEIENAGSARPSTPYPARIEALTAGEWNRAAAANNRVEFSAESAAEQ"
     gene            complement(21605..21820)
                     /locus_tag="VGE92_01685"
     CDS             complement(21605..21820)
                     /locus_tag="VGE92_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312552.1"
                     /translation="MTEEKQQKFTAKVRRGLVWVHTVAAAELAKKKAEATGVRIEGFT
                     ASELADVESALTWIAQNKESHSAEPAA"
     gene            complement(21870..>23008)
                     /locus_tag="VGE92_01690"
     CDS             complement(21870..>23008)
                     /locus_tag="VGE92_01690"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transferrin receptor-like dimerization
                     domain-containing protein"
                     /protein_id="HEY1312553.1"
                     /translation="QAIGKLVKSGWRPRRTLIYASWDGEEPGLLGSTEWTELHVAELK
                     AKAVLYINSDTNGRGYLEVAGGHALQHFASEVARDVADPETGASVLARALAREHTVSY
                     ESGARANPSGDLQLGALGAGSDFSPFLEHLGVSSLNVSFQGETEYGVYHSAYDTFEHF
                     RRFVDPTFEYGVALAKVAGRMTLRASQAQLLPAREGDFAASIAAYDEELHKLADGMRV
                     RTAELSKLLDAGAYALAADPAKPRAAPPRNAEVPYLNFAELDNAIAKLEQSAQAFDQE
                     YARIEAGDAQQAAGEREQLNAILTRLEQSLTDSKGLPGRAWYQHMVYAPGAHTGYAVK
                     TLPGIREAIEERRWEEADQYIGVVARALNAYSAQLDSAGSFRRP"
BASE COUNT         4223 a         7318 c         7124 g         4343 t
ORIGIN      
        1 attgagccca cgcgagcgca gctgtctctc gccgagggcc tgatccagga ggaagtcggc
       61 cccttgtggg aagagtggat gcgtgaggtc gatcaagtgc tcgccgaccg agagctcgtg
      121 cagattgtct acgcggcgct cgctcatcga tggcccaaca gccgtacgcg gggccggccc
      181 ggtacgcccg cggatgtggt gctgcggctg ttgctcttga agcacatgcg taactggagc
      241 taccacgtac tcgagcgcga ggtacgcgcg aacttggtct atcggcagtt cacgcgggtc
      301 ggagcgcaga aggttcccga tgccaagacc ttgggcaagc tcgccatcgc gctcggtccc
      361 gagatcatcg agcagatcca cgcccgagtg gtggccatag cgtgcgagaa gcgggtcatt
      421 cgaggccgcc ggctgcgggt agataccacg gtggtcgaga ccgacatcca ttacccgacc
      481 gacagctcgc tgctgggaga tggcgtgcgg gtgctcaccc gtacgatgca gcgcatcgcc
      541 aaaatcgccg gccgcgcagg gactaaactg cgcgatcgca cccgcgcggt gggctatcaa
      601 gtcaggcaga tcgcccgcgc cagccgcagc cgagttgcgc agggtcagga gcggctgaag
      661 gcttcctacc gtaaattgct ttaccacacg gctcgcgtgg tcggtcaagc taagcgcttc
      721 gccgagcaaa ttgccacggg gcgcaagcat gcccacggcc ttgtgcagca gttgcgcttg
      781 gaagcgatga gagcctatct tgagcggatg atcccgcggg tgcgccaggt catgcaccaa
      841 gcgcgcgaac gtgtcttcaa gggcaacacg cacgtgcccg gcaagctcgt gagcttgttc
      901 gagccgcaca ctgaggtgat ccgcaagggc aaagcctcaa agcccaccga gtttggcaag
      961 atggtgaaga tccaggaagc cgaagcgcag atcgtcacgc actacgaggt ctacgggcag
     1021 cgtccctggg acagcgattt attgcttccc gccctggatg tgcatcgcca gctcttgggc
     1081 cgcacgccgt atctgatcac cgccgacgcc gccttcttca cgcaccgtaa tgaacttgcc
     1141 gcgcaggccc gcggcgtcaa acgagtcgcc atccccaatc gccacaccaa gagtgcgcag
     1201 cgtaagcaac tcgagaagaa acgctggttc cgcaatgctc agaagtggcg caccggttgc
     1261 gagggccgta tcagtcttct caaacgtcgc cacggcctga accgctgtcg ctacaagggc
     1321 ctgcctggca tcgagcgctg ggtcggcctc ggcgtcatcg ccgacaacct catcaacatc
     1381 ggccacgcgc tcgcccccac ggccgagcgc tgacctccta cccactcgct cccttcatac
     1441 ccggccggtc gtaaggccgc ggctgacctg tcgtgctcgt gcgccggtgc cgtgcaccgc
     1501 gaccaatttc acgaggtcat ttttgcgccg gaaagtagct agcgcgttcg taggccgcgc
     1561 actcgcgaaa ggcgtgcgca tgcagctcgg cggttcgcgc tcgcgtctgc gcataccctc
     1621 cggccagcac gatggtcaac ggcagaccgc ggccgcgtac ggcttcgatc acgcagcgct
     1681 cgcgcaatcg gatcccctcc tcggtgagcg ccagcttgcc gtaacggtcg ccggcggcga
     1741 cgtcgacgcc ggcgagataa aacaccaaat cggcatttgc gcgctcgagc accagcggca
     1801 gatggcggcg cagagcatcc aggtactgcg catcttgcat cccgtcgggc agcgggacat
     1861 ccaaattgga acgcattttg gccaagggat aatttcgctc gccgtgcatc gagaaggtaa
     1921 ataccgtgtc ttcgttttcg aagattgccg cggtgccatt gccctgatgg acgtcgagat
     1981 cgatgatcgc tgcgcgcttg atcgccctgt ccgcctgcaa ccggcggatg gcgacggcga
     2041 cgtcattgag cacgcagaag ccctcgccgt ggtcggcgaa tgcgtgatgc gtgccgccgg
     2101 ccagattggc ggagagtccc tgcgcaagcg ccgcgcgcgc cgccatcagc gttcctcccg
     2161 cggcgagacg ggatctcaac cagagcgcct ccgaccaggg caggccgagc ttgcgttgct
     2221 ccgcggcgga cagggctgat cctgcgagct tttccaagta ctccgcggta tgcacgagtt
     2281 gcagcgtgga atggtcgatc gtctcgggct gcaacagatc ggcggcgccg atgattcctt
     2341 cgccgaccag cgtgcctttc aacaggccgt acttgctcat cgggaacgga tgtccggccg
     2401 gcagggcgat ttggtacgac ggatgataga cgtacttcat ccccagagta tggccgcggc
     2461 cgggcgtacg cgtccaatga gcagcgcctc gctatctgag agacatggcc tcgcgcggcg
     2521 ccgccgttgc gcagccgagg ctgcgcgttt cgtgcttcgc caattgttcg ccgagcgcgt
     2581 cgcgaagcgc ctcgatgcgt gccagctgcg cgcattcgcg ccgccaggct tcttgggctt
     2641 gggcgacgat tccgagccgt atattgcaat cctccttgag gctctcgacg gccgccatcg
     2701 cggcgaacca gcggcgctcc aggagttctg tggtggactc tgcgtctacg cgatcgagcg
     2761 gcgaatcatt ctgcctgtcg aggtcaagac tgctcatggt ttggctccta tccaagtcga
     2821 gcctcgatcc tgccgtgcga atgtgacgat ttgcggacaa cggcaagtga ttttgtttaa
     2881 tcgcagtgcc tgagccgcgt ccggcgccgt gcgcggccga gccatgagct cgcactgggc
     2941 gcgatccgct gctgtggcgg cgcattgcga cccggcgcga agccgccggc cgcgtccaca
     3001 cgaatctgcc gcgcaagact cttctgcaac cgatgccagg gcagactcca attttcgcga
     3061 atcatgtcca tcacgcggct tggccgaccg ctaccgcgag cggcacgacg ggggttcgct
     3121 cgcgccattt cggccagtcg atcaagcgta attgcccgtt catgtcttcc accatcgagg
     3181 tgcagctttc cacccagtcg ccgtcgttgc agtacaggat gccgtcgatc tcgcgcatct
     3241 ctgcgcgatg aatatggccg cagacgacgg tattgacgcc gcgcttgcgc gctgcctgcg
     3301 cgactgcttc ttcaaagctg ccgatgtatt gaaccgccgt tttggccttg tttttcaagt
     3361 acgaggacag cgaccaatgc ggcaggtcga acttgcggcg tacccagttg atgtacggat
     3421 tggcggcgag cagcacatcg tagatgttgc tcccgagctt ggcgagccac gggctgcact
     3481 tcaccacgct gtcgaattca tcgccatgca gcaccagcat gcggcggccg tccgctccct
     3541 catgaatgta ctcgcaatga atttccagat tgccgaacac ggcgccatcg aattcgcgaa
     3601 atacttcatc gtggttgccc ggaacgaaga tcaccttggt gccgcgtttt gccttgccca
     3661 agatggttcg cagcacattg ttgtgcgact gcggccagaa catgtttttt tgcatgctcc
     3721 agacatcgat gatgtcgccg accaggaaca gcgtgtccat ctcgacgtga tgcagaaaat
     3781 ccaacagcag atcggcactg cagcctttga agcccaaatg gatgtctgaa atgaagacgg
     3841 tccgcaaccg cattgacgac tgctcggtgc gctccaccat gtctcttcct cccaatgact
     3901 ttcgggtcgc gacccagaat cgtgttttgt tgttacgaag ccgtgactgg gttaagaaga
     3961 tttgcttaca aacaagtggg ttatggcgaa aacatcgcgt gaaacgatag aattatggaa
     4021 aacgtctatg gattccttga cagctgcagc tgcgcggttg ccgcgcgcac cgagacatac
     4081 gagtatccgc gcaaggcctc gggccgcggg cgcgtcgcgc gcgaggcgtc gttatccgat
     4141 gcgttccaag cggcgagcaa cagcccgaac gccagccaat tcagcgacgc ttcgcgccgc
     4201 tgcaaccacc agccatgcgt ttgtgtcttc atgatccgct cctcgttgct tgacgaggcc
     4261 agtgtgcggc gggagcagcg attccgcggg gcgcaaatca ggcaaaccgc cggccaattg
     4321 tgaccaatgg tggcgatgcc atcggctatc attccagcaa tgaaaaagct catcgcgatc
     4381 gtcgaggacg agccggcttt gcgcgaaaat tacgcggctg cgctctcgcg acacggctac
     4441 accgtcaaga cgtacgccaa tcgcaggacc gccatgcagg cgttcgcgag ccacctgccc
     4501 gatcttgcca tcatcgatat ttctctcgag gacgaagcgg agggcggctt cgatttgtgc
     4561 cgcgacttgc gcgcgctgtc ggcggagctg cccatcatct ttcttaccgc gcgggacagc
     4621 gaattggatg cggtctcggg gcttagactc ggtgccgacg atttcctgac caaagacgtg
     4681 agcctgccgc acttggcggc gcgcgttgcc gccctgtttc ggcgcatcga cgcgctgcgg
     4741 cggccggaca acgccgccga gatcgtgcgg cgcggggcgc tgtccatcga cggcgagaag
     4801 atgcagacca cctggaacgg caagcccgtc accttgtcgc tgacggagtt ttggatcgtg
     4861 cacgccttgg cgcgcacgcc gggccatgtg aagaatcgcc agcagctcat ggatgccgcg
     4921 agcgtcgttc tcgacgacaa caccatcacg tcgcacatca aacgcgtgcg ccgcaaattt
     4981 cagcaaatcg atccggcttt cgatgcaatc cagaccgtgt atggaatggg ctatcgctgg
     5041 gtcgagtgag tattcgcctg caattgttga tcgtcgcgct cagcacgctg gtgctgccgt
     5101 gggcgggctg ccaatatgcg cgcgagctcg agacggcgct gcgcgactcg caagaaagcg
     5161 ctctgctcgc gagcgcggga accgtcgcca acgctctgtc ggcgcagccg cagcgcgtgc
     5221 tgcgcgatcc tgaagacacg acgcccttcg ccgccgagcg cggcgactgg tatgtcttcc
     5281 cgctgcgcgg cgcgccgctg ctcgacggct accgggatga ttggggcgtc gcggcgcagc
     5341 cgcggccctt gccggccgcg agcggctaca gcgcgcgcgc gcagctcggc tccgccgggc
     5401 ggtatctgta tctctatgtc gaagtcgacg atacgcattt cgatcccgag ccgggcaatg
     5461 cgcaccccga gcgcgatcgc ttcgatcgga tcgatctgac gctgcagcat cccgacggca
     5521 cgcgggaatt ttatttcttc gcgaccagcg cgccgggtat gatcgccgcg caaagcatcg
     5581 tcaacggcgg cgacggtgag gaccgcgccg tggaagagcc gcgcatccag gcattctggc
     5641 tgcagaccgc ggccggctat cacctcgagg cgcgcattcc agcgagtttc gtcggctcgc
     5701 gtctctggct cgaggcgcaa gacggcagcg gcaacggcaa gacgggcctc gatgccatcg
     5761 ccgctccgga cgggggccgc ttgttctcga tgaccgcggg cctcgacgag ctcctcggca
     5821 ctttcatccg cgcgggtacc cgcgccacgg tcatcgatgc aaatgccttg aaactgggca
     5881 tggctggcga actcggcgcc gtcggcgccg tcgggtccga cgcggatacg gaatcctggt
     5941 accggcacct gatcagtgtc gacacctcga gctttccatt gcagtcggcg gcgccggatc
     6001 ggctcgaggg caatagcgtg gccgcggcgc tcgcagggca gccgcacgcc gaatgggtgc
     6061 gcggcgcggc caatcaggaa ttgctgctga cggccgccgc gcccctggtc gtcgccggca
     6121 tcccccgcgg cgcggtgttc ctcgagcagg ccggggatca actgctcgag ttgcgcgatc
     6181 gggccgtgtc gcgcttgttc aatctcacgt tgctggcgac gggcgcggcg ttcgtcgtga
     6241 tgttcggttt tgcgacctgg ctgagtctgc gcatcggacg cctgcgcgac gccgccgagt
     6301 ccgccgtgag cagcgacggc aagattcgtc tcgacatgcc ggaatctgca agcgctgatg
     6361 agatcggcgc gctggcgcgc ggcttcgagc ggctgctggc gcggctcaac gagcacacgc
     6421 aatacctacg aaccttgggc ggcaagctat cgcatgaatt gcgcacgccg ctgaccatcg
     6481 tgcgatcctc tctggacaat ctggaatccg aaggcctgcg cgacgagcaa cggcatttcg
     6541 tgacgcgcgc gcgcgacggc acccagcgcc tacagtcgat cttgagtgcg ctcggcgccg
     6601 cggcgcgggt cgaggaaagc atcaagcagg cagaattcgt gaatttcgat ttgtgcgaac
     6661 tgctgcgctc cgcgatcgcc ggctatcgcg acggctttcc ccagtcgcac ttcgtcctgg
     6721 acacgcctgc cgatccgtgc ttctttcgcg gcgcacccga cctcatcgcg cagctgctcg
     6781 acaagctgat cgaaaacgcc gtcgacttct gcccgcccgc cggaatcatc accgtgcgtc
     6841 tggagcgggc ctcgggcaac tacctgctgc aggtatcgaa cgacgggccg cccattcctc
     6901 cggcgctgca cggccgcttg ttcgaatcgt tgttcgagca gcgcccaggg cgcgacgaca
     6961 agccgcactt cggcctaggg ttgtacatcg tgcgcctggt ggccgaattt cacggcggcg
     7021 gcgccgttgc cgacaatcgc gccgacggcg gcggtgcgct ctttaccatc actctgccgt
     7081 tgatctagat cgagaccgcg cgtcagggct ccttcgtggc cgagcagcca gcgtttgcgc
     7141 ggcaagccgc cgccatagcc cgtcaatgca ccgtttgcgc cgatcacgcg atgacagggc
     7201 acgattaccg agatgggatt gctgccgttc gcgaggccca cggcacgcga ggcgctcggc
     7261 ttgccgatgc gggcagcgag ctgtccgtaa ctccacgtct ccccatacgg aatatccagc
     7321 agttcgcgcc agacgcgttg ttgaaatgcc gtgcccgaca ggttcagcgg cagcgcgaaa
     7381 tcgcgccgcg ttccgcggaa gtattcgccg agttgacgcg aggccgaagg caaggcgccc
     7441 gccgccgcgt cctcttgcca accggcggtg gattgcgcct ggtggctcgg ttccatatac
     7501 agacccgtga gggcggcgcc gtcggtcgtc agcaacagcc ggccgatcgg actgtcgaca
     7561 tagcaataag cattcattga ttcatcctca ggtggaagtc aggtcaggcg gccagggagt
     7621 gccatagata atgcgtcgcg taggcacgcc agggacgcca gtcttcggcc agcttctgca
     7681 gcttcgtctc ggtggcattc gcggcccgca gcaacatgag atcgccgctc ggaaatgcgt
     7741 ccggccaatg cagcgcccgc atcgcgatgt attgcgccgt ccaggggccg atccccggca
     7801 ggctcatcag cgcctcgatc tgctcttcga cattcggggc atacgtcaag gcaatcttgc
     7861 gttcgcgcac ggcggccgcg agcagggtca tgctacgcgc tcgcgtaccg atgatgccga
     7921 gcgcggcaat ttcatcgacc gtcgcagcgg caatgcgctc gacggtcgga gtcaggcgat
     7981 tgagttccgc atagggcgtt gcgatcgggg taccaaacct ctgcgcccac cggccggcca
     8041 tggtggttgc gcctttgacc gatacctgct gacccaagat tgctcgcacc gcgagttcga
     8101 aaccatcgaa ggcgccggcg acgcgcaagc ccggaatccg ccgcaccgtg ttcgcgagca
     8161 gcgggtcccg cgacagcagg gcgctcaccg catccgggac cgcgcccagg tcgaacaggc
     8221 gcttgacgcg ggcgatgacg gcgccgatca ccggcgccag cgagggcgag atttcgacgt
     8281 tcaaggcgtt gcgctgcttg gcccgcgaca ccgcgatcca gccgtcgtgc tgctcgatcg
     8341 acaccgtgcg ccgatagtgc gttgcatccg ccttctcgac cccggggatg gcacgcagcc
     8401 gcagaaagtc gagcaggctc ggccaggcaa acggcggcct gaattccagc tggcagtgca
     8461 agcctgcggc ggcattcgcc gtgccgcgca gatctcgcgg actcaatcca tagcgcgatt
     8521 tgaacagtgc attgaagcgg cgcacgctgc caaagccgct cgcaaacgcg atctcggtca
     8581 ggctcaggcg tgtttcaccg agcaggcgct tcgccaacag cagccgctgc gtctgtgcga
     8641 gttcgatcgg cgataccccg agctccgatt cggtaacgcg ccgcaggtgg cggtcactga
     8701 cgccgagcga ggccgccagc tcgccgacct tggcgctcga cagcgcatgt tcctcgatgc
     8761 ccgcaatcgc cgccgcgacc aggcggctga cggcatcgac ggatgcgagt cccggcgcgt
     8821 gctccggccg gcaccgcagg cacggcctga atcccgcgcc ttccgcggcg gcggcgctcg
     8881 aataaaagcg catgttctcg cgcttcgccg gccgcgccgg acaaatggga cggcaataaa
     8941 tgcgggtgga ggtgatcgcc acgaagaagc gcccgtcaaa gcggcggtcg tgggcgcgca
     9001 ctgcgcgata acaagcgtcg gagttcaatt ccatggtgcc aggatagcca aatcgcgccg
     9061 ccaatactcg ccgttttcgg acctctaaat atgctcaggc ggcggtgcgc acccccaaaa
     9121 agcgcagcat cccttgtgcg gcttcgcggc cctcgaacac cgcggtcacc accaaatccg
     9181 agccgcgcac catgtcgccg ccggcgaaga tctttggatt cgtggtctga aaccgccgtg
     9241 ccggcgcagc cgacacgcgt acgcggccgt tggcgtgcaa cgcgatctgc tgatcgacga
     9301 accagtccgc ggggctcggt agaaatccga aggcaatgat gacggcgtcg gcatcgatgg
     9361 tctgttccgt cccgggaacg acttccggga tgcggcggcc gcgcgggccg ggtgcaccca
     9421 agcgggtctc gaccatcttg acgccctgca cccgatccga accgacgatt tcgatcggct
     9481 gctggttgaa taaaaaagta acgccttcct ccttgctgtt cttgtagtcg cgtcgcgagc
     9541 ccggcatgtt ggcctcgtcg cgacggtagg tacagatgac gctgctcgcg ccctggcgga
     9601 tcgccgttcg attgcagtcc atgccggtgt cgccgccgcc gagcacgacc actcgcttgc
     9661 cgcgcatgtc gatgaaagaa ctcgaacggg tatcgattgc caattcgcga ttgatgttgg
     9721 aaatcagata cggcaacgcc tcatggaccc ccggcagatc ttcgccggcg aatccaccct
     9781 tgacgtaggt gtaagtgccc atgccgagaa acacggcgtc gaattcttcc gctatcgtct
     9841 cgaaagacac gtcgcgtccg acctcgacat tcaggcggaa ctccaccccc atgtattcca
     9901 tgatctcgcg gcgcttctcg acgacttctt tttccaattt gaacggcggg atgccgaagg
     9961 tgagcagacc gccgattttc gagtagcggt cgaacaccac cggcgtcacg ccgttgcgga
    10021 ccagcacgtc ggcacatgca agcccggcgg ggcccgcgcc gatgacggca acgcgcctgc
    10081 cggtgcgcac cacattcgag atgtcgggct tccaaccctg cttgaatgcc tcatccgtga
    10141 tgtatttttc gatggcgccg atactgacgg cgccgatacc gtcattcaag gtgcaggctc
    10201 cttcgcacag gcggtcctgc ggacagatgc ggccgcacat ctccggcaga gaattggttt
    10261 gatgcgacag ttcggccgct tcgaacagct tgccgtcgtt caccagcgcc agccagttcg
    10321 gtatgtaatt gtgcaccgga catttccatt cgcagaatgg gttgccgcag gaaatgcagc
    10381 ggccggcttg ggcggcggcg gcctcggcac cgtattgcga atagatctcg cgaaactcgc
    10441 tggtgcgcac ttccatcggc agcttgtccg gatctttgcg cggaacctcg aggaattgca
    10501 ggtgtttgtc ggccacgggt gaagctacag gcgcctcaag cggcgcgccg cagattttcc
    10561 agcagcgaat cgatggccgc ggctttcggc ttcaccaccc agaagcggcc gatgtagctg
    10621 cgaaaatcgt tgagcaattg cgcgccccag acgctgtccg tggcgtcgac gtgctgttcg
    10681 atgagactgc gcaagtgctg cacatgaata cccatgcctt ccggggaaac gcggtggatg
    10741 tcgatcagct cgtggttgta gcggtcgacg aagtcgcgct ccatgtcgag cacataggca
    10801 aagccgccgg tgaatccggc gccgaaattc actccggttc gcccgagcac gcacaccacg
    10861 ccgccggtca tgtattcgca gcagtgatcg ccgctgccct ccaccaccgc gagcgcgccg
    10921 gaattgcgca ccgcgaagcg ctccccggcg atacccgcgg cgtacagctc gccgccggtg
    10981 gctccgtaca aacaggtgtt gcccatgatc acggtatcgc gcgccacgaa actcgagcgc
    11041 gcatgcggct tcaagacgat cttgcccgcc gccatgcctt tgccgacgta gtcattggcc
    11101 tcgccctcga gatgcagatg caagccgccg gcgttccaca cgccgaaact ctgtccggcc
    11161 gagcccttca gagtgacgat gagcggcgcc aggctcatgc cggtgtttcc ccaacgcttg
    11221 gcgatttcac cggaaatccg cgcgccgatg gagcggtgaa aattgctcac tgcataggag
    11281 aattcgccgc ccgtcttcgc ggcgatggcc gcgcgcatgt cgtcgagcat tctttcagcg
    11341 agcacgcctt tgtcgaacgg ctggttgtgc gggtcgatgc agaattgcgg cttgctcgat
    11401 gccagcgccg agttcgcgag caccggctta agatcgagtt tgcgctgctt ggaggtctcg
    11461 cccggcagaa tttccagcag ttccgtgtgt ccgatcagct cggccaagct gcgcacgccc
    11521 atggatgcca tgatttcccg gcactcttgc gcgacgaatt tgaaataatt gatcaccatg
    11581 tcgggcagcc cgatgaaata cttggtacgc agcacgttgt gctgcgtggc gatacccgtg
    11641 gcgcagttgt taaggtgaca gatccgcaga tatttgcagc ccaacgccac catcggcgca
    11701 gtgccgaagc cgaagctttc ggcgccgatg agcgcggcct tgatcacatc gagaccggtc
    11761 ttgagtccgc cgtcggtctg cacacgcacc cggtgcctta agtcattggc gcgcaacgtc
    11821 tgatgcgttt ccgcgagtcc caattcccac ggcgtgccgg cgtacttgat cgatgacagc
    11881 gggctcgcgc cggtgccgcc gtcatagcct gagatggtga tcaaatccgc atatgccttg
    11941 gcgaccccgg cggcgaccgt gccgacgccg ggctcggcca ccagcttcac cgataccaac
    12001 gcctcgggat tgacctgctt taagtcgaat atcaactgcg cgagatcctc gatggagtag
    12061 atgtcgtggt gcggcggcgg cgaaatgaga ccgacgcccg gccgcgcaaa tcgcaggcgc
    12121 gcgatcatgt cgttgacttt gtgcccgggc agctgcccgc cttcacccgg tttcgcgcct
    12181 tgcgcgattt tgatctgcag tacctcggcg ttgatcaagt attccggagt gacaccgaag
    12241 cggcccgagg cgacctgttt gatcttggaa ttcttttccg tgccatagcg cgcgggatcc
    12301 tcgccgccct cgcctgaatt ggaacgggca ccgaggcgat tcatcgcaat cgcgagcgcc
    12361 tcgtgcgcct cgggcgacag tgcgcccaac gacatgccgg cgctgtcgaa gcgcgcgaaa
    12421 atggccgaca gcggttcgac gtcgtcgagc ggtatggccg gagcctgcgg cttgagtttg
    12481 aacagatcgc gaatgcacga caccggccgc ccattcacca aggcggcgta ctcgcgatat
    12541 tgctgatagt cgccgcgcat caccgccgac tgcagggtgg cgatcacgtc cgggttgtac
    12601 atgtggtact cgccattgtg catgtacttg agcagaccgc cctgctcgac gctttcaccc
    12661 ggatcccagg cgcgccgcgc cagatagtcg gcatcggcct tgagatcggc gaaatccgcg
    12721 ccttggatgc ggctctcgga gccctcgaag cacaggtcga cgacttcatc gcccaagccc
    12781 acgatctcga acaattgcgc actgcggtag cttgcgatcg tgcaaatgcc catcttggac
    12841 atgatcttga acagtccctt gcggatgccg cgccgatagc tgcggccgag ctcctggcgc
    12901 atggcggctt ccattttcag cgcgcccttg cgcatcaggt cgaacaaggt ctggtaggcg
    12961 agatacggat acacggcggt cgcgccatag ccgatcaaac acgcaaagtg atgcgcgtcg
    13021 cgcgcggtgc cggtctccac caacagattg cacttgcagc gcaggccggt cttcaccaaa
    13081 cgatggtgca ccgcgccggt ggcgagcagc gcgtgcagcg gcaccttgcc cttcacgagg
    13141 tagcgatccg acagcagcag cacgatcttg ccgttgcgca ccgccgcctc cgcctgatcg
    13201 cagatgctcg acagcgcggt cttcagatcg agctcggcgt cgatttgcaa gtcgatgaac
    13261 tcgttcggca cgccggaatc ggcgagcgcc acgatctgcc gcaatttacg ctgcgacaac
    13321 accggcgagg agagcacgat ttgctcggcg tgctgctgcg cgggcacgaa gatgttgcac
    13381 tcggggccga tctgcgtctg cagcgacatg acgatgcttt cacgcaggct gtcgatcggc
    13441 ggattcgtga cttgcgcgaa ctgttgccgg aaataatcgt acagcgagcg taccttgttc
    13501 gacagcaccg gcatgggggt gtcatcgccc atcgaaccca ccgcttcgct ttcgtcttcg
    13561 gcgagcacgc ggatgatctc gtcgcgttct tcggccgata cgttgtacat cttctggaac
    13621 accgccaggg tgtcgcgatc gaacggttcg gcggccaagc gcgggtcgat cagatcggtt
    13681 tgcaggtagc gcacgccctt cttgagccac accttgtagg gatggcggct cttcaagaga
    13741 tcatcgaccc gcttggtgtc catgagctcg gaggtttgca aatcgagcgc cagcatctcg
    13801 cccgggccca acttaccctt cttgaccaca tcttcgggca ggtagtccca gacgccggct
    13861 tccgaggcga tggtgatatg tcgatttttc gtgatgaccc aacgcgccgg gcgcagacca
    13921 ttgcgatcga gcgtgcagca ggcgtaacga ccgtcggtga gtacgattcc cgccggcccg
    13981 tcccagggct ccatgtgcgt cgagtagtac tcgtagaacg ctttgaggtc ggggtcgatc
    14041 atgtccaccg actgccacgc cggcggcacc agcatgcgca tggcgtgcat cggatccaaa
    14101 ccgccgacga gcagcagctc gagcatgttg tcgagcgact gcgaatcgga gcccttcaag
    14161 gacaccaccg gcagaatgtc gctcaagtcc ggcagcagcg gtgagcgaaa cagggggccg
    14221 cgcgccaccg cccaactgcg gttgccttgc acggtgttga tttcgccatt gtgagccaag
    14281 taacgatacg gatgagcgag gcgccactgc ggcagggtgt tggtcgagaa gcgctggtgg
    14341 aaaaccacca ccgaggcttc catccgcgga tccttcaggt cgggatagaa ctgcaccagg
    14401 tactgcggca tgaccatgcc cttgtagacg atggtgctgg ccgataatga cgggatatag
    14461 aacgtcgcat cgccgccttc gagccgcttc tcggtgcggc gccgtgccaa atacaaactg
    14521 cggttgaagg tggcctcgtc gatgtcacca ccggcgttga cgaaaacctg ctcgatggtc
    14581 ggcagggttt tgagcgcttc ggagccgcag gcctgcggat tcacgggcaa gctgcgaaaa
    14641 cccgcaagct tgagtttctc gcgctgcagc tcttcggtga gcacgcgccg cgcctgcgat
    14701 gcgaggtcgg catcgcgatt gaggaacacc aagcccgcgg caaatagcgg tgccagtgtc
    14761 agcttggctt cccgagccac ggcacgcagg aaagcggtgg gctgcttgat cagaaggccg
    14821 cagccatctc cggtcttgcc gtcggcggca atcgcaccgc gatgggtcaa acgattcagc
    14881 gagctgatcg cagtctgtac cacccagtgg ctcgcctggt catcgagact ggcgatcaga
    14941 ccgaagccgc aggagtcctt ttcatagtcc gaacggaaca gaccccagtt gcccgcgcgc
    15001 gcatcgccgc ccgggccggt ctcaagatcc gcattgtcct ttctcgccat agcgcgcgcc
    15061 catgcaaaca caaagccctg cagttcactg taggaccttt gaaaaacaac aacttaaatc
    15121 ggttctagac acccgatggt cgagtgccag gtaagggcaa acgccgctaa gcgcgcctta
    15181 agtatgcaag tcatgatacc tagcccgcga ttagcgtcaa tcgcggattc ggcgacgccg
    15241 cggttagccg gcgatgtagc gctccagctg ttcgatatgc tggctctgtt cttggatgac
    15301 ggctttgacc agatcgccga tcgacagcat tccgacgacg cgcccgcccg aggtgaccgg
    15361 caaatgccgg aatcgtccct gggtcatgat ctccatgcag cggtgcacgg actcctgcgg
    15421 cgtgacgctg atggcgggcg aggtcatgat gtcgctcacc ggggtgtcat gcgaggagcg
    15481 gtttttgagg atgactttgc gggcgtagtc gcgctcggaa ataatgccga tgagcgcgcc
    15541 ttgattcatc accagcagtg cacccacgtg ccgggtcgcc atgacctcga tcgcgtgcaa
    15601 caccgagtcc tgcggagcga cggagaagac ctcggtgcct ttcaattgaa gcaactgttc
    15661 cacggtctgc atacgccccc ccgatggctc tgactatact ccggcctagt ctgtggacaa
    15721 gtggtactca tgtcgatgaa cgatcaagat ttcgccccac ccatcgcggc catgcggccg
    15781 catgccgtcg gctcgccgtt cggcgtcaga acggacccct attactggtt gcgggacgat
    15841 gaacgcacca atccggaggt gctcgcctac ctcgcggccg agaacgccta tcgcgagcgc
    15901 cgcatggccg cgtgcaagcc gttcgagaac gccttgtacg aggaaatcgt cgcgcgcctg
    15961 aagcaggacg atagcagcgt gccgtatcgc aggcacggct actggtatta cacgcgattc
    16021 gagcccggca aagagcaccc tattttcgcg cggcgcaggg agtcgctgca ggcgccggaa
    16081 gaggtcatgt tggacgtcaa cgaactcgcc aggggtcacg attactatcg aatcggcgca
    16141 ctcgaagtct cgccggactc ctcgtggctg gcgttttgcg aggacacggt cggccggcgt
    16201 caattcagct tgcgcttcaa gaatttgggt agcggtgaaa tcctcggcac tgcgattccc
    16261 gatatcgagc ccgacctcgc ctgggccaac gacaatcgca ccatcctgta cgtcgaaaag
    16321 gatcccgcga cgctgctcgg tctttatgta aaaaagcaca ccttgggaac ggacccgcga
    16381 accgacctgt tggcattcgc acaaacggac aagagtttct ataccggcgt atcgaagtcg
    16441 aaatccgagc gtttcatctt catccacatg gaaagcacgg tgtcctcgga atggcaatac
    16501 gccgatgccg acgatggcac gctcgcgttc acgaccttcc tgccgcacga gcgcgatcat
    16561 gagtaccagt tggaacattt gggcgatgcc ttcatcgtcc gcaccaattg gcaggcacgc
    16621 aatttccgtt tgatgcgcgc agccgtcggc ccggggagtc gccgcgagca atggcaagtt
    16681 gtggtcgcac atcgcgagga taccttcatc gaagacttcg acgtgttcaa taattttctc
    16741 gccgtggcgg tgcgtagcgg cggtttggcc aagatcagcg tcatcccgtg gcccggcaat
    16801 gcgtcgctgc cggggggcgg ggaatttttt atcgccagcg acgaggccgc ctacgcgaca
    16861 tcgctgtcga tcaatcctga gatcgacacc gatctcgtgc gctacgtgta cacctccctc
    16921 acgacgccgg cgagcgtcta cgactacaac gtgcgcacag gagagaagac tctgctgaag
    16981 cgagatgcgg tcatcggcag cttcgaaccc gcgaattatc gaaccgaatt cttgtgggcg
    17041 acggcgcgcg acggcgcacg gatacccgtc tcgttggtgt accgcgaggg cttcgtgcgc
    17101 ggcggtcgcg caccgttgct gcagtatggc tacggcgcct acgggcatgc caccgaaccg
    17161 agtttttcag cggcgcgcct cagtttgctc gaccgcggtt tcgtatatgc gatcgcccac
    17221 gtgcgcggcg gtcaggaaat gggccgcgcc tggtacgatg cgggacgcaa gttgcacaag
    17281 atgaattcgt tcacggattt catcgacgtg acgcgccact tggtcgcgca gggttacgcg
    17341 gcaagagacc aggtgtttgc catgggcggc agcgccggcg gcctcttgat gggcgccgtg
    17401 gccaacttga gtccggacga ttaccgcggc atcgtcgcgc aagttccgtt cgtcgacgtg
    17461 ctcaccacca tgttggacga cagcattccg ctgaccacga atgaatacga tgagtggggc
    17521 gacccccacg agcgcgccga ctatgacgtc atgctgtcct attcgcccta tgacaatgtt
    17581 cgagcgcaga gctatcccgc cctgttcgtc acgacggggc tttgggacag ccaggtgcag
    17641 tactacgagc cggccaagtg gattgccaag ctgcgcgcgc tgaagaccga tgccaatgtc
    17701 gtgctgttgc atgtcgacat ggaggcgggt cacggcggta aggcgggccg ctttcaacgt
    17761 taccgcgaga tcgcgatgga gtacgccttc gtgctcgacc aggcggggct tcgcgatgcg
    17821 ctgcctgcgc ccgccgtggc gggccacgca gccccctagg ctgatgagtt acgagacgtc
    17881 ctgcacgtcc cgacgacagc gggccgtcgc agggttaaaa cttgaataaa ttcaattagc
    17941 tgttgtccac gaggtctcgt cgaccgacaa gcattgtcca aaggtgacgc gctacgcatg
    18001 cgccggatgg atgaccacac agggctgcga atgagccttc aaggatcgac aaatcgcgcg
    18061 cgcatgctgg tcggtcagac cggttgcttg gaggcgatat agggtccccg acgccccttt
    18121 cttgggcgtc acgatcggcg aaagcccggc caagagcgtc ggatacgccg tgttcaagtg
    18181 cgcccaacgc cggttcgccg catcggcgcc cgacttgaat gccccgagct ggatgccgta
    18241 aagctgcgcc gcagccgcag gcttcgcggt cagcgcgggt gctggagccg gcggctgcgc
    18301 agtcgcggcc gaccccgcgc tgccggccgc cggacttgcg gcgttattcg cggagtctgc
    18361 ggcgggagtg gcgttcgacg gcgcctgggc caggttgaaa ttctcgaccc ggatgcgcgc
    18421 ctcctcggtc cacttaccct cgggatattg cttgaggaat gcttgatagg cgtcttgcgt
    18481 atcggtatca cgcgcttttt gccaatcgcg ttcttcatag agctctttgg cccgagcgtg
    18541 cgcctgcgcg gcgaattcac cgttcgggta cttcttcaaa aactgctcgt aggaatcggt
    18601 cgtgttcgcg gcccgagtct tttcccaatc gctttgttgg cggctgcaac cagcaaggcc
    18661 gaccacgacc gcaagacaaa tccccaaaag cgctttgttc ataaccatgt ttacactcgg
    18721 aattcgcgtg aggctgcatt ctacttcgat tcgcgatgtc gcggcgacgc caagctcgtc
    18781 acatcacggg atgctgcgga taggataata ggccccatga gcgaaccaaa acgacgcttc
    18841 ttcgagggca gctcggcgac acccactttc atcggcgccg actcggtgat cgtcggcaat
    18901 attcgcggca gcggacactt cgtcgtggcc ggagtggtgc acggcgacgg cgagctcaag
    18961 ggcgggctca atctgtccgc ctcgggaagc tggcacgggt tcatacaagc agagcaggcg
    19021 atcgtcgccg gcaaaatcac cggaggtctc acggtcaagg acaaactcga aatcgggtat
    19081 accgccgtga ttcgcggcaa ggtcagcgcg cgaaccattg cgatcgcgaa aggcgccatc
    19141 gtcgatggcg atattgtagt cacatcggga gcgccgatcc tgcagttcga agaaaagcgc
    19201 cacggcaggg aatgactgaa attcgggctg ttcgcgaatg gcgacagaga cgcaaaagtg
    19261 gtttgttcca gtgtattccc gcatcctagc gcagattcgt cgcaggcgag ccgcaacgga
    19321 ccaatcgatt ctgaggagac cgacaatgat caagaacggc ctggccgtgg tgacgttcgc
    19381 gttgctcgcg aaccttgcgc acgccgacga ctacttgagc ccaactgagg agcgcgtgcg
    19441 gctgtcgttg ggcgcgatgc acctgtcatc gacgaccgac cttcggttgg acagcagcca
    19501 gggcgtcacc ggtacgccgc tctccgcgga gaacgatctc ggcctggaca gctccgactt
    19561 cgaacccaaa tttcaggcca tggtgcgcgt cggcgagcgg catcgactgc gcttcgatta
    19621 ttttacgttg gatagaaccg gcgataccac gctcacttcg ccgatcatct ttcgcgacgt
    19681 gctgttgctg cccgccgatc ccgtggaaac caatctgagc atgcgcacgc tcggcatcac
    19741 ctacgaatat tcattcatcc atcgcgagaa attcgaactc gccgcaacca tcgggatcaa
    19801 tgacaccgac atctcggccc gggcgcgcgt gtcgacgcaa actcgacacg tcgatcaaga
    19861 ggaagaccag gcgggtccgt tcccaaccat cggcctcgac tcgacctacg tcttgagcaa
    19921 gcggttctac gtcgatgccc gcgcacagta tttcaagata gccgtcgacc accttgacgg
    19981 ctcgctcgga ttctacgaag cggacgtggt ctaccggttg cgcgccaaca tctcgttcgc
    20041 gttgggttac accgcggcga aagcgaccct tgactcgcgc caaacccgcg attcagggta
    20101 tttcgatttc aactcgaaag gcccggaatt cttcgtcaga gttgcctttt aggtcactgc
    20161 tccgcggcgg attcggcgga gaattccacg cgattattcg cggcggccgc gcgattccac
    20221 tcgccggcgg tcagcgcctc gatgcgcgcc ggatagggag tactggggcg cgcgctgccc
    20281 gcgttttcga tttccacggt gatcgcgccg ccggtgcgtt ggcgcacact ggcgatcaaa
    20341 ttcgcaaacg ccaacgattg gcgctgctgg cccgataacg aatcttcgaa cgggttgccc
    20401 accagatcgc acttgctggc caaggtggtt gagttcgcct gcgcatagcc atcggtcgcg
    20461 ttgccggtga gacaaaacaa tcccgacagg ctggtgattt tcaccacgcc gtgaaatccc
    20521 tgcgcttcga gtttggtcag aagctcgcgc aacgcgtcga ggcggccgcg atcgaaggga
    20581 atttccccgt acggaaccgg ttccactcgt gccgcattgg tgggtgacgg cgcgctgccg
    20641 ccggtggtac cggccgcgag tgcggcggtg aggtctttca cggtcgcggc aagctcgagg
    20701 cgcgcacgct gcaattcggc attggccgac gtcaagccgg cgacctgcgc gcgcgatttc
    20761 gccaactcgt cgcgcgcgac cagccaacag gccgcgaagc cgatggcaat cagacacgcc
    20821 gccgcaatgc cggcggtgcc gggacgggac ggctgcacga gaggcggcgg cgggagctcc
    20881 aagggtcccg tggacggcaa ggaaccgcgt acatcgttga cgatccgcga ggcaaagctg
    20941 tcgagactcg cgacgatgaa gcggcgcgca tcgagcgcct gatccttcag caccgcttcc
    21001 aagcgacggc cccagtcgcc gccggccgtt tcccggccgg ccgcggattt gcgggcgacc
    21061 ggcgtgccat tctccagttc caccggcgtc agcgccgctt ccgtcatgtc gcgacgctcc
    21121 ggtaacaaat gtaattcgta caatactttc gacacatcga ccgggcgcac ctgcttcggc
    21181 agcacgccca gcgcgcccag cgcccgcgcc tgccccacgt acagttcgcc ctcctgcgag
    21241 gtgtacatca tgatcgggat catggcggta ttcgggttgc ttttgatcgc ctgcactgct
    21301 tgcaagccat ccatccccgg catgagatgg tccatgaaga tggcgtcggg gcgattactc
    21361 ttcaaatact cgatcgcctg ctcggcggat tcagccatgt cgacttcgag gtcgtacttc
    21421 tcgagcatgc ggctcaagat gacgcgagct gacttggaat cgtcgacgac gagtgcgcgt
    21481 tttcgggcca ttccgctagt gtatcgcagt cctatcaata ccggatggag cggcttcggg
    21541 cgatgtcgca catcgcggac aatcgcggcg gcggtgatcg cgccgccgcg aatccgcgca
    21601 atactcaggc ggccggttcg gcgctatgcg actctttatt ctgcgcaatc caagtcagtg
    21661 cggattcgac gtcggcaagc tcgctggcag tgaacccctc gattctgacg ccggtcgctt
    21721 ccgccttctt cttggcgagt tcggcggccg caaccgtgtg cacccaaacc aacccgcggc
    21781 gtactttggc ggtgaatttc tgctgctttt cttcggtcat agttcgtctc ctttatattt
    21841 tccagacata ggtcgggcac gatacacgac taaggccgcc gaaaggaacc cgcactatcc
    21901 aattgcgcac tgtaggcgtt gagcgcgcgc gcgaccaccc ctatgtattg gtcggcctcc
    21961 tcccagcggc gctcttcgat ggcctcacga attccgggca gtgtctttac cgcgtacccg
    22021 gtatgcgctc ccggcgcata gaccatgtgc tgataccatg cgcggcccgg caagcccttt
    22081 gaatcggtca aggactgctc gagtcgcgtc agtatcgcgt tcagctgctc gcgttcgcca
    22141 gcggcttgtt gagcatcgcc ggcctcgatg cgcgcgtatt cttggtcgaa tgcctgcgcg
    22201 ctctgctcga gcttcgcgat ggcattgtcg agctcggcga aattgagata gggaacctcg
    22261 gcgttgcgcg gcggcgcggc gcgcggcttc gcagggtccg cggccaacgc gtaggcgccg
    22321 gcgtcgagca gcttcgataa ctcggcggtc cgcacgcgca tgccgtcggc cagtttgtgc
    22381 agctcctcat cataggcggc aatcgaggcc gcaaaatcgc cttctcgcgc gggcaataat
    22441 tgcgcctgcg acgcgcgcag cgtcatgcgt cccgcaacct tggcgagcgc cacgccgtat
    22501 tcgaaggtgg gatcgacgaa acgccgaaag tgctcgaacg tatcgtaggc cgagtgatac
    22561 acaccgtatt cggtttcgcc ctggaaactt acgttcaggg aactgacgcc caggtgctcg
    22621 aggaaggggc tgaagtcgga gccggcgccg agcgcgccga gttgtaggtc gccgctcggg
    22681 ttcgcacgag cgcccgactc gtaactgacg gtgtgctccc gggcgagcgc ccgcgccaga
    22741 acgctcgcgc cggtttcggg gtccgctaca tcgcgcgcca cttcgctggc aaaatgctgc
    22801 aaggcgtggc cgcccgccac ctctagatag ccccggccgt tcgtgtccga attgatatac
    22861 aacaccgctt tggctttgag ttcggcgacg tgcaattcag tccactcggt cgaaccgagg
    22921 agacccggct cttcgccgtc ccagctcgcg tagatcaggg tgcggcgcgg ccgccatccg
    22981 ctcttcacca atttgccgat cgcctggg
//
LOCUS       DASWZT010000062         2858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_82749, whole genome shotgun sequence.
ACCESSION   DASWZT010000062 DASWZT010000000
VERSION     DASWZT010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82749"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VGE92_01695"
     CDS             <1..473
                     /locus_tag="VGE92_01695"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY1312554.1"
                     /translation="APWTRAAEDPQPLAYLDAPSIREGGAIDVQLEVSIETEKMRRAG
                     TPPARLSRSNFKDSYWNIAQLVTHHTVNGCNLRPGDLLGTGTQSGPSPAEAGSLLELS
                     QGGKCAVPLIDGETRRFLEDGDAVTLRGWCERPGAARIGFGAATGRVLASQPFN"
     gene            complement(466..1755)
                     /gene="hmgA"
                     /locus_tag="VGE92_01700"
     CDS             complement(466..1755)
                     /gene="hmgA"
                     /locus_tag="VGE92_01700"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEY1312555.1"
                     /translation="MNYLSGFGNHFASESVRGALPVGRNSPQRTPFGLYAEQLSGTAF
                     TAPRAENRRTWLYRLRPSAAHPPFALIANQGLLRSGPFVEAGPTPNRLRWNPAPLPAG
                     SIDFLDGLVTYAGNGDPAQGVGMAVHGFAANRSMIDRVFFDADGELLIVPQHGRLRFV
                     TEMGVLEVEPHQVVLIPRGVRFRVELIDAGARGYVCENYGAPFRLPELGPLGSNGLAN
                     ARDFESPAAHFEDLETRTVVVQKFAGKLWSTELDHSPLDVVAWHGNLAPCRYDLRRFN
                     AVGSISFDHPDPSIFTVLTSPSDTPGTANCDFVIFPPRWLVAENTFRPPWFHRNVMSE
                     FMGLISGRYDAKAGGFEPGGASLHNQMSAHGPDLTSYRDAIEAPLVPQHIEDTMAFML
                     ESRCIIRPTRFALETPLLQPDYDRCWQGFKKAACPAS"
     gene            complement(1752..2819)
                     /gene="hppD"
                     /locus_tag="VGE92_01705"
     CDS             complement(1752..2819)
                     /gene="hppD"
                     /locus_tag="VGE92_01705"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEY1312556.1"
                     /translation="MEDPRDNPLGLDGFEFVEFTGPDPAALAALFTAMGFTHVASHRS
                     KNVRRYAQGDINFLLNMDAGGQVADFRAKHGPSANAMAFRVKDARLAFEEALRRGAKA
                     VTGPVGPMELNIPAIEGIGGSNLYLVDRYGAHEIYDVDFTPTPGAARDQHSTGLHTLD
                     HLTHNVNRGRMSHWATFYQRIFNFREIRYFDIEGQATGLFSKAMTAPDNKIRIPLNES
                     QDEHSQIEEFLKQYKGEGIQHLALATDDIFATVDKLRANGIRFQDTPESYFDLIDQRL
                     PGHGHDVAEMRKRRVLIDGAPETGGGILLQIFTENMVGPIFFEIIQRKGNEGFGEGNF
                     KALFESLELDQIRRGVVKVTP"
BASE COUNT          537 a          871 c          908 g          542 t
ORIGIN      
        1 gggctccctg gacgcgcgcg gccgaggatc cgcagccgct tgcctacttg gatgcgccga
       61 gcattcgcga aggcggcgcc attgacgtgc aattggaagt gtcgatcgaa accgagaaga
      121 tgcgccgcgc cggcacgccg cccgcacgct tgtcgcgcag caacttcaaa gactcatact
      181 ggaacattgc gcagctcgta actcaccaca ccgtcaatgg ctgcaatttg cggcccggtg
      241 atctgctcgg gaccggaacc caatccgggc cctcgcccgc agaggccggt tcactgctgg
      301 agctgtcgca aggcggcaag tgcgcggttc cattgatcga cggcgagact cgccgcttcc
      361 tcgaggacgg cgatgcggtc acgctgcgcg gttggtgcga gcgcccgggg gccgcgcgta
      421 ttgggttcgg cgcagccacc ggacgcgtcc tcgcatcgca accattcaac tagcggggca
      481 ggcggccttt ttaaatccct gccaacagcg atcgtagtcc ggctgcaaga gcggggtctc
      541 gagcgcaaag cgggtgggcc gaatgatgca tcggctttcc aacatgaacg ccatcgtgtc
      601 ctcgatgtgc tgcggcacca gaggggcttc gatcgcgtcc cggtagctgg tcaggtcggg
      661 cccatgcgcg gacatttgat tgtgcagcga ggcgcctccg ggttcgaagc cgccggcctt
      721 ggcgtcgtag cggccgctta tcagacccat gaactcgctc atcacgtttc gatgaaacca
      781 cggcggccga aacgtgttct ccgcgaccag ccaacgcggc gggaagatca cgaagtcgca
      841 gttcgccgtt ccgggcgtgt cgctcgggga ggtgagcacg gtaaaaatcg acggatcggg
      901 atgatcgaag ctgatcgagc ccacggcatt gaagcggcgc aggtcgtagc ggcagggcgc
      961 gaggtttcca tgccacgcga cgacgtcgag tgggctgtga tcgagctcgg tgctccacaa
     1021 tttacccgcg aacttttgca cgaccaccgt gcgtgtctcg agatcttcga agtgcgccgc
     1081 gggcgattcg aaatcacggg cattggccag gccgttcgaa cccaagggac cgagctccgg
     1141 caatcgaaac ggtgcaccgt aattttcaca cacatagccg cgcgcgccgg cgtcgatcag
     1201 ctcgacgcga aagcgcacgc cgcgcgggat caagacgact tgatggggtt cgacctcgag
     1261 cacgcccatc tccgtgacga accgcagcct tccgtgctgc ggaacgatca gcagttcgcc
     1321 atcggcatcg aagaagacgc ggtcgatcat cgaccgattc gcggcaaacc cgtgtacggc
     1381 cattccgaca ccctgcgcag gatcgccgtt gccggcatag gttaccaacc cgtcgagaaa
     1441 atcgatcgat cctgccggca gcggcgccgg gttccagcgc aaacgattcg gcgtgggccc
     1501 ggcctcgaca aacggacccg atcgcaacag tccttggttc gcgatgaggg cgaatggcgg
     1561 atgtgccgcg gacgggcgca gccgatacag ccaagtccgc cgattctccg cgcgcggtgc
     1621 cgtgaacgcc gtgccggaca gttgctcggc gtagagcccg aacggcgtgc gctgcggaga
     1681 attgcgcccg accggcagcg cgccgcgaac gctctcgctg gcaaaatgat tgccgaagcc
     1741 gctcaaatag ttcacggcgt gactttgacg accccgcggc ggatctgatc gagttccagg
     1801 ctctcgaaca gtgctttgaa attgccttcg ccgaaccctt cgttgccctt gcgctggatg
     1861 atctcgaaga agatgggccc gaccatgttc tcggtgaaga tttgcagcag gatgccgccg
     1921 ccggtttccg gcgcgccatc gatgagcacg cggcgcttgc gcatttcggc gacgtcgtgg
     1981 ccgtggccgg gcaggcgctg gtcgatcaaa tcgaaatagg actccggcgt gtcttggaag
     2041 cgaattccgt tggctcgcaa tttgtccacc gtagcgaaga tgtcgtccgt tgccagtgcc
     2101 aggtgctgaa tgccctcgcc cttgtattgc ttcaagaact cttcgatctg gctgtgttcg
     2161 tcctggcttt cgttcagggg aatgcgaatc ttgttatcgg gagccgtcat cgccttggaa
     2221 aataagccgg tggcctgacc ttcgatgtcg aagtagcgga tctcgcgaaa attgaaaatc
     2281 cgttgataga aggtcgccca atgggacatg cgcccacggt tcacgttgtg ggtgagatga
     2341 tcaagggtat gcaagccggt gctgtgctgg tccctggcgg caccaggagt cggcgtgaaa
     2401 tccacatcgt aaatttcgtg cgccccgtag cggtctacca ggtacagatt cgagccgccg
     2461 atgccttcga ttgccggaat gttcagttcc atcgggccca cgggtcccgt cacggctttg
     2521 gcgccgcgcc gcaaggcctc ttcgaacgcg agtctcgcgt ccttgacgcg aaatgccatg
     2581 gcgttcgccg aagggccatg cttggcgcga aagtccgcta cctgcccgcc ggcatccatg
     2641 ttgagcagga agttgatgtc tccttgagcg tagcggcgca cgttcttgga gcgatgactc
     2701 gcgacgtggg tgaagcccat ggcggtgaat agcgcggcga gagccgcggg atccggcccg
     2761 gtgaactcga cgaattcaaa accgtccagt ccgagcggat tgtcgcgagg atcttccatt
     2821 gccgcctccc gaagtggtat catttgttac tatatacc
//
LOCUS       DASWZT010000063         2534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_68234, whole genome shotgun sequence.
ACCESSION   DASWZT010000063 DASWZT010000000
VERSION     DASWZT010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68234"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="VGE92_01710"
     CDS             complement(<1..969)
                     /locus_tag="VGE92_01710"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HEY1312557.1"
                     /translation="MRALFVGLIVACCGNLLPGAAIAADAQFDIRILSDTLADPSPDA
                     LAKGALDDQFAPLNGKLRSRGKAFWLRLMPTLRTADSLTGIPVVVMHSGRQTEADLYA
                     ARGGAAVQLSQVTHLPEFRGTQDTVFSFVDGANPGQPLYVRINVAGNDSETVSFAAST
                     LDRVLRRGAEHARVIALTFGALMAVSLAALLIWFVLADRLFILYATLFSLQALYVTYL
                     SGQGFDWPWLSFAQPLGSFAWNVPVGLSGAIACLFTREIADLRHFSPRIYTAFGWFAV
                     AFVGITAANLAKFIGIGSWVNALGNLLFVILSLFTMVVAFVAWRRGN"
     gene            complement(984..1928)
                     /locus_tag="VGE92_01715"
     CDS             complement(984..1928)
                     /locus_tag="VGE92_01715"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEY1312558.1"
                     /translation="MNDAADRRVRSEDESASADGFGAQLEAWQARMEQALAARLPDSR
                     MVPARLHEAMRYSVLGGGKRIRPALLFATARTLGLSEDEVEAAACAIELVHVYSLVHD
                     DLPAMDNDDLRRGRPTCHKAFDEATALLVGDALQPLAFQLLANDPALPESPAIRLRLI
                     DVLAQAIGTFGMAGGQAIDLAVQGKQLDIGQVEDMHARKTGALIRASVLMAAECVPPL
                     DANLYAALARYASALGLAFQIQDDLLDVTGDVSTLGKATGADSERSKPTHPAIIGIQA
                     SQQRVRLLHSQAIHALAPFGDKAESMRSLAHWLLSRQY"
     gene            complement(1957..>2534)
                     /locus_tag="VGE92_01720"
     CDS             complement(1957..>2534)
                     /locus_tag="VGE92_01720"
                     /inference="COORDINATES: protein motif:HMM:NF023372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3463 domain-containing protein"
                     /protein_id="HEY1312559.1"
                     /translation="ATQLGVEGITVSPGYSYHHAPKQDVFLARNASKQLFRGIFSRRK
                     RSSGISWSFNHSALFLDFLAGNQTYQCTPWSNPTYNVFGWQRPCYLLSDEGYAPSYRS
                     LIEDTEWEKYGVGKNPRCDNCMAHCGFEGTAVNDAFSHPLKALKVWMRGPKISGAMAP
                     DLPVNYTDAARYGTVASIPLSDIKRASRESV"
BASE COUNT          515 a          801 c          795 g          423 t
ORIGIN      
        1 attgccacgc cgccaggcga cgaacgccac caccattgtg aacagcgaca atatgacgaa
       61 caagagattg ccgagcgcgt tcacccagga accgatcccg atgaacttcg cgagattcgc
      121 ggcggtaata ccgacgaagg cgacggcgaa ccagccgaag gcggtgtaga tccgcggcga
      181 gaagtggcgc aggtccgcga tctcgcgcgt gaataggcag gcgatcgcgc cgctcaatcc
      241 gaccggtacg ttccaagcga aggaacccaa tggttgcgcg aagctaagcc aaggccagtc
      301 gaagccctga ccggagagat atgtcacata caacgcctgc agggagaaca gtgtcgcgta
      361 caaaatgaac agtcgatccg ccagcacgaa ccaaatcaac aaggcggcaa gcgacaccgc
      421 catcaatgcg ccgaatgtca gcgcgatcac tcgcgcatgc tcggcgccgc gcctcagcac
      481 acgatcgagc gtcgacgccg caaagctcac cgtctccgag tcgttgccgg cgacgttgat
      541 acgcacgtac agcggctgcc ctggattcgc cccgtcgaca aaactgaaca cggtgtcttg
      601 agtaccgcga aattcgggga ggtgcgtgac ttgcgagagc tgcacggccg cgccgccgcg
      661 cgccgcatac aggtcggctt cggtttgccg tccggagtgc atcacgacga cggggatccc
      721 ggtcagcgaa tcggctgtgc gcagcgtagg cataagccgc aaccaaaacg ctttgccgcg
      781 gctgcgtagt ttgccgttga gcggcgcaaa ttggtcgtcg agcgcgccct tcgcgagggc
      841 atcgggcgag gggtcggcga gcgtgtcgct gagaatccgg atgtcgaact gcgcgtcagc
      901 ggcaatcgcc gcccccggaa gcagattccc gcagcaagcg acgatcaacc caacgaacag
      961 cgcacgcacg gcgtgtgtgg gtatcaatac tgccgtgaca acagccagtg agccaaggaa
     1021 cgcatcgatt ccgccttatc gccgaacggc gccagcgcat gaatcgcctg actgtgaagc
     1081 agacggaccc gttgctgcga ggcctggatg ccgatgatgg ccggatgggt gggcttcgag
     1141 cgttcgctgt cggctccggt ggccttaccc aaggtcgaca catcacccgt gacgtcgagc
     1201 agatcgtcct gaatctgaaa cgccaaaccc agcgccgagg catatcgagc cagagccgcg
     1261 tagaggttcg catcgagcgg cggcacgcac tcggccgcca tcaatacgct ggcgcgaatc
     1321 agggctccgg tcttgcgtgc atgcatatct tccacttggc cgatatcgag ctgcttgccc
     1381 tgaaccgcca agtcgatggc ctgaccgccg gccatgccga aggtgcctat tgcctgggcc
     1441 aggacatcga tcagccgcag tcgtatcgcg ggagattccg gcaacgccgg atcgttggcg
     1501 agcaactgaa acgcgagcgg ctgcagagca tcgcccacca acaatgccgt ggcctcatca
     1561 aaggccttgt ggcaggtcgg ccggccgcgc cgcagatcgt cgttatccat ggcgggcaaa
     1621 tcgtcgtgta ccaacgagta gacgtgcacg agttcgatag cgcatgccgc cgcctcgact
     1681 tcgtcttcgg aaagacccaa ggtgcgcgcc gtggcgaaca acagggccgg gcgaatgcgc
     1741 ttgccgccgc ccagaacgct gtagcgcatc gcttcgtgca gacgcgcggg caccatccgg
     1801 gagtccggca ggcgcgccgc cagcgcctgt tccatgcgcg cttgccaggc ctcgagttgc
     1861 gctccgaatc catcggcgct tgcggactcg tcctccgacc tgacgcgacg atcagccgcg
     1921 tcattcacgc gtgtaagccg tcgtcagtct gacgcctcac acgctctccc ggctcgcgcg
     1981 cttgatgtca ctgagcggga tcgacgccac cgtgccgtag cgcgcagcgt ccgtatagtt
     2041 gacgggtaaa tccggcgcca tggcgccgct gatcttcggt ccgcgcatcc aaaccttgag
     2101 cgctttgagc ggatggctga acgcgtcgtt gacggccgtg ccttcgaacc cgcaatgcgc
     2161 catgcagttg tcgcagcgcg ggttcttgcc gacgccgtat ttctcccatt cggtgtcctc
     2221 gatcagcgaa cgatagctcg gcgcatagcc ctcatcggac agcaagtagc aaggccgctg
     2281 ccacccgaac acgttgtagg tggggttgct ccacggcgtg cactgatagg tttggttgcc
     2341 ggcgaggaaa tcgaggaaca gcgcggaatg attgaaggac caggaaatgc ccgacgaacg
     2401 cttgcggcgg ctgaagatgc cgcggaacaa ttgcttgctg gcattgcgcg ccagaaagac
     2461 atcctgtttg ggagcgtggt ggtagctgta ccccggcgag acggtgattc cttcgactcc
     2521 gagctgcgtc gcca
//
LOCUS       DASWZT010000064         4669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_10247, whole genome shotgun sequence.
ACCESSION   DASWZT010000064 DASWZT010000000
VERSION     DASWZT010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10247"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1631)
                     /locus_tag="VGE92_01725"
     CDS             complement(<1..1631)
                     /locus_tag="VGE92_01725"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1312560.1"
                     /translation="MSRRHRAGANRPHTVGSSRRPLVEEAFRLHQAGRLEQAKALYLK
                     ILAVKSDDADALHLLGVVHHQQGRPSEAVELVTRAIAIKPNYGEAYSNLGVSLAALGR
                     LDAAVAAYRHAIVIKPDIAHWHRNLGGALAAQGKFEEAGECFRQAIAISPAYAEAHAD
                     LATSLKELRRFDEAAESLRQAVALMPTVADWHSNLGMALTTLGRFDEALTCLRQAIAI
                     RPDYAAAHHNMAIAFKELRRFDEAAASLRRAIALMPNIPDWHCHLGMALTALGQWDEA
                     VVCLRQAIALQPDNPLACDRLAFALKYLCQWDEAVACLRQAIALRPDYAEAHGHLGML
                     LLLLGHLREGFDEYRWRWHAANFPDKPPELPCPQWEGENLAGKTILVCCEQGYGDSLQ
                     FIRYVIPLSRIAGRVWVQTDLSLVSLFRSIAGIEVVTEAPAASHSFDYHVPLLCLPRL
                     FATELETIPAEVPYLSPAASKIDHWAQRLAPGDGKIKIGLVWAGNAGIEDVDANAMDL
                     RRSVRLKQFAPLADIANVRFFSLQKGEPSEQASHPP"
     gene            complement(1648..3075)
                     /locus_tag="VGE92_01730"
     CDS             complement(1648..3075)
                     /locus_tag="VGE92_01730"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY1312561.1"
                     /translation="MKQRWLRGSLIGVGAVGLCTAGAMAFATPPDPFGTDAALHRDTA
                     GYNDPLGHDCAIPGGELTFTAAVDLALCRNPQTRASWAAAHQQAAALGSAESAWLPSV
                     TVNAGASRDIGQHVDVTGNILNNAQNTGDAAATLSWTLYDFGARSGRIKSARSLMDAA
                     AATAGSVAQQVVRLVVQSYYGVVAGDADLAAAKITDDVTAHSVEIARSLREGGVGTLA
                     DVLQAETAHGQAVLARVQAAAAAKSARGTLAVTLGLSADQAFKLAAEPVPTEVPALIA
                     RMADLMAEAARQRPDLKAAQAQRDAAEANITVARALGRPSILLGAQHSFNYQTGVPNE
                     NYNQVGISLTWPLFTGFNVSYGVRQAQAALQQQDANLEQVGLTVTLDVWTSYYSLDSA
                     NQALTITAGLTKTAEDNLQVALGRYQAGVGTIVDVLTAQSAAATARTLRINAELGWKV
                     ARANLALALGRLSSAQPLQNGAALP"
     gene            complement(3164..>4669)
                     /locus_tag="VGE92_01735"
     CDS             complement(3164..>4669)
                     /locus_tag="VGE92_01735"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1312562.1"
                     /translation="AADQGFIAAYERLGALYAEGLGLPQDFQAAADWFQRAAALGDAN
                     ALYHLGTLQMGGLGTPRDPRAARQWYRKAAQKGSGTACLRLGVICATGLDVNQNYQVA
                     ARWYAQAAVHGVPEGRYNLAFLHFRGLGVPQDTGRAIKLLEHAADAGSLQAAWALYHQ
                     YKDNTYVPRDAAAAARWLLRAADMGSAAAAGLLFQMLNDKDPLAPPPERVGALLEKSA
                     AHGDPGAQASLALWYLEGKHAPRDEARAVRWFERAAHGGNVFAQAWLGDALVTGQILK
                     ADPQAAAEWYERAALQGHAGATQALTTLRVAAGAGPEDMARLFKHWLTGAKRGDAVAQ
                     RIVGDFYLKGIGVETSLEEARRWLGAAAEQGNAPAMVLLAGSILQESGQWQHFPQAVE
                     WFRRAAAQGNVDAEYNLGVCLRRGLGVAANDDAAEQHYRSAAQRNHVSAQLALGDLIA
                     GRASNDADWNEAARWYRLAADGGHESGKERLIQLNARRGVNPPETSATQPA"
BASE COUNT          793 a         1571 c         1471 g          834 t
ORIGIN      
        1 ggcgggtgcg atgcttgctc cgacggctcg cccttctgca agctgaagaa tcgcacattt
       61 gcaatgtcgg cgagcggcgc gaattgtttc aatctcacac tccggcgaag atccatcgcg
      121 ttcgcgtcga catcctctat gcccgcgttg cccgcccaga ccaagccgat tttgatcttg
      181 ccatcccccg gcgccaggcg ttgagcccag tgatcgatct tcgacgcggc gggtgacagg
      241 tagggaacct cggctggtat cgtttcaagc tctgtcgcaa acaatcgcgg caggcacagc
      301 agcggtacgt gatagtcgaa gctgtgagac gcggccggag cttcagtcac cacttcaatg
      361 cccgcaatgg atcgaaacaa gctcaccagc gaaagatcgg tctgaaccca cacgcgcccg
      421 gcaatcctcg ataacggtat aacgtaccga ataaattgta ggctatcgcc gtacccttgc
      481 tcacaacaga cgagaatggt ttttcctgcg agattttcgc cttcccactg cgggcatggc
      541 aattccggcg gtttatccgg aaaattcgcg gcatgccaac gccatcgata ctcgtcgaac
      601 ccctcgcgca agtgacccag caacagcaac agcatgccga ggtggccgtg cgcctcggcg
      661 tagtcgggtc gcagggcgat tgcttgacgc aaacaagcga ccgcctcgtc ccactggcaa
      721 agatacttga gagcaaacgc caggcggtcg catgccagcg ggttgtctgg ttgcagggct
      781 atggcctggc gcagacaaac gaccgcctcg tcccactggc cgagcgcggt caacgccatg
      841 ccgagatggc aatgccaatc ggggatgttc ggcatgagcg caattgctcg gcgcaaagag
      901 gcggcggcct cgtcgaaccg gcgcagctcc ttgaatgcga tcgccatgtt atggtgtgcc
      961 gccgcgtagt cgggtcggat tgcaatcgcc tgacgcaggc aggtaagagc ctcgtcgaat
     1021 cgaccgagcg tggtcaacgc catgccgaga ttgctgtgcc agtcggcgac ggtcggcatg
     1081 agcgccaccg cttgacgcaa actttcggca gcctcatcga agcggcgaag ctccttgagt
     1141 gaagtcgcca aatcggcatg tgcctccgca tacgcggggc tgatggcgat cgcttgacgg
     1201 aagcactcgc cggcctcctc gaatttcccc tgcgccgcga gcgccccgcc gaggttgcgg
     1261 tgccagtgtg cgatgtccgg cttgatgacg atggcgtggc gataggcggc tacggcggcg
     1321 tcgagccggc cgagggctgc caagctcacg cccaaattgc tgtacgcctc cccgtagttc
     1381 ggcttgatcg cgatggcgcg ggtcaccagc tcgaccgcct ccgaaggtcg accctgctga
     1441 tggtgcacca caccgagcag atgcagtgca tcggcatcat cagacttcac cgccaagatt
     1501 ttaagataca gcgctttcgc ctgttccaag cggccggctt gatgcaggcg gaatgcttct
     1561 tcgaccaacg gccgacgcga cgaccccacg gtgtgaggtc gattggcgcc cgcccgatgc
     1621 cgccggctca ttagcgcgct cgcggcgtca cggcagcgcc gctccgttct gcaggggctg
     1681 tgcgctggaa aggcgcccga gcgccagcgc gaggttcgcg cgcgcgactt tccagccgag
     1741 ctcggcgtta attcttaagg ttcgcgccgt cgccgccgcg ctctgcgcgg tcaacacatc
     1801 gacgatcgtg cccacccccg cttgatagcg gccgagcgcg acttgcagat tatcctcggc
     1861 ggtcttggtg aggcccgcgg tgatggtcag cgcctgattg gcggaatcga gcgagtagta
     1921 gctggtccac acatcgaggg tgacggtcaa acccacctgc tccaagttgg cgtcttgctg
     1981 ctggagcgcg gcctgcgcct ggcgaacgcc atacgacaca ttgaatccgg tgaagagcgg
     2041 ccaggtgagg ctgatcccga cctgattgta gttttcattc ggtacgcccg tctgataatt
     2101 gaaggagtgc tgcgcgccga gcaatatgga cgggcggccg agtgcgcgcg ccaccgtgat
     2161 gttcgcctcg gcggcgtcgc gctgcgcctg cgcggcctta agatcgggcc gctggcgcgc
     2221 ggcttccgcc atcagatcgg ccatgcgcgc gatgagcgcc ggcacctccg tgggcacggg
     2281 ttcggcggcg agcttgaacg cctggtcggc ggagaggccg agcgtcaccg ccagcgtgcc
     2341 gcgggcgctc ttggcggccg ccgcggcttg cacgcgcgcg agcaccgctt ggccatgcgc
     2401 cgtttcggcc tgcagtacat cggcgagcgt gcccacaccg ccctcgcgca gcgagcgggc
     2461 gatctcgacg ctgtgcgccg tgacgtcatc cgtgatcttg gccgccgcga gatcggcatc
     2521 gccggcgacg acgccgtagt aactctgcac caccaagcgc accacctgct gggccacgct
     2581 gcccgcggtc gctgctgcgg cgtccatcag gctgcgggcg ctcttgatgc gtccgctccg
     2641 ggcaccgaag tcatacaggg tccaactcaa cgtggctgcg gcatcgcctg tgttttgcgc
     2701 gttgttcaag atgttgccgg tcacatcgac gtgttgcccg atatcccggg aggcaccggc
     2761 gttcaccgtc accgagggga gccatgcgct ttcggcgctg ccgagcgctg ccgcttgttg
     2821 gtgcgcggcg gcccaagagg cccgtgtctg gggattgcgg cacaacgcga gatcgaccgc
     2881 tgcggtaaag gtcagctcgc cgccgggaat ggcgcagtcg tgacccagag gatcgttgta
     2941 cccggcggtg tcccggtgca acgccgcgtc ggtaccaaag ggatcgggcg gcgtcgcgaa
     3001 agccattgcg ccggccgtgc aaagtcccac ggcgcccact ccgatgagac tacctcgcag
     3061 ccatcgttgc ttcatcccca ctctgctttt ttgcaggcaa attgcgtctt ggttttcgct
     3121 atcgagcatg atttttcggc attcgcggcg gctcccgcga actctacgcg ggttgcgtcg
     3181 cggaggtctc gggcgggttc acaccgcggc gcgcgttaag ttgaatcaag cgctccttgc
     3241 ccgattcatg gccgccatcc gcggccaatc ggtaccagcg cgcggcttca ttccaatccg
     3301 catcgttgga agcccgtccg gcgatgagat cgccgagcgc gagctgtgcc gagacgtggt
     3361 tgcgctgagc cgccgaccga tagtgctgtt cggcggcgtc atcgttcgcc gcgacgccaa
     3421 gtccgcgccg caggcacacg cccaagttgt attccgcgtc cacattgcct tgtgcagctg
     3481 cgcgccgaaa ccactcgacc gcctgcggaa agtgctgcca ctggccgctc tcctgcaaaa
     3541 tcgaacccgc caacagcacc atcgcggggg cattgccctg ctcagcggcg gcgcccaacc
     3601 agcgccgagc ctcctcaagt gaggtttcca cgccgatccc tttgagataa aaatcaccca
     3661 caatgcgctg tgccacggca tcgccgcgct tggcgccggt cagccagtgc ttgaagagcc
     3721 gcgccatgtc ctcgggcccc gcgcccgcgg cgacacgcag cgtcgtcaat gcctgcgtgg
     3781 ctccggcgtg cccctgcagc gccgcgcgct cgtaccactc ggcggccgct tgcggatccg
     3841 ccttgaggat ttgaccggtc acgagcgcat cgccgagcca tgcctgcgca aagacattgc
     3901 cgccgtgcgc cgcgcgctcg aaccagcgca ccgcccgcgc ctcgtcgcgc ggcgcgtgct
     3961 tgccttcgag ataccacagc gcaaggcttg cctgggcgcc cggatcgcca tgcgcggcgc
     4021 tcttctccaa caaagcgccg acccgctcgg gcggcggtgc caagggatct ttgtcgttca
     4081 gcatctgaaa cagcaatccg gcggccgcgg cactgcccat gtccgcggcg cgcaacagcc
     4141 agcgcgccgc cgctgccgcg tcgcgcggca cgtaggtgtt gtctttgtac tgatgataga
     4201 gcgcccatgc ggcttgcagg ctgccggcgt cagccgcgtg ttccaggagc ttgatggccc
     4261 tgccggtgtc ctgcggaacg cccaaaccgc gaaaatgcaa aaacgcgaga ttgtagcggc
     4321 cttcgggcac accatgcacc gcggcctgcg cataccagcg cgccgcgact tggtagtttt
     4381 gattcacatc gagacccgtt gcgcaaatga cgccgagacg cagacacgcg gtaccgctgc
     4441 ccttctgcgc ggccttccgg taccactggc gcgctgcccg cgggtcgcgc ggcgtgccga
     4501 ggccgcccat ttgcagcgtg cccaagtgat acagcgcgtt ggcatcgccc aacgcggccg
     4561 cacgctgaaa ccagtcggcc gccgcctgga aatcctgcgg caatcctaag ccttccgcat
     4621 acagcgcgcc caagcgttca taagccgcaa tgaagccttg atccgcggc
//
LOCUS       DASWZT010000065         2342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_550, whole genome shotgun sequence.
ACCESSION   DASWZT010000065 DASWZT010000000
VERSION     DASWZT010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VGE92_01740"
     CDS             complement(<1..182)
                     /locus_tag="VGE92_01740"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HEY1312563.1"
                     /translation="MNTTAENDPKSGFPTANTPPIVSAQEWEAARVELLAKEKAFTRA
                     RDALAAERRRMPWLAV"
     gene            273..1493
                     /locus_tag="VGE92_01745"
     CDS             273..1493
                     /locus_tag="VGE92_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312564.1"
                     /translation="MDSLITAAARALAAGDPLGALKRVALRDDAAALALRGIAMAQLG
                     DLARAKALLRRAARAFGPKEAVARAKCVIAEAEIALASRDLLWAAKGLQAARATLEAH
                     GDRVNAAHASYLEVRRLLLIGRLEEAERTLAQLDPEPLPAASRAVHELMFAGIAMRRL
                     RAKAAQAALARAEIAARRAAIPALSAEVDSASRVLGTPVARLTGNGGERLLLLEEVEA
                     LLASEALVVDASRHVLRQERQVVSLATRPVLFAIARMLGEAWPNDVPRDALIARAFRL
                     KVADESHRARLRVEVARLRVVLRKVADVNATKRGFALIPRRARQVVVLARLVEEKHAG
                     VLAFLADGESWSSSALTLALGASQRTVQRALESLAAAGKVQSFGHGRTRRWMTPPTPG
                     FATTLLLPAPLPID"
     gene            1503..2147
                     /locus_tag="VGE92_01750"
     CDS             1503..2147
                     /locus_tag="VGE92_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312565.1"
                     /translation="MSKSTAAEIVREYGPFLGAERVDGVTYDGERIWFASGDKLNAID
                     PASGKLLRSIDVAAHAGTAFDGEYLYQLAEKHIQKLDPESGRVIATIPAPGGGADSGL
                     TWAEGTLWVGQYRDRKIHQIDPETGAILRTIESNRFVTGVTWVDGELWHGTWEGDDSD
                     LRRIDPRTEKVLERLEMPPGTGISGLESDGGDLFFCGGGKSGKVRTVRRPKKSG"
     gene            complement(2144..>2342)
                     /locus_tag="VGE92_01755"
     CDS             complement(2144..>2342)
                     /locus_tag="VGE92_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312566.1"
                     /translation="YGIGLIILMSLVFGTTTIATYVLLCVYSTAGLRRVSLGPLERYG
                     EVLSGGFIALVGVAFWLWPVI"
BASE COUNT          411 a          739 c          786 g          406 t
ORIGIN      
        1 tctaccgcaa gccacggcat ccggcgacgc tcagccgcca gcgcgtcacg cgcacgggtg
       61 aaggccttct ccttcgccag cagctctaca cgggccgcct cccactcctg cgccgacacg
      121 atcggcggtg tattggccgt cggaaatccg cttttggggt cattctcggc tgttgtattc
      181 atggtcgttg aacctcgtct cgttcgttgc tcgtgagata gttatagccc cggacaccga
      241 ctggtgggag ttacaaatgt gacgggattg agatggactc gctgatcacc gccgcagcac
      301 gcgcactcgc cgcgggcgat cccttgggtg cattgaaaag ggtcgccctg cgggatgacg
      361 cagcggcgct cgcgctacgc ggcattgcca tggcgcagct cggagacctc gcccgcgcca
      421 aggcactctt gcggcgggct gcgcgcgcct ttggtccgaa agaagccgtg gcccgagcga
      481 agtgcgtcat cgcagaggcc gagatcgcac tcgcctcgcg cgacttgctc tgggcggcaa
      541 agggtctgca ggcggcgcgg gcaacgctcg aggcgcacgg cgaccgggtg aatgccgcgc
      601 atgcgtccta tctcgaggtc cggcgcctgc ttctgatcgg gcgcctggaa gaggccgagc
      661 gtacgctcgc ccagctcgac ccagagccgc ttccggccgc atcgagggcc gtgcacgagc
      721 tcatgttcgc gggaatcgca atgcgtcgtc tgcgggcgaa ggcggcgcag gccgcgctcg
      781 ctcgggccga aatcgcggcg cggcgcgccg cgatcccggc actcagcgcg gaggtcgata
      841 gcgcatcgcg ggtgctcggc acgcccgtcg cgcgcctgac tggcaatggc ggcgagcgac
      901 ttctcttgct cgaggaggtc gaagcactgc tggcatcgga ggcgttggtg gtggacgcgt
      961 cccgtcatgt cctgcgtcag gaacgccaag tggtgtcgct cgccacccgt ccggtgttgt
     1021 tcgcgatcgc acgcatgttg ggcgaagcgt ggccgaacga tgttccgagg gacgcgctca
     1081 tcgcgcgcgc cttccgattg aaggtggcgg atgaatcgca tcgcgcgcgc ttgcgagtcg
     1141 aagtcgcgcg gcttcgcgtg gtgcttcgca aagttgccga cgtgaacgcg accaagcgcg
     1201 gctttgcgct catcccgcga cgcgcgcgcc aagtcgtcgt gctcgcacgc ctcgtcgagg
     1261 agaaacacgc gggcgtgctc gcgtttctcg ccgacggtga atcgtggtcg agctcggctt
     1321 tgaccctcgc gctcggagcc agccagcgca ccgtccaacg ggcgctcgaa tcgctcgcgg
     1381 cggccggcaa ggtgcagtcg ttcgggcatg ggcgaacgcg gcgctggatg actccgccga
     1441 cgcccggatt cgcgacgact ttgttactcc ccgcgcctct gccgattgac taagataccc
     1501 ccatgagtaa gtcaaccgcc gccgagatcg ttcgagaata tggccccttt ttgggtgccg
     1561 agcgcgtgga tggagtcact tatgatggcg agaggatttg gtttgcctcc ggcgacaaac
     1621 tgaacgcaat cgatccagcg agcggcaagc tgctgcgctc gatcgatgtg gccgcgcatg
     1681 ccggaacggc ctttgacggc gaatatctgt atcagctcgc ggagaaacac atccagaagc
     1741 tcgatccgga gagcgggcgc gtcatcgcca cgatcccggc gccggggggc ggcgctgact
     1801 cgggcctcac gtgggccgaa ggcacgctgt gggtcggaca atatcgagat cgcaaaatcc
     1861 atcaaatcga tcccgaaacg ggggcgattc ttcgcacgat cgaatccaac cgtttcgtta
     1921 ccggggtcac ctgggtcgat ggcgagctgt ggcacggcac ctgggaaggc gatgacagcg
     1981 atttgcggcg catcgatcct cgaacagaga aggtcctcga gcgtctcgag atgcctcccg
     2041 ggaccggcat ttcggggctc gagtccgatg gcggcgatct gttcttttgc ggagggggca
     2101 agagcggcaa ggtgaggacc gttcgacggc ccaagaaatc gggctaaatc acaggccaga
     2161 gccagaacgc cacgcccacc aatgcgatga atccgccact cagaacctcg ccatagcgct
     2221 ccagaggtcc aaggctgaca cgccgtaagc ccgcggtcga gtacacgcaa agcagaacgt
     2281 acgtggcgat ggttgtcgtg ccgaacacca gcgacatgag gatgatgagg ccgattccgt
     2341 ac
//
LOCUS       DASWZT010000066         4610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_19851, whole genome shotgun sequence.
ACCESSION   DASWZT010000066 DASWZT010000000
VERSION     DASWZT010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19851"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1461)
                     /locus_tag="VGE92_01760"
     CDS             complement(4..1461)
                     /locus_tag="VGE92_01760"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEY1312567.1"
                     /translation="MRPKGVLVNQLMSQVVEFAPNGVAVTTADGRIVLSNVELERMFG
                     YSRRQILELNIGRLIPERFRSSHAVLRDGHPSRFESRTVGAGRELFGLRANASEFPIE
                     IGVSVVQTAEGPLVVETIVDISVRKRLERMFQKVVEAAPCGMVMVDAQGRIMLVNTQS
                     EAMFGYQRAELIGNGLEMLLPERLRATHKDHRRTFAATPAIRQMGGLDADLTARRKDG
                     SEFPVEIGLNPVPGEDEGLVLATVTDMTQRKSIELKLRQANADLEEFTSAASHDLKSP
                     LRGISNLVEWIVEDLGPEAAPAVTRNLARVSDRIHRLERIIEDLLTYARAGVTAAAAV
                     PVDLHELIAGIVLILAPPSSFRIRVHVEAQPCVAAKAPLETVLRNLISNAVKHHDRAD
                     GELVIRIADEGRYCLFTIADDGPGIPQAAQEGAFRMLHTYSSNEREHSGIGLALCKRM
                     VAANGGRIQLESHDGVRGATFRVWWPRFQWVSHEQ"
     gene            1817..2887
                     /locus_tag="VGE92_01765"
     CDS             1817..2887
                     /locus_tag="VGE92_01765"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY1312568.1"
                     /translation="MQLEVNAVLTKQSAEDELEDSILAERLEERRRCRVLVVDDDELV
                     RARLSSVLQGAHYDVELASTGEEALRILARTDCHLLLTDWQMPDMDGLALCRQVRLAH
                     QDRYIYVLMLTVRNTKNDLLQGLASGADDYVVKGAPISEILARLEVGRRITHVDHSLR
                     AANQENKRLAFVDQLTQAYNLRYFMKYLPRELNRAQRYCRPLAVLSCDIDEFKRINDR
                     FGHQAGDEVLRRFVERCDASIRQGCDWIARVGGDEFIIVLPETTLQGAAHVDRKVRQQ
                     FAAEPLATSAGPIQISVSIGVTAVDAKHQEQSSARMHDLIQAADRRMYDDKRRKAGRL
                     PPAPGLGFDALQLPPGNKHGIN"
     gene            2874..3260
                     /locus_tag="VGE92_01770"
     CDS             2874..3260
                     /locus_tag="VGE92_01770"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY1312569.1"
                     /translation="MGSTRKCSAPQAHLAGPPDDFDDLRRAFEARMRSERIQFVALSA
                     ALASRGDDPEAIFGDLEFRAHKLRGAAQIFEAIEFAHAANALEQAAIAASRTHAGNAD
                     PAVWAALGDLVSLIGNLGITEIEIDV"
     gene            3460..>4610
                     /locus_tag="VGE92_01775"
     CDS             3460..>4610
                     /locus_tag="VGE92_01775"
                     /inference="COORDINATES: protein motif:HMM:NF017773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY1312570.1"
                     /translation="MLNAARRFILCAALYAGCAQATVFDLCVRGASLEETRTARLQFD
                     VQGADVSGFAPRLGAVLANRRFWDNSAEQVFGSVYDADLCGEEVAPLLQLKIEITPQQ
                     AKDLGAEAARGSQSGFLAAVDHAFGPAPAPQRPAFATRSFGSVPGKDANPDVGTPVVD
                     ANGKLRYHVVKIYYATNRNQTASPLPDEHYGRDRAPLSYGFVNVALPKAHEAGHLEAP
                     SIWRLEFSADPEKHVMLQSIKVMGEDAWRGEIAKRATALDNPGILVFIHGYNTSFPDA
                     ALRAGQLAYDLKFAGATVLFSWPSRAVTLEYTADEQEAEYSYTDMKTLLGSLSTIAPG
                     TPIYVIAHSMGNRVFTNGFKALMVEQPGKQSVFKQIVLAAPDIDADVFRR"
BASE COUNT          894 a         1457 c         1418 g          841 t
ORIGIN      
        1 gggttactgc tcatgactga cccactgaaa tctcggccac cacacgcgga aggtcgcgcc
       61 gcgcacaccg tcatgcgatt cgagctgtat ccggccgcca ttcgccgcca ccatgcgttt
      121 gcacaacgcg agtccgatgc cagaatgctc gcgttcattg gaggagtacg tgtgaagcat
      181 gcgaaatgcc ccctcctgag cggcctgcgg aattccgggt ccgtcgtccg cgatcgtgaa
      241 caagcaatat cggccctcat cggcgatgcg aatgacgagc tcaccgtcgg cgcggtcgtg
      301 gtgctttacc gcgttgctga tcagattgcg cagcaccgtt tcgaggggtg ccttggccgc
      361 aacgcagggt tgggcttcga catgcacgcg gatcctgaag cttgacgggg gagcgaggat
      421 cagaacgatt ccggcaatca attcatgcag gtcgacggga accgccgcgg cggcggtgac
      481 gcccgcgcgc gcgtaggtga gaagatcttc gatgatgcgt tccaagcggt ggatgcggtc
      541 gctgacgcgc gcgagattgc gggtgaccgc cggtgcggcc tcgggcccca agtcctcgac
      601 tatccactcg accagatttg atatgccgcg caacggcgat ttgagatcgt gcgatgccgc
      661 ggatgtgaac tcttccaagt ccgcattggc ctgccgcagc ttcagctcga tcgacttgcg
      721 ctgagtcatg tcggtcacgg tcgccaacac cagtccctcg tcctcgcccg gcaccggatt
      781 caggccgatc tccacgggga actccgagcc gtccttgcgc cgtgcggtca aatcggcgtc
      841 gaggccgccc atctgccgga tggccggcgt cgccgcaaac gtgcgccggt gatctttgtg
      901 cgtggcgcgc aggcgctccg gcagcagcat ctccaggcca ttgccgatca gttcggcgcg
      961 ctgataaccg aacatcgctt cagactgggt gttgaccaac atgatgcggc cctgtgcgtc
     1021 gaccatgacc atgccgcagg gcgcggcctc gacgactttt tggaacattc gttccaggcg
     1081 cttgcgcacc gaaatatcga cgatggtttc aaccaccaac ggtccttcgg cggtctgcac
     1141 cacgctcacg ccgatttcga tcggaaattc gctggcattg gcgcgcaagc cgaacaattc
     1201 gcgcccggcg cccacggtgc gcgattcgaa tcgactggga tggccgtcgc gcagcaccgc
     1261 gtgactgctg cgaaatcgtt caggaatgag ccgtccgatg ttcaactcga gtatttgccg
     1321 gcgtgagtag ccgaacatcc gttcgagctc gacattgctc aacacgattc gaccgtccgc
     1381 cgtcgtcacc gcaacgccgt tcggtgcgaa ctccaccact tgtgacatga gctggttgac
     1441 gagtactcct ttgggtcgca cgaatcgcat cgcggacgcg gcatctattc gccacccccg
     1501 gagatcccta tagcggtgcc gagtataagc ccgtagcggc gcagcggtgt cattacaaag
     1561 tcaagcgcgg cgccgcgcga tttcgagcgc gcgatgactt gcgggaatcc cttaaccgcg
     1621 agtcccgcac gcttgaatgt cgcggcggtc gacgatcaca gcgtcgtacg cgaatgatcg
     1681 aacgccagct cgacagtacg tgacgcactg cgcatttccg agcgaaatgc ggcaattgac
     1741 acaatcgccc tacagccact caagccgccg cggactataa atcgttgcat tgcctagcac
     1801 ttacccggag agtggcatgc agcttgaagt gaacgcggta ttgactaagc aaagcgcgga
     1861 ggatgagctg gaggattcca tcctggccga acgcttggaa gagcggcgtc ggtgccgcgt
     1921 gctcgtggtc gatgacgatg agttggtcag ggccaggctg tcatccgtgc tccaaggcgc
     1981 ccactacgat gtcgagttgg ccagtaccgg cgaggaagcg ctgcgcatat tggcacgcac
     2041 cgactgccat ctgctgctca ccgattggca aatgcccgac atggatggac tggcactgtg
     2101 ccgccaagtg cgcctcgccc accaggatcg ctatatctac gtactgatgc tcacggtccg
     2161 caacaccaag aacgacctcc tgcagggact tgcgtcagga gccgacgact atgtcgtcaa
     2221 gggcgctccc atcagtgaga ttctggcgcg cctcgaggtc ggccgccgca tcacgcacgt
     2281 cgatcactcc ctgcgtgcag ccaatcaaga gaacaagcgc ctggcgttcg tcgatcaact
     2341 gacccaagct tataatttgc ggtatttcat gaaatatttg ccgcgcgagc tcaatcgcgc
     2401 gcagcgctac tgccgcccgc tcgccgtgtt gagttgcgac atcgacgagt tcaagcggat
     2461 caatgaccgc ttcggtcacc aagcgggcga tgaggtgctg cgccgcttcg tcgagcgctg
     2521 cgatgcatcg attcgccaag gctgcgactg gattgcccgg gtgggcggcg acgaattcat
     2581 catcgtcctg cccgaaacca ccttgcaggg cgcagcgcac gtcgaccgaa aggtgcgcca
     2641 acaatttgcg gccgaaccgc tcgcgaccag cgccggcccg atccagatca gcgtgagcat
     2701 cggcgtaacc gcggtcgatg ccaaacatca agagcaaagt tccgcacgaa tgcacgatct
     2761 gattcaggca gccgaccggc gcatgtatga cgacaagcgt cgcaaggccg gtcgtttgcc
     2821 gccggcgccg ggcttagggt tcgatgcgct gcaattaccg ccgggaaaca agcatgggat
     2881 caactaggaa atgctcggcg ccgcaagcgc acctcgccgg gccgccggat gacttcgatg
     2941 atttgcgtcg cgccttcgag gcgcggatgc gaagcgagcg catacagttc gtcgccttga
     3001 gcgccgcgct cgcgagccgc ggcgatgatc ctgaagcgat attcggcgat ctggaatttc
     3061 gcgcgcacaa gctgcggggc gccgcgcaga tcttcgaagc catcgagttc gcccatgccg
     3121 ccaacgcctt ggaacaagca gcgatcgcgg cctcgaggac gcacgccggc aatgcggatc
     3181 ccgccgtatg ggccgcgctc ggcgatctgg tgagcctcat cggaaacttg ggcatcacgg
     3241 aaatagaaat cgacgtctga gacggccgcc cattcggccg cctgtaatcc ttaaaaaaat
     3301 gtaattcaaa tacaacggtt tacgtgccca atcagcggcc ggtgcgcatc cgcgctgcgg
     3361 tgcgcaagct tgcgacacga tatgctgtgc agacgtggcg gggagcagcg ctgcgggata
     3421 accttaacac cacgacaagc catgctcctg gagccgctta tgttgaatgc cgcccgtagg
     3481 ttcattctgt gcgctgcgct ctacgccggc tgcgcgcagg cgacggtgtt cgatctgtgc
     3541 gtgcgcggcg catcgctcga ggagacgcgc accgcccgcc tgcaattcga cgtccaaggt
     3601 gccgacgtct cgggcttcgc accgcgctta ggggcggtac tcgcgaatcg ccgcttctgg
     3661 gacaatagcg ccgagcaggt attcggctcc gtgtacgacg ccgacttgtg cggcgaagaa
     3721 gtggcgccgc tgctgcagtt gaagatcgag atcacccctc agcaggccaa ggatctcggt
     3781 gccgaagcgg ctcgcggttc acagagcggt tttctcgcag cggtcgatca tgcgttcgga
     3841 cccgcaccgg cgccgcagcg gcctgcgttt gcgacccgca gcttcggctc cgtccccggc
     3901 aaggacgcca accccgacgt cggcacgccg gtcgtcgacg cgaacggcaa gctccggtat
     3961 cacgtggtca agatctacta cgccaccaac cgcaatcaaa cggcctcgcc cttgcctgat
     4021 gagcattacg gccgcgaccg ggcgccgctg tcttacgggt tcgtgaatgt ggcgctgccc
     4081 aaggcgcacg aggccggaca tctcgaggcg ccgtcgatct ggcggctcga gttcagcgcg
     4141 gatccggaaa aacacgtcat gctgcagtcg atcaaggtga tgggggagga tgcgtggcgc
     4201 ggcgagatcg ccaagcgcgc caccgcgctc gacaatccgg gaatcctggt attcattcat
     4261 ggctacaaca ccagctttcc ggatgcggcg ctgcgcgccg gacagctcgc gtacgacctg
     4321 aaattcgccg gtgcgaccgt gctgttctcg tggccgtcgc gcgccgtcac gctcgaatac
     4381 acggccgatg agcaagaggc cgaatattcg tacacggaca tgaaaaccct gttgggctcg
     4441 ctctcgacca tcgcgcccgg cacaccgatc tacgtgatcg cgcacagcat ggggaatcgc
     4501 gtattcacca acggcttcaa ggcgctgatg gtcgagcaac cgggcaagca aagcgtcttc
     4561 aagcaaatag tgctggcggc gccggacatc gatgcggacg tgttccggcg
//
LOCUS       DASWZT010000067         2356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_87545, whole genome shotgun sequence.
ACCESSION   DASWZT010000067 DASWZT010000000
VERSION     DASWZT010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87545"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VGE92_01780"
     CDS             <1..334
                     /locus_tag="VGE92_01780"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEY1312571.1"
                     /translation="AGNLLASSAVPAAMIAAFEQARGHLGGRLLQSLRAGLDAGGEAG
                     PVHSAGLLIVREVSWPIVDLRVDWTEEDPVEALSAIWQRYAPQVDDYVRRAVDPVRAP
                     NFAVPGNP"
     gene            345..1853
                     /locus_tag="VGE92_01785"
     CDS             345..1853
                     /locus_tag="VGE92_01785"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY1312572.1"
                     /translation="MAATAVIRHPDLARLVQARDWRGALDRLDRALGVEPHDAHLHLT
                     RAHCLLALGRLRDAGAAAAQALRNAPHDPKMLDAIGSLLNRANDQRRALVAYDRAVAL
                     APDNPHFIFNRAAVQRFVGNLAAAEADYDRVIALDPRDHEAYKNRSDLRSQTANDNHV
                     AELEALAARGIPDWKGEVQIRYALAKECEDIGDYAQSFEHLQLGAGARRAHLRYDVAA
                     DVATVDWIIQAFPAVGAVGAVGAVGASRCAGASGEDPIFIVGLPRSGSTLVERILGSH
                     STLFAAGELTCFALSIVAAVHRLSGAAPLPRRDLIARSAQLDFAALGREYLERARHTM
                     PAGVRFTDKMPLNYLYCGLIVKALPNAKIVHVARRPLAACYAMYKTLFQDGYPFSYDL
                     TEIGRYYIGYRRLMDHWQAALPGFIHTVSYESLIADQAGEMRRLLDFCGLDWQDACAQ
                     FHLSAAPTTTASAAQVRRPLYDSAVAQWRHYTAELAPLRGQLQAAGIHTEDG"
     gene            1850..>2356
                     /locus_tag="VGE92_01790"
     CDS             1850..>2356
                     /locus_tag="VGE92_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEY1312573.1"
                     /translation="MREEISSIERHTIYPIPAAERHGRAGDLFTVWFGSNIMILTIAT
                     GALATTRFQLSLIAAVVAILIGNLVGGVFMALHAAQGPQLGVPQMVQSRGQFGSFGAI
                     FVVALVVIMYLGFVASNLVLGGQSLHSIAGGISDRTGIVLITILSLAAAIFGYDLIHA
                     YARWMTWVC"
BASE COUNT          385 a          738 c          786 g          447 t
ORIGIN      
        1 cgccggaaat ttgctggcga gcagcgcggt gccggcggcg atgatcgcgg cattcgagca
       61 ggcgaggggg catttgggcg ggcgtctgct gcagagcttg cgcgcgggac tcgatgcggg
      121 cggggaagcc gggccggttc attccgcggg ccttctgatc gtgcgcgagg tgagctggcc
      181 gatcgtcgat ttgcgcgtcg actggaccga agaggatccg gtggaggctc tcagcgcgat
      241 ctggcaacgc tacgcgccgc aagtcgatga ttacgtgcgc cgcgcggttg atccggtgcg
      301 agcgccgaat ttcgcagttc cgggcaatcc gtgagcgggc tccagtggcg gcaacggctg
      361 taattcggca tccggacctg gcgcgcttgg tgcaggcgcg cgattggcgc ggcgcgctgg
      421 atcggctcga tcgagcgctc ggcgttgaac cgcatgatgc gcacctgcat ttgacccgtg
      481 cgcactgcct gctggcactc ggtcgattgc gggatgccgg tgccgccgcg gcgcaggccc
      541 tgcgaaatgc cccgcacgac ccgaagatgc tcgatgcgat cggcagtctc ctcaatcggg
      601 ccaacgacca gagacgcgcg ctggtcgcgt acgatcgcgc cgtagcgctt gcgccggaca
      661 atccacactt cattttcaac cgagcggcgg tgcagcgatt cgttggcaac ctggccgcgg
      721 cggaggcgga ctacgaccgt gtcattgccc tcgacccgcg cgatcatgag gcatacaaga
      781 accgctccga cctgcgcagc caaacggcga acgataatca cgttgccgaa ctcgaggcgc
      841 tcgccgcgcg cggcataccc gactggaaag gcgaggtgca aattcgttat gcgctggcca
      901 aggagtgcga agatatcggc gactacgcgc agtcattcga acatctgcaa ttgggggccg
      961 gcgcacgccg cgcgcatctg cgctacgacg tcgccgccga tgtggcgacg gtcgattgga
     1021 tcatacaagc gtttccggcc gtcggcgctg tcggcgctgt cggcgcagtc ggcgcgagca
     1081 ggtgcgccgg cgcgtccggc gaggatccga ttttcatcgt cggtttgccg agaagcggta
     1141 gcaccctggt cgagcggata ctcggcagcc actcgacact cttcgcggcg ggggagctga
     1201 cttgttttgc gctgagcatc gtcgccgccg tgcacaggct gagcggcgct gcgccgctgc
     1261 cgcgccgcga ccttatcgcg cgatccgcgc agctcgactt tgcggccctc ggccgcgaat
     1321 accttgagcg ggcgagacac accatgccgg ccggcgttcg ctttacggac aagatgccgc
     1381 tgaattacct gtactgcggg ttgatcgtca aggccttgcc gaacgcgaaa atcgtgcacg
     1441 tggcgcgccg gccgctggcg gcatgctacg ccatgtacaa aaccctgttt caggatggct
     1501 acccgttttc ctacgatttg acggaaatcg gccgctacta catcggctat cggcgcttga
     1561 tggaccactg gcaggcggcg ttgcccgggt tcatccatac cgtgagttac gagtccctta
     1621 tcgccgatca agcgggggaa atgcgccggc tcctcgattt ctgcggtctt gattggcagg
     1681 acgcgtgcgc gcagtttcat ctcagtgcgg cgccgacgac cacagccagt gccgcgcagg
     1741 tgcggcggcc gctatacgat tccgccgtcg cccaatggcg gcactacacg gccgagctcg
     1801 cccccttgcg cggccagctg caggcggcag gcattcacac ggaagacgga tgagggaaga
     1861 gatctccagc atcgagcggc acacgattta tccgatcccg gcggccgagc ggcacggccg
     1921 cgccggcgat ctgttcacgg tgtggttcgg ctcgaacatc atgattctca ccatcgccac
     1981 cggcgccttg gcgaccaccc gttttcagct gtcgttgatc gcagcggtcg tggctattct
     2041 catcggaaat ctcgtgggcg gcgtgttcat ggcactgcac gccgcccagg gaccgcaatt
     2101 gggcgttccg cagatggtgc aaagccgcgg tcagttcggc tcgttcggcg ccatcttcgt
     2161 ggtcgcgctc gtcgtcatca tgtacctggg atttgtcgcc tccaatttgg tgctgggtgg
     2221 acaatccctg cacagcatcg cgggcggcat cagtgatcgg acgggaatcg tcctgattac
     2281 gattctgagc ctcgcggccg caatctttgg ctacgatttg attcatgcct acgcgcgctg
     2341 gatgacgtgg gtttgc
//
LOCUS       DASWZT010000068         4673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_53766, whole genome shotgun sequence.
ACCESSION   DASWZT010000068 DASWZT010000000
VERSION     DASWZT010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53766"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="VGE92_01795"
     CDS             <1..812
                     /locus_tag="VGE92_01795"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY1312574.1"
                     /translation="WAIDPEFLRADAVKIYADGVIEYPSQTAALLEPYLDAAGQQTHN
                     RGPSYFAQDNLNQIVTAADAAGLTVHIHAIGDRAIRSSLDAFAAARRRNGALDNRDQI
                     AHLELIDPADIPRFKELNVIANFQLLWAERDDYIDGSTIKYLGPQRSRYLYPARSVRD
                     AGAFIVGGSDWNVSSFNAFIAMEHAVTRSKGRGEPTLLPEQSLTLQDMLDAYTINAAA
                     ALRQERITGSLEPGKRGDFIVLDRDIFAIDAFDLHNTRVTLTYLDGREVYP"
     gene            980..1954
                     /locus_tag="VGE92_01800"
     CDS             980..1954
                     /locus_tag="VGE92_01800"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY1312575.1"
                     /translation="MAAPINTHATGDDLNLKDDLWAPLPETVRLGGHGPFVINLSASG
                     SPLAIPANPVQGCPQAHVYRVQRIEDGRARYRLRFGPFATEDEADAILKVVRDNYPSS
                     LTATADAEDLRVLAAIQSKTAAVVVPAPVAAAAPPAVATAPAVPTPAVAKPVMPKPAA
                     AKPKQTKAAPSGAKPIAAPPAGADTDAVQALKVLELEEDKDWRWYVIQLSVAEEPFDP
                     ATLPNLDIFRVYRLYCIAGIVDGRIRHALRVGFFAEQPAAVAVAGYLAAFYDKPVIKR
                     VSATERERFAEKSLEPRKDVGTTGKQAVIEITNERFVRAARSGQSAVK"
     gene            complement(1956..2972)
                     /locus_tag="VGE92_01805"
     CDS             complement(1956..2972)
                     /locus_tag="VGE92_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1615 domain-containing protein"
                     /protein_id="HEY1312576.1"
                     /translation="MTPEQSRNLIEHSLPAAAEDRPGWAADMYAAFTVLGLDTTRENV
                     CAVVAVIEQESGFRVDPVIAGLPTIAWREIDSRAERAGVPHLLVHAALQLTSPTGQTY
                     SDRIDHAKTEKDLSDIYEDFIGAVPLGRTLFAERNPIRTRGPMQVNIAFAEQYTAAKS
                     YPYPVKSSIADEVFTRRGSLYFGTAHLLAYPASYDSYLFRFADFNAGQYASRNAAFQS
                     ALSFAAGIPVAADGALLPHGKEKNPGGTELAARALGKRLDLSDGAIHDALEQGKAAQF
                     SQTALYQRVYALAERREGRALPRAAVPQIQLHGPKISRSLTTSWYAHRVEQRFRRCLT
                     SAAG"
     gene            complement(3074..3673)
                     /locus_tag="VGE92_01810"
     CDS             complement(3074..3673)
                     /locus_tag="VGE92_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312577.1"
                     /translation="MTCLRTLAAIVGCALFPALLFSPPSFADDASAHEPGVWQKHQYS
                     FAYMGFTSTYSCDGLADKLKLLLLAAGARRDVKSQAGACASGFGRPDKFARADLTFYT
                     LAPGSDGAAAAGDKPVDGTWRAVSFADRSPRDLHTGDCELVEQFRNSVLPMFTTRNID
                     NHTTCVPHQESGSNISLRFEAFAAPPAAAHAAKAAHGGT"
     gene            3940..>4673
                     /locus_tag="VGE92_01815"
     CDS             3940..>4673
                     /locus_tag="VGE92_01815"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312578.1"
                     /translation="MTLKFPSSVANLTGVAAEAAQMAVEAGLVYVSDAEPGIRRQRVG
                     KGFRYLTPENRRLAEAKELKRIASLAIPPAYRDVWITLQERGHLQATGRDARGRKQYR
                     YHPEWRQVRDSAKFDRMVAFGEALSKLRRKLKRDLGLPGLPREKVLAVVVSILDATRV
                     RIGNSEYARDNKSFGLTTLRNRHVSFIRDGRAILNFRGKGGVQHEVHIDDRRIAQIVR
                     RCQEIPGQHLFQYVSDDGQRCPIDSG"
BASE COUNT          892 a         1444 c         1496 g          841 t
ORIGIN      
        1 agtgggcgat cgatcccgaa ttcctgcgcg ccgatgccgt gaaaatctac gccgatggcg
       61 tcatcgagta tccgtcgcag accgccgcgt tgctcgagcc ctatctcgat gccgcggggc
      121 agcagacaca caatcggggt ccttcatact tcgcgcagga caatttgaat cagatcgtga
      181 ccgccgccga cgccgccggc ttgacggtgc acattcacgc catcggcgat cgcgcaattc
      241 gttcgtcatt ggatgcgttc gccgcggccc gacgccgcaa tggcgcgctc gacaaccgcg
      301 accagatcgc gcatttggaa ctgatcgatc ccgccgatat tccgcgcttc aaggaattga
      361 acgtgatcgc caatttccaa ctgttgtggg ccgaacgcga tgattacatc gacgggtcga
      421 caatcaaata cctggggccg cagcgctcac gctatctcta cccggcgcgc tcggtgcgcg
      481 atgccggcgc cttcatcgtc ggcggcagcg attggaacgt cagcagcttc aacgcgttta
      541 tcgcgatgga gcatgccgtc acccgcagca aggggcgcgg tgagcctacg ctgttgccgg
      601 agcagagcct cacgctgcag gatatgctcg atgcctacac gatcaatgcg gcggcggcgc
      661 tgcgccagga gcgcatcacc ggcagtctcg aacccggcaa gcgcggcgat ttcatcgtgc
      721 tcgatcgcga cattttcgcc atcgacgcgt tcgatctgca caatactcgt gtgacgctca
      781 cgtacctcga cggccgcgag gtgtacccat gagcctgtgg ttcgcttggc cggacataac
      841 gcgccggatt gttgcacttc acaacgatca ggcttgacat ataccgcgcg ccgtcgtcgt
      901 gcggagacgc cggtcacgga ttcatacgac gcgcttcgac actattcccc cgccctccag
      961 ccgttcgtgg aaattattca tggccgcgcc gatcaatacg catgcaaccg gggatgattt
     1021 gaatctcaag gacgacttgt gggcaccctt gcccgagacg gtgcgcctgg gtggccacgg
     1081 acccttcgtg atcaatttga gcgcgtcggg ctcgccactt gcgatcccgg cgaatccggt
     1141 tcaaggctgc cctcaagcgc atgtgtatcg cgttcaaagg atcgaggatg gacgcgcgcg
     1201 ttaccgactg cggttcggcc cgttcgctac ggaggacgaa gcggatgcca tcttgaaagt
     1261 agtgcgcgac aactacccaa gctccctcac ggcgactgcc gatgcggagg acttgcgtgt
     1321 gctcgccgca atccagtcga agacggccgc cgtcgttgtg ccagctccgg tcgctgctgc
     1381 cgctccgcca gccgttgcga cggccccggc cgtgcccacg ccggccgtgg cgaagcccgt
     1441 catgccgaaa ccggcggccg caaagccgaa gcagacaaag gcagcgccca gcggcgcgaa
     1501 gccgatcgcc gcgccgcctg ccggcgccga caccgatgcc gtgcaggcgc tgaaggtact
     1561 cgagctcgag gaggacaagg actggcgctg gtacgtgatc caattgtcgg tggccgagga
     1621 gccgttcgac ccggccacgc ttcccaattt ggacatcttc cgcgtttatc gcttgtattg
     1681 catcgccgga atcgtcgacg gacgcatacg gcacgcgctg cgcgtcggct tttttgcgga
     1741 acagccggct gctgtcgcgg tcgccgggta tttggcggcg ttttacgaca aaccggtcat
     1801 caagcgcgtc agcgcgacag aacgggagcg ctttgccgag aaatctctgg agccccgaaa
     1861 ggacgtgggc accaccggca aacaggcggt gatcgagatc accaacgaac gattcgtgcg
     1921 tgccgcccgc agcgggcagt ccgccgtcaa atgagttagc ccgccgcgga ggtgaggcag
     1981 cgacgaaagc gctgctcgac gcggtgcgcg taccagctgg tcgtcaaact gcgcgagatt
     2041 ttcggaccgt gtaattgtat ctgcggcacc gcggcgcgag gcaaggcgcg gccctccctg
     2101 cgctcggcga gcgcgtacac gcgctgataa agcgcagtct ggctgaactg cgctgcctta
     2161 ccctgctcga gggcgtcgtg aatggcaccg tccgaaagat cgagccgctt gccgagcgcg
     2221 cgcgcggcca attcggtgcc gcccggattt ttctctttgc catgcggcag aagcgcaccg
     2281 tccgccgcga ccgggatccc ggcggcgaag ctgagggcgc tttggaacgc ggcgttacgg
     2341 ctggcgtatt gacctgcgtt aaagtccgca aaccggaaaa gatacgaatc gtatgacgct
     2401 gggtaggcca gcagatgcgc ggtgccgaaa tacaggctgc cgcgccgagt gaatacttca
     2461 tccgcaatac tcgacttcac cggatagggg taggatttcg cagctgtgta ttgttcggca
     2521 aacgcgatat tgacttgcat gggtccgcgc gtgcggatcg gatttcgctc ggcgaacagc
     2581 gtgcggccca gcggcaccgc gccgatgaag tcttcataga tgtcgctgag atccttttca
     2641 gttttggcat ggtcgatgcg atcgctgtag gtctgccccg tgggcgaggt cagttgcaac
     2701 gccgcgtgca ccagcaggtg cggcacgccg gcgcgttcgg cgcggctgtc gatctcccgc
     2761 caggcgatcg tcggtaaccc ggcaatcacg gggtcaacgc gaaatcccga ttcctgctcg
     2821 atcacggcga ccacagcgca gacgttctcg cgagtggtgt cgaggccgag aacggtgaac
     2881 gcggcgtaca tgtcggcggc ccagcccggg cgatcttcag cggccgccgg cagcgaatgt
     2941 tcaatcaaat tgcgggattg ctccggggtc atacccgcgg ctgatggcgc cggcggtccg
     3001 ctggcgcaac ccgtcatcca ggacatcgcc gccaatatcg gcaggacgta gcggctccga
     3061 tgtgatgcgc cggctacgtt ccaccgtgcg cggctttcgc tgcatgcgca gcggcgggcg
     3121 gcgcggcaaa agcttcaaat cgcagactga tgttcgaacc ggactcctga tgcggaacac
     3181 aggtcgtatg gttgtcgatg ttgcgggtag tgaacatcgg cagcacggaa ttgcgaaact
     3241 gttcgacgag ctcacagtcg cccgtgtgca gatctcgcgg cgaacgatcg gcgaacgaca
     3301 ccgcccgcca ggtgccgtcg accggcttgt cgcccgctgc tgcagcgccg tcgctgcccg
     3361 gcgccagcgt atagaacgtg aggtcggccc gcgcgaattt gtccgggcgg ccgaacccgg
     3421 aggcgcaggc gccggcctgc gacttcacgt cgcgccgcgc acccgcagcg agcaacaaca
     3481 gcttgagctt atcggccagt ccgtcgcacg aataggttga ggtgaagccc atgtaggcga
     3541 aagagtactg atgtttctgc caaaccccgg gctcgtgggc cgaggcatcg tcggcgaagc
     3601 tggggggtga aaaaagcagg gcagggaaaa gcgcgcagcc gacaatggcc gccaaagtcc
     3661 gcaaacaagt catgcgagtc cctccgagtt gacgcggtat tgtatccgct aacataacaa
     3721 cgaacgaggt agtcgatagg ttgaccgcgc agaagtttga gagtgtccgt ccgactggcg
     3781 gacggaccaa cccgttgcgt ccgcagagct tgtgacgggc gtaggacttc cctgacagta
     3841 tgttctaact attgcgtcac agaattgcgt cacagaatta cgagctcggc gcgccagtcg
     3901 ctagcgcgcg gagtttcgaa tggctaacaa tccgacgcca tgactttgaa gtttccatct
     3961 tcggttgcaa atctcaccgg cgtcgccgcg gaagcggcgc agatggcggt cgaggccggg
     4021 ctcgtctacg tatcggacgc cgagccgggg attcgccgtc agcgcgtcgg caagggattt
     4081 cggtatctga caccggagaa ccgccgactc gccgaggcga aggaactgaa gcggattgcg
     4141 agtctcgcca ttccgccggc ctatcgcgac gtatggatca ccttgcagga gcgcggtcac
     4201 ctgcaggcga ccgggcgtga tgcgcgcggc cgtaagcaat accgctacca ccctgagtgg
     4261 cggcaggtgc gcgacagcgc aaagttcgac cgcatggtcg ctttcggcga ggcattgtcg
     4321 aaactgcggc gcaaactcaa gcgtgacctg gggttgcccg gactgccgcg cgagaaagta
     4381 ctggctgtgg tcgtcagcat tctggacgcg acgcgagtgc gcatcgggaa cagcgaatat
     4441 gcgcgcgaca acaagagctt cggcctcacc acgttgcgca accgtcatgt cagcttcatt
     4501 cgagatggtc gtgcgatctt gaatttccgc ggcaagggcg gggtgcagca cgaagtgcac
     4561 atcgacgaca gacgcattgc gcagatcgta cggcggtgcc aggagattcc gggtcagcac
     4621 ctgttccagt atgtgagcga cgacggccag cgttgtccga tcgactccgg tca
//
LOCUS       DASWZT010000069         1988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_34390, whole genome shotgun sequence.
ACCESSION   DASWZT010000069 DASWZT010000000
VERSION     DASWZT010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34390"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            675..1226
                     /locus_tag="VGE92_01820"
     CDS             675..1226
                     /locus_tag="VGE92_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312579.1"
                     /translation="MNDGASKATAPSITARPTPQEKRLFAAVAAQRQMSESTLALIAI
                     RALLDSQSPDLAPNPSNSGPALDRITIRLRPGDRLAIRHRAAERRMKDSAYIAALVRG
                     HVAVNPPLATGELAAFKAAVSVLAALGRILARAAREATQAGVLTRDLQEELRRSRALV
                     ADVERRMHEFAQAALVTWESRVD"
     gene            1219..>1988
                     /locus_tag="VGE92_01825"
     CDS             1219..>1988
                     /locus_tag="VGE92_01825"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEY1312580.1"
                     /translation="MTRRVVRLSSDEPPWFDVVSYGRAGPQMPHRFSPAQIGQISRTV
                     RRTPEVMVKVTGGGTRRGAVAAHFAYISRRGALEIETDEGERIGRPDEQKRLLDDWHL
                     ELTAGQYRRQVDGGPARRPTKLVHNIVLSMPAPTHPDIVLAAARKFARQKFAQHRYAM
                     VLHTDQKHPHVHLVVKAENELGRRLHIDKQLLRDWREYFARLMREQGIAANATPRAIR
                     GENKKKPRNEIFKAQRYGKSTALRDRVIGIAQELARSKT"
BASE COUNT          392 a          608 c          626 g          362 t
ORIGIN      
        1 gcagaaagca aatgaccgct ttccgatatt cattgcaatg tgtggtagta ccgcttttgg
       61 ccgggacctg cccgtggcac cccgacgcat cacgttgcac cctatgcggc tcacgacgga
      121 atcagattct cggcgacgga actagccagt attcgtccgc caattcatac gaatcatcgc
      181 cttattcgat tccaaccggt agctcatagt catgttagtg agttaaccgg agaactcgtc
      241 atggatcgtg cgttgtattc gatcgaagag acccgagcgc gtctcggtgg catttcgcgc
      301 aacagccttt atgccatgtt gagggctggc gaccttccga gcgtcgtcat cggttgccgc
      361 cgcttcatat cggcggaggc gattgctgcg ctcatcaccg attccacgac atcgatcagc
      421 ccatctcagg acgcggtacg gtcacggcgg cctctggtta atgtaggcca ggggcttcgt
      481 tcgaccgctc gacccagcac tcatcctcgg aagtccgacg ccgagtaagt attcggaccg
      541 ggcgcaatga gttcgtggat gcaatcggga ctgcgctgtc gtcgcttaca tcgaggagcg
      601 ttgtcgggtc ggtattggag tgagggcagg atagcgaagt gtccgcgatt tgcagcaaca
      661 cagattggcg cgcagtgaac gacggtgcct cgaaggcgac tgcgccgtcg atcacggcgc
      721 gtccgacgcc gcaggagaag cgcctgttcg ctgcggttgc tgcccagcga caaatgtcgg
      781 aatcgacgct ggcactgatc gccattcgtg cattgctcga ttcccaatcg cccgacttgg
      841 cacccaaccc atcgaacagc ggtcccgccc tggaccggat cacgatccgt ctgcggccgg
      901 gagaccggct cgccatccgc caccgagcgg ctgagcgccg catgaaggac tcagcctaca
      961 tcgccgccct ggtccggggt catgtggccg tcaacccgcc gctggcaacc ggcgagcttg
     1021 cggcgttcaa ggcggccgtg tcggtcttgg ctgccttagg cagaatcttg gcgcgggcgg
     1081 ctcgggaagc cacgcaggcg ggcgtcctga cacgagatct ccaggaagaa ctccgccgtt
     1141 ctcgggctct cgtagcggac gtagagcggc gtatgcatga attcgcccaa gcggcgctgg
     1201 tcacctggga gagccgcgtt gactagaaga gttgttcgcc tctcgtcgga tgaacctcct
     1261 tggttcgacg tggtgagtta cggtcgcgcg ggcccgcaaa tgccccatcg attcagcccg
     1321 gcgcagattg gccaaatctc gcgcacggtc aggcgcacgc cggaggtcat ggtgaaagtg
     1381 actggcggag ggacgaggag gggtgcggtt gcggcccact tcgcgtacat cagccgccgc
     1441 ggagccctgg agatcgagac cgacgagggg gagcgcatcg gacgcccgga tgagcaaaag
     1501 cggctgctcg atgactggca cttggaactc accgccggcc aataccggcg gcaggtggac
     1561 ggcggtcccg cgcgacgacc gacgaaactc gtgcacaaca tcgtgctgtc tatgccggcg
     1621 ccgacgcacc cagacattgt gttggccgcc gcgcggaaat tcgcccgcca gaaatttgcg
     1681 caacatcgct acgccatggt tttgcacacc gaccagaagc acccgcacgt gcatctggtc
     1741 gtgaaggccg agaacgagct tggccggcgg ctgcacatcg acaagcagct cctgcgagat
     1801 tggcgcgagt acttcgcccg attgatgcgc gaacagggta ttgccgccaa cgccacgccg
     1861 cgggccattc gaggcgagaa caagaaaaaa ccccgaaacg agattttcaa agcgcagcga
     1921 tacgggaagt ccactgcgct gcgcgatcgg gtgatcggga tcgcgcaaga gcttgcacgt
     1981 tccaagac
//
LOCUS       DASWZT010000070        12169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_63452, whole genome shotgun sequence.
ACCESSION   DASWZT010000070 DASWZT010000000
VERSION     DASWZT010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12169
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63452"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VGE92_01830"
     CDS             <1..382
                     /locus_tag="VGE92_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312581.1"
                     /translation="LPWQAVMAASIAVIAVALSLLAAERWVQFRGRAAPADYYTVTSP
                     APHASGEVIRAVFSPAITLVELQAILDEAQLRIVAGPSEAGVYSLAPNSRRPVGSALA
                     LLRAHPQVRFAERTQSNVAPGDAP"
     gene            379..1680
                     /locus_tag="VGE92_01835"
     CDS             379..1680
                     /locus_tag="VGE92_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY1312582.1"
                     /translation="MRPCAALLLVWVVSACSSTPVRIDARALSDQVERSPEQYIIAAI
                     DNDPGPFVARAGSTPRGYDGIAAYGPSHSARQMMLAVETDYGLREVSAWPIEPLHMHC
                     AVLEVPKGADRASLLATLAHDQRIKLAQPLQSFATRTAAYNDPFVELQRGFQQMDVAG
                     AHPWSRGDGVKVAIIDTGADTKHPDLLGNIEAAANFVDGDNLKFQRDRHGTEMAGVIA
                     AVANNREGIVGIAPRARLLLFKACWQIELDADAARCNSFTLARALAAALDAHAQIVNL
                     SLSGPADPLLSELIREGLRRGVLFVGAAGEFTHQPGVIEVASAGGAELGSALYAPGSE
                     ILTLLPGGHYDFASGDSIATAQVSGVLALLLARNSALSAAAAYRLLHDTSSPNGSPNG
                     ATALIDACAAVVSLIGQGSCSQGAAKVQKVAGEGGQAAALR"
     gene            complement(1691..2638)
                     /locus_tag="VGE92_01840"
     CDS             complement(1691..2638)
                     /locus_tag="VGE92_01840"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY1312583.1"
                     /translation="MSKTPVPVAQALARAPSQRLLGIAYMLAAVFVFAIMDALLKRLS
                     GHYGPLQVASLRCISSWLFLLPPIIGRGLWATLRPRQLSLHLLRAVLGIGMLGSFVFA
                     VHRLSLAQTYSLFLAAPLLMTALSVPILGETVTARRWLAIAAGMGGVLVILQPWGNRG
                     SSSFSLIAAAAAALATLCYSLSALTVRTLGRDNSSLSMVFWYLLMVAIGCGVLALGDW
                     RPILASDWSWLVAVGLTGALGQLWLTNAFSRAPPSVVAPFEYMALIWAFGIDWIFWSA
                     APSLSLVAGGCVVIAGGIVVLVDEHRLSRLALNPASPPP"
     gene            complement(2678..3442)
                     /locus_tag="VGE92_01845"
     CDS             complement(2678..3442)
                     /locus_tag="VGE92_01845"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY1312584.1"
                     /translation="MLKALLWDVDGTLAETERNGHLQAFNLAFEALHLPWRWSPEYYG
                     ELLAVAGGRERLLHDMQSQGRGPSGLEDRHALAQQVHVLKNRLYASIVAAGELPLREG
                     VRELLQDCAQAGLRVGIVTTTSRSNVAALLNAHLGRAWEERFATVVSADEAALKKPDP
                     QAYLLALQELKLRPHEALAMEDAPAGLAAAQAAGIPVIVTRSYYFPTTATHGALAVGP
                     SLGRPAGWNPAAESRAARIDLAQITRWFEHGKTLPR"
     gene            complement(3459..4496)
                     /gene="fba"
                     /locus_tag="VGE92_01850"
     CDS             complement(3459..4496)
                     /gene="fba"
                     /locus_tag="VGE92_01850"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116726.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEY1312585.1"
                     /translation="MAFISLRQLLDHAAEHSYGVPAFNVNNLEQIQAIMEAAEESQSP
                     VILQASAGARKYAGEPYLRHMVLAAIESHPAIPVVLHQDHGASPAVCTQSIRSGFTSV
                     MMDGSLQEDAKTPASYDYNVDVTRRVCDMAHTIGVSVEGELGCLGSLESGQAGEEDGV
                     GAQGTLTHDMMLTDPQQAQDFVTRTGVDALAIAIGTSHGAYKFTRQPTGDILAIDRIA
                     QIHAAVPNTHLVMHGSSSVPQEWLAIIREHGGDIKETYGVPVEEIQRGIRHGVRKVNI
                     DTDIRLAMTGAMRQAFARDPGEFDPRKALIAAKKAARLLCKARFEAFGCAGQAQRIQP
                     LALEMMARRYH"
     gene            complement(4512..5387)
                     /locus_tag="VGE92_01855"
     CDS             complement(4512..5387)
                     /locus_tag="VGE92_01855"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEY1312586.1"
                     /translation="MSIKHPIIAVTGSSGAGTTTVMKSFANIFRREQIKAQVIEGDSF
                     HRFNRVEMRERVRQADSGAGPAISHFGPEANLLDDLESNFAEYAETGGGKVRRYIHDA
                     SEAKELGGDPGTFTEWQPMAQHSDLLFYEGLHGGYKEGKTDVARHVDLLVGVVPIVNL
                     EWIQKLHRDQHVRGYSQDAVVDTILRRMPDYVNYICPQFSRTHVNFQRVPTVDTSNPF
                     VAKDVPSSDESLVVIRFANPKGIDFPYLISVLHNSWMSRPNNIVVPGGQMGLAMQFIF
                     TPMILRLMDLKRRAQ"
     gene            complement(5400..6479)
                     /locus_tag="VGE92_01860"
     CDS             complement(5400..6479)
                     /locus_tag="VGE92_01860"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF006779.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEY1312587.1"
                     /translation="MHLGRTTLSKFLIQQLSGIEGANDLGALLVDVAAAVKAISAMTA
                     KGALGGYLGELGSKNVQGEVQHKLDVLANDVMLRSCEWGGLVAGMASEELDDPYPVPS
                     EYPRGRYLLIFDPLDGSSNTDVNVSVGTIFSVLRHDRPTPPTTSDYLQPGRQQVAAGY
                     AIYGPATMLVVTVGKGTHGFTLDREIGNFILTHPNLQIPKDTSEFAINTSNARFWEPP
                     VHRYVTECQAGKTGDRARDFNMRWIASMVAEVHRILMRGGVFMYPRDTKDPGKPGRLR
                     LLYEANPITFLVEQAGGRATTGRERLLDVMPESLHQRVPLILGSQHEVERIERYHAEH
                     ASGSDRPYSSPLFNERSMFRSEVRF"
     gene            complement(6482..7456)
                     /gene="cbbX"
                     /locus_tag="VGE92_01865"
     CDS             complement(6482..7456)
                     /gene="cbbX"
                     /locus_tag="VGE92_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbX protein"
                     /protein_id="HEY1312588.1"
                     /translation="MAAPLYQIPGQAPPPGAAAPSAATAEAVPSPSADNCAARTVADV
                     LAQSQADTVIDELERDLVGLAPVKTRIRDIAALLVIDKLRMNLGLQAQAPSLHMSFTG
                     NPGTGKTTVALRMAQILHRLGYVRKGHLVAVTRDDLVGQYIGHTAPKTREVLKRALGG
                     VLFIDEAYYLYRPENERDYGQEAIEILLQVMENQRDDLVVILAGYKDRMDAFFRSNPG
                     MSSRIAHHLDFPDYPAEDLLRIAGKMLQQQNYHFGPGARAAFEEYLTRRIVQPHFANA
                     RSVRNALDRARLRQASRLFADKHKEFTPEDLSTIAEPDIRASRVFKGD"
     gene            complement(7463..7909)
                     /locus_tag="VGE92_01870"
     CDS             complement(7463..7909)
                     /locus_tag="VGE92_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose bisphosphate carboxylase small subunit"
                     /protein_id="HEY1312589.1"
                     /translation="MMTNPVGRITQGQFSYLPDLTDEEISMQIQYGLNKGYAWSVEYT
                     DDPHPRNTYWEMFGLPMFDLKDAAGVLMELQHCRKTFPQHYIRLMAFDSTRGIETIVM
                     SFIVNRPDKEPGFGLVRQEAPGRTIRYTVHSYATDRPETERGTGGA"
     gene            complement(7927..9405)
                     /locus_tag="VGE92_01875"
     CDS             complement(7927..9405)
                     /locus_tag="VGE92_01875"
                     /inference="COORDINATES: protein motif:HMM:NF003252.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="form I ribulose bisphosphate carboxylase large
                     subunit"
                     /protein_id="HEY1312590.1"
                     /translation="MLKSIEKPAEVAIKDAKKRYTAGVLKYRQMGYWDADYAPKDTDT
                     LCLFRITPQEGVDPIEAAAAVAGESSTATWTVVWTDRLTACDSYRAKAYKVEPVPNNP
                     GQYFAWVAYDIILFEEGSIANMTASLIGNVFSFKPLKAARLEDIRVPVAYVKTFKGPP
                     TGLIVERERLDKFGRPLLGATTKPKLGLSGRNYGRVVYEGLKGGLDFMKDDENINSQP
                     FMHWRDRFLNVMDAVNKASAATGEVKGSYLNVTGATVEDMYERAEFAKELGSVIIMID
                     LVVGWSTIQSMSNWCRKNDMILHLHRAGHSTYTRQKSHGVSFRVIAKWLRLAGVDHMH
                     TGTAVGKLEGDPMTVQGYYNVCRDSVTKQDLPRGLYFDQDWADLRKVMPVASGGIHAG
                     QMHQLLDLFGDDVVLQFGGGTIGHPGGIQAGAVANRVALEVMVKARNEGRDIKNEGPQ
                     ILRRAAKTCSPLQQALDTWGEISFNYASTDTSDYSATPAASN"
     gene            9505..10455
                     /locus_tag="VGE92_01880"
     CDS             9505..10455
                     /locus_tag="VGE92_01880"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY1312591.1"
                     /translation="MKNATLRQLRVFASVARHLSFIRAAEELNLSAPAVSMQIKELET
                     EVGLPLFDRTSRQVSLTMVGEYMLAHTRRVLSAMRDAEDLVARFRGLQTGPLDVGMVS
                     TAKYFLPRLLAQFRQEHPGIEIRLQVANNREQIVHLMQEGDIELAIMGRPPEGYVTRA
                     EPFAMHPHVLVTSIEHRFTRLEVVPARALADEGFIVREPRSGTRAAFDEYMRTHQVVP
                     HVVMQMSSNEAIKQAVMAGMGISLLSLHTLGLELNHHLIAAPDTEGLPVMRRWHVVNN
                     QAKTLSPAAEAFRYFILERGEAFLAQQFPSSALLHSNANK"
     gene            10469..11674
                     /gene="pcaF"
                     /locus_tag="VGE92_01885"
     CDS             10469..11674
                     /gene="pcaF"
                     /locus_tag="VGE92_01885"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008173805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEY1312592.1"
                     /translation="MMRHAYICDALRTPFGRYGGVLSGIRTDDLAALPLRALLARHPG
                     VDWEAVSDVILGCANQAGEDNRNVARMAALLAGLPQSVGGSTVNRLCGSGLDAVGTAA
                     RAIRSGESNLAIAGGVESMSRAPFVVPKAQIAFSRSSEIYDTTIGWRFINPLMKAQFG
                     VDSMPETAENVAREFGIERAAQDRMALASQQKAAAAQRAGFFDAEITPVTIQRSKTEP
                     LLVTQDEPPRETSLQALARLKGVVHPEGTVTAGNASPINDGACALLLADESGAKKHGL
                     EPRARVLGMASAGVAPRLMGMGPAPATRRVLALAGLTLDQMDVIELNEAFAAQGLAVL
                     RDLGLKDDDARVNPNGGAIALGHPLGASGARLVTTAVSQLQRTRGRYALCSMCVGVGQ
                     GVAMIIERC"
     gene            complement(11689..>12169)
                     /locus_tag="VGE92_01890"
     CDS             complement(11689..>12169)
                     /locus_tag="VGE92_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312593.1"
                     /translation="VRVWHPGQRWIWQAGGLGVFDPGINALSILTRIIPGAWLLKSAE
                     LAVPSNCETPSAAHLELRGPAQTSVDLDFLQTGTQTWDIDVHTDAGHLRLSLGASIMA
                     IDDQPVDLPQTAEYPNLYAHFAALVKQGSSEVDVSPLQLVADAFLSGRRVAAAPFIE"
BASE COUNT         2174 a         3807 c         3860 g         2328 t
ORIGIN      
        1 cctgccatgg caggctgtga tggcggcttc gattgcggtg atcgccgtgg cgctgtcgct
       61 gttggcggcc gagcgctggg tgcagttccg cgggcgcgcg gcgccggccg actattacac
      121 ggtgacctca cccgcgccgc atgcctcggg cgaggtgatt cgagcggtgt tctccccggc
      181 cattaccctg gttgaattgc aggcaattct ggacgaggca caactgcgaa tcgttgccgg
      241 accgagcgag gcgggagtgt attcgttggc gccgaactcg cggcgcccgg tgggcagcgc
      301 gctcgccttg ctgcgcgcac acccgcaggt tcgtttcgcc gagcgtacgc aatccaatgt
      361 ggcgccgggc gatgcgccat gaggccgtgc gcggcgttgc tcttggtatg ggtcgtttcc
      421 gcctgcagca gcacgccggt gcgcatcgat gcgcgtgcgc tatccgatca ggttgagcgt
      481 tcacccgagc agtacatcat cgcggcgatc gacaacgacc caggcccttt tgtggcgcgc
      541 gccggcagca cgccgcgcgg ctacgacggc atcgccgcct atggaccgag tcacagtgcg
      601 cgccagatga tgcttgccgt cgaaacggac tacggcttgc gtgaagtgag cgcgtggccc
      661 atcgagccgc tgcacatgca ttgcgcggtg ctcgaggttc ccaagggcgc ggatcgcgcg
      721 agcctgttag cgaccctggc gcacgatcag cgcatcaagc tggcgcagcc gctgcagagt
      781 tttgcaacac gcaccgccgc ctataatgat ccctttgtgg agttgcagcg cggcttccag
      841 caaatggatg tggccggcgc gcacccttgg tcgcgaggcg acggcgtcaa ggtcgcgatc
      901 atcgatacgg gcgccgatac caagcacccg gacttgctcg gcaacatcga ggctgccgcc
      961 aatttcgtgg atggcgacaa tctcaagttt cagcgcgatc ggcacggcac cgagatggcg
     1021 ggtgtcatcg cagcggtcgc caacaatcgc gaaggcatcg tcggaattgc cccgcgcgcg
     1081 cgcctgttgt tgttcaaggc gtgctggcag atcgaactcg acgccgatgc cgcgcgctgt
     1141 aattccttta ccttggcccg ggcgctggcg gcggcactcg atgcgcatgc gcagatcgta
     1201 aacttgagcc tttccggccc ggcggatccg ctgctcagcg aactgattcg cgaaggtttg
     1261 cgccgcggcg tgttgttcgt cggcgccgcc ggcgaattca cgcatcaacc gggggtcatc
     1321 gaagtcgcga gcgccggagg tgcggaactc ggttccgcgc tgtacgcccc gggcagcgaa
     1381 attctcactc tcttgcccgg cggccactat gatttcgcat ccggagattc gattgcaacc
     1441 gcccaggtga gcggggtgtt ggcgttgttg ctcgcaagga attccgcact ctcggccgcc
     1501 gccgcttacc gtctgttgca cgataccagc tcgccgaacg gctcgccgaa cggcgcgacg
     1561 gcgctgatcg acgcctgcgc ggcggtcgta tcgctgattg gccagggatc ctgcagccaa
     1621 ggcgccgcga aggtgcagaa agtcgcgggc gagggcggtc aagcggcggc gctgcgctag
     1681 cggccgtcga tcacggcggt ggactcgcgg ggttgagggc cagccggctc aagcggtgtt
     1741 cgtccacgag caccacgatg ccgcccgcga tgacgacaca gcctccggca accaagctca
     1801 gcgacggcgc agccgaccaa aatatccagt cgatcccgaa cgcccagatc aaggccatgt
     1861 actcgaaggg tgcgacaacc gagggcggcg cgcggctaaa ggcattcgtc agccatagct
     1921 ggccgagagc accggtgagg ccgaccgcga cgagccagct ccaatcggac gcgagaatcg
     1981 gccgccagtc gccgagcgcg agcacaccgc acccgatcgc gaccatcagc aggtaccaaa
     2041 acaccatcga caggctcgaa ttgtctcgtc ccagggtgcg tacggtgagg gcgctcagcg
     2101 aatagcacag cgtcgcgagc gccgcggctg cggcggcgat cagcgaaaag ctgctgctgc
     2161 cgcggtttcc ccacggctgc agtatcacca gcacgccgcc cataccggcg gcgatcgcta
     2221 gccagcgacg cgccgtcacc gtctcgccca gaatcggaac cgagagcgcg gtcatcaaca
     2281 acggcgccgc caagaacagc gaatacgttt gcgccaggct caagcggtgc accgcgaata
     2341 cgaagctgcc cagcatcccg atcccgagca cggcccgcaa caaatgcagc gacagttgcc
     2401 ttggccgcaa cgttgcccac aaacctcggc cgatgatcgg cggcagcaga aacaaccagg
     2461 atgaaatgca gcgaagagac gcgacctgca gcggcccata atgcccggac aagcgcttca
     2521 gcagcgcatc catgatcgcg aacacgaata ccgcggccag catgtaggca atgccgagca
     2581 gccgttgcga gggcgcgcgt gcgagggctt gggcgacggg gacgggcgtc ttcgacattc
     2641 gaattccgcc atagtaaacc agacggcgcc gccggcgtta tcggggcagc gttttgccgt
     2701 gctcgaacca acgagtaatt tgcgcaagat cgatgcgcgc cgcgcgtgac tctgccgcgg
     2761 gattccagcc ggccggccgg ccgagcgatg ggccgaccgc caaggcgcca tgcgtcgcgg
     2821 tcgtgggaaa gtaatagctg cgggtcacga tcaccggtat cccggcggct tgcgccgcgg
     2881 ccaggcccgc cggtgcgtcc tccattgcga gcgcctcgtg cgggcgcagt ttaagctcct
     2941 gaagcgcgag caagtaagcc tgcggatcgg gctttttcag cgctgcttcg tcggcggata
     3001 cgaccgtggc gaagcgctct tcccatgccc ggccgaggtg cgcattgagc agcgccgcga
     3061 cgttcgatcg gctggtggtg gtcacgatac cgacgcgcag cccggcctgc gcgcagtcct
     3121 gcaacagttc gcgcacaccc tcgcgcaacg gcagctcgcc cgcggcgacg atgctcgcgt
     3181 agagtctatt cttcaggacg tgaacttgct gcgcgagagc gtggcgatcc tcgagccccg
     3241 aggggccgcg accttgagac tgcatgtcgt gcagtaagcg ctcgcggccg ccggctacgg
     3301 cgagcagctc gccgtagtat tcggggctcc agcgccacgg caggtgcagc gcctcaaagg
     3361 ccaaattgaa cgcctgcaga tgtccgttcc gctctgtttc cgccagggtg ccatccacgt
     3421 cccagagcag cgccttgagc atcgagccgg acgccgtctc agtgataacg cctggccatc
     3481 atctcgagcg ccagcggctg gatgcgctgc gcctgacccg cgcagccaaa ggcctcgaag
     3541 cgcgccttgc acagcaggcg tgccgctttc ttcgccgcga tcagtgcctt gcgcggatcg
     3601 aattcgccgg gatctcttgc aaaagcctga cgcatcgctc ctgtcatggc gagacgaatg
     3661 tccgtgtcga tattcacttt gcgcacgccg tggcggatgc cgcgctgaat ttcctccacg
     3721 ggaacgccgt aggtctcctt gatgtcaccg ccgtgctcgc gaatgatcgc gagccattcc
     3781 tgcggcacgc tcgaggagcc gtgcatgacc aagtgagtat tggggactgc cgcatggatt
     3841 tgcgcgatcc ggtcgatggc cagaatgtcg cccgtgggtt gacgggtgaa tttgtaggcc
     3901 ccgtggctgg tgccgatggc gatggcaagc gcatcgacgc cggtgcgcgt cacgaagtcc
     3961 tgcgcctgtt gcgggtccgt cagcatcatg tcatgggtca gagtcccttg cgcgccgacc
     4021 ccgtcctcct cgccggcttg accgctttcc aaagaaccga ggcagccgag ctcaccctcg
     4081 accgacactc cgatggtatg cgccatgtcg cacacacggc gcgtcacgtc gacgttgtag
     4141 tcatagctcg ccggcgtctt tgcatcctcc tgcaatgaac cgtccatcat gacgctggta
     4201 aatccagagc gtatcgactg cgtacacacc gccgggctcg cgccgtgatc ttgatgcagc
     4261 accacgggaa ttgccggatg cgattcgatc gcggccagta ccatgtggcg caggtacggc
     4321 tctccggcgt atttgcgggc gcccgccgag gcttgcaaga tcaccggact ttggctctct
     4381 tcggcagctt ccatgatcgc ttgaatctgc tcgagattgt tgacgttgaa tgccggcacg
     4441 ccgtagctat gttccgcggc gtgatcgagc agttgacgaa gggaaatgaa tgccatggat
     4501 actcctaacg gttattgcgc gcgccttttc aggtccatca agcgcaggat catgggggta
     4561 aagatgaatt gcatcgccag acccatctgc ccgcccggta ccacgatatt attcggccgg
     4621 ctcatccagg agttgtgcag caccgaaatg aggtacggga agtcgatccc tttgggattg
     4681 gcaaatcgga tcaccaccaa cgactcatcc gagctcggga cgtccttggc aacaaaagga
     4741 ttgctggtat cgacggtcgg cactcgctgg aaattcacgt gagtgcggct gaattgcggg
     4801 catatgtaat tcacatagtc cggcatgcgc cgtaaaatgg tatcgaccac cgcgtcctgg
     4861 ctgtaaccgc gcacatgctg atcgcgatgc agcttctgga tccattccag gttcacgatc
     4921 ggtaccacgc cgaccagcag atcgacgtgg cgcgcaacat ccgtcttgcc ctccttgtag
     4981 ccgccgtgca agccttcgta gaacaacaag tcgctgtgtt gcgccatcgg ctgccactcg
     5041 gtgaaggtgc cgggatcgcc gccgagttcc ttggcttcgc ttgcatcatg aatgtagcgc
     5101 cgcaccttgc cgccgccggt ctccgcgtat tcggcgaaat tcgactcgag gtcatccaac
     5161 agattcgcct cgggtccaaa atggctgatc gccgggccgg caccgctatc ggcctgacgc
     5221 actcgctcgc gcatctcgac gcggttgaag cggtgaaatg aatcgccttc gatgacctgt
     5281 gccttgattt gttcgcgccg gaatatgttc gcgaagcttt tcatcacggt ggtggttccc
     5341 gcgccggacg atccggtcac cgcaatgatg ggatgcttga tggacatggg ttgctttcct
     5401 taaaaccgca cttcggagcg aaacatcgag cgttcgttga acagcggcga gctgtacggg
     5461 cggtccgagc cgctggcgtg ttccgcgtga tagcgctcga tacgttcgac ttcatgttgc
     5521 gagcccagaa tcaacggtac tcgctgatgc aacgattcgg gcatgacgtc gagcaagcgc
     5581 tcgcgtccgg tggtggcgcg cccgccggcc tgctcgacca ggaaagtgat cggattggcc
     5641 tcgtatagca gccgcaaacg gcccggcttg cccgggtcct tggtatcccg gggatacatg
     5701 aagacaccgc cgcgcatcag aatgcgatgt acttcggcga ccatcgaggc aatccatcgc
     5761 atgttgaagt cccgggcccg atcgccggtc ttgccggcct ggcattcggt cacgtaccgg
     5821 tgcaccggcg gctcccagaa tcgggcattg ctggtgttga tggcgaattc gctggtgtct
     5881 ttcggaatct gcaggttcgg atgcgtcagg atgaagttcc caatctcgcg atccagcgta
     5941 aagccgtgcg tacctttgcc gaccgtgacc accagcatgg tcgccgggcc ataaatcgcg
     6001 tagcccgcgg ccacctgttg gcgccccggc tgcagatagt cgctcgtggt cggcggcgtc
     6061 ggccggtcgt gacgcaacac cgagaagatg gtccccaccg agacattgac gtcggtgttg
     6121 gatgagccat cgagcggatc gaagatgagc aaataccggc cgcgcggata ctcgctcggc
     6181 accggatacg gatcgtccaa ctcttccgag gccatgccgg cgaccaatcc gccccattcg
     6241 cagctgcgca gcatcacatc gttcgcgagc acgtcgagct tgtgctggac ctcgccttgc
     6301 acattcttgc tgccgagctc gcccaagtag ccgccgagcg ctcccttggc ggtcatcgcc
     6361 gagatcgcct tgacggcggc cgcgacatcg accagcagcg cgcccaggtc gttggcaccc
     6421 tcgatgccgc tcagctgctg aatgagaaac ttcgaaagcg tggtgcggcc aagatgcatg
     6481 gtcagtctcc tttgaagaca cggctggcgc gaatatcggg ttcagcgatg gtcgagagat
     6541 cttcgggcgt gaattcctta tgcttgtccg caaacaggcg gctcgcctgc cgcagccgcg
     6601 cgcgatccaa ggcattgcgc acgctgcgcg cgttcgcaaa atgcggctgc acaatgcggc
     6661 gcgtgaggta ttcctcgaat gccgcgcggg cgcccggtcc gaagtgataa ttctgctgct
     6721 gcagcatttt gccggcgatg cgcagcaggt cttcggccgg atagtcgggg aagtccaagt
     6781 ggtgcgcgat gcgcgagctc atgcctggat tggaacggaa aaacgcgtcc attcgatcct
     6841 tgtatcccgc caagatcacc accaggtcat cgcgctggtt ctccatcact tgcagcagga
     6901 tctcgatggc ttcctgccca taatcgcgct cgttctcggg gcgatacagg tagtaggcct
     6961 cgtcgatgaa cagcacgccg ccgagcgccc gcttcagcac ttccctggtt ttcggcgccg
     7021 tgtgcccgat gtattgaccg accaaatcat cgcgtgtaac cgccaccaaa tggcccttac
     7081 gcacgtagcc caagcggtgc aggatctgcg ccatgcgcag cgccaccgtg gttttgccgg
     7141 tgcccggatt gccggtaaat gacatgtgta ggctgggggc ctgggcttgc agcccgagat
     7201 tcatgcgcaa cttgtcgatc accagcaacg cggcaatgtc gcgaatgcgc gttttcaccg
     7261 gcgcgagccc gacgagatcg cgttccagtt catcgatcac ggtgtcggcc tggctttgcg
     7321 ccagtacatc cgcgaccgtg cgcgcagcgc aattgtcggc ggatggacta ggcaccgcct
     7381 cggcggtggc ggcgctcggc gccgccgcgc cgggcggggg cgcctgtcca ggtatctggt
     7441 acaacggtgc ggccatgatg tcctaggcgc cgccggttcc gcgttccgtc tccggacggt
     7501 ccgtcgcgta gctgtggacg gtgtagcgaa tcgtgcgtcc tggcgcttcc tgtcgcacca
     7561 atccgaagcc gggctccttg tcgggacgat tgacgataaa actcatcacg atggtctcga
     7621 tgccgcgcgt cgaatcgaac gccatgagcc gaatgtagtg ctgcggaaat gtcttgcggc
     7681 agtgctgcag ttccatcagg acgccggcgg cgtctttcag atcgaacatc ggcaggccga
     7741 acatctccca gtaggtgttg cgcggatgcg ggtcatccgt gtattcgacg ctccacgcgt
     7801 agcctttgtt gagtccgtat tgaatttgca tggagatctc ctcatccgtg aggtcgggca
     7861 ggtaactgaa ttgtccctgc gtgatgcggc cgactgggtt ggtcatcatg gtgcgatcct
     7921 cgagagttag ttcgatgcgg caggggtggc cgagtaatcc gacgtgtcgg tcgatgcata
     7981 gttgaagctg atttcgcccc aggtatcgag tgcctgttgc agcgggctgc aggtttttgc
     8041 agcgcgccgg agaatctgcg gcccttcgtt cttgatgtcg cggccctcgt tgcgggcttt
     8101 gaccatgact tcgagcgcca cccggttggc gacggcccct gcttggattc cgcccggatg
     8161 gccgatcgtc ccgccgccga attgcagcac gacgtcgtcg ccgaacaaat cgagcagctg
     8221 atgcatctga ccggcgtgga tcccgcctga agcgaccggc atgactttgc gcaagtccgc
     8281 ccagtcttga tcgaagtaca ggccgcgcgg caggtcttgt ttcgttacac tgtcgcggca
     8341 aacgttgtag taaccctgca ccgtcatcgg atcgccttcg agcttgccca ccgcggtgcc
     8401 cgtgtgcatg tggtcgacac cggccaagcg cagccatttc gcgatcacgc gaaagctgac
     8461 gccgtggctc ttctgccgcg tgtaggtgct gtggccggcg cggtgcagat gcaggatcat
     8521 gtcgttcttg cggcaccagt tcgacatgct ctgaatggtc gaccagccga ccaccaagtc
     8581 gatcatgatg atgacggaac ccaattcctt ggcgaattcc gcgcgctcgt acatgtcttc
     8641 gacggtggcg ccggtgacat tcagatacga gcccttcacc tcgccggtgg ctgcgcttgc
     8701 cttgttcacg gcgtccatga cgttgaggaa gcggtcgcgc caatgcatga agggctgcga
     8761 gttgatgttc tcgtcgtcct tcatgaaatc gagaccgccc ttgagcccct cgtaaacgac
     8821 gcggccgtag ttgcgcccgg acaggccgag cttcggtttg gtcgtggcac cgagcagcgg
     8881 acggccgaat ttatcgaggc gctcgcgctc gacgatgagt ccggtcggcg gccccttgaa
     8941 ggtcttgacg tacgcgacgg gaacccggat gtcttcgagc cgcgcggcct tgagcggctt
     9001 gaagctgaat acgttgccga tgaggcttgc cgtcatattg gcgatcgagc cttcttcgaa
     9061 caggatgatg tcgtaggcga cccaggcaaa gtactgaccc gggttattcg gcaccggctc
     9121 gactttgtag gccttggcgc ggtagctgtc gcaggcagtc aaccgatcgg tccataccac
     9181 cgtccaggtc gccgtgctcg actcccccgc gaccgcggca gcggcctcga tgggatcgac
     9241 accctcttga ggagtgatcc tgaacaggca cagggtgtcg gtgtccttag gagcgtagtc
     9301 ggcgtcccag tagcccatct ggcggtactt gaggaccccg gccgtatagc gcttcttggc
     9361 gtctttgatg gcaacttctg cgggtttttc gatcgatttc agcatttgag agcctcgcct
     9421 tgactgttgg ggcaagggac tgtatgcggg gttgatcgtt tcagtacagt cagttattgt
     9481 tatcccaatg ttaagtgttg cttaatgaag aacgccacgc tacgacagtt gcgggtgttc
     9541 gcgtcggtgg cccggcatct gagcttcatt cgcgccgcag aagagctcaa tctttcggcg
     9601 cccgccgtct ccatgcaaat caaagaactc gagaccgagg tgggattgcc gctgttcgat
     9661 cgcaccagcc ggcaggtctc gctcaccatg gtgggcgagt acatgctggc gcatacccga
     9721 cgagtgctgt cggccatgcg cgatgccgaa gatctggtgg cgcgatttcg cggcttacaa
     9781 accgggccgc tcgatgtcgg catggtcagt accgccaaat actttttgcc gcgattgctc
     9841 gcgcaattcc gccaagagca tccgggcatc gaaatccgct tgcaagttgc caataaccgc
     9901 gagcagatcg tgcacctgat gcaagagggc gatattgaac ttgccatcat gggcagaccg
     9961 cccgagggat acgtgacccg cgccgaaccc ttcgccatgc atccgcacgt actcgtgact
    10021 tcgatagaac atcgcttcac gcggctcgag gtggtgccgg cgcgcgcgct ggcggacgaa
    10081 ggcttcatcg tgcgcgaacc gcggtccggc acgcgcgccg cattcgacga gtacatgcgt
    10141 acgcaccagg tggtgccgca cgtggtcatg caaatgtcca gcaacgaagc gatcaagcag
    10201 gccgtcatgg ccggcatggg catctcgctg ctgtcgctgc atactctagg acttgaactc
    10261 aatcaccatt tgatcgcagc gccggacacg gaaggattgc ccgtcatgcg ccgttggcac
    10321 gtggtgaaca accaggcgaa gacgctgtcg cccgccgcgg aggcatttcg ctatttcatt
    10381 ctcgagcgcg gcgaggcgtt tcttgcgcaa cagttcccga gctccgcgct attgcactca
    10441 aacgccaaca agtagacaag gaatctccat gatgcgccac gcctatatct gcgatgcctt
    10501 gcgaacgccc ttcggtcgct acggcggtgt cttgtcgggc attagaacgg atgatttggc
    10561 cgcgctgccg ctgcgcgcgc tgttggcgcg ccaccccggc gtggattggg aagcggtttc
    10621 cgacgtcatc ctcggatgcg cgaatcaagc gggcgaagac aaccgcaatg tggcccgcat
    10681 ggcggccctg ttggccggac tgccgcagag tgtcggcgga tccaccgtca accgtttgtg
    10741 cggctcgggt ctcgatgccg tcggcacggc tgcgcgcgct atccgcagcg gcgaatccaa
    10801 tctggcgatc gccggcggag tcgagagcat gagccgggcg ccatttgtgg ttccgaaggc
    10861 gcagatcgcg ttttcccgca gcagcgagat ctacgacacg accatcggtt ggcgcttcat
    10921 caacccgctg atgaaagcgc agttcggcgt cgattcgatg cccgagaccg ccgaaaacgt
    10981 cgcgcgcgag ttcggcatcg agcgcgccgc gcaggaccgg atggcgctcg cgtctcagca
    11041 gaaggcagcc gccgctcagc gagccggatt cttcgacgcc gaaatcacgc cggtcacgat
    11101 ccagcggagc aagacggagc cgttgctcgt cacccaggat gagccgccgc gcgaaacatc
    11161 gctgcaagcg ctcgctcgac tcaagggagt ggtgcacccc gagggcaccg tcacagccgg
    11221 caacgcttcg ccgatcaacg acggcgcgtg cgctttgctg ctcgccgatg agagcggcgc
    11281 gaaaaaacat ggcttggaac cgcgcgcgcg cgtgctcggc atggcgagcg ccggggtcgc
    11341 gccccgtttg atgggaatgg ggccggcgcc ggccacccgc cgggtgttgg cgctggccgg
    11401 gctgacactc gatcagatgg acgtcatcga attgaacgag gctttcgcgg cgcagggtct
    11461 ggccgtattg cgagacttgg gactcaagga tgacgacgcg cgcgtcaatc cgaatggcgg
    11521 cgcgattgca ctcgggcatc cgctcggcgc cagtggggcg cgtctcgtga ccacggcggt
    11581 atcgcagttg cagcgtactc gaggacgcta tgctttgtgc tcgatgtgcg tcggcgtcgg
    11641 ccagggtgtc gccatgatca tcgaacgctg ctgattgcag cgcgccggct actcgatgaa
    11701 cggtgcggcc gcaacgcgcc gtccgctcaa gaacgcgtcg gccaccagtt gcagtggcga
    11761 aacatccacc tcgctcgagc cttgcttcac gagcgcggca aaatgtgcgt acaaattcgg
    11821 gtattccgcg gtttgcggca ggtccaccgg ctggtcgtcg atcgccatga tgcttgcgcc
    11881 cagtgacaac ctcagatgac cggcatcggt atgcacatcg atgtcccagg tttgagttcc
    11941 ggtctgcaga aagtccagat cgacggaggt ctgcgccggt ccgcgcagtt cgagatgcgc
    12001 ggcgctcgga gtttcgcaat tgctcgggac ggctagctcc gcgcttttca acagccaagc
    12061 gccgggaatg attcgggtga gaatcgacag cgcgttgatc ccggggtcga acacgccgag
    12121 cccgccggct tgccagatcc accgctggcc ggggtgccac acgcgcacg
//
LOCUS       DASWZT010000071         6616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_24733, whole genome shotgun sequence.
ACCESSION   DASWZT010000071 DASWZT010000000
VERSION     DASWZT010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6616
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24733"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VGE92_01895"
     CDS             <1..191
                     /locus_tag="VGE92_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312594.1"
                     /translation="ATPAGPFAQVQTEFQGSQAQALAALIAAGSSPAAAEQSLVQAYH
                     TSVAPFCMVAARGFFSEL"
     gene            203..910
                     /locus_tag="VGE92_01900"
     CDS             203..910
                     /locus_tag="VGE92_01900"
                     /inference="COORDINATES: protein motif:HMM:NF015857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HEY1312595.1"
                     /translation="MTTFSMRTLPAALAATVLGLYFATPAYCDDYPANSLRFGLYSVF
                     YHVSAEDLQGPYVPAGANIDAKNVETLYLAYVRSLTPNLDFELTAGYPPLQKTVGKGP
                     ATLGSVPYNGQVIATARWIAPTALLEYVFFDPSARLRPYIGAGVNYSTFYDRDSTAAG
                     DAASGGPTKLSLSRSIGPAGTVGLAWNLGSNWHMYGSYSISRVNSDLTADTAGVIRTT
                     QIKFGPQALVVALGYSF"
     gene            928..1857
                     /locus_tag="VGE92_01905"
     CDS             928..1857
                     /locus_tag="VGE92_01905"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEY1312596.1"
                     /translation="MSEPGAPPPTYRSGTQSVAADEAAATPQERAAALAFLQALAAEV
                     SAGSIDLPCFPDVVVRISMALADPNTSADHVVTVVGAEPRLAARILQTANSAAFNTSG
                     KPLTELRSAITRLGHQMVHGTAMAYAVHQMKHESALKSIVKPMTDLWNKSIAVASISR
                     IVARGTKLTPDEAFLTGLLHGIGTLYIIARATTEGTPLGGEQSLADLLAGWHASIGKA
                     VLESWGFAEELCEAVGEQEDDDRRWRHEATLSDVLIASLLLAEFLDTPEPRAVVTEGK
                     SVFLTVGVRAEDCPAILAEAEQQIRLVHAALAS"
     gene            complement(1863..2756)
                     /locus_tag="VGE92_01910"
     CDS             complement(1863..2756)
                     /locus_tag="VGE92_01910"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY1312597.1"
                     /translation="MSTCSPPQESIEQRIRARGAVLDMPFTQAIYQPLLQQQRRDGVE
                     VERDLAYGEDARQKVDVYRPQAHAVQHRSAMIFMPGGGFIRGDKSERENVGQYFARHG
                     IVVAVANYRLAPAHTWPAGAEDVIAVFEWLQKNAARYGVDRTRIFLAGESAGAAHVAA
                     AMLIRRFHPPDGLSAAGGILISGVYNVRLEQLARAQFGIATPDPRNEAYFGSDFTRYP
                     AMSTIDLIDAAAFPLLITFAEIDLLQMQIQAGELFARLVTNHGFAPQLGVVRGHNHLT
                     QVYAVNTGDESLSAPMLEFVS"
     gene            complement(2763..3998)
                     /locus_tag="VGE92_01915"
     CDS             complement(2763..3998)
                     /locus_tag="VGE92_01915"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY1312598.1"
                     /translation="MILFVNSRIFDGSGAPPYAGEVLVDGNRIAAVTRAGEAPVSRDQ
                     ARVVDGAGATLMPGMTEAHAHLSWPSSVERFVPGMSLPPEDLLLNTARNARILLDHGF
                     TSAYSAGALGKTLEASLKAQIDSGGMPGPRLVPSSIEREPANDTAFLESGKVQEHGTG
                     PDAIRAFIKECAAVGAKVVKFLLSGESALKPGASLQILYTEDEIRAAGEQARESNVWL
                     SGHAHAAQAIKMGVRNGFRVLYHCTYADAEAIDMLAERKEDIFVGPTLGIVQATLDAK
                     PPPHFDMSHMKADAAVVLEHQKRLAPQLRKRGVRLLPGGDYGFPFNPNGRNARDLELF
                     VQHLGYTPAEALVAATQLGGEIMGMGAELGRIKAGYLADLLLVRGDPTQNVAILQDKE
                     NLLAIVKDGKFHKTPAAPV"
     gene            complement(4001..>6616)
                     /locus_tag="VGE92_01920"
     CDS             complement(4001..>6616)
                     /locus_tag="VGE92_01920"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEY1312599.1"
                     /translation="IYGVTLGLQHIGLPRFLGGSLTSAELQASDDGIHFRKLADIPTD
                     RGGLAQDPGQTTLALAPTTARFFRVLLSTPSGGAGTPGIDLSALGLAPVPPPSEYRVS
                     RFMLHTCAVVNHFEDKAAFAATPNLYAAPTPPAPADVVIAPGDVLDLTDHMSADGRLD
                     WSPPPGRWVVLRMGYSLTGAKNSPASPEATGLEVDKLDRNAVRTYLDQYLAQYQSASG
                     GLLGARGVGFVVTDSWEAGAQNWTPALFDEFKRRRGYDLHAWLPVLTGQVVQSAAASE
                     RFLWDFRETLGELLAQNHYGQLADSLRERGMGLYSESHENGRAFIGDGMQVKRHATVP
                     MGAMWTQKPGLDAELFGYNADVRESASVAHLYGQNLVAAESFTAGSGAWAWSPETLKP
                     TADKEMAMGVNRFVIHTSVHQPLIGKPPGLALGPFGQWFTRNETWAEQAAPWIRYLAR
                     SSYLLQQGRFVADVAYFYGEDSNVTALFAGKAPPVPAGYNFDFINADTVLTALRVDHG
                     ALTAPSGMQYRVLALDANAQHMSLPVLKRLAEFVADGAIIIGARPADTPSLADDAREF
                     AELAAQVWGEGSGRHSFGKGETLSGETLDVALAELGIAPDIEYRKRDSDSALLFVHRA
                     LADGDLYYIDSRNSRAQRVEVSFRVSGKAPELWHADSGEREAVSYRIVDGRTLVPLDL
                     GPYETVFVVFRRAGSAAGLSLPLRAESALANVTGPWQLRFQEGRGAPTGAIPISTLVS
                     WSDSADPGIKYFSGTATYTTTFKADAGWFARGASLWLDLGDIKNLADVTLNGHSLGIL
                     WKTPFRVDITDALKRGNNLLQIKVTNLWVNRLIGDQQPNAAQKITFTTQVFYKADAPL
                     LSSGMLGPVRIVQRR"
BASE COUNT         1191 a         2165 c         2034 g         1226 t
ORIGIN      
        1 acgctacgcc cgcggggccg ttcgcgcagg tgcaaaccga atttcaaggg agccaggcgc
       61 aagcgttggc ggcattgatt gccgccggct cgtcgccggc ggcagccgag cagtccctcg
      121 tgcaggctta ccacacctcc gtggcaccct tctgcatggt tgccgcccgc ggcttcttca
      181 gcgaactcta aggggaccgg tcatgacgac tttctcgatg cgcacgctgc cggccgccct
      241 cgccgcaacc gttctcggtc tgtacttcgc gacgccggcc tactgcgacg attaccccgc
      301 caatagcctg cgcttcggcc tgtactccgt gttctatcac gtgagcgcgg aggatctgca
      361 gggcccgtac gttccggcgg gcgccaacat cgatgccaag aacgtcgaaa cactgtacct
      421 cgcctatgtc cgaagcttga cgccgaacct cgatttcgaa ctgaccgcgg gttatccgcc
      481 gctgcagaaa accgtgggca agggcccggc gaccttgggc tcggtacctt ataacgggca
      541 agtgattgcg acggcccgct ggattgcgcc gactgcgctg ctcgaatacg tgttcttcga
      601 cccgagcgcg cggttgcgtc cgtacattgg tgccggcgtc aattactcga ctttttatga
      661 tcgggattcg acggcggcgg gcgatgccgc cagcggcgga ccgaccaagc tgtcgctcag
      721 ccgctcgatc ggaccggccg gcacggtcgg cctcgcgtgg aatctgggga gcaattggca
      781 catgtatggc tcgtacagca tcagccgggt gaattcggat ctcaccgcgg ataccgccgg
      841 cgtcattcgc acgacgcaga tcaaattcgg accgcaggca ctggtcgtgg cgctgggtta
      901 ttccttctag gcctcgcgtg cattcccatg tctgaacctg gggcaccgcc gccgacgtat
      961 cgatccggca cgcaatccgt ggccgccgac gaggctgcag cgacgccgca ggagcgggcc
     1021 gcggcgcttg cttttctgca agccctggcc gcggaggtct ccgcgggctc gatcgatttg
     1081 ccgtgctttc ccgatgtggt cgtgcgcatc agcatggcgt tggccgatcc gaacacctcc
     1141 gccgatcacg tggtcaccgt ggtgggtgcc gagccgcggt tggcggcgcg cattctgcaa
     1201 accgctaatt ccgcggcctt caatacatcc ggcaaaccgc tgacggagct gcgctctgcc
     1261 atcacccgct tgggacatca aatggtgcac gggactgcga tggcctacgc cgtgcatcag
     1321 atgaagcacg agagcgcgct caaatcgatc gtcaagccga tgaccgatct atggaataag
     1381 agcatcgcgg tcgcctcgat cagccgtatc gtcgcgagag gtaccaaact tacgccggac
     1441 gaggccttct tgaccggtct gttgcacgga atcggcactc tctacatcat tgctcgcgcc
     1501 acgaccgagg ggacgccact cggcggcgag caatcgctgg cggatcttct ggcgggctgg
     1561 catgcatcga tcggcaaggc ggtgctcgag agttggggct ttgccgagga actctgcgaa
     1621 gctgtcggcg aacaggagga tgacgatcgc cgctggcggc acgaggcgac gctgtccgat
     1681 gtgctgatcg caagtctgct gctcgcggaa ttcttggaca ctcccgagcc acgcgcggtg
     1741 gtgaccgagg gcaagagcgt atttctcacg gtgggcgtgc gagccgaaga ctgtcccgcc
     1801 attctggctg aggcggagca gcaaatccgc ctggtgcacg cggcgttggc aagctagcag
     1861 cctcagctga caaactccag catcggcgca ctcaatgatt catccccggt gttcaccgcg
     1921 tagacctgcg tcagatgatt atggccgcgg accacgccca attggggtgc gaagccatgg
     1981 ttcgtcacca gtcgggcaaa cagctccccc gcttggattt gcatctgcaa cagatcgatc
     2041 tcggcaaagg tgatcagcaa cggaaatgcc gcggcgtcga tcagatcgat ggtcgacatg
     2101 gcgggatagc gcgtgaagtc ggagccgaaa taagcctcgt ttctcgggtc cggagtggca
     2161 atgccgaatt gcgcccgcgc caactgctcg agtcgtacat tgtagacgcc tgagatgagt
     2221 atgcccccgg cagcggacag gccgtcgggc gggtgaaatc gtctgatcag catggccgct
     2281 gccacgtgcg cagcgcccgc cgattctccc gccagaaaga tccgtgtgcg atcgaccccg
     2341 tagcgcgccg cattcttctg cagccattcg aacaccgcga tcacgtcctc ggcgcccgcc
     2401 ggccatgtgt gcgccggtgc cagcctatag ttggcgaccg ccacgacgat gccgtgccgg
     2461 gcgaaatact gaccgacgtt ctcgcgctcg ctcttgtcgc cgcgaatgaa tcccccgccc
     2521 ggcatgaaga tcatcgcgct gcggtgttgc acggcgtgcg cctgcggccg atacacatcg
     2581 actttctgcc gcgcgtcctc gccataggca agatcacgtt ccacttcgac gccatcgcgc
     2641 cgctgctgct gcagcaacgg ctggtagatc gcctgcgtga agggcatatc gagcacggcg
     2701 ccgcgcgcgc ggatacgctg ctcgatcgac tcctgcggcg gggagcacgt gctcatattc
     2761 aattagaccg gcgcggccgg tgttttgtgg aattttccat ccttgacgat ggccagcaaa
     2821 ttttctttgt cctgcaggat cgcgacattc tgggtgggat cgccgcgaac caacagcaag
     2881 tccgcgagat accccgcttt gatccgaccc aactcggcgc ccattcccat gatttcaccg
     2941 cccaattgcg tggccgccac cagcgcctcg gcgggcgtgt agcccaagtg ctgcacgaac
     3001 agctccaggt cgcgggcatt gcgtccattc ggattgaatg gaaacccata atccccgccg
     3061 ggcaaaagac gcaccccgcg cttgcgaagc tgcggagcca gtcttttttg atgttccagg
     3121 acgaccgcgg catcggcctt catgtggctc atgtcgaaat gcggcggcgg ttttgcgtcc
     3181 aaagtcgctt gaactatgcc gagggtcggg ccgacgaaga tgtcctcctt gcgctccgcc
     3241 agcatgtcga tcgcctccgc gtctgcatag gtgcaatggt agagaacgcg aaagccgttg
     3301 cgcacgccca tttttatcgc ctgcgcggca tgtgcatgcc cgctgagcca cacattggac
     3361 tcgcgtgcct gctcgccggc ggcgcggatc tcatcctcgg tgtagagaat ttgcaatgat
     3421 gccccgggct tgagcgcgct ctcgcccgac agcagaaact tgaccacttt tgcgccaacc
     3481 gcggcgcact ccttgatgaa tgcgcggatt gcatcggggc ccgttccatg ctcttgaacc
     3541 ttgcccgact cgaggaatgc agtgtcgttc gccggctcac gctcgatcga ggatggcacg
     3601 agcctgggtc ccggcatgcc tcccgaatcg atctgcgctt tgagcgatgc ctcgagcgtc
     3661 ttacccaagg caccggcgga ataggcgctg gtgaatccat gatccaacag gatgcgggca
     3721 ttgcgcgcgg tattgagcaa cagatcttcg ggcggcaggc tcatgccagg cacgaatcgc
     3781 tccaccgaac tcggccagct caagtgcgcg tgtgcctccg tcattcccgg catgagcgtt
     3841 gcgcccgcgc cgtcgaccac cctcgcttgg tcgcgcgaca cgggcgcctc gccggcgcgc
     3901 gtcacggcgg cgatgcggtt gccgtcgacc aatacttcgc cggcataggg cggcgcgccg
     3961 ctgccgtcga aaatacgtga attgacaaac aggatcatgc tcatctcctc tgcacgatac
     4021 gcaccggtcc caacatcccg gaggacaaga gcggcgcatc ggccttgtag aatacctggg
     4081 tcgtgaatgt aatcttctgg gccgcgttcg gctgctgatc gccgatcaag cgattcaccc
     4141 agagatttgt caccttgatt tgcagcagat tattaccgcg cttcagggca tcggtgatat
     4201 cgacgcgaaa cggcgtcttc caaagaattc ccaagctgtg gccgttcaag gtcacatcgg
     4261 cgagattctt gatgtcgcct aaatcgagcc acagagaggc gccgcgagca aaccagccgg
     4321 cgtcggcttt gaaagtggtt gtgtacgtgg cggtgcccga aaaatacttg atgccaggat
     4381 cggctgaatc gctccacgat accagggtgc tgatcggaat tgcgccagtc ggcgcaccgc
     4441 ggccttcctg aaaccgcagt tgccacggac ccgttacatt cgcgagcgcg gactcggctc
     4501 gcagcggcaa gctgagaccc gccgccgagc ccgcgcggcg gaacaccacg aataccgttt
     4561 cataagggcc cagatccaac ggcaccagcg tgcggccatc gacgatgcga tacgacaccg
     4621 cttcgcgttc gccgctgtcc gcatgccaca gctccggcgc tttgccgctg acgcgaaagc
     4681 tcacctcgac tcgttgcgca cggctattgc ggctgtcgat gtagtacaaa tcgccatcgg
     4741 caagcgcccg gtggacgaac aacaaggcgc tatcgctgtc tcgcttgcgg tactcgatgt
     4801 ccggcgcgat tccgagctct gccaacgcga cgtccaaggt ctccccggag agggtttcgc
     4861 ccttgccgaa actatgccgt ccgctgccct cgccccacac ctgggccgcg agctcggcaa
     4921 actcgcgcgc gtcatcggcg aggctcggcg tatcggccgg ccgggcgccg atgatgatcg
     4981 caccgtcggc gacgaactcg gccaggcgct tgagcaccgg caatgacatg tgctgcgcat
     5041 ttgcgtcgag cgccagcact cggtattgca tgccgctcgg tgcggtgagc gcgccgtggt
     5101 cgacgcgcaa tgccgtgagc accgtgtcgg cattgatgaa gtcaaaattg tatccggccg
     5161 gcacgggcgg cgccttgccg gcaaacagcg ccgtgacgtt cgaatcctcg ccataaaaat
     5221 acgccacgtc cgccacgaag cgaccctgct gcaacaggta gctgctgcgc gcgagatagc
     5281 ggatccacgg cgccgcctgc tcggcccatg tttcgttgcg cgtgaaccat tgaccgaacg
     5341 gccccaatgc gagccccggg ggtttgccga tcagcggttg atgcaccgag gtgtgaatca
     5401 caaagcgatt cacgcccatg gccatttcct tgtcggccgt cggctttaag gtctccggcg
     5461 accaggccca ggcgccgctg cccgccgtga aggactccgc cgccacgaga ttctgaccat
     5521 agagatgggc caccgaggct gactcgcgca cgtcggcgtt gtagccgaac agctcggcgt
     5581 ccaagcccgg cttctgagtc cacatcgccc ccatcggcac cgtggcgtgg cgcttgactt
     5641 gcatgccgtc gccgatgaac gcgcggccgt tctcgtgcga ttcgctatac aggcccatgc
     5701 cgcgctcgcg cagcgaatcg gccagctggc cgtagtggtt ctgcgccaaa agctcgccca
     5761 aggtctcgcg aaaatcccac aaaaagcgct cgctggccgc ggcgctctgc acgacctgcc
     5821 cggtgagcac cggcaaccac gcatgcaaat catacccgcg gcggcgtttg aattcgtcga
     5881 acaaggcggg agtccagttt tgcgcgcccg cctcccagct atcggttacg acgaagccga
     5941 cgccacgcgc gcccagtaag ccgccgctcg cgctctggta ctgcgccaag tactgatcga
     6001 ggtaggtgcg caccgcgttg cgatcgagtt tgtccacctc gagtccggtc gcttcgggcg
     6061 acgccggcga attcttggcg ccggtcagcg agtatcccat ccgcagaacc acccatcgcc
     6121 ccggcggcgg cgaccaatcc aagcgcccgt cggcactcat gtggtcggtt aggtcgagca
     6181 catcgcctgg cgcgatcacg acgtccgcgg gtgctggcgg cgtaggcgcc gcgtatagat
     6241 tgggcgtcgc cgcaaacgcc gctttgtctt cgaagtgatt gaccaccgcg cacgtgtgca
     6301 gcatgaaccg cgacacccga tattcgctgg gcggcggcac cggcgccaaa ccgagggcgc
     6361 tcaagtcgat tcccggggtg cctgcaccgc cggatggcgt gctcaagagc acgcgaaaga
     6421 agcgcgccgt cgtcggcgcg agcgcgagcg tggtctggcc cgggtcctgc gccaacccgc
     6481 cgcggtccgt tggaatatcg gcgagcttgc gaaagtgaat tccgtcatcg cttgcctgaa
     6541 gttcggcact cgtcaacgat cctccgagga atcgcggcag gccgatgtgc tgcaggccga
     6601 gcgtcacccc atagat
//
LOCUS       DASWZT010000072         9006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_10274, whole genome shotgun sequence.
ACCESSION   DASWZT010000072 DASWZT010000000
VERSION     DASWZT010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9006
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10274"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..2724)
                     /locus_tag="VGE92_01925"
     CDS             complement(103..2724)
                     /locus_tag="VGE92_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1312600.1"
                     /translation="MQSFAHHATNSTTVRRDHLFFTSKGWTLMNPNQAKLRQSIQMIL
                     AATSMAVVSAPAFSQAGAAATATTAAATSDSAAADASDTLETIVITASAGDKSKLNTS
                     TSISSINQEQIQNFDATSTAELYRMIPGIQVSGTQGDGGNSNIGVRGLRTPTGGSPFV
                     QIQEDGLPVVLFGDIQFGNNDYWTHPMPTDERVEAIRGGTAATLASQAIGAVLNHISF
                     TGRNDGGFVELEKGANYDFTKVNFRLGSSINDTTYYNIGGYYDVGRGIEHAAYNVSDS
                     YAIKGNITKELADDKGYVRLLFKASDTQEPSYNGCISSATLTGHTVSNLGPSPYCDAR
                     NQAPGYSALNSSALFSNVSGDLARQPLNGILTDEKWLQVQTHYNYGGGLVIDDNARVA
                     RMSGAFDVQFYNATPTASALGAGQSLIYANGPNAGQAFTGAYVNNNAQVHTNMSHMDH
                     IANDLSASYSGDLSPAHFDVKVGYLYYSQRIAEDWHSNSNLQEASGVDPAELDLVSGL
                     NGTGNLLAAGGTSGFNQGWNQQFDLTFTDNAPYADLNVDVGNLNLDGSVREEFFQGSG
                     WAQGSSGTAISQASVVQTDPRTGKQVTTLIPLLGYDGPVNAVDFQEHATNWSFGALYK
                     LDPDLSVFARASHGTRFNADRTTNAGYFNPNGSLNAAGQANAAFPVDQYELGLKDRGD
                     LFSGHYTVELTSFYSTYKISSQEISITNCLAILGINEETCIISGKYQDAGIELFSTYR
                     VNGFNILFSGTYDDSKVQAHQGASYLRSPNIPNFTYTGLFSYDIISRAEVGISLDGQS
                     TITGGDGNTYPSSLIVGAFAKFEPVKNLQLGLNVYNLTNSFTAPSASTFVTGSTTLVN
                     SSPAQGVAVKVSARLKF"
     gene            complement(2794..4296)
                     /gene="gtfA"
                     /locus_tag="VGE92_01930"
     CDS             complement(2794..4296)
                     /gene="gtfA"
                     /locus_tag="VGE92_01930"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR03852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEY1312601.1"
                     /translation="MKNSVQLITYVDRFGGGGIADLREILSGPLAAAFGAVHLLPFFN
                     PIDGSDAGFDPIDHTQVDPRLGSWRDVQALSTELGVMADVIVNHISDRSAQFLDFCEN
                     GSASTYSGLFLTRDSVFPQGASAADLAAIYRPRPGQPFVELTLKNGQHVTLWSTFTRH
                     QLDIDVRHPLGKAYLDRVLGTLAAAGVSMVRLDAVGYAVKKPGTSCFMLPETFDFIGE
                     FAVRGRKLGVEVLVEVHSYFGRQIEIARLVDWVYDFALPPLVLHAFAFGTAVYLKHWI
                     RIRPRNAVTVLDTHDGIGIVDIGADPSDRKRRPGLVPPEQIDGLVEHIHAASGGQSRL
                     ATGAAASNLDLYQVNCTFFAAIGHDQRRYLLARAIQFFLPGIPQVYYVGLLAGSNDME
                     LLQRTGVGRDINRHYFDRAEITRELDRPVVKDLLALIRLRNEHAAFSGNFSLEESSAN
                     SLAMRWAAPGHFAELTVDLASAAFKLCYSSAAGSEEFTPTSAPPLTFRGE"
     gene            4491..5510
                     /locus_tag="VGE92_01935"
     CDS             4491..5510
                     /locus_tag="VGE92_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY1312602.1"
                     /translation="MAAPPDGKLQMEDIARLAGVSTSTVSRALNQSSLVNAETRARIE
                     QLARTLNYSVNVGASNLRLQRNRTVAVVLPLDSVTRQHVSDPFFVSLLGSIADALTAR
                     GYDMLLSRVDAERLDLAGELAASGRAIGVVLIGQWHHHDQINDLAAQRVPVVVWGAQL
                     PQQLYCTVGGDNIAGGVLATEHLLAKERRRIAFLGDIDLPEIGQRYEGYERALAQRNI
                     ANASQLLRRTPFIEGAARNAIHELLDRRIRFDGIFASSDMLALESISALRERGLKVPR
                     DVAVVGYDDIGIARYYHPSLTTIRQPVLKGGEAIVDALLKVIDGKKPKSQILPTELIV
                     RDSSG"
     gene            complement(5515..6756)
                     /locus_tag="VGE92_01940"
     CDS             complement(5515..6756)
                     /locus_tag="VGE92_01940"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1312603.1"
                     /translation="MRGAGRQNIALIRSLTFLMFMMFAMTTDSVGIIIPQIVKEFKLS
                     LTAAGAFHYATMTAIATAAILLGFLADRLGRKQTIVLGLTLFAVNSYLFIAGNSFAYF
                     LCLLVISGASIGIFKTGALALIGDISSSTAQHTSTMNLVEGFFGIGAIIGPFIVTALI
                     NAHVSWKWLYTIAGTVCVMLILVALAVRYPTTMKTSEEPVDLRRTLRMMKNPYALAFG
                     FAALLYVAVEASVYVWGPLYLADYRGRFTTLASYAVAVFFLLRAAGRFLGVWLLARFD
                     WGRVLALLSCAILLCIGGSAFGGTGVGVVLLPLSGLFMSVIYPTLNSKGISCFQKSEH
                     GAVAGVILFFTCAGAVLGPLAMARVIDVMGVPKYAFVLATVFAATLSAGLIFNWLRQP
                     TRRVLAALDAREYGVTAPVRT"
     gene            6897..7805
                     /locus_tag="VGE92_01945"
     CDS             6897..7805
                     /locus_tag="VGE92_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY1312604.1"
                     /translation="MSSAQQLFGAIEAGGTKFICAIGDAGGGILDETRIETRAPAATL
                     ADVLSYFSGAQHTFGRLTALGVGAFGPVDLRPNSPTFGFITSTPKPGWRNTDLAGVLR
                     RGLNLPVYIDTDVNGAALGELRWGSGQNLESLVYVTVGTGVGVGAVHNGRAVHGLMHP
                     ELGHMLVRRHPADSSFNGICPFHGDCLEGLACGAAIVARTGRALKDAPPEDPIWSIEA
                     DYLGQLCALLVLAHSPQRILMGGGVMQPQLYAGVRERMLYWLRNYIDAEELRSPYYLV
                     APGLGAAAGIKGALSLAMNGSAAIER"
     gene            7911..8483
                     /locus_tag="VGE92_01950"
     CDS             7911..8483
                     /locus_tag="VGE92_01950"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY1312605.1"
                     /translation="MVALVILVFILVAFVSYAVVLYNGLLRVAAGVKLAWSNIDVLLV
                     QRHDELPKLVEVCRQYMQYEGDTLERVIKARSAADSARSSGSVSSVGAAERELRSGLN
                     GLYAVAERYPDLKANEAFRQLQSRISGLETAIADRREIYNDSVNANNIRIQTFPDVLV
                     AQFGNFPPAKLLQFQSEEKADVDVKALFAR"
     gene            8497..>9006
                     /locus_tag="VGE92_01955"
     CDS             8497..>9006
                     /locus_tag="VGE92_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312606.1"
                     /translation="MPIATANSAQLHLLEAAALSIGAAWCLYKFAASVRRDRLLADTP
                     LVKIRSAAQGYVKVFGRAAPAGQAATAAPLSSRPCVWWSYDVAQKTRNAKGETTWKSI
                     DSATSVEPFLLRDADGECLVGPVSAEITPTSHNVWFGDLERPLGLPALAHTFIEQGDF
                     KYTERLLSVG"
BASE COUNT         1866 a         2679 c         2747 g         1714 t
ORIGIN      
        1 gcgtgtggcc cagggcggat gcagcatgat aagtaactgc acccaccctg ccaatcgggc
       61 ccgcgcgcgc ggcaagacac acgggtgagc tcgcgacctg ggctagaact tgagccttgc
      121 cgaaaccttc acagcaacac cctgcgcagg gctggaattg accagcgtag tgctcccggt
      181 gacgaaagtc gacgcacttg gggccgtgaa actgttggtc agattgtaca cgttgagccc
      241 gagttgcagg tttttgaccg gctcgaactt ggcgaacgca ccgacaatga gcgagctcgg
      301 ataagtgttg ccatcgccgc cggtaatggt cgattggccg tccaagctga tgccaacttc
      361 agctcggcta atgatgtcgt agctgaacag tcccgtgtaa gtgaagttcg gaatgttggg
      421 cgacctcaga tacgaagcgc cctggtgagc ctgcaccttc gagtcgtcat aggtgccgct
      481 gaacaggatg ttaaacccgt tgacccggta ggtcgaaaac aattcgatgc ccgcatcctg
      541 atatttcccc gagatgatgc aggtttcctc attgatgcca agaatggcca agcaattggt
      601 aatagaaatt tcctgagagg aaattttgta ggtcgaatag aaactcgtca gttccacggt
      661 gtagtgaccg ctgaacaggt cgccgcgatc tttcagaccc agctcatatt ggtccaccgg
      721 gaacgccgcg ttggcctgac cggcggcgtt gagcgagcca ttcggattga aatagcccgc
      781 attggtcgtg cggtctgcgt tgaagcgagt tccgtgcgac gcacgcgcga acaccgacag
      841 atcggggtcc agcttgtaca gcgccccgaa cgaccagttg gtcgcgtgtt cctggaagtc
      901 gacggcattc acggggccgt catagcccaa gaggggaatg agcgtcgtca cttgtttgcc
      961 cgtgcgcgga tccgtctgaa caaccgaggc ttggctgatc gcggtgcccg agctgccctg
     1021 cgcccaacca gaaccctgga agaactcttc acgaacgctg ccgtcgagat tcaagttgcc
     1081 cacgtcgacg ttgaggtcgg catagggtgc gttatccgtg aaggtgaggt cgaactgttg
     1141 gttccagcct tggttgaatc cgctcgtacc accggcagcc agcaggttgc cggtgccgtt
     1201 cagtccgctc accagatcaa gctcggccgg gtcgacgcca cttgcttcct ggagattgct
     1261 gtttgaatgc caatcttcag cgatgcgctg tgaatagtac aaataaccca ctttcacatc
     1321 gaaatgcgcc gggctcaaat cgccgctgta tgaggctgac agatcgttgg cgatatgatc
     1381 catatggctc atattggtgt gtacctgcgc gttattgttc acatacgcgc cggtaaatgc
     1441 ctggccagca ttgggcccat tggcgtagat cagcgactga cccgccccca acgcactcgc
     1501 agtgggtgtc gcattgtaga attgcacgtc gaacgcaccg ctcatccgcg cgacgcgcgc
     1561 attgtcgtca atcaccagtc cgccgccata gttataatgc gtctgcactt gcagccactt
     1621 ctcatcggtg aggatgccgt tcaacggctg tctggcgagg tcgccgctga cgttggagaa
     1681 cagggcgctc gagttcagcg cggaatagcc aggagcctgg ttgcgggcgt cgcaatacgg
     1741 ggatggcccg agattgctca cggtgtggcc ggtcaaagtc gcggagctga tgcagccgtt
     1801 atagctgggt tcctgcgtgt cagaggcttt gaacagcaaa cgcacatagc ccttatcgtc
     1861 ggccagttcc ttggtgatgt tgcccttgat cgcataggaa tcgctgacat tgtaggccgc
     1921 atgctcgatg ccacggccga catcgtagta accgccgata ttgtaatagg tcgtgtcatt
     1981 gatgctgctg ccgaggcgga aattgacctt agtaaagtcg tagttggcac ccttctcgag
     2041 ctcgacaaag ccgccgtcgt tgcgccccgt gaagctgata tggttcagca cggcaccgat
     2101 cgcctgggat gccaaggtcg cagcggtgcc gccgcgaatg gcttcgacgc gctcatcggt
     2161 cggcatcggg tgtgtccaat agtcgttgtt gccgaactgg atgtcaccga agagaaccac
     2221 gggaaggccg tcttcctgaa tctgcacgaa gggcgagccg cccgtcggag tgcgtagacc
     2281 gcgaactccg atgttcgagt tgccgccatc gccctgggta cccgaaacct ggataccagg
     2341 aatcatgcgg tagagctctg cggtgctggt cgcgtcaaaa ttctgaattt gctcttggtt
     2401 gatcgagctg attgaagtcg atgtgttcag tttcgactta tcgccggccg atgcggtgat
     2461 gacgatggtt tcgagcgtat cgcttgcatc cgccgcggcg ctatcgctag ttgccgccgc
     2521 cgtggttgca gtcgccgcag cgccagcctg agaaaacgcg ggcgccgaga caaccgccat
     2581 cgaggtcgcg gccagaatca tttgaatgct ctggcgcaac ttagcttgat ttggattcat
     2641 tagcgtccac cccttagacg taaaaaaaag atggtctctg cggaccgtgg tggaatttgt
     2701 agcatgatgt gcaaacgatt gcaacaaacg attgcaaacg gttttattcc agcaacatca
     2761 cggccgcgcc gcttgcctca agccaggtcg ggattactcg cctctgaagg tcagcggggg
     2821 cgccgaagtt ggcgtgaatt cttcgctgcc cgcggccgac gagtaacaaa gtttgaacgc
     2881 cgcgctcgcc aaatccaccg tcaattcggc gaagtgtccg ggagccgccc agcgcatcgc
     2941 aaggctgttc gcgctcgatt cctcgagcga aaagttgccg gaaaaagccg catgctcgtt
     3001 gcgcagacga atcagggcga gcagatcctt gacgacgggt cgatccagtt cccgtgttat
     3061 ttccgcgcgg tcgaaataat gacggtttat atcccgcccc actcccgtgc gttgcagcag
     3121 ttccatgtcg ttgctaccgg ccaacaagcc gacgtaatag acttgcggaa tacccgggag
     3181 aaagaactga atggcccgcg ccaacaggta gcgccgctga tcgtgcccaa ttgctgcgaa
     3241 gaaagtgcag ttcacctggt acagatcgag gttcgatgcc gccgcgccgg tcgccagccg
     3301 gctctgtccg ccgctggcgg catgaatgtg ctcgaccagc ccatcgattt gttccggcgg
     3361 taccaacccc gggcgccgct tgcgatcgct gggatccgca ccgatgtcta cgatcccgat
     3421 tccgtcgtgc gtgtcgagaa cggtcacggc attgcgcggc cgaatgcgaa tccagtgctt
     3481 aagatacacc gccgtgccaa aggcgaacgc gtgcaatacc agcggcggca gcgcaaaatc
     3541 gtacacccaa tccacgaggc gagcgatttc aatctggcgc ccgaagtacg agtgcacctc
     3601 gaccagcacc tcgacgccca gcttgcggcc gcggaccgcg aattcgccga tgaaatcgaa
     3661 ggtctccggc agcatgaagc aactcgtccc gggctttttg acggcgtaac cgacggcgtc
     3721 gagtcgtacc atgctgacgc cggccgcagc caaggtgccg agcactcgat cgagataagc
     3781 cttccccaac ggatgccgca catcgatgtc gagttgatgc cgggtgaagg tcgaccacaa
     3841 ggtcacgtgc tggccgttct tgagcgtgag ttcgacgaaa ggctggccgg gacgggggcg
     3901 ataaatagcg gccaaatccg ctgcgctcgc gccctgcggg aacaccgaat cgcgcgtcag
     3961 gaacaagccg ctgtatgttg aagcggaacc gttttcgcag aaatccagga attgcgcgga
     4021 acggtccgag atgtgattga cgatgacgtc ggccatgacg ccaagctcgg tcgacagcgc
     4081 ctggacgtcg cgccaactcc cgagccgcgg atcgacctgc gtgtgatcga tcggatcgaa
     4141 cccggcgtcg gagccatcga tcggattgaa gaacggcagc agatgcacgg cgccgaatgc
     4201 ggcggcgagc ggtcccgaga gtatttcacg taaatcagcg atgccgccgc cgccaaagcg
     4261 gtccacataa gtaatgagtt gtacgctatt tttcatcgcg gcgcgccgtg gcaggagcgc
     4321 tcaaagatag catcgcctgc aaacgattgc tattttaccc tgttgcgcgg atgcttggct
     4381 gcgcgcgagg cggcccgatt gctatgatga ggcgctgaag agccacccga acccgaatga
     4441 acaaagttcg ccgatccgca tccctcagga agcccgtcaa gcgcggcgcg gtggccgcgc
     4501 ctccggacgg caagctgcaa atggaagaca tcgcgcgcct cgccggggta tcgacatcga
     4561 cggtgtcgcg cgcgctcaat caaagctcgc tggtgaacgc cgaaacgcgc gccagaatcg
     4621 agcagcttgc gcgaaccctc aactattcgg taaacgtcgg cgccagcaat ctgcggctac
     4681 aacgcaatcg gacggtagcg gttgtgctgc cgctcgactc ggtcacccga caacacgtat
     4741 cggatccttt tttcgtcagc cttctcggca gcatcgccga cgcgctcact gcacgcggct
     4801 atgacatgct gttgtcgcgt gtggacgcgg agcgtctcga cctcgcaggc gaactcgcag
     4861 catcggggcg tgcgatcggc gtggtgttga tcggccaatg gcaccaccac gatcagatca
     4921 acgacttagc cgcgcagaga gtcccggtcg tcgtttgggg cgcacagctg ccgcaacaac
     4981 tctattgcac cgtcggcggc gacaatattg ccggcggcgt gttggcaacc gaacatctgc
     5041 tagccaagga gcgtcgccgc attgccttct tgggtgacat cgatctgccg gagataggcc
     5101 agcgctacga aggctatgag cgggctctcg cacagcgcaa tattgccaac gcgtcgcagc
     5161 tgttgcgccg cacgcccttc attgagggcg ccgcgagaaa cgcgatccat gagttgctcg
     5221 atagaaggat ccgattcgac ggaatttttg ccagcagcga catgctggcg ctcgaatcga
     5281 tcagcgcact gcgcgaacga gggctcaaag ttccgcgaga cgtggcggtg gttggctacg
     5341 acgacatcgg catcgctcgc tactaccatc cctcgttgac gaccatcagg caaccggtct
     5401 tgaaaggcgg cgaagccatc gtcgatgccc tgctcaaggt gatagacggc aaaaagccga
     5461 agtcgcagat tttgcccacc gaattgatcg tccgcgacag cagcgggtga aacctcaggt
     5521 gcgaaccggc gccgtaacgc cgtattcgcg cgcatcgagc gccgcgagca cgcgccgcgt
     5581 cggttgacgc agccagttga agatcagtcc cgcgctcagc gttgcggcga acaccgtagc
     5641 caatacgaac gcgtacttcg gcacccccat gacatcgatc acgcgggcca tcgccagcgg
     5701 accgagaacc gctccggcgc aagtgaagaa caagatcact ccggcgacgg cgccgtgctc
     5761 ggatttttga aagcagctga tcccttttga gttcaatgtc ggataaatca ccgacatgaa
     5821 cagacccgat aggggcaaca gcacgacgcc gacgccggtg ccgccgaaag cgctgccgcc
     5881 gatgcataac agaatggcac agctcaacag cgcaagcacc cgaccccaat cgaatcgggc
     5941 caacaaccag acaccgagaa agcgtcccgc ggctcgcagc aggaagaaga ccgcgaccgc
     6001 gtagctcgcc agcgtcgtaa agcggccgcg atagtccgcc aagtacaacg ggccccacac
     6061 atagaccgac gcctccacgg caacgtacag caaggcggca aatccgaacg ccagcgcata
     6121 ggggttcttc atcatccgca gggtacggcg cagatccacc ggctcctcgg aggtcttcat
     6181 ggtcgtcgga tagcggacgg caagcgccac cagaatgagc atcacgcaca ccgtgccggc
     6241 aatggtgtac agccatttcc acgacacgtg ggcgttgatc agggcggtga cgataaacgg
     6301 gccgatgatt gcgccgatgc cgaagaatcc ttcgaccagg ttcatggtgg aggtgtgctg
     6361 ggcggtcgac gacgatatat caccgatcaa cgccagcgcg ccggtcttga agataccgat
     6421 cgacgcgccc gagatgacca gcagacacag gaaataggcg aaggaattcc cggcgatgaa
     6481 caggtaggaa ttcaccgcga acaacgtgag cccgagcacg atggtctgtt tgcggccgag
     6541 gcggtccgcg aggaaaccca gtagaatcgc ggcggttgca atggcggtca tggtcgcgta
     6601 atgaaacgca cccgctgccg tcaggctgag cttaaactct ttgacaatct ggggaatgat
     6661 gatgccgacc gaatcggtgg tcatcgcaaa catcatgaac atcaggaacg taagggagcg
     6721 gatcaatgcg atgttctgcc gccctgcccc cctcatgttc gcagcgccct ccgcgtgttc
     6781 gcggttatga ctgtatcccc cggttcaaaa ccaatctatg atatcttaca atcgattgca
     6841 aggcggcgtt cgcacggcct cgacggccgg cacaacgcgc gcactggggg atcgcgatga
     6901 gcagcgcaca acagcttttc ggcgccatcg aagccggcgg caccaaattc atttgcgcga
     6961 tcggcgatgc cggcggcggg atcctggatg aaacgcgcat cgaaacgcgc gcgcccgcgg
     7021 ccaccttggc cgacgtgttg agttatttca gcggcgcaca acataccttc ggccggctca
     7081 ctgccctcgg tgtcggcgca ttcggtccgg tggatttacg gccgaactcg ccgacattcg
     7141 gtttcatcac aagtactccc aagcccggat ggcgcaatac ggatcttgcg ggcgtgctac
     7201 gccgcggact gaatctcccg gtgtatatcg atacggacgt gaatggcgcg gcgctcggcg
     7261 agttgcgctg gggctcgggg caaaatctcg agagcttggt ctacgtgacc gtgggcacgg
     7321 gcgtcggggt gggcgcggtt cataatggcc gcgcggttca tgggctgatg catcccgagc
     7381 tcggacatat gctcgtgcgg cgccatccgg cagattcgag tttcaacggg atctgtccct
     7441 tccacggaga ttgcctcgag ggtctcgcct gcggcgccgc gatcgttgcc cgaactgggc
     7501 gcgcactcaa ggacgcgcct cccgaggatc caatttggtc gatcgaggcg gactacttgg
     7561 gccagttgtg cgcactgctc gtcctcgctc actcgccgca acgcattttg atgggcggcg
     7621 gcgtcatgca gccgcagttg tatgcaggtg ttcgcgaaag aatgctctac tggctcagga
     7681 attacatcga tgcggaagaa ttacgcagcc cgtactacct ggtggcgccg ggacttggcg
     7741 cagcggcggg aatcaaaggt gcgctgtcgc tcgccatgaa cggcagcgca gcgatcgagc
     7801 gttaagtcac actccttgag ggcggcgctg cgaatttgcg gcgcgcactg ttagaatcgc
     7861 agcggctgca cggcttccgg gcggccgcga agttcacagg agtggaatgt atggtcgctt
     7921 tagtcatctt ggtgttcatc ctcgtcgcat tcgtaagcta tgcggttgtg ctctacaacg
     7981 gcctgctgcg cgtcgccgcc ggcgtcaagc tcgcgtggtc gaacatcgat gtcctcctgg
     8041 ttcaacgcca cgacgagttg ccgaagctcg tggaggtgtg ccggcaatac atgcagtacg
     8101 agggcgacac tctggaacgc gtgatcaaag cgcgcagcgc tgccgactcg gcacgcagct
     8161 ccggcagcgt gagttcggtc ggagccgcag agcgtgaact gcgctcgggg ctcaatgggc
     8221 tgtacgccgt tgccgagcgc tatcctgacc tcaaggcgaa cgaagcgttt cgccagttgc
     8281 agtcgcgcat ctcgggattg gagaccgcga tcgccgaccg ccgcgagatc tacaacgact
     8341 cggtcaacgc caacaacatc cgcatccaga cctttccgga tgtgctggtg gcgcaattcg
     8401 gaaacttccc gcctgccaag ctgctgcaat tccagagcga agagaaggca gatgtggacg
     8461 tgaaggcgct gttcgcgcgc tgacggccgg acgctcatgc cgattgccac ggcaaattct
     8521 gcgcagctcc acttgctcga agccgcggcg ctgtccatag gcgcagcctg gtgcctctat
     8581 aaattcgcgg cgagtgtgcg ccgcgaccgt ctgctggccg ataccccgct agtcaagatc
     8641 cgatccgcgg cgcaaggcta cgtcaaggtt ttcgggcgcg ctgcgcccgc cggccaggct
     8701 gcaacggcgg cgccgctcag ctcgcggccc tgcgtttggt ggagctacga tgtggcgcag
     8761 aagactcgca atgcaaaagg cgaaaccact tggaagtcga tcgacagcgc caccagtgtc
     8821 gaaccttttt tgctgcgcga tgccgatggc gaatgcttgg tcggacccgt cagcgcggag
     8881 ataacaccga ccagtcacaa tgtgtggttc ggggatctgg aacgtccgct gggcttgccg
     8941 gcgctcgcgc acacattcat cgagcaaggc gacttcaagt acaccgagcg gctgctgagc
     9001 gtcggc
//
LOCUS       DASWZT010000073         4530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_10275, whole genome shotgun sequence.
ACCESSION   DASWZT010000073 DASWZT010000000
VERSION     DASWZT010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10275"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="VGE92_01960"
     CDS             complement(<1..850)
                     /locus_tag="VGE92_01960"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1312607.1"
                     /translation="MFGGSAGNLVEWFDWYTYSAAALYFAPIFFPHGDRTGQLLQTAA
                     VFAVGFFARPVGAWLMGLYADRVGRKAALTLSVSMMCTGSLAIAVTPGAALIGVAAPL
                     LLLLARIFQGLSLGGEYGASATYISEMAGSRYRGFWTSFHYVTLIGGQLTALAVLIIL
                     QHVLSQADMAAWGWRIPFAIGAGLAIVAYWLRRQMQESQSFVRAAAHGPVRGNIRQIL
                     LEHPREILTVLGFTAGGSIIFYVYTTYMQKFLTNTAGFSRDQATEISALSLLAFMAAQ
                     PLCGWLS"
     gene            complement(912..1457)
                     /locus_tag="VGE92_01965"
     CDS             complement(912..1457)
                     /locus_tag="VGE92_01965"
                     /inference="COORDINATES: protein motif:HMM:NF023363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HEY1312608.1"
                     /translation="MRLNDSLLAMLARAALLSVAGCAAVAPVVGDIPAALRVADSALL
                     IRQTHGVGAQIYRCRTDKDDPTRFAWALKEPEAELFDRSGVKIGKHYAGPTWEARDGS
                     KVIGEVVARADSANSNAIPWLKLTAKSTSGPGIFASVRFIQRLHTVGGNAPSDGCNAG
                     AADREVRVSYSADYWFYADKP"
     gene            1518..1913
                     /locus_tag="VGE92_01970"
     CDS             1518..1913
                     /locus_tag="VGE92_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY1312609.1"
                     /translation="MYQGSCLCGRVKYEVRELGPIGLCHCSRCRKANGSAFLAAAQVK
                     PDDFKLISGQDSLGDFESSAGVHRIFCVHCGSPLYSRRPGPPEILRLRIGTLDTPLPG
                     KVQSHIFFADKAPWFEFQDAAPKFAQRPA"
     gene            1920..2342
                     /locus_tag="VGE92_01975"
     CDS             1920..2342
                     /locus_tag="VGE92_01975"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY1312610.1"
                     /translation="MNRSIAFRDDIPFIKDMGIEFISADGGRSVVSLNLLARHLNSWS
                     VAHGGVLMTLLDVAMAIAGRSLDSAVGGGITVEMKTSFLQPAKAGSRLIASAHAFHRS
                     STLVFCDGEVRDTGQRLIAKSLGTFKYRRVGAPENSDG"
     gene            2335..3267
                     /locus_tag="VGE92_01980"
     CDS             2335..3267
                     /locus_tag="VGE92_01980"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY1312611.1"
                     /translation="MADAQRLILHNYPNSPFAEKIRLIMGSKGMHWASVKIPTVMPKP
                     DVLALTGGYRKTPILQSGADIYCDSSLIAAVLDQLAPQPELYPAGIAAASRTLAQWAD
                     STLFWTAIPYALQPAGLTHVFAGVAPEAIKAFADDRKVFRANIPRMAGPEAEASLTLY
                     LERLEEMLGERSFFFGTRSSIADFSIYHCLWFVLRAGPQAQILESFPQLRAWRERMHA
                     FGHGACEELGSGAALAIAASAAAAPSLGMDFAVHGIAAGEKVAIAATDTGVEPSVGEL
                     YAATRERISIVRHDPRAGRVVVHFPRLGFELHRA"
     gene            3272..4435
                     /locus_tag="VGE92_01985"
     CDS             3272..4435
                     /locus_tag="VGE92_01985"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF005456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEY1312612.1"
                     /translation="MHWRSKLIHPRAHAPANFRSLATPTYRGSTVLFERQADVTDDWR
                     QTEKGYSYGLYGTPTALELAARIAEIEAAQHTFVVPGGQAAISLIYLAFCKTGAHALV
                     PNTAYGPNRALATGLMARFGVSVQSYDALLGADIARLIRDDTALIWLESPGSVTMEIQ
                     DVPAIVAAAHRHRVPVALDNTYSAGVLFDAFAHGVDVSMQALTKYVGGHSDLLLGTVS
                     VGGAAAYERIGSAYQQLGMAVSPDDCSLALRGLQTLGVRLERLESTALEVARWLQQHP
                     KIRTVLHPALESCPGHEHWRRDFSGSSSIFSIVFDERFTPDDVAACADRLQLFKIGYS
                     WGGVTSLAVTYPKLDRPGRDYGGRLLRLNVGLEEPADLIADLDQALQAIGSRV"
BASE COUNT          819 a         1484 c         1390 g          837 t
ORIGIN      
        1 ccgacagcca gccgcacagc ggctgcgcgg ccatgaaggc gagcagactg agcgcgctga
       61 tctccgtcgc ctggtccctg gaaaaacccg ccgtgttggt caagaacttc tgcatatacg
      121 tggtgtacac atagaaaatg atcgagccgc cggccgtgaa tccgagcacg gtcaagattt
      181 cgcgcggatg ttcgagcagg atctgccgga tatttccgcg cacgggcccg tgcgcggccg
      241 cgcgcacgaa ggattgggat tcttgcattt gccggcgcag ccaataggcg acgatcgcga
      301 gtcccgcgcc gatcgcaaac ggaatccgcc atccccacgc cgccatgtcg gcctggctca
      361 gcacatgctg caggatgatc aggaccgcga gcgcagtcag ctgcccgccg atcaatgtca
      421 cgtaatggaa gctcgtccaa aagccgcggt agcggctgcc cgccatctcg ctgatatagg
      481 tggcgctcgc gccgtattcg ccgccgagcg acaagccctg aaaaatgcgt gccagcagca
      541 gcagcagcgg agccgcgacg ccgatcagcg ccgcacccgg cgtcacggcg atggccagag
      601 atcccgtgca catcatgctg accgatagcg tgagcgcggc cttgcggccg acccggtcgg
      661 catacagtcc catcagccag gcgccgaccg gccgggcaaa aaatccgacc gcgaacaccg
      721 ccgcagtttg cagaagctgg ccggttcgat cgccatgggg gaagaagatc ggtgcaaaat
      781 acagcgccgc ggccgagtac gtataccagt cgaaccattc gaccagatta ccggccgaac
      841 cgccgaacac agcacggagt ctcgaaacct gcgccgctcc cgccggcggt gacgcgcctc
      901 gttgcacggt ttcagggctt gtcggcgtag aaccagtagt cggcggagta ggacacgcgc
      961 acctcgcgat cggccgcgcc cgcattgcag ccatccgacg gtgcattgcc gccgacggtg
     1021 tgcagccgct ggatgaatcg cacactcgcg aatatcccgg gacccgatgt cgacttcgca
     1081 gtcaacttga gccacgggat cgcgttcgaa ttcgcactat cggcacgggc aacgacctcc
     1141 ccgatcacct tgctcccgtc cctggcctcc caggtgggac ctgcataatg cttgccgatc
     1201 ttcacgccgg agcgatcgaa cagttccgcc tcgggttcct tcaaagccca ggcaaatcga
     1261 gtaggatcat ccttgtcggt gcggcagcgg taaatttgcg cgcccacgcc atgcgtctgc
     1321 cggatcaata gggcgctgtc ggcgacgcgc agggccgcgg gaatatcgcc gacgacggga
     1381 gccacggcgg cacatccggc cacgctcagc aacgcggccc gcgcaagcat cgccaacaat
     1441 gagtcattta gccgcacgct gcctccaaaa tcttgacgac gtatactaat ccatgccgat
     1501 actcatgagg gttttccatg taccaaggta gctgcctgtg cggaagggtc aagtacgagg
     1561 ttcgtgaact cggacccatc ggactttgtc attgctcgcg ctgtcgcaag gcgaacggca
     1621 gcgccttcct cgccgccgcg caagtcaagc cggatgattt caaattgatt tcgggccagg
     1681 acagcctcgg cgattttgag tcatccgccg gcgtgcatcg aatcttctgc gttcactgcg
     1741 gctcgccgct ctacagccgg cgtcccgggc ctccggaaat actgcggctg cgcatcggga
     1801 ctctcgacac ccccttgccg ggcaaggtgc aatcgcacat tttctttgcc gacaaggcgc
     1861 cgtggttcga gttccaggac gccgcgccga aattcgcgca gcggcccgcg taatcctgcg
     1921 tgaaccgatc gattgcgttt cgcgatgaca tcccgttcat caaggacatg ggcatcgagt
     1981 ttatcagcgc cgatggcggc cgctcggtgg tgtctctgaa tctgctcgca cgccacctga
     2041 atagttggtc ggtcgcacac ggcggcgtgc tcatgacgct gctggatgtc gcgatggcca
     2101 tcgcaggtcg ttctctcgac tccgccgtcg gcggcggcat caccgtggaa atgaagacca
     2161 gcttcttgca accggccaag gcgggatcgc ggctcatcgc ctccgcccat gcatttcatc
     2221 gctcgagtac gctggttttc tgcgacggcg aagtgcgcga taccggccag cgcctcatcg
     2281 ccaaatcgct cggaaccttc aagtatcgaa gagtgggggc gccggagaat tccgatggct
     2341 gatgcgcagc ggctcatttt gcataattac ccgaattcgc ccttcgcgga gaagattcgg
     2401 ctcatcatgg gttcgaaagg catgcattgg gcgtccgtca agatcccgac cgtcatgccc
     2461 aagcccgacg tgctcgcgct cacgggcggg tatcgtaaaa ctccgatcct gcaaagcggc
     2521 gcggacatct actgcgactc ctcattgatt gccgccgtgc tcgaccagct cgcgccgcag
     2581 cccgaacttt atccggccgg aatcgccgcg gcatcgcgca cgctggcgca atgggccgat
     2641 tcgacgctct tctggacggc gattccgtac gccttgcagc ccgccggcct cacgcacgta
     2701 ttcgcgggcg tcgcccccga ggccatcaag gccttcgccg acgatcgcaa ggtgttccgc
     2761 gcgaatatcc cgagaatggc cggcccggag gcagaggcat ctctcacgct gtatctcgag
     2821 cgtctcgaag agatgctcgg cgagcgcagc tttttcttcg gtacacgctc gagcatcgcg
     2881 gatttttcca tttatcactg cctgtggttc gttttgcgcg cggggccgca ggcgcagatc
     2941 ctggagtcct tcccgcagtt gcgggcgtgg cgcgagcgga tgcacgcatt cggccacggc
     3001 gcctgcgagg aactcggcag cggcgctgcc ctcgcgatcg ccgccagcgc cgcggcggcg
     3061 ccctcgctcg gcatggactt cgcggttcac ggcatcgccg caggggagaa agtcgccatc
     3121 gccgccaccg ataccggagt cgaacccagc gtcggggagc tgtacgccgc gacccgcgag
     3181 cgcatctcga tcgtgcggca tgatccgcgc gccgggcgcg tggtggtgca tttcccgcgc
     3241 ttgggattcg agctgcaccg ggcgtaacga gatgcattgg cgctccaaac tcatccatcc
     3301 ccgcgcccat gcacctgcca acttccgctc tctggcgacg ccgacgtatc gcggttcgac
     3361 ggtcttgttc gaacgccaag ccgacgtcac ggacgactgg cgccagacgg aaaaaggcta
     3421 cagctacggc ctgtacggca ccccgacggc gctcgagctg gccgcgcgaa tcgcggagat
     3481 cgaagcagca cagcacacct ttgtagtgcc gggaggtcag gcggcgatct cgctcatcta
     3541 cctggccttc tgtaaaaccg gggcgcatgc cctcgtcccg aacacggctt atggaccgaa
     3601 tcgcgcgttg gccacgggac tcatggcgcg attcggcgtc tcggttcaat cgtatgacgc
     3661 gttgctcggc gcggatatcg ctcgactgat ccgcgatgac acggcgctca tttggctgga
     3721 gagtccggga tccgtgacca tggaaattca agacgtgccg gctattgtcg cggcggccca
     3781 tcgccaccgg gtgccggtgg cgctcgacaa tacctactct gccggagttc tgttcgacgc
     3841 ctttgcgcac ggtgtcgatg tcagcatgca ggcattgacc aaatatgtcg gcggccacag
     3901 cgacttgctg ctcggcaccg tatccgtcgg cggcgccgcc gcctacgagc ggatcgggtc
     3961 ggcttaccag cagctcggaa tggccgtatc tcccgacgat tgcagcctgg cgctgcgcgg
     4021 cctgcaaacc ctgggcgttc gcttggaacg gctcgagagc acggcgctcg aggtggcgcg
     4081 ctggctgcag cagcatccca agatccgcac agtgttgcat ccggcgctcg aatcttgtcc
     4141 cggtcacgag cattggcggc gcgatttcag cggttcctcg agcattttct cgatcgtctt
     4201 cgatgagcga tttacgcccg acgatgtcgc cgcctgcgcg gaccgactgc agttgttcaa
     4261 gatcggctat agctggggtg gcgtgaccag tctcgccgtg acctatccca agctcgatcg
     4321 tccggggcgg gactatggcg gccggctgct gcggctcaac gtcggtctgg aggaacccgc
     4381 ggatttgatc gccgatctcg accaggcctt gcaggccatc ggttcacggg tctgacggaa
     4441 aacaatgtca atcaacatgg cattcgtgct gcgaatcgtg gccatctcgc tggcgacgtt
     4501 catcgtcacg atttcggcgc tttggggcgc
//
LOCUS       DASWZT010000074         3275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_58629, whole genome shotgun sequence.
ACCESSION   DASWZT010000074 DASWZT010000000
VERSION     DASWZT010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58629"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VGE92_01990"
     CDS             complement(<1..593)
                     /locus_tag="VGE92_01990"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF013628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEY1312613.1"
                     /translation="MLRMSQSPALANWHPASWHARPATQQPKYLDPVVLERVVAALSR
                     LPPLVVSWEIEALRAKLAQAQRGEQFLLQGGDCAESFEDCESDNIARRLKILLQMSLV
                     LLQGLKKPIIRVGRFAGQYAKPRSAETETRDGVTLPCYRGDNVNRPGFSAAERNPDPE
                     LLLRGYERASLTLNFVRALIDGGFADLHHPEYWDLGFL"
     gene            667..2193
                     /locus_tag="VGE92_01995"
     CDS             667..2193
                     /locus_tag="VGE92_01995"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY1312614.1"
                     /translation="MQTPDPLQALGLRDMNAGSWSGTAGWSKQSSGPVIDSMNPATGK
                     RLAQVRGATAQDYEQVMTAAVAAAAAWRRVPAPKRGEAVRLVGEELRRCKDALGSLVA
                     MENGKIKAEGDGEVQEMIDIADFAVGQSRMLYGLTMHSERPQHRMYEQWHPLGVVGVI
                     SAFNFPVAVWSWNAFLAAISGNATVWKPSPKTALCSLAVQTICNRVLERHSLPPIFQI
                     FIDAGTELAMRFVADRRVALVSFTGSTHVGRQIGVIVAERLGKSLLELGGNNAIIVDD
                     TANLDLAVPGIVFGAVGTAGQRCTTTRRVLVHRSRAAELERRLIAAYGQVRIGDPLEP
                     GTLMGPLIDGAAVARYSAAIAAAQAQGGELMYGGKVLARPGNFVEPTIIRANAGMAVV
                     GTETFAPILYVIAYDSLDEAVAIQNSVPYGLSSAVFTDRLQVAERFLSAEGSDCGIAN
                     VNLGTSGAEIGGAFGGEKDTGGGREAGSDAWKAYMRRQTNTINWSSQLPLAQGIRFNV
                     "
     gene            complement(2194..>3275)
                     /locus_tag="VGE92_02000"
     CDS             complement(2194..>3275)
                     /locus_tag="VGE92_02000"
                     /inference="COORDINATES: protein motif:HMM:NF023326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEY1312615.1"
                     /translation="LTAWLKRERGMPDALLQFEPGAVPAHLHPRLSVTVDGKEFAHGQ
                     TLAELRRSSAVAARAELARRARASYPLAWRRFEIEELPQSLPLPLDRGTAWVYPSLAG
                     GSLGLEVRYEWSAAEAERSWRQGAALLARAMLAAQERDLRRAIAGNSALLLSASPYLG
                     SSELLETLSQWVFRRACFEDAAAPRTRAAFESAVDQGRARLHPAYEEIAASALNWFGE
                     ARAVRAALDAARALLAADADESHAHLGRLLGADALQAMSPDWLRQLPRYLRAEERRWQ
                     RRAARGLEPPRIVRELRDWAQRYQNLARAVGAELRWIPALDELRNWIEEYRVSLYAQE
                     LKTLGPVSAARLAERAAEIDAWLAR"
BASE COUNT          562 a         1099 c         1090 g          524 t
ORIGIN      
        1 aggaagccga gatcccaata ctcggggtga tgcagatcgg caaatccgcc gtcgatcagg
       61 gcgcgcacga aattcaacgt gagcgaggcc cgctcgtagc cgcgcaacag cagctccgga
      121 tccgggttgc gctcggcggc gctgaatccg ggtcgattca cattgtcgcc gcgataacac
      181 ggcagcgtca cgccgtcgcg cgtttcggtc tcggcggatc gcggcttcgc atactgaccg
      241 gcaaatcggc cgacgcgaat aatcggcttc ttcaaaccct gcaacagcac caggctcatc
      301 tgcagcagga tcttcaaacg ccgggcaatg ttgtcggact cgcaatcctc gaagctctcg
      361 gcgcagtccc caccctgcag cagaaactgc tcgccgcgct gggcctgggc caacttcgcg
      421 cgcagcgcct cgatttccca ggaaaccacc agcggcggca gccggctcaa tgcggcaacc
      481 acgcgctcga gcacgaccgg atcgaggtat ttgggctgct gggttgcggg acgagcgtgc
      541 caactggccg ggtgccagtt cgccagggcg ggactttgcg acatcctgag catgctagcg
      601 ttgtccgtcg gcccaagtca aaatgcggcc cctcggcata ccggccaaaa tcttctggaa
      661 ttacctatgc aaacacccga tcccctgcag gcactcggac ttcgcgacat gaacgccggc
      721 agctggtccg gcacggcggg ctggagcaaa caaagcagcg gccccgtgat cgattccatg
      781 aaccccgcca caggcaagcg tctggcgcag gttcgcggcg caaccgcgca ggactacgaa
      841 caggtgatga cggcggccgt ggctgcggcc gccgcgtggc gtcgcgtgcc ggcgcccaaa
      901 cgcggcgagg ccgtgcggct tgtcggcgag gagttgcgcc gctgcaagga cgctctcggc
      961 agcctcgtcg ccatggagaa cggcaagatc aaagccgaag gcgatggcga ggtgcaggag
     1021 atgatcgata tcgcggattt cgcggtggga caatcccgca tgttgtacgg cttgacgatg
     1081 cactccgagc gcccacagca ccgcatgtac gagcagtggc atccgctcgg cgtcgtgggc
     1141 gtgatctcgg cgttcaattt cccggttgcg gtctggtcgt ggaacgcgtt cttggccgcg
     1201 atcagcggca acgcgacggt gtggaaaccg tcgccgaaaa ccgcgctgtg ttcgctcgcc
     1261 gtgcagacca tctgcaaccg ggtgttggag cgccattcgc tgccgccgat ctttcagatc
     1321 tttatcgacg ccggcaccga gcttgcgatg cgtttcgtcg ctgaccggcg cgtggcactg
     1381 gtctccttta ccggctcgac ccacgtcgga cggcaaatcg gggtgatcgt ggccgagcgg
     1441 ctcggcaaga gtctgttgga gttgggcggc aacaacgcca tcatcgtcga cgacacggca
     1501 aatctcgacc tcgcggtgcc cggcatcgtc ttcggcgccg tcggcaccgc gggccaacgc
     1561 tgcaccacga cgcggcgcgt gctggtgcat cgctcacgcg ctgccgagct cgagcggcgg
     1621 cttatcgcgg cctacggaca ggtgcgcatc ggcgacccgc tggagccagg caccttgatg
     1681 ggtcccttga tcgatggcgc agcggtcgcg cgctactcgg cggccatcgc tgccgcacag
     1741 gcacagggtg gtgaactgat gtacggcggc aaggtgttgg cgcggcccgg caatttcgtc
     1801 gaaccgacca tcattcgcgc caatgccggc atggcggtgg tgggcacgga aaccttcgca
     1861 ccgatcttgt atgtgatcgc ctacgacagc ctcgatgagg ccgtcgcgat tcagaactcc
     1921 gtgccgtacg gtctctcgtc ggccgtgttc acggatcgat tgcaggtggc ggagcgcttc
     1981 ttgtccgcag agggcagcga ttgcggcatc gccaatgtca atctcggcac gtcgggcgca
     2041 gaaatcggcg gcgcattcgg cggcgagaaa gacacgggcg gcggccgcga agcaggctcg
     2101 gatgcctgga aggcgtatat gcggcgccag acgaatacca tcaattggag ttcgcagctg
     2161 ccgctcgcgc aaggaatacg cttcaacgtg tgatcagcgc gccagccatg cgtcgatctc
     2221 ggcggcacgc tctgccaaac gcgccgccga gaccggaccc agagtcttga gttcctgcgc
     2281 gtacagcgac acgcggtatt cctcgatcca atttcgcaat tcgtcgagcg cgggaatcca
     2341 acgcaattcg gcgccgaccg cgcgcgccag gttctggtaa cgctgcgccc agtcgcgcaa
     2401 ctcgcgcacg atgcgcggcg gctcgaggcc gcgagccgcg cgacgttgcc agcgccgctc
     2461 ctcggcccgc agatagcgcg gcaattgacg cagccagtcg ggcgacatgg cttgcagcgc
     2521 gtcggcaccg agcagccggc cgagatgcgc gtggctttcg tcggcgtcgg ccgccaagag
     2581 cgcacgcgcg gcgtcgaggg cggcacgcac cgcccgcgcc tcgccgaacc agttcaatgc
     2641 gctggccgcg atctcctcgt aagccgggtg cagccgagcg cgcccttgat cgacggcgct
     2701 ctcgaacgcc gcgcgcgtgc gcggcgctgc ggcatcctcg aagcaggcgc ggcgaaacac
     2761 ccactgcgac aaggtctcga gcagctcact gctcccgagg tacgggcttg cggacagcaa
     2821 cagcgcgctg ttgccggcga tcgcccgccg caagtcgcgc tcctgggctg cgagcatggc
     2881 gcgcgccagg agtgcggctc cctgccgcca actgcgctcg gcttcggcgg cggaccactc
     2941 ataacgcact tcgagtccaa ggctgccccc ggccaggctc gggtacaccc acgccgtgcc
     3001 gcggtcgagc ggcagcggca gcgactgcgg cagttcttcg atctcaaagc ggcgccacgc
     3061 gagcgggtag ctcgctcggg cgcggcgcgc gagttccgcg cgcgccgcca ccgcactcga
     3121 gcggcgcaac tccgccagtg tctggccatg cgcgaattcc ttgccgtcga ccgtcacgct
     3181 aaggcgcggg tgcaagtgtg cgggcacggc gcccggctcg aattgcagca gcgcatcggg
     3241 catgccgcgc tcgcgcttga gccaggcagt cagcg
//
LOCUS       DASWZT010000075         2160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_48810, whole genome shotgun sequence.
ACCESSION   DASWZT010000075 DASWZT010000000
VERSION     DASWZT010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48810"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VGE92_02005"
     CDS             complement(<1..463)
                     /locus_tag="VGE92_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312616.1"
                     /translation="MSPAGDNDGIPLCVNCEFALLKANVVHEAVLLLFRKSLRSLFML
                     PGWVLRGPAELTRRLSGEVSVNWRNMPVNEGVVQCMRAALAANRPVFVLTSMPRSWAS
                     QLCAELGFPQATVVDANGAALADACGARGFEYIGDGRRDLPLCRAARSVLLV"
     gene            complement(698..>2160)
                     /locus_tag="VGE92_02010"
     CDS             complement(698..>2160)
                     /locus_tag="VGE92_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312617.1"
                     /translation="TCVSSSGTPVAGSGGCPAGAAVSNQSSFVSALRSSLSGQRVLFK
                     CGDTFTGDNAVVQGITFSVGAYGSCVGTTSGRPIFSDSGSTGEISIGSGSGATYDGRI
                     SDLDLEGNSSGYVGVAAGGSYNYYQITLNNLLSHGNTTNYDYYNDTQWGLIEVVASGQ
                     GATVGTFINDSENQCVNGNQSYNCGGTPNFVNVSYNAVMGGRFDGTGAPNNGHGIETF
                     RVSACRLCVFANNTFENANNIGATFKLHSGNPNSQNQWIGQYTELVEISDNLFTGTSG
                     AQLVEIAPQNNETDERLRNIVFERNLVYGIAGNSNKLLVSVHNGSFRNNAFNGTVTSG
                     VDYGIQIAQRGSEYTPSANFPCSNAVGVCPGSAPVYIEVYNNTCYVKGGNGCIGFDGA
                     NFIAPANSSWAANNMMYNSSGGTTVRDSGSGNAVSNNTASAASDPGFADVSGTYKFLS
                     DYLPTANYAGGISVANFFDALGEAWSPTWDLGAVHH"
BASE COUNT          406 a          672 c          667 g          415 t
ORIGIN      
        1 tgacgagcag gacgctgcga gccgcccggc agagcggcag atcccgtcgc ccgtccccga
       61 tgtactcgaa tccccgcgcg ccgcaggcat cggccagggc tgcgccgttg gcgtcgacga
      121 cggtggcttg cgggaaaccg agttcggcgc aaagctggga tgcccagctc cgcggcatcg
      181 aggtcagaac gaagaccggg cggtttgcgg cgagcgcggc ccgcatgcat tgcacgacgc
      241 cctcgttgac gggcatgttg cgccaattga cgctgacctc gccggacagc cgccgcgtca
      301 gctctgccgg cccgcgcagg acccagcccg gcagcatgaa caggctgcgc aggctcttcc
      361 tgaacagcag cagcacggcc tcgtgaacaa cattcgcctt cagcagcgcg aattcacagt
      421 tcacgcacag tggaatcccg tcattatccc ccgcggggct catgcgcgtg ccggcaatgg
      481 caatggaaca accggcgcaa gacgggattg cggatgcact aatgtccttt catgcgcacc
      541 aggaaggcaa aaatattagc agagtcaatg gcaagaagat attcagccgt cacatcgcgc
      601 gacggcgctg cgggcagcga aaccggctac gcggcgggcg atcgtattgc ggggattgca
      661 cgcatcgggg tcgctgtatg cgacgccccg attccgctca atggtgcacg gctcccaggt
      721 cccaggtcgg cgaccatgcc tcgccaagag catcgaagaa gtttgccacg ctgatgccgc
      781 cggcatagtt cgccgtcggc aggtagtccg aaaggaactt gtaggtgccg ctcacgtcgg
      841 caaatccggg atcgctggcc gcggaggcgg tgttgttgct gaccgcgttt ccggagcccg
      901 agtcacggac cgtcgtgccg ccggaggagt tgtacatcat gttgttcgcg gcccagctgc
      961 tgttcgcggg tgcgatgaaa ttcgcgccat cgaatccaat gcagccattg ccgcccttca
     1021 cgtagcaggt gttgttatag acctcgatgt acacgggcgc agatcccggg cagacaccca
     1081 ctgcattgct gcaggggaaa ttcgcgctgg gcgtgtattc ggaaccgcgc tgcgcgatct
     1141 ggatgccgta atcaactccg gaggtcacgg tgccgttgaa ggcgttattg cggaagctgc
     1201 cgttgtgtac cgataccagc agcttgttcg agttgccggc tattccatac accagattgc
     1261 gctcgaacac gatgttgcgc agccgttcat cggtctcatt gttctgcgga gcgatttcga
     1321 cgagctgcgc gcccgaagtt cccgtgaaca ggttgtcgga gatttcgacg agttccgtgt
     1381 attggccgat ccactgattc tgcgagtttg gattgccgct atgcaatttg aacgtcgcgc
     1441 cgatattgtt ggcattctca aacgtattgt tcgcgaacac gcacagccgg caggccgaaa
     1501 cgcggaaggt ttcgatgcca tggccgttat tcggtgcgcc cgtgccatcg aaccggccgc
     1561 ccatgacggc gttgtagctc acgttgacga agttcggcgt accaccgcag ttgtacgact
     1621 ggtttccatt cacgcactgg ttctcggagt cattgatgaa cgtgccgacg gtcgcgccct
     1681 ggccgctcgc gacgacctct atcaggcccc attgcgtgtc gttgtaatag tcgtagttcg
     1741 tcgtattccc gtgcgacagc agattgttca gcgtgatctg gtaatagttg tagctgccgc
     1801 cggccgccac gccgacatag ccgctcgaat tcccctccag atcgaggtcc gagatcctgc
     1861 cgtcgtaagt ggcaccggaa ccggaaccga tcgagatttc cccggtggat ccgctgtcgc
     1921 tgaagatggg gcgccccgac gtcgtgccga cgcagctccc gtaggccccg acactgaagg
     1981 taattccctg cacaaccgcg ttatcgccgg taaaggtgtc gccgcacttg aacaggacgc
     2041 gctgcccgct cagcgacgag cgcagtgccg agacaaagct cgactgattc gacaccgccg
     2101 ctcccgccgg acacccgcca ctccccgcga cgggcgtgcc tgacgaggat acgcaggtgg
//
LOCUS       DASWZT010000076         8681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_34411, whole genome shotgun sequence.
ACCESSION   DASWZT010000076 DASWZT010000000
VERSION     DASWZT010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8681
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34411"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="VGE92_02015"
     CDS             <1..1293
                     /locus_tag="VGE92_02015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1312618.1"
                     /translation="VQDLIAGTRAVAKGNYDTRLPLPGRDELGFLVTSFNDMTKRLGR
                     AREETRRSQQAVEAERAGLAVILARLSTGVVSLEADLTVKTANQAASAILGVDLEAAV
                     GKRFDESISDTTLFTQFLAVLKAHLGAEDLDWREQVELLSDSGKRVLMCACTALPNDS
                     GGAPGVVLVFDDITTLLQAQRDAAWGEVARRLAHEIKNPLTPIQLSAERMRRKFLGSM
                     NAQDAQILERATHTIVAQVDAMKQMVNAFSEYARAPDMNFSRFDVNQLITEVVDLYRV
                     QDPSADLKLLLNPLLPAISADRMRIRQILNNLVTNSLEALEGRPGACIEIQTLLAEEG
                     PKQAASVAIVVSDNGPGFPRDLIGTVFDPYVTSKPKGTGLGLAIVKKIVEEHGGRIEA
                     DNQRSGGARVRILLPLGDGARSLGVREPRKGEHRRERV"
     gene            1290..2651
                     /locus_tag="VGE92_02020"
     CDS             1290..2651
                     /locus_tag="VGE92_02020"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY1312619.1"
                     /translation="MTASRILVVDDEADIRGLLKEILSEEGYDVEVAADATQARSSRV
                     TQVPDLVLLDIWMPDTDGITLLREWSVTDGYDCPVVMMSGHGTVETAVEATRLGAFDF
                     VEKPLSLTKLLRTVERALDAGRRKRLSARTHGPTLAVPVGKSKVTQALREQVQQAATS
                     SSPVLLLGELGSGREAYARFLHSLSARATKPFFMVVAASLGSDPHSQLFGSERDGRVE
                     LGALDQAAGGSLYLNGLEDLNSEAQRALAGAIEQNGYTRVGGRQRVPLNVRWISSAQD
                     TFELRASPEPFRRDLVSHLNVITLRVPPLRDYAEDVPDLLRYYVDRLVDDQHLPLRRF
                     SVAAQNRLRNYPWPGNVGELKSLVQRVLVLGVGEEIRLEEIERELAVKLAVDEPLVKQ
                     DLLALPLREAREQFERAYLQQQLLLCNGKVGQLAKRVGMERTHLYRKLRALGVDFRQL
                     GDD"
     gene            complement(2667..3233)
                     /locus_tag="VGE92_02025"
     CDS             complement(2667..3233)
                     /locus_tag="VGE92_02025"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY1312620.1"
                     /translation="MARDYKQRRGKATGFSGWTGVVWGLAAGLSVAGIVYIKDHRPDA
                     PIARAGKVDKKKFRGSEFPDGETADSSAEEPAKSYDFYDMLPKFEVVVPEKDKDVRPD
                     TKSVPETRRGTYVLQAGSYKNFADADRVRAQLALQGVESKVQKVSVDNDTWHRIRIGP
                     ISKLDELNRLRQILRKADVDVLVIRVGD"
     gene            complement(3248..5008)
                     /gene="argS"
                     /locus_tag="VGE92_02030"
     CDS             complement(3248..5008)
                     /gene="argS"
                     /locus_tag="VGE92_02030"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY1312621.1"
                     /translation="MKTLIENLLRQALAAVPEDVVPMDARGVAVEVETTRDAQHGDFA
                     SNLAMRLAKATRQNPRNLAQLLLRSLPPCPALAKVDIAGAGFINFFLAKDAYHGEIAK
                     VLEEARRYGRSSAGAGRSVQVEFVSANPTGPLHVGHGRHAAYGASVANLLEAVGYRVQ
                     REYYINDAGRQMEILAASTWLRYLERCGEQFPFPANGYRGDYITAIGAELYAARGEQL
                     RRSAAQVFRDLPPDAPQGGDKDVYIDALISRARSLIGDAAWRRVLDLALAGILGDIRE
                     DLQEFGVSFDCWFSERSLSVDGSIDRAIAELKRAGHAYVKDGALWFKSTDYGDEKDRV
                     MVRDNGVKTYFASDIAYVFNKLERGFDHLLYIWGADHHGYITRLRAGLTALGGPAESF
                     EVRLVQFVSLFRGGEKAQMSTRSGEFVTLRALRNEVGNDAARLFYVMRSNDQHLDFDL
                     ELATSRSNDNPVYYIQYAHARVASVMRQMQERGLTHDPAHGLANLSALVEPQELQLIK
                     RVSAYPEIVRQCAQLRAPHTLVYYLRDLANDFHTDYSAHQFIVEDAALRNARIDLALA
                     TQTVIRNGLELLGVSAPDTM"
     gene            complement(5069..7276)
                     /locus_tag="VGE92_02035"
     CDS             complement(5069..7276)
                     /locus_tag="VGE92_02035"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004067.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEY1312622.1"
                     /translation="MILQIALDTPLRRVFDYRPPASLTGPGAAQRLRPGLRVRVPFGR
                     RQLIGILVGVAADSAVPLPKLKTAVEILDERPIVDPVTFDLLCWAAEYYHHPLGEVFA
                     AALPASLRAGQPALPPAECWSLTEAGREELSAPTARRAPQQRALLAWLGHRHSATTED
                     IRATFELRLLRALAARGWTAAQRAVPHAAPTHGATVSLPSPVELNQHQADCVAAVLAT
                     LPGFAVHLLYGVTGSGKTEVYLRAIAAAIADGAQALVLVPEIALTPQLVDRFRSRFSS
                     GVVALHSGLTGGERRDAWRSAHNGEARIVIGTRSAVFTSLPKLALIVVDEEHDMSYKQ
                     HEGFRYSARDLAVVRARRAGVPVILGSATPSLETLENAASGRYMKHLLPQRPGAVQAP
                     RMSLLDLRKCASDQGLSTPAMLAMAQHLKAGGQVIVFLNRRGYAPSLFCNSCGWVASC
                     AHCDARMTLHRRAQQLRCHHCGAHEAVPVICGSCGQPLHPIGQGTERVEETLSRLFPE
                     APLARLDRDTAAARGAVQDVLDRVHSGAARILVGTQMLTKGHHFPEVSLVVILDADQG
                     LFASDFRATERLAQTITQVAGRAGRGTLAGEVLIQTEFPEHPLLQRLMLHGYEGFAQS
                     ALQERRDAGWPPYSRLAMLRAEAKDLQGLDAFLRAAAQCASASQETAVKVLGPASALI
                     ARRADHFRAHLLIETSARATLQRFLGDWLPRVESLAGPPGLRWSIDVDPLEVD"
     gene            7593..8210
                     /locus_tag="VGE92_02040"
     CDS             7593..8210
                     /locus_tag="VGE92_02040"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF006550.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY1312623.1"
                     /translation="MPRFDTLNSAWMVWDNPAPAPEDAVLEYTRELEKFLAEVERRAF
                     RMAQVALRDPDDAMDVVQDTMLKLARNYASRPSAEWRPLFYRILENGIRDLQRRRSVR
                     NRFMTWLPGAKQDPDNEAQDPLETVADTSAPIPEELMRDQAMQQLEVSLRGLPARQRE
                     AFMLRNFEGLDVAETATAMGCSEGSVKTHYSRAVHTLREQLGEVW"
     gene            8210..8626
                     /locus_tag="VGE92_02045"
     CDS             8210..8626
                     /locus_tag="VGE92_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312624.1"
                     /translation="MNEQLPVQRRDALEERSRRLFLESVEGMDFATRSRLRQARTAAL
                     AAASTRRPWFSRAGVLAPAGVTAAAVLGAFLWLGSPLGPHAVTVVDGQSNFEDLELVA
                     ASDEGSGDALDMLQDDIEFYDWADKAAANSGPEAQS"
BASE COUNT         1592 a         2679 c         2874 g         1536 t
ORIGIN      
        1 gtgcaggacc tgatcgccgg cacgcgggcg gttgcgaaag gcaattacga cacccggctg
       61 ccgctgccgg ggcgcgacga actcggcttt ctggtgacct ctttcaacga tatgaccaag
      121 cgcttgggcc gggcccgcga ggagacgcgg cgcagccagc aggcggtgga agccgagcgc
      181 gcaggtcttg cggtcatcct cgcccgcttg tccacgggtg tggtgtcgct cgaggcggat
      241 ttgaccgtga aaaccgcgaa tcaggcggcg agcgcgattc tcggagtcga tttggaggcc
      301 gccgtcggca aacgattcga cgagtccatc agcgacacga ctctgttcac gcagtttctg
      361 gcggtgctca aagcgcatct cggcgccgag gacttggact ggcgcgagca ggtcgagtta
      421 ttgtcggact ccggcaagcg tgtcctcatg tgcgcttgca cggcattgcc gaacgactcg
      481 gggggggcac ccggcgtggt gctggtgttc gatgacatca cgacgctgct gcaggcgcag
      541 cgcgatgcgg cgtggggcga agtggcgcgc cggctggcgc acgagatcaa gaatcccctg
      601 acgccgatcc agctctcggc agagcgcatg cgccgcaagt tcctgggcag catgaacgca
      661 caagacgccc agatactcga acgggcgacc cacaccatcg tcgcccaggt tgacgcgatg
      721 aagcagatgg tcaacgcgtt cagcgaatat gcgcgcgcgc cggatatgaa tttctcgcgc
      781 ttcgatgtga accaattgat cacggaagtc gtggatctgt atcgagtcca ggatccgagc
      841 gccgacctca aactgctgct gaacccgctg ctcccggcca tctcggcgga ccggatgcga
      901 atccgccaga tcctcaacaa tctggtcacc aattcgctcg aggccctgga gggccggccc
      961 ggtgcctgta tcgagattca aaccctgctg gccgaggaag gccccaagca agccgcgtcg
     1021 gtggcaatcg tggtcagcga caatgggccg gggtttccgc gcgacctgat cgggacggtg
     1081 tttgaccctt atgtcacaag caagccgaaa ggcacgggtt tggggctcgc aatcgtcaag
     1141 aagatcgtcg aagaacacgg cggacggatt gaggcggata atcaacgcag cggcggggcc
     1201 cgcgtgcgaa ttctattgcc gctgggcgac ggcgcacgct cgctgggcgt gcgcgagccc
     1261 cgcaaaggcg aacacaggag agagcgagta tgaccgcgtc gagaatactg gtggtggacg
     1321 acgaggcgga tatccgcgga ttgctcaaag aaattctttc cgaagagggt tatgacgtcg
     1381 aggtggcggc ggacgcaacc caagcgcgct cttcgcgcgt gacccaggtt ccggatctcg
     1441 tgttgctgga catctggatg ccggataccg acggcatcac gttgttgcgc gagtggtcgg
     1501 tcaccgacgg ctacgactgt ccggtcgtca tgatgtcggg tcacggcacg gtcgagaccg
     1561 cggtcgaggc gacgcgcctg ggcgccttcg atttcgtgga aaagccgctg tcgctgacca
     1621 aactgctgcg caccgtcgag cgcgccctcg atgccggacg ccgcaagcgg ctgtccgcgc
     1681 gtacccacgg accgacgctc gcggtgccgg tcggcaagag caaggtgact caagcgctgc
     1741 gcgagcaagt gcagcaggcc gcgacgagtt cctcgccggt gctgctgctc ggtgaattgg
     1801 gatccggacg ggaagcttat gcgcgctttc tgcactcgct gagcgcacgg gcgacgaagc
     1861 cgtttttcat ggtggttgcc gcaagcctcg gcagcgaccc gcacagccag ctgttcggca
     1921 gtgagcggga tggccgcgtg gagctcggtg cactcgatca agccgccggc ggctcgttgt
     1981 acttgaatgg gctcgaggac ttgaacagcg aagcacagcg cgcgctggcg ggggccattg
     2041 aacagaacgg ctacacccgc gtgggcgggc ggcagcgcgt gcccctgaat gtccgctgga
     2101 tcagttcggc gcaggacacg tttgaattgc gtgcctcgcc ggagccgttc cgccgcgacc
     2161 tcgtatcgca cttgaatgtg atcacgctgc gggtgccgcc gctgcgcgat tacgccgagg
     2221 acgtgcccga tttattgaga tattacgtgg atcggctggt cgacgatcaa cacctgccgt
     2281 tgcgccgctt cagcgtggcc gcgcagaacc ggctgcgaaa ttatccctgg cccggcaatg
     2341 tcggcgaact caagagtctg gtgcagagag tgctcgtgct cggcgtcggc gaggagatac
     2401 ggctcgagga aatcgagcgc gagttggcgg tgaagctcgc cgtggatgag ccgctggtga
     2461 agcaggactt gctggcgctg ccgctacgcg aggcgcgcga gcagttcgaa cgggcgtatc
     2521 tgcagcaaca attgctgctt tgcaacggca aggttggcca gttggccaaa cgagtgggaa
     2581 tggagcgtac gcatctatac cgtaaattgc gggcattagg cgtcgatttt cgccaattag
     2641 gagacgattg agcgcgagcg tagagttcag tcgccgactc ggatgaccag tacatcgacg
     2701 tcggctttgc gcaggatctg ccgtaggcgg ttgagttcat ccagcttgga aatcggccca
     2761 atgcggatgc ggtgccaggt atcgttgtcg acggacactt tctgcacctt cgattccacg
     2821 ccttgcaggg ccagttgcgc gcgcactcgg tcagcgtcgg cgaaattctt gtacgagccg
     2881 gcttgcagta cgtacgtgcc gcggcgggtt tcgggaacgg atttggtgtc cggccggacg
     2941 tccttgtcct tctccgggac cacgacctcg aatttcggca gcatgtcgta gaagtcgtag
     3001 gatttcgccg gctcctccgc gctcgagtcc gccgtttcgc catcggggaa ttcgctgccg
     3061 cggaatttct ttttatccac cttgccggcc cgggcgatcg gcgcatccgg tcgatggtcc
     3121 ttgatataca cgatgccggc gacgcttagg cccgcagcca atccccacac cacgcccgtc
     3181 cacccggaga agccggtggc cttgccgcgt ctctgcttgt agtcgcgcgc catggaatct
     3241 tgagccgtta catcgtgtcg ggcgcagaaa cacccagcaa ttcgaggccg ttgcgaatga
     3301 cggtttgcgt cgccaacgca agatcgatcc gcgcgttgcg cagtgccgcg tcctccacga
     3361 tgaactggtg cgcgctgtaa tcggtgtgaa aatcgttcgc caggtcccgc aggtagtaca
     3421 ccaacgtgtg cggcgctcgc agctgcgcgc attgccggac gatctcaggg tatgcactca
     3481 cgcgcttgat gagctgcagc tcctggggtt cgacgagcgc actcagattg gccaagccgt
     3541 gcgccgggtc gtgggtcagt ccgcgctcct gcatctgccg catcacgctc gccacccgcg
     3601 cgtgcgcgta ctggatgtag tagacagggt tgtcgttgct gcggctggtg gcgagttcca
     3661 agtcgaagtc caggtgctga tcgttgctgc gcatgacata gaacagccgg gccgcatcgt
     3721 tgcccacttc gttgcgcagt gcgcgcagcg tcacgaactc gcccgagcgc gtcgacatct
     3781 gcgccttctc gccgccgcga aacaacgata cgaactgcac gagccgcact tcgaaggatt
     3841 ctgcagggcc gccgagcgcg gtcaggccgg cacgcagccg ggtgatgtag ccgtgatggt
     3901 ccgcgcccca aatgtagagc aaatgatcga acccgcgttc gagcttgttg aagacatagg
     3961 caatgtccga tgcaaaatac gtcttgacgc cgttgtcgcg caccatcacg cgatccttct
     4021 catcaccgta gtcggtgctc ttgaaccaca aggcaccgtc tttgacgtag gcatgtccgg
     4081 ctcgcttcaa ttcggcaatt gctcgatcga tcgagccgtc gacgctcaag gagcgctcgg
     4141 agaaccagca gtcgaagctg acgccgaatt cctgcaagtc ctcgcgaatg tcgccaagga
     4201 tgcccgccag cgccaaatcc agcacgcgcc gccaggcggc atctccgatc aaggagcggg
     4261 cgcgcgatat cagcgcatcg atatagacat ccttgtcgcc tccctgcggc gcgtccggcg
     4321 gcaagtcgcg gaacacctgc gcagcgctgc gccgcagttg ctcgccccgc gcggcataca
     4381 gctcagcgcc gatcgcggtg atgtagtcgc cgcgatagcc gttggcagga aacgggaatt
     4441 gctcgccgca gcgttcgagg tagcgcagcc aggtgctggc cgccaaaatc tccatttgac
     4501 ggccggcgtc gttgatgtag tactcgcgct gcacgcggta tcccacggcc tccagcaagt
     4561 tggcaacgct cgcgccatac gctgcatgtc gtccgtgacc gacgtgcagc ggcccggtcg
     4621 ggttcgccga cacgaattcc acctgcaccg accggccggc gcccgcactc gagcggccgt
     4681 agcgccgagc ttcctcgagc accttggcga tttcgccgtg atacgcatcc ttcgcaagaa
     4741 agaaattgat gaagccggca cctgcgatgt caaccttggc aagcgccggg cagggcggca
     4801 gagagcgcag cagcaattgc gccaagttgc gcggattctg ccgtgtcgcc ttggcgagcc
     4861 gcatcgcgag attgctggcg aaatcgccat gctgcgcatc gcgggtagtt tcgacttcca
     4921 cagccacgcc gcgggcatcc atcggcacga catcttccgg cacggccgcc agcgcttgtc
     4981 gcagtagatt ttcaatcaga gtcttcaagg cgagtcccgt gcagagaaaa gggtcggcag
     5041 tttaccgctg caggcgcgcg cacacatctc aatcgacttc gagcggatcg acgtcgatcg
     5101 accagcgcag gccgggtggg ccggccaggg actcaacccg cggcagccag tcgccgagaa
     5161 aacgctgcaa agtggcgcgc gcggaggtct caatcaagag atgggcgcgg aagtgatcgg
     5221 ctctgcgggc gatcaaggcg ctggcaggac ccaagacttt gacggcggtt tcctgcgacg
     5281 cgcttgcgca ctgggcggcg gcgcgcagga aagcgtccag tccttgaaga tcttttgcct
     5341 cggcgcgcag cattgcgagc ctggaatacg gcggccaacc tgcgtcgcgc cgctcttgca
     5401 gagcgctttg ggcgaatccc tcatagccat gcagcatcag ccgttgcagc agcggatgct
     5461 cggggaactc cgtttgaatc aatacctcgc ccgcgagggt tccgcggccg gcgcggccgg
     5521 cgacctgcgt gatggtctgc gcgagccgtt cggtggcgcg aaagtcgctg gcgaacaaac
     5581 cctggtcggc atccaagatc accaccaaac tcacttcggg aaagtgatgg cccttggtca
     5641 gcatttgcgt gccgaccagg atccgcgccg cgccgctgtg gacgcggtcg agcacgtcct
     5701 gcacggcgcc gcgcgcggcg gccgtgtcgc gatccagccg cgcgagcggc gcctcgggaa
     5761 acagccgcga cagggtctct tcgacccgct cggtgccctg tccaatcgga tgcagcggct
     5821 ggccgcaact tccgcaaatc acgggcaccg cctcgtgagc gccgcagtga tggcaacgca
     5881 gttgctgcgc gcggcggtgc agagtcatcc gcgcatcgca atgcgcgcag ctcgcgaccc
     5941 agccgcagct gttgcagaac aagctcggcg cgtagccgcg gcgattcaag aacacgatga
     6001 cttggccgcc ggctttgaga tgttgcgcca tggcgagcat cgccggcgtc gacagccctt
     6061 ggtcgctggc gcacttgcgc agatcgagca gcgacattcg cggcgcctgc actgcgcccg
     6121 gccgctgcgg cagcaggtgc ttcatgtagc gcccggaggc ggcgttctcg agagtttcga
     6181 gcgacggtgt cgcggaaccc aaaatgacgg gcacgcccgc gcgccgcgct cggacgaccg
     6241 cgagatcgcg cgccgaatag cgaaaaccct catgctgctt gtacgacatg tcgtgctctt
     6301 cgtcgaccac gatcaatgcg agcttcggca acgaggtgaa cacggcggaa cgggtgccga
     6361 tgacgatgcg cgcctcgccg ttgtgcgcag accgccaggc gtcgcgccgt tcgccgcccg
     6421 tgaggcccga atgcagcgcc acgacgccgg aactgaagcg gctgcggaag cgatcgacga
     6481 gttgcggcgt caatgcgatc tcgggcacga ggaccagcgc ttgggcgccg tcggcgattg
     6541 cggcggcgat ggcgcgcaaa tagacctccg tcttgccgct gccggtcacc ccgtaaagca
     6601 gatgcactgc aaacccgggc aaggtcgcaa gtacggctgc gacgcagtcg gcttggtgct
     6661 ggttgagttc gaccgggctc ggcagcgaca cagttgcgcc gtgcgttggc gcagcgtgcg
     6721 gcacggcgcg ctgcgccgcg gtccaaccgc gggccgccag ggcgcgcaag agcctaagct
     6781 cgaacgtcgc gcgaatgtcc tcggtggtgg cgctatgccg gtgcccgagc cacgcgagca
     6841 gcgcgcgctg ttgcggagcg cgcctcgcgg tgggcgccga cagttcctcg cgcccggcct
     6901 cggtcaggga ccagcactcg gccggcggca gcgccggctg gccggctctg aggctcgccg
     6961 gcaaggcggc cgcgaaaacc tcgcccagcg gatggtggta atactccgca gcccagcaca
     7021 gcaagtcgaa ggtcaccgga tccacgatcg gacgctcatc caaaatctcg acggccgttt
     7081 tcagtttggg caacggcacc gcggagtcgg ccgcgacccc caccaggatg ccgatcaatt
     7141 gccggcgccc gaagggcacg cgaacccgca ggcccgggcg aagccgctgc gcggcacccg
     7201 ggccggtcag cgatgccggc ggccgataat caaagacccg acgcagcggc gtgtcgagcg
     7261 cgatctgcag aatcaaggca ttccttttgt ccacaaaaac tgtggataag tctgttgaaa
     7321 agcggggatc atcgacctta agtgcccgtt ccgtttcggg tcctatccaa attggcgaaa
     7381 aagtgtccga aatcgaaatg aatcatctaa attcaatggg ttacaacgcc ctctagatgc
     7441 cgcaatgagg tgttgctgca attattgcaa tggatgtcac agtcggtgtg tgtaagtcgc
     7501 tgatcttgcg caagccgtgc cgtcaaccgc cgcgcccgat acgcgtttat acctggaacg
     7561 acgacgctcc gtcgacgtta cgaggacgag gcatgccccg ctttgatacg ctgaactcag
     7621 cttggatggt ctgggataat cccgctccag caccggagga tgcggttctg gagtacacgc
     7681 gggaactcga gaaattcttg gctgaggtcg agcgccgggc gttccgcatg gcgcaggtgg
     7741 cgttgcgcga tcctgacgac gcgatggatg tggttcaaga cacgatgctc aagctggcgc
     7801 gcaactacgc ctcgcgcccg agcgccgaat ggcggccgct gttctaccgg atcctggaaa
     7861 acggcatacg cgatctgcaa cggcgcagat ccgtgcgcaa tcgattcatg acgtggctgc
     7921 cgggcgcgaa acaggatccc gacaacgaag cgcaggatcc gctggagacg gtcgcggaca
     7981 catctgcgcc cattccggag gagctgatgc gcgatcaagc gatgcagcag ctcgaggtgt
     8041 cgttgcgtgg cctgccggcg cggcaacgcg aggcgttcat gctgcgcaat ttcgaaggat
     8101 tggatgtggc cgaaacggcg accgccatgg gttgttccga aggcagcgtc aagactcatt
     8161 actcgcgcgc agtgcatacg ctgcgcgaac aactgggcga ggtctggtga tgaacgaaca
     8221 attgcctgtg caacgccgtg atgcgctcga ggagcgcagc cgcaggctgt tcctcgagag
     8281 tgtcgagggc atggactttg cgacgcgctc gcgcctccgg caggcgcgaa ctgcggcgct
     8341 cgcggcagca tcgacccgcc ggccgtggtt ttcgcgcgcc ggtgttctgg cgccggccgg
     8401 ggtcaccgcc gccgcggttc tcggcgcctt cctttggctg ggatcgccat tgggaccgca
     8461 cgccgttaca gtagtcgatg gccagtcgaa tttcgaggat ttggaacttg tcgcagcgtc
     8521 ggacgaggga tcgggcgacg cgctggacat gttgcaagac gatattgaat tttatgattg
     8581 ggccgataaa gcggcggcga attcggggcc ggaagcccag agttgaactc cggttgagct
     8641 ggggtaggca ttggctgttg tgggtcacca tcgggagcgc g
//
LOCUS       DASWZT010000077         1911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_19926, whole genome shotgun sequence.
ACCESSION   DASWZT010000077 DASWZT010000000
VERSION     DASWZT010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19926"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="VGE92_02050"
     CDS             complement(<1..944)
                     /locus_tag="VGE92_02050"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEY1312625.1"
                     /translation="MIREISALDSAAIRRDVLPDKQPAIFRGLVREWPAVKHALESAA
                     ALVRYLARFDSGKSVDALLAPPEVDGEIFYNATMTGFNFVRNRLPLGAVAQQVLRYSQ
                     FPKSPAVAAQSALIRDCLPGFRDENKLALVEDDVLPRIWLGNRITTPTHVDEWNNVGC
                     VVAGRRRFTLFPPGQISNLYIGPLDFAPTGAPMSLVSLRNPDFERFPKFRDALRASQT
                     AELAPGDAIFIPPLWWHHVESLEPFNLLVNYWWHDLAGDGALADSAFDALLHGILSIR
                     ALPPATRRAWGDFFEHYVFGDQVGSLGHISAERRGILGT"
     gene            complement(1057..>1911)
                     /locus_tag="VGE92_02055"
     CDS             complement(1057..>1911)
                     /locus_tag="VGE92_02055"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEY1312626.1"
                     /translation="AEHFPFDVWHGYHFDSVLLGQFLHKKSLERGVRYLAAHVTHANL
                     DERGAIASLGTREGPTIDADIFVDCTGFAGLLITQALQTPFVSFAENLFNDAAIAMPT
                     PIGDSLPSETGATALKNGWAWKIPLTQRFGNGYVYSTQFCTPDAAERELREHLGLLDS
                     DTPARHLKMKIGRVTKHWNKNCLAVGLSQGFIEPLEATALLFIQQTAAAFVDFLEQGD
                     LSEAAHDRFNAQVNQHFEGTRDHIVTHYKTSSRKAIPCFPGTPSWRAWVFSRIRRRCA
                     QPAQMHTA"
BASE COUNT          368 a          562 c          645 g          336 t
ORIGIN      
        1 gtgccgagaa taccgcgccg ctcggcggaa atatgcccga gcgacccgac ctggtcgcca
       61 aacacgtagt gctcgaaaaa atcgccccag gcgcgccggg ttgcgggcgg cagggcgcgg
      121 atgctgagta tgccgtggag cagcgcgtcg aacgccgaat ccgcgagcgc gccgtcgccg
      181 gctagatcat gccaccagta gttgacgagc aggttgaagg gctcgagcga ctcgacgtgg
      241 tgccaccata gcggcggaat gaaaatcgca tcgccgggcg cgagctcggc cgtttgggaa
      301 gctctgagcg cgtcgcgaaa cttcgggaag cgctcgaagt cgggattacg cagcgatacc
      361 aggctcatcg gcgctccggt cggcgcgaag tcgagcggac cgatgtacag attggaaatc
      421 tgccccgggg gaaacaacgt gaagcgccgc cgcccggcca cgacgcagcc gacgttgttc
      481 cattcatcga catgcgtggg cgtggtgatt cggttgccga gccagatgcg cggcaggacg
      541 tcatcctcga cgagggccag tttattctcg tcgcggaacc ccggcagaca atcgcgaatg
      601 agagcgctct gcgccgccac cgccggcgac ttcggaaatt gggagtagcg caacacctgc
      661 tgcgcgaccg cgccgagggg cagccggttg cgcacgaagt tgaagccggt catcgtcgcg
      721 ttgtagaaga tttcgccgtc gacctcgggg ggcgcgagca acgcatcgac ggatttgccg
      781 ctgtcgaagc gcgcgagata gcggaccagc gcggcggcag attccagcgc atgcttgaca
      841 gccggccact cccggaccaa gccgcggaat atcgccggct gcttgtccgg cagaacatcg
      901 cggcggatcg ctgcgctgtc gagcgccgag atttcgcgaa tcaccaaaag tacacctgca
      961 gagagtcgcc actgcggcgc cggggaatgt tgcccaggca ggcgcgatgg tcgggaaagt
     1021 tctgtgcgct gcgatcgagc agatcgtcga tgtcggtcaa gcggtatgca tctgcgcggg
     1081 ctgcgcgcag cgtcgccgga tccgggaaaa tacccatgcc cgccaggatg gcgtaccagg
     1141 aaaacacggg atagcctttc ggctgctggt cttgtaatga gtgacgatgt ggtcgcgggt
     1201 gccctcgaag tgttggttga cctgcgcgtt gaagcggtcg tgcgccgcct cgctcaagtc
     1261 tccttgttcc aagaagtcga cgaaggccgc ggcggtttgc tggatgaaga gcagcgcggt
     1321 cgcttcgagc ggttcgatga agccttgcga cagtcccacg gcaagacaat tcttgttcca
     1381 gtgcttggtg acgcgtccga tcttcatctt caggtgccgc gccggcgtat ccgagtcgag
     1441 caatccaagg tgttcgcgca actcccgttc tgccgcatca ggcgtgcaaa attgcgtgct
     1501 gtagacgtag ccgttgccaa agcgctgcgt cagcggaatt ttccaggccc agccattttt
     1561 cagggcggtg gcgccggttt ccgagggcag cgaatcgcca atcggcgtcg gcatggcgat
     1621 cgccgcgtcg ttgaatagat tctccgcgaa gctgacgaat ggggtctgca acgcctgcgt
     1681 gatgagcaat cccgcgaaac ccgtgcagtc gacgaagatg tcggcgtcga ttgtgggtcc
     1741 ttcgcgcgtg ccgagcgagg cgatggcgcc gcgctcatcg aggttcgcat gggtgacatg
     1801 tgcggccagg tagcgcacgc cgcgctcgag cgatttcttg tgcagaaact gacccaacaa
     1861 cacggaatca aagtgatagc cgtgccaaac gtcgaacggg aagtgttcgg c
//
LOCUS       DASWZT010000078         8181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_29619, whole genome shotgun sequence.
ACCESSION   DASWZT010000078 DASWZT010000000
VERSION     DASWZT010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8181
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29619"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VGE92_02060"
     CDS             complement(<1..316)
                     /locus_tag="VGE92_02060"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1312627.1"
                     /translation="MKRIAESARSVDVILETDVLVVGSGPGGLAAALAAARAGANTAL
                     IDRNGCFGGNITQVGVEGFAWYRHEHTVDCEGIGIEFETRAKQMGAAMPEPQSLSHAL
                     DAE"
     gene            652..3330
                     /locus_tag="VGE92_02065"
     CDS             652..3330
                     /locus_tag="VGE92_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1312628.1"
                     /translation="MNNNQKLAYAISAVLSGYTGAYAASDADTAANSGIDEIVVTAQR
                     RSESIQNVPITIQAITGDQLAQLNVTTFDDVLKYLPNVTFGSNGPGQGNIYMRGLSAG
                     FAGNQSSAAVAPFPNVATYLDDQSMTFPARNADVYMADMERIEVLEGPQGTLFGGGAE
                     AGAVRYITNKPKLNVTEGNAEGSYGTTAHGDPNSAITAMINVPLIEDVLAVRAVIYND
                     RRGGYIDNVPSTFTRLPTDNGPATYHLAYPANAASANNFALAGRAQNPVTYQGIRVSA
                     LWQIATDWSLLVQQSYQNMDAEGLDEQFPIGSEGQKLGPWEVNEFSPSYDKDKYENTS
                     WTVNGKIGDLKLVYTGAYLDRTIDNAMDYTNYARSQGGFYYTCAGGGAPIGKGVGPLT
                     CYSPISSWHDYVETTHQSHEIRLSTPDDWRLRGLVGVFWEDLDIKDDINFLYKSIPSC
                     TPANLLLYQAGTQVCVGNVIPAPGYAAIDPTERNDSTAFGEDLERGYKQAAAFTSIDF
                     DLIPKVLTLTGGTRYYHYSETFQGSQYSTSTGCAGVPNGQCVGGKLLPSDHDATYSGF
                     RSRGNLTWHVTPDAMVYYTYSQGFRPGAGNRKDSTEISIAVTETSPGVYVPAVGPVAS
                     PTLVKQYNKPYTYPPDTLINNEVGWKTEWFDHRLQVNGSGYVMNWNNVQTLIYNPPIS
                     GNTTFGVQGPDYRIKGVELQIVGRVTDGLTVQGSVSDNSASQTNSPCLKSSNAVAANP
                     TPIGSCITQVYSSTLSQNVPLLNPLGAVGSTPAFSPKIEYNLRARYDWHVNDYKMFFS
                     AGAQHVGDMQNEPSSFASGAGVLNPTTTWLRYDQPAYTTYDAAFGIAKDNWHVEAFGT
                     NLGNSDASLFTTSGQFIEAQVPLRPRVLGLKVGMKF"
     gene            3400..5439
                     /locus_tag="VGE92_02070"
     CDS             3400..5439
                     /locus_tag="VGE92_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY1312629.1"
                     /translation="MNTATAPLTSTVESEVARIRALIKERRFVEGVTAATLLRQRVPE
                     NRDVLYLLALGQRQLTRTADALATIGELERCHPGYSRLYQERGHCYVAMQDAPQAIQA
                     FLRAVAINPALPASWSMLEGLYRMAGDTQNAALARAHVAKLKTLPREVISATGLFSDG
                     DVLAAERMIRAYLLAHGDHIEAMRLLARIGAAHDVLDDAELLLQAVLERVPDYVAARF
                     DYASVLLGRHKHRQALTELDKLLAIDPGNRQYLTSYATGVVGLGEHARAIALYEDLLR
                     SASPGSPEGAELHLSIAHSLKTLGRQAEAIAAYRAAAQDRADFGDAYWSLANLKTYRF
                     ADAELGRMRAAEAAPATAPVDRYHLCFALGKAYEDRGDYAESWSYYQRGNALKRAESR
                     YRPELIERNTAQQKKVCTREFFERRTDYGVASTAPIFVVGLPRSGSTLIEQILASHSA
                     VEGTHELADIPRCVLELQGREPDFEAPRYPGVLAQLAPQEYVRFAEKYLRDTLVYRTD
                     KPHFIDKMPNNFRHLGLIRLLFPNAKIIDARREPMACCFGNLKQLFARGQEFTYSIRD
                     IARYYRTYLELMEHFEALLPGGVLRVLHEDLVDDLEGNVRRILDFCELPFEPACLEFH
                     RTERSIVTASSEQVRQPIFREGLEQWRHYEPWLSPLREALGDALERYRPDATRTQ"
     gene            5396..7564
                     /locus_tag="VGE92_02075"
     CDS             5396..7564
                     /locus_tag="VGE92_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY1312630.1"
                     /translation="MHSNATVQMPPGRNEPCRCGSGRKFKHCCGKTPAAVAAVRRPPS
                     DAERAWALGRALEDQGLLERAADSFARATALYPEFAEAHNDLGQVLLRLGRTADAVAS
                     CQRALALKPEFAAAIGNLANAKRAQGSIAAAIEGYRRVIALEPRLAEGHRNLGSALFE
                     AGETDAAIACLRTAIELRPEFVGAVTQIARALGAVGRAAEAIPYYERLLAKGSSAELL
                     NEYATLLVGLRRYEQSVRSYRDALARAPTDAKLHANLGHALHCLGDFRAAIEHCRRAI
                     ELEPQLAEAHLNLGNSLLALNLLYEADAAYRSGLKVAPDHAALHTAHAMAERALGQLA
                     EAQASARRALALRPGAADTLALLGSLAIDHGRFDEAEELLRKALLIDPELPEALTSLM
                     AVRRMGPAEAPWRDAAERVLARGLSVAHAINLHHALGKYYDDVDDRDAAFEHHRLGNE
                     LARRSRIRYDRAEWTQRVARTIAAFDRNALAALSSCGVASERPVFVVGMPRSGTSLTE
                     QILASHPEVYGAGELLYWIFAADAERAATLPQRAAKIIELGRSYLEGLALQAPDASRV
                     VDKLPVNFANIGLIHAALPGARFIHLERNALDTCLSIYFQGFSAAHPYATDFGDLAHY
                     YHEYRRLMAHWRAVLPPQSLFEVRYEDLVDDPQGWSRRMLEHIGLPWDSRCLEFYRTD
                     RPVLTASNWQVRQPIGKGSLDRWRRYEGFIGPLRAALGEG"
     gene            7628..7885
                     /locus_tag="VGE92_02080"
     CDS             7628..7885
                     /locus_tag="VGE92_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312631.1"
                     /translation="MLRLLRLWRIAGKDLSLLWFALRQRHRPIWLLPAVVLLGLYALE
                     PLNFAIPLLGVVDDVILLPLALHFMARCLPAEIRYGFARRG"
     gene            complement(7905..>8181)
                     /locus_tag="VGE92_02085"
     CDS             complement(7905..>8181)
                     /locus_tag="VGE92_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312632.1"
                     /translation="EKLSSVHKDQVFAQKDHDDHDHSEHPQPGDDHEAASLGSYLLKR
                     YFLLVSLALTASTLLGLWMGLMQVRRKRAGWWLLGVGIVVPVALCFF"
BASE COUNT         1492 a         2634 c         2594 g         1461 t
ORIGIN      
        1 tttcggcatc gagcgcatgg ctcaaggact ggggctcggg catagccgcc cccatctgct
       61 tggctcgagt ctcgaactcg atgccgatgc cctcgcagtc gacggtgtgc tcgtgccgat
      121 accaggcaaa gccctcgacc ccgacttgag tgatatttcc gccaaaacag ccgttgcggt
      181 cgatcaacgc cgtgtttgcg ccggcgcgcg cggcggcgag tgccgcggcg agaccgccgg
      241 gtccgctgcc gacgaccagc acgtcggtct ccagaatcac gtccaccgag cgggcggatt
      301 cagcaattct cttcataagg gctacatcct taggatcgcc ccgggggggc aatcggctgc
      361 ccattcgtaa cacaggattg tgcaaaaggg cacgtctggc ccccgggaaa tccaccgacg
      421 cgcgcggcga cttgaatgct gcgtatcatt taagtcatag tactgtcata aatacgtaat
      481 ctgctgtgcg tacaaactag cgcagcgggg aattggcgag ccgaagtatg gatggatgga
      541 ggctcgatcg atatttggga tgtcactgcc gttcggcgcc tttcgagcgc gacgatatcg
      601 ttcgtcgcgc gccctcgttt cgtatcgctt tgaattttca gggagacatg cttgaacaac
      661 aatcagaagc ttgcctacgc aatctctgcc gtccttagcg ggtatacagg cgcctatgcg
      721 gcgagcgacg ccgacacggc cgccaactcc ggaatcgatg aaatcgtcgt caccgcgcag
      781 cgtcgttcgg agtcgattca gaacgtcccg atcacgattc aggcaatcac tggcgatcaa
      841 ctcgctcagt tgaacgtgac gaccttcgac gacgtcctca aatacctgcc gaacgtgacg
      901 ttcggctcga acggcccggg ccagggcaat atctacatgc gcggcctctc cgcgggcttc
      961 gcaggcaatc agtcgagcgc ggcggtcgcg cccttcccga acgtcgcaac ctacctggat
     1021 gaccagtcga tgacgttccc ggcgcgcaac gccgacgtgt acatggctga catggagcgc
     1081 atcgaggtgc tcgagggtcc ccagggcacc ctgttcggcg gcggcgccga agcgggcgcg
     1141 gttcgttaca tcacgaataa gccgaagctc aacgtcacgg aaggcaacgc cgagggctct
     1201 tacggtacca ccgcgcacgg cgatcccaac tccgcgatta cggccatgat caatgtgccg
     1261 ttgatcgagg acgtcttggc agtccgtgca gtcatctaca acgatcgtcg cggcggctat
     1321 atcgacaatg tgccgagcac gttcacgcgc ctgccgacgg ataatggacc cgccacgtat
     1381 caccttgcct atccggcgaa cgcggccagc gcaaacaact tcgccctcgc ggggagggcg
     1441 caaaacccgg tgacctatca gggcatccgc gtctcggccc tctggcagat cgccaccgat
     1501 tggagcttgt tggtccagca gagttaccag aacatggacg cggaaggctt ggacgaacaa
     1561 ttcccgatcg gatcggaagg ccagaagctt gggccctggg aggtgaacga attctcgccg
     1621 tcctatgaca aggacaaata cgagaacacg tcttggaccg tcaacggcaa gatcggcgac
     1681 ctgaagctgg tctataccgg cgcctacctc gaccgcacga tcgacaacgc catggactac
     1741 acgaactacg cccgttcgca gggcgggttc tactacacct gcgccggcgg cggcgcaccg
     1801 atcggcaagg gagtgggacc gctcacctgt tactcgccga tctcctcgtg gcatgactac
     1861 gtcgagacca cccatcagag ccacgaaatc aggctgagca cgccggatga ctggcggctg
     1921 cgcggcctgg tcggtgtctt ctgggaggac ctcgacatca aggacgacat aaacttcctg
     1981 tacaagtcga tcccctcatg cacgccggcg aacttgctgc tctaccaggc tggtacccaa
     2041 gtgtgcgtcg gcaacgtgat tccggctccg gggtacgctg cgatcgatcc gaccgagcgc
     2101 aacgacagca ccgcgttcgg cgaagacttg gaacgcggtt acaagcaggc ggcggcattc
     2161 acctcgatcg atttcgactt gatcccgaag gtcctgactc tcacgggtgg cacgcgctac
     2221 taccactaca gcgagacctt tcagggttcg cagtactcga cgagcaccgg atgcgcgggc
     2281 gtgccgaatg ggcaatgcgt cggcgggaag ctcttgccat cggaccacga tgcaacctat
     2341 agcggctttc gcagccgcgg caacctgacc tggcacgtca cgccggacgc gatggtgtac
     2401 tacacctact cgcagggctt tcggccgggc gccggcaatc gaaaggacag caccgagatc
     2461 tccatcgccg taaccgaaac gtccccgggc gtgtacgtgc cggccgtcgg tcccgtcgcg
     2521 agcccgacgc tggtcaagca gtacaataag ccctacacgt atccacccga cacgctgatc
     2581 aacaacgagg tcggctggaa gacggagtgg ttcgaccacc gcctgcaggt gaacggctcc
     2641 ggatacgtca tgaactggaa caacgtccag acgctgatct acaacccgcc gatctccggc
     2701 aacacgacgt tcggcgtaca ggggcccgac tatcgaatca agggcgtcga gttgcagatc
     2761 gtcgggcggg tcaccgacgg cctgaccgtg cagggttcgg tgtccgacaa cagcgcttcg
     2821 cagaccaact cgccgtgtct gaaatcgtcg aacgcggtgg cggctaaccc gacgccgatc
     2881 ggcagctgca tcacgcaggt ttattccagt acgctgagcc agaacgtgcc gctgctgaac
     2941 ccgctgggcg ccgtcggttc gacacccgcc ttctcgccga agatcgaata caacctgcgc
     3001 gcacgttacg actggcacgt gaatgactac aagatgttct tttcggccgg cgcgcagcac
     3061 gtgggcgaca tgcagaacga gccgagcagc ttcgcatccg gagccggcgt cctcaacccg
     3121 acgacgactt ggctgcgcta cgatcagccg gcgtacacga cgtacgatgc ggccttcggg
     3181 atcgcgaagg acaattggca cgtcgaggcc ttcggcacca acctcggcaa ctcggatgca
     3241 agcctcttca ccacgtcggg gcagttcatc gaggcgcagg tgccgctgcg gccgcgcgtg
     3301 ctcggcctca aggtgggcat gaagttctaa gggcgttgcc cgagggctta gactctgaag
     3361 gcgggcagcg atgcccgcct tcttgttatt ctgatgccca tgaacaccgc aaccgcgcct
     3421 ttgacctcga ccgtggagtc cgaggtcgcc cgcatccgcg cgctcatcaa ggagcggcgc
     3481 ttcgtcgaag gtgtgacggc cgcgaccttg ctgcggcagc gggttcccga gaaccgcgac
     3541 gtgctttatt tgctcgcgct cggccagcgc cagctgacgc ggaccgcgga tgcgctcgca
     3601 acgatcggcg aactcgagcg gtgccacccc ggctacagcc gtctgtatca agagcgcggt
     3661 cattgttatg tagcgatgca ggacgcgccg caggctatcc aggcattcct gcgggcggtc
     3721 gcgatcaacc cggcgctgcc ggcgagctgg agcatgctgg aaggcctgta tcgaatggcg
     3781 ggcgataccc aaaacgcggc actggcgcgc gcccatgtcg cgaaactcaa gacattgccg
     3841 cgggaagtca tctccgcgac cgggctgttc agcgacggcg atgtcttggc ggcggagaga
     3901 atgatccgcg cctacttgct cgcacatggc gatcacatcg aggcgatgcg tttgcttgcg
     3961 cggatcggcg ccgcccacga cgtgctcgac gacgcggagt tgctgctgca agccgtgctc
     4021 gagcgggttc ccgactatgt tgctgcccgc ttcgattacg catcggtgct gctgggccgg
     4081 cacaagcacc ggcaggccct gaccgagctc gacaaactgc tggcgatcga tcccggtaat
     4141 cgtcagtacc taacctcgta tgcgacgggc gtcgtcggcc tcggcgagca cgcgcgcgcg
     4201 atcgcgctct atgaagatct gttgcgcagc gcatcgcctg gctcgcccga aggcgccgaa
     4261 ctgcacttgt cgatcgcgca ttccttgaag acgctcggcc gccaagccga ggcgatcgct
     4321 gcgtaccggg ccgcggcgca ggatcgtgcg gattttggcg acgcgtactg gagccttgcg
     4381 aacctcaaga cctaccgctt cgccgacgca gagctcggcc gcatgcgcgc cgcggaggcc
     4441 gcgccggcga ccgcgccggt cgatcgctat cacctgtgtt tcgcgctcgg caaggcctac
     4501 gaggatcgcg gcgactatgc cgaatcgtgg agctattacc agcgcggcaa cgcattgaag
     4561 cgcgccgaga gccgctaccg cccggaactc atcgagcgca ataccgcgca acagaaaaag
     4621 gtttgcacgc gagaattctt cgaacggcga acagactacg gggtggcgag cacggcgccg
     4681 atcttcgtgg tcgggctgcc ccgttccggc tcaacactga tcgaacagat tctggcctcg
     4741 cactccgcgg tcgaaggcac acacgaactc gccgacatcc cgcgctgcgt gctcgaactg
     4801 cagggccgtg aacccgattt cgaggcgccg cgctatcccg gcgtactcgc gcagctcgcg
     4861 ccgcaggaat acgtgcgctt cgcggagaag tatctgcgcg acacccttgt ctatcgcacg
     4921 gacaagccgc acttcatcga caagatgccg aacaacttcc ggcacctggg cttgatccgc
     4981 ctgctgttcc cgaatgcgaa gatcatcgac gcgcgccgcg agcctatggc ctgctgcttt
     5041 ggcaacctca agcaactgtt tgcgcgcgga caggagttca cctacagcat ccgggacatc
     5101 gcacgctact accgcactta cctcgagctg atggagcatt tcgaggcgct gctgccgggc
     5161 ggcgtgcttc gcgtgctgca tgaggatttg gtcgacgacc tcgagggcaa tgtccggcgg
     5221 atactcgact tctgcgagct gcccttcgag ccggcctgcc tggagttcca ccgcaccgaa
     5281 cgcagcatcg tcacggcgag ctcggagcaa gtgcgccagc ccatctttcg cgagggactc
     5341 gagcagtggc gccactacga gccatggctc agcccactgc gcgaggcgct cggcgatgca
     5401 ctcgaacgct accgtccaga tgccacccgg acgcaatgag ccctgccgtt gcggcagcgg
     5461 gcgtaagttc aagcactgtt gcggcaagac gccggctgct gtggccgccg tgcgacggcc
     5521 gccgtcggac gcggagcgcg cgtgggccct tggacgggcg ctggaggatc agggtttgct
     5581 cgagcgggcg gcggatagct tcgctcgcgc gaccgctctg tacccggagt tcgccgaggc
     5641 gcacaacgat ttaggccaag tactgctgcg gctgggacgc acggcggacg cggttgcgag
     5701 ctgccaacgc gcgctcgcgc tcaagcctga gttcgccgcg gccattggca acctcgcgaa
     5761 tgccaagcgc gcgcagggct cgatcgcggc cgcgatcgaa ggataccggc gcgtgattgc
     5821 gctcgagccg cgactcgcgg agggtcatcg caacctcggc agcgcgctat tcgaggctgg
     5881 cgagacggac gcggcgatcg cctgcttgcg gacggcgatc gagctacgcc cggagtttgt
     5941 gggcgcggtc acgcagatcg cgcgcgcact cggcgcggtc ggtcgcgccg cagaagcgat
     6001 tccctactat gagcggctgc tggcgaaggg ctcgagcgca gagctcttga acgagtacgc
     6061 gaccttgctc gttgggcttc gccgctacga gcaatccgtg cgcagctacc gcgacgcact
     6121 cgcgcgtgcg ccgaccgacg ccaagctgca cgccaactta ggtcacgcgc tgcactgtct
     6181 gggcgatttt cgcgcggcga tcgagcattg ccggcgcgcc atcgagctcg agccgcagtt
     6241 ggcggaggcg cacctgaacc ttggcaattc gctgcttgcg ctgaacttgc tgtatgaggc
     6301 cgatgcggcc taccgcagtg gacttaaggt cgcccccgac catgccgcgc tgcacaccgc
     6361 gcatgccatg gccgagcggg ccctcgggca gctcgcggag gcgcaggcga gcgcgcggcg
     6421 ggcgctcgcg ctgcgtcccg gcgctgcaga cacgctcgcg ttgctcggca gcctcgccat
     6481 cgaccatggt cggttcgatg aggctgaaga attgctgcgc aaagcattgc tcatcgaccc
     6541 ggagctgccg gaggcgctga ccagcctcat ggccgtgcgc agaatgggtc cggcggaggc
     6601 gccgtggcgc gacgccgcgg agcgcgtgct ggcgcgcggc ttgagtgtgg cacacgccat
     6661 caacctgcac catgcgctgg gcaagtacta cgatgacgtc gacgaccgtg atgcggcttt
     6721 cgagcatcat cggctcggca acgaactcgc ccgtcgcagc cgaattcgct acgaccgggc
     6781 ggagtggacg cagcgggtgg cgcggacgat cgcggctttc gatcgaaatg cgctcgcggc
     6841 cttaagttcc tgcggcgttg cctccgaacg tccggtgttc gtggtcggaa tgccgcgctc
     6901 gggcacgagc ctgaccgaac agatcctcgc ctcgcacccc gaggtctacg gcgcaggtga
     6961 gcttctgtac tggatttttg ccgcggacgc cgagcgcgcc gcgaccctcc cgcagcgcgc
     7021 ggcgaagatc atcgagcttg gccgctccta cctggagggg cttgcgttgc aggcgcccga
     7081 tgcgtcccgc gtcgtcgaca agctgcccgt caacttcgcc aacatcggcc tcattcacgc
     7141 ggcgctgccc ggtgcccggt tcattcatct tgagcgaaac gcgctcgaca cgtgcttgtc
     7201 gatctacttt caggggttct ccgcggcgca cccctacgcg accgacttcg gcgaccttgc
     7261 gcattactac catgagtacc ggcgtctgat ggcgcactgg cgcgccgtgt tgccgccgca
     7321 gtccctattc gaggtgcgct atgaagacct ggtcgacgat ccgcaaggct ggagccgccg
     7381 aatgctcgag cacattggac tgccgtggga ttcgcgctgc ttggaattct accgcaccga
     7441 tcggccggtg ttgacggcga gcaattggca ggtgcgacag cccatcggca agggctcact
     7501 cgatcggtgg cgtcgctatg agggctttat cgggccgctg cgcgcggcgc tcggcgaggg
     7561 gtaggcggcg gcggccaaat ttctgcggac agttgttcgg tgaggtgcaa gcgcggtagg
     7621 gtggggcatg ctgaggctgt tgcggttatg gcgcattgcg ggaaaggact tgtccttgct
     7681 gtggttcgcg ctgcgccagc gacatcggcc gatttggctg ttgccggccg tcgtgcttct
     7741 cggcttgtac gcgctcgagc cgctgaattt tgcgattccc ctgctaggag tggttgacga
     7801 cgtcatcttg ctgccgttgg cattgcattt catggccagg tgcttgcccg ctgaaatccg
     7861 ttacggcttt gcgcggcgcg gctaagcggc ggactttcgc ggcttcagaa gaaacaaagc
     7921 gcgacgggca ccacgatacc cactcccagc agccaccagc ccgcgcgttt gcggcggact
     7981 tgcatcagtc ccatccacag gcccaacagc gtcgacgccg tgagcgcaag tgagacgagc
     8041 aaaaagtacc ttttgagcag ataggagccg agcgatgcgg cctcatgatc atcgccaggt
     8101 tgcgggtgtt cggaatgatc gtgatcgtcg tggtcttttt gtgcgaatac ttgatcctta
     8161 tgcacgctgc ttaatttctc g
//
LOCUS       DASWZT010000079         2490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_29630, whole genome shotgun sequence.
ACCESSION   DASWZT010000079 DASWZT010000000
VERSION     DASWZT010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29630"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VGE92_02090"
     CDS             complement(<1..693)
                     /locus_tag="VGE92_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312633.1"
                     /translation="MRASRTVGVAALMLLACSARAGAASPVSCEARSAAALRVQDSTL
                     CVSIDEPRAISQTLVQEWILRSARIVAGYYTRFPAPLVALKVRVEPGSGVRGGRTTND
                     SGLMIQVSVGRDSTAAELAADWVLVHEMVHLALPELGRGHDWLAEGLATYVEGIARAQ
                     SGNREIADVWAESRHSMPLGLPHAGEGGMDQTHTWGRTYWGGALFCLQADVKIREQTG
                     NRIGLQTALQAIL"
     gene            complement(666..1637)
                     /gene="mmuM"
                     /locus_tag="VGE92_02095"
     CDS             complement(666..1637)
                     /gene="mmuM"
                     /locus_tag="VGE92_02095"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF007020.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047150 - betaine-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HEY1312634.1"
                     /translation="MTLSRTILRDPIAAFLALHPLLVLDGALATELERRGADLDDPLW
                     SAKCLLECPRLIKDVHADYFRAGADVATTATYQATFETLARRGIGRDAARQLLCDAIA
                     LAVAARDEFWAVAQNRIARSRPLIAASVGPYGAMLADGSEYRGHYALDAAALADFHRP
                     RLAVLAGAGADLLAFETIPCLREARVLAQLLQEFPGVCAWLSFSCQDGAHNCAGEPIG
                     ACAAQLQEYAQIVALGVNCTPPQYVASLLGRMRAATSKPLVAYPNSGEHYAAAAKRWS
                     GRRAAGEFGDQARIWYAAGARLIGGCCRTGPSDIRSVKQCVHPEPSA"
     gene            1688..>2490
                     /locus_tag="VGE92_02100"
     CDS             1688..>2490
                     /locus_tag="VGE92_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312635.1"
                     /translation="MLTAQTIMNRHSDIFTFLHGDLAIGASNCHIPEEALVGSLVYVS
                     DAGQLAEARRHKPSILIVAAKMAGCTDALLDADSCCFSVQSISMGMAVLLKYFDRKCY
                     RFTQWGERHPTTVVHPDAVIGERSFLGPYCVIGAHARIGNDCLIGAHVVVENDARVGA
                     RTVLHPHVFVGAGCEVGDDCEIHPHTSIGSDGFGYAVDPDGRPRKISHLGNVKIGNGV
                     EIGSNCAVDRATLTSTYIRSGTKLDNICHIAHNCDLGENGFFTAGFMMGG"
BASE COUNT          473 a          805 c          797 g          415 t
ORIGIN      
        1 caatatggct tgcagcgcgg tttgcagtcc gatgcgattg ccggtttgtt cgcgaatttt
       61 cacatcggcc tgcaagcaga acagggcgcc gccccagtag gtccgtcccc aggtatgcgt
      121 ttgatccatg ccgccctcgc ctgcatgcgg caggccgagc ggcatggaat gccgggactc
      181 cgcccaaaca tcggcgatct cgcgattgcc ggattgggcg cgcgcaatac cttcgacata
      241 ggtcgccagg ccctccgcga gccaatcatg tccccgaccg agttcgggca gagcgaggtg
      301 caccatctcg tgcaccaaga cccagtccgc ggcaagctcc gcggccgtcg agtcgcgccc
      361 cacgctcacc tggatcatca accccgaatc gttggtggtg cggccgccgc gcacgccgct
      421 gccgggctcg acacggacct tgagtgccac gagcggcgcc ggaaaacgcg tgtagtaacc
      481 ggccacgatg cgcgccgatc gcaatatcca ctcctgcacc agggtctgcg agatcgcccg
      541 cggctcgtcg atcgatacgc acagagtcga atcctgcacg cgcaacgcgg cggcgctgcg
      601 agcttcgcag cttacgggcg acgcagcgcc cgcgcgcgcg ctgcacgcga gcagcatcag
      661 ggcggctacg ccgacggttc gggatgcacg cattgcttta cactgcgaat gtcggaaggt
      721 cctgtacggc agcagccgcc gatcaagcgt gcgcctgcgg cgtaccaaat gcgagcctgg
      781 tcgccgaact cgcccgcagc gcgtcggccg ctccagcgtt tggccgcggc tgcatagtgc
      841 tcgccggaat tcgggtaagc aacgagcggc ttggacgttg cggcgcgcat gcgcccgagc
      901 agcgatgcga cgtattgcgg cggggtgcaa ttcacgccga gcgcgacgat ctgagcatac
      961 tcttgcagct gcgccgcgca cgcgccgatc ggctcgcccg cgcaattgtg cgcgccgtcc
     1021 tggcatgaaa aactcagcca cgcgcaaacg cccggaaatt cttgcagcaa ttgcgccaac
     1081 acccgcgctt cgcgcagaca cggaatggtt tcgaatgcca ggagatcggc gcccgcgccg
     1141 gcgagcacgg cgagccgcgg ccgatgaaaa tccgccaaag cggcagcatc gagcgcgtag
     1201 tgcccacggt attcggaacc gtccgccagc atcgcgccgt agggcccgac cgacgcggcg
     1261 atgagcggcc gggagcgggc gatgcggttc tgcgcaaccg cccaaaactc atcgcgcgcg
     1321 gcgaccgcca acgcgatcgc atcgcacagc agctggcggg cggcgtcccg cccgatgccg
     1381 cggcgcgcca aggtttcgaa ggttgcctga taggtcgcgg tggtggcgac atcggcgccg
     1441 gcgcgaaagt agtccgcgtg cacgtccttg atcagccgcg ggcattcgag caggcacttt
     1501 gcggaccaaa gtggatcgtc caaatcggcg ccgcggcgct ccaattcggt tgccaacgcg
     1561 ccgtccagca ccagcaaagg atgcaacgca aggaaagcgg cgatcggatc gcgcaggatt
     1621 gttcgcgaaa gagtcaattt gcgctcatac tctgcgggct ctcacccatg caacggtaag
     1681 ggacgctatg ctcaccgcgc aaaccatcat gaatcggcat tccgatatct ttacattttt
     1741 gcacggtgac ctggcgatcg gcgcgagcaa ttgccacatc ccggaagagg cgctggtggg
     1801 cagcctcgtg tacgtgagcg acgccgggca gctggcggaa gcgcggcgcc acaagccgtc
     1861 gatactgatc gttgccgcga aaatggccgg ctgcacggat gctctgttgg atgccgacag
     1921 ctgctgcttt tcggtgcaga gcatatcgat gggaatggcg gtgctgctca agtacttcga
     1981 ccgcaaatgt tatcgtttca cgcagtgggg cgaacgtcat ccgaccacgg tggtgcatcc
     2041 cgatgccgtg atcggcgaac gcagtttcct cggcccgtat tgcgtgatcg gagcgcatgc
     2101 ccgtatcggc aacgactgcc tgatcggtgc acacgtagtg gtcgaaaacg acgcccgagt
     2161 cggcgcgaga accgtactgc acccgcatgt tttcgtcggc gccggctgcg aagtgggcga
     2221 tgactgcgag attcatccgc ataccagcat cggcagcgac ggattcggct acgccgtcga
     2281 tccggacggc aggccgcgaa aaatctcgca cttgggcaac gtcaaaatcg gcaacggcgt
     2341 cgagatcggc agcaactgcg cggtcgaccg tgccaccctg acgtcgacct acattcgatc
     2401 gggtaccaag ctcgacaaca tttgtcatat tgcgcacaac tgcgacctgg gcgagaacgg
     2461 tttttttacg gcgggcttca tgatgggggg
//
LOCUS       DASWZT010000080         1666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_73168, whole genome shotgun sequence.
ACCESSION   DASWZT010000080 DASWZT010000000
VERSION     DASWZT010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73168"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..764
                     /locus_tag="VGE92_02105"
     CDS             183..764
                     /locus_tag="VGE92_02105"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY1312636.1"
                     /translation="MINANSSGRVPAPEPSVTRPEPREPRAEPREPRAEPRAPQPRAA
                     EARAPEVRVPEPAARASVLGPTLRFRGELSAQEDLIVQGSIEGSITHTQSLTIGTDGS
                     MKGDIRARVIVIDGKVEGDLYATESVSIRATAKVKGNVFAPRVGITDGAFFQGQVEMQ
                     PSGAAVQEHSARLRQASQAPLEGAVVDQMLGAS"
     gene            complement(789..1604)
                     /locus_tag="VGE92_02110"
     CDS             complement(789..1604)
                     /locus_tag="VGE92_02110"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY1312637.1"
                     /translation="MNQKSLMAGLLLASHAFGQGLELPRESAVPGGVKILRLETTGPV
                     MPYVDTDGNRALVIQDGSHWVAIIGIPLSAPLGVRPVIVRGADRREEIDFTVGDKQYT
                     SQSLKVAPRQVNPSPADLTRINAEKIRIAHALSHWSDLAPESLRLPQPVPGPRSSSFG
                     SRRIFNGEARNPHSGMDIAAPTGTPVRVPIAGTVVDTGEYFFNGNTVFVDHGRGLISM
                     YCHLSSIDVQPGQRVAAGARLGAVGMTGRATGPHLHWGLNLNHAWVDPELFLP"
BASE COUNT          305 a          527 c          547 g          287 t
ORIGIN      
        1 atctacgccc cttccgtgag catcatggag ggcgcgagtt tcagcggcaa tatcgacatg
       61 ggcggcaaga aggcggctca gctcggtaaa caagatgcct caccggtacg gacgcccaag
      121 gtttcgggcg gtgcggcgta acgatgtcct ggtttaaaaa acccctcgac ggagacgatc
      181 ccatgatcaa tgccaattca agcggccgtg tgcctgcacc cgaaccgagc gtgacgcgcc
      241 ccgagccgcg tgagccgcgc gccgaaccgc gcgagccgcg tgcggagccg cgcgcgccgc
      301 aaccgcgcgc tgccgaagcg cgcgcgcccg aagtgcgtgt cccggaaccc gctgcgcgcg
      361 cctcggtctt ggggccgacg ctgcgattcc gaggtgagct atcggcgcaa gaggacttga
      421 tcgttcaagg cagcattgaa ggttcgatca cgcataccca gagcctgacc atcgggaccg
      481 atggctcgat gaaaggcgac attcgcgcac gggtgatcgt gatcgacggc aaggtcgaag
      541 gcgacctgta tgccaccgaa tccgtaagca tccgcgccac ggcgaaggtc aaagggaatg
      601 tctttgcgcc gcgcgtcggc atcaccgatg gtgcgttttt ccagggtcag gtcgaaatgc
      661 agccctccgg cgccgcggtg caggaacata gcgcccggct gcgtcaggcc tcgcaggcgc
      721 cgctcgaagg agccgtggtc gatcagatgt tgggcgcaag ctagtagcgg tcgcggggcc
      781 gtattgcctc agggcaggaa caactccgga tcgacccacg cgtggttcag attcaatccc
      841 cagtgcagat ggggcccggt cgcgcgcccg gtcatcccga cggcgccgag gcgcgcgccc
      901 gcggctacgc gttgtccggg ttgcacgtcg atcgagctca agtggcagta catactgatc
      961 aggccacggc cgtgatcgac gaagacggtg ttgccgttga agaaatactc tcccgtgtcg
     1021 accacagtgc ccgcgatcgg cacccgtacg ggagtacccg tgggtgccgc gatgtccatg
     1081 ccactgtgcg gattgcgggc ttcgccgttg aagattctgc gcgagccgaa tgagctgctg
     1141 cgtggaccgg gcaccggttg cggcaagcgc aacgattccg gtgcaagatc gctccagtgg
     1201 ctcaacgcat gggcgatgcg gatcttctcc gcgttgatgc gcgtcaggtc tgcaggcgat
     1261 gggttgactt ggcgcggtgc aactttgagc gactggctgg tgtattgctt gtcgcccacg
     1321 gtgaaatcga tttcctctcg gcgatcggcg ccgcgcacga tcacgggccg tacgcccaac
     1381 ggtgccgaca aggggatgcc gatgatggcc acccaatgcg agccgtcctg gatcaccagt
     1441 gcccgatttc catcggtgtc gacgtagggc atcacagggc ccgtcgtctc cagccgcagg
     1501 atcttcactc cgcctggtac cgcgctctcg cgcggcaatt caaggccctg tccgaacgcg
     1561 tgagaggcga gcagcaggcc cgccatcaat gatttttgat tcacgaagca ccctcgactc
     1621 gggcacgaac ccggcctcgt gcgagccgcg cttcgatcat ggagcc
//
LOCUS       DASWZT010000081         6002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_39432, whole genome shotgun sequence.
ACCESSION   DASWZT010000081 DASWZT010000000
VERSION     DASWZT010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39432"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VGE92_02115"
     CDS             complement(<1..187)
                     /locus_tag="VGE92_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312638.1"
                     /translation="MPTGPLTFTRGAAACVAVAVGLAACASTPIPNEKIAVAKASVAN
                     AEQSGAPELAPVEMAAAR"
     gene            complement(458..835)
                     /locus_tag="VGE92_02120"
     CDS             complement(458..835)
                     /locus_tag="VGE92_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1312639.1"
                     /translation="MRVLLVEDSKVLTERLTEAIRQIPEVELIGTADTEATAVASAKR
                     DAIDVIILDLHLKQGTGFGVMRALAMAQLKPRIIVLTNYDLPEYKNAAIALGATHFLD
                     KARDYGRLPEVLHEICEAHQSKH"
     gene            complement(964..1596)
                     /locus_tag="VGE92_02125"
     CDS             complement(964..1596)
                     /locus_tag="VGE92_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY1312640.1"
                     /translation="MSRVLIADDHAVVRAGFKQFLEADSSINAVGEAASGNATLDMLR
                     EQEWDLVLLDIHMPDRSGLDILRHIQTGHPGVRVLVMSGLPEQQYAVNVLRAGASGYL
                     SKDSAPEELMKAVRTVLAGRRYVSAALAEILVADLDGDADKPLHARLSTREFQIFCKL
                     AAGRGVSDIANELSLSVKTVSTYRSRILEKMSFTANADITSYALRNGLIQ"
     gene            complement(1621..2622)
                     /locus_tag="VGE92_02130"
     CDS             complement(1621..2622)
                     /locus_tag="VGE92_02130"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY1312641.1"
                     /translation="MNSVSCDFSEQAAPTGAAGRLLGESAAMQDVYRLIERVGPTEAS
                     VLLTGESGSGKELAAQSIHACSRRRSGPFVAINCGAIPAGLIEAELFGYEKGGFTGAA
                     RAHAGVFERAQGGTLLLDEVTEMPLEMQTRLLRVLETKKFYRVGASTELASDVRVIAS
                     TNRCPLQAVQSGQLREDLLYRLAVFPIALPALRSRGDDVQLLADHFLAELNAERRTQK
                     RLSALARMTLKQHSWPGNVRELKNCIERAFILGDTVIELAPLIPGTTDGGQDGDRERL
                     SIRVGSRIYDMERSLIEATLDYFKGNKRRAATALGCSLKTLYNKLNGYSQRQECANS"
     gene            complement(2749..3060)
                     /locus_tag="VGE92_02135"
     CDS             complement(2749..3060)
                     /locus_tag="VGE92_02135"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEY1312642.1"
                     /translation="MKRVFRAASLLQVAHARNVLLTAGIQSELRNQYLAGAFGDLPML
                     ETWPQLFVDDADEQMALSALARAAAAPSGAPWTCENCSEVLEPQFTSCWRCGSNVSDK
                     H"
     gene            complement(3091..3885)
                     /gene="trpA"
                     /locus_tag="VGE92_02140"
     CDS             complement(3091..3885)
                     /gene="trpA"
                     /locus_tag="VGE92_02140"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEY1312643.1"
                     /translation="MTRGAVPGESIASAIRAAAAEGRPALVGFLTAGFPTRRDFMANL
                     AAVAAVCDVVEVGVPFSDPMADGTTVQRASFVALRDGVTLPWIFEQLKSLPRRHAAPL
                     LLMSYLNPLLSLGLDALPRTAAAAGVAGFIVPDLPFEESDDLKAALEHEGLALVQMVT
                     PVTPPQRLALLCGQAKGFVYAVTMTGTTGGGKGSSQVPPDVLEYMRRVKACSRVPVCA
                     GFGIRSREQVAQFTGHVDGVVVGSALVETLERGEDVGAFLRSLRAA"
     gene            complement(3882..5105)
                     /gene="trpB"
                     /locus_tag="VGE92_02145"
     CDS             complement(3882..5105)
                     /gene="trpB"
                     /locus_tag="VGE92_02145"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018927237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEY1312644.1"
                     /translation="MNSPVRTASKTADVPDARGRYGAFGGRYVPETLVPAIDRLQAGV
                     DRYLDDPEFQAEFAYELKTWVGRPTAVSHASTLSKRWGADVWLKREDLAHTGAHKINN
                     AVGQTLLAKRLGAKRIVAETGAGQHGVASAAAAARLGIPCVVYMGAVDVERQAPNVGR
                     MQLLGATVVPVTGGDQTLRAAIDEALRDWVSDPDGTYYCLGSAVGPHPYPYLVRELQA
                     VIGREARAQMLELAGGLPDVVVACVGGGSNAIGLFHPFLEDAVVTLIGVEAGGRGTGL
                     GDNAASLTFGTPGVLQGSYTLILQDEFGQVRETHSVSAGLDYSGVGPEHAYLMKSGRV
                     AYESATDEEALDALAECAKFEGILPAIESAHAFAGARRYARAHPGTRVLVGCSGRGDK
                     DMPILQRTLLKDSGQ"
     gene            complement(5102..5722)
                     /locus_tag="VGE92_02150"
     CDS             complement(5102..5722)
                     /locus_tag="VGE92_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEY1312645.1"
                     /translation="MATLGAMWIKICGITSADAVAAAAEAQVDAIGFVFAPSPRQVTP
                     GQAAQLAALAPPGILRIAVAQHPLQMKVDEICRILKPDYFQTDVEDLRELKIPAHIKV
                     LPVVRFGRKTPSPLPALMLFEGPSSGIGELADWGRAAELARQTAVILAGGLSAQNVAE
                     AIQVVRPYGVDVSSGVEEVPGVKDPAKILEFVRATRLAAASLESDS"
     gene            complement(5723..>6002)
                     /locus_tag="VGE92_02155"
     CDS             complement(5723..>6002)
                     /locus_tag="VGE92_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312646.1"
                     /translation="TAAPARQPVLIGVNCRDLQTLAVVTQRFATLAPRLPKGRPAVAE
                     SGVACAADARRMLELGYRLALIGTALMSREDPAALLREIRDATRTVQT"
BASE COUNT         1063 a         1997 c         1840 g         1102 t
ORIGIN      
        1 cgcgagccgc cgccatttcg acgggtgcaa gttccggggc gcccgactgc tcggcgttag
       61 ccaccgatgc cttggcgact gcaatttttt cgttgggtat gggagtactc gcgcaggcag
      121 caagcccgac cgcgacagcg acgcatgcag cggcaccccg tgtgaatgtt aatggcccgg
      181 taggcatgtc tctaactcct gtgaagcatt ttcgatccgt ttcggcatgc acaataaact
      241 gcgccgcgcg gcgtggatgt gggcgcagcg cgtgagcgcg tgtcgggaac cggccgcaag
      301 ggttgtaggc gttacggcag tcggggtggt ccgggcaggc gccgcgcgcg atacgactgc
      361 gcgcacgcgg cagcgctggc tgcgctggct gcgctggctg cgctggctgc gctggctgcg
      421 ctcaaatcat caagcgaggt tgcggtcgcg gtggcgctca gtgtttcgac tgatgcgctt
      481 cgcagatttc gtgcagcact tccggcagcc ggccgtagtc gcgcgcttta tccaagaaat
      541 gagttgcgcc gagcgcaatt gcggcattct tgtattccgg caaatcgtag ttcgtgagga
      601 cgatgattcg cggcttcaac tgcgccatgg ccaatgcgcg catcacgccg aagcccgtgc
      661 cctgtttcaa atgcagatcc aggatgatca catcgatcgc atcgcgcttc gccgaggcga
      721 ccgcggtcgc ctccgtatcc gcggtgccga tcagttccac ttcgggaatc tgccgtatcg
      781 cctcggtcaa tcgctccgtc agcaccttcg agtcttcgac tagcaggact ctcaaggact
      841 gagccattga cactgcgccc ataatattgt tgaacatctc agtcgcagtt tagcgtcatc
      901 taccgtacgc cgcccagacc gcgtggacag gttcaaatgt aggatatttc tcacaaggca
      961 ggcttattgg atcaggccgt tgcgcagcgc ataggacgta atatcggcgt tggccgtgaa
     1021 gctcattttc tccaagatcc ggctgcgata ggtgcttacg gtcttcacac tgagactgag
     1081 ttcattcgcg atatcggaaa caccgcgccc ggcagccaat ttacaaaata tttggaattc
     1141 gcgggtcgag aggcgcgcat gcaagggttt gtccgcatcg ccgtccaaat ccgcgaccag
     1201 gatctcggcc agcgcggcgc tgacataacg ccggccggcc aataccgtgc gcaccgcctt
     1261 catcaactcc tcgggggcgc tgtctttgga gagataaccg ctggctccgg cacgcagcac
     1321 attgacggcg tattgctgtt cgggcaggcc gctcatcacc aacacgcgaa cgccggggtg
     1381 tcctgtttga atatggcgca agatgtcgag gccgctgcga tccggcatgt ggatgtcgag
     1441 cagcacgagg tcccactcct gttcgcgcag catgtccaag gtcgcgttgc cgctggcagc
     1501 ctcgccgacc gcattgatgc tcgagtcggc ttcgaggaat tgcttgaacc cggcgcgaac
     1561 tacggcgtga tcgtccgcga tcagtactct tgacacgatt gaatcccctc ttggtgggcg
     1621 ctaggagttc gcgcactctt ggcgttgtga atatccattg agcttgttat acaacgtctt
     1681 gagactgcag cccagcgcag tcgccgcgcg gcgcttgttg cccttgaagt aatccagggt
     1741 tgcctcgatc agcgagcgct ccatgtcgta gatgcgcgag ccgacgcgga tgctgagccg
     1801 ctcgcggtcg ccgtcttgcc caccgtccgt cgtccccggg atcagcggtg ccaattcgat
     1861 cacggtgtcg cctaagatga aggcgcgctc gatgcagttc ttcagctcgc gcacgttgcc
     1921 gggccaactg tgctgtttga gtgtcatgcg cgcgagcgcg gacagccgct tttgcgtgcg
     1981 ccgctcggcg ttgagttcgg ccaagaaatg atccgccagc agctgcacat cgtcgccgcg
     2041 gctgcgcagc gcgggcagcg caatcggaaa tacggccagg cggtacagca gatcctcgcg
     2101 caactggccg ctctgcaccg cttgcagggg gcagcggttg gtcgaagcaa tcacgcgcac
     2161 atcggatgcg agttcggtac tcgctccaac ccggtagaat tttttggtct cgagcacgcg
     2221 caacagccgc gtctgcatct cgagcggcat ctcggtgact tcgtcgagca gcagcgtccc
     2281 gccttgcgcg cgctcgaaga ctccggcgtg ggcgcgcgcg gcgccggtga agccgccttt
     2341 ctcgtagccg aacagttcag cttcgatgag ccccgcgggg atcgccccgc agttgattgc
     2401 gacgaacggg ccgctgcgcc gccggctgca ggcgtggatc gactgcgcgg ccaactcctt
     2461 gccggaaccg ctttccccgg tcaagagcac gctcgcttcg gtgggcccga cacgctcgat
     2521 caaccggtac acgtcttgca tggcggctga ttcgccgagt agtcgacctg ctgcgcccgt
     2581 tggcgcggcc tgctcggaaa aatcgcagct gacactgttc atgtaaagtt cccctccgga
     2641 tcgcgccgtg taatcgttac aactggacgc aagatttgta acccgcgccg cccgcgcgct
     2701 accaggggta gaagctgaac ctcgcctcag atttcgagcg attctttgtc agtgtttgtc
     2761 cgacacattc gatccacaac gccaacaact ggtgaattgc ggctcgagaa cttcgctgca
     2821 attctcgcaa gtccagggcg cgccgctcgg agccgcagcg gcccgcgcca gggcggagag
     2881 cgccatttgt tcatcggcat cgtcgacaaa caattgcggc caagtctcca gcatcggcaa
     2941 atcgccgaac gcgccggcca gatattgatt gcgcaactcg ctttggatac cggcggtcag
     3001 gagcacattg cgtgcatggg ccacctgcag gagcgacgcg gcgcgaaata cccgcttcat
     3061 gagccggcat gccccgcggc gcgcgtcata tcaagccgca cgcaacgatc gcaggaatgc
     3121 gccgacatct tcgccccgct ccagggtttc aaccaatgcg gagcccacca cgacaccgtc
     3181 gacgtgcccc gtgaattgcg ccacttgttc gcgcgagcgg atgccgaacc ccgcgcacac
     3241 cggtacgcgc gaacaggctt tcacgcggcg catgtactcg agcacatcgg gcggcacctg
     3301 gctcgagccc tttccgcccc cggtggtacc cgtcatggtc accgcataaa caaagccttt
     3361 ggcctggccg cacagcagtg ccaggcgttg cggtggcgtg accggcgtca ccatctgcac
     3421 cagcgcaaga ccctcgtgct cgagtgccgc cttgaggtca tcgctctcct cgaacggcag
     3481 gtcaggcacg atgaaaccgg cgaccccggc agccgccgcc gtgcgcggca gcgcgtcgag
     3541 tcccaggctc agcagcgggt tcaaataact catcaaaagc aggggggcgg cgtgtcttcg
     3601 cggcagggat ttcaattgtt cgaagatcca cggcaaggtc acgccgtctc tcaaggcgac
     3661 gaaactcgca cgctgaacgg tggttccatc ggccatggga tcgctgaacg gcacgccgac
     3721 ctcgaccacg tcgcaaacgg cggcaacggc ggccagattc gccatgaaat cccgtcgtgt
     3781 cggaaagccc gccgtcagaa accccaccag cgccggccgc ccctcggctg ccgctgctcg
     3841 aatggcggag gcgatgctct cgcccggcac cgctccgcgc gtcattggcc gctatccttc
     3901 aagagcgtgc gctgcaagat gggcatgtcc ttgtcgcccc tgccggaaca gccgaccagg
     3961 acgcgcgtcc cgggatgcgc ccgggcgtaa cggcgtgcac cggcaaatgc atgggcgctc
     4021 tcgatggccg gcaagatgcc ctcgaacttg gcgcactcgg cgagcgcgtc cagggcttcc
     4081 tcgtcggtcg ccgactcata ggccactcgt ccgctcttca tcaggtaggc gtgctccgga
     4141 ccgacgccgg agtaatcgag gcctgccgat accgaatggg tttcccggac ctgcccgaac
     4201 tcgtcctgga gtatcagcgt atagctgcct tgcaacacac cgggcgtccc gaaagtcaaa
     4261 gaagctgcat tgtcgccgag gcccgtgccg cgcccgccgg cttccacgcc gatcagcgtc
     4321 accacggcat cctccaagaa gggatggaac aagccgatgg cattcgagcc gccgccgaca
     4381 caggccacca cgacatccgg caaacccccg gccaactcca acatctgcgc gcgcgcttcg
     4441 cggccgatca cggcctgcag ttcgcgcacc agatacggat acggatgcgg tcccaccgcc
     4501 gaacccaggc agtaatacgt gccatcggga tccgataccc aatcgcgcaa cgcttcgtcg
     4561 atggcggcgc gcagcgtttg atcgccgccc gtgacgggca ccacggtcgc gcccaacagc
     4621 tgcatccgcc cgacgtttgg tgcttgccgt tccacgtcga ccgcccccat gtagaccacg
     4681 cagggtattc ccagccgcgc tgccgcggca gcgctcgcga cgccatgctg acccgctccg
     4741 gtctccgcga cgatgcgttt tgcgccgagc ctctttgcca acaaggtttg gcccacggca
     4801 ttgttgatct tgtgcgcgcc ggtatgcgcc agatcttcgc gtttcagcca gacatcggct
     4861 ccccatcgct tgctcaacgt cgatgcatgc gaaacggcgg tcgggcgccc cacccaggtc
     4921 ttgagctcgt aggcgaactc ggcctgaaat tccggatcat cgagatagcg gtcaaccccc
     4981 gcctgcagcc ggtcgattgc cggcaccaag gtctcgggaa catagcgtcc gccgaacgct
     5041 ccgtaccggc cgcgggcatc gggaacatcg gccgttttgc tcgcagtgcg cactggtgaa
     5101 ttcaagaatc gctctccagg ctcgccgccg cgaggcgggt cgcgcgcacg aactcgagaa
     5161 ttttcgcggg gtccttgacg ccgggtacct cttcgacgcc gctgctcaca tccacgccgt
     5221 aagggcgcac cacttgaatc gcctcggcca cgttctgcgc ggacaacccg ccggcgagaa
     5281 tcaccgcggt ctgccgcgcc agttccgccg cgcggcccca gtcggcgagt tctccgatgc
     5341 cgctcgacgg tccctcgaac aacatcagcg ccggcagcgg actcggtgtc ttacggccga
     5401 atcgcacgac cggcagcacc ttgatgtgcg cgggaatctt gagctcgcgc aaatcttcga
     5461 cgtcggtctg aaaatagtcc ggtttgagaa tccggcagat ttcgtctacc ttcatctgca
     5521 gcgggtgctg cgcgacggcg atgcgcagaa tccccggcgg cgccagcgcc gccagttgcg
     5581 ccgcctgccc gggcgtcacc tggcgcggac tcggagcgaa cacgaagccg atggcgtcga
     5641 cctgcgcctc ggcggccgcc gccaccgcgt cggcactggt gatgccgcag atcttgatcc
     5701 acatggcgcc gagtgtagcc atttaagttt gcactgtccg cgtcgcatcg cgaatttcgc
     5761 gaagcagcgc tgcgggatct tcgcgcgaca tgagcgcggt gccgatcaag gcgagtcgat
     5821 agccgagttc gagcatgcgg cgtgcgtccg cggcgcaagc gacgccgctc tcggcaaccg
     5881 cggggcgccc cttgggcaag cgcggtgcga gcgtcgcaaa tctctgcgtg accaccgcga
     5941 gtgtttgcaa gtcgcggcag ttgactccga tcaagacggg ctggcgcgcc ggcgccgccg
     6001 tg
//
LOCUS       DASWZT010000082         4893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_15136, whole genome shotgun sequence.
ACCESSION   DASWZT010000082 DASWZT010000000
VERSION     DASWZT010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15136"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="VGE92_02160"
     CDS             <1..962
                     /locus_tag="VGE92_02160"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY1312647.1"
                     /translation="EVFVRREVIDAAQLRALCVTSNRAGAVQAASHLGAIAVTGTALW
                     LTLGTWWALPVFAAHGVLLNFLYAGQHELSHWTVFRTAWLNEWLGRVFGFVLFYPRTF
                     DQVQHIAHHRFTQDWVRDGELARERYTLASYLLWMSGVTYWYTRWRRIVRFSFGAVTE
                     PYLPARRRAEFIREARWHLAGYASIAAISLALRSYAVVILWLAPMLAMKGVHQLQNTI
                     EHLGLPHQDNVLANTRSTRTNAAMRWLGWQMQYHTAHHAFPGVPFHRLHALNEAIFTA
                     RAAAPPSMTYLGFQIAVLRAFAHGKTEADYPDDSTWIADRPHG"
     gene            955..1812
                     /locus_tag="VGE92_02165"
     CDS             955..1812
                     /locus_tag="VGE92_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312648.1"
                     /translation="MGSRLLVYQALWAMDRLSVPEASIAEKFDRVREAGFQGMAIDLG
                     ALTMEQAQATVPHFARTGLAGGLTAFPTSIEALRPALELAHRIAAPFVVVIGQEMPVR
                     LADMIPVIEGWLRVAEQERMPIQFETHRNCITNDLFTTVQLLDALPQMRLAADLSHYV
                     VDREMPCPPPPQLQRLIAQVLERSDSLQGRVAARGQIQIALGFPQNAKWVALFRDWWR
                     QGFRAWRARHRGDDSAMVFLCELGPPDYALTDADGREFSDRWSEALLLAQWARDLWSS
                     AAPDAATRP"
     gene            complement(1799..2221)
                     /locus_tag="VGE92_02170"
     CDS             complement(1799..2221)
                     /locus_tag="VGE92_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003110349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY1312649.1"
                     /translation="MNKDWRLFALGSALFAALTAIFGKVGVAEIDSDLATFVRTIVIL
                     MVVALLLSVRHEWTRPDALSATGMVFLVLSGIATGLSWLCYYRALQLGPASRVAPIDK
                     LSVIFVVLFAVCFLGERFTWKVGLGGLLVTVGAALMAL"
     gene            2359..4176
                     /locus_tag="VGE92_02175"
     CDS             2359..4176
                     /locus_tag="VGE92_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY1312650.1"
                     /translation="MSLPIENYALIGDCHTAALVGRDGSIDWLCLPRFDSGACFAALL
                     GSAEHGRWLLAPVAQADRVTRSYRGDSLILETQFDTAEGSVRIIDFMPLSNKRWDVVR
                     IVEGLSGRVALRMELRVRFDYGSIVPWVRRAGQKLLITAGPDTLELTTSVAVKGENMH
                     SVAEFCVSAGGRESFVLNYRPSHVEGQALIDADQALAATAGQWHEWSKQCVYQGRWRD
                     CVMRSLLTLKALTYKPTGGLIAAPTTSLPEQLGGVRNWDYRYCWLRDATFTLNALLLA
                     GYENEAADWREWLLRAIAGSPDDLQILYGVTGVRRLDECEIPWLPGYQGAAPVRIGNA
                     AAKQFQLDVYGEVMDTLHLARAAGLPPESEAWRVQVALLQFLDSHWQQPDEGLWEVRG
                     PRRHFTHSKIMAWVAFDRAIKDAEQDGLDGPIERWRQLRDTIHAEVCERGFDARRNTF
                     VQSYESPHLDASLLLIPQVGFLPAEDARVVGTIRAIEDNLMIDGLVLRYSTATNVDAL
                     PPGEGAFLPCSFWLADSYLLTGRRAEAEALFERLRSLANDVGLFAEEYDPRGRRMLGN
                     FPQALSHMALVNTARLLSMPEHEARGASARGERPAAVAQ"
     gene            4397..4639
                     /locus_tag="VGE92_02180"
     CDS             4397..4639
                     /locus_tag="VGE92_02180"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY1312651.1"
                     /translation="MSTDTNHHFVKDSVITTKVKSKLAAKNLTTLTRIKVDTDENGVV
                     WLSGRAPTQDAVDMATMIAKDTDGVKSVHNDIAIQP"
     gene            complement(4685..>4893)
                     /locus_tag="VGE92_02185"
     CDS             complement(4685..>4893)
                     /locus_tag="VGE92_02185"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEY1312652.1"
                     /translation="VDVLQVSVRQGEGASIEAHWRIVDPHSGKDVVGGEVFRAPLAHD
                     DYAAVAQALSACLSLLADRLIAQM"
BASE COUNT          932 a         1474 c         1545 g          942 t
ORIGIN      
        1 acgaagtttt cgtgcggcgc gaagtaatcg atgccgctca actgcgcgcc ttgtgcgtca
       61 catcgaatcg agcgggcgcc gtccaggcgg cctcccattt aggggctatc gccgtcaccg
      121 gcacggcgct ctggctcacg ctcggcacgt ggtgggcgct gcccgttttt gccgcgcacg
      181 gcgtgctgct caatttcttg tacgccggac agcacgaact cagccactgg acggtgtttc
      241 gcacggcctg gctgaacgag tggctgggcc gcgtcttcgg cttcgtgttg ttctatcctc
      301 gtacattcga ccaagtgcag cacattgcgc atcaccgttt cacccaggac tgggtgcgcg
      361 acggcgagct ggcgcgagag cgctatacgc tggcctcgta cctgctgtgg atgtcgggag
      421 tcacttactg gtacacgcgc tggcgtcgga tcgtgcgctt ttcattcggt gccgtcacag
      481 aaccttacct gcctgccaga cggcgtgccg aatttatccg tgaagcgcgc tggcatctgg
      541 cggggtacgc atcgatcgca gcgatttcgc tcgcgctgcg cagctacgcc gtcgtgattc
      601 tctggttggc gccgatgctg gcgatgaaag gcgttcacca gctgcagaac acgatcgagc
      661 atctcgggct tccgcaccaa gacaatgtgc tcgccaacac gcgcagtacg cgcaccaacg
      721 cggcgatgcg ctggcttggc tggcagatgc agtaccacac cgcgcatcat gcgtttccgg
      781 gagttccttt ccatcgactc catgcgctga acgaggcgat tttcacggcg cgcgccgcag
      841 cgccgccgtc catgacatat ctgggttttc aaatcgcggt gctgcgtgct ttcgcgcacg
      901 gcaaaaccga ggcagattat cccgacgaca gcacgtggat cgccgatcgg ccgcatgggt
      961 agccgtcttc tggtctatca ggctttgtgg gcgatggatc ggctgagcgt accggaggcg
     1021 tcgattgcgg agaaattcga ccgggttcgc gaggctggat ttcaaggcat ggcgatcgat
     1081 ctgggcgctc ttaccatgga gcaagcacag gcgaccgtac cgcatttcgc gcgcaccgga
     1141 ttggccggcg gtctgacggc atttccgacg tcgatcgaag cattgcggcc ggcgctcgag
     1201 ctcgcgcatc ggatcgcggc gccgtttgtg gtggtgatcg gccaagaaat gccggttcgc
     1261 ctcgccgaca tgatcccggt catcgaaggc tggctgcgcg tggcggagca ggagcggatg
     1321 ccgatccaat tcgaaacaca ccgcaactgc atcaccaacg atctgttcac caccgtgcaa
     1381 ctgctcgacg cactgccgca gatgcgcctt gccgccgatc tgtcgcatta tgtggtcgac
     1441 cgcgagatgc cttgtccgcc gccgccgcaa ctgcaacgct tgatcgccca agtgctggag
     1501 cgttccgact cgctgcaagg gcgggtcgcg gcgcgcggcc aaattcaaat cgcgctcggc
     1561 tttccacaga acgcgaaatg ggtggcgcta ttccgcgatt ggtggcggca agggttccgc
     1621 gcgtggcgcg cgcggcaccg cggcgacgat tcggccatgg ttttcttgtg cgaattaggg
     1681 ccgcccgact atgcgctcac cgacgccgac ggccgggagt tctcggatcg atggagcgaa
     1741 gcactgcttc tcgcgcagtg ggcgcgcgat ctatggtcga gcgccgcgcc cgacgcggct
     1801 acaaggccat gagtgccgcg ccgaccgtca ccaacagacc gcccagcccg accttccagg
     1861 taaaccgctc gcccaagaag cacacggcga acaacaccac gaagattacg ctcagcttat
     1921 cgatcggggc gacacgcgag gcaggaccca attgcagcgc ccgatagtag caaagccacg
     1981 ataaaccggt cgcgatccct gataaaacca gaaacaccat gcccgtcgcg gacaacgcat
     2041 cggggcgcgt ccactcgtga cgaaccgaga gcaacagcgc gacaaccatt aaaatgacga
     2101 tcgttctcac gaacgtggca agatccgagt cgatttccgc cacgccgacc ttgccaaaga
     2161 tggccgtcaa cgcggcaaac agggccgatc cgagcgcaaa tagccgccaa tctttgttca
     2221 tgcgagccgc tccgcaccag cgagcaggct aaagtacctg aatatgttcc atgtaggaaa
     2281 ctaccgacgc ggcaacgcgg gccgccaagg tattggcagg gaatcgggtt gggtaatctt
     2341 gggacctgga gcgcacgcat gtctttgccg atcgagaact atgccctaat cggcgactgc
     2401 cacactgcgg ctctcgtcgg cagggacggc tcgattgatt ggctgtgttt gccgcggttc
     2461 gactcaggcg catgtttcgc ggcgctgttg ggcagcgccg agcacggccg ctggctgttg
     2521 gccccggtgg cgcaggcgga tcgtgtgacc cgcagctacc gtggcgacag tctgattctc
     2581 gagacccaat tcgataccgc agaggggagc gttcggatca tcgacttcat gccgctgtcg
     2641 aataagcgct gggatgtcgt tcgcatcgtc gagggcttga gcggccgggt cgcgttgcgc
     2701 atggaactgc gcgtgcgatt cgactacggt tccattgtgc cgtgggttcg ccgtgcgggg
     2761 cagaagttgt tgatcaccgc cggtcccgac accttggagt tgacgacttc ggtcgccgtc
     2821 aagggcgaga acatgcacag cgtcgcggag ttctgtgtga gtgcgggcgg gcgcgagtct
     2881 ttcgtgctca attatcgtcc atcgcacgtc gaggggcagg cgttgatcga tgcggatcaa
     2941 gcactcgccg cgaccgcagg tcagtggcat gaatggtcga agcagtgcgt ttatcagggt
     3001 cgctggcgcg actgcgtgat gcgctcgttg ctcacgttga aagcactcac ctacaagccg
     3061 acgggcggct tgattgccgc tcccaccaca tcactgccgg agcaattggg aggagtgcga
     3121 aactgggatt atcgttattg ctggctgcgc gacgcgacct ttactctaaa tgcgctgctt
     3181 cttgcgggct acgagaacga ggccgccgac tggcgcgagt ggctgctgcg cgcgatcgcc
     3241 ggctcgcccg acgacttgca aattctctac ggggtgacgg gcgtccgacg tctcgacgaa
     3301 tgcgaaattc cgtggctgcc gggttatcaa ggggcggctc cggtgcgcat cgggaatgca
     3361 gcggccaaac agttccagct ggacgtttat ggcgaagtca tggatacgct gcatctggct
     3421 cgggctgccg gcttaccacc ggagtcggaa gcttggagag tccaagttgc tttgttgcaa
     3481 ttcctcgatt cgcattggca acaacccgat gagggtctgt gggaggtgcg cggaccgcgg
     3541 cggcatttca cgcattcgaa gatcatggcc tgggtggcat tcgatcgcgc gatcaaagat
     3601 gcggaacaag acggtctcga cgggccaatc gagcgctggc ggcagctgcg cgatacgatt
     3661 catgccgaag tctgcgaacg cggcttcgac gcacggcgca acacctttgt gcaatcctat
     3721 gaatccccgc acctcgacgc gagtctactg ctgattccgc aggtgggatt tctgcctgcc
     3781 gaagacgcgc gagtggtcgg gaccatccgc gccatcgaag ataacttgat gatcgacggg
     3841 ttggtgctgc gctactccac cgccacgaac gtcgatgcac tgccgcccgg cgagggggca
     3901 ttcctgccgt gttcattctg gctcgcagat agctatcttt tgaccgggcg ccgcgccgag
     3961 gcggaggcat tgttcgagcg gctgcgctcg ctggccaacg atgtcggtct tttcgccgaa
     4021 gaatacgacc cacgcggccg gcgcatgctg ggcaattttc cgcaagcgct gtcgcacatg
     4081 gcgctggtca acacggcgcg gcttttgtcc atgccggaac acgaggcgcg cggcgccagc
     4141 gcccgcggcg agcggccggc ggcggtagcg cagtaacgcg ggttcaaacg cttgtgggat
     4201 atggccgtca ggcgcttcct tcacgcacct acagataacg ttcgcgaatg ggttcaagtt
     4261 gtgggcttga gaattgcgag agccatgctc aagtcaaaac attgaaggag tagaacgaat
     4321 gaaacatttc ataatagcca gcatgcttgc ggcgagcgtt gtgtgtgcgc cattggcgct
     4381 tgcggaggat gcgggtatgt cgactgacac caaccatcac ttcgtgaagg attctgtcat
     4441 tacgacgaaa gtcaaatcga agcttgcggc gaaaaatctg acgaccctga cgcgaatcaa
     4501 agtcgacacc gatgagaacg gcgtcgtttg gctgagcgga cgcgcgccga cgcaagacgc
     4561 ggtcgacatg gcgaccatga tcgcgaagga tacggacggt gtgaagtcgg tgcataacga
     4621 catcgcaatt caaccgtaaa gcgattcggg caggcggacg cctctgaagc gaggcgtccg
     4681 ccgctcacat ctgcgcgatc aatcgatccg caagcagcga caaacacgcg ctcaaggctt
     4741 gcgcgacggc agcgtagtcg tcgtgcgcca gcggcgctct gaacacctca ccccccacga
     4801 catctttgcc tgagtgcggg tcgacaatcc gccaatgcgc ctcgatgctg gcgccctcgc
     4861 cttggcgcac gcttacctgc agaacgtcga cgc
//
LOCUS       DASWZT010000083         3476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_48943, whole genome shotgun sequence.
ACCESSION   DASWZT010000083 DASWZT010000000
VERSION     DASWZT010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48943"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VGE92_02190"
     CDS             <1..967
                     /locus_tag="VGE92_02190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY1312653.1"
                     /translation="SNVVAAAIQEASVRAQIEATRQIIEADEKSLAILRDQFRLGFAM
                     RVDVAAQETSLAMAKATLPPLQKLFEQTRDLLRALVGNLPNQDVPETFELDALQLPPE
                     LPVSLPAKIIEQRPDVRAAEAQLHAANAQVGVAVAAMLPQFSITGSYGGNADQFSWMF
                     RRGGPFWDLIGNATQPLFQGGTLLHRKRAAEQALKQAAAQYQSAVITAYQNVADTLHA
                     SLSDADALAANVDAERAAKVAYELTLRQMQFGYVNTLILLNAETTYNQALLGRVQAQA
                     TRYGDTVALFQALGGGWWNRTTKAAATNATKPGQTASAGQDVATE"
     gene            complement(1002..1715)
                     /locus_tag="VGE92_02195"
     CDS             complement(1002..1715)
                     /locus_tag="VGE92_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1312654.1"
                     /translation="MTEAMHLVLIFEDDEAIQNILRMLFEANGFRVVIADTAARGEQD
                     ARLHRPDVVIVDLGLPDRDGINVISAIRAWSPVPIIVLSARTAEAQRLAAFEMGADDY
                     VMKPFSAPELLARVRAVVRRHVRGDLPMGVLELGDVSIDLGRRVAHRRDGRDLRLTPL
                     EHRILETLARHRDRIVTHAALIKEVWGPVRDNSRALRVYIGSLRRKLEVDPSRPRHIV
                     TELGVGYRLVIEPEPQIEN"
     gene            complement(1802..2191)
                     /locus_tag="VGE92_02200"
     CDS             complement(1802..2191)
                     /locus_tag="VGE92_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1312655.1"
                     /translation="MCLVLIVEDDPGIQDVLRFLAEANDMRVVAADTCDAAIRELEFQ
                     RPDLAIIDLGLPDRDGIHLILNIRRWSHLPIIVLSARTLESQKRAVLQAGADDYVTKP
                     FSAPELLARMRALLHRDLPPAPHPGGG"
     gene            complement(2202..>3476)
                     /locus_tag="VGE92_02205"
     CDS             complement(2202..>3476)
                     /locus_tag="VGE92_02205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1312656.1"
                     /translation="DARYFFTLIVMLVVALVIANLVVSIRRHREIADAREQRTAVLYA
                     MSRELVVATDAQAMAAAAVRHICAVFHSTAVVLIADASGRLSPIAFAEGSAGADLRAC
                     TPHFDPEFAQEVVVSGERRIKEAIYLPLQGSRRVKGVMVVCPEPPSRALPAEQFNLLD
                     AFAAQLAVSLQRARVAEAAEAARISAERVLLRNTLLASISHDLRTPLSAIAGAGSLIA
                     QPEYALNADRRTTLGVLIERKARDMSQLLTNVLDLVQMEFGGRALRSDWHAIDELVAL
                     ALRSSESRLSRWRICLNLPPDLPLILVEPTLIVQILSNLLENAAKYTPPGTTITIGAS
                     VGEEGIFICVADDGPGLPVGDPEQLFEKFQRGRSESNIVGVGLGLAICRAAARLHGGE
                     IHAFNNPQGGARFEISLPVATQADVQPAMESC"
BASE COUNT          702 a         1133 c         1047 g          594 t
ORIGIN      
        1 ctccaatgtc gtcgccgccg ccatccagga agcgtccgtg cgcgcgcaga tcgaggcgac
       61 ccggcagatc atcgaggcgg atgagaaatc cctggccatc ctgcgcgatc aattccgact
      121 aggctttgcc atgcgcgtcg acgtcgcggc acaggaaacc tcgttggcga tggccaaggc
      181 gaccctgcca ccacttcaga agctgttcga gcagacccgc gatctgctgc gtgccttggt
      241 cggcaacctt ccgaaccaag atgtacccga gaccttcgag ctcgatgcgc tgcagctgcc
      301 cccggagctg ccggtgagcc tgcccgccaa gatcatcgaa caacggcccg atgttcgggc
      361 cgcggaggcg cagttgcacg ccgccaacgc gcaggtcggc gtcgcggtcg ccgcgatgct
      421 gccgcaattc tcaatcactg gttcgtacgg cggcaacgcc gaccagttca gctggatgtt
      481 ccgccgcggc ggaccgttct gggacttgat cggcaacgcc acgcagccgc tatttcaagg
      541 cggcaccttg ctgcatagaa agcgcgccgc ggagcaggca ctgaaacagg ccgccgcgca
      601 ataccaaagc gcggtgatca cggcttatca aaatgtcgcc gacaccttgc atgcgtcgct
      661 gtcagatgcc gatgcgctcg ccgccaacgt cgacgcggag agggcggcca aagtggccta
      721 cgaactcacg ctgcggcaaa tgcaattcgg ctacgtcaac acgctgatct tgctcaacgc
      781 cgaaacaact tataaccaag cgctcctcgg ccgcgtgcaa gcccaggcaa cccgctacgg
      841 cgacacggtg gctctgtttc aggccctcgg cggcggctgg tggaatcgca cgactaaggc
      901 tgcggccacg aatgcaacaa agccgggcca aaccgcatcc gcagggcagg atgttgcgac
      961 ggaatgaagc ggcgcctcgg atattcgtca ctcgatgcgc ttcaattttc gatttgcggc
     1021 tccggttcga tgacgagccg gtagccgaca ccaagctcgg taacgatatg tcgcggccgg
     1081 ctcggatcga cctcgagctt cctgcgcaag ctgccgatat agacccgcag cgcccgcgag
     1141 ttgtcgcgaa ccggacccca aacctccttg atgagcgccg cgtgggtcac aatacggtcg
     1201 cggtggcgcg caagcgtctc gagaatccgg tgctcgagcg gagtcagtcg caagtcccgt
     1261 ccgtcccggc gatgagcgac gcgccggccg agatcgatcg acacatcgcc cagttcgagc
     1321 acccccatcg gcaaatcgcc gcgcacgtgg cgccggacga cggcccgcac tcgcgccagg
     1381 agttccggtg cgctgaaagg cttcatgacg tagtcatccg cacccatttc gaacgccgcg
     1441 agccgctgcg cctcggcggt gcgcgccgac agcacaatga tcggcaccgg cgaccacgcg
     1501 cgaatggcgc tgatgacgtt gatgccgtcg cgatcgggga ggccgagatc cacgatcacg
     1561 acatccggcc tgtgcagccg cgcatcctgc tcgccgcgag cggcggtgtc ggcgatgacg
     1621 actcggaagc cgttggcctc aaacagcatg cgcaaaatat tctgaatcgc ctcatcgtct
     1681 tcaaagatca agacgaggtg catggcctcg gtcactctct caccacccta gcgcctgcgg
     1741 cgcgtgcatc ctgccttcgt tccgtagtca ggtcaaacga tgaaatcacg tcgtcgcccg
     1801 attatccgcc ccctgggtgc ggagcaggcg gtaaatcgcg atgcagaagc gcgcgcatcc
     1861 gtgcgagcag ctcaggggcg ctgaatggct tggtcacgta gtcgtcggcg cccgcctgca
     1921 aaaccgctcg cttttgcgac tcgagcgtgc gagccgacag tacgatgatc ggcagatgcg
     1981 accaacgtcg tatgttgaga atcaggtgta tgccgtcccg gtcgggtaga cccagatcga
     2041 ttatggccag gtcgggccgt tggaattcga gctctcgaat cgcggcatcg caagtatctg
     2101 ccgcaacgac ccgcatatcg ttggcctcgg caagaaagcg caaaacatct tgaatccccg
     2161 ggtcgtcctc gacgatgagc acaaggcaca tcgggcaagt ctcagcaaga ctccattgct
     2221 ggttgcacgt cggcttgagt ggccacgggc agcgagattt cgaaccgcgc gccgccttgc
     2281 ggattattga aagcgtgaat ttcgccgccg tgcaggcgag cagccgcgcg gcagatcgcg
     2341 agtcctaagc cgacgccgac gatattgctc tccgatcggc cacgctgaaa tttctcaaac
     2401 aattgttccg gatctcccac ggggaggccg ggaccgtcat ccgccacaca tatgaatatg
     2461 ccctcctccc ccacgctggc gccgatcgta atggtggtgc ccggaggcgt gtatttcgcg
     2521 gcattctcca aaagattgct caaaatctgc acgatgagag ttggctcgac caagatcagg
     2581 ggcaggtccg gcggcagatt caagcagatt cgccatcggg ataggcgcga ctcgcttgac
     2641 cgcagcgcga gtgcgaccaa ctcatcgatg gcatgccagt cagaacgcag agctcggccc
     2701 ccaaactcca tctgcaccaa atccaggacg ttcgtcaaga gctgcgacat gtcgcgggcc
     2761 ttgcgttcga tcaatacccc aagtgtcgtg cggcgatcgg cgttcagcgc atactctggc
     2821 tgtgcaatca ggctgcccgc gccggcaata gccgaaagcg gcgtgcgcag atcgtgagaa
     2881 atcgacgcca gcagcgtgtt gcgaagcaaa acgcgctccg cggatattcg cgccgcctcg
     2941 gcagcctcgg ccacgcgggc tctttgtagc gacacggcca actgagccgc aaaggcgtcc
     3001 aatagattga actgttcggc cggtaatgct cggctcggcg gctccggaca caccaccata
     3061 acacccttga ctcgtcgact gccttgcaag ggcagataga tggcttcctt gatgcgccgc
     3121 tcgccgctga cgacgacttc ctgagcgaat tcagggtcaa agtgcggcgt gcatgcgcgc
     3181 aggtctgcgc cagcggaacc ctcggcgaat gctatcggcg acaagcgccc gctcgcgtcc
     3241 gcgatcagga cgaccgcggt gctgtgaaaa accgcgcaga tatgtcggac cgccgccgcc
     3301 gccatcgctt gcgcgtcggt ggcaaccacg agttcgcggc tcatcgcata cagcacggcg
     3361 gtacgctgct cgcgcgcgtc ggcgatctct cgatggcgcc gaatactcac gacgaggttc
     3421 gcgatcacca aggcgacgac cagcatcacg atcagcgtaa agaaatagcg cgcatc
//
LOCUS       DASWZT010000084         3381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_34509, whole genome shotgun sequence.
ACCESSION   DASWZT010000084 DASWZT010000000
VERSION     DASWZT010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34509"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1439
                     /locus_tag="VGE92_02210"
     CDS             <1..1439
                     /locus_tag="VGE92_02210"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HEY1312657.1"
                     /translation="SELAAALLELIVGHAPALADAWQLLGFALGEEQRIPEAANAFAR
                     AAKLNPREPLTALAHAQASLDAGFSAATLFERAHELAADKFAPINGWAASLAAQGQRA
                     AAESLLAETLVPHPEWLAGHKSLAALRWSSGDSLNFARSYAAACAARPQHLPLRLAWF
                     GALAQAHAWDAALKVVADGERLIGTCAAFSVARAFVAGESGDRALAEEQFSRTAGIRD
                     DVLDIAHLRHCLRTAQLEKAESLALELMKRPSAPMIWPYLSAIWRVRGDARAAWLDGA
                     PPYIRSFDLDYSAAELDGLAATLRRIDSAPAPYVEQSVRGGTQTDTDRQLFFRAEPEI
                     QAVRSKVREAIREYVNALPAYVPGHPLLGIERGRIRFSGSWSVRLQPGGFHVSHTHPK
                     GWISSALYVSSPAPSQLGAPPAGWISLGSPPRTLGVDLPPYVQFEPKPGRLVLFPSTM
                     WHETLPFDDGERLVIAFDVMKPNPSTSA"
     gene            complement(1429..>3381)
                     /locus_tag="VGE92_02215"
     CDS             complement(1429..>3381)
                     /locus_tag="VGE92_02215"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HEY1312658.1"
                     /translation="SLYRPTLQGDSGMLKFCVSAILVGALTCAALSRAHEATDPLQPS
                     GAWSEKAFGDAPLPPMGWNSWNAFHTQIDEEKLMGSARAIVATGLRDLGYRYINIDDG
                     WWQKRRQPDGRMMVRTSIFPSAKVSGPEQTSFRPLLDQLHAMHLKAGIYTDIGRNSCS
                     QGYPEPDSLLPTGSVAEREVGSFDHVDQDMSLYFKEWGFDYIKVDGCGLNAFADDTPA
                     VKRGQFRAFPRYVVSDAVNQTRVAEIRDLYGRVRDSLVRIRPSGDFVFSICNWGTANV
                     RAWGRDFGNLWRTSDDIEPRWTRMLYNFDSVSTRELYSGPGHWNDPDMLFVGHGDFDA
                     HHLTEARTHFALWAIEDAPLIIGYDLRDAPKSLLDIWGAAEVVAVNQDPAANQGILAY
                     TSDDLQIIVKQLSTRDEKAVVIFNRGTESTNATLAATQLKFLPTELVRVRDLWNRRDL
                     PAFTKQMSFTLAPRESVMLRVKGRPELNDGIYLSEMPARIYVAADGIRALQADPQIHR
                     PVPLEGATGGTGPRPALAGWGAPRADSTPYNNELRIRGIVYATGIGALANSRLQVKAD
                     EQFKRFSAQVGIDNSTLERSTKVRFEVYGDGRLLVQSPELGFADTAFSMSTGVADVRT
                     IELVAREIGPGRAATLVDWGVAALTR"
BASE COUNT          618 a         1090 c         1040 g          633 t
ORIGIN      
        1 gcagcgagct cgccgcggcg ttacttgagc tcattgtcgg gcacgcgccc gcgctagccg
       61 acgcctggca gctgctcgga ttcgctctcg gcgaggagca gcgcataccg gaggccgcca
      121 acgcctttgc gcgggcagcg aagctcaatc cgcgcgaacc tctcaccgcg ttagcccatg
      181 cccaggcaag cctcgatgcc ggattttcag cggcgacact gttcgaacgc gcgcatgaac
      241 ttgcggccga caagttcgcg ccgatcaacg gctgggcggc atcgctggcg gcgcaaggtc
      301 agcgggcggc cgcggaatcc ctgttggccg agaccctggt gcctcatccg gaatggttgg
      361 ccggacacaa gagcctcgcc gcgctgcgct ggagttcagg tgattcgctg aacttcgcgc
      421 gtagctacgc ggccgcctgt gctgctcggc cgcaacacct gccgctacgc ctggcttggt
      481 tcggtgcact cgcgcaggca cacgcctggg acgcggcgct caaagtggtc gcggacggag
      541 aacggctcat cggcacctgc gcggcgttca gcgtcgcacg tgccttcgtc gcgggtgaat
      601 ccggcgaccg cgcgctggcg gaggagcagt tctcacgaac ggccggcatt cgggacgatg
      661 ttctggatat tgctcatctg cgtcactgcc tgcgcacggc gcagctggaa aaagccgaga
      721 gccttgcgct cgagttaatg aaacggccgt cggcacccat gatctggccg tacctctcag
      781 cgatctggcg cgtgcgtggc gatgcgcgcg ccgcatggtt ggacggcgca cctccctaca
      841 tccgttcgtt cgacctggat tattcggccg ctgaactcga tgggcttgcg gcgacgctgc
      901 gtcggatcga cagcgccccg gcaccctacg ttgagcagtc ggtgcgcggc ggcacgcaga
      961 ccgatacgga tcgtcagctc ttttttcgcg cggaacctga gatccaggcc gtgcgctcga
     1021 aggtccgcga agcgattcgc gagtatgtga acgcacttcc cgcgtacgtg ccgggtcatc
     1081 ctcttctggg catcgaacga ggacgcatcc gattctccgg tagttggtcc gtgcggttgc
     1141 agcccggggg cttccacgtg agtcacacgc atccgaaggg gtggatcagc tcggcgctct
     1201 atgtttcctc gccggcgccc tcacaattgg gggcgccgcc tgccggctgg atcagtctcg
     1261 gcagcccgcc ccgaacactc ggggtcgatc tgccccccta tgtgcagttc gaacccaagc
     1321 cgggccggct cgtgctcttt ccgtccacca tgtggcacga gacactgcca ttcgacgacg
     1381 gcgagcggct ggtcattgcc tttgacgtga tgaagccgaa cccgtcgact agcgcgtgag
     1441 cgcggccaca ccccaatcca cgagcgtcgc agcgcggccg ggaccgatct cgcgcgcgac
     1501 gagttcgatc gtccttacat cggcaacgcc cgtgctcatt gaaaacgccg tgtcggcaaa
     1561 tcccaactca ggagattgca ccagcagtcg gccgtctccg tacacctcga atcgaacctt
     1621 ggtggaacgc tccaaggtgc tgttatcgat acccacttgc gccgagaagc gcttgaactg
     1681 ctcgtcggcc ttgacctgca gccgggaatt ggcgagtgcg ccgattcccg tcgcatacac
     1741 gatgccgcga attctgagtt cgttgttata cggcgtcgag tcggctcgcg gtgcacccca
     1801 cccggccaag gccggccgcg gaccggtacc gccggtagcg ccttcgagcg gcaccgggcg
     1861 gtgaatctgc ggatccgctt gcagcgcccg gatgccatcg gcagcgacgt agatccgcgc
     1921 aggcatctcg gagagataaa ttccgtcgtt gagctcgggc cggcctttga ctctgagcat
     1981 gacggactcg cggggcgcga gcgtgaacga catttgcttg gtgaacgcag gcaggtcgcg
     2041 cctgttccag agatcgcgta cgcgcacgag ttcagtcggc aaaaatttca gctgcgtggc
     2101 ggcaagggtg gcatttgtcg attctgtgcc gcgattgaaa atgacgactg ccttttcatc
     2161 gcgcgtggac agctgcttca cgatgatctg caagtcgtcg ctcgtgtagg ccaaaatccc
     2221 ctgattagct gccgggtcct gattgaccgc gaccacctcc gccgcacccc agatatccaa
     2281 caacgatttc ggggcatccc gcaggtcgta gccgatgatg agcggcgcat cctcgatcgc
     2341 ccacagcgcg aaatgagtcc ttgcttcggt caagtgatgg gcatcgaagt cgccgtgccc
     2401 cacgaacagc atatccgggt cattccagtg cccgggtccg gaataaagct cgcgggtcga
     2461 gacagaatcg aagttgtaga gcattcgcgt ccaccgcggt tcgatgtcat cgctggtgcg
     2521 ccacagattc ccgaagtccc ggccccacgc gcgcacattg gccgttcccc aattgcagat
     2581 tgaaaagacg aagtcgccgc tcggtcggat gcgcaccaat gaatcgcgca ctcgcccgta
     2641 caggtcgcgg atctcggcga cccgagtttg gttgacggca tcagacacga cgtaccgggg
     2701 aaaggcgcgg aactggccgc gcttcacggc cggtgtgtca tcggcgaacg cattcaagcc
     2761 gcatccatcc accttgatgt agtcgaagcc ccactctttg aaatagagcg acatgtcctg
     2821 atcgacgtga tcgaagctgc cgacctcgcg ctcagcgacg ctccccgtgg gcaaaagaga
     2881 atcgggctcc ggatagccct gcgaacaaga attgcggccg atgtcagtgt aaatgcccgc
     2941 cttcaaatgc atcgcgtgca attggtcaag cagagggcga aaactggtct gctccggccc
     3001 ggatactttg gcgctcggaa aaatgctggt gcgcaccatc atgcggccgt ccggttggcg
     3061 ccgcttctgc caccagccat catcgatgtt gatgtagcgg tatcccaaat cacgcagacc
     3121 cgtggcgacg atggccctcg ccgaacccat cagcttttct tcgtcgatct gcgtgtggaa
     3181 cgcgttccaa ctgttccagc ccatcggggg cagcggcgcg tcgccgaatg ccttctccga
     3241 ccaggcaccg cttggctgca aaggatcggt cgcctcgtgt gcccgagaaa gcgcagcaca
     3301 agtcaaagcg ccgaccaaga tggccgacac gcaaaatttc aacatgcccg aatcgccttg
     3361 caatgttggt cgatataggc t
//
LOCUS       DASWZT010000085         2199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_73225, whole genome shotgun sequence.
ACCESSION   DASWZT010000085 DASWZT010000000
VERSION     DASWZT010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73225"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VGE92_02220"
     CDS             <1..927
                     /locus_tag="VGE92_02220"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY1312659.1"
                     /translation="YPPGSTVKPLYALAAQKYNIITPLQQQFCPGFFTLPGSANKYRD
                     DEKHGFLDMRQAISRSCDVYFYRVAEKLGIDRMHEFMSAFGYGDLTGIDIPGEKAGLY
                     ASPEWKRRVFKRKADQVWFPGETISMGIGQGPITVTPLQQAHFAAEIAERGQIVATPR
                     LVAAVRAAGSNEIKSRPPVLTKPIDIATREQWDVIYDGMEGAVTTGTAHVAGSNAKYK
                     FAGKTGTAQVVTVKQTESTKHVDADVRKREHAWFIAFAPVEDPKVAISVLVENGGFGA
                     SAAAPIARKVLDAYLLGPDPDDHGPAVPRSAP"
     gene            complement(993..1382)
                     /locus_tag="VGE92_02225"
     CDS             complement(993..1382)
                     /locus_tag="VGE92_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEY1312660.1"
                     /translation="MAYVDIVTALAVLQFIVFGIKVGRARGKYGVTAPAVSGNEIFER
                     HFRVQQNTIEQLLIFLPGLHLFSHYYSPLTASILGVVYLIGREIYAASYVKQPSKRSL
                     GYALSFLPAAILLVGGLLGAVMQLARH"
     gene            1478..1990
                     /locus_tag="VGE92_02230"
     CDS             1478..1990
                     /locus_tag="VGE92_02230"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY1312661.1"
                     /translation="MSLPNRTTRTGTRGYAHLVEVQVAASRVWRALTDPDLIRIWSGQ
                     EAEIDPRKGGLYRVGKRNEDSREAHIDIFDVNRRLRLIYLSGSNWPRGASAVVDDFIL
                     DVRKGEGTTSLRLLGSGVPESPAWDKTYVRMRLGWERLLARIKVTVESPPVPKKPPPP
                     KDPPLPGLDY"
BASE COUNT          421 a          686 c          685 g          407 t
ORIGIN      
        1 tacccgccgg gctcgacggt caagccgctg tacgcgctgg ccgcgcagaa atacaacatc
       61 atcaccccgc tgcagcagca gttctgcccg ggcttcttta ccttgccggg cagcgccaac
      121 aagtatcgcg acgacgagaa acacggcttt ctcgacatgc gccaagccat ttcgcgctcc
      181 tgcgacgttt atttctaccg agttgccgag aagctcggta tcgatcgcat gcatgagttc
      241 atgagcgcct tcggctacgg cgatctgacg ggcatcgata ttccgggcga aaaggccggg
      301 ctgtatgcct cgcccgaatg gaaacgacgc gtgttcaagc gcaaggccga tcaggtgtgg
      361 ttccccggcg aaaccatcag catgggcata ggtcaggggc ccattactgt gacgccgctg
      421 cagcaggcgc acttcgccgc ggaaattgcc gaacgcgggc aaatcgtcgc gacgccgcgt
      481 ctggtggccg cggttcgcgc ggcgggctcc aacgagatca agagccgtcc cccggttctg
      541 accaagccga tcgacatcgc gacgcgcgag caatgggacg tgatctacga cggtatggaa
      601 ggcgcggtca ccaccggcac ggcgcatgtg gccggctcca acgcgaaata taaattcgcc
      661 ggcaagaccg gcacggccca ggtggtcacc gtcaaacaaa cggagagcac caagcacgtg
      721 gatgccgacg tacgcaaacg cgaacacgcg tggttcatcg cctttgcgcc ggtggaggat
      781 ccgaaggtgg ccatttcagt cctggtcgaa aacggcggtt tcggcgcgtc ggccgctgcg
      841 cctattgcgc gcaaggtgct cgatgcctat ctgctgggac ccgatccgga cgatcatggc
      901 cccgccgtgc cccgctccgc gccctgatcg atgaaccgcc ggtgtcctcg ccgggcggaa
      961 aacacgcatc cggcgcccca taggggcatt tctcagtgcc gcgccagttg catcaccgcg
     1021 ccgagcaagc cgccgacgag caatatcgcc gccggcagga acgacagggc gtagcccaac
     1081 gagcgcttcg acggctgttt cacgtagctg gcggcgtaaa tttcacggcc gatcaaatag
     1141 acgacgccta gaatggaggc ggtgaggggg ctgtagtaat ggctgaagag gtgcagtccg
     1201 ggcaaaaata tcagcagttg ctcgatggta ttttgctgga cccggaagtg gcgctcgaaa
     1261 atctcgtttc cggagacggc tggcgccgtg accccgtact tgccgcgcgc ccgcccgacc
     1321 ttgattccga atacgatgaa ttgtagaact gcgagcgcag tcacaatgtc tacataagcc
     1381 atggatactc cccccgtgtt agcagattct agcactgcgc cgatgtcggc tttgcgtcaa
     1441 gctaccggca tgacctgtat gaggctcgaa gtccgcaatg agcttaccga atagaaccac
     1501 ccgcaccggg acgcgcggct acgcccactt ggtcgaggtg caggtggctg cgtcgcgcgt
     1561 ttggcgcgcg ctcaccgatc ctgacctcat tcgcatctgg tcgggtcagg aggcggagat
     1621 cgacccgcgc aaaggtggct tgtaccgcgt cggcaaacgc aacgaagaca gccgcgaggc
     1681 gcacatcgat atcttcgatg tgaatcgccg cctgcggctg atttacttga gcggcagcaa
     1741 ctggccgcgc ggcgcgagcg ccgtcgtcga tgacttcatc ctggatgtgc gcaagggcga
     1801 gggcacgacg tcgctgcggt tgctcggctc gggagttccc gagtcccccg cctgggacaa
     1861 gacctacgta cgcatgcgtc tgggatggga gcgcctcttg gcgcgcatca aggttacggt
     1921 agaaagcccg ccggttccca aaaagccgcc gccgccgaaa gacccgccgc tgcccgggct
     1981 tgattattga ttgagcttcg gtcgtccttg atatgctcgc cccgacacat ttgcggagtt
     2041 cagcatgctc gggttcttga tacgcgccag catcgttgcc ctgggcttat ggttggcgac
     2101 ggtttgggtc ggcggcgtct ccatcgacac gccggccacg ttgattcttg cgggcctctt
     2161 gttgggcatc gtcaattccg tggtccggcc catcgccat
//
LOCUS       DASWZT010000086         9473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_63591, whole genome shotgun sequence.
ACCESSION   DASWZT010000086 DASWZT010000000
VERSION     DASWZT010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9473
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63591"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="VGE92_02235"
     CDS             <1..1330
                     /locus_tag="VGE92_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY1312662.1"
                     /translation="WGISRQRYWGCPIPVVHCAACGTVPVPDAQLPVVLPVDLVPDGS
                     GNPLLKNEAFLAVSCPRCGGNARRETDTMDTFVDSSWYFLRFACPDNETAMLDSRVSY
                     WAPVDQYIGGIEHAILHLLYSRFWTRFMRELGAVAFKEPFAHLFTQGMVLNEVFFRKP
                     ASGRVEYFNPADVEVTLDATAQRRIAVLRADGEEVESGGVVTMSKSKNNGVDPQALVD
                     EFGADTARLFTMFAAPPEQTLEWSDEGVQGAYRFIKRLWKAVYEHVTQGVPAPLDKTA
                     LNDGQRALRRQAHQTLAKITDDIGRRRTFNTAIAAAMELLNAVAKHPHSAPQDRSVVH
                     EALEVAVLGLSPIIPHATHALWRALGHETALIDESWTPVDVEALRTAVITLVVQVNGK
                     LRGHITVAVDADEATARAAALADPNVQKFVGTAVVRKVIIVPGKLVNIVV"
     gene            1338..1844
                     /gene="lptE"
                     /locus_tag="VGE92_02240"
     CDS             1338..1844
                     /gene="lptE"
                     /locus_tag="VGE92_02240"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEY1312663.1"
                     /translation="MMSLLRARVLLAAAAWAVAAGMSACGFHLVHPDPLPGILARPYL
                     SLKDPYTDFSREFEHQLKGAGATLQVVRANSTASIDVTQDLVEQRTLSVSALNIPTEY
                     ELTYTVTFAVRGPDKELLAPQTLSLSKDYSFEENVLLAKEHEADILRQQMARDLVSIA
                     MRRLTSLK"
     gene            1858..2337
                     /locus_tag="VGE92_02245"
     CDS             1858..2337
                     /locus_tag="VGE92_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312664.1"
                     /translation="MLLVNAIDRLSLELLLDGYGLQLRLVAPDEVIPGSYWGEREAGL
                     IGAQIFARLDTPLHSVLHEGAHFICMTPERRAGLDTDAGGDDAEEGAVCYLQVVLADL
                     LPNVGRERICRDMDEWGYSFRLGSAATWMAQDADDARDWLMRHGVLDRDGRVTHACR"
     gene            2324..3208
                     /locus_tag="VGE92_02250"
     CDS             2324..3208
                     /locus_tag="VGE92_02250"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY1312665.1"
                     /translation="MPADSPGRVSRRAVLASFSAAFAGGCGRLAFWVANAPASFGAYR
                     RHADIAYGEDAQQRLDVYVPEAAAVQPRPVIVFWHGGRWTFGDKTDYRFVGAALAELG
                     CVAVLPNYRHYPQVKLPGFMHDAAHAALWAATHAADYQADAGRRLFLMGHSAGAHLAA
                     LVTLDPRYFAATGQRAPPIAGVIGLSGPYDFLPLLEADTQDMFGPPAKYPQSQPIEFV
                     RGDAPPMLLVHGLQDDTVWPKNSRNLATALAARGVAVTLKLYPKLVHADTVAALSLPA
                     RGRAPTLADIAAFVAPTS"
     gene            complement(3232..4350)
                     /locus_tag="VGE92_02255"
     CDS             complement(3232..4350)
                     /locus_tag="VGE92_02255"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEY1312666.1"
                     /translation="MGVFEMKDIVIVGAGKIGSMIAELLRGTGDYAVTVVDRSQQQLD
                     RLETAAPIGKIAADITQGDTLRKILAGKFAVLSAAPYHATRLIAEAAKAAGAHYLDLT
                     EDVASTRAVKQLSVGARTAFIPQCGLAPGFITIVASDLASRFDQLQDVRMRVGALPKF
                     PSNALNYNLTWSTDGVINEYCEPCEAIVNGQLRETQPLEELEEFSLDGILYEAFNTSG
                     GLGTLCETLAGKVRNLNYRTIRYPGHAAIMKALLNDLRLRDRRELLKDILENAVPITL
                     QDVVIVFVTVSGMRGGQLMQETYANKIYAAPVGGRVRSAIQITTAGGICAVLDMLSAG
                     KLPQSGLIRQEDIKLPAFLANRFGRCYAMAPALAANAA"
     gene            4465..4881
                     /locus_tag="VGE92_02260"
     CDS             4465..4881
                     /locus_tag="VGE92_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY1312667.1"
                     /translation="MDERLLLALRENGRASTAELARLVGRSRTSVQSRIERLEKQGVI
                     VGYGVLLAPEHSLGAVRAHVMIKVGPKEARAVTAALAAISQVRVLHSVSGDVDLIAVA
                     ATASVAEMDQVIDRIGALDGVERTTSSIILSTKFER"
     gene            complement(4922..5701)
                     /locus_tag="VGE92_02265"
     CDS             complement(4922..5701)
                     /locus_tag="VGE92_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312668.1"
                     /translation="MRIPRMLLALIAFCFSSAALADSPMDGTWALNPNKSNLAGNILT
                     FEDVGGGMMKFVDSSQTYTFKPDGSAFTTPMGVERTFQKGADGGYTSTNKRNGTLLST
                     STWKLSSDANTLTIESKGTKPNGDTFDDVTTFARTSPGMGLIGGWKSTKVKLSSPNSL
                     TIQTDAANGVVLTISAIKATCQAKWDGKDYPANGPTVANGLTLALTKTAPNSFKLVQK
                     VKTKVLVIIRYQVAADGKTMTAKGTNGEGAEPFSEVYDKQG"
     gene            5951..6112
                     /locus_tag="VGE92_02270"
     CDS             5951..6112
                     /locus_tag="VGE92_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312669.1"
                     /translation="MKTMLTMLIAAYLTCGCVSGGNVTHEDSPAATQRDAGPALCRDG
                     TTPPCNDRG"
     gene            complement(6373..7782)
                     /locus_tag="VGE92_02275"
     CDS             complement(6373..7782)
                     /locus_tag="VGE92_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY1312670.1"
                     /translation="MTQPSTPAPSSPHELSRSLKGRHLTMISIGGIIGAGLFVSSSTS
                     IIAGGPASFISYAITGLLILLVMRMLGEMATALPQVRSFTEFARAGLGEGAGFVIGWL
                     YWYFWVLVVPVEAIAGAKILQHWLPVFSPLQIGVGLMTIMTCVNLMSARSYAEFEFWF
                     ASIKVAAILVFIAIAASYAFGWNATHAATFGNLVNYGGFTPNGWIAVLAAVPTVFFAM
                     TGAEITTIAAAESAQPERAVAKLSTTVILRILIFYVVSLFLIVSVTPWTLVRSGESPF
                     TLALNSMHVPWAPQMMSVIILTAVLSCLNSAFYVSSRVLFILADRGDAPKSLVTLNAR
                     RVPVVSVLIGALAGFLGIIAATKAPEVVFNFLVSSSGALIVFVYMTICIAQIALRRRR
                     ERAAQPVPPVSMWLFPYLSYAAIAAMAAVLLAMAFTPRLQRDFYFSCITLVVAILAYV
                     LVQRVRQPRIAASAATWRP"
     gene            complement(7813..>9473)
                     /locus_tag="VGE92_02280"
     CDS             complement(7813..>9473)
                     /locus_tag="VGE92_02280"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1312671.1"
                     /translation="LEPRFNRRTDHYGGSFANRSRFFIELLHALKRAVGDRCAITTRF
                     EVDTLHGPEGVEAREDGVRMLDALRREGVVDAVALKIGDYAEWGEDAGASRFRKSGWM
                     TPFIKQGKAILGPGIPVVGNGRLTSPDDMVGLIRAGVLDIIGAARPSIADPFLPTKIS
                     EGRVEDIRECIGCNICVSKFQQGGQIMCTQNVTIGEEYRRGWHPEKFTKTTQPCSVLI
                     VGGGPAGMECARILGERGYVVHLREADADLGGHWKSVATLPRLSEWGRVISYRQTQLA
                     KLKKTVQVHLGVGRMTADEVINYGADRVVIATGAEWCADGRSANFGPIPGADATLAHV
                     LTPFQVTAGKPVPGKRVLILDGDGHFMGITLAEHMANQGKEVTYVCDAPDVAEYGVFT
                     MESFNNKRMLFEKGVKLYCSHWADSIEPGKVRLSYLYKYGPDLTAPSTGEIPRRNNGG
                     DFDLPVDAVILVTSRQSDDRLWRALKARSADWGRNEIQDMFRTGDCKAPAQLNQALWD
                     AHRLAREFDSPHPAYPLPWIRERQLWGGPTIPKLGDPRACVEAG"
BASE COUNT         1730 a         2887 c         3071 g         1785 t
ORIGIN      
        1 ctggggtatc tcgcggcagc gctattgggg ctgtccgatt ccggtggttc actgcgctgc
       61 ttgcggcacc gtgccggtgc cggacgcgca attgccggtg gtgctgccgg tggatctcgt
      121 gcccgacggc agcggcaatc ctttgctcaa gaacgaagcg tttctcgcgg tttcgtgtcc
      181 gcggtgcggc ggcaacgcgc ggcgcgaaac cgacaccatg gataccttcg tcgactcttc
      241 ctggtacttc ctgcgctttg cctgtccgga caatgagacg gcgatgctcg attcgcgggt
      301 gtcgtattgg gcgcccgtcg atcaatacat cggcggaatc gaacacgcga tcttgcatct
      361 gctgtactcg cgattctgga cacgcttcat gcgcgagttg ggcgcggtgg cgttcaagga
      421 gccattcgcc cacctgttca cgcagggcat ggtgttgaat gaggtgttct tccgcaagcc
      481 cgcctccggc cgcgtcgaat atttcaaccc cgcggatgtc gaggtcaccc tggatgccac
      541 cgcgcagcgg cgcatcgcgg tactgcgtgc cgacggcgag gaggtcgagt ccggcggcgt
      601 cgtcaccatg tcgaagtcca agaacaacgg cgtcgatccg caggcgctgg tcgatgagtt
      661 cggcgccgat accgcgcgct tgttcaccat gttcgcggcg ccgcccgaac agacgctgga
      721 atggtcggac gagggcgtgc aaggtgcgta tcgcttcatc aaacgcttgt ggaaagccgt
      781 atacgagcat gtgacgcaag gcgtgcctgc gccgctcgac aagacagcgt tgaacgacgg
      841 gcagcgcgcg ctacggcgcc aggcgcacca gaccctggcg aaaatcaccg acgatatcgg
      901 ccggcgccgt accttcaata cggcgattgc cgccgcgatg gagctcttga acgcagtggc
      961 gaagcatccg cacagcgcgc cgcaggatcg aagcgtggtg cacgaagctc tggaagtcgc
     1021 ggtgctcggg ctgtcgccga tcattccgca cgcgacccat gcactgtggc gcgcgctcgg
     1081 acacgagacc gcgctgatcg atgaatcctg gacgccggtc gatgtggagg cgctgcgcac
     1141 ggcagtcatc accctggtcg tgcaggtcaa cggcaagctg cgcggccata tcacggtcgc
     1201 cgtcgacgcc gacgaggcga cggcgcgcgc ggctgcgctc gcagatccca atgtgcaaaa
     1261 gttcgtcggc acggcggtgg tgcgcaaggt gatcatcgtt ccgggtaagc tcgtcaacat
     1321 cgtggtgtaa tcgcggcatg atgtccctcc ttcgcgcgcg cgtgttgctt gccgcggccg
     1381 catgggcggt cgcggcggga atgtcggcgt gcggcttcca tctggttcat ccggatccgt
     1441 tgccgggaat cttggcgcgc ccgtatctgt cgctcaagga tccgtacacc gatttctcgc
     1501 gcgagttcga gcatcagttg aagggtgcgg gtgcgacctt gcaggtggta cgcgcgaact
     1561 ccaccgccag cattgatgtg acccaggatc tggtcgagca gcgcaccttg tcggtctcgg
     1621 ccctcaacat tccgaccgaa tacgaactca cctacaccgt gacattcgca gtgcgcggcc
     1681 ccgacaagga attgctggcg ccgcagacgc tcagcctgtc gaaggattac agcttcgagg
     1741 aaaatgtgct gctggcgaag gagcacgagg cggatatctt gcggcagcaa atggcgcgcg
     1801 acctcgtgag cattgcgatg cgccggctga cgagcctgaa gtaaggccgg cacaccgttg
     1861 ttgctggtga acgccatcga ccggctgagc ctcgagctgc tgttggacgg ctatggtctg
     1921 cagttgcgct tggtggcgcc cgatgaagtc atccccgggt cgtactgggg cgagcgcgaa
     1981 gcgggcttga tcggcgcgca aattttcgcg cggctcgaca cgccgctgca ctcggtgctg
     2041 cacgaaggcg cgcacttcat ctgcatgacg ccggaacggc gcgccggttt ggatacggac
     2101 gcgggcggcg acgacgcgga ggaaggcgcc gtgtgctacc tgcaggtggt attggcggat
     2161 ttactgccga acgtgggacg ggagcggata tgtcgcgaca tggatgaatg gggatatagt
     2221 tttcgtttgg gcagtgccgc gacctggatg gcgcaggatg ccgacgatgc gcgcgattgg
     2281 ttgatgcggc acggggtgct cgatcgcgat ggcagagtca cgcatgcctg ccgatagccc
     2341 gggccgcgtc tcgcgacgtg cggtgctcgc gtcattctct gcggccttcg ccggcggttg
     2401 cggccggctc gcgttctggg tggcaaacgc gccggcctct ttcggtgcct accggcgtca
     2461 tgccgacatc gcctacggtg aggatgcgca gcagcgcttg gacgtctacg tgcccgaggc
     2521 ggcggccgtg caaccgcgcc cggtgatcgt gttctggcac ggcggccgtt ggactttcgg
     2581 cgacaagacc gactaccgat tcgtcggcgc ggcgttggcc gaattgggtt gcgtcgcggt
     2641 gttgccgaat tatcggcact acccgcaggt caaactgccg ggattcatgc acgatgccgc
     2701 gcacgccgcg ttgtgggccg cgacgcacgc cgccgattac caggcggatg ccggccgccg
     2761 gcttttcttg atgggccatt cggcgggcgc acatctcgcg gccttggtga cgctcgaccc
     2821 gcgctacttc gctgcaaccg ggcagcgcgc gccgccgatt gctggcgtga tcggactgtc
     2881 ggggccgtac gatttcctgc cgctgctcga agcggatacc caagacatgt tcgggccgcc
     2941 ggcgaagtat ccgcagtcgc agccgatcga attcgtgcgt ggcgacgcgc cgccgatgct
     3001 gttggtgcac ggattacagg acgacacggt gtggcccaag aattcgcgca acctcgcgac
     3061 tgcgcttgcg gcgcgcggcg tcgcggtgac gctcaagctg tacccgaagc tggtgcatgc
     3121 cgataccgtg gccgcgctca gcctgccggc ccgcggccgt gcgccgacgc tcgccgacat
     3181 cgcggctttc gtcgctccga cctcataaag gcgaggtcaa ggagccacag gctaggcggc
     3241 gttggccgcc agcgccggtg ccatcgcgta gcaccggccg aagcgattgg cgagaaacgc
     3301 cggcagcttg atgtcttctt ggcggatgag cccggattgc ggcagcttgc cggcgctcag
     3361 catgtcgagg accgcgcaaa taccgcccgc ggtggtgatc tggatggcgc tgcgcacgcg
     3421 accgcccacg ggtgcggcat agatcttgtt ggcgtaggtc tcctgcatca actgcccacc
     3481 gcgcattccg ctcacggtga cgaatacgat caccacatcc tgcagcgtga tcggtacggc
     3541 gttctccagg atgtccttga gcagttcgcg gcgatcgcgc aggcgcaagt cgttcagcag
     3601 cgccttcatg atcgcggcat ggccgggata gcggatggtg cggtagttga gattacgcac
     3661 tttgccggcg agggtttcgc agagggtccc gagaccgccc gaggtattga acgcttcgta
     3721 caaaatgccg tcgagcgaaa attcttccaa ctcctcgaga ggctgcgtct cacgaagttg
     3781 tccgttgacg atggcttcgc acggttcgca gtattcgttg atgacgccgt cggtgctcca
     3841 ggtcagattg tagttgagcg cattggatgg aaatttcggc aacgcgccga cccgcatgcg
     3901 cacatcctgc agctgatcga agcgtgaggc caggtcgctc gcgacgatgg tgatgaaccc
     3961 cggcgcgagg ccgcactgcg gaatgaacgc ggtgcgcgca ccgacgctca gctgtttcac
     4021 ggctcgggtg ctggcaacgt cctcggtcaa atccaagtag tgggcgccgg ccgctttggc
     4081 tgcttccgcg atgagccggg tggcgtggta gggagcggca ctcagcacgg cgaatttgcc
     4141 cgcgagtatc ttgcgcaggg tgtcgccctg ggtgatgtcg gcggcgattt tgccgatcgg
     4201 agccgcggtt tcaagccggt ccagttgctg ctgcgagcgg tcgaccacgg tgaccgcgta
     4261 atccccggta ccccgcagca actcggcgat catggaaccg attttgcccg cgcctacgat
     4321 aacgatgtct ttcatttcaa aaactcccaa agtattgcgc cattttgggg cacgttccct
     4381 gcgattggta gggttgtttt agacgttttt aaggtatagt cactgcacta cgcagggcaa
     4441 aagcggcgaa atgccataga caccatcgac gaaagactat tgctggcctt gcgggagaac
     4501 gggcgcgcct ctaccgccga attggcgcgg ctcgtcggac ggtcgcgcac cagcgtacaa
     4561 agccgcatcg agcgcctcga gaaacagggc gtcatcgtcg gctacggtgt tctgctggcg
     4621 ccggagcata gcttgggtgc cgtgcgcgcc cacgtgatga tcaaagtggg tcccaaggag
     4681 gcgcgagcgg tcacggccgc gctcgccgcc atttcccagg tgcgggtcct gcattcggtg
     4741 agcggcgatg tcgacctgat cgcggtcgcg gccaccgcct cggtcgccga aatggaccaa
     4801 gtgatcgatc gcatcggcgc actcgacggc gtggaacgca ccacgtcgtc gatcatccta
     4861 tcgaccaaat tcgagcgttg acgccgatct gcgcgcttat acgtcaaggc tcccagggag
     4921 gctagccttg cttgtcgtaa acctcgctaa agggctcggc accctcacca ttggtccctt
     4981 tggccgtcat ggtctttccg tccgccgcga cctggtaacg gatgatgacg agcaccttgg
     5041 tcttcacctt ctgcaccaat ttgaaactgt tgggtgcggt tttggtgagg gcgagcgtaa
     5101 gtccattcgc caccgtcggt ccgttcgccg ggtaatcctt gccgtcccat ttggcctggc
     5161 aggtggcttt gatcgccgaa atcgtcagga ccacgccgtt cgccgcatcg gtctgaatcg
     5221 tgagggagtt cggcgagctc agtttgacct tggtgctctt ccatccgccg atcaagccca
     5281 tgccggggga ggtgcgggcg aaagtggtca catcgtcgaa ggtatcgcca ttcggcttgg
     5341 tgcccttgga ttcgatcgtc agcgtgttcg cgtcggagga gagcttccac gtactggtgc
     5401 tcagcagcgt cccgtttctc ttgttggtgc tggtgtagcc gccgtcggcc cccttctgaa
     5461 acgtgcgttc gacccccatg ggcgtcgtga acgcgctgcc atccggtttg aaggtgtagg
     5521 tctgcgagga atcgacgaac ttcatcattc caccgccgac atcttcgaag gtcaagatgt
     5581 tgccggccaa gttgctctta ttcggattga gcgcccaggt gccgtccatc ggactatcgg
     5641 ccaacgcggc cgaggagaag caaaaggcaa tcagagccag cagcattctc ggaattctca
     5701 tgagggtctc ctggattaat tttctttgcg agagccggtg agccccgatt atcggcccgc
     5761 cccccgggtc gacacaatcc caggaggtcc agatttggag ggccgcgcgc tgcggcaaaa
     5821 gtaggacgat cgcggacaag gtcgtctggg tttgggccct ataccctcgc gaagcacggg
     5881 gcgatcatga tgctctgtgg ctttacgcgg gttttacgcg cgtggcaaag gcgatgcgaa
     5941 ggagagtaac gtgaagacga tgctgacaat gctgattgcc gcttacttga cgtgcggctg
     6001 cgtaagcggg ggcaacgtga cgcacgagga tagtcccgcg gccacccagc gcgacgcagg
     6061 tccggcgctg tgccgcgacg gcaccacacc gccgtgtaac gaccgcggct gagactgcag
     6121 tcagaagttt tcagcgggga ataaaaaacg gcgtcggcct tttgaggtac cgacgccgcc
     6181 gggatatcgc accgacgggg gagttggctg gcttgccggg gcgagtactc caaaccttaa
     6241 gcctcgaact ctgttcctgc agatgttcct tgaggcacaa ctcatcctgc caaaataaag
     6301 aacctggggg gcggaccctt tatatccgat tgtgtagtta ttttcgtttg acaatctgga
     6361 aaagatcgat tttcacggac gccaggtcgc ggccgaggct gcgatccgcg gctgacgcac
     6421 gcgctgtacg agcacatagg cgagaatcgc cactaccagc gtgatgcagc tgaagtagaa
     6481 atccctctgc aatcgcggcg taaaagccat cgccagcaag accgccgcca tagcggcgat
     6541 ggccgcataa ctcaagtacg ggaacagcca catgctgacg ggcggcacgg gctgtgccgc
     6601 gcgttcgcga cgccggcgca gcgcaatttg cgcaatgcaa atcgtcatgt acacgaaaac
     6661 gatgagagcg ccggaagagc tgaccaaaaa attgaatacc acttcaggtg ccttggtggc
     6721 ggcgataatt cccaaaaatc cggcgagcgc gccgatgagc accgacacca ccggcacgcg
     6781 acgcgcattc aacgtcacca gagattttgg cgcatcgccg cgatcggcca atatgaagag
     6841 cacgcgcgaa ctcacataga acgcagaatt caaacaggac agcaccgcag tgagaatgat
     6901 gacgctcatc atctgcggcg cccacggtac gtgcatggaa ttgagcgcca gggtgaaggg
     6961 cgattccccg gagcgcacca gcgtccaagg cgttaccgaa acgatcaaga acagcgatac
     7021 gacgtaaaaa atcaggatgc gcaagatcac ggtggtgctc agtttggcca cggcccgctc
     7081 gggctgcgcc gactctgcgg cggcaatggt ggtgatttcc gcgcccgtca tggcgaagaa
     7141 caccgtcggg acggcggcca acacggcaat ccacccattg ggggtgaagc cgccgtagtt
     7201 gacgagattg ccgaacgtcg cagcgtgcgt ggcgttccaa ccgaacgcat acgaggcggc
     7261 gatggcgatg aacaccagaa tggcggcgac cttgatcgag gcaaaccaga attcgaattc
     7321 ggcgtacgaa cgcgccgaca tcagattgac gcaggtcatg atggtcatga gtccaacgcc
     7381 gatctgcagc ggggagaaaa ccggcagcca gtgctgcaga atcttggcgc ccgcaatggc
     7441 ctcgaccggc accaccagca cccagaagta ccaatacagc cagccgatca cgaatccagc
     7501 cccctcgccg agtccggcgc gcgcaaattc ggtgaacgag cgcacctgcg gcagcgccgt
     7561 ggccatctct cccagcatgc gcatcaccag gagaatcagc agtccggtga tggcatagct
     7621 gatgaaactc gccggcccgc cggcgatgat cgaggtgctg ctgctcacga acaggcccgc
     7681 accgatgatt ccgccgatgg aaatcatggt caggtggcgg cccttgagcg agcggctcag
     7741 ctcgtggggg gaagagggcg cgggcgtgct cggttgagtc atcggtcatc cctaggtgaa
     7801 ttgggctgca tcttaccctg cttcgacgca ggcccgcggg tcgccgagct tcgggatggt
     7861 cgggccgccc cacagttgcc gctcgcggat ccagggcagc ggataggccg ggtgcggact
     7921 gtcgaactct cgtgccagcc ggtgcgcatc ccacagcgcc tgattgagct gcgcgggcgc
     7981 cttgcagtca ccggtgcgga acatgtcctg aatctcgttc cgtccccagt cggcgctgcg
     8041 agccttcagc gcgcgccata gccggtcatc cgattggcgc gaggtcacga ggatgacggc
     8101 atccaccggc aagtcgaaat cgccgccatt attcctgcgc gggatctccc cggtgctggg
     8161 cgccgtcagg tcgggaccgt acttatacag ataactcaag cggaccttgc cgggttcgat
     8221 cgaatcggcc cagtggctgc agtagagctt gacgcccttt tcgaacagca tgcgtttgtt
     8281 gttgaaggac tccatggtga agacgccgta ctcggcgaca tcgggagcgt cgcacacata
     8341 ggtcacctcc ttgccttgat tggccatgtg ctcggcgagc gtgatgccca tgaaatggcc
     8401 atcgccatcg agaatcagga cgcgctttcc cggcacgggt ttgcctgcgg tgacttgaaa
     8461 gggggtcaac acgtgcgcca gggtcgcatc ggcgccgggg atcggcccga aattggcgct
     8521 ccggccgtcg gcgcaccact cggcgccggt ggcgatcacc acgcggtcgg cgccgtagtt
     8581 gatcacctca tcggccgtca tgcggcccac gccaagatgg acttggacgg tcttcttcag
     8641 cttggccagt tgcgtctgcc ggtagctgat cacgcggccc cactcgctca accgcggcaa
     8701 tgtcgccacc gacttccagt gaccgccgag atcggcgtcg gcttcccgaa gatgcacgac
     8761 gtagccgcgc tccccgagaa tacgcgcgca ttccatgccc gcagggccgc cgccgacgat
     8821 gagcacggag cagggttgcg tggttttcgt gaatttctcc ggatgccagc cgcgccggta
     8881 ttcctcgccg atggtcacat tctgcgtgca catgatttgg ccgccctgct ggaacttgga
     8941 cacgcagatg ttgcagccga tgcactcgcg gatgtcctcg acccggcctt ccgagatctt
     9001 ggtcggcagg aatggatcgg cgatggaggg ccgcgcggcg ccgatgatgt cgagcacccc
     9061 ggcgcgtatc aagccgacca tgtcatcggg gctggtcaag cgtccgttgc cgaccaccgg
     9121 aatgccgggc ccaagaatgg ccttgccctg cttgatgaac ggcgtcatcc aaccggactt
     9181 gcgaaagcgc gaggcgcccg catcttcgcc ccactcggca taatcgccga ttttcaatgc
     9241 caccgcgtcg accacgcctt cgcggcgcag agcatcgagc atgcgcaccc catcttcgcg
     9301 ggcttcgacg ccttcgggcc catgcagggt gtcgacctcg aagcgagtgg tgatggcgca
     9361 gcgatcgccc accgcgcgtt tcaaagcgtg cagcagctcg atgaaaaacc tcgagcggtt
     9421 ggcgaagctg ccgccgtaat ggtcggtgcg gcggttgaag cggggttcga gaa
//
LOCUS       DASWZT010000087         3436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_10392, whole genome shotgun sequence.
ACCESSION   DASWZT010000087 DASWZT010000000
VERSION     DASWZT010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10392"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..886)
                     /locus_tag="VGE92_02285"
     CDS             complement(158..886)
                     /locus_tag="VGE92_02285"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY1312672.1"
                     /translation="MANSQRTTNSITRRAIGSLLALLCSGAFIAAALPAHQVREDIEW
                     LDVWLPHTNDHDLPHVLLIGDSITRGYGPEVEKALAGRVYVGRLATSKSLGDPALLSE
                     VELILRNNRVDVIHFSNGLHGSKYTEAEYVAAIPALLAAFKRTAPKARLIWASSTDVV
                     GARRPDDLTPSRIAERNRLAATVVTKQGIPVDDLFTVVNNHADYHLADGVHFTAQGYR
                     ILAGHVAASVSSVLDGQNPQPSHP"
     gene            952..1629
                     /locus_tag="VGE92_02290"
     CDS             952..1629
                     /locus_tag="VGE92_02290"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEY1312673.1"
                     /translation="MKNLLPYAAVLAMLCGSLANAASQWQSLLQDDAAPAWRGWSAPG
                     LPAGWHVSGGLLSKEGSVDDLVTTQTYADFELELEWKIGKGGNSGIFYRGTREYDHIY
                     WSGPEYQLLDDANASDGKNRLTAAAAAYALYAAPAGVVKPFDEWNTTRLLVRGTHVEH
                     WLNGRKVVDYDLQSPDWKAKVAASKFSAYPHYGLATRGLIGLQGDHTGALSIRNIRIR
                     ELAPNSP"
     gene            1709..2812
                     /locus_tag="VGE92_02295"
     CDS             1709..2812
                     /locus_tag="VGE92_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY1312674.1"
                     /translation="MSLFGHPTDLNRQFIARPDAAKEDILYKWPDMTIRKLTQVTVQP
                     DETALFFKEGKLAGTLPQGRSTMDGALIPFLGDIVDWASGGNMYRAELYFVGTREFVS
                     LPFGGPIDNIEDPETGLAVGLRVFGEYALMVSDPTAMILKLVGTRQASNDLITDWARE
                     QILKALRTSVVKKLTAEKWPVLGLAARTAEIEAALMADVQEPLAPYGLKITRLGNVTI
                     SLKPEDEQTLKGLRKDTAYSRLAGGFREYGVGAALKGIGEGAAAGGGGGTAALGVGMG
                     LGGIVAGLGVGPAPGTAPYPGGGPAPGPTPGSAGSAGGNACANCHAPNAPGAKFCSSC
                     GTAMAPKGVHCTQCGTAAAPGAKFCANCGGALT"
     gene            2809..>3436
                     /locus_tag="VGE92_02300"
     CDS             2809..>3436
                     /locus_tag="VGE92_02300"
                     /inference="COORDINATES: protein motif:HMM:NF016190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEY1312675.1"
                     /translation="MNRPIGSRVTAGAAYTLGLAPLIFAVAAQARVGAGNHAAASHVA
                     SGGGVSFGSGGSLAGVILLIVLIFGIRYIMRRAGIGGSAGQSMSAAPPAAQTALSVLG
                     SALGASSLARVASGGLDSVAGGSSMRRALPPAIDAIRALDPGFELESFLQRAEMTFFL
                     VKRGIQKNDAAAIRPYLSDSVFDAVSRNIAQSKSQHRHTLLESLNIRGL"
BASE COUNT          654 a         1021 c         1133 g          628 t
ORIGIN      
        1 gacccccggc aagccggtaa cactcgtaga acccatcgag caggccgagg ggcgtggtca
       61 cgagcagcag cggcttggcg attctcatgg catgtattgt cgcagagcgg cgcttgcata
      121 acagatacag aagccgatga aaaaaagcag tacagagcta ggggtgcgaa ggctgcgggt
      181 tttgtccgtc gagaacgctg cttacactcg cagcaacatg acctgccaga atgcgatagc
      241 cctgggcagt gaagtgcacg ccatcggcga ggtgataatc ggcgtggttg ttcaccacgg
      301 tgaatagatc gtcgacggga atgccctgct tcgtgaccac ggttgcggcc aggcgattgc
      361 gctcggcgat gcgcgacggc gtcagatcgt caggccggcg agccccaacg acatccgtgg
      421 agctcgccca gatgagccgt gctttcgggg ccgtccgctt gaatgccgcc agaagtgcgg
      481 gtatggccgc gacgtattcc gcctcggtgt acttggagcc atgcaatcca ttgctgaaat
      541 ggatcacgtc gacgcgattg ttccgcaaga tgagctcgac ctcgctcagc aacgcgggat
      601 ctcccagcga cttcgaggtc gccagtcgtc cgacatagac tcgcccggcc agcgccttct
      661 cgacttcggg accgtatccc cgggtgatcg aatcgccgat cagtaaaacg tgcggcaggt
      721 cgtgatcgtt cgtgtgcggc agccacacgt cgagccactc gatgtcctcg cgtacctggt
      781 gggcgggcaa tgccgcggcg atgaatgcac cgctgcacaa caaggccagc agcgagccaa
      841 ttgcccggcg cgtgatagaa tttgtcgtgc gctgcgagtt agccatggaa tctgaaagat
      901 aacgttttgc gcaaagctct gctattgccg gctggatgct tgaggtgctc aatgaagaat
      961 ttactcccct acgcggcggt gctcgcgatg ctgtgcggct cgctcgccaa tgccgcttca
     1021 caatggcaat cgctgttgca ggatgatgct gcgccggctt ggcggggctg gtcagcgccc
     1081 ggattgcccg ccggttggca tgtttcgggc ggactactct ccaaggaagg ctcggtcgac
     1141 gatctcgtga cgacgcaaac ctacgcggat ttcgagctcg aactcgagtg gaaaattggc
     1201 aagggcggca acagcggcat cttttatcgc ggcacgcgcg agtacgatca catctattgg
     1261 agcggcccgg aatatcagct gctggatgat gcgaacgcgt ccgacggtaa gaaccgcctg
     1321 accgcggccg ccgccgcgta cgcgctctac gccgcgccgg ctggtgtcgt gaagcccttc
     1381 gatgaatgga atacgactcg gctcctggtc cggggcaccc acgtcgagca ctggctcaac
     1441 ggccgcaagg tcgtcgacta tgacctgcag agccccgact ggaaggccaa ggtggcggcg
     1501 agcaaattct cggcctatcc gcattacggc ttggcgacga gaggtctgat cggcctgcaa
     1561 ggcgatcaca cgggagcgtt gtcgattcgc aatatccgaa tccgcgagct ggctccgaac
     1621 agcccgtagc cgaaaaatgg cgggaagctg gtagattagg gatgatccag ccgaataccc
     1681 cgcagtcaag tcaccaggag agtgaatcgt gagcttattt ggtcacccca ccgatcttaa
     1741 tcgacagttc attgcacggc ccgacgccgc aaaagaagac attctctaca agtggccgga
     1801 catgaccatt cgcaagctga ctcaggtcac ggtgcagccg gatgagaccg cgctgttttt
     1861 caaggagggc aagttggcag gtactctccc gcaggggaga tcgacgatgg acggcgccct
     1921 gattcccttt ttgggcgaca tcgtggattg ggcaagcggc ggcaatatgt accgcgccga
     1981 gctttacttc gtcggcacgc gcgaattcgt cagtctgccg ttcggcggac ccatcgacaa
     2041 tatcgaggac ccggagaccg gactcgcggt gggtttgagg gtgttcggcg agtacgcgct
     2101 gatggtcagc gacccgaccg cgatgatctt gaaactagta ggcacccgcc aggcatccaa
     2161 cgacttgatt accgactggg cgcgcgaaca gattctcaag gcgctgcgca ccagcgtggt
     2221 gaagaaactg acggcggaga aatggccggt gttgggtctc gccgcccgga cggcggagat
     2281 cgaagcggcg ttgatggccg acgttcaaga gccgctggcg ccctatggcc tcaagatcac
     2341 tcggctgggt aacgtgacca tcagcttgaa gcccgaagat gagcagacat tgaaaggcct
     2401 gcgcaaggac acagcctatt cgcggctggc cggcggcttt cgcgagtacg gggtcggcgc
     2461 tgcgctcaag ggaatcggtg aaggcgcagc ggcgggcggt ggcggcggca cagcggcgct
     2521 cggcgtcggc atgggtcttg ggggaatcgt cgccggtctt ggagtcggcc ctgcgcccgg
     2581 aacggcgccc tatcctggcg gaggtcccgc tcccgggcct acgccgggga gcgcaggcag
     2641 cgccggcggc aatgcgtgcg cgaattgtca cgcgccgaac gcgccagggg cgaaattctg
     2701 ttcgagctgc ggcaccgcga tggcgcccaa gggcgtgcac tgcacccagt gcggcactgc
     2761 cgcggcaccg ggcgcaaaat tctgtgcgaa ttgcggtggc gcgctgacgt gaatcgcccg
     2821 atcgggtccc gcgtgacggc aggcgctgcg tacacgctgg gattggcgcc gttgatcttc
     2881 gccgtggccg cacaggcgcg cgtcggcgcc ggcaatcatg cggcggcctc gcatgttgcc
     2941 agcggcggcg gagtgtcgtt cggcagcggc ggttcgctgg ccggcgtcat cttgctcatc
     3001 gtgctgatct tcggtatccg ctacatcatg cgccgtgccg gtattggcgg ttccgccgga
     3061 cagtcaatga gtgctgcacc accggctgcg caaaccgcgc tgtcggtgct gggatctgct
     3121 ctcggcgcct caagcctcgc gcgagtcgcg agcggcggcc tcgattcggt cgccggcggc
     3181 tcgagcatgc gacgggcctt gccgcccgca atagacgcca tacgagcgct cgatccgggt
     3241 ttcgaattgg aatccttcct gcagagagcg gagatgacgt tcttcctggt caagcgtggc
     3301 attcagaaaa atgatgccgc ggcgattcgg ccctatctga gcgacagtgt gttcgacgcg
     3361 gtgtcgcgaa acatcgcgca atccaagtca cagcaccggc acaccctgtt ggagagcttg
     3421 aacatccgcg gcctcg
//
LOCUS       DASWZT010000088         2250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_44238, whole genome shotgun sequence.
ACCESSION   DASWZT010000088 DASWZT010000000
VERSION     DASWZT010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44238"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..922
                     /gene="phoU"
                     /locus_tag="VGE92_02305"
     CDS             185..922
                     /gene="phoU"
                     /locus_tag="VGE92_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY1312676.1"
                     /translation="METADLTHHISRRFNEDLEKVRNQVLQMGGFVEQQLHKAITALV
                     EGDSRLGESVATDDYKVNGMEVSIDEECSRILAIRAPAAGDLRVIVAIIKTITDLERI
                     GDEGEKIGYIASRLATMERPADKYREVKHLGRQVAQMVHDALDAFARMDSQAALKTAR
                     EDKMVDEEYEAIQRQCMTFMMEDPRTIRRALEIMWIVRALERIGDHAKNICEYVIYMV
                     HGKDIRHTSLEDVARQIDAAHAIARGG"
     gene            922..1422
                     /locus_tag="VGE92_02310"
     CDS             922..1422
                     /locus_tag="VGE92_02310"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEY1312677.1"
                     /translation="MSRRQGNLLGFAACAALLGYAYYAQVFLHLEPCPLCIFQRVGVF
                     VLGLLFLIAAAQDPVGWGRRVYAALLVSAALATVAVAARHLYIQSMPAGSIPACGASL
                     DFMLKVFSLSEVLVKVLTGSGECAKVTWTFLGLAMPGWVLIAGLGLGIYGLWINLRHQ
                     PPVLRF"
     gene            1422..>2250
                     /gene="dapE"
                     /locus_tag="VGE92_02315"
     CDS             1422..>2250
                     /gene="dapE"
                     /locus_tag="VGE92_02315"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF009557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEY1312678.1"
                     /translation="MPNAPPKSDTLRLTEELIARASVSPDDAGCQQLMINRLEASGFT
                     VDRMRFGPVDNFWAKRGHGAPVFCFAGHTDVVPPGPLDDWNGDPFVPVIKDGLLYGRG
                     AADMKSGLAAMLTSCEEFVTLYPRHRGTMAFLITSDEEGPSVDGTRRVVEVLRERGER
                     IDWCLVGEPSSETALGDTLKIGRRGSLSGRLTVHGIQGHIAYPQLADNPVHAAAPALA
                     ELAARTWDGGNQHFQPTTFQISNISAGTGAPNVIPGELKARFNLRFSTEQSVDGLKRV
                     "
BASE COUNT          421 a          681 c          725 g          423 t
ORIGIN      
        1 ggcagcacgt tcacctgtta tttcccggcg cagcgaattt ggcagcaaac ggttcgcgcc
       61 gcggcgaacg cttgatgtca catttagggc ggctccatgt tgacgtatgc cgcaatcgga
      121 tgtaaacgaa cacccgagta acagacgcgg accatcgtcg cgtgacgcag aaaggtaaat
      181 tcttatggaa accgccgacc tcacccacca tatttcccgc cgcttcaacg aagacctgga
      241 aaaggttcgc aatcaggtgc tgcagatggg cggattcgtg gaacagcagc tccataaggc
      301 aataaccgct ttggtcgagg gcgacagccg cttgggcgag tcggtggcaa ccgacgacta
      361 caaggtcaac ggcatggaag tgtcgatcga cgaggaatgc agccgtatcc tggcgatccg
      421 cgcgcccgcg gccggcgatt tgcgggtgat cgtcgcgatc atcaagacca ttaccgacct
      481 cgagcgcatc ggcgacgagg gcgagaaaat tggctacatc gcttcgcggc tcgccaccat
      541 ggagcgcccc gccgataagt accgcgaggt caaacatctc ggcagacagg tcgcacaaat
      601 ggtgcacgac gcgctcgacg cgttcgcgcg catggactcg caggctgcgc tcaagacggc
      661 ccgcgaggac aaaatggtcg acgaggagta tgaggcgatc caacgccagt gcatgacgtt
      721 catgatggag gatccgcgca ccattcggcg cgcgctcgag atcatgtgga tcgtgcgcgc
      781 actggagcgc atcggcgatc acgccaagaa tatctgtgaa tacgtgatct acatggttca
      841 cggcaaggac attcgccaca cttcgctcga ggatgtggcc aggcagatcg acgccgcgca
      901 cgccatcgcg cgcggcggct gatgagcagg cgccagggga atttactcgg gttcgcggcc
      961 tgcgcggctc tgctgggcta tgcctattac gcacaggtgt tcctgcatct ggagccgtgc
     1021 ccgctgtgca tttttcaacg cgtcggcgta ttcgtgctcg ggttgctgtt cttgatcgcg
     1081 gcggcacagg acccggtcgg ctggggacga cgcgtgtatg cagcgctgct cgtgtcggcg
     1141 gcgcttgcca cggtcgccgt ggcggcaagg cacctgtaca tccaaagcat gccggcgggt
     1201 agcatccccg cctgcggagc atccctcgat ttcatgctca aggtgttttc cttgagcgaa
     1261 gtgctggtca aggtgctgac cggctccgga gaatgcgcca aggtcacatg gacgtttctc
     1321 ggtctggcga tgccggggtg ggtgctcatt gccggcctcg gtctcggcat ctacggccta
     1381 tggatcaatc tgaggcatca gcccccggtt ttgcgcttct aatgccgaac gcgccgccga
     1441 agtcagacac cttgcggttg acggaggagc tgattgcgcg tgcctcggtc agccccgacg
     1501 atgctggttg ccagcagctg atgatcaatc ggctcgaggc cagcggtttt accgtcgacc
     1561 ggatgcgctt cggtccggtc gataacttct gggcaaagcg cggccacggc gcgccggtgt
     1621 tctgtttcgc cgggcacacc gatgtggtgc cgccgggacc gctcgacgat tggaatggcg
     1681 atcccttcgt tccagtcatc aaggatgggc tcttgtacgg gcgcggcgcg gccgatatga
     1741 aaagcggcct ggccgccatg ctgacgtcct gcgaggaatt cgtgactctc tatccgcgcc
     1801 atcgcggcac catggcgttc ttgatcacga gcgatgagga agggccgtcg gtcgacggta
     1861 cgcgtcgcgt ggtcgaggtg ctgcgcgaac ggggggagcg catcgattgg tgcctggtcg
     1921 gcgagccctc gagcgaaacc gcgctcggcg acaccttgaa gatcggccgc cgcggcagcc
     1981 tcagcggccg tctcacggtt catggcatcc agggccatat cgcctacccg cagctcgccg
     2041 acaacccggt gcatgcagcg gcgccggcgc tcgccgagct tgcggcgcgc acgtgggatg
     2101 gcggcaacca gcattttcag ccgacgacgt tccagatatc gaatatttcg gccgggacag
     2161 gcgcccccaa cgtcattccc ggtgagctca aggcgcgctt caatttgcgc ttttcgaccg
     2221 aacaaagcgt cgacggactg aaacgcgtcg
//
LOCUS       DASWZT010000089         4235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_29699, whole genome shotgun sequence.
ACCESSION   DASWZT010000089 DASWZT010000000
VERSION     DASWZT010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29699"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..1337
                     /locus_tag="VGE92_02320"
     CDS             24..1337
                     /locus_tag="VGE92_02320"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEY1312679.1"
                     /translation="MSTFLGSIAALIWFVALAACSSKAGDPARQYGPNPYLPKPHEYL
                     MPPMSVPKAINWKAGELPTVSAGLKVEALATGLEHPRTLYTLPNGDILVVESNSPGTE
                     PFRPKDFIAGKVKARAGAAAKGGNRITLLREADGKPELRTVFIDHLYSPYGIVLVGDT
                     LYVADTDAILAFNYVPGETRITSPGVKLADLPAGPIDHHWTKSMTASADGSKLYVGVG
                     SNSNITENGMGVEEGRAAIYEVDRLTGAKRIFASGTRNPTSVAFEPQTHQLFAVVNER
                     DEIGPDLVPDYLTSIRQGGFYGWPYSYWGSHVDARVHPQRPDLVAQAIKPDYGLSSHV
                     APLSLLFSEGEHLSPAFANGAFISEHGSWDRSPLNGYQVIFVSFVNGRPVGMPMPVVT
                     GFLGKDDETVHGRPVGLALDRVGALLVADDVGNTVWRVSATQPGT"
     gene            complement(1449..1525)
                     /locus_tag="VGE92_02325"
     tRNA            complement(1449..1525)
                     /locus_tag="VGE92_02325"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1489..1491),aa:Arg,seq:acg)
     gene            complement(1602..1692)
                     /locus_tag="VGE92_02330"
     tRNA            complement(1602..1692)
                     /locus_tag="VGE92_02330"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1656..1658),aa:Ser,seq:gct)
     gene            complement(1781..1993)
                     /gene="csrA"
                     /locus_tag="VGE92_02335"
     CDS             complement(1781..1993)
                     /gene="csrA"
                     /locus_tag="VGE92_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HEY1312680.1"
                     /translation="MLILTRRVGETVMIGNDVTVTVLGVKGNQVRIGVNAPKDVAVHR
                     EEIYERIKREEEHDSRTGTPVAKIVD"
     gene            complement(2169..>4235)
                     /gene="alaS"
                     /locus_tag="VGE92_02340"
     CDS             complement(2169..>4235)
                     /gene="alaS"
                     /locus_tag="VGE92_02340"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEY1312681.1"
                     /translation="GGPPGTPDEDGDRFVEIWNLVFMQYDRSSDGVLVPLPKPSVDTG
                     MGLERVAAVMQGVHSNYDIDLFKSLIRSAAEVTGTHDLESSSLRVIADHIRACTFLIV
                     DGVVPSNEGRGYVLRRIIRRAIRHGYKLGQTQPFFHKLVASLVREMGPYYGELISGEA
                     RATQVLAQEESRFAETLTTGMALLDAAAAKLTSKVIPGETVFRLYDTYGFPLDLTADV
                     ARERGLSIDQAGFDAAMEAQRGRARAASKFGTDLRDSVKLAGKTDFLGYDRLADRGVV
                     TSLIFDGAVVDTLGPGQEGQVVLDHTPFYAESGGQIGDAGVLVRLGQDRVATRFIVRD
                     TQKIGASFAHVGVLDTGILRVGDAVEAQVNGERRTSIALNHSATHLLHAALRKVLGTH
                     VEQKGSLVAAERLRFDFSHTQPLTAEELQRVEELVNSTIRSNAPVETRVMALDDAVAA
                     GAMSLFGEKYESDVRVLSIGDFSLELCGGTHVKRAGDIGLFKILSESGVAAGVRRVEA
                     ITGKTAYEWVVHTEQVLRDVAGMLRGSRDDVDEKVRELVERSRKLEKEVQQLKSKLAT
                     GQGGDLTSQAKNVDGIQVLAAQIEGADAKSLRDAVDQLKSKLGSSVVVLATVQDGKVV
                     LVAGVSADLLTRMKAGDIAGAVAAQVGGRGGGRADFAQAGGTQPENLGRALAGVESLV
                     RSRLSN"
BASE COUNT          838 a         1393 c         1199 g          805 t
ORIGIN      
        1 agccgcgcaa gcggagagtg cccatgagta catttcttgg ctcgatcgca gcgctgatct
       61 ggttcgtcgc cctcgcggcg tgcagctcaa aggccggcga cccggcccgg caatatggcc
      121 ccaaccctta cttgccgaaa ccgcacgagt acctgatgcc gcctatgagc gtaccgaagg
      181 cgatcaattg gaaagccggt gagctgccca cggtttctgc aggtctcaaa gtcgaggcgc
      241 tcgcgaccgg gctcgagcac ccgcgcactc tctatactct gcccaacggc gacatcttgg
      301 tggtcgagag caacagcccc ggcaccgagc cctttcggcc caaggacttc atcgccggaa
      361 aggtgaaggc gcgggccggc gccgccgcca agggcggcaa ccgaatcact ctgctacgcg
      421 aggccgacgg taagcccgag ctcaggacgg ttttcatcga ccatctctac tcgccctatg
      481 gaattgtgct ggtgggcgac acgctgtacg tcgccgatac cgacgcaatc ctcgccttca
      541 actatgtgcc gggggagacg cgcatcactt cgcctggcgt gaagctcgcc gatcttccgg
      601 cagggccgat cgaccatcac tggaccaagt cgatgaccgc gagcgcggac ggttcgaaac
      661 tctacgtcgg cgtgggttca aacagcaaca tcaccgaaaa cggcatgggc gtcgaggagg
      721 gccgtgccgc catttacgag gtcgaccgct tgaccggcgc caagcgcatc ttcgcttccg
      781 gcacgcgcaa cccgaccagc gtggcgttcg aaccgcagac gcatcagttg ttcgctgtcg
      841 tcaacgagcg cgacgaaatc ggtcccgatc tcgtccccga ttatttgacc tcgatccggc
      901 aaggcggctt ctacggctgg ccctacagct attggggaag tcatgttgac gcgcgagtcc
      961 accctcagcg gccggacctc gtcgctcaag cgatcaagcc cgactatggc ctgagttccc
     1021 acgtcgcccc gctcagcctc ctgttcagcg aaggcgagca cctgtcgcca gccttcgcta
     1081 acggcgcctt catcagtgag cacggcagtt gggaccgcag ccccttgaac ggctatcagg
     1141 tgattttcgt atctttcgtg aacggccgcc cggtcggcat gccgatgccg gtggtcaccg
     1201 gctttcttgg caaggacgac gagacggttc acggacggcc cgtgggcctc gcgctggacc
     1261 gcgtcggggc actgctcgtc gcagacgatg ttggaaacac ggtatggcgc gtgagtgcaa
     1321 cgcagccggg aacctaaacc cagcctcgac agcgatcttc ggggcgccat tgacgtcgcg
     1381 agcaaatcgg acgaaggcac gagatcggca ctgcgacgtt ccaagtgaca gttccggcgt
     1441 atgaacaatg gtgcgcccgg agagattcga actcccggcc tcctggttcg tagccaggcg
     1501 ctctatccaa ctgagctacg ggcgctaaag gggcgcgaat attaccacat caatccgtta
     1561 tgaccatttg gccgccttgg ggtgggcgac ctcgattaaa ttggcggaga gggagggatt
     1621 cgaaccctcg atgggctttt gacccatacg cccttagcag gggcgcgcct tcgaccactc
     1681 ggccacctct ccgggcagga cctaagccca ttcgcggagc gcgggatgat acccgtccga
     1741 agcagtctag gtaaagctaa agggcgtgga cccttgaacg tcaatcgaca attttggcga
     1801 ccggggtacc ggttcgggaa tcatgctctt cttcgcgctt gatgcgctcg tagatctctt
     1861 ctctatgcac cgccacatcc ttgggcgcat tgactccgat ccggacttgg ttgcccttca
     1921 cgcctaatac ggtgaccgtg acgtcgttgc cgatcatcac ggtctcgccg acacgcctag
     1981 tcaatatcaa cataaatctg actcctagct ttctcgacat tgcagcgatc ctgaactgct
     2041 gctcgagatg ccgcatcgcc aatcggcggt cattcgcggc ttttgaaccg actatctcca
     2101 agaatacaag aatcccagcg gcaattgttg cctcttcagt cagaattgat cagaaataaa
     2161 caatccgttc aattagacag tcggctgcga accagggatt ccacgcccgc cagcgcccgc
     2221 cccaggtttt cgggttgagt accgcccgcc tgcgcaaaat ccgcgcggcc accgcccctg
     2281 ccaccgacct gggctgcgac cgcgccggca atatctcctg ccttcatgcg ggtcaacaaa
     2341 tccgccgaaa cgccggcaac cagcactacc ttgccgtcct gcaccgtagc caaaacgacg
     2401 accgaagatc ccaactttga ttttagctga tcgaccgcat cgcgcagcga cttggcatcg
     2461 gcgccctcga tctgagccgc taaaacctgg atcccgtcaa cattcttggc ctgagatgtt
     2521 aaatcaccgc cctggccggt cgcgagtttg gacttgagct gctgaacctc cttttccaac
     2581 ttgcgcgagc gttcaacaag ctcgcgaacc ttctcatcga cgtcatcgcg gctgccgcga
     2641 agcattccag ccacgtcgcg cagaacctgt tcggtgtgca caacccactc atacgccgtc
     2701 ttgccggtga tcgcctctac acgccgcaca ccggccgcaa cgcccgattc gctcaaaatc
     2761 ttgaacaatc caatatcgcc tgcgcgtttg acgtgagttc caccgcagag ttccagggag
     2821 aaatcgccga tggacagtac ccgcacatcg ctctcatatt tttcgccgaa tagcgacatg
     2881 gcgcctgccg ccaccgcatc gtccagcgcc atcacccggg tctcgaccgg cgcgttgctg
     2941 cgaatcgtcg aattcacgag ttcctcgacg cgctgcaatt cctcagcggt caggggctgc
     3001 gtatgcgaga aatcgaagcg caatcgctca gctgcaacca gcgatccctt ttgctcgacg
     3061 tgtgtgccca ataccttgcg cagggccgcg tgcaacagat gcgtcgcaga gtgattgaga
     3121 gcgatggaag tgcggcgctc gccgttgact tgcgcttcaa ctgcatcgcc gacccgcaga
     3181 atgcccgtgt cgagcactcc cacgtgcgca aacgaggcgc cgatcttctg agtgtcgcgc
     3241 acgataaagc gcgttgcgac gcgatcctgg cccaagcgaa ccaacacgcc cgcgtcgccg
     3301 atttgcccgc cgctctcggc ataaaacggc gtatggtcca agaccacctg gccttcctgg
     3361 ccgggcccga gcgtatcgac gacggcgccg tcgaaaatga gcgatgtcac cacaccgcgg
     3421 tcggccagac gatcgtagcc caaaaaatcc gtcttccccg cgagctttac ggagtcgcgc
     3481 aggtccgtgc cgaacttgct cgcggcgcgc gcccggccgc gctgcgcttc catcgccgcg
     3541 tcaaagcctg cctgatcgat actgagcccg cgctcgcgtg ccacatcggc agtgaggtcg
     3601 agcggaaaac cataggtgtc gtagagccga aacacggttt caccgggaat cactttggac
     3661 gtaagcttcg cggctgcggc gtcgagcagt gccattccgg tcgtcaacgt ctcggcaaat
     3721 cggctctcct cttgcgccaa aacctgggtc gcgcgcgcct cgccgctaat gagttcgccg
     3781 tagtagggcc ccatttcccg caccaagctc gccaccagct tgtggaagaa gggttgcgtt
     3841 tggcccaatt tatagccgtg gcgaattgcg cggcgaatga tgcgccgcaa aacatagccg
     3901 cggccttcgt tcgacggcac cacgccatcg acgatcaaga acgtgcaggc ccggatgtgg
     3961 tcggcgatga cgcgcaaact cgacgattcg aggtcgtgag ttcccgtgac ttctgcggcg
     4021 gaccggatca gcgacttgaa caggtcgatg tcgtagttgc tgtggacgcc ttgcatgacc
     4081 gcggccacac gctccaatcc catccccgtg tcgaccgatg gcttcggcag cggcaccaat
     4141 acaccgtccg acgagcggtc gtattgcatg aagaccagat tccagatctc cacaaagcgg
     4201 tcgccgtctt cgtcgggcgt gccgggcggt ccgcc
//
LOCUS       DASWZT010000090         8414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_58730, whole genome shotgun sequence.
ACCESSION   DASWZT010000090 DASWZT010000000
VERSION     DASWZT010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8414
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58730"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3510)
                     /locus_tag="VGE92_02345"
     CDS             complement(<1..3510)
                     /locus_tag="VGE92_02345"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEY1312682.1"
                     /translation="MRRALKVSGWVAAVGAMVILSLGGALLVAGNTSRGRVMIERLTY
                     RLTSGAVSLTGLAGSFPAQLTLERLELRDARGVWLSADRVSVSWSPLALLARRIQVDA
                     LHAQRIDMQRLPEAKPGAARDDTVSIPRIDAGTVVVDLATLGAELAGAPASLELRGSA
                     HLRSVQDMLIDATARRIDGDGHYSMQLRFDSKRMDAALEVHEPAGGPLQNLLQLPGLG
                     AVAARLSLHGPREAERLDLAIDAGAVRGRAQGSFNLKDLSADLAVDLDSKAQAPRADL
                     AWERASVHGRWHGSVKTPNADAHVELIQLRLPGGSQLASASGDLKAQSGTAQLHAVLS
                     GLRIPGPQPQLLADSPLTLDASVRLHEAARPLQLTLTHRLFALQASAETAAGKAGRRE
                     ASAQLRLPNAEQFAALAGVKMRGSALIKARLQDDAAATRVTLDASAAPQVGSEFWSQA
                     VGDSASLQLAASLTPRALTVESMKFSGRALALTASGELTRPAPDSKDTAVLRVGWDLN
                     VSDLKTLSPAIAGTAKASGKLQGPLAGFAADAQLSASLSVRNSPSGSLSATINMRGLP
                     SAPSGSLAAQGMLDGAPVDVDVVVERAQAGAVRAVIHRAAWKSVHADGDINVAPAFAQ
                     SHGQLNLQMGQLGDLQDLLGVKLAGSLAGTVSVHPGQGHTEARVQIDARDVTAGAFVG
                     NAQLTAEGGTEALGFKLELQAPNVNGAKASLSAAGSLDVDARRIDLTRAVANYRGQDL
                     SLLTPAHIALANGVAVDDLRIGAQEAVLELKGEISPALDASASLLKVQPALVNVFVPG
                     LLASGTIEAHAQLQGSLSSPTGEVRFLATDTRLGDDSAFGLPPLDARANAQLQGDSAQ
                     IDAHLVAGTSKLNLTGRAPLGADGSSDLKINGAVDVGIINPLLEARGQHAAGDLNVDA
                     TVTGNVAQPQIAGTVNLSKGSFRDYARGVSLSDISAAIVGSAGNLEIKTFTAAAAPGT
                     VSMTGKVGVLQAGIPVDLHIKADRAQPLVSKLVTSNFNADISVSGKARERLDVAGTVL
                     LNRTLIGIPNGMPSNVAVLDVRRHGKTAPAPVDKPLVVNLDVTLQAPQEILVQGRGLD
                     AEMGGDLHLGGTTDTPLVSGGFDLQRGNFSIAGNKLNFTAGRVSFNGAGLKNKIDPTL
                     DFTAQAAVTDVTATLRITGLADAP"
     gene            complement(3510..5354)
                     /locus_tag="VGE92_02350"
     CDS             complement(3510..5354)
                     /locus_tag="VGE92_02350"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEY1312683.1"
                     /translation="MTQTALALLFAAVGFACLAHAADPQPYKVTIASVGNSEMDATLK
                     ATSDLQGLRSTAPVSPFGLIARARSDVDRLKTALESFGYYRSSVAIKIDGMLVSNPLL
                     GDQLTALAKGTDAQVAISFTLGPLYHLRNIEVEGNLPEGIRDTLGLTTGQPAVASAVL
                     DGSSRMLGAMQERGYAFATVDPPVAYEAADAPVLDLHFRVATGTKVNIGEIHFEGLQR
                     LREPLLRGRILMRTGQLYRPSVIERARRDLLALGVFSQVSLQVGTAVDSSGGVPITFK
                     VRERLRHAITVNAAYSSDLGGSGGVTWTDRNVFGNAEQLSVAASVINLGGSDTTGIGY
                     DTSIKYLIPDVLRRDQSLQFAVGALKQSLQAYDQTARTTSVTLTRKFSSVWSASIGGA
                     TSEEQIIQEGNTYNYTLFAIPMTVSYDSTDLASPLEDPRHGIRSSLGVTPTFALGTQN
                     ATFIITQIKAATYFDLDKLLAVGPGRTVLAARALAGIAEGAGEFSLPPDQRFYAGGSG
                     TVRGYGYQLVGPLFADGTPRGGTAITAGSVELRQRFGTNFGAAVFVDAGQVSANLKFV
                     PQQLRVGTGVGLRYFTPIGPIRLDVAVPTKRGPYDDAFEVYIGLGQAF"
     gene            5492..5710
                     /locus_tag="VGE92_02355"
     CDS             5492..5710
                     /locus_tag="VGE92_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312684.1"
                     /translation="MGNANTDPTGEDKLRGGALTGSEQKNAADHISYEKSRNPDSVVR
                     VDNEEDTLYSDGLELEDHTPPLGTDNAL"
     gene            complement(5739..6545)
                     /locus_tag="VGE92_02360"
     CDS             complement(5739..6545)
                     /locus_tag="VGE92_02360"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY1312685.1"
                     /translation="MLVSGLAHAIVSAILKAGKDKMSGRALIDGFSAVLIAPAALIVP
                     LPVHAWGWLFASWLVHLLYLLALIKSFEQSDMTVAYPIARGVAPLLAAAAAVAIFHEP
                     MNGYVAFGIALITLGVMCIGVSHTMNRRALLWALLTGGCIAGYTVIDAQGVRAAPSGA
                     SYIVWTFILLGGGISSIFAIWRGRAFFASIAGQWRPGLAAGALSIVSYGVALLAFRFG
                     TTPRLAALRETSILFGTVIAVAFLHERLTRLRYAGVIGIAIGAITLLAGR"
     gene            6759..7196
                     /locus_tag="VGE92_02365"
     CDS             6759..7196
                     /locus_tag="VGE92_02365"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY1312686.1"
                     /translation="MGELNNSLAPLLNQVRIAMLGGVDQEFLLDADVAPLEVTAAQFV
                     IIANVLKGHANSACELCKFMDYDRGAMSRMIDRLETKGLIRRVALAHTRRTVALEVTA
                     EGKAAFPKMQACLNRVLDRLLKGVSKTQVREVEKTLKRMLANS"
     gene            7282..7455
                     /locus_tag="VGE92_02370"
     CDS             7282..7455
                     /locus_tag="VGE92_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312687.1"
                     /translation="MLDFIESNAGRIAAGLQTIGGSFDPEVLALPGGSIIAVVLWLHA
                     GAVHTWKRSSCGS"
     gene            7443..7604
                     /locus_tag="VGE92_02375"
     CDS             7443..7604
                     /locus_tag="VGE92_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312688.1"
                     /translation="MRLLMAGAFLIALGMLVLIGFLRLLRELVAKDFFAEPSIHKEAA
                     DQGGSNVDI"
     gene            7709..8308
                     /locus_tag="VGE92_02380"
     CDS             7709..8308
                     /locus_tag="VGE92_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY1312689.1"
                     /translation="MSSDKSRVRDRIFQTACELFYRHGIHGVGVDAIAAEAGSNKMSF
                     YRSFASKDALVAEYLRQQERGYWAWWEATVARFPDDPRRQVEALFESQLEMAMANNCR
                     GCALGNAAAEISDEEDALSAMVLAYKEQVRSRLHAMAHAMGAHDADNLGDALMLLLDG
                     GYFTRLVFPRGSGPIAAALPAVRALIEAHLPPERRPTPA"
BASE COUNT         1529 a         2735 c         2671 g         1479 t
ORIGIN      
        1 cggcgcatcc gcaagtcccg tgatgcgcag cgtcgcggtc acatcggtca ccgcggcctg
       61 cgccgtgaag tccaaagtcg gatcgatctt gttcttgagc cccgcgccat tgaagctgac
      121 gcggcccgcc gtgaagttca gcttgttgcc ggcgatggag aaattgccgc gctgcagatc
      181 gaaaccgccg ctcaccaggg gcgtgtcggt cgtgccgccg aggtgtaggt ctccgcccat
      241 ctccgcatcc aagccgcgtc cctgcacgag aatctcttgc ggcgcctgca gcgtcacgtc
      301 gaggtttacg accaatggct tgtcgaccgg cgccggtgca gtcttgccgt gccggcgcac
      361 atcgagaacg gcgacattgg acggcatgcc gttcggaatg ccgatcaacg tgcgattcag
      421 aagcaccgtg ccggcgacgt ccagccgctc gcgggccttg ccgctcacgc tgatatccgc
      481 attgaagttc gaggtcacca atttgctcac caagggttgc gcacggtccg ccttgatgtg
      541 caggtcgacc gggatacccg cctgcagcac gccgacctta cccgtcatcg acacggttcc
      601 cggcgccgcc gccgcggtaa atgtcttgat ttcgagattt cccgcgctgc cgacgatcgc
      661 ggcgctgatg tcgctgaggc taacgccgcg cgcgtaatcc cgaaagctgc ccttgctcaa
      721 attgaccgtg cctgcgatct gcggctgcgc tacgttgccg gtcacggtgg catcgacgtt
      781 aagatcgccc gccgcatgtt ggccgcgcgc ttccagcaag ggattgatga tcccgacgtc
      841 gacggcgccg ttgattttca ggtccgacga gccgtcggct cccaacggcg cacgcccggt
      901 gaggttcagt ttcgaggtac ccgccacgag gtgcgcgtcg atttgcgcgc tgtctccctg
      961 caactgcgcg ttcgcacgtg catcgagcgg cggcagcccg aaggccgaat cgtcccccag
     1021 gcgcgtgtcg gtcgccagga atcgtacctc gccggtgggt gagctcaagc ttccctgcag
     1081 ctgcgcgtgc gcctcgatgg tgcccgacgc caacaatccg ggaacgaaca cgttgaccaa
     1141 cgcaggctga accttgagca gcgaggcgct cgcatcgagc gccggcgaga tttcgccctt
     1201 gagctcgagc accgcctcct gtgctcctat ccggaggtca tcgaccgcga cgccgttcgc
     1261 caatgcaatg tgcgccggcg tcagcagact caggtcctgt ccgcggtaat tcgcgacggc
     1321 acgcgtcaga tcgattctgc gcgcatcgac atccaacgaa ccggctgccg agaggctcgc
     1381 tttggcgccg ttcacgttcg gcgcttgcaa ttcgagcttg aatccaagcg cctcggtgcc
     1441 gccttcggcc gtcaactgag cgttgccgac gaacgctccc gcagtcacgt cgcgtgcgtc
     1501 gatctgcacc cgtgcttcgg tgtgcccctg acccggatgg acgctgacgg tccccgcgag
     1561 gctcccggca agcttgaccc cgagcaagtc ctgcaggtcg ccgagctgcc ccatctgcaa
     1621 gttcaattga ccatggcttt gcgcgaaggc cggcgcgacg ttgatgtcgc cgtccgcgtg
     1681 cacgctcttc catgcagccc gatgaatgac ggctcgcacc gctccggctt gagcgcgttc
     1741 caccacgaca tcgacgtcaa ccggtgcgcc gtcgagcatt ccctgcgccg cgaggctgcc
     1801 gcttggtgcc gacggcaagc cgcgcatgtt gatcgtcgcc gagagcgagc cgctcggcga
     1861 attgcgcacc gacaggctcg cactcaactg tgcatccgcg gcgaatccgg cgaggggccc
     1921 ttgcagtttt cccgacgcct tggcggtgcc ggcgattgcc ggcgacaggg ttttcaaatc
     1981 cgacacgttg aggtcccagc caacgcgcag aaccgccgta tccttcgaat cgggcgccgg
     2041 ccgcgtgagc tcaccgctcg cggtcaaggc cagcgcgcgg ccgctgaact tcatgctttc
     2101 gacggtgagg gcacgcggcg tgagcgatgc ggccagttgc aaactggcgc tgtcgccgac
     2161 agcctgcgac caaaattcgc tgcccacctg cggcgcggcg ctcgcatcga gcgtcacgcg
     2221 cgtcgctgcc gcgtcgtcct ggagccgcgc cttgatgagc gcgctgcccc gcattttgac
     2281 gccggcgagc gcggcgaatt gctctgcatt cggcagtcgt agctgcgcgc tcgcctcgcg
     2341 tcggccggcc tttcccgcgg ccgtttcggc cgatgcctgc agcgcgaaca agcgatgcgt
     2401 cagcgtcaat tgcagcggcc gcgccgcctc atgcagccgc acggatgcat cgagtgtcag
     2461 cggcgagtcc gccaataact gcggctgcgg tcccggaatt ctcaggccac tgagcaccgc
     2521 atgcagttgg gcggtccccg actgcgcctt cagatcgccg ctcgccgacg ccaattgcga
     2581 gcctcccggc agtcgcaatt gaatgagctc gacgtgagca tccgcattcg gcgtcttgac
     2641 gctgccgtgc catcgcccat ggacgctggc gcgctcccac gcaagatccg cgcgcggtgc
     2701 ttgggccttg gaatcgaggt cgacagcaag atcggccgac aagtctttca ggttgaagct
     2761 gccctgcgcg cggccgcgca cggcaccggc atcgattgcc aggtcaagac gctcggcctc
     2821 gcgcggcccg tgcaggctca agcgcgcggc cacggcgccg aggcccggca attgcagcaa
     2881 attctgcagc gggccacccg caggttcatg cacctccaag gcagcgtcca tccgtttcga
     2941 atcgaaacgc agctgcatcg agtaatgtcc gtcgccgtcg atgcgccggg ccgtggcgtc
     3001 gatcagcatg tcctgaaccg aacgcagatg cgcgctgccg cgtagctcga gcgaggccgg
     3061 cgcgccggca agctccgctc cgagcgtggc cagatccacc accaccgtgc cggcgtcgat
     3121 gcgcgggatg gatacggtgt cgtcgcgcgc ggcgccaggc ttcgcttcgg ggagtctctg
     3181 catatcgatg cgttgcgcgt gcaacgcatc gacttgaatg cgccgcgcca ggagcgcgag
     3241 cggcgaccag ctcaccgaca cgcggtcggc gctgagccac accccccgtg catcgcgaag
     3301 ttccagacgc tcgagcgtca gctgcgccgg aaatgagccc gccagaccgg tcagcgaaac
     3361 cgcgccggag gtcaagcggt aggtgagccg ctcgatcatg acgcggcctc gcgaggtatt
     3421 gccggcgacg agcagcgcgc cgcccaacga caagatcacc atcgcaccga ccgccgccac
     3481 ccatcccgat actttgagtg cgcggcgcat cagaacgctt gacccagtcc gatgtacacc
     3541 tcgaaggcgt cgtcatacgg accgcgtttg gtgggcacgg caacatccag gcgaatgggt
     3601 ccgattggcg taaagtagcg caatccgaca cccgtgccga cgcgcagctg ctgcggtacg
     3661 aacttgaggt tcgcgctgac ttgtcccgcg tcgacgaaca cggcggcgcc gaagtttgtg
     3721 ccaaagcgct gccgcaattc cacgctgccg gcggtgatgg cggtgccgcc tcgcggcgtg
     3781 ccgtcggcga aaagcggacc caccaattgg tacccgtagc cgcgaacggt cccgctgccg
     3841 ccagcgtaga agcgctgatc cggcggcaaa ctgaattcgc ccgcgccctc ggcaatgccg
     3901 gcgagcgcgc gcgccgcaag tacggtacgg ccgggcccga ccgccagaag tttatcgaga
     3961 tcgaaatacg tggcggcctt gatctgggtg atgatgaagg tggcgttctg agttcccaga
     4021 gcaaacgtag gcgtcactcc gagcgaggaa cgaattccat ggcgcggatc ctcgagcggc
     4081 gaggcaagat cggtggagtc gtacgacacc gtcatcggta ttgcaaacaa cgtgtagttg
     4141 tacgtgttgc cctcttgaat gatctgttcc tccgacgtcg ccccgccgat gctggcgctc
     4201 cacacgctgg agaacttgcg ggtcagggtc acgctggtgg tgcgcgccgt ttgatcatag
     4261 gcctgcaagg attgcttgag tgcgcccacg gcgaactgca gcgactggtc gcggcgcagg
     4321 acatccggta tgaggtactt gatgctcgtg tcgtagccga tgcccgtggt atcgctgccg
     4381 cccagattga tcaccgaggc tgccacgctc aattgctcgg cgttgccaaa cacattgcgg
     4441 tcggtccacg tgacgccgcc gctgccgccg aggtcgctgg agtacgctgc attgacggtg
     4501 atcgcatggc gcaagcgctc gcgcaccttg aaggtgatcg gcacgccgcc ggaactatcg
     4561 accgcggtgc cgacctgcaa actgacctgg ctgaacacgc ccaacgccaa caggtcgcgg
     4621 cgcgcccgct cgatcaccga ggggcggtac agttggccgg tacgcatcaa gatcctgccc
     4681 cgcagtaacg gctcgcgtag acgctgcaag ccttcgaagt ggatctcgcc gatgttcacc
     4741 ttggttcccg tcgcgacccg aaagtgcagg tcgagcacgg gcgcatccgc ggcctcgtag
     4801 gccactggcg gatccacggt agcgaaggca tagccccgtt cctgcatcgc gcccagcatg
     4861 cgcgagctgc cgtcgagcac cgccgacgca accgccggct gcccggtcgt caggccgagc
     4921 gtgtcgcgta tcccctcggg taggttccct tcgacctcga tgttgcgcaa atggtagagc
     4981 ggccccaggg tgaagctgat cgccacctgc gcatccgtcc ccttcgcgag cgcagtcagt
     5041 tgatcgccga gcagcggatt gctcaccaac atgccgtcga tcttgatcgc cacgctgctc
     5101 cggtagtagc cgaagctctc gagcgcggtc ttgagccggt cgacgtcgct gcgcgcgcgc
     5161 gcgatcaggc cgaacgggct gaccggcgcc gtgctgcgca agccttgcaa atccgaagta
     5221 gccttgagcg ttgcatccat ctcgctgttt cccaccgagg cgattgtgac cttgtacggc
     5281 tgcggatccg ctgcatgggc gagacaggca aagcccactg cagcaaacag aagcgcgagt
     5341 gcggtttggg tcattcgctg cgaaggataa ctgatgtgca aatgggcttt tgtcggctaa
     5401 agactacaag ccgtgcgtat cgcgcacgga tatccgcgcc ctgcggttgc gcgatagctt
     5461 gcaggtctca tattactcac tggagttaac gatgggcaac gcaaataccg atccgaccgg
     5521 cgaggataag ctgcgcggcg gcgccttgac cggttcggaa cagaaaaacg ccgcagatca
     5581 tatttcttac gagaagtcgc gcaatccaga cagcgtggtg cgggtcgaca acgaagagga
     5641 cacgttgtat tccgacggcc tggaactcga agaccatacg ccgccgctcg gtaccgacaa
     5701 cgccctttag gttgatgcac gcgcgccggc cgaacgcatt agcgacccgc gagcagcgtg
     5761 atcgcgccga tggcgatgcc gatgacccct gcgtacctga gacgcgtcag acgctcgtgt
     5821 aaaaaggcca cggcgatgac ggtaccgaac aagatcgacg tttcgcgaag cgccgcaaga
     5881 cgcggggtgg tgccaaagcg aaatgccagc agcgccaccc cgtagctgac gatcgacagt
     5941 gcgccggcgg ccaggcccgg ccgccattgg ccggcgatcg aggcaaagaa ggctctgccg
     6001 cgccaaattg cgaatattga gctgatgccg ccgcccagca gaatgaaagt ccagacaatg
     6061 tagctcgcgc cgctcggcgc cgcgcgtacc ccttgcgcat cgatgaccgt atagcccgcg
     6121 atacagcctc ccgtcagcag cgcccataac agggcgcgcc gattcatcgt gtggctgacc
     6181 ccgatgcaca tcacgccgag ggtgatcaaa gcaatgccga aggccacgta gccgttcatc
     6241 ggttcatgaa atatggcgac cgcggcggcg gccgcgagca gcggggcgac cccgcgcgcg
     6301 atcgggtagg cgaccgtcat atccgattgt tcaaaggact tgatcagtgc gagcaagtac
     6361 aaaagatgca ccaaccagga agcgaacagc cacccccagg cgtgaacggg caacggcacg
     6421 atcagcgccg ccggcgcaat caacacggcg ctgaatccat cgatcagcgc acgccccgac
     6481 atcttgtcct ttcccgcctt gagtatggcg ctgacgatgg cgtgggcgag tccggaaacc
     6541 agcattaaca gcgaggcggt actctccatc ggctagcggc gcagaacgac aatcacgcgg
     6601 ctgggagagc gagcatgctg cgcactgtag atgtattcgc cgcagaatgg cagcggcagc
     6661 gcgcgccggc gcggcgtcga tcaccgtccc ctgctgcctt gacaattgtt gcctcggcaa
     6721 ctatactcgc tgcatgagga tctcgtgcgg cgtgtgtgat gggtgaactc aacaacagtc
     6781 tggcaccttt gttgaaccag gtgcggatcg cgatgctcgg cggcgtcgat caggagttcc
     6841 tgctcgatgc cgatgtggcg ccgctcgaag tgacggccgc gcaattcgtc atcatcgcca
     6901 atgtcctcaa agggcacgcg aattccgcgt gtgaattgtg caagttcatg gactacgatc
     6961 gcggcgccat gagccgcatg atcgaccgcc tcgaaaccaa gggcttgatt cgccgtgttg
     7021 cgctcgcgca tacgcggcga accgtggcgc tcgaggtgac ggcggagggc aaagcggcgt
     7081 ttccgaagat gcaggcctgt ctcaaccggg ttctcgaccg gttgctgaag ggggtgagca
     7141 aaacccaggt gcgcgaagtc gagaagacgc tgaaacggat gctcgcgaac agttgatttt
     7201 ttttggttta atggttgccc aggcatttgt tgttcaggca gctgccggga gggacttcga
     7261 gtcccgctga ggagaggtgc aatgttggat ttcatcgaat cgaacgccgg cagaattgcc
     7321 gccgggctgc aaacgatcgg cggcagtttc gatcccgagg tattggctct gccgggcggc
     7381 agcatcatcg ccgtagtcct gtggctgcat gcaggcgccg tgcacacctg gaagcgcagc
     7441 tcatgcggct cctgatggca ggcgcgttcc tgatcgcgct cggcatgctc gtactgatcg
     7501 gatttttgcg tttgctgcgc gaactcgtgg ccaaggactt tttcgccgag ccctcgatcc
     7561 acaaggaggc ggccgatcag ggcggctcga acgtcgacat ctagagcttg acttgaaacc
     7621 caccgacggc gaccctatgt agggagttac cgattagtaa catttgtcag ttagcgatcg
     7681 cagtcacggc tgacaacggt gggcgggcat gagtagcgac aaatcgcgag tgcgcgaccg
     7741 tatcttccaa acggcttgcg agctgtttta ccgacacggc atccacggcg tcggagtcga
     7801 tgccatcgcc gccgaagcgg gctccaacaa gatgagcttc tatcgcagct tcgcctccaa
     7861 agatgcgctg gtcgccgagt atttgcgcca gcaagaacgc ggctattggg cttggtggga
     7921 agccacggtg gcgcgatttc cggatgatcc gcgccgccag gtcgaagcac tcttcgaatc
     7981 acagctcgag atggcgatgg cgaacaactg ccgcggctgc gcgctcggta acgccgccgc
     8041 ggaaatctcc gatgaggaag atgccttgag cgccatggtg ctcgcctaca aagagcaagt
     8101 gcgctcgcgg ctacatgcca tggcgcacgc catgggcgcg catgacgccg ataacctcgg
     8161 agatgcattg atgctgctgc tggacggggg gtatttcacc cggctggtct ttccgcgcgg
     8221 tagcggcccg atcgcggctg cgctgcctgc ggtgcgcgca ctcatcgagg cccacctgcc
     8281 gcccgagcgt cgaccaacgc ccgcgtaatt tgcggcggcg agcagcgtgg ccgcccgcta
     8341 agccgtggca aactcggtgc ggcgttctgc gacccatcgc cagagtgagg cgagcggcgc
     8401 gatcaacccg gcgc
//
LOCUS       DASWZT010000091         4641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_39480, whole genome shotgun sequence.
ACCESSION   DASWZT010000091 DASWZT010000000
VERSION     DASWZT010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39480"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1389)
                     /locus_tag="VGE92_02385"
     CDS             complement(<1..1389)
                     /locus_tag="VGE92_02385"
                     /inference="COORDINATES: protein motif:HMM:NF015185.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1312690.1"
                     /translation="MNLVSGKLAKKWMQLGTEFLPFADAASAELPLSRLLRLSLFQVS
                     VGMAMVLLIGTLNRVLIVELSVPAWLVSLMVGLPLVFAPFRAFIGFRSDNHRSALGWR
                     RVPYIWMGTLLQFGGLAIMPFALLILSGDTHGPIWIGQFASAFSFLLVGAGLQTTQTA
                     GLALATDLAPTESRPSVIALMYVMLLVGMVAAGLVFGRLLANFTEIRLIQVVQGAAMV
                     TMALNIAALWKQEARHSARSALAVSGARFLDSWRQFTQHARAIRFLVTVGLGTAAFNM
                     QDIVLEPYGGEILHLPVGATTALTAMLAGGMLAAFSLAARFLGHGADPYRLAAYGTLI
                     GLVAFAAVIFAAPMESPLLFRLGVLMIGFGGGFFSVGTLAGAMARERNGESGLALGAW
                     GAVQATSAGVAIALGGAVRDAVTGLAVHGSLGPALSGASTGYGAVYQIEIVLLFATLV
                     AIGPLVRSGPKVA"
     gene            complement(1386..2087)
                     /gene="bchM"
                     /locus_tag="VGE92_02390"
     CDS             complement(1386..2087)
                     /gene="bchM"
                     /locus_tag="VGE92_02390"
                     /EC_number="2.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR02021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046406 - magnesium protoporphyrin IX
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium protoporphyrin IX methyltransferase"
                     /protein_id="HEY1312691.1"
                     /translation="MMTLSYQRRRGELETYFDRTAVDAWARLTSTAPVSKIRATVRAG
                     RDQMRSTLLQMLPADLQGQRLLDAGCGTGALSVGAAQRWAQVVAVDLSPTLVNLARER
                     LPAQLGFGSIEFRVGDMFDTSLGEFDHVVAMDSLIHYQLHDVVRVLAGFAAHARRSIL
                     FTFAPKTPALAVLWAAGRLFPRRDRAPAITPIAEPCMRRALAQEPALRHWRVGRTQRV
                     TSGFYKSQAMELVKL"
     gene            complement(2087..3004)
                     /gene="bchL"
                     /locus_tag="VGE92_02395"
     CDS             complement(2087..3004)
                     /gene="bchL"
                     /locus_tag="VGE92_02395"
                     /EC_number="1.3.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin:protochlorophyllide reductase
                     (ATP-dependent) iron-sulfur ATP-binding protein"
                     /protein_id="HEY1312692.1"
                     /translation="MTIATATVPVSALNYRVRPPDGAGSVQVPLDPNLKIGNAKVFAV
                     YGKGGIGKSTTSSNLSVAFTKLGKRVLQIGCDPKHDSTFTLTKRLVPTVIDVLESVNF
                     HSEELRVEDFVYQGYNGVMCVEAGGPPAGTGCGGYVVGQTVKLLKEHHLLEDTDVVIF
                     DVLGDVVCGGFAAPLQHADRALIVTANDFDSIFAMNRIVAAIQAKSKNYAVRLGGVIA
                     NRSAGTDQIDKFNTRVGLKTMARFPDLDVVRRSRLKKATLFEMERSPELDAVQAEYLR
                     LAQALWDGTDPLIAESLKDREIFDLLGFD"
     gene            complement(3001..>4641)
                     /locus_tag="VGE92_02400"
     CDS             complement(3001..>4641)
                     /locus_tag="VGE92_02400"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HEY1312693.1"
                     /translation="LDLAELEPAWGGAADASIARLGAQILELEYTLIPHGLHVVGRAA
                     TPLERVDFLMAMAQSASAAAAANPSALRSAVLAVMDGSPAEQALAAAGLPACEADVTL
                     LGELRTADRMLVDNGEIEAIVHALDGGFLRPAPGGDIVRTPTVLPTGRNLHGFDPFRI
                     PSAFAMLQGAKQAKQLIERHLAAGNPLPESVALVLWGTDNLKTEGGPIGQALALIGAR
                     PRFDAYGRIAGATLIPLEELGRPRIDVMVTLSGIFRDLLPLQIKLLAEAAFLAASADE
                     PVQQNFVRKHTLHYQREHACDLETAALRVYGNAESSYGSNVNHLIENGRWDEPDELAE
                     TYTRRKSFAYGRGGRPIPQAKLLSSVLADVQLTYQNLDSVELGVTTVDTYFDTLGGIN
                     SAVRRAKGGVATPVYIADHTSGSGTVRTLAEQVGLETRTRTLNPKWYEGMLEHGYEGV
                     RQIEAHVTNTMGWSATTGEVQPWIYQQITQTFILDPQMRARLAKLNPTASTKVANRLL
                     EASERKYWQPDEQMLAALRAAGEELEDRLEGIYEGVAA"
BASE COUNT          892 a         1505 c         1448 g          796 t
ORIGIN      
        1 ggccaccttc ggtcccgacc gcaccagcgg tccgatcgcc accaaggtgg cgaacaacag
       61 aacaatttcg atttgataaa cggcgccgta gccggtcgag gcgcccgaca gcgcgggccc
      121 gagcgaaccg tggaccgcaa gaccggtgac ggcgtcgcgg accgcgccgc ccaatgcaat
      181 cgccactccg gcgctggtcg cctgcactgc tccccaagcg cccagcgcaa gaccgctctc
      241 gccgttgcgc tcgcgcgcca tggcacccgc cagcgtcccg accgagaaga atccgccgcc
      301 gaagccgatc atgagaacgc cgagccgaaa cagcagcggc gactccatgg gcgcggcgaa
      361 gatgaccgcg gcaaatgcaa ccagtccgat gagcgttccg taggccgcga gacggtaagg
      421 gtcggcgcca tgtcccaaga atcgagcggc gagcgagaat gcggccaaca tgccgccggc
      481 gagcatcgcc gtcaacgccg tggtcgcgcc gaccggcaga tgcaggatct cgccgccgta
      541 cggctcgagc acgatgtctt gcatgttgaa tgcggctgtc cccaatccca ccgtcaccaa
      601 gaaacgaatt gcgcgtgcgt gctgggtgaa ttggcgccac gagtcgagga atctggcgcc
      661 gcttaccgcg agcgcgctac gcgccgaatg ccgcgcctcc tgcttccaca gtgccgctat
      721 gttcaaggcc atggtcacca ttgctgcgcc ctgaaccact tggatgaggc ggatctcggt
      781 gaagttggcg agcagcctgc cgaacaccag gccggcggcg accatgccga ccagcagcat
      841 gacgtacatc aatgcgatca cgctcggcct cgactcggtc ggcgccaagt cggtggcgag
      901 cgcaagaccg gcagtttgcg tggtctgcaa gccggcgccg accagcaaga acgagaacgc
      961 ggatgcgaat tgtccgatcc aaatcggacc atgggtgtcg ccggacagga tcaacagcgc
     1021 gaacggcatg atggccaggc cgccaaattg cagcagcgtg cccatccaga tgtaaggtac
     1081 gcggcgccag cccaaggccg agcgatgatt atcggagcga aagccgatga acgcgcggaa
     1141 tggtgcgaag accagcggca aaccgaccat caaagacacc agccacgcgg gcacgctgag
     1201 ttcgacgatc agcacgcgat tgagggtccc gatcagcagc accatcgcca tgccgaccga
     1261 tacttgaaac aaggacaagc gcaacaggcg gctcaaaggc agttcggcgc tcgccgcgtc
     1321 cgcaaacggc agaaactcgg tacccagttg catccatttt ttcgcgagtt tgccgctgac
     1381 gagattcata gcttgacgag ctccatggcc tgcgatttgt aaaagccgct ggtgacgcgc
     1441 tgcgtgcgcc caacccgcca gtgacggagc gcaggctctt gcgccaaagc acgccgcatg
     1501 caaggttcgg caatcggcgt gattgccggc gcgcggtcgc ggcgcggaaa cagcctgccc
     1561 gcggcccaca gcacggcaag agcgggagtc ttcggcgcga aagtgaacag tatcgagcgc
     1621 cgcgcgtgcg ccgcaaagcc ggccagcacc cgcaccacat cgtgcaattg ataatgaatg
     1681 agagaatcca tggcgaccac gtgatcgaat tcaccgaggc tcgtgtcgaa catgtcgccg
     1741 acgcgaaact cgatgctgcc gaagccgagt tgagccggca atcgctcgcg cgccaggttc
     1801 accagggtgg gtgataggtc gacggcgaca acctgcgccc agcgctgcgc cgcgccgacg
     1861 ctgagcgcac cggtcccaca tcctgcgtcg agcagccgct ggccttgcaa gtccgcgggc
     1921 aacatctgca gcagcgtgct gcgcatctgg tcgcggccgg cgcgcaccgt tgcgcgaatc
     1981 ttgctcaccg gcgcggtcga ggtgagccgc gcccaggcat cgactgcggt ccgatcgaag
     2041 taggtctcga gctcgccgcg ccgccgttga tagcttaggg tcatcatcag tcgaatccca
     2101 acaaatcgaa gatctcgcga tctttgaggg actccgcgat caacggatcg gtaccgtccc
     2161 acaacgcctg cgccagccgc aagtattcag cctgtacggc atcgagctct ggagagcgtt
     2221 ccatctcgaa caacgtggct tttttcagcc ggctgcggcg caccacatcc agatccggaa
     2281 accgtgccat ggtcttgagt ccgacgcggg tattgaattt gtcgatttga tcggtgccgg
     2341 cgctgcggtt ggcaatgacg ccgccgaggc gcaccgcata attcttggat ttcgcctgaa
     2401 tcgccgccac gatccgattc atcgcgaaga tcgaatcgaa atcgttggcc gtgacgatca
     2461 gcgcgcggtc cgcgtgctgc agcggcgccg cgaatcctcc gcacacgaca tcgccgagca
     2521 catcgaagat caccacgtcc gtgtcctcga gcaggtgatg ttccttgagg agctttacgg
     2581 tctggccgac gacgtagccg ccgcagccgg ttcccgcggg ggggccgccg gcctcgacgc
     2641 acatcacgcc gttgtagcct tgatagacga agtcctcgac gcgcagttct tcggaatgaa
     2701 aattcaccga ctcgagcacg tcgatcacgg tcggcaccag acgcttggtg agggtgaacg
     2761 tggagtcgtg tttcggatcg cagccgatct gcaagacgcg cttgccgagc ttggtgaacg
     2821 ccaccgacag attggaggac gtggtgctct tgccgatgcc gcctttgccg tagaccgcga
     2881 acaccttggc attgccgatc ttgaggtttg gatcgagcgg aacctgcacg ctgccggcgc
     2941 catccggcgg cctgactcga tagttgagcg ccgataccgg gacggtggcg gtggcaatgg
     3001 tcatgcggct accccttcgt atatgccttc gagtcgatct tcaagctctt caccggccgc
     3061 gcgcagcgcc gccagcatct gctcatcggg ttgccagtat ttgcgctccg aggcttccaa
     3121 caagcgattg gccaccttgg tcgatgcggt tggattgagt ttggcgaggc gcgcccgcat
     3181 ctgcgggtcc aaaatgaacg tctgggtgat ttgctgatag atccagggct gcacttcgcc
     3241 cgtggtggca gaccatccca tggtattggt gacatgcgcc tcgatttggc gaacgccttc
     3301 gtagccgtgt tcgagcatcc cctcgtacca cttggggttc aacgtgcgag tgcgcgtttc
     3361 caagccgacc tgctccgcca aggtgcgaac ggtgccgctg ccgctggtgt ggtccgcaat
     3421 ataaaccggt gttgcgacac cgcctttcgc gcgccgcaca gcgctattga tgccacccaa
     3481 ggtatcgaag tacgtgtcga ccgtggtcac cccaagttcc accgagtcga gattctgata
     3541 agtcagctgc acgtcggcga gcacgctgct caagagcttc gcctgcggga tggggcggcc
     3601 gccgcggccg tacgcgaagc tcttgcgacg cgtgtacgtc tcggcaagct cgtctggctc
     3661 atcccatctg ccgttttcga tcaagtgatt gacgttcgat ccgtagctgc tctcggcgtt
     3721 tccgtagacg cgcaaggcag ccgtttccag gtcgcaagcg tgctcgcgct gataatgcag
     3781 cgtgtgtttg cgcacgaagt tctgctgcac gggttcgtcg gcggacgcgg ccaagaatgc
     3841 agcctcggcg agcaacttga tttgcagcgg cagcagatcg cgaaatatcc ccgacagggt
     3901 gaccatgaca tcgatgcgcg gacggcccag ctcctcgagc ggaatcaaag tcgcgcctgc
     3961 gatccgccca tacgcgtcga agcgcggccg tgcgccgatc aaagccaacg cctgcccgat
     4021 cgggccgcct tcggtcttca aattgtcggt accccacagt accaatgcca cggattccgg
     4081 caatggattg cccgccgcca ggtggcgctc gatgagttgc ttggcttgct ttgccccttg
     4141 caacatcgcg aaggcgctcg ggatacggaa gggatcgaag ccgtgcaaat ttcgccccgt
     4201 cggcaggacg gtcggagtcc gaactatgtc gccgccgggc gccgggcgca ggaagccacc
     4261 gtcgagcgcg tgcacgatcg cctcgatttc accgttgtcg acgagcattc gatcggccgt
     4321 ccgcaactcg ccgagcagcg tcacatccgc ctcgcaggcg ggcaggcctg cggcagcgag
     4381 cgcctgctcc gcgggactgc cgtccatcac cgccagcacg gcgctgcgca gtgcactggg
     4441 atttgccgca gcagcggcgc tcgcggattg cgccatcgcc atcagaaagt cgacacgctc
     4501 cagcggcgtt gccgcacgtc cgaccacgtg caacccgtgg ggtatcaagg tgtactcgag
     4561 ttccaggatc tgggcaccga gcctggcgat cgacgcatcg gcagcgccgc cccacgcggg
     4621 ctcgagttcg gccaggtcga g
//
LOCUS       DASWZT010000092         4602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_15177, whole genome shotgun sequence.
ACCESSION   DASWZT010000092 DASWZT010000000
VERSION     DASWZT010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15177"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VGE92_02405"
     CDS             complement(<1..256)
                     /locus_tag="VGE92_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312694.1"
                     /translation="MRRFNRSMGGWVAVAAIACAPVAPALAGGHGFGHAHVWGFGHGL
                     LGAVVGLATLPLVVASAAISAATPDVGYPSAGYDAGARGYA"
     gene            472..885
                     /locus_tag="VGE92_02410"
     CDS             472..885
                     /locus_tag="VGE92_02410"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEY1312695.1"
                     /translation="MNLKGSKTEKNLKDAFAGESQANRRYLYFAAKADVEGFNDVSAV
                     FRSTAEGETGHAHGHLEYLEAVGDPATGLPIGATSLNLKAAIAGETHEYTDMYPGMAK
                     SARDEGFGEIADWFETLAKAERSHANRFQKALDNL"
     gene            972..2339
                     /locus_tag="VGE92_02415"
     CDS             972..2339
                     /locus_tag="VGE92_02415"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEY1312696.1"
                     /translation="MSDGQSNSGREGNLEAPTRHALGWQEPQFYDAAALQKELERVFD
                     ICHGCRRCFNLCNSFPTLFDLIDGSKSGELDAVDKADFSKVVDQCYLCDMCYMTKCPY
                     VPPHPWNVDFPHLMLRAKAVKNRSGGTLWRDKVLSSTDMVGKIAGIPVVAEVVNAVNR
                     STIGRKILDGVLGVHSQAPVPQYHSNTLRKRLAKRRKPVPDAHSAGATRGKVALFATC
                     YANRNEPQIGLDLASVFEHNGIEVALLAQENCCGMPKLELGDLPAVAALKARNIPQMI
                     TMIDRGYDIVAPIPSCVLMFKQELPLMFPDDLEVKKVRGHVFDPFEYLALRHKFGLLR
                     TDFTRSLGKVSYHVACHLRVQNIGLKTRDVLQLIPGTSVEPIERCSGHNGTYGVKREF
                     RDISMKIAKPVIQRVESSGADFYASDCPMAGHQIESGLAEHGPPPTHPLTLLRMAYGL
                     GEDPP"
     gene            2336..2920
                     /locus_tag="VGE92_02420"
     CDS             2336..2920
                     /locus_tag="VGE92_02420"
                     /inference="COORDINATES: protein motif:HMM:NF023432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HEY1312697.1"
                     /translation="MKKLARGDLLSLEQYASDRARLRSEVIAHKQLRNVSVGPNMTWC
                     FEDSTTIRYQIQEMLRAERIFEIEGIQSELDAYNPLIPDGSNWKVTLLLEFPDPEIRR
                     VALEKLIGVEDRCWVRVSEMERVFAIADEDMERENEAKTSAVHFLRFELSPSMVQAMK
                     SDASLSIGVDHDAYRHLVSPLSRPVRDALNRDLK"
     gene            2954..>4602
                     /locus_tag="VGE92_02425"
     CDS             2954..>4602
                     /locus_tag="VGE92_02425"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY1312698.1"
                     /translation="MIAGTETAQRTPPNGSEGANRVWLDAVSSGICSPAVFYRAMQDQ
                     HQGNPEENWEVLSLLDQYYRRGKIKADLFQTLKAHLESEALGGQGTAPARPKPAAAPP
                     TITRPTPVPAAPPPVAPAALATMPFAAVPAAAEHDETPQKAATPRARAVREVAGGTLL
                     RGRYRLGSVVGQGGMGTVFEATDEYRLDLPTTTQRVAIKVLRPAISQREDLLYEVQRG
                     FQHLQLLSHPNIVRVHEFDRDADVAFFSMEFLSGAPLSRVLSSRNAAGLPRPYALTVM
                     RDVGAALAYAHSMGLVHGDLTPHNVFITNNGELRVLDFAASHKLLREKWANDIELAQR
                     APVAAPGYASCQLLEGHNPDARDDIFALACISYMLLSGSHPFPNRTAVEAYAQHFKPR
                     RPPQLTGHQWKVLREGLRWERDRRPADVEDWLKRFGLVGAAPRLPSSTVLASTPPPPR
                     RTALMAGAVAATLILIAGGAYWALTHTDILGRLEADTASAPPPAETTSQEQPAVREEP
                     TPAPTLVAPPGPAVPQPRAALAAPAIAVSPSVKPSPAATPAP"
BASE COUNT          932 a         1445 c         1402 g          823 t
ORIGIN      
        1 gcgcgtagcc acgggcaccg gcgtcatagc ccgccgatgg gtaaccaaca tccggtgtgg
       61 cggccgaaat tgccgcggat gcaaccacga gaggtagcgt ggccagaccc actacggctc
      121 cgagcaaccc gtggccgaaa ccccatacgt gtgcatggcc gaagccgtgc cctcccgcca
      181 aggcgggcgc cacgggcgca caggcgatcg cggcaacagc cacccaccca cccatcgatc
      241 gattgaatct gcgcatggca agcccccgca acgactgaac ccacccgatg cgctccaccc
      301 tacgcgcgtt tgtttcgaaa cgcaacggta ccgtttcgtt atgctgcgct acaccattgc
      361 aggatcgcct gcgcgattga tgctgactat cgcgctgata gagagcgacg atttgactta
      421 gcgcagccct gaggtctaac ttcagattca ttaattcgga aggagtcaac cgtgaattta
      481 aaaggtagca aaacggaaaa gaatctgaag gacgcatttg ccggcgaatc gcaggcgaat
      541 cgacgttatt tgtatttcgc ggccaaggcc gacgtcgagg gcttcaatga tgtgtccgcc
      601 gtgttccgtt cgactgcgga aggcgaaacg ggccatgcgc atggacattt ggaatacctc
      661 gaggcggttg gcgacccggc gaccggattg ccgatcggtg cgacctcgct gaatctcaaa
      721 gcggcaatcg ccggcgaaac gcacgaatac acggacatgt atcccggtat ggcgaagagt
      781 gcgcgcgacg aaggcttcgg cgaaatcgcc gactggttcg agaccttggc caaggcagaa
      841 cgatcgcacg ccaatcgctt tcaaaaggcg ttggacaacc tctagtcggg cagcaccgcg
      901 ctcgcggcgg caaagcactg cattcgcggg ctcctcacgg agcccgcttc catttttggt
      961 cgggagatag cgtgtccgac ggacaatcca actcaggtcg cgaaggaaat ctggaagcgc
     1021 ccacccggca cgccttggga tggcaagaac cgcagttcta cgacgccgcg gctctgcaga
     1081 aggaactcga gcgcgtattc gatatctgcc atggttgccg ccgttgcttc aatttgtgca
     1141 actcctttcc gactttgttc gatttgatcg acgggtcgaa gagcggcgag ctcgacgcgg
     1201 tggacaaggc ggatttcagc aaggtagtcg atcagtgcta cctctgcgat atgtgctaca
     1261 tgaccaagtg cccatacgtg ccgccgcatc cttggaacgt cgattttccg catctcatgc
     1321 tgcgcgccaa agccgtcaag aatcgcagcg gcggcacgct gtggcgcgac aaggttttga
     1381 gttccaccga catggtcggc aagattgccg gcataccggt ggttgccgaa gtcgtgaatg
     1441 ccgtcaatcg cagcacgatc gggcgcaaaa ttctcgatgg cgtactcggc gtgcacagcc
     1501 aagctcccgt gccgcaatac cactccaata ccttgcgcaa gcggctggcc aagcgccgta
     1561 agcccgttcc cgacgcgcac agcgcgggag cgacccgcgg caaggtggcg ttatttgcaa
     1621 cctgctacgc aaatcgcaac gagccgcaaa tcggtcttga cctggcgagc gtgttcgagc
     1681 acaacggcat cgaagtggcg ctgctcgcgc aggagaattg ctgcggcatg cccaagctgg
     1741 agttgggtga tttgccagcg gttgccgctc ttaaggccag gaacatcccg cagatgatta
     1801 ccatgatcga ccgcggctac gacatcgtgg cgccgattcc ctcctgcgtc ctgatgttca
     1861 agcaagaact gccgttgatg tttcccgacg accttgaagt caagaaggtg cgcgggcacg
     1921 tcttcgaccc gttcgaatat ttggctctgc gccacaagtt cggattactg cgcaccgatt
     1981 tcacgcgttc actcggcaaa gtgtcctacc acgtggcctg ccacctgcgc gtgcaaaata
     2041 tcggattgaa gacccgcgat gtgttgcagc tgattcccgg aacatcggtg gaaccgatcg
     2101 agcgttgctc gggtcataac ggtacctacg gcgtcaaacg agagtttcgc gacatttcca
     2161 tgaaaatagc caaacccgtc attcagcgcg tggagtcgag cggcgctgac ttctacgcga
     2221 gcgattgtcc gatggcgggg catcaaatcg aaagcggcct tgcagagcat ggaccgcccc
     2281 cgacgcatcc cctcacgctc ctcagaatgg cctacgggtt gggggaagac ccgccgtgaa
     2341 gaaactggca cggggcgacc tgttgagctt ggagcaatac gcctcggatc gagcgcggct
     2401 gcgcagcgag gtcattgccc acaagcagct gcgcaacgtc tcggtgggac ccaacatgac
     2461 gtggtgtttc gaggactcga ccaccatacg ctatcaaatc caggagatgc tgcgcgccga
     2521 gcgcatcttc gagatcgagg gcattcaaag cgaactcgac gcgtacaatc cgctgattcc
     2581 ggacggcagc aactggaaag tcaccttgct gttggaattt ccggaccccg agatccgccg
     2641 tgtcgcgctc gaaaaattga ttggcgtgga agaccgctgc tgggttcgtg tcagcgaaat
     2701 ggaacgcgtt tttgcaatcg cggatgaaga catggagcgt gagaacgagg ccaagacgtc
     2761 ggcagtgcat tttttgcgat ttgagctctc cccatccatg gtccaagcga tgaaaagcga
     2821 tgcgtccttg agcatcggcg tcgatcacga cgcctatcgg cacctcgtca gcccattgtc
     2881 ccgtccggtt cgggacgcat tgaatcgcga cctcaagtag ggaacactac agctagcccg
     2941 cgaggaggtc gatatgatcg ccggaacaga gacagcccaa cgaacgccgc cgaacggcag
     3001 cgagggcgcg aatcgcgtgt ggctggatgc cgtctccagc ggaatttgca gccccgcggt
     3061 attttatcgt gcgatgcagg atcagcatca gggaaacccc gaagagaatt gggaagtctt
     3121 gtcgctgctc gaccaatatt accggcgcgg gaagatcaaa gccgacctgt tccagacgct
     3181 gaaagctcat ctggagagcg aggcgctcgg cgggcaaggc acggcgccgg cccggccgaa
     3241 gccggccgcg gcaccgccga cgattacgcg cccgacgccg gtgcccgccg cgccgccccc
     3301 cgttgccccc gcggcgcttg cgacgatgcc gttcgccgcc gtgccggccg ccgccgagca
     3361 tgacgaaaca ccgcaaaaag cagccactcc acgtgcgcgc gcggtccgag aggtagcggg
     3421 cggtacgctg ctgcgcggac gctatcgact cggctcggtc gtcggtcaag gcggcatggg
     3481 tacggtgttc gaggcgaccg acgaataccg cttggatctg cccacaacga cgcagcgcgt
     3541 agcgatcaag gtgctgcggc cggcgatcag ccagcgcgaa gacctgctgt acgaagtgca
     3601 gcgcggcttt cagcatctgc agctcctgtc tcatccgaac atcgttcgcg tgcatgagtt
     3661 cgatcgcgat gccgatgtcg ctttcttcag catggaattc ttgagtggcg cgccattgag
     3721 ccgcgtgttg agctcgagaa atgcggccgg tttgccgcga ccctacgcgc tcacggtgat
     3781 gcgcgatgtc ggcgccgcgc tcgcgtacgc gcactcgatg ggactcgtgc atggtgatct
     3841 gacgccacac aatgtattta ttacaaacaa tggcgaactg cgcgtgttgg atttcgccgc
     3901 ttctcacaaa ctgctgcgcg agaaatgggc gaacgacatc gagctcgcgc aacgcgcgcc
     3961 ggttgcggcc ccgggatacg cgagttgcca gctgctcgaa ggtcacaatc ccgatgcccg
     4021 cgacgatatt tttgcgctcg cgtgtatttc ctacatgctg ttgtcgggga gccatccgtt
     4081 cccgaaccgc accgccgtcg aagcatatgc gcaacacttc aagccgcgcc gccccccgca
     4141 actcaccggt caccaatgga aagtgctgcg tgaagggcta cgctgggagc gcgatcggcg
     4201 gccggcggac gtcgaggact ggttgaagcg ctttggcctg gtgggcgccg cgccgcgcct
     4261 gccctcctcg accgtgctgg cgagtacacc gccgccgccg cggcgcacgg cgctcatggc
     4321 cggcgccgtt gccgcgacgc tgatcctcat cgccggcggc gcctattggg cgctgaccca
     4381 caccgacatc ctcggacggc ttgaagcgga cacggcgagc gcgccgccgc cggcggaaac
     4441 gaccagccag gagcagcctg cggttcgcga ggagccgacc ccggcgccga cgctggtagc
     4501 gcctccagga cccgccgtgc cgcagccgcg ggcagccctc gcggcgcccg caatcgccgt
     4561 ctctccgtcc gtcaagccct cccccgccgc aacgcccgct cc
//
LOCUS       DASWZT010000093         2637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_92618, whole genome shotgun sequence.
ACCESSION   DASWZT010000093 DASWZT010000000
VERSION     DASWZT010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92618"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..2467
                     /locus_tag="VGE92_02430"
     CDS             320..2467
                     /locus_tag="VGE92_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEY1312699.1"
                     /translation="MTGLVQRRSGLVCDKILRRHQDRLAIVYVRQSTVQQVERHQEST
                     KLQYALADRAAQFGWMQEQIIVIDEDLGRSGASIEGRLGFQRLVAEVGLGKVGLVLGV
                     EMSRLARSCRDWHQLLEICALFDTLIADADGVYDPANYNDRLLLGLKGTMSEAELHIL
                     KARMLAGRKAKARRGELGKAVPMGYVRCPSGEVAFDPDEQAQATIRLVFDLFDRFKTV
                     GKVMIYLAENEIRMPVRLRSGPKKGDLEWRRVNRASLLNLFANPIYAGVYAYGVRAVE
                     KRRQKPGRPGTGRKAPRPADAEVFLHDHLPAYISFERYERNQAQLRSNRADWGGAPRA
                     GTALLSGLLVCGCCGLRMLAQYNNNGHTARYTCQAMKSSYGDPYCQSLKAQPLDALIA
                     RLVLNAVAPAAIEASLALAQNLEAERAALDRHWRQRLERAGYEVERAHRQYAAVEPEN
                     RLVARTLERHWEAALTEQARLEAEYERHKRTRRHAPSVVELAAIRELTHDLPALWQAE
                     TTMQLERQTIVRLLLERVVVEVVAGTEKVRVECHWHGGSRTTHELTRPVARLAMLSTY
                     AALTARAAELRHEGCECAKIAEILNAEGWRPAKRRDTFNAQMVHHLLLKSGAETLKYR
                     RRPSHIERLENEWTIRELAQEIGMPQPTLYTWVQKGRLSCRNIGGGSKRAKLVRADAE
                     TIAALKAIRATPPHWRRPPPGLANSAATPATES"
BASE COUNT          524 a          806 c          850 g          457 t
ORIGIN      
        1 gcatattgct gagaagaagg tgtgcgcgca tcgatcatag cgggtgtgga tgcgccgcca
       61 gtccttgagc ttgccgaaca tattttcgat atcccattca gaataccgct cataatttga
      121 cggaaagcga cgaatcgccg agaatcagct tcgaagcgct atccgcactg gcgcgttggc
      181 ttgtatcccg cgatgagcga tctgccggcg tcgatttgga acatgttgcc cccggaacgg
      241 cggggacgca tagcgacgct cctgggacaa atggccttgc atcggatgcg cgacgcgaca
      301 gttgccccgg aggcgcccaa tgacgggcct tgtccaacgt cgcagcggct tggtctgcga
      361 caagattctg cggcgccacc aagaccggct ggcgattgtc tatgtccgcc aatccacggt
      421 tcaacaggtt gagcgtcatc aggagtcgac caagctgcaa tacgcgctcg cggatcgcgc
      481 ggcgcaattt ggctggatgc aggagcaaat catcgtcatc gacgaggatc tcgggcgctc
      541 cggcgccagc atcgaggggc gcttgggttt tcagcgattg gtcgctgagg tcggccttgg
      601 caaagttggg ctcgtgctcg gcgtcgagat gtctcggctg gcgcgatcct gcagggattg
      661 gcatcagctt ctggaaatct gcgcgctgtt cgacactctg atcgccgacg ccgacggggt
      721 gtatgatcca gccaattaca acgatcgtct gctgctgggg ctcaaaggga cgatgtcgga
      781 agctgagctg catatcctca aggcaaggat gctcgcgggc cggaaagcca aggcgcggcg
      841 gggcgaactg ggcaaggccg tgccgatggg ctatgtgcgc tgcccctctg gcgaggtggc
      901 gttcgatcct gacgagcagg cgcaggcgac gatccgcctg gtcttcgact tgttcgatcg
      961 cttcaagacg gtcggcaagg tgatgatcta tctggccgaa aacgaaatcc gcatgccggt
     1021 gcgcttgcga agcggaccga aaaaaggcga tctcgaatgg cgccgcgtca atcgcgcgtc
     1081 tttgctcaac ctcttcgcca atcccatcta tgcaggcgtc tacgcctatg gcgtgcgagc
     1141 ggtcgagaag cgccggcaga agcccgggcg accgggaacc gggcgcaaag cgccgcgccc
     1201 cgcggacgcc gaggtctttt tgcacgatca cttgccggcc tacatcagct ttgagcgcta
     1261 tgaacgcaac caagcgcaat tgcgatccaa cagagcagat tggggcggag cgccgcgggc
     1321 gggaaccgct cttctttccg gcctgctcgt ttgcggctgc tgcggcctgc gtatgctcgc
     1381 gcaatacaac aacaacggcc atacggcgcg ctacacctgc caggcgatga agagcagcta
     1441 cggcgatccc tactgccaat cgttgaaggc tcagccattg gatgcgttga ttgcgcgtct
     1501 cgtgctcaac gcggtggcgc cggctgcgat cgaggcgagc ttggcgctcg cccaaaatct
     1561 cgaggccgag cgcgcggcgc tcgatcgcca ttggcgccag aggctggagc gcgcaggcta
     1621 cgaggtcgaa cgcgcgcacc gacagtatgc cgccgtggag ccggaaaacc ggctggtggc
     1681 gcgcacgctc gaacgccact gggaagcggc gctaacagaa caggcgcgcc tggaggccga
     1741 atacgaacgc cataagcgaa cgcggcggca tgcgccgagc gtggtcgaac tcgcggcgat
     1801 ccgcgaattg acgcatgact tgcctgcgct ctggcaagcc gaaaccacga tgcaattgga
     1861 gcggcagacc attgtgcgct tgctgctcga acgggttgtg gtcgaggtcg tcgcgggcac
     1921 ggaaaaggtc cgcgtcgaat gccattggca tggcgggagc cgcacaacgc acgagctgac
     1981 gcgtcccgtc gcgcgtcttg cgatgctcag cacttatgca gcgctgacgg cccgcgccgc
     2041 agagctgcgc cacgaggggt gcgaatgcgc gaagatcgcc gagatcctga acgccgaagg
     2101 atggcgcccg gccaagcgtc gcgacacctt caatgcgcaa atggttcatc atctcctgct
     2161 caaatcggga gccgagacgc tcaaatatcg ccgtcggccg tcgcacatcg agcgcttgga
     2221 gaacgaatgg acgatccgcg aactcgcgca agagatcgga atgccgcagc cgaccctcta
     2281 cacctgggtc cagaagggac gcctatcctg tcgaaacatt ggcggcggct cgaaacgtgc
     2341 caagctggtg cgcgccgacg ccgagacgat cgccgcgttg aaggcgatcc gcgcgacgcc
     2401 gccgcattgg cgccgcccgc cgccggggct cgccaacagc gccgccaccc ctgccacaga
     2461 atcttgagag tcgccatgcc tggtcgatat cgctatcgcc gcgatcgcga tacagctggc
     2521 cgaaactcac aagctcctcg tcgagcgatg tggcgagaac ggaatattcg tagacgtcga
     2581 cgcctgcgcc cacatcgacg aaagacaatg tttgctggcc tgacgtctca ttcgaag
//
LOCUS       DASWZT010000094        10622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_53892, whole genome shotgun sequence.
ACCESSION   DASWZT010000094 DASWZT010000000
VERSION     DASWZT010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10622
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53892"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VGE92_02435"
     CDS             <1..268
                     /locus_tag="VGE92_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY1312700.1"
                     /translation="YKITLPLSRNGILAGSMLVFIPAVGEFVIPALLGGPNALMIGRV
                     LWDEFFSNRAWPVASAVAILLLILLVPFMMWYQSVQARESAGAR"
     gene            265..1092
                     /locus_tag="VGE92_02440"
     CDS             265..1092
                     /locus_tag="VGE92_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY1312701.1"
                     /translation="MNYKSSYFILTCLGFGFAFLYIPILSMVVFSFNASKLVTVWDTA
                     NSPTLKWYYSLLNNEEILDAAWISLKVAVINASGSVILGTLAGFGLARFSQFRGRFSL
                     NAMTTAPLVMPEVITGLSLLLLFVALEQLIGWPAGRGITTMTIGHMTFSMAYVTVIVN
                     SRLASFDTALEEAAMDLGARPLKVFFVITLPIIFPAILSGWLLAFTLSWDDLVISSFT
                     SGPGSSTLPMVVFSKVRLGVSPEINVLATITIAVVTMMIVGVSLWMARVAKLDKAAA"
     gene            1124..1945
                     /locus_tag="VGE92_02445"
     CDS             1124..1945
                     /locus_tag="VGE92_02445"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY1312702.1"
                     /translation="MNNQLDPLAHAKDTLLDLAIRFGPKVLVAILIISVGFFVAGWVG
                     RAAARGLRRFDLEPPVRQLLTRVARVLVVGLFVIMALQNLGVELLPLIAGLGVAGAGI
                     ALATQGVLSNMVAGLTIIFTKPYRVGEYIAIAGVEGQVESITLFNTALTHPDRSRIIV
                     PNRKVVGEILHNYGRIRQSEIRVGVAYESDLALALATIRDLVHANPRVLAEPTPVIQV
                     MTLADSAVQIAVKPWVAVGDYIAVAGELNLSLVEGLRRHGIGIPYPQREVRLIGG"
     gene            1966..3093
                     /locus_tag="VGE92_02450"
     CDS             1966..3093
                     /locus_tag="VGE92_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY1312703.1"
                     /translation="MRDFALEVFFSRWEFAAKYNMAASDSESMTLAQLLALADPADRA
                     EFDGVSLGYTQTFGAPALRAEIASTYESITAGQLLCCAGAEEAIYIAMQVLLRREDHA
                     IVITPNYQAAETVPLGICEVTGVALDIDRRWDLDIDRLRAALKPNTKLLSINFPNNPT
                     GKIISRATFEAIVEICSARGIWLFSDEVYRFMERDPAQRLPQVADVYERGVSLNVMSK
                     AYGLAGLRIGWLACKDPAMLVRFERCKHFLSICNAAPSEVLARIALKAKDQLLQRNRR
                     IVRENLAVLNGFFSDYAHLFDWREPDGGCIGFIRYLGAEGVEEFTRRLLEESGVLFLP
                     SSVYRSELGPVPENCLRVGFGRAHVPAGVAALRGWLEENEK"
     gene            complement(3107..7009)
                     /locus_tag="VGE92_02455"
     CDS             complement(3107..7009)
                     /locus_tag="VGE92_02455"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HEY1312704.1"
                     /translation="MNAVANLPELGRRREEREPESRLREIPYNYTSLADREIVIRLLG
                     GAAWAILDELRAERRTGRSARMLYEVLGDIWVVDRNPYLQDDLLDNPKRRRQLIEALH
                     HRLNELDKRRDTTDAARDSKVGQLLRAASEAVASFERRFAELEQMRRKARRLFARHSR
                     ADNIRFDAFSRVAHVTDATDWRVELPFVVLAPDTEAETAALVRACIELKLTVIPRGGG
                     TGYTGGAVPLTPWSAVINTEKLETLGAVETAEWPGVPAGAASIFSAAGVVTKRVSEAA
                     ERAGFVFAVDPTSADASCVGGNIAMNAGGKKAVLWGTAVDNLAWWRMVDTHGNWLEVT
                     RLNHNLGKIHQVALACFELLWKDGRAAPEHAKILRSERLEIQGAAFRKTGLGKDVTDK
                     FLGGLPGVQKEGCDGLITAARWIVHRMPKHIRTVCLEFFGQARDAIPSIVEITQYLES
                     TAKIEGAVLAGLEHLDERYLRAVGYSTKSKRGALPKMVLLGDIVGADDAKVAAATSHV
                     VRLANARAGEGFVAVSPEARRKFWLDRARTAAIAKHTNAFKINEDVVIPLDRLGEYTD
                     AIERINIELSIGNKLELLDGLDDYFSGEIKPGKTADVDLGRRTEVRELLGRVRARWQY
                     LLGAMDTPVGAARTDLEALEIEPPAEDTGVIFDRLQDRSIRVSWKAEVRQVLTRIFAG
                     AAFSQVLAECEAVHKRVLQGRVFVALHMHAGDGNVHTNIPVNSDNYAMLHTANRAVAR
                     IMRIARNLNGVISGEHGIGITKLEFLTEAELASFRAYKQRIDPEGRFNKHKLMPGADL
                     RRAYTPSFGLLGHESLIMQQSDISTISDAIKDCLRCGKCKPVCATHVPRANLLYSPRD
                     KILATSLLIEAFLYEEQTRRGISLQHWDELSDVADHCTVCHKCEAPCPVDIDFGDVSM
                     AMRDLLRRMGKKRFNPGTAASMFFLNATNPQTIKLTRKLLIDWGYKGQRLANRALNTF
                     ARAQTKRPPATEGRAPIKEQVIHFVNKRMPGGLPRRTARALLDIEDRNYVPIIRDPHK
                     TNAESEAVFYFPGCGSERLFSQVGLATQAMLWHVGVQTVLPPGYLCCGYPQRGAGEFD
                     KAEKIITDNRVLFHRVANTLNYLDIKTVVVSCGTCFDQLQGYKFEDIFPGCKIIDIHE
                     YLMEKGVHLENVTGVRYMYHDPCHSPMKKHDPLLVVNTLMNSAQNGQIEKNERCCGEA
                     GTLAVTRPDISTQVRFRKEEEIRSAAEEIRAAGHRDAVKILTSCPSCLQGLKRYNDDT
                     DTDADYVVVEIAKNLLGPDWLPQYVQRANSGGIERVLV"
     gene            7149..7976
                     /locus_tag="VGE92_02460"
     CDS             7149..7976
                     /locus_tag="VGE92_02460"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEY1312705.1"
                     /translation="MRQLMELHLANDELTVPLLPEVAVRVVRAGSNKSTDARQLADII
                     NADPALTMYVLRIAASAAKRPASPIVTLPHAVAWLGLDEVANIAFTLALQGKMLDVKG
                     QQHKARRLWRHSLASALWARQLAHMLAEETGLCYLCGLLHNIGKVVTLGAVYELARQA
                     GSKLASGDFDRLIEIFHRHVGAHVVGAWELPAPVLTVTSEWESYAAAGPAKFERNVVN
                     VAHALADFTLQGSTHLARDLLMTHSAYRDLGLSAADGEPLFDSAVAINAELDRYLAP"
     gene            8013..9230
                     /locus_tag="VGE92_02465"
     CDS             8013..9230
                     /locus_tag="VGE92_02465"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEY1312706.1"
                     /translation="MGELSMWAALDYLAGMKQNTQVNHPPAVPLPADNRPLVAPIYQS
                     VKFSFDDTAETLRYLRGERDGFFYSRSANPTLLQLQLLLAQLQGRDDCLLTGSGVATI
                     AASLLALCKQGDHVLAFVESYGPTRYIVQHLLAKFGVTHTLLSIEDLAGIERVLLGKP
                     TRLVVFESPTNPVTKIADIEHLTRHARRAGALTVLDNTFAGFHNHGAYDIDIYLHSLT
                     KYASGHGDVMGGAIIARAPLIAEMRKDIVSMGPTLDPHAAFLIQRGMRTYFLRYERQC
                     ANAMAVSEFLNSHPRVSSVHYPGLATHPQHQLARRQMRDFGTVVTLELRGGVEQGARF
                     AEALQYFSISASVGSAESLVMPPQLLGPNEYTPEQRAAALIGRGTVRLSIGLEDAADL
                     IEDLAQALAKAFI"
     gene            9304..10158
                     /locus_tag="VGE92_02470"
     CDS             9304..10158
                     /locus_tag="VGE92_02470"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEY1312707.1"
                     /translation="MGLESAVVLSALHPQVARDYLSESLAGPQTIAPLPRVCAQLAQL
                     TAQEATDAARLAKLIQSDPSLAGELMRVANSPALRPRSAIVSLQQAVSWLGVAEVRNI
                     AMAVMLRGEVFVAPGHEPESEDLWREASLAGLWAKEIARERRKQVESAFLAALMHRTG
                     AALALKILSRFELEKRTVMDARTFANLVVEFEPSFGRLLMDNWLLPKDVQAGASDWRN
                     YRESEHSDLAGTVHAAHLLATHTMHPQLLTEDVVIESPVFEKLGVFPDARQRMLAKRD
                     QLRDLAGM"
     gene            10160..>10622
                     /locus_tag="VGE92_02475"
     CDS             10160..>10622
                     /locus_tag="VGE92_02475"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1312708.1"
                     /translation="MAQESFDFLCVGSGPAGQKAAIQAAKAGFRAAIIEREAQVGGSC
                     LLSGTIPSKALREQALRYRRMQGSASSLAVELRGDAPLSALLHGVDIVIAAQDRYLQA
                     QLARNHVELIRGHGVFRDANCIEVQRLDGSRIILRAARVLLATGSRPRHVPA"
BASE COUNT         1814 a         3279 c         3382 g         2147 t
ORIGIN      
        1 ctacaagatc accttgccct tgtcgcgtaa cggcatcttg gcgggctcga tgctggtgtt
       61 cattcccgcg gtgggtgagt tcgtcatccc ggcattgctc ggtggtccga acgccctgat
      121 gatcggacgc gtcttgtggg atgagttctt ctcgaatcgt gcatggccgg tggcgagtgc
      181 ggtggcgata ctgctgttga ttctgctggt gcccttcatg atgtggtacc aaagtgtgca
      241 ggcgcgcgag tcggcgggtg cgcgatgaac tacaagagct cgtatttcat cttgacctgc
      301 ctcggattcg gattcgcctt cttgtacatc ccgatcctgt cgatggtggt gttctcattc
      361 aacgcctcga agctggtgac cgtctgggat acggccaact cgccgacgct caagtggtac
      421 tactcgctgc tcaacaacga ggaaatactc gatgcggcat ggatttctct caaagtcgcc
      481 gtcatcaacg cctccggctc agtgatcttg ggcaccttgg ccggcttcgg cctggcgcgc
      541 ttcagtcagt tccgcggccg cttctcactg aatgcgatga cgacggcgcc gctggtcatg
      601 ccggaggtca tcaccggcct gtcgctgctg ttgctgttcg tcgctctcga acagctgatc
      661 ggttggccgg ccggacgcgg tatcacgacc atgacgatcg ggcacatgac cttctcgatg
      721 gcctatgtca cggtgatcgt gaactcgcgg ctggcgagct tcgatacggc gctcgaggaa
      781 gccgccatgg atttgggtgc gcgcccgctc aaggtgtttt tcgtcattac gttgccgatc
      841 atttttcctg ccatcttatc gggatggctg cttgctttta cgctttcgtg ggatgatctg
      901 gtcatctcga gtttcacgtc gggtcccggc agcagtacgc tgcccatggt ggtgttctcc
      961 aaggtgcgtt tgggggtgag ccctgaaatc aatgtgctcg ccaccatcac gatcgcggtg
     1021 gtgaccatga tgatcgtcgg cgtgagtctg tggatggcgc gggtcgccaa gctcgataag
     1081 gcggcggcct agccggcgcc gctcatacag ttaagggaca ccgatgaaca atcaactcga
     1141 cccattggca cacgccaagg acacgttgct cgatcttgcg atccgcttcg ggccgaaggt
     1201 gctggtggcg atcctcatca tcagcgtcgg tttcttcgtc gcaggctggg ttggccgtgc
     1261 ggccgcgcgc gggttgcggc gcttcgacct cgagccgccg gtgcgccaac tcctgacgcg
     1321 ggtcgcgcgg gtgctggttg tcggtttgtt cgtcatcatg gcgctgcaga atctcggcgt
     1381 cgaattgctg ccgttgatcg ccgggctcgg cgtcgccggc gccggcatcg cgctcgccac
     1441 gcagggcgtg ctcagcaata tggtcgccgg cctcaccatc attttcacca agccgtatcg
     1501 cgtcggtgaa tacatcgcga tcgccggggt tgaagggcag gtcgagagca tcacgctgtt
     1561 caataccgcg ctgacgcatc ccgatcgttc gcggatcatc gtgcccaatc gcaaggtggt
     1621 cggcgaaatc cttcacaact acggccgtat ccgccaatcg gagattcgcg tcggcgtcgc
     1681 ctacgagtcg gaccttgcgc tcgcgctcgc caccattcgc gacctggtgc atgccaaccc
     1741 gcgcgtattg gcggaaccga cgcccgtgat tcaggtgatg acgctggccg attcggcggt
     1801 gcaaattgcc gtcaagccgt gggttgcggt cggcgattat atcgccgtcg ccggcgagct
     1861 caatttgtcg ctggtcgagg gactgcgccg gcacgggatc ggtattcctt acccgcagcg
     1921 tgaagtgcgt ctgatcggcg gctaacgctg cgcctgatcg atacgatgcg cgattttgca
     1981 ctcgaggttt ttttctctcg ctgggagttc gccgccaagt acaacatggc cgcttccgac
     2041 agcgagagca tgacgctggc gcagctgttg gcgctggcgg accccgcgga tcgcgccgag
     2101 ttcgacgggg tcagcttggg ttacacgcag accttcggtg caccggcgtt gcgtgccgag
     2161 attgccagca cctacgaatc gatcacggcc gggcaactgc tgtgttgcgc aggcgccgag
     2221 gaggcaatct acatcgcgat gcaagtgctg ctgcgtcgcg aagaccacgc catcgtcatc
     2281 acgcccaact accaggcggc cgaaaccgtg ccgctcggca tttgcgaggt gaccggcgtg
     2341 gcgctcgata tcgaccggcg atgggacctc gatatcgatc gcttgagggc ggcgctcaag
     2401 ccgaacacga agttgctctc catcaatttt ccgaataatc caaccggcaa gatcatttct
     2461 cgggcaacgt tcgaggcaat cgtcgaaatc tgcagcgcgc gcggcatctg gctgttcagc
     2521 gacgaggtgt atcggtttat ggaacgcgat ccggcgcaac gcctgccgca ggtggccgat
     2581 gtctatgagc gcggtgtttc cttgaacgtc atgtccaaag cctacggctt ggcgggactg
     2641 cggatcggct ggctggcgtg caaggatccc gcaatgctcg tgcgcttcga gcgctgcaag
     2701 catttcctgt cgatatgcaa tgcggcgcct tcggaggtgc tcgcgcgcat tgcgctcaag
     2761 gcgaaggatc agctgctgca gcgaaaccgg cgcatagtgc gcgaaaatct cgcggtcttg
     2821 aacgggtttt tcagcgacta tgcgcatttg ttcgactggc gcgagcccga cggcggctgc
     2881 ataggcttca tccgctatct gggggcggag ggggtcgagg agtttacgcg ccggctgctc
     2941 gaggagtcgg gcgtgctgtt tctgccgtcg agcgtctacc ggtcagagct cggtccggtg
     3001 ccggagaatt gtctgcgcgt ggggttcggg cgcgcgcacg ttccggcggg cgtcgcggcg
     3061 ctgcgtggct ggctcgagga gaacgagaaa tgagcgcatg cccgcctcac accaataccc
     3121 gttcgatgcc gccgctgttg gcgcgctgta cgtattgcgg gagccaatcc gggcccaata
     3181 agttcttggc gatctcgacc acgacgtagt cggcgtccgt gtcggtatcg tcgttgtagc
     3241 gcttcaggcc ctgcaaacac gaaggacacg aggtcaatat cttgacggcg tcgcggtgtc
     3301 cggcggcgcg aatttcctcg gcagcggaac gaatctcctc ttccttgcga aagcgcactt
     3361 gtgtcgaaat atcagggcgc gtgacggcga gcgtgccggc ctcgccgcag cagcgctcgt
     3421 tcttctcgat ctgcccgttt tgcgcgctgt tcatcagcgt gttcaccacc agcaacggat
     3481 cgtgcttctt catcggcgag tgacaggggt catgatacat atagcgcacg ccggtgacgt
     3541 tttccagatg cacccctttc tccatgagat actcgtgaat atcgatgatc ttgcagccgg
     3601 gaaagatatc ttcgaattta tacccctgca gctgatcgaa acaggtgccg cagctcacga
     3661 cgacggtttt gatgtccaga tagttcaacg tattggcgac gcggtggaac aacacgcggt
     3721 tgtcggtgat gattttttcc gccttgtcga attcgccggc gccgcgctgc ggatacccgc
     3781 agcacagata tccgggcggc aatacggttt gcacaccgac gtgccacaac atggcctggg
     3841 tcgcgagtcc gacttgcgag aacaaccgtt cggagccgca gcccggaaaa taaaacaccg
     3901 cctcggactc ggcgttcgtc ttgtgcgggt cgcggatgat cggcacgtag ttgcggtctt
     3961 cgatatcgag cagcgcgcgc gccgtcctgc gcggcaatcc tcccggcatg cgcttgttga
     4021 cgaaatggat gacctgctcc ttgatcggcg cccgtccctc cgtcgccggc ggccgcttgg
     4081 tctgcgccct ggcaaaggta ttgagtgcgc ggttcgccaa gcgctgtccc ttgtaacccc
     4141 agtcgatcag gagcttgcgc gtcagcttga tggtttgcgg attggtggcg ttcaagaaga
     4201 acatcgatgc cgccgtcccc gggttgaagc gtttcttgcc catgcggcgc agcagatcgc
     4261 gcatcgccat cgagacgtct ccgaagtcga tgtcgaccgg gcagggcgcc tcgcacttgt
     4321 ggcacaccgt gcaatgatcg gcgacatccg acagttcgtc ccaatgctgg agcgaaatcc
     4381 cgcggcgagt ctgttcctcg tagagaaacg cctcgatcag gagcgacgtt gccaagatct
     4441 tgtcccgagg tgagtacagc agattggcgc gcggcacatg ggtggcgcac accggcttgc
     4501 atttaccgca gcgcaagcag tctttgatgg catcggagat ggtgctgatg tccgattgct
     4561 gcatgatcaa cgactcgtgt cccagcaaac cgaagctcgg cgtgtaagcg cggcgcaggt
     4621 ccgcgcccgg catcagtttg tgcttgttga agcggccttc agggtcgatg cgttgcttgt
     4681 aggcgcggaa tgaggcgagt tccgcctcgg tcagaaactc cagcttggtg atgccgatgc
     4741 cgtgttcacc ggaaatcacg ccgttgagat tgcgggcgat tcgcatgatg cgcgccacgg
     4801 cgcggttggc ggtgtgcagc atcgcatagt tgtcggaatt caccggaatg ttggtgtgta
     4861 cgttgccgtc gccggcatgc atatgcagcg cgacgaagac acgtccttgc aacacgcgtt
     4921 tgtgcaccgc ctcgcactcg gcgagcacct ggctgaatgc cgcgccggcg aaaatgcgcg
     4981 tgaggacttg ccgcacttcg gctttccagc tcacccgaat gctgcggtcc tggaggcgat
     5041 cgaaaataac tcccgtatcc tcggccggcg gctcgatctc gagcgcctca agatcagtgc
     5101 gcgccgcgcc caccggggta tccatcgcgc cgagcaagta ctgccagcgc gcgcgcacgc
     5161 ggccgagcag ctcgcggact tcggtgcgac gcccgaggtc gacgtccgcg gtcttgcccg
     5221 gtttgatctc gccggagaaa tagtcgtcga gcccatcgag cagctcgagt ttgttgccga
     5281 tcgacagctc gatattgatc cgctcgatgg catcggtgta ttcgccgagg cgatcgagcg
     5341 gaatgaccac atcctcgttg atcttgaacg cgttggtgtg cttggcgatc gccgcggttc
     5401 gagcgcgatc cagccagaac ttgcggcgcg cctcgggact gacggcgacg aaaccctcgc
     5461 ccgcccgcgc gtttgccagg cgcacgacgt gcgaggtcgc ggcggcgacc ttggcatcgt
     5521 ccgcgccgac tatatcgccc agcagcacca ttttcggcaa cgcgccgcgc tttgacttgg
     5581 tgctgtagcc gacggcgcgc aggtagcgct catccagatg ttcaagaccg gcgagcacgg
     5641 cgccctcgat cttggcggtc gattcaaggt attgggtgat ctcgacaatg ctcggaatgg
     5701 catcgcgtgc ctggccgaaa aactccaggc acacggtgcg gatgtgtttc ggcatgcggt
     5761 gcacgatcca acgcgcggcc gtgatgagtc cgtcgcagcc ctccttctga acgccgggca
     5821 ggccgcccaa gaacttgtcg gttacgtcct tgccgagacc ggtcttgcgg aacgcggcac
     5881 cttggatttc gagccgttcg ctgcgcagta ttttcgcgtg ctcgggagct gcgcgcccgt
     5941 ccttccagag gagttcgaag caggcgagcg ccacctgatg gatcttgccc aaattgtggt
     6001 tcaggcgcgt gacctcgagc cagttgccgt gcgtgtcgac catgcgccac cacgcaagat
     6061 tatcgaccgc cgttccccac agcacggctt tcttgccgcc cgcgttcatg gcgatgttgc
     6121 cgcccacaca ggaagcgtcc gccgaggtgg gatcgacggc aaacacgaag ccggcgcgct
     6181 ccgccgcttc ggacacccgc ttggtaacca cgccggccgc cgagaagata ctcgcggcgc
     6241 cggccggaac gccgggccat tcggccgttt cgacggcgcc caaggtctcc agtttctcgg
     6301 tattgatcac cgccgaccag ggcgtcagcg gcaccgcgcc accggtgtag ccggtgccgc
     6361 cgccgcgcgg aatgacggtg agtttcagtt cgatgcaggc ccgcaccagg gcggcggttt
     6421 ccgcctcggt gtccggtgcc agcaccacaa acggcagttc gacccgccag tcggttgcgt
     6481 ccgtgacatg cgcaaccctc gagaatgcgt cgaagcgaat attgtccgcg cgggagtgcc
     6541 gggcaaacag acgacgcgcc ttgcggcgca tttgctcgag ttcggcgaag cgcctttcga
     6601 aactcgccac ggcttccgac gcggcgcgca gcagctgccc gaccttcgag tcacgtgccg
     6661 catccgtggt atcgcggcgc ttgtccaatt cgttcaggcg atggtgcagt gcttcgatga
     6721 gttgccgccg ccgcttgggg ttgtcgagca gatcgtcttg caagtagggg ttgcggtcga
     6781 ccacccagat atcgcccaag acctcgtaca gcatgcgcgc cgaccggccg gtgcgccgct
     6841 cggcgcgcag ctcatcgagt attgcccagg cagcgccgcc gagcagccgg atgacgattt
     6901 cgcgatccgc cagagaggtg tagttgtacg gaatctcccg cagccgcgat tccggctcgc
     6961 gctcttcgcg acggcgcccg agctcgggca aatttgcgac ggcgttcata ttgtggggat
     7021 tttacgcatg aatcgaccgc ttatgtgcgc ctcaagtcgt ggggctcgcg gccgttatgc
     7081 agactatgca gaacccgtcg agcaaaccgc ggagcggcac gccggtcgaa tcggagacct
     7141 gccaggccgt gcgtcagcta atggagctgc atttggccaa cgatgagctg accgttccgc
     7201 tattgccgga agttgccgtt cgggtcgtac gcgccggttc caacaagtcg accgacgcac
     7261 ggcagctcgc ggatatcatc aacgcggatc ccgcgctcac catgtacgtg ctgcgcatcg
     7321 cggcatcggc ggccaaacgg ccggcatcgc ccatcgtcac cttgccgcat gcggtcgcgt
     7381 ggttgggact cgatgaagtg gcgaacattg ccttcactct ggcgctgcag ggcaagatgc
     7441 tcgacgtcaa gggacaacag cacaaggccc ggcggctgtg gcgccactcg ctcgcgagcg
     7501 ctctgtgggc acggcagttg gcgcacatgc tcgccgaaga gaccggtctg tgctatctgt
     7561 gcgggttgct gcacaacatc ggtaaggtgg tgacgctcgg cgccgtctac gaacttgccc
     7621 ggcaagcggg cagcaaactc gccagcgggg actttgaccg tctgatcgag atcttccatc
     7681 gtcacgtcgg cgcgcatgtt gtcggcgcat gggagctgcc cgcgccggtg ctcacggtga
     7741 cctcagaatg ggagagctac gcggccgccg gcccggcgaa attcgaacgc aatgtcgtca
     7801 acgttgcaca cgccctcgcg gacttcacgt tgcagggatc gacgcacctc gcccgcgatc
     7861 tgctcatgac gcattcggcc tatcgcgacc tcggactgag cgcagccgac ggcgaaccct
     7921 tgttcgattc cgcggtcgcc atcaacgccg agttggatcg atacctcgct ccctgagcgc
     7981 cggtgccgca accgtgccaa gcggcggcgc cgatgggtga actttcaatg tgggccgcgc
     8041 tcgattatct tgcagggatg aaacaaaaca cgcaggtcaa ccatccgccc gccgtgccgc
     8101 tgcccgccga caatcgtcca ttggtcgcgc cgatctacca gtcggtcaag ttctctttcg
     8161 acgacaccgc tgagacgttg cgctacctgc gcggcgagcg cgatgggttc ttctactcgc
     8221 gatcggcgaa cccgacgctg ctgcaactgc agctgttgct cgcgcagttg cagggcagag
     8281 acgactgttt gctgacgggg tcgggcgttg caacgatcgc tgcgagcctg ctggccctat
     8341 gcaagcaagg tgaccacgtt cttgccttcg tcgagtcgta cggcccgacg cgctatatcg
     8401 tgcagcatct gctcgcgaag ttcggcgtca cgcatacgct gttgtcgatc gaagatctcg
     8461 cgggcatcga gcgcgtgttg ctcggcaaac cgacgcgcct ggtggtgttc gaaagcccga
     8521 ccaatccggt caccaagatc gccgatatcg aacacctgac gcggcacgcg cggcgcgccg
     8581 gggccctgac ggtgctcgac aatacattcg ccggatttca caaccacggc gcctatgaca
     8641 tcgatattta tctgcacagc ctgacgaagt acgcgtccgg ccatggcgat gtgatgggcg
     8701 gcgccatcat cgcgcgcgcg ccgctgatcg ccgagatgcg caaggacatc gtgtcgatgg
     8761 gcccgacgct cgatccgcac gccgcttttc tgattcagcg cggcatgcgc acgtattttt
     8821 tgcggtacga gcgtcagtgt gccaatgcga tggcggtgag cgaatttctc aacagccatc
     8881 cgcgcgtgag cagcgtccat tatccggggc tcgcgactca tccgcagcat cagctggcac
     8941 gccggcagat gcgcgatttc ggcaccgtgg tgactctcga gcttcgcggc ggtgtcgagc
     9001 agggcgctcg attcgctgag gcgctgcagt atttttcgat ctcggccagc gtcggctcgg
     9061 cggagtcgtt ggtgatgccg ccgcagctgc ttgggcccaa cgagtacacg ccggagcagc
     9121 gtgccgccgc tctgatcggc cgcggcaccg ttcgcctgtc aatcggcctc gaagatgcgg
     9181 cggatctgat cgaggatttg gcccaagccc tggccaaggc attcatttaa cttaacatat
     9241 tcccctaaag catcctcgcc tcgtgccgat ctaacgggtt gacgtgcaaa cgaggggtag
     9301 tccgtgggtc tcgaatcggc tgtagttctt tccgccttgc atccccaagt ggcacgcgac
     9361 tatttgagcg aatcgctggc cggcccgcaa accatcgcac ccttgccgcg ggtctgcgcg
     9421 cagctcgcgc agctcacggc ccaggaggcg accgatgccg cccgcttggc aaaattgatc
     9481 caatccgatc cgtcattggc cggcgaactg atgcgggtcg ccaattctcc ggcgttacgg
     9541 ccgcgaagcg ccatcgtctc attgcagcag gcagtgtcct ggctcggggt tgcggaagtg
     9601 cgcaacattg cgatggcggt gatgctgcgc ggcgaagtat tcgtcgcccc cggacacgaa
     9661 cccgagtccg aggatttgtg gcgcgaggct tcgttggcgg gcttgtgggc aaaggaaatc
     9721 gcccgcgaac gccgcaagca ggttgaaagc gcgtttctcg ccgccttgat gcatcgaacc
     9781 ggggcggcgc tcgcgctcaa gattctgtcg cgcttcgaat tggagaagcg cacggtcatg
     9841 gacgcgcgca ctttcgccaa cctcgtggtc gaattcgagc cgtccttcgg ccgcctgttg
     9901 atggacaact ggctgctgcc gaaagacgtg caagccggcg ccagcgactg gcgcaactac
     9961 cgcgaatcgg agcacagcga tcttgcaggg accgtgcacg cggcgcacct cttggccact
    10021 cataccatgc acccgcaatt gctgaccgaa gatgtggtga tcgagagccc ggtattcgaa
    10081 aagctgggcg tgttcccgga cgcgcgtcag cgcatgctgg ccaaacgcga tcaactgcgc
    10141 gacctggcag gcatgtaacg tggcgcagga gtcgttcgat tttctgtgcg tgggatcggg
    10201 tcccgccggc cagaaagcag cgattcaggc ggccaaggcg gggtttcgcg ccgccatcat
    10261 cgaacgcgag gcgcaagtcg gcggctcgtg cctgctgtcc ggaacgattc cgagcaaggc
    10321 gctgcgcgag caggcgctgc gctatcgacg catgcagggc agcgcatcct ccttggcggt
    10381 ggagttgcgc ggcgatgcgc cgttgtcggc gttgctgcat ggcgtcgata tcgtgatcgc
    10441 cgctcaggac cgctacttgc aggcgcaact cgcgcgcaac catgtcgaac tcatccgcgg
    10501 ccacggggtg tttagggacg cgaactgcat cgaggtgcag cgcttggacg ggtcgcggat
    10561 tatcctgcgt gctgcgcgcg tactgctggc gaccggatcg cggccgcggc atgtgccggc
    10621 gg
//
LOCUS       DASWZT010000095        11667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_78092, whole genome shotgun sequence.
ACCESSION   DASWZT010000095 DASWZT010000000
VERSION     DASWZT010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11667
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78092"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VGE92_02480"
     CDS             <1..949
                     /locus_tag="VGE92_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312709.1"
                     /translation="LAARAAPNANGVGSAERNRGPAPIRSTPITLLARRNVPLWSALT
                     DQSDPPHLSSRALAVAEFIREQGASFFDEIAEHVGMLPVEVEGALAELVALGVVNSDS
                     FAGLRVLLLPSGRRSKSMSYTTRNKRRLALFGMADAGRWALVRRPATATAARRDEAVE
                     QIVRTLLRRWGVVFWKLLAREAGWLPPWRDILQCCRRLEARGEIRGGRFVAGFSGEQY
                     ATPEAVGLLRDLRRKPLTQGYLSLSAADPLNLIGIITPGARLASLAGNRLLYRDGLPI
                     ATYAADEVHYMEELPEKERWEVQTALLRRHTPLALDDLA"
     gene            960..2495
                     /locus_tag="VGE92_02485"
     CDS             960..2495
                     /locus_tag="VGE92_02485"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEY1312710.1"
                     /translation="MDPTRRRQGGRGRRLDGGRVAQLPWSEVDNPYPPMEVLSADQLE
                     AIHLTSLRILEDLGMEVMSARALAVLKTAGAQVDAAARIVRLDRALVERALDTAPRSF
                     VLTPRNPAKRVTLGGSRINFGLVAGPPNVHDCERGRRSGNYRDYCDFIRLAQYFSAIH
                     LIGNQVCAPIELPANSRHLDTYRANLGFSDQVYHCTAIGAGRALDGIRMMAIARGLSL
                     DEIAASPGVMAIISVNSPRRFDEAMTEGLMAMAEHGQAVVVTPFTLMGAMAPVSLAAA
                     LAQQNAEALFGVVLAQLIRPGTPVMYGAFTSNVDMRSGAPAFGTPENAKANIASGQLA
                     RRYRLPYRTSNASASNAADAQGAYETQMSLWGAILGHGNLVYHAAGWQEGGLTASFEK
                     LVLDVEMLQMMIEFLKPIVVDERELGFDAIKGVAPGGHFFGEAHTMERYEHAFYRPLL
                     SNWQNYENWQLAGAKDATQRATQTWKQALHEYEQPAMDAAIGEALDAYVARRRSEIGS
                     NDP"
     gene            2521..3678
                     /locus_tag="VGE92_02490"
     CDS             2521..3678
                     /locus_tag="VGE92_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY1312711.1"
                     /translation="MFEPFPKNYIWNLQTNLALICGGNHGEIDEACRPLRDAAARGAD
                     AGTALLFDSWIAVADQVARNAEADEKAGFRLSAGTKFGRASGYYLSAERMQSRDYAPR
                     WVAYERGLALYRKHVALRGLHVDFVDIPYGDASYPAIFVHDGSATPRPALISVNGLDS
                     MKEQVNMGGHGQSNLERGINTLFVDQPGTGEALRKRKLTAVYDSERWATPALEYLLTR
                     SDVIPNKVGIFGLSLGGYYAPRAAANEPRFALCAVMGANHLWGDLQRRRLAREGENPV
                     PHYWDHVMWVWGKKSLDEFMAYVPQITLDGQIERMKMPFLIMHGAGDRQIPVSDAHRS
                     YEQAVNSRARELRIFTKADFEVEHCGADNGCGMRDYAADWCAQTFAKIPPP"
     gene            3678..4964
                     /locus_tag="VGE92_02495"
     CDS             3678..4964
                     /locus_tag="VGE92_02495"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY1312712.1"
                     /translation="MRRDSQRLIALTLAAVALAACHSLACAAEAVSAMPVPVPEPSPQ
                     AIRLYQSGNVLWVVATLWGFMVPALIFFSGLSARLRDAARRRASRWYASLLIYLVLLA
                     SLLFLANLPLDYYSDFLRPHQYGLSSQAFAKWLQDEILNLGLGLIGAALVLWIPYLLL
                     KRARRRWWLYTWFASIPILIFLNFIGPIWIAPLFNDFGPMHDKALETRILQLADRAGI
                     DGAAVYEVNKSVDSKQLNAYVAGIGSTKRIVLWDTTLKELNPDEVLMVMSHEMGHYAL
                     GHVWKGLIYESLVLLVGFWCVHIGVEAIIRRYGARTSVHEPGDFASLPLFMLIFSAVG
                     FVLTPPLLAVSRHYEHEADRFGLEMTHENRSCALVFVKFIEHDLAYPLPSPLVMFLRA
                     SHPSAGERINFCNTYHPWLQGQEGRYTHYFKPMPAD"
     gene            complement(5053..6642)
                     /locus_tag="VGE92_02500"
     CDS             complement(5053..6642)
                     /locus_tag="VGE92_02500"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY1312713.1"
                     /translation="MSLSLKSPEQVSGFDATHAQTVDLRTCAAPRASSAALEQHLPFA
                     VDIGRVLRDRYVIEQRLGSGGRGTVYKALDRYRSDLPETHQYVAIKFLNDTGGCESEQ
                     LAALRREFYCTQMLSHRNVVKVHELDRDAELHFYTMELLEGELLSSVMDRLKGAPMAR
                     PYAWHIIREIGAGLAHAHARQVIHGDLKPHNIMITPCGDVRILDFGSSSFRTAQVAAD
                     AMHPGNTYPPLTPAYASCELLAGRAADPRDDLFAFACLSYELIAGSHPFQRRPATVAR
                     DHGIIASRPRGLKRQQWKALACALSWCRAARPSSTRAWLDDLLGERPARSVPAPRLGA
                     RTAALGVAIMFTAAAWVALAHWATGLSAGSAIAPLAAAIVQPPAKPVADIAAASLETS
                     NPPAPLTAGAVANAKPPAAAAALPIVVSRKNYRVRAGENFAEVRVHRTSLMPGDSSFV
                     WWTEAASAKPGVDYVDQEKAIQTFPRGGEWTSFFIKLVPKELRTQPKMFYIDVGKAGH
                     GASSVPITRAAIWLPMTRGRS"
     gene            complement(6785..7084)
                     /locus_tag="VGE92_02505"
     CDS             complement(6785..7084)
                     /locus_tag="VGE92_02505"
                     /inference="COORDINATES: protein motif:HMM:NF025107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY1312714.1"
                     /translation="MDRIHLDAYVLDVLMPDLVGHDRRPAAFIVYLYLLRLAAKSRHD
                     TVAVSLQTVATKTGLAKSTVQAAIRHLRRRRLLDPAVISTVDRPARRVLRPWLRT"
     gene            7168..7533
                     /locus_tag="VGE92_02510"
     CDS             7168..7533
                     /locus_tag="VGE92_02510"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY1312715.1"
                     /translation="MIKQLKFVSIPTRDQARAVKFYTEQLGFEVATDQPFDDRQRWIE
                     LRIANAATRVVLFTPPGQEDRVGTFFNGSFECDDVEATYRQLTQRGGVFVREPEKQPW
                     GTMAVFRDPDGNQFVLSSG"
     gene            complement(7530..8990)
                     /locus_tag="VGE92_02515"
     CDS             complement(7530..8990)
                     /locus_tag="VGE92_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY1312716.1"
                     /translation="MTRLCAVRWAGAVAWLAVAAACAVGPDYQRPKFDAAANYKEASN
                     WKPSEPNDVLNRGPWWEIFKDDALNQLEVRVDISNENVKAAAAAYDQARALVSQARAG
                     FWPTITGNASHLKSGSGASATTTSINGGTGVPISTTSRSPITDSAGLSASWDLDIWGK
                     IRRTTESDRASAQSSAAALAAARLSAQAQLATDYFELRAQDQLQKLLDDTVVAEQLSL
                     KITQSRYNFGVAARADVVSAEAQLLSSQAQQINAKIQRGILEHALAVLIGEQPANFSL
                     SPAAMRSDVPTVPAGIPSTLLERRPDIAEAERKVAAANAQIGVAKAAYYPDLTLNGSD
                     DYTSSTFSHLISAPNRIWSFGPSLAETLFDGGLRRAQVAQARAAYEGTVDTYRQTVLA
                     GFQQVEDEIVTLRVLEQQAVVEEAAVAAAREAEKLTLNQYKAGTVPYSSVITAQTTRL
                     AAEQTALSVLSTRLQASVALVEAVGGGWNAAQLPLP"
     gene            complement(8987..>11667)
                     /locus_tag="VGE92_02520"
     CDS             complement(8987..>11667)
                     /locus_tag="VGE92_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1312717.1"
                     /translation="TNDNARDAEPYRNLIIANRNGATVRLRDVATVLDMDSGATENIR
                     TYGLYNGKPAVFVQVFQQPGANIIEMIDAVKAELPALKESIDPTIDLVVTFDRSITIR
                     ASLRQVEQTLMLAVAFVILVVYVFLNSYRAALIPALVVPVSLIGTFGAMYLLGFTLDN
                     FSLMALTIATGFVVDDAIVVMENSTRHIEAGMSRMAAALLGAREVGFTVVSMSLSLVA
                     VFIPFMFAGGIVGRIFSEFTVTLSVSILISLVISLTTTPMMCSLLLRREDKNTASRFG
                     KAFDHAFERFRSNYVTTLDWALRHPRTMMIVLGATIGLNVFLYIVIPKGFFPQQDTGQ
                     LQGGLRGDAQSSFPFMKRKLQEVAAIVQADPAVQTVTGAVGGGGFGPSSGANANLNIA
                     LKPVSQRHVTADQIIARLRPKLNIVTGVSTFLQAVQDIRGGGRSANSQYQWTLLGDDL
                     SELQNWSQKLRTAMQDMPELTDVDTDLQPGGLEADLIVDRDSASRLGLTEIQIDNALG
                     DAFAQSQVSTIYNPFSPQQYHVVMEVAPEFWQNPDTLKDLYVSTSGGAVSGTQSTQAV
                     AGTTVLPSASSTAPSSAAVAADLARNLAANSLANAGRGNTSTGSAVSIAAETVVPFAA
                     FSHFSTGTTPVSVNHTGTSVSTSIAFNLPEGESLKTALDAIERKMADIHVPISIIGGT
                     YGTARLFQQSNSNTPLMLVAALVAIYVVLGVLYESYSQPLTILSTLPSAGVGALLALL
                     ATGTEFSLIAFLGILLLIGIVKKNAIMMVDFALEAERNLGMDPRTAIAHACNLRFRPI
                     MMTTCAAIAGALPLAIASGDGTEMRRPLGIAIVGGLIVSQILTVYTTPVVYLYVHRYW
                     KHLKQGKYASPPPLPPASAPPANPGGRGFDPLGAGA"
BASE COUNT         2117 a         3599 c         3820 g         2131 t
ORIGIN      
        1 gctcgcggcg cgcgccgcgc cgaacgccaa cggggtcggc tctgcggagc gcaatcgcgg
       61 ccccgcgccg atccgttcga cgcccatcac gttactggcc agacgcaacg tgccgctgtg
      121 gtcggcgctg accgaccaat ccgatccgcc gcatctgtcc tcccgggcac tggcggtggc
      181 cgaattcatc cgcgagcaag gtgcgtcgtt cttcgacgaa attgccgagc acgtgggcat
      241 gttgccggtg gaggtggagg gtgcactcgc cgaactcgtg gcgctcggtg tggtgaactc
      301 cgacagtttc gcaggcctgc gggtgctgct gctgccgagc ggccggcgca gcaagtccat
      361 gtcctacacc acgcgcaaca agcggcgcct cgcgctcttc ggcatggcgg acgccgggcg
      421 gtgggccttg gtgcgacgac ccgccacggc caccgcggcg cgccgcgacg aggcggtgga
      481 gcaaatcgtg cgcacgctgc tgcgccgctg gggcgtggtg ttctggaaac tgctcgcgcg
      541 cgaagcgggt tggttgccgc cgtggcgcga catactccag tgctgccgtc gattggaggc
      601 acgcggggag attcgcggcg ggcgcttcgt ggccggcttc tcgggcgagc agtatgcaac
      661 tcccgaggcc gtgggcctgc tgcgtgattt gcggcgcaag ccgctgacgc aaggctatct
      721 gtcgctgtcc gccgccgatc cgttgaatct gatcggcatc atcacgcccg gggcccgcct
      781 ggcgtcgctt gccggcaatc ggctgttgta ccgcgacggg ttgccgatcg ccacgtacgc
      841 ggccgatgag gtgcactaca tggaagaact ccccgagaaa gaacgctggg aagtgcaaac
      901 cgcgttgttg cggcgacata caccgctggc attggacgat ctggcctaag ccagcggcga
      961 tggatccgac ccggcgacgt caaggcggcc gcggcagacg cctcgatggc ggacgcgttg
     1021 cccagctgcc ctggagcgag gtcgacaatc cgtacccgcc gatggaggtg ctgtccgcgg
     1081 atcagctcga ggccattcac ctgacgtcgc tgcgaatcct cgaggatctc ggcatggagg
     1141 tgatgtcggc gcgcgcgctc gcggtactca aaacagcggg tgcgcaagtc gacgcagcgg
     1201 caagaatcgt gcgcctcgat cgcgcactgg tcgaacgcgc gctcgacacc gcgccccggt
     1261 ccttcgtgct caccccgcgc aatcctgcca agcgcgtgac cctgggcggc agccgcatca
     1321 acttcggctt ggtcgcaggg ccgcccaacg tccacgactg cgagcgcgga cgccgctccg
     1381 ggaactaccg cgactactgc gacttcatcc ggctggcgca gtacttcagc gccatccatc
     1441 tgatcggcaa ccaagtgtgc gcgcccatcg agctgccggc caactcgcgt catttggata
     1501 cctaccgcgc gaatcttgga ttttcggacc aggtgtatca ctgcaccgcg atcggcgcgg
     1561 gacgcgcgct cgacggcata cgaatgatgg cgatcgcgcg cggcctttcc ttggacgaga
     1621 tcgcggcaag cccgggcgtc atggccatta tctcggtgaa cagtccgcgc cgcttcgatg
     1681 aggcgatgac cgaagggctg atggcgatgg cggaacacgg ccaggccgtg gtcgtcacac
     1741 cgttcacctt gatgggcgcc atggcgccgg tgtcgcttgc cgcagccctg gcgcagcaaa
     1801 acgcggaggc gttgttcggc gtggtgctcg cgcaattgat caggccgggc accccggtca
     1861 tgtacggtgc cttcacctcg aacgtcgaca tgcgctcggg agcaccggca ttcggcacgc
     1921 cggaaaacgc caaggcgaat atcgccagcg gacaattggc gcgccgctac cgtctgccct
     1981 atcggacatc gaacgcgagc gcctccaacg ccgccgacgc gcaaggcgct tatgagacgc
     2041 agatgtcttt atggggagcg atcctgggac acggcaatct cgtgtaccac gccgccggct
     2101 ggcaggaagg tggactgacc gcctcgttcg agaagctggt gttggacgtc gaaatgctgc
     2161 agatgatgat cgagtttctc aagcccatcg tggtcgatga gcgcgaactc gggttcgatg
     2221 ccatcaaggg agtcgcaccg ggcggccatt ttttcggcga agcgcatact atggagcgct
     2281 acgagcacgc attctatcgg ccgctgctct cgaactggca aaactatgag aattggcagc
     2341 tcgccggcgc caaggacgcg acgcagcgtg caacgcaaac ttggaagcag gcgctccatg
     2401 agtacgaaca acccgcgatg gatgccgcga tcggcgaggc gctcgacgcc tacgttgcca
     2461 ggcggcgcag cgagatcggc agcaacgatc cttgagatgt atcactagag gagtccatcc
     2521 gtgttcgaac cgttcccgaa aaactatatt tggaacctgc agaccaatct ggcgctcatc
     2581 tgcggcggca atcacggtga aatcgacgaa gcgtgccgtc cactgcgcga tgcggccgcg
     2641 cgcggcgccg atgccggtac cgcgttgttg ttcgatagct ggatcgcggt ggcggatcag
     2701 gtcgcgcgca acgccgaggc ggacgagaag gccggctttc gcttgagcgc cggcacgaaa
     2761 ttcgggcggg cctcgggtta ctacttgtcg gcggagcgca tgcagtcgcg cgattatgcg
     2821 ccgcgctggg tggcgtatga gcgcggactt gcactctacc gcaagcatgt ggccctgcgc
     2881 ggtctgcacg tcgacttcgt cgacatcccg tatggcgatg cgagttatcc ggccattttc
     2941 gtgcatgacg gctccgctac cccgcgtccc gcgctcatct ccgtcaacgg cctcgacagc
     3001 atgaaggaac aggtgaacat gggcgggcac ggccagtcca atctggaacg cggcatcaat
     3061 acgctgttcg tcgatcagcc cggcaccggc gaagcactgc gcaagcgcaa actcaccgcg
     3121 gtctatgaca gcgaacgctg ggccacgccc gcactcgagt atctgctcac gcgcagcgac
     3181 gtcattccca acaaggtggg aatttttggc ttgtcgctcg gcggctacta cgcgccgcgc
     3241 gccgccgcga atgagccgcg atttgcgctg tgtgcggtga tgggggccaa ccatctgtgg
     3301 ggagacttgc agaggcgccg cttggcgcgc gagggcgaga acccggtgcc gcactattgg
     3361 gaccacgtga tgtgggtgtg gggcaagaaa tcgctcgacg agttcatggc ctacgtgccg
     3421 caaatcaccc tcgacggcca gatcgagcgc atgaaaatgc cgtttctgat catgcacggc
     3481 gcgggcgacc ggcagattcc ggtgagcgat gcgcaccgca gctacgagca ggcagtgaac
     3541 agccgcgcgc gcgaactacg aattttcacc aaggcggatt tcgaagtcga gcattgcggc
     3601 gccgacaatg gctgcggtat gcgcgactac gccgccgact ggtgtgcgca gaccttcgcc
     3661 aagatcccgc cgccgtaatg cgccgcgatt cgcagcgctt gatcgcgctg acgctcgcgg
     3721 ctgtcgcgct cgcagcgtgc cactcattgg cctgtgccgc cgaagccgta tcggccatgc
     3781 cggttccggt gccagaaccc tcgccgcaag ccatacgcct ctatcagagc ggcaacgtgc
     3841 tgtgggtggt cgcgacgctg tgggggttca tggtgccggc gctgattttc tttagcggct
     3901 tgtctgctcg cctgcgggat gcggcacggc gcagagcgtc acgctggtat gcgtcgctgc
     3961 tgatctacct ggtgttgctt gcgtcgctgt tgttcttggc gaatctgccg ctcgattatt
     4021 attccgattt tctgcggccg caccagtatg gattgtcctc gcaggcgttt gcaaagtggc
     4081 tgcaggacga gatcctgaac ctcggcctcg gcctcattgg cgcagccttg gtgctgtgga
     4141 ttccgtatct gctgctcaag cgcgcgcgcc gacgctggtg gctgtatacc tggtttgcca
     4201 gcatcccgat cttgattttc ctcaatttca tcgggccgat ttggattgcg ccgcttttca
     4261 atgattttgg cccgatgcac gacaaagcgc tggaaacgcg gattttgcaa cttgccgatc
     4321 gcgccggcat cgacggggcg gctgtctatg aggtgaacaa gagcgtcgac agcaaacagc
     4381 tcaatgccta tgtcgcgggc atcggcagca caaaacgcat cgtgctgtgg gataccacgc
     4441 tcaaggaact caaccccgat gaagtgctga tggtcatgag tcacgaaatg ggccattacg
     4501 cgctcgggca tgtgtggaag ggcctcattt atgaaagcct ggtgctcctg gtgggcttct
     4561 ggtgcgtgca tatcggcgtc gaggcgatca tccggcgtta tggtgcgcgc acttcggtgc
     4621 acgagcccgg cgacttcgcc tcgctgcctt tgttcatgct gatattcagc gccgtcgggt
     4681 tcgtactgac cccgccgctg ctcgccgttt cacggcacta cgagcacgag gccgatcgct
     4741 tcggcttgga gatgacgcac gagaatcgga gctgcgcgct ggttttcgtc aaattcatag
     4801 aacacgacct ggcgtatccg ctgccttcgc cgctcgtcat gttcttgcgt gcgtcgcatc
     4861 cttccgcagg tgaacgcatc aatttttgca acacctatca tccctggctg caagggcagg
     4921 agggacgcta cacccattac ttcaagccga tgcccgcgga ttaggcgccg gtcgacttgc
     4981 gccgcatgcg gcccgcaaag cgggtgaacg ccgagtcggt cacgtcgcac gccgcgcccg
     5041 cagcagcgcc cgctacgagc gaccccgggt catcggcaac cagatggcgg cgcgcgtgat
     5101 cggcaccgag gaggcaccgt ggcccgcctt gccgacatca atgtagaaca tcttgggttg
     5161 cgttcgcagc tccttgggaa cgagcttgat gaaaaagctg gtccattctc cgccgcgcgg
     5221 gaaggtctga atcgctttct cttgatcgac ataatcaacg ccgggcttgg cggacgcggc
     5281 ctcggtccac catacgaagg aactatcgcc gggcatgagt gaagtacgat gaacgcgaac
     5341 ctccgcaaag ttctcgccgg cgcgcacgcg gtagttcttg cgggacacga cgattggcag
     5401 agcggcggct gccgcgggtg gcttggcgtt cgcgactgcg ccggcggtga gcggcgcagg
     5461 aggattggag gtctcaaggc tggcggcggc gatatcggcc acaggcttgg ccggaggttg
     5521 aacgattgcc gccgccagcg gagcgattgc actgccggcg ctgagccccg tcgcccagtg
     5581 tgcgagcgcg acccaggcgg cggccgtgaa catgatcgcg acaccgagcg cggcggttcg
     5641 cgcgccgagc cgcggtgcag gcacggagcg cgccggccgc tcgccgagca aatcatcgag
     5701 ccacgcgcgc gtcgacgagg gccgagccgc ccgacaccac gacaaggcgc atgccaaggc
     5761 cttccattgt tgccgcttga gcccgcgcgg ccggctggcg atgatcccat ggtcgcgcgc
     5821 cacggtcgcc gggcggcgct gaaatggatg cgacccggcg atcaattcgt aggacaggca
     5881 ggcgaaagcg aacagatcgt cacgcgggtc ggccgcgcgg ccggccaaga gttcgcagct
     5941 cgcataggca ggcgtgagcg gaggatacgt attgcccggg tgcattgcat ccgcggcaac
     6001 ttgtgccgtg cggaagctcg atgaaccgaa atcgaggatc cgcacgtcgc cgcacggcgt
     6061 aatcatgatg ttgtggggtt tcaagtcgcc atgaatgacc tgtcgggcgt gggcgtgcgc
     6121 caaacccgca ccgatctcgc ggatgatgtg ccaggcatag ggacgcgcca tgggcgcgcc
     6181 tttgagacgg tccatgacgc tgctcaagag ttcgccttcg agcaactcca tcgtatagaa
     6241 atgcaattca gcgtcgcggt cgagttcgtg cactttgacg acgttgcggt gcgagagcat
     6301 ctgcgtgcag taaaattcgc gtcgcaaagc ggcaagttgc tcggactcgc agccgcccgt
     6361 gtcattcaga aattttatcg ccacgtattg gtgtgtttcg ggaagatcgg agcggtaccg
     6421 atcgagcgcc ttgtatacgg tgccgcggcc accgcttccg agccgctgct cgatcacata
     6481 gcgatcgcgc agcactcgac cgatgtcgac cgcaaaaggc agatgttgct cgagcgcggc
     6541 actgcttgcc cggggagccg cgcaggttcg aagatcgacc gtctgagcat gtgttgcgtc
     6601 gaagccgcta acttgttcag gcgatttgag actgagtgac atgccgacgg cgacccctgc
     6661 gtcgttaaac ggaaacactg ttgtgtgaaa accacgaatg ccgaggcact aatgtgcaac
     6721 cgaatcggac cgctgtatgt cagacaacgc cgcatatcga gcgttgcagc ccatgacaaa
     6781 tctgtcaggt gcgaagccac ggccgcagga ctcgccgcgc cggccggtcg acggtcgaga
     6841 tcacggcggg atccaacagc cggcgccgac gcagatgccg aatcgcagcc tgaacagtcg
     6901 atttggcaag ccctgtcttg gtcgccacgg tttgtaggct gacggcgacg gtgtcatgcc
     6961 ggcttttcgc ggccagccgc aacaggtaca gataaacgat gaacgccgcg gggcggcggt
     7021 cgtgccccac cagatccggc atcagcacgt caagcacata ggcgtcgagg tgaatgcgat
     7081 ccatattgat tatcactata gcattgtttg ctacaggagg cgcgtccgat gcgccatact
     7141 ggagttgctg cccgttagga aattgacatg atcaagcaac tcaaattcgt ctcgataccg
     7201 acgcgcgacc aagcccgggc ggtcaagttc tacaccgaac agctcggctt cgaagtggcg
     7261 acggatcagc ccttcgacga tcgtcagcgc tggattgagc tgcgtatcgc caacgcagcg
     7321 acccgcgtcg tgctgttcac gccgccaggc caagaggacc gggttggcac cttcttcaac
     7381 ggctcgttcg aatgcgatga cgtcgaggcg acctatcgtc aactcacgca acgcgggggc
     7441 gttttcgtgc gcgagccgga gaaacagccg tggggcacga tggcggtctt ccgcgacccg
     7501 gacggcaacc agtttgttct atcgtcgggt tagggcagcg gcaattgcgc cgcgttccag
     7561 ccgccgccga cggcctcgac cagggcgacg ctcgcctgca agcgcgtgga cagcacgctg
     7621 agtgcggtct gttcggcggc gaggcgggtc gtttgcgcgg tgatcacgct gctgtagggc
     7681 acggtacccg ccttgtactg gttgagcgtg agtttctcgg cttcgcgggc cgctgccacc
     7741 gccgcttcct cgaccacggc ctgctgttcg agcacgcgca aggtgacgat ttcgtcttcg
     7801 acttgttgaa atcccgcgag cacggtctgg cggtacgtat cgacggtacc ctcataggcg
     7861 gcgcgcgcct gagcgacctg cgcgcggcgc agacccccat cgaacagggt ttccgccagc
     7921 gatgggccga atgaccagat gcggttcggc gcgctgatca aatggctgaa ggtgctgctg
     7981 gtgtaatcgt ctgagccgtt cagcgtgagg tcgggataat aggccgcttt ggcaaccccg
     8041 atctgcgcat tcgcagcggc aactttgcgt tctgcttccg cgatatccgg tcgccgctcc
     8101 agcaaggtcg aagggatgcc cgcgggcacg gtcggcacat cgctgcgcat ggccgctgga
     8161 ctcagcgaaa agttggccgg ctgctcgccg atgagcacgg caagcgcatg ctcgaggatg
     8221 ccgcgctgta tctttgcatt gatctgctgc gcctgactcg acagcagctg cgcctcggca
     8281 ctcacgacat cggcgcgcgc ggccacaccg aagttgtagc ggctctgcgt gatcttgagc
     8341 gacagctgct cggcaaccac cgtgtcgtcc agcagtttct gcagctggtc ctgggcacgc
     8401 aattcgaaat aatcggtagc cagctgcgcc tgcgccgaca gccgcgccgc ggccaatgca
     8461 gctgcgctcg attgcgcgga ggcgcggtca ctctccgtgg tacggcgaat cttcccccaa
     8521 atatccaagt cccaactcgc cgacaggccg gcgctgtcgg tgatcgggga gcgggaggtg
     8581 gtgctgatcg gcacgccggt gccgccattg atgcttgtgg tggtggcgct ggcccccgag
     8641 ccgctcttca gatgcgaggc attgccggtg atggtgggcc aaaagcccgc gcgtgcctgc
     8701 gacaccagcg cgcgcgcctg atcataggcc gcggcagcgg ccttgacgtt ttcgttcgaa
     8761 atgtcgacgc gcacttcgag ttgattcagc gcatcgtcct tgaagatttc ccaccagggg
     8821 ccgcggttca agacatcgtt gggctcgctc ggtttccaat tggaggcctc cttgtaattc
     8881 gcggcggcgt cgaatttcgg gcgctgatag tcaggcccca ctgcgcacgc cgcggccaca
     8941 gcgagccacg cgacagcgcc cgcccaacgc acggcgcaga gccgcgtcat gcgccggctc
     9001 cgagcggatc gaatccgcgg ccacccggat tcgccggcgg cgccgaggcc ggcggcaacg
     9061 gcggtggcga agcgtacttg ccctgcttca gatgcttcca atagcgatgc acgtacagat
     9121 aaaccaccgg cgtcgtgtac acggtcaaga tctggctgac gatgaggcct ccgacaatgg
     9181 cgatgccgag cgggcgacgc atttcggtgc catcgcccga ggcaatggcg agcggcaggg
     9241 cgcccgcgat ggcagcgcag gtagtcatca tgatgggccg aaagcgcagg ttgcaggcgt
     9301 gcgcgatggc ggtacgcggg tccattccga ggttgcgctc ggcctcgaga gcgaagtcga
     9361 ccatcatgat ggcatttttc ttgacgatgc cgatcagcag caagatgccg aggaaggcga
     9421 tgagactgaa ttcggttccc gtggcgagca gtgccagcag tgcgccgact cccgccgagg
     9481 gcagggtcga cagaatggtg agcggctggc tataactttc atagagcacg ccgagcacga
     9541 cgtagattgc gaccaacgcg gcaacgagca tcagcggcgt attgctgttg ctctgctgga
     9601 acaggcgcgc ggtgccgtaa gtgccgccga tgatcgagat gggcacatgg atatcggcca
     9661 tcttgcgctc gatcgcatcg agggcggtct tcagcgattc accttccggc aaattgaacg
     9721 cgatggaggt cgacaccgag gtcccggtgt ggttcacgct caccggcgtg gtgccggtcg
     9781 agaagtgact gaacgccgcg aacggtacca cggtctccgc agcgatgctg accgccgaac
     9841 ccgtcgaagt gttgccgcgg ccggcattcg ccagggaatt cgccgcgagg ttgcgggcga
     9901 gatccgccgc aaccgcggcg ctcgaggggg cggtgctgct ggcggagggc agcacggtgg
     9961 tgcccgccac ggcctgcgtc gactgagtgc cgctcactgc gccccccgag gtgctgacgt
    10021 acaggtcctt gagcgtgtcc gggttctgcc aaaactccgg cgccacctcc atcaccacgt
    10081 gatattgctg cgggctgaag ggattataaa tcgtcgacac ttgagattgc gcaaatgcat
    10141 cgccgagcgc gttgtcgatc tggatttcgg tcagcccgag ccgcgaggcg ctgtcgcgat
    10201 cgacgatcag gtcggcttcc aggccgccgg gctgcaaatc ggtatcgacg tcggtgagtt
    10261 cgggcatatc ctgcatcgcg gttcgaagtt tctgcgacca gttctgaagt tccgacagat
    10321 catcgccgag gagcgtccat tgatattgcg agttggcgga gcggccgccg ccgcgaatgt
    10381 cctgcacggc ttgcaagaag gtggagacgc ccgtcactat attcagtttg gggcgcaacc
    10441 gggcgatgat ctgatccgcg gtgacatgcc gttggctcac cggcttgagg gcgatgttga
    10501 gattcgcgtt ggcgccgctc gagggaccga agccgccgcc gcccacggcc ccggtgacgg
    10561 tctgcaccgc cggatccgcc tgaacgattg cggctacttc ctgaagtttg cgcttcatga
    10621 atgggaatga gctctgcgca tcaccgcgca gcccgccttg cagttgcccg gtgtcttgct
    10681 gcgggaagaa gcccttgggg atgacgatgt acaggaagac attgaggccg atggtggcgc
    10741 cgagcacgat catcatggtc cgcggatggc gcagcgccca atcgagcgtg gtcacatagt
    10801 tgctgcgaaa ccgctcgaat gcgtggtcga aggccttgcc gaatcgagac gccgtattct
    10861 tgtcttcacg ccgcagcagc agcgaacaca tcatcggcgt cgtcgtcagg gagatcacca
    10921 gcgaaatcag aatcgatacc gacagcgtca ccgtgaactc gctgaaaatg cgtccgacga
    10981 tgccgccggc aaacatgaac ggaatgaaca ccgcgaccag cgacaggctc atggacacga
    11041 cggtgaagcc cacctcgcgg gcgcccaaca gcgcggccgc catgcgcgac atgccggcct
    11101 cgatatgccg cgtggaattc tccatgacga cgatggcgtc atccaccacg aagccggtgg
    11161 cgatggtgag cgccatcagc gagaaattat ccagcgtaaa gcccagcagg tacatggcgc
    11221 cgaaggtgcc gatcagggat accggcacca ccagagcggg gatcagggcc gcgcgatagc
    11281 tgttcagaaa cacatagacc acgaggatga cgaaggccac cgccagcatc agcgtctgtt
    11341 cgacctggcg cagcgaggcg cgaatcgtga tggaccggtc gaaggtgacg accaggtcga
    11401 ttgtcgggtc gatcgattcc ttgagcgcgg gcaattccgc cttgacggca tcgatcatct
    11461 cgatgatgtt ggcgccgggc tgctggaaca cctggacgaa gaccgcgggc ttgccgttgt
    11521 acaggccgta ggtgcggatg ttctcggtcg cgccgctgtc catgtcgagc accgtggcca
    11581 catcgcgcag ccgcaccgtt gcgccattgc gattggcaat gatcaggttg cgatacggtt
    11641 ccgcgtcgcg cgcattgtca ttggtgt
//
LOCUS       DASWZT010000096         5673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_15180, whole genome shotgun sequence.
ACCESSION   DASWZT010000096 DASWZT010000000
VERSION     DASWZT010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15180"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VGE92_02525"
     CDS             <1..671
                     /locus_tag="VGE92_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEY1312718.1"
                     /translation="VEQIRRSGLAYALYLWCTTTLADGLCALNGLRIGLRMSSLPGDP
                     PPRRLTTRDLNDPEGRAFLAACAPQLLVSSFFNQRLRAETLAVPTVASLNIHPSLLPD
                     AKGVDPVFQSLLHGGPALGVTVHFMNPEFDAGQILAQQPVEARPGASVFELTALLYRV
                     GAELLASAVIRVAAGDKGLAQPGAGTYQSWPTAAETAALRRRGGALLRFMDLIRLARA
                     RLPR"
     gene            complement(683..1675)
                     /locus_tag="VGE92_02530"
     CDS             complement(683..1675)
                     /locus_tag="VGE92_02530"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY1312719.1"
                     /translation="MIIPRRKLGPLGLEVSAIGLGCMGMSDFYGPSDEATNLRVLNAA
                     VDIGSNFLDTADMYGVGANERLLAKVLKTRRAEIVLATKFGNVRAADGTSLGINGTPE
                     YVRAACDASLERLGVDHIDLYYQHRVDPKVAIEETVGAMAELVKAGKVLHLGLSEASA
                     KTIRRAAQVHPITALQSEYSLWSRDLEATVLPTCEELGIGVVAYSPLGRGFLTGAFKT
                     PEDFEAGDYRRSSPRFSSENFAGNLQLVEIVAQIAREQGFTPAQVALAWLLDCAPYIV
                     PIPGTRNIQRLKENALSVMVRLSDAQLERLYGILRERPVSGARYPAASMVTIDA"
     gene            1844..2782
                     /locus_tag="VGE92_02535"
     CDS             1844..2782
                     /locus_tag="VGE92_02535"
                     /inference="COORDINATES: protein motif:HMM:NF018431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY1312720.1"
                     /translation="MFDSSLQRILATTGMAERQQELSVRVARLTPFDGTHQSVLPPLA
                     LARASVPTVCMPTVYQPCLAIVVQGRKRAVLNDDVFQYDALNYLVVSVTLPGMGQVLE
                     ATPEHPYLSLRLNLDLEEIARLVLELGDRGASPAAADRGLFVARLDEPLLDAVLRMVK
                     LLDTPEDIGVIAPLVQREIYYRLLRGELGHRMVDLAQSEGANHRIVRAIEWLKQHYTA
                     PLRIEALADAVHMSPSALHHRFKAVTAMSPLQYQKQLRLHEARRLMFADGIESATAGH
                     RVGYESASQFSREYRRLFGAPPRSEIARLREAGASA"
     gene            complement(2787..5294)
                     /locus_tag="VGE92_02540"
     CDS             complement(2787..5294)
                     /locus_tag="VGE92_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY1312721.1"
                     /translation="MTQPMENFVRVRGARDHNLKNVNVDIPRNALVVFSGISGSGKSS
                     LAFGTLYAEAQRRYFESVSPYARRLMDQIAVPDVDSIDGLPPAVALQQQRGATSSRSS
                     VGSLTTLSNLLRMLYSRVGDYPRGQPMLYAENFSPNLPQGACPNCHGLGHVYDVTEAS
                     MVPNGSLTIRERAIVAWPPAWHGQNLRDILVTLGYDVDTPWRKLPKKDRDWILYTDEQ
                     PTVPVYAGYTPAQTRRALRRKEEPSYQGTFTGVRKYVLQTFRTTQSALMKKRAARFMS
                     ATLCPVCQGKRLTRAALSVTFAGADIAALSHLPVGELVALLAKTVARGVGRDPWPPEK
                     RLAAQRIVANFQERVATLQHLGLGYLSLERATPTLSPGELQRLRLATQIRSNLFGVVY
                     VLDEPSAGLHPADTQALLQALQQLKLAGNSLFVIEHDLDVMRRAEWIVDVGPGAGANG
                     GRVLYSGPLGGLREVSESQTARYLFAAVQPVSRQARTPRGWLELKNVTRNNLRELNVS
                     FPIGVLTVVTGVSGAGKSSLVSEALTDLLADRVVGPKAAIQEDVTEEPVLRAAQFTQG
                     SIGAGADSVQRVVAVNQKPIGRTPRSNLATYTGFFDEVRRLFAATASAKARRYSASRF
                     SFNVDGGRCPTCQGEGFVSVELLFMPSVYAPCPVCHGTRYHESTLKVLFHGKNIAEIL
                     TMSVDLACEFFAEYPAIQRPLRLLQDIGLGYLKLGQPATELSGGEAQRVKLATELQRV
                     PRGATLYVIDEPTIGLHPADVDKLMIQIEAFVDAGNTVILAEHELRVAALADWIIDMG
                     PGAGDAGGRIVAAGTPQAITANPASKTGVYLAPLLRQ"
     gene            complement(5492..>5673)
                     /locus_tag="VGE92_02545"
     CDS             complement(5492..>5673)
                     /locus_tag="VGE92_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312722.1"
                     /translation="QEYLAKLEHIRAAVAHHMFEEEGIWFPELTKTVLSCDQVKLSRR
                     YQEEFTRYVGQDQAA"
BASE COUNT         1050 a         1773 c         1764 g         1086 t
ORIGIN      
        1 cagtcgaaca gattcggcgc agcgggctcg cgtatgcgct gtacttgtgg tgtaccacca
       61 cgctcgccga tggtctttgc gcactgaatg gcttaagaat tggcttaaga atgtccagcc
      121 tgcccgggga cccgccgccg cgccgattga ccacccgtga cttgaacgat ccagaaggtc
      181 gcgcattctt ggcggcctgc gcaccgcagc tgctcgtgag ttcgttcttc aaccaacgct
      241 tgcgcgccga gacgctcgcc gtacccaccg tggcgagctt gaacatccat ccctccttgc
      301 tgcccgacgc caagggggtg gatcccgttt tccagtcgct gctgcacggc ggtccggcgc
      361 tcggcgtcac ggtgcatttc atgaatccgg agttcgatgc gggtcagatc ctggcgcaac
      421 agccggtcga agcgcgcccg ggtgccagcg tgttcgaatt gaccgcctta ctgtatcggg
      481 tcggtgcgga actgctggcg agcgccgtca tccgggtagc cgcgggcgac aagggacttg
      541 cacaacccgg tgccggcact tatcaaagtt ggccgactgc ggccgagacc gctgccctgc
      601 ggcggcgggg cggcgcgctg ctgcgcttca tggacttgat ccgattggcg cgcgcacgcc
      661 ttccgcgtta gcggctcgca tcttaggcgt cgatcgttac catcgatgcg gcggggtacc
      721 gtgctccgct cacgggccgt tcacgcaaga tcccgtacag acgctcgagc tgagcatcgc
      781 tcagccgcac catgacagac aatgcatttt ctttcaggcg ctggatgttg cgcgtacccg
      841 gaatcggcac gatgtacggt gcgcagtcga gcagccaggc aagggcgacc tgtgccggcg
      901 taaacccttg ctcgcgtgcg atctgcgcta cgatctcgac cagctgtaga ttgccggcga
      961 aattctcgct cgagaatcga ggactgctgc gccggtagtc gccggcttcg aagtcctcgg
     1021 gcgttttgaa cgcaccggtc aaaaaaccgc gcccgagcgg gctataggcg acgacgccga
     1081 tgcccagctc ctcgcacgtg ggcaggactg tcgcctccag gtcgcgcgac cagagtgagt
     1141 attcgctctg taatgcagtg atggggtgca cctgcgcagc gcgtcgaatg gtctttgcgg
     1201 aggcttcgga caggcccaag tggagcacct tgccggcctt gaccagctcc gccatggccc
     1261 cgaccgtctc ctcgatcgca accttggggt cgacgcggtg ctgataatac aaatcgatgt
     1321 gatcgacccc gagccgctcg agactcgcat cgcaggccgc gcgcacatac tccggtgtgc
     1381 cgttgatacc gagagaggtc ccatcggcag cgcgcacgtt gccgaacttg gtggcaagta
     1441 cgatttccgc gcggcgcgtt ttcaatactt tcgcgagcag ccgttcgttg gcacccacgc
     1501 cgtacatatc ggcggtatcg agaaagttgc tgccgatatc gacagcagca ttgaggacac
     1561 gcagattggt ggcctcatcg gacgggccgt aaaagtccga catgcccatg cagccgagac
     1621 caatggccga gacttcaagg ccgagtgggc cgagtttgcg acgcggaatt atcatgctct
     1681 ctccaaggcg gggggtgctg ttcatgctaa ccaccgaacc ccagggacgc tagatcatta
     1741 ctgccgggtt cttgcctatt cctgcaaaga ggccgatcct agctatcgca ccggatggcg
     1801 agcgattacc atgcatgaat cattcaagga tcgataaata ttcatgtttg acagtagttt
     1861 gcagagaatt ctcgcgacca ccgggatggc cgagcggcag caggagttgt cggtgagggt
     1921 tgcccgattg acgcccttcg atgggaccca tcaatcggtg ttgccgccgc tcgcgctggc
     1981 ccgcgccagc gtgccgacgg tgtgcatgcc cacggtgtat caaccgtgtt tggccatcgt
     2041 cgtgcagggc cgcaagcgtg ctgtgttgaa cgatgacgta ttccagtacg acgcgctcaa
     2101 ttatctcgtg gtatccgtga cactgccggg gatgggtcaa gtgctcgaag ccactcccga
     2161 gcatccctac ctctccctgc gtctgaacct tgacctcgaa gaaatcgcgc gcttggtgtt
     2221 ggaacttggc gatcggggag cctcgcctgc tgccgcagac cgcgggctat tcgtcgcgcg
     2281 tttggacgag ccattgctgg atgccgtctt gcggatggtg aagctgctcg atacgccgga
     2341 ggacatcggc gtgatcgccc cgctcgtcca gcgcgaaatc tattatcgcc tgctgcgcgg
     2401 cgagttgggc catcgcatgg ttgatctcgc acaaagcgag ggtgccaatc atcgcatcgt
     2461 tcgcgccatc gagtggctca agcagcacta tacggcgccg ctacgcatcg aggcactggc
     2521 ggacgccgtg catatgagcc cgtcggcact ccatcatcgc ttcaaggcgg tcaccgccat
     2581 gtcgccgctg cagtaccaga agcagttgcg gctgcacgaa gcgcgccgct tgatgttcgc
     2641 cgacggcatc gaatcggcga ccgcagggca ccgcgtcggc tacgaaagtg cctcgcagtt
     2701 cagccgcgaa taccgccgcc tgttcggcgc tccaccccga tcggagattg cgcgcttgcg
     2761 cgaagcgggt gcgtcggcgt agcacctcac tgccgcaaca gcggtgccag gtagacaccg
     2821 gtcttgctgg cgggattggc cgtaatcgcc tggggcgtgc ctgcagcgac gatccggcca
     2881 ccggcgtcgc cggcgccggg tcccatatcg ataatccaat ccgccaacgc ggcgacgcgc
     2941 agttcgtgct cggcgaggat gacggtattg ccggcatcaa caaaagcttc gatctgaatc
     3001 atcagcttgt ccacatcggc cggatgcaga ccgatcgtcg gctcatcgat gacgtacaag
     3061 gtcgcaccgc ggggtactcg ctgcagctcg gttgcaagct tgacgcgttg cgcctcgccg
     3121 cccgagagct cggtcgcggg ctgcccgagt ttcaagtacc caaggccgat gtcctgcagc
     3181 agtcgcagcg gccgttggat ggccgggtac tctgcaaaga actcacaggc cagatccacg
     3241 ctcatggtca gaatctccgc gatgttcttg ccgtgaaaca ataccttgag ggtggattcg
     3301 tgatagcgcg ttccgtgaca gacgggacag ggcgcataca cactcggcat gaacagcaac
     3361 tcgacactca cgaagccctc accttggcag gtcggacagc ggcctccgtc gacgttgaag
     3421 gagaaccggc tggcggagta gcgtcgcgcc tttgccgagg cggttgcggc aaacagccga
     3481 cgcacctcat cgaagaatcc cgtataggtc gccaaattcg atcgcggcgt gcggccgatt
     3541 ggcttttgat tcacggcaac cacgcgttgc acggagtcgg cacccgcacc gatgcttccc
     3601 tgagtgaact gcgcggcacg cagcaccggc tcctcggtta cgtcctcttg aattgccgct
     3661 tttgggccaa ctacgcggtc cgcgagcaag tcggtgagcg cctcactcac cagactcgat
     3721 ttaccggcgc ccgacacccc cgtcaccacc gtcaacacgc cgatcggaaa cgatacgttg
     3781 agctcgcgca gattattgcg ggtgacgttc ttcagttcca gccagccgcg cggggtgcgc
     3841 gcttgccggg acaccggttg aacggcagcg aacaaatatc gcgccgtctg cgactcggaa
     3901 acctcgcgca ggccgccgag cggcccgctg tagagcaccc tgccgccatt ggcgccggcg
     3961 cccggtccca cgtccacgat ccactccgca cggcgcatga catcgagatc gtgttcgatc
     4021 acgaacaaag agttgccggc gagcttcaat tgctgcaatg cctgcagcag cgcttgggtg
     4081 tcggcaggat gcagccccgc ggatggctca tccaacacgt acaccacgcc gaataaattg
     4141 gagcggattt gggtcgcaag gcgcagccgc tgcaattcgc cgggcgacag agtgggcgtg
     4201 gcgcgttcga gcgacagata gccgagtccc aagtgctgca gggtggctac ccgctcttgg
     4261 aagttggcga cgatgcgctg tgccgcgagg cgcttttccg gcggccaggg atcgcggccc
     4321 acaccgcgcg cgacggtctt ggcgagcagc gccaccagtt cgcccacggg taggtgggag
     4381 agtgcggcga tatcggctcc ggcaaaggtg accgacaacg ccgcacgcgt caaacgtttg
     4441 ccctgacaca ccgggcagag cgtcgcgctc ataaaacgcg ccgcacgttt cttcatcaac
     4501 gcgctttgcg tggtgcgaaa agtttgcagc acatacttgc gcaccccggt aaaggtgccc
     4561 tgatagctcg gctcctcttt gcgccgcagg gcgcgccgag tctgcgcggg cgtgtagccg
     4621 gcgtacaccg ggaccgtcgg ttgctcgtcg gtatacagga tccagtcgcg atcctttttc
     4681 ggcagcttgc gccacggggt atcgacgtcg tatccgagcg tcaccaaaat atcgcgcaga
     4741 ttctggccgt gccaggcagg gggccacgcg acgattgcgc gctcgcggat ggtcagagag
     4801 ccatttggaa ccatcgacgc ctcggtgaca tcgtagacat gcccgagacc gtggcagttg
     4861 ggacaggcgc cttgcggcaa attcggcgag aagttctccg catacagcat cggctggcca
     4921 cgcggatagt cgcccacgcg tgagtaaagc atgcgcaaaa gattcgacag cgtggtgagg
     4981 cttcctaccg aggaacgact gctggtcgcg ccccgctgtt gctgcaaggc gacggccggc
     5041 ggcaagccgt cgatcgaatc gacatccggc accgcgatct ggtccatgag tcgacgcgcg
     5101 tacgggctga ccgattcgaa gtaacgccgt tgcgcctccg cgtacagcgt cccgaaggcc
     5161 agcgacgatt tgccggaccc ggaaatgccg ctgaacacga cgagtgcgtt gcgcggaata
     5221 tcgacgttga cgttcttgag attgtgatcg cgcgcgccgc gcacgcgcac gaaattttcc
     5281 atcggttggg tcatgctgct gccacaaccc gttactttac ggtcttgcgg ccggcatttc
     5341 attgccagcc ttggtgtacc cgggcggaga atagaaattc agcgttttgt gggaccgccc
     5401 tcagaatcgc cgggtgcgat gacgattttg cgccgcctgc acacggcggg ccaaactgca
     5461 gtgtcttcac tttcatgccg gggacagggt gttatgcggc ctgatcttgc cccacgtagc
     5521 gcgtgaactc ctcttgatag cgtctcgaga gtttaacctg atcgcaagaa agcactgtct
     5581 tggtgagttc cggaaaccag atcccctctt cctcgaacat gtggtgagcg acggcagcgc
     5641 gaatgtgctc gagcttagcg agatactctt ggc
//
LOCUS       DASWZT010000097         3730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_73275, whole genome shotgun sequence.
ACCESSION   DASWZT010000097 DASWZT010000000
VERSION     DASWZT010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73275"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1297)
                     /locus_tag="VGE92_02550"
     CDS             complement(<1..1297)
                     /locus_tag="VGE92_02550"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEY1312723.1"
                     /translation="MAVTAVGRAVICVAAIFFGASSSLVMADGPPKNPQEIYGPRVQP
                     ATKDVFRYTAHRRSNASVDALRYWNQLAVDATGLDHTPVAPGENRVYGEQLGPTRSSR
                     AMAIVHVSMFESVNSILGGYRSYLGLPRAREPASVDAAIATSAHDTLSALFPSQRPNF
                     DALYAQELNRIPSRDGAMKNRGVAIGHLAAAGILALRADDGSAVPDPKLGTEWVTGNE
                     PGDWRQDPIALSPIALGAYWGEVKPFVLRSAIQFLATPPPALTSPEYTAAYDEVKSLG
                     GDGVHTPTQRTAEQTYIGIYWAYDGTPSLCAPPRLYNEITTEISKSSSSVSDMARLFA
                     LVNVAMADAAISVWKSKYLYDVWRPVTGIRESDEGTGPTGKGDGNPQTQGDPTFWPLG
                     APASNLTGVNFTPPFPSYPSGHAGFGGALFEILRRFYGTD"
     gene            1487..3679
                     /locus_tag="VGE92_02555"
     CDS             1487..3679
                     /locus_tag="VGE92_02555"
                     /inference="COORDINATES: protein motif:HMM:NF016945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylglucosaminidase"
                     /protein_id="HEY1312724.1"
                     /translation="MTHRCACLRLLCLVLLAMSALPGEVRAAFDLKAEHDVLVRLLGD
                     RAAQFDLGAIDAQGGRERFRISNSDARIKVEGTTPSALLFGVNWYLKYIAHVHISTNG
                     SRLGPAGAWPLPTAVIERETPYAYRYALNQNIDGYSAPYWNWARWEREIDVLALSGIN
                     AMIVERGMDTVLYKTFRDVGYSDPEIRGWITQPAHQNWQLMGNLCCFDGPISGRLLAR
                     RAASAQRIIGRLRELGITPVLPGYYGIVPRDFASRFPSAHVIEQGEWAGFTRPGWLDP
                     RDPMFGRLAGAFYRNQRELFGDSSIYDMEVFQEGGDSGDVPVAEAARDVQNALSAAHP
                     GARWMMLAWQGNPRPELLSGVDRSRLFIIDIDHDQVPRDDRLNDFRGAPFLFGGIWEF
                     GGRTTLGANVQNITQRLQLLGRSNGNMVGTALFTEGVDTNPYAFDLFTEMAWRSEPID
                     IRGWTAAYVRRRYGAADSHALAAWQVLLNTAYGIRVDAVKFNSERDAAQESLFDAQPG
                     LSVDRASNWSPQAIRYDAEAFKQALPEMLRVMPRLRSSETYQYDLVDIARQTLANESR
                     LLLPRIKAAFDGKDVSGFRALTAQWLNLMDLQDRLLGANRFFLVGEWLGFVVPWASTP
                     DEAARLNHDARSLLTTWGERKASEGADLHDYGNKDWAGLTRDYYRVRWQTYFASLDAE
                     LESGVPSKPIDWFAMGDAWNRAARIYSRTPEGDVHLIAMQIAEALNLD"
BASE COUNT          725 a         1092 c         1167 g          746 t
ORIGIN      
        1 gatcggtacc gtagaaccga cgcaaaattt cgaacagcgc accaccaaag cctgcgtgcc
       61 cggacggata actcgggaag ggtggtgtga agttgactcc ggtaagattg ctggccggtg
      121 cgcccaaggg ccaaaatgtg ggatccccct gcgtttgcgg attcccatca cctttgccgg
      181 tggggccggt gccctcatcg gactcgcgta tgccggtcac cggtctccat acgtcataga
      241 ggtacttcga cttccacacg gaaatcgccg cgtccgccat cgcgacatta acgagcgcga
      301 ataggcgcgc catgtcgctg acgctcgaac tgctcttgct gatctcggtc gtgatctcat
      361 tgtacaagcg cggcggggcg cacaggctcg gtgtgccgtc ataggcccaa taaatgccga
      421 tgtacgtctg ttcggcggtg cgttgtgtcg gcgtgtggac accgtccccg ccgagcgact
      481 taacttcgtc gtaggcggcg gtatattccg ggctggtgag cgccggaggt ggcgttgcca
      541 ggaactgtat cgctgagcgg aggacgaagg gcttgacctc accccagtag gcccccaaag
      601 caatgggcga gagcgcaatc ggatcttggc gccagtcacc cggttcgttg cccgtaaccc
      661 attcggttcc aagtttcgga tccggcaccg ctgagccatc gtccgctcgc agcgccaaga
      721 tccctgcggc tgcgagatga ccgattgcaa cgccgcgatt cttcatcgca ccgtccctgg
      781 agggaatgcg attgagttct tgcgcataca gcgcgtcgaa gttcggcctt tgcgagggga
      841 atagggcgct caaggtgtcg tgcgcagacg tcgcgatagc ggcatcgacg gatgcaggtt
      901 cgcgggcccg cggcaggccc aagtagcttc ggtagccccc caagattgaa ttgaccgatt
      961 caaacatcga tacatgtacg atcgccattg cacggctcga gcgcgtcgga cccagttgct
     1021 cgccgtagac ccgattctct cccggggcga ccggcgtatg atccaagccg gtcgcatcga
     1081 cggccagctg gttccagtaa cgcagggcat cgacgctggc gttcgagcgt cgatgcgccg
     1141 tgtagcgaaa cacgtccttg gtcgccggct gaacgcgcgg tccatagatt tcctgtggat
     1201 tcttcggcgg cccatccgcc attaccaagc tgctcgaagc gccaaagaaa atagcggcaa
     1261 cgcatatcac agcacggcca actgcggtca cagccatagt aaacctgctc ttataggttg
     1321 ttctaggaac gaatcctaag cgagtctgcg attttcatga aatgtcgcca gctgtgtact
     1381 atttcggcga tgacaatcga caactgtcaa atcaaaggcg cgcgcgcgct tatgcggcag
     1441 gcgcaaggat aaggtatcgt ttgcgcgtgt agcggactac gagttcatga cgcatcgctg
     1501 tgcctgtctg cggctgcttt gtttggtact cctggcgatg tcggcgctgc cgggcgaagt
     1561 gcgcgcagcc ttcgacctca aagccgagca cgatgtgctg gtacgcttat tgggggatcg
     1621 cgcggcgcag ttcgatttgg gcgcgatcga cgcgcaaggc ggccgcgaac gatttcgcat
     1681 ttcgaattcc gatgcacgca tcaaagtgga aggcaccacc ccgagcgcgc tgctgtttgg
     1741 cgtgaattgg tacctgaaat atatcgcgca cgtgcacatt tcaaccaacg ggtcgcggct
     1801 cggcccggca ggtgcgtggc cgttgccgac agccgtcatt gaacgcgaaa caccctacgc
     1861 ttaccgctac gcgctcaatc aaaatatcga cggctattcg gctccgtatt ggaattgggc
     1921 acgctgggag cgagagatcg atgtgctggc gctgtcgggg atcaacgcga tgatcgttga
     1981 gcgtggaatg gataccgtgc tctacaagac gtttcgcgac gtcggctact cggacccgga
     2041 gatccgcggc tggatcacgc aacccgcgca tcagaactgg cagttgatgg ggaacctgtg
     2101 ctgcttcgac gggccgatca gcggccggct gctcgcgagg cgcgccgcct cggcgcagcg
     2161 aattattggg cgcctgcgcg aactcggaat cacgccggtg ctgccgggct actacggaat
     2221 cgtgccgcgc gattttgcaa gcaggtttcc gagcgcccat gtgatcgaac aaggcgaatg
     2281 ggcggggttc acccggcccg gatggctcga tccccgagat ccgatgttcg gccggctggc
     2341 aggcgcgttt taccgcaacc agcgcgaact ctttggcgat agcagcatct acgacatgga
     2401 ggtattccag gagggcggcg attcgggcga cgtgcctgtg gcggaagcag cccgcgatgt
     2461 acagaacgcg ctcagcgctg ctcatcctgg cgcccgctgg atgatgctcg cctggcaggg
     2521 gaatccgcgc ccagagttat tgagcggcgt cgatcgcagc cgccttttca tcatcgatat
     2581 cgaccatgat caggtgcctc gtgatgatcg actcaacgat tttcggggcg cgccgttcct
     2641 attcggcggt atttgggaat ttggcggtcg tacgacgtta ggcgcgaatg tccagaatat
     2701 tacgcagcgg ctgcaactgc tgggaagaag caatggcaac atggttggaa ccgcgctgtt
     2761 caccgagggt gtggacacga acccgtatgc cttcgatctg ttcaccgaga tggcctggcg
     2821 aagcgagccg atcgacatcc gcggatggac cgcggcctac gtgcgccgtc gctatggagc
     2881 cgccgattct catgcgctgg cggcgtggca ggttttgctg aataccgcct atggcatccg
     2941 cgtcgatgcc gtcaagttca acagtgaacg tgatgccgcc caagaatcgc tatttgacgc
     3001 acagccgggt ctcagcgttg atcgagcgtc gaactggtcg ccgcaagcga tccgctacga
     3061 cgccgaggcc tttaaacaag cactgcctga aatgctgcga gtgatgccgc ggctgcgcag
     3121 cagcgaaact tatcagtacg acctcgtgga tatcgcccgc cagacgctgg ccaatgagag
     3181 ccgtctgctt ctgccgcgga tcaaggcggc gttcgacggc aaggacgtat cgggctttcg
     3241 cgccttgacc gcgcaatggt tgaatctcat ggacctacaa gatcggctgc tcggtgcgaa
     3301 ccgctttttt ctggtcggcg aatggctggg gtttgtcgtg ccctgggcgt caacgcccga
     3361 cgaggctgca cggcttaacc acgatgcgcg atccttgctc accacctggg gcgagcgcaa
     3421 ggcgagcgaa ggcgcggatc tccatgacta cggaaacaag gattgggcag gcctcacgcg
     3481 cgactattat cgggttcgct ggcagaccta tttcgcgtcc ctcgacgccg aacttgagag
     3541 cggggtcccc agcaagccca tcgattggtt tgcaatgggt gatgcttgga atcgcgccgc
     3601 gcggatctat tcccgaacgc ccgagggtga cgtccatcta attgcgatgc agatcgcaga
     3661 ggcgctgaat ctcgattgac gaaacgacca tggagccgcg cgctcgtgca gtcgtacaat
     3721 cacgggcagc
//
LOCUS       DASWZT010000098         2676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_44297, whole genome shotgun sequence.
ACCESSION   DASWZT010000098 DASWZT010000000
VERSION     DASWZT010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44297"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VGE92_02560"
     CDS             <1..434
                     /locus_tag="VGE92_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312725.1"
                     /translation="PVDGGLEILTDDGSWAHRVSRGVHALTVDYVLRMRGPLNDDLVA
                     EFRAATDCEGEAVSILEANAQYGEGFNHWLNVTVRATRSAQVRHWWGARGIIVSRLMR
                     VRFGPVHLSRELPRGHSRAMLTSERNALLNEIDAAAKPAVS"
     gene            complement(427..1518)
                     /locus_tag="VGE92_02565"
     CDS             complement(427..1518)
                     /locus_tag="VGE92_02565"
                     /inference="COORDINATES: protein motif:HMM:NF005071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY1312726.1"
                     /translation="MAAARILAACLSAALCWAAHAAAPAAAPIPVEGEIIARNFHFAS
                     GEMLPELRLHYLTLGEPKRDSHGRINNAVLLLHGTGGSSRSLLNEHFAGVLFGHGQLL
                     DSAKYYLILPDAIGHGKSSKPSDGLHARFPQYTYADMVAAQHSLLEGLQVEHLRLIIG
                     TSMGCMHTFMWGETYADFVDALMPLACLPAQIAGRNRLWRNLLMEAIRADPQWLQGEY
                     KSEPPGALRTAAGLLLIAGSAPIQMQLALPSRDAADQFLEKFMQRELDDLDANDLLYQ
                     MNASRDYDPSPALESIKAPLTLVNSADDFINPPELGIAEREIKRIKGARFVLLPASDQ
                     THGHGTHTWAALWQQYLAQLLQASQPAAN"
     gene            complement(1533..2279)
                     /locus_tag="VGE92_02570"
     CDS             complement(1533..2279)
                     /locus_tag="VGE92_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009387635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HEY1312727.1"
                     /translation="MHPKDFSPDRDSLADRVILITGASSGLGRALAIESARCGASVIL
                     CGRNAAKLERVYDEIETLGAPQPAIAMLDLAAATAVDYDQLAKTIDGEFGRLDGAVHA
                     AGLLGERTPLEQYDVPTWCKVLHVNLTAPFILTQVLLPNLRASPDASVIFVSSGVVKN
                     QRPFWGAYAVAKTGLESVRSMFAQELEGEANIRVNSVNPGRMRTAMRAAAYPGEDPNT
                     VPTPAAVCAAFLYLLSAESRGIDDQYFEAQ"
     gene            complement(2298..>2676)
                     /locus_tag="VGE92_02575"
     CDS             complement(2298..>2676)
                     /locus_tag="VGE92_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312728.1"
                     /translation="LRRCCEALAAADYLSRAIRDYRREARAGRVPFAVAELLDAGIGN
                     DDLAAEVPPAPLLRYLEDLRERAAGGFASAARALPQAERRRHRHLLVLAALELDALHG
                     GPRRPRVRRFKDMLLAWNSARRA"
BASE COUNT          476 a          848 c          887 g          465 t
ORIGIN      
        1 ccccggtcga cggcggcttg gagatcctca ccgacgatgg ctcctgggcg caccgtgtgt
       61 cgcgcggcgt gcatgcactc accgtcgatt atgtgctgcg catgcgcggc ccgctcaacg
      121 acgatctggt ggccgaattc agggcggcca ccgattgcga aggcgaggct gtgagcatcc
      181 tcgaggccaa cgcgcagtac ggcgaaggat tcaatcattg gctcaacgtc acggtgcgcg
      241 cgacgcgctc cgcgcaagtg cggcattggt ggggggcacg cggcatcatc gtcagccgcc
      301 tgatgcgcgt gcgtttcgga cccgttcact tatcgcgcga gttgccgcgc ggacactcgc
      361 gcgccatgtt gacgagcgag cgcaacgcgc tgctcaacga gatcgatgcg gccgcaaagc
      421 cggccgtcag ttagcggcag gctgcgaggc ttgcagcaat tgcgccagat actgttgcca
      481 gagggcggcc caggtatgcg tgccatgccc atgagtctga tccgaggccg gcaacagcac
      541 gaagcgcgcg cctttgatgc gcttgatctc gcgctccgcg atcccgagtt ccggcggatt
      601 gatgaaatcg tcggcggagt tgaccagggt tagcggtgct ttgatgcttt cgagggcggg
      661 cgaaggatcg taatcgcgcg atgcgttcat ctggtaaaga aggtcattgg cgtcgagatc
      721 gtcgagttcg cgctgcatga atttttcgag gaattgatcg gcggcatcgc ggctgggcaa
      781 cgccagctgc atttggatcg gcgcgctccc ggcgatcagc agcaggccgg ctgcggtgcg
      841 taacgccccg ggcggttcgc tcttgtattc gccctgcagc cactgcggat cggcgcggat
      901 cgcttccatc aggagattgc gccacaggcg gttgcggccg gcaatctgcg cgggcaggca
      961 ggcgagcggc atcagggcgt cgacgaaatc cgcataggtc tcgccccaca tgaaggtgtg
     1021 catgcagccc atcgaggtgc cgatgatcag ccgtagatgc tcgacctgca ggccttccag
     1081 caaggagtgt tgcgcagcca ccatgtccgc atacgtgtat tgggggaagc gcgcgtgcag
     1141 cccgtcgctt ggcttgctgg actttccgtg accgatggcg tccggaagaa tgaggtagta
     1201 cttggcgctg tcgagcaact gtccatggcc gaacagcacg ccggcgaaat gctcattgag
     1261 caggctgcgc gaagagccgc cggtgccatg cagcagcagc accgcattgt tgatcctgcc
     1321 atgcgaatcg cgtttcggtt cgccgagcgt gaggtaatgc aagcgcaact ccgggagcat
     1381 ctcgcccgat gcaaaatgaa agttgcgcgc gatgatctcg ccctccacgg ggatcggcgc
     1441 cgccgcgggg gcggcggcgt gggcggccca acatagcgct gcgctcagac aagcggccaa
     1501 aattcgagct gcagccatca ccgtcccctt tgtcattgcg cttcgaaata ttgatcatca
     1561 ataccgcgac tttccgcact cagcaagtac aaaaaggcgg cgcaaaccgc cgccggtgtc
     1621 ggcacggtgt tcggatcctc gccagggtat gcggcggcac gcatcgccgt gcgcatacgg
     1681 ccgggattca cgctgttcac gcggatgttc gcttcgccct cgagttcctg agcgaacatg
     1741 ctgcgcaccg attccaagcc ggtcttggcg acggcgtagg cgccccaaaa cggccgctga
     1801 ttcttgacta cgccgctgct cacgaaaatc accgaggcgt ccggcgatgc gcgcaaattc
     1861 ggcaacagca cctgggtcaa gatgaacggc gcggtgaggt tcacgtgcag cactttgcac
     1921 caggtcggca cgtcgtactg ctcgagcggc gtgcgctcgc cgagcaaacc cgcggcgtgc
     1981 accgcgccgt cgagtcgtcc aaactcgccg tcgatggttt tcgccagctg gtcatagtcc
     2041 acggcggtcg cggcggcgag gtcgagcatc gcgatcgccg gctgcggcgc gcccagggtt
     2101 tcgatctcat catagacgcg ctcgagcttg gcggcattgc ggccgcacaa gattacgctg
     2161 gcgccgcagc gtgcgctttc gatcgcgagt gctcggccca aaccgctgct cgcaccggtg
     2221 atcaaaatca cgcgatcggc gagcgaatcg cggtcgggac tgaagtcttt cggatgcatg
     2281 tcgagctccg caaatcgtta ggcgcgacgc gccgaattcc aggcgagcag catatccttg
     2341 aagcggcgca cgcgcggccg ccgcgggccg ccatgcaatg cgtcaagctc cagcgccgcc
     2401 agcaccagca ggtgtcgatg ccggcgtcgc tctgcctggg gcagcgcgcg cgccgcgctc
     2461 gcaaagccac ctgccgcccg ttcacgcagg tcctcgagat agcgcagcaa cggcgccggc
     2521 ggcacttcgg ccgcgagatc gtcattgccg atgccggcgt ccaacagctc ggcaacggcg
     2581 aaaggcacgc gaccggcgcg cgcttcgcgg cgatagtcgc gaatggcccg cgacaagtag
     2641 tcggctgcgg cgagcgcttc gcagcaccgg cgcaag
//
LOCUS       DASWZT010000099         3469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_87699, whole genome shotgun sequence.
ACCESSION   DASWZT010000099 DASWZT010000000
VERSION     DASWZT010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87699"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3469
                     /locus_tag="VGE92_02580"
     CDS             <1..>3469
                     /locus_tag="VGE92_02580"
                     /inference="COORDINATES: protein motif:HMM:NF036484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312729.1"
                     /translation="EILFFGPMAQLATAHNSLTADYLYRRKRMELPASDAGTAAPGAT
                     APGRLLLRGASEHNLKNIDVAIPLRRLVCVTGVSGSGKSTLVHDILFPALLRAKGKPT
                     ESPGCHRALDGADCIDAVVMVDQSRIGRTTRSNPASYVGAFDAIRDLFAKLPQARERK
                     YSAGTFSFNAGNGRCPVCGGNGFEHVEMQFLSDVYLRCPDCDGRRYRAEVLEVRLTRG
                     AAPPKSIADVLELTVSEALRFFAADAEVCLRLAPLADVGLDYLRLGQPVPTLSGGEAQ
                     RLKLAGHLAENAVRRGSTSAASGAGPRLGSLFLFDEPTTGLHFDDVAKLLGAFRRLIA
                     AGHSLLVIEHNLDVVRAADWIIDLGPEGGAAGGQVVCFGTPAEVMRVPASHTGRALLD
                     ADKPPALVAPNAVRDSGVCAYSAPTHIEIRGAREHNLKNVDVNLALNQFTVITGVSGS
                     GKSTLAFDILFAEGQRRYLESLNAYARQFVQPASRPDVDAIFGIPPTVAIEQRTSRGG
                     HKSTVATLTEIYHFLRLLYVKLGVQYCPVCEARIEPQSADAIAARVLKDYRSKSITVL
                     APLIVARKGLYTALAKWARGKGFSHLQVDGALLPTAKWPRLDRFIEHNIELPIAELRV
                     SAGREAELRQALDSALQHGRGVVHVAAAQGGAGASAAVNIFSTKRACPNCGTGFPELD
                     PRLFSFNSKHGWCKSCFGTGLQLPQFGPEQSGEESAWREAQSVTCPDCAGQRLNPVAL
                     HVRFRDRSIAALSDLPVRDFSAALQSLKLIGRERDIARDLTSELITRSTFLCDVGLGY
                     LQLNRAAPTLSGGEAQRIRLAAQLGSNLQGVCYVLDEPTIGLHPRDNVALLDTLDRLR
                     ANGNTLVVVEHDEDTIRRSDHVIDLGPGAGTRGGEVVAQGTAAQLARCGESATGRCLA
                     APLQHSRSRRRRVDNTTPVIEVQGAALHNLRKVDAKFPLGRLTVVTGVSGSGKSSLAR
                     DVLLHNLQLLLSRPRRASRGQQPDAVLEGCAAMSGWENISRVLEVDQTPIGKTPRSCP
                     ATYIGFWDSIRRLYADTTEARIRGFTASRFSFNTAGGRCEACEGQGMQRIEMSFLPDV
                     QVLCDVCGGQRFNPETLAILFKGKSIGEVLSMSVDEALEFFVAQRSVHHPLSLLADVG
                     LGYLSLGQP"
BASE COUNT          563 a         1167 c         1110 g          629 t
ORIGIN      
        1 gagagatatt gttcttcggg cccatggcgc aactcgccac ggcgcacaac tccctgactg
       61 ccgactatct gtatcgccgc aaacgcatgg aactcccggc gtcggacgcg ggcacagcgg
      121 cgcccggcgc aacggcgccc ggacgattgc tgctgcgcgg cgcaagcgag cacaatctca
      181 agaacatcga cgtggcgatt ccgctgcggc gcttggtgtg cgtcaccggc gtcagcggtt
      241 cgggaaaatc cacgctggtt cacgacattc tgttccctgc gctgctgcgc gccaaaggta
      301 agcccaccga gagtccgggc tgccaccggg cactcgacgg tgcggattgc atcgacgcgg
      361 tggtgatggt ggaccagagc cgcatcggcc gtaccacgcg ctccaatcct gccagctatg
      421 tcggcgcctt cgatgcgatt cgcgatttgt tcgccaaatt gccgcaggcg cgcgagcgca
      481 aatactccgc cggtaccttc agcttcaacg ccggcaacgg ccgctgcccg gtgtgcggcg
      541 gcaacggctt cgaacatgtc gagatgcaat ttctctccga cgtgtactta cgatgtcccg
      601 actgtgatgg gcggcgctat cgagccgaag tgctcgaggt gcgtttgacc cgcggcgcgg
      661 cgccgccgaa gagcatcgcc gatgtgctcg agctcaccgt ttccgaagcg ctgcgattct
      721 ttgccgccga tgccgaagtg tgcctgcgct tggcgccgct cgccgacgtc ggtctcgact
      781 acctgcgctt gggtcagccc gtgccgacct tgtcgggcgg cgaggcgcag cgcctgaagc
      841 tcgccggaca tctcgccgag aatgccgtgc gccgggggtc cacgagcgct gcgtccggcg
      901 ccggcccgcg cttgggttcg ctgtttctgt tcgacgaacc gaccaccgga ctgcatttcg
      961 acgacgtcgc gaagttgctc ggcgctttcc ggcgtctcat cgccgccggt cactcgctgc
     1021 tcgtgatcga gcataatttg gacgtggttc gcgccgccga ttggatcatc gacttgggcc
     1081 ccgagggcgg cgcggccggt ggccaggtgg tctgcttcgg cacgccggcc gaggtgatgc
     1141 gcgttcccgc ctcgcatacc ggacgagcgc tgctcgacgc cgacaagccg ccggccttgg
     1201 tggcgccgaa cgctgttcga gattccggcg tttgcgcgta cagcgccccg acccacatcg
     1261 agattcgcgg cgcccgcgag cacaacctca agaacgtcga tgtgaacctg gcgctcaacc
     1321 agttcacggt gatcaccggc gtctccggca gcggcaagag caccctggct ttcgatattc
     1381 tgtttgccga gggccagcgt cgctacctcg agtcgctgaa tgcctacgcg cggcagttcg
     1441 tgcagcccgc atcccggccc gacgtcgatg ccatcttcgg cattccgccg acggttgcca
     1501 tcgagcagcg caccagccgc ggcgggcata agagcacggt ggcaacgctg accgagatct
     1561 atcatttctt gcggctgctc tacgtgaagc tcggcgtgca gtattgcccg gtgtgcgagg
     1621 cgcgcatcga gccgcaatcc gcggatgcca ttgccgcacg cgtgctcaag gactatcgca
     1681 gcaagagcat cacggtgctc gcgccgctga tcgtggcgcg caagggtctg tacactgcgc
     1741 tcgccaagtg ggcgcgcggc aagggcttct ctcacctgca agtcgacggc gcgctgctgc
     1801 cgacggcgaa gtggccgcgg ctcgatcgct tcatcgagca caatatcgag ctaccgattg
     1861 ccgagttgcg cgtaagcgca ggccgcgagg ccgaacttcg ccaggcgctc gattcagcgc
     1921 tacagcacgg ccgcggcgtg gttcatgtgg ccgcggcaca gggcggtgcc ggggcgtccg
     1981 ccgccgtcaa catcttttcg accaagcgcg cctgccccaa ttgcggcacg ggctttccgg
     2041 aactcgaccc gcgcctgttt tccttcaact cgaagcatgg ctggtgcaag agctgcttcg
     2101 gcaccggctt gcagctgccg cagttcgggc ctgagcaaag cggcgaagaa tccgcttggc
     2161 gcgaagcgca atccgtcacc tgtcccgact gcgccggcca gcgcttgaat cccgtggcct
     2221 tgcacgtgag gttccgtgac cgatcgatcg cggcgcttag cgacttgccg gtgcgcgatt
     2281 tctccgccgc gttgcagtcg ctcaaattga tcggccgcga acgcgacatt gcccgcgacc
     2341 tcacgtccga gctcatcaca cgcagtacct tcctgtgcga tgtcggcttg ggttatctgc
     2401 aattgaaccg cgccgcgccg actctgtccg gcggcgaggc gcagcgcatc cgcctggcgg
     2461 cgcagctcgg ctccaacctg caaggcgttt gctatgtgct ggatgaaccg accatcggtc
     2521 tgcacccacg cgacaatgtc gcactcctcg ataccctcga cagactgcgt gccaacggca
     2581 atacgctggt cgtggtcgaa cacgacgaag acaccattcg ccgctccgat catgtgatcg
     2641 acttgggtcc gggcgcgggc acgcgcggcg gtgaggtcgt ggcgcaaggg actgccgcac
     2701 aactggcccg ctgcggcgaa tccgcgaccg gccgctgcct ggcggcgccg ctgcagcact
     2761 cgcgctcgcg ccgccgccgc gtcgataaca ctacgccggt gatcgaagtg cagggcgcgg
     2821 ccttgcacaa tctgcgcaaa gtcgacgcga aatttccgct cgggcgattg accgtggtca
     2881 ccggcgtctc cggatccggt aagtcctcgc tggctcgcga cgtgctgttg cacaacttgc
     2941 agctgttgtt gtcgaggcca cgccgcgctt cccgcgggca gcaacccgac gcggtgctcg
     3001 agggctgtgc cgcaatgagc ggatgggaga acatcagccg cgtgctcgag gtcgatcaaa
     3061 ctcccatcgg caagacgccc cgctcctgcc cggccaccta catcggattc tgggactcca
     3121 tccggcgcct gtatgccgat accaccgagg cgcgcatacg cggcttcacc gccagccgct
     3181 tttcgttcaa tacggcgggc ggccgctgcg aagcctgcga gggccaggga atgcagcgca
     3241 tcgagatgag tttcctgccc gatgtgcagg tgttatgcga tgtgtgcggc gggcaacgct
     3301 tcaatcccga gaccctcgcg atactgttca agggcaagag catcggcgag gtgttgtcga
     3361 tgagcgtcga cgaagcactg gagtttttcg tcgcgcagag aagcgtccac catccgctga
     3421 gcctgctcgc ggacgtcgga ctcggctact tgagcctcgg ccagccgag
//
LOCUS       DASWZT010000100        11260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_73279, whole genome shotgun sequence.
ACCESSION   DASWZT010000100 DASWZT010000000
VERSION     DASWZT010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11260
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73279"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1688)
                     /locus_tag="VGE92_02585"
     CDS             complement(<1..1688)
                     /locus_tag="VGE92_02585"
                     /inference="COORDINATES: protein motif:HMM:NF015118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="HEY1312730.1"
                     /translation="MLSLGNASAIRREITAAQRIPYAAHVAPSVISTVFGDYVQVLRL
                     GGASFETSDDATLNNWHERLNVLWRNIASPNAALWSHVIRRRTGIDSASRESGPRQPG
                     DELFSSMLDAKYRGRLAHETLMVNELYLAVVHRPAPGVAAGVLSKLLAKSRPREAQGI
                     RADALDACEKLAQTLTASLARYEPERLGVYQSGSLWCSSLLEYLGLLINGEWQRAALP
                     CGPLNQALATTRLFFGSEAIEFRSPTASRVGAMLGIKEYPTPSVVGMYGRLLSAPFGF
                     VLTQSFTFLTKATGQSLLQRQFNRMANAGDFAVSQALELKDALDALTSNEFVMGDHHF
                     SLQLHAELPQAAADGAGGERLKALNDHIGLARSYLADTGMLVAREDLALEAAFWAQLP
                     GNFPARPRKAPITSRNFAAMAPFHNYPLGRAHGNHWGDALSMFVTRARSPYYFSLHAS
                     DPKDPDGGSRKDTGHTLICGPTGSGKTVFIGFLLAMLDAQGATQVVFDKDRGLEILVR
                     AMGGDYLPLKSGVATGFNPLQLPVTPANVEFLKLWLGELSAASPAARARSARETA"
     gene            1951..2196
                     /locus_tag="VGE92_02590"
     CDS             1951..2196
                     /locus_tag="VGE92_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312731.1"
                     /translation="MLAFRVEIDGELAHVAGVADWSLLAMHITASRGDATAPVESAMQ
                     HDSLQALQLATDLESVLKRLSNKWDFYYPTGEGYFDE"
     gene            2408..2869
                     /locus_tag="VGE92_02595"
     CDS             2408..2869
                     /locus_tag="VGE92_02595"
                     /inference="COORDINATES: protein motif:HMM:NF025196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldoxime dehydratase family protein"
                     /protein_id="HEY1312732.1"
                     /translation="MVVIYLGMRVNALTGLKTLLGLGPQINKAGAARPPGLLHFENKI
                     IYSLFPFHVGMRWYWRDFESMENWTRSPPHKVWWQSFLKDSGGTGFWHEAYFVGGGME
                     AIYSDVETPLGFLAFAPVVAARGSAFSARRRAGHQGAVPSQPEGASESEIY"
     gene            3436..4347
                     /locus_tag="VGE92_02600"
     CDS             3436..4347
                     /locus_tag="VGE92_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312733.1"
                     /translation="MASRAYLLSLPERVIRSALGLSAGLLREVGVVVLPQGVRRSALY
                     RNLVDTTLRYVIEHVGGAEGVYPAEQPQPDNFLTRRGAGHAIELLGIVAFRVSPVWVL
                     AALADLSGLGRRLIPEIAEALKAEGLLHEGSQFESVDQLLDGLEKTSLRLARALYVPP
                     LNVAELRKEWQTIRGQARGANPLQLPSPTAIGKQWEQLTQESTRQGRSIFETSSVMAI
                     AAVRALPNRTRWLSASTRVGARSAGRLLAADVLDGYSQTLNELREVGYLTYAKRQLSP
                     YLRACVDQFSQQRPTLTQRLLARTRKN"
     gene            4465..5529
                     /locus_tag="VGE92_02605"
     CDS             4465..5529
                     /locus_tag="VGE92_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY1312734.1"
                     /translation="MRITQLFVLIGAIGSAWLLSSCATTSPTSAAAETARITVLYDAF
                     GKDAAMTKDWGYAALVEINGKRVLFDTGDDPAIFAKNVKAKGVDLTKLDFLVLSHRHS
                     DHVSGISYLLSVNPKVKIYAPKEGFGIFGSDLPSKFYRKDEVLPAEMRYYGGRPPEIL
                     KFGTVFPGANIQLIDKTTELIPGITLIALVSDAPGTKELKELSLAINTPNGIILVVGC
                     SHPGIESIVAEAAKINPHIHFIAGGFHLVVAQDPAIEKVATTLHDTYQVDYIAPGHCT
                     GEPTFAALQRTFGDHYLYAGLGTTLDLGANPRAASDRHAIGVLDESDLRTYRTLLAQS
                     ANLSARDAKDRNGSNPVAGP"
     gene            5780..6499
                     /locus_tag="VGE92_02610"
     CDS             5780..6499
                     /locus_tag="VGE92_02610"
                     /inference="COORDINATES: protein motif:HMM:NF039346.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312735.1"
                     /translation="MQTNTHEIADRIFRLSTCVPEAAPGGFTFNQFLVDADDPLLFHT
                     GPRRMFPLVSQAIARIIPLKRLRWITFGHVESDECGAMNEFLAAAPSAEVAHGAIGCM
                     VSLDDLCDRPPRKLADGEVIDLGGKQVRHIDTPHVPHNWEARVIFEQTTKTLFCGDLF
                     THTGDGPALTDDDIVGPALAAEQMFHATALSGATASTIRRLAALEPQMLALMHGSSAR
                     TRCGDSLLRLADAYDSMSKQG"
     gene            6870..7256
                     /locus_tag="VGE92_02615"
     CDS             6870..7256
                     /locus_tag="VGE92_02615"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY1312736.1"
                     /translation="MLDHIGLVVSNLQKSRQFYLVALAPLSIGIVMEVTKEQTGTYAG
                     AGFGASNKPSFWIGTGDHLSGPVHIAFTASSREDVDAFYKVALSVGGRDNGAPGVRAH
                     YHPNYYGAFVLDPDGNNVEAVCHGPA"
     gene            7715..8539
                     /locus_tag="VGE92_02620"
     CDS             7715..8539
                     /locus_tag="VGE92_02620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY1312737.1"
                     /translation="MSSIRAVAGLPLVRTIVYHPAVRGRLGRLPGFGRFYGSGWDFRH
                     PFDRRYHTETSGSMPSDQIQSSPYDSDKVYIYAGSQPSIIRSALNTLPALSGCAFVDL
                     GCGKGRPLLVASEYPFRSIVGVELTPSLAEAARRNAAIINRQFAQRTPIQIEQQDAGQ
                     FVPPAGNAVVFLYNPFGEEVIAKVVSRIEEALAAGGRSIFVIYYNPVHAHCFDASRAL
                     RRYFAANLPYSPEELGYGPEITDAVIIWQGGGAAPARGNADATIKIIWPGVRAELA"
     gene            complement(8499..8903)
                     /locus_tag="VGE92_02625"
     CDS             complement(8499..8903)
                     /locus_tag="VGE92_02625"
                     /inference="COORDINATES: protein motif:HMM:NF016957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HEY1312738.1"
                     /translation="MSAANSGLLADPLFVGATRPPMRWGVTYSALLFNMVLTMEVFLL
                     TKNLLTLLLCLPVHAICMSLCARDARYFDLVLLWARTRFPAMFANLRYWKASSYGALS
                     LNLPLHGRRRPEISDVRLQPMRVLPARPARLS"
     gene            complement(8913..9200)
                     /locus_tag="VGE92_02630"
     CDS             complement(8913..9200)
                     /locus_tag="VGE92_02630"
                     /inference="COORDINATES: protein motif:HMM:NF016820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HEY1312739.1"
                     /translation="MHLPRSHRSTLIIILLGAPGLALAQASPFMTGATALQTNLLAWL
                     TPVAIILVMVLGGMAMANRMSWGWCIGAILGIAIGFGAPQIVSWVRGMFGV"
     gene            complement(9218..9613)
                     /locus_tag="VGE92_02635"
     CDS             complement(9218..9613)
                     /locus_tag="VGE92_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY1312740.1"
                     /translation="MNSFTVTAVGNLANDPELAIKGDITYTKVCLIGNDYAGKDAHGN
                     PREAATSVYFVAFENLGEVIAKNARKGDQLIVQAQIRSNNWTDKDGEKRYDYSFVIQN
                     FRFGAPGRIKREELAALRDATEGALETGT"
     gene            complement(9734..10453)
                     /locus_tag="VGE92_02640"
     CDS             complement(9734..10453)
                     /locus_tag="VGE92_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312741.1"
                     /translation="MSSDGSALMTHIHLVGGEKGGVGKSLLARLLAQYFIDNSIAFIG
                     FDSDRTHGALLRFYAGYASAIAMDNYESLDAIVEAASAVPERRILVDLAAQTNHGLTQ
                     WMDESQLLEVAPEIGLNIRYWHVMDAGRDSVDLLKRLLDRHGTRLNYTLVLNQVRGAD
                     FRILDGSGEKQRAQALGASIVTLRRLHETSMIKIDAGSMSFWAVANNSEADTSGLTLL
                     ERQRVKVWLRGAYEEIAKVGV"
     gene            10498..>11260
                     /locus_tag="VGE92_02645"
     CDS             10498..>11260
                     /locus_tag="VGE92_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1312742.1"
                     /translation="MPTAALHNSPEPAALRATRLRKVYGSVTALDDVSFTVKRGEIVG
                     LLGPNGAGKTTTINMVLGVLAATTGSIEIGGIDIAKQRAKALARTNFAAVYAALPGNL
                     TVLQNLRFFGLLYAIRGLQARAEELLREFTLEHLRDTKCGLLSSGEQTRAALAKALLN
                     RPQLLLLDEPTASLDPSAAQLIRAKIKSLATDGNCGILWTSHNMYEVEAVCDRVLFLS
                     RGKVLLEGDPRSLPHEHGAASLEDLFIRVAREPLAA"
BASE COUNT         2281 a         3386 c         3390 g         2203 t
ORIGIN      
        1 gcggtctcac gggccgagcg agcgcgcgcg gcgggcgatg ccgcgctcag ttctcccagc
       61 cacagtttga gaaattcgac atttgccgga gtgaccggca gttgtaaggg attgaatccg
      121 gtcgcgacgc cgctcttcaa aggcagatag tcgccgccca tggcgcgcac caggatttcc
      181 agcccgcgat ctttatcgaa cacgacttga gtcgcgcctt gggcatccaa catggccagc
      241 agaaatccaa tgaataccgt cttgccggag ccggtgggcc cgcagatcag cgtgtgtccg
      301 gtatccttgc ggctgccgcc atcgggatcc ttgggatcgc tggcatgcag tgagaaataa
      361 taaggagagc gcgcacgcgt gacgaacatg ctgagcgcat cgccccaatg attgccgtgc
      421 gcacgcccga gggggtagtt gtgaaacggc gccatcgcgg caaagtttcg cgacgtgatc
      481 ggcgccttgc gcggccgcgc gggaaaattg cccggcagct gcgcccagaa cgccgcctcc
      541 agggccaggt cttcgcgcgc aaccagcatg ccggtgtccg ccaggtaact gcgcgccagc
      601 ccgatgtgat cgttgagcgc tttcagccgc tcgccgccgg cgccatcggc cgcggcttgc
      661 ggcagctcgg cgtgcagctg taacgagaaa tgatgatcgc ccatgacaaa ctcattgctg
      721 gtgagcgcat ccaaggcatc cttgagttcg agcgcttgcg agacggcgaa gtcgccggcg
      781 ttcgccatgc ggttgaattg acgctgcagc agggattggc ccgtcgcttt tgtcaagaaa
      841 gtgaaggact gcgtcagtac gaagccaaac ggcgccgaca gcagacggcc gtacataccc
      901 acgacgctcg gcgtcgggta ttccttgatg ccgagcattg caccgacgcg gctcgccgtg
      961 ggcgagcgga attcgatcgc ctccgagccg aagaacagcc gcgtggtggc aagtgcctga
     1021 ttgagcggac cgcagggcag cgctgcgcgt tgccattcac cgttgatcaa gagcccgagg
     1081 tactcgagca acgaagagca ccagagactg cccgattgat acacgcccag ccgctcaggc
     1141 tcgtagcgcg cgagagaagc ggtcagcgtc tgtgccagtt tctcgcaggc atcgagcgcg
     1201 tcggcgcgaa tcccctgcgc ttcccgaggt cgcgacttgg cgagcagctt tgacagcacg
     1261 ccagcggcga cgccaggcgc gggtcgatgc acgacggcga gatataactc gttcaccatc
     1321 agcgtctcat gcgccaaccg gccgcgatac ttggcatcga gcatcgagga gaacagctcg
     1381 tcgccgggct gccgcggccc actctcgcgc gacgccgaat caattcccgt gcgtcggcgg
     1441 atcacgtggc tccacagtgc cgcgttggga ctggcgatgt tgcgccagag tacgttgagc
     1501 cgctcgtgcc aattgttgag cgtcgcgtca tcgctggttt cgaaactcgc gccgccgagc
     1561 cgcaacacct gtacgtagtc gccgaacacc gtgctgatca ccgaaggcgc gacgtgagcg
     1621 gcgtaaggaa tgcgctgcgc ggcggtaatc tcccggcgga tggcagaagc gtttcccaac
     1681 gacaacatga cggaggctcc ctcaatgcgc agcggcgaac tgctcggcgc ttgcgggcag
     1741 tgtgagtgac gcttggtcga agctgaaggc gattaagtgc gcgctttagg cagaaacacc
     1801 gtcgaagaaa tccatgtgca cttggccgcc ggcgatgggc agcaagcggc cggtccgtgg
     1861 acacactgaa atcggtgccg cttagcggtc atcggtagct tacggtagtc taacagctga
     1921 gtgccaaatc gaagttcatg aggtgtctgg atgcttgcgt tcagggtcga gatcgatggc
     1981 gagctagctc acgtcgcggg ggtagcagac tggtcactcc tcgcaatgca tatcacggcg
     2041 tcacgtggtg acgcgacggc accggttgaa agcgctatgc agcatgattc acttcaagct
     2101 ctgcaactcg ccaccgacct tgaatctgtc ctaaaacgtc tcagtaacaa gtgggatttc
     2161 tactatccca ccggcgaggg ctattttgac gaataggcgt acttccaacg agcggacgtt
     2221 cgcgacgggc agcttcgggg tcgacaagcg accgttgacc gccctcttgg atatagacgc
     2281 cgccgtgttg ctttgagaaa aggaacggga atagatttgg tgatacttcg aatccgaata
     2341 aatgggagca atgaaaatga agacttccgt caaacggcaa tccgtcgacc ttactggtta
     2401 tccatagttg gtcgtcattt acctggggat gcgggtaaat gccctcactg gtctaaaaac
     2461 gcttttggga ttgggaccgc agatcaacaa agccggcgcg gcccggccgc ccgggctact
     2521 gcactttgaa aacaagatca tctatagtct gtttccattc cacgttggga tgcgttggta
     2581 ttggcgagac tttgaatcga tggagaactg gacccgatcg cccccgcaca aagtctggtg
     2641 gcaatcgttc ttgaaggatt ccggcggcac gggcttttgg cacgaggcct atttcgttgg
     2701 aggcggcatg gaggccatct actccgacgt ggagacaccg cttggctttc tggcgtttgc
     2761 cccggtggtt gctgcacggg gatccgcgtt ttccgcacgt cgccgagccg gacaccaggg
     2821 agcggtacca tcccagcccg aaggagcgtc ggagtccgaa atctattgag gcgcctgctc
     2881 gcttgcagat cggcaacgac ttcacactgc caatcgacgc aagtaaacgc caccgtagac
     2941 aatccgaatg cagtcagccg tcagaggcag tacaggggtc gacagcgccc gcacgtaaga
     3001 aggtaccgag aatgagcgaa cgaggccctc ggcgcatact ggccgacgac cgcaaaaaac
     3061 tcctgcgacc gctgcctttt actgatcgac gagggcaaca catgaccgct caacttaacc
     3121 acatcatcgt ctactcggca gaccgggcga agtccgcgcg ttttctggcc gagatactag
     3181 ggctgcgcgc gccgcgggtt ggggaaatga cgcgatattg gcggctctct cggatccgac
     3241 gtctcggcac gcgaagattt ttcgttcgcc cctaatcacg cgggttcgtg cggccaaggc
     3301 atctgtggca gcgccaccga ttcccgctgc ggcgccgcca agctccgtgc ctaagcagtc
     3361 gacgagcaag gtcggatccg gacgccgcct gctccggccg tgcttgcatg agcaggctgc
     3421 cttacactga gaaacatggc ttcacgggcg tacctactgt cgcttcccga acgcgtgatc
     3481 cgctctgcgc tcggcctcag tgcgggtctg ttgcgtgaag tcggcgtggt ggtgctgccg
     3541 caaggtgtgc gccgcagcgc gttgtacagg aacctcgttg atacaacgct gcgctatgtg
     3601 atcgaacatg tgggtggagc ggaaggtgtt tatcccgccg aacagccgca gcccgataac
     3661 ttccttacta ggcgcggagc cggtcacgca atagagctcc tcggcatcgt cgcgtttcga
     3721 gtatcgcccg tatgggtact cgctgcgctc gcggatctca gcggtctggg gcggcgtctg
     3781 attccggaaa ttgcggaggc gctcaaggcg gagggcctcc tgcacgaagg gtctcagttc
     3841 gagagcgtag atcagctgtt ggacgggctc gaaaaaacct ccttgcgtct cgctcgagct
     3901 ttgtacgtcc cgccgctgaa cgtcgcagaa ttgcgcaaag agtggcagac gattcgcggg
     3961 caggcgcggg gggccaaccc gctacagctt ccgtctccga ctgcgatcgg caaacagtgg
     4021 gagcagctca cgcaggagtc gacgcgtcag gggcgttcga tcttcgagac ctcttcagta
     4081 atggcaatcg ctgccgtacg cgccctgccc aataggacac gttggctgtc agcgtcaaca
     4141 cgcgtcggtg cgaggagcgc cggaaggctc ctggcggcag atgtgctgga tggctacagt
     4201 cagacactga acgagttacg cgaggtgggt tatctcacct atgcgaagcg gcagttgagc
     4261 ccttacttgc gcgcctgtgt tgatcagttc tctcagcagc ggccgacgct tacgcaacgg
     4321 ctactggcgc ggacgcgcaa gaactaatgc gcgtggacgg caactcgcgg cccaagtgcg
     4381 gtcactggac gtcccggttt gacttagctg acgatccagg cttcagcggt gtacgattcg
     4441 atcgcaacaa tcaaggagag agtcatgcga ataactcagc tctttgtcct cataggcgct
     4501 attggctcgg cctggctact ctcgagctgc gcgacgactt ccccgacatc ggcggcagcc
     4561 gaaacagcgc gcatcacagt actctacgat gcctttggca aggatgcggc gatgaccaaa
     4621 gattggggtt acgccgccct ggtggagata aacggaaagc gcgttctgtt cgataccggg
     4681 gatgacccgg cgattttcgc gaagaacgtt aaggccaagg gcgttgattt gaccaagctt
     4741 gacttcttgg tgttatcgca ccgccacagc gatcacgtca gtgggatttc ctatctgctc
     4801 agcgtgaacc cgaaggttaa gatctacgct cccaaggaag gctttggcat ttttggctcc
     4861 gacctaccca gcaagttcta tcgcaaagac gaggtactgc ccgccgagat gcgctattac
     4921 ggcggcagac cgcctgagat tcttaaattc ggcacggtct ttcccggcgc caacattcaa
     4981 ttgatcgaca agaccacgga gctaataccc ggcattacgt tgattgctct ggtgtcagat
     5041 gctcccggaa cgaaagaatt gaaggaattg tcgttggcca tcaacacgcc caacggcatc
     5101 atactggtgg tcggctgttc gcatccgggc attgagagca tcgttgccga agccgccaag
     5161 atcaatccgc acatccactt catcgcgggc ggatttcatc tggtggtggc tcaggaccct
     5221 gctatcgaga aagttgccac aacgctgcat gacacctacc aagtggacta catcgctccc
     5281 ggtcactgta ccggggaacc aactttcgct gcacttcaaa ggacgtttgg cgatcattac
     5341 ctttacgccg ggttggggac gacactggat ctgggcgcga acccgcgtgc cgcatcggat
     5401 cgacacgcca tcggtgtcct ggacgaaagc gacttgcgca cataccgcac cctactcgcc
     5461 caaagcgcta atttgtctgc gcgagacgcc aaggacagga acggatcaaa cccagtcgcc
     5521 gggccttaaa cactttgtca tttccacgcg cagacgaaac gcattacgag agcaggctcc
     5581 gggcgtggcg cgtaaactgc tttttgaatc ggatgatcgc aacgatcaca gctgcgtctc
     5641 tattggtcgc gtgcgcacac gagcgagttg gcactcaact gtcatcgata ggcaacgttg
     5701 atggcgacgc ggcggttctg cacgctgtta agcagctttc gctgggtgcg cgctgacgca
     5761 acaaaaggga aaatgcccca tgcaaactaa tactcatgag atagccgatc gcatcttccg
     5821 cttgtcaacg tgcgtccctg aggcagcgcc cggcggcttc acgttcaatc agtttctcgt
     5881 cgacgcggac gatccgctcc tctttcacac tggcccgcga cgcatgttcc ccctcgtgtc
     5941 gcaggcgatt gcccgtatca ttccgcttaa gcggctccgc tggatcacct ttggacacgt
     6001 ggaatccgac gaatgcggtg caatgaacga attcctcgcc gccgcaccaa gcgccgaagt
     6061 cgcacacggt gccatcggct gtatggtttc gcttgacgac ttgtgcgatc ggccgccgcg
     6121 caagctggcc gacggcgagg tcattgatct cggtggcaag caggttcggc acatcgatac
     6181 gccacacgtc ccgcacaact gggaagcgcg ggtcattttt gagcaaacca ccaagacgct
     6241 tttttgtggc gacctgttta cccacaccgg cgacggaccg gcgcttactg acgatgatat
     6301 tgttgggccc gcgctggctg cggagcagat gtttcacgcc actgcgcttt cgggcgcaac
     6361 cgcatcgacg atccgccgtc ttgccgccct cgaaccgcag atgctggcgc tgatgcacgg
     6421 ctcttcggcg cggacccggt gcggcgattc tcttctcaga ctcgccgatg cctacgactc
     6481 gatgagcaag caaggctgaa cggagagcac aacatcggcg agtaatagaa cccgccagac
     6541 agtcaattag gctgtccgac agcaaacggt ggcggccggc ggcgctcggg ctcgagggca
     6601 gtcatctagg ggtcgcaatt tcctgctgcc aagcggacga ttgcagcttt tgattattct
     6661 tctccgaagc tgacactcac cactgtctcg ttcgggtccg gcattaaaat gaaggtcgtc
     6721 atttgcctgg gatgcgggta aatgccctca ccggtctaaa aacgcttttg ggatttggac
     6781 cgcagagtga gcgaccggtc acacgacgtg acagcaatta tggattggga tcaattggga
     6841 tcgtctaata accttgtgga gagacattga tgcttgatca catcggactg gtagtttcga
     6901 atctccaaaa atcccgtcag ttctacctgg ttgcactcgc tccgttgtct atcggtatcg
     6961 tcatggaggt gacgaaggaa caaaccggca cgtacgccgg ggccggcttt ggtgcgagca
     7021 acaaaccgtc attctggatc ggcacagggg atcatctgag cggaccggtg cacattgcat
     7081 tcacagcatc ctctcgcgag gatgtagacg cgttctacaa agtcgcccta tcggttggcg
     7141 gccgcgataa cggagcacct ggcgtgcgcg cgcactacca cccgaactac tatggtgcat
     7201 ttgttctgga tccggatggg aacaatgttg aggcggtttg tcatggtccc gcgtagccga
     7261 aagcgctaaa catgctcgaa cggcggcttg ggatcggcaa attcgcctcg agacgcgtac
     7321 ttgaaaccgg cgcctgcgca gacctcgggg agccccacgt ccgcaacgag agcctggtga
     7381 acgctgatcg ccatcgatct ctttgcgaat gccgtgagtg ctctgagcgc gagccttcgc
     7441 ccgaatccca agtgagacag gtcgggccag attgggcgcg acctgcgaac cgctgcgaac
     7501 cgacggctca cctgttcgac gaagctcgcg cgatccgatg tgggtacttc gaacgccgac
     7561 gaattgctcc aaccgaactc ttgaatgagc acgccggagt catagaggcc ggcggccgag
     7621 caagcataag cggagcggcc gtgagccgca aaaaatgcgg cggcgttttc ctcaaggtaa
     7681 gatgcgcatc catatgtcgc cgaagaacgt cttgatgtcg agcatccgcg ccgtggcagg
     7741 cttgccgctc gtcagaacga tcgtctatca ccccgcggtg cgcgggcgac ttgggcgttt
     7801 gccgggattc ggtcgattct atggcagcgg ctgggatttc cggcatccat tcgaccgccg
     7861 ctaccacacc gagacgagcg gctcgatgcc ctcggatcaa attcagagca gcccgtacga
     7921 ctccgacaag gtgtacatct atgcgggatc gcaacccagc atcatacgct ccgcattgaa
     7981 cactttgccg gcgctgtccg ggtgcgcttt tgtcgacctg ggctgcggca aggggcgccc
     8041 gttgctggtt gcgtcggaat atccgtttcg cagcatcgtc ggcgtcgagc tgacaccgtc
     8101 attggccgag gccgcgcgcc gcaacgcggc gatcatcaat cgccagttcg cgcagcgcac
     8161 gccgatccag atcgaacaac aagatgccgg ccaatttgtg ccgccggccg gaaacgccgt
     8221 tgtcttcctc tacaacccgt tcggcgaaga ggtgatcgcg aaggtcgtct cgcggatcga
     8281 agaggcgttg gccgccggcg gccgatcgat attcgtgatt tactataatc ccgtacacgc
     8341 gcattgcttc gatgcctcgc gggcgctgcg ccggtatttc gcggcgaacc tgccttattc
     8401 gccggaagaa ctcggctacg gtcccgaaat cacggacgcc gtcatcattt ggcagggagg
     8461 cggcgcggca ccggcacgcg gcaacgccga cgccacgatc aagataatct ggccgggcgt
     8521 gcgggcagaa ctcgcatagg ctgcaggcgg acgtcggata tctcggggcg gcggcggccg
     8581 tgcaacggca gattgaggct caacgcgccg tagctgctcg ccttccaata tcgcagattg
     8641 gcgaacatcg ccggaaatcg cgtgcgtgcc cacaacagca ccaggtcgaa atagcgcgcg
     8701 tcgcgcgcgc acagggacat gcaaattgcg tgaaccggca ggcacagcag cagcgtcagc
     8761 agattcttcg tcaacaggaa cacttccatg gtcagcacca tgttgaagag cagcgcggaa
     8821 taagtgactc cccaacgcat cggcggacgg gtcgctccca cgaacaaggg gtccgcgagc
     8881 agccccgagt tggcagcgct caccgcgcct tcctagaccc cgaacatgcc gcgaacccag
     8941 ctgacgattt gcggcgctcc aaaaccgatc gcgattccca gaattgcgcc gatgcaccag
     9001 ccccaggaca tgcggttcgc cattgccatc ccgccgagca ccatcacgag aatgatggcg
     9061 accggcgtca gccaggcgag aagattggtc tgcaacgcgg tggcgcccgt catgaacggc
     9121 gaggcctgcg ccagcgccag accgggcgcg ccgagcagga taattatcaa tgtcgatcga
     9181 tgcgaacggg gcaggtgcac ggcggaattt ccttttccta agttccagtc tcgagagctc
     9241 cctcggtcgc atcgcgcagc gccgccagct cctcgcgctt gattctgccc ggcgcgccga
     9301 aacgaaagtt ttggatgacg aatgaatagt cgtagcgctt ctcgccgtct ttatcggtcc
     9361 agttgttgga acggatctgc gcctgcacga tgagctggtc gcccttgcgc gcgttttttg
     9421 cgatcacctc gccgaggttt tcgaaggcca cgaaataaac gctggtcgcc gcctcgcgcg
     9481 ggttgccgtg cgcgtccttg ccggcgtagt cgtttccgat caggcaaacc ttcgtgtatg
     9541 tgatgtcgcc tttgatggca agttcgggat cattcgcgag attgccgacg gcggtcactg
     9601 tgaaactatt catggctgct ccttgtctct ggtgtgcgcg actcgggatc tgtatccgtc
     9661 gcgtggcaga gtctaggagc ggcagcgcgg gagctaaatg cgattaatta tcgagttcgc
     9721 gcagaaacaa acgctacacg cccaccttgg cgatctcctc gtacgcgccc ctgagccaaa
     9781 ccttgactcg ctgacgctcg agcagagtca aaccgctagt gtcggcttcg ctgttgttgg
     9841 cgaccgccca gaaactcatg ctgccggcat cgattttgat catcgaggtc tcgtgcagcc
     9901 gcctcaatgt gacgatactc gcgcccaagg cctgcgcccg ctgcttctcc ccggagccgt
     9961 cgaggatgcg gaaatcggcg ccgcgcacct ggttcaagac caaggtgtaa ttcaaacgcg
    10021 taccgtgccg atccaggagg cgcttcagca aatcgaccga atctcgacct gcatccatga
    10081 catgccagta gcggatattg agtccgatct ccggggcgac ttcgagcaat tgcgattcat
    10141 ccatccactg cgtcagtccg tgattggttt gcgccgccaa atccaccaaa atcctgcgct
    10201 ccggaacagc gctcgccgcc tcgacgatcg catcgaggct ttcgtagtta tccatcgcga
    10261 tggccgaggc gtagccggca tagaagcgca acagcgcgcc atgcgtgcgg tccgaatcga
    10321 agccgatgaa cgcgattgaa ttatcgatga aatattgcgc gagcaggcgt gcgagcagcg
    10381 atttaccgac gccgcccttt tcgccgccga ccaaatgaat atgcgtcatc aatgctgaac
    10441 cgtcgctgct cacagtaatt tcctaatcta tcatggtcat ccggcataat cgcggccatg
    10501 ccgaccgctg cattacataa ctcgcccgag cccgccgcgc ttcgcgccac gcggctgcgc
    10561 aaggtgtacg gcagcgtaac cgcgctcgat gacgtatcgt ttacggtgaa gcgcggcgag
    10621 atcgtcggtc tcctcggccc gaacggcgcc ggcaagacca ccacgataaa catggtgttg
    10681 ggagtcctgg ccgcgaccac cggcagcatc gagatcggcg gaatcgatat tgccaagcag
    10741 cgcgcgaaag cgctggcgcg aaccaatttc gccgccgtgt acgccgcgtt gccgggcaac
    10801 ctcacggtgc tgcagaactt gcgattcttc gggctgttgt acgccatccg aggattgcag
    10861 gctcgcgccg aggaattgct gcgcgaattc actctcgagc atctgcgcga taccaaatgc
    10921 gggctgctgt cctcgggcga acagacgcgg gcggcgctcg ccaaggcctt gctcaatcgc
    10981 ccgcaactgc tgttgctcga tgagccgacc gcctcgctcg acccgtcggc ggcgcagctg
    11041 attcgtgcga aaatcaagag cctcgcgacc gacgggaact gcggcattct gtggacttcg
    11101 cacaatatgt acgaagtcga agcggtctgc gatcgcgtgc tgttcttgtc gcgcggcaag
    11161 gtcctgctcg aaggcgaccc gcgtagcctg ccgcacgagc acggcgccgc gagcttggag
    11221 gatcttttta tccgcgtggc gcgcgagccg ctcgcggcag
//
LOCUS       DASWZT010000101         3438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_5495, whole genome shotgun sequence.
ACCESSION   DASWZT010000101 DASWZT010000000
VERSION     DASWZT010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5495"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VGE92_02650"
     CDS             complement(<1..831)
                     /locus_tag="VGE92_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY1312743.1"
                     /translation="MSARPQDGAVSAATPNVRPQLQAALDKQRQAYTRDPVPTYRQRV
                     ADLRTLRRFLLENRDPLIEAVNADYGNRSRHETLFTELVPVLDGTKHALKNLRAWMRP
                     QRRSVDLLLFPGARNRLRPQPLGIVGIIVPWNFPINLAFSPLTSIFAAGNRAMIKLSE
                     NSRHLAALLIERLPRYFPADKLAVFDETGGVGIEFSRLQLDHLLFTGSTQTGRSVMSA
                     AAGNLVPVTLELGGKSPALLCEGFPLRTAAERILFVKLLNAGQICTTIDYLWMPRARI
                     D"
     gene            complement(851..3250)
                     /locus_tag="VGE92_02655"
     CDS             complement(851..3250)
                     /locus_tag="VGE92_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1312744.1"
                     /translation="MFTRRRSIYLSVLALCAGSGGAVAADATSEQASMPAAEGELATI
                     TITAQRRTEASQDVPIALQVVTSQQIEKLAATDLATMNGYVPGLDVSGEQPTQPGFTL
                     RGISVSDFGIGTDSPIGLYEDGVYTGKTGGALLLFNDVQRVEVLKGPQGTLFGRNSAA
                     GAISVITNEPTDTWAGDAKARFGNFGEKYYEAMLNAPLGPDLAARVTFVDNHSDGWLQ
                     DSATGARYDRNGDWGTRAQLLWRAPGDTRVRLIWEHEDLDQPARPAIGIATLPPSPGL
                     PPFPSTPNTWISPFNAPVLNDVIDGRETRKFDGLTLRIEHPFAFADFTSISAYRYFTT
                     FNREEQDGTNRLYLYFDDVNIEQNKSFSQELTLAGKTSVADWVAGASYYYDDAHQTSQ
                     LNLFTNSIDTLLNNTGLVPGGVYGPISAAAAQFGIPVTLLGDPWQENMINHGYSRAGA
                     LFGDVIWHLAERWNLTTGVRFTHDEKDFSWYNPLRTATQLDAGLAELTSLGFPLPPFP
                     FQQNLEFTTPISTAAPLRLKNSWNDTSPRFVLDYKPLADVMLYASVARGYEAGGYNAL
                     EPGAKYDPETVRNYELGVKSELFEHRLLLNSSVYYYLYSNLQSLNLVSNGNGSLPAYE
                     VTVSDQHAKGVDFEARWQATTALHLNFLAAYIDDTYKDYIAPDGTNLAGQATGEPLWS
                     LAAGFDYLWHDVASGDLDLTLQDAYRGAGRCNSDSAAQGSCLTIPTFRIGLAQNRTDA
                     RLGWSSHGRVPVTVALYVNNLFDKQYATGVQTISATTLGTPFTNITAPRFWGVEIGAH
                     F"
BASE COUNT          647 a         1059 c         1073 g          659 t
ORIGIN      
        1 gtcaatgcgc gcgcgcggca tccacagata gtcaatcgtt gtgcagattt gtcctgcatt
       61 cagaagtttc acgaacagga tccgctccgc cgcggtgcgc agtggaaaac cctcgcacaa
      121 cagtgcgggc gatttgccgc cgagctccaa cgtaaccgga acgagattgc cggcggccgc
      181 gctcatcaca ctgcggccgg tctgcgtcga tccagtgaac agcaagtggt cgagctgcag
      241 ccgcgagaat tcgatcccaa ccccacccgt ctcatcgaac acggcgagct tatcggcggg
      301 aaaatatcgc ggcaggcgtt cgatgagaag tgccgcgagg tgccgcgagt tttccgacag
      361 cttgatcatg gcgcggttgc ccgcggcgaa aatcgacgtc agcggagaga acgccaaatt
      421 aatcggaaaa ttccacggca cgataattcc cacgatcccc agcggctgcg gccgtagacg
      481 gttgcgcgcc cccgggaaca acaaaaggtc gacgctgcgc cgttgcgggc gcatccaggc
      541 acgcagattt ttcaacgcat gcttggttcc atcgagcacg ggaacgagtt cggtaaacag
      601 agtctcgtgg cgcgaacgat tgccatagtc ggcgttcacc gcctcgatca gcgggtcgcg
      661 attctcgagg aggaagcgcc gtagagtgcg caaatccgcc acccgctgcc gatacgtcgg
      721 caccggatcg cgcgtgtacg cctggcgctg cttgtcgagt gccgcctgca gctgcggacg
      781 cacattcggt gttgccgcgg atacggcacc atcctgaggt cttgcgctca ctgcagtccc
      841 ctcccgtttc tcagaaatgc gcaccgatct caacgcccca aaaacgcggc gcggtgatat
      901 tggtgaacgg cgttccaagc gtcgtcgcgc tgatggtctg cacgccggtc gcatattgct
      961 tgtcaaaaag attgttcacg tacagcgcca ccgtgaccgg cacgcgtccg tggctcgacc
     1021 acccgaggcg tgcatcggtg cgattttgcg ccaaaccgat gcgaaacgtc ggaatcgtca
     1081 agcaactccc ctgcgccgcc gaatcgctgt tgcagcgccc tgccccgcgg tacgcatcct
     1141 gtagagtcag atccaggtct ccgctcgcca cgtcgtgcca caggtagtcg aagcccgcgg
     1201 cgagcgacca cagcggctcg cccgtcgcct gccccgcgag gttcgtgccg tccggggcaa
     1261 tgtagtcttt gtaggtgtca tcgatataag cggcgagaaa attcaggtgc aacgccgtgg
     1321 tggcctgcca gcgagcctcg aaatccacgc ccttggcgtg ctgatcgctc acggtcactt
     1381 cgtaggcggg aagcgagccg ttgccgttcg acaccaaatt gagggactgc aggttcgaat
     1441 acaggtaata gtaaacggat gagttcagga gcagccggtg ctcgaaaagc tcgctcttga
     1501 cgcccagctc gtagttgcgg acggtctccg gatcgtactt cgccccgggt tcgagcgcat
     1561 tatagccgcc cgcctcatag ccgcgcgcaa ccgaggcata caacatgacg tcggccagcg
     1621 gcttgtagtc gagcacgaag cgcgggctgg tatcgttcca gctgttcttg aggcgcaacg
     1681 gtgcggccgt cgagatcggc gtagtgaact cgaggttctg ctgaaatggg aacggcggca
     1741 gcggaaaccc caaggaagtg agttcagcca ggcccgcatc gagctgcgtc gccgtgcgca
     1801 acgggttgta ccaactgaaa tccttctcat cgtgcgtgaa gcgtacgccc gtggtcagat
     1861 tccatcgctc ggcgagatgc cagatcacgt cgccaaacag cgcgccggcg cgtgagtagc
     1921 cgtggttgat catattctcc tgccacggat ccccgagcaa ggtgaccggg ataccgaact
     1981 gcgccgcggc tgcgctgatc ggcccgtaca cgccgccggg cacgagcccc gtgttattga
     2041 gcagcgtgtc gatactgttc gtgaacagat tcagctggct cgtttgatga gcatcgtcat
     2101 agtaatagct cgcgcccgcg acccaatccg cgacgctggt cttgccggcc agggtcaact
     2161 cctgcgagaa actcttgttc tgctcgatgt tgacgtcgtc gaaatacaga tacagccggt
     2221 tggtgccgtc ctgctcctcg cgattgaagg ttgtgaagta acgataagcg ctgatcgatg
     2281 tgaaatcggc gaatgcaaat gggtgttcga tgcgcagcgt caggccgtcg aacttgcgtg
     2341 tctcgcgccc atcgatcaca tcgttcaaga ccggtgcatt aaacggactg atccaggtgt
     2401 tgggagtcga gggaaatggc ggcaagcccg gcgacggcgg cagcgtcgcg atgccgatgg
     2461 cgggtcgcgc cggttgatcg aggtcctcgt gttcccagat cagccgaacg cgggtgtcgc
     2521 cgggcgctcg ccataggagc tgggctcgcg ttccccaatc tccgttgcga tcgtagcggg
     2581 cgccggtggc gctgtcctga agccagccgt cgctgtggtt gtcgacgaag gtcacgcgtg
     2641 cagccagatc cgggccgagc ggcgcattca gcatggcttc gtagtacttc tcgccaaaat
     2701 tgccgaagcg cgctttcgcg tctcccgccc acgtatcggt cggttcattg gtgatcaccg
     2761 aaatggcgcc cgcggcactg ttgcgtccga agagtgtgcc ctgcgggccc ttcagtacct
     2821 cgacgcgttg cacatcgttg aatagcagca gcgcgccgcc ggtcttgcct gtatagacac
     2881 cgtcttcata cagaccaatc ggcgaatcgg tgccgatgcc gaagtccgaa acgctgatgc
     2941 cgcgcagcgt gaagccaggc tgcgtcggct gttcgccgct cacgtcgagc ccgggaacat
     3001 agccgttcat ggtggctaaa tcggtggcgg cgagcttctc gatctgctgg ctggtcacga
     3061 cctgcagcgc gatcggcacg tcctgcgatg cttcggtgcg ccgctgtgcc gtgatggtga
     3121 tggtggcgag ttcgccctcg gctgcgggca tcgacgcttg ttcgctcgtg gcgtctgcgg
     3181 cgacggcgcc gccgctaccc gcgcacagcg cgagcacgga caggtaaata gaacggcggc
     3241 gcgtaaacat tctgagctct ccggtgtcgt tagttttata tgccgcactg tccaggcatg
     3301 gcggcaatcc aatcgcgaat caaggcgagg ccctcgcgat gcacggtgct gcgcccctgc
     3361 tccggcatca ttacgccggg ctcgcgcgag ctcatgcgaa acggcaggat cgactcctcc
     3421 gggtgtcccg gcacaatg
//
LOCUS       DASWZT010000102         5303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_82960, whole genome shotgun sequence.
ACCESSION   DASWZT010000102 DASWZT010000000
VERSION     DASWZT010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82960"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1521)
                     /gene="der"
                     /locus_tag="VGE92_02660"
     CDS             complement(139..1521)
                     /gene="der"
                     /locus_tag="VGE92_02660"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020752350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEY1312745.1"
                     /translation="MLPVLALIGRPNVGKSTLFNAMTGTRDALVADLPGLTRDRQYGF
                     ARRTDIPCIVVDTGGLVENADDIESLMVSQTERAISEADRLIVLVDGRAGVTPQDQFV
                     AQLARRSGKPVYLAVNKTEGFDHDIAVADFHQLGLGEPQPIAAAHDQGVISLLETALA
                     GLTADPGDGPETTGIKVAVIGRPNVGKSTLINRLLGDNRMVAFDQPGTTRDAVLVPFE
                     RDGERYTLIDTAGLRRRSRVEEVVEKFSVIKTLQAIETANVVVVVMDAHDSVAEQDAS
                     LLGTVIDQGRALVLAVNKWDNIPTEQRDLIRQQITQRLQFADFAPLHFISARHGSGVG
                     ELFAAVREVYAAATRDMSTPELTRVLEAAIESHQPPLVSGRRIKLRYAHQGGRNPPVI
                     VIHGNQTDHVPDAYRRYLVNVYRKAFDLMGTPVRIAFRGDENPYAGKRNPLTPRQIRK
                     RKRLIQRNKR"
     gene            complement(1526..2872)
                     /gene="bamB"
                     /locus_tag="VGE92_02665"
     CDS             complement(1526..2872)
                     /gene="bamB"
                     /locus_tag="VGE92_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HEY1312746.1"
                     /translation="MRSLLPLGLAALLIVAGCSKDKDVEPPATLVKFPAKLPVKKLWS
                     EGVGGGKKQIVLRLGLGPAIDNGMVFAASHKGEVLAASLDTGHHVWEKKLKIPISAGP
                     GAGAGMVVLGSSKGTVVALDGATGRELWRSKINSELLSAPAVSEKIVVVRSVDGRLHG
                     LDAHSGKELWSVEQQVPRLSLRGTATPIIAKEVAISGFDNGKVMAVSLNNGDTVWDTA
                     LASPHGRTELDRLVDIDSEVHAVGDNVFATGFQGRTAMLALDSGQIWWAHDMSSYRGL
                     AVDADNLYVTQSDGIVVALRQRDGSELWRNQKLKLRRLSTPVLTSSAVVVADFQGYVH
                     WLDKITGELVARERIAKERITNSPAAFDDTIVVLTDGGKLGAYRASPVAAAAANAAVP
                     AAEPAPAPAATSPAAPAAAEPSPAPPAAAPATPAESAPAPPVESAPAAPPITPPTP"
     gene            complement(2872..3555)
                     /locus_tag="VGE92_02670"
     CDS             complement(2872..3555)
                     /locus_tag="VGE92_02670"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1312747.1"
                     /translation="MTEEYLTDDEQLEAVKRAATEYAPWLIGGVVAGAIAFFGIRYYQ
                     TYTNDRALQAAARFSAMTLAMQANDESKSRQIAAGLIKDYASSPYADQAQLTLARIAV
                     EDGHLDQAVAPLTQVMNNSKDSELRQVARLRLARVLIDQGRPDDALKTLPDPIAASFA
                     APAHAVRGDAYLAKKDNAHALSEYQAALGTDSAGSNSALLELKIQDLGAAPAAPPAIS
                     ADFPNKAKP"
     gene            complement(3566..4846)
                     /gene="hisS"
                     /locus_tag="VGE92_02675"
     CDS             complement(3566..4846)
                     /gene="hisS"
                     /locus_tag="VGE92_02675"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEY1312748.1"
                     /translation="MSKTIEPLRGVHDVLPAHIPAWQHLERVTREVFAGYGYEEFRVP
                     VIEQTQLFKRSIGDFTDIVEKEMFSFVDQGEDHITLRPEATAGIVRAVISNGMLREGR
                     LRVWCMGPMFRRERPQAGRFRQFHQIDAEAFGFPGPDVDAEMILLSARLLRRLGLTRI
                     KLLLNSLGTPESRAAYREQLSAYFSAHAAALDEDSKRRLQGNPLRILDSKNPDMQRLV
                     AAAPRLLDSLDAESRTHFESLCAHLRSAGIEYHIEPHLVRGLDYYTRTVFEWTTDALG
                     SQSTVCAGGRYDGLVAQLGGVSTPGIGWAMGQERIVMLLEKQALSKQRVPPHVYMVLA
                     GEHEIEGFKLAEQLRDAWPALALQINLGGGSFKTQFKRADKSGAQFAIVLGDDEIARG
                     VAAVKALRQESAQEEWPIAQFSERLGVLLGLKGG"
     gene            complement(4860..>5303)
                     /locus_tag="VGE92_02680"
     CDS             complement(4860..>5303)
                     /locus_tag="VGE92_02680"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HEY1312749.1"
                     /translation="PAAALAAAPQPPAAGSASAAAPSGAAAPPAGAEAADAAAGTGRA
                     RLRLSFSADSWVDIHDASGRRAYAGNGRANSVKTIAGDGPMRVYLGFASGVQLEINNR
                     AVAIGPQFVAGDVARFEAGADGVLRRDTRSLSTPTAQTRNARPIG"
BASE COUNT          927 a         1749 c         1637 g          990 t
ORIGIN      
        1 catcaaccgt attcgatcgg ccactcgggc tcgcgccgag cacgcctttc acgtggtgaa
       61 gaatctctgg ggtttcacca aggtgcgcta cctacaccca atggggcctg cactcgccac
      121 cgcctgtgca gagtttccct agcgcttatt acgctgaatc agccgcttgc gcttgcgtat
      181 ctggcgcggc gtcagcggat tgcgcttgcc ggcataggga ttctcatcgc cacggaacgc
      241 gatgcgcacc ggcgtgccca tcaaatcgaa agccttgcga tacacgttca cgagatagcg
      301 gcggtaggcg tccggcacgt ggtcggtttg attgccgtga atgacgatca cgggcggatt
      361 gcggccgcct tgatgcgcgt agcgtagctt gatgcgtcgt ccgctgacca acggcggctg
      421 atgactttcg atggctgcct cgagtacccg cgtcaattcc ggggtgctca tgtcacgcgt
      481 ggcggcggca tacacctccc gcaccgccgc gaacagttcg ccgacgccgc tgccatgacg
      541 tgccgaaata aaatgcaacg gtgcgaaatc ggcgaattgc aggcgctgcg tgatctgctg
      601 gcggatcaag tcgcgttgtt ccgtcggaat gttgtcccat ttgttgacgg ccagcacgag
      661 ggcccgcccc tgatcgatga cggtccccag caggcttgca tcctgttcgg caacgctgtc
      721 gtgggcgtcc atcactacca cgacgacgtt ggcggtctcg atcgcctgca gagtcttgat
      781 gacgctgaat ttttcaacca cctcttcgac gcgcgagcgg cgcctcagtc cggccgtgtc
      841 aatcaacgta tagcgctcgc catcacgctc gaacgggacc agcacggcat cgcgggtcgt
      901 gcctggctga tcgaaggcga ccatgcggtt gtcgccgagc aggcggttga tcaaggtact
      961 cttgccgacg ttcgggcggc cgatgacggc cactttgatg cccgtcgttt caggaccgtc
     1021 cccgggatca gccgtcaatc cggcgagtgc cgtctcgagc agcgagatca ccccctgatc
     1081 gtgcgcggcc gcgataggct gcggctcgcc caacccaagt tggtgaaaat cggctacggc
     1141 aatgtcgtgg tcgaaaccct cggtcttgtt gaccgccaag tacaccggct tgccgctgcg
     1201 gcgcgccagc tgcgccacga attggtcttg aggcgttacg ccggcgcgcc catccacgag
     1261 cacgatgagc cgatccgcct cgctgatcgc gcgctcggtt tgcgaaacca tcaggctctc
     1321 gatgtcgtcg gcgttttcga ccaaaccgcc ggtatccacg acaatgcacg gaatgtcggt
     1381 gcgccgcgcg aacccatact ggcggtcgcg agtaagcccc ggcagatcgg caaccaacgc
     1441 atcacgcgtg ccggtcatgg cgttgaacaa ggtcgatttg cccacgttgg gccgaccgat
     1501 caatgcgagt accggcagca cgacgctacg gcgtcggcgg agttatcggc ggtgctgcgg
     1561 gcgcggactc gacaggcggc gcgggcgcag attcggccgg cgttgcgggc gcagccgcag
     1621 gcggcgccgg ggagggctcg gctgctgcgg gcgctgccgg tgaggtcgct gccggtgcgg
     1681 gcgcaggctc ggcagcaggc acagcagcgt ttgccgcggc agctgccacg ggcgaggccc
     1741 gatacgcgcc caatttgccg ccgtcggtca gcacgacgat ggtatcgtcg aacgccgccg
     1801 gcgagttggt aatgcgttcc ttggcgatgc gttctcgggc gacgagctcc ccggtgattt
     1861 tgtcgagcca gtgcacgtag ccctgaaagt cggccaccac gaccgcgctg ctggtcaaca
     1921 ccggggtgct caaacgccgt aatttcaatt tctgattgcg ccacaactcc gacccgtcgc
     1981 gctgacgaag tgcaaccacg atgccgtcgg attgcgtcac ataaaggttg tccgcatcga
     2041 ccgccaagcc tcgatagctc gacatgtcgt gcgcccacca gatttgtccg gaatcgagcg
     2101 caagcatcgc ggtgcgtcct tggaatcccg tggcgaacac gttatcgccc acggcgtgca
     2161 cctcagaatc gatgtccacc agccgatcga gctccgtgcg gccgtgcggc gaagcgagtg
     2221 ccgtatccca aaccgtatcg ccgttgttga gcgagaccgc cattaccttg ccgttgtcga
     2281 agccgctgat cgccacttct ttggcgatga tgggcgtcgc tgtgccgcgc agactgagcc
     2341 ttggcacctg ttgctcgacc gaccacagct ccttgccgct gtgtgcgtcc aagccatgca
     2401 gccggccgtc cacggaacgc accacgacga ttttttcact gaccgccgga gccgacagca
     2461 gctcggaatt gattttcgag cgccacagct cgcggcccgt ggccccgtcc aaggcgacga
     2521 cagtgccctt gctgctgccc aaaacgacca tgcccgcacc agcgcccgga ccggctgaaa
     2581 taggaatctt aagcttcttc tcccagacgt gatggcccgt gtcgagcgac gcggcgagca
     2641 cctcgccctt gtgcgaggcg gcgaagacca tgccgttgtc gatcgcaggc cccaacccta
     2701 agcgcagcac aatctgcttc ttgccgccgc cgacgccttc gctccaaagt ttctttaccg
     2761 gcagcttcgc cggaaatttc accaatgtcg ccggcggttc gacgtctttg tctttgctgc
     2821 agccggccac gatcagcagc gccgccagac ctaagggaag cagggaacgc atcagggttt
     2881 tgccttgttt ggaaagtcgg cggaaatagc gggcggagcc gctggggcgg caccgagatc
     2941 ctgaatcttg agttcgagca gcgcggagtt gctgccggcc gagtcggtcc cgagagcagc
     3001 ctggtactcg ctgagcgcgt gagcattgtc cttcttcgca agataggcgt cgccgcgcac
     3061 cgcgtgggca ggcgccgcga aagacgccgc gatcgggtca ggcaaggttt tcaatgcatc
     3121 gtccggtcga ccttgatcga tcaacacccg cgccaagcgc aggcgcgcca cctgccgcag
     3181 ctccgagtcc ttcgagttgt tcatcacttg cgtcaaaggc gcaaccgctt ggtcgaggtg
     3241 gccgtcctcc acggcgatcc gcgccagggt gagctgcgcc tgatcggcat agggtgagct
     3301 tgcgtagtcc ttgatgaggc ccgccgcgat ttgccgcgat ttgctctcat cgttcgcctg
     3361 catggcaagc gtcatggcgc tgaatcgcgc cgccgcttgc agggcacgat cgttggtgta
     3421 ggtctggtaa tagcggatac cgaaaaatgc tattgcaccg gcgacaaccc caccgatcaa
     3481 ccacggggcg tactccgtcg ccgcacgctt gacagcctcc aattgttcgt cgtcggtcaa
     3541 atattcttct gtcatggctt ctccgctacc cgcccttaag acccaacaag acccccaagc
     3601 gttcactgaa ttgcgcgatc ggccattctt cttgcgccga ttcctggcgc aaggccttta
     3661 cagcggccac gccccgggcg atttcgtcat cccccaatac gatcgcaaac tgcgcgccgc
     3721 tcttatcggc gcgcttgaat tgcgtcttaa aactcccgcc gccgagattg atttgcagag
     3781 cgagagccgg ccaggcgtcg cgcaattgct ccgctagctt gaagccttcg atctcgtgct
     3841 caccggcgag caccatgtac acatgcggcg gcacgcgttg tttgctcaac gcctgttttt
     3901 cgaggagcat cacgatccgc tcctggccca tcgcccagcc gatacccggc gtactgacgc
     3961 cgcccagctg cgccaccaac ccatcgtagc gccccccggc acacaccgtg ctctgcgagc
     4021 cgagcgcgtc cgtcgtccac tcgaaaaccg ttcgcgtgta gtaatcgaga ccgcggacca
     4081 ggtgcggttc gatatgatac tcgatgccgg cactgcgcag gtgcgcgcac agcgactcga
     4141 aatgcgtgcg cgactctgca tccagggaat caagcaagcg cggcgctgcc gcgacgagtc
     4201 gctgcatgtc cggattcttg ctgtcgagta ttcgcagcgg attgccctgc agccggcgtt
     4261 tactgtcctc gtcgagcgcc gccgcgtgcg cactgaagta agcgctgagc tgctcgcgat
     4321 aggcggcgcg cgactcggga gttcccaagg aattgagcag cagcttgatg cgcgtcaggc
     4381 ccaggcggcg cagcaagcgc gcggacaaca gaatcatttc ggcatcgaca tccggtccgg
     4441 gaaatccgaa tgcttctgcg tcgatttgat ggaattggcg aaaacgtccc gcctgcggtc
     4501 gttcgcgccg gaacatcggt cccatgcacc acacgcgcag ccggccctcg cgcagcattc
     4561 cattcgagat gaccgcgcgc acgataccgg cagtggcttc agggcgcagc gtgatgtggt
     4621 cctcgccctg atcgacgaag ctgaacatct ctttttcgac gatatccgtg aagtcgccga
     4681 tcgagcgctt gaacaattgc gtctgctcga tcacggggac gcgaaactcc tcatatccgt
     4741 aacccgcgaa aacctctcga gtgacacgct ccaagtgctg ccaggcaggg atgtgcgcgg
     4801 gcaacacatc gtgcacgcca cgcagaggct cgatagtctt gctcaacagc gcgctccggt
     4861 cagccgattg gtcgcgcgtt gcgcgtctgc gcggtgggcg tcgacaggct gcgggtatcg
     4921 cgccgcaaga cgccgtcggc gcccgcctcg aagcgcgcca cgtcgccggc gacgaattgc
     4981 ggtccgatgg cgaccgcacg gttgttgatc tcgagttgca cgccgctcgc aaagcccaga
     5041 tacacgcgca tgggcccgtc accggcgatc gttttgacgc tattggcccg accgttgccg
     5101 gcgtacgcgc gccggcccga cgcatcgtga atgtccaccc aggaatcggc ggaaaagctc
     5161 agacgcagcc gggcgcggcc ggttcctgcg gccgcatcgg cggcctccgc gccggccggt
     5221 ggcgcggcag cacccgaggg cgccgcggcg cttgcgctcc cggctgcggg gggctgcggc
     5281 gctgcggcca gcgccgcagc cgg
//
LOCUS       DASWZT010000103         4087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_15224, whole genome shotgun sequence.
ACCESSION   DASWZT010000103 DASWZT010000000
VERSION     DASWZT010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15224"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..1096
                     /locus_tag="VGE92_02685"
     CDS             227..1096
                     /locus_tag="VGE92_02685"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY1312750.1"
                     /translation="MAGWEDGDWRNEDLCKFGESNLLNKMGLEFYDLSHPWGLGQPCW
                     PYFEDVKIERLHGMSRSGVLTQKITTVMHSGTHIDAPAHVVPGTPFMDEVPLPYFFGS
                     GVVVSIPKKKWEVITAKDLENARPRIRPGDIVIVNTGWHKYYGDNQHYYGYSPGFYKE
                     AGEWFVERQVKMCGSDTQALDHPLGTAIGPHGPGAPHGLLPRLNEEYERETGHKVIED
                     FPFWEPCHNAILSAGICGFENIGGDIDKVTGKRLTFAAFPWRWKKGDGCIVRLVAMLD
                     PSGNFRIETGRDA"
     gene            1110..1460
                     /locus_tag="VGE92_02690"
     CDS             1110..1460
                     /locus_tag="VGE92_02690"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY1312751.1"
                     /translation="MQITRFADARRYDAPKHFDMRSLRLQGFDLSAAKFAWVGVSHFL
                     PQGGAQMDAGPLEKIYVVLEGAVTIELADGTTHVLHPLDSCHIPGGEARSIRNDGNAV
                     ASMLVIMPHPEPRP"
     gene            1457..2257
                     /locus_tag="VGE92_02695"
     CDS             1457..2257
                     /locus_tag="VGE92_02695"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY1312752.1"
                     /translation="MTLPKIDHAGLFDVRGKSAIIVGATGAFGKVACSTLGSAGAKLT
                     VAAGNAAELAVLARELAAAGIDAQQIARRSNNEADAASIVQAAVQAHGGIDILVVASG
                     MNDVSPIVEMAPERFARVMQANVEGAWLIARAAGQQMIAQARGGKVVFTSSARGKLGH
                     PAGYSAYCASKSAVDGMTKALGCEWGKHNITVNAIAPTVFRSPLTAWMFEDNENANKV
                     RAGFLARVPIGRLGEPEDLAGPLLFLCSRASNFHTGHIIYADGGYTAG"
     gene            2263..3180
                     /locus_tag="VGE92_02700"
     CDS             2263..3180
                     /locus_tag="VGE92_02700"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY1312753.1"
                     /translation="MAPSPSIAILGGGLMGAGIAQVFAAAGHSVTVFEPLADVRRTIR
                     ARIAADLRLIQREEALADAVRITDDLAESVSRADYVTEAVPEKLELKQSIFADLVRWA
                     PPTAILASNTSVIPIHRIAAGLETAERIVGTHWWNPPGLIPLVEVIQSARTSLATVTA
                     TMALLTAIGKAPAHVTKDVPGFVANRLQHALWREAIAMVAEGVCDAATLDACVKNSFG
                     LRLPVLGPLENADLVGLDLTLDIHKTIIPELDRLDRPNPYLEEQVAAGRLGFKSGVGF
                     RAWSADEMQALREKLAAHLLAARQKREPP"
     gene            3180..>4087
                     /locus_tag="VGE92_02705"
     CDS             3180..>4087
                     /locus_tag="VGE92_02705"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1312754.1"
                     /translation="MLAATARTTRRYEIRAVGILSLGFGLVGIDRFLITTMFPVIAKD
                     LHLDYGDIGAITGALAIAWGLSALFMGNLSDHIGRRVVLTVSLIVFSLLIGASGLATG
                     LMGLIAVRVVMGLADGAYTPVSIAGTMAASAPQRRGLNIGIQQMMMPLFGLGLAPLFV
                     ASVLHVLDWRYIFLIFVLPGLALSFAMWKLIPARAIPASRNVHGGRLADWKTALGYAN
                     VRIAAAVMLCWLTCLITTSAFMPNYLVDYLHLPLPQMAKIMSAIGLGATTGTLLLPWA
                     SDRFGRRSVMAFSSIGVLLSLVLLVNT"
BASE COUNT          740 a         1266 c         1274 g          807 t
ORIGIN      
        1 ctgcacggtc gagcgcggca ggtcgacgcg ggccgcgatg tcacccaagc tcaggccatt
       61 cggttcttgt tcgagcgagc gcagcaccgc cgcagcgcgc gcgattactt gaattccgcc
      121 ccgttctacg gcactcatcc accctacctc gcttgactaa gtgacttcat tgagcatacc
      181 gtatgccaat acagtttaac cgcatcatgg ttcataggag tataacatgg ctggctggga
      241 agacggtgac tggcgcaatg aggatctgtg caagttcggt gaatcgaacc tgctcaacaa
      301 aatgggtctc gagttctacg acctgagcca tccatgggga ctcggtcagc cgtgctggcc
      361 ctacttcgag gacgtaaaga tcgagcggct gcatggcatg tcgcgctccg gtgtcttgac
      421 gcaaaaaatc accactgtca tgcattccgg aacgcatatc gatgcaccgg cgcacgtcgt
      481 gccgggcacg ccgttcatgg acgaggtgcc gttgccctac ttcttcggca gcggcgtggt
      541 ggtatcgatc ccaaagaaaa aatgggaggt catcaccgcc aaggatctcg agaatgcgcg
      601 tccgagaatc cgtccgggcg acattgtcat cgtcaacacc ggctggcata aatattacgg
      661 cgacaaccag cattactacg gctactcgcc cggcttttac aaggaagccg gtgagtggtt
      721 cgtcgagcgt caggtaaaga tgtgcggttc cgacacccaa gcgctcgacc acccgctggg
      781 gacggcaatc ggaccgcatg ggcccggcgc gccgcatggc ttgctgccgc ggctcaacga
      841 agaatatgag cgcgagaccg gccacaaggt gatcgaggat ttccccttct gggaaccctg
      901 ccacaatgcg attttgtcgg ccggaatttg tggtttcgag aacatcggcg gcgacatcga
      961 caaggtgacc ggcaaacgcc tgacgttcgc ggcgtttccg tggcgctgga aaaagggcga
     1021 cgggtgtatc gtacgcctgg tcgcaatgct cgatccgagc ggtaacttta ggatagaaac
     1081 cggccgcgac gcataatcgg aggcggcaca tgcaaattac ccgtttcgcc gacgcgcgcc
     1141 gttatgacgc gccgaaacac ttcgacatgc gctcgctgcg gctgcagggc ttcgatctca
     1201 gcgcagcgaa atttgcctgg gtgggagtgt cgcacttcct gccgcagggc ggcgcacaga
     1261 tggacgcggg cccgctcgag aagatctacg tcgttctcga gggcgcagtc acgattgaac
     1321 ttgccgacgg aacgacccac gtgctgcacc ctctcgacag ctgccacatt cccggcggag
     1381 aagcgcgttc gatacgcaat gacggcaacg cggtggccag catgctcgtc atcatgccgc
     1441 atcccgagcc ccgaccatga cattacccaa gatcgatcac gccgggctat tcgatgtgcg
     1501 cggcaagtcg gccatcatcg tgggcgccac cggcgccttc ggcaaggtgg cgtgctcaac
     1561 gctgggatcc gccggagcca aactcaccgt cgccgccggc aatgcggcgg aattggcggt
     1621 actcgctcga gagcttgccg cggcgggcat cgacgcgcag cagatcgcgc gccgctccaa
     1681 taacgaggca gacgccgcga gcatcgtgca ggcggcggtg caagcccacg gcggcatcga
     1741 tattctcgtg gtcgcgtccg gcatgaatga cgtatcgccc attgtcgaga tggcgccgga
     1801 gcgctttgca cgcgtaatgc aggcaaacgt cgaaggtgcg tggctcatcg cacgtgcggc
     1861 gggccagcaa atgattgcgc aagcgcgcgg cggtaaagtg gtgttcacct cgtcggcccg
     1921 cggcaaactc ggccatccgg ccggctattc ggcttactgc gcgtccaaat cggcggtcga
     1981 cggcatgacc aaggcgctcg gttgcgaatg gggcaagcac aacatcaccg tcaacgcgat
     2041 tgcgccgacg gtgttccgtt cgccgttgac cgcgtggatg ttcgaggaca acgagaacgc
     2101 gaacaaggtg cgcgcgggct tcctcgcccg cgtgccgatc ggacgactcg gagagccgga
     2161 ggatttggcg ggaccgctgt tgttcttgtg ttcgcgcgcg tcgaactttc ataccgggca
     2221 tatcatttac gccgacggcg gctacaccgc aggatagccc gcgtggcgcc atcgccttcc
     2281 attgcgatct tgggtggcgg gctgatgggc gccggcattg cgcaagtgtt cgccgcggcc
     2341 ggacactcgg tcacggtctt cgagccgctg gccgacgtgc gccgcacgat ccgcgcacga
     2401 atcgcggcgg atttacgctt gattcagcgc gaagaggcgc tggcggatgc cgtgcgtatc
     2461 accgatgact tggccgagtc ggtgtcgcgc gcggactatg tgacggaggc cgttcccgag
     2521 aaactcgaac tcaagcagtc gatttttgcg gatttggtgc gctgggctcc gcccacggcg
     2581 atcctcgcgt cgaatacctc ggtcattcca attcatcgga tcgcagccgg gctcgaaacg
     2641 gcggagcgca tcgtcggcac acactggtgg aatccgcccg gcctcatccc cctggtcgaa
     2701 gtcatccaat cggcacggac ctcacttgcg accgtaaccg cgaccatggc gttgctgact
     2761 gcgatcggca aggctccggc ccacgtgacc aaggacgtac caggattcgt ggcgaaccgc
     2821 ctgcagcatg ccctgtggcg cgaagcgatc gcgatggttg ccgagggcgt ctgcgatgcc
     2881 gcgacccttg atgcctgtgt gaagaacagt ttcggcttgc gtttgcccgt cttgggtccg
     2941 ctcgagaacg cagatctcgt cggcttggac ctcaccttgg acattcacaa aaccatcatt
     3001 ccggaactcg atcggctgga tcgccccaat ccgtacttgg aggagcaagt ggccgccggg
     3061 cggctggggt tcaagtccgg cgtgggattt cgcgcatggt ctgccgatga gatgcaggcg
     3121 ctgcgcgaga agcttgcggc gcacctgttg gcagcgcggc agaaacgcga gccgccgtga
     3181 tgctcgccgc taccgctcgc acgactcggc gctatgagat acgcgcggtc ggcattcttt
     3241 cattgggctt cggcctggtc ggcatcgacc gatttctcat cacgaccatg ttccccgtga
     3301 ttgcgaagga cctgcactta gactatggcg acatcggcgc cattaccggg gccctggcca
     3361 tcgcctgggg tttgtcggcg ttattcatgg gtaatttatc cgatcacatc gggcgccgcg
     3421 tggtgctcac ggtatcgctc atcgtgtttt cgcttctcat cggagcctca ggtctcgcca
     3481 ccggattgat gggtttgatt gcagtgcggg tcgtcatggg gcttgccgac ggtgcctaca
     3541 caccggtcag catcgccggt accatggccg cctccgcgcc gcaacgccgc ggcctgaaca
     3601 tcggcatcca acagatgatg atgccgctgt tcggtttggg cttggcgccg ctgttcgtcg
     3661 cgagcgttct gcacgtcctc gactggcgct acattttcct gatcttcgta ctgccgggct
     3721 tggcgctcag tttcgcgatg tggaagctca ttccggcgcg cgcaatcccc gcaagtcgga
     3781 acgttcacgg cggccgcctc gccgactgga agacggccct cggctacgcc aacgtacgca
     3841 tcgctgcggc cgtgatgttg tgctggctca cgtgcctgat cacgaccagc gccttcatgc
     3901 ctaactatct ggtcgattac ctgcatctgc cgctgccgca aatggccaag atcatgtcgg
     3961 ccatcggtct cggggcgacc accggcacat tgctcctgcc ttgggcctcc gatcggttcg
     4021 gccgcaggtc cgtcatggcg ttctcgagca tcggcgtatt gctgtccttg gtgctgttgg
     4081 tcaacac
//
LOCUS       DASWZT010000104         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_68447, whole genome shotgun sequence.
ACCESSION   DASWZT010000104 DASWZT010000000
VERSION     DASWZT010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68447"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="VGE92_02710"
     CDS             complement(<1..928)
                     /locus_tag="VGE92_02710"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY1312755.1"
                     /translation="MKYVTSKCAGLGAWALSLGLVLSLGLVLSLDACAVGPNFKPPAA
                     PQSAGFTPAGQIAAQTTAASLPGGEAQRFVDGMDIPGEWWTLFKSRPLNDLIERALQN
                     SPNLQAAQAALRQANETAAAERGSYYPSVSGSLESQRQKASGAAFGIPGFPASYFYNL
                     DTASVNVSYTLDAFGGIRRQVEALNAQAEYQRFALEASYLTLTANIVTAAVNEASLRA
                     QIAATEQISGAQQTQLGIVQRRLTAGGASRVDLLQQQAALQSTLVSLPPLRSQLAQQR
                     NQLAALVGSLPADYSGGEFTLDSLDLPQELPVS"
     gene            1184..1657
                     /locus_tag="VGE92_02715"
     CDS             1184..1657
                     /locus_tag="VGE92_02715"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY1312756.1"
                     /translation="MPDLPLTEVLLCRVMLHLGREMAAMLEQQIRPFGLAEAEFRVLT
                     TLFSQPDGVANPSELCARASQSPANMSRISDALVSRDLITRVLSAHDRRRMVLRITEQ
                     GEELVRRLLPIMFAPLRTLFAEFPEPAQRQLLAQLKRLYANFAQLQKHELVERDP"
     gene            1654..>2220
                     /locus_tag="VGE92_02720"
     CDS             1654..>2220
                     /locus_tag="VGE92_02720"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY1312757.1"
                     /translation="MNRWQCVGAAIALLGVGCAGLPPKPKPVSLSVAVPLDGLEAAGG
                     DWPAPEWWKRYQDPTLDQLIELAVGSSPTLATAHARFDSARQSVRIAGAAAGARVDAS
                     ADFDRQRLSDNGLFPPQLLGFSWYNQADLGLQASYTFDWWGKQRDAVEAAVDQAHAAQ
                     ADRSAAALTLASSIADTYFGWQADEARLS"
BASE COUNT          401 a          667 c          721 g          431 t
ORIGIN      
        1 cgctcaccgg tagctcctgc ggcaggtcga gcgagtcgag cgtgaattcg ccgccgctat
       61 aatccgcggg caaagagccg accaaggccg ctagttgatt gcgttgttgt gcaagctggc
      121 tacgcaaagg cggaaggctg accaaggtgc tttgcagcgc ggcctgctgc tgtagaagat
      181 cgacgcgcga ggcaccgccg gcggtgaggc gcctctggac gatgccgagt tgagtttgct
      241 gcgcacctga aatctgttcg gtggccgcga tctgagcgcg cagtgaagcc tcgttgacgg
      301 ccgccgtgac gatgttggcg gtgagcgtga ggtagcttgc ctcgagcgca aagcgctgat
      361 attccgcttg agcattcagc gcttccactt gacgacggat gccgccaaac gcgtcgagcg
      421 tgtacgacac gttcaccgag gcggtatcga ggttgtagaa ataggacgcc gggaatccgg
      481 gaatgccgaa cgcggcgccc gaggcttttt ggcgttggct ttcgagtgaa cccgagacac
      541 tcgggtaata actgccgcgc tccgcggccg cagtttcatt tgcttgtcgc agcgccgcct
      601 gcgcggcttg cagattcgga ctgttttgca gcgcgcgctc gatcaaatcg ttcaaggggc
      661 gcgatttgaa caaggtccac cattcgcccg gaatgtccat gccgtcgacg aagcgctgcg
      721 cttcgccgcc cggcaaagac gccgccgtcg tctgcgcagc gatctgaccg gcaggtgtga
      781 agcctgcgct ctgcggcgcc gcggggggtt tgaaattcgg gccgaccgcg caggcgtcga
      841 ggctcagcac gaggccgagg ctcagcacga ggccaaggct caatgcccag gcgccgaggc
      901 cggcacattt cgatgtgaca tatttcacgg cttagacacc agtcagattg gttggtcgat
      961 ggcccgacgc ggcgtcccaa cccgtgcgca tcgagccttc ggcgcgcatg acgctcattc
     1021 aggcctaaaa aatgacatgc ggtgttttcc attgcaagaa ctatttacat gctggatatt
     1081 catttggtaa aatatattcc aacgaatggt tagtatgcaa atgaataatc gcgatgctca
     1141 attcgataag ctggaggaga acctccgagc cctgagcacg cgcatgccgg atctaccgct
     1201 cacggaagtc ttgctgtgcc gcgtgatgct gcacctcggg cgcgaaatgg ctgccatgct
     1261 cgagcagcag attcgaccct tcggtctcgc cgaggctgaa tttcgcgtgc tgaccacctt
     1321 gttttctcag cccgacgggg tggcgaatcc gagcgagctg tgcgcccggg cgtcgcaaag
     1381 cccggccaat atgtcgcgca tcagcgatgc actcgtgagc cgcgacctca tcacccgcgt
     1441 tttgagcgca cacgatcggc gccgcatggt gttgcgcatc acggagcagg gcgaggagtt
     1501 ggtgcgccgt ttgttgccga tcatgttcgc gccgctgcgc acgctgttcg cggaatttcc
     1561 ggagcccgca caacgccagc tcctggcgca gctgaagcgc ttgtatgcga attttgcgca
     1621 gctgcagaag catgaattgg tcgagcggga cccgtgaacc gctggcaatg cgtcggcgcc
     1681 gcgattgcac tgctcggcgt cggttgcgcc ggactgccgc cgaagccaaa acccgtttct
     1741 ctgtcggtag cggtgccgct cgatggtctg gaggcggcgg gcggcgattg gccggcgcca
     1801 gaatggtgga agcgttatca ggatccgacg ctggaccaat tgatcgaact cgcggtcggt
     1861 tcctcgccga ccttggcgac ggctcacgcg cgcttcgatt cggcgcgcca gtccgtgcgc
     1921 atcgccggcg cggcagccgg agcgcgcgtc gatgccagcg ccgatttcga ccggcagcga
     1981 ctcagcgata atggcctgtt tccgccgcag ctgctcggct tcagctggta taaccaggcc
     2041 gatttaggac tgcaagccag ctacaccttc gattggtggg gcaagcagcg cgatgcggtc
     2101 gaggcggccg tggaccaggc gcacgccgca caggcggacc gcagcgccgc cgcattgacg
     2161 ctcgctagtt cgatcgccga cacctacttt ggctggcagg cggatgaagc tcgtttgtcc
//
LOCUS       DASWZT010000105        10688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_82994, whole genome shotgun sequence.
ACCESSION   DASWZT010000105 DASWZT010000000
VERSION     DASWZT010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10688
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82994"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VGE92_02725"
     CDS             complement(<1..200)
                     /locus_tag="VGE92_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HEY1312758.1"
                     /translation="MTSATAILTVHTAAVAPSITTQPANQSVTAGQTATFSVVAGGTA
                     PLSYQWQKGGAAIAGATAPSYTT"
     gene            355..528
                     /locus_tag="VGE92_02730"
     CDS             355..528
                     /locus_tag="VGE92_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312759.1"
                     /translation="MEKLAICPASTVAPAGWSVMLGRTAIALLVAPVLLGASALMGAC
                     VLLLPEQPASSEV"
     gene            676..1545
                     /gene="hpnK"
                     /locus_tag="VGE92_02735"
     CDS             676..1545
                     /gene="hpnK"
                     /locus_tag="VGE92_02735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis-associated protein HpnK"
                     /protein_id="HEY1312760.1"
                     /translation="MNNYLIVTADDFGLHESVNEAVERAGRAGTLNAASLMIAAPAAK
                     DAIRRARLLPNLRIGLHVVLADGWSTLEGHVIPQIADQSGRMSGRMLGKSVRFFVNSA
                     AREQLDAEIRAQFAAFARTGLRLDHVNVHKHFHVHPTVLSILLQVARDFGTPAIRVPY
                     EPFWFAARSGGLTAGIAQGFLAPWIAFMKHRLRSAGVFHNDRIFGMASSGHMSEAALL
                     AILARLPGGVSEIYLHPATESGSAIAPSMGSYRHAVELAALLSPRVRAALGAITFRGG
                     YLDVLRAVGRSLA"
     gene            complement(1905..3575)
                     /locus_tag="VGE92_02740"
     CDS             complement(1905..3575)
                     /locus_tag="VGE92_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEY1312761.1"
                     /translation="MADFYSISPVAAPIKGGAEEVGNKAWNLMRLAQAGMPVPPAFVL
                     PTAWCHRAKSTLSPQLDKVLADGIAQLESATRLGFGAAYRPLLVSVRSGAAVSMPGMM
                     ETLLDVGLNAVTVDALIRLTGNPRLAWDSYRRLVQGYAEIVAHLPTAPFDQLVAAELQ
                     RAETDNEDELDHRQLRALTQAMLGCYSALTGSSFPVDPYEQLRTATVAIFSSWDAPKA
                     VSYRRLNNLSDAAGTAVTVQTMVYGNAGGESGAGVAFTRNPATGTREMYFDFQFNAQG
                     EDVVSGRQHLRDNERLQRVLPAVWLRLNDICRKLEVLFGDVQDFEFTIQAGELFLLQT
                     RRAKRTDWAALTIAVDMVEEGLLTPVDAIARLKGIVLASIVRTSFALPIPQNLAVAQV
                     AGMGVASGAIALDSEAAKRLAAPGRPVIFVRRQTVTTDIEGMALADGILTGSGGRTSH
                     AAVVARQLGKVCLVACPTLEIDLEHRQCHIGGQLLREGDLLSLDGNTGAVYPGRIVAL
                     SEHPAAALATIARWNKATSGEFDVRAHTANVTVPVLPQGPNALTPEFP"
     gene            complement(3568..5376)
                     /locus_tag="VGE92_02745"
     CDS             complement(3568..5376)
                     /locus_tag="VGE92_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEY1312762.1"
                     /translation="MKYRFSLIQMALSHAEHPDQPAATLRQERIACGIDDAQLDGVVA
                     AARMVGTACHITGAAQIVVRIADDMRIMAAPILDTKSNGLAARLDGLLRALPAAANDL
                     LEPSVISAITQISQTKADSLHQLVMELHKRLNAMQADLAQESLDGAAAYNLAEEDRPL
                     VLAFMKGVNRTAKLKFAHPGLSTTATRAGDQLVIQNDLGTTDAHVIVIHVKKLTVTVT
                     YTDVHSERLAFFQEMLKGQSIIWDQERQAVLAATEPFYLATGRVEVTAGAARATFLEF
                     LGSRLVFLIDWNRARKQLRGFLRRADRLVLLHWAAEAEVGHRGFLELGGARPINQAVE
                     ATAGSSMHWGDRLCEVLGDAESLEFLRFVLRASTEGLLSGSSRTLIHDRIRVTLATHF
                     SNEERQLLRLAADHAGLIFEIAGLVQSGVQGGSGTAENRARRAGRYEHDADQIVAETR
                     DAVRRRPDYSVFLRVLEASDDAADELEDAAFLLNLDTLEGKPLEALQNLANCLVDASQ
                     EWIKALGHAAQIGHPTGAAETEDFLNAIDRISVLEHEADGAQRNVAATAVQHAKNFRQ
                     LHLFAAIGGKLEAAADAIKHASLILREHVLEDVIDG"
     gene            complement(5672..6838)
                     /locus_tag="VGE92_02750"
     CDS             complement(5672..6838)
                     /locus_tag="VGE92_02750"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1312763.1"
                     /translation="MNFIKQFLGILGVSLAGVTQRLGSALTIVIGVACAVGALVAMLA
                     MGVGATQQIVGEPRPDRVLVTGSGSHGGMMSNIPQDEAATVHDLPGIAKSADGAPVVV
                     FQSFVPLEGRRRASGVRIYFPLIGVTPNVTQYSPEMHFTEGRMFKSGLQELIASNPCV
                     RQFTDFELGAQRRIHGASWTVVGHFDQGESQQCVVFADVGIINSTFLRNSYSEVRVRL
                     QSAADYGAFRDAVTSNPTLHLDVEHESDFNERESKQINGILNFVSYFVGVIMAIGATL
                     GAVNSLYAMVDSRRRELATLRAMGFGSGAIVASILCESVVLALPGALLGCLLAWALFN
                     GLAVSPFGFNFQLKVTAYLAVLGVIWALAMGLIGGLLPALRAARVPVTTALRAT"
     gene            complement(6839..7981)
                     /locus_tag="VGE92_02755"
     CDS             complement(6839..7981)
                     /locus_tag="VGE92_02755"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY1312764.1"
                     /translation="MKFLPLIWSGIWRKPGRTILIFLQVSVAFALFGVLQGLKSGVEQ
                     SISETRADLLLVHSSLSLGDSLPLGLLEQIKSVAGVKVVIPVGLLGSVYQKPDQHATI
                     VAIRPDPGWMSAFTYTIAPEAEAAFKKSRTAALVNAGIVTKYGFRVGDRIPLNTPLAQ
                     ASGSTVWTFDMVGTFTDSDFGAADNNILVNFDYFDQARAVGKGTVAHYNVTIADPKLA
                     ASVEDEIDRQFANSANETRTDSLRQLAQVQMQSIGDLNFLIRAIVSAVFVALLFATGT
                     MMMQSIRERTPELAVLKTLGFGDRTLVMLILAEAVAVCIAAAATGLVLATLVFPFAAK
                     IVRGISMPFVVVELGLAYAVVVALVSAAVPAVRAARLQVVSALAER"
     gene            8093..>10688
                     /locus_tag="VGE92_02760"
     CDS             8093..>10688
                     /locus_tag="VGE92_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY1312765.1"
                     /translation="MNHNQNDADAALPWSDESCFPLDWNGPVGRPFDCFPEEAKARPI
                     AELLAAVVRRHPQRIALLGKDTSLTYAALWREVGGWAEFIAGASAPGALVGIRAGVSP
                     AFPIALLGCLAAGRPFLAIDPDYPPELIERVLDDARPALLLEPTQARLASPGWQPVSL
                     APDAPACVLYTSGSTGEPKGIVNSQRNLLQRVCQSINAAHIGSDDRFLTLAPLSSIVG
                     LRDVLTALLAGACICLIDQKAGARDILRIIDEQRISILFSFPALLRSVVESRQARAGD
                     HLRLVRIGGDTTLWSDIALLRDWMPPGALVQLVYAATEAPMMQWFVGHSAPEDDARIP
                     IGYPLPGNALAVVDEHGAATRPGQAGELLVRSAYVALGTWRRGGCVADPGQSSAGDRG
                     MRIFRTGDLVRRRPDGLFDRIGRKDRQVKIRGARVELDGVEASIRRHAAVRDVGVVVR
                     SDEDGARLVAYVEPHDRAADDFLGELEAMMRRTVPPHLRPSRYYATPRIPRLPNSKLD
                     GLALRAMDLAKSLNEETRGARRSATWIERDPVEIVVAHIWRDVLGLQLGGAEDDFFDL
                     GGDSLRAITMTVELEKTLGRELPMNLINQSPTFAAFCAALKDNVRPVYSPLVVLKPGR
                     GPTPVFFIHGVGGGVMELFGIGRKMTWPGAVIGIQARGLDGRDPPHDSVQAMTEEYHA
                     AVKARQPQGPYLLCGYSFGGLVAFELARRMSEQGDEVAFVGLLATLPPGHHFLRLWTW
                     GSYLCRQIAQGFAAGERPAEMREPRANDAAPSALRKVKASALSASIRYRPGKYRGALT
                     LFEPGSRDLRLPSSARLWRRHAAALKHYSLQGRHDDMLHGSNADAAAALLTRCLEESL
                     VMAPSDARI"
BASE COUNT         2048 a         3327 c         3282 g         2031 t
ORIGIN      
        1 gtcgtatagc ttggcgcggt ggctcccgcg atggcggcgc cgcctttctg ccattgatag
       61 ctcagcgggg ctgtaccgcc ggcgacgacc gagaaggttg ccgtctgtcc ggcagtgacg
      121 ctctgattcg caggctgagt ggtgatgctc ggtgcgaccg ccgcggtatg caccgtgaga
      181 attgctgtag cgctcgtcac ggagcctaac gagttgctga cgagcaccgt aaatgcggag
      241 ccgttgtcag cggctgtggt cgcacccgtc acgtagccgc tcgaagtcgc gccggcgata
      301 tttacaccgt ttctgcgcca ttggtaactc agtggcgaag tgccggacgc agtgatggaa
      361 aagctggcga tttgcccggc gagcacggtg gcaccggccg gctggagcgt gatgctgggc
      421 cgcaccgcga tcgcgctgct ggttgcgccc gtactgctgg gcgcgtccgc gctgatgggc
      481 gcctgcgtgc tgctgcttcc agagcagccg gctagcagcg aggtgtagac tagcgccgca
      541 aggaaccgcg ttgacggcgc gtccttgcgt ttgactttca ttgcgattac tttgtggcgc
      601 aaagcaagtg gagtcaagag tctctcaatt gcgacttgtc gccacctgac gaatatcgtg
      661 gcgtactccc atcgaatgaa taactacttg attgtcacgg cggatgactt tggcctgcac
      721 gagtcggtga acgaagcggt cgaacgcgcc ggccgcgccg gaaccctcaa tgcggcgagc
      781 ctgatgatcg cggcaccagc ggcgaaagac gccatccgcc gggcgcgcct actgccgaat
      841 ctgcgcattg gcctgcacgt ggttctcgcc gacgggtggt ccacgctcga gggacacgta
      901 attccgcaga ttgcggatca atccggacgc atgagcggcc gcatgcttgg caagagcgtt
      961 cggttctttg tcaattctgc ggcacgcgag cagctcgatg cggagatccg cgcccaattt
     1021 gcggcattcg cgcgcaccgg attgcggctc gaccatgtga acgtccacaa gcattttcac
     1081 gttcacccca cggtcttgag cattctgctg caggtcgcac gcgacttcgg tacgccggcc
     1141 attcgggtgc cgtacgagcc cttctggttt gccgcgcgca gcggcggctt gaccgccgga
     1201 atcgctcagg ggttcttggc gccgtggatc gcgttcatga agcaccgcct gcgctccgca
     1261 ggggtgtttc acaacgatcg gattttcggc atggccagca gcggccacat gagcgaagcg
     1321 gcgttgctcg ccattctggc gcggctaccc gggggagtct ctgagatcta tttgcatccg
     1381 gcgaccgaat cgggcagtgc gattgcgcca tcgatgggtt cgtatcggca tgcggtggaa
     1441 ctcgcggcgc tattgagtcc aagagtgcgc gcggcactcg gcgcgatcac ttttcgcggc
     1501 gggtacctgg atgtgctgcg tgcggtggga cgatctctgg cctgagtgac ggcccggcat
     1561 gcaaaaatat atctttgtca tgatggcagc gtaacctaag gatgccggtt agcgatgaca
     1621 cgcggctgga gctggcggac gcgataattg cgtcacaaac agatcgatca atgcccgtag
     1681 aagccgtctg ggctgaacgt tcttatcttc aaaagtatcg agcaccatgg acgaattagt
     1741 agactctcaa atcctttcgc aagagcaatg ggccattgaa ttgctggctc gcgaaaccca
     1801 taccgcaatt ggaaaagtgc aggaggtctt ccttgttgag tataagaaac ttgcggcgca
     1861 tgcgcacatc acgtcgtacc tccccctgct ggcgtgcaac agcgttaggg gaattctgga
     1921 gttagcgcat tcgggccttg aggtaaaacc ggcaccgtga cattagccgt gtgggcccgc
     1981 acatcgaatt cgccagaagt ggctttattc cagcgggcaa tcgtcgccaa ggcagccgcg
     2041 ggatgttcgc tgagtgccac aatacgaccg ggatacacgg cccccgtatt gccatcgagc
     2101 gacaataaat caccttcgcg cagtaactgt ccaccaatgt ggcattgccg atgttccaaa
     2161 tcgatttcaa gcgtcggaca cgcaacgagg cacaccttcc ccagctggcg tgcgacgact
     2221 gccgcatgcg atgtgcgtcc gccggagccg gtcaatatcc cgtccgccag ggccatgccc
     2281 tcaatgtccg tcgttacggt ttggcgacgc acaaatatca cgggtcgtcc aggcgctgcc
     2341 aagcgtttgg ccgcttccga gtcgagtgca atcgcaccgc tggcaactcc cataccggcg
     2401 acttgggcaa cggccaagtt ctgagggatc ggtaaggcaa agctcgttct cactatgctg
     2461 gcgagtacaa ttcccttgag gcgtgcaatg gcgtcgaccg gcgtcagcag gccctcctcg
     2521 accatgtcta cggcaatggt cagcgcagcc cagtcggtac gtttggcgcg ccgggtctgg
     2581 agtagaaata gttcaccggc ttgaatcgtg aactcgaaat cttggacgtc accgaacagc
     2641 acttctaatt tgcgacagat gtcattcaga cgcaaccaga ccgccggtaa cacccgctgc
     2701 agtcgctcat tgtcgcgcag gtgctggcgc ccgctcacga catcctcgcc ctgggcattg
     2761 aattgaaaat cgaaatacat ctcgcgcgtt cctgtggcgg gattgcgcgt aaaagcgacg
     2821 cctgctcccg attcaccgcc cgcgttgcca tacaccatgg tttgtactgt tactgcagta
     2881 ccggcggcat cgctgagatt attcaagcgg cggtaactta ccgccttggg cgcgtcccag
     2941 gaactgaaaa ttgccaccgt cgccgtgcgg agctgttcat acggatcgac cggaaaggaa
     3001 ctgccagtca acgcagagta acagccgagc atcgcctgcg ttagcgcgcg caactgtcga
     3061 tggtcaagct catcttcgtt atctgtctcg gcgcgctgca actcagctgc cacgagctga
     3121 tcgaacggcg cagtgggtaa atgggccacg atctctgcgt aaccttggac gagccggcga
     3181 taactgtccc atgcgagtcg gggattgcct gtcaagcgga tgagggcgtc gaccgtaacg
     3241 gcattcaacc ctacgtccaa gagcgtctcc atcatgccgg gcatggacac tgcagcaccg
     3301 gagcgcacag agacgagcaa tggacgatag gcggcgccga aacccaggcg agtcgccgat
     3361 tccagctggg cgatgccgtc ggccagaacc ttatccaatt gtggggagag tgtcgattta
     3421 gctcggtggc accaagccgt cggtagcacg aacgccggcg gcaccggcat gcccgcctgt
     3481 gccaggcgca tcaagttcca cgccttgttg ccgacttcct ctgcgccgcc tttgataggc
     3541 gccgctaccg gtgatatgga atagaagtca gccatcgatc acatcttcga gtacgtgctc
     3601 acgtaagatc aagctcgcat gcttgatcgc gtcggctgcg gcttcgagct tgccgccaat
     3661 cgcggcaaat agatgcagct gacgaaaatt tttcgcgtgc tgaaccgcgg tcgcggcgac
     3721 gttacgctga gcaccgtctg cctcatgttc gagtacacta atccggtcaa tcgcattcag
     3781 aaagtcctcc gtctcggccg cgccggtggg gtggccgatc tgagcggcat gtccgagcgc
     3841 cttgatccat tcctgtgaag cgtcaaccag acagttggcc agattctgca atgcctccaa
     3901 gggcttcccc tcaagagtgt caagattgag aaggaaggca gcgtcctcca actcgtccgc
     3961 cgcgtcgtct gaggcctcca acacgcgtaa aaacaccgaa tagtcggggc ggcgacgcac
     4021 ggcatcacgg gtttctgcaa caatttggtc cgcatcatgc tcgtaccgac ctgcccttcg
     4081 agcacgattc tctgcagttc ccgacccgcc ttgcacgccg ctttggacca agccggcaat
     4141 ttcgaatatc agccccgcgt ggtcggcggc gagacgcagg agttggcgtt cctcgttgct
     4201 gaaatgggtg gcaagcgtga ctcggatgcg gtcatggatc aacgtacggg aactccccga
     4261 tagcagccct tccgtggacg cccgaagaac gaaacgtaga aactccagac tctcggcatc
     4321 acccagcacc tcgcagagtc gatcgcccca atgcatcgat gatcccgcgg tcgcctcaac
     4381 cgcctgatta atggggcgcg cgccgcctaa ctccaaaaaa ccacggtgcc caacctctgc
     4441 ttctgcggcc caatgcaata gcaccaagcg gtccgcgcgc cgcaggaagc cacgcaattg
     4501 tttgcgcgct cgattccagt cgatcaagaa caccagccgt gatccgagaa attcgagaaa
     4561 cgttgcgcgt gccgcccccg cggtcacttc aacgcggccg gtcgcaagat aaaagggctc
     4621 agtcgcggct aacaccgcct ggcgctcttg atcccagatg atagattgac ccttcaacat
     4681 ctcctgaaag aatgccagcc gttcgctgtg gacatcggta tacgtgacag tgacggtgag
     4741 tttcttcaca tggatgacga ttacgtgggc atcggttgtt ccaagatcgt tttgaatcac
     4801 aagctgatcg ccggcgcgag tggccgttgt tgagagtccg ggatgcgcga acttaagttt
     4861 cgcagtccgg ttgactccct tcatgaaggc caacaccaga gggcggtctt cttcagctaa
     4921 gttgtaagcc gcggcgccgt ctaggctctc ctgcgccaaa tccgcttgca ttgcgttgag
     4981 ccgcttgtgc aattccatga cgagttgatg caggctgtcg gcttttgtct ggctaatttg
     5041 cgtgattgcg gaaatgacgg agggctccag caaatcattg gccgcagcag gcagcgcgcg
     5101 caacaatccg tcgagccgtg cagccaatcc gttcgatttg gtgtcaagta tgggagcggc
     5161 cataatgcgc atgtcgtcgg cgatgcgcac gacgatctgc gctgcgccag tgatgtggca
     5221 cgccgtgccg accatgcgcg cagcagcgac gacgccgtca agctgcgcat catcgatgcc
     5281 acaagctatc cgttcttggc gcagggtggc cgccggttgg tccggatgct cggcatggga
     5341 aagggccatt tgtatcaagg agaagcgata tttcacccga tcattcgcgg cgagggcggc
     5401 gttcagacgg gtcgcagggt gcagttcatt ctcgccgatt gcggccagaa tctgggtttt
     5461 catgtggctc tcggtgttct cgacgcggat tgcatacagg cgggcacatg cgttgtaaca
     5521 cgcgccgcac aaatcctacg tgagtcgata aaagggatct gtacgttagg ccacggacat
     5581 caaattgcaa caatatgcag aggggctggt gcctcatcga tccaacccct ttgcacaaca
     5641 tcgggccctg cggtggggct cgcggcgctg ttcacgtcgc gcgcagcgcc gtcgtcaccg
     5701 gtacgcgcgc cgcacgcagc gcgggaagca gtccgccgat gaggcccatc gcgagcgccc
     5761 agatcacgcc gagcactgcg aggtaggcgg tcactttgag ttgaaaattg aagccgaagg
     5821 gactcaccgc caagccgttg aaaagcgccc acgccaaaag acatccgagc agtgcgccgg
     5881 gtagcgccag aaccaccgat tcacacagaa tcgaggcgac gatcgccccg gaaccgaagc
     5941 ccatcgcccg caacgtcgcg agctcgcggc ggcgcgagtc caccatcgcg tacagcgaat
     6001 tgaccgcgcc caacgtggcg ccgatcgcca tgatcacgcc gacgaagtag ctgacgaaat
     6061 tcaagatacc gttgatttgc ttcgactccc gctcattgaa gtcgctttca tgttcgacgt
     6121 cgaggtgcag cgtcggattg gaggtgacgg catcgcggaa ggcgccgtaa tccgcagctg
     6181 attgcagcct cacccgcacc tcggagtagc tgttgcgcag gaaggtcgaa ttgatgatgc
     6241 cgacgtcggc gaaaaccacg cattgttggg attcgccctg atcgaaatgg ccgaccaccg
     6301 tccagctcgc cccatgaata cgccgctgcg cacccagctc gaagtcggtg aattggcgca
     6361 cgcaggggtt gctcgcaatg agttcctgca gaccgctctt gaacatgcgc ccttcggtga
     6421 agtgcatttc cggggaatat tgcgtcacgt tcggcgtcac cccgatgagc ggaaaataga
     6481 ttcgcacgcc gcttgcgcgc cgccgcccct cgagcggcac gaacgattgg aacaccacga
     6541 ccggcgcgcc gtccgcactc ttcgcgatgc cgggcaaatc atgcacggtc gccgcctcgt
     6601 cttgcgggat attgctcatc attccaccgt gcgagccgct gccggtcacg agaacccgat
     6661 cgggccgcgg ttcgccgacg atctgctgcg tcgccccgac gcccatcgcc agcatggcga
     6721 ccaacgcgcc gaccgcgcac gcaaccccaa tgacgatggt cagtgccgaa cccaatcgct
     6781 gcgtgacgcc cgcgagactg acgcccagga tgcccagaaa ttgtttgatg aaattcatct
     6841 agcgctcggc caatgcactc acgacctgca gcctcgccgc ccgcaccgcc ggcacggcgg
     6901 cgctgaccaa ggccacgaca acggcgtacg cgaggccgag ttcgacgacg acgaagggca
     6961 tcgaaatgcc gcgcacgatt ttcgcggcga acggaaacac cagcgtggcc agaaccaatc
     7021 ccgttgccgc cgcggcaatg cacactgcca cggcctcggc gagtatcagc atgaccaggg
     7081 tgcgatcgcc gaagccgagg gtcttcagca ccgcgagttc cggcgtgcgt tcgcggatcg
     7141 attgcatcat catggtgccc gtggcgaaca gcaacgcgac gaacacggcg ctgacgatcg
     7201 cacgtatcaa aaaattaaga tcgccgatcg actgcatttg tacctgtgcc agctgccgca
     7261 acgagtcggt gcgtgtctcg ttggcggaat tggcgaactg ccggtcgatc tcatcctcca
     7321 cactggcggc gagcttggga tccgcaatcg tcacgttgta atgcgccacg gtgcctttgc
     7381 cgacggcgcg tgcttggtcg aagtaatcga aattgaccag gatgttgttg tccgcggcgc
     7441 cgaaatcgct gtcagtgaag gttcccacca tgtcgaacgt ccagacggtc gaaccgctcg
     7501 cctgcgcgag cggcgtattc aacgggatgc gatcgccgac tctaaaaccg tacttagtga
     7561 cgattcccgc gttgacgagg gcagcggtgc gcgatttttt gaaggcggct tcggcctcgg
     7621 gcgcaatcgt gtatgtaaac gcggacatcc agcccggatc cgggcgaatc gccacaatgg
     7681 tcgcgtgctg atccggcttt tgatagacac tcccaagcag tccgaccggg atcaccactt
     7741 ttacgcccgc aaccgacttg atctgctcga gcaagcccag cggcaaggag tcgccgagac
     7801 tcaagctgga atgcacgagc agaagatcgg cgcgcgtctc gctgatcgac tgctcgacgc
     7861 cggatttcaa cccttgcagc acaccgaata gcgcaaaggc gacgctgacc tgcaggaaaa
     7921 tcaagatcgt ccgcccgggt ttgcgccaga taccggacca gatgagcggc aaaaatttca
     7981 agcagccccg cgaaccatcg tcattcccag ccagccagtc ggatgcttgc agtataggac
     8041 gcaagtgagc gagcgcgcgt gcaactttgc ggcggccgct gtgttctagg acgtgaacca
     8101 caatcagaac gatgcggatg cggcgctccc gtggagcgat gagagctgct ttcccttgga
     8161 ttggaacggc cccgtcggcc ggccgttcga ttgcttcccg gaagaagcca aggcacggcc
     8221 catcgccgag ctgttggcgg cggtggtgcg ccgccatccg caacgcatcg cgctcttagg
     8281 aaaggacacc tccctcacct acgccgcgct ctggcgcgag gtgggcggct gggcggaatt
     8341 cattgccggg gccagcgcgc cgggcgcact ggtcggcatt cgggccggcg tgtccccggc
     8401 atttccgatt gcgctgttgg gctgcctggc ggcaggcagg cccttcctcg caatcgatcc
     8461 tgactatccg ccggagctca tcgagcgggt gttggatgac gctcgtccgg cgttgctgct
     8521 cgagccgacc caagcgcgcc tcgcctcgcc cggctggcaa ccggtcagcc tcgcccccga
     8581 tgcgcccgcc tgcgtgctct atacctcggg cagtaccggc gagcccaagg gcatcgtcaa
     8641 ttcgcagcgc aatcttttgc agcgcgtctg tcagtcgatc aacgcggccc atatcggcag
     8701 cgacgatcga ttcctgaccc tcgcacccct ctcgagcatc gtcggcttac gggatgtatt
     8761 gaccgcgctc ctcgcgggag cctgcatctg cctcatcgac cagaaggccg gcgcgcgcga
     8821 catcttgcgc atcatcgatg agcagcgcat ctcgatttta ttcagcttcc cggcgctgct
     8881 gcgctccgtc gtcgagagcc gtcaagcccg cgccggcgat cacttgcgcc tggtgcgaat
     8941 cggcggcgac acgacgctgt ggagcgacat tgcgctgctg cgcgattgga tgccgcccgg
     9001 cgcgttggtt caattggtgt acgccgccac cgaggcgccg atgatgcaat ggttcgtcgg
     9061 gcacagtgcg cccgaggacg atgcgagaat tccgatcggc tatccgctgc cgggcaacgc
     9121 cttggcggtg gtcgatgagc acggcgctgc gacacgaccc ggccaggcgg gtgaactgct
     9181 ggttcgcagc gcctacgtcg cgctcgggac ttggcggcga ggaggctgcg tggcggatcc
     9241 cgggcaaagc agcgccggcg atcgcgggat gcggatcttc agaaccggcg atctggtgcg
     9301 acggcggccc gacggcttgt tcgatcgcat cgggcgcaag gaccggcagg tcaagatccg
     9361 cggtgcgcgg gtggagctcg acggcgtcga agcctcgatt cgccgtcacg ccgccgtgcg
     9421 cgacgtgggc gtcgtggtcc gctcggacga agacggtgcg cgactggtgg cctacgtcga
     9481 accgcacgac cgagcggccg atgacttcct cggcgagttg gaggccatga tgcgccgcac
     9541 cgtcccgccg cacctgcggc cctcgcgcta ctatgcgacg ccgcgcattc cgcgtctgcc
     9601 caattcaaaa ctcgacggac tcgcgctgcg cgccatggat ctggcgaaga gtctcaacga
     9661 ggaaacccgt ggcgcgcggc gcagcgcaac gtggatcgaa agagatcccg tcgaaatcgt
     9721 cgtcgcgcat atttggcgcg acgtcctcgg attgcaactc ggcggagccg aggatgactt
     9781 cttcgatctc ggcggcgatt cgctgcgcgc catcaccatg accgtggagc tcgagaaaac
     9841 gttgggccgc gagttgccca tgaatctcat caatcaatcg cccacctttg ccgcgttctg
     9901 cgcagcgctt aaggacaatg tgcggcccgt ttattcccct ctcgtcgtct tgaaacccgg
     9961 ccgcgggccg acgccggtgt tcttcattca cggcgtgggg ggcggggtga tggaactgtt
    10021 cggcatcggc cgcaagatga cctggccggg cgcggtcatc ggcattcagg cgcggggcct
    10081 cgatggccgc gatccgcctc atgacagcgt gcaggcgatg accgaggaat atcacgccgc
    10141 cgtgaaagcg cgacagccgc aagggccgta tctgttgtgc ggctattcct tcggcggcct
    10201 cgtggccttc gagttggcgc ggcgcatgag cgaacaaggc gacgaagtcg cctttgtcgg
    10261 cttgctcgcc accttgccgc cgggccatca tttcctgcga ctgtggactt ggggctctta
    10321 tctttgcagg caaatcgccc agggcttcgc cgcgggggaa cggccggccg agatgcggga
    10381 gccgcgcgca aatgacgccg cgccgtcggc attgcgcaag gtcaaagcga gcgccttgtc
    10441 ggcctcgatc cgctaccggc cgggaaaata ccgcggcgca ttgacgctgt tcgagccggg
    10501 cagtcgcgac ttgcgcctgc cctcctcggc gcggctctgg cgccgccacg ccgctgcgct
    10561 caaacactac agcctgcaag gccgccacga cgacatgttg cacggatcca acgccgacgc
    10621 ggcggctgcg ctattgaccc gctgtctcga ggagtcgctc gtgatggcgc cgagcgatgc
    10681 tcgtatcg
//
LOCUS       DASWZT010000106         2317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_78189, whole genome shotgun sequence.
ACCESSION   DASWZT010000106 DASWZT010000000
VERSION     DASWZT010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78189"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..1505)
                     /locus_tag="VGE92_02765"
     CDS             complement(63..1505)
                     /locus_tag="VGE92_02765"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY1312766.1"
                     /translation="MTKPASRREFLKMAAALSATRFAPPLALNLAALGESAAAGATDY
                     KALVCVFMIGGNDNFNTLVPYDAANYALYTQQRPAIATALSALTPTLLTPATALPGGE
                     QFALAPALSPLLPLFNGGQMALMLNIGTLIEPIASAAAYASAPATSLPPQLFSHLDQR
                     AFYQAEGPTGNTSGWGGRIADLFASGNGNSLFTCISPAGDSLFLTGLNTGTYSISPTG
                     GAIPIQSIVSPLFGSSACQNLMRTLTTAQRTDASGNLANLFEATYADVVSRSLTGQQI
                     LGAALPAATVTTFPTTTKTNLSAQLSMVARVIAARNASGIGATRQVFMVGAGNFDTHD
                     AVVTLQPPLLSDVAVSMAAFYAEMNALGLQNNVTSFTVSDFGRTLNSDGDGSDHGWGS
                     VHFVIGGAVNGNNFYGTAPVLPPNLTTPGPLDVGRGRFVPTMAVDQFGATLGSWFGVS
                     NADLLTIFPNLSNFDASTRNLGFMQAPTSG"
     gene            complement(1517..>2317)
                     /locus_tag="VGE92_02770"
     CDS             complement(1517..>2317)
                     /locus_tag="VGE92_02770"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HEY1312767.1"
                     /translation="GQTIAAGLTGEQTLQAAIGILMAHPNMAPFISRQFIQRLVTSNP
                     STDYVSRVATVFESTQGDMKSVITAILTDTEATSAALLSNPMAGKLREPVVRFIQWAR
                     TFPLNFSPATATQWAAIGDLSNPSSRLAESPLQSPNVFFFFQPGYIPPHTALQANGIT
                     APEFAITNESSVAGYMNYIKNLIANGIGGVAADYSALQPLVNQGGNSAALLAELNVLI
                     AAGQLSAATLSLMQGALDTIDVITMGTNDRLFAALTLVMCAPEYLVQK"
BASE COUNT          438 a          673 c          805 g          401 t
ORIGIN      
        1 acaccaagcc tgtgacgagg caccgcagag gttcccccgc gaatcgacgc cgtcgcgccg
       61 gtctacccgc tggtgggcgc ttgcatgaac cctagattgc gggtgctggc atcgaaattg
      121 gaaaggttcg gaaagatcgt caacaagtcg gcattgctca caccgaacca cgagcccaag
      181 gtcgcgccga attgatccac cgccatggtc ggaacgaagc ggccccggcc gacgtcaagc
      241 ggacctgggg tggtcaagtt gggcggcagc acgggggccg tgccatagaa attattgcca
      301 ttgaccgccc cgccgatgac gaagtgaacg ctgccccagc cgtgatcgga gccgtcgcca
      361 tcggaattga gggtgcgtcc gaaatccgag acggtgaaac tcgtgacgtt gttctgcaag
      421 ccgagtgcgt tcatctccgc gtagaacgcc gccatcgaga cggcgacgtc cgataacagc
      481 ggcggctgca gggtgacgac ggcatcgtgg gtgtcgaaat taccggcgcc gaccatgaac
      541 acctggcgcg tcgcaccgat gccggaggcg ttgcgcgcgg cgatgacgcg cgccaccatc
      601 gataattgcg ccgagagatt cgttttggtg gtcgtgggaa acgtcgtgac ggtcgccgcc
      661 ggcagcgcgg cgcccaagat ttgctgcccg gtcaaggagc ggctcacgac atcggcatag
      721 gtggcttcga acagattcgc gagattgccg ctggcatcgg tgcgctgcgc cgtggtgaga
      781 gttctcatca gattctggca ggccgaggag ccgaataacg gcgagacgat gctctggatc
      841 ggaatcgcgc cgcccgtggg cgagatcgag tacgtgccgg tattgagtcc ggtgaggaac
      901 aaactgtccc cggccggcga gatgcacgtg aacagggaat tgccgttgcc ggaggcaaag
      961 aggtcggcga tcctgccgcc ccaccccgag gtgttgccgg tgggaccctc ggcctgatag
     1021 aacgcccgct gatcgagatg cgagaacagt tggggcggca ggctcgtggc gggcgcgctg
     1081 gcataggccg cggccgaggc aatcggctcg atcagggtcc cgatgttcag catgagcgcc
     1141 atttgtccgc cgttgaaaag cggcagcagc gggctcaacg ccggcgccag ggcgaattgt
     1201 tccccgccgg ggagcgccgt ggcgggagtc aggagcgtcg gcgtcaaggc gctcaaggcc
     1261 gtggcgatgg ccgggcgctg ttgggtgtac agggcatagt tcgccgcatc gtacgggacc
     1321 agggtattga agttgtcgtt gccgccgatc atgaacacgc agacgagcgc cttgtaatcc
     1381 gtggcgcccg ccgcggcgct ctcgccgagc gcagcgagat tcaatgcgag cggcggcgca
     1441 aagcgcgtgg cggacagcgc cgccgccatc ttcaagaatt cgcgtcgcga tgcgggcttg
     1501 gtcacggggg ccgtccttat ttctgaacca aatattcggg agcgcacatc acgagcgtga
     1561 gcgccgcgaa gaggcggtcg ttcgtgccca tggtgatcac atcgatggtg tcgagtgcgc
     1621 cctgcatcaa gctcaaggtc gccgccgaga gttgtccggc ggcgatcagc acgttgagct
     1681 cggcgagcaa ggcggcggaa ttgccgccct ggttcaccaa gggctgcagt gcgctgtagt
     1741 ctgccgccac cccgccgatg ccgttggcga tgaggttctt gatgtaattc atgtagccgg
     1801 cgaccgaact ttcgttggtg atggcgaact ccggggcggt gatgccgttg gcttgcagcg
     1861 cggtatgcgg cgggatgtac cccggttgga agaagaaaaa aacattcggc gactgcagcg
     1921 gactctcggc gagcctgctc gacgggttcg acaggtcgcc gattgcggcc cactgcgtcg
     1981 cggtggcggg cgaaaaattc aatggaaagg tgcgcgccca ttgaatgaat cgcaccacgg
     2041 gttcgcgcag cttgccggcc atggggttcg acaacaacgc agcggaagtg gcttcggtat
     2101 cggtcagaat cgccgtgatg accgatttca tgtcaccttg cgtggactcg aagaccgtgg
     2161 cgacgcgcga gacgtaatcg gtggacggat tgctggtcac gaggcgctga atgaactgcc
     2221 gcgagatgaa cggcgccatg ttcggatgcg ccatcaagat gccgatcgcc gcctgcaggg
     2281 tctgctcgcc ggtcaagcca gccgcgatcg tctgacc
//
LOCUS       DASWZT010000107         2885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_34656, whole genome shotgun sequence.
ACCESSION   DASWZT010000107 DASWZT010000000
VERSION     DASWZT010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34656"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VGE92_02775"
     CDS             complement(<1..342)
                     /locus_tag="VGE92_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312768.1"
                     /translation="MSLRAVLACVCSLAAGAVLAESAYTAADFAQVRKFDAHVHANTD
                     DRRFLDIAAKDGFEILSINVDYPDFPSLDRQAQVAHKLQAADPRRFHFATTFSMHGFG
                     GASWTADTLRHI"
     gene            complement(339..1337)
                     /locus_tag="VGE92_02780"
     CDS             complement(339..1337)
                     /locus_tag="VGE92_02780"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEY1312769.1"
                     /translation="MKIFLGIDGGGTKTDFLLINGSGQVLSSHRGGSAYYLETGLDAL
                     RLMLTAGIQTTLQQAAVPAAGVDFAFIGLPAHGEDSALLPRLDAIAADVLPAARHRCG
                     NDMVCAWAGALAGRDGINIVAGTGSIAYGEFETRGARAGGWGELFSDEGSAHWLAREG
                     LTLFSRMSDGRAPRAALYDLIRERFQLRADLDLCAAIYGPPSLARSEVAALAPLVARA
                     ARAGDAAAYRLFEQAALELAAIVHALRDRLNVPAPLPLPVSYSGGMFHLDGLLTPLLE
                     AALQQGGRRYDFVAPRLPPVAGAALYAAKLAGAALKPAFIAALARATDGGKRMAES"
     gene            complement(1334..2506)
                     /locus_tag="VGE92_02785"
     CDS             complement(1334..2506)
                     /locus_tag="VGE92_02785"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY1312770.1"
                     /translation="MNYLAHPLAELETAGGIWTAREIQQQPAVWADIERLMMHEAAAL
                     RAFLDPLLERQDLRIVLTGAGTSAFVGECLAPALKRRTQRRVDAVATTDLVESPGSWL
                     ERSVPTLLVSFARSGNSPESVAVLTLAEQGVTDCHHLIVTCNGEGDLYRQGQRQRNAH
                     VVLLPEQTNDRGFAMTSSFTGMMLAAALAFDLLPQDRAATLAGFASPVLDAGQLVVSN
                     LVRSGFERVVYLGSLELNGLAREAALKMLELSDGNIVAVAETPLGFRHGPKTIVNGKT
                     LVVMFLSNYPYTRRYEMDLLRELRADGVAARVVALYAEEPGAAQADDALAGGIAVPGS
                     LGASSLALCFPYAMFAQTLAFRQSLALGLRPDTPNARGVVNRVVQGVSIYPWNPSQ"
     gene            complement(2503..>2885)
                     /locus_tag="VGE92_02790"
     CDS             complement(2503..>2885)
                     /locus_tag="VGE92_02790"
                     /inference="COORDINATES: protein motif:HMM:NF019625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HEY1312771.1"
                     /translation="DASLKDTMIEEMRRNPRHWSGYYMDRERQDFDLQFSLSDRIRYY
                     WSSPVVERACTQLLKRLAASRVPLTLISQYLPVQYTAIREGRLTSDPRALVLDAVEQV
                     LRHYDYACRPAGATGSAVTDRGHE"
BASE COUNT          510 a          972 c          953 g          450 t
ORIGIN      
        1 gatgtgccgg agggtgtccg ccgtccagct tgcgccgccg aatccgtgca tcgaaaacgt
       61 ggtggcgaaa tgaaagcgtc gtgggtccgc ggcctgcagt ttgtgcgcca cctgagcctg
      121 tcggtcgagg gatggaaagt cggggtagtc gacgttgatc gatagaattt cgaacccatc
      181 cttcgccgcg atgtcgagaa agcgccgatc gtctgtgttg gcgtggacat gcgcgtcgaa
      241 cttgcggacc tgcgcgaagt ccgccgcggt gtacgcgctt tctgcgagga ccgcgcccgc
      301 cgccagcgag cacacgcacg cgaggacggc gcgcaggctc acgactcggc catgcgcttg
      361 ccgccatcgg tcgcacgcgc gagagcggcg atgaatgcgg gctttagcgc agcgcccgcg
      421 agcttcgcgg cgtacagggc ggcgcccgcc acgggcggca gccgcggcgc cacgaagtcg
      481 tagcgccgtc cgccttgctg gagcgccgct tcgagcaacg gcgtcaacag gccgtccaag
      541 tgaaacatgc cgccggagta ggacaccggc agcggcagcg gcgccggcac gttgagtcga
      601 tcgcgtaggg catgcacgat ggccgccagt tcgagcgccg cctgctcgaa caggcggtac
      661 gccgcggcat cgcccgcgcg ggccgcccgc gccaccagcg gggcgagcgc ggcgacttcg
      721 cttcgagcga ggctcggtgg tccgtagatg gcggcgcaca agtcaaggtc ggcccgcaat
      781 tgaaagcgct cgcggatgag atcgtagaga gccgctctcg gcgcgcgccc atcgctcatc
      841 cgcgagaaca gcgtcagccc ctcgcgcgca agccaatgcg ccgaaccctc atcgctgaac
      901 agctcccccc aaccgcctgc gcgcgcgccg cgtgtctcga attccccata ggcgatcgag
      961 ccggtgccgg cgacgatatt gatgccatcg cggcccgcaa gggcgcccgc ccaggcgcac
     1021 accatgtcgt tgccgcagcg atgacgcgcg gccggcagca catccgcggc aatggcgtcg
     1081 aggcgcggca gcagtgcgct gtcctcgcca tgggcgggca ggccgatgaa ggcaaaatcc
     1141 acgccggcgg ccggcaccgc ggcttgctgt aaagtcgtct ggatgccggc ggtgagcatg
     1201 agccgaagcg catcgaggcc ggtctcgaga taataggctg aaccgccgcg gtgcgatgag
     1261 agcacctgcc ccgacccgtt gatcaacaga aagtctgtct tggtaccacc gccgtcgata
     1321 ccgagaaaga tcttcactga gacggattcc agggatagat cgatacgccc tgcacgacgc
     1381 gattcacgac gccccgtgca ttgggcgtat ccggccgcaa tcccagcgcc aacgattggc
     1441 gaaacgcgag cgtctgcgca aacattgcat acggaaagca cagcgccaga ctggatgcac
     1501 ccagcgaccc cggcacggca atgccccctg ccagtgcgtc atcggcctgc gcagcgcccg
     1561 gctcctcggc gtatagcgcc accacgcgtg cggcgacgcc gtcggcgcgc agttcgcgca
     1621 gcaggtccat ctcatagcgg cgcgtatagg gataattgct gaggaacatg accaccaggg
     1681 tcttgccgtt gacgatggtc ttcggcccgt ggcgaaagcc taggggagtt tcggccacgg
     1741 cgacaatgtt gccgtcgctg agttcgagca tcttgagcgc ggcctcgcgc gccagaccat
     1801 tcaattccag gctgccgagg tacaccaccc gctcgaagcc cgagcgcacc aggttcgaga
     1861 ctacgagctg acccgcatcg agcacagggg aggcaaagcc ggcgagcgtg gccgcgcgat
     1921 cttgcggcaa cagatcgaac gccagcgccg cggcgagcat catgcccgtg aagctcgacg
     1981 tcatcgcgaa gccgcggtcg ttggtctgct ccggcagcag cacgacatgc gcgttgcgct
     2041 gccgctgccc ctgccgatac agatcaccct cgccgttgca ggtgacgatg aggtgatgac
     2101 agtccgtcac gccttgctcg gccagggtga gcaccgcgac gctttccggg ctgttgcccg
     2161 agcgggcgaa cgagaccaac agcgtcggca cgctgcgctc caaccagctg ccgggcgatt
     2221 ccaccaagtc ggtagtcgcc acggcgtcca cgcgtcgctg cgtgcggcgt ttcagcgccg
     2281 gtgccaggca ctcgccgacg aaggccgatg tgccggctcc ggtcaatacg atacgcaagt
     2341 cctgccgctc gagcagcgga tcgagaaaag cccgcaacgc ggcggcctcg tgcatcatca
     2401 ggcgctcgat gtccgcccac accgccggtt gctgttggat ttcgcgcgcc gtccaaatcc
     2461 cgcccgccgt ttcgagttcg gccagcgggt gtgccagata gttcattcgt gtcctcgatc
     2521 cgtgacggcg ctgccggtgg cacctgcagg ccggcatgcg tagtcgtagt gacgcagcac
     2581 ctgctcgacg gcgtccagaa ccagcgcgcg cggatcgctt gtcaagcgcc cctcgcggat
     2641 tgcggtgtac tgcaccggca gatactggct gatcagcgtc aggggaacgc ggctggccgc
     2701 aaggcgcttg aggagctgcg tacacgcccg ctcgaccacg ggcgagctcc agtagtacct
     2761 gatgcggtcg ctcaagctga actgcaggtc gaaatcctgc cgctctcggt ccatgtaata
     2821 gccgctccag tggcgggggt tgcgtctcat ctcctcgatc atcgtatcct tgagcgacgc
     2881 atccg
//
LOCUS       DASWZT010000108         2673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_24985, whole genome shotgun sequence.
ACCESSION   DASWZT010000108 DASWZT010000000
VERSION     DASWZT010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24985"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1525
                     /locus_tag="VGE92_02795"
     CDS             <1..1525
                     /locus_tag="VGE92_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312772.1"
                     /translation="AMTDYARSWCGVLMAAACFPACAFGLEYWTYSYQGIEVTADRTE
                     RAKTIAHNLHRLDLAITRVLDVSQVSAWRTPTVVYSVPTATFSRLRGKKDDTTSLFLS
                     TPFENLIVIDAGEDRSDSSLFNPYFGYTGSVLVSAYTFRYPRWFIYGLSELFAASKMN
                     GDTVTIGSVNAQRGQTLVQSRNPIPMRVLMAIQEGDPQLKDADFKNLYEAEAWLLVHL
                     VVLEGKYRASFFHYFALRDQGEDEAKAFAASFNVTYEDLDKMLHDTLRTGKVETIKVS
                     VADEKDAAEPARLTAAQAAGRLAQLASQIDPQIDDAEKMANEAVSIDPKNEDALYALA
                     RAQMRRADYAASFQAAERLCSLEALTQKSDSRCGILFYRFAEGARANKASLGADQANL
                     LERSQQYFDKAITLDPNDFVAWDGMAKALTAIGNVQYAQAFLPRAAQALSKQVRNSDL
                     SRSLANLCGATGDLRAALNYAVMWQASALSPESRDAASEYVSRLRASLASSDLKRAP"
     gene            complement(1532..2365)
                     /locus_tag="VGE92_02800"
     CDS             complement(1532..2365)
                     /locus_tag="VGE92_02800"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEY1312773.1"
                     /translation="MTHVRPSIADKRRAFHKLHTGGCFVIPNPWDVGSARFLQGLGFK
                     ALATTSSGFAWSHGHPDGGMSRDRVLEHLAEMVEATDLPINADFEHGFAADAAGVAES
                     VRLAVETGVAGLSIEDSTGDAAHPLFEPQDAVERIRAARKAIDKAGGDTLLVGRAECF
                     LVGRPDVEFAIERLKSYADAGADCLYAPGIRTPEHIQAIVAGVAPKPVNLLMGWHGKL
                     SVEAIAALGVRRISVGGALARSAWGGFIRAAKLLLDEGKFDGFADAAPGVDLDRIFSG
                     G"
     gene            complement(2481..>2673)
                     /locus_tag="VGE92_02805"
     CDS             complement(2481..>2673)
                     /locus_tag="VGE92_02805"
                     /inference="COORDINATES: protein motif:HMM:NF014856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HEY1312774.1"
                     /translation="GTRRDGESIVTAGGRVLCAVGLGDSVAAAQAQAYDLVHAIHWNL
                     VQYRRDIGYRAIERESPDP"
BASE COUNT          523 a          827 c          829 g          494 t
ORIGIN      
        1 ggctatgact gattacgccc gcagctggtg tggcgttttg atggcggccg cttgcttccc
       61 ggcgtgcgcc ttcgggctcg agtattggac ctattcctac cagggaatcg aggttactgc
      121 ggacagaacc gaaagagcga agacaattgc gcacaacctg catcggctcg acctggccat
      181 aacgcgcgtc ctggatgttt cccaagtctc ggcttggcga acacccaccg tggtctattc
      241 ggtacccact gcgacgttct cgcggctgcg cggcaagaaa gatgacacca cgtccctgtt
      301 cctgtcgacc cccttcgaga acctgatcgt gatcgacgcc ggcgaagaca ggagcgacag
      361 cagcctgttc aacccctatt tcggatatac cgggagcgtt ctcgtaagcg cctacacgtt
      421 tcgctatcca aggtggttca tctacggact gtcggagtta tttgccgcct ccaagatgaa
      481 tggagacact gtgaccatcg gttcggtcaa cgcgcagagg gggcaaaccc tggttcagag
      541 cagaaatccg ataccgatgc gggttttgat ggccatacag gagggcgatc cgcagctcaa
      601 ggacgccgac ttcaagaatc tctacgaagc tgaagcctgg ctcctggtgc atctcgtcgt
      661 gctcgaaggc aaatatcgcg cgagtttctt tcactatttt gcgctacggg atcagggcga
      721 ggacgaggcg aaggcgtttg cggccagctt caatgtcacc tatgaggatc tcgacaagat
      781 gcttcatgac accctgcgca ctggcaaagt tgagacgatc aaagtaagcg ttgcggacga
      841 aaaagacgcg gctgagcctg cgcgtttgac ggcggcgcag gccgcggggc gattggcgca
      901 gttggcttcg caaatagacc cgcaaatcga cgacgccgag aaaatggcga acgaggcggt
      961 cagcatcgat cccaagaatg aagacgcgct gtacgccctg gcacgcgccc aaatgcgtcg
     1021 tgcggactac gccgcctcgt tccaggcagc agaacggctg tgctccctcg aggcgctgac
     1081 ccaaaaaagc gattcgcgtt gtggaatctt gttctatcga ttcgcggagg gcgcccgcgc
     1141 gaacaaagcc agcctcggcg ctgatcaagc caatctcttg gagcgctcgc aacaatattt
     1201 cgacaaggcg ataacgctcg atcccaacga cttcgttgcc tgggacggta tggcgaaagc
     1261 acttacggcc atcggcaatg tgcaatatgc gcaggccttt ctgccgcgcg ccgcgcaggc
     1321 tctttccaag caagtgcgca atagcgatct gtcgcgatcg ctcgcgaatt tatgcggggc
     1381 gaccggcgac ttgcgcgcgg cactgaatta cgccgtcatg tggcaggcta gcgcattgag
     1441 tccggagtct cgcgatgcag cttccgagta cgtctcgcgg ctgagagcgt cgctcgccag
     1501 cagcgatctg aagcgcgctc cttgagcgcc gtcaaccgcc gctaaaaatc ctatccaagt
     1561 ccacgcccgg cgccgcgtcg gcaaagccgt cgaatttgcc ttcatcgagc agcagcttgg
     1621 cagcgcggat gaagccgccc caagcggagc gcgcgagcgc gccgccgacg ctgatgcggc
     1681 gcacgccgag cgccgcgatc gcctcgacgc tgagttttcc gtgccagccc atcagcaggt
     1741 tcacgggctt cggtgcgacg cccgcgacga tggcttggat gtgctccgga gttcggattc
     1801 ccggagcata gaggcagtcg gcgccggcgt cggcatagga cttgagccgc tcgatggcga
     1861 actcgacatc gggccgtccg acgagaaagc actccgcacg gccgacgaga agcgtatcgc
     1921 cgccggcttt gtcgatggct ttgcgcgccg cgcgaatccg ctcgacggca tcctggggtt
     1981 cgaacagcgg atgcgccgcg tcgcccgtcg agtcttcgat cgagagtccc gcgacaccgg
     2041 tctcgacggc gagccggacg ctttcggcga cgccggcggc gtcggctgca aaaccgtgtt
     2101 cgaagtccgc gttgatcggc agatccgtgg cctcgaccat ctcggccagg tgctcgagca
     2161 cccgatcacg ggacattccg ccgtccgggt ggccatgcga ccaggcgaac ccggagctgg
     2221 tggtggcgag cgccttgaaa ccgaggccct gcagaaaacg cgcgctgccc acgtcccagg
     2281 ggttcggtat cacgaagcag ccgcccgtat gcaatttatg aaatgcgcgg cgcttgtcgg
     2341 caatgctggg gcggacatga gtcatcgcaa actccaggtg gctgttgcgc gctattgtgc
     2401 gcgccgcgct gtgctcgcgc acgcggcatc ttccatgcaa tgacatcggt cgcgatctcg
     2461 ctgttcaaga attgaacggc ctaaggatcc gggctttcgc gttcgatcgc acggtagcca
     2521 atgtcgcgcc ggtattgcac cagattccag tgaatggcat gcaccaggtc gtaagcctgc
     2581 gcttgcgcag cggcgaccga atcgcccaat ccgaccgcgc acagcacgcg gccaccggcg
     2641 gtgacgattg actcgccgtc gcggcgcgtg ccg
//
LOCUS       DASWZT010000109         4184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_5560, whole genome shotgun sequence.
ACCESSION   DASWZT010000109 DASWZT010000000
VERSION     DASWZT010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5560"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1046)
                     /locus_tag="VGE92_02810"
     CDS             complement(60..1046)
                     /locus_tag="VGE92_02810"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312775.1"
                     /translation="MLTVVLFLGSAAAIYVACEFFVNGVEWSGRKLGVGETATGTILA
                     AFGTALPESAVTFVAVVFGRDAAQKDIGVGAAMGGPLVLATISYAVVGFAMLWNHKRL
                     GRETHRIACDSQKLRRDQAWFLCIFVVKVALGLLAFSYKPWLGALFLLAYAVYLLREL
                     RGAQVLGEEDLEPLKLHPKGDPGLLLPLLQTGLALLAIALASKLFVSQLETIGIWAGL
                     SPQLVALLLSPIATELPETMNALIWVRQGKERLALANISGAMMIQATIPSALGLIFTS
                     WIFSRELIVAGIVTMLAVGLLLGLFSKNAVNARSLMPLAGLYAAFVVAIACL"
     gene            1111..2082
                     /locus_tag="VGE92_02815"
     CDS             1111..2082
                     /locus_tag="VGE92_02815"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY1312776.1"
                     /translation="MIENALQQRDVIANGIRIHFAEQGTGPLVLLCHGFPEGWYSWRD
                     QLTAFAAAGYHVVAPDMRGYGGTEAPSAISAYSIFDIVGDAVGLVAALGEQQAVIIGH
                     DWGANVAWHAALFRPDVFRAVAALSVPFRKRAPAPPLQMLRTAGLLTHYWFYLQEPGV
                     AEAEFERDPRQTLRRILFSISGDAPPQTRALTIPPGKGWLAHTIDPPELPAWLTNADL
                     DHMAAEFSRSGFRGGLNWYRNIDRNWQLTAPWAGAKILQPALFIAGSVDPVIAGGSGN
                     AALAALPTTVPGLTRLLLIDGAGHFIQQERPQVVNQALLDFVRGLAA"
     gene            complement(2071..2796)
                     /locus_tag="VGE92_02820"
     CDS             complement(2071..2796)
                     /locus_tag="VGE92_02820"
                     /inference="COORDINATES: protein motif:HMM:NF004858.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEY1312777.1"
                     /translation="MSGPVCYIKSEAVAKIVMDDGKVNVMSTAMLSALLGAFDRAEQD
                     AAIVILSSARAGIFSAGFDTKILASKQPEAIYEMVKLGAELAARILSFRYPVVAACNG
                     HAFPMGAFLLLAADVRIGADGPFKIGMNEVAIGIPVPAFALELGRERMVPAYLQRTAL
                     TGEMFAPPDAVAAGFLDRVVPPEELPAAAESAAAALCKIDFESHASTKTRLRRTAIAA
                     VRAAIEAEITLEAYRRRAVNSRG"
     gene            complement(2801..>4184)
                     /locus_tag="VGE92_02825"
     CDS             complement(2801..>4184)
                     /locus_tag="VGE92_02825"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY1312778.1"
                     /translation="EKAVPRLRHGALNLIETVGQSLAAIAPTLTPALNISVVAGLAGL
                     GCWLSYLIGTLGVVIVAASVGILAARHPEAGSYFVYIGRSFGPLAGALAGWSMISAYM
                     FTAVAVALSSAIFVADLLPAFGIQLGMLPTTLLLLVFIATVTYAAYRDVKLSSRVGLI
                     LEVISISIMILITVFFVRLQGTVVDPPQLKIAAFHFGGVFSALPFVIFSFVGFESSAT
                     LAKESANPRRNIPLAVIGCAAFSGIFFTLMAYLMVFGIGDDTMTLSKSSAPFGDVAAK
                     AGLGWASAVVYFAAIISVFACCLASINAAARLLFSMGKYRFLHRSMGLVHDTHRTPHR
                     AILLCGVLVALFCVAILPAGILNAFGYTGTFASFGFVVVYLALCCVAPMDLHRSGEMK
                     PAHVMVGIAGAALMSFVVFGSVYPVPEYPYNILPYLFFGYMASGGIWFMMLRTKSPQT
                     LASIQHDMEG"
BASE COUNT          872 a         1357 c         1268 g          687 t
ORIGIN      
        1 tctttggcgc accgccgtcg gcggtgaagt ttcgctgaat gcccgcaatg ccttgcctct
       61 cacaggcagg cgatcgcaac cacgaaggcc gcgtatagcc cggccagcgg catcagcgag
      121 cgcgcgttga ctgcattctt ggaaaatagc ccgagcagca acccaaccgc cagcatcgtg
      181 acgattccgg cgacgataag ttcgcgcgaa aaaatccagg atgtgaagat cagaccgaga
      241 gcacttggaa tcgtggcctg gatcatcatc gcgcccgaga tattggcgag tgccagccgc
      301 tccttgccct gccggaccca gatcaacgca ttcatggtct cgggcagttc ggtcgcgatc
      361 gggctcaata acaaggccac cagttgcggt gacagccccg cccagatgcc gatggtctcg
      421 agttggctga cgaacagctt ggacgccaac gcgatcgcga gcagcgcgag gccggtctgc
      481 agcagcggca agagcaggcc cgggtcgcct ttcgggtgca gtttcagggg ttcgaggtct
      541 tcctcgccca gcacctgcgc gccgcgtaat tcgcgcagca gatacaccgc ataggccagc
      601 aggaacaagg cgccgagcca cggcttatag ctgaacgcca gcaggccgag ggccaccttg
      661 acgacgaaaa tgcacagaaa ccacgcttgg tcgcgccgca gcttctgcga atcgcacgcg
      721 atgcgatgcg tctcgcgccc gaggcgtttg tggttccaaa gcatcgcgaa cccgaccaca
      781 gcatacgaga tggtcgcgag gacgagaggt cctcccatgg ccgcgccgac gccgatatcc
      841 ttctgagcgg catctcgtcc gaaaaccacc gcgacgaagg tgaccgcgct ttccggcagc
      901 gcagtaccga aggcggcaag gatggtgccg gtcgccgtct ctcccacccc caacttgcgg
      961 cccgaccatt cgacgccgtt gacgaagaat tcgcacgcga cgtagatcgc cgcagcagac
     1021 ccgaggaaca gaacgactgt gagcatgaac tgcaaaagta tacgatagca ttcgccacgg
     1081 agccgcagac attgcccggc cgggaacccg atgatcgaga acgccctgca acaacgcgac
     1141 gtgatcgcca acggcattcg cattcacttt gccgagcaag gcacaggtcc gctcgttttg
     1201 ctgtgccatg gctttcccga aggctggtac tcgtggcgcg atcaattgac ggcgttcgcg
     1261 gccgccgggt atcacgtggt tgccccggat atgcgcggct acggaggcac cgaggcgccg
     1321 tccgccatct ctgcttacag catcttcgac atcgtcggcg atgccgttgg tctggttgcc
     1381 gcgctcggcg agcagcaagc ggtgatcatc ggccatgact ggggtgccaa tgtcgcatgg
     1441 catgccgccc tgtttcgtcc ggacgtgttt cgcgcagtcg ccgccctcag cgttccgttt
     1501 cgcaagcgcg ctcccgcgcc gcctttgcag atgttgcgca ccgcgggtct tctgactcac
     1561 tactggttct acctgcagga gcccggcgtc gccgaagcgg aattcgaacg cgatccacgc
     1621 cagactttga ggcgcattct cttttccatc tccggcgacg cgccgccgca gacgagggct
     1681 ctgacgatac cgccaggcaa gggctggctc gcgcatacca tcgatccccc cgagctaccg
     1741 gcctggctca cgaacgcgga tctcgatcat atggccgcag agttttcccg cagcggattt
     1801 cgcggtggac tcaattggta ccgcaatatc gaccggaatt ggcaactcac ggcgccttgg
     1861 gcgggcgcga agatcctcca gccggctctg ttcatcgcgg gcagcgtaga tcccgtcatt
     1921 gcgggaggat ccgggaacgc ggcgctcgcg gcgctgccaa ccacggtgcc tgggctgaca
     1981 cggctattgt tgatcgacgg tgccgggcat ttcatccagc aagagcgtcc gcaagtcgtg
     2041 aaccaggcgc tgctcgactt cgttcgcggc ctagccgcgt gaattcactg cgcgccgtcg
     2101 ataggcttcg agggtgattt ccgcctcgat tgcggcgcgc accgccgcga tggccgtgcg
     2161 ccgcaagcgc gttttggtcg atgcgtgcga ctcgaaatcg atcttgcaaa gagccgccgc
     2221 cgccgactcc gctgccgccg gcagctcctc gggcggcacg acgcggtcga gaaagccggc
     2281 ggccaccgca tccggtggcg cgaacatctc cccggtcagc gcagtgcgtt gcaaataggc
     2341 cgggaccatc cgctcgcgcc ccagttccaa agcgaacgcg ggcaccggaa tcccgatcgc
     2401 cacctcgttc atgccgatct tgaacgggcc atccgcgccg atgcgcacgt ccgcggccag
     2461 cagcaggaac gcacccatgg gaaacgcatg cccgttgcac gccgccacca cgggatagcg
     2521 aaaggacaag atacgggccg ccaattccgc gccgagcttc accatctcgt atatcgcttc
     2581 gggttgcttg ctcgcgagga tcttggtgtc gaatccggcc gagaaaatcc ccgcgcgagc
     2641 cgagctgagg atgacgatgg cggcgtcctg ctcggcacga tcgaaggcac ccagcaaggc
     2701 gctcaacatg gccgtggaca tgacgttgac cttgccgtcg tccatgacga tcttcgcgac
     2761 cgcttcgctc ttgatgtaac agaccggccc gctcaacggc tcaaccttcc atgtcgtgct
     2821 ggatcgatgc cagggtctgc ggagacttgg tccgcaacat catgaaccag atgccgccgc
     2881 tggccatgta gccgaagaac aggtacggca ggatgttgta gggatactcg ggcaccggat
     2941 atacgctccc gaaaaccacg aacgacatca gcgcggcgcc ggcgattccg accatcacgt
     3001 gagcgggctt catctcgccg cttctgtgca aatccatcgg cgcgacgcaa cacagggcca
     3061 aatacaccac gacgaatccg aagctggcaa aggttccggt gtagccgaac gcattgagaa
     3121 ttcccgccgg gagaatcgct acgcagaaga gcgccacgag gacgccgcac aacaggattg
     3181 cgcgatgcgg cgtacgatgc gtgtcgtgca ccagccccat cgagcggtgt aagaatcggt
     3241 acttgcccat cgagaacagc agccgcgcgg ccgcattgat gctggcgaga cagcaggcga
     3301 acacactgat gatggccgcg aagtacacca ccgcagaagc ccagccgaga cccgccttcg
     3361 cggccacgtc gccgaaaggc gcggaactct tggagagggt catggtgtcg tcgccgatgc
     3421 cgaacaccat caaatacgcc atcaacgtga agaagatccc ggaaaaagct gcgcaaccga
     3481 ttaccgccag cggtatgttt ctgcgcgggt tcgccgattc ctttgccaga gtcgcggagc
     3541 tttcgaaacc gacgaagctg aaaatcacga acggcaatgc ggagaacact ccgccaaagt
     3601 gaaacgccgc gatcttgagc tggggcggat cgaccaccgt gccttgcagc cgcacgaaga
     3661 acaccgtgat gaggatcatg atgctgatcg atatgacttc caggatcaaa ccgacgcgcg
     3721 acgacaactt gacgtcgcga taggcggcgt aggtgaccgt cgcgatgaac accagcaaca
     3781 gcagcgtggt cggcaacata cccagctgga tgccgaacgc gggcaacaag tccgccacaa
     3841 aaatcgcaga cgacagcgcc acggccaccg ccgtgaacat gtacgcgctg atcatcgacc
     3901 atccggccag ggcgcccgcc agcgggccga agctgcggcc gatgtacacg aagtacgatc
     3961 cggcctccgg gtgccgcgcc gccaggatcc cgacactcgc ggcaacgatg acgactccga
     4021 gcgtgccgat caaataggac agccaacagc cgagtccggc aagacccgcg accaccgaga
     4081 tgttcagagc gggagtgagt gtcggagcga tcgctgccag agattgacct acggtctcga
     4141 tgaggttgag cgcgccatga cgcaaacggg gtacagcctt ttcc
//
LOCUS       DASWZT010000110         3060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_92750, whole genome shotgun sequence.
ACCESSION   DASWZT010000110 DASWZT010000000
VERSION     DASWZT010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92750"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VGE92_02830"
     CDS             complement(<1..201)
                     /locus_tag="VGE92_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312779.1"
                     /translation="MNGRDCARRTFLFLFSVQLTSTAVSAGEHLIGYSGTAAARHSNE
                     FIYGERHLLKYRDEQLAERLVLY"
     gene            430..681
                     /locus_tag="VGE92_02835"
     CDS             430..681
                     /locus_tag="VGE92_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003213503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312780.1"
                     /translation="MQITKVSSAAMAAAAAMLFTSVVPTTSFAADEATVHCTGQNACK
                     GMSECKSAKNSCKGMNSCKGQGWTSMSKAACDKAKAASK"
     gene            761..1585
                     /locus_tag="VGE92_02840"
     CDS             761..1585
                     /locus_tag="VGE92_02840"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEY1312781.1"
                     /translation="MKLAGFGLGLRPPHYESVLNEPHPIDWLEVITENYLVPGGKPLR
                     YLERMRERFPLVMHGVSLSIGGTDPIDFDYLADVRALASRIEPHWISDHLCWTGVDGR
                     NLHDLLPLPYTEEALASVVARVGQVQDALGRQILLENVSSYLTFHASEMSEWEFFREV
                     AQRADCAILLDINNIYVSSINHGFDPLCYLQAMPQERVRQFHLAGHSNLGGHLIDTHD
                     HPIVEPVWNLYRAAVARFGAVPTMIERDDNIPALHELVAELQIARDVAERGMRQAA"
     gene            1585..2367
                     /locus_tag="VGE92_02845"
     CDS             1585..2367
                     /locus_tag="VGE92_02845"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEY1312782.1"
                     /translation="MASLRQLQHDLQQYLLGDSSAITGAVVDAPPLPAAQRLAIYGNA
                     YVARLIDALGETYPVLRAVLGDEDFGALGREFIAAHPSVHRSIRWYGAELAQFCAERP
                     PFSEQPIFAELALLEWTLAEVFDAADAACVTRADLAALDPAAWSTLQLQFHPSLKRLQ
                     LCWNTVPVWRAMNRDERPPDPKCAEPTPWLLWRQNFQNLFRSMSADEAAALDAARSGK
                     TFAELCEVLTAWLPAKEVPLRAAGLLGGWADTDLLVALSKAS"
     gene            complement(2354..>3060)
                     /locus_tag="VGE92_02850"
     CDS             complement(2354..>3060)
                     /locus_tag="VGE92_02850"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY1312783.1"
                     /translation="MSPRGDSMAASRPVFIVGMPRSGTSLAEQMLASHPAVFGAGELP
                     FWNHASAIFESSRDRSLGNLGWDYLQLLQTRSAAAAHVVDKMPSNFLHLGLIHAALPN
                     ARIIHMQRNPIDTCLSIYFQDFDVAHSYATDLDNLADYYQEYVRLMSHWRAVLPKHVL
                     LDVPYEALVRDPDTWSRRMLTFVGLPWDERVLESERNSRAVQTRSRWQVRQKINSASV
                     ERWRNYQEFIGPLKRL"
BASE COUNT          602 a          903 c          955 g          600 t
ORIGIN      
        1 gtagagcacc agccgctcgg cgagctgctc gtcccggtac ttgagcaggt gccgttcgcc
       61 gtaaataaat tcgttggagt ggcgtgctgc ggcggtgccc gaatagccta tgagatgctc
      121 gccggcactg actgcagtcg acgtcagctg tactgagaac aggaacagaa acgtccgacg
      181 ggcgcaatcg cggccattca taccgagcta ttcgtgtgca gtgcaggaat ggatgcgcgc
      241 gaagctcaaa aacacgccaa atcagtccct tgcgatcgcc gcaaaggccg tatgcgcggc
      301 cgaggttgga cggctccgcc gaaaagcgca taagcataga caattgcgta ataatctcgc
      361 cgttgggctg gtctttgtag gggagcagca atatcgctgc gttgttaata actattgagg
      421 aactcaatca tgcagatcac taaagtgtcg agcgcggcga tggctgctgc ggccgcgatg
      481 ttgttcactt ccgttgtgcc gaccacctcg ttcgcggcgg acgaagcgac tgttcactgt
      541 accggccaga acgcctgcaa gggtatgtcc gagtgcaagt ccgcgaagaa cagctgtaag
      601 ggcatgaaca gctgcaaggg ccagggctgg acgtccatga gcaaggcggc gtgcgacaaa
      661 gccaaggcag cttcgaagta agacgcaacc gtcttgagaa acgggtgggg ccgggcgcgc
      721 tcgcgcgctc ggccccaccc ttgggcctct acggtaaatt atgaaactcg ccggcttcgg
      781 tctcggcctc agacctccgc actatgagtc cgtgctcaac gagcctcacc cgattgattg
      841 gctcgaagtg atcaccgaga attatctggt gcccggcggc aaaccgctgc gctacctgga
      901 gcgcatgcgc gaacgctttc cgctggtgat gcacggtgtg tcgctatcca tcggcggcac
      961 cgatccgatc gacttcgact atttagccga tgtgcgggcg ctggcgtcgc gtatcgagcc
     1021 gcattggatc tccgatcacc tgtgttggac cggggtcgac ggacgcaatt tgcacgacct
     1081 gttgccgctg ccgtacaccg aggaagcgct ggcttccgtg gtggcgcgcg tcggtcaggt
     1141 ccaagatgcc ttgggcaggc agattctgct cgagaatgta tcgagctatc tcacctttca
     1201 cgcatccgag atgagtgaat gggaattctt tcgcgaggtg gcgcagcgcg cggactgcgc
     1261 catcttgctc gacatcaata acatctacgt cagcagcatc aatcatggtt tcgatccgct
     1321 ctgctacctg caggccatgc cgcaggagcg agtgcgtcaa tttcatctcg ccggtcacag
     1381 caatctcggc ggccatttga tcgacacgca tgaccatcct atcgtcgaac cggtctggaa
     1441 tttgtaccgc gccgcagtcg caagattcgg cgcggtcccg acgatgatcg aacgcgacga
     1501 caatattccg gcgttgcacg agctggtggc ggagctgcaa attgcccgcg acgtcgccga
     1561 gcgcggcatg cgacaggcgg cgtgatggca tcgctgcgtc agttacaaca cgatctgcag
     1621 cagtacctgc tgggggacag cagtgcgatc accggtgctg tcgtggatgc gccgccgcta
     1681 ccggcagcgc agcgattggc gatctatggc aacgcatacg ttgcgcgact gatcgatgcg
     1741 ctcggcgaga cctatcccgt gctgcgcgcg gtcctcggcg atgaggattt tggcgcgctc
     1801 ggccgggaat ttatcgccgc gcacccatcg gtacaccgct cgattcgctg gtacggcgcc
     1861 gaactcgcgc aattctgcgc agagcgccca cctttctccg agcaacccat ctttgcggag
     1921 ctcgcgttgc tcgaatggac tctggctgaa gtgttcgacg ccgcggacgc cgcgtgcgta
     1981 acgcgcgcgg atctcgccgc cctcgacccg gcggcgtgga gcaccctgca attgcagttt
     2041 catccctcgc tcaagcgctt gcaattgtgc tggaacacgg taccggtgtg gcgcgcgatg
     2101 aatcgcgatg agaggccgcc cgatcccaag tgcgcagagc ccacgccttg gctgttgtgg
     2161 agacagaatt tccagaatct ctttcgttcc atgagcgccg atgaagcggc ggcgctcgac
     2221 gcggcgcgca gcggcaagac gttcgccgag ctgtgcgagg tcttgaccgc ctggctcccg
     2281 gccaaggagg ttccgctgcg agccgccggc ctgctcggcg gctgggccga cactgacctg
     2341 ctcgtcgcgc tgtctaaagc ctcttaagcg gaccaatgaa ttcctggtaa ttgcgccaac
     2401 gctcgacgga ggcgctgtta atcttttgcc gcacttgcca gcggctgcgc gtctggaccg
     2461 cgcggctgtt gcgttccgat tcgagcactc gttcatccca cggtaagccg acgaaggtca
     2521 gcattctgcg gctccaggta tcgggatccc gtaccagggc ttcgtaaggc acatcgagca
     2581 gcacgtgttt gggcagtaca gctcgccaat gagacatgag gcgcacatac tcttggtaat
     2641 agtccgccag attgtcgaga tcggtggcgt aggaatgcgc aacatcgaaa tcttgaaagt
     2701 aaatggacaa gcaggtatcg atggggttgc gctgcatgtg aatgatacgg gcgttgggca
     2761 acgcggcgtg aatgagtccc agatgtagaa aattggatgg cattttgtcg accacgtgtg
     2821 cggccgcggc cgaacgcgtt tgcaacagct gcagatagtc ccagcccaga tttccgagcg
     2881 agcgatcgcg ggatgattcg aagatcgccg acgcatggtt ccagaacggc aattcccccg
     2941 cgccgaaaac cgcaggatgc gaggcgagca tctgctcggc gagcgatgtg ccggagcgcg
     3001 gcataccgac gatgaatacc ggtcgcgacg cggccatgga gtcgccgcgc gggctcatcc
//
LOCUS       DASWZT010000111         2561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_44372, whole genome shotgun sequence.
ACCESSION   DASWZT010000111 DASWZT010000000
VERSION     DASWZT010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44372"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1163)
                     /locus_tag="VGE92_02855"
     CDS             complement(3..1163)
                     /locus_tag="VGE92_02855"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY1312784.1"
                     /translation="MSTELNFDQIRLPPECDKLRAQVRAFLAEEVDAGTFLPDRPGHG
                     DSHNRAFSERVGAKGWIGMTWPKKYGGHERTFLERFVVTEEFRVANAPVRLHFVADRQ
                     SGPILLKYAPEHIKQDILPRICRGELCFVIGMSEPGSGSDLFAAKTKATKVAGGWKIN
                     GSKIWTSNAHVADYMLGLFRTSPPTKENRRHGLTQFLVDMKAPGITVNPIIQMTGQHD
                     FNEVVFDDAFMTDDHVLGEIDGAWKQATSELAYERSGPERFLETYYVLGELVRALGSA
                     PDQRSAEGLGRLVAQLHTLRRMSVSVAGMLQTGKEPVVESSIVKDLGTVWEQKLPERV
                     RELSAFLEPESGNRVTLDEQLAFAFKVAPKLTIQGGTTEILRGIIARGLGLR"
     gene            complement(1173..2234)
                     /locus_tag="VGE92_02860"
     CDS             complement(1173..2234)
                     /locus_tag="VGE92_02860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY1312785.1"
                     /translation="MVTESDNIVAETAARIFADLADPQTVNKARDDSWKDKLWRALEE
                     AGLTLAWVPEKQGGAGASLADGFAILSVAGRFALPVPLAETLIAGWLLARAAIKAPSG
                     AMTVAPARPRDRIVLNADGTLSGSAVGIPFARDTQHIAALATGDKGASIALVKTADCR
                     VGQSENLAGDASDVVTFERVKPIRSAPAPFDVSGLMLMGAAVRSVQSAGALQTILSLS
                     VAYANERVAFERPIGKFQAVQQNLARLAGETAAALAAAGSAANTIVHSDAFDDTVFLE
                     AASAKIRTGEASAEGSAIAHQVFGAIGFTNEHVLHRFTLRLLSWRDDFDSESHWAAAL
                     GELVAKRGANQFWPTLAAR"
BASE COUNT          435 a          815 c          821 g          490 t
ORIGIN      
        1 cgttaccgca ggccaagtcc gcgcgcgatg atgccgcgca ggatctcggt ggtgccgccc
       61 tggatcgtca gcttgggtgc gaccttgaag gcgaaggcga gctgctcgtc gagcgtcacg
      121 cggttgcccg attccggctc caggaaggcg ctgagctcgc gcacgcgctc gggcagtttc
      181 tgctcccaga cggtgccgag atccttgacg atcgaggatt ccaccaccgg ctccttgccg
      241 gtctgcaaca tgccggcgac cgataccgac atccgccgca gcgtatgcaa ttgcgccact
      301 aggcggccaa ggccctcggc gctgcgttga tccggggccg accccagcgc gcggaccagc
      361 tcgccgagca cataataagt ttccaggaag cgttcagggc cgctgcgctc ataggcgagt
      421 tcgctggtcg cctgtttcca ggcgccgtcg atttcgccca acacatgatc atcggtcatg
      481 aacgcgtcgt cgaacacgac ctcgttgaag tcgtgctggc cggtcatctg aatgatcgga
      541 ttgaccgtaa tcccgggcgc cttcatgtcg acgagaaact gcgtcagtcc atgacggcgg
      601 ttttctttgg tcggcggcga ggtgcggaac aggccgagca tgtagtcggc gacgtgcgca
      661 ttgctggtcc agatcttcga gccgttgatc ttccatccgc cggcaacctt ggtggccttg
      721 gtcttggcgg caaataggtc ggaaccggag cccggctcgc tcatgccgat cacgaagcac
      781 aactcgccgc ggcagatgcg cggcagaata tcctgcttga tatgctcagg cgcgtatttg
      841 agcagaatcg gtccgctctg ccggtcggca acgaaatgca aacgcaccgg cgcgttggcg
      901 acgcggaatt cttccgtcac cacgaaacgt tcgagaaatg tgcgctcgtg cccgccgtat
      961 tttttcggcc acgtcatgcc gatccagcct ttggcgccga cccgttcgct gaacgcgcgg
     1021 ttgtggctgt cgccgtgtcc ggggcgatcc ggcaagaagg tgccggcgtc aacttcctcc
     1081 gcgaggaacg cgcgcacttg cgcgcgcagt ttgtcgcatt cgggcggcag ccggatttga
     1141 tcgaagttga gttctgtgct catcgaccga tcctaccgcg ccgccagcgt cggccagaac
     1201 tggttggcgc cgcgctttgc aaccagttcg ccgagcgccg ccgcccaatg gctttcgctg
     1261 tcgaagtcgt cgcgccacga cagcaggcgc agcgtgaagc ggtgcagcac gtgttcgttg
     1321 gtaaagccga tcgcgccgaa cacctgatgt gcaatggctg agccttcggc ggaggcctcg
     1381 ccggtgcgaa ttttcgccga cgcggcttcc aggaaaaccg tgtcgtcgaa ggcatcgctg
     1441 tgtacgatgg tgttggcggc cgagcctgcg gcggcgagcg ctgcggcggt ctcgccggca
     1501 agccgcgcca ggttctgctg caccgcctgg aattttccga tcggccgctc gaatgcgacg
     1561 cgctcattcg cgtaagcgac gctgagcgac aggattgttt gcaatgcgcc ggcggattgc
     1621 acgctgcgca cggccgcccc catcagcatc aagccagaga catcgaacgg cgccggcgcg
     1681 ctgcgaatgg gtttgacgcg ctcgaacgtc acgacgtccg acgcgtcgcc ggcgagattc
     1741 tcgctttgac cgacgcggca atcggcggtt ttcaccaaag cgatcgacgc gcccttgtcg
     1801 cccgtggcta gggccgcgat gtgctgtgta tcccgcgcga acggaattcc gacggcgctg
     1861 ccgctcagcg ttccatcggc attgagcacg atgcgatcgc gtggccgggc aggggcgacc
     1921 gtcatcgcgc cggagggagc tttaatggcg gcgcgcgcca gcagccatcc ggcaatcagc
     1981 gtttcggcga gtggcaccgg cagcgcaaaa cggccggcaa cgctcaagat cgcgaagccg
     2041 tcggcgaggc tggcgccggc gccaccttgc ttttccggca cccaggcgag cgtcaagccc
     2101 gcctcctcaa gcgcgcgcca gagcttatcc ttccagctgt catcgcgggc tttattgacc
     2161 gtctgcggat cggcaaggtc ggcaaatatc cgcgccgccg tctcggcgac gatattgtcg
     2221 ctctcggtta ccattgccgc tccggcttac tacgaatggc ggaggccatt tccgggccat
     2281 ggtgcggttc tgccggaccg ttggcaagcg tcagcatttc gcgtcgttag cgccggtcgc
     2341 agctatcttt tgtcgtgttg atcggggcta ccataccggg tagcgttgcc gtcataaaaa
     2401 gcaagaaaca tgttgtcggc cagcgatggc agcggcagct acgcagtccc ggggaggatc
     2461 aaatgggagc aagaatttcc gcgctggcga tcgctattct cgcttgtctg acgatctcaa
     2521 acgttcaggc ggccgagccc aagcacggcg gcatcctgca c
//
LOCUS       DASWZT010000112        31716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_39613, whole genome shotgun sequence.
ACCESSION   DASWZT010000112 DASWZT010000000
VERSION     DASWZT010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 31716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31716
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39613"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VGE92_02865"
     CDS             <1..380
                     /locus_tag="VGE92_02865"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY1312786.1"
                     /translation="FKQINDSFGHQAGDACLRAIIQPIHAELRQSDVIGRYGGEEFVV
                     ILSSADAAAANPIAQRILAGVSGVRVEGFGAPIRLTCSIGIAASDTLGVWGEHLLAQA
                     DAAVYVAKRLGRNQAYVAVPLTP"
     gene            569..1237
                     /locus_tag="VGE92_02870"
     CDS             569..1237
                     /locus_tag="VGE92_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312787.1"
                     /translation="MRESTDRVERALRDDTDKEHLVAHRVLESPKSWQLWESEHSSLM
                     RQVADYSVLRTQAAALRHTALRLIHGKALFEYLRKNAVRGDARSMILHHFYPTRGYHS
                     AVIAEHSGYVRKACSYLCAGHVGSGVVQDPAFVDAMQYYEELYGSYFNLYCRTLFPTN
                     GVESASEISLLPLLKHQLNEWRWMILNPRQSLPRVNRERDMRKSTGETQRLRTLQMPS
                     RAGA"
     gene            1358..1918
                     /locus_tag="VGE92_02875"
     CDS             1358..1918
                     /locus_tag="VGE92_02875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY1312788.1"
                     /translation="MTLSVLQRIAGGDSSAVRECIEQYGALVWSLARRLSRSPSDAED
                     ATQEIFLDIWRSAGRFDASQGSDKVFIAMIARRRLIDRLRKVSAEPPMDPVEALETVH
                     WADPGTASETSLEAEQATRALAELRPEQRQVLELGLLHGLSQSEIATRLQMPLGTVKS
                     FMRRGLIKVREYMNLDAEGVSVGAKV"
     gene            1918..2787
                     /locus_tag="VGE92_02880"
     CDS             1918..2787
                     /locus_tag="VGE92_02880"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY1312789.1"
                     /translation="MSAPGAQNQLDEATIDLLIKQVTEGLAPAEQRELDVLDSALASS
                     NLQRFERAAAAVALAGSARAEPLPAALKSQIEQQAQSFFDRGNARAATNVVDLGAARP
                     APRASATSGAYGWLAAAACLVLALFAWNRTPPPAPVAMAPPAPVVAPPVEKPPAPPTA
                     AEERAALLAKSDALKITFGATKDPAAAGVSGDVVWDPATQRGFLRLVGLAANDPAVHQ
                     YQLWIFDGGRDKRYPVDGGVFNIPAAAGEVVIPIHAALPVLSAKAFAVTVEKPGGVVV
                     SGREHVVVLGAAS"
     gene            complement(2814..4406)
                     /locus_tag="VGE92_02885"
     CDS             complement(2814..4406)
                     /locus_tag="VGE92_02885"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein D"
                     /protein_id="HEY1312790.1"
                     /translation="MVGRRSILKSAVVLAAGRLAYPGFAFGAPAQSQPFDYAWLKGQA
                     RKLASNAFQPSQDALPPAMAKLSYDQFQSLRFRTDHALWANAGLAFRLQFFHVGRSFT
                     EAVHLFEVVDGQAREIVYDPAMFEFDKAGIDPNLMRDHAGFAGFRVQFVTDWKADVAA
                     FLGASYFRAVGGDTRQYGLSARALAVDTAFPRPEEFPRFTSFWFERPAKDSGTLTLYA
                     LMDSPSVAGALRFQIAPGGTQAMDIDSALYPRKPIERLGVAPLTSMFFYGENDRRGAN
                     DWRPEIHDSDGLSMWTGAGEWIWRPLTNPAQLHFSSYLDDNPRGFGLLQRDRDFDHYQ
                     DDGVYYDRRPSLWVEPKSGVSGGWGKGSVQLVEIPTVDETFDNIVAFWNPAEKTKPGQ
                     ELLFSYRLYWGTRMPFSSPLAQTIATRTGIGGTVGQKRQYYSWHFAVDFAGGELGALA
                     KDAPVEAVISVSRGQTEHVTAHYVQEFHGYRALFDVRPPDDNVEPIDMRLFLRVAGKP
                     LTETWIYLWTPPSLKERKAVIG"
     gene            complement(4420..6558)
                     /gene="mdoH"
                     /locus_tag="VGE92_02890"
     CDS             complement(4420..6558)
                     /gene="mdoH"
                     /locus_tag="VGE92_02890"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF003958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HEY1312791.1"
                     /translation="MDLQLKAAVAAPRLTSGGMPPESPLHMPAQDLRYKPPRRARAGR
                     GAAVLYARFILITTSVGVTTYGVYQMLQVVRFASMTFLQGLMIFFFAISLGWIAFAAG
                     SVLAGASKRRDPVPRPDGRASLTALVMPIYNEDPLRTTSALRAMAEALAQINAQQSFE
                     IVILSDSTDADAWIRETLSVDALRKSLSTVMPVWYRRRWQNIARKSGNLEDFVTRWGG
                     RYDHMIVLDADSLIDAATLQRLVQSMQADPNLGILQTAPQLIGATTFFGRLQQFAACV
                     YGPVITRGLSAWSGDSCNYWGHNAIIRMAAFAENCGLPKLSGRKPFGGFILSHDFVEA
                     ALMRRTGWKVRMETDYHGSWEESPPSLIDVAVRDRRWAQGNLQHMKIIGTAGLSFTSR
                     MHLGVGIMSYLSSPLWLVMLGIGFALAVQSHLIRPEYFNHDFQLFPTWPRFNVELMMA
                     LFWFSMVILLIPKMLGLVHALLSKRIRRGSGGVIGVAASFLLEVVLSALYAPVLMLIQ
                     SRHVFEVFLGRDSGWKPQRRDGGGTSWADAWHYHKRHMLLSCMTAVIVWFLSPPLLAW
                     LSPALLGLFLAVPLSRASGSESLGRLLARLALLRTPEEVATPPLVARRQELLRLAKPA
                     PRDGLRHLARDRDSRLAHINGNLARPPDPRGQPDPYAFTAEQKLKDARSLDEALAWLT
                     PTERVEVAGDARLLNQLALLPDAEHPLFLI"
     gene            complement(6543..8069)
                     /locus_tag="VGE92_02895"
     CDS             complement(6543..8069)
                     /locus_tag="VGE92_02895"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HEY1312792.1"
                     /translation="MPVSIANTLLVLALTLTAVAAHGANAPAPEKPPATYPFTFDTLL
                     ADAKRRATTPYAPQRNRLPAALDKLSPEQYRSIRFNPDADIWRAEQLPFRLELLRAAN
                     NLQTAVTVSTVQDGIAEDLVATPAMFDLQSTFPGQLGKVALPVSGFRVRGRINSNKVW
                     DEFLVFQGASYFRAVAKNLLYGLSARGLAINTAEPSGEEFPAFTHFWIERPAAHSTAI
                     VIYALLESESTTGAYRFTVQPGVETAMDVDLTLFPRSDIRVAGIAPLTSMFLFDETNR
                     GHLDDYRPEVHDSDGLQIEMKSGEQIFRPLANPIKLQVSSFTTQQPLGFGLVQRSRQQ
                     SDFQDFDAQYERRPSAWVEPQGDWGPGSVELVEIPSGRETNDNIVAFWRTAQPLTAAH
                     PAHFAYRINWSAEPAQPKGLAKTIATRSGASLDGKRRVFLLDFVGAGDKVDGLRLDLS
                     ASAGKISNAALMANSALHGLRASFEIDPRDAELIELRMRIMRGDRPVTETWLYRWTSS
                     "
     gene            8151..9104
                     /locus_tag="VGE92_02900"
     CDS             8151..9104
                     /locus_tag="VGE92_02900"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HEY1312793.1"
                     /translation="MAAHAPSGQEPQSARAVLMVRPASFGFNAETAASNAFQQAPRSA
                     DSATQTLALNEFDGLARALQNAGIEVLVAPDSDQPRKPDAIFPNNWLSFHFDGTVVLY
                     PMLAANRRSERREEILGQVVREGRFRVARTVDLTHREAEGKFLEGTGSVVLDRANRVA
                     YASLSARTDLDVLGEFAQLLDYELITFETRDVHAAPVYHTNVVMAIGSRFAVVCGAAI
                     AQPLHRDAVFNRLRAAGHEIVDITLPQMCNFAGNLLELAAAGGPVIALSTTAWRSLKT
                     AQRRMLESHAALVPANIPTIERIGGGGVRCMLAELHLPKRL"
     gene            9112..9756
                     /gene="plsY"
                     /locus_tag="VGE92_02905"
     CDS             9112..9756
                     /gene="plsY"
                     /locus_tag="VGE92_02905"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEY1312794.1"
                     /translation="MLELGVKLLIGYLLGTLLGSLILGRLRGVDIRGLGSGNAGATNA
                     LRTQGKLFAFLVLLIDVAKGALAVLWLPTATLPAVGIDPDVPREWLTLGCGLAAIVGH
                     VYPVWFSFRGGKGAATVVGVIAAVELRLLVPLLASWFIVLLLSGYVSLASMLSGVALV
                     VAVCVLEPGNVPLLVFCTAIALFIIYTHRSNISRLRAGQEHRVQRLWLFRSRAA"
     gene            9729..10742
                     /locus_tag="VGE92_02910"
     CDS             9729..10742
                     /locus_tag="VGE92_02910"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY1312795.1"
                     /translation="MAVSIASRLAHPPLLVLLADGRQHSGERLAQALNVTRASVWKAV
                     ERLRALGLEVQAAPRRGYRLPNPVELLDVRRIGAEFDPLRVARLRNLELLFDVDSTNS
                     RLLAAAAPPAGSADACLAEFQHAGRGRRGRRWIAPFGGSVALSMAWSFAGGAQLAALS
                     LAAGVAVWRALARAGARGVTLKWPNDIWFQDRKIGGVLTELRTESGGPVHAVIGVGIN
                     VALGAAARRKIEASGVQVAALADACAAAPSRNLLAGALLDELLSMLVEFERAGFAAFR
                     ENWMELDALGGRQAKVLLGPMVISGTARGVDEDGALLLETAHGTQRFVSGEASLRVQE
                     GDP"
     gene            10739..11587
                     /locus_tag="VGE92_02915"
     CDS             10739..11587
                     /locus_tag="VGE92_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312796.1"
                     /translation="MTLLKGLVLCLVLANVGYFLWARGIDTAPQAPSQAAPGSLKLAS
                     EAVQGNLASGTAAGGAIAGPKGVAANSSATNNGVAANGAVTADNGATANSAPSAVSGA
                     PDAVVADAGEAGKPALLNNVQRCISVGPFRDVAEAAHAATTLRGGGYDPRQRVADGDV
                     WAGVWVYLPLPAARAAGDQILAKLKAGGIDDALEMPGPTEGSVISLGLYSEPKRAQAR
                     VAQAQALGFNPGVADRKRTGNVYWIDIDLKPTDSLLNPADLQGESGRIVRLEVRACPS
                     AAGATP"
     gene            11584..11955
                     /locus_tag="VGE92_02920"
     CDS             11584..11955
                     /locus_tag="VGE92_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312797.1"
                     /translation="MNAREASLKVFGGIGAVFGYGCLLAFLYLVGLQAYRWFRDGEWT
                     HIGVNEGLRAGLARCCVKEGDTGRLADFVQWLDAPVNWLGLHKVFEVLPASLAIFAVS
                     IAGNCLFIYCRDRLDGREIQP"
     gene            11984..12757
                     /locus_tag="VGE92_02925"
     CDS             11984..12757
                     /locus_tag="VGE92_02925"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEY1312798.1"
                     /translation="MNDPILTLLRLKTLLRSVILPPTGPLLLAVLGIVLIKRRPRLAR
                     ACLIAAIGTLWLLSTPVISDALVRWVERYPPLVISSTGGAQAIVILGGGGERAFAPEY
                     GGPAADPTLLERLSYGAYLARNTALPILVTGYHIEAMAMQASLERNFDLTPRWVDADA
                     YDTFENATNSVQLLKTAGIHRIILVTHATHMWRAVQEFNAAGIEVVPAPVGISAEREP
                     GLGRYIPNPEGLLRANVALYEVLGEQVRNVLAAVHLRRH"
     gene            12859..13071
                     /locus_tag="VGE92_02930"
     CDS             12859..13071
                     /locus_tag="VGE92_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312799.1"
                     /translation="MRVLQIVGAILIAGGLFVLIKSPSYSSDKSVFKIGDVEAKVAQQ
                     HAISPWIGGAALAAGLVLIVTGARRP"
     gene            complement(13092..13955)
                     /locus_tag="VGE92_02935"
     CDS             complement(13092..13955)
                     /locus_tag="VGE92_02935"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF042973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-polyphosphate phosphotransferase"
                     /protein_id="HEY1312800.1"
                     /translation="MHIKSSLFRVPEGARVDLREWPTRAAPAYASNSDYADLLRKHVA
                     GLSEQQQLLYASNRYAVLLIFQAMDAAGKDGAIQHVMSGVNPQGCQVFSFKHPSAVEL
                     QHDFLWRTTRDLPERGRIGIFNRSYYEEVLIAKVHPEILRGEGLASTNEKTIWDQRYR
                     SIVDLERHLQRNGTRIIKFFLHLSKDEQRKRFIDRIDKQDKNWKFSLADIEERKYWKD
                     YMRAYEQCLAATSTKRAPWYIVPADDKLNTRLIVSRIVLDTLAALKLSYPKTSAARRR
                     ELKSLRKRLAK"
     gene            14066..14962
                     /locus_tag="VGE92_02940"
     CDS             14066..14962
                     /locus_tag="VGE92_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY1312801.1"
                     /translation="MYSRLAAGVLLLTVIGLANAHAAPFELPPLNSPASSEHHPGKVV
                     WAELVTPDLAAAEHFYASLFGWTFQTVHSGASEYAVAAVDGRPVGGLFQKSVAAGEHK
                     QSAWLTFIAVSDVDAAKRAAVGQGAKIVSDTRDYPLRGRQAVLADPEGAVFAILASQS
                     GDAADFLAAPGEWIWSSLLSQDAAAEAAFYQTLFAYDVFDLASDDDSEHLILSSDGFA
                     RGSVNSFPAGAARRHAHWLNFVRVDNTADSVAKAVALGGRILVEPHPDRHGGQIAVLA
                     DPAGAPFGLMEWTPRDSKVEPK"
     gene            14959..15249
                     /locus_tag="VGE92_02945"
     CDS             14959..15249
                     /locus_tag="VGE92_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312802.1"
                     /translation="MTRARHRWLTGGLAVLALTLLHACAVTGVGVDGSVGVGYDAGYY
                     EPWGYDYGGWGGGYRVGPGRGGERRGGHAPPRYRPAPSGRPSPSIPGRPRGR"
     gene            15337..16635
                     /locus_tag="VGE92_02950"
     CDS             15337..16635
                     /locus_tag="VGE92_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1312803.1"
                     /translation="MPDPTTRPDARQTRRILFASLIGTTIEFFDFYIYATASVLVFPK
                     LFFPKSDPNTAMLQSFATFALAFFARPVGSALFGHFGDRIGRKATLVTALLTMGVSTV
                     AIGLLPTYATIGVSAALLLALCRLGQGLGLGGEWGGAVLLATENAPPGKHGWYGMFPQ
                     LGAPLGFLCSTGVFLLISNRLSEAQLFSWGWRVPFLASAVLVLVGLYIRLQLVETPAF
                     KLSLAQGERVRVPFLVVIARHPLRLLLGTLGSTCTFVVFYLMTVFALGWATHSLGFAR
                     QQFLILQMIGVLFFGLTIPLAGIIADGVSGRRMLMIATVLIIIFGFAFQPLFSAHNTA
                     SVLSFLVLGFALSGLTYGPLGSALATLFPTAVRYTGTSLAFNFAGILGASLAPSIATS
                     LAERYGLAYVGYYLSSAGAVSLLALLMIKNDEPRVTGECK"
     gene            16632..17405
                     /locus_tag="VGE92_02955"
     CDS             16632..17405
                     /locus_tag="VGE92_02955"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY1312804.1"
                     /translation="MIRTTPAVRLAATLLFTACLSSGLEANAADAYDAAVAHAGRSAA
                     DIKRDQLDHPADILRLSGIKPGMRVADVLAGDGYYSELASYVVGPTGKVFLINNTAFD
                     KWSDNLPARLQGNRLGNVEHQTLDLDQMNLKPGSLDAILLVKVYHDLYWVDPEGVWPK
                     VDVGGVLNQLARALKPGGVLLLVDHSAKPGSGNSVAGSLHRIDEQFAIKDFESHGFKV
                     VATSDLLRRPDDARDLITYKGPAVGKTDRFVVVFRKGAA"
     gene            complement(17437..18492)
                     /locus_tag="VGE92_02960"
     CDS             complement(17437..18492)
                     /locus_tag="VGE92_02960"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEY1312805.1"
                     /translation="MARNLVFALGALGLWCAAATASTDSSPLPAPVYTVTGTWSLGGA
                     GGWDYLALEASGARLFVSRADRVDVVETVSGKITGTIANTAGVHGIAFAPALMRGYAS
                     DGRSDSISVFELGSLRLIQEVPVSGKKPDAILYEPRQNRIITANGDSSNLSVVDAGTL
                     QVIATVPLPGPPEFMTTDDAGTVFVNIETDPGKLVAVDAKSMTVKATWPLAGCANPTG
                     QALDVAHHRVFSVCANQIMAVTDSTSGKAVARVVIGSGPDAAAFDPDLSMVFSSNGMD
                     GTLTVIHQETPDEYRVVATVTTQVSARTMVLDPATHKIYLAAAKLAATPPATDAQPHP
                     RPAIVPDSFSILTVQPK"
     gene            18603..19979
                     /locus_tag="VGE92_02965"
     CDS             18603..19979
                     /locus_tag="VGE92_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1312806.1"
                     /translation="MQRDYLGNPLGAQSATTARAIDDFIEGLLAYETRAERVLIAADE
                     EPASCMANVYSGLLWMLLEAPAGAANAAKYLAAAERTAADATLREQLNTAMLRAWVDD
                     DLLQTLRLCERIADEFPRDLVMLKIHQYLEFNRGNPAAMLRVALRALRAAPDVAYVHG
                     MAAFGYEQCHLLNEAEAAARTALDMRRKEPWAQHALAHVSLTRGLIDEGAEFLEQMAD
                     TWSNLNSFMVTHLWWHLALFYLSQGRYRRVLELYDRHCWGVAKDYSQDQIGAISLLAR
                     FEIAGIDVGPRWRELGGYLVARAQDTTLPFLTLQYLYGLARAARPEADSLLAAVRRRA
                     ETAPAFARPAWREVALPACEGLYAHARGNYDKAWAYLVVSVPRMAEIGGSHAQRDLFE
                     QILLHAAVKDGRFSAAQQMLELRRLADPRGVPVNTGLAAVYVELGLPQLAEQAQARAM
                     FTRRQHPA"
     gene            19996..21276
                     /locus_tag="VGE92_02970"
     CDS             19996..21276
                     /locus_tag="VGE92_02970"
                     /inference="COORDINATES: protein motif:HMM:NF009558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine
                     deacetylase/succinyl-diaminopimelate desuccinylase family
                     protein"
                     /protein_id="HEY1312807.1"
                     /translation="MRNDPVDGIFKTIENRRDDLIELTCDLIRFPSVNPPGEAYRPCA
                     QFIGDRMRARGFDVEYVRAAGTPGDCDAYPRLNVIARRPGAAPGPCVHFNSHIDVVQQ
                     GAGWTVDPFAGVVQNGRVYGRGSCDMKGGLAASIIALEALIDGDAKLPGTLEISGTVD
                     EESGGYGGVHFLAERGWFSPPRVDHVIIPEPLNVDRVCIGHRGVWWAEIETHGRMAHG
                     SMPFLGDCAVRHMHAVVERFERELYPKLAARRTVMPVVPPGARHSTLNINSIHGGLAE
                     APGYPAALVPDSCRMVIDRRFLIEENIDGVKGEVAELLERLAAERAGFRYRMSDIFEV
                     QPTMADRDGPVARATAAAIRRVMSCEPEFICSPGTYDQKHIDRVGKLRDCIAYGPGIL
                     DLAHQPDEYVVIDDMLNAAKVMALAARSLLYRSA"
     gene            21385..21738
                     /locus_tag="VGE92_02975"
     CDS             21385..21738
                     /locus_tag="VGE92_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312808.1"
                     /translation="MKFGNSFGNSRTAARPEPAKMPSAVAQRSTPWHAVSIVAKNESC
                     DAARALRNARFLSAQAPRLPLAACTLGGACPCGYKHHSDRRGQPRRKDEITGLKQTNK
                     SPVERRVSPDRRAAD"
     gene            complement(21743..22738)
                     /locus_tag="VGE92_02980"
     CDS             complement(21743..22738)
                     /locus_tag="VGE92_02980"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEY1312809.1"
                     /translation="MITDISGLEVGHATDEHVRSGVTALLCGDGWSAAVDVRGGAPGG
                     RETDALAPENLIGRAHAIVLAGGSVFGLAAADGVAAALSVKGIGLQLRTGSPRIPIVP
                     AAVLHDLANGGDKQWGLEPPYRGLGVRALAAAGGDFALGAHGAGRGAMAGVVGGGVGS
                     SSLDLGERLIVGALAAVNSVGSALMPDGKSYWAWPFELDAEFGGAGAPAGQIDASDPA
                     PDASRLVSLGRLQAGANTTLGVVACNADLSTLECKRVAMMAHDGIARALRPAHTPFDG
                     DTIFSIASGELALRSEPPRAVQIGRIGSAAADCLARAIARAVHFSCTFGATHRPR"
     gene            complement(22770..23525)
                     /locus_tag="VGE92_02985"
     CDS             complement(22770..23525)
                     /locus_tag="VGE92_02985"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEY1312810.1"
                     /translation="MILVIDVGNTRLKWAWLTSTGLSDQRAVVHRDAKIGMWTAALFE
                     SGETPQRVLVSNVAGPVMAKTLSNLAKKKFRVKVEFVTASAQFNGLTNGYLDPSLLGA
                     DRWLALIGAWTKARSALCVVDAGTAVKVDGVDANGQHLGGLIVPGIHMMREALMNKTS
                     DIAKAALHSTPSLAGVLANNTLGAVSRGAVFALAGMADRAAEVIEQSTGTKPKLFITG
                     GDSGMITGTMRSQGEIVPDLVLQGLAVIAAQPR"
     gene            complement(23589..25520)
                     /locus_tag="VGE92_02990"
     CDS             complement(23589..25520)
                     /locus_tag="VGE92_02990"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY1312811.1"
                     /translation="MRTLNLYPVLAGLAPALLVSFQALAAAAPATPPAAAPAAIAAPL
                     TGQRVIQVLDQAIDWFRTLGIQQQAANEPSDLLILYDNRQIANQVIALAFEIARADVD
                     VLAKQPATKDSGGTAAATQLSQLESKYAAQGAAVHSELESVQTQLAKSAAEQRNVLQA
                     KISELQGELELIDARTTLLATMSSLTSQSDAGGFSASGLRSQIDAMAVTLSATPAPAN
                     AAASGGGAALTAAAGPAQQTPAAASTPLNSISGIAAARFGIWDLAANVIRLSEKAATV
                     DEIDARAAALQNTFARIRGPIIDDLKTLSARGDAVAAQGDTTDSAALNHARDQLDLVS
                     SEFKQISALLIPLSKEGVLLNQYRRNLASWRDAIRNQYRGALKTLGVRVGILLILLAL
                     VFAAAEVWRLAVIRYIQDARRRYQFLLLRKIAFWALVVVIIGFAFASELGSIVTFAGL
                     ITAGLAVAMQSVLVSIVGYFFLIGKYGIRVGDRVQIGEVTGEVIELGLVRLHLMELGG
                     HGLLGPTGRVVAFANSIVFQVSSGLFKQIHGVNFVWREITLSLPAGIDYAAAKAKLTA
                     AVTEAVKDERAEIVQQSKEIERATSSNIGGDSQPRVQLSFSAGGADAHVRYPAHLQNA
                     AEIDERVSQSLVAVVASLR"
     gene            25607..26110
                     /locus_tag="VGE92_02995"
     CDS             25607..26110
                     /locus_tag="VGE92_02995"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEY1312812.1"
                     /translation="MFDFKQAISDVADFPRPGILFRDISPLLRDHLEVTVQALDGLLT
                     EAEWSGIDALAGIESRGFILGAALALRRGKGFVLVRKQGKLPPPVVDVTYELEYGSGV
                     LEMQRGQGRVILVDDVLATGGTMSAAADLCVRAGYRLHALIALIDLHIVANYSWRSLP
                     LRAVINY"
     gene            26139..28412
                     /locus_tag="VGE92_03000"
     CDS             26139..28412
                     /locus_tag="VGE92_03000"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEY1312813.1"
                     /translation="MKSALLGCLLTAFCASACAGVRVIVGPTPIKGGEAKSAGDITVV
                     NEKLAFALAVQSPVPYGVPRGAIIDVAPVVNGEIGRDCVVFADFIPNNWSAWPNTFQH
                     IEILQRGPTRVVVRTVRDWGKVTITTLYTLAAAADSIAIRTTMHNGGAALADLLSGLT
                     LWPKGGHFFGVPGLRGVVEGSTEMALADRVVAYDEQWSVALHAPYADHIADGSLDLYR
                     LHTLAAGESRVFEGWLQVGASGDLKPVIAAEIARRHLTAGTVRGTVAASNAQPVEAPV
                     IVVEKHGKPYGWVLGRGGVFEFALAQGDYLLYATAKGYSESARIPLQVKPGAVANVDF
                     RELRPPGNIRWSVADARSGAPVDARINIEEGQKPLVEFLGRNTFFTELERRGRVDVPI
                     APGDYRFSVSSGGGFLAQSERVKLSVAPGEARAAQVALTRLFDPPARHWYAADLHHHA
                     DQAEAVTPPADLARSQLAAGLDLLFVSDHDSTVNHATLRELAERRGVAFIPGIELSAS
                     WGHFNAYPLAPDQTLAIDTGTASIDELLTAARRMGATVVQVNHPFIPYGYFTSVSRGV
                     APGGFNPSFDLIEINAAAPADDAKVLRTLWNFWNAGHRYYLAAGTDTHDVWHEESGAV
                     RTFAHIDGAVTAVAYAQALKDGHGYVTFGPLIFPSVIFGDELRVKPREPFNLGFDLQA
                     VAGLKQVELIESGRVLKTEAWTAASQQVHVDFPLTTEHATWYSLIVEDNQGHKAYSDP
                     IWVDTVAGLTPESRAAQ"
     gene            28526..>31716
                     /locus_tag="VGE92_03005"
     CDS             28526..>31716
                     /locus_tag="VGE92_03005"
                     /inference="COORDINATES: protein motif:HMM:NF037482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CshA/CshB family fibrillar adhesin-related
                     protein"
                     /protein_id="HEY1312814.1"
                     /translation="MTSIIQRAALGLVVLAGTPALTYANSCAPATGQGTAPSDYQDYC
                     WLDFTGYSDAMARAGGQPFTFTLPDGSTLSMTVQVSTNKTNPALVAHIVPSWTGSAIG
                     NKAFTGIPGQPVLYETVNGSTVQLALSNITVVPPPGGGASASYAMIAADGESTNQTEQ
                     LVFTTNGQAWTQVAQIAQGTLYPTVSGVGTNTVTETGVAGTVGSFVFASFNNPTQLSA
                     TLVGGGLQGAMFAIRYASLAANAQFSGARASASDQFTYGISTTGGQSIASGTTSGSGT
                     GPFTPAVAPTIAASYPFVVSETLAAGSTSTLANYAVSLTCTNGASGTSSTVLPTNLAN
                     RTYTFSSLQYGDAVSCVFTNTANRANLTVAKSGPTSVSAGAPVSYNVVVANAGPSDAG
                     GAFVKDPAVANFTALAVTCGSAAGGAVCPTAGLTIANLQGSGILIPTLPSGGSVTFAV
                     SGTAGTGTIVNLASVTPPSTVVNSNTTPTSSATTTVTPPPDALSTAAFPVNVAAGQPV
                     NGTVTFSNGGSGIANNTTFSMTLAANLAAATLTGLPAGATYAYAPGTGIVAFAGMPTT
                     LAAGASIGPIGVHYTQPSAGTSTVTATVSTTADSNPANNTVSVVVGGPPDAISSVAFP
                     ASVYPGQPVSGTVSFSNGGTGIATNTSFGMTLPANLAVAPTLTGLPAGAAYSYAAGTG
                     VTTLTGMPTSIAVGASLGPIGVNYLQPASGSSTVTAVVATTGDSNPSNNTATAVIGGG
                     PPDASSAAQFPATVNPGQPVNGTVTFSNGGLGVATNTTFAMTLPANLATAPTLTGLPA
                     GAGYAYAPGTGVITFTGMPTTLAVGIPVGPIGVHYVQPASGTSTVTAAVTDTGDSNPS
                     NNSVTVVVGGGAPDAQSVVAFPNAVDPGQPVNGTVTFSNGGLGVATNATFAMTLPANL
                     ATAPTLAGLPAGVTYAYAPGSGVITLTGMPTSLAAGSSIGPIGVGYLQPASGSSTVIA
                     VVTTTGDSNPSNNTATAVIGGGPPDAIATAAFPANVIAGQPVNGTVTFSNAGLGPASN
                     ASYGMTLPANLATPPTLTGLPAGASYAYAPSSGA"
BASE COUNT         5648 a         9811 c        10408 g         5849 t
ORIGIN      
        1 atttcaaaca gatcaacgat tcgttcgggc atcaggcagg cgacgcctgc ctacgcgcca
       61 taatccagcc gatacacgcc gaattgcggc agtccgacgt catcggccgt tatggaggcg
      121 aggaattcgt ggtgatccta agcagcgccg acgcggccgc ggcaaacccc attgcgcagc
      181 gaattctcgc tggagtgtcc ggcgtgcgtg tcgagggttt tggtgcgccg attcgtctca
      241 cctgcagcat cggcatcgcg gcgagcgaca cgctgggtgt ctggggcgaa catcttctgg
      301 cgcaagcgga tgccgcagtg tatgtcgcca agcgattagg acgtaatcaa gcctacgtcg
      361 cggtgccgct aactccgtag cagttcgcaa ttaaatgtcg cataatggcg acgcattaat
      421 aattcattga cagcggcctg ccataagttg atcttatgcg atgagcggcc cgtcacgaat
      481 tgggctaatt gctacccagt cgtggattat taagcgccgc aggaggatgt gaagagtgca
      541 ccgcagtgct gttagtcatg cacttctgat gcgcgagtcg accgatcgag tcgagcgcgc
      601 gctccgcgac gacaccgaca aagaacacct ggttgcacat cgcgttctcg aaagcccgaa
      661 gagttggcag ctgtgggaga gtgagcactc gagcctcatg cgccaagttg cggactacag
      721 cgtattgcgc acacaggcgg ccgcgctccg acataccgcg ctgcggttga ttcacggcaa
      781 ggcgttattc gaatacttgc gcaagaacgc cgtgcgcggc gatgcgcgca gcatgatctt
      841 gcatcacttc tacccgacac gaggctacca ttccgcggtt atcgcggagc acagcggcta
      901 tgtgcgcaaa gcctgcagct acctgtgtgc ggggcacgtg ggaagcgggg tcgtgcagga
      961 cccggcattc gtcgacgcga tgcagtatta cgaggagctg tacggctcat atttcaatct
     1021 gtactgcagg acattgttcc cgacaaacgg cgtcgaatcg gccagcgaaa tatccttgct
     1081 accgctgctc aagcaccagc tcaacgaatg gcgctggatg atcttgaacc cgcgacagag
     1141 tttgccgcgc gtcaatcgcg agcgcgacat gcgcaagtcc accggagaaa cgcagcggct
     1201 tcgcacgctg cagatgcctt ccagagcggg cgcttaacac ggttcaggcc gatttagcat
     1261 cgccgggcat gccttcctgg tatggtttgc aacataatta gccgatacgg ttgcatccgc
     1321 ccgtgttgtc gatccgaata agttctcaag gccctcaatg actctgtccg tgctgcaacg
     1381 cattgccggc ggcgattcgt ccgcggtgcg tgaatgcata gaacaatacg gtgcactcgt
     1441 ttggagtttg gcgcgacgcc tgtcgcgctc gccgtcggat gccgaggacg cgacacagga
     1501 aatctttctg gatatctggc gcagcgccgg tcgcttcgat gcatcgcaag gctccgacaa
     1561 ggtgttcatt gcgatgatcg cccggcgccg tttgatcgat cgactgcgca aagtaagcgc
     1621 cgaacctccg atggatccgg tcgaggcact ggaaaccgtc cattgggcgg atccgggcac
     1681 cgcatccgaa acatctctcg aggccgagca ggcgactcgg gcgctggcgg agctgcgtcc
     1741 cgaacaaagg caagtattgg agctcgggct gctgcatggc ctgagccagt ccgaaatcgc
     1801 gactcgcttg cagatgccgt tggggacggt gaagtctttt atgcgtcgcg gcctcatcaa
     1861 ggtgcgcgaa tacatgaatc tggatgccga aggcgtctca gtgggagcga aggtctaatg
     1921 tcagctccgg gcgcgcaaaa ccagctcgac gaagccacga tcgacttgct gatcaagcag
     1981 gtcaccgaag gactggcgcc ggccgaacag cgggagctcg atgttttgga cagtgcgctg
     2041 gcaagcagca atctgcaaag attcgaacgc gccgcagcag cggttgcgct tgcgggcagt
     2101 gcgcgcgccg agccgttgcc cgctgccttg aaatcgcaaa tcgaacagca agcgcagagc
     2161 ttcttcgacc gcggcaacgc ccgcgccgcg acgaacgtgg tcgacttggg cgcggcgcgt
     2221 ccggcgccgc gcgcgtccgc aacgtcgggt gcctacggat ggctcgcggc cgcggcatgc
     2281 ctggtgctgg cattgttcgc gtggaatcgc acgccacctc ctgccccggt ggcaatggcg
     2341 ccacccgcgc cggtcgttgc gccgccggtt gagaagccgc ctgcgccgcc gactgcggcg
     2401 gaggaacgtg ccgcgttatt ggccaagtcc gatgcgctca agatcacctt cggggcgacc
     2461 aaggatccgg ccgcggcggg cgtcagcggc gatgtggtgt gggacccggc cacccagcgc
     2521 ggctttttgc gcctcgtcgg tctcgcggcc aatgatccgg cggtgcacca atatcaattg
     2581 tggattttcg acggcggccg cgacaaacgc tatccggtgg atggcggcgt tttcaacatt
     2641 ccggccgccg ccggcgaagt cgtgattccc attcacgcgg ccctgccggt gctgtcggcg
     2701 aaggcgttcg cggtaacagt cgaaaagccc ggcggcgtcg tggtctcagg gcgcgaacat
     2761 gtggtcgtgc tcggcgccgc cagctaggcg ccccccagct aggcgccccc aagctaaccg
     2821 ataaccgctt tgcgctcctt caaggacggc ggggtccaca gatagatcca cgtttcggtg
     2881 aggggtttgc ccgccacgcg caagaacagc cgcatgtcga tgggctcgac gttgtcgtca
     2941 ggcggtcgca catcgaacaa cgcgcggtaa ccgtgaaatt cttggacata gtgtgcagtt
     3001 acatgctcgg tttgaccgcg cgagacgctg atcaccgctt cgacgggcgc gtccttggcc
     3061 agcgcgccga gttcgccgcc ggcaaagtcg accgcaaaat gccagctgta gtattggcgc
     3121 ttctggccca ccgtgccgcc gatgccggtg cgggtcgcga tggtttgcgc gagcggcgat
     3181 gagaagggca tgcgcgtgcc ccagtacagg cgatagctga acaagagctc ctggccgggc
     3241 ttggttttct cggccggatt ccagaatgcg acgatgttgt cgaacgtttc atccaccgtt
     3301 gggatctcca ccagctgcac cgagcccttg ccccagccgc cgctgacgcc ggatttgggc
     3361 tcgacccaca ggctcgggcg acgatcgtag tacacgccgt catcctggta gtgatcgaaa
     3421 tcgcggtccc gctgcagcag accaaaaccg cgcgggttgt cgtcgagata ggagctgaag
     3481 tgcaactgcg ccgggttggt cagcggccgc cagatccatt cgccggcgcc ggtccacatc
     3541 gacaggccat cggagtcatg gatctccggc cgccaatcgt tcgccccgcg ccgatcgttc
     3601 tcaccgtaaa agaacatgct ggtgagcggt gcaacgccca gcctttcgat cggcttgcgc
     3661 ggatacagtg cgctatcgat gtccatggcc tgagtgccgc cgggcgcgat ttgaaagcgc
     3721 agcgcaccgg cgacgctcgg tgagtccatc aacgcgtaca gcgtcaatgt ccccgaatct
     3781 ttcgccggcc gttcgaacca gaaggaggtg aagcgcggaa actcttccgg acgcggaaag
     3841 gccgtatcca cggcgagcgc gcgcgccgac aatccatact ggcgtgtgtc cccgccgacc
     3901 gcccgaaaat agcttgcgcc gaggaaggca gcgacatcgg ccttccagtc ggtcacgaac
     3961 tgcacgcgaa agcccgcgaa acccgcgtga tcgcgcatca gattggggtc aatgccggcc
     4021 ttgtcgaact cgaacatggc cgggtcgtaa acgatctcgc gcgcctggcc gtcgactact
     4081 tcgaaaaggt gcacggcctc ggtgaagctg cgtccgacgt ggaagaactg caggcgaaac
     4141 gccagtccgg cattcgccca gagtgcgtga tcggtacgaa accgcagcga ttgaaattgg
     4201 tcgtagctga gctttgccat cgcgggtggc agcgcatcct gagacggctg aaaggcgttg
     4261 ctggcgagct tgcgtgcctg gcccttgagc caggcgtagt cgaagggttg gctctgagcg
     4321 ggcgcgccga acgcgaatcc aggataggcc aggcggcctg cggccagcac cacggccgat
     4381 ttgagtattg agcgtcgtcc aaccatcgtt cttcttcctc taaatcaaaa acaaggggtg
     4441 ctcggcatcg ggcagcagcg cgagctgatt gagcagccgg gcatcgcccg ccacctcgac
     4501 gcgttcggtc ggcgtgagcc aggccagcgc ctcatccagc gagcgcgcat ccttcagctt
     4561 ttgttccgcg gtgaaggcat aggggtcggg ttggccgcgt gggtccggag gccgcgccag
     4621 attgccgttg atatgcgcca gccgcgagtc gcgatcacgc gccaggtggc gcaggccatc
     4681 gcgcggcgcc ggttttgcca gccgcaacaa ttcctggcgg cgcgccacca gcggcggcgt
     4741 agcgacttcc tcgggcgtgc gcagcaaagc caaccgcgcc aggagtctgc ccagactttc
     4801 gctgccgctg gcgcgcgaca aaggtactgc gagaaaaagt ccgagcagcg cgggcgacaa
     4861 ccacgcgagc aacggcggcg agagaaacca cacgatcact gcggtcatgc aactgagcag
     4921 catgtggcgt ttgtgatagt gccatgcatc cgcccagctg gtgccgccgc cgtcgcggcg
     4981 ctgcggcttc cagccggagt cacgtcccag gaacacttcg aacacgtgcc gcgattgaat
     5041 gagcatgagt accggtgcat acaaggcgga caggacgacc tccaatagga agctcgccgc
     5101 gacgccgatg acgccgccgc tgccgcggcg tatgcgtttc gacagcaagg catgcaccaa
     5161 gccgagcatc ttgggaatca ggagaatcac catgctgaac cagaacaagg ccatcatgag
     5221 ttcgacattg aaacgcggcc acgttggaaa cagttgaaag tcatgattga aatactcggg
     5281 ccgaatcaag tgcgactgca ccgcgagcgc gaaaccgatg ccgagcatca ccagccacaa
     5341 tggcgaggac agatagctca tgatgccgac gccgagatgc atgcggctgg tgaagctcaa
     5401 gcccgcggtg ccgatgattt tcatgtgctg cagattgccc tgcgcccagc ggcggtcgcg
     5461 aaccgccacg tcgatcaacg acggcgggga ttcttcccag gagccgtgat agtcggtctc
     5521 catccgaacc ttccaaccgg tgcggcgcat cagcgcagcc tcgacgaagt cgtgcgacaa
     5581 aatgaagccg ccgaacggct tgcggccaga gagtttgggc aaaccgcagt tttccgcgaa
     5641 cgcggccatg cgaatgatgg cgttgtggcc ccaataattg cagctgtcgc ccgaccaggc
     5701 actcaagccg cgagtaatca cggggccgta gacgcaggcc gcgaattgct gcaggcggcc
     5761 gaagaaggtg gtggcaccga tgagctgcgg agcggtttgc aggattccga ggttgggatc
     5821 cgcctgcatg ctctgtacca gtcgttgcag agtcgcagcg tcgatcaagc tgtcggcatc
     5881 cagcacgatc atatgatcgt agcgcccgcc ccagcgcgtg acgaagtcct cgaggttgcc
     5941 ggatttgcgc gcgatgttct gccagcggcg ccgataccac accggcatga cggtggacag
     6001 tgatttgcgc agcgcgtcga cgctcagcgt ctcgcgaatc cacgcatccg cgtcggtcga
     6061 gtcggagaga atgacgatct cgaaactttg ttgcgcgttg atttgcgcca gcgcctcggc
     6121 catggcgcgc aatgccgacg tggtgcgcag cggatcttca ttgtagatcg gcatgaccag
     6181 cgccgtcagc gaggcgcgtc catccggacg gggtaccggg tcacgccgtt tggatgcgcc
     6241 cgccagcacc gagcccgccg caaacgcgat ccagcccagg gatatcgcga agaagaagat
     6301 catcaagccc tgcagaaaag tcatgctcgc gaagcgcacc acttgcagca tttgatacac
     6361 gccgtaggtg gtcacgccga ccgaggtcgt gatcaaaatg aagcgtgcat acagcaccgc
     6421 cgcgccgcgt ccggcgcgcg cgcgccgtgg tggtttgtag cgcaaatcct gcgccggcat
     6481 atgcaacgga ctctcgggag gcatgccgcc cgacgtcagt cgcggtgccg cgacggcggc
     6541 cttcagctgg aggtccatcg atacagccat gtttcggtga ccggccgatc gccgcgcatg
     6601 attcgcatgc gcagttcgat caactctgcg tcgcgcggat cgatttcgaa actcgcccgc
     6661 aggccatgca acgcactgtt cgccatcagc gcagcattcg agatttttcc tgcggacgcg
     6721 gataagtcga ggcgcagtcc atccactttg tcgccggcgc cgacaaaatc caataagaat
     6781 acgcgccgtt tgccgtcaag actggcgccg gagcgggtgg cgatggtttt tgccagcccc
     6841 ttgggctgcg ccggttccgc agaccaattt atgcggtagg cgaaatgcgc cgggtgcgcg
     6901 gccgtgaggg gctgcgcggt acgccagaac gccacgatgt tgtcgttggt ctctcgtccc
     6961 gacggaatct cgaccagttc gacggatccc gggccccagt cgccttgggg ctcaacccag
     7021 gcgctgggcc ggcgttcgta ctgggcgtcg aaatcctgaa aatcgctctg ttgtcgcgag
     7081 cgctgcacca gcccgaaccc gagcggctgc tgggtggtga agctcgatac ttggagcttg
     7141 atgggattcg ccagcggacg aaaaatctgc tcgcccgatt tcatctcgat ctgcagcccg
     7201 tcggagtcgt ggacttcggg ccgatagtca tcgagatgtc cgcggttggt ttcgtcgaat
     7261 agaaacatcg aggtgagcgg cgcgataccg gcaacgcgga tgtccgagcg cggaaacaaa
     7321 gtgaggtcga cgtccatggc ggtctcgacg ccgggctgca cggtgaaacg atacgcgccc
     7381 gtcgtcgatt cgctctcgag cagggcgtat atgacgatgg cggtcgagtg cgcggccggt
     7441 ctctcgatcc agaaatgcgt gaacgccggg aattcttcgc cggaaggttc cgcggtattg
     7501 atcgccagac cacgggcgga taggccgtac agcagattct tggcgactgc gcggaaataa
     7561 ctcgcacctt ggaaaaccag gaattcgtcc caaaccttgt tcgaattgat acggccgcgc
     7621 acgcgaaatc cggagaccgg cagggccacc ttgccgagtt gcccggggaa cgtcgattgc
     7681 aggtcgaaca tcgccggcgt cgcaaccaaa tcttccgcga tgccatcttg cacggtcgac
     7741 accgtgaccg ccgtctgcag gttgttggcg gcccgcaata gctccagtcg aaacggcagc
     7801 tgctcggcac gccagatgtc ggcgtcgggg ttgaaacgaa tgctgcgata ttgttccggg
     7861 ctcaacttgt cgagagcggc gggcagtcga ttgcgctgcg gcgcataggg tgtggtggcg
     7921 cgtcgcttgg cgtccgcaag cagcgtatcg aaggtaaagg gatacgtggc cggcggcttt
     7981 tcgggggccg gcgcattggc gccgtgcgct gcaaccgccg tcagggtgag cgccagcacc
     8041 agcaatgtat tcgcgattga aacgggcaag gctccctcgt ttgccggtag ggcgtcggtg
     8101 cgagcccact aaccttgatg aaactgtcga ggtctgtcaa cgtgcattgt atggcggccc
     8161 acgctccttc tggccaggaa ccgcaaagcg cccgcgcggt gctgatggtg cggccggcga
     8221 gctttggctt caatgcagag accgccgcca gcaatgcttt tcagcaggcg ccgcggtccg
     8281 cggatagcgc gacacaaacg ctcgcgctga atgaattcga tggtttggca cgggccctgc
     8341 agaacgccgg tatcgaggtg ttggttgcac ccgacagcga tcaaccgcgc aagcccgacg
     8401 cgattttccc caacaattgg ctgagcttcc atttcgacgg cacggtggtg ctgtatccga
     8461 tgttggctgc caatcggcgc tctgagcggc gcgaggagat tctcggtcag gtggtgcgcg
     8521 agggacggtt tcgcgtcgcg cgcacggtcg atctcacgca tcgcgaggcc gaggggaagt
     8581 ttctcgaggg aaccggcagt gttgtcttgg atcgggcgaa tcgtgtggct tacgccagct
     8641 tgtcagcgcg taccgacttg gatgtactcg gagagtttgc ccagctgctc gactacgaac
     8701 tgatcacctt cgaaacccga gacgtgcacg ccgcgcccgt gtaccacacc aatgtggtga
     8761 tggccatcgg cagcagattc gcggtggtct gcggcgcggc gatcgcgcag ccgctgcacc
     8821 gcgatgctgt tttcaacagg ttgcgggctg ccggccacga gatcgtcgat atcacgctgc
     8881 cgcagatgtg caatttcgcc ggcaatctac tggaactcgc cgccgcgggc ggcccggtca
     8941 tcgcactgtc gacaacggcc tggcgcagtc tcaaaaccgc acagcgacga atgctcgagt
     9001 cgcacgcggc gctggtgccg gcgaatattc ccaccatcga acgaatcggc ggcggcggcg
     9061 tgcgctgcat gctcgcggaa ctgcacctgc cgaaacgcct ataatccgct catgctggaa
     9121 ctcggggtca agttgctcat cgggtacttg ctcggcacgc tgctgggcag tttgatcttg
     9181 ggccgcctgc gcggagtcga tatccgcggc ctgggcagcg gcaatgccgg cgcgaccaat
     9241 gcgttgcgaa ctcagggaaa gctgtttgca ttcctcgtat tgctgatcga cgtcgccaaa
     9301 ggagcgctcg cggtgttgtg gctgccgacg gcgacgctgc cggcggtggg tatcgacccc
     9361 gacgtaccgc gcgagtggtt gacactcggc tgcggcttgg ccgcgatcgt cggccacgtc
     9421 tatcccgtgt ggttcagttt tcgcggcgga aaaggtgccg cgaccgtcgt cggggtgatc
     9481 gcggcggtgg aattgcgtct gctcgtgccg ctgcttgcga gttggttcat cgtgctgctg
     9541 ctgtcgggtt atgtgagcct tgccagcatg ctgtccggcg tcgcgctggt cgtggcggtc
     9601 tgtgtgctcg agccgggcaa tgtgccgctg ttggtgtttt gcacggcgat cgcgctgttc
     9661 atcatctata cccatcgcag caatatttcg cgcttgcgtg cgggtcagga gcatcgcgtg
     9721 caacgactat ggctgtttcg atcgcgagcc gcttagcgca cccgccgctt ctggtgctgc
     9781 tggccgatgg ccggcagcat tccggtgaac ggctcgcgca ggcactcaat gtgacgcgcg
     9841 cgtccgtgtg gaaggcggtg gagcgcctgc gcgccttggg tttggaggtg caagccgcgc
     9901 cgcggcgcgg ctaccgcttg ccgaatccgg tcgagttgtt ggatgtgcgg cgcatcggcg
     9961 cagaatttga tccactgcgg gttgctcggc tgcgcaatct cgaactcctg ttcgacgtgg
    10021 attcgaccaa cagccgattg ctcgccgcgg cggcgccgcc cgcgggctct gcggacgctt
    10081 gcttggcgga atttcagcat gccggtcgtg gccgccgcgg caggcgttgg attgcgccgt
    10141 tcggcggcag cgtggcgcta tcgatggcct ggagcttcgc gggcggagcg cagcttgcgg
    10201 cgctcagcct cgccgccggg gtcgccgtgt ggcgtgcgct ggcgcgcgcc ggtgcgcgcg
    10261 gcgtgactct caagtggccg aacgacatct ggttccaaga ccgcaagatc ggcggcgtgt
    10321 tgaccgagtt gcgtaccgaa tccggcggtc ccgtgcatgc cgtgatcggt gtcggcatca
    10381 acgtcgcctt gggcgcggcg gcacgccgca agatcgaagc gagcggagtg caggtcgcgg
    10441 cgctggcgga cgcctgtgct gccgcgccgt cgcgcaactt gctggccggc gccctgttgg
    10501 atgagctttt gagtatgctg gtcgaattcg agcgagcagg cttcgccgcc ttccgggaga
    10561 attggatgga actcgatgcc ctcggcggcc ggcaagcgaa ggtattgctc ggcccgatgg
    10621 tgatttcggg cacggcgcgc ggtgtcgatg aggacggagc cctgctgctc gagacggccc
    10681 atgggacgca acgattcgtg tccggcgagg ccagtttgcg cgtgcaagag ggtgatcctt
    10741 gacgttgtta aaaggccttg tcctctgcct ggtgctggcg aacgtcggct acttcctgtg
    10801 ggcccgcggt atcgacactg cgccgcaagc gccgtcccaa gcggctcccg gctcgctcaa
    10861 gctcgcctcc gaagccgtcc aaggcaacct cgccagcggc accgccgccg gcggtgcgat
    10921 tgcgggcccc aagggcgttg cggccaacag cagcgcgacc aacaacggcg tcgcggccaa
    10981 tggggctgtc actgccgaca acggcgccac cgcgaacagc gccccctccg cggtgagcgg
    11041 cgcgccggat gcggttgtcg cggacgccgg cgaggcggga aagccggcgc tgctcaacaa
    11101 tgtgcagcgc tgtatcagcg tcggaccgtt ccgcgacgtg gcggaagccg cgcatgcggc
    11161 aacgaccctg cgcggcggcg gctacgatcc acggcagcgc gtcgcggacg gcgatgtctg
    11221 ggcgggagtc tgggtttatt tgccgctgcc ggctgcgcgc gccgccggcg atcagattct
    11281 ggcgaaactc aaggcgggcg gtatcgatga tgcgctggaa atgcccggac cgaccgaggg
    11341 ttcggtcata tccctcggtt tgtacagcga accgaagcgc gcccaggcca gagttgctca
    11401 agcgcaggcg ttggggttca atccgggcgt agcagaccgt aagcgcaccg gcaatgtgta
    11461 ttggatagac atcgacttga aaccgaccga cagtttgttg aatcccgcag atttgcaggg
    11521 cgaatcagga cgcatcgtgc gccttgaagt gcgggcttgt ccgtcggctg ccggggccac
    11581 gccttgaatg cacgcgaggc cagcttgaag gtcttcggcg gcatcggcgc tgtgtttggc
    11641 tatgggtgtc tgctggcatt tctctatctc gtcggactgc aggcctaccg ctggtttcgc
    11701 gacggtgaat ggacgcacat cggggtgaac gaaggcctgc gcgcaggcct cgcgcgctgc
    11761 tgcgtcaagg aaggcgatac gggccggctc gcggatttcg tgcaatggct cgacgcgccg
    11821 gtgaattggc tcggcttaca caaggttttc gaagtgttac cggcctcgtt ggccatattc
    11881 gcggtcagca tcgccggaaa ttgcctgttc atctattgtc gggatcgcct cgacgggcgg
    11941 gaaatccagc cttgaccgta gcggctgtct gcagcgcccg accgtgaatg accccatatt
    12001 gacgctgttg cgactgaaga cgctgctgcg gagcgtgatt ctgccgccca ccgggccgct
    12061 gctgctcgcg gttctaggca tcgttctgat caagcgtcga ccgcgcctgg cgcgagcctg
    12121 cctgatcgct gctatcggca cgctttggct gctgtcgaca ccggtgatct ccgatgcgct
    12181 ggtccggtgg gtcgagcgtt acccgccact ggtcatatcg agtaccggcg gcgctcaggc
    12241 gatcgtgatt ctgggcggcg gcggagagcg ggcatttgcg ccggaatacg gcggcccggc
    12301 tgcggacccg accctgctcg aaaggctgag ctatggcgcg tacctcgcgc gcaacaccgc
    12361 gttgccgata ctggtgaccg gctatcacat cgaggccatg gcgatgcaag cgagcctcga
    12421 gcgcaatttc gatctcacgc cgcgttgggt cgatgcagat gcatacgaca ctttcgagaa
    12481 tgcgaccaac tcagtgcagt tgctgaagac ggccggcatt catcgcatca tcctggtcac
    12541 ccatgccacg cacatgtggc gcgccgtgca agagttcaac gctgccggca tcgaagtcgt
    12601 gccggcaccc gtgggaatca gcgccgagcg cgaacccgga ctcgggcgct acattccaaa
    12661 ccccgaaggg ctgctgcgcg caaatgtcgc gctctacgaa gtgctgggcg agcaagtgcg
    12721 caacgtgctc gccgctgtgc acctgcgccg gcactgaagg gacggtgagc gaggcgatca
    12781 tgcacgcggt gacggcgcga atcgacgctt aagcgactac aatgaccgcc ggtttcgaca
    12841 agcgcgacga ggtacgacat gcgagttcta caaattgtgg gggccatcct gattgccgga
    12901 ggactttttg tcctgatcaa gtcaccgtcc tattcgagcg ataaaagcgt gttcaaaatc
    12961 ggcgatgtcg aagccaaggt ggcacagcag catgcgatct cgccctggat aggcggcgcc
    13021 gccctggccg ccggattggt gctgatcgtg acgggggccc gccgcccgta gctcgcagcg
    13081 gcgacagacg attattttgc caaccgcttg cgcaacgact tgagttcgcg gcgccgcgcg
    13141 gcgctcgtct tgggatagct caatttgagt gccgccaacg tatcgagcac gatgcgggac
    13201 acgatgaggc gggtgttgag cttatcgtcg gcgggtacga tgtaccaggg cgcgcgcttg
    13261 gtgctggtgg cggcgaggca ttgttcgtag gcgcgcatgt agtccttcca gtacttgcgc
    13321 tcctcgatat cggcgagact gaatttccaa ttcttgtcct gcttgtcgat gcgatcgatg
    13381 aatcgcttgc gctgttcgtc tttcgacaga tgcaggaaga attttatgat ccgcgtgccg
    13441 ttgcgctgta gatgtctctc caagtcgacg atcgaacgat agcgctgatc ccagatcgtc
    13501 ttttcattgg tgcttgcgag tccctcgccg cgcaggatct cgggatgcac cttggcgatc
    13561 aagacctctt cgtagtagga acggttgaaa ataccgatgc gcccgcgttc cggcaggtcg
    13621 cgcgtggtgc gccaaagaaa gtcgtgttgc agctcgactg cgctcggatg cttgaagctg
    13681 aacacttggc atccctgcgg gttcacgccg gacatgacat gttgaatcgc cccgtccttg
    13741 ccggccgcgt ccatcgcctg aaagatcagg agcaccgcgt agcggttcga ggcgtacagc
    13801 agctgctgct gttcgctgag accggcgacg tgtttgcgca gcagatctgc gtaatccgag
    13861 ttgctggcgt acgcgggcgc ggcccgcgtc ggccactcgc gcaggtcgac tcgggctcct
    13921 tccggcaccc gaaacaggct cgactttata tgcaccggat ctcccgaggt aggttttatc
    13981 cgacatcaac tgcgaacccc ggcccatggc gatttgcggc cggggcctgc actatgccta
    14041 caaatgagcg gccaaggaac tcggtatgta tagcaggctt gctgcagggg tgctgctgct
    14101 gaccgtgatc ggcctggcaa atgcgcacgc cgcacctttc gaactaccgc cgctcaattc
    14161 acccgcgagt agcgaacatc accccggcaa agtcgtttgg gcggagctgg tgacgcccga
    14221 tctcgctgcc gccgagcact tctatgccag cttgttcggt tggacgtttc agaccgtgca
    14281 tagcggcgca tccgagtatg ccgtggcggc cgtcgacggc cggccggtcg gcggcctgtt
    14341 tcaaaaatcg gtcgcggccg gcgaacacaa acagtcggcg tggttgacat tcatagcggt
    14401 cagcgatgtc gacgccgcga agcgcgcggc cgtggggcag ggcgccaaga tcgtgtccga
    14461 tactcgggac tacccgctgc gcggacggca agccgtgctc gcggatccgg agggagcggt
    14521 gttcgccatt ctcgcctcgc agagcggcga tgcagcggat tttcttgccg cgcccggaga
    14581 atggatctgg agttcgttgc tgtcgcagga tgccgcggct gaagcggcct tctatcaaac
    14641 cttgtttgcc tacgacgtct tcgacctcgc aagcgacgac gactcggagc atctgatttt
    14701 gtcgagcgat ggcttcgccc gcggcagcgt gaattctttc ccggccggcg ccgcgcgccg
    14761 gcacgcgcat tggctcaatt tcgtcagagt cgacaacacc gccgacagcg tcgccaaggc
    14821 cgtggcgctc gggggtcgga ttctggttga gccgcatcct gaccggcacg gaggccagat
    14881 cgcggtgctc gccgatccgg ccggagcacc gttcgggttg atggaatgga cgccgcgcga
    14941 cagcaaggtg gagccgaaat gacgcgcgca cgtcatcgct ggttgacggg cgggctcgca
    15001 gtactggcac tcacgctgct gcatgcgtgc gcagtaaccg gcgtgggcgt cgacggctcg
    15061 gtcggcgtcg gctacgacgc cggctactac gagccatggg gttacgacta cggtggctgg
    15121 ggcggcggct accgcgtcgg cccggggagg ggcggggagc ggcgcggcgg ccatgcgcca
    15181 ccccgctatc ggccggcgcc gagcgggcga cccagtcctt cgattccggg gcgcccacgc
    15241 ggacgctgaa ccggacgcag ttgcaaatcg cgcggcaagg cgcggtggcg ctatagtacg
    15301 gtaaaaaacc agcgcctgat tcttcggagt ccttccttgc ccgatccgac aacgcgtccc
    15361 gacgcgcggc aaacgcgccg catcctgttt gcgagcttga tcggcacgac catcgagttt
    15421 ttcgactttt acatttacgc gaccgcctcg gtgttggtgt tccccaagct gttttttccg
    15481 aagagcgatc cgaataccgc catgctgcag tcgttcgcga cgtttgcgct ggcttttttc
    15541 gcgcggccgg tcgggtcggc gttgttcgga catttcggcg atcgaattgg gcgcaaggcg
    15601 acactggtca cagcgttgct cacgatgggc gtgtcgacgg ttgcaatcgg cttgttgccg
    15661 acctacgcaa ccatcggcgt gtcggcggcg ctgctgctgg cgctttgccg gttggggcag
    15721 ggtttgggat tgggcggcga atggggcggc gcggtgctgc tcgcgaccga aaacgcgccg
    15781 ccgggcaagc acggctggta cgggatgttt ccgcaactcg gcgcgcccct gggatttttg
    15841 tgttctaccg gtgtgttcct gctcatcagc aaccgcttga gcgaggcgca gttattcagc
    15901 tggggttggc gggtaccgtt tttggcgagt gcggtgctgg tgttggtcgg gctgtacatc
    15961 cgtttgcagc tggtggagac gccggcattc aaactcagcc tggcccaagg tgaacgcgtc
    16021 cgggtgccgt ttctggtggt catcgcccgc catccgctgc gcttgctgct cggaacgctc
    16081 ggctcgacct gcaccttcgt ggttttctat ctcatgacgg tattcgccct gggatgggcc
    16141 acgcactcgc ttggttttgc gcgacagcaa ttcctcattt tgcaaatgat cggcgtgttg
    16201 tttttcggct tgacgatccc gctcgccgga atcatcgccg atggcgtcag cggccggcgg
    16261 atgctgatga tcgccacggt tctgatcatc atcttcggtt tcgcgttcca accgttgttc
    16321 tcggcgcaca atacggcgag cgtattgagc tttctggtgc tgggttttgc gttgagcggc
    16381 cttacctacg ggcccctcgg gagcgcgctc gcgacgctgt tcccaacggc cgtgcgctac
    16441 accggcacct cgctcgcctt caatttcgcg ggaatactcg gtgcatcgct ggcaccgtcg
    16501 atcgcgactt cgctggcgga gcgctacggg cttgcctacg tgggatatta tctgtcgtcg
    16561 gccggggccg tgagcctgct ggcgttgctg atgatcaaga acgatgaacc tcgcgttaca
    16621 ggagaatgca aatgatccgc acgacaccgg cagttcggct agcggcgacc ttgttattca
    16681 cggcatgcct gtcgagcggg ctcgaggcga acgccgccga tgcctacgac gccgccgttg
    16741 cgcacgcggg acgcagtgcc gcggatatca aacgcgatca attggatcat ccggccgata
    16801 ttttgcggct gtccggcatc aagcccggaa tgagggtcgc cgacgtgctg gccggggacg
    16861 gttactacag cgagcttgca agttacgtcg tgggtccgac cggtaaggtg ttcctgatca
    16921 acaatacggc gttcgacaag tggagcgaca atctgccggc gcgcctgcag ggcaatcggc
    16981 tgggcaacgt cgagcatcag acgctcgacc tcgaccagat gaatctcaag cccgggagcc
    17041 ttgatgcgat attgctggtc aaggtgtatc acgatttgta ctgggtggat ccggagggcg
    17101 tgtggccgaa ggtcgacgtc ggcggcgtgc tcaatcagtt ggcgcgcgcg ctaaaaccgg
    17161 gcggggtact gctattggtg gatcattcag cgaagccggg gagcggcaat agcgtcgcgg
    17221 gcagcctgca tcggatcgac gagcagttcg cgatcaagga cttcgagtct catggcttca
    17281 aggtggtcgc gacaagcgat ctattgaggc ggccggacga tgcgcgcgac ctcatcacct
    17341 acaaaggtcc ggcggtcggc aagacggatc gattcgttgt ggtgtttcgc aagggcgcgg
    17401 cgtagccaaa gaccgcgcgc ggagcgcggg ctgcgttcac ttcggttgca ccgtcaggat
    17461 ggaaaagctg tcgggaacaa tggcgggccg cgggtgaggc tgcgcatcgg tcgcgggcgg
    17521 cgtggccgcg agtttcgcgg cggcgaggta gatcttatgg gtggcgggat ccagcaccat
    17581 ggtgcgcgcg cttacttgag tcgtgaccgt ggcgaccacc cgatattcgt cgggcgtttc
    17641 ctgatggatc acggtcagcg taccgtccat gccgttcgaa ctgaacacca tactcaaatc
    17701 tggatcgaac gcggcagcgt cggggccgct gccgatcacg acgcgcgcca cggctttgcc
    17761 cgatgtggag tccgtgaccg ccatgatttg gttcgcacac acggagaaca ctcgatgatg
    17821 cgccacatcg agcgcctggc ccgtgggatt tgcacagccc gcgagcggcc aggtggcctt
    17881 gaccgtcatt gatttggcgt cgacggcgac gagtttgccg gggtccgtct cgatgttgac
    17941 gaacacggtg cccgcgtcat cggtcgtcat gaattcaggc ggccccggaa gcggcactgt
    18001 ggcaatcacc tgcagcgtgc cggcatcgac aaccgagaga ttcgacgaat cgccattggc
    18061 ggtgatgatg cggttctggc gcggctcgta caagatggcg tccggctttt tgccggaaac
    18121 gggcacctct tgaatcagcc gcagcgagcc gagttcaaac acggatatgg aatcgctgcg
    18181 gccatcgctg gcgtagccgc gcatcagggc gggcgcaaac gcaatgccgt gaactccggc
    18241 cgtattggcg atcgtcccgg tgattttccc gctgacggtc tcgaccacat cgacccgatc
    18301 tgcgcggctg acgaacaaac gcgcgccgct cgcttcgagc gccaggtagt cccaaccgcc
    18361 ggcgccgccc aacgaccatg tgccggtcac cgtgtacacc ggtgcgggaa gcggcgagga
    18421 atccgttgat gccgtcgccg ctgcgcacca aagcccgagt gcgccgagcg caaacacaag
    18481 attacgcgcc acgttatttt ccttcgattt gaagagcgtc gagtctacct gtattgcgtg
    18541 ccggaaaata tcagacggta atttcgctat gtaatttcta cagcggatgc gatgatcgct
    18601 cgatgcagcg cgattacctg ggcaatccgc ttggcgcgca aagcgcgacg actgcgcgcg
    18661 ccatcgacga tttcatcgaa ggcttgctgg cgtacgagac ccgcgcggag cgtgtgctga
    18721 ttgcggcgga cgaggagccg gcctcgtgca tggccaatgt gtattcaggc ctcttgtgga
    18781 tgttgctcga agcgccggcg ggggccgcga acgcggcaaa atatctggcg gcggccgaac
    18841 gcaccgcggc cgatgcaacg cttcgcgagc agctcaacac ggcgatgttg cgcgcctggg
    18901 tggacgacga tctgctgcag accttgcggc tgtgcgagcg cattgcagat gaattcccgc
    18961 gcgatctcgt catgctcaag atccatcagt acttggaatt caatcgcggc aacccggccg
    19021 cgatgctgcg cgtcgcgctg cgggcgctgc gcgccgcgcc cgatgttgcg tatgtccacg
    19081 gcatggcggc gtttggctat gagcagtgtc atttattgaa cgaggcggaa gcggcagcgc
    19141 gaacggcgct cgacatgcgg cgcaaggaac cgtgggcaca acatgcgctg gcgcatgtgt
    19201 cgctgacgcg cggactgatc gatgagggcg ccgagtttct cgaacaaatg gccgatacgt
    19261 ggagcaactt gaattcgttc atggtgacgc acctgtggtg gcacctggcc ctgttctacc
    19321 tgagtcaggg acgctaccgg cgcgtattgg agctctacga tcgtcactgc tggggcgtcg
    19381 ccaaggatta ctcgcaagat cagatcggcg caatctcgct gctggcaaga ttcgagatcg
    19441 ccggtatcga tgtcgggccg cgctggcggg agctcggcgg ctatctggtc gcgcgcgcgc
    19501 aagatacgac gctgccgttt ctcacgctgc agtacctgta tggattggcg cgcgccgcgc
    19561 ggccggaggc cgacagcttg ttggcggcgg tgcgccgcag ggcggaaact gcgccggcgt
    19621 tcgcgcgccc ggcatggcgt gaagtggcgc tgcccgcttg cgagggactg tatgcccacg
    19681 cgcgcggcaa ctacgacaaa gcttgggctt acttggtcgt gagcgtgccg cgcatggccg
    19741 agatcggcgg cagtcatgcg caacgggatc tgttcgaaca gatcttgttg cacgcggccg
    19801 tcaaagacgg acgtttcagc gccgctcaac aaatgctgga gttgcggcgc ctggccgatc
    19861 cccgcggcgt acccgtcaat accgggctcg ctgccgtgta tgtcgaattg ggcctgccgc
    19921 aactcgcaga gcaggcgcag gcgcgcgcca tgttcacccg caggcagcat ccggcttaga
    19981 cttcaagaga tccccatgcg caacgatccc gtggatggca tattcaagac gatcgagaat
    20041 cggcgcgacg atctcatcga gttgacgtgc gatttgattc gatttccgag cgtcaatccg
    20101 cccggcgagg cgtatcgccc ctgcgcgcaa ttcatcggcg atcgcatgcg cgcccgcggc
    20161 ttcgacgtcg agtatgtgcg cgccgccggc acgcccggtg attgcgacgc ttacccgcgc
    20221 ctcaacgtca tcgcgcgccg gccgggtgcg gcgcccggac cttgcgtaca cttcaatagt
    20281 cacatcgacg tcgtgcagca aggcgccgga tggacggttg acccgttcgc cggcgtggtg
    20341 cagaacggcc gggtgtacgg gcgcggctcc tgcgatatga agggcggtct cgccgcctcg
    20401 atcatagcgc tcgaggcgct gatcgacggc gatgcgaagt tgccgggaac gctcgagatc
    20461 tccggcaccg tcgacgagga gtcgggcgga tacggcggcg tacatttctt ggcggagcga
    20521 ggctggtttt caccgccgcg cgtcgatcac gtgatcatcc cggagccgct gaacgtcgat
    20581 cgcgtatgca tcggccaccg cggcgtgtgg tgggcagaaa ttgaaacgca cgggcgcatg
    20641 gcgcatggct cgatgccgtt tctcggcgac tgcgcggtac gccacatgca cgccgtcgtc
    20701 gagcgtttcg agcgcgagct gtatccgaaa ctcgccgccc ggcgcacggt gatgcccgtg
    20761 gtgccgccgg gtgcgcgtca ttcgacgctc aatatcaatt cgatccacgg cgggctcgcc
    20821 gaagcgccgg gatatccggc ggcgctggta cccgattcct gccgcatggt catcgaccgc
    20881 cgatttctga tcgaggagaa catcgacggc gtcaagggcg aggtggctga gttgctggaa
    20941 cgactggcgg cggagcgcgc cggttttcgc taccggatga gcgacatatt cgaagttcag
    21001 ccgacgatgg cggaccgcga cggtccggtg gcacgagcca ccgccgccgc gatccgccgc
    21061 gtaatgagct gcgagcctga attcatatgt tcaccgggca cctacgatca gaagcatatc
    21121 gatcgggtgg gaaagctcag agattgcatt gcatatgggc cgggaattct tgatttagca
    21181 caccaaccgg acgagtacgt ggtcatcgac gatatgctga acgcggccaa agtgatggcg
    21241 ctggcggcgc gctcgttgct ctaccgaagc gcctgactgt cataacgtgc gccgcaagtc
    21301 gcaacttcct catgccgcta caacgtagtc gacatagcag gtagaataat tgtgggctag
    21361 ttctcaaacc cgcggaatct tgcaatgaaa ttcggaaata gcttcggaaa ttcgagaact
    21421 gcggctcggc ccgagccggc gaaaatgccg tccgcggtgg cgcagcgcag caccccgtgg
    21481 catgcggtct cgattgtcgc caagaacgaa agctgtgacg cggcgcgagc actgcgtaac
    21541 gcccgattcc tgtccgcaca ggcgccgcga ttgccgcttg ctgcatgcac cctgggaggc
    21601 gcatgtccgt gcggctacaa gcaccacagc gatcggcgcg gtcaaccgcg gcgcaaggac
    21661 gaaatcacgg gactcaaaca gaccaacaaa tcacccgtgg agcggcgtgt atcgccggac
    21721 cggcgcgccg ccgattgatc gatcatcgcg gccgatgagt cgcgccgaag gtgcagctga
    21781 aatgcactgc ccgggcgatg gcccgcgcca ggcagtccgc cgccgccgaa ccgatgcgcc
    21841 caatctgaac cgcgcgcggc ggctctgaac gcagcgctaa ctcgccgctg gcgatgctga
    21901 aaatcgtgtc gccgtcgaac ggggtgtgtg ccggacgcag cgcccgcgcg attccatcgt
    21961 gggccatcat cgccacccgt ttgcattcca gcgtggacaa atccgcgttg caggcgacga
    22021 cgccgagcgt ggtgttggcc ccggcctgca agcgaccgag cgacaccagc cgcgaggcgt
    22081 cgggcgcggg gtcgctcgcg tcgatttgcc cggcgggcgc gccggcgccg ccgaattccg
    22141 cgtcgagttc gaagggccat gcccaatacg acttgccgtc gggcatcagc gcggaaccga
    22201 cggagttcac cgccgccagc gcgcccacga tcaaacgctc gcccaaatcc aaggaactcg
    22261 agccgacgcc gccgcccacc accccggcca tggcgccgcg tcccgcaccg tgggcgccca
    22321 gggcaaaatc gccgccggcg gcagcgagcg cgcgaacacc taagccgcga tacggcggct
    22381 cgagtcccca ttgcttgtcg ccaccattgg caagatcgtg cagcaccgcc gcaggcacga
    22441 tgggaattcg cggtgagccc gtgcgcagct gcagaccgat gcctttgacc gagagcgctg
    22501 cggcgacccc gtcggcggcg gccagtccaa acaccgaacc tccggcaagc acgatcgcgt
    22561 gcgcgcggcc gatcagattc tcaggcgcga gcgcatcggt ttcgcgcccg ccgggggctc
    22621 cgccgcgtac gtcgaccgcg gccgaccacc cgtcgccgca aagcagcgcc gtcacaccgc
    22681 tcctgacatg ctcgtcggtg gcgtggccaa cctcgagtcc cgaaatatcg gtaatcaaat
    22741 tcctgggagc aggccgaaac acgcgttgcc tagcgtggct gcgccgcgat gaccgcaaga
    22801 ccttgcagaa ccagatccgg cacgatctcg ccctgcgagc gcatcgtgcc ggtgatcatg
    22861 ccggaatcgc cgcccgtgat gaacaatttc ggcttggtgc cggtgctttg ctcgatcact
    22921 tcggccgccc gatcggccat gcccgccaaa gcgaacacgg cgccgcgcga cacggcgccg
    22981 agcgtgttat tggccagcac gccggcgagc gagggcgtgc tgtgcagtgc cgccttggcg
    23041 atatcgctgg tcttgttcat caaggcctcg cgcatcatgt ggatgcccgg cacgatcagg
    23101 ccgcccaagt gttggccgtt ggcgtcgacg ccgtcgacct tgaccgccgt cccggcatcc
    23161 accacgcaca gcgccgaacg cgctttggtc caggcgccga tcaaggcgag ccaacggtcg
    23221 gcacccaaca acgagggatc gagatagccg ttggtgaggc cattgaattg cgcggacgcg
    23281 gttacgaact cgaccttgac gcgaaatttc ttctttgcga gattgctcaa ggtttttgcc
    23341 atgaccgggc ccgcgacatt cgacaccaga acgcgctgcg gcgtttctcc ggactcgaac
    23401 aaggctgcgg tccacatgcc gattttggca tcgcgatgca cgacggcccg ctgatccgag
    23461 agtccggtac tcgtcagcca agcccactta aggcgggtgt ttcccacgtc aatgaccaga
    23521 atcatgcgaa atgtgttacc caagcgcgcc ggcaatcgcg taagcgcgca aggatagcat
    23581 cgtcgggatc agcgaagtga ggcgaccacg gcaacgaggg attgggaaac gcgctcgtcg
    23641 atctccgctg cattttgcaa gtgagccgga tagcgtacgt gggcgtcggc gccacccgca
    23701 gagaaactca actgcaccct gggttgggaa tcgccgccga tattcgaaga ggtcgcgcgc
    23761 tcgatctcct tggactgctg cacaatctcg gcgcgctcat ctttgacggc ctcggtgacc
    23821 gccgcggtga gcttggcctt ggcggccgcg tagtcgatgc ctgccggaag gctcagggtg
    23881 atctcccgcc acacgaaatt gacgccgtga atctgcttga acaggccgct cgatacttga
    23941 aacacgatcg aattggcgaa cgcgaccact cgacccgtgg gtcccagtag tccgtggcca
    24001 ccaagttcca tgagatgcag cctgaccaac ccgagttcga tgacttcacc ggtgacttcg
    24061 ccgatttgca cgcgatcacc gacgcgaatg ccgtattttc cgatgagaaa gaaatagccg
    24121 acgatggaca cgagcacgct ttgcatggcg acggcgagac cggcggtgat gaggccggcg
    24181 aaggtgacga tcgagcccaa ttcgctggca aacgcgaagc cgatgatcac gaccacgagc
    24241 gcccagaatg cgatcttgcg caacaggaga aactggtagc ggcgccgcgc gtcttgaatg
    24301 tagcgtatga cggcgagccg ccaaacctcc gcggcggcaa acaccagcgc caacaagatg
    24361 aggagtatgc cgacgcgtac ccccaaggtc ttgagcgcgc cgcggtattg gttcctgatg
    24421 gcgtcgcgcc aactggcgag attgcgccgg tattgattga gcagtacgcc ttccttgctc
    24481 aaggggatga gcagcgccga gatctgcttg aactcgctcg acacgaggtc gagctgatcg
    24541 cgcgcgtgat tgagcgccgc gctatccgtg gtgtcgcctt gagcagccac cgcatcgccg
    24601 cgcgccgaca aggtttttag atcatcgatg atgggtccgc gaatccgcgc gaaggtgttt
    24661 tgcaaagccg cggcccgtgc atcgatctca tccacggtgg ccgctttctc cgagagccgg
    24721 atcacattgg cggccaaatc ccagatgccg aatcgagctg cggcgattcc gctgatgctg
    24781 ttgagcgggg tcgacgcagc ggccggcgtc tgttgcgccg ggcccgctgc agccgtgagg
    24841 gctgcgccgc cgccggaagc agcagcattg gccggcgcgg gagtggcgga caaggtcacg
    24901 gccatggcgt cgatctgcga tcttaagccg ctcgcgctga aaccaccggc atcgctttga
    24961 ctggtcaggc tcgacatggt cgcgagcagg gttgttctcg cgtcgatcaa ctcgagttcg
    25021 ccctgcagtt ccgagatttt tgcctgcagt acgtttcttt gttcggctgc cgacttggcg
    25081 agctgcgttt gcaccgattc gagttcgctg tgcacggcgg cgccttgcgc ggcgtacttg
    25141 gattcgagct ggctcagctg cgtggccgca gcggtgccgc ccgaatcctt ggttgcgggt
    25201 tgcttggcga ggacatcgac gtcggcgcgc gcgatctcga acgccaaggc gatgacttgg
    25261 tttgctatct ggcgattgtc atacaagatc aacaagtcgc tcggctcgtt ggcggcttgc
    25321 tgctgaatgc cgagcgtgcg aaaccagtcg atcgcttgat ccagcacttg aatgacccgc
    25381 tgcccggtca agggcgcggc gattgcggcc ggcgccgcgg ccgggggggt ggccggggcg
    25441 gctgcggcca atgcctggaa cgatacgagc agggcgggcg ccaatccggc cagtacggga
    25501 taaaggttta gcgtgcgcat agtcggaacg ctaccatatg gcagcgcggc gcggactacg
    25561 gcgtcgagcg ggatcgagtt gcgacttttc aatctgtgcg gtgatcatgt ttgacttcaa
    25621 gcaggcgatt tccgacgtgg ccgacttccc gcgccccggt attttattcc gcgacatctc
    25681 gcccttgttg cgcgatcacc tcgaagtcac ggtgcaagcc ttggacgggt tgctcacgga
    25741 ggcggaatgg agcgggatcg acgcgctggc ggggatcgaa tcgcgcggct tcattttggg
    25801 cgcggcgctg gcactgcgcc gcggtaaggg attcgtgctg gtacgcaagc aaggcaagct
    25861 gccgccgccg gttgtcgacg tgacgtacga gctcgagtac ggatccggcg tgctggaaat
    25921 gcagcgcggg caagggcggg tgatcctggt ggacgatgtg ctggcaaccg gcggtacgat
    25981 gagcgccgcg gcggatttgt gcgttcgggc gggctaccgg ctgcacgcgt tgattgccct
    26041 gatcgatttg cacatcgtcg ccaactactc ctggcgcagt ctgcctttgc gggcagtcat
    26101 caattactaa cggtggcgca tgcgaccttc aggcgctggt gaaatcggcg ctgctcggct
    26161 gtttgctgac ggctttttgc gcgagtgcct gcgccggtgt gcgagtgatc gtcgggccga
    26221 cgccgatcaa gggcggcgag gcgaagtcgg cgggcgacat cacggtggtc aacgagaaac
    26281 tggccttcgc gctcgccgtg caatcgccgg ttccctacgg tgtgccgcgc ggcgcgatca
    26341 tcgacgtcgc accggtggtg aacggggaaa tcggccgcga ttgcgtggtg ttcgcggact
    26401 tcattccgaa taactggtcc gcgtggccca acacctttca acacatcgaa attctgcagc
    26461 gcggccccac gcgggtcgtg gtgcgcacgg tgcgcgactg gggcaaggtg acgatcacga
    26521 cgctgtacac gctcgcagcc gcggccgact cgattgcgat acgcaccacc atgcacaacg
    26581 gcggcgccgc gttggccgat ctattgtcgg gtctgacgct gtggccgaaa ggcggccatt
    26641 ttttcggcgt tccgggattg agaggagtgg tcgaggggag cacggagatg gcgctcgccg
    26701 atcgcgtggt tgcctatgac gagcaatgga gcgttgcgct gcatgcgccg tatgccgatc
    26761 acatcgccga tgggtcgctc gatttgtacc ggctgcacac gctcgccgcg ggggagagcc
    26821 gcgtgttcga aggctggctg caagtcggcg cgagcgggga tttgaagccg gtgatcgcgg
    26881 ctgaaattgc ccgcaggcat ctgacagccg gaaccgttcg cggtaccgtc gccgcgagca
    26941 acgcgcagcc ggtcgaagcg ccggtgatcg tcgtcgaaaa acacggcaag ccgtacggct
    27001 gggtgctggg acgcggcggc gtgttcgagt tcgcgcttgc gcagggcgat taccttctgt
    27061 acgccaccgc caagggctac tccgagagtg cgcgaatccc gctgcaagtc aaacccggtg
    27121 cggtggccaa cgtggatttc cgcgagctgc ggccgccggg aaatattcgc tggtcggtgg
    27181 cggatgcgcg cagcggcgcg ccagtcgacg ccaggatcaa catcgaggaa ggtcagaaac
    27241 cgctggtgga gtttctcggg cgcaacacgt ttttcaccga actcgagcgt cgcggccgcg
    27301 tcgatgtgcc tattgcaccc ggtgattacc gtttctcggt gtcctcgggc ggaggattct
    27361 tggcccaatc tgagcgcgtc aaactctcgg ttgcgccggg agaggcgcgc gccgcacagg
    27421 tggcgctgac ccggctgttc gacccgccgg cgcggcattg gtacgcggcg gatctgcatc
    27481 accacgccga tcaggcggaa gcggtgacgc cgccggcgga cttggcgcgc tcgcaactcg
    27541 cggcaggact cgatttgctg ttcgtcagcg atcacgattc aaccgtgaat cacgcgacgt
    27601 tacgcgagct tgccgaacgc cgtggcgtcg ccttcattcc gggtatcgag ctgtcggcct
    27661 cttggggaca cttcaatgca tatcccctgg cgcccgatca gacattggcg atcgacacgg
    27721 ggaccgcaag catcgatgaa ctcttgactg cggcccggcg catgggcgcg acggtggtgc
    27781 aggtgaatca tccgttcatc ccttacggct atttcaccag cgtctcgcgg ggcgtggcac
    27841 ccggcggttt caatccgagc ttcgatctca tcgagatcaa cgcggccgca cccgccgacg
    27901 atgcgaaggt tttgcgcacg ctgtggaatt tctggaatgc cggacatcgc tactacctgg
    27961 ccgccggcac cgacacgcac gatgtgtggc acgaggagtc gggagcggtg cggacgtttg
    28021 cgcatatcga cggcgcggtc accgcggttg cctatgcaca ggcgctgaag gacggacatg
    28081 gctacgttac gttcggcccg ctgatcttcc cctcggtcat attcggcgac gagttgcggg
    28141 tcaagccgcg cgaaccattc aatttgggct tcgatctgca ggcggttgcc ggcctcaaac
    28201 aggtcgagtt gatcgagtcc gggagggtct tgaagactga ggcctggacg gcggcatcgc
    28261 agcaggtgca cgtcgatttc ccactgacta ccgagcacgc gacgtggtat tcgctcatcg
    28321 tcgaagacaa ccaggggcac aaagcctata gcgacccgat ctgggtcgat accgttgcgg
    28381 gactgacccc ggagtcccgc gccgcgcaat aacggcgact gtctctcgtt ttaaggcatc
    28441 tcctaaaaaa aactacaaaa acagcctttt tctgcgaagg acctcacagg gcgcggaacc
    28501 gcaatatgta acgttgcgga ttatgttgac gagcatcatt cagcgagcgg cccttgggct
    28561 cgtagtcctt gccggaactc cggccctcac ctacgccaac agctgcgccc cggcgacggg
    28621 gcagggtacg gccccgagcg attatcagga ttactgctgg ttggatttca ccggctactc
    28681 ggacgccatg gcccgggcgg gaggccagcc cttcaccttc accctgcccg acgggtcgac
    28741 gctctcgatg acggtgcagg tcagcaccaa caagaccaat cccgccttgg tggcgcacat
    28801 cgtaccctcg tggaccggtt cggccatcgg caacaaagcc ttcaccggga tccccggcca
    28861 acccgtgctg tacgagaccg tgaacggatc caccgttcag ctcgcgctct cgaatatcac
    28921 ggtagtgccg ccgccggggg gcggagcgag tgcgtcctac gccatgatcg cggcggacgg
    28981 cgagtccacc aaccagaccg agcagctggt tttcaccacc aatggtcagg cgtggaccca
    29041 ggtggcgcag atcgcccaag gcaccttgta cccaacggtg tcgggtgtgg gaacgaacac
    29101 ggtgaccgag accggcgttg ccggaacggt cggcagcttc gtgttcgcct ccttcaacaa
    29161 tcccacgcaa ctgtcggcga ctctggtcgg cggcggactg cagggcgcga tgttcgccat
    29221 tcgctatgcc tcgctcgcgg cgaacgcgca attcagcggc gctcgcgcca gcgccagcga
    29281 tcagttcacc tacggcatct ccaccaccgg cggtcagtcg attgcctcgg ggacgacctc
    29341 gggcagtggc acgggaccct tcaccccggc cgtggcaccg acgatcgcgg cgtcctatcc
    29401 cttcgtcgtg agcgagacgc tcgcggcggg cagtaccagc accctggcga actatgcggt
    29461 ctcgctcacc tgcaccaacg gagcgagcgg aacctcgtcg acggttctgc cgaccaatct
    29521 tgcaaatcgc acttatacgt tctcctcgct ccagtacggc gatgccgtgt cgtgcgtgtt
    29581 caccaatacc gcgaaccgcg caaatttgac ggtcgcgaag agcgggccga cctcggtaag
    29641 tgcaggcgca ccggtgagct acaacgtggt ggtcgccaat gccgggccgt ccgacgccgg
    29701 cggcgcattc gtgaaggatc cggcggtcgc gaattttacg gccctagcgg tgacctgcgg
    29761 gtcagccgcc ggcggtgcgg tctgtcccac cgcgggcctc accatcgcaa atctgcaagg
    29821 ctccgggatc ctgatcccga cgctgccgag cggtgggtcg gtcacattcg ccgtcagcgg
    29881 caccgccggc acgggcacca tcgtcaatct tgcctcggtc acgccgccct cgacggtggt
    29941 gaattccaac accacgccga cctcgagcgc gacaaccacc gtgaccccgc caccggacgc
    30001 cctcagcacg gccgcttttc cagtgaacgt ggccgccggg cagccggtga acggcacggt
    30061 gacgttctcg aacgggggct cgggaatcgc gaacaacacg acgttcagca tgacgttggc
    30121 cgcgaatctg gcggctgcga ctctcacggg attgccggcc ggcgccacct acgcctacgc
    30181 gccggggacg ggcatcgtcg cgttcgccgg catgccgacg accttggcgg ccggcgcttc
    30241 gatcggaccg atcggcgtgc actacacgca gccgagcgcg ggcacttcga cggtgaccgc
    30301 gacggtgtca accaccgcgg atagcaaccc cgccaataac accgtcagcg tcgtggtcgg
    30361 tgggccgccg gacgccatca gcagcgtggc atttcctgcc agcgtctatc ccggtcaacc
    30421 ggtcagcggc acggtgagct tctcgaacgg cggcaccggg attgcgacga atacgagttt
    30481 tgggatgacg ctgccggcca atctagctgt tgcgcccacc ttgacgggct tgccggcggg
    30541 cgcggcctac agttatgccg cgggcacggg cgtgaccacg ctcaccggca tgccgacgag
    30601 cattgccgtg ggcgcctcgc tcggaccgat cggcgtgaac tatctgcaac ccgcgagcgg
    30661 cagctccacg gtgaccgcgg ttgtcgcgac caccggcgac agcaacccga gcaataacac
    30721 cgcgacggcc gtgatcggcg gcgggccgcc ggatgcctcg agcgccgcgc aattcccggc
    30781 cacggtcaat ccgggtcagc cggtgaatgg cacggtcacc ttctcgaacg gcgggttggg
    30841 ggtcgcaacc aacaccacct tcgcaatgac gctgccggcc aatctcgcga cggcgccgac
    30901 gctgaccggc ttgccggcgg gtgccggtta tgcttatgca cccggaaccg gcgtcatcac
    30961 gttcaccggc atgccgacga cgctcgccgt gggcatcccg gtgggtccga tcggcgtgca
    31021 ctacgtgcag cccgcgtccg gaacctccac cgtcaccgcg gccgtcaccg acacgggtga
    31081 cagcaacccg agcaataaca gcgtcacggt ggtggtcggt ggcggcgcac ccgatgcgca
    31141 aagcgtggtc gctttcccga acgcggtgga tccgggtcag ccggtgaacg gcacggtcac
    31201 cttctcgaac ggcgggttgg gggtcgcaac caacgccacc ttcgcaatga cgctgccggc
    31261 caatctcgcg acggcaccga cgcttgccgg cttgccggcg ggcgttacct acgcctacgc
    31321 gcccgggagc ggcgtgatca ccttgaccgg catgccgacg agcttggcgg cgggcagttc
    31381 catcggcccc atcggcgtgg gttatctgca gcccgcgagc ggcagctcga ccgtcatcgc
    31441 ggtcgtcacc accaccggcg acagcaaccc gagcaacaac acagcgaccg cggtgatcgg
    31501 cggcgggccg ccggatgcca tcgccacggc cgcgtttccc gccaacgtca ttgccggcca
    31561 gccggtcaac ggcaccgtga cgttttccaa cgcggggctt gggcccgcga gcaacgccag
    31621 ctatgggatg acgctgccag ccaaccttgc gacgccaccc actctcaccg gcttgccggc
    31681 gggtgccagt tacgcgtatg cgccgagcag cggggc
//
LOCUS       DASWZT010000113         5082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_58885, whole genome shotgun sequence.
ACCESSION   DASWZT010000113 DASWZT010000000
VERSION     DASWZT010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58885"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VGE92_03010"
     CDS             complement(<1..481)
                     /locus_tag="VGE92_03010"
                     /inference="COORDINATES: protein motif:HMM:NF025098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312815.1"
                     /translation="MNSYPRWKIALVAVVLLIGILLAVPNLFGEESALQLARDRAAVL
                     DVDRTAVEQILKEKGVTPSGAFLDQGRLTLRFSSKADQLKARDLINEARPNQFTIALS
                     EASRVPEWMRNLGLSPLKLGLDLRGGVDLVYQVDVQGAVKQLLDRHEQDFRASLRSAH
                     "
     gene            complement(478..810)
                     /gene="yajC"
                     /locus_tag="VGE92_03015"
     CDS             complement(478..810)
                     /gene="yajC"
                     /locus_tag="VGE92_03015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEY1312816.1"
                     /translation="MDFFINVAKAQAAGASGGQGGLVSMLMFVPILAIFYFLIIRPQN
                     KRAKEQREMISKIVAGDEVATTGGILGKVAEVGDQFLTLEIASGVHVKLQKFQVAQVL
                     PKGTVKSA"
     gene            complement(883..1998)
                     /gene="tgt"
                     /locus_tag="VGE92_03020"
     CDS             complement(883..1998)
                     /gene="tgt"
                     /locus_tag="VGE92_03020"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004697695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEY1312817.1"
                     /translation="MQFELLARDGAARRGRLKFPRGSVETPAFMPVGTYGTVKAMTPE
                     DLEAIGAQIVLGNTFHLFLRPGLEVIAAHQGLHRFMHWRRPILTDSGGFQVFSLAQMR
                     KITEEGAHFRSPVDGAQVFLSPEESMRIQRVLGSDVAMSFDECTPYTASESEARASME
                     LSMRWAARGFRAYYRTESPGTLFGIVQGGIHPQLRLASLAALEELGFAGLGVGGLAVG
                     ESQAERELVLDGLVPHMPLRKPRYLMGVGRPLDIIEAVRRGIDMFDCVMPTRHARNAH
                     LFTRGGVVNIRNAVHQRDTGPIEEACACYTCRNYSRSYLRHLDKCGEILGAQLNTIHN
                     LYFYQRLMREIRAAIESGRFESYAAEFYALQAAADAR"
     gene            complement(2016..3062)
                     /gene="queA"
                     /locus_tag="VGE92_03025"
     CDS             complement(2016..3062)
                     /gene="queA"
                     /locus_tag="VGE92_03025"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEY1312818.1"
                     /translation="MQRTDFHFDLPRDLIAQWPTARRSDSRLMVLDGACGSRRDLQFR
                     DFPSLLSRADLLVFNDTRVIPARVFGVKESGGRVEILLEHAVTANTALVHLRASKGLK
                     SNAVVKLPGGHSASMLGREGELFRLQFSCEVATFFETHGEIPLPPYIERQAQSADRER
                     YQTVYARSRGAIAAPTAGLHFDADTFAELAARQIHHVFVTLHVGAGTFAPVRVDDVES
                     HEMHEEYMTVPAAACDAINATRAAGGRVIAVGTTVVRSLETAAHASPRGIAPYQGSTR
                     IFIKPGHRFRVVDAMLTNFHLPESTLLMLCSAFVGREPLLAAYSHAVRAKYRFFSYGD
                     AMFLTPSAAAREPA"
     gene            3118..3204
                     /locus_tag="VGE92_03030"
     tRNA            3118..3204
                     /locus_tag="VGE92_03030"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3152..3154,aa:Leu,seq:caa)
     gene            3228..3770
                     /locus_tag="VGE92_03035"
     CDS             3228..3770
                     /locus_tag="VGE92_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY1312819.1"
                     /translation="MNFCSNCGSPVEFKVPAGDHLPRFVCSSCGVVHYKNPLLVLGCV
                     PEWQNKILLCRRAIEPRLGFWTVPAGFMENGETLQAAAARECYEEALVKVEIGSLLAV
                     ASVTGASQVHVMFRAKLLQPEFAPGPESLEVALYDEAEIPWTELAFPSGEFTLRKFFE
                     DRAAGREDHHFVELQRRLKS"
     gene            3838..4161
                     /gene="fdxA"
                     /locus_tag="VGE92_03040"
     CDS             3838..4161
                     /gene="fdxA"
                     /locus_tag="VGE92_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016138692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEY1312820.1"
                     /translation="MAFVVTESCIKCKYMDCVEVCPVDCFHEGPNFLVIDPDECIDCT
                     LCEPECPVEAIFSEEEIPDGQESFLKLNAELAKKWPVISEKKDAPADAKQWEGVAEKL
                     KLLER"
     gene            complement(4169..4912)
                     /locus_tag="VGE92_03045"
     CDS             complement(4169..4912)
                     /locus_tag="VGE92_03045"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEY1312821.1"
                     /translation="MLKLVATLALSLGAAGLGARALAADPAIDPRVALLKRLPTGSKV
                     EDLRPSPIPGVYEFTQGADVSYLTADGKYFLDGTLYDMDTHANLTEELRARARLAMIS
                     AVPESQMVIFGPKNPQYTITVFTDVDCAYCRKLHGDMAELNRLGVRVRYMFYPRTGPN
                     TESWKKAEVVWCSANRNEALTRAKAGATLDLTKTCAATPVAREYELGQSIGVRGTPAI
                     LTEHGDYLAGYSPPRELVQQLNDLRLAKR"
BASE COUNT          988 a         1574 c         1561 g          959 t
ORIGIN      
        1 cgtgcgcgct gcgcagcgac gcgcgaaaat cctgctcgtg gcgatcgagg agctgcttga
       61 cggcgccctg cacgtcgacc tggtacacga gatcgacgcc gccgcgaaga tccagtccca
      121 atttcagcgg gctcaggccc aggttgcgca tccactccgg aacgcgcgat gcctcggaca
      181 acgcgatggt gaattgattg ggacgcgcct cgttgatcag atcgcgggcc ttcagctgat
      241 ccgccttgct gctgaagcgc agcgtcaggc gtccctgatc gagaaacgcg ccgctcggcg
      301 tgacgccttt ttccttgagg atttgctcga ccgccgtgcg atcgacatcg agcacggcgg
      361 cgcggtcgcg cgcgagctgc agcgcgctct cctcgccgaa cagattgggc accgccagaa
      421 gaatgccgat cagtagcacg accgcgacca acgcgatctt ccagcgcggg tacgaattca
      481 tgcgctcttg accgtgccct ttggcagcac ctgggcgact tggaatttct gcagcttgac
      541 gtggacgccg ctggcaattt ccagcgtgag gaactgatcg cccacttcgg caaccttgcc
      601 gagaatgccg ccggtggtcg ccacttcatc gccggcgacg atcttggaaa tcatctcgcg
      661 ctgctctttg gcgcgtttgt tctgcggcct gatgatgagg aagtaaaata tggccaggat
      721 cggtacgaac atcaacatgc tgaccaatcc gccctgcccg ccactggcgc cggctgcctg
      781 cgctttggct acatttatga agaaatccat agggttacac tcgtttttgg cggcggacgc
      841 cacgacacaa agcggcgtag tatacagcag acgtgcacaa acttaacgcg cgtccgcggc
      901 ggcctgcaaa gcatagaact cggccgcata gctctcgaac cggccgctct cgatcgccgc
      961 gcgaatctcg cgcatcaggc gttgatagaa atacaggtta tggatggtat tgagttgcgc
     1021 ccccaatatt tcgccgcact tgtccaaatg gcgtaaatag ctgcggctgt agttgcggca
     1081 ggtatagcaa gcgcaagcct cctcaatcgg cccggtatcc cgttgatgca cggcgttgcg
     1141 gatattcacc actccgccgc gcgtgaacag atgggcgttg cgcgcatgtc gcgtcggcat
     1201 cacgcagtcg aacatgtcga tgccgcggcg caccgcctcg atgatgtcca gcgggcgtcc
     1261 gacccccatc agatagcgcg gtttgcgcag cggcatgtgc ggcaccaggc catcgagcac
     1321 cagttcgcgc tcggcttgac tctcgccgac cgccagcccg cccacgccaa gccccgcgaa
     1381 tccgagctcc tcgagcgcgg ccagcgaggc tagtcgcagc tgcggatgaa taccgccctg
     1441 aacgatgccg aagagcgtgc ctggagactc cgtccggtag tacgcccgaa agccgcgcgc
     1501 ggcccagcgc atcgacaatt ccatggacgc gcgggcttcg ctttcgcttg ccgtataggg
     1561 cgtgcattca tcgaaactca tggccacatc cgagcccagg acccgctgaa tgcgcatcga
     1621 ttcctcgggt gacaggaaca cctgtgcacc gtccaccggc gagcggaaat gtgcaccctc
     1681 ttcggtgatt ttgcgcatct gcgccaagct gaacacttga aagccgcccg aatcagtgag
     1741 gatcggccgc cgccaatgca tgaagcgatg caacccctga tgcgccgcga tgacctccag
     1801 gcccggacgc agaaacagat gaaacgtgtt gccgagcacg atttgcgcgc cgattgcttc
     1861 aagatcctcg ggggtcatgg ctttgaccgt gccgtaggta ccgaccggca tgaacgccgg
     1921 tgtctcgacg ctgccgcgcg ggaacttgag gcggccgcgc cgcgccgcgc catcccgtgc
     1981 cagcaactcg aattgcatcg gcccaagagc ggcgctcatg caggctcccg cgccgccgcc
     2041 gagggtgtca ggaacatggc gtcaccgtag ctgaaaaatc ggtattttgc gcgcaccgca
     2101 tggctgtagg ccgcgagcag cggttcgcgt ccgacgaagg ccgagcacag catcaacaac
     2161 gtcgactccg gcaaatgaaa attcgtcagc atcgcgtcca ccacgcggaa tcggtggcct
     2221 ggcttgatga atatgcgtgt cgatccctgg tacggcgcga tgccgcgagg gctggcgtga
     2281 gccgccgttt ccagactcct gacgaccgtc gtgcccaccg caatcacccg gccgcccgcc
     2341 gcgcgcgtcg cgttgatcgc gtcgcaggcg gccgcgggca ccgtcatgta ttcctcgtgc
     2401 atctcgtgag attcgacgtc atcgacccgc accggtgcaa acgtaccggc gccaacgtgc
     2461 agcgtgacga agacgtggtg aatctgccgt gctgcaagct cggcaaaggt gtcggcatcg
     2521 aaatgcagcc ccgcagtcgg cgccgcgatt gcgccgcggc tgcgcgcata cacggtttgg
     2581 tagcgttcgc ggtcggcgct ttgcgcttgg cgctcgatgt agggcggcag cgggatctcg
     2641 ccgtgcgtct cgaagaatgt cgcaacttcg cacgagaact gcaaacgaaa caactcgcct
     2701 tcgcgtccca gcatgctggc gctgtgcccg cccggaagct tcaccacggc attcgacttc
     2761 aatcccttgc tggcacgcag atgcacgagg gcggtgttcg ccgtcacggc gtgctcgagc
     2821 agaatttcga cgcggccgcc gctctccttg acgccgaaaa cccgcgccgg tatcacgcgc
     2881 gtatcgttga aaaccaataa atcggcgcgg ctcaacagcg acggaaaatc gcggaattgc
     2941 agatcccgcc gcgatccgca ggcgccgtcc agcaccatca gccggctatc ggaacgccgt
     3001 gccgtgggcc attgggcaat gagatcccgg gggagatcga agtgaaagtc cgtacgttgc
     3061 atttaagttt tgcattctac caagagcccc gatcgcatat actgcccggc ctggcatgcc
     3121 cggatggcgg aactggtaga cgcgccggac tcaaaatccg gtggtggtga caccgtgtgg
     3181 gttcgattcc ctctccgggc accagaattt tattttatgg acctcaattg aatttctgca
     3241 gcaactgcgg ctctcccgtt gaattcaaag tccccgccgg ggaccatttg ccgcgcttcg
     3301 tttgttcgag ctgcggcgtg gtgcattaca agaaccctct gctggtctta ggatgcgtgc
     3361 ctgagtggca gaataaaatc ttgctctgcc gccgcgccat cgaaccgcgg ctcggatttt
     3421 ggacggtgcc ggccggcttc atggaaaacg gtgaaacctt gcaggcggcg gccgcgcgcg
     3481 aatgctacga agaggcactg gtgaaggtcg agatcgggtc gctgctggcg gtcgcgagcg
     3541 tcaccggcgc gagccaggtg cacgtgatgt tccgcgcgaa gttattgcag cctgaatttg
     3601 cgcccgggcc cgagagtctg gaggtcgcct tgtacgatga ggccgaaatt ccgtggacgg
     3661 agctcgcgtt tccgagcggc gagttcacgc tgcgcaagtt ctttgaggat cgcgcggccg
     3721 gccgcgaaga tcaccatttc gtcgaattgc agcgccggct gaaatcctga ccctgccgcc
     3781 aaaagcggcg cggtcgggta gtattcatat ccagtaggtc aagaaaaggg taagtcaatg
     3841 gcattcgtgg tcaccgaaag ctgcatcaaa tgcaaatata tggattgcgt ggaagtctgc
     3901 ccggtcgatt gcttccatga aggcccgaat tttctggtga tcgatccgga tgaatgcatc
     3961 gactgcacgc tgtgcgagcc ggagtgtccg gtcgaggcta ttttctcgga ggaagagatt
     4021 cccgacggac aggaaagctt cctcaagctc aacgccgagc tggcaaagaa gtggccggtg
     4081 atcagcgaga agaaagatgc ccccgccgat gccaagcaat gggagggggt cgccgaaaaa
     4141 ctcaagttgc tcgaacgcta gcgccgaact agcgcttggc aagccgcaag tcgttgagct
     4201 gttgtaccag ctcgcgaggc ggggaataac ccgcaagata gtcgccgtgc tccgtgagaa
     4261 tcgccggggt gccgcgcaca ccgatgcttt ggcctaattc atattcgcgg gccaccggcg
     4321 tcgcggcaca agtcttggtc aaatccaacg tggccccggc tttggcgcgg gtcagcgctt
     4381 cgttgcggtt cgccgagcac cacaccactt cggctttctt ccagctctcg gtatttgggc
     4441 cggtgcgcgg ataaaacatg taacgcaccc gcacgccgag ccggttcagt tcggccatgt
     4501 cgccgtgcaa cttgcggcaa tacgcgcaat cgacatcggt gaacaccgtg atcgtgtatt
     4561 gcgggttctt cggcccgaag atcaccatct gcgattccgg aaccgcactg atcatggcga
     4621 ggcgcgcgcg cgcgcgcagt tcctcggtca gattcgcatg cgtgtccata tcatacagag
     4681 tgccgtccag gaaatacttg ccgtccgccg tcaagtaact tacatccgcg ccctgcgtga
     4741 attcgtagac gccgggaatg ggcgacggcc gcaggtcttc caccttcgat ccggtaggca
     4801 ggcgcttcag cagcgccacg cgcgggtcga tggccggatc ggcggcaagc gcgcgcgctc
     4861 cgagtccggc agcgcctaaa ctcaaggcaa gtgtggcgac aagtttcagc attcgcgggg
     4921 actccggggg aacttgacta tccgaccctg cggaaacgcg aaggttcagg cgattctaac
     4981 agggccgcct gccgaatact ctagccgcgc ggatggtgct gcgaatgcag ttccttaagc
     5041 cgttcgcggg cgacgtgcgt gtagatctgc gtggtcgaca gg
//
LOCUS       DASWZT010000114         8175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_5620, whole genome shotgun sequence.
ACCESSION   DASWZT010000114 DASWZT010000000
VERSION     DASWZT010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8175
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5620"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VGE92_03050"
     CDS             complement(<1..765)
                     /locus_tag="VGE92_03050"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY1312822.1"
                     /translation="MPTPSELRPNPDLLLAKVTRQEAEARRGKLRIFFGSSAGVGKTY
                     AMLVAARKLKAEGRDVVIGIVETHGRSETAALLGDLEVLPPRPMSYRGKDLSEFDLDG
                     ALLRHPSLILLDELAHSNVQGARHPKRWQDAEELLAAGIDVFTTLNVQHLESLNDVVG
                     GITNVRVWETVPDTVFDDADEVVLVDIPAEELLERLKAGKVYLPQQAERAANNFFRKG
                     NLMALRELALRRTADRVEGDVQAYRVDKSIGSVWKTA"
     gene            complement(884..1900)
                     /gene="ltaE"
                     /locus_tag="VGE92_03055"
     CDS             complement(884..1900)
                     /gene="ltaE"
                     /locus_tag="VGE92_03055"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: protein motif:HMM:NF007825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HEY1312823.1"
                     /translation="MTQYNIDLRSDTVTRPTTGMRAAMAAAEVGDDVYGDDPTVNRLQ
                     ATMAERFGFENALFFASGTQSNLAALMSHCGRGDEYLVGQEAHTYSYEGGGAAVLGSI
                     QPQPLQNEPDGAILIERIASAIKPRDIHFAPTRVLALENTIGGRVLSQAYQTEVTQFA
                     HSRGLITHLDGARVFNAIVKLNIVERDAAAGFDSVSVCLSKGLGAPAGSVLLGADDFI
                     AQARRWRKVLGGGMRQAGVLAAAGLYALEHHVALLEQDHDNATFLAHGLRALGLNVEP
                     PQTNILYVEIPVEHTARLKNHLAQRGILATVGPRTRLVTHLDLPRAKIEAVLQAFRAY
                     RDWG"
     gene            2042..5545
                     /locus_tag="VGE92_03060"
     CDS             2042..5545
                     /locus_tag="VGE92_03060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHYT domain-containing protein"
                     /protein_id="HEY1312824.1"
                     /translation="MQATYNPSLVALSVIVAVLVSYTALSLAARVAAANSLQVRFWLI
                     GGAVTMGVGIWSMHFIGMMAFTLPIALRYNIATTLLSLTVAVMTSGGALWIAGGRLLG
                     WRRLGLGAVLMGMGISAMHYSGMSAIQIVPMISYDPLLVAVSIGIAIVASFAALWLAF
                     NLRSGDSIRMIIGRLAAALIMGAAISGMHYTGMAASRFSRGAYCFGGLPIDNQWLAMI
                     IGLITVALLAITLITTIFDAHLQSQVAAQAQRLKEVNSELQLQAAKAQASEERLRQIA
                     DNIPAMVAYWDRDGICRFANKTHLERLGLTPEQLVGMSLDEVFGHGNIADHQVDAGLR
                     ARIDAALKGERQLFDQTDVDADGTVRHSQSEFLPHWVGDQVMGFYALIVDITQRKIAE
                     SRLAQQEARLTTTSRMGEIGGWELERGAPGPLWSDMVYRIHDLPVGEMPKLESALNFY
                     PPGARETIAAALTAAFEEAKSYDLVLPFITATGRHRWVRTIGEPQMRDGKCVRIAGAF
                     QDVTESRQAEETLRIAKEAAEAANRAKSDFLANMSHEIRTPLNGVIGMTGLLLETTLG
                     AQQREYAEIVRSSGESLLALINDILDFSKIEAGRLELESLDFDIQNVLEDTIDAIALR
                     AAEKGLELIVDIEPDTPRIFRGDPLRLRQVLLNLLSNAVKFTGCGEVSLALSSAGAGD
                     KTRLIFEVRDTGIGIPADRINTLFAPFIQADSSTTRRFGGTGLGLSISKRLAEAMGGS
                     IEVDSTVGQGSMFRFAVCLQQSEASLASKVDNQLVGLRVLIVVGHRGSRRTLDRQLTP
                     EGCNLTFAATADEGMAQYHAMLRADRPPVAIIIDHDLDDRSGLEFAAAIRASAAPPAS
                     LILLSSMSTSLSESDLRLVDRVVTKPAKSAVLVRALAELTRASGPTVPATDAAPGALP
                     FSGTRILLAEDNPVNQKLATRLLQRLGVEVQVAANGIEALQALRDADFDAVVMDCQMP
                     LMDGYEATQRLRSADGAVRNPNVPVIALTAHALATDRAKCMQAGMNDYLTKPINPMHL
                     QQALSKVLPGAERRAANTGAAVLFDESALLGRTDQDRKFARELINMFISSTSDTITQL
                     SACVHGGADPDLVRKLAHGLKGSAATAAATAVSACAADLERVAGSAQAVAALNSLDAA
                     FLQTAAEWQRLGWTTSKDAVA"
     gene            complement(5526..>8175)
                     /locus_tag="VGE92_03065"
     CDS             complement(5526..>8175)
                     /locus_tag="VGE92_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEY1312825.1"
                     /translation="AAPGVKAAAQKLSEPDFEDSHWYAAVVPGTVLTTLIARGAYPDP
                     DYGLNNLAIPDSLSRQDYWYRSTFYVPADLHDQDLTLIFKGINYAAEVWLNGERLGDI
                     RGAFVRGVFNVTGKVHPGSTNALAVRVSPPPHPGIPHEQSIAAGPGNNGGNLAIDGPT
                     FIAAEGWDWIPGIRDRNTGIWQEVDLKATGKLRLLDPHVITRLPLPRIDSADVSLNVA
                     VENRAASKVAATLVASFDAIQVRKEVILEPGVTQVLFEPREFPQLHLLSPRLWWPNGY
                     GAANLYHLNLEVLAAGARSDATTLNFGVRELTYELSLFDAAGRLRRVEVDPSEGSALG
                     QRLVDVRHEAIKRTPQGWAASLTPAGENSRAVRPIATESLTPYLAIRVNGVPIAARGG
                     SWGMDDALKRISRSRLEPYFRLHRDAHLNIIRNWLGQNTEEVFYDLADEYGLLVLNDF
                     WVSTQDFQVEPQDPALFLANARDVISRYRNHPSIAVWFGRNEGVPQPILNEGLADLTA
                     SLDGTRYYTGSSNSVNLQGSGPYNYRPPEEYFTDLALGFSVEVGTPSLATLESLRASI
                     PEPDRWPLSDTYAYHDWHFGGNGDVATFMSALAQQYGAGTSLEDFERKAQLLDYVSYR
                     AIFEGFQAHLWSRNSGRLLWMTHPSWPSNTWQIYSSDYDTAAAYYAVKKACEPLHAQL
                     NLPDFTLTVANISRQPQSHLRLVTRIVSLDNRLLAERVDKLGVAANSIATLPPLDLQP
                     LLARARVALVKLTLQDSMGTTLSDNLYWQAWDAASQRLLSDLRPQTISMAAQARNVEA
                     ASRVEVVLKNQDRAPALAAKITMLDAAGERVLPVYYDDNYVSLLPGESRRIEIECPAA
                     SRRCERVALRGWNIAPVEIRVRPPRP"
BASE COUNT         1561 a         2572 c         2519 g         1523 t
ORIGIN      
        1 ggcggttttc caaaccgagc cgatggactt gtcgacccgg taggcctgta cgtcaccttc
       61 cacccgatcc gccgtacggc gcagcgcgag ttcgcgcagc gccatgagat tgcccttgcg
      121 gaagaaatta ttcgcggcgc gttccgcctg ctgcggcaga tacaccttgc cggccttcag
      181 ccgttccaga agttcttccg ccggaatgtc gaccagcacg acctcgtcgg cgtcgtcgaa
      241 caccgtatcc ggcaccgtct cccagacacg aacattggtg atgccgccga cgacgtcgtt
      301 gaggctctcc aagtgttgga cattcagcgt ggtgaataca tcgatccccg ccgccagaag
      361 ttcctcggcg tcctgccaac gcttgggatg acgcgcgccc tgaacattcg agtgcgcgag
      421 ctcatcgagc aagatcaagg aaggatggcg cagcagcgcg ccgtcaagat cgaattcgct
      481 gaggtccttg ccgcggtacg acattggcct cggcggtagg acctccaagt caccgagcag
      541 cgccgccgtc tcggagcggc cgtgagtttc cacgatgccg atgacgacgt cgcgcccctc
      601 ggccttgagt ttacgggctg ccaccagcat ggcataggtt ttgcccacgc cggccgagga
      661 accgaagaag atccgcagtt taccgcgccg cgcttcggct tcttgacgcg taaccttggc
      721 aagcagcaaa tcgggattcg ggcgtaattc gcttggggtc ggcatcgcac cgctattgtg
      781 cgcgatcgag ggccatattg agcaataaaa cgttcactcg cggttcgccg aaaataccca
      841 ttgccggccc tctatgtggg ccaggacgag ttcgcgcagc gccctagccc cagtcgcgat
      901 aagcgcgaaa tgcttgcagg acagcctcta tcttggcgcg cggcaaatcc agatgcgtca
      961 ccagccgcgt tcgcggaccg acggttgcca ggataccgcg ctgcgcgaga tggttcttca
     1021 ggcgcgccgt atgctccacc ggaatctcga catagagaat gttggtttgc gggggctcca
     1081 cgttcaatcc aagtgcgcgc aacccgtgtg ctagaaaagt ggcgttgtcg tgatcttgct
     1141 cgaggagtgc cacgtggtgc tccaaggcat acagcccggc cgcggccaac acacccgcct
     1201 gacgcatacc gccgccgaga accttgcgcc agcggcgcgc ttgcgcaatg aaatcgtcgg
     1261 cacccaacaa tactgacccg gcgggtgcac cgagtccctt cgacaggcag acggatactg
     1321 aatcgaagcc cgcggcggcg tcgcgctcga cgatgttgag cttgacgatg gcgttgaaga
     1381 ctcgcgcgcc gtccaaatga gtgatcagcc cccgtgagtg ggcaaactgc gtgacctctg
     1441 tctggtatgc ctgcgacagg acccggccgc caatcgtatt ctcgagcgcc aaaacccggg
     1501 tcggcgcgaa atggatgtcg cgaggcttaa tggcactggc aatcctctcg atgagaattg
     1561 cgccgtccgg ttcattttgc agcggttgcg gctgaatgct gccgagcacc gcggctccgc
     1621 caccctcgta actgtaggta tgtgcctcct gaccgaccag gtattcatcg ccgcggccgc
     1681 aatgcgacat caacgcagcc aaattgcttt gcgtgccgct cgcgaaaaaa agcgcattct
     1741 cgaagccaaa gcgctcggcc atcgtcgctt gcaggcggtt cacggtaggg tcgtcgccgt
     1801 agacgtcgtc gcccacttcg gcggccgcca tcgccgctct catgcccgtc gtcggacggg
     1861 tcaccgtgtc actgcgcaaa tcgatgttgt actgcgtcac atccaacttt gtgccctcca
     1921 aataatcccc gccggcgcgc aattatcgcg aaaaaaaagc ggcaaaaccc aagctttcgg
     1981 ctcaattctg ccccgtttcg gccgataaga caccgcgggg tgcccgcgtc gcaacgccac
     2041 aatgcaagcc acatacaatc cctccctggt tgcgctatcc gtgatcgtcg cggtgttggt
     2101 gtcgtataca gcgctgtcgc tcgccgcacg cgtggcggct gccaactccc tacaggtgcg
     2161 attctggctg atcggcggcg ccgtcaccat gggcgtcggt atttggtcga tgcattttat
     2221 cggcatgatg gccttcacgc tgccgatcgc tctgcgctac aacatcgcga caacgctgct
     2281 ctccttgaca gtcgccgtca tgacctcggg cggtgcgttg tggatcgcgg gcggccggct
     2341 cttgggatgg cgacgcctgg ggctgggcgc cgtcctcatg ggcatgggta tcagcgccat
     2401 gcactactcc gggatgagcg caattcaaat tgtgccgatg atcagctacg atccgctgct
     2461 cgtcgcagtc tccatcggca tcgcaatcgt cgcctcgttt gccgctctgt ggctcgcctt
     2521 caatttacgt agcggcgatt cgatccgcat gatcatcggc cggctggctg ctgcgctcat
     2581 catgggcgcc gcgatcagcg gcatgcacta taccggcatg gccgcttctc gattcagccg
     2641 cggcgcctat tgctttggcg gcctgccgat cgacaaccag tggctggcga tgatcatcgg
     2701 cctgatcacc gtcgcgctgc tcgccatcac tctcatcacc acgattttcg atgcccattt
     2761 gcaatcgcag gttgccgcac aggcgcaacg cctgaaagaa gtaaacagcg aactgcaatt
     2821 gcaagcggcc aaggcgcaag cgagcgaaga gcgtctacgg caaattgccg acaacattcc
     2881 ggcgatggtc gcgtactggg accgggatgg catttgccgc ttcgccaaca agactcattt
     2941 ggagcgcctc gggctcacgc cggagcagct ggtcggcatg tcgctcgacg aagtgttcgg
     3001 ccacgggaac attgccgatc atcaggtgga tgccggcctg cgcgcacgaa tcgatgccgc
     3061 cctcaagggc gagcgccagc tattcgatca aaccgacgtc gatgccgacg gcaccgtgcg
     3121 gcattcgcaa agcgagtttc tgccgcactg ggtcggcgac caggtcatgg gattctatgc
     3181 cctgatcgtt gatatcacgc agcggaaaat cgccgaaagt cgcttggcgc agcaggaggc
     3241 tcgcctgacg acgaccagcc gcatgggaga aatcggcggc tgggaactgg agcgtggcgc
     3301 acctggcccg ctttggtccg acatggtcta tcggatccac gatctgccgg tcggggaaat
     3361 gccgaagttg gaatcggcgt tgaatttcta tccgccggga gcgcgcgaaa cgattgcggc
     3421 cgccctcacg gcggcatttg aggaggccaa gtcgtacgat ctggtgctgc cgttcatcac
     3481 tgccaccggg cggcatcgct gggtacgtac gatcggcgag ccgcagatgc gcgacggtaa
     3541 gtgcgtgcgg atcgccggtg cgtttcaaga cgtcaccgag agtcgccagg ccgaggaaac
     3601 tttgcgtatc gccaaggaag cggccgaggc cgcgaatcgc gccaagagcg acttcctggc
     3661 caatatgagc cacgagatcc gcaccccgct caacggtgtc atcggaatga ctggtctgct
     3721 gctcgagacc acgctcggcg ctcagcagcg cgaatatgcc gagatcgtgc gctcgagcgg
     3781 tgagtccctg ctggctttga tcaacgacat cctcgacttt tctaaaatcg aggccggtcg
     3841 cctggaactc gagtcgctcg acttcgatat tcagaatgtc ctcgaggata ccatcgacgc
     3901 gatcgcgttg cgcgcggcgg agaagggctt ggagctgatc gtggatatcg agcctgacac
     3961 accgcgtatt ttccgcggcg acccgctgcg attgcgccag gtgctgttga atctactgag
     4021 caacgccgtc aagttcaccg gttgcgggga ggtgagcctg gctttgagca gcgccggcgc
     4081 cggcgacaag acaagactca tattcgaagt gcgcgacacc ggcatcggca tacccgccga
     4141 tagaatcaac accctgttcg cgcccttcat tcaagcggac agctcgacga cgcggcgctt
     4201 tggcggcacc ggtctcgggc tttccatatc caagcgcctt gcggaagcga tgggcggcag
     4261 tatcgaggtc gacagcaccg tcgggcaagg ctcgatgttt cgttttgccg tgtgcctgca
     4321 gcagagcgag gcctctctcg ccagcaaagt ggacaatcag ctggtaggac tgcgagtcct
     4381 gatcgtcgtc ggccatcgcg gcagcaggcg aactctcgac cgtcaactga ccccggaggg
     4441 ctgcaatctc accttcgccg cgacggccga cgaaggcatg gcgcagtacc acgccatgct
     4501 cagagccgat cgcccgcccg tcgccatcat aatcgaccac gacctggacg atcgcagcgg
     4561 gctcgagttt gcagcagcga tacgcgcctc cgccgcaccc ccggcatcgt tgattcttct
     4621 gagttccatg tcgacatcgc tttccgagtc cgatctacgc ttggtcgacc gggtggtgac
     4681 caagcccgcg aagtccgcgg tactggtccg tgcgctggcc gaactcaccc gggcaagcgg
     4741 gcccactgta ccggcaaccg acgcggcgcc cggcgcgttg ccgttctccg gaacgcgcat
     4801 cctgctggcg gaggacaatc cagtgaatca aaaactcgcc acgcggctgc tgcaacggct
     4861 cggcgtggag gtgcaagtcg ctgccaacgg cattgaggcg ctgcaggcgc ttcgcgatgc
     4921 agattttgat gccgtggtca tggattgcca aatgccgcta atggatggtt atgaagccac
     4981 gcagcgcttg cgcagtgcgg acggagcggt caggaatcca aacgttccgg tgatcgcatt
     5041 gaccgcgcac gccttggcga cagaccgcgc caaatgcatg caggcgggca tgaacgatta
     5101 cctgacgaaa ccgatcaatc cgatgcactt gcagcaggcg ctgagcaagg tgctgccggg
     5161 cgcggaaagg cgcgcggcaa atacgggcgc agccgtgctg ttcgacgaat cggcgctgct
     5221 tgggcgaacc gaccaagacc gaaagtttgc gcgcgaattg atcaatatgt tcatcagctc
     5281 gaccagtgac acgatcacgc agctcagcgc ctgcgtgcac ggcggcgcgg accccgactt
     5341 ggtacgcaag ctcgcgcatg gtctcaaagg ctcggccgcg accgctgcag cgactgccgt
     5401 gtcggcctgt gctgccgatc tcgagcgggt ggccggcagc gcgcaggcag tggcggcgtt
     5461 aaattcgctc gatgccgcct tcttgcagac cgcggccgag tggcagcggc tcggctggac
     5521 gacatctaag gacgcggtgg cctgacgcgg atttcaacag gcgcgatgtt ccagccgcgc
     5581 agtgcgacgc gctcgcagcg cctggaagct gccggacact cgatctcgat gcggcgcgac
     5641 tcgccgggca gcagcgagac gtaattgtca tcgtaataga ccggcagcac gcgctcaccc
     5701 gccgcatcga gcatggtgat cttcgccgca agcgccggtg cacggtcctg attcttcagt
     5761 accacctcga cgcggcttgc agcttctacg ttgcgcgctt gcgcggccat gctgatcgtc
     5821 tgcgggcgca ggtcgctcag cagtcgctgg ctcgccgcat cccaagcctg ccaataaaga
     5881 ttgtccgaca gcgttgtgcc catggaatcc tgcaaagtca gtttcacgag cgccacgcgc
     5941 gcccgtgcca acagcggctg aagatcgagc ggcggcagcg tggcgatgct gttcgcggct
     6001 acgccgagct tatcgacgcg ctcggcaagg agccgattgt cgaggcttac gatgcgcgta
     6061 acgagacgca ggtgagattg cggctggcgc gaaatatttg cgaccgtgag tgtgaaatcc
     6121 ggcagattga gttgcgcatg cagcggctcg caggccttct tgacggcgta gtaggcggcc
     6181 gccgtatcgt agtcggaact gtaaatctgc caggtgttgc tcggccacga gggatgcgtc
     6241 atccacagca gccgcccgct gttgcggctc cacagatggg cctgaaagcc ctcgaagatc
     6301 gcgcggtacg agacataatc cagcaactgc gccttgcgct cgaagtcctc gaggctggtg
     6361 ccggcgccgt actgctgcgc gagcgcggac atgaacgtcg ccacatcgcc gttgccgccg
     6421 aaatgccaat cgtgatacgc gtaggtgtcg gacaaaggcc aacgatcggg ctccggtatc
     6481 gacgcccgca gcgactcgag cgtcgctagc gacggcgtgc ccacctcaac cgagaagccg
     6541 agcgctagat cggtgaaata ctcctccggc ggtcgatagt tgtaaggccc gctaccctgt
     6601 aagttcacgg agttcgaact gccggtgtag taacgggtac cgtcgaggga cgccgtcaag
     6661 tccgccagcc cctcattgag gatgggctgc ggcactcctt cgttgcgccc gaaccaaact
     6721 gcaatcgacg gatggttgcg gtagcggctg atcacatcgc gcgcgttggc aagaaacaac
     6781 gccggatcct gcggctcgac ttgaaaatcc tgggtcgaaa cccagaagtc gttcagcacc
     6841 aataggccgt attcgtccgc gagatcgtag aacacctcct cggtattttg tccgagccaa
     6901 ttgcgaatga tgttcagatg cgcgtcgcga tgcagacgaa agtagggctc gagtcgcgac
     6961 ctcgaaattc gcttgagtgc atcgtccatt ccccaactgc cgccgcgtgc cgcaatcggc
     7021 acgccgttga cgcggatggc gagatacggc gtcaacgatt cggtggcaat cggccgcacg
     7081 gcgcgcgaat tctcgcccgc cggcgtcagt gatgcggccc aaccctgcgg cgtgcgcttg
     7141 atcgcttcgt gtcgtacgtc caccaagcgc tggcccaagg cgctaccttc gctcggatcg
     7201 acttcgacgc gccgcagccg gcccgcggcg tcgaacagcg acaattcgta cgtcaattcg
     7261 cgcacgccga aattcagtgt cgttgcatcg gaacgggcac cggcggcaag cacttcgagg
     7321 ttcaaatgat ataggtttgc cgctccgtag ccgttcggcc accacagccg cggcgacaaa
     7381 agatgcagct gcggaaactc ccggggctcg aacaagacct gagtgacccc gggttcgagg
     7441 atgacttctt tgcgcacctg gatggcatcg aagctcgcga ccagcgtggc tgcgaccttg
     7501 gatgcagcgc gattctcgac ggccacgttg agcgatacat cggcgctgtc gatacgcggc
     7561 aacggcaagc gcgtgatgac atgcggatcc agcaagcgca acttgccggt cgccttaaga
     7621 tcgacctcct gccaaatgcc ggtgttgcgg tcgcgaattc ccggaatcca atcccagccc
     7681 tcggcggcta tgaaggttgg gccgtcgatc gccagattgc cgccgttgtt tccgggcccg
     7741 gcggcgatcg actgttcgtg gggaatgccg gggtgaggcg gcggcgatac gcggaccgca
     7801 agcgcattgg tgctgcccgg gtgcaccttg ccggtgacat tgaacacgcc gcggacgaac
     7861 gcaccccgga tgtcgcccaa ccgttcaccg ttcaaccaaa cctccgcggc gtagttgatt
     7921 cccttgaaga tgagcgtgag atcctgatca tgcaaatcgg caggcacata gaatgtgctt
     7981 cgataccagt agtcctggcg gctgagcgag tcggggatcg ccagattgtt caggccatag
     8041 tccggatccg ggtacgcgcc gcgcgcgatc aaggtggtca acaccgtgcc gggaaccacg
     8101 gccgcgtacc aatgcgaatc ctcgaaatcc ggctccgaga gtttctgcgc cgcggccttg
     8161 actcccggcg ccgcg
//
LOCUS       DASWZT010000115        14753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_63767, whole genome shotgun sequence.
ACCESSION   DASWZT010000115 DASWZT010000000
VERSION     DASWZT010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14753
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63767"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VGE92_03070"
     CDS             complement(<1..878)
                     /locus_tag="VGE92_03070"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY1312826.1"
                     /translation="MRAFAWFLACILMAGVIGAALAYPAYELSSSFAPWAFHRVASRI
                     AMLILLAELIWLCRHLRLTRKRDFGFGLPWRRFLRVCVLWGLIGIATAGVGAAFLLGT
                     ELRVRQAQFVLSAANFLHIFLVGLGSGISVALIEETVFRGAMHSAIERESGPWTAALL
                     SAPLFAVLHFFAKASIPVAELGWGSGFDLLLRSFAPLSHPALVFDSFVSWLVVGLILS
                     LTRVMTGNIAVAIGLHAGWVIVLRILQQSTAAGSSPRFSAWVGHFDGLLGFWLLPWGL
                     VIAAALWLGRPAWVFYA"
     gene            complement(882..2153)
                     /gene="hemL"
                     /locus_tag="VGE92_03075"
     CDS             complement(882..2153)
                     /gene="hemL"
                     /locus_tag="VGE92_03075"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005157947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEY1312827.1"
                     /translation="MSLFQEACKYIPGGVNSPVRAFRGVGGEPIFFKKAQGAYLTAAD
                     GRRFIDYVGSWGPMILGHAHPQVIAAVQAVAADGLSFGAPTELETMLARRVVELMPSV
                     ELVRFVSSGTEATMSAIRLARGFTGRDTIVKFEGCYHGHSDSLLIRAGSGALTLGVPT
                     SPGVPAALANYTVTLNFNDAEQVREVFRQIGGQIACVIVEPVAGNMNCVPPAPDFLDT
                     LRRECDSSGALLLFDEVMTGFRVALGGAQALYGVRPDLTTLGKIIGGGMPVGAFGGRR
                     DVMEHLAPMGPVYQAGTLSGNPVAMMAGLTTLELLSAPQFHERLSAATGELTRRLAES
                     ARDAGIAFATNQVGGMFGLFFTAEANVDRYSKVMACDVERFKRFFNGMLAEGVYFAPS
                     AFEAGFLSAAHTPADIDATIVAAKRVFARIR"
     gene            complement(2163..3581)
                     /locus_tag="VGE92_03080"
     CDS             complement(2163..3581)
                     /locus_tag="VGE92_03080"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1312828.1"
                     /translation="MHSLFLRIFVLFWIAMALIVGGSIAITFTIAAHEYEAPELQRRP
                     AVAIQASDALARGGIVGLKHWLDANKNSIADRDLFIVAPDGTDILGRRLSQGAARRME
                     FFNRDEFANRDENARPPRAFGEPPPPGNFRPSRAAPQIVAPDGAAYTVVLVPRRPSIF
                     GALSLPGISATILCIALVVSALISWWLAQHLSAPIRRIQAGARTLASENLEQQNAGVR
                     VSAGLEGRKDELAVLARDFDAMADQLRANRSASTQLLRDISHELRSPLARMRVAVGLA
                     RQPPADTSRQLDRLEREIERLDALISQVLKLARLTGTDTPFARETFDLDEVIEEVVRD
                     ANFEGAVKNCRVRLQGAADAAVNGNRDLLRSAIENVLRNAVRYSPPDGAVEVVVTGGE
                     SVVILVRDRGPGVPEADLTRIFEPFYRVAESRDRDTGGEGIGLAITAQVMKAHGGSAS
                     ACNRQGGGFEVRLSLPPGTVAV"
     gene            complement(3574..4284)
                     /locus_tag="VGE92_03085"
     CDS             complement(3574..4284)
                     /locus_tag="VGE92_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY1312829.1"
                     /translation="MDPTPREARVLLVDDDRELCQMLTEYLVTEHFDVKSVHDGGDAL
                     DALRVEHFEILILDVMLPTVGGLDVLRKLGAAYPTPILMLTARGDDVDRIVGLELGAD
                     DYLSKPFNPRELVARIRAILRRANNQGKSAVADELVVGPITLHTGTHQVHVGGAAVAL
                     TGAEFRVLEILMRSAGQVISRESMTEQALGRKLVAYDRSIDTHISNLRRKLDLEAGKN
                     PEIKNVRGSGYTLTWAHA"
     gene            4440..4967
                     /locus_tag="VGE92_03090"
     CDS             4440..4967
                     /locus_tag="VGE92_03090"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEY1312830.1"
                     /translation="MKSIRNVLKATLLTAGAVLTAATSLSLATAADEAATTTPPPPGP
                     QGWHHHGPMHLLSQLGLSATQKQQVKDIMTAARPEMQSLHEQIHANSLKLRQTQPTDP
                     NYSSIEAQVSQTHGSLSAQMMTQQAEVRAKLFKILTPAQQTQLAALEAEMQAKHGARG
                     GPRGPGPDVGAPPSE"
     gene            complement(4996..5853)
                     /locus_tag="VGE92_03095"
     CDS             complement(4996..5853)
                     /locus_tag="VGE92_03095"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEY1312831.1"
                     /translation="MRLALCGLLLCVTARADTALHSLWEVRGKHNTVYILGSIHVLRL
                     SDYPLAPALLEAYSNAKALLMEVNLADSGSAEVQAEMLASATLPEGKTLPAVLGKERY
                     GRADALAHEVGIELSTFDQFAPWFAAEAISQLQLTQLGFQPEAGVEMYFIQRAKTDGK
                     SVAGLETIHDQISLFENMSLDTQAEYLVSSLEQAHDLPKEVDAMVQAWQRGDTHWFAT
                     HLESDLKQDSGLYQSILGARNRKWVPKIEALLNDDKNYLVIVGAGHLAGSGSVIDLLK
                     KDGIGATQR"
     gene            complement(5871..6638)
                     /locus_tag="VGE92_03100"
     CDS             complement(5871..6638)
                     /locus_tag="VGE92_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY1312832.1"
                     /translation="MQILQITDPHLYGSATGRLRGVETDSSLHAVLDAAFARVPDFSA
                     VLVTGDLVQDDSSGYLRFRSIFGNLKKPVLCIPGNHDEPEAMRRELAGAPFQICGSHE
                     ADGWQFIMLDSYDPGHVGGRLTPAELERLDATLAASPKHAMVCLHHHPITMGSRWLDT
                     VGLAAPEDFWRVIDAHEHVRAVVWGHVHQAYDGQRGPVRLYATPSTGAQFLPKSDRYE
                     LDSRPPAYRHFDLHADGRIDTAVHWLESLPMYQSVAR"
     gene            6844..8844
                     /gene="tkt"
                     /locus_tag="VGE92_03105"
     CDS             6844..8844
                     /gene="tkt"
                     /locus_tag="VGE92_03105"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004812554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY1312833.1"
                     /translation="MPTRRELANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWRDV
                     LKFNPGNPSWWDRDRFVLSNGHASMLLYSVLYLTGYPLSIDDIKNFRQFSSKTPGHPE
                     REVHLGIETTTGPLGQGFANGVGMALAERALAATFNRPGHNIVDHHTYVFLGDGCMME
                     GISHEVASFAGVQRLGKLILVYDDNGISIDGKVVGWFGDHTAERFEAYGWHVQPRVDG
                     HNSDAVAAALAAARAETGRPSLICAQTIIGWGAPNKQGTASMHGEAMGNEEIAATRLN
                     LKWSSPPFEIPADIRAAWDMRDKGARVEQEWRTRFAAYASEFPALAAELERRIEGNLP
                     SGFADLVSAYIGRTQVEGKALATRQSSQAALNAIGPSMPELLGGSADLTPSNGTLRKD
                     SVALTPDSPGGNYVHFGVREFGMSAILNGIVCHGGIIPYAGTFLTFSDYARNAVRMAC
                     LMRLRVVFVYTHDSIGLGEDGPTHQPIEHLASLRDIPHMNLWRPCDAVETATSWGVAL
                     EHRDGPTCLIFTRQAVVHEAREAAQVANIRRGGYVLIDSAGPPEAIVIATGSEVGIAA
                     EAVRTLTAKGRKIRLVSMPSTNTFDSQDEAYKQSVLPSAVTRRVAVEAGVRATWWQYV
                     GLQGEIVGIDHFGASAPAKELFKAYGFTPQHVIEAIEKVLAK"
     gene            8868..9881
                     /gene="gap"
                     /locus_tag="VGE92_03110"
     CDS             8868..9881
                     /gene="gap"
                     /locus_tag="VGE92_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY1312834.1"
                     /translation="MAIKVAINGYGRIGRNILRALYEGKHAGEIKIVAINDLGDANTN
                     VHLTRFDTVHGRFRGDVHVDGDSMVVNGDRIRVVAQRDPSKLPWGELGADFVLECTGL
                     FTSKAKASAHIAAGAKKVIISAPGGDDVDATIVYGVNDGVLKASHSVISNASCTTNCL
                     APLVKVLHEKVGVLSGVMNTIHSYTNDQVLTDVYHSDLRRARSATMSMIPTKTGAAAA
                     VGLVLPELKGKIDGFAIRVPTINVSLVDLSFVAARKTSIEEIHGAVKAAAAGALKGIL
                     AYNDAPLVSTDFNHDPASSTYDATLTKVIEGNLVKVCSWYDNEWGFSNRMLDTTVAWS
                     KAT"
     gene            9891..11066
                     /locus_tag="VGE92_03115"
     CDS             9891..11066
                     /locus_tag="VGE92_03115"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004973295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY1312835.1"
                     /translation="MKLIKMASLDLRDKRVLIREDLNVPIKDGAVSSDARIRAALPTI
                     QAALTARARVLVMSHLGRPEEGKYAAEFSLAPVAKRLAELLGVPVKLQQNWLDGVDVH
                     PGEVVLLENVRFNTGEKKDSEELARKMAALCDVYVMDAFGTAHRAEASTHGVARYAPV
                     ACAGPLLVSELSALETALEKPARPLLAIVAGSKVSTKLTVLESLLGKVDQLIVGGGIT
                     NTFLAALGFNVGKSLYEPAMLDICKRLLEQSSKRGIVIPMPTDVVVAKEFSAKAEADV
                     KPVGAVSPEEMILDIGPDSAEAMSELVASAGTILWNGPVGVFEFEQFGEGTRTLATAI
                     ARSKAFSLAGGGDTLAAIEKYGIEDSISYISTGGGAFLEFVEGKKLPAVEILEQRAA"
     gene            11063..12499
                     /gene="pyk"
                     /locus_tag="VGE92_03120"
     CDS             11063..12499
                     /gene="pyk"
                     /locus_tag="VGE92_03120"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY1312836.1"
                     /translation="MSALRRTKIVATLGPATDDPAVLADIVRAGVDVVRLNFSHGAVA
                     DHARRVELVREAARKAGRYVGVLGDLQGPKIRIDRFTTGKVQLADGAEFTLDAALAVD
                     AGTEHSVGIAYKKLPTDVFPDDVLLLNDGQISLQVLSIDGPRIKTRVLVGGELGNSKG
                     INRQGGGLSAGALTDKDRADIRTAASLKVDYLGVSFPRDASDMDEARKLLRDAGGHGL
                     LVAKIERAEAIKNLAEVVRASDGAMVARGDLGVEMGYAELAGLQKQIIQEARHQNRVV
                     IMATQMMESMITNTIPTRAEVSDVANAVMDGTDAVMLSAETASGKHPAKVVEAMSQII
                     VGAEKYQVAHVRIRQRSSGYFGHTDEAIAAAVMYTANHLHVKAIVALTESGSTSLWMS
                     RVRSDIPIYAFTRHEATRCRVTLYRGVYPVAYDIPEKGEAVYFSIFRLLLDLNLVAEG
                     DSIILTKGELSGVSGGTNSMQILQVTRK"
     gene            12500..13351
                     /locus_tag="VGE92_03125"
     CDS             12500..13351
                     /locus_tag="VGE92_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004879284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY1312837.1"
                     /translation="MPQEFRGTDRYIATEELMAAVNAAMVLSRPLLIKGEPGTGKTLL
                     ANEIARSLNKPFFEWHIKSTTKAQQGLYEYDAVSRLRDSQLGDPRVKDIHNYIVKGDL
                     WEAFDCEVQPVLLIDEIDKADIEFPNDLLRELDRMEFYVYETHETVRAKHRPVMVITS
                     NNEKELPDAFLRRCFFHYIRFPDAATMTQIVEVHFPQLKRELLHEALTTFFKIRETPG
                     LKKKPTTSELLDWIKLLVSEDISPEALRSDDAKKLIPPLYGALLKNEQDVHLFEQLLF
                     LHRRNAR"
     gene            13341..14516
                     /locus_tag="VGE92_03130"
     CDS             13341..14516
                     /locus_tag="VGE92_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY1312838.1"
                     /translation="MLVRFFFLLRNAGVPVSITELLTLLEALQAGLGDLSGERFYYLA
                     RTCLVKDERYYDRFDRVFAVHFKGAEDLFATLQRELPEDWLAKMAVREFTDAEKAEIE
                     ALGGWEKLMETLRQRLEEQKERHQGGNKWIGTAGTSPFGAHGFNPEGVRVGQAGGRNR
                     SAVKVWDRREFANLDDKVELGTRNIKIALRRLRRFARRGAPDELALADTIDATARSGG
                     WLDIKMQAERRNVIKVLLLLDVGGSMDDHVKTCEELFSAARSEFKHLEHYYFHNFTYE
                     ALWRDNRRRFNEHIGTVELMRTYGTDYKLILVGDATLSPYEITQPGGSVEHWNAEAGS
                     VWLQRLTKHYPKFAWINPQPQSRWGHTASIEMTREMLEGRMYPLTLAGLDAAIDALT"
     gene            complement(14513..>14753)
                     /locus_tag="VGE92_03135"
     CDS             complement(14513..>14753)
                     /locus_tag="VGE92_03135"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY1312839.1"
                     /translation="YITGRNLELQPNERLVQAWRTADWKPGEYSIAGFAFLEEGAGTT
                     LVFDHRGFPDDAGEHLAHGWHVHYWTPLAKYLSRL"
BASE COUNT         2917 a         4546 c         4714 g         2576 t
ORIGIN      
        1 gcgtagaaca cccacgccgg gcgcccgagc cagagcgctg ctgcaatcac caggccccag
       61 ggcagcaacc aaaaccctag caacccgtcg aagtggccga cccatgccga aaaccttggc
      121 gagctgcccg ctgcagtgct ttgttgcagt atgcggagca cgatcaccca tccggcgtgc
      181 aagccgatcg cgacggcgat attgccggtc atcacacggg tcaggctcaa gatcaggccg
      241 acgaccaacc acgacacaaa agaatcgaac accagcgccg ggtggctgag cggcgcaaac
      301 gagcgcagca ataggtcgaa accgctgccc cagcctaatt cggccaccgg aatgctggcc
      361 ttggcgaaga agtgcagcac ggcgaatagc ggcgcgctga gcaaagcagc ggtccaaggt
      421 cccgattcgc gctcgatcgc gctgtgcatg gcgccgcgaa ataccgtctc ctcgatgagt
      481 gcaacactga tgccggaccc caatccgacc aagaaaatat gcagaaaatt cgcggctgag
      541 aggacgaatt gagcttgccg gacgcgcagc tccgtgccga gcaggaacgc cgcgccgacc
      601 ccggccgtcg caatgccgat caagccccac agcacgcaca ctcgcaagaa tcgccgccac
      661 ggcagcccga agccgaagtc gcgtttgcgt gtcagtcgta aatgccggca tagccagatg
      721 agttcagcca gcaaaatcag catggcaatg cggctcgcca cgcggtgaaa cgcccagggc
      781 gcgaagctcg aggagagttc gtaggcaggg tacgcgagcg ccgcgccgat cacgccggcc
      841 atcaggatgc aggctaagaa ccaagcaaag gcgcgcatat tctagcggat tcgtgcgaaa
      901 acgcgctttg cggcgacgat ggtggcgtcg atgtcggccg gcgtgtgtgc cgcagacaag
      961 aatcctgcct cgaacgccga cggcgcgaaa taaacaccct cggcgagcat gccgttgaaa
     1021 aaccgcttga atcgttccac atcgcaggcc atgactttgc tgtagcgatc gacgttggct
     1081 tcggccgtga agaaaagccc gaacatcccg ccgacctgat tggtcgcgaa cgcaatgccg
     1141 gcgtcgcggg cggactcggc gaggcgccgc gtcaactcgc cggtggcggc acttaagcgc
     1201 tcgtgaaatt gcggcgccga cagcaattcg agcgtggtga ggccggccat catcgccacc
     1261 gggttgccgg acaaagtgcc ggcctgatag accgggccca tgggcgcgag gtgttccatc
     1321 acgtcgcgtc gcccgccgaa tgcacccacc ggcattccgc cgccgatgat cttgccgagc
     1381 gtggtgaggt cgggccgtac gccgtacagc gcctgcgcac cgcccagggc gacgcgaaat
     1441 ccggtcatga cttcgtcgaa cagcaacagc gcgccgctcg agtcgcattc gcgccgcagc
     1501 gtgtcgagaa agtccggcgc cggcggcacg cagttcatat tgccggcgac cggctcgacg
     1561 atgacacagg cgatttgccc gccgatctga cggaacacct cacgcacctg ttcggcgtcg
     1621 ttgaagttca aagtcacagt gtagttggcc agcgccgccg gcacgccggg cgatgtgggg
     1681 acgcccaacg tcaatgcgcc cgagccggcc ctgatcaaca gcgaatcgga gtgcccatga
     1741 tagcagcctt cgaatttcac gatggtgtcg cgcccggtaa agccgcgcgc caagcggatc
     1801 gcgctcatgg tcgcctcggt gcccgagctc acgaagcgca ccaattccac ggaaggcatg
     1861 agctcgacga cgcggcgcgc aagcatggtt tcgagctcgg tgggcgcgcc gaaggaaagg
     1921 ccgtcggcgg ccaccgcctg cacggccgcg atgacttgcg gatgcgcgtg accgagaatc
     1981 atcggacccc aagagccgac gtaatcgatg aagcgacgcc cgtcggcggc cgtcaggtag
     2041 gcaccttgcg ccttcttgaa gaaaatcggc tcgccgccga cgccgcgaaa cgcgcgcacc
     2101 ggcgaattca cgccgcccgg aatgtacttg caggcttctt ggaacaggct catggcggga
     2161 ctctaaacag cgacagtgcc cggcggcaag ctgagccgca cttcgaagcc gccgccttgc
     2221 cggttgcagg cgctcgccga gccgccatgc gccttcatga cttgggcggt gatggcgagc
     2281 ccgatgcctt cgccgccggt gtcgcggtcg cgcgactcgg ccacccggta aaatggctcg
     2341 aagatccgtg tgaggtcggc ctcgggaacg ccgggtccgc gatcccgaac caaaatgacg
     2401 acgctctcgc cgcccgtgac caccacctcg accgcgccat ccggcgggct gtaacgcacg
     2461 gcattgcgca ggacgttttc gattgcgctg cgcaacagat cgcggttgcc gttgaccgcg
     2521 gcgtcggccg cgccctgcag ccgcacccgg caattcttca ccgctccttc gaaattcgca
     2581 tcgcgcacca cttcttcgat cacttcatcg aggtcgaagg tctcgcgcgc aaacggcgta
     2641 tcggtcccgg tcaggcgcgc cagcttcagc acctgactga tcagagcgtc gagacgctcg
     2701 atctcgcgct ccagccggtc caactgacgc gaggtgtcgg cgggcggctg gcgcgccagc
     2761 ccgaccgcga cgcgcattct cgcgagcggc gagcgcagct cgtgcgagat atcgcgcaat
     2821 aactgagtgc tggcgctgcg attcgcgcgc agctgatcgg ccatggcgtc gaaatcgcgc
     2881 gccagaaccg cgagctcgtc cttgcgcccc tcgagacccg cgctcacgcg cactcccgca
     2941 ttttgttgtt cgagattttc ggaggcgagg gtgcgtgcgc ctgcttggat gcgccgaatc
     3001 ggcgcactca ggtgctgcgc caaccaccag ctgatgagcg cgctcaccac cagcgcaatg
     3061 cacagaatgg tcgcggatat tcccggcaga ctgagcgcgc cgaaaatgct cggacggcgc
     3121 ggcaccagga ccacggtgta ggctgcgccg tcgggagcga cgatctgcgg cgccgcgcgg
     3181 ctcggacgga aattgccggg cggcggcggc tcgccgaacg cccgcggcgg ccgcgcgttc
     3241 tcgtcgcgat tggcgaactc gtcccgattg aagaattcca tgcggcgggc ggcgccctgc
     3301 gacaggcggc ggccaaggat gtcggtgcca tccggggcga cgatgaatag atcgcggtcc
     3361 gcgatcgaat ttttattggc gtcgagccaa tgcttgagtc cgacgattcc gccgcgcgcc
     3421 agtgcgtccg atgcctgaat ggcgacagcc ggccggcgtt gcaactcggg cgcctcgtac
     3481 tcgtgcgccg cgatggtgaa ggtgatggcg atactgccgc cgacgatgag cgccatcgcg
     3541 atccaaaaca acacgaaaat ccgcaggaac agactatgca tgagcccagg tgagggtgta
     3601 tcccgaaccg cggacgttct tgatttccgg atttttgccc gcctccagat ccagtttgcg
     3661 gcgcaggttg ctgatgtgag tgtcgatgct gcgatcgtag gcgacgagct tgcggcctag
     3721 cgcctgctca gtcatcgact cgcgcgagat cacctggcct gcggagcgca tgagaatttc
     3781 gagcacgcgg aattccgcgc cggtgagcgc caccgcggcg ccgccgacgt gcacttgatg
     3841 agttccggtg tgcagcgtga tcggaccgac cacgagttca tccgcgacgg cggacttgcc
     3901 ctggttgttg gcacggcgca ggatggcgcg aatgcgcgcg accagctcgc gcggattgaa
     3961 tggcttgctc aagtaatcgt cggcgccgag ctcgaggccg acgatgcggt cgacgtcatc
     4021 gccgcgcgcg gtgagcatca gaatcggggt cggataagct gcgcccagct tgcgcagcac
     4081 gtcgaggccg ccgaccgtcg gcaacatcac gtcgagaatc agaatttcaa agtgctcgac
     4141 ccgcaacgcg tcgagcgcgt cgccgccgtc gtgcacgctc ttcacgtcga agtgctcggt
     4201 gacgaggtat tcggtcagca tctgacatag ttcgcgatcg tcgtcgacca gcagcactcg
     4261 tgcttcgcgt ggggttggat ccatacgcct actgtggcac agtcgcgggc ccgccgagcc
     4321 ccccgctcat gaattcacaa ttcttaacag atcgttgcga tccgcgcggt acatgtttgc
     4381 acgcttcaag cttaggatgg tgccgtgaat tctcaactca agtattggag cgtacgaaca
     4441 tgaaatcgat tcgcaacgtg cttaaggcga cgctgctgac ggcaggcgcc gtgctgacgg
     4501 cggccaccag cctgtcgctt gcgacggccg ccgacgaagc ggcaaccacc accccgccgc
     4561 cgccgggtcc gcagggctgg caccatcacg ggccgatgca cctgctgagc cagctgggct
     4621 tgagtgcgac gcaaaagcag caggtcaagg acatcatgac ggcggcgcgt cccgaaatgc
     4681 agagcctgca cgaacagata catgccaatt ctctgaaact gcggcaaacg cagccgaccg
     4741 atccgaacta cagcagcatc gaggcgcagg tgagccaaac ccacggttcc ttgtcggcgc
     4801 agatgatgac gcagcaagcg gaggttcgtg ccaagctgtt caagatcctg acgccggcgc
     4861 agcaaacgca gctcgccgcg ctcgaagccg agatgcaggc taaacacggt gctcgaggcg
     4921 gcccccgcgg cccgggcccg gacgttgggg cgccgccatc ggagtaatcc aggcgtttgg
     4981 ctcgagggat tggtgtcagc gttgggttgc gccaatcccg tcttttttga gcagatcgat
     5041 cacgctgcct gaccctgcca ggtgccccgc gccgacgatg accagataat tcttatcgtc
     5101 gttcagcaag gcttcgattt tcggcaccca cttgcggttg cgcgctccca gaatggattg
     5161 atacaggccg gaatcttgct tcagatccga ctccaggtgg gtcgcgaacc aatgcgtgtc
     5221 gccgcgttgc caagcctgca ccatcgcgtc gacttccttg ggcaagtcat gggcctgctc
     5281 caagctcgat accaggtact cggcctgagt gtccagcgac atgttctcga acaatgagat
     5341 ctgatcgtgg atcgtttcga gacctgcgac gctcttgccg tcggtcttgg cgcgctggat
     5401 gaagtacatc tcgacgccgg cctcgggctg aaagccgagt tgcgtcaatt gcaattgcga
     5461 aatggcctcg gcggcgaacc agggggcgaa ttgatcgaag gtggagagct caatccccac
     5521 ctcgtgcgcc agagcatctg cacgtccgta gcgctccttg ccgaggaccg cgggcagggt
     5581 tttgccttcc ggcaaggtcg cactggcgag catttcggct tgcacctctg cagagccgct
     5641 gtcggcgaga ttcacttcca tgagcagcgc cttggcgttg ctgtaggcct ccaacaaggc
     5701 gggcgccaac gggtagtcgc tgaggcgcag cacgtgaatg gagccgagaa tgtacacggt
     5761 gttgtgcttg ccgcggactt cccagagcga atgcagcgcc gtgtcggcgc gggccgtgac
     5821 gcaaagcagc agaccgcaga gcgcgaggcg catcgaatgt cgttgcgggt ctagcgcgcc
     5881 accgactggt acatcggcaa cgactcgagc cagtgaaccg cggtgtcgat acgtccatcg
     5941 gcatgcaagt cgaagtgtcg ataggcaggc ggccgcgaat cgagctcgta gcggtcgctc
     6001 tttggcaaga attgcgcccc cgtcgacggg gttgcgtaca agcgcacggg gccgcgttga
     6061 ccgtcgtacg cctggtggac atgtccccag acgacggcgc gtacgtgctc atgcgcatcg
     6121 atcacgcgcc aaaagtcctc cggcgcggcg agccccacgg tgtccagcca gcggctgccc
     6181 atggtaatcg gatgatggtg caaacagacc atggcgtgtt ttggcgaagc ggcgagcgtc
     6241 gcatcgagtc gctccagttc ggccggcgtg aggcgcccac cgacgtgccc gggatcgtag
     6301 ctgtcgagca tgatgaactg ccagccgtcg gcttcgtgcg agccgcaaat ctgaaacggt
     6361 gcgccggcga gctcgcggcg catggcctcc ggctcgtcgt ggtttcccgg gatgcacagc
     6421 accggcttct tgagattgcc gaagatgctg cgaaagcgca gatagccgga actgtcgtcc
     6481 tgcaccagat cgccggtcac caacacagcc gaaaaatccg gaacgcgcgc gaacgcagca
     6541 tcgaggaccg cgtgcaagct cgaatcggtt tccactccac gcagccggcc ggtcgcgctg
     6601 ccgtagagat gagggtcggt gatttgtaga atttgcatgt gggaaatggt agcaaagcac
     6661 cccaacggtg acggtgaact gggtcatgac ggcggtcgaa ctggttatac ataaacagac
     6721 ggcatttgtg tgaacgcgtt ccgtcccgcc gctcaaatcg acgcgccatt gctggcgagg
     6781 ggcgcatcga ggacaatacc gtgccggatt ccattcactt ttgttaaatc aaagaagtcg
     6841 cctatgccta cccgccgcga gctcgccaat gccattcgtg ctttatcgat ggatgccgtc
     6901 cagaaggcca attcgggtca tccaggcgcg cccatgggga tggccgacat cgcggaagtc
     6961 ctgtggcggg atgtccttaa gttcaatccc ggcaatccta gctggtggga ccgcgaccgt
     7021 ttcgtgctgt cgaacggcca tgcctcgatg ctgctgtact cggtgctgta tctgacgggc
     7081 tacccgttgt cgatcgatga tatcaagaac tttcgccaat tcagttccaa gaccccgggc
     7141 catccggagc gagaagtgca tctcgggatc gaaaccacga ccgggcctct gggccagggt
     7201 ttcgccaatg gcgtgggcat ggcgttggcc gagcgcgcac tcgccgccac cttcaatcgt
     7261 cccggccaca acatcgtcga tcatcacacc tacgtgtttt tgggcgacgg ctgcatgatg
     7321 gaaggaatct cgcacgaagt ggcatcgttc gccggcgtgc agcgcttagg caagctcatc
     7381 ttggtgtacg acgacaacgg catctcgatc gatggcaagg tggtggggtg gttcggcgac
     7441 cacaccgcag aacgcttcga ggcctatggc tggcacgtgc agccccgcgt cgacggccac
     7501 aacagcgacg ctgtcgcggc cgcgttggct gcggcgcgcg ccgaaaccgg tcggccgtcg
     7561 ctgatttgcg cgcaaaccat catcggctgg ggagcgccga acaagcaggg cactgcatcg
     7621 atgcacggcg aggccatggg gaacgaggaa attgccgcca cgcggttgaa cctcaagtgg
     7681 agctcaccgc cgttcgagat accggccgat atccgcgcgg cctgggacat gcgcgacaaa
     7741 ggtgcgcggg tcgagcagga gtggcgcacg cggtttgccg cctacgccag cgaatttccc
     7801 gcgttggcag ccgaactcga gcgccgcatc gaaggcaacc tgccgagtgg attcgccgac
     7861 ctcgtgagtg cgtatatcgg acgcacccag gtcgagggaa aggccctcgc gacccgccaa
     7921 tcctcgcaag cggcgttgaa cgccatcggt ccatcgatgc ccgagcttct gggaggatcc
     7981 gcggatctca cgccctcgaa cggcacgctg cgcaaggact ctgtggccct gaccccggac
     8041 tcgccgggcg gcaactacgt gcatttcggc gtgcgcgaat tcggcatgtc ggcgattctg
     8101 aacggcatcg tgtgccatgg cggcatcatt ccgtacgccg ggacgttcct gactttctcc
     8161 gactacgcgc gcaacgccgt gcgcatggcc tgcctgatgc gcttgcgcgt ggtcttcgtc
     8221 tatacccacg attccatcgg tttgggcgaa gacgggccga cgcaccagcc gatcgaacac
     8281 ttggcgagtt tgcgcgacat cccgcacatg aacctgtggc ggccctgcga tgcggtagag
     8341 accgcgacct cgtggggagt ggcgctcgag catcgcgatg gcccgacgtg cttgattttc
     8401 acgcggcaag ccgtggtgca cgaggcacgc gaagcggcgc aggtggcgaa tatccggcgc
     8461 ggcggctacg tgttgatcga ttccgcgggg ccgccggaag ccatcgtgat cgccacgggt
     8521 tccgaagtcg gcatcgccgc cgaagcggtg cgcaccctga cggccaaagg caggaaaatt
     8581 cgcttggtgt ccatgccgag caccaacacc ttcgacagcc aagacgaggc gtataagcaa
     8641 agcgtgctgc cgtcggcggt cacgcgtcgc gtcgcggtgg aagccggcgt gagggcgact
     8701 tggtggcagt acgtcggctt gcagggagaa atcgtcggca tcgatcattt cggcgcctcg
     8761 gccccggcaa aggagctgtt caaagcctac ggcttcacgc cgcagcacgt gatcgaggcg
     8821 atcgagaaag ttctagctaa gtaagatcaa acgaggatta aaagacgatg gcaatcaagg
     8881 tggctatcaa tggttatggg cgcatcggcc gcaatattct gcgggcgctg tacgagggca
     8941 agcacgcggg cgaaatcaag atcgtcgcga tcaatgattt gggcgatgcc aacaccaacg
     9001 tgcatttgac gcgcttcgat accgtgcacg gccgcttccg cggcgacgta catgtcgacg
     9061 gcgactcgat ggtggtaaac ggcgaccgca tccgcgtggt cgcgcagcgc gacccctcga
     9121 agctgccgtg gggcgaactc ggcgcggatt tcgtgttgga atgcaccggc ttgttcacca
     9181 gcaaggccaa ggcgtcggcg cacattgcgg cgggcgccaa aaaggtgatc atttctgccc
     9241 cgggcggcga tgatgtcgac gcgaccatcg tatatggcgt caatgacggc gtactgaagg
     9301 caagccatag cgtcatttcc aacgcctcgt gcaccaccaa ttgcctggcg ccgctggtga
     9361 aggtgctgca cgagaaagtg ggggttctgt cgggagtgat gaacacgatc cactcctaca
     9421 ccaacgacca agtgctgacc gatgtgtacc actcggattt gcggcgtgcg cgctcggcga
     9481 ccatgtcgat gattcccacc aagaccggcg ccgccgccgc ggtcggcctg gtgctgccgg
     9541 aactcaaagg caagatcgac ggcttcgcga tccgggtgcc gaccatcaac gtatcgctgg
     9601 tcgatttgag cttcgtcgcg gcccgcaaga cttcgatcga ggaaattcac ggcgcggtca
     9661 aggcggccgc cgccggcgct ctgaaaggga ttctggctta caacgacgcg ccgctggtgt
     9721 ccaccgattt caatcacgat ccggcatcct cgacctacga tgccacgctc accaaagtca
     9781 tcgaaggcaa cctggttaag gtgtgttcct ggtacgacaa tgagtggggg ttctcgaacc
     9841 gcatgctcga caccaccgtg gcctggtcga aagccactta gaaggcgctc ttgaagttaa
     9901 tcaaaatggc gagcctggat ctgcgtgaca agcgcgtgtt gatccgtgaa gatctgaacg
     9961 tgccgatcaa agatggcgcg gtgagcagcg atgcacggat tcgcgcggcg ctgccgacca
    10021 ttcaggcggc actgacggcg cgagcccggg tgctcgtgat gtcgcatctc ggcaggcccg
    10081 aggagggcaa gtatgcggcg gagttttcgc tcgcgccggt tgccaaacga ttggcggagc
    10141 ttctcggcgt gccggtgaag ctgcagcaga actggctcga cggggtggac gttcaccccg
    10201 gcgaagtcgt attgctcgag aatgtgcgct tcaatacggg cgaaaaaaag gacagcgagg
    10261 agcttgcgcg caagatggcc gcgctctgcg acgtgtatgt gatggatgct ttcggtacgg
    10321 cgcaccgcgc cgaggcgagt actcatgggg tggcccgcta cgcgccggtg gcctgcgccg
    10381 gcccgctgtt ggtcagtgaa ctgtccgcgc tcgagaccgc gctcgagaag cccgcgcgtc
    10441 cgctgctcgc catcgtcgca ggttccaagg tatcgaccaa acttacggtg ctcgaatcct
    10501 tgctcggcaa ggtggatcaa ctgatcgtcg gcggcggcat caccaatact tttctcgcgg
    10561 ccttgggctt caacgtcggc aagtcgctgt acgagcccgc gatgctcgat atttgcaaac
    10621 gcctgctgga gcagtcgagt aaacgcggca tcgtcattcc gatgcccacc gatgtcgtgg
    10681 tcgccaagga gttctctgcc aaagcggaag ccgacgtcaa gccggtcggc gccgtgtcgc
    10741 cggaggaaat gatcctggat atcggacccg attcagccga agccatgagc gagttggtcg
    10801 cctcggcggg cacgattctg tggaacggtc cggtgggcgt gttcgagttc gagcaattcg
    10861 gcgaaggtac ccgcacgctt gcgacggcga tagcgcgcag caaggcgttt tcgctggccg
    10921 gcggcggcga cacgcttgcg gccatcgaga aatacggcat cgaggacagc atttcctata
    10981 tatcgacggg cggcggcgcc ttcttggaat tcgtcgaggg caaaaagctg ccggcggtgg
    11041 agatactcga gcagcgcgcc gcatgagtgc tttgcgccgt accaagatcg tcgccacctt
    11101 gggtcccgcc acggatgatc cggctgtgct cgccgatatc gtgcgcgccg gtgtggatgt
    11161 ggtacggctc aatttctcgc acggcgcagt ggctgatcac gcgcgccggg tcgaactggt
    11221 gcgcgaggcc gcacgcaagg cggggcgcta tgtgggcgtg ctcggcgacc tgcagggacc
    11281 caaaatccgc atcgatcgct ttaccacggg caaggtgcag ctggccgacg gtgcagaatt
    11341 cacgctggac gcggcgctcg ccgtcgatgc cggcaccgaa cacagcgtcg ggatcgccta
    11401 caagaaatta ccgacggacg tcttcccgga cgacgtgctg ctgctcaatg acggacagat
    11461 cagtttgcag gtcctgagca tcgacggacc gcgcatcaag acgcgcgtat tggtgggcgg
    11521 cgagctcggc aacagcaagg gcatcaaccg gcagggcggc ggcctgtccg caggcgcact
    11581 cacggacaag gatcgagccg acatccgcac ggcggcgtcg ctcaaggtgg attacctcgg
    11641 agtctccttt ccgcgcgacg cttcggatat ggacgaagcg cgcaagctgc tgcgcgatgc
    11701 cggcggccat gggttgctgg tggcgaaaat cgagcgcgcc gaggccatca agaacctggc
    11761 ggaggtggtg cgggcgagcg acggggcgat ggtcgcgcgt ggcgacctcg gcgtcgaaat
    11821 gggttatgcg gaactcgcag gcctgcagaa gcaaatcatc caggaagcgc gccatcagaa
    11881 ccgcgtcgtc atcatggcga cgcagatgat ggaatcgatg atcaccaaca cgattccgac
    11941 gcgtgccgaa gtttcggacg tcgccaatgc cgtcatggac ggcacggatg cggtgatgct
    12001 gtcggccgaa accgcctccg gcaagcatcc ggcgaaggtg gtcgaggcca tgtcgcaaat
    12061 catcgtcggc gccgaaaagt atcaagtcgc ccacgtgcgc atcaggcagc gcagcagcgg
    12121 ttatttcggg cacaccgacg aggccattgc ggcggcggtc atgtacaccg cgaatcactt
    12181 gcatgtgaaa gccatcgtcg ccttgaccga gtcgggttcg acctcgctgt ggatgtcgcg
    12241 ggtgcgctcg gacattccga tctacgcctt cacccggcac gaagcgacgc gttgccgggt
    12301 gacgctgtat cgcggggtct acccggtcgc ctatgacatt ccggagaagg gcgaagcggt
    12361 gtatttctcg atattccgcc tgctgctgga tctcaatttg gtggcggaag gcgattcgat
    12421 catcctcacc aagggcgaat tgagcggcgt ctcgggcggc accaattcca tgcagatact
    12481 gcaagttacg cgcaagtaaa tgccgcagga atttcgcggt accgatcgtt acattgccac
    12541 cgaggaattg atggccgccg tgaacgccgc catggtgttg tcgcgtccgc tgctgatcaa
    12601 gggcgaaccc ggcaccggca agacgctgct cgccaacgag atcgcgcgat ccttgaacaa
    12661 gccgttcttc gaatggcaca tcaagtcgac caccaaggct cagcaggggc tatacgaata
    12721 cgacgccgtg tcacgcctgc gcgattcgca gctcggcgat ccgcgcgtca aggacattca
    12781 caactacatc gtgaaaggcg acttgtggga ggcattcgat tgcgaggtgc agccggtgct
    12841 gctcatcgac gagatagaca aggcggatat cgagttcccg aacgacctgc tgcgcgaact
    12901 cgaccgcatg gaattctacg tgtacgagac tcacgaaacc gtgcgcgcca aacaccgtcc
    12961 ggtgatggtg atcaccagca acaacgagaa ggaactgccc gacgcattcc tcagacgctg
    13021 tttctttcac tacatccgct tccccgatgc cgccaccatg acgcagatcg tcgaggtgca
    13081 ttttccgcag ttgaaacgcg agctgctgca cgaggcgctg acgacattct tcaagattcg
    13141 cgaaactccg ggattgaaga agaaaccgac tacttccgag ctgctcgatt ggatcaagct
    13201 gctggtgagc gaggatattt ctcccgaggc gttgcgcagc gacgatgcga agaaactcat
    13261 tccaccgctg tatggcgcac tgctcaagaa cgagcaggac gtgcatctgt tcgaacaatt
    13321 gctgttccta caccgccgga atgctcgtta gatttttctt tctgctgcgc aacgccggcg
    13381 taccggtatc catcaccgaa ctcctgacgc tgctcgaggc attgcaagcc ggcctcggtg
    13441 atttgagcgg cgagcgcttc tattatctgg cgcgcacctg cctggtgaag gacgagcgct
    13501 actacgatcg attcgatcga gtctttgcag tgcatttcaa gggcgccgaa gacctgttcg
    13561 caacgctgca gcgggagttg cccgaggatt ggctcgccaa aatggccgta cgcgagttca
    13621 ccgacgccga aaaagccgag atcgaggcgc tcggcggatg ggaaaaactc atggaaacgc
    13681 tgcggcagcg tctcgaagag caaaaggaac gccaccaggg cggcaacaag tggatcggca
    13741 cggcgggaac ctcgccgttc ggcgcgcacg gtttcaatcc ggaaggcgtg cgggtgggtc
    13801 aggccggcgg ccgcaaccgt agcgcggtga aggtctggga ccggcgcgaa ttcgccaatt
    13861 tggacgacaa ggtcgaactt ggaacgcgca acatcaagat cgccctgcgc cgcctacggc
    13921 gttttgcgag gcgcggcgcc ccggacgaat tggcgctcgc cgacaccatc gatgccacgg
    13981 cgcgctccgg cggatggctc gacatcaaga tgcaggccga acggcgcaac gtcatcaaag
    14041 tgctgttgct cctcgacgtc ggcggctcca tggacgatca cgtgaagacc tgcgaggagt
    14101 tgttctccgc ggcgcgcagc gagttcaagc acctggagca ctactatttt cacaatttta
    14161 cgtacgaggc gctgtggcgc gacaatcgac gccgcttcaa cgaacacatc ggcaccgtcg
    14221 aattgatgcg cacgtacggc accgattaca aattgattct ggtgggcgat gcgacattaa
    14281 gcccgtacga aatcactcag ccgggcggca gcgtcgagca ctggaatgcc gaggcgggca
    14341 gcgtctggct gcagcgcctg accaagcact acccgaagtt tgcctggatc aatccgcagc
    14401 cgcagtcgcg ctgggggcac accgcctcga tcgaaatgac gcgggaaatg ctcgagggaa
    14461 gaatgtaccc gctcacgctc gccgggcttg acgctgcgat cgacgcactg acctaaaggc
    14521 gcgacagata cttcgcgaga ggcgtccagt agtgcacgtg ccagccgtgg gccaggtgtt
    14581 cgcccgcatc atcggggaag ccacggtggt cgaacacaag cgtggtcccg gcgccttctt
    14641 ccaagaatgc gaagcctgcg atcgaatact cgccgggttt ccagtcggcg gtgcgccagg
    14701 cctgaaccag gcgctcgttc ggctgcaact ccaggttccg gcccgtgata tag
//
LOCUS       DASWZT010000116         8350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_39671, whole genome shotgun sequence.
ACCESSION   DASWZT010000116 DASWZT010000000
VERSION     DASWZT010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8350
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39671"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1873)
                     /gene="ggt"
                     /locus_tag="VGE92_03140"
     CDS             complement(167..1873)
                     /gene="ggt"
                     /locus_tag="VGE92_03140"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY1312840.1"
                     /translation="MTCALAVVLAAAPAAFADDTPVAAAKPLLPARPAIAHQAAIASA
                     YPLASQAGQEVLAAGGNAFDAAVAVSAALAVVEPSSSGLGGGGFYLLHRQSDGFETMV
                     DAREKAPGAASRDMYLDKAGNEIDKASISGALSAGIPGEPAAFEFLARKYGKLPLKQS
                     LQPAIRLARDGFPLYARLQGGIRYKQSGLARSPDAAKAFLTAEGGVPEVGALIKQPDL
                     ANTLEAIANQGARGFYQGRVAADLVAGVRADGGIWTLQDLEAYRIIERKPLVGTYHGA
                     RIVSASPPSSGGIAVLDALNILAGFDLHRYDSATHKHLVIEAMRRAYRDRAIYLGDPD
                     FVKMPLAQLSSLDYAAGQRSSIRTDKAMPSSMLPGIESEPAGMQTTHFSVLDADGNRV
                     AATISINLFFGSGYVPPKTGVLLNDTMDDFSTKPGRPNEFGLVGAAANAIAPNKRSLS
                     SMSPTFVETPKGLMILGSPGGSYIISMVLLGTLNYLDGMDAAQIVKYPHYHHQYLPDE
                     VDYEQGAFSEAEIKELESMGHTLKVSGRQWGNMQVITWDFASGKVEAASDPRGEGVGL
                     VY"
     gene            complement(1909..2394)
                     /gene="coaD"
                     /locus_tag="VGE92_03145"
     CDS             complement(1909..2394)
                     /gene="coaD"
                     /locus_tag="VGE92_03145"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004641605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEY1312841.1"
                     /translation="MHKRTAVYPGTFDPITNGHHDLVRRAASIFDRLVVAIAANPNKA
                     PMFTLEQRVDMARRVLADSHNVEVMGYAGLTVEFARQNGLAVIVRGLRAVSDFEFEFQ
                     LANMSRHLSPEIESVFMTPQEQYTFISSTLVREIAVLGGNVSEFVHPLVEIELKRHRK
                     L"
     gene            complement(2387..3001)
                     /gene="rsmD"
                     /locus_tag="VGE92_03150"
     CDS             complement(2387..3001)
                     /gene="rsmD"
                     /locus_tag="VGE92_03150"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEY1312842.1"
                     /translation="MTTHKTESNGAGGRNSVRIIGGGWRGRRVTFPDIPGLRPTPDRV
                     RETLFNWLQPAIVGARCLDLFAGSGALGLEALSRGAKHSVFVETALPASRALQEQLIR
                     LGGASRGQVVEMGAARYLRSPPQPFDIVFLDPPFGQDALAEYVPLLDQGVWLKLGSLV
                     YLENEKAAGVPGLPSHWQILKSKSAGEVGYHLARVSSAGLSLNA"
     gene            3022..3984
                     /gene="ftsY"
                     /locus_tag="VGE92_03155"
     CDS             3022..3984
                     /gene="ftsY"
                     /locus_tag="VGE92_03155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEY1312843.1"
                     /translation="MTNQADPPVRGFFKRLRAKLNQGPAWLTTDISELLPGRKIDAQI
                     LDELETRLITADVGAEATRRILEELRKRVARKELNDVDALLKALHDAMFDILRPVEQP
                     LVIDRGIKPFVILVVGINGAGKTTTIGKLAHRLLAEGFSVMLAAGDTFRAAAREQLEV
                     WAERNKVPLIAQQDGAEPAAVIFDGLQAARARNVDVLIADTAGRLHTQTHLMDELKKV
                     KRVLARLDAGAPHEVLLVLDGTIGQNAIAQAEVFNQGLGVTGLVITKLDGTAKGGVVL
                     AIAQKLKIPIRFVGVGEQTEDFGVFNASEFVAALLKSEPAGADA"
     gene            3981..4661
                     /gene="ftsE"
                     /locus_tag="VGE92_03160"
     CDS             3981..4661
                     /gene="ftsE"
                     /locus_tag="VGE92_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017903464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEY1312844.1"
                     /translation="MIRFDQVHKRYPTGREALAGVSFKIDAGELAFLTGHSGAGKSSI
                     LKLIALIERPTRGQVFVNNQNISGIKPRGIPQFRRGLGVVFQDNKLLHDRPVADNVAL
                     PLIIAGVPKREIDKRVRAALDQVGLLGTEKNRPLELSSGEQQRVGIARAVVAKPALLI
                     ADEPTGNLDPDLALEIMKLFKRFNEVGVTVVIATHDVHLIEQFAARRIVLGEGRVIDA
                     GGVRPGAP"
     gene            4658..5578
                     /gene="ftsX"
                     /locus_tag="VGE92_03165"
     CDS             4658..5578
                     /gene="ftsX"
                     /locus_tag="VGE92_03165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEY1312845.1"
                     /translation="MNIQAYFARHAQTLVGSLGRIVGHPFATLMTMGVVAVALALPLL
                     LNVFLANGRTVIGSWNDAFDLSVYMDKRADSARATGLAKQLRQRDDVAAVRVIPADQA
                     LSEFRDESGFGKALDALSDNPLPDTLVVTPTLLASTPQGTEALKTAIAAMADVHAVQL
                     DTEWVKRLHAMLDILRRVVLLTACLLGAGVVLIVGNTIRLDILNRRAEIEVMKLVGAS
                     DGFARRPFLYSGIWYGLGGGLMALTLVAIAVAVLARPVDELAKLYGSPFHLQGLSLPL
                     GASVLALAVGLSWFGSWLAATRHIRGIDPS"
     gene            5683..6552
                     /gene="rpoH"
                     /locus_tag="VGE92_03170"
     CDS             5683..6552
                     /gene="rpoH"
                     /locus_tag="VGE92_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007939338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEY1312846.1"
                     /translation="MTTALVAKQAGGLVFAGPVGSLDSYIDRVSQIPVLSKEDEVALA
                     TRFRSEADLDAARQLVLSHLRFVVHIARGYLGYGLPMGDLVQEGNVGLMKAVKRFDPT
                     VGVRLVSFAVHWIRAEIHEYVLRNWRLVKVATTKSQRKLFFNLRKMKKNLAWLSEAET
                     AAVARDLGVDVADVREMEQRLSARDMSFDPTPESDEEEAYSPAMYLPSSNADPAIEVE
                     NEEWEEDSTERLSLALEKLDERSRSILKRRWMTDDKATLHELADEYGISAERVRQVES
                     NAISKLKGLMAAA"
     gene            6559..7605
                     /locus_tag="VGE92_03175"
     CDS             6559..7605
                     /locus_tag="VGE92_03175"
                     /inference="COORDINATES: protein motif:HMM:NF019193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase domain-containing
                     protein"
                     /protein_id="HEY1312847.1"
                     /translation="MSAPLGVIEGFFGKPWTDTARLACAEFLRPRGFDFYVCAPKSDR
                     FLRRQWADPFPAPALAQLCELSSQIRERAVRFGIGLCPYDIHRNYDAAARAALRAKVA
                     QLDEIGTELLCILFDDMPGSFAGLAPLQARVTADITEWSSAAAFIVCPTYYSDDPILA
                     RVFGPAPQNYLQDLGRGLDPAVDVFWTGPEVCSSGYPDEHLEDVATRLGRRPFIWDNH
                     IANDGKLRCSHVYLDPHSSGWSLNRRLAAGLAVNPMNQPGLSRIALASYAAGAADAAL
                     ESHVRSVCSAPLGAALLGDVGLFQGRGIAALDAAGRSALAEKYRRFEPQWCAREIIAW
                     LEGEYEFDPNCLTQ"
     gene            7612..>8350
                     /locus_tag="VGE92_03180"
     CDS             7612..>8350
                     /locus_tag="VGE92_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312848.1"
                     /translation="MARRVAPVWLMGLTNAVFGMYGGILVISVPQLLSAQHVPEATIA
                     SMTAVMLSPGFWTFLVSPVLDVRFSRRWYSAFTAMVAAALLVVALLNLRNLALAEILL
                     VTGYFFANLYQSALGGWLASITTKEEESKLSVWVTVGNIGTGGAMALMTGEIVRNLPP
                     LTAALLLGALLLLPTAVFPWMPAPGPDRRLARESFPQFFGEVVSLLKQREVLIAILLF
                     IAPAATFSLTNFLGGLGADFHASTHVVG"
BASE COUNT         1489 a         2642 c         2655 g         1564 t
ORIGIN      
        1 gcacgacgct gcgcgattac gtgaatgcag acggcgcgcc gggatatttt cgccagaaat
       61 tattcgtcta cgaacgcgcc ggcgcggcgt gtcgcgtctg caaaaccgtc gtcaggcaat
      121 tcgtccaggg ccagcgctcc acgtattggt gcgcggtgtg ccagcgctag taaacgaggc
      181 ccacgccttc gccgcgagga tcggatgccg cttcgacctt gccgctcgca aagtcccagg
      241 tgatgacctg catgttgccc cactgccggc cggacacctt gagcgtgtgg cccatcgact
      301 ccagctcttt gatttccgcc tcgctgaaag cgccctgttc atagtcgact tcgtcgggca
      361 ggtattgatg atgatagtgc ggatatttga cgatctgcgc cgcgtccatc ccgtccagat
      421 agttgagcgt gccgagcaac accatgctga tgatgtagct gccgcccggc gagcccaaga
      481 tcatcaagcc tttcggggtc tcgacgaatg tcggcgacat gctcgagagg ctgcgcttgt
      541 tcggcgcgat ggcattggcg gcggcgccga ccaggccaaa ctcattgggc ctgccgggct
      601 tggtggagaa gtcgtccatg gtgtcgttga gcagcacgcc ggtcttcggc ggcacatagc
      661 cgctgccgaa aaacagattg atcgagatcg tcgccgccac ccgattgccg tcggcatcca
      721 acacggaaaa atgcgtggtc tgcatgcccg cgggttctga ttcgatgccg ggcagcatgc
      781 tcgagggcat ggccttgtcg gtgcgaatgc tgctgcgctg tcccgcggcg tagtcgaggc
      841 tcgagagctg cgcgaggggc atcttgacga agtccggatc gcccagataa atcgcccgat
      901 cgcgataggc gcggcgcatg gcttcgatca ccaagtgttt atgcgtcgca gagtcatagc
      961 gatgcaaatc gaaaccggcc aggatgttga gcgcgtcgag gaccgcgatg ccgcctgagg
     1021 agggcggtga cgccgagacg atgcgcgccc catgataggt gccgaccagc ggcttgcgtt
     1081 cgatgatccg atacgcttcg aggtcttgca gcgtccaaat gcctccatcg gcgcgcacac
     1141 cggccaccag atccgccgcg acccggcctt ggtaaaaccc ccgggcgcct tgattggcaa
     1201 tggcttcgag ggtattcgcg agatccggtt gcttgatgag cgcgcccacc tccggaacgc
     1261 cgccctcggc ggtcagaaag gctttcgcgg catcggggga gcgcgccagc ccggattgct
     1321 tgtagcggat gccgccttgc aggcgggcat atagcgggaa accgtcgcgc gccaatcgga
     1381 tcgcgggttg caagctttgc ttcaagggca gcttgccgta cttgcgcgcc agaaactcga
     1441 acgccgcggg ctcgccggga atgccggccg acaaagcccc ggaaatcgaa gccttgtcga
     1501 tctcattgcc ggctttgtcc aggtacatgt cgcgggatgc tgcgccgggc gccttttcgc
     1561 gcgcatccac catggtctcg aagccgtcgg attgccgatg cagcaaataa aacccgccgc
     1621 cgccgaggcc ggagctcgag ggttcgacca ccgcgagtgc cgccgacacc gccacggctg
     1681 cgtcgaaggc gttgccgcct gcggcaagca cttcctggcc cgcctggctc gcgagcggat
     1741 aggcgctcgc gatggccgcc tggtgcgcga tcgcaggtcg tgccggcagc aacggcttcg
     1801 ccgccgcgac gggcgtatcg tcggcaaacg ccgccggcgc cgccgcaagc acaacggcca
     1861 acgcgcaggt caatgcgcgc gcgccgcacg ctacgaaagc tgacctgatc aaagcttgcg
     1921 gtgccgcttg agttcgatct cgaccagcgg gtgcacgaac tcggacacat tgccgcccag
     1981 caccgcgatt tcgcgcacca gagtcgagga aataaaggtg tactgctcct gcggcgtcat
     2041 gaatacggat tcaatttccg ggctgagatg gcggctcata ttcgccagct ggaactcgaa
     2101 ctcgaagtcc gacacggcgc gcaaaccgcg cacgatcacc gccaaaccgt tttgccgcgc
     2161 gaactcgacg gtcaagcccg catagcccat cacctcgacg ttgtgcgagt cggcgagcac
     2221 ccgccgcgcc atatccacgc gctgttcgag ggtaaacatg ggcgccttgt tgggattggc
     2281 ggcgatcgct accaccaggc gatcgaaaat gctggccgcg cgccgcacca agtcgtgatg
     2341 accattggtg atcggatcga aggttcccgg atagaccgcc gttcttttat gcatttaagc
     2401 tcaggcccgc gcttgagacg cgcgccaaat gataccccac ctcgcccgcg gacttcgatt
     2461 tgagtatctg ccagtgagac ggcagcccgg ggacgcccgc ggccttttcg ttctcgaggt
     2521 ataccagcga tcccaacttg agccacacgc cttgatccag caggggaaca tattctgcca
     2581 gggcatcctg gccgaacggc gggtcgagga acacgatgtc gaacggctga gggggcgaac
     2641 gcaggtagcg cgcggcgccc atttccacaa cctgcccccg gctcgcgccg cccaggcgga
     2701 tcagttgctc ctgcagtgcg cgcgatgcgg gcaacgccgt ttcgacaaat accgagtgct
     2761 tcgctccgcg cgacaatgcc tcgagtccga gcgcgccgga gcccgcgaac aagtccaggc
     2821 aacgcgcccc gacaatcgcc ggctgcagcc aattgaacag cgtttcgcgc acccgatcgg
     2881 gcgtcggccg aagcccggga atatccggaa aagtgacgcg ccggccgcgc caaccgccac
     2941 cgatgatccg cactgaattg cggccgcccg cgccgttcga ttccgtcttg tgtgtggtca
     3001 ctcccggtat cataccctcc catgacgaac caagccgatc cgccggtgcg cggttttttc
     3061 aagcgcctgc gcgccaaatt gaaccaaggt cccgcatggc tcaccaccga catcagcgaa
     3121 cttctgcccg gacgcaagat cgatgcgcag atcctggatg aactcgagac gcgcctgatc
     3181 acagccgatg tgggcgcgga ggcgacccgc cgcatcctcg aggagctgcg caagcgcgtg
     3241 gcgcgcaagg aactgaacga cgtggacgcg ctgctcaagg ccttgcacga cgccatgttc
     3301 gatattctga gacccgtcga gcagccgctg gtcatcgatc gcggcatcaa gccctttgtg
     3361 attctcgtgg tcgggatcaa cggcgccggc aagaccacca ccatcggcaa attggcgcac
     3421 cggctattgg ccgagggctt cagcgtcatg ctggctgccg gcgacacctt tcgcgccgcg
     3481 gcgcgcgagc agctcgaggt ctgggccgag cgaaacaagg tgcccctgat tgcccagcag
     3541 gacggcgccg agcccgccgc ggtgatattc gacggcctgc aggccgcgcg cgctcgcaat
     3601 gtcgatgtgc tgatcgccga caccgccggg cgtctgcata cccagaccca cttgatggac
     3661 gagctgaaga aggtcaagcg cgtactggca cgcctcgatg cgggcgcgcc gcacgaagtg
     3721 ctgctggtgc tcgatggcac catcggccag aacgccattg cgcaggccga ggtgttcaat
     3781 cagggactgg gcgtgacggg attggtcata accaagctcg acggcacggc caagggaggc
     3841 gtggtgctcg ccatcgcgca aaagctgaaa attcccatcc gctttgtcgg ggtcggcgag
     3901 caaaccgagg atttcggcgt gttcaacgcg tcggaattcg tagcggcgct gctcaagtcg
     3961 gagcccgccg gcgctgacgc atgattcgtt tcgaccaggt ccacaagcgc tatccgaccg
     4021 gccgcgaggc gctcgccggc gtgtctttca agatcgacgc cggcgagctg gcttttttga
     4081 ccggtcattc gggtgcgggc aagagcagca tcttgaaatt gatcgcactc atcgagcgcc
     4141 cgacgcgcgg ccaagtattc gtcaacaatc aaaatatttc gggcatcaag ccgcgcggca
     4201 tcccgcaatt ccgccgcggc ctgggagtgg tctttcaaga taacaaactg ctgcacgacc
     4261 ggccggtcgc cgataacgtg gcgctgccgt tgatcatcgc cggcgtgccg aagcgcgaga
     4321 tcgacaagcg agtgcgcgcc gctctcgatc aggtgggcct gctcggcacc gagaagaatc
     4381 gtcccttgga actgtcgagc ggcgaacagc agcgcgtcgg catcgcgcgc gccgtggtcg
     4441 cgaagccggc gctgctcatt gccgatgaac ctacgggcaa cctcgatccg gatctcgcgc
     4501 tcgagatcat gaaattgttc aaacgcttca acgaagtcgg cgtgacggtg gtgatcgcca
     4561 cccacgatgt gcacctcatc gagcaatttg cagcgcgccg cattgtcttg ggcgagggcc
     4621 gggtcatcga cgccggcggc gtgcggccag gcgcgccgtg aatatccaag cgtatttcgc
     4681 ccggcatgcg caaaccctgg tgggatcgct cggccggatc gtcggtcacc cgttcgcgac
     4741 gctgatgacg atgggcgtgg tggccgtcgc cctcgcgttg ccgctgctgt tgaacgtgtt
     4801 cttggcgaat gggcgcacgg tgatcggcag ctggaacgat gccttcgact tgtcggtcta
     4861 tatggacaag agggccgaca gtgcgcgcgc gaccggcctc gccaagcagc tgcgccaacg
     4921 cgatgacgtc gcagcggtgc gcgtcatccc ggctgaccag gcgctctccg agtttcgcga
     4981 cgagtcgggg ttcggcaagg cgctcgatgc attgagcgac aatcccctgc ccgacacctt
     5041 ggtggtcacg ccgacgctgc tcgcgagcac gccgcaaggc acggaggcgc tgaagacggc
     5101 catcgccgcc atggccgatg tgcatgcggt gcaactcgac accgagtggg tcaagcgcct
     5161 gcacgcgatg ctcgacattt tacggcgcgt ggtgttgctc accgcctgcc tgttgggcgc
     5221 cggggtcgtg ctcatcgtcg ggaacaccat ccggttggat atcttgaacc ggcgcgccga
     5281 gatcgaagtg atgaaactgg tcggtgccag cgacgggttt gcccgacggc cgtttctcta
     5341 tagcggcatc tggtacgggc tcggcggcgg gctcatggcc ttgacgctgg tggcgattgc
     5401 agtggcggtg ctggcgaggc cggtcgatga gctggcaaag ctctacggca gcccgttcca
     5461 ccttcaggga ctctccctcc cccttggggc gagcgtgctt gcgctggcgg ttggcttatc
     5521 gtggtttggc tcctggcttg ctgcgacgcg gcatattcgc gggatcgacc ccagctaaac
     5581 ccgatgctta tcaacgcctt aacgagaatg tgaactatcg gtaacatcag cactccaacc
     5641 ctaggagtgc tactatggcg ctcgcatgga tcgggaggtc tgatgactac tgctttagtc
     5701 gctaaacaag ctggtgggtt ggtgtttgcc ggacccgtcg gcagcttgga ctcttacatt
     5761 gaccgcgtat cccaaattcc ggttctatcg aaggaagacg aagtcgcgct ggcgactcgc
     5821 tttcgctccg aggccgattt ggatgccgca cggcaactgg tgctgtcgca cctgcgattc
     5881 gtggtgcaca tcgcccgcgg atacttgggc tatggcctgc cgatgggcga tctcgtccaa
     5941 gaaggcaatg tcggactcat gaaggcggtc aagcgcttcg accccaccgt gggcgtgcgc
     6001 ctggtgtcct ttgccgttca ttggatccgc gccgagattc acgagtatgt gctgcgcaac
     6061 tggcgtctgg tcaaggtcgc caccaccaaa tctcagcgca aactcttctt caatctgcgc
     6121 aagatgaaga aaaatctggc gtggctgtcc gaagccgaga ccgccgcggt ggctcgcgat
     6181 ctgggcgtcg acgtcgccga cgttcgcgaa atggagcaac gtctgtccgc gcgcgacatg
     6241 tccttcgatc cgacaccgga aagcgacgag gaagaagcct actctccggc catgtacttg
     6301 ccgtcgagca atgccgatcc ggcgatcgag gtcgaaaacg aggagtggga agaggactcg
     6361 accgagcgcc tgagcctcgc actcgagaag ttggatgagc gcagccgcag catcttgaag
     6421 cggcgctgga tgacggacga caaagccacg ttgcatgagc tcgcggatga atacggaatt
     6481 tccgcggagc gcgtgcggca ggtcgagtcg aatgccatca gcaaactcaa agggctgatg
     6541 gcagcggcct aaagctcaat gtcggcgccg ctcggtgtca tcgaggggtt cttcggaaag
     6601 ccctggaccg acaccgcgcg tctcgcctgc gctgaattcc tgcggccgcg cggctttgat
     6661 ttctacgtct gcgccccgaa gtccgatcgg ttcttgcgcc gccaatgggc cgaccctttc
     6721 ccggcgcccg cactcgcgca gctctgcgag ctgtcctcgc aaatacgaga gcgggcggtc
     6781 cgattcggca taggcctgtg tccgtacgac attcaccgta actacgatgc ggcggcccgc
     6841 gcggcgctgc gtgccaaggt ggcgcagctc gacgaaatcg gcaccgagct gctttgcatc
     6901 ttgttcgacg atatgccggg cagctttgcc gggctcgcac cactgcaggc gcgagtcacg
     6961 gccgatatca cggagtggag ctcggccgcg gctttcatcg tgtgtccgac ctactactcg
     7021 gacgatccga tcttggcacg cgtcttcggt cccgccccgc aaaactatct gcaagatctc
     7081 ggccgcggac tcgatcccgc ggtcgatgtg ttttggacgg gacctgaggt gtgttcgtcg
     7141 ggatatccgg acgagcacct ggaggacgtc gcgacccggc tcggccgcag acccttcatc
     7201 tgggacaacc acatcgccaa tgacggcaag ctgcgctgct cgcacgtgta cctcgacccg
     7261 cattcctcgg gctggtcgct caaccgtcgc ctggctgccg gccttgcggt caatcccatg
     7321 aatcagccgg gcctgtcgcg gattgcgctg gccagctacg cggccggcgc tgccgacgcg
     7381 gcgctcgagt cgcacgttcg ctcggtgtgc agcgcgccgc tcggcgccgc gttgctgggc
     7441 gatgtcggtc tgtttcaagg tcgcggcatc gccgcgctcg acgccgccgg ccgctcggcg
     7501 ctcgccgaga agtatcgtcg cttcgagccg caatggtgtg cgcgtgaaat catcgcctgg
     7561 ctcgaaggtg aatacgaatt cgatccgaat tgtctgacac aatgagcgtc tatggcgcgt
     7621 cgtgtcgctc cggtgtggtt gatgggactg acgaacgccg tgttcggaat gtacggcggc
     7681 attctggtga tttcagtgcc gcaactgctg agcgcgcagc atgtgcccga agcgaccatc
     7741 gcatcgatga ccgcggtcat gctgtcgccc ggcttctgga catttctcgt gagcccggtg
     7801 ttggacgtgc gcttcagccg ccgctggtat tcggcattca ccgccatggt cgccgcggcg
     7861 ctgctggttg tggcgcttct caacctgaga aatctcgcat tggcggaaat actcctcgtg
     7921 acgggttatt tcttcgccaa tctgtaccag agtgcgctcg gcggctggct cgcaagcatc
     7981 acgaccaagg aagaggaaag caagctcagt gtctgggtga ccgtcggcaa tatcggcacc
     8041 ggcggcgcaa tggcattgat gaccggcgag atcgtgcgca atttgccgcc gctcaccgcc
     8101 gccttgctgc tcggcgcctt gctgctgctg ccgaccgcgg tttttccatg gatgcctgcg
     8161 ccaggtccgg accggcgtct ggcgcgcgag agtttcccgc agtttttcgg cgaagtggtc
     8221 agcctcttga agcagcgcga agtattgatc gcgattctgc tgttcatcgc gccggcggcc
     8281 accttctcgc tgacgaactt tctcggcggc ctgggcgctg acttccacgc atcgacccat
     8341 gtcgtagggt
//
LOCUS       DASWZT010000117         6834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_83060, whole genome shotgun sequence.
ACCESSION   DASWZT010000117 DASWZT010000000
VERSION     DASWZT010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83060"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..109)
                     /locus_tag="VGE92_03185"
     CDS             complement(<1..109)
                     /locus_tag="VGE92_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY1312849.1"
                     /translation="MRHLIAILLQNEAGALTRVTGLISTRGYNIESLSVA"
     gene            231..2009
                     /gene="ilvB"
                     /locus_tag="VGE92_03190"
     CDS             231..2009
                     /gene="ilvB"
                     /locus_tag="VGE92_03190"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEY1312850.1"
                     /translation="MTGADMLVQVLADEGVDTIFGYSGGAILPIYDAVFRYNLEHQSS
                     MPLIVPANEQGAGFMAAGYARASGRVGVALVTSGPGATNTVTPVRDSMADSVPIVVIC
                     GQVPTTAIGTDAFQEAPVPSIMGAVAKHVFLVTDPSKLEATVRTAFEIARTGRPGPVV
                     LDVPKDVQNWKGVFHGSGRLPVPGYRQRMNRLEASKLPEAACADLFAALGAAKRPLIY
                     AGGGVINGSAAHALREFCKEFQIPVVTTLMGIGAVDTTHPLSMRMLGMHGAAFANYAV
                     DDCDCLLALGARFDDRVAGNPTKFAGAAKFIAHFDIDASEINKVKRVDWSHVGLMPDA
                     LLVLLDYGKRKGFKRDWSEWHRHCAQLRAKYAMNYDRDSTLIQPYYVIEEINRLTRGE
                     AIITTGVGQHQMWAAQYFDFRTPRLWLTSGSMGTMGFGLPAAVGAQYAQRNRLVIDID
                     GDASIRMNIGELETVTTYDLPIKVVVLNNCGDGMVKQWQKLFHAGRLSASDKSLHTKD
                     FVKAALADGFKYAARLDKKADVPRIIDQFIRFEGAAFLEVMIDPDAGVYPMVGPGQAY
                     AEMITGEHIPSRTLVDIKTPDASEMF"
     gene            complement(2019..2438)
                     /locus_tag="VGE92_03195"
     CDS             complement(2019..2438)
                     /locus_tag="VGE92_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY1312851.1"
                     /translation="MKYLHTMVRVTDIEQSLLFYRDALGLKELSRRDFPQGRYTLVFL
                     AAAGDEDAQVELTYNWDPQAYPGGRNFGHLAYAVDDVYATCRRLMEHGVTINRPPRDG
                     RMAFVRSPDDISIELLQKGAALAPAEPWTTMPNSGVW"
     gene            2486..2866
                     /locus_tag="VGE92_03200"
     CDS             2486..2866
                     /locus_tag="VGE92_03200"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY1312852.1"
                     /translation="MHKSKLSGFIIDCQTDDLHQAAAFWGAALGMPVRELPAEEAALY
                     KRLEDSVHGLDIEVQKVEHASRVHLDIETDDIDAEVKRLEKLGAKRIQAVRTWWVMEA
                     PTGHRFCVVHAASAAFARQSTEWP"
     gene            3291..3914
                     /locus_tag="VGE92_03205"
     CDS             3291..3914
                     /locus_tag="VGE92_03205"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY1312853.1"
                     /translation="MQSGATSVGSRGASSGTAPLGLQLRPLEELVRAMAAGDRGALSS
                     VYEQTVAQVFAIARSMLRSKEDAEEVVCDVYTHAWQRAGSYDASRGTVMAWLAVMARN
                     RAIDRLRQRRESLSLDDDQHGALAASLVGEVLGPEQLLAQFQSGSAVHRALRSLTAQR
                     RHLLGLAFFEGLSHQEIACVTGMPLGTVKSHLRRALAALQDELTLDA"
     gene            3953..4405
                     /locus_tag="VGE92_03210"
     CDS             3953..4405
                     /locus_tag="VGE92_03210"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY1312854.1"
                     /translation="MLDPTAVQHLAAAILPAELPAADRDAMHSRIMQRISDAAPAGTV
                     TFRAADMHWVTAGPGVEVKVLRMDRERNDQTVLIRMHPGSVVVGHRHTQEEECLVLEG
                     EIFIGNYRLGQGDMHVARPGVVHAPIHSPNGALLMIRSEMPPRSFRIA"
     gene            4415..5671
                     /locus_tag="VGE92_03215"
     CDS             4415..5671
                     /locus_tag="VGE92_03215"
                     /inference="COORDINATES: protein motif:HMM:NF009587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEY1312855.1"
                     /translation="MKTALRSLYFQVIVAIVLGVLLGTLYPAVGAAMQPLGDGFIKLI
                     KMLIAPVIFCTVVTGIAGVENLKQVGRTGGLALIYFEVMSTVALTLGLLVMNILRPGR
                     GMNVDARSLDAASVAVYADPKKLQSVSDFLLNIIPTTVVDAFAKGEILQVLLFAVLFG
                     FALQRLGQRGVPLLGFIDALSQVLFGIVGVVMRLAPLGAFGAMAFTIGKYGFGSLLSL
                     GLLMGCFYATCVLFIFLGLGAVARLHGFGIWQFVRYIRDELLIVLGTSSSESVLPRMI
                     AKLQRAGASKSVVGLVIPAGYSFNLDGTSIYLTMATLFIAQATNTPMNLLQQLTLLAV
                     LLLTSKGAAGVTGSGFIVLAATLSSVGTLPVAGLALILGIDRFMSEARALTNVIGNGV
                     ATLVVAKWTGELDMVTMRRELGGRTI"
     gene            5671..6657
                     /locus_tag="VGE92_03220"
     CDS             5671..6657
                     /locus_tag="VGE92_03220"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF007989.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEY1312856.1"
                     /translation="MRISDGFTDAIGNTPLIRLRHLSDETGCEILGKAEFMNPGGSVK
                     DRAAKWIVLDAERRGLLKPGGVVVEGTAGNTGIGLAHVCNARGYRCVIVMPDNQAAEK
                     YQIIEALGAELRKVPAVPYANPNQYQKVAGRLAAELPDAVWANQFDNTANRDAHFEST
                     GPEIWRDTDGKIDAFCASTGTGGTLAGVARYLKSKSSAVRIVLVDPPGSSLYHYIKDG
                     ELKSDGGSSITEGIGTGRVTANLEGTPIDDALRIADAETMHFVYRLLREEGLLLGSTA
                     GINVAAAVTLAKQLGPGHTIVTILCDGGSKYQSRLFNPAWIAERGFVEAIAR"
BASE COUNT         1288 a         2018 c         2201 g         1327 t
ORIGIN      
        1 gtgccaccga caaggactcg atattgtaac cgcgagtcga tatcaggccg gtgacgcgcg
       61 tcagcgcacc cgcttcgttc tgcaggagta tggcgatgag atggcgcatt ggccggcaag
      121 catacgggag ggtggtactt gggcgccagg gcgagaactg ctagtctttc ccggtgaaag
      181 accgttacga catgttaaat caggccgctc atcccctggc aggcgccagt atgacgggcg
      241 cggatatgct ggtgcaggtg ctggccgacg aaggcgtcga cacgattttc ggctatagcg
      301 gcggcgccat cctgccgata tacgacgccg tattccgcta caacctcgag caccagtcct
      361 cgatgccgct gatcgtgccg gccaacgagc agggtgcggg gttcatggca gcgggctacg
      421 cgcgcgccag cggcagggtt ggcgtggccc tggtcacgtc gggtccaggt gccaccaaca
      481 ccgtaacgcc ggtgcgcgat tcgatggccg attcggtgcc catcgtggtc atctgcgggc
      541 aggtaccaac gaccgcgatc ggcacggacg cgtttcaaga ggcacccgtg ccgagcatca
      601 tgggcgcggt cgcgaagcat gtattcctgg ttaccgatcc cagcaaactg gaagcaaccg
      661 tgcgcacggc gttcgaaatc gcgcgcaccg ggcgtccggg ccccgtggtg ctcgatgtgc
      721 cgaaagacgt ccagaactgg aagggtgtgt ttcacggctc gggtcggttg ccggtgcccg
      781 gctatcggca gcgcatgaat cgcctcgagg ccagcaagct accggaagcg gcgtgcgccg
      841 acttgttcgc cgctctcggc gccgctaaac ggccattgat ctatgcgggc ggcggcgtca
      901 tcaacggcag cgccgcgcat gcgctgcgcg agttttgcaa ggaatttcag attcccgtcg
      961 tcacgacgct gatgggcatc ggcgccgtcg atacgacgca cccgttgtcg atgcgcatgc
     1021 tcggcatgca cggtgcggcg ttcgccaact acgcggtcga cgactgcgac tgtctgctgg
     1081 cgctcggcgc gcgtttcgac gatcgggtcg ccggcaaccc gaccaagttc gcgggcgccg
     1141 cgaaattcat cgcgcatttc gacatcgatg cctcggaaat aaacaaggtc aagcgggtgg
     1201 actggagcca tgtcggattg atgccggatg cgctgctggt gctcctggat tacggcaagc
     1261 gcaagggctt caaacgcgac tggtccgagt ggcaccggca ttgcgcccag ctgcgcgcga
     1321 aatatgccat gaactacgat cgcgacagca cgctgatcca gccttactac gtgatcgaag
     1381 aaatcaatcg gctgacacgc ggcgaggcca tcatcaccac cggcgtcgga cagcatcaaa
     1441 tgtgggccgc gcagtacttc gatttccgta cgccgcggct ctggttgacg tccggcagca
     1501 tgggcaccat ggggttcggt ctgccggcgg cggtgggtgc gcagtatgcg cagcgcaatc
     1561 gcctggtcat cgatatcgac ggcgacgcca gcattcgcat gaacatcggc gagctcgaaa
     1621 cggtcacgac ctacgatctg cccatcaaag tcgtggtgct gaataattgc ggcgacggca
     1681 tggtcaagca gtggcagaag ctgttccacg ccggccgttt gagcgccagc gataaaagtt
     1741 tgcataccaa ggatttcgtc aaagcggcac tggcggatgg cttcaagtac gcggcgcgtc
     1801 tcgacaagaa ggcagacgta ccgcgcatca tcgaccagtt catccgcttc gaaggcgcgg
     1861 cattcctcga ggtaatgatc gatccggacg ccggcgtcta tcccatggtg gggccgggcc
     1921 aggcatatgc cgagatgatc acgggcgagc acattccatc gcgcactttg gtcgacatca
     1981 agacgccgga tgcgtcggaa atgttttgat tcccgcggct accacacgcc gctgttgggc
     2041 atcgtggtcc aaggttccgc cggtgccagc gctgcgccct tctgcagcag ttcaatcgaa
     2101 atgtcgtccg gcgagcgcac gaaagccatg cggccgtcgc gcggcggccg gttgatcgtg
     2161 acgccgtgtt ccatcaagcg ccgacacgtg gcgtagacat catccacggc ataagccaga
     2221 tgcccgaagt tgcggccgcc cggatacgcc tgcggatccc aattgtaagt gagctcgacc
     2281 tgcgcatcct cgtcgccggc ggcggccagg aaaaccaatg tgtagcgccc ttgcgggaag
     2341 tcgcgccgcg atagctcttt caatccgagc gcgtcgcgat agaagagcaa cgattgctcg
     2401 atatccgtca cgcgtaccat agtgtgtaaa tatttcatgg acagtgcgat tgaatttttg
     2461 cggtatttgg aatttggagt aagcgatgca taaaagcaaa ctttcgggat tcattatcga
     2521 ttgccaaacc gacgatttgc accaagccgc cgccttttgg ggcgccgcgt tgggaatgcc
     2581 ggtgcgcgaa ctgcccgccg aggaggccgc actttacaaa cggctcgaag attcagtgca
     2641 cggcctggac atcgaggtgc aaaaggtcga gcacgccagc cgcgtgcatc tcgacatcga
     2701 aaccgacgac atcgacgcgg aggtcaagcg cctcgaaaaa ttgggcgcaa agcgcattca
     2761 ggcggtacgc acgtggtggg tcatggaggc gccgacggga caccgtttct gtgtggtgca
     2821 cgccgcatcg gcggcgttcg cccgccagtc caccgagtgg ccgtagtgcg ccggcggccc
     2881 ggaagagtgt cttctgtcac aatttggcga cacacttgac atagtgcagc aacattcaag
     2941 catagcgcca taactactag atttgcctac gatatctgtt attattgggt cgtcgactca
     3001 gcgcgcattt gaaaccagcg ttacttagtt gcaacactgg aagagacaaa caaggtcgaa
     3061 cgtaagggct tttatgcccg gtacgtatct tccattctag gacgtgtcgg gtttttaccc
     3121 gacacctgtt ccactcgaat tccccggcct gtccggggat tcttttgccg gcgaccctaa
     3181 gcgctgcatc cgagttaccc atcgttgcgt aaatctaggc actgtccaat tgaatccaat
     3241 tgaggagccg gcgcgaatgg ccttggtaac caatcaaggt cgagggtcga atgcagtcgg
     3301 gggccaccag tgtcggcagc aggggcgcga gcagcggcac ggcgccgtta gggcttcaat
     3361 tacgacccct cgaagagctg gtgcgcgcca tggcggcggg tgatcgcggc gcgctgtcga
     3421 gcgtttacga gcagaccgtg gctcaagttt tcgccattgc gcgcagcatg ctgcgctcca
     3481 aggaagacgc cgaggaagtc gtgtgcgacg tttacaccca tgcttggcaa cgcgcgggca
     3541 gctacgacgc gagtcgcggc acggtcatgg cctggctcgc cgtaatggcg aggaaccgcg
     3601 cgatcgatcg cctgcgccag cggcgcgaat ctctgtcctt ggacgatgac caacacggtg
     3661 cgcttgccgc atcgctggtc ggcgaagtgc tcgggcccga gcagctgctg gcgcaattcc
     3721 aatccggcag cgcggtgcat cgcgcattgc gatcgctgac tgcgcagcgc cgccatttgt
     3781 tggggttggc tttctttgaa gggctgagtc atcaggagat tgcctgcgtc accgggatgc
     3841 cgttgggtac ggtaaagtcg catctgcgcc gcgcgctcgc ggccttgcaa gacgagctca
     3901 cactcgacgc gtaatgcgca agcgttccaa acagccaaag ccagcagccg acgtgctgga
     3961 ccccacggcc gtacaacatc tggcggccgc gattctgccg gccgaattgc ctgcggcgga
     4021 tcgcgacgcc atgcacagcc gcatcatgca gcgtatcagc gacgctgcgc cggcgggtac
     4081 ggtgacgttt cgcgccgccg atatgcactg ggtgacggcg ggtcccggag tggaggtcaa
     4141 agtgctgcgc atggatcgcg agcgcaacga ccaaactgta ttgattcgca tgcaccccgg
     4201 atcggtggtg gtcggtcatc gccacaccca ggaagaagag tgcttggtgc tcgagggcga
     4261 gatcttcatc ggcaactatc gtctcggaca aggcgacatg cacgtcgccc gcccgggggt
     4321 ggtgcacgcg ccaattcatt cgcccaacgg ggcgctgctg atgattcgct ccgaaatgcc
     4381 accgcggagt ttccgaatcg cttaaatttg gcgggtgaag accgcgctgc gctcgctgta
     4441 ttttcaggtc attgtcgcca ttgttctggg cgtgctgctg ggtacgttgt acccggctgt
     4501 aggcgccgcg atgcagcccc tgggcgacgg attcatcaag ctcatcaaaa tgttgatcgc
     4561 gccggtgatt ttctgcacgg tggtgacggg aatcgccggc gtcgagaacc tgaagcaggt
     4621 cggaagaacc ggcggcctgg cgctgattta cttcgaagtc atgagcacgg tggcgctcac
     4681 cctgggcctg ttggtgatga atatcctgcg cccggggcgc ggcatgaacg tcgatgcccg
     4741 cagtcttgat gcggcttcgg tcgcggtgta tgccgatccg aagaagctac aatcggtgag
     4801 cgactttctg ctcaacatca tcccgaccac ggtcgtggat gctttcgcca aaggcgaaat
     4861 cctgcaggtg ttgctgtttg cggtgctgtt cggtttcgcg ctgcagcgcc tcgggcagcg
     4921 tggcgtgccg ctgctgggtt tcatcgatgc cctgtcgcag gtgttgttcg gcatcgtcgg
     4981 cgtggtcatg agattggcgc cgctgggcgc attcggcgca atggccttca ccatcggcaa
     5041 gtacggcttc ggatcgctgc tgtcgctcgg cctattgatg ggctgcttct atgcgacctg
     5101 cgtgctgttc atttttctcg gcctgggtgc ggttgcgcgt ctgcacggct tcggcatttg
     5161 gcagttcgtc cgctatatcc gcgacgaact gctcatcgtc ctagggacgt cgtcctccga
     5221 atccgtcctg ccgcgaatga tcgcgaagct gcaacgggcg ggcgccagca aatcggtggt
     5281 cggactggtg attccggccg gctattcgtt caatctcgac ggcacatcga tttacctcac
     5341 gatggcgact cttttcatcg cgcaggccac caacacgccg atgaacttgc tgcagcaact
     5401 cacgctgctc gccgtgctgc tgttgacctc gaagggtgcg gccggcgtca cgggcagcgg
     5461 cttcatcgtg ctcgccgcga cgctgtcctc ggtgggtacg ctgccggtcg ccggcctggc
     5521 cttgatcctc ggtatcgatc gattcatgtc ggaggcccgc gcactgacca atgtgatagg
     5581 aaacggcgtc gcgacactgg tcgtcgcgaa atggaccggc gagttggata tggtcacaat
     5641 gcggcgtgaa ctcggtggaa ggacaatttg atgcgcataa gtgacggctt tacggacgcg
     5701 atcggcaata ctcccttgat tcggctgcgt catttgagcg acgaaaccgg ctgcgagatc
     5761 ctgggaaagg cggaattcat gaatcctggg ggctcggtga aggatcgcgc cgccaaatgg
     5821 atcgttttgg acgccgaacg ccgcggactg ctcaaacccg gcggtgtcgt ggtcgagggg
     5881 acggcgggca ataccggcat tggcctggcg cacgtgtgca acgctcgcgg ctatcgctgc
     5941 gtgatcgtaa tgcccgacaa tcaggctgcc gagaaatacc agatcatcga ggcattgggg
     6001 gcggagctgc gcaaagtgcc ggccgtgcct tacgccaatc cgaaccaata tcagaaggtg
     6061 gcggggcgcc tcgcagcaga gctgcccgat gccgtgtggg ccaaccaatt cgacaacacg
     6121 gcgaatcgcg acgcgcattt cgagtcgacg ggccctgaga tctggcgcga caccgacggg
     6181 aaaatcgatg ccttctgcgc gtccaccggc accggcggga cgttggcggg cgtcgcccgc
     6241 tatcttaagt cgaagtcgag cgcggttcga atcgtgctgg tagatcctcc cggcagctcc
     6301 ctgtaccact acatcaagga tggcgagctg aaatccgacg ggggctcgtc gatcaccgaa
     6361 ggtatcggca ccggccgggt gacggcgaat ctcgagggta cgccgatcga cgatgcgctg
     6421 cgaatcgccg atgccgagac catgcacttc gtgtatcggc tgctgcgcga ggaaggcctg
     6481 ctgctcggga gtaccgccgg gatcaacgtg gcggcggcag ttacgctcgc gaaacaactc
     6541 ggcccgggac acaccatcgt gacgattctg tgcgacggcg gatcgaagta tcagtcgagg
     6601 ctgttcaacc ccgcgtggat cgcggagcgc ggctttgtcg aggcgatcgc ccggtaggcg
     6661 atcgcgcggc ggttctgcta tgcggtgagc gacagatcga cgccggccgc ggcgttgaac
     6721 caggccagcg tcgcgtgcaa ttcgaccact ttgccgacga cgagcagcgc cggcgactca
     6781 agttttgcat cagcaacagc ggcgccgagt gtggcgagcg tcgcggttac gacg
//
LOCUS       DASWZT010000118         3315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_20235, whole genome shotgun sequence.
ACCESSION   DASWZT010000118 DASWZT010000000
VERSION     DASWZT010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20235"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VGE92_03225"
     CDS             <1..187
                     /locus_tag="VGE92_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312857.1"
                     /translation="GERGESAAHQGKACGQYATSYCSKNPHVGGIVNRETRLRTNGIV
                     IVARAPSGSVVGAVLTP"
     gene            258..431
                     /locus_tag="VGE92_03230"
     CDS             258..431
                     /locus_tag="VGE92_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312858.1"
                     /translation="MTNTQQVLRGSFLMGLIALASACVVAEPREGYYDHDHHRYWQEH
                     TWHECGDHDDRCR"
     gene            573..3227
                     /locus_tag="VGE92_03235"
     CDS             573..3227
                     /locus_tag="VGE92_03235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY1312859.1"
                     /translation="MTQESLASASSFRIPLLVAACAAVVLALGLFAYRSLAILSDGTA
                     VAAAAERGHFQIAQVLQTLTVMGGGVRHFESTGDTDSFEEATAAAKLLPGQLDALQLA
                     LADDPSQRALIAQLMVLAQQRQAQAQQLIDRALSGDAAGVEALVTRGEGTRIIEDCRA
                     LIARMQDRQTRFAAARAALASRARSTVGFAIGATTVLAMLLLVLVAWGTTRHNARLRT
                     SQEDLSTTLRSIGDAVISTDASGNIRFMNVVAEHLTGWTTANARGLALEQVFRIIHEG
                     TREAAENPVSRVLRERSIVGLANHTVLVGCAGQERPIEDSAAPIYGPAGDVTGVVLVF
                     RDATDQRAAQRALLASEANLREANASLESRIAERTAQLLAREVLIGTFYQHSSECHAV
                     LSQTGAGAFRYEEVNPATLRLYGQTREQVIGHTIDEIFEAPTAAALNHHLAACLRGDG
                     PYRYERSHGEAVIEAIATPVPHEAGVGRRIVVSARDVTERRRLELQLMQSQKMEAVGQ
                     LTGGLAHDFNNLLAGITGALDLIKSRVAQGRLNDLDRYIAAGQGAARRAAALTHRLLA
                     FSRRQTLDPKPTDVNRLVGDMVELIRRTVGPEIKVDIVGAIGLWNPSVDANQLENALL
                     NLCINARDAMPAGGKITIETANRWIDERAAKERDLAPGQYISLCVSDTGCGMSSEVMA
                     RAFDPFFTTKPLGQGTGLGLSMIYGFARQSGGQVRIYSEVGQGAMVCIYLPRHPGHEL
                     PAEPLADPGAIAPSASGETVLVVDDEPLVRMLVTEVLQDLGYRAIECEDGVAALKVLE
                     SSRRVDLMVTDVGLPNGINGRQLADAGRAIRPSLKVLFITGYAENAVFSHGHLNPGFE
                     VLTKPFAMDVLANRISGMMSRAAPPTSE"
BASE COUNT          639 a          984 c         1069 g          623 t
ORIGIN      
        1 cggcgaacgc ggcgagtcgg ccgcccatca aggcaaggcc tgcggtcaat acgctacaag
       61 ttattgctct aaaaatcccc acgtcggcgg cattgtaaac cgggagacac ggttgcgcac
      121 caacggaatt gtaattgttg cacgcgcgcc gtccggttcg gttgtcggcg ccgtgctgac
      181 accatgagtt ggaagttccg gacagcgcgc cgcgccgtcc agggttacgc ttcgcgagca
      241 atcacgcagg agcaatcatg acgaacacac agcaagtatt gaggggcagc ttcttgatgg
      301 gactgatcgc gctggcatcg gcttgcgtcg tcgccgagcc ccgcgagggc tattacgacc
      361 acgatcatca ccgctattgg caggagcata cctggcacga gtgcggcgat cacgacgatc
      421 gttgccgctg atcggctacg agtggggcgc gattgtcgca actgggcaat cgcgctgact
      481 ggcaggtatt tccataacat tcccgcgagc gggcgccgat agtatgtata tttaggcgcc
      541 gcgtgcttga cgcggcaaaa gattctcaag ttatgacgca ggaatccttg gcgtcggcct
      601 cgtcatttcg tatcccgctg ctcgttgccg cctgcgctgc cgtggtgctg gcgctgggat
      661 tattcgctta ccgaagcctc gcaatattaa gtgacgggac cgcggtggcc gcggctgccg
      721 agcgtggcca ctttcagatt gcccaagtgc tgcaaacctt gaccgtgatg ggcggcggcg
      781 tgcgccactt cgaatcgacc ggcgacacag attccttcga agaagcgacg gcggcggcga
      841 aattactgcc gggccagctc gatgccttgc aactcgccct ggcagatgat ccctcgcagc
      901 gtgctctcat cgcacagctc atggtactgg cgcagcagag gcaagcccag gcgcagcaat
      961 tgatcgaccg agcgctgagc ggcgacgccg caggcgtcga agctttggtc acccgggggg
     1021 aggggactcg aatcatcgaa gactgtcggg cgctgatcgc caggatgcag gatcgacaga
     1081 cgagattcgc ggcagcgcgc gcggcgctgg catcgcgggc gcgcagcacc gtgggattcg
     1141 cgatcggtgc gacgaccgta ttggcgatgc tgctgctggt actcgttgcc tggggcacca
     1201 cgcgccataa cgcgcgactg cgcacgagcc aggaggacct gtcgacgact ttgcgcagta
     1261 tcggcgacgc cgtaatttcg acggatgctt ccgggaacat ccgattcatg aatgtcgttg
     1321 ccgaacactt gaccggttgg accaccgcga atgctcgcgg cttggccctg gagcaggtgt
     1381 ttcgcatcat ccacgaaggc acgcgcgaag cggccgaaaa tcccgtaagc cgcgtactgc
     1441 gcgagcgcag catcgtcggc ctcgcgaacc acacggttct cgtaggttgc gcgggacagg
     1501 agcggccgat cgaggattct gccgcgccca tctatggacc cgctggcgat gtgacgggcg
     1561 tggtgctggt gtttcgcgac gcgacggatc agcgcgccgc gcagcgcgcc ttgctcgcca
     1621 gcgaggcgaa cctgcgcgag gcgaacgcca gtctggaaag tcgaatcgcc gaacgcacgg
     1681 cgcagttgtt ggcgcgggaa gtgctgatcg gcactttcta tcagcattcc tcggaatgtc
     1741 acgcggtgct cagccaaact ggtgcgggtg cgtttcgtta cgaagaggtt aatccagcca
     1801 ccttgcgtct gtatggccaa actcgcgaac aggtcatcgg acacaccatc gatgagattt
     1861 tcgaggcgcc gacggcggcc gcgctcaatc accatcttgc tgcatgcctg cgcggcgatg
     1921 gcccttatcg ctacgaacga tctcacgggg aggcggtcat cgaagcgatt gcaactccgg
     1981 tcccgcacga ggcgggtgtc ggccgtcgca tcgtcgtcag cgcgcgcgat gtgaccgagc
     2041 gccggcggtt ggagctgcaa ttgatgcaat cgcagaagat ggaagcagtc gggcaactga
     2101 ccggcggcct cgcccatgac ttcaacaatt tactggcggg catcaccggc gcgctcgatt
     2161 tgatcaaatc gcgggttgca caggggcgat tgaacgacct cgatcgttat atagcggcgg
     2221 gtcaaggcgc ggcgcggcgc gccgccgctt tgacgcatcg cctgctcgcg ttctcgcgac
     2281 gccagactct cgatccgaaa ccgaccgacg tcaaccgctt ggtcggcgac atggtggaac
     2341 tgatcagacg cacggtaggg ccggaaatca aagtcgacat tgtcggtgcc atcgggctct
     2401 ggaacccgtc ggtcgatgcc aaccagctgg agaacgcgct gctcaatcta tgcatcaacg
     2461 ctcgcgatgc gatgcccgcc ggcggcaaaa taacgatcga aacggcgaac cgctggatcg
     2521 atgagcgtgc cgccaaggag cgggacctgg caccgggaca atacatttcg ctgtgcgtga
     2581 gcgacaccgg ctgcggaatg tcgagcgaag tgatggcgcg cgcgttcgat ccgtttttca
     2641 cgaccaaacc tctggggcaa ggtaccggcc tgggactgtc gatgatttac ggatttgccc
     2701 ggcaatccgg cggccaagtg cgaatttatt ccgaagtagg tcaaggggcg atggtatgca
     2761 tctatctgcc gcgccacccg ggccatgagc ttccggccga gccgcttgcg gaccccggcg
     2821 cgatcgcgcc gtcggcctcg ggcgagaccg tcttggtcgt cgacgacgaa cccttggtgc
     2881 gcatgttggt cactgaggtt ttgcaggact tgggatatcg cgcgatcgag tgcgaggacg
     2941 gcgttgccgc cttgaaggtg ttggagtcct cgcgtcgcgt cgacttgatg gtcacggatg
     3001 tgggtctgcc gaatggcatc aacggacgcc aattggcgga cgccggccga gcgattcgcc
     3061 cgtcgctcaa ggtattgttc atcacgggct acgcggaaaa tgcagtgttc agccatggac
     3121 atctcaatcc agggttcgaa gtccttacca agccattcgc catggatgta ctggcgaatc
     3181 ggatctccgg catgatgagt cgcgccgcgc cgccgacgtc cgaatgagcg gcggcgttga
     3241 aggcataccc acaacgccaa ttcccttgcg ccgtgattgc aaatcgcggg ttccgaagca
     3301 tatgaagttg aacat
//
LOCUS       DASWZT010000119         2675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_87883, whole genome shotgun sequence.
ACCESSION   DASWZT010000119 DASWZT010000000
VERSION     DASWZT010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87883"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="VGE92_03240"
     CDS             complement(<1..808)
                     /locus_tag="VGE92_03240"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY1312860.1"
                     /translation="MASGTSRRTILKTAALASAAVITAPYVRGAYAAGSLALGCWDHW
                     VPGANKALIKLCDEWGAKNKVDVHIDFITSQGEKDKLTAAAEAQAGTGHDIMSHRDWN
                     IRIHQNVLEPLDDVVSALIKKYGAISPVAEYLAKSKGVWLGVPTAIGSQVKPCCSRLD
                     LYKEHAGLDLKDMFPADESKYDKAKTDKWTWDLYLESAQKLHKAGFPVGLPMGQTSDA
                     VDWVGGLFNAYGAVFIDAKDNIKVNSDETRAALEYMKKLMAVNPPEVYAWD"
     gene            1048..>2675
                     /locus_tag="VGE92_03245"
     CDS             1048..>2675
                     /locus_tag="VGE92_03245"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF002550.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEY1312861.1"
                     /translation="MASTQHSETFDYTIVGAGSAGCVLANRLTASGRHRVLLLEAGGH
                     DRNIWIHIPLGYGKLFSNAKVNWLYATEPEPDLNNRKIIQPRGKVLGGSSSINGLLYV
                     RGQPEDYDHWRQLGNAGWSFEDVLPYFRRAEDQERGADELHGAGGPLAVSNVSEPHPL
                     CEAFIGAAQQAGFPRNDDFNGPNQEGAGYFQLTARNGRRCSTAVGYLHPARRRPNLKV
                     ETGALATRILFSGRRATGIEYRQGDTTHIAHANAEVILAGGAFNSPQLLQLSGLGPAE
                     LLRAHDIDVIADMPGVGNDLQDHLQIRMQYRCTEPITMNDVINSWRHRTAAGLRYIVS
                     RKGLLTIGAGYAGAFLRTRPDLATPDVQLHFIIFSADASGATLHPFPGFIASVCQLRP
                     ESRGFVRIKSADPRQPPAIQPRYLSSKFDCDTVVDGLKLLRRIMDQSAMRRYIAEQCA
                     PDPSRTSDDELLAFARESGTTVFHPTSTCRMGSDATAVVDERLRLHGVDGLRVIDASI
                     MPTVVSGNTNAAVVMIAEKGADMILQDAAAHAVM"
BASE COUNT          499 a          848 c          795 g          533 t
ORIGIN      
        1 cgtcccaggc gtaaacctcg ggtggattca ccgccatcag cttcttcatg tattccaacg
       61 cagcgcgggt ctcgtcggag ttgaccttga tgttgtcctt ggcgtcgatg aacacggcgc
      121 cgtaggcgtt gaataatccg ccgacccagt ctaccgcgtc ggaggtctga cccatcggca
      181 gaccgaccgg gaagccggcc ttgtgcagct tctgcgccga ctcaagataa agatcccacg
      241 tccacttgtc ggtcttggcc ttgtcgtatt tcgactcgtc ggccgggaac atgtctttca
      301 gatccagacc ggcgtgctcc ttgtacagat cgagacgtga gcagcatggc ttgacctggc
      361 tgccgatcgc ggtcggtacg ccaagccaaa cgcccttgct cttggcgaga tattcggcga
      421 ccggactgat cgccccgtat ttcttgatga gcgcagagac cacgtcgtcg agcggctcga
      481 gtacgttctg gtgaatgcgg atattccagt cgcgatgcga catgatatcg tggccggtgc
      541 cggcttgcgc ctcggctgcg gccgtgagtt tgtccttctc gccttgcgat gtgatgaagt
      601 cgatgtgaac gtcgactttg ttttttgcgc cccattcatc gcagagtttg atcagcgctt
      661 tgttggcacc gggcacccag tgatcccagc agcccaaggc gagcgagccg gccgcgtaag
      721 caccccgaac atacggggcc gtgatgaccg cagctgacgc gagggctgcg gttttcagta
      781 ttgttcgccg agaagttcct gaagccatcg gggtagtcct ccctgttgac gttgagttat
      841 tgttattttg cagtcaggct atactcaaac cgtatgggtg cctagcggta gcccacaata
      901 tcgcgccgca aaaaccgcta tgaatccagc cgcgccgcac tcggccccca atggcgccgc
      961 gcacgcgtcg ctgctcagaa gtggctatga atcgcatgat ttttgaagtg ggcgggcgcc
     1021 cgcgccaagc gcgaacaagg ccgggccatg gcatcgacac agcattctga gactttcgac
     1081 tacactattg tcggtgcggg ttcagccggc tgcgttcttg ccaatcggct gaccgcttca
     1141 ggccgccatc gcgtgctgct gttggaagcc ggcggccacg atcgcaatat ctggatccac
     1201 attccgctcg gctacggcaa attgttcagc aacgccaagg ttaattggct ctacgccacc
     1261 gaaccggaac ccgacctcaa caaccgcaag atcatccagc cgcgcggcaa agtgctgggc
     1321 ggctccagtt cgatcaacgg cttgctctat gttcggggcc agccggagga ttacgaccac
     1381 tggcggcaac tcggcaacgc cggctggagc ttcgaggacg tgctgcccta cttccggcgt
     1441 gccgaggacc aggagcgcgg cgcggatgaa ctgcacggcg ccggtgggcc gcttgcggtt
     1501 tccaatgtga gcgagcctca cccgctgtgt gaagccttca tcggcgccgc gcaacaagcc
     1561 ggtttcccgc gcaacgacga tttcaacggc ccgaaccagg aaggcgctgg ttatttccag
     1621 cttacagcgc gaaatggacg gcgctgctcg accgctgtcg gctacctgca cccggcgcgg
     1681 cggcggccca atctcaaagt cgagaccggc gcgctggcaa cacgcatttt gttttcaggg
     1741 cgtcgcgcca cgggaattga atatcgtcaa ggcgacacga cacacattgc tcatgcaaac
     1801 gccgaagtga tcctcgccgg cggcgcattc aattcaccgc aattgctgca actctccggc
     1861 ctgggcccgg cggaattgct gcgcgcacat gacatcgacg tgatcgccga catgccaggc
     1921 gtcggcaacg atttgcagga tcatctccaa atccgcatgc aatatcgctg caccgaaccg
     1981 atcaccatga acgacgtcat caatagttgg cgtcaccgca cggccgcggg gttgcgttac
     2041 attgtatcgc gcaagggcct cctgaccatc ggcgctggct atgccggcgc tttcctgcgc
     2101 acgcgtcccg atctcgcgac ccccgacgtg cagttgcact tcatcatctt cagcgccgac
     2161 gcttccggcg cgacgctgca cccgtttccc ggattcatcg cctcggtctg ccagttgcgg
     2221 ccggagagcc gcggcttcgt gcgaatcaaa tcggccgatc cgcggcagcc gccggcgatc
     2281 cagccgagat acctttcgag caagttcgac tgcgacaccg tggttgacgg cttgaagctg
     2341 ctgcgccgga tcatggatca gtcggcgatg cggcgttaca tcgccgaaca atgcgcgccc
     2401 gatcctagcc gcacgagcga cgatgaactt ctggccttcg cgcgcgagag cggaacaacc
     2461 gtctttcacc ccacaagcac ctgccggatg ggctcggacg cgaccgccgt tgtcgacgag
     2521 cggctgcgcc tgcacggcgt cgacgggctg cgcgtcatcg acgcctcgat catgcccacg
     2581 gtggtatccg gcaataccaa tgccgcggtt gtgatgatcg cggaaaaagg cgctgacatg
     2641 atcttgcagg acgcggcggc gcacgcggtt atgag
//
LOCUS       DASWZT010000120         8242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_68611, whole genome shotgun sequence.
ACCESSION   DASWZT010000120 DASWZT010000000
VERSION     DASWZT010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8242
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68611"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..1695)
                     /locus_tag="VGE92_03250"
     CDS             complement(10..1695)
                     /locus_tag="VGE92_03250"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1312862.1"
                     /translation="MSVEAGNRGLAESEAFAGRKAQMFPQLSAAQIARLESHGTRMRV
                     DGGQVLTQPGDRGSPLFVVLAGSIEVVQTGMDGEALVFVHAAGSFTGEMSTLQGNGSL
                     VRARVRDDGEVLAISGEHLRTIVQTDAELSELFMRAFILRRVGLLQSQAGDVILLGSS
                     HSAGTLRIQQFLTRNSFPYVSLDVNTDQSVQGLLERFHVKPEEVPVVLCRGEVVLKNP
                     SNEELAACLGMNQVIDDVRVRDVIIVGAGPAGLAAAVYGASEGLDVLVLETGTPGGQA
                     GSSSKIENYLGFPTGISGLALAARARVQAQKFGAEIRTAYSALKLNCRERPYSIELGA
                     GTKVPARAVIIATGAEYRQLTIENAARFLGTGIYYAATATEVRRCETKDVVVVGGGNS
                     AGQAAVYLAGKCRHVHLLVRAIGLADSMSNYLIRRIDETPNITLHTKTEITALEGDAQ
                     LERVVWKAAPDETLETHTIGHVFLMTGAVPSTRWLRGCIALNDKGFVLTGPDLPAEDA
                     ARWTAARPRQPFETNWPGIFAVGDVRCGSVKRVAAAVGEGSACVQQVHQALHD"
     gene            complement(1692..1964)
                     /locus_tag="VGE92_03255"
     CDS             complement(1692..1964)
                     /locus_tag="VGE92_03255"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEY1312863.1"
                     /translation="MTRNRQQCTHMNQVKAVTPSGAGCQECLAMGDTWVHLRLCRTCG
                     HVGCCDDSKNRHARKHFMHAKHPVITSFEPGESWSWCFIDEIEVSA"
     gene            2102..4582
                     /locus_tag="VGE92_03260"
     CDS             2102..4582
                     /locus_tag="VGE92_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312864.1"
                     /translation="MFSRKYALVPAILLAAAVNAGPAPFDLAGPTLDVTITRGAKTLS
                     ASAVPNLIAGDRVWIKADLPATQSAHYLMVTAFLSGSTNPPPESWFSPCKTWKDKCGH
                     DGLSVTVPDGAQQVLVFLAPETGGDFKTLVNAVRGRPGAFVRTSQDLNQAALDRSRLE
                     RYLAAIRALNDADPARLAEAAPLLARSLAIKVDDKCLDRLPELQAPCLTQGQESLILN
                     DGHSTSIVEALTSGPGSDLAMEASYTPQLSYGYYSPYIASILDIARIFDSFRIAQYQY
                     IPALATQRGDQLALTLNTAPSFHNPQSVLVIALPAVEQSQLPPLHAVNPKDIYCAGRT
                     TLVLPVDGAPLVFSTGYAHDLTLSLTGSDGKSINLPATADPAQGGYVIDTAVLRAAPL
                     GDSVRASLQGYWGFERYHGPSFVLMNAHAKNWELAAGDEDALVVGRQDTVHLQSDSVS
                     CVDAIMLKDPAGKELKAEWKSVRPNELEVKLPLQGAKPGAMTLLVKQYGVADPQPVSI
                     QTFAEAGHFDSFDLHSGDTQGILKGSRLDEVASLAVKNVVFVPGELSTRNGGDELPMI
                     AQDAAAAAGLKPEHSIAAKVTLQDGRVLPLTAGVDAPRPRVTLLSQSVQPSPSSRDSN
                     IQLSNQSELPLDATLTFAVRAQTPAAFTRDETIEVATTDESASAILSFANGGITLENT
                     QVAVSTLNPVKAFGASVFGPLQFRVLAKGIAGDWRPLGNLVRLPVLTGLTCPSTAELA
                     CKLTGANLYLVDSVANDREFTHPVQVPDGFLGTALPVPHPLTGSLFLKLRDDPSVINP
                     TSLDVQQLAPSASDVARSAARQESPSQP"
     gene            complement(4763..5254)
                     /locus_tag="VGE92_03265"
     CDS             complement(4763..5254)
                     /locus_tag="VGE92_03265"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY1312865.1"
                     /translation="MKTLLIVYHSLTGGTLQMARAAARGAQAETALDVSLRAAAEVHA
                     DDVLAASGYIFASPENLAAMSGQMKDFFDRTYYPLLERLNGRPYAVLICAGSDGRSAA
                     QQIERIATGWRLKAIAEPIIVCTHATSTEDILKPKHIPASDLTPCEELGATLAAGMAL
                     GIF"
     gene            5388..5552
                     /locus_tag="VGE92_03270"
     CDS             5388..5552
                     /locus_tag="VGE92_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEY1312866.1"
                     /translation="MLHYAVVFFIIALIAAVFGFGGIAVGAAGIAKILFIVFLIGAVV
                     SFLMSLGRRV"
     gene            5580..5909
                     /locus_tag="VGE92_03275"
     CDS             5580..5909
                     /locus_tag="VGE92_03275"
                     /inference="COORDINATES: protein motif:HMM:NF039524.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312867.1"
                     /translation="MESVIESPTARKISDYANGQVNNFLESVEDLTKALKDIETPDIA
                     RVRAKVKIALVAAKSAVTDTAQQLGSQAKQVGKRTDDFVRDNPWQVIGIAAVVGLAVG
                     ILASRRS"
     gene            complement(5933..7318)
                     /gene="cysS"
                     /locus_tag="VGE92_03280"
     CDS             complement(5933..7318)
                     /gene="cysS"
                     /locus_tag="VGE92_03280"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY1312868.1"
                     /translation="MLKIHNSLSGEKALFKPLRGNEVRMYVCGMTNYDYIHVGHARML
                     TVFDLIQRYLRSLGYNVTYVRNITDIDDKIIERAAANGENWADLARRFTVAMHEDCAT
                     LGLQSPDLEPRATDYIGPIIAMTQTLIDKGYAYVAANGDVMYSVRKFPQYGRLSGKKI
                     DDLRAGSRVQVDDAKLDPLDFVLWKHAKPGEPHWDSPWGQGRPGWHIECSAMSTSLLG
                     GYFDLHGGGEDLKFPHHENEIAQSCAACDTPFVNVWMHNGFVRVNDEKMSKSLGNFFT
                     VREVLKTLRDPEVLRFFLLSSHYRGPINYSSVQLAQADETLLGLYRALKDAGAATVDP
                     AALGEFRRAMDDDFNTPEALAVMQGVARELNQAKAAGNDAAAASAAAALRAMGAVLGV
                     LQQDPAMYLKRSVGEKSIADEEIEMLLAARRSARAAKNFAESDRIREQLTAAGILLED
                     KPGGVTQWRRA"
     gene            complement(7302..>8242)
                     /locus_tag="VGE92_03285"
     CDS             complement(7302..>8242)
                     /locus_tag="VGE92_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEY1312869.1"
                     /translation="LKPTLRFAVPADQIVEFLDVVHGPQRFLSNDIGDFIIRRDDGTS
                     GFFFCNAVDDSLMGVTHVLRGDDHLTNTPRQLMVLDALQMRRPGYGHVGLLVGADGAP
                     LSKRHGSTSALEFRERGFLPAAVLNHLFRLGHASDVDGWLPPSAMPQHFRFEHLGRAP
                     ARFDETQLAHWQKETVERMSSAELAAWLGCGDSSAFIELVRHNVVLPADAVAWRAVVQ
                     GDLPPLGTDEQAVVAAAGPKFFAAAVDAFDQAPSDLKQLTRILKERTGRKGADLYMPL
                     RVALTGQAHGPELAPLLKLLSPEKARHRLESHAQNS"
BASE COUNT         1544 a         2645 c         2454 g         1599 t
ORIGIN      
        1 cgatgtgtct caatcgtgaa gtgcctgatg cacctgctgc acgcacgcgg aaccctcgcc
       61 cacggcggcc gccacgcgtt tgacgctgcc gcaacgtaca tcgccgaccg caaatattcc
      121 gggccaattc gtctcgaaag gttgccgcgg gcgcgcagca gtccaacgcg cggcatcttc
      181 ggcaggcaga tcggggccgg tgagcacgaa ccccttgtcg ttgagcgcaa tgcagccgcg
      241 cagccagcgc gtgctcggca cggccccggt catcaggaac acatggccaa tcgtgtgcgt
      301 ctcgagtgtt tcatccggag ccgccttcca aaccacgcgt tccagttgcg catcgccctc
      361 gagcgctgtg atttcggtct tcgtatgcaa agtgatgttg ggagtctcgt cgatgcgccg
      421 gatgagatag ttggacatgc tatccgcaag tccaatggcg cgaaccaaga gatgcacgtg
      481 gcggcatttc cccgccaggt agactgctgc ttgtcctgcc gaattgccgc cgccgactac
      541 caccacatcc ttggtctcgc agcgcctaac ctcggtcgca gtcgcggcgt aataaatacc
      601 cgtaccaaga aagcgcgccg cattttcgat cgtgagctgg cggtattccg cgccggtcgc
      661 gatgatgacc gcgcgcgcgg gtactttggt gccggcaccg agctcgatgg agtacggccg
      721 ctcgcggcaa ttgagcttga gcgccgagta ggcggtgcgg atttcggcac cgaatttctg
      781 tgcctgcacg cgcgcgcggg ccgcgagcgc gagcccggaa ataccggtcg gaaatcccag
      841 gtaattctct attttggaac tcgaacctgc ctgccctccg ggcgtcccgg tttcgagcac
      901 caacacgtcg agcccttcgc tagcgccgta aacggcagcg gccagcccgg caggtccggc
      961 gccgacgatg atgacgtctc gcacacggac atcgtcgatc acctggttca tccccaaaca
     1021 agccgcgagt tcctcgttgc tgggattctt gagcacgact tcgccgcggc acagcaccac
     1081 cggaacttcc tcgggcttga catgaaaacg ttccaacagc ccttgtacgg actgatccgt
     1141 gttcacatcg aggctcacgt acggaaacga attccgcgtc agaaactgct gtatacgcag
     1201 cgtgcctgcc gaatgactcg agcccagcaa aatcacgtca ccggcctgcg attgcagcaa
     1261 gccgacgcgc ctcaaaatga acgcacgcat gaacagttcg ctcaactcgg catcggtttg
     1321 aacgatggtc cgcagatgct cgccgctgat cgccagtact tcgccgtcgt cccggacgcg
     1381 tgcccgcacc aagctaccgt taccctgcag cgtgctcatc tcaccggtga agctgcccgc
     1441 cgcatgcacg aaaaccaagg cctcaccgtc catccccgtt tgcaccactt cgatgctgcc
     1501 ggcgagcacg acgaacagcg ggctgccgcg gtccccgggt tgggtcaata cctgtccccc
     1561 gtcgacgcgc atgcgagtac cgtgcgattc cagccgcgcg atctgtgcgg cgctcagctg
     1621 cggaaacatc tgcgctttgc gcccggcgaa ggcctcagac tctgccaaac cgcgattgcc
     1681 ggcttccacg ctcatgccga gacttcgatc tcgtctatga aacaccagct ccacgattct
     1741 ccgggttcga acgaggtaat gacggggtgt ttcgcgtgca tgaaatgctt gcgcgcgtgc
     1801 cgattcttcg aatcgtcaca acaccccaca tgcccgcagg ttcggcacag gcgcaaatga
     1861 acccacgtgt cgcccatggc aagacactct tgacaaccgg cgccgctggg cgtcacggct
     1921 ttcacctgat tcatatgcgt gcattgttgc ctatttcttg tcatgttgac tccggtaaaa
     1981 taccgaattg ccgcatcgcg gatcatagcg actgccaccg cttggtgcgc gggagcgaac
     2041 ttcgccggcc gaaggcgttc taagtcgaca ctaaatccgt accagaggcg tcaaagttgc
     2101 catgttttcg cgaaaatacg cgctagttcc cgccatttta ctcgcggcgg ccgtgaacgc
     2161 cggtcctgcg ccattcgacc ttgccggacc caccctggat gtcacgataa cgcgaggcgc
     2221 gaagaccttg tcggcatccg cagtgccgaa tctcatcgcc ggcgatcgcg tctggatcaa
     2281 agccgatctg ccggccaccc aatccgccca ttatctgatg gtgaccgcgt tcttgagcgg
     2341 ctccaccaat cccccgccgg agagctggtt ttcgccgtgc aagacgtgga aagacaagtg
     2401 cggacatgat ggcttgtcgg tcaccgtgcc ggacggtgcg caacaagtat tggtatttct
     2461 ggcccccgaa accggcggtg atttcaaaac cctggtgaac gcggtacggg gccgcccggg
     2521 ggccttcgta cgcacctcgc aggaccttaa tcaagccgca ttggatcgct cgcgtctgga
     2581 acgctatctg gcagcgatcc gtgcactcaa cgatgcggat ccggcgcgcc tcgccgaagc
     2641 cgcgccgctg ctggcgcgca gtctggcgat caaagtggac gataaatgtc tcgacagact
     2701 tcccgaactg caggccccgt gcctcactca gggccaggaa tcgctcatct tgaacgatgg
     2761 ccacagcacc tccatcgtcg aggccctgac ctcgggcccc ggcagtgatt tggccatgga
     2821 agcaagctac acgccgcagc tgagctacgg ctactacagc ccgtacattg cctcgattct
     2881 cgatatcgca cgcatcttcg actctttccg catcgcgcaa tatcagtaca tcccggcgct
     2941 tgccacacag cgcggagacc agctcgcatt gacgctaaat acagcgccgt cctttcacaa
     3001 tccgcagtcc gtactggtga tcgcgctgcc ggccgtggaa cagtcgcaat tgccgccgct
     3061 gcatgcagtc aaccccaaag atatctactg cgcgggacgg accacgctgg tgctgcccgt
     3121 cgatggagca cccctggtgt tctcgacggg ctatgcgcat gacctgacct tgagcctcac
     3181 gggaagcgac ggcaagagca tcaatttgcc cgccacggcc gacccggcgc aaggcggtta
     3241 cgtcatcgac acggcggtgt tgcgcgcggc gcccctcggg gacagcgtgc gcgcctcgct
     3301 gcagggctat tggggcttcg agcgctacca cggcccgagt ttcgtcttga tgaatgcgca
     3361 tgccaagaat tgggagctcg ccgcgggcga cgaagacgcg ctggtggtcg gccgccagga
     3421 tacggttcac ctgcagtccg atagcgtgag ctgtgtcgat gccatcatgc tcaaagaccc
     3481 ggccggcaaa gagttgaagg ccgaatggaa gtccgtgcgc ccgaacgaac tcgaggtgaa
     3541 acttcctctg caaggcgcga aacccggtgc catgactctg ttggtgaagc aatacggcgt
     3601 cgccgatccg caacccgtct ccatccagac ctttgcggag gcgggacatt tcgatagttt
     3661 cgacctgcat tccggcgata cgcagggaat tctcaaaggc agccgcctcg atgaagttgc
     3721 gagtctcgcc gtcaagaatg tggtgttcgt gcccggcgaa ctgtcgaccc gcaacggcgg
     3781 tgacgaactg cccatgatcg cgcaagatgc ggccgccgcg gccggcctca agccggaaca
     3841 cagcatcgcg gccaaagtca cgctgcagga tggacgggtg ttgccgctga ccgccggcgt
     3901 cgatgcgccg cggccgcgtg tgacgttgct cagccagagc gtgcagccct cgccctcgag
     3961 ccgcgacagc aacatccaac tgtccaatca aagcgaattg ccccttgatg cgacccttac
     4021 cttcgcggtt cgcgcgcaga cgccggcggc cttcacgcgt gacgaaacca tcgaagtggc
     4081 gaccaccgat gagtcggcgt cggctatttt gagcttcgcc aacggcggca tcacgctcga
     4141 gaacacccag gttgcggtga gcacgttgaa cccggtgaag gccttcggcg cttcggtctt
     4201 cggtccgctt caattccgcg tgctcgccaa gggaatcgcc ggtgattggc ggcctctcgg
     4261 caatctggtg cgtttgccgg ttcttacggg cctcacctgt ccttcgacgg cggaactcgc
     4321 ctgcaagctc acgggcgcga atctctattt ggtcgattca gtggccaacg accgtgaatt
     4381 cacccatccg gtgcaagttc cggacggctt cttgggaacg gccctgcccg tcccgcatcc
     4441 gctcaccggc agtctgtttc tgaagttgcg cgacgacccc tcggtgatca atccgacctc
     4501 attggatgtg cagcaacttg ccccctcggc gagcgatgtg gcgcgctccg cggcccgtca
     4561 agagtcgccg agccaaccct aggcggcctt tttcctcggc accttggttc ctgcgcggcg
     4621 ggcctccgaa agtccgatgg caatcgcttg ctttctactc gtgaccttct tgccggaacc
     4681 accgctgcgc aacgtccccg cttgcatctc ctgcattgtt ttttcggcaa ttgccgcatg
     4741 cccctgtcgg cggcgcccga cttcaaaata tgccgagagc catcccggcg gccaaggtgg
     4801 ctccgagttc ctcgcagggc gtaaggtcgc ttgcgggaat gtgtttgggc ttcaggatat
     4861 cttccgtgct ggtggcatga gtgcagacga tgatcggctc ggcgatcgcc ttgaggcgcc
     4921 aaccggtggc gatgcgctcg atctgttgcg ccgcactccg gccgtcgctg cctgcgcaaa
     4981 tgagaacggc atacggccgg ccattcagcc gctcgagcag cggatagtac gtgcgatcga
     5041 agaaatcctt catctggccc gacatggccg cgagattctc agggcttgcg aagatgtagc
     5101 cgctcgctgc gagcacatcg tctgcgtgca cctcggcggc cgcgcgtaag gagacgtcga
     5161 gcgccgtctc ggcttgggcg ccgcgcgccg cggcgcgcgc catctgcagg gttcctccgg
     5221 tcagggagtg ataaacgatc aacagggtct tcatgaggac gattctcgca aacctcccgt
     5281 gggccgtatc ctacagaggg cgacgatgcc aaccgactgt aaagccgcgg gggttgttcg
     5341 acactcccgc tcagccgcaa ccgctttcgt caacaaaagg tgtcatcatg cttcactatg
     5401 ccgtagtttt ctttatcatc gcactgatcg ccgctgtctt tggcttcggc ggaattgcgg
     5461 tcggtgccgc cggtattgct aagatactgt tcatcgtatt tctcatcggc gccgtggtca
     5521 gtttcttgat gagcctcggc cgccgcgtct gacgtcttgc atcatccata ggagcacaca
     5581 tggaatccgt aatcgaatcg ccgacggcgc gtaagatcag cgactatgcc aatgggcagg
     5641 tcaataattt tctcgaaagc gtcgaggatt tgaccaaggc attgaaggac atcgaaacgc
     5701 ctgacattgc gcgggttcgt gccaaggtga agattgcgtt ggtggcagcc aagagtgccg
     5761 tgaccgacac ggctcaacaa ctgggtagtc aggcgaaaca ggtcggcaaa cggaccgacg
     5821 atttcgtgcg cgacaatccc tggcaggtca tcggcattgc ggcggtggtg ggtctggcgg
     5881 tcggcatcct ggcgtcgcgc cgttcctgag cggacttgag tggcggcgcg gcctatgcgc
     5941 gccgccactg cgtgaccccg ccgggcttgt cctcgagcaa gattcccgcc gcggtcagct
     6001 gctcgcgaat gcggtcggac tccgcgaaat ttttggcggc ccgcgcgctg cgccgcgccg
     6061 cgagcaacat ctcgatttcc tcatcggcta tggatttctc gcccacgctg cgcttcaagt
     6121 acatcgcggg atcttgctgc aacacgccga ggaccgcgcc catcgcgcgc agcgccgcgg
     6181 cggccgacgc cgcggccgca tcgttccccg cggccttggc ttgattgagc tcgcgggcca
     6241 ctccctgcat caccgcaagc gcttcgggcg tgttgaagtc atcgtccatc gcgcggcgga
     6301 attcgccgag tgcggcagga tcgaccgtcg ccgcgcccgc atccttcagg gcgcgataca
     6361 gacctaacaa ggtctcatcc gcctgcgcga gctgcacgct cgagtaattg atgggtccgc
     6421 ggtagtggct gctgagcaag aagaagcgca gcacttcagg atcgcgcaac gtcttcagaa
     6481 cctcacgcac agtaaagaaa ttgccgagcg atttcgacat cttctcatcg ttcacgcgca
     6541 cgaacccgtt gtgcatccaa acgtttacaa acggggtgtc gcaagcagca caggactgcg
     6601 cgatctcgtt ctcatggtgc ggaaacttca gatcctcgcc gccgccgtgc aggtcgaaat
     6661 agccgccaag caagctggtc gacatcgccg aacattcgat gtgccagccg ggccggcctt
     6721 ggccccacgg cgaatcccag tgaggctcgc ccggtttggc gtgcttccac agcacgaaat
     6781 cgagcgggtc gagcttggcg tcatcgacct gcacgcgcga gccggcgcgc aagtcgtcga
     6841 tctttttccc cgacaggcgg ccgtactgcg gaaattttcg caccgagtac atgacatcgc
     6901 cgttggcggc aacataggcg tatcccttgt cgatcaacgt ctgcgtcatg gcgatgatcg
     6961 ggcctatgta gtcggtggca cgcggctcga gatcgggcga ctgcagtccc aacgtggcgc
     7021 aatcctcgtg catggctacg gtgaaacgcc gcgccagatc cgcccagttc tcgccgttgg
     7081 cggcggcgcg ctcgatgatc ttgtcgtcga tgtcggtgat gttgcgtacg taggtcacgt
     7141 tgtagccgag cgatcgcagg taacgttgaa tcagatcgaa taccgtcagc atgcgtgcat
     7201 gcccgacatg gatgtagtcg tagttggtca tgccgcacac gtacatgcgc acttcattgc
     7261 cacgcagagg tttgaacagc gctttctcgc cgctgagtga gttatgaatt ttgagcatga
     7321 gattcgaggc gatggcgtgc cttctccggt gacagcagtt tgagcaacgg tgcgagttcg
     7381 ggaccgtgag cctgaccggt cagggcgaca cgcaaaggca tgtaaaggtc ggcgcccttg
     7441 cgcccggtgc gttctttcag aatcctggtc aattgcttga gatccgaggg tgcttgatcg
     7501 aacgcgtcga cagcggcggc gaaaaacttg ggtcccgccg ccgcgacgac agcctgctcg
     7561 tcggttccta acggcggcag gtctccctgg actaccgcgc gccatgcgac cgcgtcggcg
     7621 ggcaacacca cgttgtgccg caccaactcg ataaaggcgc tcgaatcgcc gcacccgagc
     7681 cacgcggcga gttcggcgct cgacatgcgt tccaccgtct ccttttgcca atgcgccaat
     7741 tgcgtctcgt cgaagcgcgc gggcgcgcgg cccaggtgtt cgaagcgaaa gtgctgcggc
     7801 atggcggacg gcggcaacca gccgtcaacg tcgctcgcat gccccagtcg gaacagatga
     7861 ttgagcacgg cggcgggcag gaatccgcgt tcgcgaaatt ccagggcact ggtactgccg
     7921 tgccgcttcg acagcggcgc gccgtccgcc ccgaccagca agccgacatg accgtagccg
     7981 ggccgcctca tctgcaacgc gtcgagcacc atcagttggc gcggcgtatt cgtgagatga
     8041 tcatcgccgc gcaatacgtg cgtgactccc atgagcgagt cgtcgacggc attgcaaaag
     8101 aagaaccccg aggtaccgtc gtcgcggcgg atgatgaagt cgcctatatc gttcgacaga
     8161 aatcgctgcg ggccgtgtac cacgtccaga aactcgacga tttggtcggc tggcaccgca
     8221 aatcgaagag tcggcttgag ac
//
LOCUS       DASWZT010000121         7425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_63806, whole genome shotgun sequence.
ACCESSION   DASWZT010000121 DASWZT010000000
VERSION     DASWZT010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7425
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63806"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VGE92_03290"
     CDS             <1..988
                     /locus_tag="VGE92_03290"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEY1312870.1"
                     /translation="LGTWAPPPDDAADDQIGDELLSLIFVACHPVLSTEARTALTLRL
                     LGGLSTAEIARAFLVPEATIAQRIVRAKRTLAEARVPFEVPCGAPRALRLNSVLEVIY
                     LIFNEGYSATAGDDWMRPALCEEALRLGRILAELLPSEPEVHGLVALMELQSSRTGSR
                     VDAAGKPILLLAQNRGRWNQLLIQRGLAALARAEAQAGALGPYALQAAIAACHARARA
                     AEQTDWGRIVALYDALAELTPSPVIELNRAVAVSMAFGPAAALELVDALTLEPRLQGY
                     HLLPSVRGDLLEKLGRFEEARAEFARAAAVVQNERERTLLLGRASACGREVQ"
     gene            1032..1403
                     /locus_tag="VGE92_03295"
     CDS             1032..1403
                     /locus_tag="VGE92_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312871.1"
                     /translation="MVKRTLIWLLPALLAPLYASSARADNFGRVYYDAKNDQLVVTMI
                     YRGTNPNHNFSLKWGACHAGQSGGAQEVSVEVLDDQFQDRAQQNFKTTKNFSLQGLTC
                     RPAKVTLRTAPRFLYTLTIPG"
     gene            1423..2082
                     /locus_tag="VGE92_03300"
     CDS             1423..2082
                     /locus_tag="VGE92_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY1312872.1"
                     /translation="MRVLLVEDDGMIAQGLQTALRQAGFAVDWMRDGKSAGAALQSAS
                     FDLVLLDLGLPQRDGIEVLRDLRKRGNVTPVIILTARDEIQHRIAGLDAGADDYIVKP
                     FDVDEVTARMRSVLRRAAGRGDPSIQYGDLRLDPVTHTVERDGVAVALSGHEFAVLEA
                     LLQRPGAVLSRSQIEDRLYGWDEQIGSNAVEVYIHGLRRKLGSDAIRTLRGVGYYVPK
                     L"
     gene            2079..3383
                     /locus_tag="VGE92_03305"
     CDS             2079..3383
                     /locus_tag="VGE92_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1312873.1"
                     /translation="MKSIRARLLIALIMLVALISLLAAGVTYRRVLSETSTLFDYQLR
                     QMALSLRNQISLAPRIEVPPDQGDADFVVQIWDVFGARTYLSRPGLPMINQTVLGYAD
                     LSVRGQAWRAYGLQTADGVIQIAQPARVREALARAAAERVVIPLILLLPIMIVAVAWI
                     VRRGLSPLQYVAAEVQRRDVRSLTPLAADNLPREIAPLVGELNRLLARLQDAFAVQRA
                     FISDAAHELRSPLTALRLQLQLLDRAPDEAARLEARNRLGAAVERAIHLVEQLLTLAR
                     SDPQEAAGDFGSVNLAAVAADSITDTHDLALARSIDLGLEAAPNVPVRGDREALRTLI
                     RNLVDNAVRYTPRHGSVQIRCRTTAAGAQLEISDTGPGISPADRHRVFDRFYRKTDSE
                     ETGTGLGLAIVKAIAERHGARIRLGTAPAGGLQVELSFPAAS"
     gene            3450..4925
                     /locus_tag="VGE92_03310"
     CDS             3450..4925
                     /locus_tag="VGE92_03310"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEY1312874.1"
                     /translation="MQLRNRTLAWSAAGLFAVGTAAGLSAPTLLHAGASPAAEPVAAN
                     STASSAQPIPLITAPNYRAIVARNQAAVVGITTAGPMNTAVPQEFNFGGPDGDDDNPL
                     SKFFQGIPRPRQRGMMHAQGSGFLISADGLVLTNAHVVDGAKQVTVKLADHREFKAKV
                     LGADRSSDIAVLKIDAHDLPSVHLGDSDQLSVGDYVLAIGEPFGLEETATAGIVSAKG
                     RSLPGDGYVPFIQTDAAVNPGNSGGPLFDANGAVVGINAQIYSNSGGYQGVSFAIPIN
                     LAVQVKDQIVKTGKVAHSRLGVEVQTLDQSLADSFKLKTPNGALVAKVTPDSAAAAAG
                     IKVGDVILKFNGSPIIDAGQLSQRVGAVAPGEKASLEIWRDGKTLSLSATVGNAAQVA
                     DASESPSGANAPARLGLALRPLNPAERQQAGVAGGLIVEDSQGHAAEAGIQPGDVVLS
                     VDGTPVESVAQLRKMVQQHDKQIALLIQRGDARLFVPVNLG"
     gene            5246..6115
                     /locus_tag="VGE92_03315"
     CDS             5246..6115
                     /locus_tag="VGE92_03315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEY1312875.1"
                     /translation="MATPARSIASLTISFGLVAIPVKLYSATVSAERISFNLLRQKDG
                     SRVKQQYVAVNDGKIVERSEMVKGYEFAKDQYVMFSAEELKALEDATTHAIDIGQFVP
                     LASVDPVYFDGTYYLAPDKGGAKPYTLLATALRKADQCAVGRWISRGKEHIVVIRALQ
                     DGLAMHQLHFQAEVRNLKDLGVEAAPVSEPELKLARQLIDHLAAKRFDPNEYFDEHKA
                     RVEAAIQRKVDGKEVSLAEGPVSNTGGNVIDLMEALRASIDARGSKAADLKERKAPKR
                     AAAQASSRKTARR"
     gene            6072..6926
                     /locus_tag="VGE92_03320"
     CDS             6072..6926
                     /locus_tag="VGE92_03320"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1312876.1"
                     /translation="MQQPRHHRAKPLDDEMHAYNSKDLERLFGLPASAVRSLARAGHI
                     SPIKRAGRLHYSFQDLLMMRTASALRAANISAHRINRTLETFRETLPTGAASNQRSLT
                     AMGNQIAIREGKMLWESESGQYALDLGEDKGELHVISRPLPAAPVAKSADELYAQAFA
                     LEDTDPDAACAIYEAALEVDPEHVEARINFGRLLHMAGRLSEAERVYRGASKPESLII
                     FNLAVVLEDLKREPDAIGAYRESLALDPGLADAHFNLARLYERAKNPKASLRHLLAYR
                     RMMDRQGT"
     gene            6926..>7425
                     /locus_tag="VGE92_03325"
     CDS             6926..>7425
                     /locus_tag="VGE92_03325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEY1312877.1"
                     /translation="MAKPALSVYRAKRDFSKTAEPSGKARVAPSPRLRFVIQKHAARQ
                     LHYDLRLELNGVFKSWAVTRGPSVDPADKRLAVEVEDHPLDYGDFEGTIPKGEYGGGT
                     VQLWDRGYWSPEGGKSAQSAFDSGDLKFTLQGSRLHGSWVLVRMRADRYGGKRTNWLL
                     IKHRDAG"
BASE COUNT         1407 a         2275 c         2339 g         1404 t
ORIGIN      
        1 gctgggtact tgggcgcccc cgcccgacga cgctgccgac gatcagatcg gcgatgagct
       61 cctcagtctg atattcgttg cctgccaccc ggtgctatcg accgaggcgc gcacggcgct
      121 gacgttgcgc ttgctcggag gcttgagcac cgccgagatc gcacgcgcgt tcctggtgcc
      181 tgaggccacg atcgcacagc gaatcgtccg cgcaaaacgc acgttggcag aagcccgagt
      241 tccctttgag gtgccctgcg gcgcgccgcg cgccctgcgc ttgaattcgg tactcgaggt
      301 gatttacctt attttcaacg agggctactc ggcgaccgcc ggcgatgact ggatgcgccc
      361 ggcgttgtgc gaagaggcgc tgcgcttggg tcggattctt gccgaacttc tgccatcaga
      421 gcccgaagta cacggtctgg tcgcgctcat ggaactgcaa tcatcgcgga ccggctcgcg
      481 tgtcgatgcc gcgggcaagc cgattctgtt gctcgcgcag aatcgcggac ggtggaacca
      541 gctgttgatc cagcgcggac tcgcagcgct cgctcgtgcc gaggcgcagg cgggtgccct
      601 cgggccgtat gcgctgcaag ccgccattgc tgcctgccat gcaagggcgc gagccgcaga
      661 gcaaaccgat tgggggcgaa tcgtcgctct ctacgacgcc ctcgcagaac tcacgccctc
      721 tccggtgatc gaattgaatc gtgccgttgc cgtctcaatg gcattcggac cggcggcggc
      781 gctcgagctc gtggatgcgc tgacccttga accgcggctc cagggctatc acctgttgcc
      841 gagtgtacgc ggcgatctac tcgagaagct cggacgcttt gaagaggccc gcgcggaatt
      901 cgcgcgcgcg gcagcggtcg tacagaacga gcgcgagcgc acgctgctgc tcggccgcgc
      961 atccgcgtgc ggccgcgagg tgcagtagcg ccgattgcgg tattcttttg cgaatcattg
     1021 gagggtcggt catggtcaaa cgaaccttga tttggttgct gcccgcgctg ctcgctccac
     1081 tttatgcgtc gagcgctcga gccgacaact ttggcagggt ctattacgat gccaagaacg
     1141 atcagttggt cgtcacgatg atctatcgcg gcaccaaccc gaatcataat ttctcgctca
     1201 aatggggcgc gtgccatgcg gggcaatcgg gcggcgcgca ggaagtgagc gtcgaagtgt
     1261 tggacgatca atttcaagac agggcgcaac agaatttcaa gaccacgaag aatttttcgc
     1321 tccaggggct cacctgccgt cccgccaagg tgactttgcg aaccgcgccg cgattcttat
     1381 atacgctgac cattccgggg tagccggcac cgggcgctcg ccatgcgtgt gttgctcgtc
     1441 gaagacgatg gcatgattgc gcagggcttg caaacggcgc tacgccaggc cggattcgcg
     1501 gtggattgga tgcgcgacgg caagagcgcc ggcgcagcat tgcagtctgc gagtttcgat
     1561 cttgttctgc tcgatctcgg gttgccgcag cgggacggaa tcgaggtctt gcgagacctg
     1621 cgcaagcgcg gcaatgtcac tccggtgatc atcctgacgg cgcgcgatga aatccaacat
     1681 cgcatcgccg ggctcgatgc cggggccgac gattacatcg tcaagccttt cgatgtggac
     1741 gaagtaaccg cgcgaatgcg ttccgttctg cgccgtgccg ccggcagagg cgacccgagc
     1801 attcagtatg gcgacctgcg tcttgatccg gtcacccata ccgtcgaacg cgacggtgtt
     1861 gcggtggcct tatcgggaca cgaatttgca gtgctcgaag cgcttctgca gcggccgggc
     1921 gccgtgttgt cgcgcagcca gatcgaagat cgattgtacg gttgggatga acaaatcggc
     1981 agcaacgccg tcgaagtcta tatccacgga ttgcgccgca agcttggcag cgatgccatc
     2041 cgaacactgc gcggtgtcgg ctactacgtc ccgaaattat gaaatccata cgtgcccgat
     2101 tgttgatagc gctgatcatg ctcgtcgcgc tgatttcact gctggccgcc ggcgtcacgt
     2161 atcgacgcgt gttgagtgag acctcgaccc tgttcgacta tcagctgcgg cagatggcgt
     2221 tatcgctgcg caaccagata tcgctggcgc cgcgcatcga agtgcctccg gatcaaggcg
     2281 atgccgattt cgtggttcag atctgggatg tcttcggtgc gcgaacctat ctgtcgcgcc
     2341 ccggcctacc gatgatcaac caaaccgtcc tgggttacgc ggatctgtcc gtgcgcggcc
     2401 aggcgtggcg cgcctatggc ctgcaaacgg cagacggggt gatccaaatc gcacagccgg
     2461 cgcgggtgcg cgaagcgttg gcgcgcgccg ccgcggaacg cgtcgtcata ccgctgatct
     2521 tgctgctgcc catcatgatt gttgcggtcg cctggatcgt ccgtcgcggt ctatcgccgc
     2581 tgcagtatgt cgccgctgaa gtgcagcgcc gagatgtgcg cagcctaacc cccttggccg
     2641 ccgataatct gccgcgcgaa atcgcgccct tggtggggga actcaaccgg ttgttggcgc
     2701 ggctgcaaga cgcgtttgcc gtgcagcggg cttttatttc cgatgccgcg catgagctgc
     2761 gatctccgct cacggcgctg cggctgcagc tgcagctgct tgaccgtgcg cccgatgagg
     2821 ccgcccgcct cgaagcgcgc aaccgactcg gcgccgcggt cgagcgcgcg attcacttgg
     2881 tggagcagct cttgaccttg gcgcgcagcg atccgcaaga ggcggcgggc gacttcggct
     2941 cggtcaatct ggcggcggtc gctgcggata gcatcaccga cactcacgat ctggctctgg
     3001 cgcgtagcat tgatttgggt ctagaggccg cgccgaatgt cccggtgcgc ggtgatcggg
     3061 aggcgctgcg aaccctgatt cgcaacttgg tcgacaatgc ggtgcgttac acgccgcggc
     3121 acggatcggt ccaaattcgc tgccggacga cggccgcggg cgcgcagctc gagataagcg
     3181 acacagggcc cggtatcagc cctgcggacc ggcatcgggt gttcgatcgc ttctaccgaa
     3241 agaccgactc ggaagagacg ggtaccggac tcgggctcgc cattgtgaag gccatcgcgg
     3301 agcgtcacgg cgcacgcatc aggctcggca ccgcgccggc cggcggcttg caggttgaat
     3361 tgagctttcc ggcggcatct taagactgcc ttaagcatgg cgtgggaacc tgcgctcaga
     3421 gcacttgctc catatcgcag gagacccaca tgcaacttcg aaacaggacg ctggcatggt
     3481 ccgcggccgg cttgtttgcc gtcggtaccg ccgcaggatt gtcagccccc acactgttac
     3541 acgccggcgc ttcgccggcg gctgagccgg tggccgctaa ctcaaccgcg tcctcggcac
     3601 agccgattcc gctcatcacc gcgccaaatt atcgggcgat cgtcgcgcgc aaccaggccg
     3661 ccgtcgtagg catcaccacg gcgggtccga tgaacaccgc cgtcccgcaa gagttcaact
     3721 tcggcgggcc cgatggcgat gacgacaatc cgctatcgaa gttctttcag ggaataccca
     3781 ggccgcgcca gcgaggcatg atgcatgcgc aaggctcggg attcctcatc tccgccgacg
     3841 gccttgtgct caccaacgca catgtcgtcg acggtgccaa acaagtcact gtcaaattgg
     3901 ccgatcaccg cgaattcaaa gcgaaagtgt tgggcgccga ccgcagcagc gatatcgcgg
     3961 tgttgaaaat cgatgcccat gacctgccga gcgtgcatct cggggactcg gaccaactga
     4021 gcgtgggcga ctacgtgctc gcgatcggcg aacccttcgg tctcgaggaa accgcgaccg
     4081 ccggcatcgt gagcgccaaa ggccgctcgc tgccgggcga cggctacgtg cccttcattc
     4141 agaccgatgc ggcggtcaat cccggaaatt ccggtgggcc gctgttcgac gccaacggcg
     4201 ccgtcgtcgg catcaatgcg cagatctact cgaactccgg aggctaccaa ggcgtctcgt
     4261 tcgccatacc catcaacctc gccgttcaag tcaaagacca aatcgtcaaa acgggtaagg
     4321 tggcgcactc gcgtctcgga gtcgaagttc agactctcga tcagtcgttg gccgattcct
     4381 tcaaattgaa gacgccgaac ggcgccctcg tggcgaaggt cacgccggac agcgcggcgg
     4441 cggcggcggg catcaaggtc ggcgacgtca tcttgaagtt caatggcagc ccgattatcg
     4501 acgccgggca gttgtcgcag cgggtcggag cggtagcgcc gggcgagaag gcctcgcttg
     4561 agatctggcg cgatggcaag acgctgagtc taagcgcgac ggtcggcaat gcggcgcaag
     4621 tggcggatgc ctccgaaagc ccgtccggcg ccaatgcacc ggctcggttg ggcttggcgt
     4681 tgcgaccctt gaaccccgcc gagcgccagc aagcaggtgt tgcgggagga ctgatcgtcg
     4741 aggactcgca aggccacgcg gcagaagcgg gcattcaacc cggcgacgtc gtgctgtcgg
     4801 tcgacggcac accggtcgag agcgtcgcgc aactgcgcaa gatggttcag cagcacgaca
     4861 agcagatcgc gctgctcata cagcgcggcg atgcgcgttt gttcgtcccg gtgaacctgg
     4921 gctagagaat agaatcgaca ggcttgccaa caggctcacc cagggcgcca cagatgcgcc
     4981 ttgggtgagc cctgtcatgt cagccgcctg ccgcggcgtc gagatagctc ttctaagcat
     5041 ctgttctaaa tagaattaat cgcttttaac aggtctaaca acgagccggc tttagccgcc
     5101 atcgccgcgt cggcgtgcgc cctatcgggc tcatccaggc atcctacaag tgagcgctaa
     5161 gtctcgttaa aataatgtgt tgcgctcttt tcattacatg aattagagtt tgaccataac
     5221 tgaggctttc tgggatggtt ggcatatggc tactccggct cgatcgattg cgtcgctcac
     5281 aatttccttc ggcttagtgg ccattccggt caagctgtat tccgcgacgg tttcggccga
     5341 acgcatctcc ttcaacctcc tgcgacaaaa ggacgggtct cgagtcaagc agcaatacgt
     5401 ggccgtgaat gacggcaaga tcgtcgagcg atcagagatg gtcaagggtt acgagttcgc
     5461 caaggaccag tacgtgatgt tcagtgccga agagctaaaa gcgttggagg atgcgaccac
     5521 gcacgccatc gacatcggtc aattcgtgcc cttggcctcc gtcgacccgg tttatttcga
     5581 tggcacttac tacctggcgc cggacaaggg cggcgcgaag ccctacaccc tgctcgccac
     5641 cgccttgcga aaggcggatc aatgcgcggt cggccgctgg atctcgcgtg gcaaggaaca
     5701 catcgtggtc atccgcgcgc tgcaggatgg cttggccatg catcaactgc atttccaagc
     5761 ggaagttcgc aatttgaagg acttgggagt cgaagcggcg ccggtgtcgg aacccgaatt
     5821 aaagcttgct cgtcagttga tagaccattt ggcggcgaag cgctttgatc cgaacgaata
     5881 tttcgatgag cacaaggcgc gcgtcgaagc agcgatccaa agaaaggtcg acggcaagga
     5941 ggtttcgctc gcggaaggac cggtttcgaa tacaggtggc aacgtcattg acttgatgga
     6001 agcactgcgc gccagcatcg atgctcgagg gtcgaaggcc gccgatctta aggaacgaaa
     6061 ggcgcccaaa cgtgcagcag cccaggcatc atcgcgcaaa accgctcgac gatgagatgc
     6121 atgcctataa ctcgaaggac ctagagcgtc tttttggatt gccggcctcc gcggtgcgct
     6181 ctctggcgcg cgccggccac atcagcccca tcaaacgcgc gggacggctg cactactctt
     6241 tccaggatct tctgatgatg cgcacggcga gcgcgctgcg cgctgcgaac atttcggcgc
     6301 atcggatcaa ccgcacgctg gagacgtttc gcgaaaccct gccgaccggc gcggcatcca
     6361 atcagcgctc gctcactgcg atgggcaatc agattgcgat tcgcgagggc aaaatgctct
     6421 gggagtccga gtccggccaa tatgcactcg acctgggtga ggacaagggc gaattgcacg
     6481 tcatcagccg gccgctcccc gcggcgcccg tagcgaaatc ggcggacgaa ctctacgcgc
     6541 aagcctttgc gctcgaggac acggacccgg acgctgcctg cgccatctat gaagccgcgc
     6601 tcgaggtcga tcccgagcac gtcgaggcgc gcatcaattt cggccggctg ctgcatatgg
     6661 ccggccggct cagcgaagcg gaacgcgtgt atcgcggcgc ctcgaaaccg gagtccttga
     6721 tcatcttcaa tctagcggtg gtactcgaag atttgaaacg cgagccggac gccatcggtg
     6781 cctatcgcga atctctcgcg ctcgaccctg ggctggcgga cgcgcacttc aatctggcgc
     6841 ggctgtacga gcgggcgaag aaccccaaag cgtcgttgcg ccatctgctc gcgtaccgcc
     6901 gcatgatgga tcgacaagga acctgatggc gaagccggcg ctctcggttt atcgggccaa
     6961 acgcgacttc tcaaagacgg ccgagccatc gggcaaggca cgcgtggccc cctcgcctcg
     7021 gctgcgcttt gtcatccaga agcacgcggc gcggcaactg cattacgatc ttcggctgga
     7081 attgaacggc gtgttcaaat cctgggctgt cacccgcggc ccctccgtcg accctgcgga
     7141 caagcgcttg gcggtggaag tcgaagatca tcctttggac tacggagatt tcgagggcac
     7201 gattcccaag ggcgaatatg gcggcggcac ggtgcaactg tgggaccggg gctattggtc
     7261 gccggagggg ggcaaatccg ctcaaagcgc cttcgacagc ggtgatctga agttcactct
     7321 gcagggatca cgcctgcacg gcagttgggt cttggttcgc atgcgggcgg atcgctacgg
     7381 cggcaagcga accaattggc tcttgatcaa acatcgagac gcggg
//
LOCUS       DASWZT010000122         1981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_63813, whole genome shotgun sequence.
ACCESSION   DASWZT010000122 DASWZT010000000
VERSION     DASWZT010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63813"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VGE92_03330"
     CDS             <1..465
                     /locus_tag="VGE92_03330"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEY1312878.1"
                     /translation="ADIRPYRVLIDNNLPGIMAAHVVFPAVDALPASLSPRWINGVLR
                     RELNFHGCVFADDLSMAGAAAFGDVIGRVELAFAAGCDVLPICNARHSVEFVLGRLAA
                     DVGSAASQARLVRMRARGEAPVNLQTNRQWQETAARIAGLSDIPPLVLTEGQ"
     gene            465..1397
                     /locus_tag="VGE92_03335"
     CDS             465..1397
                     /locus_tag="VGE92_03335"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY1312879.1"
                     /translation="MTGFDADLYRRLVETSPEGVVVVDAQNPEQPVIYANPGFEALTG
                     YAAAELVGRNLRFLQADDREQDGRNRLREALGRGEPCRVLLRNYRKDGTVFWNEMTAL
                     PLRDAEGRITHFVGHHRDAGERLRIDPKLTRDSLSGAHQPTAVAIRDDRLTGLFSLPY
                     LEELLKRDWAVAQREKRSIAVFAIDIDALDLYNATFGRGAGDSMIRRVAHVVSGCLRR
                     SSDVTARIDGGSLMAFAPGLNDEQGLRIGQLMAERVRDLRIHHPRSAILRYVSISVGV
                     CAAIPQPSDSPSILVEKSQERLHFAKNAGRNQAA"
     gene            complement(1460..1801)
                     /locus_tag="VGE92_03340"
     CDS             complement(1460..1801)
                     /locus_tag="VGE92_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312880.1"
                     /translation="MLNSARKHLSFAAVLVSLAALAGTASAETTWQKNHPRRTQVNHR
                     LNNQNKRIHQDVKDGTLSKSQAAGLHKEDHQVRQEERDMAGQNGGHISKGEQKVLNGQ
                     ENQISKQIPPK"
BASE COUNT          332 a          589 c          640 g          420 t
ORIGIN      
        1 gcggatatcc gcccctatcg cgtactgatc gacaacaacc tgccggggat catggcggcc
       61 cacgtggtgt tcccggccgt cgacgcacta cccgccagct tgtcgccgcg atggataaat
      121 ggggtgctgc gccgcgagct caattttcat ggctgtgtgt tcgcggacga cctttcaatg
      181 gccggagcgg cggcttttgg cgatgttatt ggccgcgtcg agctggcttt tgctgcgggt
      241 tgcgacgtcc tgcccatctg caatgctcga cattccgtag aattcgtgct tggccgtttg
      301 gcggcggacg tcggctctgc ggcatcgcag gcgcggctcg tgcgcatgcg tgcccgcggc
      361 gaagcgcctg tgaatttgca gacgaaccgg cagtggcagg agacggcggc gcggatcgcc
      421 ggtctgtcgg acattccgcc gttggtgttg accgagggac aatgatgacg ggctttgacg
      481 cggatctgta tcggcgctta gtcgaaacct cgcccgaggg cgtcgtcgtc gtggatgcgc
      541 aaaatccgga gcagccggtc atctatgcca atcccggatt cgaggccttg accggctatg
      601 cggcggcgga attggtcggc cgcaatctgc gcttcttgca ggccgatgat cgggagcagg
      661 atggccgcaa ccgattgcgc gaagcgctcg gccgcggcga gccgtgccgt gtgctgctgc
      721 gaaactaccg taaggacggc acggtatttt ggaatgaaat gacagctctg ccgctgcgcg
      781 atgcggaggg gcgcataacg cacttcgtcg ggcaccatcg ggatgcgggc gagcggctgc
      841 gaatcgaccc caaactcacc cgcgattcgc tgagcggcgc gcatcagccg acggcggtgg
      901 ccatccgcga cgatcgcctg accggactct tcagcctgcc ctatctggaa gagctgctga
      961 aacgcgattg ggcggtcgca cagcgggaaa aacgcagtat tgcggtgttc gcaatcgaca
     1021 tcgacgccct cgacctgtac aacgcaacgt tcggccgcgg cgcgggcgat tcgatgattc
     1081 gccgtgtggc gcatgtggta tccggatgtt tgcgccgctc cagcgatgtg acggcacgga
     1141 tcgacggcgg cagcctcatg gcgtttgcgc cggggctcaa cgatgagcag ggattgcgga
     1201 tcggtcagct catggccgag cgcgtgcggg atctaaggat tcaccatccg cggtcggcga
     1261 ttctgcgcta tgtcagcatc agcgtcggtg tctgcgccgc gattccgcag ccctccgaca
     1321 gtccgtccat tttggtggaa aaatcgcagg agcgattgca cttcgccaag aacgcgggcc
     1381 gcaaccaagc cgcttgaggg cgcatcgccg gggaagcgcg ctgcgcgctt ccccgtatgg
     1441 cccagcgaac ttgaggcaat tacttcggcg ggatctgctt gctgatctga ttttcctggc
     1501 cgttcagaac cttctgttcg cccttgctga tgtgtccacc gttctgaccg gccatgtcgc
     1561 gctcttcctg gcggacctga tggtcctctt tatgcaagcc cgcggcctgc gacttgctga
     1621 gcgtgccgtc cttgacgtcc tggtgaatgc gcttgttttg gttgttcaac cgatggttta
     1681 cctgcgtacg gcgcgggtga tttttctgcc acgtggtttc cgccgatgca gtgccggcga
     1741 gcgcggccaa cgagaccagt acggccgcga aactcagatg tttgcgtgct gaattcaaca
     1801 taatcattcc tccccgcgat gtgcgttcga tccgtataag aagccaagta agcggctctt
     1861 ggcgtgaagt aacgcggcag tctgcgattc ggctacgcaa attacgcact gttaattcgt
     1921 atatcggccg cgctgcatcg tgatccaagt ctcatcctcg gcggattcgc cgtggcgcac
     1981 g
//
LOCUS       DASWZT010000123         8472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_87897, whole genome shotgun sequence.
ACCESSION   DASWZT010000123 DASWZT010000000
VERSION     DASWZT010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8472
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87897"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VGE92_03345"
     CDS             <1..278
                     /locus_tag="VGE92_03345"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312881.1"
                     /translation="SHQLQGERAGLINRQFDTAFLDGLTQNPLALTRISHTEYMREAG
                     SEGVEMVMWHVMRGALDDDAVEVYRHYHVPASNTAAGLLILENRSSP"
     gene            275..1219
                     /locus_tag="VGE92_03350"
     CDS             275..1219
                     /locus_tag="VGE92_03350"
                     /inference="COORDINATES: protein motif:HMM:NF040065.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY1312882.1"
                     /translation="MKVCLAGHGAFGIKHLEAMAKIPGIEVISLAGSNLDSAREVAQQ
                     WKIPHWTATLAESLAQPGLDAVILTTPTQMHAAQGVESMRAGKHVMIEIPMADSVADS
                     ELLVRVQKETGVVAMAGHTRRFNPSHQWVHKRILAGELKVLQMDVQTYFFRRTNTNAL
                     GKPRSWTDHLLWHHACHTVDLFGYQTGERISQEFALQGPKHPVLGIAMDMSIGLKVPS
                     GALCTLSLSFNNDGPLGTFFRYICDNGTYIARYDELLDGKNNPIDVSKVDVSMNGIEL
                     QDREFFAAIRDQREPNSSVAQVLPAMLTLGRLEQQLGK"
     gene            complement(1240..2415)
                     /gene="pobA"
                     /locus_tag="VGE92_03355"
     CDS             complement(1240..2415)
                     /gene="pobA"
                     /locus_tag="VGE92_03355"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019371336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018659 - 4-hydroxybenzoate
                     3-monooxygenase activity [Evidence IEA];
                     GO_process: GO:0018874 - benzoate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HEY1312883.1"
                     /translation="MARTQVAIIGAGPSGLLLSQLLYLHGVDNVVLERQSREYVAGRI
                     RAGVLEQGTVELLLQAQVGERMLRERLVHDGFALGFAGQTFRVDLKRFAGNAVSVYGQ
                     TEVTKDLMDARAAAGATMIYEAGEVSIEGFDRARPTVRYVQDGRTHELSCDFVAGCDG
                     YHGVCRRSVPQTALQSFERIYPFGWLGILADVPPAAHELVYSNHERGFALCSMRSLNR
                     SRYYVQCGLDEKVEAWSDARFWEELRSRLPVQVADAIVTGPSIEKSIAPLRSFVVEPL
                     KFGQLFLVGDAAHIVPPTGAKGLNLAAADVRVLYQALVEYYRTGSAALIDRYSARCLR
                     RIWKAVRFSWWFTSVTHKFSDDAFAHRLQLAELDYLTGSTAALASLAENYVGLPFED"
     gene            complement(2475..3368)
                     /locus_tag="VGE92_03360"
     CDS             complement(2475..3368)
                     /locus_tag="VGE92_03360"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY1312884.1"
                     /translation="MRARRRAIPSFSLYGETPRAAQTDGIHIEDIQSRSRKYLWRIGT
                     HRHSLLSQCVVVTSGPVIVTLDDSRAALAGPAVMIIPAGTVHSFRFRADTQGHVLTID
                     LAQVLSAATPLQRSPVETLFAIARAIDLQDEPLLAQRLEQLLELLEQEFRQPDSWAGP
                     IGGWLACCLLWILAQGCAAGDATELPSGLDAGRLRRFRQLIESQYLKHWPVARYARSL
                     ALSETSLNRLCRRLAGATAFDLIQQRLALEARRRLVYAGSSVSGIAAELGFKDPAYFC
                     RFFRRHNGSSPLNFRRRHHGG"
     gene            complement(3373..3882)
                     /locus_tag="VGE92_03365"
     CDS             complement(3373..3882)
                     /locus_tag="VGE92_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312885.1"
                     /translation="MLAADDPRQTEAPGGEKLQIGRWRLTVRAHYRITARILGLERYH
                     FDALADLIPEDLALGWGPMSDSRILRGIEITQSNRFYSWRARRNAPIPKDSIIVHSAN
                     THVIPRDVSIARQLSRLRPGEIVTLSGDLVDGVRDDGTWINTSLVRTDTGAGACEVLL
                     VSDVALVSE"
     gene            complement(3982..4317)
                     /locus_tag="VGE92_03370"
     CDS             complement(3982..4317)
                     /locus_tag="VGE92_03370"
                     /inference="COORDINATES: protein motif:HMM:NF013517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HEY1312886.1"
                     /translation="MQSRISRRAVVKGGLIAGAMIPAMGLMTQSSQAAGLTALDPKDP
                     TAQALGFVTDATKVDAAANATYKAGQKCSTCAQYQGKASDATAGCNIFAGKSVPAAGW
                     CKVWAAKPA"
     gene            complement(4386..5327)
                     /locus_tag="VGE92_03375"
     CDS             complement(4386..5327)
                     /locus_tag="VGE92_03375"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY1312887.1"
                     /translation="MSQIRPVVGLPTLTWLRAFEAAARTSSFAAAAAELNLTSGAISY
                     QIRGLEAHLGFALFERLPRGVKLTPMGVAYLPPVRKAFEELADSTVGLFGGAERVQVT
                     LHAPVSLAALWLAPKLPAFNRANPSIEVRLSSVIWDNAVLDEATDLEIRYGTGHWHGY
                     RSQRLLQQNIRAVCSPALLKRAGASAAAPDLLPRHLIHIIGYENHWLKVRQELDLASA
                     PPGAGPNVDTTIAALELAAAGEGIALTHPIFLAAYFTSGRLVSALAGEFADENSYFVI
                     TPERPQRIRREVQLLRDWLLAESAADGSENRLRRGIN"
     gene            5443..7860
                     /locus_tag="VGE92_03380"
     CDS             5443..7860
                     /locus_tag="VGE92_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1312888.1"
                     /translation="MKTNARVVVVGGGIMGASLLYHLALEGCTDTVLIEKGELTSGST
                     WHAAGQCPSITGSYNLAKIHAYSNQLYPKLEALSGQYVSWHPCGGLRLATNEHELAWL
                     RQVYGFSKSIGFRMEIVGLDVIKRLNPFLTTDHVMAAAWTQDDGHGDPSGLCNALAKA
                     ARDLGASVVRHNRVLEIRQRRGGEWQVLTEKGPIVAEMVVNAAGCYARQVAAMVGTDV
                     PITNMQHHYVVTHPIPAFMERSEEIPVMRDSYTSGYFRQEQKSGLMGIYESTGLTEAW
                     PPRGVPEWESTNELFADDLDRIAPWLERAIERMPIFGEVGIRRVINGAIPHTPDGAPL
                     LGPAAGLRNFWMCCGTSFGIAQGGGCGKYLAQWMLHGDAEINMTEFDPRRFGAFADEA
                     YVRDKVFQDYRLTFTTRRPGEEEMAGRPRKMSPLHDTLRAQGCVHSETFGWERPKWFS
                     LDGRSEDYSYRRNNVFDVVRDEVAAVHERVGVLDLTGFAKFDITGAGAEAFLNRVCAN
                     LVPKHAGGIGLMHLLSPAGRVQGEMTVTRLGDTRFYALSAAAAELRDEDLLKQSVTPG
                     EPVRIENVTEARGVLVLSGPCSRELLATLTDEDVSNESFRWLRAREMRIAGQPVRALR
                     VSYVGELGWELHAPIEALPALYEAVWRAGRRFGIANYGLYAVNSMRIEKGYKAWSSEL
                     TNELNMLEADMSRFINFEKSEFVGRTATLAQAPRPYRIVYTEVAATDTDARGGEPVLV
                     KDRCIGVVTSGAYGHRVAKSLAFACVAPEFAVAGSSFDVLIQGERRAANVLGAAAFDP
                     DNTRMRA"
     gene            7862..>8472
                     /locus_tag="VGE92_03385"
     CDS             7862..>8472
                     /locus_tag="VGE92_03385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1312889.1"
                     /translation="MAADLPTRARVVIIGGGVIGCSVAYHLTKLGWSDVVLLERKQLT
                     SGTTWHAAGLVGQLRQSINMTQLARYTAELYRGLEAETGQATGYRQCGSLSLATSAGR
                     MEELKRNASMAQVFGLTVKVVGPQEIHALYPLANLQDVIGGVHIPSDGYANAVDITQA
                     LAKGARARGALILQDLKVTAIQHDGRRVSGVQTDRGRIDADYVV"
BASE COUNT         1621 a         2649 c         2685 g         1517 t
ORIGIN      
        1 tgtcgcacca gttgcaaggc gagcgtgctg gtttgatcaa tcggcaattc gatacggctt
       61 ttctcgacgg cctgacccag aacccgctgg cgctcacccg aatatcacac accgaataca
      121 tgcgtgaggc gggttcggag ggtgtcgaga tggtgatgtg gcatgtcatg cgcggcgcgc
      181 tcgacgacga cgccgtcgag gtgtatcgcc attaccatgt tccggcctcc aatacggctg
      241 cgggcctgct gattctagag aataggagtt cgccatgaag gtctgtctcg cgggtcacgg
      301 cgcattcggc atcaaacacc tggaagcgat ggcgaagatt ccgggaatcg aagtgatctc
      361 gctcgcggga tcgaacctgg actcggcgcg cgaagtcgct cagcagtgga aaattccgca
      421 ttggaccgcg actctcgccg aaagtctggc gcagccgggt ctcgacgccg tgattttgac
      481 cacgcccacg cagatgcacg cggcgcaagg tgtcgaaagc atgcgcgcgg gaaagcacgt
      541 gatgatcgag attccgatgg ccgactccgt cgctgactcc gaactgctgg tgcgcgtgca
      601 gaaagaaacc ggcgtggttg ccatggccgg gcacacgcgc cgcttcaatc ccagccatca
      661 atgggttcac aagcgcattc tcgcgggcga actcaaggtg ctgcagatgg acgtgcagac
      721 atattttttt cgccgcacca acaccaacgc gctcggcaag ccgcgcagct ggacggatca
      781 tttgctgtgg caccatgcct gtcacaccgt ggacttattc ggctatcaga ccggcgagcg
      841 gatctcgcag gaatttgcgc tgcaaggccc gaaacacccg gtgctcggaa tcgcgatgga
      901 catgagtatc ggcctcaaag tcccgtcggg cgccttgtgc actttgtcgc tgtcattcaa
      961 caatgacggg ccgctcggca ccttctttcg ctacatttgc gataatggca cctacatcgc
     1021 ccgctacgac gaactgctgg acggcaagaa caatccgatc gacgtgtcga aggtggatgt
     1081 ttcgatgaac ggcattgaat tgcaggatcg ggagttcttt gccgcgattc gcgaccagcg
     1141 cgagccgaat tccagcgtcg cgcaagtact gccggcgatg ctcacgctcg ggcgcctcga
     1201 gcagcagctc ggcaagtgag cgtccgcgcg ggcgcttgtt cagtcttcga acggcaaccc
     1261 cacatagttc tcggcgagac tcgcgagcgc cgctgtcgag ccggtcaggt agtccagctc
     1321 cgccagctgc aaccgatgcg cgaacgcatc atcggagaat ttgtgcgtaa ccgaggtgaa
     1381 ccaccatgaa aagcgcaccg ccttccaaat gcgccgcaga caccgtgccg agtagcggtc
     1441 gatgagagcg gcgctgcccg ttcgatagta ttccaccagt gcctgataca gcacccgcac
     1501 atccgcggcg gcaaggttca agcccttggc gccggtaggc ggaacaatgt gcgcggcgtc
     1561 gcccaccaag aatagctgac cgaacttcaa gggttcgacc acgaaacttc gcaacggcgc
     1621 gatgctcttt tcgatcgagg gtccggtcac gatggcatcg gcgacctgga ccggtagacg
     1681 cgagcgcagt tcctcccaga agcgcgcgtc ggaccatgcc tccactttct catcgaggcc
     1741 gcattgcacg tagtagcggc tgcggttgag cgaccgcatg ctgcatagcg cgaagccgcg
     1801 ctcgtgattc gaatacacca attcgtgcgc cgccggcggc acgtcggcga gaattcccag
     1861 ccagccgaac ggataaattc gctcgaaact ttgcagcgcg gtctgcggta cgctgcggcg
     1921 gcaaacgccg tggtagccgt cgcagccggc gacgaaatcg cagctcagtt cgtgggttcg
     1981 gccgtcctgg acgtagcgca cggtgggacg tgcgcggtcg aagccttcga tcgatacctc
     2041 gccggcttcg tagatcatgg tcgcgcccgc ggctgcgcgc gcgtccatca ggtccttggt
     2101 cacctcggtt tggccgtaaa ccgacacggc attgccggcg aaacgtttca agtcgacgcg
     2161 aaaggtttga cccgcgaatc cgagcgcaaa gccatcgtgt accagccgct cgcgcagcat
     2221 gcgctcgccg acctgcgcct gcagcagcag ctcaacggta ccctgctcca gtacgccggc
     2281 gcgaatccgt ccggcgacgt attcgcggct ctgccgctcc agcaccacgt tgtcgacacc
     2341 atgcaggtag agcagctgcg acagcagcag cccggacgga ccggcgccga tgattgcgac
     2401 ttgagtgcgg gccatcataa aagcctagca gcaccgctgc tgcccgattg cacgattaga
     2461 ttgcgcgctg gtacttatcc gccatggtga cgccggcgaa aattcagcgg gctcgagccg
     2521 ttgtggcggc gaaaaaatcg gcagaagtac gccgggtcct tgaatcccag ttccgcggcg
     2581 atgccgctta ccgagctgcc tgcatacacc aggcgtcgcc gcgcctcgag ggccaggcgc
     2641 tgttggatca gatcgaaggc ggtggcgccc gccaagcgcc ggcagaggcg attgaggctg
     2701 gtctccgaca atgccagcga gcgcgcatag cgcgccaccg gccaatgctt gaggtactgc
     2761 gattcgatca gttgacggaa gcgccgcagc cgcccggcat cgagcccgct cggaagttcc
     2821 gtggcgtcgc cggcggcgca gccctgcgcc aatatccaca ataggcaaca cgcgagccat
     2881 ccgccgatcg gcccggccca gctgtcgggc tgccggaatt cttgctcgag cagttccaag
     2941 agctgctcga gccgctgcgc tagcaacggt tcgtcctgca gatcgatggc gcgggcgatc
     3001 gcgaacagag tctcgaccgg tgagcgctgc aacggcgttg ccgcgctcaa aacctgcgcc
     3061 agatcgatcg tcagcacatg cccctgggtg tcggcgcgaa accgaaaact gtgcacggtt
     3121 ccggcgggta tgatcatgac ggccggcccc gccaaagcgg cgcgactgtc gtccagggtc
     3181 acgatcaccg gacctgaagt caccaccacg cattggctca gcaagctgtg tcgatgagtg
     3241 ccgattcgcc acaggtactt gcggctgcgc gattgaatgt cttcgatgtg aatgccgtcg
     3301 gtttgcgccg cgcgcggcgt ttcgccgtac agcgagaagc tcgggatggc gcgtcgcctg
     3361 gcgcgcacgg cgttactcgg agacgagcgc cacgtcgctc accagcagca cttcgcaggc
     3421 gcccgctccg gtgtcggtgc gcaccagcga ggtgttgatc caagttccgt cgtcgcgcac
     3481 gccgtcgacc aaatcaccgg acagggtcac gatctcgccc ggccgcagcc gcgacagctg
     3541 ccgcgcaatg gatacatcgc gcgggatcac gtgcgtgttg gcactgtgga caatgatcga
     3601 gtccttgggt atgggcgcat tgcggcgtgc gcgccaagag tagaaacgat tcgactgcgt
     3661 gatctcgatg ccgcgcagaa tccgactgtc cgacatcgga ccccagccga gcgcgagatc
     3721 ctccgggatg aggtcggcga gcgcatcaaa atgatagcgc tcgaggccta gaatgcgcgc
     3781 tgtgatccta tagtgagcgc gcaccgtcaa gcgccatcgc ccaatctgca atttttcacc
     3841 gccgggtgcc tcggtttgcc gcggatcgtc ggcggcaagc acgccatccg gcgggtggac
     3901 cggccgattg atgaaccaat tccacccgcc ccagacaacg tagaccagga agagtattgc
     3961 gagaagaggg tggcgccgca gttaggcggg cttcgctgcc cacaccttgc accagcccgc
     4021 cgcgggtacg ctcttgccgg caaaaatatt gcaaccggct gtggcatcgc tcgccttgcc
     4081 ttggtattgc gcgcacgtgc tgcacttctg tccggccttg tacgttgcgt tggcggcggc
     4141 gtccaccttg gtcgcatcgg tcacgaaacc caaggcttgc gccgtgggat ccttcggatc
     4201 aagcgccgtc agacctgcgg cctgcgagga ctgggtcatc agacccatgg ccggaatcat
     4261 ggcgcccgcg atcagtccgc cctttacgac agctcgacgt gaaatcctgc tttgcattgg
     4321 tgtgatccta tggtgaaaga ctcgcgagca ttttcccgct tttcgactcg cgtatgtcat
     4381 aaatattaat taatgccgcg gcgcaaacga ttctcactac catctgccgc cgactctgcg
     4441 agcaaccagt cacgcagcag ctgcacttcg cggcgtatgc gctgcggccg ttccggggta
     4501 atcacgaagt aggagttctc gtccgcaaat tctccggcaa gcgcgctcac cagccgcccg
     4561 ctcgtgaagt acgccgccag gaaaattgga tgcgtgagtg caatcccctc gccggccgcc
     4621 gcgagttcga gtgccgctat cgtggtgtcg acgttcgggc cggcgcccgg cggggcactc
     4681 gcgaggtcaa gctcctgccg aaccttcagc cagtgatttt cataaccgat gatgtgaatc
     4741 aaatggcgcg gcaacaggtc cggcgcagcg gcgcttgcgc ccgccctttt caacagcgcc
     4801 ggactgcata ccgcccgaat attctgctgc aggagccgct gcgaccggta gccgtgccag
     4861 tgacctgtgc cgtaacgaat ttccaaatcc gtggcttcat cgagtaccgc gttgtcccag
     4921 atcaccgacg atagccgcac ctcgatcgat gggttggcac gattgaaggc gggcagcttc
     4981 ggcgccagcc acagcgcggc gagcgacacc ggagcgtgca gcgtgacttg cacccgctcg
     5041 gcaccgccga aaagtcccac ggtcgagtcg gcgagttctt cgaaggcctt gcgcaccggc
     5101 ggtagatagg ccacccccat tggggtcaac ttgacgccgc gcggcaggcg ctcgaataag
     5161 gcgaatccca agtgagcctc gagcccgcgg atttggtagc tgatggcgcc cgaggtcaaa
     5221 ttcaattccg ctgccgccgc cgcaaagctg gaggtgcgcg ctgcggcctc gaaggcccgc
     5281 agccaggtga gggttggcag tccaacgacc gggcgaattt gactcaatgc aatcctcggg
     5341 gctaaaaatt taacgccaaa ggtagcaaat tcttaagttg tatgccccgt tccctcaatt
     5401 tattgtggtc aactaccctc gccgatcgca acaaaggctg gcatgaagac aaacgcacgc
     5461 gtggtggtgg tcggcggcgg catcatgggg gcgagcctcc tgtatcacct ggcattggag
     5521 ggctgcaccg ataccgtcct gatcgagaaa ggcgagctga cgtcgggctc cacctggcac
     5581 gccgccggtc aatgcccgag cataaccggc agctataacc tggcgaagat tcacgcctac
     5641 agcaaccagc tgtatccgaa actcgaggcg ctgagcggtc agtacgtcag ctggcatcca
     5701 tgcggcggcc tgcggctcgc gaccaacgag cacgaactcg cgtggcttcg ccaggtgtac
     5761 gggttttcga agagcatcgg ctttcgcatg gaaatcgtcg ggctcgacgt gatcaaacgc
     5821 cttaatccct ttctgacgac cgaccacgtg atggcggcgg cgtggaccca ggacgacgga
     5881 catggtgacc cgtcaggtct gtgcaacgcc ctggccaagg ccgcgcgcga cctgggggcg
     5941 agtgtggtgc gccataatcg cgtgctcgag atccgacagc ggcgcggcgg cgaatggcaa
     6001 gtcctcaccg aaaaagggcc catcgttgcc gagatggtcg tgaatgccgc cggctgctat
     6061 gcgcggcagg tggccgccat ggtgggcacc gatgtgccta tcaccaacat gcagcaccac
     6121 tacgtcgtga ctcatccgat ccccgcgttc atggagcgca gcgaagaaat tccggtcatg
     6181 cgggattcct acacctcggg ctactttcgt caggagcaga aatccggctt gatgggtatt
     6241 tacgagagta ccgggcttac cgaggcatgg cctccgcgcg gggtcccgga atgggaatcc
     6301 acgaacgaat tgtttgccga cgacctggac cgaatcgcgc cgtggctcga gcgcgccatc
     6361 gagcggatgc ccattttcgg tgaggtcggg attcggcgcg tcatcaacgg cgccataccg
     6421 catacgcccg acggcgcgcc gctactcgga cccgcggcgg gcctgcgcaa cttttggatg
     6481 tgttgcggaa cttcattcgg catcgcgcaa ggcggcggct gcggcaaata cttggcgcaa
     6541 tggatgttgc atggcgatgc cgagatcaac atgacggagt tcgacccgcg gcgcttcggc
     6601 gcctttgccg atgaggcgta cgtgcgcgac aaggtgttcc aggattatcg attgaccttc
     6661 accacgcgcc gcccgggcga ggaagagatg gccggacggc cgcgcaaaat gagcccgctg
     6721 cacgacacgc tgcgtgcgca aggctgcgtg cactcggaaa cgttcggctg ggaacggccg
     6781 aagtggtttt cgctcgacgg ccgcagcgaa gactacagtt accggcgcaa caacgtgttc
     6841 gacgtggtgc gcgatgaagt cgccgccgtg catgaacggg tcggcgtact cgatctcacc
     6901 ggctttgcca agttcgacat cacgggcgcc ggcgccgagg ccttcctgaa tcgagtctgc
     6961 gcgaatctcg tgccgaagca tgcgggcggc atcgggctga tgcatttatt gtcgcctgcc
     7021 ggccgcgtgc aaggggaaat gacggtcacc cggctgggcg atacgcgctt ttacgcgttg
     7081 tcggcggccg ccgcggaatt acgcgatgag gatctgctca agcaaagcgt cacacccggc
     7141 gagccggtgc gcatcgagaa tgtcaccgaa gctcgcggcg tgctggtgtt gagcggaccg
     7201 tgctcccgcg agttgctcgc gacgctgacc gacgaggacg tgagcaacga gtcgtttcgc
     7261 tggctgcgcg cgcgtgagat gcgcatcgcc gggcagccgg tgcgcgcgct gcgggtgagc
     7321 tacgtcggcg agctcggctg ggaattgcat gcgccgatcg aggcactgcc ggcgctgtac
     7381 gaagccgttt ggcgtgccgg acgcaggttc ggcattgcca actacgggct ctacgccgtc
     7441 aattccatgc gcattgagaa gggctacaaa gcttggtcga gcgaactgac gaacgagttg
     7501 aacatgctgg aagcggatat gtcgcgcttc atcaatttcg agaagagcga gtttgtcggc
     7561 aggaccgcga cgctcgccca ggcgccgcgg ccgtacagga tcgtttatac ggaagtcgcc
     7621 gcgacggaca cggatgcgcg cggcggtgag cctgtgcttg tcaaggatcg gtgcatcggc
     7681 gtcgtgacct cgggagccta cggtcaccgc gtcgccaaga gccttgcgtt cgcctgcgta
     7741 gcacccgaat ttgccgtggc cggcagcagc ttcgacgtac tgatccaagg cgagcggcgc
     7801 gcggccaacg tgctcggagc cgccgccttc gatccggaca atactcggat gagggcttaa
     7861 cgtggccgcg gacctgccca cgcgggcgcg cgtcgtgatc atcggcggcg gcgtcatcgg
     7921 ctgctcggtg gcctatcacc tgaccaagct cggctggagc gatgtggttc tgctcgagcg
     7981 caagcaactg accagcggca ccacctggca tgccgcgggg ctcgtaggcc agctgcgtca
     8041 gagcatcaac atgacacagc tcgcccgcta cacggcagag ttgtatcgcg ggctcgaagc
     8101 tgagaccggc caggcaacgg gctatcgtca gtgcggctcg ctgtcactgg cgaccagcgc
     8161 cgggcgcatg gaggaattga agcgcaacgc ctcgatggcg caggtctttg gactgacggt
     8221 caaggtcgtc ggcccgcagg aaattcacgc gctctacccg ctcgccaatc tgcaggatgt
     8281 gatcggcggc gttcacatcc cgagcgacgg ttacgccaac gccgtcgata tcacacaagc
     8341 gctcgccaaa ggcgcacgcg ctcgcggcgc gctgattctg caggacctca aagtcaccgc
     8401 catccagcac gacgggcgtc gcgtcagcgg cgtacagacg gatcgaggtc gcatcgacgc
     8461 cgactatgtg gt
//
LOCUS       DASWZT010000124        12283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_25101, whole genome shotgun sequence.
ACCESSION   DASWZT010000124 DASWZT010000000
VERSION     DASWZT010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12283
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25101"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VGE92_03390"
     CDS             complement(<1..665)
                     /locus_tag="VGE92_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY1312890.1"
                     /translation="MLYGLAGLALAVLLRKLALRLQLSRAKHRSLSGHSRMARRFASL
                     VPFYEYDEAHFFRADAAPPDVAAGRRAGFMRLAAVFAERFKKTLELTGEVADAVSDLQ
                     FTSRYRVPFQFSGFVRRHLNSGSFIESSCGVTITDLDGNRFYDLTGSYGVNVFGYDFY
                     KECIEQGSRHVQGLGPVLGAYHPVTAYNVRSLLQISRQEEISFHMSGTEAVMQAVRLA
                     RYHT"
     gene            738..1703
                     /gene="asd"
                     /locus_tag="VGE92_03395"
     CDS             738..1703
                     /gene="asd"
                     /locus_tag="VGE92_03395"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HEY1312891.1"
                     /translation="MTAKSLIARIVQQEDINFLLTNRIPRRLLTQFTGWLSRIEQPWV
                     SAACIALWRLFSDLDLSEAKQTNFKSVHACFTRELKAGARPVDSDPRCLTSPCDALVG
                     ACGTVTDGSVLQAKGFPYPLLDLLADPELVGYYRDGQYATLRLTSSMYHRFHAPADCR
                     VEQVTYISGDTWNVNPIALKRVEKLFCKNERAVIRCRLAASGALLTLVPVAAILVASI
                     RLRFLDVLLRLSYRGPNVIPCDAALHRGEEMGWFEHGSTIIVFAPRGARLSDSVHEGG
                     RIKAGQALMRIDRIQAVPPVAAAALRNSVCTAVSPSTAAALRLAE"
     gene            complement(1639..2322)
                     /locus_tag="VGE92_03400"
     CDS             complement(1639..2322)
                     /locus_tag="VGE92_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1312892.1"
                     /translation="MVTPEGRLYETLPDLPVTDITQLRSDTLWSYEAGTKLQLPNPGL
                     LLSAAAFHIDWKNIQQLGAEIELAGHLSRALQVRFGAGYEKTKITAPGALSIVGVDPG
                     SRILGTPAWTATIGAVYTRTLANNLDGFISADYSYTGDSVSLLNGGYGAEATRPAYSL
                     ANLRFGIDREQSEVSLNIHNLSNAKPNLGDIGYVGYAQYQNYAAGTIIPQVATLQPLT
                     VILQYKQSF"
     gene            complement(2418..3386)
                     /locus_tag="VGE92_03405"
     CDS             complement(2418..3386)
                     /locus_tag="VGE92_03405"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEY1312893.1"
                     /translation="MRSQQDSAGGLRHLVTLEGLSREALVAILDRAETYRRLPGQPAY
                     EGRELAGVTLANLFFEPSTRTRASFDLASRRLGAHVLNLDLHSSSRAKGESMLDTIYT
                     LEAMNTDVFVIRDSEAGMPEFLIDHVAPHVSVISAGEAHVSHPTQGLLDVLTIRTHKR
                     RFAGLKVAIVGDIKHSRVARSVHQALSTLGTTEVRMVAPEALLPAEGEFRGALRFTRI
                     EDGIAGADVVMMLRIQRERMVQSASLDDAAYFRDFGLDAKRLKLASPSALVMHPGPMN
                     RGVEIASEVADGARSVIREQVNNGVAVRMAVLAAIAATLTRHRRPL"
     gene            complement(3394..3855)
                     /gene="ruvX"
                     /locus_tag="VGE92_03410"
     CDS             complement(3394..3855)
                     /gene="ruvX"
                     /locus_tag="VGE92_03410"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEY1312894.1"
                     /translation="MPEATGPVPVPGAPLIVLAFDFGLRRIGVACGDTLSRAASPLQA
                     VASDAAGPRWGMIDALLREWQPSLAVVGLPYNVDGSDSAMTSAAREFAAEMGRRYPLK
                     VVLVDERYSSQEAQALLKSLRESGLRRRRVGKEDIDAAAACVILERWFGQH"
     gene            complement(3848..4369)
                     /locus_tag="VGE92_03415"
     CDS             complement(3848..4369)
                     /locus_tag="VGE92_03415"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY1312895.1"
                     /translation="MPSMGDPNFAQTVALVCDHSPSGALGLILNKPLPMRMAEIFDQL
                     EIELNLGPLRERPVLRGGPMQTDRGFVVHRAGGEWDSTLKVSEVIHVTTSRDILAAMA
                     RGEGPQEAVVALGYAGWDSGQLEDEIRANAWLNAPVDPGLIFDLPFESRWHAAGRLLG
                     VELSRISPISGNA"
     gene            complement(4407..5336)
                     /locus_tag="VGE92_03420"
     CDS             complement(4407..5336)
                     /locus_tag="VGE92_03420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY1312896.1"
                     /translation="MTTATYQPADMLHSGLPPASDRMLTTCFLAALFHAIVILGVTFS
                     SPGSKAADDGAPSLEVVLVNDQVPSVAKNPRAQYLSERTQVGSGNSIRRERTLIPKSS
                     LMPVERSGIPSGAGLAAQQAGSDAGNEELVATRTRAQNIFYFASAASAKESSQLPVLL
                     EKRPDLAITPNDDGVELHLRGEPSHQLWIAADTRESDVAVYLDSWRRKIERVGTMNFP
                     DAARRQKLSGTPVIEVTIGADGKLLQTLVRRSSGHAEIDEAAMRILKLAAPFDPFPGD
                     LVAKHDEIRIAYEWQFLGGASQGSHVFYSEPSN"
     gene            complement(5333..6295)
                     /gene="gshB"
                     /locus_tag="VGE92_03425"
     CDS             complement(5333..6295)
                     /gene="gshB"
                     /locus_tag="VGE92_03425"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003195061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEY1312897.1"
                     /translation="MSSSARLVVVMDPIEAIKPAKDSTLAILLAAQARGWQLFYAEQK
                     HLWLRDGVAWGRLAPLQVFKDLEKWFVRGATRDAKLGEFDVILMRKDPPFDMEYIYTT
                     YILDRAEGQGALVCNRPQGLRDMNEKVYTAWFPECCAPTLITRDMHDMSEFLREHGKA
                     VCKPLDGMGGKSIFVLEHGDKNRNVVFESLTAYGSRYAIVQRYLPEIVTAGDCRILLV
                     DGEPVPFALARIPHADDNRGNLAAGARGESRALNPRDRWLCSQIGPKLKAAGMIFVGL
                     DVIGDFVTEINVTSPTGIRELNKMQGVDIGALLVDALQRTLGSA"
     gene            6532..6936
                     /locus_tag="VGE92_03430"
     CDS             6532..6936
                     /locus_tag="VGE92_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014720321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1312898.1"
                     /translation="MESTITNGKPKLTGLKVLVIDDSKTIRRTAETLLTKEGCEVFTA
                     VDGFDALSKIADHQPDIVFVDIMMPRLDGYQTCSLIKHNKVFKQIPVIMLSSKDGLFD
                     RARGRIVGSEHYLTKPFTKDELLGAIEAHVGK"
     gene            6951..7316
                     /locus_tag="VGE92_03435"
     CDS             6951..7316
                     /locus_tag="VGE92_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1312899.1"
                     /translation="MALILIVDDSPTEVFVMQKALEKHGFKTAAAENGEEGVRKAKAM
                     KPDLIFMDIVMPGVNGYQATRMLINDPETSLIPIIMVTTKGQETDRVWGLRQGAVDYL
                     VKPVSAQALVEKAQAALAV"
     gene            7313..7888
                     /locus_tag="VGE92_03440"
     CDS             7313..7888
                     /locus_tag="VGE92_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY1312900.1"
                     /translation="MTSMSGTENSLRSLRDRPFELLKELEKRSRAVSASSVPEASAGQ
                     EWVGVAFRMGGETFLVAREETREVLGYPAVVTRIPGAKSWVKGLANVRGQLLPMLDLR
                     QFLGSGATASSRNTRVVVVNHRDIPAGLMVDEVLGFRRFAEAEFNAEAPPTVIRCDSY
                     LAGAFRRGGEVWPVLSLKSLVESQSFLQAAS"
     gene            7906..9984
                     /locus_tag="VGE92_03445"
     CDS             7906..9984
                     /locus_tag="VGE92_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY1312901.1"
                     /translation="MTTRNDTGPGPNFLPVLAALIVLCLGSAAGLTLLTHNAAPVAAP
                     AELSLVVQRIPFEAQNALRGESSAFDALAKSAARLKTLRAAAADTAPASAGNWNKMTD
                     DVSAVSEARAAVATIQGSSQEARELAPKLLSELGDLASAVGVQKLEGMSRYLERFELM
                     VQRIQQDLNGLATGVSDAAPAAQRLADSLEFLNQVMRGLSGEESGLALTRVTGADAEQ
                     RLKTVVARNQQYGTSVRKAIGAAEALSKAQTAARTLPAVAGSLATQLGDVPARTDAGA
                     SYKPWILSLLGAAALLLAGLAWIYLKTVNVRRSSELRVRENERNQEAIMRLLDELSSL
                     ADGDLTVQATVTEDITGAIADSINYAIEALRELVTTINDSAIQLDGATKQTQAAASHM
                     AKASSAQSRQISSASESMVDMAASIEEVSGNSERCSDVARHSVDIAHKGGDAVRRTID
                     GMNAIRETIQETSKRIKRLGESSQEIGNIVELINDIAEQTNILALNASIQASMAGEAG
                     RGFAVVADEVQRLAERAANATKQIEVLVRTIQTDTNEAVVSMERSTTDVVGGALLAEN
                     AGAALEEIEQVSNQIASLVQNISASARQQAVASGNISKNMQVVREISTQAAAGTTATS
                     TSIAKLAALSAQLRKSVAGFRLPDYGGGTTTIGANSASASSPRLPPVNEPANNGGSGA
                     KIRKASGFGA"
     gene            10003..>12283
                     /locus_tag="VGE92_03450"
     CDS             10003..>12283
                     /locus_tag="VGE92_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY1312902.1"
                     /translation="MTDISSQTLHIVAKELAVTLNDARASLEAYAERPDQQELLEKCA
                     DQLHSAHGVLRLVEVYGAALLAEEMEHVTRYLLSSDNDQKRQVDGLDALMRAMVQLPT
                     YLERVMSGGRDLALVLLPLLNDLRSVRGSPLLSEGTLLLLNLKSDQPAQPQAQRQGEQ
                     IISVAQWARRLRPRFQIGLLGYIRGERTEQNLEILAKVADKLEQVATSQPVFQLWWVV
                     GAILEAVRANGLEGSATLKRLLGQADRQIKTLYELGEARYCEDPPIDLLNNLLYYVAR
                     STSGGGRIAAVRASFKLTELLPVDDSIEQERESLSAPSVKLMRTVAAAIKEDLSKVKD
                     VLDIFVRRGGGRSEELIPQLELLKKISDTLGVLGLGELRQRVQEEISELSDIVTDGKI
                     PPDESLIKVAGVLLSVEDSLDDQLVRLILPATSERPLTEVSGDQDLEYKLVSEAVLRE
                     CIVNLARIKEAVSLAVHKPGDAAPQGLDNVPQLLRGITAGLLMLGKGRAVELMDAIGT
                     EVRKLIEPGAPPPEPIRLERVADAIVSIEYYMETLQSGRTDPWYMLDNAETCLKTLEE
                     QAPSRVPNISISSSEAAKTVKIDPIQTVQLERAKRTHAATHPLIVVPEPEPVDPQFLE
                     LFIEEAKEEIASIQNSFPLWDQNPMDLESLALMRRSFHTLKGSGRMVGARSIAEFGWS
                     IENLLNRITDKTLSRTPGMMALLRSSIAALPQLVEHLETGRQNTVPIEALMARAFAYA
                     DGREADQGTITLTPADREMA"
BASE COUNT         2424 a         3757 c         3920 g         2182 t
ORIGIN      
        1 gtgtggtagc gggcgaggcg cacggcctgc attaccgcct cggtgccgga catgtgaaag
       61 gagatctcct cctgccgcga aatttgcagc aggctgcgca cattgtaggc ggtcaccgga
      121 tggtacgcgc cgagcaccgg ccccaagccc tgaacatgcc gactgccttg ttcgatgcac
      181 tccttgtaga aatcgtagcc gaagacgtta acgccgtacg agccggtcaa atcgtagaac
      241 cgattgccgt cgagatcggt gatcgtcact ccgcaggagg actcgatgaa gctgccggag
      301 ttgaggtgac gccgtacgaa gccgctgaat tggaacggca cgcggtagcg ggacgtgaac
      361 tgcaggtccg acactgcgtc ggcgacctcg ccggtgagct cgagcgtctt cttgaagcgc
      421 tcggcaaata cggccgccaa gcgcatgaag ccggcacggc gcccggccgc gacgtcgggc
      481 ggggcggcat cggcgcggaa gaaatgcgcc tcatcgtatt cgtaaaacgg caccagcgag
      541 gcgaagcgtc gcgccatgcg tgaatgcccg ctcagcgagc gatgcttggc gcgcgacagc
      601 tgcagccgca gcgcgagctt gcgcagcagg acggcgagtg cgagtccggc caacccatac
      661 aacatcagcg tagcaattgc cgttttccgt aaattacccg tagccagcgc actgtattgc
      721 agggcgatga cactgagatg acggcaaaat ccctgatcgc gcgcatcgtt cagcaggaag
      781 acatcaactt cctgttgacc aaccgcatcc cgcgtcgttt gttgacgcag ttcacaggct
      841 ggctcagccg aatcgagcaa ccgtgggtgt cggccgcctg catagcactg tggcggctat
      901 tctcggatct cgatctgagc gaggcgaagc agacgaattt caagagcgtg cacgcctgtt
      961 ttacgcgcga attgaaggcc ggtgccaggc cggtcgattc ggatccgcgc tgcctgacga
     1021 gcccatgcga cgcgctggtg ggcgcttgcg gcacggtaac ggacggatca gttctgcagg
     1081 cgaagggttt cccgtacccg ctgctcgatc tgctggcaga cccggagctg gtgggttact
     1141 accgggatgg ccaatacgcg accttgcgcc tgacgtcgag catgtatcac cgctttcacg
     1201 cgcctgccga ctgccgagtc gaacaagtga cctacatatc gggcgacacg tggaacgtga
     1261 atccgattgc gctcaagcgg gtcgagaaac tgttctgtaa gaacgaacgt gcggtcatcc
     1321 gctgccggct cgccgccagc ggagcgctgc tgacgctcgt gcccgtggcg gcgattctgg
     1381 tcgcaagtat ccgcctgcga ttcctcgacg tgctgctgcg tctctcctat cgcgggccca
     1441 atgtcattcc ttgcgatgcg gctctgcatc gcggcgaaga aatgggttgg ttcgagcatg
     1501 gctcgaccat catcgttttc gcgccgcggg gagcccgctt gagtgacagc gtgcatgagg
     1561 gcgggcgcat caaggcggga caggccttga tgcggatcga ccgcatccag gccgtgccgc
     1621 cggttgccgc ggctgccctt agaaactctg tttgtactgc agtatcaccg tcaacggctg
     1681 cagcgttgcg acttgcggaa tgatggtccc ggccgcgtag ttctgatact gcgcgtatcc
     1741 cacatagccg atgtcgccga gattcggctt ggcgttgctc aggttgtgaa tgttgagcga
     1801 cacctccgat tgctcgcgat cgatgccgaa gcgcagattg gccagggaat aagcggggcg
     1861 cgtcgcctcc gcaccgtagc cgccgttcag cagcgagacg ctatcgccgg tatagctata
     1921 atccgcggag atgaagccgt cgaggttgtt ggcgagggtg cgcgtataca ccgcgccgat
     1981 cgtcgcagtc caggcggggg tccccaagat gcgcgagccc ggatcgacgc cgacgatgct
     2041 gagcgcaccc ggtgcggtaa tcttggtctt ttcgtagccg gcaccgaagc gcacttgcaa
     2101 agctctcgac aggtgccctg caagttcgat ttcagcgccg agctgctgga tattcttcca
     2161 atcgatgtga aacgctgccg ccgacaagag caggcccggg ttcggcagct gtagcttcgt
     2221 gcccgcctcg taactccaca gggtgtccga gcgcaactgc gtgatatccg tgacgggcag
     2281 gtccggcaac gtttcataca gcctcccttc aggcgtgacc acgatttgtt atattatttt
     2341 gataataata tgaaagagcg gaactcggca acggctgcga tgcgcgccgg cggcgagcag
     2401 ggcgagggga tccgcggcta gagcggccgg cgatggcggg tgagtgtggc cgcaatcgcg
     2461 gccagcaccg ccatgcggac ggcgacaccg ttgttgacct gctcacgaat gaccgagcgc
     2521 gctccgtccg ccacctccga tgcgatttcc acgccccggt tcatcggacc cggatgcatg
     2581 acgagcgcgc tgggcgaagc caatttcagg cgtttggcgt ccagtccgaa atcccggaag
     2641 taggccgcgt catccagcga cgcgctttgc accatgcgtt ccctctggat gcgcaacatc
     2701 atcaccacgt cggcgcccgc aataccgtct tcaatgcgag tgaagcgcag tgcgccgcgg
     2761 aattcgccct ccgccggcaa cagcgcctcg ggcgccacca tccggacttc cgtggtgccg
     2821 agcgtgctga gcgcctgatg gaccgatctc gcgacgcgcg aatgcttgat gtcgccgacg
     2881 atcgccacct tgaggccggc aaagcgccgc ttgtgggttc gaatggtcaa tacgtcgagc
     2941 aatccttgcg tcgggtgaga tacgtgcgcc tcaccagcgc tgatcaccga gacgtgcggt
     3001 gcgacgtgat cgatgaggaa ttccggcatg ccagcctcgg aatcgcggat cacgaacaca
     3061 tcggtgttca tggcttcgag cgtataaatg gtgtccaaca tgctctcgcc cttggcgcga
     3121 gaactcgaat gcaagtcgag attgagcacg tgagcgccca aacgacggct cgcgagatcg
     3181 aacgaggcgc gcgtgcgggt gctcggttcg aaaaaaaggt tggccagcgt aacgccggcg
     3241 agctcgcggc cttcgtaggc cggctgtccc ggaagccggc ggtaggtctc ggcgcggtcg
     3301 agaatggcga ccagcgcctc gcggctcagc ccctcgagcg tgacgaggtg gcgcaggccc
     3361 ccggcgctgt cttgttgtga acgcatatgc gtctcaatgt tgcccgaacc aacgttccaa
     3421 tatgacgcaa gcagcagcgg cgtcgatatc ctctttgccg acacggcgcc ggcgcaggcc
     3481 cgactcgcgt agcgacttga gcagcgcctg tgcctcctga gacgaataac gctcgtcgac
     3541 cagcacgacc ttaagcggat agcggcgccc catctcggcg gcaaactccc gggcggcact
     3601 cgtcatggcg ctgtcggaac catcgacatt atacgggagg ccgaccaccg ccagggacgg
     3661 ctgccattcg cgcagcagcg catcgatcat gccccaccga ggtcccgccg catccgatgc
     3721 cacggcctgc aggggcgagg ccgcgcgact cagcgtgtcg ccgcaggcga caccgatacg
     3781 ccgcaggccg aaatcgaagg ccagcacgat cagcggcgcg cccggcacgg gcaccggacc
     3841 cgtcgcttca ggcattgccg ctgatggggc tgatacggga caattcgact cccaacagcc
     3901 gtccggctgc gtgccaacgg gattcgaacg gcaaatcgaa gatcaagccc gggtctaccg
     3961 gcgcgttcaa ccaggcgttg gcccggatct cgtcttccaa ctggccgctg tcccagcctg
     4021 cataacccag cgcgacgacc gcctcctggg gtccctcgcc acgggccatc gccgccagga
     4081 tgtcgcgcga cgtcgtgacg tggatgactt ccgacacttt gagcgtcgaa tcccattcgc
     4141 cgccggcgcg gtgcaccacg aagccgcgat cggtctgcat ggggcccccg cgcaacaccg
     4201 gccgctcgcg cagcggcccc aagttaagtt cgatctccag ctgatcgaag atttcggcca
     4261 tgcgcatggg taacggctta ttcagaatca gcccgagcgc cccactcgga ctgtgatcgc
     4321 aaaccaaggc cacggtttgc gcaaagttgg gatcgcccat cgagggcatg gcgatcagta
     4381 actgattggt gaggtaaccg gaatcgctaa ttgctcggct ccgaataaaa gacgtgactg
     4441 ccctgcgagg cccccccgag gaactgccat tcgtaggcga tgcggatctc gtcgtgtttc
     4501 gccaccaagt ccccgggaaa tggatcgaac ggtgcggcga gcttcaagat ccgcatggcg
     4561 gcctcgtcga tctccgcatg cccgctgctg cgccgaacca aggtctgcag cagtttacca
     4621 tccgcgccga tggtcacctc gataaccggc gtcccggaga gtttctggcg gcgtgccgca
     4681 tccgggaaat tcatggtgcc cacgcgctcg atcttacgtc gccagctgtc caagtaaacg
     4741 gcgacatcgg attcgcgggt gtccgcggcg atccacagct ggtgcgaagg ctcgccgcgc
     4801 agatgcagct ccacgccgtc gtcattcggt gtgatcgcga gatccggccg tttctcaagc
     4861 aacacaggca gttgcgatga ttctttggca gaggccgccg acgcaaagta gaagatattc
     4921 tgcgcacgtg tgcgcgtcgc gaccagctct tcgttgccgg catcggagcc ggcttgctgc
     4981 gccgcgagcc cggcaccgct tgggatgccc gaacgctcga ccggcatcaa tgacgatttg
     5041 gggatcaagg tgcgttcgcg gcgaatcgaa ttgccggatc ccacctgcgt gcgctcggag
     5101 aggtactgtg cgcgggggtt cttggccacg ctcggcactt ggtcgttgac caatacgacc
     5161 tccagggacg gcgccccgtc gtcagccgct ttgctccccg gcgagctgaa ggtcacgccg
     5221 aggatcacga tcgcgtgaaa caacgctgcc aaaaagcacg tggtcagcat ccggtcgctc
     5281 gcgggcggca ggccggagtg cagcatgtcg gctggttgat acgtagcggt cgtcacgcgc
     5341 tgcccaaggt ccgctgcaac gcgtccacca gcagcgcccc tatgtcgaca ccctgcatct
     5401 tgtttagttc gcgaatgccg gtgggactcg tgacgttgat ttcggtgaca aaatcgccga
     5461 tgacgtccaa gccgacgaag atcatcccgg ccgccttcaa tttgggtccg atctgcgaac
     5521 acagccagcg gtcccgtggg ttgagcgcgc ggctctcgcc gcgtgcgccc gcagcaagat
     5581 tgccgcggtt atcgtcggcg tgcggaatcc gcgccagagc gaagggcacc ggctccccgt
     5641 ccaccagaag gatgcggcaa tcgcccgccg tcacgatctc gggcagatag cgctgcacga
     5701 tcgcataacg cgagccgtag gccgtcaacg attcgaacac cacattgcgg ttcttgtcgc
     5761 cgtgctcgag aacgaagatc gatttgccgc ccatgccgtc gaggggtttg cagacggcct
     5821 tgccgtgttc gcgcagaaat tcgctcatat cgtgcatgtc gcgggtgatg agggtgggcg
     5881 cgcagcactc gggaaaccag gccgtgtaga ccttctcgtt catatcccgc agaccctgcg
     5941 gccggttgca caccagcgca ccctgtccct cggcgcgatc caggatatag gtcgtgtaga
     6001 tgtattccat gtcgaacggc ggatccttac gcatcaagat cacgtcgaac tcacccaact
     6061 tggcgtcccg cgtcgcgccg cgcacaaacc acttctccag gtccttgaac acttgcagcg
     6121 gcgcgagtcg accccaggca acgccatcgc gcagccaaag gtgcttctgc tcggcataaa
     6181 agagttgcca gccgcgcgcc tgtgccgcga gcaatatcgc caaagtgctg tccttggcgg
     6241 gttttatcgc ttcgatcggg tccatgacca cgaccaggcg ggcggaggaa gacatgctca
     6301 ttccttcaag gaataaaaaa ttcgcggctc gcagtctagc ctacgtcgac gcggcgggct
     6361 gcgcctccgc tcacatgaga aaactgtgaa ccaggccacc gttgtttacg tcaccatagc
     6421 tccaaatgtc gaccccatac cgtggcttgc gcgggccgga tatgttaaaa agccacaaga
     6481 ttccaggaca tacgcacacg tagctttgag ggccccagcg ggggcggatt catggagagc
     6541 acgatcacca acggcaagcc gaaactcaca ggactcaaag ttcttgtgat cgacgatagc
     6601 aagaccatcc gacggaccgc cgaaactctt ctgaccaagg agggctgcga ggtgttcacc
     6661 gccgtcgatg gattcgacgc actctccaag atcgccgatc atcaacccga catcgtgttc
     6721 gtggacatca tgatgccgcg cctcgacggt taccaaacgt gttcgctgat caagcacaac
     6781 aaagtgttca aacaaatccc ggtcatcatg ctgtcgagca aagacggatt gtttgatcgg
     6841 gcgcgtggcc gtatcgtggg ctcggagcac tatctcacga agccgttcac caaagacgaa
     6901 ttgctcggcg cgatcgaagc tcacgtcggt aaatgacgga gaccataaga atggcattaa
     6961 ttctcatcgt tgacgattca cccaccgagg tattcgtcat gcagaaggct ctcgagaagc
     7021 atggcttcaa gaccgcggcg gccgagaatg gcgaagaggg cgttcgcaaa gcgaaggcca
     7081 tgaagccgga tctcatcttc atggacatcg tgatgcccgg ggtgaatggc tatcaagcta
     7141 cccggatgct catcaacgac cctgaaacca gcctgattcc aatcatcatg gtgaccacca
     7201 agggtcaaga gaccgatcgg gtgtggggtc tgcgccaagg cgcggtggat tatttggtca
     7261 agccggtgtc ggcgcaggcg ttggtcgaga aggcacaggc cgcgctggcc gtatgacgag
     7321 catgagcggg accgagaaca gcttgcgcag cttgcgcgac cggccgttcg aattgctcaa
     7381 ggagttggag aaacgcagcc gcgcggtgag cgccagcagc gttccagaag ccagtgccgg
     7441 gcaagagtgg gtgggagtgg cgtttcgcat gggcggcgag acgtttttgg tggcgcgcga
     7501 ggaaactcgc gaggtattgg gctatcctgc ggtggtcacg cgcatccccg gcgcgaaaag
     7561 ctgggtcaag ggtttggcga acgttcgcgg gcagctgctg cccatgctgg acctccggca
     7621 atttttgggc agtggcgcca ccgcctcgag ccgcaacacc cgggttgtcg tggtcaacca
     7681 ccgggatatc ccggcggggc tcatggtcga cgaagtgttg ggatttcggc gtttcgcgga
     7741 agcggagttc aacgccgaag cgcctccgac cgtgatccgc tgcgatagct atcttgcggg
     7801 tgcctttcgc cgcggcggcg aagtatggcc ggtgttgagt ttgaagagtt tggtggagag
     7861 ccagagcttt ttacaggccg cgagctaagt tcctgtggaa caacgatgac gacgcgaaat
     7921 gatacgggtc cggggccgaa tttcttgcca gtgctggcgg cgctcatcgt actttgcttg
     7981 ggtagcgccg ccggtctgac cctgctcacg cacaatgccg ctccggtggc ggcacccgcc
     8041 gagctgtccc tcgtggtgca gcgcattccc ttcgaggcgc aaaacgcctt acgtggcgaa
     8101 tccagcgcat tcgacgcgct cgcgaagagc gcggcacggc tcaagacgct gcgcgccgcg
     8161 gcagcggaca ctgcgccggc cagcgccggc aattggaaca aaatgaccga cgatgtgagc
     8221 gcggtcagcg aggctcgagc cgcggtcgcg accattcagg gttcgagtca ggaggctcgc
     8281 gaactggcgc ccaagctgtt gtcggagctc ggcgatctgg cgagcgcggt cggtgtgcaa
     8341 aagctcgagg gcatgagccg ctatctggag cgattcgagc tcatggttca gcgcatccag
     8401 caagatttga acggccttgc gaccggcgtc agcgacgcgg cgccggccgc acagcgcctc
     8461 gccgatagtt tggagttcct gaatcaagtg atgcgcggat tatccggcga ggaatccggt
     8521 ctggcgctga cgcgggtcac cggcgccgac gccgagcaac gcttgaagac ggtggttgcc
     8581 cgcaatcagc aatatggaac cagcgtgcgc aaggcgatcg gagccgccga agcgttgtcg
     8641 aaggcgcaaa cggcggcgcg tacgctaccg gccgtggcgg gcagcctggc gacccaattg
     8701 ggggatgttc cggcgcgcac cgatgccgga gcgagttaca agccgtggat tttgtccttg
     8761 ctgggagccg ccgctttgct gctggcgggg ctcgcttgga tctacctgaa aacggtcaac
     8821 gtgcgccgca gcagcgaatt gcgcgtgcgc gagaacgagc gcaatcagga agccatcatg
     8881 cggctgctcg acgaactgtc gagtctcgcg gacggcgatc tgacggtgca agcgaccgtg
     8941 acggaggata taaccggcgc gattgccgac tcgatcaact acgcgatcga ggcgctgcgc
     9001 gagctcgtca ccaccatcaa cgacagcgcg attcagctcg atggcgccac caagcaaacc
     9061 caagcagccg catcgcacat ggcgaaggcc tcaagcgcac agtcccggca aatctcctcg
     9121 gccagcgaat ccatggtcga tatggctgcc tcgatcgagg aggtctccgg caactcggag
     9181 cgctgctcgg acgtcgcgcg gcattcggtc gatatcgctc acaagggagg cgatgccgtg
     9241 cggcggacca tcgacggcat gaacgcgatc cgcgagacca tccaggagac cagcaaacgc
     9301 atcaagcgcc tcggcgagag ttcgcaggaa atcggcaata tcgtcgaatt gatcaacgat
     9361 attgccgagc aaaccaatat tctggcgctg aatgcgtcaa tccaagcgtc gatggcgggc
     9421 gaggcggggc gaggttttgc ggtggtggcc gatgaagtgc agaggctggc ggaacgtgcg
     9481 gcgaacgcca ccaagcaaat cgaagttctg gtgcgcacca tccaaaccga caccaatgag
     9541 gcggtagttt ccatggagcg cagcaccacc gatgtggtgg gcggggcctt gctggcagag
     9601 aacgccggcg ccgccctcga ggaaatcgag caggtttcga accaaatcgc gagtttggtg
     9661 cagaacattt ccgcgagcgc gcgccagcag gcggttgctt ccggcaatat ctcgaagaac
     9721 atgcaggtgg tgcgggaaat ttcgacgcag gcggccgccg gcaccacggc gacgtccacc
     9781 tcgatcgcga aacttgcggc attgtcggct caattgcgca agtccgtggc gggcttccgg
     9841 ctgccggact acggcggcgg cacgaccacg ataggggcga actccgcgtc cgcttcctcg
     9901 ccccggctgc cgccggtgaa cgagcccgcg aacaacggcg ggtcgggcgc caagatccgc
     9961 aaggcctcag gcttcggcgc ctaggcgcaa accgattccg gcatgaccga catcagttcc
    10021 caaaccctgc atatcgtcgc gaaagaactc gcggtgacgt tgaacgacgc gcgcgcctcg
    10081 ctcgaggcgt atgccgaacg gccggatcaa caagaattgc tcgagaagtg cgccgaccag
    10141 ctgcattcgg cccacggcgt gctgcgactg gtcgaggtct acggcgcggc gctgctcgcc
    10201 gaggaaatgg agcacgtcac ccgatacctg ttgagttcgg acaacgacca gaagcgccaa
    10261 gtcgacggct tggacgcctt gatgcgcgcg atggtgcagc tgccgacgta cctcgagcgg
    10321 gtgatgtcgg gcggccgcga cctggccttg gtgctgctgc cgctgctcaa cgatctgcgt
    10381 tcggtccgcg gcagcccgtt gttgtccgag ggaacgctgc tactgctcaa tttgaaatcc
    10441 gaccagccgg cgcagccgca agcgcagcgg caaggcgaac agatcatctc ggtggcgcaa
    10501 tgggcgcggc gactacggcc gcgattccag atcggcctgt tgggatacat tcgcggcgag
    10561 cgcaccgagc aaaacctcga gatcttggca aaggtcgccg acaagctcga gcaggtcgcc
    10621 accagccagc cggtgtttca attgtggtgg gtcgtcggcg cgatcctcga ggcggttcgc
    10681 gcgaatggct tggaaggcag cgccacgctc aagcgtttgt tgggtcaagc ggaccggcag
    10741 atcaagacgt tgtacgaact cggcgaggcc cgctattgcg aagatccgcc gatcgacctg
    10801 ttgaacaacc tgttgtacta cgtcgcccgc tcgacgagcg gcggcggccg catcgccgcg
    10861 gtgcgcgctt cattcaagct caccgaactg ttgccggtcg acgattcgat cgagcaggaa
    10921 cgcgagagtc tgtcggcgcc gagcgtcaag ctcatgcgca cggttgccgc cgccatcaag
    10981 gaagacctgt ccaaggtcaa ggacgttctc gatatcttcg tgcgtcgcgg cggcggccgc
    11041 agcgaggaac tcataccgca gctcgagttg ctgaaaaaaa tcagcgacac gctcggggtt
    11101 ctcggactcg gcgaactgcg gcagcgggtg caggaagaga tctccgagct ttccgatatc
    11161 gtcaccgacg gcaagatacc gccggatgag tcgttgatca aagtggccgg agtgctgctg
    11221 tcggtcgaag acagcctcga tgatcaattg gtgcgcctga ttttgccggc gacctccgag
    11281 cggcccctca ccgaggtttc gggcgaccag gacctcgagt acaaactggt gagcgaggcg
    11341 gtattgcgcg agtgcatcgt caatttggcc cgcatcaaag aggctgtctc gcttgcggtg
    11401 cataagcccg gcgatgcggc gccgcagggc ctcgacaacg taccgcaact gctgcgcggc
    11461 ataactgccg gtctcttgat gctgggcaag ggccgggcgg tcgaattgat ggatgcgatc
    11521 ggcacagagg tacgcaagct catcgagccg ggtgccccgc cgccggaacc cattcgcttg
    11581 gagcgggtgg cggacgccat cgtcagcatc gagtactaca tggagacgct gcagagcgga
    11641 cgtaccgatc catggtacat gctcgacaat gcggagacct gcctcaagac gctcgaggag
    11701 caggcgcctt cgcgggtgcc caatatcagc atctcgagca gcgaagccgc gaaaacagtc
    11761 aagatcgatc ctatccagac ggtgcagttg gaacgcgcca agcgtacgca tgcagcaacc
    11821 catccgctga tcgtggttcc cgagcccgag cccgtcgatc cgcaattcct ggagctgttc
    11881 atcgaagaag ccaaggaaga gatcgcctcg attcaaaaca gcttcccgct ctgggaccaa
    11941 aatcccatgg acctcgagtc gttggcgttg atgcggcgca gtttccatac gttgaagggc
    12001 agtggccgca tggtgggtgc ccggtcgatc gcagagttcg gctggtcgat cgagaatctg
    12061 ctgaatcgga tcaccgacaa gacgctgtcg cgtacgcctg gaatgatggc gctgttgcgc
    12121 agttcaatcg cggcgctgcc gcaattggtc gagcacctgg aaacgggccg gcaaaacacg
    12181 gtgccgatcg aggcgctgat ggcgcgcgcc ttcgcgtatg ccgacggccg agaagccgat
    12241 caggggacga tcaccctcac gccggcggat cgtgaaatgg cgg
//
LOCUS       DASWZT010000125         4081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_39764, whole genome shotgun sequence.
ACCESSION   DASWZT010000125 DASWZT010000000
VERSION     DASWZT010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39764"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VGE92_03455"
     CDS             <1..775
                     /locus_tag="VGE92_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312903.1"
                     /translation="RAFRNSCSEAPHILNAALSGHVEVIRCAIHGLQFELDGSRLGER
                     GRADLTPLDLRMLAGLILVRSERVHAPRAADPWSEFSPPAGFQPIGPLGDATLAADWK
                     LVIEQWLASAQRTTSRPDAQAWSVRRYRNLLAPAADSAWQRRFIAPNHLIELRPDGFS
                     LLQVVPAGPGQSRVRRFDYSVCEPKRRALAARYLASRLNPYTRHSAIAIAESTQQGIA
                     TFGHEVAAGTPSAPAAVAFRRQLAAFVPMLALARPPHEI"
     gene            824..2323
                     /locus_tag="VGE92_03460"
     CDS             824..2323
                     /locus_tag="VGE92_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY1312904.1"
                     /translation="MNLTSLTTQDWHARAKAVRYETRHFIDGQYTDSVARGRFTVVNP
                     ATGEPLCAVSAGTAADIDLAVAAAKRSFAARVWSRKAPRDRMAIMATYARLLEENNEK
                     FSLLDTLCMGKPITDMVTIDVPAAARNFAYFGELIDKIDGTVTATAQDAFHYILREPL
                     GVVGCIVPWNYPLLMAAWKVAPALAAGNSVVLKPAEQSPLSGALMAQLFIEAGGPPGV
                     FNVVNGVGEVAGAALALHKDVAKIAFTGSTEIGKQMLIYAGQSNMKRVSLECGGKTPQ
                     IFLADLPDLDTAVTYAINGIYGNMGEVCNAGSRLLLDRPIAKEFLARFIERGKDAYRA
                     GDPLDPTTNLGPLVTASHRTRVMSYITRGKSEGARLEFGGTSPDLPGAFVNPTLFTGV
                     NNRMTIAQEEIFGPVAAAIEVDGLSEALAVANDSIYGLAASVWTRDLSVAHKTVRDLE
                     AGVIWVNCFDHGDMTQPFGGYKQSGQGRDKCVESLLSYTQTKSAWINLG"
     gene            complement(2354..3520)
                     /locus_tag="VGE92_03465"
     CDS             complement(2354..3520)
                     /locus_tag="VGE92_03465"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY1312905.1"
                     /translation="MTNLSVATRTDATPDTLPHALPAWVYSNSELNRLELERILLPSW
                     QIVCHVNSIPNPGDYETFDLGPESVMVLRDRDGSIRGFHNVCRHRGARLLDGSGNCPA
                     TITCPYHGWTYRHDGGLIGMPVRDSFPGLDRAQHGLRTVRTDIAFGFVFVCLSGDPPP
                     VAKLWGKLADEFLPYRFEEMVPLGPISQETWNVDWKIAMDNYLESYHVPIGHPGLYRM
                     FTPDYEDQASVPGIARGISWLRKQDSPRWAEGLYQRMIGNVATHLPEEQRRCWRFYSA
                     LPNLGIDVFPDQMDFFQVLPAGPGKCTIRGAVFGLPDERREMRAVRYLSSRINTQVNN
                     EDKWLCGRVQRGLASGSYKPGPLSQLERWMFEFHNLLRERIPEFALPSAPKQFA"
     gene            3789..>4081
                     /locus_tag="VGE92_03470"
     CDS             3789..>4081
                     /locus_tag="VGE92_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY1312906.1"
                     /translation="MARNLTKNNAHFQRAVKRLPLGVASNFRFWGEDRTIYVKSGAGA
                     RITDLDDNNYVDYRLGYGPAILGYAHPEVDQAARDGMAVGGVFALSTERELSVA"
BASE COUNT          767 a         1266 c         1317 g          731 t
ORIGIN      
        1 gcgcgcgttt cgcaatagct gcagcgaggc cccgcacatc ctgaacgccg ccctcagcgg
       61 acacgtcgag gtcattcgtt gcgcgattca cggtctgcaa ttcgagctcg acggcagccg
      121 cttgggcgag cgcggcaggg cggacctgac gcctttggac ctgcgtatgc tcgcggggct
      181 gattttggtc cgctccgagc gagtccacgc gccccgcgcg gccgatcctt ggagcgaatt
      241 ttcgccgccg gccggattcc agccgatcgg gcctctcggc gatgcgacgc ttgcggcgga
      301 ttggaagctc gtgatcgaac agtggctggc gtcggcacag cgcacgacat cgcgtccgga
      361 tgcgcaggcg tggagcgttc gccgctaccg taatctgctg gcgcctgccg cggattccgc
      421 gtggcagcgg cgattcatag cgccgaatca cctcatcgaa ctgcggccgg acggctttag
      481 cctattgcag gtggtgccgg cagggcccgg ccaaagccgg gtgagacgct tcgactacag
      541 cgtgtgcgag cccaagcgcc gggcgctcgc tgcccgctac ctggcatcgc gtctcaatcc
      601 gtacacgcgg cacagtgcca ttgcaatcgc cgaatcgacg cagcaaggca ttgcgacctt
      661 tggccatgag gtggcggcgg gaactccgtc cgcgccggcg gcggtcgcgt tccgccggca
      721 actcgcggcc ttcgtcccga tgctggcgct ggctcggccg ccgcacgaga tttgaataaa
      781 ttcactatac tgcgcgcgac cgcgcaacca acgggagtac cccatgaatc tgacgtccct
      841 gactacccaa gactggcacg cccgtgccaa ggccgtgcgt tacgagacgc ggcatttcat
      901 cgacggccaa tacacggatt cggtcgcccg cgggcgcttc accgtggtca atccggcgac
      961 cggcgaaccc ttgtgcgcgg tgagcgccgg caccgcggcc gacatcgacc tggccgtggc
     1021 agccgccaag cgcagcttcg ccgcgcgggt ctggagccgc aaagcgccgc gcgatcgcat
     1081 ggcgatcatg gcaacctacg ctcggctcct ggaagagaac aacgagaaat tctcgctcct
     1141 cgatacgctg tgcatgggca agccgatcac cgatatggtc accatcgatg tgccggcggc
     1201 ggcgcgcaat tttgcctact tcggcgaact gatcgacaag atcgacggca ccgtcacagc
     1261 gaccgcccag gatgcgttcc actacatttt gcgcgaacca ttgggagtgg ttggttgcat
     1321 cgtgccgtgg aattacccgt tgctgatggc ggcctggaaa gtcgcgccgg cgctcgcggc
     1381 cggaaattcg gtggtgttga agccggccga acaatcgccg ctgtcgggcg ccttgatggc
     1441 gcagctgttc attgaggcgg gcggcccgcc gggcgtattc aacgtcgtga acggcgtggg
     1501 cgaggtcgcc ggcgccgccc tggcgctgca caaggacgtc gccaaaatcg ccttcacggg
     1561 ctcgacggaa atcggcaagc aaatgttgat ctacgccggc cagtcgaata tgaagcgcgt
     1621 cagcttggag tgcggcggta aaaccccgca gatatttctc gccgatctgc ccgatctgga
     1681 taccgcggtg acttacgcga tcaacgggat ctacggcaac atgggcgaag tgtgcaacgc
     1741 gggatcgcgc ttgctgctgg atcggcccat tgccaaggaa tttctggcgc gcttcatcga
     1801 gcgcggcaag gacgcgtatc gggcgggcga tccgcttgat cccaccacca atttggggcc
     1861 gctggtgacc gcctcgcatc gaacgcgggt gatgagctac atcacacgcg gcaagtcgga
     1921 gggcgcacgg ctcgaattcg gcggcacctc gccggatctt ccgggagcct tcgtcaatcc
     1981 gacgctgttc accggcgtga acaatcgcat gaccatcgcg caagaggaga ttttcggacc
     2041 ggtggcagcg gcgatcgaag tcgacggttt gagcgaagcg ctcgcggtgg ccaacgactc
     2101 gatctatgga ttggcggcct ccgtctggac gcgcgattta agcgtcgccc acaaaacggt
     2161 tcgcgacctg gaggcgggcg tcatctgggt gaattgcttc gaccacggcg acatgacgca
     2221 acccttcggc ggctacaaac agtccgggca aggccgggat aagtgcgtgg aaagcctgct
     2281 ctcgtacacg cagaccaagt ccgcctggat caacctgggc tgagcgcggc gggcagcggg
     2341 gtgcgggccg cgctcaggcg aattgtttgg gcgcgctcgg cagcgcgaat tccgggatcc
     2401 gctcgcgcag cagattgtgg aattcgaaca tccagcgctc gagctgcgac aagggacccg
     2461 gcttgtagct gccggaggcg agtccgcgct gcacccgacc gcacagccac ttatcctcgt
     2521 tgttcacctg cgtgttgatg cgcgagctca agtagcgcac cgcgcgcatt tcgcgccgct
     2581 catcgggcag gccgaaaacg gcgccgcgaa tggtgcattt gcctggaccc gccggcagca
     2641 cttggaaaaa gtccatctgg tcgggaaaca catcgatccc cagattcggc agcgcgctgt
     2701 agaagcgcca gcaacggcgt tgctcttcgg gcaaatgggt ggcgacgttg ccgatcatgc
     2761 gctgatacag tccctccgcc cagcgcggcg agtcctgttt gcgcaaccag ctgatgccgc
     2821 gcgcgatccc gggcaccgag gcctgatctt cgtagtccgg tgtgaacatg cgatacaggc
     2881 cgggatgtcc gatgggcaca tgatacgact cgagatagtt gtccatcgca atcttccagt
     2941 cgacattcca ggtctcttgg gagatgggtc cgagcggtac catttcttcg aaccgatagg
     3001 gcaggaattc gtcggccaat ttcccccaga gcttcgccac cggcggcgga tcgccgctca
     3061 ggcagacgaa cacgaaaccg aacgcaatat cggtgcgaac cgtgcgcagg ccatgctggg
     3121 cgcggtcgag ccccgggaag ctgtcgcgga ccggcatgcc gatcaggccg ccgtcgtgac
     3181 gataggtcca accgtgatag gggcaggtga tggtggcggg gcagtttccc gagccatcca
     3241 acaggcgcgc gccgcggtgc cggcaaacat tgtgaaagcc gcggatcgag ccatcgcggt
     3301 cgcgcagcac catgacactt tccggaccca ggtcgaacgt ctcgtagtca cccgggttcg
     3361 ggatggaatt gacgtggcac acgatctgcc agctgggcaa cagaatgcgt tcgagctcga
     3421 gccggttgag ctcgctgttg ctgtaaaccc acgccggaag ggcatggggc agggtgtctg
     3481 gcgtggcgtc ggtgcgcgtc gcgacggata ggttggtcat ggcttccccg acagcgatta
     3541 tatgatcatt taatgtactc tgaaggctgc aaggttggca agtcgaagca gcgcagcagg
     3601 gcaattcagc acgaaagtgc cgattcggag cgtcgtacgc gcaattgcgg gggcgtctcc
     3661 cacgcgcttg cggcgtgcat cccaagcatt tattatatga tcatataata aatctggacg
     3721 agacccgcgg cgagtgctac ggtctcacaa aatcctaccg attgcccatt caaaataagg
     3781 ggtacgtcat ggccagaaat ctgaccaaga acaacgcgca ttttcagcgc gccgtcaaac
     3841 gcctgccgct cggtgtggct tccaacttcc gcttctgggg ggaagatcgc accatctacg
     3901 tgaaaagcgg cgctggggcg cgcatcacgg atctggacga caacaactac gtcgattatc
     3961 gcttgggcta cggccccgcg atactcggct acgcgcaccc cgaggttgat caagccgccc
     4021 gcgatggcat ggctgtcggc ggagtcttcg ccttgtcgac cgagcgtgaa ctcagcgtgg
     4081 c
//
LOCUS       DASWZT010000126         5083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_30024, whole genome shotgun sequence.
ACCESSION   DASWZT010000126 DASWZT010000000
VERSION     DASWZT010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30024"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /locus_tag="VGE92_03475"
     CDS             complement(<1..1260)
                     /locus_tag="VGE92_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019691486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1312907.1"
                     /translation="MASSYTASDIEVLSGLDPVRRRPGMYTDTTRPNHLAHEVIDNSV
                     DEALAGHCDNINVTVFKDGSLQVEDDGRGMPVDIHPKEKISGVELILTRLHAGGKFND
                     KTYQHSGGLHGVGVSVVNALSKHLECWVRRGGKEYNVSFAGGRLKSKLEVVGDVAKSK
                     TGTTLRFWPDPQYFDTDKFAIPKLKQVLRAKAVLCPKLRVTLSNEFTGERDEWLYSGG
                     LHQYLAEELGKGEWLPAETFYGRRDIDGGSLDWAFAWVLESEHSISESYVNLIPTVEG
                     GTHVNGLRAGAADAVREFCEFRNLVPRGLKLTPEDVWDGVSFVLSMKMREPQFAGQTK
                     EKLSSRESAGIVQGVIKDAFALWLNQHPDQGEKIAMLAIANAQQRFKDSQKIARKRIV
                     AGPALPGKLADCVSQDPARSELFLVEGD"
     gene            1355..1621
                     /locus_tag="VGE92_03480"
     CDS             1355..1621
                     /locus_tag="VGE92_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312908.1"
                     /translation="MGIANAQNLVRNLPVLPRPYGIRVRLRSGDPFAKLLGTDWQKTH
                     WFFTAAERDRVLEDMSRKHEYSRIGDQPALVFEKVEKLSESRNF"
     gene            complement(1624..3912)
                     /locus_tag="VGE92_03485"
     CDS             complement(1624..3912)
                     /locus_tag="VGE92_03485"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY1312909.1"
                     /translation="MLGKRSTRLRCTVAIFAGCVCCASNAARAAGAAAHESTRPRICL
                     VLSGGGARGMAHIGVLKILEDLKIPIDCIAGTSMGAIVGGLYASGMTAAQIDVTMRSL
                     DWQEAFRDAPPRRDLAFRRKQDDRNFLVELPLGLKHGKVLLPKGFIQGQKLQETLRQL
                     TLPFSNSTDFDLLPTPFRAVATDLETGNAVLMDKGDLSIAMRASMSAPGVFAPVELNG
                     RLLVDGGLAENLPINVARAMHADILIVSDVSFPLQPRADLDSALSISNQMLAILVRKD
                     SDRQRASLGPQDILIEPELGAATATDFASPGAVITHGEDAAREAIPRLSAYAVGDAAY
                     GEYLARRAARQPGLPPIQFVRVDEQSKRYEKTILAEMQPLVGKPLDLDEVGKRITELY
                     GLGNFETLDYTLVDQSLSGGPPGTAAANGDEESGLEVRARRKSWGPNYLRFGLNLQDD
                     FQGNSRYNAAARFVLSEINELGAEFLTDLQIGSDPKVVSEFYQPLDATRTWFVAPSAR
                     IEARDLPIYSNNLEVADFRDREAEADLDFGRNLGNWGEIRAGFHRTNGETHARLGDPD
                     LVEQQYNLGEYFFKFSYDRLDNVHFPREGQTFSLQWDANRTNLGADIASDRVTADWLM
                     ARSRGRNTILLWTSAGTTLDGNFKPIDLPEFYSLGGFFNLSGLAPGSLIGPNYAIARA
                     IYFRKIGRGGEGFFEFPAYIGMSLELGNTWEHRGDIGYGSARKDASLFLAFDTFLGPV
                     YIGSGYDQSGTAAFYLFLGRTF"
     gene            complement(3924..>5083)
                     /locus_tag="VGE92_03490"
     CDS             complement(3924..>5083)
                     /locus_tag="VGE92_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312910.1"
                     /translation="AYTTFVPSVNDVYSPPAAAGTFYPLYVVGGSSLEDFYTEGLTGY
                     VTARKGNTLTVSGSTLIENSSEITTYQDAAAQLIVGSGTIVTADGTTLTGLNSDSIGV
                     GQYISARGLYTLPASNVVTVDATGTTSTNTGSVRILPTEIWGSLVSSASGSLSMNLQT
                     VNDWPVSSYNFAGNGVSTAQDPLPAAFSVDTGALPLPSGLVAGDPVWISGMVSPFGTA
                     PPDFKASAVNSEASVQVAGGAESVPGTQTCGVGSQVCDPASLRVVWSAAGTNTPFVGL
                     ANTGFTIDLSNAQYVSGVIRIGPESIDLKSLPASPQIVPTSLPLTATFAPRFAVGNPS
                     TGIDVFSGFAAFVTQVHTSVNGTTPALQFEARGLYNRTTNTFTATTVNIVL"
BASE COUNT          955 a         1590 c         1556 g          982 t
ORIGIN      
        1 atcgccctcg accaggaaca gctcgctgcg cgccgggtcc tgggaaacgc agtcggcgag
       61 tttgcccggc agcgcggggc cggcgacgat gcgcttgcgc gcgattttct gactgtcctt
      121 gaagcgctgc tgggcattgg cgatcgccag catggcgatt ttctcgccct gatcgggatg
      181 ctgattcagc cacagtgcaa aggcgtcctt gatgacgcct tggacgattc cagccgactc
      241 gcgcgatgaa agcttctcct tggtctggcc ggcgaactgc ggttcgcgca tcttcatcga
      301 cagcacgaag ctcacgccat cccagacatc ctcgggcgtg agtttaagac cgcgcggcac
      361 gagatttcga aactcgcaga attcgcgcac cgcatcggct gctccggccc gcaggccgtt
      421 gacgtgcgtg ccgccttcga ccgtcggaat caaattgacg tagctttcgc tgatgctgtg
      481 ctcggactcg agcacccagg cgaacgccca atcgagggaa ccgccgtcga tgtcgcgcct
      541 gccgtagaaa gtctcagccg gcaaccactc gcccttgccc aactcctcgg ccagatactg
      601 atgcagtcct ccgctataca gccattcgtc gcgctcgccg gtgaattcgt tgctgagcgt
      661 gacgcgcagc ttcgggcaca gcaccgcttt cgcgcgcaga acttgcttga gtttcgggat
      721 cgcgaacttg tcggtatcga aatactgcgg gtcgggccag aagcgcagcg tggtgccggt
      781 cttcgacttt gccacatcgc cgaccacctc gagcttggac ttgaggcggc cgcccgcgaa
      841 gcttacgttg tattccttgc cgccgcgccg cacccagcac tccaggtgct tcgacagcgc
      901 gttgaccacc gacacgccga cgccgtgcag accgccggaa tgctgatagg tcttgtcgtt
      961 gaatttaccg ccggcgtgca gccgcgtgag gatcaactcc acgccgctga tcttttcttt
     1021 ggggtgaatg tcgaccggca tgccgcgccc gtcatcctcg acctgcaggg aaccgtcctt
     1081 gaacacggtg acattgatgt tgtcgcaatg gccggccagc gcctcgtcca cgctgttgtc
     1141 gatgacttcg tgcgcgagat ggttcggccg cgtggtatcg gtatacatgc ccgggcgccg
     1201 gcggaccggg tccaggccgc tcaatacctc gatgtcggag gccgtgtaag acgatgccat
     1261 cggttacctc gtgtgaaggc ggcggagtct agcacgcgac tcgcgcccta atcagctggc
     1321 aggcgagcca cgattcggct atcttccggg catcatggga atcgcaaatg ctcagaatct
     1381 ggtgcgcaac ttaccggtgt tgccgcgccc ctatggcatt cgcgtgcgcc tgcggtccgg
     1441 cgatccgttt gcaaaattac tcggcaccga ctggcagaag actcattggt tcttcaccgc
     1501 ggcggaacgc gatcgggtcc tcgaggacat gtcacgcaag cacgagtact cgcgaatcgg
     1561 cgaccagccc gcgttggtgt tcgaaaaagt ggagaaactc tccgaaagcc gcaatttctg
     1621 attttagaac gtgcggccca ggaacaagta aaacgccgcc gtcccacttt gatcgtagcc
     1681 gctgccgata tacacgggtc cgagaaaggt gtcgaacgcc agaaatagcg atgcgtcctt
     1741 gcgcgccgat ccatagccga tatcgccgcg atgttcccag gtattgccga gttccaagga
     1801 catgccgata tacgcgggaa actcgaaaaa tccctcgccg ccgcggccga tttttcgaaa
     1861 atagatcgct cgcgcaattg catagttggg gccgatcaac gagcccggcg cgagccccga
     1921 cagattgaaa aagccgccca aggaatagaa ttcgggcagg tcgatcggct tgaaattgcc
     1981 gtcaagggtg gtgcctgccg aggtccacaa caaaatcgtg ttgcgtcccc tggagcgcgc
     2041 catcaaccag tcggccgtga cgcgatcgga cgcgatgtcc gcgcccaagt tggtgcgatt
     2101 ggcgtcccat tgcaggctga aggtctggcc ttcgcgcggg aaatgcacat tgtccaggcg
     2161 gtcgtagctg aacttgaaga aatactcgcc caaattgtac tgctgctcca ccagatccgg
     2221 atcgccgagg cgcgcgtgcg tctcgccgtt ggtgcgatga aacccggcgc ggatttcgcc
     2281 ccagttgccg aggttgcggc cgaaatcaag gtcggcctcg gcctcgcggt cgcgaaaatc
     2341 cgccacttca agattattgc tgtagatcgg tagatcgcgc gcttcgatcc gcgcgctcgg
     2401 cgccacgaac caggtgcgtg tcgcgtcgag cggctgatag aactcgctca ccaccttggg
     2461 gtcgctgcca atttgcaggt cggtgaggaa ttccgcgccg agctcgttga tttcagacag
     2521 cacgaagcgc gccgccgcgt tgtagcggct attgccttgg aagtcgtcct gtaaattgag
     2581 accgaaacga agatagttgg ggccccagga tttgcgccgc gcccgcacct cgagcccgga
     2641 ctcctcatcg ccattcgcgg cggctgtgcc cggcgggccc ccacttaacg attggtcgac
     2701 cagggtgtag tccagagtct cgaaattccc gaggccgtac aactccgtga tgcgtttgcc
     2761 gacctcatcg agatccaagg gtttgccgac caacggctgc atttccgcca ggatggtctt
     2821 ttcatagcgt ttcgattgct catcgacgcg cacgaactgg atgggcggca ggcccggttg
     2881 gcgcgctgcg cgccgcgcca ggtattcgcc gtacgcggca tcgccgacag cgtacgcgga
     2941 caacctgggg atggcttccc gcgccgcgtc ttcgccgtgt gtgatgaccg ctcccggaga
     3001 cgcaaaatcg gttgcggtcg cggcgcccaa ttcaggttcg attaaaatgt cctgcgggcc
     3061 caagcttgcc cgctgccggt ccgagtcctt gcgcaccagg atcgccagca tctggttgga
     3121 gatcgacagc gcagaatcca agtcggcgcg aggctgcagc ggaaaactca catcggacac
     3181 gatcagaatg tccgcgtgca tcgcgcgtgc cacgttgatg ggtaggttct ccgccagtcc
     3241 gccgtcgacc aacaggcggc cgttcagttc caccggcgcg aacacaccgg gcgcggacat
     3301 gctggcgcgc atggcgatcg aaagatcgcc tttgtccatc agcacggcgt tgccggtctc
     3361 caggtcggtg gcgaccgcgc gaaatggggt gggcagcagg tcgaaatcgg tgctgttgct
     3421 gaacggcagc gtcaattgcc gcagcgtttc ttgcagcttc tggccttgta tgaatccctt
     3481 cggcagcaag actttgccgt gtttgaggcc gagcggcagt tccaccagga agttgcggtc
     3541 atcttgcttg cgcctgaatg ccagatcgcg gcgcggcggc gcgtcgcgaa acgcttcctg
     3601 ccaatcgagc gagcgcatgg tgacgtcgat ttgcgccgcc gtcatgccgc tcgcgtacaa
     3661 cccgccgacg atggcaccca tgctggtgcc ggcgatgcag tcgatcggga tcttcagatc
     3721 ttcgagtatc ttgagtacgc cgatgtgcgc catgccgcgc gcgccaccac cggacaatac
     3781 caggcaaatt cgcgggcgag ttgactcgtg cgccgcggcg cccgccgctc gcgcagcgtt
     3841 gctcgcgcag caaacgcagc cggcgaaaat cgccaccgtg cagcgcagtc gagttgagcg
     3901 tttccccagc atggggcgct agtttagagc acgatattga cggtggtcgc ggtaaaggtg
     3961 ttggtggttc ggttgtacag gcctcgggct tcgaactgca gtgccggagt cgtcccattc
     4021 accgaggtgt gcacctgcgt gacaaacgca gcgaagcccg agaacacatc gatgcccgtg
     4081 cttggattgc cgaccgcgaa tcgaggcgcg aaagtcgcgg tcaaaggcaa actcgtcggc
     4141 acgatttgcg gactggccgg cagggacttc aggtcgatgc tttcgggtcc aattcgaatt
     4201 acgcccgaaa cgtattgcgc attgctgaga tcgatggtga accccgtgtt cgccaagccg
     4261 acgaacggcg tgttcgtgcc cgccgcgctc cagactaccc gcaagctggc cggatcgcag
     4321 acctggctgc cgacaccgca ggtctgggtt ccgggcaccg actcggcacc ccccgccacc
     4381 tgaacggagg cctccgaatt gacagcggaa gccttgaaat ccgggggcgc cgttccgaac
     4441 gggctcacca ttccgctgat ccacaccgga tcgcccgcga ccaagccgga tggcaacggc
     4501 agcgcgccgg tgtcgaccga aaaggcggcc ggcagcggat cctgtgcggt actcacaccg
     4561 ttgcccgcaa agttgtagga actcacaggc caatcgttca ccgtttgcaa attcatgctc
     4621 aggctgcccg acgccgatga caccagcgac ccccaaattt cggtgggcaa tatgcgcacc
     4681 gaaccggtgt tggtgctggt agttcccgtg gcatcgacgg tgacgacatt tgaagccggc
     4741 aatgtgtaga gcccgcgcgc gctgatgtac tggccgactc cgatcgaatc cgagttcagg
     4801 cccgtgagtg tagttccgtc ggcggtcacg atggtgcccg agccgacaat cagctgcgcg
     4861 gccgcatcct ggtaggtggt tatttccgaa ctattctcga tcaatgtcga cccgctcacc
     4921 gtcagggtat tccccttgcg cgccgtcaca tatccggtca agccttccgt gtagaaatcc
     4981 tcgagcgaac tgccgccgac gacgtacagc gggtaaaagg tcccggccgc cgcgggcggc
     5041 gaataaacgt cgttgacgct cggcacaaac gtagtatacg cgg
//
LOCUS       DASWZT010000127         6568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_68672, whole genome shotgun sequence.
ACCESSION   DASWZT010000127 DASWZT010000000
VERSION     DASWZT010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6568
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68672"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1628)
                     /locus_tag="VGE92_03495"
     CDS             complement(72..1628)
                     /locus_tag="VGE92_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312911.1"
                     /translation="MFSPIAPQTAPAGSFDLLDRAAIGYVCLPLVIFLAGWFEWWAAL
                     PLIACTAYALRSLIAKWPRGPSRLPITPLQLAVAVIVGGAWTVLGGTDHLVFANADWH
                     IRDAVLHDLVVSPWPVGYGLVDGQESLLRAPVAYYLPAAIVGKAAGLAAAHVSMLVWT
                     AIGAILFLLQVLSLTRSRRSVAITVAAVVVFFSGFDIIGSLLNDGPRFRSDWNITTHL
                     EWWAGSYQYSSMTTQLFWVPNHALGGWLLIGLLYRDDRSTRLDSILPMLFVASALWSP
                     LTAIGLIPFVAWKWVSSMRAERSWRLLHPQVWLPALVVGVALVTFLALDPGRIPKGFA
                     LAKDSPGGAVMSLLRQAQFFLLEAGILGIAILAIRRRSEVVVALVVLAVLPFAYLGGG
                     NDLVMRASIPSLAVLTIAAGLALVADATEPHATRKKIILAGLLAVGAVTPIEELARAV
                     ILPVWPINTRATLIGASCGAYAPHYVARLSSAAIAPLLRPPNQLSLGPLDRAACFNPA
                     IELMWRGHPL"
     gene            complement(1745..2428)
                     /locus_tag="VGE92_03500"
     CDS             complement(1745..2428)
                     /locus_tag="VGE92_03500"
                     /inference="COORDINATES: protein motif:HMM:NF026165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY1312912.1"
                     /translation="MSRIGSRPTVHVVAAAVIDAADRVLIAQRPRGVHLAGGWEFPGG
                     KLEVGEERRAGLARELNEELGIQIGSPRPLMRIRHAYPSREILLDMWVVRSYSGEPQG
                     LDGQALRWCTQNELAGVELLPADKPIVSALRLPERLTQAETPDYRVAEPGIAAGDAGM
                     LGIYCRDVAEGAAAASVGADFLVMRAALSAEELRGLCRCVPLPVFARGIDLAEAWALG
                     ASGVNSIAE"
     gene            complement(2425..5205)
                     /gene="secA"
                     /locus_tag="VGE92_03505"
     CDS             complement(2425..5205)
                     /gene="secA"
                     /locus_tag="VGE92_03505"
                     /inference="COORDINATES: protein motif:HMM:NF009538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY1312913.1"
                     /translation="MWKLLTQVFGSRNQRLVKELSRTVAQVNALEAGISALPDAQFPE
                     KTRELKARFAAGTSLNDLVPEAFALVREASRRKLGLRHFDVQLIGGLALHSGKIAEMR
                     TGEGKTLMSTLPAYLNALTGDGVHVVTVNEYLAQRDSEWMGPVFRFLGLSVGVIKNAQ
                     SPDEKRAAYACDITYGTNNEFGFDYLRDNLAFRLEDRVQRKLSFAIVDEVDSILIDEA
                     RTPLIISGPAEESTELYLKINALIPRLTRQKAEDGPGDYAVDEKTKQATLTEDGHQKV
                     EELMAEIGLLREGESLYDATNIRLMHHLNAALRAHALYKRDVEYIVRQGEVIIVDEFT
                     GRTMQGRRWSDGLHQAVEAKEGVKVREENQTVASITFQNYFRLYAKLSGMTGTADTEA
                     FEFQQIYGLEVVVIPTHKPMIRADKSDLVFLSEQDKFQAILEDIQDCVKRGQPVLVGT
                     TSIETSERLAKLLQGAKIPHEVLNAKQHEREAHIVAEAGRPGAITIATNMAGRGTDIV
                     LGGSPQAQIDAIENPNAAELKQIHDDWRKRHEQVVAAGGLHIVGTERHESRRIDNQLR
                     GRSGRQGDPGSSRFYLSLEDNLMRIFGDPTRMKALMTRVGMKAGEAIESGMLTRQIER
                     AQRRVEQHNFDARKQLLEYDDVANDQRRVIYQQRTDLMSAEDVAEAVKGIREDVVAQL
                     VDQYVPGGAPEEQWDIRGLTQSIERDFALTLPIDEWLKAEEAGDGSGLRSKIEAALEA
                     AYDQKQNSVGLQVMRYIEKEVMLRTLDQHWREHLAAMDYMRQGIFLRSYAQKNPKQEY
                     KREAFELFSAMLDRIKFDTLTTVSKIQVRSRAEIEREELERQQRLARALQAQHAEAIS
                     PIQATPAPGEFGGGEVVAAPGNVMEMDPRAAAAAAPFVRPLPKVGRNEPCPCGSGRKY
                     KHCHGALQQASG"
     gene            complement(5313..6239)
                     /locus_tag="VGE92_03510"
     CDS             complement(5313..6239)
                     /locus_tag="VGE92_03510"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY1312914.1"
                     /translation="MNVIFVGRNSGRMRQFDLRHPVIVAAAVLVVLAVVGGAFSVGMG
                     LGARHSSGNSVNQLGNWSAELMRQQAQLENVKRVLQEKVNALAMRVGQMNANVIRVNA
                     LGKRLTRMANLDDGEFDFGNPPALGGSESGADGQPAQIPNLSAMVDDLQTQLASREQQ
                     LGVLENLILTRELNKQVYPEGRPVQDGWISSYFGRRADPFTGYTAVHKGLDFAGPEGT
                     KVTTVAAGLVTFAGERSGFGQMVEINHGNGLATRYCHNEKVLVKQGDMVRKGQEVALM
                     GSTGRSTGPHLHFEVLKNGTQVDPLRFIGEDR"
BASE COUNT         1266 a         2095 c         1963 g         1244 t
ORIGIN      
        1 ctggccgcct cgcccggcga taagatcgcc ggctgcccat ggcgacgctc gttcggccgg
       61 tgttgcaagg atcatagtgg atgcccgcgc cacatcagct cgatcgcggg attgaagcac
      121 gccgctcgat ccagcggtcc aagcgaaagt tgattcggcg ggcgcaacaa cggggcgatc
      181 gctgcgctgc tcaggcgcgc cacatagtgc ggcgcgtagg cgccgcaact cgcgccaatg
      241 agagtggcgc gggtgttgat cggccaaacc ggcaggatca cggccctcgc cagctcctcg
      301 atcggagtta cggcaccgac cgccagcaga ccggcgagga taattttctt gcgtgtggcg
      361 tgtggctcgg tcgcgtccgc caccagagcg agaccggcgg cgatcgtcaa caccgcgaga
      421 ctgggaatcg atgcacgcat caccagatcg tttccgcccc ccagataggc gaacggcagc
      481 acggccagca caaccaacgc cacgacgact tcgctgcggc ggcgaattgc aagaatcgcg
      541 attccgagga tccccgcttc gaggaggaaa aactgagcct ggcgcagcag actcatgacc
      601 gccccgccgg gcgagtcctt ggcgagcgcg aatcccttgg gaatacgccc cgggtccaac
      661 gcgagaaaag tgacgagcgc gacgccaaca acgagtgccg gcaaccacac ctgcggatgg
      721 agcagtcgcc aggagcgctc ggcgcgcatg ctgctgaccc acttccaggc gacgaaaggg
      781 atcaatccga tggccgtcag gggcgaccat aacgcggagg cgacgaagag catcggcagg
      841 atagaatcca gccgtgtcga tcggtcgtcc cggtacagta atccgatcaa cagccagccg
      901 cccagtgcat gattgggtac ccagaacaac tgggtggtca tcgatgaata ttggtaactg
      961 ccggcccacc actcgaggtg ggtagttatg ttccagtcac tgcggaagcg cggaccatcg
     1021 ttcagcaggc tgccgatgat gtcgaatccg ctgaagaaca cgaccaccgc cgccaccgtg
     1081 atggcaacgc tccgccgtga gcgcgtcaac gacagaactt gcaacaaaaa caggatggcg
     1141 ccgatcgccg tccacaccag catggacaca tgggccgcag ccaagcccgc tgctttgccg
     1201 acgatcgccg ccggcagata gtacgcgacg ggtgcgcgca gcaagctttc ctgaccatcg
     1261 accaaaccgt agccgaccgg ccacggagaa acgaccaagt catgcagcac cgcatcgcgg
     1321 atgtgccaat cggcgttcgc gaacaccaga tgatcggtgc cgccgaggac cgtccaggcg
     1381 cccccgacga tgacggcaac cgcgagctgc agcggcgtga tgggtaatcg agaagggccg
     1441 cgcggccact tggcgatcag cgatctcagt gcataagctg tacacgcaat caagggcaat
     1501 gccgcccacc attcgaacca tcccgccagg aagatgacga gaggcaggca cacatatccg
     1561 atggccgccc ggtcgaggag atcgaaggag ccggcgggcg cggtttgcgg tgcaatgggg
     1621 ctgaacatcg cccggaaaat taccagatta tgtcgtgccg tgccgtgcat aggtctcgga
     1681 gcggcagcgc ttttatgcga gcttcgtatt gaaaatcagc gcaagtgtga actgcgcagc
     1741 acaattactc ggcaatcgag ttgaccccgc tggcgccgag cgcccaggcc tccgccaggt
     1801 cgattccgcg cgcaaacacc ggcagcggca cgcaccggca caagccccga agttcttcgg
     1861 ccgacaatgc agcgcgcatc accaagaagt ccgcgccgac gctcgctgca gcggcccctt
     1921 cggcgacgtc gcggcagtag atgccgagca tccccgcatc gccggcggca attcccggct
     1981 ccgcgacccg atagtcgggc gtctcggcct gcgtgagtcg ctccggcaaa cgcagcgccg
     2041 acacaatggg tttatccgcc ggcaacagct ccacgccggc caattcgttc tgggtgcacc
     2101 agcgcagcgc ctgtccgtcg agtccttgcg gttcgccgct gtagctcctc accacccaca
     2161 tgtcgagaag gatctcgcgc gatggatagg cgtggcgtat gcgcatcaac ggtctggggc
     2221 tcccgatctg gatcccgagc tcttcattga gttctcgcgc caatccggca cggcgctcct
     2281 cgccgacctc gagcttaccg ccgggaaact cccagccgcc cgcgaggtgc acgcctcgcg
     2341 gccgttgcgc gatgagtacg cgatctgccg cgtcgatcac cgctgcggcg acgacgtgaa
     2401 cggtcgggcg cgaaccgata cgactcaacc gctcgcttgc tgcaaagcgc cgtgacagtg
     2461 cttgtattta cggccggacc cgcaagggca gggttcgttg cgcccaacct tgggcaatgg
     2521 gcgcacgaat ggcgcggcgg cggcggcagc gcgcgggtcc atctccatga cgttgcccgg
     2581 agccgcgacg acttcgccgc cgccgaattc gcccggggca ggcgtcgcct ggatgggtga
     2641 aatcgcttcg gcgtgttgcg cctgcagggc ccgcgcgagg cgctgctggc gctcgagctc
     2701 ttcgcgttca atctcagccc ggctgcgcac ttggatcttc gacaccgtcg tcaacgtgtc
     2761 gaactttatc cggtcgagca tcgccgagaa caattcaaag gcctcgcgct tgtactcctg
     2821 cttcgggttc ttctgcgcat agctgcgcag gaaaatgccc tgccgcatgt agtccatcgc
     2881 ggcgaggtgt tcgcgccaat gttgatcgag ggtgcgcagc atcacttcct tctcgatgta
     2941 gcgcatcact tgcagtccga cgctgttctg cttctggtcg taagcggctt ccagggctgc
     3001 ttcgatcttg ctccgcaagc ccgaaccgtc accggcctct tcggctttga gccactcatc
     3061 gatcggcagc gtgagagcga aatcgcgctc gatggactgc gtcaatccgc ggatgtccca
     3121 ctgttcctcg ggggcgccgc cgggaacata ctggtccacg agttgcgcaa ccacgtcctc
     3181 gcgaatgcct ttgacggctt cggcgacgtc ttcggcgctc atgaggtcgg tgcgttgctg
     3241 gtagatcacc cggcgctgat cgttcgcgac gtcgtcatat tccaagagct gtttgcgggc
     3301 atcgaagtta tgttgttcga cgcgccgctg cgcgcgctcg atttggcgcg tcagcatgcc
     3361 gctttctatc gcctcgccgg ccttcatgcc gacccgggtc atgagcgctt tcatgcgcgt
     3421 cggatcgccg aaaatgcgca tcaaattgtc ttcgagcgac aggtagaagc ggctcgagcc
     3481 gggatcgcct tgccggccgg agcggccgcg caattgattg tcgatgcgcc gcgattcatg
     3541 gcgctccgta ccgacgatgt gcaggccgcc ggcagctacg acttgctcat gccgtttacg
     3601 ccagtcgtcg tgtatttgct tgagctcggc cgcgttggga ttctcgatcg cgtcgatctg
     3661 cgcctgcggg ctgccgccga gcacgatatc cgtgccgcgg ccggccatat tggttgcaat
     3721 cgtgatcgca ccgggacgac cggcttccgc gacgatgtgg gcctcgcgtt catgttgctt
     3781 ggcgttcaac acttcatgcg gaatcttcgc cccttgcagc agcttcgcca gccgctccga
     3841 ggtttcgatc gacgttgtgc cgaccagcac cggttgaccg cgcttcacgc agtcctggat
     3901 gtcctcgaga atcgcctgaa acttatcctg ctcggacaga aacaccagat cggatttatc
     3961 cgcgcggatc atcggtttgt gggtgggtat caccacgact tcgagaccgt agatctgctg
     4021 aaattcgaaa gcctcggtgt cggccgtgcc cgtcataccg gacagctttg cgtacagacg
     4081 gaaatagttt tgaaaagtga tcgaggcgac ggtttggttt tcttcgcgta ccttgacgcc
     4141 ctctttcgcc tcgaccgcct ggtgcagacc atcggaccag cgccgcccct gcatggtgcg
     4201 gccggtgaac tcatcgacga tgatcacttc accctggcgc acgatatact cgacgtcgcg
     4261 cttgtataat gcatgcgcgc gcagcgccgc gttcaagtga tgcatgaggc ggatattggt
     4321 ggcgtcgtac aggctctcgc cttcacgcag caaaccgatc tccgccatca actcctcgac
     4381 cttttggtga ccgtcctcgg tcaacgtcgc ctgcttggtt ttttcgtcga ccgcgtaatc
     4441 acccggtcca tcttcggcct tttgccgcgt caaacgcggt atcagcgcat tgatcttgag
     4501 atacagctcg gtgctttcct ccgccggccc cgaaatgatc agcggcgtgc gtgcctcatc
     4561 gatcaggatc gaatcgacct catcgacgat cgcgaaagac agtttgcgct gcacacggtc
     4621 ctcgagacgg aacgccaggt tgtcgcgcaa ataatcgaag ccgaattcgt tgttggtgcc
     4681 gtaggtgatg tcgcaggcgt acgccgcgcg cttctcatcg ggcgactggg cattcttgat
     4741 gacgccgaca ctgagtccga gaaagcggaa aaccggaccc atccactcgg aatcgcgctg
     4801 cgccaggtat tcgttgaccg taacgacgtg cacgccgtcg ccggtgagcg cattgagata
     4861 cgccggcagg gtggacatca gggtcttgcc ctcgccggtg cgcatttccg caatctttcc
     4921 ggaatgcagc gcgaggccgc cgatcaactg cacgtcgaaa tggcgcaacc ccaacttgcg
     4981 ccgcgacgct tcgcgcacca gggcgaaagc ctccggcacc agatcgttca gcgaggtccc
     5041 ggccgcaaag cgcgccttga gctcgcgcgt tttttcggga aattgtgcat ccggcagcgc
     5101 actgatgccc gcctcgaggg catttacttg tgcgacggtg cggctcaatt ccttaaccag
     5161 gcgctggtta cggctgccaa atacttgggt cagaagcttc cacacgcgag ggagtccttg
     5221 agaaaactgc gtattgtacg gatagaccgg aagtgcggga aatcgtttcg aggtcgaccc
     5281 gcaaaacgcc cgctgcggat ctctctcgag gcctagcgat cctcgccgat aaagcgcaaa
     5341 ggatcgacct gagtaccgtt tttcagcact tcaaagtgca agtgggggcc ggtcgatcgg
     5401 ccggtagagc ccatcaacgc gacctcttgg cccttgcgaa ccatatcgcc ctgcttcacc
     5461 aggacttttt cattgtggca gtaacgggtc gcgaggccat tgccatgatt gatctcgacc
     5521 atttggccga accccgagcg ctcgccggcg aaagtcacca aacccgcggc taccgtggtt
     5581 actttagtgc cctcggggcc cgcaaaatca agtcccttat gcacggccgt gtaaccggtg
     5641 aaaggatccg cgcggcggcc gaaataggag gaaatccagc catcttgcac cggcctgccc
     5701 tccggataga cctgtttgtt gagctctcgc gtcagaatga gattctcgag cactcccaat
     5761 tgctgttcgc ggctcgcgag ctgcgtctgc aggtcatcga ccatcgcgct caagttgggg
     5821 atttgcgcgg gttgcccgtc ggcaccgctc tcggagcctc ccagggcagg gggattgccg
     5881 aagtcgaatt ccccgtcatc gaggttggcc atgcgggtca ggcgtttgcc caaggcgttg
     5941 acccggatga cattcgcgtt catctgtccg acgcgcatgg ccagggcatt caccttctcc
     6001 tgcagcacac gcttgacgtt ctccagttgc gcttgctggc gcatcagctc cgccgaccaa
     6061 ttgccgagtt gattgacgga attgcccgaa ctgtggcgcg cgcccaagcc catgccgacg
     6121 gaaaaagcac cacccacgac cgccagcacc acgagcaccg ctgcagcgac tatgacggga
     6181 tgacgcagat cgaattgcct catccggccc gaatttcggc cgacaaaaat gacattcata
     6241 tgaatgcccc aaacgccgtc atgcttcaaa ccggtgcatc atgtccaact gaagttagca
     6301 aggttgtggc cgagcggagc cagtagacta tgcagaaaac tgactcaaaa caaggatttc
     6361 cgcacggttt tccgatgtcg ttaactacca tattcctttg ctgcgatgca aaatcctaga
     6421 cgcgtcagtg acttattgtc acgacctgga aacaaactca cggcgctcaa gactcgctcg
     6481 aggcagcggg cattggtggt cgagcaagtg cgtgcggcgc tccctgcgcg gctcgcccag
     6541 gcggtgacca gcgccggcat cgatcacg
//
LOCUS       DASWZT010000128         5073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_34815, whole genome shotgun sequence.
ACCESSION   DASWZT010000128 DASWZT010000000
VERSION     DASWZT010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34815"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VGE92_03515"
     CDS             complement(<1..512)
                     /locus_tag="VGE92_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HEY1312915.1"
                     /translation="MTSTSRQGAEPILLAFSGGLDTSFCVPWLKDTYQRPVITVTVDT
                     GGIDAAAARSLAERAAKLGAIEHHLIDARDAYFEQVLKFLIMGNVRRGAVYPLCVGAE
                     RVLQAQTIAQMALSLKTSMVAHGCTAAGNDQVRFEVALRTLAPDLEIIAPVRDRAFKR
                     PEQLKYLEDHR"
     gene            complement(518..991)
                     /locus_tag="VGE92_03520"
     CDS             complement(518..991)
                     /locus_tag="VGE92_03520"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312916.1"
                     /translation="MHAPNRNPPPTDNGNAPARRAALAKIIREQTVGRQTELVAMLRK
                     HGHVATQSSVSRDLRELGVAKLGDRYVLPDASPATSDFSALKQFVSARFTAGTNLTVL
                     KTTIGSAQSVAVAIDSARWPEVVGTISGDDTIFIATAGALEQRQLGERLLAIFGR"
     gene            complement(1127..1411)
                     /locus_tag="VGE92_03525"
     CDS             complement(1127..1411)
                     /locus_tag="VGE92_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HEY1312917.1"
                     /translation="MPKLDEQISTLQGKLSQLKLRQQRLEARKRALSAHRERKLAIRR
                     QILVGAIILAKVEQGELDRQQLHGWLDQALTRADDRALFGLSPTPSYDNV"
     gene            1522..2982
                     /gene="mobQ"
                     /locus_tag="VGE92_03530"
     CDS             1522..2982
                     /gene="mobQ"
                     /locus_tag="VGE92_03530"
                     /inference="COORDINATES: protein motif:HMM:NF041496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HEY1312918.1"
                     /translation="MAIYHLTATVISRGRGQSVTAAAAYRSASALRDERYGRVHNYTR
                     THQVVHSEIMAPEAAPAWVHDREALWNRVEAAERRKDSQLARAIEISLPAELSAAENV
                     ALLRDYIAQEFVSKGMIADFCIRRSDPGNPHAHILLTLREPRPSGFGPKARHWNRKSS
                     LFEWRQAWAERANQHLARAGHSVRIDHRTLEAQQSELTPARQMGVGRGRQADERLPEH
                     IKERIAEQQRIARENGEMIVEDPSVALRALTRYRPSFTRQDMLEFLRSRTADAAQLEA
                     VLAAITESNELVELPAKDGQCARFSSRDLLEAEKSLAKRVATLVKRRGHGAVAAPEMP
                     EPALKLTAAPRAALQYLLGEGDIKAFAATPGEDLNAVLAAARRAWESCGLRIAGAAVS
                     RVAVERLQAASGIESQTLQSQEDEWREGRGLPTQNHVLVVDGAEMIGLKQLERLLAVA
                     DKSHAKLVLIGDALQLAAMRSMSPLQNIVAAVGPTG"
     gene            3062..4051
                     /locus_tag="VGE92_03535"
     CDS             3062..4051
                     /locus_tag="VGE92_03535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY1312919.1"
                     /translation="MTQFSVLDLCPVTQGGSPAQSLRNTLDLAQHAERWGFHRYWLAE
                     HHSMPGIASAATSVVIGHVAAGTSSIRVGAGGIMLPNHSPLVIAEQFGTLESLFPGRI
                     DLGLGRAPGSDQVTARALRRNLASDPDQFPQDVLELIGYFAAPEPGQRVTAVPGAGLK
                     VPLWILGSSLFGAQVAAALGLPFAFASHFAPAMMMQAIDIYRTRFEPSAQLREPRVML
                     GFNVFAAESDTEARFLASSMQQAFVNLRSGRPTQLPPPIEGYESQLPPQFKSLLADVL
                     TCSAVGSPETVRAALAAFIARTRADELMITAQIFDHAARLRSYEIAAAVQKSL"
     gene            complement(4048..>5073)
                     /locus_tag="VGE92_03540"
     CDS             complement(4048..>5073)
                     /locus_tag="VGE92_03540"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEY1312920.1"
                     /translation="QADPIRAPARAQDLTLRHRVLDYRAGDLERRYPKLALEEDFFCN
                     YGYVPRVHHQLMHPRTARTVWTTARAAQAEAVLAYVRERGIVHPREVDAHFAHGKVTN
                     WFGGSSNASTQLLDVMHYRGLLRVARREGGTRLYAPCRTAAGFNATPALDADAARSRF
                     DALVDVLVRKYAPLPARSLTQLVWLLRGGAPQWSEQHREAIARAKQRLGHARIEGVDW
                     YWPPTETPISARWRPDLSVRLLTPFDPLVWDRRRFEIFWGWTYRFEAYTPAPKRKLGY
                     YALPLLWHERVIGWGNVTTADGRLRCSLGYVGGRAPREAAFRAALDAELARMRTFLGI
                     GADARSG"
BASE COUNT          896 a         1627 c         1649 g          901 t
ORIGIN      
        1 cggtgatctt cgagatattt gagttgttcc ggccgcttga aggcgcggtc gcgcaccggc
       61 gcaatgatct cgagatccgg cgccagggtg cgcagcgcca cttcgaagcg cacctggtcg
      121 ttgcccgcgg ccgtgcagcc gtgcgcgacc atgctggttt tcagcgacag cgccatttgt
      181 gcgatcgtct gcgcctgcaa tacgcgctcg gcacccacgc acagcggata gacggccccg
      241 cggcggacgt tgcccatgat gaggaacttg agcacttgct cgaaatacgc gtcgcgcgca
      301 tcgatcagat gatgctcgat ggcgcccaat ttcgccgcac gctcggccaa ggaacgcgcc
      361 gcggccgcat cgatgccgcc ggtgtcgacc gtcaccgtga tcaccggccg ctgatacgtg
      421 tccttgagcc agggcacgca aaacgaggtg tcgagtccgc cggagaatgc caagaggatg
      481 ggctcggcgc cctgccttga agtcgatgtc atagagttta acgtccgaaa atggctaaca
      541 gccgttcgcc cagctgccgc tgctcgagcg ccccggcggt ggcgatgaaa atggtgtcat
      601 cgccggaaat ggtgccgacg acctcgggcc aacgggcgct gtcgatggcg accgcgacgc
      661 tttgcgccga accaatggtc gtcttcagca cggtcaaatt cgtgccggcg gtgaagcgcg
      721 cgctgacgaa ctgcttgagc gcggagaagt cgcttgtggc gggggaggca tcgggcagta
      781 cgtagcgatc gcccaacttg gccacgccga gttcgcgcaa gtcgcggctc acgctcgact
      841 gggtcgcgac atggccgtgc ttgcgcaaca tcgccaccag ttcggtttgc cgtcccaccg
      901 tctgctcgcg aatgattttc gcgagcgcag ctcggcgcgc cggggcattg ccgttgtcgg
      961 ttggcggggg atttcggtta ggtgcgtgca taaaagcgcg cgcattttgc ataaaaatgc
     1021 acggcctgtc aacgcgattc gacgccgaac tcgtcgcgga agcctgccgg gttggcaacc
     1081 ggcgttgttc ggaggcagca agccgtagga ccggcttatg ccgcgcttat acgttgtcgt
     1141 aacttggggt agggctcaat ccaaacaacg cccgatcgtc cgcccgcgtg agcgcttggt
     1201 ccaaccagcc atgcaattgc tgacggtcga gctctccttg ttcgaccttc gcgaggatga
     1261 tggcgcccac gagtatctgg cggcggatcg caagcttacg ttcgcgatgc gcgcttagcg
     1321 cgcgtttgcg cgcctccaag cgttgctgac gcaatttcag ctggctgagc ttgccttgga
     1381 gagtcgaaat ctgttcatcg agtttaggca tagagatcga atccgccgcg gtgggaaata
     1441 attgattctc gcacggccgc acgtgtacag tcgatttgca atgaggcagt cccgcactta
     1501 tacatttacg ctcgggtcgg cgtggcgatc taccatttaa cggcgacggt catttcgcgc
     1561 gggcgtgggc aaagcgtgac cgcggcggcg gcgtatcgct ccgcgtccgc gctgcgcgat
     1621 gagcgctacg gccgggtcca caattacacg cgcactcacc aagtcgtgca ttcagaaatc
     1681 atggcacccg aggctgcccc cgcctgggtg cacgaccggg aagcactgtg gaaccgggtc
     1741 gaggccgccg agcgccgcaa agattcgcag ctggcgcgcg ccattgaaat cagcttaccc
     1801 gcggagctgt cggccgccga gaacgtcgcg ctgctacgcg actacattgc ccaggaattc
     1861 gtctcgaaag gcatgatcgc ggacttctgc attcgccgct ccgatccggg taatccgcat
     1921 gcccatatac tgctgacgtt gcgtgaaccc aggccgtcgg gattcgggcc gaaggcgcgg
     1981 cattggaatc gcaaatccag cttgttcgaa tggcgtcagg cgtgggccga gcgggcgaac
     2041 cagcatctgg cgcgcgccgg ccacagcgta cggatcgatc atcgaaccct ggaagcgcaa
     2101 cagagcgagc tcacgccagc gcgtcaaatg ggcgtcgggc gcggccggca ggccgatgag
     2161 cggctaccgg agcacatcaa agagcgcatc gccgagcaac aacggattgc gcgagagaac
     2221 ggcgagatga tcgtcgagga tccgagcgtc gccctgcgcg ctctaacgcg ctaccggccg
     2281 agcttcacgc gtcaagacat gctcgagttt ctgcgctcgc gcaccgccga tgccgcgcag
     2341 ctcgaggcgg tgctcgccgc gatcacggaa tcgaatgagc tggtggagct gcccgcgaag
     2401 gacggccagt gcgcgcgctt ctcgagccgc gacctgctcg aggcggaaaa gtcgctggca
     2461 aagcgggtgg cgacactggt taaacggcgc ggccatggcg ccgtggccgc gcccgagatg
     2521 ccggagcccg ctttgaagct caccgcggcg ccgcgcgccg cgcttcaata cctgctcgga
     2581 gagggcgaca tcaaggcgtt cgccgccacg ccaggcgagg acctcaacgc agttctcgcg
     2641 gcggcccgcc gtgcgtggga atcatgcgga ttgagaatcg ccggcgccgc cgtttccaga
     2701 gttgcggtcg agaggctgca ggcagcgtcc ggcatcgagt cgcaaacgct gcagtcgcaa
     2761 gaagacgagt ggcgcgaagg tcgcggcctg ccgactcaaa accacgtgct cgtggtcgac
     2821 ggcgccgaga tgatcggcct caagcaattg gagcgcctgc tcgcggtcgc ggacaagtca
     2881 cacgccaagc tggtcctcat cggcgatgcg ctgcagctcg cggcgatgcg ttccatgtca
     2941 ccgttgcaaa acatcgtggc ggcagtcggg ccgacaggct agaccggcgc cgcctgcggc
     3001 ggccttgatt atcgggcata cgctccccag agaggtatcc ttccggccgc actgtgagcc
     3061 tatgacccag ttttccgttc tcgatctatg cccggtcacg cagggcggta gtccggcgca
     3121 gtcgttgcgc aacacgctcg atctcgcgca gcacgcggaa cgctgggggt ttcatcgtta
     3181 ttggctggct gagcatcaca gcatgcccgg gatcgcgagc gccgccactt cggtggtgat
     3241 cggtcacgta gccgcaggga cgtcgagcat ccgggtcggt gccggcggca tcatgttgcc
     3301 gaatcattcg ccgctggtca tcgcggagca atttggaact ttggagagcc tgtttcccgg
     3361 ccgcatcgat ctaggcctgg gaagggcccc cggttccgat caagtgacgg cgcgcgcatt
     3421 gcgccgcaat ctcgcctccg atccggacca gtttccgcaa gatgtgttgg agctgatcgg
     3481 atacttcgcg gccccggagc ccggtcagcg cgtcacggcc gtccctggcg ccggactgaa
     3541 agtgccgctg tggatcttgg ggtcgagcct gttcggcgcc caggtggcgg cggcactcgg
     3601 cctgcccttc gccttcgcgt cgcactttgc acctgccatg atgatgcagg ccatcgacat
     3661 ttatcgcacc cggttcgagc cctcggccca gttgcgtgaa ccccgggtga tgttgggatt
     3721 caacgtattt gccgccgaat ccgacaccga agctcggttc ctcgcctcat cgatgcaaca
     3781 agcattcgtg aatctgcgca gcggccggcc gacccaattg ccgccgccga tcgagggata
     3841 tgagagccag ttaccgccgc agttcaagtc gcttctcgcc gatgtcttga cgtgttctgc
     3901 tgtcggctcg cccgagaccg tgcgtgcagc acttgcggcc ttcatcgcgc gcacgcgagc
     3961 tgacgaattg atgatcaccg ctcagatttt cgaccacgcg gcgcggctgc gctcctacga
     4021 aatcgccgcc gccgttcaaa aaagccttta gccgctgcgc gcatccgcgc cgatgccgag
     4081 aaatgtgcgc atgcgcgcaa gttctgcgtc gagagcagca cgaaacgccg cctcgcgcgg
     4141 cgcacggccg ccgacgtagc ccaatgagca gcgcaaccgg ccgtcggcgg ttgtcacatt
     4201 gccccagccg atgacgcgct catgccacag caggggcagc gcgtagtagc cgagcttgcg
     4261 tttcggcgca ggcgtatacg cctcgaaacg gtaggtccag ccccaaaaga tctcgaagcg
     4321 gcgtcggtcc catacgaggg gatcgaacgg cgtgagcagg cgtacgctta agtccggccg
     4381 ccagcgcgcc gagatcggcg tttcggtagg tggccaatac cagtcaactc cttcgatgcg
     4441 cgcgtgaccc agccgttgtt tggcgcgcgc aatcgcctcg cggtgttgct cgctccactg
     4501 cggcgctccg ccacgcaata gccacacgag ttgagtcagg cttctcgccg gcagcggcgc
     4561 atacttgcgg accaagacgt ccaccagcgc gtcgaagcgc gacctcgcgg catccgcgtc
     4621 gagcgccggc gtcgcgttga acccggccgc agtcctgcag ggtgcgtaca ggcgggtgcc
     4681 tccttcgcgg cgtgcgacgc gcagcagccc gcgatagtgc atgacgtcga gcaactgcgt
     4741 gctcgcgttc gaggaaccgc cgaaccaatt ggtcactttg ccatgcgcaa aatgcgcgtc
     4801 cacttcacgc gggtgcacga taccgcgctc gcggacgtac gccaggacgg cctcggcctg
     4861 ggcagcccgc gccgtcgtcc ataccgtgcg ggcggtccgg gggtgcatca actgatgatg
     4921 cacccggggc acatagccgt aattgcaaaa gaagtcctcc tcgagcgcga gcttcggata
     4981 gcgccgttcg aggtcgccgg cccgataatc cagcactcga tgccgcagcg tcaggtcttg
     5041 agcgcgggcc ggggcgcgga ttggatcggc ctg
//
LOCUS       DASWZT010000129         6202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_83128, whole genome shotgun sequence.
ACCESSION   DASWZT010000129 DASWZT010000000
VERSION     DASWZT010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6202
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83128"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1325)
                     /locus_tag="VGE92_03545"
     CDS             complement(156..1325)
                     /locus_tag="VGE92_03545"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY1312921.1"
                     /translation="MNITLLNAQLSAGNITSLRIVGPLIGALGVAAEPGDSIVDLEGD
                     RVLPGLINAHDHLHLNSLARPDFGKSYRHVRDWIADVDARRRSDPEFKASVAVARDER
                     LLIGGVKNLLSGVTTVAHHDPLYPYLANPHYPIHVVRDYGWAHSLDVDGEDKVRRSHR
                     STPSEAPFIMHAAEGVDAAAGHEFERLEALGCLGASTLLVHGVALGRAQRERLAHAGA
                     GLLWCPSSNLNLFGQTAAVSELAARGRVALGTDSRLSGARDLLEELRLVARLGVLGES
                     SLEAIVTSASARLLRLRDRGELTPQARADILVLPARTRLGAARRADVRLVLIDGMIRY
                     GDRDYAERLAPRAALTEVRVDGRAKVLDRNTAALISHAQVGEAGLELAPLNWRAA"
     gene            complement(1322..2029)
                     /locus_tag="VGE92_03550"
     CDS             complement(1322..2029)
                     /locus_tag="VGE92_03550"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY1312922.1"
                     /translation="MQDLCVFDVAGGVSAQRTAETLAAYERWAPLYPATAHNPLMRAE
                     QHAMLAHWPISTGRRALDLACGSGRYSRLLAETQPAHIVAMDFCVPMLRQVSAATRVC
                     GSMMQLPFAARAFDVVISGLALGHADSVHSWMAEVARVLDRGGTLLYSDFHPEAARAG
                     LTRSFKDDKNDTCTVPHHCFELALQQEAAATAGLTIDVVHTVRVGVELREPFDKSEEF
                     YRRWDGLPIVLVVRAGN"
     gene            complement(1990..2991)
                     /locus_tag="VGE92_03555"
     CDS             complement(1990..2991)
                     /locus_tag="VGE92_03555"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HEY1312923.1"
                     /translation="MSASTIAVPRHSGKLPLVTLYLTHRCNSRCLTCDYWRHGRDDMN
                     LASVMRVLPSFAQLGTQWVLLSGGEPLLNPEWAAIGEVLRSHGLSLWLLTSGLALAKH
                     APRVVELIDAITVSLDGTDRATYAAIRGLDAFDKVCEGIRSCADRGKAPAIRVTLQRA
                     NYRQLPAFVDLAKRLGALQVSFLAVDVVNPQAFGRADDFVSDLALRSEDLSVFEQSVA
                     SLERDHAEDFRSGFIAECPEKLRRIHQYFAALRGLGPYPPVRCNAPEFSAVVGATGRV
                     QPCFFIAGPLDAQLAAAENRDLAAVWGSAGMTALRTSIREGHRAECKTCVCSMWRAA"
     gene            3170..3796
                     /gene="ccmA"
                     /locus_tag="VGE92_03560"
     CDS             3170..3796
                     /gene="ccmA"
                     /locus_tag="VGE92_03560"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF010061.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HEY1312924.1"
                     /translation="MNTTAGLAVEKVHVWRGERHVLKGLSLTLAAGQLLHISGPNGSG
                     KTTLLRVVCGLLRPELGLVCWRGRPISSVRAEYQATLAFASHEPALKADLTALENLHF
                     AVGLKRRTTLKGLRDSLQRTGVAECCGLPARVLSAGQRRRVAMARVLAMDAALWVLDE
                     PFTNLDAAGMELFGGLLRSHVQNGGAALVVAHHDLNLGIDVRRLELVP"
     gene            3793..4473
                     /gene="ccmB"
                     /locus_tag="VGE92_03565"
     CDS             3793..4473
                     /gene="ccmB"
                     /locus_tag="VGE92_03565"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEY1312925.1"
                     /translation="MTVGALAAFGLVVRRELRLSFRRPEQLLQPLVFFLIVTTLFPLG
                     LSAQLSLLRDMSPGVLWVAALLSSLLSLDSLFKSDADDGTLEQLALCGQDLALIVVAK
                     TLAHWLVSGAALVLVSPVIGTALGIPVAAFATMMLSLGLGTLTLSWLGAIGAALTVGL
                     KRGNVMLSLIVLPLAMPLMIFGAGATDRAISGASALGALYFLAALCVITCTLAPFAAT
                     VALRITLE"
     gene            4489..5223
                     /locus_tag="VGE92_03570"
     CDS             4489..5223
                     /locus_tag="VGE92_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HEY1312926.1"
                     /translation="MWTWFYKLASPPYVYATAAAFVPWTLGAAALALGYGLIAGLAIA
                     PPDYQQGEAFRMIYVHVPSALLSMTVYAVMATASAVALIWRIKVGHAVAAAAAPIGAS
                     FTLLALVTGSLWGRPMWGTYWVWDPRLTSELILLFLYVGVMSLRSAFEDPSRGDRAAA
                     LLATVGVVNLPIIHFSVVWWNSLHQGATIVKLGAPSMAGSMLWPLLSMWLGFMLFFAA
                     VLCIRVQGEVLNRERLSTWVREAVKS"
     gene            5390..5839
                     /gene="ccmE"
                     /locus_tag="VGE92_03575"
     CDS             5390..5839
                     /gene="ccmE"
                     /locus_tag="VGE92_03575"
                     /inference="COORDINATES: protein motif:HMM:NF009729.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEY1312927.1"
                     /translation="MTPRRKRLFVVLGMLGGVAASVSLAVMASRENIMFYYDPSQVVA
                     GKAPAAKRFRIGGMVVKGSVERKPGDLQVRFVLSDFAHEVPVEYTGVLPDLFREGQGI
                     IAHGTMNAKGAFVADEVLAKHDEKYMPPEVAASLKNKAAATTKAPIG"
     gene            5858..>6202
                     /locus_tag="VGE92_03580"
     CDS             5858..>6202
                     /locus_tag="VGE92_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmF"
                     /protein_id="HEY1312928.1"
                     /translation="MAAELGIFSLILAFVLSLAQALFGLAGAWRGKPVWMAVARPAVT
                     GQFVFVAMAFACLVYCFVHNDFSVLYVARNSNSQLPMFYKVAALWGAHEGSLLLWILI
                     LSIWSVAVAAFSR"
BASE COUNT         1074 a         1844 c         2045 g         1239 t
ORIGIN      
        1 cgcgtccgcg tcgaggcgag catcgacgat cacgacttca tagaggtcgg gttcgagctc
       61 ggagccgatc gcgagcaacg ccaagggcat ggtgaagaac accgcctgcg gattgtagag
      121 cacgaccttg cgccgcgtgc cgctggtggc cgaggtcacg ctgccctcca gtttagcggg
      181 gcaagctcga gtcctgcctc gccgacttgc gcgtgagaga tgagcgcagc cgtgttacgg
      241 tcaagaacct tcgctcgacc atcgacgcga acctcggtaa gggctgcgcg cggcgccagg
      301 cgctcggcat agtctcgatc tccatagcgg atcatgccgt cgatcaatac cagccgcaca
      361 tccgcgcgcc gtgccgctcc caggcgtgtt ctcgccggca gcaccaggat gtctgcgcgg
      421 gcctgcggcg taagttcgcc gcggtcgcgc aatctgagga gccgagcgct ggcgctcgtg
      481 acgatcgctt cgagtgaact ctcgcctagc acgccgagtc gagcgaccaa gcgcaactct
      541 tcgagcaagt cgcgcgcgcc gctcagccgc gaatcggtgc ccaaggcgac gcggccccgc
      601 gcggcgagtt ccgacaccgc cgcagtctga ccgaacaagt tgaggttcga cgacgggcac
      661 caaaggagac ccgcgcccgc gtgcgcgagg cgttctcgct gtgcccgccc gagcgcaacg
      721 ccgtggacca acagggtgct ggcgccgaga cagccgagcg cttcgagacg ttcgaattca
      781 tggccggcgg cggcatcgac gccttcggcc gcatgcatga tgaagggcgc ctcgctcggc
      841 gtcgaccgat gcgaacggcg cactttgtcc tcaccatcca catcgaggga gtgggcccat
      901 ccgtagtcgc ggaccacgtg gatcggataa tgcgggttcg ccaaatacgg atagagcggg
      961 tcatgatgtg caacggtcgt gaccccgctc agaaggttct ttacgccgcc gatcaataag
     1021 cgctcgtcgc gcgcaacggc cacgctggct ttgaactccg gatcggatcg acgacgcgca
     1081 tccacgtctg ctatccagtc gcgcacatga cgatacgatt tgccgaaatc gggacgggcg
     1141 aggctattga ggtgtagatg atcgtgcgca tttatgagac ccggaagcac gcgatcgcct
     1201 tcgaggtcga cgatcgagtc gcccggctcg gccgcaacac ccaaggcgcc aatcagcgga
     1261 ccgacaatcc gtaggctggt gatgttgcct gcgctcaatt gcgcgttcag cagggtgatg
     1321 ttcaatttcc ggcccttacc accagcacga tcggcaggcc gtcccagcgt cgataaaact
     1381 cctccgattt gtcgaatggc tcgcgcaact ccacacccac tcgcaccgtg tggacgacat
     1441 caatcgtcaa gccggccgtg gccgcggctt cctgctgaag cgccaactcg aagcagtggt
     1501 gcgggactgt gcacgtgtca ttcttatcgt ctttgaatga gcgagtgagg cctgcgcgag
     1561 ccgcctcggg atgaaaatcc gaatacagca gcgtgccgcc ccgatcgagt acacgggcga
     1621 cctccgccat ccatgagtgg acgctgtcgg catgaccgag agcgaggccc gatatcacga
     1681 cgtcaaacgc ccgagccgca aatggcagct gcatcatgct accgcagacc cgggtggcgg
     1741 ccgacacctg gcgcagcatg ggaacacaaa agtccatggc cacgatgtgc gcgggctgcg
     1801 tctcggccag caatcgagaa taccgcccgg aaccgcaagc gaggtcgagt gctcgtcgac
     1861 cggtcgagat tggccaatgc gcgagcatcg cgtgttgctc cgcgcgcatg agggggttgt
     1921 gcgccgttgc cggatacaac ggggcccaac gctcgtaagc ggccagcgtt tccgcggtgc
     1981 gctgcgcgct cacgccgccc gccacatcga acacacacag gtcttgcatt cggcccggtg
     2041 gccctcgcga atcgaagtgc gcagtgcagt catgccggcg ctgccccaca cggcggcaag
     2101 gtcacggttt tcggcggccg cgagttgtgc gtccaatggt cctgcaatga agaagcacgg
     2161 ctgcactcgg ccggttgcgc cgacgacggc ggaaaattcc ggtgcattgc agcgtaccgg
     2221 cgggtacgga cccagcccgc gaagcgcggc aaaatattga tggatgcggc gcaacttctc
     2281 ggggcattcc gcgatgaagc ctgatcgaaa atcctcggcg tgatcccgct cgagcgaggc
     2341 cacgctttgt tcgaacaccg ataagtcctc tgagcgtaat gccagatcgg agacaaaatc
     2401 atccgcgcgg ccgaatgcct gcggattgac gacgtcgacc gcaagaaagg agacttgcag
     2461 cgcgcccaag cgctttgcca ggtcgacgaa cgccggcagt tgccggtagt tggcacgctg
     2521 cagagtgacg cgaatcgcag gtgctttgcc gcggtcggcg caagagcgga tgccttcgca
     2581 cactttgtcg aaagcgtcca agccgcgaat cgcggcatag gtggcgcgat cggtcccatc
     2641 caacgacacc gtgatggcgt cgatgagttc gaccacacgc ggcgcgtgct tggccaacgc
     2701 caggcccgaa gtgagcagcc acaacgaaag cccgtggcta cgcagtacct cgccgatcgc
     2761 cgcccattcg gggtttagga gcggttcgcc gccggacaaa agtacccatt gcgtgcccaa
     2821 ttgcgcgaag ctgggcagaa cacgcatcac cgaggcgaga ttcatatcgt cgcggccatg
     2881 gcgccagtag tcgcatgtga ggcagcggga gttgcagcga tgcgtcaaat acagcgttac
     2941 cagaggcagt ttgcctgaat gccggggaac tgcgatggtg ctggcggaca aattgcgact
     3001 cttgagatga agttacacgc gctcgacaaa tacaccgaag gattcgctca tgagtacccc
     3061 ggaggagcat ttcggtttaa ctgcccgacc cgaaattgcg cgcttcatgt cgccggtcgc
     3121 gcatgaaaaa gtagcttcaa ccctttgatt tagttaagct cgcggcctca tgaatacgac
     3181 agcaggactc gcagtcgaga aggtccacgt ttggcgcggt gagcgtcatg tgctcaaggg
     3241 cttgagtttg acgttggcgg cgggccaact gctgcatatc tcggggccga acggcagcgg
     3301 caagaccacc ctgctgcgcg tggtgtgcgg cctgctgcga cccgagttgg gcttggtgtg
     3361 ctggcgggga cggccgattt cgagcgtcag ggccgaatat caagccactc tggcgttcgc
     3421 ttcgcatgaa ccggcgctca aggccgattt gaccgcgctc gagaatctgc actttgccgt
     3481 ggggttgaag cgccgcacca cgcttaaggg cttgcgtgac agcctgcagc gcaccggcgt
     3541 cgcggaatgc tgcggtttgc cggctcgcgt gctgtcggca ggccagcggc gacgcgtcgc
     3601 gatggcgcga gtgctcgcga tggacgcggc tctctgggtc ctggatgagc cgttcactaa
     3661 tttggatgcc gcgggcatgg agttgtttgg cggcttgctt cgctcccacg tgcagaacgg
     3721 aggtgcagca ttggtcgttg cacatcatga tctcaattta ggcatcgacg tgcgccgact
     3781 ggaactcgtg ccatgacggt tggcgctctc gcggcgttcg ggttggtggt gcgtcgtgaa
     3841 ctacgtctca gtttccgtcg tcccgagcag ctgttgcaac ctttggtgtt ctttttgatc
     3901 gtaactacat tgtttcccct gggcttgagc gcgcagctgt cgctgctgcg agacatgtcg
     3961 ccgggtgtgc tttgggtggc ggcgctgcta tcgtctctgt tgtcgttgga ttcgctgttc
     4021 aagagcgatg ccgatgacgg caccttggag cagttggcgc tctgcggcca ggacttggcg
     4081 ctcatcgtgg tggcgaaaac cctggctcat tggttggtca gcggcgcggc gttggttctg
     4141 gtgtcgcccg tgatcggcac cgcgctcgga attccggtgg cggcgtttgc cacgatgatg
     4201 ctgagcttgg gccttggcac gttgacgttg agttggctcg gtgccatcgg tgcggcactc
     4261 accgtggggc tgaagcgcgg caatgtgatg ctgtcgctga tcgtgctgcc gctcgcgatg
     4321 ccgctcatga tcttcggcgc cggcgcgacg gatcgtgcga tttccggggc gagcgcgctt
     4381 ggcgccttgt attttttggc ggcgctttgc gtgatcacct gcacgctggc gccgttcgcg
     4441 gcaacggtgg cgttgcgtat tacgctcgaa tgaggattgt gtaatcgaat gtggacctgg
     4501 ttctataagc ttgcctcgcc gccctatgta tacgcaaccg cggccgcgtt cgtgccttgg
     4561 acgttgggtg ccgcagcgct tgccttggga tatgggttga tcgcgggtct cgcgattgcg
     4621 cctcccgact accaacaggg cgaggcgttt cgcatgattt acgtgcacgt gccgagcgca
     4681 ttgctgtcca tgaccgtgta tgcggtcatg gcgacggcca gtgccgtcgc gctcatctgg
     4741 cgcatcaagg tgggccatgc cgtggcggcg gcggcggcgc ccattggtgc gtctttcacg
     4801 ctgctggcgc tggtcaccgg ttcgctgtgg ggccggccga tgtggggcac ctattgggtc
     4861 tgggacccca ggctcacgtc cgaactcatc ctgttgtttc tgtatgtggg tgtcatgtcg
     4921 ctgcgctcgg cgttcgagga cccttcgcgc ggcgaccggg cggccgcgct gctggcaacg
     4981 gtcggcgtcg tcaacttgcc aatcattcat ttttcggtcg tttggtggaa ctcactgcat
     5041 cagggggcaa cgattgttaa actaggggca ccttccatgg ccggcagcat gctctggccg
     5101 ctgctgtcga tgtggcttgg tttcatgctg ttcttcgccg cagtgctgtg cattcgggtg
     5161 caaggtgaag tgctcaatcg cgaacggctg tcaacgtggg tgcgagaggc ggtcaaatca
     5221 tgataagcgg cagcttttgg tcgatggggg ggtacgcgcg ctatgtgtgg ccctgcttcg
     5281 tatttgcggg cgccgtactc gcgtggaacc tatggtccgc gcggcgctat catgccgcgg
     5341 cgcgggtgcg cgcggcacgt atgctggacg tggcggcagg tgacgaacga tgacgccgcg
     5401 gcgcaaacgc ctgttcgtgg tgctcggcat gctcggcggg gtggccgcgt cggtgtcgct
     5461 cgcggtcatg gcttcgcgcg agaacatcat gttctattac gatccgagtc aagtggtggc
     5521 gggcaaagca ccggccgcca agcgctttcg catcggcggg atggtggtca agggcagcgt
     5581 cgagcgaaag cccggcgatt tgcaggtgcg attcgtgctc agcgacttcg ctcacgaggt
     5641 tccggtagag tacacgggcg tgctgccgga tctgtttcgc gaagggcaag gtatcattgc
     5701 gcacggcacc atgaatgcca aaggtgcctt tgtcgccgac gaggttcttg caaaacatga
     5761 tgagaaatac atgccgccag aagtggccgc ttcgctgaag aataaagccg ccgcgaccac
     5821 caaggcgccg atcggctaag gcctacggaa acatcaaatg gcagcggaac tcggaatttt
     5881 ctccctcatt ttggcatttg tcttgagcct cgcgcaggcg ttgttcggtc tcgcgggtgc
     5941 ctggcgcggc aagcccgtgt ggatggcggt cgcccggccg gcggtaaccg gacagttcgt
     6001 gttcgtcgcc atggcgtttg cgtgtctcgt gtactgcttc gtccacaacg acttctcggt
     6061 gctgtatgtg gcgcgcaatt ccaattcgca actgccgatg ttctacaagg tcgccgcgct
     6121 gtggggcgcc cacgagggct cgctgctgtt gtggatactg atcctgtcga tctggagcgt
     6181 ggcggttgcc gccttcagcc gg
//
LOCUS       DASWZT010000130         2608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_20319, whole genome shotgun sequence.
ACCESSION   DASWZT010000130 DASWZT010000000
VERSION     DASWZT010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20319"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="VGE92_03585"
     CDS             complement(<1..1191)
                     /locus_tag="VGE92_03585"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HEY1312929.1"
                     /translation="MVVIRTTLAPIVAAAALSTALLASMQIRAQTTVASAADMVPLPE
                     RPVIAPIMDCAALTAKDFSRLAEGPARVQSATLEPAAAARAEFCLVKGYVAPTIQFEL
                     RLPTKTWTGRYLQGGCGGNCGVIVNFVAPRCDSIPAFGGAFAVGLENSGHVGGDGVWA
                     LGGQQVREDFAFRAAHAFAAAAKAIIAVFYGQPPAFSYFQGCSDGGREAMVETQRYPS
                     DFNGVIAGSPAFAIAEAMERFIWEARWGKDESGALLIDAAAATLLHEAVLEACDALDG
                     ARDGQIDDPRRCHFDPQALVCHGGRSPPHCLTERQAEVARKFYSGPIDAQGRHLFYGG
                     EPYGSELSWVERYALAAAGGAMFEDAVKNMMFQGSLDADASVGNWRFDLATFRELSRR
                     GAIYD"
     gene            complement(1191..>2608)
                     /locus_tag="VGE92_03590"
     CDS             complement(1191..>2608)
                     /locus_tag="VGE92_03590"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEY1312930.1"
                     /translation="RRKVLRAGTAIGAAALGMPRFGRAADSGVPGISPPLADTATAGV
                     LVWNVDALGYEAHEYFLTGRANVYKPVSMADAPDVASRDNLKDLGRRDFSRELLSAAV
                     PFTTRLIVYRPQDKRRFSGNVIVETLHPNGGGASVVWNAVHDYFAAGGDAYVGVQHPL
                     TMAGLKAADPGRYAQLNAADPTQLWGMLAQAGAAIKTQGASSPLAGYPVKHLLMTGYS
                     YTGVATATFANYHHEEAKLENGRNIFAGYLPMADAQYVKPLDVPVMRLNTQSDFNGFG
                     GLNNRRPDDDRYRHYEIAGASHVAIPPPPGAGVPPAPGKVPTPSGQPHFSPTRCQIEF
                     PAGSHPNDYPLYLVQAALFANMYSWIGGGKLPPPSAMIETNADGSCRLDDYGNALGGV
                     RYPQVAVPVAAYGVGNNDACLLFGYTAPFDAGRCRRIYGNQSNYLSKVRLAGGKLVAD
                     RLLLQDDLSKLVAIARTGASF"
BASE COUNT          494 a          830 c          839 g          445 t
ORIGIN      
        1 gtcatagatg gcgccgcggc ggctcaactc gcgaaaagtc gccaaatcga aacgccaatt
       61 gccgaccgat gcatcggcat cgaggctgcc ttgaaacatc atattcttga ccgcgtcctc
      121 gaacatcgcg ccgcccgcgg ctgccaacgc gtagcgctcc acccaactca gctccgaccc
      181 atagggctcg ccgccgtaga agagatgccg accctgtgca tcgatcggcc ccgaataaaa
      241 ttttcgcgcg acctcggcct gccgttcggt caaacagtgc ggaggcgacc ggccgccgtg
      301 gcaaaccagc gcttgcggat cgaagtggca gcgcctgggg tcatcgattt gcccgtcgcg
      361 ggcgccatcg agtgcatcgc acgcctcgag cacggcctca tgcagaagcg tcgccgcggc
      421 tgcgtcgatc aacaacgcgc cggactcgtc cttgccccag cgtgcttccc agatgaagcg
      481 ctccatggct tccgcgattg cgaacgccgg cgagccggcg atcacgccgt tgaaatcgga
      541 cggatagcgt tgcgtctcaa ccatggcttc gcggccgcca tcggaacacc cttgaaagta
      601 ggagaacgcg ggcggctgac catagaaaac ggcgatgatg gctttggccg ctgcggcgaa
      661 cgcgtgcgcg gcacggaatg caaaatcctc gcgcacttgc tgaccgccga gggcccatac
      721 gccatcgccg cccacgtggc ctgaattctc gaggccgacc gcgaacgcgc cgccaaacgc
      781 aggaatcgaa tcgcagcgcg gcgccacgaa gttgacgatg accccacagt tgccgccgca
      841 gccgccctgc agataccttc ccgtccaggt cttcgtcggc aaccgcaatt cgaattggat
      901 cgtcggggcg acgtagccct tgaccaggca gaattccgcg cgcgcagcgg cggcgggttc
      961 gagcgtcgcg gattggactc gcgcggggcc ttcggccagg cgcgaaaaat ctttggcagt
     1021 cagtgcggca cagtccatga tcggtgcgat gactggcctt tcgggcagcg gcaccatgtc
     1081 cgcggcggat gccacggttg tctgcgcacg gatctgcatc gaggcgagca atgctgtcga
     1141 aagcgccgct gccgcaacga tcggcgcgag agttgtgcgg ataacaacca tcaaaatgat
     1201 gctcccgtcc tcgcgatggc cacgagtttc gagaggtcat cctgcaacaa caaccgatcg
     1261 gcgacgagtt tcccgccagc caatcgcacc ttggacaggt agttcgattg attgccgtag
     1321 atcctccggc accggccggc gtcgaagggc gcggtgtagc caaacaaaag acacgcgtcg
     1381 ttgttgccaa caccataagc cgcgacgggc actgcgactt gcggatagcg cacgccaccc
     1441 agggcattgc cgtagtcatc gagccggcag gagccgtcgg cattggtttc gatcatcgcg
     1501 gagggcggcg ggagcttgcc cccgcctatc caactgtaca tattggcgaa cagggcggcc
     1561 tgaaccaggt aaagaggata gtcgttggga tgcgagccgg cagggaactc gatctgacat
     1621 cgggtcggtg agaaatgcgg ctgtccgctg ggcgtcggta ccttcccggg tgccggcggg
     1681 acgccggcgc cgggcggggg cgggatggcg acatgggagg cgccggcgat ctcgtaatga
     1741 cggtatcgat cgtcatcggg gcggcgatta ttcagcccgc cgaaaccatt gaagtcgctt
     1801 tgcgtattga gccgcatgac gggaacgtcc agcggcttga cgtattgcgc atccgccatc
     1861 ggcaggtagc cagcgaagat gtttcgtccg ttctcgagct tggcctcttc gtgatggtag
     1921 ttggcgaaag tggcggtggc cacgccggta tacgaatacc ccgtcatcaa gaggtgtttg
     1981 accgggtaac ccgcgagggg actcgaagca ccctgcgttt tgatggctgc gccggcctgc
     2041 gccagcatgc cccacaattg cgttgggtcg gcggcgttca attgggcgta gcggcccgga
     2101 tccgcggcct tgaggcccgc catcgtcaga gggtgctgca cgcccacata ggcgtcgccg
     2161 ccggcggcaa aataatcatg aacggcgttc cagaccacac tcgcgccgcc accgttggga
     2221 tgcagcgttt ccacgatgac gttgccgctg aagcgtcttt tgtcctgggg ccgatagacg
     2281 atcaaacgcg tggtgaacgg cactgcggcg ctgagaagtt cgcgcgagaa gtcgcgccgg
     2341 cccagatcct tgagattatc ccgcgaggca acatccgggg cgtccgccat cgacacaggt
     2401 ttgtacacgt tggcacggcc cgtcaggaaa tactcgtgcg cctcgtaccc cagggcgtcg
     2461 acgttccaaa cgaggacccc tgcggttgca gtatccgcca ggggcgggga tatgcccggc
     2521 acgccggagt ccgcggctcg gccgaagcgc ggcatgccta gcgccgccgc gccgattgca
     2581 gttcccgccc gcaggacttt gcgccgca
//
LOCUS       DASWZT010000131         1503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_20323, whole genome shotgun sequence.
ACCESSION   DASWZT010000131 DASWZT010000000
VERSION     DASWZT010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20323"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VGE92_03595"
     CDS             <1..704
                     /locus_tag="VGE92_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312931.1"
                     /translation="LIGCMMGNPFINAVYIGHPGWKAMGGRLGYSAATGIMVILLCWF
                     GTISVISAIIPSIAILPILLYIGMLIGSQAFQETPKSHAPAIILAVVPSIANWGLTMI
                     NNSLAAAGILTVSDQLASGMLNQGVFYRGLQILGGGSTLGGLVLGATAVCIIDRRLFK
                     AAGFALTGAVLTFFGLMHGEHIGIAQSPQVSSAYLLVSALLAGCAKYAAPAPADIAEY
                     PGAEGETEAAAAGAS"
     gene            complement(1244..>1503)
                     /locus_tag="VGE92_03600"
     CDS             complement(1244..>1503)
                     /locus_tag="VGE92_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312932.1"
                     /translation="VPIISLRVSVFSANSFPEFFILSPYKLLTVVIVIAPCTLESSPN
                     AYARLIVARIVPLTAGSAPSTYGVRRSVYWYVGVSGAWTTW"
BASE COUNT          314 a          442 c          445 g          302 t
ORIGIN      
        1 gcctcatcgg ctgtatgatg ggcaacccgt tcatcaacgc ggtttacatc ggccatcccg
       61 gctggaaagc gatgggcggt cgtctcggct actccgcagc caccggcatc atggtgattc
      121 tgctctgctg gttcggcacc atctccgtga tatcggccat cattccgagc atcgccattt
      181 tacccattct gctttacatc ggtatgttga tcggttcgca agcattccag gaaacgccca
      241 aaagccatgc gcctgccatt attctggcag tcgttccttc gattgcgaac tgggggttga
      301 ccatgatcaa caactcgctc gcggcggcgg gcatcctgac ggtgtccgac cagcttgcca
      361 gtggcatgct gaaccaggga gtcttttatc gcggcctgca aatactgggc ggcggctcaa
      421 ccctgggcgg attggttctg ggagcgaccg cggtgtgcat catcgaccga cggctgttca
      481 aggccgccgg ctttgcgctc accggggccg tcctgacgtt ttttggcctg atgcacggcg
      541 agcacatcgg catcgctcaa tcgccgcagg tttcaagcgc ttatctgctc gtctcggcgc
      601 tgctggcggg gtgcgccaaa tatgctgccc cggctccggc cgacatcgcc gaatatccag
      661 gagccgaggg cgagaccgag gccgccgctg cgggcgcgag ttagcctggc ggggcgaaag
      721 gcgttactca ggcgcggtcg aggtggtccg caaaagcctt gcgccccggc tccgatgacc
      781 gtacgttgcc cgcggcagat aattgcccga ctgagattcc acttcgattc gccggccgcg
      841 cgcgctgctg cgccaacagc gtcggagaca acaggaaaca aggtgcctgg ccacaatagg
      901 tgcttaaacg ttttgggggt tcggcccgtt ttacgtaaga cagcaccgct cgctgacgcg
      961 cgcggctcag caagtgctct gaaatcaata ccaacattcc gagccgcgac cgtaaggaag
     1021 cggtacacag tcttcggaat gaccgatttc cgctggaacc cccaaaatgg ttaagcaccc
     1081 caatcgatat tgttgctata tactactcgg gaactcagag gaggaatatg gcgcaggcga
     1141 ttaacatcgt tagaaccgcc gtcggagtat ctttcagtcc ggcgcagttg gcagtcgaaa
     1201 caggtgcggc cgtcttttgg gtaaacctgg acccacaagc ggctcaccag gtggtccagg
     1261 cgcctgacac accgacgtac caatatacgg atctcctgac gccatacgtc gaaggagcgg
     1321 agccggcggt cagcggcacg atcctcgcga caattaacct tgcgtacgcg ttgggtgaag
     1381 attcgagcgt gcaaggtgcg atcactataa ccacggttag caacttataa ggggacagta
     1441 tgaagaactc cgggaaggaa ttcgccgaaa aaacactcac gcgcagagag atgatcggaa
     1501 cgg
//
LOCUS       DASWZT010000132         2123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_30072, whole genome shotgun sequence.
ACCESSION   DASWZT010000132 DASWZT010000000
VERSION     DASWZT010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30072"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="VGE92_03605"
     CDS             complement(<1..1093)
                     /locus_tag="VGE92_03605"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEY1312933.1"
                     /translation="MTRAALIVRDVHKRFPGVKALDGVDLEVRAGEVHALLGENGAGK
                     STLLKILSGAQRADRGEAALDGVTLDPDASPRERQRLGLITIYQEFNLLPFMSIAENL
                     YLGREPVRRGLIDWAAMYDNARALLANLGLDADPRREVRHLSVAHQQMVEIGRAVAQQ
                     AKLIVMDEPTAALSGREVDILHGVIRELQARGVSFIYVTHRLDEVKQVCDSYTVLRDG
                     RFVARGEVKDVQIRDLINLMVGRDVEFARLARTAPRGKVVLAVEGVSRACEAGGRHAT
                     ALQDLSIDLKAGEILGFAGLVGAGRTELARIIFGADRCDRGVLYLEGLKMRPLRSPQE
                     AIRAGFALVPEDRKQQGCFLEQSITQNMTL"
     gene            complement(1093..2064)
                     /locus_tag="VGE92_03610"
     CDS             complement(1093..2064)
                     /locus_tag="VGE92_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY1312934.1"
                     /translation="MIIKSRLLVGARLSAALLLTGAMASAGCSGKAGADSIQIVASLH
                     DMTEPFFVAVRRQLDTEAKRLHVTVSVEDGQSNSAKQTADIEAAIAGGAQGIILAPND
                     VNALAPAVEEVIKAGVPIVTLDRRVDHTSVAVAHVGADNVAGGRIMAQWVIDSFPNGA
                     RILLITGQLGSSPAIDRTRGIKETLAAAGDKYKIVAEQSATWKREQGLTVTQNVLTSL
                     GGQSLQAIVSESDEMALGALEALRSSGVSGVKVIGFDATPEALKLVRSGEMAATVEQS
                     PIRQAQTALRQLVAHLRDKTPITGASIAPVLITQASIEQAERIGEVH"
BASE COUNT          358 a          711 c          659 g          395 t
ORIGIN      
        1 gcagcgtcat gttctgcgtg atcgattgct cgagaaaaca tccctgctgt ttgcgatctt
       61 cgggcaccag ggcaaatccc gcgcgaatgg cctcctgggg cgatcgcagc gggcgcatct
      121 tcaggccctc gaggtacagc actccccgat cgcaccgatc cgccccaaaa atgatgcgcg
      181 caagctccgt gcggcccgcg ccgaccaagc ctgcaaaccc gaggatttca cccgctttca
      241 agtcgatcga caaatcttga agcgcggtcg catgccggcc gccggcctcg cacgcgcgcg
      301 acacaccctc gaccgcgagc accaccttgc cgcgcggcgc cgtgcgcgca aggcgcgcga
      361 attcgacatc gcggcccacc atgagattga tcaaatcgcg aatctgcaca tccttgacct
      421 cgccgcgggc gacgaagcgg ccatcgcgca gcaccgtata gctgtcgcaa acctgcttga
      481 cctcatccaa ccggtgcgtg acgtagatga agctcacgcc gcgtgcctgc aattcccgga
      541 tcactccgtg caggatatcc acctcgcgcc cgctcaaggc cgcggtcggt tcgtccatca
      601 cgatcagctt cgcctgttgc gcgacggcgc gcccgatttc gaccatttgt tgatgggcga
      661 cgctcaaatg ccgcacctcg cgccgtggat cggcgtcgag gcccaagttc gcgagcagcg
      721 cgcgtgcgtt gtcgtacatg gccgcccaat cgatgaggcc gcggcgcacg ggttcgcgcc
      781 ccaagtacag gttctcggcg atcgacatga agggcagtaa attgaattcc tgatagatgg
      841 tgatgaggcc gaggcgttgc cgctcgcgcg gcgaggcgtc ggggtcgagc gtcacgccgt
      901 cgagcgcagc ctcgcctcga tcggcgcgtt gcgcgcccga gagaatcttg agcagggtgg
      961 attttcccgc gccgttctcg cccaacagcg cgtgcacttc tcccgctcgg acctcgagat
     1021 cgaccccgtc caaggccttc acgcccggaa atcgtttgtg gacgtcgcgg acgatgagag
     1081 cggcgcgggt cactagtgaa cttcgccgat gcgttccgct tgctcgatgc tcgcttgggt
     1141 gatgaggacc ggggcgatgc tcgcacccgt gatgggtgtc ttgtcgcgca ggtgcgccac
     1201 cagttgccgc agcgccgtct gcgcctggcg gatcggcgac tgctcgacgg tcgcggccat
     1261 ctcgccgctg cgcacgagtt tcagcgcctc gggggtggca tcgaagccga tgactttcac
     1321 cccggaaact ccgctcgagc gcagtgcctc caaggcaccc aatgccatct cgtcgctttc
     1381 ggatacgatg gcctgcaatg attggccgcc gagcgaggtc aatacattct gcgtcacggt
     1441 gagaccctgc tcgcgcttcc aggtggccga ctgctccgcc acgattttgt atttatcgcc
     1501 cgccgccgcc agggtctcct tgatgccgcg ggtgcggtcg atggcagggc tcgaccccaa
     1561 ctgtccggtg atcaagagaa tgcgcgcgcc gttcggaaac gagtcgatca cccattgggc
     1621 catgatgcga ccgcccgcga cattatccgc gccgacatgc gcaacggcaa cgctggtgtg
     1681 gtcgactcgg cgatccaagg tcacgatggg aacgcccgcc ttgatcacct cttccaccgc
     1741 gggggcgagc gcgttgacgt cgttgggggc caagatgatg ccttgtgcgc cgccggcgat
     1801 cgcggcttcg atgtcggcgg tttgtttggc tgaattggat tgtccgtctt ccaccgagac
     1861 cgtgacgtgc agacgcttgg cctcggtgtc gagttgccgt cgcaccgcga cgaagaaggg
     1921 ctcggtcatg tcatggagac tcgcgacgat ctggatcgaa tcggcgcccg ctttacccga
     1981 gcatccggcg ctcgccatcg cgcccgtcaa cagcagcgcg gccgacaggc gcgcgccgac
     2041 gagcagtctc gattttatga tcatgcttcc cccaaaatcc gcggaaaatc agtcgttcgt
     2101 tgcgcgttag cgcggcgccg ccg
//
LOCUS       DASWZT010000133         3183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_44597, whole genome shotgun sequence.
ACCESSION   DASWZT010000133 DASWZT010000000
VERSION     DASWZT010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44597"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..1182
                     /locus_tag="VGE92_03615"
     CDS             16..1182
                     /locus_tag="VGE92_03615"
                     /inference="COORDINATES: protein motif:HMM:NF020260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312935.1"
                     /translation="MFPVVLLFIEMPTAEVDVNVHPSKTEVRFRQQTVVHDFVRESVR
                     AALMKARPVPQFLKEINAQPSATPGLSSPGRGGARFSDGAPATDGFERAPWRDLFAPA
                     TEGGFALQAPAVPAAPGRFEFAGGIAIEGNAALSLARAPEAAIPQHAMDPQPVFAQPV
                     VAPSIAAPNMVAQQIPDHGCAPPIVQELQSAPTLESLRTLRPLGQIRNSFILAVNEDG
                     LWIIDQHVAHERVLFEKILRQRSAQLVESQRLLLPLIVELTPAQHAIFSDIAEELSYN
                     GFEAEPFGARSVAVKIAPAGVDAAQIEHMLNEIFEQLMREDQAINLEAVRTRIAASIA
                     CHAAIKVNMPLEQKKMEWLLAELAKAEHPMSCPHGRPVVLRYSMQDIQKAFKRI"
     gene            1339..2220
                     /locus_tag="VGE92_03620"
     CDS             1339..2220
                     /locus_tag="VGE92_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312936.1"
                     /translation="MTDAELQLARAQKWRLVGEPMRTLDEAVGFLESVGFCLMYPMRP
                     ALLVPTFIGAWVGADDKLPTRQHAYSDPRAKEATELMVRALRAHSAYEAPLLDENNAF
                     LLAPSVFPYFYSLVGERNPKQPPALGPRSPYSPLACDAFEIIRRRGPISKMKLGEMLG
                     GSVSNAGLDRALSELWAKLRITRVNYTVEEGSVWDELSRWAPEVVREGVGISLPEALS
                     ALVSKYLDCVVAVEEGEVENFLANFVPRTRVKEAIHALLAAREVSFVHVGSKSLLQVT
                     PPKQVYIPKNRRPERVS"
     gene            2365..3072
                     /gene="cmk"
                     /locus_tag="VGE92_03625"
     CDS             2365..3072
                     /gene="cmk"
                     /locus_tag="VGE92_03625"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEY1312937.1"
                     /translation="MAAATMPDLSPRKLIIAIDGPAGAGKSTIASRLARKLGYVNLES
                     GAMYRALALRAIEWDASFDDEAALLKMAQDSRITLDRIIGGNRVLLNGKDISARIRER
                     DVTEGASRVSVHPKVREWMVARQREMGLGGGVVMEGRDIGTKVFPDADLKIFLDADPV
                     IREQRRLDQQRVRGAPAESMAAELRERDRRDRTRAASPLVAAEDAVVLDSTHMTEDEV
                     LDRIQELVEQRLAARPA"
     gene            complement(3086..>3183)
                     /locus_tag="VGE92_03630"
     CDS             complement(3086..>3183)
                     /locus_tag="VGE92_03630"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HEY1312938.1"
                     /translation="AHLGHVFDDGPRPTGLRYCMNSASLKFIKRS"
BASE COUNT          584 a          962 c         1032 g          605 t
ORIGIN      
        1 attctgccgc ctgcggtgtt tccggtcgtg ctgctcttta ttgagatgcc gaccgccgag
       61 gtggatgtga atgtccatcc ctcaaagacc gaggtgcgtt tccggcagca gacggtggtt
      121 cacgattttg ttcgcgagtc cgtgcgggcg gcgctgatga aggcacggcc ggtgccgcaa
      181 ttcctgaagg aaatcaacgc ccagccctcg gcaactcccg gcctcagcag ccccggcagg
      241 ggcggcgctc gcttctcgga tggcgcgccc gcgaccgatg gattcgagcg cgcgccgtgg
      301 cgggacttgt ttgcgcccgc gaccgagggc ggattcgcgc tgcaagcgcc ggccgttcct
      361 gccgccccgg ggcgcttcga gtttgccggc ggcatcgcga tcgaaggcaa cgctgcgcta
      421 tcgctggctc gcgcccccga ggctgcgatt ccccagcatg cgatggatcc tcagccggtc
      481 tttgctcagc ccgttgtcgc gccgtcgatt gctgccccaa acatggttgc tcagcaaatc
      541 cccgatcacg gctgcgctcc gcccatcgtg caggaattgc aatccgcgcc aacgctggaa
      601 tcgctgcgaa cgctgcggcc gttagggcag attcgaaatt catttattct tgccgttaac
      661 gaagatgggc tctggattat cgatcagcac gtggcccacg agcgtgtgct gttcgaaaaa
      721 atattgcggc aacgctccgc gcaactggtg gagagccagc gcctgctgct gcctctgatc
      781 gtcgaattga ccccggcgca gcacgccatc ttcagcgaca tcgcggaaga gctttcgtat
      841 aacggctttg aggcggaacc tttcggagcg cgcagtgtgg cggtaaagat cgcaccggcg
      901 ggcgtggatg cggcgcagat cgagcatatg ctgaacgaaa tcttcgaaca gctcatgcgc
      961 gaagatcaag ccatcaatct cgaagcggtg cgaacccgca tcgctgcctc catagcgtgc
     1021 cacgccgcca ttaaggtcaa catgccgctc gaacaaaaga agatggaatg gctgctggcc
     1081 gaactggcga aagccgagca tccgatgtca tgtccgcacg gtaggccggt ggtgctgcgt
     1141 tattccatgc aggacattca gaaggcgttc aagaggatat agctctcagc cgtcagctcg
     1201 cggtagaatc tcgcccattt ggggagttgc tctgctgctg agaaccgaga agcgatgggc
     1261 gatcgcggtg agatcgcgtc caagagccaa gagccaagag ctgaccgctg accgctagcc
     1321 tgtgttctaa tcgaagccat gaccgacgcc gaacttcagc tggcgcgcgc acaaaagtgg
     1381 cgtctcgtcg gcgagcccat gcgcacgctt gacgaagccg tcggctttct cgagtcggtg
     1441 ggattttgcc tgatgtatcc gatgcggccg gcgctgctgg tcccgacttt tataggggcg
     1501 tgggtgggag ccgacgacaa actgcccacc cgccagcacg cttactccga tccgcgagcg
     1561 aaggaagcaa ccgagttgat ggtgcgcgcc ctgcgcgctc actcggctta cgaggctccg
     1621 ctgttggatg aaaacaacgc tttcctgctg gcgccctcgg tgtttcccta tttttattcg
     1681 ctggtgggcg aacgcaatcc caagcagccg ccggcgttgg gaccgcgttc cccgtattcg
     1741 ccgctggcct gcgacgcttt cgaaatcatc cggcggcggg gaccgatctc gaagatgaaa
     1801 ctcggagaga tgctcggagg ctcggtttcg aacgccggcc tcgatcgcgc gctcagcgag
     1861 ttgtgggcga agttgcgcat cacgcgcgtc aactacacgg tggaagaagg atcggtgtgg
     1921 gacgaattat cgcgctgggc gcccgaggtg gtgcgcgagg gcgtgggaat ttccttgccg
     1981 gaagcgcttt cggcgctggt ctccaaatat ttggactgcg tggttgcggt ggaggaaggc
     2041 gaagtcgaaa atttcctggc aaactttgtt ccgcggacgc gggtgaaaga ggcgatccat
     2101 gccctgctgg cggcgcgcga agtgagcttt gtccatgtcg gcagcaagtc cctgctgcag
     2161 gtgactccgc cgaagcaagt gtacattccc aagaatcgcc gcccggagcg ggtgagctga
     2221 gttcgcgggg ccggcgtccc ggcccgtcca gccgacgctg acagcgaacc caactctgcg
     2281 cacctgcctg gtttcccttg tgttccccgg tgccctccgt ggtgaagctc tcgcgtgctc
     2341 cgtgtacaat tctcagttca tccaatggcc gcagccacca tgcccgatct ctcgccacgc
     2401 aagctcatca tcgccatcga cggcccggcc ggagccggca agagcacgat cgcttcgcgc
     2461 ctggcgcgca aactcggcta cgtgaacctg gagagcgggg cgatgtaccg cgcactggcg
     2521 ttgcgcgcca tcgagtggga tgcctcgttt gacgacgagg cggcgctgct caagatggcg
     2581 caggattcgc gcattactct ggaccgcatc atcggcggca atcgcgttct gctcaacgga
     2641 aaggacattt ccgcgcgcat ccgggagcgc gatgtgaccg agggagcgtc gcgggtttcg
     2701 gtgcatccca aagtacgcga gtggatggtg gcgcggcagc gcgaaatggg cctgggcggc
     2761 ggcgtggtga tggaaggccg ggatattggc accaaggtct ttcccgatgc cgacttgaaa
     2821 atttttctcg acgccgaccc ggtgattcgc gagcagcggc ggctcgatca gcagcgggtc
     2881 aggggcgcgc cggcggagag catggctgcg gaactccggg agcgggaccg ccgcgatcgc
     2941 acgcgagccg catctccact ggtggccgcc gaagatgccg tcgtgctcga ctcgacccat
     3001 atgaccgagg acgaggtgct cgatcgaatc caggagttgg tggagcaacg gctggcggcc
     3061 cgtccagcgt agttcgaccg cggcgttagc tgcgcttgat aaatttcaac gaagccgaat
     3121 tcatgcaata acgcaggccg gtaggcctcg ggccgtcatc aaacacgtga ccgagatgcg
     3181 cgc
//
LOCUS       DASWZT010000134        15436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_5787, whole genome shotgun sequence.
ACCESSION   DASWZT010000134 DASWZT010000000
VERSION     DASWZT010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15436
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5787"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..1471)
                     /gene="gabD"
                     /locus_tag="VGE92_03635"
     CDS             complement(17..1471)
                     /gene="gabD"
                     /locus_tag="VGE92_03635"
                     /inference="COORDINATES: protein motif:HMM:NF008415.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY1312939.1"
                     /translation="MKLNDPLLLREQCFIDGGWLPADDGATLDVHNPATAQKLGRIPK
                     MGAAETRRAIAAAAAALPAWAAKTAKERAVVLRRWFDLMLVHLDDLAVLMTAEQGKPL
                     AESKAEIAYAAAFLEWFAEEGKRLYGDVIPGHQADKRILVLRQPVGVVAAITPWNFPA
                     AMITRKAGPALAAGCTMVCKPATQTPYSALALAELGHRAGLPKGVFNVITGSAAAIGG
                     EMTSNPTVRKLTFTGSTQIGKQLMAQCATTVKKVSLELGGNAPFIVFDDADLDAAVQG
                     AIASKYRNTGQTCVCANRLLVQAGVYDEFTQRLVQAAGKLRVGDGLAGVTDQGPLIDA
                     KAVAKIEEHIEDAVAKGATITLGGKRHALGGTFFEPTILTQVTPKMLVAREETFGPVA
                     PLFKFQTEAEAIAMANDTEFGLAAYIYTRDLARSWRVSEAIEYGIVGLNTGIISTEVA
                     PFGGVKESGMGREGSKYGILDYTEMQYVCVGGVS"
     gene            complement(1478..2137)
                     /locus_tag="VGE92_03640"
     CDS             complement(1478..2137)
                     /locus_tag="VGE92_03640"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY1312940.1"
                     /translation="MGLNVELTAADGHRLSGYRADPAGEPHGGIVVIQEIFGVTRHIR
                     AVVDQYAAAGFVTLAPALFDRVERNVDVPYSDAAQGFSYVKALKDEQVALDLQAAIER
                     VSGAGKVGVVGYCWGGLQAYLTAARTRVDAAVVYYGGGINQHTAQKPRAPIMFHFGEM
                     DTHIPMSAVEQIKAAVAQGIFFLYPAGHGFNCTDRASFEPVSAQLALDRSLDFFHQHL
                     G"
     gene            complement(2167..3453)
                     /locus_tag="VGE92_03645"
     CDS             complement(2167..3453)
                     /locus_tag="VGE92_03645"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY1312941.1"
                     /translation="MLVGANPRRVAIIGGVRIPFVRGNGAYAQVGNQEMLTAALRGVV
                     ERFGLQGQRLDDVAAGAVMKHSSQWNLTRESVLGSGLAAETPGLDLQRACGTSLEAAI
                     LLGNKIALGQIDCAIAGGVDTVSDPPVVYPKSFQQLLLRSYRGKSTGERIGPWFGLRL
                     KNLRPVLPGIAEPRTGLSMGESTELMVKTWGITRAEQDQLALESHQKAAAAYAAGFYK
                     DLVQEYLGVSQDNNIRSDTSLEKLSKLRPVFDSSAAGTLTAGNSTPMTDGASAVLLAS
                     EAWAKERNLPVLAYLSYGKVAAVDFIDKKEGLLMAPAYAVPRMLNDAHLTLQDFDYYE
                     IHEAFAGQVLCTLKAWADPVFCREKLKLPEALGSVDRSKLNVKGGSLAIGHPFAATGT
                     RIVATLAKILDESKAKRGLISVCTAGGMGVTAIVER"
     gene            3518..5197
                     /locus_tag="VGE92_03650"
     CDS             3518..5197
                     /locus_tag="VGE92_03650"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEY1312942.1"
                     /translation="MSRFSHAILHGIAFLGLSACGASSAPAPALPPVANPVAPHDDLS
                     RIVARYWDERAFPGKAQSPQFMADALAVERRYLAEVAAIPRASLDADARLNLDIFKRQ
                     RELDVEGFTYPIELIPIDPFNGPPLQLARAAADCGQFPFKTVKEYENWLLRIDDYVAW
                     TNQAIENMRAGLRRGYSSPRAVVERMLPLLQGFGEDGAANVFYVPWRGMPKDFPEAER
                     TRLAWRLNSAIKDKLLPAFRELHEFLQREYLPRARLTVALTVLPLGPPWYAYRVKRAT
                     GTALTPDEIHSIGLAEVDRGRTRLNSLNPGAPASAGELLSGYQDLKAQTLAALPTLFA
                     ALPRADFELRDAVPFGGSADPLVYQPAVAQRAVPAILYVNSAALPRVDSASFLQEAIP
                     GRHYQSALQQERADLPKFRQYGDEPAFVAGWALYAASLGDELGLYRDDEAKRDAVIGQ
                     LRCAAALVVDTGLHAKNWTRAQAIDYLRSKLAVNDADAGFMTDQWVASPGDALACKIG
                     ELKIQSLRNRAQQTLGAKFDLREFHSEILRGGAMPLDILEARIKLWMETRP"
     gene            5273..5851
                     /locus_tag="VGE92_03655"
     CDS             5273..5851
                     /locus_tag="VGE92_03655"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY1312943.1"
                     /translation="MRPSFYFAALLFTAPAAPAANLADSAQQGDIEVAVALDSGEQSG
                     SASASVRIQASRQVVWSLITSCGEELRLVPGLLVCEVMETAADRSWQQIRQVMDYSWY
                     MPKLSYVIHASYDPPSRVSIDRVSGDLLRLRGSWVLQSDGNFTIAHYAVDLEPGFWVP
                     HWIERAALKRDLPKMLRALRTRAEFVQQQKRE"
     gene            5859..6329
                     /locus_tag="VGE92_03660"
     CDS             5859..6329
                     /locus_tag="VGE92_03660"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY1312944.1"
                     /translation="MLTRMMSVWLALACGMAACDRTPEPPPPRVKQLFHLNPYEKDFG
                     YSQAVLIEKTLYISGSVAADQNGRLVAPGDMAGQMRAAYSNIRRTLAAHGAGFDEVVK
                     ETIFTTDMDAFLKAADLRFEFYDKERLPTTSWVQVQRLVDPGFLVEIEVVAELP"
     gene            6355..7926
                     /gene="gshA"
                     /locus_tag="VGE92_03665"
     CDS             6355..7926
                     /gene="gshA"
                     /locus_tag="VGE92_03665"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEY1312945.1"
                     /translation="MVDKVFERRVAGLVNSGERGVLRGGRKGVEKESLRVSPAGDIVQ
                     TPHPPALGAPLTNEHITTDFSESLIELVTPPFGETWELLQYLCDIHQFVYRHLDDELL
                     WATSMPCALEGDASIPLAQFGTSNVGRMKNIYRHGLGVRYGRIMQAISGVHFNYSFSP
                     RLWGVLGDVNQSKRPQQSFISEQYFGVLRNYRRYGWLVLYLFGTSPAVSKSFFAGREI
                     SLAKLDADTFYEPYGTSLRMSDIGYRNKNQATVNVSVNSLDEYVRDLSRAISTPFPPY
                     EKIGVKVNGVYQQLNANILQIENEYYSYIRPKRVARSGERPTKALQRAGVEYVEVRAL
                     DVSAFDPVGVNQNKLRFLEAFLALCLLRESAPIGQSEQAALDANHLVVARRGREPNLM
                     LKREGRDFPMLEWARELLDAMQGLCELLDHGEAARPYATALEQQRVKIDDVERTPSAR
                     LLTEMRQTGESFFQLAQRMSKLHKDYFLDLYPPNERRLAELAAAAGESHQRQAHIEAA
                     DKIDFDTYLAHYLAD"
     gene            8053..9285
                     /locus_tag="VGE92_03670"
     CDS             8053..9285
                     /locus_tag="VGE92_03670"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY1312946.1"
                     /translation="MSSPDRLPSADLPDSPQARQLHAGFPWLTFNPELEPEFRHAHFA
                     ENLLHTRVNLCLAVVITIAFSALESIVLGPELNRVPSMIHMLVIIPTLLVGLAASFAS
                     QRNRIYPPLALIASTILGLGVAAIQIIATLGGMSILFPCLMLTIIFIYFMGGLIFYHA
                     LAANMIVMFVYLAAGTALQLPGREFAYDALAMVAANLFGASVVYMHEKTSRMRFLEAC
                     LLREMVARDGLTGIQNRRMFDQHIQRVWQQAVREQERVAVLLADIDCFKDYNDRYGHQ
                     AGDECLRAVAMSLSQCARRPLDFVARYGGEEFAVVLYEASREYVAEVLTRIQRSIAEL
                     NIPHEASRVASRLTVSIGAAFIRPSSNRTPEGLIQLADEALYSAKEQGRNRVVVMEAE
                     YQSLRTGRFEKRRGQHAE"
     gene            complement(9295..10212)
                     /locus_tag="VGE92_03675"
     CDS             complement(9295..10212)
                     /locus_tag="VGE92_03675"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEY1312947.1"
                     /translation="MFGLAIHGGAGTLPRAEAGGEKERSYRAGLAAALDAGYSVLQQQ
                     GTSLEAVTRAVMVLEDDPLFNAGRGSVFTYDGLNELDAAIMDGSTLKAGAVCGVTHIR
                     NPIQLARTVMEHSEFVMLYGAGAEEFALSRGFALVPRSYFYTAERWRQLERIRGGDTG
                     LSALTISHVGTVGAVALDSGGRLAAATSTGGMTAKRYNRIGDSPVIGAGTYADDRSCA
                     VSATGHGEVFIRAAVAHDICARMRFGGRSLQAAVHEVVLEELPALHGEGGVVAIDRRG
                     EIAMEFNSEGMFRASRKQGEAAQMAIYRP"
     gene            complement(10259..10579)
                     /locus_tag="VGE92_03680"
     CDS             complement(10259..10579)
                     /locus_tag="VGE92_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY1312948.1"
                     /translation="MPKIVFIEPGGSRREIDAPLGITLMEVARQNGVQGVVAQCGGAC
                     ACATCHVYVDPAWLARLGPREEMEEAMLETAWEPRDNSRLSCQIHITADMDGLRVTVP
                     QRQG"
     gene            complement(10579..11793)
                     /locus_tag="VGE92_03685"
     CDS             complement(10579..11793)
                     /locus_tag="VGE92_03685"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1312949.1"
                     /translation="MISTIIIIGGGQAGAQAIDTLRREGYGGRLVLIGNEPELPYQRP
                     PLSKKYLSGEMAAERLVFRHRSFYDEHAIELKLGQAAVRLDPAARQVTLADGEKLAYD
                     RLLLCLGADSRRLTCPGATLPGVHYLRTVADVAPLREGFKPQTRIVVIGGGYIGLETA
                     ATGRKMGCEVTVLEMADRIMNRVVAPVVSQYFAGEHLLHGVKIVCDTRVVCLEGTGRV
                     ERVVCADGSTHAADLVVVGVGAVATTELANAAGLTCDNGIVVDEYCRTSDAAIFAAGD
                     CTNHPSLRFGRRVRLESVDNAFEQAKTAALNMLDRPHVHDRVPWFWSDQFDNKLLIVG
                     LSQDYDRQVLRGSPESRSFSVCYLKDRELLAVEAINHSKDYMAARKLIAERTPLQLDK
                     LADGGLGLKDAI"
     gene            11912..12250
                     /locus_tag="VGE92_03690"
     CDS             11912..12250
                     /locus_tag="VGE92_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY1312950.1"
                     /translation="MKLITAIIKPFKLDDVRAALSEIGVSGMTVTEVKGFGRQRGHTE
                     LYRGAEYVVDFVPKTRIEVAVKNELVDQVMEAIVGASKTGKVGDGKIFVTDILRVLRI
                     RTGETDNAAL"
     gene            12263..13942
                     /locus_tag="VGE92_03695"
     CDS             12263..13942
                     /locus_tag="VGE92_03695"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEY1312951.1"
                     /translation="MRLSDGCRLIAFTVLAASISEAGFAAKLTDLEHTRIEEPRPAGL
                     PSDAQLEAAGAVIGRIDIDTRNIFDESDARESNGLYQLANRLHIRSKRSSIKAQLLFA
                     SGDRYSARKLAETERTLRLLTYIYDARIVPVSYVDGTVDIKVITWDVWTLSPGISFGR
                     AGGSNTTNFNLQDSNFLGWGKALQVSHGSTVDRTSNTIAWTDPNVFGSRWTSMLMDVD
                     SSDGSQRALLVTRPFYSLDAAWSTKISAASFDRTISRYNLGEIVDQFNDNEASYELSG
                     GVSNGLIDGWTRRFTFGMRYDRNLFLPTASTATPARQLPPDRTLSYPFVGFDFLQDKY
                     AKVGDENQIGRTEDLYFGTEVSGEIGLSNGVFGADRSAIMLAAKALRGVQISELQQLF
                     LTSDFSSRVEDGRARNLIADAGAKYYWRWRPDWLLYAGLAGTVTSALDPDMQLLLGGD
                     NGLRGYPLRYESGTSRALFTVEQRVYTDWYPFRLVRVGGAIFADVGRTWGSGVIGNSD
                     PGLLRDVGFGLRLGNTRSGLGNVLHIDFAFPLNNIAGIQKFQFLVQTMQSF"
     gene            complement(13979..14416)
                     /locus_tag="VGE92_03700"
     CDS             complement(13979..14416)
                     /locus_tag="VGE92_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312952.1"
                     /translation="MAAAETAAAVTARIEARSADLLAVGLVHDDRMNIHLSRVIDNAP
                     VRDAALTVMLRGAAHPTVAEADGSYTLQTADLKVPGPAAVEFQVSEPSGTEALKGTLQ
                     LAGGAAQPEDKNSARQLGWWVLNFAVCIGFLWLYSRRRKAAKD"
     gene            complement(14494..15015)
                     /locus_tag="VGE92_03705"
     CDS             complement(14494..15015)
                     /locus_tag="VGE92_03705"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HEY1312953.1"
                     /translation="MTEIEVKPEHAADPNTRRVGILVGVVGILLSVVTIASHRAHTEA
                     VIHRTESNDAWAYYQAKKNREYTSDVALTLLHTLATEPAKSESAAQKLEAARSKYAAD
                     AEKIRDDATAKDAETKLEERRALRFDIGEGFLELGLVLCSLYFLARKSFFPIFGVLAG
                     VAGTAMGIWGCLL"
     gene            complement(15023..>15436)
                     /gene="ilvN"
                     /locus_tag="VGE92_03710"
     CDS             complement(15023..>15436)
                     /gene="ilvN"
                     /locus_tag="VGE92_03710"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEY1312954.1"
                     /translation="IESLSVAPTNDPTVSRVTLVTIGSDPVIAQINAQLLKLVDVVAV
                     EDMTSGDHLARELLLIKLQIAIEHVDAVAALLRRAGGRVLSSNPSSYIVELTSTETEI
                     GEFIANVAAFGDIVEVVRSGVLGIARANPRLHVIK"
BASE COUNT         2900 a         4888 c         4721 g         2927 t
ORIGIN      
        1 gccgagcaca ggtctgtcaa ctcactccac cgacgcacac atattgcatc tcggtgtagt
       61 cgaggatgcc gtacttcgag ccctcgcgcc ccattcctga ttccttgaca ccgccgaagg
      121 gcgccacttc ggtggaaatg atgcctgtgt tgagcccgac gatgccgtat tcgatggcct
      181 ccgagacccg ccagctgcgt gccagatcgc gcgtatatat gtacgcggcc aggccgaatt
      241 cagtgtcgtt ggccatggcg atggcctcgg cctcggtttg aaatttaaag agcggcgcca
      301 ccggaccgaa agtttcttcc cgggcgacca gcattttggg cgtcacctgg gtgaggatag
      361 tgggctcgaa aaacgttccg ccgagcgcat ggcgtttgcc gcccaacgtg atggtcgccc
      421 ctttggccac ggcatcctcg atgtgctcct cgattttggc gaccgccttg gcatcgatca
      481 gcggcccttg gtcggtgacg cccgccaagc catcgccgac gcgcagtttt ccggcggcct
      541 gcaccagccg ctgcgtaaac tcgtcgtaaa cgccggcctg caccagtaga cgattggcgc
      601 acacacaggt ttgaccggta tttcgatact tgctcgcgat cgctccctga acagcggcat
      661 ccaaatccgc atcgtcgaag acgatgaatg gcgcattgcc gcccaattcg agcgagacct
      721 tcttgaccgt cgtggcgcat tgggccatca gctgcttgcc gatctgggtg gatcccgtga
      781 aggtcagttt gcgtaccgtg ggattcgacg tcatttcgcc gccgatcgca gccgccgatc
      841 cggtgatgac gttgaatacg cccttgggca gcccggcgcg gtggccgagt tccgccaatg
      901 ccagcgcgga gtagggcgtc tgggtcgccg gcttgcacac catcgtgcat ccggccgcca
      961 gcgccggccc cgctttgcgc gtgatcatcg cggccggaaa attccatggg gtgatcgccg
     1021 cgaccacgcc gacgggttgg cgcagcacga gaattcgctt gtcggcttga tgacccggga
     1081 tgacatcgcc gtacagccgt ttgccttcct cggcaaacca ttcgaggaaa gcggcggcat
     1141 aggcgatctc agccttggac tcggccagcg gtttaccctg ctcggcggtc atgagtacgg
     1201 cgagatcatc gagatgcacg agcatgagat cgaaccagcg ccgcagcacc accgctcgct
     1261 ccttggcggt tttggcagcc cacgccggca gtgccgcggc ggcggcggca attgcacgcc
     1321 gcgtctcggc agcgcccatc ttcggaatcc gtccgagctt ttgagcggtg gccgggttat
     1381 gaacgtccaa ggtggcgccg tcgtcggcgg gtagccagcc gccgtcaatg aaacactgct
     1441 cgcgcagcag cagcggatcg ttaagtttca tgtgttctca gcccaagtgc tggtgaaaaa
     1501 aatccaaact gcgatcgagg gcgagttgcg cgctgaccgg ctcgaaactg gcccgatccg
     1561 tacagttgaa gccgtgacct gcgggataca gaaaaaaaat gccctgcgca acggcagcct
     1621 tgatctgctc gaccgcggac atcggaatgt gcgtgtccat ttcaccgaag tgaaacatga
     1681 tgggggcgcg cggcttttgc gccgtatgct gattgatgcc gccgccgtag taaacgaccg
     1741 cggcgtcgac acgggttcgg gcggcggtga ggtacgcctg cagcccgccc caacaataac
     1801 ccacgacgcc gactttgccg gcgccgctca cgcgctcgat ggcggcctgc aagtcgagcg
     1861 ccacttgctc gtctttgaga gccttgacgt aggagaaacc ctgcgccgcg tcgctatagg
     1921 gtacgtcgac gttgcgttcg acgcggtcga acagcgccgg cgcaagcgtc acaaatcccg
     1981 ccgccgcata ttgatcgacc accgcccgaa tatggcgagt caccccgaaa atctcttgga
     2041 tcacgacgat gccgccgtgc ggctcaccgg cagggtcagc gcggtatccg gacagccgat
     2101 gaccgtcggc cgcggtcagt tcaacgttca gtcccatgct taagaatacc gcgttcctcg
     2161 accccgtcaa cgctcgacga tggccgtgac ccccatgccg ccggcggtac aaaccgagat
     2221 caacccacgc ttcgccttgc tttcgtcgag aattttcgcg agcgtggcga cgatgcgcgt
     2281 gccggttgcg gcgaaaggat ggccgatcgc gaggctgccg cccttgacgt tgagtttcga
     2341 gcgatcgact gacccaagtg cctccggcag tttgagcttc tcgcggcaga acacaggatc
     2401 ggcccacgcc ttgagggtgc acagaacctg tcccgcgaag gcctcatgaa tttcgtagta
     2461 gtcgaaatcc tgcaaagtga ggtgcgcgtc gttgagcatg cggggcacgg cgtaggcagg
     2521 tgccatgagc aagccctctt tcttgtcgat gaaatccacg gcggccactt tgccgtagct
     2581 caagtaggcc agcaccggga ggttgcgctc cttcgcccag gcttcggacg ccaacaacac
     2641 cgccgaggcg ccgtcggtca tcggggtcga attcccggcg gtcaacgtac cggccgccga
     2701 cgaatcgaag accggcctga gtttcgacag cttctccaga ctcgtgtcgc tgcgaatgtt
     2761 gttatcttga gaaactccga gatactcctg taccagatcc ttgtaaaatc cggccgcgta
     2821 ggcggccgcg gccttctggt ggctctcgag cgccagctgg tcctgttcgg cacgggtgat
     2881 tccccaggtc ttcaccatga gttccgtgct ttcgcccatc gacaagccgg ttctcggttc
     2941 ggcgattccc ggcaggaccg ggcgcagatt cttgaggcgt aagccaaacc aggggccgat
     3001 gcgttcgccc gtcgacttgc cgcgataact tcgcaacagc agctgttgga aactcttggg
     3061 atagaccacc ggcggatcgc ttacggtgtc gactccgccg gcaatcgcgc agtcgatctg
     3121 gccgagggca atcttgttgc ccaacaggat cgccgcctcg aggctggtgc cgcaagcacg
     3181 ctgcaaatcg agccccggcg tttccgcggc gagccccgat ccaagcacgg actcgcgcgt
     3241 caagttccat tgactcgagt gcttcatcac cgcgccggcc gcgacatcat ccagccgctg
     3301 accctgaagt ccgaagcgct cgacgacgcc gcgcagcgcg gcagtcaaca tttcttgatt
     3361 gcccacctga gcataagcgc cgttgccgcg aacgaacgga atgcgcacgc cgccgatgat
     3421 cgcgacgcgc cgcggatttg ctccaaccaa cataaggctg actcccgaat gaataaggtt
     3481 cgaattatga accaaccgac cggcttataa tcgattgatg agccgatttt cgcatgcgat
     3541 tttgcacgga attgcgtttt tgggacttag cgcttgcggc gcctcgtccg cgccggctcc
     3601 ggcccttccc ccggttgcca accccgtcgc accccacgat gacttaagtc gcatcgtcgc
     3661 tcgctattgg gatgaacgcg cgttcccggg caaagcgcag tcgccgcaat tcatggcgga
     3721 tgccttggct gtcgagcggc gctatctcgc cgaggtcgcg gccattccgc gcgcctcgct
     3781 cgatgccgac gcgcgcttga acctagacat cttcaagcgg caacgcgaat tggacgtcga
     3841 gggattcact tatccgatcg agctgatccc gatcgatcct ttcaacggtc cgccgctgca
     3901 gttggcccga gccgccgcgg attgcgggca attccctttc aagacggtca aggaatatga
     3961 gaattggctg ctgcggatcg atgactatgt cgcgtggacg aatcaggcga tcgagaacat
     4021 gcgcgcggga ttgcggcgcg ggtacagctc gccgcgtgcg gtggtcgagc gcatgctgcc
     4081 gctgctgcaa ggattcggcg aggacggtgc cgccaacgtg ttctacgttc cctggcgggg
     4141 catgccgaag gatttcccgg aggcggagcg cacgcgtctc gcgtggcgct tgaacagcgc
     4201 gatcaaggac aagttgctgc ccgcatttcg tgaattgcat gaatttctgc agcgcgagta
     4261 tttaccgcgg gcgcggctca cggtcgcact gacggtgctg ccgctcgggc cgccttggta
     4321 cgcataccgg gtcaaacgcg cgaccggcac ggcgctcacg cccgacgaga ttcacagcat
     4381 cggcttggct gaagtcgatc gcggccggac tcgtctgaat tctttgaatc ccggggcgcc
     4441 ggccagcgcc ggcgaattgt tgagcggtta tcaggacctc aaagcgcaga cgctcgcggc
     4501 gttgccgacc ttgttcgccg cgctgccgcg cgcggacttc gagctgcgcg atgcagtgcc
     4561 gttcggcggg tcggccgatc ccctcgtgta tcaaccggcc gtggcgcagc gtgccgtccc
     4621 cgcgatcctc tatgtgaaca gcgcggctct gccgcgcgtg gacagcgcga gctttctgca
     4681 ggaggccatc cccggacgcc actaccaaag tgcgctccag caggaacgag ccgacctgcc
     4741 caagttccgg caatacggcg acgaacccgc cttcgtcgcg ggctgggctc tgtatgccgc
     4801 ctcgctcggc gacgaactcg gtctgtaccg cgacgatgaa gccaagcgcg acgccgtcat
     4861 cggccagctg aggtgcgccg ccgcgctggt cgtcgacacc ggcctgcacg ccaagaattg
     4921 gacccgggcg caggcgatcg actaccttcg ctcgaagctc gcggtcaatg acgccgacgc
     4981 cggtttcatg accgaccagt gggtagcctc cccgggggat gcgctggctt gcaagatcgg
     5041 cgaactcaag atccagtcgt tgcgcaaccg ggcacagcaa acactgggag cgaagttcga
     5101 tctccgcgaa ttccattccg agatcctgcg cggcggcgcc atgcctctgg acattctcga
     5161 ggccagaatc aaattgtgga tggaaacccg gccttgacgg aaattgaaga aaacagccgg
     5221 caggcggcga tttccatcga acagtgagct gtcgtcggag tcagactatc ctgtgcgccc
     5281 atccttctat ttcgccgctc tgctgtttac cgccccggcg gcgccggcag ccaacctggc
     5341 cgactccgct cagcagggcg acatcgaggt ggccgtagcg ctcgattccg gggaacaaag
     5401 cggcagcgcg agcgcctcgg tcagaattca agcaagccgc caagtggtgt ggtcgctgat
     5461 cacgagttgt ggcgaagagc tgcgcctggt ccccggcctg ttggtttgcg aggtcatgga
     5521 aacggccgcg gatcggtcgt ggcagcagat ccggcaagtg atggactact cttggtacat
     5581 gcccaagctg agctatgtga tccacgcgtc ctacgacccg ccgtcgcgtg tctcgatcga
     5641 tcgagtctcg ggagacttgc tcaggctgcg cggctcgtgg gtgctgcaaa gcgacggcaa
     5701 tttcacgatt gcacactatg cggtcgatct ggaaccggga ttctgggtgc cgcattggat
     5761 cgaacgcgcg gccctcaagc gcgatctgcc gaagatgttg cgtgccttgc gcacgcgtgc
     5821 cgaatttgta caacagcaaa aacgggagta ggagcagcat gttgaccaga atgatgagcg
     5881 tgtggctcgc gctggcttgc gggatggcgg cttgcgatcg tacgcccgag ccgccgccac
     5941 cgcgggtcaa gcaactgttc cacttgaacc cttacgaaaa ggatttcggc tattcccaag
     6001 ccgtattgat cgagaaaacg ctgtatattt ccggttcggt ggccgcagac cagaatggtc
     6061 gcctggttgc cccgggcgac atggcgggac agatgcgcgc agcgtactcc aatatacgcc
     6121 gcacgctggc cgcgcacggc gccggcttcg atgaagtcgt caaagagacg atatttacga
     6181 ccgatatgga tgcgttcctg aaagccgccg atttgcggtt cgagttttac gacaaagagc
     6241 ggctgccgac gacatcgtgg gtacaggtgc agcgactggt agatccggga tttctcgtgg
     6301 agatcgaggt cgtcgccgag ctgccataaa tcgaggatcg aggcattgca caacgtggtt
     6361 gacaaggttt tcgaacgccg cgtcgcggga ctcgtcaatt cgggcgaacg cggcgtattg
     6421 cgcggcggcc gcaagggcgt ggagaaggaa tcattgcgcg tctcccccgc cggcgacatc
     6481 gtgcagacgc cgcatccgcc ggctctcggc gcaccgctca ccaacgaaca tatcaccacg
     6541 gacttttccg agtcgctgat cgagctggtt acgccgccat tcggcgaaac ctgggagttg
     6601 ctgcagtatt tgtgcgatat ccatcaattc gtgtatcgcc acctcgacga cgaactattg
     6661 tgggcgacca gcatgccgtg cgctctcgag ggcgacgcca gcatcccgct cgcgcagttc
     6721 ggaacttcga atgtcggccg tatgaagaac atttaccggc acgggttggg ggtgcgctac
     6781 ggccgcatca tgcaggcaat ttcaggcgtg cacttcaact attctttttc gccgcggcta
     6841 tggggcgtgc tcggcgacgt gaatcaatcc aagcggccgc agcagagctt catatccgag
     6901 caatacttcg gcgtgttgcg caactaccgg cgctacggct ggctggtgct gtacttgttc
     6961 ggcacctcgc cggcagtgtc gaagagcttt ttcgcaggcc gcgagatcag cctggcaaag
     7021 ctcgacgccg acacgttcta cgaaccgtac ggaacgtcgc tacgcatgag cgacatcggc
     7081 tatcgcaaca agaatcaagc gacggtcaat gtttcggtca acagcctgga cgagtatgtg
     7141 cgcgatctat cgcgcgccat aagcacaccg ttcccgccgt acgagaagat cggcgtcaag
     7201 gtcaacggcg tctatcagca gttgaacgcc aacatcctgc aaatcgaaaa cgaatactac
     7261 agctacatcc ggccgaagcg ggtggcgcgc tccggcgagc gtccgaccaa ggctttgcag
     7321 cgcgccggcg tggaatacgt cgaagttcgc gcgctggatg tgagcgcgtt cgacccggtc
     7381 ggcgtcaatc agaacaagct gcgttttttg gaagccttcc ttgccttgtg tctgctgcgg
     7441 gagagcgcgc cgataggcca gagcgagcaa gctgccctcg atgccaacca tttggtggtt
     7501 gcgcggcgcg gccgtgaacc gaacctcatg ttgaaacgcg aggggcgcga ctttcccatg
     7561 ttggagtggg cccgagaatt gttggacgcc atgcaggggc tgtgcgaact gctggatcac
     7621 ggcgaggctg cgcgccccta tgccacggct ctcgaacaac aacgcgtcaa gatcgacgac
     7681 gtcgaacgaa ccccgtccgc gcgcctactc acggagatgc gccagaccgg ggagtcgttt
     7741 tttcaactgg cgcagcgcat gtcgaagctg cataaggact actttctgga tctttatccc
     7801 cccaacgagc gccgtctggc cgaattggcc gccgccgccg gcgagtcgca ccagcgccaa
     7861 gcgcatatcg aggcggccga caagatcgat ttcgacacct atctcgcgca ttacctcgcc
     7921 gattgacccg ccactgcgac cagggagcgg tcttatttaa cataatcaag cgcaactctc
     7981 gcggggtttt ttactccaaa gggtggcgta ggtcattgag tacacaagct cgggttgtgt
     8041 acggtgaggc gaatgagcag ccccgatcga cttcccagcg cagatctgcc ggattcgccg
     8101 caggcccggc aactgcacgc gggctttccc tggctcacgt tcaatcccga actcgaacct
     8161 gaattcaggc acgcgcattt cgccgagaac ttgctgcata cgcgtgtgaa tctgtgcctg
     8221 gcggtggtga tcacgatcgc cttttccgcg ctggaatcga tcgtgctcgg tcctgaactc
     8281 aatcgcgttc cgagcatgat ccacatgctg gtgatcattc ccacgctgct cgtgggactg
     8341 gcggcgagct tcgcatcgca gcgcaatcgg atctatccgc cgctcgcgct gatcgcttcg
     8401 accatactcg gcctcggcgt ggcggcgatt cagataatcg ccacgctcgg cggcatgtcg
     8461 atcttgtttc cttgcttgat gctgacgata attttcatct atttcatggg cggcttgatt
     8521 ttctatcacg cgctcgcggc aaacatgatc gtgatgttcg tctaccttgc ggccggcacc
     8581 gcgttgcagc tgccgggacg cgagttcgcg tacgacgcgc tggcgatggt agccgccaat
     8641 ctattcggcg cctcggtggt ctacatgcac gaaaaaacca gccgcatgcg ctttctcgag
     8701 gcgtgcctgc ttcgagaaat ggtggcgcga gacggcctga ccggcatcca aaaccgccgc
     8761 atgttcgacc agcacatcca gcgcgtctgg cagcaggcgg tgcgcgagca ggagcgcgtt
     8821 gccgtgttgc ttgccgacat agactgtttc aaggattaca acgatcgtta cggacatcag
     8881 gccggcgacg aatgcctgcg cgccgtggcg atgtccctga gtcaatgcgc acgccgaccc
     8941 ttggatttcg ttgcgcgtta cggcggcgag gaattcgcgg tggttctgta cgaagcgagc
     9001 cgcgagtacg tcgcggaagt gctgacgaga attcagcgct cgatagcgga gctcaatatc
     9061 ccgcacgaag cgtcgcgggt ggccagtcga ctgacggtga gcatcggcgc ggcattcatc
     9121 cgcccgagca gcaatcgcac gcccgaaggg ctgattcagc tcgcggacga agcgctgtac
     9181 agcgccaaag agcagggtag gaaccgcgtg gtcgtgatgg aagccgaata ccagagtcta
     9241 cgcaccggcc gtttcgagaa gcgccgcggc cagcacgccg agtagcggcg caggctacgg
     9301 ccgatagatc gccatctgcg cggcctcgcc ttgtttgcgg ctggcgcgaa acatcccttc
     9361 cgaattgaat tccatggcga tctctccgcg gcggtcgatc gcgacgacgc cgccctcgcc
     9421 gtgcaacgcc ggcagctcct cgagcacgac ctcgtgtact gccgcctgca ggctgcgccc
     9481 gccgaagcgc atgcgcgcgc agatatcgtg cgccaccgcg gcacgtatga agacttctcc
     9541 atgccccgtg gccgacaccg cgcacgagcg atcgtccgcg taggtacccg cgccgatgac
     9601 gggtgagtcg ccgatgcggt tataacgctt ggccgtcatg ccgccggtgg acgtggccgc
     9661 ggccagccgg ccgcccgagt ccaacgcgac cgctcccacg gtgccgacgt gcgatatcgt
     9721 cagcgccgat aatccggtat caccgccgcg aatgcgttcg agctgccgcc agcgttcggc
     9781 ggtgtagaaa tagctgcgcg gcaccagcgc aaaaccccga ctcaaggcaa actcctccgc
     9841 tcccgcgcca tacagcatga cgaattcgga atgctccatg acggtgcgcg ccaactggat
     9901 tggattcctg atatgggtca caccgcatac ggcgcctgcc ttgagcgtgc tgccgtccat
     9961 gatcgcggcg tccaattcat ttagtccgtc gtacgtgaac accgagccgc gcccggcgtt
    10021 gaacaacgga tcgtcctcga gcaccatcac ggcgcgcgtg accgcctcga gactcgtgcc
    10081 ttgctgctgc agcaccgagt aacccgcatc gagtgccgcg gccaatccgg cacggtagct
    10141 gcgttccttt tcaccacctg cttcagcgcg cggcaaagta ccagcgccgc catgaatcgc
    10201 taagccgaac atctggatga gttctcctgg tcgctcaaac gccgcaaggc tatcacggct
    10261 atccctgccg ctgcggcacc gtcacccgca gtccgtccat gtccgcggtg atgtgaatct
    10321 ggcaactcag gcgactgttg tcgcgcggct cccaggcggt ctcgagcatg gcttcctcca
    10381 tttcttcgcg cggccccaat ctcgcgagcc atgccggatc gacgtacacg tgacaggttg
    10441 cgcacgcgca ggcgccgccg cactgcgcca ccacgccttg cacgccgttc tggcgggcga
    10501 cttccatcaa cgtgatcccg agcggcgcgt cgatttcccg ccggctgccg ccgggctcga
    10561 tgaacacaat cttcggcatc agatcgcgtc tttgaggccg agcccgccgt ctgccagttt
    10621 gtccaattgc agtggcgtac gctccgcgat cagcttgcgc gcggccatgt agtccttgga
    10681 atggttgatc gcttccaccg cgagcaattc ccggtctttg aggtagcaga cgctgaaact
    10741 tcgcgactcg ggactgccgc gcaacacctg gcggtcgtag tcctgcgaaa gcccgacgat
    10801 gagcaacttg ttgtcaaact gatcggacca gaaccacggc acccggtcgt gcacgtgagg
    10861 ccgatccagc atgttgagtg cagcggtctt ggcttgttcg aaggcattgt ccacggactc
    10921 gagccgtacc cggcggccga agcgaagcga cggatggttg gtgcaatcgc ccgcagcgaa
    10981 gatggcggca tcgctggtgc gacagtattc gtcgaccacg atgccattat cacaggtcaa
    11041 cccggctgcg ttcgcgagtt cggtcgtcgc gacggcaccc acaccgacca cgacaaggtc
    11101 cgccgcatga gtgctgccgt cggcgcaaac cacccgttcg accctgcccg taccttcgag
    11161 acagacaacg cgggtgtcgc aaacgatttt gacgccatgc aatagatgtt ccccggcaaa
    11221 atattgcgag acaacgggcg caaccacccg gttcatgatc cgatcggcca tctcgagcac
    11281 ggtgacttcg cagcccatct tgcggcccgt cgccgccgtc tccagtccaa tgtagccacc
    11341 accgataacg acgattcgcg tctgaggctt gaaaccttcg cgcaggggag cgacatccgc
    11401 cacggtacgc aaataatgaa ccccggggag cgtcgcgccc gggcaggtca agcggcgcga
    11461 atcggcgccg agacacaaca ataaccggtc gtaggcgagc ttctcgccgt ccgccagtgt
    11521 gacctgccgc gcggcggggt cgagacgcac cgccgcctga cccagtttga gttcgattgc
    11581 atgttcgtca tagaaggagc gatgtcggaa cacgaggcgc tcggcggcca tttcgccgga
    11641 caggtatttc ttcgacagcg ggggtcgctg gtagggcagc tccggttcat tgccgatgag
    11701 caccagccgg ccgccatagc cttctcggcg cagggtatcg atcgcctgcg cgcccgcctg
    11761 gccgccgcct atgatgatga tggtggagat catggtctcc cttatagcgg gttgagctca
    11821 gggccgaaag ggcgatcggg acgcagatgc tcgtgccacc ggcttgcaat cgtaatagta
    11881 ttgtcgcggc ggccctcgag cggagtctag aatgaaattg attactgcaa tcatcaaacc
    11941 cttcaagctg gacgacgttc gggcggcact ctccgagatc ggcgtttcgg gcatgacggt
    12001 gaccgaggtg aagggtttcg gacgccagcg cggccacacc gaactgtacc gcggcgccga
    12061 atatgtcgtc gatttcgtgc cgaagacccg catcgaagtc gcggtcaaga acgagctcgt
    12121 cgatcaggtg atggaagcca tcgtcggcgc ctctaaaacc gggaaggtgg gcgacggcaa
    12181 gatcttcgtc accgatatcc tgcgggttct gcgtattcgc accggcgaga ccgacaacgc
    12241 cgcgttgtag ccgcgccgtc ggatgaggtt atccgacggt tgccggctga ttgcgttcac
    12301 cgtactcgcc gcgtcgatct ccgaagcggg tttcgcggcc aaactcaccg atcttgagca
    12361 cacgcgcatc gaggaaccgc gccccgcggg gctaccgagc gatgcgcagc tggaagcggc
    12421 cggagccgtc atcggccgga tcgacatcga tactcgaaat atcttcgacg aaagcgatgc
    12481 gcgcgagagc aacggactgt atcaactcgc aaaccgtctg cacatacgca gcaaacgctc
    12541 gagcatcaaa gcacaattgc tgtttgccag cggcgatcgt tacagcgccc gcaagctcgc
    12601 ggagaccgaa cgaacgttgc gcttgctgac gtacatctac gacgcgcgca tcgtgccggt
    12661 gagctatgtc gacggcacgg tcgatatcaa agtgatcacc tgggacgttt ggaccttgag
    12721 tccgggcatc tccttcggac gcgcgggcgg cagcaacacc acgaatttca accttcaaga
    12781 ctcgaacttc ttgggctggg gaaaggcgct gcaggtgtcg cacggcagca ccgtcgaccg
    12841 caccagcaac accatcgcgt ggaccgaccc gaacgtgttc ggctcgcgct ggacgtcgat
    12901 gctcatggac gtcgactcga gcgacggttc gcagcgtgcg cttctggtca cgcgtccgtt
    12961 ctattcgctg gatgcggcgt ggagcacgaa aatctcggca gcgagtttcg atcgcaccat
    13021 ctcccgctac aacttggggg aaatcgtcga tcaattcaat gacaacgagg cctcttacga
    13081 attgagcggc ggcgtctcca acggcttgat cgatggctgg acccggcgct ttaccttcgg
    13141 catgcgctac gaccgcaatt tgttcctgcc gacagcgtcg accgcgacac ccgcacgcca
    13201 gctgccgccg gaccgaacgc tgtcctatcc tttcgtcggc ttcgactttc tacaggacaa
    13261 gtatgcaaag gtcggtgacg aaaaccaaat cggccgcacc gaggatttgt acttcggcac
    13321 cgaagtcagc ggcgagatcg gcctgtcgaa cggcgtattc ggcgcggatc gcagcgccat
    13381 catgctggcc gcgaaggcgc tgcgcggagt gcagatcagc gagctgcagc aactgttcct
    13441 gaccagtgat ttctcatccc gcgtcgagga cggccgtgcc cgaaacctga tcgccgacgc
    13501 cggcgccaaa tactattggc gttggcgccc ggattggctg ctgtacgcgg gtcttgcggg
    13561 aacggtgacc agcgcgctcg atcccgacat gcaactcttg ctcggcggcg acaacggcct
    13621 gcgaggctat cctctgcgct acgagtcggg cacctcgcgg gcgctgttca ccgtcgagca
    13681 gcgcgtctat accgattggt acccgtttcg cctggtccgg gtcggtggcg ccatcttcgc
    13741 cgacgtgggc cgtacttggg gcagcggggt catcggcaac agcgatcctg gactgctgcg
    13801 cgacgtcgga tttgggctgc ggctcggcaa tacgcgctcc ggcctgggca acgtcctgca
    13861 catcgatttc gcctttcctc tcaacaacat cgcgggaatc cagaaattcc aattcctggt
    13921 gcaaacgatg cagagtttct aggcgcacgg cgagtggcgc acggcgagcg gcggaaggct
    13981 aatcttttgc tgctttgcgg cgccgcgaat agagccacag gaaaccgatg cacaccgcga
    14041 aattgagcac ccaccacccg agctggcgcg ccgaattctt gtcctccggc tgcgccgcgc
    14101 cgccggcgag ctgcaaggtt cccttgagcg cctcggtccc ggaaggctca gacacctgaa
    14161 actcgaccgc cgcgggaccg ggaaccttca gatctgcagt ttgcagcgta tagctgccgt
    14221 ccgcttcggc gaccgtgggg tgtgccgctc cgcgcagcat caccgtcaat gccgcgtctc
    14281 gtaccggcgc attgtcgatc acgcggctca agtgaatgtt cattcgatcg tcgtgcacca
    14341 ggcccaccgc gagcagatcg gcggagcgtg cttcgatccg cgccgttacc gcggcggccg
    14401 tttcggcggc ggccatgcaa gccgtcgcga gcgccgacgc ggcgatgccc aacgcggcgc
    14461 agcgaaggca acggctccgc gccgacccct tgctcaaagc aggcagcccc aaatgcccat
    14521 cgcggtgccg gccacgccgg ccagcacgcc gaagatcggg aagaagcttt tgcgcgccag
    14581 gaaatacagg gagcacagca cgagaccgag ttccaagaac ccctcgccga tgtcgaagcg
    14641 caacgcccga cgctcctcga gcttggtttc agcgtctttg gcggtggcgt cgtcgcggat
    14701 tttctcagcg tccgcggcgt atttactgcg cgccgcctcg agcttttgcg cggcactctc
    14761 cgacttggcc ggctccgtcg ccagggtgtg cagcaaggtg agcgccacat cgctggtgta
    14821 ctcgcgattc ttcttcgcct ggtagtacgc ccatgcatcg ttcgattcgg ttctatgaat
    14881 caccgcctcg gtatgcgcgc gatgcgacgc gatcgtgacc accgacagca gaatcccgac
    14941 cacgcccacg agaattccga cccggcgggt attgggatcg gcagcatgtt ccggcttgac
    15001 ttcaatctcg gtcatggcgc aatcatttga tgacgtgcag gcgcgggttg gcgcgcgcga
    15061 taccgaggac gccgctgcgc accacttcga cgatgtcgcc gaatgcggcg acattggcga
    15121 tgaattctcc gatctcggtc tcggtgctcg tgagttcgac gatataactc gacggattgg
    15181 acgacagcac gcgtccgccc gcgcggcgca acagcgcagc cacggcatcg acgtgctcga
    15241 tggcgatttg cagtttgatc agcagcaatt cgcgggccag atgatcgccg ctggtcatat
    15301 cctcgaccgc caccacatcg acgagcttca aaagctgagc gttgatctgc gcgatgaccg
    15361 gatcggagcc gatcgtgacg agggtgacgc gcgacacggt cgggtcattg gtcggtgcca
    15421 ccgacaagga ctcgat
//
LOCUS       DASWZT010000135         1762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_73576, whole genome shotgun sequence.
ACCESSION   DASWZT010000135 DASWZT010000000
VERSION     DASWZT010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73576"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..904
                     /locus_tag="VGE92_03715"
     CDS             275..904
                     /locus_tag="VGE92_03715"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY1312955.1"
                     /translation="MSEEIVFYHNPMSRGRMVHWMLEEVGVPYRIELLSFEKGEHKKP
                     AFLAVNPMGKIPAIVHRGAVVTECGAIITYLADAFPASKLAPTIEDPARATYLRWMFF
                     GQGCIEPALIDRMLSRPPADKPGAVGYGNYDDMLHTTEKALTPGPYILGDRFSAVDVY
                     IGSQLGFGMMTKSLAPQPLFQSYVDALFERPAYKRAIEQGEKLAARLKN"
     gene            complement(901..>1762)
                     /locus_tag="VGE92_03720"
     CDS             complement(901..>1762)
                     /locus_tag="VGE92_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY1312956.1"
                     /translation="IGAVAFSGKAIIVKFAYRYGVDAVTLLMYRMLFALPMFLTLTWW
                     SGRGKPGLTGRDARLVLFLGFCGYYLASYLDFAGLFYISASLERLILYLNPTLVLGLS
                     VLLFKRKVSGGQIVALALSYAGVLCVFGREVTLAGANVPLGTSLVFGSTVSYAVYLAY
                     SGEEVRRLGALRLTGLATSVACIICIAQFLLLRPLSAMVVAPQVLWLTLLNATVCTFA
                     PVVMVMMAVERIGASLTAQAGTIGPLSTILMAVLLLGEPFTVWVAAGTLLVVVGIWLL
                     ARQPPPKAAL"
BASE COUNT          388 a          537 c          548 g          289 t
ORIGIN      
        1 atggcaaaac ccccatcaag gtccgctggt atagcgacag ctacgtcgaa atcccggttc
       61 gcagatagcg cggcgttgcc cgcgggttga gttcgagcac tgcgccaagg cgcggtccca
      121 ggcggtcgag cgtcgcgatc ctatatatcg cccaggctca actcctcgaa ggcggcggaa
      181 ggcttggcgc caaatgagat ctcaggggca gcgagtccct ggccggttga tttagaatca
      241 accgagcgct gtcacaacca agggggaagc ctgcatgagc gaggaaatcg tgttttatca
      301 caatccaatg tcgcgcggcc gcatggtgca ctggatgttg gaagaggtcg gcgttccgta
      361 tcggatcgaa ttgctgagtt tcgagaaggg cgagcacaag aagcccgcgt ttctggcggt
      421 taaccccatg ggaaagattc cggcgatcgt gcatcgcgga gcggtagtca ccgaatgcgg
      481 agcgatcatc acctatcttg ccgatgcgtt ccccgcgtcc aagctggccc ccacgatcga
      541 ggatcccgcg cgcgcgacgt acctgcggtg gatgtttttc ggccagggct gtatcgagcc
      601 tgcgttgatc gatcgcatgc tgtcgcggcc gcccgccgac aaacccggcg cagtcggcta
      661 cggcaactac gacgatatgt tgcacaccac ggagaaggcc ctgacgcccg ggccctacat
      721 cttgggtgat cgattcagtg ccgtcgatgt gtacatcgga tcgcagctgg ggttcggaat
      781 gatgaccaag agtttggcac cgcaaccgct gtttcaaagc tacgtggatg cgctattcga
      841 gcgtccggcg tacaaacgcg ccatcgagca gggcgaaaag ctggcggcgc gattgaagaa
      901 ttaaagagcg gcctttggcg gcggctgcct ggcgagcagc caaatgccca ccacgaccag
      961 caaggtgcct gccgccaccc agaccgtgaa cggctcgccg agcaacagca ccgccatgag
     1021 gatggtcgac aaggggccga tcgtgccggc ctgggcggtc agcgacgccc cgatgcgctc
     1081 gacggccatc attaccatga cgacgggtgc gaaggtgcat acggttgcgt tgagcagcgt
     1141 cagccacaga acttgcgggg ccaccaccat cgcgctcaac ggccgcagca acaagaattg
     1201 ggcaatgcag atgatgcacg ccacgctcgt cgcgaggccc gtgagacgca gagcgccaag
     1261 gcgtctgact tcctcgccgc tgtaggccag gtagaccgca tagctcaccg tgctgccgaa
     1321 cacgagactc gtaccaagcg gcacgtttgc gcccgccagc gtgacttccc tgccgaacac
     1381 gcacagaacg ccggcatagc tgagggcgag cgcaacgatc tggccgccgc tcaccttgcg
     1441 cttgaacagg aggacactca accccaacac cagggtcgga ttcaaataga gaatgagccg
     1501 ttcgaggctg gcgctgatgt agaacagtcc cgcgaaatcg agataactcg ccaggtagta
     1561 accgcagaag ccgaggaaca ggaccagccg cgcgtcgcgc ccggtaagcc cgggtttgcc
     1621 ccgtcccgac caccacgtga gcgtcaggaa catcggcagg gcgaacaaca tccgatacat
     1681 cagcaatgtg acggcatcga ctccataacg gtaggcgaac ttgacgataa tggctttgcc
     1741 ggagaaggcg accgccccga tg
//
LOCUS       DASWZT010000136         7533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_92984, whole genome shotgun sequence.
ACCESSION   DASWZT010000136 DASWZT010000000
VERSION     DASWZT010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7533
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92984"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VGE92_03725"
     CDS             <1..196
                     /locus_tag="VGE92_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312957.1"
                     /translation="QHHRLIRGAAVVAAPVLVVSLAVLAAGALEPAVVALLTGVDAVC
                     VGTRFELLIPLTGVGRTGPP"
     gene            266..439
                     /locus_tag="VGE92_03730"
     CDS             266..439
                     /locus_tag="VGE92_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312958.1"
                     /translation="MLEVALLVAAVVAALGAAGAVLAAVVLGAVAALTAAVLLAAVVP
                     AAVDEDDFDAVTA"
     gene            886..1332
                     /locus_tag="VGE92_03735"
     CDS             886..1332
                     /locus_tag="VGE92_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312959.1"
                     /translation="MSLITNLSSASSAQPATNIRPHGHKHGFHVDPTTDSSTDTAAQV
                     PVGTAQNLFGSLLRSLEQAIGVQPSTATPAATATSSTPTTATTAASIASAATAAASTS
                     AAPSANPTATQSASALLQNYLHNAAQNLKADGSTLAGIAGNLRINV"
     gene            1400..2206
                     /locus_tag="VGE92_03740"
     CDS             1400..2206
                     /locus_tag="VGE92_03740"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEY1312960.1"
                     /translation="MNAIKRLPTLFIPHGGGPCFFIEPPPTNPHMWDGLAAYLRGIDA
                     SIGVRPKAVLVISGHWENPLPTLNIAAKPGLLFDYYGFPDHTYRLKYPAAGAPELAER
                     VRKLLDGAGFQSDVESKRGLDHGVFVPFLLIYPNADVPILQLSLQASLDPGLHLELGK
                     ALAPLRDQGILIVGSGMSYHNLADMFSGRGAEAAKTFDVWLGDAVQDPATRNELLIKW
                     RDAPGGAASHPREEHLIPLMVAAGAAQGERGVRSFGESIGGKQISGFQFG"
     gene            2199..3524
                     /locus_tag="VGE92_03745"
     CDS             2199..3524
                     /locus_tag="VGE92_03745"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY1312961.1"
                     /translation="MAKNPAALLACGLLIAACPAVFGADAPTLVIRAHSLIDGTADKP
                     RQNMDVVIRGNRIVEVHPTNSQALPAAVQVIDLGAATLLPGLIDTHTHIFLQGEDPAA
                     GGYDVQLLKFPASYRAARAVVAARRALEQGFTSIRDMETEGAGYGDVGIKRAINEGII
                     PGPRMWVSTLSISTTGGYPLEGYAPEIAALPKGSQLIDGPIEARKAAREQLDNGADWI
                     KVYMTHRSWLDAQGALVSQPTLTVEELKAIVDETHGWGKKVACHAYNGIGLQRALDGG
                     CDSIEHGLEITDAQIAQMLKQGTWYVPTLQVYYTDWDPADTDAGKRDRKRAELHGVSF
                     GKALRAGVKIAFGTDVGGFKWSEPIAEEFSREVQLGMTPMQAIQSATSRAAELLGKRG
                     ELGVIAPGAYADLIAVASDPLADVAVLKAVQFVMKDGVVFKSPRGSVAP"
     gene            complement(3527..4894)
                     /locus_tag="VGE92_03750"
     CDS             complement(3527..4894)
                     /locus_tag="VGE92_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY1312962.1"
                     /translation="MSDSSSGGRAKRYSLIGIAAILGVMLLGFLWFVVLAPHPMDFAG
                     GSPVRLADYRAQDPTGVPPELKDASLIQKGEYLTRAADCAACHTAEGGAPYAGGRAFV
                     LPFGTLYSTNITPDAETGIGRYSDANFLDAIHKGLGRNNTKLYPAMPFASYTYMTDAD
                     ALAIKAYLFSLAPVHAAAPENTLSFPFNQRSLMGIWALLFNPDKRFEPRTGRDPDWNR
                     GAYLVEAMEHCGECHTPRNLMQALDNRKKFSGAVQAGWRAYNITADQKSGIGTWDEAD
                     LAHYLAMGHADGRGTATGPMGEAVDNSLQYLTQSDIGAMVRYLRTVTAVATSDLPEPK
                     ANPAPSSYSQGAAAALDSRGRAVYAGACAGCHDWSGVSPVLPYATLTGVRSVNDPTAT
                     NVVQIILTGAHRHTADAGPAMPAFGSAYSDAEIASLANYVTARFGAQPSAVTAENIAT
                     LRAED"
     gene            complement(4942..6537)
                     /gene="lnt"
                     /locus_tag="VGE92_03755"
     CDS             complement(4942..6537)
                     /gene="lnt"
                     /locus_tag="VGE92_03755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEY1312963.1"
                     /translation="MLKLNFYGAFGLVTASGCLWFLAVTPFDYSALAWIAAVPMLFAI
                     DRTANFRRALLLGWWAGIVETAGGFYWLIDVMQRFADFPWWAAVLVFFLFCAARALIF
                     LLFTAVVCAIRAKRRVPMTLLAPVGMVSCELVIPQLFPCGQWISQAWHPLVIQIAELT
                     GPLGVTALLMLVNGALYDLAVDGRAARIPAAAAAALLAAALIFGAVRMQQVDARVARA
                     PTLHVGLVQPNFAYSVTGEFSRDEILRQLSALQSQTRRLQAAGADVVVWSEGAYPITL
                     PRDFAVDFAPNSLAMIRRGFSVPVVIGADMFDQAHEDAFNSAILLDGDGRVAGYYDKV
                     RLLAFGEYIPGIETFPWLRKFLPEGTGRFTAGRGPAVLTLRQPDGQGWRLGPVICYED
                     ILQGFLRGVGQLHPNLLVNLTSDSWFGADSEPWEHLALAVFATVELRVSMVRAVNSGV
                     SALIDPNGRVLQTTYADDPYRHPHAEDGVVVTAPGMPGGRTIYVLVGNLFAYLCLAAT
                     LALLAMAARARFRSIGRFRARDL"
     gene            6601..>7533
                     /locus_tag="VGE92_03760"
     CDS             6601..>7533
                     /locus_tag="VGE92_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY1312964.1"
                     /translation="MQERYEPAAVEADAQNFWNRQRCFEATEDQTREKYYCLSMFPYP
                     SGRLHMGHVRNYMIGDVLARFMRMNGKNVLQPMGWDAFGLPAENAAIANRVPPAKWTS
                     QNIANMKSQLKSLGFALDWSRELTTCHPEYYRWNQWLFLRMLEKGIAYQKTGVVNWDP
                     VDKTVLANEQVIDGRGWRTGALVEKREIPMYYLKITAYAEDLLEALDHMPGWPERVRT
                     MQANWIGKSVGVRLGFPYDIEGLGAGVMHAFTTRADTLMGVTFCAVAPEHPLAAFAAR
                     SHGKLAAFIEECQRGPAIESQLATQEKKGMPTGLS"
BASE COUNT         1454 a         2312 c         2462 g         1305 t
ORIGIN      
        1 tcaacaccac cgcctgatca gaggcgcggc ggtggttgcg gcgcccgtgc tggtggtgtc
       61 gctggcggtg ctcgccgccg gtgcgctcga accggccgtc gtcgcgctgc tcaccggggt
      121 cgatgcggtt tgcgtcggta ctcggttcga gttgttgatc ccattgacgg gcgttggcag
      181 gaccgggccg ccgtgaaccg ggcggctgac atcgcgaaac gcctgaaaga ggtcatagag
      241 gaaggattga agatcctgca ccaagttgct cgaggtggcg ctgctggtcg ccgcagtcgt
      301 ggccgcgctg ggtgcggcgg gggcggtgct cgccgcggtg gtgctgggcg cagttgccgc
      361 gctgacggcc gcagtgctgc tggcggctgt tgtgccggcc gcggtcgatg aggatgactt
      421 cgatgcggtc acggcttgag tgagcgcctg caacaaactc gcgaaaacgg tgagggcttg
      481 ctccgcgccg tcgttggacg agccgccgcc gccacggccg tgccgatgcg gttgctgggc
      541 cgaggcgact gtggccgcga ggggggccgt agacgcagcg gccgaaattg cattagttga
      601 cataatattg cccgtttggt gtttaccgtt ggacctatag cggcaccgga ccgcggctct
      661 tgagtgattt ctgaagtctg ttggcacaaa acctgcttcg tagtcagagc agggcagcag
      721 cagttgttgt cccgtgacaa gggatcagtc agccaacggg ccgaacgatg cggtacttac
      781 tcgatcgccg cgagattgcg gcaacacatg gccatccaag cggcggcaag cggcaattta
      841 ttgccgctcg atccctcatc aaccgattga agaaaggaaa tgcatatgtc gttaatcacg
      901 aacttgagct cagcgtcctc ggcgcaacct gcaaccaata tccgtccgca cggccataag
      961 cacggctttc acgtggatcc gaccaccgat tccagcaccg acaccgcggc gcaggttccg
     1021 gtggggacgg cgcagaatct attcggcagc ctattgcgat cactggagca ggcgatcggc
     1081 gtgcagccaa gcaccgcgac cccggccgcc actgcgactt cgagcacccc gacgacggca
     1141 accaccgcgg cgagcatcgc atcggccgcg acggccgccg cgagcacgtc ggcggctcct
     1201 tcggcaaatc cgacggctac acagtctgcg agcgctttgt tgcagaatta tttgcacaac
     1261 gctgcgcaga acctcaaggc cgatggatcg acgctggccg gaattgcggg gaacttgcgc
     1321 atcaacgtgt aaccgcaaat cgatcaattg acgttcacag ggcgcccggc taggctcaag
     1381 tgcgccggta aaatccctgg tgaacgctat taaacgactg ccaactttgt ttattcctca
     1441 tggcggcggg ccctgttttt tcatagagcc gccgccgacg aacccgcaca tgtgggacgg
     1501 cttggccgcg tacctgcgcg gtatcgacgc ctcaatcggc gttcgaccga aagcggtgct
     1561 ggtgatttcg ggtcattggg aaaacccctt gccgacgctc aacattgccg ccaagccggg
     1621 cttactgttc gactactacg gctttccgga ccatacctac cggttgaaat atcccgccgc
     1681 cggcgctccg gaacttgccg aacgtgtgcg caagcttctc gacggcgcgg ggttccagag
     1741 cgacgtggaa tccaaacggg gtctggatca cggtgtattc gtcccgtttc ttctgatcta
     1801 tccgaacgcc gacgtgccca tcctgcaatt gtcgctgcag gccagtctcg atcccggcct
     1861 gcacctggag ttgggcaagg cccttgcgcc gttgcgcgat caggggatcc tgatcgtcgg
     1921 cagcggcatg agctatcaca atttggccga catgttctcc gggcgaggcg ccgaggcagc
     1981 caaaaccttc gatgtctggt tgggcgatgc ggtgcaagat cccgcaacgc gcaatgagtt
     2041 gctcatcaag tggcgcgatg cgccgggtgg tgcagcttcg cacccgcgcg aggaacatct
     2101 catccccttg atggtcgccg ccggcgcggc gcagggtgag cgcggagttc gcagtttcgg
     2161 cgagtcgatc ggcggcaagc agatctcggg ctttcagttt ggctaagaat ccggccgcgc
     2221 tcttggcctg cggtctgctg atcgcggcat gccccgcggt gttcggcgcc gatgcgccga
     2281 ccctcgtgat tcgcgcgcac tcgctgatcg acggcaccgc cgataagccg cggcaaaaca
     2341 tggacgtcgt gattcgcggc aaccgcatcg tcgaggttca tccgacgaat tcgcaggcgc
     2401 tgccagccgc ggtgcaagtc atcgatttag gcgcggcgac cttgctgccc ggcctgatcg
     2461 acacgcacac gcatattttt ctgcagggcg aggatccggc cgccggcggc tacgatgtgc
     2521 aactcttgaa attcccggcg tcgtatcggg ctgcgcgcgc ggtggtggcg gcgcgacgcg
     2581 cgctcgagca gggtttcacc agcattcgcg acatggaaac cgaaggcgca gggtacggcg
     2641 acgtcggcat caagcgagcc atcaacgagg gaatcatccc cggcccgcgc atgtgggtga
     2701 gcacgttgtc gatttcaacg accgggggct atcctctcga gggctacgcg ccggagatcg
     2761 cggcgctgcc gaagggttcc caattgatcg atgggcccat cgaagcacgc aaggcggcgc
     2821 gcgaacaatt ggacaacggc gccgactgga tcaaggtgta catgacccac cgctcctggc
     2881 tcgacgcgca gggggcgctg gtgtcgcagc cgacgctcac ggtcgaagaa ttgaaggcca
     2941 tcgtggacga gacgcacggg tggggaaaga aggtcgcctg tcacgcctac aacggaatcg
     3001 gactgcagcg cgcactcgac ggcggctgcg attcgatcga acacggcctg gaaatcaccg
     3061 atgcgcagat cgcgcagatg ctgaaacagg gtacgtggta cgtgccgacg cttcaggtgt
     3121 actacaccga ttgggatcca gcggacacgg atgccggaaa acgcgaccgc aagcgcgccg
     3181 agttgcacgg cgtgtcgttc ggcaaggcgc tgcgggccgg agtgaagatc gctttcggca
     3241 cggatgtggg gggattcaaa tggtccgagc ccattgccga agagttttca cgagaagtgc
     3301 agctcggcat gacgccgatg caagcgattc agtccgcgac atcgcgggcg gcggagctgc
     3361 tcggcaaacg cggcgaactc ggggtgatcg cgccgggggc gtatgccgat ctcatcgcgg
     3421 tggcgagcga tccgctcgcg gacgtcgcgg tcctcaaggc cgtgcaattc gtgatgaagg
     3481 acggtgtggt gttcaagtcg ccgcgcggca gcgttgcgcc ataggctcag tcctctgccc
     3541 gcaacgtggc gatattttcg gcggtgaccg cggacggttg cgcgccgaat cgcgcggtga
     3601 cgtaattggc gaggctcgcg atctccgcat cggagtaagc actgccaaag gccggcatgg
     3661 cgggccccgc atcggcggta tgacggtggg cgccggtcaa aatgatctgc acgacgttgg
     3721 tcgcggtcgg atcgttcacc gaacgcacgc cggtgagcgt ggcatagggg agcaccggac
     3781 tcacgccgct ccaatcgtgg caccccgcgc acgcgccggc gtacaccgcc cggccgcgcg
     3841 aatccaacgc ggccgccgca ccttgcgagt aagaggaggg cgcaggattt gcttttggct
     3901 ccggcaggtc cgacgtcgcg accgccgtta ccgtgcgcaa ataacggacc atggcgccga
     3961 tgtcgctttg cgtcaggtac tgcaggctgt tgtcgacggc ctcacccatg ggtccggtcg
     4021 ccgtgccgcg cccatcggca tgccccatgg ccagataatg cgcaagatcg gcttcgtccc
     4081 acgtacctat tccgctcttc tgatcagcgg tgatgttgta ggcgcgccat ccggcttgca
     4141 ccgcacctga gaacttcttc cggttatcga gcgcctgcat gagattgcgc ggcgtatgac
     4201 attcgccgca gtgctccatg gcctcgacca gataagcgcc gcggttccag tccggatcgc
     4261 ggccggtgcg cggctcgaag cgcttgtccg gattgaacaa gagcgcccag atacccatca
     4321 gtgagcgctg gttgaacggg aagctcaagg tgttctcggg cgcagccgcg tgcacgggag
     4381 ccaagctgaa cagataggcc ttgatggcga gcgcatcggc gtcggtcatg taggtgtagc
     4441 tcgcgaacgg catggcggga tacagcttgg tgttgttgcg gcctagacct ttgtgaattg
     4501 cgtcgagaaa attcgcatcg ctgtacctgc cgatccccgt ctcggcatcc ggcgtgatgt
     4561 tggtcgagta aagtgtcccg aacggcagca cgaacgcgcg tccacctgcg tagggcgcac
     4621 cgccctctgc ggtgtggcag gcggcacaat ccgcggcacg cgtcagatat tcgcccttct
     4681 ggatcaggct cgcgtctttg agctcgggcg ggacgcccgt cggatcctgc gctcgataat
     4741 cggccaaccg cacgggcgag ccgccggcga agtccatggg gtgcggcgcc aacaccacga
     4801 accaaagaaa accaagcagc atcaccccga gaatcgcggc gatgccgatg aggctgtagc
     4861 gtttcgcgcg gccgcccgag gatgagtcgc tcatgatggt tgcttcctgg acagaatatc
     4921 tcgaccaatg gccacacgcg actatagatc cctcgcacga aatctgccaa tggatcggaa
     4981 tcgagcgcgt gcggccattg ccagcagagc cagcgtcgcc gcgaggcaca ggtaggcaaa
     5041 tagatttccg accagcacgt agatcgtccg gccgccgggc atcccgggcg cggtcacgac
     5101 cacgccatcc tccgcgtgcg gatgccggta cgggtcgtcg gcgtaggttg tttgcagcac
     5161 gcggccattc ggatcgatca gggcggatac gccggaattg accgcgcgca ccatgctcac
     5221 gcgcaattcg acggtggcga acaccgccag cgccaggtgc tcccacggtt cgctatcggc
     5281 gccgaaccac gagtcgctgg tgagattgac caaaaggttc ggatgcagct gcccgacgcc
     5341 gcgcaaaaat ccctgcaaaa tatcctcgta gcaaatcacc ggcccgagtc gccaaccctg
     5401 accgtcgggc tgccgcaggg tgagcacggc cggtccgcgc cccgcggtga atcggccggt
     5461 cccctccggc aggaacttgc gcagccaggg aaaggtctcg atgcccggaa tatattcgcc
     5521 gaacgcaagc aggcgtactt tgtcgtagta gccggcgact cgcccgtcgc catcgagcag
     5581 aatggccgag ttgaaggcgt cctcgtgcgc ctgatcgaac atatcggcgc cgatgaccac
     5641 cggcacgctg aagccgcggc gaatcatcgc cagggagttg ggcgcgaaat cgaccgcgaa
     5701 atctcgcggc agggtgatgg ggtaggcgcc ttcgctccac accacgacat cggcgccggc
     5761 cgcttgcagc cggcgagtct gcgactgcaa agcggagagc tggcgcagaa tctcatcgcg
     5821 agagaattcg cccgtcaccg agtaggcgaa gttcggctgc acgaggccga catgcaatgt
     5881 gggcgcccgc gccaccctgg cgtccacttg ttgcatgcgc accgctccga agatgagcgc
     5941 cgcggccagc agtgccgcgg cggcggccgc gggtatccgc gcggcgcgcc cgtccacggc
     6001 cagatcatag agtgcgccat tgacgagcat caacagggcg gtcacgccaa gcggaccggt
     6061 gagctcggcg atttggatga ccagcgggtg ccaggcttgg gagatccact ggccacaggg
     6121 gaataactgc gggatcacga gttcgcaact caccatccct acgggcgcga gcagggtcat
     6181 cggcacgcgc cgcttggcgc gaatggcgca gacgacggcg gtaaacaaca ggaagatgag
     6241 cgcgcgcgcg gcgcagaata ggaaaaacac cagcaccgcc gcccaccacg gaaagtcggc
     6301 gaagcgctgc atcacatcga tcagccagta aaagccgccc gccgtctcga cgattccggc
     6361 ccaccagccg agcagcaacg cgcgccgaaa attcgccgtg cgatcgattg caaacaacat
     6421 gggcacggcg gcgatccagg ccagcgcgga ataatcgaag ggggtcaccg cgagaaacca
     6481 caggcaaccg ctcgccgtga caaggccgaa tgcgccatag aaattgagct taagcatgct
     6541 taatccgggg tcaatgcagt tcacggtgca tgggcatctc gggtattctg aggcccaatc
     6601 atgcaagaaa gatacgaacc tgccgcggtc gaggcggacg cacaaaattt ttggaatcgg
     6661 cagcgctgct tcgaagcgac ggaagaccaa acccgcgaga agtattattg cttatcgatg
     6721 ttcccctatc caagcggccg cttgcacatg ggacacgtgc gcaactacat gatcggtgat
     6781 gtgctggcgc gattcatgcg catgaacggc aagaacgtgc tgcagccgat gggatgggat
     6841 gcgttcggct tgcccgcgga gaacgcggcc atcgccaacc gcgtgccgcc ggcgaaatgg
     6901 acttcgcaga atatcgcgaa catgaaatcg cagctcaagt ccctgggctt cgcgttggat
     6961 tggagccgcg aactcaccac ctgccatcct gagtactatc gttggaatca atggctgttt
     7021 ctgcgcatgc tggaaaaagg catcgcctac cagaagacgg gagtcgtcaa ttgggatccc
     7081 gtcgacaaga ccgtgctcgc caatgagcag gtgatcgacg gccgcggctg gcgcaccggc
     7141 gcgctggtcg agaaacgcga gatcccgatg tactacctca agatcacggc ctatgccgag
     7201 gatctgctcg aggccttgga tcacatgccc ggttggccgg agcgcgtgcg caccatgcag
     7261 gccaattgga tcggcaagag cgtcggcgtc cgtctcgggt ttccatacga catcgaaggc
     7321 ttgggcgcgg gcgtgatgca tgcgttcacg acgcgtgccg acaccctcat gggcgtgacc
     7381 ttttgcgcgg tcgcgccgga gcatccgctg gcggcttttg cggcgcggag ccacgggaaa
     7441 ttggcggctt tcatcgagga gtgtcagcgc ggtccggcga tcgagtcgca actcgcgacg
     7501 caggaaaaga aaggcatgcc cacgggattg agc
//
LOCUS       DASWZT010000137         7802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_44606, whole genome shotgun sequence.
ACCESSION   DASWZT010000137 DASWZT010000000
VERSION     DASWZT010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7802
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44606"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VGE92_03765"
     CDS             <1..909
                     /locus_tag="VGE92_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312965.1"
                     /translation="LLCSGCASDGARQAAFDRKIQRTEQRLIAAGDADSLAAAAILRI
                     GPKVDPAQRLALIASAVAEAPDRPDLVWLDIQLCILAEGCNPEPIEAQLRAMDPGNGA
                     GWFDSIGRAGQRNDVAAVRQDLRAIATSTRFDIYWNATIAHVTNAILKTHTMDSVTAF
                     ITTTGAASVSAIPAYQTITNACKGDSLQGPDVLETCRQVAAVMRSGDTYITEMIGVAI
                     AKRAWPQGSFEYLDAVGARRVAHYRMEADGKISLHHLWSSRYVATRLRLMAENRTEQE
                     VAKAEMVDAKLNPNPPSEWTDPWAGT"
     gene            894..1967
                     /locus_tag="VGE92_03770"
     CDS             894..1967
                     /locus_tag="VGE92_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY1312966.1"
                     /translation="MGRHVSALLAAPNQAQRLLLLMDHSLEFIEFLGGGGVIQGVSAA
                     IQTLGGYDPADLKGKHYQDIIHPDDCARAATAFAGILRGDVTAPITLRYRHKDGSWRT
                     IQANARNFLSDPAVRAIIVLTRDLTDQLDAEASLAVANLGLRRLSQQLLVVQEKERNR
                     IARELHDDVQQILVGLRMSMESSPRAPGNDLPTNDIETWITLVREAIDHLHALTISLR
                     SPAIGERGLATELRAYIERLVLAEGQDIHTDINADLGSLTPDLELACFRIVQEALANA
                     IKYSGARHLQLSVQRSGHNLTVSVSDDGVGFDLAAARARAIDAGNIGLLSMRERAALV
                     HGSLEVRSSAGQGTRVRATFRTG"
     gene            complement(1964..3124)
                     /locus_tag="VGE92_03775"
     CDS             complement(1964..3124)
                     /locus_tag="VGE92_03775"
                     /EC_number="1.14.11.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethyllysine dioxygenase"
                     /protein_id="HEY1312967.1"
                     /translation="MYRHEAARNEDRVLLSSAELIAGQVVVNWHREQSPAQFSPFWLR
                     DHCHAKHSLNADTLQREVDTFAIPQDIAPQTLEIKDGGAALHILWKHDASTSIFPAQF
                     LWNVAQRDAGPPAPRRTLWDRDSMGKNFPCVSHAEVMGSDAGLLRWLSLVEEFGFALT
                     TGAPVSMAATEALVKRVGYVRETIFGGMWEFSANQAFKDTAYTSVAIGPHTDGTYSND
                     SPGFQMFHCLQFDGSGGDSTLVDGFKVAEAIRNSDRQAFEVLSEVKVPAHYLGDGVHL
                     RAEHPIIGLDQNGDLEQIAYNNYDRAPFRLPPARMAAFYRALALFHRLINDPTYEISM
                     RLTPGTVLFFDNWRTLHGRHAFQGHRHLCGAYLNMEDFQSKLRVLRTRADTP"
     gene            complement(3251..3703)
                     /locus_tag="VGE92_03780"
     CDS             complement(3251..3703)
                     /locus_tag="VGE92_03780"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312968.1"
                     /translation="MSRRAAQSGFTFAETLLTMAFLAIGAGAAVPSVAGHLEHNRVER
                     ATEDIIAIERRLESYRLKHHALPDSLEQLTPAVPLDPWGHAYEYVNFATRGLSEQRSY
                     HNLPVNSDYDLYSRGGDGLTDLVLNADVARDDIVRAGDGDFVGLAANF"
     gene            complement(3706..5472)
                     /locus_tag="VGE92_03785"
     CDS             complement(3706..5472)
                     /locus_tag="VGE92_03785"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEY1312969.1"
                     /translation="MTTTQTFSRALLLHLAALLATQAGAAAPEKSYIVEAHDAAVAAH
                     AVRRVGGHVTHELPIINGASAELTASQAARLGKDSHLQLFADAPVATQDSTISLAQPM
                     IDGTAAVPASSSALASVATAVAAVVSTVVPDIYQRSMMGVNTLAAQGINGAGVVIAVL
                     DSGLLQQGPAYLAADLNGNNRMLAQYDAINNVLVDSHHPCAKAPCVNDDYGHGTHITS
                     LIASSNTADPLLNGNPQGIAPMARLINVKAFNATGVGTYTTVLNGLNWILANRTTYGT
                     IRIVNMSFGAPPQSFYWNDPIDQAVMKLWQAGVVVVASAGNFGPSPQTIAVPGNTPYV
                     ITVGAMTDNFTPNNPSDDGVTSFSSAGPTYEGFVKPDMVAPGGHLAGYMSVNQLLAAE
                     YPQFASEDSKMFIMSGTSQAAAVTSGVVALLLQANPTLSPDQVKCRLLASADAALGAK
                     GKAAYTVFQQGAGRVDAYAAVYSQAANCANQGLNIAADIAGTEHFGGPARQNGNNGNF
                     YVVDSNGNEIKNDGYVWNKQFAKTLGYLWSSGYLWNSGYLWNSGYLWNSSYLWNSGYL
                     WNQNAVTPASTPAAMESWNAQE"
     gene            complement(5636..>7802)
                     /locus_tag="VGE92_03790"
     CDS             complement(5636..>7802)
                     /locus_tag="VGE92_03790"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY1312970.1"
                     /translation="DAAGSIWIANLGTGIDVTDRVHGSTLHYRHVAGDAQSLPDDRVT
                     ALLFDHQDTLWVGTMTAGLSRFDRRTQHFTTYRHDPNDVTSLGAPGVMSLLEDSSGRI
                     WVGTYGGGLSLLNATTGRFIRYTTDPANPETLSSDRATALAEDPSGRIWIGTDGGGLC
                     LLDPISGRVTRFAHEPRNPHSLSANTVYAIRVDQRGQVWVGTRGGGLDEILGSALEPA
                     AIRFRNFSEPEGLPNTTIYGIETGPGGRLWLSTNRGLSRLDPNSHEVRNFRAAHGLQG
                     DEFNFGAHFRSRENELFFGGPQGYNAFFPERLQFDDNPPPVALTAFLKFNEPAPTAVP
                     HEQLRAIDLGYRDDIVTFEFAALDFASPENNQYRYMLEGFDKGWVDAGSKHRVTYTNL
                     AGGNYTFRVRAANSDGKWNEAGLALPLVVQPPPWASKPAYFLYTLAFGLLLFAVWNQQ
                     HKKLQREARYAQRLMQDVRDRTTELAQRNEELERVNRQLKEASVTDPLTGLGNRRYLR
                     DAMAALTKSAEDGLLVGAAHTPVLALLIVDLDHLKPVNDVHGHEAGDQILMQVADILR
                     RNCRTSDFIARWGGDEFVIVYRDEDLSGAELLAERIRSRVAKQIFRLADGKAARTSCS
                     IGFSRYPFVHESPGLLPWEQCLAVADAALYHAKKQRNGWIGWAGTAAAVDVPSILKLL
                     ERDPEALERDGRLDVRRPRFRPEDTVDDMQNRTLRRGDH"
BASE COUNT         1432 a         2387 c         2512 g         1471 t
ORIGIN      
        1 cttctatgta gcggctgcgc gagcgacgga gcccggcaag cagcgttcga tcgaaaaatc
       61 cagcgcaccg agcagcgatt gatcgcggcg ggcgatgccg attccctggc tgctgcggcc
      121 atactgcgca ttgggccgaa ggtcgacccc gcgcagcgct tggcgttgat cgcgagcgcg
      181 gtcgcggagg ccccggatcg tcccgacttg gtctggctcg atattcagct gtgtattcta
      241 gccgagggtt gcaaccctga gccgatcgag gcgcaactgc gtgcgatgga tccggggaat
      301 ggcgccggct ggttcgactc gatcggtcgg gcgggacaac gcaacgatgt cgcggcggtg
      361 cgccaggacc ttcgggccat cgcaacatcc acaaggttcg atatctattg gaatgcgacg
      421 atcgcgcatg tcacgaacgc aatacttaag actcatacca tggattcagt cacggccttc
      481 attaccacca ctggcgcggc gtcggtatcg gccatcccgg cctaccagac gatcacgaac
      541 gcgtgcaaag gcgattcctt gcagggtccc gacgtgctcg agacgtgccg ccaagtggca
      601 gccgtcatgc gcagcggcga tacttacatc accgaaatga tcggtgtcgc cattgccaag
      661 cgagcgtggc cgcaagggag tttcgagtac ctcgatgccg tcggcgcgcg gcgcgtggcg
      721 cattatcgga tggaggccga cggaaaaatc agtctgcatc atttatggag ctcccgatac
      781 gtcgcgacac ggctacgact gatggcggaa aacaggacgg agcaggaggt cgctaaggca
      841 gaaatggtgg acgccaagtt aaatcctaat cccccgtccg agtggaccga cccatgggcc
      901 ggcacgtgag cgcactactc gcagcgccca atcaagcgca acgccttctt ctcttgatgg
      961 accacagtct cgaattcatc gagtttctgg gcggtggggg ggttattcag ggcgtttctg
     1021 cagctatcca gacgctcggc ggctacgatc cggcggatct taagggcaag cactaccagg
     1081 acatcattca tccggatgac tgcgcgcgcg ctgcaaccgc cttcgcgggc attcttcgtg
     1141 gggatgtgac tgcgcccatt acgctgcgct atcgtcacaa agatgggtcg tggcggacaa
     1201 tccaagcgaa tgcccgcaat ttcttgagcg atcctgcggt gcgcgcgatc atcgttctga
     1261 cgcgcgatct gaccgatcag ctcgatgccg aagcatcgct ggcggtggcg aatcttggac
     1321 tgcgtcggct ctctcaacaa ttgctggtgg tgcaagagaa agagcgcaac cgcatcgcgc
     1381 gggaactgca cgatgacgtg cagcaaatcc tagttggttt gcgaatgagc atggagtcct
     1441 cgccgcgagc accgggcaat gatctgccga ccaacgatat cgagacgtgg ataactttgg
     1501 tgcgcgaagc gatcgatcac cttcatgccc tgacgatctc gttgcgatcg ccggctatcg
     1561 gggagcgcgg tttggccacg gagctgcgcg cctacatcga acgcttggta ctggccgaag
     1621 gccaggacat ccacaccgat atcaacgccg atctaggcag cctcacaccc gacctggagc
     1681 tcgcctgctt tcgcatcgtc caagaagcct tggcaaacgc catcaagtac tccggtgcgc
     1741 gacatctgca gctgagcgtg cagaggagcg gtcacaacct gaccgtctcg gtgagcgatg
     1801 acggcgtggg tttcgacctc gcggccgctc gagcacgcgc cattgacgcc gggaacatcg
     1861 ggcttctgag tatgcgagag cgtgcggcac tcgtgcatgg cagcctcgag gtccgctcct
     1921 cggccgggca aggaacccgg gtgcgcgcca cattccgcac gggctaaggc gtgtcggcgc
     1981 gcgtgcgcaa cacccgcagc ttgctttgga aatcttccat attgaggtag gcgccgcaca
     2041 ggtgccggtg tccctggaac gcatgacggc cgtgcaaggt gcgccagttg tcgaaaaata
     2101 gcaccgtccc cggcgtgagg cgcatcgaga tctcatatgt cggatcgttg atcaggcgat
     2161 gaaacaatgc caacgcgcgg tagaacgccg ccatgcgcgc cggcggcagc cggaacggcg
     2221 cgcgatcgta attgttgtag gcgatttgct caagatcgcc gttctgatcg agacctatga
     2281 tgggatgctc ggcccgtagg tgaacgccgt cgcccagata atgggcgggc accttgacct
     2341 cactcaacac ctcgaaggct tggcggtcgc tgttgcgaat cgcctccgcc accttgaagc
     2401 cgtcgaccaa ggtactgtcg ccgcccgagc cgtcgaattg caagcaatga aacatttgaa
     2461 agccgggcga gtcgttgctg tacgtgccgt cggtgtgcgg gccgatggcg accgacgtat
     2521 aggccgtatc cttgaacgcc tggttggcgg agaattccca cataccgccg aatatcgtct
     2581 cgcgcacgta gcccacgcgt ttcaccagcg cctcggtcgc cgccatcgat accggcgccc
     2641 cggtggtcaa ggcgaagcca aattcctcga ccagggaaag ccaacgcagc aagccggcat
     2701 cggagcccat cacttcggcg tgactcacgc acgggaaatt cttccccatg gaatcgcggt
     2761 cccacaaggt gcggcgcggc gccggcggcc ctgcgtcgcg ttgtgcgacg ttccagagaa
     2821 actgggccgg aaaaatgctt gtgctggcgt cgtgcttcca gaggatatgc agagctgcgc
     2881 cgccatcctt gatttcaagc gtttgcggcg cgatgtcctg cggtatcgca aaggtatcga
     2941 cctcgcgctg cagcgtgtcg gcgttcaagc tgtgcttggc gtgacagtga tcgcgcagcc
     3001 agaagggcga gaactgcgcc ggcgactgtt cccggtgcca gttcaccacc acctgtcccg
     3061 cgatcagctc ggccgagctc aacagcacac ggtcctcgtt tcgcgccgct tcgtgtcggt
     3121 acatgcaagt tctcgctgtg tcttccctgg taattgaaca atcgtatagt aaggaatgga
     3181 gcgcgtcaaa tggccgcggc gcgcgtcgcc atcaatcgct tgggagagag cgcagggcgc
     3241 gcctgcggcg ctagaaattc gcggccagcc ccacgaaatc gccgtcgccg gcgcgcacga
     3301 tgtcgtcgcg ggcgacatcg gcattcaaca cgagatcggt gaggccgtcg ccgccgcgcg
     3361 aatacagatc gtaatcgctg ttgacgggca gattgtggta ggaacgctgt tcgctcaagc
     3421 cgcgggttgc gaaattcaca tattcgtagg cgtgtcccca aggatccaac ggcacggcgg
     3481 gggtcagctg ctcgagcgag tcgggcagcg cgtgatgctt aaggcggtag gactcgaggc
     3541 gccgctcgat ggcgatgatg tcctcggttg cacgctcgac gcggttgtgt tcgagatggc
     3601 cggcgacgct cggaaccgcg gcacccgcgc cgatggcgag aaacgccatg gtgaggagcg
     3661 tttccgcgaa cgtgaatccg gactgggcgg cgcgtcgcga catggttatt cctgcgcgtt
     3721 ccaagattcc atcgcggccg gcgtggacgc cggcgtcacg gcgttctggt tccacaagta
     3781 gcccgagttc cacaggtagc tgctgttcca caggtaaccg ctgttccaca ggtagccgct
     3841 gttccacaag tagccgctgc tccagaggta gcccaaggtt tttgcaaact gcttgttcca
     3901 aacgtagccg tcgttcttga tctcgttgcc gttgctgtcg acgacataga agttgccgtt
     3961 gttgccattc tgccgcgccg ggccaccgaa atgctcggtg cccgcaatat ccgccgcgat
     4021 gttcaatcct tggttggcgc agttggcggc ctggctatag accgccgcat acgcatcgac
     4081 gcgtcccgcg ccctgttgga acacggtgta agccgccttg cctttggcac cgagcgccgc
     4141 gtcggccgag gccaacagcc gacacttgac ctgatccggg gacaaagtcg gattcgcctg
     4201 caacagcagc gcaacgacac cgctggtaac ggccgccgct tgcgaggtgc cggacatgat
     4261 gaacatcttc gagtcttccg aggcgaactg cgggtactcg gccgccagca gctgattcac
     4321 gctcatgtat ccggcaagat gaccgcccgg agcgaccatg tcgggcttga cgaagccttc
     4381 gtaggtcggg ccggcggatg agaacgacgt aacgccgtca tccgacggat tgttcggcgt
     4441 gaagttgtcg gtcatcgcgc cgacggtgat gacatacggg gtattgcccg gcacggcgat
     4501 ggtctgcggc gacgggccaa aattgcccgc cgaggcgacc acgaccacgc cggcctgcca
     4561 cagcttcatc actgcctggt cgatcggatc gttccagtaa aaagactgcg gcggcgcgcc
     4621 gaacgacatg ttgacgatgc ggatcgtgcc gtaggtggtg cgattggcca gtatccagtt
     4681 caagccgttg agcacggtcg tataggtgcc gacgccggtg gcgttgaaag ccttgacgtt
     4741 gatcaaccgc gccatcggcg cgattccttg ggggttgccg ttcaacaagg gatcggcggt
     4801 gttcgaactc gcaatcaggc tggtgatgtg cgtgccatgg ccgtagtcgt cgttcacgca
     4861 cggcgccttc gcgcaggggt ggtgcgaatc gaccagcaca ttattgatcg catcgtattg
     4921 agcgagcatg cggttgttgc cgttcaagtc cgcggcgaga tacgccggtc cctgttgcag
     4981 caggccgctg tcgagcactg cgatcacgac gccggcgccg ttgatgccct gcgcggcaag
     5041 cgtgttgacg cccatcatgg agcgttggta gatgtccggg accacggtcg ataccaccgc
     5101 ggcaacggcc gtggccacgg atgcgagcgc cgaggagctc gccggcaccg cggccgtgcc
     5161 gtcgatcatg ggctgcgcca agctgatggt ggagtcctgc gtggccacgg gcgcatcggc
     5221 gaagagttgc agatgagagt ctttgccgag gcgtgccgcc tggctcgcgg tgagctcggc
     5281 cgaggcgccg ttgatgatgg gcagctcatg cgtgacatga ccgccgacac gccgcaccgc
     5341 gtgggccgcc accgccgcgt cgtgtgcctc cacgatgtag cttttctccg gcgccgcagc
     5401 gcctgcctgg gtggcaagca gggctgccag atggagcagc agggctcggg aaaatgtctg
     5461 tgtcgtggtc acttgcgccg ccttcgtcaa tgcttcgccc ggtatcgggc acggcgctat
     5521 ttcaactgag cgggggattt gccgggctta ctggctcgac tgccaccagc ccgccaagtg
     5581 cgaacgggtt cgcataatcc ggcggttaaa tacggctgcg accggccatt ctgcgttaat
     5641 ggtcgccgcg gcgcagggtc cgattttgca tatcgtcgac cgtgtcctcg ggacggaatc
     5701 tcggtctcct gacatcgagc cggccgtcgc gctcgagcgc ctccggatct cgttcgagca
     5761 atttgaggat cgacggcaca tcgacggccg ctgccgtgcc ggcccagccg atccagccgt
     5821 tacgctgctt tttcgcgtgg tagagcgcgg catcggcgac cgccagacat tgctcccagg
     5881 gcaggaggcc cggcgactcg tgcacgaatg gatagcgcga gaagccgatc gagcaactgg
     5941 tgcgggcggc tttcccgtcc gccagccgga agatctgttt ggcaacgcgc gagcggatgc
     6001 gctcggccag cagctccgcg ccgctcaaat cttcatcgcg atacacgatg acgaactcat
     6061 cgccgcccca gcgcgcgatg aaatcggagg ttctgcaatt gcgccgcaga atgtccgcga
     6121 cttgcatcag aatctgatca cccgcctcgt ggccgtgcac gtcgttcacc ggcttcaaat
     6181 ggtcgagatc gacgatcagc aatgccagca ccggcgtgtg cgcggctccg accagcaggc
     6241 cgtcctccgc gctcttggtc aatgccgcca tcgcgtcgcg caagtagcgc cggttgccga
     6301 ggcccgtcaa aggatcggtg acgctcgctt ccttgagctg gcgattgact cgctcgagtt
     6361 cttcgttgcg ctgcgcgagc tcggtggtgc gatcgcgcac atcctgcatc agccgttgtg
     6421 catagcgcgc ctcgcgctgc agcttcttgt gctgttggtt ccagacggcg aacagcagca
     6481 agccgaaagc gagcgtgtac aagaagtagg cgggcttcga tgcccacggc ggtggttgaa
     6541 ccaccagcgg cagggcgagc cccgcctcgt tccatttgcc gtcactgttg gcggcgcgca
     6601 cgcggaaggt gtagttgccg ccggcgagat tggtgtaggt cactcgatgc ttcgagccgg
     6661 cgtcgaccca acccttgtcg aagccctcca gcatatagcg gtactgattg ttttctggcg
     6721 aggcgaaatc gagcgccgcg aattcgaatg tcacgatgtc gtcgcgataa ccgagatcga
     6781 tggcgcgcag ctgctcgtgc ggaacggccg tgggcgcggg ctcgttgaat ttcaagaacg
     6841 cggtcaatgc caccggcggc gggttgtcgt cgaactgcag ccgctcgggg aagaaagcgt
     6901 tgtagccctg cggtccgccg aaaaacaatt cgttctcgcg gctgcgaaaa tgcgccccga
     6961 agttgaattc atcgccttgc agcccgtgcg cagcgcgaaa atttcgaact tcgtggctgt
     7021 tggggtcgag tcgcgacaga ccgcgattgg tgctgagcca cagccgcccg ccgggtccgg
     7081 tttcgatgcc gtagatcgtc gtgttcggca agccttcggg ctcggagaaa ttgcggaatc
     7141 ggatcgctgc cggctcgagc gcggaaccta agatctcatc gaggccgccg cctcgagtgc
     7201 ccacccacac ttgaccgcgc tgatcgacgc ggattgcgta cacggtgttg gcgctcaggg
     7261 agtgcgggtt gcgcggttca tgcgcgaagc gcgtgaccct gccgctgatc gggtcgagca
     7321 ggcacagtcc gccgccgtcg gtgccgatcc agatgcgccc ggaagggtcc tcggccaagg
     7381 cggttgcccg gtcgctcgat aaggtttccg ggtttgcggg atccgtcgtg tagcggatga
     7441 aacggccggt ggtcgcattc aagagcgaca gcccaccgcc ataggtgccc acccagatcc
     7501 ggccgctcga atcctcgagc agcgacatca ccccgggggc gcccaagctc gtaacgtcgt
     7561 ttggatcgtg acggtaggtc gtgaaatgtt gcgtgcgacg atcgaagcgg ctcaagcccg
     7621 ccgtcatcgt tcccacccac aaggtgtcct gatgatcgaa cagcagggcg gtgacgcgat
     7681 cgtccgggag actttgagca tcgcccgcca cgtggcggta atgcagcgtg gagccgtgca
     7741 cccggtccgt gacgtcaatg ccggtgccca agttggcaat ccaaatcgaa ccggccgcgt
     7801 cc
//
LOCUS       DASWZT010000138         5423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_83170, whole genome shotgun sequence.
ACCESSION   DASWZT010000138 DASWZT010000000
VERSION     DASWZT010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5423
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83170"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="VGE92_03795"
     CDS             complement(<1..1082)
                     /locus_tag="VGE92_03795"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY1312971.1"
                     /translation="MSGSRLAAPAGQRLDRSRRLTFQFNGRTLYGHPGDTLASALLAN
                     GVQLVGRSFKLHRPRGIFSCGVEEPTGLVDVGEGATRTPNLRATLVELHDGLIAASVN
                     CWPSVGFDLGAVNSRLGALLPAGFYYKTFKWPNWHWYEPAIRRMAGLGRASSVPDPDR
                     YEEVAAQVEVLVVGGGPAGLTAAVSAAEAGAKTLLVTSGPALGGALAWRRDPHIEQLM
                     LRAARCGLEQMTRGLAFGVYDHNLVCVRESLPGAAGAKGGVLRERLWKIRARAVIAAT
                     GALERPMIFPDNDRPGVMLAGAADKYAHAFGVACGRRVVIAANSDAAYQVAASLRALG
                     IEVVALIDRRARSDIAAAAAVRTLTNA"
     gene            complement(1079..1342)
                     /locus_tag="VGE92_03800"
     CDS             complement(1079..1342)
                     /locus_tag="VGE92_03800"
                     /inference="COORDINATES: protein motif:HMM:NF016179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEY1312972.1"
                     /translation="MLQIPCIHCGLRDENEFTCGGTSHLTRPALQASDATWGDYLFFR
                     DNPKGLHFERWRHSFGCGQWFNIARDTVTHEIHAVYAITAAPP"
     gene            complement(1342..2595)
                     /locus_tag="VGE92_03805"
     CDS             complement(1342..2595)
                     /locus_tag="VGE92_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008365064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HEY1312973.1"
                     /translation="MQNKYSAWSLLRGALSGQRHWTAAWRDATPKAGYDVIIVGGGGH
                     GLATAYYLARNHGVTRVAVIERGWIGGGNTGRNTTIVRSNYLYPESARLYDFSLKLYE
                     GLSQELNFNIMLSQRGLILLAHSRHDLDAMSRWANAMRMNGIEAQLLSRSEVRELVPA
                     LDLSPQARLPVLGGFTQRRGGTARHDAVAWGYARAASALGVDIIQNCEVVDFIKESGG
                     IVGVNTNRGAIRADRVGIAAAGHSSVIAKLAGFRLPVTSYALQAMVSEPVKPVLDTVV
                     LSPGTGAYVSQSDKGEIVMGAMLDLFPSYAQRGSFAVKQAIIGATLELFPSFSRLHLM
                     RRWAGIVDVVHDSSPIIGPTPIANLFINCGWGTGGFKAIPAGGWTLAHVLATGQSHPL
                     AEPFQLQRFITGRLIDEAAASGIAH"
     gene            complement(2585..3811)
                     /locus_tag="VGE92_03810"
     CDS             complement(2585..3811)
                     /locus_tag="VGE92_03810"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY1312974.1"
                     /translation="MNARDTVLGRLRGRNLETALERDFYCSPEDYQVDLEMIWYRDWL
                     FVGHDCEVPSPGNYFTVQIGEYPVIVVRDREGGLRAFHNTCRHRGSRICSAERGTAPR
                     LVCPYHQWTYALDGRLIAARDMGGEFDKTQHGLKPVHCASVAGYIWICLAAAAPDFEP
                     IRQHIEPYLLPHKLHQTKIAFQSTIIEQANWKLVWENNRECYHCAANHPELIRTFPED
                     PTATDGEDAASNPKTAARWAHWESIGLPSKFHLSANGQYRTLRTPLLEGAVSFTMTGK
                     AAVRRPLSDSVAEPDIGTLLMFHFPTTWNHVLADHAISFRVLPISPTQTQLTTKWLVH
                     RDAVEGVDYDVNELTRVWLATNAADTVNCQENQIGVNSPAYAPAPYSPVHEAGVTQFV
                     HWYCTHLEGRLLQDAK"
     gene            3946..>5423
                     /locus_tag="VGE92_03815"
     CDS             3946..>5423
                     /locus_tag="VGE92_03815"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY1312975.1"
                     /translation="MAPSPNALARCHAKTLLGALLACWLLALGGAACAAPVPASLRVG
                     NIELRRCPRVPAYCGTLDRPLDPTGVVPGRISIYFEYYPPIGPGPASNTLVATEGGPG
                     YSTTESRADYLTLFGPLRQRRAVLLMDNRGTGQSGAIDCHELQTAEKMTIELIAACGR
                     SLGDRAPLYSTAYASDDLAAILDALEIGRIDMYGDSYGTYLEQVFAVRHPARLRAIVL
                     DGSYPLNGPDYAWYPQYAPAMRDKFNIACRRFAPCAELPGDSIAHILPALQELRSKPF
                     SARAADGAGRPREFMANASQLAIVMFGGAPALATVREGDAAARAFVAGDRTPLLRLMA
                     ETDSAVDSRDPTADATKWSAGLGAVVLCHDPPQIFDMRLAPALRAADRDRAIEERKRT
                     LPGTYAPFSIDEYRGMQLDYSFIDLCVAWPVAPSSHPASHVVGADAQYPQIPALIISG
                     EFDNMTTGTDGAAVAAAFRHGIQIRIANSFHVNALPRARSTCA"
BASE COUNT          983 a         1814 c         1666 g          960 t
ORIGIN      
        1 gcattggtca aggtgcgcac cgccgcagcg gcggcaatat cgctgcgcgc acgccgatcg
       61 atcagcgcca ctacctcgat gccgagtgcg cgcagcgagg ccgccacttg ataagcagca
      121 tcgctgttgg ccgcgatcac cacccgccgg ccgcaggcga cgccgaaggc gtgcgcgtat
      181 ttgtccgcag caccggccaa catcacgccc ggacggtcgt tatccggaaa aatcatcgga
      241 cgctcgagcg cgccggtcgc ggcgatgacg gcgcgcgcgc ggattttcca cagccgctcg
      301 cgcagcacgc cgcccttcgc cccggccgcg cccggcaacg actcgcgcac gcacacgagg
      361 ttgtgatcgt acacgccgaa cgccaagccg cgcgtcatct gttcgagccc gcagcgcgcg
      421 gcgcgcagca tgagttgctc aatgtgcgga tcgcggcgcc aagcgagggc accgccgagt
      481 gcagggccgc tcgtaaccaa cagcgtcttg gcgcccgcct cagccgcgct caccgcggcc
      541 gtaaggccgg cgggaccgcc gccgaccacg agcacttcga cctgtgccgc gacttcctcg
      601 tagcgatcgg ggtcgggcac gctcgaggcc ctgccgaggc ccgccatgcg ccgaatcgcc
      661 ggttcgtacc aatgccagtt cggccacttg aaggtcttgt aataaaagcc cgccggcaac
      721 agcgctccca gccggctgtt gaccgcgccc aagtcgaaac cgacgctcgg ccagcaattg
      781 acgctggccg cgatcaggcc gtcgtgtaat tccacgagcg tggcacgcag attcggcgta
      841 cgcgtggcac cctcgcccac atccaccagg cccgtcggct cttcgacgcc gcacgaaaaa
      901 atgccgcgcg gccgatgcag cttgaagctg cgccccacca gctgcactcc gttcgccaac
      961 agcgccgatg ccagcgtatc acccggatgg ccgtacaaag tgcggccatt gaattggaat
     1021 gtcagccgcc gcgatcgatc gagacgctgt cccgcgggcg ccgccaaacg cgagccgctc
     1081 acggcggcgc agccgtgatg gcgtagactg cgtgaatttc gtgggtcacg gtgtcgcgcg
     1141 cgatgttgaa ccattgacca cagccgaacg agtgacgcca gcgttcgaaa tgcagtccct
     1201 tcgggttgtc gcgaaagaac agatagtcgc cccaagtcgc atcgctcgcc tgcaacgccg
     1261 gccgcgtcag gtgcgaggta ccgccgcagg tgaattcatt ctcatcgcgc agtccacagt
     1321 gaatgcaagg aatctgcagc atcagtgtgc gattcccgat gccgctgctt cgtcgatcag
     1381 gcggccggtg atgaagcgct gcagttgaaa gggctcggcc aggggatggc tctgtcctgt
     1441 cgccagcacg tgggccagcg tccagccgcc ggcgggaatc gctttgaagc cgccggtacc
     1501 ccagccgcaa ttgatgaata aattcgctat cggcgtcggg ccgatgatgg gactcgaatc
     1561 gtgcaccaca tcgacgatac cggcccaccg gcgcatgaga tgcaaacggc tgaaggacgg
     1621 gaacaactcg agagtggcgc cgataatggc ctgcttcacg gcaaaactgc cgcgctgcgc
     1681 atacgaggga aagaggtcga gcatggcgcc catcacgatt tcgcctttgt cggactggct
     1741 cacataggcg ccggttccgg gggacaggac caccgtatcg agtacgggtt tgaccggttc
     1801 gctcaccatg gcctgcagcg cgtagctcgt caccggtagc cgaaagccgg ccagcttcgc
     1861 gatcacggag ctatggcccg ccgcggcaat gccgacccgg tcggcacgga tcgcaccgcg
     1921 attggtgttc acgccgacaa tgcccccgct ctccttgatg aagtcgacga cttcgcaatt
     1981 ttggatgatg tcgacgccca aggctgaagc cgcccgcgcg tagccccagg ccaccgcgtc
     2041 gtgccgggcc gttcctccgc gccgctgagt aaatccgcca agcaccggca gccgtgcctg
     2101 tggcgataaa tcgagtgccg gcaccagctc gcgtacctcg cttcggctca gcagctgcgc
     2161 ctcgatgccg ttcatgcgca tggcattggc ccagcgcgac atggcgtcca aatcatgccg
     2221 gctgtgagcc agcaatatca ggccgcgctg gctcaacatg atgttgaagt tcaattcctg
     2281 cgacagccct tcgtagagct tgagggagaa gtcatacaag cgcgcgctct cggggtacag
     2341 ataattggag cgcacgatgg tggtattgcg gccggtgttg ccgccgccga tccagccgcg
     2401 ctcgatgacg gcgacccgcg tgacaccatg gtttcttgcc aggtagtagg cggtggccaa
     2461 cccgtgaccg ccgccgccga caatgatcac atcgtaaccg gccttgggcg tcgcgtcgcg
     2521 ccaagccgcc gtccagtgac gctgcccgga caacgcgccg cgcagcaacg accaggccga
     2581 atatttattt tgcatcctgc agaagtctgc cctcaagatg cgtgcaatac cagtggacga
     2641 attgagttac gccggcctcg tgcaccggtg aatagggcgc cggagcatac gccggcgagt
     2701 tcacaccgat ctgattttcc tggcagttca ccgtatcggc cgcgtttgtg gcgagccaca
     2761 ccctggtcaa ttcgttgaca tcgtagtcga cgccttcgac ggcatcgcga tgcaccagcc
     2821 atttggtcgt caattgcgtc tgcgtcgggc tgatcggcag aacccgaaac gaaatcgcat
     2881 ggtcggcgag cacgtgattc caggtcgtcg gaaaatggaa catcaggagc gtgccgatat
     2941 ccggttccgc gaccgaatcc gacagcggcc ggcgcaccgc ggcctttcct gtcatggtaa
     3001 agctcaccgc gccttcgagc agcggcgtgc gcaaggtacg atactggccg ttcgcagaga
     3061 gatgaaattt gctcggcagg ccgatcgact cccaatgcgc ccagcgcgcg gctgttttgg
     3121 gattcgaggc cgcatcctcg ccgtcggtcg cggttggatc ttccggaaac gtccggatga
     3181 gctcgggatg attggcggcg cagtgataac actcgcggtt attctcccag accaatttcc
     3241 aattggcttg ttcgatgatc gtcgattgga aggcgatctt ggtctggtgc aatttatgcg
     3301 gcagaaggta cggctcgatg tgctggcgaa tcggctcgaa gtccggcgcc gccgccgcca
     3361 ggcaaatcca gatgtaaccg gcgacgctcg cgcagtgcac gggtttcaag ccatgctgcg
     3421 tcttgtcgaa ctcgccgccc atgtcgcgcg ccgcgatcaa tcgcccgtcg agcgcatagg
     3481 tccactggtg atagggacaa accaagcgcg gcgccgtgcc gcgctctgcg gagcaaatcc
     3541 tcgatccgcg gtgcctgcag gtgttgtgaa aggcgcgcag gcccccctcc cggtcgcgta
     3601 ccacgatcac cggatactcg ccgatctgca ccgtgaagta gttccccggg ctgggcacct
     3661 cgcagtcatg cccgacgaac agccaatcgc ggtaccaaat catctcgaga tcgacctggt
     3721 aatcctctgg gctgcaatag aaatctctct cgagggcagt ttcgagatta cgtccgcgta
     3781 atcggccgag tacggtgtcg cgtgcattca tggcgcagat cctgtgtcga tgcacgtacg
     3841 atagcaatat tcgaacccgc gccaatgccc cgaagcgacc ctttttacgc aataaacgac
     3901 agcgcgattt tgcgcccgca ccgggagtgc gtagactttc gcgccatggc gccctcgcca
     3961 aatgcactcg ctcgctgcca cgccaagaca ctgctcggtg cgctgctggc gtgctggctg
     4021 ttggcgttgg gcggagccgc ttgcgctgcg cccgtgcccg cttcgcttcg ggtgggaaac
     4081 atcgaacttc ggcgctgccc cagggttccc gcctattgcg gcactctcga ccggccgctc
     4141 gaccccactg gtgttgtccc cggccgcatc tcgatctatt tcgagtacta cccgccgatc
     4201 ggacctggcc ctgcgtcgaa cacgctggtc gccaccgagg gagggcccgg ctattcgacc
     4261 accgaatcgc gcgctgacta cctgactcta ttcgggccat tgcgtcagcg ccgtgccgtg
     4321 ctgctgatgg acaaccgcgg gaccggtcaa tccggggcca tcgattgtca cgaactgcag
     4381 acggccgaaa agatgaccat cgagttgatc gccgcatgcg gtcgatcgct cggcgaccgc
     4441 gcgccgctct acagcactgc gtatgcttcg gatgatctgg ccgcgattct cgatgcgctc
     4501 gagattgggc gcatcgatat gtacggagat tcgtacggga cctatctcga acaggtcttc
     4561 gcggtgcgtc atccggcgcg gctgcgcgcc atcgtactcg acggcagtta tccgttgaac
     4621 ggaccggact atgcctggta tccgcagtac gcgccggcca tgcgcgacaa attcaatatt
     4681 gcctgccggc gcttcgcgcc gtgcgcggag cttcccggcg actcgatcgc gcacatcctg
     4741 ccggcgttgc aggagttgcg ctccaagccc ttctccgcgc gggcggcaga cggcgcaggc
     4801 aggccgcgag aattcatggc gaacgcctcg caactcgcca tcgtgatgtt cggcggcgcg
     4861 ccggccttgg ccacggttcg cgaaggcgac gcggcggcgc gcgccttcgt tgcgggcgac
     4921 cgcactccgt tgctgcggct catggcggaa accgacagcg ctgtggattc ccgagatccc
     4981 accgccgacg ccacgaaatg gagcgcgggt ctcggggccg tcgttctgtg ccacgaccca
     5041 ccgcaaatct tcgacatgcg tctggcgccc gccctgcgcg ccgccgatcg cgaccgcgcc
     5101 atcgaggaac gcaagcgcac gctgcccggc acgtacgcgc cgttcagcat cgacgaatac
     5161 cggggcatgc agctcgatta cagcttcatc gacctgtgcg ttgcctggcc ggtcgctccc
     5221 tcgagtcacc ctgcctcgca tgtggtcggc gcggatgcgc aatacccgca aatacccgca
     5281 ctcatcatct ccggcgagtt cgacaatatg acgaccggca cggatggcgc cgcggtggcc
     5341 gctgcattcc ggcatggcat ccaaattcgc atcgccaaca gtttccacgt caatgcgctg
     5401 ccgcgggcac gcagcacctg cgc
//
LOCUS       DASWZT010000139         2775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_73595, whole genome shotgun sequence.
ACCESSION   DASWZT010000139 DASWZT010000000
VERSION     DASWZT010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73595"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(447..1658)
                     /locus_tag="VGE92_03820"
     CDS             complement(447..1658)
                     /locus_tag="VGE92_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HEY1312976.1"
                     /translation="MARPTLEVADIFRDHGPGWREANRGHVSLGQLKVMGAIEHCRTA
                     ALGGHVARCENAACGHTEISYNSCRNRHCPKCQGAASRRWLAEREAELLPVPYFHVVY
                     TLPSQLRDIAYQNKSVVYNLLMKAAAETTLAIASDPKRLGARIGVTAVLHSWGSALTH
                     HPHVHMIVPGGGLSFDNARWVAARPDFLVHVKVLAHLFRGKMLALLMAAHKANQLTFF
                     NSHAGLADKKTFKRFIAPLRRIKWVVYCKAPFAGPEQVLRYLSRYTHRVAISNRRLVA
                     ADDGAIAFRWKDYRLDGPDRWKTMRLHPHEFIRRFLIHVLPKGFHRIRHYGLFANTNR
                     AENITTARALLNVAPPAAEPQQPPDIATDTPRVLPCPCPRCGGRMIVIETFAPGCEPK
                     WRPTPNRIDTS"
     gene            complement(1668..2594)
                     /locus_tag="VGE92_03825"
     CDS             complement(1668..2594)
                     /locus_tag="VGE92_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY1312977.1"
                     /translation="MSDANIVSPLRRRMIEDMTARKLNPHTQRSHLQSCRRFAAWLKR
                     SPDTATPDEVRQFQLHLIESGTSICNRNRIMTGVRFLFRVTLRRHDLAAEVWHIREPV
                     RLPPVLSPEEVKRVLTMATSLKARAMLSLAYGCGLRSSEVVRLRVGDIDREQKIIRIV
                     QSKGRKDRYVMLPAEVLVLLRQWWKARPSADDAGIAPEQRWLFPGRSDHRPLTTRQFG
                     RLFKEAAKAAGLRKTVSLHSLRHSFATHLLERGKDIRVIAALLGHDRLETTARYSRVA
                     IGMIAGIESPLDALSKPGRGPARRDQEKPPAP"
BASE COUNT          496 a          838 c          913 g          528 t
ORIGIN      
        1 gatcatgaaa ggatgcatcg aagctggggc gaagtgagga gcgcgttttg gcagcgatgc
       61 ttctcaccgg aactgcatgg atgtctcttc gtgtggaggt tttcggccgc cgggatgctg
      121 gtctatccac acgctgaggc agccggcggc cgaaaagcgc caaggcacga agtcgcggga
      181 ggttgagcac cagcggggca gcgggcgcta tggggattat atggcggcgc cgagctcggg
      241 ggttgatgcg cggctgttgg aatacggctg gcggatccgc ccacgagtga gatcgtaagc
      301 ctgtgacagg cgtttcggca agcgtttgga actcatcaac gagcgcacga tgggttgacc
      361 acggcgattg tggcgagagg tatcgttgcc ggcgtaggac cagcgcatca gaacagggag
      421 ttcgcgacgc tcacgggagg tctgggtcat gatgtgtcga tcctgttcgg cgtcgggcgc
      481 cacttcggct cgcagccggg cgcgaaggtc tcgatcacga tcatgcggcc gccgcagcgc
      541 ggacaagggc aaggcagcac ccgtggcgtg tcggtggcga tatccggcgg ctgttgcggt
      601 tcggcggcag gcggggcgac attgaggagc gcgcgggctg ttgtgatatt ttcggcgcgg
      661 ttggtgttgg caaagagccc gtagtggcgg atgcggtgga agcccttggg cagcacgtgg
      721 atcagaaaac gtctaatgaa ctcgtgcggg tgaagccgca tcgtcttcca gcgatcgggg
      781 ccgtcgaggc gataatcttt ccaccggaac gcaatcgcgc cgtcgtcggc tgcaacgagg
      841 cggcggttcg agatggcgac gcgatgggtg taacgagaga ggtagcgcag gacctgctcg
      901 ggccctgcga atggcgcctt gcaatagacc acccacttga tgcgccgcag cggggcgatg
      961 aaacgcttga atgttttctt gtcggcgagt ccggcatggc tgttgaagaa cgtcaattga
     1021 ttggctttgt gcgccgccat gagcagggcc agcatcttgc cgcggaacag gtgcgccagc
     1081 accttgacat ggacgaggaa gtcgggacgc gcggcgaccc atcgcgcgtt atcgaacgag
     1141 aggcctccac ctggcacaat catgtgcacg tgcggatgat gggtgagcgc tgagccccag
     1201 ctgtgcagca cggcggtgac accaatcctt gcgccgagac gcttgggatc gcttgcgatg
     1261 gcgagcgtcg tctcggccgc ggccttcatc agcaggttat agaccacgct tttgttctgg
     1321 taggcgatgt cgcgcagctg gctcggcagg gtgtagacga cgtgaaaata cggcaccggc
     1381 aacagttccg cttcgcgctc ggccagccat cggcgtgacg ccgcaccctg gcacttcggg
     1441 cagtgccggt tacggcagct gttataactg atctcggtat ggccgcacgc cgcgttctcg
     1501 cagcgcgcga catgcccgcc gagcgctgcc gtacgacagt gctcgattgc acccatcact
     1561 ttcaattggc cgaggctcac atgcccgcgg ttggcctcac gccagccggg accgtgatcg
     1621 cggaagatat ccgcgacctc gagagttggt cgagccatgg ccggcggcta tggcgctggc
     1681 ggcttctcct ggtcccgcct cgcaggcccg cggcccggct tgctcagggc atcgagtggg
     1741 ctctcgatcc ccgcgatcat tccgatcgct acgcgactgt agcgggctgt cgtctcaagc
     1801 ctgtcgtgcc cgagcaaagc tgcaatcaca cgaatatcct tgccgcgctc gagcagatgg
     1861 gttgcgaagc tgtggcgcag cgaatgcagc gagaccgtct tgcgcagccc cgcggcttta
     1921 gcggcctcct tgaacagccg gccgaactga cgcgtggtca gtggccgatg atcactgcga
     1981 cccggaaaca gccagcgctg ttccggggca atgccagcgt catcagcgct cggccgcgcc
     2041 ttccaccatt gccgcaacaa aaccaggacc tccgccggca gcatcacgta tcggtcctta
     2101 cggcccttcg actgcacgat gcggataatc ttctgctcac gatcaatatc acccactcgc
     2161 agccgcacca cctcgctgga ccgcagtccg caaccatagg cgaggcttag catcgctcgc
     2221 gccttcaaac tcgtcgccat ggtcagaaca cgcttgacct cctcggggct cagcaccggc
     2281 ggcagccgca cgggctccct gatgtgccag acctcggccg ccagatcgtg gcgacgtagc
     2341 gtgacgcgga acaagaaccg cacccccgtc ataatccgat tgcggttaca gatgctcgtc
     2401 ccactctcga tgaggtgcag ttggaattgg cgcacctcgt cgggcgttgc cgtgtcgggc
     2461 gagcgtttaa gccaagcggc gaaccgccta cagctttgca ggtgactgcg ctgcgtatgt
     2521 gggttgagct tgcgcgcagt catatcttcg atcattcttc gacgcagcgg gctgacaatg
     2581 ttggcatcgc tcatggggag ctccattctg agtgaggttc ctgaaacacc ctcaattctc
     2641 aggacggagc tctccagcgc tagggctatc ctcgactacc gacccctcgg ctcttgctcc
     2701 cgcgcagcgg gctggagcgc agcgactttg tgcttgggtc aatcgcgtcg gtttgaccct
     2761 aggccagcca cttcc
//
LOCUS       DASWZT010000140        11783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_92990, whole genome shotgun sequence.
ACCESSION   DASWZT010000140 DASWZT010000000
VERSION     DASWZT010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11783
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92990"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VGE92_03830"
     CDS             <1..235
                     /locus_tag="VGE92_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009683443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312978.1"
                     /translation="HYTRPAAIDGATVPAVLMSGDHAAIRRWRLQQALGRTWLKRPEL
                     LERRGMSEEERALLEEFKSERAMRTGRTEDSQR"
     gene            247..591
                     /gene="rplS"
                     /locus_tag="VGE92_03835"
     CDS             247..591
                     /gene="rplS"
                     /locus_tag="VGE92_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEY1312979.1"
                     /translation="MNKIIQELNGERATRKLPEFNPGDTVVVQVKVVEGDRERVQAYE
                     GVVIAKKNRGLNSSFTVRKISHGEGVERVFQTFSPSISEVSVKRRGAVRRAKLYYLRD
                     LSGKAARIDEKI"
     gene            606..2471
                     /gene="sppA"
                     /locus_tag="VGE92_03840"
     CDS             606..2471
                     /gene="sppA"
                     /locus_tag="VGE92_03840"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEY1312980.1"
                     /translation="MAVGSARLGFFGLVWRILDGIRRALHLVLLLVIFGFILAALHTS
                     IPIVPHSAALVIAPEGELVEQLSNDAVRRAFGQASGGPAPETLLRDVLDAIAAAKSDE
                     RIKLIVLDLGYLNTSGLSKLQEIGGALRDFRAAGKRVVVAADSLDQTQYYLAAQAGEV
                     YLDPMGLVYLDGFSYYRMFLKDAIDKLAIDVNVFRAGTFKSFTDQFSRSDMAASEREE
                     SSAWLQALWSAYSQDVTRARSLPAGALNEFVNDEAASMAAVNGDAAKLALQRGLVTGL
                     KSRRQMADELKGLVGEDSDSHSFNSIAMNQYLTAVRSKHVLKSKSDSKVGVVVASGEI
                     LDGHQPPGTIGGESTADLLRQARYDKAVKAVVLRVDSPGGSMFASEQILREVQSLRKA
                     GKPVVVSMSSYAASGGYYISAAANQIFASATTLTGSIGVFSVIPTFQRTLEKLGVKVD
                     GIGTTPLAGNMRQDRALSPATRLILQSSVDHAYAEFLRRVGEGRKKPVEDVDKIAQGR
                     VWAGSDAQRIGLVDHLGGLKDATDAAAKLAELPADYDVDYIEPELSLREELLMQLRSQ
                     TVRVGQLAGIIPPRSDLERVLDPVLEQARVIARLNDPRGLYAYCWCREPRMMRRE"
     gene            complement(2493..3524)
                     /gene="tdh"
                     /locus_tag="VGE92_03845"
     CDS             complement(2493..3524)
                     /gene="tdh"
                     /locus_tag="VGE92_03845"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEY1312981.1"
                     /translation="MKALVKARAEPGIWLQDVPEPAVGHNDVLIKVHRTAICGTDMHI
                     YNWDAWAQKTVPVPMAVGHEYAGEIVELGSEVRGFHTGDRVSGEGHITCGHCRNCRAG
                     RRHLCRNTSGVGVNRAGAFAEYLTIPATNVFKLPNAIDDEIASILDPFGNATHTALAF
                     NIVGEDVLIAGAGPIGIMAVAIARHCGARHIVITDINDYRLDLAAKMGASKALNVARE
                     SLDDAMRDLGMEEGFDVGFEMSGNATALREMLRTMNHGGSISMLGIPPGETAIDWNQV
                     IFKGLVIKGIYGREMFETWYKMSSMLQSGLDVRPVITHRLGINDYREGFEIMNSGKSG
                     KITLDWASL"
     gene            complement(3542..4732)
                     /locus_tag="VGE92_03850"
     CDS             complement(3542..4732)
                     /locus_tag="VGE92_03850"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF005394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEY1312982.1"
                     /translation="MLKSFRERLSRDTDALEAAGLLKRERVIGGAQGPVVRLADGREM
                     INLCANNYLGLANHPAVLQAAQEALVRYGYGVASVRFICGTQTVHRELEQRLSAYLGA
                     EDTILYSSCFDANGGLFETLLDEQDAVISDALNHASIIDGIRLCKARRLRYANNDLNE
                     LEQALKDSQSSRVRLIATDGVFSMDGSIARLKEICDLADRYAALVMVDDSHATGFIGA
                     RGRGSHELRGVMGRIDILTGTFGKALGGASGGYVAARKEIVGWLRQRSRPYLFSNSVA
                     PPVAAATIRVLDLLEGSTDLRERLHANSRYFRDQMQGLGFDLIPGEHPIIPVMLGDAP
                     LAVKLAEQVQAQGVYVVAFSFPVVPHGKARIRTQMSAAHSREQLDRVIQAFGTAGREL
                     AIIH"
     gene            4818..5213
                     /locus_tag="VGE92_03855"
     CDS             4818..5213
                     /locus_tag="VGE92_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEY1312983.1"
                     /translation="MDEKIDNQRLDKWLWHCRFYKTRSLASAAINGGKVHLNAARVKP
                     AHKIRAGDRVSLSLQGIVAEFEVLGLPVRRGPAAVAQAQFIESPASVARREKLREQQR
                     LAQVSRPRPEARPDKRDRRRLMRLQRDQT"
     gene            complement(5229..5654)
                     /locus_tag="VGE92_03860"
     CDS             complement(5229..5654)
                     /locus_tag="VGE92_03860"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY1312984.1"
                     /translation="MSTPINVSFLPNLLGYNLRRAQIALWKDFIRTVGATRSGIFSLL
                     VLVEANPGIAQIDLANQLMIDKASIVGLIHDLQRRKWAERHQSSVDRRRQGIFLTAEG
                     RRALKKLREDMLEHEARFTRMYTAEELGQLLQLLRRMHT"
     gene            complement(5768..6283)
                     /locus_tag="VGE92_03865"
     CDS             complement(5768..6283)
                     /locus_tag="VGE92_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY1312985.1"
                     /translation="MGTRRLTLLRHGKAQSIDSCAEDFERALTHRGIIEAREMATRIA
                     YRDLAPDLILVSPAERAWATAEIIAEACELEDKQVQCARELYLATPETTWRLLAKRDA
                     ALSHIMICGHNPGLSQIASRFGPKPQRRDLATAGIATALWRNAHWATLQPESADSCEL
                     DDPESMADLWA"
     gene            complement(6265..6579)
                     /locus_tag="VGE92_03870"
     CDS             complement(6265..6579)
                     /locus_tag="VGE92_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEY1312986.1"
                     /translation="MAAKWIDAGPAAELAEGQTLSIPAGRRMIAVARSEGAYFAIEDI
                     CTHDGAALTGGAIEGAQIICPRHGARFCLRTGQALTPPAYEPVRVFETKIEGGHLWVR
                     AD"
     gene            complement(6596..7147)
                     /gene="sufT"
                     /locus_tag="VGE92_03875"
     CDS             complement(6596..7147)
                     /gene="sufT"
                     /locus_tag="VGE92_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HEY1312987.1"
                     /translation="MHSYENEPIVVQREVKAVAIPAGVEVNLRLGQVGYITQALGGSF
                     TVYIDGNLFRIAGQDADAIGKTAATAPEVPPGASEEDIKNIVWKQLKTCYDPEIPVNI
                     VDLGLIYECDVSKNEDSTRTVDIKMTLTAPGCGMGEVLVQDVRDKVQIVPTVARANVE
                     LVFDPPWNQTMMSDEARLQTGMM"
     gene            complement(7159..7614)
                     /locus_tag="VGE92_03880"
     CDS             complement(7159..7614)
                     /locus_tag="VGE92_03880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEY1312988.1"
                     /translation="MELDDLYRDVILDHNRKPRNFGVLEPADASVEGFNPMCGDRLTL
                     RLKLNAGKISDIRFEGHGCAISTASASLMTEAVKGRTRDEALRLFDRVHRLLTDDAAP
                     AAEELGKLAALSGVREYPARVKCASLCWHTPAAALNSTDRTPLPAVSTE"
     gene            complement(7620..8870)
                     /locus_tag="VGE92_03885"
     CDS             complement(7620..8870)
                     /locus_tag="VGE92_03885"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEY1312989.1"
                     /translation="MNNIALANRSSEFDVHGLRRQFPVLARMVRGKPLAYLDNGASAQ
                     RPLAVIEAVEDYERHHHANIHRGVHTLSQEATALYESSRDRLVEFINARSRSEIIFVR
                     GTTEAINLVAQSYARPVLKPGDEILITHLEHHANIVPWQMVCEQTGARLVVAPIDAHG
                     EVHVEAVLALLSPRTRLFACAHVSNALGTVLPVRRLIAAAKARGIVTLIDGAQAVPHM
                     HVDVQDLDCDFYAFSGHKMFGPTGVGVLYGREQLLERMPPWQGGGEMILAVTFAKTTY
                     NGLPNKFEAGTPNISGGIGLGAAVDFLRGLDRNAAHAHETALLAHATAVLSKIAQLRI
                     VGTAADKASLVSFTVDGVHPHDLGTILDEDGIAIRTGHHCAMPVMDFFKLPATARASF
                     AFYNTFEEIDRLAAGVERARRLFA"
     gene            complement(8867..10177)
                     /gene="sufD"
                     /locus_tag="VGE92_03890"
     CDS             complement(8867..10177)
                     /gene="sufD"
                     /locus_tag="VGE92_03890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEY1312990.1"
                     /translation="MNPSPAVRSFTAQWQARSADALSAVRDEAMQRFLKLGVPTTRDE
                     TWRYTNLRSLAAQSYADAPRKSRAEIEPNASLSLLSKTDRAAALLVVNGYPIVPMSDG
                     LIDGMEINSLQELARIDPKLLLRFLEPLSDADQQRWNLLNTALFIDGLHLKITHRVSI
                     PLLILHVSTSEHANNIAFPRIIIEVASGASATIIEHHVAQGRHTPLNTSTTHIALERD
                     AQLEHYRVFATGADATHLDSLNIRQQQDSHCKQFTIALGGGLVRASLEAHLEKPGASL
                     DSYSLLVGHEDRHVDCVNIVTHAAPNTRSTQTARAIASDHSRVVFNSKVIVNAGAVNA
                     ESQQSCRGLLLSPTAEIDTRPQLEIHADEVKCAHGATTGRLDPDMLFYMLSRGLDRDA
                     AQSLLVFAFLSDVLTGMSVASARTAIENALIMQLPDSQTLQKFR"
     gene            complement(10174..10926)
                     /gene="sufC"
                     /locus_tag="VGE92_03895"
     CDS             complement(10174..10926)
                     /gene="sufC"
                     /locus_tag="VGE92_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEY1312991.1"
                     /translation="MLKITDLSARVGGKDILKGINLEVHAGEVHAIMGPNGSGKSTLA
                     QVLAGREDYEVTGGSVSFEGLDLLALPPEERARAGLFLGFQYPVEIPGVNNVYLLKAA
                     LNAKRKAAGEPEVDAYEFLTLIRQKMKFMQMSESFLTRGVNEGFSGGEKKRNEVLQML
                     VLEPKLAILDETDSGLDIDALKVVSAGVNSLRNPQRAVVLVTHYQRLLDYVVPDRVHV
                     LSGGRILKSGDSSLARELEQRGYDWVLETRVA"
     gene            complement(10944..>11783)
                     /gene="sufB"
                     /locus_tag="VGE92_03900"
     CDS             complement(10944..>11783)
                     /gene="sufB"
                     /locus_tag="VGE92_03900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEY1312992.1"
                     /translation="PMELSTYFRINAAKTGQFERTLIIAAEAASVSYLEGCTAPMRDE
                     NQLHAAVVELVALDNAQIKYSTVQNWYPGDENGLGGIYNFVTKRGECRGHNSKISWTQ
                     VETGSAITWKYPSCILKGDNSVGEFYSVAVANNYQQADTGTKMIHIGRNTKSTIVSKG
                     ISAGHAENTYRGLVKILPSAAGARNFTQCDSLLMGAKCAAHTFPYMEVKNPTATVEHE
                     ASTSKIGEDQLFYCLQRGISAEDAVNMIVSGFCKVVFKELPMEFAVEAQNLLSVSLEG
                     AVG"
BASE COUNT         2164 a         3639 c         3718 g         2262 t
ORIGIN      
        1 gcattacaca cggcccgctg ccatcgacgg cgcgacggtt ccagcggtat tgatgtcggg
       61 cgatcatgcg gcaatccgcc gctggcgatt gcagcaggcg ctgggccgga cctggctcaa
      121 gcggccggaa ctgctcgagc ggcgcggcat gagtgaggaa gagcgggcgt tgttggaaga
      181 attcaagagc gaacgtgcga tgcgcacggg gcgaaccgaa gacagtcaaa ggtgagggcg
      241 ataaatatga acaagatcat ccaggaattg aacggcgagc gcgcgacgcg caagctgccg
      301 gaattcaacc ccggcgatac ggtcgtggtg caggtcaagg tagtcgaggg cgatcgtgag
      361 cgcgttcagg cctacgaagg cgtggtcatc gccaagaaaa atcgtggtct aaattcctcg
      421 ttcacggtgc gcaagatctc gcacggcgaa ggcgtcgagc gcgtgttcca aaccttcagc
      481 ccctcgatca gcgaagtgag cgtcaagcgc cgcggtgccg tgcgccgcgc aaagctgtac
      541 tacttgcgtg atttgtcagg aaaggccgca cgcatcgacg agaagatctg accatcgagg
      601 cgtgaatggc tgtcgggtcc gctcgattgg gattttttgg actggtctgg cgaattctcg
      661 acgggatccg cagggccctg catctcgttc tgctgctcgt catcttcggg ttcatactcg
      721 cggccctgca cacctccatc cccatcgtgc cgcattcggc ggcgctggtg atcgcccccg
      781 agggcgagct cgtcgaacag ttgtcgaacg acgcggtacg ccgcgccttc ggccaggcct
      841 cgggcggccc ggcccctgag accttgctgc gcgacgtgct ggacgccatc gcggcggcaa
      901 aatccgacga gcgcatcaag ctcatcgtgc tcgatctggg ctatctgaat acgtcgggat
      961 tgtccaagct gcaggagatc ggcggcgcgc tgcgcgattt tcgcgcggcg ggcaagcgcg
     1021 tcgtggtggc ggcggattcg ctggatcaga ctcagtacta tctggcggca caagccggcg
     1081 aggtctacct cgatccgatg ggcttggtgt atctcgatgg cttcagctac taccgcatgt
     1141 ttctgaagga tgccatcgac aagctggcga tcgacgtcaa cgtgttccgc gcaggtacct
     1201 tcaagagctt caccgaccaa ttctcgcgca gcgacatggc agcgagcgag cgcgaagaga
     1261 gttcggcctg gctgcaggca ttgtggagcg cctatagcca ggatgtcacg cgcgcccgct
     1321 cgttgcccgc aggcgcgctc aatgaattcg tcaacgacga agcggcgtcg atggccgctg
     1381 tgaacggcga tgccgccaaa ttggcgctgc agcgcggcct ggtcaccggc ctcaagagcc
     1441 gccgacaaat ggccgatgag ctcaaagggt tggtgggcga ggactccgac agccattcct
     1501 tcaactccat cgccatgaat caatatctca ccgcggtgcg ttcgaaacat gtgttgaagt
     1561 ccaaatcgga ctccaaagtg ggcgtcgtgg tggcatcggg agagattctc gacgggcatc
     1621 aaccgcccgg cacgatcggc ggcgaaagca cggcggacct gttgcggcag gcgcgctacg
     1681 acaaagcggt gaaggccgtg gtgctgcgcg tcgacagtcc ggggggcagc atgttcgcct
     1741 cggagcagat cctgcgcgaa gtgcaatcgt tgcgcaaagc cggcaagccc gtcgtggttt
     1801 cgatgagcag ctatgcggcg tccggcggat attatatttc cgccgcagcc aaccagatat
     1861 tcgcgagcgc cacgaccttg acgggatcga tcggcgtgtt ctccgtcatt cccaccttcc
     1921 aacgcacgtt ggagaagctc ggcgtgaagg tcgacggcat cggcaccacg ccgctcgccg
     1981 gcaatatgcg ccaggaccgc gcgctctcgc cggcgacccg gctgattctg cagagttcgg
     2041 tcgaccatgc ctatgcggaa tttctgcgcc gcgtcggcga agggcgaaaa aaaccggtcg
     2101 aggacgtcga caagatcgct caaggccggg tctgggcggg cagcgacgcg cagcgtatcg
     2161 gtttggtgga ccatctcggc ggcctcaagg atgcgaccga tgcggcggcg aaactcgccg
     2221 aattgcctgc cgactacgac gtcgattaca tcgagccgga attgagcttg cgcgaagaac
     2281 tcttgatgca gctgcgttcg cagacggtgc gcgtggggca gctggccgga atcatcccgc
     2341 cgcgcagcga cctcgagcgg gtgctcgatc ccgtgctcga gcaggcgcgc gtcatcgcga
     2401 gactcaacga cccgcgcggg ctatacgcat attgctggtg ccgggagccg cgaatgatgc
     2461 gccgcgagtg acgccgcggc agccaccgcc cgttacaggc tggcccaatc cagagtgatc
     2521 ttgccggact tgcccgagtt catgatttca aagccttcgc ggtagtcgtt gatccccaat
     2581 cgatgcgtga tcacggggcg cacatcgagc ccgctctgca gcatgctcga cattttgtac
     2641 caggtctcga acatctcgcg accgtagatg cctttgatca ccaacccctt gaagatcacc
     2701 tggttccagt cgatggcggt ttcgccgggc ggaatgccga gcatggaaat acttccgcca
     2761 tgattcatgg tgcgcagcat ctcgcgcagc gccgtggcgt tgccggacat ctcgaaaccg
     2821 acgtcgaatc cttcctccat gcccaagtcg cgcatggcgt cgtcgagcga ttcgcgcgcg
     2881 acgttgagcg ccttggaggc gcccattttt gccgcaaggt ccaaccgata gtcgttgata
     2941 tccgtgatca cgatgtgccg agcaccgcaa tgccgggcga tcgccaccgc catgatgccg
     3001 atcgggccgg ccccggcgat cagcacatcc tcgccgacga tattgaaggc gagcgccgtg
     3061 tgcgtcgcat tgccgaacgg atccaagatc gatgcaatct catcgtcgat ggcgttcggt
     3121 aatttgaaaa cattggtcgc cggaatcgtc agatactcgg caaatgcacc ggcgcggttg
     3181 acgcccacgc ccgaggtatt gcggcatagg tggcgacggc cggcacggca attgcggcaa
     3241 tggccgcagg tgatgtggcc ttctccggaa acgcggtcgc cggtatgaaa gccgcgcacc
     3301 tcgctgccca attcgacgat ttcccccgca tattcatgac cgaccgccat gggcaccggt
     3361 acggttttct gcgcccaggc atcccagttg taaatgtgca tgtcggtgcc gcaaatcgcg
     3421 gtgcggtgca ccttgatcaa aacgtcattg tggccgaccg ccggttccgg gacatcctgc
     3481 agccagatgc cgggttccgc gcgcgccttg accagagctt tcatttatta cctatccgcc
     3541 tctagtgaat gatggcgagt tcgcgacccg ccgtgccaaa tgcctgaata acccgatcca
     3601 gttgttcgcg ggagtgtgca gcgctcattt gggtgcgaat tcgcgctttt ccatgcggca
     3661 ccaccggaaa ggagaaagcg acgacgtaga cgccctgcgc ctggacctgc tccgcgagtt
     3721 tgaccgccaa cggcgcatcg cccagcatga ccgggatgat gggatgttct ccggggatca
     3781 aatcgaaccc caatccctgc atctggtcgc ggaagtaacg ggaattggcg tgcagccgct
     3841 cgcgcaggtc ggtcgaccct tccaacagat cgagcacccg gatcgtggcc gccgccaccg
     3901 gcggcgcgac gctgttcgag aacagatacg gccgcgaacg ctggcgcagc cagccgacga
     3961 tttccttgcg cgcggccaca taaccgccac tcgcgccgcc caaggctttg ccgaaggtac
     4021 cggtcagaat atcgatacgg cccatgacgc cgcgcagctc gtgcgagccg cggccgcgcg
     4081 cgccgatgaa accggtcgca tgcgaatcat cgaccatgac gagcgcagcg tagcggtccg
     4141 ccaagtcgca aatttccttc aatctcgcga tcgagccgtc catggagaag acgccgtcgg
     4201 ttgcgatcag gcgcactcgc gaggattgcg aatctttgag cgcctgttcg agttcattga
     4261 gatcattgtt ggcgtagcgc aggcgacgcg ccttgcaaag acgaatgccg tcgatgatgc
     4321 tggcgtgatt caaggcatcg ctgataacgg cgtcctgctc atcgagcagc gtctcgaaca
     4381 atccgccgtt cgcatcgaag caggaagaat acagaatcgt atcttccgcg ccgaggtagg
     4441 cgctcaggcg ctgctccaat tcgcgatgca cggtttgcgt gccgcagatg aagcgcaccg
     4501 aggcgacgcc atagccgtag cgcacgagcg cctcttgcgc ggcttgcaaa accgcggggt
     4561 gattcgccag accgagataa ttgttggcgc acagattgat catctcgcgc ccatcggcga
     4621 ggcgcaccac gggtccttgc gctccgccga tgacgcgctc gcgcttcaac agaccggccg
     4681 cctcgagtgc gtccgtgtcg cgcgacaggc gctcacgaaa acttttcagc acgggcaact
     4741 tggctggcaa atcgggcgtt tcattccggc tattataacg acttcgcgtg gctacccgcg
     4801 cggaatgcac aggggttatg gacgagaaaa tcgataatca gcgcctggat aagtggcttt
     4861 ggcactgccg tttttacaaa acgcgcagcc tcgcaagcgc cgcgatcaac ggcgggaaag
     4921 tgcatttgaa cgccgcccgg gtcaaaccgg cgcacaagat ccgcgccggc gatcgggtga
     4981 gcctctcgct gcagggtatc gtggctgagt tcgaagtgct ggggctgccg gtgcgccgcg
     5041 ggccggccgc cgtggctcag gcgcagttca tcgagtcgcc ggcgagtgtc gcgcgccgcg
     5101 aaaagctgcg cgagcaacag cgtctggcgc aggtttccag gccgcggccc gaggcgcgcc
     5161 cggacaagcg cgatcgacgg cgcctgatgc gcttgcagcg cgatcagacc tagcaggttc
     5221 gaacccgctc aagtgtgcat gcggcgcagc agctgcagca gctggccgag ttcttcggcc
     5281 gtgtacatgc gcgtaaatcg cgcctcgtgt tccaacatgt cctcgcgcag ctttttcaag
     5341 gcgcggcgcc cctcggccgt cagaaatata ccctggcgtc gccgatcgac gctcgattga
     5401 tgccgctccg cccatttgcg ccgctgcaga tcgtggatca ggccgacgat cgaggccttg
     5461 tcgatcatca gttgattggc gagatcgatc tgtgcgatgc ccggattcgc ctcgaccaac
     5521 accagcagac tgaaaatgcc ggagcgggtt gcgcccacgg tgcggataaa atccttccac
     5581 aatgcaattt gcgcgcgccg caaattgtag ccaagcagat tgggcaaaaa gctgacattg
     5641 atcggagtgg acacggctgg cttgcggcca acgcctttcg cttcgccggc ggcacgcacg
     5701 ggtttgtgat gagatcgcaa tcgggccggc atgcacgcct cgctggtcaa ggccggtaat
     5761 tatgcattca agcccataga tccgccatgc tctccggatc gtcgagttcg cagctgtcgg
     5821 cggattcggg ctgcaacgtc gcccaatgcg cgttgcgcca cagcgccgtg gcgatgccgg
     5881 cggttgccag atctcggcgc tgcggtttcg ggccgaagcg gctggcgatc tggctcagac
     5941 ccgggttatg cccgcagatc atgatgtggc tcaaggccgc gtcgcgcttc gcgagcaggc
     6001 gccaggtggt ctccggcgtt gccaagtaca attcacgcgc gcattgcact tgcttgtcct
     6061 cgagctcgca ggcttcggcg atgatttcgg cggtggccca ggcgcgttcc gccgggctca
     6121 ccaggatcaa atcgggcgcg agatcgcggt acgcgatgcg cgtggccatt tcgcgcgctt
     6181 cgatgatgcc gcggtgggtc agcgcgcgct cgaaatcctc ggcgcaggag tcgatggatt
     6241 gggccttccc gtgccgcaac agcgtcagtc tgcgcgtacc cacaaatgac caccttcaat
     6301 tttcgtttcg aagacgcgca ccggctcgta tgcgggcgga gtcaatgctt gtccggttct
     6361 caaacaaaag cgagcaccgt ggcgcgggca aataatttgc gctccctcga tggcacctcc
     6421 cgtcaaggcg gcaccatcgt gggtgcagat gtcctcgatg gcgaaatatg ccccctcact
     6481 gcgtgccacg gcaatcattc ggcgtcccgc cgggatggag agtgtttggc cctccgcgag
     6541 ttcagcagcg ggaccggcgt cgatccattt agcggccatg tccggtcatc cgctgctaca
     6601 tcatgccggt ttgcaagcgc gcttcatccg acatcatggt ctggttccat ggcgggtcga
     6661 acacgagttc gacgttcgcg cgcgcgacgg tcgggactat ttgcaccttg tcgcgcacgt
     6721 cctgcaccaa cacttcgccc attccgcagc ccggtgcggt caacgtcatt ttgatgtcga
     6781 cggtgcgcgt ggaatcctcg ttcttgctga catcgcattc gtagatcaat ccgaggtcga
     6841 cgatattgac cgggatttcc gggtcatagc aagtctttag ttgcttccag acgatgttct
     6901 tgatgtcctc ttccgaggcc cccggcggca cttcgggcgc ggtcgcggcg gtcttgccga
     6961 tggcatccgc gtcctggccg gcgatgcgga atagattgcc gtcgatgtac acggtgaaac
     7021 tgccgccgag cgcctgggtg atgtagccca cctgtcccaa tctgaggttg acttcgacgc
     7081 cggccggaat ggccaccgct ttgacctcgc gctgcacgac gatcggttcg ttttcgtagg
     7141 aatgcatcgt ttaaccgctc attcggtgga caccgcgggc agcggcgttc gatccgtgga
     7201 gttgagcgcg gcggcggggg tatgccagca caggctcgca cacttgacgc gtgcggggta
     7261 ttcgcgcacg cccgagagcg ccgcgagttt gcccagttcc tccgcggcag gcgcggcatc
     7321 atcggtgagc aagcggtgaa cgcgatcgaa cagacgcagc gcttcgtcgc gggtgcgccc
     7381 cttgaccgct tcggtcatca gcgaggccga cgccgtggag atcgcacatc cgtgtccttc
     7441 gaagcggatg tcgctgatct tgcccgcgtt caatttgagg cgcagcgtca ggcgatcgcc
     7501 gcacattggg ttgaaccctt cgacgctggc gtcggccggt tccaagacgc cgaaattgcg
     7561 cggcttgcgg ttgtggtcga gaatcacgtc tcgatacagg tcgtcgagtt ccatggcgat
     7621 caggcgaata gcctgcgcgc acgctcgacg ccggccgcaa gccggtcgat ttcctcgaac
     7681 gtattgtaaa aggcgaacga ggcgcgcgcc gtggccggca gcttgaagaa gtccatgacc
     7741 ggcattgcac agtgatggcc ggtgcggatg gcgatcccat cctcgtcgag aatcgttccc
     7801 agatcgtggg gatgcacgcc gtcgaccgtg aaagacacca ggctcgcctt gtcggctgcg
     7861 gtgccgacga tgcgcagttg cgcaatcttg ctcaacaccg cggtcgcatg cgcgagcagg
     7921 gcggtttcat gcgcatgcgc ggcgttgcgg tccaatccgc gcagaaagtc gaccgcggcg
     7981 ccgaggccga tgccgccgga gatattggga gtgccagcct cgaacttatt cggcaaaccg
     8041 ttgtaggtgg tcttggcaaa ggtcaccgcc aatatcatct cgcctccgcc ctgccatggc
     8101 ggcatgcgtt cgagcagctg ctcgcgtcca tacagaaccc cgacgccggt cggaccgaac
     8161 atcttgtgcc ccgagaaggc atagaaatcg cagtccagat cctgcacatc gacgtgcatg
     8221 tgcggcacgg cttgtgcgcc gtcgatcagc gtgacgatgc cgcgcgcctt ggcggcggcg
     8281 atcaagcggc gcaccggcag gaccgtgccc agggcattcg agacgtgggc gcacgcaaac
     8341 aagcgcgtgc gcgggctcag caacgcgagc accgcctcca catggacctc gccgtgcgca
     8401 tcgatgggcg cgaccacgag tcgcgcaccc gtctgctcgc aaaccatctg ccagggcacg
     8461 atgttcgcgt ggtgttccaa gtgggtaatg agtatttcat cgcccggttt gagcaccggc
     8521 cgcgcgtagc tctgcgcgac cagattgatt gcctcggtgg tcccgcgcac gaaaatgatc
     8581 tcactgcgcg agcgggcgtt gatgaactcg accagccggt cgcgcgagct ctcatatagc
     8641 gccgtcgctt cctgactcaa cgtgtgcaca ccgcggtgga tgttggcgtg atgatggcgt
     8701 tcgtagtcct cgaccgcctc gatcaccgcc agcggacgct gcgcggaagc gccattgtcc
     8761 agataagcca gcggtttgcc gcgcaccatg cgcgccagca ccggaaattg ccggcgcagg
     8821 ccgtgcacgt cgaattccga gctgcgattt gccagagcta tgttgttcat ctgaatttct
     8881 gcagggtttg cgaatcgggt agctgcatga tcagcgcgtt ctcgatggcg gtgcgcgccg
     8941 aagccaccga catgccggtg agcacgtcgg acaggaaagc gaataccaag aggctttgcg
     9001 ccgcgtcgcg gtcgagaccg cgcgacagca tgtaaaagag catgtccggg tcgagccggc
     9061 ccgtggtcgc gccgtgcgcg catttgacct cgtcggcatg gatttccaat tgcgggcggg
     9121 tgtcgatttc ggccgttggc gacagcagca agccgcggca cgactgctgc gactcggcat
     9181 tgaccgctcc ggcattgacg atcactttac tattgaacac gacgcggctg tggtcgctcg
     9241 cgatggcgcg cgcggtctgg gtgctgcggg tgttgggcgc ggcatgggtc acgatattga
     9301 cgcagtcgac gtgccggtcc tcgtgtccga ccagcaggga atagctgtcg agcgaggcgc
     9361 cgggcttctc gaggtgcgcc tcgagagagg cgcgcaccaa gccgccgccc agcgcgatcg
     9421 tgaactgctt gcagtggctg tcctgctgct ggcggatgtt cagggaatcc aaatgcgtgg
     9481 cgtcggcgcc ggttgcgaac acacggtagt gctcgagctg ggcgtcgcgc tcgagcgcga
     9541 tgtgcgtggt ggacgtgttt agcggggtgt gccgaccttg cgcgacgtgg tgctcgatga
     9601 tggtagcgct cgctcccgac gcgacctcga tgatgatgcg cgggaaagcg atgttgttgg
     9661 cgtgctcgct ggtcgagacg tgcagaatca gcagcggaat cgacacgcgg tgcgtaatct
     9721 tgaggtgcag cccgtcgatg aacagcgcgg tattgagcaa gttccagcgc tgctggtcgg
     9781 cgtccgacag gggctccagg aagcgcagca acagcttcgg atcgattcga gccagctctt
     9841 gtaagctatt gatttccatc ccatctatca ggccgtccga catcggcaca atgggatagc
     9901 cgttgaccac gagcagcgcc gccgcgcgat cggttttgct gagcagcgac aacgaggcgt
     9961 tgggctcgat ctccgcccgg gacttgcgcg gcgcatccgc atagctctgc gcggccaaag
    10021 agcgcaggtt cgtgtagcgc caggtctcat cgcgcgtcgt cggcacaccc agcttgagaa
    10081 accgctgcat ggcttcatca cgtaccgccg agagcgcatc ggcggatcgt gcctgccatt
    10141 gcgccgtgaa ggagcgaact gccggcgacg gattcatgcg actcgggtct ccagcaccca
    10201 atcatagccg cgttgttcga gttctcgggc gagcgagcta tctccggatt tcagaatccg
    10261 gccgcccgac agcacgtgca cccggtccgg cacgacatag tcgagcaaac gctggtagtg
    10321 agtcacgagc accacggcgc gctgcggatt gcgcagcgag ttcacgccgg cggacaccac
    10381 ttttagcgca tcgatgtcga gcccggaatc cgtctcgtcc aaaatcgcaa gcttgggctc
    10441 caagaccagc atctgcagaa cctcattgcg cttcttctcg ccgccggaga aaccctcgtt
    10501 gacaccgcgc gtcagaaacg actcgctcat ctgcatgaac ttcattttct ggcggatgag
    10561 agtcaggaat tcataggcgt cgacttcggg ttcgccggcc gccttgcgct tggcattgag
    10621 cgccgccttc agcaggtaga cgttgttgac gccgggaatt tccaccggat attgaaagcc
    10681 gagaaacaag ccggcgcgcg cccgctcctc gggcggcaac gccagcaggt cgaggccctc
    10741 gaaactcacc gaaccgcccg tcacttcgta atcctcgcgg ccggccaaca cctgcgcgag
    10801 cgtgctcttg ccggatccat tcgggcccat gatggcgtgc acctcgccgg catggacctc
    10861 gagattgatg cccttcagta tgtccttgcc gcccacgcgg gcgctcaagt cggtgatttt
    10921 taacacgctc ttccccgttg ccgctagccg acggcgcctt ccaaactcac gctcaaaaga
    10981 ttctgcgctt cgacggcgaa ttccatcggc agttccttga acaccacttt gcaaaacccg
    11041 ctgacgatca tgttgaccgc gtcttccgcc gagatgccgc gctgcaagca ataaaagagc
    11101 tggtcctcgc cgatcttcga ggtgctggcc tcgtgctcga ccgtggcggt gggattcttg
    11161 acttccatat atggaaacgt gtgcgccgcg cacttcgcgc ccatgagcag cgaatcgcac
    11221 tgggtgaaat tgcgcgcccc cgcggcgctc ggcaggatct tcaccagtcc gcgataggta
    11281 ttctcggcat ggcctgccga gatgcctttc gacacgatcg tgctcttggt gttgcgcccg
    11341 atgtggatca ttttcgtgcc ggtgtcggcc tgctggtagt tattggcgac cgccacggaa
    11401 tagaattcgc ccaccgagtt gtcgcccttc aagatgcagc tcggatactt ccacgtgatg
    11461 gctgagccgg tctccacctg ggtccacgag attttcgagt tgtggccgcg gcattcgccg
    11521 cgcttggtga cgaagttgta aatcccgccc aagccgttct catcccccgg ataccagttc
    11581 tgaacggtcg agtacttgat ctgcgcgttg tcgagagcca ccagttccac gacggcggcg
    11641 tgcaattggt tctcatcgcg catcggcgcg gtgcagccct ccaggtagct gaccgacgca
    11701 gcctccgcgg cgatgatcag cgtgcgctcg aattgaccgg tcttggcggc gttgatgcga
    11761 aagtaggtcg acaactccat cgg
//
LOCUS       DASWZT010000141         5833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_49340, whole genome shotgun sequence.
ACCESSION   DASWZT010000141 DASWZT010000000
VERSION     DASWZT010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5833
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49340"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..1890
                     /locus_tag="VGE92_03905"
     CDS             274..1890
                     /locus_tag="VGE92_03905"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY1312993.1"
                     /translation="MKRNLLSAACAVCVALGLPAHAASDGGLLGSSDIKHVLLVSVDG
                     LHALDLSNYVASHPDSNLAQLSRHGVTYTNNATSSPSDSFPGLASLVTGGSPVTTGLW
                     YDDTYNRALSPPLMTDGLGNPGGNCPGTVGTNVAWDEAVDIDLTKLDGGGGLNPKYLV
                     RDPNNGCKTILPHQYLRVNTIFEVVKAAGGRTAWTDKHPSYEWTNGPSGKGVDDFWGP
                     EINSVPVALPQFSGCSPVPFADPTPDDGWTNSFDDIQCYDQLHVQAVINEIDGYNHDR
                     SAKVGVPTLFGTNFQSVSVGEKLAKDPVSGLKGGYTDVLGTPGQGLAGELEFIDRSIG
                     RFVQELKAQRLYDSTLIIISAKHGQSAIDVSKRRGIADGQPTATIGSADAFDISDDGS
                     LIWLTDPSLAPGVVANLSTPANQQALGIQEIFAGQSLRNKFNNPGADPRTPDIILKVN
                     TGVIFTGGSKLSEHGGFNEDDVHTALLVSLDGMQHAVVKSATSNQQVAATIVKALGID
                     PNELEAVRKEQIQVLPFLFSGKVDNKITGF"
     gene            complement(1944..2540)
                     /locus_tag="VGE92_03910"
     CDS             complement(1944..2540)
                     /locus_tag="VGE92_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1312994.1"
                     /translation="MNSRGRPWWMAPRFAPLLGASIGAAGGAIYWLGALIWPSSIAVI
                     LSVFATTLLSAAAAGVGASAQAASSGVVGVVFAILVKYDALLALSSASLPFAVPANVA
                     LGFIMIAGHAASRALLVSVSKPVSYADLGIALGMGFAPAVLIGIPGLAGVAAAIAARL
                     LLIGYLKRRRQGAADFDLTLRLTEACFYLGALATWAYV"
     gene            complement(2551..5397)
                     /gene="uvrA"
                     /locus_tag="VGE92_03915"
     CDS             complement(2551..5397)
                     /gene="uvrA"
                     /locus_tag="VGE92_03915"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004797750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY1312995.1"
                     /translation="MQNIRIRGARTHNLKNIDLDLPRDRLIVLTGLSGSGKSSLAFDT
                     IYAEGQRRYVESLSTYARQFLSMMEKPDVDTIEGLSPAISIEQKSTSHNPRSTVGTVT
                     EIYDYLRLLYARAGTPRCPDHDIDLAAQTVSQMVDAVLEHPEGTALMLLAPAVAERKG
                     EHAELIEELQAGGFVRARIDGKVVELDEAPKLDVRRKHTVEAIVDRFKVRPDLSQRLA
                     ESFETALRLGSGVARVAYMDDPQRAELVFSDKFACPICNYSLSELEPRLFSFNSPIGA
                     CPACDGLGTQDFFDPDKIVANPHLSLAGGAVRGWDRRNAYYFQLIQSLARHTKFDIEA
                     PFETLPQNIKDLVLFGSNDVEIEFKYLDGKGGTLRRKHTFEGIVKNLERRYNETESAT
                     VREELAKYRSSRPCPECLGTRLNRAARNVFVGGRSVPEVSSLSVDRALEFFGQLKLAG
                     WRGEIAVKIVKEIGDRLGFLGNVGLGYLTLNRSADTLSGGEAQRIRLASQIGSGLVGV
                     MYILDEPSIGLHQRDNQRLLDTLVNLRDLGNTVIVVEHDEDAIRQADHVVDLGPGAGI
                     HGGAIVAQGTAAEVAAHPDSITGQYLSGRRRIEVPPRRRAPDPSRMLRVLGATANNLK
                     KVTAEFPLGLLTCVTGVSGSGKSTLVNDTLFRAVATQMNHAAPGSAHFDRIEGLEHID
                     RVIEIDQSPIGRTPRSNPATYTGLFAPIREIFAAVPESRARGYEAGRFSFNVKGGRCE
                     ACQGDGVIKVEMHFLADVYVPCDVCRGKRYNRETLEIRFKGKNIQEVLEFTVEDALPF
                     FSAVPTVQPKLQTLLDVGLSYVKLGQSATTLSGGEAQRVKLAKELSKRATGRTLYILD
                     EPTTGLHFADIAQLLVVLQRLRDEGNTVIVIEHNLDVIKTADWVVDLGPEGGDGGGRI
                     VAVGTPEQIAANKVSYTGRYLRSKLTAPPVRKRA"
     gene            5560..>5833
                     /gene="ssb"
                     /locus_tag="VGE92_03920"
     CDS             5560..>5833
                     /gene="ssb"
                     /locus_tag="VGE92_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY1312996.1"
                     /translation="MARGVNKVILVGNLGADPETRSMPSGMTVTNIRIATSESWKDKA
                     SGAQQERTEWHNIALFGRLGEIAAEYLRKGSQVFVEGKLRTRKWQDK"
BASE COUNT         1126 a         1813 c         1854 g         1040 t
ORIGIN      
        1 atgccccagg agagcgaatc ggagtagacg agaatttgct gcaatgccta aggtctcccg
       61 gcgttctttt atgcttgcgg ggcaaaggtc gccgcatcga cggcagcgct cattatagac
      121 gactcgcatt cgggagaggt cgccgccgtc cggcgcatgc cgcctgcgcg cgcccgcttt
      181 cgcggctttg taacacaaag accgtaatgt gctgtgcacg ccgcgcagcc aaggggctcg
      241 cacagcggcg tccatctaac aaaaggagtc atcatgaagc gcaatctatt gagcgccgcg
      301 tgtgctgtct gtgtggcatt agggttgccc gcgcacgctg catcggacgg gggactgttg
      361 ggatccagcg acatcaagca cgtgctattg gtcagcgtgg acggtctgca cgcgctcgac
      421 ttgtccaatt acgtggcgtc gcatccggac tccaatttgg ctcaattgag ccgccatggc
      481 gtgacgtata cgaataatgc cacctcgtcg ccttcggact cgtttcccgg actcgcctcg
      541 ctggtcaccg gcggatcgcc ggtcaccacc ggcttgtggt acgacgacac gtacaaccgg
      601 gcgctgtccc cgccgctgat gaccgacggc ctcggaaatc ccggcggcaa ctgtcccgga
      661 acggtcggta ccaacgtcgc ctgggacgag gcggtcgaca tcgatctgac gaagctcgac
      721 ggcggcggcg gactcaatcc gaagtacttg gttcgcgacc cgaacaacgg ctgcaagacc
      781 attctgccgc atcagtacct gcgggttaat acgattttcg aagtggtgaa ggcagcgggc
      841 ggccgaaccg cgtggaccga caaacacccg tcgtacgaat ggaccaacgg accgagcggc
      901 aagggtgtgg atgatttctg gggcccggag atcaattcgg tgccggtggc tctgccgcaa
      961 ttctcgggtt gctcgccggt cccctttgcg gatccgacgc ccgacgatgg ctggaccaac
     1021 agcttcgatg acattcagtg ctacgaccaa ttgcacgtgc aagcggtgat caatgagatc
     1081 gacggctaca atcacgatcg cagcgcgaag gtgggagtgc ccacgttgtt cggcaccaac
     1141 ttccagtcgg tgagcgtcgg cgagaagttg gcaaaggatc ccgtgagcgg actcaagggc
     1201 ggctacacgg atgtgctcgg tacgccgggg cagggacttg ccggggagct cgaattcatc
     1261 gatcggtcga tcggccgctt cgtgcaggag ctcaaagcgc aacgcctcta cgactcgaca
     1321 ttgatcatca tcagcgccaa gcacgggcaa tcggccatcg atgtcagcaa gcgccgcggc
     1381 atcgccgatg gtcaaccgac ggcgaccatc gggagcgcgg acgcattcga catctccgac
     1441 gacggttcgc tcatctggct caccgatccg tcgttggcac cgggggtggt tgccaacctc
     1501 agtacgcccg cgaatcaaca ggcgctcggc atccaggaga tttttgcggg ccagtcgctg
     1561 cgcaataaat tcaacaaccc cggcgccgat ccgcgcacgc ccgatatcat cctcaaggtc
     1621 aacaccggcg tgatcttcac cggcggctcg aaattgtcgg agcacggcgg tttcaacgag
     1681 gacgatgtgc ataccgcgct gttggtctcg ctcgacggta tgcaacatgc cgtcgtcaaa
     1741 agtgcgacga gcaatcagca ggtcgctgcc acgatcgtca aggccctcgg catcgacccc
     1801 aacgaactcg aggcggttcg caaggagcag atccaagtgt tgccgtttct gttcagcggc
     1861 aaggtcgata acaaaataac cgggttctaa gaactcgatg cgggagcgag gcgcaccggc
     1921 cgaccggtgc gcctcccaag cgactacacg taggcccagg tggcgagcgc gccgagatag
     1981 aagcacgctt cggtgagccg cagggtcagg tcgaagtctg cggcgccctg acgccggcgc
     2041 ttgagatagc cgatcaacag caggcgcgcg gcgatggccg cggccactcc ggcgagaccc
     2101 ggtatgccga tcagcacggc gggcgcgaac cccatgccga gcgcgatgcc gaggtcggcg
     2161 tacgagaccg gttttgaaac ggacacgagc agcgcgcggc tggctgcgtg acccgcaatc
     2221 atgatgaaac cgagagctac attggcaggc actgcgaagg gcaggctggc ggatgacaac
     2281 gccagcagcg cgtcgtactt cacgagaatc gcaaaaacaa cgccgacgac gcctgaactc
     2341 gcggcctgcg cgctcgcgcc caccccggcg gccgcggcgg acaggagggt cgtcgcaaac
     2401 acggacaaaa tgacggcgat gctgctcggc cagatcagcg caccaagcca gtagatggcg
     2461 ccgcccgcgg caccgatcga ggcgccgagc agcggcgcga aacgcggcgc catccaccag
     2521 gggcgcccgc ggctgttcat gcgtggctgt tcaagcgcgt tttcgcaccg gcggcgccgt
     2581 caatttggag cgcaaatagc gtccggtgta cgaaaccttg ttcgcggcga tctgttccgg
     2641 cgtgcccact gccactattc ggccgccgcc gtcgccgcct tcgggtccca agtccaccac
     2701 ccagtccgcc gttttgatga cgtccagatt gtgctcgatg acgatgacgg tgttgccttc
     2761 gtcgcgcaga cgctgcagca ccacgaggag ctgggcgata tcggcgaaat gcagtccggt
     2821 cgtcggttca tcgaggatgt acagcgtgcg cccggtcgcc cgcttggaaa gctccttggc
     2881 cagcttgact cgttgggctt cgccacccga gagggtggtg gcgctctgtc ccaatttgac
     2941 gtaggaaaga cccacgtcga gcagggtttg cagcttcggc tgcaccgtcg gcacggcgga
     3001 aaaaaacggc agcgcgtcct ccaccgtgaa ttcgagcacc tcttggatat tcttgccctt
     3061 gaagcgaatc tccaaggtct cacggttgta gcgcttgccc cggcacacat cgcaaggcac
     3121 atacacgtcg gcgagaaaat gcatctccac cttgatcacg ccgtcgccct ggcaggcttc
     3181 gcagcggccg cccttgacgt tgaagctgaa tcgccccgcc tcgtagccac gcgctcggga
     3241 ttcggggacg gcggcgaaga tctcgcggat cggcgcgaac aatcccgtgt aggtggccgg
     3301 attcgagcgc ggcgtgcggc cgatcggact ttggtcgatt tcgatcaccc ggtcaatgtg
     3361 ttcgagccct tcgatgcgat cgaaatgcgc gctgccgggt gcggcgtggt tcatttgcgt
     3421 cgcgaccgca cgaaacagcg tgtcgttgac cagggtcgac ttgccggaac cggagacccc
     3481 ggtgacgcag gtcagcaagc ccagcggaaa ttccgccgtg accttcttta aattgttggc
     3541 cgtcgcgccc agtacccgca gcatgcgcga cggatccggc gcgcggcgcc gcggcggcac
     3601 ttcgatgcgg cggcggccgc tcaaatactg cccggtgatc gaatcgggat gcgcggcgac
     3661 ctccgcggcc gtgccttgcg cgacgatggc gccgccgtgg atgccggcgc cgggacccaa
     3721 gtcgacgaca tgatcggcct ggcgaattgc atcttcatcg tgttcgacca caatcacggt
     3781 gttgccgaga tcgcgcagat tcacgagcgt gtcgagcagc cgctggttgt cgcgctgatg
     3841 cagaccgatc gacggctcgt cgagaatgta catcacgccg acgagaccgg aaccgatttg
     3901 actggccagc cgaatgcgct gcgcctcgcc gccggacaag gtatcggcgc tgcgattcaa
     3961 ggtcagatag ccgagtccga cattgccgag aaaccccagc cggtcgccga tctccttgac
     4021 gatttttacg gcgatctcgc cgcgccagcc cgcaagcttc agctggccga agaactcgag
     4081 cgcgcgatcg accgacagcg acgagacttc cggaacactc cggccgccga cgaagacgtt
     4141 gcgcgccgcg cggttgagcc gcgtcccgag gcattcggga catgggcgcg atgagcgata
     4201 tttcgcgagc tcctcgcgta ccgtcgccga ctcggtttcg ttgtaacggc gctcgagatt
     4261 cttgacgatg ccttcgaagg tgtgtttgcg cctgagcgtg ccgcccttgc cgtccaagta
     4321 cttgaactcg atctcgacgt cgttgctgcc gaacaggacc aaatccttta tattttgcgg
     4381 cagggtctcg aagggcgcct cgatatcgaa cttggtatgc cgagccagcg attgaatcag
     4441 ctggaagtag taggcgttgc gccgatccca gccgcgcacc gcgccgccgg ccagcgacaa
     4501 gtgcggattc gcgacgatct tgtccgggtc gaaaaaatcc tgtgtgccga ggccgtcgca
     4561 cgccgggcag gcgccgatcg ggctgttgaa tgaaaacagc cgcggctcga gctcgctcaa
     4621 cgagtagttg caaatcgggc aggcaaactt gtcggagaaa accagttcgg cccgctgcgg
     4681 gtcgtccatg taggcgactc tcgccacccc agacccaagc cgcagcgccg tttcgaacga
     4741 ctcggccagg cgttggctca aatcgggccg aactttgaag cgatcgacga tggcttcgac
     4801 cgtgtgcttg cggcggacat cgagtttcgg cgcctcgtcg agctcgacga ccttgccgtc
     4861 gatgcgggcg cgcacgaacc cacccgcctg cagctcctcg atcagctcgg cgtgttcgcc
     4921 cttgcgctcg gcgacggcgg gtgccagcaa catgagggcg gtgccttccg gatgttcgag
     4981 aacggcgtcg accatctggc tcacggtctg cgcggccaag tcaatgtcgt ggtccgggca
     5041 gcgcggcgtg ccggcgcgtg catacagcag ccgcagatag tcgtaaatct cggtcacggt
     5101 gccgacggtc gagcgcgggt tgtgcgaggt cgatttctgc tcgatcgaga tcgccggcga
     5161 caggccttcg atggtgtcga cgtccggctt ctccatcatc gacaaaaatt gtcttgcgta
     5221 cgtcgacaag gattcgacat agcgccgctg accctcggcg tagatggtgt cgaaggcgag
     5281 cgaggacttg ccggagcccg acagcccggt caagacgatc aatcggtccc gcggcaggtc
     5341 gagatcgatg ttcttcaggt tatgagtccg ggccccccgg atgcggatat tttgcatgta
     5401 accgactact atacccgcgg cgtcaagcgc gggtaaaacg gccccgaagc tcgattccgg
     5461 cgggtggatt tctgcacaag cgcagtgttc aagccccgta ttggggtggg ttcctgcgtg
     5521 tacaataccg gccattcaat cgtttcccag gggtcagaca tggcgcgcgg cgtaaacaag
     5581 gtcattttgg tgggtaatct cggtgcggac ccggaaaccc gctccatgcc gagcggaatg
     5641 acggtgacca acatccggat cgccaccagc gagtcgtgga aggacaaggc ctccggcgcg
     5701 cagcaggagc gcaccgaatg gcacaacatc gcgctgttcg gccggttagg cgaaatcgcc
     5761 gccgagtacc tgcgcaaggg gtctcaggta ttcgtcgaag gcaaattgcg aacccgcaag
     5821 tggcaggata agc
//
LOCUS       DASWZT010000142         4654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_73616, whole genome shotgun sequence.
ACCESSION   DASWZT010000142 DASWZT010000000
VERSION     DASWZT010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73616"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2281)
                     /locus_tag="VGE92_03925"
     CDS             complement(<1..2281)
                     /locus_tag="VGE92_03925"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1312997.1"
                     /translation="MKSHARVVVIGGGVVGVSTLYHLALKGWSDVVLLERAELTAGST
                     WHAAGLLPLFNMSYTVGQLHKYSVDLYKRLPAETGQDVSFHVTGNLRLATCRDRMDEY
                     QKYCGTANTIGVPFQVIGPQQVRDLWPLVELGDGAGTPKIIGALYHPDDGHIAPADLT
                     MALRKGARSRGAEINEQTEVTAVKRTASGEWHLTTNRGDIVAEHVVCATGNYARQTGR
                     LFGLNVPSIPVEHQYIVYDESPELKAYRQGGGRELAVLRESDQSYYLREERLGWILGP
                     YEAGAPARFADGVPDWFGKSLFEGDLERLLPHVEAAQRRVPSLATCGIKDIVNGPIAY
                     TPDGSPLIGPAWGARNLWLNEGHSFGITAAGGSGWQLAEWIVEGEPGIDMMAVDPRRY
                     GPYTSKRYVVQKNEETYRNVFVIHYPDEERADARPAKTSPVYDKLQRMGAVFGQRYGW
                     ERANWFAPPGVPAKDHWSFRRSNYFQHVGNEARRLREHVGIIDLTPFTKHEVSGPGAE
                     SWLDGLVANKVPTKIGRIALCHALTRRGGIRSEFTITKIADDHFYVVSAGAAERYDSD
                     YLQQMLPQDGSVSLRNITMSRGVFVLAGPRSREVLAKLTDAPLDNAAFPWLTGQVAEV
                     GLATDVYLLRVNFVGALGWELHFPIEYAHHLFDSLFAAGAEFSIGMAGMRAMESLRLE
                     KSYRMWGSDLTPDYTPYEAGLERFVRLNKGAFIGKEALEQQLKAGVPHRFITFEVHGV
                     ADADPLGNEPLFDSKGDIIG"
     gene            2517..3551
                     /locus_tag="VGE92_03930"
     CDS             2517..3551
                     /locus_tag="VGE92_03930"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HEY1312998.1"
                     /translation="MESPAVLRKCRYAFLTLPNYSLIAVANALEPLRMANRLVGHEVY
                     EWSIVSMDGNAVHASSGLDLSPSGVLDKLGTVDILFVCGGVNVREAVSPALLTALKRL
                     AERRVPLGALCTGGYALARAGLLDHFNATIHWENLSALREEFPRVRISDQLFTIDRDR
                     FTCSGGTAPLDLMLNLIQAKLGSRISQLVSEQFVVDRVRKDTDKQYIPLRAQVGVSHR
                     GLIRVAQLMEENIEKPLSLETIAKSTGLSRRQIERLFKRDLNCVPKHYYLEMRLKRAR
                     ELLLQTAMPIMDITTACGFQSPPHFSKCYRIKFGYPPSAERKIGDGRQRPGGLANQSS
                     PEPAPCVPNR"
     gene            complement(3509..>4654)
                     /locus_tag="VGE92_03935"
     CDS             complement(3509..>4654)
                     /locus_tag="VGE92_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1312999.1"
                     /translation="TQDKLYGEDYYMPGLQYQGAAFQSYLNYYGLTQPTLPPGDWYSY
                     RERSDYLQSTEFANINFDITDRLNVEAGTVHFKSHFSYSTPYVQFAYANSLPSYYEDG
                     SKKWNSKLGINYKVTDKALVYADFAQGFRDGGSNAGDPPNCYKGGVPYTYTPDTLNNY
                     EVGWKTTWLDGRLLWNGAGYLMNWQKLQTLLYNALVCPTSSYNVNIGQARIYGMESNI
                     EYKVNSNLTLQAALSYNDSHLISSPTASYDAYVGERLPFSPYFSWSWNARYEHPLGAN
                     LHGYAQLDMAHKGDMWNNINPEDKNTGLPRILQPEYTLTNVRFGVKPAGDTWLGEFYI
                     TNLTDKNAIVYSNTGNFDLRLTTNEPRVYGVRLSYRFGTQGAGSGDD"
BASE COUNT          876 a         1427 c         1393 g          958 t
ORIGIN      
        1 ggccgatgat gtcgcctttc gaatcgaaca gcggctcgtt gccgagcgga tcggcatccg
       61 cgacaccgtg aacctcgaac gtgatgaagc gatgcggcac tcctgccttg agctgctgtt
      121 cgagcgcctc cttgccgatg aatgctccct tgttgagtcg cacgaagcgc tccaggcccg
      181 cctcataggg tgtgtagtcc ggagtcaggt cgctgcccca catgcggtat gatttttcga
      241 gccgcagcga ttccatggcg cgcatgccgg ccatgccgat gctgaactct gccccggcgg
      301 caaatagcga atcgaacaag tgatgtgcgt actcaatcgg aaaatgcagc tcccagccga
      361 gtgcaccgac gaagttgacg cgcagcaaat acacatccgt ggcgaggccg acctcggcca
      421 cttgccccgt gagccacggg aatgccgcat tgtccaacgg cgcgtccgtc agtttcgcca
      481 atacttcgcg cgagcgcggc cccgccagca cgaaaacgcc gcggctcatc gtgatattgc
      541 gcaaactgac gctgccgtcc tgcggcagca tctgctgcag gtagtcgctg tcgtagcgct
      601 cggcggcgcc ggcgctcacg acatagaagt gatcgtcggc aatcttggta atggtgaatt
      661 cgctgcggat gccgccgcgg cgggtcaacg cgtggcacag cgcgatgcgt ccgatcttgg
      721 tcggtacttt gttggccacc aagccatcga gccaactctc cgcacccggc ccgctgactt
      781 cgtgcttggt gaaaggcgtc aaatcgatga tgccgacgtg ctcgcgcaga cgccgcgcct
      841 cattgcccac atgctggaaa taattcgagc ggcgaaagct ccagtgatct ttggcaggta
      901 ctcccggcgg cgcgaaccag ttggcacgct cccagccgta gcgctggccg aacacggctc
      961 ccatgcgctg cagtttgtcg tacaccgggc tggttttggc ggggcgcgcg tcggctcgtt
     1021 cctcatccgg ataatggatg acgaatacgt tgcggtacgt ctcctcgttc ttctgcacca
     1081 cgtaacgctt gctggtgtag ggtccgtagc ggcgcggatc gaccgccatc atgtcgatgc
     1141 cgggttcgcc ctcgacgatc cactcggcca gctgccaacc cgatcctccg gcggcggtaa
     1201 tgccgaaact gtgtccttcg ttgagccaaa gattgcgtgc tccccaggcg ggtccgatca
     1261 acgggctgcc gtcgggcgtg taggcgatcg gtccgttcac gatatccttg atgccgcagg
     1321 tcgcgagtga gggtacgcgc cgttgcgccg cctcgacgtg cggcagcaat cgctccaggt
     1381 cgccctcgaa caaactcttg ccgaaccagt cgggtacgcc gtcggcgaag cgcgccggag
     1441 ccccggcttc atagggaccc aagatccaac cgaggcgctc ttcgcgcaaa taataggact
     1501 ggtccgattc gcgcagcacc gccaattctc ggccgccgcc ctgacgatag gctttgagtt
     1561 ccggcgattc gtcgtacacg atgtactggt gctcgaccgg gatcgagggc acgttgaggc
     1621 cgaacaggcg cccggtttga cgcgcgtaat ttcccgttgc acacaccacg tgctcggcga
     1681 cgatgtcgcc gcggttggtg gtcagatgcc actcaccgga ggctgtgcgt tttaccgccg
     1741 tcacttcggt ctgttcgttg atttctgcac cgcggctgcg cgcgcctttg cgcagcgcca
     1801 tggtgagatc cgccggcgcg atgtggccgt cgtcggggtg gtacagagcg ccgatgattt
     1861 tgggcgtgcc ggcgccatcg ccgagttcga ccaacggcca aaggtcgcgc acttgttgcg
     1921 ggccgatcac ctggaaaggc acgccgatgg tattggcggt gccgcagtat ttctgatatt
     1981 cgtccatccg atcgcggcag gttgcgaggc gtaaattgcc ggtgacatgg aaactcacgt
     2041 cctgaccggt ttccgccggc agccgcttgt aaagatccac cgagtatttg tgcagctggc
     2101 cgacggtgta gctcatgttg aacagcggca acaatcccgc cgcgtgccag gtcgatcccg
     2161 cggtgagttc cgcgcgctcc aacaacacca catcgctcca gcccttcaaa gccaggtgat
     2221 aaagcgtgct gacgcccacc acaccgccgc caatcacaac gacgcgggca tgacttttca
     2281 tgtgaggtct ccccaacgca aatgcgtgtc gcagtgtaac cagcgccccg ccaaacaaga
     2341 cgccaattgc gacaatccct gatggaaatg cgccaggcca ccccgaaaac gacccaaaag
     2401 cggggtgtag ggggtcttta gaccgtctgg aatgcgacaa ttgcctgtcg gaaacagaca
     2461 agataccccg cgcaatggca taattgcgcg gtccggatcc ttcccgtgga ggcaccttgg
     2521 aatcgcccgc cgtcctgcgc aagtgcaggt acgcctttct gactctgccg aactattccc
     2581 tgatcgcggt cgccaatgcg ctcgagccgc tgcgcatggc aaatcgcctg gtcggccacg
     2641 aagtttacga atggtcgatc gtcagcatgg atggcaatgc ggttcatgcc agcagcggcc
     2701 tcgatctatc gccgagcggc gtcctcgata agttaggaac ggtcgatatt ctgttcgtct
     2761 gcggcggcgt caatgtgcgc gaggcggttt cgccggcgct gctgacggcc ttgaaacgtc
     2821 tggccgagcg gcgcgtgccg ctcggcgcct tgtgcacggg cggctatgcg ctggcgcgcg
     2881 ccggtttgct cgatcatttc aacgcgacca tccattggga aaatctatcg gcattgcgcg
     2941 aagagtttcc ccgcgtgcgc atcagcgacc aattgttcac catcgatcgc gaccgcttca
     3001 cctgttccgg cggcacggcg ccgctcgatc tcatgttgaa cctcattcaa gcgaagttag
     3061 gatcgcggat ctcgcagctg gtttccgagc aattcgtcgt cgatcgcgtg cgcaaggata
     3121 ccgataagca gtacatcccg ctgcgcgcgc aggtcggtgt gtcgcatcgc ggcttgattc
     3181 gcgtcgcgca gctcatggaa gagaacatcg aaaagccgct atcgctcgaa accatcgcca
     3241 agtccaccgg cctgtcgcgc cgccagatcg agcgcctgtt caaacgcgat ctgaattgcg
     3301 tgccgaagca ttactatctc gagatgcgct tgaaacgcgc ccgtgaattg ctgctgcaga
     3361 ccgcgatgcc catcatggac atcaccaccg catgcggctt tcaatcgccg ccgcatttct
     3421 ccaaatgcta ccgcatcaag ttcggctacc cgccgagtgc cgagcgcaag atcggcgacg
     3481 gacgccaacg gcctggcggc ctcgccaatc aatcgtcgcc cgagccggca ccttgcgttc
     3541 caaatcggta agaaagccgc actccataca cgcgcggctc gttggtcgtc agccgcaggt
     3601 cgaaattgcc ggtgttgctg taaacgatcg cgttcttgtc cgtgagattg gtgatgtaga
     3661 actccccgag ccaggtgtcg cccgcgggct tcacgccgaa gcgcacattc gtgagggtgt
     3721 attcgggctg caggatgcgc ggcagcccgg tgttcttgtc ctcaggattg atgttattcc
     3781 acatgtcgcc cttgtgcgcc atgtccaatt gcgcatagcc gtgaaggttg gcgccgagcg
     3841 ggtgctcata gcgcgcattc cagctccagc tgaaataagg cgagaacggc agccgttcgc
     3901 ccacataagc gtcgtagctc gcggttgggc tcgagatcag gtgcgagtcg ttgtagctga
     3961 gtgcggcctg taaagttaaa ttgctgttga ccttgtactc gatattcgac tccataccat
     4021 agatgcgcgc ctgcccgatg ttgacgttgt aactcgacgt gggacagacc agggcgttgt
     4081 agagcaaggt ctgcagcttt tgccagttca tcaggtagcc cgcgccattc caaagcaggc
     4141 gcccgtccaa ccaggtggtt ttccagccaa cctcgtagtt gttgagcgtg tcgggtgtgt
     4201 aggtgtaggg aacaccaccc ttgtagcaat tcggcggatc cccggcgttc gagccgccgt
     4261 cgcggaagcc ctgcgcgaaa tcggcataca ccagggcctt gtcggtcacc ttgtagttga
     4321 tgcccaactt gctattccac tttttcgagc catcttcata gtaactcggc aatgagttgg
     4381 cgtaagcgaa ttggacgtag ggcgtggaat aggaaaagtg agacttgaaa tgcacggtac
     4441 ccgcttcgac gttgagccgg tccgtgatgt cgaaatttat gttggcgaac tcggtgctct
     4501 gcaagtagtc ggaacgctcc ctatacgaat accagtcgcc cggcggcagc gttggctggg
     4561 ttaggccata gtagttgagg taggactgaa acgccgcgcc ctggtactgc agtcccggca
     4621 tgtagtagtc ctcaccatag agcttgtcct gggt
//
LOCUS       DASWZT010000143         3300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_25234, whole genome shotgun sequence.
ACCESSION   DASWZT010000143 DASWZT010000000
VERSION     DASWZT010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25234"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2379
                     /locus_tag="VGE92_03940"
     CDS             <1..2379
                     /locus_tag="VGE92_03940"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEY1313000.1"
                     /translation="DYLNTLSDMLRIVGRFDESEALARRSLALAEKSGAPERIAAAAT
                     VLGILLSYSGRDAESEPYVRQALQIFEKIDGPAHPDVAVALNNLGLTLVQLGRYRESE
                     PPLQRAINIWEANLGPLHVNLTRALMNLGTALMQSGDYAGAEPDFQRAYRIAAIAGVA
                     PPVWRTPSKLMELYGNPHVAQPELAIFYGKKAVNTFQGLRGNLGGAAGAQESFLNTVE
                     PTYRKLADLLIQQGRLGEAQQVLALLKEQEYFEFIKHGAGTQPRITTLDFTPAETKLA
                     QPDAQIVALDKESAALHTKLEADGKLSAAEKKKLDGLPAQIEAAQTVFQGQVAALRKP
                     TDQKLQDLGTRYVALGKEYGALQEKFRNEGDKFSAQDHARLGELRNAMDAAQATFDAR
                     AAEVAKNSNDPEAQKRRQHEINDFSRAFQGTLKELGHDAVLAQYFILDDKVSILLTTP
                     NAVVSRESAIKRAALNAQIQSFRNSLRNPRQDPRPEAQSLYRLLVGPIADDLRQAGAK
                     TLMLSLDDTLRYIPFAALNDGKQYLIENYSLVMVTEAVRDKLAQLPIAEWTVWGLGTT
                     RAGPGYDALPWVDVELNGIAGQRGVLHGSVLLDKSFNESSLRDGLDQHYPVIHIASHF
                     QFTPGSMDDSFLLLGDGTHMTLAQIRTKLNFNDVELLTLSACDTALGDGGGAHHGVEV
                     EGLGALAQEAGAKAVLATLWPVADESTSELMRALYQEHRDRHRDKADALRAAQLDLIR
                     GTVTPDATAEDSHRSLTRSDGAPAGGNFKADPQAPFAHPFYWAPFILMGNWL"
     gene            2383..>3300
                     /locus_tag="VGE92_03945"
     CDS             2383..>3300
                     /locus_tag="VGE92_03945"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY1313001.1"
                     /translation="MSSVPETVHLKRTLGLRDTVLFIITAGTGLQWCAAAAAAGPSSL
                     ILWVIGGVLFFVPLSVCVVFLSARYPDEGGFYVWGKRAFGPFVGFMTGWTYWTANLPF
                     FPSVMYFSAGSALYWSGESDAAAHASPSYFIAFSIGALIVAAALNLRGMTIAKWLNNA
                     GAAARWVGTALLVALGIASWWRFGPATPITRRSIVPGLRLSDAIFFATIAFAWTGIEA
                     TSLMGGEIHDPKRTVPKALAIGAPMIAVIYLLGTASVLLSLPPDHTNALYGVLEAISA
                     DARRLGLPALIPIGALCASLAGLGSICLWL"
BASE COUNT          616 a         1044 c         1030 g          610 t
ORIGIN      
        1 gactacctca acacgctctc cgatatgttg cgcatcgtcg gccgcttcga tgagtccgaa
       61 gcgctggcca gaaggtcctt ggcgctcgcc gagaaaagcg gggcgcctga gagaatcgcc
      121 gccgccgcga ccgtgcttgg aattctgctc tcctactcgg ggcgcgacgc cgagtccgaa
      181 ccgtacgtgc gtcaggcatt gcaaattttt gagaaaatcg acggtccggc gcatcccgac
      241 gtcgccgtgg cgctcaacaa tttaggattg acactggtgc aactcggccg ctaccgggaa
      301 agcgagccgc cgcttcagcg cgcgatcaac atctgggaag ccaatctcgg cccgctgcac
      361 gtcaatttga cgcgggcctt gatgaatttg ggcacggcgt tgatgcaatc gggcgactat
      421 gcgggagccg agccggactt ccagcgtgcc tatcgcattg cggccatcgc cggtgtcgcg
      481 ccgcccgttt ggcgcacgcc cagcaagttg atggagctat acggcaaccc gcatgtcgcg
      541 caacccgagc tcgccatctt ctacggtaag aaagcagtta acaccttcca aggcttgcgc
      601 ggcaatctcg gcggtgcggc cggcgcacag gaatccttct tgaacacggt ggagccgact
      661 tatcgaaagc tggccgacct tttgattcaa cagggccgac tgggcgaggc gcaacaggtg
      721 ctcgccctgt tgaaggaaca ggaatacttc gagttcatca agcacggcgc gggcacccag
      781 ccacggataa cgacgctcga tttcacgccg gccgaaacca agctcgcgca accggatgca
      841 cagatcgtgg ctctcgacaa ggaatcggcc gcgttgcata cgaagctcga agccgacggc
      901 aaactgagcg cggccgagaa gaagaaattg gatgggctgc cggcacaaat cgaggccgcg
      961 cagacggtgt tccaaggcca agtggcggcg ctgcgcaagc cgaccgacca gaagctgcaa
     1021 gatctcggga cgcgctacgt cgcgctcggc aaggaatacg gcgcattgca ggaaaaattc
     1081 cgcaacgagg gcgacaagtt cagtgcccaa gaccacgcgc gcctcggcga gttgcgcaac
     1141 gccatggatg cggcgcaggc cacattcgat gcacgcgccg cggaagttgc caagaattcg
     1201 aacgaccctg aggcgcaaaa acgacgccaa cacgaaatca atgacttcag ccgcgccttt
     1261 cagggcactt tgaaagaatt gggacacgac gccgtgttgg ctcaatactt catattggac
     1321 gacaaggtgt cgattctctt gaccacgccg aacgccgtgg tgtcgcgcga gtccgcgatc
     1381 aagcgcgctg cgctcaacgc gcagatccaa agtttccgca actcgctgcg taacccgcgc
     1441 caggatccgc gtcccgaggc acagtcgctc tatcgattgt tggtgggccc gattgccgac
     1501 gatctgcgcc aagccggtgc caagactctg atgctgtcgc tcgacgatac cttacgctac
     1561 atccccttcg ccgcgctcaa cgacggcaaa caatatctta tcgagaatta ttcgctggta
     1621 atggtcaccg aagcggtgcg tgacaaactc gcgcagctgc cgatcgcaga gtggacggtg
     1681 tggggactcg gaaccacccg ggcgggtccg ggctacgatg cgctgccgtg ggtcgacgtc
     1741 gagttgaacg gtatcgccgg acaacggggc gtgctccacg gcagcgtact gctcgacaag
     1801 tcattcaacg aatcctcgct gcgcgatggc ctcgatcaac actatcccgt cattcatatc
     1861 gccagccatt tccaattcac gccggggagc atggacgatt ccttcctgct gttgggcgac
     1921 ggtacgcaca tgacgctcgc gcaaattcgc accaagctca acttcaatga tgtcgagcta
     1981 ttgacgctgt ccgcttgcga caccgcgctc ggcgacgggg gcggcgccca tcatggggtc
     2041 gaggtggagg gtctcggcgc gctcgcacag gaggccggcg ccaaagcggt cttggcgact
     2101 ttgtggccgg tggccgatga aagcacctcg gagttgatgc gcgcgctgta ccaggagcat
     2161 cgcgaccgtc accgcgacaa ggccgatgca ctgcgcgcag cacaacttga cttgattcgg
     2221 ggcaccgtga ctcccgacgc aacggccgag gacagccacc gcagtctcac gcgcagcgac
     2281 ggcgctcccg ccggcggcaa tttcaaggcc gatccgcaag cacctttcgc tcatcccttc
     2341 tattgggcac ccttcatctt gatgggcaat tggctgtagg cggtgagttc agtgcccgag
     2401 acggtgcacc tgaagcgcac gctcggcctg cgcgacacgg tgttattcat catcaccgcc
     2461 ggtaccggcc tgcaatggtg tgcggccgcg gccgccgcgg gcccgagttc gctcatcctc
     2521 tgggtgatcg gcggcgtgct gttcttcgtg ccgttgtcgg tgtgcgtcgt gtttctgtcc
     2581 gcgcgctatc ccgacgaagg gggattttat gtctggggca agcgcgcctt cgggccgttt
     2641 gtcgggttca tgacgggctg gacctactgg accgccaatc tgccgttctt cccaagcgtg
     2701 atgtatttca gcgccggcag cgccttgtac tggtcgggcg agtccgacgc ggccgcgcac
     2761 gcgtcgccga gttacttcat cgccttctcc atcggggcac tgatcgtcgc ggccgcgctc
     2821 aacctgcgcg gcatgacgat cgccaagtgg ttgaacaacg ccggcgccgc ggcgcgctgg
     2881 gtgggcaccg cgctgctggt ggccctgggg atcgcgagtt ggtggcgttt cgggccggcg
     2941 acgccgatca cccgccgcag catcgttccg ggactgcgac tgagcgacgc gatttttttt
     3001 gcgacgatcg ccttcgcgtg gaccggcatc gaggccacct cgttgatggg cggggaaatt
     3061 cacgatccga aacgcaccgt gccgaaggcg ctcgccatcg gggcgccgat gatcgccgtg
     3121 atctacttgc tcggcaccgc cagtgtcttg ctgtcgctgc ccccggacca taccaatgcg
     3181 ctgtacggtg tcttggaggc gatcagcgcc gatgcgaggc ggctcggtct gccggcgctc
     3241 atcccgatcg gcgctctgtg cgcgagtctc gccggcctcg gcagcatttg cctgtggctc
//
LOCUS       DASWZT010000144         6316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_34919, whole genome shotgun sequence.
ACCESSION   DASWZT010000144 DASWZT010000000
VERSION     DASWZT010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34919"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VGE92_03950"
     CDS             complement(<1..231)
                     /locus_tag="VGE92_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313002.1"
                     /translation="MPLFGRLLLAVALGGICALAALGASTTAPEWNTVRARPIQIGLE
                     ARRLVVGFRATDSNSEVKSFKPRAWAQNITLVQ"
     gene            451..1887
                     /locus_tag="VGE92_03955"
     CDS             451..1887
                     /locus_tag="VGE92_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007732506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY1313003.1"
                     /translation="MTYDNRQFYIDGAWVDPIEAQEFTVINPADETVAGVISMGSSKD
                     VDRAVAAARRAFDSYSRSSPAERLALMERILAAYKAHYDEIAQAISTEMGAPMTLAKG
                     SQTRIGVGHISAMIEVLKTFKFEELRGNVRLVQEPVGVCALITPWNWPMNQVAAKVVP
                     ALAAGCTMVLKPSEYSPFSAILWAKVMHEAGVPAGVFNLVNGDGTHVGAPLSAHRDID
                     MVSFTGSTRAGTEVARNAAASVKRVHQELGGKSPNVLLDDADFERAVEKSVLHVFQNS
                     GQSCNAPTRMLVPAGKMAEVEAIAKRVTDTLVKVGDPASEKTTVGPLVSKLQYERVTG
                     FIAKGIAEGAKVVTGGAGRPEGLNKGYFVKPTIFSNVRNDMTIAREEIFGPVLCILPY
                     RTEEEAVQIANDTPYGLAAYVWSQDKVRARRVGNRIRAGQVALNGASGDMQTPFGGFK
                     MSGNGREYGEFGLRDFLEVKAMIGVDAA"
     gene            1884..2585
                     /locus_tag="VGE92_03960"
     CDS             1884..2585
                     /locus_tag="VGE92_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1313004.1"
                     /translation="MTASASADAAVVRLESLSKRYSPDRPALFDGLDLELKPGEYLAV
                     MGESGVGKSTLLNLLAGLDRPDSGRVLLEGVDLSTLDDDAITLLRRRAVGFVFQAFHV
                     LPYLSVEQNVALPLDLLAIREPERSRRTLEMLAAVGIAPLARRYARELSGGEVQRIAI
                     ARALVHRPRLVLADEPTGNLDPRSAAQTLALLRAQIKANAAAGILITHSRAAAETADR
                     IVTLDAGGLKPLRSS"
     gene            2582..5113
                     /locus_tag="VGE92_03965"
     CDS             2582..5113
                     /locus_tag="VGE92_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY1313005.1"
                     /translation="MSTASETRGGFVWRILLLAQLRESPGRLAVTVLAIALGVSLGAG
                     VYLVNSAALNEFGLATKHLVGEADVVVRGPREGFSEQLFTRLAANAAVSEASPVLELE
                     AALPGRREPLKVVGVDPFRAATLQPALLGDIGGGVLTLLQTDSIYLSSSAAQQLHLQR
                     GDMLQVIVASAPKALHVVGILPETNYSQPLGLMDIAQAQWAFNEIGVLNRIDLRLGAG
                     TDVDAFRRSLRATLPAGVLAIAPQVERDRAISVTRAYRVNLNMLALVALWTGAFLVFS
                     TQSLSVLRRRRSLALLRALGVTQRQLQRALIGEGAALGVAGSLLGVILGCGLAAVILE
                     FLTGDLGNGQLRAVGASLRAASLPMLGFFVMGIVVASVGAWLPARNASRQAPARSLKG
                     GDADYGGVARASARSGVALLALGAVLAPLPAVRGLPLFGYAAIAALLFGAVLLVPALT
                     VLALSLAPRTRRIVLDTAVAQLRENVGLSTLSLASIIVSFSLMVAMAIMVYSFRVSFE
                     HWLDRLLPADMQLREPFGSDTAYWSSADQAALASVAGVARIEFRRTRQLLLDPAHAPV
                     TLIARGASDAQAAGELPLLRAAKLPPASNAAPAWISEALEDVYGYRIGDRIDLPLAGR
                     SQPFSVAGIWRDYARPAGAIVISRAAYRAATSDPSANEGSVWLHSGANPAAVEALLRA
                     RFARGDSLEILTSTALRERSLQIFDRAFAITYALEAIAVIIGLTGISFAASSTALARR
                     AEFGMLRHIGMLRRQIVGMLASEGILTSAFGVIYGLVLGAALSLVLVYVVNRQSFNWS
                     IDLAVPAWQLAVLSVTLVAAAAITAIWSGRAAMSQDAVQAVREDW"
     gene            5110..6234
                     /locus_tag="VGE92_03970"
     CDS             5110..6234
                     /locus_tag="VGE92_03970"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEY1313006.1"
                     /translation="MRQRLSAAGWVAALLAAALVSFGAAAAAPDAPAYAPAYAPAYAP
                     VVAGHPLVFPADFGSHPQFRTEWWYVTGWLSTDQGEPLGFQITFFRTKPAIDDNNPSA
                     FTPHQLMVAHCAISDPKRGRLWQDQRIRRTGMGLAEAAIGDTHVWIDRWSLKREPRAY
                     TAKISAEEFSFDLTLIETQAALLNGDAGVSRKGPAAEAASYYYSVPHLKVVGSIVRQG
                     RTDAVKGEAWLDHEWSSEYLDEEALGWDWIGLNLDDGASLMAFRIRGLRGEARWAGGT
                     LRSSDGKVQILEPADIDFRPGRRWLSAQTGITYPVQWSVRAGARQIDLEPLLDNQEND
                     TRLSTGAIYWEGAVRAYEHQKLVGRGYLELTGYGEKLKLP"
BASE COUNT         1028 a         2027 c         2117 g         1144 t
ORIGIN      
        1 ctgcacgaga gtgatgttct gcgcccaggc acgcggtttg aacgatttga cttccgagtt
       61 gctgtcggtg gcgcgaaagc caaccaccaa acgccgcgcc tcgagtccga tctggatggg
      121 gcgcgcacgc accgtattcc actccggcgc cgtggtgctc gcgcccagtg ccgccagggc
      181 acagataccg cccaaggcaa cggcgagcaa cagtcttccg aataagggca taaacactcc
      241 aaataccgac tcgagcgagg ccaaatcgca cgcagcctaa caataatact caggccaaaa
      301 tgcgaactac gaccccaaaa cccccgcagc cccgcctcgc ctcccggccc gggaagcgag
      361 cgtcggtggt tggcgacaac accctgcgcg gcgcagggcc agcgggtaga atggcgcggc
      421 ggtaatcaat gcaaggtgag gggcaattga atgacgtacg acaatcggca gttctacatt
      481 gacggtgctt gggtcgaccc gatcgaggcg caggagttca cggtcatcaa cccggccgac
      541 gagaccgtgg cgggtgtcat ttcgatgggc agctccaaag atgtggatcg cgccgtggcg
      601 gcggcccgcc gcgccttcga cagctactcg cgcagctccc cggcagagcg cctggcgctc
      661 atggagcgca tcctcgccgc ctataaggcg cactacgacg agatcgcaca ggcgatctcc
      721 accgagatgg gtgcgccgat gaccctcgcc aagggctcgc agacgcggat cggtgtcggc
      781 cacatcagtg ccatgatcga ggtgctcaag accttcaaat tcgaggagct gcgcggcaac
      841 gtgcggctgg tgcaagaacc ggtcggtgtc tgcgccctga tcacgccgtg gaattggccg
      901 atgaaccaag tggcggcaaa agtggttccg gcgctcgccg ccggctgcac catggtgctc
      961 aaaccctcgg agtattcccc gttcagcgcc atcctctggg cgaaggtgat gcacgaggcg
     1021 ggcgttcccg ccggcgtatt caatttggtg aatggcgacg gcacccacgt cggcgcgccg
     1081 ttgtccgcgc atcgcgacat cgacatggtc tccttcactg gctcgacgcg ggccggcacg
     1141 gaagtcgcgc gcaatgccgc ggcgtcggtc aagcgcgtgc atcaggaact cggcggcaaa
     1201 tcgccgaacg tattgttgga cgatgccgac ttcgagcgcg ccgtggaaaa gagcgtgctg
     1261 cacgtgttcc aaaattccgg acagtcgtgc aatgcgccga ctcgcatgct ggtgcccgcg
     1321 ggcaagatgg ccgaggtgga ggcgattgcc aaacgcgtca ccgacacgct ggtgaaggtc
     1381 ggcgatcccg cctcggagaa aacgaccgtc ggcccgctgg tctccaaatt gcaatacgag
     1441 cgcgtgacag gcttcatcgc caagggaatt gccgaaggag cgaaggtcgt caccggcggc
     1501 gccggccgtc ccgagggcct gaacaagggc tatttcgtca aaccgacgat tttctccaat
     1561 gtgcgcaacg acatgacgat tgcccgcgag gagatattcg gcccggtgct gtgcatcctg
     1621 ccctaccgga ccgaagagga agcggtgcag atcgccaacg acacgcccta cggcttggcc
     1681 gcctatgtct ggtcgcagga caaggtgcgt gcgcgacgcg tcgggaatcg cattcgcgcg
     1741 ggccaggtgg ccttgaatgg cgcctcgggc gatatgcaaa ctcccttcgg cggtttcaag
     1801 atgtccggca acggccgcga atacggcgag ttcggcttgc gtgatttcct cgaggtcaaa
     1861 gccatgatcg gagtcgatgc cgcttgacag cgtcggcctc ggccgatgct gccgtcgtgc
     1921 ggctcgagag tctgagcaaa cgctattcgc cggatcgccc ggcgctgttc gacggtctcg
     1981 acctcgaact caagccgggc gagtatctcg cggtgatggg agagtccggc gtcggcaagt
     2041 cgacgctgct caacttgctg gccgggctcg atcggccgga ctcgggtcgc gtgctgctcg
     2101 agggtgtcga tctatcgacg ttggatgatg atgccatcac gttgctgcgc cgccgggcgg
     2161 tgggcttcgt gtttcaggcc tttcatgtgc tgccctattt gagcgtcgag caaaatgtcg
     2221 ccttgcctct cgatctgttg gcgattcgcg agccggagcg ctcacgccgc acgctcgaga
     2281 tgctggccgc ggtcggcatc gcgccgctcg cccgccgcta cgcacgcgag ttgtcgggcg
     2341 gcgaagtaca gcgcatcgcg atcgcgcgcg cgctggtgca tcggccgcgc ttggtgttgg
     2401 cggatgagcc gacgggaaac ctcgacccgc gcagcgccgc gcaaaccctg gcgctgctgc
     2461 gcgcacagat caaggcgaac gccgctgccg gcatcctgat cacccactcg cgtgcggccg
     2521 ccgagacggc cgatcgcatc gtcacgctcg atgcgggtgg cttgaagccg ctgcgctcgt
     2581 catgagcacg gcgagcgaga ctcggggcgg gttcgtgtgg cggatattgc tgctcgcgca
     2641 gctgcgcgag tcgccggggc gcctggcggt cacggtgctc gcgattgcgc tcggcgtgtc
     2701 cttgggcgcc ggggtgtacc tcgtgaacag cgccgcgctc aacgagtttg gcttggcgac
     2761 caagcatttg gtgggagaag cggacgtggt ggtgcgcgga ccgcgggaag gcttctcgga
     2821 gcagcttttc acgcgcttgg ccgccaatgc cgcggtcagc gaggcgagcc ccgttctgga
     2881 actcgaggca gcgctgccgg ggcggcgcga gccgctcaag gtggtgggcg tggacccgtt
     2941 ccgcgccgcg acgctgcaac ccgcactgct cggcgacatc ggcggcggtg tactcacgtt
     3001 attacaaacg gacagcattt atctgagcag cagcgccgcg cagcagctgc atttgcagcg
     3061 cggcgacatg ctgcaggtca tcgtggcaag cgcccccaag gcgctgcacg tggtgggcat
     3121 tctgccggaa accaattatt cgcaaccctt gggactgatg gatatcgccc aggcgcaatg
     3181 ggccttcaac gaaatcggcg tactcaatcg catcgatctg cgcctcggcg cgggcaccga
     3241 tgtcgatgcc tttcgccgct cgcttcgcgc caccctcccc gccggcgttc tcgcgatcgc
     3301 gccgcaggtc gagcgcgacc gtgcgatcag cgtgacccgt gcctatcggg tgaatctcaa
     3361 catgctggcg ttggtggcgc tttggaccgg cgcgttcttg gttttctcga cacagtcgct
     3421 gtcggtgctg cgacgccgcc gctcgctggc gttgctgcgg gctctcgggg tgacgcagcg
     3481 tcaattgcag cgcgcgctca tcggcgaggg cgccgcactc ggcgtcgcgg gttcgttgct
     3541 cggcgtgatc ctgggttgcg gcttggcggc cgtgattctc gaatttctga ctggcgatct
     3601 cggcaacggt caattgcgcg cggtcggcgc ctcgctgcgc gcggcgagct tacccatgct
     3661 ggggtttttc gtgatgggga tcgtggtggc gagtgtcggt gcctggctgc cggcgcgcaa
     3721 tgcctcacgc caagcaccgg cccgctcctt gaagggcgga gatgcggact acggcggcgt
     3781 cgcacgcgcg agcgcccgct cgggcgtggc cctgctggcg ttgggcgccg tgctcgctcc
     3841 cctgcccgcc gttcgcggcc tgccgttgtt cggctatgcc gcgatcgccg cgctgctctt
     3901 cggtgcggtg ctgttggtcc cggccctgac cgtgctcgcc ttgagtctcg cgccgcgcac
     3961 gcgccgcatc gtgctcgaca cggcggtggc gcagctgcgc gaaaacgtcg ggctgtcgac
     4021 tttgagcttg gcctcgatca tcgtgagttt cagcctgatg gtggccatgg ccatcatggt
     4081 gtactcgttt cgtgtttcat tcgagcattg gctcgaccga ctcctgcccg ccgacatgca
     4141 attgcgcgag cccttcggca gcgacacggc ctattggtcg agcgccgatc aggcggcgct
     4201 cgcatcggtt gcgggcgttg cgcgcatcga gtttcggcgc acgcgccagc tgctgctcga
     4261 tcccgcgcac gcgccggtga ccttgatcgc gcgcggcgcg agcgatgccc aagcggccgg
     4321 cgagctgccg ttgctgcgcg ccgccaagct gccaccggcc tcgaacgcag cgccggcctg
     4381 gatttccgag gcgctcgagg atgtctacgg ctaccggatc ggcgatcgga tcgacctgcc
     4441 gctcgccgga cgcagccaac cgttttccgt ggcaggcatc tggcgcgact atgcgcgccc
     4501 tgccggcgcc atcgtcattt cgcgcgcggc gtatcgagcg gccacttcgg acccaagcgc
     4561 caatgaggga tccgtgtggc tgcactcagg ggcgaacccc gcggcggtcg aagctttgct
     4621 gcgcgctcga ttcgcgcgcg gcgattcatt ggagatcttg acgagcacgg cgctgcgcga
     4681 gcgctccctg cagatattcg atcgggcctt tgccatcacc tacgccctgg aggcgatcgc
     4741 ggtcatcatc ggtctcacgg gcatcagctt cgccgcgagt tcaaccgccc tcgcccgtcg
     4801 cgccgagttc ggcatgctgc ggcacatcgg catgctgcgc cggcagatcg tcggcatgtt
     4861 ggcgagcgag gggattctga cgagcgcatt tggagtcatt tatggcttgg tactcggcgc
     4921 ggcgttaagt ttggtgttgg tgtacgtggt caatcgccaa tccttcaatt ggagcatcga
     4981 tctggccgtt cccgcgtggc agcttgccgt gctgagcgtg acgctggtgg cggcggccgc
     5041 gattaccgca atctggagcg gacgcgccgc gatgagccag gacgccgtgc aggccgtgcg
     5101 cgaggattgg tgagacagcg cctctcggcc gccggctggg tcgctgcgct cctcgctgcg
     5161 gcgctggtga gcttcggcgc ggcggcagcg gcgcctgacg cgcccgcgta tgcgcccgcg
     5221 tatgcgcccg cttacgcgcc ggtcgtcgcc ggccacccgc tggtgtttcc ggcggatttc
     5281 ggcagccatc cgcagtttcg caccgaatgg tggtacgtca ccggctggtt gagcaccgat
     5341 cagggcgaac cgctcggctt tcaaatcacg ttctttcgca ccaagcctgc gatcgatgac
     5401 aacaatccga gcgccttcac cccgcatcag ctgatggtcg cgcactgcgc catcagcgat
     5461 cctaagcgcg gccgcctgtg gcaggaccag cggattcgcc gcacgggaat gggtctggcc
     5521 gaggccgcga tcggcgatac ccacgtgtgg atcgatcgct ggtcgctcaa gcgcgagccg
     5581 agggcttaca ccgcgaagat cagcgccgag gagttttcat tcgacctcac gctcatcgag
     5641 actcaagcag cgctgctcaa cggcgatgcc ggcgtcagcc gcaagggccc cgccgccgag
     5701 gccgcaagct attactacag tgtgccgcat ctcaaagttg tgggcagcat cgtgcggcaa
     5761 ggcaggactg acgccgtcaa gggcgaagcc tggctcgatc atgaatggtc cagcgagtac
     5821 ctagacgagg aggctctcgg ctgggattgg atcggcctca acctcgacga cggcgcctcg
     5881 ctcatggcgt ttcggattcg cggccttcga ggcgaagcgc gttgggccgg cggcactctg
     5941 cgcagcagcg acggcaaggt gcagattctc gagccggccg acatcgattt tcgccccggg
     6001 cgacgctggc tgtctgcgca aacgggaatc acctacccgg tgcagtggtc ggtgcgcgcc
     6061 ggcgcccggc agatcgatct cgaaccgctg ctcgacaatc aagagaacga cacccggctc
     6121 tcgaccggag cgatctattg ggagggtgcg gtgcgggcgt atgagcatca aaagctcgtt
     6181 ggccgcggct atcttgaact aaccggttat ggcgagaagc tgaagttgcc ataatcgcac
     6241 gtgacatcaa agtgtcacct ccctgcatca gagtggaaag gggcccgcat ttgggaaacc
     6301 cgcaccatga agacac
//
LOCUS       DASWZT010000145         4246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_59137, whole genome shotgun sequence.
ACCESSION   DASWZT010000145 DASWZT010000000
VERSION     DASWZT010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59137"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..1401
                     /locus_tag="VGE92_03975"
     CDS             76..1401
                     /locus_tag="VGE92_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY1313007.1"
                     /translation="MKNRPSGFGIEKLPRISHGTGSYLFDEAGKRYLDGSGGPAVFCL
                     GHAHPEVNAAISRQLNDIAYGYRYLFTSAALEDLTSALLRLSGGGFQDIVYSGSGSEA
                     VESALKIALQHFAARGMMSKRRFIARRRSWHGDSLGALSISDFLQRRRAFEGSLLEVT
                     FVSAANAYRPPDGVSGETLVKHLAAELERAILSLGAEHVAAFVFEPIVGAAGGVVPAP
                     PGYAQAMQAVCNRYDVLLIADEVMCGSGRSGTWCALQHDNVVPDIMSIAKGLAGGYIP
                     LGATLVTARVAAPILSEHGAFMTGHTFSGHTAACAAALAVQLIIERDRLLERVHDTGA
                     RLQQALREALSKFDEVGDVRGRGFFIGIELVRDRRTKQPFAAERALSFEIGARAFADG
                     LICYPCAGNVAGTAGDTIILAPPYNSSDGELEELVTKTVRAVGAALSGA"
     gene            complement(1387..1842)
                     /locus_tag="VGE92_03980"
     CDS             complement(1387..1842)
                     /locus_tag="VGE92_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY1313008.1"
                     /translation="MFKRVLLCYDGSAVGRRALKRGADLAILVGAQVYVLSIVPSNVA
                     DAAVVAGAAGHACLIDEADGYRKVLDESIEWLKARGVHTEGYLASGNTIEQIIAFSRR
                     LAIDLIVLGHYPQPTGGFWWSNPHRSSLAERANCCVFVAVADDAPSSAA"
     gene            complement(1847..2608)
                     /locus_tag="VGE92_03985"
     CDS             complement(1847..2608)
                     /locus_tag="VGE92_03985"
                     /inference="COORDINATES: protein motif:HMM:NF017551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HEY1313009.1"
                     /translation="MCLILVVWRADSRYPCVVAANRDELHGRPSERAGWWADRPQILA
                     GRDLEAGGTWLGLTRRGRFAAITNYRDARLPRKNGPSRGALVTAVLESDAPVAECLTY
                     LRGVAARYNGFNLIFSDGERLGIYESVPQRGRELGPGVYGLSNHLLDSPWPKVQNAKS
                     RLKAALAHMSDTAPLLDVLRDDRPAPDAELPRTGLSLEWERLVSSAFVRAPDYGTRCS
                     TIIRIEHRGRAYFDEWSWSSDGEEIGRSCLQFRLE"
     gene            complement(2621..3847)
                     /gene="glcF"
                     /locus_tag="VGE92_03990"
     CDS             complement(2621..3847)
                     /gene="glcF"
                     /locus_tag="VGE92_03990"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: protein motif:HMM:NF008434.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEY1313010.1"
                     /translation="MKTHLSAEFDGTAAGEEAQAIIGKCVHCGFCNATCPTYQLLGDE
                     LDGPRGRIYLIKQMLEGGEATRSTQLHLDRCLTCLNCQSTCPSGVEYGRLVDIGRKIV
                     DARVERPAAERGARWLLGEGLTSPLFAPALRVGQALRPLLPAALKDKIPPRPERRAAG
                     NSGAHPRKVLLLSGCVQPAMMPNIDAATVRVLDAAGIETLRASGTGCCGALRTHLGNQ
                     EGGRADMRRNIDAWMPLISGGGVEAIISSASACSLALKDYGHALSSDTGYADKAMRIS
                     ALARDLSELLPDITAVLKERKRSAVQRLVFHPPCTLQHGQALRGGVEMHLRSLGFEVE
                     VANSESYLCCGSAGTYSILQPDLATRLRDRKLEQLTRAGPQVIVSANVGCIQHLQSGT
                     SIPVRHWIEVLDESLH"
     gene            complement(3854..>4246)
                     /locus_tag="VGE92_03995"
     CDS             complement(3854..>4246)
                     /locus_tag="VGE92_03995"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY1313011.1"
                     /translation="ELEPAAALNWWQSLRDQTEPFFSLTDADLARGERLWRLSVPSSA
                     APLQLPGRQLIEWHGAERWWRSVAPAADVRAAAAKAKGHATLVRGADKSCGVFTPLSD
                     ALLRIHRGLKQAFDPEGILNPGRMYVEF"
BASE COUNT          786 a         1380 c         1334 g          746 t
ORIGIN      
        1 cagatgaggc aggcggcgcc atgcgatata ttcgcacatc gtccggaggg ccggcgtttc
       61 tcgcggagaa aatgcatgaa aaatcgaccg agcgggtttg gaatcgagaa gctgccgcgg
      121 atctcgcacg gcacggggag ctacttgttc gacgaggcgg gaaagcgcta tctcgacggc
      181 tcgggaggtc cggcggtgtt ctgcttagga catgcccacc ccgaggtcaa cgcggccatc
      241 agccggcagc tgaacgatat tgcgtacggt taccggtatt tgttcacgag tgcggcgctg
      301 gaagatctca cctcggcgct gctgcgtctc tcgggcggcg gctttcaaga catcgtctac
      361 agcggcagcg gctccgaagc cgtcgaatca gccctgaaaa tcgcgcttca gcattttgcg
      421 gcgcgcggaa tgatgagcaa gcgccgcttc atcgcgcggc ggcggtcatg gcacggcgac
      481 agcttgggtg ccttatccat ctcggatttt ttacagcggc gccgcgcctt cgaaggcagt
      541 ttgctcgagg tgacgttcgt ctccgccgcg aatgcctacc ggccgccgga cggcgtatcc
      601 ggcgaaactc tggtcaaaca tctggccgct gaactcgagc gcgccattct ctcgttggga
      661 gcggagcatg ttgccgcttt tgtcttcgaa cccatcgtcg gtgccgccgg cggcgtggtt
      721 cccgcgccgc cgggctatgc gcaggccatg caggcggtat gcaaccggta cgacgtcttg
      781 ctgatcgcgg atgaggtgat gtgcggttcc ggacgcagcg gcacgtggtg cgcgttgcag
      841 cacgataatg tcgttccgga catcatgagc atcgccaagg ggctcgccgg cggctacatc
      901 ccgttgggtg cgacgctggt tacggcgcga gtcgccgccc cgatcctgag cgagcacgga
      961 gcattcatga ccggacatac cttcagcggc cacaccgcag cctgcgctgc ggcgctcgcg
     1021 gtgcagctca tcatcgaacg tgatcggctg cttgaacggg ttcacgacac cggcgcccgc
     1081 ttgcaacaag cgctgcgcga ggcgttgtca aaattcgacg aggtcggcga tgtccggggc
     1141 agaggattct tcatcgggat cgaattggtc cgcgaccggc gaaccaagca gccgtttgcc
     1201 gcggaacgtg ctctcagttt cgagatcggc gcgcgcgcct ttgccgacgg cttgatctgt
     1261 tatccctgtg ccggcaacgt cgcgggaacg gccggcgaca ccatcatcct ggccccgccc
     1321 tacaattcca gcgatggcga attggaggag ctggtcacca agaccgtgcg agccgtcggt
     1381 gccgctctaa gcggcgcttg acggcgcgtc atcggcgacc gcgacgaaga cgcagcagtt
     1441 ggcgcgctcc gccaacgaag agcgatgcgg gttgctccac caaaatcctc ccgtcggctg
     1501 cgggtaatgt ccgagcacga tcaggtcaat ggcgagccgc cgcgaaaagg cgatgatttg
     1561 ctcgatggta ttgccgctcg ccaaatagcc ttccgtgtgg acgccgcgag ccttcagcca
     1621 ttcaatggat tcatcgagaa cttttcgata cccatcggcc tcgtcgatga gacaggcgtg
     1681 gccggcagcg ccggcaacca ccgcagcatc cgccacattg ctgggaacga tcgacaacac
     1741 gtagacctgt gcaccgacga gtattgccag atctgcgccg cgtttcagcg cccgccgacc
     1801 taccgcggaa ccgtcatagc agagcagcac ccgcttgaac ataacgtcac tccagtcgga
     1861 actgcaggca actcctgcca atctcctcac cgtcggagct ccagctccac tcgtcaaagt
     1921 atgcgcggcc tcggtgctca atgcgaataa ttgtcgagca gcgcgtgccg tagtccggcg
     1981 cgcgtacaaa ggcgctcgag accaagcgtt cccactcgag actcaagccg gtgcgcggca
     2041 gctcagcatc gggtgccggc cggtcatcgc gcaatacgtc gagcaggggt gcggtgtccg
     2101 acatgtgagc gagtgccgcc ttcaaccggc tcttggcgtt ttgaaccttg ggccacggcg
     2161 aatcaagcag atgattcgac agaccgtaga cgccgggtcc gagttcgcgt ccgcgttgcg
     2221 ggacgctttc atagatcccc agccgctcgc cgtcggagaa gatgagattg aacccgttgt
     2281 agcgcgccgc cacccccctt aaataagtca aacactccgc gaccggggcg tcggactcga
     2341 gcaccgcagt caccaacgcg ccgcggctcg ggccattctt gcgcggcagg cgcgcgtcgc
     2401 gataattcgt gattgcagcg aagcggccgc ggcgcgtcag gccgagccac gtgcctccgg
     2461 cctcgagatc ccgccccgca agtatctgcg ggcgatccgc ccaccacccg gcgcgctcgc
     2521 tcgggcgccc atgaagttca tcgcggttcg ccgcaacgac acacgggtag cgcgagtccg
     2581 cgcgccaaac cacgaggatc agacacatcg acgccggctc ctagtgcaaa ctctcgtcca
     2641 atacttcgat ccaatgcctg accggtatcg aagtaccgga ctgtaaatgc tggatgcagc
     2701 ctacgttggc cgaaacgatg acttgcggcc cggcccgcgt cagttgttcg agtttgcggt
     2761 cgcgcaatcg agtcgcaaga tccggctgca gaatcgaata agtacccgcg gagccgcagc
     2821 acaggtagct ttcactattc gcgacttcga cctcgaatcc caagctgcgc aggtgcattt
     2881 cgactccgcc gcgcagcgcc tgaccatgct gcaacgtgca gggcggatga aacacgagcc
     2941 gttgcacggc gctgcgcttc cgctccttca gaactgccgt gatgtcgggc agcagttcgc
     3001 tcaaatcccg tgcgagcgca ctgatgcgca tcgccttgtc ggcatagccc gtatcgcttg
     3061 acagggcgtg accgtagtcc ttgagcgcga ggctgcaagc cgacgcagaa gagatgatgg
     3121 cttcaacgcc gccgccggaa atgagcggca tccaggcgtc gatgttgcga cgcatgtccg
     3181 cgcgaccgcc ctcttgatta cccagatgag tgcgcagcgc gccgcaacag ccggtgccgc
     3241 tcgcgcgcag cgtctcgatt ccggccgcat ccaagacgcg cacggtggcg gcgtcgatgt
     3301 tcggcatcat ggcaggctga acgcagcccg acagcagcaa caccttgcgc gggtgcgcgc
     3361 cgctgttgcc agccgcacgg cgctccgggc gaggcggaat cttgtccttg agcgccgccg
     3421 gcaacagcgg ccgcagcgcc tggcccacgc gcagtgccgg cgcaaatagc ggcgaggtga
     3481 gaccttcccc aagcaaccaa cgagcgccgc gctcggccgc cgggcgctcg acgcgcgcat
     3541 cgacaatctt tcgaccgatg tcgaccagac ggccatattc gacgccgctc ggacaggtgc
     3601 tctggcagtt aagacaggtc aggcagcgat ccaagtgcag ttgcgtactg cgtgtcgcct
     3661 cgccgccctc cagcatctgc ttgatcaggt aaatccgccc gcgagggccg tcgagctcat
     3721 cgccgagcag ttgataggtg ggacaggttg cgttgcagaa cccgcaatgc acgcattttc
     3781 cgatgatcgc ctgtgcctct tcgcccgcgg cggtgccgtc aaattcggcg ctcagatgcg
     3841 ttttcattgg ggactagaac tcgacgtaca tgcggccggg gttaagaata ccctcaggat
     3901 cgaatgcctg tttcaaaccg cggtgaatgc gcagcagcgc gtccgacagg ggggtaaaca
     3961 cgccgcagga cttgtcggcg ccgcgcacca gcgtggcatg acctttcgcc ttggccgcag
     4021 cagcgcgcac gtcggcggcg ggggccacgc tgcgccacca ccgttcggcg ccgtgccatt
     4081 cgatcaattg ccgacccggc agctggagcg gcgcggcact gctcggcacg gatagccgcc
     4141 acaaacgctc gccgcgtgcg agatcggcgt cggtgagcga aaagaacggc tcggtttgat
     4201 cgcgcagact ctgccaccaa ttcagcgctg cagccggctc gagctc
//
LOCUS       DASWZT010000146         2903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_25262, whole genome shotgun sequence.
ACCESSION   DASWZT010000146 DASWZT010000000
VERSION     DASWZT010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25262"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1463)
                     /locus_tag="VGE92_04000"
     CDS             complement(<1..1463)
                     /locus_tag="VGE92_04000"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEY1313012.1"
                     /translation="MAAISRRPRLPQYAALALSLLMAACSLVRQEQAPEDKQERAQRL
                     AHDGKHADAADAYSELATLQPASHDNYELLSAEQWLGAGNVEAAKKSFAAVSPDARTR
                     LPTERALVAAEIAYAENDGAGAIHELDQIPVPTAADQAQNYFWIRGRSAFLTGHPVEG
                     TRALVEREHFLSDAASLRANRAELYSRITSAAEHGQPMKVPPKSDPIVAGWLELGPVA
                     VGLARDPTRAATALANWRRLYPQHPANDSVLSGASTQIAAATEFPDQIALLLPLSGRA
                     ESVGVAVRDGFVAAYLEQAAASRPRLRIYDVAAESVSSAYAQAIADGAGFVVGPLTKE
                     DVAAVVPLSGARTPVLALNFVADSMATPRNFYQFALSPEDEARSVARRVVADGRMKGV
                     AIVPSGEWGSRVVAAFADELSHLGGTVLDSQRYETSQVDFSDMIKQVLQVHVVKGEPA
                     THRTDAAFVFVAAASAGVARQILPQLKFHYAGDVPVYS"
     gene            1505..2383
                     /gene="rsmI"
                     /locus_tag="VGE92_04005"
     CDS             1505..2383
                     /gene="rsmI"
                     /locus_tag="VGE92_04005"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007989873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEY1313013.1"
                     /translation="MTNAAAGRLYVVATPIGNLGDLSARARATLQSCCVIAAEDTRHT
                     GMLLKHFGIQTPQLPLHDHNEKQRAADIIERLRQGASVALVSDAGTPAISDPGFELVR
                     SVAAAGFEIVAVPGPCAAIAALSIGALPTDRFCFEGFLPARGAARRKRLTLLAGESRT
                     LVLYEAPHRVRETLEDCAQVFGGGRSATVAREITKLHEATYRGSLSELKARAQTDADF
                     GRGEIVLLIAGAPLMPHSGAEADGHGGALDRVLKILLAELPLKQAARLAAQITEARDN
                     QAYKRALRLKEESASH"
     gene            2412..2785
                     /gene="rnpB"
                     /locus_tag="VGE92_04010"
     ncRNA           2412..2785
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VGE92_04010"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          517 a          919 c          978 g          489 t
ORIGIN      
        1 gagtacaccg gcacgtcgcc ggcgtagtga aatttcagct gcggcaggat ttgccgggcg
       61 actccggcgg aggcggcggc cacgaacacg aacgcggcat ccgtgcgatg cgtggccggc
      121 tcgcccttga cgacatgcac ctgcagaacc tgtttgatca tatcggagaa gtccacttgc
      181 gaggtctcgt aacgttggct gtcgagcacg gtgccaccga gatgtgacaa ttcatcggca
      241 aaagccgcga cgacgcgact gccccattcg ccgctcggca cgatcgccac ccctttcatc
      301 cggccatccg ctacgacgcg gcgcgcgacg ctgcgtgcct catcctccgg cgacaatgcg
      361 aattggtaaa aattgcgcgg cgtcgccatc gagtcggcta cgaagttcaa agcgagcacc
      421 ggcgtgcgcg cgccgctcaa cggcacgacg gccgcaacgt cttccttggt gagggggccg
      481 acgacaaaac cggcgccgtc cgcgatggct tgggcgtagg cgctgctcac cgattcggcg
      541 gcaacgtcgt agattcgcag gcgcgggcgg ctcgcggccg cttgttccag gtaagccgcg
      601 acgaagccgt cgcgcacggc cacgccgacg ctttcggcgc gccccgacaa cggcagcaac
      661 agcgcgatct gatccggaaa ttccgtagcg gcggcgattt gcgtcgatgc gccggaaaga
      721 acgctgtcgt tcgccggatg ttgcgggtag aggcggcgcc aattcgccaa agccgtggcc
      781 gctcgcgtgg ggtcgcgcgc gagtcccacg gcgacgggtc ccaactccag ccagcccgcg
      841 acgatgggat cggacttggg cggcactttc atcggctgac cgtgctccgc ggcgctcgtt
      901 atcctcgagt acaactccgc gcggttggca cgcaggctcg cagcatcgct cagaaaatgt
      961 tcgcgctcga ccagcgcgcg cgtgccttcg accggatgtc ctgtaagaaa ggcgctgcga
     1021 ccgcggatcc agaaatagtt ctgcgcctga tcggcggccg tcggcacggg aatttgatcg
     1081 agctcgtgga tcgcgcctgc gccgtcgttc tcggcatagg cgatttccgc ggccaccaag
     1141 gcgcgctcgg tcggcaagcg ggtgcgtgcg tcgggggaaa ccgccgcgaa cgatttcttc
     1201 gcggcctcga cattgccggc gccgagccat tgttcagcgc tcagcagctc gtaattgtcg
     1261 tggctggccg gttgcagggt ggcaagttca gaatacgcat ccgccgcgtc cgcgtgcttg
     1321 ccgtcgtggg cgaggcgctg cgcacgctcc tgcttgtcct ccggcgcctg ttcctgtcgc
     1381 accaggctgc aggcagccat tagcaaggat agtgcgagcg ccgcatactg cggcagccgt
     1441 ggacgtcgag agatcgctgc catacccttc cactcctgat ccaaaacttg aggcacggat
     1501 tatagtgact aatgctgcgg cggggcggtt gtacgtagtc gcgactccga tcggcaattt
     1561 gggcgatctg tcggcgcgcg cgcgcgcgac tttacaaagt tgctgcgtca tcgccgccga
     1621 agacacgcgc cataccggca tgctgttgaa gcatttcggc attcagacgc cgcagttgcc
     1681 gctgcacgat cacaatgaaa agcagcgcgc cgcagacatc atagagcggc tgcggcaagg
     1741 cgcgtcggtc gcgctggtga gcgatgccgg tacgcccgcc atcagcgacc cgggcttcga
     1801 attggtccgc agcgtggcag cggctggatt cgagatcgtc gccgttcccg ggccgtgcgc
     1861 cgcaatcgcc gcgctgtcga tcggcgcgct gccgactgat cgcttctgct tcgagggttt
     1921 cttgccggcg cgcggcgcgg cgcggcgcaa gcgtttgacc ctgctggcgg gtgaatcgcg
     1981 cactctcgtg ctgtacgaag cgccgcatcg ggtgcgcgag acgctcgagg attgcgccca
     2041 agttttcggc ggcgggcgca gcgccacagt ggcgcgcgag atcaccaagc tgcatgaagc
     2101 gacgtaccgc ggttcgctct ccgaactcaa ggcacgggcg caaacggacg ccgatttcgg
     2161 ccggggtgaa atcgtattgc tgatcgccgg tgcgccgcta atgccgcaca gcggcgccga
     2221 ggccgatggc catggcggcg ccctcgaccg ggtgctgaaa atcctgctgg cggagttgcc
     2281 gctgaaacag gcggcgcgcc tggcggcgca gatcaccgag gcacgcgaca accaagccta
     2341 taagcgcgcg ctgcgtctaa aagaagaatc tgcctcgcat taggcgccat atctgcgtat
     2401 ggtgctgccc aagagtcggc caggcagtcg ctgtgtgcgc aagcgcatgg aggaaagtcc
     2461 gggctccata gggcacggtg ccaggtaacg cctggggggc gcgagcccac ggaaagtgca
     2521 acagaaagat accgcctaag taccgcgcga gcggtgccgg caagggtgaa atggtgcggt
     2581 aagagcgcac cgcgcaggtg gcaacacagg cggcacggca aaccccacct ggagcaagac
     2641 caaataggga agcacgcggt gcatgtcggc cgcaccatgt ggtccgcatg gggctttcgg
     2701 gtaggtcgct agagctgcgc ggcgacgcgc atcccagagg aatgactgtc cacgacagaa
     2761 cccggcttaa cggccgactc tttttttagc gctgacgcac ccctgctggt gccgatgcgc
     2821 gctcagggca ggcagggcat gcgtgtggta aaactcgccg aaggctagct ggcggtgtgt
     2881 tgtgcgtcgc agcagcgcct gac
//
LOCUS       DASWZT010000147         2193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_54361, whole genome shotgun sequence.
ACCESSION   DASWZT010000147 DASWZT010000000
VERSION     DASWZT010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54361"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VGE92_04015"
     CDS             complement(<1..1005)
                     /locus_tag="VGE92_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313014.1"
                     /translation="MAAARDSHKLPTAVADMIGPVLARLSALAPRPASLEACGLTPEM
                     LANLMLKHLLRAGSLTGGGLSERLGLIGTVLEPIIQFLRQEGQIQLQSRASFDNEVRL
                     TLTERGRQGAEDALRRCGYVGPAPVPLETSTRLVSEQTVPRRSLGRELVTRAFRDIIM
                     SESLCERLGVAMASGRAIFIYGPAGAGKTYIASRLIAALTGEILIPHAITVNGKIVRV
                     FDAAVHDRIELLESNRRLLLAQGYDARFALCERPLIIVGGEMTEDMLDVQFNSVTHAY
                     DAPLQMKANGGLLVIDDLGRQRFMPQRLFDRWIVPLEQQVDHLAAGVDARFATPCDAI
                     I"
     gene            complement(1005..1550)
                     /locus_tag="VGE92_04020"
     CDS             complement(1005..1550)
                     /locus_tag="VGE92_04020"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEY1313015.1"
                     /translation="MHSTLAWCLFTLMLAAAVYMDVTRHRIPNKLNLIGLCTALVLQG
                     VTGGVAGLTAGLLGGLAGFGCFAPLYVLRGMGAGDVKLLAAVGTFLGPLGAAYAAMFS
                     LVAGGLGAIGYVVWRAVRAGVNGWAHEGSSAIGASAFVAVRLARRDRLPFAVPIAAGS
                     LSAAAYSVHLTGLARWLQSAI"
     gene            complement(1585..1773)
                     /locus_tag="VGE92_04025"
     CDS             complement(1585..1773)
                     /locus_tag="VGE92_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313016.1"
                     /translation="MNIDGDDHERDGSSVLADEDGLKMVEYAVAGALVITGAVAAFSD
                     LGSAFVTDITFPTTAVSS"
     gene            complement(1854..2021)
                     /locus_tag="VGE92_04030"
     CDS             complement(1854..2021)
                     /locus_tag="VGE92_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEY1313017.1"
                     /translation="MKQFMWDFLKNEEGLTMVEYAIAGALVAGTAVLVFTNLGTAIVA
                     AITKLTTAVNS"
BASE COUNT          480 a          725 c          613 g          375 t
ORIGIN      
        1 gatgatcgca tcgcatggcg tcgcgaaacg cgcgtcgacg cccgccgcca agtgatcgac
       61 ctgttgctcg agcggcacga tccagcgatc gaacagacgc tgtggcatga agcgctgcct
      121 tcccaaatcg tcgatcacca acaggccgcc gttggccttc atttgcagcg gtgcgtcgta
      181 cgcatgagta acggagttga actgaacgtc cagcatgtcc tcggtcattt cgccgccgac
      241 gatgatcagc ggtcgctcgc acagtgcaaa gcgcgcgtcg tatccctgcg ccagcaacag
      301 gcgccgattg ctttcaagca attcgatgcg gtcgtgcacg gccgcatcga agacacgcac
      361 tatcttgccg ttgaccgtga tggcatgcgg aatcagaatc tcgccggtga gggcggcgat
      421 gagccgcgat gcgatgtatg tcttgccggc gccggccggg ccgtaaataa agatggcgcg
      481 cccggatgcc atagccaccc caagacgctc gcaaagcgac tcggacatga taatgtcgcg
      541 aaacgctcgt gtgaccagct cgcgaccgag actgcgacgc ggcaccgtct gttcgctcac
      601 cagccgggtg ctggtctcaa gcggaaccgg agccggtccc acgtacccgc aacgccgcag
      661 cgcatcctca gcaccctgcc tacctcgctc ggtcagcgtc aggcgcactt cgttatcgaa
      721 actcgcgcgc gattgaagct gtatttggcc ctcttgacgc agaaattgga tgatgggctc
      781 gagcacggtg ccgatcaagc cgagacgctc gctcaagcct ccgccggtca atgaaccggc
      841 gcgaagcaga tgcttcagca tcaggttcgc cagcatttcc ggtgtgagcc cacacgcctc
      901 gagacttgcg ggacggggag cgagcgccga cagccgggcc agcaccggtc cgatcatgtc
      961 ggcgacggca gtcggtaact tgtgtgagtc tcgtgccgcc gccattagat agcgctctgc
     1021 agccatcgtg ctaaacccgt caagtgcacg gagtacgccg ccgccgagag actgccagca
     1081 gcgatgggca cagcaaacgg cagtcgatcg cgccgcgcga gtcgaacggc cacgaaggcc
     1141 gacgcgccga tggccgaaga tccttcgtgg gcccagccat tgacgcccgc acgcaccgcc
     1201 cgccagacca catagccgat ggcgccgaga ccgccggcga ccagagaaaa catggccgcg
     1261 taagctgcgc ccagcggacc caaaaaggtg cccaccgcgg ccaataactt gacatcgccc
     1321 gcacccattc cgcgaagaac atagagcggt gcgaaacagc caaaacccgc caaacccccg
     1381 agcaagccgg cggtcaatcc cgcgacccca cccgtcacgc cctgcagcac gagagccgtg
     1441 cacagaccga ttaaattgag cttgttgggt atgcggtgac gagtgacatc catgtagacg
     1501 gcggcagcca acatcagagt gaacaaacac cacgcgagcg tgctatgcat caggccctgc
     1561 ttgcgtttgc aaacgcggca tgagtcaact actaaccgcg gtggtgggga aggtgatgtc
     1621 ggtcacgaac gccgacccca aatcggaaaa cgcagccacc gcgccggtga tcaccaaagc
     1681 gccagcgact gcgtactcca ccattttcaa tccgtcctca tcggcgagga cgctgctacc
     1741 atctctttca tgatcatccc catcgatatt cattcgccca atcgcatcct cactcaagct
     1801 cgatcggaag ccgcgagccg agtagatctc acggcgccta ccgaggctgc tcctcaagaa
     1861 tttaccgcgg tcgtcagttt ggtgatcgcc gcgacaatgg ccgtacccaa attagtaaat
     1921 acaaggaccg cggtgccggc caccaaagcc ccggcgatgg catattccac catcgtcagc
     1981 ccttcctcgt tcttcagaaa atcccacatg aattgcttca ttcgattagc tcctcgtagc
     2041 gttccggttc ggtcctcaag aatttaccgc ggtcgtcagc ttggtgatcg ccgcgacaat
     2101 ggcggtaccc aaattagtaa atacaaggac cgcggtgccg gccaccaaag ccccggcgat
     2161 ggcatattcc accatcgtca gcccttcctc gtt
//
LOCUS       DASWZT010000148         1580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_30226, whole genome shotgun sequence.
ACCESSION   DASWZT010000148 DASWZT010000000
VERSION     DASWZT010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30226"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="VGE92_04035"
     CDS             <1..1024
                     /locus_tag="VGE92_04035"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="archaeosortase/exosortase family protein"
                     /protein_id="HEY1313018.1"
                     /translation="YQRESRRERANQAAALIIPKASRFTNCMAAAPNPTFMHSAGELW
                     RRPAPTQFRISARLLLWFLAIVIDFFFAPALYNGSTIWMSATAMLLIYRAGQAPGAGR
                     ISIDISPAAARLALFVALNTAIVLAARYFAAPLEAAAASDALDAAARAAIKFSVLLPV
                     IALFPLSTWRDVLRTFRAEWTAATVVLLTFFPYRLFRMIFPLYSQVIGKLAYWMARPF
                     VAGTHYLGGPAPIIVAPQLSLQIVFACSGLSALILFDLIVALIAFLDWNSLHRARLFA
                     AYVFGSAAILAANILRIGLLVIIGNRIAPAYATGEFHVNAGWLFFAAVYTCIVVGSYR
                     WMLQQP"
     gene            complement(1093..>1580)
                     /locus_tag="VGE92_04040"
     CDS             complement(1093..>1580)
                     /locus_tag="VGE92_04040"
                     /inference="COORDINATES: protein motif:HMM:NF024731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1313019.1"
                     /translation="PCPCGFFGDPTRQCQCSPPQIQRYVSRISGPLLDRIDIQIEVPA
                     VKYKELRGTSEVECSCAVRERVIRARGVQMARYAGEKMIYANAQMPPNLLRKYCAISA
                     ESETQLEMAIQRMGLSARAHDRILKVARTIADLDGAESIASKHVGEAIQYRTLDRTYW
                     S"
BASE COUNT          255 a          561 c          434 g          330 t
ORIGIN      
        1 ataccaacgc gaatcacgcc gcgagagggc gaatcaggca gcggcgctca taatcccaaa
       61 ggcgtcgcgg ttcacgaatt gcatggcagc ggcacccaac ccgaccttca tgcactccgc
      121 cggcgaactg tggcgccgcc ccgcacccac gcaatttcgc atcagcgcgc gcctgctcct
      181 ctggttcctc gcgatcgtaa tcgacttctt cttcgcgccc gccctttata acggctcgac
      241 catctggatg tcggccaccg ctatgctgct gatctaccgg gcaggtcagg cgccgggagc
      301 cgggcgcatc tcgatcgaca tttcgccagc agcggcgcgc ctcgcgctct tcgtcgcgct
      361 gaatacggcc atcgttctgg cggctcgtta tttcgccgcg ccgcttgaag ccgcggcggc
      421 cagcgatgca ctcgacgccg ccgcccgcgc cgctattaaa ttcagcgtcc tgcttcccgt
      481 gatcgctcta ttcccgttgt cgacctggcg cgacgtgctg cgaacgttcc gcgcggagtg
      541 gaccgccgcc accgtcgtcc tcttgacatt tttcccctac cgcctgttcc gcatgatttt
      601 tcccctctat tcgcaggtga tcggcaagct ggcttactgg atggcgcggc ctttcgtcgc
      661 cggaacgcac tatctgggtg gtcccgcgcc gatcatcgtg gcgccacaac ttagcctgca
      721 aattgtgttc gcctgctcgg gcctctcggc gctcatcctg ttcgacttga tcgtcgcgct
      781 tatcgccttc ctcgactgga actcgctgca ccgcgcgcgc ctctttgccg cctatgtgtt
      841 cggcagcgcg gcgattttag ccgcgaatat cctgcgcatc ggcctgctgg tgatcatcgg
      901 aaaccgcatc gcgccggcct acgcgacggg cgaatttcat gtgaacgccg gatggctctt
      961 cttcgccgcg gtatacacat gcatcgtggt cggctcctac cgctggatgc tccagcagcc
     1021 gtaggggcgg gtctcaagtt ccgcccgctc cggcgtttgt cggatggcgc agcaatatcg
     1081 tcccggccag cctcatgacc aatacgtccg atcgagcgtg cggtactgaa tagcttcgcc
     1141 gacgtgcttc gacgcaatgc tctccgcgcc atccagatcg gcaatcgtcc gcgcgacttt
     1201 caaaatccgg tcatgagcgc gcgccgacaa tcccatccgc tgaatcgcca tctcgagctg
     1261 cgtctcgctc tcggcggaaa tcgcgcaata cttccggagc aagttcggag gcatctgcgc
     1321 gttggcgtaa atcatcttct cgcccgcgta ccgcgccatc tgcacgcctc gcgcgcgaat
     1381 cacccgctcg cgcacggcac acgagcactc cacctcgctc gtgccgcgca gttccttata
     1441 cttcaccgcc ggaacttcaa tctgaatatc gatgcgatcg agcagcggcc cggaaatcct
     1501 cgacacatac cgctgaatct gcggagggct gcactggcac tgccgcgtcg ggtccccgaa
     1561 gaatccgcac gggcacggat
//
LOCUS       DASWZT010000149         6030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_10809, whole genome shotgun sequence.
ACCESSION   DASWZT010000149 DASWZT010000000
VERSION     DASWZT010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6030
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10809"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VGE92_04045"
     CDS             <1..493
                     /locus_tag="VGE92_04045"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016144233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEY1313020.1"
                     /translation="NVGIVLTRGAGELFLGGRVGGRGWQFPQGGVNRGERVEEALFRE
                     LKEEIGLERDDVEVLGSTQGWLRYRLPRQYVRDRCIGQKQRWFLLKLIADESKLRFDS
                     TDTPEFDRWRWADYWTPVREVVYFKRRVYVRALHELGKVMFPQGLPPYPEWWPEIEAA
                     AAR"
     gene            complement(512..928)
                     /gene="hemJ"
                     /locus_tag="VGE92_04050"
     CDS             complement(512..928)
                     /gene="hemJ"
                     /locus_tag="VGE92_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019650354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HEY1313021.1"
                     /translation="MLWLKAFHIVAVVAWFAGLFYLPRLYVYHADAVDSISIERFGIM
                     ERRLFGIMTIGAVATLVLGGTMLWAAPTYLGMQWLRIKLVLVVLLIAYHLCCYKLMRD
                     FAQRRNTHSAKWYRAFNELPSLLLIAIVLLAVVKPL"
     gene            1024..1818
                     /locus_tag="VGE92_04055"
     CDS             1024..1818
                     /locus_tag="VGE92_04055"
                     /inference="COORDINATES: protein motif:HMM:NF042896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HEY1313022.1"
                     /translation="MASLGKLVVRSHTPRRQALLLGAALVGAMLILYAAFEIGRYDAG
                     FRVVDSVRGALAASSRIRDLEAENEKQRNQLEAAEVARRVDREGYKQIERSLGDMQSQ
                     IARLNQDLSFYRGLVQPDSITHVKVQQMQIVPEPTAGQYRLKFVLMQTGKPDSVVAGN
                     AMINIDGLLRGKPSSMSFGQVAPNHRVSLAYSFRYFQDYDEPIHLPQGFEPTRVGVEI
                     RSGRDKDASHGFRQAFVWKAQGMSVETEASGTPAAKGDIDVQTETE"
     gene            1796..2203
                     /locus_tag="VGE92_04060"
     CDS             1796..2203
                     /locus_tag="VGE92_04060"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY1313023.1"
                     /translation="MFKQKQNKSARIDTLIGPETRINGDVAFAGGLHLDGYINGNVRS
                     ELAAGSFLSVSEQGCIDGSVVASHIILNGIVKGDIEACERVELGPKARVLGNVQYSII
                     ETAVGAQINGKLIHRTNSGASGGEPGPKDSPEL"
     gene            2278..2619
                     /gene="erpA"
                     /locus_tag="VGE92_04065"
     CDS             2278..2619
                     /gene="erpA"
                     /locus_tag="VGE92_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009408529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEY1313024.1"
                     /translation="MPPPPNVLVFTDAAAGKVGELIREEANPNLKLRVFVSGGGCSGF
                     QYGFTFDEKIEDGDFCVENHGVQLLVDPMSAQYLMGAEIDYKEDLQGAQFVIRNPNAK
                     TTCGCGSSFSA"
     gene            2637..4139
                     /gene="ppx"
                     /locus_tag="VGE92_04070"
     CDS             2637..4139
                     /gene="ppx"
                     /locus_tag="VGE92_04070"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR03706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEY1313025.1"
                     /translation="MSGGRVVPDVLAAVDLGSNSFHMVVARHSDGELIILDRLREMVR
                     LAAGLGDSGRLDDSATDRALRCLERFGQRLRAMHADSVRVVGTNALRRAKRKRWFLER
                     ARAALGHPIEIISGLEEARLIYSGVVHTSPMSPDKRLVIDIGGGSTEVVIGEGFNPLL
                     LESLSVGCVGLSAIFFDDGKISEKRFERARTAVRLELEPIQEAYRKLGWLQAFGSSGT
                     VRVIGDVLRSLNPEAPRITLENLRLLAERVIAAGHVDELDLEDVDAERAPVFPAGLAI
                     LLEIVENFTIDRVRVAEGAMREGLLYDLMGRFTDEDARVRSVRGMEKRYHVDGAQADR
                     VETTAVGLLEQVESEWGLEDPLAESVLRWAARLHETGLDIAHSKYQRHSAYLLQNADM
                     PGFPREEQLLLSALVGGHRRQLSAESLEDLLPPWDRLAEFLIVLLRLAVLLHRGRSPQ
                     PLPEVNLTVKGRSVNLKLPQGWMKEHPLTLEDLEQERVYLKDAGFRLNIG"
     gene            complement(4147..>6030)
                     /gene="ppk1"
                     /locus_tag="VGE92_04075"
     CDS             complement(4147..>6030)
                     /gene="ppk1"
                     /locus_tag="VGE92_04075"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003918.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEY1313026.1"
                     /translation="LLEAGAVQGGPDGLAVPDQLKAIRARAVRLVDEQYQLLNDVLMP
                     ELAQKGVVFVSPEMWTEAQAAWLADYFSREVEPVLSPLALDPARPFPQILNKSLNFAV
                     VVAGEDGFGRNSGLAVVQAPRSLPRLIRLPDELGSRNFVFLGTIVEAFVSRLFAGMQM
                     RGCYQFRVTRNSDLFVEQEEVDDLLRAVEGELASRRFGDAVRLETAHDCPEEILSYLL
                     AQFGLSGEDLYKVSGPVNLNRLMAVYDLIDRTELKYAPFAPSVPERLRMGSDLFATLR
                     AGDVLLHHPFQGFGPVTDFIKQAASDPQVLAIKQTLYRAGSDSTIVGALAEAAQAGKD
                     VTVIIELRARFDEEANIELATKLQEAGAHVMYGVFGFKTHAKLVMVVRREEKGLRRYC
                     HLGTGNYHPKTARLYTDYGLLTADESIGEDIHEIFLQLTGLTRVPRLRKLLQAPFGLH
                     QALLDKIERESVHARAGKPARIIAKLNALTEPVIIQALYRASRAGVNVDLIVRGVCCL
                     RPGVPGVSERIQVRSIVGRFLEHSRVYYFENAGEREVYCGSADWMDRNLFRRIEVAFP
                     VEAPHLASRVADDLKLYLADDMQAWVLNGTGHYARTFGSDSTSAQARLLGLYDERIPL
                     TDG"
BASE COUNT         1186 a         1780 c         1946 g         1118 t
ORIGIN      
        1 caacgttggc atcgtgctga cccgcggcgc cggagagctg ttcctcggag gccgggtggg
       61 cggccgcggc tggcaatttc cccaaggcgg cgtaaatcgc ggcgagcgcg tcgaagaggc
      121 gctgtttcgc gagctcaagg aggaaatcgg cttggaacgc gacgacgtcg aggtcctggg
      181 ctcgactcaa gggtggttgc gctatcgatt gccgcggcag tacgtacgcg atcgctgtat
      241 cggtcagaag cagcgctggt ttctgctcaa gctgatcgcc gacgaatcca aactgcgatt
      301 cgattccacg gatacgcccg agttcgatcg ttggcgctgg gccgactatt ggaccccggt
      361 gcgcgaggtg gtttatttca agcggcgggt gtatgtgcgc gccctgcatg agctcggcaa
      421 ggtgatgttt ccgcaggggc tgccgccata cccggagtgg tggccggaaa tcgaggcggc
      481 ggccgcgcgc tgatgcgcga tcgaagctcc cctaaagcgg tttgaccacg gccagaagta
      541 cgatggcaat gagcaacagc gacggcagtt cattgaaggc gcgataccat ttggcgctgt
      601 gggtgttgcg tcgctgcgcg aaatcgcgca tcaatttgta gcagcacaga tggtaggcga
      661 tcaataacac caccagcacg agtttgatcc gcaaccactg catcccgagg taagtgggtg
      721 ccgcccacaa catcgtcccg cccaaaacca aggtggcaac cgcaccaatg gtcataatgc
      781 cgaataaacg ccgctccatg atgccgaagc gctcgatgct gatcgagtcc acggcgtcgg
      841 cgtgatagac gtagaggcgc ggtaaataga ataggccggc gaaccacgca accaccgcca
      901 cgatgtgaaa tgctttgagc cagagcatga gcgttccttc gattctgcat cctataccgt
      961 ggtgcgaaac ctgtcgcttc gtgtaatgtg ccggtcggat aacggcgcgg gttcgggggt
     1021 gcggtggctt cactcggcaa attggtcgtc agatcgcata cgccgcgacg tcaagccttg
     1081 ctgctgggcg cggcgctggt cggggccatg ttgatcctct acgcggcatt cgaaatcggc
     1141 cgctacgacg cgggctttcg ggtggtcgat tcggtgcgtg gtgcgttggc ggcgagttcg
     1201 cgcatccgcg acctcgaagc ggaaaatgaa aagcaacgca atcaactgga agccgccgaa
     1261 gtcgcgcgcc gcgtcgatcg cgagggctac aaacagatcg aacgcagtct cggcgatatg
     1321 cagagtcaaa ttgcccgcct gaatcaagac ctatcgttct atcgggggct ggtgcagccg
     1381 gactcgatca cacatgtcaa agtacagcag atgcagatcg tgccggaacc caccgccggt
     1441 cagtaccgat tgaaattcgt gttgatgcag accggaaagc ctgacagcgt ggtggcgggc
     1501 aatgccatga tcaacatcga tgggctgctg cgcggcaaac cctcgagcat gagttttggc
     1561 caagtcgcgc ctaaccaccg tgtcagcctg gcatactcgt tccgctactt tcaggattac
     1621 gacgagccga tccacttgcc gcaggggttc gaaccgacgc gcgtcggcgt cgagattcgc
     1681 agcggcagag ataaagacgc gagtcacggt tttcgacagg ccttcgtgtg gaaagcgcaa
     1741 ggaatgtccg tagaaaccga ggcgagcggc acgccggcag cgaagggtga catcgatgtt
     1801 caaacagaaa cagaataaaa gcgcgagaat cgataccttg atcggtcccg agacacgaat
     1861 caacggcgac gttgcgtttg ccggcggcct gcatcttgac ggctacatca acgggaatgt
     1921 caggagcgag ctcgcggcgg gtagcttctt gtcggtcagc gaacagggtt gcatcgacgg
     1981 ctcggtggtg gcctcgcaca tcatcctcaa cggcatcgtc aaaggcgata tcgaggcctg
     2041 cgagcgggtg gagctggggc caaaagcgcg cgttttgggc aatgttcaat acagcatcat
     2101 tgaaaccgcg gttggcgccc aaatcaatgg taagttgatc catcggacga attcgggggc
     2161 cagtgggggt gaacccggtc ccaaagacag tcctgaactt tgatcggcca cgcgccagca
     2221 gggtagaata gatccatgag tgctacacac gaatcgatgc ctgcgggcgg ggaggccatg
     2281 ccgcccccgc cgaacgtgct ggttttcacc gacgctgcgg ccggcaaagt gggggagttg
     2341 attcgcgaag aggcgaaccc caacctcaaa ttacgggtat tcgtctccgg cggcggttgt
     2401 tcggggtttc agtacggttt caccttcgat gagaagatcg aagacggcga tttctgcgtc
     2461 gagaatcacg gcgtgcagtt gctggtcgat ccgatgagcg cgcagtacct catgggtgct
     2521 gaaatcgact acaaagaaga tttgcagggc gctcagttcg tcattcgcaa cccgaacgcg
     2581 aaaacaacct gcggttgcgg gtcttcgttc tcagcctaag aatgttgacc gcgtagatga
     2641 gcggcggtcg tgtcgtgccc gacgtgctcg cggccgtcga cctggggtcc aacagctttc
     2701 acatggtcgt ggcgcgtcat tcggacggcg agctcatcat tctggatcgg cttcgcgaga
     2761 tggtgcgcct cgccgcagga ttgggcgaca gcggccggct cgacgattcc gcgactgacc
     2821 gcgcactgcg ctgcctcgag cgtttcgggc aacggctgcg cgcgatgcac gccgacagcg
     2881 tgcgggtcgt cggcaccaac gccctgcggc gcgccaagcg caagcgctgg ttcttggaac
     2941 gtgcgcgcgc cgcgctcggt catccgatcg agatcatctc cggactcgaa gaggcgcggc
     3001 tgatttattc cggagtcgtg cacacgagcc cgatgagccc cgacaagcgg ctggtgatcg
     3061 atatcggcgg cggatcgacc gaggtcgtga tcggcgaggg attcaatccg ctgctgctcg
     3121 agagtctgtc ggtgggctgc gtcggattga gcgccatctt tttcgatgac ggcaagatct
     3181 ccgagaaacg cttcgaacgc gcgcgcaccg ccgtgcgact cgagctcgag cccatccaag
     3241 aggcgtaccg caagctcggt tggctgcagg cattcggcag ctccggcacg gtgcgcgtca
     3301 tcggcgatgt gctgcgcagc ctcaatcccg aagcgccgcg catcacgctc gagaatctgc
     3361 gtctactcgc ggagcgggtg atcgccgcgg gtcatgtgga tgaactcgat ctcgaggatg
     3421 tggatgcgga gcgcgcaccg gtgttccccg ccgggctggc gattctcttg gaaatcgtcg
     3481 agaacttcac catcgaccgc gttcgcgttg ccgaaggtgc gatgcgcgaa ggattgctct
     3541 acgacctgat gggccgcttc accgacgagg acgcgcgcgt gcgcagcgtg cgcggcatgg
     3601 aaaagcgcta ccacgtcgac ggcgcacagg cggatcgcgt cgaaaccacc gcggtcggcc
     3661 tgctcgagca agtcgagtcc gaatggggat tggaggaccc gcttgcggag tccgtgctgc
     3721 gctgggccgc gcggcttcac gagacgggac tcgatatcgc gcattccaag taccagcggc
     3781 atagcgccta tctgctgcag aacgccgata tgcccggctt tcctcgcgaa gagcagctgt
     3841 tgttgtccgc gttggtcggc ggccatcgcc ggcagctgtc cgccgaatcc ctggaagact
     3901 tgctgccgcc ctgggatcgg ctcgcggagt ttctgatcgt gctgctgagg ctcgcggtgc
     3961 tgctgcaccg aggccgtagt ccgcaaccgc ttccggaagt gaatctcacg gtcaagggcc
     4021 gcagcgtgaa tctgaagctg ccgcaaggct ggatgaagga acacccgctc acattggagg
     4081 atctggaaca ggagcgggtg tatttgaagg acgccggatt tcgcttgaat atcggctgaa
     4141 ttgctctcaa ccgtccgtga gcgggatccg ttcgtcatac agacccaaga gacgcgcctg
     4201 agcgctggtg ctgtcgctgc cgaaggttcg ggcatagtgg cccgtgccat tcagaaccca
     4261 agcctgcata tcgtcggcca gatacaactt gaggtcgtcg gcgacgcggg aggcgaggtg
     4321 gggcgcttcg acggggaatg cgacctcgat gcggcggaac aaattacggt ccatccaatc
     4381 ggcgctgccg cagtaaactt ctcgctcgcc ggcattctcg aagtaataca cgcgcgagtg
     4441 ctcgaggaag cggccgacga tggagcgcac ctggatacgt tcggaaacac ccgggacgcc
     4501 cggccgcagg caacacactc cgcgtacgat gagatccacg ttcacgccgg cgcgcgaggc
     4561 tcgatacagc gcctggatga tgacgggttc ggtcaacgca ttgagcttgg caatgatgcg
     4621 ggcaggcttg cccgcccgcg catgcacgct ctcgcgttcg atcttgtcga gcagcgcttg
     4681 atgcaggccg aagggcgcct gcaggagctt gcgcagccgc ggcacgcgcg taagtccggt
     4741 caattgcaga aaaatttcgt ggatgtcctc gccgatcgac tcatcggccg tcaacaggcc
     4801 gtagtcggtg tacaggcgcg cggttttcgg atgatagttg ccggtgccta ggtggcagta
     4861 gcggcgcagc cccttctcct cgcgccgcac caccatgacg agcttggcgt gtgtcttgaa
     4921 accgaacacg ccgtacatga cgtgggcgcc ggcttcctgc agtttggtcg caagttcgat
     4981 gttcgcctct tcatcgaaac gcgcgcgcag ctcgatgatg acggtgacgt ctttgcctgc
     5041 ctgtgcagcc tcggcgagcg cacccacgat cgtggagtcg ctgccggcgc gatatagggt
     5101 ctgtttgatg gcaagcacct gcgggtcgct cgccgcctgc ttgatgaaat cggtgacggg
     5161 cccaaatcct tgaaaaggat gatgcagaag cacgtcgccg gcgcgcagcg tcgcaaacag
     5221 atcgctgccc atgcgcagtc gttccggcac gctcggcgcg aacggcgcgt acttgagttc
     5281 ggtgcggtct atcagatcgt agacggccat gagccgattc agattcaccg gtccggacac
     5341 tttatagagg tcttcgccgc tcagtccaaa ttgcgccaac aggtagctga gaatctcctc
     5401 ggggcaatcg tgggcggttt ccagccgcac tgcgtcgccg aagcggcgcg aggcgagttc
     5461 gccttcgacg gcgcgcagca ggtcgtcgac ttcttcctgc tcgacgaaca aatcgctgtt
     5521 gcgcgtgacg cgaaattgat agcagccgcg catctgcatg cctgcgaaca gcctggagac
     5581 gaaggcctcg acaatcgttc ccaagaaaac gaagttgcgg ctgccgagct cgtccggcag
     5641 gcgaatcagg cgcggcaaag aacgcggcgc ctgcaccacg gcgagaccgc tgttgcggcc
     5701 gaagccatcc tcgcccgcga ccaccaccgc gaaattcagg ctcttgttga ggatttgcgg
     5761 gaacgggcgc gcaggatcca gagccaacgg gctcagcacc ggttcgactt cgcggctgaa
     5821 ataatcggca agccatgcgg cctgcgcctc ggtccacatc tcgggcgaca cgaacaccac
     5881 acccttctgc gcgagttccg gcatgagcac gtcgttcagc agctggtatt gctcgtcgac
     5941 caaacgaacg gcgcgggcgc gaatcgcttt cagctgatcc ggcaccgcca gtccgtcggg
     6001 acccccctgc accgcgccgg cctccaacag
//
LOCUS       DASWZT010000150         6831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_34970, whole genome shotgun sequence.
ACCESSION   DASWZT010000150 DASWZT010000000
VERSION     DASWZT010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6831
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34970"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1209)
                     /gene="proB"
                     /locus_tag="VGE92_04080"
     CDS             complement(46..1209)
                     /gene="proB"
                     /locus_tag="VGE92_04080"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEY1313027.1"
                     /translation="MTVADRKLVARRLAEAKRAVVKVGSTLLVDAEKGRLNRAWLESF
                     AQDVAAMRKRGQDVILVSSGAIALGRRHLGLNAGKLRLEESQAAAAVGQIRLAHAYKE
                     LLDAHDITVAQVLLTLGDTEQRRRYLNARGTLNTLLSLGAVPVINENDTVATTEIRYG
                     DNDRLAARVAQMSGADCLVLLSDIDGLYTSDPTLDPQAEFIGRVLEITPAIEAMAGGA
                     ASDMGSGGMHTKIAAAKIAVAAGCHLCIAKGAVQHPLQRIEEGARCTWFVPSSTPMAT
                     RKQWIAGTLRPAGAIVVDDGAVRALLGGKSLLPAGVTRAVGRFDRGDTVSILGPDGSE
                     VARGICAYSDGDAARIIGRQSAEIEQVLGFRGRDEIVHRDDLVLLRAHVVGTL"
     gene            1298..1621
                     /locus_tag="VGE92_04085"
     CDS             1298..1621
                     /locus_tag="VGE92_04085"
                     /inference="COORDINATES: protein motif:HMM:NF042799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6763 family protein"
                     /protein_id="HEY1313028.1"
                     /translation="MGREYEPTVGQWYEDLENEETFQVLKVDEDKETVEIQHLDGDIE
                     EFDVDGWAELDLELTEEPEGWSGSKAEKDADDDEEEDEDWDEDDDDDDEDDDLDDEDE
                     DREEY"
     gene            complement(1618..2634)
                     /locus_tag="VGE92_04090"
     CDS             complement(1618..2634)
                     /locus_tag="VGE92_04090"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:TIGR01123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEY1313029.1"
                     /translation="MTAQPQRPAFGTLLTSHMAVATYQAGRWSPSEIKPVAPIELSPA
                     AHVLHYASTCFEGFKAFRRADGSVHIFRMDRHIQRMRQSARQLVLPEPDAIQLADMVR
                     AVINRCRDAVPEAPGALYLRPILFGTTANIGAAATPAAEASLLVLASPVWDYFSGGMK
                     PLRILVDDENTRSAAQMGMVKTGGNYAAALGPTLSARVKYQVDQVLFCPGGQVQETGA
                     ANFVLIRDRELLTRSLDSTFLHGVTRDSLLTIARDLGYKVSERRFEVQEMLEWVKTGE
                     AALSGTAAVLAGVGTLIYRGTEYPVAGGDVGPLTRALRAQLIAVQQGETADRHGWLER
                     VEEV"
     gene            complement(2631..3869)
                     /locus_tag="VGE92_04095"
     CDS             complement(2631..3869)
                     /locus_tag="VGE92_04095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEY1313030.1"
                     /translation="MSHYVFKMPDLGEGTVDAEIVAWHTKPGDAIVEDQLIVEVMTDK
                     AAVEVPAPVSGRVISITGAPGDKVAVGSPLIVFDTGAAASDGAQLTPSPAADSADPAA
                     APPLVAAPAGALAPAANAPHPGRVMTSPANRRRAREAGIDLSTVVGSGPGGRILREDL
                     GTIAAAAPNAGSSGAAETSEIKVIGLRRLIAERMSEAKRNIPHFAYVEEVDVTELESL
                     RNHLNASRPSGTGSLSYLPLVVTALTRVLESFPQCNVLYDAARGVLVRHRAVHVGIAT
                     QTPDGLKVPVVRNAGSLGLWELAAEIRRLAERARTAKSARDELVGSTITVTSLGKLGG
                     IASTPIINAPEVAIIGLNKAVERPVILNGAVAVRRIMNLSSSFDHRFVDGYDAAAMIQ
                     ALKELLEHPATIFIPKVQSK"
     gene            complement(3973..4989)
                     /locus_tag="VGE92_04100"
     CDS             complement(3973..4989)
                     /locus_tag="VGE92_04100"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY1313031.1"
                     /translation="MANMNMIQALNSAMDIMLKQDASVVLMGEDIGYFGGVFRATEGL
                     QRKYGEHRVIDTPIAEGGIVAAAIGMSVNGMRPVAEIQFADYIYPGFDQIASELARIR
                     YRSAGEFFAPVTIRTPCGGGIRGGQTHSQSPEGIFTHVSGIKVVMPSNPYDAKGLLIT
                     SIEDDDPVIFFEPKRLYNGPFDGDPSKPAVPWSSHPKGQVDEGYYKVPLGKADIVRPG
                     AQVSIITYGTMVFVAAAAAAAMNLDAEIIDLRSLVPLDVETICNSVRKTGRCVVAHEA
                     TRFSGFGAEILSVVQEECFWDLEAPIQRVTGWDTPYPHAFEWEYFPGQARVAMALKAV
                     MEPV"
     gene            complement(4992..6248)
                     /locus_tag="VGE92_04105"
     CDS             complement(4992..6248)
                     /locus_tag="VGE92_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY1313032.1"
                     /translation="MAMEKRSRLHLPSPPARPGEAPDFSYVQLSPAGAVARPDINARA
                     RDIGSLSLDLVRVLDDAGKAVGPWHPHLDPAELQVGLRHMLLTRLFDDRMQRTQRAGK
                     ISFYIRSMGEEAVSVAQCMALRPGDMLFPSYRNQGLQIARGITLVDLMCQLLSNTRDM
                     CKGRQLPVMYHSKAGNIFSISGNLATQFPQAVGWAMAAAIKGEDHLAASWIGEGSSAE
                     ADFHHALLFASVYQAPVILNVVNNQWAISTFQGYAGGEQRSFAARGPGYGIAGVRVDG
                     NDFLAVYAVTQWAAERARTGAGPTLIELVTYRAAAHSTSDDPARYRAKNDHEHWPLGD
                     PIVRLKQHLIGLGEWSEERHQSLTAELETLVASSWKEATSFGTMSEGPRLDADLMFED
                     VFKEMPEHLQKQRALMRAERSRAARS"
     gene            6335..6817
                     /locus_tag="VGE92_04110"
     CDS             6335..6817
                     /locus_tag="VGE92_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010207358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY1313033.1"
                     /translation="MENLSRPAIDRLDLRLLDILQRHGALPVAEVAAQTRLSTTTCWR
                     RIQQLEQSGVIKARVALLDRNALGLDVTIFAHVKLATTGRDAIAEFEVAIRDRAEVLE
                     CYTLTGEWDFLLKIVTQNIKAYEAFYLDYLSRIPCVRSVNSSVAVTVIKESTALPLGI
                     "
BASE COUNT         1323 a         2184 c         2043 g         1281 t
ORIGIN      
        1 ccgcctctcc gataccgcgc atcagatcag ccaccggaac ctgcttcata gggtgccgac
       61 cacgtgagcg cgcaacaaca ccaagtcatc gcggtgcacg atttcatccc ggccgcgaaa
      121 ccccaagacc tgctcgatct cggccgattg tcggccgatg atgcgcgccg cgtcgccgtc
      181 ggaatacgcg cagatgccgc gcgccacctc cgagccgtcc ggccctaaga tgctcaccgt
      241 gtcgccgcga tcgaatcgtc ccacggcacg agtcaccccc gcgggtagca agcttttgcc
      301 gcccagcagc gcgcgtaccg ccccgtcgtc gaccacgatc gcgccggcag gtctcaaggt
      361 accggcgatc cactgtttgc gggtcgccat cggcgtcgat gacgggacga accaggtgca
      421 acgcgcaccc tcttcgattc gctgcaaggg atgttgtacc gcccccttgg cgatgcataa
      481 gtgacacccg gcggccaccg ctattttggc ggcggcgatc ttggtgtgca tgcctcccga
      541 acccatgtcg ctggccgcgc cgccggccat ggcctcgatg gccggtgtga tctcaagcac
      601 ccggccgatg aactccgctt gcggatcgag cgtcgggtcg gacgtataca gaccgtcgat
      661 gtcggacagc agaaccaagc agtccgcgcc tgacatttgc gcgacgcgcg ccgccagacg
      721 atcattatcg ccatagcgga tctcggtcgt ggccaccgtg tcgttttcat tgattaccgg
      781 caccgcaccg agcgacagca gggtattgag cgtgccgcgc gcattcagat agcggcgccg
      841 ttgttccgtg tcgcccaagg tcaacaggac ttgcgcgacg gtgatgtcgt gcgcatccag
      901 cagctccttg taggcatgcg ccaggcgtat ttgacccacg gcggcggccg cctgactttc
      961 ttcgagccgc agcttgccgg cattcagccc caagtggcgc ctgcccaagg cgatagcgcc
     1021 cgaggacacc aaaatgacgt cttgaccgcg cttgcgcatg gccgccacgt cttgcgcgaa
     1081 gctctcgagc catgcgcgat tcagccggcc tttttccgca tcgaccagca aggtcgagcc
     1141 gactttcacg accgcgcgtt tggcttcggc caagcgacgc gcgacgagtt tgcggtctgc
     1201 gactgtcatg aacacaatat tgccatttac ttgggcaagc ggcctcccct aatattcgcc
     1261 tgccgaatca gcagcagttc ctcaaagtag gtgaatcgtg ggcagagagt acgaaccgac
     1321 cgtcgggcag tggtatgagg acttggaaaa cgaggaaacc ttccaggtgc tcaaagtcga
     1381 cgaggacaag gaaaccgttg agattcaaca tctcgacggc gatatcgagg aattcgatgt
     1441 cgacggctgg gcggaactcg acctggaact caccgaggag cccgaaggct ggtccggctc
     1501 aaaagccgag aaagacgccg atgacgatga ggaggaagac gaagactggg acgaggacga
     1561 tgacgatgac gatgaggacg acgacctaga cgacgaggat gaggatcggg aggagtacta
     1621 gacctcctcc acgcgctcca gccagccgtg ccgatcggct gtttctccct gctgcaccgc
     1681 tatcagttgc gcgcgcaaag cgcgtgtcaa cggtccgacg tcgccgcccg ccaccgggta
     1741 ctcggtgccc cgatagatga gcgtgcccac ccccgccagc accgccgcgg tacccgacaa
     1801 tgcggcctcg ccggttttca cccattccag catttcttgg acttcaaacc gccgctcgct
     1861 caccttgtat cccaagtcac gcgcaatcgt gagcagcgaa tcgcgggtga cgccgtgcaa
     1921 aaatgttgaa tcgaggctgc gcgtcaacag ttcgcggtcg cggatcaaga cgaagtttgc
     1981 ggcgccggtc tcctgtactt ggccgcccgg acagaacagc acctgatcga cctggtattt
     2041 gacccgcgcg ctcaaggtgg gacctagcgc ggcggcataa ttgccgccgg tcttcaccat
     2101 gcccatctga gccgccgagc gcgtattctc atcatcgacc aggatgcgca gcggcttcat
     2161 gccgccggag aaataatccc agacgggact ggcgagcacc agcagcgaag cttccgcagc
     2221 gggtgtcgcg gcggcaccga tattcgcagt cgtgccgaac aaaatcggcc gcaaatacag
     2281 cgcgccgggc gcttccggga ccgcgtcccg gcaacgattg atcaccgcgc gcaccatgtc
     2341 ggcgagctgg atcgcgtccg gttccggcag caccagctgc cgggcgctct ggcgcatgcg
     2401 ctgaatatgc cgatccatgc gaaagatgtg cacggaccca tcggcgcggc ggaacgcctt
     2461 gaagccttcg aaacacgtgc tggcgtagtg cagcacatgg gcggccgggg acaattcgat
     2521 cggtgccacg ggcttgatct cgctcggact ccagcgtcct gcctggtacg tggccaccgc
     2581 catatgtgaa gtcaacaacg tgccgaatgc gggtcgttgg ggttgcgcag tcatttggat
     2641 tgcaccttgg gaataaaaat cgtcgccgga tgttcgagca gctctttgag cgcttgaatc
     2701 atcgccgcag catcataacc gtccacgaag cgatgatcga atgaggaaga caaattcatg
     2761 attcggcgca ccgccacggc gccgttgaga atgaccgggc gctcgacagc cttgttgagt
     2821 ccgatgatcg cgacttccgg cgcgttgatg atgggcgttg aggcgattcc gcccagcttg
     2881 ccgagactgg tgaccgtgat ggtcgatccg accaactcat cgcgcgccga ctttgcggta
     2941 cgggcgcgct cggcgagccg gcgaatctcc gcggcgagct cccataaacc gagggacccg
     3001 gcatttctga cgaccgggac cttcagcccg tccggcgtct gagttgcgat gccgacatgc
     3061 acggcgcggt ggcgcaccag cacgccgcgc gcggcgtcgt acagcacatt gcattgcggg
     3121 aacgactcga gcacgcgggt aagcgccgtc acgaccagcg gcaggtagct caagctcccc
     3181 gtaccggacg gccgcgatgc attcaagtgg ttgcgcagcg actctaactc ggtgacgtcg
     3241 acttcctcga cataagcaaa atgcggaatg ttgcgcttcg cctcgctcat ccgttctgcg
     3301 atcaaacggc gcaagccgat cactttgatt tccgaggttt ccgcagcgcc agacgaaccc
     3361 gcattgggcg ccgccgcggc gatggtcccc aaatcctcgc gcagaatacg gccgccggga
     3421 ccggagccga cgacagtcga cagatcgatg ccggcctcgc gcgcgcgccg acgattcgct
     3481 ggcgaagtca tcactcggcc tgggtgaggg gcattcgccg cgggcgcaag cgctcctgcc
     3541 ggcgcagcca ctaggggcgg cgctgcggca ggatcagccg aatccgctgc gggcgacggc
     3601 gtaagctgcg ccccatccga ggcggctgcg ccggtgtcaa acacaatgag cggcgagccc
     3661 accgccactt tgtcccccgg cgcgcccgtg atcgaaatca cccggccgct cacgggcgcg
     3721 ggcacttcca ccgccgcttt gtcggtcatt acctcgacga tgagttggtc ctcgactatc
     3781 gcgtcgcccg gcttggtatg ccaggcgacg atttccgcat cgaccgtgcc ctcgcccagg
     3841 tcaggcatct tgaatacgta atgactcatc tggcttctcc gcgagcgata ccggccgtca
     3901 agttaaagac tgtatcaatg tacagctccc gtgccgccgc agaatttctg gtccggacag
     3961 gcgaggagga cgtcaaacgg gctccatcac ggctttgagt gccatcgcga ctcgtgcctg
     4021 gcccggaaaa tactcccatt cgaacgcgtg cggataggga gtatcccagc cggtaacacg
     4081 ttggatgggc gcctcgagat cccagaaaca ctcttcttga acgacggaca atatttccgc
     4141 gccaaagccc gagaatcgcg tggcctcatg cgccacgacg caacgtccgg ttttgcgtac
     4201 cgagttgcaa atggtttcaa cgtcgagcgg caccagcgat cggagatcga taatctccgc
     4261 atcgaggttc atggccgccg ccgccgccgc cgcaacgaac accatggtgc cgtaggtaat
     4321 gatgcttact tgtgcgcccg gacgcacgat gtccgctttg ccgagcggaa ctttgtaata
     4381 accttcatcc acctgaccct tcggatgcga actccaagga acggccggtt tgctcggatc
     4441 gccatcaaag ggtccgttgt acagtcgttt gggctcgaag aagatcaccg gatcatcatc
     4501 ctcgatggag gtgatcagca gtcccttggc atcgtaaggg ttcgacggca tcacgacctt
     4561 gatgccgctg acatgggtga aaatgccttc cgggctttgc gaatgcgttt gcccgccgcg
     4621 aatcccgccg ccgcacggag tgcgaatggt caccggcgcg aagaactctc ctgcgctgcg
     4681 ataacggatg cgcgccaatt cgctcgcaat ttggtcgaac ccggggtaga tgtaatccgc
     4741 gaattggatc tcggccaccg gccgcatgcc attgacgctc attccaatcg cggcggcgac
     4801 gatgccgccc tccgcgatgg gggtgtcgat gacgcgatgc tcgccgtact tgcgttgcaa
     4861 tccttcggtg gcgcgaaata cgccgccgaa atagccgatg tcctcgccca tcaacaccac
     4921 cgaagcatcc tgcttgagca tgatgtccat cgcagaattc agggcctgaa tcatattcat
     4981 gttggccatg cttaactcct cgctgcgcgg ctgcgttccg cgcgcatcag ggcgcgttgc
     5041 ttctgtaaat gttcgggcat ttctttgaag acatcctcga acatcagatc ggcatcgagc
     5101 cgcgggccct cgctcatggt cccgaaggag gtcgcctcct tccaactcga ggcgaccagc
     5161 gtttcgagtt cggcggtcag tgactggtga cgctcctccg accactcgcc gagcccgatc
     5221 aaatgttgtt tcaggcgcac gatgggatcg cccagcggcc agtgttcgtg gtcgttcttt
     5281 gcccgatagc gcgccggatc gtcactggtg gagtgtgccg cagcacgata cgtcaccaat
     5341 tcaatcagcg tgggcccggc accggtgcgg gctctctcgg ccgcccattg cgtcaccgca
     5401 tagacggcga gaaagtcgtt gccgtcgact cggacaccgg cgatgccata gcccggaccg
     5461 cgcgccgcaa acgagcgctg ctcaccaccg gcgtaaccct ggaaggtgga gatcgcccac
     5521 tgattgttca ccacattgag aatcacgggc gcctggtaaa cggacgcgaa caataatgcg
     5581 tggtgaaaat cggcctcggc gctgctgccc tctccgatcc agctcgcggc cagatgatct
     5641 tcgcccttga tcgccgccgc catcgcccaa ccgacagcct gcggaaactg agtcgccaaa
     5701 ttgccggaaa ttgaaaagat gtttcctgcc ttggagtggt acatcaccgg caactggcgg
     5761 cctttgcaca tgtcccgcgt attggacaag agctggcaca tgagatcgac cagcgtgatg
     5821 ccgcgcgcga tctgcaagcc ctgattgcgg tacgaaggga acaacatgtc gcccgggcgc
     5881 agcgccatgc attgcgccac cgacaccgct tcttcaccca tcgaacggat gtagaacgaa
     5941 atcttcccgg cgcgctgcgt gcgttgcatc cgatcatcga ataaccgtgt cagcagcatg
     6001 tggcgcagcc ccacttgcaa ctccgccgga tcgagatgcg gatgccacgg tcccaccgcc
     6061 tttccggcat cgtcgagtac ccgtaccaga tcgagcgaca gcgagccgat gtcgcgggcg
     6121 cgcgcgttga tgtccggccg cgcgacggcg ccggcgggcg agagttgcac gtagctgaag
     6181 tcgggagctt caccgggtcg agccggcggg ctcggtaaat gcagtcgcga gcgtttttcc
     6241 atcgccataa tttacgtccc ttgctggctc agagttgcat tcattagggg gtggccggat
     6301 aattcggtat gttcattccg aaatattgca tctagtggaa aatctatctc gccccgcgat
     6361 cgatcggctg gatcttcgtt tgctcgatat tctacagcgc catggcgcgc tgccggttgc
     6421 cgaagtggcc gcccagaccc ggctctccac caccacgtgc tggcggcgca tccaacaact
     6481 ggaacagagc ggcgtgatca aagcacgtgt tgcgctgctc gatcgcaacg cgctggggct
     6541 cgatgtgacc atcttcgcgc acgtcaagct cgcgaccacc ggtcgtgatg cgattgccga
     6601 attcgaagtt gccattcgcg atcgcgccga ggtgctggag tgctatacgc tcacgggcga
     6661 gtgggatttc ctgttgaaga tcgtcaccca gaacatcaaa gcgtatgagg ctttctatct
     6721 cgactatctg tcgcggatcc cgtgcgtacg ctcggtgaac tcatcggttg ccgttaccgt
     6781 catcaaggag tcgaccgccc tgccgttggg gatttagatg cgcgtagcgc g
//
LOCUS       DASWZT010000151         8891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_15616, whole genome shotgun sequence.
ACCESSION   DASWZT010000151 DASWZT010000000
VERSION     DASWZT010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8891
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15616"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1851)
                     /gene="glgX"
                     /locus_tag="VGE92_04115"
     CDS             complement(<1..1851)
                     /gene="glgX"
                     /locus_tag="VGE92_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEY1313034.1"
                     /translation="MKPIQAGLPRPLGAARNKRGVNFAVFSSHATRVEVGLFDPASGA
                     ELARFDLPARSGDIWHGLLSSRRAGTETHYAFYVHGPNQPEKGHRFDATAPLIDPYAR
                     ALSCGWPLRARVVDGSFEWGADRPPAVPWRDTIIYELHVKGFTQLHPAVPAEWRGKYL
                     GLTVPVVIEHLKSIGVTAVELLPCQAFASEQFLRDRRLSNYWGYNSVAWFSAANEYAV
                     RDAVAEFKAMVKALHAARIEVILDVVFNHTAEGNETGPLLSLRGLDNSVYYRLMPQDL
                     RFYENVTGCGNTVNCEHPQVRTLIIDCLKYWVDEMHVDGFRFDLATVLGREGNGFNQH
                     SALFKSLRAEPSLAYVKLIAEPWDVGWGGYQLGRFPAGWSEWNDRYRDAVRAFWRRDA
                     GKLGDLAERFAGSSDIFRHDGRKPTAGINFPTAHDGFTLYDLVSYNQRHNEANLESNA
                     DGHNENLSWNCGAEGPTDDPAVNALRKRQMRNMLATLFLSQGVPMLQAGDEFARTQRG
                     NNNAYCQDNEISWVDWHLKSSHADLLRFVQLLAQLRRRHEEFRRETFLKGATSRASVK
                     DVTWLNVGGAEMKQGEWHDGNLRTLGVWFGKRSNLEGRLLLLLNASDSAQE"
     gene            complement(1851..3758)
                     /gene="glgB"
                     /locus_tag="VGE92_04120"
     CDS             complement(1851..3758)
                     /gene="glgB"
                     /locus_tag="VGE92_04120"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEY1313035.1"
                     /translation="MSDSSLSDFDLYLFGEGKHERIYDKLGAHPYSDAGTSGTRFAVW
                     APNAERVSVVGDFNHWDGGANVMRSRASSGVWELAIPGIGLGSAYKYEIRDRAGGLFV
                     KSDPYGFAMQLRPQNCSLVACLDGYVWGDSQWLAERARRDPLRTPFNAFEVHLGSWRR
                     PWHARSPPFMSWPEAVEQLIPYVVDMGFTHIELMGVAEHPLDASWGYQIVGYYAATAR
                     YGSPHDLMYFIDRCHQAGIGVILDWVPAHFPRDDHGLSYFDGSALYEHADSRLGEHME
                     WGTKIFNYGRHEVRNFLVANALFWFDRYHVDGLRVDAVASMLYLDYSRKPGEWLPNRD
                     GGRENLEAIDFLRQMNNAVHAAFPGVLTIAEESTSFAGVTQPPEYGGLGFNFKWNMGW
                     MNDSLRYVALNPIYRRYNHQLITFSFVYAWSENFILPVSHDEVVHGKRSLLDKMPGDE
                     WQKRANFRLFLAYMCAHPGKKLLFMGSEFGQWQEWRDYEQLEWAQLAVPQHAALRDCV
                     RELNLLYRSTPQFYGSDCDHNGFRWVDLHNADESIWAFSRRAVGTDGGAPITCVFNAT
                     PVPRDNYGVGVADLGSYQKIFDSDDARFGGSGYNKQSQMSAAHDGAQGYPHSVRLSLP
                     PLGAIFFIGPV"
     gene            complement(3769..6249)
                     /locus_tag="VGE92_04125"
     CDS             complement(3769..6249)
                     /locus_tag="VGE92_04125"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEY1313036.1"
                     /translation="MNTIDTQFANRRNVALQPGTLQAALLDRLTRTLGKNPASATQRD
                     IYDALSLAVREELTLRWLATQRRVANAHVKRVCYFSVEYLPGRSLLNALSSLEGGLLQ
                     EARVALHDLGHDLDDIAAQEVDPGLGNGGLGRLAACFLDSLATLQYPAVGYGIRYDYG
                     IFTQVIGPDGGQREVASSWLRLRNVWETPRGNVRYIVRFGGRVTSAENAQMGDLHRWV
                     DTTDIYAIGFDQLIPGNRVPTVNHLRLWSGRAVTPFHIDAFNAGNYAAAVQEQVEAKN
                     LSRVLYPDDSTPQGKELRLKQQYFFVSASLQDILATHLSEGRSLASMPDSIAIQLNDT
                     HPTVAIPELMRLLIDEHGLTWAESWQITSAVFSYTNHTLLPEALETWPVAMFERLLPR
                     HLQIIYVINRDFLQAVETRYPGDHERRRHLSIIDDAGDRRVRMAHLAVVGSHRVNGVA
                     QLHSDLMRKHVFCGFADMFADRFINVTNGIAVRRWLKQSNPGLSALLTDRLGSAWEND
                     LEELGRIAGDADNAEFRRQFRGVKRTNKRRLADEVMRRTGVEISVDSLFDVQVKRIHE
                     YKRQLLNLLYVVTRYRHIVDNPHADIVPRTVIFAGKAAPGYAMAKAIIKLINNVARTI
                     NTDAAARDKLRVAFLPDYDVSLAQKIMPAADLSQQISTAGMEASGTGNMKLSLNGALT
                     IGTLDGANIEIRDHVGAENVFIFGLTADEVAARRAAGYRPRHEVEANPDLKATLDIIA
                     SGFFSPGRPDDAAPVISRLLSDGEPFLVLADFAAYAKAQQQVDALYLEQDEWSRKAII
                     NCLNMGYFSSDRSIREYADRIWAVKPVI"
     gene            6316..7062
                     /locus_tag="VGE92_04130"
     CDS             6316..7062
                     /locus_tag="VGE92_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY1313037.1"
                     /translation="MTTSPLAIGIDVGGSSIKCALVDLNSGAFVGERFSTPTPAQGST
                     DSLLSALHSVVAKVPGDHAVGLAFPSVIIKGTVRTAANLNKAWIGKSLGELASERLRR
                     PVVAINDADAAGLAELRYGAARDVPGTVLLLTLGTGIGSALFVDGKLVPNTEFGHLQV
                     AGREAEHRASGRAKIEQNLDWDGWTEQLNLVMKEMHALLWPDLIVLCGGITEEPEKFM
                     HKLKCEARLVIGTLRSEAGIVGAALATTVV"
     gene            7059..7559
                     /locus_tag="VGE92_04135"
     CDS             7059..7559
                     /locus_tag="VGE92_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEY1313038.1"
                     /translation="MIRAALAAVLSIALAACASTAPNAKSTPPAATSPAALPRASPSP
                     RTLMVDTAISMRGQPYRFGGAAPGGFDCSGLVQYAASGAGLHVPRTAHEQLRIGEPIA
                     RGELQPGDLVFMHLAHKQLHVGIAIDADRFVHAPSKGGVIRVDSLDAPPYARGFLGAR
                     RIIATP"
     gene            7607..8212
                     /gene="gstA"
                     /locus_tag="VGE92_04140"
     CDS             7607..8212
                     /gene="gstA"
                     /locus_tag="VGE92_04140"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HEY1313039.1"
                     /translation="MKLYYFPGACALSPHIVLLEAGLPYTLEKIDNASKKTASGSDYL
                     TINSKGTVPALELDDGRVLTEGPAIVQYLADQKPESGLAPRAGTFERYQLMEILNYIT
                     SEVHKGFSPLFNPKISADWKASALAGLDKKFAWLSSFLGSKPYLLGDKFSVADAYLFT
                     VLNWTGHLKIDLGKWPVLAAFQARVAGRPKVQQAMREEGLL"
     gene            complement(8216..>8891)
                     /locus_tag="VGE92_04145"
     CDS             complement(8216..>8891)
                     /locus_tag="VGE92_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1313040.1"
                     /translation="GETIAFVGPSGAGKSTAFQLLLRFYDPDSGQVLMDERRLADLRP
                     EDIRAQIGLVPQETVLFGASARDNIRYGRPGASDAEIEAAAVAAAADEFLRKLPQGYD
                     TFLGERGTRLSGGQRQRIALARAILKDAPILLLDEATSALDAESERLVQEAFEKLMRG
                     RTTVIIAHRLATVLKADRIVVMDHGHKIAVGTHAELLLQSPLYARLASLQFAAVTSEA
                     VDAGVL"
BASE COUNT         1688 a         2837 c         2730 g         1636 t
ORIGIN      
        1 ctcctgagcg ctgtcgctgg cgttcaacaa taacaacagg cgtccctcga gattgctgcg
       61 tttgccgaac caaacaccta aggtacgcag gttcccatcg tgccattcac cttgcttcat
      121 ctcggcgccg ccgacgttta accaggtcac atccttgacg ctggcgcgcg aggtcgcacc
      181 cttgagaaag gtctcgcgcc gaaattcttc gtgcctgcgg cgcaactgcg ccagcaattg
      241 cacgaagcgc agcaaatccg cgtggctgct cttgaggtgc caatcgaccc aggagatctc
      301 gttgtcctgg cagtaggcgt tgttgttgcc gcgctgcgtg cgcgcgaact catcgcccgc
      361 ctgcagcatg ggcacgccct gcgacaagaa cagcgtggcg agcatattgc gcatctggcg
      421 tttgcgcagc gcattcaccg ccgggtcgtc cgtcgggcct tccgcgccgc aattccagct
      481 caaattctcg ttgtgcccgt ccgcgttgct ttcgagattg gcctcgttgt gccgctggtt
      541 gtaggagacc aggtcgtaaa gcgtgaagcc gtcgtgcgcg gttggaaagt tgatgccggc
      601 ggtcggcttg cggccatcat gccgaaaaat atcgctcgag ccggcgaagc gctcggccag
      661 atcgccgagc ttgccggcgt cgcgccgcca aaacgcgcgc accgcgtctc gataacggtc
      721 attccactcc gaccagcccg cgggaaatcg cccgagctga tagccacccc accccacatc
      781 ccatggctcg gcgatcagct tgacgtaggc gagcgacggc tcggcgcgca gcgatttgaa
      841 cagggccgag tgctggttga agccgttgcc ctcgcgcccc aggacggtcg cgagatcgaa
      901 gcggaagcca tccacgtgca tctcgtcgac ccagtatttg aggcagtcga tgatcaaggt
      961 gcgcacttga gggtgttcgc aattgaccgt gttgccgcaa ccggtgacgt tctcatagaa
     1021 gcgcaggtct tgcggcatga ggcggtaata caccgaattg tcgaggccgc gcagcgacag
     1081 cagcgggccg gtttcattgc cttcggccgt gtgattgaaa acgacgtcca gaatgacctc
     1141 gatcctcgcc gcatgcaaag ccttgaccat cgccttgaat tcggcgactg catcgcgcac
     1201 cgcatattcg ttcgccgccg agaaccacgc cacggagtta tagccccaat agttgctgag
     1261 gcgccggtcg cgcaggaatt gctcgctggc gaaggcctgg cacggcaaca gctccaccgc
     1321 cgtcacgccg atggacttga ggtgttcgat taccaccggc accgtcagtc ccaggtattt
     1381 gccgcgccac tctgcgggta ccgcgggatg cagctgcgtg aagcccttga catgcagttc
     1441 atagatgatg gtatcgcgcc aaggaacggc gggcggccga tccgcgcccc actcgaaact
     1501 gccgtcgacg acacgcgcac gcagcggcca gccgcaggac agtgcgcgcg catagggatc
     1561 gatcaacggc gccgtggcat cgaagcgatg acccttctcc ggctggttcg gtccatgcac
     1621 ataaaacgca taatgggttt ccgtgcccgc gcgccgcgaa gagagcaacc catgccaaat
     1681 gtcgccgctg cgtgccggca gatcgaagcg ggcaagttcc gcgccgctgg cggggtcgaa
     1741 taagccgacc tcgacccggg tcgcatggct cgaaaagact gcgaaattga cgccgcgctt
     1801 gttcctcgcg gctcccaacg gtcgcggcaa cccggcctga ataggcttca ctatacgggc
     1861 ccgatgaaga atatggcgcc gagcggcggc aagctcaagc gcacggaatg cggataaccc
     1921 tgcgcgccat cgtgcgccgc gctcatctgc gattgcttgt tgtatcccga gccgccgaag
     1981 cgcgcgtcgt cggaatcgaa tattttctga taggacccga ggtccgccac cccgacgccg
     2041 tagttatcgc gcggcaccgg cgtcgcgttg aacacacagg tgatcggtgc accgccatcg
     2101 gtaccgaccg cgcgacgcga gaacgcccag atgctctcgt cggcattgtg cagatcgacc
     2161 cagcggaacc cattgtgatc gcaatcgctg ccgtagaatt gcggagtcga gcggtacaga
     2221 agattcagct cgcgtacgca atcgcgcaag gccgcgtgtt gcggcaccgc gagctgcgcc
     2281 cattcgagtt gttcgtagtc gcgccactcc tgccactgac cgaattccga acccatgaac
     2341 agcagcttct tgccgggatg cgcgcacatg taggcgagga acaagcggaa attagcgcgt
     2401 ttctgccatt catcgccggg catcttgtcg agcagcgagc gtttgccgtg cactacttcg
     2461 tcgtgcgaga ccggcagaat gaaattctcc gaccaggcgt agacgaatga aaatgtgatc
     2521 aactggtggt tgtagcgccg ataaattggg ttgagcgcca catagcgcaa gctgtcgttc
     2581 atccagccca tgttccactt gaaattgaaa ccgaggccgc cgtattcggg cggctgggtc
     2641 acgccggcga acgaggtcga ttcctcggcg atcgtcagca cgcccggaaa cgcggcgtgc
     2701 accgcattgt tcatctggcg cagaaaatcg atcgcctcca gattttcgcg tccgccgtcg
     2761 cggttcggca gccattcgcc gggcttgcgg ctgtagtcca ggtacagcat cgacgccacg
     2821 gcgtcgacgc gcagcccgtc gacatgatac cgatcgaacc agaacagcgc attcgcgacc
     2881 aggaagttgc gcacctcatg ccggccgtaa ttgaaaatct tcgtccccca ttccatgtgc
     2941 tcgccgagcc gcgagtccgc atgttcgtac aacgcgctgc cgtcgaaata cgagagaccg
     3001 tgatcgtcgc ggggaaagtg cgccggcacc cagtcgagga tcacgccgat ccccgcctgg
     3061 tggcagcgat cgatgaagta catgagatcg tgcggtgatc cgtagcgggc ggtggcggcg
     3121 tagtagccga cgatttgata accccacgag gcgtcgagcg gatgctccgc gacgcccatc
     3181 aactcgatat gcgtaaagcc catgtcgacg acatatggaa tcaactgctc gaccgcttcc
     3241 ggccagctca tgaacggtgg gctgcgcgcg tgccacggac ggcgccatga tcctaaatgt
     3301 acttcgaagg cattgaacgg cgtgcgcagc gggtcgcggc gcgcgcgctc tgccagccat
     3361 tgcgagtcgc cccagacgta gccgtcgagg caggcaacca acgagcagtt ctgcggacgc
     3421 agctgcatgg cgaacccgta cggatccgac ttgacgaaca agccgccggc gcggtcgcgg
     3481 atctcgtact tgtatgcgct gccgagcccg atgccgggaa tcgccaattc ccacacgccg
     3541 gaggaggcgc ggctgcgcat cacgttcgcg ccgccgtccc agtggttgaa gtcgcccacc
     3601 acgctgaccc gctccgcgtt gggcgcccat accgcaaatc gagtgccgct cgtgccggcg
     3661 tcgctgtaag ggtgcgcacc gagcttgtca tagatgcgct cgtgcttgcc ctcgccgaac
     3721 aaatacaggt cgaagtcgct caagctcgag tcgctcaatc gtttcccctc agatcaccgg
     3781 tttcacagcc cagattcggt cggcgtactc gcgaatactg cggtcgctcg agaaataacc
     3841 catgttcagg cagttgatga tcgccttgcg gctccattcg tcctgctcga ggtacaacgc
     3901 atccacctgc tgttgcgcct tggcgtaggc ggcgaagtcc gcgagcacca agaaaggttc
     3961 gccgtcgctc agcagtcggc tgatgaccgg tgcggcatcg tcgggacggc ccggcgaaaa
     4021 gaaaccggaa gcgatgatgt cgagcgtcgc cttcaaatcg ggattggcct cgacctcgtg
     4081 ccgcggccgg tagccggcgg cgcgccgcgc agcgacctcg tccgccgtca gaccgaagat
     4141 gaatacattt tccgcgccta catggtcgcg aatctcgata ttggctccgt ccaatgtgcc
     4201 gatggtcaat gctccgttca gcgaaagttt catgttaccc gttcccgatg cctccatgcc
     4261 ggcggtggag atttgttgcg acagatccgc cgcgggcatg atcttctgcg ccaacgagac
     4321 atcgtaatcc ggtagaaacg ccacgcgcag tttgtcgcgc gcggcggcat cggtgttgat
     4381 ggtacgcgcg acgttattga tgagtttgat gatcgccttg gccatcgcgt agccgggcgc
     4441 cgccttgccg gcgaagatca ccgtccgcgg cacgatgtcg gcgtgcggat tgtcgacaat
     4501 atgccgatag cgggtcacga catacagcaa attcagcaat tgacgcttgt actcatggat
     4561 tcgcttcacc tgcacgtcga acagggaatc aacgctgatc tcgacgccgg ttcgccgcat
     4621 cacctcgtcg gccagccgcc gcttgttggt gcgtttcacc ccgcgaaact gccggcgaaa
     4681 ctccgcgttg tcggcatcgc ccgcaatccg tcccagctct tcgagatcat tttcccacgc
     4741 cgagcccaag cggtcggtca agagcgcgga caggcccgga ttggactgct tcagccagcg
     4801 gcgcaccgcg atgccgttgg ttacgttgat gaaccggtcc gcaaacatat ccgcgaaacc
     4861 gcagaacacg tgcttgcgca tcaaatccga gtgcagctgc gcgacgccgt tgacccgatg
     4921 gctgccaacc acggccaggt gcgccatgcg cacgcgccga tcgccggcat cgtcgatgat
     4981 cgacaggtgc cggcggcgtt cgtgatcgcc ggggtagcgc gtttcaacgg cttgcagaaa
     5041 atcgcggttg attacgtaaa tgatctgcaa gtgccgcggc agcagccgct cgaacatcgc
     5101 aaccggccag gtctcgagcg cctcgggcaa caaggtgtgg ttggtgtacg agaatacggc
     5161 gctggtgatt tgccagcttt ccgcccacgt caagccgtgc tcgtcgatca acaggcgcat
     5221 cagttcaggg atcgcaaccg tgggatgggt atcgttcaac tgaatcgcga tcgagtcggg
     5281 catggacgcg agcgaacggc cctcgctcaa atgcgttgcg aggatatcct gcaggctcgc
     5341 gctgacgaaa aagtactgct gcttgagccg caactccttg ccctgcggcg tcgaatcgtc
     5401 gggatacagc acgcgcgaca gattcttggc ttcgacctgt tcttgcacgg cggcggcgta
     5461 attgccggcg ttgaaggcat cgatgtgaaa cggggtcacg gcgcggccgg accagagccg
     5521 caagtggttg acggtcggga cgcgattgcc cgggatcaat tgatcgaacc cgatggcgta
     5581 tatgtccgtg gtatcgaccc agcgatgcag gtcgcccatc tgcgcatttt ccgcggaggt
     5641 gactcggccg ccgaaacgca cgatgtagcg tacgttgccg cgcggtgttt cccagacgtt
     5701 gcgcagccgc agccagctgc tggccacctc gcgctggccg ccgtcggggc cgatgacctg
     5761 ggtgaagata ccgtagtcgt agcggatgcc gtaaccgacg gccgggtatt gcagggtcgc
     5821 cagggagtcc aggaaacacg ccgccaagcg gcccaagccg ccgtttccca agcccggatc
     5881 gacctcctgc gcggcaatgt catcgaggtc atgacccaga tcgtgcaatg cgacccgggc
     5941 ttcttgcagc agccccccct ccaggctcga aagcgcgttc aacaaggagc ggcccggaag
     6001 atactcgacc gagaaataac acacccgctt gacgtgcgca ttcgccacgc ggcgctgcgt
     6061 ggcgagccag cgcaaggtga gttcttcgcg cacggcaagc gacaaggcat cgtaaatatc
     6121 ccgttgagtc gccgaagcgg ggttcttgcc gagagtgcgg gtcaggcggt ccagcagcgc
     6181 cgcctgcagg gtgccgggtt gtaaagccac attgcgacgg ttggcaaact gggtatcaat
     6241 cgtattcata ttaggcgtta ttataggggc gttgacaaag ggacggtgag cgaacctcac
     6301 aatgaggaat aaaagttgac tacttccccc ttggctatcg gcatcgacgt cggcggatcg
     6361 tccatcaaat gcgcgctggt ggacttgaat tcgggcgctt ttgtgggcga gcgattctca
     6421 acaccgacac ccgcgcaggg ctcgaccgac tccttgttga gcgccttgca cagcgtggtc
     6481 gcgaaagtgc cgggcgatca tgcggttggc ctcgcatttc cgagcgtgat aatcaagggc
     6541 acggtgcgta ccgcggcgaa tctcaacaag gcatggatcg gaaaatcctt gggcgagctc
     6601 gcgagcgagc gcctgcgccg cccggtggtc gccatcaacg acgccgatgc cgcgggtctc
     6661 gcggaactgc gttatggtgc cgcccgcgat gtgcccggca ccgtcctgtt gctgaccctg
     6721 ggaaccggca tcggcagcgc cctgttcgtc gacggcaagc tggtgccgaa cactgaattc
     6781 ggccaccttc aggtcgcggg ccgcgaggcg gaacaccgcg cttccggccg cgccaagatc
     6841 gagcagaacc tcgactggga cggatggacc gagcagttga atctggtcat gaaggaaatg
     6901 catgcactgc tgtggcccga tctcatcgtg ctctgcggcg ggatcaccga agagcccgaa
     6961 aaattcatgc acaaactcaa atgcgaggcc cgcttggtga taggaaccct gcgctcggaa
     7021 gccggcattg tcggcgccgc tctggcgacg actgtggtgt gatcagagcc gcgctcgccg
     7081 ccgttttgtc gatcgcgctc gctgcctgcg cgagtacggc gcccaacgcc aaatcgacgc
     7141 cgccggctgc aacctcgccc gctgcgctgc cgcgcgcgag cccaagcccg cgcacgctca
     7201 tggtcgacac cgcgatttcg atgcgcggtc agccgtatcg cttcggcggc gccgcgcccg
     7261 gcggcttcga ttgcagcggc ctggtgcaat atgcggcgag cggcgccggc cttcacgtgc
     7321 cgcgcaccgc gcacgaacag ctgcgcatcg gcgagccgat cgcgcgcggc gaattgcagc
     7381 ccggcgatct ggtgttcatg catctggcac acaagcaact gcacgtcggc atcgccatcg
     7441 acgccgatcg cttcgtgcac gcgccctcga agggcggcgt catccgcgtc gactcgctcg
     7501 acgcaccgcc gtacgcgcgc ggcttcctcg gcgcgcggcg cattatcgcc acaccttaaa
     7561 gcgcgtacaa tcgctgcaaa tcctccgcca atcaaaggac ctttctatga agctgtatta
     7621 ctttcccggt gcgtgcgcgc tctcgccgca tatcgtgctg ctcgaggccg gcctgccgta
     7681 cacgctcgag aaaatcgata acgccagcaa gaaaaccgcc tccgggtcgg attatctgac
     7741 gatcaattcc aagggcaccg tgcccgcgct cgagctcgac gacggccggg tgctgaccga
     7801 aggaccggcc atcgtccaat atctggccga tcagaaaccg gaatccgggc tcgcgccgcg
     7861 cgctgggaca ttcgagcgtt accagctgat ggaaatcctc aactacatca cctcggaggt
     7921 gcacaaggga ttctcgccgc tgttcaatcc gaagatttcc gccgattgga aagcctccgc
     7981 gctcgccggc ctcgacaaga aattcgcatg gctgtcgagc ttcctcggca gcaagccgta
     8041 cttgctgggc gacaagttca gtgtggcgga cgcgtacctg ttcaccgtct tgaactggac
     8101 cggccatctt aaaatcgatc tcggcaaatg gccggtgctg gctgcgtttc aggcgcgcgt
     8161 ggcagggcgc cccaaggtgc agcaagccat gcgcgaagag ggcttgctct agtcgtcaga
     8221 ggacgcctgc atcgaccgct tcgctcgtca ccgccgcgaa ttgcagcgat gccagccgcg
     8281 catacagcgg gctttgcagc agcagctcgg cgtgagtgcc gacggcgatt ttgtgcccat
     8341 ggtccatcac cacgattcgg tccgccttga gcacggtggc gagacgatgc gcgatgatga
     8401 ccgtggtgcg cccgcgcatg agtttttcga acgcttcctg gaccagccgt tctgattctg
     8461 cgtccaatgc gctggtcgct tcatcgagca acaagatggg cgcatccttc aagatggcgc
     8521 gcgccagggc gattcgctgc cgctggccgc ccgacaagcg ggtgccgcgc tcgccgagga
     8581 acgtgtcgta gccttgcggc agcttgcgca agaattcgtc cgccgctgcc gccaccgccg
     8641 cggcttcgat ctcggcatcg ctggcgcccg ggcggccgta gcgaatgttg tcgcgcgcgc
     8701 tggcaccgaa cagtacggtt tcctgcggca ccagaccgat ctgcgcgcga atatcctccg
     8761 ggcgcagatc cgcgagccgc cgctcatcca tcaacacctg cccggaatcg ggatcgtaga
     8821 agcgcagcag cagttgaaag gccgtgcttt tccccgcgcc ggatggcccg acgaacgcta
     8881 tcgtttcacc c
//
LOCUS       DASWZT010000152         2172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_49434, whole genome shotgun sequence.
ACCESSION   DASWZT010000152 DASWZT010000000
VERSION     DASWZT010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49434"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /locus_tag="VGE92_04150"
     CDS             complement(<1..1373)
                     /locus_tag="VGE92_04150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY1313041.1"
                     /translation="MGGGTKRRARAVVAMLQGLCLMLPLGGCLLTGDKPDPALDIPQA
                     YDRASKDPALAEAAVPSLDWWRGFRSKELTEIIEEARSANLDIAAAVAQIEQADAQSR
                     VAGAPLLPNVAANGSATRSRSSQTLGGGLGSAGGSSENNDLTASLTASYEIDFWGKNR
                     AALRAAEETAVASRYNREVVGLATVVSVANTYFQVLAAQDRVHIARENVASAAGILKL
                     IRQQMSAGTVSDLNVAQQESLLNTQRASIPPLVQTLQQNQVALALLIARPPEHVIVRG
                     GSMRGIAIPRVTPGLPSELLAQRPDIRQAEAQLASANANVANARAQFLPSITLTGEGG
                     YESAILKTLFRPESAFYTLTAGLTQPIFDGGRIQGNFDLQKGMQDQLLQNYRKAVISA
                     FTDVEKALIAVRETTLTESLQRDVVNSSRRAFKLSDQQLRAGTVNLVTLLQVEQTLFQ
                     AEDALAQA"
     gene            complement(1450..1929)
                     /locus_tag="VGE92_04155"
     CDS             complement(1450..1929)
                     /locus_tag="VGE92_04155"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEY1313042.1"
                     /translation="MTIAQYAPAISRFSSRWLLLGSLALNLFFVGVALAMAIRAPAPP
                     AWDRNVFVRVEHIANTLPPADADLLRGQINANRQLIDDAQRQYHSAQDQIHETLRHVP
                     FEAEAMRAAMAKTRAARQNFDQVIQGVFAFSAAQMSQAGRSALADWPPGRKTTSNSQ"
     gene            complement(1926..>2172)
                     /locus_tag="VGE92_04160"
     CDS             complement(1926..>2172)
                     /locus_tag="VGE92_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313043.1"
                     /translation="SPLPRQKQPLWRLPNVLLDWQFAPAWSRVAALACCAAIGFVIGI
                     AGVDRRFDGADARLTVASSADLGAIVFEPEALTGARP"
BASE COUNT          380 a          672 c          716 g          404 t
ORIGIN      
        1 gcctgtgcta gcgcatcctc ggcctggaac agtgtctgct caacttgcag cagcgtgacg
       61 agattgaccg tgcccgcgcg tagctgctga tcggaaagtt tgaatgcccg gcgcgaactg
      121 tttaccacat cgcgttgcag gctttcggtc aacgtcgttt cgcggactgc aatcagtgct
      181 ttttcgacat cggtgaaggc tgatatcacg gccttccggt agttctgcaa aagctgatcc
      241 tgcatgcctt tctgtagatc gaaattgccc tggattctcc cgccgtcgaa gatcggctgg
      301 gtcaggccgg cggtgagcgt ataaaatgcc gattccggcc ggaacagcgt cttcaagatc
      361 gcgctctcgt aaccgccttc gcccgtgagc gtgatgctcg gcaggaactg cgcgcgcgca
      421 ttggcaacat tggcattggc ggaagcaagt tgtgcctccg cttggcggat atcggggcgc
      481 tgcgcgagca gttccgaggg caatccgggt gtaacgcgcg gaatcgcgat gccacgcatg
      541 ctgccgccgc ggacaatgac gtgctcaggc ggtcgggcta ttagcaacgc caacgctact
      601 tgattttgtt gaagcgtttg caccagcggc gggatcgacg cccgctgcgt gttgagcagg
      661 ctctcctgct gcgcgacgtt gagatcggac acagtgccgg cggacatttg ctgccggatc
      721 agcttgagga tgccggccgc actcgccacg ttttcgcgcg cgatgtgcac tcgatcctgc
      781 gccgccagca cctgaaaata cgtgttagcg acactcacga cggtcgctaa accgaccacc
      841 tcgcggttat agcggctggc gaccgcggtc tcctcggcgg cgcgcagagc cgcccggttc
      901 ttgccccaga agtcgatctc gtagctggcg gtgagcgagg cggtgaggtc gttgttctct
      961 gaacttccgc cggcgctgcc taagccgccg ccaagcgtct gcgaggaacg cgaacgcgtg
     1021 gcgctgccat tggccgcgac attcggcaat agcggcgcac cggcgacgcg cgactgcgcg
     1081 tcggcctgtt cgatttgagc gaccgccgca gcgatatcga gattggccga gcgcgcctct
     1141 tcgatgatct cggtcagttc cttggagcga aagccccgcc accagtcgag cgagggcacc
     1201 gctgcttccg ccagcgcggg gtctttcgac gcgcgatcat aagcctgcgg aatatcgagc
     1261 gcgggatcgg gcttgtcgcc ggtgagcagg cagccgccga gcggcagcat caggcaaagt
     1321 ccttgcagca tggccacaac cgcgcgcgcg cgccgttttg ttccgccccc cacaccctgg
     1381 ctaccgcgac gcataaggac gactttcaag aactactgaa acacgggtcg ggcgggaaat
     1441 tagcctgttt tattgcgaat tgcttgtcgt tttacgcccc ggcggccaat cggccaaagc
     1501 gctgcggccg gcctgcgaca tctgcgcggc cgagaacgcg aacacgcctt ggatgacctg
     1561 gtcgaagttt tgccgcgcgg cgcgggtttt cgccatggcg gcgcgcattg cctcggcctc
     1621 gaacgggaca tgccgcagcg tttcgtggat ttgatcctgc gcggagtggt attgccgttg
     1681 cgcgtcgtcg atcagctggc gattggcatt gatctggccg cgcaacaggt cggcatcggc
     1741 cggcggcaga gtgttggcga tgtgctcgac gcgcacgaac acgttgcggt cccaggccgg
     1801 cggggccggc gcgcggatcg ccatcgccag cgcgacgcct acgaaaaaca agttgagcgc
     1861 cagcgagcca agcagcagcc agcgcgacga gaagcgcgaa atcgcgggcg catattgagc
     1921 gatggtcatg ggcgtgctcc ggtcagtgct tcgggttcga acacgatcgc gccaagatcg
     1981 gcgctgctgg cgaccgttaa tcgggcgtcg gcgccgtcga agcggcgatc gaccccggca
     2041 ataccgatga cgaagccgat ggccgcgcaa caggccaagg cggcgacgcg cgaccaggcc
     2101 ggcgcaaatt gccagtcgag cagcacgttt gggaggcgcc acagcggctg cttttgccgc
     2161 ggtagcgggc tc
//
LOCUS       DASWZT010000153         3705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_93096, whole genome shotgun sequence.
ACCESSION   DASWZT010000153 DASWZT010000000
VERSION     DASWZT010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93096"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VGE92_04165"
     CDS             <1..676
                     /locus_tag="VGE92_04165"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEY1313044.1"
                     /translation="FMCGLPFSRFEPRPELLAAPVPSPAEFRGLPQYWSELVVRKDSL
                     FERVQDTFGGRIALTVPGSQSGCLAALYYLMTVADRSPLYREVIAPQITPRGAMAAVI
                     DGAADVAPIDSYAFCLLQKYQGALTSQLRIIGRTTPTPIPPLVASEPGAGALQAALLD
                     AHRIVELKPLLSGLLLERFARPDPASYDALRLNFEAAGRYWREHRLAAQLHPEFAASE
                     VLRPAG"
     gene            698..1600
                     /locus_tag="VGE92_04170"
     CDS             698..1600
                     /locus_tag="VGE92_04170"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY1313045.1"
                     /translation="MQFLKRTFFQFDGPTWVVALVLYGAWVALVWFNAVLPWWVIMPV
                     GAYLVAWHFSLQHEAIHAFRGTPAWLRFAVVFPPLGLWFPYPLYRKSHSTHHRDVNLT
                     IPGVDTESYYVLQADWARMGAFKRKLLLFNQTLAGRLLIGPLLRLSLLVVRETRRVRQ
                     GDFSHLPHWAAHVIAVALLFGFISGFCHFPWWQYCLLVAYPGLSLGLLRAFTEHRAAD
                     DSQQRTAAVESNVLFGLLYLYNNLHVAHHLKPTMPWYEIPRFYRQHRQELLASNGEFV
                     YSGYALLARRFFLTPVFSPVHPTL"
     gene            1610..2911
                     /locus_tag="VGE92_04175"
     CDS             1610..2911
                     /locus_tag="VGE92_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1313046.1"
                     /translation="MPSKTSAIVATAIGNMLEWYDFTVYALFAGYIAGNFFPGGDSNT
                     RLLKTFLVFGLGFVVRPLGALLIGNFADRAGRKAALTLTILLMAVGTGIIAFAPTFAA
                     AGFAAPVLLLLGRVLQGFSAGGEIGGAAAYLLESADAAERGRAASWLEASMGISNILG
                     ALVAFGVTALLSAAQVQAWGWRIPFIIGLAIAPVGLYLRRTLHETDAFQEESRRRARD
                     PAQRKAPLLEIFRLHGRSLFVGLCVATLWAVAVYVLMIYLPTYVQHPETFNFSARQAF
                     GASLIGNIPFVIACVWFGSLSDRIGRRRSLAISATLLLVLVLPLFLWLKSSPTLATLI
                     VVESIVCILVASFVGVAPAALSEIFPTGVRSTGISIVYNGAFTLFGGFAPMILTWLTQ
                     RSGGSIYSPAWYVMLAAAVGLIAIPFVGARESALEPALAMQ"
     gene            2962..3339
                     /locus_tag="VGE92_04180"
     CDS             2962..3339
                     /locus_tag="VGE92_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY1313047.1"
                     /translation="MKTLIAAFSLILCTTLAMSAEVPFNQAQFDAARAAGKPVAVVFH
                     ADWCPTCRAQAPVLKQLAQSPQLQSLTVYVANFDTDKALKKSLGVTQQSTIVVFKDGK
                     ESARSTGDTQKDALAALLRHAVS"
     gene            3352..>3705
                     /locus_tag="VGE92_04185"
     CDS             3352..>3705
                     /locus_tag="VGE92_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313048.1"
                     /translation="MDFGPATYGLGLIAGALSTLSPCVLPLLPVLIASAVSAHRWGVI
                     ALAIGLALSFMIVGIILATLGTSLGLDPDTFRTIGALVLALFGLVLLVPKLQDLFAHA
                     TRLFSNSGASVLARMT"
BASE COUNT          549 a         1152 c         1129 g          875 t
ORIGIN      
        1 cttcatgtgt ggtctgccgt tctcgcgctt tgagcctcgc cccgagttgc tggctgcacc
       61 ggtgccctcg cccgcagaat ttcgcggcct gcctcaatac tggagcgagc tcgtggtgcg
      121 caaggacagc ttgttcgagc gtgtgcagga tacatttggc ggccgcatcg cgctgacggt
      181 gcccggctcg cagtccggct gcctggcggc gctctactac ctcatgaccg ttgcggacag
      241 atccccgttg tatcgcgagg tcattgcgcc ccaaatcacg ccgcgcggcg cgatggccgc
      301 ggtgatcgac ggcgcggccg acgtcgcacc cattgattcc tacgccttct gtctgttgca
      361 aaagtaccag ggcgcgctca catcgcagct gcggatcatc gggcggacca cccccacgcc
      421 catcccgccg ttggtcgctt ccgagccggg cgccggcgcc ctgcaggccg cgttgctgga
      481 cgcgcaccgc atcgtggaat tgaagccgct gttatcgggt ctgttgctgg agcgcttcgc
      541 gcgcccggat ccagcctctt atgacgcgtt gcggttgaat tttgaagcgg ccggccgata
      601 ttggagggaa caccgcctgg ccgcgcagct gcaccccgaa tttgcggcca gcgaggtttt
      661 gcgacctgcg ggttagccgt taaactgccg gcgggccatg caatttctga agcgcacatt
      721 ctttcaattc gatggaccta cctgggtggt ggccttggtg ctgtacggcg cctgggtggc
      781 gttggtttgg ttcaacgccg tcctgccatg gtgggtcatc atgccggtcg gcgcctatct
      841 ggtcgcctgg catttttcgc tgcagcacga ggccatccat gcgtttcgcg ggacccctgc
      901 gtggctgcga tttgcggtgg tgtttccgcc gttggggctg tggtttccct acccgctgta
      961 tcgaaagagt catagcaccc atcatcgcga cgtcaatttg acgattccgg gcgtcgacac
     1021 cgaatcctac tatgtgctgc aagcggattg ggcgcgcatg ggggccttca agcgcaagct
     1081 gttgctgttc aaccaaaccc tggccggacg cttgttgatc gggccgctgc tgcgcttgtc
     1141 gcttctggtc gtcagggaaa cacggcgcgt gcggcagggc gatttcagcc acttgccgca
     1201 ttgggccgcg cacgtgatcg ccgtcgccct gctcttcggg ttcatttccg gtttctgcca
     1261 ttttccatgg tggcagtact gtctgctggt ggcctacccg ggcttgagct taggattgct
     1321 gcgtgccttt accgaacatc gcgccgccga tgactcgcaa cagcggaccg ctgcggtcga
     1381 gtcgaatgtg ctgtttggcc tcctgtacct atacaataat ctgcacgtcg cgcatcattt
     1441 gaaacccacc atgccgtggt atgaaattcc gcgcttctac cggcaacatc ggcaggagct
     1501 gttggcgagc aacggagaat ttgtttactc aggatatgcg ttgttggcgc ggcgcttctt
     1561 tctgaccccc gtattcagtc ccgtccatcc cactttgtag aggattgcat tgccgagcaa
     1621 aaccagcgcg atcgtcgcga ccgcaatcgg caatatgctc gaatggtacg acttcacggt
     1681 gtacgccttg tttgcgggct acattgccgg caacttcttc cccggtggtg actcgaatac
     1741 ccgcctgctg aagacattcc tggtcttcgg cctcggcttc gtggtacgtc ccttgggggc
     1801 cttgctgata ggcaacttcg ccgatcgcgc gggtcgtaaa gcggcgctga cactgaccat
     1861 tctgttgatg gcggtgggca ccggcatcat tgcgtttgct ccaactttcg ccgccgccgg
     1921 ctttgctgcc cccgtgcttc tgttgttggg gcgcgtgctg cagggtttct cggctggcgg
     1981 cgagatcggc ggcgccgccg cctacttgct cgagagcgcc gatgcggcag agcgcggccg
     2041 cgccgcatca tggctcgagg ccagcatggg gatctcgaat atcttgggcg cgctggtggc
     2101 gttcggggtc accgcccttc tcagtgccgc gcaggtgcaa gcctggggat ggcggattcc
     2161 cttcatcatt ggactcgcca tcgctccggt gggtttgtat ttgcgccgca cgttgcacga
     2221 aaccgacgcg tttcaagagg agagccggcg gcgtgcgcgg gaccccgcgc agcgcaaagc
     2281 ccctctgctg gagatatttc gcctgcacgg acgcagcctg ttcgtcggct tgtgcgtcgc
     2341 cacactctgg gcggtcgccg tgtacgtgtt gatgatttat ttgccgacgt acgtacagca
     2401 tccggagact ttcaatttca gcgcccgcca ggcgttcggt gcttcgctca tcggcaatat
     2461 tcccttcgtc atcgcgtgcg tgtggttcgg ttcgctctcg gatcgaatcg gccggcgccg
     2521 cagcttggcc atcagtgcga ccctgttgct ggtcctggtg ctgccgttgt ttttgtggct
     2581 gaaatcctcg cccactctgg ccaccttgat cgtggtcgag tcgatcgtgt gcattctggt
     2641 cgcgagtttc gtgggagtcg ccccggcggc cttgtcggaa atctttccga ccggggtgcg
     2701 ctcgaccggc atctccatcg tctacaacgg cgcgttcacg ctgttcggcg gatttgcgcc
     2761 catgattctc acctggctca cgcagcgctc gggcggctcg atttattcgc ctgcctggta
     2821 tgtgatgttg gcggccgcgg tcggattgat tgcgattccc tttgtagggg ctcgcgagag
     2881 cgccctcgag cccgcgctgg cgatgcaata aagcagctcg atccacggtc aactggtcat
     2941 gttcgtacat cggagcaccg catgaaaacc ctgatcgccg ctttttccct tatcctctgt
     3001 acaaccctcg ccatgtcggc cgaggttccg ttcaaccagg cacagttcga tgccgcgcga
     3061 gctgcgggca agcccgtggc ggtggtgttc catgcggatt ggtgtccaac gtgccgtgcg
     3121 caggcgccgg tactcaagca gcttgcacaa tcgccgcagt tgcaatcgtt aaccgtgtac
     3181 gtcgccaact tcgataccga taaggcgttg aagaaatccc tgggagttac gcagcaaagc
     3241 accatcgtcg tattcaagga cggcaaggaa agcgctcgtt ccaccggcga cacccagaaa
     3301 gatgccctcg ccgcgctgct gcgtcacgcc gtctcgtaac gagctgcggc gatggacttc
     3361 gggcctgcca cctacgggct ggggctgatt gccggcgcat tatcgacact gtcgccctgc
     3421 gtcttgccgc tgcttccggt cttgatcgcc tccgccgtga gcgcccatcg ttggggggtg
     3481 atagcgctcg cgatcggact cgcgctgtcg tttatgatcg tcggcatcat tcttgcgaca
     3541 ttgggcacat cgctcggcct cgatccagat acctttcgca ccatcggcgc gctcgtcctc
     3601 gcactgttcg gtttggtttt gctggtgccc aaactgcagg atttatttgc gcacgctacc
     3661 cgcttgttca gtaattccgg cgcttctgtg ctcgcgcgta tgact
//
LOCUS       DASWZT010000154        15126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_68843, whole genome shotgun sequence.
ACCESSION   DASWZT010000154 DASWZT010000000
VERSION     DASWZT010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15126
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68843"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VGE92_04190"
     CDS             complement(<1..547)
                     /locus_tag="VGE92_04190"
                     /inference="COORDINATES: protein motif:HMM:NF039473.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HEY1313049.1"
                     /translation="MSSDISEPLTSLNYKDVRTVARKALAQLLEPLVGFVLDSGLSTQ
                     ELRWILREAAVRSVAAKQLSAMRRLNISGIAASTGIPRADISRILRSSARPSPSNTDR
                     QQQSTNRILAVWHEDPRFTTIEGKPADLKIYGRGATFDALVRGHGRGIPTRAMLDELT
                     RVGAAEVLASRLVRARTSVAID"
     gene            768..1889
                     /locus_tag="VGE92_04195"
     CDS             768..1889
                     /locus_tag="VGE92_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313050.1"
                     /translation="MGDSPAKLLAHLIRPARGGSAGVIIVFSFFFSLLARGGLLAIPG
                     VVILTSWFFKYAYILFDHTVYGFDEPPILDINMLNPFSELRPLAQLAIIGIVYVAIHF
                     AQTSLGTPAAIALSVLAALLLPASVAILGVDRHLLKAVYPVAWVRLILALGPLYLLVL
                     AIIGGYALLILLLQRWELWFPLQIAIYLFCILSAFSLLAGVMYERRHELGLDVWVSPE
                     RTEDVLRKQALHRSELEVTAAYGLSRAGSHSRAWEHLQALLSARGHAPEGYRWLCEHV
                     VSWDDPRYITRLTEDHVERLLTLKRTGEALETVARRLQADPSFRPKTGAATLQIARIA
                     SEGGGKPWVARALLADFPSRYAGDPNVAAATALREKLTR"
     gene            2055..3446
                     /locus_tag="VGE92_04200"
     CDS             2055..3446
                     /locus_tag="VGE92_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEY1313051.1"
                     /translation="MLLSDGAARAHYRRYAEWLTEQPPERLAQKRAEADALFHRVGIT
                     FAVYGQEEGTERLIPFDIVPRVLPLEEWIRLENGLKQRVRALNAFIHDIYHDQQILRA
                     NVIPAEQVLCNSQYRPEMQGVDVPGGIYAHIAGIDIVRDDAGEFRVLEDNLRVPSGVS
                     YMLENRKMMMRLFPELFSGHRVAPVDHYPDVLLDNLRSVAPTGVADPTVVLLTAGAHN
                     SAYFEHAFLAQQMGIELVEGQDLFVRDEAVFMRTTQGPQRVDVIYRRIDDDFMDPLVF
                     RPDSMLGVPGLLTAYRAGHVNIANAIGTGVADDKSIYPYVPDMIRFYLGQSPIIGNVH
                     TWMLRRKEDKDYVLAHLPELVVKEVHGAGGYGMLIGPAATQAEIDLFRRQIIATPERY
                     IAQPTLALSTCPTFADRNLAPRHIDLRPFVLSGRDVTIVPGGLTRVALREGSLVVNSS
                     QGGGTKDTWVLET"
     gene            3446..4393
                     /locus_tag="VGE92_04205"
     CDS             3446..4393
                     /locus_tag="VGE92_04205"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEY1313052.1"
                     /translation="MLSRTADHLYWMSRYIERAESLARLVDAHYRMSLLPHSGENLAQ
                     SLSSTMTALHMDEPYRKKFDTVEPRTVFEFLSLDRDYSGSIVSCLRAARENARAVRGS
                     LTSELWETLNSTWLEARSLVANLSPSPDIGHFVEWVKERSHLTRGVTIGTMMRDEAFH
                     FTRIGTFLERADSTARILTAHQGDLVPGADASAVPDPYQWSVLLRALSAFEVYRRAFR
                     DVITPLRVAQLLILRDDMPRSLLRCCKEVYANLKAVSNDQSAETERRAGEMHAMLHFT
                     RMEEITAGGLPRFLEQFLARVRDLGDRIASDFLLPPAEV"
     gene            4399..5202
                     /locus_tag="VGE92_04210"
     CDS             4399..5202
                     /locus_tag="VGE92_04210"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY1313053.1"
                     /translation="MQMHIRHETRYRYERPVKYSVQSLHLTPRRDLSQRALTWSITSP
                     GRRLEQVDAYGNISHLLTIEEPHREIQIVVRGVVETVDTEGRQDDGPLSPLAYLAPTQ
                     LTAPNDDLRAFAQQALHTVTEPRARAQALADAVFEAVRYKSGTSDVQDSAAVAFRSGE
                     GVCQDHAHVYLASARAVGIPARYVSGYLYTGDASDAASHAWVDVWLGTEIGWQSMDVT
                     HKRPAVRTYCRLAVGRDYLDAAPVRGVRQGGGGEKMEANVLVAESAQQQ"
     gene            5219..5947
                     /locus_tag="VGE92_04215"
     CDS             5219..5947
                     /locus_tag="VGE92_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-type protease"
                     /protein_id="HEY1313054.1"
                     /translation="MTYCVGLLVDTGLVMLSDSRTNAGVDQINTFRKMATFQRANDRV
                     LVLMSAGNLAITQAVVNLLHEPPAEADQPTRIYRASNMFNAARVVGDALREIHERDGE
                     SMSEHGHDFNGTFILGGQIKGEEPRLFHIYSAGNFIESSPDTSYFQIGESKYGKPIID
                     RVISRSSSLAQAAKCALVSMDSTIRSNLSVGPPLDLAVIRRDEYRLATHISIDMDNEY
                     FTLIRKRWGFALKEVFSELPNPNW"
     gene            complement(5974..6348)
                     /locus_tag="VGE92_04220"
     CDS             complement(5974..6348)
                     /locus_tag="VGE92_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313055.1"
                     /translation="MNTQPLVTATLIGTILQIGMVLAGHWIPAVQGAFMFGGLGLSCI
                     AGAIYELRARGSWGGAAVGGVIAGGVSALAGIAVSWLLGDVPATLLLLGTSGSAVTGL
                     LGGVAARLLRPKAATGGVAGNL"
     gene            complement(6362..6973)
                     /locus_tag="VGE92_04225"
     CDS             complement(6362..6973)
                     /locus_tag="VGE92_04225"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY1313056.1"
                     /translation="MNLRSGTLLCLGVGALLGRAPAADFPDVRDASYTEPNGAHVLQL
                     SIRIRAPVSKIWNLFISAEGWRSWAVPVAWVDFGVGGLVETSYNATAVRGAPGNIKNS
                     IVAYVPERLLVLRNIQAPPGFEHAEDFAKTVTVINLRSVGKDSTEVEMDGVGFLATPA
                     FDALLQKFRFGDSWTLEHLKRAAEHGPVDWVAEEQSDKPRQSK"
     gene            complement(6970..7578)
                     /locus_tag="VGE92_04230"
     CDS             complement(6970..7578)
                     /locus_tag="VGE92_04230"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY1313057.1"
                     /translation="MEDESTRERLITSALRLFAEKGYQSTSVADLLQESKCHSGSFYH
                     FFPTKQDLLLAVLERYRLGIVPMLIEPAWKNVHDPIERIFALLGRYRMLLRTTDCAYG
                     CPIGSLALEIHEPDPAVRKLLADNFTGWVDHVERCLIEARATLGPDVDTRRVASFVLT
                     VMEGGVMQSRTYRNLKAFDAAVASLRDYMNSLRRVASRQATS"
     gene            7758..8096
                     /locus_tag="VGE92_04235"
     CDS             7758..8096
                     /locus_tag="VGE92_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313058.1"
                     /translation="MTDEYDDRVPSPLLRCLGIVGAAFLFLYGVVGVLHNDLKVAHAK
                     SGPGVHLHGLLAWVCFAGMVMMSIGGIRFLAPGDGKFDFSGRRRRFGPLIVVGLVLYL
                     AAQAIAGLRS"
     gene            8225..8533
                     /locus_tag="VGE92_04240"
     CDS             8225..8533
                     /locus_tag="VGE92_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313059.1"
                     /translation="MNIKIFIVTLLASFAAQAGDWTDMLADLMQQPDTRIEFIQSMRD
                     LIKADGYVCYAPRHIRSVDSALYVVECDHEADGATSLYSVSATPMGNGLVWHATPRQS
                     "
     gene            complement(8544..9146)
                     /locus_tag="VGE92_04245"
     CDS             complement(8544..9146)
                     /locus_tag="VGE92_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313060.1"
                     /translation="MDYSTRWGLPRYLTLVGVLLIHGALLAALFMVSRTEQIARLMDD
                     PVEVQFFPPNTMPRIRADSSRKRGFNGDHAISVTAPVLASAAPPLSASGADAGSGAGV
                     DWKAEARRAVQAYEIRTNQPPDSLLTGSPAEENWWPRTRHHAGEQFKTPSGDWIVWVN
                     SDCYQVASASGHSYAPGPRLTETICVHDEKPRQSGSAAAN"
     gene            complement(9183..10412)
                     /locus_tag="VGE92_04250"
     CDS             complement(9183..10412)
                     /locus_tag="VGE92_04250"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1313061.1"
                     /translation="MVFLLAAVLFINYVDRGTMPTAAHLMQADLHLNAAEMGVLFGAF
                     YWTYTCMQIPVGWLAERFGPHRILAAGLTIWASATMLTGIAHTFASLLVLRLLLGLGE
                     SSGFPSVAKILAAVVPPKGLGTANGIVALAYLVGPAVGTYAGGMLMANFGWRSSFLVF
                     GALSLLWLLPWSRVAGEVRTALKPKPNEDSPTWGMILRQPALWGTGLGLFSSNYLFYF
                     MLNWLPSYLQTERGMSLVRMTQLATLAYTVGAAAAFLGGWAIDRYIARGGSPNVGYKS
                     VMAIAHIGYVPCMWFMAAGSPTLMLAGMFIYQILCGASSPGVYAMCQILAGPRASGRW
                     VGVENSIGSLAGVAAPTITGFIIQITGHYTYAFVAAGAMSLFGLIGWIWLVPTLQELS
                     WKSAGKAGAAVEEPSPV"
     gene            complement(10463..11236)
                     /locus_tag="VGE92_04255"
     CDS             complement(10463..11236)
                     /locus_tag="VGE92_04255"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF005451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEY1313062.1"
                     /translation="MNASLKPVEPAADVERRARVDLAACYRLADRFGLNEGIDNHLTL
                     LVPGYADRFFLAPFGMHWSEIRASDFLVVGFDGEVLSGKGAVEDTALYIHQPVHRLSP
                     QGRCVLHTHMPYATALCMLQNPRLEMAVQSALGFYNDVAYDPTYNGLAFDLTEGERLA
                     AALGDKSVLMMANHGVLVVGKTIPLAFERLYFLERAAQAQVLALSTGRPLRLIPDALI
                     EKTVAQFGAGATVGGRDRADLHFDALKRVLDRAHSDYAS"
     gene            complement(11266..13005)
                     /locus_tag="VGE92_04260"
     CDS             complement(11266..13005)
                     /locus_tag="VGE92_04260"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY1313063.1"
                     /translation="MNKIKSAGMMSGLGGLLCLAGMCAQAWAAAANVKPASGDNPFDQ
                     LVDDFVMGTLALSPTGATGFGYHVHHGVSLDDQLDDFSAAGIEAQRSFLHDIEIRIGH
                     FDGAALDPEQRADVDIMRNALGATRLELDEIQSYRHNPTTYVELLGNGLYSPYVLHYA
                     PAPERYRHIISRLQKVPELIQQAETNLQDSPEVWNRVAREENDGNVGLIDTTLRADCP
                     PDARMRYDQAAAAAMASLQSFNHWLENNLSERKSDWRLGKERYAKKFRYTLATGKTPE
                     ALLAEAEADLVKVRAEIARLAAPKTVEEALADVARQHATAASFMASAKQALAGATAFV
                     KAKDLLTLPPNANLEVIDTPVFMRGIYSVGGFNPAPALEPKLGAFFWVTPIPSDWPQT
                     RVDSKLREYNASGMQHLTVHEAMPGHYVQAEYANDVQPRSRRLLRNIFGNGPYIEGWA
                     VYAQQLMAEQGYLGDTPGYRLTLQKQLLRVLANTILDVRLQTLGMTDQQALDLMTKST
                     YQEMEEASGKLQRAKLASCQLPTYYAGYKGWLADREHYQAKHGAAFSLKQFHEAALRE
                     GEVPLPVLDQLLH"
     gene            13095..14522
                     /locus_tag="VGE92_04265"
     CDS             13095..14522
                     /locus_tag="VGE92_04265"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY1313064.1"
                     /translation="MKLRKIIRGTVIVLALLLLVLIAALWLKPPELLRIGANYAAKIV
                     CSNVFLAGRDPEEVLRTDVQAPGAALLHLMRVSVDRQQQVVRTGLLGFIGDGVALARP
                     DTGCAVVADGNVAYARQIGALAAAGLPTPDAASTDSPPGPNSGTAATTAALWPDGDAV
                     TPNPALDRVLADDTLAGPGVRAIVVVDHGRIVAERYGAGFSAKTPLLGWSMTKTVLAG
                     VVGALIKDGRLTLDQAAFWPKAAGDARDRIRIADLLAMSSGLRFNEGYGAVSDVTRML
                     YLQPDMPGFAHAQPLEHPPGGYWSYSSGSANILSRIAQDAAGPVGANYPREKLFKPLG
                     MTSATMETDEDGTLVGSSYMYATARDWARYGLFLMQAGVWHGQAMLPAGYVALMASPV
                     SASGGQYGHGLVWLWGSDGKGPGMNPDSAFGIPADTFWMMGHDGQSTAIIASRGLVVV
                     RLGLTPWQDRYQPQPLIKAVLAALP"
     gene            complement(14494..>15126)
                     /locus_tag="VGE92_04270"
     CDS             complement(14494..>15126)
                     /locus_tag="VGE92_04270"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY1313065.1"
                     /translation="VLVTVLYVIINIAILRVLPLSVLAASKLPAADVARVVLPRGGAE
                     LVTLISLVTVLSLINATILMAPRILFAIGRDGLFTEKAAAVSAGGTPRVALTISSVGA
                     AALIMSGTFEQIVAVAAVLFLLLYVSAYSALFVLRRREPETPRPYRAFGYPYTTAIVL
                     LGCVSLWIVAIVEDPRSALIAGIMLMASVPVYLYLSRRRSAATAALRVRP"
BASE COUNT         2853 a         4936 c         4545 g         2792 t
ORIGIN      
        1 gatctatcgc gacggacgtc cttgcacgca ctagccgaga ggcaagtact tctgccgcac
       61 caacccgcgt caactcatct aacatcgctc tcgtcggaat gcctcggcca tgacctctca
      121 ccagagcatc aaaagtggca ccacgtccgt atattttcag atccgccggc ttgccttcaa
      181 ttgtcgtgaa cctgggatct tcgtgccaca ccgcaagtat tcgatttgtg gattgctgct
      241 gccgatcggt atttgatggt gaggggcgcg cgcttgacct aagtattctc gagatatcgg
      301 cgcgcggaat acccgtggac gctgcaattc cggagatatt caaccggcgc atcgcactta
      361 gctgcttcgc tgcaacgctg cgaacggcag cttcccgcaa aatccagcgc agttcttggg
      421 tgctcagtcc ggaatcgaga acaaaaccga ctaggggttc caagagctgc gcgagcgctt
      481 ttctagcgac tgtccgtacg tccttgtaat tcaaacttgt tagtggttcg gagatgtcgg
      541 acgacacttt taaatgatcc agaatttgcc gacgtcacgt cgcctacgta cgtggtttga
      601 gtttcgcaac cacccgccga tcaacggccc aaaataatgg ctgtccccga ttgaggtcgg
      661 ccgaatttca aatgaatgca gagatatatt aagacagatt agtggaatga ccgagcggag
      721 ccacacattt cgcaagaatt tgcgcgcatc ggagctaaaa taggctcatg ggcgattctc
      781 cggccaaact cctcgcccac ctaatccgcc cggctcgagg cgggtccgcc ggagtcatca
      841 tcgtcttctc attttttttc tctctcctcg cccgcggcgg actcttggcg attccggggg
      901 tcgtgattct gacgtcttgg ttcttcaaat acgcgtacat cctcttcgac cacaccgttt
      961 atggtttcga cgagccgccg attctcgaca tcaacatgct gaatccgttc agcgagctgc
     1021 gtcctctcgc acaactggcg atcattggca tcgtctatgt cgccattcat tttgcgcaaa
     1081 cttcgctcgg cacgccggct gccatcgcct tgagcgtgct cgcggcgctc ttgctcccgg
     1141 cgtcggttgc gattctcgga gtcgatcgac atttgctcaa ggccgtctat cccgtggcct
     1201 gggtgcggtt gatactcgct ctgggtccgc tctacctttt ggtgctggcg atcatcggcg
     1261 gctacgcctt acttatctta ttgctgcagc gttgggaact atggtttccg ctgcaaatcg
     1321 ccatttatct gttttgcatc ttgtcggcat tcagtttgct ggccggcgtc atgtatgaac
     1381 gccgccacga actcgggctc gatgtgtggg tctctccgga gcgcaccgaa gacgtgctgc
     1441 gcaagcaggc cctgcatcgc agcgagcttg aggttaccgc ggcctatggg ctgagtcgcg
     1501 cgggctcaca tagtcgcgca tgggaacatt tacaggccct gttgtcggcc cgcggccatg
     1561 cacccgaagg ctaccgctgg ctctgcgagc acgtcgtctc ctgggacgat ccgcgctaca
     1621 tcactcgcct aacggaagac catgtcgagc gcttgctcac actgaagcgc accggcgagg
     1681 cactcgaaac cgtggcacgc cggctccaag ccgatccctc ctttcgtccc aagaccggcg
     1741 ccgctacgct gcaaatcgcc cggatcgcca gcgaaggcgg cggcaagccg tgggtcgccc
     1801 gcgccctcct tgcggatttt ccctcgcgct atgcgggcga ccccaacgtc gcggcagcga
     1861 ccgcgcttcg cgaaaagctc acgcgttgac gcaccaaagc cgcgcttcag ccaacccgtc
     1921 cgcgccttga tttggtgcag cattgcggcg caatagaggt aagtacatga ttgcgggtgc
     1981 cgcgagccgg tggcagggaa tttgctccaa tttcaatctg agggggattt ttgggcagta
     2041 agccgtataa cgaaatgctc ttgagcgatg gtgcggcccg cgcgcactac cggcgctatg
     2101 ccgaatggct taccgaacag ccgccggaac gactggcaca gaagcgcgcc gaggcggatg
     2161 ccctgtttca tcgcgtcggc atcaccttcg ccgtttacgg ccaagaggaa ggtaccgagc
     2221 ggctgatccc cttcgacatc gtgccgcgcg tcctgccgct ggaggaatgg attcggctgg
     2281 agaacggctt gaagcagcgc gtgcgcgcgc tcaatgcctt catccatgac atttaccacg
     2341 accagcagat tctcagagca aacgtcatcc ccgccgagca ggttctgtgc aactcgcagt
     2401 accggcccga gatgcaaggc gtggatgtcc ccgggggcat ttacgcgcac atcgccggca
     2461 tcgacatcgt gcgcgacgat gccggggagt ttcgcgtgct cgaagataac ttgcgcgtcc
     2521 cgtcgggcgt ctcctacatg ctcgagaacc gcaagatgat gatgcgcctc ttccccgagc
     2581 tcttctccgg gcaccgcgtg gcgccggtcg atcactaccc ggacgtgctg ctcgacaatc
     2641 ttcgcagcgt cgcacccact ggcgttgccg atcccaccgt ggttttattg acggcaggcg
     2701 cccacaacag cgcctacttc gagcatgcct ttctcgcgca gcaaatgggc atcgaactcg
     2761 tcgagggcca ggacctcttc gtgcgcgacg aagcggtgtt catgcgcacg acgcaaggcc
     2821 cgcagcgcgt cgatgtgatt tatcggcgca tcgacgatga ctttatggac cccttggtgt
     2881 ttcggcccga ttcgatgctc ggcgtgccgg gacttttgac cgcctaccgc gccggccacg
     2941 tcaacatcgc caatgccatc ggtaccggcg tcgccgacga caagtcgatt tacccctatg
     3001 tgccggacat gattcgtttt tatctcggtc aatcgcccat catcggcaac gtgcatacct
     3061 ggatgttgcg tcgcaaagaa gataaggact atgtactcgc gcacctgccc gaactcgtgg
     3121 tcaaagaagt ccacggcgcc ggcggctacg gcatgctcat cggtcctgcc gcgacgcagg
     3181 ccgagatcga tctgtttcgc aggcaaatca tcgcaacccc ggagcgttac atcgcacagc
     3241 ccaccttggc cctctcgacc tgtccgacgt tcgccgaccg caacctcgcg ccccgccaca
     3301 tcgacctgcg ccccttcgtc ttgtcggggc gcgatgtgac catcgttccg ggcggcctca
     3361 cccgcgtggc cttgcgcgag ggctcgttgg tcgtcaattc atcgcaaggc ggcggcacca
     3421 aggacacctg ggtgctggaa acctaatgct cagccgcacc gccgatcacc tctactggat
     3481 gtcgcgctac atcgaacgcg ccgagagcct ggcgcgcctg gtcgatgcgc actatcgcat
     3541 gtcgttgctg ccgcattcgg gcgaaaacct ggcgcaatcc ttgagctcga ccatgacggc
     3601 cttgcacatg gacgaaccct atcgcaagaa attcgacacc gtcgagccgc gcacggtgtt
     3661 tgagttcttg agcctggatc gggactacag cggcagcatt gtgagctgcc tgcgtgcggc
     3721 ccgcgaaaac gcccgcgcgg tgcgcggctc cttgacctcc gagctctggg agaccttgaa
     3781 ctccacctgg ctcgaggcgc gctcgctggt cgcgaacctg tcgccgagcc ccgatatcgg
     3841 ccacttcgtc gaatgggtca aggagcgctc gcacttaacg cgcggcgtca ccatcggcac
     3901 catgatgcgc gatgaagcct ttcacttcac ccgcataggc acctttctcg agcgcgccga
     3961 cagcaccgcg cgcattctca cggcgcatca aggcgacctc gtgcccggcg ccgatgccag
     4021 cgcagtgccc gatccgtatc agtggagcgt gctgctgcgc gccctgtccg cattcgaggt
     4081 ctatcggcgc gccttccgcg acgtgatcac gccgctgcgg gtggcgcagc tgctgatttt
     4141 gcgcgacgat atgccccggt cgctgctgcg ctgctgcaag gaggtgtacg ccaacttaaa
     4201 agcggtgtcg aacgaccaat cggcggagac tgagcggcgc gccggtgaaa tgcatgccat
     4261 gctgcacttc acgcgcatgg aggaaatcac ggccggcggc ctgccgcgtt tcctcgagca
     4321 attcctggcg cgcgtccgcg atctcggcga tcgcatcgca tcggatttcc tattaccccc
     4381 ggcagaggtt tgaagcgcat gcagatgcac attcgacacg aaacgcgcta ccgctacgag
     4441 cggcccgtca aatacagcgt acaaagtctg cacttgacgc cgcgtcgcga cttgagccag
     4501 cgcgcgctca cgtggagcat cacctcgccc gggcggcggc tcgagcaagt ggacgcctac
     4561 ggtaatatct cgcaccttct gaccatcgaa gagccgcacc gtgaaatcca aatcgtggtg
     4621 cgcggcgtgg ttgaaaccgt cgacaccgag ggccgccagg acgacggacc gctctcgccg
     4681 ctcgcctatc tcgctcctac tcaactgacg gcgccgaacg atgacctgcg ggcatttgcg
     4741 cagcaggcgc tgcacaccgt caccgaaccg cgggcacgcg cccaagcctt ggccgacgcg
     4801 gtgtttgaag cggtgcgcta caagtccggg accagcgatg tgcaggacag cgctgcggtc
     4861 gcctttcgca gcggcgaagg cgtttgccag gatcacgcgc atgtctacct ggcctcggcg
     4921 cgcgcggtcg gcattccggc gcgctacgtc agcggctact tgtacaccgg cgatgccagc
     4981 gatgcggcga gtcacgcctg ggtcgatgtg tggctcggca cggagatcgg ctggcagagc
     5041 atggatgtca cccacaagcg gcctgcggtc cgtacctact gccgcctcgc ggttggccgc
     5101 gattacctcg atgccgcccc ggtccgcggc gttcgccaag gcggtggcgg cgagaagatg
     5161 gaagccaatg tgctggtggc ggaatcggcc caacagcaat aaggtagaat ttcccgccat
     5221 gacgtactgc gtgggactgc tggtcgacac cggactcgtg atgttgtccg attcgcgcac
     5281 caacgccggt gtcgatcaaa tcaacacgtt ccgcaagatg gcgacgtttc agcgcgcgaa
     5341 cgaccgggtg ctggtgctca tgtccgcggg caatctcgcc atcacccagg ccgtcgtgaa
     5401 tttattgcac gagccgcctg ccgaggccga ccagccgacc cgcatctacc gcgccagcaa
     5461 catgttcaat gcggcgcgcg tggtgggcga tgcgctgcgt gaaattcacg aacgcgacgg
     5521 cgaatcgatg agcgagcacg gccatgactt caatggcacc ttcatcttgg gcggccagat
     5581 caagggcgag gagccgcggc tgtttcatat ctactccgcc ggcaacttca tcgagtcctc
     5641 gcccgatacc tcctacttcc agatcggcga atccaagtac ggcaagccca tcatcgatcg
     5701 cgtcatttcg cgcagcagca gcctcgcgca agcggcgaag tgcgccctcg tctccatgga
     5761 ttcgaccatc cgctccaatc tgtcggtggg cccgccgctc gatcttgccg tcattcggcg
     5821 cgacgaatat cggctggcga cgcatatcag catcgacatg gacaacgaat acttcaccct
     5881 gattcgcaag cgctggggct ttgcgctcaa ggaagtcttc agcgaactgc ccaatcccaa
     5941 ctggtagcga aggccgccga cgcgctcccc gcgtcagaga ttaccggcaa cgccgccagt
     6001 cgcagctttg gggcggagca atcgagccgc cacgccgccg agaagtccgg tcaccgcgga
     6061 tcctgacgtg ccgagcaaca agagcgtcgc aggcacatcg ccaagcagcc aggagacggc
     6121 gatccccgcg agggcgctca cgccgccggc gatgacaccg cccacggccg cgcctcccca
     6181 actgccccgc gctcgcagtt cataaatggc gccggcgatg cacgataatc cgagtccgcc
     6241 aaacatgaag gcgccctgca cagcgggtat ccaatgtccg gcgagcacca tgccgatctg
     6301 caggatggtt ccgatcagcg tcgccgtcac caacggttgc gtattcattt cggtctcctt
     6361 gtcattttga ttgtcttggt ttgtccgatt gctcttcggc cacccaatcc acggggccgt
     6421 gttcggccgc gcgcttcagg tgctccaacg tccacgagtc cccgaatcga aatttctgca
     6481 gcaaggcatc gaatgcgggg gttgccagaa accccacgcc gtccatctcc acttccgtcg
     6541 aatccttgcc gacggaccgc agattgatga ccgtcaccgt cttggcaaag tcttcggcat
     6601 gctcgaaacc cggcggtgct tgaatattgc gcagcacgag caggcgctcg ggcacgtagg
     6661 ccacaatgga attcttgata ttccccggtg caccgcgaac cgccgtcgcg ttgtagctcg
     6721 tctccaccaa cccgcccacc ccgaaatcaa cccacgccac cggcaccgcc caacttcgcc
     6781 agccctcggc actgatgaac agattccaga tcttgctgac cggcgcccgg atgcgaatgc
     6841 tgagttgcag cacgtgcgcg ccgttgggtt cggtgtagga cgcatcgcgc acgtcgggaa
     6901 agtcggcggc gggcgcgcgg ccgagcaatg cgccgacccc caagcacagc agcgtgccac
     6961 tgcgcaggtt catgaagttg cctggcgcga ggcaacgcgc cgcaaagaat tcatatagtc
     7021 acgcaacgag gccaccgccg catcgaaggc cttcagattc cgataggtgc gcgactgcat
     7081 cacgccgcct tccatcaccg tcaacacgaa actcgccacg cgtcgcgtat cgacgtcggg
     7141 tccgagcgtc gctcgtgcct cgatcaggca gcgttccacg tgatccaccc agccggtgaa
     7201 gttatcggcg aggagcttgc gcacggcagg gtccggctca tggatttcca aggccaaact
     7261 tccgatggga cacccgtagg cgcaatcggt ggtgcgcaac aacatccgat agcgcccgag
     7321 cagggcaaaa atgcgttcga tcggatcatg cacattcttc caggccggct cgatcaacat
     7381 cggcacgatt cccagccgat accgctcgag caccgcgagc aggaggtcct gtttggtcgg
     7441 aaagaaatgg tagaagctgc cgctgtggca tttggactcc tgcaacaaat cggccacgct
     7501 ggtggactga tagcccttct cggcaaacaa tcgcagcgcg ctcgtgatga gccgctcgcg
     7561 ggtcgattcg tcctccattg cacctcactt gatcgatcaa tcaaatcacg agttgagtga
     7621 acaatcaagt cctccaatag cggtacttgg tcacagaaga acacctgctg gaagcggccg
     7681 tttgcggctg acggtaagct ggctgagtgt tcgctgagga cacgttgccg ccgcctgcta
     7741 gcggtgaggg aaacgcaatg acggatgagt acgatgaccg ggtcccgtcg ccgttgttgc
     7801 ggtgtcttgg gatcgtgggg gcggcgttct tgttcctgta tggcgtcgta ggcgtgttgc
     7861 ataacgatct aaaagtcgcc catgcgaaat ccggtcccgg cgtgcacctt cacgggcttc
     7921 tcgcgtgggt ttgctttgcg ggaatggtga tgatgtcgat cggcggcatt cgctttctcg
     7981 cccctggtga tggcaaattc gatttttccg gacgtcggcg ccgattcggt ccactcatcg
     8041 tggtcgggct ggtgttgtac cttgccgcgc aggcgattgc cggcctgcgg tcatgacgct
     8101 aaacccaacg ctggttgacc aagcacccgg agcctgcgct cggacgtgtc gtgatcggcc
     8161 gactactggt aaaaagcgcg cgcggcgcgc aatagtcgac gcaccacgga aaggcaagaa
     8221 agccatgaac atcaaaatct tcattgtgac gttactggca tcgttcgccg cgcaggcagg
     8281 ggactggacg gatatgcttg ccgacttgat gcaacaaccg gacaccagga tcgaattcat
     8341 ccaatcaatg agagatctca tcaaggctga cgggtatgtc tgctacgctc ctcggcacat
     8401 acgatccgtt gattcggcgc tgtatgtcgt cgagtgtgat cacgaagcgg atggcgccac
     8461 atcgctctat agcgtgagcg ccacccccat ggggaacggc ctcgtctggc acgccacgcc
     8521 gcgccagtca tgaacctcaa acgctaattg gccgccgcgg atccgctctg gcgcggcttc
     8581 tcgtcatgga cacagatggt ctcggtcaag cgggggccgg gtgcatacga atggccgctc
     8641 gcgctcgcga cctgatagca gtcggaattg acccacacga tccaatcgcc gctcggcgtc
     8701 ttgaattgct cgccggcgtg atgacgggtg cgcggccacc aattctcctc cgccggcgac
     8761 cccgtcaata gactatcggg cggttgattt gtgcggattt cgtaagcctg cacggcgcgg
     8821 cgcgcctccg ctttccaatc aacccccgcg ccgctccccg catcggcacc agaggcggac
     8881 agcggcggcg cggcggaagc gagcaccggc gcagtcaccg aaatcgcatg atcgccgttg
     8941 aagcctcgct tgcgagaact gtcggcgcgt attctcggca tcgtgttcgg cgggaagaat
     9001 tgcacttcca ccggatcatc catcaggcgg gcgatctgtt cggttcgcga caccatgaag
     9061 agggcggcca gcaacgcacc atgaatcagc aacacgccaa cgagcgtgag gtagcgcggc
     9121 agcccccatc tcgttgagta gtccatgtcc atgtcgcttg agttaccaac gttcttgacc
     9181 cgtcacaccg gacttggttc ctcgacggcg gcgcccgctt tccccgctga cttccacgaa
     9241 agctcctgca aggtcggtac cagccaaatc cagccgatga gcccaaacag gctcatggcg
     9301 cccgccgcga cgaacgcgta cgtgtaatgc ccggtgatct gaatgatgaa cccggtgatc
     9361 gtgggcgccg cgacgccggc gaggctgcca atcgaattct ccacgcccac ccagcggccc
     9421 gaggcgcgcg gaccggcgag gatctggcac atggcgtaga cgccgggcga ggaggcgccg
     9481 cacaaaatct ggtagatgaa catgccggcc aacatcagcg tcggggaacc cgccgccatg
     9541 aaccacatgc agggcacata gccgatatgc gcgatggcca tcaccgattt gtatccgaca
     9601 ttcggcgaac cgccgcgcgc gatgtagcga tcgatggccc aaccgccgag gaacgccgcc
     9661 gcggcgccca ccgtgtaggc cagggtcgcg agctgcgtca tgcggaccag cgacatgccg
     9721 cgctcggttt gcaaatacga cggcagccaa ttgagcatga agtagaacaa gtagttgctc
     9781 gagaatagcc cgagccccgt gccccacagc gccggctgcc tgagaatcat gccccaggtc
     9841 ggcgagtctt cgttgggctt gggcttgagc gccgtgcgca cctcacccgc cacgcgcgac
     9901 cacggcaaca gccacagcag cgacagtgca ccgaacacca ggaacgagga gcgccagccg
     9961 aaattcgcca tcagcatgcc gcccgcatac gtgcctaccg cgggcccgac caggtaggca
    10021 agcgcgacga tgccgttggc ggtgcccagg cccttcgggg gaaccacggc cgccaagatc
    10081 ttcgccacgc tcggaaaacc cgaactctcg cccagaccca acagcagccg cagcaccagc
    10141 aagcttgcga acgtgtgggc gatgcccgtc aacatggtgg cggatgccca gatggtcaga
    10201 ccggccgcga gaatgcgatg cgggccgaag cgctcggcca gccaccccac cggaatctgc
    10261 atgcaggtgt aggtccagta gaaggcaccg aacagcacgc ccatctcggc ggcattcaag
    10321 tgcaggtcgg cctgcatcag gtgcgcggcg gtgggcatgg tgccgcgatc gacgtaattg
    10381 atgaacagca ccgcggccag gagaaaaacc acgggactgc gcgcggcgcg cgcggaatcg
    10441 tttgctgctg acgcgggcgg gttcagctcg cgtaatccga atgagcgcga tcgagaacgc
    10501 gcttcaacgc atcgaaatgc aggtcggcgc ggtcccgacc gccgaccgtg gcgcccgcgc
    10561 cgaattgtgc aaccgtcttc tcgatcagcg catccgggat cagccgcaac ggccgcccgg
    10621 tggagagcgc cagaacctgc gcctgagcgg cgcgctcgag aaaatacaag cgctcgaagg
    10681 cgagcggaat cgtcttgccg accacgagca cgccatgatt ggccatcatc aggaccgact
    10741 tgtcgccgag tgcggccgcc aaacgctcac cctcggtgag gtcgaaggcg agaccgttgt
    10801 aggtaggatc gtaggccacg tcattgtaga agccgagcgc actctggacc gccatttcca
    10861 gccgcggatt ttgcagcatg cagagcgccg tcgcatacgg catgtgggtg tgcagcacgc
    10921 agcggccctg cggtgacagc cggtgcacgg gctgatggat gtagagcgcc gtatcctcca
    10981 ccgcaccctt gccgctcaac acctcaccgt cgaaaccgac gacgagaaaa tcgctggcgc
    11041 ggatctcgga ccaatgcatg ccgaacggcg cgagaaaaaa tcgatccgca tatcccggca
    11101 ccagcagcgt caggtgatta tcgatgccct cattgagacc gaagcgatcc gccagccggt
    11161 aacaggccgc gagatcgacg cgcgcgcggc gctcgacatc ggccgcgggt tcaacgggtt
    11221 ttagactggc attcatgggg ttcgcctcgc tgtgaaatcg tacgcctagt gcagcaactg
    11281 atccagcacc ggcagcggca cttcaccttc gcgcaacgcc gcctcgtgga actgcttgag
    11341 actgaaggcc gcaccgtgtt tggcctggta atgctcgcgg tcagcgagcc agcccttgta
    11401 gccggcatag taggtcggca gctggcacga cgcgagcttg gcgcgctgca acttgccgct
    11461 cgcctcttcc atttcctgat aagtgctctt ggtcatgaga tcgagcgcct gctggtcggt
    11521 catccccaag gtctgcaggc gcacgtccaa aatcgtattc gccagcacgc gcagcaattg
    11581 cttttgcagc gtcaggcgat agcccggcgt gtcgcccaag tagccctgct cggccatcag
    11641 ctgctgcgcg tacaccgccc agccctcgat gtacgggccg ttgccgaaga tattgcgcag
    11701 caagcgccgc gagcgcggtt gcacatcatt cgcatactcg gcttgcacat agtgcccggg
    11761 catcgcctca tgcacggtca gatgctgcat gcccgatgca ttgtattcgc gcagtttcga
    11821 gtcgacgcgc gtctgcggcc aatcgctcgg aatcggcgtc acccagaaaa aagcgccgag
    11881 cttcggttcc aacgccggcg ccgggttgaa gccgccgacg ctgtatatcc cgcgcatgaa
    11941 caccggcgtg tcgatcactt ccaaattggc gttcggcggc aaggtgagca gatccttcgc
    12001 cttgacgaag gccgtggcgc ccgccaatgc ctgcttggcg gaggccatga aacttgccgc
    12061 cgtcgcatgt tggcgcgcca catccgccag cgcttcttcg accgttttcg gcgccgccaa
    12121 gcgcgcgatt tccgcccgca ccttgacgag gtccgcctcc gcttcggcga gcagcgcttc
    12181 cggagtcttg ccggtcgcca aggtgtagcg gaacttcttg gcatagcgct ccttgcccag
    12241 gcgccaatcg ctcttgcgct ccgataaatt gttctccagc cagtgattga agctctgcag
    12301 ggatgccatg gccgcggccg ccgcctggtc atagcgcatg cgcgcgtccg gcgggcaatc
    12361 ggcgcgcagc gtcgtgtcga tgagtccgac gttgccgtcg ttctcttcgc gcgccacgcg
    12421 attccacacc tcgggggaat cttgcaagtt cgtctcggcc tgctggatca gctcgggcac
    12481 cttttgcagc cggctgatga tgtgccggta gcgctcgggt gccggcgcgt aatggagcac
    12541 atagggcgag tacaagccgt tgcccaacag ctcgacatag gtcgtcggat tatggcgata
    12601 gctctgaatc tcatcgagct ccaagcgcgt cgcgccgagc gcgttgcgca tgatgtcgac
    12661 atcggcgcgc tgttcggggt cgagcgccgc accgtcgaaa tgcccgattc gaatctcgat
    12721 gtcgtgcaga aaactgcgct gcgcctcgat gcccgccgca ctgaagtcat ccagctgatc
    12781 atcgagcgat acgccgtggt gcacgtggta gccgaatccg gtcgcgccgg tcggcgacag
    12841 cgccaacgtg cccatgacga agtcatccac caattggtcg aatggattat cgccgctcgc
    12901 gggctttaca ttcgcagccg ccgcccaagc ctgcgcgcac atcccggcga ggcataaaag
    12961 gccgccgagc ccgctcatca tcccagcgct tttgattttg ttcatcgccc attttcggcc
    13021 aactcgcctc ccggcgtcca gccattcgaa gtctttgggt taagatgcca ggccatgccg
    13081 ttgcatcagc atccatgaaa ttgcgcaaga tcattcgcgg cacggtcatc gtgctcgcgc
    13141 tcctactgct ggtgttgatc gcggcgctct ggctcaaacc cccggagctg ctgcggatcg
    13201 gcgccaatta cgccgcgaaa atcgtctgct cgaacgtgtt tctcgccgga cgcgatccgg
    13261 aggaagtgct gcgcaccgat gtgcaagccc ccggcgcggc gctgctgcat ctcatgcgcg
    13321 tctcggtcga ccggcaacaa caggtggtgc gcaccggact gttgggtttc atcggcgacg
    13381 gcgtggcact ggcgcggccg gacaccggct gcgccgtggt cgccgacggc aacgtggctt
    13441 acgcacggca aatcggcgcg ctcgccgcag ccggcctccc gacgcccgac gccgcatcga
    13501 cggatagccc cccgggtccg aattcgggca cggccgcgac caccgctgcg ctgtggccgg
    13561 acggcgatgc ggtcacgccc aacccggcgc tcgatcgcgt gctcgccgac gacacgctcg
    13621 caggtcccgg cgtacgcgcc atcgtagtcg tagatcacgg ccgcattgtg gccgagcgct
    13681 acggcgccgg tttttcggcg aagacgccgc tcctggggtg gtcgatgacc aaaaccgtcc
    13741 tcgccggcgt cgtcggcgcg ctcatcaagg acgggcggct caccctcgat caggccgcct
    13801 tctggccgaa ggccgccggc gatgcgcgcg atcgcattcg gatcgcggac ctcctcgcca
    13861 tgtcgagcgg cctgcgcttc aacgaaggct acggcgccgt gtcggatgtg acgcgcatgc
    13921 tctacctaca gccggacatg ccggggttcg cacacgcgca gcccctcgag cacccgccgg
    13981 gcgggtattg gagctattcg agcggctcgg ccaacatctt gtcgcgcatc gcccaggatg
    14041 cagcgggccc ggtgggcgcg aattatccgc gcgagaaact cttcaagccg ctcggcatga
    14101 cgagcgccac catggagacc gacgaagacg ggaccctggt cggttcttct tacatgtacg
    14161 ccaccgcgcg agactgggcg cgctatggat tgttcctgat gcaagcaggc gtttggcacg
    14221 gccaagcaat gctcccggcc ggctacgtcg ccctgatggc ctcacccgtg tcggcctccg
    14281 gtggacagta cggtcacggc ctggtctggt tgtggggttc ggacggcaag ggcccgggga
    14341 tgaacccgga tagcgcattc ggcattcccg ccgatacgtt ttggatgatg ggccatgacg
    14401 gccaaagcac cgccattatc gcctcgcgag gacttgtggt ggtgcgcctc ggattgacgc
    14461 cgtggcagga tcgctaccag ccccagcctc tgatcaaggc cgtactcgca gcgctgccgt
    14521 agcggcgctt cgccggcgcg acagatagag gtacaccggc acgctggcca tgagcatgat
    14581 ccccgcgatc agcgcggacc tcgggtcttc cacgattgcg acgatccaca acgatacgca
    14641 gcccaagagc acgatggcgg tggtgtaggg atagccgaac gcgcggtacg ggcgcggcgt
    14701 ctcgggctcg cgccggcgca gcacgaacaa cgcactatag gcggacacgt acagcagcaa
    14761 gaacagcacg gcagccaccg cgacgatctg ttcaaaagtg ccgctcatga tgagcgccgc
    14821 cgcacccacg ctcgagatcg tcaaggcgac gcgcggagtc ccgccggcgc tgaccgcggc
    14881 cgccttttcg gtgaacaagc catcgcggcc gatggcgaac agaatgcgcg gcgccatcag
    14941 aatcgtggcg ttgatcaggc tcaacaccgt gacgagagaa atcagcgtga cgagttcggc
    15001 accgccgcgc ggcaatacca cccgggcgac atccgccgcc ggcagcttcg aggcggcgag
    15061 taccgacaac ggcagcactc gcaggatggc gatattgatg atcacgtaca ggacggtcac
    15121 gagcac
//
LOCUS       DASWZT010000155         1751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_59218, whole genome shotgun sequence.
ACCESSION   DASWZT010000155 DASWZT010000000
VERSION     DASWZT010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59218"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VGE92_04275"
     CDS             complement(<1..473)
                     /locus_tag="VGE92_04275"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY1313066.1"
                     /translation="MKAAASARAAPSRHGLVLGAAGMALCVAACGGRESAPPASEPAV
                     LNIYNWADYIGRDTIAEFERRTHIKVVYELYDSNQTLEAKMLAGRSGYDIVSTTTAFY
                     ERQIKAGAYLALDRSQLSNWNNLDPAVLAIQAAADPGNRYAVPYLHAMNGLAYNV"
     gene            complement(485..1654)
                     /locus_tag="VGE92_04280"
     CDS             complement(485..1654)
                     /locus_tag="VGE92_04280"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY1313067.1"
                     /translation="MQSTSDSVAAGYNGLTEHTHGLPADAYFDPRQYQRELQRIWYRN
                     WVYVGRSSDVARARAFRSFELGDQKLLLVRDDQGTLQGFHNTCRHRGAALCRETEGVL
                     RTGSIICPYHAWVYNLQGDLLRTSSKAHADGFDVAAYPLYKISVKEWNGFIFVALSAA
                     PPPFEKSFDVPLNRLDAWPLAELFVGHALRKTIRSNWKIFWENYNECLHCPGVHPQLS
                     QLVPIFGRGLLEERDDPEWRAHALDPDPKFKGGLRSGAATWSLDGKASGAPFPAIADA
                     DRKVGHVYMTGLPSVFLVGHIDYVRVVRLLPLGPELCELRVEYLFSPQTLADPQFDLK
                     NVVDFTNLVMTEDAEVCELNQLGLHAGPHARGVVMPEEYVIRQLHDWLRTELGRV"
BASE COUNT          319 a          557 c          540 g          335 t
ORIGIN      
        1 tccacgttgt aggccaagcc gttcatcgcg tgcagatagg gtacggcgta gcgattgccc
       61 ggatctgccg cggcttgaat cgccaacacc gccggatcca gattgttcca gttcgacagt
      121 tgcgagcggt cgagcgccag gtaggccccg gccttgatct ggcgctcgta gaacgcggtg
      181 gtggtgctga cgatgtcgta ccctgaacgg cccgccaaca tcttcgcttc cagcgtttga
      241 ttcgaatcgt agagttcgta gaccactttg atgtgcgtgc gccgctcgaa ttctgcgatc
      301 gtgtcgcggc cgatataatc ggcccagttg tagatgttca ggaccgcagg ttcgcttgcc
      361 ggtggcgcag actcgcggcc gccgcacgca gccacacaca gcgccatgcc ggccgcgccc
      421 agcaccagac cgtgccggga cggcgctgcg cgcgcactgg ccgcggcttt caccccgctc
      481 gtccctagac gcgtcccaat tcggtccgca gccaatcatg cagttggcgg atgacgtact
      541 cctccggcat caccacgccg cgcgcatgcg gccccgcatg caatcccagt tggttgagtt
      601 cgcacacctc tgcatcttcg gtcatgacca gattggtgaa gtcgaccaca ttcttcagat
      661 cgaattgcgg atccgcgagc gtctgcggcg aaaacagata ctcgacgcgc agctcgcaaa
      721 gctcggggcc caacggcagc aagcgcacca cgcgcacgta atcgatatgc cccaccagga
      781 acaccgacgg caggccggtc atgtatacgt gaccgacttt tcgatcggcg tcggcaatag
      841 cggggaacgg cgcgccgctc gccttgccat cgagggacca ggtcgcggcg ccgctgcgca
      901 aaccgccctt gaatttcgga tcgggatcca gggcgtgagc gcgccactcg ggatcgtcgc
      961 gctcctcgag caaaccgcgc ccgaagatcg gcaccagctg cgagagctgc ggatgcaccc
     1021 ccgggcagtg caggcactcg ttgtagtttt cccagaatat cttccaattg ctgcgaatgg
     1081 ttttgcgcaa ggcatggccg acaaagagtt ccgcgagcgg ccatgcatcg agacggttca
     1141 gcggcacgtc gaagcttttc tcgaagggcg gcggcgcagc gctcagggca acgaagatga
     1201 acccgttcca ttccttgacg ctgattttgt agagcgggta agcggcgaca tcgaagccgt
     1261 cggcgtgcgc cttcgacgag gtgcgcagca aatccccttg caagttatag acccacgcgt
     1321 ggtacgggca gatgatgctg ccggtgcgca gcacgccctc ggtttcacgg cacagcgccg
     1381 cgccacgatg ccggcaggtg ttgtggaatc cttgcagagt gccctgatcg tcgcgaacca
     1441 acagcagttt ttggtcgccg agctcgaagc tgcggaatgc ccgcgctcgt gcgacatcgc
     1501 tcgatcggcc gacatatacc cagtttcgat accaaatccg ctgcagttcc ctctggtact
     1561 gccgcggatc gaagtaggcg tcggccggca ggccatgggt gtgttcggtt agaccgttat
     1621 agcctgctgc gacgctgtcg gatgtgcttt gcatggttgc ccccgccgcc gaatatactt
     1681 catgtgcgca ttaattggcc gctcggatcg aatggcgggg ccgcgagctt gagtgccggc
     1741 cggcgctcgc c
//
LOCUS       DASWZT010000156         1921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_54419, whole genome shotgun sequence.
ACCESSION   DASWZT010000156 DASWZT010000000
VERSION     DASWZT010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54419"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VGE92_04285"
     CDS             <1..781
                     /locus_tag="VGE92_04285"
                     /inference="COORDINATES: protein motif:HMM:NF016190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEY1313068.1"
                     /translation="CKASVTNGAVDWVVCDVQAATFAGYSADSSLGSAAPTVAEGIAT
                     LTSTDKDFSMPALAARVKTAFLALQDAWCNQKLDAGRAFMSPGAYFSWSAQLESMASE
                     GRRNVLEHLEVRSIEPIRVIHGRVFDDLTVRITAVAADFEVDGSGRIVFGDRSVQPFK
                     EDWTFQRSVGVATSNKPGTLENTCPSCGAPVSLTQLGECRYCKAAVTSGRFDWVVSRI
                     DQEDGSGGSATAADRIGEQMALQIGGAVIGGLLSSLLSDRR"
     gene            complement(786..>1921)
                     /locus_tag="VGE92_04290"
     CDS             complement(786..>1921)
                     /locus_tag="VGE92_04290"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HEY1313069.1"
                     /translation="AEAAWRDCLKLEPRLIDAHNNLAKLIWMRTGDIAQTIAPLDQAL
                     RSFANDDALWAAKAAILQGAGDARGAFQCLSRQAASAQAQPMLLVRAGLAALEFDSAA
                     AVTLAERALRAAPQNAAARSLLAAALLGVGEAHAALAHCDALLTQAPDDQYLIALKTT
                     AWRLLGDAGYAALCDYQNLVLPLELEAPTAWPDLASFLADLNVALNRLHDPHGHPLLF
                     QSLRHGTETTQDLARSTDPTIQALFASFAAPIRRYLEKVGPGADPLRRRNHGRWRFNG
                     SWSVRLRSAGHHTNHVHPRGWLSSACYIALPDSMNDGNTTDGILTFGEPGIATAPTLA
                     SEYSVRPRVGMLVLFPSYFWHGTLPFRSAQPRLTVAFDVVPDA"
BASE COUNT          332 a          582 c          679 g          328 t
ORIGIN      
        1 ctgtaaggcc agcgtcacca acggcgcggt ggactgggtg gtttgcgatg tccaagcggc
       61 gactttcgcg ggatattcgg ccgattcttc gctcggcagc gccgcgccga ccgttgccga
      121 gggcatcgcc acgctcacgt cgaccgacaa ggatttctcg atgccggcgc ttgcagcgcg
      181 ggtgaagacc gcgttcctgg cgctgcagga tgcctggtgc aatcagaagc tcgatgccgg
      241 ccgcgccttc atgagtcccg gggcctactt ctcctggagc gcgcaattgg agagcatggc
      301 gagcgaaggg cgccgcaacg tgttggagca tctcgaggtt cgcagcatcg aaccgatccg
      361 ggtgatccat ggccgagtgt tcgatgattt gaccgtgcgg atcacggcgg tcgccgccga
      421 cttcgaggtg gacggcagtg gtcggatcgt ctttggcgac cgcagcgtgc agccgttcaa
      481 ggaggactgg acattccagc gatccgtcgg cgttgcaacg tcgaacaaac ccggaactct
      541 cgaaaatact tgccccagct gcggcgcgcc ggtgtccttg acccagctcg gagaatgccg
      601 atactgcaag gcggccgtca ccagcgggag attcgactgg gtggtgtcgc gcatcgatca
      661 ggaggacggc tccggcggca gcgctacggc cgctgatcga atcggcgaac aaatggcgct
      721 gcagatcggc ggcgcggtca tcggaggctt gctcagcagt ctgttgtcgg accggcgctg
      781 atcgctcaag cgtcgggtac cacatcgaac gccacggtga ggcgcggctg tgcgctgcga
      841 aagggtagcg tcccgtgcca gaaataggac ggaaatagca ccagcatgcc gacgcgcggg
      901 cgcaccgagt actcgctggc gagcgtcggc gcggtcgcaa tgccgggctc gccgaatgtc
      961 agaatgccgt cggtggtatt gccatcgttc atgctgtcgg ggagtgcgat atagcatgcc
     1021 gaggaaagcc agccgcgcgg gtgcacatgg ttggtgtggt gaccggcgct gcgcaagcga
     1081 accgaccaac tgccattgaa gcgccagcgg ccgtgattgc gacggcgcag gggatctgcg
     1141 cccggaccca ccttctcgag gtagcggcgg atcggcgcgg caaagcttgc gaatagcgcc
     1201 tggatcgtgg gatcggtgct tcgcgccagg tcctgcgtcg tttcggtacc gtgccgcagc
     1261 gattgaaaca acagtggatg accgtgcggg tcgtgcagtc gattcagagc gacgttcagg
     1321 tcggccaaaa agctcgccaa gtccggccat gccgtgggcg cttcgagctc gagcggcaac
     1381 accagatttt ggtagtcgca gagcgctgcg tagccggcgt cgccgagcag ccgccaggct
     1441 gtcgtcttga gggcgatgag atattggtca tcgggcgctt gggtcagcaa cgcatcgcag
     1501 tgggcgaggg cggcgtgtgc ctcgccaacc cccaagaggg cggcagcgag caagctgcgc
     1561 gcggcggcgt tctgcggcgc cgcgcgcagc gcccgctcgg cgagagtcac ggccgccgcc
     1621 gagtcgaatt ccaaggccgc gagtcccgcg cgcaccagga gcatgggctg tgcttgggcg
     1681 ctcgcggcct ggcgggacaa acattgaaag gcgccgcgcg cgtcgcccgc tccctgcaga
     1741 atagccgcct tggcggccca tagggcgtcg tcgttggcga acgagcgcag cgcttgatcg
     1801 agcggcgcta tcgtctgcgc gatatcgccg gtacgcatcc aaatcaattt cgcgaggttg
     1861 ttgtgcgcgt cgatcaatcg cggctcgagc ttgagacagt cgcgccaggc cgcctcggcg
     1921 t
//
LOCUS       DASWZT010000157         1841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_64096, whole genome shotgun sequence.
ACCESSION   DASWZT010000157 DASWZT010000000
VERSION     DASWZT010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64096"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..967)
                     /locus_tag="VGE92_04295"
     CDS             complement(143..967)
                     /locus_tag="VGE92_04295"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY1313070.1"
                     /translation="MRMLRNRALTALLLACAAGCAGNGKGLDQNGNPVSSSDGTSGPI
                     SADFQSIQDNVFTPICSKCHIGAGAPEGLQLDAAHSYNLLVGVPSNEQPSVLRVKPGD
                     PDDSYMVRKIEGEAGISGGQMPFGETPLPQATIDAIRQWITNGAPNAGTAARTFALET
                     TAPVDQATVNAPLARVVVTFTQEVDASLVNETTVVVEKIADLEPGATSHSVSVASTRA
                     LAADNAAVLLITPRVPLDPGVYRVTLRGTGGAALANLNAATLGVNSSFEFTVEPAP"
     gene            1096..1821
                     /locus_tag="VGE92_04300"
     CDS             1096..1821
                     /locus_tag="VGE92_04300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEY1313071.1"
                     /translation="MTLRPFACGAACSLALVLQTPQSFAADVWGGSLSVTSDYFVRGI
                     SRTADRAALQLDLHYLNSSGFVAGLFASNADVDPDLPSDVELDAFVGFAWTAGSDWRG
                     RILASQYAYPWNKLGSSYDYDELNLEMGYEDWLGVALTYSPNAPRYLPHSGLIDVSSE
                     AAEVNLQHALLPHLSASGGIGYAYLSGPEAAGYGYWSIGAAYELAPVTLVLSYVNTTA
                     AAKTLFYNAAADGRWTGTIIWRF"
BASE COUNT          323 a          565 c          582 g          371 t
ORIGIN      
        1 ccgccgccgg tcggattgac gtgacactgc ccgcacttga gcccgagctg taccgcgagg
       61 tacggttcgg cgtgcgcccg gaccgcggcg aagcacagca gcagtagcag agggcgcacg
      121 gcaaatcgag cgagcgcatc gatcatggtg caggctccac ggtgaactca aatgagctat
      181 tcacgccgag cgtcgcggcg ttcaagttgg caagtgccgc gccaccggtg ccgcgcaacg
      241 tcacgcgata cacacccgga tcgagcggca cacgcggagt tatcaacagg acggcggcat
      301 tgtccgccgc cagcgcgcgc gtgctcgcaa cgctgaccga gtgcgacgtt gcacccggct
      361 cgaggtctgc gattttctcc actacgaccg tggtttcatt gaccagcgaa gcatcgacct
      421 cctgggtgaa cgtgaccacg acgcgtgcca gcggtgcgtt cacggtcgcc tgatcgacag
      481 gcgccgtggt ctcgagagca aacgtacgcg cagccgttcc cgcattgggc gcgccgttcg
      541 tgatccattg acggatggcg tcgatggtgg cctgcggcag cggcgtctcg ccgaacggca
      601 tttgaccgcc gctgatgccg gcttcgccct cgatcttgcg caccatgtag ctgtcgtcag
      661 gatcgccggg tttcacccgg agtacgctcg gctgttcgtt gctcggtacg cctaccagca
      721 ggttgtagct gtgtgcggca tcgagttgca ggccttccgg cgcgcccgct ccaatgtggc
      781 atttcgaaca gattggcgtg aaaacgttgt cctggatcga ctgaaaatcc gcgctgatcg
      841 gtcctgaggt cccgtccgag gaggagactg ggttgccgtt ctggtcgaga cctttgccgt
      901 tgccggcgca gcccgcggcg cacgccagca gcagcgccgt caatgcgcgg tttcgtaaca
      961 tcctcatact cgacgcctcg atggcaacgc cgaggagtaa gtaccggcac gcgggcgacg
     1021 ggtttacgga tgccccaaga tttcgagaaa ggtttaatct gtcgccatgc atgccccgac
     1081 gagaatttgg cggcagtgac gctgcgtcca ttcgcttgcg gcgcagcgtg ttcgctcgct
     1141 ctcgtactgc aaacgccgca aagtttcgcg gcggacgttt ggggcggctc gctgtcggtt
     1201 accagcgact atttcgtgcg cggcatttcg cgtaccgccg atcgcgcggc actgcagctg
     1261 gatctgcatt acctcaacag cagcggattc gtggcgggcc tatttgcgtc caatgcggac
     1321 gtcgatccgg atctgccgag cgatgtcgag ctcgatgcat tcgtgggttt tgcatggacc
     1381 gccgggagcg attggcgcgg caggattctc gccagccagt atgcctatcc ttggaacaaa
     1441 ctcggttcga gctatgatta tgacgaattg aaccttgaaa tggggtatga ggactggctc
     1501 ggcgtggcgc tcacctattc gccgaacgcg ccgcgctatc ttccccacag tggactcatc
     1561 gatgtctcct cggaggcggc cgaagtgaac ctgcagcacg cgctgctgcc gcacctatcg
     1621 gcaagcggcg ggatcggcta tgcctacctg tcaggtcccg aagcggcggg ttacggttat
     1681 tggagcattg gtgccgccta cgagctggcg cctgtgaccc tggtgctgtc ctatgtcaac
     1741 accacagcag ccgctaagac attgttttat aatgccgccg cggatggccg ctggaccgga
     1801 acgataattt ggcgcttttg atttccgcca ctgcacggca a
//
LOCUS       DASWZT010000158         3017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_1163, whole genome shotgun sequence.
ACCESSION   DASWZT010000158 DASWZT010000000
VERSION     DASWZT010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..842)
                     /gene="phoB"
                     /locus_tag="VGE92_04305"
     CDS             complement(147..842)
                     /gene="phoB"
                     /locus_tag="VGE92_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEY1313072.1"
                     /translation="MTAKRILIVEDEKAIRDMVAFGLRRAGYEVREAEDCREARARIV
                     DVRPDLMLIDWMLPDMSGLELTRSLKRAKDTEEIPVIMLTARAEEQDKVGGLEGGADD
                     YITKPFSPRELLARIQAVLRRAAPAGVADVVEADGLKLDDSSHRVSAGDREIALGPTE
                     YRLLHFFMTHPERVFSRGQLLDRVWGGNTYVEERTVDVHIRRLRKALEPYLLERLVQT
                     VRGAGYRFSTRDA"
     gene            complement(913..1602)
                     /gene="tsaB"
                     /locus_tag="VGE92_04310"
     CDS             complement(913..1602)
                     /gene="tsaB"
                     /locus_tag="VGE92_04310"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEY1313073.1"
                     /translation="MRVLALDAATEACSVALLNGDELTHRMMHGGRCEASQILDMVDA
                     LLAEAGLTLAALDGIAASVGPGAFTGVRIGVAVAQGLAFGSGLPVAAVTTLEALAMPL
                     FRAGATRALACLDARMGEVYWGCFAADPTEGLLAAGAARVGPPESVALPTDGSYQGVG
                     RGFAAYPLLARLPGLTVAAEASDALPSAREFARLGALRLGLGDGLDPGELCPVYLRDK
                     VALTSAERALK"
     gene            complement(1606..>3017)
                     /locus_tag="VGE92_04315"
     CDS             complement(1606..>3017)
                     /locus_tag="VGE92_04315"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY1313074.1"
                     /translation="HVVEARRTAQAADIVVVNHHLLLADLALKDEGFGDLLPGAEAVI
                     LDEAHQIPDIAAQFFGQLWSVRQVQLLLRDIAAELTAGSVYAPAITECAAGVDTCIEA
                     LRSSLQGAPGRIEWASLPESFLDALPELETAISDLATRLEGLGAGAGLANCARRAAVM
                     ANSLAALRELPDDEGLRWVDANASGMLLQLTPFEIAQRLRQYVEARPCAWIFTSATLA
                     IGEDFSHFAARVGMPEARTVHIDSPFDYADQARIFLPPHMPQPQDPAFAARFIDACAP
                     LLEASGGRAFLLYTSYRGLAEGVRALHARFPNPTFPVLVQGEAPREALLNRFRELGNA
                     VLLATGSFWEGVDVKGEALSIVAIDKLPFASPDDPLLKARLEGIRRRGGNPFFEYQLP
                     QAVLALKQGVGRLIRDFDDFGVIVVGDPRLRTKGYGRVFLQALPPSPVITDGAAGARF
                     LVERLAKLRPARALASQVT"
BASE COUNT          581 a         1036 c          921 g          479 t
ORIGIN      
        1 tccgaggtag gagcaggcgg ccgccaacaa ccacacccaa atgggcccga tcaggaggcc
       61 caggacgagc cccgaaagca ggatgcccgc gagccgcgcg agcgcgaaag tccacatcgc
      121 tgccattgac tagttgctgg ccgtcctcaa gcgtctcgtg tggagaaccg gtaaccggcg
      181 ccacgcacag tttgcaccaa tctttccagc agataaggct cgagtgcctt acgcaaccgg
      241 cgaatgtgca cgtccactgt acgctcttcg acataagtat tgccgcccca gacccggtcg
      301 agcaactgac cgcgggagaa aaccctctcc gggtgggtca tgaagaaatg cagcaaccga
      361 tactcggtgg ggccaagggc gatttcgcga tcgccggcgc tcacccggtg gctcgagtca
      421 tcgagtttca gaccatcggc ttcgacgacg tcggccacgc ccgccggtgc cgcgcgtcgc
      481 agtacggctt gaatgcgcgc cagcaattcg cgcggcgaaa acggcttggt aatgtagtca
      541 tcggcacccc cctcgaggcc gccgaccttg tcctgctcct cggcccgcgc ggtcagcatg
      601 atgaccggta tttcttcggt gtccttggcg cgtttgagcg atcgggtgag ctcgaggccg
      661 ctcatgtccg gcaacatcca atcgatcaac atcaaatctg ggcgcacatc gacgattcgc
      721 gcgcgcgcct cccggcaatc ctcggcttct cgcacctcgt agccggcacg gcgcagaccg
      781 aaggcgacca tatcgcgaat cgccttttcg tcttcgacta taaggatgcg cttggcagtc
      841 atgaagctgt agtccgttga ttccgttaag cgagcattac aacgatcgtt tgtgacagtc
      901 ccgtgacagg gactatttga gcgcgcgttc ggcgctggtc aaggccactt tgtcgcgtaa
      961 atacaccggg cagagctcgc ccggatcgag accgtctccc aaacccagcc gcagcgcacc
     1021 aagcctcgcg aactcgcgcg cgctcggcag ggcgtctgat gcttctgcgg cgacggtgag
     1081 accgggcagg cgcgcgagca gcgggtaggc ggcaaagccg cggccgactc cctgataact
     1141 gccatccgtt ggcaacgcga cgctctcggg tggccctacg cgcgcggcac cggcggcgag
     1201 cagaccttca gtcgggtcgg ccgcgaagca gccccaatag acttcgccca tccgggcatc
     1261 gagacaggcg agcgcccgcg tcgctccggc ccgaaatagc ggcatcgcca aggcctccag
     1321 ggtcgtgacc gccgccaccg gcaagcccga accgaaagca agcccctggg caaccgccac
     1381 gccgatccgc actccggtga atgcaccggg acccacgctc gcggcgatgc catcgagcgc
     1441 ggcgagcgtc agcccggcct cggccagcag tgcatcgacc atgtcgagaa tctgtgacgc
     1501 ttcgcagcgg ccgccatgca tcatccggtg tgtcaattca tcgccattga gaagcgccac
     1561 cgagcacgcc tccgttgcgg catccaatgc caacacgcgc acgggtcagg tcacttgcga
     1621 ggcaagcgct ctcgccggac gcagcttcgc cagtcgctcg accagaaacc gggcaccggc
     1681 ggcgccgtcg gtgatgaccg gactcggtgg caatgcctgc aagaacacac gcccatagcc
     1741 cttggtcctg agccgcggat caccgaccac gatcacgccg aaatcatcga agtcgcgaat
     1801 caagcgccct accccttgtt tcaatgccaa caccgcctgc ggcagctggt actcgaaaaa
     1861 cgggttgccg ccacgccgcc gaatgccttc gagtcgtgcc ttcagcagcg ggtcgtcggg
     1921 cgaggcgaac ggcaacttgt cgatcgcgac gatcgagagc gcctcgcctt tgacgtccac
     1981 gccttcccaa aaacttcccg tggccagcag cacggcattc cccagttcgc gaaagcgatt
     2041 cagcaacgct tcgcgcggcg cttcgccctg aaccaggact ggaaatgtcg ggtttgggaa
     2101 gcgcgcgtgc agcgcccgga ccccctccgc cagacctcga tagctggtgt agagcaggaa
     2161 ggcgcgtccg ccgctcgcct cgagcagcgg cgcgcaagcg tcgatgaagc gcgccgcaaa
     2221 cgccggatcc tgcggctgcg gcatgtgcgg cggcagaaag atacgggcct gatcggcata
     2281 gtcgaacggg ctgtctatgt gcaccgtgcg cgcctcaggc atgccgaccc gcgccgcaaa
     2341 atgcgaaaaa tcttcgccga tggccagcgt cgcggacgtg aaaatccagg cgcagggccg
     2401 tgcctcgacg tattgccgca gccgttgcgc aatttcgaag ggcgtgagtt gcaacagcat
     2461 tccactcgcg ttggcgtcga cccagcgcaa gccctcatcg tccggcagct cgcgcagcgc
     2521 cgcgagcgag ttcgccatga cggcggcgcg gcgcgcgcag ttggcgagcc cggcacctgc
     2581 cccaagaccc tcgagtcgag tcgcgaggtc gctgatggcg gtctcgagtt ccggcaatgc
     2641 gtccaaaaaa gactccggca agcttgccca ttcgatgcgc ccgggcgcgc cttgcaggct
     2701 cgagcgcagc gcctcgatgc aggtgtcgac gccggcggcg cactcggtga ttgccggcgc
     2761 atacacgctg cccgccgtca gttccgccgc gatatcgcgc agcaacagct gcacctggcg
     2821 caccgaccag agttgaccga aaaactgcgc cgcgatgtcc ggaatctgat gcgcctcatc
     2881 caaaatgacg gcctccgcgc cgggcaacag atcgccgaag ccttcgtctt tcaacgcaag
     2941 atcggccagc aacaggtggt ggttgacgac cacgatatct gccgcctgtg ccgtgcgtcg
     3001 cgcctcgacc acatgac
//
LOCUS       DASWZT010000159        10065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_68888, whole genome shotgun sequence.
ACCESSION   DASWZT010000159 DASWZT010000000
VERSION     DASWZT010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10065
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68888"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..1812)
                     /locus_tag="VGE92_04320"
     CDS             complement(181..1812)
                     /locus_tag="VGE92_04320"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEY1313075.1"
                     /translation="MLQPGLMMDQPLLISGILQHAVAQFGQTPIVSRETHGPVFRYDY
                     ATLGRRVSKLAHALAHLGVKPGNAVGSLAWNNHRHLEAYYAVSGSGMVIHTINPRLHP
                     EQLIYIINHAEDRALLFDATFAPLIKGIAAECPRVETWICLSEAAQLPDGDGIPHLRC
                     YEELIDASSEHFAWPEFDERTAAALCYTSGTTGNPKGALYSHRSIVLNALTICCPGLL
                     SVCSAETILPIVPMFHINGWCIPYAALIGGAKLVLPGPRLDGAGLYELMEREGVTVSA
                     GVPTVWLGLTQHLEMHDLRLSTLRRIISGGSALPRPLIATLCERHGLDVRHGWGMTET
                     TAVATISALDCEQMTWAPAYRHSIIAKQGKSVFGVELKVVDEQGRSLPRDGHSQGELM
                     VRGQWIINGYYKAQSSPLVDGWFPTGDIATIDAHGVMQIRDRVKDLIKTGGEWISSID
                     LENVAIGHPAVAAAAVIGVLHPKWQERPLLFVVRKPGRTLERDEILEFLSRQVARLCV
                     PDEVVFLDSLPVGGTGKVQKALLRENYGGVFTQRE"
     gene            1909..3372
                     /locus_tag="VGE92_04325"
     CDS             1909..3372
                     /locus_tag="VGE92_04325"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HEY1313076.1"
                     /translation="MNAKLPSRLGLLAAAMLPLYAAASSHREAPAIAGEPRVDGTDFY
                     MFRSYEPGRSAYVTFIANYIPLQDAYGGPNYFNLDPAADYEIRIDNNGGGIGDLRFVF
                     KFTNTYKDLAVEAGGDKIPVPLINIGPIDAAGANLNLIQSYTVGVSRNFGPPEPLGNA
                     TLGGSKFYKPTDNIGKKSIPDYAEYAAHFIYEVNIPGCAKPGRVFVGQRKDGFVVNLG
                     EVFDLVNLNPAGPRDGEPNTLTDKNVTSIAIEVPISCVTQGKDPVIGAWTTASLPRRG
                     GDGWQQVSRLGMPLVNELVIGLPDKDKFNSSPPLDDAQFLKYVTNPSLPVLLNALFGS
                     AATVPGTPRNDLVAAFLTGVKGINQPLKVTPGEMLRLNTSIAPTAPASQNDLGVLGGD
                     LAGFPNGRRPYDDVVDITLRVAEGALCGAIGNCGKDTSDPNHGIPYTDGARAAGPDAA
                     NSHVTGAINAADTYADSFPYLMTPLPGSPNGVNGEPK"
     gene            complement(3414..3884)
                     /locus_tag="VGE92_04330"
     CDS             complement(3414..3884)
                     /locus_tag="VGE92_04330"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1313077.1"
                     /translation="MTLKSVSIRSATPQDVPALATLVQRYWEFESLGGFARARIEMLL
                     GDLLSKPDRGACWLAEAQGQVCGYLLAVFVFSLEHGGLMAEIDELFVSDELRSVGAGS
                     LLLARADRDLAERGLTRLQLQLGVHNHRARHFYERHGFARRAGYELLDKALEAT"
     gene            complement(3889..6093)
                     /locus_tag="VGE92_04335"
     CDS             complement(3889..6093)
                     /locus_tag="VGE92_04335"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEY1313078.1"
                     /translation="MISHTILKCVGRGSVAALFSSAVFAAAVPSMPGNLDAARLKAAD
                     KEPQNWFTAGRDQNGSYFSPLANIGAANVKQLGFAWAYDLGYPMRGQEATPIVIDGVM
                     YTSGTWGYVYAVDAASGKELWRYDPKPNYFIGRHPCCDLVNRGVAVWKGKVYVASVDG
                     RLHALSAATGKKIWEADTITDHKLPYSSTGAPQIAGNVVIIGNSGADMGHTAVRGYVA
                     AYDVETGAFKWRFYTVPPAVGKPYENPELAQADKTWDHGRKPEFNGGGTAWDGFAYDP
                     DLNLVYFGTANAAPYDLRQLGPAKLDSLYTASIVALDATSGRMAWYYQTTPHDSWDYD
                     SVQKMILAELPLGGAPRSVIMQASKNGFFYVLDRKTGELLCAKNYTYVNWASHIDMKT
                     GRPVVVSAAADWYSSPKNVYPSWSGGHTWNPMSLSAQTHLVYIPVIDQPAVWIDLQHN
                     GGRVKFIEGFFTVNGVYPDDTYDAAALKSLFGPLPDLHALRATRKVNLVRELLRAWDP
                     VAQKIVWEHETSSGIRGYDGGTMSTAGNLVFQGRGSGELWVYAADTGKVLKVIKTGSH
                     IMASPMTYSVGGEQFVAVQAGYGGTAITVGPIPPSSAALKYQNTNRIIAFKIGGGAVP
                     TPPARIDAPFEKPPAATATQAQIEAGEVKFTEQCTRCHQLGPSSTPDLRKLNAGLHAA
                     FKDIVLKGVLAPAGMERFDDILSETDVDNIHAYLIDQSWLAYRQQQSAAARP"
     gene            6263..7096
                     /locus_tag="VGE92_04340"
     CDS             6263..7096
                     /locus_tag="VGE92_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEY1313079.1"
                     /translation="MAALSLATFSLAASADDSVNPNKSDYSLFDPTPDDEMRSFSTDR
                     PPKANSPYTVDAGHFQYETDLAVFGYGNTNGTTTHDWTVIDPTLKVGLTNTIDAELQI
                     TPYESLTTKTAAGHVSVSGIGDTFGRLKINVLGDDQGTVSIAVLPYLKFPTARSGLGN
                     GKVEGGIILPVSFNAPGGFTIIVMPEGDFLKNDVGNAYHAAVDFLINVSHPLDRRWTI
                     YTEIFTSQYFESQDPPVYTLDEALTYALTPNLQIDLGGNFGLNKVTPRKQLYAGLAQR
                     F"
     gene            complement(7125..8462)
                     /locus_tag="VGE92_04345"
     CDS             complement(7125..8462)
                     /locus_tag="VGE92_04345"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HEY1313080.1"
                     /translation="MKERLTLLIRRAIYNMRGGFLVRPLIIALVLGCVGAVLSWLEEV
                     VPTLSSWVPVVLFPSHADPQVAQVILAGIAASIMTVVSIVFAILLMTLTMASMQFSPR
                     IMLSFTRDRVTQWTLGIFLGTFLYCIAALPAAHSVPTPFSPVATVLGAVVLALACVCW
                     LLFFIQHIALAISVNRIVDRIAAETEAVIDEMMPWPRQEVSLPKQELPQGHIFELTIA
                     SERSGYIRFIDVDRLLAVAKFHRISVRVLRRVGHFVPAHVALLAVSKSERLPPEGKAA
                     LLGAFDIGASRTLQQDVEFGVLQIVDIALRAISPAVNDPSTAISCVDQLSRILIRFIS
                     REPPASMLYDLPGVPRVILAWIDFDGLLDAAFEQTRLYSKTDVAVSLRMLRALGDISS
                     TTAVPAFRRTLAQRGRRIAAGCAARLGDEDLGKMDERLRTLERLLAQVETAGA"
     gene            8674..9555
                     /locus_tag="VGE92_04350"
     CDS             8674..9555
                     /locus_tag="VGE92_04350"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEY1313081.1"
                     /translation="MNRSWPIWRSMLFVPAHVEKFVAAAHTRGADAYILDLEDSVPPA
                     EKATARDSLARSAARVAQSGAAVLVRVNGDTALLGDDLQASVVPGVRALMLPKIGSGD
                     DVRAIDAQIGEIEGQRGLPPGHTLLIAQIEHVSTLPRLDEIAASSTRLMGMILGSEDF
                     SVSAGMEPTLEALYAPNQQMLFACRRAGILPFGFPGSIGVFRDVEQLRLLIRRAREMG
                     FVGSYAIHPNQVTVMNELFLPAAEEVEHARELLAGWDTALAQGRGAFEFRGRMVDPPV
                     VARAQEVLRRFTAAARS"
     gene            9759..10065
                     /locus_tag="VGE92_04355"
                     /pseudo
     CDS             9759..10065
                     /locus_tag="VGE92_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008365064.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
BASE COUNT         1949 a         3139 c         3094 g         1883 t
ORIGIN      
        1 ccctccttgg cggcccgcgc caccgcctgc gcgttgcgtt tgaagatgct gccgatggtc
       61 ttggcatcag aggtcgcgcc cggcgcatcc gccggcggct cgtccgccgc tgcctgcgcg
      121 gccatcaaca ccaacgccgc gcccagcact gcgaatcggt gaatcgtctt agtcatgatt
      181 tcactccctc tgggtgaaca cgccgccgta attctcgcgc agcaacgcct tttgcacttt
      241 gccagttccg cccaccggca gcgagtcgag aaaaacgacc tcgtccggga cacataggcg
      301 ggccacttgc cgcgacagga attcaagaat ctcgtcgcgc tcgagcgtgc gccccggctt
      361 gcgcaccacg aacagtaacg gtcgctcctg ccacttcggg tgcagcacgc cgatgacggc
      421 tgctgctgcc accgccggat gtccgatggc gacgttctcg agatcgatcg agctgatcca
      481 ttcgccgccg gtcttgatca ggtctttgac gcggtctctg atttgcatca cgccatgcgc
      541 atcgatggtg gcgatatcgc cggtcggaaa ccatccgtcg acgagcggcg aggactgtgc
      601 cttgtagtag ccgttgatga tccattgtcc gcgcaccatc aattcgcctt gcgaatgccc
      661 atcgcggggc aagctgcggc cctgttcatc gacgaccttg agctccacgc cgaacaccga
      721 tttcccctgc ttcgcgatga tcgaatgccg atacgcgggc gcccacgtca tttgctcgca
      781 atccaaggcg ctgatggtcg ccaccgcggt ggtttcggtc attccccaac catggcgtac
      841 atcgagacca tggcgttcgc acaaggtggc gatcagcggc cgtggcagcg cggaaccgcc
      901 ggagatgatg cggcgcagcg tcgacagccg caaatcgtgc atctcgagat gctgtgtcag
      961 cccgagccac acggtcggca cgccggcact gaccgtgacg ccttcccttt ccatcaattc
     1021 gtagaggcct gcgccgtcca agcgggggcc gggcagaacc aacttggcgc cgccgatcag
     1081 cgccgcatac gggatacacc agccattgat atgaaacatg gggacgatcg gcaaaatcgt
     1141 ctcggcggaa caaaccgaca acagcccggg gcagcaaatc gtgagcgcgt tcagcacgat
     1201 cgatcggtgg gagtacagcg cgcccttggg atttccggtg gtaccggagg tgtagcacag
     1261 cgcggcggcg gtgcgttcgt cgaattcggg ccatgcgaaa tgctcgctcg aggcgtcgat
     1321 cagctcttcg tagcaccgca agtggggaat gccatcgccg tcgggcagct gcgccgcctc
     1381 gcttaggcaa atccatgtct cgactcgcgg gcactcggcc gcgatgcctt tgatcaacgg
     1441 cgcgaaagtg gcgtcgaaca gcagcgctcg atcctcggcg tggttgatga tgtagatcaa
     1501 ctgttccgga tgcaggcgag gattgatggt gtggatcacc atgccgctgc ccgagaccgc
     1561 gtaataggct tcgaggtgtc gatggttgtt ccacgcgagg gagccgactg cgttcccggg
     1621 tttcacgccc aagtgcgcca gcgcatgggc gagtttcgag acgcggcgcc caagggtggc
     1681 atagtcatag cggaacacgg gaccatgcgt ctcgcgcgac acgatgggcg tctgtccgaa
     1741 ctgcgccacg gcatgctgca atatgccgga gatcagcaac ggctgatcca tcatgagtcc
     1801 aggctgcagc atcgtttgcc cccatgcatc caattcgcga cggctctcgt atccccttgg
     1861 cctgtcaaat gcgctggttt gctgcactca acaaccaagg ggtttccaat gaacgctaaa
     1921 ttgccgagtc gcttaggctt gctggccgcg gcgatgcttc cattatatgc cgccgcgtcg
     1981 agccaccgag aggcgccggc cattgccgga gagccgcggg tcgatggcac ggacttttac
     2041 atgtttcgta gctacgaacc ggggcgtagc gcgtacgtaa ccttcatcgc caactacatt
     2101 ccgctgcaag acgcctatgg cgggccgaat tatttcaatc tcgaccccgc cgccgattat
     2161 gagatccgca tcgacaacaa cggcggcggc atcggcgatc tgcgcttcgt gttcaaattt
     2221 accaatactt ataaggactt ggcggtggaa gcgggcggcg acaagatccc cgtgccgttg
     2281 atcaatattg ggccgatcga cgcggcgggc gcgaatttga acctgatcca gagctacacg
     2341 gtcggagtgt cgcgcaattt tgggccgccc gagccgctcg gcaatgccac cctcggcggc
     2401 agcaagttct acaaacccac cgataatatc ggcaagaaat cgatccccga ctacgccgag
     2461 tacgcggcgc acttcatcta tgaagtgaac attcccggtt gcgccaagcc gggccgggta
     2521 ttcgtcggcc agcgcaagga cggcttcgtc gtcaatttgg gcgaagtatt cgacttggtg
     2581 aatttgaacc cagccggacc gcgggacggt gagccgaaca cgctgaccga caagaatgtc
     2641 acctcgatcg cgatcgaggt gccgatctcc tgcgtgacgc agggcaagga tccggtcatc
     2701 ggggcgtgga ccacggcgag cctgccgcgc cggggcgggg atgggtggca gcaagtctcc
     2761 cgactcggca tgccgctggt caacgaactc gtgatcggat tgcccgacaa ggacaaattc
     2821 aactcgagcc cgccgctcga tgacgcccag ttcttgaaat atgtcaccaa cccgtctctg
     2881 ccggtgttgc tcaatgcttt gtttggcagc gcggcgaccg tgccgggcac accgcgcaac
     2941 gatttggtgg cggccttcct gaccggcgtc aaggggatca accaaccgct caaggtgacg
     3001 cccggagaaa tgctgcggtt gaatacttcg atcgcgccga ctgcgccggc ctcacagaac
     3061 gacctgggcg tgttgggcgg cgacttggcc ggtttcccga acggccggcg cccgtacgac
     3121 gacgtcgtcg acatcacctt gcgggtggcg gagggcgcgc tgtgcggagc gatcggaaat
     3181 tgcggcaagg acacgagcga cccgaatcac ggcattccgt acaccgatgg cgcgcgcgcg
     3241 gcaggccccg acgccgccaa ttcgcacgtc accggagcga tcaatgcggc ggatacgtac
     3301 gcggatagtt tcccgtactt gatgaccccg cttcccggat cgccgaatgg cgtgaacgga
     3361 gaacccaagt aggggcacca ggcgccacgg cgtgaccgcc gtggcgccga ttttcacgtg
     3421 gcctcgagcg ccttgtcgag caactcgtag cccgcccgcc gcgcgaaccc gtgccgttca
     3481 tagaagtgcc gcgcacggtg attgtgaacg ccaagctgta actgcaaacg cgtcagcccg
     3541 cgctccgcga ggtcgcggtc ggcgcgcgcc agcagcagcg agcccgctcc taccgagcgc
     3601 aactcgtcgc tcacgaacaa ttcgtcgatc tccgccatca aaccgccgtg ttcgaggctg
     3661 aacacgaaaa ccgccaacaa atacccgcac acctgtccct gggcctccgc caaccaacag
     3721 gcgccgcggt ccggcttcga caaaagatcg ccgagcagca tctcgatgcg cgctcgagca
     3781 aaaccgccca aggactcgaa ttcccagtag cgctgcacga gagttgcaag cgccgggacg
     3841 tcctgaggcg tggccgagcg gatagatacg gatttcagcg tcatcgactc aagggcgcgc
     3901 cgccgcgctt tgctgctgtc gataggcaag ccaactctgg tcgatcaagt aggcatggat
     3961 gttgtcgacg tccgtctcgc tcaaaatgtc gtcgaagcgc tccatgcccg ccggcgccag
     4021 cacgcctttc aacacgatgt ccttgaaggc tgcatgcagc ccggcattga gcttgcgcag
     4081 atcaggcgtg ctgctcgggc ccagttgatg acagcgtgtg cactgttcgg tgaatttgac
     4141 ttcgccggcc tcgatttgcg cctgagtggc ggtcgccgcc ggcggcttct caaacggtgc
     4201 gtcgatgcgc gccggcggtg tcggtaccgc gccaccgccg atcttgaagg caatgatccg
     4261 attggtgttc tgatacttga gagccgcact gctcggggga atgggcccga ccgtgattgc
     4321 cgtcccgccg tagcccgctt gcaccgcgac gaactgctcg ccgccgacgg aataggtcat
     4381 cggcgacgcc atgatgtggc tgccggtctt gatcaccttg agcaccttgc cggtgtcggc
     4441 ggcgtagacc cagagttcgc cgctgccgcg tccctggaac accaagttcc ccgcggtcga
     4501 catcgtgccg ccgtcgtagc cgcggattcc cgacgaggtc tcgtgttccc agacaatctt
     4561 ttgagccacc gggtcccagg cgcgcagcag ctcgcggacc aaattgacct tgcgcgttgc
     4621 gcgcagcgcg tgcaaatccg gcagcggtcc aaacaggctc ttgagagcgg ccgcatcgta
     4681 agtgtcatcg ggatagaccc cgttcaccgt aaagaaccct tcgatgaact taacccgccc
     4741 gccgttgtgc tgcaaatcga tccacaccgc gggttgatcg atgaccggga tgtagaccag
     4801 atgcgtctgt gcactcaagg acatcgggtt ccaggtatgg ccacccgacc aagagggata
     4861 aacgttcttc ggcgagctat accaatcggc cgccgcggaa accaccaccg ggcggccggt
     4921 tttcatgtcg atgtgggatg cccagttgac gtaggtatag ttctttgcgc agaggagctc
     4981 gccggtcttg cggtcgagaa cgtagaagaa gccgttcttc gatgcctgca tgatgaccga
     5041 ccgcggggcg ccgccgagcg gcaactcggc aagaatcatt ttctgaaccg agtcgtaatc
     5101 ccagctgtcg tgcggcgtgg tttggtagta ccaggccatg cgcccgctgg tcgcgtcgag
     5161 cgccacgatg gatgcggtat acaggctgtc gagcttcgca ggtccgagct gccgcagatc
     5221 gtacggtgcc gcattggcgg tgccgaagta gaccaggttc aagtcaggat cgtaggcgaa
     5281 cccgtcccat gccgttccgc cgccgttgaa ttcaggcttg cgaccgtgat cccaagtctt
     5341 gtcggcttgc gccagctccg gattctcata gggctttccg accgccggag gaaccgtata
     5401 gaagcgccat ttgaatgcgc ccgtctccac atcgtaagcc gcgacatagc cgcgcaccgc
     5461 ggtatgaccc atgtcggcgc cgctgttgcc gatgatgacg acattgccgg cgatttgcgg
     5521 cgcgccggtg ctcgagtagg ggagcttgtg atcggtgatc gtgtccgctt cccagatttt
     5581 cttgccggtc gcggcgctca gcgcatgcag ccgtccatcc acggaggcga catagacctt
     5641 gcctttccaa accgcgacgc cgcggttgac cagatcgcag cacgggtgtc tgccgatgaa
     5701 gtagttgggc ttcgggtcgt agcgccagag ttccttgccg ctggccgcat cgaccgcata
     5761 cacatagccc caggtgcccg aggtatacat gacaccgtcg atgacaatcg gggttgcttc
     5821 ctggccgcgc atcggatagc cgagatcgta agcccaagcg aagccgagct gcttgacgtt
     5881 ggccgcgccg atgttcgcca acggggaaaa atagctgcca ttctggtcgc ggccggccgt
     5941 gaaccagttt tgcggctcct tatccgcagc cttgagccgc gcagcgtcga ggttgccggg
     6001 catgctcgga acggccgcgg caaacacggc gctcgagaac agagccgcga cgctgccccg
     6061 gccgacgcac ttcagaatcg tgtggctgat caagtcttat tctcctgccg gatcgggtca
     6121 aagcttagcg aacatcccgg gcgaacggta ccatgccgca aacactccga ggtccggttg
     6181 gatgcgtcgc tatggtaagc gaatgaccct acgtctaatg tcggatcagc tgtcgaatcg
     6241 cctcggatgc cgagcagcag ttttggctgc gctgtcgctg gcgacattct cacttgctgc
     6301 atcggcggat gattcggtaa atccgaataa gagcgattac agcctgtttg atccaacccc
     6361 cgacgatgag atgcggtcct ttagcacgga tcggcccccc aaagccaata gcccctacac
     6421 agtcgatgcc gggcattttc aatacgagac tgatcttgcc gtctttggct acggaaatac
     6481 caacgggacg acaacacatg attggaccgt gatcgacccg acactcaagg tcgggctgac
     6541 caataccatc gacgcggaac tgcagattac gccgtacgaa tcgctgacaa ccaaaaccgc
     6601 ggccggtcac gtgagcgttt ctggtatcgg cgataccttc ggacgattga agatcaatgt
     6661 gctcggcgat gatcaaggca ctgttagtat cgccgtgttg ccctacctca agtttccgac
     6721 ggcacggtcg gggcttggca acggcaaagt ggaagggggg atcattctgc ctgtaagctt
     6781 caatgcgccc ggcggcttta ccatcatcgt catgccggaa ggggattttc tgaagaacga
     6841 cgtggggaat gcatatcatg ccgcggttga ttttttgatc aacgtgagcc acccgctcga
     6901 caggcgatgg actatttaca ccgaaatttt tacgtcccag tacttcgaat cgcaagaccc
     6961 ccccgtctat accctggatg aggcactcac ttacgcactg accccaaatc tgcagatcga
     7021 tttaggcggc aacttcgggt tgaataaagt gacgcctcgc aagcagctct atgcgggcct
     7081 tgcacagaga ttctagatct caaattctga tgatctgccg gcgatcacgc tcctgccgtc
     7141 tcgacctgcg ccaggagacg ctcgagcgtg cgcagccgct cgtccatctt cccgagatcc
     7201 tcgtctccca gccgggcggc gcagcccgcg gcgattcggc ggccgcgctg cgcgagcgta
     7261 cgccgaaacg ccggcactgc cgtggtcgag gagatgtcgc ccagcgcgcg cagcatgcgc
     7321 aggcttaccg caacgtcggt tttcgagtac agacgcgtct gttcgaaggc cgcatcgagc
     7381 agcccgtcga aatcgatcca ggccaggatc acgcggggca cgccgggcag atcgtatagc
     7441 atcgaggctg gaggttcgcg cgagatgaac cggatcagaa tgcggctcaa ttgatcgaca
     7501 cagctgatcg ccgtactcgg gtcgttcaca gcgggggata tggctcgcaa agcgatgtcc
     7561 acgatctgca gcacgccgaa ctccacatcc tgctgcagcg tgcgcgaggc gccgatatcg
     7621 aacgccccga gcagcgccgc ctttccttcc ggcgggagcc gctccgactt ggacaccgcc
     7681 aacaaggcga catgcgcagg gacgaagtgg ccgacccggc gcagcacgcg cacgctgatg
     7741 cgatggaatt tcgccacagc gagcagccgg tcgacgtcga tgaaccgaat atacccggag
     7801 cgctcgctcg ctatggtcaa ttcgaagata tgtccttgcg gcagctcctg ctttgggagg
     7861 gaaacttctt ggcgcggcca gggcatcatt tcatcgatca ccgcctccgt ctcggcggca
     7921 atcctgtcga cgatccgatt gacgctgatg gcaagcgcga tgtgctgaat gaagaagagc
     7981 agccagcata cgcacgcgag cgcaagcact acggcaccga ggaccgtcgc gacgggagag
     8041 aagggtgtgg gcaccgagtg ggcggccggc aaggcggcta tgcagtagag gaacgttccg
     8101 aggaagatgc ccagcgtcca ctgcgtcacc cgatctcgcg tgaagctcag catgatccga
     8161 ggagagaact gcatcgaggc catggtgagc gtcatcagca ggatcgcgaa gacgatggac
     8221 accaccgtca tgatggaggc ggcaatgccc gcgaggatga cctgggcgac ctggggatcg
     8281 gcgtgggaag ggaagagcac caccggtacc cagctgctca gggtcgggac cacttcctcc
     8341 agccaggaca gcacggctcc gacgcagccg agcacgagcg cgatgatgag cgggcgcacc
     8401 aagaacccgc cgcgcatatt gtagattgcg cgtcgtatca gtagagtcaa ccgctctttc
     8461 atcgttctcc ggtcctgaac ctggcgattg gccagccgac tgcgggaact cggcaggttg
     8521 ccgcggtacc ccgaccgtga attctacacg ggcggcgcat caagccagcg acgaatcgag
     8581 gtaggatacg acgcgaattc tttgaagcgc tcatggaaag aatatgaatt cgggaacgac
     8641 gaactccggc atgcgcgcta cggcgaagct cgcgtgaatc gctcgtggcc aatctggcgc
     8701 tcgatgttgt tcgtgccggc gcacgtggag aagttcgtcg cggccgcgca cacgcgcggc
     8761 gccgatgcct acattctcga tctcgaagac agcgtgccgc cggctgagaa ggccacggcg
     8821 cgcgactcgc tcgcgcgctc cgccgctcgc gtcgcgcagt cgggtgccgc ggtgctggtt
     8881 cgcgtcaacg gcgatacggc gctgctcggc gatgatttgc aggcgagcgt tgtccccggc
     8941 gttcgcgcgt tgatgttgcc caaaatcggc agcggcgatg acgtgcgcgc catcgatgcg
     9001 cagattggcg agatcgaagg acagcgcggt ctgccgccgg gccatacact actgattgca
     9061 caaatcgaac acgtgagcac actgccgcgc ttggatgaga tcgcggccag ttcaacccgg
     9121 ctgatgggca tgatcctggg ttcagaggat ttttcggtct cggcgggcat ggagccgacg
     9181 ctcgaggcgt tgtacgcacc gaaccagcag atgctgttcg cgtgccgccg cgccggcatc
     9241 ctgccgttcg gttttcctgg atcgatcggt gtgtttcgcg atgtggagca gctgcgcctt
     9301 ctcatccgca gggcccggga gatgggattc gtcggttcct atgcgataca cccaaaccaa
     9361 gtcacggtca tgaacgagct atttctgccg gccgccgaag aagttgagca tgcgagggaa
     9421 ctgcttgccg gctgggacac ggcgctcgcc caggggcgcg gagcctttga attccgcggc
     9481 cggatggtcg atccgccggt cgtggcgcgt gcccaggaag tgttgcgccg cttcaccgcg
     9541 gcggcccgct cgtgaatgcc gttgcgcaat acgcaaatca tttgcgccac agttaaattt
     9601 tgtggttata tccgatcatg gtacccctcc gccaaaccgg cgtcgcgccc gacaaatggc
     9661 gctaagtccg tatgccccat cgattacttc gtttcggttt ctcgcgcagg caccccgaac
     9721 cgcgaatgtt tacgcggcac gactcgttgc gggccggcta tgacgtggtc atcatcggtg
     9781 cgggggggca tggactggcg gccgcgtact atctggctcg cgatcacggc atcagcaatg
     9841 tagcggtcct ggagaagggc tatctcggcg gcggcaatac gggccgcaat accaccatca
     9901 tccgttcgaa ctacctcacc cccgacgggg tgaagttcta cgacgagagt gtccggctgt
     9961 ggcaggacct ggccaccgac ttcgacttga atctcttcta ctcgacccgc ggccattaca
    10021 ccctggcaca caccgacgcc gcgatgcgca ccatgcgctg gcggg
//
LOCUS       DASWZT010000160         3661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_1180, whole genome shotgun sequence.
ACCESSION   DASWZT010000160 DASWZT010000000
VERSION     DASWZT010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /gene="tauD"
                     /locus_tag="VGE92_04360"
     CDS             <1..824
                     /gene="tauD"
                     /locus_tag="VGE92_04360"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HEY1313082.1"
                     /translation="VSRLTPALGAIVEGVKLADELPQESVDRLNELLVEHQVLFFREQ
                     PITPQQQCRFAARFGQLHVHPIYPVLPDLPQILLLDTHGDFLPDNDNWHTDVTFSQTP
                     PMAGILAAKQLPSAGGDTLWSSNLAAYEALSAPMKRLLEGLTAEHSVLKSFPAARWGA
                     DPASKERYERAVAKHPPVTHPVVRTHPVSGRKSLFVNDGFTTHIHELNPRESDALLGF
                     LFAHAARPEFTLRWRWRVDDLAFWDNRITHHYAVADYLPERRVMHRATVIGDRPF"
     gene            complement(821..2248)
                     /locus_tag="VGE92_04365"
     CDS             complement(821..2248)
                     /locus_tag="VGE92_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY1313083.1"
                     /translation="MNTDPRGSSGLHHALRQRHMTMIALGGVIGAGLFVGSGVVIKAA
                     GPAAVVSFAITGLLVVLVMRMLGEMAVALPAVGSFYEYARLAWSDRPQLGELAGFLAG
                     WMYWYFWVIVVALEAVAGAGLVRFWLPNVPPWFASLTLLLSLTLTNLVSVKSFGEFEF
                     WFASIKVAAITAFLLIASVYVLGLWPGTTASIANLTGHGGFAPNGLLPILTGAVAATG
                     FYFGAEIVSIAAAEAAEPAKAVARATNSVVSRALFFYVGSILLVVCLVPWNSAQIMTP
                     YVSALAAMRIPAAAHIMNAVVLTAVLSALNSGLYASSRMLFALTRRGDAPAELAKLGA
                     NGVPARAILLGTVFGYGAVIMSYVSPDAVFAFLVNSYGTVAIFVYVLIAVSELRLRAR
                     LEREDPARLKIRMWAYPYLTYLAIAGMLGIVLAMGFIPEQRTPLIFGLVSLGILLLAF
                     GLRARFGSKPMIATLALEARGYEEL"
     gene            2338..2838
                     /locus_tag="VGE92_04370"
     CDS             2338..2838
                     /locus_tag="VGE92_04370"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HEY1313084.1"
                     /translation="MPRIMLSAILCCAGLGGVSQAGDVPDVKPGLWKTTTTTGDPNVP
                     AQSGTMCTSTALLQTLFDQRLKDPARPCKQTSVVHSGTTITEQTECKFDGTVKRNKVV
                     TVVSGDTEVRTEIHEEGKNTVTVSDSKWLSACPAGMQLGDFVGADGMKFNILHPENAR
                     APAKAP"
     gene            2947..3177
                     /locus_tag="VGE92_04375"
     CDS             2947..3177
                     /locus_tag="VGE92_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313085.1"
                     /translation="MNKFQAQIKQSPPITLLHTLTCPNLARCTPTDTLRVVRARNVAE
                     AVLDLIETYMADDVRRTVCVCGCAKGGKPTSD"
     gene            3337..>3661
                     /locus_tag="VGE92_04380"
     CDS             3337..>3661
                     /locus_tag="VGE92_04380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY1313086.1"
                     /translation="MTTNITDFLVIVPTYRRPEFLAAALRSALSQSGVSKQIIVVDDC
                     PDGSAGPIVASLDPAIIYLKNPHPSSGWPARVRNYGFDTSLQMGIRARFVHFLDDDDT
                     VPDGHY"
BASE COUNT          786 a         1169 c         1096 g          610 t
ORIGIN      
        1 cagtttcccg tctgacgccc gcactcggtg ccatcgtcga gggtgtcaag cttgccgatg
       61 aactgccgca ggagtcggtc gatcgcctca acgagctgtt ggtcgagcac caggttctgt
      121 tctttcgcga acagccgatc actccccaac agcaatgccg gttcgcggca cgcttcggcc
      181 aattgcacgt tcatccgatc tatcccgtgt tgcccgactt gccgcagatc ctattactcg
      241 atacgcacgg tgattttcta cccgacaatg acaactggca cacggatgtg acgttcagtc
      301 agaccccgcc gatggccggc atcctcgccg ccaagcaact gccgagcgcc ggcggcgaca
      361 ctctctggtc gagcaatttg gccgcgtatg aggcgttgtc ggcgccgatg aagcgcctat
      421 tggagggact taccgccgaa cactccgtgc tcaagtcctt tcccgccgca cgctggggag
      481 ccgatcccgc atccaaggag cgctacgagc gtgcggtcgc gaagcatccc ccggtgaccc
      541 atcccgtggt gcgcacgcac ccggtctccg ggcgcaagag cctgttcgtc aacgatggct
      601 tcacgaccca cattcacgag ctgaatccgc gggagagcga cgcgttgctg ggtttcctat
      661 ttgcgcacgc ggcgcgcccg gaatttaccc tgcgctggcg ctggcgcgtc gatgacctgg
      721 cgttctggga caaccggatc acgcatcatt acgccgtcgc ggattacctg cccgaacggc
      781 gcgtcatgca ccgcgcgacg gtcattggcg accgcccttt ctagagttct tcgtatcccc
      841 gcgcttcgag cgcgagcgtc gcgatcatcg gcttgctacc gaaacgggcg cgcaggccga
      901 aagcaagcag cagaataccg aggctgacga ggccgaagat gagcggcgtg cgctgctcgg
      961 gaatgaagcc catcgcgagc acaatgccga gcatgccggc gatcgccaaa taggtcagat
     1021 aaggatacgc ccacatgcgg attttcaggc gcgccggatc ctcgcgctcc agacgcgcgc
     1081 gcaggcgcag ctcggaaact gcgatcagaa cgtacacgaa tatcgccacg gtcccgtacg
     1141 agttcacgag aaaggcaaac accgcgtccg gcgagacata cgacatgatc accgcgccgt
     1201 agccaaacac ggtgccgagc agaatcgcgc gcgctggcac gccgttggcg ccgagtttcg
     1261 ccaattccgc cggcgcatcg ccgcgccggg tgagcgcgaa cagcatgcgc gaggacgcgt
     1321 acagtcccga gttcagggcg gacagcacag cggtgaggac cacggcattc atgatatgcg
     1381 ccgccgcggg gatgcgcatg gcagcgagcg cactgacgta cggagtcatg atttgggcgg
     1441 aattccacgg caccaggcac acgaccagta ggatggagcc cacgtagaag aacaaggcgc
     1501 ggctcacgac cgagttggtt gcccgggcga cggctttggc gggctcggcg gcctcggctg
     1561 ccgcgatgct gacgatttcc gcgccgaaat aaaagccggt ggccgcgacc gcccccgtca
     1621 atatgggaag caggccgttc ggcgcgaagc cgccgtgtcc cgtcagattg gcgatgctcg
     1681 cggtcgtacc cggccatagg ccgagcacgt acacgctcgc gatcagcaag aatgccgtga
     1741 tcgccgcgac tttgatcgag gcaaaccaga attcgaattc accgaatgac ttcaccgaga
     1801 ccagattggt caacgtcagc gacaataaca acgtcaaact ggcgaaccag ggcggcacgt
     1861 tcggcagcca gaagcgcacc agcccggcac ccgccaccgc ttcgagcgcc accacgatga
     1921 cccagaaata ccaatacatc cagccggcga ggaagccggc gagttcgccg agttgcggcc
     1981 gatcgctcca tgcgagccgc gcgtactcgt aaaatgagcc gaccgcgggc agagcgacgg
     2041 ccatttcgcc caacatccgc atcaccagca ccaccaagag accggtgatc gcgaacgaaa
     2101 cgacggccgc cggtcccgcc gccttgatga ccacaccgct gcccacaaac agacccgcac
     2161 cgatgacgcc gcccaaggcg atcatcgtca tgtgtctttg tcgcaaggca tggtgcaggc
     2221 cgctcgagcc gcgaggatca gtgttcacac cggcactcta ctggatttcg ccgtgaatgg
     2281 cgtccgtgtc aaagcaaccg cataatggcg ccactgaacc gactgcctgg agcgtgcatg
     2341 cctcgaatca tgttatcggc aattctgtgt tgcgcagggc tcggcggcgt ttcgcaagcc
     2401 ggcgacgtgc cggacgtcaa gccggggttg tggaaaacga ccacgaccac cggcgatccg
     2461 aacgtccccg cgcaaagcgg caccatgtgc accagcacgg cgctgttaca gactctcttc
     2521 gatcagcggc tcaaggaccc cgcgcggccc tgcaagcaga ccagcgtggt gcactccggc
     2581 accacgatca ccgagcagac ggaatgcaaa ttcgacggca cggtgaaaag aaataaagtg
     2641 gtcaccgtcg tcagcggcga tacggaagtt cgaacggaga ttcatgagga aggcaagaac
     2701 acggtcaccg tcagcgacag caagtggttg agcgcgtgtc ccgcgggaat gcagctcggc
     2761 gatttcgtcg gcgccgatgg catgaagttc aatattctcc accccgagaa tgccagggcg
     2821 ccggcgaaag ctccctgaat ccagggttaa catatcctca aaaatcgcga ttggttcacg
     2881 gaatggcacg tgacataagc agtagcgtcg accgcccagt gatcggaaac atccggggta
     2941 acgcacgtga acaaattcca ggcgcaaatc aagcaatcac cgccgattac gctgctccac
     3001 acgctgactt gcccgaacct cgctcgatgt accccgactg acacattgcg cgtggttcgc
     3061 gcgcgcaacg tcgccgaggc ggtgctcgat ttgatcgaga catatatggc cgacgatgtg
     3121 cggcgcacgg tctgcgtgtg cggctgcgcc aagggcggca aaccgacgtc cgattgacgc
     3181 gcgccgccgc cgtccgccag aacgtgcgcc agtcctcagg cggcaaaata atatggcgaa
     3241 tcgacgcgga attttccgta agaagagtga cccatgtcgc gccgcgcgcg aaatgccgtg
     3301 acaagatgtt acgacagggc agtaagcagc ttggtaatga ctacaaacat cacggatttc
     3361 ttggtcatcg ttcccacgta tcgacgaccc gaatttttgg ccgcggcgct gcgcagcgca
     3421 ttgagtcaat caggcgtgag caagcagatc atcgtcgtcg atgactgccc ggacggatcg
     3481 gcggggccga tcgtagcgag cctcgatcct gcgatcatct acctgaagaa cccgcacccc
     3541 tcgagcggct ggccggcgag agtccgcaat tacggcttcg atacatcgct gcagatgggt
     3601 attcgggccc gtttcgtgca ttttctggat gacgatgata ccgtaccgga cggccattac
     3661 g
//
LOCUS       DASWZT010000161         7140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_44738, whole genome shotgun sequence.
ACCESSION   DASWZT010000161 DASWZT010000000
VERSION     DASWZT010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7140
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44738"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VGE92_04385"
     CDS             <1..376
                     /locus_tag="VGE92_04385"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY1313087.1"
                     /translation="SYEIYAAAPESVDLDAFVDRDSRKYTPYTLPAGTHMYASFGHLA
                     GYSSAYYTYMWDKVIAEDFFEQFDAKDLLAGGTPLRYRRTVLEPGGSESANDLVKNFL
                     GRPQSVAALQRWMSAEFAPPPR"
     gene            476..1162
                     /locus_tag="VGE92_04390"
     CDS             476..1162
                     /locus_tag="VGE92_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEY1313088.1"
                     /translation="MANFPTSNAFPGVDSRAAFITRTYTHLVGGILGFILVELGLFES
                     GLAAQIAQFMLGFNWFLILGAFMLTGWLASRTAQTSSSIGMQYFAFGAYVVAEALIFV
                     PLLYIADAKAPGAIDSATLVTALGAGGLMLVAHRTRKDFSFLRALLMWGGVLALVAMI
                     GGAIFGFQLGTWFSVAMIGFAGAAVLYDTSNIIHNYPEDRYVAAAMQLFASIALMFWY
                     VLRLVMGSRN"
     gene            complement(1212..2483)
                     /gene="serS"
                     /locus_tag="VGE92_04395"
     CDS             complement(1212..2483)
                     /gene="serS"
                     /locus_tag="VGE92_04395"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY1313089.1"
                     /translation="MLDPKLLRSDLSGVAAALKRRGFVLDVAAFAAVEERRKAVQIEA
                     DRLRAERNANAKAVGQAKSKGQDTAALLAAGESLGNDLQSIDRQLEAIQAEVSDLQLG
                     LPNILHTSVPDGDNEAANVEVRRWGSPRQFDFTPRDHVALGERIGMDFEAAGRISGAR
                     FVVLHATLARLHRALAQFMLDLHVDKHGYREAYVPYLVHAAALLGTGQLPKFEADLFA
                     MRGEPGFYLIPTAEVPLTNLVREQILAPEKLPLKLVAHSPCFRSEAGAAGKDTRGMIR
                     QHQFDKVELVHIVAPEHSYAALEELTSNAEAVLQALQLPYRVLSLCAGDTGPSSAKTY
                     DLEVWLPSQQRYREISSCSNCESFQARRLQARWRNPVSGKPELVHTLNGSGVAVGRAL
                     VAVLENYQQEDGSVAVPGVLAPYMGGIAELK"
     gene            complement(2526..3839)
                     /locus_tag="VGE92_04400"
     CDS             complement(2526..3839)
                     /locus_tag="VGE92_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016486902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEY1313090.1"
                     /translation="MTGGDGTYRPLADRLRPQSLDEYVGQSHLLGPGAALRRALESGR
                     PHSMILWGPPGTGKTTLARLVASGANAQFIALSAVMAGIKDIRAVVEQAQALRGSRDT
                     VLFLDEVHRFNKSQQDTFLPYVEDGTLIFVGATTENPSFEVNNALLSRARVYVLKALD
                     AADLGKLLDRALSEADRGLGKLNLQIDSAARELLLAAADGDARRMLNLLETAADLSTP
                     EATGRRLDVDTTRAVIGSTYVRFDKGGENFYDQISALHKSVRGSDPDAALYWLCRMLA
                     GGCDPLYIARRVLRMASEDIGNADPRALTLGLEACAVYERLGSPEGELAIAQAVVFMA
                     CAAKSNAVYTAYLAAAKDAAGLGSLEVPLHLRNAPTRLMKELGYGKGYRYAHDEPGGY
                     AAGERYFPDSMPDRRYYVPAARGLEIKIGEALNARRERDAAAGKS"
     gene            complement(3836..4492)
                     /gene="lolA"
                     /locus_tag="VGE92_04405"
     CDS             complement(3836..4492)
                     /gene="lolA"
                     /locus_tag="VGE92_04405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEY1313091.1"
                     /translation="MTPDRRSRCSSLVAGVSLMILAAVLRAAPASGPAGDVDKYLTGL
                     SSWTADFTQTIDDGHGKVMRSAAGKLFLQKPGKFRWDYSQPSEQVILADGKQIWFYDK
                     DLAQANVRDMDSSLASTPASLLSGSSSVSTEFNVTALPPNGGLQWFQLIPKRADTDFQ
                     LVRIGFDKGDLRSMFLADKLNQVTQLTFSNSKRNVTFAPDLFSFVPPPGVDVIGRGGK
                     "
     gene            complement(4494..6830)
                     /locus_tag="VGE92_04410"
     CDS             complement(4494..6830)
                     /locus_tag="VGE92_04410"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEY1313092.1"
                     /translation="MADDVVQKKPRRKSGLSAAVERSLREGTLWVLAALALLLLLALL
                     SYHAHDPGFADTGEPGQAVGNWIGPVGAWLAGFFLFLFGRPAYLFPLMLVYAGWLVHK
                     EQPLPDARSRVNSLLRAAGFALTLVTSCGLATLHWSAAGLPNSAGGVLGELAGRGSSR
                     ALSFLGATLLLLGLWLAGVALSLSVSWFDAMDQLGAAVLKALEWTRARFEQRRELAGG
                     LKRKQARQEVVREEQKKVANRPPPRIESPAPVPQKSDRVERERQVPLFDAPKSSELPA
                     LSLLDEPGPREQSYSDEALEAMSRLVELKLRDFGVEAEVVAVFPGPVITRFELRPAPG
                     VKVSQISNLSKDLARALSAISVRVVEIIPGKSVVGLEIPNEKREIVTLGEIIKSKPYD
                     DVSSPLVLALGKDIGGVPIVADLARMPHLLIAGTTGSGKSVGINAMVLSLLYKATPEH
                     VRMIMIDPKMLELSVYEGIPHLLSPVVTDMKQAANALRWCVAEMERRYQLMAMLGVRN
                     IGGFNRKVKDAADSGKPIKDPIRMQKIAQGVPGVVAEDVPDLTSLPFIVVIIDELADL
                     MMIVGKKVEELIARLAQKARASGIHLILATQRPSVDVITGLIKANIPTRIAFQVSAKV
                     DSRTILDQSGAESLLGHGDMLYLPPGTSIPTRVHGAFVSDQEVHRVVGGLKSASPPIY
                     IEEILEGPRNPIPGFPSDDEEAEGEAADAEADPLYDEAVRIVTETRKASISGVQRRLK
                     IGYNRAARLVEAMEAAGLVGPLQSNGAREIYGAAPPEE"
BASE COUNT         1322 a         2347 c         2121 g         1350 t
ORIGIN      
        1 gtcttatgag atctacgccg ccgcccccga gagcgtggat ctggacgcgt tcgtcgatcg
       61 cgacagccgg aaatacacgc cctacacgct gccggcgggc acgcatatgt acgcctcttt
      121 cgggcacctt gccgggtatt catcggctta ctacacttac atgtgggaca aggtcatcgc
      181 cgaagacttc ttcgagcaat tcgacgccaa agatctgctc gccggcggca caccgctgcg
      241 ctataggcgt acggtcttgg agccgggagg ctcggagtcg gccaacgatt tggtcaagaa
      301 cttcctcggc cgcccgcagt ccgtggcagc cctgcagcgc tggatgagcg cagaattcgc
      361 tccgccgccg cgttgaaggc ggccgtttca cccccaatat caggcggtcg ggccaaatcg
      421 gcaccccgct cgcgggggtg ctaaaatagc ccatcggtta actggagaca tgcacatggc
      481 caattttcca accagcaacg cttttccggg tgtggacagc cgcgctgcat tcatcacccg
      541 cacctacacc cacctggttg gcggaatcct cggcttcatc ctggtcgagc tgggactgtt
      601 cgaatccggc ttggcggcgc aaatcgcgca gttcatgttg ggcttcaact ggttcctgat
      661 actcggtgcc ttcatgttga ccgggtggct ggcatcgcgc accgcccaaa ccagctcctc
      721 gatcggcatg cagtacttcg cgttcggggc ttacgtcgtc gcagaagcgc tgattttcgt
      781 gccgctgctg tacatcgcgg acgcaaaggc tcccggcgcg atcgatagcg ccaccttggt
      841 gacggcgctc ggtgccggcg gtctcatgct ggttgcgcac cgcacccgca aggacttctc
      901 tttcctgcgg gctctgctca tgtggggcgg cgttcttgcg ctggtcgcaa tgatcggcgg
      961 cgcaatcttc ggcttccagc tcggcacctg gttctcggtg gccatgatcg gcttcgccgg
     1021 cgccgcggtg ctctacgata cctcgaacat cattcacaac tacccggaag atcgctacgt
     1081 ggcggcggca atgcagttgt tcgcctccat cgcactgatg ttctggtacg tgttgcgcct
     1141 ggtcatgggc agccgcaact agtcgcgaaa ccctttcggt gaagcgcagc gcgggccttt
     1201 acggcccgcg ctcatttcag ttccgcgatt ccccccatgt agggcgcaag cacgcccggc
     1261 accgcgacac tgccatcctc ctgctgatag ttctcgagca ccgcgaccag cgcccgccct
     1321 accgcgaccc ccgagccgtt cagcgtatgc accagttcgg gcttgcccga tacgggattg
     1381 cgccaccggg cctgcaaacg tcgcgcctgg aacgattcgc aattgctgca ggatgaaatc
     1441 tcccggtagc gctgttgcga gggcagccaa acctcgagat cgtacgtttt tgcgctgctc
     1501 ggtccggtgt cgccggcgca cagcgacaaa acgcgatacg gcaactgcaa tgcctggagc
     1561 acagcttcgg cattcgatgt cagctcctcg agcgccgcgt aggagtgttc cggggcgacg
     1621 atgtgcacca attccacttt atcgaattga tgctggcgaa tcatgccgcg cgtgtccttg
     1681 ccggccgccc ccgcctcgga gcgaaaacac ggtgagtgcg ccaccagctt gagcggcagc
     1741 ttttccggcg cgaggatctg ctcacgaacc aagtttgtca gcggtacctc ggcagtcgga
     1801 atcaagtaga agccgggctc gccgcgcatg gcgaacaaat ccgcctcgaa tttgggcaat
     1861 tgtccggtcc cgagcagcgc cgcggcgtgc accagatacg gcacatacgc ttcgcgatac
     1921 ccatgcttgt cgacatgcag gtccaacatg aattgcgcga gggcgcggtg cagtctcgcg
     1981 agcgttgcat gcagcacgac gaagcgtgct ccggagattc taccggcagc ctcgaaatcc
     2041 atgccgatgc gctcgccgag tgccacgtga tcgcgcggcg taaagtcgaa ctgccgcggg
     2101 cttccccagc gccgtacttc gacgttggcc gcctcattgt ctccgtcggg cacgctcgta
     2161 tgcagtatgt tgggcagacc caactgcaag tccgacacct cggcttggat tgcttcgagc
     2221 tgcctgtcga tcgattgcaa gtcgttaccc aaggactcgc ccgcggcgag caacgccgcg
     2281 gtgtcctgac ctttggactt ggcctggccc acggccttgg cattcgcgtt gcgctcggcg
     2341 cgcagccggt cggcttcgat ttgcacggcc ttacgccgct cttccaccgc agcgaaggcg
     2401 gccacgtcga gcacgaagcc gcgccgtttg agcgcggcgg cgacgccgct caaatcgctg
     2461 cgcagcagct ttggatcgag cacggtgatt cctcaggttg acagcgagag cttattgtag
     2521 gcgaatcaag acttgccggc ggccgcgtcg cgctcgcgac gggcgttcaa ggcttcgccg
     2581 attttgattt ccaagccgcg cgccgcgggt acgtaataac gccgatccgg catcgagtcg
     2641 ggaaaataac gctcacccgc cgcatagccg ccgggttcat catgggcata tcgatacccc
     2701 ttgccgtagc ccagttcctt catcaaacgg gtgggcgcat tgcgcaagtg cagcggcact
     2761 tcgagcgaac ccaagcccgc ggcgtccttg gccgccgcca ggtaggccgt atagacggca
     2821 ttgctcttgg ccgcgcacgc catgaatacg actgcttggg caatggctaa ttcgccctcc
     2881 ggactgccca agcgctcgta caccgcgcaa gcttccaatc ccagggtgag cgcacgcgga
     2941 tccgcgttgc cgatatcctc gctcgccatg cgcaacacgc gccgcgcgat gtacaaagga
     3001 tcacagccgc cggccaacat gcggcacagc cagtacagag cggcatccgg atcgctgccg
     3061 cggaccgatt tgtgcaaggc tgaaatctgg tcgtagaaat tctcgccgcc tttgtcgaac
     3121 cgcacatagg tgctgccgat cacggcgcgc gtggtgtcga cgtcgagccg gcggccggtt
     3181 gcctcgggcg tactcaaatc ggcggcggtc tccagtagat tcagcatgcg acgcgcatcg
     3241 ccgtcggccg cggcaagcag cagctcgcgc gcagcggaat cgatttgcag attgagcttg
     3301 cccaaccccc ggtcggcctc gctcagcgcg cggtcgagca gtttgccaag atcggcggcg
     3361 tcgagcgcct tcaacacata cacccgcgcc cgtgacaaca acgcattgtt gacctcgaag
     3421 gacggattct cggtggtcgc gccgacgaag atcagagtgc cgtcctcgac atagggcagg
     3481 aaggtatcct gctgcgattt gttgaagcga tgcacctcgt cgagaaacag caccgtatcg
     3541 cggctgccgc gcaacgcctg cgcctgttcg accaccgcgc ggatatcctt gatccccgcc
     3601 atcaccgccg acagcgcgat gaactgcgca ttcgcgccgc tcgccaccag tcgcgcgagc
     3661 gtggttttgc cggttcccgg cggtccccac aaaatcatcg aatgcggccg gcccgattcc
     3721 aaggcgcgcc gcagcgccgc tccgggaccc aacaaatgag attgccctac atattcgtcg
     3781 agagactgcg ggcgcagccg atcagccaac gggcgatagg tgccgtcgcc gcccgtcact
     3841 tgccgccgcg cccgatcaca tcgacgcccg gcggcggcac gaacgaaaac aggtccggcg
     3901 caaatgtcac attgcgcttg gagttcgaga aggtcagctg ggtgacttga ttcagcttgt
     3961 cggcgaggaa catggagcgc aaatcaccct tgtcgaagcc gatgcgtacc agttgaaaat
     4021 ccgtatccgc tcgtttgggt atgagctgga accactgtaa cccgccgttg ggcggcaaag
     4081 cggtcacatt gaattcggtg ctcaccgaac ttgaccccga cagcaggctc gccggggtgc
     4141 tggcaagact cgagtccata tcgcgcacat tggcctgcgc cagatccttg tcgtagaacc
     4201 agatttgctt gccgtcggcc aagatgacct gctcggaggg ctgggaatag tcccagcgaa
     4261 acttgcccgg cttttgcagg aacagcttgc cggcggcgct gcgcatcact ttgccgtggc
     4321 catcgtcgat cgtctgggta aaatccgcgg tccagctcga gagcccggtc aaatacttgt
     4381 cgacatcgcc ggccggccca gacgcgggcg ccgctcgcag cactgccgcc aggatcatca
     4441 ggctcactcc ggcaaccagg ctgctgcagc gcgatcttcg atcaggtgtc acgtcattcc
     4501 tcaggcggcg cagccccgta gatctcgcgg gcgccgttcg attgcaaggg gcctactaaa
     4561 cccgcggctt ccatggcctc gaccagtcgc gccgcccggt tgtagccgat cttcaggcgg
     4621 cgctgcacgc ccgagatgga tgccttgcgc gtctcggtga cgatgcgcac ggcctcgtcg
     4681 tacagaggat cggcttcggc atcggcagcc tcaccttctg cttcctcgtc atcggatgga
     4741 aatccgggaa tcggattgcg gggtccttcg agaatttctt cgatgtagat tggcggcgat
     4801 gcggacttga gccctccgac cacccgatgg acctcttggt cggagacgaa cgcgccgtgc
     4861 acccgagtcg gaatcgaggt acccggcggc agatacagca tatcgccatg gccgagcagc
     4921 gactcggcgc cgctctgatc gagaatcgta cgcgagtcca ctttggccga gacctggaat
     4981 gcaatccgag tggggatgtt cgccttgatc agccccgtaa tcacatcgac cgacgggcgt
     5041 tgcgtcgcca agatgagatg gatcccggac gcgcgcgcct tctgcgccag ccgcgcgatc
     5101 aactcctcga ccttcttgcc gacgatcatc ataagatcgg ccaattcgtc gatgatgacg
     5161 acgatgaacg gcaatgaggt caggtccggt acgtcttcgg cgaccacccc cggcacccct
     5221 tgtgcaatct tttgcatgcg gatcggatcc ttgatgggct taccagaatc cgcggcgtcc
     5281 ttgaccttgc ggttgaagcc gccgatgttg cgcacgccga gcatcgccat cagctggtag
     5341 cgccgctcca tctctgcgac gcaccagcgc agggcgtttg ccgcctgttt catatccgtt
     5401 acgaccggcg acagtagatg tggaatgccc tcgtacaccg acagctccag catcttcggg
     5461 tcgatcatga tcattcgcac gtgttccggc gtcgccttgt acaacaggct cagcaccatg
     5521 gcgttgatcc ctaccgactt gcccgagccc gtggtaccgg cgatcagcaa gtgcggcatg
     5581 cgcgcaaggt ccgcgacgat cggcacgccg ccgatatcct tgccaagcgc caagaccagc
     5641 ggcgaactga cgtcatcata gggtttggat ttgatgattt cgcccaacgt cacgatctcg
     5701 cgcttctcgt tgggtatttc cagtcccacg acggacttgc cgggaatgat ttccaccacc
     5761 cgaacgctga tcgcggaaag cgcgcgcgca aggtccttcg atagattgct gatctggctc
     5821 actttgacgc cgggcgcggg gcgcaattcg aagcgcgtga tcaccggacc cggaaacacc
     5881 gccaccacct cggcctcgac cccgaagtcg cgcagcttga gttccaccaa gcgcgacatc
     5941 gcctcgagcg cctcgtcgga gtacgactgc tcgcgtggcc ccggctcatc gagcagggac
     6001 agcgccggca gttcgctcga ttttggcgca tcgaacagcg gcacctgccg ctcgcgctcg
     6061 acgcggtcgc tcttctgcgg taccggagca ggcgactcga tgcgcggcgg cgggcgattt
     6121 gccacctttt tctgctcttc gcgcacgacc tcttgccgcg cctgcttgcg tttgaggccg
     6181 ccggccagtt ctcgtcgctg ttcgaatcgt gcgcgggtcc actcgagcgc cttcaagacc
     6241 gcggcgccga gttggtccat ggcgtcaaac catgacacgc tcaatgagag cgcgacgccc
     6301 gccagccaca atcccaggag cagcagtgtc gcgccgagaa aactcagggc gcgcgagctg
     6361 ccgcgacccg caagttcgcc aagcacgccg cccgcgctgt tcggcagccc cgccgcgctc
     6421 caatgcagcg ttgcaaggcc gcagctcgtg accaaggtca atgcaaatcc cgccgcgcgc
     6481 agcaggctgt tgacgcgcga acgcgcgtcg ggcagaggct gctccttgtg gaccagccaa
     6541 cccgcgtaca ccaacatcag cggaaataga tacgccgggc ggccgaacag aaatagaaag
     6601 aatccagcca gccaggcgcc caccggaccg atccaattgc cgaccgcttg cccgggttcg
     6661 ccggtatcgg caaatccggg atcgtgggcg tgataggaca ggagcgcgag cagcaacaac
     6721 aatgcaagcg ctgccagtac ccagagcgtg ccctcgcgca aagaccgctc gacagcggcg
     6781 gagagcccgc tcttgcgccg cggcttcttc tgtacgacat cgtcggccaa ttctagacct
     6841 ttaatgatat ttacttctta agtgattgaa taatatgtga tagtaactca gtctcacaga
     6901 gggcgccaac accgaccgat gcttagagta ggcgtcctgc gcagccttga ttgaactggc
     6961 tggcgcccgc agatttactt accatgagcc ggtaatgatc tagaagagag gcaaattata
     7021 catgagcgcc cagaggcaca gtccactcct gatcctgggg tcgggtcccg ccggctactc
     7081 cgcggccgtg tatgcggcgc gtgccaatcg ccacccactg ctcataaccg gtctcgagca
//
LOCUS       DASWZT010000162         3040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_1209, whole genome shotgun sequence.
ACCESSION   DASWZT010000162 DASWZT010000000
VERSION     DASWZT010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1480
                     /locus_tag="VGE92_04415"
     CDS             44..1480
                     /locus_tag="VGE92_04415"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF015406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEY1313093.1"
                     /translation="MPITTLVWFRQDLRLADNPALAAALEFAAPIIPVFIFAPAEEGT
                     WAPGAASRWWLHQSLSQLDAQLRKAGSRLCLRVAADSLTELLRLCRECGATRVLWNRR
                     YEPTVIARDRRIKTELQAAGLETGSFNAALLHEPWSVMTGSGGPFQVFTPFWRHCTAL
                     PDPVEPSPAPARIAAPAAWPESRALGDLSLLPTLNWTQGLHAAWSPGNGAEVLRQFLD
                     DSFDEYPSVRDRPDLPGTSRLSPYLHFGEIGPRQVWHAVRRFALNRGQHTSWRQSHFL
                     TELGWREFAHHLLFHFPQTPEQPLREKFARFPWQSSALRLRAWQRGATGYPLIDAGMR
                     QLWRTGWMHNRVRMISASFLVKDLLQPWTDGARWFWDTLVDADLASNTLGWQWVAGCG
                     ADAAPYFRIFNPITQATRFDPQGTYVREWVPELARLTSEWVHQPWAAPAEILADAGVR
                     LGSSYPHRLVEHATARAAALAALATLKK"
     gene            1528..2331
                     /locus_tag="VGE92_04420"
     CDS             1528..2331
                     /locus_tag="VGE92_04420"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY1313094.1"
                     /translation="MHARVGLNEISGREESTRPVRALNRGLDVLCELNRRGRAAINTL
                     AGALRLPRTTTYRILETLRSAGYVDRDPHDDCYRPTIMVRSLSDGFDDEAMVAHIAKP
                     HLAALGAQIVWPVAVATPSGGAMMIRETTDRESPLAIEHYAAGVRVPMLGSAAGRAYL
                     AFCSAAQRDALLELLARSSLPDDRLARSRSEVERLLNETRTQGFGMAHRARRVSEETS
                     LAIPVRAKDRILAAVTVRYAASAVPLRTAVEQFLPKLREFAQRIETEFV"
     gene            complement(2337..>3040)
                     /locus_tag="VGE92_04425"
     CDS             complement(2337..>3040)
                     /locus_tag="VGE92_04425"
                     /inference="COORDINATES: protein motif:HMM:NF017835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AcvB/VirJ family lysyl-phosphatidylglycerol
                     hydrolase"
                     /protein_id="HEY1313095.1"
                     /translation="AVPTKLAYGRFGEVAVYAADAQARDVVLFLSGDGGWNQGVISMA
                     QRLSLAGAVVAGIDIRHYLAQLEKSTDKCVSPAADLANLSHFLQSSLRLKHDLRPTLV
                     GYSSGATLVYATLAEAAQGSFKGALSLGFCPDLDLKKPLCTGSGVAATPRRDAKGVLK
                     GVDFLPAKQLSGKWISLQGEIDQVCPAPATRKFIATVPDGEIVMLPKVGHGYSVERNW
                     VPQYEAAYRRITAGE"
BASE COUNT          540 a         1007 c          928 g          565 t
ORIGIN      
        1 gtccttaatt ttccgcccta aattggtcgc aaattgtagc tgaatgccta taacgacact
       61 ggtttggttt cggcaggacc tgcgcctcgc cgacaaccct gccctggcgg ccgcgcttga
      121 atttgccgcc cccatcattc cggtgttcat attcgcgccg gcggaggaag ggacttgggc
      181 tccgggcgca gcgagccgtt ggtggttgca ccagagcttg tcgcaactcg atgcgcagtt
      241 gcgcaaagcc ggctcgcgac tctgcctgcg cgtcgccgcc gactcgttga cggagcttct
      301 gcgcctgtgt cgagaatgcg gcgcgacgcg cgtactgtgg aatcgccgct acgagcctac
      361 ggtgattgcg cgcgatcgtc gtataaagac cgagttacag gccgccggtc tcgaaaccgg
      421 cagcttcaac gccgcgctgc tgcacgaacc ctggagcgtc atgaccggca gcggcggacc
      481 cttccaagtg ttcacgccct tctggcgcca ttgcacggcg cttccggacc cggttgagcc
      541 ctcccccgca ccggcgcgca tcgccgcccc tgccgcctgg ccggagtcgc gcgcgcttgg
      601 cgatctgtcg ctgttgccaa cgctcaactg gacgcaagga ctgcacgccg cttggtcccc
      661 cggcaatggc gcagaggtgc tgcggcaatt cctggatgat tccttcgacg aatatcctag
      721 cgttcgtgat cggccggact tgcccggcac ctcgcgcttg tcgccgtatc tgcacttcgg
      781 agaaatcggc ccgcgacagg tctggcacgc ggtgcgccgc ttcgcgctga accgcggcca
      841 acatacgagc tggcgccaat cgcattttct cacggagctc ggctggcgcg aattcgcgca
      901 ccatctgctg tttcattttc cgcaaacacc cgagcaaccg ttgcgcgaga aattcgcgcg
      961 ctttccatgg caatcctcgg cgctgcgtct gcgcgcatgg cagcggggag cgaccggcta
     1021 tccgctcatc gacgcaggca tgcgccaatt atggcggacg ggatggatgc acaatcgcgt
     1081 gcgcatgatc agcgcgtcgt tcctggtcaa ggatctgctg caaccctgga ccgacggagc
     1141 gcgctggttc tgggacacat tggtcgatgc cgaccttgcc agcaatacgc tcggctggca
     1201 gtgggtggcg ggttgcggcg ctgacgcggc tccctatttc agaatattta acccgatcac
     1261 gcaggcaacc agattcgatc cgcagggtac ttatgtacga gagtgggttc cggagctcgc
     1321 gcgcctgacg agtgagtggg tgcaccaacc atgggctgcg cctgccgaga tcttggcgga
     1381 tgccggcgta cggctcggat cgagctatcc gcaccggctt gtcgaacacg ctacggcacg
     1441 agccgcggcg ttggccgcat tggcgactct caagaaatag cgtatgggtt tgtttcgtag
     1501 catagaatac tggtaaggaa gacgataatg cacgcacggg tcggtctcaa tgaaatttcc
     1561 ggccgtgaag aatcgacccg cccggtccgc gcattaaatc gcggcctcga tgtgctttgc
     1621 gaattgaatc gccgcggacg cgccgcgatc aacaccctgg ctggcgcgct gcgcctgccg
     1681 cgaaccacca cctatcgcat cttggagacg ctgcgcagcg ccggctacgt ggaccgcgat
     1741 ccgcatgatg attgctaccg gccgaccatc atggtgcgct ccctgtcgga cggctttgac
     1801 gatgaggcca tggtggccca tatcgccaaa ccgcatctcg cggcgttggg cgcgcaaatc
     1861 gtttggccgg tggcggtggc aacgccgtcg ggcggcgcca tgatgatccg cgaaaccacc
     1921 gaccgcgaaa gtccgctcgc catcgaacac tacgctgccg gcgtgcgcgt accgatgttg
     1981 ggatccgcgg cgggacgtgc ctacctcgcg ttctgctccg ccgcgcaacg cgatgcactg
     2041 ctcgagcttc tggcgcgctc gtcgctgccg gatgatcgcc tggcgcgcag ccgcagcgaa
     2101 gtcgagcgat tgctcaacga gacccgcaca caagggttcg gcatggcgca tcgcgcgcgg
     2161 cgcgtatccg aggaaaccag cctcgcaatt ccagtgcgtg ccaaggaccg aatcctcgcc
     2221 gccgtcacgg tacgttacgc cgccagcgcc gtgccgctgc gcactgcggt cgaacaattc
     2281 ctgccgaagc tgcgcgagtt tgcgcaacgg atcgaaaccg aattcgtctg actcgcctac
     2341 tcgccggcgg tgatgcgccg gtacgccgct tcgtactgcg gcacccagtt tcgttccacc
     2401 gaatagccgt gtccgacttt cggcaacatg acgatctcgc cgtcaggtac ggtcgcgatg
     2461 aatttgcgtg tggcgggtgc cggacacacc tgatcgattt ccccttgcag cgaaatccac
     2521 ttgccggaga gttgcttggc gggcagaaaa tcgacgccct tcagcacacc cttcgcatcg
     2581 cggcgcggcg tggccgcgac gccggatccc gtgcacagcg gcttcttcaa atccagatcc
     2641 ggacaaaatc cgagggacag agcgcccttg aatgagcctt gcgccgcttc tgccagcgtc
     2701 gcatacacca gggttgcgcc cgacgaatag cccaccagcg tcggccgcaa gtcgtgcttc
     2761 aggcgcaacg aggactgcag aaaatgactc aaattcgcca ggtccgccgc cggcgatacg
     2821 cacttgtccg tggacttctc caactgcgcg agatagtggc ggatgtcgat gcccgccacg
     2881 accgcgcccg ctaagctaag ccgctgcgcc atggaaatca cgccctgatt ccagccgccg
     2941 tcgccggaca gaaacaacac cacatcgcgc gcttgcgcgt cggccgcgta caccgcgacc
     3001 tcgccgaatc ggccgtaggc aagcttcgtc ggaacagccg
//
LOCUS       DASWZT010000163         2459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_83377, whole genome shotgun sequence.
ACCESSION   DASWZT010000163 DASWZT010000000
VERSION     DASWZT010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83377"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VGE92_04430"
     CDS             <1..915
                     /locus_tag="VGE92_04430"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEY1313096.1"
                     /translation="YPDRPIRLIVSIAAGSVTDVIMRTAAVELTPLLGGQPLVIENRG
                     GASGIVAAQACAQAAPDGYTLCVIYHSTMSYNPLLFDHLPYDPDTDLVPVTRLFFLNE
                     GLFVSSELGVNSVAELKKMAQTKSDQLNYATLGDGSFPDLFLRWMNNQWGTKIVGVPY
                     KGGGPAAQALAGNQVQLTRFGIGNFLGLVKAGKVKAIAVSADKRSPLLPDVPTLAEAG
                     LGGYPGQGWWGLAAPKGTPPALVDKINAVFVKVFSDPKFMAYLDQQAVVPAPTTPAGF
                     VAFLKEDRKAAEALIKIANSKREAYKPE"
     gene            1045..>2459
                     /locus_tag="VGE92_04435"
     CDS             1045..>2459
                     /locus_tag="VGE92_04435"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY1313097.1"
                     /translation="MDGEVKPQAMGEEPSRSLLAFFGRRRVSGTIDPAGAPRARARWM
                     LSASQFVVVLAAIALGAALLCGAIGFGLANRSDERLWAEQRASLRNATAEFRNLFDRS
                     DAVDPRFIRMVEQSVGVNGLKFETDPPPSEREMQPVMDAQGRIAGFLTWDRAHPMFQA
                     MQRLMPFIAVVAFVLVGFAGFSLRQLRRARLQLAASEQQARRAAEEDALTGLPNRRKM
                     LVLLENAMAARVGQKLLTFAMFELSGLHEIDEEIGMIRREEFLAEAVMGTKDYLPANA
                     TLGRMGTTEFAVICDGEADARTMLRSMVRAITGLQCGDTDARIGAHAGYAEAPRDATG
                     SDELVRRARVALRAASEKGSGIVTAFEHAIDAVSKDQQFIRRELPRAVAAEALELHYQ
                     PIVASDGSGLTGVEALLRWTHPERGAIGPAVFIPVAEQMGLIDRLGAFVLRRALHEAK
                     RWPDLYISVNLSPLQVRDPGIV"
BASE COUNT          429 a          766 c          815 g          449 t
ORIGIN      
        1 tatccggacc gtccgatccg gctgatcgtc tcgatcgccg ccggcagcgt caccgatgtc
       61 atcatgcgaa cggccgcggt cgagctcacg ccgctgttgg gcggccagcc gctggtgatc
      121 gagaaccgtg gtggcgccag cggcatagtc gcggcgcagg cctgcgccca agccgcaccc
      181 gatggctata cgctgtgcgt gatctaccac agcacgatgt cctacaatcc gctgctgttc
      241 gatcatttgc cctatgaccc cgataccgat cttgtgccgg tgacgcggct gttctttctg
      301 aatgaaggct tgtttgtatc gtcggagttg ggcgtgaact cggtcgccga gctcaagaaa
      361 atggcgcaga ccaaatcgga ccagctcaac tacgccacgc tcggcgacgg ctcatttccg
      421 gacctgttcc tgcgctggat gaacaaccag tggggcacaa aaatcgtcgg cgtgccgtac
      481 aaaggcggtg gtccggcggc gcaggcgctc gccggcaatc aggtgcaatt gacacgcttc
      541 ggcatcggaa attttcttgg actggtgaaa gccggcaagg tgaaggcaat cgcggtatcg
      601 gccgacaagc gctcgccgct gctgcctgac gtcccgacat tggccgaggc cggccttggc
      661 ggttatcccg gccagggctg gtggggccta gcggcgccga aaggaacgcc gcccgcgctg
      721 gtcgacaaga tcaatgcggt cttcgtgaag gtgttcagcg atccgaaatt catggcgtat
      781 ctggaccagc aggcggtggt gccggcaccg accacaccgg cgggcttcgt cgcgtttctg
      841 aaagaggatc gcaaggccgc ggaggcgctg atcaaaatcg cgaacagcaa gcgcgaggcg
      901 tataagccgg aatagctttc gcaggcccgc ttttggtgag caagcggtcc ggttacgttt
      961 cattcacctt gtttgctgac ccgccattaa cgctaaaatc ctagcgttct ccctggattg
     1021 ccgcgcagtt gcggggagat ttaattggac ggcgaggtca aaccgcaagc gatgggcgaa
     1081 gagcccagcc gctcgctgct ggcgtttttc ggacgccgcc gcgtaagcgg aacgatcgat
     1141 cctgccggcg cgccgcgcgc gcgcgcccga tggatgctgt cggcctcgca atttgtcgtc
     1201 gtgctggcgg cgatcgcctt gggcgcggcc ttgctgtgcg gcgccatcgg ctttggcctg
     1261 gcaaaccgga gcgacgagcg gctgtgggcc gagcaacgcg cctcgctgcg caatgcgacg
     1321 gccgagttcc gcaatctgtt cgacaggtcc gatgcggtcg atccacgatt catccgcatg
     1381 gtcgagcaga gcgtcggcgt caacggcctc aaattcgaaa ccgatccgcc gccaagcgag
     1441 cgcgagatgc agccggtaat ggatgcgcag ggccgcatcg ccggctttct cacctgggac
     1501 agagcgcatc cgatgtttca ggcgatgcag cggctgatgc cgttcatcgc agtggttgcc
     1561 ttcgtgctgg ttggcttcgc cggattttca ctgcggcagt tgcgccgcgc acggctccaa
     1621 ttggcggcaa gcgagcagca ggcgcgccgc gccgccgagg aagacgcgct gaccggcttg
     1681 ccgaaccgcc gcaagatgct ggtgctgctc gagaacgcca tggcggcgcg cgtcgggcaa
     1741 aagctgctga cgtttgcgat gttcgaattg tccggcctgc acgaaatcga cgaagaaatc
     1801 ggcatgatcc gccgcgagga atttctcgcc gaagcggtga tgggtaccaa ggattatctc
     1861 ccggccaacg ccacgctcgg tcgcatgggc accaccgaat tcgcggtcat ctgcgacggc
     1921 gaggccgatg cgcgcaccat gttgcgttcg atggtgcgcg cgattaccgg actgcagtgc
     1981 ggcgacaccg atgcgcggat cggcgctcat gccggctacg ccgaggcgcc gcgggacgcc
     2041 accggtagcg acgagctggt gcggcgcgcc cgggtcgcgc tgcgcgcggc gagcgaaaag
     2101 ggatcgggca tcgtcaccgc gttcgagcac gccatcgacg cggtttcgaa ggaccagcaa
     2161 ttcatccgcc gcgaattgcc gcgcgcggtc gcggcggagg cgctggaatt gcactatcag
     2221 ccgatcgtcg cctccgacgg ttcgggccta accggcgttg aagccttgct gcgctggacc
     2281 catcccgagc gcggtgccat cggtccggcg gtctttatcc cggtggcgga acaaatggga
     2341 ctgatcgacc ggttgggcgc ctttgtgctg cgccgcgcgc tgcacgaagc caaacgctgg
     2401 ccggaccttt acatctcggt caatttatcg ccgctgcagg tgcgcgaccc cggcatcgt
//
LOCUS       DASWZT010000164         2283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_20539, whole genome shotgun sequence.
ACCESSION   DASWZT010000164 DASWZT010000000
VERSION     DASWZT010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20539"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VGE92_04440"
     CDS             <1..1033
                     /locus_tag="VGE92_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY1313098.1"
                     /translation="AHSVRNWDYRFCWLRDSFFVILALNHLGATNTMEGYLRYLDNIV
                     ARNPGGDLQPLYSITGEARIDERIVDTLEGYRGMGPVRVGNLAYEQVQHDVYGAVVLA
                     ARQSFFDQRLTNPGNAAVFARAEVLGERALQMFKVPDAGPWEFRGSEHVHTFSAVMCW
                     AACDRLARIASQLQIGDRAAFWRTTADSMRAEILTRAWSERKQSFTSGFGGEELDATS
                     LLLPDLGFIEASDARFVSTLEAIERELVRGDLVFRYLHADDFGVPANTFTMCSFWYVN
                     ALAAVGRVDEARAIFQKLLLRRNSAGLLSEDIDPVSGEQWGNFPQTYSMVGIITCAIR
                     LSRTWEEML"
     gene            complement(1059..>2283)
                     /locus_tag="VGE92_04445"
     CDS             complement(1059..>2283)
                     /locus_tag="VGE92_04445"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEY1313099.1"
                     /translation="AARAAALTLAQTSTEQKNRALNAAAAALRTQRERILAANEADMQ
                     AAAARGTSGALLDRLRLDEPRVAAMARGLEDIARLDDPIGKRLAQWTRPNGMRIERVA
                     VPLGVVGIIYESRPNVTADAGALCLKSSNAAILRGGSESSRSNAAIHACLTAGLDAAG
                     LPQTCIQWVPTVDRAAVGHMLADMRDSIDVLVPRGGKNLIARVRQEARVPVIGHLEGV
                     CHVYVDRGANLEMAKLIVRNAKMRRTGICGAAETVLIDRACAATHLAPILRTLLDAGC
                     EVRGDEMSMRADPRVKPATEQDWYAEYLDAIIAARVVDDEAAAIAHIRRYGSQHTECI
                     VTDDAAAAERFLSTVDSAIVLHNASTQFADGAEFGMGAEIGISTDKFHARGPVGVEQL
                     TSYKYVVRGSGQIRE"
BASE COUNT          383 a          742 c          724 g          434 t
ORIGIN      
        1 ggcacacagt gtgcgcaact gggattaccg gttctgttgg ctgcgcgaca gcttcttcgt
       61 gattctggcg ctcaatcatc tcggcgcgac caacaccatg gaaggctatt tgcgctatct
      121 cgacaacatt gtggcgcgca accccggagg ggatctgcag ccgctgtaca gcatcaccgg
      181 cgaagcccgt atcgatgaga gaatcgtcga tacgctggaa ggctatcgcg gcatggggcc
      241 ggtgcgcgtc ggaaatctcg cttacgagca agttcagcac gatgtctatg gcgctgtggt
      301 gctcgccgcg cggcagtcgt tcttcgatca gcgtctcacc aatcccggca acgcagcggt
      361 tttcgcgcgc gccgaagtgc tgggcgagcg ggcactgcag atgttcaagg tgcccgacgc
      421 gggcccgtgg gagtttcgcg gtagcgagca cgtgcacaca ttttcggcgg tcatgtgttg
      481 ggccgcctgc gaccgtctgg cgcgcatcgc gagtcagctg caaatcggcg atcgcgccgc
      541 attctggcgc accaccgccg actcgatgcg cgcggaaatc ctgacgcgcg cctggagcga
      601 acgcaagcaa tcgtttacca gcggctttgg cggcgaggaa ctcgatgcca cctcgctgct
      661 tctgcccgat ctgggcttca ttgaagcaag cgatgcgcgc ttcgtctcca cgctcgaggc
      721 gatcgagcgc gaattggtgc gcggcgattt ggtgtttcgc tatttgcatg cggatgactt
      781 cggcgtcccc gccaatacat tcacgatgtg ctcgttctgg tacgtgaatg cgctggcggc
      841 ggtcgggcgc gtcgacgaag cacgcgcgat ttttcaaaaa ttattgttga gacgcaacag
      901 cgcgggtttg ctgtccgagg acatcgaccc ggtgagcggt gaacagtggg gaaatttccc
      961 ccaaacctac agcatggtcg gcatcatcac ctgcgccatc cgcttgagcc gcacgtggga
     1021 ggaaatgctc tagccccgcc gccgcgatgc gctcggcttt actcgcgaat ctgtccgctg
     1081 ccgcgcacca catacttgta gctggtgagc tgctcgacgc cgacgggacc gcgcgcatgg
     1141 aatttgtcgg tgctgatgcc gatctccgcg cccatgccga attccgcgcc gtcggcaaat
     1201 tgtgtggacg cattgtgcag tacgatggcg ctgtcgaccg tggacagaaa ccgctcggcc
     1261 gccgcggcgt catcggtcac gatgcactcg gtatgctgcg agccatagcg gcgaatgtgc
     1321 gcgatcgcgg cggcttcgtc atcgaccacg cgcgcggcga tgatcgcatc gagatactcg
     1381 gcgtaccaat cttgttcggt ggccggcttc acgcgcgggt cggcgcgcat gctcatctca
     1441 tcaccgcgca cttcacaccc cgcgtcgagc aaggttcgaa gaatgggcgc caaatgcgtc
     1501 gccgcgcagg cgcgatcgat caacacagtt tccgccgcac cgcagatccc ggtgcgccgc
     1561 atcttggcgt tgcgcacgat gagtttcgcc atctcgagat tggcgccgcg atcgacgtac
     1621 acatggcaga ccccctcgag atgcccgatc acgggcacgc gcgcctcctg ccgcactcgc
     1681 gcaatcagat tcttgccgcc gcgcggcacg aggacatcga tgctgtctct catatcggcc
     1741 aacatgtggc caaccgctgc gcgatcgacg gtcggcaccc attgaataca ggtctgcggc
     1801 agtcccgccg cgtcgagccc cgccgtcagg caggcgtgaa tcgcggcgtt cgaccgcgag
     1861 ctctccgagc cgccgcgcag aatcgccgcg ttgctggatt tgaggcacag tgcgcccgca
     1921 tctgcggtga cgttggggcg gctctcgtaa atgatcccga ccacgcccaa cggcacggca
     1981 acgcgttcga tccgcatgcc gttcggccgc gtccactgcg ccaagcgttt gccgatgggg
     2041 tcgtcgagac gcgcgatgtc ctcgaggccg cgcgccatgg ctgcgacgcg cggctcatcc
     2101 agacgcaggc gatcgagcag cgcccccgac gtgcctcgcg ccgcagcggc ttgcatatcg
     2161 gcttcgttgg cggccaagat tcgctcgcgt tgagtccgca gcgccgccgc ggccgcgttc
     2221 aacgcgcgat ttttctgctc ggtggaagtc tgcgccagag tgagtgcggc ggccctcgcc
     2281 gcc
//
LOCUS       DASWZT010000165         2733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_78621, whole genome shotgun sequence.
ACCESSION   DASWZT010000165 DASWZT010000000
VERSION     DASWZT010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78621"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VGE92_04450"
     CDS             complement(<1..688)
                     /locus_tag="VGE92_04450"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEY1313100.1"
                     /translation="MSIRKQIAAPLFFSALLTCACVPELGVAGAREEGRLLTATEVVE
                     EVQAMPDQQIPDALLSRAYGIAVIPDVTKVAFIFGGRHGNGVLVVRDKLTSAWSNPSF
                     VSLTGGSWGFQAGAQSSDIVLVFTTKTGIEGIAGGKLTLGADASVATGPVGRQGSAAT
                     DMSFNAEIYSYARTRGLFGGIALDGSVISIDRSANSALYGKSGVTATDIFSGQAPAAP
                     PTAQRFLDRLA"
     gene            complement(738..>2733)
                     /locus_tag="VGE92_04455"
     CDS             complement(738..>2733)
                     /locus_tag="VGE92_04455"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein
                     kinase family protein"
                     /protein_id="HEY1313101.1"
                     /translation="SHTLPVTRLQERILTPENAAPEQVLGRPITTATDIYALGVLLYQ
                     LLTGRSPYRLLSYSQLQLERAICMDDPVRPSQMVISKLSGEKDADRSRISDRRGLSPQ
                     RLRARLSGDLDAIIAMAMRKEPERRYPSVEALADDLNRHLNGQPVRARQGDWRYHTVK
                     LLRRHVWAVAAIAAAFLGLALFGGAMLWENHRIQLARDASAQERDRAREVSAFLVDVF
                     SQADPLHAQGKEATAKDLLDRGAEKIKGNLTLQPEVRAQLLESIGLAYRRQGLSERSI
                     PLFEEAVAIRRQERPLDNGKIAVAIANLARALTDSGHLISAEANLQQAVTLTETGGRQ
                     DSVETADILVQFGNFALDAKSDPARAAQLFNQALELYRSALGPQNLQVASALNGLAAA
                     TAWMSDFPLAEHYQREALDIFQATVSRTHPDNAVALATLGSILTQRGKYAEAEQVLNE
                     ALQIERSVFGPDHPRIASIEADLGVMYDREGDLTRAIAATETALRITRERRGASHYTV
                     GYYLDSLATLFLKANDLPAAESDARQALAVYAESLPARHLYVASTRQLLGEVLLRRGS
                     LAAAEAEFRTALDINIGLAGADSWRTARSEASLGWVLIERDRAADGLPMLRAARAKLL
                     ATVGAQHPATQQATARLTEYFHVHHRDAEAAQVLAAPDKL"
BASE COUNT          493 a          867 c          866 g          507 t
ORIGIN      
        1 gcgccaatcg atccaagaac cgctgcgcgg tcggcggcgc cgcgggtgct tgcccggaga
       61 agatgtcggt ggcggtcacg ccgctcttgc cgtacagcgc gctattcgcc gaacggtcga
      121 tggagatgac actgccgtcg agtgcgatgc cgccgaacaa gccacgtgtg cgcgcgtaag
      181 aatagatctc cgcgttgaaa ctcatatcgg tggccgcaga accctgccgt cccaccgggc
      241 cggtggcgac cgacgcatcc gcgcccaagg tcagcttgcc tccggcaatg ccttcgatgc
      301 cggtcttggt ggtgaacacc agaacgatgt cggaggattg cgctccggcc tgaaagcccc
      361 agcttccgcc ggtcagagaa acgaagcttg gattgctcca ggcagaagtc aatttgtcgc
      421 gcaccacgag cacgccattc ccatgacggc cgccgaaaat gaaggcgacc ttggtcacat
      481 cgggaatcac cgcgataccg taggcacgcg acaggagggc gtccggaatc tgctgatccg
      541 gcatggcctg cacttcttcc acgacttcgg tcgccgtcag gaggcggcct tcttcgcgtg
      601 cgccggcaac cccaagctca ggcacgcaag cgcaggtgag cagcgcggag aaaaacaggg
      661 gagcggcaat ctgttttctt atagacatca agactcctag ggttcggacc tggttctggt
      721 cggttagttc ctcaagctta cagcttgtcg ggcgccgcga gaacctgcgc agcctcggca
      781 tcgcggtgat gtacgtgaaa atattccgtc agacgcgcgg tcgcttgctg tgttgcagga
      841 tgctgggcac cgacggtcgc cagtagtttg gcacgggcgg ctcgcagcat gggcaggccg
      901 tcggcggctc ggtcgcgttc aatgagcacc caacccaaac tcgcttcgct gcgtgcggta
      961 cgccaactgt cggctccggc gagtccgatg ttgatgtcga gagcggtgcg aaactccgct
     1021 tccgccgccg cgagcgagcc tcgccgcagc agcacttcgc cgagcagctg gcgcgtcgaa
     1081 gccacatata aatggcgcgc cggcaaggat tctgcataca ccgcaagtgc ctgacgcgca
     1141 tcgctctcgg ccgccggtag atcgttggcc ttgaggaaca gggttgccag tgaatccaaa
     1201 tagtacccca ccgtatagtg gctcgcgccg cgccgctcgc gcgtgatccg caaggcggtc
     1261 tcggtggcgg cgatggcgcg cgtcaggtcg ccttcgcggt cgtacataac acccaaatcg
     1321 gcttcgatgg aggcgatccg cggatggtcc gggccaaaca cgctgcgctc gatttgcaag
     1381 gcctcgttca atacctgctc cgcttcggca tattttccgc gttgcgtgag tatgctgccc
     1441 aaggtcgcga gtgccaccgc gttatccgga tgcgtgcggc tcaccgtcgc ttggaaaatg
     1501 tccagtgctt cgcgctggta atgctccgcc aacgggaaat cgctcatcca cgccgttgcg
     1561 gccgcgagac cattgagcgc ggaagcgact tgcaggttct gtggaccaag cgcactgcga
     1621 tacaactcga gcgcttgatt gaataactga gcggcgcgcg cgggatcgct cttcgcatcg
     1681 agcgcaaagt tgccgaattg aaccaagata tccgccgttt cgaccgagtc ttggcggccg
     1741 ccggtctcgg tcaaggtcac cgcctgttgc agattggcct ccgccgatat caggtgaccc
     1801 gaatcagtga gggcgcgtgc gagattcgcg atcgccaccg caattttgcc attgtcgagg
     1861 gggcgttcct gacggcgtat cgcgaccgcc tcctcgaaca gcggaatcga acgctcgctc
     1921 aagccctggc gccgataggc gagaccgatg ctttcgagaa gctgtgcacg cacttccggc
     1981 tgcagggtca ggttgccttt gattttctcg gcgccgcggt ccaacagatc cttggccgtc
     2041 gcctctttgc cctgagcgtg caaagggtcg gcttgcgaga acacatccac caagaaggca
     2101 gacacctcac gtgcgcggtc gcgttcctgg gcgcttgcgt ccctggccag ctgaatgcgg
     2161 tggttctccc aaagcatggc gccgccgaac agggccagtc cgagaaacgc cgcagcaatt
     2221 gcagccaccg cccatacgtg ccgccgcaac agcttcacgg tgtggtagcg ccagtccccc
     2281 tgccgcgcgc gcaccggctg gccgttgaga tgtcgattca agtcatccgc cagcgcctcc
     2341 accgagggat agcggcgctc gggctctttg cgcatcgcca tggcgatgat cgcgtccaga
     2401 tcgccgctca agcgggcgcg cagccgctgc ggcgatagtc cgcggcgatc ggagatccgg
     2461 ctgcgatccg catccttttc gccgcttagc ttcgagatca ccatttggct cggacgcacc
     2521 gggtcgtcca tgcagatggc gcgttccaac tgcagctgac tgtagctcag gagccgatac
     2581 ggggagcggc cggtcaggag ttggtacagc aatacgccga gcgcatagat gtcggtcgcg
     2641 gtggtgatcg gccggccgag aacttgctcg ggggccgcat tctcgggcgt gaggatccgc
     2701 tcctgcagcc gcgtcaccgg cagggtgtgc gac
//
LOCUS       DASWZT010000166         7314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_59297, whole genome shotgun sequence.
ACCESSION   DASWZT010000166 DASWZT010000000
VERSION     DASWZT010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7314
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59297"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VGE92_04460"
     CDS             complement(<1..393)
                     /locus_tag="VGE92_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313102.1"
                     /translation="MDTLAARQQMIDQQIRTWEVLDPRVLDALSQIPREAFVPPDYRE
                     LAFADAPIPIGFGQSMLAPKLQGRILQALAVNPADSVLEVGSGLGYLSACLSLLGAAT
                     HSIDIHAGLIESARANVAAVAAAKVQFEL"
     gene            560..2860
                     /locus_tag="VGE92_04465"
     CDS             560..2860
                     /locus_tag="VGE92_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY1313103.1"
                     /translation="MSSVEMWSLTSAILVIALVILFAWKRRQERAVNEFAAQVWQLSR
                     EAGAAGRIGLEGKTAALGHLGSAVNQLLENLDERGARLLDREQILQRLVETVHDAVLV
                     HRDRILFANSRFLALLNLSAAEVVGKPLTEFVVPEYLDLVENNLRRRLAGEPAAERYE
                     VELISAQAQVTRVELSSLVIESGGEPALLLTVLEMLPESAQAPVTSRPRAMVTLDAMG
                     ESVVTVDAEGRIDYINHAAETLLGQRFDQVMGKSFSEVAALVDETDRHSLGDPVRKAL
                     TTGGRVTMGRRAVLVPANAGPERSVEISVTPLRFDGKEIIGLVLVLHDTSELRGLTRQ
                     MTYQASHDALTGLVNRREFERRLQEAMDSAQTGIGHALCYLDLDRFKVVNDTCGHTAG
                     DNMLREVASLIKEAVRDSDTVGRIGGDEFALLLVGCPLEKARQIADDVVRSVNEYRFV
                     WKDKIFSIGVSIGLVEIGSGAGGAIEDIMNSADSACYMAKKQGGLHVHVYSAREEASA
                     RHSGEIHWLQKLQGALRDNKFELYYQPIVHARPDGVRGPALEVFVRLEGENGQPGAPP
                     AEFIRAAERFRLMPKIDRWVVQTVLSALGRGGMKLPPGRSVAINIAGQTLGDSDFLAF
                     VVDCFDHTGANPADICFEVTESSVVANLDHAQRFIAVLHGTGCEFALDDFGSGLSSFS
                     TLRTLPMDYLKIDGSFIRNLAGDIVNQAMVAAMIDLSRSLNFRVVAEQVEDQLSLDTV
                     IGMGIDFVQGFVVGRPQPLSMVPSAP"
     gene            2970..3953
                     /gene="cysK"
                     /locus_tag="VGE92_04470"
     CDS             2970..3953
                     /gene="cysK"
                     /locus_tag="VGE92_04470"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002059893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEY1313104.1"
                     /translation="MHVYEDNTKSIGRTPLVRINRLDASSGATILAKIEGRNPAYSVK
                     CRIGAAMVWDAEERGALKPGMSIVEATSGNTGIALAYAAAARGYGCVLTMPETMSMER
                     RKVLVAFGAKLVLTPGAKGMKGAIAKAEELAAADPSRYLLVRQFDNPANPGIHEKTTG
                     PEIWDDTDGKIDVFVSGVGTGGTLTGVSRYIKNTRGKKILTVAVEPVNSPVITQTLAN
                     QPLTPTPHKIQGIGAGFIPKNLDLSLVDRVEQVSNEEAIETARRLAREEGLLSGISCG
                     AAMAVALRLAREPAQKGKTIVVILPDAGERYLSGALFEGLFEGVEKMQAVA"
     gene            3962..4942
                     /locus_tag="VGE92_04475"
     CDS             3962..4942
                     /locus_tag="VGE92_04475"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEY1313105.1"
                     /translation="MKAVAQPPPVAAHEELTQEFLDSYASDQRARRISHRYLPSREAI
                     VEILQGVLDLMYPGYFGRRDLSSENLHAYVAQSLSVLAPKLEREMEHCLCYGREREAA
                     RDEVGRPEFGECAPRARELAGIFLGRLPEIRSLLIRDVQAAFDGDPAATNLDEVILAY
                     PGVLAVSVYRIAHALHDLGVPMMARIMTEWAHSKTGADIHPGANIGPAFFIDHATGVV
                     VGETTHIGEGVKLYQGVTLGALSFPRDASGHIIRGKKRHPTVESGSTLYANATVLGGQ
                     TVVGAESVIGGSVFLTRSVPPRSRVSLKEPELRVLTRDGAAEPDAIYFDI"
     gene            complement(4956..5504)
                     /locus_tag="VGE92_04480"
     CDS             complement(4956..5504)
                     /locus_tag="VGE92_04480"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEY1313106.1"
                     /translation="MFEPAWITEVLHFWFVELGEEHWFAKRPDIDANIRDRFLPLAER
                     LFAEGGSVSSGQRPLLAAVIVLDQFSRNLFRGTPRAFAADSIARRLSKTAIMQGMDVN
                     MTRHERLFLYLPFEHSEDREDQALAIDLIARLGSEEWTRDALAHKEIIDRFGRFPHRN
                     AILGRHSSADELALLEERKQWF"
     gene            complement(5523..7151)
                     /locus_tag="VGE92_04485"
     CDS             complement(5523..7151)
                     /locus_tag="VGE92_04485"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEY1313107.1"
                     /translation="MADVFVSYARGDKARVAPIVAAIEAQGWSVWWDPVITPGQEFDR
                     QIAAELRTAAAVLVVWTPISVASRWVRGEAREGADRGTLVPVRFDAAELPIDVRALHT
                     TELDEWSKDQRSPQIREVLSALGAIIARERKLPPVAANSGAAQAGARSEGDRIAICVL
                     PFTNMSGDPEQEYFSDGVTEDIITELSRWRLLSVRSRSASFRYRGAALDLQQVGRDLN
                     VRFVVEGSVRRLGERIRTSVQLIDAETGNHVWGERFDRRLDEIFAVQDRVVQTIVSTL
                     VGRVQVSDAERARRKPPSSLAAYECVLRANALPWDDPDGAAEAVCLVKKAIELDAGYG
                     FAYALLASLRQQQWSEDADDSAATLDEAYTLAMKSVALDDSESTCHAILGHVCARQRS
                     FELAVQYAQRAIELNPSNQWNAADLGAILVYAGRSEEALTWFSKAREIDPYFDPAWYW
                     RAICLAYMTMRRYADALSNLSHARVRTYRYLALAAGCHAQLGDMDRARASTVECLSLR
                     PDFSILRFLRKEPFTNPADAEQLAASLRLAGLPD"
BASE COUNT         1388 a         2223 c         2340 g         1363 t
ORIGIN      
        1 gagctcgaac tgcaccttcg ccgccgctac ggctgccacg ttcgcccggg ccgactcgat
       61 gagtccggca tgaatgtcga tcgagtgtgt cgctgcaccg agtaaactta agcaggcgga
      121 cagataacct aatccgctgc cgacctccaa gacgctgtcg gcgggattca cggcgagtgc
      181 ttgcaaaatg cggccttgaa gcttgggagc caacatcgat tgaccgaatc cgatgggaat
      241 cggcgcatct gcaaacgcaa gctcacgata gtcgggaggc acgaatgctt cgcgcggaat
      301 ttgcgacagc gcatcgagaa cacgcgggtc gagaacttcc cacgtgcgta tctgctgatc
      361 gatcatttgt tgccgagcgg ccaatgtatc catgatttct cgagggcgaa taaggccgtt
      421 gattatggtc aattttgaca gggtatgcca tgcccgtgcg ctattcttag tcccacgatg
      481 attcgcgacg tgtccaaccc ggcatgggtt ctaagatcaa gcgtcgtatg actcatcgtt
      541 atcgaggtcc gcgttgatct tgtcttccgt cgaaatgtgg agtttgacct cggccatttt
      601 ggtgatcgcc ctggttattc tgtttgcgtg gaagcggcgg caggaacgcg ccgtcaatga
      661 atttgccgcg caagtttggc agctgagccg cgaagccgga gctgccggtc gcatcggact
      721 cgagggcaaa accgcggcac tcggccattt aggttcagcc gtcaaccaat tgttggaaaa
      781 cctcgacgag cgcggcgcac gcctgctcga tcgcgagcag atattgcagc gcttggtcga
      841 gaccgtgcac gacgcggtgc tcgtgcaccg cgatcgcatc cttttcgcca atagccgatt
      901 cttggcgctg ctcaatttga gcgccgcgga ggtcgtcggc aaaccgctga ccgagttcgt
      961 cgttccggaa tacctggatt tggtcgagaa caatctgcgg cgccgcttgg caggggagcc
     1021 cgcggccgag cgctacgagg tggaactgat cagcgcgcag gcgcaggtga cgcgcgtaga
     1081 actctcgagt ctggtgatcg aaagcggcgg cgagccggcg ctgctgctga cggtgctcga
     1141 aatgttgccg gagagcgcgc aggcgccggt cacatcgcga ccgcgtgcga tggttacgct
     1201 cgatgccatg ggcgagagcg tcgttacggt cgatgccgag ggccgcatcg actacatcaa
     1261 tcatgcggcg gagaccctgc tcggccagcg tttcgatcag gtcatgggca agtcgttttc
     1321 ggaagtcgcg gcgctggtgg atgaaaccga tcgccattcg ctgggagatc cggtgcgcaa
     1381 ggcgttgacc accggcgggc gcgtgaccat gggacggcgc gcagtactgg tccccgccaa
     1441 cgcaggcccg gaacgatcgg tggaaatcag cgtcacgccg ctgcgcttcg atggcaagga
     1501 aatcatcggt ttggtgctgg tgctgcacga taccagcgag ctgcgcggcc tcacgcgtca
     1561 aatgacctat caggcgagtc acgatgcgct caccggcttg gtgaatcgcc gcgagttcga
     1621 gcggcgcttg caggaggcca tggattctgc gcagaccggc atcggacatg cgctgtgcta
     1681 cttggacttg gatcgcttca aagtcgtcaa tgacacctgc ggccataccg ccggcgacaa
     1741 catgctgcgc gaggttgcct cgctcatcaa ggaggcggtg cgcgattcgg atacggtggg
     1801 ccgcatcggc ggcgacgagt tcgccttgct gttggtgggc tgcccgctcg aaaaagcgcg
     1861 ccagatcgcc gacgacgtgg tgcgctcggt caacgagtac cgcttcgtgt ggaaggacaa
     1921 gattttcagc atcggcgtca gcatcggctt ggtggagatc ggcagcggcg ccggcggtgc
     1981 catcgaggac atcatgaatt ccgcggattc cgcctgctac atggcgaaaa aacagggcgg
     2041 tttacatgtc catgtgtact cggcgcgcga agaggcaagc gcgcggcaca gcggcgagat
     2101 tcattggctg cagaagctgc aaggtgcgct gcgcgacaat aaattcgaac tctactatca
     2161 gcccatcgtg cacgcccgcc cggacggcgt gcgcggtccg gcgctcgaag tgtttgtgcg
     2221 tctggagggc gagaacggcc agccgggggc gccgcccgcg gaattcatcc gcgcggcgga
     2281 gcgctttcgt ctgatgccaa aaatcgatcg ctgggtggtg cagaccgtgt tgtcggcgct
     2341 gggacgcggc ggcatgaaac tgccgccggg ccgcagcgtc gcgatcaata tcgccggaca
     2401 aacgctcggc gattcggatt ttctggcatt cgtcgtcgac tgtttcgatc atacgggcgc
     2461 gaacccggcc gacatttgct tcgaggtcac ggagagttcg gtggtggcga acctcgatca
     2521 cgcgcagcgc ttcatcgcgg tgttgcacgg caccggctgc gagttcgccc tggatgattt
     2581 cggcagcggc ttgagctcgt tttcgaccct gcgcaccctg ccgatggact acttgaaaat
     2641 cgacggttct ttcatccgga atctggccgg agatatcgtg aatcaggcga tggtcgccgc
     2701 catgatcgac ctatcgcgca gcctcaattt ccgtgtcgtc gccgagcagg tcgaggacca
     2761 gctgtccctc gacaccgtga tcggcatggg catcgacttc gtgcaggggt tcgtggtggg
     2821 ccggccgcag ccgctgtcga tggtacccag cgccccgtag cgacgttccg ccggcgggca
     2881 tataaccccc aggcagaacc tcataacaaa tctatggcat tttcttcctg cttaggagga
     2941 aaatccggcc attgccaatg aggtgaccta tgcatgttta cgaggacaat accaagtcca
     3001 tcggccgcac gcccttggtg cgcatcaacc gtttggacgc gagcagcggc gcgacgattt
     3061 tggccaagat cgagggacgc aaccccgcct actcggtgaa atgccgaatc ggcgcagcca
     3121 tggtgtggga cgccgaagag cgcggcgcgc tgaaacccgg catgtccatc gtcgaggcga
     3181 ccagcggcaa taccggtatc gcccttgctt atgcggccgc cgcccgtggt tacggctgcg
     3241 tgctgaccat gcccgagacc atgagcatgg agcgtcgcaa agtcttggtc gcgttcggcg
     3301 ccaagctggt gctgacgccc ggcgccaaag gcatgaaggg tgcgatcgcc aaggcagagg
     3361 agctggccgc cgccgatccc agcaggtacc tgctggtacg gcaattcgac aacccggcca
     3421 atcccgggat ccatgaaaag accaccggac ccgagatctg ggacgacacc gacggcaaga
     3481 tcgatgtgtt cgtgagcggc gtcggtaccg gcggcacgct caccggcgtg tcgcgataca
     3541 tcaagaacac gcgcggcaag aaaatcctca ccgtggcggt cgaaccggtg aactcgccgg
     3601 tgatcaccca gacgttggcg aaccagccgc tgacgccgac gccgcacaaa atccagggca
     3661 tcggcgcggg attcattccg aagaacctgg acttgtcgct ggtcgatcgc gtcgagcagg
     3721 tcagcaacga ggaagcgatc gagacggcgc ggcgactggc acgcgaagag gggttgctca
     3781 gcggcatctc gtgcggcgcg gcgatggcgg ttgcactgcg tctggcgcgc gagccggcgc
     3841 agaagggcaa gaccatcgtc gtgatattgc cggatgccgg cgagcgctac ttgtcgggcg
     3901 ccttgttcga gggtctgttc gagggcgttg agaaaatgca ggccgtcgcg taagcgcatt
     3961 catgaaagcc gtcgcccaac cgccgcctgt ggccgcgcac gaggagctca cccaggagtt
     4021 cctcgatagc tatgcgagtg atcaacgcgc acggcggatc tcgcatcgct acctgccgtc
     4081 gcgcgaggcc atcgtcgaga ttttgcaagg ggtgctcgat ctgatgtacc ccggctattt
     4141 cggccggcgt gatttgagca gcgagaatct gcacgcctac gtggcgcagt ccctgagcgt
     4201 gctcgcgccc aagctcgagc gcgagatgga gcattgcctg tgctacgggc gcgagcgcga
     4261 agccgcgcgc gatgaggtgg gacgccctga attcggcgag tgcgcaccgc gcgcccgcga
     4321 actcgcggga atcttcctcg gccgcttgcc ggagatccgc agcctgttga tccgcgatgt
     4381 gcaggcggca ttcgacggcg atcccgctgc caccaacctg gacgaagtga ttctggccta
     4441 tcccggcgtg ctggcggtga gcgtgtaccg tatcgcgcat gccttgcacg atctaggggt
     4501 gccgatgatg gcgcgcatca tgaccgaatg ggcgcattcc aagacgggcg ccgatattca
     4561 ccccggggcg aacattggtc ccgctttttt catcgatcat gccacgggag tcgtggtcgg
     4621 cgagaccacc cacatcggcg aaggggtcaa gctgtatcag ggcgtaacct tgggcgcgct
     4681 atcgtttccg cgcgatgcct ccggccacat tattcgcggc aagaaacgcc atccgaccgt
     4741 cgagagcggc tcgacattgt atgccaatgc cacggtgctc ggcggacaga cggtggtggg
     4801 ggccgagagc gtcatcggcg gctcggtgtt cttgacgcgc agtgtgccgc cgcgctcgcg
     4861 cgtgtcgctc aaggagccgg agctgcgcgt gttgacgcgc gacggcgccg ccgaaccgga
     4921 cgcgatttac ttcgacatct gatcggaccg ctcgcttaga accattgctt gcgctcctcg
     4981 agaagcgcga gctcgtctgc agacgaatgt cggccgagga ttgcattacg gtgcgggaag
     5041 cggccgaatc gatcgatgat ctccttgtga gcgagtgcat cacgggtcca ctcctcgctg
     5101 cccaaacgcg cgatgagatc gatcgcgagc gcctgatcct cgcgatcctc gctatgctcg
     5161 aacggcaggt agaggaacaa tcgctcgtgc cgcgtcatat tgacgtccat gccctgcatg
     5221 atcgcggttt tggacaatcg acgcgcgatc gaatcggccg cgaacgcccg tggtgtaccg
     5281 cggaacaggt tgcgcgagaa ttggtcgagg acaatgacgg ccgccagcag cgggcgttga
     5341 ccgctcgaga ccgagccgcc ttctgcgaat aagcgctcgg ctaacggcag aaagcgatcg
     5401 cggatgtttg catcgatgtc cggtcgtttc gcgaaccaat gctcctcccc cagttcgaca
     5461 aaccagaaat gcaagacctc ggttatccag gctggctcga acatgatcct cgtgcgccgt
     5521 gttcaatccg gaaggccggc caggcgcaga gaggcagcga gttgctcagc atcggccgga
     5581 ttcgtgaatg gctccttcct gaggaatcgt aggatcgaga aatccggcct caaggacaga
     5641 cactcgactg tgctggctct ggcgcggtcc atgtcgccca gctgggcatg gcatccggcg
     5701 gcaagcgcaa ggtagcgata ggtgcgaacg cgcgcatggc tgaggttcga caaggcatcg
     5761 gcatagcgac gcatggtcat gtacgccaga cagattgcgc gccaatacca tgccggatcg
     5821 aagtaggggt cgatctcgcg cgcctttgaa aaccaagtca gcgcctcttc ggaccgccct
     5881 gcatagacta aaatagctcc caggtcggct gcgttccatt ggttgctggg attgagctca
     5941 attgcccgct gcgcgtattg cacagccaac tcgaaggagc gttgccgcgc gcacacatga
     6001 ccgaggatgg catggcaggt gctctcgctg tcatcgagcg cgacggactt catggcaagg
     6061 gtgtacgcct catccagcgt ggcggccgaa tcgtccgcat cttcgctcca ttgctgctgg
     6121 cgaagcgaag ccagtaatgc gtacgcgaat ccatagccgg cgtcgagttc gatggctttc
     6181 ttgaccaggc ataccgcctc cgctgcgccg tccgggtcgt cccagggcag tgcattcgcc
     6241 cgcaatacac actcgtaggc tgccagactc gacggcggct tgcgtcgtgc acgctccgca
     6301 tccgagacct gtacccgacc caccaacgtg ctgacgatcg tctgcacgac ccggtcttga
     6361 acggcaaaga tttcgtccag gcggcggtcg aatctttcgc cccagacatg gttgccggtt
     6421 tcggcatcga tcaactgcac gctggtccga attcgctcgc ccaatcgccg gacgctgcct
     6481 tcgacgacaa aacgcacgtt caagtcgcgg ccaacctgct gcaagtcgag cgcggcgccg
     6541 cgatagcgga aggacgccga gcgggatcgc accgacaata gccgccagcg cgacagctcg
     6601 gtaatgatgt cctcggtgac accgtcgctg aaatactcct gttccgggtc accgctcatg
     6661 ttggtgaaag gcagcacgca gatcgcgatg cgatcgccct cgctgcgggc gcccgcttgt
     6721 gccgcaccgg agtttgccgc gaccggcggc aacttacgct cacgagcgat gatcgcgccg
     6781 agcgcgctca gaacttcccg gatctgcgga ctgcgctggt cctttgacca ctcatcgagc
     6841 tcggtcgtat gcagcgctcg cacgtcgatc ggcaactcag cggcatcgaa gcgcaccggg
     6901 acaagggtgc cacggtccgc gccctcgcga gcctcgcccc gcacccatct ggaagccacc
     6961 gagatcggtg tccacacgac gagcaccgcg gcggccgttc tgagttcggc ggcgatctgc
     7021 cggtcgaact cctgacccgg cgtgatcact gggtcccacc acaccgacca gccttgggct
     7081 tcgatcgccg ccacgatcgg cgcaacgcgc gctttgtcgc cgcgtgcgta ggaaacgaag
     7141 acatccgcca tgctggttgg accccggact ggtgatggta ctgcgtttag cgtgttcccc
     7201 agggcttagt gtagcgccac gcggagggcg ggcagtagtc atcacatcat cttgccgagc
     7261 accggcacca acaacagcgc cacgatgttg atgatcttga tcaagggatt gatt
//
LOCUS       DASWZT010000167         5320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_20547, whole genome shotgun sequence.
ACCESSION   DASWZT010000167 DASWZT010000000
VERSION     DASWZT010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20547"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            403..528
                     /locus_tag="VGE92_04490"
     CDS             403..528
                     /locus_tag="VGE92_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313108.1"
                     /translation="MWRYSGDIVFASSSVAQLAEQTLTLTGDPTSGYTGAVHIGA"
     gene            complement(539..1024)
                     /locus_tag="VGE92_04495"
     CDS             complement(539..1024)
                     /locus_tag="VGE92_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313109.1"
                     /translation="MRKLLKLLHEVGAVGVVGSFAACLILVAHAPAPSRSLVAYAAVR
                     QDIASITRWLLVPSLALVLISGLLAIAAHDGYKNAAWAWMKALLGISMFEGTLLTVGA
                     GARRAAELSALAVSGRVDGAQLAQILHGEWGGLWLLLGLSIVNIILAVWRPRLYRRQT
                     A"
     gene            1217..2401
                     /locus_tag="VGE92_04500"
     CDS             1217..2401
                     /locus_tag="VGE92_04500"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEY1313110.1"
                     /translation="MSAPVRALAETLVGRDGPPARAFDNLILTLIVLSVVSVVLQATT
                     ALPGWAAQMLRITEFVVVAVFTFEYLLRIVAAPHKLAFVFSFYGLVDLISIAPFYLAR
                     LDVRYVRILRLLRLLRVLKLQRRILETTVTERTRELAERNLALVQAQQQLTAELDIAR
                     ALQIAILPSAFPAKPGCDGAARMVPASTMGGDFYDFIELPNGQVGLVMADVSGKGVPA
                     AFFMAVSRTNLRELAIHHPDPGDCLACANEVLCTQNPMDLFVTVFYCIFDPATGILRY
                     ANGGHNPPLVRRAGGSLEMLDQMGGLVLGAMPGVTYSNHQVQLRHGDRLVLYTDGVTE
                     AFNSAEEAFGMERLIGEISAHGAGSASELVDRICRSVANFAGDAPQSDDITLTVLAWY
                     AS"
     gene            2398..2748
                     /locus_tag="VGE92_04505"
     CDS             2398..2748
                     /locus_tag="VGE92_04505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY1313111.1"
                     /translation="MTVGVRLESAGPITLMVAVGRLDFGACAGFQLAIDKALAGAGTP
                     AAAVLIDCAGLDYVSSGGLRVFLQSMRAAQRAGIKFALCTLKPAVAEVFELSGFSGII
                     PVHPDRPAALAQMP"
     gene            2745..3149
                     /locus_tag="VGE92_04510"
     CDS             2745..3149
                     /locus_tag="VGE92_04510"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1313112.1"
                     /translation="MNERHTTVPSDAAQLPALTQFLQDFWSANQLPATEAPKFELALE
                     EVFMNVVMHGSQASQPRVDVHLALSSAGITLVVEDEGPAFDPLSVPPPDLAAIEQRPV
                     GGLGVFLVRQLMDTVEYQRVRSRNRLRMTKRI"
     gene            3197..3454
                     /locus_tag="VGE92_04515"
     CDS             3197..3454
                     /locus_tag="VGE92_04515"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY1313113.1"
                     /translation="MSGRLDATQCDAALQFLNAVAEPRLIDLGGLEYIASAGLRVILL
                     TQKRCKAAGTKFRLINAAGPVYDIFHYAGFDQILDISRAGA"
     gene            3465..4481
                     /locus_tag="VGE92_04520"
     CDS             3465..4481
                     /locus_tag="VGE92_04520"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HEY1313114.1"
                     /translation="MTTTLLIRAPLALAPVLLFLAALVHGDSYKLVRLRTVLALLAAG
                     AAAAGASYAANFYWYAQFAGNFDAYSRVVSPWIEEALKAALLVYLIRARRVGLPVDAG
                     IAGFAIGTGFALVENLYYLASRPDTAIAIQVIRGFGTAIMHGGTATVLAIISVTLYER
                     RLNSGLYLFLPGFLAAVAIHSGFNYLLVRPVVATLAILLVLPLIIYAVFQNSDRSLRN
                     WLDEDLDSKVQLLDLINSGTFLESNAGRMLQTLRSRFRGEDLADMLCLLRLNGELALR
                     AKGVLLLRESGIEEPPVDAETRDKLAELNQLERAIGLTGMLAVRPLIMATGKDIWQLR
                     LLGR"
     gene            4609..5178
                     /locus_tag="VGE92_04525"
     CDS             4609..5178
                     /locus_tag="VGE92_04525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY1313115.1"
                     /translation="MRLSRVIVYPETDLELVQRYRGGDRAAFTELVTRYQTPIYNAAF
                     GVLHRADDALDITQEVFLKVAQRLDEYDPRFKFFSWIYRIALNESLNLQRRNNRKAPL
                     DAESGVPAADGANPEKQVSDAQLSRRIQSAMTRLPENDRIVLSLRHFSECSYQSIGQI
                     LDLDEKTVKSRLFESRQRLRALLADLESH"
BASE COUNT          957 a         1615 c         1666 g         1082 t
ORIGIN      
        1 gcacttaccg cagccgcatc ttggagaaga tgggattttc aacgaatgcc gacatcaccg
       61 cgtatgcact gcggaatgga ataattcagt agcgcaacgc ctcgtgccat acttggcgcc
      121 gaggtgcgcg tttcatgaac aagcccggtg gctcgaattc gggacgcgtc gcgcagagtg
      181 gtctggctcg cgatttcggc gttgcctcgc ccgctgcggc gcggcgcgcg gtattgcgct
      241 ggctggcggc gagcggcgcc gctgcactcg cattgcgtgc cgcaaagacc taccccgcgc
      301 cgcttaacta tctgcgcggg gttcagttca ccgactcagc gggtcaggtg cgctttcata
      361 cggtatttcc tgcctgctac cccgggcgct atccgcatct gcatgtggag atattcaggc
      421 gacatcgtgt tcgcgtcgag ttctgttgcc caacttgcgg agcaaacgct gactttgacg
      481 ggcgatccga ccagcggtta taccggtgcg gtgcacatcg gcgcctgaga atgtcgatct
      541 atgccgtctg ccgccgatac agccgcggac gccacaccgc caaaattatg ttaacgatcg
      601 atagtcccag gagcagccac agtccgcccc attcgccgtg taggatctgc gcgagttgcg
      661 ccccgtcgac ccgacccgac acggcaagag cggagagttc tgccgcgcgc cgtgcgcccg
      721 ctccgaccgt gagcagcgtt ccttcgaaca tgctgatgcc gagaagcgcc ttcatccacg
      781 cccacgctgc atttttgtag ccgtcatggg ccgcaatcgc gagtagcccg ctgatcaaga
      841 ccagggcaag cgacggaacg agcagccagc gtgtgatcga agcgatatcc tgcctcaccg
      901 ccgcgtaagc gacgagagaa cgggatggcg cgggcgcgtg ggccaccagg atcaggcaag
      961 cggcaaagga acccacgacg cctaccgcac cgacctcatg gagcagcttg agtagcttcc
     1021 tcacggcgtc gacattgctc gggcgggcat cttgtatggt taccacccgg cgcgcggcat
     1081 cgcaagtcag gccggcgtgc cccggctttc gggcaatggc cgctggacag tggatgtgct
     1141 attggtgggt tctgtactga tgtgtaggac attccttgtt cgccaattcg tgcaacccgg
     1201 ccgggcggtc caatccgtga gcgcgccggt tcgggcgctg gcggaaacgc tggtgggtcg
     1261 cgacggcccc cctgcccgcg ccttcgacaa tctgatcctg acgcttatcg tgctcagcgt
     1321 cgtgtcggtt gtattgcagg cgaccaccgc tctgcccggt tgggccgccc aaatgctgcg
     1381 gatcacggag tttgtcgtag tcgcggtctt cacattcgaa tacctacttc gcatcgtggc
     1441 ggcgccgcac aagcttgcgt tcgttttcag tttctacggc ctcgtcgacc tgatctcgat
     1501 cgcacccttc tatctcgcac gactcgatgt gcgctatgtg cggatcctgc gcctgctgcg
     1561 gttgctgcgc gtactgaaat tgcagcgccg cattctcgaa acgacggtaa cagagcgcac
     1621 acgcgaactc gcggaacgta acctcgcgct cgtgcaggcg cagcagcagc tcacggctga
     1681 actggatatc gcgcgagcgt tgcagatcgc cattctgccg tcggcatttc cggcaaaacc
     1741 gggttgcgac ggtgctgcgc gcatggttcc ggccagcacc atgggcggtg atttctatga
     1801 cttcatcgag cttcccaacg ggcaagtcgg actggtcatg gcggatgttt ccggcaaggg
     1861 tgtaccggcg gctttcttca tggcggtgtc gcgcaccaac ctgcgcgaac tggcgataca
     1921 ccatccagac cccggcgact gtctggcgtg cgccaatgag gttctgtgca cgcaaaatcc
     1981 gatggatttg ttcgtcactg tcttttactg catcttcgat ccggcgaccg ggatattgcg
     2041 ctacgccaac ggcggtcaca atccgcccct ggtgaggcgc gcgggaggtt cgctcgagat
     2101 gttggatcag atgggtgggc tggttttggg cgcaatgccg ggggttactt actcgaacca
     2161 ccaggtgcag ctgcggcacg gcgaccggct cgtgctctac accgatggcg taaccgaggc
     2221 attcaactca gccgaagaag cctttggcat ggagcggctc atcggcgaaa tcagcgcgca
     2281 cggcgctggg tccgcatcgg agctcgtcga tcggatctgc cgcagcgtcg cgaatttcgc
     2341 cggcgatgct ccgcaatccg acgacatcac cctcacggtg ctcgcgtggt acgcgtcgtg
     2401 accgtaggcg ttcgtctcga atcggcgggc ccgatcacgc ttatggtcgc tgtgggccgg
     2461 ctggatttcg gtgcctgcgc gggcttccaa ttggcgatcg ataaggcctt ggccggtgcg
     2521 ggaacgccag cggcggctgt gctcatcgac tgtgccgggc tcgattacgt atcgagcggc
     2581 ggtttgcggg tctttttgca atcgatgcgc gccgcccagc gcgcaggcat taagtttgcg
     2641 ctgtgcacgc tcaagcccgc ggtcgccgag gtattcgaac tcagcggctt cagcggcatc
     2701 atccccgtcc acccggaccg ccccgccgct cttgcacaaa tgccatgaac gagcggcata
     2761 cgacggtacc gagcgatgcg gcccaattgc cggcactgac ccaatttctc caggatttct
     2821 ggtcggccaa ccagctgccg gcgaccgagg cgccgaaatt cgagttggca ctcgaggagg
     2881 tcttcatgaa cgtcgtaatg cacggttcgc aagcaagcca gccgcgggtc gacgtgcatc
     2941 tggcgctttc ctcggcaggc attacgctgg tcgtcgagga cgagggaccg gcctttgatc
     3001 cgctgtcggt gccgcccccg gacctggcgg caatcgagca gcgcccggtg ggcggcctcg
     3061 gggtatttct cgtgcggcaa ttgatggaca cggtcgagta ccaacgcgtg aggtcacgca
     3121 atcgactgcg tatgaccaag cggatatgag caaacccggg ttcgagatac ggctcgggtc
     3181 ggcaggcgaa gtcgttatga gcggacgtct ggacgcaacc cagtgcgatg cggctctgca
     3241 gttcttgaat gcggtggccg aaccacggct gatagacctt ggcggactcg agtacatcgc
     3301 gagtgcgggg ctgcgcgtga ttctgctcac gcagaagcgc tgcaaagcag cgggtaccaa
     3361 gtttaggctg atcaacgccg cgggtccggt ctacgatatt ttccactacg ccggcttcga
     3421 ccaaattctc gatatcagcc gcgcgggggc ctagcagaat cgccgtgacc acgacgttgt
     3481 tgattcgcgc accgcttgcc cttgcgcccg tgctgttgtt cctggcggcg ctcgtgcacg
     3541 gtgacagcta caagttggtt cgtctgcgaa ccgtgttggc gctgctggcc gcgggtgccg
     3601 ctgcagcggg cgcgagctat gccgccaact tctactggta cgcacagttc gcgggcaact
     3661 tcgacgccta ttcgcgcgtc gtatcgccgt ggatcgagga ggcgctgaag gcggcgctgc
     3721 tggtttactt aattcgtgcg cggcgcgtcg gtctgccggt ggacgccggc atagcgggct
     3781 tcgccatcgg taccggcttc gctctcgtcg agaacttgta ctatctcgcc tcgcgtccgg
     3841 acaccgccat cgcgattcag gtgatccgcg gcttcggcac cgcgatcatg cacggcggca
     3901 ctgcgacggt cctcgcgatc atctcggtca cgctttacga gcggcggctg aacagcggtc
     3961 tttacctctt cctgccggga ttcttggcgg cggtggcgat ccacagcggc ttcaattacc
     4021 tgctggtgcg gcctgtcgtg gcgacgctgg ccattttgct ggttttgccg ctgatcatct
     4081 atgcggtgtt tcaaaacagc gatcgctcgt tgcggaactg gctcgacgag gacctcgact
     4141 ccaaggttca gctgctcgat ttgattaatt ccggcacgtt tcttgagagt aacgcgggac
     4201 gcatgctgca gaccctgcgc agccgattcc gcggggagga cttggcggac atgctctgcc
     4261 tactgcgact aaatggcgaa ctcgccctgc gggccaaagg ggtgttgctg ctgcgcgaga
     4321 gcggtatcga ggagcccccg gtcgatgcgg aaacccgaga caagctggcg gaactcaatc
     4381 agctggagcg ggcgatcggc ctcaccggga tgcttgccgt gcgtccgctg atcatggcca
     4441 ccggtaagga tatttggcag ctgaggctcc tgggacgtta gcaaggtcgg tacgccggca
     4501 aatccgtgtt ttaaccgccg acccgataac ctatgatggt caacataacg ataagggaga
     4561 ccgataggtg ggaaaagccc gacttatcca tccatgtgga gccaactttt gcggctctca
     4621 cgtgtaattg tgtatccgga aacagatttg gaactggtgc aacgttaccg aggcggtgac
     4681 cgggccgcct tcacggaatt ggtaacccgc taccagacgc cgatctataa cgcagcgttc
     4741 ggtgtgctgc atcgggccga cgatgcgctc gacatcaccc aggaagtatt tctcaaggtg
     4801 gcgcagcgat tggatgaata tgacccgcga ttcaagttct tcagctggat ctatcggatt
     4861 gcgctcaatg agtcgctcaa tttacagcgt cgcaacaacc gcaaggcacc cctcgatgcc
     4921 gaatccggtg tgccggccgc cgatggcgcc aatcccgaaa agcaggtaag cgatgcacag
     4981 ctgtcacgaa gaattcagag cgcgatgaca cgcctgccgg aaaacgaccg aatcgtgctt
     5041 tcgctgcggc acttttccga atgcagctat cagtccatcg gacagatcct tgatctggac
     5101 gagaagacgg tgaaatcgcg cctgttcgag tcgcggcaac gattgcgggc gctcctcgcg
     5161 gacttggagt cgcactgatg cagcgtaccg agctgatgca gctgatgcac gaggtcctcg
     5221 acggtgaggc ctcagcgcaa gatgcacacg agctcgaccg ggtattggcg gccgacccgg
     5281 aagcgcgcac tgaatttgag cagctgcggc gcttcttcgc
//
LOCUS       DASWZT010000168         3013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_15737, whole genome shotgun sequence.
ACCESSION   DASWZT010000168 DASWZT010000000
VERSION     DASWZT010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15737"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /gene="zwf"
                     /locus_tag="VGE92_04530"
     CDS             <1..1343
                     /gene="zwf"
                     /locus_tag="VGE92_04530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY1313116.1"
                     /translation="RIFGVARTALSRTEYLFQAESACREFLGKEFDAAHWMGFSRLLT
                     YVKLDAAVAADYAQLKAALKGREAFVRVFFFSTASNIFSVICQNLAKAAVVTALSRVV
                     LEKPLGHDTSSADLINTQVGAVFAEKQIYRIDHYLGKEAVQNLMALRFGNALFEPLWR
                     RGLIKHVQITVAEELGVERRGQFYDKTGALRDMVQNHLLQLLCIMAMEPPASSDPDAV
                     RDEKLKVLRALRPLADRDVLTKTVRGQYKAGAVRGVPVPGYLEEADIAPDSSTETFAA
                     LKVEIDTWRWAGVPFYLRTGKRLQDRLTELVVTFDEVPHPIFEKPPTTRTANELVIRL
                     QPDESITLTILAKNPGEGMRLKPVSLALDLGETFKTRSLDAYERLLMDTVKGNLTLFM
                     RRDELDAAWAWIDPIRAGWEQYDEPPKIYIAGSWGPAASSAMIGRDGFAWHDEL"
     gene            1330..2037
                     /gene="pgl"
                     /locus_tag="VGE92_04535"
     CDS             1330..2037
                     /gene="pgl"
                     /locus_tag="VGE92_04535"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEY1313117.1"
                     /translation="MTNSELPARRFADAEALARELAAQIAASLTAAIGARGLASLVVS
                     GGRSPVRLFEILRTQPLDWAHVCIALADERWVSPEDAASNEHLVRAVLLKEHAAAARF
                     HGMKNGAPTPDLGAVSAWETFARVPRPFDAVVLGMGDDGHTASLFPRSPNLPSALNRA
                     AAAGCVGMWSPLAPQPRLSLNLTALLDARRIVLLISGEEKWRTYAAACGDGPVEEMPV
                     RAVLRQTHTPVDVMWSP"
     gene            2025..>3013
                     /locus_tag="VGE92_04540"
     CDS             2025..>3013
                     /locus_tag="VGE92_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEY1313118.1"
                     /translation="MVAVSSLRLEDDRTALSKEALKNAFLDDLFYVQGKFPALATQND
                     YYMALSFAVRDRMLQRWISTAAAYTRQGSRTVAYLSAEFLMGPHLGNNLINLGIFDSV
                     KQCMTELGLNFEELLQQEEEPGLGNGGLGRLAACFIESLATLEVPSVGYGIRYEFGIF
                     HQEIVDGWQVEKTDKWLRFGNPWELVRPEWAVEVKFGGSTEQYIDERKRLRVRWVPHK
                     TVIGVPYDTPILGYRSNTANTLRLWRSEAPESFDFAIFNSGDYYGAVNQKVASENLSK
                     VLYPNDEQLRGKELRLEQQYFFVSCSLQDMLRILRTQKLPVEQFHVKFAVQLN"
BASE COUNT          571 a          948 c          956 g          538 t
ORIGIN      
        1 cgcgcatttt cggcgtcgcc cgcacggcac tgtcgcgcac cgaatacttg tttcaagccg
       61 aatccgcttg ccgcgaattc ctcggcaagg agtttgacgc agcgcactgg atggggttca
      121 gccgcctgct cacctacgtg aagctcgacg cggcggtcgc ggcggactac gcgcagctca
      181 aggctgcact caaggggcgc gaagcattcg tccgcgtatt tttcttctcc accgcatcca
      241 atatattttc ggtgatctgt cagaacttgg ccaaagcggc ggtcgtgacc gcgctttcca
      301 gggtggtgct ggagaaaccg ctgggacacg atacgtcctc cgccgatctg atcaacaccc
      361 aggtcggcgc ggtatttgcg gagaagcaga tctatcgtat cgaccactac ctgggcaagg
      421 aggccgtgca gaacctgatg gcgctgcgct tcggcaacgc cttgttcgaa cctttgtggc
      481 gccgcggatt gatcaagcac gtgcagatca ccgtggccga agagctcggc gtcgagcggc
      541 gcggtcagtt ctacgacaag accggcgcat tgcgcgacat ggtgcagaat cacctgttgc
      601 agctgctgtg catcatggcc atggaaccgc cggcgagcag cgacccggac gcggttcgcg
      661 acgagaaact caaggtgctg cgcgccttgc gtccgctcgc cgatcgagat gtgctgacca
      721 agaccgtccg cggccagtac aaggccggcg ccgtcagggg cgtgcccgtg ccgggctatc
      781 tcgaagaggc cgacatcgcg cccgacagca gcaccgagac attcgcggcg ctcaaggtgg
      841 aaatcgacac ctggcgctgg gccggagtgc cgttttattt gcgcaccgga aaacgcctgc
      901 aggatcgctt gaccgaattg gtggtgacct tcgacgaggt accgcatccg atcttcgaga
      961 agccgccgac cacgcgcacc gccaacgaac tcgtgatccg gttgcagccg gacgaaagca
     1021 ttaccttgac gatattggcg aaaaaccccg gcgagggaat gcgcctgaag cccgtgagcc
     1081 tggcgctcga cttgggagag acattcaaga cccgcagcct ggacgcgtac gagcggctgc
     1141 tcatggatac ggtgaagggg aatctcacat tattcatgcg ccgcgatgag ctcgacgccg
     1201 cctgggcgtg gatagacccc atccgtgccg gctgggaaca atacgacgaa ccgccgaaga
     1261 tctacatcgc cggcagctgg gggcccgcag cctcgagcgc gatgatcgga cgcgatggat
     1321 ttgcctggca tgacgaactc tgaactccct gcgcggcgct tcgcggatgc cgaggcactg
     1381 gcgcgcgaac tcgcggcgca aatcgccgcg agtctcacgg ctgcgatcgg cgcccgcgga
     1441 cttgcgagtc tggtggtatc gggcggccgc tctccggtac ggctattcga gatcttgcgc
     1501 acgcaaccgc tcgattgggc gcatgtctgc atcgcgctag ccgacgagcg ttgggtgagt
     1561 cccgaggatg ccgccagcaa cgaacatctg gtgcgcgcag tgctgctcaa ggaacacgcg
     1621 gccgctgcgc gcttccatgg catgaagaac ggcgctccga ccccggatct cggggccgtg
     1681 tcggcttggg agacattcgc acgggtgccg cgacccttcg atgcggtcgt gctcgggatg
     1741 ggcgacgatg gacacacagc ctccttgttt ccgcgctcgc cgaatttgcc gagcgcgttg
     1801 aatcgcgccg ccgccgccgg ctgcgtcggc atgtggtccc cgctcgctcc gcaaccacgc
     1861 ctgagcttga acctgacggc gctgctcgat gcgcgccgca ttgtgctgtt gatcagcggc
     1921 gaggagaaat ggcgcaccta cgctgccgcc tgcggcgacg ggccggtgga ggaaatgccg
     1981 gtgcgcgccg tgctgcgtca aacgcacaca ccggtcgatg tcatgtggtc gccgtgagtt
     2041 cgctgcggct ggaggatgac cgcaccgcct tgagcaagga ggcgctcaag aacgcgtttc
     2101 tcgacgatct gttttacgtg cagggcaaat ttcccgcgct cgccacccag aacgactact
     2161 acatggcgct cagcttcgcg gtgcgcgacc gcatgttgca gcgctggatc agcaccgcgg
     2221 ccgcctacac ccggcaaggc tcgcgtaccg tcgcgtatct atccgccgaa ttcttgatgg
     2281 gcccgcactt gggcaacaac ctcatcaatc tcggcatctt cgacagcgtc aagcagtgca
     2341 tgaccgaact cggattgaat ttcgaggagc tgctgcagca agaagaggag ccggggctcg
     2401 gcaacggcgg tctcggccgc ctggctgcgt gcttcatcga gtccctggcg accctcgaag
     2461 taccctccgt cggctacggc atacgctatg agttcggcat tttccatcag gagatcgtcg
     2521 acgggtggca ggtcgagaag accgacaaat ggctgcgctt cggcaacccg tgggaattgg
     2581 tgcggcccga atgggcggtc gaagtgaagt tcggcggcag caccgaacaa tacatcgacg
     2641 agcgcaagcg cctgcgcgtg cgctgggtgc cgcacaagac cgtcatcggc gtaccctacg
     2701 acacgccgat cctgggctac cgcagcaaca ccgccaatac actgcgcctg tggcgctccg
     2761 aggcgcccga gtccttcgac ttcgcgatct tcaacagcgg cgactactac ggcgcggtga
     2821 atcagaaagt cgcgtccgag aacttgagca aagtgctgta cccgaacgat gagcaactgc
     2881 gcggcaagga gctgcggctc gaacagcagt atttcttcgt ctcgtgctcg ctgcaggaca
     2941 tgctgcgcat cctgcgcacg cagaagctgc cggtggagca attccatgtg aaatttgccg
     3001 tgcagctcaa cga
//
LOCUS       DASWZT010000169         2016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_93221, whole genome shotgun sequence.
ACCESSION   DASWZT010000169 DASWZT010000000
VERSION     DASWZT010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93221"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VGE92_04545"
     CDS             <1..823
                     /locus_tag="VGE92_04545"
                     /inference="COORDINATES: protein motif:HMM:NF018172.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030058 - amine dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amine dehydrogenase large subunit"
                     /protein_id="HEY1313119.1"
                     /translation="FITVYDTQTFNPVGEIVLPGAKRALITAMEGLIAFTDDQRMALV
                     FNFTPASSVTVVDLIKRQVLGEVEIPGCSLVYPSGTRGFSTLCSSGTMLSVRLDPNGA
                     VSGRSESKAFNPLDTDPLFTASTLVGGVRYFPSMHGRVQPIDMRGDEVKILPDWPLVP
                     AADEAGHWRPSGWQIVAGDEQKLLYVLMQPQAHEGTHKDPATEVWVYNTANKTRVKRL
                     RLARPGSSIALTHSTEPLLLVQAGERLDVYETSGSLVRSLDLPGLHTRMQIEPVR"
     gene            823..1356
                     /locus_tag="VGE92_04550"
     CDS             823..1356
                     /locus_tag="VGE92_04550"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEY1313120.1"
                     /translation="MSVTQLLSVQLAVFLAALLLVTGLHKLIQRERIQAVLQDFAGVR
                     PRWATPAVLVVGASELLAGVLLCSASYRAAGAVLAGVLWGGYLGLIVRAIAQGRREVD
                     CGCTLGAAARPLGAYPVARNVALMGMAVFVAVSSARNVALAAGAAQLLAACTLVALYA
                     ALDQVMALAAPRRGELL"
     gene            1353..1967
                     /locus_tag="VGE92_04555"
     CDS             1353..1967
                     /locus_tag="VGE92_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEY1313121.1"
                     /translation="MSFLIASQIMLWIALLVLAVVCMALARQIGVLHQRIAPAGALSL
                     RQPLKLGDPAPEMRLTGLDGVPLQIGGARAGRSQLLLFLSPSCTICEALLPALRSAQG
                     AERGWLDIVLASDGAGEQHAAFVRDKGLGKFPYVVSEQLGRSYGVAKLPYAVLIDEAG
                     KLSSSGLVNTREHLESLFVAKEIGVGSIQQFLGQRAPRHASSGE"
BASE COUNT          324 a          600 c          695 g          397 t
ORIGIN      
        1 tttcatcacc gtctacgaca cgcagacgtt caatccggtc ggcgagatcg tgctgccggg
       61 agcgaagcgc gcgctgatca ccgccatgga aggactgatc gcgttcaccg atgatcagcg
      121 catggcgctg gtattcaatt tcacaccggc ctcgtcggtg accgtcgtcg atctcatcaa
      181 gcgccaggtg ctcggcgagg tcgaaatacc cggctgctcg ttggtctatc cgtcaggaac
      241 gcggggattc tcgactctgt gctcgagcgg cacgatgttg agcgtgcgtc tcgatccgaa
      301 tggcgcggtc agcggccgca gcgagtcgaa agccttcaac cctctcgata ccgatccgtt
      361 gttcaccgcc tcgacgctgg tcggcggagt gcgttatttc ccgagcatgc acggccgcgt
      421 tcagccgatc gacatgcgcg gcgacgaggt gaaaatactg ccggattggc cgctggtgcc
      481 ggccgccgac gaagccgggc attggcgccc gagcggctgg cagatcgtcg ccggcgatga
      541 gcaaaagttg ctgtatgtgc tgatgcagcc gcaggcgcat gagggcacgc acaaggatcc
      601 ggcgaccgag gtgtgggtgt acaacaccgc caacaagacg cgcgtcaaac gactgcggct
      661 ggcacgcccc ggatcctcga ttgcactcac gcacagcacg gaaccgctgt tgttggtgca
      721 agcgggcgag cggctcgacg tctatgagac gagcgggagc ctggtgcgca gtttggattt
      781 gcccgggttg catacccgca tgcaaatcga gccggtgcgg taatgagcgt gacgcagctg
      841 ctcagcgtcc agctcgcggt attcctggcc gcgctgctgc tggtcacggg cctgcacaaa
      901 ctgattcagc gtgagcgcat ccaggccgtg ctgcaggatt tcgccggggt gaggccgcgc
      961 tgggcgacgc cggcggtgct cgtcgtcggc gcgagcgaat tgctcgccgg cgtgctgctc
     1021 tgctcggcct cgtatcgtgc ggcgggtgcg gtgctcgcgg gcgttctctg gggcggctat
     1081 ctcgggctga ttgtacgcgc gattgcccaa ggacggcgcg aggtggattg cggctgcacc
     1141 ttgggcgccg ccgcgcgacc cctcggtgcc tacccggtgg cgaggaatgt ggcattgatg
     1201 ggcatggccg tgttcgtggc ggtgagttcg gcgcgaaatg ttgcgctcgc tgccggtgcg
     1261 gcgcaactcc tcgcggcatg cacgctggtc gcgctgtatg cggcgctcga tcaagtgatg
     1321 gccctggcgg cgccgcgccg cggagaattg ctgtgagctt tttgatcgcc tcgcagatca
     1381 tgttgtggat cgcgctgctc gtgctcgcgg tggtgtgcat ggcgctcgcg cggcaaatcg
     1441 gcgttctgca tcaacgcatc gcgccggcgg gcgccttgtc gctgcggcaa cccttgaagc
     1501 tgggtgatcc cgctcccgaa atgcggctca ccggcttgga tggcgtgccg ctgcagattg
     1561 gcggtgcgcg cgccggacgc agtcaattgc tgctgttcct gtcgccgagc tgtacgattt
     1621 gcgaggccct gttgccggcg ttgcgttcgg cgcagggtgc cgagcgcggt tggctcgata
     1681 tcgtcttggc gagcgacggc gccggcgagc agcatgccgc gttcgtgcgc gacaagggtc
     1741 tcggcaaatt cccgtatgtg gtatcggaac agctgggccg cagctacggc gtcgccaaac
     1801 ttccctacgc ggtattgatc gatgaagccg gcaagctatc ttcgagcggt ctggtgaata
     1861 cgcgcgagca tctggaaagc ctgttcgtcg ccaaggaaat cggcgtcggc agcattcaac
     1921 aatttctcgg tcaacgcgca ccgaggcatg catcttcggg agaataagag tggctcaatt
     1981 cgatcagttg acggaaaaag tattgcgcgg catcgc
//
LOCUS       DASWZT010000170         4520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_35105, whole genome shotgun sequence.
ACCESSION   DASWZT010000170 DASWZT010000000
VERSION     DASWZT010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35105"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VGE92_04560"
     CDS             complement(<1..219)
                     /locus_tag="VGE92_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY1313122.1"
                     /translation="MDLGIRGRSAIVCASSQGLGKACARALAEAGVSLVINGRNRVLL
                     EQTAAEIRDATGAEVTPVLADVSSAEGQA"
     gene            337..684
                     /locus_tag="VGE92_04565"
     CDS             337..684
                     /locus_tag="VGE92_04565"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY1313123.1"
                     /translation="MTIKRIQVGSRMSQAVIHNKTVYLAGQVAKGATVAVQTREILAT
                     IDALLAEANTDKSRLLSASIWLTDMATFAEMNRVWESWVVAGQTPARATVMSAHLAGA
                     EYKIEIAVIAAQP"
     gene            694..1089
                     /locus_tag="VGE92_04570"
     CDS             694..1089
                     /locus_tag="VGE92_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313124.1"
                     /translation="MEPKLVTAPRLAGILKELQSREPIFHRREFGASRRDFENMTDPD
                     FWEVGASGRRYSRKFILDTLDNRLPRGEVDEWQAHGFHCIEVAADNYLITYTLHQGAR
                     VTRRATLWRHSKAGWQALYHQGTVVEAAP"
     gene            1110..1856
                     /locus_tag="VGE92_04575"
     CDS             1110..1856
                     /locus_tag="VGE92_04575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1313125.1"
                     /translation="MLKLQNIYKTFEGKAVLSDVNLTVPKGATHALIGSSGSGKTTLL
                     RITLGLIPFDKGYVKINDQALLSFTPAEWADRIGYVPQDGGLFPHISGKHNIALIAGL
                     RGWSKSKIDERVEELRKVVNLDPEILGHFPREMSGGQKQRVAIMRAAMMDPAVMLLDE
                     PMAALDPLIRRSLQQELKSIFQRLGKTVLLVTHDLGEAVYLAEQITMLHEGKVVQTGS
                     YRDLLLTPANAFVGQFINAQRTLPDAGDLK"
     gene            1853..3343
                     /locus_tag="VGE92_04580"
     CDS             1853..3343
                     /locus_tag="VGE92_04580"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY1313126.1"
                     /translation="MKPWLGALWLGMGYFGCGGVCAADSIVIGGKIFTEGYVLGEIAA
                     QTIEASSQVPVTRKLGMGSTGILFEALKSDAIDVYADYTGTLAEAILKRPELRSLDEI
                     RAALAGLGLTMSGSLGFNDTYALAVKEEFAQKYALHTIGDLVSIQSRVRAAFSYEFMD
                     RKDGYPGLVDSYHLGFNPQQVNRMEHSLSFQALEQNAVDLIDVYSTDAKIEKFHLRLL
                     TDDHNYFPVYQAVWVARRAFVDQHPQEWQALLRLEGKISEEAMRNMNAQADIHKVSFE
                     KIAAQFLGAEAPAGKGWGREIARRTREHLWLVGVSLLFSVLVGIPLGVTAVRFHAAGQ
                     AILLSSAVVQTIPSLALLCFLIPVFGVGTKPALAALCLYSLLPVVLNTFTGIRAVNPI
                     HAENARAFGLNRRQVLFRVVLPLASPTLLAGIKTATIVSIGTATLAALVGAGGYGAPI
                     VSGLALNDVPTILTGAIPAALMALVAHGIFELLGLVLIPAGLRRRS"
     gene            3463..>4520
                     /locus_tag="VGE92_04585"
     CDS             3463..>4520
                     /locus_tag="VGE92_04585"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY1313127.1"
                     /translation="MIRVSVTTWVIELGSSTMPQSQAASEGVSNFAAVPGLNRFTAAG
                     VAVTALLSSARADVDAAAGDQPALPSVEVNGTAIQFERDISQSINTVDKKELSEQNLT
                     LVSDALRNVPGITLNSGEGGAHGDSVNLRGLSIPDSFFMDGVRDIGQYQRDTFNSDAI
                     SVLLGPASAVFGRGSTSGVINGISKQPLLAPLASLSVSAGQAHLWRETGDFNLPLSST
                     SALRLAVMDQRNGVEQRDEVLYRRYGVAPTLAFGIETATRLTLSYFKEEENNIPDYGI
                     PFIDGAPANVARSNYYGLLNYDRTRTNSNIGTLRFEHDFSDSITFSDSLRYANYGFEY
                     LVSGPFLGNDSVAPPPPDT"
BASE COUNT          861 a         1334 c         1450 g          875 t
ORIGIN      
        1 ggcttggcct tcggcgctgg agacgtcggc aagcaccggc gtgacttcgg cgccggtggc
       61 gtcgcggatt tccgccgcgg tttgctcgag cagtacgcga ttgcgtccgt tgatcaccaa
      121 tgagacgccc gcctcggcga gcgcccgcgc gcaggccttg ccaagtccct gactcgaggc
      181 acaaacgatg gccgagcgcc cgcgaattcc caaatccatg gcctctcctc aaatggctgc
      241 gttgtcagaa ttacaatagc gccggcgcgc ttgcattgcg cgcgaatcga tcgtatagcc
      301 cttctggcgg cataggcaaa cgcaaaggga gcctcgatga ccatcaaacg tattcaagtc
      361 ggatcgcgca tgtcgcaggc cgtgatccac aataagaccg tatacctggc gggtcaggtt
      421 gccaaaggcg ccacggtcgc ggttcaaacg cgggaaattc tcgcgaccat cgatgcgctg
      481 ttggcggagg caaacaccga caagagccgg ctgctgtcgg cgagcatctg gctcacggac
      541 atggcgacgt tcgcggaaat gaaccgcgtg tgggagtcgt gggtggttgc ggggcaaacg
      601 ccggcgcgcg ccacggtcat gagcgctcat ttggcgggcg ccgaatacaa gatcgaaatt
      661 gccgtgatcg cggcccaacc gtaagggttt ttcatggaac ccaagctcgt tactgcgccg
      721 cggctcgccg gcattctcaa ggaattgcaa agccgcgaac cgatttttca ccgccgcgag
      781 tttggcgcga gccggcgcga tttcgagaac atgacggatc ctgatttttg ggaagtgggc
      841 gcctcggggc ggcgttactc gcgcaagttc atcttggata cgctcgacaa ccgtctgccg
      901 cgaggtgagg tcgacgagtg gcaggcacat ggatttcatt gcatcgaggt cgccgccgac
      961 aattatttga tcacctacac gctgcatcaa ggtgcgcgcg tcacgcgccg tgcgacgttg
     1021 tggcgccatt cgaaggccgg ttggcaggct ctatatcatc agggcaccgt ggtcgaggcg
     1081 gcgccgtaag acccaaacga tggaccgtca tgttgaagct gcagaatatc tacaagacct
     1141 ttgaaggcaa agccgtcctg agcgacgtga atttgacggt tccgaagggt gcgacgcatg
     1201 ccttgatcgg ctcgagcggc tcggggaaaa ccactttatt gcgcatcacg ctcggtttga
     1261 tcccgttcga caagggctac gtcaagatca acgatcaggc gctgttgtcg ttcacgccgg
     1321 ccgaatgggc cgatcgtatt ggctatgtcc cgcaggacgg cggcctgttt ccgcacatca
     1381 gcggcaagca caacatcgcc ttgattgcgg ggctgcgcgg ttggagcaag tcgaagatcg
     1441 acgagcgggt ggaggaattg cgcaaggtgg tgaacttgga cccggaaatt ctcgggcatt
     1501 tcccgcgcga gatgagcggc ggccagaagc aacgggtcgc gatcatgcgt gccgcgatga
     1561 tggatccggc ggtgatgctg ctcgatgagc cgatggcggc gttggaccct ctgatccgcc
     1621 gcagcttgca gcaggaactt aagtcgatat ttcagcgcct cggcaagacg gtgctgctgg
     1681 tcacacacga tttgggcgag gcggtgtatc tggccgagca aatcaccatg ttgcacgaag
     1741 gcaaggtggt gcagacgggg tcttaccgcg acctgttgct cacgcccgcc aatgccttcg
     1801 tcggccagtt catcaatgcg cagcgcacgt tgccggatgc gggtgacctc aagtgaagcc
     1861 gtggctgggc gcgctgtggc tggggatggg gtatttcggg tgcggcggcg tgtgcgccgc
     1921 cgacagcatc gtcatcggcg gcaagatctt caccgagggt tatgtgctcg gcgagatcgc
     1981 ggcgcaaacc atcgaggcct catcccaggt tccggtgaca cgcaagctcg ggatgggcag
     2041 caccggcatt ttgttcgagg cgttgaagag cgatgcgatc gatgtgtatg cggactacac
     2101 ggggaccttg gcggaggcga ttctcaagcg cccggaactg cgctcgctcg atgagattcg
     2161 agcagccctc gcgggcttgg ggctgaccat gtccgggtcg ctgggattca acgacacgta
     2221 cgcgctcgcg gtgaaagagg agttcgcgca gaaatacgca ctgcacacca tcggcgatct
     2281 ggtgtcgatc caatcgcgtg tcagggcggc cttcagctac gaattcatgg atcgcaagga
     2341 tggatatccc ggcctcgtcg acagctatca tctcggcttc aacccgcagc aagtcaaccg
     2401 catggagcat tccttgagct ttcaggcgct cgagcagaat gccgtggatc tgatcgacgt
     2461 gtattcgacc gatgcaaaaa tagaaaaatt ccacctgcgt ctgctcacgg acgatcacaa
     2521 ttattttccg gtgtatcagg cggtgtgggt ggcacgcagg gcgttcgtgg accagcaccc
     2581 gcaggaatgg caggcgctgt tgcggctgga aggcaagatc agcgaagagg cgatgcgcaa
     2641 catgaacgcg caggccgaca ttcacaaggt cagcttcgag aagattgccg cgcagtttct
     2701 cggcgccgaa gcacctgcgg ggaaaggctg gggtcgcgaa atcgcgcgcc gcacccgcga
     2761 gcatctgtgg ttggtcggcg tatcgctgct cttttcggtg ctcgtcggca ttccgctcgg
     2821 cgtgaccgcg gtgcgctttc acgcggccgg ccaggcaatc ttgttatcga gcgcggtagt
     2881 gcagacgata ccgtcgctgg cgctgttatg ttttctcatt ccggtgttcg gcgtcggcac
     2941 caagcccgcg ttggcggcgc tgtgcctgta cagcctgctg ccggtggtgc tcaacacctt
     3001 caccgggatt cgggccgtga atcccatcca tgcggagaat gcgcgggcgt tcggtttgaa
     3061 tcgcaggcag gtgctgttcc gcgtggtgtt gccgctcgcg agcccgacgc tgctcgcggg
     3121 aatcaaaacc gccaccatcg tcagcatcgg caccgcgacc ttggcggcat tggtgggggc
     3181 gggcggctac ggggcgccga tcgtgtcggg gctcgcgctc aacgatgtac ccaccattct
     3241 gaccggcgct attcccgcgg cgctcatggc gttggtggcc catggcatct tcgaactctt
     3301 aggactcgtg ctcattcccg cggggctgcg ccggcggtcg taaacggacg cggaacccgg
     3361 aacctaggcc ccccccgcac ccggaagcgg ccgaccaaaa tcgaaaaatc accccaaaac
     3421 gcggccaaat cctcgttttg agctatagta atgttaataa gaatgattcg tgttagcgtt
     3481 actacctggg tcatcgagtt gggatcgagc accatgccgc aaagccaagc cgcgtcggag
     3541 ggagtgtcga acttcgccgc cgtaccggga ttgaaccgct tcaccgccgc aggagtggcc
     3601 gtcaccgctc ttctgtcgtc cgcccgcgcg gacgtcgatg cggcggccgg cgatcagccc
     3661 gccttgccga gcgtcgaggt gaacggcacc gcgattcagt tcgagcgcga catatcgcag
     3721 tcgatcaaca cggtggataa aaaggagctg agcgagcaga accttaccct cgtgtccgat
     3781 gcgctgcgca atgtgccggg aatcaccctg aattcgggcg agggcggcgc acacggcgat
     3841 tcggtgaatc tgcgcggcct gtcgattccg gacagtttct tcatggatgg ggtgcgcgac
     3901 atcggccaat accagcgcga caccttcaac tcggatgcga tatcggtgct gctcgggccg
     3961 gcctcggcgg tgttcggccg cggctcgacc tcgggcgtga tcaacggcat cagcaagcag
     4021 ccgttgttgg cgccgctggc gagtttgagc gtgtcggccg gtcaggcgca cctgtggcgc
     4081 gagaccggcg atttcaatct gccgttgagc agcacctcgg cgctgcgtct cgccgtgatg
     4141 gatcagcgca acggcgtcga gcagcgcgat gaggttctgt accgccgtta cggcgttgcg
     4201 ccgacgctcg ccttcggcat cgagacggcg acgcgactga cgttgagtta tttcaaagaa
     4261 gaggaaaaca acatcccgga ttacggcatt ccgttcatcg acggtgcgcc ggccaacgtc
     4321 gctcgcagca actattacgg tctcttgaat tacgatcgca cgcgcaccaa cagcaacatc
     4381 ggcacgctgc gcttcgagca tgatttcagc gactcgatca cgttcagcga cagcttgcgc
     4441 tacgccaact acggcttcga gtacctggtg tccggcccct tcctgggcaa cgactccgta
     4501 gcgccgccgc cgccagacac
//
LOCUS       DASWZT010000171         3740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_44812, whole genome shotgun sequence.
ACCESSION   DASWZT010000171 DASWZT010000000
VERSION     DASWZT010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44812"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VGE92_04590"
     CDS             complement(<1..270)
                     /locus_tag="VGE92_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313128.1"
                     /translation="MLENSQSWVVLKFGGTSVSSAANWHNIAGIIRARIAASFRPVIV
                     HSALSGITDRLEALLTAAVAGTHQDALQQIEATHRDLARSLGIDPG"
     gene            complement(363..644)
                     /locus_tag="VGE92_04595"
     CDS             complement(363..644)
                     /locus_tag="VGE92_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313129.1"
                     /translation="MRSFYDWGLIDPIDFKGFSQPMSEREDEHFDIDDEFLGEAEESH
                     DFDPLLERTERRPKVAAAGKRGKAAWSRVEDVLAERQLEKELRDIFEDD"
     gene            complement(722..2422)
                     /gene="leuA"
                     /locus_tag="VGE92_04600"
     CDS             complement(722..2422)
                     /gene="leuA"
                     /locus_tag="VGE92_04600"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019836831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY1313130.1"
                     /translation="MMLRNPSSKYRAFTPVELSNRTWPSRVIAAPPTWCSVDLRDGNQ
                     ALIEPMDGARKRRMFDMLVKIGFKEIEVGFPAASQTDFDFVREIIEQGLVPDDVTIQV
                     LTQARPELIARTYESLKGARRAIMHVYNSTSAAQRRVVFRLDRAGITDIAVRGAQSVR
                     EHALRNPDTEWVFQYSPESFTGTELDFAVEICDAVNAVWEPTPQHRSILNLPATVEMA
                     TPNVYADQIEWFLRNVAHRDSLILSVHPHNDRGTAVAAAELAVMAGAERIEGTLFGNG
                     ERTGNVDIVTLALNLYSQGVDPKLDFSNINEAARCAEGCNQLPIHPRHPYVGDLVFTA
                     FSGSHQDAIKKGLAARSDADFWDVPYLPIDPSDLGRSYDSIIRVNSQSGKGGVAYLLE
                     RDYQLVMPRRLQIEFSQVVQATADITGKELTSQELWELFTREYLTPRTPFVYRSHQLA
                     ASTDGADSERITVQVENDGRLETWHGQGSGPIEAMVKAIALPFDVLSYEEHSRGQGSA
                     AKAVSYIEITTRSRRTLFGAGMHPNIITASLLAIFSAVNRAVAQQAMPAKAEAARTGT
                     "
     gene            2639..>3740
                     /locus_tag="VGE92_04605"
     CDS             2639..>3740
                     /locus_tag="VGE92_04605"
                     /inference="COORDINATES: protein motif:HMM:NF036723.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY1313131.1"
                     /translation="MHETVFNQTPDLSDFNLFSSDVVLQESVRREGAAAAVPELVRAG
                     AALGTAQTFEDARLANRHTPVLHTVNVRGERIDSIEFHPSWHALMRGIVARGYHTGPW
                     VLSAGRAVPGAHVARAAGYLLQAQVESGTLCPTTMTYGAVAALRRDAWLNQHWVPRLL
                     TREYDPRDIPIEVKQGGLIGMGMTEKQGGSDVRSNITRASRCDDGSYRITGHKWFFSA
                     PQCDAHLVLAQAQRGLSCFFVPRRLPDGSRNGIFLQRLKDKLGNRSNASSEVEFVDAW
                     GHRVGEEGRGIPTILEMATYTRLDCVTGSAGMMRQAVVQAVHHARHRKVFGAPLINQP
                     LMQSVLADLAIESEAATVLAMRLARAFDSDADS"
BASE COUNT          711 a         1161 c         1144 g          724 t
ORIGIN      
        1 gcccgggtca atgccgagcg agcgcgccag gtcgcggtgc gtcgcctcga tttgctgcag
       61 agcgtcctga tgtgtccccg ccacggccgc cgtcaacagc gcttcgaggc gatcggtgat
      121 gccggataac gcagagtgaa caatcaccgg ccggaaagag gcggcgatgc gcgcgcgaat
      181 tatgcccgcg atgttgtgcc aattggcggc ggaggacacg ctggtgccgc cgaacttgag
      241 cacgacccag gactgactat tttcgagcac ggcggtgatc gcacattgat ttcaattccg
      301 cgtcaaccgc gcatctcgct cgctgcgcgc caacatgcgc gaatgagcgc cgccaggcgc
      361 ggctaatcgt cttcgaaaat atcgcgcagt tctttttcta actggcgctc ggcgagcacg
      421 tcttccactc ggctccaagc cgccttgccg cgtttgccgg cagcggcgac tttagggcgc
      481 cgttcggtgc gctccaacag gggatcgaag tcgtgcgatt cttcggcttc cccgagaaac
      541 tcatcgtcga tatcaaaatg ttcgtcttcg cgttcgctca ttggttgaga aaagcccttg
      601 aaatcaatag gatcgatcag cccccaatcg tagaagctgc gcactatagc cgattctgcc
      661 cggcattcaa ctagcacgcc aaaaacaccc tgtccaggcg cgaattggcg aggacccctc
      721 tttaggtgcc ggtgcgcgcc gcctcggcct tggccggcat cgcttgttgg gcgacggcgc
      781 ggtttaccgc cgaaaaaatc gccaacaacg acgcagtgat gatattgggg tgcatacccg
      841 cgccaaacaa ggtgcgccgc gatcgggtgg tgatctcgat ataggacacc gccttggcag
      901 cgctgccctg cccccgagag tgctcttcgt aggacagcac gtcgaagggc agcgcaatgg
      961 ccttgaccat ggcctcgatc ggtccggagc cctgaccgtg ccaggtctcg agccgcccgt
     1021 cattctcgac ttgcacggtg attcgctcgc tgtccgcgcc gtccgtcgag gccgcaagct
     1081 ggtgcgagcg gtacacgaaa ggagttcgcg gagtcaggta ctcgcgagtg aaaagctccc
     1141 ataactcttg tgaagtcaat tccttacccg ttatatctgc agtagcttgg acgacctgac
     1201 tgaactcgat ttgcaggcgt ctgggcatga ccagttggta gtcgcgttcc aacaaataag
     1261 ccaccccgcc ctttccggat tgactgttga cccggatgat cgagtcatag gagcggccca
     1321 aatccgacgg gtcgatgggc agatacggta cgtcccagaa atccgcgtcg ctgcgcgcgg
     1381 cgagaccttt cttgattgca tcctggtgcg accctgagaa ggctgtgaac accagatcac
     1441 cgacgtacgg atgacggggg tgaatgggca gctgattgca accctcggca cagcgggcgg
     1501 cctcgtttat gtttgagaaa tccagtttcg gatcaacacc ttgtgagtaa aggttaagag
     1561 ccaacgtcac gatatccaca ttgccggttc gctcgccgtt gccgaacaag gtgccctcga
     1621 tacgctccgc gccggccatg acagcgagtt ccgccgcagc caccgcggtg ccgcgatcat
     1681 tgtgcggatg cacgctcaga atcagggagt cgcggtgcgc gacattgcgc agaaaccatt
     1741 cgatttgatc ggcatacaca ttgggcgtcg ccatctcgac cgtcgccggc aggttcaaaa
     1801 tcgatcgatg ctgcggagtc ggttcccaca ccgcattgac cgcatcgcat atctcgacgg
     1861 cgaaatcgag ttccgtgccg gtgaaactct ccgggctgta ttggaacacc cactcggtgt
     1921 ccgggttacg caaagcgtgc tcgcgcacgc tttgcgcgcc gcgcacggca atatcggtaa
     1981 tgccggcgcg atccaaacga aacaccacgc gccgctgcgc cgccgaggtc gagttataca
     2041 cgtgcatgat ggcgcggcgc gcgcccttca aggattcgta ggtgcgggca atgagttcgg
     2101 gtcgcgcctg cgtcagcacc tggatggtga catcgtcggg caccaggccc tgctcgatga
     2161 tttcgcgcac gaaatcaaaa tccgtctgcg aggccgccgg aaatccgacc tcgatctcct
     2221 tgaagccgat ttttaccagc atgtcgaaca tgcgccgctt gcgggcgcca tccataggct
     2281 cgatcaacgc ctgattgccg tcgcgcaggt cgacgctgca ccacgtcggc ggcgcggcaa
     2341 tgacgcggct cggccatgtt ctattgctca attcaacggg agtgaaggca cggtacttgc
     2401 ttgacggatt acgcaacatg attttctctc gctcgcccta gggggtcgca actcttctat
     2461 tattccggtc ttgtaagcgc cgaccgatag cgcaatgtag gtggcgccta gcggcgcagc
     2521 agcgcaagcg agcccggcag cgtcgagccg cggggacgtg tggacttgcg ggcgagggtg
     2581 cgcattacgc ccgaatttag cattgctggc gcttctcgcg caatagtggg tattttccat
     2641 gcacgaaacc gttttcaacc agactcccga tctgagcgat ttcaacctct tctccagcga
     2701 cgtggttcta caggagtcgg tgcggcgtga aggcgccgca gccgccgtac ccgaactggt
     2761 cagggcgggc gcggcgctcg gcacggcgca gactttcgag gatgcgcgac tcgccaatcg
     2821 ccacacgccg gtgctacaca ccgtcaatgt tcgcggcgag cgcatcgatt ccatcgaatt
     2881 tcacccctcc tggcacgcct tgatgcgggg aatcgtggcg cggggctatc acaccggccc
     2941 gtgggtcctt agcgcagggc gggcggtgcc cggagcgcac gtcgcccgcg ccgccgggta
     3001 tctgctgcag gcgcaagtgg agagcggaac gctatgccct acgaccatga cgtatggcgc
     3061 cgtcgcggcg ctgcgccgcg acgcgtggct gaatcagcac tgggtgccgc ggcttctcac
     3121 ccgcgagtac gacccgcggg acatccccat cgaggtgaag cagggcggcc tgatcggcat
     3181 gggcatgacc gaaaaacagg gcggttccga tgtccgcagc aacatcacgc gcgcgtcgcg
     3241 ctgcgacgac ggctcctatc ggattaccgg ccacaagtgg tttttctcgg cgccgcaatg
     3301 cgatgcgcac ttggtgctgg cacaggcgca gcgcggcctt tcctgtttct tcgttccgcg
     3361 ccgcctaccc gatggcagcc gtaacggcat tttcttgcag cgcctgaaag acaagctcgg
     3421 caaccgctcg aacgcctcgt ccgaagtcga attcgtcgat gcctggggac atcgcgtcgg
     3481 cgaagaaggc agaggtattc ccaccatcct cgagatggcg acctacacgc ggctcgactg
     3541 cgtcaccgga tcggcgggca tgatgcgcca ggcggtcgtg caagccgtgc atcatgcccg
     3601 ccatcgcaag gtattcggcg cgccgttgat caatcaacct ctgatgcaga gtgtgcttgc
     3661 ggatctggca atcgaatcgg aggcagcgac cgtgttggcg atgcgcctgg cacgtgcgtt
     3721 cgacagcgat gcggacagcg
//
LOCUS       DASWZT010000172         3127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_59328, whole genome shotgun sequence.
ACCESSION   DASWZT010000172 DASWZT010000000
VERSION     DASWZT010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59328"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VGE92_04610"
     CDS             <1..433
                     /locus_tag="VGE92_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY1313132.1"
                     /translation="VRTLQLDGRITNSQLAESVSLSESATLRRVRALEESGFIEGYTA
                     RINQQKAGCPVNVFVNITLERQEQAHLHAFEEAARKVPEVMECYLMSGEYDYLLRVVL
                     ADTADFERLHSQHLTRLPNVARVQSSFALRTVQKSRELPVR"
     gene            complement(459..1034)
                     /locus_tag="VGE92_04615"
     CDS             complement(459..1034)
                     /locus_tag="VGE92_04615"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY1313133.1"
                     /translation="MMARLSVSPALFAAAFLAAGIGVAAAATSYSADPQQSRLEFVGV
                     QAGADFKGAFHKFTAAVEFAPDALPSAHLDVLIDTNSVDSMDKDRDGTIRSSDILDVG
                     HFPTAHYVTRSITKTAAGYTASGALTLRGVTKDVPINFQFTPSAAGAKLEGSAQLKRL
                     DFGAGQGDWKSTEWVADAVKISFSLVLKPKT"
     gene            complement(1031..1603)
                     /locus_tag="VGE92_04620"
     CDS             complement(1031..1603)
                     /locus_tag="VGE92_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEY1313134.1"
                     /translation="MPSQGSSGRYTAVAQAFHWVIAALIVTQFVLGHIAVDLPNGMHK
                     LALFARHKSFGMTVLMLAILRLLWRLKNPPPELPAGMSAPERMIARATHAAFYVLLFA
                     MPLTGWMMSSAKNYAVSWFGLFTWPNLIGKNEAAFDALRATHDILSDVLFAIAVLHIL
                     AALKHHFWNKDDVLVRMLPFGSRPKSEKSL"
     gene            1662..2369
                     /locus_tag="VGE92_04625"
     CDS             1662..2369
                     /locus_tag="VGE92_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313135.1"
                     /translation="MFKSVGIAALALLLVACGVPRPRPGPQPPSAPAVAPNAPPAQRN
                     YLIDADRSELRILVYRAGPLARFGHNHVLVNRAIRGTVMAGAAGSAVISLQIPVAAFA
                     VDDSQARREEGADFEGDIPEDAKAGTVHNMLSAALLDADKNPLISISGVIGPIAAPDA
                     PVTVSAAISVAGHESKIAVPVTLQRDPAGVAASGILELRQTALGLTPYSLMLGALQVQ
                     DAMTLKFHIIATPATAG"
     gene            complement(2383..3105)
                     /locus_tag="VGE92_04630"
     CDS             complement(2383..3105)
                     /locus_tag="VGE92_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313136.1"
                     /translation="MLGRFLEYSLATPDIRESLEFYVKLGFSPAEVGEAWAHPYAVVT
                     DGRIYLGLHQEPIHAPSLTFVKPGLLKHAEVLEQMGIEFEFRRLGSNVFNELGWFDPS
                     GELVRLIEARTFSPSKRLDTNTSKCGYFLEIALPTPSREAATAYWEQFGFVGIEETDD
                     RLPHVSCTSDYLDLGLYDPAHLRRPMLRFEVDDVGGTLARLAETGISPAGEIPRPLRP
                     LPAAVLTAPEGTSILLSEGTAR"
BASE COUNT          626 a          960 c          973 g          568 t
ORIGIN      
        1 cgtgcgcaca ctgcagctcg atggacgcat taccaacagt caactggccg agagcgtcag
       61 cttgtcagaa tcagcgacgc tcagacgcgt ccgcgcattg gaggaatcgg gtttcatcga
      121 ggggtatacg gcgaggatca atcaacagaa ggcgggttgc cccgtgaatg tgttcgtcaa
      181 catcacgttg gagcggcagg aacaggccca cttgcacgcc ttcgaagagg ctgcgcgcaa
      241 ggttcccgag gtgatggagt gctacctcat gtcgggcgaa tacgactatc tgctgcgggt
      301 cgtgctcgcc gacaccgcgg atttcgagcg cttgcacagt cagcacctca cgcgcctgcc
      361 gaatgtcgcg cgcgttcagt cgagcttcgc gttgcgcacg gtgcagaaat cgcgcgagct
      421 tccggttcgt tagagcagcg cgcgaagcgg gcacgcggtt aggtcttggg cttgagcacc
      481 agcgaaaagc ttattttcac cgcatcagcc acccactcgg tgctcttcca gtcgccttgc
      541 ccggcgccga aatcgaggcg cttgagctgg gcactgcctt cgagcttcgc gcccgctgcg
      601 gacggtgtaa attgaaaatt gatcggtacg tctttggtga ccccgcgcaa ggtcaatgca
      661 ccgctcgccg tatagcccgc ggcggtcttg gtgatgctgc gagtgacgta gtgcgcagtc
      721 ggaaagtggc cgacatccaa gatatcggac gaacgtatcg tcccatcgcg atccttgtcc
      781 atcgagtcca ccgaattcgt gtcgatcagg acatcgaggt gcgcgctcgg cagtgcgtcc
      841 ggggcgaact cgacagcggc ggtgaactta tgaaacgccc ccttgaaatc tgcgcccgcc
      901 tgcacgccga cgaattccag gcggctttgc tgtggatccg cggaatagct cgtcgcggca
      961 gccgcaacgc cgataccggc cgctagaaac gccgcggcga acaacgccgg cgataccgaa
     1021 agtcgcgcca tcatagtgat ttctccgatt tgggcctgga gccgaagggc agcatccgca
     1081 ccagcacatc atccttgttc caaaagtgat gtttgagcgc ggcgagaatg tgcagcaccg
     1141 caatcgcgaa tagaacgtcg ctcaggatgt cgtgggttgc gcgcaatgcg tcgaacgccg
     1201 cctcattctt gccgatgaga ttcggccagg tgaacaatcc gaaccaactc accgcgtagt
     1261 tcttggcgga cgacatcatc cagcccgtca gcggcatggc gaacagcagc acgtagaatg
     1321 ccgcatgagt cgcgcgcgcg atcatccgct cgggcgcaga catgcccgcc ggcaattccg
     1381 gcgggggatt cttcagtcgc cacagcagac gcaatatcgc gagcatcagc accgtcatgc
     1441 cgaagctctt gtggcgcgcg aacaaagcga gcttatgcat cccgttgggc aaatccacgg
     1501 caatgtggcc gagcacgaac tgggtgacga tcaacgcggc tatgacccag tgaaatgcct
     1561 gcgcgaccgc ggtgtagcgt cccgacgacc cttgacttgg catgctgctg gctccgcgtg
     1621 caaactgttc gagctagtct agcaagacat cggggccctg catgttcaaa agcgtcggca
     1681 ttgcggcact cgcgttgctg ctggttgcgt gcggcgtgcc gcggccgcgg cctgggccac
     1741 aacccccgag cgcgcccgcg gtagcgccga atgcgccgcc agcgcagcgc aactacctaa
     1801 tcgatgcgga ccgatccgaa cttagaatac tggtgtaccg ggccgggccg ctggcgcgct
     1861 tcgggcacaa tcatgtactg gtcaatcgcg cgatccgcgg gacggtcatg gccggcgcgg
     1921 cgggttcggc cgtaatctcg ctgcaaatac ccgtcgccgc attcgcggtg gatgacagcc
     1981 aagcgcgccg cgaagaagga gccgatttcg agggcgacat tcccgaggat gccaaggccg
     2041 gaactgtgca caatatgctg agcgccgcgc tgctcgatgc cgacaagaat ccgctgatca
     2101 gcatcagcgg cgtcatcggc ccgattgccg cgccggatgc cccggtgacg gtgagcgccg
     2161 cgatcagcgt ggcgggacac gaatcgaaaa tcgccgtgcc cgtcaccctg cagagggacc
     2221 cggctggtgt ggcggcgagc ggaattctgg aattgcgtca aaccgcgctc ggcctcacgc
     2281 cgtatagctt gatgctcggc gcgctccagg tccaagatgc catgacgctc aagttccaca
     2341 tcatcgcaac gccggcaact gcgggttgag ctcgcgctaa cgctaacggg cagtaccctc
     2401 ggacagcaag atcgaggttc cttcgggcgc agtcaggacg gctgcgggta gcggccgcag
     2461 cggccggggt atttctccgg ccggcgagat gccggtctcc gcgagacggg ccaaggttcc
     2521 tccgacatcg tccacttcga agcgcagcat gggacggcgc agatgcgcgg ggtcgtaaag
     2581 tcccaggtcg agatagtcgc tggtgcacga tacgtgcggc aagcgatcgt cggtttcttc
     2641 aatcccgaca aaaccgaatt gttcccagta tgcggttgcc gcctcgcggc tcggggtcgg
     2701 cagcgcaatt tccagaaaat agccgcattt cgaggtattg gtatcgagtc gtttgctggg
     2761 cgaaaaagtc cgcgcctcga tcagccgcac cagctccccg ctgggatcga accagccgag
     2821 ttcgttgaac acattgctgc cgagacgacg gaactcgaat tcgattccca tctgttcgag
     2881 gacttccgcg tgcttgagta aaccgggttt gacgaaagtc agcgacggcg cgtggatcgg
     2941 ttcctgatgc agaccgaggt aaattcggcc gtcggtgacc accgcatagg gatgtgccca
     3001 cgcctctccc acctcggccg gggagaaacc gagcttgacg tagaattcta gagattctcg
     3061 aatatccggc gtcgcaagcg agtactccag gaaccggccg agcacaggtc tgtcaactca
     3121 ctccacc
//
LOCUS       DASWZT010000173         6089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_20594, whole genome shotgun sequence.
ACCESSION   DASWZT010000173 DASWZT010000000
VERSION     DASWZT010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20594"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..2346)
                     /locus_tag="VGE92_04635"
     CDS             complement(37..2346)
                     /locus_tag="VGE92_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008302352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEY1313137.1"
                     /translation="MKGDDLKEARKKAALDYHEFPTPGKLSITATKQLLNQSDLALAY
                     SPGVAAACEAIVEDPLNVFRFTARGNLVGVITNGTAVLGLGAIGPLAAKPVMEGKAVL
                     FKKFAGIDVFDIEVEQRDPMKLVELIAALEPTFGAVNLEDIKAPECFIVERELRRRMK
                     IPVFHDDQHGTAIIVAAACLNGLQVVGKDIRTIKMVVSGAGAAALACVDLLLDLGLAR
                     QNVWLTDLAGVVYEGRQELMDPDKARFAQTTQARTLNEVIGGADVFLGLSAGGVLTRG
                     MVEKMAARPLILALANPTPEILPEEVRAVRSDAVIATGRSDYPNQVNNVLCFPFIFRG
                     ALDVGATTITRQMEIASVHAIAELARQEQSDIVAAAYGGNEDLAFGPEYLIPKPFDPR
                     LIVKIAPAVAKAAMESGVATRPIADLDAYGQRLLQFVYHSGTLMQPLFVAARAVPAEH
                     SRIVYAEGEDERVLRAVQIVVDEKLATPILIGRPAVIARGIKRHGLRIRNAEHFTIVD
                     PEQDVRYREYWQEYYRLAQRSGVTAQYAKLELRRRTTLIAAMLLKKGEADGLICGTIS
                     TTARHLRYIDQVIGRRAGVNVYGAMSGLILPGRQVFIVDTHVNLDPSALQLAEITQLA
                     ANQLCAFGLTPKVALLSHSNFGSSDAPSAVKMRETLGLLHARMPDLEVDGEMHGDCAL
                     EESARKAILPDSTLHGSANLLVCPNLDSANIAYNLLKSAAGHNVAVGPILLGCAAPAH
                     ILTPAATVRRIVNMTAVTVVEASGAFARH"
     gene            2476..3399
                     /locus_tag="VGE92_04640"
     CDS             2476..3399
                     /locus_tag="VGE92_04640"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY1313138.1"
                     /translation="MRDLVAELNRDGFALIRGFLSPSEAAGIGAEAHRMYLEGLKHHA
                     TYRHKNLLFEILDDPKANRRVVLQAHWTAWLSPLLEQMRRSPKYFAVLEPFLGPDIKQ
                     ITHQIHWKPPGAKYTSYRFHQDARFREGNVKDFNFLKSSVTTGLAIDRQTAENGALRV
                     FPGSHRLGYLGISDDGPLMVGQTQDEELRRTGLDPSSVVTCAMEPGDLLLWTLFTVHG
                     SAPNVSHYDRRFMINSYVRASDSERGEWAFRGGVSTALGSEPQICKYEQLHERPGPFY
                     VESDWTEEANSAALRSAAAASDLARPSEASK"
     gene            complement(3300..4742)
                     /locus_tag="VGE92_04645"
     CDS             complement(3300..4742)
                     /locus_tag="VGE92_04645"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1313139.1"
                     /translation="MPVTAAYKRLVVALLTAAYTCNAMDRGVISIIGQAMKVDLHLSD
                     TQLGLLSGTAFALLYAFGGIPLARLAERVNRVNIITLALAVWSALSALCGAASSFPQL
                     LVIRVGVGVAEAGCSPPAHSLISDYFAPSQRASALSIYSCGISLGYLAAAVGGGYLAQ
                     RWGWRTACAAVGVPGILIAVLIKYWVREPPRVTLSSAPSFSFGAEWRELRAVGRALLC
                     DRPVLHMILGVTAGGFAAYGFYAFLPAYFSRAYGLGYGLTGVIAGLAGGVAVGLGILA
                     GGFIADGFAKRDLRWYAWVPGIGGVIATPLYWLALLPADWRVAAGLLAAAGFFQYASL
                     GPTFGVVQNVVDRRRRATATAFLYICLNVFALGGGPLFTGWVIDRFADADLVQPQTGT
                     AMPFAAGAVFKTACPGGAAAAGANTASQSICHAALVRATRQGLEVTLLFFVWACAHYL
                     LASLGLAKSLAAAALRSAAEFASSVQSDST"
     gene            complement(4754..5305)
                     /locus_tag="VGE92_04650"
     CDS             complement(4754..5305)
                     /locus_tag="VGE92_04650"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY1313140.1"
                     /translation="MTFHPDFLLEVATACGRNAHFRAAAIPPGKFSAGLSGQMRRLTL
                     GLWDGEEIAFELAAAALQIGCSAKQKVRVSVRDRQRVASIVRHLEDAFCEPCSLTSLA
                     QRVQLSPYYFLRMFKRVTGQSPAQYVINVRLRAAADELLGTQAAIADIAFNTGFNDLS
                     HFNSSFRAVFGRSPRRWRLGVRP"
     gene            complement(5718..>6089)
                     /locus_tag="VGE92_04655"
     CDS             complement(5718..>6089)
                     /locus_tag="VGE92_04655"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313141.1"
                     /translation="TAGQVNDYLREAMGEDFTAKDFRTWGATLHAITLLARAPLPAAA
                     SERALKLEIAAVVKQVAAKLRNTPAVCRKSYINPAVFESWRSGLIHKVFNGSLSLAAP
                     RKAETLVLAFLRHEAQASSPD"
BASE COUNT         1207 a         1928 c         1886 g         1068 t
ORIGIN      
        1 cggttacccg tgtggaccca aattcccgac tcaagttcaa tgcctcgcaa atgccccgct
       61 ggcttccacc accgtgaccg ccgtcatgtt gacgatgcgc cgcaccgtgg cggccggagt
      121 caagatgtgc gcgggggcgg cacaaccgag caggatgggg cccacggcca cattgtggcc
      181 cgccgcagac ttgagcagat tataagcgat gttggcgctg tcgagattgg ggcacaccaa
      241 gagatttgcg ctgccatgca gcgtgctgtc cggcagtatc gccttgcggg ccgactcctc
      301 cagtgcgcaa tcgccgtgca tttcgccgtc cacttccaaa tccggcatgc gggcatgcag
      361 caggcctaag gtttcgcgca tcttcaccgc ggagggcgca tcgctgctgc cgaaatttga
      421 atgcgacagc agtgcgacct tgggcgtgag accgaaggcg cacagctgat tcgcggccag
      481 ctgggttatt tcagccaact gtaaagcgct cgggtcgaga ttgacgtgcg tgtccacgat
      541 gaaaacctgc cgtcccggca ggatcagacc gctcatagcc ccatagacgt tgacgcccgc
      601 gcgccgccca atgacctggt cgatgtaacg caagtgccgc gccgtggtac tgatggttcc
      661 gcatatcaag ccgtccgcct cgcccttctt cagcagcatg gcggctatca gggtggtacg
      721 ccggcgcaac tcgagcttgg cgtattgtgc ggtgacaccg ctgcgctggg cgagacggta
      781 gtactcctgc caatactcgc gatatcggac atcctgctcg ggatcgacga tggtgaagtg
      841 ctcggcgttg cgaatgcgca gtccgtggcg cttgatgccg cgagcgatga ccgccggccg
      901 accgatcagg atcggcgtcg ccaacttctc gtcgacaact atctgcacgg cgcgcagcac
      961 ccgctcatct tcgccctccg cgtaaacgat gcggctgtgt tcggcaggca cggcgcgtgc
     1021 ggcgacgaat aggggctgca tcagcgtgcc gctgtggtac acgaactgca acaggcgttg
     1081 gccgtaagcg tccaagtcgg cgatcggtcg tgtcgccacg cccgattcca tggcggcttt
     1141 cgcgactgcg ggagcaatct tgacgatgag gcgcgggtca aagggtttgg gaatgaggta
     1201 ttcggggccg aaagccaggt cctcattgcc gccgtaagcg gcggcgacaa tgtcgctttg
     1261 ctcctggcgc gccaactcgg cgatcgcgtg aacgctggct atttccatct gccgagtaat
     1321 ggtggtggcg cccacgtcga gcgcaccgcg aaagatgaaa ggaaagcaaa ggacgttatt
     1381 cacctggttc ggatagtcgc tgcggccggt ggcgatcacc gcgtcactgc gcaccgcgcg
     1441 cacttcctcc ggcaggattt ccggggtggg atttgcgagc gcaaggatca atggccgcgc
     1501 cgccatcttc tcgaccatgc cgcgagtcaa gacgccgccg gcggacaagc cgaggaacac
     1561 gtccgcgccg ccgatcacct cattcaaggt ccgggcttgc gtggtctgcg cgaagcgtgc
     1621 cttgtcggga tccatgagct cctggcggcc ctcatagacg acgccggcga gatcggtcaa
     1681 ccaaacattc tgccgcgcca accccaaatc cagcagcagg tccacgcagg ccagcgccgc
     1741 cgccccggcg ccgctgacca ccattttaat cgtccggatg tccttgccga ccacctgcag
     1801 gccattgagg caggcggcgg ccacgatgat cgcggtgccg tgctggtcgt catggaacac
     1861 cggtattttc atgcggcggc gcaattcgcg ctccactatg aaacattccg gggccttgat
     1921 gtcctcgaga ttgacagcgc cgaacgtcgg ctcgagagcg gcaatcagct ccaccaattt
     1981 catcgggtcg cgttgctcga cctcgatgtc gaagacgtcg atcccggcga atttcttgaa
     2041 caacaccgcc ttgccctcca tcaccggttt ggcggcgagc gggccgattg ccccgagacc
     2101 gagcaccgca gtgccgttcg tgatgactcc caccaaattg ccgcgtgcgg taaagcggaa
     2161 tacattgagc gggtcctcga cgatggcttc gcacgccgcg gcgacgcccg gagaataggc
     2221 gagagccagg tcgctctggt tgagcagttg cttggtggcg gtgatcgaca gctttcccgg
     2281 ggtcgggaac tcgtggtagt cgagcgccgc cttcttgcga gcttccttga gatcatcgcc
     2341 tttcatctga tttctccgcg agaaccggcg gccttcagta agtggcgtgg gccgatctgc
     2401 agtgaaatca tacggcaagg ccatggtgaa agggactaaa ctcggctctg ggcgaactgc
     2461 tgggtaggag ggctcatgcg tgatttggtc gccgagctaa atcgcgacgg gttcgcgctg
     2521 atccgcggct ttctatcgcc gtcggaagcg gcaggcatcg gagcggaagc gcaccgcatg
     2581 tacctcgagg ggctgaaaca tcacgcgacc tatcggcaca agaatctgct gttcgagatt
     2641 cttgatgacc cgaaggcgaa tcgtcgcgtg gttctgcagg cacactggac cgcctggctg
     2701 agtccgctgc tcgagcaaat gcggcgaagc ccgaagtatt ttgccgtgct cgaacccttt
     2761 ctggggccgg acatcaagca gatcacacat caaatccatt ggaagccgcc gggcgcgaaa
     2821 tatacctcgt atcgatttca ccaagatgcg cgttttcgcg agggcaacgt caaggatttc
     2881 aatttcctga agagctccgt cacgaccggt ctcgcgattg atcgacaaac agccgagaac
     2941 ggcgccctgc gagttttccc cggaagccat cggctcggct atctcgggat ctcggacgat
     3001 ggaccgctca tggttggtca gacccaggat gaggaattac gacgcaccgg gctcgacccg
     3061 tcgagcgtcg taacctgcgc catggaaccg ggcgatctgc ttctctggac gctgtttacc
     3121 gtgcatggat cggctccgaa tgtcagtcat tacgatcggc gattcatgat caacagttac
     3181 gtccgggcga gcgacagcga acgcggcgaa tgggcctttc gcggcggcgt ttcgactgcg
     3241 ctcggttccg aaccgcaaat ctgcaagtac gagcagctgc acgaacgtcc ggggccgttt
     3301 tatgtcgagt ccgactggac agaggaagcg aactcagcgg cgctgcgcag tgccgcggct
     3361 gccagcgact tggcaaggcc gagcgaggcg agcaaataat gcgcgcacgc ccagacgaaa
     3421 aacagcaacg tcacctcgag cccttgccgc gtcgcgcgca ccaaggccgc gtggcagatc
     3481 gattggcttg cggtgttcgc tcccgccgcg gccgccccgc cgggacatgc ggtcttgaag
     3541 acggcgccgg ccgcaaatgg catcgcggtg ccggtctgcg gctgaaccag gtcggcgtcg
     3601 gcaaagcggt cgatcaccca gcccgtgaat aacggtccac cgcccaacgc aaagacattg
     3661 agacagatgt atagaaacgc agtcgccgtt gcccgccgcc gccgatcgac cacattttgc
     3721 accacgccaa acgtgggtcc gagcgaggcg tactgaaaaa agccggccgc cgcgagcagg
     3781 ccggccgcca cacgccaatc cgccggcaac agcgcaagcc aatacagcgg cgtagcgatg
     3841 actccaccta tacccggaac ccaggcgtac cagcgcaggt cgcgcttggc gaagccatcg
     3901 gcgatgaacc cgccggcgag tattcccaag cccaccgcca cgcctccggc gaggccggcg
     3961 atcacacccg tgaggccgta gccaaggccg taagcacggc taaaataagc tggtaggaac
     4021 gcgtagaaac cataggcggc aaatccgccc gcggtgacgc caagaatcat gtggagcacg
     4081 ggccggtcgc ataagagcgc acggccgacg gctcgcaact cgcgccattc ggcaccgaac
     4141 gagaaagacg gcgcgctcga tagcgttacg cgcggcggtt cgcgcaccca gtatttgatc
     4201 agcactgcaa ttaggattcc cggcacaccc accgcagcac atgccgtgcg ccaaccccag
     4261 cgctgcgcga ggtagccgcc gcctaccgca gcggccaaat agccgagcga tatcccgcat
     4321 gagtaaatgg acagcgccga ggcgcgttgc gacggcgcaa agtaatccga gatcaacgag
     4381 tgcgcgggcg gcgagcagcc ggcctcggca accccgacac cgaccctgat gacaagcagc
     4441 tgcggaaaac tcgacgccgc gccgcataag gcgctcaagg ccgaccacac cgccagggct
     4501 agcgtgatga tattgacgcg attgacgcgt tcggcgagtc gcgccagagg aattcccccg
     4561 aatgcgtaca ggagcgcgaa ggctgttccg ctgagcagtc ccaattgcgt gtcgctcagg
     4621 tgcaggtcga ctttcatcgc ttgaccgatg atcgagatca cgccgcgatc catcgcgttg
     4681 caggtgtatg ccgccgtcaa cagcgcgacc acgaggcgct tgtacgccgc cgtgaccggc
     4741 atcggctcgg cagctacggt cgcactccca gccgccagcg gcgcggactg cggccaaaga
     4801 ccgcacggaa gctcgagttg aagtgcgata ggtcgttgaa tccggtgttg aaggcaatgt
     4861 ccgcgatagc cgcttgcgtg ccgagcaatt catccgcagc cgcgcgcagc cgcacattga
     4921 tcacatattg cgccgggctc tgtcccgtaa cgcgcttgaa catccgcaag aaataatatg
     4981 ggctcaattg cacccgctgc gcgagactgg tgagcgaaca tggctcgcag aacgcatcct
     5041 cgaggtggcg cacgatcgat gccacgcgtt gccgatcacg cacggacacc cggacctttt
     5101 gcttcgccga acaaccgatt tgcagtgcgg ccgcggccaa ttcgaaggcg atttcctcgc
     5161 catcccacaa acccaaggtc aggcgccgca tttggccgga tagaccggcg gagaatttgc
     5221 ccggcgggat ggcagcggcc cgaaaatgcg cgtttctgcc gcaagctgtc gctacttcca
     5281 agagaaaatc gggatggaac gtcacgacgc ggcgccgatt gccgagccgg actgtgcccg
     5341 gtaatcgatg cagcccgaca gcacaacgcc caaagcgggc acagtgtatt gcctttcctg
     5401 cggtcgactc ctcgaggagg gtaggcaaac ttcttccgtg accgcaaagc gtgcagcagg
     5461 catgcgcgca ttctgaatcg caatcgcagc gccgacaatc caaatgagca atattcccaa
     5521 gccccggccg cgaatcatcc gtatgctttg cgcgaactcg tcgtcctggt gcacgttccg
     5581 agcctgtggg cctcgccacc gccagggtgg gcaccgtcgg cgagaatcca gctgacggcc
     5641 ctgtgttggg ccgcggtact ggcgggatct gcctggatag tcgcgcgctc gatgcgcaat
     5701 cctcgagtaa cgtcgggcta gtcggggctc gaggcctgcg cctcatggcg caggaacgcc
     5761 agcaccagcg tttccgcctt gcgcggcgcg gccaggctca agctgccgtt gaacacctta
     5821 tgaatcaggc cggagcgcca agactcgaac accgcggggt tgatatagga cttgcggcaa
     5881 actgccggcg tgttgcgcag tttcgcggcg acttgtttca ccaccgctgc gatttccagc
     5941 ttcaaggcac gttcgctcgc cgctgcgggc aacggcgcgc gggcgagcaa ggtgatggca
     6001 tgcaaggtgg ctccccacgt acggaagtcc ttggccgtga aatcctcccc catcgcttcg
     6061 cgcagatagt cgtttacttg accggctgt
//
LOCUS       DASWZT010000174         1714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_15762, whole genome shotgun sequence.
ACCESSION   DASWZT010000174 DASWZT010000000
VERSION     DASWZT010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15762"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VGE92_04660"
     CDS             <1..809
                     /locus_tag="VGE92_04660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1313142.1"
                     /translation="MRSSNKTFYTGFIRDLTERQSTEARLQELQSELVHISRLTAMGE
                     MASTLAHELNQPLSSIANYLKGGMRLLQDRTDEESRMTRDALDKATEQAMRAGQIIRR
                     LRDFVSRGESERRAENMSKLVEEASALALVGVRDVHIRFDLDPAAEVLADKVQIQQVL
                     INLMRNAMEAMAASERRDLTVKSTLIADDMVEVRVADTGPGIAPEIAGHLFQPFFTTK
                     IHGMGVGLSICRTIIEAHEGRISVEPNPDGGTVFCFTLRAMTKQDADSDA"
     gene            790..1428
                     /gene="fixJ"
                     /locus_tag="VGE92_04665"
     CDS             790..1428
                     /gene="fixJ"
                     /locus_tag="VGE92_04665"
                     /inference="COORDINATES: protein motif:HMM:NF006900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator FixJ"
                     /protein_id="HEY1313143.1"
                     /translation="MQTAMPDTQTVYIVDDDDAARQSLEFLLKSHKLTVQAYESAIAF
                     LEALPNISTGCVITDVRMPGMSGIELLKRLKSATHPVPVIIITGHGDVPLAVEAMKYG
                     AADFLEKPFDDELLLGAVRSALNRLGKDGKREAERAEVLDKLATLSTRERQVLEGLVA
                     GNPNKVIAFDLSISPRTVEIYRANVMSKMAAASLSDLVRKALIAGILDNTPA"
     gene            1501..>1714
                     /locus_tag="VGE92_04670"
     CDS             1501..>1714
                     /locus_tag="VGE92_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEY1313144.1"
                     /translation="MEKQRQPGFISILARLWRRAPATPEGSAASGVSLGEIAGLAPEE
                     ALRRLQGSAEGLSPDEAEARLRTMGPN"
BASE COUNT          356 a          537 c          518 g          303 t
ORIGIN      
        1 aaatgcgctc gtccaacaag acattttaca ccggcttcat ccgcgaccta accgagcgcc
       61 aaagcacgga agcgcgcttg caggaactgc aatccgaatt ggttcacatc tcccgattga
      121 ccgcgatggg tgagatggcg tccacgctcg ctcatgaact caaccagccg ctgtcgtcga
      181 tcgccaatta tctcaagggc ggcatgcgcc tgcttcagga ccggacggac gaagaatcgc
      241 gcatgacgcg agatgcgctc gacaaagcca ccgagcaggc gatgcgcgcc ggtcagatca
      301 ttcgccgcct gcgcgatttc gtgtcgcgcg gcgaaagcga gcgccgcgcg gaaaacatga
      361 gcaagctcgt ggaagaagcg agcgccctcg ccttggtcgg cgtcagggac gttcacattc
      421 gcttcgatct cgatccggcc gctgaggtct tggccgacaa ggtccagatc cagcaggtcc
      481 tgatcaattt gatgcgcaac gccatggagg cgatggccgc atctgaacgg cgagacttga
      541 ccgtaaagtc cacgctcatc gccgacgaca tggtcgaggt gcgcgttgcc gacaccgggc
      601 ccggcatcgc gccggaaatt gccggccatc tttttcaacc gttcttcacg accaagattc
      661 acggcatggg cgtcggcctt tcgatctgcc gcaccatcat cgaggcgcac gagggccgca
      721 tatcggtcga acccaacccg gacgggggta cggttttctg tttcactctg cgggcgatga
      781 ccaaacagga tgcagacagc gatgcctgac acccagactg tttatatcgt cgatgatgac
      841 gacgcggcgc ggcagtcgct ggagttcctg ctgaagagcc acaagctcac ggtacaggct
      901 tacgaatccg ctatcgcatt tctcgaggcc ctgccgaaca tcagtaccgg ttgcgtgatt
      961 accgacgttc gcatgccggg aatgagcggc atcgaattgc tcaagcgcct taagagcgcg
     1021 acccacccag ttccggtcat catcataacc gggcatggcg acgtcccgct tgcggttgaa
     1081 gccatgaagt acggcgcggc ggattttctg gaaaagccgt tcgacgacga actgctgctc
     1141 ggcgccgttc gttcggcctt gaaccgcctg ggaaaggacg gcaagcgcga agccgagcgc
     1201 gcggaagtcc tcgacaagct cgccacgctt tcgacccgcg agcggcaggt gcttgaaggc
     1261 ctggtggcgg gcaatcccaa caaagtgatc gccttcgatc tgtcgatcag cccgcgaacc
     1321 gtcgaaattt accgtgccaa tgtcatgagc aagatggcgg cggccagcct gtccgacctt
     1381 gtgcgaaaag ccctgatcgc gggcattttg gacaacacgc ccgcatagtc acaatatggt
     1441 accccccggc gccgacagcc tgggcgattt tcatttaacg ccaacgatga aagcaagagc
     1501 gtggagaagc aacgccagcc aggcttcata tccatccttg ctcgtttatg gcgccgggcg
     1561 ccggccacgc ccgaaggctc ggccgcgagc ggcgtgagcc tcggtgagat tgccggcctc
     1621 gcgccggagg aagcgctgcg ccgcctgcag gggagcgcgg agggtctctc gcccgacgaa
     1681 gccgaggctc ggctgcgcac gatgggtccc aacc
//
LOCUS       DASWZT010000175         3954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_73877, whole genome shotgun sequence.
ACCESSION   DASWZT010000175 DASWZT010000000
VERSION     DASWZT010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73877"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1644)
                     /locus_tag="VGE92_04675"
     CDS             complement(<1..1644)
                     /locus_tag="VGE92_04675"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEY1313145.1"
                     /translation="MYAKTRFLLTAAKRWRVGFAASLLMPLLAHADMPAYPSVTTERL
                     VNAQKDNGWLMYRRNYESTGYAPFDQINGATVGKLAVAFDYTSDLSQGHEAAPVVNGK
                     YMFVTTPMDHLIAMDAASGKVLWKYDYPLDKKALKTVCCDVVNRGVALYGDLVYMGTL
                     GNHIVALDAATGAVKWDTALAPPGVGYFISGAPLVVKGKVIIGDGGGEYGSRGFIVGI
                     NAETGKVEWKTYTTAAPGTPGGDTWPAGAYKTGGGNPWITGTYDNASNTLFWGVGNPG
                     PWLATLRPGDNLYTDSVLALNPDNGKIKWHYQWTPNDTWDYDGANETVMTDLNYGGKA
                     YKALVHADRNGWFYALDRSNGKLIYAEPFVHTTSITGMKDGVPQSDPAMRPTIDKQVF
                     TCPSFLGGKNWWPISVDPQTQMAYIPTQHACMDIKGAEPVAYKAGLAFLDESFEVKHD
                     PTTDNWGSVQAVDLNTGKQTWQHETHLPWSGATLATSGGLMFSGSTDGHFLAFDAKTG
                     KVLWTSPQLSSGIIGVPTTYMVDGKQYVAVWAGWGGAGPI"
     gene            2121..>3954
                     /locus_tag="VGE92_04680"
     CDS             2121..>3954
                     /locus_tag="VGE92_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEY1313146.1"
                     /translation="MAASMSVEAAGPHADRVLRVAGHDMAVVTAGANVTTTVDVSWRR
                     CLNEFKLDPARDYQPTVLDLCRLNDLRAEHEDLVQIARAEMDSLYEQIAGSGYALLLA
                     DTDGVILCEKVDPLLKKMFVRAGLIVGAQWSEQSQGTNGIGTCVAEARPITIHESDHF
                     RARHVGLSCSAAPIHDSNGRVIAVLDASSVGAGGTRESKMHTVALVNTSARLIEKCLF
                     LRRHSAVSMLRFHHRPEFVDLLHDGAIAVAADGTIVATDSTGLRLLGTEDRKSLVGCS
                     IADVFDTTYEELLAAAGAGRRTVQELRDNRFGRRYYASLVYAGRFGGSAPLASVTPRA
                     VHEVVPAARGDLTLPMTLADLAGEDPQMLRNLRNAQRIADTNVSVVIRGPTGSGKEAF
                     AKALHLASSRANRPFVAVNCAAIPESLIESELFGYSRGAFTGARREGMRGRIAQACGG
                     TLFLDEIGDMPLLLQTRLLRVLEELEVTPLGAETAIKVDIRVICASHQNLSEMIACGD
                     FREDLYYRLNGISLELPPLAARQDKEALICKCIARESAGGPAACAETNALRRLLAYNW
                     PGNIRELRNTIRTALAVCDNRVIRLDDLPAEIRRYQPRLRDADDE"
BASE COUNT          727 a         1204 c         1250 g          773 t
ORIGIN      
        1 tatcgggccc gcgccgcccc aacctgccca cacggcgaca tactgcttac cgtcgaccat
       61 gtacgtggtg ggcacgccga tgatgccgga actgagctgc gggctcgtcc acaggacttt
      121 gcccgtcttg gcgtcgaacg ctaagaagtg cccgtccgtg ctgccgctga acatcaagcc
      181 tcccgaggtt gcgagtgtgg cgccggacca gggcaaatga gtttcgtgct gccaggtctg
      241 tttgccggta ttcaagtcca cggcctgcac cgaaccccag ttgtcggtgg tcggatcgtg
      301 cttgacctcg aaggattcat cgagaaaggc gagccccgcc ttgtaggcga ccggctccgc
      361 tcccttgatg tccatgcagg cgtgttgcgt gggaatatag gccatctgag tctgcggatc
      421 gacagaaatc ggccaccagt tcttgccgcc gaggaagctc gggcaggtga acacctgctt
      481 gtcgatcgtc ggccgcatcg ccggatcgga ctgcggcacg ccgtccttca taccggtgat
      541 ggacgtggtg tgcacgaacg gctcggcata gatcagtttg ccgttgctgc gatcgagcgc
      601 atagaaccaa ccgttgcgat cggcatgtac cagcgcctta taggcctttc ccccgtagtt
      661 aagatcggtc atgaccgtct cattggcgcc gtcatagtcc caggtgtcgt tcggcgtcca
      721 ctggtagtgc cacttgatct tgccgttgtc gggattcagc gccaacaccg aatcggtgta
      781 caagttatcg cccggacgca gcgtcgcgag ccacggaccg ggattgccca caccccaaaa
      841 gagcgtattg ctggcattgt cgtacgtgcc ggtgatccag ggattgccgc ccccggtctt
      901 gtaggcaccc gccggccagg tgtcgccgcc cggcgttcca ggcgcagccg tggtgtaggt
      961 tttccattcg accttgccgg tctcggcgtt gatgccgacg atgaaaccgc gcgacccgta
     1021 ctcgccgccg ccatcgccga tgatcacctt gcctttgacc accagcggcg caccggaaat
     1081 gaaatagccg acgcccggag gcgctagagc cgtgtcccat ttcaccgcgc cggtcgcggc
     1141 atcgagagcg acaatgtgat tgcccagagt gcccatgtag acgagatcgc cgtaaagcgc
     1201 cacgccacgg ttgacgacgt cgcagcaaac ggtcttcaag gctttcttgt cgagcggata
     1261 gtcgtacttc cacagcacct tgccgctggc cgcgtccatc gcgatcagat ggtccatcgg
     1321 cgtggtgaca aacatgtact tgccgttgac caccggagcc gcctcgtgcc cctgcgacaa
     1381 atcactggtg taatcgaacg cgaccgccag cttgccgacg gtggcgccat tgatttgatc
     1441 gaacggcgca tagcccgtgc tttcgtagtt gcgccgatac atcagccagc cattgtcctt
     1501 ttgggcgttc accagccgct cggtcgtcac cgacgggtac gccggcatgt ccgcatgggc
     1561 cagcagtggc atcagcagcg atgcggcgaa gccgacgcgc cagcgcttgg ccgcggtcaa
     1621 aagaaaacgt gtctttgcat acatctagaa ttccccttgt ttaccctcgt tatatcaaac
     1681 cccatgcgct gggcgccgat cgcgccaacg gaccgcggat cagcgcgatc cgtcggctgc
     1741 cgctagtgcg tctcgcggcc ggtgcggtaa accgcgcagc ccgtatcaag gggaagtgca
     1801 aacgccatgc catgagcacc atggcacgag tcagtgaatt tcgcagtaag gggaaatacg
     1861 cgatgcgcta cgcacaagtc caccaccctg ggggataccg ctgcgccaca tcgagccggc
     1921 atactgctag tgacgcgcag cgtgggcggg tggttgtccg gaagaatcca cttgcgcggc
     1981 gaggagtgtg gccttcgcgt gtcatattcg tgacagattt cagctgccgc ttgacacact
     2041 cgggacgtgg acgttagtta agagaaaaaa gtcggggggg ggcagatggc ttgtactcag
     2101 agcgttgtat tgtcgcgctg gtggctgcgt ccatgagcgt cgaggcggca ggaccgcacg
     2161 ccgatcgcgt gctgcgcgtc gcggggcatg acatggctgt cgttaccgcc ggagcgaatg
     2221 tcacgacgac ggtcgacgtc tcctggcggc gttgcttgaa tgaattcaaa ctcgatccgg
     2281 cgcgcgatta ccaacccacc gtgctcgatt tgtgccgcct gaacgatcta cgtgccgagc
     2341 acgaagatct ggtgcagatt gcccgcgctg aaatggattc tctctacgaa cagatcgccg
     2401 gttccggtta tgcgctgctg ctcgccgaca ccgacggggt cattctctgt gagaaagtcg
     2461 atccgttact gaagaagatg ttcgttcggg caggactgat cgttggcgcg cagtggagcg
     2521 aacagtcgca gggcaccaac ggaatcggga cctgcgtcgc cgaagcgcgg cccatcacca
     2581 ttcacgaatc cgaccacttt cgtgcgcgcc acgtcggcct gtcctgttcg gcggcgccga
     2641 ttcacgattc gaacggtcgc gtcatcgcgg tcctggacgc atccagcgtg ggcgcgggcg
     2701 gcacgcgcga gtcgaagatg cacaccgtcg cgctggtcaa tacctcggca cgattgatcg
     2761 agaagtgttt gttcttgcgc cgccacagtg cggtctcgat gttgcgcttt catcatcgac
     2821 ctgaattcgt cgatttgttg catgatggtg cgatagccgt ggcggccgac ggaaccatcg
     2881 tcgccaccga ctcgaccggg ctgcggttgc tggggacaga ggatcgaaag tcgttggtgg
     2941 gatgctcgat tgccgacgtc ttcgacacga cgtacgaaga actgttggca gcggccggag
     3001 ccggccggcg caccgtgcag gagttgcgcg acaaccgctt cggccggcgc tattacgcga
     3061 gtttggtcta tgcgggccgg ttcggcggga gcgcgccgct tgcaagtgtg acgccgcggg
     3121 cggtgcacga ggtggtgccc gcagcacgcg gcgatctcac actgccgatg acgctcgcgg
     3181 atcttgcggg cgaggacccg cagatgctgc gcaatctgcg taatgcgcag cgcatcgccg
     3241 acaccaacgt gtccgtcgtg attcgaggtc caacgggatc cggcaaggag gcattcgcaa
     3301 aagcgctgca cctggccagc agccgggcga accggccgtt tgtcgccgtc aattgtgcgg
     3361 cgatccccga atcattgatc gaaagcgaat tgttcggcta ctcgcgcggc gcgttcacgg
     3421 gcgcgcgccg cgaagggatg cgcggccgta tcgcccaagc gtgcggcgga acgctattct
     3481 tggacgaaat tggcgatatg ccgctcctgc tgcagacgcg cttgctgcgg gttcttgaag
     3541 aactggaagt cacgccgctc ggcgccgaaa cggccatcaa ggtggacatc cgtgtgattt
     3601 gcgcttcgca ccaaaacttg agcgagatga ttgcctgcgg cgattttcgc gaggatctct
     3661 attatcgatt gaacggcatc agcctggagc ttccgccgct ggccgcgcgt caggacaagg
     3721 aagcgctgat ttgcaagtgc atagcgcgcg agtccgccgg gggccctgcg gcgtgcgccg
     3781 aaacgaacgc gctgcggcgg ctgctcgcgt acaattggcc aggcaatatt cgcgaactgc
     3841 gcaatacgat tcgcacggcg ctcgccgtgt gcgacaaccg cgtcatccgg ctggatgatt
     3901 tgccggccga gatacgacgc taccagccgc gcttgcgcga tgcggatgat gaag
//
LOCUS       DASWZT010000176         2397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_54569, whole genome shotgun sequence.
ACCESSION   DASWZT010000176 DASWZT010000000
VERSION     DASWZT010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54569"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="VGE92_04685"
     CDS             <1..800
                     /locus_tag="VGE92_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313147.1"
                     /translation="LAVLAAGDRQALRPLPVSSLILISPAIRARGLPSGLVITPRVMS
                     GVSATTGAFRWLERHEDLDFAKYESFPLNAWFQLVLLDERVAAATRGTLGVPLLGFVS
                     WDDATTDAAASLKFFRRAADERSRLSITARDSEVPAVTQLAADARISVRGAAVAERGV
                     VSYSHLSFGIPEANTHYGLAGDYANCVWYPVKGGRGSAKYCNCLTAAMHDSACPDHEP
                     ASGDALRYGEANPTDQRQYVLRRLTFNPRFDTDVRAIGDFIDEASRR"
     gene            830..2374
                     /locus_tag="VGE92_04690"
     CDS             830..2374
                     /locus_tag="VGE92_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313148.1"
                     /translation="MMSSIDRGNSLFIIALTAVLGWLAAAVVPVSAHHSQTAAPLTVG
                     LGVTHFANSGSPRAQRDFLDGLLLLHSFEYPAARGAFQSAERTDPSFAMAYWGEALTY
                     NQTLWGEQDLEAARAALAKLASTPAQRAAKAGTARERAYLAAVEQLYGNGDKTERDAK
                     FSAAMGSLARAYPNDLDARAFYALSILGLTNGVRNVANYMRAAAEAEEVYEKDRHHPG
                     ALHYLIHAYDDPIHAPLGLRAARLYGKVAPAASHAQHMPSHIFFALGMWDEAIAANLA
                     SLKIARAQNDGGYHSLVWLTYAYLQEGRRAEAQQLIRSVAHDVGTAPTKDNRIRLALV
                     RAIWLVETRGAEGADALEAVDSRGIASIGYFDTYDFARGLTAAADVNEARAALERLQA
                     RIDGAGGASRAVSADWHDTVTATELAQAAIMATALDGTITYYAGDRRAGIARIRAAIA
                     AADREEFEYGPPWSVKPLDELLGELLLADGRRQEAAAAFEKTLAVYPNRRLAREGLAA
                     TQVTNE"
BASE COUNT          418 a          754 c          781 g          444 t
ORIGIN      
        1 cgctcgcggt gctcgctgcg ggtgacaggc aagccctgcg accgctgccg gttagcagcc
       61 tgattctcat ttcaccggcg atacgtgcgc gcggcctgcc gtctggcctg gtcatcacgc
      121 cgcgggtgat gagcggcgtc agcgcgacga ccggcgcatt tcgctggctg gaacggcacg
      181 aggatctgga cttcgcgaaa tacgagtcgt ttccgttgaa cgcgtggttt cagttggttt
      241 tgctcgatga gcgggtggcg gctgcaacgc gaggcacgct cggcgtgccg ctgctggggt
      301 tcgtgagttg ggacgacgcc accacggacg ccgccgcttc cctgaagttc tttcgtcgcg
      361 ccgccgatga acgcagccgt ctgtccatca ccgcccgaga ttccgaggtg ccggcggtca
      421 cccaactcgc ggctgacgca cgcatcagcg tgcgcggcgc tgccgtggcc gagcggggcg
      481 tcgtcagcta ctcgcacctc tccttcggca ttccagaagc gaacacgcac tatggcctcg
      541 ccggagatta tgcgaactgc gtctggtatc cggtcaaggg cggcagaggc tctgccaagt
      601 actgtaactg cctcaccgcg gcgatgcacg attccgcgtg ccccgaccac gaacctgcaa
      661 gtggcgatgc gttgcgctat ggcgaagcga atccgaccga tcagcgtcag tatgtgctgc
      721 gcagactcac gttcaatccg cgcttcgaca ccgatgtgcg ggcgattggc gatttcatcg
      781 acgaagcctc tcgccgctga cgcttgagcc attttttgga gagtgttgaa tgatgagctc
      841 gatcgatcgc ggcaacagcc tgttcattat tgcactgacc gccgtgctcg gctggttggc
      901 tgcggccgtg gtcccggtat ctgcccatca ctcgcagacc gctgcgccgc tcaccgtcgg
      961 cttgggtgta acccactttg cgaattcagg gtcgccgcgc gcgcagcgcg actttctcga
     1021 cggattattg ttgctgcaca gctttgagta cccggcggcg cgcggcgcct tccagtccgc
     1081 cgagcgcacc gacccgagtt tcgccatggc ctactggggc gaggcgctca cctacaatca
     1141 aacactctgg ggagagcagg acctcgaggc cgcgcgcgcc gcgctcgcca agctcgcgtc
     1201 cactcccgcg cagcgagcgg ctaaggccgg gacggctcgc gagcgcgctt acttggcggc
     1261 ggtggagcag ctctacggca atggcgacaa gacggagcgc gatgcaaaat ttagtgcggc
     1321 gatgggctcg cttgcccgag cgtatccgaa tgatctcgac gcccgggcct tctatgcgct
     1381 gtccatccta ggtttgacca acggcgtgcg caatgtcgcc aactacatgc gtgcggcggc
     1441 cgaggccgaa gaggtatacg aaaaggaccg acatcatcca ggcgcgctgc actacctgat
     1501 tcacgcctac gatgatccca tccatgcacc gctcggacta cgcgccgcgc gcctgtacgg
     1561 caaggtcgcg cccgcggcct cgcacgcgca gcacatgccc tcgcacatct tcttcgctct
     1621 gggaatgtgg gatgaggcga ttgcggccaa tcttgcttca ctgaagattg cccgcgcaca
     1681 gaatgacggc ggatatcact cgctggtgtg gttgacgtac gcgtacctgc aggagggtcg
     1741 gcgcgcggag gcgcagcagc tgatacggtc tgtcgcccac gatgtcggga cagcgcctac
     1801 caaggacaat cgtatcaggc tcgcgctcgt gcgcgccatc tggctggtgg aaacccgtgg
     1861 cgccgaaggt gccgatgcgc ttgaggcggt tgatagccgc ggcatcgcct cgatcggcta
     1921 tttcgatacg tacgattttg cgcgtggatt gaccgcggcc gcggatgtga acgaagcgcg
     1981 cgcggcgctc gagcgattgc aggcgcgaat agatggcgcg ggtggcgcat cgcgggcagt
     2041 gtcggccgat tggcacgaca cggtgacggc gactgagctc gcacaggcgg caatcatggc
     2101 gaccgcgctc gatggcacaa tcacttacta tgcaggcgat cgaagggccg gaattgcgcg
     2161 gattcgcgcg gctattgcgg cggccgatcg cgaagaattc gagtacggcc ccccctggtc
     2221 ggtaaaacca ctcgatgaat tactcggtga gcttttgctg gcggatggcc ggcgccagga
     2281 ggcggccgcc gcattcgaaa aaacgctcgc cgtctatccg aatcgacgcc ttgcccgtga
     2341 agggctggcc gcaacccagg tgacgaatga atagaaaatg gccgcgcagc tccaggg
//
LOCUS       DASWZT010000177         3476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_64202, whole genome shotgun sequence.
ACCESSION   DASWZT010000177 DASWZT010000000
VERSION     DASWZT010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64202"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /gene="cysS"
                     /locus_tag="VGE92_04695"
     CDS             complement(<1..1068)
                     /gene="cysS"
                     /locus_tag="VGE92_04695"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY1313149.1"
                     /translation="MELRLYDTLTREKRPFAPIDAGRVRMYVCGPTVYDFAHIGNARP
                     VIVFDVLFRLLRHIYGDRHVTYVRNVTDVDDKINARAAERGISIQELTKATYDNFKSD
                     VEALGCLSPTVEPRATEHIEQMKTLIERLVKFGNAYVAEDNILFNVPSMPDYGQLSKR
                     PLDEMIAGARVEVAPYKKDPTDFVLWKPSKPGEPAWVSPAGVKAPGRPGWHIECSAMA
                     WKHLGETFDIHGGGIDLVFPHHENEIAQSRCAFHTGVMANYWMHNGFLQVEGEKMSKS
                     LGNFVTIRELENVYPGDVLRLLMLTTHYRQPIDWTREGLELARKELLVWAELVLDYFD
                     SKENLEKVVADLDQKLIDALLD"
     gene            1466..1699
                     /locus_tag="VGE92_04700"
     CDS             1466..1699
                     /locus_tag="VGE92_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313150.1"
                     /translation="MFIIANDGDGYGIDRCLASGDRCGAAAANAYCKGHEFASAMSFR
                     KVDRDEITGAIPTGGSGGCNGSNCDVVAIVCTR"
     gene            complement(1776..2174)
                     /locus_tag="VGE92_04705"
     CDS             complement(1776..2174)
                     /locus_tag="VGE92_04705"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY1313151.1"
                     /translation="MLKLDQHNDLVTLVARLFFASLFVTYGYFKITAFAGTAAYMAKQ
                     GLPLPPLAAAIAVLVELGGGLLILLGYQTRLVALICAAYVVIAALIAHRNFADGNQMS
                     HFFKNMAITGGFLALMVSGGGRYSLDARKS"
     gene            2380..3015
                     /gene="maiA"
                     /locus_tag="VGE92_04710"
     CDS             2380..3015
                     /gene="maiA"
                     /locus_tag="VGE92_04710"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_026437509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEY1313152.1"
                     /translation="MKLFTYFRSSAAYRVRIALNLKGLPYETASIHLTKDGGQQRQSA
                     FRAVNPQMRVPALELSGGEVLTQSLAIIEYLDEINTEPPLLPADALARAHARAIAQMV
                     ACDIHPLNNLVALQYLKRQLKHEQPEIDAWYHHWVIEGFTAIEAMIEPGPYVCGAHVT
                     LADICLVPQVFNARRLKVPLDRFPKIVAADAACMKLSAFDKARPENQPDAE"
     gene            3086..>3476
                     /locus_tag="VGE92_04715"
     CDS             3086..>3476
                     /locus_tag="VGE92_04715"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY1313153.1"
                     /translation="MITHKLARVVLWMVGALLSFSVMAVSIRELANGGLSIFEILAIR
                     SGVALVVLFFLLAVRPALRVHALPRRMGLNLLRNTVHYASQYAWALSLTTLPLATVFA
                     LEFTMPGWTALLAVWLLHEKMTPSRMGV"
BASE COUNT          654 a         1129 c         1015 g          678 t
ORIGIN      
        1 gtccaacaaa gcatcgatta acttttggtc gaggtcggca acaaccttct ccaaattctc
       61 ttttgaatca aaatagtcca acactagctc cgcccagacc aatagttcct ttctagcgag
      121 ttcgaggcct tcgcgtgtcc aatcgattgg ctgtctatag tgagtggtca acatcaaaag
      181 acgaagaacg tctccgggat aaacgttttc caactcccta atcgtcacga aattgccgag
      241 actcttgctc atcttctcgc cttccacctg caagaagccg ttgtgcatcc agtaattcgc
      301 catcacgccg gtatggaagg cgcagcgcga ttgcgcgatc tcgttctcgt gatgcggaaa
      361 caccaggtcg atgcctccgc cgtggatgtc gaaggtctcg ccgagatgct tccatgccat
      421 ggccgagcac tcgatgtgcc agcccggccg ccccggcgcc ttgacgccgg ccggcgagac
      481 ccaagcgggt tcgcccggct ttgacggctt ccacagcacg aaatcagtcg ggtctttctt
      541 gtaaggcgcg acctcgacgc gagcgcccgc gatcatctcg tcgagcggac gcttcgagag
      601 ctggccgtag tccggcatcg acggcacgtt gaatagaata ttgtcctcgg ccacgtaagc
      661 gttgccgaat ttcaccagcc gctcgatcag cgtcttcatc tgctcgatat gctcggtggc
      721 gcgcggctcg accgtcggcg acaggcagcc gagcgcttcg acgtccgact tgaaattgtc
      781 gtaagtggcc ttcgtcaatt cctgaatcga gataccgcgc tcggcagcgc gcgcattgat
      841 cttgtcgtcc acatcggtga cgttgcgcac gtaggtgacg tgcctatcgc cgtagatgtg
      901 gcgcagcagc cggaacagca cgtcgaacac gatgaccgga cgcgcgttgc cgatatgggc
      961 gaagtcgtag accgtcggcc cgcacacata catgcgcacg cgcccggcat cgatcggcgc
     1021 aaacggccgc ttctcgcgcg tcagcgtatc gtagagcctg agctccatcg ctcttcctct
     1081 tgcgccaccg tcgcggactg cgctcgacga cgcgggcgcg gcggtcctcg tgcctgctgg
     1141 cccgggcgtt gctttatctc gatggagaaa agacggccgc agccagcgtg gtgcgctagc
     1201 taataatctc gcggcaaatg ccgcagatat ggttaaggcc gttcatcaca ggcgcacaat
     1261 gatgcgttgt gccggaaccg tcaaggcccg caaactccag cgccgctagg gttaagcggc
     1321 ccttaacgaa tttgcccgca ttctcctcat cggtgccgga cggggcgcct ccaaccaagc
     1381 gggctttgtc atgcgtacac tcctggtcgg cctcgccgcc tttctctcca tctgtgtcgt
     1441 tggctccgcg caggcggaaa agcgcatgtt catcatcgcc aatgacggcg acggctacgg
     1501 catcgaccgc tgtctcgctt cgggcgaccg gtgcggcgcg gcggccgcca acgcctattg
     1561 caaaggccac gaattcgcgt cggcgatgtc ctttcgtaag gtcgaccgcg acgaaatcac
     1621 cggcgccatt ccgaccggcg gctcgggcgg ctgcaacggc agcaactgcg acgtcgtcgc
     1681 catcgtctgc acgcggtaat tcaagatttt aggaatgccg tgactagcgc ccaaggggcg
     1741 ctggttttcg tttcgcctgc tgccgggact tattgctagg acttgcgcgc gtccagcgaa
     1801 tagcgccccc cgccgctgac catcaacgcc aggaagccgc ccgtaatcgc catgtttttg
     1861 aagaagtgcg acatctgatt gccgtcggcg aaattgcgat gcgcgatcag cgccgcgatc
     1921 acgacatagg cagcgcaaat gagcgccacg agacgggtct gatagccgag tagaatgagc
     1981 agaccgccac cgagctcgac aagaaccgcg atcgcggcgg ccagcggcgg cagcggaagc
     2041 ccctgtttgg ccatgtaggc ggccgtgccg gcgaaagcgg tgatcttgaa atagccgtag
     2101 gtcacaaaca gcgacgcgaa aaatagccgt gcaaccagcg tcacgagatc gttatgttgg
     2161 tcgagtttga gcatcgcttc cccctgtttc ctgaaacgtc ttcagcgcca tctcgatcgg
     2221 acggctgtcc gcaaactaac acgaaggcaa agactgggaa ggagatttac tcctcgccgt
     2281 tgggatgcgt ccccaaggaa agcctgccca acggcggctc gccaaccggc tcgacccccg
     2341 ctagattgcc gtcatcaccg tcaagaccgg ggtccagccg tgaagctctt tacttatttc
     2401 cgctcgtccg ccgcctatcg cgtgcggatc gcgctcaatc tcaagggcct gccctacgaa
     2461 acggcgtcca tccatttgac caaagacggc ggccagcaac gccagtcggc atttcgcgcc
     2521 gtcaatccgc aaatgcgcgt gccggcgctc gaattgtccg gcggcgaggt tttgacccag
     2581 tcgctggcga tcatcgaata tctcgacgag attaataccg agccgccgct gttgccggcc
     2641 gacgcgctgg cgcgcgcgca cgcgcgcgcc attgcgcaaa tggtcgcctg cgacattcat
     2701 ccgctcaaca atctcgtcgc gctgcagtac ctcaagcgcc aactcaagca tgagcagccg
     2761 gagatcgacg cctggtatca ccactgggtg atcgagggct ttaccgcgat cgaggcgatg
     2821 atcgagcccg gtccctacgt ctgcggcgcg cacgtgacgc tggccgacat ctgcctcgtg
     2881 ccgcaggtgt tcaacgcgcg gcgccttaag gtgccgctcg accgctttcc gaaaatcgtc
     2941 gcggccgacg ccgcctgcat gaaactgtcg gcgttcgaca aagcgaggcc cgagaaccag
     3001 ccggatgccg agtgacggcc cgacgccggc ctgctagacc acggcccgcg cctcgcccac
     3061 ccctcagcag cagccgaatt agcccgtgat cacccacaaa ctcgcccgcg tcgtcctctg
     3121 gatggtcggc gcactgctct cgttttcggt catggcggtc tcgatccgcg aactggccaa
     3181 cggcggcttg agcattttcg aaattcttgc catccgcagc ggcgtcgctt tggtggttct
     3241 gttctttttg ctggcggtgc ggcccgcgct gcgggtgcac gcgctgccgc ggcgcatggg
     3301 attgaatctg ttgcgcaaca ccgtgcacta cgcctcgcaa tatgcttggg cgctaagcct
     3361 caccacgctc ccgctcgcga ccgtgttcgc gctcgaattc accatgccgg gctggaccgc
     3421 gctgctggcg gtctggctgc tgcacgagaa gatgacgccg agccgcatgg gcgtgg
//
LOCUS       DASWZT010000178         5370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_35153, whole genome shotgun sequence.
ACCESSION   DASWZT010000178 DASWZT010000000
VERSION     DASWZT010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35153"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VGE92_04720"
     CDS             complement(<1..225)
                     /locus_tag="VGE92_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017480625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY1313154.1"
                     /translation="MANYNTNEFKSGLKILIDGDPYAIVENEMVKPGKGQAFNRVRVR
                     NLKTGRTIERTWKSGDTVEAADVVDTDMQYL"
     gene            404..2467
                     /locus_tag="VGE92_04725"
     CDS             404..2467
                     /locus_tag="VGE92_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY1313155.1"
                     /translation="MSEQGAVPMIVMTRSQDHVEAINSTLRKAGHPVHCTWLPDARDL
                     GDALTQINPEMLIAFIEELGVELGSLMKVKQQNAPGMPVLIVREHVDEAAIADAMRLG
                     AQDVVTLANRSRLQSVATRELRAYRLERALSTTLSSAREYREQLQNFLEGSADAITHV
                     QEGIIVDANRAWLELFGYSGDDALSGTPLMDLFEQETHPALKGALVACLQGKWSGHGL
                     KVQALLSDGSNLPLELLLTKADYENEPAIRIAISAMHKKDHDLEVQLADAVKNDATTR
                     FLQQRYLIAAVRERCTLSVKGGVRQFAHIKPDRFIEIQHAIGVLASEDFMAQLAELLR
                     AQLTANDLCGRFGGNGFLILLERGTARDVETWAENVAKKVNEHVFAIEDKTLSATVTV
                     GLGLLPPTNPDVGAAISDAVSATRRGRELGGNQMYAVDKSDTDTRVQAYDKIWVKHIK
                     SALMENRFRLVQQPIASLLGEDKGMFDVLVRMLDEQGTEVLPAEFIAAAERNDLMKNI
                     DRWVIGASMSFAANRKATCIFVRISKDTVLDKSLIAWLDTQLKSLKIEAKRICIQVTE
                     DLANQYVRQTKELTENLRKLGIRFALEHFGTGRDPLKLLADLDMNFIKIDGSLMQGLS
                     TNQIQQQRVKGLVEAAKRKSIETVAERVEDANTMAVLWQLGVEFIQGYFVNAPEDVTM
                     AGDRH"
     gene            complement(2476..4728)
                     /gene="parC"
                     /locus_tag="VGE92_04730"
     CDS             complement(2476..4728)
                     /gene="parC"
                     /locus_tag="VGE92_04730"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEY1313156.1"
                     /translation="MKDQELNFDGIEQEPLKSFAERAYLDYSMYVILDRALPHLGDGL
                     KPVQRRIVYAMSELGLAAGAKHKKSARTVGDVIGKFHPHGDSACYEAMVHMAQDFAYR
                     YPIVDGQGNWGSTDDPKSFAAMRYTESRLTRYATLLLQELGASTVDWAPNFDGSLDEP
                     TMLPARVPNLLLNGGSGIAVGMATDIPPHNLREVVKACVALLDDPDLSTRKIMAHIKG
                     PDLPTGAEIVSPRDDLVQMYDTGSGSFKARATYKIEDGEIVIEALPFQVSGSKVQEQI
                     AAQMQQKKLPMVEDLRDESDHENPTRLVIVPKSNRVDTAALMAHLFATTDLERNYRVN
                     LNVIALDGRPRVMSLKELLLEWLQFRKSTVTRRLNHRLAKLSARLHLLDGLLIAYLNL
                     DAVIRIVRTEDQPKPVLIKRFKLSDEQAEAILETKLRHLARLEEMKIREEQKELSEER
                     DEIAGILKSKARLTRLVRDELLSDADEYGDERRTRLVEREAAQAIAETELLSSEPVTV
                     VLSRLGWVRAAKGHDIDVRSLSYKGGDEFQAAARGRNLQQAVFIDSTGRAYSLDAHQL
                     PAARGYGEPLSGTVDPPDGATFAAVLIGEPDEVWLLASDAGYGFAVKLRELYARNKKG
                     KSVLKVPENARVLPAQPLAQENALAIFVNNDGEVLALPRSHIEEMSAGKGQNLFGIPG
                     KKSADRDEFLVAIAVVGAGEVLTVYSGNSAPMRLKYDELKQFQDKKGQRRQRFSRAYK
                     QVDRLEVAPG"
     gene            complement(4725..>5370)
                     /locus_tag="VGE92_04735"
     CDS             complement(4725..>5370)
                     /locus_tag="VGE92_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019691486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEY1313157.1"
                     /translation="FLVEGDSAGGSAKQARDRVTQAIMPLRGKILNTWEADTGEVLQS
                     QEVHNISIAIGVDPAASDLSGLRYHKICILADADSDGQHIATLLCALFLRHYRPLVTA
                     GHVYVCMPPLFRIDAGKEVVYALDEAEREKALRRIAAENPRAKPVVTRFKGLGEMSPL
                     QLRETTMAPQTRRLVQMTIDARDSPDQMLDMLLAKKRASDRRAWLETKGNLARI"
BASE COUNT          982 a         1683 c         1650 g         1055 t
ORIGIN      
        1 caggtactgc atgtccgtgt cgacgacatc ggcggcttcc acggtgtctc cagatttcca
       61 ggtacgctcg atggtgcggc cggttttcag gttgcgcacc cgaacgcggt tgaaagcctg
      121 gcccttgccc ggtttgacca tctcgttctc gacgatcgcg taaggatcac cgtcgatcag
      181 gattttcaaa cccgacttga attcattcgt attgtagttc gccatcgcag gccttgagtc
      241 ttcggagggc gcgtatgata gccgcaacct cgagccttgg ccaattcgga cggaaaaagc
      301 ccgcttggcg gcctcgagtc ctgccacggg ctagacgtcg gtcccagatt cgttaaatcc
      361 caaggtgtac actttgggcc ccttattggg agaattttga cctgtgagcg aacaaggggc
      421 cgttccgatg atcgtcatga cgcggtcgca ggaccacgtc gaggcgatca acagcacgct
      481 gcgcaaggcc ggtcatccgg tgcattgcac gtggctgccg gacgcccgcg atctgggcga
      541 tgccctgacg caaatcaacc ccgagatgct gatcgcgttc atcgaagagc tgggcgtcga
      601 gttgggctcg ctcatgaagg tcaaacagca gaacgcgccc ggcatgccgg tgctgatcgt
      661 gcgcgaacac gtcgatgagg cggcgatcgc cgatgccatg cggctcggcg cgcaggatgt
      721 ggtgaccctc gcgaatcgca gccggttgca atcggttgcg acccgcgagc tgcgcgccta
      781 tcggctggag cgcgcgctct cgaccacgct gtcctccgcc cgcgagtatc gcgagcagct
      841 gcagaacttt ctcgaaggtt cggccgacgc catcacacat gtgcaggaag gcatcatcgt
      901 ggatgcgaat cgcgcatggc tcgagctgtt cggctattcg ggcgatgatg ccctcagcgg
      961 cacgccgctg atggatttat tcgagcagga gacgcatccg gcactcaagg gcgcgctggt
     1021 ggcgtgcctg cagggcaagt ggtccggcca cggactcaaa gtgcaggcgt tgctgtcgga
     1081 cggctcgaat ttgccgctcg agttgctgct caccaaggcg gactacgaaa acgaacccgc
     1141 gattcgcatt gcaatatccg cgatgcacaa gaaggatcac gatctcgaag tgcagctggc
     1201 cgatgccgtc aagaacgatg ccaccacgcg ctttctgcag cagcgctatt tgatcgccgc
     1261 ggtgcgcgag cgctgcacgc tgagcgtgaa gggcggcgtg cgtcaattcg cgcacatcaa
     1321 accggaccgt ttcatcgaaa tccagcatgc gatcggtgtg ctcgccagcg aggatttcat
     1381 ggctcagctc gccgaactgc tgcgcgcgca gttgaccgcc aatgacttgt gcggccgctt
     1441 cggcggcaat ggtttcttga ttctgttgga gcgcggtacc gcccgcgacg tcgagacgtg
     1501 ggccgagaac gtcgcgaaga aggtcaacga gcatgtgttc gcgatcgagg ataagaccct
     1561 ttcggcgacc gtgaccgtgg gcctgggact cttgccgccg accaatcccg atgtcggcgc
     1621 ggcgatcagc gatgcagtca gcgcgactcg ccgcggccgc gaactcggcg gcaatcaaat
     1681 gtacgccgtg gacaaatccg acaccgacac gcgcgtgcag gcgtacgaca agatttgggt
     1741 caagcacatc aagagcgcgc tgatggaaaa tcgcttccgc ctggtgcagc agccgatcgc
     1801 gagcttgctc ggcgaggaca agggcatgtt cgacgtgctg gtgcgcatgc tggacgaaca
     1861 gggaacggaa gtgctgccgg ccgaattcat cgcggccgcg gaacgcaatg acttgatgaa
     1921 gaacatcgat cgctgggtga tcggtgcttc gatgtcgttc gccgccaacc gcaaggcgac
     1981 ctgtatattc gtgcgcatct ccaaggatac ggtgctcgac aaaagcctga ttgcgtggct
     2041 cgacacccaa ctcaaatcgc tcaagatcga agcaaagcgg atttgcatcc aagtcacgga
     2101 ggacctcgcc aatcaatatg tgcgccagac caaggagctg acggaaaacc tgcgaaagct
     2161 cggcatccgc ttcgccttgg agcatttcgg taccggccgc gatcctttga aactgctcgc
     2221 cgatctcgac atgaatttca tcaagataga cggctcgttg atgcagggat tgtcgaccaa
     2281 tcagatccag cagcagcgcg tgaagggtct ggtcgaagcg gccaaacgca agagcatcga
     2341 gaccgtcgcc gaacgcgtcg aggatgcgaa caccatggcc gtgctgtggc agctcggagt
     2401 cgaattcatc cagggctact tcgtcaacgc cccggaggat gtcaccatgg cgggcgaccg
     2461 ccactagtcg cgcggctagc ccggcgccac ctccagccgg tcgacttgtt tgtaggcgcg
     2521 cgagaatctt tgccgacgct ggcccttctt gtcttgaaac tgtttgagct catcgtactt
     2581 gagcctcatc ggcgcactgt tgccgctgta gacagtcagc acttcgcccg cgccgaccac
     2641 ggcaatcgcc accagaaact catcgcgatc ggcggatttc ttgcccggga tgccgaataa
     2701 attctggccc ttgcccgcgc tcatctcttc gatgtgtgag cgcggcagcg ccagcacttc
     2761 gccgtcgttg ttgacgaata tcgccaacgc attctcctgc gccagcggct gcgccggcag
     2821 tacgcgcgcg ttctccggca ctttgaggac cgacttgccc ttcttgttgc gcgcatacag
     2881 ctcgcgcagc ttcacggcga atccatagcc cgcgtcgctt gcgagcagcc agacttcgtc
     2941 gggttcaccg atcaacacgg cggcgaatgt ggcgccgtcc ggaggatcga cggtgccgga
     3001 cagcggttcg ccgtaaccgc gcgccgccgg cagttgatgc gcatccaggc tgtaggcccg
     3061 gccggtggaa tcgatgaaca cggcctgttg caggttgcgg ccccgggcgg cggcctggaa
     3121 ctcatcgccg cccttgtagc tcaaggagcg gacgtcgata tcgtgccctt tcgccgcccg
     3181 cacccagccg agacgcgaca gcaccacggt caccggctcg ctgctgagaa gttcggtctc
     3241 cgcgatggcc tgcgccgctt cgcgttccac caatcgcgtg cggcgctcgt cgccgtactc
     3301 atcggcatcg ctcagcagtt catcgcggac caaccgcgtc aggcgcgcct tgctcttcaa
     3361 gatgccggcg atctcgtcgc gttcctcgct gagttccttc tgctcctcgc gaattttcat
     3421 ctcttcgagc cgcgcgagat ggcgcagctt ggtttcgagt atcgcctcgg cttgctcgtc
     3481 cgagagcttg aagcgtttta tgagcacggg cttaggttga tcttcggtgc gcacgatgcg
     3541 gatcaccgca tcgaggttca agtacgcgat caacagtcca tcgagtaggt gcaggcgcgc
     3601 gctgagcttg gccagccggt ggttgagccg ccgcgtgacc gtggacttgc ggaactgcag
     3661 ccattccagc agcagttcct tgagtgacat cacccgcggc cgcccgtcca aggcgatcac
     3721 gttgaggttc acccggtaat tgcgttccaa gtcggtggtg gcgaacaggt gcgccatcag
     3781 cgcggccgta tcgacgcggt tggacttcgg cacgatcacc agccgggtgg gattctcgtg
     3841 atcggattcg tcgcgcagat cctcgaccat cggcagcttt ttctgctgca tctgcgcagc
     3901 gatctgctcc tgcaccttgc tgcccgagac ctggaacggc agcgcctcga tcacgatctc
     3961 gccgtcctcg atcttatagg tcgcgcgcgc cttgaaactg ccggaaccgg tatcgtacat
     4021 ctgcaccaaa tcgtcgcgcg gcgagacgat ttccgcgccg gtcggcagat cgggtccctt
     4081 gatgtgcgcc atgattttgc gcgtgctcaa gtccggatcg tcgagcagcg ccacgcacgc
     4141 cttgaccact tcgcgcaaat tatgcggcgg tatgtcggtg gccatgccga cggcgattcc
     4201 ggagccgccg ttcaacagca gattcggcac gcgcgccggc agcatggttg gctcgtcgag
     4261 cgagccgtcg aaattcggcg cccaatccac ggtgctcgcg ccgagctcct gcagcagcag
     4321 cgtcgcgtag cgcgtcaggc gcgattcggt gtagcgcatg gcggcaaatg atttcggatc
     4381 gtccgtcgac ccccaattcc cctgcccgtc gacgatggga tagcggtacg cgaaatcctg
     4441 tgccatgtgc accatggcct cgtaacaggc tgaatcgccg tgcggatgga acttgccgat
     4501 tacatcgccg acggtacgcg cggacttctt gtgcttggcg ccggccgcca gacccagctc
     4561 gctcatcgca tagacgatgc gccgctgcac cggcttcagg ccgtctccca ggtgcggcaa
     4621 cgcccggtcc aaaatgacat acatcgagta atcgaggtag gcgcgttcgg caaacgactt
     4681 gagcggctcc tgctcgatgc cgtcgaagtt gagctcctga tccttcatat gcgagccaga
     4741 ttacccttgg tctcgagcca tgcacgccga tcggaggcgc gttttttcgc gagcagcata
     4801 tcgagcatct gatcggggct gtcgcgcgca tcgatggtca tctgcaccag tcggcgggtc
     4861 tgcggcgcca tggtggtttc gcgcagctgc agcgggctca tttcccccaa acccttgaag
     4921 cgcgtcacca ccggcttcgc gcgcggattc tcggcggcga tgcgccgcag cgctttctcg
     4981 cgctcggcct cgtcgagtgc gtagaccact tccttgccgg catcgatgcg gaacagcggc
     5041 ggcatgcaca cgtacacatg tccggccgtc accaacgggc ggtagtgccg cagaaacaga
     5101 gcgcacagca gcgtcgcgat atgctgccca tcgctgtcgg cgtccgccaa gatgcagatc
     5161 ttgtgatagc gcaagcccga caggtcgctc gccgcgggat cgacgccgat cgcgatgctg
     5221 atgttgtgca cttcttgaga ttgcaggact tcgccggtat ccgcttccca ggtgttgagg
     5281 attttgccgc gcagcggcat gatggcctga gtgacgcggt cgcgggcctg cttggcggag
     5341 ccgccggccg aatctccctc caccaagaac
//
LOCUS       DASWZT010000179         5117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_54576, whole genome shotgun sequence.
ACCESSION   DASWZT010000179 DASWZT010000000
VERSION     DASWZT010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54576"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VGE92_04740"
     CDS             <1..303
                     /locus_tag="VGE92_04740"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEY1313158.1"
                     /translation="HEERQFGHPAWQAGKKTFALARHDGRRLTLCFWVGVDQQGLLVA
                     DARYHVPPYMGHNGWIALEVGTDCDWSEIKALSLQSYRHFALKRMLDKLGPEWSKE"
     gene            436..1617
                     /gene="argE"
                     /locus_tag="VGE92_04745"
     CDS             436..1617
                     /gene="argE"
                     /locus_tag="VGE92_04745"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF005710.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HEY1313159.1"
                     /translation="MSTERPSAASRAMIERLIGFNTVSRDSNLGLIEWVRDYLRGFGA
                     TTRLTYDATGKKANLFATLGDSKKPGLILSGHTDVVPVDGQAWDTDPFLCSERDGKLF
                     ARGSADMKGFIGIILAQAPKFVAALNDNRLDAPLHYSLSYDEEVGCIGVRGLIRDLEE
                     NNIRPAGCVVGEPTSMQPIIAHKGTHRFRCTVRGREAHSSYVTHGVNAIEYAARLVVF
                     IRQIADRLAQSEIRDYGFTVPYSTLSTGVIHGGIAANVIPKECVFQFDMRTLPQTSPE
                     ALYQEILAQAQRLSGEMHAVDRESGIDLDWVSQTVGLAASESDAIVQWAKQLSRNTAV
                     GKVSYGTEAGLFQKMGVPAVICGPGDIAQAHRPNEFVALEQLAQCEKFMDRILETGYA
                     A"
     gene            1617..3668
                     /locus_tag="VGE92_04750"
     CDS             1617..3668
                     /locus_tag="VGE92_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEY1313160.1"
                     /translation="MAATPLSAVTRVKGACPHDCPDTCGLDVHVKDGVAIKVTGSAQH
                     GPTAGVLCTKVARYTERTYHPDRLLHPMRRIGRKGEGRFEAMSWDAAIAAIVDQLTPL
                     AAANPEQILPYSYAGTMGLVQGESMSMRFFHKLGASLLDRTICASAGTAGHEITLGSR
                     IGVDMELAQDAKLIIFWGSNAITSSVHFWARAQEAKRRGATLVAIDPYRSLTAEKCHT
                     HIALLPGTDSALALGLMHVLIRDDLIDHDYVERHTQGFEALRERVSRYDCARVASICG
                     ITPQEIESLARLYGTARPALIRANYGMQRVRGGGMAMRNVACLPALTGAFRDAAGGIF
                     LSTGGNFNIDRAALERPDLLAGRTPRTINMSTIGHALLDTQPPIKALIVYNSNPVAVA
                     PDSGRVTRGFARDDLFTVVLEHFQTDTADYADILLPATTQLEHLDVIKPYGHYYMMAN
                     NPAIAPLGAAKPNSEIFRLLAKAMGFTEPCFSDSDERIAQAAVAKGWDFDAVRAAGWQ
                     RVGPPAAVARFADGGFDTPSGKVEFFSPRAQALGLDPLPDYIEPQEDTRSAAAIRFPL
                     AMISPPARNFLNSSFVNVQSLRAGEGEPWLDIHPEDAAQRGVSPGQYVRVFNDRGSVE
                     LRARVTDRARRGVVVGLSVWWRKLARDGKNANELTSSDTLTDMGRAPTFYDCLVQVQA
                     L"
     gene            complement(3846..>5117)
                     /locus_tag="VGE92_04755"
     CDS             complement(3846..>5117)
                     /locus_tag="VGE92_04755"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEY1313161.1"
                     /translation="GLFADGSSIKWIAAAPQKWSHLSDAAAQLRSHGVQYAALFVLWA
                     VALGIGAAALGIKLTRFLPAFAVLYVGSAAIYFLGLWDQAAHYNLEPPLVALGLGLLI
                     ANTLGVPKWLAPALRVEFYIKTGIVLLGAGLPLTLIAWGGPVAILQAAIVSLVTFGVI
                     YFSAVRLGLDRRLAATLGTGGAVCGVSGAIAIGGAVGAKREDVSTAISLVVVWAIVMI
                     FALPLAARALHLSTGVAGAWIGTSEFADAAGLAAAQTYGGYAGNIPGIAGTADTAVAA
                     FTLMKVIGRDVWIGVWAFVLSLIATTRWERTGVHSRADAGEIWRRFPKFVLGFLIASA
                     VITLVSKGFDYATYKKEVVPSLVAPLQTFRTWAFTFAFLSIGLTTKLREFAAVGARPF
                     YAFTLGVLVNVVLGYVLSTQVFANFWSHLGQ"
BASE COUNT         1002 a         1596 c         1612 g          907 t
ORIGIN      
        1 cacgaggagc gccaattcgg ccaccccgct tggcaagccg gcaagaagac ctttgcattg
       61 gcgcggcatg acggccggcg cctgacgctg tgtttttggg tgggcgtcga tcagcagggt
      121 ctgctcgttg cggatgctcg atatcacgtc ccgccctaca tgggccacaa cggttggatc
      181 gcgctcgagg tcggcaccga ttgcgactgg tcggagatta aggccttgag cctgcaaagt
      241 taccgtcatt ttgcgctcaa gcggatgctt gacaagctcg ggcctgaatg gagtaaagag
      301 taacaatatt aattgcttat aagacaaatc tcactcttaa tgtaatgtag tgccaggctc
      361 ggcatccggc ccattcgcgg ccaccggcgg ggcgagggct gatgtaaagt aagtgtttga
      421 tcgcgagaga gaacagtgag tacagaacgg cccagtgcgg cgtcgcgcgc catgatcgag
      481 cggctgatcg gtttcaatac cgtgtcgcgc gattcgaacc tcggcttgat cgaatgggtc
      541 cgcgattacc tgcgcggctt cggcgccacg actcgcttga cttacgatgc caccggcaag
      601 aaggcgaatc tcttcgcgac tctgggggat tcgaagaaac ccggtctgat tctctcgggg
      661 cataccgacg ttgtcccggt ggacggtcag gcctgggata cggacccctt tctctgcagc
      721 gaacgcgacg gcaaattatt tgcacgcgga tctgcggaca tgaaaggatt catcggcatt
      781 attttggcgc aagcgcccaa gttcgttgcc gcgttgaatg acaaccggct cgatgcgccg
      841 cttcactatt cactgagcta cgacgaagaa gtcggctgta tcggcgtgcg cggtctgatc
      901 cgcgacttgg aggagaacaa tatcaggccg gcgggatgcg tggtgggcga accgacctcg
      961 atgcaaccca tcatcgcgca caagggaacg catcgcttcc gctgcacggt gcggggccgg
     1021 gaggcgcatt caagttatgt gacccacggc gtcaacgcca tcgaatacgc cgcgcgcctg
     1081 gtggtattca ttcgccagat tgccgaccgc ctggcgcaga gcgagatccg cgactatgga
     1141 tttacggtgc cctattcgac cttgtcgacg ggagtgatcc acggcggaat agccgccaat
     1201 gtgatcccca aagaatgcgt ttttcagttc gacatgcgca ccttgccgca gacttcgccc
     1261 gaggcgctgt accaggagat tctcgcccag gcgcagcgcc tgagcggcga gatgcatgcg
     1321 gtggatcgcg aatcgggcat cgatctggat tgggtgagtc aaaccgtggg gcttgccgcc
     1381 tccgagagcg acgcgatcgt gcagtgggcg aagcaactat cgagaaacac cgcggtagga
     1441 aaggtgtcgt acggaaccga agcgggccta ttccagaaga tgggagtgcc ggcggtcatt
     1501 tgcgggcccg gtgacatcgc gcaagcccat cggcccaacg aattcgtcgc gctcgaacaa
     1561 ttggcacagt gcgaaaaatt catggatcgc attctcgaga caggctacgc agcgtaatgg
     1621 cggccacccc gctatctgcg gtaactcggg tcaagggcgc ttgcccgcac gattgtccgg
     1681 acacctgcgg gctcgacgtg cacgtcaagg acggcgtagc gatcaaagtg acgggttccg
     1741 cgcaacacgg acccacggcg ggtgtgctgt gtaccaaagt ggcgcgctac accgagcgta
     1801 cctaccatcc cgatcggctg ctgcacccca tgcggcgcat tggcaggaag ggcgaggggc
     1861 gcttcgaggc gatgagctgg gacgccgcga tcgctgccat cgtcgaccaa ctcacgccgc
     1921 tcgcggccgc taatcccgaa caaattctac cctacagcta cgcgggcacc atgggcttgg
     1981 tgcaaggcga atcgatgtcc atgcgattct tccacaagct cggtgccagt ctcctcgacc
     2041 gcacgatctg cgcctcggcc gggaccgccg gccacgagat cactctcggc agccgcatcg
     2101 gggtcgacat ggaattggcg caggacgcga aactgatcat tttttggggc tcgaacgcga
     2161 ttacgtcgag cgtgcatttc tgggcgcgcg ctcaggaggc caagcgccgc ggcgctaccc
     2221 tcgtcgcgat cgatccgtac cgctcgctga ccgcggagaa gtgccacacg cacatcgcgt
     2281 tgttgccggg caccgattcg gcgcttgcct tgggcctcat gcacgtcttg attcgcgatg
     2341 atctcatcga tcacgactac gtggaacgtc atacgcaagg gttcgaggcg ctgcgcgaac
     2401 gggtgagtcg gtacgactgc gcccgggtcg cgtccatttg cggcattaca cctcaggaaa
     2461 tcgaatcgct ggcgcgcttg tatggcacgg ctcggccggc gttgatccgc gccaactacg
     2521 gcatgcagcg cgtccgcggc ggcggtatgg cgatgcgcaa cgtcgcttgt ttgccggcgc
     2581 tcacgggtgc gtttcgcgat gccgcgggcg gcatctttct gtcgaccgga ggcaatttca
     2641 acatcgatcg ggccgccctc gagcggcccg atcttctcgc cgggcgcacg ccgcgcacca
     2701 tcaacatgtc gaccatcggt catgcactgc tcgatacgca gccgccgatc aaggcgctga
     2761 tcgtgtacaa ctccaatcca gtggctgtgg cccccgactc cggccgcgtg acgcgaggct
     2821 tcgcgcgcga cgatctgttc accgtggtcc tcgagcattt tcaaaccgac acggccgatt
     2881 acgccgatat cctcctgccc gccaccacgc agttggagca tctggatgtg atcaagccct
     2941 acggccacta ctacatgatg gccaataatc cggcgattgc gccgctcggc gcagcgaagc
     3001 cgaactcgga aatctttcgc ctgctcgcca aggccatggg tttcaccgag ccctgcttca
     3061 gcgattcgga cgagcgcatt gcgcaggcgg cggtcgcgaa gggctgggac ttcgacgcgg
     3121 tgcgcgcggc gggttggcaa cgcgtcgggc cgcctgcggc ggttgcacga tttgccgacg
     3181 gcggcttcga cacgccgtcg ggcaaggttg agttcttttc accgcgcgcg caggcgcttg
     3241 gcctcgatcc gctgcccgac tatatcgagc ctcaggaaga cacgcgcagt gcggccgcga
     3301 tccgctttcc gcttgcgatg atttcgccgc ccgcgcgcaa cttcctcaat agttcgttcg
     3361 tcaacgtgca gagcctgcgt gccggcgagg gtgaaccgtg gctcgacatt caccccgagg
     3421 atgccgcgca acgcggcgtg agccccggtc agtacgtgcg cgtgttcaac gatcgaggca
     3481 gtgtcgagct gcgggcgcgc gtcaccgatc gcgcgcgccg cggggtggtg gtcggattgt
     3541 cggtctggtg gcgtaagctg gcccgcgacg gcaagaatgc caacgaactc acgagcagcg
     3601 atacactcac cgatatggga cgcgcgccga cgttctatga ttgcctggtg caggtccaag
     3661 cgctatgagg gcgcacgcgc tcgtggcggc gacgtagcga tcgtgcatgg cgcaaaggcc
     3721 gtcggcggcg cgcacggccg cgtacgccga actcaagctg gacaatcccg gcagcgctgc
     3781 ttcgagatcg acggggcggc tgttgaaata gcgacactct gcgcactgat tgcggcgcag
     3841 cgaattcatt ggccgagatg gctccagaaa ttggcgaaca cctgggtcga gagaacgtaa
     3901 ccgagcacga cattgaccag cacgcccaaa gtgaaagcgt agaaaggccg cgcgccgact
     3961 gcggcaaatt ctcgcaattt ggtggtgagc ccgatgctta gaaacgcaaa ggtgaacgcc
     4021 caggttcgaa acgtctgcag cggtgcgacc agactcggca ccacctcttt cttataggtg
     4081 gcgtagtcaa agcccttgga caccaaggtg atcaccgccg aggcgatcaa gaatccgagc
     4141 acgaacttcg gaaatcggcg ccagatctcg ccggcgtccg cgcggctgtg cacgccggtt
     4201 cgctcccaac gggtggttgc gatcagcgac agcacgaagg cccacacgcc gatccacaca
     4261 tcgcggccga tgactttcat gagcgtgaat gcggcgaccg ccgtgtccgc agtgccggcg
     4321 atgcctggaa tgttgcccgc gtaaccgccg tatgtctgcg cggccgcaag tcccgccgca
     4381 tccgcaaatt ccgacgtgcc gatccaggcg ccggcgacgc ccgtcgacaa gtgcaaggcg
     4441 cgcgccgcca acggcaatgc gaaaatcatc acgatagccc agacgacgac caaggatatc
     4501 gccgtggaga cgtcctctcg tttggcgccc acggcgccgc cgatggcgat ggcgccggac
     4561 acgccgcaca ccgcgccgcc ggtgcccaag gtcgcggcca gacgccggtc caaccccaag
     4621 cggacggcgc tgaaatagat cacgccgaag gtgacgagcg aaacgattgc cgcttgcagg
     4681 atggccaccg gacctcccca cgcgatcaag gtgagcggca agccggctcc gagcaatacg
     4741 atgcccgtct ttatgtaaaa ctcgacgcgc agcgcgggcg caagccactt cggcacgccc
     4801 agggtattgg caatgagcaa ccccaatccc agtgccacca gtggcggctc cagattgtag
     4861 tgcgccgctt gatcccacag gccgaggaaa taaatggccg cggagcccac atacagaacg
     4921 gcgaacgccg gtaggaagcg ggtcaacttg attccaaggg ccgcggcgcc gatccctaaa
     4981 gccaccgccc atagcacgaa tagcgccgcg tactgcacgc cgtgcgatcg cagttgcgcg
     5041 gccgcgtcgg acagatgact ccatttttgc ggggcggcgg caatccattt gatgctgctg
     5101 ccgtcggcga acagccc
//
LOCUS       DASWZT010000180         4663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_59358, whole genome shotgun sequence.
ACCESSION   DASWZT010000180 DASWZT010000000
VERSION     DASWZT010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59358"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VGE92_04760"
     CDS             complement(<1..465)
                     /locus_tag="VGE92_04760"
                     /inference="COORDINATES: protein motif:HMM:NF017508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HEY1313162.1"
                     /translation="MQVSVESISKLERRMQVQVPAERVTKEIAARLKSLSRTARLNGF
                     RPGKAPLKVIRQQFGSQVHREVIGELLQSSFAEAVTEKQLSPAGNPRIEPQSVGEGQD
                     LTYIATFEVFPEVALQPFDSLQVERISAEVTDGDVDAMIERLRKQQMKYTPVE"
     gene            complement(491..575)
                     /locus_tag="VGE92_04765"
     tRNA            complement(491..575)
                     /locus_tag="VGE92_04765"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(539..541),aa:Leu,seq:tag)
     gene            complement(628..1053)
                     /locus_tag="VGE92_04770"
     CDS             complement(628..1053)
                     /locus_tag="VGE92_04770"
                     /inference="COORDINATES: protein motif:HMM:NF042861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre-like helix-turn-helix
                     domain-containing protein"
                     /protein_id="HEY1313163.1"
                     /translation="MPSKPRTGPRLVLQPASRRAGKHTSSSALKRFFAIADAWELSPL
                     QQCRLLKIPSPKVLERYRLGQAPRLSATQQERAALIANIQFSLTAGAGLRDRAWAWVH
                     APRDAAPFHGDSPLYFMLENGLPALREVARLLVRESEPR"
     gene            1139..1987
                     /gene="pyrF"
                     /locus_tag="VGE92_04775"
     CDS             1139..1987
                     /gene="pyrF"
                     /locus_tag="VGE92_04775"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEY1313164.1"
                     /translation="MPQTFAERLARAHLTSGSLVCVGLDPEIEKLPVDLAADRAPILA
                     FNRRIIDATADVAAAYKPQIAFYGAQAAEDQLLASIRYIRERAPAAIVILDAKRSDIG
                     NTAAAYAREAFDRYGADAVTVNPYMGEDSVRPFLARPERGAVLLCRTSNSGARDFQDL
                     SVDGLPLYRRVAERATAHWNEHRNLMLVVGATYPGEMADLRRAHPNVPFLVPGIGAQG
                     GDLEATLEAGLDARGEGLLISSSRNIIYADGGSAAGIRAAALQLCAAINRRRTLRPER
                     VAAGAA"
     gene            complement(1984..2775)
                     /locus_tag="VGE92_04780"
     CDS             complement(1984..2775)
                     /locus_tag="VGE92_04780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003300391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY1313165.1"
                     /translation="MDLQLAKKTALVSGSTAGIGNAIATSLAREGATVIVNGRTAAAV
                     DAAVAAIKADARGAVVGFAGDLAQASVADALLERHPAVDILVNNLGIYEVKPFEQISD
                     ADWRRLFDVNVLSGARLSRLYLPSMRRRNWGRIIFISSESGYQIPVEMIHYGVTKAAQ
                     IAVARGLAETTSGTGITVNTVLPGPTRSRGVGEFVAEMASQSGKSAASVESEFFKTAR
                     PTSLLQRFATTEEVAAMVTYIASPLSSATNGAALRVDGGTIKTAF"
     gene            complement(2792..>4663)
                     /locus_tag="VGE92_04785"
     CDS             complement(2792..>4663)
                     /locus_tag="VGE92_04785"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEY1313166.1"
                     /translation="APGMDLAAAAARELHYEETNWKARPDISPDGSRLVFSSYLGRSW
                     HNLWVMPAAGGDAFPVGYGDYDQTYPRWSPDGTRIAFISNQNGNTAIGVEQIPGGIVE
                     TLEPRERRYLAPMARLHIDLRDGQGNAAAARVSVTDAGEHVYAPENAWIGADDSFDRS
                     ERHVEAHYFHASGEAYIDVPAGNVNVDILHGFKRRFEQRKLVLQAGQSTDLRVDLDQG
                     KWSVPDAAHWVSADVHVHMNYGGEYRNTPAHLAMQAQAEDLDIVNSLLVNKEQRFPDI
                     AYNGRQLDSASKPGALVVHGQEYHTSFWGHLGLLDISGGVILPGYAGYPNTAASSLYP
                     MNADIADIAHARGALVGYVHPFDEYPEPVAKPGEPLTSELPVDVALGKVDYMEILGFS
                     DHRATAHVWYQLLNLGFRIPAAGGTDAMADFSSLRGPVGMNRVFARVSGAGLDPQQWM
                     AALKAGRSFATNGPLLGFTLGGAEIGDELKFDGAQRGVEFSVRLRSIVPVDHLELVCN
                     GRVIRSFIAAHRTLDHGDFSGRVALEDSGWCVARASTEAGRYPVLDTYAYATTSPIYV
                     SVAGVKPHSAVDARYFAAWIDRTTQTTAAYPDWNNAAEKRAVLERLAQAKAVFIGLE"
BASE COUNT          844 a         1547 c         1413 g          859 t
ORIGIN      
        1 ctccacaggg gtgtatttca tctgttgctt gcgcaaccgc tcgatcatcg cgtccacgtc
       61 gccgtcggtg acttccgccg aaatccgctc gacttgcagc gaatcgaagg gttgcagcgc
      121 cacctcggga aacacctcga acgtcgcgat gtaggtgagg tcctgaccct cgcccacgct
      181 ttgcggctcg atacgtggat taccggccgg cgacagttgc ttctcggtca ccgcttcggc
      241 gaagcttgac tgcagcagct cgccgatcac ctcgcggtgc acctgcgaac cgaactgctg
      301 gcggatcacc ttgagcggcg ctttgccggg acggaagccg ttgagccgtg cggtccggct
      361 caagctcttc aggcgcgcgg cgatttcctt ggtgacccgt tcggccggca cctgcacctg
      421 catgcgccgc tccagcttgc tgatactctc aaccgaaacc tgcatgtcaa tcctcttgaa
      481 tccaatgccg tggtgcgaaa ggcgagactc gaactcgcac gggttacccc accagatcct
      541 aagtctggcg cgtctaccaa ttccgccaca ttcgcatcgg ggttagctct tttgaatagc
      601 ccgctagtat aaccgaggca tcaggcgcta gcgcggctcg ctttcgcgaa ccagcaggcg
      661 agccacttcg cgtaacgccg gcaggccatt ttcaagcata aaatacagcg ggctgtcgcc
      721 gtggaatggc gctgcgtcac gcggtgcgtg gacccacgcc caagcccggt ccctgagccc
      781 ggcgcccgcc gtcaaggaga attggatatt ggcgatcaac gccgctcttt cctgctgcgt
      841 cgccgaaaga cgcggtgctt ggcccaaacg gtaccgctcc agcaccttcg gggaaggaat
      901 ctttagcagc cggcactgct gtaagggcga taattcccac gcgtcggcaa tcgcgaaaaa
      961 tcgcttcagc gcgctgctgc tggtgtgttt gcccgcgcgg cggctcgccg gctgcaatac
     1021 caatcggggt ccagtacggg gcttgctggg cactgcggtt ctcacgctaa cagccgagca
     1081 tactccctaa aggcgtggtc gctcgccatc ctgggcgcgg gcccgctatg atgcgcatat
     1141 gccccagact ttcgccgaaa ggctcgcccg agcgcacctc accagcggca gcctggtgtg
     1201 cgtcggtctc gacccggaga tcgagaaact gccggtcgac ctggcagcgg accgtgctcc
     1261 catactggcg ttcaatcggc ggatcatcga cgcaacggcc gacgtcgcgg ctgcttacaa
     1321 accgcagatc gccttctatg gcgcgcaggc agccgaggac caattgctgg cgagtatccg
     1381 ctacattcgc gagcgcgcgc cggccgccat tgtcattctc gatgcgaagc gcagcgacat
     1441 cggcaacacc gccgcagcct acgcgcgcga ggcgtttgat cgctacggcg cagatgcggt
     1501 caccgtgaat ccctacatgg gtgaggattc ggtacggccg tttctggccc gcccggagcg
     1561 cggcgcggtt ctcctgtgcc gcacgtcgaa ttccggcgcc agggattttc aagacctcag
     1621 cgtcgacgga ctgccgctgt atcgtcgcgt ggcggagcgc gcaacggcgc actggaacga
     1681 acaccgaaat ctcatgttgg tggtgggtgc cacctacccg ggtgaaatgg ccgacctgag
     1741 acgcgcgcat cccaacgtgc cgttcttggt gccggggatc ggagcgcagg gcggcgatct
     1801 cgaggccacc ctcgaggcgg gactggacgc gcgcggcgag ggtctgctga tcagttcctc
     1861 gcgcaacatc atctatgccg acggcggttc cgcggcgggc atccgtgcgg cggcgctgca
     1921 actgtgcgct gccatcaacc ggcggcgcac gctgcgtccc gaacgcgtgg ccgctggcgc
     1981 cgcttagaat gccgtcttga tggtgccgcc gtcgacgcgc agcgccgcgc cgttggtcgc
     2041 ggaagacagg gggctcgcga tataagtgac cattgccgcg acttcctcgg ttgtggcaaa
     2101 gcgttgcagc agcgaggtcg gccgcgcagt tttgaagaat tcgctttcga cacttgcagc
     2161 actcttgccc gattgcgacg ccatctcggc gacgaattct cccacgccgc gcgagcgcgt
     2221 cggccccggc agaacggtgt tcaccgtaat tcccgtgccc gaggtggtct ccgccaagcc
     2281 gcgcgccact gcgatttgcg ccgccttggt tacgccgtaa tggatcattt ccacgggaat
     2341 ctgatagcca ctctcgctcg aaatgaaaat aatgcggccc caatttcggc ggcgcatcga
     2401 cggcaaatac aggcgcgaca gccgcgcgcc gctgagcacg ttgacgtcaa acaaacgccg
     2461 ccaatccgca tctgaaatct gctcgaacgg cttgacttcg tagatgccca gattgttcac
     2521 caatatatcc accgccggat gccgctcaag caacgcgtcg gcgaccgacg cctgcgccag
     2581 gtcgccggcg aagcccacga cagcgccgcg cgcatcggcc ttgatcgccg ccaccgctgc
     2641 gtcgacggcc gccgctgttc ggccgttgac gatgaccgtg gcgccttcgc gcgccaagga
     2701 agtcgcaatg gcgttgccga tgccggcggt cgagccgctg accagggccg tttttttcgc
     2761 taattgcagg tccatgaaat ctcgctcctc gctactccaa gccgatgaac accgccttcg
     2821 cctgcgccag ccgttccaac acggcacgct tctccgccgc attgttccag tccgggtacg
     2881 ccgccgtcgt ctgcgtcgtc cgatcgatcc acgccgcgaa atatcgggca tctacggctg
     2941 aatgcggctt gactcccgcc accgagacat agatggggct ggtggtcgca tacgcatagg
     3001 tatccaacac ggggtagcgt cccgcctcgg tggaggcgcg cgcaacgcac cagccgctgt
     3061 cttcgagcgc aactctgccg ctgaaatcac cgtgatccag cgtgcggtgc gcagcgatga
     3121 aagaacggat cactcggccg ttgcacacga gttcaagatg atcgaccggg acaatggagc
     3181 gcagtcgcac cgagaattca acgccgcgct gcgcgccgtc aaatttcaat tcgtcgccga
     3241 tttccgcacc ccccaacgtg aagccgagca gcggcccgtt ggtcgcaaag ctgcgtcccg
     3301 ccttgagggc agccatccat tgctgcgggt cgagcccggc gccggacact cgcgcaaaga
     3361 cccgattcat gccgaccggt ccgcgcagcg aggaaaaatc cgccatggca tcggtgccgc
     3421 cggccgccgg tatgcgaaat cccaagttga gcagctgata ccacacatgg gcggtggcac
     3481 gatgatcgct gaagccgagt atctccatat agtcgacctt gccgagcgca acatcgaccg
     3541 gtaactcgct ggtcagcggc tcgcccggtt tcgcgaccgg ttccgggtac tcgtcgaagg
     3601 ggtgcacgta accgaccagt gcgccgcgcg cgtgcgcgat atccgcgata tccgcgttca
     3661 tcggatatag gcttgacgcc gcagtattgg ggtagcccgc ataacccggc aggatcaccc
     3721 cgccggaaat atcgagcaac cctaaatggc cccagaagct cgtgtgatac tcctgtccgt
     3781 gcacgaccag ggcgcccggc ttggacgcag aatcgagctg ccttccattg taggcaatgt
     3841 cgggaaagcg ctgctccttg ttgaccagca aggagttcac gatatcaagg tcctccgcct
     3901 gcgcctgcat ggcgaggtgc gcaggggtat tgcggtactc gccgccgtag ttcatgtgca
     3961 catgaacatc cgcgctgacc caatgggcgg catccggaac ggaccacttc ccttggtcga
     4021 gatcgacccg cagatctgtg ctctgcccgg cttgcagcac gagcttgcgc tgttcaaaac
     4081 gacgtttgaa tccgtgcaga atatccacat tcacgttgcc cgcaggcacg tcgatataag
     4141 cctcgccgct cgcgtggaag taatgcgcct cgacgtgccg ctcgctgcga tcgaaggaat
     4201 cgtcggcacc gatccaggcg ttctcgggtg catacacgtg ttccccggca tcggtgacgc
     4261 tgacccgcgc cgctgccgcg ttgccctggc cgtcccgaag atcgatatgc aggcgcgcca
     4321 tcggcgccag gtagcgccgc tcgcggggct cgagcgtctc gacgattcct ccagggatct
     4381 gctcgacgcc gattgccgtg ttgccgtttt ggttcgatat aaacgcgatt cgcgtgccgt
     4441 ccggcgacca gcggggatag gtttgatcgt aatccccgta gcccaccgga aaagcgtcgc
     4501 cgccggccgc gggcatcacc cacaagttgt gccacgagcg gccgagatag gaactgaaca
     4561 ccagacgcga accatctggg gagatgtccg gccgcgcttt ccagttggtc tcctcatagt
     4621 gcaattcgcg tgcggcggcg gccgccaaat ccatgcccgg cgc
//
LOCUS       DASWZT010000181         2377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_35168, whole genome shotgun sequence.
ACCESSION   DASWZT010000181 DASWZT010000000
VERSION     DASWZT010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35168"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VGE92_04790"
     CDS             <1..433
                     /locus_tag="VGE92_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313167.1"
                     /translation="LEQAVYDGVVAGRIRNSADLDALSLSIWRKYEIWPATEPQFGHM
                     WATKSLMFEDPLYLVNYLYAGLLATKMFDLVEREPEAFQKRYADLLREGFYAPPEDLL
                     RSVFGRSLSQHELVDDGLNILRRRMDALAAAYKGLDGSHHQ"
     gene            590..>2377
                     /locus_tag="VGE92_04795"
     CDS             590..>2377
                     /locus_tag="VGE92_04795"
                     /inference="COORDINATES: protein motif:HMM:NF015279.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEY1313168.1"
                     /translation="MSAVSIAAKPIYQPAHKVRFVTATSLFDGHDASVNIMRRVLQAS
                     GAEVIHLGHNRSVQEIVNAAVQEDVQGVAITSYQGGHVEFFKYMMDLLRQNGGDNIKV
                     FGGGGGVIVPAEIRELMAYGVTRLYSPEDGAKLGLQGMIDDVIRSSDYDLGALAPAEG
                     EAMQELRSGSRRQLARVITAVENRLYSMRVRQSLLQAAAAIKTPILGITGTGGAGKSS
                     LTDELILRLRLDQQDRLRVAIISIDPSRKRTGGALLGDRIRMNAIESDKIFMRSLATR
                     DTGTEISAALPEVLACCKISGFDLIIVETSGIGQGESAIVPLVDLSVYVMTPEFGAAS
                     QLEKIDMLDFADFVAINKFDRKGSEDALRDVRKQYQRNHEWFTRDPATMPVYGTMASR
                     FNDDGVTALYQAILPRLQEKGLKLGSGKLPQANVKASSRGRAIVPPERVRYLAEIAEL
                     LREFHRSVDAESRIARERQSLRTAKALFAASGRSLVGFDELIDAKSAELKPACQKLLQ
                     LWPKTQELYAQSEYVVKIRDKEIRTQLITESLSGTKIRKVSLPRFADEGEILRFLLKE
                     NLPGSYPFTAGVFAFKREGEDPTRMFAGEG"
BASE COUNT          459 a          699 c          752 g          467 t
ORIGIN      
        1 cctcgagcaa gccgtgtatg acggcgtggt ggcaggccgc attcgtaaca gcgccgacct
       61 cgatgcgctc agcttgtcga tctggcgcaa gtacgaaatc tggccggcga ccgagccgca
      121 gttcggccac atgtgggcga ccaagagcct gatgttcgag gaccctctgt acctcgtgaa
      181 ctatctgtac gcggggctgt tagcgaccaa aatgttcgac ctggtggagc gcgagccgga
      241 ggcttttcaa aagcgctatg cggatctgct gcgcgagggt ttctacgccc cgcccgagga
      301 cctgctgcgc agtgtgtttg gccgctcttt atcgcagcac gagctggtcg atgacgggct
      361 gaatatcttg agacggcgga tggatgcgct ggcggcggct tacaagggcc tcgacggcag
      421 tcaccatcag tgaaagtgca tataaaatag tgggttatca tatatagttg atgtgatttg
      481 agctagcttc gtgttgcaaa ttcgctcaat ggcggcggcg tattcgggca tttgtttcgc
      541 tgcttgggtt ctctaatata aatcgctgaa ttcacagagg cagatcctta tgagcgccgt
      601 atccatcgct gccaagccga tctatcagcc cgcgcacaag gttcgattcg tcacggccac
      661 ctcgctgttc gacggacatg atgcctccgt caacatcatg cggcgggtgc tgcaagcctc
      721 gggggccgag gtcatccatc tggggcacaa ccgctcggtg caggagatcg tcaacgccgc
      781 cgtgcaggaa gacgtgcaag gtgtcgccat cacctcgtat caaggcggtc acgtcgagtt
      841 cttcaagtac atgatggatc tgctacggca gaacggcggc gataacatca aggtgttcgg
      901 cggcggcggc ggcgtcattg tgccggcgga gattcgcgaa ctgatggcct atggggtgac
      961 acgcctgtat tcgcccgaag atggcgccaa gttgggattg caggggatga tcgacgatgt
     1021 cattcgcagc agcgactacg atctcggggc gctggcgccg gccgaaggcg aggccatgca
     1081 ggaattgcgc tcggggagtc ggcggcagct ggcgcgggtc atcaccgccg tggaaaatcg
     1141 cctatattcc atgcgcgtgc gccagtcgct gctgcaggcc gcggccgcca tcaaaacgcc
     1201 gattttgggg atcaccggca ccggcggtgc cggcaagtcg tcgctgacgg atgagttgat
     1261 tctgcgcctg cgcctcgacc agcaagaccg gctgcgcgtc gccatcatct ccatcgatcc
     1321 gtcgcgcaag cgcaccggcg gcgcgctgct cggcgatcgt attcgcatga atgcgatcga
     1381 gtcggacaag atattcatgc gctcgctggc cacgcgcgac accggcaccg agatatcggc
     1441 cgcgctgccc gaagtgctgg cgtgctgcaa gatctcaggc ttcgacctca tcatcgtgga
     1501 gacttccggt atcgggcagg gcgagtccgc catcgttccg ctggtggatc tgtcggtgta
     1561 tgtgatgacg ccggaattcg gcgcggcgtc gcaattggaa aagatcgaca tgctcgattt
     1621 cgcggatttc gtcgccatca acaaattcga ccgcaagggc tccgaagatg cgctgcgcga
     1681 cgtgcgcaaa caatatcaac gcaatcacga atggtttacg cgcgacccgg cgaccatgcc
     1741 tgtatatggc accatggcct cgcgcttcaa cgacgacggc gtcaccgcgc tctatcaagc
     1801 catcctgccg cgcctgcagg agaaggggct caagctcggc agcggtaagt tgccgcaggc
     1861 aaacgtcaaa gcctcctcgc gcggccgcgc catcgtaccg ccggagcgcg tgcgctatct
     1921 ggcggaaatt gccgagctgc tgcgcgaatt tcatcgctcg gtggacgctg agtcgcgcat
     1981 tgcgcgcgag cggcagtcgt tgcgcacggc caaggccttg ttcgccgcat ccggccgcag
     2041 cctggtcggc ttcgatgaat tgatcgatgc caaaagcgct gaacttaagc ccgcttgcca
     2101 gaaactgctg cagctgtggc ctaagacgca ggagctgtac gcacagagcg agtatgtcgt
     2161 gaagattcgc gacaaggaga tccgcacgca actcatcacc gagtcgctct cgggcaccaa
     2221 gatccgcaag gtgtcgctgc cgcgcttcgc ggacgagggt gaaatcctgc gcttcctgtt
     2281 gaaggagaat ctgcccggct cctacccctt cacggccggc gtatttgcct tcaagcgcga
     2341 gggcgaggat ccgacgcgca tgttcgcggg cgagggc
//
LOCUS       DASWZT010000182         5459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_88323, whole genome shotgun sequence.
ACCESSION   DASWZT010000182 DASWZT010000000
VERSION     DASWZT010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5459
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88323"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="VGE92_04800"
     CDS             complement(<1..1083)
                     /locus_tag="VGE92_04800"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY1313169.1"
                     /translation="MASGRQARIKNFFAENRLFAVRSVVAGIIAATLLLTVAGRLFYL
                     QVLMHDYYASQSLGNSIRTEPIPPSRGLILDRNGVVLADNLPAYQIELVREQLGDRKA
                     LDVTLDQLVDIGLLRREDINNLRRTILLHKVYESVPIKLQLNDAEMALFAVHRYQFSG
                     VDIRTRLARHYPLGQTAVHAIGYVSAINEADMKQIDSDEYAGTTLIGKLGVEAAYEQQ
                     LHGKRGSREILVNAAGRPVERQGEYTPQLTTHPPVAGEDLILGLDVRVQKVAEDALAG
                     KRGSVVALDPRTGDIIALASTPGFDPNAFVRGLTVPEYSALQNDIDIPLLNRALRGAY
                     PPGSTVKPLYALAAQKYNIITPLQTQY"
     gene            complement(1142..2014)
                     /gene="pbpG"
                     /locus_tag="VGE92_04805"
     CDS             complement(1142..2014)
                     /gene="pbpG"
                     /locus_tag="VGE92_04805"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF008668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HEY1313170.1"
                     /translation="MARTPPKPSLPLRALAGICLTLLALQCVAKSNRDPRDPKLKSSS
                     VLVIDQSDSSVLYSRNSDVAMPIASITKLMTALVVLDAKQPLDEPIQLTNADRELSKG
                     GFSRLAVGATLTRGDLMHLALMSSENRAAHALGNHYPGGMAAMVAAMNAKAASLGMSS
                     SHFADPTGLSSDNVASPADLAKLVIAASHIPSIREYSTDSRYSVKIRKRLVEFRNTDN
                     LVANPDWDITVQKTGYTSEAGKCLVMAAVIEGRPVVLVLLDSYGKYTRVADAKRIKTW
                     IQANANQRVAVSMR"
     gene            complement(2145..3143)
                     /locus_tag="VGE92_04810"
     CDS             complement(2145..3143)
                     /locus_tag="VGE92_04810"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY1313171.1"
                     /translation="MGSTRSIEVLCLALAALVLVWSRALATQLWPVLGLLVAFVLAIL
                     AFRSRRFFPGGTRTRLLIESWSMVMFITGALWFSGKSASPLLNLYLLPIILSALTLGR
                     LVTLSQVAVIFVCHVLLAAATPGPEVLSLSYASQAVGQLAPFLLVAFLTTTLSADITE
                     ARERIEDLAQNDALTGLYNVRMFNEVWKREHAASERDRGVYALLMVDIDKLKDINDSF
                     GHEAGNSAITLVARCLQRSIRNTDHAARLAGDEFAVLLPGASPEIAEAVVKRVRHNVY
                     KTTLDLRSRMIRCSVSIGVVNYPKDARDMRELLSIADRKMYRDKELRRAPGSEVSA"
     gene            complement(3145..4395)
                     /locus_tag="VGE92_04815"
     CDS             complement(3145..4395)
                     /locus_tag="VGE92_04815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY1313172.1"
                     /translation="MITKPRHLMFVAAAIAIGASLGVGGAVLADKPAAPGTDLPWQDA
                     RMLADVLERVKHDYVNPVDDHQLLQAAIRGMVSSLDPYSAYLDGDEYDEVKISSSGQY
                     SGVGIEVSMEDDQVVVVSPFEGSPAAEAGIHPGDVIASIDGVPVNTTTLADTIGRMRG
                     KEGTSVKIGILREGASEPLQFTLKRSRVELHSVRSEMLEPGMGYVRISQFSETTGDDL
                     EAALKDLRKRNAGPLKGLVLDLRNNPGGVLEAAVSVSDTFLDSGVIVTAKGRTPESKF
                     EMDATPGDALNGAPIVVLINGGSASAAEIVAGALKDNHRAKLMGRTSFGKGSVQTVIP
                     LSGDRAIKLTTSLYYTPSGISINHRGIAPDIELERDPKPPSVAPPADAPLLQRDPEVR
                     RAVQELKVPTVGGSGSVTAAARTN"
     gene            complement(4430..>5459)
                     /locus_tag="VGE92_04820"
     CDS             complement(4430..>5459)
                     /locus_tag="VGE92_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEY1313173.1"
                     /translation="LSSARGDKPLLVSLSIGVSRLGDRDRPLDHALAAAELACRTAKE
                     RGRNRVEVFYGNAKISEGPAAPGFIAQLNAALRSDSFELLAQPILPLNSSPADPRFEI
                     LLRMRAADGSRLGLEKLIADSGSAELARQVDRWAIEQVVERLAACRPLLQQYPAKFSV
                     NLSAASLADSEFWRMLEDLVRNGRIEPGTLGFEFPEEAASAHLDSIAPCMGKLRDLGI
                     FFALDNFGRAIGSLSHLNSLPVSCIKIDGSFSRNLIDNPKSQSMVLAIAKLAGTFGLE
                     TVAGCIETDAIRARAAQLGVDYGQGFLIGKPLPLDDAVHDLPLYACFETSTGLFDPSL
                     GKTAAPGN"
BASE COUNT         1031 a         1685 c         1739 g         1004 t
ORIGIN      
        1 gtactgggtc tgcagcgggg taatgatgtt gtacttctgg gcggccagcg cgtacagggg
       61 tttgaccgtc gagccgggag gataggcgcc gcgcaaggcg cgattcagca gcggtatgtc
      121 gatatcgttt tgcagtgccg aatattccgg aaccgtcaga ccgcgaacga aggcattggg
      181 atcgaatccc ggcgtgctgg cgagcgcgat gatatcgccc gtgcgcgggt cgagggcaac
      241 gaccgagcca cgcttaccgg cgagtgcatc ttccgcaact ttctgcacgc gcacatcgag
      301 acccaggatc aaatcctcgc ccgcaacggg ggggtgcgtg gtgagttgcg gggtgtattc
      361 gccctgccgt tcgaccgggc ggccggccgc attcactaaa atctcgcgcg atccgcgctt
      421 gccgtgcagc tgctgctcat aggcggcttc cacgcccagc ttgccgatga gcgtggtacc
      481 cgcgtattcg tcgctgtcga tttgcttcat gtccgcttcg ttgatggcgc tgacgtagcc
      541 gatggcatgc acggcggtct ggccgagcgg gtagtgcctg gcaaggcggg tgcggatatc
      601 gacgcccgag aactgatagc gatggacggc gaacaacgcc atctctgcgt cgttcagctg
      661 caatttgatg ggcacgctct catatacttt gtgcagcagg atggtgcgcc tgaggttgtt
      721 gatatcttcg cggcgcagca aaccgatatc gaccagctga tcgagcgtca catcgagcgc
      781 cttgcggtcg cccagctgtt cgcgcaccaa ttcgatctgg taggccggca gattgtcggc
      841 gagcaccacc ccgtttcgat ccagaatcaa accgcggctc ggcggtatcg gctcggtgcg
      901 aatgctgtta cccagcgatt ggctggcgta ataatcgtgc atcaatactt gcagatagaa
      961 gagacgcccc gcgaccgtca gcagcagagt ggccgcgatg atgccggcga cgacactgcg
     1021 aaccgcgaac agacggttct cggcgaagaa attcttgatc cgtgcttgac gaccgctggc
     1081 catgaatcaa tccgtgtgcg gcgcgcgcag ccaccgccgg caagagttgc cgcgcaacgg
     1141 ttcagcgcat cgacactgcg actcgctggt tggcattcgc ctgaatccag gtcttgatgc
     1201 gcttggcgtc ggcaacgcga gtgtatttgc cgtacgaatc caacaacacc aacacgaccg
     1261 ggcgcccctc gatgacggcc gccatcacca ggcactttcc cgcctccgag gtgtaaccgg
     1321 tcttctgcac ggtgatgtcc caatccggat tggcgaccaa attatcggtg ttgcggaact
     1381 cgaccaggcg cttgcgaatt ttcacgctgt agcggctatc cgtggaatat tcgcgaatgc
     1441 tcggaatgtg cgacgcggcg atcaccaatt tggcaagatc ggccgggctc gcgacgttat
     1501 cgctcgacaa accggtggga tcggcgaaat gtgaactcga catgccgagc gaggccgcct
     1561 tggcgttcat ggcggcaacc atcgcggcca ttccgccggg atagtggttg ccgagcgcat
     1621 gcgctgcgcg gttttccgag gacatcaggg ccaaatgcat cagatcgccg cgggtcaggg
     1681 tggcgcccac ggcgaggcgc gaaaaaccac ccttcgacaa ttcgcggtcg gcgttggtga
     1741 gttggatcgg ttcatcgagg ggctgcttgg catcgagcac caccagagcc gtcatcagct
     1801 tggtgatgga cgcgatcggc atcgccacat cggaattgcg cgaatacagc accgacgaat
     1861 cgctttgatc gatgacgagc accgagctgg atttgagttt gggatcccgc ggatcgcgat
     1921 tggacttcgc gacgcactgc agcgcaagca aagtcaggca gatgccggcc agcgctcgta
     1981 acggcaggga cggcttcgga ggggtgcggg ccatgggttt cccaagtgtt caggtgtaat
     2041 acggcaagct gacacaggtc atcccggccg cgacgtgatg caaacctaac tttgccatca
     2101 ttttggcgaa tgtccagcac attcagagca ttggcccgcc aatctcaagc actcacctca
     2161 ctgccgggcg cccgacgcag ctccttgtcg cgatacattt tgcgatccgc gatgctgagc
     2221 agctcgcgca tgtcgcgcgc gtccttcggg taattgacga ccccgatgct cacgctgcag
     2281 cggatcatgc gcgagcgcaa atccagcgtc gtcttgtaga cattgtgccg tacccgcttg
     2341 accacggcct cggcgatctc cggcgaggcg ccgggcagta acaccgcgaa ttcatcgccg
     2401 gccaggcgcg ccgcatgatc cgtattgcgg atgctccgct gcaagcatct tgccaccagc
     2461 gtgatggcgc tgttgcccgc ctcgtgaccg aagctgtcat tgatgtcctt gagcttgtcg
     2521 atgtccacca tgagcagcgc atacacgccg cgatcgcgct cgctggccgc gtgctcgcgc
     2581 ttccacactt cattgaacat ccgcacgttg tagagtcccg tcaaggcgtc gttttgcgcc
     2641 aggtcttcga tacgttcgcg tgcctcggtg atatcggcgg acagggtcgt ggtgaggaat
     2701 gccaccaaca ggaacggcgc cagctgtcct acggcctggc tcgcgtagga cagcgacaaa
     2761 acctcagggc cgggcgtcgc ggcggcgagc agcacgtggc agacaaatat caccgccact
     2821 tgcgataggg tcacgaggcg tccgagggtg agcgcactca gaatgatcgg cagcaaatac
     2881 aggttcagga gcggactcgc gctcttgccg ctgaaccaca aggctccggt gatgaacatc
     2941 accatggacc aggactcgat cagcaaacgg gtgcgggtgc ccccggggaa gaaccggcgc
     3001 gagcgaaacg cgaggatggc gagcacgaac gctaccagca gtcccagcac cggccagagt
     3061 tgcgtcgcga gcgcgcgcga ccacacgagc accaaggcgg ccagcgccaa acacagtacc
     3121 tcgatggagc gggtactacc catgctaatt cgtgcgcgcc gccgcggtta ccgagcctga
     3181 gccgccgacg gtgggtacct tcagttcctg cacggcgcgc cgcacttccg gatcacgctg
     3241 cagcagcggc gcatccgcgg gcggcgccac gctcggcggc ttcggatcgc gctccagctc
     3301 gatatcgggg gcgatacccc gatgattgat ggaaatcccg gagggcgtgt agtacagaga
     3361 cgtcgtgagt ttgatggcgc ggtcgccgct cagaggaatc acggtttgca ccgaaccctt
     3421 gccgaagctg gtgcggccca tgagtttggc tcgatgattg tccttgagcg cgccggcgac
     3481 gatttccgcc gctgaggccg atccgccgtt gatcagcacc acgatcggcg cgccgttgag
     3541 cgcgtcgccg ggcgttgcat ccatttcgaa tttggactcg ggtgtccgcc ccttggcagt
     3601 cacgatgacg ccggagtcga gaaaggtatc ggagaccgag acggccgcct cgagaacacc
     3661 gcccggattg ttgcgcaagt cgagcaccag gcccttcagc ggtccggcat tgcgcttgcg
     3721 cagatccttg agcgcggcct ccagatcgtc gccggtggtc tcgctgaatt gtgaaattcg
     3781 cacatacccc atgccgggtt cgagcatttc cgatctcacg ctgtgcagtt cgacgcgcga
     3841 acgcttcagc gtgaactgca gcggctcgct ggcgccttcg cgcaaaatgc cgattttgac
     3901 cgaggtgcct tctttgccgc gcatgcgccc gatcgtgtcg gccagcgtgg tcgtgttcac
     3961 cgggacgccg tcgatcgagg cgatgacatc gcccgggtga atgccggctt cggccgcggg
     4021 cgaaccttcg aagggcgaca ccaccacgac ctggtcgtcc tccatcgaaa cctcgatgcc
     4081 gacgcccgag tattgtccgg agctcgagat cttgacttcg tcgtactcgt cgccgtcgag
     4141 gtaagccgaa taaggatcca acgatgacac catgccgcga atcgcagcct gcagcagctg
     4201 gtgatcatcc acgggattga cgtagtcgtg cttcacccgc tccagcacgt cggccagcat
     4261 gcgcgcgtct tgccacggca gatcggtccc cggtgccgcg ggtttgtcgg cgagcaccgc
     4321 gccgccgacg ccaagcgaag cgccgatcgc aatggcggcg gctacgaaca tcagatggcg
     4381 cggtttcgtg atcatcgggc atcagcatac gtgcggccgc gacccgccgc tagttgcccg
     4441 gcgccgcggt cttgcccagc gacggatcga atagcccggt tgaagtttcg aagcaggcgt
     4501 acaacggcag gtcgtgaacc gcgtcatcca acggcagcgg tttgccgatc agaaatccct
     4561 gcccgtagtc cactcccaat tgtgcggcgc gcgcgcgaat ggcatcggtc tcgatgcatc
     4621 cggcgacggt ctcgagaccg aacgtgcccg cgagcttggc gatggcgagg accatggatt
     4681 gcgatttcgg gttgtcgatg agattgcggc tgaaactgcc gtcgatcttg atgcacgaca
     4741 cgggcagcga attcaaatgc gacagcgagc cgatggcgcg cccgaaattg tcgagggcaa
     4801 agaagatgcc gaggtcgcgc agcttgccca tgcacggcgc gatgctgtcg agatgggcgc
     4861 tggcggcctc ttccgggaat tcgaagccga gggtaccggg ctcgatgcgg ccgttgcgca
     4921 ccaaatcctc caacatacgc cagaattcgc tgtcggcgag cgaggccgcg gacaggttta
     4981 ccgagaactt cgccggatac tgctgcagca gcggccggca ggcggccaga cgctcgacga
     5041 cctgttcaat ggcccatcga tcgacctgcc gggccaattc ggcagagccg gagtcggcga
     5101 tcagtttctc gaggcccagg cggctgccat ccgcggcgcg catgcgcaat aaaatctcga
     5161 accgcggatc cgcaggcgac gaattcaacg gcaggatcgg ttgtgccaga agttcgaacg
     5221 agtcggatct gagcgccgcg ttcagttgcg cgatgaaacc cggggcagcg ggaccctccg
     5281 agatcttcgc gttaccgtaa aacacttcga cccggttgcg gccgcgctcc ttggcggtgc
     5341 gacaggcgag ttcggctgca gccagcgcat ggtccaaggg ccggtctcgg tcgccgagtc
     5401 gcgaaacccc gatgctcaag gacacgagca gcggcttgtc gccgcgcgcg ctcgagagt
//
LOCUS       DASWZT010000183        12554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_30449, whole genome shotgun sequence.
ACCESSION   DASWZT010000183 DASWZT010000000
VERSION     DASWZT010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12554
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30449"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VGE92_04825"
     CDS             <1..587
                     /locus_tag="VGE92_04825"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY1313174.1"
                     /translation="PALLDSAFTLMCHQLLGFQAIGDLPQRLGSGAPSAAPYRVFAAA
                     DGDFMLATASDAQFTRLCILLDLPAMAHDPRFATMTARLAHREELDSWLADKFVQRPV
                     AAWLQILGQAGLSAGKVNDIREALALPVVAERSFFVAPDALPWAGGLPLLRLPIDPNG
                     AGIRRPPPKLGEHTVEVLREAGMDEAAIARICGD"
     gene            complement(604..1350)
                     /locus_tag="VGE92_04830"
     CDS             complement(604..1350)
                     /locus_tag="VGE92_04830"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY1313175.1"
                     /translation="MSRLTGKIAIITGASQGMGMAHARAFVEHGAKVILGDLNEKSGK
                     ALAAELGANAIFVKQDVSRAEDWRAVLQKGEERFGTVNVLVNNAGILGTLAATIDMPE
                     AEFLRVCAVNQTGVFLGMQTVLPGMIKAGGGSIVNISSIAGLVAIYGAPNLAYVASKF
                     AVRGMTKQVAVEYGAKNIRVNSVHPGYIKTPMMVAATDEGGGEALKQIPLRRMADASE
                     VSNLVIFLASDESAFITGTEHVIDGGMTAL"
     gene            complement(1409..2548)
                     /locus_tag="VGE92_04835"
     CDS             complement(1409..2548)
                     /locus_tag="VGE92_04835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY1313176.1"
                     /translation="MDFQFTHEQRLWGDTVHGFMDKEVGREYTREHDASREFPEEVFK
                     KMAQQGWLGLLVDEQDGGSAADPIMFALFCEAIGKFSLDTAACIMTSMFTATNISRHG
                     TAEQKRRYLEPFLAGNAKFSISISEPQAGSDAAAARSTAVLDGDQWVLNGNKVWCSGA
                     HLPNATIAILARTGPGRHDGFSVFLVPNTTPGLKIKKMNTIVRKSLGTTEFFMDNLRI
                     PKDALLGEAGKGWQYIGEHLEYERLSIAASQVGNARTALDDTIRYTKDRKQFGVPLAS
                     FQVLKHRMAENETELEAARLLVYSAACKMARGERAVKEVSMAKLFCSQVLFRIAFNGM
                     QALGGYAQLPEYDMERYFREAKHGMVGGGTNEIQRSIIAKEMGIR"
     gene            2725..4437
                     /locus_tag="VGE92_04840"
     CDS             2725..4437
                     /locus_tag="VGE92_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY1313177.1"
                     /translation="MPTPLQRRAATEARHPVWRAKTLAQALDSAAAEFAGRPLVITDS
                     RIYTYRDMQNWSRTLAAGLIECGVRAGDHVALLLANFPEFVAIKYAIARAGAVAVPVN
                     FALRRQELSYILDQSDSKLLITMDRLRDRDYLEDLDALVAGWETSGGGARWPNLRRVV
                     VHATEGAQRSRATSLDELNAMATPSSHAELARREGAADPHFRSDVIYTSGTTGLPKGV
                     MLTHDMILRAAYASAYTRAFEDGRRILFALPMYHVFGFVECLIACTFVGGAIVPQLSF
                     DARRMLDAARSHRATEIVCVPMMTLKLLDEARQNGFDGADLLAMFNSGGASPASIWQD
                     IRELLGAREIVTAYGMTETTASTTCTLPEGPDEFLLTTNGRLKFAGVAGDADLGGVLA
                     QYKTVDPATGVDLPFGLPGELVARGPIVTRGYYKKEAETRAAFNADGWLHTGDVGVIT
                     ADGYLTLTGRLKETYRCGGEMVMPREIEDLLNEHPLVAQALVVGLPDVKMGEVGCACI
                     VPAGDVFPEPQELIELCASRLARFKVPRHVIFVTAAELPLTATGRAQKFKLADLAKQR
                     LSIV"
     gene            4448..4744
                     /locus_tag="VGE92_04845"
     CDS             4448..4744
                     /locus_tag="VGE92_04845"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY1313178.1"
                     /translation="MVIERADFAVIAARETEFESVMERGCALLAGASGCTSVALLRCV
                     ERPSRYCLQLGWNSIADHHAFTKTQDFQTFREIAGPFFAERPAMEHFQPIHQRG"
     gene            4741..5061
                     /locus_tag="VGE92_04850"
     CDS             4741..5061
                     /locus_tag="VGE92_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEY1313179.1"
                     /translation="MSEPSMPHIVTDKCQQCRFTDCVTTCPVACFHGDSQMLYIDNVQ
                     CVDCGACVPACPVHAIYEQSELPADKLEWLAINAQRAPGLPVIEDKQVPLPTAEARKR
                     ELGY"
     gene            5045..6466
                     /locus_tag="VGE92_04855"
     CDS             5045..6466
                     /locus_tag="VGE92_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEY1313180.1"
                     /translation="MNSDTDAGALGSAENPLHVAIVGSGPSGFYAAEALLKSSLTVRV
                     DMIERLPVPFGLVRYGVAPDHPRLKQPTLVFDKIAQSPGFTFLGNVTVGRDISLEQLQ
                     STHHAVLFACGAESDRRLGLANEDLPGSHTATEFVGWYNGHPDYCERTFDLSSETAVI
                     IGQGNVAIDVARILGKSVDELRSTDMAEHALDALAHSRVRDIHIIGRRSPAQASFTFK
                     ELRELGELRGCVTRVAAEDLQLNPASLAELEDKTNFNAAKNVEILQSWTLHGAATADK
                     RIWFHFLQSPVALSGKQRLERVTLEKNSLAGPPFGQTAQGTGVSLDLSCGLLFRSIGY
                     RGLPLPGVPFDATKGVFPNTAGRISDTLGLYAAGWIKRGPSGIIGTNRADAVASVQSL
                     LADVPRLDSRPKSGSEGVYAALARIESPVLSYGDWRRIDAEEQRRGEAIGKPREKITR
                     VSDMLALAASPLAETALEHAHHS"
     gene            6483..7223
                     /locus_tag="VGE92_04860"
     CDS             6483..7223
                     /locus_tag="VGE92_04860"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY1313181.1"
                     /translation="MAGRLQGKVALVTGTGSGIGRAIAERFIAEGAQVVAVDISGNQN
                     AVAEKLGPNCVPFQADVSKSADVQAMLALAASKFGALHVLCNNAAIEGHMAPVGEYPE
                     EEWEKVMAINLRSVFLGMRYGIPLMLKSGGGSIVNTASMAAMVAFPNMAAYCAAKGGV
                     KQLTKTAAVEYAAQGIRANAICPGTIKTAITAALPAEYIKAIINANPVKRIAEPVEVA
                     HLAVFLASDESSFITGTEITIDGGYTAL"
     gene            7479..8090
                     /locus_tag="VGE92_04865"
     CDS             7479..8090
                     /locus_tag="VGE92_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313182.1"
                     /translation="MQTPDKPFKTYPLTPQYAAILKQRRDDEAAGKPFQVAQNRCLPA
                     GLAASMTTGAYPLEIFYQKGGHEILFQKENVGALYRVYLNRPHKSADQLYPQFYGDSI
                     GHWEGNTLVIDSVSLGATSAFDLITPHSDALHVIQRLQRPTYDTLQDEITFDDPKALT
                     TPITATAVFKLHADWEIQEYECTNERLIFDEHGNQTISKSTPR"
     gene            8087..8494
                     /locus_tag="VGE92_04870"
     CDS             8087..8494
                     /locus_tag="VGE92_04870"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HEY1313183.1"
                     /translation="MIRPSICLLLLLAVVSPAFAHHSYAMFDANSLITRDATVKEFQW
                     TNPHSWLQVMMLDDKGVVEEWSLELGPLVVLSRWGWKPHSLNPGDKVKVSLNPLRDGT
                     HGGRLMSIVLPDGATLSGQGGAQASASSVPASH"
     gene            complement(8516..9046)
                     /locus_tag="VGE92_04875"
     CDS             complement(8516..9046)
                     /locus_tag="VGE92_04875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEY1313184.1"
                     /translation="MTTAMHKLSTVLAAVTVLISPFANRVVAAHNDTEEVRTVVTGFA
                     TTWNRHDLNAFGKLFATDADFVNVAGIFWKGGKLIQTQHAYIHGVIPADSPGFTKQDR
                     PYWAIFKDSTLKFEPITVRFLCKEVAVAHVNWEMLGDSRTRDPRYGVLLFVLTREDAG
                     WLIAAAQNTEIHRTVN"
     gene            9201..10184
                     /locus_tag="VGE92_04880"
     CDS             9201..10184
                     /locus_tag="VGE92_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012313669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY1313185.1"
                     /translation="MTFQQLRYLVAVHENGLNITTAARELRISQPGVSRQLKLMETEL
                     GFDLFEREGRALVRTTSAGEDIVARATTILRELQNVQRTSAELSRVDGGSLSIATTHT
                     QARYVLPHVVRAFRAKYPKVRLHLHQGTSEQIAEMVARDRVDFAIATGSEELFPHLIR
                     LPVYRWHRTVVVPHGHPLASKGKLTLKKLADYPIVTYVFSFSGRSSLPALFETAGLSL
                     DVALTARDSDIIKTYVRIGLGVGILAKLAVDPVIDSDLVTLDAAHLFEGHTTWIGFRR
                     GALLRAYMYEFIELLASHLPRKLVRAAEKMDTQEDVDRILTGFKVPLRDNT"
     gene            complement(10230..11822)
                     /locus_tag="VGE92_04885"
     CDS             complement(10230..11822)
                     /locus_tag="VGE92_04885"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEY1313186.1"
                     /translation="MFRNYALSVFLVLASAARAADHFDGESWWSTVKILADDKFEGRD
                     TGSTGERQAQAYVVQQLKTLGLEPAGSNGYYQSVKLQTLELDEPSSSLALVRGGRAQP
                     LTLGEQAYFSTRYAQAPKVDAPLVFVGYGLNIPEQAYNDFLGLDLKGKVAVVVTGSPA
                     EVPSALSAHYQSRAERWKALKAAGAIGVISLPNPASMEMSWARMALNRNLPSMNLVGT
                     EFDETSGCQLGVIFNPAHADMLLEGTGHTFAEIAALAKDRKVLPRFPLTASVSAVTHT
                     STRDLQSTNIVARMPGKDPKLKAEYVVLSAHIDHVGVGAPINGDRIYNGAMDNGSGSA
                     LLLDVARSLKRAHVPLKRSVLFVWVTGEEKGLLGSKFFAAHPTVPATSMIADINTDMF
                     LPIIPLKVLTVYGLAESDLGDRVAKIAADQGLRVQPDPAPLHNIFIRSDQYNFIRHGI
                     PSVMIDVGASDPGEEKILKDWHNDRYHAPSDDGNQPHNLATAAGYEEVIRALTIAVAD
                     DPVRPAWKQDSFFRRYAAAGAN"
     gene            complement(11839..12036)
                     /locus_tag="VGE92_04890"
     CDS             complement(11839..12036)
                     /locus_tag="VGE92_04890"
                     /inference="COORDINATES: protein motif:HMM:NF023204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HEY1313187.1"
                     /translation="MAHRWAYGQILPRAYWGASFLLADYWLFALEVPPAGYEWVRDDT
                     DAILVNTSTGEILQVEYGVFG"
BASE COUNT         2497 a         3852 c         3709 g         2496 t
ORIGIN      
        1 gccctgcact gctcgactcg gcgtttactc tgatgtgtca tcagttgctc ggattccaag
       61 cgatcggcga tttaccgcaa agactgggat ccggtgcgcc gagcgccgct ccctatcgcg
      121 tgttcgcggc cgccgacggc gacttcatgc tggccaccgc cagcgacgcg caattcacgc
      181 gcctgtgcat cttgctcgac ctgccggcca tggcgcacga tccgcgcttc gcaaccatga
      241 cggcacgtct cgcgcatcgc gaggaactcg actcatggct ggccgacaag tttgttcagc
      301 gacccgtggc cgcgtggctg caaatccttg ggcaagcggg cctctcggcc ggcaaagtga
      361 atgacatccg ggaagcgttg gccctaccgg tggtggccga acgcagtttt tttgtcgcgc
      421 ccgacgccct gccatgggcg ggcggcttgc cgctgttgcg attgcccatc gatcccaatg
      481 gcgccggcat ccgtcgccca ccgccgaaac tgggcgaaca cactgtcgag gtgttgcgcg
      541 aagcgggcat ggacgaggcg gcgatcgcac gcatctgcgg ggactaacgc cccgaattgc
      601 gcgctacaac gccgtcattc caccatcgat gacatgttcc gtgccggtga tgaatgcgga
      661 ctcgtcagaa gccaagaaaa tgaccaaatt ggacacttcg gacgcatccg ccatgcggcg
      721 caacggaatc tgtttcagcg cttcgccgcc gccctcgtcg gtggcggcaa ccatcatggg
      781 cgtcttgatg tagcctgggt gcacggagtt gacacgaatg ttcttggcac catattccac
      841 cgcaacctgc ttcgtcatgc cgcgcaccgc gaacttgctg gccacatagg cgaggttcgg
      901 cgcgccgtag attgccacga ggccggcgat cgacgagatg ttcacgatcg aaccgccgcc
      961 ggccttgatc atgccgggga gcacggtctg cattccgaga aacacccccg tttgattgac
     1021 cgcgcagaca cgcaagaatt cggcttccgg catatcgatc gtggccgcga gcgtacccag
     1081 aatgccggcg ttgttgacca gcacgttgac ggtgccgaag cgctcttcac ctttctgcag
     1141 caccgcccgc caatcctcgg ctcttgagac atcctgcttg acgaaaatgg cgttggcgcc
     1201 gagttcggct gccagcgcct tgccggattt ctcattgagg tcacccaaga taaccttggc
     1261 gccatgctcg acgaaggcgc gcgcatgcgc cattcccatg ccctgggacg ccccggtgat
     1321 gatcgctatc tttccagtca gtctgctcat ttgtgtaccc tttgcgcagt cgcgctttaa
     1381 aattttatcg ccaactcaag accgaacatc accgaattcc catctccttg gcgatgatgc
     1441 tgcgctgaat ttcgttggtg ccgccgccaa ccatgccgtg cttcgcttcg cggaagtaac
     1501 gctccatgtc atattccggc agctgtgcat agccccccaa tgcctgcatt ccattgaagg
     1561 cgattctgaa aagtacctgc gagcaaaaca gcttcgccat tgaaacctcc ttgaccgccc
     1621 gctctccgcg cgccattttg caggcggcgg aatacaccaa caggcgggcc gcttccaatt
     1681 cggtctcgtt ttcggccatg cggtgcttga gcacctggaa cgacgccagc gggacgccga
     1741 attgcttgcg atccttcgtg tagcggatgg tatcgtcgag cgcggtgcgt gcgttgccca
     1801 cctgcgaagc cgcaatgctc agtcgctcgt attcgaggtg ctcgccgata tattgccagc
     1861 ccttgccggc ctcgccgagc agcgcatcct tgggaatgcg caggttatcc atgaagaatt
     1921 cggtggtgcc caagctcttg cgcacgatgg tattcatctt cttgatcttg agtcccggcg
     1981 tcgtgttcgg aaccaggaac acactgaagc cgtcgtgacg cccgggcccg gtgcgcgcca
     2041 gaatggcgat ggttgcgttg ggcaaatgcg ccccggagca ccataccttg ttgccgttga
     2101 gcacccactg atcgccgtcg agtaccgccg tggagcgcgc cgcggcggcg tcagaacctg
     2161 cttgcggctc gctgatcgag atcgaaaact tcgcgttgcc ggcgagaaac ggctcgaggt
     2221 agcggcgctt ctgctccgcg gttccgtggc gcgagatatt ggtcgcggtg aacatcgagg
     2281 tcatgatgca cgccgcggta tcgagactga acttgccgat cgcctcgcag aacaaggcaa
     2341 acatgatcgg atcagcggca ctgccgccgt cctgctcgtc caccagcagc ccaagccaac
     2401 cctgctgggc catcttcttg aatacttcct cgggaaactc ccggctggcg tcatgttcgc
     2461 gcgtgtactc gcgaccgacc tccttgtcca tgaagccatg gaccgtgtca ccccacaggc
     2521 gctgctcatg cgtgaactga aagtccatag gtccccttcg gcggtctgtt tagagagttg
     2581 aatacataat caaaggaata cacgattcgc cggtgcaatg gggtcatgat tgacaccacc
     2641 gtggatctgc gagtaaattt aaaacaaatg tttgttatag tcaatgaatc gcccttctcg
     2701 gatcgcccac acaggccgca ctgaatgcca actccgctgc agcgccgcgc ggcgaccgag
     2761 gcccgccacc ccgtgtggcg ggcaaaaacc cttgcacagg cgcttgactc ggctgccgcg
     2821 gagtttgccg ggcggccgct cgtcatcacc gactcccgaa tctacaccta tcgcgacatg
     2881 cagaactggt cgcgcacgct cgccgcaggc ctcatcgagt gcggcgtgcg cgcgggcgac
     2941 cacgtagcgt tgctactggc caattttccc gagttcgtcg caatcaaata cgccattgct
     3001 cgcgccggcg cagtcgcagt gccggtgaat tttgcattgc ggcgccagga gctcagttac
     3061 atcctcgacc aatcggacag caagctgttg ataaccatgg accggttgcg cgatcgcgat
     3121 tacctcgagg acctcgacgc tctcgtcgcc ggctgggaaa cttcaggcgg cggcgctcgc
     3181 tggcccaacc tgcgccgcgt cgtcgtacac gcaaccgagg gcgcgcagcg aagtcgtgcg
     3241 acctcgctcg atgagctcaa cgcgatggct acgccgtcga gccatgcaga acttgctcga
     3301 cgcgagggtg cggccgaccc gcatttccgt tcggacgtga tttatacctc gggaaccacc
     3361 ggattaccca agggggtcat gctcactcac gacatgattc tgcgcgcagc ctacgcttcg
     3421 gcctatacgc gcgccttcga ggatggccgt cgcatcctgt tcgcgttgcc catgtaccat
     3481 gtcttcggct tcgtggagtg cttgatcgca tgcacattcg tgggcggtgc catcgttccg
     3541 cagctgtcct tcgatgcccg caggatgctc gacgcagcgc gctcgcatcg agccaccgag
     3601 atcgtgtgcg taccgatgat gacgctcaag ctgttggatg aggcgcgcca gaacggtttc
     3661 gacggcgccg atttgctggc gatgttcaat tcgggcggcg cctcgcccgc cagcatttgg
     3721 caggacattc gcgaacttct cggcgcacgc gagatcgtga ccgcctacgg catgaccgag
     3781 accacggctt cgacgacctg cacgttgcct gagggtcccg acgaattctt gctgacgacc
     3841 aacggccgcc tcaagttcgc cggcgttgcc ggtgatgccg acctcggcgg tgtgctggcg
     3901 caatacaaga ccgtcgatcc ggcgaccggc gtcgacctgc cgttcggatt gcccggcgaa
     3961 ctggtcgctc gcggtccgat cgtgacccgc ggctattaca agaaagaggc ggaaacccgc
     4021 gccgcgttca acgccgatgg ctggctgcac accggagatg tcggcgtgat cactgctgac
     4081 ggatacctga cgctgaccgg acgtctgaag gaaacctacc gttgcggcgg cgaaatggtc
     4141 atgccgcgcg agatcgagga tttgttgaac gagcatcctc tggtcgcgca ggccttggtc
     4201 gtcgggctcc cagacgtgaa aatgggcgaa gtcggttgcg cctgcatcgt gcccgccggc
     4261 gacgtatttc ccgagcccca ggagctcatc gagctttgcg cgagccggct cgcgcgcttc
     4321 aaagtgccgc gacacgtgat atttgtgacg gccgcggagc tgccgctcac ggcgacgggc
     4381 agagcgcaaa aattcaagct cgctgatctt gcgaagcagc gcttgtcgat cgtatgagga
     4441 gtcaaccatg gtgatcgaac gtgcggattt tgcggtgatt gcggctcggg aaaccgagtt
     4501 cgagagcgtc atggagcgcg gctgcgcgct gctcgcgggc gcatcgggtt gcacatccgt
     4561 tgcgctgcta cggtgcgtcg agagaccctc gcgctactgc ctgcagctcg gctggaattc
     4621 gattgccgac caccacgcat tcaccaaaac gcaggacttt caaacattcc gggaaattgc
     4681 cggaccgttc ttcgccgagc ggcccgcaat ggaacatttt cagcctattc atcagcgggg
     4741 atgagtgaac cgtccatgcc gcacatcgtg accgataaat gccagcaatg ccgattcacc
     4801 gactgcgtaa ccacctgtcc ggtcgcctgc tttcacggcg actcgcagat gctgtatatc
     4861 gacaatgtcc aatgcgtgga ctgcggcgcg tgcgtaccgg cctgtccggt gcatgcgatc
     4921 tacgaacaat cggaattgcc cgccgacaaa ctggagtggc tcgccatcaa cgcgcagcgc
     4981 gcgccgggcc tgcccgtgat cgaagataag caagttccgc tgcccaccgc cgaggcacgc
     5041 aagcgtgaac tcggatactg acgccggcgc tttggggtcg gccgagaatc ctctgcacgt
     5101 cgcgatcgtc ggcagcgggc cgagcggctt ctacgcggcc gaagcattgc tcaagtcatc
     5161 cctgaccgtg cgagtggaca tgatcgagcg gctgcccgtg ccgttcgggt tggttcgcta
     5221 cggcgtcgct ccggatcacc ctcgactcaa gcaacccacg ttggtcttcg acaagatcgc
     5281 gcaatcgccc ggcttcacct tcctggggaa tgtcacggtt ggacgagaca tcagcttgga
     5341 acagttgcag agcacgcatc atgcagtgtt gttcgcctgc ggtgccgaat cggaccggcg
     5401 gctcggactc gcgaatgagg acctgcccgg cagtcacacc gcgactgaat tcgtcggctg
     5461 gtacaacggt catccggact actgcgagcg cacattcgac ctgagcagcg agaccgcggt
     5521 gattatcggt cagggcaatg tcgccatcga tgtggcgcgg atacttggca agtcggtcga
     5581 cgaacttcgc agtacggata tggccgagca cgccctcgat gcattagccc atagccgggt
     5641 acgcgatatt catatcatcg gtcgccgcag ccccgcacag gcgagcttca cgttcaagga
     5701 actgcgcgaa ctcggcgaat tgcgcggctg cgttacgcgc gtggccgcgg aggatctgca
     5761 gctgaatccg gcgagcctcg cggaacttga ggacaagacc aacttcaatg cggccaagaa
     5821 cgtcgaaatt cttcaaagct ggacgctgca cggcgccgcg accgcggaca agcgcatctg
     5881 gtttcatttc ctgcaaagtc cggtggccct gtcggggaag cagcgcctcg agagagtaac
     5941 gctggagaag aattccctcg ctggtccgcc atttggacaa accgcacaag gcacgggagt
     6001 tagtctcgat ctcagttgtg gtctgctgtt tcgcagcatc ggctaccggg gtctgccgct
     6061 gccgggggtg ccgttcgacg caaccaaggg agtctttccc aataccgcgg gacggataag
     6121 cgacacgctg ggtctgtatg ccgcgggatg gatcaagcgc ggaccctcgg gaatcatcgg
     6181 caccaatcgg gccgatgccg tggcttcggt gcaaagcctg ctggccgacg tgccgcgcct
     6241 cgactcgcgc ccgaaatcgg gtagcgaagg cgtgtatgcg gccttggctc ggatcgaatc
     6301 gcccgtgttg agctacggcg attggcggcg catcgatgcc gaagagcagc ggcgcggcga
     6361 agccatcggc aagccgcgag agaagatcac ccgagtgagc gatatgctgg ctttggcagc
     6421 gagtcccctc gcggagactg cgctcgagca cgcccaccac agttgaacac aggagtattg
     6481 tcatggcagg cagattacaa ggcaaagtgg ctttggtgac gggcacgggt tcgggaatcg
     6541 gcagggcgat tgccgaacga ttcatcgccg aaggcgccca agtcgtcgcg gtcgacatct
     6601 ccggcaatca gaatgcagtg gccgaaaaac tcggccccaa ctgcgtccca tttcaggccg
     6661 acgtgtccaa gtccgcggac gtgcaggcca tgctcgcgct cgccgcgtcc aaattcggcg
     6721 ctctgcacgt gctatgcaac aacgccgcca tcgaagggca tatggcaccc gtcggcgaat
     6781 atcccgaaga ggaatgggaa aaagtcatgg ccatcaatct gcgcagcgtc ttcctcggca
     6841 tgcgctacgg aattccattg atgctgaagt ccggcggcgg ttcgatcgtc aataccgcat
     6901 cgatggcggc gatggtggcc ttcccgaaca tggcggccta ttgcgccgcc aagggcggtg
     6961 tgaagcaatt gaccaagact gcggccgtgg aatacgcggc acagggcatc cgtgcgaacg
     7021 caatctgtcc gggaacgatc aagaccgcga taacagccgc gctgcccgcg gaatacatca
     7081 aggcgatcat caacgcgaat ccggtcaagc ggatcgccga gccggtggaa gttgcccatc
     7141 ttgcggtgtt cttggcaagc gacgagtcat cgttcatcac cggtaccgaa atcaccattg
     7201 acggcgggta cacggctctt tgattccggc ttggacatga ggttatgcaa tctaggtatg
     7261 gatacccgct gttaagctga ggatctgccg gctataaatg cctctctacc tgaactcgga
     7321 gagtgcggaa catgcgcgcg cgattgctac tggcagcttc gctctcatcg gtcgtactct
     7381 gcattaccgg ctgtgaatcg acgcctcaga ccgccaattc gtcggccaaa ccgctaccag
     7441 ccggggcgtt tgatccgcgg gatttcacgg gaatctggat gcagactccg gacaaaccct
     7501 tcaagaccta tcccctcact ccgcaatacg cggcgattct caaacaacgc cgcgacgatg
     7561 aagccgcggg aaagccgttt caagttgcgc aaaaccgctg cctccccgcc ggcctcgccg
     7621 catcgatgac gacgggcgcc tacccgctgg agatcttcta ccagaaaggc ggtcatgaga
     7681 tccttttcca aaaagagaac gtgggcgcac tctatcgggt ttatttgaat cgtccgcaca
     7741 agagcgccga tcaactatac ccgcagtttt atggagattc tattggacac tgggagggca
     7801 atacgttggt catcgatagc gtgagcttgg gcgcgacctc ggcgttcgat ctcatcacgc
     7861 cgcacagcga tgcattgcat gtcattcaac gcctgcagcg cccaacctac gacacgttgc
     7921 aggacgaaat cacctttgac gatcccaagg cgctgacaac accgatcacc gccaccgcgg
     7981 tgttcaagct gcacgcagac tgggagatac aggaatatga atgcaccaat gaacgtctga
     8041 tattcgatga gcatggcaat cagaccatat ctaaaagcac accccgatga tccgcccctc
     8101 aatttgtctg ctgctactgc tcgctgtcgt ctctcccgcc ttcgctcacc acagctacgc
     8161 aatgttcgat gcgaacagtc tcatcactcg agatgcgacg gtgaaagaat ttcagtggac
     8221 caatccgcac agttggctgc aggtgatgat gttggacgac aagggtgtcg tcgaggaatg
     8281 gagcttggaa ttgggccctt tggtcgttct gagtcgttgg ggctggaagc cgcattcact
     8341 gaatcccggc gacaaagtca aagtgagcct caatccgctg cgcgacggaa ctcacggcgg
     8401 aagactgatg agcatcgtgc tgccggacgg cgccaccttg agcggccagg gtggcgcaca
     8461 ggcgagcgct tcgagtgtgc cggcgagtca ttgaaggcgc ccaagcgcga gcgctctaat
     8521 tcacggtgcg gtggatctcc gtgttttgag ccgcggcaat cagccatccg gcatcctccc
     8581 gggtaagcac aaacagcaac acaccgtacc gtgggtcgcg cgttcgcgaa tcgccgagca
     8641 tttcccagtt cacgtgagcg acggccactt ccttgcacag gaaccgtaca gtgatcggct
     8701 cgaacttcaa cgtgctgtcc ttgaaaattg cccagtaagg gcgatcctgt ttggtgaatc
     8761 cgggagaatc tgccggaatc acaccgtgaa tgtaggcgtg ctgcgtctgt atgagcttcc
     8821 ctcctttcca aaagatacct gcgacgttga cgaaatcggc gtccgtcgca aacagttttc
     8881 cgaaggcatt caaatcgtgg cggttccacg tcgtggcgaa cccggtgacc acggtacgca
     8941 cctcctccgt gtcgttatgc gccgccacaa cgcggttagc gaacggcgaa atcagcacgg
     9001 ttacggcagc gagcacggtc gagagcttat gcatggcggt tgtcataggg ggatcctcga
     9061 gtggtcgtta atgccagtct aagccaactc ggccccatcc tggtcgcgcg tcgagtttgg
     9121 tatatccata ggtcgtgatg atagttcctg gcgatgcagc cgcacattag cctgcacaca
     9181 cctgcatacg gaacctctcg atgacttttc aacagttgcg gtatctcgtg gccgtgcatg
     9241 agaacgggct caacatcacg actgcggcgc gagagctgcg catttcccaa ccgggtgtga
     9301 gccggcagct caagctgatg gagaccgaac tcggttttga cttgttcgag cgcgaaggcc
     9361 gcgccttggt ccgaaccacg tcagcaggcg aggacatcgt ggcgcgcgcc acgacgatat
     9421 tgcgcgaatt gcaaaatgtg cagcgcacct cggcggagct gagccgggtg gacggtggtt
     9481 cgttgtcgat tgcgaccacc cacacccaag ctcgatacgt gctgccgcac gtggtgcgtg
     9541 cttttcgcgc aaaatatccg aaagttcgct tgcatctgca ccaaggcaca tccgaacaaa
     9601 tcgcagagat ggtcgcgcgc gaccgcgtcg atttcgcgat tgccacgggc tccgaggagt
     9661 tatttccgca cttgatccga ctccccgtgt accgctggca tcgcacggtc gtggttccgc
     9721 acggacaccc attggcgtcc aagggaaagc tgacgctcaa gaagctcgct gactacccga
     9781 tcgtgaccta cgtcttcagc ttctccggcc gctcctcctt gccggctcta ttcgagaccg
     9841 cggggctttc actcgatgtt gccctgaccg cacgcgactc ggacatcatc aagacttacg
     9901 tccgcatcgg tttgggagtc ggcatcttgg cgaaactcgc cgtcgatccc gtcatcgact
     9961 cggatctcgt cacgctcgat gccgctcatc tgttcgaagg acataccacc tggatcgggt
    10021 ttcgtcgcgg cgcgctgctg cgcgcttata tgtatgagtt catagagctt ctcgcatcgc
    10081 acttgccgcg aaagctggtg cgcgcggccg agaaaatgga tacgcaagag gacgtagacc
    10141 gaatattgac cggcttcaaa gttccactac gcgacaatac ttgagatttt ctccggctat
    10201 tgcgcagcac gcgccgctga atcggacttt taatttgcac cggccgccgc gtagcggcga
    10261 aaaaagctgt cctgcttcca cgccggccga accggatcat cggcaaccgc gatcgtcaag
    10321 gcgcggataa cttcctcgta gccggccgcg gtcgcaagat tgtgcggctg attcccatca
    10381 tccgaaggag catggtagcg atcgttgtgc caatccttga ggattttttc ctcgccggga
    10441 tcgctggcac cgacgtcgat catgaccgaa ggtatgccgt ggcggatgaa attgtactga
    10501 tcgctgcgaa taaaaatatt gtgcagcggc gccggatccg gttgcacacg aagaccttgg
    10561 tcggcggcta tcttcgcgac gcgatcgcca agatcggact ctgccaggcc gtaaacggtc
    10621 aacaccttca gcgggatgat cggcagaaac atgtcagtat tgatgtcggc gatcatcgat
    10681 gtggccggca cggtcggatg ggcggcgaag aacttcgatc cgagcaggcc tttctcctcg
    10741 ccggtcaccc agacgaagag cacggatcgc ttcagaggaa catgagctcg cttcagcgag
    10801 cgtgcgacgt cgagcagcaa tgcgcttccc gacccgttgt ccatggcacc gttgtaaatt
    10861 cggtcaccat tgattggtgc gcccacgccg acatgatcga tgtgcgccga gagcacgacg
    10921 tattcggctt tgagcttcgg gtcctttccc ggcatgcggg cgacgatgtt ggtcgactga
    10981 agatcgcgcg tgctcgtgtg cgtgacggcg ctcactgaag cggtcaacgg aaagcgcggc
    11041 aagactttcc gatccttcgc gagcgcagcg atctcggcga acgtatggcc ggtcccctcg
    11101 aggagcatgt cggcatgagc gggattgaaa atgacgccca gttggcagcc ggaggtctca
    11161 tcgaactccg ttccgaccaa gttcatgctg ggcagattgc ggttgagcgc catgcgcgcc
    11221 cacgacattt ccatcgacgc gggattaggc aaggaaatga cgccgatcgc cccggcggcc
    11281 ttgagcgcct tccaccgttc ggcgcgcgac tgatagtgcg cacttagcgc cgatggaacc
    11341 tcagccggcg agccggtgac aacgaccgcg accttgcctt tcagatcgag cccgaggaaa
    11401 tcgttatagg cctgctccgg aatgtttaaa ccgtagccga cgaacacgag gggagcatcg
    11461 accttcggcg cctgcgcata gcgagtgctg aaataagcct gctccccaag cgtcagcggt
    11521 tgcgcacggc cgccacgcac cagcgcgagg ctcgaacttg gctcatcgag ctccagagtt
    11581 tgcaacttga ccgactggta atagccgttg gacccggccg gctcgagccc gagcgtcttc
    11641 aattgctgca ccacgtaagc ttgcgcttgc cgctcgcccg tgctgccggt atcgcggcct
    11701 tcgaacttat cgtcggcgag tatttttacg gtactccacc agctctcacc gtcgaagtgg
    11761 tccgcggccc gcgccgcgga cgccaggacg agaaatacac tcaaggcata gttgcgaaac
    11821 atccgatgtt cctttcgttt agccgaagac cccgtactct acttgcaaga tttcgcccgt
    11881 gctggtgttg accaatatcg catcggtatc gtcgcgcacc cattcatagc cggccggggg
    11941 gacctcgagg gcgaacaacc agtaatcggc caacagaaac gatgcacccc aataagcgcg
    12001 cggcagaatt tgcccataag cccagcgatg ggccacccaa cccgcgggtc gatgataagg
    12061 gccgatatgg aagctacgtg ccgcctggaa attatgctga tacgccccgc gatccggcgc
    12121 cgcgggtctc gcatcccaac catggggctc ggttacgcgc tgataaccgc tcggacccgg
    12181 gtgaggttca ccatgagcgg gcggacgccc tccaccgccg cggtgctcgg gttgcgctag
    12241 tgcgccgctt tggatgagaa acgaccccat cgttacgacc gtcgcaagag ctgacaattt
    12301 gaccagtgga tatctagaga tcatgaggta ctcccgtgaa agaggaaatc agtcggtatg
    12361 ttcatggcgg gcattttgct gcgtttcatg ctacgcgcca agcgcaaggt gcgattctta
    12421 atcatccgta ctatccgtac tgcctaagag gcatgagctc ttacggttcc ggtatttggc
    12481 aagtcgaccg gccgggtcct aggatgaggc gagtatccac aattgccggc tacgccgcca
    12541 ggagtcctcg atgc
//
LOCUS       DASWZT010000184         3750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_93319, whole genome shotgun sequence.
ACCESSION   DASWZT010000184 DASWZT010000000
VERSION     DASWZT010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93319"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VGE92_04895"
     CDS             complement(<1..542)
                     /locus_tag="VGE92_04895"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEY1313188.1"
                     /translation="MSRIDALIGDMSLAEKLGQLTMTASSYAVTGPVIAGDSTDAIKA
                     GTIGNLLNMVGAEHVREMQRLAVEESRLKIPLLIGFDIVHGHRTLFPIPLGEAALFDP
                     EAWALSARESAKEAAADGLAMTFAPMLDVARDPRWGRSAEGPGEDTHVAVRVAQAKVR
                     GFQGANLAAPDALAAVAKHF"
     gene            611..2377
                     /locus_tag="VGE92_04900"
     CDS             611..2377
                     /locus_tag="VGE92_04900"
                     /inference="COORDINATES: protein motif:HMM:NF017775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEY1313189.1"
                     /translation="MKSESTPEEIANSGLVPQLGTMIGALWAAPVRNTLFVLSGALFI
                     DIAATAYGQNRLNTWNQPFYDALSHRDYAEFLAQLRVFGVIAGTLLILNVAQRWLTET
                     LKLRLRQGLARDLIQNWLQKGRAFRLANAGAIGVNPDQRMHEDARHLTELSADLGVGL
                     LQSSILLCTFVAVLWSLSSNFAFHIGARLVPIPGYMVWAAVIYSGSASLLSYWVGRSL
                     VERNAQRYAREADMRFSLVRVNEHIDAIALAGGEADEERRIEVDVAAVLAATGRLVTG
                     LTNLTWVTSGYGWFTLVAPILAAAPLYFAGNISFGGLMLAAGAFNQVQSSLRWFVDNF
                     STIADWRATLLRVASFRRAVIDTDVLHDVESRIGFVDGDPGKFSIDDLEIASAAGATM
                     LKEKKIDIDAGERVLIVGESGTGKTLLFRALAGLWPWGAGRVVRPRGEEILYMPRTPY
                     LPPGTLREVLAYPSAIDAFEPSAYAAALHRLHLDRLTAKLDAHERWDRELSDDEQQSL
                     AFARVVLHAPPWLLIDEVLDALDADVRQCILDILAQDLTHTGVIHIGRADADAQHFAR
                     VLHLVKDPNLRRLAAQAAPVAA"
     gene            complement(2387..3391)
                     /locus_tag="VGE92_04905"
     CDS             complement(2387..3391)
                     /locus_tag="VGE92_04905"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEY1313190.1"
                     /translation="MQPLHLTYLNGPDVARLELSDREILDAVEAALAAQGRGAVVIEP
                     RVHLVPQSSSIGHFNVLRGVVQPLNVAGVKVVGDFVDNYRLGLPSELALLALFDPATG
                     APKAILDATSLTDMRTGAMSALGAKFLARSNSRVLAHIGARGTAYWNVRLLDHLFDFA
                     EIRVHSQRPQSRLDFAQRLSRDLAKPVRAVDSWQSCVDGADIVVEASRLAAPEPLLKT
                     EWIKPGALVMPYGTMSAVQLSLTDIMDKVVVDDWGQCRKGLPFGALRAHVDSDRITEK
                     SLHAELGQIVAGTKPGRESDAETILFWHRGLSLSDIALGDAMLKKAAAMGIGQTLRFA
                     "
     gene            complement(3400..>3750)
                     /locus_tag="VGE92_04910"
     CDS             complement(3400..>3750)
                     /locus_tag="VGE92_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313191.1"
                     /translation="AMHSLREAGAWCHEGQLQSIDTSEFPDDVRAKLVTSKLELPLRY
                     LEYRRLIKYKRNSNATLTIGVTFAGADRAIRLHTRRGRADLWFRDNKDGVFGFITTVF
                     VAFVTALVVVLLMR"
BASE COUNT          650 a         1190 c         1174 g          736 t
ORIGIN      
        1 caaaaatgct tggcgaccgc cgcgagtgca tctggcgccg cgagattggc gccttgaaac
       61 ccgcgcactt ttgcttgcgc gacgcgtacg gcgacgtggg tatcttcgcc cggtccctcg
      121 gcgcttcgtc cccagcgcgg atcgcgggct acatccagca tcggcgcgaa ggtcatggcc
      181 aagccgtcgg ccgcggcttc cttcgcggat tcgcgcgcgc tgagtgccca ggcctcggga
      241 tcgaacagcg ctgcttcacc taacgggatc ggaaacaacg tgcgatgccc gtgcacgatg
      301 tcgaatccga tcagcaacgg tattttgaga cgtgattcct ccaccgccag tcgctgcatc
      361 tcgcgcacat gctcggcgcc gaccatattg aggaggttgc cgatggtgcc cgccttgatg
      421 gcatcggtcg aatcgccggc gatgaccggt ccggtaaccg cgtagctgga ggcggtcatg
      481 gtgagttgcc cgagcttctc ggccaagctc atgtcgccga tcaaagcatc gatccggctc
      541 atttaattgc gctccacgac ggggcattac ctgataatgg cgtgatcaca gtgctccaga
      601 gatgcaatcc atgaaatccg aatcgactcc cgaagagatt gccaattccg gcctcgtgcc
      661 gcaactcggc accatgatag gtgccttatg ggccgccccg gtacgcaata cgctgttcgt
      721 gctctccggc gctcttttca tcgacatcgc ggcgaccgcc tatggacaga atcgcctcaa
      781 tacctggaac caacccttct atgatgcgct gtcgcatcgc gactatgcgg aattcttggc
      841 gcagctgcgg gtgttcggcg tgatagccgg gacgctgctg atattgaacg tcgcgcagcg
      901 ctggctcacc gagacgctga agttgcgctt gcgccaagga ttggcgcgcg atttgattca
      961 aaattggttg cagaagggcc gcgctttccg gctcgccaat gcgggcgcga tcggcgtgaa
     1021 cccagatcag cgcatgcacg aggatgcacg ccatctcacc gagctgtcgg ccgatctcgg
     1081 ggttggcctg ctgcaatcct cgattctgtt gtgcactttc gtcgcggtcc tgtggtcttt
     1141 gtcgagcaat ttcgcgttcc atatcggcgc ccggttagtt ccgattccgg gctatatggt
     1201 gtgggccgcc gtcatctatt cgggatccgc ttccctgttg agctattggg tgggccgaag
     1261 tctcgtcgag cgcaacgcgc agcgctatgc ccgcgaagcc gacatgcgct tctcgttggt
     1321 gcgcgtcaac gagcacatcg acgccattgc gctcgccgga ggcgaagcgg atgaggagcg
     1381 gcgcatcgag gtcgacgtcg cggccgtgct ggctgccacc ggccggttgg tcaccggcct
     1441 caccaatctg acgtgggtca cctccggcta tggctggttt acgctggtcg caccgatctt
     1501 ggcggcggcg cctctgtatt ttgcgggaaa tatttcattc ggcggcctga tgctcgccgc
     1561 cggcgcattc aatcaggtgc aatcctcttt gcgctggttc gtggataact tcagcaccat
     1621 cgccgactgg cgcgcaacct tgttgcgcgt cgcaagcttt cgccgcgcgg tgatcgatac
     1681 cgatgtgctg catgatgtgg agagccggat cggctttgtc gacggcgacc cgggcaagtt
     1741 cagcatcgac gatttggaga tcgcctcggc ggcgggcgcc accatgctca aggagaagaa
     1801 aatcgacatc gatgccggcg aacgggtatt gatcgtcggc gaatccggca cgggaaagac
     1861 gttgctgttt cgcgcgctcg ccggtttgtg gccctggggc gccgggcgtg tcgtccgtcc
     1921 gcgcggcgag gagatactgt atatgccgcg cactccctac ctgcctcccg gtaccctgcg
     1981 cgaagtgctc gcctacccct cggcgatcga tgcgttcgag ccgtccgcct atgcggcagc
     2041 gctgcaccgc ctgcacctcg atcggctgac agccaaactc gatgcgcacg aacgctggga
     2101 tcgggaattg agcgatgacg aacagcagtc gctggctttc gcgcgcgtcg tgctgcacgc
     2161 accgccgtgg ctgctcatcg atgaagtcct cgatgcattg gatgcggatg tccggcaatg
     2221 cattctcgac attctcgccc aggacctgac gcacaccggc gtcatccaca tcggccgcgc
     2281 cgatgccgat gcgcagcatt tcgcgcgcgt tctgcatctg gtcaaagatc cgaacctgcg
     2341 cagactcgct gcgcaagccg cgccggtcgc tgcctagcgc ggctgctcag gcgaaacgca
     2401 gcgtctggcc gatgcccatg gcggcggcct tcttcagcat ggcatcgccg agcgcaatgt
     2461 cgctcaaact caagcctcgg tgccagaata ggatggtttc ggcgtcactt tcgcggccgg
     2521 gcttcgtgcc cgcgacgatc tgcccgagct ccgcgtgcag gctcttctcg gtgatcctgt
     2581 ccgaatcaac gtgcgcgcgc aatgcgccga agggcagccc tttgcggcac tgcccccaat
     2641 catcgaccac gaccttgtcc atgatgtcgg tgagcgagag ctgcaccgcg ctcatcgtac
     2701 cgtagggcat gacgagtgcg cccggcttga tccattcggt cttcagcagc ggctcaggcg
     2761 cggcgagacg cgacgcctcg accacgatgt cggcgccgtc cacgcagctc tgccagctgt
     2821 cgaccgcgcg caccggctta gccaaatccc gcgacaggcg ctgcgcgaag tcgagccggc
     2881 tctgcggccg ctgcgaatgc acgcggattt ccgcgaagtc gaacaagtgg tccagaagcc
     2941 gcacgttcca atatgcggtg ccgcgcgccc cgatatgcgc gagcaccctg ctgttgctgc
     3001 gcgccagaaa cttggcgccg agcgcgctca ttgcgccggt gcgcatgtcg gtgagcgagg
     3061 tagcatcgag aatcgctttg ggtgcgccgg tagccggatc gaacagagcg aggagcgcga
     3121 gttcggaggg caacccgagc cggtaattat cgacgaaatc gccgaccacc ttcactccgg
     3181 caacattgag tggctgcacg acgccgcgta gaacattgaa gtgcccgatg ctggaggact
     3241 gcggcaccag atgcacgcgc ggctcgatga ccacggcgcc gcgtccttgt gcagcgagcg
     3301 ctgcttcgac cgcatcgagg atctcgcggt cgctgagttc gaggcgcgcc acatccgggc
     3361 cgttgaggta ggtcagatgg agaggctgca ttgcgcgatt tatctcatca gcagcaccac
     3421 gaccagcgcc gtgacaaagg caacgaatac ggtggtgatg aaaccgaaga cgccgtcctt
     3481 gttgtcgcgg aaccataaat ccgcgcgtcc gcgccgcgtg tgcaggcgaa tggcgcgatc
     3541 cgcgccggcg aacgtcacgc cgatcgtgag ggtcgcattg ctgttgcgct tgtacttgat
     3601 cagccggcgg tattccaagt agcgcagcgg caattccagt ttcgaagtca cgagcttggc
     3661 ccgcacgtcg tccggaaatt ccgaggtgtc gatgctctgc agctggccct cgtggcacca
     3721 ggcaccggct tcgcgcagcg aatgcatcgc
//
LOCUS       DASWZT010000185        11030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_88348, whole genome shotgun sequence.
ACCESSION   DASWZT010000185 DASWZT010000000
VERSION     DASWZT010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11030
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88348"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="VGE92_04915"
     CDS             <1..1055
                     /locus_tag="VGE92_04915"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEY1313192.1"
                     /translation="KVAILLTTPNAVVAREAPIARQKLNELILNFRKALGNPALDPEP
                     QARVLYQLLIEPIAADLHQAGAKTLMLDLDDTLRYLPFAALYDGKNYLIETMSVVMVT
                     EAVRDKLSVQSNPDWKVWGLGITKGGPGYAALPYAGVELNGIAGQQGILAGQVMLDQA
                     FTENSLREGLELSYPIVHIASHFQFKPGSMDDSFLLLGDGRRMTLAEIRTKLNFSSVD
                     LLTLSACETALGDDSVAHHGVEVEGLGALAQEAGAKAVLATLWPVADESTAALMRDLY
                     LAHKVDHLDKAESLRQAQLALLHGTVKADAGSVQRRGLTRTADTAAGRPFKADPNAPF
                     AHPFYWAPFILMGNWL"
     gene            complement(1065..3125)
                     /locus_tag="VGE92_04920"
     CDS             complement(1065..3125)
                     /locus_tag="VGE92_04920"
                     /inference="COORDINATES: protein motif:HMM:NF020509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEY1313193.1"
                     /translation="MPEQPPVYWAFLSYSSHDRATAIWLQRALETYTVPRRLVGRSTP
                     AGPAPRRFRPIFRDRTELAASADLVARIANALAQSAYLIVVCSPNAAKSHWVDDEVER
                     FRALHGDARILTVLVGGSPESDHQDYFPPALRYRESAQARADRVEPIAADLRPSGDGR
                     RMARFKLLAGMLGVGLDELVRRDAQRRHRQLLVITASSVVGLVITGALATAALIARND
                     ARRQRAHAEGLIEFMLTDLRKKLEPAGRLDAMDGVGREALKYYEAQSPADLDAQSLAR
                     RARALRLMGEIRVQRGDLGQALEGFEQASATTQELLARRPSDGQVIFNHAQNVFWVGE
                     IAHQRGDIAKAEASFQHYRRLAEQLIAIDPANDDWRAEVGYAQSALGILFFEQGRVVE
                     AVAAFERALAIAEGLANRRPDDPNLQLELGQSHAWLADALQRSGRLVETRAHRDKELK
                     IYGSMLEKDPTLRQAKYSTIVALQRLGDLAKIEDDEKGALADFRESAVQGDALLAGEH
                     DNMDLAAVVAIGQIDLGESLLAAGQPDAALAAYQKADALLTAALAHDASVALWRDYRD
                     RAALLRAEILSAGGHRSEALQIDQEVLGRLETQRAIPNTEPFWLLERFRLQVGDDLAA
                     LGRPQDARPQWSAVVDNLPAPAEKYQPELLKLLSSAELRLGRTVEAQAVAKRLKQLSR
                     VPGS"
     gene            complement(3130..3768)
                     /locus_tag="VGE92_04925"
     CDS             complement(3130..3768)
                     /locus_tag="VGE92_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313194.1"
                     /translation="MKREFTSFWLLTSVMIALVLSSCGPGPAGQTQQQSNASSRVSGP
                     DFATDCASATQLDVVAVLYKGTDTYTEIPLFKLCPTNASNSAGTVQLDKQGNLTLNPK
                     DTPYSSIHFYLDASLTRHAWKSDPNQSVWMADDIVKPTAANWPFCLGSKNVKSAGTAE
                     IFFSLCSTMGETTYQYTLHLDQKSPTDNVTVDVGIDPQIINKPHSIETPSKP"
     gene            complement(3873..4910)
                     /locus_tag="VGE92_04930"
     CDS             complement(3873..4910)
                     /locus_tag="VGE92_04930"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY1313195.1"
                     /translation="MTRPESDSNDARLLERYRKASDADPVAPTQAVRAAILAEARRVA
                     EQAAAAPPPVFDTSQPAANQPRWRMAAFGTLGAALLGALIVAPRLWERGPAPQSTAAN
                     QAAPATSAPAANAPLADAPVNVPAPDALAPNGPAHIATARNAAAPKAAPNAAPAAAEP
                     ASPPPVASLQAPSESQLAEADSGSSAQRAGSAAATPDRTAALIAQRQAAQESLAAPHY
                     TPDPRIADALSGRLSSFAVRSAAPASAGARAKAGASAASLGAAAASGDSSAVERLLDQ
                     GATLDARDDLGRTPLMLATLNGQVDTVRILLARGADPNIADNEGHTALQVAQSAHRDE
                     IARLLQDAGAQ"
     gene            complement(4907..5476)
                     /locus_tag="VGE92_04935"
     CDS             complement(4907..5476)
                     /locus_tag="VGE92_04935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY1313196.1"
                     /translation="MEPSADSDEDLMLRFGRGDAAAFEALYRRHESRVFRYLLRNVRD
                     HAVANDLMQEVWFAVARSGQRYQPSAKFTTWLFTLAHHRMVDWIRGARPSQSLDADGE
                     GAALSETLAADPREEPLAAVASEDEAAVLLKAVSLLPAEQKSAFLLQAEGGLSLEEIA
                     AATGASFETVKSRLRYARAKLRQLLWEYA"
     gene            complement(5488..6363)
                     /locus_tag="VGE92_04940"
     CDS             complement(5488..6363)
                     /locus_tag="VGE92_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313197.1"
                     /translation="MKTACLGLVLALAALDANALGRLAEVQILDRDTGTVLPTYRYHG
                     EYWVAGTPGNRYSISIRNNRGDRLLAVTTVDGVNVITGETGAFGQSGYVYGGGETYSI
                     TGWRKSDQEVAAFEFTALPNSYAARTGRPANVGVVGVALFLERPAPPPPVTQYSPPIG
                     SLTEESRSSRFAASAGAARGAPAAPARQADAPTAPANEASAAQAVPAPAPLAKLGTGH
                     GAREASVVFSTQFDRLTNTPNEIIRIRYDSWENLVAQGVVQPRRPQPGEPNAFPDSAV
                     SQYVPDPPSNVPMRR"
     gene            complement(6467..7366)
                     /locus_tag="VGE92_04945"
     CDS             complement(6467..7366)
                     /locus_tag="VGE92_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY1313198.1"
                     /translation="MKRLTSILLIATALGAARLAAAAGPVPVEPAATPFQIGALQAFA
                     LADAKFVGANDGSVFGAAVGPAEVGKVLQAAGAPADSISLSVTALLVKDGKHVVLLDT
                     GLGPKVKGVLLQSLGKAGLAPGDVTDVLITHSHGDHIGGLVAADGQPAFPHATVHMSA
                     AEWAFMQSRQDAADVVKAVTGHVKTFEPGAIVVPGITAVAIQGHTPGHMGYEIVSGKD
                     RLLDIGDSAHSSILSLARPDWEMQFDTDKVLARQSREKLLRDLAKSHEQVFSPHFPYP
                     GVGQVVAEGDHFAWKATLPVAAP"
     gene            complement(7436..8668)
                     /locus_tag="VGE92_04950"
     CDS             complement(7436..8668)
                     /locus_tag="VGE92_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HEY1313199.1"
                     /translation="MNRHPSWLASIAAAAAFAASAAQAAPAAVDVAAADNAFGFRLLN
                     AVQKATPTGNVILSPVSAALDLAMVLNGADGQTRDEMRSALSLDGSELDAINAANAAL
                     IKTIRTPAKSVTLAVADSLWVDNRRATLKPEYLKQAQASYDAEMTDLDFSSPGAAARI
                     NDWASEHTHGKITQVIDRIDRADLALLLNAVYFKGEWTFKFDAAQTKLRDFNLAGGSV
                     KQLMRMAQSGRFEYFETPAMQAIRLPFGAGDLGLEVLLPSSTSSLAALEAQLTPQNWQ
                     AWKARFVRRPGNIELPRFELKSKYRLNAALQALGMNRAFHAGGADAAQLTSMFSPVPG
                     AAHDRNFFISAVLQSVYWKVDEEGSEAAAVTTTTMRATLAARPAEPFRMIVDRPFFCA
                     IEDGRSGTLLFVGAIYEP"
     gene            complement(8665..10398)
                     /locus_tag="VGE92_04955"
     CDS             complement(8665..10398)
                     /locus_tag="VGE92_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family serine peptidase"
                     /protein_id="HEY1313200.1"
                     /translation="MKPFNLAASAVTLAMIFGTISCSSSPPAPAGSGATAVPGAQPML
                     APHLPRSVADGTAAMTGPVGAQQILRPVIQLPLRNQAELTQLLHDLYDPKSRKFHQYL
                     SVSDFTARFAPTARDYAAVVAWAKRNGLKVIATTPNRRLVAVEGSVNTVNRAFHVTIN
                     NYRHPTEGRTFYSPNREPSTVGLTVPLLQITGLNNSVLPHPMFRHGVAAHLASGSGTS
                     GEFLPSDMRAAYYGNGPLTGAGQSIGIFSFDGYLASDVQLYYSKTGMTSSVPINNVLV
                     GGFNGACTSVSSAPSSTCDDGEQVLDIVNAIGMAPGIKQILFYEGTSNTQILNQMATD
                     NIAKVLSSSWGWNPADAASDDPIFQQFAAQGQTFVTASGDDGELNSSTYDFPAVDQYI
                     TQVGGTVLTTAGPGGAWSAETAWPQSGGGFISGTPIPSWQQLAGVINASNQGSTTLRN
                     SPDVAAEADFDNPTVSNGAFMTGYGGTSFATPRWAGFLALVNQQSVANGKGTVGFLNP
                     ALYNLGVSSGYSGALHDITSGSNPPTTGSGSGFNAVPGYDLVTGWGSPTGVGLINQLA
                     GGAASSQAARR"
     gene            complement(10753..>11030)
                     /locus_tag="VGE92_04960"
     CDS             complement(10753..>11030)
                     /locus_tag="VGE92_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313201.1"
                     /translation="GTSLSSPFWSAIIADRDGFWHDRIGNANPLLYRLYNVDYQGFFH
                     DITGRGPSNSDTIFPTNNGYFPATRGYDLATGIGTPKMGAIITGVAE"
BASE COUNT         1979 a         3473 c         3526 g         2052 t
ORIGIN      
        1 acaaggtcgc gatcctcctg accacgccca acgcggtggt ggcgcgagag gcgcccattg
       61 cacgtcagaa gctcaacgag ctgattttga atttccgcaa agccctgggc aatccggcac
      121 tggatcccga gcctcaggca agggtgctgt atcaattatt gatcgagccc atcgccgccg
      181 atttgcatca ggccggcgcc aagactctca tgctcgacct cgacgacaca ctgcgctacc
      241 tgccgttcgc ggcgctctac gacggcaaga attacctgat cgagacgatg tcggtggtga
      301 tggtgaccga ggccgtgcgc gacaagctga gcgtgcagtc caatccggat tggaaagtct
      361 gggggctcgg gattaccaag ggcgggcccg gctacgcggc gttgccgtac gcgggggttg
      421 aactcaacgg catcgccggt caacagggga ttcttgccgg ccaggtgatg ctcgaccagg
      481 cgttcaccga gaactcgctg cgcgagggac tcgaactgtc ctatccgatc gtgcacattg
      541 cgagccactt ccagttcaag ccgggcagca tggacgactc ctttctgttg ctgggggatg
      601 gccgccgtat gacgctcgcg gagatcagaa ccaaattgaa tttcagcagc gtggatctct
      661 tgacgctgtc ggcgtgcgag accgcgctcg gcgatgacag cgtggcccat cacggcgtcg
      721 aagttgaagg cttgggcgct ctcgctcagg aggccggagc gaaggcggtg ctggcgacgc
      781 tgtggccggt cgcggatgag agcacggctg cgttgatgcg cgacctgtat ctcgcgcaca
      841 aggtcgatca cctggacaag gccgagtcgt tgcgccaggc gcaactggcg ctgctgcacg
      901 gtacggtgaa agccgacgcg gggtcggtgc agcgccgcgg attgacgcgc accgccgaca
      961 ccgcggccgg gcgccccttc aaagctgatc cgaatgcgcc cttcgcgcac cctttttatt
     1021 gggcgccgtt cattctgatg gggaattggt tgtaacgact tgcctcacga gccggggact
     1081 cgcgacagct gtttgaggcg cttggcgacg gcttgtgctt ccaccgtgcg acccaatcgc
     1141 aattcggccg atgacaatag ctttagaagc tcgggctggt atttctcagc gggagccggg
     1201 aggttgtcca cgacggcgct ccattgcggg cgagcgtcct gcggacggcc gagtgccgcg
     1261 aggtcatcgc ccacctgtaa acggaatcgc tcgagaagcc agaagggttc ggtgtttgga
     1321 atcgctcgct gagtttcgag ccggccgagc acttcttggt cgatctgcag cgcctcggag
     1381 cgatgaccgc cggcagaaag gatctcggcc cgcagcagcg cggctctatc acggtagtcg
     1441 cgccagagag caacgctcgc gtcgtgggcg agcgcggccg tcaggagtgc atcggctttt
     1501 tggtatgccg caagagcggc atccggctgc cccgctgcga gcaaggactc gccgagatcg
     1561 atctgaccga tcgcgaccac agcagcgagg tccatattgt cgtgctcgcc ggccaagagg
     1621 gcgtcacctt gcaccgcgga ctcacggaag tccgcgagag cgcccttctc gtcgtcctcg
     1681 atcttcgcaa ggtcaccgag cctttgcagt gcgacgatcg tcgagtactt cgcctgtctg
     1741 agcgtcggat ccttctcgag catcgaccca tagatcttga gttccttgtc gcgatgggcg
     1801 cgcgtctcga cgaggcgccc gcttctttgc agtgcgtcgg cgagccaggc gtggctctga
     1861 cccaactcca gctgcaaatt aggatcgtcg ggacgacggt tcgccaatcc ctcggcgatc
     1921 gcgagcgcgc gttcgaaggc ggcgactgct tcaaccacgc ggccctgctc aaagaagaga
     1981 attcccagcg cgctctgtgc atagcccacc tccgcgcgcc aatcgtcatt ggcgggatcg
     2041 atcgcgatga gctgctcggc gagtctgcga tagtgctgga atgacgcctc tgctttcgcg
     2101 atgtcgccgc gctgatgcgc gatctcaccc acccagaaga cattctgggc gtgattgaaa
     2161 atcacctggc catcgcttgg gcgtcgggcc agcaactcct gcgtggtggc cgaggcctgc
     2221 tcgaaccctt ccagagcctg gccgaggtca ccgcgctgga ctctgatctc gcccatgagc
     2281 cgcagcgccc gcgcgcggcg cgccaatgac tgggcgtcga ggtcggcggg actttgcgct
     2341 tcataatact tgagcgcctc gcgcccgacc ccgtccatgg cgtccagccg accggccggc
     2401 tcgagcttct tgcgcagatc cgtgagcatg aattcgatca agccttcggc gtgcgcgcgc
     2461 tgccgacgcg catcattgcg cgcgatcaac gcggcggttg ccagagcacc ggtgatgacc
     2521 aggcccacca cggacgaggc ggtgatcacc agcaactgcc gatgccgacg ctgagcgtcg
     2581 cgtcgcacca gctcgtccaa gccgacgccc agcatgcccg ccaataactt gaaccgggcc
     2641 atgcgccgac cgtcgccgct tgggcgcaga tcagcggcga tgggttcgac tcgatcggct
     2701 cgggcctggg cggactcgcg ataacgcagc gccggcggga aatagtcttg atgatcgctc
     2761 tcaggcgaac cgcccacgag caccgtcaga atccgcgcgt cgccatgcag tgcgcggaag
     2821 cgctcgacct catcgtcgac ccaatgcgat ttcgccgcgt tcggcgaaca cacgacgatc
     2881 aaatacgcgg actgggcgag cgcattcgcg atgcgcgcga cgagatccgc gctcgccgcc
     2941 aactcggtgc gatcgcggaa gatcggccga aagcggcgcg gcgcgggtcc ggcaggagtt
     3001 gatcggccga cgagacggcg cggcaccgta taggtctcga gcgcccgctg cagccatatg
     3061 gccgtggctc gatcatggct gctgtaactg agaaaggccc agtaaacggg gggttgctca
     3121 ggcacaaatc tagggctttg acggcgtctc gatggagtgg ggcttgttga tgatttgggg
     3181 atcgatgcct acgtccacgg tgacgttgtc agtagggctc ttctggtcca aatgcaatgt
     3241 gtattggtac gtagtttccc ccatcgttga gcatagagaa aaaaagattt ccgctgttcc
     3301 cgcggatttg acatttttgc tgccgaggca gaacggccaa tttgcagccg ttggtttcac
     3361 tatatcgtcc gccatccata cggattgatt tgggtcgctt ttccaagcgt ggcgcgtaag
     3421 actcgcatcc aggtaaaaat gtatgctgga atacggtgta tctttcgggt tgagtgtcag
     3481 atttccttgc ttgtccaact gcacggtgcc ggcgctgtta ctggcatttg tcgggcagag
     3541 tttgaacaaa ggaatctcgg tgtacgtatc ggttcctttg tagaggacag ccactacgtc
     3601 gagctgtgta gcactagcgc aatcggtagc gaaatcgggg cccgaaacac gcgaggaagc
     3661 gtttgattgc tgctgagttt gacccgcggg gccaggaccg catgaactaa gcaccaaagc
     3721 aatcattacc gacgtcagca accagaacga cgtgaattcc cttttcattt tgcccttcca
     3781 agaggtgctt ttttcgtagt agacgggaac ggctgagcgt cgtcaagcgc ttcacggctt
     3841 gcgtcgcgct cgcatgtgcc atcgctatcg ggtcattgcg cgccggcgtc ttgcaacagc
     3901 cgcgcgattt cgtcgcggtg ggccgattgc gccacctgaa gtgcagtgtg tccctcgtta
     3961 tccgcaatat tcggatcggc accgcgggcg agcaagattc ttacggtgtc gacttgtcca
     4021 ttgagcgtcg ccagcatgag cggcgtgcgg ccgagatcat cgcgcgcatc gagcgttgcg
     4081 ccctgatcga gcagtctctc gacggcggac gagtctccgg atgcggccgc cgcccctagc
     4141 gaggccgcgg aagcgccggc tttcgcgcgt gctccggcac tcgccggcgc cgcggagcgc
     4201 acggcaaagg aactgagcct gcctgaaagc gcatccgcga tcctaggatc cggcgtgtag
     4261 tgcggggcgg ctaaggattc ctgagctgcc tggcgctgcg ctatcaatgc tgccgttcga
     4321 tctggtgtcg cggctgccga acccgcgcgt tgcgccgaac tcccggaatc cgcctcggcc
     4381 aattgcgact cgctcggtgc ctgcaggctt gctaccggag gtggcgaggc aggctcggcc
     4441 gcggccggcg ccgcgtttgg cgccgctttt ggcgctgcgg cattgcgcgc cgtagcaatg
     4501 tgcgccgggc cattgggcgc caaggcatcg ggtgccggga cattgactgg ggcatcggcc
     4561 agcggggcgt tggccgccgg ggcactcgtc gccggggccg cctgattggc cgccgtcgat
     4621 tgtggcgccg gcccacgttc ccacaaccgc ggcgccacga ttagcgcgcc caacagcgcc
     4681 gcacccaagg tcccgaaggc ggccattcgc caacgcggct gattggccgc gggctgcgag
     4741 gtgtcaaaca cgggtggcgg tgccgctgcc gcctgttcgg cgacgcgacg tgcttcggca
     4801 agaatcgcgg cgcgcacggc ctgcgtgggc gcaaccgggt cggcatcgct cgccttgcgg
     4861 tagcgttcca gcaaccgcgc atcgtttgaa tcggactcag ggcgcgtcat gcgtactccc
     4921 acagtaattg gcggagcttg gctcgcgcgt agcgcaatcg actcttgacc gtctcgaagc
     4981 tcgcgcccgt ggctgcggca atctcctcga gactcaggcc gccctcggct tgcagcaaaa
     5041 atgccgactt ctgctctgcg ggcaacaagc tcacggcctt cagcagcacg gctgcctcgt
     5101 cttcggaggc gaccgcagcc agcggctcct cgcgcggatc ggcagcgagt gtctcgctca
     5161 aggccgcgcc ctcgccgtcc gcgtccaagc tttgcgacgg gcgtgcgccg cgaatccaat
     5221 cgaccatgcg gtgatgcgcg agcgtaaaga gccatgtcgt gaacttggcg ctcggctgat
     5281 aacgctgccc actgcgggcg acggcgaacc agacttcctg catcaaatca ttggcaaccg
     5341 cgtgatcgcg cacgttgcgc aacagatatc gaaaaacgcg cgactcgtgg cgccgataca
     5401 gcgcctcgaa cgcggcggcg tcgccgcgtc cgaagcgcaa catgagatct tcatcgctat
     5461 ccgcgcttgg ctccattcac gccattatca gcgtctcatc ggcacattgc tcggcggatc
     5521 cggcacatat tgcgaaaccg cggaatccgg gaatgcgttc ggttcgccgg gttgcggcct
     5581 gcgcggctgc accaccccct gcgcgacgag gttctcccag ctgtcgtagc gaatccgaat
     5641 gatttcgttc ggcgtgttgg tcagccggtc gaactgggtc gagaagacca ccgacgcttc
     5701 gcgcgcaccg tgtccggtgc caagcttcgc caggggtgcg ggcgccggca ctgcctgcgc
     5761 cgccgaagct tcatttgccg gtgcagtcgg cgcgtcggct tgacgggcag gcgccgcagg
     5821 ggcgccgcgc gcggcgccag cgctcgcggc gaaacgcgag gagcgtgact cttccgtcaa
     5881 ggacccgatc ggcggcgaat actgagtgac cggcggcggc ggcgcgggcc gttcgagaaa
     5941 caaggcgacg ccgaccacac cgacgttggc cggccgcccg gtgcgcgcgg cgtaggagtt
     6001 cggcagcgcc gtaaattcga aggccgcgac ttcctggtcg ctcttgcgcc agccggtaat
     6061 cgagtaagtc tcaccgcccc cataaacata gccgctttgg ccgaaggcgc cggtctcgcc
     6121 ggtgatgaca ttgactccat cgacagtggt cacggcgagc aagcggtcgc cgcgattatt
     6181 gcgaatcgaa atcgagtagc gattgcccgg cgtgccggca acccagtatt cgccatgata
     6241 gcgataggtc ggcagcacgg ttcccgtgtc gcggtccaag atttgaacct cggccaagcg
     6301 gcccaaggcg tttgcatcga gcgcggccaa tgcgagaaca aggcccagac aagcggtctt
     6361 catatcgact cccggtgatt tgaactaccg gttgaatcga tacggatccg gaaatggggt
     6421 taaaccgaca gtggccggat tatgtccaac cgttttcgcc ccgcgcctac ggtgcggcga
     6481 cgggcagcgt tgctttccag gcgaagtgat cgccctcggc cacgacctgg ccgacgccgg
     6541 gataggggaa atgcggcgaa aagacctgtt catggctttt cgccaaatcg cgcaagagtt
     6601 tttcccggct ttgcctggcg aggaccttgt ccgtatcgaa ctgcatctcc cagtccggcc
     6661 tcgccagcga caagatggag ctgtgcgcgg aatcgccaat gtccaacagc cgatcttttc
     6721 ctgacacgat ctcatagccc atatggccgg gggtgtgacc ctggatggcg acggcagtaa
     6781 tgccgggaac aacgatggcg cccggctcga acgtcttgac gtggccggtc accgctttga
     6841 caacgtcggc cgcgtcttgg cggctctgca tgaaggccca ctcggcggcg gacatgtgca
     6901 cggtcgcatg cgggaaggcc ggctggccgt cggccgcgac caggccgccg atatgatcgc
     6961 catggctgtg tgtgatgagc acatcggtta catcaccggg ggcgagtccc gccttcccaa
     7021 ggctttgcag cagaacaccc ttgaccttcg gcccaagccc cgtatcgagc agaacgacat
     7081 gcttgccgtc tttcaccaac aacgcggtga cgctcaagct gatgctatcg gcaggtgcac
     7141 cggccgcttg caacaccttg cccacctcgg cgggacccac agcggcgccg aataccgagc
     7201 cgtcgttcgc gcccacgaac ttcgcatcgg ctagggcgaa agcctgcaag gcgcctatct
     7261 ggaacggcgt tgccgcgggt tcaacgggaa cgggacctgc ggcggcggcc aatcgcgccg
     7321 cgccgagcgc cgttgcaatc aagagaatcg aggtcaatcg cttcatcggg tcactccgca
     7381 ggtattttcg cgcatggtag cgtattacgc gtgggtctcg cgaacgcggt tcggctcaag
     7441 gctcgtagat cgcgcccacg aacaacagcg tgccgctgcg cccatcttcg atggcacaga
     7501 agaacggccg atcgacgatc atccggaatg gctcggccgg ccgcgcggca agcgtggctc
     7561 gcatggtggt cgtcgtaacg gccgccgctt cggagccctc ctcgtccact ttccagtaca
     7621 ccgactgcag cacggccgag atgaagaagt tgcggtcgtg cgcggcgccc ggcacgggcg
     7681 agaacatcga cgtcagctgc gcggcgtccg cgccgccggc gtgaaacgcg cgattcatac
     7741 ccaacgcctg cagcgctgcg ttcaggcgat acttggactt gagctcgaag cgcggcagtt
     7801 cgatgtttcc cggacggcgc acgaagcgcg ctttccatgc ctgccagttc tgcggtgtca
     7861 actgcgcttc caatgccgcc aaactcgacg tactcgaggg caggagcacc tccaaaccca
     7921 aatctcccgc accgaacggc aggcggatcg cctgcatcgc cggcgtctcg aagtattcga
     7981 agcgtccgga ctgtgccatg cgcatcagct gtttgaccga cccgccggcc aagttgaagt
     8041 cgcgcagttt cgtttgcgcg gcgtcgaatt tgaaggtcca ctcgcctttg aagtacacgg
     8101 cgttcagcaa cagcgcgaga tcggcgcgat cgattcggtc gatgacttgc gtgatcttgc
     8161 cgtgcgtgtg ctcggacgcc cagtcattga tgcgcgccgc cgcgccggga cttgaaaaat
     8221 caagatccgt catctcggcg tcgtacgagg cttgcgcttg cttcaagtac tcgggcttga
     8281 gggtcgcgcg ccgattgtcg acccacagcg aatccgccac ggcgagagtg acgctcttcg
     8341 ccggcgtgcg gatggtcttg atgagcgctg cattggcggc gttgatggca tcgagttccg
     8401 atccgtcaag cgacagcgcg ctgcgcatct cgtcgcgcgt ttgaccgtcc gcgccgttca
     8461 gcaccatggc aagatcgagc gccgcgctca ccggagagag gatgacattg cccgtagggg
     8521 tcgccttttg aaccgcgttg agcaaccgaa agccgaatgc attgtccgcg gcggccacgt
     8581 ccacggcagc gggcgccgcc tgcgcagctg aggccgcgaa cgcggccgcg gccgcgatac
     8641 ttgcaagcca tgacgggtgc cgattcatct gcgcgccgct tgcgagctcg cggcgccgcc
     8701 cgcgagttga ttgatcaagc cgacgccggt ggggctcccc caaccggtca ccagatcgta
     8761 ccccggtacg gcgttgaaac cactgccgct cccggtcgtg ggcgggttac tgccgctcgt
     8821 gatgtcgtgc agcgcgcccg agtagccgct actgacgccc aagttgtaga gcgcggggtt
     8881 caagaaaccc accgtgcctt tgccattcgc caccgactgc tgattcacca gcgccaggaa
     8941 cccggcccag cgaggcgtcg cgaaactcgt gccgccgtag ccggtcatga aagcgccgtt
     9001 gctcaccgtc ggattgtcga aatccgcctc tgccgctaca tcgggcgagt tgcgcaaggt
     9061 ggtcgagcct tgattggagg cattgatgac acccgccagt tgctgccagc tcgggatcgg
     9121 cgtccctgag atgaagccgc cgccgctttg cggccacgcg gtttccgccg accaggcgcc
     9181 gcccggtccc gccgtcgtca acaccgtgcc gccgacttgc gtgatgtact gatccacggc
     9241 cggaaaatcg taggtcgaac tattcaactc accgtcatcg ccgctggcgg tgacgaacgt
     9301 ctggccctgc gccgcaaact gctggaatat cggatcgtcg ctcgcggcat ccgccggatt
     9361 ccagccccac gaggagctca agaccttggc gatattgtcg gtcgccatct gattcaggat
     9421 ctgggtgttg ctggttccct catagaagag aatctgtttg atgccggggg ccatgccgat
     9481 ggcgttcacg atatcgagca cctgttcgcc gtcatcgcag gtggaactcg gtgcagagct
     9541 gaccgaggtg caggcgccgt tgaagccgcc gaccaataca ttattgatgg gtacgctcga
     9601 ggtcatgccg gtcttcgaat aatagagctg cacatcgctc gcgagatagc cgtcgaatga
     9661 aaagatgccg atggactgtc cggcgccggt gagcggaccg ttgccgtaat acgccgcgcg
     9721 catgtcgctc ggcaaaaact ctcccgacgt cccggagccg ctggcaagat gcgccgcaac
     9781 gccatggcgg aacatcgggt gcggcagcac ggagttgttg aggccggtga tctgcagcag
     9841 cggcacagtg aggccgacgg tgctcggctc gcgattcggt gaatagaaag tgcgcccctc
     9901 ggtcggatgc cgatagttgt ttatcgtgac gtggaaggca cggttgaccg tgttcaccga
     9961 cccttcgacg gcgaccagcc ggcggttggg cgtggtggca atgactttga ggccgtttct
    10021 cttcgcccag gcaactactg cggcgtagtc gcgcgccgtc ggcgcgaagc gcgcggtgaa
    10081 atccgacacg ctcagatact gatgaaactt gcggctcttc gggtcgtaca gatcgtgcaa
    10141 caactgcgtc agctccgcct gattgcgcag cggcagttgg ataaccggcc gcaggatctg
    10201 ttgcgcgccc accggtcccg tcatggccgc cgtaccgtcg gcgaccgacc gcggaagatg
    10261 cggcgccagc atcggctggg cgccgggtac ggcggtggcg cccgaccccg cgggagcggg
    10321 cggcgagctg ctgcaggaga ttgtcccgaa gatcatggcc agggtgacgg ccgaggcggc
    10381 taaattgaag ggcttcatca gcttgctcca gagtcgttga acacgggtct catccagagt
    10441 ccatacagca aaccgtagcc aatcttacgc gcagcgagcc gcggcgcgct gcaattctta
    10501 caggggcacc gccgcgactc ctcgaaccga ttgtcacgag ccgcctcata gttgcactta
    10561 cgcttttgtt tggtgcgctg atgcgacgcc gacccagcgt cgcctgatcg caattcgact
    10621 tgaggacgcg aatgatttcc cccggcagaa aaagtcgaca gcgggcccca cactttgcag
    10681 tgcgaggccc gccgcgggtc gattctcata tagtagacag aaaccaacga tcgttcgttt
    10741 tcgcttacga cctcactccg ctacaccggt aatgatggct cccatcttcg gcgtaccaat
    10801 accggtcgcg agatcgtagc cgcgagtggc cgggaagtag ccgttgttag tcgggaatat
    10861 cgtgtcgctg ttcgacggcc cgcgtcccgt gatgtcgtgg aagaagccct gataatcgac
    10921 gttatacaga cggtaaagca gcgggttcgc gttcccgatc cggtcatgcc agaagccgtc
    10981 gcgatcagca atgattgccg accagaaagg cgatgacagg ctggtgccgc
//
LOCUS       DASWZT010000186         3677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_40232, whole genome shotgun sequence.
ACCESSION   DASWZT010000186 DASWZT010000000
VERSION     DASWZT010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40232"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VGE92_04965"
     CDS             complement(<1..185)
                     /locus_tag="VGE92_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313202.1"
                     /translation="MSHPGIPVIALLLMGAIGAATPAPGQSVHPELWAAAPAQAADPA
                     TEAFVEQLLSQMSVEEKV"
     gene            388..1986
                     /locus_tag="VGE92_04970"
     CDS             388..1986
                     /locus_tag="VGE92_04970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HEY1313203.1"
                     /translation="MPSSTPAFGVLSLLDWVVVATYAGGLLLLGVFVSRRRIAPVDYF
                     LASRASRWPVIGLALLASNMSSTALVGLAGGAYAMGISVYDYEWSAVVILVFFCLYLL
                     PSIIRSQTYTMPEFLEKRYDGRVRLHFALLTLFLTIFVDSAGVLYSGSLVCQLLFPAW
                     PLWLIVTLLAGTAGLYTTLGGLKAVIYTEAVQAIVLMSGALIISIGAFSRAGGWHAVM
                     QGVNPAAISLIRPAGDAGVPWPGLLLGIPLLGFYYWCTNQSIVQRMLSAKNIDHARWG
                     ALFAGLLKLPVLFLIVLPGTCALLLFPKLARPDLVYPHLILKLLPTGLVGLVVAGFVA
                     ATMVAIASMLNSASTLITMDVIKQVRPTLSDRQVVRIGRICTVALLVVAVAWAPQLQL
                     FPSLWQYLQAVLAYAVPPVVALFLVGMFWRGANADGAAAAMIVGSLCGFSLFLINAVL
                     HWTQFHFLYAAPILTLLDAAILVGVSLRSGAGLSAASAATPWKLDFSRAEQLRQQLVP
                     LWQDYRFQAAALLALTAAVVIAFR"
     gene            2002..3078
                     /locus_tag="VGE92_04975"
     CDS             2002..3078
                     /locus_tag="VGE92_04975"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011783578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEY1313204.1"
                     /translation="MGTDRRILELFDIEIPIIQAPMAGPGTPELAVAVSSAGGLGSLP
                     CAQLDLQQARASLELIRRETSRPINLNFFCHTPPQANAERTLNWLARLAPYYAEFGIE
                     PDAAAASSGRRAFDADFCTLLEAYRPKVVSFHFGLPQTELLSRVKAAGCKIISSATTV
                     AEARWLEQHGCDAIIAMGSEAGGHRGSFLAEDMSRQIGTFALLPQVADAVAVPVIAAG
                     GIADARGIVAAFALGAAAVQIGTAYLFCPEAKVSKIYLDALLRAADEDTMITNVFTGR
                     PARGIVNRLMREQGPLSAFTPQFPLAAAAVAPLKARAEAAGSGNFTNLWSGQAVHLAA
                     RSRPAGELTRRLWDEASGKLGARG"
     gene            complement(3088..>3677)
                     /locus_tag="VGE92_04980"
     CDS             complement(3088..>3677)
                     /locus_tag="VGE92_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313205.1"
                     /translation="GSVGAAAAPVSRLEVRIVTGNEELGAGSSLELRIYETGRNVRRV
                     PLVHGEEWPPDSTHIIPVTLSEVLEPRNVERFSLYYRSASPLAPGLEIIAADVELPST
                     KGSPDLLLNATLSGVIARQGELATSDLDQASLVCRNDADCDDHRTCNGRERCAPHAAG
                     ADARGCVKGTPVVCPVNQVCGEGIGCRGIGESPKP"
BASE COUNT          596 a         1170 c         1148 g          763 t
ORIGIN      
        1 accttctctt ccacactcat ctgggagagc aattgttcga cgaatgcctc ggtggccgga
       61 tccgccgctt gagcgggcgc ggcggcccaa agctcaggat gtacactctg gccgggggcg
      121 ggcgtcgctg cgccgatcgc acccatcaac aatagagcga taaccgggat gcctgggtgg
      181 gacacccaat tagacctaca gcatgcggat cgcgtacttg atttcgccat gaatgcgcta
      241 taactatctg taatatttat atttctagtc gttacgggaa agccaacgat cccgccaaaa
      301 atgccgtcgg aagcaaagct tacatgaata acgggaaaaa tgtcagcgct gtcaatgaag
      361 cgccaagacc gggcccaaag gccggcgatg cctagcagca cccccgcctt cggtgtgctc
      421 agcctgctcg actgggtggt cgtcgcgacc tacgctggag gtctgctgct cctgggcgtt
      481 ttcgtctcac ggcgccggat cgcgccggtc gattactttc tggcctcgcg cgccagtcgt
      541 tggccggtca ttggcttggc gctgttggcc tccaatatgt cgtcgacggc tctggtcggg
      601 ctcgccggag gcgcctatgc aatgggaatc tcggtctacg actacgaatg gtcggcggtc
      661 gtgatactgg tgttcttctg cctgtacctg ctgccctcca tcattcgcag ccagacctac
      721 accatgccgg agttcctcga gaagcgttac gacggtcgcg tgcggctgca cttcgcattg
      781 ctcacgctat ttctcaccat cttcgtcgat tctgccggcg tgctgtacag cggctcgctg
      841 gtctgtcaat tgctgttccc ggcatggccc ttgtggctga tcgtgacctt gttggcaggt
      901 acggcgggac tctacaccac gctcggaggc ctcaaggcgg tgatctacac cgaggcagtt
      961 caagccatcg tactcatgtc gggcgctctc atcatatcga tcggcgcttt cagccgcgcc
     1021 ggtggatggc acgccgtcat gcaaggcgtg aatcccgcgg cgatttcact gatccgaccg
     1081 gccggtgacg cgggcgtgcc gtggcccggg ttgttgctcg ggatcccctt gctgggtttc
     1141 tactattggt gcaccaatca atcgatcgtg cagcgcatgt tatccgcgaa aaacatcgat
     1201 cacgcgcgct ggggagcact gttcgcgggt ctgctcaagc tgccggtgtt gtttttgatc
     1261 gtgctcccag gaacctgtgc attgttgctg ttccccaagt tggcgcggcc cgacctcgtg
     1321 tacccgcacc tgattttgaa actgctgccg accggtttgg tcggcctggt cgtcgcggga
     1381 ttcgtcgcgg cgacgatggt cgccatcgct tcgatgctca actctgcatc gacgttgatc
     1441 accatggatg tgatcaagca ggtccggccg acgttatccg accggcaggt ggttcgaatc
     1501 ggccgcatct gcaccgtcgc tctcttggta gtggcggtcg cctgggcacc gcagctgcag
     1561 ttgttcccgt cgctctggca gtatttgcag gcggtattgg cctatgcggt accgcccgtg
     1621 gtcgcgctgt ttctggtcgg tatgttttgg cgcggagcca acgccgacgg cgcggccgcc
     1681 gccatgatcg tgggttcgct ctgcggtttt tcgctgttct tgatcaacgc ggtgcttcat
     1741 tggacgcagt tccattttct ctacgcggcg cccattctca cgctgctcga tgcggcgatc
     1801 ttggtcggcg tgagtttgcg cagtggcgcc ggcctctctg cggcgagcgc ggcgacgcca
     1861 tggaagctcg acttcagccg tgccgagcag ctgcgccaac agctcgttcc gctgtggcag
     1921 gactatcgct tccaggccgc cgcgttgctc gcgctcacag ccgctgttgt aatcgcgttt
     1981 cggtagtcaa gcggggaagc catgggaact gatcgacgca ttctcgagct ttttgatatc
     2041 gagatcccca tcatccaggc gcccatggca ggacccggga cgcccgagct cgcggtcgcc
     2101 gtcagcagcg ccgggggctt gggatccctg ccgtgcgcgc aactcgattt gcaacaggcg
     2161 cgtgcgtcgc tcgaattgat ccgccgcgaa acgtcgcggc ccatcaatct taattttttc
     2221 tgtcacacgc cgccgcaggc caatgccgaa cgcacactga actggctcgc gcgcctcgcg
     2281 ccgtattacg cggagttcgg catcgagccc gacgcggcag ccgcgagtag cggccgccgc
     2341 gcattcgatg ccgatttttg caccttgctc gaagcctatc gtcctaaagt cgtgagcttt
     2401 catttcggct tgccgcaaac cgaactcctg agtcgggtca aagctgccgg ctgcaaaatc
     2461 atctcctccg cgaccacggt ggccgaggca cgctggctcg aacagcacgg ctgcgatgcg
     2521 attatcgcca tgggatccga ggccggcggt catcgcggca gctttctggc tgaggacatg
     2581 tcgcggcaga tcggcacttt cgccttgctg ccgcaggtgg ccgacgccgt ggcggtgccg
     2641 gtcatagcgg ccggcggcat cgccgatgcg cgcggcatcg tcgcggcgtt tgcactcggc
     2701 gcggccgcag tgcagatcgg cactgcgtac ctgttttgtc ccgaggcgaa ggtgtcgaag
     2761 atctatcttg acgcgctgct gcgcgcagcg gacgaggata cgatgatcac caatgtattc
     2821 acgggccggc ccgcgcgcgg catcgtcaac cgcctgatgc gcgagcaggg accgctgtcc
     2881 gcgttcacgc cgcagtttcc gctcgcggcg gcggcggtgg ccccgctcaa ggcaagagca
     2941 gaggctgccg gcagcggcaa tttcaccaat ctgtggtccg ggcaggcggt gcatctggca
     3001 gcgcggtcgc ggccggctgg ggaactcacg cgtcgactct gggacgaagc gtcgggcaaa
     3061 ctcggcgccc gcggttgaac tgccgcgtca gggtttcggg ctctcgccaa tgccgcggca
     3121 gcctatcccc tcgccgcaaa cttgattcac ggggcacacc acgggtgtcc ctttcacgca
     3181 gccgcgcgca tcggcgcccg ccgcatgcgg cgcgcagcgc tcgcgcccgt tgcaggtgcg
     3241 gtgatcatcg caatccgcgt cgttgcggca aacgagcgac gcctgatcga gatcagaggt
     3301 cgcaagctcg ccttgccggg cgataactcc ggagagcgtc gcattgagca gcagatcggg
     3361 cgagcccttg gtcgaaggca gctcgacatc ggcggcgatg atttccagcc ccggcgcaag
     3421 cgggctcgcg ctgcgatagt acaaactgaa gcgctcgaca ttgcgcggct cgagtacctc
     3481 gctcaatgtc accggtatga tatgagtcga atcgggcggc cattcctcgc catgcaccag
     3541 aggcactcgc cgcacgttac ggccggtctc gtaaatgcgc aattccaacg agctgccggc
     3601 gccgagttcc tcgttgcccg tgacgatgcg aacctcgaga cgagacacgg gcgccgccgc
     3661 cgcgccgacg ctcccga
//
LOCUS       DASWZT010000187         4586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_49671, whole genome shotgun sequence.
ACCESSION   DASWZT010000187 DASWZT010000000
VERSION     DASWZT010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49671"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2252)
                     /gene="putA"
                     /locus_tag="VGE92_04985"
     CDS             complement(<1..2252)
                     /gene="putA"
                     /locus_tag="VGE92_04985"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF008869.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEY1313206.1"
                     /translation="MAANVNPSAPRHSLETLRQAMREHGEWTEERAVTGLLHALELTG
                     GARHRAVATAVALVDGARARRDERPFLDAFLQEFGLSNQEGIALMCIAEALLRIPDDA
                     TADRLIAEKLATGDWSSHSGKSESLFVNASTWGLMLTGGILELDPSIQADTTGWMRKL
                     TRKAGEPMVRLAVRRAMRIIGGEFVVGRSIEEALARSAREADVGLCSFDMLGEGARTL
                     KDAQRYLESYEHAIDVIGGAAKGRAPHEASSISIKLSALEPRYALLQHERVMQRLVPL
                     LIGLVRRAAGLGIQLTIDAEEADRLDLSLDVIEALAQDSATRDWPGLGLAVQAYGKRA
                     LDVIEWVAATARANGRRMTLRLVKGAYWDSEIKRAQERGLGGYPVYTRKVTTDVSYLA
                     CAGRLFAHADVIYPQFATHNAHSIAAVLQLAPLGTDYEFQRLHGMGRLLYAEAARQIE
                     DFPRVRIYAPVGEHKDLLAYLVRRLLENGANTSFVNRFMDEQVPVTEIVRDPISELER
                     LESHAHPRLPLPTVLYSDRRNSRGVDLGNPQQVARLRQELATRRAAKPEAGPIVNGVL
                     LPGATHAVTNPADRRDTVGSTRDATPAEIVAAFDAGAAAQAAWNLSGAEARAERLERA
                     ADLLEANRADFHELLVREAGKTIADAISEVREAADFCRYYALKARQQFSPRRLEGPTG
                     ELNELSLHGRGVFACISPWNFPLAIFAGQVTAALAAGNTVVAKPAEPTPLIAARFVKL
                     LHEAGVPPQAV"
     gene            complement(2279..3232)
                     /gene="rocF"
                     /locus_tag="VGE92_04990"
     CDS             complement(2279..3232)
                     /gene="rocF"
                     /locus_tag="VGE92_04990"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HEY1313207.1"
                     /translation="MSAARTASLIGAPTDVGASDRGASMGPEALRVAGIHAALTQRGL
                     DVVDCGNVSGPPNPGLIKTNGYRNLAQVTAWNHEVHDAVFSELREQRLPILLGGDHSL
                     SIGSVSAVARHCRERRKSLRVLWLDAHTDFNTRELSPSGSLHGMPVSCLCGFGPRELT
                     QIGGLSPAISARWVRQIGIRSVDSGERRFVHEQNLDVSDMRFIDEMGMRKVMELALRD
                     MDDNTHLHVSFDVDFLDPEIAPGVGTTVSGGLSYREAQLCMEMICDTGLIASLDIMEL
                     NPALDVRNKTALLAVDLVESLFGKSTLMRKPLAFPSALIDT"
     gene            complement(3229..4293)
                     /locus_tag="VGE92_04995"
     CDS             complement(3229..4293)
                     /locus_tag="VGE92_04995"
                     /inference="COORDINATES: protein motif:HMM:NF005762.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HEY1313208.1"
                     /translation="MVDYIGTARVAQLVSRIGPARFMAELAAEIEADYRRWPQFEKSA
                     RLASHSANGVIELMPTSDGKLYSFKYVNGHPQNTAKGLLTVTAFGVLADVATGYPLLL
                     SEMTFVTALRTAAISALAACKMARPASRVMAMIGNGAQSEFQAIAFHQMLGVRELKLY
                     DTDPRATAKLVRNLTRLQLPDLTLTRCGGVAEAVRGADIVTTVTADKCNATILTPQMI
                     EPGMHLNAVGGDCPGKTELHVDILRANGVRIVVEFEPQARIEGEIQQLGGAGRVIELA
                     DVLLGKQPARLTDGDVTLFDSVGFALNDFSALRYLHRLNAQLPDGGQRIDLIPEPDDP
                     KDLYAGLGRERAPLERRRFA"
BASE COUNT          827 a         1532 c         1435 g          792 t
ORIGIN      
        1 acggcctgcg gcggcacgcc cgcctcgtgc aatagcttca cgaagcgcgc ggcaatcagc
       61 ggcgtcggct ccgccggctt ggccaccacg gtattgccgg ccgccaaagc ggccgttacc
      121 tggccggcaa aaatcgccaa cggaaaattc cacgggctga tgcaggcgaa cacgccgcgt
      181 ccatgcagag acagttcgtt caattcgccg gtcggtccct cgagcctgcg gggcgagaat
      241 tgctgccgcg ccttgagcgc gtagtagcgg cagaagtccg ccgcctcgcg cacttcggaa
      301 atggcgtcgg caatcgtctt gcccgcctcg cgcaccaaga gctcatgaaa atccgcgcga
      361 ttcgcctcca acaagtccgc cgcccgctcg agtcgctcgg cgcgcgcctc ggcccccgac
      421 aggttccagg cggcttgcgc cgccgcaccg gcatcgaagg ccgcgacgat ctcggccggg
      481 gtcgcatcgc gcgtgctgcc caccgtatcg cgccgatcgg cggggttcgt caccgcatgc
      541 gtggcgccgg gcagcaagac gccgttcacg atgggcccag cctcgggctt ggcggcgcgg
      601 cgagtcgcca gttcctggcg caagcgcgcg acctgttgcg gattgccaag gtccacgccc
      661 cgcgaattgc gacgatcgct atacagcacc gtcggcagcg gcagccgcgg atgcgcatgg
      721 gattccaggc gctcgagttc cgagatcgga tcgcgcacga tctcggtgac cggcacttgt
      781 tcatccataa accgattcac gaacgaggtg ttggcgccgt tctcgagcag ccgccgcaca
      841 agatacgcca gcaagtcctt atgctctccg accggcgcgt aaatgcgtac gcgggggaaa
      901 tcctctattt gccgcgccgc ttcggcgtac agcagccggc ccatgccatg cagccgctga
      961 aattcgtagt cggtgccgag cggcgccaac tgcagcaccg cggcgatgct gtgcgcattg
     1021 tgagtggcga actgcgggta gatcacatcc gcgtgagcga acagtcgccc cgcgcatgcc
     1081 agatacgata cgtccgtggt gaccttgcgg gtgtagaccg gatatccgcc gagaccgcgc
     1141 tcctgggcgc gcttgatttc cgaatcccaa tacgcaccct tcaccaaacg cagcgtcatg
     1201 cgccggccgt tggcgcgtgc cgtggcggcc acccattcga tgacgtccaa ggcacgcttc
     1261 ccgtaggctt gtaccgccaa tcccaagccg ggccaatcgc gcgtcgccga atcctgggcg
     1321 agcgcttcga tcacatccaa tgacaaatcc agccggtcag cctcctcggc gtcgatggtg
     1381 agctgaatcc cgagaccggc ggcgcggcgc accagcccta tcagcaacgg caccaggcgc
     1441 tgcatcaccc gctcgtgctg cagcaacgcg tagcgcggct cgagcgcaga gagtttgatg
     1501 gaaatactcg aggcctcgtg cggcgcccgc cctttggccg cgccgccgat cacgtcgatg
     1561 gcgtgctcgt aggactcaag atagcgctgc gcgtccttga gagttctcgc gccctcgccc
     1621 agcatgtcga aggaacacag ccccacgtcc gcctcgcgcg cgctgcgcgc cagcgcctct
     1681 tcgatgctgc gcccgaccac gaattctccg ccaatgatgc gcattgcgcg gcgcacggcg
     1741 agacgcacca tcggctcgcc ggccttgcga gtcagcttgc gcatccagcc ggtcgtgtcc
     1801 gcctggatgg agggatccaa ttcgagaatg ccgccggtga gcatcaggcc ccaggtcgac
     1861 gcattgacga acagcgactc cgattttccg gagtgcgacg accagtcgcc ggtggcgagc
     1921 ttctcggcga tcagccgatc ggcggtcgcg tcatccggaa tgcgtagcag cgcctcggcg
     1981 atgcacatga gcgcaatgcc ttcttgattc gacagtccga actcttgcag gaaggcgtcg
     2041 aggaatggcc gctcgtcgcg ccgcgcgcgc gcgccgtcga ccagcgccac cgcagtggcc
     2101 accgcccgat gccgcgcgcc gccggtgagt tcgagcgcat gcagcaggcc cgtcactgcc
     2161 cgctcttcgg tccactcgcc atgctcgcgc atcgcctggc gcagggtctc caaggagtgg
     2221 cgcggcgcgg atgggtttac gttggcagcc atgactgatc cttgaggcga atcccgcttc
     2281 aagtatcgat aagagcgctc gggaacgcca gcggtttacg catcaaagtg ctcttgccga
     2341 acagcgattc gaccagatcg acggcgagca gcgcggtctt gttgcgcacg tccaaggccg
     2401 ggttcagttc catgatatcc aaggaagcaa tcagacccgt atcgcagatc atttccatgc
     2461 acagctgggc ctcgcgatac gacagcccgc ccgacacggt ggtccccacc ccgggagcga
     2521 tttccggatc gaggaagtcg acatcgaagc tcacgtgcag gtgggtattg tcgtccatgt
     2581 cgcgcaacgc cagctccatg accttgcgca tgcccatttc gtcgatgaaa cgcatgtcgg
     2641 agacatcgag attttgttcg tgcacgaagc gccgctcgcc gctgtcgacg ctgcgaatcc
     2701 cgatctgccg cacccaacgt gcactgatgg ccggcgacag gccgccgatc tgcgtcagct
     2761 cgcgcggtcc gaaaccgcac agacacgaca ccggcatgcc gtgcaggctg ccgctcgggc
     2821 tcaattcgcg cgtattgaag tcggtatgcg catcgagcca cagcacgcgc aaggacttgc
     2881 gccgctcgcg gcagtggcgc gcgacggccg agaccgaccc gatgctgagc gaatgatcgc
     2941 cgccgagcag gatcggcagc cgctgctcgc gcagctcgct aaacaccgca tcgtgcactt
     3001 catgattcca agccgtcacc tgcgcgagat tccgatagcc gttggttttg atcagcccgg
     3061 gattcggcgg tccgctgacg ttgccgcaat ccaccacatc gagaccccgt tgggtcagcg
     3121 cggcgtggat gccggcgaca cgcagcgcct cgggacccat ggaggccccg cgatcgctgg
     3181 caccgacatc ggtgggtgcg ccgatcaggc tcgcggttcg cgctgcgctc atgcgaacct
     3241 ccggcgctcg aggggcgcgc gttcacggcc gagaccggca tagagatcct tcggatcgtc
     3301 cggctcggga atgagatcga tgcgctggcc gccgtccggc agctgcgcat tcaaacgatg
     3361 caagtagcgc aacgcggaga agtcattgag cgcgaaaccc accgaatcga acagcgtgac
     3421 atcgccatcg gtcagacgcg ccggctgttt gccgagcaat acgtccgcca gttcgatcac
     3481 ccggcccgcg ccgccgagct gctgaatctc gccttcgatc cgcgcttgcg gctcgaattc
     3541 gaccacgatg cgcacgccgt tggcgcgcag gatgtccacg tgcagctcgg ttttgcccgg
     3601 gcaatcgccg ccgactgcat tcaaatgcat gcccggttcg atcatctgcg gcgtgagaat
     3661 ggtggcgttg cacttgtcgg cggtcaccgt cgtcacgatg tccgcgccgc gcacggcctc
     3721 ggcaacgccg ccgcaacgcg tgagggtgag atccgggagc tgcagccggg taagattgcg
     3781 aaccaatttt gcggtggcgc ggggatccgt gtcgtagagc ttgagctcgc gaacacccag
     3841 catctggtga aacgcaatcg cctggaattc gctttgcgcg ccattgccga tcatggccat
     3901 cacgcggctc gccgggcggg ccattttgca ggcggcgagc gccgatatcg ccgcggtgcg
     3961 cagcgccgtg acgaaggtca tctccgagag cagcaacgga tagcccgtgg cgacgtctgc
     4021 gagcacgccg aacgccgtga ccgtgagcaa tcccttggcc gtgttctgcg gatggccgtt
     4081 gacgtacttg aacgaataca gcttgccgtc gctggtcggc atgagttcga tgacgccgtt
     4141 cgcggaatgg ctggcgagcc gagccgattt ctcgaactgc ggccaacgcc ggtaatccgc
     4201 ctcgatctcg gcggcgagtt ccgccatgaa gcgagccggg cctatacggg ataccaattg
     4261 ggcaacccgc gcagtgccga tgtagtccac catgttgttc ccccgatgca gccaaacgct
     4321 agtgtcaggg gagtctcggc taaataatag ccagaaagtt gaccaataag tgtcataatt
     4381 tgtgcatatc gtcagataat attgacgttc tgttcaattg gagagtaacg ccatggacac
     4441 caccgatcgg gagctactgt cgttattgcg cgataacgcg cgcctctcgg tggcctccct
     4501 cgccaaggcg ttgcgggtcg ctcgcggcac cgtgcagaac cggctggcgc gcatggaaaa
     4561 ggacggcacc attaccggct ataccg
//
LOCUS       DASWZT010000188         5946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_30534, whole genome shotgun sequence.
ACCESSION   DASWZT010000188 DASWZT010000000
VERSION     DASWZT010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5946
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30534"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VGE92_05000"
     CDS             complement(<1..925)
                     /locus_tag="VGE92_05000"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1313209.1"
                     /translation="MGVAGPRVIAEFGLSAAQAGVIFSAATIGLFLGAATGGRLADLW
                     GRKRTLGMSLLIFGICSLATALSDGPKALFAARLITGIGLGGAMPNFISLASESAHPQ
                     RRISAVTLVMAGMPFGGAIAACIALGAHWGWSWHSIFYAGGVAPILLAVLLFYVLPDA
                     SHPRGQARAAALRLDKVTTVLFGGDRARTTLLLWIAFFFTQLILLLMLNWLPSLVKNL
                     GFTRDQASMTSICFNLAGSVGAGLLGLLHAGEKRRRWVAVTYAGMALALAGLASVGSN
                     FVLAALACALAGIFIVGAQLVLFALAPLYYPT"
     gene            complement(1068..1529)
                     /gene="soxR"
                     /locus_tag="VGE92_05005"
     CDS             complement(1068..1529)
                     /gene="soxR"
                     /locus_tag="VGE92_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HEY1313210.1"
                     /translation="MRGKDPATVIRKELGVGEIAARSGIAVSALHFYETKGLIKSRRN
                     PGNQRRYPRETLRLVAVIKVAQRIGMPLATIGRALATLPNGRAPTAADWRRLSAVWKR
                     DLEERIDRLTRLRDSLSGCIGCGCLSMKACPLRNPGDKLREEGCGPRLLDP"
     gene            1581..2990
                     /locus_tag="VGE92_05010"
     CDS             1581..2990
                     /locus_tag="VGE92_05010"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1313211.1"
                     /translation="MLFNNGAIRGATWRDLYAAGLLNRFAVLCAGIWLHAVDTLVTAT
                     ISPAIVEDIGGVAFINWTFLLYEVGAIVAGAATATLCRRIAIKRVLAFAALCYGGGCL
                     IAGFAPSIGVLLAGRLVQGIGGGMLLSLCYLAIQAWFEPCWWSRLFGIVAVIWGAGSL
                     LGPLIGGIFAGLHSWRMTFFVFGIQAGVLWILARLWLPAETPARREREVFPLKPLLTL
                     MVAILLIAESALAPTIQISIIGVVLGLGVLYCAARLDARLNSKLFPVQILDFRHPVGA
                     GLLMVFALSAATTGFWTYGPLILKILFDTHPLVCGYILAGEAVAWSLATMAVSKVPID
                     CDRWVIRVGASLVALGAGGFALAVPAGMLAGLILCALLQGVGFGLCWPSIVHRLARFS
                     AADERALAVTSPETVQRIGYAVGTAAVGIAANLSGLAAGLSIAAVKAAAFWVFAAFLP
                     VLLLALMSAWRFTRDAAEA"
     gene            3022..3693
                     /locus_tag="VGE92_05015"
     CDS             3022..3693
                     /locus_tag="VGE92_05015"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEY1313212.1"
                     /translation="MTTFLLAFGALFSIVNPLSGAFIFFGATRELDPRVRSQVSRWVA
                     IYSFSIVAASLYVGAYVLSFFGISIPVLRVAGGIVVALSGWRMLNEPDYTQQRRSETP
                     SPRSMDVPPSRLAFYPLTMPLTTGPGTISVAISLGAGRPRGFHAPLLEFFVETLAAVA
                     LLALLVYAFYRNSARLAGLMGATGTTIVVRLSAFLLFCIGIQVLWNGASELIGSLPLG
                     TAGTS"
     gene            3706..5862
                     /locus_tag="VGE92_05020"
     CDS             3706..5862
                     /locus_tag="VGE92_05020"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY1313213.1"
                     /translation="MSIGFDRRRFLGTAGALSLTLLSKQLRAYATPAAPVAPVARIDV
                     VRDTYFGETLSDPYRWMENANDPDWLPFLRGQNAHTHAILDALPMRASLLRRIQELSS
                     NTVATGRVQRAGGLTFFIQRPQGADNFKLFVRKGGRDRVLIDPTKAGDTTGHTSLDWW
                     RAAPDGRHVVYGLSKNGSEDSTLRVLAVADGRDLAERIGNAQQANPAWLDDGSGFFYN
                     QLTAAVATPERFLDSQARLHRLGTDPNSDPVLMKRGLVPGVEYERIQAPYIVTYPGAR
                     HAILLLSDVRPEGRAFIAPLADVLAGRPRWTPFAAFEDEVTDFELEGEDLYLLVNHGT
                     ARGRIVKTSAASPNIATAAVVVPQGPYVIQDISRARDGLYLRLMDGGLSRLQRLEHGG
                     RITDIALPFEGSLGSVYTAADEDGALLSLTGWLEPAAIYSVSTEGRVADTGITPKPPI
                     DVAPYVARRFFATAKDGTKIPYSVVHRKDLTLDGSAPAWITAYGSYGLSPNTPAFVGR
                     ALALIDAGAVVGYANVRGGGEYGREWHKAGQLANKPNTWRDLIAVCEDLVAKKYTSPS
                     HLAIGGRSAGGITVGRAMTERPELFAAVVDGVGWSNPLRYVVEQDSYTEEPEWGAISN
                     AEGYRFLKLIDSYQSVKDGTRYPAVLLTTGVTDPRVAPFHVAKMTARLQAASSSGKPI
                     LLRVDFDAGHGIGSTRSQQDREVADTHAFLLWQLRG"
BASE COUNT          968 a         1963 c         1873 g         1142 t
ORIGIN      
        1 gcgtggggta atacaacggc gcgagggcga acaaaacgag ttgcgcaccg acgatgaaaa
       61 tccccgcgag cgcgcacgcg agtgccgcca gtacaaaatt gctgccgacc gaggcgaggc
      121 ccgccaatgc gagcgccatt cccgcatagg tgactgcgac ccagcggcgg cgcttttcac
      181 ccgcgtgcaa cagtccgaga agccctgcgc ccacgctacc ggcaagattg aagcaaatgg
      241 aggtcatgct cgcttggtcg cgcgtgaagc cgagattctt caccagactc ggcaaccaat
      301 tgagcatcag cagcaggatc aactgcgtaa aaaagaaggc gatccacaac aacagggtgg
      361 tccgcgcccg gtccccgcca aagagcaccg tggtcacttt gtcgagccgc agcgcggcgg
      421 cccgagcctg cccgcgcggg tgcgatgcat cggggagcac ataaaacagc agcaccgcga
      481 gcaaaatcgg tgccacgccg ccggcataga atatcgaatg ccaactccaa ccccagtggg
      541 cgccgagcgc gatgcatgcc gcgatggctc cgccgaacgg cattcccgcc atgaccaggg
      601 tcaccgcact gatgcgccgc tgcggatgcg cggattcaga ggcgagggag atgaagttcg
      661 gcatcgcacc gcccaatccg attcccgtga tcagccgagc ggcgaacaag gccttgggcc
      721 cgtccgacaa cgcggtcgcc aaggaacaaa tcccgaaaat cagcagcgac atccccaagg
      781 tccgcttgcg tccccacaag tcggccaggc ggccgccggt tgccgctccc aagaaaagtc
      841 caatcgttgc cgcgctgaaa ataacgcctg cctgcgccgc cgacaggccg aactcggcaa
      901 tcacgcgcgg ccccgcgaca cccatcgatt gattatcgaa tccttcgacg agcgcggcgc
      961 cggtgcaaat ggcgagcgcg atgccgggcc gctgtgcgta gtgcggggcc gggtcgttct
     1021 gtggttgtcc tacgatacgg ttaatctgtc ttatgatacg gtattcatca agggtccagc
     1081 aatctcggcc cgcacccctc ctcgcgcaac ttgtcgcccg ggttacgcag cgggcatgct
     1141 ttcatcgaca ggcagccgca gccgatgcag cccgagaggc tgtcgcgcaa tcgcgtgagc
     1201 cggtcgatgc gctcctcgag gtcgcgcttc caaaccgcgg acagccgtcg ccagtctgcc
     1261 gccgtcggtg cgcgcccgtt cggcaaggtc gcgagcgcac ggccgatcgt cgcgagcggc
     1321 atgccgatgc gctgcgcgac cttgatcacc gcgaccaatc tcaaggtttc tcgcgggtag
     1381 cggcgctgat tgcccgggtt gcggcggctc ttgatcaggc ccttggtttc gtagaagtgc
     1441 agcgccgaca ccgcgattcc gctgcgtgcc gcgatctcgc cgacgccgag ttccttgcga
     1501 atcaccgtcg ctgggtcttt cccgcgcatc gtacgcttct cttgacctca agttaacttg
     1561 agcttgtatc gtacctgccg atgcttttca acaacggagc catccgcggc gccacctggc
     1621 gagatctgta cgccgcgggc ctgctcaacc gcttcgccgt actgtgcgcg ggcatctggt
     1681 tgcatgccgt cgacacgctc gtcaccgcca ccatttcgcc ggccatcgtc gaggacatcg
     1741 gcggcgtcgc gttcatcaat tggaccttcc tgttgtacga ggtgggtgcc atcgtggcgg
     1801 gcgccgccac ggcaacgctg tgtcggcgta tcgccatcaa acgcgtgctg gcgtttgccg
     1861 cactgtgcta cgggggcggc tgcctcatcg ccgggtttgc gccgagcatc ggcgttctgc
     1921 tcgcgggccg tctcgtgcaa ggaatcggcg gcggcatgct gctgtcgctg tgctatctcg
     1981 ccattcaagc gtggttcgag ccatgctggt ggagccggtt gtttggcatc gtcgcggtga
     2041 tctggggtgc aggctcgctc ttgggcccct tgatcggcgg catatttgcc ggcctgcact
     2101 cgtggcgaat gacgttcttt gtgtttggca ttcaagccgg cgtgctctgg atcctggcgc
     2161 gcctgtggct gccggcggag acgccggcgc gccgcgaacg ggaggtcttc ccgctcaagc
     2221 cgctgctcac cttgatggtc gcgatcttgc tgatcgccga gagcgcgctc gcgccaacca
     2281 ttcagatatc catcatcggt gtcgtgctcg gactcggtgt cctctactgc gctgcacgcc
     2341 tggacgcgag acttaactcg aaattatttc cggtgcagat cctggatttt cgccatcccg
     2401 ttggcgcagg actgctgatg gtgttcgctc tctccgcggc caccaccggt ttctggacct
     2461 acgggccgct catcctcaag atcctgttcg acacgcaccc gctcgtttgc ggctacatct
     2521 tggcgggcga ggccgtcgcc tggagcttgg ccacgatggc ggtctccaag gttccgattg
     2581 attgcgaccg ttgggtgatc cgcgtcggcg cctcgctggt cgcacttggc gccggcggct
     2641 tcgctctcgc cgtgccggcc gggatgctcg cgggattgat tctctgtgct ctattgcaag
     2701 gggttggctt cggtctttgc tggccctcga tcgtgcaccg cctggcgcga ttctccgcgg
     2761 cggacgaacg cgcgctggcg gtgacttcgc cggagaccgt gcagcggatc ggctacgccg
     2821 tgggcaccgc cgccgtcggc atcgcggcca atctctcggg actcgccgcg gggctgtcga
     2881 tcgccgccgt caaagccgcg gcgttctggg tattcgctgc cttcctgccg gtgttgctgc
     2941 tcgcgctcat gagtgcgtgg aggttcaccc gcgatgccgc cgaggcgtga tcgcgcgctg
     3001 taaacctgcg atcatagctg cgtgaccacc ttcctgctgg cattcggcgc tttgttttcc
     3061 atcgtcaacc ccttgtcggg cgccttcatc tttttcggcg ccacgcgcga actcgatccg
     3121 cgcgtgcgct cacaggtgtc gcgctgggtg gccatttatt cgttctcgat cgtggcagcc
     3181 tcgctctacg tcggcgccta cgtcttgagt ttcttcggca tttccattcc agtgctgcga
     3241 gtggccggcg ggatcgtcgt ggctttatcc ggctggcgga tgttgaacga gcccgactac
     3301 acccagcagc ggcgtagcga aaccccgagc ccgcgctcga tggacgtgcc gccgagccgc
     3361 ctcgcgttct atccgctgac catgccgctc accacggggc ccggtaccat ttccgtcgcc
     3421 atttccttgg gggccggccg gccgcgcggt tttcatgcgc cgttgttgga gttcttcgtc
     3481 gagacgctgg ccgccgtggc gctgctcgcg cttctggtgt acgccttcta tcgaaattct
     3541 gcgcgcctgg ccggattgat gggcgcgacc gggaccacca tcgtggtgcg actctctgca
     3601 tttttattgt tctgtatcgg aatccaggtg ctgtggaatg gggcgtcgga gctgatcggc
     3661 tcgctgccgc tcgggaccgc aggcaccagt taaaggagtg gtcccatgag catcggattc
     3721 gaccggcggc gctttctcgg caccgcaggt gcgctatcgc tgacactgct atcgaaacag
     3781 ttacgcgcgt acgcgacccc ggccgcgccg gtggcaccgg tggcgcgcat cgatgtggtt
     3841 cgcgacacct atttcggtga aacgctgagc gatccttatc gctggatgga aaacgccaat
     3901 gatcctgact ggctgccgtt cctacgcggc cagaacgctc acacgcacgc catactcgac
     3961 gcgctgccga tgcgcgcatc gctcctgcgg cgcatccaag agctgtcgag caatacggtg
     4021 gccaccggcc gggtgcagcg cgcgggcggt ttgacgtttt tcattcagcg gccgcaaggc
     4081 gcggacaatt tcaaattgtt cgtgcgcaaa ggcggccgcg accgcgtgct catcgatccc
     4141 accaaggccg gcgataccac ggggcacacc tcgctcgact ggtggcgggc cgcgccggac
     4201 ggacggcatg tggtgtacgg cctgtcgaag aacggcagcg aggattcgac cttgcgcgtg
     4261 ctcgcggttg cggacggccg cgatctggcc gaacgcatcg gcaacgccca gcaagcgaat
     4321 ccggcatggc tggatgacgg cagcggcttc ttttacaacc agctcaccgc cgcggtcgca
     4381 acgccggagc gttttctcga cagtcaggcg cgcttgcatc gtctcggtac ggatccgaac
     4441 tccgatcctg tgctgatgaa gcgcggtttg gtgcccggtg tcgagtatga gcgcatacag
     4501 gcaccctata tcgtgaccta cccgggtgcg cgtcacgcga tcttgctgct gtcggacgtg
     4561 aggccggaag gccgtgcctt catcgcgccg ctggccgatg tgctcgcggg ccgaccccgc
     4621 tggacgccct ttgccgcctt cgaagacgag gtcacggact tcgaactcga gggcgaagat
     4681 ttgtacctgt tggtcaatca cggcaccgcg cgcggccgca tcgtcaagac ctcggcggcc
     4741 tcgcccaata tcgccaccgc cgccgtggtc gtgccgcaag gcccctatgt catccaggac
     4801 atttcgcgag cgcgcgatgg gctgtatctg cggttgatgg atggcggcct gagccgcctg
     4861 caacgactcg agcacggcgg ccgcatcacg gatatcgccc tgccgttcga gggctcgtta
     4921 gggtcggtgt acaccgcggc cgacgaagac ggcgcactcc tgtcgttgac cggttggctc
     4981 gaacctgccg cgatctactc ggtcagcaca gaagggcgtg tcgccgatac cggcatcacc
     5041 ccgaagccgc ccatcgacgt cgccccttat gtcgcgcggc gtttttttgc aaccgccaaa
     5101 gacggcacga agattcctta tagcgtcgtg catcgcaaag acctcaccct cgacggcagc
     5161 gcgcctgcct ggatcacggc ctacggatcg tatggcctct cgcccaacac gccggcgttc
     5221 gtcggacgtg ctctggcact gatcgacgcc ggtgccgtcg tcggctacgc caacgtgcgc
     5281 ggcggcggcg aatacggccg cgaatggcac aaagccggac agctcgccaa caagcccaat
     5341 acctggcgcg atctgatcgc ggtatgcgag gacctggtgg cgaagaaata tacctcgcca
     5401 tcgcatctgg cgatcggcgg gcgctccgcg ggcggcatca ccgtcggccg cgcgatgacc
     5461 gagcggccgg agctgtttgc cgccgtggtc gacggcgtcg gctggtcgaa cccgctgcgc
     5521 tatgtggtcg agcaggacag ctataccgaa gaacccgaat ggggcgccat cagcaatgcc
     5581 gaaggctatc gattcttgaa gctcatcgac agttatcagt cggtcaagga cggcacgcgc
     5641 tatccggcag tgctcctcac caccggcgtc accgatccgc gcgtcgcacc ctttcacgtc
     5701 gccaagatga ccgcacgact gcaggcagcg agcagttccg gcaaacccat actgctgcgc
     5761 gttgacttcg acgccggcca tggcatcggt tccacgcgtt cgcagcagga ccgcgaggtc
     5821 gccgacaccc acgcgttcct gttgtggcag ctacgcgggt agaggattgc attatgtatc
     5881 gtcgcagtgc atccggcggc tcgcccggac tcgaaaccga cgcggctgcc tcgggaaagc
     5941 aagccc
//
LOCUS       DASWZT010000189         8802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_83567, whole genome shotgun sequence.
ACCESSION   DASWZT010000189 DASWZT010000000
VERSION     DASWZT010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8802
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83567"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VGE92_05025"
     CDS             complement(<1..507)
                     /locus_tag="VGE92_05025"
                     /inference="COORDINATES: protein motif:HMM:NF025221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HEY1313214.1"
                     /translation="MKLKFFVGTLLAALACGGFGSALGADLASLTNADASAGLKKALD
                     QGINQAVGKLGVADGFLKNPQVKIDLPPKLAKAESVMRMVGAGHELDDLVTAMNRAAE
                     AAVPESKVLLKQALHAMTLQDAKRILTGGDDAATQYFKAATYTPLKVKFAPIIHGATE
                     KVKLAETYD"
     gene            complement(600..1895)
                     /locus_tag="VGE92_05030"
     CDS             complement(600..1895)
                     /locus_tag="VGE92_05030"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEY1313215.1"
                     /translation="MRLIFLVCMFATAAAGEEPAAQNFAVHGQFTYVEQETSDFNAPY
                     AGTNSLSPDRGAETTDLTLYLGAHLWRGAEAWIDGEIDQGFGLDDTLGAAGFPSGEAY
                     KIGKDQPYLRLPRLFVRQTFDLDGERAAVAAAANQLGGSHSVNRLEFTIGKFSVADLF
                     DANQYAHDPRADFFNWAAIDAGTFDYAADAWGYTAGAALEWYQGAWTLRGGLFDLSTV
                     PNSVHLDPGFHEFQAVLELARRLEIAALPGSASLTVFDSRGRMALLDEYVQLAATARG
                     AVDIAALRRYRGRIGASLDFAQQLWPDLGVFARIGKAGGNVETYEFTDIDRSESLGIS
                     LQGTHWRRADDSVGLALIDNGISAARERFLNAGGLGILVGDGRLPRPGPEQIIETYYS
                     FSLFAAAHLSVDYQWIDHPAYNRERGPVSLVALRLHAQF"
     gene            complement(1900..2163)
                     /locus_tag="VGE92_05035"
     CDS             complement(1900..2163)
                     /locus_tag="VGE92_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003089094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HEY1313216.1"
                     /translation="MTHKFPAIASRLKRAQGHLAAVIGMLESGRPCLELAQQLHAVEK
                     AIGNAKKELIKDHIHHCMEETSEALPREFRELIKEFQGVTKYL"
     gene            2269..3432
                     /locus_tag="VGE92_05040"
     CDS             2269..3432
                     /locus_tag="VGE92_05040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY1313217.1"
                     /translation="MPINEHGPTARGNAFLWGGAALGLLFLALVWSGGFGLLRGGKKA
                     DSAPDLMVRQGDKITVPEGSALRSRLSVMPAALQSIGGRLLLPGVVESDPARTVSILT
                     PLSGRVVALKVGLGDRVTQGQVLAIVDSPDLGQAYDDDDKAADSARLTQKNLERQEAQ
                     NKIGVASDRDLDQARSDHAQAAAEYTRTEARLKTLGIARGDRASRLLSVSAPVAGSVT
                     TLAVAAGGMINDPTQPIMSVADLSTVWITAMVPEKNVAEVAKNQDAEVKLVAYPDRTL
                     HGKVLFVSAVIEPDSRRNKIRIAFANPDYALKPNMFATVTLTGAAQSQVVLPSSALLM
                     NNDRTSVFVATAPWTFERRDVDTSIEEGSSVAIRSGVAAGEQVVVRGGILLND"
     gene            3425..6517
                     /locus_tag="VGE92_05045"
     CDS             3425..6517
                     /locus_tag="VGE92_05045"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEY1313218.1"
                     /translation="MIDQFVEFCLHRRIAVFILAALCAAYGVYAWETLSVEAYPELGD
                     VAAQVTTQLPGLAAEEVEQLITVPLEREINGTPGLFLMRSSSTFGLSLITVLFRDGYE
                     DYWVRQRLIERINQAVLPAGAAPSLDPVAGPGGEIFRYTLESDTKNLQELSEIQRWTV
                     IPALKSVPGVVDDTNFGGFTTEYQLDLEPSQLQHYGLGITDVINAINNNNANAGGGRV
                     SRGDQSYVIRGVGLIHTLQDIGSIVVTQAGGAPVLVRDLGRTTLSHQEREGILGKDRN
                     PDTLEGIVLMLKYQNPSQTLRGVHAKVAELNAKLASAGVRIVPYSDRDDLVRATIHKV
                     GQTILEGIALVFLILIIFLGSPRSAFVVAVSIPMALVSVFSLLDLTHVSANLLSLGAI
                     DFGILVDGAIVVTEAVLRRREAMPDEELSESEARAATLQVTRPIFFASLIIITAYLPL
                     FSFERAEAKLFTPMAYTMGYALFGALLTALALVPGLAYLAFRKPGRVYHNRWLERLTE
                     RYDRVLHSCIEQPRIIYGVVIFALCAVIALGGTVGRDFLPDLDEGSLWLQVQMPTGLA
                     LNKASDMASDLRRAILKYPEVSYVVTQTGRNDDGTDPWTMSHIEAPVGLKPYDTWPSG
                     ESKKQFVDRLAADLAKELPGYSIGISQPIIDGVNDMVGGAHSPLVAKIYGDDLDQLRR
                     IGAQIVDVLYSIQGTSSASLFQEPPIPQMEIKLDHDKAARYGINMLDVQNVIQTGVGQ
                     APVSTVYVGERSYPLTVRYTQDARNAPAALGNLLVNTAGGVQVPLSQIADIRVRSGES
                     TVTRENGERNLTIRIDNRDRDLTSYLAEAQAKIAAQVHFDRNKIRLTWGGQFENQRRA
                     EARLLVILALVMGMMLIFLTVGIKSFRQALLILGVVPLATLGGLISLHLTGQNLNVAS
                     GVGFIALFGVAVQNGIIMVSNLNRMRKSGLELHEAIMKGATERLRPVLMTATVATLGM
                     LPAAIHTGVGSDVQRGIATVVVGGLAISTVLTLFVLPSMYHVVEHWAEERHGEARWGR
                     "
     gene            6517..7995
                     /locus_tag="VGE92_05050"
     CDS             6517..7995
                     /locus_tag="VGE92_05050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY1313219.1"
                     /translation="MASRLALGVIAGVLLAAASGCAVGPNFKRPPPPAAAGYGNAPET
                     LGDDAQRLVAGMDIPGEWWTLFKSPALDHLVEQALSANPDVGAAQAAVRQAHELYSAQ
                     TTSFFPVVQGSLSGERSEFPTGTLTSPTVASSSTYTLYTAQLTLSYAPDVFGATRRQV
                     EMAKAQFDSSRFQLEATYLTLSSNVVVTAVQEAALRGQIAATQRLLQLQRQLTDSVLR
                     QRSLGTASDLDVLAQQSAQAQTEQTLPPLQKQLGQSRDALTAMLGRLPSDEPEETFRL
                     EELTLPTELPLSVPSKLVEQRPDVRQAEENMHAASAAVGVALANMLPQFSIDADVGSS
                     ALKLGQLFTPYTGFWDAGASLTQTLFDAGALLHKHRAAYAALDQAAAQYRAAVILACQ
                     NVADTLRALQADAAALAASDAADRAAKATFDLAQRQRALGTIGLVALWNAEQAYRQAE
                     LSLVQARANRYADTAALFQSLGGGWWNRKQEPRYEQFGTSGK"
     gene            7967..8710
                     /locus_tag="VGE92_05055"
     CDS             7967..8710
                     /locus_tag="VGE92_05055"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY1313220.1"
                     /translation="MNNLVRRENNGGTATLWLNRPEKLNALTKDVFEALDRHVEAIAR
                     ETRTVGLVVLRGAGANFSAGYDMNEVLAHVKAHAKPHFHSEVIQKIANLPQPVISAVQ
                     GHCSTGALELALAGDLIVASESTQFSDVYARWGLTPVWGLSLRLPHRIGTAKASEMMF
                     TCRSYSGRQAEAMHLANFCFADDRFEAELGALGADILANSWYANQVNKRALIEIDGLS
                     LHDAHALELFKNEGLAPDAAQRIRKFFAG"
BASE COUNT         1617 a         2715 c         2842 g         1628 t
ORIGIN      
        1 atcgtaggtc tcggccaatt ttactttctc ggtggcgccg tgaatgatgg gcgcaaactt
       61 caccttgagc ggcgtatagg tcgccgcctt gaaatattgc gtcgccgcat catcgccgcc
      121 ggtcaagatt cgtttggcat cttgcagagt catcgcgtgc agcgcctgct tcagcaatac
      181 cttgctttcg ggtacggccg cttcggcggc gcggttcatc gccgtgacca gatcatcgag
      241 ttcgtggccg gcgcccacca tgcgcatcac gctctcggcc ttcgcgagtt ttggcggaag
      301 atcgattttc acctgcggat ttttcagaaa accgtcggcg acaccgagct tgccgaccgc
      361 ctgattgatt ccttggtcga gcgccttctt caagcccgcc gatgcatcgg cattggtgag
      421 cgatgcgaga tcggcgccga gcgcgctgcc gaaaccaccg caagcgagtg cggcaaggag
      481 cgttcccacg aagaatttca gtttcatgtc acgaatcccg atgagccttg cggcgaaagt
      541 tcgtgaacta tgcactaggt ttgagcactc gcgccagccc gcgcaacgcc acgccgcctt
      601 cagaactgcg cgtgcagacg tagcgcaacc agcgacaccg ggccgcgctc gcggttatac
      661 gccgggtgat cgatccattg gtaatcgacg ctgagatgcg ccgccgcgaa cagggagaac
      721 gagtagtaag tctcgatgat ttgctcgggg cctggacgcg gcagccgccc gtcgccgacg
      781 agaatgccga ggccgccggc gttcaagaat cgttcgcgcg cggccgagat gccgttgtcg
      841 atcaacgcca gtcccacgct gtcatccgcg cgccgccagt gagtgccttg cagcgatatt
      901 cccagcgact cgctgcgatc gatatccgtg aactcgtacg tctcgacatt gccgccggcc
      961 ttgccgatgc gcgcaaacac gccgaggtcg ggccacaatt gttgcgcgaa atcgagactc
     1021 gcaccgatgc gtccgcgata gcggcgcagc gccgcgatat cgaccgcgcc gcgcgcggtc
     1081 gcggcgagct gcacgtattc atcgagcaat gccatgcggc cgcggctgtc gaagacggtc
     1141 agcgacgcgg agccggggag ggcggcgatt tcgaggcgtc ttgccaattc gagcaccgct
     1201 tggaattcgt ggaatccggg atcgagatgc acgctgttcg gcacggtcga cagatcgaac
     1261 aatccgccgc gcaaagtcca tgcgccttgg taccactcga gcgcggcgcc cgcggtataa
     1321 ccccaggcat ccgccgcgta gtcgaatgtg cccgcgtcga tggcggccca attgaagaag
     1381 tcggcgcgcg gatcgtgcgc gtattgattc gcatcgaaca gatccgcgac gctgaatttg
     1441 ccgatggtga actcgagccg attgacgctg tgcgaaccgc ccaattggtt cgccgcagcc
     1501 gcaacggccg cgcgctcgcc gtcgagatcg aaagtctggc gtacgaacaa tcgcggcaga
     1561 cgcaaataag gctgatcctt gccgattttg taggcttcac cgctcggaaa cccggctgcg
     1621 cccagggtgt catcgagacc gaatccctga tcgatttcgc cgtcgatcca cgcctcggcc
     1681 ccgcgccaca gatgcgcacc caagtacagc gtcaaatcgg tggtctcggc gccgcggtcg
     1741 ggcgacaggc tgttggtgcc ggcgtagggg gcattgaagt cggaggtttc ctgttccacg
     1801 taggtgaatt ggccgtgcac cgcgaaattc tgtgccgccg gctcctcgcc tgcggctgca
     1861 gtggcgaaca tgcagacgag aaagatcagg cgcatgtctc tacaaatact tggtgacccc
     1921 ttgaaactcc ttgatcagct cgcgaaactc gcgcggcagc gcttcggaag tctcctccat
     1981 gcagtggtga atatggtctt tgatgagctc tttcttggca ttgccgatgg ccttctcgac
     2041 cgcgtgcagc tgctgcgcga gttcgaggca tgggcgcccg gactcgagca tgccgatcac
     2101 ggccgcgagg tgaccttggg cgcgcttcaa gcggctggcg atcgcgggaa acttgtgggt
     2161 catttcaaca ccatggcgca tcctatcccc ctagacagga tatgggaaga ggcatagcat
     2221 cgcccacgct ctaactgtca cagagcggca ttattgtagg gaatcgtcat gccgataaac
     2281 gaacatggac ccacggcgcg gggaaatgcc tttctgtggg gcggtgcagc cctcggattg
     2341 ctgttcctcg cattggtctg gagcggcggt tttggcctgc tccgcggcgg caagaaagcc
     2401 gactctgcgc cggacttgat ggtgcggcag ggcgataaga tcacggttcc cgaaggctcg
     2461 gccttgcgca gtcgattgag cgtcatgccg gcggccctgc agtcgatcgg tgggagattg
     2521 ctgctgcccg gggtggtcga atccgacccg gcgcgcaccg tcagcatact gaccccgctg
     2581 agcggtcgcg tggttgcgct caaggtgggc ctcggcgatc gggtgacgca aggccaggtg
     2641 ctggccatcg tcgattcccc ggatctgggg caagcctacg acgacgacga caaggcggcc
     2701 gacagcgccc gcctgacgca aaagaatctc gagcggcaag aggcgcagaa caagatcggc
     2761 gtcgcgtcgg atcgcgattt ggatcaggcg cgcagcgatc acgcgcaggc ggccgccgag
     2821 tacacccgca ccgaggcgcg cttgaagacg ctggggatag cgcgcggcga tcgagcatcg
     2881 cgcctgctgt cggtgagcgc gccggttgcg ggcagcgtca cgaccctggc ggtcgctgcc
     2941 ggcggcatga tcaatgatcc gactcagccc atcatgagcg tggccgattt gagcacggtg
     3001 tggatcacgg caatggtccc ggaaaagaat gtggctgagg tcgccaagaa tcaggatgca
     3061 gaagtcaaac tcgttgccta tccggatcgc accctgcacg gcaaggtgtt gttcgtctcc
     3121 gcggtcatcg agcccgattc gcgccgcaac aagatcagga ttgcgttcgc gaaccccgac
     3181 tatgcgctca agccgaacat gtttgcgacc gtgacattga cgggagcggc gcaatcgcag
     3241 gtggtgctgc cgagctcggc cttgttgatg aacaacgatc gaaccagcgt attcgtcgcc
     3301 acggcgccgt ggacgttcga gcgtcgcgac gtcgacacct cgatcgagga gggatcttcc
     3361 gtcgccatcc gctccggcgt tgccgccggc gagcaggtgg tcgtgagagg cggaattctg
     3421 ctgaatgatt gaccaattcg tcgaattctg cctgcatcga cgcatcgcgg tgttcattct
     3481 ggctgcgctg tgtgccgcct acggcgtcta tgcctgggaa accctctcgg tcgaagcgta
     3541 tcccgagctc ggcgatgtcg ctgcgcaggt cacgacccag ctaccggggc tggccgcaga
     3601 agaggtcgaa cagttgatca ccgtgccgct cgagcgcgag atcaacggca ccccggggct
     3661 gttcctgatg cgttcatcga gcaccttcgg cctgtccttg atcaccgtgt tgtttcgcga
     3721 cggctacgaa gactactggg tgcggcagcg gctgatcgag cgcatcaacc aagcggtgct
     3781 gccggccggc gcagccccgt cgctcgatcc tgtggcgggt ccgggcgggg agatctttcg
     3841 ttatacgctc gagtcggata cgaaaaatct ccaggagctg tccgagatcc agcgctggac
     3901 cgtgatcccg gcgctcaagt ccgttcccgg ggtggtggac gatacgaatt tcggcggctt
     3961 caccaccgag tatcagctcg atctcgagcc gagccagctg cagcattacg gcctcggcat
     4021 caccgatgtc atcaatgcga tcaataacaa taatgccaac gcggggggcg gccgcgtgtc
     4081 gcgcggcgat caaagctacg tgattcgcgg cgtcggactg attcatacgc tgcaagacat
     4141 cggctcgatc gtggtcacgc aggcgggcgg tgcgccggta ttggtgcgcg atctcggccg
     4201 caccacgctc agtcatcagg aacgcgaggg cattctcggc aaggatcgca atccagacac
     4261 gctcgaaggc atcgtgctga tgctcaaata tcaaaacccc tcgcagacgc tgcgcggcgt
     4321 gcacgccaag gtggccgaac tcaatgcgaa attggcgagc gccggcgtgc gtatcgtccc
     4381 gtacagcgac cgcgacgatc tggttcgagc cacgatccac aaggtgggcc aaaccatctt
     4441 agaaggcatt gcgctggttt ttctgatctt gatcatcttc ttgggcagtc cgcgcagcgc
     4501 attcgtggtc gccgtgagca tcccgatggc gttggtgtcg gtgttctcgc tgctcgatct
     4561 gactcacgtg tcggcgaatc tgctgtcgct cggcgcaatc gatttcggga tattggtgga
     4621 cggcgcgatc gtggtgaccg aagcggtcct gcgccggcgc gaggcgatgc ccgacgaaga
     4681 gttgagcgaa agcgaagcga gggcggcgac gctgcaggtc acgcgcccca tcttcttcgc
     4741 cagcctgatc atcatcacgg cgtacttgcc gctgttctcc ttcgaacgcg cggaggcgaa
     4801 gctgttcacg ccgatggcct acaccatggg ttatgcactg ttcggagcat tgttgaccgc
     4861 gctggcgctg gtcccgggac tcgcgtatct cgcgtttcgc aaacccggcc gggtatatca
     4921 caatcgctgg ctcgaacgcc tcactgagcg ctacgaccgc gtgctgcact cctgcatcga
     4981 gcagccgcgc atcatctacg gcgtcgtcat atttgccttg tgcgccgtca tcgccttggg
     5041 aggcacggtg ggccgggatt tcctgccgga tctcgatgaa ggttccttgt ggctgcaagt
     5101 gcagatgccg accggactgg ccttgaacaa ggcgagcgac atggcgagcg atctgcgccg
     5161 cgccatcttg aaatatcccg aggtgtccta tgtggtcacg caaaccggcc gcaatgacga
     5221 cggcaccgac ccgtggacca tgtcgcacat cgaagcgccg gtcgggctga agccgtacga
     5281 cacgtggccg tccggcgagt cgaagaaaca gttcgtcgac cggctggcgg cggacctcgc
     5341 caaggaactt cccgggtatt cgatcgggat cagccagccg atcatcgacg gcgtcaacga
     5401 catggtcggc ggcgcgcaca gtccgctggt ggcgaagatc tacggcgacg atttggatca
     5461 gctgcgccgc atcggcgcgc agatcgtcga tgtgctgtat tcgatccagg gtacctcgag
     5521 cgcctcgcta ttccaggagc cgccgatccc gcaaatggaa atcaaactgg atcacgacaa
     5581 ggccgcccgt tacgggatca acatgctcga tgtgcagaac gtgatccaga ccggagtggg
     5641 ccaggcgccg gtgtccaccg tgtatgtcgg cgagcgatcc tatccgttga cggtgcgtta
     5701 cacgcaagat gcgcgcaacg cgcccgcagc gctcggcaac ttgctggtga ataccgccgg
     5761 cggcgtccag gtgccgctgt cacagatcgc cgacattcga gtgcgctcgg gcgagagcac
     5821 cgtgacccgc gaaaacggcg agcgcaatct caccatccgc atcgacaacc gcgatcgcga
     5881 cctgacgtcc tacctggccg aagcgcaggc caagattgcg gcgcaagtgc atttcgaccg
     5941 caacaagatc cggctgacgt ggggcggcca gttcgagaac cagcggcgcg cggaggcgcg
     6001 gctgctggtg atactcgcgt tggtgatggg gatgatgttg attttcctga ccgtcgggat
     6061 caagtcgttc cgccaagcgt tgttgattct cggcgtggtg cccttggcca cgctcggcgg
     6121 actgatttcg ctgcatttga cggggcagaa tttgaatgtc gcgagcggcg tcgggttcat
     6181 cgcgttgttc ggcgtggccg tgcagaacgg catcatcatg gtgtcgaatc tcaatcgcat
     6241 gcgaaaatcc ggcctggaac tgcacgaggc catcatgaaa ggggcgaccg agcggctgcg
     6301 gccggtgttg atgacggcta ccgtggcgac gctcgggatg ctgccggcgg cgattcacac
     6361 gggagtcggc agcgacgtgc agcgcggcat cgccaccgtg gtggtcggag gactggcgat
     6421 ctccacggtg ttgaccctgt tcgtgctccc ctcgatgtac cacgtcgtcg agcattgggc
     6481 cgaagaacgc cacggcgaag cacggtgggg gcggtgatgg cctcgcgtct cgcgctgggt
     6541 gtcatcgccg gggtactgtt ggccgctgcg tccggctgcg ccgtcgggcc caatttcaaa
     6601 cgcccgccgc cgccggcggc cgctggatac ggcaacgcgc ccgagacgct cggcgacgat
     6661 gcgcagcgcc tggtcgcggg catggacatt ccgggcgagt ggtggacctt gttcaaatcg
     6721 cccgcgcttg accatttagt cgagcaggca ctcagcgcca acccggacgt gggcgccgcg
     6781 caggccgcgg tgcgccaggc gcatgaactc tactcggcgc aaaccaccag cttctttccc
     6841 gtggtgcaag gctccttgag cggcgagcga tccgagttcc cgaccggcac cttgaccagc
     6901 ccgacggtcg cctcgagttc cacgtacacg ctgtacaccg cacaactgac cttgagctat
     6961 gcgcccgacg tgttcggcgc cacgcgacgc caggtcgaaa tggccaaggc gcaattcgac
     7021 agcagccgct ttcagctcga ggcgacctac ctgacgttga gcagcaacgt ggtggtgacg
     7081 gccgtgcagg aggctgcgct gcgcggacaa atcgcggcga cgcagcgcct gttgcaattg
     7141 cagcggcaac tgacggacag cgtgctgcgc caacgctcgc tcggaaccgc gagcgacttg
     7201 gacgttctcg cgcagcaatc ggcgcaggca cagaccgagc aaaccctgcc gccgctgcag
     7261 aagcaattgg ggcagtcgcg cgacgccttg acggcgatgt tgggcaggct gccgtcggac
     7321 gagcccgagg agaccttccg cttggaagag ctgacgctgc ccacggagct gccgctcagc
     7381 gtgccgtcaa agctggtcga gcaacgtccc gatgtgcgtc aggccgagga gaacatgcac
     7441 gccgcgtcgg ccgcggtggg cgttgcgctc gccaatatgc tgccgcaatt cagcatagac
     7501 gccgatgtgg gctcgagcgc gctcaagctc ggccagctgt tcaccccgta cacagggttc
     7561 tgggatgccg gcgcttcatt gacgcagacg ctgttcgacg cgggggcgct gttgcataaa
     7621 catcgtgccg cgtacgccgc cttggatcag gccgccgcgc aataccgggc ggccgtgatt
     7681 ctcgcctgcc agaatgtcgc cgacaccctg cgcgcgctgc aggcggacgc cgcggcgctc
     7741 gccgcgagcg acgcagcgga tcgcgccgcg aaagccacct tcgacctggc gcagcgccag
     7801 cgcgcgctcg gcacgatagg cttggtggcg ctgtggaacg cggagcaggc gtatcgccag
     7861 gcggagctgt cattggtgca ggcccgagcc aatcgctatg cggacacggc cgcgctattt
     7921 cagtcgctcg gcggcggatg gtggaatcgc aaacaggaac ctcgctatga acaatttggt
     7981 acgtcgggaa aataacggcg gtacggcgac tctgtggctg aaccgcccgg aaaagctcaa
     8041 tgcgctcacc aaagatgtgt tcgaggcgct ggatcggcat gtcgaggcca ttgcacgcga
     8101 gacgcgcacc gtcggcttgg tggtattgcg cggcgccggc gcgaatttct ccgcgggcta
     8161 cgacatgaat gaagttcttg cgcacgtcaa ggcgcacgcc aagccgcatt ttcattccga
     8221 ggtcattcaa aagattgcga acctgccgca accggtgatt tccgcggtac agggccactg
     8281 ctcgaccggg gcattggaac ttgcgttggc gggcgatctg atcgtggcct ccgagtcgac
     8341 gcaattctcc gatgtctacg cacgctgggg cttgacgccc gtttggggct tgagtctgcg
     8401 gctgccgcat cgcatcggca ccgccaaggc gagcgaaatg atgtttacct gccgcagcta
     8461 ctccggaaga caggccgagg ccatgcacct cgccaatttc tgcttcgcgg acgatcgatt
     8521 cgaggctgaa ctcggcgcgc tcggcgccga tatcctcgcc aattcgtggt atgccaatca
     8581 agtcaacaaa cgcgcgctga tcgagatcga cggcctcagc ttgcacgacg cgcacgcgct
     8641 ggaattgttc aagaacgaag gattggcgcc ggatgcggcg cagcgcatcc gcaaattctt
     8701 tgccggctaa ttcgagaaca tgcggtcgat tgccgtgaaa tggcgccgcg cgtaggcgag
     8761 aacctctgca tcggtgggat gatcggcgtt ctccacggcg ac
//
LOCUS       DASWZT010000190         5525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_11162, whole genome shotgun sequence.
ACCESSION   DASWZT010000190 DASWZT010000000
VERSION     DASWZT010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11162"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..760)
                     /locus_tag="VGE92_05060"
     CDS             complement(152..760)
                     /locus_tag="VGE92_05060"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY1313221.1"
                     /translation="MNEEASERLAIRELIENWAVWRDAGDWERFATVWHEDGIMMATW
                     FQGPAADFIRVSRQGWARGVSILHFLGGSSIDLAGQRAIAQTKMTISQRAEVHGVRCD
                     VVCTGRFYDFVEKRAGRWGVVLRQPIYEKDRIDPVDPAAKLELNRAALAAFPPGYQHL
                     AYAQTHIGFVVKRDMPQLSGTAVERLYARGKEWLAGGPLALQ"
     gene            848..1660
                     /locus_tag="VGE92_05065"
     CDS             848..1660
                     /locus_tag="VGE92_05065"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY1313222.1"
                     /translation="MSRGKTGTGTIRIGVGGWTYEPWRGTFYPGNLSQKRELEYASRQ
                     LTSIEINGTYYGSQKPATFAKWHDETPDGFVFSVKAPRYAVNRKVLAEAGSTIARFFA
                     SGLMELKDKLGPINWQFLPSKKYDRADFEAFLKLLPKEAAGRVLRHVVEVRHDSFRSA
                     DFIALLREYGVAVVTAGDSSYPQMADATASFVYARIMGTQASEELGYSESALDAWAAR
                     ARAWATGALPPGLDRVERQSADGKPRDVYLYLISGHKLKNPAAAKSLIGRLA"
     gene            complement(1663..2391)
                     /locus_tag="VGE92_05070"
     CDS             complement(1663..2391)
                     /locus_tag="VGE92_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEY1313223.1"
                     /translation="MRNALLGEFAGTLMMMLLGDGVVAACLLKRTKAEGAGWMVITTA
                     WAFAVLCGIFTANLFGSVDAHLNPAITMAFAVKSGDFSKVLPYALAQVSGSFSGAALV
                     WLFYWPHWKVTDDRAAKLAVFCTAPAIRAFGSNLFSEILGAFVLVIVVGAISSKLVLT
                     TGAVAGLTPFLVACLVWSIGLSLGATTGYAINPARDFGPRLAHALLPISGKGGSDWSY
                     SWIPILGPITGASAAGYLLRAMGA"
     gene            complement(2391..3989)
                     /locus_tag="VGE92_05075"
     CDS             complement(2391..3989)
                     /locus_tag="VGE92_05075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEY1313224.1"
                     /translation="MNREEMLQRIASRTEPWDIIVIGGGAVGAGVAVDASSRGFATLL
                     LEREDFGKGTSSRSTKLVHGGVRYLEQGNISLVMEALKERGLLLRNAPHIVHDLPFVV
                     PNYSWWEAPFYGIGMKVYDLLAGKYNFGRSRVLSLEETLERLPTIQREGLRGGVLYHD
                     GQFDDTRLLVHLLATAVDHGATVVNYVEAVDFVKDAQGFVGGVILEDRETQTRHTAHA
                     KVTVNATGIFTDETRRLAEAGAESMVTPSQGIHLVLDRTFLNGDSAIMVPRTSDGRVL
                     FAIPWHNRTLVGTTDTLIDAPCYEPLPLEEEIEFVLTTASDYLSRPASRSDVLSIYVG
                     IRPLVSAPGGGVGKTSALSRGHTIHVDNSGLMSIVGGKWTTYRHMAEDCVDHAITLGS
                     LPDTACVTADLPIHGYHTQTEQFGALGVYGSDAASIRALVDDNPQLGERLHPELPYIA
                     AEIVWSARHEMSRTLDDALARRTRALLLNARAAVAVAPRVAALLAQELGADERWIKSQ
                     VASFTALARQYILEPAAQTTGSCV"
     gene            complement(3986..5482)
                     /gene="glpK"
                     /locus_tag="VGE92_05080"
     CDS             complement(3986..5482)
                     /gene="glpK"
                     /locus_tag="VGE92_05080"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEY1313225.1"
                     /translation="MAQYVLSLDQGTTSSRAMLFDHGGKVLGIAQHEFAQHFPRTGWV
                     EHDPLDILTSQLNAAVEVLARAQIRPRDVVALGITNQRETTIVWDRKTGTPVYNAIVW
                     QDRRTADMCRQLREAGHEEPLRRKTGLLIDPYFSGTKIAWILDNVAGARQRAEQGQLA
                     FGTVDSWLIWNLTSGKRHVTDRTNASRTMLYNIVDDQWDKELLQLFNIPEGILPEVVW
                     SSEKVSQVSTSLGLADIEIAGIAGDQQAALFGQLCVNPGDAKSTYGTGCFLLQNIGES
                     FALSNQKLITTVACSTDRKLHYALEGSVFIGGAVVQWLRDKMQFFDKAADIEKVAAAV
                     ADSSNVVLVPAFTGLGAPHWDPYASGMLIGLQRDTEIGHIARAALESIAFQVTDVILA
                     MQSDTGVQSTALKADGGAAANDLLMQFQADVLGIPVERPAILETTAQGAAYLAGLASG
                     FWSDVEEIAKTRPAGQVFKPAGNRDRALLQYRRWQDAVARSKGWNTPG"
BASE COUNT         1067 a         1722 c         1712 g         1024 t
ORIGIN      
        1 ggcttgcgct cgctgcgggt atcggcatcg cgcccgggcc gatattttct gcccgccagc
       61 aattcggcaa tttcattcgc atcaacaccg gtcatccgtg gacttcgacg ctcgataagg
      121 cggtgcagcg cctcggagaa atcttgcggc gctattgaag agcgagcggg ccgccggcca
      181 accattcctt gccgcgcgca tacaagcgct ccacggcagt tccgctgagc tgcggcatgt
      241 cgcgtttgac gacgaagccg atgtgcgttt gggcgtaagc caaatgttga tagcccggcg
      301 gaaaggccgc gagggccgcg cggttcaatt cgagcttggc ggcaggatcg accgggtcta
      361 tccgatcctt ctcgtagatc ggctgacgca gcaccacgcc ccagcggccg gcgcgctttt
      421 cgacgaagtc gtagaaacgg ccggtgcaca caacgtcgca gcgcacgccg tgcacttcgg
      481 cgcgctgcga gatggtcatc ttggtttgcg cgatggcgcg ttgacccgcc aagtcgatcg
      541 atgagccgcc gagaaagtga agaatgctca cgccccgcgc ccagccctgc cggctcacgc
      601 gaatgaaatc cgcggcggga ccctggaacc aagtcgccat catgatgccg tcttcatgcc
      661 agacggtggc gaatcgctcc cagtcgcccg catcgcgcca taccgcccaa ttttcaatca
      721 gttcgcgaat cgccaagcgc tcgctcgctt cctcattcat ccaagatctc ctgtcgggtc
      781 cgggcgaaca gtggctccgg ccgtcgaatt gcattatatg tagcgacggc ggccagtgag
      841 gaactcaatg agtcggggca aaacgggcac aggcacaatt cgcatcggcg tgggtggttg
      901 gacctacgag ccctggcgcg gcacgttcta tccgggaaac ctgtctcaga aacgagagct
      961 cgaatacgcc agccgccaat tgacgtcgat cgagatcaat ggcacgtact acggctcgca
     1021 aaagccggcg accttcgcca aatggcacga cgagactccg gacggctttg tcttttcggt
     1081 gaaagccccg cgttatgccg tgaatcgcaa agtgctggcc gaggccggca gcaccattgc
     1141 tcgattcttc gcaagcggtc tgatggagct caaggacaaa ctcggcccca tcaattggca
     1201 gttcctgccc agcaagaaat acgatcgcgc ggatttcgag gccttcttga agcttctgcc
     1261 gaaggaagcg gcaggccggg tgttgcgcca tgtggttgag gtgcggcatg acagctttcg
     1321 ttcggccgat ttcattgcgc tgctgcgcga gtacggcgtc gccgtggtga ccgcgggcga
     1381 ttccagctat ccgcaaatgg cggatgccac cgcgtccttc gtctacgccc gcatcatggg
     1441 tacgcaagcg agcgaagaat taggctattc cgagagcgca ctcgatgcct gggcggcgcg
     1501 ggcacgcgcc tgggccaccg gtgccttgcc gccggggctt gatcgcgtgg agcggcagag
     1561 cgcggacggc aagccccgcg acgtctatct ctatttgatc agcggccaca aattaaaaaa
     1621 ccccgcggca gcgaaatccc tgattgggcg cctcgcctga gctcaggcgc ccatcgcccg
     1681 caacagatat ccggccgcag aggctccggt gatcggcccg aggatcggta tccaggagta
     1741 ggaccaatcc gatccgccct tgccggatat cggcagcagc gcgtgcgcca accgtgggcc
     1801 gaaatcgcgg gccggattga tcgcataccc ggtggtcgcc ccgagagaca gaccgatgga
     1861 ccacaccagg cacgccacca gaaatggagt caaaccggca accgcacccg tcgttagaac
     1921 cagtttggaa ctgatggcgc cgacgacgat gaccaggacg aaggcaccca ggatctcgct
     1981 gaacaggttg gaaccgaagg cgcgaatggc cggcgctgtg cagaacaccg caagcttggc
     2041 ggctcgatcg tcggtgactt tccaatgtgg ccagtagaat agccagacga gcgcggctcc
     2101 gctaaagctg ccgctcactt gagcaagcgc ataaggaagc accttggaga aatcgccgct
     2161 cttgaccgca aaggccatgg tgatggcggg attgagatgc gcgtcgacac tgccgaacag
     2221 gttcgcggta aaaatgccgc acagcacggc gaatgcccag gctgtagtga tgaccatcca
     2281 gcctgcgcct tcggccttgg tccgtttgag aaggcatgcg gcgacaacgc cgtcgccgag
     2341 caacatcatc atgagcgtgc cggcaaattc tccgagtagc gcatttcgca tcacacgcag
     2401 cttccggttg tctgtgccgc gggctcgagg atgtactgcc gcgccagcgc ggtgaagctc
     2461 gcgacttgag atttgatcca gcgttcgtct gcgcccagtt cttgggcaag cagggccgcc
     2521 acgcgcggcg ccacggcgac ggccgcacgc gcattgagta ataacgcgcg cgtgcgccgc
     2581 gcaagtgcat cgtcaagtgt ccgtgacatc tcgtgccgcg ccgaccagac gatttccgcc
     2641 gcgatgtacg gcagctccgg atgcagcctc tcgccaagtt gcggattgtc atcgacgaga
     2701 gctcggatgc ttgcggcgtc gctaccgtag acaccgagag cgccgaactg ttcggtctgg
     2761 gtgtgatagc cgtggatcgg cagatcggcg gttacgcacg cggtgtccgg caggctcccg
     2821 agcgtgatgg cgtgatccac gcaatcctca gccatgtgcc ggtaggtggt ccacttgccg
     2881 ccgacgatac tcatcaaccc tgagttatcg acgtgaatcg tgtggccgcg tgacaaagcg
     2941 gacgtcttgc cgacaccgcc gccgggtgcg ctgacgagcg gtcgaattcc gacgtaaatg
     3001 ctcagaacgt ccgagcgtga cgcgggccgg ctcaaatagt cgctcgcggt ggtgagcacg
     3061 aactcgatct cctcctcgag aggcaagggc tcgtaacagg gcgcatcgat gagcgtatcg
     3121 gtggtcccga ccaaagtccg gttatgccag gggatggcaa acagcacccg tccatcgctg
     3181 gtgcgcggca ccatgatcgc gctgtcgccg ttcaagaagg tgcggtcgag aaccaggtga
     3241 attccctggc tcggggtcac catcgattcg gcgccggctt ccgccaagcg tcgggtctcg
     3301 tcggtgaaaa tcccggtggc attgacggtc actttggcat gcgcggtgtg gcgcgtctgc
     3361 gtctcacgat cttcgagaat gacaccgccg acgaagcctt gtgcgtcctt cacgaaatcg
     3421 accgcttcga catagttcac gacagtcgcc ccgtgatcga ccgccgtcgc cagcaaatgg
     3481 acgagaagac gcgtgtcgtc gaactgcccg tcatgataga gaaccccgcc tcgcaagccc
     3541 tcgcgttgga tcgtcggcag ccgttccagg gtttcttcga gtgacaggac ccgcgatctc
     3601 ccaaaattgt atttgccggc cagtaggtcg tacaccttca ttccgatgcc gtagaacggc
     3661 gcctcccacc aggaatagtt cggaaccacg aagggtagat cgtgaacgat gtgcggcgcg
     3721 ttgcgcagca acagcccgcg ctccttcaag gcctccatga ccagggaaat gttcccctgc
     3781 tcgagataac gaactccgcc atggaccagt ttggtcgaac ggctcgaggt gcctttgccg
     3841 aagtcttcgc gctcgaggag aagcgtggca aagccgcgcg aactcgcatc caccgccact
     3901 ccggcaccga ccgctccgcc gccgataacg atgatgtccc agggctcggt gcggcttgcg
     3961 atccgctgca gcatctcctc acggttcatc caggtgtgtt ccaacccttg gaccgggcga
     4021 cggcatcctg ccagcggcgg tactgcagca gcgcccggtc gcgattgccg gcgggcttga
     4081 agacctggcc cgccggacga gtcttggcaa tttcctcgac atcgctccaa aatccgctgg
     4141 ccaagccggc cagatacgcg gcgccttggg cggtggtttc gaggatggcc gggcgctcga
     4201 ccgggatgcc gagcacatcc gcctgaaatt gcatgagcag gtcattggcc gcggcacccc
     4261 catcggcctt gagtgcggtg gattgcacgc cggtatcgct ctgcatcgcc agaatgacgt
     4321 cggttacttg gaacgcgatg ctctcgagcg cggcgcgcgc gatgtggccg atctcggtgt
     4381 cgcgttgcaa accgatgagc atgccgctcg cgtaaggatc ccaatgaggg gcgcccaagc
     4441 ccgtgaatgc cgggaccagg acgacattcg aggaatcggc gaccgctgcc gccaccttct
     4501 cgatatcggc ggccttatcg aaaaactgca ttttgtcacg cagccattga acgaccgcac
     4561 cgccgatgaa cacgctgcct tcgagcgcgt aatgcaactt gcggtccgtg ctgcacgcga
     4621 ccgtggtgat gagcttctga ttggaaagag caaagctctc gccgatattc tgcaacaaga
     4681 agcaaccggt accgtaggtg ctcttcgcgt caccgggatt gacgcatagt tgaccgaaca
     4741 gcgccgcctg ctgatccccc gcaatgccgg cgatctcgat atcggcgagg ccaagcgagg
     4801 tgctgacctg cgacactttc tcgctcgacc agacgacctc gggcaagatg ccctcgggga
     4861 tattgaagag ctggagcagt tccttgtccc actgatcgtc gacgatgttg tagagcatcg
     4921 ttcgggaagc attcgtccga tcagtgacgt gccgcttgcc gctggtgaga ttccagatga
     4981 gccagctgtc gacggtgccg aaggccagct gaccctgctc agcgcgctgg cgggcgccgg
     5041 cgacgttgtc caatatccag gcgattttcg tccccgaaaa atacggatcg atcagcaacc
     5101 cggtcttgcg ccgcagcggc tcctcgtggc ccgcctcgcg caattggcgg cacatatcgg
     5161 cggttcggcg gtcctgccaa acgatggcgt tatagacggg ggttccggtc ttgcggtccc
     5221 atacgatcgt ggtttcacgc tggttggtga tgccgagcgc gaccacatca cggggtcgga
     5281 tctgcgctcg cgccaagacc tcgaccgccg cattgagctg gctcgtcagg atatcgaggg
     5341 gatcgtgctc gacccacccc gtgcgcggga agtgctgggc gaattcgtgc tgggcaattc
     5401 ccagcacctt gccgccgtgg tcgaacaaca tcgcgcggga actggtggtt ccctggtcga
     5461 gtgaaaggac gtattgcgcc attggctatt ttttctgagt ggccgtgccg agagcttacc
     5521 ccgcc
//
LOCUS       DASWZT010000191         3644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_45007, whole genome shotgun sequence.
ACCESSION   DASWZT010000191 DASWZT010000000
VERSION     DASWZT010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45007"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VGE92_05085"
     CDS             complement(<1..661)
                     /locus_tag="VGE92_05085"
                     /inference="COORDINATES: protein motif:HMM:NF023391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016851 - magnesium chelatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3479 domain-containing protein"
                     /protein_id="HEY1313226.1"
                     /translation="MPKRITAAESTPLRFTIVTMDSHLSSATARAATRLAKEMPGLSL
                     SIHAADEWGNDPEALQRCLNAIARADIIVVTMLFMEDHFQPLLKALQERREHCDAMLC
                     AMSAAEVVRLTRLGRFSMDGKQSGPLALLKRLRGAHKNTRSAGADQMKMLRRIPQFLR
                     FIPGTAQDVRAYFLTLQYWLAGSEENVGNMVRFLVRRYASGARACLRSTARVAPPLDY
                     PE"
     gene            complement(636..2174)
                     /gene="bchB"
                     /locus_tag="VGE92_05090"
     CDS             complement(636..2174)
                     /gene="bchB"
                     /locus_tag="VGE92_05090"
                     /EC_number="1.3.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin:protochlorophyllide reductase
                     (ATP-dependent) subunit B"
                     /protein_id="HEY1313227.1"
                     /translation="MQLTVWTYEGPPHVGAMRIATAMRDVHYVLHAPQGDTYADLLFT
                     MIERLPKRPPVTYTTFQARDLGGDTAGLLQRSVREAYERFTPRAMLIGASCTAELIQD
                     DPGGLARALKLPIPVVVLELPSYQKKENWGAAETFYQLVRAICTPLAPAPGFRRPPRA
                     PGARLSCNLLGPTALGFRHRDDLLAVRQILEQLQVDINVIAPLGASPADLQRLGAADF
                     NVLLYPETAGVAAQWLSRTFDQPTIKSIPIGVGATREFIAEIAALSGVDPKGAANHPA
                     EHASWYSRSVDSTYLTGKRVFVFGDATHAVAAARIASEELGFAVVGLGTYSREYAREV
                     REAAQRYSLEALITDDYLEVEAKVAELQPELVLGTQMERHIAKRLGIPCAVISAPVHV
                     QDFPARYSPQMGFEGANVIFDTWVHPLMMGLEEHLLTMFRGDFEFNDAAPASHLGSSA
                     VVAQQSAALASLWAPDAEKELGKIPFFVRGKARRNTEQFAAERRVALITVETLYDAKA
                     HYSR"
     gene            complement(2177..3424)
                     /locus_tag="VGE92_05095"
     CDS             complement(2177..3424)
                     /locus_tag="VGE92_05095"
                     /EC_number="1.3.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin:protochlorophyllide reductase
                     (ATP-dependent) subunit N"
                     /protein_id="HEY1313228.1"
                     /translation="MSAVRISSERGQREVFCGLTGIIWLHRKIQDAFFLVVGSRTCAH
                     LIQSAAGVMIFAEPRFATAIIDERDLAGLADANEELDRVATQLLARRPDIKLLFLVGS
                     CPSEVIKLDLSRAALRLSARFSPHVRVLNYSGSGIETTFTQGEDACLASLVPSLAARP
                     PTAPANLLIVGALADVVEDQIRRLFDDLGIAEVRFFPPRRSTDLPQVGPQTRFLLAQP
                     FLADTARALEERGARLIAATFPLGAEGTLAWLKAAADVWQVPEAVFSRSTQPAYERAQ
                     RSLMPYRDQLAGKRIFFFPDSQLEVPLARFLSSELCMRPIEIGTPFLHRAHLAADLAL
                     LPADAYVVEGQHVENQLDRCHAARPDLVVCGLGLANPLEAAGLTTKWSIELVFTPIQG
                     FNQAADLAELFSRPLVRRARLVA"
     gene            complement(3421..>3644)
                     /locus_tag="VGE92_05100"
     CDS             complement(3421..>3644)
                     /locus_tag="VGE92_05100"
                     /inference="COORDINATES: protein motif:HMM:NF018935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0019685 - photosynthesis, dark reaction
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-vinyl bacteriochlorophyllide hydratase"
                     /protein_id="HEY1313229.1"
                     /translation="LHTAYLGALLFGSLHARYQMYIALLAYSAYLLNATQFLVKLRAA
                     RLERSPQSNGAGPAARASGSHPAAPGVIA"
BASE COUNT          703 a         1142 c         1189 g          610 t
ORIGIN      
        1 cctctggata gtcgagcggc ggcgccactc tcgccgtcga ccgcaggcag gcgcgtgctc
       61 cggatgcgta gcgccgcacc aggaagcgca ccatgttccc cacgttctcc tctgaaccgg
      121 ccaaccaata ttgcagagtc aggaaatagg cgcgaacatc ctgcgcggtg cctggaatga
      181 atcgcaggaa ttgcggaatc cgccgcagca ttttcatttg atcggcgccg gcgctgcggg
      241 tgttcttgtg cgctccgcgc aagcgtttga gcagcgccag cggtccgctt tgcttgccgt
      301 ccatgctgaa gcggccgagc cgcgtgagac gcacgacctc cgccgcagac atggcgcaca
      361 gcatggcgtc gcagtgttcg cggcgttcct gcagtgcctt gagcagcggc tggaaatgat
      421 cctccatgaa cagcatcgtc accacgatga tgtcggcgcg cgcgatggcg ttcaagcaac
      481 gctgcagcgc ctcgggatcg ttgccccatt cgtccgcggc gtgaatgctg agcgacagac
      541 ccggcatctc cttggcgagc cgcgtcgcgg cgcgcgcggt ggcgctcgac agatggctat
      601 ccatcgtcac gatggtgaag cgcagcggcg tcgactcagc ggctgtaatg cgctttggca
      661 tcgtacagag tctccacggt gatgagcgca acgcggcgct cggcggcgaa ttgctcggta
      721 ttgcggcgcg ccttgccgcg tacgaaaaac ggaatcttgc cgagttcctt ctcggcgtcg
      781 ggagcccaca gcgaggcgag cgctgcgctt tgctgcgcaa ccacggcgct cgatcccaga
      841 tgcgaggcgg gggcggcatc gttgaactcg aagtcgccgc gaaacatcgt cagcaggtgc
      901 tcttccaaac ccatcatcaa agggtgcacc caggtgtcga aaatcacatt ggcgccctcg
      961 aagcccatct gcggcgagta gcgcgccgga aaatcctgta catgcacggg cgcggaaatg
     1021 acggcgcagg gaatgcccaa tcgcttggcg atgtgacgtt ccatctgcgt tccgagtaca
     1081 agttcgggtt gcagctcggc gactttggcc tcaacttcca ggtagtcgtc cgtgatcagc
     1141 gcttcgagag aatagcgctg cgccgcttcg cgcacctcgc gggcatattc gcggctataa
     1201 gtccccaagc cgacgactgc gaagccgagt tcttcgctcg cgatgcgcgc cgcggcgacg
     1261 gcgtgcgtgg catcgccaaa aacgaaaacg cgtttgccgg tgaggtaggt cgaatcaacc
     1321 gagcgcgaat accagctcgc atgctcggca ggatgattcg cggcaccttt gggatcgacg
     1381 cctgacagcg cggcgatttc cgcgatgaac tcgcgggtcg caccgacccc gatggggatg
     1441 ctctttatcg tcggttggtc gaaggtgcgg ctcagccact gcgcggccac gccggcggtt
     1501 tcaggataga gcagcacatt gaaatcggct gcgccgagac gctgcaaatc agctggtgag
     1561 gcgcccaacg gagcgatcac attgatgtcg acttgcaact gctcgagtat ttgccgcacc
     1621 gcgagcaggt cgtcgcgatg acgaaatccg agtgcggtcg ggcccaacag gttgcagctg
     1681 agccgcgcgc ccggcgcccg cggcggccgc ctgaagccgg gagccggtgc cagcggcgtg
     1741 catatcgcgc gcaccaactg atagaaggtc tccgccgctc cccagttctc tttcttttga
     1801 tacgacggca gctccagaac gacgacgggt atcggcagct tgagcgcccg cgccaggccg
     1861 ccgggatcat cttgaatcaa ctcggctgta cacgaggcgc cgatcagcat ggcccgaggc
     1921 gtgaaccgct cgtacgcttc gcgcaccgac cgttgcaaca atccggccgt atcgccgccc
     1981 agatcgcgcg cctgaaaggt cgtgtaggtc accgggggac gtttcggcag ccgttcgatc
     2041 atcgtgaaca gaagatcggc gtaggtgtcg ccctgcggcg cgtgcaatac gtagtgcacg
     2101 tcgcgcatcg cggtcgcgat acgcatcgcg ccgacgtgcg gcggtccttc gtatgtccag
     2161 acggtgagct gcatggctag gcgaccaacc gtgcgcgccg caccaagggc cgcgaaaaga
     2221 gctccgccag gtcggcggcc tggttgaagc cctggatggg tgtgaacacc aactcgatcg
     2281 accatttggt ggtaaggccg gccgcctcca ggggattggc gagcccgagg ccgcacacca
     2341 cgagatcggg acgggccgca tgacaacggt ccagctgatt ctcgacgtgc tgtccttcca
     2401 ccacgtaggc gtccgccggc agaagcgcga gatcggccgc caaatgcgcg cggtgcaaga
     2461 agggagtgcc gatttcaatc ggccgcatgc acagctcgct cgagagaaat ctcgccaagg
     2521 gcacttcgag ctgcgaatcg ggaaagaaga agatgcgctt tccggcgagc tgatcccggt
     2581 aaggcatgag tgagcgctgc gcccgttcgt acgcgggctg cgtcgaccga gaaaacaccg
     2641 cttcgggcac ctgccacacg tcggccgccg ccttgagcca agcgagcgtc ccctcggccc
     2701 ccagcggaaa agtggccgca atcagccgcg cgccccgttc ctcgagggcg cgggcggtgt
     2761 cggctagaaa cggttgcgcc agcaggaagc gcgtctgcgg acccacttgc ggcaagtcgg
     2821 tcgagcgccg cggcgggaag aagcgcacct cggcaatgcc caggtcatcg aacaggcgcc
     2881 gtatttgatc ctcgacgaca tctgccaggg cgccgacgat cagcaggttg gcgggagccg
     2941 ttggcgggcg ggccgcaagg ctcggtacca gcgatgcaag acaggcatcc tcaccctgcg
     3001 taaatgttgt ttcgattccg ctgccggagt aattgaggac ccgcacgtgc ggcgagaaac
     3061 gcgccgacag gcgcagcgcc gcgcgtgaaa gatcgagctt gatgacctca gacgggcagg
     3121 atcccaccaa gaacaacaat ttgatgtctg gacgacgcgc caagagctgg gtcgcaaccc
     3181 gatccagttc ctcgttggcg tcggccaggc cggccagatc gcgctcatcg atgatggcgg
     3241 tcgcaaaacg cggttcggcg aaaatcatca cgccggccgc ggattggatc aggtgggcgc
     3301 aggtgcgcga accgaccacc agaaagaatg cgtcctggat ttttcgatgc agccagatga
     3361 tgccggtcag gccgcaaaac acctcgcgtt ggccgcgttc gctggagatt cgcacggcgc
     3421 tcacgcgatg actccaggcg ccgcggggtg cgagcccgac gcgcgggccg cagggccggc
     3481 gccgttcgac tgcggggatc gttcaagccg agccgcgcgc agtttcacta aaaactgcgt
     3541 tgcgttcagc aaataggcag aataggctag caacgcgatg tacatttgat agcgggcatg
     3601 caacgagccg aataacaacg cgcccagata cgcggtgtgt aacg
//
LOCUS       DASWZT010000192         7406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_78857, whole genome shotgun sequence.
ACCESSION   DASWZT010000192 DASWZT010000000
VERSION     DASWZT010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7406
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78857"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VGE92_05105"
     CDS             <1..592
                     /locus_tag="VGE92_05105"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HEY1313230.1"
                     /translation="LTLVLVTVWALRLSFYITVRNWGHGEDRRYVAIRARNEPNFPLK
                     SLYLVFALQAGLAWIISLPLLAGILSDAPWGAFDVLGAAFWVVGFSFEAGGDWQLARF
                     KSDPANHGQVMDRGFWRFTRHPNYFGDFCVWWGFYLIAASAGAWWSIIAPLLMSVLLM
                     RVSGVTLLEKDIGERRPKYADYVRRTNAFFPGLPKR"
     gene            597..2078
                     /locus_tag="VGE92_05110"
     CDS             597..2078
                     /locus_tag="VGE92_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY1313231.1"
                     /translation="MSASYEPMVSEAMEFERQGRVPEAIEAYQRVLAGWPGLPNCWYN
                     LGLLQRKSGDYSAALASYQEALERGVPQPEEVHLNRGVIFSDCLHQYDAAERELKRAL
                     ALNPAYAPALVNLANLHEDLGRRDQAADAYERILALAPDDPQVLARYAGLKSFTSADD
                     PMIARLRAVRGRPGLSAAGRASVEFALGRALDACGAYDAAFEAIRAANRHSRESAGLA
                     FRDYDLKAQEQFVDRLIEAFPAGLRVAGPRMMSLRTVPPQPIFVCGMFRSGSTLTEQL
                     LAGHSQVKAGGELDFLPRAVQTQLAPFPESMQTVSQFRLETLAAEYLQLLRDVFPQAT
                     YVTDKRPDNFLYIGLIKLLFPNAKIVHTTRNALDNCLSIYFLHLDQGMSYALDLMDIG
                     HFYGQYLRLMAHWKTLFGADILDLSYDSLVSEPQPVMRGLLQSLDLPWDEHCLSVPSA
                     GRAIRTASVWQVREPLYQRSSGRSRNYERHLQVLREYLALQSR"
     gene            2565..5591
                     /locus_tag="VGE92_05115"
     CDS             2565..5591
                     /locus_tag="VGE92_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1313232.1"
                     /translation="MGNNRKLQVAVRAALALATASAAIPAAEAQTATAANNEATTGAL
                     EEVVVTGSRIQSTNLVSISPVTTVTAEAIQQTGLTRVEDILNNLPQVFAGQGSNVSNG
                     SDGTATVDLHDLGPQRTLVLVNGRRLGPGGGATANASDINQIPAQLIEKVEVLTGGAS
                     ATYGADAVSGVVNFILNTHFEGVKIDANYGLYQHNNHDGFAAGVADAQGDPVTQPGSV
                     DAGFNKDLAVLMGSNFADGKGNATAYVTYTNQAPVLEGRYDYSACTLEGKRTSATTTD
                     VSCGGSYTSRGGAFLGYNAAFSKNTVYDTVDPKTGIFRPFNTATDLFNYGPINYYQRP
                     NERWTAGSFINYDVNDHANVYYELMYMKNNSIAQIAPGGDFGNPATINCGPVGVGLPG
                     NPLLTAQERSVICTPANIAAQAPGSPPNTVLLNYILRRNVEGGGRQAAFQNESYRNVL
                     GVKGDFAEAWKYDAYAQFNHVTTYQNNLNYFSNSAIDNALDVVEGPTGPTCESVINGT
                     DPACVPYNIWVPGGVTKAALAYLNIPNSITGFSSEYAAHADVSGDLGKYGVQIPTAKS
                     GMQVNFGAEWREDTVQTDPDYATQQGLASGGAGAIPPLSGQIHVAEVFTEFNLPIMDS
                     IPGADQLALEGGYRYSSYNLGFKTNTFKIGLEWAPVSDIRFRGGFNRAVRAPNIGELY
                     SSPSIGPDGTIDPCWGTPVYTAAECARTGVTAAEYGKIGVNSAYQINTSSSGNPNLTP
                     EKANTTSFGFVFQPSFFTGFSASLDYYDIKITNAIVSSTGVATAIISECALTGSAQAC
                     NLIHRSADGSLWESSAGFVDTPTSNVGVISTKDVDLKMHYSTGIGNAGNLAFNLEGTY
                     VINNITTPFPGAPSFDCAGLWGPTCGNPLPKWRHIFSTDWATPWSGLDLNLRWRYFGA
                     SNVETSSGSPDLAFITTDYPGFNRISNYSYIDTSAAMEVAKGITVRLGINNLLDKDPP
                     TVLSGNCAAGPCNGNTFSQSYDVLGRYVYLHVAAKF"
     gene            complement(5682..6107)
                     /locus_tag="VGE92_05120"
     CDS             complement(5682..6107)
                     /locus_tag="VGE92_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313233.1"
                     /translation="MRSINSLLILLSIGGVASQALAADPPSPPAQNATAAATPAAPAV
                     SSASTSPATPAAAPTAAKTDAATSGSTETQVKTLRAAGYKPETHNGQTLWCRREAQIG
                     TRFETKSCGTADDLERQTKESQDMTARVQQKNVFNPKSN"
     gene            complement(6198..6743)
                     /locus_tag="VGE92_05125"
     CDS             complement(6198..6743)
                     /locus_tag="VGE92_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313234.1"
                     /translation="MRRCTSRSILAALILGLGSATPGFADDAAALIGKDLARCAAISS
                     RDERLACYDALAANHGATANAPAGAPAPTAAAAPPAAAVPPAAAVPPKKEDFGLSAAQ
                     LQKVNPQKQIESISAKVTSVWHSGSGHMLLELDNGQSWQLDSEDPLLAAGNTVTIQRG
                     ALGSFLLTTPTRRTHHAHRMH"
     gene            complement(6740..>7406)
                     /locus_tag="VGE92_05130"
     CDS             complement(6740..>7406)
                     /locus_tag="VGE92_05130"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEY1313235.1"
                     /translation="PTFMYFPEIPAIEFFERADFPWLDAIEAATEDIRSEVTNVLIAD
                     RAGLQPYIDYPAGLPIDQWKELNRSRRWSAYFLWNQGTAQPAHIARCPRTAAALAHAP
                     RCEVAGRAPTAFFSILDASTTIPAHTGVTNTRLTVHLPLIVPPDCRFRVGGTTREVRA
                     GQAWVFDDTIDHEAWNGSDMPRAILIFDIWNPFLSEAERDMVRAATEVVVSYYQMPAE
                     SFR"
BASE COUNT         1407 a         2228 c         2293 g         1478 t
ORIGIN      
        1 cctcacgctc gtgctcgtca ccgtctgggc gctgcggctt tctttctaca ttacggtgcg
       61 caattggggt cacggcgagg atcggcgtta cgtcgcgata cgcgcccgga acgagccaaa
      121 ttttccgttg aagagcctgt acctcgtgtt tgcattgcaa gcgggactcg cctggataat
      181 ctcgctgccg ctgctcgctg ggatcttgag cgatgccccg tggggcgcgt tcgatgtcct
      241 cggggcggcc ttctgggtgg tgggattcag tttcgaggcg ggaggcgact ggcagctggc
      301 gcgattcaaa tcggatccgg ccaatcacgg ccaagtcatg gatcgcggat tttggcgctt
      361 tacgcgacat ccgaattatt tcggcgactt ctgtgtgtgg tggggtttct atttgattgc
      421 cgcgtccgcc ggtgcgtggt ggagcatcat cgcgccgctg ctgatgtcgg tattgctgat
      481 gcgcgtttcc ggagtgacac tcctcgagaa agacatcggc gagcgcaggc ccaagtacgc
      541 cgactacgtg cgccgtacca acgctttctt tccgggtttg ccgaagcggt gatgatatga
      601 gcgcaagcta cgagccgatg gtgagtgagg cgatggagtt cgaacgccag gggcgcgtgc
      661 cggaagcgat cgaggcgtac caacgcgtac ttgccggctg gccgggtttg cccaactgct
      721 ggtacaacct cggacttcta cagcgcaaga gcggcgacta tagcgccgcc ttggcttcct
      781 accaggaagc cctggaacgc ggcgtcccgc agccggaaga agtgcatctg aaccgcgggg
      841 tgatcttcag cgattgcctg catcagtacg atgccgcgga acgagaattg aaacgcgcgc
      901 tcgcgctgaa ccctgcctat gcccccgcgc tcgtcaacct cgccaacctc cacgaggatc
      961 tcgggcgcag ggatcaggcg gcggacgctt atgaacgaat cctggcgctc gctcccgacg
     1021 atccgcaggt cttggcccgt tacgccgggc tcaagtcgtt cacgagcgcg gacgacccca
     1081 tgattgcgcg tctgcgcgct gtccgcggcc gcccggggct ctccgctgcc ggccgcgcca
     1141 gcgtcgagtt cgctctcggc cgggcactcg atgcgtgcgg cgcgtatgac gcggcgttcg
     1201 aggcaatcag ggcggcgaat cggcacagcc gtgaaagcgc ggggctcgcc ttccgcgact
     1261 acgatcttaa ggcccaagag caattcgtcg accgcctgat cgaggccttt cccgcgggat
     1321 tgcgcgttgc agggccgcgc atgatgtcgc tgcggacggt gccgccgcag ccgattttcg
     1381 tgtgcgggat gttccgttcg ggctctacgc tcacggagca gctgctcgcg ggtcattccc
     1441 aagtcaaggc cggcggcgag ctcgacttcc tgccgcgtgc agtgcaaacc cagctcgcac
     1501 cctttcccga gtccatgcag acggtgtcgc aatttcggct cgaaacgctg gccgctgagt
     1561 atctgcagct gctgcgggat gtatttccgc aggcaacgta cgtcaccgac aagcgtcccg
     1621 ataattttct ctacatcggg ctcatcaaac tgctgtttcc gaacgcaaaa atcgtgcaca
     1681 cgacgcgcaa tgccttggac aactgcctat ctatttattt cttgcacttg gatcagggca
     1741 tgagctacgc cctcgatctc atggatatcg ggcacttcta cgggcagtac ttgcgcttga
     1801 tggcgcactg gaagacactc ttcggcgcgg atattctcga cttgagttac gattcgctgg
     1861 tgagcgagcc gcagcccgtc atgcggggat tgctgcaatc actcgacttg ccatgggatg
     1921 aacactgttt gtcggtgccg agcgccggca gggcgattcg cacggccagc gtttggcagg
     1981 tacgagagcc tttgtatcag cgctcctcgg gccgaagccg caactacgag cgccaccttc
     2041 aggtgctgcg ggagtatctc gccctccaga gccgctaatc ccaaggcatc gatgttgctg
     2101 atacgcaaca aaatcgtcga tttccggcgg cagggaatac gttttcgcag tctgctcgaa
     2161 ccgctgagag cgccgcggcg ctgtaaaaat tgcattgacc gcgcgtgtct gccaggcccg
     2221 caagccgccg ccgctgcaca tcgccgcctg agggtcgact accaataccc gttatttcca
     2281 gggtttaccg atgccggtaa tttgggttgc gatcataatt ttgggacacg atgaagctgc
     2341 ccgaacatcc gctcgaggtg ctcgcgcggc gcgtcggcca agtttttttt cgttgcgccg
     2401 aaggaatttt gcatccggaa gccgatttaa gacgatgagg cgccttggca acaccgaatt
     2461 tgcatggtgt aatcgcaaca tatctctgac tggccgtctg gggaaaagtt cgacacatgg
     2521 agccagtcag tccgtactga ccgtttttcg tgagggagca agacatgggg aacaatcgca
     2581 aattgcaagt ggcggtgcgc gcggcgctcg cactcgcgac tgcctccgct gccattccgg
     2641 cagccgaagc acagaccgcg acggctgcga acaacgaagc cacgaccggc gcgctcgaag
     2701 aggtcgtagt caccggctcg cgtattcaat cgacaaatct cgtttccatc agcccggtaa
     2761 ccaccgtcac cgccgaagcg attcagcaga cgggtttgac gcgcgttgag gacattctca
     2821 ataacttgcc gcaggtgttc gcaggccaag gcagcaacgt atcgaacggc tccgacggca
     2881 cggcaaccgt cgacttgcac gatctcggcc cgcaacgcac cttggtgctg gtcaatggcc
     2941 ggcgcttggg tcccggcggc ggggccaccg ccaatgcgtc ggacatcaac caaattccgg
     3001 cgcagctgat cgagaaagtc gaggtgctga ccgggggtgc atcggcgacc tacggcgcgg
     3061 acgccgtgtc cggcgtcgtc aacttcatcc tgaataccca tttcgagggt gtgaagatcg
     3121 atgccaacta cggcttgtat cagcacaaca atcacgatgg atttgccgcg ggcgtcgcgg
     3181 atgcacaagg cgatccggtg acgcagcccg gcagcgtcga cgccggcttc aacaaggact
     3241 tggccgtcct catgggttcg aatttcgccg acggcaaggg caacgcgacg gcctacgtca
     3301 cgtacaccaa tcaagcgccg gtactcgaag gccgttatga ctacagcgcg tgtactttgg
     3361 agggcaagcg gacgagcgcc acgacaacgg atgtgagttg cggtgggtcg tacaccagcc
     3421 gcggcggtgc cttcctcggt tacaacgcag cgtttagcaa gaacacggtg tatgacaccg
     3481 tcgatccgaa gaccggcatt tttcggccgt tcaatacggc gaccgatctt tttaattacg
     3541 gcccgatcaa ttactaccag cgcccgaacg aacgttggac ggccggctcg ttcatcaact
     3601 acgacgtgaa cgatcacgcc aatgtgtatt acgagttgat gtacatgaag aacaactcga
     3661 tcgcgcaaat cgcaccgggc ggcgacttcg gcaatccggc gaccatcaat tgcggaccgg
     3721 tgggggttgg gttgcccggt aacccgttgc tcacggcgca agaacgcagc gtcatttgta
     3781 cgccggcgaa catcgccgcg caggcgcccg gttcaccgcc gaacacggtg ctgctcaatt
     3841 acattctgcg gcgcaacgtc gaaggcggcg gccgccaagc ggcgtttcag aacgaatcct
     3901 accgcaacgt actcggcgtc aagggcgact tcgccgaggc gtggaaatac gatgcgtatg
     3961 ctcagttcaa ccatgtgacg acctatcaaa acaacttgaa ttatttttcg aacagcgcca
     4021 tcgataacgc gctggacgtg gtcgagggtc ctaccggacc aacgtgcgaa tccgtcatca
     4081 acggcaccga tcccgcctgc gtgccctaca acatctgggt gccgggcggc gtcaccaagg
     4141 cggcactggc ctacttgaac attccgaact cgatcacagg attcagctcg gagtatgcgg
     4201 cgcacgcgga cgtgagcggc gatctcggca agtatggtgt gcagataccg acggcgaaga
     4261 gcggcatgca ggtgaacttc ggcgccgagt ggcgcgaaga cacggtgcaa accgatccgg
     4321 attatgccac ccagcaggga ctcgcctcgg gcggagccgg tgcgataccg cccctgtcgg
     4381 gtcaaatcca cgtcgcggaa gtgttcaccg agttcaactt gcccatcatg gacagcatcc
     4441 cgggcgcgga tcagttggcc ctcgaaggcg gctatcgtta ttccagctac aacctgggct
     4501 tcaagacgaa tactttcaag atcggcctcg aatgggcgcc ggtgagcgac atccgtttcc
     4561 gcggcggctt caaccgcgcg gtccgcgctc ccaacatcgg cgagctgtat tcctcaccgt
     4621 cgatcggtcc ggacggaacg atcgatccgt gctggggcac gccggtgtac acggcggcgg
     4681 agtgcgcgcg aaccggtgtg acggccgccg aatacggcaa gatcggggtc aattccgcgt
     4741 accagatcaa cacgagctcg agtggcaacc cgaatttgac gccggaaaag gcgaatacca
     4801 catcgttcgg cttcgtgttc cagcctagct tcttcacggg cttcagcgca tcgcttgact
     4861 actacgacat caagatcacg aatgcgatcg tctcaagcac gggcgtggcg actgcgatta
     4921 tctcggagtg cgccctgacc ggttctgctc aggcatgtaa tctcattcat cgttctgcag
     4981 acggttcttt gtgggagagc tccgccggtt ttgtcgatac acccacctcc aatgtgggcg
     5041 tgatctcgac caaagatgtc gacctgaaga tgcactattc gacgggcatc ggcaatgcgg
     5101 gcaacttggc gttcaacttg gaaggcacct acgtgatcaa caacatcacg acgccgttcc
     5161 cgggtgcacc ctcgttcgat tgcgcgggac tctggggtcc gacctgcggc aaccccttgc
     5221 cgaaatggcg tcatatattc agcacggatt gggcgacccc ctggtcgggg ctcgatctga
     5281 atctgcggtg gcgttatttc ggcgcatcga atgttgaaac cagcagcggc agccccgact
     5341 tggccttcat cacgaccgat tacccgggct tcaaccggat ctcgaactac agctatatcg
     5401 acacgtcggc ggcgatggaa gtggcgaagg gtataacggt gcgcttaggc atcaacaacc
     5461 tgctcgacaa ggatcctccg accgtgctga gcgggaactg cgccgcgggt ccctgcaacg
     5521 gcaacacgtt ctcgcagagc tacgatgtac tcggacgcta tgtctacttg cacgtagcgg
     5581 cgaagttcta aacagcttcg ttttcgatgg ttgtcaacgg cgggcttcgg cccgccgttt
     5641 tttttccggt cgtgcgcagc gaccggcggc tgagagaatc gctaattgct cttcgggttg
     5701 aagacgttct tttgctgcac tcgagccgtc atgtcctgag actctttcgt ctggcgctcc
     5761 aaatcgtccg ccgtgccgca gctcttggtc tcaaagcgcg tgccgatttg cgcctcgcgc
     5821 cggcaccaca gcgtctggcc attgtgtgtt tcgggtttgt atcctgcagc tcgcaaagtc
     5881 ttgacctgcg tttccgtgct gccgctggtt gcagcgtcgg tcttggcggc ggtcggagcg
     5941 gccgccgggg tggctggtga agtgcttgca gacgagaccg cgggtgcggc cggagtcgca
     6001 gcagcagtcg cgttttgagc cggcggcgat ggcgggtcgg ccgccagcgc ctgcgaagcg
     6061 acgccgccga tcgacagtaa aatcagcaag ctattgattg aacgcatgat attcctttgg
     6121 ctacgtaaca gggggtgtcg cacggttatg ccacaaagat tccaggccgg tcaaacacat
     6181 ggaatgctta tgtctcacta atgcatgcgg tgcgcgtgat gcgtgcgtcg cgtgggggtg
     6241 gtcagcagga atgagccgag cgcaccccgc tggatcgtca cggtgttgcc ggccgcgagc
     6301 agaggatctt cgctgtcgag ctgccaagat tgtccattgt cgagctcgag cagcatgtgt
     6361 ccgctccccg agtgccatac gctggtgact ttcgcgctga tcgattcgat ctgcttttgc
     6421 ggattcactt tctgcagttg tgctgcactt aaaccgaagt cttccttctt cggaggcaca
     6481 gcggcggctg gcggaactgc ggcggctggc ggggctgccg ccgctgttgg ggccggcgcc
     6541 ccggcaggcg cattcgcagt tgcgccgtga ttggcggcca gggcgtcgta gcaggcgagc
     6601 cgctcgtcgc gagagctgat cgccgcgcag cgcgcaaggt ctttgccgat gagcgccgct
     6661 gcgtcgtcgg cgaagcccgg ggtagcggaa cctaaaccca aaatcaaggc agccaagatc
     6721 gagcgcgagg tgcatcgcct catcggaagc tctcggccgg catctgatag taacttacga
     6781 cgacttcggt cgccgcgcgc accatgtcgc gctcggcctc gctcaagaag ggattccaga
     6841 tgtcgaatat caatatggcg cgcggcatgt ccgagccgtt ccaggcttcg tggtcgatgg
     6901 tgtcgtcgaa tacccaggcc tgcccggccc gcacttctcg cgtcgtgccg ccgactcgaa
     6961 accggcaatc gggcgggacg atgagcggca ggtgcaccgt gagacgcgtg ttggtgacgc
     7021 cggtgtgcgc cggaatggta gtcgaggcgt ccagaatgga gaagaacgcg gtcggtgcgc
     7081 gacccgcgac ttcgcagcgc ggcgcgtgcg cgagcgccgc cgcggtgcgc gggcaacgcg
     7141 caatgtgcgc gggctgcgcc gtgccctgat tccaaagaaa gtacgcgctc caacgccgcg
     7201 agcggttgag ctctttccat tgatcgatgg gcaatcccgc cggataatcg atatacggct
     7261 gcagacccgc gcgatcggcg atcaacacgt tcgtcacctc ggaccgaatg tcctcggtcg
     7321 ccgcctcgat ggcgtcgagc caggggaaat ccgcccgctc gaaaaactcg atcgccggta
     7381 tctccggaaa gtacatgaag gtgggg
//
LOCUS       DASWZT010000193         5718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_6198, whole genome shotgun sequence.
ACCESSION   DASWZT010000193 DASWZT010000000
VERSION     DASWZT010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6198"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VGE92_05135"
     CDS             complement(<1..365)
                     /locus_tag="VGE92_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY1313236.1"
                     /translation="MIRVLLVDDHAVVRMGFRLLLQSVADMSVVAEADSGEAACRLYL
                     EHTPDVVVMDLAMPGMGGLEALRRIRARHPQAQVLTLSAQDDPMHARRALQEGALGFL
                     SKRSAPEALLEAVAVVAAGR"
     gene            complement(362..1714)
                     /locus_tag="VGE92_05140"
     CDS             complement(362..1714)
                     /locus_tag="VGE92_05140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1313237.1"
                     /translation="MRLRTRLNLVVAALSAAFLLVLIASEVQSMRTSVREEIEAANRV
                     ASQLLGRLGAVYSQVGGPELVLRFLEQLGRVRANEVYLQAPTGEILYQSPRATYKAGR
                     EAPLWFAHLLAPQPERYTYPLRGGVLLVVQSQPSRAVLDAWDDITRLSAIAVAMFVVV
                     NALAFWSVQRTLKPFPVIADGLERIQRGDLHFRLPDLTGTEAHAIGAAFNRMAEAVQD
                     KVLAERKAQDAEARLDERREMASLADQRVEEERRLIAHELHDEFGQSVTAIRSLALAI
                     AAQLGGRDPAIGDVARLISEEAARLYDAMHGLIPRLTPLSLDTLGLAATLESLVRDWQ
                     RRHPAIALSLNQELSIELGPSITLTIYRVVQEGLINALRHAQASRVDIEVICDAERIV
                     ATVTDDGVGLASEWARPGHFGLRGLTDRVQQLGGTFTIGNREGRGVRLVADIPLGVPA
                     "
     gene            2005..2919
                     /gene="pqqB"
                     /locus_tag="VGE92_05145"
     CDS             2005..2919
                     /gene="pqqB"
                     /locus_tag="VGE92_05145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HEY1313238.1"
                     /translation="MRIRVLGSAAGGGFPQWNCNCPNCSSQRAGTLRGRARTQSSIAV
                     GGGTSWVLVNASPDILAQLHANPDLQPGRAMRDTALGAIILVDGQVDHTTGLYMLRES
                     ARPWPIWCTDSTYADLTRGNPVFGVLGYFCGVDRRRIDLDGGGFSVDAVPGVRWRALP
                     VASKPAPYSPNRDSPVAGDNLALVIEDEASGKTVVYAPGLSAIDDAVWTAMQSAACVM
                     VDGTFWTDDEMIRLGISAKRARDIGHLPQSGAGGMLEWLDRLPAATRRILIHINNTNP
                     ILNEASAESAEVARRGVEVAWDGMEVVL"
     gene            2916..3611
                     /gene="pqqC"
                     /locus_tag="VGE92_05150"
     CDS             2916..3611
                     /gene="pqqC"
                     /locus_tag="VGE92_05150"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR02111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HEY1313239.1"
                     /translation="MSAAWSREEFEAQLRTQGRAYHIHHPFNVLLNSGGATPAQIRGW
                     VANRFYYQINIPIKDAAILSNCTDRAVRRSWVQRILDHDGYGDDAGGIESWLRLAEAV
                     GLSRDTVESLTEVVPGVRFAVDAYVNFARRAPWPEAVCSSLTELFAPEIHKQRLATWP
                     QHYPWIDLAGLQYFQSRVSLARRDVEFGLAVTLERFNTRELQERALDILKFKLDVLWQ
                     MSDAMALRYKVPA"
     gene            3608..3892
                     /gene="pqqD"
                     /locus_tag="VGE92_05155"
     CDS             3608..3892
                     /gene="pqqD"
                     /locus_tag="VGE92_05155"
                     /inference="COORDINATES: protein motif:HMM:NF002535.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HEY1313240.1"
                     /translation="MTAPVVDVASRPAMERGFRLQWEAAQNAHVLLYPEGMVKLNTSA
                     GEIMKRCDGAATIADITSDLERAFATSNLSADVIRFVTMAVDKRWLRILP"
     gene            3871..5046
                     /gene="pqqE"
                     /locus_tag="VGE92_05160"
     CDS             3871..5046
                     /gene="pqqE"
                     /locus_tag="VGE92_05160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HEY1313241.1"
                     /translation="MAANSPVVDPAGTVSAPRPGPPLWLLAELTYRCPLHCAFCYNPT
                     DFARTSAELSTEDWLRVLREARALGAVQLGLSGGEPLSRDDLEPIVSEAHALGFYTNL
                     ITSGVGLTDARLRTLKKAGLDHIQLSFQDSTRQMNDFLSSTRTFDLKSAVAALIKKYD
                     YPMVLNVVLHRLNIDHVEEILEMALRLGAEYVELANTQYYGWAFHNREQLLPSREQVE
                     RAEAVMQRFRARVERQMKIYFVVPDYFERRPKPCMNGMGSIFLTITPDGTALPCHAAR
                     MLPGLEFPNVRSASIREIWYESAGFNQFRGDAWMKEPCRSCPEKARDFGGCRCQAYLL
                     TGDAANADPVCDLSPHHHLITEAVARANRPLAASRLAPEEKPLVFRSERNSTTLFVK"
     gene            5063..>5718
                     /locus_tag="VGE92_05165"
     CDS             5063..>5718
                     /locus_tag="VGE92_05165"
                     /inference="COORDINATES: protein motif:HMM:NF027806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313242.1"
                     /translation="MQWITWLFVAAVVAETLTRLWLGTRQIAAVQAHRNQVPELFREQ
                     IALADQQKAADYTTARVRLGRGATLVEALVKLMLTLGGGLAAVDAVWRHTSLGEPWRG
                     ALLVASVTLLLQLVGLPFSLWRTFRIEARFGFNRVSPRLYAVDLAKQWLLSVLLGGPL
                     LLATLVLMDKAGPWWWLWVWAIWLGWTLLLTWAAPKFIAPFFNRFSPLTDEALKRRVE
                     A"
BASE COUNT         1036 a         1777 c         1840 g         1065 t
ORIGIN      
        1 cggcccgcgg caaccaccgc caccgcttcg agcagcgctt cgggagcgct gcgtttcgaa
       61 agaaatccga gcgcgccttc ctgcaacgcg cgccgcgcgt gcatcgggtc gtcttgcgcc
      121 gacagcgtca acacttgcgc ctgcgggtga cgcgcgcgaa tgcggcgcag cgcctcgaga
      181 cctcccattc ccggcatggc cagatccatc acgacaacgt cgggagtgtg ctcgagatac
      241 agccggcacg cggcttcgcc ggaatcggct tctgcgacca ccgacatatc cgccacggat
      301 tgcaacagca gtcgaaatcc catacgcacc accgcgtgat cgtcgaccag cagcacgcga
      361 atcatgcggg aacacccaag ggaatgtcgg cgacgagcct gacaccgcga ccctcgcgat
      421 tgccgatagt gaaggtgccg cccaattgct gcacgcggtc ggtcaaaccc cgcaaaccaa
      481 aatgcccggg tcgcgcccat tcgctcgcca aaccgacacc gtcatcggta accgtggcga
      541 cgatccgctc ggcgtcgcaa atcacctcga tgtcgacgcg cgaggcctgc gcgtgacgca
      601 gagcgttgat cagaccctcc tgcaccacgc gatagatcgt cagagtgatg ctcggcccga
      661 gttcgatact cagctcctgg ttcagcgaaa gggcaatcgc gggatgacgc cgttgccagt
      721 cgcgaaccag actctcgagc gtcgccgcga gcccgagcgt atccaaggac agcggcgtca
      781 ggcgcggaat caagccatgc atggcgtcgt agagacgtgc ggcttcctcc gaaatcaatc
      841 gcgccacatc gccgatcgcg gggtcccgtc cgccgagttg cgctgcaata gcgagcgcca
      901 ggctgcgaat cgcggttact gattgaccga actcgtcgtg cagttcgtgg gcgatcaatc
      961 gccgctcttc ctcgacgcgt tgatccgcca gggatgccat ttcgcggcgc tcgtcgaggc
     1021 gtgcttcggc atcttgcgcc ttgcgttcgg ccagaacctt gtcctgcacc gcttcagcca
     1081 tgcggttgaa tgctgcgccg atcgcgtgtg cttcggtgcc cgtgagatct ggcagccgga
     1141 agtgaaggtc accgcgttga atgcgctcga gcccgtcggc aatgaccgga aagggcttca
     1201 gcgtgcgttg caccgaccag aacgccagcg cattcaccac gacgaacatc gcgactgcga
     1261 tggctgacaa ccgggtgatg tcgtcccatg cgtcgagcac ggcgcgcgag ggctgagatt
     1321 gcacgaccaa caacaccccg ccgcgcagcg ggtacgtata ccgttccggt tgcggtgcca
     1381 ggagatgagc aaaccacagc ggcgcctccc gaccggcctt gtaggttgcc cgcggcgatt
     1441 ggtacaggat ctcccccgta ggcgcctgca aatacacctc attggcgcgg acgcggccga
     1501 gctgttccag gaaccgaagc acgagttccg ggcccccgac ctgggaatag acggccccga
     1561 gccgcccgag gagctgcgat gccacccgat tcgcggcttc gatctcctcc cgcaccgacg
     1621 tgcgcatgct ttgaacctcg gaggcgatca gcacgagcag gaacgcggcg cttaaggccg
     1681 caaccacgag attcaggcga gtccgcagcc gcatcagccg catggtacca gggccgtgtc
     1741 tgggacgggc cgcgctgccg gtctttggga cataacgtgc tcggcctcat gaaaatcatg
     1801 agtccacatt catgatgccg ctgatgggac ggtccggcca ttgagagcac catggcggcg
     1861 taatcgatca aggagcattg aaatgatttg ggaaacccca caagcggttg aattgcgctt
     1921 cggaatggaa atcacgatgt atgtgtcgaa tcgctagata gcgagggtgc gcgcccgcat
     1981 ctgcgggcgg gcgcctattt cttagtgaga atccgggtac tggggtcagc cgccgggggc
     2041 gggtttcccc agtggaattg caactgccca aactgcagca gtcaacgtgc gggcaccttg
     2101 cggggtcgcg cgcgaaccca gtcgtccatc gccgtcggcg gcggcacttc gtgggtcctc
     2161 gtcaacgcct cgcccgacat cttggcgcaa ttacacgcaa atcctgatct gcagccaggc
     2221 cgcgccatgc gcgataccgc gctcggcgcc attattttgg tcgacgggca ggtagaccat
     2281 acgacggggc tctacatgtt gcgcgagtcg gcccgtccct ggcctatctg gtgtacggac
     2341 agcacgtacg ccgatcttac gcgcggcaat cccgtgttcg gtgtgctcgg ctatttctgc
     2401 ggcgtggacc ggcgccgcat cgatctggac ggtggcggat ttagcgtcga cgcggtgcct
     2461 ggagtgcgct ggcgggcatt gccggtggcc agcaaaccgg ctccctattc tcccaaccgg
     2521 gattcgcccg tcgccggcga caacctggcc ttggtcatcg aggacgaggc cagcggcaaa
     2581 accgtggttt acgcgcccgg cttgagtgcc attgacgacg cggtctggac cgcgatgcaa
     2641 tcggccgcct gtgtgatggt cgacggcaca ttctggaccg acgatgagat gattcgattg
     2701 gggatttccg cgaagcgcgc gcgcgacatc gggcacctcc cgcaaagcgg agcgggcgga
     2761 atgctggaat ggctcgatcg cctgcccgcc gccacgcggc gaatcttgat tcacatcaat
     2821 aataccaatc cgattctgaa cgaggcgtcc gcggaatcag ccgaggtggc gcgccgcggc
     2881 gtcgaggtcg cgtgggatgg catggaggtg gtgctatgag cgcagcctgg agccgtgagg
     2941 aattcgaggc gcagctgcga acccaaggcc gcgcctacca cattcatcat ccgttcaatg
     3001 tgctcttgaa ctcgggtggg gcgacacctg cgcagatccg cggctgggtc gccaaccgtt
     3061 tctattatca aatcaacatc ccgatcaagg atgcggcgat tttgtccaac tgcaccgatc
     3121 gcgcggtgcg ccgctcgtgg gtgcagcgca tcctcgatca tgacggttac ggcgacgatg
     3181 ccggtggaat cgagtcttgg ctgcggctgg cggaggcggt gggcttgtcg cgcgacacgg
     3241 tcgagagtct gaccgaagtc gtgccggggg ttcgattcgc agtcgatgcg tacgtgaact
     3301 tcgcacgccg cgcaccgtgg ccggaggcgg tctgctcgtc gctcaccgaa ctgttcgcgc
     3361 cggaaattca caagcaacgg ctggcgactt ggccgcagca ctatccctgg atcgacctcg
     3421 ccggattgca gtattttcaa agccgggtga gcctcgcgcg ccgcgacgtg gaattcggac
     3481 tcgcggtgac cttggagcgc ttcaacacgc gcgaactaca ggaacgcgcg ctcgacatct
     3541 tgaaattcaa actggacgtg ctgtggcaga tgagcgatgc catggcattg cgatacaagg
     3601 tgcctgcgtg acggcgccgg tcgtcgatgt cgcctcgcgg cccgccatgg agcgcggctt
     3661 caggctgcaa tgggaggcgg cgcaaaatgc ccacgtgctg ctctatcctg aaggcatggt
     3721 caagctcaac acgagcgccg gtgagatcat gaagcgctgc gacggtgcgg ccacgatcgc
     3781 cgatattacg tccgatttgg aacgcgcctt cgcgacctcc aatctgtccg cggatgtcat
     3841 tcgattcgtt accatggcgg tggacaagag atggctgcga attctcccgt agtcgacccc
     3901 gcgggcacgg tcagcgcgcc ccggccgggg ccgcccctgt ggctgctcgc ggagctcacg
     3961 taccgctgtc ctttgcactg tgctttctgc tacaacccga ccgacttcgc gcgcacctcg
     4021 gcggagctca gcaccgagga ctggctgcgg gtgctacgcg aggcgcgcgc actcggcgcc
     4081 gtgcagctgg ggctatcggg cggcgagccc ttgtcgcgcg acgatctgga gccgatcgtg
     4141 agcgaggcac atgctctcgg cttctatacg aatctgatca cctccggtgt cggtttgacc
     4201 gatgcgcgcc tgcggacgtt gaaaaaagcc ggcttggacc acatccagct gtccttccag
     4261 gacagcaccc ggcaaatgaa tgattttctg agcagcacgc gcacgttcga tttgaaatcg
     4321 gcggtcgccg cactgatcaa gaaatacgac tatcccatgg tgctgaatgt ggtgctgcac
     4381 cggctgaaca tcgaccatgt cgaggaaatt ctcgaaatgg cgttgcgcct gggcgccgag
     4441 tatgtggagt tggcgaacac tcagtactat gggtgggcgt tccacaaccg cgagcaactg
     4501 ctgcccagcc gcgaacaggt cgaacgggcg gaagcggtga tgcagcgctt ccgcgcgcgc
     4561 gtcgagcggc agatgaagat ttatttcgtg gtgcccgatt atttcgagcg tcgcccgaag
     4621 ccctgcatga acggcatggg ttcgatcttt ctcaccatca cgccggatgg aaccgcgctt
     4681 ccctgccacg ccgcccgcat gttgccggga cttgagtttc cgaatgtgcg cagcgcgagc
     4741 atccgggaaa tctggtacga gtcggcgggc ttcaatcaat ttcgcggtga tgcttggatg
     4801 aaggaaccgt gccgcagctg cccggaaaaa gcccgtgatt ttggcggatg ccgctgtcag
     4861 gcgtacttgc tcacgggcga cgccgcgaac gcggatccgg tgtgcgatct gtctccgcac
     4921 catcatttga tcaccgaggc cgtcgcccgc gcgaaccggc ctttggccgc atcgcggctt
     4981 gcgccggagg aaaaaccgct ggtgtttcgc agcgagcgca attcgactac gctgttcgtc
     5041 aagtaaacgg aatcttgaat cgatgcagtg gattacatgg cttttcgttg ccgcggtcgt
     5101 ggccgagacc ctgacgcgac tgtggctggg aacgcggcag attgccgcgg tgcaggcgca
     5161 tcgcaaccag gttccggagt tgtttcgcga gcagatcgcg ttggcggatc agcaaaaggc
     5221 agctgactac accaccgccc gcgtgcggct cgggcgaggc gcgacgctcg tcgaagcgct
     5281 cgtgaaactg atgctcaccc ttggcggcgg gctggcggcg gtcgacgccg tgtggcgcca
     5341 tacgtcactc ggcgagccat ggcgcggcgc cttgctggtc gccagcgtaa cgctgctgct
     5401 gcaactcgtc gggctgccgt tttcgctgtg gcgaacgttc agaatagagg cccgctttgg
     5461 attcaatcgg gtgtcgccgc ggttgtacgc cgtggatttg gcgaaacagt ggctgttgtc
     5521 ggtactgctc ggcggcccgc tgttgttggc gacgctggta ctcatggaca aggccggacc
     5581 gtggtggtgg ctgtgggtgt gggcgatctg gctcggctgg acgttgctgt tgacgtgggc
     5641 cgcgcccaag ttcatcgcgc cattcttcaa tcgcttctcg ccgctcaccg acgaggcact
     5701 gaagaggcgg gttgaggc
//
LOCUS       DASWZT010000194         2411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_25668, whole genome shotgun sequence.
ACCESSION   DASWZT010000194 DASWZT010000000
VERSION     DASWZT010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25668"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VGE92_05170"
     CDS             <1..456
                     /locus_tag="VGE92_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313243.1"
                     /translation="FKDIAADAQYQFIGEDHLATFLGTYISEHQTLNASVLDGFAANT
                     DNHLKTLKLTAEYYYQRRIGGTIGYFSTTGGSDSLLYAPAPVTGSATNSPDTTGYVLE
                     VNYLPWLNTKLQAQYTGYQKFNGGGTNYDGSGRNASNNNTMYLLVWLNF"
     gene            478..1116
                     /locus_tag="VGE92_05175"
     CDS             478..1116
                     /locus_tag="VGE92_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY1313244.1"
                     /translation="MIKPLHIVALAMSLFECGGSIAYGADVAPDNTSDAQKSALRKTV
                     ETCGTCHGVNGRSVSPTFPNLAAQQAAYVELQLKAFKDQSRGDPDAQAYMWGMASQLS
                     DATISALAGYFAAQPAAPGRGGSSALIAQGKHLFEEGVPARGIPPCASCHGAHAEGMA
                     AFPRLAGQHAPYLFKQLLVIQSVLRTAPVMHGVIKDLTKDQMQAVVAYLESI"
     gene            1397..2242
                     /locus_tag="VGE92_05180"
     CDS             1397..2242
                     /locus_tag="VGE92_05180"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEY1313245.1"
                     /translation="MRQSIQNLQGLLDTALNGLPPAITPDAVHRTRTAARRLRALLCA
                     FKRSLQRSAFRHYAAALQELAHDLDTLREADVTQHILANLSRDYRGLSRVKLDGLKSE
                     FVQSRTRAAGDLQSAMATKRWPARLVKLRDAASDRALVVATHESMRSMTQRVLTRRRR
                     RLRAALRYRGQGPRRLHKIRLKVKTLRYLLEQCESSGCDAVRAEVKQLRELQDCLGEL
                     HDAWCLGRALRRYRRHHRRATTELSTDIETRRAELFHRFRKHRKALRRLWRAARNATV
                     TRQDT"
     gene            complement(2223..>2411)
                     /locus_tag="VGE92_05185"
     CDS             complement(2223..>2411)
                     /locus_tag="VGE92_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313246.1"
                     /translation="DYNRFAVGAFFFSVAAFDFAYCADWLWDSDDVVIYDDPDHVGWY
                     LGYNVRLGTYVHVTYLGE"
BASE COUNT          524 a          725 c          738 g          424 t
ORIGIN      
        1 ttcaaggata tcgccgccga tgcgcagtac cagttcatcg gcgaggacca tctggccacg
       61 ttcctgggaa cttacatcag cgagcaccaa acactcaacg ccagcgtttt ggacggattc
      121 gccgcaaata cggacaacca cctcaagaca ctcaaattga cggctgagta ttattaccag
      181 cggcggattg gcggcaccat cggctacttc tcgaccacgg ggggcagcga ttcactcctc
      241 tatgcgccgg cgccggtcac gggcagtgcg acgaatagtc ccgacaccac cggctacgta
      301 ctcgaggtga actacctgcc gtggctcaac acgaaactgc aggcgcaata caccggctat
      361 cagaaattca acggcggcgg aaccaactat gacggctccg ggcgtaacgc gagcaacaac
      421 aacaccatgt acttattggt gtggctaaat ttctaattcg gacttaggga gccagagatg
      481 atcaagccac tccacattgt cgcccttgca atgagcctat tcgagtgcgg cggcagcatt
      541 gcctacggcg cagatgtcgc gcccgacaat acgagcgatg cccagaagtc ggctctgcgc
      601 aagacggtgg aaacctgcgg cacctgccac ggagtcaatg gccgcagcgt gtcgccgacc
      661 tttccaaatc tggcggcgca gcaggccgcc tatgtcgaat tgcagctgaa ggcgttcaag
      721 gaccaatcgc gcggggatcc ggacgcccag gcatacatgt gggggatggc gtcccaactg
      781 agcgatgcga cgatcagtgc gttggccggc tattttgccg cacagcccgc tgcgcccgga
      841 cggggaggga gttccgctct gatcgcgcag ggtaagcacc tgttcgagga gggggtaccc
      901 gcccgcggga tcccgccgtg cgcctcatgt cacggcgcgc atgccgaggg tatggccgca
      961 ttcccacggc ttgccgggca acacgcgccc tatttgttca agcagctgct cgtgattcag
     1021 agcgtgctgc gcacggcgcc ggtcatgcac ggcgtaatca aagatctcac gaaggatcaa
     1081 atgcaagccg tggtggccta tctcgagtcg atatgatcgc cgggcaggtg cgtcaaaaga
     1141 cggattcgcg caacggaccg tggccatagc cgccgaccac caacaagtca acgcttgagc
     1201 tgcggcggcg gcgcaccggg cgcttcgcgt actatacgca tatattccga tgctatttcg
     1261 gcgcgtcccc gagattcgaa aaccgtcgcg gaaaggacga gagatgcgaa atcaaaagcc
     1321 cgctcgcgtt gaacatatgc ctcgagggca accgcaagcg cggggccatg cgcctgaatc
     1381 aacggtcacc gcggcgttgc gtcagtccat ccagaacctg cagggattgc tcgatacggc
     1441 actaaatgga ctgccgccgg cgatcacgcc ggatgcggtg catagaacgc gcactgccgc
     1501 acgtcgattg cgagcgctgc tgtgcgcctt caagcggtca ttgcagcgca gcgcattccg
     1561 tcactacgcg gccgcgttgc aggaattggc tcatgatctc gatacgttgc gggaggcgga
     1621 cgtcacgcaa cacattctcg cgaacctgtc tcgagattac cgcggactca gccgggtaaa
     1681 actggatgga ttgaagtccg agtttgtgca aagtcgaact cgcgcagctg gcgacctgca
     1741 atccgcgatg gcgaccaagc ggtggcccgc gcgtctggtg aagctgcgag acgccgcatc
     1801 cgaccgggca ttggtcgttg caacacatga gtcgatgcgt tcaatgacgc agcgtgtctt
     1861 gacgcgtcgc cgccggcgcc tgcgtgcggc acttcgttac cgtgggcagg gcccgcggcg
     1921 gttgcacaag attcgactca aggtcaagac gctgaggtac ttgttggagc agtgcgaatc
     1981 gagcggttgc gatgcagtgc gcgccgaggt caagcaatta cgcgagctgc aagactgcct
     2041 gggagaactt cacgacgcat ggtgcttggg acgcgcgctc aggcgctacc ggcggcacca
     2101 tcgtcgtgcg acgactgaat tgtcgacgga catcgagact cgccgagcgg aactctttca
     2161 ccgcttccgc aagcaccgca aggcgttgcg gcgactatgg cgcgctgcga ggaatgcgac
     2221 ggtcactcgc caagatacgt gacgtgcaca taggtgccga gtcgcacgtt gtagcccaga
     2281 taccaaccga catggtcggg atcgtcgtag atcacaacgt cgtcgctatc ccatagccag
     2341 tcggcgcagt aggcgaaatc gaaggcggcc accgaaaaga agaaggcacc gaccgcaaat
     2401 cgattgtagt c
//
LOCUS       DASWZT010000195         3592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_88471, whole genome shotgun sequence.
ACCESSION   DASWZT010000195 DASWZT010000000
VERSION     DASWZT010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88471"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1469)
                     /gene="mutS"
                     /locus_tag="VGE92_05190"
     CDS             complement(<1..1469)
                     /gene="mutS"
                     /locus_tag="VGE92_05190"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEY1313247.1"
                     /translation="MPEPSTPLMRQYAAVKKEHPTALLFFRLGDFYELFFDDAIVAAK
                     ELQITLTSRNKEKGIAVPMCGVPHHAAEGYIGKLIRKGFKVAICEQMEEARLAKKHLG
                     TKLVRREVTRVVTPGTAADALLGSEENNFLAALAQVGDRVGFAALDLSTGEFRATEFS
                     GDSALQRIQEELEQLRPKEVLYGSGAPLFDANIGRSQANLPRPSGAACTETPLDDWIF
                     SPDHSIPLLENHFGVLSLEGFGLAGRTAAAAAAGAILYYVRTTQRGSLDHVDRIGWYE
                     RQNCLVLDAVTVRNLEMIEPLFAGADAGVTLFRSIDATITPMGKRLLRAWLLRPSLDL
                     AEINARLDAVDAGVSETIAREELRRALDGVLDLERLLSRVTLETANPRDVLALAASLA
                     KIPAIKNAMRALAAVRLTTLLGTLAELRDLRERIQTTITPEPPLSFADGGVIAAGVDR
                     DLDDLRELSRNSKQVLAQIEQRERGRTGIASLKVKFNSI"
     gene            1613..2599
                     /locus_tag="VGE92_05195"
     CDS             1613..2599
                     /locus_tag="VGE92_05195"
                     /inference="COORDINATES: protein motif:HMM:NF027609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4931 domain-containing protein"
                     /protein_id="HEY1313248.1"
                     /translation="MELRKDPITRSWVITGDDPAESPRTEGACRFCPDSADSPQVISS
                     MPSSGAWSARSVVHPTPLYHIEGEPGRRGDGIYDCMSSVGAHEVLVENPRHDRHLWNA
                     SDDEIGQFLRLAAERILDLKHDPRIKYVSFFKDYGPNAGQEFSHPTSQITATMFVPRR
                     VLYELRASREYFASKERCVFCDILNQEERQASRVIEARGDYVAVGPYAPRIPYETWIL
                     PRKHEASFERTGLSRPGLRVNLAALLRRTLQRVRALTEGFHLVLHTSPNSLHPSKTLG
                     YWKTIDDDYHWHIEILPVLAAKARSYTFKEVYYSPVSSETAVKQLREAKIDG"
     gene            complement(3044..>3592)
                     /locus_tag="VGE92_05200"
     CDS             complement(3044..>3592)
                     /locus_tag="VGE92_05200"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY1313249.1"
                     /translation="AHHVAITSTLAVIETFGSNRPPMERESAALKTLSPQAAKEYLLG
                     RSQMAERDLGGPMLKKEMNFEREFEAAGGLLMAGCDPSGYGGVVPGFGDQRNIELLVE
                     AGFSPVEAIRIAALNGAIYMGREASIGSVAPGKAADLVVLGGNPAEKIENVEKVELVF
                     KDGVGFDSAKLIQSVPELVGLR"
BASE COUNT          693 a         1189 c         1020 g          690 t
ORIGIN      
        1 aagatggagt tgaatttgac cttgagcgat gcaatcccgg ttcgcccgcg ttcgcgctgc
       61 tcgatctgcg ccagcacctg cttgctgttg cgcgagagct cgcgcaagtc atcaagatcg
      121 cgatcgactc cggcagcaat cacgccgcca tcggcaaagc tcagcggcgg ttcgggagtg
      181 atcgtggtct ggatgcgctc gcgcaagtcg cgtaattcag cgagcgtccc gagcagagtc
      241 gtcagacgca cggccgctaa agcccgcatc gcattcttga tcgcaggaat tttcgccagt
      301 gacgccgcca gggccagcac gtctctagga ttcgcagtct caagcgtgac ccggcttaaa
      361 agccgctcca ggtcgagcac gccatcgagg gcgcgacgca gttcctcgcg cgcgatggtc
      421 tcgctcacgc ccgcgtccac ggcatccagg cgcgcgttaa tctcggcgag atcgagcgag
      481 ggccgcagca accatgcccg cagcagccgc ttgcccatgg gcgtaatcgt ggcatcgatg
      541 gagcggaaca gagtgacccc cgcatccgcg cccgcgaata ggggctcaat catctccaga
      601 tttctgaccg tgaccgcatc cagcactagg cagttctgcc gctcgtacca gccgatgcgg
      661 tcgacgtggt cgagcgaccc gcgctgcgtc gttcgcacat aataaaggat cgctcccgcg
      721 gctgcggctg cggcggttcg tcccgccaaa ccgaatccct cgagcgacaa cactccgaaa
      781 tgattttcca gcagcggaat ggaatggtcg ggcgagaaga tccaatcgtc gagcggagtt
      841 tcggtgcacg ccgcgccgga cggtcgcggc aagtttgctt gagaacggcc gatgtttgcg
      901 tcgaacaagg gcgcccctga gccatacaac acttccttcg gccgcagttg ttccagctct
      961 tcttgtattc tctgcagcgc gctatcgccg gagaattccg tggcccgaaa ttcgccggtg
     1021 gaaagatcga gcgccgcaaa cccgacgcga tctccgacct gcgccagggc tgcgagaaaa
     1081 ttattttcct ccgagcccag cagcgcatcc gcagcagtgc cgggagtgac cacccgcgtt
     1141 acttcgcgcc tcaccaactt cgtgcccagg tgtttcttcg cgagccgcgc ctcctccatc
     1201 tgctcgcaga tggcgacctt gaaacccttg cggatcagct tcccgatata gccttccgcc
     1261 gcatgatgag gcacgccgca catgggcacg gcgatgcctt tctccttgtt gcgcgaagtc
     1321 agggtaatct gcaactcttt tgcggccaca atggcgtcat cgaaaaacag ctcgtagaaa
     1381 tcccccaggc gaaaaaaaag cagcgcggtc ggatgttctt tcttgacggc ggcatattgc
     1441 cgcatcagcg gagtggacgg ctcaggcatc gatatcgcga gttcgatctc ggcagcaata
     1501 aggggcttgc ggcggattat aacaaagccc atcctcgcct ttcgtacgca tttttgatgg
     1561 ggagggcgtt atgccagttg acgcgcgttc tcctgcagtg ctttcctagg gcatggagct
     1621 ccgcaaagat cccatcacgc gctcgtgggt catcaccggc gacgatcctg ccgaaagccc
     1681 acggaccgag ggcgcctgcc gtttttgccc ggattccgcg gactcgccgc aagtgatttc
     1741 ctccatgccc agcagcggag cgtggtcggc gcgctcggtt gtgcacccca cccctctcta
     1801 tcatattgag ggcgagcccg gccgccgcgg cgacggcatt tacgactgca tgagttccgt
     1861 cggcgcgcac gaagtcctgg tcgagaaccc ccggcacgac cgccatttgt ggaatgccag
     1921 cgacgacgag atcggccagt tcctgcgcct ggccgcggaa cgcattctcg atctcaagca
     1981 cgacccgcgc atcaaatacg tcagcttctt caaggactac ggtcccaatg ccggccagga
     2041 attcagccac cccacttccc aaattacggc cacgatgttt gtgcccaggc gcgtgcttta
     2101 cgaactgcgc gccagccgcg aatatttcgc ctccaaagag cgctgcgtat tctgcgacat
     2161 cctcaaccag gaggagcgcc aagcctcacg cgtcattgaa gctcgcggcg actacgtggc
     2221 cgtcggacct tacgccccgc gcatccctta cgagacctgg attctaccgc gcaaacacga
     2281 agcctcattc gagcgcaccg ggctatccag gcccggcctg cgagtgaatc tcgccgcgtt
     2341 gctacgccgc actctgcagc gcgtgcgggc gctcaccgag ggtttccatc tggtgctgca
     2401 cacctcgccc aacagcctgc atccttcgaa gactctgggc tattggaaaa ccatcgacga
     2461 cgattaccac tggcacatcg agattctgcc tgtgctcgcg gcgaaagctc gctcttacac
     2521 gtttaaggag gtctactact ccccggtcag ctccgagacg gcggtgaaac aactgcgcga
     2581 ggccaaaatc gatggctgaa cccgcgaaag ttgagcccag cgccgagatc gggctacggg
     2641 gctcattgcc atcgcctccg tcgccgatga gcagcgctcc atcgacttct tccagcggtt
     2701 gggcatgaga ctgcgcaaca gtttaagagt tgccggcgag ctgcgttggg cgcacatcga
     2761 atgcgagcaa gcgcatcgga tgctttcgcg cgccgaaaat ccggttgctg cccgccagcg
     2821 atccgtacgc ttcgaccgca acgcgccgaa tctcatcgcc ttgcggcaac ccttgatcgc
     2881 ggcggcaggg agcgtatcgc cgggtactta ccctcaatac atgcccaagg gcgaagttag
     2941 gttggaagat cccaatggtt actgtttgcg gatcggccag gcggattgat agagttcgcg
     3001 tgaaaatgca ggtctccttt gcgttcaccg cgcctgcgcg tgctcaccgc aagcccacca
     3061 actcggggac ggattgaatc agctttgcgg agtcgaaacc cacgccgtct ttgaacacca
     3121 gttcaacctt ctccacgttt tcaatcttct ccgccggatt gccgcccaac actaccaaat
     3181 cggcggcctt acccggcgcg accgatccga tgctcgcctc tctgcccatg tagattgccc
     3241 cattcagcgc ggcaatgcgg atcgcctcga ccggcgagaa gccggcctca accagcaact
     3301 caatattgcg ctgatctccg aagcccggca ccacgccgcc gtagccgctg gggtcgcagc
     3361 ccgccatcag cagtccgccg gcggcctcaa actcgcgctc aaagttcatc tcttttttca
     3421 gcatgggccc gcccaggtcg cgctccgcca tctgcgagcg gcctaacaga tactcctttg
     3481 cggcttgcgg cgagagcgtc ttgagagccg cgctctcgcg ctccatgggc gggcgattcg
     3541 acccgaaggt ctcaatcacc gccagcgtcg aggtaatggc cacgtggtgc gc
//
LOCUS       DASWZT010000196        11674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_11192, whole genome shotgun sequence.
ACCESSION   DASWZT010000196 DASWZT010000000
VERSION     DASWZT010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11674
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11192"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VGE92_05205"
     CDS             <1..597
                     /locus_tag="VGE92_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HEY1313250.1"
                     /translation="DWHVASRLELKFEKLYLKLLLPRARHSTRGASKRYAGLRYRADA
                     AGIEFVGMEVVRSDWTALAKEVQRELYQRLFMDQAVDVYLADVVRRVRSGDFDDALVY
                     RKNLRKGAEEYTATTPPHVVAARKSKQPPERLVSYVITTAGPEPVDNIQHGIDREHYV
                     MKQVKPVAEPVLAALGLDFERVIGDSRQIELYSLFDES"
     gene            complement(616..2055)
                     /locus_tag="VGE92_05210"
     CDS             complement(616..2055)
                     /locus_tag="VGE92_05210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY1313251.1"
                     /translation="MTGRKRSMPMMCLLLLAGCRVGPDYRTPANPQGADAPLVSRVAT
                     AETTEQPPDEWWRLYNDTRLDALLQEAFGANVDLAAAEANLSAAAALLSAARSGRYPD
                     TRASVGGIYGRDAVTDEILELGGHPPQSTWILDDLFEVSYEVDLFGRVRRSVEAARAN
                     AQSSAAARDSLKVVIAAETARAYAQICALGEQLQVARHSLQVVSSEADITARRHDAGA
                     NSEFDVVRAEGLVAQTRSAIAPLEGQRRAALFQLAALLGRTPVNAPVDVQVCESPPQL
                     STLMPIGDGATLLKRRPDVRQAERLLAAATARIGIATADLYPKVKLSGLYGAVAAQPS
                     NLTTNLGLTWAIGPSISWSFPNQALPRARIRQAKANASAALAGFDSVVLTALKETEQS
                     LALYAAAIEYRRSLAEAQGKAHKAFEMAHDQFLAGSVSNLDLLSAEQALIAADAAVAA
                     SDSRLVQDQIAVFKSLGGGWRYAEPLPPT"
     gene            complement(2052..3593)
                     /locus_tag="VGE92_05215"
     CDS             complement(2052..3593)
                     /locus_tag="VGE92_05215"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1313252.1"
                     /translation="MGLLAVLMGTFISTLNGRLSTFGLADIRGAVHAGFDDGAWITTA
                     QTVAQMLVAPAAVWMGSVFGPRRILLIAALAFGLISLATPFSTSLSMLLTLQFAGGLA
                     SGFFVPLTLSFVLLNTPPKYWAYGIALYALNLELSLNISASLEGWYVDHQSWRWIFWQ
                     NAPLAAVMALCLHFGVRPKPPLPTRPQGDWFGLVSGGLGLALIYAALDQGNRLDWLNS
                     GLIWGLLLAGTVLFAGFIVHEARSANPLLNLRVAFADPLPRLLLLIALLRLTILSTAY
                     LIPLYLGAVRGFRALEVGQTLIWIAAPQLIICPLAGLLLRRTDPRLVASVGFIFISIA
                     CLMVAYNLTPIWGSEQFLPSQLLQAVGQSFALSGVLFFAVLHLRPQDALTFGASVQVA
                     RLLGGEIGTAFVVTFARVRGQVASNLIGLHVQHGDGLVVQRLQSYAAATARAGDPASG
                     ASRAASVLGSLVRGMATTQAVIDTFVAVGVLTAIALILAVAHKPAPLGPASARPLFGP
                     RNTAT"
     gene            complement(3623..4702)
                     /locus_tag="VGE92_05220"
     CDS             complement(3623..4702)
                     /locus_tag="VGE92_05220"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEY1313253.1"
                     /translation="MSDLKFNIDWRTLPEVVWRALVFIIAVAILIIVATRWNYWQGGA
                     GWQTTDDAYLQADVTPIATKVSGYVRDLPVEDYEHVHSGQLLAQLVDVDYRAAVAQAE
                     ANVASAVAQADALKAQRELQAANVQAAQAVIRATAAAAQQNSRDLARQQRLLETGSSS
                     AEAQEKLDTVRAQLSAQLAQNRAQAQAAAQQLGVLAAQEAQAQAAIAAQRANLQIATL
                     NLGYTRITATQDGVVGQRQIKPGQYVGIGTQITTLIPLPHIWVIANYRETQLTHMAVG
                     QKAEITVDTFPGAKLRGHVLAFAPASGSQFALLPPDNATGNFTKVVQRIAVKIVIDDA
                     AGLGPRLFPGMSVEARVDARDSAHR"
     gene            complement(4710..5099)
                     /locus_tag="VGE92_05225"
     CDS             complement(4710..5099)
                     /locus_tag="VGE92_05225"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY1313254.1"
                     /translation="MAHVTVTSEYHYAQQIASGHHRLTADEPIALGGSDSGASPYELL
                     LASLGACTSITLRMYAGRKEWELGKITVGLRYTANDDKKGHIDRRLSFSKPLSAEQTK
                     RLLEIAAKTPVTLTLSPGVTIDTTVMQ"
     gene            5180..6955
                     /locus_tag="VGE92_05230"
     CDS             5180..6955
                     /locus_tag="VGE92_05230"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEY1313255.1"
                     /translation="MTRRIPMYSFNALPAVMMLSLASVPALAQPPGTRDADAALKNIY
                     SAEWAWREAQLPDDEDSQKPIADHLPDVGPQAQDARLQYWLDVRRRLDAIPRGNLSLA
                     EQLNYDVYSPQIDALIANQRFRDYEMPANSDTTFWTNLGYTARRTFRSLQDYRNWLAQ
                     MRDVPRYFREQTAEMRAGLKRGFTPSRITMQGRDSSLSAVTEVAAEASLYYAPFKDMT
                     GIPADEQTRLRAEAVKAIQESVQPAYRELLKFMRTEYLPQTRTTLAAQDLPDGRHYYQ
                     AKIREFTTLDMDPSAIHALGVREVARLREQMIAAMLETGFKQQLPEFLLSLRTDPRFY
                     AKTPEELLMRGAWIAKQFDGKAAKFFGYLPRARFAIRPVPDDLAPFYTAGRGGPGVYL
                     INTYDLPSRPLYSLTALTLHESAPGHAFQMPIAMEHTSQPEFRQHTYISAFGEGWALY
                     CEKLGEEMGMYDTPYDRFGMLGYQIWRAARLVVDTGIHSQGWTRERAIAYFRENTPLA
                     DREIQTEIDRYIAWPAQALSYYLGEMAILDARAKAERALGERFNIRAFHDAVLELGSV
                     PLPVLASRIDRFVAEGGKGPYPQME"
     gene            6963..7796
                     /locus_tag="VGE92_05235"
     CDS             6963..7796
                     /locus_tag="VGE92_05235"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: protein motif:HMM:NF014650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HEY1313256.1"
                     /translation="MRDHLHAKFGKLLLTAIIAAAGAAAAASGADRGSHANKSSSDSE
                     PDQELIITGRDALRRIVQQQCVVNWTQKHDPAPCERVFLPDSKTERAGYAVLADRKGG
                     AHYLLIPTQTMVGIDSEELLDPDLPNYFAEAWHARDLITAFVGHEVPRTAIGLAINTA
                     HSRSQDQFHIHIECLRPDVAESLSAAAPRVTDTWSPVIVAGSTYQAQRILSDGLDGSN
                     LFEMLARLKPEVRHHMSDYTLVVAGMRFKDGPGFIVLTGTGPTGELLLDAGCSVAGAG
                     G"
     gene            complement(7801..8643)
                     /locus_tag="VGE92_05240"
     CDS             complement(7801..8643)
                     /locus_tag="VGE92_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY1313257.1"
                     /translation="MNHLSRKPLWCLLVLSALWASAAVRADALRAVDVLRMGGCGGEL
                     PAARPLQHNAVLDRTAELWSGGQTPGAAADRSGYSAQAINGVHIRGPETATLDLLKRS
                     GCRTVADKSLHDIGLYRHGADTWLVLASAYVVPTSSEAPALAKRTLELVNDVRAHGTR
                     CGERPFGPVPPVRLSGTLAEVAYGHAIDMARHDYFEHQDRAGHSPADRVRAVGYREKL
                     VGENIAYGPQSAEEVVQGWLESPGHCENIMDARFAEMGIGYAPGQASKHGLYWVQLLA
                     APRA"
     gene            8749..9942
                     /locus_tag="VGE92_05245"
     CDS             8749..9942
                     /locus_tag="VGE92_05245"
                     /inference="COORDINATES: protein motif:HMM:NF020505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monodechloroaminopyrrolnitrin synthase PrnB
                     family protein"
                     /protein_id="HEY1313258.1"
                     /translation="MSSRAEAFEQWIRTSFVQMNTELENLYFAQPNRARVVGCGDALK
                     AALRDEGHTYVVALLAEGNTGEGFDSAYGVLGSVGLYLGALRRHELTNPAREERSPFR
                     EASSLALHVGSSLGMAPRFSTGHLATRNRAVAGVRKSFTSLRDEFLFIDENTRGILSL
                     QRAADALTSIVHLGVSSPVADVMFEVAAQALRDVMRFNGRLIDQLDVDRFFYSVRPYY
                     KPYRVGRQEYRGANAGDFSGINELDLLLGLCRANDPYYAQLLVDKMLFMLPGDQGRLR
                     DCMTRTSLLDELLTLVDAHSQADWFQQNARAYLEVCDLFGQAAAQHHDGLVRKFIERP
                     AGELVDEELAGITASGPPLPVLLRSLEVLRDLRLAADRRDISTRHGDLQRLRGVVRSV
                     ACSTA"
     gene            10193..11311
                     /locus_tag="VGE92_05250"
     CDS             10193..11311
                     /locus_tag="VGE92_05250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY1313259.1"
                     /translation="MTDEQAKSYLTKLLTAMTKASGSDLFISNEFPPSMKAHGTMRPM
                     TSQKLAGDFTRQLAHCLMNERQREEFAKELECNFAISIPGVARFRVNVFVQQQQVGMV
                     IRTIPAEIPDLASLELPEILKDIVMTNRGLVLVVGATGSGKSTSLAAMIDHRNRSTPG
                     HIITVEDPVEFVHQSHQSLVTHREVGIDTRSWHHALKNTLRQAPDVILIGEIRDAETM
                     EHAVAFAETGHLCLGTLHANSANQTIDRIINFFSEERRKQLLMDLSSNLKAIVSQRLI
                     RKVDGKGRKAAMEILINTPIIADKILKGDFHEIKAIMSKSRELGMRTFDWSLFELYNA
                     GDISYDEAIRNADSANELRLNIKLNGTREAPALAVIAS"
     gene            complement(11330..>11674)
                     /locus_tag="VGE92_05255"
     CDS             complement(11330..>11674)
                     /locus_tag="VGE92_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313260.1"
                     /translation="TVQHVHMPSVLNENPVIKERRTNLADVLKLALEREPNRHDLRMK
                     LLELYYTQASTNRNGFLEVVQKLAQDRDFRQSAEWEKIASMGRQIASDSPLFIDELAG
                     EDDDLSIERKSA"
BASE COUNT         2282 a         3615 c         3612 g         2165 t
ORIGIN      
        1 gactggcacg tggcgagccg actcgaactg aagttcgaga agctttacct gaaattattg
       61 ttgccgcggg cgcgccatag tacgcgcggt gcgagcaagc gttacgcggg tctgcgttac
      121 cgcgcggacg cggcgggcat cgaattcgtc ggtatggaag tggtgcgtag cgactggacc
      181 gctcttgcca aggaagtgca acgcgagctc tatcagcgcc tgttcatgga ccaggccgtg
      241 gatgtctatc ttgccgacgt agtgcggcgg gtcaggagcg gtgatttcga cgacgcattg
      301 gtgtatcgca aaaatttgcg caagggggcc gaggaataca ccgccacgac gccgccgcat
      361 gtcgtggcgg cgcgaaagtc caagcagccg cccgagcggc tggtgagcta cgtgatcacc
      421 acggcggggc ccgagccggt ggacaacatt cagcacggca tcgatcgcga acactacgtg
      481 atgaagcaag tgaagccggt tgcagaaccc gtgcttgctg cgttgggcct cgatttcgaa
      541 cgcgtcatcg gcgacagccg gcaaatcgag ctgtactcgc tgttcgacga atcgtgagcg
      601 cgtggagcag gctcgctagg tgggtggaag cggctctgcg tagcgccaac cgccgccgag
      661 cgacttgaag accgcgatct ggtcctgaac cagcctcgag tccgatgctg ccacggccgc
      721 gtcggcggca atcagcgctt gttccgcgga cagcaggtcg agattgctga cagaacctgc
      781 gaggaattga tcatgtgcca tttcgaaggc tttgtgagcc ttcccctgtg cttcggcaag
      841 cgaccgacgg tattcgatcg ctgcagcgta gagcgcgagc gactgttcgg tttctttcag
      901 cgctgtgagc accacggagt cgaacccagc caaagcggct gacgcattcg cctttgcctg
      961 acggattcgc gcacgtggca gcgcctgatt gggaaagctc caactgatgc tcggtccaat
     1021 tgcccaagtg agaccgagat tggtggtcaa gttcgatggc tgggcggcga ctgccccata
     1081 cagtcccgat aacttgactt tcggatacag atcggcagtt gcaatgccga tgcgtgccgt
     1141 ggcggccgcc agcaagcgct cggcttgacg cacatccggt cgacgcttga gcagggtcgc
     1201 gccgtctccg atcggcatca aggtgctcaa ttgcggcgga ctctcgcaga cttgaacgtc
     1261 cacgggcgca ttcaccggag tgcgcccgag gagtgccgca agttggaaca gcgccgcacg
     1321 ccgctggcct tcgagcggag caatcgccga gcgtgtttgc gcgaccagac cctcggccct
     1381 caccacgtca aattcggagt tggcaccggc gtcgtgacga cgcgctgtga tgtccgcctc
     1441 actgctgaca acctgcaatg aatgccgcgc gacctgaagt tgttcgccaa gcgcgcaaat
     1501 ctgcgcgtaa gcgcgcgccg tctcggccgc aatgaccact ttcaagctgt cgcgggccgc
     1561 ggcgctcgat tgtgcgttgg cgcgcgcggc ctcgaccgag cgccgcaccc ggccgaacaa
     1621 atccacctca taggacacct cgaaaagatc gtccaagatc caggtcgatt gcgggggatg
     1681 ccccccgagt tcgagaattt catccgtcac ggcatcgcgg ccgtagatgc cgcccacgct
     1741 ggcgcgcgtg tctggatagc ggccgctgcg cgccgcactc aacagggccg cggccgccga
     1801 gaggttcgcc tcggccgcgg cgagatcgac gttggcgccg aaggcctcct gcaacagcgc
     1861 atcgagccgg gtatcgttgt aaagcctcca ccactcgtcc ggcggctgct ccgtggtctc
     1921 cgcagtggct acccgcgaaa ccagcggcgc gtcggcccct tgcggattgg cgggcgtccg
     1981 gtagtccggt cccacacggc accctgcaag caacagcaga cacatcatcg gcattgagcg
     2041 cttgcggccg gtcatgtcgc ggtatttcgc ggcccgaaca agggacgtgc ggaggcgggt
     2101 ccaagcggcg cgggcttgtg cgccacggcc aggatcaatg caatcgcggt gaggacgcca
     2161 acggcgacaa atgtatcgat cactgcctga gttgtcgcca tgccgcgcac cagcgatccc
     2221 agcacgcttg cggcgcgcga ggcgcccgag gcgggatcgc cggcccgcgc ggtggcggcc
     2281 gcataactct ggagtcgctg aaccaccagt ccgtcgccat gttgcacgtg taaaccgatc
     2341 aggttcgatg cgacttgacc gcgtacgcgc gcgaatgtga cgacgaatgc cgtaccgatt
     2401 tcgccgccga gcagacgggc cacttggacc gatgcgccga acgtcagcgc gtcctgcggc
     2461 cgcagatgca agacggcgaa gaacaggacc cccgaaagtg caaagctctg gcctaccgcc
     2521 tgcaagagtt gcgaaggaag aaactgctcc gatccccaaa ttggcgtcag gttgtaggcc
     2581 accatcaggc aggcgatgct gatgaagatg aaaccgacgg aagccacgag gcgcgggtcg
     2641 gtccgacgca gcaagagacc tgccagcggg cagataatca gctgcggggc cgcgatccag
     2701 atcagggtct gacccacttc gagagcacgg aaaccgcgca ccgcaccgag atacaaggga
     2761 atcaggtaag ccgtcgacag tatcgtcagg cgcaatagcg cgatcagcag caatagccgc
     2821 ggcagcggat ccgcgaaggc aactctgaga ttgagcagcg gattggccga gcgcgcctca
     2881 tgcacgatga aaccggcgaa cagcaccgtg cccgcgagca gcagccccca gatcaagccg
     2941 gaattcagcc agtcgagccg attgccttga tcgagcgctg cataaatcag agccaagccc
     3001 aatcccccgg aaaccaggcc aaaccagtcg ccctgagggc gagtcggcaa cggcggtttt
     3061 gggcgcaccc cgaaatgcag gcacagtgcc atcacagccg cgagcggcgc gttctgccag
     3121 aaaatccagc gccacgattg atggtcgaca taccaaccct cgagcgaggc ggaaatgttc
     3181 agcgataatt cgagattcag cgcgtacagc gcgatgccgt aggcccagta tttcggcgga
     3241 gtattcagca gcacgaaact caacgtcagc ggcacaaaga atcccgaggc caagccgccg
     3301 gcaaactgca atgtcagcag catgctcaag ctcgttgaga acggtgtcgc gagggatatc
     3361 agaccgaagg ccaatgcggc gatgagcaga attcttctcg ggccgaacac cgaacccatc
     3421 cacaccgccg ccggcgcaac cagcatctgc gccaccgtct gtgcggtcgt gatccatgcg
     3481 ccgtcatcaa agcccgcgtg gacggcaccg cgaatatcgg caagcccgaa agtcgacaaa
     3541 cggccgttga gcgtcgagat gaatgttccc atcagcacgg cgagtaaacc caaccatggt
     3601 atcttcgctt gacccgcctc gctcatcggt gcgcgctgtc ccgcgcgtca accctggctt
     3661 cgacggacat gcctgggaag agccgcggtc caagcccagc ggcgtcgtcg atcacgatct
     3721 taacagcgat acgctgcacc actttggtga agttgccggt ggcattgtcc ggcggcagca
     3781 aggcgaattg cgaccccgac gcgggcgcga acgcgagcac atggccgcgc aacttggcgc
     3841 cagggaatgt atctaccgtt atctcggctt tctggcctac ggccatatgc gtcagttggg
     3901 tttcccggta gttggcgatt acccaaatgt gcggcaacgg aatcagagtc gtgatttgcg
     3961 ttccgatgcc gacatattgg ccgggcttga tctggcgctg tcccacgacc ccgtcctggg
     4021 tggcggtgat ccgggtgtag cccagattca gtgtcgctat ttgcaaattc gcacgttgtg
     4081 cggctattgc cgcttgcgcc tgtgcctcct gagccgccaa cacgccgagc tgttgcgccg
     4141 ccgcctgggc ttgagcccgg ttttgcgcca gctgcgcact gagctgggcg cgcacggtat
     4201 cgagcttctc ttgagcttcg gccgaggagg aacccgtctc gagcagccgc tgttggcgcg
     4261 ctaggtcgcg gctgttctgt tgcgccgccg ccgccgtcgc cctgatcacc gcctgggcgg
     4321 cctgaacatt ggccgcttgc agctcgcgtt gcgccttcaa ggcgtcggcc tgggccacag
     4381 cggacgctac gttggcttcg gcctgcgcca cggcggcacg ataatcgacg tccacgagtt
     4441 gcgcgaggag ctgcccgcta tgcacatgtt cataatcctc gaccggcagg tcgcgaacat
     4501 atcctgaaac tttggtggcg atgggtgtga catccgcctg cagataggcg tcgtcggttg
     4561 tctgccaacc ggcgccccct tgccaataat tccagcgcgt tgcgacgatg atcaaaatgg
     4621 cgaccgcgat gatgaacacg agcgcccgcc agacaacctc gggaagtgtg cgccaatcga
     4681 tattgaactt gaggtcgctc aagaagacat tactgcatga cggtggtgtc gatcgtgact
     4741 cctggcgaca aggtgagagt gacgggcgtc ttcgccgcaa tttccagaag acgcttcgtc
     4801 tgttcggcgc taagcggttt tgaaaacgac agccgccggt cgatgtgacc cttcttgtcg
     4861 tcgttcgcgg tatagcgaag tccgaccgtg attttaccga gctcccattc cttgcgaccg
     4921 gcgtacatcc gcagcgtgat ggacgtacag gcgccgagac tggcgagcag caactcgtag
     4981 ggggaagcac cggaatcgct tcctcccagc gcgatcggct cgtccgccgt caggcggtgg
     5041 tgtcccgatg cgatctgttg agcgtagtga tattcgctgg tgacagtcac gtgtgccata
     5101 aaatctccac aatttttata taatacaatc ggttagagtc taagtcagtt attgtcaatt
     5161 cacaacgagc gccgtgcgca tgacccgaag gattcccatg tactcattca atgcattgcc
     5221 ggcagtaatg atgctgtcgc tggcgtcggt tcccgcgttg gcgcagccgc cgggcacgcg
     5281 cgacgccgat gccgcactca agaacattta cagcgccgaa tgggcatggc gcgaggcgca
     5341 gctacccgat gatgaggata gccagaagcc catcgcggac cacctgcccg acgtcggccc
     5401 gcaggcacag gatgcgcgcc ttcaatattg gctcgacgtg cgccgcaggc tcgatgcgat
     5461 cccacgcgga aatttgtcgc ttgccgagca gctgaattac gacgtgtaca gcccgcaaat
     5521 cgacgcattg atcgcgaatc agcgctttcg cgactatgag atgccggcca attccgatac
     5581 cacgttctgg accaaccttg gctacacggc acggcgcacg tttcgctcgc tgcaggatta
     5641 ccgcaattgg ttggcgcaga tgcgcgacgt cccgcgatat tttcgcgagc aaaccgctga
     5701 aatgcgcgcc ggcctcaaac gcgggtttac gccgtcgcga atcaccatgc agggccgcga
     5761 ctcctcgttg agcgccgtga cggaggtcgc cgcggaggcc agcctctact acgctccgtt
     5821 caaggacatg accggtatac ctgccgacga acaaacccgc ttgcgcgctg aggccgtgaa
     5881 agcgattcag gagtcggtac aacctgcgta ccgagagttg ttgaaattca tgcggaccga
     5941 gtacctgccg caaacgcgca cgaccctggc ggcgcaagac ttgcccgacg ggagacatta
     6001 ttaccaggcg aagattcgcg agttcaccac gctcgacatg gatccttccg ccatccacgc
     6061 gttgggtgtg cgtgaagttg cacggttgcg cgaacagatg atcgccgcga tgttggaaac
     6121 gggcttcaaa cagcaattac ccgagtttct gctgtcgctg cgcaccgatc cgcggttcta
     6181 tgccaagaca ccggaagagt tgctgatgcg cggcgcttgg atcgccaagc agttcgacgg
     6241 caaggcagcg aagtttttcg gctacctgcc gcgtgcgcgc ttcgcgatcc ggcccgtccc
     6301 ggatgacctg gcgcccttct acaccgccgg ccgcggcggt ccgggggttt atctgatcaa
     6361 cacctacgac ctgccaagcc gtcctctgta cagcctgacc gccttgactc tgcacgagtc
     6421 ggctccgggc cacgcgttcc agatgcccat cgcgatggag cacaccagcc aaccggaatt
     6481 tcggcaacat acctacatct ccgcctttgg cgagggctgg gcgttgtatt gcgaaaagct
     6541 cggcgaggaa atgggaatgt acgatacgcc ctatgacagg ttcggtatgc tcggttacca
     6601 gatctggcgc gcggcacgcc tggtggtcga tacgggaatc cattcacagg ggtggacacg
     6661 cgagcgggcg atcgcctact tccgcgaaaa taccccgctg gcggatcgcg agatccaaac
     6721 cgagatcgat cgatacatcg cgtggccggc gcaggcgttg tcgtattatc tgggcgagat
     6781 ggcaatcctc gacgcgcgag ccaaggcgga gagggctctc ggagagcgct tcaatattcg
     6841 cgcgttccat gacgcggtgc tcgaacttgg ctccgtgccg ctgccggtgc tggcctcgcg
     6901 catcgatcga tttgtcgccg agggcggaaa aggcccgtat ccgcagatgg agtagtcatc
     6961 ttatgagaga tcacctgcac gcgaagttcg gcaaactgct gttgaccgca atcatcgcgg
     7021 ctgcgggcgc cgctgcggcg gcgtcgggcg ccgaccgagg ttcgcatgcc aacaaatcgt
     7081 cttccgactc ggagcctgat caagagttga tcatcaccgg ccgtgacgcc ctgcggcgga
     7141 tcgtgcagca acaatgtgtg gtgaattgga cgcagaaaca cgatccggcg ccatgcgagc
     7201 gtgtatttct acccgattcc aaaactgaac gcgccggata cgcggtactc gcggatcgca
     7261 aaggcggtgc gcactatctg ttgattccga cccaaaccat ggtcggcatc gacagcgagg
     7321 agttgctgga ccccgatctg ccgaactact ttgcggaggc ctggcatgcg cgggacctga
     7381 tcacggcgtt cgtcggccac gaggtgccga gaacggcgat cggactcgcg atcaacaccg
     7441 ctcattcgcg aagccaagat cagtttcaca ttcacatcga gtgcctgcgc ccggatgtcg
     7501 ccgaatcctt gagcgccgcc gcgccgcgag taaccgatac ctggtcgccg gtcattgtcg
     7561 caggatcgac ttaccaggcg cagagaattt tgagcgatgg attggatgga tccaatctat
     7621 tcgagatgtt ggcaagactg aagcccgaag tgcggcatca catgagcgat tacacgttgg
     7681 tcgtcgcggg tatgcgcttc aaggacgggc ccgggttcat cgtgcttacc ggtaccggcc
     7741 ctaccgggga gttgctgctg gatgcagggt gctcggttgc cggcgccggc ggctaaagcg
     7801 tcaagcgcgc ggcgccgcca acaactgcac ccaatagagg ccatgtttgg aagcctggcc
     7861 gggcgcatag ccgatgccca tttcggcgaa tcgcgcgtcc atgatgttct cgcaatgtcc
     7921 gggactctcg agccagcctt gtacgacttc ttcggccgat tgagggccgt aggcgatgtt
     7981 ctcgccgact agtttctcgc ggtagccgac cgctcggaca cgatccgccg gcgagtgtcc
     8041 cgcccgatcc tggtgctcga aataatcgtg ccgggccatg tcgatggcat gtccgtacgc
     8101 gacctctgcc aaggttcccg acagccgcac cgggggcacc ggtccgaacg ggcgctcgcc
     8161 acagcgagtg ccgtgcgcgc gcacgtcatt cacgagctcg agagtgcgct tcgcaagcgc
     8221 cggcgcctcg gaactcgtcg gcaccacata tgccgacgcc agcaccagcc aggtatccgc
     8281 gccgtgacga tataggccga tgtcatgcaa ggatttgtcg gcgacggtgc ggcatcccga
     8341 gcgcttgagc aaatcgagcg tcgctgtctc tggacctcga atgtgtaccc cgttgatggc
     8401 ttgggccgaa tagccgctgc gatccgcggc ggctccgggc gtttggccgc cggaccacag
     8461 ttcggccgtg cggtccagca cggcgttatg ttgcagcggc cgcgcggccg gcagctcgcc
     8521 gccgcagccc cccatccgca gcacgtcgac ggcacgcagc gcgtccgccc gcacggcggc
     8581 gctggcccac agcgcgctca agacgagcaa gcaccacaac ggtttgcgag acaggtgatt
     8641 catattcaaa ggcaacgcgc gaccggcgca tcggatgacc cgctgccggc ggtaccacat
     8701 cacacaatcg acgggtcatg ggcgattgca ggcgctacaa tccccgcaat gagcagcagg
     8761 gcagaagcat tcgaacagtg gatccgaacg tcctttgttc agatgaatac cgaactcgag
     8821 aatttatact tcgcgcagcc gaatcgtgcg cgggtggtgg gctgcggaga tgcgctcaag
     8881 gcggcgctgc gcgacgaagg ccacacttac gtcgtcgcgt tgctggccga aggcaacacc
     8941 ggcgaagggt tcgacagcgc ctacggcgtt ctgggcagtg tcggtttgta tctgggtgca
     9001 ttgcggcgtc acgaactcac caacccggca agagaggagc gttcgccgtt ccgcgaagcc
     9061 tcgtctctgg cattgcacgt cggctcatcg ctcggcatgg cgccgcgttt ctcgacgggc
     9121 catctcgcga cgcgcaaccg ggccgtcgcc ggcgtgcgca agagcttcac ctcgctgcgc
     9181 gatgaattcc tgttcatcga cgagaatacg cgcgggattc tatcgctgca gcgcgcagcg
     9241 gatgcactca ccagcatagt gcatctgggc gtgtccagcc ccgtggctga cgtgatgttc
     9301 gaggtggcgg cgcaggcgct tcgcgacgtc atgcgcttca acggccggct gatcgatcag
     9361 ctggacgtgg atcgattctt ctattcggtg cgtccctatt acaaacccta ccgcgtcggt
     9421 cgtcaggagt atcgcggcgc aaacgccggc gacttttcgg gcatcaatga gctcgacttg
     9481 ctgttgggct tgtgcagagc caatgatcct tactacgccc agctgctggt cgacaagatg
     9541 ctgttcatgc tgcctggcga ccaggggcgc ttgcgcgatt gcatgacgcg caccagtttg
     9601 ctcgatgagc tgctgacgct ggtcgacgcg cattcgcagg ccgactggtt tcagcagaac
     9661 gcgcgtgcct acctcgaggt gtgcgatctg ttcgggcagg ccgctgcgca gcaccacgac
     9721 ggcttggtca gaaaattcat cgagcggccc gccggcgagc tcgtcgatga ggagctcgcc
     9781 gggatcaccg ccagcggacc gccgctgccg gtcctgctgc gctcgctcga agtgctgcgc
     9841 gacttgaggc tcgcggccga tcggcgcgac atcagcacgc ggcacggtga tttgcagcgg
     9901 ctgcgcggcg tcgttcgcag cgtcgcctgc agcacagcct gattctcggg cgtgagccaa
     9961 tcggcggact caccggccgg cgccacggca aaatgcgccg atgcgcgacc gagtggttgt
    10021 ttgcagcgcc atgcctccga cgatgcgtat tggctgtcca cggacgtgcg ccgcggcgtt
    10081 gcaacgcgca gcaccgggca atagctgtca tcgcgtttga taactccatc gcggacggct
    10141 gcgagttcgc ccggatatag tcggctttga accaacaacg ggcggcatgc cgatgaccga
    10201 cgagcaagca aaatcctact taaccaaatt gctgacggcc atgaccaagg catcgggctc
    10261 ggatttgttc atttccaacg aattcccgcc aagcatgaag gctcacggca cgatgcggcc
    10321 gatgacctcc caaaaactcg cgggcgattt cacacggcag ttggctcact gcctgatgaa
    10381 cgagcgccaa cgcgaggagt ttgccaagga actcgagtgc aatttcgcga tttcgatccc
    10441 cggcgtcgcg cgctttcgcg tcaatgtgtt cgtccagcaa cagcaggtcg gcatggtcat
    10501 caggaccatt ccggccgaga tacccgattt agcttcgctg gagttgccgg agattctcaa
    10561 agatatcgtc atgaccaacc gcggattggt gttggtcgtg ggtgccaccg gttcgggcaa
    10621 gtcgacttcg ctggccgcca tgatcgatca tcgtaaccgc tcgacgcctg gccatatcat
    10681 caccgtcgag gatccggtcg aattcgtgca tcaatcgcac cagtccttgg tcacacaccg
    10741 tgaggtcggc atcgacaccc gctcttggca tcacgccctg aagaacacgc tgcgccaggc
    10801 gcccgacgtc atcctgatcg gcgagatacg cgacgccgaa acgatggagc atgcggtggc
    10861 gtttgccgaa accgggcatc tgtgcttggg cacgctgcat gcgaatagcg ccaatcagac
    10921 catcgatcgg atcatcaatt tcttctccga ggagcggcgc aagcaattgc tgatggacct
    10981 gtcgagcaat ctcaaagcta tcgtctcgca gcgcctgatc cgcaaggtcg atggcaaggg
    11041 acgcaaagcc gccatggaga tcttgatcaa cacgccgatc attgccgata agatcctcaa
    11101 gggggacttt cacgagatca aggccatcat gtcgaagtcg cgcgaattgg ggatgcgcac
    11161 tttcgattgg tcgttgttcg agctctacaa cgcgggcgat atttcctatg acgaagccat
    11221 ccgcaacgcc gactccgcga acgagctgcg cctgaatatc aagctcaacg ggacacgcga
    11281 ggcaccggca ctcgccgtca tcgcttcgta ggggttcggc agccgcgcgt caggcgctct
    11341 tgcgctcgat cgacagatcg tcgtcttcgc cggccaattc atcgatgaac aacgggctgt
    11401 cggaggcgat ctgccggccc atcgaggcga tcttttccca ttcggcactt tgccggaagt
    11461 cccgatcctg cgcgagtttc tgcaccacct cgagaaagcc attgcggttg gtgctcgcct
    11521 gcgtgtaata caactccaga agcttcatcc gcaggtcgtg ccgattgggc tcgcgctcga
    11581 gggcgagctt cagcacgtcg gcaagattcg tgcggcgttc tttgatcacc gggttttcgt
    11641 tgagcacgct cggcatatga acgtgctgca cggt
//
LOCUS       DASWZT010000197        16319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_49750, whole genome shotgun sequence.
ACCESSION   DASWZT010000197 DASWZT010000000
VERSION     DASWZT010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 16319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16319
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49750"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VGE92_05260"
     CDS             <1..466
                     /locus_tag="VGE92_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY1313261.1"
                     /translation="RDDHHESLRLWLRLLSCSTLIENHIRQRLQGEFATTLPRFDLMA
                     QLERCPEGLKMGELSKRLMVTGGNVTGITDLLEKEDLVARVTDAHDRRAFRVTLTPSG
                     QRLFKRMAAAHERWIIELFAGLPAKTKQQLAGLLNHLKDHVRHEHSRAEGSV"
     gene            463..1272
                     /locus_tag="VGE92_05265"
     CDS             463..1272
                     /locus_tag="VGE92_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HEY1313262.1"
                     /translation="MSLANCNPKNFLWEIHGRVGVITLNRPERKNPLTFDSYAELRDL
                     FRGLSAEPAVKAIVITGAGGNFCSGGDVHEIIGPLTRMDPPRLLDFTRLTGDLVMAMR
                     ECPQPIVAAVSGVCAGAGAIIAMAADLRFSTPDSRTAFLFVRVGLAGCDMGACAILPR
                     IIGHGRAAELLYTGRSMSAQEGSSWGFFNRLVEQSSLPAEAIEFAAVLANGPTIAHAM
                     TKRMLNQEWNLSLAAAIEAEAQAQAQCMQTEDFRRAYRAFAAKSTPQFEGN"
     gene            1280..2476
                     /locus_tag="VGE92_05270"
     CDS             1280..2476
                     /locus_tag="VGE92_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY1313263.1"
                     /translation="MSSAAHLQWPFFAPRHRELHQQVQDWCAVHLQATHGDDRQQVDA
                     HCIALVKRLGAAGILRHCVRGEDGGASQDFDVRSIAIVREALAQFDGLADFAFAMQGL
                     GSGALTLAGTARQRGHYLPRVARGEVCAAFALSEADAGSDVAGMQCRARREADEYVLD
                     GAKTWISNGGIADFYCVFARTQAASLRADGSAAATGISAFVIDADTPGLSVSERIDII
                     APHPMATLEFRGCRIPAGQRLGEEGEGFKIAMRTLDIFRTSVAAAALGFAQRALDEAL
                     GQVRGRRMFGRQLADFQMTQAALAEMATEVDAARLLTYRAAWLRDCGGRVTKEAAMAK
                     MTATETAQRVVDRALQMFGARGVVRGEVLERLYRDVRALRIYEGATEVQKLIIARELL
                     APTDSA"
     gene            2473..2892
                     /locus_tag="VGE92_05275"
     CDS             2473..2892
                     /locus_tag="VGE92_05275"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012429996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY1313264.1"
                     /translation="MTRDKRVPSIVQPAGWARPKGYSHAMTARGLGIFVAGQIGWDSG
                     GKFHSLRMADQVRQALANIVTILAEAGATPAHIVRLTWYVTSRDEYHEQLADIGAAYR
                     ATLGKHFPPMSVVQVVALMEKDARVEIEATAVLPDAG"
     gene            2953..3570
                     /locus_tag="VGE92_05280"
     CDS             2953..3570
                     /locus_tag="VGE92_05280"
                     /inference="COORDINATES: protein motif:HMM:NF020432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like protein"
                     /protein_id="HEY1313265.1"
                     /translation="MALPREPRIHPVAIASLRPTQITVGMREVKAKRKFWREQRKKAD
                     FLGKHLIPVIWGPKDEYYVIDHHHLTLALHQEGVKYIATTVVADLRRLPRDAFWVFLD
                     NRGWLHPFDARGRRRSYQDVPKRIMGLVDDPFRSLAGELRQSGGYAKDTTPFSEFLWA
                     DFLRRRLKRKFLEDDFPAALVRALKLAKSGEADFLPGWCGPIAGQ"
     gene            3574..5190
                     /locus_tag="VGE92_05285"
     CDS             3574..5190
                     /locus_tag="VGE92_05285"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEY1313266.1"
                     /translation="MRAVNSADALAGLSLAGLLLPEAVAYSGLANLPPQAGVIGLFAG
                     LLCYALIGSSRYAIVTATSSSAAVFAAATLALAANASGQRMAFASILVCATGIAFVLA
                     GCARLGAMSNLIARPVLRGFSFGLALVIAVKQWPHIVAIHTQSSDFLPLFSEILRHVR
                     EWQPVSLGVGVGALAGLFLLERFRHIPGVLLVIIAGIAAAPWLALRGVVLTGPIHLVL
                     ERPEFAMPAGQHWLPLVEFALALMFILYAESYSSIRTYALKHDDVVQPNRDLIALGAA
                     NILSAAFHGTPVGAGYSGTSANDAAGAQSRAAGVFAAATVLALVLLFLPWIERIPDPV
                     LAAIVIHAVSKSLRLATFRPYFRWQRDRLVTLTAVAAVILFGMLDGLLAAIAFSLGML
                     LHSLTSPRLSILGRVGAHDYVSVTRFPQAACTPGMLILRPEEPLFFANAEPLLAVARQ
                     RVLQQTDVKLVILSLEESPDLDGTALESLGEFAAWLTVRKIGLRVARLKESSRDALQR
                     ADFSQLPPAGLDYSSVDDAVTGSAAGGAAQ"
     gene            complement(5206..6720)
                     /locus_tag="VGE92_05290"
     CDS             complement(5206..6720)
                     /locus_tag="VGE92_05290"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1313267.1"
                     /translation="MLHLPMFWMAHAHGFVLAGMPMDPGMLAGMACIVGGIIAAGYGL
                     LPVPVVMGVLEPFEPPEDAPLTSAHWRLMLVLAVALIIDVMKPASLGFVTPGMRVEYG
                     IDRTTVALLPLSALIGTAVGSFVWGALADIYGRRASILLSSVMFVGTSICGAMPSFSW
                     NIAMCFLMGAAAGGMLPVAYALLAEIMPTRHRGWCLVLVGGIGTIGGYFAASELSALL
                     QPFFGWRIMWLLNLPTGLALISLSPLIPESARFLQHLGRLDDARTTLARFGALGKPRI
                     EASGSSGAEHQESLVRAAAHGLLGTTVALTLAALAWGFVNFGVLLWLPSALVAEGRSV
                     GLASAVIARSALFAAPTIILASYLYSTWSTKWSLIGSIALTTLGLLAVMLHGSAHWPI
                     LSNPILPLCLLIVGSSGMISMLLPYAAENYPLRVRGRATGWVAGWSKIGGVIAQVLGV
                     LGLVPALGAVAAFVAIPASLSLILIGCFGRETRGRDLRELEVRGTELRKALKRP"
     gene            complement(6814..8115)
                     /locus_tag="VGE92_05295"
     CDS             complement(6814..8115)
                     /locus_tag="VGE92_05295"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY1313268.1"
                     /translation="MRDLPNELAALADHLNIHHEAILIAWRRAILKDPDLTSGDSLPR
                     SELFDHIPALLRAFEIGLRGASSTAERKAEADAHDQAAAHGLQRWQQGYDLREVSREW
                     GKLNQCVLGELDRYATENSSISLDAMSRAREVWAILLSTGIETSVHQYFTLQQQEAVG
                     HVKDLEKALEQVQALEQERGAVWQEAAHDLRGNLGVVANVTVGLTRHGPSDAKREDFI
                     RILMRNVSSLHQLLDDVTNLARLQAGRESRRVEPLDATSVLESLCEGIRPLAQQRGLQ
                     LLAQGPRGLAIDGDAVKIRRIAQNLILNAVKYTREGCITVAWGDSNPGDLKRWMLSIS
                     DTGPGFHTASGQPLAAALDPAATPAASPPASGLASRPAAAVASGPDGELRSFLRGEAG
                     EGIGLSIVKRLCEMLDATIEMESVKGQGTTFRILFPRHYES"
     gene            complement(8118..8921)
                     /locus_tag="VGE92_05300"
     CDS             complement(8118..8921)
                     /locus_tag="VGE92_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY1313269.1"
                     /translation="MQFDTLLFERRDGIAHVTLNRPDRLNALNMALIADLRAAAAAIE
                     ADAEIRAVILGAAGRGFCSGADLMGSNFSGGAEARLGEVIGIELREHFNPMVNAWYQL
                     RVPVVVAVNGVAAGAGVSLALLGDIVLAARSATFLQLFAPKLGLMPDLGSTFHLPRLV
                     GTARAKGLALLGDALSAADAAGWGLIWGCVEDTALQAEAQAIARRLAAGPTQAFRRIK
                     AAFNTELPRTLSEQLALEADAQVELGNTRDFAEGVQAFRDKRAPKFTGN"
     gene            8999..9985
                     /locus_tag="VGE92_05305"
     CDS             8999..9985
                     /locus_tag="VGE92_05305"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEY1313270.1"
                     /translation="MPIPEILQGRLRMPVIGAPMFIVSTPRLVLAQCKAGIVGAFPSL
                     NARPAAQLDDWLAQITSELADYRRANPHLKVAPFAVNQIVHSSNDRLEHDVALCVKHR
                     VPIIITSLRPPSEVVAAVHGYGGVVFHDIINLRHAEKAAAQGVDGIIAVCAGAGGHAG
                     VLSPFALVKQIREVYAGTIILSGAMSSGADVLAALALGADLAYLGTRFIATEEANAPA
                     EYKQMLIESRGEDIVYTSLFSGVLGNYLRGSVTRAGLDPDKLPEADKAKMNFGSGGAS
                     ELKAWRDIWSAGQSVSGIHDVKAVGALIEGMAHEFNAARARVNAVQIADGPV"
     gene            9995..11020
                     /gene="holA"
                     /locus_tag="VGE92_05310"
     CDS             9995..11020
                     /gene="holA"
                     /locus_tag="VGE92_05310"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEY1313271.1"
                     /translation="MRLSADQLSAALSRGVAAPIYLVSGDEPLLVGEAADAIRAAART
                     AGYADRTVFFIDRSFSWDEFKHATQALSLFAERRLFELRMPSGKPDKGAQLLAEVATN
                     PPPDVVCLVLCDKLDKKASEAPWVRAVEKHGVWVPVWPVQSAALPAWLRERARQLHVQ
                     IEPAAAQLIVERVEGNLLAAKQELDKLCMLANGAPIDAELVLRSVGDSARYDVFQLAA
                     AAAAGEAARALRVLLGLKSEGVEPTLILWALVRELRGLWQARERDRLRSTVRGSGWNL
                     AATPSPRALSRLKQLPLEQLMKQASHADRVIKGLSAGEPWSALTGLAGGLAGALQATA
                     DSGRVRL"
     gene            11017..11664
                     /gene="nadD"
                     /locus_tag="VGE92_05315"
     CDS             11017..11664
                     /gene="nadD"
                     /locus_tag="VGE92_05315"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000839.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEY1313272.1"
                     /translation="MNPMGIFGGTFDPIHYAHLRTAFELQQALRLKEIRFLPAGNPPH
                     RERPIADAELRLQMVQLATAGQSGFVVDDREVRKQGPSYSVETLAELRHEYPDRSLCL
                     IVGMDAFLSLPKWHQWRDLLQLAHLVVAHRPGWRAPSMGPLGELLVDSGTGRIGDLHD
                     TRAGCIYIHAVTQLEISSTEVRQLISMGRDPRFLMPDAVRKLILDTHCYSKTRTT"
     gene            11664..12155
                     /gene="rsfS"
                     /locus_tag="VGE92_05320"
     CDS             11664..12155
                     /gene="rsfS"
                     /locus_tag="VGE92_05320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEY1313273.1"
                     /translation="MPVKKKSSNGKIKGRKKALRHSPAGKSKPAGDIKAARHPATAAA
                     RKPAKAGPSLKSVVIDALDDMKALEVKVLDVRGLTDVADYMVIASGTSDRHVRSVAQR
                     VVERTKEAGFRPHGVEGQQDSDWVLIDLNEMIVHVMLPRVREFYGLEKLWDITATKRA
                     ARA"
     gene            12171..12641
                     /gene="rlmH"
                     /locus_tag="VGE92_05325"
     CDS             12171..12641
                     /gene="rlmH"
                     /locus_tag="VGE92_05325"
                     /inference="COORDINATES: protein motif:HMM:NF000986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEY1313274.1"
                     /translation="MKCRLIAAGTRLPEWVNGGFREYQKRLRTPFVLELHEIAVATRR
                     AGENPQRAIQRESDDMLAALKQNDYVVALEIAAPSMSTEQLSAWLAERMRDGRSLALL
                     IGGPDGLSAQCRERADRSWSLSPLTLPHALVRVVVAEQLYRAMSLLAGHPYHRA"
     gene            12669..13271
                     /locus_tag="VGE92_05330"
     CDS             12669..13271
                     /locus_tag="VGE92_05330"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEY1313275.1"
                     /translation="MHSDFVYLASASPRRRELLQQIGVAFRTVGSNVDETALPGEAPL
                     DYVTRLAAAKAEAGWQQSRDPAQAPVLAADTAVVLDGSILGKPKDRQDAEAMLARLSG
                     RTHEVMTAVALRTAQGLRAKVCRSEVTFRPIAAAEARDYWETGESQDKAGAYAIQGRA
                     AVFIADLRGSYSGVMGLPLYETAELLSQAGVTWHGREARS"
     gene            13268..14737
                     /gene="rng"
                     /locus_tag="VGE92_05335"
     CDS             13268..14737
                     /gene="rng"
                     /locus_tag="VGE92_05335"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEY1313276.1"
                     /translation="MSTEILVNASTHEARAALVENGVLQEVYLERASRRGLISNIYKG
                     RVSRVLPGMQAAFIEIGMERTAFLHASDIFDARHVDTGIEQPRTENIRALVAEGNEIL
                     VQVVKDPMGTKGARLTTYITLPSRYLVYMPKGHGVGVSARIESEGERERLRAAVQAAT
                     ESGEQAGFIVRTAAEDAPQEALRADMLYLRKLWEFVRHNGLRTQPGNLVHADLPLHLR
                     VLRDLLRPEVDRVLIDQANAHREMREFAAAFMPQALQRLELYDGVRPLFELHHVEEEI
                     QKALERKVSLKSGGHLIIDQTEAMTTIDVNTGAFVGHRNLEETIFRTNLEAAVTIARQ
                     LRLRNLGGIIIIDFIDMEEPEHRRQVIQALERALGDDHVKTSISSVSPLGLVEMTRKR
                     TRESLEHMLCRPCPTCEGKGFVKTAETVCYEVFREIVRQSRQFECQQLMVLAHQDVIE
                     RLLDEESSALGELELVTGKPIRLQTEALYSVDQYDVVLM"
     gene            14738..>16319
                     /locus_tag="VGE92_05340"
     CDS             14738..>16319
                     /locus_tag="VGE92_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313277.1"
                     /translation="MSARKVGKILLTGFAGLLSLLLLALLAAKLMLDRAPAYEGEIKQ
                     WVFAQTGYHIDFANVSPSFRWYGPELHFEHLELRSKDDRRVLARAAGGRVAADIWQLL
                     RSGKLLAGRIQLDAPNLVIARLGPASFALASEIKLGGDNSSLEALTLNDLPVGKLVIR
                     DATITLQNWNGALPKLELRKVNLDVRRGDQGLALWVDARLPEALGGTLNFAGNARGHG
                     EFQMLRWSALARARDISFPGWRSLLPEYLSRLDAGDGAFDLAASGVGAMLERADLDFA
                     ATGVVTQLQEGPSAKFEQISGALSIVHSSDRWTLTGRRVRAVRQGRRDPDSEFNVSWR
                     GGDSGLLELRAAASYLRADALLPLAGLLPEKEIRERLQEIAPTGEWLDSFVALARNSA
                     QDPWRLQVRARFHEVGFAPVGRAPGLRGLSGSIAGNENGGHVKIERGAALFTWPAQLL
                     KPVGLDSLAATLYWKRTPDALLVASSDWTMANRDAGAHGKVAWQQPADGSSPLLTLAG
                     AVENGHAAETRSYFPRALI"
BASE COUNT         2892 a         5150 c         5405 g         2872 t
ORIGIN      
        1 gcgcgacgac catcatgagt cgttgcggct gtggctgcgg ctgctcagct gctcgacgct
       61 gatcgaaaac catatccggc agcgcctgca aggcgaattc gccaccacgc tgccgcgctt
      121 cgacttgatg gcgcagctcg aacgctgccc ggaaggactc aagatgggcg agttgtcgaa
      181 gcgcctgatg gtcacgggcg gcaatgtcac cggcatcacc gatttgctgg agaaagaaga
      241 tctggtcgcg cgcgtgaccg atgcgcacga tcggcgggcg tttcgcgtga cgctgacgcc
      301 gagcggtcaa cgtctcttca aaaggatggc cgcggcgcac gagcgctgga tcatcgagct
      361 attcgccggt ttgcctgcga agaccaagca acaactggcg gggctgctga accatctcaa
      421 ggaccacgtg cgtcacgaac actcccgggc ggagggctcc gtatgagtct cgcgaactgc
      481 aaccccaaga attttctctg ggaaatccac ggccgcgtcg gcgtcatcac gttgaatcgc
      541 ccggagcgca agaatccgct gaccttcgac tcgtatgcgg agttgcgtga cttgttccgc
      601 gggcttagcg ccgagcccgc cgtcaaagcc atcgtgatca cgggcgcggg cggcaatttc
      661 tgctccggcg gcgacgtgca cgagatcatc gggccgttga cgcgcatgga tccgccgcgg
      721 ctgctcgact tcacgcgcct gacgggcgat ctggtgatgg ccatgcgcga gtgtccgcag
      781 ccgatcgtgg cggccgtcag cggcgtgtgc gccggcgccg gcgccatcat cgccatggcc
      841 gccgacttgc gattttcgac gcccgacagc cgcaccgcat tcttgttcgt gcgcgtcggc
      901 ctcgcgggat gcgacatggg tgcgtgcgcc attctgccgc gcatcatcgg gcatggccgc
      961 gccgcggaac ttctctacac cggccgctcg atgagcgcgc aggaaggctc gagctggggt
     1021 tttttcaacc ggctggtcga gcaatcgagc cttcccgccg aggccatcga attcgccgcg
     1081 gtgctcgcga acgggccgac catcgcccat gccatgacca agcgcatgct gaaccaggaa
     1141 tggaatctat cgctcgcggc ggccatcgaa gccgaagcgc aggcgcaggc gcagtgcatg
     1201 caaaccgagg attttcggcg cgcctatcga gcgtttgcgg ccaagagcac acctcagttc
     1261 gaagggaact gacgcagcca tgagcagcgc agcccatttg cagtggccgt tcttcgcgcc
     1321 tcggcatcgc gagctgcacc aacaggtgca ggactggtgc gccgtccatc ttcaggcaac
     1381 ccacggcgat gaccggcagc aggtcgacgc gcactgcatc gctctggtga agaggctcgg
     1441 ggctgcggga attcttcgcc actgcgtgcg cggcgaggat ggcggtgcat cgcaggactt
     1501 cgacgtgcgc agcatcgcca tcgtgcgcga agcattggcg caattcgacg gcttggcgga
     1561 tttcgcgttt gccatgcagg gtctcggcag cggtgcgctc accctggcag gcaccgcccg
     1621 ccagcgagga cactacttgc cgcgcgttgc ccgcggcgag gtttgcgccg cgtttgccct
     1681 gtccgaggcg gacgcgggct cggatgtcgc gggcatgcaa tgccgcgctc ggcgcgaggc
     1741 ggacgagtat gtgctcgatg gcgcgaagac gtggatttcg aacggcggca tcgcggattt
     1801 ctactgtgtt tttgcgcgca cgcaggcggc cagcttgcgc gcggacggga gcgcggccgc
     1861 aaccggcatc agcgccttcg tgatcgacgc cgacacgccg ggtttgtcgg tgagcgagcg
     1921 catcgacatc attgcgccgc acccgatggc gacgctcgaa tttcgcggct gccgcattcc
     1981 cgccggccag cgcctcggcg aggagggcga gggcttcaag atcgcgatgc gcaccctcga
     2041 tatattccgc acctcggtcg ccgcggccgc gctcggcttc gcgcagcgcg cgctcgatga
     2101 ggcgctcggg caggtccggg gacggcgcat gttcggccgc cagttggcgg attttcaaat
     2161 gacgcaggcc gcgctcgcgg agatggcgac cgaggtggac gcggcacggc tattgaccta
     2221 tcgagcggcg tggctgcgcg actgcggcgg gcgggtcacg aaggaagcgg ccatggcgaa
     2281 aatgaccgcc accgaaacgg cgcagcgcgt ggtcgaccgc gcgctgcaaa tgttcggcgc
     2341 gcgcggcgtg gtacgcggtg aagtcttgga gcgcctgtat cgcgacgtgc gggcgctgcg
     2401 catttacgaa ggcgccaccg aagtgcagaa actgatcatc gcccgcgagt tgctcgcgcc
     2461 aacggacagc gcatgacccg ggacaaacgc gtaccgtcca tcgtgcaacc ggccggctgg
     2521 gcgcggccca agggctatag ccacgccatg accgctcggg gtctcggcat tttcgtggcc
     2581 ggacaaatcg gctgggacag cggcggcaaa tttcacagct tgcgcatggc ggaccaggtg
     2641 cggcaggcgc tcgccaatat cgtgacgatt ttggccgagg ccggggccac gccggcacat
     2701 atcgtgcgcc tcacctggta cgtgacgagc cgcgacgaat accacgagca actggccgat
     2761 atcggcgcgg cgtaccgcgc gacgctcggc aagcactttc cgccgatgtc ggtggtgcag
     2821 gtcgttgctc tgatggaaaa agacgcgcgc gtggagatcg aggcaacggc cgtgctgccg
     2881 gatgccggtt gaccctgggc ggcggccatc gtgatatctt ttcggagcgc gaatcaggcg
     2941 taggagcaaa caatggcgtt acccagggaa ccccggattc acccggtcgc gatcgcatcg
     3001 ctgcggccga cgcagatcac ggtcggcatg cgcgaagtca aggccaagcg caagttttgg
     3061 cgcgagcaac gcaagaaagc ggacttcttg ggcaagcact tgattcccgt catctggggc
     3121 ccgaaggacg agtactacgt catcgatcat catcatttga cgctggcgct gcatcaggag
     3181 ggtgtgaaat atatcgcgac cacggtggtg gcggatttgc gccgtctgcc gcgcgatgct
     3241 ttctgggtgt ttttggacaa ccgcggctgg ctgcatccct tcgatgctcg cggccgccgc
     3301 cgcagctatc aagacgtgcc gaagcggatc atgggtttgg tcgatgatcc gtttcgcagt
     3361 ctcgccgggg agttgcgcca aagcggcggc tatgcgaagg acacgacgcc gttcagcgaa
     3421 tttctgtggg cggattttct cagacgtcga ttgaagcgca agttcctgga ggatgatttc
     3481 ccggcggcac tggtgcgcgc attgaaactg gccaagagcg gcgaggcaga cttcctgccc
     3541 ggctggtgcg ggccgattgc cggacagtga gcgatgcgcg cggttaactc ggccgacgct
     3601 ctcgccggtc tgtccctggc cggcttgcta ttgcccgagg cggtggccta ttctgggctc
     3661 gcgaatctgc cgccgcaggc gggtgtcatc ggcctgttcg ccgggctgct ctgctatgcg
     3721 ctgatcggca gcagccgcta tgccatcgtc accgccacct catcgtcggc cgcggtattc
     3781 gccgcggcga cgctggcact cgccgccaat gcctcggggc agcgcatggc gttcgcatcg
     3841 atactcgtgt gtgcgaccgg catcgccttc gtgctcgccg gctgcgcgcg gctcggcgcc
     3901 atgtcgaacc tgatcgcgcg gcccgtgctg cgcggctttt cctttggcct cgcgctcgtc
     3961 atcgcggtga agcagtggcc gcacatcgtc gccatccaca cgcagagcag cgattttctg
     4021 ccgctgttct cggagattct gcgccatgtc cgcgagtggc agccggtgag tctcggcgtc
     4081 ggggtgggtg cccttgcggg cttgttcttg ctcgagcgct ttcgacatat tcccggcgtc
     4141 ctgctggtca tcatcgccgg cattgccgcg gcgccgtggc tggcgttgcg gggcgtggta
     4201 ctcaccggcc ccatccatct cgtcctggag cggccggaat ttgccatgcc tgcgggtcag
     4261 cactggttgc cgctggtgga gttcgcactg gcgctgatgt tcattctgta cgcggaatcc
     4321 tatagctcga tccgcaccta tgcgttgaag cacgacgacg tggtgcagcc gaatcgcgac
     4381 ctgatcgcgc tcggcgcggc caacattctg tcggctgctt ttcacggcac gccggtcggg
     4441 gcaggctatt cgggcacgtc ggccaacgac gcggccggcg cgcaatcgcg cgcggcgggc
     4501 gtgttcgccg ccgcgaccgt gctcgcgctc gtgctgctgt tcttgccgtg gatcgagcgc
     4561 attcccgacc cggtgctggc ggcgatcgtc atccatgcgg tcagcaagtc gttgcgcttg
     4621 gcgacgttcc gtccttactt tcgctggcag cgcgatcggc tggtcacgct cacggccgtg
     4681 gccgcggtca tcctgttcgg catgctcgac ggactgctcg ccgccatcgc gttcagcctg
     4741 gggatgctgc tgcattcgtt gacgagcccg cgtctttcga tcttgggccg cgtcggcgcg
     4801 cacgattacg tcagcgtcac gcgttttccg caggcggcct gcacgccggg catgctcatc
     4861 ctgcggcccg aggagccgct gttttttgcg aacgccgagc cgttgctcgc cgtcgccagg
     4921 caacgcgtgc tgcaacaaac cgacgtcaag ctcgtgatct tgagtctcga ggaatcgccg
     4981 gacttggacg gcacggctct ggaaagcctg ggtgaattcg ccgcctggct cacggtacgc
     5041 aagatcggtc tgcgtgtcgc aagactgaaa gagtcgagcc gcgatgcgtt gcagcgcgcc
     5101 gacttcagtc aactgccccc ggccggactc gactactcga gcgtggatga cgccgtcacc
     5161 ggcagcgccg cgggtggcgc ggcgcaatga accgccccgg gcagatcacg gccgcttaag
     5221 cgccttgcgc agttcagtgc cacggacttc gagttcgcgc agatcgcggc cccgtgtctc
     5281 acgaccaaag cagccgatga gtatcaacga gagcgaggcg ggtatggcga cgaacgcggc
     5341 gaccgcgccc aatgcaggga ccaagcccag cacgcccagc acctgcgcga tgacgccgcc
     5401 gatcttgctc cagcccgcca cccagccggt tgccctgcct cgcacgcgga gcggatagtt
     5461 ctcggcggcg tatggcagca gcatcgaaat catgccgctc gagccgacaa tcagcaggca
     5521 cagcggcaaa attggattgc tcaggatcgg ccagtgtgca ctcccgtgca gcattacggc
     5581 caacagaccc aaggtcgtga gagcgatgga gccgatcagc gaccacttcg tgctccaggt
     5641 gctgtaaaga taactggcaa gaatgatggt cggtgccgcg aacagtgcgg agcgtgcgat
     5701 aacggcgctc gccagaccga cgctgcggcc ttccgccacc agcgcgctgg gcagccataa
     5761 cagcacgccg aaattgacga aaccccaggc caaggcggcg agcgtgagcg ccacggtcgt
     5821 gccgagcaga ccgtgggcgg cggcacgcac cagcgactct tgatgctcgg cgccgctgct
     5881 cccggacgcc tcgatcctag gcttgccgag cgctccgaat cgcgccagtg tcgtgcgcgc
     5941 atcgtccaac cgtcctagat gttgcaggaa gcgcgcggat tcaggaatca acggactcaa
     6001 ggatatgaga gccagaccgg ttggcaaatt cagaagccac atgattcgcc agccgaagaa
     6061 tggctgcagc agcgcggaca gttcgctcgc ggcaaaatat ccgccgatcg tgcctatgcc
     6121 cccgaccagc accagacacc agccgcgatg ccgtgtgggc ataatttccg cgagcagtgc
     6181 ataggcgacc ggcaacatgc cgccggcggc cgctcccatc aggaagcaca tggcgatgtt
     6241 ccatgagaag gagggcatcg cgccgcagat cgaagtgccg acgaacatca ccgaggacaa
     6301 taatatcgac gcccgccgac cataaatgtc ggcgagtgct ccccacacaa aggagccgac
     6361 ggcggtgccg atcagcgcgg acagcggcaa taacgcaacg gtggtgcggt cgatgccata
     6421 ctcgacgcgc attcctggcg tgacaaagcc gaggcttgcc ggcttcatga cgtcgatgat
     6481 cagcgcaacg gccagtacca gcatcaggcg ccagtgggcg ctcgtcagag gcgcgtcctc
     6541 gggcggctcg aacggttcga gcacacccat gacaaccggg acgggcagca acccataacc
     6601 ggcggcaatg atgccgccga caatgcatgc catgcctgcc agcatgccgg gatccatggg
     6661 catcccggcc aggacgaagc catgtgcgtg cgccatccag aacatcggca gatggagcat
     6721 gaccccgatg ctgactatta gcgatcccac ccaaaacagc agcgcgctgc gccgctcgga
     6781 gaattggaat agcgcgtcga ttgcggcgtt gctctaactc tcgtagtggc gcggaaatag
     6841 gattcgaaag gtagtgccct ggcccttgac cgattccatc tcgatggtcg catccagcat
     6901 ttcgcacaag cgcttgacga tcgagagtcc gatcccttcg cccgcctcgc cgcgcaggaa
     6961 ggagcgcaat tcaccgtccg ggccgcttgc gaccgcagcg gctggtcgtg acgccaggcc
     7021 tgacgcgggc ggcgatgcgg cgggggttgc cgccggatcg agcgccgcgg ctagcggctg
     7081 gccagaggcg gtatgaaatc ccgggccggt gtccgagatg ctcaacatcc aacgtttgag
     7141 atcgcctgga ttgctatcac cccaggccac ggtgatgcag ccctcgcggg tatatttgac
     7201 ggcattcaag atcaaatttt gggcgatgcg gcggatcttc accgcatccc cgtcgatcgc
     7261 cagccctcgc ggtccctgcg cgagcaattg cagaccgcgc tgctgcgcca acgggcgaat
     7321 tccctcgcac aaggactcca acacgctggt cgcatcgagc ggctcgaccc ggcgcgactc
     7381 gcgtcccgcc tgcaggcgcg caagattcgt gacgtcatcg agcagttgat gaagcgagga
     7441 gacattgcgc atcaaaatgc gtatgaaatc ctctctcttg gcatcgcttg ggccatgccg
     7501 ggtaagcccc acggtgacat tggcgacgac gcccaagtta ccccgcaggt cgtgcgcggc
     7561 ttcttgccat acggccccgc gctcttgctc gagggcctgt acctgctcca gggccttctc
     7621 caagtccttg acgtgcccca cggcttcctg ctgctgcaag gtaaagtact gatgcacgct
     7681 cgtctcaatt ccggtgctca agaggatcgc ccacacttcc cgagcacggc tcatcgcatc
     7741 gagcgagatg ctcgagttct ccgtggcgta gcggtccaat tcgccgagca cgcactggtt
     7801 caacttgccc cattcgcggc tgacctcgcg caagtcatag ccctgctgcc agcgctgcag
     7861 cccgtgcgcg gcagcctgat cgtgcgcgtc cgcctcggcc ttgcgttccg ccgtgctcga
     7921 ggcgccgcgc aggcctattt cgaaggcacg caacagtgcc gggatgtgat caaataactc
     7981 ggagcgcggc agcgaatcgc cactggtgag gtccggatct ttcaggatcg cgcgacgcca
     8041 tgcgatcaag atcgcttcat gatgaatatt caaatgatcg gcgagcgccg ccagctcgtt
     8101 cggtaggtcg cgcactgtca gttgcccgtg aatttcggcg cgcgcttgtc gcggaacgcc
     8161 tgcacgcctt cggcaaaatc ccgggtgttg ccgagttcaa cttgggcgtc ggcttcgagc
     8221 gccagctgct cggacagcgt gcgcggcaat tccgtgttga aggcggcctt gatgcgccga
     8281 aacgcctggg tcggaccggc ggcgagacgg cgcgcaatgg cctgcgcttc ggcctgcagc
     8341 gccgtatcct cgacgcaccc ccagatgagt ccccaaccgg cggcatccgc ggccgacagc
     8401 gcatcgccca gcagcgcgag ccccttcgcc cgggccgtgc cgacgagccg cggcagatgg
     8461 aacgtgctgc cgaggtccgg catgagaccc aatttgggcg cgaacaattg caaaaatgtg
     8521 gcagagcgcg ccgcgagaac gatgtcgccg agcaatgcga gactgacacc ggctcccgcg
     8581 gcgaccccat tgacggcaac gaccaccggc acgcgcagct ggtaccaggc gttcaccatg
     8641 ggattgaagt gttcgcgcag ctcgatcccg atgacctcgc cgaggcgcgc ctcggcgcca
     8701 cccgagaaat tactgcccat gaggtcggcg cccgaacaaa agccgcgtcc cgcagcgccg
     8761 aggatcacgg cgcggatctc ggcgtcggcc tcaatggccg cggccgccgc ccgcaaatcg
     8821 gcgatcaacg ccatattcag ggcattgagc cgatccggcc gattcagagt gacatgggcg
     8881 atgccgtctc gcctctcgaa aagcaaagtg tcaaattgca tgatgatccc gggtgcggcg
     8941 cagcggagta cgccataatg acagaaaccg gtccatgtta aatactgcgg agtgatacat
     9001 gccaattcct gagattctgc agggtcgcct gcggatgccg gtcatcggcg cgcccatgtt
     9061 catcgtctcg acgccgcggc tggtgcttgc tcaatgcaag gcgggcatcg tcggcgcgtt
     9121 cccgtcgctc aacgcgcggc ctgcggcgca gctcgacgac tggctggcgc aaataaccag
     9181 cgaactcgcg gattatcgcc gcgccaaccc gcatcttaag gtggcgccgt tcgccgtcaa
     9241 tcagatcgtg catagcagca acgatcgtct cgaacacgat gtagcgctgt gcgtgaagca
     9301 tcgggtgccc atcatcatca ccagcttgcg gccgccctcc gaagtggtgg cggcggtgca
     9361 cggctacggc ggcgtggtgt tccatgacat catcaacctg cgccatgccg aaaaagccgc
     9421 ggcgcaggga gtcgacggca tcatcgccgt gtgcgccggc gcgggtggcc atgccggcgt
     9481 cttaagtccg ttcgccttgg tgaaacagat ccgcgaagtg tacgccggta ccatcattct
     9541 ctcgggcgcg atgagcagcg gcgcggacgt gttggcggcg ctcgcgttgg gcgcggatct
     9601 cgcctatctc ggcacgcgtt tcatcgcgac ggaggaggcg aatgcccccg ccgagtacaa
     9661 gcagatgctg atcgagagcc gcggcgagga catcgtgtac acctcgctgt tcagcggtgt
     9721 gttgggcaat tacctgcgcg gcagcgtcac gcgcgccggg ctcgatccgg acaagcttcc
     9781 cgaagcggat aaagccaaga tgaatttcgg cagcggcggc gccagcgaat tgaaggcgtg
     9841 gcgtgatatc tggagtgcgg gacaaagcgt cagcggtatt cacgatgtca aggcggtcgg
     9901 cgccttgatc gagggcatgg cgcacgagtt caacgcggcg cgcgcgcggg tcaatgcagt
     9961 gcagattgcg gatgggccgg tataacgtgg ccgcatgcgt ttaagcgccg accaattatc
    10021 cgccgcgctg tcgcggggcg tggcggcgcc catttacctc gtttccggcg atgaaccctt
    10081 gctggtcggc gaggcggcgg acgcgattcg cgccgccgca agaaccgcgg ggtacgcgga
    10141 tcgcacggtg ttcttcatcg atcgcagttt cagctgggat gagttcaagc acgcgaccca
    10201 ggccttgtcg ctgttcgccg agcggcgcct gttcgagctg cggatgccaa gcggcaagcc
    10261 ggacaagggt gcgcagctgc tggcagaagt cgccacgaac ccgccgcccg atgtcgtgtg
    10321 cctggttctg tgcgacaaac ttgacaagaa ggcgagcgag gctccgtggg ttcgcgccgt
    10381 cgagaagcac ggcgtgtggg tccccgtttg gccggtgcag agcgccgcgt tgccggcctg
    10441 gctgcgcgag cgcgcccgac aactgcacgt gcagatcgag cctgccgcgg cgcagctcat
    10501 cgtcgagcgg gtggagggaa atttactcgc cgcgaaacaa gagctcgaca agctatgcat
    10561 gctggccaac ggcgcaccga tcgatgccga gttggtgttg cgctcggtgg gcgatagcgc
    10621 tcgctacgat gtgttccagc tggcggccgc ggccgccgcc ggcgaggcag cccgcgcact
    10681 gcgcgtgctg ctgggactga agagcgaagg cgtggagccc acgctgatat tatgggcgct
    10741 ggtgcgcgag ctgcgaggcc tgtggcaggc gcgggaacgc gaccgcttgc gctcgacggt
    10801 tcgcggcagc ggctggaatc tcgcggccac gccgtcgccg cgcgcactgt cgcggctgaa
    10861 gcagctgccg ctggagcagc tcatgaagca ggccagccac gctgatcggg tgatcaaggg
    10921 actgtcggcg ggcgaaccgt ggagcgctct gaccgggctc gcggggggcc tcgccggcgc
    10981 tttgcaagcg acagcagatt caggcagggt acggctatga acccgatggg tattttcggc
    11041 ggtactttcg atccgattca ttatgcgcac ctgcgcacgg cgttcgagct gcaacaggcg
    11101 ctgcgactca aagagattcg ttttctccct gccggcaatc cgccgcaccg cgagcggccc
    11161 attgccgatg ccgagttgcg gctgcagatg gtgcaactgg caaccgccgg gcaaagcggt
    11221 ttcgtggtcg acgaccgcga ggtgcgcaag caaggtccct cttactcggt cgagacgctg
    11281 gcggaactgc ggcacgagta tccggaccgt agcctgtgtc tgatcgtcgg catggatgcg
    11341 ttcttgagct tgccaaaatg gcatcaatgg cgcgacctgt tgcagctcgc ccatttggtg
    11401 gttgcgcatc gccctggttg gcgtgccccg agcatggggc cgctcggcga gctcctggtc
    11461 gacagcggca ccggccggat cggcgatctg cacgatacac gcgcgggatg catctacatt
    11521 catgcggtga cgcagctgga gatttcctcg accgaggttc ggcagctgat cagcatgggc
    11581 cgtgatccgc gctttctgat gcccgacgcg gtgcgtaaac tcatcctcga tacccactgt
    11641 tattccaaaa cccggacaac gtagtgccag tcaaaaaaaa gtcatcaaat ggtaaaataa
    11701 agggccgcaa gaaagcgctc cgccactctc cagccggcaa gtccaaacct gccggcgaca
    11761 tcaaagctgc cagacacccc gcgaccgccg ccgcgcgcaa gccggccaag gcagggccgt
    11821 cgctgaagag cgtggtgatc gatgcgctcg acgacatgaa ggcattggaa gtcaaggtgc
    11881 tggatgttcg cggcctcacc gacgtcgcgg actacatggt gatcgccagc ggcacctccg
    11941 accgccacgt ccgctccgtc gcacaacgcg tcgtcgagcg caccaaggag gcgggcttcc
    12001 gtccgcatgg ggtcgagggc cagcaagaca gcgattgggt gctcatcgat ttgaacgaga
    12061 tgatcgtgca cgtcatgctg ccgcgtgtgc gcgagttcta tggtttggaa aagctgtggg
    12121 atatcacggc gaccaagcgt gcggcgcgcg cctagcggca ggcccacccg atgaaatgcc
    12181 ggttgatcgc tgccggtacc cgtttgcccg aatgggtcaa tggcggtttt cgcgaatacc
    12241 agaagcgctt gcgtacgccc ttcgtgttgg agctgcacga gattgcggtc gcgacgcgac
    12301 gtgccgggga aaatccgcag cgtgcgattc aacgcgaaag cgacgacatg ctcgccgcac
    12361 tcaagcagaa cgattacgtg gtggcgctcg agatcgcggc gccatccatg agcaccgaac
    12421 aattgagcgc ctggctcgcc gagcgcatgc gggacgggcg ctcgcttgcg ttgctcatcg
    12481 gcggtcccga tggcctatcg gcgcaatgtc gcgagcgggc cgaccgaagc tggtccttgt
    12541 cgccgctcac cctgccgcat gccctggtgc gggtggtggt ggccgaacag ctctaccgcg
    12601 ccatgagctt gctcgccggt cacccgtatc accgagctta agggcctgcc gttcggtatc
    12661 ctggcgtcat gcactcagat ttcgtctact tagcctcggc gtcgccgcga cggcgcgagc
    12721 tgctgcaaca aatcggcgtg gcgttccgca cggtcgggtc gaacgtcgac gaaacggcgc
    12781 tgccgggcga ggcgccgctc gattatgtca cgcggctcgc ggcggccaag gccgaggccg
    12841 gttggcagca gagccgcgac ccggcgcagg ccccggtctt ggcggcggat acggcggtcg
    12901 tcctcgacgg cagcatactc ggcaagccca aagatcggca ggatgccgag gccatgttgg
    12961 cgcggctctc cggccgcacc cacgaggtca tgaccgcggt ggcgctgcgc accgcgcagg
    13021 gtcttcgcgc caaggtttgc cgtagcgagg tgacctttcg gcccatcgcg gccgccgagg
    13081 cgcgtgacta ttgggaaacc ggtgaatccc aagacaaggc cggcgcctat gccattcaag
    13141 gccgcgccgc ggtgttcatc gcggatttgc gcggcagcta ttccggggtc atggggctgc
    13201 cgctgtacga gacggccgaa ctcctgagtc aagccggggt tacctggcat ggccgcgaag
    13261 cgcggtcatg agcacggaaa ttctggtcaa cgccagcact cacgaagccc gtgccgcgct
    13321 ggtcgagaac ggcgtgctgc aagaggtgta tctcgagcgc gccagccgtc gcggattgat
    13381 cagcaacatc tacaaaggac gtgtatcgcg ggtgctgccc gggatgcagg cggctttcat
    13441 cgagatcggc atggagcgca cggcgttctt gcatgcctcc gacatcttcg atgcgcgtca
    13501 tgtggacacc ggcatcgagc aaccgcgcac ggagaatatc cgggcgctgg tggccgaggg
    13561 caacgaaatc ctggtgcaag tggtcaagga tcccatgggc accaagggcg cgcggctgac
    13621 cacttacatc actttgccgt cccgctatct tgtgtacatg ccgaagggtc atggagtcgg
    13681 cgtctcggct cgaatcgaga gcgagggcga gcgcgagcgc ctgcgtgctg ctgtgcaggc
    13741 cgcgaccgaa tccggcgagc aagccggctt catcgtgcgc acggctgcgg aggatgcgcc
    13801 gcaggaggcg ctgcgcgccg acatgctgta cttgcgcaag ctctgggaat tcgtgcgtca
    13861 caatggcctg cgcacgcagc ccggtaatct ggtgcacgcc gacttgccgc tgcacctgcg
    13921 cgtgttgcgc gatttgctgc gtccggaagt ggacagggtg ctcatcgatc aagcgaacgc
    13981 tcaccgcgaa atgcgcgagt tcgccgccgc cttcatgccg caggcgctgc aacgtctcga
    14041 attatacgat ggcgtacgcc cgctgttcga gctgcaccac gtcgaggagg aaatccagaa
    14101 ggcgctggag cgcaaggtgt cgctgaaatc gggcggccat ctgatcatcg atcagaccga
    14161 agccatgacg acgatcgacg tcaataccgg cgccttcgtc ggccatcgca acctcgagga
    14221 aaccattttc cgcaccaatc tcgaggcggc ggtgaccatc gcgcgccagc tcaggctgcg
    14281 taatctcggc ggcatcatca tcatcgattt catcgacatg gaagagccgg agcatcgccg
    14341 tcaagtcatc caggcgctcg aaagggcgct cggcgacgac cacgtgaaga ccagcatctc
    14401 atcggtgtcg cccttggggc tggtagaaat gacgcgcaag cgcacgcgcg aaagcttgga
    14461 acatatgctg tgccggcctt gtccgacttg cgagggcaaa ggcttcgtca aaacggcgga
    14521 gacggtgtgt tatgaggtat tccgtgagat cgtgcggcag tcgcgccagt tcgagtgtca
    14581 acagttgatg gtgctggcgc atcaagatgt catcgaacgg ctgctggacg aggagtcctc
    14641 ggcgctcggc gaattggaac tggtcaccgg caagcccatc cgcctgcaaa ccgaagcgct
    14701 gtatagcgtc gatcagtacg acgtcgtcct gatgtaaatg agcgcacgca aggtcggaaa
    14761 aatcctgctt accggtttcg caggactgct cagcctgttg ttgctcgcgc tgttggccgc
    14821 caagctgatg ctcgatcgcg cgcccgccta cgaaggcgag atcaagcaat gggtgttcgc
    14881 gcagaccggg tatcacatcg attttgccaa tgtttcgccg tcgtttcgct ggtatggccc
    14941 ggaattgcac ttcgagcacc tggaactgcg ctccaaggac gatcggcggg tgctggcgcg
    15001 cgccgcgggc ggccgggtcg ccgccgacat ctggcagctg ctgcgcagcg gcaagctgct
    15061 cgccggtagg atccagctcg atgcgccgaa cctcgtcatt gcgcgcttgg ggccggcaag
    15121 ctttgcgctc gcctccgaga tcaaattggg tggcgacaac tcctcgctcg aggccctgac
    15181 gctcaacgac ctgccggtcg gcaagctcgt gatccgcgat gccacgatca cgctgcagaa
    15241 ctggaacggc gcattgccca agctcgaatt acgcaaggtc aatcttgacg tgcggcgcgg
    15301 tgaccagggc cttgcgctct gggtcgacgc acggttgccg gaggcgctcg gcggcacctt
    15361 gaatttcgcc ggcaatgcgc gcggccacgg cgagtttcaa atgctgcgat ggagtgcact
    15421 ggctcgggca cgcgatatct catttccggg atggcgctcg ctgctgccgg aatacctgag
    15481 ccggctcgat gcgggcgatg gtgcgttcga cctggcggcg agtggggtcg gagcgatgct
    15541 ggagcgcgcg gacctcgatt ttgccgcgac aggcgtggtc acccagctgc aggaaggacc
    15601 gagcgcaaag ttcgagcaga tcagcggcgc gctcagcatc gtacactcga gcgatcgctg
    15661 gacgctgacg ggcaggcggg tacgcgcagt gcgccaaggg cgccgcgatc cggactcgga
    15721 gttcaatgtc agttggcgcg gcggcgattc gggattattg gaactgcgcg ccgctgcgag
    15781 ctatttgcgt gccgatgccc tgctgccgct cgcgggactg cttcccgaga aggagattcg
    15841 cgagcggctg caggaaatcg cgcccaccgg cgagtggctc gacagcttcg tggcgctcgc
    15901 gcgcaattcg gcacaagacc cgtggcgcct gcaggttcgg gcgagatttc acgaagtcgg
    15961 ttttgcgccg gtgggccggg cgccgggcct gcggggcttg agcggatcca tcgccggcaa
    16021 tgagaacggc gggcatgtga aaatcgaacg tggcgcggcg ctctttacct ggccggcgca
    16081 gttgctcaag ccggtcggac tcgattcgtt ggcagcgacc ttgtattgga agcgcacccc
    16141 cgatgcgttg ttggtcgcca gcagcgattg gaccatggcg aatcgcgatg ccggcgctca
    16201 cggcaaggtg gcgtggcagc agccggccga cggctcatcg cctctgctga cgctggcggg
    16261 cgctgtggaa aacggccatg ccgcggaaac gcgcagttac tttccgcgcg cgttgatcc
//
LOCUS       DASWZT010000198         4221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_40332, whole genome shotgun sequence.
ACCESSION   DASWZT010000198 DASWZT010000000
VERSION     DASWZT010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40332"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VGE92_05345"
     CDS             <1..586
                     /locus_tag="VGE92_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEY1313278.1"
                     /translation="TPNLVLSREETRRRLDFAVTQEPVTAVQLSRGKAEAQLLFEITL
                     LAQDLGRLAADLLLYYTQEFSFVSLPAAFTTGSSIMPQKRNPDVFELVRGRSCVAQAA
                     LNEVLAVTQKLTSGYHRDLQLIKPPLFRGIDSCLQTLKILPSALDGVSFRPEHIRLDP
                     GIHAAAAANALVAKEGIPFREAYRRVAGTLKDSE"
     gene            583..1020
                     /locus_tag="VGE92_05350"
     CDS             583..1020
                     /locus_tag="VGE92_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013359891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HEY1313279.1"
                     /translation="MTIWRSLRSLADLNSGGSGTLVEHLGIEFSEIGADFVRATMPVD
                     HRTRQPYGLLHGGASVALAETVASTGATMCVDAAEYLCVGQEINANHVRSARSGRVTA
                     TARPVHLGGRTHVWTIDIVNDDGKLVCTSRLTVAIVRRGTLGG"
     gene            1065..1862
                     /locus_tag="VGE92_05355"
     CDS             1065..1862
                     /locus_tag="VGE92_05355"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY1313280.1"
                     /translation="MTHTRTAFLRDFCMAGLLLAAAAPLGAQTPAPGTNPPPAAAAAG
                     ANPTAPSADDIGYLFGLSFGEQMHNVGITDQLSPEALSRGLKDGMQGKKTTPADRAQV
                     QGYIRSVMDAAVARNEAQAKDFLARNGREKGVTTTASGLQYRILLPGDRKAAAITPTD
                     GVTVQYRGKLIDGTQFDSTYDRNAPASFAVNGVIPGWQEALLLMKPGAKWQLFVPPEL
                     GYGKSPKPGIPAGSLLIFEVELLSVKSSSGPPVPPGTPANPPAPRSK"
     gene            complement(1878..3575)
                     /gene="acpA"
                     /locus_tag="VGE92_05360"
     CDS             complement(1878..3575)
                     /gene="acpA"
                     /locus_tag="VGE92_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase"
                     /protein_id="HEY1313281.1"
                     /translation="MNRMLRTRGAARLLLALGMGAAVLGGCAHGDRLASSDSNVAVEA
                     ALRAKVETIVVIYAENRAFDNLYGNFSGAHGLSEVIDGNGRPLPSYVPQIDRNGSVLA
                     ILPPTWGGVTAPGTQPVVTEAQSAGLPNAPFSIEHAFTPQANVTLSTSTVTRDLVHRF
                     FEHQMQIDGGKNDGYAAWSDAGGLAMGHYDYSQSALYSLAREFVLADDFFQGAFGGSF
                     LNHQYLVCACAPRYPGADSAAAKPSIALLDQDAAGHYLPRLTLARDARGSALDAPPSF
                     ARNGNITPLNYFGDGEFYAVNTMQPAYQPSGNPPAAGDADFLFADPMKATTLPPQTQA
                     TIGDRLDAKHIRWVWYAGAWIAASADGRQPPQKTRTAIYAPLYAGADPDFQAHHQPFN
                     YYERFDPAAHAEYRAVHLQDYSALLADAAAGRLPAVAFYKPQGNLNQHAGYASVAEGD
                     AHIADLVAKLRASPQWPHMVIVITYDEFGGAWDHVAPPHGDLLGPGARIPALIISPFA
                     KRGTVDHTQYDTASILRLITRRFDLAPLPGVTLRDQSLAAHGAPPMGDLTNALDLGR"
     gene            complement(3674..>4221)
                     /locus_tag="VGE92_05365"
     CDS             complement(3674..>4221)
                     /locus_tag="VGE92_05365"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY1313282.1"
                     /translation="VNPVGFYIGAGVGYSTIRSDDPAYGLPGYFNDHQTAWKAIVGVR
                     PIPIVGAELEYIDFGQPGQHNGYYNSAYYGLDSHPRAGALFAVGYLPIPVPFLDIFGK
                     AGVARLETDVTSTSSNCAPGTTCSAEATIFRTNRTDNKFTYGVGVQSRAWGVAFRAEY
                     ERISSTYGDPDALTVSATWTF"
BASE COUNT          764 a         1306 c         1407 g          744 t
ORIGIN      
        1 cacgccgaac ctcgttctga gtcgtgaaga aacgcgccgc cgtctcgatt ttgcggtcac
       61 tcaggaaccc gtgacggcgg tgcagctgtc gcgtggcaag gccgaagcgc agttgctgtt
      121 cgagatcacc ctgctggcgc aggacctggg acgcctcgcg gccgacttgc tgctctacta
      181 cacccaggaa ttcagcttcg tgtctctgcc ggcagctttc accaccggct cttcgatcat
      241 gccgcagaag cgcaaccccg acgtgtttga gctggtgcgc ggcaggagtt gcgtggcgca
      301 agcggcgctc aatgaagtgc tcgcagtgac gcaaaaactc acctcgggtt atcaccgcga
      361 tctgcagttg atcaagccgc ccttgtttcg cggcatcgat tcctgcttgc agacgctcaa
      421 gattctgcca agcgctctcg atggcgtgag ttttcgcccg gaacacatcc ggctcgaccc
      481 cggcatccac gccgccgccg ccgccaacgc cttggtggcc aaggagggta ttccgtttcg
      541 cgaagcttac cgccgggttg cgggcacgct caaggattcc gaatgacgat atggcgcagt
      601 ttgcgctcgt tggcggattt gaattcgggc ggcagcggca cgctggtcga gcatctcggc
      661 atcgagttca gcgaaatcgg cgctgatttc gtgcgcgcga ccatgccggt cgaccaccgc
      721 acgcggcaac cctatgggct gctgcacggc ggcgcatcgg tggcgctcgc cgagaccgtc
      781 gccagcacgg gcgcgaccat gtgcgtcgac gccgcggagt atttgtgcgt cgggcaggaa
      841 atcaacgcga accacgtgcg ctcggcgcgc tccgggcggg tgacggccac ggcgcgtccg
      901 gtgcacttgg gggggcgcac tcatgtgtgg accatcgaca tcgtcaatga cgacggcaaa
      961 ttggtgtgta cgtcgcgcct gaccgtggcc atcgttcggc gcggcacctt gggcggctaa
     1021 tttcgcgctg gcgcagctcg cccgcaggag cgtatcttcg cgtgatgaca catacgagaa
     1081 ctgcatttct tcgcgacttc tgcatggccg ggctgctgtt ggcggccgcg gcgccgctcg
     1141 gcgctcaaac gcccgctccc ggaacgaacc cgccgccagc ggcggctgcc gctggcgcga
     1201 atcccaccgc accgagcgcc gatgacatcg gctacttgtt tgggctgagc ttcggcgaac
     1261 aaatgcacaa cgtcggaatc accgaccagt tgagccccga ggcgctctcg cgcggcttga
     1321 aagacggcat gcaaggcaaa aaaaccacgc cggcggaccg cgcgcaggtg caggggtaca
     1381 tacgttcggt gatggacgca gcggtggcgc gcaatgaggc tcaggcgaag gatttcctcg
     1441 cacgcaacgg ccgcgagaaa ggcgtgacga ccaccgcctc cggcttgcag tacaggatct
     1501 tgctgccggg cgaccgcaaa gccgccgcga tcacgcccac cgacggggtg acggtgcagt
     1561 atcgcggcaa gctcatcgat ggcacgcaat tcgacagcac gtacgatcgc aacgcacccg
     1621 catcgttcgc cgtgaacggt gtgatccctg ggtggcaaga agcgttgttg ctgatgaagc
     1681 cgggcgccaa gtggcaactg ttcgtgccgc cggagctcgg ctatggcaag agccccaagc
     1741 cggggattcc ggccggttcg ctgttgatat tcgaggtgga attgctcagc gtgaaatcga
     1801 gcagcggccc gccggtgcct ccggggactc cggccaatcc gccggcgccg cgctccaagt
     1861 aaagcggcgc cgctgggcta gcggcccaaa tcgagcgcgt tggtcagatc gcccatcggc
     1921 ggggcaccgt gcgcggcgag gctttgatca cgcagggtga cgcccggcaa tggggcgaga
     1981 tcgaagcgtc gcgtgatcaa tcgcagaatc gacgcggtgt cgtactgcgt gtgatcgacc
     2041 gtgccgcgtt tcgcgaatgg cgaaatgatg agcgcgggaa ttctggcgcc cgggcccaac
     2101 agatcgccgt gcggcggcgc cacgtggtcc cacgcgccgc cgaattcgtc gtaggtaatt
     2161 acgatcacca tgtgcggcca ctgcgggctt gcgcgcaatt tcgccacgag atcggcaatg
     2221 tgtgcatcgc cttccgcgac ggacgcatag ccggcgtgct ggttcaggtt gccttgcggc
     2281 ttgtagaaag cgaccgccgg caagcggccc gcagccgcgt cggcaagcag cgcgctgtaa
     2341 tcttgcaggt gaaccgcgcg gtattcggcg tgcgccgccg gatcgaagcg ttcgtagtag
     2401 ttgaagggct ggtgatgcgc ctggaagtcc ggatcggcgc ccgcgtacag cggcgcataa
     2461 atcgccgtgc gggtcttctg tgggggctga cggccatccg cgctcgcggc aatccacgcg
     2521 ccggcatacc agacccagcg gatatgtttc gcgtcgagcc tatcgccgat cgtcgcctgg
     2581 gtctgcggcg gcaaggtagt ggccttcatg ggatcggcga ataaaaaatc cgcatcgccc
     2641 gctgccggtg ggttgccgct cggttgatac gcaggctgca ttgtgttgac ggcgtagaac
     2701 tcgccgtcgc cgaagtagtt gagcggcgtg atattgccgt ttcttgcaaa gctcgggggg
     2761 gcatccaagg ccgagccgcg ggcgtcgcgt gcgagtgtca gacgcggcaa gtaatgtccg
     2821 gcggcgtcct gatcgagcaa cgcgatcgag ggcttcgctg cagcgctgtc cgcgccagga
     2881 tagcgcggtg cacaggcgca gaccaggtac tggtgattca gaaatgaacc gccgaacgcg
     2941 ccttggaaaa aatcatcggc gagcacgaat tcccgggcga gcgaatacag cgctgattgg
     3001 ctgtaatcgt aatgacccat cgcgagaccg ccggcgtcgg accacgcggc gtagccgtcg
     3061 ttcttgccgc catctatctg catctggtgc tcgaaaaagc ggtgcactaa atcgcgggtt
     3121 accgtggacg tcgacagtgt cacgttggct tgcggcgtga aggcgtgctc gatcgagaac
     3181 ggcgcattcg gcaagccggc gctctgtgcc tcggtcacga ccggttgcgt gcccggtgcg
     3241 gtgactccgc cccaggtcgg cggcagaatc gcaagcaccg agccattgcg atcgatctgc
     3301 gggacgtagg aaggtagcgg ccgtccgttg ccgtcgatca cctcggagag gccgtgcgcg
     3361 ccggaaaagt tgccgtacaa attgtcgaag gcgcgatttt ccgcgtaaat gaccacgatg
     3421 gtttcgacct tcgcgcgcag cgccgcttcg acggcgacgt tgctgtcgga gctcgcgagg
     3481 cgatcgccat gcgcacatcc gccgagaacg gcggccccca tcccgagcgc aagcagcaag
     3541 cgtgcagcac cgcgcgttcg aagcatacga ttcatgcgcg caagtatagg cggccgcacg
     3601 cagcggcgcg acccagccgc gcgcggctac gctcgggaaa tcgagccggg cttgatgcga
     3661 aagcggcgca tgttcaaaac gtccaggtcg cgctgacggt cagcgcgtcc ggatcgccat
     3721 acgtagagct gatgcgctcg tattcggcgc gaaatgcaac tccccaggct cgcgactgca
     3781 cgcccacgcc gtaagtgaat ttattgtcgg tgcgattggt gcggaagatc gttgcctccg
     3841 ccgagcaggt ggtacccggc gcacagttgc tcgacgtgct ggtgacatcg gtttccaagc
     3901 gcgccacacc cgctttgccg aagatgtcca ggaacggcac cggaatgggc aaatagccga
     3961 cggcgaacag ggcgccggcg cgcggatgag agtcgagtcc gtaataggcg ctgttgtagt
     4021 agccgttgtg ttgaccgggc tgcccgaagt cgatgtactc gagttccgcg cctacgatgg
     4081 ggatgggacg cacgcccacg atcgccttcc acgcagtctg gtggtcattg aaatagccgg
     4141 gcagaccgta cgcgggatcg tcgctcctga tggtggaata gcccacgccg gcgccgatat
     4201 aaaagccgac cggattcacg t
//
LOCUS       DASWZT010000199         1648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_45051, whole genome shotgun sequence.
ACCESSION   DASWZT010000199 DASWZT010000000
VERSION     DASWZT010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45051"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1084)
                     /locus_tag="VGE92_05370"
     CDS             complement(161..1084)
                     /locus_tag="VGE92_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313283.1"
                     /translation="MSGRAKAWTILSHRLTKAADWPRIPALDDTAITRLIRNYQFANE
                     RRSTTRYTADITYVFNSDAVRHMFRTANIAYADMQAKPILVVPMSPGYVPHSAWTSIW
                     ANPKYATGAAPLVLPVGDALDGAALSVLNFNTATWQDVEPVASRAHAAEAFLVQVNQG
                     KGSIDIKLRRLGPGNSPPMPDVVMPIPPGVTGQKVYASAADAAASAIIDEWKARSAID
                     FGKRSKLIVEVHIQDLAGWSALLQRLGTIPNITDVGVVAINTGEARVALTYVGTQDQL
                     HDLLAQATLDLSNQNGAWWLGPLTPADASTQ"
     gene            complement(1295..>1648)
                     /locus_tag="VGE92_05375"
     CDS             complement(1295..>1648)
                     /locus_tag="VGE92_05375"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY1313284.1"
                     /translation="DAFKVSLGPTWDKTAIVMATEFGRTVKPNGNGGTDHGTAGAAFL
                     LGGAVQGGVVKAEWVGLEPAALQDGRDQPPRTDQRALFKAALADHMGVSRRDLEGSVF
                     PDSGAITPLSGLFRA"
BASE COUNT          314 a          515 c          525 g          294 t
ORIGIN      
        1 cgcttggcga ggaagccgcc ggccgcatcg ctcaatccgg caaacgcaaa tatgccgagt
       61 gcggcacggt cgtcaccgct tgcgagtagc atggcgagcg cgggcgcgca ggcgagccgc
      121 attccggtca gcaggttcgg aacatgtttc agaaaaccgc tcactgcgtg ctcgcgtcgg
      181 cgggtgtcag cgggccgagc caccaggctc cattctggtt cgaaagatcg agtgtcgcct
      241 gtgcaagcag atcgtgcagc tgatcctgcg ttcccacata ggtcagggca acgcgcgctt
      301 cgccggtgtt tattgcaaca acaccaacat cggtgatgtt ggggattgtc cccagcctct
      361 gcagcagagc cgaccaaccg gccagatcct ggatgtgcac ttcgacgatc agtttgctgc
      421 gcttgccgaa atcgatggcc gaacgcgctt tccattcgtc gatgatggcg ctcgcggcag
      481 catcggcggc gctcgcatag actttctgcc ccgtcactcc aggcggaatg ggcatgacca
      541 cgtccggcat cggtggcgaa ttgccgggcc cgagccgcct cagcttgata tcgatagatc
      601 ctttgccctg attgacctga acgaggaacg cttcggcggc gtgcgcccgc gacgcaaccg
      661 gctcgacatc ctgccacgtc gccgtattga aattaagcac ggacagggct gcaccatcga
      721 gcgcatcacc cacgggcaat acaagaggtg cggcacccgt cgcatatttc ggattggccc
      781 agatggacgt ccaggccgag tgcggcacat agcccggcga catcggcacg accaggatcg
      841 gttttgcctg catgtcggcg taggcgatgt tggcggtgcg gaacatgtgc cgtaccgcat
      901 ccgaattgaa cacataggtg atgtcggcgg tgtagcgggt ggttgaacgt cgctcgttgg
      961 cgaactgata gttccggatc aggcgggtga tcgcggtgtc gtccagcgct ggaatgcgcg
     1021 gccagtccgc cgctttggtc agacggtgcg acagaatcgt ccacgccttt gcacgccccg
     1081 acacgatggc gatgttctgc gcctgggtcg ccgaagaggc agtcgcgtcg accgcgatgc
     1141 cgctgacggc atacggatct cccgcggcct gtgcgaccga gagcggagca aaaatagcaa
     1201 gtccaaccag caagaaaaag gtgatgcgaa gcatgagggc gcccgtcgag gtcgccggac
     1261 tatactgatc cggccgcgga ggcgaaagcg cggctcaggc gcgaaacagc ccactcaatg
     1321 gggtgatcgc cccgctatcc gggaaaaccg agccttcaag atcgcggcgc gagactccca
     1381 tatggtcggc aagcgccgcc ttgaagagcg cacgctggtc cgtgcgcggc ggctggtcgc
     1441 gcccgtcctg aagtgctgca ggttcgagac ccacccattc ggctttgacg acgccgccct
     1501 ggaccgcacc gcccaggagg aaagccgcgc ccgcggttcc gtgatccgtc cccccattgc
     1561 cattgggctt cacggtgcgg ccgaattccg tcgccatgac gatggcggtc ttatcccaag
     1621 ttggtccaag tgacaccttg aacgcgtc
//
LOCUS       DASWZT010000200         5386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_54771, whole genome shotgun sequence.
ACCESSION   DASWZT010000200 DASWZT010000000
VERSION     DASWZT010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5386
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54771"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..253)
                     /locus_tag="VGE92_05380"
     CDS             complement(95..253)
                     /locus_tag="VGE92_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017129692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HEY1313285.1"
                     /translation="MSLGTILLIVLLLLLVGALPAWPYSGGWGYYPSGGLGLVLIVLL
                     VLVVTGRL"
     gene            complement(348..1310)
                     /locus_tag="VGE92_05385"
     CDS             complement(348..1310)
                     /locus_tag="VGE92_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313286.1"
                     /translation="MDFVSAAAAPQVARHAASVPRLGVWVPWADTDSIGWVRYSLDQR
                     HIPYVYIRDEDIRAGHLQGKIDVLLYGHVDLELAEQIQGIPKAWGPMPFKKTAQTPSH
                     GTPAASDDITGGIGWQGLAQIQQYIEHGGLLVTLGNGTMLPIEGGIVRGVRRDSGGVP
                     RSTAGGGASSSEASRESVTRTPGAHVRVSFVRPDHPIAYGYTPSTYVFRSNFALYSVP
                     RRWLRMAYCQTCLDGPFDPRGVVLQWGDPQNPAPFLVSGQVWGEDNLLGRPAIFDLPV
                     GQGHVVAFNFNPLYRDSNRGDQRMLWNVILNWQAILAPKGAPAR"
     gene            complement(1314..3173)
                     /locus_tag="VGE92_05390"
     CDS             complement(1314..3173)
                     /locus_tag="VGE92_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEY1313287.1"
                     /translation="MRKWLIAILCAAWAASVDVSAQGLIAPDSAEPGSVEEIAKATTE
                     TRFLSPWVAYLPASAAVPSPRAFFGRISGAPGELVDSAQAYAYSRALAAASPRVRVFT
                     IGRSEEGRDIILLAIADEKGIQQLDAMKSANASLADPRTTDGADAERLIAGSRPFYYF
                     NAALHSDETGSTESVLELGYRLAVSEQPMIRRIRETMVVLINPVCNPDGRDKQVEWFY
                     RFLKGKTDRDSLPRQSPPYWSKYAFVDINRDAHQLAHQTSKAVSRMFFDYHPVIVHDL
                     HESVPLLMSWNGTGPYNPNIDPITFTEFLALSFHEVETLTGLGMPGVSTWNFGEGFGH
                     MFTDSVAMNHNAIGRGYETFGNGTAETLTQSLSPEETSPQWYRVVPPPSGSFSWSARD
                     NVNYNETAALAALDYAAANSRMLLRNFYTKSLHSLRRGLDAPPYAFIIPAEQGDPARV
                     AQLVARLQAQHIEVSRTDVPLMLTEGTFPAGTYVVKLGQPYRNYAVDLLTPQHYPKDA
                     GEPYDDVSWELPAYFHLEVIATADRAIRDMPLALLTVPPQPHGRLLGAGPVYLLKDSG
                     QEGLLEARYRLARFHVEIAEHAFVVDNVSYPAGSWIVAAQNGVAAALRDTADQ"
     gene            complement(3158..4591)
                     /locus_tag="VGE92_05395"
     CDS             complement(3158..4591)
                     /locus_tag="VGE92_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY1313288.1"
                     /translation="MSAASTASAVRGELTRGVGPWAATAVNVANMIGTGVFLKTRVMT
                     CNVGSAKLVLAVWLAAGLLSLAGTFSYSEIAAMMPEAGGDYVYLRRAYGRLVGFLYGW
                     ICFTVARAGSQAALAVGLAIFMNVALGGALDRWHAAGTLGPHLNGLTMVALAAIWIVA
                     LINCASVAAGGRMALALTIAKVGLVLGVGVGAFVFAAGDWGHLTQSGISGTCAGVAQS
                     ARGGIAGFGAAMLGALWAYDGWQNVAPLAGEISNPQRNLPRAFVGGTLIVAALYLFVN
                     IAYYYALTPVAIASVAVNSSVATEVLKKFLGPLAVSMTAVALMVSSFGALHASVLANS
                     RIPFAMARDGLFFRSLTRLSPRSNVPVRAILAQAAWASLLALSGSYDALTDSVIFSSW
                     LFYGLVTASLFIFRRKLPDAARPYRAFGYPVVPMIFLLATAALLINTFVAAPHEAFRG
                     VMLLAAGVPFYWYWSRRPSSEVSCENG"
     gene            4590..5159
                     /locus_tag="VGE92_05400"
     CDS             4590..5159
                     /locus_tag="VGE92_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313289.1"
                     /translation="MSASIEQMQPDFSQFGPLLLAALVVFAVYRRFRRSFGRQLLSPK
                     RMTFRIVLLAVIGCALLPLALRSTQYLGAELLGAALGAALGWWGAERTRFVRDAGRLF
                     YVPHTYTGIAVSLLFLGRLVFRLLQAYGAHAQASQALSTPAMVRSPLTVGIFFVLLGY
                     YLYFYSWVLWKSNRLDVSDIEAASPAAAQ"
BASE COUNT         1082 a         1702 c         1673 g          929 t
ORIGIN      
        1 ccgcctcgag tttcgatttc tccgcaaggc aagtcgtgaa cacgagcacc gacagcagca
       61 cggttagccg ttgcggcaac ataccgcggt tggcctacaa ccgcccggtg acgaccaaga
      121 ccaatagcac gatcagaacc aaaccgagtc cgccgctcgg gtagtagccc caaccgccgc
      181 tgtacggcca tgcgggcagt gcacctacca atagcagcag caggacaatc agtagaatag
      241 tgcctaagga catgttggac tcccttgatt cgcgaggttg agggaattta atcgcgaatc
      301 atcgaaatcg cagtcgggac gtcgcgccac ccactgtagg gtccgtccta ccgggccggc
      361 gctcccttgg gggcgagtat tgcctgccag ttgaggatga cattccacaa catgcgctga
      421 tcgccgcgat tcgagtcgcg gtacagcgga ttgaaattga acgcaacgac gtgcccctgc
      481 cccaccggca ggtcaaaaat cgccggacgg ccgagcaaat tgtcctcgcc ccagacttga
      541 ccgctgacca ggaacggcgc cggattctgc gggtcgcccc actgcagcac cacgccgcgt
      601 ggatcgaacg gcccgtcaag acaggtctgg caataggcca tccgcaacca acgccgcggc
      661 acggaataca gtgcgaagtt actccggaag acgtaggtgg agggcgtgta gccataggcg
      721 atggggtgat cggggcgcac gaagctcacg cgcacgtgcg cgccgggcgt gcgcgtgacg
      781 gactcgcgcg aagcctcgga cgaggacgcg ccaccgcccg ccgtgctgcg cggtacgccg
      841 cctgaatcgc ggcgcacacc gcgaacgatt ccgccttcga tgggcagcat cgtgccgttg
      901 ccgagtgtca cgagcaagcc gccgtgttcg atgtattgtt gaatttgcgc aagcccctgc
      961 caaccgatgc ccccggtaat gtcatcggag gccgccggcg tgccatggct cggcgtttgc
     1021 gcggtttttt tgaagggcat cggaccccat gccttgggaa ttccttggat ttgttccgcg
     1081 agttccaaat cgacgtggcc gtacaggagc acgtcgattt tgccctgcaa atgacctgct
     1141 cgaatgtctt cgtcacgtat atacacgtag ggaatgtgcc gctgatccaa ggaatagcgc
     1201 acccaaccga tcgaatccgt gtcggcccac ggtacccaaa cacccagccg cggcacgctg
     1261 gccgcgtgac gcgcgacctg cggcgccgcg gccgcgctca cgaaatccac gccttactgg
     1321 tcggcggtat cgcgcagcgc cgcggcgacc ccattttgag cggcaacaat ccaggagccc
     1381 gccgggtagc tcacgttgtc gacgacaaag gcgtgttccg cgatctcgac atgaaagcgc
     1441 gccagccggt agcgcgcctc gagtaatccc tcctgtcccg agtccttcag caagtacacc
     1501 ggacctgcgc ccagcaatcg tccgtgcggc tgcggcggca ccgtcaacag cgccaaaggc
     1561 atgtcgcgga tcgctcgatc ggcagtcgca atgacctcga gatggaaata cgccggcagt
     1621 tcccacgaca catcgtcgta cggctctccg gcatctttcg ggtaatgctg cggcgtcagc
     1681 agatccaccg cgtaattgcg gtagggttgg cccaatttga ccacataagt gccggccgga
     1741 aacgtgccct cggtgagcat caacggcaca tcggtccggc tgacctcgat gtgttgcgcc
     1801 tgcagccgcg cgaccagctg cgcgacgcgc gcagggtcgc cttgctcggc gggaatgatg
     1861 aacgcatacg gcggcgcgtc gagtccgcgc cgcaggctgt gcaggctctt ggtatagaaa
     1921 tttcgcagca gcatgcgcga gttggcggcg gcgtaatcga gcgccgccaa cgcggcggtt
     1981 tcgttgtagt tcacattgtc gcgcgcggac caggaaaaac tgccactcgg cggcggcact
     2041 acgcggtacc actggggcga ggtctcttcg gggctgagcg actgcgtcag cgtctccgcc
     2101 gtaccgttgc cgaaggtctc gtagccgcgc ccgatggcgt tgtggttcat ggccaccgaa
     2161 tcggtgaaca tatgcccgaa tccttcgccg aaattccagg tcgagacacc gggcattccc
     2221 aagccggtca gcgtctccac ctcgtgaaag ctcaacgcca gaaattctgt aaaggtgatc
     2281 ggatcgatgt tggggttgta cgggccggtg ccgttccaac tcatcaacag cggcacgctt
     2341 tcgtgcaagt cgtggacgat gacggggtga taatcgaaga acatgcggct cacggccttc
     2401 gaagtttgat gtgccagctg atgcgcatcg cgattgatgt cgacgaaggc gtacttggac
     2461 caatacggcg gcgattgccg cggcaacgaa tcacgatcgg tcttgccctt caagaagcga
     2521 taaaaccact ccacttgctt gtcgcggcca tcggggttgc acaccggatt gatgagcacc
     2581 accatggtct cgcgaattcg ccggatcatg ggctgttccg agaccgcaag ccggtagccg
     2641 agctcgagca ccgattcggt ggagccggtt tcgtccgaat gcagggccgc gttgaaataa
     2701 tagaaggggc gcgacccggc gatcaagcgt tcggcgtccg cgccgtccgt ggtgcgcgga
     2761 tcggcgagcg acgcattggc ggacttcatg gcatccaatt gttggatgcc tttctcatcc
     2821 gcgatggcca aaagaataat gtcgcggcct tcctccgatc gccctatggt gaacacccgc
     2881 acgcgcggcg acgcagccgc caatgcgcgg ctataggcat acgcctgggc cgagtcgacc
     2941 aattcgccgg gcgcgccgga aatgcgcccg aagaaggcgc gcggcgacgg aacagcggcg
     3001 gaggccggca gatacgcgac ccacggcgat agaaatcgtg tctcggtcgt ggccttcgcg
     3061 atctcctcga ccgaccccgg ttcggcgctg tcaggcgcga tcaagccctg tgccgagaca
     3121 tcgacgctcg cggcccacgc cgcgcacagg atcgcgatca accattttcg catgacacct
     3181 cacttgatgg gcggcgcgac caataccaat aaaacggcac gcctgccgcg agcagcatta
     3241 cgccgcgaaa cgcctcgtgc ggtgccgcga caaaagtatt gatcagcaac gcagccgtcg
     3301 ccaacagaaa aatcatcggt accacaggat agccgaacgc gcggtagggc cgcgccgcgt
     3361 cggggagttt gcgccgaaaa atgaatagcg acgcggtcac caatccatag aacagccacg
     3421 atgagaagat caccgaatcg gtcaacgcat cgtaggagcc ggaaagcgcc aacaggctgg
     3481 cccaagcggc ttgcgcgagg atggctcgaa cggggacgtt gctgcgcggc gaaagccgcg
     3541 tgaggctgcg aaagaacaga ccgtcgcgcg ccatcgcgaa cggaatgcgt gagttggcga
     3601 gtacgctggc atgcagcgcg ccgaacgaag agaccatcaa cgcgacggcg gtcatcgaaa
     3661 ccgccagcgg acccaaaaat ttcttcaaca cctcggtggc caccgacgag ttcaccgcca
     3721 cgctggcgat tgccacgggt gtcagcgcat agtagtaagc gatattgacg aacaaataca
     3781 aggcggcgac gatcagcgtg ccgccgacaa aggcgcgcgg caggttgcgc tgcggattgc
     3841 tgatctcacc cgcgagcggc gcgacattct gccagccgtc gtaggcccat aaggcgccga
     3901 gcatggccgc cccgaaaccg gcgatgccgc cgcgcgcgct ctgcgccacg cctgcacatg
     3961 tgccggatat cccggactgc gtcaaatggc cccagtcacc ggccgcgaag acaaaggcgc
     4021 cgacgcccac gccgagcacc aacccaacct tggcaatggt gagcgcaagg gccatgcggc
     4081 caccggcggc cacggaggcg caattgatga gcgccacgat ccagatggcg gcgagcgcca
     4141 ccatggtcaa gccgttcaag tgcgggccga gggtgcccgc ggcgtgccat cggtccaagg
     4201 cgccgcccaa cgccacattc atgaagatcg ccagacccac tgcgagtgcc gcctgcgatc
     4261 cggcgcgagc gacggtaaaa caaatccatc catagagaaa gccgaccagc cgcccatacg
     4321 cgcggcgcag gtatacgtag tcgccgcccg cctccggcat catcgcggcg atctccgagt
     4381 acgagaaagt acctgccagc gacaacaatc cggccgccag ccaaaccgcg agcacgagtt
     4441 tcgcgctgcc gacattgcag gtcatgacgc gagtcttgag gaagacgccg gtgccgatca
     4501 tgttggcgac gttgactgcg gtcgccgccc aaggaccaac gccgcgggtg agttcgccgc
     4561 gtacggcact tgctgtggat gcagcgctca tgtctgctag tatcgaacaa atgcaaccgg
     4621 acttttcgca attcggcccg ctgctattgg ccgccttggt ggtcttcgct gtgtaccgcc
     4681 ggtttcggcg cagcttcgga cggcagctgc tcagtccgaa gcgcatgact tttcgcatcg
     4741 tgctgcttgc ggtgatcggc tgcgcactgt tgccgctggc gctgcgctcc acgcagtact
     4801 tgggcgcgga gctgctcggg gcggcgcttg gcgcggcgtt gggatggtgg ggtgccgagc
     4861 gaacgcggtt tgtgcgcgat gcgggtcgcc tgttctacgt gccgcatacc tatacgggaa
     4921 tcgcggtgtc gctgttgttc ttggggcgac tggtgtttcg gctgctgcag gcttacggcg
     4981 cccacgcaca ggcctcgcag gcgttgtcga cgcccgcgat ggtgcgaagc ccactcacgg
     5041 tgggaatctt cttcgtgctg ctgggttact acctgtattt ctacagctgg gtgctgtgga
     5101 aatctaatcg tctcgatgtc tcagacatcg aggccgcctc ccccgccgcg gcgcagtaaa
     5161 tccgggctca cgcgcgcgac gcgtggccct cgctcgttaa ttgagcaatg cgaaaaacag
     5221 cacgcctgtc atcagcgcta agccggctaa cagtgtgtta tggccccagc gggctatcgt
     5281 tccaatcatt tccgtctcct aatttacact catcgacaac cgccacaggt ccgtggcggc
     5341 caacgagagt tatgggggcg gaactgtaca tttccagtag cggaaa
//
LOCUS       DASWZT010000201        11390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_93475, whole genome shotgun sequence.
ACCESSION   DASWZT010000201 DASWZT010000000
VERSION     DASWZT010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11390
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93475"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /gene="gfa"
                     /locus_tag="VGE92_05405"
     CDS             complement(<1..493)
                     /gene="gfa"
                     /locus_tag="VGE92_05405"
                     /EC_number="4.4.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF003829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051907 - S-(hydroxymethyl)glutathione
                     synthase activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione synthase"
                     /protein_id="HEY1313290.1"
                     /translation="MAVSIHPSVDNGVKQGSKDFAGGTLSCKCTDRPVKVSIGAQTAH
                     NHACGCTKCWKPKGAKFSVIAVVSKDKVNVTQNGDKLKVVDPSATIQRHACKVCGVHM
                     FGRIENAKHAFYGLDFVHTELSPESGWSAPGFAAFVSSIIEAGEDPANMGAVRARLKE
                     LGLE"
     gene            complement(532..1641)
                     /locus_tag="VGE92_05410"
     CDS             complement(532..1641)
                     /locus_tag="VGE92_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEY1313291.1"
                     /translation="MKTRAAVAFEADNPLSIEEVDLAAPKTGEVLVRIVATGVCHTDA
                     FTLSGADPEGLFPVILGHEGGGIVEEVGSGVTSVKAGDHVIPLYTPECGTCEFCLSGK
                     TNLCQRIRVTQGKGVMPDGTSRFSYKGKPILHYMGTSTFSEYTVLPEISLAKINPKAP
                     LDKVCLLGCGITTGIGAVLNTAKVTPGSTVAVFGLGGIGLSVVQGAVMAKASRIIAVD
                     LNPTKWTMAKSLGATDYVNPKDYDKPVQQVIVDMTNGGVDYSFECIGNVHVMRSALEC
                     CHKGWGESVIIGVAGAGQEISTRPFQLVTGRVWRGSAFGGVKGRSQLPGYVDRYLAGE
                     IKIDEMISEVLPLEQINEAFELMHAGKVIRSVIKY"
     gene            2259..3515
                     /locus_tag="VGE92_05415"
     CDS             2259..3515
                     /locus_tag="VGE92_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313292.1"
                     /translation="MNTRIKLAIAAGAAMWVVPMTQAVAGIDVQAGDWKIDFSGNVNG
                     FYTSASCDTPSATTTVVGGFACAGDNSSSIRNGLLPAALVFSATTRQDNFDIDVTIGF
                     YPGINSSAAGQVNLGGGSPTALATPGIDARQAFFTFGDASWGTVKIGRDIGLFGKDAI
                     LDDMTLLGMGTASGNAAPSNTSLGRIGLGYIYTDWEPQITYTSASFSGFSFTAGIFQP
                     LDEFNQDNGLDYSRHTAPQIQAGLGYTFGDPKSDGVNGKVWLDVVSQRYQLPSGTVIP
                     GASSANTGTAVDGGVKVDVAGFEAVLYGYSGKGVGTTGLYLDAVSPGGDTRKSDGGYV
                     QATYKFDKLKVGLSYGISDLKLADDEVSTGNPLLKRNASGVFGLYYSLTKSVTLVGEY
                     INTKSTAQGGNEAKENDFALGGILFF"
     gene            3606..4391
                     /locus_tag="VGE92_05420"
     CDS             3606..4391
                     /locus_tag="VGE92_05420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HEY1313293.1"
                     /translation="MNRLARYAAPSLAATLFAAGPCHAIGEEQQPSGSAWELLRAQYY
                     GERPMGVVDETYMSLQVPANTPDPAATPLTLRFADDEHRRIKRVRVFIDNNPSPLVAT
                     FDFPGTPLTEIDMRVRIDRFTSVRAIAETADGALEMRSSWVKASGGCSAPPAASSAGL
                     LGEIRVRTSPDAKSLQVGIRHPNASGFQIDPRSGDPIPAHYITHIRVNAGGAPLLDAD
                     TGISLSENPSLRIASDKPLAAPISVDAVDSETQAHYTASNGSR"
     gene            4378..5304
                     /locus_tag="VGE92_05425"
     CDS             4378..5304
                     /locus_tag="VGE92_05425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein relay system zinc metallohydrolase
                     2"
                     /protein_id="HEY1313294.1"
                     /translation="MGAAKARIALCLACPLAFAADAPPAAFNLSEPAPGVFVHLGRQL
                     ALDVPGHDDIANIGFIVGAKCVAVIDSGGSVSVGRELRAAIVKRTPLPICYVINTHVH
                     VDHVLGNGAFGQDKPSFVGNSTLADAIARSRAFFVREYSADLDAPPSAAQVIGPDRLV
                     ERELSLDLGGRSLALRAWPTAHSDSDLTVYDPKTATLWTGDLLFRERLPALDGSLAGW
                     LALLDQLTKIGARLAVPGHGSMTRDAPAAIREERRYLQALADGVRVELKRNESIEHAI
                     AHVAASEQAHWLLWDKVHPHNVVRASQEAEWQ"
     gene            5304..6566
                     /locus_tag="VGE92_05430"
     CDS             5304..6566
                     /locus_tag="VGE92_05430"
                     /inference="COORDINATES: protein motif:HMM:NF044548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1313295.1"
                     /translation="MRRRSFLQLSAGCAGATLARAVRARTATARAGAKVVIVGGGFAG
                     ASCALTLRQLDPGLHVTLVDPDERYVTCPMSNSALVGLRDLKSLYVSRRGRADGGVRY
                     VQSQVTAIDTERRRAQLAGGGVLGFDRLVLAPGIRFLWNKPQGYDAAASRSMPHAWQA
                     GAQTEILAAQLSAMRDGGVVAISVPTGPMRCPPGPFERAGLIAGYLKQNKPRSKVLIF
                     DGNNAFPKQDAFNDAWHALYPGMIEWIPVVADGAVVRVDPAAKILFTANAQHRVDVAN
                     IIPPQAPGLLAQQTGLASDHGWCPVKAESFESSLIAGVHVIGDACIADPMPKAASAAN
                     AQARQCARAIVAALDGREAPAAEYSSVCYSMLARGRALSIHGEYRVADGVIRQMSAAQ
                     DAPPVTAEQEARNAEEWYRRIVRDSFGA"
     gene            complement(6589..6924)
                     /locus_tag="VGE92_05435"
     CDS             complement(6589..6924)
                     /locus_tag="VGE92_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313296.1"
                     /translation="MKTICVMVVMASLAAASFAQTAPTPAAARTGGIWKAAVPPTPMR
                     GEFDSLDPLGVAAGARIQADCSLNWVSPDDGKLYCFSSGTSLEYFLDQPQATLERARA
                     NWQKMRERH"
     gene            7020..8369
                     /locus_tag="VGE92_05440"
     CDS             7020..8369
                     /locus_tag="VGE92_05440"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY1313297.1"
                     /translation="MWRYGACLVFIATFAGCVRYQAAPLEPKYSADEFAARRLDLGPA
                     REWDRAQLLAMALTQNPQLAVARAEVKAALAHEISAAQAPNPDLTLQSEYARHDAHPW
                     LYGVSLNWLLRSPERRRLDMAIAGFDTGNARLHLMDRAWSVRSALAAALSDWESARRR
                     SSLLERLATAQDRLVALERQRVDAGEDSPNELLTSEQARIAIDRERAELRETIDAAQA
                     ASAKALGVPPQALDGLAIAWPEWGEPPPLEEDARRASREQALLSRADLGAAIGEYAAA
                     EAKLQLSIARQYPQFTLSPGYYWDHGIAKFPFDVGFTLPFNRNQGEIAEARSDRELAG
                     QRMLALQAEIYGEIVAAERAEQTARSSAEAAERQLQTARRQQQAADLGLRLGAGDLQE
                     QLAAQIIAMRAELEVLQMRTQLQTSRNNLEDVLHTPLSGPELALAGSMSSLALGAGS"
     gene            8366..9358
                     /locus_tag="VGE92_05445"
     CDS             8366..9358
                     /locus_tag="VGE92_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313298.1"
                     /translation="MSACARGTCARGMLALACSAMLGMSLLAIADDDDKDKDHDAAQP
                     AAQPSLNDEQQRAVGIVVAHPIPAKAPDRIEAIGVVLDETTLISDMGDANAASAAERS
                     ASAELNRLHGLYNGGAGASLRMLEAAQTEQAKAQAQAEIAAAKFAQHWGPLNSLSPDA
                     RQKVIEALRHGHGLLLRADLPGRHTLGSNPAKALLDVDGIEVPGRVLGALRQTTELQS
                     VGLLIDVPNAPQGLGPGARVPVALLSDERGGLLLPRDALLYDENGAYVYKQLSKKSAA
                     EKTRYAPVKVTLLLAVGDGWLVKGVDDDDAIVVHGAGVLWSLQGVGAQPQDDDD"
     gene            9371..>11390
                     /locus_tag="VGE92_05450"
     CDS             9371..>11390
                     /locus_tag="VGE92_05450"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1313299.1"
                     /translation="MLAAIVRASLRNPRIVTALACLIAALGLAALLAARFDVFPDFAP
                     PHVLVQTEAPGLDATQVEALVTRPLEGLLAGTENVKSVRSTSSQGLSAIQVVFDRGGD
                     PYRQRQVVTERLAEFAGLLPPGVGAPLLSPLSSSMEYLVHFGYTSDRLSPVELRDVVQ
                     WIVKPQILAVPGVAQAQIFGGEVRERQIQIDPAKLAAANLSLDDVLAAAKRATELIGG
                     GYLETTTQRIVIQAQAPGATLEALAQAVVATHDGNAVRLGDVATLREGAEPRFGDALI
                     AGKPGILIETSTQFGANTLEVTRALEERLDALAPALAKQGVRYHPALLRPASFIESAI
                     EKLRNSIGVGAVLVVGLLLLTLHDWRGALVSFSAIPLALLATVWILQAWGLSLNTMTL
                     GGLVVALGVVVDDAVIDVENILRRRRIAADNVDIGELFIGASLEVRRPVFYATAAVAV
                     AFLPILMMSGLQGAFFRPLSIAFLLAVGLSLLVAMSATPALCALVMAKHVPKAEASAL
                     KYLKRGQLRVIGWLERRPRTLLAIVLASGLAGIVFLPLLGSRLLPDFRENYLIAHASL
                     RPGISLAETTRVGVQISKGLIAIPGVKSVAEQIGRAENGQDPDAPNKSEFEVQIDPLK
                     QHSASEIDAAIRDVFDDYPNQLVEIYSVLAERIGETLSGESAPFSISVI"
BASE COUNT         2052 a         3553 c         3699 g         2086 t
ORIGIN      
        1 gctcgagacc cagctctttc agccgggcgc gcaccgcacc catgttcgcc ggatcctccc
       61 ccgcctcgat gatcgaggag acaaaggcag cgaaaccggg cgccgaccag ccggactccg
      121 gcgacaattc ggtgtgcacg aaatcgaggc cgtagaacgc atgcttggca ttctcgatcc
      181 ggccgaacat gtgcactccg cacaccttgc aggcgtggcg ctgaatggtt gccgatggat
      241 cgactacttt gagcttgtcg ccgttctgcg tgacattgac cttgtccttc gacacgaccg
      301 caatcaccga gaacttcgcg cccttcggct tccagcactt ggtgcagcca caggcgtggt
      361 tgtgcgcggt ctgcgcaccg atcgacacct tgaccggccg gtcggtgcat ttgcaggaca
      421 aggttccccc tgcgaagtcc ttcgatccct gcttgacacc gttgtcgacc gacggatgaa
      481 ttgaaactgc catgattcct cctaactggt tttcttgact ctcgcgctcg ctcaatactt
      541 gatcaccgaa cgaataacct tacccgcatg catcaactcg aaggcctcgt tgatctgttc
      601 caacggcagc acttcgctga tcatctcgtc gatcttgatt tcgccggcga gataacgatc
      661 cacgtagccc ggcaattgcg agcggccctt gacgccgccg aaggccgagc cgcgccagac
      721 gcggccggtg accaactgga acggccgggt ggagatttcc tgacccgcgc cggcaacacc
      781 gatgataacc gattcgcccc accccttgtg acagcactcg agtgccgagc gcatcacatg
      841 cacgttgccg atgcactcga acgagtaatc gacaccgcca ttggtcatgt cgacgatcac
      901 ctgctgcacc ggcttgtcgt agtccttcgg attgacatag tcggtcgcac cgagcgactt
      961 cgccatggtc catttggtcg ggttaaggtc gaccgcgatg atgcgcgacg ccttcgccat
     1021 cacggcgccc tggaccacgg acaggccgat accgcccaaa ccgaaaacgg caacggtcga
     1081 tcccggcgtg actttcgcgg tgttgagcac cgcgccgatt ccggtggtga tgccgcaacc
     1141 gagcagacac accttgtcga gcggcgcctt cggattgatt ttcgccagcg aaatttccgg
     1201 cagcaccgtg tattcggaga acgtgctggt acccatgtaa tggagaatgg gcttgccctt
     1261 gtaagaaaag cgcgacgtgc catccggcat cacgccttta ccctgggtaa cgcgtatgcg
     1321 ttggcacaag ttcgtcttgc ccgacagaca gaactcgcac gtcccgcatt ccggcgtata
     1381 caaaggaatg acatggtcgc cggctttgac gctggtgacg ccggatccga cttcctcgac
     1441 gatgccgccg ccctcatggc ctagaatcac ggggaacagg ccttcggggt cggcgccgga
     1501 cagcgtgaag gcatcggtat gacacacgcc ggtcgcgacg atgcgcacca gcacctcgcc
     1561 ggtcttgggc gcagccagat cgacttcctc gatgctcagc gggttatcgg cttcgaacgc
     1621 aacggccgct cgcgtcttca tggattacag ctcctcggta acaactcggg tggcaactca
     1681 tggccaactc tagcgacaag cagcaagcgt gccagccgca catcgcgcgc agatagcgct
     1741 gattgatcag caacttgcga ggtgagacat aacacggcgc cggcgcaacg ctgcccggtg
     1801 tttctgaacc gtcacagttt gtgcctcaaa aatgaggcag cgcggagctt acgatcttgg
     1861 catcgcatgg acgcgcttga ttcgctcaat cgggtgcctg tgtctaatcc aacacagaat
     1921 gcgacaaatc tgatgctccg ggtgacactg atgcgtcaaa gcaacaggtg aagatgcgat
     1981 ggtgcggggt gggtgtgggc tgccgccgaa gtcgttgaat tggcactttg ctcggtaatt
     2041 accttgtcgg agagcgggct tgtggcaaga actccgcggg tcctaaggcc tccgctgcga
     2101 ccctggttgc agcattggta cgcctcttgc agctaccaaa gcagggcttc acccgacgtc
     2161 aggtggcgtt tggcgagtgc gggaaaagct gcggtcggct ttaggtacta aaccgtaagc
     2221 ctgcgatgaa ctaaagaact cgaacggagg gaggtttcat gaatacgcgt atcaagctag
     2281 cgatcgcagc tggcgccgcg atgtgggtcg tgccaatgac tcaggcggtt gccggaatcg
     2341 atgtccaagc gggcgattgg aagatagatt tttccggcaa tgtgaatggt ttctacactt
     2401 cggcgagttg cgacacgccg agcgcgacca cgaccgtggt cggcggcttc gcttgcgccg
     2461 gcgataactc ctcgtcgatt cgcaacggtt tgctaccggc agcgctggtg ttcagcgcca
     2521 cgacccgcca ggataacttc gatatcgatg tgaccatcgg gttttatccc ggcatcaaca
     2581 gttcggcggc cggccaagtc aacttgggcg gcggcagtcc gaccgctttg gcgacacccg
     2641 gcatcgatgc acgccaggcc ttcttcacct tcggcgatgc cagctgggga accgtgaaaa
     2701 tcggccgcga catcggtctg ttcggcaagg atgcgattct cgacgacatg accttgttgg
     2761 gcatgggcac ggcatccggc aatgcagcgc cgagcaatac cagcctcggc cggatcggct
     2821 tgggctacat ctatacggat tgggaaccgc agatcacgta tacctcggcg agtttcagcg
     2881 gcttctcgtt tacggccggt atcttccagc ccttggacga attcaatcag gacaacggcc
     2941 tggattattc gaggcacaca gctccgcaga tccaagcagg gttgggctac acgtttggtg
     3001 atccgaagag cgacggcgta aacggcaagg tctggttgga cgtggtcagt cagcggtatc
     3061 aactgccctc agggacagta attcccggag cttcttcggc aaacaccggc actgcggtcg
     3121 atggcggggt gaaggtggat gtggcggggt tcgaggcggt gctgtacgga tacagcggca
     3181 agggcgtcgg gacgaccggc ctgtatctgg atgcggtctc tccgggcggc gacacgcgca
     3241 agtccgacgg tggctacgtg caggcgacgt ataaattcga caagctgaaa gtcggcttga
     3301 gctacggcat atcggacctc aagctcgcgg acgatgaagt ctcgaccggc aatcccttgc
     3361 tcaagcgcaa tgcgtcgggc gtgtttggtc tgtactacag cctgacgaag agcgtgaccc
     3421 tggtcggcga gtacatcaat accaagtcga ccgcccaggg cggcaacgag gccaaggaga
     3481 acgatttcgc gctcggaggc attctgttct tttgatttag gtgggcgatg tagtcacggt
     3541 ggttcgagtc ggcgacgcag cgaatgcgtc gccgattttc gagcgggttg tgtcacacta
     3601 agggcatgaa ccgcctagcc cgctacgccg cgcccagcct ggccgcgacg ttattcgccg
     3661 caggaccctg ccatgcgatc ggcgaagagc agcaaccctc cggcagcgcc tgggaattat
     3721 tgcgcgcgca atattacggc gaacgcccga tgggcgtggt cgatgagacc tacatgagtt
     3781 tgcaggtgcc cgccaacacg ccggatccgg ccgcgacgcc gctcacgctg cgctttgccg
     3841 atgacgagca tcgacgcatc aagcgggtgc gcgtgttcat cgacaacaat ccctcgccgc
     3901 tggtggcgac cttcgatttt ccgggcacgc cgctgaccga aatcgacatg cgcgtgcgca
     3961 tcgatcgatt cacctcggtg cgcgcgatcg ccgagacggc cgacggcgct ttggaaatgc
     4021 gctcaagctg ggtgaaggcg agcggcggct gctccgcccc gccggccgcg tcaagcgcgg
     4081 gtctgctcgg cgagattcgc gtgcgaacct cgcccgacgc caagtcgctg caagtgggaa
     4141 ttcgccaccc gaacgcgtca ggcttccaaa tcgatccgcg cagcggcgac ccgattccgg
     4201 cgcactacat cacgcacatc cgggtcaatg ccgggggtgc gcccctgctc gacgccgata
     4261 cgggtatttc cctgagcgag aatccctcgc tgcgcatcgc ctccgacaag cccctggctg
     4321 cgccaatcag cgtcgatgcc gtggattcgg aaacccaggc ccattacacc gcgtccaatg
     4381 ggagccgcta aggctcgcat cgctctttgt ctcgcctgcc ccctggcgtt cgccgcggac
     4441 gcaccgccgg ccgctttcaa cttgagcgag cccgcgcccg gtgtgttcgt gcatctcgga
     4501 cgtcagttgg cgctcgatgt gccggggcac gatgacatcg cgaatatcgg tttcatcgtc
     4561 ggtgcgaagt gcgtcgccgt catcgacagc ggcggatcgg tcagcgtcgg gcgggagctg
     4621 cgcgccgcga tcgtcaaacg cacgccgttg ccgatttgct atgtgatcaa tactcacgtg
     4681 catgtcgatc acgtgctggg caatggcgca ttcggccaag acaagccgag cttcgtgggc
     4741 aattcgacgc ttgcggatgc catcgcccgc agccgcgcgt tcttcgtccg agaatattca
     4801 gcggacctcg atgcaccgcc gagcgcggca caggtgatag gtcccgaccg actggtggag
     4861 cgggagctca gcctcgatct cggaggccgt tctttggcgt tgcgtgcgtg gccgacggcg
     4921 catagcgaca gcgaccttac ggtctatgac ccgaaaaccg ctacgctgtg gaccggagat
     4981 ttattgtttc gcgagcgcct gccggccttg gacgggagcc tggcgggctg gcttgcgcta
     5041 ctcgatcagc tgacgaagat cggggcccgt ctcgccgtgc ccggtcacgg atcgatgacg
     5101 cgcgatgcac cggcggcgat ccgcgaggag cgccgctacc tgcaagcgct ggccgacggc
     5161 gtgcgcgtgg aactcaagcg caacgaatcg atcgagcacg ccattgcgca cgtcgccgcc
     5221 tccgagcaag ctcattggct gttgtgggac aaagtccatc cgcataacgt agtgcgtgcc
     5281 tcgcaagaag ccgaatggca gtgatgcggc gacgctcatt tctgcagcta tcggccggct
     5341 gcgcgggcgc gacgctcgct cgagccgtgc gggcgcggac ggcgactgcg cgcgccgggg
     5401 ccaaggttgt gattgtcggc ggcggattcg ccggcgcaag ctgcgcactc accctgcgcc
     5461 aactcgaccc gggcctgcat gtcacgctcg tcgatccgga tgagcgctac gtcacctgtc
     5521 ccatgagcaa cagcgcactg gttggattgc gcgacctgaa gtcgctgtat gtgtcgcgcc
     5581 gcggccgcgc ggacggcggc gtccgctacg tgcaaagcca agtgacggcc atcgacacag
     5641 aaaggcgccg ggcgcagctt gcgggcggcg gcgtgctcgg gttcgaccgc ttggttttgg
     5701 cgcccggaat ccgcttcctg tggaacaagc cgcagggcta cgatgcagcc gcgtcgcgct
     5761 cgatgccgca cgcctggcag gccggtgcac aaaccgaaat cttggcggcg cagctctccg
     5821 ccatgcgcga tggcggcgtg gttgcgatca gcgtgcccac cggacccatg cgatgtcctc
     5881 cgggaccgtt cgagcgtgcc ggcctcattg ccggttactt gaagcaaaac aagccgcgct
     5941 ccaaagtgct gatcttcgat ggcaacaatg cctttccgaa acaggacgcg ttcaacgacg
     6001 cctggcatgc gctctacccg ggaatgatcg agtggatccc ggtcgtcgcg gacggcgcgg
     6061 tcgtgcgcgt ggatcccgcg gccaaaattc tatttacggc gaatgcgcag catcgcgtcg
     6121 atgtggccaa catcattccg ccgcaggcgc cggggttgct ggcgcaacag accggactcg
     6181 cgtccgatca tggatggtgt cccgtcaagg ccgagagctt cgagtcctcg ttgatcgcgg
     6241 gcgtgcacgt catcggcgac gcgtgcatcg cggatccgat gcccaaagcc gcgtccgcgg
     6301 cgaacgccca agcccggcag tgcgcccgcg cgatcgtcgc ggccttggac ggacgcgagg
     6361 cgcctgcggc cgagtattcg agcgtttgct acagcatgct ggcgcgtggc cgcgcgctgt
     6421 cgatccacgg tgagtatcgc gtcgcggacg gcgtgatccg gcagatgagc gctgcccaag
     6481 atgcgccgcc ggtgacagcg gaacaagagg cccgcaacgc cgaggaatgg tatcggcgca
     6541 tcgtgcgcga ctcgttcggc gcataggcgg cggcgcacga cttgtgattc agtgccgctc
     6601 gcgcattttt tgccaattgg cgcgagcgcg ctcgagcgtc gcctgcggct ggtcgagaaa
     6661 gtactcgagc gaggtgccgc tcgagaagca atacagcttg ccatcgtccg gactcaccca
     6721 gttgagcgaa cagtccgcct ggatcctcgc tcccgcggca acaccgagcg gatcgagact
     6781 gtcgaattcg ccgcgcatcg gtgtcggcgg gaccgcggcc ttccagatcc cgccagtgcg
     6841 cgccgcggcc ggcgttggcg ccgtttgtgc gaagctcgcg gcggccagcg aggccatgac
     6901 aaccatgacg caaatcgttt tcaagtgttc catttccgtg acacgggttt tatctgcctg
     6961 tccgtacgag tattattcca aaaaaaggga aacactcccg ggcgtcatag gaaaaacaca
     7021 tgtggcgcta cggcgcttgc ctcgtcttca ttgcaacatt cgccggctgc gtccgctatc
     7081 aagcggcgcc gctcgagccg aagtatagcg ccgatgaatt cgcggcacga cgccttgatc
     7141 tagggccggc acgcgaatgg gatcgcgccc aattgctggc catggcgctc acgcaaaacc
     7201 cgcaactcgc ggtggcccgg gccgaggtca aggcggcctt ggcgcatgaa atcagcgccg
     7261 cgcaagcgcc aaatcccgat cttaccttgc aatccgagta cgcgcggcac gatgcgcatc
     7321 cgtggctcta cggggtgagc ttgaattggt tgctgcgcag ccccgaacgg cggcggctgg
     7381 atatggcaat cgccggattc gataccggca acgcgcgctt gcatctgatg gatcgggcat
     7441 ggtcggtgcg cagcgccttg gccgcagcat tgtcggactg ggaatccgcg cggcggcgct
     7501 cgagcttgct cgagcgtctg gcgacagcgc aagaccggct cgtcgcactt gaacggcaac
     7561 gcgtcgacgc cggcgaggat tcacccaatg aactcctgac gagtgagcag gcgcgaatcg
     7621 cgatcgatcg agaacgggcc gagctgcgag aaacgatcga tgcggcccag gcggccagcg
     7681 ccaaagcact gggtgtgccg ccgcaggcgc tcgatggcct ggcgattgcg tggccggagt
     7741 ggggcgagcc gccgccgctc gaggaggacg cgcggcgtgc gagccgcgag caggcactct
     7801 tgtcgcgcgc cgatctgggc gcagcgatcg gcgaatatgc agcggccgaa gccaagttgc
     7861 agctgtcgat cgcgcgccaa tatccgcagt tcacgctctc gccgggctat tactgggatc
     7921 acggcatcgc aaaatttccg ttcgacgtcg gcttcacttt gccgttcaat cgcaaccagg
     7981 gcgaaatcgc cgaggcgcgc tccgatcggg agctcgcggg gcagcgcatg ctggcgttgc
     8041 aagccgaaat ctacggcgag atcgtggccg ccgagcgcgc agagcagact gcgcgctcga
     8101 gcgccgaggc ggcagaacgg cagctgcaga cggcgcgccg tcaacaacag gcagccgatc
     8161 tcgggctgcg tttgggcgcg ggcgaccttc aggagcagct cgccgcacag atcatcgcga
     8221 tgcgcgccga gctcgaagtg ctgcaaatgc gcacgcaact acaaacttcg cgcaacaatc
     8281 tcgaggatgt cttgcacacg ccgctgtcgg gtccggaact tgcgctggca gggtccatgt
     8341 cgtcgctggc cttgggagcc ggctcgtgag cgcgtgcgcc cggggcacgt gcgcccgggg
     8401 catgttggcg ctcgcttgca gcgcaatgct gggcatgtcg ctgctcgcga ttgccgacga
     8461 cgacgataaa gacaaagacc atgatgccgc tcaaccagcg gcgcagccga gcttgaatga
     8521 cgaacagcag cgtgcggtcg gtattgtggt ggcgcatccg atacccgcca aggcgccgga
     8581 tcgtatcgag gcgatcggcg tcgtgctgga tgagacaacc ttgatatccg atatgggcga
     8641 tgccaacgcg gcgtccgcgg ctgagcgctc ggcgtcggca gaacttaacc gcttgcatgg
     8701 cctgtacaat gggggtgccg gcgcatcgct gcgcatgctg gaagcggcgc aaaccgagca
     8761 agcgaaggca caagcgcagg ccgagattgc ggcggcgaaa tttgcgcagc attgggggcc
     8821 gctcaactcc ctgtcgccgg atgcccgcca gaaggtgatc gaggcgctgc ggcacgggca
     8881 cggattgttg ctgcgcgcgg acctgccggg gcggcacacg ctcggcagca atccggccaa
     8941 agccctattg gacgtggacg gcatcgaggt tcccggccgc gttctcggcg cgctgcgcca
     9001 aacgaccgag ttgcagagcg tgggattgtt gatcgacgtg ccgaacgcgc cgcagggtct
     9061 aggccccggt gcgcgcgttc cagtggcgct gttgagcgat gagcgcggcg gattgttact
     9121 gccgcgcgac gcattgctgt acgacgagaa cggcgcttac gtctacaagc aattgagcaa
     9181 gaaaagcgcg gccgagaaga cgcgctatgc gccggtgaaa gtgacgttgc tgcttgccgt
     9241 cggcgatggc tggctggtca agggtgtcga tgatgacgac gccatcgtcg tgcacggcgc
     9301 gggcgtgttg tggtcgctgc agggagtagg tgcccagccg caggatgatg acgactaggg
     9361 acgcaacgcc atgctcgccg ccatcgtccg cgcctccttg cgcaacccgc gcattgtcac
     9421 cgcgctcgcc tgcctgatcg ccgcgctcgg gctcgccgcg ttgctggcgg cacgtttcga
     9481 tgtgtttccg gatttcgcgc cgccgcatgt gttggtgcag accgaggcgc cggggctcga
     9541 cgcgacgcag gtcgaggcgc tggtcacgcg gccgctcgag gggctcctcg ccggcaccga
     9601 gaatgtgaag tcggtgcgtt cgacctcgag ccagggtctc tccgcgatcc aagtcgtgtt
     9661 cgaccgcggc ggcgatccgt atcgccaacg tcaggttgtg accgagcgcc tggcggagtt
     9721 cgccgggttg ctgccgccgg gcgtcggagc gccgctgttg tcgccgctca gttcctcgat
     9781 ggaatacctc gtgcacttcg gctacaccag cgaccgtctc tcgccggtgg agctgcgcga
     9841 tgtggtgcaa tggatcgtca agccgcagat tctcgcggtc ccgggcgtcg cgcaggcaca
     9901 gattttcggc ggcgaggtgc gcgaacggca gattcaaatc gatccggcga aacttgccgc
     9961 cgctaatttg agcctcgacg atgtgctggc cgccgccaag cgcgccaccg agttgatcgg
    10021 cggcggctat ctcgaaacca ccacgcaacg catcgtgatc caggcgcaag ccccgggggc
    10081 gacgctcgag gcgctggcgc aggcggtggt tgcgacgcac gatggcaacg ccgtgcgttt
    10141 gggcgacgtc gccaccttgc gcgaaggcgc ggagccgcga ttcggcgacg cattgatcgc
    10201 cggcaagccg ggcatactga tcgaaacctc gacgcagttc ggcgccaaca ccttggaggt
    10261 gacgcgcgcg ctcgaagagc gtctcgatgc gctggcgccg gccctcgcca agcagggcgt
    10321 gcggtatcat ccggcgctgc tcaggccggc gagcttcatc gagagcgcga tcgaaaagct
    10381 gcgcaattcc ataggcgtag gcgcggtgct ggtggtcggg ctgttgctgc tcaccttgca
    10441 cgattggcgc ggcgcgctgg tctctttcag cgcgattccg ctcgcgctgc tcgccacggt
    10501 gtggattctg caagcctggg gattgagtct caacaccatg acgctcggcg gcctcgtggt
    10561 cgcgctcggc gtggtggtcg acgatgccgt catcgatgtg gagaacattc tcaggcgccg
    10621 ccgcattgcg gccgacaatg tcgacatcgg cgaattgttc atcggcgcct cgctcgaggt
    10681 gcgccgcccg gtgttctacg caacggcggc cgtagcggtg gcctttcttc ccatcttgat
    10741 gatgtccgga ttacagggcg cgttctttcg ccccctgtcg atcgccttcc tgctcgccgt
    10801 cggcttgtcg ttgctggtcg ccatgagcgc aacgccggcg ctgtgcgcgc tggtcatggc
    10861 caagcatgtg ccgaaggccg aagcctcggc gctcaagtac ttgaaacgcg ggcagctgcg
    10921 cgtgatcgga tggctcgagc ggcggccgcg gacgctgctc gccatcgtgc tggcaagcgg
    10981 gctcgccggg atcgtattcc tgccgctgct cgggtcacgc ttgctgcccg attttcggga
    11041 gaattacctg atagcacatg cctcgttgcg ccccgggatt tcgctcgccg aaaccacccg
    11101 cgtcggcgtg caaatctcca aggggctcat cgccataccc ggggtcaaga gcgtcgccga
    11161 acagatcggc cgcgcggaga acggtcagga cccggatgcg cccaacaaga gcgagttcga
    11221 agtgcagatc gatcccttga agcagcacag tgcgagcgag atcgatgcgg cgattcgcga
    11281 tgtgttcgac gactacccca atcaattggt ggagatttac tcggtattgg ccgagcgcat
    11341 cggtgaaacg ctgtccggcg agtcggcgcc gttttcgatc agcgtaatag
//
LOCUS       DASWZT010000202         6554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_78889, whole genome shotgun sequence.
ACCESSION   DASWZT010000202 DASWZT010000000
VERSION     DASWZT010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6554
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78889"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VGE92_05455"
     CDS             <1..419
                     /locus_tag="VGE92_05455"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY1313300.1"
                     /translation="ADKGPLAQKLGAQIYIDSTAQNAAEELTKLGGAKVILATVTNAK
                     SMSAVVPGLGIDGKLLVVGASGEPIEVPPFLLIGARRSIAGWPSGTAPDSEDTLKFSV
                     KSNVRPMIETFPLAKAADAYQHMMSGKARFRVVLIP"
     gene            complement(630..705)
                     /locus_tag="VGE92_05460"
     tRNA            complement(630..705)
                     /locus_tag="VGE92_05460"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(670..672),aa:Lys,seq:ctt)
     gene            complement(756..831)
                     /locus_tag="VGE92_05465"
     tRNA            complement(756..831)
                     /locus_tag="VGE92_05465"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(796..798),aa:His,seq:gtg)
     gene            complement(863..939)
                     /locus_tag="VGE92_05470"
     tRNA            complement(863..939)
                     /locus_tag="VGE92_05470"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(903..905),aa:Arg,seq:tct)
     gene            complement(1079..1429)
                     /locus_tag="VGE92_05475"
     CDS             complement(1079..1429)
                     /locus_tag="VGE92_05475"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY1313301.1"
                     /translation="MSPRRKVTSRNMSLRDSAPIFAALGDEMRLRLMTTLCVGGVMSI
                     AQLTAGTQITRQAITKHLDVLAAAGLVRDVKVGRERLWEFEPAQLDEARRWLEGIAQQ
                     WDHALDKLKRAVES"
     gene            complement(1413..1871)
                     /locus_tag="VGE92_05480"
     CDS             complement(1413..1871)
                     /locus_tag="VGE92_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY1313302.1"
                     /translation="MGSTDRIERKILLRATRARVWRALSDAKEFGDWFGVDFKGKSFE
                     AGKSVKGQITYPGYEHIVMEVMIDRVIPERLLSWHWHPAAIDPAVDYSQEPTTLVVFE
                     LHEGEGGVLLSVIESGLDKIPSHRRATVFRLNSSGWDEQVRNIEKHVAAP"
     gene            complement(1937..2013)
                     /locus_tag="VGE92_05485"
     tRNA            complement(1937..2013)
                     /locus_tag="VGE92_05485"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1977..1979),aa:Pro,seq:tgg)
     gene            complement(2114..2782)
                     /locus_tag="VGE92_05490"
     CDS             complement(2114..2782)
                     /locus_tag="VGE92_05490"
                     /inference="COORDINATES: protein motif:HMM:NF018604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HEY1313303.1"
                     /translation="MKIAITYNESILLFTAFAAGTVDIIGFTTLGGVFASAMTGNFAL
                     LAYYVAQSRSRAAMGSIVALVGFAAGCALGVLQRRGRTQQQALTRLLVGESALLLAFA
                     LYAAASEQVEHSSSNDLLILLLAVAMGLQAVIGQTTNLSTIVFTTTLTKMVGTIADSI
                     ASGDVSALKEVRIPSAVVVSYLFGALLAGALVVRKIDAVVFLPFTGVALAFVMHRLAC
                     RDSA"
     gene            complement(2798..3721)
                     /locus_tag="VGE92_05495"
     CDS             complement(2798..3721)
                     /locus_tag="VGE92_05495"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEY1313304.1"
                     /translation="MLAAGFLASLCRADQPPPVLDEVVVTGERTGPGLWHVHRGDAHV
                     WILGSMSPLPKGITWRSKQVEQILDRTREVLVQKPFEIGIARILWLLITEHNLLMVGG
                     GKRLKDVLPPDLHARFAVQRAKYTTDENKWERFRPLVAAAFLQQDAFHQVGLSTRVDL
                     GAAMRKLAEKHHVALEEIKIAGVRDVIEALKTLPLAAENACVEASLATVESGLPRLVE
                     RAQAWASGNVERIEKLAEPHEVDSCRAALDSGSGAADLIARIRRTWLDAIDARMQSAG
                     VTVAVVNIDMLLEPGGLLDELRAKGYEVDAP"
     gene            3978..4916
                     /gene="yedA"
                     /locus_tag="VGE92_05500"
     CDS             3978..4916
                     /gene="yedA"
                     /locus_tag="VGE92_05500"
                     /inference="COORDINATES: protein motif:HMM:NF008432.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HEY1313305.1"
                     /translation="MSSATQQKFSNRPPTWLIAACLFAVYVIWGTTYFAIKLGIEGAP
                     PFLLIGTRFMAAGGLLLGWQALRGQPMPTARQWRGAAIVGFLLLVVGNGGVAVAEHWV
                     SSGATVALISIMPLGTALWSGAFGDWPRPLEWGAIVLGGIGAAVMLLGADLQASAVGT
                     LVILLGITCWSLGTVLSRRVDVPHGPTGFGAEMLVAGIMALAISAVLGERWALPTAPH
                     FWWVWFYLVIFGSVIAFSAYRFVVERVSPTLAATYAYVNPPVALLVGWWLGHESFSAN
                     ILLGLPIVLGAVAIHAWIQSRPDAAAPALAELDRAA"
     gene            complement(4919..5293)
                     /locus_tag="VGE92_05505"
     CDS             complement(4919..5293)
                     /locus_tag="VGE92_05505"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEY1313306.1"
                     /translation="MNKRGNKKSAVPRFAYDGLDRVIHERARLSVLTSLITHAKGLLF
                     GDLKQLCALTDGNLNRHLQVLEAAKLIAITKGMQNNRPQTYCRITALGRKRYIEYLAV
                     LEQVLLDAAAAVKSDWTAREAT"
     gene            complement(5286..5888)
                     /locus_tag="VGE92_05510"
     CDS             complement(5286..5888)
                     /locus_tag="VGE92_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313307.1"
                     /translation="MKELQEALSQIHSIRTQVASGTEFRGYGPASIAVSGFIALAVAA
                     VQTRWEKPDLTAWVAVWAGTAAVSALLTGIETLRRARREHMGFAREMVQSAVAQFLPA
                     VMVGFLLTAVLMQVAPQDCWMLPGLWQLIFSLGVFASCRFLPRPMFAVGVWYLAAGLL
                     CLILAGATRALSPWMMGIPFGVGQLLVASVLQFGYGESLE"
     gene            complement(6008..>6554)
                     /locus_tag="VGE92_05515"
     CDS             complement(6008..>6554)
                     /locus_tag="VGE92_05515"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEY1313308.1"
                     /translation="IDFSQSSAYVARRLIGMQLLVDGVGGVIVETEAYDREDPASHTF
                     PGPTPRNQVMFGPPGRAYVYLSYGIHWCLNFVCREAGHGAGVLIRALQPTHGVARMME
                     RRRQEDLHLLCSGPGRLGEALGITHELNGKRLDRRPFSLRATGSRVRVIRGPRIGITK
                     AIDQPWRFGLSGSAYLSRPFR"
BASE COUNT         1315 a         2055 c         1910 g         1274 t
ORIGIN      
        1 gtgctgacaa gggacctttg gcgcagaagc tcggcgctca gatctatata gacagcacag
       61 cgcaaaacgc ggccgaagaa ctgaccaagc tcgggggtgc caaagtgatc ctggccaccg
      121 tcactaacgc caagagcatg agcgcggtag tccccggatt gggtatcgac ggcaaactcc
      181 tggtggtggg cgccagcggt gaaccgatcg aggttccgcc cttcttgctc atcggtgctc
      241 ggcgttccat tgcgggttgg ccgtcgggca ctgcaccgga ctccgaagac acgctgaaat
      301 tcagcgtgaa gtcgaacgta cgcccaatga tagagacctt ccccttggcg aaagccgctg
      361 atgcgtatca acacatgatg agcggcaagg cgcgctttcg cgtcgtcttg ataccctagc
      421 gcatagacac atcaggagaa cgccaccatg actcgtcatc tggcatgtcc ccgctcctag
      481 acgcgcgagg ctgtgcccac aaccttgcca catggatgga aggcgacgtt gcccgagtca
      541 cgtcgcgtgt gttcccgcag gaggttagac agtcgttaga tgacgcctcg gacggctccc
      601 gataatatcg tctaagcgat tgatttaatt ggtgggccgt gtagggatcg aacctacgac
      661 caattgatta agagtcaact gctctaccag ctgagctaac ggcccataaa cctttttaaa
      721 tcatcgccct cgccctcgcc ccagctttgc caaagtgggg tggacgacgg ggctcgaacc
      781 cgcgacaacc ggaatcacaa tccggggctc taccagctga gctacgtcca ccataaccaa
      841 agccgtactt cctgcttggg tttggcgcgc ccggcaggaa tcgaacctgc aaccgccggc
      901 ttagaaggcc ggtgctctat ccgattgagc tacgggcgct ttataatcaa taggttacag
      961 cgccaatcgt aatgtttcag gccgacgatc gggtcttaag gcaaaccaaa tcggaagtgc
     1021 gcgcggatta tatagcagtt cttcggtatt tgaagcgccc gatcacaccg gccgagtctc
     1081 agctctcgac cgcgcgcttc agtttgtcga gcgcgtggtc ccactgctgc gcaatccctt
     1141 ccagccagcg ccgtgcctcg tccaattggg cgggctcgaa ctcccacagc cgttcgcgtc
     1201 cgaccttcac gtcacgcacc agaccagcgg cagcgagtac atccaagtgc ttggtgatgg
     1261 cttgtcgggt aatctgcgtg ccggcggtga gttgcgcaat cgacatcacg ccgccgacgc
     1321 acagagtggt catcagccgc agccgcatct cgtcacccaa ggccgcgaat atcggcgccg
     1381 aatcgcgcaa cgacatgttt ctcgatgtca ctttacggcg cggcgacatg cttctcgata
     1441 tttctgacct gctcatccca gcccgaggag ttcagtcgga acacggtcgc acgccggtgg
     1501 gacggaatct tatccagtcc ggactcgatc acgctgagca gcacaccgcc ctccccttcg
     1561 tgaagttcaa aaaccaccag cgtggtcggc tcttgcgaat agtcgacagc aggatcgatg
     1621 gcggccgggt gccagtgcca ggagagcagg cgctcgggaa tcacgcgatc tatcatcact
     1681 tccatcacga tatgttcata gccaggataa gtgatctgcc ccttgacgct ctttccggcc
     1741 tcgaaggact tgcctttgaa atcgacgccg aaccaatcgc caaactcttt ggcgtcggac
     1801 agcgcacgcc aaactcgagc ccgcgtcgcc ctcagtaaga tttttctttc gattcgatct
     1861 gtcgagccca tatgcaacct ccaggatgca tattaagcgc ggcttcggat atatgcaact
     1921 aaagagttgc acttcatggt cggggtgaca ggattcgaac ctgcgacatc tacgtcccaa
     1981 acgtagcgct ctaccagtct gagctacacc ccgaatatcg aagaatgcag cctttcggag
     2041 gcgcgctagt gtacggggtc ggtgggcatt caggcaataa gcgcactgcg gtcgtccgcg
     2101 ccgaatgccc tgactaggca gagtcgcggc acgccagacg gtgcatcaca aacgcgaggg
     2161 caactccggt gaacggcagg aacacgactg cgtctatctt gcgcacgacc agcgcaccgg
     2221 caagcagtgc gccaaaaaga taggaaacaa ccacggcgct cgggatcctc acttctttta
     2281 aggccgagac atcgccgctg gcaatcgaat cggcaatcgt tcccaccatc ttcgtcaagg
     2341 tcgtcgtgaa tacgatggtc gaaaggttcg tagtctgacc tatcaccgcc tgcaacccca
     2401 tcgcgacggc cagcagcaat atcaacaggt cattggagga gctgtgctcg acctgctctg
     2461 aagccgccgc atacagtgcg aacgccaaca ataacgcgct ttcgccgacc agcagccgcg
     2521 tgagggcttg ttgctgtgtg cggccgcggc gttgcaatac gccgagcgcg cagccggccg
     2581 cgaatcccac cagcgccacg atggatccca tggccgcccg cgagcggctc tgcgccacgt
     2641 agtaggccag cagcgcgaag tttccggtca tcgcggacgc aaacacaccg cccaaggtgg
     2701 tgaagccgat gatgtccacc gtcccggccg cgaaggcagt gaacaacagg atcgactcgt
     2761 tataagtaat cgcgatcttc atgccgccga tacgaactca cggcgcatcg acttcatatc
     2821 ctttcgcgcg aagttcatcg agcagccccc caggttctag aagcatgtcg atattgacta
     2881 cggccacggt aactccggcg ctttgcatcc gcgcatcgat ggcatcgagc cacgtgcggc
     2941 gaatgcgggc aatgagatca gcggcgccgg aacccgaatc aagcgctgcg cggcagctgt
     3001 ccacctcgtg aggctcggcc agtttctcaa tgcgctcgac gttgccgctc gcccatgctt
     3061 gcgcgcgctc gaccagacgc ggcaatccgc tctcgacggt cgccagcgag gcctcgacgc
     3121 aggcgttttc ggctgccagt ggcaacgtct tcagtgcttc gatcacatcg cggacgcccg
     3181 cgatcttgat ttcctcgagc gccacgtggt gcttttcagc gagctttcgc atcgctgcac
     3241 ccaagtccac gcgtgtcgat agccccacct ggtgaaaggc gtcctgctgt agaaacgccg
     3301 cggccaccag cggacgaaat cgctcccact tattttcatc ggtggtgtac ttcgctctct
     3361 gcaccgcaaa gcgcgcgtgc aggtccggtg gcaacacatc ttttagacgc ttgccgccgc
     3421 cgaccatcaa aaggttgtgc tcagtaatta acagccacag tatccgagcg atgccgattt
     3481 cgaacggctt ttgcaccaat acttcgcggg ttcggtccaa tatctgctcc acctgcttgg
     3541 agcgccaagt aatgcctttg ggcaacggcg acatgctgcc caggatccac acgtgcgcat
     3601 cgccgcggtg gacatgccac aggccaggtc ccgtgcgttc gccggtcaca accacctcgt
     3661 cgagcaccgg cggcggctga tcggcgcggc acagcgaggc caagaaccct gcggcgagca
     3721 tgccggcccc gagcagcaac cgtgcgcgaa cgaggcaacg agccgctcgc ggcgacttca
     3781 gtctgcgggg atattgcaat ttttaccttc actgttgcag caagccgcgc tgcgttggag
     3841 tggcgcgcat tatcgctaac tttcaatgcg caatcgagca cggtgccggt taagctgcac
     3901 ttaacgcagg tcattataat attgatccgc gccacctgat tatcttggat gatacggcag
     3961 cttgacagga gaaatgcgtg tcgtccgcca ctcagcagaa gttctcgaac aggcccccga
     4021 cttggctcat cgccgcctgt ttgttcgcag tttacgtgat ctggggcacg acttacttcg
     4081 ccatcaagct cggcatcgag ggggcgccgc ccttcttgtt gatcggcacc cgtttcatgg
     4141 cggccggagg attgctgctc ggctggcaag cgctccgcgg tcagcccatg cccacggcga
     4201 ggcaatggcg cggggccgcc atcgtcggtt tcctgttgct ggtggtgggc aatggcggag
     4261 tagcggttgc cgagcactgg gttagctccg gtgccacggt cgcgctcatc agcatcatgc
     4321 cgctcggcac ggcgctttgg tcgggcgcat tcggcgactg gccgcggccc ctggagtggg
     4381 gggcgattgt cctcggtggc atcggcgcgg cggtgatgct gctcggcgcg gatctgcagg
     4441 cgagcgctgt gggaacgctc gtcatcctgc tcggtatcac ttgctggtcg cttggcactg
     4501 tcttgtctcg ccgcgtcgat gttccgcacg ggccgaccgg cttcggagcg gaaatgctcg
     4561 tggccggtat catggcgctc gccatatcag ccgttttagg ggagcgctgg gcgttgccca
     4621 ccgcgccgca cttctggtgg gtgtggttct acttggtcat cttcgggtcg gtgatcgcgt
     4681 tctccgcata ccgcttcgtt gtggaacgcg tttcgcctac gctggcggcg acctatgcgt
     4741 acgtcaaccc gccggtcgca ctgctcgtgg gttggtggct ggggcacgag tcgttctcgg
     4801 cgaatatcct gctcggcttg ccgatcgtgc tcggtgctgt ggcgatacac gcttggatcc
     4861 aatcgcgccc ggacgcggcc gcgccggccc tggcggaact cgaccgagcc gcgtgatatt
     4921 aggtcgcttc cctcgccgtc cagtccgatt tgacggcggc ggcagcgtcc aacagaacct
     4981 gttcgagtac cgccagatac tcgatgtaac gtttacgtcc gagtgcggtg atgcggcagt
     5041 aggtctgggg acgattgttc tgcataccct tggtgattgc gatcaacttc gcggcctcga
     5101 ggacctgcaa atgccggttg aggttgccgt ccgtcaaagc acacagctgt ttcaagtcgc
     5161 cgaacagcaa tcccttcgca tgggtgatga gcgaggtgag cacgctgagg cgcgcacgtt
     5221 cgtgaatcac gcggtcgagt ccatcgtagg cgaagcgcgg aacggcgctc ttcttgttgc
     5281 cacgcttatt caagggattc tccgtaacca aactgcagta ccgacgccac caatagttgg
     5341 ccgacgccga aaggaattcc catcatccaa ggcgacagcg cccgagtggc gccggcgagt
     5401 atcaaacaga gcagtcccgc cgcgagatac cacacgccga ccgcaaacat cggccgcggc
     5461 agaaatcgac aagaagcaaa cactcccaaa ctgaagatca attgccagag ccccggcagc
     5521 atccaacagt cttgcggggc tacttgcatc agaaccgctg tcagcaagaa tccgaccatg
     5581 acggcgggta ggaactgcgc gaccgcggat tgaaccattt cccgcgcgaa acccatgtgc
     5641 tcgcgccggg cgcgccgcaa ggtttcgatg ccggtcagca gcgccgaaac agcagcggta
     5701 ccggcccaca ccgccaccca ggccgtcagg tccggcttct cccagcgcgt ctgcacggcg
     5761 gccactgcga gcgcgatgaa cccgctcacg gcgattgacg ccggaccgta gccgcgaaac
     5821 tccgtgccgc tcgcaacctg ggtgcgaatg gagtggatct gtgacagtgc ttcttgcagt
     5881 tctttcatac aatacaccca ttactttgca ttacaaagtc aaaacgaccg cagtgcgtac
     5941 acaggaaata cctgaaaggc tcatcttggt caaccgacgc ctctgtcggc cgggttggcg
     6001 gccggattca gcgaaacggg cgactcaaat acgcggaccc cgagagtcca aagcgccatg
     6061 gttgatcgat cgccttcgtg ataccgattc gcggtccgcg gatgacgcgg acccgcgatc
     6121 ccgtcgctct caagctgaag ggccgtcggt cgagacgctt gccgttcagc tcgtgagtaa
     6181 tgcctaaagc ctcaccgaga cggcccggcc ccgaacacaa cagatgcaga tcttcttgcc
     6241 ggcgtcgttc catcattcgc gcaacaccgt gcgtcggctg cagcgcgcga atcagcacgc
     6301 ctgcaccgtg tcccgcttcc cggcacacga agttgagaca ccagtggatg ccgtacgaca
     6361 ggtatacata cgcgcgcccg ggagggccga acatcacttg attgcgcggc gtcgggcccg
     6421 ggaaggtatg tgaggcggga tcttcccggt cataagcctc ggtctcaacg atgacgccgc
     6481 ctacaccatc gaccagcagc tgcatgccga tcaagcggcg ggcgacgtag gcggagctct
     6541 gcgagaaatc gatg
//
LOCUS       DASWZT010000203         6542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_35364, whole genome shotgun sequence.
ACCESSION   DASWZT010000203 DASWZT010000000
VERSION     DASWZT010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6542
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35364"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..6185
                     /locus_tag="VGE92_05520"
     CDS             9..6185
                     /locus_tag="VGE92_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY1313309.1"
                     /translation="MGKYRPDASAPAFIYSGNGSQWIGMGLMLLDEDAGFRAAVEEVE
                     ALYACISGQSILTELKAPADQNRFALTEVAQPTLFALQVGLTRMLEHRGIRPIAVCGH
                     SVGEVAAAWASGALTLHQAVRVIHERSTHQAITRGRGCMTVVTLARAETEAMLGTHEF
                     QGHLEIAAVNSPQCVTVAGDATRMARFETVLTQRRIVFQRLALDYAFHSAAMDPIRDN
                     LVQSLHGLKARAIVIPMYSTVTGVLIDTTIAGLDLGPAYWWRNVREPVNFEAAIRAIA
                     ADGVNTFVEIGPRAVLASYLTEITKPLGSAAAVMPSLTRHESGRARVLSLIAQLEITG
                     ALRNASRTAQHPARYVDLPHYPWQRERYWHPTTAESQGQLTRQIEHPLLGYALAGEAL
                     HWENHLDVAKLPTYADHAVGGGAVFPAAGYVEMALAAGRARRRIEQRPEAPPQVIEEL
                     EILAPLLLDADRSRTVRLKIDAADGRFSIVSRERQSAEPWRTHASGRLVEECVVEGAA
                     PLELPVRPEDVGGEAHYAFARTLGLNYGPAFRTVSSVWYRREGLLGTLNTPPEIAADI
                     ATSLLHPAYLDGAFQLLADLALREQRTAGDAPAFLPVRIERLELLQPHEQATCALAEI
                     ADSQRSRRSLRADFTLYDAAGAPIAIARGVRFRAVGIHGGAASRSRWLATRAVPMPRR
                     DSPRAVALPASAALAEVCSLRLHTPKRLAGRKRFSLEVEPLLDALCGSFAARALRELV
                     GNQPIDPQGLVDSGRVAEGSALLLRRLVQILDEDGLVQRFGDQSLWRSDVTLPEHGDI
                     WLSLIADYPEYAALTARVGAAGLHLAERLRSGIGDKLGDGGFAWTEGCTQDEAADVSQ
                     AMLDVIRCTLAAQPAHARLRVLRLVGAAPPAEALPLLPDVDADRCDFVIGAASQTILD
                     DVHGRWPATAALPCMLVDLDRTVQVGGFDLVLLGEGIAGADPERRLANARSLLLDDGR
                     LVLLEQQSSRAADLLFGLHPAWWQGPAADPATLRTRFSRPEEWREMLARANFDRVELV
                     HDLPTAPSGAYLFIARAGVASRNETAPLIESRTWLVLGDAGGYSADLGMALSDVLEAR
                     GQQLVTLVAAASYRPLGPRRYALDPQVAAHWDRLLADLSRSSIALHGWIHLAGIDLAS
                     AAAPPIIRAAAQESRAAVLVSWLQACARRAIHPDVWVVAAQAGTALLPAAVRSALSVD
                     TPGDGLRDAALWGIARVAMQECIDQRIRWVDLIEPVGVTAIAANLGKEILCPDAEDEI
                     LLTASGRFVTRIAPVSSPSLPGASTVLEERRPRVQLDCSVPGPFRNLVWRAAAEPESM
                     AEDEVEIEVHAAGLNFRDVMYAMGLLPDEAVEDGFCGPSLGMEVSGIVTRVGAGVTEV
                     APNDAVIAFAPASFGNRVRTRALAVTRKPAAWSFAAAATVPTAFFTAYYALHELARLR
                     EGERVLIHGAAGGVGIAAIQLAKHLGAEIFATAGTQEKRDFVHLLGADHVFDSRSLAF
                     ADEVLRATGGAGVDVILNSLAGDAIPRNLRLLRPFGRMLELGKRDFYENSQVGLRPFR
                     NNISYFGIDADQLLAQRPDTARRVFVDLMALFLDGTLQPLPHRTFPAMDIAAAFRHMQ
                     ASRHLGKIVVTFPPDFAPLAPGPTEAPPLAIRADATYLVSGGLSGFGLRTARWLVERG
                     ARHLVLLSRRGGAGAPEVAMQLLQFAEQGVMVAAPACDVADFGQLRETLESINTSMPP
                     LAGVVHAAMVIEDALLRDMNRGQLHRVLAPKMLGALNLHQATRACALDWFVLYSSATT
                     LFGNPGQAAYVAANLSMEALANERRSLGLPVTCVGWGPIGDAGYLARNERVLAALVGR
                     MGGEALKSDDLLTALERLIAAPAGNVGFLDLDWKTLCRSLPGAQAPKFSDLARLAARG
                     DRGMHAGDSAQDLRQWLGGLPADEALAELTALVRDEVAGILRIAPERIESTDSLLDMG
                     MDSLMAVELATSIEARLDIRLSALALSGGPTIESIVERIQRLLQPVDAAAEATPNGDL
                     AAQVSLIATQHAGELSAEHAAAMAIEINAASEARSLTRAANG"
     gene            6182..>6542
                     /locus_tag="VGE92_05525"
     CDS             6182..>6542
                     /locus_tag="VGE92_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313310.1"
                     /translation="MSGPRKVLQGLTAPLKERLIQDALQRRLRNVEQERAAPAVTRVS
                     NSTPLRADSIPDSWCRFDLHPDYQQLRILNEGAEKLGVGNPFFRVHEGLAGATTVIGG
                     ETFSNFSSYNYLGLGGHP"
BASE COUNT         1011 a         2208 c         2179 g         1144 t
ORIGIN      
        1 gggtcacggt gggcaagtac cgccccgacg cgtccgcgcc tgccttcatt tactcgggca
       61 acgggtcgca gtggatcggc atgggcttga tgctgctcga tgaggacgcg gggtttcgcg
      121 cggctgtcga ggaagtcgag gcgctgtatg catgcatctc aggccagtcg atcctgacag
      181 agctcaaggc gcccgcggat caaaatcgct tcgcgctcac cgaagtggcg cagccgacgc
      241 tctttgcctt gcaggtgggt ctcacccgca tgctcgagca ccgggggatt cggccgattg
      301 cggtctgcgg ccacagcgtc ggagaagtgg ccgcggcctg ggcgagcggc gcattgactt
      361 tgcaccaggc ggtgcgcgtc attcacgagc gcagcacgca tcaggcaatc acgcgcggca
      421 gagggtgcat gaccgtggtc acgctggcac gtgccgagac ggaagcgatg ctagggaccc
      481 atgagttcca gggccacctc gagatcgcgg cggtcaacag cccgcaatgc gtcaccgttg
      541 ccggcgacgc tacgcgcatg gcgcgtttcg aaactgtact gacgcagcgt cgaatcgtct
      601 tccagcgcct ggccctcgat tatgcctttc acagcgccgc catggacccg attcgcgaca
      661 acctcgtgca gtccttgcat ggcctcaaag cgcgcgccat cgtcatcccg atgtactcga
      721 cggttaccgg cgtgctcatt gacacgacca tcgcgggtct cgacctcggt ccggcctatt
      781 ggtggcgcaa cgttcgcgag ccggtgaatt tcgaggcggc gatccgcgcc atcgcggccg
      841 acggagtcaa caccttcgtc gaaatcggcc cgcgcgccgt gctcgcgagc tacctcaccg
      901 aaatcaccaa gcccttaggg tcggcggccg cggtcatgcc cagtttgact cggcatgaga
      961 gcgggcgtgc gcgcgtgctc agcctcattg cgcaacttga aatcaccggg gcgctgcgca
     1021 acgcctcgcg aaccgcacag cacccggctc gatacgtgga cctgccgcac tatccgtggc
     1081 agcgcgaacg ctactggcac ccgaccaccg ccgaatctca gggtcagctc acacgtcaga
     1141 tcgagcaccc gctgctcggg tacgcgctcg cgggcgaggc gctgcattgg gaaaaccatc
     1201 tggacgtcgc caagctgccc acttatgccg atcatgcggt gggcgggggc gcggtgtttc
     1261 ccgctgccgg ctatgtggaa atggcgctcg ccgccggccg cgcgcgccgc cgtatcgaac
     1321 aacggcctga ggcgcccccg caggtgatcg aggaactcga aatcctggca ccgctgttgc
     1381 tcgacgccga tcgctcgcgc acggtgcgct tgaagatcga tgccgccgac gggcgcttta
     1441 gcatcgtgag ccgcgagcgc cagagcgccg agccttggcg cactcacgcg agcggccggc
     1501 tggtcgagga atgcgtggtc gaaggcgcag cgccgctcga gctgccggta cggccggaag
     1561 atgtcggggg cgaggcccac tatgcgttcg cgcgcaccct cggcctcaat tatggaccgg
     1621 cgtttcgcac ggtttcttcc gtctggtacc ggcgcgaagg gttgctcggc accctcaaca
     1681 cgccgccgga aatcgccgcg gatatcgcaa cctcgctgtt gcatccggcc tatctggatg
     1741 gggcatttca gctgctcgca gatctcgcct tacgcgaaca acgaacggcg ggcgatgcgc
     1801 cggcattcct gccggtccgc atcgaacgcc tcgaactact gcagccgcac gagcaggcga
     1861 cctgcgccct ggcagagatc gcggattcgc agcgcagccg gcgctcgttg cgcgccgatt
     1921 tcactttgta cgatgcggcg ggcgcaccca tcgcgatcgc gcgcggcgtg cgctttcgtg
     1981 ccgtcggcat ccatggcgga gccgcatcga ggtcgcgctg gctcgccacg cgcgccgtgc
     2041 ccatgccgcg ccgcgactcg ccgcgcgccg tcgccctgcc ggcgagtgct gcactcgcgg
     2101 aagtttgctc gctgcggctg cacacgccca agcggctcgc ggggcgcaag cgcttttccc
     2161 tggaagtcga gcctttgctc gatgcactgt gcgggtcgtt cgccgcgcgc gcgctgcgcg
     2221 aactcgtcgg caatcagccc atcgatccgc agggcctggt cgactccggc cgcgttgccg
     2281 agggcagcgc gctgctgctg cgcagattgg tgcagatctt ggacgaggat ggtctggtgc
     2341 agcgcttcgg cgaccaatcg ctgtggcgtt ccgacgtgac gctacccgaa cacggcgata
     2401 tctggctgag tctcattgcc gattacccgg aatatgcggc cctcaccgcg cgcgtgggag
     2461 cggcgggctt gcatcttgcc gagcgcttgc gctcggggat tggcgacaag ctcggggacg
     2521 gcggattcgc gtggaccgag ggctgcacgc aggatgaagc ggccgacgtt tcccaggcga
     2581 tgctcgacgt gatccgctgc accctcgccg cgcagccggc acacgcccgc ttgcgtgtgc
     2641 tgcggctggt cggggcagcg cccccggccg aggctctgcc attactgccc gatgtcgatg
     2701 cggaccgctg cgacttcgtg atcggcgcgg cctcgcagac gatattggac gacgtgcacg
     2761 gccgctggcc ggcaacggct gcgctgccct gtatgttggt ggatcttgat cgcacggtgc
     2821 aagtcggcgg cttcgatctt gtgttgctcg gcgaggggat tgcgggggcg gaccccgaga
     2881 ggcgcctcgc gaacgcgcgc agcctgctgc tcgacgacgg tcggctggta ctgctcgagc
     2941 agcagtcctc gcgcgcagcc gacctgctgt ttggccttca cccggcgtgg tggcaaggcc
     3001 ccgcggccga tccggcgacg ctgcgaacgc gattctcgag accggaagag tggcgtgaga
     3061 tgctggcgcg cgcaaacttc gatcgagtcg agctcgtgca cgacctgccg accgcaccga
     3121 gcggcgctta cctcttcatc gcacgcgccg gcgtagcgtc gcgcaacgag accgctccgc
     3181 tcatcgagag ccgcacctgg cttgtcttgg gcgatgccgg cggctattcc gccgatctcg
     3241 ggatggcgct gagcgacgtg ctcgaggcgc gcggccaaca gctggtgacg ctcgtggccg
     3301 cggcatcgta tcggccgttg gggccgcgac gctacgcgct cgatccgcag gtcgccgcgc
     3361 attgggatcg gttacttgca gatctgtcac gcagcagcat tgcgctgcac ggctggattc
     3421 atttggcagg gattgacctc gcctccgcag cggcgcctcc aataatacgc gctgcggcac
     3481 aggagtcgcg cgccgccgtg ctcgtgtcct ggctccaggc ctgcgcacgg cgcgcaatcc
     3541 acccggacgt gtgggtggtg gccgcgcagg cgggcaccgc actcctgcct gccgccgtcc
     3601 gatccgcctt gtcggttgac acgcccggcg acgggctacg cgacgccgcg ctctggggga
     3661 tcgcgcgggt cgcgatgcaa gagtgcatcg accagcggat tcgttgggtc gacctcatcg
     3721 aaccggtcgg cgtaacggcc atcgccgcga acttaggcaa agagattctg tgccccgacg
     3781 ccgaggatga gatcctgttg accgcatcgg gccgctttgt gacccgcatt gccccggtct
     3841 cctcaccgtc gcttcccggt gcatcgacgg tcctcgaaga acgccgtccc cgcgtgcagc
     3901 ttgattgttc ggtgccggga ccgtttcgta atctcgtctg gcgcgccgcg gcggagccgg
     3961 agtcaatggc cgaggacgaa gtcgagatcg aggtgcacgc ggcgggcttg aactttcgcg
     4021 acgtgatgta tgcgatgggc ttgctgccgg acgaagccgt cgaggatggc ttctgcggtc
     4081 cgagcttggg gatggaagtg tcggggatcg tgacgcgcgt gggcgctggt gtcaccgagg
     4141 tcgcgcccaa tgatgccgtg atcgcgttcg ccccggcatc cttcggcaat cgcgtacgca
     4201 cccgcgcgct cgccgtgacc cgcaaacccg ctgcttggtc gttcgcggcc gcggccacgg
     4261 tgcccaccgc gttcttcacc gcctactatg cgctgcacga gctcgcgcgc ttgcgcgagg
     4321 gtgagcgagt gctcatccac ggcgccgccg gcggcgtggg catcgccgcg atccaactcg
     4381 ccaagcacct cggggcggaa atcttcgcca cggccggcac gcaggagaag cgcgatttcg
     4441 tgcacctgct cggcgccgat cacgtgttcg attcgcgctc gctcgccttc gccgacgagg
     4501 tgctccgggc caccggcggc gccggcgtcg atgtgatctt gaattcgctc gcgggcgatg
     4561 ccatcccgcg aaatcttcgc ctgctgcgcc ctttcggccg catgctcgag ctcggcaagc
     4621 gcgactttta cgagaattca caggtcggct tgcgcccgtt ccgcaacaac atctcgtact
     4681 tcgggatcga tgcggaccaa ctgctggcgc agcgtcccga cacggcgcgc cgcgtgttcg
     4741 tcgatttgat ggccttgttc ctcgacggta cgctgcagcc gctgccgcac cggacctttc
     4801 ctgcgatgga catcgcggcc gcgtttcgcc acatgcaggc atcgcgccac ttgggcaaga
     4861 tcgtcgtcac cttcccgccc gatttcgcac cattggcgcc gggcccgacc gaagcgccgc
     4921 cgctggcgat ccgcgccgat gccacttacc tcgtgagcgg cggcctgtcg ggctttgggc
     4981 tgcgcacggc acgctggctg gtggagcgcg gcgcacggca cctcgtgctc ttgagccgtc
     5041 gcggtggggc cggcgctccg gaggtcgcca tgcagctcct gcagttcgcc gaacagggcg
     5101 tgatggtggc ggcgccggcc tgcgatgtcg cggacttcgg ccaactgcgc gagacattgg
     5161 aatcgatcaa caccagcatg ccgccgctcg ccggcgtcgt gcacgcggcc atggtgatcg
     5221 aggatgcgct gctgcgcgat atgaaccgtg gtcagctaca ccgcgtgctt gcgccgaaga
     5281 tgctgggtgc attgaatctg catcaagcca cacgcgcgtg cgcgctcgac tggttcgtgt
     5341 tgtattcatc ggcaacgaca ctcttcggca atccgggaca ggccgcctac gtcgccgcga
     5401 acttaagtat ggaggcgctc gccaacgaac ggcgcagcct tgggctgccg gtcacctgcg
     5461 tcggctgggg gccgatcggg gatgcgggct atctcgcccg caatgaacgg gtcctcgctg
     5521 cactggtcgg gcggatgggc ggtgaggcgc tgaagtctga cgacctgttg accgccctcg
     5581 agcgcctcat cgcagcgccc gcgggtaatg tgggatttct cgatctcgat tggaagaccc
     5641 tgtgtcgcag cctgccgggt gcgcaggcac caaaattctc ggatctggcg cggctcgcgg
     5701 ccaggggtga tcgcggaatg catgccgggg actccgcgca ggatcttcgc caatggctcg
     5761 ggggtctgcc cgccgacgaa gcgctcgccg aattgacggc cctggtgcgc gacgaagtgg
     5821 ccggcatctt acgcatcgca cccgaacgca tcgaatccac ggactcgctc ctggacatgg
     5881 gaatggactc gctcatggcc gtggaactcg cgacttcgat cgaggcgagg ctcgacattc
     5941 gactatccgc cctcgccttg agcggcggcc cgaccatcga gagcatcgtc gagcgcatcc
     6001 agcgtcttct gcaacccgtc gatgcagccg ccgaagcgac gcccaacggc gacctcgcgg
     6061 cccaggtaag cctcatcgcc acccagcacg ccggtgaact cagcgccgaa catgccgccg
     6121 ccatggcgat cgaaatcaac gccgcatccg aggcgcgttc cctcacgcgg gcggcgaacg
     6181 gatgagcggg ccgcgcaaag tactgcaggg tctgacggca ccgctcaagg aacgtctgat
     6241 tcaggacgcg ctgcaacgac ggctgcgcaa tgtcgaacaa gaacgcgccg cgccggccgt
     6301 gacgcgcgtc agcaatagca cgccgctgcg cgccgactcg attcccgaca gttggtgccg
     6361 cttcgatctg caccccgatt accagcaatt gcgcatcctc aacgaaggcg ccgaaaagct
     6421 cggcgtcggc aatcccttct ttcgcgtaca cgagggtctc gccggtgcga cgacggtgat
     6481 cggcggggaa actttcagca atttttcgag ctacaactat ctcggtctcg gcggtcatcc
     6541 cg
//
LOCUS       DASWZT010000204         4407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_20819, whole genome shotgun sequence.
ACCESSION   DASWZT010000204 DASWZT010000000
VERSION     DASWZT010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20819"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="VGE92_05530"
     CDS             complement(<1..920)
                     /locus_tag="VGE92_05530"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEY1313311.1"
                     /translation="MNNQVHVKFAGKIVFVGFGSIGQGVLPLILRHIGTSSDRITIVT
                     AENSGRGEAEQFAVKFVKTALTQDNYRKVLEPLLHAGDFLLNLSVDVSSVALVQLARE
                     RGALYLDTCIEPWAGGYTDPHASVDSRSNYAMRLQALALRAGAARAPTAVLTHGANPG
                     LVSHLVKQALLNIAKDTGVDAGTPGSREAWAKLAQTLGIKVIHIAERDTQVTNVPKVA
                     NEFVNTWSVDGFVSEGAQPAELGWGTHEKHFPADGGRHTAGSRCAIYLNRPGASTRVR
                     TWTPAAGHFHGFLITHSEAISLADYYTVMEG"
     gene            complement(1028..1693)
                     /locus_tag="VGE92_05535"
     CDS             complement(1028..1693)
                     /locus_tag="VGE92_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY1313312.1"
                     /translation="MALTLYWGSGSPFSWRVLLALEHKRLPYESQLLHFDKQEQQSPQ
                     MLKLNPRGRVPVLKDGDYVVFESVAVLYYLDVKYPQAPIFGANPEEAGVIMRVICEFM
                     AYAEPSLLKITAGIFSGAVAENIDELTDAMHIVAREARTIEGRLSKENWIVGANYSAT
                     DMVIFPWIQVLRRALDRSAAAELGARFLPMERNYPALARWIHRIESLPGYERTYPPHW
                     RET"
     gene            1747..2592
                     /locus_tag="VGE92_05540"
     CDS             1747..2592
                     /locus_tag="VGE92_05540"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEY1313313.1"
                     /translation="MEFQTLIGTDSLQRELGNPALAVIDCRFDLLHPDAGREAYLKAH
                     ILGARYADLNRDLAAAVQAHTGRHPLPAPEAFARRMGALGIGHDSQVVAYDEANGSFA
                     ARLWWLLRWIGHDRVAVLDGGFKAWLARGGEVQSGDTLVPARHLGVRLQPHAVSSSDE
                     VATALKSPDTLLLDARAAERFAGSVEPIDPVAGHIPGAVNHPFSANLEADGRFLPRDE
                     LERRWRARLGSKTPENVIAMCGSGVTACHNLLSLELAGLPGGKLFAGSWSEWIRDPRR
                     PVARG"
     gene            2647..>4407
                     /gene="speA"
                     /locus_tag="VGE92_05545"
     CDS             2647..>4407
                     /gene="speA"
                     /locus_tag="VGE92_05545"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEY1313314.1"
                     /translation="MSTPARSLAANHVWKIEESLDLYQVEAWGKGYFSINEHGHVVIR
                     PSLDAAREIDLLEVVQGLKARDLHTPVVIRFSDILAHRLRHLAEAFATAIAENDYKNR
                     YAAVFPIKVNQQRLVVEEVYRYGKEFGFGLEVGSKPELLAVMSITEDAPDRIVVCNGF
                     KDDTYIEAVILATKLGRNIIPVVENYEEIHLILKHAERYHVRPKIGVRVKLASEGAGR
                     WRESAGEKSKFGLFISEILDLYALLNERDMLDCLQLVHCHPGSQLQDIRRVKDAINEL
                     AHVYAELKLMGAGLQYIDIGGGLGVDYDGSGTNFESSMNYTLNEYASDVVYRIKSVCN
                     ARGVPHPMIVSESGRAIAAHHSVLVFDVLGSSTLDQFKVTGRLEDDYHGTEEVPQPVR
                     DLFDSYRSINERRLVECYHDGLQAREQALQMFNLGYLSLEFRALSEKLYWATCTKIRD
                     FCRKLDRVPEELEDLEDMLSDIYFCNFSVFQSLPDSWAIGQLFPIMPIQRLAERPARN
                     AVLADITCDSDGKIDRFVHPRATKRTLEVHEVRPGEPYYLAAFLVGAYQETLGDLHNL
                     FGDTHVVHIRLREDGGWWIEE"
BASE COUNT          829 a         1387 c         1369 g          822 t
ORIGIN      
        1 ccctccatga ccgtgtagta atccgcgagt gaaatcgctt ccgaatgcgt gatcagaaat
       61 ccatggaaat gaccggctgc gggcgtccag gtgcgaaccc gggtgctcgc tccgggacga
      121 ttcaaataaa tcgcgcagcg cgagccggcg gtgtgccgcc cgccgtccgc cggaaaatgc
      181 ttctcgtgcg tgccccagcc gagctcggcg ggctgcgcgc cttcactgac gaagccgtcg
      241 accgaccagg tattgacgaa ttcgttcgcc actttcggca cattggtcac ctgagtgtcg
      301 cgttccgcaa tgtggatcac cttgatgccg agggtttgcg cgagcttggc ccacgcttcg
      361 cgactgccgg gcgtgccggc gtcgacgccg gtatctttgg cgatgttgag cagcgcctgc
      421 ttcacgagat gcgacaccag gccgggattt gcgccatgcg tcagcaccgc ggtcggcgct
      481 ctcgcggcgc cggcccgcaa tgccagcgcc tgcagacgca tcgcgtagtt gctgcgcgaa
      541 tccacgctgg cgtgcggatc ggtatatccg ccggcccagg gctcgatgca ggtatccaag
      601 tacagcgcgc cgcgttcgcg cgccagttga acgagcgcga cgctcgagac atcgaccgac
      661 aggttcaata gaaaatcacc ggcatgcagc aacggttcca gcactttgcg gtaattatcc
      721 tgggtcaatg ccgtcttgac gaacttcact gcgaattgtt ctgcttcgcc gcgcccgctg
      781 ttctccgcgg tgacgatggt gatgcgatcc gacgaagtgc caatgtgacg caagatcagt
      841 ggcaagacac cttgtccgat gctgccgaag ccgacgaata cgatcttgcc cgcaaatttc
      901 acatgcactt ggttattcat aatttctttc tcatttttgg tttgctcgct caggtgtaga
      961 tggacgccct acgggcggac gccgccagcc tacctggcgg cacccgcaaa atccaagttt
     1021 aggcagctta agtctcgcgc caatgaggcg gataggtgcg ctcgtacccc ggcagcgact
     1081 cgatacggtg gatccagcgc gccagcgcgg ggtagttgcg ctccatcggc agaaaccgcg
     1141 cgccgagttc cgccgcggcg ctgcgatcca aggcacggcg caacacctga atccatggaa
     1201 agatcaccat gtcggtggcc gaatagttgg cgccgacgat ccagttttcc ttgctgaggc
     1261 gcccttcgat ggtgcgcgcc tcgcgcgcca cgatgtgcat ggcgtcggtg agctcgtcga
     1321 tattctcggc gacggcgccg ctgaaaattc ctgcggtgat cttcaggagc gaaggctcgg
     1381 cgtaggccat gaattcgcag atgacgcgca tgatgacgcc ggcctcctcg ggattggcgc
     1441 cgaatatcgg ggcctgcgga tatttcacgt cgaggtagta aagcaccgcc accgattcga
     1501 acaccacgta gtcgccatcc ttgagcaccg gcacgcggcc gcgcggattc aacttgagca
     1561 tctgcggcga ttgctgttcc tgcttgtcga aatgcagcag ctgcgactca tacggcaacc
     1621 gcttgtgctc cagggcgagc aaaacgcgcc acgagaaagg actgccgctg ccccagtaaa
     1681 gagtgagtgc catggcgccg attgtagcgg atgcagttcg cgcggcatcg cgttaagctg
     1741 ggattcatgg aattccagac gctgatcggc acggattcgt tacagcggga gctgggcaat
     1801 cccgcgcttg ccgtcatcga ctgccgtttc gatctgctgc accccgacgc cggacgcgag
     1861 gcctacctta aggcgcatat cctgggagcg aggtatgccg acctcaatcg ggatctggcg
     1921 gcggccgtcc aagcgcacac cggacgccat ccgttgccgg cgccggaggc ctttgcgcgc
     1981 cgtatgggcg cgctcgggat cggccatgac agccaggtcg tcgcctacga cgaggccaat
     2041 ggttcatttg ccgcgcggct ttggtggctg ctgcgctgga tcggtcacga ccgggtggcc
     2101 gtcctcgacg gcggtttcaa ggcgtggctg gcgcgcggcg gtgaggtgca gtcgggggac
     2161 accctggtgc cggcccgcca tttgggcgtg cggctgcagc cgcacgccgt atcgagcagc
     2221 gacgaggtcg cgacagcgct caagtccccg gacaccctgc tgctcgatgc ccgcgccgcc
     2281 gagcgctttg cggggagcgt cgagccgatc gatccggtcg ccgggcacat acccggtgcg
     2341 gtgaaccatc cgtttagcgc gaatctcgag gccgacggcc gcttcctgcc gcgcgacgag
     2401 ctcgagcgcc gctggcgagc gcgcctcggg agcaagacgc ccgagaacgt gatcgcgatg
     2461 tgcggctcgg gcgtgaccgc ctgtcacaat ttgctgagcc tcgagctggc cggcctgccg
     2521 ggcggaaaac tgtttgcggg atcttggagc gagtggatcc gcgatccgcg ccgacccgtc
     2581 gcccgtggct agagctgtgg tttgcgtcat gtacatgcag cggcaatttg ttatcgtgcg
     2641 cgcgacatga gcacacccgc cagatcgcta gccgcgaacc acgtttggaa aatcgaagaa
     2701 tcgctcgacc tgtatcaagt cgaggcctgg ggcaagggct acttttcgat caacgagcac
     2761 ggtcatgtcg tgatccggcc gagcctcgac gcggcgcgcg agatcgacct tctcgaggtc
     2821 gtccaaggcc tgaaagcccg cgatctgcac acgccggtgg tcattcgctt ctccgacatc
     2881 ctggcgcacc ggctgcgcca cctggccgag gcatttgcca ccgccatcgc cgaaaacgac
     2941 tacaagaacc gctacgccgc ggtgttcccc atcaaggtca atcagcagcg cctggtcgtc
     3001 gaagaggtct atcggtacgg caaggaattc ggcttcggcc tcgaggtcgg gtccaaaccc
     3061 gaactgctcg cggtcatgtc catcaccgag gacgcgccgg accgcatcgt ggtgtgcaac
     3121 ggcttcaagg acgataccta catcgaggcg gtgattctcg ccaccaagct cggtcgcaac
     3181 atcatcccgg tagtcgagaa ctacgaagag attcatctga ttctaaagca cgccgaacgc
     3241 tatcacgtgc ggccgaagat cggcgtacgc gtcaagcttg ccagcgaggg cgccggccgg
     3301 tggcgcgaat ccgccggcga gaaatccaag ttcggcctgt ttatcagcga gatcctcgac
     3361 ctgtacgcgc tgctcaacga gcgcgacatg ctcgattgtc tgcagctcgt gcactgccac
     3421 ccgggcagcc agctgcagga catccgccgc gtcaaggatg ccatcaacga actggcgcat
     3481 gtctacgcag aactcaagct catgggtgcc ggcctgcaat acatcgacat cggcgggggc
     3541 ctcggcgtcg actacgacgg cagcggcacc aatttcgaat cgtccatgaa ctacacgctc
     3601 aatgaatacg cgagcgatgt ggtgtaccgc atcaagagcg tctgcaacgc acgcggcgtg
     3661 ccgcatccga tgatcgtcag cgagtccgga cgcgccatcg ccgcgcacca cagcgtgttg
     3721 gtgttcgacg tactcggctc ctcgacgctc gaccagttca aggtcaccgg ccgtctcgaa
     3781 gacgactacc acggcaccga ggaagtgccg cagccggtac gcgatctttt cgacagctac
     3841 cggtcgatca acgagcggcg gctggtggag tgctatcacg acggcttgca agcgcgcgaa
     3901 caggcgctgc agatgtttaa cctgggctat ttgagtctgg agtttcgcgc cctgtcagaa
     3961 aaactctact gggctacctg caccaagatt cgcgactttt gccgcaaact cgaccgggtt
     4021 ccggaagaac tggaagacct ggaagacatg ctgagcgaca tctatttctg caacttctcg
     4081 gtattccaat cgctgccgga cagttgggcg atcggccagt tattccccat tatgccgatc
     4141 cagcgtctgg cggagcggcc ggcccgcaat gccgtgctcg ccgacatcac ctgcgactcg
     4201 gacgggaaaa tcgaccgctt cgtgcacccc agagcaacta agcgaaccct ggaagtccac
     4261 gaggtcaggc ccggggagcc ctactacctc gctgccttct tggtgggcgc ctatcaggag
     4321 accctgggcg acctgcacaa tctcttcggt gacacccatg tggtgcatat tcggctgcgg
     4381 gaggacggcg gctggtggat cgaggag
//
LOCUS       DASWZT010000205         8180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_35365, whole genome shotgun sequence.
ACCESSION   DASWZT010000205 DASWZT010000000
VERSION     DASWZT010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8180
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35365"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /locus_tag="VGE92_05550"
     CDS             complement(<1..1174)
                     /locus_tag="VGE92_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313315.1"
                     /translation="MSPPDIIVTEPSIAVPIGDVGLAIEQPRVFGLRAWLLTAFLFLV
                     PMVTYWPATFHDFGLRDDYSNLREAHEEPGTVIKFCASHARPIYGWLLQATYGQTGTV
                     QNLQWMRFAASLLLGALSLVSFRGLRALGWSFSSSLSVAVLLALIPSAQVIAGWAIGW
                     PYAAAALLSVGAFFTVEGALVVGTSAGPARAVGQWLVALGLLVVSALIYQPSALFYVV
                     PLAAAVIVQRHRNLVQSARWSGIHAGFVVAALGLAYSVMSVLYATGVFVKSGRIAFEH
                     HWGEKIAWFLQECLPNGLSLFVLNDNNHRDHALYFGCAAVVGALLLAGAYLEWRRYGH
                     SRGIVWLAALLGLPVFACAVSLIASEHYATYRTILAMTAVLLCFLVASVRALTESIG"
     gene            complement(1381..2718)
                     /locus_tag="VGE92_05555"
     CDS             complement(1381..2718)
                     /locus_tag="VGE92_05555"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY1313316.1"
                     /translation="MSEAAEWKQKYRDSLLEMEAEEKRWRDIEYALRRLVGRLCAAGM
                     GVNPQLDDELTAVAAANRRNADVLELEKLAASLTTTVVAVDAVAPVVSVQAAPLRWHA
                     TCGAVAAFLEQLRADGTDESRVQALLSELLAAAGDAALAAVVSGTADLVRARSAALAA
                     ERARAAQVLSEVTKRLEEVAGYLTESNSASRARFDDAQSLNDTVLSHVRELNEEVSGA
                     TELGRLQSIVRSRLEAVAKQVCDFRARETDRLLESNGRADRMRARIADLERETQELHS
                     KLDLEKHGARLDPLTGVANRKSYDERFAQDIARRDAGGAPLALLLWDIDNFKLINDNY
                     GHRAGDRVLQSVAACFKAALRSSDFVARIGGEEFAVLLNGLKLAEAVNVANQLRTAVE
                     GLRFHFRGTPVRVTVSCGVSQLNPGDSPEASYERADQALYRAKGGGKNLCVAA"
     gene            2898..3527
                     /locus_tag="VGE92_05560"
     CDS             2898..3527
                     /locus_tag="VGE92_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY1313317.1"
                     /translation="MVLKPAFLIATLLIASTAAASPHIYTYVDDDGVRHYTDAPDNNR
                     YRLLILSPRERTVSGDRYDALLLAKASQYDSIIETAATAASVEPNLLRAVIVVESGFN
                     SHAVSKRGAVGLMQLMPATATRFGVSNPYDPRENVHAGARYLKFLMDRFGQDVRLALA
                     AYNAGEEAVERNGGQIPPFSETLAYVPRVLKIYRMLREQPQPHATMVSG"
     gene            complement(3530..4060)
                     /locus_tag="VGE92_05565"
     CDS             complement(3530..4060)
                     /locus_tag="VGE92_05565"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1313318.1"
                     /translation="MKTPSVLAIGAVSLLFGIGIGFAAKITGAGIAVIRDKPAKEAAL
                     AALAEAERVADNGSWELIAVGRVYFLSGDKARGQALFDHVLGGKPGGADYEKIASVYA
                     EAGDNDQATANYEKMLAIDPKDDSSQAEVGAWYLRIGQRAKGETLLAQALQRRPNTPW
                     YYVRAAESLLGVPPGQ"
     gene            4163..4474
                     /locus_tag="VGE92_05570"
     CDS             4163..4474
                     /locus_tag="VGE92_05570"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY1313319.1"
                     /translation="MAYQDALLALADPTRRRVFEELRRGPKSVGRIAARMPVSRPAVS
                     QHLGVLKQAGLVVDRAEGTRRVYRIDPAGLAEIRNWLDQFWDVALAAFQAELEKKEDD
                     T"
     gene            4471..4950
                     /locus_tag="VGE92_05575"
     CDS             4471..4950
                     /locus_tag="VGE92_05575"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY1313320.1"
                     /translation="MTLIASVRKQLRVQASQSRAFQVFTEHMSRWWPATHSILKSPLK
                     ECIIEPRVGGRWYAVGEDGSTCQTGYVIGWQPPQTLLLAWQINAAWQYDPALVTEVEV
                     RFIAEGADTTRIELEHRHLERMGDMAAAARSAMDSPRGWSAILESFRTFAQTTNSEG"
     gene            4953..5483
                     /locus_tag="VGE92_05580"
     CDS             4953..5483
                     /locus_tag="VGE92_05580"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY1313321.1"
                     /translation="MNNMQLIICTAVACAAMSEAAAEVLSVAGNGFEVRETAHIAAQP
                     DKVFAALLVPAHWWNSEHTFSGSAANLVLDARAGGCFCETLPGGGSADHLTVVFVSPG
                     KTLRLRGALGPFQGLAVDGAMTWSVKSAAGGTDLSLTYSIGGYLKNGFDELSKAADQV
                     LGDQVMRLKKSLEPAT"
     gene            complement(5455..6090)
                     /locus_tag="VGE92_05585"
     CDS             complement(5455..6090)
                     /locus_tag="VGE92_05585"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY1313322.1"
                     /translation="MATSSKRGRPLHDDVLTPAEWRVVHAVKHGMTSREIAARRGISI
                     DAVKYHVANALAKLGLANRRALRHWHQPPKRSALTRQETPVNAATALGPIGQIARSVR
                     NIEEAASWYGSVLGIPHLYTFGKLAFFDCGGTRLMLTEEAEVRAAESILYLRVPEIAA
                     AHASLESRGVEFINAPHMVHKHADGTEEWMAFFKDPEGRTLALMSQARAIS"
     gene            6210..7100
                     /locus_tag="VGE92_05590"
     CDS             6210..7100
                     /locus_tag="VGE92_05590"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY1313323.1"
                     /translation="MSDAESAQSSAVAFRGTRALCAEFSLMLEARGIDHEVRADEGSW
                     VVTVPPPLAQRAYDEIERYCAERNVPRSRPARIELFPGAAFGAVGYGLILLFTAYCAG
                     IDLFGVDWYSAGALDAGATREWWRALTALTLHVDQEHLLGNVLFGVLAGIAAGRLLGP
                     GIAWASIIAAAALANYTEMLIAPLDHHAVGASTAVFAALGLLSGLAWRQRHKRERRWY
                     RWAPLIAGISLLTLLGAGNAHVDVLGHALGFVFGVGAGSLYAYSRRPRNRGSRLQIAS
                     GSGAVLLIGLAWLLALGHAG"
     gene            complement(7117..7449)
                     /locus_tag="VGE92_05595"
     CDS             complement(7117..7449)
                     /locus_tag="VGE92_05595"
                     /inference="COORDINATES: protein motif:HMM:NF039485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HEY1313324.1"
                     /translation="MGMLDGLLGGVVGGEMATVVNGLIEKHGGVQGIVAQLEQQGLGN
                     TVRSWVGNGANQPITADQVHQAFGSDTVKALAAKVGLTPEDLTAKLSQILPGVIDKLT
                     PAGTVAKT"
     gene            complement(7555..7956)
                     /gene="mscL"
                     /locus_tag="VGE92_05600"
     CDS             complement(7555..7956)
                     /gene="mscL"
                     /locus_tag="VGE92_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003655263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEY1313325.1"
                     /translation="MGLGAEFKEFAMKGNVVDLAVGVIIGAAFGKIVSSLVGDIFMPP
                     LGRFLGGVNFSDMAVSLGKDPAGKDVLVRYGAFAQTIFDFIIIALVLFFVIKAINKLK
                     KPPPVVAPPPAPPAKSELLLEEIRDLLAKRA"
BASE COUNT         1522 a         2554 c         2578 g         1526 t
ORIGIN      
        1 gtccgatgct ctccgtcagt gcgcgaaccg aggccaccag aaaacacagc aacacggcag
       61 tcattgccaa aatcgtgcga taggtggcgt agtgttccga ggcgatcaag ctcaccgcac
      121 acgcgaacac cggcagaccc agaagcgcgg ccagccacac gatgccgcgc gaatgtccgt
      181 agcggcgcca ttcgaggtac gcgcccgcca gcagcagtgc gccgaccacc gcggcacagc
      241 cgaagtacag cgcatggtcc cgatgattgt tgtcgttcag cacgaacagg ctgaggccgt
      301 tgggcaggca ttcctgcagg aaccacgcga ttttctcgcc ccaatgatgc tcgaaggcaa
      361 tgcgcccgga tttgacgaaa actccggtcg cgtacagcac gctcatcacg gagtacgcga
      421 ggcccaaagc cgcgaccacg aagcctgcgt gaattccgct ccagcgcgcg ctttgcacca
      481 agttgcgatg ccgttgcacg atcacagcag ccgccagagg cacgacatag aacaacgcgc
      541 tcggctggta gatgagcgcg ctcacgacca gaagacccag ggccaccagc cattggccga
      601 cggcacgggc cggacctgca ctcgttccga cgaccagagc gccttcgacg gtgaagaagg
      661 cgccaacgga caaaagggcc gccgcagcat acggccagcc gatcgcccat ccggcaatca
      721 cctgcgcgga cggaatcaat gcaagcagta cggccacact caagctgctg ctgaacgacc
      781 agccgagcgc gcgcaggccg cgaaaactaa ccaacgacag cgcccccaac aacaacgagg
      841 ccgcaaagcg catccattgt agattctgga ccgtgccggt ctgcccatag gtggcctgca
      901 gcagccagcc gtagattggg cgtgcgtgcg atgcgcagaa cttgatgacc gtgccgggct
      961 cctcgtgcgc ctcgcgcaaa ttggagtagt cgtcccgcaa gccgaagtca tgaaaggtcg
     1021 ccggccaata cgtcaccatc ggcaccagga aaaggaacgc ggtgagcagc cacgcacgca
     1081 gaccgaaaac ccggggctgt tcgattgcaa ggccgacatc gccaatcggg acggcaatcg
     1141 agggctcggt aacgatgata tcgggagggg acataggaac tctaagaggg tctcctcatg
     1201 ttgcaatgca cagtcaactt gacgccggta ctcggttgcc ggtggcaacg aaagtatcgc
     1261 tgtagtgggg agtactggcg cggaatcgat tttagccaca cgtggcggca aaaatccgcg
     1321 agaaaagccc agaaaattcg ttaaatatct gttagctaag caaaatcgcc aggcgggcgg
     1381 ctaggcggcg acgcacaggt tcttgccgcc gcccttggcc cgatacaggg cttggtcggc
     1441 gcgctcgtac gaggcttcgg gcgagtcgcc cggattcagt tggctgacgc cgcatgacac
     1501 cgtgacgcgc accggcgtgc cgcggaaatg gaatcgcagt ccctcgaccg cggttcgcaa
     1561 ctggttcgcg acgttgacag cctcggcgag cttcaggccg ttcaacagca ccgcaaattc
     1621 ctcaccgcca atgcgcgcca cgaagtcgct cgagcgcagc gccgctttga agcaggcggc
     1681 gacgctttgc aggacgcggt ctcccgcccg gtgtccgtag ttgtcattga tcagcttgaa
     1741 gttgtcgatg tcccacagca gcagagccag cggcgcaccg cccgcatcgc gccgcgcgat
     1801 gtcctgcgcg aaccgctcat cgtaggattt gcggttggcg acaccggtca agggatcgag
     1861 tcgcgccccg tgcttctcca aatcgagttt ggagtgcagt tcctgcgtct cgcgctcgag
     1921 gtccgcgatg cgagcgcgca tgcgatcggc gcggccattc gattccaaca ggcggtccgt
     1981 ctcgcgagcg cgaaagtcgc agacctgctt ggcgaccgct tccaggcgcg acctgacgat
     2041 ggattgcaac cggccgagtt cagtggctcc gctcacctct tcgttgagct cccggacatg
     2101 cgacagcacc gtgtcgttga gcgattgggc atcgtcgaac cgagcgcggc tcgcgctgtt
     2161 cgattcggtc aggtagcccg ccacttcctc gaggcgcttg gtcacctccg acagcacttg
     2221 cgcggcgcgc gctcgttcgg ccgccaaggc ggcgctgcgc gcgcgaacca gatccgcggt
     2281 gccgctcacc accgcggcca acgcggcgtc gccggcggcc gccaggagct cggagagcaa
     2341 agcctgcact ctcgattcgt cggtcccgtc cgcgcgaagt tgttcgagaa acgccgccac
     2401 ggcgccacag gtcgcatgcc agcgcaatgg cgcggcttgg acactcacga ccggcgcgac
     2461 ggcgtcgacg gccacgaccg tcgtggtcaa ggaggcagcc agtttttcga gttcgagtac
     2521 gtctgcgttg cgtcgattgg cggccgcgac tgcggtcaac tcgtcgtcaa gttgcggatt
     2581 cacacccatg cccgccgcgc acagacggcc gacgagccgg cgcagggcgt actcgatgtc
     2641 gcgccagcgt ttctcctcgg cctccatttc caggagcgag tcgcgatatt tttgtttcca
     2701 ttcagcggct tcggacatcg ggttggcttt gtttaatggg taaggatatc ggtgccaatc
     2761 gatgaagctt tagctcgctc ccccagaccc ggctttatta gatgcagcgc acagtgagca
     2821 gcgagcattt cttgatgcag gtccgaccgt ctgcgcacgc gccgcgatag gctttcgccg
     2881 aaggacgcta cacattcatg gtgctcaagc ccgcattttt gatcgcaact ctgctgatag
     2941 cttcgaccgc ggcggcaagt ccgcatattt acacctacgt tgacgatgac ggcgtgcgcc
     3001 attacaccga tgcgccggac aacaaccgct accgtctact gatcttgtcg ccccgggaga
     3061 ggacggtgag cggcgatcgc tacgatgcgt tgctgctggc aaaagccagt cagtacgact
     3121 cgatcatcga gacggccgcg actgcggcat cggttgaacc caatctgctg cgcgccgtga
     3181 tcgtcgtcga atcgggcttc aattcgcatg ccgtctcgaa gcgcggcgcg gtcgggctga
     3241 tgcagttgat gccggccacg gcgactcgct tcggagtttc caatccctac gatccacgcg
     3301 aaaatgtgca tgcaggggcg cgctatttga agtttctgat ggaccgcttc ggccaggatg
     3361 tgaggctggc gcttgccgcg tataacgcgg gcgaggaggc ggtcgagcgc aacggcggtc
     3421 aaatcccgcc gtttagcgag actctggcct atgtgccgcg agtcctgaaa atataccgca
     3481 tgctgcgcga gcaaccgcag ccgcacgcaa cgatggtatc gggctagcgt tactggccgg
     3541 gcggaacgcc gagcaggctc tccgcggcgc gcacgtaata ccacggggta ttcggacggc
     3601 gttgcagcgc ctgcgccaac aaagtctcgc ctttggcgcg ctggccgatt cgcaggtacc
     3661 aggcacccac ctcggcttgg ctcgaatcat ccttgggatc gatcgccagc atcttctcgt
     3721 agttagcggt cgcttgatcg ttgtcgccgg cttcggcgta aacactggca atcttctcgt
     3781 agtcggcgcc gccgggtttg ccgccgagca catgatcgaa cagtgcctgg ccgcgcgcct
     3841 tatcgcctga cagaaaatag acgcggccca cggcaatcag ctcccacgag ccgttgtccg
     3901 cgacgcgctc tgcctcggcc aaggcggcga gcgcggcttc cttggcgggc ttgtcgcgaa
     3961 tcaccgcaat gccggcaccg gtgattttgg cggcaaatcc aatgccgata ccgaacagca
     4021 gactgaccgc gccgatcgcg agcaccgagg gagttttcat aggttggagc ccttgttgtt
     4081 gttgaatccc gcccgtcatc gtaatcaagg acaagtcgac ctgcatggtt gactgatggt
     4141 tagtattcgc ttactgttag tcatggctta ccaagatgcg ctgctcgctc tcgctgaccc
     4201 gacccggcgg cgagtgttcg aggaattgcg tcgcggaccc aagtcggtgg gcaggatcgc
     4261 ggcgcgcatg ccggtgagca ggccggcggt ctcccagcat ttgggggttc tgaagcaggc
     4321 gggattggtc gtcgaccggg cggaggggac gcgccgggtt tatcggatcg acccggcggg
     4381 gctcgccgaa atccgcaatt ggctggatca gttctgggat gtggcactgg ccgctttcca
     4441 ggcagagttg gaaaaaaagg aggacgacac atgaccttga tcgcatcggt gcgcaagcaa
     4501 ttgcgagtgc aggcgagcca gtctcgcgcc tttcaagtgt ttaccgaaca catgtcccgg
     4561 tggtggccgg ccacgcactc gatcctcaag tcgccgctca aagagtgcat catcgaaccg
     4621 cgcgtgggcg gccgctggta cgccgtcggc gaggacggct ctacctgcca gacgggttac
     4681 gtgatcggct ggcaaccgcc gcaaactctg ttgttggcgt ggcagatcaa cgccgcgtgg
     4741 cagtacgatc cggctctcgt caccgaggtc gaagtgcgct tcatcgccga gggtgccgac
     4801 acgacgcgaa tagagttgga gcatcgtcat ttggaacgaa tgggggacat ggccgccgcc
     4861 gcgcgcagcg cgatggattc gccgcgtgga tggagcgcga ttctcgaatc tttcagaaca
     4921 ttcgcgcaaa caacaaacag cgagggatga tcatgaacaa catgcagctg ataatttgca
     4981 ctgccgtggc ctgcgccgcg atgtccgagg ccgcggccga ggtcctgagt gtggccggca
     5041 acggctttga agtgcgggag accgcgcaca tcgcggcgca gcccgacaaa gtgttcgcag
     5101 cattgttggt gccggcgcac tggtggaatt ccgagcacac attttcggga agcgcggcga
     5161 atctcgtatt ggatgcgcgc gccgggggtt gcttctgcga aactttgccg ggcggcggct
     5221 cggccgacca tttgaccgtc gtgttcgtct cgccgggcaa gacgctgcgc ctgcgcggcg
     5281 cgctcgggcc tttccaagga ctcgccgtcg acggcgccat gacctggagc gtgaagagcg
     5341 cggccggcgg cacggatctt tcgctgacct attcgatcgg cggctacctc aagaacggct
     5401 tcgatgaatt gtcgaaggcg gccgatcaag tcctcggcga tcaggtcatg cggctcaaga
     5461 aatcgctcga gcctgcgaca tgagcgccag cgttcggccc tcgggatcct tgaagaacgc
     5521 catccattcc tcggtcccgt ccgcatgctt gtgaaccata tgcggcgcat tgatgaattc
     5581 gacgccgcgc gattccaggc tggcatgtgc cgccgcgatt tccggaacgc gtaaatagag
     5641 aatggattcc gcggcgcgca cctcggcttc ttccgtcagc atgaggcggg taccgccgca
     5701 gtcgaagaat gcgagcttgc cgaacgtgta gagatgcggg atgccgagca cgctgccgta
     5761 ccagcttgcc gcttcttcga tgtttctgac cgagcgcgct atttgtccaa tcggacccaa
     5821 ggcggttgct gcattcacgg gagtctcctg ccgggtaagg gcgctgcgtt tgggtggctg
     5881 gtgccagtgg cgcaaggcgc ggcgattggc gagtcccagt ttcgcgagcg cattcgcaac
     5941 gtgatatttg accgcatcga tgctgattcc gcggcgcgcg gcaatctcgc ggctggtcat
     6001 accgtgcttg acggcatgca ccacccgcca ctcggcgggc gtcaaaacgt catcgtgcaa
     6061 gggtcggccg cgtttcgatg aggtagccat ggggcgatca taatcttccg tgctccgatt
     6121 taccctcccc acgcggctcg cgtgctgcgg gtcgcgattc tcaactgacg ccgttgattt
     6181 gcacggcgcc ggccccattg ggtctggcca tgagcgatgc agaatctgcg caaagttcgg
     6241 ccgtggcgtt tcgcggtacg agggcgctgt gcgcggaatt cagcctgatg ctcgaagcgc
     6301 gcggcataga ccacgaggtg cgcgcggacg agggctcctg ggtcgtgacc gtgccgccgc
     6361 cgttggcgca gcgggcgtac gatgagatcg agcgctattg cgccgaacgc aacgtgccgc
     6421 gaagccggcc ggcgcgcatc gaattgtttc ccggcgcggc gtttggcgcg gtcggctacg
     6481 ggttgatcct gttgttcacg gcgtactgcg cgggcatcga cttgttcggc gttgattggt
     6541 attccgcggg cgcgctcgac gccggtgcaa cccgtgaatg gtggcgcgcg ctgacggcgc
     6601 tcacgttgca cgtggaccag gagcatctgc tcggcaatgt gttattcggc gtgctggcgg
     6661 gaatcgccgc cggccgtctg ctcggtccgg gcatcgcctg ggcaagcatc atcgctgcag
     6721 ccgcactcgc caattacacg gaaatgctca tcgcccccct cgatcatcac gcggtcggcg
     6781 cgtcgaccgc tgtgttcgct gcgctcggct tgctcagcgg gctggcgtgg cggcagcggc
     6841 acaagcgcga gcgccgctgg taccggtggg cgccgctgat cgccgggatc tcgctgctga
     6901 ctctgctcgg cgccggcaat gcccatgtcg atgtgctcgg gcacgcgctc ggattcgtgt
     6961 tcggcgtcgg cgcgggttcg ctgtatgcct attcgcgccg gccgcgcaac cgcggctcgc
     7021 gcctgcaaat cgcgagcggt agcggcgccg tgctgctcat cgggttggcg tggctcttgg
     7081 cgctcggcca cgcgggctag ccccaagcag cggcgctcag gtcttcgcga ccgtacccgc
     7141 gggcgtgagt ttgtcgatca ctcccggcaa aatctgcgac aacttcgcag tcaaatcttc
     7201 cggcgtcagg ccaaccttcg cggcgagcgc ctttacggta tcggagccga acgcctgatg
     7261 cacctggtcg gcggtgatcg gctgattggc gccgttaccg acccacgagc gaacggtgtt
     7321 accgagcccc tgttgctcca gttgcgcaac aatgccttga acgccgccat gcttctcgat
     7381 gaggccattg accacggtgg ccatttcgcc gcccaccacg ccacccagta aaccgtcgag
     7441 cattcccatc ttgagcaacc ccttagaatt cggaaaataa gattcgcttc agaaaattgg
     7501 cgcctgaagg ccgcacgccg tcgctgcggc gtgcgtcccc ggccgatact gcaactaggc
     7561 gcgtttcgcg agcaggtcgc gaatctcctc gaggagcaac tccgattttg ccggcggtgc
     7621 gggcggtggc gccacgacag gaggcggctt cttgagttta ttgatggcct tgatcacgaa
     7681 gaacagcacc aacgcgataa tgatgaaatc aaaaatggtc tgggcgaaag ccccgtacct
     7741 gaccagcacg tctttgccgg cggggtcctt gccgaggctc accgccatat ccgagaagtt
     7801 gacgccgccg agaaatctgc cgagcggagg catgaatata tcgccgacca gcgaggagac
     7861 gatcttgccg aaggcagcgc cgatgatcac accgaccgcc aaatcgacca cattgccctt
     7921 catcgcaaat tctttgaatt ctgcacctaa tcccacggca ttctccttgt tttacggacc
     7981 gacatttcag cgttcacttc ctcgcaacgg ggcatggtac tcagcgcatc cccgaggggc
     8041 aagtgatgtg cggactagtg cagggcgccg aagactttct tcagcaaatt gctggcctgt
     8101 ccgagcgggt ctttgcgaat ggcgcgctcc tcctccgcca tcatcaagaa tagaccgtcg
     8161 agcgttttgt cggtcacata
//
LOCUS       DASWZT010000206         1856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_40363, whole genome shotgun sequence.
ACCESSION   DASWZT010000206 DASWZT010000000
VERSION     DASWZT010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40363"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VGE92_05605"
     CDS             complement(<1..330)
                     /locus_tag="VGE92_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313326.1"
                     /translation="MPLLDIILPDTDAPVVKWGKLYGAAAALAIAEAAAHSHAPLIVL
                     AHNSREAEALGDELRFFAAAELPVRVFPDLETLPYDAFSAHPDITSARLATLAELPRA
                     RRGVWLVA"
     gene            complement(412..687)
                     /locus_tag="VGE92_05610"
     CDS             complement(412..687)
                     /locus_tag="VGE92_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313327.1"
                     /translation="MRYAILMLAAVLAGGSACAETVAVDNGIAVKESDVATPTRGMSM
                     DQVTSKFGAPANKVPAVGKPPISRWEYPGFIVYFEADHVIHSVVANS"
     gene            816..>1856
                     /locus_tag="VGE92_05615"
     CDS             816..>1856
                     /locus_tag="VGE92_05615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEY1313328.1"
                     /translation="MFQPLPVFVGLRYSLAREHSFFVSFITWVSLAGVALGVAALITV
                     LSVMNGLESELRTRLLSLTAHASLNAKGAPIRDWPTVIRRLQGSPGLVGAAPFLDTDA
                     MLSRPPSMSGAIVRGIDPQLETQVSSIADAMREGKLSDLEPGTNRIILGRMLAYQLQV
                     GVGDAVTIMIPGNTASAGGANDGIVPRLRDFQVAGIFEVGLQEHDSVLALVNLQDSEA
                     LRGIDGATGIRLKFTDVLQAPELARIAASRLAPGLQVRDWTQDNEAYFRAIRIEKTMM
                     GLILMLIVAVAVFNIVATLIMVVNDKRTDIAILRTLGLSPRGVLAVFMTQGLLIGWIG
                     AAAGVALGLALAL"
BASE COUNT          327 a          579 c          587 g          363 t
ORIGIN      
        1 cgccaccagc cacacgccgc gacgcgcccg cggcaattcg gcgagcgtgg cgaggcgcgc
       61 ggaagtgata tcgggatgcg cggaaaaggc gtcgtagggt agggtctcca aatcgggaaa
      121 cactctgacc ggcaactcgg ctgctgcgaa aaagcgcaac tcatcgccga gcgcttcggc
      181 ctcgcgcgag ttgtgcgcga ggacgatcag cggcgcatgc gaatgcgccg ccgcttcggc
      241 aattgcgagg gcggcggccg cgccatagag cttgccccac ttgacgacgg gcgcatcggt
      301 gtcgggcagg ataatgtcga gcagtggcat ctcacgaacg caaacaaata acgcccgccg
      361 cgaacaggtc gcggtgggct tgcaggactt gagatcttac gcgatttcgc gctatgagtt
      421 tgcgaccacg ctgtggatca catgatcggc ttcgaaatag acgatgaacc cgggatactc
      481 ccagcgcgaa atcggcggct tgccgaccgc cggtacttta tttgccggcg cgccgaattt
      541 gctggttacc tggtccatac tcatgccgcg tgtcggcgtg gcgacatcgg attctttgac
      601 cgcaattccg ttgtccaccg ccacagtctc ggcgcaggcc gagccgccgg cgagcaccgc
      661 cgccagcatc aggattgcat acctcatggt gcacctcatt tatttcccct cgagcaaact
      721 cggttcctcg taaactataa caccacacgc gcaggcaata ctttgactca attaacactc
      781 tgattatatt gttatttgtg gtttaatcgc cgcgcatgtt tcagcccctt ccagttttcg
      841 tgggcctgcg ctattcgctg gcccgcgaac acagcttttt cgtgtctttc atcacctggg
      901 tctcgctcgc cggcgtggcg ctgggtgtcg ccgcgctgat caccgtgctg tcggtgatga
      961 acggactcga gtccgaattg cggacccgcc ttttgagcct caccgcgcac gcgagtctca
     1021 acgccaaggg cgcgccgatt cgtgattggc cgacggtgat tcgccgactg cagggctcgc
     1081 cgggtctggt gggtgcggcg cccttcctcg ataccgatgc gatgttgagc cggccgccgt
     1141 cgatgagcgg cgcgatcgtg cgcggcattg atccgcagct cgagacccaa gtgtcgtcga
     1201 tcgcggacgc catgcgcgag ggaaagctca gcgatctcga gccggggaca aatcgcatca
     1261 tcctcggccg catgctggcc taccagctgc aggtgggcgt gggcgatgcg gtaacgatca
     1321 tgatccccgg caacaccgcc tcggcgggcg gtgcaaacga cggcatcgtg ccgcgtctgc
     1381 gggattttca agtggcgggt attttcgagg tcgggctgca ggagcacgac agcgtattgg
     1441 cgctcgtcaa cctgcaggat tccgaggcgc tgcgcggcat cgacggcgcc acgggaatcc
     1501 gcctcaagtt caccgatgtg ctgcaggcac cggagcttgc gcgaattgcc gcctcgcgcc
     1561 tggcgccggg cttgcaggtg cgcgactgga cgcaggacaa cgaagcgtac tttcgcgcca
     1621 tccgcatcga aaagaccatg atgggattga ttctgatgct catcgtcgcc gtggccgtat
     1681 tcaacatcgt ggcgaccctg atcatggtgg tgaacgacaa gcgcaccgac atcgccatcc
     1741 tgcgcaccct gggtttgagt ccacgcggcg tgctggcggt gttcatgacg cagggcctgc
     1801 tcatcggttg gatcggcgcc gccgcgggcg tggcgctggg cctcgccctc gcgctc
//
LOCUS       DASWZT010000207        11059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_54810, whole genome shotgun sequence.
ACCESSION   DASWZT010000207 DASWZT010000000
VERSION     DASWZT010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11059
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54810"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VGE92_05620"
     CDS             <1..364
                     /locus_tag="VGE92_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313329.1"
                     /translation="SVGGAAAIDTETAMTNLLREWKNDQPALLARFDTNHDGTLSHAE
                     WERARAAARQQVMTQAAEMPRPAVNVLTEPGDGRAFLLAASDGESLARRFRVRAFAGV
                     GCFVAASAALTWMLTRVW"
     gene            complement(403..3366)
                     /locus_tag="VGE92_05625"
     CDS             complement(403..3366)
                     /locus_tag="VGE92_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1313330.1"
                     /translation="MGDQSRRRSTNIATVVSGILRALSICVPAALCDPPCALAQAVES
                     TLAAAIPAQPLPQALGALSRQTGLQFVYVSADVREQKSHAVSAGLDAREALTRMLEGT
                     GLKFEYLTPRSIRVLAAEVAAPREPTTKIAARDELQAVIVTANRRDENLQNVPTTIQV
                     LTGETLAKLNATTFDDFVSYLPGVTAHGLGPGQNNIYVRGLATAAQGMQGSGVAGSFP
                     NVAVYLDEQSAQLPDRNLDIYAADLERVEILEGPQGTLFGAGAEAGVVRYITNKPKLN
                     VTEVTVNAGYATTAHGDQSSNVDATLNIPLIADTLAVRGVIYDEQRGGYIDNTPATFA
                     RANTDLGVQYFFGGKVPANNVVINNAGLVANDINPVTYKGVRVEALYQFNEDWNALLA
                     QSYQNMQADGVFAEMAANSLGEPQPDLTVQLFNPSYDKDRFENTALTLDGRVGALKLV
                     YAGAYLVRNVEQVQDYTNYARAELADYYQCVNPGKTAATAQCFTPSSTWHDSERNTHQ
                     SHELRLSTPDDWRIRGIGGLFYENYQIQEQTDWFYLTALPYFHPIGPPTGYFSLNGSM
                     YLPNGTLVPGNTPGRVLVPAPVTSINPNARPLGDSFFDDITRGYRQKAAFASLDVEIL
                     PKTLTLTAGTRYSRTETSEVGAAVGSIGCQLMFEPMAPNPCVDHSNFININAEGLDRT
                     FSGFRSRANLSWKVTEDALLYYTWSQGFRAGGFNRAPFSAEANSPLAPGLASYQAQAD
                     KHGGWVSPLAFAPDSLTNNEFGWRTMWMDRRIQWNGAVYQEDWNNAQIDGGAAGVIDY
                     GVILNGGNYRVRGVETSGVARITTGLTLEAGAAWNHSELVKEATLRWQDGTPIDFRAL
                     KLSNPGGALGSPLAGAPPFQGNIRARYEFGFNGYAPFAQIDAVHQSHSLATTDQLALD
                     LQGNSIAYDLPAFTTYDAALGVGKDGWLVQGYAENLTDTRAQLYANYWQWYKAVTVNR
                     PRTIGLRFSRTFGGK"
     gene            complement(3449..4492)
                     /locus_tag="VGE92_05630"
     CDS             complement(3449..4492)
                     /locus_tag="VGE92_05630"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY1313331.1"
                     /translation="MNSNEYKVRAAIAEQAGEWLVENDAGPLHACDSAALAAWLKSSP
                     VHIEEFLGVSAIARDLKAARTDPEYSLEAIVARARGEDDRLIDAVRGTPARRWLTAGV
                     AMAGCAVLSLGLLLTWNLRPVEHPPMPAGVTALHFETRHGEQISRRLADGSVLHLNTD
                     SAISIRYGKNERRVTLITGQAEFEVAHELDRAFRVIAGSAEVVDLGTKFDVRLEHDST
                     VVTVVEGQVEIGISNSNPSRAPRFVLSADQQISVTNGVLPAAPSGVDGQSATSWLRRQ
                     IVFDHEPLERVAAEFNRYSQKPIEITTPALRNLQITGVFATDDTDAFVAFLRSLKGVR
                     VEVTATRIRVSGD"
     gene            complement(4495..5010)
                     /locus_tag="VGE92_05635"
     CDS             complement(4495..5010)
                     /locus_tag="VGE92_05635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY1313332.1"
                     /translation="MAEPKPSLVERLFADHRGALQTFFLRRIRAKADAADLAQEVYVR
                     MLRLNDREAIRNPVHYLYTVANNLVKEHAVLDRRQASGIDIDEAPAHERLETLPEFDG
                     DLDATQRVARLGVVLQQLRPKCRAAVELRFTQGLSYRAIAMHLGVSPQMAKKYVAQAL
                     SHCRRRMARLS"
     gene            5178..5666
                     /locus_tag="VGE92_05640"
     CDS             5178..5666
                     /locus_tag="VGE92_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313333.1"
                     /translation="MKIWICNATLVAAMALTGAAQAESWGEVCSLETLHGAYSFSISG
                     QILEGPGTGPVNGVALTTFDSRGGLRQTDFVVKNGAPGGPKDAFRTGETGTYTVNADC
                     TGSATINFPDATPAQQLVLMFVVADHGRQIRTVVSALYVGPGIGTMTPTPAQISSEAT
                     KL"
     gene            complement(5759..6514)
                     /locus_tag="VGE92_05645"
     CDS             complement(5759..6514)
                     /locus_tag="VGE92_05645"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY1313334.1"
                     /translation="MTIQSNLSGKTALVTGASRGIGRATAVALAKAGARVIVHYSKGE
                     GEATAVVADIRGTGGTADKLNADLRTADGPHVLAERVRGIISGRLDILVANAGIAKAA
                     SIAETTVEDFDALFAVNVRAPFFLVQQLLPVLGEGSSVIFLSSLAAHAAVGELSAYSA
                     TKGAIDTLVKHFASALGERGIRVNAVAPGVVPTEMSSFAKTQAGRDFTLSVQALKRLA
                     TPEDIAAAIAFLASDQAQWITGDTLRVDGGSKL"
     gene            complement(6544..7164)
                     /locus_tag="VGE92_05650"
     CDS             complement(6544..7164)
                     /locus_tag="VGE92_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY1313335.1"
                     /translation="MNRPKTTQVAVGEEIISSAPKSRVRDRIMKTATDLFYRYGIHAV
                     GVDAIASGAGTNKMSFYRNFASKDELVAEYLRGEEREGLLWWDETIAAHAGNPRLQVE
                     SLFDVLVQNNCEEDSRGCALANAAVEITEPDHPARPVIEKFKADMRRRFRDLAREMGA
                     REPDALGDSLMLLWEGAYLTRVTMGVNGPVQGAAKAARALIGAYTD"
     gene            7292..8581
                     /locus_tag="VGE92_05655"
     CDS             7292..8581
                     /locus_tag="VGE92_05655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY1313336.1"
                     /translation="MPLNRIFHNRSLPVLLGVAALAVFALYSSAGHSRAKPQAALPPA
                     LPEVQVAEVIHRPLREWQEFSGRLQAVNTVEVRPRVSGYVDRVAFTDGARVKKGQLLF
                     QIDPRPFQAEVERLVAERTRSVSDLELAKANRARAERLISAHAISREEYERQVAAETS
                     AQGALGSVDASLQEARLNREFTEVRAPIDGHVSRAIITAGNLVTSASQMTTLVSDDPV
                     YVYFDADEQTYLRYAKAKHEHAPASGGASDVYIGLVDEDGYPHPAQVDFIDNQVDPAT
                     GTIRARAALANPDGRYTPGLFARVRLIGGEDQDSVLIEDRAVGTDLSKKFVLTLTKDN
                     RIEYRLVQLGPQINGLRVVKEGLAPNELIVVNGLQHVRPGQSVAATRVAMSDSAGGLT
                     QVAAQPSVPPASAATAASPVDAVARLAQASVALHVVR"
     gene            8586..>11059
                     /locus_tag="VGE92_05660"
     CDS             8586..>11059
                     /locus_tag="VGE92_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1313337.1"
                     /translation="MKLSQFFIGRPVFAGVLSALIFLAGLIALPKLPISEYPEVVPPT
                     VIVKANYPGANPKVIAETVASPLEQAINGVEGMLYQSSQATADGVMTLTITFALSTDV
                     DKAQVQVQNRVAQALPKLPDVVQRLGVVTNKASPDLTMVVHLVSPDNRYDTLYLSNYA
                     LLRVKDELARLDGVGDVQVFGAGQFSMRIWLDPDRVASRGLTADDVVKAIREQNIQVA
                     AGQLGAPPMPSATEFQVAINAKGRLTTEDDFRGIIVKTGAAGQVTRLKDVARVELGAD
                     SYALRALLDNKAAAAIPIFQRPGSNSIAISELVRAKMQELKRDFPQGVDYQIVYDPTV
                     FVRGSIHAVVHTLLEAILLVVLVVIVFLQTWRASVIPLIAVPVSLVGTFAVMHLFGFS
                     LNALSLFGLVLAIGIVVDDAIVVVENVERNITNGLKPIEATRQAMREVTGPIVATALV
                     LCAVFVPTAFISGLTGQFYKQFALTIAISTVISAFNSLTLSPALSALLLKEHGAPPDR
                     LTRVLNRLLGGFFRPFNRFFTRFSARYVNGVGHVVRAGGVALFIYGGLIALTYLGFAR
                     TPTGFIPPQDKQYLVSFAQLPDAATLDRTEAVIRRMDEIALKQPGVDHAVAFPGLSIN
                     GFTNSPNSGIVFVTLKPFEERKSKELSAGAIAAALNQQYGGIEEAFIAVFPPPPVMGL
                     GTIGGFRMQIEDRGGLGFEELYKQTQNLLAKGNQTPALHGLFSGYQVNVPQIDAEVDR
                     EKAKSQGVSVQELFDTMQVYLGSLYVNDFNAFGRTYPVTAQAEPSFRLQAEDVVRLKT
                     RNAAGDMIPLGSFVTVKHSSGPDRVM"
BASE COUNT         2048 a         3484 c         3385 g         2142 t
ORIGIN      
        1 cagcgtcggc ggcgccgctg caatcgacac cgagactgcg atgaccaacc tgctgcgcga
       61 atggaaaaac gaccagcccg cgctgctcgc gcgcttcgac accaaccacg acggcacctt
      121 gagccatgcc gagtgggagc gggccagggc ggccgcccgt cagcaggtca tgactcaggc
      181 ggccgagatg cctcggcccg ccgtgaacgt tctcaccgag cccggcgacg gccgtgcctt
      241 tctgctggcc gcctccgatg gcgaatcgct cgcgcggcga ttcagggtgc gcgccttcgc
      301 cggcgtcggc tgcttcgtcg cggcgagcgc cgccctcacc tggatgttga cccgcgtctg
      361 gtaaaatgtt gtgtgtgtgt gtgaacgacg ttgaagcctc ggctactttc cgccgaacgt
      421 acggctgaac cgcaatccga tggttcgggg acgattcaca gtgacggctt tgtaccactg
      481 ccagtagttt gcatacagtt gcgcccgtgt gtccgtgagg ttctctgcgt agccttgcac
      541 gagccacccg tcctttccaa caccgagcgc cgcatcatac gtagtgaaag cgggcagatc
      601 ataagcaatg gagttgcctt gcaggtcaag cgcgagctga tcggtcgtag cgagggaatg
      661 tgactgatgc accgcgtcga tctgagcgaa tggcgcataa ccgttgaagc cgaattcgta
      721 acgtgcgcgg atgttgccct gaaagggggg cgccccggcg agcgggctgc caagtgctcc
      781 gcccggattc gaaagtttaa gcgcgcggaa gtcaatcgga gttccgtcct gccaccggag
      841 tgtcgcttcc ttgaccagct cgctgtgatt ccaggccgct cccgcctcga gggtgagccc
      901 ggtggtgatg cgggccacgc ccgaggtttc cacgccgcgc acccggtagt taccaccgtt
      961 gaggatcact ccgtaatcga tgacaccggc ggcacctcca tcgatctgtg cgttgttcca
     1021 gtcctcctga taaaccgcgc cattccattg gatacgccgg tccatccaca tcgtcctcca
     1081 gccgaattca ttgttggtga ggctgtcggg cgcaaaggcc aggggcgata cccagccgcc
     1141 gtgcttgtcg gcttgcgctt gataggaagc gaggccgggt gccagcggcg aattggcctc
     1201 tgctgaaaac ggtgcccgat tgaagccgcc tgcgcgaaag ccttgcgacc atgtgtagta
     1261 cagcagcgca tcctcggtca ctttccagct caggttcgcg cggctcctga agccggaaaa
     1321 cgtccggtca aggccctccg cattgatatt gatgaagttg gaatgatcca cacagggatt
     1381 cggtgccatc ggttcaaaca tgagctggca accaatactt cccacggccg ctccgacttc
     1441 cgaggtctcc gtacgggaat agcgcgtgcc ggccgtcagc gtcagcgtct tcggcaggat
     1501 ctcgacatcg agggatgcaa aggcggcctt ctgtctgtaa ccgcgcgtga tgtcgtcaaa
     1561 gaaactatcg cccagcggcc gggcgttcgg gttgatcgac gtcaccggtg ccggtaccag
     1621 cactcggccc ggcgtattgc ccgggaccag cgtaccgttc ggcagataca ttgatccgtt
     1681 caagctgaag tagccggtgg gcggcccgat cggatggaag tacggaagcg ccgtgagata
     1741 gaaccagtcg gtctgctcct gaatctggta attttcgtag aatagaccgc cgataccgcg
     1801 gatccgccaa tcatcggggg tgctcaggcg cagctcatgg ctttggtggg tgttgcgctc
     1861 agaatcgtgc caggtcgaac tcggcgtgaa acattgcgcg gtcgcagcag ttttacccgg
     1921 atttacgcac tggtagtagt ccgctaactc ggcgcgtgcg taattcgtgt agtcctgaac
     1981 ctgttcgacg ttgcgcacca gataggcgcc cgcatagacc agcttcaggg cacccacacg
     2041 gccgtcaagg gtcagggcag tattctcaaa ccgatctttg tcgtatgacg ggttgaacag
     2101 ctgcacggtg agatccggtt gcggctcacc gagcgaattc gccgccatct cggcaaagac
     2161 cccgtccgcc tgcatgttct gataggactg tgcaagtaat gcattccagt cctcgttgaa
     2221 ctggtacaac gcctctacgc gcaccccctt ataagtgaca ggattgatgt cattcgccac
     2281 caggccggcg ttgttgatca cgacgttgtt ggcgggaact ttgccgccaa agaagtactg
     2341 aacgccgaga tcggtgttgg cacgtgcaaa ggttgcgggg gtgttatcga tgtagccgcc
     2401 gcgctgctca tcgtagatca cgccgcgcac ggccagcgtg tcggcaatca acggaatgtt
     2461 gagggtggcg tcgacattgc tgctctgatc cccgtgcgcg gtcgtggcgt agcccgcgtt
     2521 caccgtgact tcggtaacat tgagcttcgg tttgttcgtg atgtagcgca ccacacccgc
     2581 ctcggcgccc gccccgaaca aagtgccctg cggaccttcc aggatctcaa cgcgctcgag
     2641 atccgccgcg tagatgtcaa gattgcggtc gggaagctgc gcagattgct cgtcgaggta
     2701 cacggcgacg ttcgggaagc tgccggcgac acccgacccc tgcattccct gcgcggccgt
     2761 cgcaaggccg cgcacataaa tgttgttctg accggggccc agtccgtgcg cggtaacacc
     2821 ggggaggtag ctcacgaagt catcaaaggt cgtggcgttg agcttcgcga gcgtctcccc
     2881 ggtcaacacc tgaatcgtcg tcggcacgtt ctgcaggttc tcgtcgcggc gattggcggt
     2941 gacgatcacc gcttgcagct catcccgggc ggctatcttc gtcgttggtt cgcgcggcgc
     3001 cgcaacctcc gcggccagga cgcggatgct gcgcggcgtc aggtattcga acttaagtcc
     3061 ggtgccctcg agcattcgtg tgagcgcttc acgagcgtcg agccccgcgg aaaccgcgtg
     3121 cgatttctgc tcacgcacat cggcggagac atagacgaac tgcagaccgg tctggcggct
     3181 caacgcgcca agtgcctgcg ggagaggctg tgcgggaata gcggcggcga gcgttgattc
     3241 aacggcctgg gctaacgcgc agggagggtc acaaagtgca gcgggcacgc aaattgagag
     3301 cgcgcgcagt atcccagaca ccacggtcgc gatgttcgtt gatcggcgcc gcgattggtc
     3361 tcccatacgc gatctatcca ggcatccgtg cgggccccga cctcaagcat aacccggacc
     3421 gagagtcagg cgtagtgcgc ccctacacct agtctcccga cacgcgaatg cgcgtcgcgg
     3481 tcacttcgac gcgcaccccc ttgaggctgc gcaggaaggc aacaaatgcg tcggtatcgt
     3541 cggtggcgaa gactcccgtg atttgtagat tccggagcgc cggcgttgtg atctcgatcg
     3601 gcttctgcga atagcgattg aattcggccg cgacccgctc cagcggttcg tggtcgaata
     3661 caatttgtcg acgcagccaa gacgtggcgc tctgcccgtc gacgccactc ggggcggccg
     3721 gcaacacgcc gttggtcacg ctaatctgct ggtcggcgct cagaacgaag cgcggcgcgc
     3781 ggctcgggtt cgaattcgaa attccgattt ccacctgtcc ttcgaccacg gttaccacgg
     3841 tcgagtcatg ctccagacgc acatcgaatt tggtgccgag gtcgacgacc tcagccgatc
     3901 cggcaatcac tcgaaatgcg cgatcgagct catgggcgac ctcgaattct gcctggccgg
     3961 tgatgagcgt gacgcgccgt tcattcttgc cgtaccggat ggaaatcgcg ctgtccgtat
     4021 tgaggtgaag aactgaacca tccgccaagc gacgagagat ctgctcaccg tgtcgcgtct
     4081 caaagtgcag tgccgtgacg cctgcgggca tcggcgggtg ttcgacgggc ctcaggttcc
     4141 acgtcaaaag caagccaaga ctcaacacgg cgcaccccgc catcgccact ccggcagtga
     4201 gccagcggcg cgcaggtgtg ccgcgaacgg cgtcgatcag gcggtcgtcc tcgccccgtg
     4261 cacgtgcgac aatcgcctcc agagagtact cgggatcggt gcgtgccgcc ttgagatcgc
     4321 gcgcgattgc cgacacgccg aggaactcct cgatgtgaac cggcgagctc ttgagccacg
     4381 ccgccagggc cgcggagtcg cacgcatgga gtggccccgc atcgttctcg acgagccact
     4441 cgccggcctg ttcggcgatc gcggcgcgga ccttgtattc gttggaattc atattcaact
     4501 cagccgcgcc atgcggcgtc gacaatgact cagcgcctgc gccacgtact tcttcgccat
     4561 ctgcggcgac accccgaggt gcatcgcgat cgcccggtag gaaagtccct gcgtgaagcg
     4621 cagctccact gcggcacggc atttcggccg aagctgctgc aatacgaccc cgagccgcgc
     4681 gacccgctgc gttgcatcaa ggtctccatc gaactcaggc agcgtctcga gacgctcgtg
     4741 cgcgggggcc tcgtcgatgt cgatgccgct cgcctgtcga cggtcgagca cggcgtgctc
     4801 cttcacaaga ttgttcgcta ccgtgtagag gtagtggacg ggattgcgaa tggcctcccg
     4861 gtcattgagc cgcagcatcc tgacgtagac ctcctgcgcc agatccgctg catcggcctt
     4921 ggcacggatt cgccgcagga aaaatgtctg cagcgcgcct cggtggtcag cgaacagccg
     4981 ctcgaccagc gatggtttcg gctcagccac agccaccgtc catgcaattt cctctcatct
     5041 tgtatatgag tcccaacggc ccgaaatggc aaccacaata agttgcgtcg aatttagagg
     5101 tgtccaattc gcgccccgcg gactccttgt caatgtagtg aaattgaaga cgtttaaatc
     5161 acaagaggaa cacgatcatg aaaatctgga tttgcaatgc aactttggtc gcggcgatgg
     5221 cattgacggg agcggcccag gcagaaagct ggggcgaggt gtgctcgctc gaaaccctgc
     5281 acggtgcgta cagttttagc atcagcggtc aaatcctcga ggggcccggt accggacccg
     5341 ttaacggagt tgccttgacc acgtttgatt cgcgcggcgg actcagacag acggacttcg
     5401 ttgtcaaaaa cggtgccccg ggggggccca aagatgcttt cagaaccggc gaaacaggca
     5461 catacaccgt gaacgccgat tgcaccgggt cggcgacgat caattttcct gacgccacgc
     5521 ccgcgcagca gctggtgttg atgttcgtag tcgcagatca cgggcggcaa attcgcacgg
     5581 tggtctcggc gctctatgtc ggtccgggga tcggcacaat gacaccgacg ccggctcaaa
     5641 ttagcagcga ggccacgaag ctctgagcgg cgcgctcgca gccgcccgtg cttccgagga
     5701 cggcggctgc gagaagcggt gaaacgcgct gcaaccagca acgcgcaaac gccggtttct
     5761 agagcttcga accgccgtcg acgcgcagtg tgtcgccggt aatccactgg gcttgatcgg
     5821 atgcgaggaa ggcaatcgca gcggcgatat cctcgggtgt ggccaggcgc ttgagtgcct
     5881 gcacacttaa agtgaaatcc cgtcccgcct gcgtcttggc aaaactcgac atctcggttg
     5941 gcacgacgcc cggggccacc gcgttgacgc ggatgccccg ttcgcccaga gctgacgcga
     6001 aatgcttgac cagcgtgtcg atcgctccct tggttgcgga gtacgccgac aactcaccga
     6061 cggcggcatg tgctgcgagt gaggacagga atatcacgct gctgccctcg cccaggactg
     6121 gcagcagctg ctgcaccaga aagaacggcg cgcggacatt gacggcgaac aacgcatcga
     6181 aatcctcgac ggtagtctcg gcgatcgatg cggctttggc gattcccgcg ttggcgacca
     6241 aaatgtcgag gcgcccgcta ataatgccgc gcacacgttc ggcaaggacg tgaggtccat
     6301 ccgccgtccg caagtcagcg ttgagcttgt ccgcggtgcc gcctgtgccg cggatgtcgg
     6361 cgacgacggc cgtcgcttcc ccctcgccct tgctgtagtg aacgataacg cgagcgccgg
     6421 cctttgcgag cgccacggcg gtcgcgcggc cgatgccgcg agaggcgccg gtgaccagcg
     6481 cggtctttcc tgagagattg ctttgaatgg tcatgtcgta ggcctgaagg gtggcgattt
     6541 aattcagtcg gtgtacgcgc cgatcagcgc ccgtgccgct ttcgcagcac cttgcaccgg
     6601 gccgttcacc cccatggtga cgcgggtcag ataggcacct tcccataaca gcatcaacga
     6661 gtcgccgagc gcatcgggtt cgcgcgcgcc catctcgcgc gcgagatcgc gaaagcgccg
     6721 gcgcatgtcg gccttgaact tctcgatcac cggacgcgcc ggatgatcgg gctcggtgat
     6781 ttccacggcg gcattggcga gcgcgcaacc ccgcgaatct tcctcgcaat tgttctgcac
     6841 caacacatcg aacagcgact cgacttgcag acgcggattg ccggcgtgcg ccgcgatggt
     6901 ttcatcccac cagagcaggc cctcgcgctc ctcgccgcgc aagtattccg ccaccagctc
     6961 gtcctttgag gcgaagttgc gatagaagct catcttgttg gtaccggccc cgctggcgat
     7021 ggcatcgacg cccacggcgt gaatcccata gcgataaaac agatcggtgg ctgttttcat
     7081 gatgcggtcg cgcacgcggg acttcggcgc cgaagaaata atctcttccc ctacagccac
     7141 ttgcgtcgtc ttaggtcgat tcataaagga ctccacttga catgtgactg accggtcagt
     7201 aacttactac ccaaggtgac tgaccggtaa cacacaccac atagcatcga tgcgtttcag
     7261 tcgtaagtca agttcccata ggagtctgcc cgtgcccctc aatcgcatct ttcataatcg
     7321 ctctctaccc gttctgctcg gtgtcgccgc tctcgccgtt tttgcgctct attcgagcgc
     7381 cgggcattct cgcgccaagc cgcaggcggc cttacccccc gccttgcctg aagtgcaggt
     7441 ggcggaggtg atccaccggc cgctgcgtga atggcaggaa ttcagcggtc gtctgcaggc
     7501 cgtgaacacc gtggaggtgc gtccgcgggt gagcggctat gtcgaccgcg ttgccttcac
     7561 cgatggcgca cgcgtgaaga aaggccagct gttgttccag atcgatccgc ggccattcca
     7621 agcggaagtc gagcggctgg tcgcagagcg tacgcgcagc gtctccgacc tcgaactcgc
     7681 caaggccaac cgtgcacgcg ccgaacgcct gatcagcgca cacgccatct cgcgtgagga
     7741 atacgagcgc caggtcgcgg ctgaaacctc ggcccaaggc gcgttgggtt ccgtcgatgc
     7801 gtcattgcaa gaggcacgtc tgaatcgaga gttcaccgaa gtgcgcgccc cgatcgacgg
     7861 tcacgtctcg cgcgccatca tcactgcggg aaacctcgtc acgagcgcga gccaaatgac
     7921 gacgctcgtt tccgacgacc cggtctatgt ttacttcgac gcggacgagc agacctattt
     7981 gcgctacgcc aaggcgaagc acgagcatgc gcccgcgagt ggcggcgcct ccgacgtcta
     8041 tatcggactc gtcgacgagg acggctatcc gcacccggcc caggtggact tcatcgacaa
     8101 ccaagtcgat ccggccaccg gcacgattcg cgcgcgcgcc gcactggcaa atccagacgg
     8161 gcgctacacg cccggcctgt ttgcgcgagt tcgcctcatc ggcggggaag atcaggacag
     8221 cgtcctcatc gaggatcgtg ccgtgggtac ggatctttcc aagaaattcg tgctgacgct
     8281 gaccaaggac aaccgcatcg agtatcgcct ggtgcaactt gggccgcaga tcaacggcct
     8341 gcgcgtcgtg aaagaaggcc ttgcgccgaa cgagctgatc gtcgtcaacg gtttgcagca
     8401 tgtgcggccc gggcaaagtg tcgccgccac gcgcgttgcc atgagcgatt ccgcgggcgg
     8461 gctgacccaa gtcgccgccc aaccgagcgt gccgccggcg agcgcagcga cggctgccag
     8521 tccggtcgat gcggtcgcgc gtcttgcgca ggcgagcgtc gcccttcacg tagtgcggtg
     8581 aagtcatgaa actctcgcaa tttttcatcg gccgtcccgt tttcgcgggg gtcctctcgg
     8641 ccctcatctt ccttgcgggc ctgatcgcgc tgcctaagct tccgatcagc gagtatccgg
     8701 aggtcgtgcc gccgacggtg atcgtcaagg cgaattaccc gggcgcaaac cccaaggtca
     8761 ttgccgagac cgtcgcgagt cctctcgagc aggcgatcaa tggggtggag ggcatgctct
     8821 accagtcatc gcaggcaact gccgatggcg tgatgactct caccatcaca ttcgccttga
     8881 gtaccgatgt cgacaaggcg caggtgcagg tgcagaaccg cgtggcccag gccctgccga
     8941 aactccccga cgtcgtgcag cgtctcggtg tggtcaccaa caaggcctcg cccgacctga
     9001 ccatggtcgt gcacctggtt tctcccgata atcgctacga cacactgtac ttatcgaact
     9061 atgcgctgct gcgggtcaag gacgaactcg cccgcctgga tggtgtcggc gatgtgcagg
     9121 tattcggcgc aggccagttc tcgatgcgca tctggctcga tccggaccgg gtcgcaagtc
     9181 gcggcctcac ggcggatgat gtcgtcaagg cgatccgcga gcagaacata caagtcgccg
     9241 cgggtcagct cggcgcgccg ccgatgccga gtgcgacgga gtttcaggtg gcaatcaatg
     9301 ccaaggggcg cctgaccacc gaggatgatt ttcgcggcat catcgtcaag accggcgccg
     9361 ccggtcaagt cacgcgtttg aaggacgtcg ctcgcgtcga gctcggcgcc gactcctatg
     9421 ctctgcgcgc gctgctcgat aacaaggcgg cggcggcgat accgattttt caacggcccg
     9481 gcagcaattc gatcgcgatc tcggaactcg tgcgcgcgaa aatgcaggag ctgaagcgcg
     9541 atttcccgca aggcgtcgac taccaaattg tctatgaccc caccgttttc gtgcgcggtt
     9601 ccatccacgc ggtggtgcac acgctgctcg aggccattct gctggtggtg ttggtggtca
     9661 tcgtcttcct tcagacctgg cgtgcttccg tcattccctt gatcgccgtt ccggtatcgc
     9721 tggtcggaac ctttgcggtc atgcacctat ttggctttag cttaaatgcg ctgtccctct
     9781 ttggcctggt gctggccatc ggcatcgtcg tcgacgatgc gatcgtcgtg gtcgagaacg
     9841 tcgagaggaa catcaccaat gggttgaagc cgatcgaggc gacccgccag gcgatgcgcg
     9901 aggtgaccgg tccgatcgtc gcgacagcgc tggtgctgtg tgcggtgttc gtgcccacgg
     9961 cattcatcag cgggctcacc ggccagtttt acaagcagtt cgccctgacc atcgccatca
    10021 gcacggtgat ctcggccttc aactcgctca cgctctcgcc ggcattgtct gccctgttgc
    10081 tcaaggaaca cggcgcaccg cccgatcggc tgacccgcgt cctcaatcga ctactcggcg
    10141 ggttcttccg cccgttcaat cggttcttca cgcgcttctc cgcgcgctac gtcaacggcg
    10201 tgggccatgt ggtgcgcgcc ggcggtgtcg cgctctttat atatggtgga ttgatcgcgc
    10261 tgacttactt gggcttcgcg cgcacgccca ccggtttcat tcctccccag gacaagcagt
    10321 acctggtgag cttcgcgcag ctccccgatg cggcgaccct cgatcgcacc gaggcggtga
    10381 tacgccggat ggacgagatt gccttgaagc agccgggcgt cgaccacgcg gtggccttcc
    10441 ccggcttgag catcaacggc ttcaccaata gtccgaactc cggcatcgtg ttcgtgacct
    10501 tgaaaccctt cgaggagcgc aaatccaaag agctctcggc cggcgccatc gccgctgcgc
    10561 tcaatcaaca gtacggcggt atcgaggagg cctttattgc ggtgttcccg ccgccgcccg
    10621 tcatgggact tggcaccatc ggcggctttc gcatgcaaat cgaggaccgg ggaggcctcg
    10681 ggttcgagga actctacaag cagacgcaga acttactggc gaagggcaat caaaccccgg
    10741 cgctgcacgg gctcttttcg ggctaccagg tgaatgtccc gcagatcgac gccgaggtgg
    10801 atcgtgagaa ggcgaaatcg caaggcgtgt cggtgcagga actcttcgac accatgcagg
    10861 tgtacttggg ctcactctac gtcaatgact tcaacgcctt tggccgcact tatccggtaa
    10921 cggcccaggc cgagccgagt ttccggctcc aggccgaaga tgtcgtgagg ctcaagactc
    10981 gcaatgccgc gggcgacatg atcccgttgg gatccttcgt gaccgtcaag cactcctcgg
    11041 gtcccgatcg cgtcatgca
//
LOCUS       DASWZT010000208         6137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_54814, whole genome shotgun sequence.
ACCESSION   DASWZT010000208 DASWZT010000000
VERSION     DASWZT010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6137
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54814"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VGE92_05665"
     CDS             complement(<1..695)
                     /locus_tag="VGE92_05665"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEY1313338.1"
                     /translation="MLCAAFFAGFIDAIVGGGGLVQVPALLAAYPAEAPALLLGTNKL
                     GSICGTASAVLRYMRVVRIPWRVLLPACMAAFLAALAGAGLVSTVSPLLFRPLVPIML
                     SVVLIYVLWQKDLGARHAPVVFSRRRSCVALAAVSGIGLYDGFFGPGTGSFLMLLFIR
                     LYGFDFLHASVGARTINVATNAGALVFFGIHRDIHWALGIALGACNAVGSVLGAHTAI
                     KRGSKFVRIVFIG"
     gene            complement(722..1666)
                     /gene="nudC"
                     /locus_tag="VGE92_05670"
     CDS             complement(722..1666)
                     /gene="nudC"
                     /locus_tag="VGE92_05670"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEY1313339.1"
                     /translation="MQQRNVLAGPYLDRVAHLRQDPAWFAAAFADERSRAIPVWNSRS
                     LIVEGNTPRAAYLPLHQVPPERRSGLDLVLLGRSGDTHYFTYDIESAEPPPMPPGTRF
                     EDLRLIAAALPMDEAGLLSYARAIISWRRRNRFCGACGASTVPAKSGHVLVCTNPSCR
                     HEQFPRLDPAIIVLVGEGDRALLGRQASWPVGRYSTIAGFVEPGESLEDAVAREVFEE
                     TGIEVDRIEYHSSQPWPFPASLMLGFTAHAITTEVHLNDQELEDARWFTRAELAANGT
                     LLPPRQSISFRLIEDWFDAGGGTPLRDIHRSTTWAQSR"
     gene            complement(1874..2923)
                     /locus_tag="VGE92_05675"
     CDS             complement(1874..2923)
                     /locus_tag="VGE92_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313340.1"
                     /translation="MNAGSKWAPQVAAASALSAAFRVITHRGVQVAFTLWVIGYLLVF
                     WIAGGSLPFDRPAVATRSFALQLAAPSITLIEILILTGLVFWLSRGRIVPDIARRAPH
                     RPLALRETLLLIGYAALGQVGGWILGPALGYRAFSFHIAGTVFGCSVAPQRGEMCTWA
                     LYNFVIFALLPYLYFRRRYSNTQLNLRSTDRRNDLLLIVAVIVVESAFELAIFDNTLL
                     SLSARQILIGAPLTLLIFFIGTVLPTMILIYAILLPRYLALSGSRITTVLLGGLTYAA
                     MHIVEGWSTFNSPTNTTLSLIFVLLQYFGPGMFKSVLTLRTGNAWVHALGYHMIAPHM
                     IVDTPLVVKTFGIVA"
     gene            3173..5749
                     /locus_tag="VGE92_05680"
     CDS             3173..5749
                     /locus_tag="VGE92_05680"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEY1313341.1"
                     /translation="MVTASQTTMPPTKAATREVLPDSVIPVHYELALSPDAEALTFHA
                     KVTITVEVRIKTAAITLNEVGLSFDHIVVDGDRQGIMRSDEKLGRATITVGVPLAPGI
                     HVLLIDYHGKIGRSTLGFFAMDYTGPDGPRRTLATNFEPAAARQLLPCWDEPARKATF
                     TVSVVAPKDRMVISNMPVAEVTSLSASLRRVRFAQTPKMSTYLLFVGVGDFERIHSIV
                     DGVDVGVIVKRGDTGKAAYALEQAGALLHYYNGYFGTPFPLPKLDLIAAPGQISGGSM
                     ENWGAIFYSQNHLLFDPASSTEEDRQLVFLVVSHEMAHQWFGDLVTMAWWSDLWLNEG
                     FARWMQTFAADDLHPEWQTGLQAMSIFESGKSADSVPSTHPVVQRVDTADQAAESFDE
                     ITYDKGAAIITMMNAYVGRDKFREGVRRYMRAHAFGNTVDTDLWRLVGQTVGQEIVAI
                     ERDFTRQEGLPLVRATGSAQGLRLVQARFADDPATIAELPPQHWRLPLAIAAVGADAH
                     YHFLQGTATVDQTPPLLVNAGQAGYARVLYDDELLSGLTAQMSSLSSADQMGIINDAR
                     ALGIAGYTSAANLFNVAAALPREASPIVWQRLLEIVREIDRHYADTPARTAFRRFALG
                     MLAPLSVQIGPLGTPGGDAKVQVLRSELTETRGQLGDVPVIEDARKRFAGGDGTPVEQ
                     RCVLAIVAEQADTAGFDALLAKANHTTDPLEKLHIFRALAGVKDSDLARRMVDIALGN
                     QVPAGSAPSLIETLARKHADAVWQMLAPRLDDPSLPFEKTLRWTIAGDIASYSADPAR
                     IAELEAYEAASVPSEARKPFLSAVASIRRNQRFTRTLLPQIDQWIAAHAPAAAMNEGR
                     AP"
BASE COUNT         1238 a         1908 c         1917 g         1074 t
ORIGIN      
        1 atcccgatga aaacgatgcg cacaaacttg ctgccgcgct tgatggccgt atgcgcgccc
       61 agcacgctgc ccacggcatt gcatgcgccc agcgcgattc ccagggccca gtgaatatcg
      121 cggtggatgc cgaaaaaaac caatgcgccc gcgttggtgg cgacattgat ggtgcgggcg
      181 ccgaccgagg catgcaagaa atcgaatccg tataagcgga tgaacagcag catcaagaag
      241 ctgccggtcc cgggcccgaa gaaaccgtcg tacaagccga tgccgctgac tgccgccaac
      301 gccacgcagc tgcggcggcg cgagaagacg acgggtgcat ggcgagcgcc caaatccttc
      361 tgccagagca cgtagatgag caccacgctc aacatgatgg gcacgagcgg acgaaacagc
      421 agcggcgaca ccgtgctcac caaacccgca ccggcaagcg cggcgagaaa cgccgccata
      481 catgccggca acaacacgcg ccatgggatg cgcacgacgc gcatgtaacg cagcaccgca
      541 cttgccgtgc cgcagatgct gccgagcttg ttcgtcccga gcagcagcgc aggcgcctcg
      601 gcaggatagg ccgcgagcag tgcgggcact tgaaccaagc ccccaccgcc gacgattgcg
      661 tcgataaatc cagcgaagaa ggcggcgcac agcaccaccc caaggctcgc gtagctgccg
      721 cctaacgcga ttgcgcccag gtcgtactcc gatgaatgtc gcgcaacggc gttccgccgc
      781 ctgcatcgaa ccaatcctcg atcagccgga acgatatgga ttgccgcgga ggcagcaacg
      841 tgccgttcgc ggcaagttcg gcgcgggtga accagcgggc gtcttccagt tcctgatcat
      901 ttaaatgcac ttccgtggtg atggcgtgcg ccgtaaagcc gagcatcagc gaggccggga
      961 agggccacgg ttgcgatgag tggtattcaa tccggtcgac ttcgataccg gtttcttcga
     1021 acacctcgcg tgccacggca tcctcgagcg attctcccgg ttcgacgaaa ccggcaatgg
     1081 tggagtaacg cccgaccggc cagctggcct gccgtccgag cagggctcga tcgccttcgc
     1141 cgaccagcac gatgatggcg gggtcgagcc tcgggaattg ttcatggcgg cagctgggat
     1201 tggtgcatac gagcacgtgt ccgctcttcg cgggtacggt gctcgcaccg cacgcgccgc
     1261 agaagcgatt gcgccgccgc caggagatga tggcccgcgc ataactcaac agtcccgctt
     1321 catccatcgg cagcgcggcg gcgatcaggc gcaagtcctc gaagcgcgtt ccgggcggca
     1381 tcggcggtgg ctcagccgat tcgatgtcgt atgtgaagta gtgagtgtcg ccggatcggc
     1441 cgagcaaaac caaatccaat ccgctgcgcc gttccggcgg cacttgatgc agcggcaaat
     1501 aggccgcgcg cggcgtgttg ccttcgacga tgaggctgcg tgaattccac accgggattg
     1561 cgcgactgcg ctcatcggcg aaagccgcgg caaaccacgc agggtcctgg cgcaaatgcg
     1621 cgacgcggtc caggtacgga cccgctaaga cattccgttg ctgcatccgt caattctaac
     1681 agggcgcggc ctgatttcag gcccgggcgg cgggaccggc tgccgctgag cggtcattcc
     1741 acgacaggcc tacactgtcg gtcggttgaa gtttgcgctc ggctggatgt tagtgccggg
     1801 tactcacggc gctacccacg accgccgcct atgcgtggtt ggcgtggaat gcattgagcg
     1861 gatgatgcgc gcgtcatgca acgatgccga atgttttgac cacgagtgga gtgtcgacaa
     1921 tcatgtgcgg tgcaatcatg tggtagccga gcgcatgcac ccaggcattg ccggttcgca
     1981 gtgtcaacac gctcttgaac atccccggtc cgaagtattg aagcagcacg aagatgagcg
     2041 agagcgtcgt attggtcggg gaattgaacg tggaccaacc ctcgacaata tgcatcgcgg
     2101 cataggtgag tccacccagc agcacggtcg tgatccgcga gccagataat gcaagatagc
     2161 ggggcagcaa gatcgcatag atcaagatca ttgtcgggag taccgtgccg ataaagaata
     2221 tcagcagtgt cagaggtgct ccaatcagta tttgacgcgc actgagactt aaaagcgtgt
     2281 tgtcaaagat ggcaagctcg aatgcggact cgaccacgat gacagctacg atcaagagca
     2341 aatcgtttcg ccggtcggtc gagcgcaggt tgagttgcgt gttgctatag cggcgccgga
     2401 aatagaggta cggcagcagc gcgaatatca caaaattgta cagcgcccag gtacacattt
     2461 cgccacgctg gggtgcgaca ctgcagccaa agactgtgcc ggcgatgtga aagctaaagg
     2521 cccggtaacc cagcgccgga cccaagatcc aaccgcccac ctgaccgagt gccgcatagc
     2581 cgatcaagag cagtgtctcg cgcaatgcca atgggcgatg aggtgcgcgg cgcgcgatgt
     2641 ccgggacgat acgcccgcgg ctgagccaaa aaaccaaccc cgttagaatg aggatctcga
     2701 tcagcgtgat gctcggggcg gccaactgca gcgcgaaaga gcgtgtggcg acggccggcc
     2761 gatcaaaagg aagtgaacca ccggcaatcc aaaacaccaa tagatatcca atcacccaaa
     2821 gcgtgaaggc aacctggacg ccacgatgcg tgatgactcg aaacgccgca ctcaaagccg
     2881 acgcggcggc aacctgcggt gcccatttac tcccggcatt cattgagcag tgcttccagt
     2941 acgagtgaaa agcggtgtct aagatgaagt cagggggaga ttcgccgaca gttcattcag
     3001 tctcgatagt accccgccag aaattgcccg gacgaacaat tttcgtcgcc cgcaaacctg
     3061 gttgcagcgg ctttcgctgg gtgagagcat gcgccgatag tctaaaaacc acgggggatt
     3121 catgacgttc attcggccgt tggttgggct tgtgcttatc tgcgccgcgg gaatggtcac
     3181 ggcgtcccag acgacgatgc cgccaaccaa ggccgccacg cgggaagtcc ttcccgacag
     3241 cgtgatcccc gtgcactacg agctcgcact ttcgcccgat gccgaggcgc tcacctttca
     3301 tgcgaaggtg acgatcaccg tcgaggtgcg catcaagacc gccgccatca ccctgaacga
     3361 ggtcggcctc agcttcgatc acatagtggt ggatggcgat aggcaaggca tcatgcgcag
     3421 cgatgagaag ctcggccgcg ccaccatcac ggtgggcgtt ccgctcgcgc ccgggataca
     3481 tgtcctgctg atagactatc acggcaagat cggccgctcg accttgggct tcttcgccat
     3541 ggattacacc ggacccgacg gaccgcggcg cacgctcgcg accaattttg agccggccgc
     3601 ggcgcgccag ctgctgccct gttgggacga acctgctcgc aaggccacgt tcaccgtttc
     3661 ggtggtcgcg cccaaggacc gcatggtgat ctctaacatg ccggtggcgg aggtgacgtc
     3721 gttgtcggcg agtctgcggc gcgtacgctt cgcgcaaact ccgaagatgt cgacatacct
     3781 gctattcgtc ggcgtgggcg acttcgaacg cattcactcg atagtggacg gcgtggacgt
     3841 cggggtcatc gtgaagcgcg gcgataccgg caaggccgcc tatgccctcg agcaggcagg
     3901 cgcactcctt cattactaca atggttactt cggcacgccg ttcccgttgc cgaagctcga
     3961 cctgatcgcc gcgcctggcc aaataagcgg cggttcgatg gagaactggg gcgcgatttt
     4021 ctattcacag aatcacctcc tgttcgatcc tgccagctcg accgaagagg accggcagct
     4081 ggtattcctg gtagtcagcc atgaaatggc ccatcagtgg ttcggcgatc tggtgaccat
     4141 ggcctggtgg agcgatctgt ggctgaacga gggtttcgcg cgttggatgc aaaccttcgc
     4201 ggccgacgac ctgcatccgg aatggcagac tggcctgcag gccatgtcga ttttcgagtc
     4261 cggcaagagc gctgattcag tgccttcgac tcatcccgtg gtgcagcgag tcgacacggc
     4321 agaccaagcg gccgagtcct tcgatgagat cacctacgac aagggtgcgg ccatcatcac
     4381 tatgatgaac gcctatgtcg gacgcgacaa atttcgcgag ggagtgcgcc gctacatgcg
     4441 cgcgcatgct ttcggcaaca ccgtggacac ggatctgtgg cgtctggttg ggcaaaccgt
     4501 gggtcaggag atcgtcgcca tagagcggga cttcacgcgc caggaggggc tgccgctggt
     4561 gcgcgcgacc ggctcagcgc aaggcttgcg cctcgtgcag gcgcgcttcg ccgacgatcc
     4621 cgccaccatc gccgagctgc cgccgcaaca ctggcggctg cccctcgcca tcgccgcggt
     4681 gggagccgac gcgcattatc atttcttgca aggcaccgcc acggtcgatc agacgccgcc
     4741 gctcctggtc aacgcgggac aggcgggtta cgcgcgggtg ctctacgacg atgagctgct
     4801 ttctgggctg acggcgcaga tgagctcgct gagttctgcc gatcagatgg gaatcatcaa
     4861 cgatgcgcgc gctctcggga tcgccggcta cacctccgcg gccaatctgt tcaatgtcgc
     4921 ggcggccctg ccgcgcgagg ccagtcccat cgtctggcag cggctgctcg agatcgtgcg
     4981 cgagatcgac cgccattatg ccgacacgcc ggcgcggacc gcattccgcc gcttcgcgct
     5041 cggcatgctt gcgccgctgt cggtgcagat cggaccgctc ggcacgccgg gtggcgatgc
     5101 caaagttcag gttctgcgca gcgagctcac cgagactcgg ggccagctcg gcgatgtacc
     5161 ggtgatcgag gacgcacgca aacgctttgc tggcggcgat ggcacgccag tcgagcagcg
     5221 ctgcgtcctc gctattgtgg ccgagcaagc cgacacggcc ggcttcgacg cactgctcgc
     5281 caaggcgaac cacaccaccg acccgctgga gaaactgcat atctttcgcg cgctggccgg
     5341 agtcaaggac tcggatttgg cgcgccgcat ggtagacatc gcgctcggca accaggtgcc
     5401 cgcgggcagc gcaccctcgc tcatcgagac cctcgcccgc aagcacgccg acgcagtctg
     5461 gcagatgctc gcgccgcggc tcgacgaccc ctcgctgcca ttcgagaaga cgttgcgctg
     5521 gacgatagca ggggacatcg cgagctattc cgccgatcct gcgcgcattg ccgagcttga
     5581 ggcgtacgag gccgccagcg tgccgtccga ggcgcgcaag cccttcctgt ccgcagtggc
     5641 atcgatccgc aggaatcaac gcttcacgcg caccttgtta ccgcaaatcg accagtggat
     5701 cgcggcgcat gcgccggccg ctgcgatgaa cgaggggcgc gccccgtaac ctcatcttgg
     5761 gtcccgccgg cccgcgcgcg tggtcgaagc gcggggcgca ccgcgtcagg tgaacgtact
     5821 aaaccgattt gcgatggtgc gttgcgtcag agatttttca gcgggttgtt ccgagtgacg
     5881 tgttgcgcgt cctgagtgac gatcgttctc ccgctagctg caaatacctt ctcaagaatt
     5941 ccgtcgccgc gccgacagct aattcacgcc atggtgggca atgagcgccg cggcttagag
     6001 agtgagctcg tcgtcttacc aaggtgcgca gtgacaaaaa catggactag ccgagcgcgt
     6061 cgcaccatca gtgcggacgc attgcgccct aattggccgc tgttgttcct gggattcttg
     6121 tgcgctctgt gcaacac
//
LOCUS       DASWZT010000209         5027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_45114, whole genome shotgun sequence.
ACCESSION   DASWZT010000209 DASWZT010000000
VERSION     DASWZT010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45114"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VGE92_05685"
     CDS             <1..457
                     /locus_tag="VGE92_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313342.1"
                     /translation="MEPLTPLARDAEPQVRAQPAAKAEAPPPKAELKTETVPVPPIRV
                     AAAPANEPAAEPAPPAKVEFGIDLGGAATLDALRLHWAAMKANYGPLLIGLHPLASQH
                     PKHPSGVDYRLVAGPLPNTAEATRLCARFPVTRTGCRPAKFDGTQLAEH"
     gene            493..867
                     /locus_tag="VGE92_05690"
     CDS             493..867
                     /locus_tag="VGE92_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313343.1"
                     /translation="MKLFLPSARATNYLLIVGFCSLGYALYIRYLAIEQATVGLACAA
                     GLDTWLCFSRRIAIALFEYTVFGWAALAVALVNLLRPTLVVFAVALSIACFGVVLYNS
                     GLSALALGLLVLSFARRAPEPD"
     gene            complement(833..2428)
                     /locus_tag="VGE92_05695"
     CDS             complement(833..2428)
                     /locus_tag="VGE92_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313344.1"
                     /translation="MRLGLACLAVSALVIAGIWAWLGAPLAMPETPLSPGEKLYCLSY
                     SPFRGSQTPLDPTTRIPAAQMEEDLSRLAKITDCVRTYSTEFGQDEIAGIAARHGLKV
                     MQGLWLSGKADKNREQIESVVALANKYPGTIRSVVVGNEVLLRGEMSSSDLAATIRAV
                     KARVPVPVTYADVWEYWLHNADVAAAVDFITIHILPYWEDFPIPARTAAAHVDSIRRQ
                     VAAAFAGKEVIIGEVGWPSAGRMREGALPSPANQARVIQDVLALAKREKFRANVIEAF
                     DEPWKRALEGTVGGHWGLLDDVTRTPKFVWGEAVSNHPHWIVQMAGGILFAVIVFAAA
                     LVARRGAVPSVIWLAVTANAIAGGALIGWGIASASVESLGVAGWLRSLALVCVALLAP
                     PVLSATLMRGTAVPRLSRLIGPAAGRIREPLARLTGAILIATMLLAILSALGLVFDPR
                     YKDFPFAPLSAAIVPFLTHSLTMPRPNGARGIAELAGASLLALSACYIALNESFANWP
                     SLWLCAAFAALAFSLARVRDAQS"
     gene            2699..3229
                     /locus_tag="VGE92_05700"
     CDS             2699..3229
                     /locus_tag="VGE92_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313345.1"
                     /translation="MAFIKTGGTMPITSLLRAFCTAFCVVAFGLAGSAAYAQSGNGTP
                     PEQQKSAVDAAKEGQKSIDEIAEASRLLTGPAGNPECVWLGRRVVSLLWRDDLDTAFR
                     HLDLYDRFGCPSSHIQATFRCLVRQGHIDPKAQDSLNGRVHLCWLNPALTPAPAAAAA
                     QPPAAATATSGGTTPH"
     gene            3416..>5027
                     /locus_tag="VGE92_05705"
     CDS             3416..>5027
                     /locus_tag="VGE92_05705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY1313346.1"
                     /translation="MRTVAAVIALVACVQAGLWAAGQDHLSAPNIDGQFASVSYAPFA
                     SSVNPDEGGKAQEAQIRADLQKLAPLTRAIRTYSSTGGVELVPGIAAEFGLRATIGAW
                     IDKNQDRNEREIRSVVELAKRHSNVNGIIVGNETIYRGELKVPELIQLIQRVKRQSNV
                     PVTTGEIWNVWIEHPELVSSVDYIAAHILPYWEGFSEGKAVDQAIIIYDKLRQAYPGK
                     RIVIAEFGWPSAGYNMHNANPGRVEQAVVLRDFISRAEALGIDYNIVEAIDQPWKIFE
                     GGVGPYWGLLDASRHQKFEWTGPITDPDHWKLAGIALLVSVLLSLPILAMTAASFWQT
                     VMLAAAANVVGAWFAAVFAYWNGHYFVPGAAFALGLGVVLLIPLVIIALARIDELAMI
                     AFGRKPQRLVGSPSLVPQLPEAATPKVSIHLPAYNEPPEMLKQTLDAMARLDYPNFEC
                     VVVINNTPDPALWRPIEEHCQALGERFKFVNADTLQGFKAGALRLAMVHTAADAEVIG
                     VLDADYVVQPNWLKDLVPLFRDATVGLVQ"
BASE COUNT          933 a         1662 c         1548 g          884 t
ORIGIN      
        1 catggagccc ctgacgccgc ttgcgcgaga tgcggagccg caggtcaggg cgcagcccgc
       61 ggcgaaagcc gaggccccgc cgcccaaagc tgagctaaaa accgaaacag ttccggttcc
      121 gccgattcgc gtcgccgcag cccccgcgaa cgagccggca gccgagcccg cgccgcctgc
      181 gaaggtagaa ttcggcattg atctcggcgg cgcagccacc ctcgacgcct tgcgcttgca
      241 ttgggcggcc atgaaggcca attacggacc gctgttgatc ggtctgcacc cgctcgcctc
      301 gcaacacccg aaacatccct ccggcgtcga ttatcgcctc gtggcgggcc cgctgccgaa
      361 taccgccgag gccacaagac tgtgcgcccg cttcccggtc acgcgcaccg gctgccgtcc
      421 ggcgaagttc gacggcacgc aattggccga gcattgattg tcggcgcgcc tgcgttccag
      481 cataatcccc gcatgaaact gtttttgccg agcgcgcgcg ctaccaatta tttgctgatc
      541 gtgggcttct gctcgctcgg ctacgcgctc tacatccgtt atctcgccat cgagcaggcg
      601 acggtcggtc tggcctgcgc ggccgggctc gacacttggt tgtgcttttc gcggcgaatt
      661 gcaatcgcgc tgttcgagta cacggtattc ggttgggccg ccctcgcagt tgcgctcgtc
      721 aacttgctgc gcccgacgct ggtggtgttc gcggtcgcgc tcagcatcgc ctgcttcggc
      781 gtggtgctct acaatagcgg tctgtcggcg ctggcgctcg gactgctggt tctcagcttt
      841 gcgcgtcgcg cacccgagcc agactgaagg caagcgccgc gaacgctgcg cacagccaga
      901 gcgacggcca gttcgcgaaa ctctcattga gcgcgatgta acaagccgac aatgcgagca
      961 ggcttgcgcc ggccaattcg gcgatgccgc gcgcgccatt tggccgcggc atcgtcaggc
     1021 tgtgggtcaa gaacggcacg atcgccgcgc tcagcggcgc gaacggaaaa tccttgtaac
     1081 gcggatcgaa taccagaccg agcgcggaca aaatcgccag cagcatggtc gcgatcagga
     1141 tcgcgccggt cagccgcgcc agcggctcac gaatgcgccc ggccgcggga ccgatcaggc
     1201 gcgacaaccg cggcaccgcc gtcccgcgca tgagcgtcgc gcttaaaacc ggcggcgcca
     1261 acagcgcgac gcagaccaag gccagcgagc gcaaccagcc ggcaacacca agagattcga
     1321 ccgacgcgct ggcgatgccc caaccgatca acgcgccgcc ggcgatcgcg tttgccgtca
     1381 ccgccaacca aatgacagac ggcaccgcgc cacgtcgcgc caccaacgcc gcggcgaata
     1441 caatcacggc aaataaaatt ccgcccgcca tctgtactat ccaatgcgga tgattggata
     1501 ccgcctcgcc ccacacgaat ttcggcgtgc gggtgacgtc atcgagcagc ccccagtgac
     1561 cgccgacggt gccttcaagc gcgcgcttcc acggctcatc gaatgcctcg atcacgttgg
     1621 cgcgaaactt ttcgcgcttg gcaagcgcca gcacgtcctg gatcacacgc gcctggttgg
     1681 ccggcgacgg cagcgcgccc tcgcgcatgc gcccggcgct cggccagccg acctcgccga
     1741 taatcacttc cttgccggca aaagcggccg ccacctggcg gcgaattgaa tcgacatggg
     1801 cggcggcggt gcgcgccgga attggaaagt cttcccagta aggcagaata tgaatcgtga
     1861 tgaagtcgac cgcggcggcg acgtcggcat tgtgcaacca gtattcccac acatcggcgt
     1921 aggtcaccgg caccggaaca cgtgccttca ccgcgcggat ggtggctgcc aggtcggatg
     1981 acgacatctc gccgcgcaac agcacttcgt tgccgaccac cacagagcgg atagtgccgg
     2041 ggtatttgtt ggcgagcgcg acgacggatt cgatctgctc gcggttcttg tcggccttgc
     2101 ccgacagcca taggccctgc atcaccttga ggccgtgacg cgcggcgatc ccggcgatct
     2161 cgtcctgtcc gaattcggtc gaataggtgc gaacgcaatc ggtgattttg gcgaggcgcg
     2221 acaaatcctc ctccatttgg gcggctggaa tccgggtcgt gggatcgagc ggggtctgac
     2281 tgccgcggaa cggcgagtac gacaggcaat agagcttttc gcccgggctc agaggcgttt
     2341 cgggcatagc cagcggggcc ccgagccagg cccagatgcc ggcgatgacc agcgccgaca
     2401 cagcgaggca ggctagacca aggcgcatcg cgcttaaccc aagttcccgc ggaaaaaccc
     2461 cgggaagggc ttatagcgcg ggcctgacgc tgccaagctc ggctcttaag caaaccgcag
     2521 ggtggcgctg gacaaagtcc ggccgttgag gccaactctt tcggcaggag aattccgcca
     2581 agagtggcgt ttcgggtcga gagttcaggt gtttagggca acagcgccgg tacggccggc
     2641 agcaagcgcc gcaatcgcat cccaatccgg tctcataaac gagcggacag gtgggcgggt
     2701 ggctttcatc aaaaccggcg ggacaatgcc gataacctct ttgttgcgtg ccttctgtac
     2761 cgcgttctgc gtggttgcct tcggcttggc gggcagcgcg gcctacgccc aaagcggcaa
     2821 tggcaccccc ccggaacagc aaaagtcggc cgtcgacgcc gccaaagagg gccaaaaaag
     2881 catcgacgag atcgccgagg cctcgcgcct gctcaccggg ccggccggca atcccgaatg
     2941 cgtatggctc ggccgccggg tggtcagcct gctgtggcgc gacgatctcg acaccgcctt
     3001 ccgccacctc gatctttacg accgttttgg ctgccccagc tcgcatatcc aggccacatt
     3061 ccgctgtctg gtgcgccagg gccatatcga cccgaaggcc caggacagcc tgaacgggcg
     3121 tgtccatctc tgctggctca atccggcatt gaccccggcc ccggcggcag ccgccgccca
     3181 gccgccggcc gccgccaccg cgacctcggg cggaacgact ccgcactgat cgcgtttcct
     3241 ccaagcaaat attcttatcg cggtcatgcg cgtgacaagg ttttgccatg tccggttcgt
     3301 acttgaataa aggcctgaag ccgggctatg ctgcattgca acagatcaag cctcggctga
     3361 acccagggga cgggttcgaa ggtctgccat ccagccccaa ttggttgtga gtgttatgcg
     3421 caccgtcgct gccgtgatcg ccctcgtggc gtgcgtgcaa gcaggtttgt gggctgctgg
     3481 ccaggatcac ctgagcgcgc ccaacatcga tggccaattc gccagcgttt cctatgcgcc
     3541 gttcgcgagt tccgttaatc ccgatgaagg cggcaaagcg caggaagcgc aaattcgcgc
     3601 cgacctgcag aaactcgcgc cgctgacgcg cgcgatccgc acttattcgt ccaccggcgg
     3661 cgtcgaactt gttcccggca tcgccgccga attcgggttg cgcgcgacga tcggcgcctg
     3721 gatcgacaaa aatcaggacc gcaacgagcg cgaaatccgc tcggtggtcg aactcgcgaa
     3781 gcgtcatagc aacgtcaacg gcatcatcgt cggcaacgaa acgatttatc gcggcgagtt
     3841 gaaggttccc gaactgatcc agttgatcca gcgcgtcaag cgtcagagca acgtcccggt
     3901 caccaccggt gaaatctgga atgtctggat cgagcacccg gagctggtgt cgtcggtcga
     3961 ttacatcgcc gcgcacatcc tgccttattg ggaaggtttc tccgaaggca aggctgtcga
     4021 ccaggcgatc ataatttacg acaaacttcg gcaagcctat cccggcaagc gcatcgtgat
     4081 cgccgaattc ggctggccga gcgcgggcta caacatgcac aacgccaatc ccggccgcgt
     4141 cgagcaggcg gtcgtgctgc gcgacttcat ttcgcgcgcc gaggcgctcg gcatcgacta
     4201 caacatcgtc gaagcgatcg accagccgtg gaaaatcttc gaaggcggcg tcggacccta
     4261 ttggggcctg ctcgacgcat cgcgccatca gaaattcgaa tggaccggtc cgatcaccga
     4321 tccggatcac tggaagctcg ccggcatcgc gctgctggtg agcgtgctgc tgtccttgcc
     4381 aatcctggcg atgaccgcgg cgtcgttctg gcagaccgtc atgctcgccg ccgccgccaa
     4441 tgtggtgggc gcgtggttcg ccgcggtgtt tgcctattgg aacggacatt attttgtgcc
     4501 gggcgctgcc ttcgcccttg gccttggcgt cgtgctgctg atcccgctgg tcatcatcgc
     4561 actggcgcgc atcgatgagc tcgccatgat cgccttcggc cgcaagccgc agcggctggt
     4621 cggctcgcca tcgctggtgc cacagttgcc cgaggcagcg actccgaaag tctcgatcca
     4681 cctcccggcc tataacgagc cgccggagat gctcaagcag acactcgatg cgatggcgcg
     4741 gctcgattat ccgaatttcg agtgcgtggt ggtgatcaac aacacgcccg atccggcttt
     4801 gtggcggccc atcgaagagc actgccaagc actcggcgaa cgattcaagt tcgtcaacgc
     4861 cgacactttg cagggcttca aggccggcgc gctgcgactt gccatggtgc acaccgcggc
     4921 cgatgccgaa gtgatcggtg tgctcgacgc cgactatgtt gtgcagccga actggctcaa
     4981 agatctcgtg ccgctgttcc gcgacgctac ggtgggtctg gtgcagg
//
LOCUS       DASWZT010000210        13868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_83703, whole genome shotgun sequence.
ACCESSION   DASWZT010000210 DASWZT010000000
VERSION     DASWZT010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13868
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83703"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VGE92_05710"
     CDS             complement(<1..886)
                     /locus_tag="VGE92_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY1313347.1"
                     /translation="MFKRLRGYFSNDLSIDLGTANTLIYVRERGIVLNEPSVVAVQDE
                     PTRGGKIIVAVGVEAKNMLGRTPGHITAVRPMKDGVIADFTYTEKMLQYFIKKVHGNR
                     FLSPSPRVLVCVPFGSTQVERRAIKESAEGAGARSVDIVPEPMAAAVGAGLPVQEARG
                     SMVLDIGGGTSEVAVISLNGIVYAASVRIGGDKFDEAITNYVRRNYGILIGEATAERI
                     KIEIGSAYPGQQVRELSVKGRNLSEGVPRSFTLNSNEILEALQEPLQSIVSAVKQALE
                     QTPPELGADVADRGIVLTG"
     gene            1022..1312
                     /gene="gatC"
                     /locus_tag="VGE92_05715"
     CDS             1022..1312
                     /gene="gatC"
                     /locus_tag="VGE92_05715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEY1313348.1"
                     /translation="MSLTRQDVEKIARLARLSITEEEMPVYVTSLSSIVNFVDELARA
                     HTDQVEPMAHPLDGQHQRLRADVVSESDHHEKYQSNAPAVQAGLYVVPRVIE"
     gene            1325..2779
                     /gene="gatA"
                     /locus_tag="VGE92_05720"
     CDS             1325..2779
                     /gene="gatA"
                     /locus_tag="VGE92_05720"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEY1313349.1"
                     /translation="MLHTFSIGELARRLSAKEISSVELTRHFLARIERLNPALNAVIT
                     LTGDAALAAAALADARRARGEDGPLLGIPLIHKDIFCTDGVRTSCGSRMLDNFIAPYD
                     ATVVARLKADGAVMLGKANMDEFAMGSSNETSYYGPVKNPWDRTRVPGGSSGGSAAAT
                     AARLAPFATGTDTGGSIRQPAALTGVTGVKPTYGRVSRYGMVAFASSLDQAGVFARSA
                     ADAALVLQSMAGFDARDSTSIDTPVPDYSAALGAPLAGLKIGLLREFFEGLEARNAAL
                     IHDALQVYRELGATTVEVSLPHLPMSVPTYYVVAPAECSSNLSRFDGVRYGYRCENPK
                     DLLDLYTRSRGEGFGAEVKRRIMTGTYVLSAGYFDAYYLKAQKVRRLITDDFRAAFTQ
                     ADLLIGPTTPTPAFAIGAKIDDPVTMYLNDIYTIGANLAGLPGLSLPCGYVDALPMGL
                     QLIGPHFSEAKLLNAAHRFQEATEWHRHAPRGYE"
     gene            2746..4227
                     /gene="gatB"
                     /locus_tag="VGE92_05725"
     CDS             2746..4227
                     /gene="gatB"
                     /locus_tag="VGE92_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005247018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEY1313350.1"
                     /translation="MAPARAARVRVMSDWEVVIGLEIHAQLATKSKIFSGSATAYGAP
                     PNSQADLVDLAYPGVLPVLNAQAVRMAVKFGLSIGAEVAHHSVFARKNYFYPDLPKGY
                     QISQYELPVVKQGKLSVTLEDGSVKVIGITRAHLEEDAGKSLHEGLAHASGIDLNRAG
                     TPLLEIVSEPDMRSAKEAVAYMKKIHTLVRYLEICDGNMQEGSFRCDANVSVRPRGRE
                     KFGTRAEIKNLNSFRFVEKAIHYEVARQVELLESGGSVVQETRLYDSDKDETRSMRSK
                     EEANDYRYFPDPDLLPVQIDATFIEAVRATLPELPDAKAARFAHDFGLSPYDAGVLSA
                     SRELGAYFEAVVAESGADHAKLAANWVMGELSSALNRDAVEITAARVTPLQLGGLLRR
                     IVDQTISGKIAKEVFEAMWSDGKSADAIIEAKGLKQITDSGAIDSVIDAVIAANPKQL
                     ADYRAGKDKLFGFFVGQVMKATEGKANPAQLNELLKRKLGGGA"
     gene            4229..5122
                     /locus_tag="VGE92_05730"
     CDS             4229..5122
                     /locus_tag="VGE92_05730"
                     /inference="COORDINATES: protein motif:HMM:NF013589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEY1313351.1"
                     /translation="MHDRDCLHRFMFEHYPIRGHLVHLDAAWAALIEHRDYPPAIRDT
                     LGETVAAALLLAATVKFEGVLSLQLQGDGAVHLLLAQCTNGLGVRGLARYREAGGGAL
                     LGTGNLTVTVETDDGAQRYQGIVPISGRRVAESLQVYFENSEQLPTRLWLHADASGVS
                     GMLLQKLPNGAAQHSAAAPGVAAIDDAWRRVQLIGETLTPDELRTLQDVEILHRLFNE
                     DDVRVFEPAPVYFRCRCSRERVAGMLQGLGETETRSVLAERGEVEVRCDFCNRAYVFD
                     AIDVAQLFNAHVAGHAGRSTH"
     gene            5136..6356
                     /locus_tag="VGE92_05735"
     CDS             5136..6356
                     /locus_tag="VGE92_05735"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEY1313352.1"
                     /translation="MNFRLPGFMLGIGIVAAARGGAASDAPFYANSFPRVPPVAAMTA
                     IGRALFSDPALSASGTVACASCHDPKHAYGPPNDLPVQRAGRDAGRFGVRAVPALTYT
                     QNIPPFGEHYFDDEGDDSVDQGPAGGRTWDGRAQSAHEQARLPLFSPFEMANSSSAAI
                     VGKVRAAAYAPQLREAFGATLFEDGALAFKAILLALETFQQDAAEFYPYSSKYDAWLR
                     HETALSREELQGLALFNDSQKGNCARCHPSSVRGGAFPQFTDFGYAAVGVPRNRAIPA
                     NDDPRYFDLGLCGPWRTDLRERTEYCGMFRTPSLRNVAMRRAFFHNGGVHRLEDAVRF
                     YAERDTQPQKWYPRGRDGVPRKFDDLPPAYQGNIDEQAPFGRHPGEVAALSAADIKHL
                     VAFLETLTDGYRSK"
     gene            complement(6361..7683)
                     /locus_tag="VGE92_05740"
     CDS             complement(6361..7683)
                     /locus_tag="VGE92_05740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY1313353.1"
                     /translation="MSAETCDVAVIGGGPSGSTAAALLARRGYAVIALEKARHPRFHI
                     GESLLPMNLPIFERLGVLDKVRAMGVFKPGADFEADNARGYNTYAFARAIGQSPPHAY
                     QVWRQDFDQMLYQHARECGADAREGHEVTAAVHLGPRQSRLEVRTDDARNYAIEARYV
                     IDASGRDAFLASKKRLRRKNDRHQSAAIFAHFHGAERRRGEDEGNISIYSFEHGWMWM
                     IPLPRGVMSVGAVCRPDYLKQRKGRTMEFLLDTLKRNPALWRRLEHAELIGEEVRVTG
                     NYSYDSLQMGAPGWVLVGDAFAFLDPVFSSGVYLAMSGAEQAAGVVDAALREPARELS
                     LLRKFEKRQRAGMARFSFFIYRFNGPIMQQMFRAPRNTWQLEQGVISMLAGDLFDTPQ
                     VLWRLNLFKLVYALCGLRDWRRWRAEHRYRLQQARSQFTGGNTPLDKL"
     gene            complement(7731..9128)
                     /locus_tag="VGE92_05745"
     CDS             complement(7731..9128)
                     /locus_tag="VGE92_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313354.1"
                     /translation="MKFAPRRTALLAIMVAPTLSWAAPPMSPDTGSDAVRALHSQRAG
                     ELELQYRREVRGASTDQVSFGIAADYHYVKSADGTRIYDYRSRRIFWLRPAGEFVNNS
                     LYADVWFRAAELKNRSALGGMLNGAGIAASKAPGMTVPFWAETELGMLSADMPRSDLK
                     TVEEESRVRWLLDADEVAAVRYERDPVPDTVKSGLRRFWRTVVQLHPDIADALAASGR
                     MPAELWIKSMPPGKDPLVAHWTLAAKRWEPAAAYPLPAHLTAQPTNSDAVFSRIFATL
                     AMNVAAKATPPSAEVYVARAESAIARRAGLEAMLWAIEMNLAQGTPISQCVEKDPRPY
                     CSLLVRAGPLAKADPRTALGFTKQAPAEADRAQFAALPNAYLLRLLWATRPPGAGVDR
                     KENERDLLAALEASPVANFCKDTGDFYAGSWQPFAAWQAWDFGRAMAGHRSGDLLGGV
                     DALEASLASQEALFF"
     gene            complement(9139..10065)
                     /locus_tag="VGE92_05750"
     CDS             complement(9139..10065)
                     /locus_tag="VGE92_05750"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY1313355.1"
                     /translation="MSSTALELRHLETLLALAECGSLSKAAARLFLTQSALSHQLKAL
                     ESHYGAALVEKNIRPLRFTAIGQRLLTLARTVLPQVAEAGRDVARLAQGHSGPLRVAV
                     QCHNCFDWLMPAMDAYRTLWPEVELDIISGFVVDPLPLLDRGEAELAIIHDPQGPHPN
                     VVFSPLFRYESVALMSPRHRLAAKGWLEAADFTQETLITYPVPDEMLDVMKHCLTPAG
                     INPKRRTAELTVAILQLVASGRGIAALPSWTVGNYIERGYVVSRPIGPSGLHCELYAA
                     TTDRGAEAAYIREFIALTRAQSLTELAGVSAL"
     gene            10163..11050
                     /gene="metF"
                     /locus_tag="VGE92_05755"
     CDS             10163..11050
                     /gene="metF"
                     /locus_tag="VGE92_05755"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEY1313356.1"
                     /translation="MSNPVAVSFEFFPPSDEPMGRQLWDCVQRLAPLQPKFVSVTYGA
                     DGSTRARTHECVLRILRETELVVAPHLTCVGAARSEVMKIAEDYWQQGVRHLVALRGD
                     AAAANRTPEGRYRPHAGGFAYASDLVAGLTGVGRFDVSVAAFPEGHPESGGIGADIEN
                     LKRKLDAGANRAITQFFFEPDVFLRYRDRCAAAGIRADIVPGILPITRFPQMLRFAER
                     CGTTVPQWLRQRFDGLDDDPETRRMIAASVAIEQVQRLREHGVDEFHFYTLNRAELSY
                     AICHSLGLRPHVPAEAQVA"
     gene            11052..>13868
                     /gene="metH"
                     /locus_tag="VGE92_05760"
     CDS             11052..>13868
                     /gene="metH"
                     /locus_tag="VGE92_05760"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF007024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEY1313357.1"
                     /translation="MPIESQVARGAAFLTLLRERLVILDGAMGTMIQRHALTEAQFRG
                     ERFAAWACDLRGNNDLLSISQPDIIAAIHRDYLLAGADVISTNTFNSTAISQADYAMQ
                     SLAVELNTTAARLARRVADEISAATGQQRFVAGALGPTSRTASLSPDVNDPGFRNVSF
                     DELVSAYSAATRALIDGGTDIILIETVFDTLNAKAALYAVRTVLDEAGLDLPLIVSGT
                     ISDASGRTLSGQTTEAFWNSIRHARPAVVGLNCALGGRQLRPYIEEMARIADTYVCAY
                     PNAGLPNAFGEYDETAHETAAILAEFAASGFVNMVGGCCGTTPEHIRQMARAVAGIAP
                     RALPSIAPACRLAGLEPLTIDADSLFVNVGERTNVTGSAKFRKLIEANDYPAAVDIAR
                     QQVANGAQIIDVNMDEGMLDSQAAMAKYLSLIAGEPDIARVPVMIDSSKWSVIEAGLK
                     CVQGKPIVNSISLKEGEEAFLAHARQCMRYGAAVVVMAFDERGQADTIDRKVAICERA
                     YRLLTERLGFMAADIIFDPNIFAVATGIEEHNRYSLDFIEATAAIKQRMPGVHVSGGV
                     SNVSFSFRGNDPVREAMHSVFLYHAIRAGLTMGIVNAGQLAIYEDIPTELRERCEDVI
                     LARRADATERLLEIAQSFKGGGAQKRGDDLEWRNLPVAERLQHALIKGLDEFVLQDTE
                     EARLQAKRPLDVIEGPLMEGMNVVGDLFGAGKMFLPQVVKSARVMKKAVSVLVPYIEK
                     EKDGSSRSNGKVVMATVKGDVHDIGKNIVGVVLQCNNFEVLDLGVMVSCEKILETATR
                     EGASLIGLSGLITPSLDEMVHVAKEMQRLGYRQPLLIGGATTSPAHTAVKIDPQYQGA
                     VVYVKDASRSVGVCQQLVTEGTRDAYIATLKAEHARRREQHRNKGVKAPRLSLLQARA
                     KRLHIDWAGYVPPVPSFLGVRAFDD"
BASE COUNT         2493 a         4366 c         4526 g         2483 t
ORIGIN      
        1 cgccggtgag cacgatgccg cgatcggcga cgtccgcgcc gagttcggga ggagtctgtt
       61 cgagcgcctg cttgaccgcg ctcacgatgc tctgcagggg ctcctgcagg gcctcgagga
      121 tctcgttgct gttcagggtg aagctgcgcg gcacgccctc ggaaagattg cgtcccttca
      181 cggacagctc gcgcacctgc tggcccgggt atgccgagcc gatctcgatc ttgatgcgct
      241 cggcagtggc ctcgccgatc agaatgccgt agttgcggcg cacgtagttg gtgatcgcct
      301 cgtcgaactt gtcgccgccg atgcgtacgc tcgccgcgta gacgatgccg ttcagcgaga
      361 tgacggcgac ctccgaggtg ccgccgccga tgtcgagcac catcgaaccc cgcgcttcct
      421 gcaccggcag cccggcaccg accgccgccg ccatcggctc ggggacgatg tcgacgctgc
      481 gcgcgccggc accttcggcc gactccttga tcgcgcgccg ctccacctgg gtggagccaa
      541 acggcacaca caccagaacc cgcgggctcg ggctcagaaa acgattgcca tgcaccttct
      601 tgatgaagta ctgcagcatt ttttccgtgt aggtgaaatc cgcaatgacc ccgtccttca
      661 tcggccgcac ggcggtgatg tggccagggg tgcgccccag catgttcttg gcttcgaccc
      721 cgaccgcgac gatgatcttg ccgccgcgcg tcggttcatc ctgcaccgcg acgaccgagg
      781 gttcgttgag aacaatgccc cgttcgcgga cgtaaatcag cgtattggcc gtccccagat
      841 cgattgagag gtcattggag aaatagcctc taagacgctt gaacataggg tattttttct
      901 ggcaaagggg ggtgaaaaaa agttgcgcta atgtaacaac cgacacgaca ttcagcaagg
      961 caacgtgtat cattggccgg gcttattttg cgtgagatgg cacgcacttt tgggcactcc
     1021 tatgagccta acccgtcaag atgtggagaa gatcgcaagg ctcgcgcggc tgtcgatcac
     1081 cgaagaagag atgccggtct acgtgaccag tttgtcgagc atcgtcaatt tcgtcgacga
     1141 attagcgcgg gcacacaccg atcaggtcga gcccatggcg catcctctgg atggccagca
     1201 tcagcggctg cgcgcggacg tcgtgtcgga gagcgaccac cacgagaaat atcagagcaa
     1261 cgcgccggcg gtgcaagccg gcttgtacgt ggtaccgcgg gtcatcgagt aggtccgcga
     1321 ttcgatgttg cacacatttt ccataggcga actcgcccgc cgcctgagcg cgaaagagat
     1381 ttcgagcgtc gaactgacgc ggcatttcct ggcgcgcatc gagcgcctga acccggcgct
     1441 gaacgcagtc atcaccctca ccggcgatgc ggccctcgcc gcagcggcgc ttgccgatgc
     1501 gcgccgagcc cgcggcgagg acggcccgct gctgggcatc cctctcattc ataaagatat
     1561 tttctgcacc gacggcgtgc gcaccagttg tggttcgcgc atgctggaca atttcatagc
     1621 gccctacgat gcaaccgtgg tggcgcgcct caaggcggac ggcgccgtca tgttgggcaa
     1681 ggccaatatg gacgagttcg ccatgggatc ctccaacgag accagttatt acggaccggt
     1741 caagaacccc tgggaccgaa ctcgtgtgcc cggcggatca tcgggtggtt cggccgcggc
     1801 gacggccgca cgtctggcgc ccttcgccac cggcaccgat accggcggtt cgattcggca
     1861 gcccgcggca ttgaccggcg taaccggcgt caagccgacg tatggacgcg tgtcgcgcta
     1921 cgggatggtc gctttcgcat cgagtctcga tcaggcggga gtattcgcgc gcagcgccgc
     1981 ggatgcggcg ctcgtcttgc aaagcatggc gggattcgat gcgcgcgatt ccaccagcat
     2041 cgacacgccg gtaccggatt actccgcggc actcggcgcg ccgctcgccg gattgaagat
     2101 cggactgctg cgcgagttct tcgaaggctt ggaggcgcgc aatgcggcgt tgattcacga
     2161 cgcgttgcag gtgtatcgcg agctcggcgc gacgaccgtg gaggtcagcc tgccgcatct
     2221 gccgatgtcg gtgcccacct attacgtggt cgcgccggcc gagtgctcgt ccaatctgtc
     2281 gcgcttcgac ggcgtgcgtt acggctatcg atgcgagaat cccaaggatt tgctggatct
     2341 ttatacgcgc tcgcgcggcg aaggtttcgg tgccgaggtc aaacgccgca tcatgaccgg
     2401 cacgtacgtg ttgtcggccg gctacttcga tgcgtattat ctaaaggcac agaaggtgcg
     2461 ccgcctgatc accgacgact ttcgcgcggc tttcacgcag gcggatctcc tgatcgggcc
     2521 gacgacgccg acgcctgcct tcgcgatcgg cgccaagatc gacgatccgg tcaccatgta
     2581 tttgaacgac atctatacca tcggcgcgaa cctcgccggt ctgcccgggc tgtcattgcc
     2641 ctgcggctac gtcgacgcgt tgccgatggg cttgcagctc atcgggccgc acttctccga
     2701 ggcgaaactc ttgaatgcgg cgcatcggtt ccaagaagcc accgaatggc accggcacgc
     2761 gccgcgcggg tacgagtaat gagcgattgg gaagtcgtca tcgggctcga gattcacgcg
     2821 cagctcgcca cgaagtcgaa gatattctcc ggcagcgcga ccgcgtatgg tgcgccgccc
     2881 aacagccagg ccgacttggt cgatttggcc tatcccggcg tgttgccggt gctgaatgcg
     2941 caagccgtgc gcatggcggt caaattcggc ctgagcatcg gtgccgaggt cgcgcaccat
     3001 tcggtgttcg cgcgcaaaaa ttacttttat cccgacctgc cgaagggcta tcagatcagc
     3061 cagtatgagc tgcccgtggt caagcagggg aaactctccg tgacgctcga agacggcagc
     3121 gtcaaggtga tcggcatcac ccgcgcgcat ctcgaagagg acgccggcaa gtcactgcac
     3181 gaagggctgg cgcacgcctc gggcatcgat ttgaatcgcg ccggcacgcc gctgctcgag
     3241 atcgtctccg agcccgacat gcgctccgcg aaagaggcgg tcgcctacat gaagaaaatc
     3301 cacaccctgg tgcgctacct cgagatctgc gacggcaaca tgcaggaggg gtcgttccgt
     3361 tgcgacgcga acgtgtcggt tcggccgcgc ggccgcgaga aattcggcac gcgcgcggaa
     3421 atcaagaatt tgaactcgtt tcgtttcgtc gaaaaggcga ttcactacga agtcgcgcgc
     3481 caggtcgagt tgctcgagag cggcgggagc gtggtgcagg agacgcgcct gtacgattcg
     3541 gacaaggacg agacgcgttc catgcgctcc aaggaggaag ccaacgatta ccgctatttc
     3601 ccggatccgg atctgttgcc ggtgcagatc gacgcgacct tcatcgaagc cgtgcgcgcg
     3661 acgctgccgg agttgccgga cgccaaggcg gcgcggtttg cgcacgactt cgggctctcg
     3721 ccctacgatg ccggtgtgtt gagcgcgagc cgcgagctcg gcgcctattt cgaggccgta
     3781 gtcgccgagt cgggcgccga ccacgcgaaa ctggccgcca attgggtgat gggcgagctc
     3841 tcgagcgcac tcaatcgcga cgccgtcgag atcacggcgg cccgggtcac gccgctgcaa
     3901 ctgggcggct tgttgcggcg gatcgtcgat cagaccatct cgggaaaaat cgccaaagaa
     3961 gtgttcgagg ccatgtggag cgacggaaaa tccgcggacg ccatcatcga ggccaagggc
     4021 ctgaaacaga tcaccgactc gggcgccatc gacagcgtga tcgacgcggt gattgccgcc
     4081 aaccccaagc agctcgccga ctatcgcgcc ggcaaggaca aattgttcgg cttcttcgtc
     4141 ggacaagtga tgaaggcgac cgagggcaag gcgaatcccg cgcaactcaa cgaattgctg
     4201 aaacgcaaac tcgggggtgg cgcttaagat gcatgaccgc gactgcctgc atcgcttcat
     4261 gttcgagcat tacccgattc gcggtcactt ggtacacctg gatgcggctt gggctgcgtt
     4321 gatcgagcat cgcgattacc cgccggcgat ccgcgacacg ctcggtgaaa cggtggccgc
     4381 cgcgctgctg ctggcggcaa cggtcaagtt cgagggcgtc ttgtcgctgc aattgcaggg
     4441 cgacggcgcg gtgcacctgc tgctcgcgca atgtaccaac ggtctcgggg tgcgcggcct
     4501 cgcgcgctac cgcgaagcgg ggggtggcgc actgctcggc accggcaacc tcacggtgac
     4561 cgtggagacc gacgacggtg cgcagcgcta tcaaggcatc gtgccgatca gcggccggcg
     4621 agtggccgaa tcgctgcagg tctacttcga gaattccgag caattgccga cccggctgtg
     4681 gctgcatgcc gatgcgtccg gcgtctcggg gatgctgctg caaaagcttc ccaacggcgc
     4741 tgcgcagcac agcgccgcag cccccggcgt cgcggccatc gacgacgcct ggcgccgggt
     4801 gcagctgatc ggcgagactc tcacgccgga tgagctgcgc accttgcaag acgtcgaaat
     4861 cctgcaccgc ttgttcaatg aggacgatgt gcgcgtgttc gaaccggcac cggtgtattt
     4921 tcgttgccgc tgctcgcgcg aacgggtggc cggcatgttg cagggcctgg gtgaaacgga
     4981 aacccgttcg gtactcgccg agcgcggcga agtcgaggtg cgttgcgact tctgcaaccg
     5041 ggcttacgtc ttcgacgcga tcgatgtggc gcagttgttc aacgcacacg tcgccggcca
     5101 tgccggtcgc tcgactcact gaagttcggg cggcgatgaa tttccggctc ccagggttca
     5161 tgctcgggat cgggatcgtt gcggcggcac gcggcggcgc cgcgtcggat gcaccgttct
     5221 atgccaacag tttcccgaga gttccgccgg tcgccgccat gacggcgatt gggcgtgcgc
     5281 tgttctccga tccggcgttg tcggcctcgg gcacggtggc ctgtgcgagc tgccacgatc
     5341 cgaagcacgc ctacgggccg ccgaacgact tgccggtgca gcgcgcgggc cgcgatgccg
     5401 gccgcttcgg ggtacgcgcg gtgcccgcgc tcacgtacac acaaaatata ccgccgttcg
     5461 gcgagcacta tttcgacgat gagggcgacg acagcgtcga tcaggggccg gccggtggcc
     5521 gcacctggga cggccgggcg cagtcggctc atgagcaggc gcgcctgccg ctgttctcgc
     5581 ctttcgaaat ggccaactcg agcagcgcgg ccatcgtcgg caaggtccgc gccgccgcct
     5641 acgcgccgca gctgcgcgag gcgttcggcg caacgctgtt cgaggacggc gcattggcgt
     5701 tcaaggccat tctgctggcc ttggagacgt ttcagcaaga cgccgccgag ttctatccct
     5761 acagcagcaa gtatgacgcg tggctgcgcc acgagaccgc gctaagccgc gaggaattgc
     5821 agggcttggc gctcttcaac gattcgcaga agggcaactg tgcccgctgt catccgagca
     5881 gcgtgcgcgg cggcgcgttt ccgcagttca cggatttcgg ctacgcagcc gtcggcgtgc
     5941 cgcgcaaccg cgccatcccc gccaatgacg atccgcgcta tttcgatttg ggtttatgcg
     6001 gaccctggcg caccgatttg cgggagcgaa ccgagtactg cgggatgttc cgtacgccga
     6061 gcctacgcaa tgtggcgatg cgccgcgcgt tttttcacaa cggcggggtg catcgtctgg
     6121 aagacgccgt gcgtttctat gcggaacgcg acacccagcc gcagaagtgg tatccgcgcg
     6181 gcagggacgg ggtgccgcgc aaattcgacg atctgccgcc ggcttatcag ggcaacatcg
     6241 atgagcaagc gcccttcggc cgacacccgg gtgaggttgc agcgttgagc gcggccgaca
     6301 ttaaacacct ggtggccttc ctcgaaacgc tgaccgacgg ctatcgatcg aagtagcctc
     6361 ttagagcttg tcgagcgggg tgttgccgcc cgtgaattgc gagcgagctt gctgcagccg
     6421 atagcggtgc tcggcgcgcc agcgccgcca atcgcgcagg ccgcacagcg catacaccag
     6481 cttgaacaaa ttcagccgcc acagcacttg cggggtgtcg aacagatcgc cggccagcat
     6541 ggagatcact ccctgctcga gctgccaggt attgcgcggc gcgcgaaaca tctgttgcat
     6601 gatcgggccg ttgaagcgat aaatgaaaaa cgagaatcgc gccatcccgg cgcgctggcg
     6661 cttttcgaat ttgcgcaaca gcgacaactc ccgggccggc tcacgcaacg ccgcatccac
     6721 gaccccggct gcctgctccg cgccgctcat cgcgaggtat acaccggagg aaaaaaccgg
     6781 atcgagaaac gcgaatgcgt cgcccaccag cacccatccg ggagccccca tctgcagcga
     6841 gtcgtaggaa tagttgccgg tgacacgcac ctcctcgccg atcagttcgg cgtgctcgag
     6901 gcggcgccac agcgcgggat tgcgcttgag cgtgtcgagc aagaactcca tcgttcggcc
     6961 cttgcgctgc ttgagataat cgggacggca caccgcgccg acgctcatca ccccccgcgg
     7021 cagcggaatc atccacatcc agccgtgttc gaagctgtag atgctgatgt tgccttcatc
     7081 ctcgccccgg cggcgttcgg cgccgtgaaa atgagcgaaa atggcggcgc tttgatgccg
     7141 atcgttcttg cgccgcagcc tcttcttgga agcgaggaat gcgtcgcggc cgctcgcatc
     7201 gattacgtag cgagcctcga tcgcgtaatt gcgggcgtca tcggtacgca cctcgagccg
     7261 cgattgccgc gggccgagat gcacggccgc agtcacctca tggccttcgc gcgcatcggc
     7321 gccgcactcg cgggcatgct ggtagagcat ctggtcgaaa tcctggcgcc acacctggta
     7381 ggcgtgcggc ggactctggc cgatggcgcg cgcgaaggcg taggtgttgt agccgcgcgc
     7441 attgtccgcc tcgaaatccg cgccgggctt gaaaaccccc atggcgcgga ccttgtcgag
     7501 cacgccgagc cgttcgaaga tcggcagatt catcggcaac agcgattcgc cgatgtgaaa
     7561 gcgcgggtgg cgggctttct ccagggcgat gaccgcatag ccgcggcgtg ccaacaacgc
     7621 ggccgccgtg ctgccgctcg gaccaccgcc gatcaccgcg acatcgcagg tctcggcgct
     7681 cattgttgtc gtttgcgcat gtcataggcc ttttttgggc gtagtggcaa tcagaagaat
     7741 aacgcctctt gcgaggcgag gctcgcctcg agcgcgtcga cgccgcccaa caaatcgccg
     7801 ctgcgatggc ccgccatggc acgtccgaaa tcccatgctt gccaggcggc gaagggttgc
     7861 caggagccgg catagaaatc gcccgtgtcc ttgcagaagt ttgccaccgg gcttgcctcg
     7921 agcgcggcga gcaggtcgcg ctcgttttcc ttgcggtcga ctccggcgcc gggcggccgg
     7981 gtggcccaca gcagccgcaa caggtacgca tttggcagag cggcaaactg cgctcgatcg
     8041 gcttcggcgg gagcttgttt cgtgaatccc agcgcggtgc gtggatccgc ctttgccaaa
     8101 ggaccggccc gaaccagcag agaacagtag ggacgcgggt ctttttccac gcattgggaa
     8161 atcggcgtcc cttgtgcgag attcatctcg atcgcccaca gcatggcctc gaggccggca
     8221 cgcctcgcga tcgcggactc ggcgcgcgcc acgtacacct cggcggaggg cggggtcgct
     8281 ttcgcggcca cgttcatcgc aagcgtcgcg aaaatcctgg agaacaccgc atcggagttc
     8341 gtcggctggg cggtcagatg cgccggcagc gggtacgccg cggccggctc ccagcgcttc
     8401 gccgcgagcg tccagtgcgc gacgagcgga tccttgccgg gcggcatcga ttttatccaa
     8461 agctccgccg gcattcggcc gctcgccgcc agggcatcgg cgatgtccgg gtgcagttgg
     8521 acgacggtgc gccaaaagcg ccgcaatccg gacttcacgg tgtcgggtac ggggtcccgc
     8581 tcgtagcgca cagccgccac ttcatcggcg tcgagcagcc aacgcacgcg cgattcttcc
     8641 tccacggtct tgaggtcgct gcgcggcata tcagcggaga gcatcccgag ttcggtttcg
     8701 gcccaaaagg gaacggtcat gccgggcgcc ttcgacgctg cgatgcccgc gccattgagc
     8761 atcccgccga gcgcggaacg attcttgagt tccgcggccc gaaaccagac atcggcgtac
     8821 agggagttgt tgacgaattc acccgcggga cgcaaccaga aaatgcgccg cgaccggtag
     8881 tcgtaaattc gcgtaccgtc ggccgacttc acgtagtggt agtcggctgc gatgccgaac
     8941 gagacttgat cggtgctggc gcctcgaacc tcgcgtcgat attgcaattc gagttcgcct
     9001 gcgcgctgcg aatgcagcgc ccgcacggca tcgctgcccg tgtccggtga catcggcggc
     9061 gccgcccaag agagcgtcgg ggcaaccatg atcgcaagca gcgcggtcct gcgcggggcg
     9121 aatttcaaaa tcggtctcct agagtgccga tacgccggcc aactcggtca ggctttgggc
     9181 tcttgtgagg gcaatgaact ctcttatata ggcggcctcg gcaccgcgat cggtcgttgc
     9241 cgcgtacagc tcgcaatgca ggccgctcgg gccaatcggg cgcgacacga catagccgcg
     9301 ctcgatgtag ttgcccacgg tccaggacgg cagggcggca atgccgcggc cgctcgccac
     9361 cagctgcagg atggccaccg tgagctcggc ggtgcgccgt tttggattga ttccggccgg
     9421 cgtcaaacag tgcttcatca catcgagcat ctcgtccggt accgggtagg tgatgagcgt
     9481 ctcctgcgtg aaatcggcag cctcgagcca gcccttggcg gccaggcgat gccgtgggct
     9541 catgagagcc acgctctcgt agcgaaataa cgggctgaac accacgttgg gatgcggacc
     9601 ttgcggatcg tgaatgatgg caagttctgc ctcgccgcga tcgaggaggg gcaaaggatc
     9661 gacgacgaaa cccgaaatga tgtcgagttc gacttcgggc cagagcgtgc ggtacgcatc
     9721 catggccggc atgagccagt cgaagcagtt atggcattgc accgcaacac gcaagggtcc
     9781 ggagtggccc tgggccaggc gggccacgtc gcgtcccgcc tcggcgacct gcggtagcac
     9841 ggtccgcgcc agagtgagca agcgctggcc gatggccgtg aagcgcaagg ggcggatgtt
     9901 tttctcgacg agcgcggctc catagtgact ttccagcgct ttgagctggt gcgacagcgc
     9961 cgactgggtc aggaacagcc gcgccgccgc cttggaaagg ctgccgcact ccgccaacgc
    10021 caatagcgtc tccaagtgtc gaagctccaa tgcggtggac gacatgagct caattcatcc
    10081 tctatatgag aataatgagt ttggatcata gcctaggtcg atgcatgctt gcagtcgaga
    10141 tcttccgcct tgaggaattg ccatgtcgaa tccggtggcc gtgtcgttcg agttctttcc
    10201 gcccagcgat gagccgatgg ggcgacagct gtgggattgc gtacagcggc tcgccccgct
    10261 gcaaccgaaa ttcgtgtcgg tgacctatgg cgccgacggc tcgacgcgcg cccgaacgca
    10321 cgagtgcgtg ctcagaatcc tgcgcgagac cgagctcgtg gtcgcgccgc atctcacctg
    10381 cgtcggcgcc gcgcgctccg aggtgatgaa aatcgccgag gactattggc agcagggcgt
    10441 gcggcatttg gtggcgctgc gcggcgatgc agcggcggcc aaccggacgc cggagggacg
    10501 ctatcgccct catgccggcg gatttgcgta tgcctccgac ttggtcgcag ggctcaccgg
    10561 tgtcggaaga ttcgatgtat cggtggcggc gttcccggaa gggcatccgg agtccggcgg
    10621 catcggcgcc gacatcgaga atctgaagcg caagctcgac gccggcgcga atcgggcgat
    10681 cacgcagttt tttttcgaac ccgatgtgtt cctgcgctat cgggaccgct gcgccgcggc
    10741 cggcattcgc gccgacatcg taccggggat tttgccgatt acccgctttc cgcagatgct
    10801 gcgcttcgcc gaacgttgcg ggaccacggt tccgcagtgg ttgcgccagc gcttcgacgg
    10861 actcgacgat gacccggaaa cgcggcgcat gattgccgcg agcgttgcca tcgaacaggt
    10921 gcagcgcctg cgcgagcacg gcgtcgacga atttcatttc tatacgctga atcgcgcgga
    10981 gctcagctac gccatttgcc acagtttggg gttgcggccc cacgtgcccg ccgaagcgca
    11041 ggtggcctga ggtgccgatc gagtcgcagg tcgcgcgcgg cgccgcgttc cttacgctgt
    11101 tgcgcgagcg cttggtgatc ctcgacggcg ccatgggaac gatgattcaa cggcacgccc
    11161 tgaccgaggc gcagttccgc ggcgagcgct tcgccgcctg ggcctgcgat ctgcgcggca
    11221 acaacgatct attgtcgatc agccagccgg acatcatcgc cgcaattcac cgcgactact
    11281 tgctcgcggg cgccgatgtc atttccacga acacattcaa ttccaccgcc atctcgcagg
    11341 cggactacgc gatgcaatcc ctcgcggtcg aactcaacac gacggcggcg cggctcgcgc
    11401 gccgggtggc ggatgagatc agcgcagcca cggggcaaca gcgctttgtc gcgggagcgc
    11461 tcggcccgac cagccgcacg gcctcgctgt cccccgacgt caacgatccc gggtttcgca
    11521 atgtgagttt cgatgagttg gtaagcgctt actcggcggc gacccgcgcg ctcatcgatg
    11581 gcggcacgga tatcattctc atcgagaccg tgttcgatac cttgaacgcc aaggcggcgc
    11641 tctacgccgt gcgcacggtg ctcgatgagg ccggcttgga cttgcccctg atcgtgtcgg
    11701 gcacgatcag cgatgcctcg gggcgcacct tgtcgggcca gacgaccgag gcgttttgga
    11761 attcgattcg tcatgcacgc ccggccgtcg tcggcctcaa ctgtgcgctc ggcggcaggc
    11821 agctgcgtcc gtacatcgaa gagatggcac gcatcgccga tacctatgtg tgcgcctatc
    11881 ccaatgcggg cttgccgaat gccttcggtg aatacgacga aaccgcgcat gaaaccgccg
    11941 ccatcctcgc ggagttcgcg gccagcggct tcgtcaacat ggtcggcggg tgctgcggca
    12001 cgacgccgga gcacattcgc caaatggcgc gcgcggttgc aggcatcgcg ccgcgggcgc
    12061 tgccgagcat tgcgcccgcc tgccgtctcg ccggtctcga gccgctcacc atcgatgccg
    12121 acagcctgtt cgtgaacgtc ggcgagcgca ccaacgtcac cggatcggcg aagtttcgca
    12181 agctcatcga ggccaacgat tatccggcgg cggtggacat cgcgcggcag caagtcgcca
    12241 acggcgcaca gatcatcgat gtgaacatgg acgaaggcat gctcgattcg caagcggcca
    12301 tggcgaaata cttgagcctg atcgccggcg agccggatat cgcccgcgtc cccgtcatga
    12361 tcgattcgtc caagtggtcg gtgatcgagg cgggactgaa gtgcgtgcaa ggcaagccga
    12421 tcgtcaattc catcagtttg aaggaaggcg aagaggcgtt tctcgcgcac gcgcgccagt
    12481 gcatgcgcta tggcgcggcg gtggtggtca tggccttcga cgagcgcggc caggcggata
    12541 ccatcgatcg aaaagtggcg atctgcgagc gcgcctaccg gctgctcacc gagcgcctcg
    12601 gcttcatggc ggcggacatc atcttcgatc cgaacatttt tgcggtggcg accggcatcg
    12661 aggaacacaa tcgctacagt ttggatttca tcgaggcgac cgcggcgatc aagcagcgca
    12721 tgccgggggt gcacgtcagc ggcggcgtga gcaatgtgtc gttctcgttt cgcggcaacg
    12781 acccggtgcg cgaagcgatg cactcggtgt ttctgtatca cgcgattcgc gccggcctca
    12841 ccatgggcat cgtcaatgcc gggcaactgg ccatctacga agacataccg accgagctgc
    12901 gcgagcgctg cgaggacgtc atcttggcgc ggcgcgcgga tgccacggaa cggctgctcg
    12961 agattgcgca atccttcaag ggaggcggtg ctcaaaagcg cggcgacgat ctcgagtggc
    13021 gcaatctgcc cgtggcagag cgcctgcagc atgccttgat caaggggctg gacgaattcg
    13081 ttttgcagga caccgaggag gcgcggctgc aggcgaagcg gccgctcgac gtcatcgagg
    13141 gccccctgat ggaaggcatg aacgtggtcg gcgatctgtt cggtgccggc aagatgttcc
    13201 tgccgcaggt ggtcaagtcg gcgcgcgtca tgaaaaaagc ggtatccgtg ctggtgccgt
    13261 acatcgagaa ggaaaaggac ggcagcagcc gttcaaacgg caaggtggtc atggccaccg
    13321 tcaagggcga cgtgcacgac atcggcaaga acatcgtcgg cgtggtgttg cagtgtaata
    13381 atttcgaggt gctcgatctg ggcgtgatgg tctcctgcga gaaaatcctc gagaccgcaa
    13441 cgcgcgaggg cgcgagcctg atcggcttgt ccggattgat cacgccctcg ctcgatgaaa
    13501 tggtgcatgt tgcgaaggag atgcagcgcc tgggctaccg gcagccgctg ctcatcggcg
    13561 gcgccacgac ctcgcccgcg cacacggcgg tgaagatcga tccgcagtac cagggcgcgg
    13621 tggtgtacgt gaaggatgcc tcgcgctcgg tcggtgtgtg ccagcaattg gtgaccgagg
    13681 gcacgcgcga tgcgtacatc gccacactca aggcggaaca cgcgaggcgc cgcgagcagc
    13741 atcgcaacaa gggcgtgaaa gcgccgcggc tttcgctgct gcaagcgcgg gcgaagcgtc
    13801 tgcatatcga ttgggccggc tatgtgccgc ccgtgccgag tttcctgggc gtgcgcgcct
    13861 tcgatgac
//
LOCUS       DASWZT010000211         2696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_59616, whole genome shotgun sequence.
ACCESSION   DASWZT010000211 DASWZT010000000
VERSION     DASWZT010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59616"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VGE92_05765"
     CDS             <1..381
                     /locus_tag="VGE92_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313358.1"
                     /translation="IVFSTNLEPAELADDAVLRRLGYKIELGPLSVDLYTRVWVSVCD
                     EMKVEFDLRLLSYVLNKLYAASGRPLLACHPRDLICMALDKAAYDERGNDFNAEDLRW
                     AWSNYFLPGEESGEAAKRGSLGSR"
     gene            389..1162
                     /gene="cpaB"
                     /locus_tag="VGE92_05770"
     CDS             389..1162
                     /gene="cpaB"
                     /locus_tag="VGE92_05770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEY1313359.1"
                     /translation="MLIATSLLLAGSAAWMANRWLAARAAPAPVVNVHILASAMNLPV
                     GTKIDSKQVKVMEVLPGEEPAGAFHDLKEIEGKVTVANVSAGQLLLAPMFIKEGDSAL
                     AAVVGRDKRAVTVRVDDVVGVAGFLMPGNRVDVVATHKDDKTGAMTSETILSDIKVLA
                     VDQTTAVNSNEPVVVHAVTLEVTPSDGETLLMGKAEGSIQLALRNPLDKGDARHKPPP
                     PKIVAKAPVVVRDPAIMVIRGTLVGRDSQNNNSAQNNKQ"
     gene            1269..>2696
                     /locus_tag="VGE92_05775"
     CDS             1269..>2696
                     /locus_tag="VGE92_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HEY1313360.1"
                     /translation="MNAYAQTQQLKPPPIRAVAQPPLLAGGASIDLAVPLYQSRVVTV
                     VNPAQRVSVANPDVADIVVISPTELYVLGKDLGTTNVLLWDRDNHLIGAIMVEVQHDI
                     DSLRRKLAELLPGQPIEARASQRSIVLSGRVSDAEAMNAAVRIASTFLAQVQTAKQTE
                     QFQQGNGSRREDKTVGDVINLLQIGGAQQVMLEVKVAEIARTEVMSLDLQFNSIARGL
                     GNWNVGGVNGGATFPTGFLQPPNSLLPSPYNTLPLFTPNAGTVTSALSNNGVVSPFGA
                     ATTQFLPSTQTITNQGLFATFLDKNFLFNMALDASKQKGLARILAEPTLTTLSGQEAT
                     FLSGGEFPIPVPQSLGQVTIQFKQYGVSVKFLPVVLNSGHINLSLNLTVSELASNNTV
                     TVAVPGSSASYTIPSLTERSAAGTVELGDGQTIGLAGLLNSTTASQVTKFPGLGSIPI
                     LGALFRSQNYQKGETELVILVTPRLA"
BASE COUNT          549 a          763 c          846 g          538 t
ORIGIN      
        1 atcgtgttct cgacgaattt ggaacccgcc gagctcgcgg atgacgcggt gctgcggcgc
       61 ctcggctaca aaatcgagct cgggcccttg agtgttgatt tgtatacccg ggtatgggtg
      121 tcggtctgtg acgagatgaa agtcgagttc gatcttaggc tcttaagcta cgtattgaac
      181 aagctgtatg cggcatcggg ccggccgctg ctcgcctgcc atccgcgcga cctgatctgc
      241 atggcgctcg ataaagccgc ctatgacgag cgcggcaacg atttcaacgc cgaggatttg
      301 cgctgggcgt ggtcgaatta ttttctccca ggtgaagaga gcggcgaggc cgcgaagagg
      361 ggtagtcttg gctcgaggta gaggacctgt gctgattgcg acgtcgctgc tgcttgcagg
      421 cagcgccgcg tggatggcga accgatggct ggcggcgcgc gcggcgcctg cacccgtggt
      481 gaatgtgcac atcctcgcgt cggcgatgaa tctgcccgtg ggaaccaaaa tcgatagtaa
      541 gcaggtgaag gtcatggaag tcctgccggg tgaggaaccc gccggcgcat ttcacgactt
      601 aaaggaaatc gagggcaaag tgacggtggc gaacgtgtcg gcggggcaac tgttgctcgc
      661 ccccatgttc atcaaggaag gcgacagtgc gctcgctgcg gtggtcgggc gcgacaaacg
      721 cgccgtgacc gtgcgggtcg atgatgtggt cggcgtggcc ggctttttga tgccgggcaa
      781 tcgagtcgat gtggtcgcga cccataagga cgacaagacc ggcgccatga cttcggaaac
      841 catcttgagc gacatcaagg tgctggcggt cgatcagacg acggcggtga attcgaacga
      901 accggtcgtg gtgcacgccg tgacgctcga ggtgacgccc tcggacggcg agacgctgct
      961 catgggaaaa gccgaaggtt cgattcagct ggcgctgcgt aatccgctcg ataaaggtga
     1021 cgcgcggcac aaaccgccgc cgccgaaaat cgtggccaag gcgccggtgg ttgtgaggga
     1081 tccggcgatt atggttatcc gcgggacatt agtgggtcgt gactcgcaaa acaataacag
     1141 cgcgcagaac aataaacagt gacgaggtga atgtgccagc cgggagtagg gatgagtcga
     1201 ttggaagcag caggttcttt cgagtaggca ccgttgcagt ggtttcgacc gtgtggagct
     1261 tgacttacgt gaatgcgtac gcccagacac agcagctcaa gccgcccccg atcagggcag
     1321 tggcgcagcc gccgctgctg gcgggcggcg cgagcatcga tttggcggtg ccgctgtatc
     1381 agtctcgcgt ggtgacggtc gtgaatccgg cgcagcgcgt ctcggtcgcg aatccagacg
     1441 tggctgacat cgtggtcatt tccccgaccg aactctatgt gctgggcaag gaccttggca
     1501 ccacgaatgt gctcctctgg gaccgcgata accatctcat tggcgccatc atggtcgaag
     1561 tacaacacga catcgacagt ctgcgacgca aattggccga actgttgccc ggccagccca
     1621 tcgaagcgcg cgcctcgcag cgttcgatcg tgctgtcagg ccgcgtcagc gatgccgaag
     1681 ccatgaatgc cgcggtgcgc attgcttcga ctttcctagc gcaagtgcag accgcaaagc
     1741 agaccgagca gtttcagcaa ggaaatggct cgcgccgcga agacaaaacg gtcggcgatg
     1801 tcatcaactt gctgcagatc ggcggcgcac aacaagtcat gctcgaggtc aaagtcgctg
     1861 aaattgcgcg caccgaagtg atgtcgctgg atttacagtt caacagtatt gcgcgcgggc
     1921 tcggcaactg gaatgtcggc ggcgtcaacg gcggcgcgac cttcccgacg ggattcttgc
     1981 aaccgccgaa cagccttttg ccttcaccgt acaacacttt gccgcttttt accccgaatg
     2041 ccggtactgt aacttccgcg ctgtcgaata acggtgtcgt gtcacccttt ggcgcggcga
     2101 ccacgcaatt cctcccctcg acgcagacaa ttaccaatca agggctgttt gccacttttc
     2161 tggacaagaa cttcctgttc aatatggcgc tcgacgcctc gaaacaaaag ggtctcgcac
     2221 gtattctcgc tgaacccacg ctcaccacgt taagcggcca agaagcgacc tttctctccg
     2281 gtggagaatt tcccattccg gtgccgcaaa gtttggggca ggtcaccatc cagttcaaac
     2341 aatacggcgt gtcggtcaag tttctacccg tggtgctcaa cagcggtcat atcaacttgt
     2401 cgctgaatct caccgtgagc gagttggcga gcaataatac ggtgacggtg gcggtaccgg
     2461 gctcgtcggc cagttatacg attccttcgc tgaccgagcg cagcgccgct ggcacggtgg
     2521 aactcggcga cggccagacc atcggtctcg ccggtttgtt gaattcgacc acggcgagtc
     2581 aggtgaccaa gtttccgggt ctcgggtcga ttcccatact gggtgcgctg ttccgcagcc
     2641 agaactatca gaagggcgag acggagctcg tcattctcgt caccccgcgg ctcgcg
//
LOCUS       DASWZT010000212        10124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_6321, whole genome shotgun sequence.
ACCESSION   DASWZT010000212 DASWZT010000000
VERSION     DASWZT010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10124
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6321"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /locus_tag="VGE92_05780"
     CDS             complement(<1..1350)
                     /locus_tag="VGE92_05780"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEY1313361.1"
                     /translation="MARSPSIAHNADVRFLGKLLGDVIRTYEGEPLFRRIEAIRAASV
                     DRYRGIANPRGLAVGLDTLSLDETVAFVRSFMLFSMLANLAEDRQGVAAEEGSTLASA
                     LEFLSGQGIARDAAAALLERSLITPVLTAHPTEVMRKSMLDHRNRIAALLWMRDMDYS
                     ETPQGELIEEGIAAQIALLWQTRALRRERLYVADEVDTALTYLKDIFLPVVPLLYARW
                     ERLLGRRPASFLRLGSWIGGDRDGNPHVTAESLRLALGRASQVLLVEYLEQLNALGAE
                     LSVSSELAGVSAALDELAQRSGDINAARTDEPYRRAITGIYARLAATYQHLTGRAPPR
                     PASVSAPPYADPEDLLTDLKIVEQSLKSESNVGYHAGGSLSRLARAVETFGFHLATLD
                     LRQNADVHARVVAELLHSAGVVPDYHALDEAERVRLLRRELAGERLLASPYSSYSEET
                     "
     gene            1468..1995
                     /locus_tag="VGE92_05785"
     CDS             1468..1995
                     /locus_tag="VGE92_05785"
                     /inference="COORDINATES: protein motif:HMM:NF004638.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEY1313362.1"
                     /translation="MSDKLPRRVIPIKVEPPSSLSTAERSAVLSACARLKSMPGALLP
                     VLHAVQEALTYVPKDAVPLIAQELNLSIAEVHGVVSFYHYFLQKRPGRHVLHLCRAEA
                     CQALGSVALEAHVKKSLGVDYHGTTTDGAVTLEPVFCLGNCALGPSLMIDQQLHGRVS
                     AERFDALLAQSREST"
     gene            1992..3545
                     /locus_tag="VGE92_05790"
     CDS             1992..3545
                     /locus_tag="VGE92_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEY1313363.1"
                     /translation="MKIYVPRDASALAMGADRTAAAIAAEAKRRGIEIELIRNGSRGL
                     LWLEPLVEVEVAGTRIAYGPLQADAVAALFDAGFTEGKAHALGHGATEEIPYFKQQNR
                     LTFARVGLTDPRSLKDYVAHGGYRGLAAALGAAPAEIVAKVSASGLRGRGGAAFPAGI
                     KWKTVLGAQNAQKYVVCNADEGDSGTFSDRMLMEGDPFVLIEGMTIAGLATGADRGFI
                     YLRWEYPQAKLALEAAIAAARAENYLGQDIRGSGKAFELEVRMGAGAYICGEETSLLE
                     SLEGKRGQVRFKPPLPALEGLFGKPTVINNVITFASVPIILDRGAEFYQHFGMGRSRG
                     TLPMQLAGNIKFGGLVEIAFGMTLRELLYDYGGGSYSGRPLRAVQVGGPLGAYLPESQ
                     FDTPLDYEAFAAIKGMLGHGGIVAFDDSVDMAAQARYAMEFCAIESCGKCTPCRIGST
                     RGVEVIDRLVAGENAAKQETILRDLCETLTYGSLCALGGLTPYPVISALNHFPQDFVK
                     PVGAASASG"
     gene            3566..6421
                     /gene="fdhF"
                     /locus_tag="VGE92_05795"
     CDS             3566..6421
                     /gene="fdhF"
                     /locus_tag="VGE92_05795"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEY1313364.1"
                     /translation="MLSIAETDLGTPARTSAELVTLTIDGQEVTVPAGTSVMRAAALC
                     EVKIPKLCATEQLEAFGSCRLCLVQIEGMKGLPASCTTPVAPGMKVTTQNKQLADVRR
                     GVMELYISDHPLDCLTCPANGHCELQDMAGAVGLREVRYGYEGENHLSAKKDESNPYF
                     TFDASKCIVCSRCVRACDEQQGTLALTIQNRGFDSSVAASQNVSFMDSECVSCGACVQ
                     ACPTATLSEKSLIDQGQAEHSLITTCAYCGVGCSFRAEMKGEEVVRMVPNKDGHANHG
                     HSCVKGRFAIGYATHADRIVKPMIRSKISDPWREVSWEEAIGYAASELKRIQAQYGRN
                     SIGGITSSRCSNEETYLVQKLVRAGFGNNNVDTCARVCHSPTGYGLKNTLGESAGTQN
                     FDSVMKADVIFVIGANPTDGHPVFASQMKRRLRQGAKLIVADPRRIDLVRTPHITAEQ
                     HLKLRPGTNVALINSLAHVIVTEGLVKEDFVRERCEIPSFEKWRHFVAQPVNSPEAMQ
                     EVTGVAAADVRAAARLYATGGNGAIYYGLGVTEHSQGSTMVMGIANLAMATGNIGREG
                     VGVNPLRGQNNVQGSCDMGSFPHEFPGYRHVSDDTVRHLFEGAWGVPLQNEPGLRIPN
                     MFEAALDGEFKGLYIEGEDIAQSDPNTQHVTAALTSMECIIVQDLFLNESAKFAHVFL
                     PGSSFLEKDGTFTNAERRISRVRKVMQPKSGYADWEITQLLANAVGYPMNYSHPSQIM
                     DEIARLTPTFTGVSFARIDELGSIQWPCNAEHPEGTPTMHIGQFVRGKGKFLVTEYVP
                     TAEKVNARYPLILTTGRILSQYNVGAQTRRTENNRWHDEDRLEIHPHDAEERGIAEGD
                     WVGIASRAGDTVLRARVTERVQPGVVYTTFHFPESGANVITTDNSDWATNCPEYKVTA
                     VQATRVSQPSEWQKRFREFTEEQETLLKGADVMFVAK"
     gene            6418..6669
                     /locus_tag="VGE92_05800"
     CDS             6418..6669
                     /locus_tag="VGE92_05800"
                     /inference="COORDINATES: protein motif:HMM:NF022832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HEY1313365.1"
                     /translation="MTHEGAEHLVKMANDIGNFFRAEPEREDAIAGIANHISKYWTKR
                     MREKLAAHLREEGDTALDELPRAALKRLNPLAAPAEAPR"
     gene            6787..7818
                     /locus_tag="VGE92_05805"
     CDS             6787..7818
                     /locus_tag="VGE92_05805"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HEY1313366.1"
                     /translation="MIVKPWTRCLLAALMLVSLPVLSQAQVTISVNVAPPELPVYEQP
                     PIPEDGYVWTPGYWAWGDDIQDYYWVPGTWVAAPQPSYLWTPGYWGWSGGVYLWNAGY
                     WGPHVGFYGGVNYGYGYGGNGYEGGYWQNGHLYYNRSVTNIGTTNITNVYNKTVINNV
                     TVNRVSYNGGSGGVQAHATPAELTAAHEQHVAAVPAQRQQEQTARGNSSLRASANHGN
                     PPIAATARAGVFSGAGVVAAKHGSGMSVVHANPPPQAHPAAESHAPAPPPPHAPRPPA
                     EAPRQQAPAQHPPEARPEARPEARPPQAREPERVEAPHPNAAPPRPRPEAHEPHEQHP
                     PAQEDHDHH"
     gene            7805..8152
                     /locus_tag="VGE92_05810"
     CDS             7805..8152
                     /locus_tag="VGE92_05810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1313367.1"
                     /translation="MTTISHDSVAHRFTTEVDGSHAELEYSLSGDVMSITHTRVPPAI
                     GGRGVAAELMGAALAAARTAGWSVQPICSYAVAYMARHPEHSAKQHMDELLDEALDES
                     FPASDSPAVGGSN"
     gene            complement(8149..8721)
                     /locus_tag="VGE92_05815"
     CDS             complement(8149..8721)
                     /locus_tag="VGE92_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY1313368.1"
                     /translation="MAKQSNKSQPFLTDVKTLRERARKNISSGGVTFTYEGDVKKTIA
                     ILQAVLATEIVCVLRYTMHAVAATGISSEGVKAEFAQHAKEEAGHMQEVAERINQLGG
                     IPDFNPTGLATRAASEYGTAGNLIEMIKENLIAERIAVDHYRELIRYFGNNDPGTRSM
                     LEGILTVEEEHANDMHDLLVAHEGRPMLPG"
     gene            complement(8826..9638)
                     /locus_tag="VGE92_05820"
     CDS             complement(8826..9638)
                     /locus_tag="VGE92_05820"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY1313369.1"
                     /translation="MKAKVVAILETRTGAHLGELIARRGGIPLLAPALEEVPDVDPHA
                     VSALLAQWREAPFKMAIFQTGVGTRALFQATDAAGLSGELLARLGECVVVVRGPKPVG
                     ELTARSVRIDLRAATPFTTEMVLAAVADTPVQGERVMVQRYGAANRDLRAALEARGAR
                     VEEIATYRWALPADTRPLIDLLDALARSAVDAVVFTSAVQIHNLYAVAEQAGRAPQLA
                     GQLQRVIVASVGPVCSKALREHGVEPSFEADPPKLGPLVAGLDAALSANHRH"
     gene            9718..>10124
                     /locus_tag="VGE92_05825"
     CDS             9718..>10124
                     /locus_tag="VGE92_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313370.1"
                     /translation="MPPRSTLLLAVILALLCGHPAPAADADSVRQAFVAAMQRVRQNL
                     PDTPDSPELDSYVIHDYLLAARMRRDLAKTPDDALDAPIDAFLQAHANQPVTHALKHD
                     WLLSLAARHRWDWFLPRSADVTDAQLLCDRLAGR"
BASE COUNT         1891 a         3297 c         3179 g         1757 t
ORIGIN      
        1 ggtctcctcc gagtagctcg aatagggact cgccagcaga cgttcacccg cgagttcgcg
       61 ccgcaatagg cgcacgcgtt cggcttcgtc gagcgcgtgg taatccggca cgacgccggc
      121 actgtgcaac agttcagcca ccacgcgcgc gtgcacatcg gcgttctgac gcaggtcgag
      181 cgtcgcgagg tgaaagccga aggtctccac cgcccgcgcc aagcgagaca gcgagccgcc
      241 ggcgtggtac ccaacgttgc tctcggactt tagcgactgc tcgacgatct tcaagtccgt
      301 cagcaggtcc tcggggtcgg cgtacggcgg ggcgctcacc gacgccggcc gcggcggcgc
      361 tcgtccggtt aaatgctggt aggtggcggc gagccgcgca tagataccgg tgatggcgcg
      421 ccggtagggt tcatcggtgc gcgccgcgtt gatatcgccg ctgcgttgcg cgagttcatc
      481 gagggcggcg gatacgcccg cgagttcgct cgacaccgac agttccgcac cgagcgcatt
      541 cagctgctcc aagtactcca ccagcaacac ctgcgaggcg cgacccaacg ccaagcgcag
      601 cgactcggcc gtgacgtgcg gattgccgtc acgatcgccg ccgatccagc tgccgaggcg
      661 caggaaactg gcggggcgcc ggccgagcaa gcgctcccag cgcgcataca acagcggcac
      721 gacggggaga aatatatcct tgagatacgt gagcgcggta tcgacttcgt cggccacata
      781 cagccgttcg cggcgcaacg cgcgcgtttg ccagagcagc gcaatctgtg cggcgatgcc
      841 ctcttcgatc aactctcctt gcggcgtttc gctgtagtcc atatcacgca tccacaagag
      901 tgcggcgatg cggttgcggt gatcgagcat gctcttgcgc atgacttcgg tgggatgcgc
      961 ggtcagcacc ggcgtaatga gggagcgctc cagcaacgcc gcggcggcgt ctcgcgcaat
     1021 gccctgaccg ctcaggaact cgagcgccga tgccagggtg ctgccctcct cggcagccac
     1081 gccctgccgg tcctctgcga ggttggcgag catcgagaaa agcatgaagc tgcgcacgaa
     1141 ggccacggtc tcatccagcg ataaggtgtc gaggcccacg gccaagccgc gcggattggc
     1201 gattccgcga taacgatcca cggacgctgc gcgaatcgcc tcgatgcggc gaaacaacgg
     1261 ctctccctcg taggtgcgga ttacgtcacc taaaagcttg cctaaaaagc gaacgtctgc
     1321 gttgtgggcg atggacggcg atcttgccat ggcggacaat attgcacgtg gcgcggctgt
     1381 acgcctgacc tagcgtgccc ttctctccgg tggaatacac ggttgataag gcgtaatatc
     1441 tgatatatct atgggcaaca tcgtagcatg agtgataaat tgccacggcg ggtgatcccg
     1501 atcaaagtcg agccgccctc gagcttgagt acggcggagc gctcggcggt gctctctgcc
     1561 tgtgcgcgct tgaaatcgat gccgggggcg ctcctgcccg tcctgcacgc ggtgcaggaa
     1621 gccctcacct acgtgcccaa ggatgccgtt cccctgattg cccaggaact gaacctttcc
     1681 atcgcagaag tccatggcgt ggtcagtttc taccattact ttctgcaaaa acgtcccggc
     1741 cggcatgtgc tgcacctgtg ccgggccgaa gcctgtcagg cgctcggcag cgtggcgctc
     1801 gaggcgcacg tgaaaaagag cctcggggtg gactatcacg gcaccacgac cgatggcgcg
     1861 gtgaccttgg aaccggtgtt ttgcctgggt aactgcgcct tggggccgtc tctgatgatc
     1921 gaccagcaat tgcacggccg cgtgagcgcg gagcgatttg atgcgttgct ggcgcagagc
     1981 cgggagagca cgtgaaaatc tacgtgccgc gcgatgcctc ggcgctcgcc atgggggccg
     2041 atcgcaccgc cgcggcgatc gccgcagaag ccaagcggcg cgggatcgaa atcgaactca
     2101 ttcgcaatgg ctcgcgtggg ctgctctggc tcgaaccctt ggttgaagtt gaggtcgcgg
     2161 ggacccgcat cgcctacgga ccgctgcaag ccgacgctgt ggcggctctg ttcgacgccg
     2221 gattcaccga gggtaaggcg cacgccctgg ggcacggtgc gaccgaggaa atcccctact
     2281 tcaagcaaca gaatcgcctc accttcgccc gggtcggtct caccgatcca cgcagtctca
     2341 aggattacgt ggcgcacggc ggctatcgcg gcctcgcggc agccctgggc gccgcgccgg
     2401 ccgagatcgt cgccaaggtc agcgcgtccg gacttcgcgg ccgcggcggc gccgcatttc
     2461 cggccggcat caagtggaag acggtgttgg gggcgcagaa cgcgcaaaag tacgtcgtct
     2521 gcaatgccga cgaaggcgac tccggaacat tctcggatcg tatgctgatg gagggcgatc
     2581 cgttcgtgct gatcgagggc atgaccatcg ccggcttggc cacgggcgcc gaccgcggtt
     2641 tcatctactt acgctgggaa tatccgcagg caaagctcgc gctcgaggcc gctattgccg
     2701 cggcacgggc ggagaactac ctgggccaag acatccgcgg cagcggcaaa gcctttgagc
     2761 tggaagtgcg catgggtgcc ggtgcctata tttgcggcga ggagacatcg ctcctcgagt
     2821 ctttggaggg caagcgcggt caagtacgtt tcaagccgcc gctgcccgcg ctagagggcc
     2881 tgttcggcaa gcccacggtg atcaacaacg tcattacctt cgcttcggtg cccatcattc
     2941 tcgaccgcgg tgcggagttc tatcagcact tcggcatggg ccgctcgcgc ggcacgctgc
     3001 cgatgcagct tgccggcaac atcaagttcg gcggcctggt ggaaatcgcc ttcggcatga
     3061 ccttgcgcga gctgctctac gactacggcg gcggcagtta cagcggccgg ccgctgcgcg
     3121 cggtacaggt cggcggcccc ttgggcgcct atctgccgga gtcgcaattc gacacgccct
     3181 tggactatga ggccttcgcc gccatcaagg gcatgctggg gcacggcggc atcgtggcct
     3241 tcgacgacag cgtcgatatg gcggcgcagg cgcgctacgc catggagttc tgcgccatcg
     3301 agtcctgcgg caagtgcact ccgtgccgaa tcggttcgac tcgcggggtt gaagtcatcg
     3361 atcgcctcgt tgccggcgag aacgccgcca aacaggagac gattttgcgc gatctgtgcg
     3421 agactttgac ctacgggtcg ttatgcgcac tcgggggctt gaccccgtat ccggtcattt
     3481 ctgccttgaa ccatttcccc caagatttcg tcaaacccgt aggggcggcc agcgcctccg
     3541 gttgaaggta cacgaggatc cacctatgtt gtccattgct gaaacagatc taggcacccc
     3601 ggcgcgaacc agcgccgaac tcgtgaccct caccatcgac ggccaggaag tgactgtgcc
     3661 cgccggaaca tcggtgatgc gcgccgccgc gctgtgcgag gtgaagatcc ccaaactgtg
     3721 tgccaccgaa cagctcgagg ccttcggctc ctgtcgtctg tgcctggtgc agatcgaagg
     3781 catgaagggg ttgccggcct cctgcaccac gccggtcgcg cctggcatga aagtcacgac
     3841 gcaaaacaag cagctcgccg acgtgcgccg cggcgtcatg gagctgtaca tctccgatca
     3901 cccgctggat tgcctgacct gtcccgccaa cggccactgc gaactgcaag acatggcggg
     3961 cgccgtcggc ctgcgcgaag tgcgctatgg ctacgagggc gaaaaccatt tatccgcgaa
     4021 gaaggacgaa agcaatcctt acttcacctt cgatgccagc aagtgcatcg tgtgttcgcg
     4081 ctgcgtgcgc gcctgcgacg aacagcaagg caccttggcg ctgaccatcc agaatcgcgg
     4141 cttcgattcg agcgttgcgg cgagccagaa cgtctcgttc atggattccg aatgcgtgtc
     4201 ctgcggcgcc tgcgtccagg cctgtcccac cgccacgctg tccgagaaat cgctgatcga
     4261 ccagggtcaa gccgagcaca gcctgatcac gacctgcgcc tactgcggcg tcggctgctc
     4321 gttccgcgcc gaaatgaaag gcgaggaagt cgtgcgcatg gtgccgaaca aggacggtca
     4381 cgccaatcac ggccactcct gcgtgaaggg acgcttcgcc atcggttatg ccacgcacgc
     4441 cgatcgcatc gtcaagccca tgatccgctc caagatctcc gacccctggc gcgaggtgtc
     4501 gtgggaagaa gcgatcggct acgcggcctc cgagctcaag cgcattcaag cccagtacgg
     4561 ccgcaactcc atcggcggca tcacctcctc gcgctgctcg aatgaggaga cctacctggt
     4621 gcagaaactg gtccgcgccg gtttcggcaa caacaatgtc gacacctgcg cacgcgtctg
     4681 ccattcgccc acgggctatg gtttgaagaa cacgctgggt gaatcggcgg gcacccaaaa
     4741 cttcgactcg gtgatgaagg cggatgtgat tttcgtgatc ggcgcaaatc ccacggatgg
     4801 tcatccggtg ttcgcttcgc aaatgaagcg ccgtctgcgc caaggggcaa agctcatcgt
     4861 cgccgatccg cggcgcattg acctcgtgcg cacgccgcac atcactgccg aacagcactt
     4921 gaagctgcgc cccggcacca acgtcgcgct gatcaattcc ctggcccacg tcatcgtcac
     4981 cgaagggctg gtcaaagagg atttcgtgcg cgagcgctgc gaaatcccgt ctttcgagaa
     5041 atggcgccac ttcgtcgcgc agcccgtgaa ttccccggaa gccatgcagg aggtcacggg
     5101 agttgcggcc gccgacgtgc gcgccgcggc gcgcctctac gccaccggcg gcaacggcgc
     5161 catttactac ggcctcggcg tcaccgaaca cagccagggt tccaccatgg tgatgggcat
     5221 cgcgaacctc gcgatggcca ccggcaacat cggccgcgaa ggcgtcggtg tgaacccgct
     5281 gcgcgggcaa aacaatgtgc agggctcctg cgacatgggt tcgttcccgc atgagttccc
     5341 cggctaccgc cacgtatcgg acgacacggt ccgtcacctg ttcgaaggcg cctggggtgt
     5401 cccgctgcaa aacgaaccgg gcctgcgcat tccgaatatg ttcgaggcgg cgctcgacgg
     5461 agaattcaag gggctgtata tcgagggcga ggacatcgcg cagtcggatc cgaatacgca
     5521 gcatgtcacc gcggcattga cctcgatgga atgcatcatc gtgcaggact tgttcttgaa
     5581 cgagtcggcg aaattcgcgc acgtgttcct gccgggatcg tcgttcttgg aaaaggacgg
     5641 cactttcacc aacgctgaac ggcgcatctc gcgcgtacgc aaggtaatgc agccgaaatc
     5701 aggctatgcg gactgggaga taacgcaatt gttggcgaat gccgtcggct acccgatgaa
     5761 ctattcgcat ccgtcgcaga tcatggatga gatcgcgcgg ctcaccccga cctttaccgg
     5821 tgtgagcttc gcccgcatcg atgagctcgg cagcatccaa tggccgtgca acgcggagca
     5881 ccccgagggc acgccgacca tgcacatcgg ccaattcgtg cgcggcaagg gcaagttcct
     5941 cgtcaccgaa tatgtgccca cggccgagaa ggtcaacgca cgctatccgt tgatcctgac
     6001 caccggacga atcttgagcc agtacaatgt cggcgcgcaa acgcggcgca ccgaaaacaa
     6061 ccgatggcac gatgaagatc gcctggagat ccatccgcac gatgccgagg agcgcggcat
     6121 cgccgagggc gactgggtgg gcatcgccag ccgcgcgggc gacacggtgc tgcgcgcccg
     6181 ggtcaccgag cgggtgcagc cgggcgtggt ctacaccacc ttccatttcc cggaatcggg
     6241 cgccaacgtg atcaccaccg ataattcgga ctgggcgacc aactgcccgg agtacaaggt
     6301 gaccgcggtg caagccaccc gtgtgagcca accttccgaa tggcagaagc gcttccgcga
     6361 gttcaccgag gagcaggaaa cgctgctcaa gggtgccgat gtcatgttcg tcgccaaatg
     6421 acccacgagg gagcggagca tctggtgaag atggcgaacg acatcggcaa tttctttcgc
     6481 gccgagcccg agcgcgagga cgcgatcgcc gggatcgcca atcacatctc caagtactgg
     6541 accaagcgca tgcgcgagaa gctcgccgcg catctgcgcg aggagggtga cacggccctc
     6601 gatgagctgc cgcgcgcggc gctcaagcgg ttgaatccgc tcgcggcccc ggctgaggcg
     6661 ccccgctagc cgtaggaccg gcgtgagcgt tatcctacag cgggcggcgg gtagccaccg
     6721 atgagggccg gctgcgacac cctctacact ggcggtatgg tccgtacagc tacccggaga
     6781 taaagagtga tagtaaaacc ctggacccgg tgcctgttgg cggcgctgat gcttgtatct
     6841 ctgcccgtgc tgtcccaggc ccaggtgacc atttcggtca acgtcgctcc accggagctt
     6901 ccggtgtacg agcagccgcc gattcctgag gacggctacg tctggacgcc cggctattgg
     6961 gcctggggcg acgatattca ggactactac tgggttcccg gaacgtgggt cgcggcgccg
     7021 caaccgagct atctgtggac gcccgggtat tggggatggt cggggggcgt atacctctgg
     7081 aacgccggtt actggggacc gcacgtcggc ttctacggcg gcgtcaatta cggctatggc
     7141 tacggcggca acggctacga gggcggttat tggcagaacg ggcacctcta ctacaaccga
     7201 tccgtcacca atatcggtac cacgaatatc accaacgtct acaacaagac cgtgatcaat
     7261 aacgtcaccg tcaatcgcgt gagctacaac ggcggctcgg gcggcgttca agcacatgcg
     7321 acaccggccg aactgacagc ggctcacgag cagcacgtgg ccgcggtgcc cgcacaacgg
     7381 cagcaggagc agactgcacg tggtaactcg tcgctgcgag ccagcgccaa tcacggcaat
     7441 ccaccgatcg cggccacggc gcgggccggc gtattctccg gagctggcgt ggtggcggca
     7501 aagcatggga gcggcatgag tgtcgtgcac gccaatccgc cgccgcaggc ccatccagcg
     7561 gccgagagcc atgcgcctgc accgccgccg ccccacgctc ctcgtccgcc ggccgaggcg
     7621 ccgcggcagc aggcacccgc acagcacccg cctgaagcgc gaccggaggc gcgaccggaa
     7681 gcccgcccgc cgcaagcgcg cgaacctgag cgggtcgagg cgccccaccc gaacgcggcc
     7741 ccgccccgtc cgcgacccga ggcgcatgaa ccgcacgaac agcacccgcc cgcacaagag
     7801 gatcatgacc accattagtc atgattcagt cgctcaccgt ttcacgaccg aggtggacgg
     7861 cagtcacgcg gaactggagt acagcttgag cggcgatgtg atgtccatca cccatacccg
     7921 cgtgccgccc gccatcggcg ggcggggcgt cgcggcggaa ctcatgggcg cggcgttggc
     7981 cgccgcgcgt accgcaggct ggtcagtaca gccgatctgt tcctatgctg ttgcctacat
     8041 ggcacgacat ccggagcaca gcgccaagca gcatatggac gagttattgg atgaggcgct
     8101 cgatgagtcc tttcccgcca gcgactcgcc cgccgtgggc ggcagcaact agcctggcag
     8161 catggggcgc ccttcgtgcg ccaccagcag atcgtgcatg tcgttcgcgt gctcctcttc
     8221 cacggtcagg atgccttcca gcatgctgcg agtgcccggg tcattgttgc cgaaataccg
     8281 aatgagttcg cgatagtgat cgacggcgat gcgctcggcg ataagatttt ccttgatcat
     8341 ctcgatcaaa ttgccggccg tcccatattc ggatgccgca cgcgtcgcca ggccggtcgg
     8401 attgaaatcc gggatcccac ccagctgatt gatgcgctcc gccacctcct gcatatgccc
     8461 ggcttcttct ttggcatgct gcgcgaattc agccttgaca ccttcgctcg agatgccggt
     8521 ggcagcgacc gcatgcatgg tgtagcgcag tacgcaaacg atttcggtcg caagcacggc
     8581 ctgcagaatg gcgatggttt tcttgacatc gccttcatag gtgaaggtca cgccgccgct
     8641 cgaaatattc ttgcgcgcgc gctcacgcag agttttcacg tcggtcagaa acggctggga
     8701 tttgttgctt tgcttggcca ctattgttct cctgaacggc actgcacgga atcgttattt
     8761 atacacggtc agccaggcgc cgatcataca acctgcgggc gccccgcgtc agccgggtac
     8821 gtgattcaat gtcggtgatt cgccgacaag gccgcgtcga gccccgcaac caagggcccc
     8881 aacttcggag gatcggcttc gaaagacggc tcgactccat gctcccggag tgccttcgaa
     8941 cacacgggtc ccaccgaagc gacgatcacg cgttgcaatt gcccggcgag ttgtggcgcg
     9001 cgaccggctt gttcggccac cgcatataaa ttatgaatct gcacggcgct cgtgaacaca
     9061 accgcatcga ccgccgagcg cgccagcgca tccaggaggt cgatgagcgg ccgggtatcc
     9121 gccggcaggg cccagcgata ggtggcgatc tcctcgacgc gtgcaccccg cgcctcgagc
     9181 gcagcacgca agtctcgatt ggccgcaccg tagcgctgaa ccatgacgcg ctcaccctgc
     9241 acgggcgtgt cggccaccgc ggccagcacc atttccgtgg taaacggcgt cgcggcgcgc
     9301 aggtcgatgc gcacgctgcg ggcagtcaat tctccgacgg gcttgggtcc cctgaccacg
     9361 accacgcact cgccgagccg cgcgagcagt tcgccgctca ggcccgcggc atcggtggct
     9421 tggaacaatg cccgtgtacc cacgccggtc tgaaaaatcg ccatcttgaa cggcgcttcg
     9481 cgccactgcg cgagcagcgc gctgaccgca tgcgggtcga cgtccggcac ttcttcgagc
     9541 gccggcgcga gcaagggtat gccgccgcgg cgcgcgatca attccccgag gtgcgcaccg
     9601 gtgcgagttt cgagaatggc gacgaccttg gctttcatgc tcggcatatt caaccacgcc
     9661 ggcgtaccga atgcagcgtg aatcgcgctg ctcgccgcta acgcctaaga tgccgcgatg
     9721 ccgcctcgtt cgacattgct gctcgctgtg attttggcgc tgctctgcgg ccatcccgcg
     9781 ccggcggccg acgccgacag cgtgcgccaa gcattcgtcg ccgccatgca gcgtgttcgg
     9841 cagaatctgc cggacacgcc ggattcgccc gagctcgaca gctacgtgat ccacgactat
     9901 ctgctggcag cgcgcatgcg ccgcgatctt gccaagaccc cggacgatgc gctcgacgcg
     9961 cccatcgacg cattcctgca agcgcatgcc aatcaaccgg tcactcacgc tttgaagcac
    10021 gactggttgc tgagcttggc ggcacggcac cgctgggatt ggtttctgcc gcgctcggcg
    10081 gatgtcaccg acgcgcaact cctgtgcgat cggctcgcgg gccg
//
LOCUS       DASWZT010000213         2173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_93578, whole genome shotgun sequence.
ACCESSION   DASWZT010000213 DASWZT010000000
VERSION     DASWZT010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93578"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VGE92_05830"
     CDS             <1..708
                     /locus_tag="VGE92_05830"
                     /inference="COORDINATES: protein motif:HMM:NF014820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEY1313371.1"
                     /translation="GVARAYLETHRATAEDFGLVAVKNRRHAGMNPLAHYRAPQTLGE
                     VMNSRMIVDPLRLTMCAPTTDGAAALVLCSSDYARRLTRKSVQLVTSHVAASPGIGQS
                     PVTPAATAAYEASGVGPNDFDLIELHDAAAPAELLQYAEIGLCEEGAAHHLLREGKTS
                     IGGETPVNTSGGLLSRGHPLGATGCAQVVELCQQLRGEAGRRQVEDARVGLAINGGGW
                     LDGTYAVAVSTIVKRLD"
     gene            729..1556
                     /locus_tag="VGE92_05835"
     CDS             729..1556
                     /locus_tag="VGE92_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY1313372.1"
                     /translation="MSTRIELETCYHERVADGVAMLTLNRPERGNAVVPEMARDMLAT
                     LATLEQDRTVRVLVLTGAGKQFCAGADLVEFQRYIAEQLPIDHEPYNARVLWPVTQRL
                     VSCRLPLIAAINGGATAGGLDLALACDIRIASSRAKMGETYIKLGLNPGNGGTYFLPR
                     LLGSGLAAELAFTGDLIDAPRALEMGLVNRVVEPEALLTEATALAARIAAYPRLALEA
                     TKQQLRQSWHLDLISSMNLSFWGVATLSHTEDLKEGIAAARDKRPPQFNASDKKGHS"
     gene            1573..>2173
                     /locus_tag="VGE92_05840"
     CDS             1573..>2173
                     /locus_tag="VGE92_05840"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY1313373.1"
                     /translation="MSLRNTYRGLRVLDLGTNIAAPFAAMMLGDMGADVIKIERPPNG
                     DDTRALPPFWGHEATVFLSVNRNKRSVLLDIRSPEGRAALLKLAESADVIIESFPPGL
                     AGKFKLRFADLRAHCPRVVLCSVSAFGDGPLGATMPGYDALVQAVSGLMSFTGHPHTP
                     PTRLAPSVLDLTTGMWALIGIMAALTRRAAGGGAEHVRPA"
BASE COUNT          368 a          703 c          724 g          378 t
ORIGIN      
        1 ggcgtggcgc gcgcctatct ggagacgcac cgagccactg cggaggattt tggcctggtc
       61 gcggtgaaaa atcgtcggca tgcgggcatg aatccgctgg cgcactaccg cgcaccgcag
      121 acgctcgggg aggtcatgaa cagccgtatg atcgtcgatc cgctgcgtct cacgatgtgc
      181 gccccgacga ccgatggggc ggcggctctg gtattgtgtt cgtcggacta tgcccggcgc
      241 ctgacgcgaa aatccgtgca attggtgact tcgcatgttg cagccagccc cggcatcgga
      301 cagagtccgg tcacgcctgc agcgacggcc gcctacgagg cgtcgggagt tggaccgaat
      361 gacttcgatc tcatcgaact gcacgacgcg gccgcgccgg cagaattgct tcaatacgcg
      421 gagatcggtt tgtgcgagga aggcgcagcc caccacctct tgcgcgaagg caaaaccagc
      481 atcggcggcg agacgccggt gaacaccagc ggcggcctgc tcagtcgtgg tcatccgctc
      541 ggcgcaacgg gttgcgccca agtggtggag ctctgccagc agctgcgcgg tgaggccggc
      601 cggcgccagg tcgaggatgc tcgtgtcggt ctcgcgatca atggcggcgg ctggctggac
      661 ggtacgtatg cggtggcagt gtcgacgatc gtcaagagat tggattgacc tcatcacgag
      721 tcgcgcccat gagcacccga attgaacttg aaacctgcta tcacgagcgc gtcgcggacg
      781 gcgtggcgat gctcacgctg aatcggcccg agcgaggcaa tgccgtggtg ccggagatgg
      841 cacgcgatat gctcgctacg ctcgcgacgc tggagcagga ccgcacggtg cgcgtgttgg
      901 tgttgacggg tgccggcaag cagttctgcg cgggtgccga tctggtcgag tttcagcgct
      961 acattgccga gcagctgccc atcgatcacg aaccgtacaa cgcgcgcgtg ttatggccgg
     1021 tgacgcaacg cttggtgagt tgccgcttac cgctcatcgc cgccatcaac ggcggcgcca
     1081 cggcaggcgg gctcgacctc gcgctcgcgt gcgatattcg tatcgcgtcg agccgggcga
     1141 aaatgggtga gacttacatc aaactcggac tcaaccctgg taacggcggc acctacttcc
     1201 tgccgcggct gctcggatcc ggtctcgccg ccgaactcgc tttcaccggc gatctcatcg
     1261 atgcgccgcg ggcgctcgaa atggggctgg tcaaccgcgt ggtcgagccg gaggcgttgt
     1321 tgacggaagc gactgcactg gcggcgcgga tcgcggctta tccgcgactc gcgctcgaag
     1381 cgaccaagca gcagctgcgc cagagctggc acctcgatct catcagctcc atgaacttaa
     1441 gcttctgggg cgtggctacc ttaagccaca ccgaggatct gaaggaaggc atcgctgcgg
     1501 cgcgcgacaa gcgcccgccg cagttcaacg catccgacaa gaagggccac tcatgacact
     1561 cgctcgctgc gcgtgagtct gcgcaacacc taccgcggct tgcgggtgct cgatttgggc
     1621 accaatatcg cggcgccctt cgccgcgatg atgctgggcg atatgggcgc agacgtcatc
     1681 aaaatcgagc gtccgccgaa cggcgacgac acgcgcgctc tgccgccctt ctgggggcac
     1741 gaggcgacgg tgttcctctc ggtcaatcgc aacaaacgca gcgtgctgct cgacattcgc
     1801 tcgccagaag ggcgcgccgc attgttgaag ctcgccgagt ccgccgacgt cattatcgag
     1861 agctttccgc ccggcttggc gggaaagttc aagcttcgct tcgcggacct gcgcgcgcac
     1921 tgtccccgcg tcgtgctgtg cagcgtgtcc gccttcggcg acgggccgct cggggccacg
     1981 atgccgggtt acgacgcgct cgtgcaggcg gtttccgggt tgatgagctt caccggccac
     2041 ccgcacacgc ctcccacgcg gctggcgccg tcggtgctcg acctcacgac gggcatgtgg
     2101 gcgctcatcg gcatcatggc cgctctcacc cgccgtgcag cgggcggcgg cgccgagcac
     2161 gtgcgccctg cac
//
LOCUS       DASWZT010000214         5623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_54879, whole genome shotgun sequence.
ACCESSION   DASWZT010000214 DASWZT010000000
VERSION     DASWZT010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54879"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="VGE92_05845"
     CDS             <1..1057
                     /locus_tag="VGE92_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003123890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEY1313374.1"
                     /translation="LLAASPSGTSGVDWTQATKIGETLLISPLIGFSAAYLLLSILKA
                     TIRIPALYQEPEGDKPPPLWIRGLLILTCTGVSFFHGGNDGQKGMGLIMLILIGTVPA
                     AYALNRTMHEEDVANFVTISAQAQTALSHYAGGAAPADAHAAALEVVRARAVSPQGVT
                     ALAALTGSISTQVDRYHSLDKVPAGSMINIRNDMYLVSEGLRFLSKAKALAVSEADKS
                     TLAAYKKQLDHSTKFIPTWVKVAVAIALGLGTMIGWKRIVVTVGERIGKTHLTYAQGA
                     SAEIVAMATIGAAEMYGLPVSTTHVLSSGVAGTMVANHSGLQWATVRNLLMAWVLTLP
                     VSITLAGALYWLLRNVL"
     gene            complement(1028..1516)
                     /locus_tag="VGE92_05850"
     CDS             complement(1028..1516)
                     /locus_tag="VGE92_05850"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEY1313375.1"
                     /translation="MPASRVLFVCLGNICRSPTAEGVFRHLLEREAPQLAVEVDSAGT
                     ADYHVGDAPDIRSQRAARQRGIDLSGLRARQVTSEDFANFDFILAMDRSNLRELEAMR
                     PPHSRAQVRLFMEYAPELARLEVPDPYYTGAYGFEDVLDLATAASRGLLAVLQNVAQQ
                     PV"
     gene            complement(1518..2282)
                     /gene="kdsB"
                     /locus_tag="VGE92_05855"
     CDS             complement(1518..2282)
                     /gene="kdsB"
                     /locus_tag="VGE92_05855"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF009905.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEY1313376.1"
                     /translation="MFHVIIPARFSASRLPGKPLLLIGDQPLIQWVWQRAQASGAASV
                     TIATDDVRILDAAIGFGANCVMTSRQHTSGTDRLAEAAQAKGFGADEIVVNLQGDEPM
                     MPSTVIEQVAQALERHPHMDIATAVAPIQSLEEFLDANCVKALRAQDGAALYFSRAPV
                     PWPREHVAAGRPTDYAGAWRHIGIYAYRVRSLLQFAAWQPTPLEQAERLEQLRALEHG
                     MRIHLVALSQTPPAGVDSPEDLARVRAHVATDAAAR"
     gene            complement(2297..2491)
                     /locus_tag="VGE92_05860"
     CDS             complement(2297..2491)
                     /locus_tag="VGE92_05860"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEY1313377.1"
                     /translation="MDKRLLELLVCPICKGPLRSVGAGNALELVCRPDRLAYAVREGI
                     PAMMPEEARSLAADDPLLER"
     gene            complement(2491..3501)
                     /gene="lpxK"
                     /locus_tag="VGE92_05865"
     CDS             complement(2491..3501)
                     /gene="lpxK"
                     /locus_tag="VGE92_05865"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEY1313378.1"
                     /translation="MSVQSWLIRIWYERATPPWWLKPLALTYGAIARSRRYLYAKRLR
                     ASARMPWPVIVIGNLSVGGTGKTPLVCWLAARLTERGFRPGVVTRGYGGSARGVLLVG
                     PSDDPDVVGDEPLLLARRTGVPVAAGRDRPAAARLLGAAGCNVVLSDDGLQHYALARD
                     CEIVVIDGVRRFGNGWLLPAGPLREPPSRLATADGVVVNGGQASPDGTFSMRLDAQRV
                     RSVIGNSAKGLDEFAGSKVHALAGIGNPQRFFSMLRAHGLEVIAHELADHARLRAADI
                     SFDDGRTVLMTEKDAVKCTAIAGPQHWYVPVAANFDVEDSKRLLDIVIQAIQRRASRA
                     GV"
     gene            complement(3518..5281)
                     /gene="msbA"
                     /locus_tag="VGE92_05870"
     CDS             complement(3518..5281)
                     /gene="msbA"
                     /locus_tag="VGE92_05870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEY1313379.1"
                     /translation="MNAAAAAGPTPAGDSWSIYRRLVKYATPYIGVFLIGVLGAALFA
                     ASNAALAYLVRNFIKGAFLVKDPAILWEVPIGVVGLFTLRGLGDYVQTYFPSWVGRQV
                     IKGLRRDVFAHYLRLPTAYLDRQQSGHLLSKLTNNIELVAAAATSAAISLISDSLTLV
                     ALLITLFYMNWRLATFCIVAAPVIAWLMKIANSSFRRYSERIQNSMGDITRVAKEAID
                     AHRLIKIFNAEDHQADRFEQANEHNRASNMKLIRARAVSNPVVQFIAALALGSVLYVA
                     IRQVFTHHMQSDEFFGFLTALMLIPAPLRSLVNMSGPLQQGIAAGNSVFEILDQPTEG
                     SGGTRQLTRARGDIEFRNVSFAYGGDKGDVLRNVSFHVRPGETVAIVGPSGGGKTTLV
                     GLIPRFYDAQTGSVLLDGVDVREYRLTDLRSQVSFVSQDVVLFNDTIRNNIAFNAVGK
                     TDAEVEAAARAANVMEFVAQQPQGFDTPLTDRGQNFSGGQRQRISIARALLKDSPVLI
                     LDEATAALDNESERRVQQELGVLMQGRTTLVIAHRLSTVEGADRIIVLEHGRIAESGT
                     HQELLARGGVYAALHGMLLSA"
     gene            complement(5278..>5623)
                     /locus_tag="VGE92_05875"
     CDS             complement(5278..>5623)
                     /locus_tag="VGE92_05875"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY1313380.1"
                     /translation="IFFMVSTTFNQEGRVRVQLPQASEVPVPRGSHEQLVITVTAEGG
                     YRINERSLINASPDTLRAALLKEAGTDRGPITIRADARSTHQAVVTAMDVAGRMGFSQ
                     LNIATVHQEAAP"
BASE COUNT         1046 a         1862 c         1743 g          972 t
ORIGIN      
        1 gctgttggcg gcgagcccct ccggcaccag cggcgtcgac tggactcagg cgaccaaaat
       61 cggcgaaacg ctgttgatct cgcccctgat cggctttagc gccgcctacc tgctgctgtc
      121 gattctcaaa gcgaccatca ggatcccggc cctgtaccag gagccggagg gcgacaagcc
      181 gccaccgctg tggattcgcg gcctgttgat cctaacctgc accggcgtga gcttcttcca
      241 cggcggcaac gacggccaga agggcatggg gctgatcatg ctgattctca tcggcacggt
      301 cccggcggcc tacgcactca atcgcaccat gcacgaggaa gacgtcgcga atttcgtgac
      361 aatttcggca caggcacaaa ccgcgttgtc gcattacgcc ggcggcgcgg cgcccgccga
      421 cgcgcatgcc gcggccctgg aagtggtgcg cgcccgagcc gtgtcgccgc aaggcgtgac
      481 agcgttggcc gctctgaccg ggtcgatctc gactcaggtc gaccggtatc actccttgga
      541 caaggttcct gccggatcaa tgatcaacat tcgcaacgac atgtacctgg tgagcgaggg
      601 cctgcgcttc ctgagcaagg cgaaagcgct ggccgtctcg gaggccgaca agagcaccct
      661 ggctgcctat aaaaagcagc tcgaccattc gaccaaattc attccgacct gggtcaaagt
      721 ggcggtcgcc atcgccttgg gcctgggcac aatgatcggc tggaagcgca tcgtagtcac
      781 cgtcggcgag cgaatcggta agactcatct gacgtacgcg caaggcgcct cggcggaaat
      841 cgttgccatg gcgacgatcg gcgccgccga aatgtacggc ttgccggtca gcacgacgca
      901 cgtcttgtcg tcgggcgtcg ccggcacgat ggtggcgaat cactccggct tgcagtgggc
      961 gacggtacgc aatctgctca tggcctgggt gctcacgctg ccggtctcga tcactctcgc
     1021 cggcgcctta tactggctgc tgcgcaacgt tctgtaggac cgcaagcagg ccgcgcgagg
     1081 cggcggtggc cagatccaat acgtcctcga agccgtacgc tccggtgtaa tacgggtcgg
     1141 gcacctcgag gcgcgccaac tccggcgcat attccatgaa cagccgcact tgcgcgcgcg
     1201 agtgcggcgg ccgcatggcc tcgagctcgc gcaggttgct tcggtccatg gccagaatga
     1261 aatcgaagtt cgcgaaatcc tcgctcgtca cttgccgcgc gcgcaggccg ctcaaatcga
     1321 tgccgcgctg tctcgcggca cgttgactgc ggatgtcggg cgcatcgccg acgtgataat
     1381 ccgctgttcc cgcagaatca acctcgacgg ctagttgcgg cgcctcgcgc tcgagaagat
     1441 gccgaaatac gccctccgcc gtcgggctgc ggcagatgtt cccgaggcaa acgaaaagca
     1501 ctctcgatgc gggcatctca gcgtgccgcc gcgtcggtgg ccacgtgggc gcgcacccgg
     1561 gccaaatcct cgggcgagtc gacgcccgcc ggcggtgtct gtgacagcgc caccaaatga
     1621 atccgcatcc catgttccag cgcccgtagc tgctcgagcc gctccgcttg ctccagcgga
     1681 gtcggctgcc acgccgcgaa ttgcagcagg ctgcgtacgc ggtaggcata gattccaatg
     1741 tgccgccagg cgccggcgta atcggtgggc cgcccggctg cgacgtgctc gcgcggccac
     1801 ggcaccggtg cgcggctgaa gtacagcgct gcgccgtcct gcgcgcgcaa tgctttgacg
     1861 cagttggcat ccagaaattc ttcgagcgac tggatcggcg cgacagcggt ggcgatatcc
     1921 atgtgcgggt gccgctcgag cgcctgcgcc acttgttcga tcaccgtcga cggcatcatc
     1981 ggttcgtcgc cttgcagatt cacgacgatc tcatccgcac cgaagccctt cgcctgggcc
     2041 gcctcggcga gccgatcggt tcctgaggtg tgctgccgcg aagtcatcac gcagttggcg
     2101 ccgaatccga tggcggcatc aagaatgcgc acatcgtccg tcgcgatggt cacgctcgcc
     2161 gcgccgctcg cctgcgcgcg ctgccacacc cattggatca gcggctgatc gccgatgagg
     2221 agcaagggct tgcctggcag gcgcgaagcg ctgaagcgcg cgggtatgat cacgtgaaac
     2281 atggtctgcg ggcctcctaa cgctcgagca gcggatcgtc ggcagccaaa ctgcgggcct
     2341 cctccggcat catcgccgga atcccctcgc gcaccgcata tgccagccga tccggccggc
     2401 acacgagttc gagcgcatta ccggcgccga cgctgcgcaa cggccccttg cagatcggac
     2461 agaccaacaa ctcgagcaag cgcttgtcca ttacaccccc gcgcgcgacg cgcgcctctg
     2521 aatagcctga atgacgatat cgagcaaccg cttggaatct tcaacgtcga aattggctgc
     2581 gacgggaaca taccagtgct gcggaccggc gatcgcggtg cattttacgg catctttctc
     2641 ggtcatgagc accgtgcgcc catcgtcgaa cgagatgtcg gcggcccgca aacgcgcatg
     2701 atccgccagc tcgtgggcga ttacctcgag gccgtgcgcg cgcagcatgc tgaaaaaccg
     2761 ctgcggattg ccgatgccgg cgagtgcatg cactttgctg cccgcaaatt catccaaacc
     2821 cttggcagag ttgccgatca cggagcgcac gcgttgcgcg tcgagccgca tgctgaaagt
     2881 gccgtcgggc gacgcttggc cgccgttcac cacaacgcca tccgcggtcg ccaatcgtga
     2941 gggtggctcg cgcaatgggc cggccgggag cagccaacca ttgccgaagc ggcgtacgcc
     3001 gtcgatgacg acaatctcgc agtctcgtgc cagcgcgtag tgttgcaatc catcgtcgct
     3061 cagtacgacg ttgcatcccg cagccccgag tagccgtgcc gccgccggac gatcgcgccc
     3121 ggcggcgacg ggcacgccgg tgcgccgcgc gagcaacagc ggctcatcgc ccacgacgtc
     3181 gggatcatcg gacggcccaa ccagaagcac gccgcgcgcg gagccgccat agccgcgcgt
     3241 aacgaccccc gggcgaaaac cgcgctccgt caggcgtgcg gccagccagc agaccagggg
     3301 ggtcttgccg gttccgccga cactcagatt gccgatgacg atcaccggcc aaggcattcg
     3361 tgccgatgcg cgcaggcgct tggcgtagag gtagcgccgc gaccgggcaa tcgcaccata
     3421 ggtcagcgcc agcggcttga gccaccaggg cggcgtcgcg cgttcgtacc agatgcgaat
     3481 gagccaggat tggaccgaca ttttcggggc gccagaatca agcgctcaac agcatcccgt
     3541 gcaatgccgc gtacacgcca ccgcgcgcca gcagctcctg atgcgtgccg ctctcggcga
     3601 ttcggccgtg ttccagaacg atgatgcgat ccgcgccttc gaccgtggag agccggtgcg
     3661 caatcaccaa tgtcgtgcgg ccctgcatca gaacgcccaa ctcctgctgc acgcgccgct
     3721 ccgactcgtt gtcgagcgcc gccgtagcct cgtcgaggat cagtaccggc gaatctttga
     3781 gcagcgcgcg cgcgatcgaa atccgctgcc gctgtccgcc cgagaagttt tggccgcggt
     3841 cggtcaacgg cgtgtcgaaa ccctgcggct gctgagcgac gaactccatc acattggcgg
     3901 cacgcgccgc ggcttcgact tcggcgtcgg tcttgccgac tgcgttgaag gcgatattgt
     3961 tgcggatggt gtcgttgaac agcactacat cctggctgac gaagctcact tgagagcgca
     4021 aatccgtcag gcgatactcg cgcacgtcga caccgtccaa cagcaccgaa ccggtctgcg
     4081 catcgtagaa gcgcgggatc aggccgacca aggtggtctt gccgccgccg gacggcccga
     4141 cgatggcgac agtctcgccc ggacgcacgt gaaacgacac gttgcgcagc acatcgccct
     4201 tgtcgccgcc gtatgcaaaa gacacgttgc gaaactcgat atcgccgcgc gcacgagtca
     4261 actgccgcgt gccgccgctg ccctcggtcg gttgatccaa aatctcgaat acgctgttgc
     4321 ccgctgcgat gccttgctgc aacggtccgc tcatgttgac caagctgcgc agcggcgccg
     4381 gaatcagcat caacgccgtc aagaatccaa aaaactcgtc ggattgcatg tggtgggtaa
     4441 acacctgccg aatcgccaca tagagcacgc tgccgagggc cagcgctgca atgaattgca
     4501 cgaccggatt gctgacggct cgcgcacgga tcagcttcat gttggacgcg cggttgtgct
     4561 cgttggcctg ctcgaagcgg tcggcttgat gatcctcggc gttgaatatc ttgatcagac
     4621 ggtgcgcatc gatcgcctcc ttggcgaccc gcgtgatatc gcccatggaa ttttgaatgc
     4681 gctcgctata gcggcggaag ctgctgttgg caattttcat caaccaggca atcacgggcg
     4741 ccgcgacgat gcagaacgtc gcgagccgcc agttcatata gaacagggtg atcagcagcg
     4801 ccaccagggt cagactgtcg ctgatgagcg aaatcgcagc actggtcgcg gcagcggcga
     4861 ccaattctat gttgttggtg agtttcgaca acaggtggcc cgactgttgg cggtcaagat
     4921 atgcggtcgg cagccgcaga tagtgcgcga acacgtcgcg ccgcagcccc ttgatgacct
     4981 gccggcccac ccagctcgga aagtacgtct gcacgtaatc tcccaggccg cgcagggtga
     5041 ataatccgac cacgccgatc ggcacctccc acaagatcgc gggatctttg accagaaacg
     5101 cgcccttgat gaaattgcga accaggtacg cgagcgctgc attcgacgcc gcaaacagag
     5161 cggcacccag cacgcctatc agaaacacgc cgatatacgg ggtcgcgtat ttcaccagcc
     5221 gccgatagat cgaccaggaa tcgccggcag gtgtcgggcc cgcagcggct gccgcgttca
     5281 aggcgccgcc tcctggtgca cggtcgcgat attgagctgc gagaatccca tgcgcccggc
     5341 cacatccatc gcggttacca ccgcctggtg cgtgctgcgt gcatcggcgc ggatggtgat
     5401 aggaccgcga tcggtgccgg cctccttgag taatgccgcc cgcaaagtgt ccgggctggc
     5461 gttgatcagg ctgcgctcgt tgattcgata gccgccttcg gcagtcaccg tgatcaccag
     5521 ctgctcatgc gaaccacgcg gcaccggcac ttcgctcgcc tgcggtaatt gaacccgcac
     5581 gcgtccttct tggttgaagg tcgtcgatac catgaagaag atc
//
LOCUS       DASWZT010000215         6498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_69250, whole genome shotgun sequence.
ACCESSION   DASWZT010000215 DASWZT010000000
VERSION     DASWZT010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6498
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69250"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..1522
                     /locus_tag="VGE92_05880"
     CDS             194..1522
                     /locus_tag="VGE92_05880"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEY1313381.1"
                     /translation="MRITIFGSGYVGLVTGACLADAGNQVVCVDVDDRKIAMLKRGEL
                     PIHEPGLDEMVKRNFQAGRLRFTTSAKEGVAHGLFQIIAVGTPPDEDGSADLRYVLTV
                     ARAIGEHMTGYKVVTTKSTVPVGTADRVREAVSEVLAARGLSVEFDVVSNPEFLKEGA
                     AINDFMRPDRVIVGTDSERATELLRSLYEPFTHNRDRMIVMDVRSSELTKYAANAMLA
                     TKISFMNELANLAEHVGADIEAVRLGIGSDPRIGYAFIYPGVGYGGSCFPKDVQALKR
                     SADEVGFEASVLTAVESVNNRQKHILFNKIKAHFGELRGRTVALWGLAFKPNTDDMRE
                     ASSRVLMESLWSAGVTVRAYDPVAMHECLRIYGERDDLILCKSSFEALAGADALAIVT
                     EWREFRSPDFDAIKAALRTPAIFDGRNLYDPGQMARAGFSYYAIGRGKPK"
     gene            1530..2954
                     /locus_tag="VGE92_05885"
     CDS             1530..2954
                     /locus_tag="VGE92_05885"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HEY1313382.1"
                     /translation="MTLLVPVILSGGAGTRLWPLSREMYPKQLLALTGKRTMLQDTAT
                     RLAGISGAHAPIVVCNEAHRFTVAEQIRALGIKPSAILLEPVGRNTAPAVALAALKAL
                     EIDPEATLVVAPADHVIRDARGFQTAAETAAALAQDGKLVTFGIVAHAPETGYGYIRR
                     GEGEGPGYPVAQFIEKPPLDVAAQFVASGDYFWNSGMFVFKASRYLAELGTYAPDILE
                     ASQAALRAAKTDLDFVRIDKAAFAKCRSESIDYAVMEKTREALVLPLDAGWSDVGSWS
                     SLFDALPADEEGNVLQGDVMVHDTHDCYVHSTSRLVAAVGMDDHIIVETKDAILVAPK
                     ERVQDVKELVAKLKKSGRSESSLHREVFRPWGSYDSIDNGERFQVKRLSVKPGGVLSL
                     QMHHHRAEHWIVVQGTARITCNDKTFLLSENESTYIPIGATHRIENPGKLPLHIVEVQ
                     SGSYLGEDDIVRFEDNYGRQGTNT"
     gene            2951..4336
                     /locus_tag="VGE92_05890"
     CDS             2951..4336
                     /locus_tag="VGE92_05890"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HEY1313383.1"
                     /translation="MSSITCFKAYDLRGRIPAELNEDVAYRVARGYAQFLARGADALR
                     IVVGRDIRLSSTALTDAVCRGLTDSGVDVFDIGVCGTEGVYFATFDGRYDGGIMITAS
                     HNPPDYNGMKFVREQSKPISGDNGLQEIRGFAERGEFPLPQRAGVRRHIDTMQAYVAH
                     LKSYVDLRRLKPLKIVVNAGNGGAGLVIDALEADLPFKFIKVHHEPDGNFPNGIPNPM
                     LEENRAPTVEAIRRHGADFGVAWDGDFDRCFFFDEHGGFIEGYYIVGLLASVLLRGHP
                     GGNVVHDPRLTWNTIEVVKAGGGVAVLSKSGHAFIKQRMREVDGLYGGEMSAHHYFQR
                     FAYCDSGMIPWLIVAQILSESGLTLSQLVGERIALFPVSGELNYRVPDAQATIAAFEA
                     RYAPLALVLDRTDGVSFEFADWRFNLRSSNTEPLIRLNVEARGSVDLMRAKTAELLTV
                     LTAQGAIAADH"
     gene            4343..5335
                     /locus_tag="VGE92_05895"
     CDS             4343..5335
                     /locus_tag="VGE92_05895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY1313384.1"
                     /translation="MAVAPKLSVVIPVYNEEAVLPTLFSRLYPTLDALDISYEIVFVN
                     DGSKDRSVAVLRQQYELRPRQTRVVLFHANFGQHSAVMAGLKYARGEYVVTLDADLQN
                     PPEEIGKLVAMLDQGYDYVGTIRQQRQDSLWRRWFSKRINRLRELITPVRITDQGCMM
                     RGYARSVVTALNQTREVNTFIPALASLYAMRPIEVPIAHEERFAGKSKYSMYSLIRLN
                     FDLITGFSVVPLQLFSMTGMLVSMISAVLFVVLLVRRIWLGSEAEGLFTLFAFVFFLI
                     GVALFGIGLLGEYVGRIYAQVRERPRYIVQAVLEDEPNPIERRVNTEAFSRGAP"
     gene            5332..6258
                     /locus_tag="VGE92_05900"
     CDS             5332..6258
                     /locus_tag="VGE92_05900"
                     /inference="COORDINATES: protein motif:HMM:NF005414.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HEY1313385.1"
                     /translation="MTRAVVFAYSEVGVRCVRELLAQGVEIPLLFTHADDPAESQWFG
                     SVQQLASAHHLRVETPDNPNTPQWIGAGSAARADFLFSFYYRYMLDKAWLTVAARGAL
                     NMHGSLLPKYRGRAPVHWAILNGEQATGASLHYMVEKPDAGALVDQQSVPILQNDTAL
                     DVSIKVAEAAVEVLRRSLPKLIDGSAAAIPLDLGSGSYFGRRRPEDGRIDWSRTARAV
                     HDLVRAVAPPFPGAFTEVNGCRLAILETRVDARPTRFSRQAPCLYAADGEWYVDCVDG
                     RRLCITRLAIDGKFVTPEHAPPALMRQPLALG"
     gene            6303..>6498
                     /locus_tag="VGE92_05905"
     CDS             6303..>6498
                     /locus_tag="VGE92_05905"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY1313386.1"
                     /translation="MKNVLILGVNGFIGHHLSNYIVAHTDWHIYGMDLQSERVSALLG
                     NPRFHFVEGDITINLEWVEYH"
BASE COUNT         1206 a         1878 c         2099 g         1315 t
ORIGIN      
        1 ctcgccgcga gggcgcgaac cgcggcgagt atttccgagg tgacttgcgt ggcgaacttc
       61 tctttggcgg cggacatcgt tttccccagt tgatgcatca tgatacatca tgatggatca
      121 tgatgcttta cttgaagtcc caaaaccgct agtcttccgc ggctcttttc ctagctaaat
      181 cagaatatta cgcatgagaa ttacgatttt tggttccggc tatgtgggcc tggtgacagg
      241 tgcatgtctg gccgacgccg gcaatcaggt ggtgtgcgtc gatgtcgacg atcgtaaaat
      301 cgcgatgctc aagcggggcg aactccccat ccacgagccc ggtctcgacg agatggtcaa
      361 acgcaatttc caggcggggc gattgcgctt caccacgagt gccaaggaag gcgtggcgca
      421 cggtctgttt caaatcatcg cggtgggcac cccgcccgac gaggacggct cggcggacct
      481 gcgctatgtg ttgacggtgg cgcgcgcgat cggcgagcac atgaccggct acaaagtggt
      541 aaccacgaaa tccaccgtgc cggtgggcac cgccgatcgg gtacgcgagg cggtcagcga
      601 ggtgctggcc gcgcgcgggc tcagcgtcga attcgatgtc gtttcgaatc ccgaatttct
      661 caaggaaggg gcggccatca acgatttcat gagacccgac cgggtgatcg tcggtaccga
      721 ctcggaacgc gccaccgaac tgttgagatc tctgtacgag ccgttcaccc acaatcgcga
      781 ccggatgatc gtcatggatg tgcgctcatc ggaactgacc aagtacgctg ccaacgccat
      841 gctcgcgacc aaaatcagct tcatgaatga attggccaat ttggccgaac atgtcggcgc
      901 ggatatcgag gcggtacggc tcggcatcgg ctcagacccg cgcatcggtt acgcattcat
      961 ctatccgggc gtgggctacg gcgggtcctg ttttcccaag gatgtgcagg cgctgaaacg
     1021 atcggcggat gaagtgggtt tcgaggccag cgttctgacg gccgtggaga gcgtcaacaa
     1081 tcgtcagaag cacattctgt tcaacaagat caaggcgcat tttggcgagt tgcgcggcag
     1141 gaccgttgcg ttatggggcc tcgccttcaa gcccaatacc gacgacatgc gcgaagcgtc
     1201 gagtcgcgtg ctgatggagt cgctctggtc ggccggcgtg acggtgcgtg cctacgatcc
     1261 ggttgcgatg cacgagtgtc tgcggattta cggcgagcgc gacgatttga ttctgtgcaa
     1321 atccagcttt gaggcgctgg ccggtgcgga tgcgctcgcg atcgtgaccg aatggcgcga
     1381 gtttagaagt ccggatttcg acgcgatcaa agccgcgctg cgcacgccgg ccattttcga
     1441 cgggcgaaat ctctatgacc ccgggcaaat ggcacgggcc ggcttcagtt attatgcaat
     1501 cggccgtggc aaacccaagt gagtgaattg tgaccctatt ggttccggtg attttgtcgg
     1561 gcggcgccgg caccaggctg tggcctctgt cgcgcgagat gtatccgaag cagctgcttg
     1621 cactcaccgg caagcgcacc atgctgcaag atacggcgac gcgtctggcg ggaataagcg
     1681 gggcgcacgc ccccatcgtg gtgtgcaacg aagcacatcg ctttacggtg gcggaacaga
     1741 tccgggcttt gggcatcaag ccgtcggcga ttctgctcga gccggtgggc cgcaataccg
     1801 cgccggccgt cgcgctggcg gcgctcaagg cgctcgagat cgatcccgaa gcgaccctgg
     1861 tcgtcgcgcc agccgatcat gtgattcgcg atgcccgagg gtttcaaacg gcggccgaaa
     1921 cagcggccgc tttggcgcag gacggcaagc tcgtcacatt cggcatcgtc gcccacgcgc
     1981 cggaaaccgg ctacggctat atccgccgcg gcgaggggga agggcccggc tatccggtgg
     2041 cgcaattcat cgagaagccg ccgctcgacg tcgcggcgca gttcgtggcg tccggcgatt
     2101 acttctggaa cagcggcatg ttcgtgttca aggcgagccg ctacctcgcg gaactgggca
     2161 cgtatgctcc cgacattctc gaggcgtcgc aagcggcgct gcgcgcggcc aagacggatt
     2221 tggacttcgt gcgcatcgat aaagcggcat tcgcgaaatg ccgcagcgaa tcgattgact
     2281 atgccgtcat ggagaagacg cgcgaagcgc tggtactgcc gctcgacgcg ggctggagcg
     2341 acgtcggttc gtggtcgtcg cttttcgacg cgctgccggc cgacgaggag ggcaacgtat
     2401 tgcagggcga tgtcatggtg cacgacacgc acgattgcta tgtgcattcg accagccgtt
     2461 tggtggcagc ggtcggcatg gacgatcaca tcatcgtcga aaccaaggat gccattttgg
     2521 tggcgcccaa ggaacgcgtt caggatgtca aggagttggt tgccaagctc aagaagtccg
     2581 ggcgcagcga gtcgtcctta catcgcgaag tattcaggcc gtggggcagc tacgacagca
     2641 tcgacaacgg cgagcgcttc caagtgaagc gattgtcggt caagccgggc ggggtcctgt
     2701 cgctgcaaat gcaccaccat cgggccgaac attggatcgt ggtgcagggc acggcccgaa
     2761 ttacctgcaa cgacaagacg tttctcctgt cggagaacga atcgacctac attccgatcg
     2821 gcgccacgca ccgcatcgag aatcccggca agctgcccct gcacatcgtc gaggtgcagt
     2881 cgggttcgta tctcggcgag gatgacatcg tgcgcttcga agacaattac ggccgtcaag
     2941 gaaccaatac ttgagttcga tcacctgctt caaggcgtac gatttacgcg gccgaattcc
     3001 ggccgagttg aacgaagatg tggcttaccg ggtcgcgcga ggttacgcgc agttcttagc
     3061 gcgcggcgca gacgccttgc gtatcgtcgt cggacgcgat atccgcttgt cgagtaccgc
     3121 gctcaccgat gccgtgtgcc gcggtttgac ggacagcggc gtcgatgtgt tcgacatcgg
     3181 ggtgtgcggg acggagggcg tgtattttgc gaccttcgat ggccgctatg acggcggcat
     3241 catgattact gcgagccaca acccgccgga ttataacggc atgaaattcg tgcgtgagca
     3301 atcgaagccg atcagcggcg acaacgggct gcaggagata cgcggattcg ccgagcgcgg
     3361 cgagtttccg ctgccgcagc gtgccggcgt gcgccggcac atcgacacca tgcaagccta
     3421 tgtggcgcat cttaagtcct atgtggatct gcggaggctc aagccgctca aaatcgtggt
     3481 caacgccggc aatggcggcg cgggcctggt catcgacgcc ttggaagccg atttaccctt
     3541 caagttcatc aaagtgcacc acgagccgga cgggaatttc cccaacggca taccgaatcc
     3601 gatgctcgag gagaatcgcg cgcccacggt agaggcgatc cgccgtcacg gtgcggactt
     3661 cggagtcgcc tgggatggcg atttcgaccg ctgctttttc ttcgacgaac atggcggctt
     3721 catcgaggga tactatatcg tcggactgct ggcatcggtg ctgctgcgcg gtcatccggg
     3781 cggcaacgtg gtgcacgatc cgcgtctcac ctggaacacg atcgaagtgg tgaaggcggg
     3841 cggcggcgtc gcggtgttgt cgaagtcggg ccatgccttc atcaagcagc gcatgcgcga
     3901 ggttgacggc ctgtacggcg gcgagatgag cgcgcaccat tattttcaac gtttcgcgta
     3961 ttgcgacagc ggcatgattc catggctcat tgtcgcgcaa atcctcagcg aatccggttt
     4021 gacgctgtcg cagctggtgg gcgaacggat tgccctgttt ccggtcagcg gcgaactcaa
     4081 ttatcgggtt cccgatgcgc aggcgaccat cgccgccttc gaggcgcgtt atgcgccgct
     4141 ggcgttggtg ctcgatcgca ccgatggcgt ttccttcgaa ttcgcggatt ggcgcttcaa
     4201 cttgcgcagc tccaataccg agccgctgat acggctcaat gtcgaggcgc gtggttcggt
     4261 cgacctgatg cgcgccaaaa ccgcggaact gctgacggtt ttgaccgcgc agggcgcgat
     4321 cgcggccgac cactgaaccg atatggccgt cgcccccaaa ttgagcgtcg tgattccggt
     4381 ttacaacgaa gaggcggtgc tgccgaccct gttttcgcgg ctctatccga cgctcgatgc
     4441 gctcgatatc tcgtacgaaa tcgtattcgt caacgacggc agcaaggatc gttcggtggc
     4501 ggtattgcgc caacaatacg agttgcggcc gcggcaaacc cgcgtggtgt tgttccatgc
     4561 gaatttcgga cagcattcgg ccgtcatggc ggggcttaag tacgcgcgcg gcgaatatgt
     4621 ggtcaccttg gacgcggatc tgcagaatcc gccggaggag atcggcaaac tcgtcgccat
     4681 gctggatcag ggctacgact acgtcggcac catacgtcag cagcggcaag actcgttgtg
     4741 gcgccgctgg ttctcgaagc gcatcaaccg gctgcgcgag ttgatcacgc ccgtgcgcat
     4801 caccgatcag ggctgcatga tgcgcggtta cgcgcgctcg gtggtcactg cgctcaatca
     4861 gacgcgcgag gtcaatacct tcattccggc gctggcatcg ctctatgcga tgcggcccat
     4921 cgaggtgccg atcgctcacg aggagcgctt cgcgggcaaa tcgaagtatt cgatgtacag
     4981 cttgattcgt ctgaatttcg atctgatcac cggattctcc gtcgtgccgc tgcagctgtt
     5041 ttcgatgacc ggcatgctgg tttccatgat ttcggcggtg ctgttcgtgg tactgctggt
     5101 gcgccgaatc tggctgggct cggaggccga gggtctgttc accctgttcg cgttcgtatt
     5161 cttcctgatc ggcgtggcgc tgttcggtat cggactcctc ggtgagtacg tcgggcgcat
     5221 ctacgcccag gtgcgtgaac ggccgcgcta catcgtgcag gcggtcctgg aggatgagcc
     5281 gaacccgatc gagcgccgcg tcaatacgga ggcatttagt cggggcgcgc cgtgactcgc
     5341 gccgtggtat tcgcctacag cgaggtcggg gttcgctgcg tgcgcgaact gttggcgcag
     5401 ggcgtggaga ttccgttgtt gttcacgcat gccgatgatc cggccgagag ccagtggttc
     5461 ggaagcgtgc agcaattggc ctcggcgcat catctgcggg tcgaaacacc ggacaacccg
     5521 aataccccgc aatggatcgg cgccggcagc gccgcccgtg cagatttttt gttctccttt
     5581 tactatcgct acatgttgga caaggcgtgg ttgacggtcg cagcgcgcgg cgcactcaac
     5641 atgcacggat cgctgctgcc caagtatcgc ggccgggcgc cggtgcactg ggcgatcctc
     5701 aacggcgaac aggcgaccgg agcgagcctg cattacatgg tggagaagcc cgatgcgggc
     5761 gcgctcgtgg atcaacagtc cgtaccgatt ttgcagaatg acacggcgct cgacgtgtcg
     5821 atcaaagttg ccgaggctgc cgttgaggtg ctgcggcgca gtctgccgaa gctgatcgat
     5881 ggaagcgcag cggcgattcc gctcgatctt ggcagcggct cgtatttcgg ccggcgacgc
     5941 cccgaagatg gccgtatcga ttggagccgc acggcgcgcg cggtgcacga tctggtgcgc
     6001 gccgtggcgc cgccgtttcc gggtgccttt accgaggtaa acgggtgtag gcttgccatt
     6061 ctcgagaccc gggtcgacgc gcggccgaca cgtttttcaa ggcaggcgcc gtgtctgtac
     6121 gccgcggacg gcgagtggta cgtcgattgc gtggacgggc gcaggctgtg cattacgcgg
     6181 ctcgcgatcg acggcaaatt cgtgacgccc gagcacgcgc cgccggcgtt gatgcgccag
     6241 ccgttggcgc tcggctgaaa tcccgggacg ggccggacga tttatcaggg taggggcaaa
     6301 ttttgaaaaa cgttctcatt ctcggggtca acggcttcat cggtcaccac ttgagcaact
     6361 acatcgtcgc tcataccgat tggcacatct acggcatgga tttgcaatcc gaacgcgtca
     6421 gcgcgcttct tggcaatccg cgtttccact ttgtggaggg cgatatcacg atcaacctcg
     6481 agtgggtcga gtatcacg
//
LOCUS       DASWZT010000216        14499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_30709, whole genome shotgun sequence.
ACCESSION   DASWZT010000216 DASWZT010000000
VERSION     DASWZT010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14499
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30709"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VGE92_05910"
     CDS             complement(<1..395)
                     /locus_tag="VGE92_05910"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEY1313387.1"
                     /translation="MRRPIGRDRARALLHRGFFCAGGILLIAAFGTDYTYYTTALWQW
                     ANFSAWLITAGLSITLIAVILLLIDFATGRARRLNTGSFILVTAAALLSLVNAFVHSR
                     DAWTSVVPQGILLSAVSTLLLVIVGARGW"
     gene            complement(507..1052)
                     /locus_tag="VGE92_05915"
     CDS             complement(507..1052)
                     /locus_tag="VGE92_05915"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEY1313388.1"
                     /translation="MRHSASVTAAAIFVALAGAAGAASPASVQLAPVDYAFIAQTNLG
                     NHFQIDTGKLGESHARSAEVRDYAKLMDTSHVQVENNLLALLQKMNIEPPQASLLSGA
                     YEGLVKILVDEQGADFDRDYVRSQLDYQHANDALYRWEIQNGSNPALIAYARETLPKI
                     DDHMHRVEALLAAETGATGSR"
     gene            complement(2198..3613)
                     /locus_tag="VGE92_05920"
     CDS             complement(2198..3613)
                     /locus_tag="VGE92_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing
                     glycosyltransferase family protein"
                     /protein_id="HEY1313389.1"
                     /translation="MASIFGKLLKRRAISTSNADQYAAIYAAATQAATARDYERAVSM
                     YDRALTLNPSPEAYYKRANCLKDLGQLDAAIASYNQAIEHKLDYAYAYCNRGVVQQAL
                     GLTSEALISYDRSIALDPADPIAHYNRALLLQECSRWDEALESYERAIAVNPDYADAQ
                     FNRSLAWLFRGEFERGWRGYEWRWKNAARLGIGELKQFTQPLWLGLESVAGKRVLLHN
                     EGGLGDTIQFCRYAPAVAAMGATVYLEAQAPLLGLLSSLPGVSQLIASGGALPEFEYH
                     CPLMSLPLACKTTLETIPAPRRYLRSTPQHVARWRARLPQRARKRIGLAWSGNSKNRL
                     DRHRSIRLAEWIAHLPAEFDYFCLQKDVRQEDRETLDSNPQIGCFDDEELDFVNTAAA
                     CDCMDIVISVDTSVAHLSAALGRPTWILLPFTPDWRWMAARDDSPWYPSAKLYRQSAA
                     GDWSQLLARVAGDLRRELSAG"
     gene            complement(3661..6198)
                     /locus_tag="VGE92_05925"
     CDS             complement(3661..6198)
                     /locus_tag="VGE92_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY1313390.1"
                     /translation="MGSGVDDAMVILDPAGRVTSWNAGGEALYGYIPQEIVGRDFAQL
                     FDNSDTERGRAQQLLDSASLERTDQKVWCRRKDGTRFWAHVVLTAQLNHRGELLGFAG
                     ASRDLSGAALQARRLEHSEAHLQAFLKNAPAATFIKDAAGRYLHVNDQFVNEFGLARH
                     VVLGRTDAQIFAPELARALQIHDAPVLMHGKKTEFQQQRPCNERCRTNLISKFPIHDQ
                     DGRIIGIGGTVTDITEQLLATQAVQRQTMRQSLIAAFGQFALQNPEFEELLIEADRTI
                     TKGLQPQFSRYLSLSADEKMLQFKAGTGWEGAWAEQINFDAVTETEDRFSIGAREAIL
                     VEDFPKGARYPPSPIISDHRIRSAAEVLVWGVNGPYGLLGVYSRSPGAFAQDSVDFLQ
                     GIKNTLAAAIDRRCAEDRLAYLAQFDALTGLPNRNLYLDRLWQTLRQTDRDKRPVGVI
                     FFDVDHFKSINDKLGHSGGDEVLTQIAVRLKKCLRPADTVARLSGDEFALVLDHLIVP
                     EDATMVAERVIATLAKPFHVHGHEVYVTASLGISIAPGDGSNPDALLKNADMAMYRAK
                     QAGRNTYRFFLPEMNARAAERLQIETELRGALARTEFVLHYQPKADINTGRISGFEAL
                     LRWNHPKRGLVPPNEFIPVLEETGLINEVGDWVMRQACQQIRTWQVGGLRDCPIAVNL
                     SARQFARNNLDSTIGKLLHEFQINPNLLEFELTESVLMSDSRDAVQVLDNMKKYGVRL
                     SVDDFGTGYSSLAYLKRFPLDALKIDRAFISHITTDQGDATIAKAIINLAHSLRLKVV
                     AEGVESQAQLDFLRANDCDEMQGYLFAAPMSAADCADAVIDGWRLPL"
     gene            complement(6562..7428)
                     /gene="hypB"
                     /locus_tag="VGE92_05930"
     CDS             complement(6562..7428)
                     /gene="hypB"
                     /locus_tag="VGE92_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HEY1313391.1"
                     /translation="MCVTCGCGNTDEATLTLHGVEHEHVHADGTRHTHVHAHMPEFAA
                     VPFRQQLPVHSHEIAVSVPGRDVHTIQLEAQILSKNQLIAARNRKRLSLNSILALNLV
                     SSPGAGKTTLLERTLTDLKSQVRISVIEGDQATSNDAERIRATGVAAVQINTGTGCHL
                     EADMLERALDALQPASSSLLMIENVGNLVCPALFDLGEHAKVVIFSVTEGEDKPLKYP
                     HMFAAAEVLLLSKTDLLPHIRFDVERAVANALSVNPNLGVFRVSAYNGEGLPQWYGWL
                     KREMLAANCSVA"
     gene            complement(7419..7760)
                     /gene="hypA"
                     /locus_tag="VGE92_05935"
     CDS             complement(7419..7760)
                     /gene="hypA"
                     /locus_tag="VGE92_05935"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEY1313392.1"
                     /translation="MHELSITRSVVAIVSEQAVGQRVTRVRLEIGSLSAILPDAIRFC
                     FDICAADTPLAGAELQIVEIPGLGCCNECGAEVPLETLVGRCPRCGTSSLRLIAGQEL
                     NIKEIEVQSCA"
     gene            complement(7769..8848)
                     /gene="hypE"
                     /locus_tag="VGE92_05940"
     CDS             complement(7769..8848)
                     /gene="hypE"
                     /locus_tag="VGE92_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEY1313393.1"
                     /translation="MNARYDASLSSIAMPATVPVAPCVTMAHGGGGRAMRGLIEKILL
                     PAFYNPLLATLEDQARIPLAELTALGDRLTFTTDTYVVTPLEFPGGDIGKLAVCGTVN
                     DLAVSGARPLFLSCGLVLEEGLSVADLTKIACSMGSAARAAGVAIITGDTKVVERGAA
                     DGVFINTAGIGVVPAGVDIAATRAQPGDAVIVNGNLGDHGVAILLARNELALAAEVIS
                     DCQPLYGLISAMLAVCPNIHCLRDATRGGVATVLNEFASSSNVGIRLREAALPLNETV
                     RGACELLGLDPLYLANEGKLVAIVPGTHAAAVVAAMRSHPAGRDAALIGEVCAQPAGS
                     VILSTAIGGDRVVDMLVGEQLPRIC"
     gene            complement(8845..9996)
                     /gene="hypD"
                     /locus_tag="VGE92_05945"
     CDS             complement(8845..9996)
                     /gene="hypD"
                     /locus_tag="VGE92_05945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEY1313394.1"
                     /translation="MRFADEFRDPEKARTLVAEIARLAVPLAAARRRPLQVMEVCGGH
                     THTIFRYGLKAMLPDDSIEFVHGPGCPVCVLPMGRVDDCVALARNPKVIFTTFGDAMR
                     VPGSQGSLLHAKANGADVRMVYSPLDALHIAHANPQREVVFFALGFETTMPSTALTVL
                     QARREGIANFSLFCNHITIIPTIKAILDSPDLRLDGFLGPGHVSMVIGNRPYEFIASH
                     YRRPIVVAGFEPLDVLQSLWMVIKQISEGRCEVENQYTRIVPQDGNVSALAAVTEVFE
                     LREFFEWRGLGSIDHSGVRMRGEFAAFDAERKFAVPNLKIADPKSCQCGEVLKGVIKP
                     QQCKVFGTACTPEKPLGALMVSSEGACAAYYQYGRIDVKDRRLTAGALT"
     gene            complement(9999..10691)
                     /locus_tag="VGE92_05950"
     CDS             complement(9999..10691)
                     /locus_tag="VGE92_05950"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY1313395.1"
                     /translation="MSSPLQNSQLAGLYPFLAAQRHDEEQMQQVLLESVRQKSDESLA
                     VKQRFFREFAPQVVAAARALAAAYTRGGRLYTMGNGGSSCDSAHIAVEFQHPVTAGRP
                     ALAAHNLANDCATLTAVGNDLGFQHVFLRQVIALLRPQDVLIGVSTSGNSDNLLLAFA
                     AAKQLGATTIGLAGGDGGKMLSSAALDHCLVAPSDSIHRVQETHVAIYHILWDLTHTL
                     LADTRGSAASEL"
     gene            complement(10693..10968)
                     /locus_tag="VGE92_05955"
     CDS             complement(10693..10968)
                     /locus_tag="VGE92_05955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEY1313396.1"
                     /translation="MCLGIPGRIVAITDAGNKLGMVDVCGVRRQINLACVVNELHSVE
                     SCVGDWVLVHVGFAMSRIDEQEAQLTLDLLRQLGEAQSEIEAMRRSN"
     gene            complement(10980..13361)
                     /gene="hypF"
                     /locus_tag="VGE92_05960"
     CDS             complement(10980..13361)
                     /gene="hypF"
                     /locus_tag="VGE92_05960"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEY1313397.1"
                     /translation="MSETAEIIRVRGLVQGVGFRPSMWRLAQRHGLRGWVANDGQGVR
                     MHVCGPAAAVEEFVLNLPGESPPLARIDRIEREPATALPSDAGFFIAASEITAISTGV
                     PPDAASCAECVREIFDPFARRYRYPFTNCTHCGPRLSIVRHMPYDRANTTMGGFAMCA
                     ACAAEYHDPQDRRFHAQPIACHACGPKLTLARADGHVMTLEALSALDEADAACTLLQR
                     GSILAIQGLGGYQLACDATQPDVVARLRRLKQRELKPFALMARDLAVIRRFALVSESE
                     AVLLAGSVAPIVLLTRVANATQEIAGSVAPGLDTWGFMLPNTPLHHLLLKRMQRPIVL
                     TSGNLSEEPQSIDAVDARQRLSGLADYFLEHNRPIARRVDDSVARVVAGRVRVLRRAR
                     GFAPLELALPPGFEAAPAVLGFGGELKNTFCMLRDGAAIVSAHLGDLQDPLTRADYRR
                     ALADYQRFFAFAPEALACDLHPDYSSTQIARGHADDGNLRLLASQHHHAHIAACLADN
                     GVPRAAAPVIGVALDGMGYGEDGSLWGGEFLLADYAGYQRLGTFKRVALLGGEAAVRE
                     PWRNTYAHIQAQMGWATFAMNYADLELYRFLEQQPRALLDGMLKRGLNSPLASSCGRL
                     FDAAAAAMGLARERAWYEGQGAVEMEAAVDRQCLDTEDDRLSYPFPIPRMAGLPYIEP
                     LGMWAALFGDLILHTPVGVMAARFHRGLASSIVRMVEKVAARIDVAPLPPVALSGGVF
                     QNRVLFERVCAGLEAKGFHVLTHDQVPCNDGGLALGQAVIAAARLLQTAARPH"
     gene            complement(13358..>14499)
                     /locus_tag="VGE92_05965"
     CDS             complement(13358..>14499)
                     /locus_tag="VGE92_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313398.1"
                     /translation="VLDTEGPRVCIGGARGEDNLAIPVKPDAFTLFGPRGAGLMGERG
                     PLWISDTGHHRLLGWAQIPCADGVPADWVIGQPDFSREGRNAKGAPSAITLNVPTGIC
                     AVNEGFAVADAWNHRVLIWRRCPTADNIPADVVLGQADFAATESNRGASGASADSLFW
                     PYGVHWDGADLWVADTGNRRVLKWRGLPQQNGRAADLVLGQTSFECRDENGGGAPSAG
                     SMRWPHAITTWSGKLCVTDAGNNRILVWDSPPTVGNAPADWVLGQNDFDLVDHNQSLY
                     WPSAATLHMPYGITAAGPWCICADTANSRLIAWHIEDCKNGSRARALTGQLQFNAKGD
                     NAWQSPTRCSLSWPYGINAQQRMGVIADSGNNRVLLWPLARELAR"
BASE COUNT         2728 a         4504 c         4519 g         2748 t
ORIGIN      
        1 ctccatcctc gcgcgccgac gatgaccagc agcagggtag acaccgcgga cagcaaaatc
       61 ccttgcggta ccacggaggt ccatgcgtca cggctatgca cgaacgcgtt gaccaaagac
      121 agcaacgcgg ccgcggtaac cagaatgaag gaacccgtgt tgagccggcg ggcgcgccct
      181 gtcgcgaagt cgatcagcag taagatgacg gcgatgagcg tgatgctcag gccggccgtg
      241 atgagccagg cggagaagtt tgcccactgc cagagcgccg tcgtgtaata ggtgtagtcg
      301 gtgccgaagg cggctatcag cagtatcccg cccgcgcaga aaaagccgcg atgtagtagg
      361 gctcgtgccc ggtcgcggcc aatcggtctg cgcatctgct cgtcctcctg gtcacccgat
      421 gaaacaactc tgccgcatga ctcttgcgct aacatcgaca actcccaagg ttgcattgtc
      481 cggatttaag gccgctccga ggtcggtcag cgagagcccg ttgcgccggt ctccgcggcg
      541 agcagtgcct ccacgcgatg catgtggtcg tcgatcttcg gcaaggtctc gcgggcatag
      601 gctatgagcg cggggttcga gccgttctgg atttcccagc gatacagagc gtcatttgcg
      661 tgctgatagt cgagttggct acgcacatag tcgcggtcga aatccgctcc ttgctcgtct
      721 acaaggatct taaccaggcc ctcgtacgca cctgaaagta ggctggcctg cggcggctct
      781 atgttcatct tctgcagaag ggccaagaga ttgttctcga cctgtacgtg gcttgtgtcc
      841 atgagcttcg cgtaatcacg tacttccgcg ctgcgagcat gcgattcgcc gagcttgccg
      901 gtgtcgatct ggaagtggtt gccgaggttg gtctgggcga tgaaagcgta gtccaccggc
      961 gcgagctgga cggatgcggg gctggccgcg cctgcagcgc ccgcgagggc gacgaatatc
     1021 gcagcggctg tgactgatgc agaatgtctc atgacggatc tccaatttgc tgattgcggg
     1081 atctattacg cacggcggcc gttacgatcc gatttcgaca gccctcgcgg atatttcaac
     1141 cgaaatgcca ctcgaacacg cgcgatacgt gatttgccgc gatggggctc gcggcgagca
     1201 acgaaatgct gaacatcctt atcatggcct cttagggtcg agatcgatcg ccttctgaat
     1261 ggctgcatgg gcttcgtccc gatcctcttt atagccgccg gctccaaagg ccacgtatcg
     1321 cacccggagc gtgtcgccga gcaagtccca acccggggcg aaattcggat ccagctcgac
     1381 agcgcgccgg cattcgccga tggcttcttg atttgattcg cgggatccga gcttggctgc
     1441 agccagacac ttgagaaagg ccgtataggc gtcggtattt gccgtgggcg tagcgcgcgg
     1501 gcggcgccca tgagcgatac cttgagtgcc gcaacgacgg cgcggtgatc tcgtcctgga
     1561 cctggaatac gtctttgagt tcccggtcgt aggtcttgca gccccgggac cttggcgatc
     1621 tggtcgagaa tttcctcggc gagaccgtcg gagaagtact cctggtcctt cttttctctg
     1681 aggtcggcaa agggtaagac ggcgatggat ttctcgcgcg gccctgccgt gtcggcgggg
     1741 gctgcggcgg ggatttgcgc tggccgtggc ggtgaatgag tcattcggcc cgcaacgaaa
     1801 agcgcggccg cgacgatggc cactgcgacg gcgacgacgg cgggtttacc cgatcgtcgg
     1861 gcgcgacccg atgagttggc cggtggcaag gcgtgaggcg gtatttgtcg agatgagagg
     1921 gacgaacaac gcgcagtccc ggatctgagt gcggattctc cggtcccagg cttcgccgcc
     1981 gcgcagctca ctctgatcaa accagacctc gataccgcgt gccctaagcg catcacaaat
     2041 acgccgtgcc gcctctgcgt cctgcgaggc atagctcagg aataccgccg ttgaaggctc
     2101 agtcatcgat cgctcctgga tggctcaaga cctctgcaag gttgtcgccc gccttactcg
     2161 tctgcgccgc tctcgccgat agccggcgca cctcgaatca tccagccgac aactcccggc
     2221 gtaaatcacc cgcgactcga gccagcaatt gactccagtc gccggctgcg gattgccgat
     2281 acagcttcgc ggatggatac caggggctat cgtcgcgcgc cgccatccag cgccagtccg
     2341 gggtgaaggg cagcagtatc caggtcggtc tgccgagggc cgcgcttaag tgcgccacac
     2401 tggtgtcgac gctaatcacg atgtccatgc agtcgcacgc tgcagcggta ttgacgaagt
     2461 ccagttcttc atcgtcaaaa caaccgattt gcggattcga atcgagcgtt tcccggtcct
     2521 cttggcgcac gtccttttgc aagcagaaat aatcgaactc cgcaggcaga tgcgctatcc
     2581 attcagcgag tcggatgctg cggtgtcggt cgagcctatt tttcgaattg ccgctccagg
     2641 cgagcccaat gcgtttgcgg gcccgctgcg gcagccgcgc gcgccatcgc gcgacgtgct
     2701 gcggcgtgct ccgtaaatac ctccggggag cagggatcgt ctcaagcgtt gtcttgcagg
     2761 ccagcggcaa actcatcaac gggcaatggt actcaaactc gggcagggcg ccccctgagg
     2821 caatcagttg cgaaacgccc ggcaggctcg agagcagacc gagcaggggc gcttgggctt
     2881 cgagatatac agtcgctccc atcgctgcaa ccgccggagc gtagcggcaa aattggatcg
     2941 tgtcgcccaa tcccccttcg ttgtgtagca gcacgcgctt gcccgcgacc gattcaagac
     3001 caagccagag cggctgggta aattgtttga gctctccgat ccccagccgc gctgcattct
     3061 tccagcgcca ttcgtagccg cgccagccgc gctcgaattc gcctcgaaac agccacgcga
     3121 gcgagcggtt gaattgcgcg tcggcatagt cgggattgac agcaatggct ctctcgtaac
     3181 tctcgagagc ctcatcccac cgcgagcact cctgcagtaa caatgcgcgg ttatagtggg
     3241 ctatggggtc ggccggatcg agtgcgatcg agcgatcgta gctgatcaac gcctcgctcg
     3301 tgaggccgag cgcttgttgt actacgccgc gattgcagta agcgtaagcg taatcgagct
     3361 tatgctcgat ggcttggtta tagctggcga tcgccgcgtc gagctgtccg agatctttca
     3421 agcaattggc gcgcttgtag taggcctcgg gcgacgggtt gagtgtcagc gcacggtcgt
     3481 acatgctaac ggcgcgctcg taatcccgag ccgtagcagc ctgcgtggcc gcagcgtaga
     3541 tcgcagcgta ttgatccgcg ttggaagtag aaatcgctct gcgtttcagc aacttgccaa
     3601 aaatcgatgc catggggtta gtgtagcaaa cgccaccgcc ccgaaacccg ctgccgaacg
     3661 ctaaagcggc agccgccagc cgtcgattac tgcatcggcg caatcggcgg ccgacatcgg
     3721 tgcggcaaac aagtagccct gcatttcgtc gcagtcgttg gcgcgcagaa agtcgagctg
     3781 tgcctgggat tcgacgccct cggccacgac cttgagtcgc aagctgtggg cgaggttgat
     3841 gatggccttg gcgatcgtgg cgtcgccctg gtcagtggtg atgtgcgaaa tgaacgcgcg
     3901 gtcgattttg agcgcatcga ggggaaatcg cttcagatag gcaagactcg aatagccggt
     3961 tccgaaatca tcgacggaca aacgtacgcc gtacttcttc atgttgtcca gcacctgaac
     4021 cgcatccctc gagtcgctca tcagcaccga ttcggtcaat tcaaattcca gcaaattcgg
     4081 gttgatttgg aattcatgca gcagcttgcc gatcgtggag tcgagattgt tacgcgcgaa
     4141 ttggcgcgcc gacagattga ccgcgatcgg gcaatctctg agtccaccga cctgccatgt
     4201 gcggatctgt tggcacgcct gccgcatgac ccaatcgccc acttcattga tgagcccggt
     4261 ttcttcgaga accggaatga attcgttggg cggcaccagt ccgcgtttcg gatgattcca
     4321 gcgcaacagc gcttcgaagc cgctgatccg ccctgtgttg atgtcggctt tgggctgata
     4381 gtgcagcacg aattcggtgc gggcgagtgc gccgcgcaat tcggtctcga tctgcaaacg
     4441 ctccgccgcg cgcgcattca tttcgggcag aaaaaatcga taggtgttgc ggcctgcttg
     4501 cttggcccga tacatcgcca tgtcggcatt cttgagcagg gcatcgggat tcgaaccgtc
     4561 gccgggcgca atactgatgc cgaggctcgc tgtgacatat acctcgtgac catggacgtg
     4621 aaacggtttt gcgagcgtcg caatcacgcg ttccgccacc attgtggcgt cctcgggcac
     4681 gatcaagtgg tcgagcacga gcgcgaactc gtcgccgctc aagcgggcga ccgtgtcggc
     4741 cggtcgcagg catttcttga gacgcacggc gatttgcgtg agcacctcgt cgccgccgct
     4801 gtgcccgagc ttgtcgttga tcgatttgaa atgatcgacg tcgaagaata tgactccgac
     4861 gggccgtttg tcgcggtcgg tctggcgcag cgtttgccat agccgatcga gatataaatt
     4921 gcgattcggc aggccggtca gcgcgtcgaa ttgcgcgagg tacgccaagc gatcctcggc
     4981 gcagcggcgg tcgatggccg cggcaagcgt gttcttgatc ccttgtagaa aatccacgct
     5041 gtcttgcgcg aacgcgccgg gggagcgcga atagactccc agcagcccat aaggaccgtt
     5101 gacgccccac accagcactt cggcggcgct gcggatcctg tggtctgaaa tgatgggcga
     5161 gggcgggtaa cgagctcctt tcggaaagtc ttcgaccaaa atcgcctcac gggcgccgat
     5221 cgagaagcga tcttcggttt cggtcacggc gtcgaagttg atttgttccg cccaggctcc
     5281 ctcccagccc gtgcccgcct tgaattgcag catcttctcg tctgcagata aggacagata
     5341 gcggctgaat tgcggttgca ggcccttggt gatggttcga tccgcctcga tgagcagttc
     5401 ctcgaattcc gggttttgca gcgcgaattg gccgaatgcc gcaatcagac tctgccgcat
     5461 ggtttggcgc tgaactgcct gggtcgcgag cagttgctcg gtgatgtcgg tgacggtacc
     5521 gccgattcca ataatccgtc cgtcttggtc gtgaatcgga aacttcgaga ttaggttcgt
     5581 ccggcatcgt tcgttgcaag gacgctgctg ctgaaactct gtcttcttgc cgtgcatcaa
     5641 caccggcgca tcgtggattt gcaatgcccg ggccagctcc ggggcgaaaa tctgcgcatc
     5701 ggtccgtccc agcacaacat ggcgcgccaa accgaattca ttgacaaact ggtcgttgac
     5761 gtgaagatag cggccggcgg cgtccttgat gaacgtcgct gccggcgcat ttttcaaaaa
     5821 tgcctgcagg tgcgcttcgc tgtgttcaag gcgccgagcc tggagcgccg caccactgag
     5881 gtcgcgcgat gcgcctgcga agcccaagag ttcgccgcga tggttgagct gtgccgtcaa
     5941 tacgacatgc gcccaaaagc gcgttccgtc tttgcgccgg caccagactt tttgatcggt
     6001 cctttcgagc gacgcgctgt cgagcagctg ctgcgcccgt ccgcgctccg tgtccgagtt
     6061 gtcgaacagc tgcgcgaaat cccggccgac gatctcctgc gggatatagc cgtacagcgc
     6121 ttcgcccccc gcgttccaac tggtcactcg tcccgccggg tcgaggatta ccatggcgtc
     6181 gtccacgccg ctgcccatga cgggggtcgc cgcagcaata tgttgattgg gtggtattgc
     6241 gtgccgctga gcggaggccg cgtcagtcat acgcaacctg agctgcaaga aagatcgttc
     6301 gaaggtcttg atctgttacc catgatgcac acgcgcctgc gaccgcttgc aacatcggtg
     6361 acgccccgag gttagccgat cggcctgccc gagattatcg gaataagtgg ctcaaactga
     6421 aggtaaaaac cccctaatcg cctagggtct ttaccttaag tcgccatcgc tgataaagac
     6481 gtgacgaggg tctcattggg tgcttttgtg atgctgcgat gcggccggac tgatgcggcg
     6541 ttttcggaca ctcggcgtgc cctaggccac actgcaattg gcggcgagca tttcacgctt
     6601 cagccagccg taccattgcg gcagcccctc gccgttgtag gcggaaacgc gaaaaacgcc
     6661 aagattcgga ttgacgctaa gcgcgttcgc aaccgcccgc tcgacgtcga agcggatgtg
     6721 cggcagcaaa tcggtcttgc tcagcagcag cacctcggcg gcggcgaaca tgtgcggata
     6781 cttgagcggc ttgtcttctc cctcggtgac cgagaagatc acgaccttcg catgctctcc
     6841 caagtcgaac agcgcagggc acaccaagtt gccgacattt tctatcataa ggagcgacga
     6901 cgatgccggc tgcagcgcat cgagagcgcg ctccaacatg tccgcctcga ggtggcagcc
     6961 tgtgccggtg ttgatttgca cggcggctac tccggtggca cgaatgcgct cggcgtcgtt
     7021 cgatgtcgct tgatcacctt cgatgacgct gatccgtacc tgagatttaa gatccgtcag
     7081 cgtgcgttcg agcaaggtgg tcttgccggc acccggactg ctcaccaagt tcagcgcgag
     7141 aatgctgttg agggacagcc gctttctgtt gcgtgcggca atcagctgat tcttgctgag
     7201 tatctgggct tccagctgga tcgtgtgtac gtctcgtccc ggcacggata ccgcaatctc
     7261 gtgggagtga accggcagtt gctggcgaaa cggcacggcc gcaaattctg gcatgtgagc
     7321 gtgcacgtgg gtgtgtcgcg tgccgtccgc atgaacgtgc tcgtgttcca ccccgtgcag
     7381 cgtgagtgtg gcttcatcag tgttgccgca gccgcaagtc acgcacatga ttgcacctcg
     7441 atctctttga tgttgagttc ctgcccggcg atcaatctca agctcgaggt gccgcagcgc
     7501 ggacagcggc cgacgagtgt ctccaacggg acttcggcac cgcactcgtt gcagcaccca
     7561 agaccgggaa tttcgacgat ttgcagctcg gcacccgcga gcggtgtgtc cgcggcgcat
     7621 atatcgaagc aaaagcgaat cgcgtccggg aggatggccg acagactgcc tatttcgagg
     7681 cgaacgcggg tcacgcgttg acctaccgcc tgctcggaga cgattgcgac gacgctgcgg
     7741 gtgatcgaga gttcatgcat gttggcagtc aacaaatacg cggcagttgt tcgccgacca
     7801 gcatatcgac cacgcggtca ccgccgatcg cggtcgacag gatcacgctg ccggcgggtt
     7861 gcgcgcagac ctcgccgatg agggcggcat cgcgtccggc cggatggctg cgcatcgccg
     7921 cgaccacggc agcggcgtgc gtccccggaa caatggccac gagttttccc tcgttcgcga
     7981 ggtacaaggg gtcgagaccc aagagctcgc acgcgccgcg cacggtctcg ttcagcggca
     8041 gcgcagcctc ccgcaatcga atgccgacgt tcgagcttga tgcgaactcg ttgagtactg
     8101 tcgcaacacc gccgcgggtg gcatcgcgca ggcagtgaat atttggacat accgcgagca
     8161 tggcgctgat gaggccatac agaggctgac agtcgctgat cacctcggcg gccagcgcga
     8221 gctcgttacg cgccaacaga atcgcaactc catggtcgcc gaggttaccg ttcacgatga
     8281 cggcgtcgcc cggctgcgcc cgggtcgcag cgatgtcgac acccgccggc acgacgccga
     8341 ttccggccgt gttgatgaac acaccatcgg cggcaccgcg ctccaccacc ttggtgtcgc
     8401 cggtgatgat ggctacgccg gcagcgcgcg ccgcactgcc catgctgcag gcgattttcg
     8461 tcaagtcggc gacgctcagg ccttcttcca aaaccaaccc gcaggaaagg aacaacggcc
     8521 gcgcgccgct caccgccaaa tcgttcaccg tgccgcaaac ggcgagcttg ccgatgtctc
     8581 cgccgggaaa ctcaaggggg gtcaccacgt aggtatcggt ggtgaaggtc aagcggtcgc
     8641 cgagcgccgt gagttccgcc agcggaatgc gtgcctggtc ctccagggtt gcgagcagtg
     8701 gattgtaaaa agccggcaac aggatcttct cgatcaatcc gcgcatggcg cgtccgccgc
     8761 cgccgtgcgc catggtcacg cagggcgcga ccggcaccgt tgccggcatc gcaatcgagg
     8821 atagcgacgc atcgtagcgc gcattcatgt cagcgcgccc gccgtgagac gccggtcttt
     8881 cacatcgatg cggccatact gatagtaggc ggcgcaggcg ccctccgagg agaccatcaa
     8941 cgcgcccaag ggtttctccg gcgtgcaagc ggtaccaaac accttgcatt gctgcggttt
     9001 gatgacgcct ttcagcacct cgccgcattg gcaggatttg gggtccgcga tcttcagatt
     9061 gggcacggcg aatttgcgct ccgcatcgaa ggcggcgaac tcgccgcgca tgcgcacgcc
     9121 cgagtggtcg atggagccga ggccgcgcca ttcgaagaac tcgcgcaatt cgaagacttc
     9181 ggtgacggcg gcaagggcac tgacattgcc gtcctgcggc acgatccgtg tgtactgatt
     9241 ctcgacttcg cagcggccct cgctgatttg cttgatcacc atccagagcg attgcaagac
     9301 gtcgagcggc tcgaagcctg ccaccacgat gggccggcga tagtggctgg caatgaactc
     9361 gtagggccgg ttgccgatga ccatgctgac gtgaccgggg cccaagaagc cgtcgaggcg
     9421 caaatccggc gaatcgagaa tcgccttgat cgtcgggatg atggtgatgt gattacagaa
     9481 caacgagaaa ttggcgatgc cctcgcgccg cgcctgcaat acggtgagcg cagtgctcgg
     9541 catcgtggtc tcgaagccca acgcgaagaa aacgacttcg cgctgtgggt tcgcgtgggc
     9601 aatgtgcagc gcatcgagcg gcgaatacac catgcgtacg tcggcgccgt tcgccttggc
     9661 gtgcagcaga ctgccttgcg aaccgggtac ccgcatggca tcgccgaagg ttgtaaatat
     9721 gactttcggg ttgcgggcga gcgccacgca atcgtcgacg cggcccatcg gcagcacgca
     9781 aaccgggcaa ccgggtccgt gcacgaattc gatactgtcg tcgggcaaca tggccttcag
     9841 gccatagcgg aaaatggtgt gcgtatgtcc accgcacacc tccatcactt gcaggggccg
     9901 gcggcgtgcc gccgcgagcg gaaccgccag gcgcgcgatt tcggcgacca aggtgcgggc
     9961 cttttcggga tcgcgaaatt cgtcggcgaa acgcatgctc aaagttccga cgccgccgaa
    10021 ccgcgggtgt cggcgagcag agtgtgcgtg agatcccaca ggatgtggta gatcgcgaca
    10081 tgagtttctt gcacgcgatg aatactgtcg ctcggcgcca ccaggcaatg atcgagagcc
    10141 gcggagctga gcattttgcc gccatcgccg ccggcgagcc cgatggtggt tgcgcccaat
    10201 tgcttggctg cggcgaacgc cagcaataag ttgtcggaat tgcccgaggt cgagacgccg
    10261 atgagcacgt cttgcggacg cagcagcgca atgacttggc gcagaaaaac gtgctggaat
    10321 cccaagtcgt tgccgaccgc ggtcagcgtg gcgcaatcat tcgccagatt gtgtgcggcc
    10381 aaagcgggcc ttccggcggt gaccggatgc tgaaattcaa ccgcaatatg tgcggaatcg
    10441 cagctcgagc cgccgttgcc catggtgtac agccggccgc cgcgggtata cgccgcagcc
    10501 agcgctcgcg ccgccgcgac cacttgcggg gcgaactcgc ggaagaatcg ctgcttcact
    10561 gcgaggcttt cgtccgattt ttgccgcacc gattccagca gcacttgctg catttgctcc
    10621 tcgtcgtgcc gctgcgccgc caggaaggga tacagtccgg caagttgcga attctgcagc
    10681 gggctgctca tgctagttcg accgccgcat cgcttcgatc tcgctttgcg cctcgccgag
    10741 ctgccgcagc agatccagcg tgagttgcgc ttcttgttcg tcgattcggc tcatcgcaaa
    10801 cccaacatgc accaacaccc agtcgcccac gcaggactcc accgagtgca attcattgac
    10861 gacgcaggcg aggttgatct ggcggcgcac gccgcacaca tccaccattc ccagtttatt
    10921 gccggcgtcg gtgatggcga cgatgcggcc cggaatcccc agacacattg aactctcctc
    10981 taatgcggcc gcgccgcggt ttgcagcagg cgcgccgccg cgattaccgc ctgccccagc
    11041 gccaagccgc cgtcgttgca cggcacttga tcgtgggtga gtacatgaaa tcccttcgct
    11101 tcgagacccg cgcacacgcg ctcgaacaga acccggtttt ggaatacccc gcccgagagg
    11161 gccaccggcg gcagcggcgc gacgtcgatc cgagcggcga ctttttcgac catgcgcacg
    11221 atgctcgagg cgaggccgcg gtgaaagcgc gctgccatca ctccgaccgg cgtgtgcagg
    11281 atcaagtcgc cgaacaatgc cgcccacatt cccaagggtt cgatgtacgg aagcccggcc
    11341 atccgcggga tggggaacgg ataactcaac cggtcgtcct cggtgtcaag gcattgccga
    11401 tcaacggcag cctccatttc cacggcgcct tggccctcat accaagcgcg ctcgcgggcg
    11461 aggcccattg ccgcggcggc tgcatcgaac agccgcccgc aggaactcgc caacggcgaa
    11521 ttcaagccgc gcttgagcat gccgtcgagc aacgcccgcg gctgctgctc gagaaagcga
    11581 tacagctcaa gatccgcgta gttcatggca aacgtcgccc aacccatctg cgcttggata
    11641 tgagcgtagg tgttgcgcca aggttcacga acggcggctt cgccgccgag caatgcaacc
    11701 cgcttgaagg ttccgaggcg ctggtagccg gcataatcgg ccagcaagaa ctctccgccc
    11761 cagagactgc catcctcgcc gtagcccatg ccgtcgagcg ccacgccaat gaccggcgcc
    11821 gcagcgcgcg gtacaccgtt atcggccaga cacgctgcga tgtgcgcgtg atggtgttga
    11881 ctcgcgagca gccgcaaatt gccgtcgtcg gcgtgacctc gggcaatttg cgtcgaggaa
    11941 tagtcgggat gtaaatcgca cgccagtgcc tcgggcgcaa atgcaaaaaa tcgttggtag
    12001 tcggcgagcg cacgtcgata gtcggcgcgc gtgagcgggt cctgcagatc gcccaagtgc
    12061 gcggagacga tggcggcgcc gtcacgcagc atgcagaagg tattcttgag ctcaccgccg
    12121 aagccgagca ccgcgggcgc tgcctcgaag cccggcggca acgcgagttc gagtggggcg
    12181 aatccgcgcg cgcggcgcag cactcggaca cgcccggcga ccacgcgcgc caccgaatcg
    12241 tcgacgcggc gcgcgatggg acggttgtgt tcgaggaagt agtcggcgag cccactcagc
    12301 cgctgccgcg catccacggc atcgatcgat tgaggctcct cgctcaggtt gccgctcgtc
    12361 aagacaatgg gacgctgcat gcgtttcagc agcaagtgat gcaggggcgt gttggggagc
    12421 atgaatcccc aggtatcaag gcccggtgcg acgctgccgg caatctcctg cgtcgcattg
    12481 gcgacgcgcg tcagcagcac gatcggagca actgatccgg caagcagaac ggcttccgat
    12541 tcactcacta gcgcaaagcg gcgaatcacc gccaaatcgc gcgccatcag tgcgaacggc
    12601 ttgagttcgc gctgtttcag ccggcgcagc cgcgcgacga cgtcgggttg tgtggcatcg
    12661 caggcgagtt ggtaaccgcc caagccttga atcgcgagaa tgctcccccg ctgcagcaag
    12721 gtgcaggccg catccgcctc atccaaggcc gacagggctt ccagagtcat gacgtgcccg
    12781 tcggctcgcg ccaaggtcag tttcggaccg caggcgtgac aggcgatcgg ttgtgcgtga
    12841 aaacgccggt cctgcggatc gtggtattcc gctgcacagg cggcgcacat ggcgaatccg
    12901 cccatggtgg tgttggcgcg gtcgtagggc atgtgtcgca cgatgctcaa acgaggaccg
    12961 caatgggtgc aattggtgaa cggatagcga tagcgtcgcg caaaggggtc gaagatttct
    13021 cgcacgcatt cggcacatga ggcggcgtcc ggcggcacgc cggtgctgat cgcagtaatc
    13081 tcgctggcgg cgatgaagaa tcccgcgtcc gacggcaagg ccgtcgcggg ctcgcgttcg
    13141 attcgatcga tgcgcgccag cggcggcgac tcgcccggca agttgagcac gaattcttcg
    13201 acagcggccg ccggaccaca gacgtgcatg cgaacgccct ggccatcgtt cgccacccag
    13261 ccgcgcagtc cgtgccgctg ggcgaggcgc cacatgctcg ggcggaagcc gacgccctga
    13321 accaagccgc gtactcggat gatttctgcc gtctcgctca tctcgcgagc tcccgtgcca
    13381 gcggccagag caacacgcga ttgttgccgg aatccgcgat gacacccatc ctttgctgcg
    13441 cgttgatgcc gtacggccac gataggctgc accgagtcgg cgactgccag gcattgtcgc
    13501 ctttggcgtt gaactgcaac tgccccgtga gcgcgcgcgc gcggctgccg ttcttgcaat
    13561 cctcgatatg ccaggcaatc aaccgcgagt tggccgtgtc cgcacagatg caccaggggc
    13621 cggcggccgt gatcccgtag ggcatgtgca gagtggccgc actcggccag tacagcgatt
    13681 ggttgtgatc caccaggtca aaatcgttct gtcccagcac ccaatcggca ggcgcgttgc
    13741 cgacggtcgg cggcgagtcc cacacgagga tgcggttatt gccggcatcg gtcacgcaga
    13801 gttttccgct ccatgtcgtg atggcatgcg gccagcgcat gctgccggca gatggcgcgc
    13861 cgccgccgtt ctcgtcgcgg cattcgaagc tcgtctgtcc gagaaccagg tctgcggccc
    13921 ggccgttctg ctgcggcagc ccacgccact tcaagacgcg tcgattgccg gtatccgcca
    13981 cccacagatc ggcaccatcc caatgcacgc catacggcca gaacaggctg tccgccgacg
    14041 ccccggaggc gccgcgattg gattccgtgg cagcgaaatc cgcctgcccg agcaccacat
    14101 cggccgggat gttgtcggcg gtagggcatc ttcgccagat gagcacacgg tgattccatg
    14161 catccgcgac cgcgaagccc tcgttcactg cgcatatgcc cgtcggcaca ttcagcgtaa
    14221 tcgcactcgg cgcacccttg gcattgcggc cctcgcgcga gaagtcgggc tgaccgatca
    14281 cccaatcggc gggcacaccg tcagcgcagg ggatctgcgc ccagccgagc agacgatgat
    14341 ggccggtatc gctgatccag agcggtcccc gctctcccat gagacctgcg ccgcgcggcc
    14401 cgaaaagcgt gaacgcgtcg ggcttcaccg gaattgcgag attatcctcg ccccgcgctc
    14461 cgccgatgca aactcgcgga ccctcggtgt cgagcaccg
//
LOCUS       DASWZT010000217         9705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_64524, whole genome shotgun sequence.
ACCESSION   DASWZT010000217 DASWZT010000000
VERSION     DASWZT010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9705
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64524"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VGE92_05970"
     CDS             <1..232
                     /locus_tag="VGE92_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313399.1"
                     /translation="AFAAAGGGALEVVTGANGAQHADANAVLALKHGLQGSVGSDFHN
                     PAHIWNPLGRSLKLPDCVTPVWRNYITQAATP"
     gene            229..453
                     /locus_tag="VGE92_05975"
     CDS             229..453
                     /locus_tag="VGE92_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEY1313400.1"
                     /translation="MSDEDSNVNTDLFKNIERLRQLRIEHRDLDDVISRLSMDFKVDE
                     LQMKRLKRRKLMLKDQIARLESQLIPDLNA"
     gene            450..1757
                     /locus_tag="VGE92_05980"
     CDS             450..1757
                     /locus_tag="VGE92_05980"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY1313401.1"
                     /translation="MNELVQRIAAFFDILSTRNVLIEIGAVLLCLLAGWLVGAVLSNR
                     YRRRRIKAPMALTWSYFTSQGGVVALPAILALLLVVLARNLLQTHFDVTVLDAAAHLI
                     GAYVAVRVVVLLFAASLGNKSWMHHWEGRLTLVLWLAIAAEYLGWLDPIIATLDSIGM
                     AAGKSRISLWSVLKLLFTLTVFVLVAAWVSRWVERRLKKLSNLAPSTRIGISKFANAF
                     LIGLSILMGLNAAGVDLTALTVLTGAIGLGLGFGLQSIAANFVSGFVLLMDRSIKPGD
                     VISLSGQSGTSTENFGWVQELRGRYVVVRDRDGVEMLVPNQQLISNAVINWSYTDPRI
                     RLKLPIRVSYRDDPELALAILLTACEGQSRVLREPAPVSRLMHFSDSGIELELRFWIS
                     DPQEGVNNVRSEVNRAIWRLFKEHRITIPVAQREIVVTNSPWP"
     gene            1748..2170
                     /gene="arfB"
                     /locus_tag="VGE92_05985"
     CDS             1748..2170
                     /gene="arfB"
                     /locus_tag="VGE92_05985"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF012684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEY1313402.1"
                     /translation="MALIITPDIVISDEELEWKFIRSSGPGGQNVNKVASAAQLRFLL
                     PQNVSLPVSARNRLRRLAGQKLIDDGSILLKSMSERSQEANRRAALERLEAMIRAALI
                     EPKIRKKTRPTKASKERRIDSKKRRGATKQQRGAHGWD"
     gene            complement(2167..4686)
                     /gene="plsB"
                     /locus_tag="VGE92_05990"
     CDS             complement(2167..4686)
                     /gene="plsB"
                     /locus_tag="VGE92_05990"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF003441.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HEY1313403.1"
                     /translation="MNPVAGLRRAWNWSVRKILSLWVRVTIKPDDAAAAIAASPRPVC
                     YVLERESHTDSAVLSLVCGKLGLPRPERRLLIGTRRADRAYFELIRPAGFWSTRQAVR
                     APRYLVSLIAAAAAHPLLDVDLVPVAIFWGRAPHKEASLWRLLFAENWVLVGRFRKAL
                     NVLVNGRNALVHFGDAVRLRDALQDGMNEQRSVRWVLRTLRSSLRAQRASTIGPDLSH
                     RRTLVTQLLKTADVRSAVRREMQSRAAPRRGGSPRRAALLAARKYAVEIAANYSQPFV
                     RFMSVLLGWLWNRLYDGVDFEHVENLNEVGDGAEIIYVPCHRSHMDYLLLSYVIYRKG
                     FAVPHVAAGINLNLPVIGRFLRKGGAFFLRRTFKGDALYATVFAKYLGIMMARGHSLE
                     YFIEGGRSRTGRLLPPRTGMLSMTVRAFLHDPKRPVVFMPVYFGYERIVEGSTYIGEL
                     SGAPKEKESVIGLLKAVVSVLRSKLGKVHVNLGQPIALDELLCRHNPDWRSAAADCAE
                     SRPAWVNEAIGDLATRINIEINAAAAVTPINLVAMAILPTPRQALPEVDLVRQVEVYQ
                     QLLRAAPYGPWVTVTADSGAQMIRYAEAFGLLERQAHALGDIMRMKAENAVLATYYRN
                     NILHLFAMPSLLACCFVSNASMRTSDIRRLVWRVYPYIAAELFLRWDEGSVEAVADTL
                     LDALAGLNLLSANADRSEWQRPASTSIEAIRLSMLAQATIQTLERYYLAIALLLQAGS
                     GSITQEALEQLCHLMAQRMSVLYGLNSPEFFDKSLFRNFIDLLRRRNVIQVSADNRLL
                     FGDPLLGVAADAQLVLSEQIRHSILQVTLGQEAATDTVQGV"
     gene            4890..5777
                     /locus_tag="VGE92_05995"
     CDS             4890..5777
                     /locus_tag="VGE92_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313404.1"
                     /translation="MFRIALLMLMATSPFAAKAVDIETAAKIYQDADIREQVRASLGS
                     MPAHIRQLFSADTSAQLSDQQLELVTAAAKRGFRIDVFEAPALSALATNLDPETVKKT
                     DAFLMSSVGRRMVAADTATATLEEAKIDSIMNGKLTAPSTPRRDALFEKLEAASRSTE
                     STVQIFLGMGQAVAVGTAVGSGRDPADVAESARKSGEASRQAMEQNMREPLRRYMAYS
                     YRDLSDTDLKHVLQFLESPPGKQYVSAYIASLGAGFDAMGKRCGEQLGESLRELAQAQ
                     LDVPTVGAPPSIAAPAHTQ"
     gene            complement(5783..6274)
                     /locus_tag="VGE92_06000"
     CDS             complement(5783..6274)
                     /locus_tag="VGE92_06000"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY1313405.1"
                     /translation="MTTVVFSHGKESGPWGAKITAMAALARELAMAVESVDYRGMDDP
                     GARVAKLAATGAVLEAPLVLVGSSMGGHVSAAAASRLKPKGLFLLAPAFYMAGYEEYT
                     PQDVSCPTAIVHGWHDDIVPVENSIRWAREHQAALHVLNSDHRLEDQIGAICRLLRDF
                     LRN"
     gene            complement(6271..7872)
                     /locus_tag="VGE92_06005"
     CDS             complement(6271..7872)
                     /locus_tag="VGE92_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313406.1"
                     /translation="MTPSNPGIQVEEERGPDQTISRLGTARTAFVGRTLRGPVDRPIL
                     VSSFADFQHVFGGLWQPSPLGYAVEQFFDNGGREALIVRVVNGARPATLSLSAGTGTL
                     CLQAVRPGTREFLRASVDYDNVAAQNTSVFNLTVQRVRTQGTAQVEDQEIFRRLSLEP
                     ASANYVPRALCGSELVQVAGDLPAQRPDRTLDAVSGQASGYVNSNSDGDDGAPLTDYD
                     LIGRAGDRTGLFALASAEYFNFLCLPPLSRDQDVGPIALLVGARFCKERRALLIVDPP
                     AHWHTADDALRGMREWNFSDENALMYFPRVLAHDKLRGHFESFAPCGAVAGMLARGDD
                     ASAVWQPTKSDEAILRPGYRPTCLVSEDRRIRLAALGVNTILAGRSAARIGVAPRTLA
                     GGKATNADWQYLGARRLALFIVNSIERGTRWVAMVRPHLETAELVAAQVRAFMEGLYE
                     EQAFGARRMQDAFFVVCDQRSEFHLVIGFAGRATHGFHSFRIVHAASGSRVAPVSLNR
                     LNTANYSPAELEWVEGLARSLSDEI"
     gene            complement(7885..8571)
                     /locus_tag="VGE92_06010"
     CDS             complement(7885..8571)
                     /locus_tag="VGE92_06010"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY1313407.1"
                     /translation="MIPLKDDSPAALKPWVTITLIAGCSCVFLWQRSLDTVSSRRALA
                     AFGAIPAVLLTAARLPADLQWIPRYATPFTAMFLHGGWMHLLGNMLFLWIYGDNVEDA
                     MGHPRYFLFYILCGVAAVFVQALSNPNSPYPIIGASGAISGVLGAYLLLFPRSRVLTL
                     VLLPFFFTTLRIPAILLLLLWFAVQLISDLQVSDGGASVAFRAHIGGFLCGILLVPVM
                     KRRDVALFAL"
     gene            complement(8568..8714)
                     /locus_tag="VGE92_06015"
     CDS             complement(8568..8714)
                     /locus_tag="VGE92_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313408.1"
                     /translation="MSSEWLKVMLEEIARKKAEAEQARLEEQARRDAPVQSGAPPRKT
                     PERE"
     gene            complement(8711..>9705)
                     /locus_tag="VGE92_06020"
     CDS             complement(8711..>9705)
                     /locus_tag="VGE92_06020"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leukotriene A4 hydrolase C-terminal
                     domain-containing protein"
                     /protein_id="HEY1313409.1"
                     /translation="ITPTVIAGDKSLTSVIAHELAHSWSGNLVTNATWRDVWLNEGFT
                     NYLESRILTQVYGEQRAAMEAVLGLQALRKDLARLKPQEQALLIDLRDRDPSLVFSTV
                     PYQKGRLFLNYLDAKFGRERFDVFLRGYFDHFAFKSITTEQFTAYLTENLLDRFPGIV
                     SHDQVLAWETAPGIPADAVLPVSSAFQPVDEARGAWLGGTLEAKKFGHDWVSQQWQYF
                     LNNMPAELSAKQLGTLDQAYGFTHSQNDEIEHSWLELVIHNNYQPGFARLEEYLKTIG
                     RGKLIAPLYTDLMKTPSGTEFAKRVFTKARPGYHPDTQTEIDAIVNPGAVEEAQ"
BASE COUNT         1895 a         3114 c         2993 g         1703 t
ORIGIN      
        1 cgctttcgcg gccgccggcg gcggcgcgct cgaagtcgtc accggcgcga acggcgcgca
       61 gcacgccgac gcgaacgcgg tgctcgcgct gaagcacggt ctgcaggggt cggtgggctc
      121 ggatttccac aatcccgcac atatttggaa tcctctgggc cgctccctta agctaccgga
      181 ttgcgtcacc ccggtgtggc gcaactacat aactcaagca gcgacgccgt gagcgacgaa
      241 gatagcaacg tcaataccga tttattcaag aacatcgagc ggctgcgcca gctgcgaatc
      301 gagcaccggg atctcgacga cgtcatttcg cgcctcagta tggatttcaa agtcgatgag
      361 ctgcaaatga aacgtctcaa gcgccgcaag ctgatgctca aggaccaaat cgcacgcttg
      421 gagagccagc tcattccgga cctcaacgca tgaacgagtt agtgcagcgg atcgccgcgt
      481 tcttcgacat tttatcgacg cgcaatgttc tgatcgagat cggcgccgtg ctgttgtgtt
      541 tgctcgccgg ctggttggtc ggcgccgtgc tcagcaatcg ctatcggcgg cgccggatca
      601 aggcgccgat ggccttgacc tggtcatatt ttacctcgca aggcggcgtg gtcgcgttgc
      661 ccgccatcct cgcactgctg ctggtggttc tggcgcgcaa ccttttgcag acgcacttcg
      721 acgtcacggt gctcgacgcc gctgcgcacc tgatcggcgc gtacgtcgcg gtacgcgtgg
      781 tggtgctgct gtttgccgcg agcctcggca acaagtcgtg gatgcaccat tgggagggcc
      841 gtctgacctt ggtcttgtgg ctcgcgatcg ccgccgagta cttgggctgg ctcgacccca
      901 tcatcgcgac gctcgacagc atcggcatgg cggccggcaa gagccgcatc agcctctggt
      961 cggtgctgaa gctgttgttc acgctgaccg tgttcgtgct ggtggcggct tgggtgagcc
     1021 gctgggtcga gcggcgcttg aagaaactca gcaatctggc gccctcgacg cgcatcggga
     1081 tttcaaaatt cgccaacgct ttcttgatcg gcttgagcat cctgatgggc ctgaatgcgg
     1141 ccggtgtcga cctgacagcg ctcaccgtgc tgaccggcgc catcggctta ggtctcggat
     1201 tcggcctgca gtcgatcgcg gcaaacttcg tcagcggctt cgtactgctc atggaccgct
     1261 ccatcaaacc gggcgatgtc atcagcctct cggggcagag tggcaccagc accgaaaatt
     1321 tcggctgggt gcaggaactg cggggtcgtt acgtggtggt gcgcgatcgc gacggagtcg
     1381 aaatgctggt gccgaatcag cagctcattt ccaatgcggt gatcaattgg agctataccg
     1441 acccgcgaat tcgactcaaa ttgccgatcc gcgtgagtta tcgcgacgac ccggaattgg
     1501 cgctcgcaat attgctcacc gcctgcgaag ggcagagccg ggtgctgcgc gaaccggcgc
     1561 cggtctcgcg cctcatgcac ttcagcgact caggcatcga actcgaactg cgcttttgga
     1621 tttcggatcc gcaggaaggc gtcaacaatg tgcgctccga ggtgaatcgc gccatctggc
     1681 gcttgttcaa agagcacagg atcacgattc cggtcgcgca gcgcgagatc gtggtgacca
     1741 attcgccatg gccctgatca tcaccccgga catcgtcatt tccgacgaag aattggagtg
     1801 gaaattcatc cgctcctccg gccccggagg gcagaacgtc aacaaggtgg cgagcgctgc
     1861 gcagttgcgt ttcctgctgc cgcagaatgt ttctctgccg gtgtcggcgc gaaaccgcct
     1921 gcgccggctc gccggccaga agctcatcga cgacggcagc atcctgctca aatccatgag
     1981 cgagcgcagc caggaagcca atcgccgtgc cgcgctcgag cgcctcgagg cgatgattcg
     2041 cgcggcgctg atcgagccca agattcgcaa gaagacgcgc ccgaccaagg cttccaagga
     2101 acggcgcatc gattcgaaga aacgccgcgg cgcgaccaag cagcagcgcg gcgcacacgg
     2161 ctgggactaa acgccctgga cggtgtcggt cgcagcctcc tggccgagcg tcacctgcag
     2221 gatgctgtgc ctgatctgtt cggagagcac gagctgggca tccgcggcga ccccgagcag
     2281 cggatcgccg aacagcagcc ggttgtcggc agacacttga atcacgttgc ggcggcgtaa
     2341 tagatcgatg aaattgcgaa acaggctctt gtcgaagaac tccggtgaat tgagcccata
     2401 cagcacggac atgcgctgcg ccatcagatg acacaactgc tcgagcgctt cctgagtgat
     2461 gctgccgctg ccggcctgca gcaacaaggc gatcgccagg tagtagcgct ccagcgtttg
     2521 aatcgtcgcc tgcgccagca tcgatagacg aatggcctcg atcgaggttg aagccggccg
     2581 ctgccactcg ctgcggtcgg cattggcact cagcaaattg agtcccgcca gcgcgtcgag
     2641 caaagtgtcg gcaaccgcct cgaccgaccc ttcgtcccag cgcaggaaca actcggcggc
     2701 gatgtacggg tagacgcgcc acaccaggcg gcggatatcg ctggtgcgca tgctggcgtt
     2761 gctcacgaag caacaggcca ggagcgaggg catggcaaac aaatgcaaga tgttgttgcg
     2821 ataataagtg gccagcaccg cgttctcggc cttcatgcgc atgatgtcgc cgagcgcatg
     2881 cgcttgtctc tcgagcaatc cgaaggcttc cgcgtagcgg atcatttgtg cgccgctgtc
     2941 cgccgtgacc gtcacccacg gcccgtacgg cgcggcgcgc agcagttgct gatacacctc
     3001 gacctgccgc acgaggtcga cctcgggaag cgcctggcgc ggtgtcggca agatcgccat
     3061 cgccaccaga ttgatcggcg tcacggccgc cgccgcattg atctcgatat tgatgcgcgt
     3121 ggcaagatcg ccgatcgcct cattgaccca ggcggggcgc gattcggcgc aatccgccgc
     3181 ggcgctgcgc cagtcgggat tgtgccggca caacaattca tcgagcgcaa tgggttggcc
     3241 gagattgacg tgcaccttgc cgagcttgct gcgcagcacc gaaacgaccg ctttgagcag
     3301 accgatcacg ctctcctttt ccttcggtgc gcccgacagc tcgccgatgt aggtactgcc
     3361 ttcgacgatg cgctcgtagc cgaagtacac cggcatgaac accaccggcc gtttcggatc
     3421 gtgcaagaag gcgcgcaccg tcatcgacag catgccggtg cgcggcggga gcagccggcc
     3481 ggtacggctg cggccaccct cgatgaaata ttcgagcgaa tggccgcgtg ccatcatgat
     3541 gcccaggtat ttggcgaata cggttgcata cagggcatcg cctttgaagg tgcggcgcag
     3601 aaagaaggcg ccgcccttgc gcaggaatcg tccaatcacc ggtagattga gattgatgcc
     3661 cgccgcgacg tgcggcaccg caaagccctt gcgataaatc acataggaga gcagcagata
     3721 atccatgtga ctgcgatggc aaggcacata gatgatctcg gcgccgtcgc cgacctcgtt
     3781 cagattctcc acgtgctcga aatccacgcc gtcgtacagc cgattccaca accagccgag
     3841 cagcaccgac atgaaacgca cgaagggttg gctgtagttg gcggcgattt ccaccgcgta
     3901 tttgcgggcc gcgagcaacg cggcgcggcg cggcgagccg ccgcggcgcg gcgcggcgcg
     3961 cgactgcatt tcgcggcgta cggcgctgcg cacatccgca gttttcaaga gctgcgtcac
     4021 cagtgtgcgg cggtgcgaca gatccggccc gatggtcgaa gcgcgttgcg cgcgcaggct
     4081 gctgcgtagg gtgcgtagca cccagcgcac gctgcgctgt tcgttcatgc cgtcctgcag
     4141 tgcatccctc aggcgcaccg catcgccgaa atgcaccagg gcattgcggc cgttgactaa
     4201 cacgttgagc gccttgcgaa agcgcccgac cagcacccag ttctcggcaa acagcaggcg
     4261 ccagaggctg gcttccttat gcggcgcacg cccccagaaa atggcgaccg gcacgagatc
     4321 gacgtcgagt agcggatgcg ccgccgcggc ggcaatgaga ctgaccaggt agcgcggcgc
     4381 gcgcacagcc tggcgcgtcg accagaaccc cgctgggcga atcagctcga aataggctcg
     4441 gtcggcgcgc cgcgtcccga tcagcaggcg tcgctcggga cgcggcaggc ccaacttccc
     4501 gcacaccagg ctcaagaccg cggagtcggt atgactctcg cgctcgagca cataacagac
     4561 cggccgcggg ctggccgcta tcgcggccgc ggcgtcatcc ggcttgatgg tcacccgcac
     4621 ccacagactc aagatcttgc ggacgctcca attccacgcc cgcctcaacc cagcaacggg
     4681 gttcatgatc aagccggtct aaggtgtggc gtcgccgcgc tcttgagtgt cgacggcctt
     4741 gcgtgtcgcg tcggtgttgg catcgatgaa caaaaccgac agtgacagtg tcaaaacccc
     4801 gaataagccc atgtgcacct cggaaatgcc ggccccgaca gcccggagtg tacaatcctt
     4861 ccggacttgc agtcactcgg agttcgtgca tgtttcgtat tgcattgctg atgttgatgg
     4921 caacgagccc gtttgcggcg aaggcggttg acatcgagac cgcggcaaaa atctatcagg
     4981 acgcggacat ccgcgagcaa gtgcgcgcct cgttgggttc gatgccggcg catatccgcc
     5041 agctgttctc agccgacaca tcggcgcagc tctccgatca gcagctcgaa ctggtaactg
     5101 cagccgccaa gcgcggcttt cgcatcgacg tcttcgaagc gcccgcattg agcgcgctcg
     5161 ccaccaacct cgaccccgag acggtaaaaa agaccgacgc cttcctgatg agcagcgtcg
     5221 gccgccgcat ggtcgccgcg gacactgcca cggccacgct cgaggaagcc aagatcgaca
     5281 gcatcatgaa cggcaagttg accgccccgt cgacgccgcg ccgcgacgcc ttgttcgaga
     5341 agttggaagc ggcctcgcga tccaccgaat caacggtgca gattttcctc ggcatgggtc
     5401 aggccgtggc cgtcggcacg gcggtcggct caggcagaga tccggcggac gtcgccgaga
     5461 gcgcgcgcaa gtccggcgag gccagccgcc aggcaatgga acaaaatatg cgcgagccgc
     5521 tgcgccgtta tatggcgtac agctatcgcg atttgagcga cacagatctc aagcacgtgc
     5581 tgcagtttct ggagtcgccc cccggcaaac aatatgtatc ggcctacatc gcctcgctcg
     5641 gtgccggatt cgatgccatg ggcaagcgct gcggcgagca actcggggaa agcctgcgcg
     5701 aattggccca ggcgcaactc gacgttccca cggtcggggc gccgccctcg attgcagccc
     5761 cggcgcacac tcagtgatga attcaattcc tcaagaagtc gcgcagcaaa cggcagatcg
     5821 ccccgatctg atcctcgagt cgatggtctg aattgagcac atgcagcgcg gcctgatgct
     5881 cgcgtgccca gcggatactg ttctcgaccg gcacgatgtc gtcgtgccag ccatgcacga
     5941 tcgcggtcgg gcagctcaca tcctgcggcg tgtactcctc atagcccgcc atgtagaaag
     6001 ccggcgccaa caagaacagg cctttgggct ttagccggct cgcagcggcg gctgaaacat
     6061 gtccacccat gctcgatccc accagcacca agggcgcctc gagcacagcg cccgtggcgg
     6121 cgagcttggc gacgcgcgca ccgggatcgt ccatgccgcg gtaatctacc gactccaccg
     6181 ccatggcgag ctcgcgagcc agcgccgcca tggccgtaat cttcgcgccc cacgggccgc
     6241 tctccttgcc atgcgagaac accacggtcg tcatatctcg tccgagaggc tgcgggcgag
     6301 accttccacc cattcgagtt ccgccggact gtagttcgcg gtattcagtc gattcaagct
     6361 taccggcgcc accctgctgc ccgatgccgc gtgcacgatg cgaaaactgt gaaacccgtg
     6421 ggtcgcgcgg ccggcgaagc cgatcaccag atgaaactcg ctgcgctgat cgcaaaccac
     6481 gaaaaaggcg tcctgcatgc gccgcgctcc gaacgcctgt tcctcgtaga ggccttccat
     6541 gaaagcacgc acttgcgccg ccaccaactc cgcggtttca agatgcgggc gcaccatcgc
     6601 cacccagcgc gtgccgcgtt cgatgctgtt gacgatgaac aacgccagcc ggcgtgcccc
     6661 caggtactgc caatcggcgt tggtcgcttt gccgccggcc agcgtacgcg gcgcgacgcc
     6721 gatgcgcgcg gcggaacgtc cggcaaggat ggtgttcacg ccaagcgcgg caagccgaat
     6781 gcggcggtct tcggaaacca ggcaagtcgg ccggtaaccg ggacgcagga tggcctcatc
     6841 gctcttcgtg ggctgccaca cggccgacgc gtcgtcgccg cgcgccaaca tcccggcgac
     6901 cgcaccgcac ggcgcgaaag actcgaaatg accgcgcagc ttgtcgtgcg ccagcactcg
     6961 cggaaagtac atcagcgcat tttcatcgga gaaattccat tcgcgcatgc cgcgcaaggc
     7021 gtcgtccgcg gtatgccagt gcgcgggcgg atcgacgatc aacagcgcac gccgctcctt
     7081 gcagaagcgg gctcccacca gcaacgcgat cggacccacg tcttgatcgc gcgacagagg
     7141 cggcaggcat aggaaattga agtattctgc gctggcgagc gcgaacaggc cggtgcgatc
     7201 gccggcacgg ccgatgaggt cgtagtcggt gagcggcgct ccatcgtcgc cgtcggaatt
     7261 cgaattgacg tagccgctcg cctggccgct gacggcatcc aaggttctat caggtcgctg
     7321 cgccgggaga tcccccgcca cttgcaccag ttccgaccca cagagggccc gcggcacgta
     7381 attcgcggac gcgggctcca gcgacagcct gcggaatatc tcctgatctt ccacctgagc
     7441 cgttccctgg gtgcgcacgc gctgcaccgt gagattgaac acgctggtgt tctgcgcggc
     7501 gacattgtcg tagtcgacgc tggcgcgcag gaattcgcgg gtgcccggcc gaaccgcctg
     7561 caaacacagc gttcctgtcc cggcgctcaa gctcaacgta gcgggacgcg ccccattgac
     7621 cacgcgcacg atgagggctt cacgcccgcc gttatcgaag aattgctcga cggcgtagcc
     7681 taatgggctc ggttgccaca gaccaccgaa cacatgctgg aaatcggcga agctcgagac
     7741 gagtatcgga cgatccacgg gtccgcgcag cgtgcgcccg acgaacgcgg tgcgcgcagt
     7801 gccgagccgc gaaattgttt gatcggggcc gcgttcctcc tcgacctgga ttcccggatt
     7861 gctcggcgtc acgggcggac tttatcacaa cgcgaataac gcgacatcgc ggcgcttcat
     7921 caccggcacg agcagtatgc cgcagagaaa tccgccgatg tgcgcgcgaa aagcaacgct
     7981 cgcgccgccg tcggatacct gcaggtcgct gatcaattgc acggcgaacc acaacaacaa
     8041 cagcaggatg gcgggaatgc gcaacgtggt gaagaaaaac ggcagcaaca ccagcgtcag
     8101 cacgcgggag cgcggaaaca acaataggta tgctcccagc acgccggaaa tcgcaccgct
     8161 cgcaccgata atggggtagg gcgaattcgg attggacagc gcctggacga acaccgcagc
     8221 cacgccgcac aagatataga acaagaagta acgcggatgc cccatggcgt cctcgacgtt
     8281 atcgccgtag atccacagga acaacatgtt gcccagcaaa tgcatccaac cgccgtgcag
     8341 gaacatcgcc gtgaacgggg tcgcgtagcg tggaatccac tgtaaatcgg ccggcaggcg
     8401 cgcggcggtg agcaataccg ccggaatcgc gccgaatgcg gccagtgcac gccggctcga
     8461 taccgtatcc aacgaacgct gccacaagaa cacgcagctg cagcccgcga tcaaggtgat
     8521 cgtgacccac ggcttcaatg cggcgggcga gtcatccttg agcgggatca ttctcgctca
     8581 ggcgtcttac gcggcggcgc accgctctgc accggcgcgt cgcgccgcgc ttgctcctcg
     8641 agccgcgctt gctctgcctc ggctttcttg cgcgcgatct cttcgagcat caccttcagc
     8701 cattcgctgc tcattgcgct tcttcgacgg cgccgggatt gacgatcgca tcgatctcgg
     8761 tctgagtatc cggatggtag ccgggacgcg ccttggtgaa cacgcgcttg gcgaattcgg
     8821 tgccggacgg cgtcttcatg aggtcggtat acaacggtgc aatcagtttg ccgcggccga
     8881 tggtcttgag gtactcctcg aggcgcgcaa aaccaggttg gtagttgttg tgaatgacca
     8941 gttcgagcca gctgtgttcg atctcgtcat tttggctatg cgtaaagccg taggcctgat
     9001 cgagggtgcc gagctgcttg gcgctcaatt cggcgggcat gttattcaaa aaatactgcc
     9061 actgctggct cacccaatcg tgcccgaact tcttggcctc gagcgtgccg ccgagccacg
     9121 cgccgcgcgc ctcatccacc ggttgaaagg cgctcgacac gggcaatacc gcgtcggccg
     9181 gaatgcccgg agcggtttcc caggcgagca cctggtcgtg gctgacgatg ccggggaaac
     9241 ggtccagcaa attctcggtg agatacgccg tgaactgctc ggtggtgatg ctcttgaacg
     9301 caaagtgatc gaaatagccg cgcaaaaaaa cgtcgaagcg ctcgcgcccg aatttcgcgt
     9361 cgaggtagtt caagaacaag cggcccttct ggtagggcac ggtgctgaag accagactcg
     9421 gatcgcggtc gcgcaggtca atgagcagtg cttgctcctg cggcttgagc cgggccagat
     9481 ccttgcgcaa tgcctgcaag cccagcaccg cctccattgc agcgcgctgt tcgccgtaga
     9541 cctgcgtcag gatgcggctc tccaaatagt tggtaaagcc ctcgttcagc cacacatcgc
     9601 gccaggttgc gttggtgacg agatttcccg accacgaatg tgccagctcg tgggcgatca
     9661 ccgaagtcaa gctcttgtcg ccggcaatca ccgtgggcgt gatga
//
LOCUS       DASWZT010000218         8176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_25827, whole genome shotgun sequence.
ACCESSION   DASWZT010000218 DASWZT010000000
VERSION     DASWZT010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8176
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25827"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VGE92_06025"
     CDS             complement(<1..552)
                     /locus_tag="VGE92_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313410.1"
                     /translation="MIEPLRRFSTADWIVAGTALFIIGNGASVCIGWWLRIPILVQLS
                     PDAPTHFNTALIFIVLGVGELGLVLRRRGIVMGVAVAVTCIAGAEFVEYALGLDLGID
                     RLFAVPFVGLDASYPGRMSGNTIACFLLVCAAQVLMSKPDPHAGAATTAAVILKTLAG
                     GIAFLAMLGYVVGLKSAYGWTDSV"
     gene            complement(733..1068)
                     /locus_tag="VGE92_06030"
     CDS             complement(733..1068)
                     /locus_tag="VGE92_06030"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEY1313411.1"
                     /translation="MTLSDELSRLAELRASGTLTEEEFARAKARLLNEQAGQTGQAAP
                     PNEPVVAAVNAFRRSRSDRIIGGVCGGMARATGVEAWVWRLLFAIMFIFAGAGALLYV
                     LLWIFVPSD"
     gene            complement(1171..2040)
                     /locus_tag="VGE92_06035"
     CDS             complement(1171..2040)
                     /locus_tag="VGE92_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEY1313412.1"
                     /translation="MREFDQKSITAAVIGRLAQSDDPRFKQVMISLITHLHDFVRDVK
                     LTEPEWMTAIQFLTDVGQTCTSKRQEFILLSDTLGVSVLVITLNHPADEGSAESTVLG
                     PFFWEGAPDLPRGCNLADGVKGEPAFYSGRVLSVDGRPLENALLDIWSGDGDGNYDMQ
                     IPGETGMKARGRIRTDADGRYWFRSIKPTFYPVPTDGPVGRMLRKMGRHPYRPGHIHM
                     IVSAAGHLPVTTHLFVAGSDYLDSDAVFGMKESLVAHFERHPAGVGPFGERMDTPFYC
                     VNYDFKLRPAGAP"
     gene            complement(2083..3261)
                     /locus_tag="VGE92_06040"
     CDS             complement(2083..3261)
                     /locus_tag="VGE92_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313413.1"
                     /translation="MTAPPGRPPVERRKSTAAAISTPFRFYDNRQKYLAFVTTCNEKS
                     AIARRAAHEVALLHPTPPAVRMFDAGMGDGTVIARIMRSTHRQFPTVPMFVVAKEISL
                     EDVRLGLEKLPDRLFEHPATVFVVTNLSYAEAPRLMPRDAQQAAALNWREVRLTGSTV
                     HEYAEQIDDLGASLASGWQTTPSAKTGNPLVVRPSVLVIYREDQKFILDAVIPKPGRI
                     FEHYDLVVASQPWRARMGAEFKVQKVLAPLTRSLAAGGRLLAIQSYGQDPGLEIIQQL
                     WPEERPFQVGRQELLSTLRRELGREARDFKLAVPDDDKAVFRYEMHTLPSEIGDRIGT
                     STLFAAWNAAIYVNQIEDERLDAVVTNGAYLAATQNVLKTHGGLWFNDEAFVVTRRRT
                     "
     gene            complement(3279..4424)
                     /locus_tag="VGE92_06045"
     CDS             complement(3279..4424)
                     /locus_tag="VGE92_06045"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEY1313414.1"
                     /translation="MRHSTDRILTTHVGSLPRSQAVTDVLFARERNEVSDAHAAASVI
                     AEAVTEVVRRQVQVGIDVVSDGEMSKISYATYIADRFAGFGGDTPREPGQDLVEFPGL
                     LKKLAERGSTAKYRRPRCVGEIRVKDLAPLQADLDNFHSAIAAAAPTEAFMNAASPGV
                     VALFQPNDYYRSQEDYLSALAEALRVEYETIVRAGVLLQIDAPDLAMGRHTMYRERSL
                     EEFEGLAARHIEVLNHALRNVPAERVRMHVCWGNYEGPHHHDVPMERLLPIVLKAKPQ
                     GLLFEAANPRHAHEWAVFKDAKIPQDKVMIPGVLATTTNYIEHPQLVAERIERFAHIV
                     GRERVMAGTDCGFGTFAGFGPVEPEIAYLKLKSLVDGAQIASGRLWR"
     gene            complement(4442..4978)
                     /locus_tag="VGE92_06050"
     CDS             complement(4442..4978)
                     /locus_tag="VGE92_06050"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY1313415.1"
                     /translation="MLKSNKSPMRLHHNAYVTRDQEKTRQFYEDVIGMPLVATWCESD
                     DLFGKVRTYCHTFFAMADGGALAFFQFANPDDQAEFGPKIPASPFHHIALAVDAQTQA
                     AIEERIAAAGIKPPETYVLEHGYCRSVYVHDPNGLILEFTLDSPQAVAINNARRADAH
                     AELKRWIAGDHTSNNTYR"
     gene            complement(4991..7174)
                     /locus_tag="VGE92_06055"
     CDS             complement(4991..7174)
                     /locus_tag="VGE92_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1313416.1"
                     /translation="MAQTSDLTEIIVTARKRDETFQSVPITMNVFAAQEIQAAGIENP
                     RDFVAMVPNMTLVEVQNVGNAFITVRGISQARNSEPSVAVLVDGVLETNPYEFDQELT
                     DVQQIEVLKGPQGALYGRNAIGGAIIIQTADLADHFEGTGKVGVGNGVSEKAQFAVSG
                     PIDSAGTLKYRASLNFYNTDGYLQNTYLDRKADPYRDYSGRLRLLWKPADDWSADLRV
                     FRDHVETTAYYYVIPRDDEANVFSSFSTPPNANDVTSPIQNNNLGSDTRDITDIALKL
                     DFNVGYGTFTSVSDYNRTKEIDTGDAYDFRPITTSIFYNYFAPLYYGSLPAADGGPFD
                     ESQSQFIDVKTYSEELRFTASPINGFSWIGGAYFVHTDRFISTGNLVDRGDGIPNVYQ
                     TPLVDPTNPIATNTNVTFLADSQSNNAWAVFGDATYEFTSQWELDAAIRYDQDQRHNT
                     TDTPTQFLIDPADSHTGEVRNATFSAVQPKGTLRFKPTDNLTFYGGWSRGFRSGGFNQ
                     TGVGAAAAAAGQLGVHDLFQAEVADTWEVGAKSQFLDRRLSANLALYHTDSHNGYFFF
                     YSATTSTQNLGNLDATYKGAELELTARATNWLDLYANFGYTDGRITAMQDPTVVGNKP
                     PLLTKDTVNAGFQVHQRVADGLNGTLRLDYQEIGRTWWDPYNVTSRDPINLINLRTGL
                     EGQRWTVTAWSKNLTNKIYNAEFSAGGFLWRAPPRRYGVDYTFKF"
     gene            complement(7309..>8176)
                     /locus_tag="VGE92_06060"
     CDS             complement(7309..>8176)
                     /locus_tag="VGE92_06060"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY1313417.1"
                     /translation="GTAARLGASHERLKGLNERLIYTSISGFGSSGPYVDRPSYDSVA
                     QALSGFLSVVVDTSRPRFLGPALADAITGIYAAYGVLGGLVQRGRTGSGCLVEVSMLE
                     AMAHFAVEPFAAFFALGITPTSSDRPRLAQAYILRTADARLIAIHLSSLEKFWDGLVM
                     ALNAPELASDVRFNPRTARIAHYEELNEELDRRFAQHDLAHWIEQLGRNDVPYAPIKA
                     IDEVIHDPQVEHLGLIVPVEAAHSGSQAVRPPVQFGGVRAQSVRAAPALDQHGAAIRE
                     ALARDAAWPAMA"
BASE COUNT         1534 a         2609 c         2479 g         1554 t
ORIGIN      
        1 gaccgagtca gtccacccgt aggcgctctt cagcccgacc acgtaaccga gcatcgcgag
       61 gaaggcgatt ccgcccgcga gcgttttcaa gatgacagcg gcagtggttg cggcaccggc
      121 gtgcgggtcc ggcttggaca tgagcacttg cgcggcgcat accagaagaa aacatgcaat
      181 cgtattgccc gacattcgcc ccggataaga ggcatcgaga cccacgaatg gcaccgcaaa
      241 cagcctatcg ataccgagat ccagaccaag tgcgtattca acaaattcgg cgcccgcaat
      301 acaggtcacg gcgaccgcca cccccatcac gatgcctcgg cggcgcagca ccaagcccaa
      361 ctctcccact cccaggacaa tgaagatcaa tgcggtattg aaatgggtgg gagcatcggg
      421 agatagttgg accaaaatgg gtatgcgcag ccaccatccg atgcacaccg acgcgccgtt
      481 gccgatgatg aacagcgcgg tgcctgcgac gatccaatct gcagttgaga aacgacgcaa
      541 aggctcaatc aaggcagatc ctctggcggg gcgcctgtcc gtacgcgcga gcaactgtct
      601 ctcaggcgat cgaccgagag ttatccaagc agcctacagt gagacagcca gcggggatgt
      661 agggaaactg ccatcggcct gcacaaggcg cgcgacgacc acatgttctg tggaccatat
      721 cacacgtatc gatcagtcgc ttggcacgaa gatccacaag agcacgtaca acagcgctcc
      781 ggcgcctgcg aaaatgaaca tgatcgcgaa caggagccgc cacacccacg cctccacccc
      841 cgttgcgcgc gccatgccgc cgcatacgcc gccgatgatt cgatcgctgc ggctgcgccg
      901 gaaggcattg accgccgcca ccaccggctc gttcggtggc gcggcttgac cggtctgccc
      961 ggcctgctca ttcaacagcc gcgccttcgc gcgcgcgaac tcttcttccg tcaatgtgcc
     1021 gctggcacgc agctctgcca gcctgctcag ttcgtcgctc aaggtcatct tagtctccgt
     1081 tgaaaggcgc gcgtcggcgg cgcacacaac ccgcgcttga tggcgctcat actcccataa
     1141 cacacgatgg gggtgaaaag ttgcaacggg ctagggtgcg ccggccggcc tcaatttaaa
     1201 gtcgtagttg acgcagtaga aaggagtgtc catgcgctcg ccgaaaggac ccacgccggc
     1261 ggggtgccgc tcgaaatgcg ccaccagaga ctccttcatc ccgaacaccg catccgaatc
     1321 caagtagtcg ctgccggcaa cgaacaagtg ggtggtgacc ggcaagtgtc cggccgcgga
     1381 tacgatcata tgaatgtgcc ctggacgata cggatggcgc cccatcttgc gcagcatccg
     1441 tcccaccggc ccgtcggtcg ggacaggata gaaggtcggc ttgatcgaac gaaaccagta
     1501 gcgcccgtcg gcgtcggttc tgatcctgcc gcgcgccttc attcccgtct ccccggggat
     1561 ctgcatgtcg tagttcccgt cgccgtcgcc ggaccagata tcgaggagcg cgttctcgag
     1621 cgggcggcca tcgacgctga gcacgcggcc cgaatagaat gcgggctcac ccttgacccc
     1681 gtcggccaga ttgcagcccc gcggcaggtc gggagccccc tcccagaaga acgggcctag
     1741 aaccgtcgat tccgcactgc cctcatccgc gggatgattg agggtgatca cgagcaccga
     1801 tacgccgagg gtgtcggaca ggaggatgaa ctcctgacgt ttgctcgtgc acgtttggcc
     1861 cacgtcggtg aggaattgaa tggccgtcat ccattcgggc tcggtgagtt tcacgtcccg
     1921 aacgaagtcg tgaagatgag tgatcagcga gatcatgacc tgcttgaatc tcggatcgtc
     1981 gctttgcgcg agccgcccga tcaccgctgc ggttatgctc ttctggtcga actcgcgcat
     2041 gctgtccacc ctatcctcat ggcgatctaa tgaccggcgg tatcaagtgc gccgccgcgt
     2101 gacgacgaat gcctcatcgt tgaaccacag ccccccatgc gtcttgagga cgttttgcgt
     2161 ggccgcgagg taggcgccgt tcgtcaccac cgcatccagg cgttcgtctt cgatctgatt
     2221 cacatagatc gccgcattcc aggcggcgaa cagcgtcgag gttccgatcc ggtcgccgat
     2281 ttcggagggc aaggtgtgca tttcgtagcg aaacacggcc ttgtcgtcat cggggacggc
     2341 gagcttgaag tcccgcgcct cgcgtccgag ttcgcggcgc agcgtgctga gcagttcttg
     2401 acggccgact tggaacggcc gctcctcggg ccacaactgt tggatgatct cgagccctgg
     2461 atcctgtcca tacgactgga tggccaacag cctgccgccg gcggccagac tgcgtgtgag
     2521 cggcgccagc actttctgca ctttgaactc ggctcccatg cgcgcccgcc acggctgcga
     2581 ggcaacgacc aggtcgtagt gctcgaagat ccgcccgggc ttcgggatca ctgcatccag
     2641 aatgaacttc tgatcttcgc gataaatcac cagcacggag gggcgcacca ccaacgggtt
     2701 acccgtcttg gcgctcggcg tggtctgcca gccgctggcg agggaggcgc cgagatcatc
     2761 gatctgctcg gcatattcgt ggacggttga gccggtcagc cgtacctcgc gccaattgag
     2821 cgccgccgcc tgctgcgcat cgcgcggcat cagccgcggc gcttcggcat agctcagatt
     2881 ggtcacgacg aacacggttg ccggatgctc gaagagtcga tcggggagtt tctcgagacc
     2941 caaacgcaca tcctcgagac tgatttcctt ggcgaccacg aacatcggga cggtcggaaa
     3001 ttgccggtgt gtgctgcgca tgatccgtgc aatcacggtt ccatcgccca tgcctgcgtc
     3061 gaacatgcgc acggccggcg gggtcggatg cagcaaggcc acttcgtgag ccgcacgccg
     3121 ggcgatcgcg gacttctcgt tgcaggtcgt gacgaaggcc aggtattttt gccggttgtc
     3181 gtagaagcga aacggcgtcg aaatcgctgc cgccgtactc ttgcgccgct cgaccggcgg
     3241 acgcccgggc ggggcggtca tgtcagttac acctgcgtct agcgccacag acggccgctg
     3301 gcaatctgcg ccccatcgac cagtgatttg agcttcaaat aggcgatttc cggttcgacc
     3361 ggaccgaaac ccgcaaaagt gccgaagccg cagtcagtcc ccgccatgac ccgctcgcgt
     3421 ccgacgatgt gcgcgaagcg ctcgatgcgc tccgcgacca gctgcggatg ctcgatgtag
     3481 ttcgtggttg tcgccagcac ccccggaatc ataaccttgt cttgcgggat tttcgcgtcc
     3541 ttgaacacgg cccattcgtg cgcatgccgc ggattcgccg cttcgaacaa cagtccctgc
     3601 ggcttcgcct tgagcacgat cggcagcagc cgctccatcg gcacgtcatg gtgatgcgga
     3661 ccctcgtagt tgccccaaca aacatgcatg cgcacgcgct cggccggcac atttctcaag
     3721 gcatgattca acacctcgat gtgccgcgcc gcgaggcctt cgaactcttc gagcgatcgc
     3781 tcgcggtaca tggtgtgccg acccatggcc agatccggtg cgtcgatctg cagcagcaca
     3841 cccgcccgca cgatggtttc gtactccacg cgcagcgcct cggcgagtgc cgacaagtag
     3901 tcctcctgcg aacggtagta gtcgttcggc tgaaacagcg ccaccacccc gggggaggct
     3961 gcattcatga aggcctcggt cggcgcagcc gcggcgatcg ccgaatggaa gttgtcgagg
     4021 tccgcttgca acggcgcgag atccttgacg cgaatctcgc cgacacagcg cggccggcga
     4081 tacttggccg tcgaaccccg ctccgccagc ttcttcagca accccggaaa ctcgaccagg
     4141 tcttgtccgg gctcgcgcgg cgtatcgccg ccgaatcccg cgaagcgatc ggcgatgtag
     4201 gtggcatagc tgatcttgct catttcccca tcgctgacca catcgatgcc gacttgaacc
     4261 tgacgccgca cgacttcggt cacggcctcg gcgatgacgg acgccgctgc gtgcgcgtcg
     4321 ctcacctcgt tgcgttcgcg cgcgaacagc acatcggtca cagcctgact gcgcggcagg
     4381 ctgccgacat gagtcgtcaa gatgcggtcg gtgctgtgcc tcatgctgcg ctggccggcc
     4441 atcaccgata tgtgttgttg gacgtgtgat cgcccgcgat ccaccgtttc agttcggcgt
     4501 gcgcgtcggc gcgccgcgca ttgttgatcg caaccgcctg cggactgtcg agcgtgaatt
     4561 ccaggatcag cccgttggga tcgtgcacgt aaaccgagcg gcagtagcca tgctccagga
     4621 cgtaggtttc cggcggcttg atgccggcag cggcaatccg ctcctcgatg gccgcttgag
     4681 tctgcgcgtc caccgcgagc gcgatatgat gaaacggcga ggccggtatc ttcggcccga
     4741 attccgcctg gtcatcgggg ttcgcgaatt gaaagaacgc gagcgcgccc ccgtcggcca
     4801 tggcaaagaa ggtgtggcag tacgtgcgca ccttgccgaa cagatcgtcc gactcgcacc
     4861 aggtggcgac caggggcatg ccgatgacgt cctcgtagaa ttggcgcgtc ttctcctggt
     4921 cgcgcgtgac ataggcgttg tgatgcagcc gcatcggaga cttatttgac ttgagcatgg
     4981 cacttcccca ttagaacttg aaggtgtaat ccaccccgta gcggcgcggc ggggctctcc
     5041 acaggaagcc tcccgcggag aattcggcat tatagatttt gttggtcaga ttcttcgacc
     5101 aggcggtaac cgtccaccgt tgaccctcga gtccggtgcg caggttgatc aaattgatcg
     5161 gatcgcgcga ggtcacgttg tacggatccc accaggtacg gccgatttct tgatagtcca
     5221 gacgcagcgt gccgttgagc ccgtcggcga cgcgctggtg gacctggaag ccggcattca
     5281 ccgtgtcttt ggtcagcagc ggcggtttgt tgcccacgac cgtgggatcc tgcatggcgg
     5341 taatcctgcc gtccgtgtag ccaaagttgg cgtataggtc gagccagttg gttgctctcg
     5401 ccgtcaactc gagctcggcg cccttgtaag tggcgtcgag attgccgaga ttctgcgtcg
     5461 aggtcgtggc gctgtagaag aagaaatagc cgttgtgcga gtcggtgtga tagagcgcca
     5521 gattcgcgct caaccgccgg tcgagaaact ggctcttggc acccacttcc cacgtgtcgg
     5581 ccacctcggc ctggaacaaa tcatgaacgc caagttggcc tgccgctgcc gcggctgcgc
     5641 cgacgccggt ctggttgaag ccgccgctgc ggaacccgcg gctccagccg ccgtagaagg
     5701 tcaagttgtc ggtcggcttg aagcgcaacg tgcccttggg ctgcaccgcg ctgaaggtgg
     5761 cgttgcgtac ctctccagta tgggaatccg ccggatcaat caggaattga gtcggggtat
     5821 cggtggtgtt gtgccgctga tcctggtcgt agcggatggc cgcatcgagc tcccactgcg
     5881 acgtgaattc ataggtcgca tcgccgaaca ccgcccaggc gttgttgctc tgcgagtccg
     5941 caaggaacgt gacgttcgtg ttggtggcga tcggattggt cggatcgacc agcggggtct
     6001 gatagacgtt cgggataccg tctccgcgat ccaccagatt gccggtggag atgaagcgat
     6061 ccgtgtgcac gaaatacgct cccccgatcc acgagaaacc gttgatcgga ctggccgtga
     6121 accgcagctc ctcgctatag gtcttcacat cgatgaactg gctctggctc tcatcgaacg
     6181 gcccgccgtc ggcggccggc aggctgccgt agtagagcgg cgcgaagtag ttataaaaga
     6241 tcgacgtcgt gatcggccga aagtcatagg catctccggt atcgatctcc ttggtgcgat
     6301 tgtaatccga cacggatgtg aaggtgccgt agccgacgtt gaaatcgagc ttgagcgcga
     6361 tatccgtgat gtcgcgcgtg tccgagccga gattgttgtt ctggatcgga ctcgtcacat
     6421 cattcgcatt cggcggcgtc gagaagctcg agaacacgtt ggcctcgtcg tcacgcggga
     6481 tgacgtagta atacgcggtc gtttccacgt gatcgcggaa cacgcgcaga tccgcgctcc
     6541 aatcatcggc gggtttccac aatagccgca gccgcccgga gtagtcgcgg tacgggtcgg
     6601 ccttgcggtc gagataggta ttctgcagat agccatccgt attatagaaa ttgagcgagg
     6661 cgcgatattt gagcgtcccg gccgaatcga tcggcccgct caccgcaaac tgggccttct
     6721 ccgacactcc gttacccacg ccgaccttgc cggtaccttc gaaatgatcc gcaaggtccg
     6781 cggtttggat gatgatggcg ccgccgatgg cgttgcgccc gtacagtgcg ccctgcggcc
     6841 ccttcagcac ttcgatctgc tggacgtcgg tgagctcctg atcaaactcg tagggattgg
     6901 tttcgagcac gccgtcgacc agcacggcaa ccgaaggctc gctgttgcgc gcctgcgaga
     6961 tgccgcgcac ggtgatgaag gcgttgccga cgttttgcac ctcgaccagg gtcatattgg
     7021 gcaccatggc gacgaaatcg cgcggattct cgatgcccgc agcctggatc tcctgagccg
     7081 cgaacacatt catcgtgatc ggcacgctct gaaatgtttc gtcgcgcttg cgcgcggtga
     7141 cgatgatctc ggtcagatcg ctcgtttggg ccactaccgg gagcgccgcg gtcaggcaaa
     7201 cccccgttgt cgccagcacc gtaacccatc tccccgttgc aatgcgcata gtgttctccg
     7261 ttttcgattt gtatctctcg gccgcaccca gccccccgta acgccgcctc aagccatagc
     7321 cggccacgcc gcgtctcgcg ccaacgcttc acgaatcgcc gccccgtgct gatcgagtgc
     7381 cggagccgcc cgcaccgact gcgcacgcac accgccgaac tgcaccggcg gtcgtaccgc
     7441 ctggctgccg ctgtgtgcgg cttcgaccgg cacgatgagt cccagatgct cgacctgagg
     7501 atcgtgaatc acctcgtcga tcgcctttat cggcgcatac ggaacatcat tgcgcccgag
     7561 ctgctcgatc caatgcgcca ggtcgtgctg cgcgaatcgc cggtcgagtt cctcgttcaa
     7621 ttcttcgtag tgggcgattc gcgcggtacg aggattgaag cgcacgtcgc tcgcaagctc
     7681 gggcgcgttc aacgccatga ccagtccatc ccagaatttc tccagcgacg agagatgaat
     7741 ggcgatcagg cgcgcatcgg cggttctcaa gatatacgcc tgcgcgagcc gcggacgatc
     7801 gctcgacgtc ggcgtgatgc cgagcgcgaa gaaagccgca aacggttcca cggcaaagtg
     7861 cgccatggcc tcgagcatcg acacctcgac cagacagccg ctgccggttc ggccgcgctg
     7921 taccaagcca cccagcacgc cgtacgccgc gtagataccg gtaatcgcat cggcgagagc
     7981 gggacccaag aatcgcggcc ggctggtatc gaccaccacg ctcaaaaatc cgctcaacgc
     8041 ctgcgccacc gagtcgtagc tcggccgatc cacgtacgga ccgctcgagc caaaaccgct
     8101 gatcgatgtg taaatgagcc gctcgttgag ccccttcaag cgctcgtggc ttgcgccgag
     8161 acgcgcggcg gtgccc
//
LOCUS       DASWZT010000219         2442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_1638, whole genome shotgun sequence.
ACCESSION   DASWZT010000219 DASWZT010000000
VERSION     DASWZT010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VGE92_06065"
     CDS             <1..862
                     /locus_tag="VGE92_06065"
                     /inference="COORDINATES: protein motif:HMM:NF044545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313418.1"
                     /translation="ARLHMWQTGYPFHVMHNYRVSAREALDFSRLVHRVDTETLSKIE
                     VVNAARRPLLAYAALVMENLVRTIKPKQVIISVLGVREGLLYSLLKPNERARDPLISA
                     AADLNVLRSRSPHHGEELIAWTDRFVASSGLDENADERRLRHAACLLGDIGWRAHPDY
                     RGEQSMNIIAHGAFVSVDHPSRAYLALSVYFRHAGLSEEDLSPRLRELATTRMLDRAR
                     VLGAAMRVAYILTAGQSGVLPKTPMQVKRGKLILKLPGRYSRLSSERLFSRVRQLARL
                     VGRDPEIAVS"
     gene            complement(962..2110)
                     /gene="rnd"
                     /locus_tag="VGE92_06070"
     CDS             complement(962..2110)
                     /gene="rnd"
                     /locus_tag="VGE92_06070"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEY1313419.1"
                     /translation="MITTTKELAEVCARMARHPYVTVDTEFLRETTYYPLLCVAQLAS
                     SDEAVVVDALAAGIDLGPLFDLMTDEKVVKVFHAARQDIEIVWNMAKKIPHPIFDSQV
                     AAMVLGYGDSISYDQLVQRITGNMLDKSHRFTDWTRRPLSDAQVTYALSDVTHLRDVY
                     LKLAADLAKRERTSWVEAEMGVLTSPETYRMDPERAWERLKSRVRKPKELAVLMEIAA
                     WREREAQTRDVPRSRVLKDDLVGDIAIQAPTTIERLGSLRSLPKGFERSRWGEAIVDA
                     VKRGLERDPKTLPRLDRFRPAPNGAATVELLKVLLRMTAEGHGVAAKVIATVDELDRI
                     AADDDADVPALKGWRRELFGEKALALKHGKLALAVDRGRVVTVEKTSR"
     gene            complement(2149..>2442)
                     /locus_tag="VGE92_06075"
     CDS             complement(2149..>2442)
                     /locus_tag="VGE92_06075"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY1313420.1"
                     /translation="PLVAVVFADGAYGNVRRIQQEQFGNRLIASDLTNPDFVKYAESF
                     GAAGRRVRSPQELGVALQAAFARCEPTLIEVPVGPLPSPWEFIHMPRIRGKLP"
BASE COUNT          392 a          789 c          820 g          441 t
ORIGIN      
        1 ggcgcggctg cacatgtggc agaccggcta tccgttccac gtcatgcaca actaccgcgt
       61 ctcggcgcgc gaggcgctcg acttttcgcg gctggtgcat cgcgtcgata cagagacgct
      121 gtccaagatc gaagtcgtca atgctgcgcg ccggccgctg ctggcctatg ccgcgctggt
      181 gatggaaaat ctggtgcgca ccatcaagcc gaagcaggtg ataatctcgg tgctcggcgt
      241 gcgcgagggg ctgctctatt cgttgctcaa gccgaacgaa cgggcgcgcg atccgctgat
      301 ctcggcggcg gccgatctca acgtgctgcg ctcgcgctcg ccgcatcatg gcgaggaact
      361 gatcgcctgg accgaccgct tcgtggcgtc gtcgggcctg gacgaaaacg ccgacgagcg
      421 acggctgcgt catgccgcgt gtctgttggg cgatatcggc tggcgtgcgc acccggatta
      481 tcgcggcgag cagtcgatga acatcatcgc tcacggcgcc ttcgtgtcag tcgatcatcc
      541 aagccgggcc tatctggcgc tgtcggtcta tttccggcac gccggcttaa gcgaggagga
      601 cttgtcaccg cgcctgcgcg agctcgccac cacccgcatg ctggatcggg cgcgtgtgct
      661 gggcgccgcc atgcgcgtcg cttatattct taccgccggg cagagcggcg tgctgccgaa
      721 gacgccgatg caggtcaagc gcggcaaact cattttgaag ctgcccggcc gctattccag
      781 gctctcgagc gagcggctgt tcagccgggt gcgccagctc gcgcgtctgg tcggccgcga
      841 tccggaaatc gcggtgtcct gaccgcggta gccggttcac gacatcgacg gctcgggccg
      901 cgcccgtgga cgtcctgcag ccgaaggtga tggataacgc ggtaagcgag ccgatcgtac
      961 gttagcggct agttttttcg accgtcacga cacggccccg gtcgaccgcg agcgccagct
     1021 tgccatgctt gagcgcgagc gccttctccc cgaacaattc gcggcgccag cctttgagcg
     1081 cgggcacgtc ggcgtcatcg tcggcggcga tgcggtcgag ctcgtccacc gttgcgatca
     1141 ccttggcagc gacgccgtgg ccctcggcgg tcatacgcag cagaaccttc aacagctcga
     1201 cggtggccgc gccattgggc gccggacgga agcgatcgag ccgcggcagc gttttggggt
     1261 cgcgctcgag cccgcgcttg accgcgtcga cgatggcctc gccccagcgc gagcgctcga
     1321 agcctttggg cagcgagcgc aggctgccca gccgctcaat tgtggtcggc gcctggatgg
     1381 cgatgtcgcc aaccaaatcg tccttgagca cgcgcgagcg cggcacgtcg cgtgtttgcg
     1441 cctcgcgctc gcgccaggcg gcgatttcca tgagcacagc cagttccttc ggcttgcgca
     1501 cgcggctctt cagccgctcc caggcgcgct ccggatccat gcggtaggtt tcgggcgagg
     1561 tcagcacgcc catctcggct tcgacccagc tggtgcgttc gcgcttggcc agatcggcgg
     1621 cgagcttaag gtacacatcg cgcaaatggg tgacgtcgga cagcgcgtag gtgacctgcg
     1681 cgtcggacag cggccgccgc gtccaatcgg tgaagcggtg cgatttgtcg agcatgttgc
     1741 cggtaatgcg ttgcaccagc tggtcgtagg agatcgagtc gccatagccg agcaccatgg
     1801 cggctacctg gctatcgaag atcggatgcg gaatcttttt cgccatgttc cagacgattt
     1861 cgatgtcctg acgcgcggca tgaaaaacct tcaccacctt ctcgtccgtc atcaggtcga
     1921 acaggggccc gaggtcgatg cccgcggcca gcgcgtcgac cacgacggct tcgtcggacg
     1981 aggccagctg cgccacgcag agcaggggat aataagtggt ctcgcgcagg aactcggtat
     2041 cgacggtgac gtaaggatgc cgcgccatgc gcgcgcaaac ctcggccagt tccttggtgg
     2101 tggtgatcaa atccatgttg gcgggcaacc taacccgatc cggggctgtc atggcaactt
     2161 ccctcggatc cggggcatgt ggatgaattc ccagggcgag ggcagcggcc cgaccggcac
     2221 ctcgatcagc gtcggctcgc acctggcgaa ggcggcctgg agcgcgacgc ccagttcctg
     2281 cgggctgcgg acgcggcgcc cggctgcgcc gaagctttcg gcgtatttga cgaaatcggg
     2341 gttggtgagg tccgaggcga tcaggcggtt gccgaattgc tcttgctgga tgcgccgcac
     2401 attgccgtag gcgccgtcgg cgaacaccac cgccaccagc gg
//
LOCUS       DASWZT010000220         3651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_88697, whole genome shotgun sequence.
ACCESSION   DASWZT010000220 DASWZT010000000
VERSION     DASWZT010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88697"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1501
                     /gene="cysC"
                     /locus_tag="VGE92_06080"
     CDS             <1..1501
                     /gene="cysC"
                     /locus_tag="VGE92_06080"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF004035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEY1313421.1"
                     /translation="RKGVLTQTRRHSYLAALLGIRHIVVAVNKMDLVDYSQEIFDKIE
                     RDYREFALQIKLESITCIPVSAVLGDNVTSRSERTPWYAGPGLLQHLETIEVRDAAQD
                     TPFRMPVQWVNRPHPDFRGFAGLIAGGTVRRGDSVRVLPSGRSSTVARVLTGDGDVDF
                     AVAGQSVTLTLSGEIDVSRGDVLAAAAEPPAVANQFEATIIWMHDEPLLQGRAYLFKL
                     GTKTATATVSPIKYRVNVNTLEHLAAKKLELNDIGICGLELDCAIAFEPYRESRVLGG
                     FILIDRLTNNTVGAGLLHFALRRSQNVHWQALDVNKSARASLSHQKPCILWFTGLSGA
                     GKSTIANLVEKQLHAEGRQTYLLDGDNVRHGLNKDLGFTNQDRVENIRRVAEVAHLMV
                     DAGLIVLVSFISPFRSERQMARSLVEAGEFIEVFVDTPLSLAEARDAKGLYKKARRGE
                     LKNFTGVDSPYEAPENPEIRLDTTLASPELAATRVITFLRAAGVIDAAT"
     gene            complement(1543..1863)
                     /locus_tag="VGE92_06085"
     CDS             complement(1543..1863)
                     /locus_tag="VGE92_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313422.1"
                     /translation="MNPYKPGPVFGRPSTPSGEDPMMDFRARLVHQQTEAAERRRTDL
                     AEQCSRLKTAEERIRIWERIHEVTLPRDPAHRLVEIIAANTGLTDADVRDEQVRRAAP
                     RAAV"
     gene            complement(1950..2471)
                     /locus_tag="VGE92_06090"
     CDS             complement(1950..2471)
                     /locus_tag="VGE92_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HEY1313423.1"
                     /translation="MSLERPLANTYWVIPNRVLAGEYPGDPDEIAARERLIRLHRAGI
                     DSFIDLTEEGELPPYHHLLAKPSEYTRSAIVDSGVPNNVSQTQQILSTIRAAMGRGRR
                     VYVHCRAGIGRTGLIVGCFLAEEESNGKSALKILNTLWRQNERSASWPKVPQTAEQAD
                     YIRHWPKFGKRIA"
     gene            2627..3259
                     /locus_tag="VGE92_06095"
     CDS             2627..3259
                     /locus_tag="VGE92_06095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system exopolysaccharide export
                     protein"
                     /protein_id="HEY1313424.1"
                     /translation="MISSKPLKLREFLRLLTLGAAILAAGMPQRGVQAAEAVTPAPVS
                     ADYLIGPGDTLHVFVFQNDELSVTVPVRPDGKISTPLVEDMVAVGKTPSQLARDIERS
                     LTEYVKSPKVNIVVTIALSVYSQVRVIGQVKSPQSLPFRDGMTVLDAMLAVGGLGQFA
                     AGNRAHLVRTENGKQTEIKVKLDALVNNGDMKQNLSLKPGDVLVVPESRF"
     gene            3276..>3651
                     /locus_tag="VGE92_06100"
     CDS             3276..>3651
                     /locus_tag="VGE92_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313425.1"
                     /translation="MKQELERVFDEVQSAWRFRWLGLAAACIVAVVGWCLVFALPDRY
                     EADARVFVDTRTALKPALQGLTTDQNVDAQINYVRQSLLEGPQLETIAKETGVLDASV
                     TDERIRAKILDRLSDRIALTVSS"
BASE COUNT          706 a         1067 c         1168 g          710 t
ORIGIN      
        1 tcgcaagggc gtgctcacgc agacgcggcg gcatagttat ctcgcggcgc tgctcgggat
       61 tcgacacatc gtcgtcgccg tcaataaaat ggatctggtg gactactcgc aggaaatatt
      121 cgacaaaatc gaacgcgact atcgcgaatt cgccctgcag atcaagctcg aatcgatcac
      181 ctgcatcccg gtctcggcgg tgctcggcga caatgtgacc tcgcgcagcg agcgcactcc
      241 gtggtacgcg ggcccgggac ttttgcagca cctcgagacg attgaagtgc gcgacgccgc
      301 acaggacacg ccgtttcgca tgccggtgca gtgggtaaat cgtccacacc cggattttcg
      361 cggcttcgcc ggcctgatcg ccggcggaac ggtgcgccgc ggcgactctg tgcgggtgct
      421 gccgtcggga cgcagcagca ccgtggcgcg cgtgttgacg ggcgacggcg acgtggattt
      481 cgcggtggcc ggacagtccg taaccttgac cctgagcggt gaaatcgacg tgagccgcgg
      541 cgatgtgctc gcggccgcgg cggagccgcc ggcggtcgca aaccagttcg aagcgaccat
      601 catttggatg cacgatgagc cgttgcttca gggacgcgcc tacctgttca agctcggtac
      661 gaaaacggcg accgcgacgg tgtcgccgat caagtatcga gtcaacgtca acaccctgga
      721 gcatttggcg gcgaagaaac tcgaactcaa cgatatcggc atatgcggtc tggagttgga
      781 ctgcgccatc gcgttcgaac cctatcgaga aagccgcgtc ttgggcggtt tcatcttgat
      841 cgatcggctc accaacaata ccgtcggtgc cggactgctg cactttgcgc tgcgccgctc
      901 acagaacgtg cattggcagg cgctcgatgt gaacaagagc gcgcgcgcga gtctcagcca
      961 tcaaaagccg tgcatcctct ggttcaccgg gctgtcgggc gccggcaaat cgacgatcgc
     1021 caatctggtg gagaagcagc tgcatgccga gggccggcaa acgtacttgc tggacggcga
     1081 caacgttcgc catggactca acaaggactt gggtttcacc aatcaagacc gcgtcgagaa
     1141 cattcgccgc gtggcagagg tcgcgcattt gatggtcgat gccgggctga tcgtcctggt
     1201 atcgttcatt tcgccgtttc gctcggagcg gcaaatggcg cgcagcttgg tcgaggcagg
     1261 cgagttcatc gaggtgttcg tcgacacgcc gttgagtttg gcggaggcgc gcgacgccaa
     1321 ggggctctac aaaaaggcgc ggcgcggtga attgaagaac ttcaccggcg tcgattcacc
     1381 gtacgaggcg cccgaaaatc cggagattcg cttggacacg acgctggcgt cgcccgagct
     1441 ggcggcaaca agggtaatta ctttcttgcg cgcggccggc gtgatcgacg ctgcaacata
     1501 gaccgggtcg cgttagaccg ggtcgcgcta gaccgggtcg cgctagaccg cggctcgtgg
     1561 cgccgcgcgt cgcacttgct catcgcggac gtcggcgtcg gtgagccccg tattggcagc
     1621 gatgatctcg accagacggt gcgcagggtc acgcggcagc gtgacttcgt ggatccgttc
     1681 ccaaatgcga attcgttcct ccgcagtctt caaccgcgag cactgttccg ctaggtcggt
     1741 gcggcgccgc tctgcggctt cggtttgctg atgaacgagg cgtgcccgga aatccatcat
     1801 gggatcttcg ccggacggtg tggatgggcg cccaaaaacg ggacctggct tgtatggatt
     1861 cacgcgtatc tcctgaccga tcaacccctc aaagtaccac gctatggggt cgagcgaatc
     1921 ggtttcgtat cgcggcgacg ggaaggagct taggcgattc gcttaccgaa cttcggccag
     1981 tggcgaatgt agtccgcctg ttcggcggtc tgcggcacct ttggccaact ggcggagcgc
     2041 tcgttctgcc gccacagagt attgagaatt ttgagagccg atttgccgtt ggattcttct
     2101 tcggcgagaa agcagccaac gatcagcccg gtacgcccga tgcccgcgcg gcaatgcacg
     2161 taaacgcgcc gtccgcggcc catggccgcg cgaattgtcg acaggatctg ctgcgtctgc
     2221 gagacgttgt tgggtacgcc tgaatcgacg atggcggagc gcgtgtactc gctgggtttc
     2281 gccaacaggt ggtggtaggg cggcagctca ccctcctccg taagatcgat gaagctatcg
     2341 atacccgccc ggtggagacg aattaagcgc tcgcgggccg cgatttcgtc gggatccccg
     2401 gggtattcgc cggccaggac tcggttcggg atcacccaat aggtgttggc taaaggtcgc
     2461 tcgaggctca ccgcgggatg ttaccagcga ttcattttac gaaaaaagcc aagacagatg
     2521 acggacgtcg caaaattgca aagccgatcg tggcgcaggc ccgcacatcg tgtttagatt
     2581 cgtgaatata cggcgccccg cccgcagccg aggtaatata cgctccgtga ttagctccaa
     2641 acctttgaaa ttacgagagt tccttcgatt attgaccctg ggtgccgcga tcctggcggc
     2701 tggcatgccg caacgcggag tgcaggcggc ggaggcggtc actccggccc ccgtatcagc
     2761 ggactatttg atcggtccgg gcgacacctt gcatgtattc gtcttccaga acgatgagct
     2821 gtcggtcacc gtccccgtga ggcccgacgg caagatctcc accccgctgg tcgaagacat
     2881 ggtcgcggtg ggcaagacgc cctcgcaatt ggccagggac atagaaaggt ccctcaccga
     2941 gtacgtgaag tcgcccaagg tgaatatcgt tgtgaccatc gcgctcagcg tatacagcca
     3001 agtgcgggtc atcggccagg tgaaatcgcc gcaatccctg ccgtttcgcg acggcatgac
     3061 ggtactcgat gcgatgctgg cggtgggcgg cctgggacaa tttgcggccg gcaatcgcgc
     3121 gcatctggtg cgcaccgaga acggcaagca aaccgaaatc aaggtcaagc tcgatgccct
     3181 cgtcaataac ggcgatatga agcagaactt gtcgctcaag ccgggcgacg tgctggttgt
     3241 gcccgaatca cgattctgat ggtgaagtac cttttatgaa gcaggaattg gaacgagtct
     3301 tcgacgaggt gcaaagcgcc tggcgctttc ggtggctggg cttggctgct gcctgcatcg
     3361 tggcggttgt cgggtggtgc ctggtgttcg cgctgccgga tcgatatgag gccgacgctc
     3421 gcgtgttcgt cgacacacgt acagccttga agcccgcgct gcaggggctg accaccgacc
     3481 aaaacgtcga cgctcagatc aactatgtgc gccagtcgtt gctcgagggt ccccagctcg
     3541 agacgatcgc caaagagacc ggcgtcttgg atgcgtcggt taccgacgaa cgcatccgcg
     3601 caaagatctt ggatcggttg agcgaccgga ttgcgctgac cgtgtcgagc g
//
LOCUS       DASWZT010000221        10698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_69375, whole genome shotgun sequence.
ACCESSION   DASWZT010000221 DASWZT010000000
VERSION     DASWZT010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10698
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69375"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="VGE92_06105"
     CDS             complement(<1..954)
                     /locus_tag="VGE92_06105"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011532463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEY1313426.1"
                     /translation="MSVIREADVIASVADALQYISYYHTPDFIRAMGHAYQIEQSPAA
                     RDAIAQILTNSRMCAEGHRPICQDTGIVVVFVKVGMGVRWDASLDLEAMVNEGVRRAY
                     LNPDNKLRASIVSDPAFKRGNTRDNTPAVIHTEFVAGDAVEITVAAKGGGSENKSMFA
                     MLNPSDSVADWVLERVPEMGAGWCPPGMLGVGIGGSAEKAMLLAKKSLMEPIDIGELL
                     ARGPSSKLEELRLDIFQRVNALGIGAQGLGGLTTVLDVKVLDYPTHAASLPVAVIPNC
                     AATRHIHFTLDGSGPALLEPPNLDLWPKVTWSADASARRVSL"
     gene            1057..2553
                     /locus_tag="VGE92_06110"
     CDS             1057..2553
                     /locus_tag="VGE92_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY1313427.1"
                     /translation="MRVWRALPSSARGCALWSFLGIVGLWPAGWAAAAAPLALHECRI
                     EHPLRLTSIAARCANLRVPENRSEPGGATIELSIAVVPALNRRAAAAPLFLVAGGPGQ
                     GTKAMYAGLAGAFARVNRDHDIVLVDQRGTGDSTPLSCDYPDDWQAQDEMLQALRQAT
                     TACLQKFGARVRFFTSGAAVADLEAVRTALGYSALDLYGVSYGTRVAQLYMRRYPRAV
                     HAAILDGVLAPEQVIGPATPLDGERALDLIVARCKDAPECARAYPQLPHDLDDLRSRY
                     GMQKSTITLDDPDGGLPFQLQFNRSVLNASLRLLSYSASQASLLPTLVHRASQGSLGP
                     LAAQTVLTARQAAAQIAIGMQNSVVCSEDAPFFALSDAARAAIARTYQGTDQLDAFLE
                     VCKLWPRGAVDADLHSPLRSDIPTLLLSGEADPVTPPADAERAAQGLAHHRHLILSGE
                     GHGQVGTGCVPRLMAEFLEAAAPEKLDASCLKQHRPAAFFVSMTGPAP"
     gene            2550..3281
                     /locus_tag="VGE92_06115"
     CDS             2550..3281
                     /locus_tag="VGE92_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1313428.1"
                     /translation="MIEASHLSKRFGAIEAVRDVSFSARDGEITGLLGANGAGKSTCL
                     RMLYGVLTPDSGRASINGIDIRGETSKARAQLGVLPHAAGLYGNLTARENIYFFGSLQ
                     GIGRAALKSRTTELARALGMEEFIDRRAKGFSQGQRIKTALARALVHDPGHIVLDEPT
                     NGLDVMAIRNLREMLKALKAQGRCVLFSSHVMQEVAALCDRVVIIGHGRVLADASVQS
                     IREDSGAASLEEAFLKVLGDSHGAS"
     gene            3282..4460
                     /locus_tag="VGE92_06120"
     CDS             3282..4460
                     /locus_tag="VGE92_06120"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1313429.1"
                     /translation="MRALLTVFRKEFLENLRDRRTVVSALLFGPLFGPLIFGLMVSRM
                     LNQSLVESNEPLRLTISGGEHAPGLMRYLEAQGVELATAKLSESEAKAVVRGGSAKVV
                     LIIPAEYAARFAAAAPAPVLLVADSADSQTRKTAERARLFLGAYTSTISQLRLQIRGV
                     NPLLALPVAVNDLDVATPSGRAVVVLGFMTYFVLFAVLMGGLYLAIDSTAGERERGSL
                     EALLSLPVARSSLVGGKILATCAYMCMSLALSLTAFVCVFRFVPLESLGMSANLGAGT
                     ALIFFAICLPFVPLGAALMTFVASFTKSYREAQTYLASVLLVPTLPIAFASIYSLKTR
                     GSLMFIPSLSQHLLMTSVLKDEPVAALDVWVSAGASLALALLLIVLTARHWRRETMLG
                     "
     gene            4551..4766
                     /locus_tag="VGE92_06125"
     CDS             4551..4766
                     /locus_tag="VGE92_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313430.1"
                     /translation="MTDAAHARSNIAALLTILVLSAATMVWLFWHFPVITAVATLAIL
                     TSLGVSARLARLTDSEAKDLSRNQQGV"
     gene            complement(4775..6127)
                     /gene="mnmE"
                     /locus_tag="VGE92_06130"
     CDS             complement(4775..6127)
                     /gene="mnmE"
                     /locus_tag="VGE92_06130"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019580343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEY1313431.1"
                     /translation="MTPPDTIAAIATAPGRGAVGVIRVSGPQVPQIAVGILGELPNPR
                     RARLARFLDAHNASIDEGLALYFPAPASYTGEPVLEIQGHGGVVVLDLVLQRLLELGC
                     RLARPGEFSERAFLNGKIDIAQAEAIADLIEAGSAAAARAAVRSMQGEFSARVAALNV
                     GITSLRAYVEAAIDFPDEEIDFLSDAALRERLAAVFAGFASVGAAAAQGALLREGLNV
                     VIAGKPNAGKSSLMNRLAGDEVAIVTEVPGTTRDVLRCQVHLDGLPVNLIDTAGLRSV
                     DDVVEAEGVKRARSEIARADRVLYIVDAGITDQLGLAGSELASLPAGIPVTLVINKID
                     LAGLPARVDEASVPPRVFLSARFGTGMDLLRAHLQTSAGYTPSESGALAARRRHLNAL
                     QRARQHVQSAANTLSSTRAFELFAEDLRLAQHALGEITGEFTSEDLLGEIFGSFCIGK
                     "
     gene            complement(6134..7837)
                     /gene="yidC"
                     /locus_tag="VGE92_06135"
     CDS             complement(6134..7837)
                     /gene="yidC"
                     /locus_tag="VGE92_06135"
                     /inference="COORDINATES: protein motif:HMM:NF002352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEY1313432.1"
                     /translation="MLLWIALGTILFYCYQVWMHDYPPSLNSVATSTTGAPVSTLGDS
                     VPQAASAVTPAAPAVAVPPAAEPPPLGGAAPGVVPAAAPDAGPMQPVHVTTDVLDVVI
                     NLKGGELDQADLKQYPLRKDTPNIPVRLLSREPPNTLYLLQTGLTGDAGQAAPTHLAT
                     FTSAAQSFELAAGAQELRVPLTFTDGQGLTVTKTFVFTRGLYSIDLIYDVQNGGTAPR
                     KLASYSQFLRHWEHASRSYFDVETYSFKGPAVWDGRKSHDLNVESDSDSKFSETITNG
                     WLASLQHQFVSAIVPPPDQPYKYQLQVRDKEYLLSATGPVSEVAAGAQAQFREKLFVG
                     PKLQSQLAATAPNLERTVDFGILTVLAQPLFLGLNWVHRLIGNWGWSIIFVTAIIKLI
                     FYPLSQASGRSMAKMRTLAPRMKQIQDTFKDDREKLGRAMMELYKKEKINPLAGCLPM
                     LVQIPFFISFYRVLLESVEMRQAPFLMWVNDLSSRDPYFVLPLLMGGAMFAQFKLNPA
                     PPDPMQAKIMQFMPLVMTGMMAWFPAGLVLYWLTNTLLTILQQWRVNQVVEAEAAKPR
                     S"
     gene            complement(7845..8081)
                     /gene="yidD"
                     /locus_tag="VGE92_06140"
     CDS             complement(7845..8081)
                     /gene="yidD"
                     /locus_tag="VGE92_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEY1313433.1"
                     /translation="MRMSQLLMWVIRGYQLALSPLLGPRCRFYPSCSCYAHAAIERHG
                     AVRGVWLGLRRVLRCHPFNAGGYDPVPDKRTINA"
     gene            complement(8072..8437)
                     /gene="rnpA"
                     /locus_tag="VGE92_06145"
     CDS             complement(8072..8437)
                     /gene="rnpA"
                     /locus_tag="VGE92_06145"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEY1313434.1"
                     /translation="MPNFIKRLTFEPRKRLHQPAEFRDVKRRGRKFADAFFSLSVLAN
                     HESYPRLGLSIATRTFGTAVARNRIKRITRESFRLNQHSLPPVDVTVSARDAARQAKA
                     CDLRASLDQHWKSISQRCV"
     gene            complement(8462..8596)
                     /gene="rpmH"
                     /locus_tag="VGE92_06150"
     CDS             complement(8462..8596)
                     /gene="rpmH"
                     /locus_tag="VGE92_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEY1313435.1"
                     /translation="MKRTFNPSNLHRARTHGFRARMATKSGRRLLARRRAKGRKRLCP
                     "
     gene            8771..10129
                     /gene="dnaA"
                     /locus_tag="VGE92_06155"
     CDS             8771..10129
                     /gene="dnaA"
                     /locus_tag="VGE92_06155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEY1313436.1"
                     /translation="MSESLWNRCLRVLENELPEQQFNTWVRPLQAIERDGELRLLAPN
                     RYVIEWLGQNSLPRIKELISAFAEDPAPSLVLDVGTRSGVPSQVPVVNGGHAAANAVT
                     ARARRPGPSVLGGPINSGFTFDSFVEGKSNQLAKAAAIQVAGNPGRAYNPLFIYGGVG
                     LGKTHLMHAVANKIKENNAHARLAYVHSERFVGDMVKSLQHNTINDFKTAYRSLDALM
                     IDDIQFFAGKERSQEEFFHTFNALLESQQQVILTCDRYPKEVDGLEERLKSRFGWGLT
                     VAIEPPELETCVAILMSKAAISQVDLPEEVAFFIAKRIRSNVRELEGALRRVVATSNF
                     LGQPITLELVKEALRDLLALQEKLVTVENIQKTVAEYYKIRIADLLSKRRSRSIARPR
                     QVAMALAKELTNHSLPEIGDAFGGRDHTTVLHAVRRVKELRETDRRIGEDCMNLLRTL
                     AG"
     gene            10350..>10698
                     /locus_tag="VGE92_06160"
     CDS             10350..>10698
                     /locus_tag="VGE92_06160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF012915.4"
                     /note="binds the polymerase to DNA and acts as a sliding
                     clamp; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEY1313437.1"
                     /translation="MKLTAEREKLLAPLQAVIGVVERRQTMPILANVLLGVRQGQLSI
                     TATDLEVELVASTEVAVQEAGDITVPGRKFLDILRALPEKVSVSLNVEGEKVVIKAGR
                     SRFSLSTLPAAEFP"
BASE COUNT         2016 a         3207 c         3418 g         2057 t
ORIGIN      
        1 gagcgagacg cggcgcgcac tggcatcggc gctccaggtt actttcggcc acagatcgag
       61 attcggcggc tcgagcaggg cgggccccga gccatcgagc gtgaaatgga tatggcgcgt
      121 cgcggcgcaa ttcggaatga cggccacggg caacgaggcg gcatgcgtcg gatagtccaa
      181 gactttcacg tcgagtaccg tggtcaaacc gcccaggcct tgagcgccga tgccgagcgc
      241 gttgacgcgc tggaaaatgt cgagccgcag ctcttccaat ttgctgctcg gtccgcgcgc
      301 caagagctcg ccgatgtcga tgggctccat caaggatttc ttcgcgagca gcatggcctt
      361 ctcggcgctg ccgccgatgc cgacaccgag cattccgggc gggcaccagc cggcgcccat
      421 ttcaggcacc ctctcgagca cccagtcggc gacggagtcc gacggattca gcatggcgaa
      481 catggatttg ttttccgagc cgccgccctt cgctgcgacg gtaatctcga cggcatcgcc
      541 cgcgacgaat tccgtatgaa tcaccgcggg cgtattgtcg cgggtattcc cgcgtttgaa
      601 cgccgggtcc gacacgatgg atgcgcgcag cttgttgtcg ggattgaggt aggcgcgccg
      661 cacgccttcg ttgaccatcg cctcgagatc caggctcgcg tcccagcgca cacccatccc
      721 gactttcacg aataccacca cgatgccggt gtcctgacag attggacgat gaccctcggc
      781 gcacatgcgt gaattcgtga gtatctgcgc gatcgcatcg cgcgccgccg ggctttgctc
      841 gatctgatag gcatgcccca tggcgcggat gaaatccggc gtgtggtagt aggaaatgta
      901 ttgcagggca tcggccacgc tggcgatgac atcggcttcg cgaatcacac tcatggcaca
      961 accttacggt aatgacgaac tgggccgcaa atctaccaac tcacggacgg ctttgttgac
     1021 gaatccccta caatcggcag aaatcggaga ttcgaaatgc gagtgtggag agcgttgccg
     1081 tcgagcgccc ggggctgcgc cttgtggagt ttcctgggca ttgtcgggct gtggcctgcc
     1141 ggttgggcgg ccgccgccgc gccgctggcg ttgcacgagt gccgcatcga acatccgctg
     1201 cgcctgacat cgattgcggc ccgttgcgcc aacttgcgcg tgccggagaa tcgctccgag
     1261 cccggcggcg ccacgatcga gctcagcatc gcggtggtgc cggcgctcaa ccgccgcgcc
     1321 gccgccgcac ccttgttctt ggtcgccggc ggtccaggcc aaggaacgaa ggccatgtac
     1381 gcgggcctcg cgggcgcctt tgcgcgcgtc aatcgcgatc acgatatcgt attggtcgat
     1441 caacgcggca cgggagactc gacgcccctg tcctgcgact atcccgacga ttggcaggcg
     1501 caggacgaga tgctgcaggc gctgcgccag gcgaccacgg cttgtctgca aaagttcggc
     1561 gcgcgcgtgc gcttcttcac cagcggcgcg gcggttgcgg atctcgaggc ggtacgcacg
     1621 gcactcggct attcggcgct ggatctgtat ggcgtgtcct atggcacgcg cgtcgcccag
     1681 ctgtacatgc ggcgctatcc gcgcgccgtc cacgcagcga tcttggatgg agtgctcgct
     1741 cccgagcaag tcatcggacc ggccacaccg ctcgacggcg agcgcgcgct cgatctcatc
     1801 gtcgcgcgct gcaaagatgc gcccgaatgc gctcgagctt acccccagct gccgcacgac
     1861 ttggacgact tgcgcagccg atacggcatg caaaaatcca cgatcacgct ggatgatccg
     1921 gacggcggtc tgccgttcca attgcaattc aatcgcagtg tgttgaatgc cagcctgcgg
     1981 cttctctctt acagcgcgtc tcaagcgtcg ctcttgccga cgctcgtgca tcgcgcatcg
     2041 caaggcagct tgggaccgct cgcggcgcaa accgtcttga cggcgcgtca agcggcggca
     2101 cagattgcga tcggcatgca gaacagcgtg gtctgcagtg aggatgcgcc tttttttgca
     2161 ctctcggatg ccgcgcgggc ggccattgcc cgcacctacc agggaacaga tcagttggac
     2221 gcctttctgg aagtttgtaa attgtggccg cgcggggccg tcgatgccga tcttcacagt
     2281 ccgttacgca gcgacattcc gaccctgttg ttgtcgggcg aagcggatcc cgtgacaccg
     2341 ccggcggatg cggaacgcgc ggcgcagggg ctcgcgcacc atcgccactt gatattgagc
     2401 ggcgagggac acggtcaggt cggcaccggc tgcgtaccgc gattgatggc ggagtttctc
     2461 gaggcggcgg cccccgagaa actcgacgcg agttgcctga agcagcaccg tcccgcagcg
     2521 ttcttcgtca gcatgacggg cccggcgcca tgatcgaagc ttctcacttg agcaagcgct
     2581 tcggcgccat cgaagccgtg cgcgatgtgt ccttcagcgc ccgcgacggt gaaatcaccg
     2641 gcctgttggg cgccaacggt gcaggtaagt cgacctgctt gcgcatgctg tacggggtgc
     2701 tgacgccgga cagcggccgc gcatcaatca acggcatcga catccgcggc gagacctcga
     2761 aggcacgcgc gcagttgggc gtgctgccgc acgccgccgg tctgtacggc aatctcacgg
     2821 cgcgcgagaa catttacttt ttcggttcgc tgcagggaat cggtcgtgcg gccttgaagt
     2881 cgcgcaccac ggagctcgcg cgcgctctcg gcatggaaga attcatcgat cggcgcgcca
     2941 aaggcttctc gcaaggtcaa cgcatcaaga ccgctctggc ccgtgcgttg gtgcatgatc
     3001 ccggccacat cgtgctcgac gaacccacca atggactcga tgtgatggcg attcgaaact
     3061 tgcgcgagat gctcaaggcg ttgaaggctc aaggccgttg cgtcctgttc tcatcgcacg
     3121 tgatgcaaga ggtggctgcg ctgtgcgatc gggtggtgat cattggtcac ggccgagttc
     3181 tggcggatgc gagcgtgcaa tcgattcgcg aggacagcgg tgcggcctct ttggaggagg
     3241 cgttcctcaa ggtgctcggc gacagccacg gggcgagctg aatgcgcgcg ctgctcacgg
     3301 tcttccgcaa ggaatttctg gaaaacttgc gtgatcgacg caccgtcgtg tcggcgctgc
     3361 tgttcgggcc gttgttcggg cccttgatct tcggtctcat ggtgtcgcgg atgctcaatc
     3421 agagcttggt cgagtcgaac gagccgttgc gtctcacgat atcgggcggc gaacatgcac
     3481 cgggactgat gcgctatctc gaggcgcaag gcgtcgagct cgctacggca aaactatcgg
     3541 agagcgaggc caaggctgtc gtccgcggcg gcagcgcgaa agtagtgctg atcatccccg
     3601 cggaatacgc cgcgcgcttc gcggcggcgg cgccggcgcc ggtgctgctg gttgccgata
     3661 gcgcggattc gcagacccgc aagaccgcgg agcgcgcgcg actctttctc ggggcttaca
     3721 cgagcacgat ctcgcagctg cggctgcaaa tccgcggcgt gaatccgctg ctggcgctgc
     3781 cggtggcggt caatgacctc gatgtcgcga cgccgagcgg ccgcgccgtg gtggtgctcg
     3841 gcttcatgac ttatttcgtg ctgttcgcgg tactgatggg cggcctgtat cttgcgattg
     3901 actcgaccgc gggcgagcgc gagcgcggtt ccttggaggc gctgctgagt ttaccggtcg
     3961 cgcgctcgag cctggtggga gggaagatct tggccacctg cgcctacatg tgcatgtcgc
     4021 tcgccttgag cctcacggct ttcgtctgcg tgtttcgctt cgtgccgctg gagagcttgg
     4081 ggatgagcgc gaatttgggc gcgggcacgg cgctgatttt ctttgcaata tgtctaccgt
     4141 tcgtgccgct gggcgccgcg ctcatgacct ttgtagcctc gttcaccaag agctaccgtg
     4201 aagcccagac ctatctcgcg agcgtgctct tggtaccgac cctgccgatc gcctttgcca
     4261 gcatttattc gctgaaaacc cgcggctcgt tgatgttcat cccctcgctg agccagcact
     4321 tgctcatgac cagcgtgctg aaagatgagc cggtggcggc gctcgacgtg tgggtctcgg
     4381 ccggcgcgtc gctggcgttg gcgctgctgc tgatcgtgct gaccgcgcgt cattggcggc
     4441 gcgagaccat gctcggatag tttccataac gttaggggtc tttactatat ggtcgtttaa
     4501 caacaagacc tatatactgt tgccaggatt gcacatccgg ggggactggc atgaccgacg
     4561 cggcacacgc gcgcagcaat attgctgcac ttttgacaat cttggtgtta tcggccgcaa
     4621 ccatggtttg gttgttctgg cattttccgg tgattaccgc cgttgccacg ctcgccatcc
     4681 tcacgagtct cggcgtctcg gcgcgcctgg cgcgcttgac cgacagcgag gcgaaggatt
     4741 tgagccgcaa tcagcagggc gtgtgacgcg cgccttattt tccgatacaa aagctgccga
     4801 atatctcgcc cagcaaatcc tcactggtga actcaccggt gatctcgccc aaggcgtgct
     4861 gcgcgaggcg caagtcctcg gcgaatagct cgaacgcccg cgtgctgctc aatgtgttcg
     4921 cggcgctttg cacatgctgc cgggcgcgct gcaaggcgtt gagatgccgg cgccgcgcag
     4981 ccagggctcc cgattcgctc ggggtgtatc cggcactcgt ctgcaagtga gcgcgcagca
     5041 gatccatacc ggtaccaaag cgggcgctca ggaaaactcg cggcggcaca gaggcctcat
     5101 ccacccgcgc cggcagaccg gcaagatcga ttttgttgat aaccagggtc acggggatgc
     5161 cggcgggcaa gctcgcgagc tctgagcccg ccaaacccag ctgatcggtg atgccggcat
     5221 cgacgatgta cagcacgcga tcggcgcgcg cgatttcgct cctcgcgcgc ttcactccct
     5281 cggcctcgac gacatcgtcg acgctacgca gtcccgccgt gtcgatcagg ttcaccggca
     5341 ggccgtcgag atggacttgg caacgcaaga catcgcgcgt cgtaccgggc acctcggtca
     5401 cgatggctac ctcgtctccg gcgagccggt tcatcaggct ggattttccg gcgttgggct
     5461 tgccggcaat gacgacattc agaccctcgc gcagcagcgc gccttgcgcg gccgcggcgc
     5521 cgaccgatgc gaagccggca aacaccgcgg cgagccgctc gcgcaaagcc gcatcggata
     5581 aaaaatcgat ctcctcatcg ggaaaatcga tggccgcctc gacataggct cgtagcgaag
     5641 tgatcccgac attcaacgct gcgacccgcg ccgagaactc cccttgcatg gagcgcacag
     5701 cggcgcgggc ggcggccgcg cttccggctt cgatcaggtc ggcaatggct tcggcctgcg
     5761 cgatgtcgat cttgccgttc aagaaggcgc gttcgctgaa ctctccgggt cgggccaggc
     5821 ggcagccgag ctcgagcagg cgttgcaaaa ccaaatcgag aacgaccacg cctccatgtc
     5881 cttggatctc caagacgggt tcgccggtgt aggacgccgg cgccgggaaa tacaacgcga
     5941 gaccttcgtc gatgctggcg ttgtgcgcat cgaggaatct cgcgagccgc gcgcgccgcg
     6001 gattcggcaa ctcgccgaga atcccgacgg cgatctgcgg cacttgaggt cccgacacgc
     6061 gaatgacgcc gacggcgccg cgccccggag ctgtggcaat ggcggcgatg gtatccggcg
     6121 gcgtcacggc accttaactt cgaggcttgg cggcctcggc ttcaaccacc tgattcacgc
     6181 gccactgctg cagaatggtg aggagcgtat tggtcagcca gtaaagcacc aggccggccg
     6241 gaaaccacgc catcatgccc gtcatcacca gcggcatgaa ctgcatgatt tttgcctgca
     6301 tgggatcggg cggcgccgga ttcaacttga actgcgcgaa catcgcgccg cccatcaaca
     6361 agggcagcac gaagtagggg tcgcgcgaag aaagatcgtt gacccacatg agaaacggcg
     6421 cctggcgcat ctccacgctc tcgagcagca cccggtagaa agagatgaaa aacggaatct
     6481 gcaccagcat cggcaggcaa ccggcgagcg ggttgatttt ctccttcttg tacagctcca
     6541 tcatggctcg acctaatttc tcgcggtcgt ccttgaatgt gtcctggatc tgcttcatgc
     6601 gcggcgcgag ggtgcgcatc ttggccatgg aacggccgct cgcctggctc aagggataga
     6661 aaatcaactt gatgatggcc gtgacgaaga tgatcgacca gccccaattg ccgatgagcc
     6721 ggtgaaccca gttcagtccc aggaacagcg gttgcgcgag tacggtcaga attccgaaat
     6781 cgaccgtgcg ctccaaattc ggagcggttg ccgcgagctg cgattgcaat ttcggaccga
     6841 cgaacaattt ctcgcggaat tgcgcctggg cacccgcggc gacttcgctg accgggccgg
     6901 tggcgctcag caggtattcc ttgtcgcgca cctgcagctg gtacttgtac ggttgatcgg
     6961 ggggcggcac gatcgccgag acgaactggt gctgcagcga ggcgagccat ccgttggtaa
     7021 tcgtctcgga gaacttgctg tcggagtcgc tctccacgtt gaggtcatgg gattttctgc
     7081 cgtcccacac cgcgggccct ttgaacgagt aggtctcgac gtcgaaatag gagcgcgatg
     7141 catgctccca gtggcgcaga aattgcgaat acgaggcgag cttgcgcggc gccgtgccgc
     7201 cattttgaac atcgtaaatc aagtcgatgg aatacagccc gcgcgtgaaa acgaaagtct
     7261 tggtgacggt gagcccctgg ccgtcggtga aggtcagcgg cacgcgcagc tcttgcgcgc
     7321 ctgcggccag ctcgaaggat tgcgctgccg aggtgaaggt cgccagatgc gtcggcgcgg
     7381 cctgaccggc gtcgcccgtc aagccggtct gcagcaaata cagcgtgttc ggcggctcgc
     7441 ggctcaacag gcgcaccggg atattcggcg tgtccttgcg caagggatat tgcttcagat
     7501 cggcctgatc gagctcgccg cccttgaggt tgatcacgac gtcgagcacg tcggtggtga
     7561 catgcaccgg ctgcatcggg cccgcatcgg gggctgcagc cgggactacg cccggggctg
     7621 cgccgccgag cggcggcggt tcggcggccg gcggcacggc aaccgcgggt gcggccggcg
     7681 ttaccgcact cgccgcctgc ggcaccgaat cgccgagggt gctgacggga gcacccgtcg
     7741 tgctggtcgc gacggagttc agcgacggcg gataatcgtg catccaaact tgatagcagt
     7801 agaacaggat cgtaccgagc gcaatccaca ggagcatgcg ggtattaggc attgatcgtt
     7861 ctcttgtccg gcacggggtc gtagccaccc gcattgaagg gatgacagcg cagcacgcgg
     7921 cgcagcccaa gccacacacc gcgcacggct ccgtggcgct cgatcgcggc atgtgcgtag
     7981 caggaacagc tcggatagaa acggcaacgc ggccccagca acgggctcaa ggcgagctgg
     8041 tagccgcgga tgacccacat caagagttgg ctcatacgca tcgttgcgaa atcgatttcc
     8101 aatgctgatc gagactcgca cgcaagtcgc acgccttggc ttggcgcgcc gcatcccgtg
     8161 cagagacggt gacatcgacc ggcggcagtg aatgttggtt caaacgaaag gactcgcgag
     8221 tgatgcgctt gatgcgattg cgggcgaccg ccgtgccgaa ggttcgcgtc gcgatgctca
     8281 gccccaaccg aggataggat tcatgattgg ctagcaccga taaactaaaa aatgcgtcgg
     8341 cgaattttct gccgcgccgc ttcacgtcgc gaaattcggc cggctggtgc aaacgcttgc
     8401 gcggctcgaa cgtcagcctc ttgataaaat tggggatcgt tagacagtcc tgaaaatggt
     8461 gtcagggaca gaggcgcttg cggcccttcg cgcgtcggcg ggcaagcaac cggcgcccgc
     8521 tcttcgtggc catgcgcgcg cgaaagccgt gcgtgcgggc gcgatgtaga ttactgggat
     8581 tgaaagtacg tttcatagcg caactcaaac aaaaaccacg gggcaaatca atgaattggg
     8641 agatttaagg aaggcggcaa gggtactgag cgagcatgga tgtgtcaata gacgcatcgc
     8701 cacgttggag ctagactcgc tgccctttct tgcagtttgc cccattgccc aaaatttgag
     8761 gagagcgctc ttgtcggagt cgctatggaa ccgctgtctg cgcgtgctcg agaatgaact
     8821 cccggagcaa cagttcaaca cgtgggtgcg tccgcttcag gcgatcgagc gggatgggga
     8881 attgcggctc ctggcgccca atcgctatgt gattgaatgg ttgggtcaga attcgttgcc
     8941 gcgcatcaaa gaactcatca gcgccttcgc cgaggatccg gcgccgtcgt tggtgctcga
     9001 tgtgggaaca cgcagtggcg tcccgtccca ggtgccggtg gtcaatggtg gccacgcagc
     9061 ggccaacgcg gtgaccgcca gggcccggcg gccggggcct tcggtgctcg gcggcccgat
     9121 taattcgggg tttacatttg acagtttcgt cgagggcaag agcaatcagc tcgccaaggc
     9181 ggctgcgatc caagtcgcgg gaaatccggg cagagcctat aacccgctgt tcatttacgg
     9241 cggcgtcggt ctcggcaaga cccatctcat gcatgcggtt gccaacaaga tcaaggaaaa
     9301 caatgcgcac gcacggctcg cctacgtgca cagcgaacgc ttcgtcggcg acatggtgaa
     9361 atccctgcag cacaacacca tcaatgattt caagaccgcc tatcgctcgc tcgatgccct
     9421 gatgatcgat gacattcagt tcttcgcggg caaggagcgc tcccaggaag aattttttca
     9481 taccttcaat gcgctgctgg aaagccagca acaggtcatc ctgacctgcg atcgctatcc
     9541 gaaggaagtc gacggactcg aagaacggtt gaagtcgcga tttggctggg gtctcacggt
     9601 cgcgatcgag ccgccggaac tcgaaacctg cgtcgccatt ctcatgagca aggcggcaat
     9661 atcgcaggtc gatctgccgg aagaagtggc gttttttatc gccaaacgaa tccgctcgaa
     9721 tgttcgcgaa ctcgaaggcg cgctgcgccg cgtggtcgcg acctcgaatt tcctgggtca
     9781 gccgatcacg ctcgaattgg tgaaggaagc gctgcgcgat ctattggcgc tccaagagaa
     9841 actcgtgacg gtggaaaata tccaaaagac cgtcgcggaa tactacaaaa tcagaattgc
     9901 cgatctgttg tcgaagcgtc gcagccgttc catcgcaagg ccgcgccagg ttgcgatggc
     9961 gttggcgaag gaattgacca atcacagtct gccggagatc ggcgacgcat tcggcggtcg
    10021 ggatcacacg acagtgctgc atgcggtgcg ccgtgtcaaa gagctgcgcg agacggatcg
    10081 gcggattggc gaggactgta tgaacctgtt gagaaccctg gctggatagg gcggcagaag
    10141 ctgtggatga aactgtggat aaaagtatcc acaggtcgat gacagcttgc gagcaagccc
    10201 ccgacaggtg agcggtaggc tttggatgtt tatagaaggc tgattattag cgtgttttta
    10261 tagttatacc gaaaattcga gtacttcata atcatagtaa taatcttttt caagaatcta
    10321 aatctaagat tggtgagacg ggtgtgccca tgaaattgac ggctgaacgc gaaaaattgc
    10381 tggctccact gcaggccgta attggtgtgg tggagcggcg ccaaacgatg ccgatcctcg
    10441 ccaatgtatt gctcggcgtg cgccaggggc aattgtcgat cacggccacg gatctcgagg
    10501 tggagctcgt cgcgtccacg gaagtcgcgg ttcaggaggc gggtgatatc accgtcccgg
    10561 gccgcaagtt cttggatatt ttgcgcgcgt tgcctgaaaa ggtctcggtg tcgctcaacg
    10621 tcgaggggga aaaggtcgtc atcaaggcgg ggcgcagccg cttcagtttg tcgacgctgc
    10681 cggctgcgga gtttccgg
//
LOCUS       DASWZT010000222         3720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_83879, whole genome shotgun sequence.
ACCESSION   DASWZT010000222 DASWZT010000000
VERSION     DASWZT010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83879"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VGE92_06165"
     CDS             complement(<1..606)
                     /locus_tag="VGE92_06165"
                     /inference="COORDINATES: protein motif:HMM:NF040245.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313438.1"
                     /translation="MSSKSHDVEALLAQKYRHGFVTDIESDSLPPGLDESVVAAISKK
                     KNEPRFLLEWRLKAFRHWQTMQEPQWAHVHYKPIDYQDISYYSAPRAPANAPKSLDEV
                     DPKLLATYAKLGIPLHERARLAGVAVDAVFDSVSVATTFKEKLGKAGVIFCSFSDAVR
                     EHPGLIERYLGSVVPPGDNFFAALNSAVFSDGSFVFVPPGVR"
     gene            complement(624..1079)
                     /locus_tag="VGE92_06170"
     CDS             complement(624..1079)
                     /locus_tag="VGE92_06170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HEY1313439.1"
                     /translation="MLRMGKLTDYATVVLASLAQEPARHRAAAELAERTRLSRPTVSK
                     VLKGLQRAGMVISSRGAQGGYRLARNPEQITAAQILDIFEGPIAITECSGASSQCGIE
                     RQCRVGGAWQRVNAAIRRALEEVTLRQLAGLDATTMRVADLASEIRVNP"
     gene            1225..2247
                     /locus_tag="VGE92_06175"
     CDS             1225..2247
                     /locus_tag="VGE92_06175"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF012496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEY1313440.1"
                     /translation="MNTVELNATARAMVAKNRGLLAADESTSTIKKRFDTIKLESTEE
                     NRRTYREMLFTAPGAAEYISGVILYDETIRQKTKDGVPFPAYLAQHGMVPGIKVDMGA
                     KPLAAFPGETITEGLDGLRERLAEYYKLGARFAKWRAVIDIGSGIPSRYAIDANAQAL
                     ARYAALCQEAGITPIVEPEVLMDGAHTLERCEEVTNVVLDRVFEQLFAARISLEGMVL
                     KPNMVVSGKKCAQQATPEQVAEATLRTLKRQVPSAVPGIAFLSGGQSPTEATLHLSLM
                     NAAGPLPWALTFSYGRALQENALNSWGGKPAGYAGGQKALFIRAKLNGLAAIGAYKGS
                     MESSAA"
     gene            2309..3124
                     /locus_tag="VGE92_06180"
     CDS             2309..3124
                     /locus_tag="VGE92_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004809856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1313441.1"
                     /translation="MNQPAPIVDIRNVNYAVNGRPIFSGLDMQVPRGRITAVMGPSGT
                     GKTTLLRLITGQVIADTGSIVVAGQELLGVKRADLYVLRRRMGMLFQNGALLTDLSVF
                     ENVAFPLREHTDLPERLIRELVLTKLQAVGLRGAAELAPAELSGGMSRRVALARAIVM
                     DPEILIYDEPFVGLDPISLGVILRLIRRLNDALGITSIVVSHDVQEISTVADLVFLLS
                     GGKVAAKGTPQELQSYPSEIVRQFVGGLADGPVPFHFPAPDYFGELLARDEVT"
     gene            3121..>3720
                     /locus_tag="VGE92_06185"
     CDS             3121..>3720
                     /locus_tag="VGE92_06185"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1313442.1"
                     /translation="MKLFEELWASLLESLIGLGAVVRFLLRVTLELPRALLRPDLIAQ
                     QVYNSGALSLVIIMLSGLFVGMVLGLQGFQLLQRFGSEEALGTAATIGLVKELGPVVT
                     ALLFAGRAGTALASELGLMRATDQLSAMEMMAVDPIRRVVVPRFLGGVIAMPLLAAME
                     MMAVDPVRRVVAPRFLGGVIAMPLLSAIFNCIGIYGSQLV"
BASE COUNT          676 a         1143 c         1191 g          710 t
ORIGIN      
        1 gcgcacgccc ggcggcacga acacgaacga gccatcggag aacaccgccg aattcaaggc
       61 ggcgaagaag ttgtcgcccg gcggcacgac gctgccgaga tagcgctcga tgagtcccgg
      121 atgctcgcgc accgcgtccg agaacgaaca aaagatgacg ccggccttgc cgagtttctc
      181 cttgaaggtg gtggcaacgg agacgctgtc gaataccgca tcgactgcga cgccggcgag
      241 gcgcgcgcgc tcatgcagcg gaatgcccaa tttggcgtac gtcgcgagca gcttcggatc
      301 gacctcatcg aggctcttcg gggcattcgc cggcgcacgc ggcgcagaat aataggagat
      361 atcctggtag tcgattggct tgtagtgcac atgagcccac tgcggctcct gcatcgtctg
      421 ccagtgtcga aacgccttca ggcgccactc gagcaagaag cgcggctcgt ttttcttctt
      481 cgaaatcgcg gccacgacgc tctcatcgag tccgggcggc aggctgtcgg attcgatatc
      541 ggtgacgaag ccatgccgat acttctgggc gaggagcgct tctacatcgt gagacttgct
      601 ggacactggc atttcccgcc gcttcagggg ttgactcgaa tttccgacgc aagatcggca
      661 acgcgcatgg tggtggcgtc caagcccgcc aattgccgca gcgtcacctc ttcgagcgcg
      721 cggcgaatcg ccgcattgac gcgctgccag gcaccgccga cgcggcattg acgctcgatg
      781 ccgcattggc tggatgcgcc gctgcactcg gtgatcgcga tcggaccttc gaagatgtcg
      841 agaatctgcg ctgcggtaat ctgttcgggg ttgcgcgcca gacgatagcc gccttgcgca
      901 ccgcgcgagg agatcaccat cccggcgcgc tgcaatccct tcagcacctt gctcacggtc
      961 ggtcggctca ggcgcgtccg ctccgcaagt tccgccgccg cccggtggcg cgcgggctct
     1021 tgcgctagac ttgccaaaac gacggtcgcg tagtcggtca attttcccat tcgcagcata
     1081 taaacaccaa gcctcgggtc gaagcgttat gtgggactat tttagtccta tttcaattca
     1141 aggtccaccc gcaaggcggc ttcgggctta ttttgctatc cttacgcgct tcgacagggc
     1201 ccatagcagc gagacacgcc agacatgaac acggtagaac tcaacgccac tgcgcgcgcg
     1261 atggtagcga aaaacagggg tcttttggct gccgacgaga gcaccagcac catcaagaaa
     1321 cgcttcgaca ccatcaagct cgagtcgacc gaggagaatc ggcgcaccta tcgcgaaatg
     1381 ttgtttaccg ctccgggcgc cgcggaatac atcagcggcg tcatcctcta cgatgagacc
     1441 atccggcaga aaaccaagga tggggtgcca ttcccggcct acctcgctca gcacggcatg
     1501 gtgcccggca tcaaggtcga tatgggtgcc aaaccgctcg cagcttttcc cggcgaaacc
     1561 attaccgagg gactggacgg attgcgcgag cgcctcgccg agtattacaa actcggagcg
     1621 cgtttcgcca agtggcgcgc agtgatcgac atcggctcgg gaatccccag ccgctacgcc
     1681 atcgatgcca atgcgcaggc gctggcgcgc tacgcggcgc tgtgtcagga ggcggggatc
     1741 acgcccatcg tcgaacccga ggtcctgatg gacggtgcgc acacgctcga gcgctgcgaa
     1801 gaagtcacca atgtggtgct cgaccgggtg ttcgagcaat tattcgcggc gcgcatcagt
     1861 ctcgaaggca tggtgctcaa accaaacatg gtcgtgtcgg gcaaaaagtg tgcgcagcag
     1921 gcgacgcccg aacaggtcgc ggaagcgacg ttgcgcacgc tcaagcggca ggtaccgtca
     1981 gcggtgccgg gcattgcctt cttgtcgggc ggccaaagcc cgaccgaagc gactttgcac
     2041 ttgagcctga tgaacgcggc cgggccattg ccgtgggcgc tgaccttcag ctacgggcgc
     2101 gcgctgcagg agaacgcgct gaactcgtgg ggcggcaagc cggccggtta cgcgggcggt
     2161 cagaaggcgc tgttcatccg cgcgaagctc aacggtttgg cagccatcgg agcttacaag
     2221 ggctcgatgg aaagttccgc ggcttgacgt atcgcgctta actacagcgg ccaagcgtcg
     2281 tcgcgacgcc ggcgctacgg ccaagctatt gaaccaaccc gcacccattg tcgacattcg
     2341 caacgtaaat tacgcggtta acggccgtcc aatttttagc ggcctcgata tgcaggttcc
     2401 acgcggccgc atcacggccg tcatgggtcc gagcggcacc ggcaaaacca cgctgctgcg
     2461 tctcattacc ggtcaagtga tcgccgacac gggatcgatc gtggttgccg gccaagagtt
     2521 gctgggcgtg aagcgcgcgg atctgtacgt gctgcgccgt cgcatgggaa tgctgtttca
     2581 gaacggagca ctgctaacgg atctcagcgt gttcgagaac gtcgcctttc cgttgcgcga
     2641 gcacaccgat ttgccggagc gcctgatacg cgagctggtg ctcaccaaac tgcaggccgt
     2701 gggtttgcgc ggtgcagcgg aacttgcgcc ggcggagttg tcgggcggca tgagccgccg
     2761 cgtggcgctg gcgcgcgcca tcgtcatgga tcccgaaatc ctgatttacg acgagccctt
     2821 cgttggtctc gatccgattt cgctcggcgt cattctgcgc ttgatccggc gactgaatga
     2881 cgcgctcggc atcaccagca tcgtggtgtc gcacgacgtg caggagatct ccacggtcgc
     2941 cgacctggtg ttcttgctgt ccggcggcaa agtggcggcg aagggtacgc cgcaggagct
     3001 gcaatcctac ccctcggaaa tcgtgcggca attcgtcggc gggctggccg atggcccggt
     3061 gccattccat ttcccggcgc ccgactattt cggcgaactg ctcgcgcgcg acgaggttac
     3121 gtgaaactct tcgaggaact gtgggccagt ctgctcgagt ccctgatcgg gctgggcgcg
     3181 gtggtgcgtt tcctgctgcg tgtcacgctc gaattaccgc gtgcgctgct gcggcccgat
     3241 ctgattgcgc aacaggtgta caactccggc gcgctgtcgc tggtcatcat catgctctcc
     3301 ggcctgttcg tcggcatggt gctcgggctg cagggatttc agctgctgca gcgcttcggt
     3361 tccgaagagg cgctcggcac ggcggcgacg atcggattgg tgaaggaatt gggaccggtg
     3421 gtgacggcgc tgctgtttgc cggccgcgcc ggcacggcgt tggcctcgga attgggattg
     3481 atgcgcgcca cggatcaact atcggccatg gagatgatgg cggtcgatcc gatccggcgg
     3541 gttgtcgtgc cgcggtttct cggcggcgtg attgcgatgc cgttactcgc cgcgatggaa
     3601 atgatggcgg tcgaccccgt acgccgggtg gtcgcaccac gcttcctcgg cggcgtgatc
     3661 gcgatgccgt tgctcagcgc gatcttcaac tgcatcggca tctacggctc gcagctggtg
//
LOCUS       DASWZT010000223         4997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_45293, whole genome shotgun sequence.
ACCESSION   DASWZT010000223 DASWZT010000000
VERSION     DASWZT010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45293"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1692)
                     /locus_tag="VGE92_06190"
     CDS             complement(<1..1692)
                     /locus_tag="VGE92_06190"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEY1313443.1"
                     /translation="MTVDVVLAPRNQTDLDGMLTSVYDPKRPNYQQWLLKGEFYSRFA
                     PSADQIAAVAAYLQSSGLTVEQSSSPFMVRASGPSSAVEAALATTLHNYRNSKGITYF
                     ANASAIKLPAHLAAGVLGVVGATNTVRVHAHPKRAPNNAGSPPALACEAPYPTTTELF
                     LGTFLSGFGYGDAPLCSGLTPSQDNAIYGAPHLGNVAKGHGVNLALFELSAYQHSDID
                     YWAQQFYGPRYTPPLVDVDVDGGPLNPKCPKGDECVPGSEGYFGDVEVDADIEMSLAI
                     APAASRILVYNAPNDDTGQTELDEYTRIANDDVADVISSSWAGCENDSTAAYVQAENL
                     VFEQMALQGQSMFSAAGDTGAFECIRSDGTTLINVLDPAAQPWVTAVGGTSFFTFNPD
                     ANPNPSYPTGLESVWNPDGLCSNAGPSAANDEQGGFFWCANAGASGGGYSQFWGRPSY
                     QFGPGITNPYTAYANGTTHCALAKIGTPCREVPDVSANADGFTPYAEYCTPAGTETYS
                     GCAQPPIGWFGIGGTSLSSPFWSAIIADRDGFWHGRIGNANPLLYLLYNVDYQGYF"
     gene            complement(1870..2256)
                     /locus_tag="VGE92_06195"
     CDS             complement(1870..2256)
                     /locus_tag="VGE92_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY1313444.1"
                     /translation="MTAQLNHTIVYSSDRTRSARFLATILGRPAPVRFGPFEVVELDN
                     GVSLDFAAANDPIQPQHYAFLISEADFDAVFGRIRKQGIQYWADPRRHRPGEINHNDG
                     GRGVYFADPDGHYLEVITRPYGSGSS"
     gene            2447..>4997
                     /locus_tag="VGE92_06200"
     CDS             2447..>4997
                     /locus_tag="VGE92_06200"
                     /inference="COORDINATES: protein motif:HMM:NF023400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEY1313445.1"
                     /translation="MATSGGWGFRLRIVRLWRAWARMAQALLGTWQWDAPRWAIAAKS
                     AAVHGAAATVAIARRRPRSFAAGVMSVLVLMAAGAVLWHEYRHWPKPESAAFTVTPPE
                     RTRIERDGAKPEPLIVRFDRSVAPLALAGKPLLGGITLHPALAGTWRWLDDRVLEFRV
                     KDDWPVGQRFDVTLDKAVLAPQFRLESYTFEFAAASFAARLANAEFYQDPVNPGVKFA
                     VFDLEFTHPVDAAELERRVELRLAGQKEGVLGVGRETIGFNVVYDKWKLHASIHSASL
                     AVPKNPTTLELKVAAGVRAARGGNPSAAPLTASASVPGLYSLSIEGVEPMVVANARNE
                     PEQVLLLNVSAATGERDVACAVKAWVLPAVRAADAITPARGSPAAPYYWSDPQEVSEA
                     VLGQARPLALEPIAAEREFVESHSFRYRAEVGSFVLVQVDKNLRSFGGYLAARTERFI
                     VRVPPFPPALKILSQGAVLALSGDHKVAALVRDLPGVRVDIARVLPAQLQHWVSQSGG
                     DFANPTFTGNFGPDNLTERTSHKIPLGNLGHGQNHFETIDFSEYLKSGGSDKHGVFLL
                     TLRGYDPRVAPHDADDNDAMSDARLVLVTDLGIVLKRAADGTQDVFVQSIASGLPVAG
                     ASVDIIAKNGTTLLSHPTDADGRAHFLKMENLTRERTPLLVQVQKAGDFSFLPLNHAD
                     RNLDYSRFDVGGVHSARTATQLSAYLFCDRGIYRPGDTLHVGMIAKTALGSAELAGIP
                     LEAEILDARGLTALRQRFKLPGSGFTELSHTLPDTAPTGAYSVNLYIVKDDRPDAEIG
                     TTTLRVQEFEPDRMKVSAHLSAESLEGWVSPQDLKATVNVANLFGTAAQQRR"
BASE COUNT          910 a         1601 c         1588 g          898 t
ORIGIN      
        1 gaagtagccc tgataatcga cgttatacag aaggtagagc agcgggttcg cattcccgat
       61 ccggccatgc cagaagccgt cgcggtcagc aatgattgcc gaccagaaag gcgatgacag
      121 gctggtgccg ccaatgccga accagccgat gggcggctgg gcacaaccgc tgtaagtttc
      181 ggtcccggcc ggggtgcagt attccgcata gggagtgaac ccatccgcat tcgcagacac
      241 gtccggaacc tcgcggcacg gcgttccgat cttggctagc gcgcagtgcg tcgtaccgtt
      301 agcgtaggcc gtgtacgggt tcgtgatgcc cgggccgaac tggtacgagg gacggcccca
      361 gaactggctg tatccgcctc cactggcacc cgcgttagca caccagaaga accccccctg
      421 ctcatcattg gctgcactcg gacccgcgtt actgcagagg ccgtcgggat tccacacgct
      481 ctccaggcct gtcgggtacg aaggattcgg gttggcgtcc ggattgaatg tgaagaaaga
      541 cgtccctccc acggcagtca cccacggctg cgcggcggga tcgagcacgt tgatgagggt
      601 tgtgccatcg ctccggatgc actcgaaagc gcccgtgtcg cccgcggcac tgaacatgct
      661 ctgcccttgc agcgccattt gctcgaacac caggttctcg gcctgcacgt atgccgcagt
      721 cgagtcattc tcgcaaccgg cccagctgga gctgatgaca tccgcaacgt cgtcattcgc
      781 gatcctggtg tactcatcga gttcggtctg cccggtgtca tcattcggag cgttgtacac
      841 gaggatcctg ctcgcggcag gtgcaattgc cagcgacatc tcaatgtccg cgtcgacctc
      901 gacgtcccca aaatagcctt cagaaccggg gacacactca tcgccctttg ggcatttcgg
      961 gttcagcggg ccgccatcga catcgacgtc gaccagtggc ggtgtgtagc gcgggccgta
     1021 gaactgctgc gcccaatagt cgatgtcgga gtgctgatag gccgagagct cgaacagcgc
     1081 caggttgact ccgtggccct tcgcgacatt ccccagatgc ggcgcgccat agattgcgtt
     1141 gtcttgtgac ggcgtcagac cgctgcagag gggagcatcc ccgtaaccaa aaccagacag
     1201 aaacgttccg agaaagagct cggtcgtggt gggatacggc gcctcgcagg ctagggctgg
     1261 cggggaaccg gcgttgtttg gcgcacgctt tggatgcgcg tgcacacgta cggtattagt
     1321 agcgcccacg acgcccagca cgcctgcggc taggtgcgca ggtagtttga tggcagaagc
     1381 attcgcgaag taagtgatgc ctttcgaatt gcggtagttg tgcagtgtcg tggcgagtgc
     1441 cgcctcgacc gcgctgctgg gtccgcttgc gcgcaccatg aacggtgagg atgactgctc
     1501 gaccgtcaag ccactcgact gcagatacgc cgccacagcg gcaatctggt cagcgctggg
     1561 cgcgaagcgc gagtagaact ctcctttgag cagccactgc tgatagttcg gacgcttcgg
     1621 atcgtagaca ctcgtcaaca tcccatccag atccgtctgg ttgcgcggcg cgagcacaac
     1681 atcgacggtc atcctgtcgc tcctgtaccc acccaagtcg acatcgaccg tcggtttgaa
     1741 actccccgca agcacagata ggtcttcgct cgcgttggcc acagaccccg catatgtccg
     1801 attgtagacc cggtcaattg cgtgccgagc cgaacatcga ggccacaccg atgcagcctt
     1861 atcacctgct cagctactac cacttccgta cgggcgcgta atcacttcca agtagtggcc
     1921 gtcagggtcg gcaaagtaga ccccgcgtcc accgtcgtta tgattgattt cgccggggcg
     1981 atggcgcctc ggatcggccc aatactggat accctgcttg cggatgcggc cgaatacggc
     2041 atcgaaatcg gcctcggaaa tcaggaaagc gtagtgttgc ggctggatgg gatcattggc
     2101 ggctgcgaaa tcgagagaca cgccgttgtc gagttcgacc acttcaaatg gaccgaaccg
     2161 aactggcgcc ggtcgcccca gtatcgtcgc gaggaaccgg gcagacctcg tccggtcaga
     2221 cgagtaaacg atggtgtggt tcagttgagc ggtcatgtga ttggccctca tcactttcgg
     2281 cgaaaggtcg cggttgcaaa gatttatctt cgccccagta tacggcgacg ctcctgctga
     2341 ctcagtctcg ggccacaatt gctcactgca ttgccatgat tgctcacaac gcgccgcgca
     2401 cgcgctgcta gcgtaacccc gaggcgtgaa tcgccgggga ggcgtgatgg cgacatcagg
     2461 tgggtggggt tttcggctgc ggatcgtacg gctgtggcgc gcgtgggccc gcatggcgca
     2521 agcgctcttg ggcacatggc aatgggacgc accgcggtgg gcgatcgcgg ccaagtccgc
     2581 ggccgtgcac ggcgcggcgg cgaccgtggc gatcgcgcgc cgacggccgc gcagtttcgc
     2641 cgccggcgtg atgtcggtgc tggtgctcat ggccgccggc gcggtccttt ggcatgagta
     2701 tcggcattgg ccgaagcccg agtccgccgc cttcaccgtg acaccgcccg agcgcacgcg
     2761 catcgaacgc gatggcgcga agcccgagcc gctcatcgtg cgcttcgatc gctcggtcgc
     2821 gccgctcgcg ttggccggaa aacccctctt gggtggcatc acgctgcacc ccgcgctcgc
     2881 cggcacctgg cgctggctgg atgatcgcgt gctggaattc cgggtcaagg acgactggcc
     2941 ggtcggacag cgcttcgacg tgacgctcga caaggccgtg ctcgcgccgc agtttcgcct
     3001 cgagagctac acattcgaat tcgccgccgc gagcttcgcc gcacgcctcg cgaacgccga
     3061 gttctatcaa gacccggtca atccgggcgt caagttcgcg gtcttcgact tggagttcac
     3121 gcacccggtc gatgcggcag aactcgagcg ccgcgtcgaa ctgcgccttg ccggtcaaaa
     3181 ggaaggcgtg ctcggcgtcg gccgcgagac gatcggattc aacgtcgtct acgataaatg
     3241 gaagttgcat gcctcgatcc actcggcaag cttggccgtg cccaagaatc cgaccacgct
     3301 ggaactcaaa gtggcggccg gcgtgcgcgc cgcacggggc ggcaacccga gcgcggcgcc
     3361 gctgacggcg agtgccagcg tgcccggcct ttacagccta tcgatcgagg gcgtcgaacc
     3421 catggtcgtc gccaatgcgc gcaatgaacc cgaacaagtg ctgctcctca acgtgtcggc
     3481 ggccaccggc gagcgcgacg tggcttgcgc ggtgaaggcc tgggttctgc cggccgtgcg
     3541 cgccgcggac gcgatcacgc ccgcgcgcgg cagcccggcc gcgccttatt actggtccga
     3601 cccgcaggaa gtcagcgagg cggtgcttgg tcaagcgcgg ccgcttgcgc tcgagccgat
     3661 tgccgcggag cgcgaattcg tggaaagcca tagctttcgc tatcgcgcag aggtcggcag
     3721 cttcgtcctg gtgcaagtcg acaagaacct acgttccttc ggcggctatc tcgcggcgcg
     3781 caccgagcgc ttcatcgtgc gcgtgccgcc gtttccgccg gcgctcaaga ttctgtcgca
     3841 gggggcggtg ttggcgctct ccggtgatca caaggtggcc gcgctggtgc gcgacctgcc
     3901 tggcgtgcgc gtcgatattg cccgcgtgct gcccgcgcaa ctgcagcact gggtatcgca
     3961 gtccggcggc gatttcgcga accccacgtt cacaggcaac ttcggccccg acaatctcac
     4021 cgagcgtacg tcgcacaaaa ttcccttggg caacttaggc cacgggcaaa accatttcga
     4081 gaccatcgat ttcagcgagt acctaaagag cggcggcagc gacaagcacg gggtgtttct
     4141 gttgacgctg cgcggctatg atcctcgcgt cgcgccccac gatgccgatg acaacgacgc
     4201 catgagcgat gcgcgcctgg tgctggtcac ggatctcggc atcgtgctca agcgcgcggc
     4261 cgacggcacg caggatgtgt tcgtgcaatc gatcgcgagc ggactgccgg tcgcgggcgc
     4321 gagcgtcgac atcatcgcca agaacggcac cacgctgttg tcgcacccga cggatgcaga
     4381 cgggcgcgcg cattttctga aaatggaaaa cctcacgcgc gagcgcacac cgctcctggt
     4441 gcaagtccaa aaggccggcg acttcagttt cctgccgctc aaccatgccg accggaatct
     4501 cgactattcg cgcttcgacg tcggcggcgt gcatagcgcc cgcaccgcaa cccagctgag
     4561 cgcatacctg ttctgcgacc gcggcattta tcgtccgggc gacaccctgc atgtgggcat
     4621 gattgcgaag acagcgctcg ggtccgcgga actcgccggc attcccctcg aggcggaaat
     4681 cctcgacgcg cgcggcctca ccgcgctgcg acagcgcttc aagctgccgg gcagcggctt
     4741 caccgaattg tcgcacacgc tgcccgacac ggcgccgacg ggggcgtact cggtgaatct
     4801 gtacatcgtc aaggacgatc ggcccgacgc cgagatcggc acgaccacgc tgcgcgtgca
     4861 ggagttcgaa cccgaccgca tgaaagtgag cgcgcacttg agcgccgaga gcctcgaggg
     4921 ctgggtgtca ccgcaggact tgaaggcaac ggtgaacgtc gcgaacttgt tcggcacggc
     4981 agcgcagcag cggcgag
//
LOCUS       DASWZT010000224         2623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_35572, whole genome shotgun sequence.
ACCESSION   DASWZT010000224 DASWZT010000000
VERSION     DASWZT010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35572"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2623)
                     /locus_tag="VGE92_06205"
     CDS             complement(<1..>2623)
                     /locus_tag="VGE92_06205"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313446.1"
                     /translation="RINRKLRARRGARLAAITSGGAIPDNALFTVVADPDGGTVGTVD
                     EDFAVESMAGDVMLLGNTSWRIRRVEGKSSRMLVEDAHGQPPSVPFWRGEAPARTAEL
                     SQQLGELRKEISDRLPGVLPIEGWRNLPVVAETASWLEEQCGLDLSGAEQLLQYTLEG
                     RTVLGDVPTQTTIIAERFFDEGGGMQLIIHAPFGGRINKAWGLALRKRFCRGFNFELQ
                     AAATDNGLNIALAEQHSFPLADVFHFLHSETMAPILEQAALASPIFGTRWRWDATRAL
                     ALLRFQGGKKVPPQIQRMRSDDLLASVFPDVAACQENIEGDIKIPDHPLVHEVMKDVL
                     TEALDIEGLSEVLRGIEQGRIRCLAVDTPVPSQFSHEILNANPYAYLDDAPLEERRAR
                     AVEMRRVLPASVLEEIGALDPAAIAQVQQEAWPDVRDADELHDVLHTLVMVPASGQLP
                     ALSFQQHRMAAVWGNLFERLQGENRAVVAEAAGRRQYWVAAERAEWLKAMHPLARVMT
                     GLCPVPAGQSPATTQADAIRPISSPGFDDALLTAVQGWIAHLGPTTASEIGELLALPA
                     SEIEKALLRIEASGAILRGKFRPNSRPAAFESEPETEWCERRLLARIHRLTVATLRKQ
                     IAPVTASQFMNWLLRWQHLAPGTQVRGEHGTLEVIRQLQGFEIPASAWERFVLARRIT
                     DYDPALLDQLCLAGVVGWGRLSPHPATLDAVVNDENGREIRRRVIPTRVAPIAFFVRE
                     DADWLRPHHPGAEDSATSFLSPAAQSLLELLKHRGALFFPDMVRATGRLKAEIETGLW
                     ELVAAGLVTADGFENLRALVTPKGSSGSGLARGIGKARRPRHAPGRWSLLHTEGADRE
                     HSVESCCWMLLRRYGVVF"
BASE COUNT          515 a          872 c          762 g          474 t
ORIGIN      
        1 gaatacgact ccgtagcggc gcaacagcat ccagcagcag gattcgacgc tgtgctcgcg
       61 atcggcgcct tcggtatgca agagcgacca gcgtccgggc gcatggcgcg gacggcgggc
      121 ctttccaatc cctctcgcca gaccgctgcc agaggagcct ttcggcgtca ccagggcccg
      181 caggttttcg aatccgtctg cggtcaccaa ccccgccgcg accagttccc acaatccggt
      241 ttcgatttcg gctttgagac ggcctgtcgc tcgcaccatg tcaggaaaaa acaaagcgcc
      301 acggtgcttc aaaagttcga gcagcgattg cgccgccgga cttagaaacg aagtggcgga
      361 atcttccgca ccgggatgat ggggacggag ccaatcggca tcttcacgca caaagaacgc
      421 aatgggcgcc acccgcgtcg gaatcactcg ccgccggatc tcgcgcccat tttcatcgtt
      481 cacgacggcg tcaagcgttg ccggatgcgg cgacaggcgg ccccatccaa ccacgccggc
      541 gagacatagc tgatcgagaa gcgccggatc gtagtcggta attctgcggg caagcacaaa
      601 acgctcccac gcgcttgccg gaatttcaaa gccctgcaac tgccggatca cttcgagcgt
      661 tccgtgctcg ccccgcacct gggtgcccgg agccagatgc tgccagcgca aaagccaatt
      721 catgaactgt gacgcggtta ccggagcgat ctgtttgcgc aaggtcgcga ccgtcagccg
      781 atgaatgcgc gccaggagac gccgctcgca ccactcggtt tcaggttcgc tttcgaatgc
      841 cgctgggcgt gaattggggc gaaacttacc gcgcaaaatc gctccgctgg cctcgatgcg
      901 aagcagagct ttctcaatct cagacgccgg caaagcaagc aattcgccga tttccgatgc
      961 ggtcgtaggg ccaagatgcg cgatccatcc ctgcacggca gtcagcagag cgtcgtcaaa
     1021 accgggactc gatatggggc gaatcgcgtc ggcctgtgtg gtggcggggc tctgccccgc
     1081 tgggacgggg caaagccccg tcatcacacg tgccagagga tgcatcgcct taagccactc
     1141 tgcgcgctcc gcggcgaccc aatattgccg ccgccccgca gcttcggcca ccacggcacg
     1201 attctccccc tgcaacctct cgaagaggtt tccccaaacc gccgccatcc gatgttgctg
     1261 aaagctgaga gccggcagtt gaccgcttgc cggcaccatc accagcgtat gcagcacgtc
     1321 atgcagttca tcggcgtcgc gcacatccgg ccacgcctcc tgctgcacct gcgcgatcgc
     1381 cgctgggtcg agcgcgccaa tctcctcgag caccgacgcc ggcaataccc gccgcatctc
     1441 gaccgcccgc gcccggcgct cctcgagcgg cgcgtcgtca aggtacgcgt agggattcgc
     1501 attgagaatt tcgtgcgaga attgcgacgg cacgggagta tcgaccgcaa ggcaacgaat
     1561 ccttccctgt tcgattccgc gcaatacttc gctcagacct tcgatgtcga gcgcctccgt
     1621 gagcacatct ttcatgactt cgtgcaccag cggatgatcg ggaatcttga tgtcgccttc
     1681 aatgttttcc tggcacgccg ccacatcggg aaacaccgat gccagcagat cgtcggagcg
     1741 catgcgctga atctgcggcg gaactttctt gccgccctga aagcgcagca aagctagtgc
     1801 ccgcgtggcg tcccaccgcc agcgggtgcc aaagattggc gacgccagcg ccgcctgctc
     1861 caaaatcggc gccatggtct cggagtggag aaaatgaaaa acatcggcca acggaaagct
     1921 atgctgctcg gccagagcaa tgtttaatcc gttatctgtg gcggccgctt gcagttcgaa
     1981 attaaagccc cggcagaaac gcttgcgcaa ggccagcccc caggccttgt tgatgcgtcc
     2041 gccaaagggc gcgtggatga tcaactgcat cccgccgccc tcgtcgaaaa atctttcggc
     2101 gataatcgtc gtctgtgtcg gcacatcgcc gaggacggta cggccctcga gcgtgtattg
     2161 cagcaactgc tcagcgccgg agagatcgag accgcactgt tcttccagcc aactcgcggt
     2221 ctcggcaaca accggcagat ttcgccatcc ttcaatagga agcacgccgg gcagccggtc
     2281 gctgatctct ttgcgcaact cgccgagttg ttgcgaaagc tcggcagtgc gcgcgggagc
     2341 ttctccacgc cagaagggta cgctgggcgg ttggccgtgc gcatcttcga ccagcatgcg
     2401 cgacgacttc ccctccactc gccttatgcg ccatgaggtg ttgcccagga gcatgacgtc
     2461 tcccgccatg ctctctaccg cgaaatcctc atcgaccgtt ccaacggtgc cgccgtcggg
     2521 gtcggcgacc acggtaaaaa gagcattgtc gggaattgcg ccgccgctgg tgatcgcggc
     2581 cagccgcgct cctcgccgcg cccgcagctt gcgattgatg cgg
//
LOCUS       DASWZT010000225         5084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_40556, whole genome shotgun sequence.
ACCESSION   DASWZT010000225 DASWZT010000000
VERSION     DASWZT010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40556"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1479)
                     /locus_tag="VGE92_06210"
     CDS             complement(<1..1479)
                     /locus_tag="VGE92_06210"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY1313447.1"
                     /translation="MKFALGSIVGLLALVPLPGFADAPGPPQSYAIDQTRYFATPEIE
                     SAEFKQRMEEAAAFAEVAPDDPIALAAYLKHAESLLAQLERHNAYLHLRASRDIEDRA
                     DADAADRTSDAMDTLVDTVGAALRTLGSAAFAKDVETNPDLSRYAYLLARAERTRPHE
                     LPQDQARILHELADPAAANSWTLYQQTVRSTPFKKIVSAEGELDVKKDARLLAQNPDR
                     SVRQAAWAARWDGYVSRADIYANILFGIVRMNDRVARLQHFPDAPSKVYFSRDLDRKD
                     VEQALARIEKRADLLQSYQRSRARHIAAVAGITDVHPWDWSVPMPGFSVPRLTLEETR
                     AEVIGALAPLGADYVEHFRQLLDPANGRMDVAAQVGKRTNAGFSIGAPGVASGLFVES
                     YGPGLLGDTRVVIHEGGHAVHKQLMSESGVSPFYTRGPNWMFEAFAILNEFLLYDHLY
                     QSSADPKARVYYLEALIDNMAFQLFTSAEEASLEQAVYDGVVA"
     gene            complement(1493..2281)
                     /locus_tag="VGE92_06215"
     CDS             complement(1493..2281)
                     /locus_tag="VGE92_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY1313448.1"
                     /translation="MSQAASPTVRLEIREHIAHLTLARPEAANTMNLQFGREFFAAVR
                     AIEGAVQVRAVILTGEGKNFCFGGDLKGMIASGADVGAYLTELTTDLHAGIALLVRLD
                     APVIAAVNGTAAGAGLGLVLAADLAIAARSAKFAPAYTGVGLTPDAGCTFLLPFAVGH
                     KRAMDLFLTNRVLDAQQALDWGIINRVVDDESLAEAAAALATRLAAGPRGAFGAVKRL
                     VAEAQPGFVAQLARESASISSRGSSAEGREGISAFLDKRAPQFP"
     gene            complement(2278..3189)
                     /locus_tag="VGE92_06220"
     CDS             complement(2278..3189)
                     /locus_tag="VGE92_06220"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEY1313449.1"
                     /translation="MKIEIVEVGPRDGLQNESVLFSTPQKLELIGRALDAGVRRIEAA
                     SFVNPKLVPQMADAEAVSAGLPRREGVVFIGLVLNKRGALRAIESGMDELGAVCAASD
                     GFATRNQGMTSDASLAMCCDVVRLARAHSRRAQITISTAFGCPFDGEVDPSRVVEMAR
                     TAAQAGPVEVAVADTIGVASPGEVSTLIARVAAAIKPLPVRAHFHNTRNTGLANVWAA
                     VQAGVTTIDASLGGIGGCPFAPRATGNVPTEDVAYMLSRSGYDTGLDLERLIGSARWL
                     AAAMGRDVPGMLSRAGAFPFRLPESFS"
     gene            complement(3223..>5084)
                     /locus_tag="VGE92_06225"
     CDS             complement(3223..>5084)
                     /locus_tag="VGE92_06225"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY1313450.1"
                     /translation="GVPSAAASRAATADAASEFAASADATVSMYCLADDAPAGRRDAG
                     LYRVEALRSGAVIDGPAVLAERNATTIVEPGWRVTVSAAGCLDMQRVRARAARHAPGT
                     RVDPVRLELFNNLFMNIAEQMGLRLQNTAFSVNIKERLDFSCALFDAEGSLIANAPHM
                     PVHLGSMSESIRTVIAQNPAMQPGDVYVLNDPYHGGTHLPDVTVVTPVYLDAADLVPG
                     FFVASRGHHADIGGTTPGSMPPFSTSLAEEGVLIDNFKLVERGAWREAALRELLAAGP
                     HPSRNITQNLADLRAQIAANEKGAQELKSTVAQYGRPTVVAYMRHVQDNAEESVRRVI
                     TALKDGEFLLPLDNGANIRLSVRVDALRRSACVDFTGTSAQLPNNFNAPRAITTAAVL
                     YVFRTLVDDDIPLNAGCLKPLQIILPEGCMLNPRAPAAVVAGNVETSTCVTNALFGAL
                     RVMAASQCTMNNFTFGNARHQYYETIAGGSGAGPEFDGASVVQTHMTNSRLTDPEVLE
                     SRFPVRLESYEIRAGSGGRGRWRGGNGGVRRIRFLEPMTASILSNGRRHGAFGMSGGE
                     PGAVGENWIERRDGRIERLEHIGQADMQAGDLFVISTPGGGGYGPISQHNLHK"
BASE COUNT          941 a         1693 c         1570 g          880 t
ORIGIN      
        1 tgccaccacg ccgtcataca cggcttgctc gaggctggct tcctccgccg acgtgaagag
       61 ctgaaatgcc atgttgtcga tgagcgcttc gaggtagtac acccgcgcct tgggatccgc
      121 gctgctctga tacaggtgat cgtacagcag aaactcattc aaaatggcga atgcttcgaa
      181 catccagttc gggccgcggg tatagaaggg cgacacgccg ctctcactca tcagctgttt
      241 gtgaacggca tggccgcctt cgtggatgac gacacgcgtg tccccgagta ggccgggccc
      301 gtagctctcc acgaacaacc ccgacgccac gcccggcgcc ccaatcgaga agcccgcatt
      361 ggtgcgcttc cccacctgcg cggcgacatc catgcgccca ttggcaggat caagcagttg
      421 ccggaaatgt tccacgtaat ccgccccgag cggagccagc gcgccgatca cttctgcgcg
      481 agtctcctcg agggtgagcc gcggaacgct gaagccgggc atcggcaccg accagtccca
      541 gggatgcacg tcggtgatgc cggcaacggc cgcgatgtgc cgcgcccgcg agcgctgata
      601 gctttgcagc agatcggcgc gcttctcgat gcgcgccaga gcttgctcga cgtccttgcg
      661 atcgaggtcg cgggaaaaat acactttgct cggcgcatcg gggaagtgct gcagacgcgc
      721 cacgcggtcg ttcatccgca cgataccgaa caggatattg gcgtaaatgt ccgcccgcga
      781 cacgtaacca tcccatcgcg cagcccacgc ggcctggcgg accgatcggt ccgggttctg
      841 ggccagcagc cgcgcgtctt ttttgacgtc gagttcaccc tcggcggaca caatcttctt
      901 gaacggcgtg ctgcgcaccg tctgctgata gagggtccag gaattggcgg cggcgggatc
      961 ggcaagctca tgcagtatcc gcgcttgatc ctgcggaagc tcgtgcgggc gggttcgttc
     1021 ggcgcgcgcc agcaggtagg cgtatcggct caaatccgga ttcgtttcaa cgtccttcgc
     1081 gaacgcggcg ctgccgagcg tgcgcagcgc agcgcccacc gtgtccacca gagtgtccat
     1141 cgcgtcgctg gttcggtcag ccgcgtcggc atccgcccgg tcctcgatat ctcgggatgc
     1201 ccgtaggtgc agatacgcat tgtggcgctc gagttgcgcg agcagagatt cagcgtgctt
     1261 gagatatgcc gccagggcga tcggatcatc cggggcgacc tcggcaaacg ctgcggcctc
     1321 ctccatacgc tgcttaaatt cggcagactc gatctcggga gtcgcgaaat agcgcgtctg
     1381 atcgatggcg taggattgcg ggggcccggg cgcgtccgcg aaaccaggga gcggcacgag
     1441 ggcgagcaac ccgacgatcg aacccaatgc gaatttcacg gcgacggctg aatcacggaa
     1501 actgcggcgc gcgcttgtcg agaaatgcgc tgatcccctc gcggccctcg gcactcgagc
     1561 ctcgcgatga aattgaggcg ctctcgcgcg cgagctgggc cacaaagccc ggctgcgctt
     1621 ccgccacgag tcgtttgacc gccccgaagg cgccgcgcgg tccggcagcg aggcgcgtgg
     1681 cgagagcggc cgccgcctcc gcgagactct catcgtcgac cacccggttg atgattcccc
     1741 agtcgagcgc ctgttgtgca tcgagcactc gattggtcaa gaacaggtcc atcgcccgtt
     1801 tgtgacccac cgcgaacggc agcaaaaacg tgcagccggc atccggtgtg agaccgacgc
     1861 ccgtgtaggc gggcgcaaac ttcgccgagc gcgccgcgat tgcgaggtcc gcggccaata
     1921 ccaggccaag acccgccccc gccgcggtgc cgttgaccgc cgcgatcacc ggggcatcca
     1981 gtcgaaccag cagcgcgata cctgcgtgca agtccgtcgt gagttcggtc aaatacgcgc
     2041 cgacatcggc gcccgaggcg atcattcctt tcaggtcgcc gccgaaacag aagttctttc
     2101 cctcaccggt caagatgacc gcacgcactt gaacggcgcc ttcgatggcg cgtaccgcgg
     2161 caaagaactc gcggccaaat tgcaggttca tcgtgtttgc agcttcgggc cgcgccaacg
     2221 tcagatgagc aatgtgctcg cggatctcga gacggacggt cggcgaagcg gcttggctca
     2281 tgagaaggac tccggcaatc gaaatggaaa tgcgccggcg cggctcagca taccgggaac
     2341 atcgcgcccc atcgcagcgg caagccagcg cgccgagccg atgaggcgct ctaaatccaa
     2401 tcccgtgtcg tagcctgatc gactcagcat gtaggccaca tcctcggtgg gcacattgcc
     2461 ggtggcgcgc ggcgcaaacg gacagccacc gattccgccc aacgacgcat cgatggtggt
     2521 tacgccggct tggaccgcgg cccagacatt ggcaaggcct gtgtttcggg tattgtgaaa
     2581 gtgcgcgcgc accggcagcg gcttgatggc cgcggccacg cgcgcgatga gtgtcgatac
     2641 ctctccggga cttgcgacgc cgatcgtgtc cgctaccgcg acctccaccg ggcctgcctg
     2701 cgccgcagtg cgcgccatct ccaccacccg cgacggatcc acttcaccgt caaatggaca
     2761 gccaaacgcg gtcgaaatgg tgatttgcgc tcggcgcgag tgcgcgcgcg ccaagcgcac
     2821 cacatcgcaa cacatcgcga gcgacgcgtc ggaagtcatg ccttgattgc gagtcgcaaa
     2881 cccatcggac gccgcgcaca ccgcgccgag ttcgtccatg cccgactcaa tggcgcgcaa
     2941 cgcaccacgt ttgttcagca ccaggccgat gaataccaca ccctcgcggc gcggtaatcc
     3001 cgccgatacg gcttcggcat ccgccatttg cggtaccagt ttcgggttga cgaaactcgc
     3061 cgcctcgatt cggcgcacgc ccgcatcgag cgcgcggccg atcagctcga gcttttgcgg
     3121 ggtgctgaac agcaccgact cgttctgcag gccgtcgcgc ggaccaacct caactatttc
     3181 tattttcaca ataaattcgc tatttcaata acttactaac atttacttat gtaaattatg
     3241 ctgagaaatc ggcccataac cgccgccgcc gggcgtagag ataacaaata aatcgcccgc
     3301 ctgcatgtcg gcctgaccga tatgctccag gcgctcgatg cgaccgtccc gacgctcgat
     3361 ccaattctcg cccacggcgc cgggctctcc gccgctcatg ccgaacgcac cgtgacgccg
     3421 gccgttgctc aagatcgatg ccgtcatcgg ctccaggaag cggattcgcc gcactccacc
     3481 gttgccgcct cgccagcgcc cccgcccccc cgagcccgcc cgaatctcat aggactcgag
     3541 gcgtaccggg aagcgagact ccagcacctc cgggtccgtc aggcgcgagt tggtcatatg
     3601 tgtctggacc acgctggcac cgtcgaattc aggtcccgcg ccggagccgc cggcgatggt
     3661 ctcgtagtac tgatgccgag cattgccgaa ggtaaaattg ttcatcgtgc attgactcgc
     3721 cgccatcacg cgcagcgcgc cgaacaaggc gttcgtcaca caggtcgagg tttcgacgtt
     3781 gccggcgacc accgcggccg gagcccgcgg attcaacatg cagccctcgg gcaggatgat
     3841 ctgtaatggc ttgagacatc ccgcattgag cggaatgtca tcatccacca gcgtgcggaa
     3901 cacatacagg acggcggcgg tggtgatcgc ccgtggcgcg ttaaaattgt tcggcaactg
     3961 agcgctggtg ccggtgaaat cgacgcaggc gctgcgtcgt agcgcgtcga cgcgaacact
     4021 taagcggatg ttggccccgt tgtcgagcgg caacaagaac tcgccgtcct tgagcgccgt
     4081 gatcacgcgg cgcaccgatt cctcggcgtt gtcctgtacg tgccgcatgt aggcgaccac
     4141 ggtcgggcgg ccatactggg cgaccgtcga cttgagttcc tgcgcacctt tctcattcgc
     4201 cgcgatttgc gcgcgcagat cggcaagatt ctgcgtgatg ttgcgtgacg gatgaggccc
     4261 tgccgccagt aattcccgca gtgcagcctc gcgccacgca ccgcgctcga ccagcttgaa
     4321 attgtcgatc agcactccct cctcggcgag gctggtcgag aacggcggca ttgagcccgg
     4381 ggtggttccg ccgatgtcgg cgtgatggcc gcgcgaggcc acgaagaatc ccggcacgag
     4441 gtcggcggca tcgagataga ccggagtcac caccgtcacg tccggcagat gggtgccgcc
     4501 atggtaggga tcgttcaaga catagacgtc gccgggctgc atcgcggggt tttgcgcgat
     4561 caccgtgcgg atcgactcgc tcatcgagcc taaatgcacg ggcatgtgcg gcgcgttggc
     4621 gatcaacgaa ccctcggcgt cgaacagggc gcaactgaaa tccaatcgtt ccttgatgtt
     4681 caccgaaaac gccgtgttct gcaggcgcaa gcccatttgc tcggcaatgt tcatgaacag
     4741 gttgttgaag agctcgagcc tcaccgggtc gacgcgggta ccgggcgcgt ggcgcgcagc
     4801 gcgcgcgcgg acacgctgca tgtcgaggca gccagcggcg ctcaccgtca cccgccagcc
     4861 cggttcgaca atcgtggtcg cattgcgctc ggcgagcact gcaggaccgt cgatcaccgc
     4921 accgctgcgc aacgcctcga cccgataaag tccggcgtca cgccggcccg cgggcgcatc
     4981 gtcggccaga caatacatgc tgaccgtcgc atccgcgctt gcagcgaact cgctcgccgc
     5041 atcggcggtg gccgcgcgcg acgccgcggc gctcggtacg ccga
//
LOCUS       DASWZT010000226         4298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_21082, whole genome shotgun sequence.
ACCESSION   DASWZT010000226 DASWZT010000000
VERSION     DASWZT010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21082"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VGE92_06230"
     CDS             complement(<1..538)
                     /locus_tag="VGE92_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313451.1"
                     /translation="MSTHYFRFDCGAAAPQRAPLLERLLARADPATAAVNWRVDAFRV
                     LAPHTAAPGVAAAALFAEWGPANSAETVFIATPVHYVAEMSDVRLAAQGILTLRQAES
                     AALAEDFNHVWRGSGIRLHAGCRAVLFCLVAGALDAVTRDPEDVLDRHIADHLPSGAG
                     AARLRRLMSEIEMWLFEHG"
     gene            571..954
                     /locus_tag="VGE92_06235"
     CDS             571..954
                     /locus_tag="VGE92_06235"
                     /inference="COORDINATES: protein motif:HMM:NF016326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HEY1313452.1"
                     /translation="MRFTQDTSSQTNVIRAYSPGELRINDDTYRETVMVSGSTIVIVP
                     DVRVVDDLPRFDVSQLLALEPELVLLGTGPAQVFPAAAFRAKFLRAGIGFEAMDTGAA
                     CRTFNVLVAEQRRVAALLMVRKSAE"
     gene            complement(1192..1794)
                     /locus_tag="VGE92_06240"
     CDS             complement(1192..1794)
                     /locus_tag="VGE92_06240"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY1313453.1"
                     /translation="MGLCLVLGACADTARDGAQTYVVAPGDSLYSIAWRHNLDYRDLA
                     RRNNIGADFRISIGQVLLLARSVPQREVQPVPSAPTAATWVWPTARRGAPRPVPGGGI
                     LLPGRLGQDVRAASAGRVVYTGNGLRGYGNLIIVKHADNLLSAYAHNRELQVHEGQEL
                     AAGQVIAHMGVDAQQAPNLYFEIRQNGRPVNPLAFLSREK"
     gene            complement(1824..2489)
                     /locus_tag="VGE92_06245"
     CDS             complement(1824..2489)
                     /locus_tag="VGE92_06245"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019752402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEY1313454.1"
                     /translation="MEGPRLSGIGMTSARTRERLVQRLQEQGIADLRVLDRIRNVPRH
                     LFMDEALASRAYEDTALPIGFGQTISQPYVVACMTEVLLAAGGMANVLEIGTGCGYQT
                     AVLAPLVEKIYTIERIESLLGRARRTLRELRIRNVSFRHDDGNIGWVARAPFDGILLT
                     AAPHAVPPPLFEQLRVGGRLIAPVGPEGRQELYCYTKGETRTERRSLGPVSFVPLLSG
                     LQR"
     gene            complement(2494..3258)
                     /gene="surE"
                     /locus_tag="VGE92_06250"
     CDS             complement(2494..3258)
                     /gene="surE"
                     /locus_tag="VGE92_06250"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEY1313455.1"
                     /translation="MKILLSNDDGYRAEGLIALGAALRPLGAVTVVAPDRNRSGASNS
                     LTLDVPVRAARYDADAYYVNGTPTDCVHLAISGLFDFEHDIVVSGVNDGANLGDDTLY
                     SGTVAAAVEGRFLGLPAIAVSLCVEPGSPRNFSAAAQVAAQLVARMAQDRLQGPETAS
                     MILNVNVPDLPEGQLRGIRVTRLGSRHRSKPIVKAKDPRGRNVYWVGNAGSGQDAGPG
                     TDFHAVAAGFASITPLQIDLTRHAALPELQKWLKLE"
     gene            3353..3913
                     /locus_tag="VGE92_06255"
     CDS             3353..3913
                     /locus_tag="VGE92_06255"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEY1313456.1"
                     /translation="MTKQRPTGRADQAAEDAQAFRAAVRDVTPLAAAPAVAGLAKPKP
                     RLRVGHAATSALQDLDDSMPLASSADAVTGEQGLSFQRAGVRGQVLRKLRRGLFPVDD
                     ELDLHGLTQTAARSELVQFISRSRDRGCRCVRVIHGKGYRSGARGPVLKTAVNLWLRR
                     QLDVMAFVSARAIDGGSGAVYVLLRQ"
     gene            complement(3937..>4298)
                     /gene="truD"
                     /locus_tag="VGE92_06260"
     CDS             complement(3937..>4298)
                     /gene="truD"
                     /locus_tag="VGE92_06260"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HEY1313457.1"
                     /translation="GSHFTVSAVDDELRRRLKVFDIHPSGPLWGRGDPASSGRALQHE
                     MAVAQELAPIIGLLQREGLSQERRALRCAIRELAVERDGNTVCLSFLLGRGEFATAVL
                     REICDLDAVPGLDSDQE"
BASE COUNT          812 a         1408 c         1337 g          741 t
ORIGIN      
        1 cgccatgctc gaacagccac atctcgattt cgctcatgag ccgccgcagg cgcgcggcgc
       61 cggcaccgct cggtagatgg tccgcaatgt ggcgatcaag tacatcctcc ggatctcgcg
      121 ttaccgcatc aagtgcgccg gcgacgaggc aaaacaaaac ggcacggcac ccggcgtgca
      181 ggcgtattcc tgagccccgc cacacgtggt tgaagtcctc cgccagcgca gcgctctccg
      241 cctggcgcag ggtgaggata ccttgggcag ccaagcgcac gtcgctcatc tcggcgacgt
      301 aatgcaccgg cgtcgcgatg aataccgtct cggcagaatt cgccgggccc cactcagcga
      361 aaagcgcggc ggcagccacg cccggcgcgg ccgtatgcgg cgccagcaca cggaacgcgt
      421 cgacacgcca attgacggcc gcggtggccg gatccgcgcg cgccagcagg cgctccagca
      481 acggcgcgcg ttgcggggcc gcggcgccgc agtcaaatcg gaagtaatgg gtagacacga
      541 acgctccggc gcgaagagta tcttacgcat atgcgattta cccaagacac gagttcccag
      601 accaacgtga tccgcgcgta cagcccgggc gaactgcgca tcaacgacga cacataccgc
      661 gaaaccgtca tggtcagcgg ctcgaccatc gtcattgtgc cggacgtccg ggtggtggac
      721 gatctgcccc ggttcgatgt gtcgcagctg cttgcactcg agcccgagct cgtcctcctg
      781 ggaacggggc cggctcaggt tttcccggcc gcggcctttc gcgcaaagtt cctgcgcgcg
      841 ggcatcggtt tcgaagcgat ggatacaggc gcagcctgcc gcacattcaa cgtactggtg
      901 gccgagcagc gccgggtagc ggcgttgctc atggtgcgaa aatcagcgga gtaaaatggc
      961 agggcgctta cggaatccac gatcaatacg gtatcgagat atattgttct tcttcttgat
     1021 cttgttactc ttttgtccct ctgaacccgt gtaaacctca gcgccccgca aatggacttt
     1081 cccgatcgag ggctagttaa ccacacgaaa tgcacagtcg caagatggcg acagatggct
     1141 gtaaagagct aaatttagat atgtcaaagc cttacgcgac aaacgtagaa atcacttttc
     1201 tctagaaagg aacgccaacg ggttgaccgg tctgccattt tggcgaattt cgaaatacag
     1261 gttcggtgct tgctgcgcat cgactcccat gtgcgcgatc acttggccgg cggccagttc
     1321 ctgaccctcg tgaacttgca actcgcgatt gtgcgcgtac gcggaaagca agttgtccgc
     1381 gtgtttgacg atgatcagat ttccataacc gcgcaaaccg ttgccggtat agaccacgcg
     1441 gccggcgctg gccgcgcgca cgtcctgccc caagcgcccc ggcagcagaa taccgccgcc
     1501 gggcacgggt cgcggcgcgc cgcggcgcgc cgtcggccat acccaggttg ctgcggtggg
     1561 cgcgctcggc accggctgga cctcgcgctg cggcacgctc cgagccagga gcaacacctg
     1621 cccgatcgag atgcggaaat ccgcgccgat gttgttcctt cgcgccaggt cgcggtagtc
     1681 gaggttgtgg cgccacgcga tgctgtagag cgagtccccg ggcgcgacca cgtaagtctg
     1741 cgcaccgtcg cgtgcggtat cggcacatgc gccgagcaca aggcacagcc ccacggcgca
     1801 aatcctgggc gggccacgca actttagcgc tgcaatcccg atagcaaggg gacgaaactg
     1861 acgggaccga gcgagcgacg ctcggtcctg gtctcaccct tggtgtagca ataaagttcc
     1921 tggcggccct ccggccccac cggcgcgatg agacggccgc ccacgcgaag ttgttcgaac
     1981 agcggcggcg gcacggcatg cggcgcggcc gtcagcaata ttccatcgaa cggcgcgcgt
     2041 gccacccatc cgatgttgcc atcgtcatga cggaaactga cattgcgaat ccgcagttcc
     2101 cgcagggtgc gtcgtgcgcg tcccagaagg ctctcgatcc gctcgatggt gtatatcttt
     2161 tccaccaacg gcgccagcac cgcggtctgg tagccacagc cggtgccgat ctccaagaca
     2221 ttagccatcc cgcccgccgc cagcaagacc tcggtcatac aggccaccac atagggctga
     2281 gagatggttt gtccgaatcc gatcggcagc gccgtgtcct cgtacgcgcg gctggccaat
     2341 gcctcgtcca tgaacagatg acgcggcacg ttgcgaatcc tatcgagcac gcgcagatcc
     2401 gcgattccct gttcctgcaa acgctgcacc aggcgttcgc gggtgcgtgc cgaggtcatg
     2461 cctatgccgc tgagtcgcgg tccttccata aagctactca agcttgagcc acttttgcag
     2521 ttcaggaagc gcggcgtggc gcgtcaagtc gatctgtagc ggcgtaatcg aggcgaaacc
     2581 cgcggcgacc gcatgaaaat ccgtgccggg gccggcgtcc tgaccgctgc ccgcgttgcc
     2641 cacccaatac acattgcggc cgcgcggatc cttcgccttg acgatgggtt tgctgcgatg
     2701 gcggctgccg agacgagtca ctctgatgcc gcgcaattgc ccctcgggca aatcgggtac
     2761 gttgacgttg aggatcatcg acgcggtttc cggcccctgc aaacgatctt gtgccatgcg
     2821 ggcgacgagc tgcgccgcca cctgggcggc cgccgagaaa tttcgcgggc ttccaggctc
     2881 gacgcacaat gaaaccgcga tcgccggaag tccgagaaag cggccctcga cggcggcggc
     2941 gacagtgccc gaatacaatg tgtcgtcgcc gagattggca ccgtcattga cccctgatac
     3001 gacgatgtcg tgctcgaaat cgaacagacc ggaaatcgcc agatgcacgc agtcggtcgg
     3061 ggtgccgttc acgtagtagg cgtcggcatc gtagcgtgcg gcgcgcaccg gcacatcgag
     3121 cgtgagggag ttgctggcgc cgctgcggtt cctgtccgga gccaccaccg tcacggcacc
     3181 caagggccgc agcgccgccc caagcgcaat taaaccctcg gcacggtagc cgtcgtcgtt
     3241 tgaaaggaga atcttcacga aaattaacag cctgccgcaa gcgtggcggg ggtcgccgtg
     3301 cggactatac tgtaacgctg tagaccccga taggcgatcg agttgtccat ccatgaccaa
     3361 acaacgcccc acgggccgcg ctgaccaagc ggcggaggat gctcaagcat ttcgcgcggc
     3421 ggtgcgcgat gtcacgccgt tggccgcggc gcccgcggta gccggactcg cgaagcccaa
     3481 accacggctg cgcgtcgggc acgccgcgac atcggcgctg caagatctcg atgacagcat
     3541 gccgcttgcg tcgagcgcgg atgccgtgac gggggagcaa ggcttgtctt ttcagcgcgc
     3601 aggcgtgcgc ggccaggttt tgcgcaagct gcggcgcggg cttttcccgg tagacgatga
     3661 attggatctt catggactga cgcaaacagc cgcccgcagc gagcttgtgc agttcatctc
     3721 acgcagccgc gaccgcggtt gccgctgcgt gagagtcatc cacggcaagg gctatcggtc
     3781 cggtgcgcgc ggcccggtgc tcaaaaccgc cgtgaatctg tggttgcgcc ggcaattgga
     3841 tgtgatggca ttcgtctcgg cgcgcgccat cgacggcggc agcggcgcgg tgtacgtgct
     3901 gctgcggcag tgaccttgga aacccgcagc ggcagcctat tcctggtcgg agtcgagtcc
     3961 cggcaccgca tcgaggtcgc agatctcgcg caatactgcg gtcgcaaact cgccccggcc
     4021 gagcaagaag ctcaaacaca cggtgttgcc gtcccgctcg accgccagtt cgcgaatcgc
     4081 acaacgcagc gcgcgccgct cttggctcaa tccctcgcgc tgcagtaaac cgattatcgg
     4141 cgccaattcc tgcgccaccg ccatttcgtg ctgcagggct cgtccgctgc tcgcgggatc
     4201 gccccgcccc cacaacgggc ccgagggatg aatatcgaac accttcagcc gccggcgcaa
     4261 ctcgtcgtcg accgcgctca cggtgaagtg gctgccgc
//
LOCUS       DASWZT010000227         1568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_64647, whole genome shotgun sequence.
ACCESSION   DASWZT010000227 DASWZT010000000
VERSION     DASWZT010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64647"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VGE92_06265"
     CDS             complement(<1..214)
                     /locus_tag="VGE92_06265"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEY1313458.1"
                     /translation="MEALARMSVLRWHPMVVHFPLALVTTATLCLLSARLLRHKGPTA
                     TLATVGTWNLCLGALAAVLALATGLAA"
     gene            complement(193..969)
                     /gene="rfbF"
                     /locus_tag="VGE92_06270"
     CDS             complement(193..969)
                     /gene="rfbF"
                     /locus_tag="VGE92_06270"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HEY1313459.1"
                     /translation="MKCVILAGGKGTRIAEESSTRPKPMLEIGSRPILWHIMKLYASF
                     GVTEFIVCLGYKGYVIKEYFANYFLHQADVTFDMAENRVDYHDCRSEPWKVTLVDTGE
                     ETMTGGRLLQVGKYLAADEPFCLTYGDGLANIDIRAELAFHADHGRKATVACVRPPAR
                     FGRIVSQGGRVVSFEEKPTAEGGLINGGFFVLEPSVLELIDGNNTAWEKGPMENLASV
                     GQLAAWVHRGFWQPMDTLRDKLTLNALWESGSAPWKLWQG"
     gene            complement(966..>1568)
                     /locus_tag="VGE92_06275"
     CDS             complement(966..>1568)
                     /locus_tag="VGE92_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HEY1313460.1"
                     /translation="PYSNSKACTELVTASFRDSFFADGAPIATARAGNVIGGGDWSQE
                     RLIPDCVRALESGEPVSLRYPDAVRPWQHVLEPLSGYLSLAQGLVTAPEKTPRAVNFG
                     PDPASFCTVRAVVETFAARFAGYPRWVRDPAPQPPEAEALTLASTLAAQSLGWRPRLD
                     IGASVSWAADWYWAHSAGENMLAFSKAQIARYRELMKRSP"
BASE COUNT          304 a          532 c          463 g          269 t
ORIGIN      
        1 ccgccgccag tccggtcgcg agcgccaaca ccgcagcgag cgcgccgaga catagattcc
       61 aagtgccgac cgtggcgaga gtcgccgttg gacccttgtg ccgcaacaag cgcgcgctca
      121 atagacacaa cgtggcggtg gtgacgagcg ccagcgggaa atgcaccacc atcggatgcc
      181 agcgcagaac gctcatcctt gccagagctt ccacggcgcg ctaccgctct cccagagcgc
      241 gttcagggtc aatttgtccc gcaacgtatc catcggctgc caaaaaccac ggtgcaccca
      301 cgcggccaat tgaccaaccg acgccaagtt ctccatcggc cccttctccc aggcggtgtt
      361 gttgccgtcg atcaattcga gcacgctcgg ctccaagacg aagaaaccgc cgttgatcaa
      421 accgccctcc gccgtcggct tctcctcgaa cgagaccact cggccgcctt gcgagacgat
      481 tcggccgaac cgcgccggcg gacgcacgca agccacggtc gccttcctgc cgtgatcggc
      541 gtggaatgcg agctcggcgc gaatatcgat gttcgcgaga ccgtctccat aggtcaggca
      601 aaacggctcg tccgcggcaa gatacttgcc cacttgcaag agtcgtccgc cggtcatggt
      661 ctcctcgccg gtatcgacca gcgtcacctt ccacggttcg ctgcggcagt cgtgatagtc
      721 gacgcggttc tccgccatgt cgaacgtcac atcggcctga tgcaggaaat agttcgcgaa
      781 gtattccttg atgacgtagc ccttgtagcc caggcagacg atgaactccg tgacgccgaa
      841 tgacgcgtac agtttcatga tgtgccacaa aatgggccgc gaaccgattt ccagcatggg
      901 ctttggccgg gtactcgact cctcggcaat gcgcgtgccc ttgccgccgg cgagaatcac
      961 gcacttcatg gcgagcgctt catcagttcg cgatatcgtg caatttgcgc cttggaaaat
     1021 gccagcatgt tctcccccgc tgaatgggcc caataccaat ccgccgccca cgacactgac
     1081 gcgccgatgt cgagccgcgg gcgccagccg agcgattgcg ccgccagcgt cgatgcaaga
     1141 gtcagagcct cggcctccgg cggctgcggc gccggatcgc gaacccagcg cggatagcct
     1201 gcgaaacgcg ccgcaaacgt ctcgaccact gcacgtaccg tgcagaagga tgccgggtcc
     1261 gggccgaaat tgacagcacg cggcgttttt tccggcgccg tcaccaagcc ttgcgccaag
     1321 ctcagatagc cggagagagg ttcgagcaca tgctgccaag gccgcacggc atcggggtag
     1381 cgcaggctca cgggctcccc ggattcgagc gctctgacgc agtcgggaat gagtcgctcc
     1441 tgcgaccagt ctccgccgcc gatcacgttg ccggcgcgcg ccgtcgcaat gggcgcgccg
     1501 tcggcgaaga agctgtcgcg aaaactcgcg gtcaccagtt ccgtacaggc tttgctattg
     1561 ctgtaggg
//
LOCUS       DASWZT010000228         4502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_21090, whole genome shotgun sequence.
ACCESSION   DASWZT010000228 DASWZT010000000
VERSION     DASWZT010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21090"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VGE92_06280"
     CDS             <1..702
                     /locus_tag="VGE92_06280"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1313461.1"
                     /translation="FLETDLSPGIELGTATKKIYALPTLKHLPQGVHLVETGDAEFMN
                     ELYQNFLLAVGAGVLMVFAVLVLLFVRVLQPITILSALPLSLGGAVLALLLTGLPFSL
                     PVVIGILMLMGIVGKNSILLVDFAIEEMRAGKPRLEAILEAGHKRARPIVMTTVAMVA
                     GMLPVAIGWGGDSDFRGPMAIAVIGGLITSTSLTLVIVPAVFTLFDDIERWIAPKAGK
                     LLAERPQPAAPEPAV"
     gene            699..1541
                     /locus_tag="VGE92_06285"
     CDS             699..1541
                     /locus_tag="VGE92_06285"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY1313462.1"
                     /translation="MKLPLLAASLACTVALTSPPQAAHADVLAAVNSARQRGCHAAAA
                     PRALASNPQLQKAAARVAGGAPLRKALASVGYLAAESFELHFSGAVDDVRITQALTAR
                     YCQTLRDPALRELGADRHGSELWMVLAAPVALPAPADAAAVNRRILDLVNGARAAGGR
                     CGATYFAPVAALELNAKLTSAALAHSRDMAQSGQFDHRGHDGSTPASRVESAGYGAHR
                     AVGENIAAGAMTPAEVTAGWLASPPHCENIMDGRFTQIGIGYAANLNSDVGMYWTQDF
                     AAPR"
     gene            complement(1538..1969)
                     /locus_tag="VGE92_06290"
     CDS             complement(1538..1969)
                     /locus_tag="VGE92_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY1313463.1"
                     /translation="MPDLEERFSAALHNTSRAWRHALDRRIKYLGVSQASWMTIAVAA
                     KASEPLSQSELADRLGVEGATMVAMVDRLVKAGFALREPSTRDRRIKRVVLTKAGNLL
                     FDKVRAEATAFREELLVGTDAKQLLLATELLEDLQRLIEAR"
     gene            2112..2738
                     /locus_tag="VGE92_06295"
     CDS             2112..2738
                     /locus_tag="VGE92_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY1313464.1"
                     /translation="MKSASLIPTAKFSEAVLPRRSVADGQFLDPDDPAVLAITDFTRE
                     YPVTVDEDRQIDDALGDMIHLGVRAMLVMREQRIVGLITSYDIQGERPLQFLQTSTYR
                     RHQEIRVGHVMTPWDKLLALDWDGILASRAGDLLHMLEEEGLTHLLVIEPGTKHAAPV
                     VRALVSRARLARQLARTAARALALQPDDGGNPGKRHHDRADQVSRHSA"
     gene            complement(2655..3035)
                     /gene="crcB"
                     /locus_tag="VGE92_06300"
     CDS             complement(2655..3035)
                     /gene="crcB"
                     /locus_tag="VGE92_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEY1313465.1"
                     /translation="MWRAFVAVAIGGMLGCLLRWALAMFLNRYFPSIPPGTLAANLIG
                     CYIIGMALAFFTTYPHFAPEWRLFVTTGFCGGLTTFSTFSAEVVLLLQSGRVAWALGA
                     VATHLIGSIMMTFAGIATIVWLKS"
     gene            3136..4470
                     /locus_tag="VGE92_06305"
     CDS             3136..4470
                     /locus_tag="VGE92_06305"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HEY1313466.1"
                     /translation="MKKPNIHTDLPGPKARELLARDALVVSPSYPRDYPFVMSHGKGA
                     EIWDVDGNRFLDFAAGIAVCSTGHSHPAVVQAIKDAADRFLHISSDYWHEGQIKLAER
                     LNELSPMKEPVMSFFAQSGTESVEAALKLARFTSGRGRFIGFLGGFHGRTMGSLSFTS
                     SKYTQQRGFFPSMPGVTHVPYPNPYRPLFAGADQGRAVLRYIEEVLFVHNVPASEVAA
                     ILIEPLQGEGGYLVPPDGFLQGLRELCDRHGILLIFDEVQSGIGRTGKMFAAEHWDVA
                     PDIMTLAKGLGSGLPIGMVVAKRSVMQKWSRGAHGNTFGGNPLCCAAALATLDLVERE
                     YCANAAKVGAYFLRKLHALAEKFAVVGDVRGKGLMIGVELVKDRTSKEPAKKLCDALI
                     TRAYHNGLILLACGQSTVRFMPPLVIDAADVDEAIVLLERSLSEVLASERVA"
BASE COUNT          847 a         1382 c         1438 g          835 t
ORIGIN      
        1 ttcctcgaga ccgatttgag cccggggatc gaactgggta ctgccaccaa gaaaatctac
       61 gcgctgccga cgctcaagca tctgccgcag ggggtccatc tggtcgagac cggcgacgcc
      121 gaattcatga acgagctgta ccagaacttc ttgctggccg tcggcgccgg cgtgctgatg
      181 gtgtttgcgg tgctggtgct gctgttcgtg cgcgtgctgc aaccgatcac gattctgtcg
      241 gcgctgccgc tgtccctcgg gggtgcggtg ctcgcgctat tgctcaccgg gctgccattc
      301 tcattgcccg tggtgatcgg tatcttgatg ttgatgggaa tcgtcggcaa gaactcgatc
      361 ctgctggttg acttcgcgat cgaagaaatg cgcgcgggta aaccgcggct cgaagcgata
      421 ctcgaagccg gacacaagcg cgcgcggccc atcgtcatga ccacggtggc catggtggcg
      481 ggtatgctgc cggtcgccat cggttgggga ggcgacagcg actttcgcgg tccgatggcg
      541 atcgccgtga tcggcggact catcacctcc acgtcgctca ccttggtcat cgtacccgcg
      601 gtgtttacgc tgttcgatga tatcgagcgc tggattgcgc cgaaggccgg gaaactgctg
      661 gccgagcggc cgcagccggc cgcgcctgaa cccgccgttt gaaacttccg ttgctggccg
      721 cgagcctggc ttgcacggtt gcgctgacct cgccgccgca agccgcgcat gccgatgtgc
      781 tcgccgcggt aaattccgcc cggcagcgcg gctgccatgc cgccgcggcg ccgcgcgcgc
      841 tcgcgagcaa tccgcaactg cagaaagcgg cggcacgagt ggccggcggt gcgccgctgc
      901 gcaaggccct ggcctcggtc gggtatctcg ccgccgaatc tttcgagctg catttttccg
      961 gtgcggtcga cgatgttcga atcacccagg cgctgacggc ccgctactgc cagaccttgc
     1021 gcgatccggc gctgcgcgag cttggcgcgg accgtcatgg cagcgagctc tggatggtgc
     1081 tcgcagcccc ggtggcgttg ccggcgcccg ccgatgccgc cgccgtcaat cggcggatac
     1141 ttgatctcgt gaatggcgca cgcgccgccg ggggccgctg cggtgcgact tactttgcgc
     1201 cggtcgccgc gctcgagctc aacgcaaaat tgacgagcgc cgccctcgcg cactcacgcg
     1261 acatggcgca gagcggccaa ttcgatcacc gcggacacga cggcagtacg cccgcgagcc
     1321 gcgtcgagag cgctggctac ggggcacacc gagcggtggg agagaatatc gcggcgggtg
     1381 cgatgacgcc ggccgaagtc accgccgggt ggctcgcgag tccgccgcat tgcgagaaca
     1441 tcatggacgg gcgcttcacg cagatcggaa tcggttacgc ggcgaatctg aacagcgatg
     1501 tcggcatgta ctggacgcag gatttcgcgg cgccgcgcta acgcgcctcg atcagacgct
     1561 gcaagtcctc cagaagttcg gtcgccagca gcagctgctt agcgtcggta ccgacgagga
     1621 gctcctcgcg aaatgcagtg gcctccgcgc gcaccttgtc gaacagcaga ttgcctgctt
     1681 tggtgagcac gacccgcttg attcgccggt cgcgcgtcga tggttcgcgt agcgcaaagc
     1741 ccgcctttac gagccgatcg accatcgcca ccatggtcgc gccttccact cccagacgat
     1801 cggcgagttc ggattgagac agcggctcgg aggctttggc ggccaccgcg atggtcatcc
     1861 aactcgcctg gctgacgccc aagtatttga tccgcctgtc caaagcgtgc cgccaggcgc
     1921 gcgacgtatt gtgcaacgct gcgctgaagc gctcttcgag atccggcatt tacaaaggac
     1981 tctaatggtt agtgccctaa ttaacatagc acgccttgcg gcaaacaaaa aggggggcgt
     2041 gcgtagacac ggcgcggcgt ttgcgtaagc atgcaatgag ttcaccgctg gccacccgag
     2101 ggttagtgtt catgaaaagc gcatcgttga taccgaccgc aaaattcagc gaagcggtgt
     2161 tgccgcggcg ctcggtggcc gacggtcaat ttctcgatcc ggacgaccct gcggttctgg
     2221 cgattaccga cttcacccgc gaatatccgg tcacggtcga cgaagatcgg cagatcgacg
     2281 atgccttggg cgacatgatt cacttgggag ttcgcgccat gctggtgatg cgcgaacagc
     2341 gcatcgtcgg attgatcacc tcgtatgata ttcaaggcga gcggccgctg cagttcctgc
     2401 agacatcgac ctaccggcgc caccaggaaa ttcgcgtcgg ccatgtgatg acgccgtggg
     2461 ataaattatt ggcgctcgac tgggatggca tcctcgcgtc gcgcgccggc gatctgctgc
     2521 acatgctgga ggaggagggg ctcactcatt tgctggtgat cgagcccggc accaagcatg
     2581 cggcgccggt ggtgcgcgcc ctggtttcgc gcgcgcggct ggccaggcag ctggcgagga
     2641 ctgcggcgcg agcgctagct cttcaaccag acgatggtgg caatcccggc aaacgtcatc
     2701 atgatcgagc cgatcaagtg agtcgccaca gcgcctaacg cccaagccac gcggccggat
     2761 tgcagcagca gcacgacctc cgctgagaac gtcgagaatg tggtcaagcc gccgcagaaa
     2821 cccgtcgtga cgaacaagcg ccattccggc gcgaagtgcg gataggtggt gaagaacgcg
     2881 agcgccatgc cgatgatgta gcagccgatg agattggccg ccaaagttcc gggtgggatg
     2941 ctcggaaaat agcggtttaa aaacatggcg agagcccagc gcaacaggca accgagcatg
     3001 ccgccaatcg ccaccgccac gaatgctcgc cacattatcg gcagcgatct tgcaagcaac
     3061 ggtcattgac agatcgcatc gcacagatcg taatcgcttt gccgaagcga atcaaaaaaa
     3121 tccgggagtc agttcatgaa aaaaccgaat atacataccg atttgccggg tcccaaggct
     3181 cgagagttgc tggcccgcga tgcgttggtg gtctcgccgt cctatccgcg cgactacccc
     3241 ttcgtcatgt cccacggcaa gggtgcggag atctgggacg tcgacggcaa tcgatttttg
     3301 gacttcgccg ccggaattgc cgtgtgcagt acgggtcata gccatcccgc ggtggtgcag
     3361 gccatcaagg atgcagccga tcgtttcctg cacatctcct ccgactactg gcacgaaggg
     3421 cagatcaagc tggccgaacg cctcaacgaa ttgtcgccca tgaaagaacc cgtcatgagc
     3481 ttctttgcgc agtcgggcac cgaaagcgtg gaggcggcgt tgaaactggc gcgcttcacc
     3541 agcggacgag gccgcttcat aggatttctc ggcggctttc atggtcgaac catgggctcg
     3601 ctgtccttca cctccagcaa gtacacgcag cagcgaggtt tttttccgag catgccgggc
     3661 gtgacgcatg tgccgtatcc gaatccgtac cggccgctgt tcgccggtgc ggatcaaggt
     3721 cgcgcggtgc tgcgttacat cgaggaggta ttgttcgtgc acaacgtgcc ggcgagcgaa
     3781 gtcgcggcga tactcatcga gccgctgcag ggcgagggcg gctacctggt gccgccggac
     3841 ggttttctgc agggcttgcg cgaattgtgc gaccggcatg gcatcttgct gatattcgat
     3901 gaggttcaat ccggcatcgg ccgcaccggc aagatgttcg ccgcagaaca ttgggatgtc
     3961 gcgccggaca tcatgacact ggcaaaagga ttgggatccg gacttcccat cggaatggtc
     4021 gtcgcgaagc gcagcgtcat gcagaagtgg agccgcggcg cgcacggcaa cacgtttggc
     4081 ggaaatcctc tgtgttgcgc cgctgcgctc gccaccctcg atttggtcga gcgcgagtat
     4141 tgcgccaacg ccgccaaagt cggcgcctac ttcctgcgca agctgcacgc actcgccgaa
     4201 aagttcgcgg tcgtcggcga tgtgcgcggc aaaggcttga tgatcggcgt cgaattggtg
     4261 aaggatcgaa ccagcaagga gccggccaag aagctctgcg acgcgctcat cacacgcgcc
     4321 tatcacaacg gtctgatctt gctggcatgc ggacagagta cggtgcgctt catgccgccc
     4381 ctggtgatcg acgcagcgga tgtcgacgaa gcgatcgtgc tactcgagcg cagtctgagc
     4441 gaagtgctcg ccagcgaacg cgtcgcttga atcgacctgc cgcagttgcc gcaacagaga
     4501 ct
//
LOCUS       DASWZT010000229         1840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_40574, whole genome shotgun sequence.
ACCESSION   DASWZT010000229 DASWZT010000000
VERSION     DASWZT010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40574"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VGE92_06310"
     CDS             <1..184
                     /locus_tag="VGE92_06310"
                     /inference="COORDINATES: protein motif:HMM:NF019940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HEY1313467.1"
                     /translation="AQKMLTDGGPVGRRLDFLAQELNRESNTLTAKANDVELTNIGLE
                     LKSTVEQFREQVQNLE"
     gene            187..840
                     /gene="gmk"
                     /locus_tag="VGE92_06315"
     CDS             187..840
                     /gene="gmk"
                     /locus_tag="VGE92_06315"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEY1313468.1"
                     /translation="MAKALPETVAIARRGLMLVLSSPSGAGKTTLSRRLLEQDYGVAL
                     SVSVTTRKMRPGEVDGHHYTFIDRRQFDALIAKNELLEWAEVFDNYYGTPAQPVRDAL
                     AEGRDVLFDIDWQGTQQLSEKARGDLVSVFILPPSIPELERRLKARAQDDYETIHRRM
                     AKAADEMSHWAEYDYVVINRDLDQAFTDVRAILAAERLKRERQPGLSDFVRGLQAKL"
     gene            893..1093
                     /locus_tag="VGE92_06320"
     CDS             893..1093
                     /locus_tag="VGE92_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313469.1"
                     /translation="MSNGSTLSRSELDDRIAILRDNIRQLIEQAAAYSGAEDEDRSAD
                     RIAQQTQELETLIKQRDALPKK"
     gene            complement(1104..1835)
                     /gene="rsmA"
                     /locus_tag="VGE92_06325"
     CDS             complement(1104..1835)
                     /gene="rsmA"
                     /locus_tag="VGE92_06325"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEY1313470.1"
                     /translation="MRIARTGGPLEGATVIEVGPGPGGLTRALLTLGAKRVIAIERDQ
                     RAIAALEEIAAHYPGRLEIVADDALRFDARAHLGGNRAHVIANLPYNIATALLIGWLT
                     AEPWPPWYDALVLMFQREVGERIAAAPGSKSYGRLSVLANWRCETKILFDVAPSAFVP
                     APKVTSSVVRLVPRAAPLACDALALQRLTEAAFGQRRKMLRQSLKTLGVDAGALLAAA
                     GIEPTARAEEIPVEGFVKLANALTA"
BASE COUNT          366 a          549 c          610 g          315 t
ORIGIN      
        1 ggcgcagaag atgctcaccg acggcgggcc ggttggccgc aggctcgatt ttctggcaca
       61 ggaactcaac cgcgaatcga atacgctcac cgccaaggcc aacgacgtcg agctgaccaa
      121 tatcgggctg gaacttaaga gcacagtcga gcaattccgc gagcaggtgc agaacctgga
      181 gtgacgatgg cgaaggcact accggagaca gtggcgattg cccggcgcgg gctgatgttg
      241 gtgctgtcgt cgccgtccgg cgccggcaag accacgctgt cgcgccgcct gctagagcaa
      301 gactatggtg tcgcgttgtc ggtgtcggtg accacgcgga aaatgcgtcc gggcgaggtg
      361 gacgggcatc actacacgtt catcgatcgc aggcaattcg acgcgttgat cgccaagaac
      421 gagttgttgg aatgggccga ggtgttcgac aattattacg gcacgccggc ccagccggtg
      481 cgcgacgcgc tggccgaagg ccgcgatgtg ctgttcgata tcgactggca gggcacccag
      541 caattgagcg aaaaagcgcg cggcgatctg gttagcgtgt tcatcttgcc gccttcgatc
      601 ccggagctgg agcggcggct caaggcgcgc gcacaggacg attacgagac catccaccgc
      661 cgcatggcga aggccgccga tgagatgagt cattgggcgg aatacgacta cgtcgtgatc
      721 aaccgcgatc tcgaccaagc tttcaccgac gtcagggcga ttctggccgc cgagcggctc
      781 aagcgcgagc gtcagcccgg cctgtccgat tttgtgcgcg gattacaggc gaagctttag
      841 atattctcgt gctaggcctt gcggtataaa gagcttcatt tggaggaaca tgatgagcaa
      901 tggcagcacg ctttcgcgtt ctgaactcga cgaccgcatc gcaattcttc gtgacaacat
      961 ccggcagctg attgaacaag ctgctgcata ttcgggtgcc gaggatgaag atcgaagcgc
     1021 ggaccggatc gcgcaacaga ctcaagaact tgaaacgctg atcaagcagc gcgatgcgct
     1081 accgaagaaa tagcgaattc tgtttacgcg gtcagcgcat tagcgagctt aacgaagcct
     1141 tcgaccggaa tttcctcggc gcgcgcggtt ggctcgatgc ccgcggcggc gagcagcgcg
     1201 ccggcgtcga cacccaaagt cttgaggctt tgccgcagca tcttgcgacg ctgcccgaaa
     1261 gcagcttccg tcagccgctg caacgctagg gcatcgcaag ctagcggcgc ggcgcgcggg
     1321 accagccgca ccacggagga cgtcactttg ggtgccggca caaaggcgga cggcgcgacg
     1381 tcgaacagaa tcttggtctc acagcgccaa tttgccagca ccgacagccg gccatagctt
     1441 ttgctgccgg gcgcggctgc gatgcgttcg ccgacttcgc gctggaacat cagcaccagc
     1501 gcgtcgtacc agggcggcca gggttcggcg gtgagccagc cgatcagcag cgcggtggcg
     1561 atattgtagg gcaggttggc gatgacgtgc gcccggttgc cgcccaggtg agcgcgcgca
     1621 tcgaagcgca gcgcgtcatc ggcaacaatc tcgagtcggc ccggatagtg tgcggcaatt
     1681 tcttcgaggg ccgcgatcgc gcgctgatcg cgctcaatgg cgatcacgcg cttggcgccg
     1741 agcgtgagca aggcgcgtgt caggccgccg gggccggggc cgacctcgat gacggtcgcg
     1801 ccctcgagcg gtccgccggt acgcgcgatg cgcaccgtca
//
LOCUS       DASWZT010000230         7498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_74296, whole genome shotgun sequence.
ACCESSION   DASWZT010000230 DASWZT010000000
VERSION     DASWZT010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7498
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74296"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VGE92_06330"
     CDS             complement(<1..962)
                     /locus_tag="VGE92_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY1313471.1"
                     /translation="MELKQLRASIDHTWEHSIVERLQAYIRIPNQSPHFDPQWQEHGH
                     MQRAVELMAEWCRAQPVPGMRVEIRRLPGRTPLLLIDVPGEVPGCVLLYGHLDKQPEF
                     TGWSPGLSPWEPVMREGRLYGRGGADDGYAVFSSLTAIVALKQQRVPLARCVLLIEAS
                     EESGSIDLPAHLEALGDAIGDPSLVICLDAECGDYARVWCTTSLRGNLTGQLRVRVLT
                     EGVHSGMASGIAATPFRIIQELLQRIESPKTGEVLLEELRVEIPADRLSQIAATARAL
                     GANVAGKLPWAPHAHAISADPKELIANNTWRAALAVTGADGLPAV"
     gene            complement(986..1975)
                     /gene="iolG"
                     /locus_tag="VGE92_06335"
     CDS             complement(986..1975)
                     /gene="iolG"
                     /locus_tag="VGE92_06335"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR04380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HEY1313472.1"
                     /translation="MLNVAIVGAGRIGQIHARNAAAAAGLTLKFVVDPNRAAAEALAQ
                     QTGARVADLDAVLGDATVSGVIIASATHVHLDQALAVASAGKAIFCEKPLDLDLARAR
                     QAGAPLAEAALLVGFNRRFDPHFAVLKARIDAQAIGKLETLTIISHDPAPPPVSYIRT
                     SGGLFKDMAIHDFDMARWLLGEEPTEVFASASCLIDPAIAAAGDVDTARIVLRTESGR
                     LCVISNTRRSGYGYDQRIEAYGSLGTVRADNVLMSTVTTCTERGAAGDALQNFFLERY
                     AEAYRREMAHFEAILRDRVKPLAGYHDGIAALALAEAAALSAKLNQPVRVASV"
     gene            2071..3960
                     /gene="iolC"
                     /locus_tag="VGE92_06340"
     CDS             2071..3960
                     /gene="iolC"
                     /locus_tag="VGE92_06340"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: protein motif:HMM:TIGR04382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HEY1313473.1"
                     /translation="MIAIGRSSVDLYGEQVGGRLEDMGSFAKYVGGSPTNTAIAAARL
                     GLKAALLTRVGADHMGRFIREELEREGVDVRGVHADPARLTALVVLGIRNRETFPLIF
                     YRENCADMALCEQDLDDGLLAEARAVLINGTHLSTPGVFAASVGAARRVKANGGRVVF
                     DVDYRPVLWGLTPKDLGENRFVENSQVTERLQSVLSLCDLIVGTEEELCILGGSVELL
                     AAIRNVRARTAATLVCKRGGHGCVAFAQEIPDRLEHGLVVPGFKIEVLNVLGAGDAFM
                     GGFLRGWLRDATLEECCRYGNACGAIVVSRHGCAPAMPTWGELEVLLSRRAKHPTESS
                     IGYLDQVHWASTRRREYPELTVFAIDHRSQFEELAEPSGPHGERIGRFKTLGLQALDR
                     VARRDPRFGVLLDGRYGFDALSQAADMPYWIGRPIEVPKSRPLEFEGSADVATEIATW
                     PLNQVVKCLVIYHPDDAAELRERQERQLLRLFDACRKTRHELLLEIIAPAGTRADART
                     TARAIEEIYARGIYPDWWKLAPGSKAHWQAIEETIMREDPRCRGVVLLGLGASAADLS
                     AAFATAAPFAIVKGFAIGRSVFQDTARAWFANSLSDGDACQAMADQLSTLVSAWRGAR
                     QALAS"
     gene            3957..5792
                     /gene="iolD"
                     /locus_tag="VGE92_06345"
     CDS             3957..5792
                     /gene="iolD"
                     /locus_tag="VGE92_06345"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR04377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HEY1313474.1"
                     /translation="MKTIRCTAAQATVRYLANQYIAGASGDVPYFAGVWAILGHGNVA
                     GLGEALYAAQKQIPTYRAHNEQGMALAAIAFAKQLRRRRAMICTSSIGPGATNMVTAA
                     ALAHVNRLPVLFLPGDVFASRGPDPVLQQIESFADGTVSANDCFRPVSRYFDRIARAE
                     QIIDALPRAMNTLLDSASCGPVTLAFCQDTQAEAYPYPEAFFRRREWSIRRPPGDRRE
                     LVQLVTLMRSARTPLIIAGGGVHYSGAQDVLAAFASSTRIPVAETQAGKGALAWNHPF
                     NLGAIGVTGTSAANEAAARADLVIGIGTRLQDFTTGSRALFTAPNRRLVQINIAAYDA
                     GKHEASTMVGDAGAVLSELARRMGDWRSANNEELIRADVAHWNNVWTQATAAGNALPS
                     DAQVIGALWRNSPDNAVVVCAAGGLPGELHKLWRSRERDDYHAEYGYSCMGYEIAGGL
                     GVKMAQPQREVYVMVGDGSYLMLNSELATSVTLGFKLVVIVLNNRGFGCISRLQRSTG
                     AAAFNNLWEPDARGAEIDFVAHAASLGAIAEKADSIQGLELALQRARESTRSHVIVID
                     TDPIKTTAEGGAWWDVAIPEVSSRHEVDAARTASLRERKNRRLGD"
     gene            5797..6702
                     /gene="iolE"
                     /locus_tag="VGE92_06350"
     CDS             5797..6702
                     /gene="iolE"
                     /locus_tag="VGE92_06350"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR04379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HEY1313475.1"
                     /translation="MSIRFGVSPIAWINDDLPALGGDTPLDSVLADAHEVGFAGIELG
                     GKFPKEPRALAALLARHELVLVGGWYSARLLTQSAEAEIEALQAHLALLKAMNCSVFI
                     MAETSNAIHGNQACPLAKTPRLGAAEWRDFGGKLNVVADYVARAGLSMAYHHHLGTVV
                     ESQADIERFLASTGPAVGLTVDTGHAALGGVDTLRLIREHPRRVAHVHCKDIRAAVFN
                     QVQAGAKSFLDGVLGGMFTVPGDGDIEFAKVLKALAAIHYAGWIIVEAEQDPALADPK
                     SYAALGLKTLRREAAAVHLHEADTS"
     gene            6699..>7498
                     /gene="iolB"
                     /locus_tag="VGE92_06355"
     CDS             6699..>7498
                     /gene="iolB"
                     /locus_tag="VGE92_06355"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF016826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEY1313476.1"
                     /translation="MSKLLVQSNTADATGCVLDITPQSAGWRHVGFKVQRLKPAQVAS
                     GGEAERETCLVVLQGTADVVVDGQRFEGIGGRGSVFDDAAPGAVYAPAGSHYALTARS
                     AVELAVCSAPGKPGRAPRAIAAADMSREVRGSGTNTRYVRNILPQGKEADSLLIVEVI
                     TPGGHWSSYPPHKHDTATDGKETALEETYYHRLHPTQGFAFQRVYTDERDLDETLCVH
                     DGDCVLVPRGYHPVGAAHGYDLYYLNVMAGPVRNWVFRNDPAHEWLVKP"
BASE COUNT         1280 a         2379 c         2499 g         1340 t
ORIGIN      
        1 accgcgggca gtccatcggc gccggtgacg gcgagtgccg cgcgccaggt gttgttcgca
       61 atcagctcct tgggatcggc cgaaatcgcg tgcgcgtgcg gcgcccacgg caatttcccc
      121 gcgacgtttg cgccgagcgc ccgcgcggtt gccgcaatct gcgagaggcg gtcggcggga
      181 atctcgacgc gcaattcctc gagcagcacc tcgccggtct tggggctttc gattctctgc
      241 aataattctt ggatgatgcg aaacggggtg gccgcaatgc cgctggccat tcccgaatgc
      301 acgccttcgg tcaacacgcg cacccgcaac tgaccggtga gattgccgcg cagcgacgtc
      361 gtgcaccaca cgcgcgcgta gtcgccgcac tccgcatcca ggcaaatgac cagcgatgga
      421 tcgccgatgg catcgcccaa ggcttccagg tgcgccggca gatcgatgct gccgctttcc
      481 tcggacgcct cgatcagcag cacacaacgg gcgagcggca cccgttgctg cttcaacgcg
      541 acgatggcgg tcaatgagct gaacaccgca tagccgtcgt cggcaccgcc gcgcccgtac
      601 agccggccct cgcgcatcac cggttcccag ggtgataggc cgggcgacca gccggtaaac
      661 tccggttgct tgtccaagtg accgtagagc agcacgcagc ccggaacctc gcccggcaca
      721 tcgatgagca gcagcggcgt gcgccccggc aagcgacgta tttccacgcg cattccgggc
      781 acgggctgcg cgcggcacca ctcggccatg agctcgaccg cccgctgcat gtgtccatgt
      841 tcttgccact gcggatcgaa gtgcggcgac tggttcggga ttctgatgta ggcctgcagc
      901 cgctccacaa tcgagtgctc ccaggtgtgg tcgatcgagg cacgcagctg tttgagttcc
      961 atgggcgatt ccgggtaatg caaatttaga ccgaggcgac gcggaccggc tgattgagtt
     1021 ttgcggacaa cgctgcggct tcggcgagag cgagcgccgc gatgccatcg tgatagcctg
     1081 ccagcggttt cacgcggtcg cgcaaaatag cttcgaagtg cgccatctcg cgccggtagg
     1141 cttccgcgta gcgctccagg aaaaagtttt gcagcgcatc gccggctgca ccccgttcgg
     1201 tgcacgtcgt caccgtgctc atgagcacat tgtcggcgcg caccgtgccg agcgaaccgt
     1261 aggcttcgat gcgctggtca tagccatagc ccgaacgacg cgtattgctg atgacgcaaa
     1321 gccgcccgct ctcggtgcgc aatacgatgc gggcggtgtc gacatcgccc gccgctgcga
     1381 tcgcaggatc gatgaggcag ctcgcgctcg caaagacctc ggtgggttcc tcgcccaaga
     1441 gccagcgcgc catatcgaag tcgtgaatgg ccatgtcttt gaacaggccg cccgaggtgc
     1501 ggatatagga gacggggggc ggagccgggt cgtgactgat gatggtgagg gtctcgagct
     1561 tgccgatggc ctgcgcatcg atccgtgcct tcaaaaccgc gaaatgcgga tcgaatcgcc
     1621 gattgaatcc caccaacagg gccgcctcgg ccaacggcgc accggcttgc cgcgcgcgcg
     1681 ccaaatccag gtccaagggt ttctcgcaga agatcgcttt gccggcgctc gccaccgcca
     1741 gggcttgatc caagtgtacg tgggtggcgc tcgcgataat cacccccgag accgtggcat
     1801 cgcccaagac ggcatctaag tccgcgaccc gcgctccggt ttgctgggcg agtgcctccg
     1861 cggcggcgcg gttcgggtcc accacgaatt taagcgtcaa accggcggcg gccgcggcat
     1921 tgcgggcatg aatctgtcca attcgacccg cccccacaat ggccacgttc aacattgccg
     1981 ctcccgagga tgtgatttag agattatagt tcgtttcgtt aacaggctgc tttgcgtgaa
     2041 tttttgtcta cggcgcacga cacacttgac ttgatcgcaa tcggccgctc gagcgtcgac
     2101 ttgtatggcg agcaggtcgg cggccggctg gaggacatgg ggtcattcgc caagtacgtc
     2161 ggggggagcc ccaccaacac cgcgatcgcc gcggcgcgct tgggactcaa ggccgcgctc
     2221 ctgacccgcg tcggggccga ccatatgggg cgctttatcc gcgaggagct cgagcgcgag
     2281 ggcgtggatg tgcgcggggt gcacgcggac cccgctcgct tgaccgccct cgtggtgctc
     2341 ggcattcgca atcgcgagac gtttccgctc attttctatc gcgaaaactg cgccgacatg
     2401 gcgctttgcg aacaagatct cgatgacgga ttgttggccg aggcgcgcgc ggtgttgatc
     2461 aatggcaccc acctctccac gcccggcgtg ttcgcggcga gcgtcggcgc cgcgcgtcgc
     2521 gtcaaggcga atggcggacg ggtggtgttc gacgtcgact accgtccggt actttgggga
     2581 ttgacgccca aggatctcgg tgagaaccga ttcgtcgaga actcgcaggt gaccgaacgc
     2641 ctgcaaagcg tgctgtcatt gtgcgatttg atcgtcggca ccgaagagga gctgtgcata
     2701 ctcggcggct cggtcgagct gctggcggcc attcgtaacg tgcgcgcgcg cactgcggcg
     2761 accttggtgt gcaagcgtgg cggacacggc tgcgtcgcct tcgcccagga aatccccgat
     2821 cgcctcgagc atgggctcgt cgtccccggt ttcaagatcg aggtgttgaa cgtgttaggg
     2881 gcgggcgatg cgttcatggg gggatttctg cgcggttggc tgcgcgacgc cacgctcgag
     2941 gaatgctgcc gctacggcaa cgcctgcggc gcgatcgtgg tgtcgcgcca cggctgcgcg
     3001 ccggcgatgc cgacctgggg tgagctcgag gtgctgttgt cgaggcgggc caagcatccc
     3061 accgagtcta gcatcggcta cttggatcaa gtgcactggg cgtcgacccg ccgccgcgag
     3121 tatccagaat tgaccgtgtt cgccatcgat catcgcagcc agttcgagga actcgccgag
     3181 ccttcagggc cgcacggcga acgcatcggc cggttcaaga cgctcgggct tcaagcgctc
     3241 gatcgcgtcg cgcgccgcga cccgcgcttc ggcgtgctgc tcgatgggcg ctacggattc
     3301 gatgcgctca gccaagcggc ggatatgccg tattggattg gccggcccat cgaggtgccg
     3361 aagtcgcggc cgctcgaatt cgagggctcc gcggacgtcg cgaccgagat tgcgacctgg
     3421 ccgctcaatc aggtggtcaa gtgcctggtg atctatcacc cggacgatgc ggcggagttg
     3481 cgcgagcggc aggaacgcca gttgctgcgc ctgttcgacg cctgccgcaa gacgcgtcac
     3541 gagttgttgc tcgagatcat cgcaccggcg gggacccggg cggatgcgcg caccacggcg
     3601 cgcgcgatcg aggagatcta tgcgcgcggc atttaccccg actggtggaa gcttgcgccc
     3661 ggcagcaagg cccattggca ggccattgaa gagacgatca tgcgcgagga tccccgctgt
     3721 cgcggtgtcg tgctcttagg tctcggggcg agcgcggcgg acctcagcgc cgcgttcgcg
     3781 actgccgcgc cgtttgccat cgtcaaaggg ttcgcgatcg gtcgaagcgt gtttcaggac
     3841 accgcccgag cctggttcgc gaattcgctg agcgatggcg atgcgtgcca ggcgatggcc
     3901 gaccaattgt cgaccttggt gagcgcgtgg cgcggcgcgc gtcaggcact tgcctcatga
     3961 aaaccatccg ctgcaccgcc gcgcaagcca ccgtgcgcta tcttgcgaat cagtacatcg
     4021 ccggcgcttc cggtgacgtc ccgtatttcg cgggggtgtg ggccattctg ggacacggca
     4081 atgtggcggg gctgggtgag gcgctgtacg ccgcacagaa gcaaattccc acttaccgcg
     4141 cgcacaacga gcagggcatg gcgctcgccg cgattgcctt tgcgaagcag ctgcgacggc
     4201 gccgcgccat gatttgcacg agttcgatcg gccccggcgc caccaacatg gtgaccgccg
     4261 cggcgttggc gcacgtgaat cgattgccgg tgctgtttct gccgggcgat gtgtttgcct
     4321 cgcgcggccc ggacccggtg ctgcagcaga tcgagagttt tgccgacggc accgtgtccg
     4381 ccaacgattg ctttcgaccg gtgtcgcgtt atttcgaccg cattgcgcgc gccgaacaaa
     4441 tcatcgacgc attgccgcgc gccatgaata cgctgctcga ttcggcctcc tgcggaccgg
     4501 tgacgctcgc cttctgccag gacacgcaag ccgaggccta tccctatccg gaagctttct
     4561 ttcggcgccg ggaatggagc attcgacgtc cgccgggcga tcgccgcgag ctcgtgcaac
     4621 tggtcacgct gatgcgctcg gcgcgcacgc cgttgatcat cgcgggcggc ggcgttcact
     4681 atagcggcgc gcaggacgtg ctcgcggcct ttgcgagcag cacccggatt ccggtcgcgg
     4741 agacgcaggc cgggaaaggc gccttggcct ggaatcatcc cttcaatttg ggcgccatcg
     4801 gtgtcaccgg cacgagcgcg gccaacgagg cggcggcgcg cgcggatctc gtgatcggca
     4861 tcggcacccg attacaggat ttcaccacgg gttcgcgcgc gctctttacc gcgccgaatc
     4921 gccgtttggt acaaatcaac atcgccgcct acgatgccgg caagcacgag gcatcgacca
     4981 tggtcggcga cgccggggcc gtgttgtcgg aacttgcgcg gcgcatgggc gactggcgca
     5041 gcgccaacaa cgaggagctc atccgcgcgg acgtggcgca ttggaacaac gtctggactc
     5101 aagccacggc ggccggcaac gcgcttccct ccgacgccca agtgatcggc gccctgtggc
     5161 gaaattcccc ggacaacgcc gtggtcgtgt gcgccgcggg cggattgccg ggggaactgc
     5221 acaagctgtg gcgctcgcgc gagcgcgacg actaccatgc cgaatatggt tattcctgca
     5281 tggggtacga gattgccggg ggccttggcg tcaagatggc gcagccgcag cgcgaggtgt
     5341 acgtcatggt gggcgatggc agctatctca tgctcaactc cgagctcgcc acctccgtca
     5401 ccttggggtt caaactggtg gtgatcgtgc tcaacaaccg cggatttggc tgcatcagtc
     5461 gtctgcagcg ctcgaccggc gcggccgcct tcaacaattt gtgggagccg gacgcgcgcg
     5521 gcgccgagat cgattttgtc gcgcacgcgg cgagtctcgg tgccatcgcc gaaaaggccg
     5581 actcgattca ggggctggaa ctcgcactgc aacgcgcgcg cgagtcgacg cgcagccacg
     5641 tgatcgtgat cgacaccgat cccatcaaga ccacggcgga aggcggggcg tggtgggatg
     5701 tggcaatccc cgaggtttcc tcgcgccacg aggtcgatgc cgcacgcacg gcctcgctgc
     5761 gagagcgcaa gaatcggcgc ttgggagatt agccgcatga gcattcgttt cggtgtcagt
     5821 cccatcgcgt ggatcaacga tgatctaccg gcgctcggcg gcgacacgcc actcgattcg
     5881 gtgctcgccg atgcgcacga agtgggattc gcaggcatcg aactcggcgg aaaattcccg
     5941 aaggagccgc gcgcgctcgc ggcattgctc gcccggcacg agctcgtctt ggtcggcggc
     6001 tggtacagcg cccgcttgtt gacgcagtcg gccgaggcgg agatcgaggc gctgcaggcg
     6061 cacctcgcgc tgttgaaggc gatgaattgc agcgtattca tcatggcgga gacgagtaat
     6121 gccatccacg gcaatcaggc gtgtccgctc gcgaaaaccc cgcggctcgg cgccgcggag
     6181 tggcgcgatt tcggcgggaa actcaacgtc gtcgccgact acgtcgcgcg ggcggggctc
     6241 tccatggcgt atcaccatca tttggggacg gtcgtggaat cgcaagctga tatcgagcgt
     6301 tttctcgcct ccacgggccc cgcggtcggt ctcacggtgg acacggggca cgcggcactc
     6361 ggcggcgtcg acacgttgcg cctgattcgc gagcacccgc ggcgcgtcgc gcacgtgcat
     6421 tgcaaggaca tccgcgccgc cgtcttcaat caagtgcagg cgggcgccaa aagttttttg
     6481 gatggcgtgc tcggcggcat gttcacggta ccgggcgatg gcgatatcga attcgccaag
     6541 gtccttaagg cgctcgctgc gattcactat gcgggttgga tcatcgtcga ggcggaacaa
     6601 gacccggcgc tcgccgatcc aaagtcctat gcggcgcttg gactcaagac cctgcgccgt
     6661 gaggcagcgg ccgtccattt gcacgaggca gacacttcat gagtaagctc ctggttcagt
     6721 cgaacacagc ggatgccacg ggctgtgtgc tcgacatcac gccgcagagc gccggctggc
     6781 gccatgtggg attcaaagta cagcggctga aacccgctca ggtggcgagc ggcggcgagg
     6841 ccgagcgcga aacctgcctc gtggtgctgc aagggacggc ggatgtggtc gttgacgggc
     6901 aacgcttcga gggcatcggc ggtcgcggct cggtcttcga tgacgcagca ccgggcgcgg
     6961 tgtacgcgcc ggccggttcg cactatgcgc tgacggcacg ctcggcggtg gaattggcgg
     7021 tttgctcggc cccgggcaaa cccggacgcg cgccgcgcgc gattgccgct gccgatatga
     7081 gccgcgaagt gcgcggcagc ggcacgaaca cccgctacgt gagaaatatt cttccacaag
     7141 gcaaggaggc cgacagcttg cttattgtcg aggtcatcac gcccggcggc cattggtcga
     7201 gctacccgcc gcataaacac gacaccgcga ccgacggcaa ggaaaccgcg ctcgaggaaa
     7261 cctactacca tcgcttgcac ccgacgcagg ggtttgcctt ccagcgcgtg tataccgacg
     7321 aacgcgatct cgatgaaacc ttgtgcgtgc acgacgggga ttgcgtgctc gtgccgcggg
     7381 gctaccaccc ggtgggcgcc gcccatggat atgatctcta ttatctcaat gtcatggcgg
     7441 gtccggtgcg caactgggtg tttcgcaacg atcccgcaca tgagtggctg gtgaagcc
//
LOCUS       DASWZT010000231        13710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_11545, whole genome shotgun sequence.
ACCESSION   DASWZT010000231 DASWZT010000000
VERSION     DASWZT010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13710
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11545"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /locus_tag="VGE92_06360"
     CDS             complement(<1..1225)
                     /locus_tag="VGE92_06360"
                     /inference="COORDINATES: protein motif:HMM:NF015764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HEY1313477.1"
                     /translation="MRIQTRLCAMMFLQFFIWGGWFVTMGSFLAANLGASGAQIGVAY
                     STQSWGAILAPFIVGLMADRFFNAERLLSVLHLAGGALMFALARSSHFTSFYPCLLAY
                     MILYMPTLALVNSISFRQLADPSKQFAKIRVWGTIGWIVVGLMISFVFSWDARASIAA
                     GMLKNTFMLCGVASVVLGLYSLTLPSTPPGGKTGQPVRLRDIVGLDALTLLNNRNFLV
                     FFISSILICIPLAFYYQHANQFLTELHVANPTGKQTIGQMSEVLFMLLIPLFLNRFGM
                     KTTLLVGMFAWAARYVLFAFGNANGLVALLLVGIALHGVCYDFFFVSGQIFIDTKAGK
                     EIKSAAQGLITLATYGVGMLIGFWAAGLITDMYPGVGGVHQWQTIWLYPAAFAALIFA
                     FFALSFKNENVKYDIQ"
     gene            complement(1239..2318)
                     /locus_tag="VGE92_06365"
     CDS             complement(1239..2318)
                     /locus_tag="VGE92_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313478.1"
                     /translation="MTISRRAFLEATVAATSALAFSAGTVGGAQAAEPKHKIKRGVAM
                     YSFQEEYFMRTMTVEDCLRQMSDIGAYRIELLAEMMVPNFPNPSDAWVDQWHGWVDKY
                     KMVPTAYTQFIDTMRTKTHNLTVEEGVQTMMRDIKLAKRLGIPKIRALVGTPVDILEA
                     TVPQLEKENVWLGVEIHFPIPIKGHLVERLLKIADKTDHFGFVPDFGIFQNKPNPYQR
                     DRMVRDGDLTHDAALFIENAWENKVDKQKVIADVKKMSAGPGAQGYVENVYGIVAQDP
                     RDLLPIMSKCRHIHGKTWGLTEACVDPAIDLTQVIPTLIKGGYDGDIATEYEGQRWVQ
                     DVEPFSAVEMIDRHQVMLRRLLGEI"
     gene            2401..2868
                     /locus_tag="VGE92_06370"
     CDS             2401..2868
                     /locus_tag="VGE92_06370"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY1313479.1"
                     /translation="MRHKVITGTLILGLSTAMLACGAHPQPNPLGLRPHHITASVLDL
                     ERAVNWYRSMLGFEVKERGSHGTMQFAELAVPGFGVALVKEAGIDPRDPLARQAPPYW
                     MHMVFSVADPDAVYGLLKSRGALVSTRQEPPPRPISSFLIKDSEGNEIEIVKH"
     gene            complement(2879..3340)
                     /locus_tag="VGE92_06375"
     CDS             complement(2879..3340)
                     /locus_tag="VGE92_06375"
                     /inference="COORDINATES: protein motif:HMM:NF040396.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6379 domain-containing protein"
                     /protein_id="HEY1313480.1"
                     /translation="MQDRYVIMNTGFKNIVEAGAIAGYQLNIREPYYRGTFLSSVHYF
                     DLAVDGAKVPRENIKIMVAGKTFTLDQMEEADNVRWQFGDAATLLIRKAGGLTPGIHE
                     IRVGIVIRKSYLPPSDPEHLYSDFPGLWRDGKYSTYIEAPSFITKKMTLVQ"
     gene            3532..4218
                     /locus_tag="VGE92_06380"
     CDS             3532..4218
                     /locus_tag="VGE92_06380"
                     /inference="COORDINATES: protein motif:HMM:NF036707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313481.1"
                     /translation="MELSEVSALLQPAPMSLETGYERFANGVLHVACRTDLHGCSGAM
                     FEWWFRSRPDTQQYIWWHPIDHVSSHWREARDGTPVGSIHIVEEYFSGLPSQKLSIQF
                     REPGEFFDAGAYTQARTERRVSAAICACIAASHEPQRGADGKILGGRLLHIGRDTPWG
                     CALRSHFYLGQDLPGLGLDAAKIEEIFPDVFGRALLQHCYDEFTFLSRFLPSLYIAEN
                     RSTEKITPPW"
     gene            complement(4225..5661)
                     /locus_tag="VGE92_06385"
     CDS             complement(4225..5661)
                     /locus_tag="VGE92_06385"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF006943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HEY1313482.1"
                     /translation="MSHDVKSAERPAPDPILTAIADYALSFEIKSKLAYETAGYCLMD
                     TLACGFQALKYPACTKLLGPVVPGATLTNGARVPGTAYELDPINAAFNIGAMIRWLDF
                     NDTWLAAEWGHPSDNLGGILAVADYLSRSGKAVSVRDVLTAMIKAHEIQGVLALENSF
                     NRVGLDHVLLVRVASTAVVTAMLGGSRQQIIDAVSNAWVDGGALRTYRHAPNTGSRKS
                     WAAGDATSRAVRHALFALKGEMGYPSALSAKTWGFQDVLFKGKALSLPQPFGSYVMEN
                     VLFKISYPAEFHAQTAVEAAMTLHGQIAAKLGAIERVTIETQEPGVRIIDKTGALANP
                     ADRDHCIQYMVAIPLLFGRLTAADYEDQIANDPRVDALRAKMQVRENATFTQEYYDPN
                     KRYIGNAVQVFFKDGSASERVQVDYPIGHRKRREEGMPVLVRKFEASVAAHFGPKQTE
                     SITALFVDRAALAAMPVSEFVANLVTSV"
     gene            complement(5679..6839)
                     /gene="prpC"
                     /locus_tag="VGE92_06390"
     CDS             complement(5679..6839)
                     /gene="prpC"
                     /locus_tag="VGE92_06390"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF009006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEY1313483.1"
                     /translation="MPEAQSQIAAFKPKKSVALSGVTAGNTALCTVGRTGNDLHYRGY
                     DILDIAAECEFEEIAYLLVHEKLPNRAELAAYKSKLQSLRALPAAVATVLEQLPAEAH
                     PMDVLRTGVSALGCCIPEAGAHTNAGARDIADRLLASLGSMLCYWYHYAREGRRIEVR
                     TDDDSIGAHFLHLLHGRRPCASWVRAMHTSLNLYAEHEFNASTFTARVIAGTGSDVYS
                     CITGAIGALRGPKHGGANEAAFEIQNRYETPDAAAADIKRRVAAKEVIIGFGHPVYTI
                     ADPRNQVIKSVALNLAREANDLRGFEVAERIESLMHEIKNMFPNLDWFSAVSYHLMGV
                     PTAMFTPLFVISRTSGWAAHVIEQREDGKIIRPSANYTGPENVPFVPLARRP"
     gene            complement(6859..7752)
                     /gene="prpB"
                     /locus_tag="VGE92_06395"
     CDS             complement(6859..7752)
                     /gene="prpB"
                     /locus_tag="VGE92_06395"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016142557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEY1313484.1"
                     /translation="MTTRVASPGALFRQAVAAERPLQVVGAINAYAARMAAACGFKAL
                     YLSGGGVAANSLGVPDLGISTMDDVLIDIRRITDATPLPLLVDADTGWGGAFNIARTV
                     KSFIKFGAAGLHIEDQVQAKRCGHRPGKEIVSKQEMVDRVKAAVDARSDPGFVIMART
                     DALAVEGIDKAIERAVACVEAGADMIFPEAMTELPMYARFKEAVGVPVLANITEFGHT
                     PLYTREELGSVGVDIVLHCCSAYRAMNAAALATYQAIRRDGTQKNVLDLMQTRADLYK
                     YLDYHAYEAKLDELFSVGKEK"
     gene            complement(7749..8441)
                     /locus_tag="VGE92_06400"
     CDS             complement(7749..8441)
                     /locus_tag="VGE92_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY1313485.1"
                     /translation="MNLAGKILAVTGSNGALGQAVAVTLSGYGARLALLDHAREVSVA
                     VPAGALHYGGVDLSDADAARAAMQRIIKETGRLDGLINLAGGFQWQKLAGGSLDSWDA
                     MYKLNLRTAVSSCQAALPYLLQAGGGRIVNVGAMGAVKAAAGMGAYAASKAGVAQLTQ
                     ALADELKDRGITVNAILPSTLDTPRNRLDMPDADFTRWVAPSEAAEVIAFLVSNEARA
                     ITGALIPVAGRV"
     gene            complement(8538..9614)
                     /locus_tag="VGE92_06405"
     CDS             complement(8538..9614)
                     /locus_tag="VGE92_06405"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY1313486.1"
                     /translation="MRQRNGNTELSPTRGTAGTATRSPEPGTIAICFVAAALQSVRAR
                     DLNADELLANVGLSSSLLQVPQARVSAKHYGALWRSIALALDDEFFGQDSRRMKAGSF
                     AMLCHSVLNCKTLGQALERSLRFYALILDDISGTLERNSKEARIVLRERAAGASQRIF
                     AHELLLMLLYGVSCWLIGRRIPILRTEFSYAEPGHSAEYRLMYCADLSFDKPNTLLAF
                     DSSYLDLPVVQDERSLKEFLRTAPESILLKYKNGSSLTARVRRRLRQFLPGEVPDFEQ
                     LASDLNMTAATMRRRLHEEGESYQSIKDQLRRDLAIGYLSHTTRSVMDIALELGFSER
                     SAFHRAFRKWTGASPGEFRRTVQA"
     gene            9715..11241
                     /locus_tag="VGE92_06410"
     CDS             9715..11241
                     /locus_tag="VGE92_06410"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY1313487.1"
                     /translation="MTIQPATLVKPPVTVKLLLDGKFVESRSAEWHDVVNPATQEVLA
                     QVPFATAEEIEAAINSAKHAYKTWKNTPLATRARIMLKLQALVRDHMSRIAQTLSAEQ
                     GKTLADAEGDVFRGLEVVEHACSIGSLQLGEFGENVAGGVDTYTLRQPIGVCAGISPF
                     NFPAMIPLWMFPMAIVCGNTFVLKPSEQDPLTPMLLAELALEAGVPPGVLNIVHGGKR
                     AVDALCTHPDIAAVSFVGSTAVGTHVYNLASQSGKRVQCMMGAKNHAVVMPDANKEQS
                     LNSLVGATFGAAGQRCMATSVTVLVGEARKWIPDIVAKAKTLKVNAGVEKGADLGPVV
                     SKNAKQRILGLLEDGIKEGAKLELDGRSIKVPGYEKGNFIGPTIFSAVTPAMKIYTTE
                     IFGPVMIIVSVDTLDEAISLINNNPFGNGTGIFTQSGAVARKFQSEIDVGQVGINVPI
                     PVPVPVFSFTGSRGSKLGDLGPYGKQAVQFYTQTKTVTSRWFDDSSTPGAVNTTISLK
                     "
     gene            11251..12333
                     /locus_tag="VGE92_06415"
     CDS             11251..12333
                     /locus_tag="VGE92_06415"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF004127.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY1313488.1"
                     /translation="MEVLSEIRNHVAFITLNRPAALNALSLEMLLRLRTLLSESASDP
                     QVHAVVLQGAGDRAFCAGGDIRALYQSFKSTGTLHRQFFLTEYPLDYALYAYPKPYVV
                     VMDGITMGGGMGIAQGSAMRVVGERTRIAMPEVAIGFFPDVGASYFLSRLPGALGIYL
                     ALTGVQIRGADALYCHLADVYLTPQAIAALMDDLSTLDWSDDCANDLRRFIHARAAQG
                     LAAPSLSRLRTGIDAHFVHPTVRAILASLDAETNAEYTDWARQTAALMRSRSPTMLCV
                     TLRQLQRGKTMTLAACFRMELSMVQQCFEQGDFIEGVRALLIDRDNAPHWTPNRVEDV
                     TDGMVDTFFRSRWTAAGHPLSILQGF"
     gene            12343..13110
                     /locus_tag="VGE92_06420"
     CDS             12343..13110
                     /locus_tag="VGE92_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004278772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY1313489.1"
                     /translation="MQLKDTHAVITGGASGLGLAVAKRVILAGGKATLLDVNAAQGAA
                     AAAALGERASFIATDVTDESAVDGAIDRARERLGAIQAAVNCAGIATPTRLVGKQGAM
                     SGAYFRKMIEINLIGTLLVAKAAAVAMQLNSPNAEGERGVIIMTASVAAFDGQIGQVA
                     YAASKGGIVGMTLPMARDMASSGIRVMTIAPGLFATPMMKGLPQEAQDSLGKQTPFPP
                     RLGEPDEYAALVGHIIENVMLDGETIRLDGAIRMAPR"
     gene            complement(13124..13330)
                     /locus_tag="VGE92_06425"
     CDS             complement(13124..13330)
                     /locus_tag="VGE92_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003157722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HEY1313490.1"
                     /translation="MGGASLMAAAVPSPCIDVCRLDTQGLCVGCRRTLNEIAEWSTAG
                     EARQREILRDVARRKELVGQVPGR"
     gene            complement(13312..>13710)
                     /locus_tag="VGE92_06430"
     CDS             complement(13312..>13710)
                     /locus_tag="VGE92_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014596135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY1313491.1"
                     /translation="AAGMSRRPRVYFEEWDQPLITGIRWVSELIGIAGGDDVFPERAV
                     CSLGKDRILSDANEVIRRAPDIIIGSWCGKRFRPEQVAARLGWNAVPAVRDGELHEIK
                     SPLILQPGPAALTDGLDAIHRLLREWAAHR"
BASE COUNT         2706 a         4259 c         4086 g         2659 t
ORIGIN      
        1 attggatgtc gtacttgacg ttctcatttt tgaacgacag tgcgaagaac gcgaatatca
       61 aggccgcaaa ggcagccgga taaagccaga tcgtttgcca ttgatgcacg ccgccgaccc
      121 ccgggtacat atcggtgatc agtccggcgg cccagaatcc gatgagcatt ccgacgccat
      181 acgttgccag cgtgatcaat ccctgcgcag cgctcttgat ttccttgccg gccttggtat
      241 cgatgaagat ctggcccgac acgaagaaaa aatcgtagca cacgccgtgc agcgcaattc
      301 cgaccaatag caaggcgacc aggccgttcg cgttgccgaa cgcaaacagc acatagcgag
      361 cggcccatgc gaacattccc accagcagcg tggtcttcat gccgaagcga ttcaaaaaca
      421 gtggaatcaa cagcatgaac aatacctccg acatttggcc gatggtttgt ttgccggtcg
      481 ggttggcaac gtgcaattcg gtgaggaatt gattggcatg ctggtaatag aacgccagcg
      541 gtatgcaaat caggattgac gagatgaaga aaacgaggaa attccgattg ttgagcaggg
      601 taagcgcgtc gagaccgaca atatcgcgca gccgaaccgg ttggcccgtc ttgccgcccg
      661 gcggcgtgct cggcagcgtc aggctataga gtccgagcac cacggaagct acgccgcaca
      721 acatgaacgt attctttagc atgccggcgg cgatgctggc gcgcgcatcc caggaaaaca
      781 cgaagctgat catcaagccg acgacgatcc aaccaatcgt tccccacacg cggattttcg
      841 cgaactgctt cgacggatcg gcgagctgac ggaacgaaat cgagttcacc agcgcaagcg
      901 tcggcatata caggatcatg taagcgagca agcacgggta gaacgatgtg aagtggctgg
      961 agcgcgcgag cgcaaacatc aaggcgccgc ccgccaaatg cagaaccgac agcaaccgtt
     1021 cggcgttgaa gaagcgatcc gccatcagac ccacgatgaa aggcgcaaga atcgcgcccc
     1081 acgactgagt gctgtaggca accccgatct gcgcgccgct cgcgcccagg ttggccgcca
     1141 ggaagctgcc catggtgacg aaccagccgc cccagatgaa gaattggaga aacatcattg
     1201 cgcacagccg agtctgaata cgcacggtgc cgggatacct agatttcgcc caataggcgc
     1261 cgcaacatca cctgatgacg gtcgatcatc tccaccgcgc taaacggttc gacatcctgc
     1321 acccaacgct gcccctcgta ttcggtcgca atgtcaccat catatccccc cttgatcaag
     1381 gtcggtatga cttgagtcag atcgatcgct gggtcgacac aggcttcggt caagccccac
     1441 gtcttgccgt gaatgtgccg gcacttcgac ataatcggca gcagatcgcg gggatcctgc
     1501 gcgacgatgc cgtacacgtt ctccacgtag ccttgcgcgc cggggcctgc cgacatcttt
     1561 ttgacgtcgg cgattacttt ttgcttgtcg actttgttct cccaggcatt ttcgatgaac
     1621 aaggccgcat cgtgggtaag atctccgtcg cgcaccatgc gatcgcgctg gtaggggttg
     1681 ggtttgttct gaaagatccc gaaatccgga acgaatccga agtgatcggt cttgtccgca
     1741 attttcaaca gacgctccac caagtgtcct ttgatcggaa tcgggaagtg gatctccacc
     1801 ccgagccaca cattttcctt ttccaattgc ggcaccgtcg cctcgagaat gtcgaccggc
     1861 gtgcccacca gcgcgcgaat tttcggaata ccgaggcgct tggccagctt gatgtcccgc
     1921 atcatggtct ggacaccctc ctcgaccgtc aaattgtgcg tcttggtgcg catcgtatcg
     1981 atgaattgcg tataggcggt cggcaccatc ttgtacttat cgacccaacc gtgccattga
     2041 tcgacccagg cgtcgctcgg attcgggaaa ttcggaacca tcatttccgc aagcagttcg
     2101 atgcggtagg cgccaatatc cgacatctgc cgcaggcaat cctcgaccgt catggtccgc
     2161 atgaagtact cttcctgaaa ggagtacatc gccacgccgc gcttgatctt gtgcttgggc
     2221 tcggccgcct gcgcgccgcc cactgttccg gccgagaacg ccaacgcgga cgtcgccgcc
     2281 acggttgcct cgagaaatgc tctgcgtgaa atggtcatct caattccgtc cgtacgtatg
     2341 gttggatagg tcaattcgat tgtaatgcgt tacatccgct gctcggctcc gaggtccacg
     2401 atgcgacaca aggttattac cggcactctg atcttggggt tgtccaccgc catgctggcg
     2461 tgcggcgcac acccgcagcc gaatcccctg ggcctgcggc cgcaccacat taccgcctcg
     2521 gtgctcgatc tggagcgcgc cgtgaactgg taccgaagca tgttgggctt cgaagtcaag
     2581 gaacgcggca gtcatggcac gatgcagttc gcggaattgg cggttcccgg ctttggagtc
     2641 gccctcgtca aggaagccgg gatcgatccg cgggatccgc tcgcccgtca ggcgccgccc
     2701 tattggatgc acatggtatt ttcggttgcg gatccggatg cggtgtatgg tcttctgaag
     2761 agtcgcgggg cgctcgtcag tactcgccaa gaaccgccgc cacgacccat atcgagcttc
     2821 ctgatcaagg acagcgaagg caacgagatc gaaatcgtga agcactagtc gtgcacgatt
     2881 attgaacgag agtcatcttt ttcgtgatga acgacggcgc ttcgatgtac gtcgaatact
     2941 tgccgtcgcg ccacagcccc gggaaatccg aatacaaatg ctcggggtcg gatggcggca
     3001 ggtaggattt tcgaatgacg atgccgaccc ggatctcgtg aattcccggc gtcaagccgc
     3061 cggctttgcg tatcagcagc gtcgcggcat cgccaaactg ccagcgcacg ttgtcggcct
     3121 cctccatctg atcgagcgta aaggtcttgc cggcgaccat gatcttgatg ttctcgcgtg
     3181 gcactttcgc tccatcgacc gccaagtcga agtaatgcac cgacgacaag aatgtgccgc
     3241 gatagtacgg ttcgcgaatg ttgagttggt aaccggcgat cgcccccgct tcgacgatgt
     3301 tcttgaagcc ggtattcatg atgacgtatc ggtcttgcat cgaaattccc cccccgaaac
     3361 cggccgtttt ccgcgactcg tggaattaga ctcttgagcg ggccgaagct ggcgctgatg
     3421 taatcgatat cctaagcgtt tcggcgcgcg ccggtcaaca catttgtaat gcgttacatt
     3481 tgcgctatca tgcgccgggc tgaattgact gcggctgcag aggggggtgt catggagtta
     3541 tcggaagtct cggcgctgtt gcaacccgcg ccaatgtcct tggaaaccgg ctacgaacga
     3601 ttcgccaatg gcgtgctgca cgtcgcctgc cgcaccgatc tgcacggctg cagcggcgcg
     3661 atgttcgaat ggtggtttcg atcccgcccg gacacccagc aatacatctg gtggcatccc
     3721 atcgatcatg tgtcgagcca ctggcgcgag gcgcgcgacg gcaccccggt aggttccatt
     3781 cacatcgtcg aggagtattt ctcgggcttg ccgtcgcaga aactgtccat tcaatttcgc
     3841 gagcccggcg aattcttcga cgccggggcc tatacgcaag cgcggaccga gcggcgcgtt
     3901 tcggcggcca tttgcgcgtg catcgccgca tcccacgagc cgcaacgcgg cgcggacgga
     3961 aagatcctcg gcggccggct gctgcacatc ggcagagata cgccctgggg ctgcgccctg
     4021 cgcagtcact tctacttggg tcaagacttg cccggccttg gcttggatgc cgcgaagatc
     4081 gaggaaattt tccccgacgt cttcggcagg gccttgctgc agcactgcta tgacgagttt
     4141 acctttttgt cccgtttcct gccgtcgctg tacattgcgg agaatcgcag cacagagaaa
     4201 atcacgccgc cctggtgatg cccgctaaac cgaggtcacc agattcgcca cgaactcgct
     4261 gacaggcatc gcggcgagcg cggcccggtc gacgaaaagc gccgtgatcg actcggtttg
     4321 cttggggcca aaatgcgccg cgacgctcgc ttcgaatttc ctcaccagta ccggcatgcc
     4381 ctcctcgcgc cgcttgcgat gcccgatcgg gtaatccact tgcacgcgct cgctcgcgga
     4441 accgtccttg aagaacactt gcaccgcgtt gccgatgtag cgtttgttgg ggtcgtagta
     4501 ctcctgcgtg aaggttgcgt tctcacggac ctgcattttg gcgcgcagcg catcgacgcg
     4561 cggatcgttg gcaatttgat cttcgtaatc cgcggcggtc aacctgccga acagcagcgg
     4621 aatcgcgacc atgtattgga tgcaatgatc gcggtccgcg ggattggcga gcgcgccggt
     4681 cttgtcgatg atgcgcaccc ccggttcctg ggtttcaatc gtcacgcgct cgattgcgcc
     4741 gagttttgcg gcgatttgcc cgtgcaaggt catggccgct tcgacggccg tctgcgcgtg
     4801 gaattcggcc gggtacgaga tcttgaacag cacattctcc atgacatagc tgccaaaggg
     4861 ctgcggcaag gacaacgcct tgcccttgaa caacacgtcc tggaaacccc aggtcttcgc
     4921 cgacaatgcc gacggatagc ccatctcgcc cttcagcgcg aacagtgcgt gacgcaccgc
     4981 gcggctggtc gcatcgcccg ccgcccagct cttgcgtgaa ccggtgtttg gtgcatgtcg
     5041 gtaggtgcgc aatgcgccgc catccaccca tgcgttcgac accgcgtcga tgatttgctg
     5101 gcgcgagccg ccgagcatcg cggtgacgac ggcggtggac gccacccgca ccagcaatac
     5161 atgatcgagc ccgacccgat tgaaactatt ctcgagcgca agaacgccct gaatttcgtg
     5221 cgccttgatc atggcggtga gcacgtcgcg caccgacacg gctttgccgg atcgcgacag
     5281 gtagtcggca acggcaagaa tgccgccgag attgtcggac gggtgccccc actctgcggc
     5341 gagccacgtg tcattgaagt cgagccagcg aatcatggcg ccgatgttga aagcggcatt
     5401 gatcggatcg agttcatacg ccgtgccagg cacgcgcgcg ccgttcgtga gggtggcccc
     5461 gggaaccacg ggccccagca gtttcgtgca cgccggatac ttcaatgcct gaaagccgca
     5521 cgccaaggtg tccatgaggc aatagcctgc ggtttcgtag gcgagtttgc tcttgatctc
     5581 gaagctcaat gcatagtcgg caattgccgt gagaatcgga tccggcgcgg gccgttctgc
     5641 ggatttgacg tcgtgagaca tgggacaact aactccgatt atggccggcg cgcaagcggc
     5701 acgaacggta cgttctccgg gccggtgtaa ttcgcgctcg gacgaatgat cttgccgtcc
     5761 tcgcgctgtt cgatgacatg tgcggcccag cccgatgtac ggctgatcac gaacagcggc
     5821 gtgaacatcg cggtcggcac acccatcagg tgataggaca ccgcggagaa ccagtcgaga
     5881 ttcggaaaca tattcttgat ctcgtgcatc agcgactcga ttcgctcagc gacctcgaat
     5941 ccgcgcaggt cgttggcttc gcgcgcgagg ttcaaggcaa cgcttttgat gacttgattt
     6001 cgcggatccg cgatcgtgta gacgggatgg ccgaaaccga tgatgacctc tttggcggcg
     6061 acgcgccgct tgatatccgc agcggcggca tccggcgtct catagcggtt ttggatctcg
     6121 aaggccgcct cgttcgcgcc gccgtgcttc gggccgcgca gcgcgccgat ggcgccggta
     6181 atgcaggaat acacatccga gccggtcccc gcaatcacgc gtgccgtaaa ggtcgaggcg
     6241 ttgaattcgt gctcggcgta cagattgagc gaggtatgca tcgcgcgcac ccatgaggcg
     6301 caaggcctgc gtccatgcag caagtgcaag aagtgagcgc cgatcgaatc gtcgtcggtg
     6361 cgcacctcga tgcgccgtcc ttcgcgcgcg tagtgatacc aatagcaaag catggaaccg
     6421 agcgaggcca gcagccggtc ggcgatgtcg cgcgccccgg cattggtgtg agcgcccgct
     6481 tcgggaatgc agcagccgag cgccgagacg ccggtacgca ggacgtccat cggatgagcc
     6541 tcggccggca gctgttcgag cacggtggcg accgcggccg gtaatgcacg cagcgactgc
     6601 agcttggatt tgtaagcagc aagttcagcg cggttcggca gcttttcgtg gaccagcagg
     6661 taggcgattt cttcgaactc gcactcggcg gcaatgtcca gaatatcgta gccgcgatag
     6721 tgcaagtcat tgccggttct accgacggtg cacaatgccg tattgcccgc cgttacaccc
     6781 gacaatgcca cggatttttt tggtttgaat gcggcgatct ggctttgagc ttcgggcatg
     6841 gctatctcct taaatccgct acttttcttt gccgacggaa aataactcgt ccagtttggc
     6901 ttcgtatgcg tggtagtcga ggtatttgta cagatctgcg cgcgtctgca tgaggtcaag
     6961 cacgttcttt tgggtgccgt cgcggcgaat tgcctgatag gttgcgagtg ctgcggcatt
     7021 catcgcccga tatgcggagc agcaatgcag cacgatgtcg acgccgaccg atcccagttc
     7081 ctcgcgcgta tacagcggtg tgtgaccaaa ttcggtgatg ttggcgagaa ccggcacgcc
     7141 gacggcctcc ttgaaccgcg cgtacatggg aagttcggtc atcgcttccg ggaaaatcat
     7201 gtcggcgccc gcttcgacgc aggcgaccgc gcgctcgatc gccttgtcga tgccttccac
     7261 cgccaatgca tcggtgcgcg ccatgatcac gaatccggga tcgctgcgcg catcgactgc
     7321 tgctttcacc cggtccacca tctcttgctt cgagacgatc tccttgccgg gcctgtgacc
     7381 gcaacgcttg gcttggacct ggtcttcgat atgcaaaccg gcggcaccaa acttgatgaa
     7441 ggatttgacg gtgcgcgcaa tattgaaggc gccgccccat ccggtgtcgg cgtccaccaa
     7501 tagcggcagc ggcgttgcat ccgtgatgcg gcgtatgtcg atcagcacat cgtccatggt
     7561 gctgatcccg agatcgggaa cgcccaagga gttggcggcc acgccgcctc ccgataggta
     7621 cagggctttg aacccacagg cggccgccat tctcgcggcg taggcattga tggctccgac
     7681 cacttgcaag ggacgctcgg cggcaaccgc ctgacggaac aacgcgcccg gcgatgcgac
     7741 tcgggtcgtc atacccgacc cgccaccggg atcaaagcgc cggtaattgc acgagcctcg
     7801 ttcgaaacca gaaacgcgat cacttcggca gcctccgatg gagcgaccca gcgagtgaag
     7861 tcggcgtccg gcatatccag gcgattcctc ggcgtatcga gcgtactcgg caatatggcg
     7921 ttgaccgtta tgccgcggtc cttcagttcg tcggccagag cctgcgtgag ttgcgcgacg
     7981 cccgccttgg aagcggcata ggctcccatt ccggcggcgg ccttcacggc tcccatggcg
     8041 ccgacattga cgatgcgtcc accgccggcc tgcagcagat acggcaaggc ggcctgacaa
     8101 gacgacaccg cggttcgcaa attcagcttg tacatggcgt cccagctgtc gagcgaccct
     8161 ccggcaagct tctgccactg gaaaccgccg gccagattga tcaggccatc gagacggccg
     8221 gtctccttga tgatccgttg catcgcggcg cgtgcggcgt cagcgtcgct cagatcgacc
     8281 ccgccgtaat gcaatgcacc ggccggcaca gcgaccgaca cctcacgcgc gtgatcgagc
     8341 agcgcgagcc tcgcgccgta gccgctcaat gtcacggcga ccgcctgacc taaagcaccg
     8401 ttactcccgg tcaccgccaa gatcttgcct gccaagttca tttctaaatt atcctcgatt
     8461 cgtcgctgca accagagtga ccaaaccgct caaaatgcct gagcggaaat gccacacgca
     8521 gccaccccgg ggcaggccta cgcctgcacg gtgcggcgga attctcccgg cgacgcgccg
     8581 gtccatttcc tgaacgcgcg atgaaacgca ctgcgctccg agaaccccaa ttccagggcg
     8641 atgtccatga cgctcctggt cgtatggctc aagtagccga tggcaagatc gcgccgcagc
     8701 tggtccttga tcgactgata agactcgcct tcctcgtgca aacggcggcg catggttgcc
     8761 gcggtcatat tcagatccga ggccagctgc tcgaaatccg gaacttcgcc aggcaaaaac
     8821 tggcgcagcc gccggcgaac tcgcgccgtg aggctgctgc cgttcttgta cttcaatagg
     8881 atactctccg gtgcggtacg cagaaactcc ttgagcgagc gctcgtcctg caccaccggc
     8941 agatccagat aactcgagtc aaaggcgagc agcgtgttcg gcttgtcgaa gctcaagtcg
     9001 gcacagtaca tgagacgata ctcggcgctg tggccgggtt cggcgtagct gaattcggtg
     9061 cgcaggatcg ggatgcggcg gccgatcagc cagcaggaga caccgtacag cagcatcaac
     9121 aacagctcgt gggcgaaaat gcgttggctc gcgccggccg cccgctcacg caaaactatt
     9181 cttgcttcct tggagttgcg ctccaaggtg ccggagatgt cgtccaagat cagcgcgtag
     9241 aaacgcaaac tgcgctccag cgcctggccc aaggtcttgc aattgagcac cgagtggcaa
     9301 agcatcgcga agctcccggc cttcatgcgg cgcgagtctt ggccgaagaa ttcgtcatcg
     9361 agcgcgagcg caatactgcg ccacagggcg ccatagtgct ttgcggagac tcgcgcctgg
     9421 ggtacttgca gcagactcga ggatagcccg acattcgcca gcagctcgtc cgcgttcaag
     9481 tcccgggcgc ggacgctttg cagcgcggcg gcgacgaaac agatggcgat ggttccgggc
     9541 tccggcgacc gtgtagcagt gcccgccgtc ccccgggtcg gggacaactc cgtgttgccg
     9601 ttccgctgac gcattttgcc gtcagtttac gcgatacgcc ccccgtctgc cctgcccctg
     9661 gggggttcgt caggctaaac taccccgtcg cgcaacgacc caaggaacac gctcatgaca
     9721 attcaacccg ccacactcgt aaagccccca gtcactgtca aattgctcct cgacggcaag
     9781 ttcgtcgagt cgcgaagcgc cgagtggcac gacgtcgtca acccggcgac ccaggaggtg
     9841 cttgcgcaag ttccgttcgc caccgcagag gagatcgagg cggcgatcaa ctcggccaaa
     9901 catgcctaca agacctggaa gaacactccg ctcgccacgc gtgcgcgcat catgttgaag
     9961 ctgcaagcgc tggtacgcga ccacatgagc cgcatcgcgc agaccttgag cgccgagcaa
    10021 ggcaagacgc tggccgatgc cgaaggcgat gtgttccgcg ggctcgaggt agtggaacac
    10081 gcctgctcga tcggcagcct gcaactgggt gaattcggcg agaacgttgc cggcggcgtc
    10141 gacacctaca ccctgcggca gccgatcggc gtgtgcgccg gtatctcgcc cttcaatttt
    10201 cccgccatga tcccgctgtg gatgttcccg atggccattg tctgcggcaa cacgttcgtg
    10261 ctcaaaccct cggagcagga cccgctcacg cccatgctgt tggcggaact cgccctcgaa
    10321 gccggcgtgc cgccgggcgt tctcaacatc gttcatggcg gcaagcgcgc cgtcgacgcg
    10381 ctgtgcactc acccggacat tgccgcggtg tccttcgtcg gctcaaccgc ggtcggtacg
    10441 cacgtctaca atctggcttc gcaaagcggc aagcgcgtgc aatgcatgat gggcgcgaag
    10501 aaccatgcgg tcgtgatgcc ggacgccaac aaggaacaat cgctcaattc attggtcggc
    10561 gcgactttcg gcgccgccgg acaacgctgc atggcaactt cggtcaccgt gttggtgggt
    10621 gaagcgcgca agtggatacc ggacatcgtc gccaaggcaa agacactcaa ggtcaatgcc
    10681 ggcgtggaga aaggcgccga tttgggaccc gtggtgtcga agaacgccaa gcagcgcatc
    10741 ctcggtctgc tggaggacgg gatcaaggaa ggcgccaagc tcgagttgga cggccgctcc
    10801 atcaaagtgc cgggctacga gaaaggcaac ttcatcgggc cgaccatttt ctcggcggtg
    10861 acgcccgcga tgaagatcta caccaccgag attttcggcc cggtgatgat catcgtgagt
    10921 gtcgacacct tggacgaagc gatttcactc atcaacaata atcccttcgg caacggcacc
    10981 ggaatattca cccaaagcgg cgcggtagcc cgcaagtttc aaagcgaaat cgatgtgggc
    11041 caggtcggga tcaatgtacc gatcccggtg cccgtgccgg tattcagctt taccggttcg
    11101 cgcggctcca agctcggcga tctcggcccg tacggcaaac aggcggtgca gttctatacg
    11161 caaaccaaga ccgtgacgtc gcgctggttc gacgacagca gcacgcccgg cgcggtcaac
    11221 accaccatca gcctaaaatg aaacctccgc atggaagttt tgagtgaaat tcgcaatcac
    11281 gttgccttca ttacgctgaa tcgcccggca gcgctcaacg cgttgtcgct cgagatgttg
    11341 ctgcgcctgc gcacgctcct gagcgaatcc gcgtcggacc cgcaagtgca tgccgtcgtg
    11401 ttgcaaggtg cgggcgatcg cgcattttgc gccggcggag atatccgcgc cctgtatcaa
    11461 agcttcaaga gcacggggac tttgcaccgt caattttttt tgacggaata tccgctcgac
    11521 tacgcgcttt acgcctatcc caagccctac gtcgtcgtga tggacggcat caccatgggc
    11581 ggaggcatgg ggatcgctca aggctcggcc atgcgcgtgg tgggcgagcg aacccgcatt
    11641 gcgatgccgg aagtggcgat cggctttttc ccggacgtgg gcgccagcta tttcttgtcg
    11701 cgtcttccgg gagcgctcgg aatctacctg gcgttgaccg gggtgcagat ccgcggcgcc
    11761 gatgcgcttt attgccactt ggccgacgtg tacctgacgc cgcaggcgat cgcagccttg
    11821 atggacgacc tgtcgaccct cgattggagc gatgactgcg ccaacgactt gcggcgattc
    11881 atccatgcgc gagcggcgca aggtttggcg gcaccgtcgc tcagccgctt gcgcacgggc
    11941 atcgatgcgc atttcgtgca cccgaccgtc cgtgccatac tcgcctctct cgatgcggaa
    12001 acgaatgccg aatacaccga ctgggcgcga cagacggcag cgctgatgcg cagccgctcg
    12061 ccgaccatgc tgtgcgtcac cttacgtcaa ttgcagcgcg gcaagacgat gaccctcgcc
    12121 gcctgtttcc gcatggagtt gtcgatggtg cagcaatgtt tcgagcaggg agatttcatc
    12181 gaaggggtgc gtgcgctgct cattgaccgg gacaatgcgc cgcactggac accgaatcgg
    12241 gtcgaagacg tcacggacgg catggtcgac acgttctttc gcagccgctg gacagcggcc
    12301 ggacatccat tatcaatctt gcagggcttt tgaggattca ctatgcagtt aaaagacacc
    12361 cacgccgtca tcaccggcgg cgcctccgga ctgggactcg ccgtggcgaa acgagtgata
    12421 ctcgccggcg gcaaggcgac cctcctcgat gtgaatgcag cacagggcgc agcggccgcg
    12481 gcagcgctcg gcgagcgcgc cagcttcatc gcaaccgacg tgaccgacga atcggcggtc
    12541 gatggcgcca tcgatcgcgc ccgcgagcgg ctgggtgcca tccaggccgc ggtcaattgc
    12601 gccggcatcg cgactcccac ccgcctggtc ggcaaacagg gtgccatgag cggtgcatat
    12661 tttcgcaaga tgatcgaaat caacctgatt ggcaccttgt tggtcgccaa ggccgccgcc
    12721 gtggccatgc aactcaactc gccgaacgcc gagggcgaac gcggtgtcat catcatgacc
    12781 gcctcggtcg ctgccttcga cggccagatc ggtcaggtgg cctatgcagc ctcgaagggc
    12841 ggcattgtcg ggatgacgct gccgatggcg cgcgacatgg cttcctcagg cattcgcgtc
    12901 atgaccatag ctcccggatt gttcgccacg ccgatgatga agggtctccc tcaggaggca
    12961 caggattcgc tcggtaagca aacgccgttt ccaccccggc tcggcgagcc cgatgagtac
    13021 gccgccttgg tcggacacat catcgagaac gtcatgctgg acggcgagac catccggctc
    13081 gatggcgcaa tccgaatggc gccaagatag cggcattgtt gcgctagcga cccggcacct
    13141 gacctacgag ttccttgcgg cgcgcgacgt cgcgcaagat ttcgcgctgc cgcgcttcgc
    13201 cggcagtcga ccattcggct atttcgttca aggtacggcg gcatcccacg cacaacccct
    13261 gcgtatccaa tcgacacaca tcgatgcacg gcgatggcac ggcggcggcc atcagcgatg
    13321 cgccgcccat tctcgtaaca gccgatgaat ggcatcgagt ccgtcggtga gcgcggccgg
    13381 ccccggttgc aagatgaggg gcgacttgat ctcgtgaagc tcgccgtcgc gcaccgccgg
    13441 tacggcgttc catccgagcc gcgctgcgac ttgctcgggg cgaaagcgtt taccgcacca
    13501 ggatccgatg atgatgtcgg gcgcgcggcg gatcacctcg ttggcgtcgc tcaaaattcg
    13561 atccttgccg agtgaacaca ccgcgcgctc gggaaacacg tcatcgccgc cggcaatgcc
    13621 gatcagttcg ctgacccagc ggatgcctgt gatgagcggt tgatcccatt cttcgaaata
    13681 cactcgcgga cggcggctca tcccggcggc
//
LOCUS       DASWZT010000232         4250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_25980, whole genome shotgun sequence.
ACCESSION   DASWZT010000232 DASWZT010000000
VERSION     DASWZT010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25980"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VGE92_06435"
     CDS             complement(<1..185)
                     /locus_tag="VGE92_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY1313492.1"
                     /translation="MSEPALAADERAARIDLAAAFRWFARLNMHESVANHLSVAVSAD
                     GSRFLMNPRGRHFSRICA"
     gene            complement(182..1840)
                     /locus_tag="VGE92_06440"
     CDS             complement(182..1840)
                     /locus_tag="VGE92_06440"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF002550.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEY1313493.1"
                     /translation="MRSAAYDYIIVGAGSAGCVLAARLTEDAGTRVLLLEAGPPDRSW
                     RIHMPAAVGSLLSSERFNWNYISDPEPHLNGRRLTHPRGRVLGGSSSINGMVYIRGHA
                     LDYNAWESSGLEGWGYADVLPYFKRAERHMLGGDAYHGHLGPLRVEAPNIAQSPLASS
                     FLAAARDAGYPVSADPNGERQEGFGRVDRTTYAGRRCSAARAYLSPARARANLSIATG
                     ALVQCVLFSGTRAIGVQYSLGGRTLETHAAEVILSAGAINSPQLLQLSGIGAADHLTR
                     LGIAPRCDLPAVGAHLNDHPDIVVQHACSQPVSIFGANRGLPKLLTGIRWFLGRGGLA
                     GSNHFEAGGFIRSRAGVEHPDLQLTFMPLAIKPGTVEDVGMHSFQVHIDLMRPKSLGS
                     VAVRSADPRVAPVICFNYLAAPEDRQVLRAAVRLTREILAQPSLAAYRGPELNPGESV
                     QSDAQIDAWVRGAVETCYHPVGTCRMGAVERGAVVDGDCRVYGVTGLRVVDASVMPAI
                     VSGNTNAPTIMIAEKMSDRIRGRAALMRESPPVWIHPHYATAQR"
     gene            complement(1840..2970)
                     /locus_tag="VGE92_06445"
     CDS             complement(1840..2970)
                     /locus_tag="VGE92_06445"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY1313494.1"
                     /translation="MDAALIWSEEDQRAGYTLPPAFFYDPQVFEREKTSIFFKSWHLV
                     GHLSELQEPGSFVTHDFLDQSILVVAGRDGVIRAFHNVCQHRGNRLVDKRRGHVALLT
                     CAYHAWTYSLDGCLRAASHGGETPGFDKAAHGLKAVQVAVFASFVFVNMDASAPSIPA
                     MAPGAEEQIRRFIPDLDRLTLLEEIDVPVAANWKVIQENSIEGYHFDQSGPAHKQLVQ
                     LIDFAGYRLTPHDRWWSYIGPPKRGTTTAYGVALEGATWQSDWFFNFGLWPNATLYVF
                     PYADVVGTFIMNPTGPETSSLRFGYYGVKGRPLPEATRACIRWMNQDLGPEDIRLNVS
                     NQKGLKSLGFSRGRYIIGEDFANRSEHLVRHFHQLCYDAIRS"
     gene            3071..4069
                     /locus_tag="VGE92_06450"
     CDS             3071..4069
                     /locus_tag="VGE92_06450"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HEY1313495.1"
                     /translation="MQRIAFLLLPEFSSLGLAAAMEPLFIANWLAQRPVFTWSTLSVD
                     GKAVRASNGSVTAVNADLQALDFGSAFVLASFDPAAAARQPEALRWLKRMARSGAELG
                     GIENGSWLLARAGLLKGHEVAVHWDNLEGFQEAFPACRASAQLYCRSANRFSCAGASA
                     ILDLMIAFIGWHGEADLAAEVAEHLLLARVRAPNAAQRHGADTAEEGADALVAKATQL
                     MAAHLEEPLSCRELARGVGLSLRQLERRFAQELSSTVLQEYRRIRVAKAHQLLQQTTL
                     TVIEVAIACGFSSPEYFSRVYRLQFGCLPSRDRKQSITAPVLRHPRKRRGLKAAHH"
BASE COUNT          833 a         1295 c         1368 g          754 t
ORIGIN      
        1 gcgcagattc gagagaaatg gcggccgcga ggattcatga ggaagcgcga gccatcggcc
       61 gacaccgcga cgctcaagtg atttgccacc gattcgtgca tattgagccg tgcgaaccaa
      121 cgaaacgcgg cggcgaggtc gatgcgggcg gcgcgttcat ccgcagcgag tgcgggttcg
      181 ctcatcgctg cgccgtcgca tagtgaggat ggatccacac aggaggcgat tcgcgcatga
      241 gggccgccct gccacggatc cgatcgctca tcttctcggc aatcatgatg gtgggcgcat
      301 tggtgttgcc gctcacgatc gccggcatca cggaggcgtc cacgacccga agcccggtca
      361 cgccgtagac gcggcaatca ccgtcaacga cggcaccgcg ctcaaccgcg cccatgcggc
      421 acgtgcccac ggggtgataa cacgtctcga cggcaccgcg tacccaggcg tcgatctgcg
      481 cgtcgctctg cacggattcg ccggggttca actccgggcc tcgataggcg gcaaggcttg
      541 gttgtgcgag gatttcgcgc gtcaggcgca ctgctgcgcg cagcacttgg cgatcctcgg
      601 gtgcggcgag atagttgaaa caaattacag gcgcgacgcg cggatcggcg cttcgaaccg
      661 cgaccgagcc caagctcttc ggccgcatga gatcaatgtg cacttggaag gaatgcatgc
      721 ccacatcctc caccgtgcct ggcttgatcg ccagcggcat gaaggtcaat tgcagatcag
      781 ggtgttcgac gcccgctcgg ctgcggatga agccgcctgc ctcgaagtga ttcgagccgg
      841 ccaagccgcc ccgacccaag aaccagcgga ttcccgtgag cagtttcggc aggccgcgat
      901 tggcaccaaa gatcgaaacc ggctgcgagc aggcgtgctg cacgacgatg tccgggtgat
      961 cgttcaaatg ggcacccacg gcgggtaaat cgcaacgcgg cgcaatgccc aatcgggtca
     1021 gatggtcggc ggcaccgatc cccgagagtt gcaaaagttg cggcgagttg attgcgcccg
     1081 ctgacaaaat gacctcggcg gcgtgagttt cgagcgtccg gccgcccagg ctgtattgaa
     1141 cgccgatggc gcgcgtgccg ctgaataaca cgcactgtac cagcgcgccg gtcgcaatgc
     1201 tgagattcgc acgggcccgt gccggcgata aataggcgcg cgcggcgctg caacggcgac
     1261 ccgcataggt ggtgcgatcg acgcgcccga agccttcttg acgctcgccg ttcggatcag
     1321 cggagaccgg ataccccgca tcgcgagccg cggcgagaaa gctcgatgcc aatggggatt
     1381 gtgcgatgtt cggcgcctcg acccgcagcg gacccaagtg gccgtggtag gcatcgccgc
     1441 cgagcatatg ccgttcggca cgcttgaagt agggaagaac atcggcgtag ccccatccct
     1501 cgagtccgct cgactcccag gcgttgtagt cgagcgcgtg gccgcggatg tacaccatgc
     1561 cattgatgga ggaggagccg ccgagcacgc gcccgcgcgg atgcgtgaga cgtctgccgt
     1621 taagatgcgg ctcaggatcg gatatatagt tccagttgaa gcgctccgag gagagcagac
     1681 tgccgaccgc tgcgggcatg tgaatccgcc agctgcgatc gggcggcccc gcctcgagaa
     1741 gcagtacgcg agtccccgca tcctcggtga gccgcgccgc gagcacacat cccgccgatc
     1801 ccgcgccaac gatgatgtag tcgtacgcgg cgctgcgcat cacgagcgaa tggcgtcgta
     1861 gcagagttga tggaagtggc gcaccaggtg ttcactgcgg ttggcaaaat cctcgccgat
     1921 aatgtagcgg ccgcggctga accccaaaga cttcaagcct ttttgattgg acacattcag
     1981 gcgaatgtcc tcgggcccca agtcctggtt catccaccgg atgcacgccc gggtggcttc
     2041 cggcagcggg cgacccttca cgccgtagta gccgaagcgc agcgaggagg tctcgggtcc
     2101 ggtcggattc atgatgaaag tcccgaccac gtctgcgtac ggaaagacgt acagggtggc
     2161 gttcggccat agaccgaaat tgaaaaacca gtccgattgc caggttgcac cctcgagcgc
     2221 aacgccgtac gccgtggtcg tgccgcgctt cggcggcccg atatacgacc accaacggtc
     2281 atgcggcgtc agcctgtagc ccgcgaaatc gatgagttgc accaattgct tgtgcgccgg
     2341 gccggactgg tcaaagtgat agccctcgat ggaattctct tggatcactt tccagttggc
     2401 cgccaccgga acatcgatct cttcgagcag cgtcaaccga tcgagatcgg ggatgaagcg
     2461 gcgaatctgc tcctcagcgc cgggcgccat tgcggggatg gagggtgccg atgcgtccat
     2521 gttcacgaac acgaatgagg cgaacaccgc gacttgcacc gctttcaagc catgcgcggc
     2581 cttgtcgaat cccggcgtct cgccgccgtg ggaggcagcc cgcaggcatc cgtccaggct
     2641 gtacgtccag gcgtgatagg cgcaggtcaa aagcgctaca tggccccgcc gcttatcgac
     2701 caagcggttg ccgcgatgct ggcagacatt gtgaaaagct cggattacgc cgtcgcgacc
     2761 cgcgacgacg agaatgcttt gatcgagaaa atcatgagtg acaaagctac ccggctcttg
     2821 gagttcgctc aggtgcccca ccaagtgcca ggacttgaaa aaaatcgagg tcttctcgcg
     2881 ttcgaatacc tgcgggtcgt agaagaacgc cggcggcaga gtatacccag ccctttgatc
     2941 ctcctcgctc caaatgagtg ctgcgtccat gagagtcctc gctgacaaag ccgcaacgct
     3001 acggcggatc ggctggcgaa acctgctaaa aagcgtcatg atgatgtcgc attcttgagt
     3061 tagggccggc atgcaaagaa tcgccttttt acttctgccg gaattctcgt cgttgggact
     3121 cgcggcggcc atggagccgc tgttcatcgc caactggctg gcgcagcggc ccgtgttcac
     3181 ctggtcgacg ctctcggttg acggcaaagc ggtgcgtgcc agcaacggca gcgtgaccgc
     3241 ggtgaatgcg gacttgcagg cgctcgattt cggcagcgca tttgtcttgg cgagtttcga
     3301 tccggcggcg gcggcccgcc agcccgaggc gctgcgttgg ctcaagcgca tggcacggtc
     3361 gggggcggaa ctcggaggta tcgaaaacgg cagctggctg ttggcgcgcg ccggtcttct
     3421 caaaggccat gaggtcgccg tgcattggga caatctcgag ggcttccaag aggcattccc
     3481 ggcatgccgc gccagcgcgc agctgtactg ccgcagcgcc aatcgcttct cgtgtgccgg
     3541 agcatccgca attctcgacc tgatgattgc ctttatcggc tggcacggcg aggcggatct
     3601 ggcggccgaa gtcgctgaac atttgttgct cgcgcgtgtg cgtgcgccaa acgccgcaca
     3661 gcggcacggc gccgacacag ccgaggaggg cgccgatgcg ctcgttgcca aggccacgca
     3721 gctgatggcg gcgcacctgg aggaacccct gtcgtgtcgg gagctggcgc gcggcgtggg
     3781 actgtcattg cggcaactcg aacggcgttt cgcacaggaa ttgagctcga cagtattgca
     3841 ggagtatcga aggattcgcg tggccaaggc acaccagctg ttgcagcaga cgacgttgac
     3901 ggtcatcgag gtcgcgatcg cctgtgggtt ttcatcgccc gaatatttca gccgcgttta
     3961 tcggctgcag ttcggctgtc tgccgagccg cgatcggaaa caatcgatta cggcaccggt
     4021 ccttcgccac cccagaaaac gccgcgggct caaggcagct catcactagg gcgatcgtcc
     4081 gaggtttgcg aaagctccac agaaaagggc aaaatcatgg tccgaaccgc agcgaggagc
     4141 catcatgtgc gaactggacg atatggaaga atattcaaag caagccagcg gcgtttcacg
     4201 gcgtcagttc agtacattgg cgttgggcgc tggattggcg gcggctctcc
//
LOCUS       DASWZT010000233         6352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_83987, whole genome shotgun sequence.
ACCESSION   DASWZT010000233 DASWZT010000000
VERSION     DASWZT010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6352
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83987"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1257)
                     /gene="glyA"
                     /locus_tag="VGE92_06455"
     CDS             complement(4..1257)
                     /gene="glyA"
                     /locus_tag="VGE92_06455"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY1313496.1"
                     /translation="MFSSQMTIAGFDPELAKAIADERNRQEDHIELIASENYTSPRVL
                     EAQGSVLTNKYAEGYPGKRYYGGCEFVDVAESLAIERAKQLFGAAYANVQPHSGSQAN
                     AAVYLALLNPGDTILGMSLDHGGHLTHGAKVNFSGKLFNALQYGIKSGNGEIDYDQVQ
                     SLAREHRPRVVIAGFSAYSRVIDWQRFRSIADSVGAYLFVDMAHVAGLVAAGAYPNPV
                     PFADVVTTTTHKTLRGPRGGLILARPHPELHKKFNSLVFPGTQGGPLMHVIAAKAVAF
                     LEALQPEFKTYCAQVVTNARVMAQVLQERGYKIVSGGTDNHLFLLDLVDKNITGKDAD
                     AALGRAHITVNKNAVPNDPRPPAISSGLRIGTPAVTTRGFKEAEVRQLTGWIADVLDH
                     MGDESVIERVRAEVSTICRRYPVYG"
     gene            1411..3084
                     /gene="ettA"
                     /locus_tag="VGE92_06460"
     CDS             1411..3084
                     /gene="ettA"
                     /locus_tag="VGE92_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEY1313497.1"
                     /translation="MAQFIYTMNRVGKVVPPKRIILRDISLSFFPGAKIGVLGLNGSG
                     KSTLLKIMSGVDKEIEGEARAQAGIKVGYLPQEPQLDEQKTVREVVGEGVGDVQSLID
                     RFNAISDRFAEPMSDDEMNKLMEEQAKMQDKIDAVGGWELERKLEIAADSLRLPPWDA
                     VVGKLSGGEKRRVALCRLLLSKPDMLLLDEPTNHLDAESVAWLEHFLEEYPSTVIAVT
                     HDRYFLDNVAEWILELDRGHGIPWQGNYSSWLEQKEQRLAQEERQQDALAKTLKRELE
                     WVRTNPKARQAKSKARLQRYEELASQDFQKRNETNEIYIPPGDRLGDLVIETKNLRKG
                     YGERLLIDSLSFNLPPGGIVGIIGPNGAGKTTLFRMLSGAEKPDAGEIRVGPSVKLAY
                     VDQSRQSLNDKNTVWQEISGGLDLIKVGNYETSSRGYVGRFNFKGVQQQQSIGELSGG
                     ERNRVHLAKVLRAGGNVLLLDEPTNDLDIETLRALEEALLAFPGCAVVISHDRWFLDR
                     VATHILAFEGDSQVIWFEGNYQEYVEDYRKRKGEDAAQPHRIRYKPLTG"
     gene            3093..3770
                     /locus_tag="VGE92_06465"
     CDS             3093..3770
                     /locus_tag="VGE92_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313498.1"
                     /translation="MYITPQDELHVEFQGIAVTCPHCETLTHLTPVAIPKFEDLSRRK
                     PKNIGVVFRCDACAEPVFLKFAAKAYGAARVELAANFSEVERAREKFPLTYLPEEAEA
                     LFKEALNCYAAGCFNAFGAMSRRTAQSLFRELGERGKLELFDSLQEVRSLAELDDDTF
                     AALRSVLFGSDSDPWPHQPDISPERAGVLLEVMRDLLYQTFVRKARLLQAVTFRKFVA
                     PTRAEAS"
     gene            complement(3791..>6352)
                     /gene="purL"
                     /locus_tag="VGE92_06470"
                     /gene_synonym="purI"
     CDS             complement(3791..>6352)
                     /gene="purL"
                     /locus_tag="VGE92_06470"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF003672.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEY1313499.1"
                     /translation="AMLIGLGGGAASSVGSGQSSSDLDFASVQRGNAEIQRRAQEVID
                     RCWAMGEENPILLIHDVGAGGLSNALPEAIAHSQRGGRIDLRAIPSAESELSPMELWC
                     NEAQERYVLALEPKSLARFAMLCERERCPFAVVGEITGDGRLVVSDPKLGTMPVDMPI
                     SVLLGKPPRTTRDVRAIHKTVTQLSTAESSIAESLERLLNLPAIADKSFLITIGDRSV
                     GGMVSRDQMVGPWQVPVADVGVTLNSYDGHGGEAMAMGERTPVAVLNAPASGRLAVGE
                     AITNIMAADIVQLSDIRLSANWMAACGEPGEDADLYATVRAVGVDLCAALGITIPVGK
                     DSLSMRTAWHDAGGEHSVVAPISLIVSAFAPVRDVRRTLTPELDLSQPTRLLLLDLGA
                     GKNRLGGSCWAQVHAISGGEPADLDSPRLLLDAFALLRGLKDAGLLLAYHDRSDGGAL
                     QSVLEMAFASHCGLALDLGAADDPIAACFAEELGAVLQVPAARAAEALDVAARHGMAA
                     LLRDIGEPQEGDEVRLDINGATLYRASRALLRRRWSAVSFALQTLRDNPDCAREEFSR
                     LSDAADPGLHAALSYDPSEDVAAPLIARGARPAIAILREQGVNSQTEMAAVFSRAGFD
                     AYDVHMTDVLSRRVRLARFHGLVACGGFSFGDVLGAGEGWAKSILFNSVARDEFAAFF
                     ARSATFTLGVCNGCQMLSALKELIPGTAGWPRFVRNVSEQYEARLTLVRVPPSQSVLT
                     TGMAGSVLPIVVAHGEGQAQFDSGSSAASLLAQGQVTLQYTDNRERPTEQYPYNPNGS
                     ALGLAGLCSSDGRVTSIMPHPERVFRTVQNSWAPKQWSEDGGWMRLFRNARLFVE"
BASE COUNT         1233 a         2069 c         1941 g         1109 t
ORIGIN      
        1 cggctagcca tacacgggat agcgccggca gatcgtcgac acttcggccc gcacgcgctc
       61 gatcacggac tcatcgccca tatgatccag tacgtcggcg atccacccgg tcagctgccg
      121 gacttcggcc tccttgaagc cgcgggtcgt caccgcgggc gtaccgatgc gcagcccgct
      181 gctgatggcc ggcggccgag gatcgttggg caccgcgttc ttgttcacgg tgatgtgggc
      241 gcgtccgagc gcggcgtcgg cgtccttgcc cgtaatgttc ttgtcgacca agtcgagcaa
      301 gaacagatga ttgtcggtgc cgccggagac gattttgtag ccgcgttcct gcaggacttg
      361 cgccatgacg cgcgcgttcg tgacgacctg cgcgcaatag gttttgaatt ccggctgcag
      421 cgcctcgaga aacgccaccg ccttggcggc gatcacgtgc atcagcgggc cgccctgggt
      481 gccgggaaat accagcgaat tgaacttctt gtgcagttcc ggatgcggcc gcgccaggat
      541 caaaccgccg cgcggtccgc gcagcgtctt gtgcgtcgtg gtcgtcacca catcggcgaa
      601 cggcaccggg ttcggataag cacccgccgc gaccaggccg gcgacatgcg ccatatcgac
      661 gaacaaatag gcgcccaccg agtccgcgat gctgcgaaag cgctgccagt cgatgacccg
      721 cgagtacgcc gagaatccgg cgatcaccac gcgcggccga tgttcccggg ccaggctctg
      781 cacctggtcg taatcgattt cgccgttccc ggacttgatt ccatattgca gggcgttgaa
      841 cagtttgccg gagaagttga ccttcgcgcc atgcgtcaag tgaccgccat gatccaaact
      901 catgccgaga atcgtatcgc ccggattgag cagcgccaga tacacggcgg cattcgcctg
      961 actgccggaa tgcggctgca cattggcata ggcggcgccg aacagctgtt ttgcccgctc
     1021 gatcgccagg ctctccgcga catcgacgaa ttcgcagccg ccgtagtagc gcttcccggg
     1081 atagccttcc gcgtacttgt tggtgagaac cgagccttgc gcttcgagca cgcgcggact
     1141 cgtatagttc tccgaggcga tgagttcgat gtggtcttcc tggcggttgc gctcgtccgc
     1201 gatcgccttc gcgagctccg gatcgaagcc ggcaatagtc atttgagaac tgaacatggg
     1261 ctaactctcc cgacagctag ccgataattg tagccgattc atatggggtt ccctagcgac
     1321 aaaccctcat cgtgacaagt ttccgctact cgcggataat tccgggcgat ccccgctcgt
     1381 tcacgcgaag aattcaacca taggaagcaa atggctcagt tcatttacac catgaaccgg
     1441 gtgggcaaag tcgtgccgcc caagcgcatt attttgcgcg acatctcctt gagcttcttt
     1501 cccggcgcca aaatcggcgt gctgggcttg aatggctccg gcaaatccac cctgctcaag
     1561 atcatgtccg gcgtcgacaa ggagatcgag ggcgaagcgc gcgcgcaggc cggcatcaaa
     1621 gtcggctacc tgccgcagga accgcagctc gatgagcaga agacggtccg cgaagtggtc
     1681 ggcgaggggg tcggcgacgt gcagagcctg atcgatcgct tcaatgcgat cagcgatcgc
     1741 tttgccgagc ccatgtcgga tgacgaaatg aacaaactca tggaagaaca agccaagatg
     1801 caggacaaga tcgatgcggt cggcggctgg gagctcgagc gcaagctgga aattgccgcc
     1861 gactcgctcc gtttgccgcc gtgggacgcg gtggtgggca agctgtcggg cggcgagaag
     1921 cgccgcgtcg cgctgtgccg cctgctgttg tcgaagcccg acatgctgct gctcgacgaa
     1981 ccgaccaacc acctcgatgc ggaatcggtg gcctggctcg aacactttct cgaggaatac
     2041 ccaagcaccg tgatcgccgt gacccacgat cgctactttc tcgacaacgt ggcggaatgg
     2101 attttggaac tcgaccgcgg gcacggaatt ccgtggcagg gcaactactc ctcctggctc
     2161 gagcagaagg aacagcggtt ggcgcaggaa gaacgccagc aggacgctct cgccaagacc
     2221 ttgaagcgcg aattggagtg ggtacgcacc aatcccaaag cgcgtcaggc taaatccaag
     2281 gcgcgtctgc agcgctacga agaactggct tcgcaggatt tccagaagcg caacgaaacc
     2341 aatgaaattt atattccccc cggtgatcgc ctcggggacc tggtcatcga aaccaagaac
     2401 ctgcgcaagg gttacggcga gcgtttgctg atcgattcct tgagcttcaa cctgccgccc
     2461 ggcggcatcg tcggcatcat cgggccgaac ggcgcgggca agaccacgct gtttcgcatg
     2521 ctctcgggcg ccgagaaacc cgacgccggc gaaatccgcg tcggcccgag cgtcaagctc
     2581 gcctatgtcg accaatcgcg ccaatcgctc aacgacaaga acaccgtctg gcaggagatt
     2641 tccggcggcc tcgatctcat caaggtcggc aattacgaga cttcgtcgcg cggctacgtc
     2701 ggccgtttca acttcaaggg cgtgcagcag cagcagtcga tcggcgaatt gtcaggcggc
     2761 gagcgcaacc gcgtacatct ggccaaagtg ctgcgcgcgg gcggcaatgt gctgctgctc
     2821 gacgaaccga ccaacgacct cgacatcgaa acattgcggg ctctcgagga ggcgctgctc
     2881 gccttcccgg gttgcgcggt cgtcatctcg cacgatcgtt ggtttctcga tcgcgtcgca
     2941 acgcatatac tggccttcga gggcgactcc caagttatct ggttcgaggg caactatcag
     3001 gagtatgtcg aggactacag gaaacgcaaa ggcgaagatg ccgctcagcc ccaccgaatt
     3061 cgctacaaac cactgacagg ttgagacgat ccatgtacat cacgccgcaa gatgaattgc
     3121 atgtcgagtt tcaggggatt gccgtgactt gcccgcactg cgagacgctg acccatctca
     3181 cgccggtggc catccccaag ttcgaagatc tcagccgccg caagccgaag aacatcggcg
     3241 tcgtgttccg ctgcgatgcc tgcgcggagc cggtgtttct caagttcgcc gccaaggcct
     3301 acggcgccgc gcgggtcgaa ctcgcggcaa acttttcgga agtggaacgc gcgcgcgaga
     3361 agtttccgct gacctatctt ccggaggaag ccgaggccct gttcaaagaa gcgctcaatt
     3421 gctacgcggc gggctgcttc aacgccttcg gcgccatgag ccggcgtacc gcacaatcct
     3481 tgttccgcga actcggcgag cgcggcaagc tcgagctgtt cgattctctg caggaggttc
     3541 gcagcctggc ggagctcgac gatgacacct tcgcagcgtt gcgctcggtg ctgttcggca
     3601 gcgacagcga cccgtggccg catcagcccg acatcagccc ggaacgcgcc ggcgtgctgc
     3661 tcgaagtgat gcgcgatttg ttgtatcaga cctttgttcg caaggcacgg ttgctgcaag
     3721 cggtgacatt tcgcaagttc gtggcaccga cgcgcgccga agccagctag cggctgatgc
     3781 gcgccgccgc ctactccacg aacaggcggg cattgcgaaa taagcgcatc cagccaccgt
     3841 cctccgacca ctgtttcggc gcccaggagt tctgtactgt gcgaaacacc cgttcgggat
     3901 gcggcatgat cgatgtcacg cgcccgtcgc tgctgcacag tccggcaaga cccaaggccg
     3961 aaccgttcgg attgtacgga tactgctcgg tcggacgttc tcgattgtcg gtgtattgca
     4021 gcgtgacctg accctgcgcg agcaggctcg ccgcactcga tccggaatcg aactgcgcct
     4081 ggccttcgcc gtgcgccacg acgatcggca gcaccgatcc cgccatgccg gtcgtcaaca
     4141 ccgactgcga cggcggcaca cgcaccaggg tcaggcgtgc ctcatactgc tcggaaacgt
     4201 tgcgcacgaa gcgcggccag cccgcggttc cggggatcag ttccttgagc gcggataaca
     4261 tctggcagcc gttgcacacg ccgagcgtga aggtggcact gcgcgcaaaa aaggccgcga
     4321 actcgtcgcg cgccacggaa ttgaaaagga tcgacttcgc ccacccctcg ccggctccga
     4381 ggacgtcgcc gaaggagaat ccgccgcagg cgaccaggcc gtgaaaccgc gccaaacgca
     4441 cccggcggct caagacatcg gtcatatgca cgtcataggc gtcgaaaccg gcgcggctga
     4501 ataccgcggc catttctgtc tggctgttga cgccttgttc acgcaggatg gcgatcgcgg
     4561 gacgcgcgcc acgcgcgatc aggggcgcgg cgacgtcctc gctcggatcg taactcaagg
     4621 cggcgtgcag gccgggatcc gccgcatcgc tcaagcgcga aaattcttca cgcgcgcaat
     4681 ccggattgtc gcgcagtgtc tgcagagcga acgataccgc gctccaacgg cgccgcaaca
     4741 gcgcgcgcga ggcgcggtac agggtcgcgc cattgatatc gagacgcacc tcatcgccct
     4801 cttggggctc gccgatatcg cgcagcagcg ccgccatgcc atgacgagcc gcgacgtcga
     4861 gcgcctccgc ggcccgagcc gccggtactt gcagcaccgc gcccaattct tcggcgaagc
     4921 atgcggcgat gggatcgtcc gccgcgccca gatcgagcgc aagtccgcaa tggctggcga
     4981 acgccatctc gagcacgctt tgcagcgcac cgccgtccga gcggtcgtga taggccagca
     5041 gcaagcctgc atccttgagc ccgcgcagca gcgcaaacgc atcgagcaat aggcgcggac
     5101 tatcgagatc ggccggctcg ccgccgctta tggcgtggac ttgggcccaa catgagccgc
     5161 ccaggcggtt cttgccggcg cccaagtcga gcagcagcag gcgggtcggc tgcgataagt
     5221 ccaattccgg cgtcagcgtg cggcgcacgt cgcgtaccgg cgcaaacgcc gagacgatca
     5281 acgagatggg cgcaaccacc gaatgctcgc cgccggcgtc gtgccatgcg gtgcgcatcg
     5341 acaacgagtc cttgccgacg gggatggtga tccccaacgc ggcgcacagg tcgacgccga
     5401 ccgcgcgcac ggtcgcgtat aaatcggcat cctcacccgg ttcaccgcag gccgccatcc
     5461 agtttgccga cagccggata tcgctgagct gcacgatgtc cgcggccatg atgttggtga
     5521 tcgcctcgcc cacggcgaga cgtcccgatg ctggtgcatt caataccgcg accggcgtgc
     5581 gttcgcccat cgccatcgct tcaccgccgt ggccatcgta gctattcaag gtgacaccga
     5641 catcggcgac cggcacttgc cagggtccca ccatctgatc gcggctcacc atgccgccga
     5701 cgctgcggtc gccgatggtg atgagaaacg acttatccgc aatcgccggc aaattgagca
     5761 accgctcgag cgactcagca atgctcgact cggcggtcga caattgcgtc acggtcttgt
     5821 gaatcgcgcg cacatcccgg gtcgtgcgcg gcggcttgcc cagcaacacg ctgatgggca
     5881 tgtcgaccgg catcgtgccg agcttgggat cgctgaccac caagcgccca tcaccggtga
     5941 tctcgcccac caccgcgaag ggacagcgct cgcgctcgca cagcatcgcg aagcgcgcca
     6001 ggctctttgg ttcaagcgcg agcacatagc gctcctgcgc ttcgttgcac cacagttcca
     6061 tcggcgacaa ttcagactcg gcacttggaa tggcgcgcag gtcgatgcgc ccgccgcgct
     6121 ggctgtgcgc gatggcctcg ggcaatgcat tggatagtcc gcccgcgccg acatcgtgaa
     6181 tcaacaggat cggattctcc tcgcccatcg cccagcagcg atcgatcact tcctgagcac
     6241 ggcgttgaat ctcggcgttg ccgcgctgca cggaggcgaa atccaggtcg gaggaactct
     6301 ggccgctgcc gaccgaagaa gccgcccctc cgcccaaccc gatgagcatc gc
//
LOCUS       DASWZT010000234         3910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_55095, whole genome shotgun sequence.
ACCESSION   DASWZT010000234 DASWZT010000000
VERSION     DASWZT010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55095"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1537)
                     /locus_tag="VGE92_06475"
     CDS             complement(<1..1537)
                     /locus_tag="VGE92_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEY1313500.1"
                     /translation="MSDKAVKVAGEHGTTIERRAVLRGAAALLGTASASILGSAAASA
                     DPPSSLGKPLVIAQAGTAIVQTQSGQVAGYVHAGIFAFKGVPYADTPAGANRFLPPLR
                     CKPWTGVRSSRQYGYVAPQGARSGWANDEEAFMFAWDDGVQNEDCLRVNLWTPGINDG
                     RKRPVMVWLHGGGFAAGSGQELRSYDGENLARRGDIVLVSLNHRLNAFGYLDLSKYGD
                     KYASSANVGMLDIVAALEWVRDNIAAFGGDPKSVTLFGQSGGGGKVGALMGMPAAQGL
                     FHRAIIESGSMLRVATPEKAQTLAAAIVGELGLNRSSIDQIHALPYTQILAASDKVLR
                     ERNPPLPGGVPNFRRMAETLGFAPVLDGKIIPAHPFDPKASAMSADVPMIIGTTLNEF
                     VTAINHPEYELMTAAEVERRVKDLFGDKSAAILTAFRSRTPEAKPFDLWSRIAASTVR
                     ENAIKQAAAKAALGKAPAYLYWFTWQTPILNGRPRAFHCSEIAFVFDNTDRCDTMTGG
                     VPEA"
     gene            1662..2903
                     /locus_tag="VGE92_06480"
     CDS             1662..2903
                     /locus_tag="VGE92_06480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="parallel beta-helix domain-containing protein"
                     /protein_id="HEY1313501.1"
                     /translation="MRHISVAFLCLIAAACGQQANRSPPQSLTAEDADFQKHLQEQFL
                     DAKPGTVIVIPPGKHALDRVLTLRASGVTIRGAGVDKSILSFKNEISGPEGILVYGND
                     FAIENLSIEDTKGDGLKINDGENITIRGVRVAWTGGSQTSNGAYGIYPVKSRNILIED
                     SSSWGASDAGIYVGQSTNVVVRRCHAEQNVAGIEIENTVNADVYGNVATGNTGGILVF
                     NMPNLSQAGHSTRVYQNKVYKNNVANFAAKGAAVASVPAGAGVVVNSNSKVEIFDNDI
                     ADNQTANLIISAYFSTNYYNKPGIDPNYDPYPRSIFVYDNRFSGGGDSPDGLDLKTLK
                     VAMFGLNGHFPDILWDGYRDEKNLVNGQPPADERICVKNAGAAVLNADGPHKYKNPTT
                     ETALFQCELPKLPPVELPSHT"
     gene            2914..>3910
                     /locus_tag="VGE92_06485"
     CDS             2914..>3910
                     /locus_tag="VGE92_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SO2930 family diheme c-type cytochrome"
                     /protein_id="HEY1313502.1"
                     /translation="MSLKQALSRVALVAGVFSGSWLAGCSHPHPAVHYFAHGRPQHLS
                     EWQVVYSEGGRLEPNERVVTYDLNTPLFSDYAHKFRTVWMPAGKAARYAVADTFDFPV
                     GTIISKTFYYPRAAGAERAVLRTYDQSRDFAGPGLDLANVRLIETRILVRREQGWEAL
                     PYVWNADQTDAELARTGDEVSLELVASGEANEPFTYVVPNEVQCAGCHVADLKAKSIA
                     PIGLKARHLNRLYTYATGAENQLSYLQKVGYLAGLPALADVPRNANWRDAAQPLDARA
                     RAYLDINCAHCHSATGPADTTGLTFESITATDRHLGICKPPVAAGRGTGDHLYDIV"
BASE COUNT          762 a         1230 c         1198 g          720 t
ORIGIN      
        1 gcgcctccgg aacgccccct gtcatcgtgt cgcaacgatc ggtattgtcg aacacaaatg
       61 caatctccga gcagtggaaa gcgcgcggcc ggccattgag gatgggcgtt tgccacgtaa
      121 accagtacag atacgcgggg gctttgccga gtgcggcctt cgcggccgct tgtttgatgg
      181 cgttctcgcg caccgtcgag gcggcgatgc gcgaccacag gtcgaacggc ttcgcctctg
      241 gggtgcgaga gcgaaaggcc gtgagaatcg ccgcggactt gtcaccgaaa agatccttga
      301 cccgccgctc gacttccgca gccgtcatga gctcatattc cgggtgattg atcgcggtca
      361 cgaattcgtt gagcgtcgtg ccgatgatca tcggaacatc ggccgacatc gcggaagcct
      421 ttggatcgaa gggatgcgcg gggatgattt tcccgtcgag aaccggcgcg aatcccaacg
      481 tttccgccat gcggcgaaaa ttaggcacgc cgcccggaag cggcggatta cgttcgcgca
      541 ggaccttgtc gctggcggcg agaatttgcg tgtacggcag tgcgtggatt tggtcgatgc
      601 tcgagcggtt caggcccagc tcgccgacga tggctgcggc cagcgtttgc gccttctcgg
      661 gcgtggccac gcgcagcatc gatccgcttt cgatgatcgc gcggtgaaat agtccttgag
      721 cggccggcat gcccatcaga gcgccgacct tgccgccgcc gccggactga ccgaacaggg
      781 tgaccgactt tggatcgccg ccgaaggccg caatattatc gcgcacccac tcgagcgccg
      841 cgacgatgtc gagcatcccg acattggctg agcttgcgta tttgtcgccg tacttcgaca
      901 gatcgagata accaaacgca ttcagccggt gattcaagga tacgagcacg atgtcgccgc
      961 gccgggcgag gttctcgcca tcgtaggagc gcaattcctg accggagccc gcagcgaagc
     1021 cgccgccgtg caaccacacc atgaccgggc gttttcgccc atcgttgatg cccggcgtcc
     1081 acaggttgac gcgcaggcag tcttcgtttt gaacgccgtc atcccaggca aacatgaagg
     1141 cttcttcgtc attcgcccag ccgctgcgcg cgccctgcgg cgcaacgtaa ccgtattgac
     1201 gggagctgcg tacccccgtc cacggcttgc atctgagcgg cggcaagaat cgattggcgc
     1261 ctgcgggcgt atctgcatag ggcacgcctt tgaaggcaaa aatccctgcg tgcacataac
     1321 cggcgacttg tccggattgg gtttgcacga tggccgtccc cgcttgcgcg atgaccagcg
     1381 gtttgccgag actcgacggc ggatccgcgc tggccgcggc cgagcccagg atcgatgcgc
     1441 tggcggtacc gagaagcgcc gctgcgccgc gtagaaccgc gcgacgctct atggtcgtgc
     1501 cgtgctcacc cgccaccttc accgctttat cactcacgtt gtcccccttg cagcgccacg
     1561 ccagccgctt attgagccag catgttacac tgctgtacgg gatgcgaggc gtcgcgacgg
     1621 cgcccgcatc cgggtccgaa taacaactga ttggggaaat catgcgacac atcagcgtcg
     1681 cgtttttatg tcttatcgcc gccgcctgcg gacaacaggc caaccgatcg ccgccgcaga
     1741 gcctcactgc cgaagacgcg gatttccaga agcacctgca ggagcagttt ctggacgcca
     1801 agcccggcac ggtgatcgta attccgcccg gcaagcacgc tctcgatcgc gtactgacgt
     1861 tgcgcgccag tggcgtgacc atccgcggcg ctggcgtcga caagagcatc ctgtcgttca
     1921 agaacgagat ctcgggcccg gaagggatct tggtgtatgg caacgacttt gccattgaga
     1981 atctctcgat cgaggacacg aagggcgatg ggctcaagat caatgatggc gagaacatca
     2041 cgatccgcgg cgtccgcgtc gcctggaccg gagggtcgca aacgagcaac ggcgcttacg
     2101 gcatctaccc cgtcaagtca cggaatatct tgatcgagga ttcttcctcc tggggtgcat
     2161 cggacgccgg catctatgtc ggtcagtcca ccaacgtggt cgtgcgccgc tgccacgcgg
     2221 agcaaaacgt cgccggcatc gagatcgaaa acacggtcaa tgccgatgtt tacggcaacg
     2281 tcgcgaccgg aaacaccggc ggcattctcg tgttcaacat gccaaacttg agccaggcgg
     2341 ggcactccac gcgggtgtac caaaacaagg tgtacaagaa caacgtcgcc aacttcgctg
     2401 ccaagggtgc cgcggtggcg agcgtcccgg ccggtgcggg tgtggtcgta aattcgaata
     2461 gcaaggtcga gatcttcgac aacgacatag ccgacaatca gacggccaat ctcataatct
     2521 cggcgtactt ctctaccaac tattacaata agccgggcat cgatccgaac tacgatccgt
     2581 atccgcgatc gatttttgtc tacgacaacc gcttctccgg aggcggcgac tctcccgacg
     2641 ggctggacct caagaccctg aaggtggcga tgttcgggct caacggacat ttcccggaca
     2701 tcctctggga cgggtatcgt gacgagaaga acctcgtcaa cggccagcca cctgcggatg
     2761 agcggatctg cgtcaagaac gccggtgccg cggtgctcaa cgccgacggt ccccataaat
     2821 ataagaatcc cacgaccgag acggcgctgt ttcaatgcga actgccgaag ctgccgccgg
     2881 tggaactgcc cagtcacacg tgatatcggc gtgatgtcgc tcaagcaggc gctctcgcgg
     2941 gtcgccctgg tcgcaggggt gttcagcgga tcttggctcg cgggctgctc gcacccgcac
     3001 ccggcggtac attatttcgc gcacgggcgg ccgcaacacc tgagcgaatg gcaagtggtc
     3061 tatagcgagg gaggccggct cgaaccgaat gagcgcgtag tgacctatga cctcaacacg
     3121 ccgctgttca gcgactacgc gcacaagttt cgtaccgtat ggatgccggc cggcaaagcc
     3181 gcgcgctacg cggtggccga cactttcgat ttccccgtgg gcaccattat cagcaagacg
     3241 ttctattatc cgcgcgcggc cggcgccgag cgcgcggtgc tgcgcaccta tgatcagtcg
     3301 cgcgatttcg cgggcccggg gctcgacctg gccaacgttc gtctcatcga aacccgcatt
     3361 ttggtccggc gcgagcaagg ctgggaagcg ttgccctatg tgtggaacgc cgaccaaacg
     3421 gacgctgagc tggcgcgcac cggcgatgaa gtgtccttgg aactcgtggc ttcgggcgaa
     3481 gcgaacgagc cgttcacgta cgtggtgccg aacgaggtgc agtgcgcagg ttgtcatgtc
     3541 gcggatttga aagcgaagag catcgccccc atcggcctca aagcacgcca tcttaaccgt
     3601 ctgtacacct acgccacggg cgccgagaat cagttgagtt acctgcagaa ggtcggctac
     3661 ttggcgggct tgccggcact cgccgatgtg ccgcgcaatg ccaattggcg tgatgccgcc
     3721 cagccgctcg atgcccgcgc gcgggcctac ctcgatatca actgcgcgca ttgccacagc
     3781 gccaccggcc ccgcggacac aacagggctc acctttgagt cgatcacggc cacggatcgg
     3841 catctcggta tttgcaagcc gccggttgcg gcggggcgag gcaccggcga tcacctgtac
     3901 gacattgtgc
//
LOCUS       DASWZT010000235         2287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_79230, whole genome shotgun sequence.
ACCESSION   DASWZT010000235 DASWZT010000000
VERSION     DASWZT010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79230"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="VGE92_06490"
     CDS             <1..1001
                     /locus_tag="VGE92_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313503.1"
                     /translation="LAAGLNPGVATLFVQSKVPEHAELHLLLSMITPIGWLERVPSYK
                     DQQAQLAERDLSSYGFLGYPLLQSADILMYRSQYVPVGEDQVAHVEITREVARRFNHV
                     YGREPEFEQKVEKAIKSLGSRNATMYRNLRREFQEKGNVDALAKARAMLESNARLTVA
                     DRDRLFGFLEGTGIAILPEPEVLLTATPKVPGLDGRKMSKSYGNTIGLREDPDSVAKK
                     LKAMQTDPARARRTDPGDPDKCPVWDLHKVYSNADTQAWVQQGCRSAGIGCLECKAPL
                     IEKIVEEISAISKRAQEFEENPELVRGIIAEGCERARAAARDTLDAVRQAMGMDYR"
     gene            998..1912
                     /locus_tag="VGE92_06495"
     CDS             998..1912
                     /locus_tag="VGE92_06495"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEY1313504.1"
                     /translation="MKPELTLVQKTAPPPAAPVPQPAQGEMPFAVVEGQPVTEMPRDL
                     YIPPQALEVFLEAFEGPLDMLLYLIRRQNLDILDIPIAEITRQYMRYIELMQVLQLEL
                     AGEYLLMAATLAEVKSRMLLPRIGTDEAAEESDPRAELVRRLQEYERFKRAAEGIDRM
                     PRLDRDTWMASADLVDRKVVRIPPQVTLQEMLLAFQDVLARSDMFAHHHVQREPLSVR
                     QRMTDVLAALSASAFIDFAQLFRAEEGRRGVTVTFVAVLELLREGLIEIVQAEAYAPL
                     HVRLGNPARHLTLVADDGAANDDLARTQ"
     gene            1909..>2287
                     /locus_tag="VGE92_06500"
     CDS             1909..>2287
                     /locus_tag="VGE92_06500"
                     /inference="COORDINATES: protein motif:HMM:NF016005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEY1313505.1"
                     /translation="MNDQYVKNVIEAAMLAAGRPLSMDELVNVFDERDGSNAEEVRNA
                     IAALKVEYETRGLELLEVASGYRIQIKAGVAEPVSRLWQERPAKYSRALLETLALVAY
                     RQPITRGEIEQIRGVTVNPNIIKT"
BASE COUNT          455 a          652 c          776 g          404 t
ORIGIN      
        1 ggctggcggc gggtctcaat cccggtgtgg cgacgctgtt cgtgcaatcg aaagtgcccg
       61 agcacgcaga attgcatctg ctgctatcga tgatcacgcc catcggctgg ctcgagcggg
      121 taccgtcgta caaggatcaa caagcgcaat tggcagagcg cgacttgagc agctacgggt
      181 ttctgggcta cccgttgctg caatcggcgg atattttgat gtatcgctcg caatacgtac
      241 cggtgggcga ggaccaggtt gcgcatgtgg agatcacgcg cgaggtcgcg cggcgtttca
      301 atcatgttta cggccgcgag ccggagttcg agcagaaagt cgagaaagcc atcaagagtc
      361 tgggttcgcg caatgcgacc atgtaccgca acttacgcag ggagtttcag gaaaaaggca
      421 atgtcgacgc gctcgccaag gcgcgcgcca tgctcgaatc caatgcgcgc ttgacggtcg
      481 ccgaccgcga ccggctgttc ggatttctgg aaggcacggg catcgcgatt ttgcccgagc
      541 cggaagtgct gctgaccgcc acccccaagg tgccgggact cgacgggcgc aagatgtcga
      601 agtcctatgg caataccatc ggcttgcgcg aagatcccga ctcggtcgcg aagaagctca
      661 aggccatgca aaccgatccg gcgcgcgcgc ggcgcacgga tcccggcgat cccgacaaat
      721 gtccggtgtg ggatttgcac aaagtgtatt caaacgccga cacccaggcg tgggtgcagc
      781 aaggttgtcg cagcgccggt atcggctgct tggaatgcaa ggcgccgctg atcgagaaga
      841 tcgtcgagga gatctccgcc atctcgaagc gggcgcaaga gtttgaggag aatccggaac
      901 tggtccgggg tatcatcgca gaagggtgcg agcgggcgcg cgcggcggcg cgcgatacgc
      961 tcgacgccgt ccgtcaagcc atgggcatgg attatcgatg aaacctgaat tgacgcttgt
     1021 gcaaaaaacc gcgccgccgc cggcggcgcc ggtgccgcag ccggcgcaag gggagatgcc
     1081 gtttgcggtc gtggaaggtc agcccgtgac cgagatgccg cgagatttgt atatcccgcc
     1141 gcaggcgctc gaggtgtttc tcgaggcgtt cgaggggccg ctcgacatgt tgctgtatct
     1201 gatacggcgg cagaacctgg acattctgga tattccgatc gccgagatta cgcgtcagta
     1261 catgcgctac atcgaactga tgcaggtgct gcaattggaa ttggcgggcg agtacttgtt
     1321 gatggccgcg accttggccg aagtgaagtc gcggatgctg ctgccgcgga tcggcacgga
     1381 tgaagcagcg gaggagagcg atccgcgcgc cgagctggtg cgccgcctgc aggagtacga
     1441 gcgcttcaaa cgcgcggcgg agggcatcga tcggatgccg cggctcgatc gcgacacgtg
     1501 gatggcgtcg gcggatctgg tcgatcgcaa ggtcgtgcgc ataccgcccc aggtgacgct
     1561 gcaggaaatg ctgctggcct tccaggacgt gctggcgcgc agcgacatgt tcgcgcacca
     1621 tcacgtgcag cgcgagccgc tgtcggtgcg ccaacggatg accgacgtgc tggccgcgct
     1681 cagcgccagt gcgttcatcg attttgcaca actatttcgt gccgaggaag gccgccgcgg
     1741 cgtgacggtg accttcgtcg ccgtattgga actgctgcgc gaagggttga tcgagatagt
     1801 ccaggcggag gcgtacgcgc cgctgcacgt gcgcctcggc aacccggccc ggcacctaac
     1861 tctggtggcc gacgatggcg ccgccaatga cgaccttgcg agaacccaat gaacgatcaa
     1921 tacgtgaaga atgtgattga agcggccatg ctggcggccg ggcggccgtt gtccatggac
     1981 gaattggtga acgtattcga cgagcgcgac ggatccaatg ccgaggaggt gcgcaatgcc
     2041 atcgccgcgc tcaaggtgga gtacgaaacg cgcggtctgg agctgctcga agtggcgagc
     2101 ggttatcgaa ttcaaatcaa ggcgggcgtc gcagagccgg tgtcgcggct gtggcaggag
     2161 cggccggcga agtattcgcg tgcgttgctc gaaaccctgg ctttggttgc ctaccgccag
     2221 ccgatcacac gcggcgagat cgaacaaatc cgcggcgtca cggtcaatcc caacatcatc
     2281 aagacgc
//
LOCUS       DASWZT010000236        14793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_55103, whole genome shotgun sequence.
ACCESSION   DASWZT010000236 DASWZT010000000
VERSION     DASWZT010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14793
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55103"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="VGE92_06505"
     CDS             complement(<1..1226)
                     /locus_tag="VGE92_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY1313506.1"
                     /translation="MKISMLQRRAFLAVPLFGILLACAAARAAQPSGAQSAAEDSDTP
                     AASAAAAASENSDWQDQSPPRRHRHSHGEGGDVVNIGHDSTLPQGQKSDSVVSVFGSS
                     SSEGETDDVVAVFGDTRATGIVHDNVVSVFGNVYIDGKLEGDAVAVLGNMELGPHAEI
                     DGDVFAIGGTLRRDPAAIVHGGAQDYFALDFGGAGWLRTWIHHCLLYGRPLALVPGLG
                     WAWGLAFSFLALYAALALLFRDGITRCVQTFESQPGQSVLAALMAMLLTPVLVVLLFV
                     TVIGIAAVPFVVFGLFCAGLFGKAVMLAWLGRRVTDKREGPMSHPVIAVLIGGVLVTA
                     LYLIPVLGFIIYKLLGVLGLGAVVYTLILAARAHHAAKEPPRPVPPAGAAPASALGAS
                     AASSAAPAAGASAASSA"
     gene            complement(1223..1675)
                     /locus_tag="VGE92_06510"
     CDS             complement(1223..1675)
                     /locus_tag="VGE92_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313507.1"
                     /translation="MTPHDPDEALALLAQTLRELPLRRAPATLEPRVIGELQRRAALP
                     WWRRSFAHWPLAARCVFVVICAGLVLGALGGTAAVANVHSLSWLREAGGLLMSLGYVA
                     ALLEHVTLPSWVYGAIAVGAVLYAVLFGLGAAVYRTLYLPPPVVESNI"
     gene            complement(1672..2205)
                     /locus_tag="VGE92_06515"
     CDS             complement(1672..2205)
                     /locus_tag="VGE92_06515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY1313508.1"
                     /translation="MQHPPIGPNRSDEFTTFMRAYQDMVFSTASRLAGDDGQAQDIAQ
                     EVFLRAYDHFEDLRTSPTAGGWLKTVTTNLTLNYLTRYRKRWRMFSELPEQEFTAPQP
                     DNLLADLSAEQQRGLIDEALRKLPEHQRLPLVLYHFEELTYEEIAIKLHVSLAKVKTD
                     IRRARAALLPLLHRDGP"
     gene            complement(2289..2873)
                     /locus_tag="VGE92_06520"
     CDS             complement(2289..2873)
                     /locus_tag="VGE92_06520"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY1313509.1"
                     /translation="MAFVAPLLLHGEHVSLVPLTHSHHDDLVEAARDGELWTLWYTTI
                     PKPEGMRAEIDRRLGLQKQESMLPFAVIEKSSGQAVGMTTFMHIDAANRRLEIGSTWY
                     RKRVQRSPLNTECKLLLLTHAFEQLSCIAVEFRTHFFNHQSRAAIERLGAKLDGILRQ
                     HAFAGNGTLRDTCVYSILDREWPTVKANLVYRLE"
     gene            2872..3237
                     /locus_tag="VGE92_06525"
     CDS             2872..3237
                     /locus_tag="VGE92_06525"
                     /inference="COORDINATES: protein motif:HMM:NF005297.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor Sui1"
                     /protein_id="HEY1313510.1"
                     /translation="MSHNTNMNARIVYSTGVGSLCPNCRRPVRDCVCPKGVAGAAKPA
                     TVRVGREVKGRAGKGVTTVTGLPLAVADIESLATQLKKRCGSGGTVRAGVIEIQGDHR
                     DVIVAELNKLGWPAKRSGG"
     regulatory      3288..3379
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3517..4590
                     /gene="gcvT"
                     /locus_tag="VGE92_06530"
     CDS             3517..4590
                     /gene="gcvT"
                     /locus_tag="VGE92_06530"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEY1313511.1"
                     /translation="MGLKTALYDTHLEEGAKIVDFGGWDMPLHYGSQIEEHHAVRRDA
                     GMFDVSHMGVVDLRGARVREFLQSLLANDVGRLKSEGKALYSCMLLPSGGVIDDLIVY
                     SMSDTWFRMVVNAGTRDKDLVWIRRHAAAFGVSVLEHHDLAMIAVQGPTAREKTLAVL
                     PEAVRAAARQLAPFSGAAFEPWFIARTGYTGEDGFEVTLPAGDAAQTWRALRAQGVKP
                     AGLGARDTLRLEAGMNLYGNDMDEDHHPLESGLAWTVAFEPKERDFIGRQALERLRGA
                     GLELVGLVLEERGVLRSHQKVLPSGEVTSGTFSPTLNRSIALARVPKTGAGRVQVEIR
                     GKAHAARIVKPPFVRHGKALISL"
     gene            4607..4999
                     /gene="gcvH"
                     /locus_tag="VGE92_06535"
     CDS             4607..4999
                     /gene="gcvH"
                     /locus_tag="VGE92_06535"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEY1313512.1"
                     /translation="MSNIPNDLRYTKSHEWLRAASDGSVEVGISDHAQSALGDLVFVE
                     VPEVGRALRAGEACAVVESVKAASDVYSPVSGKVTASNSTLATQPELLNHEPYGGGWL
                     FRLHPDVPVDGAALMSAADYAKYVAEEA"
     gene            5008..6372
                     /gene="gcvPA"
                     /locus_tag="VGE92_06540"
     CDS             5008..6372
                     /gene="gcvPA"
                     /locus_tag="VGE92_06540"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEY1313513.1"
                     /translation="MAFIPHTPDDIAGMLEVIGARSIDDLFDEIPKNLRAGTLGVAPA
                     MGEMEVVRLLKGRAASDGRPMNFIGAGAYEHHIPAPVWAITTRGEFYSAYTPYQAEAS
                     QGTLQVIYEFQSMICALTGMDVSNASLYDGASALAEACLMAVRANRAANSRRILLPRA
                     LNPTYAQVAQSIAGNQGLTFESVDFDRATGCTLLESLEAYGGQDVTALVIQQPNFFGN
                     LEDCQVLAEWAHRNKMLVIAVVNPISLALLKAPGEWGADIVCGEGQPLGVPLSAGGPY
                     FGFMATRTQYVRQMPGRIVGRTVDVEGRPGFSLTLQAREQHIRRSKATSNICTNQGLM
                     VTAATIYMSLLGAAGLMRVAAASLQRTADLVAALGKIAGVQPAFAAPRFHEAVLLLDR
                     PVAPILAALARRGILGGLDLAERYPELGHALLVCATETKLDVDIDAYAGALADIMNPS
                     RAAA"
     gene            6382..7851
                     /gene="gcvPB"
                     /locus_tag="VGE92_06545"
     CDS             6382..7851
                     /gene="gcvPB"
                     /locus_tag="VGE92_06545"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEY1313514.1"
                     /translation="MSDLPAREPLIFELSKPGRRAGTQFPPHVAGSAEIPAHLRRASA
                     PALPEVSELQVVRHFTRLSQMNFSIDTQFYPLGSCTMKYNPKVCNSLAMLPEFLERHP
                     LGPEPQGFLACMYELQEMLQEITGMQSVSLTPMAGAQGEFAGVAMIRAYHRARGDLAR
                     SEIIVPQAAHGTNPATAAMCGCSVKEIPCDANGDVDIEALQAAVGPATAAIMLTNPST
                     LGVFEHKITEVARIVHEAGGLLYYDGANLNAILGKVRPGDMGFDVIHMNLHKTFSTPH
                     GGGGPGSGAVGVGARLRPFLPIPIVGKDGARFRWLTERDVPQTIGRLSGFMGNAGVNL
                     RAYIYMRLLGRDGMVRVAEFAALNANYLLKRLQAAGFEAAYPARRASHEFIITLKREN
                     RLYEVTAMDVAKRLLDYGFHAPTTYFPLLVPECLLIEPTETEAKEDLDRFCDAMIAIR
                     REMEHEPEKLSSAPHTMPVRRLDDVRAAKQLDLAWKPAA"
     gene            7852..8226
                     /locus_tag="VGE92_06550"
     CDS             7852..8226
                     /locus_tag="VGE92_06550"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEY1313515.1"
                     /translation="MSDRRKPTGITRVLRAFSYSFQGFRYAWREEAAFRQEVGLSIAV
                     VAAGLYLGRTGVERALLVCPMLLILVVEILNSAIEAVVDRSGTERHPLAGMAKDMGSA
                     AVMLSFVLLGTVWFLILSDHGQ"
     gene            8249..11137
                     /locus_tag="VGE92_06555"
     CDS             8249..11137
                     /locus_tag="VGE92_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY1313516.1"
                     /translation="MPRIALFLSVALSVSAVLFAPVFAAVPAVAAPAPVPAPGIPSSP
                     ANWAQTLERIASGVVTIQIDSTRAFDTEWNTTAQATGFVIDAKRGLILTNRHVVTPGP
                     VTAQAIFLNREEVQLYPVYRDPIHDFGIYRYDPAKLKFIQPAELPLYPAGAVIGREIR
                     VVGNDAGEQLSILAGTLARLDREAPEYGYGKYNDFNTFYFQAASSTSGGSSGSPVIDI
                     EGRVVALNAGGATGAASSFYLPLTAVTRALKYIDAGKPVPRGTLETVFKYTPYDELRR
                     LGLNADTEAKLRSAYPKLTGMLVVDDVQPGSGADGVLAPGDILVAIDGKPIPEFFSLE
                     DVLDSRVGQQINIEVQRGRDTLRHALEVQSLNAITADEYIEFGEAVVHTLSYQQARHF
                     NVAIKGVYVANPGYVFGSAGIPRAALINTFNGKKVENLKDFEAALADLADGASAPVRY
                     ATLEDPRAAQLKVIRMDRRWFPARKCKRDDTLGTWPCVSISRGPAAAAEKPASTQFAK
                     TGDPRIDRLAPSLVMVNFDMPYSVSGVTEKNYHGTGVIVDAERGLVAIDRNTVPIAMG
                     DVRITFAGTVEVPGRVVFIHPLHNLAVVAYDPKSIGSTPVRAATLLTKPLAAGDNVWV
                     VGQRADSKILSQKTQVSSVDVVAFPLSRTLRFRDSNLEAISLINPPGDYDGVLADDKG
                     DVLALWSSFAFESQRELEQVNRGMPAELVSEMIGVVADGRPLYSFEAEFNVQSLAGAR
                     ELGLPDEWVHRFEEHNSQRRQVLGIDRLVAGSPAAALLEPGDLVLAIDGKVVNRFREV
                     ERAVQKPRVMVSVWRDGAEKTLEMKTVGLDGRDIDRVLIWAGATLQAPHRAMAAQRGI
                     APYGVFVSFFFYGSPATHYGLYAGRRITEVDGQPTADLDAFIKVVSGKPDRASLRLKT
                     ISWNGQTDVITLKLDKHYWPAYELERQKDGSWIRTALDPPEASAATAP"
     gene            11170..11697
                     /locus_tag="VGE92_06560"
     CDS             11170..11697
                     /locus_tag="VGE92_06560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1313517.1"
                     /translation="MNQASAIDQACRADLPQILAIYNEVIRNSTAVYQEEDFTPARGA
                     AWFEAKAERGFPLVVTRDASGVTGFGTFGDFRAWPCYRYSVEHSVHVRADYRGRGIGR
                     ALVQDLMTRATDLGKHVMIAGIDADNAVSIGLHRSLGFDTVGYFHQVGFKFGRWLDLV
                     FMQRILLQTGVALAK"
     gene            11805..12797
                     /locus_tag="VGE92_06565"
     CDS             11805..12797
                     /locus_tag="VGE92_06565"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY1313518.1"
                     /translation="MVRATQTEIEHAVEALRAGELVVFPTETVYGLGANAANPAAVRK
                     IFEVKGRPPDHPVIVHLDDPRYLHRWVSSVPPVAEQLAARFWPGPLTLILPKADNVND
                     IVTGGQDSIGIRVPSHPMAQQLLTAFGGGIAAPSANRYGRLSPTKPEHVRDELGSAVK
                     IMLDGGDSPIGLESTIVSCLDNQARLLRPGFITRSQLEQAVGSLAVGGMVPRAPGDRA
                     LHYAPSTPLEIVSSDDLEVRAGEVTAREEKVAVLALRPPLQTQRHMTWINAGKRPDVY
                     AHNLYNHLRTLDRAGCVKIFVQGLPQDERWAAILDRLQRASGMGGDDSLEMSVS"
     gene            12797..14005
                     /locus_tag="VGE92_06570"
     CDS             12797..14005
                     /locus_tag="VGE92_06570"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HEY1313519.1"
                     /translation="MAKPLLKTGSARDAGLELDPARDPFAPESIKHLVSRYGSPLFII
                     DAERVRRQYRRLAAALPRVDLHYALKPLPHASVISTLAAEGACFDLATNGEVELVRRL
                     KIAPQRCIHTHPIKRDSDIRTALAYGVNRFVVDNVDELRKFVKFRTRSSLLIRVSFSS
                     PEARCDLSRKFGCEPEAAVDLFRLAAQLNIKIDGLSFHVGSQAAGPAMFVEAIARCAQ
                     LLASAGERGHAPSILDIGGGFPVDYLERCMPIEEFCAPIAAALAALPAGIRVIAEPGR
                     YISAPAAITAAQVMGRAQRDGRWWYYLDDGLYGSYSGQVYDHARYPVEALVAAGKTYP
                     SVLAGPTCDSIDVINDHLELPMLKIGDLVIGHAMGAYTWASASEFNFFPRATVLALDR
                     GRLLTDGSCP"
     gene            14002..14550
                     /locus_tag="VGE92_06575"
     CDS             14002..14550
                     /locus_tag="VGE92_06575"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HEY1313520.1"
                     /translation="MTWSRRRVLLSGSALAGLSALTSVARSTAAQRPAPKRIALANLH
                     TNERLEVEYCRDGRYLPEALKAIEAVLRDFRSGEQHPIDPKLMDYLIDVAHAAGADPA
                     FSVISGYRSPQTNARLRETSTGVAQRSLHMEGRAIDVRMAGVDCARLAAHAEELKHGG
                     VGYYRASDFVHLDTGAFRTWKG"
     gene            complement(14570..>14793)
                     /locus_tag="VGE92_06580"
     CDS             complement(14570..>14793)
                     /locus_tag="VGE92_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313521.1"
                     /translation="YVLRDSPQWTREKILAAMNGTQPVTVILKNHIRVFILYGTAIAT
                     ERGNILFFDDIYKHDARLEKALDARRVRT"
BASE COUNT         2701 a         4632 c         4838 g         2622 t
ORIGIN      
        1 gcactcgaag cggctgaggc gcccgccgcg ggtgcggcac tcgaagcggc tgaggcgcca
       61 agtgcggacg cgggcgccgc accggccggc ggcaccggcc gcgggggctc cttggcggcg
      121 tgatgcgcgc gggcggccaa aatcaaggta tagaccacag cgcccaaccc gagcacgccg
      181 aggagcttat aaatgataaa tccgagcacc ggtatcaagt acaacgccgt gaccagcacc
      241 ccgccgatga gcacggcgat gaccgggtga ctcatcgggc cctcgcgctt gtccgtcacg
      301 cgccgcccca gccacgcgag catcaccgct ttgccgaaca ggcccgcgca aaatagtccg
      361 aacacgacaa acggtacggc ggcaatgccg atgacggtga cgaacaacaa cacgaccaat
      421 accggcgtca gcagcatggc catgagcgca gcgagcaccg attgacccgg ctggctctcg
      481 aaggtctgca cgcagcgggt gatgccgtca cggaacagca gggcgagcgc ggcgtaaagc
      541 gccaagaaag agaacgccaa cccccacgcc cagccgaggc cgggcaccag ggccagcggc
      601 cgtccgtaca gcaagcaatg atgaatccac gtacgcagcc acccggcgcc cccgaagtcc
      661 agggcaaagt agtcctgcgc gccgccgtga acgatcgccg ccgggtcgcg tcgcagcgtg
      721 ccgccgatgg cgaagacatc gccgtcgatc tcggcatgcg gtccaagctc catgttgccg
      781 agtaccgcga cggcgtcgcc ctcgagtttg ccgtcgatgt acacattgcc gaagaccgac
      841 acgacgttgt cgtgcacgat gccggtggct cgcgtatctc cgaagaccgc gaccacatcg
      901 tcggtctcgc cttcgctgct cgaggaaccg aagacggaga cgaccgaatc cgatttctgc
      961 ccttgcggca gagtcgaatc gtggccgata ttcacgacat caccgccctc gccgtggctg
     1021 tgccggtggc ggcgtggggg ggattgatcc tgccagtccg agttctccga ggccgcagcg
     1081 gctgcggagg cggccggtgt gtcgctgtct tcagcggcag actgggcgcc ggacggttgc
     1141 gccgcgcgtg ccgccgcgca agccagcaag atgccgaaga gcggcacggc caggaacgcg
     1201 cgccgttgca gcatggagat tttcatatgt ttgactcgac taccggtggc ggaagataga
     1261 gcgtgcggta caccgcggca ccgagcccga acaacactgc atacagcacc gcgcccacgg
     1321 cgatggcgcc gtaaacccag gacggaagcg ttacatgctc gagcaacgct gcgacataac
     1381 ccagcgacat gagaagtcca cccgcctcgc gcagccatga cagggaatgc acgttggcca
     1441 ctgccgccgt gccgccgagc gcacccaaca ccaaaccggc gcagatcacg acgaagacgc
     1501 agcgtgcggc cagcggccag tgcgcgaagc tgcgtcgcca ccagggaagc gcggcgcgcc
     1561 gttgcagttc gccgattacg cgcggctcga gcgtcgccgg cgcgcgccgc aacggcaact
     1621 cgcgcagcgt ttgagcgagc agcgccagag cctcatcggg atcatgcggg gtcatgggcc
     1681 gtctcgatgc agcagcggaa gcaatgctgc gcgggcgcgc cgtatgtcgg ttttgacctt
     1741 ggccagcgag acatgcagct tgatggcgat ctcctcatac gtcagttctt cgaagtggta
     1801 cagcaccaga ggcagccgct gatgctccgg cagtttgcgc aaggcctcat cgatcagccc
     1861 gcgttgctgc tcggcgctca agtccgccag caggttgtcc ggctgcggcg cagtgaactc
     1921 ttgttccggg agttccgaga acatgcgcca acgcttgcga tagcgcgtca aataattgag
     1981 tgtcaaattg gtggtcacgg tcttgagcca gccgcccgcg gtcggactgg tgcgcagatc
     2041 ctcgaaatgg tcataggcgc gcaggaacac ctcctgggcg atatcctgcg cctgcccatc
     2101 gtcgccggcg agccgcgagg cggtcgagaa aaccatatcc tgataggcac gcatgaaggt
     2161 cgtgaactcg tcagagcggt tgggcccgat gggtggatgt tgcactggac tacattcgtt
     2221 tagatccaga acaccgccgg cctgaaaaag ttgcaggctc ggggcggcgc ctacgggaag
     2281 gcgggaggct actcgaggcg atacacgaga tttgccttaa cggtgggcca ctcccggtca
     2341 aggatgctgt acacgcaggt atcgcgcaag gtgccgttgc ccgcgaacgc atgctgccgc
     2401 agtatgccgt cgagcttcgc gcccaggcgt tcgatggcgg cgcggctctg gtgattgaag
     2461 aaatgggtgc gaaactccac ggcgatgcag gagagctgct cgaacgcgtg ggtcagcaac
     2521 agcagcttgc actcggtatt gagcgggctg cgctgcacgc gcttgcggta ccaggtcgaa
     2581 ccgatctcca gccgacggtt ggcggcatcg atgtgcatga atgtcgtcat accaaccgct
     2641 tgaccgctcg atttctcgat caccgcgaac ggcagcatcg actcctgttt ttgcagcccc
     2701 aggcggcggt cgatttccgc ccgcatacct tcgggcttcg gaatcgtcgt gtaccacagc
     2761 gtccaaagtt cgccgtcgcg cgcggcttcg accagatcgt cgtggtgcga atgggtgagc
     2821 gggacgagcg atacgtgctc gccgtgcaac aagagcggtg ctacaaaagc catgagtcat
     2881 aataccaaca tgaacgcgcg aatcgtctac tcaaccggag tcggcagtct ctgccccaat
     2941 tgtcgcaggc cggtgcgcga ttgcgtgtgc ccgaaaggag ttgcgggcgc cgcgaaaccc
     3001 gctacggtgc gggtgggccg cgaagtcaaa ggccgggcgg gcaagggggt taccaccgtc
     3061 accggtctgc cgctggccgt ggccgacatc gagtcattgg ccacacagct caagaagcgc
     3121 tgcggatctg gcggtacggt gcgcgcgggc gtgatcgaga ttcaaggcga tcatcgcgac
     3181 gtgatcgttg ccgagctcaa caaactgggt tggccggcga agaggtccgg cggctaggcg
     3241 cgccgcgcca ccgccgcaga cagatcgtgt atcatgtgga catcgcaata agagctgtct
     3301 ggagagagtc ctcgcgagga ggaccgccga aggcgcaacc cgcccggaaa cgctcaggca
     3361 catgaacggc agactctcgc gatctctgga gagcggtcgc agcggcgatc caccgaaggg
     3421 gagcagcgct cctaagcgcg cgatctctca ggttcaaagg acagaggggc aggcaccgaa
     3481 ggtgcttgtc tcttattctt gcgccgtgag gttgcgatgg gcttgaagac cgcgctctat
     3541 gacacccacc tcgaggaggg tgcgaaaatc gtcgatttcg gcggctggga tatgccgctt
     3601 cactatggtt cacagatcga agaacaccat gcggtgcgcc gcgacgccgg catgttcgac
     3661 gtctcgcata tgggtgtcgt cgatttgcgc ggcgcgcgcg tgcgcgaatt cctgcagagc
     3721 ctgctggcca acgacgtcgg ccgcctgaag agcgaaggca aagcgctgta ctcctgcatg
     3781 ctgctgccga gcggcggcgt gatcgacgat ttgatcgtct attccatgtc cgacacctgg
     3841 tttcgcatgg tcgtcaatgc cggcactcgc gacaaggatc tcgtgtggat tcgccgccac
     3901 gcggccgctt tcggcgtgtc ggtcctcgaa caccacgatt tggcgatgat tgccgtgcaa
     3961 ggcccgactg cccgggaaaa gacgctcgcc gtgttgccgg aggccgtgcg cgccgccgcg
     4021 cggcagctgg cacccttctc tggcgccgct ttcgaaccct ggttcatcgc gcgcaccggc
     4081 tatacgggtg aagacggttt cgaagtgact ctgccggccg gcgacgcggc gcagacctgg
     4141 cgagcgttgc gcgcgcaagg ggtcaagccc gcgggcttgg gcgcgaggga caccctgcgc
     4201 ctggaggccg gcatgaatct ttacggcaac gacatggacg aagatcatca cccattggag
     4261 tcgggattgg cgtggacagt ggccttcgag cccaaggagc gcgattttat cgggcgccag
     4321 gcgctcgagc ggctgcgcgg cgcaggtctc gaactcgtcg gcctggtgct cgaagagcgc
     4381 ggtgtgctgc gcagtcatca gaaggtattg ccgagcggcg aggtcacgag cggcacattc
     4441 tcgcccacct tgaatcgctc gatcgcgttg gcgcgtgtgc cgaaaaccgg tgccgggcgc
     4501 gtgcaagtcg agattcgcgg caaagcgcac gccgcgagaa tcgtcaagcc gccgttcgtg
     4561 cggcatggaa aggcgttgat ttcgctctag tcacggggga tccatcttga gcaacattcc
     4621 aaatgatctg cgatatacca agagccatga atggctgcgc gccgcctcgg acggcagcgt
     4681 cgaggtcggc atatccgacc atgcgcagag tgcgctcggc gatttggtgt tcgttgaagt
     4741 gccggaggtc ggccgcgcgc tgcgtgcggg cgaagcctgc gcggtggtcg agtccgtgaa
     4801 ggccgcctcc gatgtgtaca gcccggtgag cggcaaggtc accgcgagca attccacgct
     4861 cgcgacccag ccggagctgc tcaatcatga gccgtacggc ggcggatggt tgttccggct
     4921 gcaccccgat gtgccggtcg acggcgccgc gctcatgagc gccgccgatt acgcgaagta
     4981 cgtggcagag gaagcctagg agtctcaatg gcattcattc cgcatactcc cgatgacatc
     5041 gccggcatgc tggaagtcat cggcgcgcgc tcgatcgacg acctgttcga cgagattccc
     5101 aagaatctac gggccggcac gctcggcgtc gcgcccgcga tgggcgaaat ggaagtggtg
     5161 cgactgctca aggggcgcgc cgcgagcgat ggccggccga tgaatttcat cggcgcgggc
     5221 gcctatgaac atcatattcc ggccccggtc tgggcgatca cgacgcgcgg cgaattctat
     5281 tccgcgtata ccccgtacca agcggaggcg agtcagggca ccttgcaggt gatctacgaa
     5341 tttcaatcga tgatctgcgc cttgaccggc atggacgtat ccaatgcgtc gctgtacgac
     5401 ggcgcttccg ccttggccga agcgtgcctg atggcggtgc gcgccaatcg cgccgccaat
     5461 tcgcggcgga ttctgctgcc gcgtgcgctc aatccgacct acgcccaagt cgcgcagtcg
     5521 atcgccggca atcagggtct gacgttcgaa agcgtggatt tcgatcgggc gacgggctgc
     5581 acgctgctcg agtcgctcga ggcttacggc gggcaggacg tcaccgctct ggtgattcag
     5641 cagccgaatt tcttcggtaa tttggaagac tgccaagtgc tcgcggagtg ggcgcatcga
     5701 aacaagatgc tggtgattgc cgtggtcaac cccatctcgc tggcactctt gaaagcgccc
     5761 ggggagtggg gcgcggacat cgtatgcggc gaaggccagc cgctcggcgt gcccctgtct
     5821 gcgggcggcc cgtatttcgg attcatggcg acgcgcacgc agtacgtgcg gcaaatgccg
     5881 ggacgcatcg tcggccggac cgtggatgtg gaaggccgtc cgggattcag tctgaccctg
     5941 caggcgcgcg agcagcacat caggcgcagt aaagcgacct ccaacatctg caccaaccag
     6001 ggcttgatgg tgaccgccgc aacgatttat atgtcgctgc tcggtgcggc cggcctcatg
     6061 cgcgtggcgg cggccagcct gcagcgcacc gccgatttgg tcgcggcgct cggcaaaatc
     6121 gccggcgtgc agccggcctt cgcagcgccg cgctttcacg aagccgtgtt gctgctggat
     6181 cggccggtgg caccgatcct cgcggcactg gcgcggcgcg gcatactcgg cgggctcgat
     6241 ctggcggagc gctatccgga actcggccac gcgcttttgg tgtgcgcgac cgaaacgaaa
     6301 ttggacgtcg acatcgatgc ctatgccggc gcgctcgccg atatcatgaa tccatcacgc
     6361 gccgccgcct aggagaacgc gatgagtgat ttgccagcga gagaacccct gatattcgaa
     6421 ctcagcaagc cgggacggcg ggccggcacg cagtttccgc cgcacgtcgc cggtagtgcc
     6481 gaaatcccgg cgcacctgcg ccgggcgagc gcgccggcgc tgccggaagt atccgagttg
     6541 caggtcgtgc ggcatttcac gcgcctgtcg cagatgaatt tctcgatcga cacgcagttc
     6601 tatccgctcg ggtcgtgcac catgaagtac aaccccaagg tgtgcaacag cttggcgatg
     6661 ctgccggaat ttctcgagcg acacccgctc gggcccgagc cgcagggatt tctcgcctgc
     6721 atgtacgagc tgcaggagat gctgcaggaa atcaccggca tgcagagcgt cagcctcacg
     6781 ccaatggccg gggcgcaggg cgagtttgcc ggcgtcgcga tgatccgcgc ctatcatcgg
     6841 gcccgtggcg acttggcacg cagcgaaatc atcgtgccgc aggccgcgca cggcacgaat
     6901 cccgccaccg cggccatgtg cggctgcagc gtcaaagaga ttccctgcga tgcgaacggc
     6961 gatgtcgata tcgaggcgct gcaggccgcg gttggacccg ccacggcggc catcatgctg
     7021 acgaatccat cgacgctcgg cgtgttcgag cacaagatca ccgaggtcgc gcgcatcgtc
     7081 catgaggcgg gcggcctgct ctactatgac ggcgccaatt tgaacgccat tctcggcaag
     7141 gtgcgacccg gcgacatggg attcgacgtc atccacatga atctgcacaa gacgttctcg
     7201 acgccgcacg gcggcggcgg tcccgggtcc ggagcggtcg gcgtgggtgc ccgcctgcgg
     7261 ccgttcttgc cgataccgat cgtaggcaag gacggtgcgc ggtttcgttg gttgaccgag
     7321 cgcgacgtgc cgcagacgat cggacgattg tcgggcttca tgggcaacgc cggggtcaat
     7381 ctgcgcgcct atatctacat gcgcctgctg ggccgcgacg gcatggtccg ggttgccgaa
     7441 ttcgcggcgc tcaatgcgaa ctacctgttg aagcggctgc aagccgcagg gttcgaagcg
     7501 gcctatccgg cgcgccgcgc gagccacgaa ttcatcatca cgctgaagcg cgagaaccgg
     7561 ctctacgagg tcaccgccat ggatgtggcg aagcggctgc tcgactacgg ttttcatgcc
     7621 ccgaccacct attttccctt gttggtgccc gagtgcttgt tgatcgagcc caccgaaacg
     7681 gaagccaagg aggacctgga tcggttctgt gacgcgatga tcgcgatccg gcgggaaatg
     7741 gagcacgagc ccgagaaact ctcatcggct ccgcatacaa tgccggtgcg gcgtctggat
     7801 gacgtacgcg ccgcaaaaca gctggatctt gcatggaaac ccgccgcttg aatgagcgat
     7861 agacgcaaac cgaccgggat cacacgcgtc ctgcgggcat tcagttattc tttccaaggt
     7921 ttccgctacg cctggcgaga ggaggctgca tttcgccagg aggtgggctt gtcgatagcg
     7981 gtggtcgccg ccgggctgta cttgggccgg acgggtgtgg agcgcgcgct gctggtctgc
     8041 cccatgttgc tgattttggt ggtcgagatt ctcaatagtg cgatcgaagc agtcgtcgat
     8101 cggtccggta ccgagcgtca tcctctggca ggaatggcca aggacatggg ttcggcggcc
     8161 gtgatgctga gttttgtctt gttaggaacc gtttggttcc tgatcttgtc cgaccacggg
     8221 caataatcac actaacccta ggatcggcat gccccgtatc gcgttgtttc tgtccgtcgc
     8281 attgtccgtc agcgctgtcc ttttcgcccc cgtgttcgcc gcggttcccg cggtggccgc
     8341 ccccgctccc gtgcccgcgc cgggcattcc gagttcgccg gcgaactggg cgcagaccct
     8401 ggagcgcatc gcgagcggcg tcgtcacgat tcaaatcgac agcacgcggg cgttcgatac
     8461 cgagtggaat accacggcac aggccacggg cttcgtcatc gacgccaaac gcggactcat
     8521 tttgacgaat cgacacgtcg tgacgccggg cccggtcacc gcccaagcca tttttctgaa
     8581 tcgcgaggaa gtccagctgt acccggtgta ccgcgatccc atccacgact tcggcatcta
     8641 tcgctacgac ccggcaaaac tgaaattcat ccagccggcg gaactgccgc tatatccggc
     8701 gggcgccgtc atcgggcgcg aaatccgcgt ggtcggcaac gacgccggcg aacagctgtc
     8761 gatactcgcc ggcaccttgg cgcgtctgga tcgcgaggct ccggaatacg ggtacggcaa
     8821 gtacaacgac ttcaatacat tttattttca ggctgcctcg agcacctcgg gcggctcctc
     8881 cggttcgccg gtcatcgaca tcgagggccg ggtggtcgcg ctcaatgccg gtggagcgac
     8941 cggcgccgcg tcgagctttt atctgccgct gaccgcggtg acgcgcgcgt tgaaatacat
     9001 cgacgccggc aagccggtgc cgcgcggcac gttggagacc gtgttcaaat acacgcccta
     9061 cgatgagctg cggcgcttgg ggttgaacgc cgataccgaa gccaagctgc gcagcgccta
     9121 cccgaagctg accggcatgc tggtcgtcga cgacgtgcaa cccggatccg gagccgacgg
     9181 cgttttggcg ccgggcgata tcctggtggc gatcgacggc aaacccattc ccgagttttt
     9241 ctcgctcgag gacgtgctcg acagccgtgt cgggcagcaa atcaacatcg aggtgcagcg
     9301 cggccgcgac accctgcggc atgcgctcga ggtgcagtcg ctgaacgcga tcacggccga
     9361 tgaatacatc gaattcggcg aagccgtggt gcatacgctg tcctaccagc aggcgcggca
     9421 tttcaacgta gccatcaagg gcgtgtacgt cgcgaatccg ggctatgttt tcggcagtgc
     9481 cggcattccg cgcgccgccc tcatcaatac attcaacggc aagaaggtcg agaacctcaa
     9541 ggatttcgag gccgcgctcg cggatctcgc cgacggcgcg agcgccccgg ttcgctatgc
     9601 aaccttggaa gacccgcgcg ccgcgcaact caaggtgatc cgcatggatc ggcggtggtt
     9661 tccggcgaga aaatgcaagc gcgacgacac actcggaacc tggccatgcg tcagcatcag
     9721 ccgcggaccc gcggcggcgg ccgagaagcc cgcctcgacg cagttcgcca agaccggcga
     9781 tccgcgcatc gatcggctgg cgccctcgct cgtgatggtg aacttcgaca tgccgtattc
     9841 ggtgtcggga gtcaccgaga agaattatca cggcaccggc gtcatcgtcg acgccgagcg
     9901 cggcctggtg gcgatcgacc gcaacaccgt accgatcgcg atgggcgatg tgcggatcac
     9961 tttcgccggc acggtcgaag tgcccggcag agtggtgttc atccacccgc tgcataatct
    10021 cgcggtggtc gcttacgatc cgaagtcgat cggcagcacg ccggttcgcg cagctacgct
    10081 gctcaccaaa ccgttggccg ccggggacaa tgtctgggtg gtcgggcagc gcgcggattc
    10141 gaagattctg tcgcagaaaa cccaggtttc cagcgtcgat gtggtggcat ttccgttgtc
    10201 ccgcacgctg cgatttcgcg acagcaacct cgaggcgatc agcctcatca atccgccggg
    10261 agactacgac ggcgtgctgg cggacgacaa aggcgacgtc ctcgcgctgt ggtcgagttt
    10321 tgcgttcgag tcgcagcgcg aactcgagca ggtgaaccgc ggcatgccgg ccgagctcgt
    10381 ctcggaaatg atcggggtgg tcgcagacgg ccggccgctg tattcgttcg aagcggaatt
    10441 caacgtgcag tccttggcgg gcgcgcgcga gctcggatta ccggacgagt gggtgcaccg
    10501 cttcgaagag cacaactcgc agcggcggca agtcctcggc atcgacagac tggtggccgg
    10561 atcgcccgcc gcggcactgc tcgagcccgg cgatctcgtg ctcgcgatcg acggcaaggt
    10621 ggtcaaccgc tttcgcgaag tcgagcgcgc cgtgcaaaaa cccagggtca tggtcagcgt
    10681 gtggcgcgac ggcgccgaaa agacgctgga gatgaaaacg gtggggctcg acggccgcga
    10741 tatcgatcgc gtcctcatct gggccggcgc cacgctgcaa gctccgcacc gcgccatggc
    10801 ggcgcaacgc ggcatcgcgc cctatggcgt attcgtatcg ttcttcttct acggctcgcc
    10861 ggcgacgcat tacggattgt acgcgggccg ccgcatcacc gaagtcgacg gacaaccgac
    10921 cgccgatctc gatgccttca tcaaggtggt cagcggcaag cccgaccgag cgtcgttgcg
    10981 gttgaagacc atcagttgga atggtcaaac cgatgtgatt accctgaaac tcgacaagca
    11041 ctattggccg gcgtacgaac tcgaacggca gaaggacggc agttggatcc gcacggcact
    11101 cgaccccccg gaggcgagcg ccgcgacggc gccctgactg cgcgcctagc atcggtgcga
    11161 ggtgatgagg tgaatcaagc gagcgcgatc gatcaggctt gccgcgcgga tttgccgcaa
    11221 atcctggcca tttacaacga agtgattcgc aattcgaccg cggtctacca ggaggaggac
    11281 ttcacgccgg cgcgcggcgc ggcgtggttc gaggcgaagg cagagcgggg ttttcccttg
    11341 gttgtgacgc gcgatgcgtc gggtgtcacc ggcttcggca cgttcggtga ttttcgcgcg
    11401 tggccctgct atcggtacag cgtcgaacac agcgtgcatg tgcgggcgga ttaccggggc
    11461 cggggaatcg ggcgcgcgtt ggtgcaggac ctcatgacgc gcgccacgga tctgggcaag
    11521 cacgtgatga tcgccggcat cgatgcggac aatgcagtgt cgatcggcct gcatcgcagt
    11581 cttggatttg acactgtcgg gtactttcac caggtgggat tcaaatttgg gcgctggctc
    11641 gatttggttt ttatgcaacg tatccttttg cagacaggtg tcgcgctcgc gaagtgaaac
    11701 aaagtcgtgc atccacgcgc gcaaaagtgg aattacgaca cagatctctc gtaggacggc
    11761 ctaagccacg tttgttgcta tcgtagtctt gatatcatgc gccggtggta agagcgaccc
    11821 agactgaaat cgagcatgcc gtcgaggcat tgcgcgccgg cgaactggtg gtgttcccga
    11881 ccgagaccgt ctacgggctc ggagcgaatg ccgccaatcc cgctgccgtg cgcaagattt
    11941 tcgaggtcaa gggccgtccg cccgatcacc cggtgatcgt gcacctggat gatccgcgct
    12001 atctgcatcg ctgggtgtcg tcggtgccgc ccgtcgccga gcagctcgcc gcccgattct
    12061 ggccgggccc gttaaccctg attctgccca aggccgacaa tgtcaacgac atcgtgaccg
    12121 gcggccagga cagcatcggt atccgcgtac cctcgcatcc catggcgcag cagctcttga
    12181 ccgcgttcgg cggcggcatt gccgcgccct ccgccaatcg gtacggacga ttgagtccga
    12241 ccaaacccga gcacgtgcgc gacgaactcg gcagtgcggt caagatcatg ttggacggcg
    12301 gcgattcgcc gatcggcttg gaatcgacca tcgtctcgtg tctcgacaat caggcgcgtt
    12361 tgttgcgccc cggcttcatc acgcgctcgc agctcgagca ggccgtcggc agcctcgccg
    12421 tcggcggaat ggtcccgcgt gcgccgggcg atcgcgccct gcactacgcg ccgtcgacgc
    12481 cgctcgagat cgtgtcctcc gatgacctcg aggtgcgagc cggcgaggtc accgcccgcg
    12541 aagaaaaggt ggcggtgctc gccctgcggc cgcccctgca gactcagcgc catatgacct
    12601 ggatcaatgc cggcaagcgt ccggacgttt atgcacacaa tctttacaat catttgcgca
    12661 ccctggaccg tgccggttgc gtgaaaatct tcgtacaggg cctgccgcag gacgaacgct
    12721 gggcggcgat tctcgaccgc ctgcagcgtg ccagcggcat gggcggcgac gattctctcg
    12781 agatgagcgt cagttagtgg ccaagccgct gctcaagaca ggatcggcgc gggacgcggg
    12841 gctcgaactc gatcccgcgc gcgatccatt tgcgcccgag tcgatcaagc acttggtgag
    12901 ccgctacggc tcgccgctat tcatcatcga tgcggagcgc gtgcgccgcc aatatcgccg
    12961 gctggcggcg gcgctgccgc gagtcgacct gcactatgcc ctgaaaccgt tgccgcacgc
    13021 ctccgtcatc agcacgctcg ctgccgaagg cgcgtgcttc gatctcgcga ccaacggcga
    13081 agtggaattg gtacgccgcc tgaagatcgc accgcaacgc tgtattcaca cccaccccat
    13141 caagcgcgac agcgacatcc gcacggcgct tgcgtacggc gtcaatcgat tcgtcgtcga
    13201 caacgtcgat gagctgcgta aattcgtgaa gtttcgcacc cggtcatcgc tgctgattcg
    13261 agtctccttc agcagccccg aggcgaggtg cgatctgtcg cgaaagttcg gttgtgaacc
    13321 cgaggcggcc gtcgacctgt ttcgcctggc ggcgcagctc aacatcaaga tcgacggatt
    13381 gtcgttccat gtcggctcgc aggctgcggg gccggccatg ttcgtcgagg ccatcgcccg
    13441 ctgcgcgcag cttttggcga gcgccgggga gcgcggacac gccccgagca tcttggatat
    13501 cggcggcggc ttcccggtcg attatctcga gcgctgcatg ccgatcgagg agttctgtgc
    13561 gccgattgcg gctgctttgg ccgcgttgcc ggctggcatt cgcgtcatcg cggaaccggg
    13621 acgctatatt tcagcaccgg cggcgatcac ggccgcacag gtcatggggc gtgcgcagcg
    13681 cgacggacga tggtggtact acctcgatga cgggctgtac ggcagttaca gcggccaggt
    13741 ttacgaccat gcgcgctacc cggtcgaggc gctggtcgcc gccggtaaga cttatccgtc
    13801 ggtgctcgcg ggtccgacct gcgacagcat cgatgtcatc aacgaccact tggaactgcc
    13861 gatgctcaag atcggcgacc tcgtcatcgg ccacgcgatg ggcgcgtata cctgggcctc
    13921 ggcctcggaa ttcaacttct tcccgcgcgc cacggtgctg gcgctcgacc gcggcagact
    13981 gctcaccgat gggagctgcc catgacgtgg agccggcgcc gggtgcttct gagtggcagc
    14041 gcactcgccg gcttgtcggc gctcacttca gtggcgcgtt ccaccgcagc gcagcggccg
    14101 gcgccgaagc gcattgcgct cgcgaatttg cataccaacg aacggttgga ggtcgaatac
    14161 tgtcgcgacg ggcgctacct gcccgaggcg ctgaaggcga tcgaagcggt gttgagagac
    14221 tttcgcagcg gcgagcagca cccgatcgat ccaaagctca tggattatct gatcgacgtg
    14281 gcgcacgcgg cgggcgccga tccggcattc agcgtgatct cgggctaccg ctcgccgcaa
    14341 acgaatgcgc gcctgcgcga gacgagcacg ggtgttgcgc agcgcagttt gcacatggag
    14401 ggccgggcga tcgacgtcag aatggccggc gtcgactgcg cgcgtcttgc cgcgcacgcc
    14461 gaggagctga aacatggcgg cgtgggctat tatcgagcct cggatttcgt gcacctggat
    14521 accggagctt ttcgaacttg gaagggttga gcgcgtagcc gcgcggcgct caggtacgta
    14581 cgcggcgcgc gtcgagcgcc ttctcgagcc gtgcatcgtg cttgtagata tcgtcgaaga
    14641 acaggatgtt gcccctctcg gtggcgatcg ccgtgccata caggatgaaa acgcgaatgt
    14701 gattcttcaa gatcacggtg accggctgcg tgccgttcat cgcggcgagt atcttttcgc
    14761 gggtccattg cggagaatcg cgcagcacgt act
//
LOCUS       DASWZT010000237         4298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_21152, whole genome shotgun sequence.
ACCESSION   DASWZT010000237 DASWZT010000000
VERSION     DASWZT010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21152"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /locus_tag="VGE92_06585"
     CDS             complement(<1..1170)
                     /locus_tag="VGE92_06585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY1313522.1"
                     /translation="MRGSLRSALLLLAGALTACSFAPVYKTPDSAPAASAYPQAADWK
                     QAQPLDAENRGSWWTVFGDPTLDALEAKVGDANQDLKAGLARLQQARAATRIARASLF
                     PTLTAASSAARARASVNSPRFPPGSEPVGNNFDVEADVSYEIDVWGRVRNAVASARAS
                     QQASAADLATLNLSIHAELAADYFALRAADAEQVLLDQTVADFSRSLQLTQNLYDGGG
                     AALADVAQAQAQLESARTLAADIRLQRAQNEHAIAVLLGENATTFHAEPSPLPAQLAP
                     PPIDPGLPSSLLERRPDIAAAERRVAAANAQIGVARAAYFPVFSLAAAAGYDSTSSAN
                     WLTAPSRLWSVGPSGVLTLFDAGRHRAQTAQAKAIYDEQVADYRSAVLAAYQEVED"
     gene            complement(1167..2339)
                     /locus_tag="VGE92_06590"
     CDS             complement(1167..2339)
                     /locus_tag="VGE92_06590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY1313523.1"
                     /translation="MVEASADDDSETALRRKIRRYSWILLIVAVILAVWGEVSRVLAR
                     SDLGKQTAEAALLTVITVTPNRTSLGEELVLPGTVQAFIEAPIYARTSGYLKTWLTDI
                     GTQVSKGQLLGEIETPEVDQQLSQAQADLATARANESLSDSTNARWKGLLATESVSKQ
                     DADEKAGDAAAKKAAADSAAANLARLRELESFKRVVAPFSGVITARNTDIGALINAGQ
                     SAGSELFRIADTQRLRIYVQVPEAYAAATNPGLKAELYFAEQPHRSFEATTVRTSNAL
                     DPNLRTLQVELELNNEKREVFPGAYAEVHFKLPASAESLRLPANTVLFRSAGLQVATV
                     DNQQHVKLKGIVQGRDFGNTIEVLSGLASDDKVIVNPPDSLTDGVPVRIVSPRKTT"
     gene            complement(2332..>4298)
                     /locus_tag="VGE92_06595"
     CDS             complement(2332..>4298)
                     /locus_tag="VGE92_06595"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1313524.1"
                     /translation="IENINYHLEHGKDVETAILDGAHQIALPALVSTLSICIVFVPMF
                     LLAGIAKFLFIPLAEAVVFAMLASYILSRTLVPTLAKFWLKQHDTSAKPKRANFLARF
                     QQRFEHGFEKMRDNYHALLKIAVHSGPRFAAIFLGAMAATALLAFPLGPLPGLGQDFF
                     PSVDGGQIKLHVRARTGTRIEETAALCDAIEASIREVIPADQLSTIVDNIGLPYSGIN
                     LAYSTSAPVGPGDADIFVNLNSGHKPTADFVSALRTKLAASFPSTTFAFLPADMISQI
                     LNFGLPSPIDVQIVGFNVPANRAFANQLLRKLYSVAGAVDLHIHQSGDYPQFNIDVDR
                     TKAQLLGLTEQSVASNMLVSLSGSFQTAPSFWVDPKSGTQYQVATQTPQYRLESLNDL
                     GNTPLSGGVGGAQILSNVANVYRSSAPAVVSHYNATPSIDIYGSVQGMDLGYVSAQIN
                     KIIDGAKKDLPKGTNIVLRGQTQTMKASFNGLLFGLMGAIVLVYMLIVVNFQSLLDPF
                     IIITALPAALAGIVWMLFLTHTTVSVPALTGAIMCMGVATANSVLVVSFARERMNAGD
                     NAFEAALQAGFTRLRPVLMTALAMIIGMIPMALGLGDGGEQNAPLGRAVIGGLIFATV
                     ATLFFVPTVFSIIHGRPTSHPIHSAEISHG"
BASE COUNT          812 a         1296 c         1361 g          829 t
ORIGIN      
        1 gtcctcgact tcttgatagg ccgcgagcac cgcgcttcga tagtccgcta cttgttcgtc
       61 ataaatggct ttcgcttgcg cagtttgcgc gcgatgccgg ccggcgtcga acaaagtcag
      121 cacccccgat ggacccaccg accagagccg gctgggcgcg gtcagccaat tcgccgaact
      181 ggtactgtcg taaccggccg cggcggcgag gctgaacacc ggaaaatacg ctgcgcgtgc
      241 cacgccgatt tgcgcgttgg cggctgcgac gcggcgttcg gcagccgcaa tgtcgggccg
      301 ccgttcgagc agtgaagacg gcaggcctgg atcgatggga gggggtgcca attgcgcggg
      361 gagcgggctc ggctcggcgt gaaaggtagt ggcattctcg ccgagcaaga ccgcgatcgc
      421 atgctcgttc tgcgcgcgtt gcagccggat atccgctgcc agcgtgcggg cgctctccaa
      481 ttgcgcttgc gcttgggcga cgtccgccag cgccgcgccc ccgccgtcat acagattctg
      541 cgtcaattgc aacgagcggc tgaagtcggc gacggtctga tcgaggagca cttgctcggc
      601 gtcggccgcg cgcagcgcga aatagtccgc cgcgagttcg gcgtggatcg acaaattgag
      661 cgtggcaaga tccgcagcac ttgcttgctg gctggccctg gcggaggcga ccgcgttacg
      721 cactcgaccc cacacatcga tctcatagga tacatcggct tccacgtcga aattgttgcc
      781 caccggttcg gagccgggcg gaaatctcgg cgaattgacc gaagcacgcg cacgggccgc
      841 agaagacgcc gccgtcagcg tcgggaagag cgacgcgcgg gcaattcgcg tcgcggctcg
      901 ggcctgctgc agtcgtgcca ggccggcctt caggtcttgg ttggcatcgc cgacttttgc
      961 ttcaagcgcg tcgagcgtcg gatcgccgaa aaccgtccac cacgagccgc gattttccgc
     1021 atcgaggggc tgcgcctgct tccaatcggc ggcttgcgga taagcgctgg cggcgggcgc
     1081 actgtcgggg gtcttataga caggtgcgaa ggaacatgcc gtcaacgcgc cggcgagcag
     1141 cagcagtgcg ctgcgcagcg agcccctcat gtggtcttgc gcggagagac aatccgcacc
     1201 ggcacgccat cggtgagcga gtcgggcgga ttgacgatca ccttgtcgtc gctcgcgagg
     1261 cccgacagta cctcgatggt attgccgaaa tcgcggccct gaacgatgcc tttgagcttc
     1321 acgtgctgct gattgtccac cgtcgcgacc tgtaatccgg cggatcgaaa caggacggta
     1381 ttggccggca ggcgcagact ctcagcgctc gccggcagtt tgaagtgcac ttccgcgtag
     1441 gcgcccggaa agacctctcg cttctcgttg ttcaattcca attcgacttg caaggttctt
     1501 aggttcggat ccagcgcgtt cgatgtgcgg accgtggttg cctcgaagga tctgtgcggc
     1561 tgctcggcga agtacagttc ggctttgagt cccggattgg tcgcggcggc ataggcctcg
     1621 ggtacttgca catagatgcg cagcctctgc gtgtcggcaa tgcggaataa ttcgcttccc
     1681 gcgctctggc cggcgttgat caatgcaccg atatcggtat tgcgcgcagt aattaccccg
     1741 ctgaagggcg cgaccacccg cttgaaagac tcgagttcac gcaggcgtgc gaggttggcc
     1801 gccgccgagt cggccgccgc cttcttcgcc gccgcgtcgc ctgctttctc atcagcgtcc
     1861 tgtttggaga ccgattcggt ggcgagcaga cctttccagc gcgcattggt gctgtcggag
     1921 agcgattcgt tggcacgcgc agtcgcgaga tccgcctgag cttgactcag ttgctggtcg
     1981 acctccggcg tttcaatctc tcccagcaac tgacctttgc ttacctgcgt tccgatatcg
     2041 gtcagccaag tcttgaggta gccgctggtg cgtgcgtaga tcggcgcctc gataaatgct
     2101 tgcacggtac cgggaagcac cagttcctcg ccgaggctgg tgcgattcgg cgtgaccgta
     2161 atgaccgtga ggagggcggc ttcagccgtt tgctttccca ggtcggagcg ggccaacacc
     2221 cggcttacct cgccccaaac cgccagaatc acggccacaa tcagcagaat ccacgaatag
     2281 cggcgaatct tgcggcgcaa cgccgtttcc gagtcgtcgt cggcgctggc ctcaaccatg
     2341 ggagatctcc gcagaatgaa tcgggtgcga tgtcgggcgc ccgtgaatga ttgaaaatac
     2401 cgtcggtacg aagaaaagcg tggcgaccgt ggcgaatatg aggccgccga tgaccgcccg
     2461 gcccagcggc gcattctgct cgccgccgtc gccgagaccc aacgccatcg ggatcatgcc
     2521 gatgatcatc gccagcgccg tcatcagtac cggacgcagc cgcgtgaaac ctgcctgcag
     2581 tgccgcctcg aatgcattgt ccccggcatt catgcgttcg cgcgcaaagc ttacgaccaa
     2641 cacgctgttc gcggtggcca cacccatgca catgatcgcc ccggtgagcg ccggcacact
     2701 gaccgtggta tgcgtgagga ataacatcca gacgatcccg gccaatgccg ccggcaatgc
     2761 ggtaatgatg atgaacgggt cgagcaggga ctgaaagttc accacgatca gcatgtacac
     2821 caagacgatc gcgcccatca ggccgaacag caggccgttg aacgaggctt tcatggtttg
     2881 cgtctggccg cgcaacacga tattcgttcc tttcggcaga tctttcttgg cgccatcgat
     2941 gattttgttg atttgcgcgg acacatatcc aagatccatg ccctgcaccg agccgtagat
     3001 gtcgatggac ggcgtcgcat tatagtggct gacaaccgcg ggcgcgctgc tgcgatagac
     3061 gttggcgaca ttcgacagga tctgcgcgcc gccgactccg ccgctcaacg gcgtgttgcc
     3121 gagatcgttg agactctcca gacggtattg cggagtctgg gtggcgactt ggtactgcgt
     3181 gccgctcttc ggatccaccc agaacgaggg cgcggtctgg aagctgcccg acaaggacac
     3241 cagcatgttg ctcgccaccg actgttccgt gagcccgagc aattgcgcct tggtgcgatc
     3301 gacatcgata ttgaattgcg gatagtcgcc cgactggtgg atatgcagat cgaccgcccc
     3361 cgcgacactg tacaatttgc gcaacagctg gttggcgaaa gcgcgattcg ccggcacatt
     3421 gaagccgacg atctggacat cgatgggcga gggcagccca aagttcaata tctgactgat
     3481 catgtccgcc ggcagaaacg cgaaggtcgt cgacgggaaa ctggccgcga gcttggtgcg
     3541 cagcgcactg acgaaatccg cggtcggctt atgcccgctg ttgagattga cgaagatgtc
     3601 ggcatcgccc gggccaaccg gggcagaggt gctgtacgcg agattgatgc cgctgtacgg
     3661 caaaccgatg ttgtcgacga tggtcgataa ctgatccgcc gggatcacct cgcggatcga
     3721 tgcttcgatc gcgtcgcaaa gtgccgcggt ttcctcgatg cgggtgccgg tacgcgcacg
     3781 aacgtgcaat ttgatctgac cgccgtcgac ggacgggaag aaatcctggc caagaccggg
     3841 caggggtccg agcgggaagg cgagcagggc ggttgccgcc atggcgccga gaaaaatcgc
     3901 cgcgaaccgc ggtccgctgt gaaccgcgat tttgagcagc gcgtgataat tatcgcgcat
     3961 tttctcgaag ccgtgttcga agcgctgttg aaatcgggcc aggaagttgg cgcgcttggg
     4021 cttcgcgctc gtgtcgtgtt gcttgagcca gaattttgcg agcgtcggca ccagcgtacg
     4081 cgacaggata tacgaggcca gcatcgcgaa tacgaccgct tcagccagcg gaatgaacag
     4141 gaatttggcg attcccgcca acagaaacat gggtacgaag acgatgcaaa tcgacaaggt
     4201 cgataccaac gccggcagcg caatctggtg cgcgccatcg agaatcgccg tttccacgtc
     4261 cttgccgtgt tccaggtgat aattgatgtt ctcgatgg
//
LOCUS       DASWZT010000238         8630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_6504, whole genome shotgun sequence.
ACCESSION   DASWZT010000238 DASWZT010000000
VERSION     DASWZT010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8630
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6504"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="VGE92_06600"
     CDS             <1..1160
                     /locus_tag="VGE92_06600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEY1313525.1"
                     /translation="VLVFRDPEVVCATVLGRDPLRLAEAYFRGAMDIEGDFFAAIELK
                     KYLQALQLSVAEQIAAAAAALRLRALNAQRSSAAVERTLARRHSIKAHSREENREAIH
                     FHYDVSNEFYALWLDRAMVYSCAYFETPETDLDAAQEAKLEHICRKLSLKAGENFLDV
                     GCGWGALVIHAARHYGVRAHGVTLSAQQLQVARKRIAQAGLADRVRVDLLDYRDLTGE
                     SIYDKVASVGMFEHVGLKNLPVYFSTINRVLKPHGLFLNHGITHDSDGWQKTLATEFM
                     NRYVFPDAQLDNISNIQRAMERAHFEIADVEALRPHYALTLRHWVAGLERNHARALQH
                     VDEATYRVWRLYMAACALEFESGRVGVYQVLAGKRGSGGVPLPLTRRYLYR"
     gene            complement(1165..1500)
                     /locus_tag="VGE92_06605"
     CDS             complement(1165..1500)
                     /locus_tag="VGE92_06605"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEY1313526.1"
                     /translation="MREVTTTCTGIDQPPFFWLQGLASSNALLLTVAVLIRQNRMAQV
                     AEHRSHLDLQINLLSEQKVTKILQIVDELQRNLTALQPRPDSEVAEMTKPADAHALMH
                     AIKQKHSDP"
     gene            complement(1530..2801)
                     /locus_tag="VGE92_06610"
     CDS             complement(1530..2801)
                     /locus_tag="VGE92_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEY1313527.1"
                     /translation="MPFRNLLSLPHWKSRLLLWGAAAAVGLAAVLFAGLADAAQNALR
                     RLLEDSRFWPWLLAPAGFFLIAWITQRYFRGAEGSGIPQTIFALRPDAGAQGEAFLKP
                     KVVIGRVALAALGLLCGGSIGREGPTVHVGAVIVHGVARWMPHGRIEAQRRALVLAGG
                     AAGVAAAFNTPLAGIVFAIEELSRSFEERASGTTLTAVILAGVVAIALVGDYSYFGQP
                     VVPSQLRAISPAIIALAAACGLAGGGFSRLTLACAGSLPGILGKLRITRPAVFAGLCG
                     LAIAAIGTLSGDLTYGTGYAEARSVLESQAHLPWFYAPARALATLLSYLSGIPAGLLA
                     PSLSVGAGLGQLVADACGQPSAVPFAILGMCGYLAGVTQAPLTSLVIVMEMTAQHAMV
                     LPLMVSAALATAVSKLITPPLYQSLADRYSN"
     gene            complement(2830..3324)
                     /locus_tag="VGE92_06615"
     CDS             complement(2830..3324)
                     /locus_tag="VGE92_06615"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY1313528.1"
                     /translation="MSGDAARVSVTVAVPPPLAFEIFTAEIDRWWRRGIKFRHAGKRG
                     GFLCIEPFLGGRLFESIDGDAGPHVLEVGRVRVWQPPAHLCFTWRNANFAEHETTEVD
                     VAFAATTSGTLVTVTHRGLSALRPEHPARHGLQGAQFSRMIGLWWGEQMTSLRELCAQ
                     RITT"
     gene            complement(3321..3683)
                     /locus_tag="VGE92_06620"
     CDS             complement(3321..3683)
                     /locus_tag="VGE92_06620"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY1313529.1"
                     /translation="MEQRHCKPDLDAAFGALSDSSRREMIRTLLQQPRRAGELADSLD
                     MSPQALSRHLRVLRRAGLVVEEGIEDDARVRVYSVHPGALQPVQQWLAQIEELWRGQL
                     QAFKAYAETAQRRRRTKP"
     gene            complement(3696..4151)
                     /locus_tag="VGE92_06625"
     CDS             complement(3696..4151)
                     /locus_tag="VGE92_06625"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY1313530.1"
                     /translation="MNPTPSDWPRMSSSIFYRDAAAAIDWLCDAFGFEVRLKVEGDNG
                     RIEHSELTYGAGLIMVGQEGADPARGWRNSMRSPKSLHGACTQSIMFFVDDAAAHCAH
                     ARARGATIVEEPATHDYGAEYWADMSYGALDPEGHMWWITQRLRNPPAR"
     gene            5143..6570
                     /locus_tag="VGE92_06630"
     CDS             5143..6570
                     /locus_tag="VGE92_06630"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEY1313531.1"
                     /translation="MTTKFGELMIEKRSIDYVPIAERHGKVWHLWPVWFSGDAHLATV
                     AAGVVGIALGGTLVWMSLAVFFGCAFGTFFMAFHSTQGPQLGLPQMIQSRPQFGYVGA
                     LLVWGVALIAYIGFNVFNQILAAQTVLSLYGGDSTWVMLVFTLLSVALAVIGYDLIHL
                     AQRSVAYLLIAALLVFTVAVVAKGNLGSQALALRDFRAVPFLAQFFAAASYQIAWSIY
                     VSDYSRYLPRDVGVGASFWWTYLGAFVGGTWMMLLGTLSAALYPTMEVATALRTVADS
                     VLPGFGKPLLLCSLVGLVTITSLNYYGASLTFLSVMDSIKPSRPTVGKRLATLFISAL
                     LATALALSSSHNFVEQFSEFLDVLLYLFTPWTAINLVDFYWIRKGHYSVREIFNPRGM
                     YGRWNARGLWAYGIGFVAMIPFFSTSHFKGPVARALGGSDIAMLVGLPVSALVYIVLC
                     RTLDLAADRQQAHDADRGLDPDAAY"
     gene            complement(6567..7565)
                     /locus_tag="VGE92_06635"
     CDS             complement(6567..7565)
                     /locus_tag="VGE92_06635"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY1313532.1"
                     /translation="MVSSRNKILTGALALLLLHHASAFPAAAARSAGAPPAADAAAAP
                     AAPPALKIDLDAFDNSKKLLVLPNGQSLAYIDRGERSGPAVVLIHGYTDNARDWVPLL
                     PYLSKRYRLILVDIRGHGKSSKPECCYSRLDFAYDIKLLLDSLGVRKADIVGHSLGSI
                     IAQTFAEFWPERTAHVVLISSTGGAPPDGAKKPPQFDFAAEIRKLKEPIEPDSPFMIA
                     WWDSPTPVDPDFIRRQRIDAAGIPLRVWIAVLDQALPANNIYGDLQSTLPRLKAPTLL
                     IWGSRDPIMEEDVRQTLRRALPNAQVKIFDGLGHNPFWEDPQGVAKVLNAFLDTPL"
     gene            complement(7597..8385)
                     /locus_tag="VGE92_06640"
     CDS             complement(7597..8385)
                     /locus_tag="VGE92_06640"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1313533.1"
                     /translation="MSRSASHFAFNRHGVWAIYKFEIARAMRTLVQSLVTPVITTSLY
                     FVVFGAAIGSRLTTPGGVSYGAFIVPGLIMLALFTESLSNASFGIYFPKFTGTIYELL
                     SAPVSYVEIVIAYVGAASTKSVVLGLTILLTASLFVPIKILHPIWMTAFLLATAFTFS
                     LFGFIIGIWAKGFEQLQFIPMLVITPLTFLGGAFYSIDMLPPAWRTFSLFNPIVYLIS
                     GFRWSFYGTADVSVGISLAMTLGFLGICVAVVAWVFKTGYRLKS"
     gene            complement(8382..>8630)
                     /locus_tag="VGE92_06645"
     CDS             complement(8382..>8630)
                     /locus_tag="VGE92_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313534.1"
                     /translation="GKKQLILHLQEPLQRIPLELKSWALELKSDGHDLVYTVDSANSG
                     NILALLRRLSDMSILVKDLQTHQSSLEEIFVKLVSEPA"
BASE COUNT         1620 a         2593 c         2771 g         1646 t
ORIGIN      
        1 tcgttttggt gttccgcgat cccgaggtgg tctgcgccac ggtgctcggt cgcgatccgt
       61 tgcggctcgc cgaagcctat tttcgcggcg cgatggatat cgagggcgac ttcttcgccg
      121 ctatcgagct gaagaaatac ctgcaggccc tgcagctgtc cgtggcagag cagatcgccg
      181 cggccgccgc ggcgctgcgt ttgcgcgcat tgaatgcgca gcgcagctcc gccgcagtcg
      241 agcgcacgct cgcgcgccga cacagcatca aggcccactc ccgcgaggaa aatcgcgaag
      301 cgattcattt tcactacgac gtttcgaacg agttttatgc gctgtggctc gatcgcgcca
      361 tggtctattc ctgcgcctat ttcgagacgc ccgagaccga tctggatgcg gcgcaagaag
      421 cgaagctcga gcatatttgc cgcaagctgt ccttgaaggc aggggagaat tttctcgatg
      481 tgggatgcgg gtggggcgcg ctcgtgatcc atgcggcacg gcattacggc gtgcgcgctc
      541 acggggtgac cttgagtgcg cagcagttgc aggtggcccg caagcgcatt gcccaggcgg
      601 gcctcgcgga ccgcgtgcgg gtcgatttgc tggattaccg ggatctgacg ggcgagtcga
      661 tctacgacaa ggtggcgagc gtcggcatgt tcgagcatgt cggcctgaag aacctgccgg
      721 tgtatttttc cacgatcaac cgagtcctca aaccgcacgg cttgttcttg aaccacggga
      781 tcacgcacga ctcggacggc tggcaaaaaa ccctggcgac ggaattcatg aaccgttacg
      841 tgtttcccga cgcgcagctc gacaacatca gcaatattca gcgcgcgatg gagagggcgc
      901 actttgagat tgccgacgtc gaggcgctgc gtccgcacta cgcgttgacg ctgcgccact
      961 gggtcgccgg attggaacgc aatcacgccc gggcgttgca gcatgtcgat gaggcgacat
     1021 accgtgtgtg gcggctgtac atggccgcct gtgcgttgga gttcgaatcg gggcgcgtcg
     1081 gcgtctatca ggtgctggcg ggcaaacgcg gcagcggcgg cgtgccgctg ccgctcacgc
     1141 ggcgctatct gtaccgctga acgttcacgg atcgctgtgc ttttgtttga tcgcatgcat
     1201 cagcgcatgc gcgtccgcgg gcttcgtcat ctcggccact tccgaatccg gccgcggctg
     1261 cagcgccgtc aggttgcgct gcaactcatc gacgatttgc aggatcttgg tcactttctg
     1321 ctcgctcaga aggttgattt gcaggtcgag gtgcgagcga tgctccgcca cctgagccat
     1381 gcggttttga cgaatcagca ccgcgacggt cagcagcagg gcgttggaac tcgccaatcc
     1441 ctgcagccaa aaaaacggcg gttggtcgat gcctgtgcat gtcgttgtga cctcccgcaa
     1501 ccgataaagg ttcgcgcgga ttgcggtctt tagttcgagt agcggtctgc cagcgactgg
     1561 tacaggggcg gggtgatcag cttcgatacc gcggtggcga gggcggcact gaccatcagc
     1621 ggtagcacca tggcgtgctg cgcggtcatt tccatgacga tgaccagtga ggtgaggggc
     1681 gcctgggtca cgccggcgag gtagccgcac ataccgagga tggcaaacgg caccgccgat
     1741 ggttggccgc aggcatcggc aaccaactgt cccaagcccg cacccaccga cagggacggc
     1801 gccaaaagcc cggcaggaat gccgctcagg tacgacagca gggtcgcaag cgcccgcgcc
     1861 ggtgcgtaga accacggcag gtgagcctga ctttcgagaa cggaacgtgc ctcggcgtac
     1921 cctgtgccgt aggtgaggtc accgctcagc gtgccgatcg ctgcaatcgc caatccgcac
     1981 agtccggcaa atacggccgg acgcgtgata cgcaatttac ccaagatacc cggcagcgag
     2041 cctgcgcagg caagcgtcag gcggctgaag ccgccgcccg cgagaccgca cgcggccgcg
     2101 agcgctatga tcgcgggtga gatcgcacgc agttgcgacg gcaccaccgg ctggccgaag
     2161 tagctgtagt cgcccaccag ggcaatggca acgacgccgg cgagaatgac cgccgtcagc
     2221 gtcgtgccgc tggcgcgttc ttcgaacgaa cgtgacaatt cttcaatggc aaatacgata
     2281 ccggccaacg gcgtattgaa cgccgcggcc accccggccg cgccgcccgc gagtaccagc
     2341 gcgcgacgtt gggcttcgat gcgcccgtgc ggcatccatc gcgccacgcc atgcacgatg
     2401 acggcgccga catggacggt gggtccttcg cggccgatcg agccgccgca cagcagtccc
     2461 agggcggcca atgcgacgcg tccgatcacc actttgggtt tgaggaatgc ctccccttgc
     2521 gccccggcat ccggccgcaa agcgaaaatc gtctgcggga tgccgctgcc ttcggcgcca
     2581 cgaaagtagc gttgcgttat ccaggcgatc aagaaaaaac cggccggcgc gagcagccac
     2641 ggccagaagc gcgagtcctc aagcaatcgt cgcagcgcgt tttgcgcggc atcggcaagg
     2701 ccggcaaaca gcacggcggc gaggccaacc gctgcggccg cgccccacag cagcaaccgg
     2761 gacttccagt gcggcagcga taagaggttt cgaaatggca tcgacttcaa tatagcagcg
     2821 cacgcacagt caggtggtga tgcgctgcgc gcacagttcg cgcagcgaag tcatctgttc
     2881 cccccaccac agtcctatca tgcggctgaa ttgcgcgcct tgcaggccgt gccgcgccgg
     2941 atgctccggt ctcaaggcgg atagaccgcg gtgcgtcacc gtgaccagag tccctgaggt
     3001 cgtagccgcg aaggcgacat ccacttcggt ggtttcgtgt tcggcaaaat tggcgttgcg
     3061 ccaggtgaag caaagatgcg cgggcggctg ccagacgcgc acgcgaccga cttcgaggac
     3121 atgcggtccg gcatcgccat cgatcgattc aaacagacga ccgccgagga agggttcgat
     3181 gcacaaaaac ccgccgcgct tgccggcgtg tcggaacttg atgccgcgcc gccaccagcg
     3241 atcgatctcg gccgtgaaaa tctcgaaggc cagcggcggc ggcaccgcta ccgtgacgct
     3301 gacccgggcg gcgtcgccgc tcatggcttg gtgcggcggc ggcgctgcgc tgtttcggca
     3361 tacgctttga acgcctgcaa ttggccgcgc cacagttctt cgatctgtgc cagccattgc
     3421 tgcaccggct gcaaggcgcc cggatgcacg ctgtagacgc gaactcgcgc atcgtcctcg
     3481 atgccttctt cgaccaccag gcccgccctg cgcaagacgc gaagatgccg gctgagtgct
     3541 tgcgggctca tgtcgaggct atccgccagt tcacccgcac ggcgcggctg ctgcagcaga
     3601 gtgcggatca tttcgcggcg cgaagaatcg gacagcgcgc cgaaggccgc atccagatcg
     3661 ggtttgcagt ggcgttgctc cacgggaaat ccgcgttacc gcgccggtgg attgcgcagc
     3721 cgctgggtaa tccaccacat gtggccttcc gggtcgagag caccgtagct catgtcggcc
     3781 caatactcgg caccatagtc gtgagtcgcc ggctcctcga cgatggtcgc accgcgagcg
     3841 cgggcgtgag cgcagtgcgc ggcagcatcg tcgacgaaaa acatgatgct ctgggtgcag
     3901 gcgccgtgca aggacttggg gctgcgcatc gaattgcgcc agccgcgtgc gggatcagcg
     3961 ccttcctggc cgaccatgat gagccccgcg ccgtaggtca gttcgctgtg ctcgatgcgg
     4021 ccgttatcgc cttcgacctt gagccgcact tcgaagccaa aggcatcgca cagccaatcg
     4081 atggcggccg ccgcatcccg gtagaagatg ctgctcgaca ttctcggcca gtcgctcggg
     4141 gtcggattca tgcgcgtact ccaggcggtt aaatattaac gagtcatgat aatattaacg
     4201 aattggttaa agaatggcaa cgggcggcgg cggcgctctg atgatagtat tttcggatgg
     4261 tgcgtcgtga cgtaatggta cttcgatctg catatctgac agagatgtcc attgcttgct
     4321 gcgtttctcc aaggggtgga agtcccgaac cggtaagcat cggtgcgccg ggcagcaggc
     4381 cccaggtagc gacgggagac cgtcgtaccc gagcggggta tcaaaagccg gtgagttggc
     4441 gcgagccaac aagcgggggg cgaacacgcg ggccgcaact taaagtgaag cctgcagcct
     4501 cgacatattc acaacccgca ggccgagccg ctcatttcac ggcgaaggca acatcacagg
     4561 cgagagaacc gaagctcgcg tgcgactgcg gcggggtatg gggcgcagca cgcgtgtcag
     4621 gagaagagcg gaacacgaga gacccgtctg tgcggccatt gtcgtggcaa ggtgactcat
     4681 ataagccgaa ggcgaagtcg agcactgtgc agcgggagtc cgaggggatc gtagtactgc
     4741 atgacccgga gcgattcggg ggacgaacgc catgacgaac aacgtggcgg gagggatgag
     4801 gggatggggt cagtactttc gcaccggcaa tgcggcgact cgttttatcc agctcgattc
     4861 ctacgtctgg cggcggttga tgtccttgcg ccgcaagcgc aagggtcgcg acctgcaacc
     4921 cggcgaggtc atgcgctgga cacgcgagtc ctttgaaggc ctggggttat atcgcctacg
     4981 cgggaccatc cgttacccgg catccgcatt ttgggaatcg cataatgctg tcgcttaatc
     5041 gatcactggt aagccgtgtg cgggaaattc gcacgcacgg tgtggtaggg ggccctgatt
     5101 cctcctgttg cctttcggct tcctaggagt cagggtctac caatgacaac aaaattcggc
     5161 gaattgatga tcgaaaaacg atccatcgac tacgtgccga tcgccgaacg gcacggcaag
     5221 gtttggcatc tgtggccggt ctggttctcc ggagatgcgc atctggcgac ggtcgctgcc
     5281 ggagtggtcg gcatcgccct cggcggaaca ctcgtttgga tgtcgttggc ggtgtttttc
     5341 ggttgtgcgt tcggcacctt cttcatggcc ttccactcga cccaaggtcc gcaactcggg
     5401 ctgccgcaaa tgatccaatc gcggccgcag ttcggctacg tcggggcgct gctggtatgg
     5461 ggcgtcgccc tgatcgccta catcggattc aacgtcttca accagatatt ggcggcgcag
     5521 accgtgctgt ccctgtacgg cggcgattcg acgtgggtga tgctcgtttt cacgctgttg
     5581 tccgtggccc tcgccgtcat cggttacgat ttgatccatc tcgcgcagcg cagcgtcgcc
     5641 tatttgctga ttgccgccct gctggtgttc acggtggccg ttgtcgccaa agggaacttg
     5701 ggctcgcaag cgctcgcatt gcgcgatttt cgcgccgtgc cgttcctggc gcagttcttc
     5761 gcggccgcct cgtatcagat tgcctggtcg atctacgtgt cggactattc gcgctacttg
     5821 ccgcgcgatg tcggggtcgg cgcgtccttt tggtggacct acctcggcgc cttcgtcggc
     5881 ggcacctgga tgatgttgct tggcacgtta tcggcggcgt tatacccgac catggaagtg
     5941 gcgacggcgc tgcgcacggt cgccgactcg gtattgccgg gcttcggtaa accgttgttg
     6001 ctgtgttcgc tggtcgggtt ggttaccatt acatcgctca actattacgg cgcgtcgctc
     6061 acgttcctga gcgtcatgga ttccatcaaa ccgagccggc cgacggttgg caagcgcctg
     6121 gcgaccttgt tcatctcggc gctgctcgcc accgccctcg cgctttcctc gtcccacaat
     6181 ttcgtcgagc aattcagcga gtttctcgat gtgctcctct atctgttcac gccttggacc
     6241 gcaatcaacc tggttgattt ctattggatc agaaaagggc actactcggt gcgcgagata
     6301 ttcaatccgc gcggcatgta cggacgctgg aatgcccgcg gcttgtgggc gtatgggatt
     6361 ggcttcgtcg cgatgatccc gttcttcagc accagccact tcaaggggcc ggtggcgagg
     6421 gcgctcggcg ggtcggatat agccatgttg gtcggactgc cggtctcggc gctggtttac
     6481 atcgtcctgt gccgcacgct cgatcttgcg gcagaccggc agcaggccca cgacgcggac
     6541 cgcggtctcg atccggatgc ggcctattag agcggcgtgt cgaggaacgc gttgagcacc
     6601 ttggcgaccc cctgcgggtc ttcccaaaac ggattgtggc cgagtccgtc gaatattttc
     6661 acttgcgcat tgggcaaggc gcggcgcagc gtctggcgca catcctcttc catgatggga
     6721 tcgcggctcc cccagatgag caaagtcggc gccttgagcc gcggcagcgt gctttgcaaa
     6781 tcgccgtaga tgttgtttgc cggcaacgct tgatcgagga ccgcgatcca aacacgcagc
     6841 ggaatgccgg ccgcatcgat gcgctggcgg cgaatgaaat cgggatccac cggcgtcggt
     6901 gaatcccacc aggcgatcat gaacggcgaa tccggctcga tcggttcctt gagcttgcgt
     6961 atctcggcgg cgaagtcgaa ttgcggcggc tttttcgcac cgtcgggggg cgcgccgccg
     7021 gtcgaggaga tcagcaccac atgcgcggtc cgttcaggcc aaaattccgc gaatgtttgc
     7081 gcgatgatgc tgcccagaga gtgtccgacg atatccgcct tgcgcacgcc gaggctgtcc
     7141 agcagcaact tgatgtcgta ggcgaaatcc aagcggctgt agcaacactc gggtttgctc
     7201 gatttcccat ggccgcgaat atccaccagg atcagccggt agcgcttcga tagatagggc
     7261 agcagcggca cccaatcgcg cgcattgtcg gtgtaaccgt ggatcagcac gacggcgggg
     7321 ccggagcgct cgccccgatc gatataggcg agcgactgcc cgttcggcag cacgagcaat
     7381 ttcttactgt tgtcgaaggc gtcgagatcg attttcagtg cgggcggcgc tgccggcgcc
     7441 gctgcggcgt cggcagcagg tggcgcgccg gcggagcggg ccgcggctgc cggaaatgcc
     7501 gaggcatggt gcagcaacag caacgcgagt gcgccggtca atatcttgtt tcttgagcta
     7561 accatgatcg aagcttaccc gaggacgccg ctgccgctag ctcttcaaac gatagcctgt
     7621 cttgaacacc cacgcgacca ccgccacgca gatgcccaag aagcccagcg tcatcgcgag
     7681 gctgataccg acgctcacat cggcggttcc atagaagctc cagcgaaagc cgctaatcag
     7741 gtacacgatg ggattgaaga gactgaaggt gcgccacgcg ggcggcagca tgtcgatcga
     7801 gtagaacgcg ccgcccaaga aggtgagggg ggtgatgacg agcatcggaa tgaactgtaa
     7861 ttgctcgaag cccttggccc atatgccgat gatgaagcca aacaagctga acgtgaatgc
     7921 ggtcgcgagc aggaaggcgg tcatccaaat cggatgcaag atcttgatgg gcacgaacag
     7981 cgatgcggtg aggagaatgg tcaaaccgag caccactgac ttggtcgatg cggcgcccac
     8041 atacgcgatc acgatctcga cgtaagagac cggcgcggac agcaactcat agatcgtgcc
     8101 cgtgaatttc gggaaataaa tgccgaagga ggcgttcgac aggctttcgg tgaacagtgc
     8161 cagcatgatc agtcccggca cgatgaacgc gccgtagctg acgccgcccg gcgtcgtcag
     8221 ccgcgagccg atggccgcgc cgaacacgac gaagtacaag gaggtcgtga tcacgggcgt
     8281 taccaggctc tgtaccaagg tgcgcatcgc gcgcgcgatc tcgaacttgt agatcgccca
     8341 cacgccgtgc cgattgaatg cgaaatgcga ggcggatcgg ctcatgcggg ttcgctcacc
     8401 agcttgacga atatctcctc cagggagctt tgatgcgttt gcaggtcctt gacgaggatc
     8461 gacatgtcgc tgagccgcct gagcaatgcg aggatgttgc cgctattcgc gctatcgacc
     8521 gtgtagacca ggtcatggcc atcgcttttc agttcgagcg cccaggattt gagttcgagc
     8581 gggatgcgtt gcagcggctc ctgcagatgt aaaatgagtt gcttcttgcc
//
LOCUS       DASWZT010000239         2577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_26022, whole genome shotgun sequence.
ACCESSION   DASWZT010000239 DASWZT010000000
VERSION     DASWZT010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26022"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2577
                     /locus_tag="VGE92_06650"
     CDS             <1..>2577
                     /locus_tag="VGE92_06650"
                     /inference="COORDINATES: protein motif:HMM:NF017930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313535.1"
                     /translation="RYSFARHATPRTHLLSNGDYSVMLTAAGSGYSRYRNIAVTRWRE
                     DATRDAWGSYVFLRDVATGQVWSAGYQPAGIEPDSYEVAFFEDRAEIVRVDGSLTTCT
                     EILLSSEENAEVRRVSLSNDGKRVREIELTTYAEVALAAPDADAAHPAFSKLFVQTEF
                     VAASGALLATRRRRDPADPELWVSLVSALEGDGIGALQVETDRAQFLGRGRGLENAQS
                     IVEAQPLSGTVGTVLDPILSMRRRVRIAPGETTRVAFWTSVSASRAAAVGLAEKQRDA
                     GAFERAKTLAWTQAQVQLRYLGVDFDEAQTFQRVANRVLFSDSALRAPRAILDKNHLG
                     QSALWPLGISGDVPIVLVRIDDEHDLQIIKELLRAHEYWRLKQLAVDLVILNDMPPSY
                     ASLLQQSIDSAIRASRSTSDSERGRGSVFSLRQEALSEAVLILLQVTARAVFVARRGT
                     LSEQITKVREPAPLPRPPARSKQRGARSEATRAPPALEFFNGVGGFSDAAREYVIVLD
                     EEQWTPAPWSNVIANARFGFMATADGIGSTWSLNAQQNQLTPWSNDPVGNPPSEVIYL
                     RDDDGGDVWSVTPLPIRQPGHSYVIRHGFGYSQFEHESRGIAAELKQFVPLTDSIKIA
                     RLELVNRSSLVRQLTVTHYVDWVLGNQRSKTAPFIVTEIEPRTRALLVRNPWSAAEYQ
                     TQVIFLDLGGQQQSCSADRLEFLGSHGSLSEPAALSSSSALSDRVGAGLDPCGAMQTR
                     ITLRPNEARNLLLLLGAAPSAGDAVALIERYRSIDLDGVLKDVTDFWDETLGAIQVKT
                     PDRSMDLLLNGWLLYQTLACRVWARAAFFQSSGAYGFRDQLQDVMALTVSAPAIARAH
                     LIR"
BASE COUNT          460 a          805 c          830 g          482 t
ORIGIN      
        1 gcgctacagc ttcgcccgcc atgccacgcc gcgcacccat ctgctatcga acggtgatta
       61 ttcggtcatg ctgaccgccg ccgggtcagg ctacagccgc taccgaaaca tcgccgtgac
      121 gcgctggcga gaagatgcga cccgcgatgc gtggggcagc tacgtgttcc tgcgtgatgt
      181 cgcgaccggc caggtttggt ccgccggtta ccagccggcc ggcatcgagc ccgattcgta
      241 cgaagtcgca tttttcgaag accgcgcgga gatcgtgcgc gtcgacggat cgctcaccac
      301 gtgcacggag atattgctgt cttcggagga aaatgccgaa gtgcgccgcg tgtcgctcag
      361 caacgacggc aagcgcgttc gggagatcga gctcaccacg tatgcagaag ttgcgttggc
      421 ggcgcccgac gcggacgcgg ctcacccggc gttcagcaaa ctgttcgtgc aaaccgaatt
      481 cgtcgccgcc agcggggcat tgctcgcgac gcgccggcgc cgggatcccg ccgatcccga
      541 gctttgggtg tcgctggtga gcgcgttgga gggagacggc atcggcgcct tgcaggtaga
      601 aaccgaccgc gcccagttcc tcggccgtgg gcgcggcctc gagaatgccc agtcgatcgt
      661 cgaggcgcaa ccgctctccg gcaccgtggg aaccgttctc gatccgatcc tgtcgatgcg
      721 ccgtcgtgtc cggatcgcgc ccggagagac gacgcgcgtg gcgttttgga ccagcgtcag
      781 tgccagccgc gccgcggccg ttggattggc ggagaagcag cgcgacgcgg gtgctttcga
      841 gcgggccaag accctcgcgt ggacgcaggc acaggtccag ctgcgctatt tgggcgtgga
      901 ttttgacgaa gcgcagacct ttcagcgggt cgccaatcgg gtgctgtttt cggattctgc
      961 tctgcgcgcg ccgcgcgcca ttctcgacaa gaaccacttg gggcagtctg cgttatggcc
     1021 gctcggcata tcgggcgatg tgccgatcgt gctcgtcaga atcgacgacg aacacgacct
     1081 gcaaatcatc aaggaactgc tgcgtgccca tgaatactgg cgcctgaaac aactcgcggt
     1141 ggatctcgtg atcttgaacg acatgccgcc ctcgtacgct tcgctgttgc agcagtcgat
     1201 cgattccgcc attcgcgcat cgcgctcgac ctccgacagc gagcgaggcc gaggcagcgt
     1261 gttttctctg cgccaggagg cgctgtcgga ggcggtgctg attctgctgc aggtcactgc
     1321 gcgcgccgtg ttcgtggcgc gacgcggcac gttgagcgaa cagatcacca aagtgcgcga
     1381 gccggcaccg ctcccccggc cgcccgcgcg cagcaaacag cgtggggcac ggtccgaagc
     1441 gacacgcgcg ccgccggcgc tcgaattctt caacggtgtg ggcggctttt ccgacgcggc
     1501 gcgcgagtac gtgatcgttt tggacgagga acaatggaca ccggcacctt ggagcaacgt
     1561 gattgccaat gcgcgtttcg gattcatggc caccgcggat ggaattggca gcacctggtc
     1621 gctcaacgca caacagaatc aattgacgcc ctggtcgaat gacccggtcg gcaacccccc
     1681 atcggaggtc atctacctgc gtgacgatga cggcggggat gtctggagcg tgacgccttt
     1741 gcccattcgc cagccagggc actcgtacgt gatccggcat gggttcggct acagccaatt
     1801 tgaacatgaa tcacggggca tcgccgcgga gctcaagcaa ttcgtaccgt tgacggactc
     1861 gatcaaaatt gcacgactcg aactcgtgaa tcgctcgtcg ctcgtgcgcc agctcaccgt
     1921 gacccattac gtcgattggg tgctcggcaa ccagcgcagc aagactgcgc cgttcatcgt
     1981 gactgaaatc gaaccgcgaa cccgggcact gctggtgcgc aatccctgga gcgcggcgga
     2041 atatcagacc caggtgattt tcctcgattt gggagggcag caacagagtt gcagcgccga
     2101 tcggctcgaa ttcctgggta gccatggctc gctgtcggaa cccgccgcgc tttcgagttc
     2161 gagcgccctg tcggatcgag tgggggcggg actcgacccc tgcggcgcca tgcaaactcg
     2221 aatcacctta cgcccgaacg aagcacggaa ccttttgctg ctgctcggag ccgcgcccag
     2281 tgcgggcgac gcggtcgccc tgatcgaacg ctaccgatcg atcgatctcg acggcgtcct
     2341 gaaagacgtg acggattttt gggacgagac gctcggcgca atccaggtga agacacccga
     2401 tcgatccatg gacctgttgt tgaatgggtg gctgttgtat caaacgctgg cgtgccgtgt
     2461 ctgggctcgg gcagccttct ttcaatcgag cggtgcctac ggatttcgtg atcaattgca
     2521 ggatgtcatg gcgctgaccg tgtcggcgcc ggccatcgcg cgcgcgcatt tgatccg
//
LOCUS       DASWZT010000240         4001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_74368, whole genome shotgun sequence.
ACCESSION   DASWZT010000240 DASWZT010000000
VERSION     DASWZT010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74368"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1846)
                     /locus_tag="VGE92_06655"
     CDS             complement(<1..1846)
                     /locus_tag="VGE92_06655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY1313536.1"
                     /translation="MILESNDRVLEGQPQSHAPSAAPLDPVLGGLARLAANACGSPLA
                     SIAIMGCGEVWSSATAQLTLAAVPRLDPFACYTSHAVGLFEVSDATLDNRFRESEAAL
                     GFAIRSYAGCVLSGAGGETLGTLAVFDKRARVLSTDERTSLLLIAEQVVARIADRARL
                     AELALLANVNPTAAALMESAPVAIYHTDAAGNMVYANPEYRRMFGLKLGHSPDAWAEL
                     VHPDDRRQMEQSWDAFCRHPVPVRFNYRTQAKDGAIRHLSEQVVAAEGVPGWVGTISY
                     VSDLVAARDNLRKAETLFTNTFDQAPLGIVYTDRSGRLLRFNPAFCTMLGFEADELAN
                     RALGDITGNEDLTRVAAELDRLWNGEAHFLDLEKRFKRKDGSLLWVRTTTVLVREHGA
                     TPEYSVEFLRDISARKETAAELERVNKQLMSASRQAGMAEVATNVLHNVGNILNSINI
                     SASIVIERVKQSKAPGVSRLAALLEEKGAAVDDARVPEYLKALGEQLMSDQKVALEEL
                     ASLRDNLDHIKETVAMQQSYAKLCGVTETIAVADLVEDSLRLNAGAFVRHGVTLRREF
                     NEVPPITVDKHKVLQILVNLVRNAKYACDESGRADKLLILRIERAPAGV"
     gene            2051..3862
                     /locus_tag="VGE92_06660"
     CDS             2051..3862
                     /locus_tag="VGE92_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313537.1"
                     /translation="MNSAEPAPVEGPWNPGIQSQVPADLRHLQTICRPENVANSAAFT
                     ELSGLTGFGRSDLAAFRPQRLAVHELLIRVTADFAVPDGSRVGDLGINFRQIASRLHQ
                     HYVLAEMDALTAIHERARRELCDAIDAAFAAVTAAAARPVNGTRRPRAARWFAGIARR
                     RSAAASSADRSWGPSHIAECERRAGSSATALQQIAHRTLARVMSALFATQGQTWGSRD
                     LIVTLATDLACNDYGSDCIGRALEPVLERAARSEGFVLLPRQERPVVINTKGASASGK
                     STLRLLQKKLAGDIGVRWADFALISPDIWRKYLLDYASLGAAYKYAGAFTADELQIVD
                     QKLDRYMTRKHRRGEMTHLLIDRFRFDSFAPDSDEAGSNLLTRFGAAAYLFFMITPPE
                     MLVERAWKRGLEFGRYKAVDDTLAHAVQAYSGIPDVFFTWVRRGDKRIHFEFLDNTVR
                     FGEPPRTVAFGDNDTCNILDVKGTLDIERYARININASSPQQLYPDATLLEPERNTAF
                     LRRCVDGFRAINFADRDSGRIYLRMESGKAVWADQAELQAAVADPDTRCGIMSVAGAA
                     LQGDLAQAPGGPRLLDGEQAGHARTLGQWGLYPRKPQ"
BASE COUNT          702 a         1252 c         1294 g          753 t
ORIGIN      
        1 gcaccccggc aggcgcgcgc tcgatgcgca gaatgagcag cttgtcggcc cggccggatt
       61 catcgcacgc gtacttggcg ttgcgcacca ggttcaccag tatctgcagc accttgtgct
      121 tgtcgacggt gatcggtggc acctcgttga attcgcgccg caaggtcacg ccgtggcgca
      181 cgaaggcacc ggcgttcaaa cgtaggctgt cctcgacaag atcggcgacc gcgatggtct
      241 cggtgacgcc gcataacttg gcgtagctct gctgcatcgc cacggtttct ttgatgtggt
      301 ccaggttgtc gcgcagcgag gcgagttcct cgagcgccac tttctgatcg ctcatcagtt
      361 gctcgcccag cgctttcaag tattcgggta cgcgtgcatc gtcgactgcg gcgccttttt
      421 cctcgagcaa cgcggccaac cgcgataccc cgggggcctt cgactgtttg acgcgctcga
      481 tgacaatgct cgccgaaata ttgatgctgt ttagaatgtt gccgacgttg tgcaggacgt
      541 tggtggccac ttccgccatg ccggcctggc gcgaggcgct catcagttgc ttgttcaccc
      601 gttccaattc cgcggcggtt tccttgcgcg cgctgatatc gcgcaagaac tccaccgagt
      661 actcgggtgt ggcgccatgt tcgcgcacca ggaccgtggt ggtgcgaacc cacaagaggc
      721 tgccgtcctt gcgtttgaag cgtttctcga gatccaagaa atgcgcctcg ccgttccaca
      781 ggcgatcgag ttctgcggcg acgcgcgtca gatcctcgtt gccggtgatg tcgccgaggg
      841 cgcggttcgc gagttcatcg gcctcgaaac cgagcatcgt gcagaacgcc gggttgaagc
      901 gcagcagccg gccgctgcga tcggtataga cgatgccgag aggcgcctga tcgaaggtgt
      961 tggtgaacag agtctccgct ttgcgcaaat tatcgcgggc ggcgaccaaa tcgctgacgt
     1021 agctaatcgt gccgacccag ccgggcacac cctcggccgc gaccacttgt tcggacaggt
     1081 ggcgaatcgc gccatctttg gcctgggtgc gatagttgaa tcgcaccggt acgggatgac
     1141 ggcagaaggc gtcccaagat tgttccatct gccggcgatc gtcggggtgc acgagttctg
     1201 cccaggcgtc ggggctgtgg cccaatttga gaccgaacat gcgccggtac tcaggattgg
     1261 cgtagaccat gttgccggcc gcgtcagtat gataaatcgc aaccggggcg ctctccatga
     1321 gcgcggcggc cgttggattc acattggcga gcagcgccag ctcggcgagc cgcgcgcgat
     1381 cggcgatgcg cgctaccacc tgttcggcga tcagcagcaa ggaagtgcgc tcatccgtgg
     1441 acagaacacg cgcccgcttg tcgaacacgg caagcgtgcc cagcgtctcg ccgccggcgc
     1501 cggagaggac gcagcccgcg taagagcgga tggcgaagcc caacgccgcc tccgattcgc
     1561 ggaagcgatt gtcgagggtt gcgtccgata cctcgaacag tccgactgcg tgcgaggtgt
     1621 aacacgcgaa cggatcgagg cgcggcacag cagcgagggt cagctgcgcc gtggcgctgc
     1681 tccacacttc gccgcacccc atgatggcaa tcgaggcgag cggcgagccg cacgcgtttg
     1741 ccgcaagccg cgccaagccg cccaacacgg ggtcgagtgg ggcggccgac ggcgcatggc
     1801 tctgcggctg cccttcgagt actcgatcat tcgattccaa aatcatgcgc ctacacttcc
     1861 cgcagctgtt ttctgtggct gagcgcttcc ctgccgaatc cttgggttat cggcgcctat
     1921 tggataattt gaacgtccgg cggggtggcg actggatcac aggctggaat gacggatagg
     1981 gaattacgtc gcaactttga cccttcgctc tgtaccatag cgattcgcgg ctctgggcct
     2041 aggggtaacc atgaattccg ccgaaccggc gccggttgaa gggccttgga atcccggcat
     2101 ccaatcgcag gtgcccgcgg atctgaggca tctgcagacc atttgccggc ctgaaaacgt
     2161 cgccaacagc gcggccttta ccgagctttc gggcctcacc gggtttggcc gcagcgactt
     2221 ggcggccttc cgcccgcaaa gactcgcggt gcacgagctg ctgattcggg tcaccgccga
     2281 cttcgcggtg ccggacggct cgcgcgtcgg cgatttaggc atcaattttc gtcagatcgc
     2341 gagccgcctg caccagcact atgtgcttgc ggaaatggat gccctcaccg cgatccatga
     2401 gcgcgcgcgg cgtgagctct gcgatgccat cgacgctgcc ttcgctgccg tcaccgcggc
     2461 ggcggcacgg ccggtgaacg gcacgcggcg cccgcgggcc gcgcgctggt tcgcaggaat
     2521 tgcgcggcgc cgctcggcgg ctgcgtcaag cgcggatcgc agctggggtc cttcgcacat
     2581 cgcggagtgc gagcggcgcg cgggctcgag cgcgacagcg ctgcagcaga tcgcccaccg
     2641 cacgctggcg cgagtcatgt cggccctgtt tgccacgcaa ggccaaactt ggggctcgcg
     2701 agatctcatc gtgacgctcg cgaccgattt ggcttgcaat gattacggca gcgattgcat
     2761 cggccgcgcg ctcgaacccg tgctcgagcg ggcggcgcgc agcgaaggct tcgtcctgtt
     2821 gccgcggcag gaacggccgg tggtgatcaa taccaaggga gcgtccgcgt cgggcaagag
     2881 cacgctgcgc cttttacaga agaagctggc gggcgacatc ggcgtgcgct gggcggattt
     2941 cgcgctcatc agtcccgaca tctggcgcaa gtacttgctc gactacgcct cgctcggtgc
     3001 cgcgtataaa tacgcgggcg cttttaccgc cgatgaactg caaatcgtcg atcagaaact
     3061 cgaccgctac atgacgcgca aacatcggcg cggcgagatg acgcacctgc tgatcgaccg
     3121 ctttcgcttc gacagcttcg cacccgattc ggacgaggcg ggcagcaacc tgctgacgcg
     3181 cttcggcgcc gccgcgtatc tgtttttcat gattacgccg cccgagatgt tggtcgaacg
     3241 cgcgtggaaa cgcggcctcg agttcggccg ctacaaggcg gtcgatgaca cgctggcgca
     3301 cgcggtgcag gcatacagcg gaattcccga cgtgttcttc acctgggttc gccgcggcga
     3361 caaacgtatc cacttcgaat tcctggacaa cacggtgcgc ttcggtgagc cgccgcgcac
     3421 cgtggccttc ggcgacaacg acacctgcaa catactcgat gtgaagggaa cgttggatat
     3481 cgagcgctac gcgcgcatca atatcaatgc gtcgagtccg cagcagctgt acccggatgc
     3541 cacgctgctc gaacccgagc gcaatacggc tttcctgcga cgctgcgtcg atggatttcg
     3601 cgcgatcaat tttgccgatc gcgacagcgg gcgaatctat ctgcgcatgg agtccggcaa
     3661 agccgtgtgg gcggaccagg ccgaactgca agccgcagtc gcggatccgg acacgcgctg
     3721 cggcatcatg tcggtcgcag gcgcggcctt gcagggtgat ttagcacaag cgcccggcgg
     3781 tccgcgcctg ctcgatggcg aacaggcggg tcacgcgaga acgctcgggc aatggggtct
     3841 ttacccgagg aagccgcagt agcggtcaat gtgcgtggcg agatcgagag tgtgctggcg
     3901 cgtcaagcgc tccgccaatt cggtgtcgcg ccgttccaag gcttcgatga ttttcaaatg
     3961 atcgatgatc gagcgtgcag cgcgatcgct ttgcgtaatg g
//
LOCUS       DASWZT010000241         8430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_26026, whole genome shotgun sequence.
ACCESSION   DASWZT010000241 DASWZT010000000
VERSION     DASWZT010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8430
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26026"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2944)
                     /locus_tag="VGE92_06665"
     CDS             complement(<1..2944)
                     /locus_tag="VGE92_06665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY1313538.1"
                     /translation="MADALRHIDRAWRVSLDESAELVWIYGLLLSLEGRDHDAALRLL
                     QRVKTPDADVHALLSRTLLCLDRAEEARQILESALADFCIVPGGLLEHVAGEIVEHPT
                     NRAAGWIGRAANLEFTGRLVGAEPVKILQIRLNTGAAFLQPVRTSLQYGHPSFHFKLP
                     AIAAHAILEVSSRGVALIGGGCSSTPRFGLDGRTSSQGRVVHGWVRLNWLAGEPVSLR
                     IEDEHGHSVEKRSELKPRSNHRWHFHIDLRRTPVSGNRLHISALLPNGDRQSMPGMPL
                     LLEPALRLPGGTAPRLLRWKPALTARGRTQFGLNRPRVDVIIPVYRGRDETLACITAA
                     LATAGDARVVVVDDATPDAELAAALDELELNGSITLLRNARNLGFVGSVNRALARNRR
                     HDAVLLNSDAVVFGDWLKRLQAAAYREPKIGTVTPFSNSGSIASYPRTSHEPMSIEQA
                     AQFDRLAAATHEGVGIEIPVGVGFCLYVKRACLNQVGEFDESVFGKGYGEETDFCLRA
                     RRHGWSHELAADVFVHHAGARSFGAERDALLDRSQRLLNLRHPGFDRHIADFLARDPV
                     GPLRRDLDEKRLAAFPGRFVLLVTVALSGGVARFVAERCRALRTQGFFPLVLRPSEGG
                     NSRRCELWSDALDVPNLQYDIPADLSALTTVLRGLAFEAIEIQHFLHLDARVIEAVRS
                     LGPYQAVIHDYAWICPRVTLIDGSGRYCGEPSLVTCEACVKRNGSQLPESISVADLRE
                     RSATWLREARRVLAPSADAAARIRKYVDVDVVVEPHTTMPTPLPPIPPRPSAGAVIRI
                     ALIGAIGDHKGYRVLRDCARDARARGLPLEFIVIGYTQNDGPLLKTGNVFITGRFGDG
                     EVAHLLRRERPDMIFMASVWPETWSYVLDDALAVRLPVVAFDLGAIAERLRAAGLGTL
                     LPLDAHASYINENFLRLARSTVPTRPCYMEGVRKSEDKPMQNPPAIPSLGASTSTLPD
                     ALSASVQIL"
     gene            complement(3158..4183)
                     /locus_tag="VGE92_06670"
     CDS             complement(3158..4183)
                     /locus_tag="VGE92_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313539.1"
                     /translation="MGTTITVGGGNHNVVVFGNGTVIAGNGNDTITIGGVGKVTAGNG
                     NDSIAIYSGGNVTAGNGNDTISVGGFGTVKAGSGNDKITLYSGGNVSAGNGNDTIGVG
                     GWATVKAGSGNDKITLYSGGNVTAGNGNDSITVDGQGAITVGSGQDGLTLLGSGTVHQ
                     TGLSGNDTITLGFGSDTIFEAGHATVHGVFGSASISGGTLAFTDTKSGEQIEDALHGK
                     ATLVGGNFSNEFIGGTGTTIMKGGLGPDTFIGGSGMETMTGGANHNVFEFLASEAGGH
                     HVITNFIGGQDQLYLEGYSLSYLQQHHDIRISGGNTYISLDGGKTTIELQGVTKLDSS
                     DITKHKP"
     gene            4705..>8430
                     /locus_tag="VGE92_06675"
     CDS             4705..>8430
                     /locus_tag="VGE92_06675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY1313540.1"
                     /translation="MTTLFRAYELLASSELFDAEYYVRHNPDIAALNVDPVLHYLESG
                     AGRVLNPSAHFDSAYYLEQCLRNGEVTSNPLLHYITVGASRGLKPLRDAGGAEPLAAR
                     NLPGGFAIHVDRAAVIRGADGSSRLAITGWAVAQAAVSRLSAACNDVPLGEATLGIAR
                     PDVAQAFPDHAHAVNAGFSIVADFTAPEGSAQLPLTVSLLTAGGAEHRQVLQMEIDST
                     PSAEDAAAVATRTVRARVEKHPPPMQIEIDEAMVDDAGILTVSGWAVCSLDITSVSVW
                     VDGEMIGTADFGRRREDVARARKEFPRALNSGFAYNADLTRFGAGERTVKVEAVAQSG
                     IRRHALVQVLIPEPSVPLGEPLFHFHCDSISLSTAGAVYIEGWLIGATTTESLTVMLD
                     GIDIGAASIGRERPDVGQAFPGISYARNSGFTFRRAPGSAVEPGEHHIVLHVRDAAGA
                     VREVPLPVWAAEAAPDGEKSSPLAAGEDADAIALSIDAPRISNGRAETPIISSLSING
                     WALAGAGVAEVSITIDGAPVANAQHGIRRDDVASAFPDRVDAQQSGFATLIPHWLLAK
                     GAHSVCISVRDRADHVASVQFGIEVEEIPEGDGPWSLRRKMSQAEIDLQLAVLSGLAF
                     RPWFSIVMACDGEEASLAAVRRTLRSLEDQAYGHWNVWFFARGQKALGKRWRTALLAG
                     FEALEGRLKFAAPRDALNFGRAAVCPPGSWDGALVLPLLSGDELGCDALLELALAGGQ
                     NRGCDVFYGDERCHSAVTNSVQAYFKPGWSPDLLLSTNYIGRPWCVSSAVLERAGLNV
                     GEWLRHGDYDFVLRATEAAAGVGHVPKVLCQRGPCAAESEAPERQALARALTRRKISG
                     SIKKGCRRGFYRVKPNKAKYGRVSIIIPTRASRGLIKVCLETLKAKTAYRDYEIICIE
                     NIPLEDAQWKEWLRANADKVLSTDEPFNWSRYNNRCAQQASGDFLLFLNDDIEILDKD
                     WLDTLLHAAARPEVGIVGPQLLYPDGTVQHAGLALTNVMGRARHIFRHGRDNDPGYFG
                     LALAQRNVIAVTGACMLMRREIFDALNGFDEAYSVINNDLDFCLRAWRRGLLIVYTPH
                     TRLIHHEMVSRGALGDAFDSTAFIREWQGVFALGDPYLNRNLSRDSDDLVPEREPVTA
                     ICTGYPLFARSAIRRILVLKLDHIGDCITSLPAIRKLKRHFPKARITVLTGTATKLVW
                     SMEPAVDRVLEFNFFHARSQLGKVGVSEQELNDLAEQL"
BASE COUNT         1569 a         2566 c         2674 g         1621 t
ORIGIN      
        1 gcaggatttg caccgatgcg ctcagcgcgt ccggcagcgt cgatgtcgat gctcccaagg
       61 agggaattgc cggcgggttt tgcatgggtt tatcctctga ctttcgcacg ccctccatat
      121 agcacgggcg ggtcggcact gtgctcctgg caagtcgcag aaaattttcg tttatgtaag
      181 atgcgtgcgc atcgagcggt agcagcgttc ccaagcctgc ggcgcgcagc cgctcggcga
      241 tggcacctaa gtcgaacgct accaccggca agcgcaccgc caaggcgtcg tcgagcacgt
      301 agctccatgt ttccggccat acggacgcca tgaaaatcat gtcagggcgc tcacgccgga
      361 gcagatgggc aacttcgccg tcaccgaacc gtccggtaat gaagacgttt cccgttttca
      421 acagcgggcc atcgttttgc gtgtagccga tgacgatgaa ttccagcggc aggcctcgtg
      481 cccgcgcatc gcgcgcgcag tcgcgcaaca cccgatatcc cttgtgatcg ccgatggcgc
      541 cgatgagggc aatgcgaatg acagcgccgg ccgatgggcg aggcgggatc ggcggcagcg
      601 gcgtcggcat tgtcgtatgc ggctcaacga ccacgtcgac gtcgacatat ttccggatac
      661 gggccgctgc gtcggcggaa ggagccagca cgcggcgcgc ctcacgcaac caagtcgcgc
      721 ttcgctctcg caaatcagcg acggaaatcg attcaggcag ctgcgatccg ttgcgtttca
      781 cgcaggcttc gcacgtgacg agcgagggct cgccgcagta gcggccgctg ccatcgatca
      841 gggtcacgcg cggacagatc cacgcataat catggatgac ggcctggtac ggcccgagcg
      901 agcgcaccgc ctcgatgact cgcgcatcga gatgcagaaa gtgctgaatc tcgatcgcct
      961 cgaatgcaag gccgcgcagc accgtcgtca aagcggacaa atccgcggga atgtcatact
     1021 gcaaattcgg cacgtcgagc gcatcgctcc agagctcaca gcgacgcgag ttgccgcctt
     1081 cgctcgggcg cagcaccaag gggaaaaatc cctgtgtgcg caaggcgcgg cagcgttcgg
     1141 cgacgaaccg cgccacgccg ccgctgagcg ccaccgtgac cagtaggacg aagcgccccg
     1201 ggaatgccgc caagcgcttc tcatcgagat cgcgccgcag cggcccgacg ggatcgcgcg
     1261 cgagaaagtc ggcgatatgt cgatcgaagc cgggatgacg cagattgagg agccgctggc
     1321 tgcgatcgag cagcgcatcg cgctcggcgc cgaaggagcg cgcgccggca tgatggacga
     1381 agacatcggc cgcgagttcg tgcgaccagc cgtggcggcg cgcgcgcagg caaaaatcgg
     1441 tttcctcgcc atacccttta ccgaacacgg attcatcgaa ctcgcccacc tggttgaggc
     1501 aggcacgctt gacgtacaga cagaatccga cgccgaccgg gatctcgata ccgacgccct
     1561 cgtgcgtagc ggcggcgagc cgatcgaatt gcgcggcctg ctcgatgctc atgggttcgt
     1621 gcgaggtgcg tggataactc gcgatggagc ccgagttgct gaacggtgtc accgtgccga
     1681 tcttgggttc gcggtacgcg gccgcttgca gccgcttgag ccaatcgccg aaaaccaccg
     1741 cgtcggaatt gagcagcacc gcatcgtgcc ggcggttgcg cgcgagcgct cgattgaccg
     1801 agcccacaaa tcccagattg cgtgcgttgc gcagcagggt gatcgagccg ttgagctcca
     1861 attcatctag ggcggcggcg agctcggcgt ccggggtcgc gtcatccacc accacgacgc
     1921 gcgcatcgcc cgcggtggcg agcgcggcag tgatacacgc cagcgtttcg tcgcggccgc
     1981 ggtagacagg gatgatcaca tcgacgcggg ggcgatttag cccaaactgg gtgcggccgc
     2041 gggcggtgag cgcgggtttc caacgcaaca gacgcggtgc ggtgccaccc ggcagtcgca
     2101 gcgccggctc gagcagcaac ggcatgccgg gcatcgattg ccggtcacca ttgggaagca
     2161 gggccgagat gtgcaagcgg ttcccggaaa cgggtgtgcg tcgaaggtcg atgtgaaaat
     2221 gccagcgatg gttcgatcgc ggcttaagct cactgcgctt ctcaacacta tgaccgtgtt
     2281 cgtcctcgat tcgcaaggac acgggctcgc ccgccaacca gttgaggcgc acccagccgt
     2341 gcaccacgcg gccttggctt gaggtacggc cgtcaagacc gaaccgaggt gtgcttgagc
     2401 aaccgccgcc gatgagtgcc acgccgcggc tactcacctc gaggatggcg tgagcggcaa
     2461 ttgccggtag tttgaaatgg aagctaggat gaccgtattg cagcgaggtc ctgacgggtt
     2521 gcaggaatgc cgccccggtg tttaagcgga tctgcaaaat tttgaccggc tcggcgccga
     2581 cgagccggcc agtaaactcg agattcgctg cgcgaccgat ccacccggcc gcacgattgg
     2641 tcggatgttc tacgatctca cctgcgacgt gctcgagcag ccctccgggt acgatgcaaa
     2701 aatccgcaag cgccgactct agaatctgcc gcgcttcctc ggcgcggtca aggcacagca
     2761 gcgtgcggga cagcagcgca tgaacatcgg cgtccggggt ttttacgcgc tgcaatagcc
     2821 gcaacgctgc gtcgtgatca cggccctcga gcgacagaag cagcccatag atccagacca
     2881 gttccgcgct ttcgtccaac gatacgcgcc acgcacggtc aatgtggcga agcgcgtcag
     2941 ccacgcaatt cgcgcgcaga gccgcgaggc actgggtcag gtgtttttgc gtcgaccggc
     3001 tttgtcccgc gcggcaagtg ctctggattg ctctacaggc cgacgccgat accaagctcg
     3061 caacggaagg tcgttgcgga gcatcgccga gcgagacatc cgaaccgggt atcacagcca
     3121 aaccccaaga cctaccgtct cacgcccatt ggggaaatca gggcttgtgt ttcgtgatat
     3181 cggaagaatc gagtttggtg acaccttgaa gttcaatggt agtcttgccg ccatcgaggg
     3241 agatgtaggt attgccgcca ctgatgcgga tgtcgtgatg ttgctgcaga tacgacaacg
     3301 aatacccttc cagatacaac tgatcctgac cgccaatgaa attcgtgatg acgtgatgac
     3361 caccggcctc gctcgcgagg aactcaaata cattgtgatt ggcgccgccc gtcatggttt
     3421 ccatgcccga accaccgatg aaggtgtcgg gccctaagcc gcccttcatg atcgtggtgc
     3481 cggtgccgcc gataaattcg ttggagaaat tgccgccgac cagggtggcc ttcccatgca
     3541 gcgcatcctc gatctgctca ccgctcttgg tatcggtaaa cgccagggtg ccgccggata
     3601 tcgacgcact gccgaacacg ccgtgaacgg tggcgtggcc ggcctcgaag atcgtgtcgc
     3661 tgccgaatcc caaggtgatg gtatcgttgc cgctcagccc cgtttgatga acggtgcccg
     3721 aaccgagcaa tgtcagcccg tcttgaccgc tgcccaccgt gatggcgcct tggccgtcca
     3781 cggtaatcga atcgttgccg ttgcccgcgg tgacattgcc gccggaatat agggtaattt
     3841 tgtcgttacc gcttcccgcc ttaactgttg cccatccgcc gacgccgatg gtgtcgttgc
     3901 cgttgcccgc gctgacgttg ccgccggaat acagggtaat tttgtcgtta ccgcttcccg
     3961 ccttaactgt tccgaatccg ccgacgctga tggtgtcgtt gccgttgccc gccgtgacgt
     4021 tgccgccgga ataaatcgca atgctgtcgt tgccgttgcc cgcagtgact tttcctacgc
     4081 cgccgatggt gatcgtgtcg ttgccgttgc ctgcgatgac cgtgccattg ccgaatacga
     4141 cgacgttatg gtttccgcca cctacagtta tcgtcgttcc caacgcctcg ctcccttccg
     4201 cttacaacgc tgatctgcga ccgcagcggt tactcaaact cgaccgggtg atttgagaat
     4261 cgccgcgtcg tcaccgaatt gcatcccaca cgaattgatt taacaatatc tcgccacgta
     4321 tgccgcagat caattcgcat gcaatgttta acacgaaaac tgcgacaggg gtagcgagac
     4381 tgaacataaa aagtgtgaac cgatgcgctg cgatgacgtt cacgttgtgt ttcgtggtga
     4441 agcgtttttc cagcacattt gtgtaggaca tttgagcgcg atgaatcatt tgtgcgcatc
     4501 cagaattgag cacatgcccg caaatcgtga tcgtctccgc agcatcgaag cacttatgtc
     4561 gtcaaccgcc cgcattgccg cgaatctttg aacgccggca ttaattttca ctcgcggtag
     4621 gctagtctag gagatgtcgg agcgctatcg ttccgccgaa atgcatgcat aaactttagt
     4681 cggggtctcc gatcgaatgg ccatatgaca actctgtttc gagcgtatga gctgctggcg
     4741 agctcggagc tattcgatgc cgagtactac gtcagacaca atccggatat tgccgcgctc
     4801 aatgtcgatc cggtgctgca ttacctagag tcgggtgcgg ggagggttct gaatccttcc
     4861 gcccatttcg acagcgctta ttatttggaa cagtgtcttc gcaatggcga agtcacgagc
     4921 aatccgctgc tgcattacat caccgtgggg gcctcgcgcg gattgaagcc gctgcgtgac
     4981 gcgggcggcg ccgaaccgct tgcagcgcgg aatttgccgg gaggcttcgc aatccatgtg
     5041 gaccgtgcgg ccgtgattcg cggcgccgac ggatcgagca ggctcgccat cacgggttgg
     5101 gccgtcgcac aggccgcggt gtcgcgactc tctgcggctt gcaatgacgt gccgctcggc
     5161 gaggcgacgc tcgggatcgc gcgccccgat gtcgcgcagg cgtttccgga ccacgcgcat
     5221 gcggttaatg ccggttttag catcgtcgcg gattttacgg cgcctgaggg cagcgctcaa
     5281 ttgccgctca ccgtctcgct cttgaccgcg ggcggcgccg aacatcgaca ggttctgcag
     5341 atggagatcg attcgactcc gtcggctgaa gatgccgccg ccgtcgcgac gcgaaccgtg
     5401 cgggcgcgcg tcgagaagca tccgccaccg atgcaaatcg agatcgacga agcgatggtc
     5461 gatgatgccg ggattttgac ggtctccggt tgggccgtgt gcagcctcga tatcacttcg
     5521 gtcagcgtct gggtggacgg ggagatgatc ggaaccgcgg acttcgggcg tcggcgcgaa
     5581 gacgtggcgc gcgcccgcaa ggaatttccg cgggcattga attctgggtt tgcctataac
     5641 gcggacctca ccaggttcgg tgcgggcgag agaaccgtaa aagtcgaggc agttgcacaa
     5701 agcgggattc gccggcacgc cttggttcaa gtgctgattc cggagcccag cgtgccgctc
     5761 ggcgagccct tatttcattt tcactgcgat tcgatttcgc tctcgacggc gggcgccgtc
     5821 tatatcgaag ggtggctaat cggcgccacc accaccgaat ccctgacggt gatgttggac
     5881 gggatcgaca tcggcgctgc cagcatcggg cgcgagcgcc ccgatgtcgg tcaagcgttt
     5941 cccggtattt cctacgcacg caattcgggc tttacgtttc gccgcgcacc gggcagcgcc
     6001 gtggagccgg gcgaacatca catcgtgcta cacgtgcgcg atgcggcggg cgcggtgcgc
     6061 gaggttccgc tgccggtgtg ggcggcggag gcagcgccgg atggcgagaa gtcatcgccg
     6121 ctcgcggcgg gcgaggacgc cgatgcgatt gcgctgtcga tcgatgcacc ccgcatcagc
     6181 aatggccgcg ccgaaacccc gatcatcagc agcctgtcga tcaacggctg ggcgctggcc
     6241 ggtgcgggcg tcgccgaggt gagcattacg atcgacggcg cgccggtcgc caacgcgcag
     6301 catggcattc gccgcgacga tgtggcgagc gcctttcccg atcgtgtgga tgcgcagcaa
     6361 agcggatttg cgacgctcat acctcactgg ctgctggcca agggtgcgca ctcggtgtgt
     6421 atatcggtgc gggatcgggc ggatcatgtc gcgtcggtgc aatttggcat cgaagtcgaa
     6481 gaaattcccg aaggcgacgg accgtggtcc ctgcgccgca agatgagcca ggcggaaatc
     6541 gatctgcagc ttgcggtgct ctccggtctg gcctttcgcc cgtggttttc gattgtcatg
     6601 gcgtgcgacg gcgaggaggc gtcgctcgcc gcggtgcggc gcacgctgcg cagtctcgaa
     6661 gatcaagcct acggccattg gaacgtgtgg ttcttcgcgc gcggccagaa ggcgttgggc
     6721 aaacgctggc gcacggcgct gctcgccggc tttgaggccc tcgagggtcg gctcaagttc
     6781 gccgcgcctc gcgatgcgct caactttggg cgcgccgccg tgtgcccgcc cggaagttgg
     6841 gatggtgcgc tcgtcctgcc gcttttgagc ggcgatgaac tcggttgcga tgcgctgttg
     6901 gaattggcgc ttgccggcgg ccaaaatcgc ggttgcgatg tgttctacgg cgacgagcgc
     6961 tgccacagcg cggtcacgaa ttccgtgcag gcctacttta aacccggatg gtcgccggac
     7021 ctgttgctgt caacgaacta tatcgggcga ccgtggtgcg tgagcagcgc cgttctcgag
     7081 cgggccggat tgaacgtggg cgagtggctg cgacacggtg actacgattt cgtattgcgg
     7141 gcgaccgagg cggcggcggg cgtcggacac gtgccgaaag tgctctgtca gcgcggtccg
     7201 tgcgcggcgg agtccgaggc ccccgagcgc caggcgctcg ctcgggcgct cacgcggcgc
     7261 aagatttcag ggtcgatcaa gaaaggctgc cgccgcgggt tctatcgggt taaacccaat
     7321 aaggccaagt acggacgcgt ctcgatcatc attccgacgc gcgcatcgcg cggactcatc
     7381 aaggtgtgtc tcgagacgct caaggcgaag accgcctatc gcgactacga aatcatttgc
     7441 atcgaaaata ttccgcttga agacgcgcag tggaaggaat ggctgcgcgc gaacgcggac
     7501 aaagtgctga gcaccgacga acccttcaac tggtcgcgct acaacaaccg ctgcgcgcag
     7561 caggcgagcg gtgatttcct cttgtttctg aacgatgaca tcgaaatctt ggacaaggat
     7621 tggctcgaca cgctgctgca cgccgccgcg cggcccgagg tgggcatcgt cggaccgcag
     7681 cttctctatc cggacggcac ggtgcagcac gcgggcctgg ccttgaccaa cgtgatgggc
     7741 cgggcgcggc atatcttcag gcacggccgc gacaacgatc ccgggtattt cggcctggcg
     7801 ctcgcgcaac gcaatgtcat tgccgtgacc ggcgcctgca tgttgatgcg gcgcgagatc
     7861 ttcgatgcgc tgaacggttt cgatgaggcc tacagcgtca tcaacaacga tttggatttc
     7921 tgcctgcgcg cgtggcgacg cggcttgttg atcgtgtaca ccccgcacac gcggctcatc
     7981 catcacgaaa tggtgagccg tggcgcgctc ggcgatgcct tcgattcgac ggccttcatc
     8041 cgtgaatggc agggcgtgtt cgccctgggg gatccgtacc tgaaccgcaa tctgtcgcgg
     8101 gattcggatg atctggtgcc cgagcgtgaa ccggtgacgg ccatttgcac cggctacccg
     8161 ctgtttgccc gcagcgcaat tcgccgcatc ctggtgctaa agctcgatca catcggcgat
     8221 tgcattacct cgctgcccgc gatccgcaag ctcaagcggc atttccccaa ggcccgcatc
     8281 acggtgttga cggggacggc gaccaagctc gtttggtcga tggaaccggc cgtcgatcgc
     8341 gtactcgaat tcaatttttt ccacgcccga tcgcagttgg gcaaagtggg tgtttccgag
     8401 caggaactca acgacttggc cgagcaactc
//
LOCUS       DASWZT010000242         5382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_45432, whole genome shotgun sequence.
ACCESSION   DASWZT010000242 DASWZT010000000
VERSION     DASWZT010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5382
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45432"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1027
                     /locus_tag="VGE92_06680"
     CDS             137..1027
                     /locus_tag="VGE92_06680"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEY1313541.1"
                     /translation="MPPASQPWVSIRSGDLRAEIDPHGAQLSRLCDRDDRDLLWDGDP
                     AVWAGRAPLLFPIVGVLNGGRYRLGAQSYPLPRHGFARNRTFAVAASSISSATFRLSA
                     DAATLEVYPFRFELDAHFELQGATLSVQSTVRNLGDAALYASFGYHPALRWPLPFGEG
                     RASHFIQFANDEPAPARRIDADGLLTPARQPTPIVARRLALTDSLFQDDVLILDQVKS
                     RAVVYGAERGPRIRIDFPDAPYLGLWTKPGAAFICIEPWHGITDAEGFAGDFKEKAGV
                     FALPAGQSLSSTMALTLLQA"
     gene            complement(1053..2300)
                     /locus_tag="VGE92_06685"
     CDS             complement(1053..2300)
                     /locus_tag="VGE92_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY1313542.1"
                     /translation="MIFDEWIGQAWSLGASDLHVEAGTPLVARVRGELQTLGAVTQDT
                     LVQAAQDLLGGAGWRQFKERGSADMSIAIGGVRCRVNFYQTLRGVAVAVRLLAPSIND
                     LHGCNLHADFRKLIDATAGLIIISGPTGSGKSTTLAALIEEINATRARHIVTLESPLE
                     YIYSNRRSFIRQREIPTHSPSFEQGIIDALRENPDVLVVSEMRTPEVMRLTLNAAETG
                     HLVLATMHSATCAEALSRLCMSFPSDVQASVRAQLADCLVGLSCQRLDFLSAHRLRVP
                     RCEILLPSSGAKGTIRAGNFSQIANVLQSGGDEGMWTFDRYQRWMQQVTEWVRPVQTP
                     AQRGAADAHPPRAAAAARALRPSGEPATAAPLAARPAKAAPPRPSPGAPAQADDVFEV
                     PAEDMDLAQLAELAKKVVERKPS"
     gene            complement(2297..2821)
                     /locus_tag="VGE92_06690"
     CDS             complement(2297..2821)
                     /locus_tag="VGE92_06690"
                     /inference="COORDINATES: protein motif:HMM:NF021361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEY1313543.1"
                     /translation="MKISHWLLIVCLTASLACSATAFANDAPASDDSIREMLAITNAH
                     KLIDTIKLQINSLVAAAQQQAVQGKPLTPEKQAIIDRMQTRMQAALDEMLNWQTLEAM
                     YVRIYRASFTQDELDGMMAFYRTAAGQALINKMPLVTQNLMSEVQGQMKPMQEKIQQI
                     QREATQELKDLPAK"
     gene            complement(2848..3618)
                     /locus_tag="VGE92_06695"
     CDS             complement(2848..3618)
                     /locus_tag="VGE92_06695"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY1313544.1"
                     /translation="MAGTQSWFVWYELMTSDAAAAKVFYSGVTGWKTDDWPMPGMTYT
                     LLRVGERQVGGLMTMPSEACAAGMRPCWVGYIGVDDVDAAAAKAQRLGGKIIAAPSDI
                     PNVGRFAVVADPQGAAFNLFKPSQSGERAPVSSDIGQVGWHELHTREWPKAFEFYSEM
                     FGWLKGDAVDMGPMGSYQLFTCGGTPIGAMFNSPAAANARFWLFYVNVGDIDAAAARV
                     TGGGGKILQGPIQVPGGQWIIQAADPQGAGFALLGSRK"
     gene            complement(3694..3840)
                     /locus_tag="VGE92_06700"
     CDS             complement(3694..3840)
                     /locus_tag="VGE92_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313545.1"
                     /translation="MLILAAIARFTPAREPPANDLLAQSAIGHVGLTIIALVYVLVTL
                     WQRG"
     gene            4176..>5382
                     /gene="eno"
                     /locus_tag="VGE92_06705"
     CDS             4176..>5382
                     /gene="eno"
                     /locus_tag="VGE92_06705"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEY1313546.1"
                     /translation="MNAVQNSPIRLIRALEILDSRGVPTLRVAVHLENGLVGTASVPS
                     GASTGENEAVELRDADLGRYGGKGVRQAVANVNDVIAPRLTGCDPTQQAQLDRAMIDM
                     DATANKSKLGANAILGVSQAVARAAAMACGLPLYVYLGGAGTRRLPVPMMNVINGGKH
                     ADSSLDFQEFMIVPRGAPNFREALRYGAETFQALKSLLHERGHSTAVGDEGGFAPQLK
                     SNEEACELIVAAIERAHYRPGEDIAIALDPAASSFFENGKYRLTRSRQGDKSADEMTA
                     MYSSWVDRFPIISLEDGLAENDWQGFRRQTAVLGEHIQIVGDDLLVTNPIYIERAIAE
                     KSCNAALIKLNQIGTVTETIDAIHLCRKAGWGFVISHRSGETEDSFIADFAVAMGGGQ
                     IKTGSLCRSE"
BASE COUNT          960 a         1691 c         1678 g         1053 t
ORIGIN      
        1 tcgtcggctt gagcatcaag catgccgcag gcgacaccgc agagcaccag cagcaacgca
       61 agccttttca ttgaacttgt tccctgggag tgtctaccct attgctcccg gggccaggat
      121 atcgcagggt gtccatatgc cgccagcatc gcagccctgg gtttcgatcc gttccggcga
      181 tctgcgcgcc gagatcgacc cgcacggagc gcagctgtcg aggctttgcg accgcgacga
      241 tcgagacctt ttgtgggatg gcgatccggc cgtgtgggcc gggcgcgcac cgctgctttt
      301 tccgatcgtg ggcgtgttga acggcggccg ctatcgtctg ggtgcgcaga gctacccgct
      361 gccgcgacac ggattcgcgc gcaaccggac gtttgcagtc gccgcgagca gcatctcgag
      421 cgcgacattc aggttgagcg ccgacgccgc cactctcgag gtctatccat ttcgcttcga
      481 actcgatgcg catttcgagc ttcagggcgc aacgctctcg gtgcagagta ccgtccgcaa
      541 cctaggcgat gccgccttgt acgcaagctt cggttaccac ccggcgctgc gctggccgct
      601 gccgtttggc gaggggcgag catcgcattt cattcagttt gcaaatgacg agccggcccc
      661 ggccagacgc atcgacgccg acggtctgct gacgcccgcg cgtcagccga cgccgatcgt
      721 tgcgcggcgc ctcgcattga cggattcgct gtttcaagac gatgtgctca tcctcgacca
      781 agtcaagagt cgtgcggtcg tttatggcgc cgaacgcggt ccgcgaattc gaatcgactt
      841 tcccgatgcg ccgtatctcg ggctctggac caagcccggc gccgcgttca tctgcatcga
      901 gccgtggcac ggaattaccg acgccgaagg cttcgcgggc gacttcaagg aaaaggcggg
      961 cgtgtttgcg ctgccggccg gtcaatcgct gtcttcgaca atggcgttga ccttgctgca
     1021 agcgtgacca cgacgccctg actccgccgc cgctaggagg gcttgcgctc cacgactttc
     1081 ttggcaagtt cggcgagttg cgccaagtcc atgtcctccg cgggaacttc gaacacatcg
     1141 tcggcctgcg ccggcgcccc ggggctcggc cggggcggcg cagctttcgc aggccgcgcc
     1201 gcgagcggcg cagccgtcgc gggctcgccg cttggccgca gcgcgcgcgc cgccgctgcg
     1261 gcacgcggcg gatgcgcgtc cgcggccccg cgctgcgcgg gcgtttgcac cgggcgcacc
     1321 cactcggtga cctgctgcat ccaccgctgg tagcgatcga acgtccacat gccctcgtcg
     1381 ccgcccgact gcagcacatt ggcgatctgg ctgaagtttc ccgcgcgaat ggttcccttg
     1441 gcgccggaac tcggcagcaa tatttcgcag cgcgggaccc gcaagcgatg cgcgctcaag
     1501 aaatccagcc gctgacagct caatcccacc agacaatcgg cgagctgtgc gcgcacgctc
     1561 gcctggacat cggaaggaaa ggacatgcaa agccggctca acgcttcggc gcaggtcgcg
     1621 gagtgcatgg tggccagcac cagatggccc gtttccgcag cattcaaggt cagtctcatc
     1681 acttccggcg tgcgcatctc gctcaccacg agtacgtcgg gattctcacg cagcgcatcg
     1741 atgatcccct gctcgaagct cggtgaatgc gtcggaatct cccgttgccg gatgaacgag
     1801 cggcggttgc tgtagatgta ttcgagcgga ctttcgagcg tgacgatatg ccgggcacgc
     1861 gtcgcattga tttcctcgat gagcgcggcc aacgtcgtcg atttgccgga tcccgtcggc
     1921 cccgaaatga tgatcaaacc cgccgtcgcg tcgatgagct tgcgaaaatc cgcatgcaga
     1981 ttgcagccgt gcaagtcgtt gatggacggc gccagcagcc taacggccac ggccacgccg
     2041 cgcagcgttt gataaaaatt gacccggcag cgcacgccgc cgatggcgat cgacatgtcg
     2101 gcggagccgc gctccttgaa ctgtctccag ccggcgccgc cgagcaagtc ttgagccgcc
     2161 tgcaccagcg tgtcctgggt cacggcgccg agcgtctgca actcgccgcg cacgcgcgcg
     2221 accagcggag tgccggcttc cacgtgcagg tcgctcgcgc cgagcgacca cgcctgcccg
     2281 atccactcgt cgaagatcat tttgccggca gatccttgag ctcttgcgtc gcctcgcgct
     2341 gaatttgctg aattttctcc tgcatcggct tcatttgacc ctgcacttcg ctcatcagat
     2401 tctgcgtgac gagcggcatt ttattgatca gcgcctggcc cgcggcggtc cgataaaacg
     2461 ccatcattcc atcgagttca tcctgggtga aggaagcccg ataaattctc acgtacatgg
     2521 cttccagggt ttgccaattg agcatctcat cgagagcggc ctgcatccgg gtctgcatgc
     2581 ggtcgatgat ggcttgtttc tcgggagtca acggtttgcc ctgcacggcc tgctgctgtg
     2641 ccgccgccac cagcgaattg atctgcagct tgatggtgtc gatcaatttg tgagcgttcg
     2701 tgatcgcgag catttcgcgg atcgagtcat cgctcgccgg tgcatcgttg gcgaacgccg
     2761 tcgcgctgca cgcgaggctt gccgtcaggc agacgatgag cagccagtgt gaaatcttca
     2821 agagactctc ctcaggtttt cggccggcta ttttcgcgag ccgagcagtg cgaacccggc
     2881 gccctgtggg tcggcggctt ggatgatcca ctgaccgccc ggaacctgaa tcggcccctg
     2941 caggatcttg ccgccgccgc cggtgacccg cgccgcggcg gcatcgatgt cgccgacgtt
     3001 gacgtagaac agccagaatc gggcgttggc agcggctggg ctgttgaaca ttgcgccgat
     3061 cggcgtgccg ccgcaggtga acaattggta gctgcccatg ggtcccatgt cgaccgcgtc
     3121 tcctttgagc cagccgaaca tctcgctata gaactcgaac gccttgggcc actcgcgggt
     3181 atgcagttcg tgccagccga cctgtccgat gtcgctcgag accggcgcac gctcaccgct
     3241 ctggctcggt ttgaacaaat tgaaggccgc gccctgcggg tcggccacga cggcgaaacg
     3301 gccgacgttg gggatatcac tcggcgccgc gatgatcttg ccgccgagtc gctgcgcttt
     3361 ggcagccgcg gcgtcgacgt cgtcgacccc gatgtatccc acccaacaag gtcgcatgcc
     3421 ggcagcacag gcctcgctcg gcatcgtcat caagccgccg acttggcgct cgcccacgcg
     3481 cagcaacgtg taggtcatgc ccggcatcgg ccagtcatcg gttttccagc cggtcacgcc
     3541 gctgtagaac accttcgccg ccgcggcgtc gctggtcatc aactcgtacc aaacaaacca
     3601 actttgagtg ccggccatgc ccctctccta tgtgggttat ggcgcatagg ctagcagttc
     3661 gagggcatcg agcaaggttt cgaggggcgg cggctagcct cgttgccaga gcgtcacgag
     3721 gacgtaaacc aaggcgatga tggtgagccc cacgtgtccg atggccgatt gcgccaacaa
     3781 gtcgttggcg ggcggttcgc gggcgggtgt gaatcgggca atggcagcga ggattaacat
     3841 ggcgactata cgataaacgc gcccccggtt gaaagcgtga ttcgtctcac gactttttga
     3901 agccgggcgg tgcccggtgg cctgtgccgt tatgagtgat tatcaattcg catatttgac
     3961 caatggagcg tcacggaaga cgcgacccgg cggcgacgga ttcgcggcgc cagcggagca
     4021 gggcgcgagg cgcgacatga tgccggctca cgccgcgctc attgtctttc tgggtatttc
     4081 cactggaaat ggcgattgag cgatccctgg aaagtatcgg catggtggta attgctggcg
     4141 gccattttaa atcggatcaa tggtaggctg ccgagatgaa tgcagtccag aattctccca
     4201 tccgactgat tcgcgccctg gagattcttg attcgcgcgg ggtccctacc ttgcgtgtag
     4261 cagtacattt ggagaatggt cttgtcggca ctgcatcggt gccttcggga gcttccaccg
     4321 gcgagaacga ggcagtcgaa ctacgggacg cggatttggg ccgctatggt ggcaagggcg
     4381 tgcggcaggc ggtcgccaat gtaaacgatg tcatcgcgcc gcggctcacc gggtgcgacc
     4441 ccacgcaaca ggcgcaattg gatcgcgcaa tgatcgacat ggacgccacg gcaaacaagt
     4501 ccaagctcgg cgccaatgca atcctgggtg tatcgcaggc cgtcgcgcgt gctgcggcga
     4561 tggcctgcgg attgccactc tacgtctatc ttggcggcgc gggcacgcgg cgccttccag
     4621 tgcccatgat gaatgtgatc aacggtggca agcacgccga ttccagtctc gactttcagg
     4681 agttcatgat cgtgccgcga ggcgcgccca atttccggga ggcgttacgc tacggtgccg
     4741 agacttttca ggcgcttaag agtctgttgc acgagcgagg tcacagcacg gcggtcgggg
     4801 atgaaggcgg cttcgccccg cagcttaaaa gtaacgagga ggcgtgcgaa ttgatcgttg
     4861 ctgcgattga acgcgcgcat tatagacccg gcgaggacat tgcgatcgca cttgatccgg
     4921 ccgcttcttc gttcttcgag aacggcaagt atcgcctcac ccgcagccga cagggcgaca
     4981 agagcgcgga cgaaatgacc gccatgtatt ctagttgggt cgatcgcttt ccgatcatat
     5041 cgctcgagga tggtctcgcg gagaacgact ggcagggctt tcgccgccag acggccgttc
     5101 tgggcgagca cattcagatt gtcggcgacg acctgctggt gaccaatcca atctacatcg
     5161 agcgcgccat tgccgagaag agctgcaacg ccgccctgat caagctcaat caaataggca
     5221 ccgtgactga gaccatcgac gctattcact tatgccgcaa ggctggctgg ggctttgtca
     5281 tctcacatcg ctccggcgaa acggaagaca gcttcatcgc agattttgcg gtagccatgg
     5341 gcgggggcca gatcaagacc ggttcgctgt gccgctccga ac
//
LOCUS       DASWZT010000243         5225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_45443, whole genome shotgun sequence.
ACCESSION   DASWZT010000243 DASWZT010000000
VERSION     DASWZT010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45443"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VGE92_06710"
     CDS             complement(<1..558)
                     /locus_tag="VGE92_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313547.1"
                     /translation="MKLLNCAALAAVIMLSTAAAGAAPAPGASWAEGVNYFTVVPQHI
                     PSLTAGKVEVTEVFSYACPACNLFVPTIHKLKASLPPNAVLDYLPAAFNPSEDWPVFQ
                     LGYLTAVTLGVADQTHDAMFNAVWQGGDLAVIDPATSGLKRHMPTIEDIAKFYKARAG
                     VPEEKFIETSKSFSVDVKVRATEALI"
     gene            complement(599..1900)
                     /locus_tag="VGE92_06715"
     CDS             complement(599..1900)
                     /locus_tag="VGE92_06715"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY1313548.1"
                     /translation="MRHKIVLATMGSLGDLHPFIALGCALRDRGVNVVLACATEYGSK
                     ANAAGLQFHALRPSFADVERDLGMDRAQLTQRAVAHSAFLFRQLVLPYVRMAYDDMLE
                     ATAGADLVLPSSLAFGARLAAETRAIPWIAVVLQPMMFLSAYDPPLIPQAQSLSALLR
                     RLGPAPTRAALWVLKKAVAAVFRPLESLRADLGLPPGGKDPLFDGQFSADGAIGLYSK
                     LLGEAQPDYPRPSSIAGFATFDSETGLTQGIDPELDAFLNAGAAPLVFTLGSLIINSP
                     GNFYRESLGVARALQRRAVLLVGDAARGDLARLQSAEVFICSYAPHSLLMPRAAAVIH
                     HGGIGTLAQALHSGRPQLIVPFFADQPDNAARAARLGVARIVPARRYSARRASREIAC
                     LLGGDRYLVRARAVGDALAAEDGAAEGARIVLDRLERLRGK"
     gene            2026..3351
                     /locus_tag="VGE92_06720"
     CDS             2026..3351
                     /locus_tag="VGE92_06720"
                     /inference="COORDINATES: protein motif:HMM:NF008589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HEY1313549.1"
                     /translation="MLNDPIDADPSRPGRGPAAFIRRHWVALLIVLVVLLLLVMLMRY
                     IQHKQPVAQGGPGAGPGGGRGGQNGPVAVTVATATVGDIQLRVPALGTVTPLANVTVR
                     TQISGVMQKILFTEGQLVHQGDSLAQIDPRPYEAALQQMQGNLKRDQALLADAKLDLK
                     RYEGLIKEDSIAQQQLDTQKALVDQYIGTIESDEGQVKTAAVNLLYTRILAPASGRVG
                     LRQVDPGNYVTPGDTNGIVVINQLQPITVIFPIPEDDVPALMQRVHSGPALQVEAYDK
                     PNTTKLAEGRLLTVDNQIDVTTGTVKLRAQFDNTDDQLFPNQFVNVQLLQELVRNQII
                     IPNSAVRRGAPNGTVSTFVYVVNSDRSVQVRPVTLGVVDGERVAVTKGLAAGELVVTE
                     GADRLREGAKVQLPDATPPPAAARPANGAKSGTPNAPGSHKHTRPKPSQ"
     gene            3374..>5225
                     /locus_tag="VGE92_06725"
     CDS             3374..>5225
                     /locus_tag="VGE92_06725"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1313550.1"
                     /translation="MNPSRIFILRPVATTLLMVAILIVGAIAYLQLPLSALPEVAYPT
                     IQVQTFYPGASPEVITSAITAPLEKQFGQMPGLSQMSSTSSAGASVITLQFSLALAID
                     VAEQQVQAAISAAQNLLPQDLPAPPVYAKVNPADAPVLILGLTSKVMPLTEVEDLADT
                     RVAQKISQIKGVGVVSISGGQRPAVRVVVNPRAMAAYGLNLDDLRTTISNANQNGPKG
                     TLDGPSTAYTVNTNDQIRNADDYGAIVIAYKNGSAVRLRDVADLKSGAENAKLGGWMN
                     SVPALILNVQRQPGANVVDVVDRIHALLPSLQAALPSGVDLAILTDRTTTIRASVADV
                     EFELVLAVILVVLVIYVFLRNVPATIIPSLSVPLSLIGTFAVMYLANFSLNNLSLMAL
                     TIATGFVVDDAIVMIENISRYIEQGEAPLEAALKGAGQIGFTIISLTVSLIAVLIPLL
                     FMQDVVGRLFREFAVTLAVTILISALVSLTLVPMMCAKLLKARPAGRHSDLEAGRWFS
                     AVVEWYGRRLTWVLDHQRLTLLVATGTVVLTAILYILIPKGFFPEQDTGLIQGVSEAT
                     ESISYAAMADRQQALAAAILKDPDVLSLSSYIGIDGNNVTLNSGRFLINLK"
BASE COUNT          993 a         1685 c         1606 g          941 t
ORIGIN      
        1 gatcaacgcc tcggtggcgc gcactttgac atccacgctg aagctcttcg aggtctcgat
       61 gaacttctcc tccgggacgc cggccctggc cttgtagaac ttggcgatgt cctcgatggt
      121 cggcatgtgg cgcttgagtc cggaagtcgc aggatcgatg accgccaagt cacccccctg
      181 ccagacggcg ttgaacatgg catcgtgcgt ctgatcggca actccgagag tcacagcggt
      241 caaataccct agctgaaaca ccggccagtc ctcgctcgga ttgaaagccg cgggcaagta
      301 atcgagtacc gcattcggcg gcaacgatgc tttcagcttg tgaatcgtcg gtacgaacaa
      361 attacatgcc ggacaggcat aggaaaacac ttcggtgacc tccaccttgc cggccgtcaa
      421 cgacgggata tgctgcggca cgaccgtgaa ataattcact ccctccgccc atgaggcgcc
      481 aggcgccggc gccgcgccgg ccgcggctgt gctcaacata atcactgccg ccaacgcggc
      541 gcaattcaac agtttcatcg acgctcctta ccagtcgtgg aattccgcat ccgaaggctc
      601 attttcccct gagtcgttcc agcctgtcga gaacaatacg cgcgccctcg gcagcaccgt
      661 cttcggcggc gagcgcatcg cccaccgctc gtgcccgtac gaggtatcga tcgccgccga
      721 gcaagcaggc gatttcgcgg cttgcacgcc tcgcggagta gcggcgcgcc gggactatgc
      781 gcgccacgcc gagccgcgct gcgcgcgccg cattatcggg ctgatccgcg aaaaacggca
      841 cgatcaactg cggccggccg gagtgcagcg cctgcgccaa cgtgccgatg ccgccgtgat
      901 ggatcaccgc ggcggcgcgc ggcatcagca acgagtgcgg cgcgtaactg cagatgaaga
      961 cttccgcgga ctgcaaccgc gcaaggtcgc cgcgcgctgc atcgcccacc agcagcacgg
     1021 cgcgccgctg caaggctcgc gcaacaccga gactttctcg ataaaaattc ccgggactgt
     1081 tgatgatgag actgccgagc gtgaacacga gcggtgccgc gcccgcattc aggaacgcat
     1141 ccaattcggg atcgataccc tgggtgagac ccgtttcgct gtcgaaagtt gcgaagccag
     1201 cgatggatga gggtcgcgga tagtccggct gcgcctcacc cagcaacttg gaatacaggc
     1261 cgatcgcccc gtccgcggag aattgcccgt cgaacagggg atctttcccg cccggcggca
     1321 gcccgaggtc cgcgcgcagg ctctcgagcg gccgaaacac ggcggcaacg gccttcttga
     1381 gcacccacag cgcggctcga gtcggcgccg ggccgagtcg ccgcaacagc gcgctcaagc
     1441 tttgcgcttg cggaatcagc ggcggatcgt acgcggacag aaacatcatg ggctgcagca
     1501 ccaccgcgat ccatgggatg gcgcgcgttt cggcggccag tcgggcgccg aacgccagac
     1561 tgctcggcag cacgagatcg gcgccggccg tcgcctccag catgtcgtcg tacgccattc
     1621 gcacatacgg cagcaccagc tgccgaaaca ggaacgcgct gtgcgcgact gcgcgctgcg
     1681 tgagctgcgc gcgatccatt ccaaggtccc gctccacgtc cgcgaagctc ggccgcagcg
     1741 cgtggaattg caggcccgcg gcgttcgctt tgctgccgta ctcggtggcg caggcgagca
     1801 ccacgttcac cccgcgatcg cgcaaagcgc aacccagcgc gataaacgga tgcagatcgc
     1861 cgagtgaacc cattgtcgcc agtacgatct tatgccgcat gcgctaacta cttcctgcag
     1921 agagtggttg ttcgccggca aatctggtta tagtctcgaa tcgcccgaac gtccctatcg
     1981 ccagtgtatg ccggacgtgg gcctgatgtg acgaagtgtg aagttgtgtt aaacgatccg
     2041 atagacgcag atccttcccg gcccgggcgt ggccccgcgg cattcatccg tcgccactgg
     2101 gttgcgctgc tcattgttct cgtcgtgctg ctgttgctgg tgatgctgat gcgttatatc
     2161 cagcacaagc aacccgtggc gcagggagga ccgggcgcgg gccccggcgg cggacgcggc
     2221 ggacagaatg ggccggtggc cgtgaccgtc gcgactgcca cggtcggcga cattcaattg
     2281 cgcgttcccg ctctcggtac ggtcacgccg ctcgccaacg tcacggtgcg cacgcagatc
     2341 tccggagtca tgcaaaagat cctgttcacg gagggtcagc tcgtgcacca aggcgactcc
     2401 ttggcgcaga tcgatccgcg tccctacgaa gccgccttgc aacagatgca aggcaatttg
     2461 aagcgcgacc aggcattgct cgcggacgcc aagttggacc tgaagcgcta cgagggactg
     2521 atcaaagaag attcgatcgc gcaacagcaa ctcgacacgc aaaaggcgct ggtcgatcaa
     2581 tacatcggta cgatcgaatc ggacgaggga caggtcaaga ccgccgcggt caatctgctg
     2641 tacacgcgca tcctggcgcc ggcgagcggc cgggtcggat tgcgccaagt cgatccgggc
     2701 aactatgtca cgccgggcga caccaacggc atcgtcgtca tcaaccagct gcagccgatc
     2761 accgtgattt tcccgatacc cgaggacgac gtgccggcgc tgatgcagcg cgtgcacagc
     2821 ggccccgcgc tgcaggtgga agcgtacgac aagcccaaca ccaccaagct cgccgagggc
     2881 aggctgctca ccgtggataa tcaaatcgat gtgacgacgg gtactgtgaa gctgcgtgcg
     2941 caattcgaca ataccgatga tcagctgttc cccaatcagt tcgtcaacgt ccagttgctg
     3001 caggagctgg tacggaatca aatcatcatc ccgaactccg cggtgcgccg cggcgcgccc
     3061 aacggcacgg tctcaacgtt cgtctacgtc gtgaattcgg accgcagcgt acaggtgcgg
     3121 ccggtgacac tcggtgtcgt cgacggcgag cgtgtcgccg tgaccaaagg gctcgctgcc
     3181 ggcgaactcg tggtcacgga aggcgccgac cgtttgcgcg aaggcgccaa ggtgcaactg
     3241 ccggatgcga cgccgccgcc tgcggccgcg cgtcccgcca acggcgcgaa gtcgggaacg
     3301 ccgaatgccc cggggtcgca caagcacacg cgccccaaac cgtcgcaatg aggccggacg
     3361 cggtcgcgcg cgcatgaacc cctcacgtat ttttatcctg cgcccggtcg cgaccacctt
     3421 gttgatggtg gcgatcctga tcgtgggcgc gatcgcctat ttgcagttac ctctctcggc
     3481 gttgccggaa gtcgcgtatc cgaccatcca agtacagacc ttctaccccg gcgcgagtcc
     3541 cgaggtcatc acctcggcga tcacggcgcc actcgagaaa caattcggcc aaatgccggg
     3601 cttgagccag atgtcctcga cgagctcggc cggcgcctcg gtgatcacgc tgcagttctc
     3661 cttggctctc gccatcgatg tggccgaaca gcaggtgcaa gcggcgatca gcgccgcgca
     3721 aaatctgctg ccgcaagatc tgccggcgcc tccggtttac gccaaggtca atcccgccga
     3781 tgcgccggtg ctgatcttgg ggctgacctc caaggtgatg ccgctcaccg aggtcgagga
     3841 tctggccgac acgcgcgtcg cgcagaagat ctcgcagatc aaaggggtcg gcgtcgtcag
     3901 catcagcggc ggtcagcgcc cggcggtgcg cgtggtggtg aacccgcgag ccatggcagc
     3961 ctacgggctc aatctcgacg acctgcgcac caccatcagc aacgccaatc agaacgggcc
     4021 gaagggcacg ctcgatgggc cgagtaccgc ctacacggtc aacaccaacg atcaaatcag
     4081 gaacgcggac gactacggtg caatcgtgat cgcgtacaag aatggttcag cggtcaggct
     4141 tcgcgatgtg gccgatctta agtccggcgc ggagaacgcc aaacttggcg gttggatgaa
     4201 ctcggtgccg gcgctgatct tgaacgtgca gcgtcagccg ggcgcgaacg tggtcgacgt
     4261 cgtcgatcga atccacgcgc ttctgccctc gttgcaagcc gcattgccct cgggagtcga
     4321 ccttgccatc ctcaccgacc ggaccacgac catccgcgcc tcggtcgccg acgtggaatt
     4381 tgaactggtg ctggcggtga tcctggtcgt gctggtgatc tacgttttcc tgcgcaatgt
     4441 tcccgcgacc atcatcccca gcctgtcggt gccgctgtcg ctcatcggca ccttcgccgt
     4501 gatgtatttg gcgaatttca gtctcaataa cctgtcgctg atggcgttga cgatcgccac
     4561 gggattcgtg gtcgacgatg cgatcgtgat gatcgagaac atttcgcgct acatcgagca
     4621 gggcgaagcg ccgctcgagg cggcgttgaa aggcgccggg caaatcggct tcaccatcat
     4681 ttcgcttacc gtgtcgctga tcgcggtgct gataccgctg ctcttcatgc aggatgtggt
     4741 cggccggctg ttccgggaat ttgccgtcac cttggcggtc acgattctca tatcggccct
     4801 ggtgtcgctc accttggtgc cgatgatgtg cgccaagctg ctcaaggcgc ggcccgcggg
     4861 ccggcattcc gacctcgagg cgggtcgttg gttcagtgcc gtggtcgaat ggtatggccg
     4921 cagactcacc tgggtactcg atcaccagcg attgaccctg ctggtggcga ccgggacggt
     4981 ggtgctgacg gcgattcttt atattctcat tccgaagggt tttttcccgg agcaggatac
     5041 gggattgatt caaggcgtct cggaagccac cgaatccatc tcctacgcgg ccatggcgga
     5101 tcgtcagcag gcattggcgg ccgccattct caaggatccg gacgtcttga gcctatcttc
     5161 gtacatcggc atcgacggca ataacgtcac gttgaatagc ggtcgcttct tgatcaacct
     5221 gaagc
//
LOCUS       DASWZT010000244         2236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_88869, whole genome shotgun sequence.
ACCESSION   DASWZT010000244 DASWZT010000000
VERSION     DASWZT010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88869"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..1036
                     /gene="lipA"
                     /locus_tag="VGE92_06730"
     CDS             89..1036
                     /gene="lipA"
                     /locus_tag="VGE92_06730"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEY1313551.1"
                     /translation="MAVVYDINSPRPRHPEKANRPDSAVQKKPDWIRVKAPVSPGYFA
                     TQKIMRDNRLVTVCEEAGCPNIGECWEKKNATFMIMGDTCTRACAFCNVKTGLPGALD
                     PNEPAHVGEATAKLGLAHIVVTSVDRDDLADGGAQHFADTIAAIRARCRHTTIEVLTP
                     DFLRKDGALELVVAARPDVYNHNLETVPSLYLSVRPGARYFHSLRLLQRVKEIDTAMF
                     TKSGIMVGLGEERNEVLQVMDDLRSADVDFLTIGQYLQPTRKHHEVMRFVPPDEFKAL
                     ETIAYTKGFLMVSASPLTRSSHHAGEDFARLKAARAAQV"
     gene            1044..1505
                     /locus_tag="VGE92_06735"
     CDS             1044..1505
                     /locus_tag="VGE92_06735"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY1313552.1"
                     /translation="MPQFSTKRRVRHAAANMFDLVIDAEKYPQFLPMCEALKINSRTD
                     KGNGVTVMVAEMTVAYKLIRQTFATRATADRPNLKVLVEYLDGPFSRMQNRWAFHPAG
                     ENACDVEFFIEYEFKSRAFAMLAGALFDTVFRKYAAAFEKRADAIYGRRSA"
     gene            1684..>2236
                     /locus_tag="VGE92_06740"
     CDS             1684..>2236
                     /locus_tag="VGE92_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313553.1"
                     /translation="MPTKLTAAKPEIQQRNPLREWLFRIGSYGGMLFGFSAVLLIWIG
                     AAYFTHNERTQTEQGALQNAGNLSRAFEEQIIRSIRSVDQTLLYVRDSYARDPQHFNM
                     SLWQRNNEFLTGITFQLAIIDESGLMVASNIPGSIPGVYLGDREHFTVHAQRNTDELF
                     ISKPVLGRVSKKWSIQMTRRVTMP"
BASE COUNT          451 a          710 c          664 g          411 t
ORIGIN      
        1 atcggcgcca tcgtgttcct gggcatctgg cgggcgatta gaaacgcctg accttgcggc
       61 gcggggcggc cagccgtaag taaagcccat ggcggtcgtc tacgacatca acagcccgcg
      121 tccgcgccat ccggaaaagg cgaaccggcc ggattcagcc gtgcaaaaaa agccggactg
      181 gatccgcgtc aaggcgccgg tgtcgccggg ctatttcgcc actcagaaga tcatgcgcga
      241 caaccgcctc gtcaccgtgt gcgaggaagc cggctgcccg aatatcggcg agtgctggga
      301 gaaaaagaac gccaccttca tgatcatggg cgatacctgc acgcgcgcct gcgccttctg
      361 caacgtcaag accggcttgc cgggcgcgct cgatccgaat gagccggcgc atgtcggcga
      421 agcgaccgcc aagttggggc tggcgcacat cgtcgtcacg tcggtcgacc gcgacgatct
      481 cgccgacggc ggcgcgcaac atttcgccga caccattgcc gcaatccgcg cgcgctgccg
      541 gcacaccacc atcgaagttt tgacgcccga ttttctgcgc aaggatggcg cgctcgagtt
      601 ggtagtcgcg gccaggcccg acgtttacaa ccacaatctg gaaaccgtgc cgtcgcttta
      661 tctcagcgtc cgcccgggcg cgcgctattt tcattcgttg cgcctgttgc agcgcgtcaa
      721 ggagatcgac accgcgatgt tcaccaagtc gggcattatg gtcgggctgg gcgaggagcg
      781 gaacgaagtg ctgcaggtga tggacgacct gcgctcggcc gatgtcgatt ttctgaccat
      841 cgggcaatat ctgcagccga cccgcaagca ccacgaggtg atgcgcttcg tgccgccgga
      901 tgaattcaag gcgctggaga cgattgccta caccaaaggc ttcctgatgg tgtcggcctc
      961 gccgttgacg cgctcttcgc atcacgccgg cgaggatttc gcgcgcctca aggccgcgcg
     1021 cgccgcgcaa gtttgaaaaa ttaatgccgc aattctccac gaagcgccgc gtccgccacg
     1081 ccgcagcgaa catgttcgat ctggtcatcg acgcggagaa atatccgcaa ttcctgccga
     1141 tgtgcgaggc gctgaaaatc aacagccgca ccgacaaggg caacggcgtc accgtcatgg
     1201 tggcggagat gacggtcgcc tacaaattga tccgccagac ctttgccacc cgcgccacgg
     1261 ccgaccggcc gaacctcaag gtgctggtgg aatatctcga cggtccgttc agccgcatgc
     1321 agaaccgctg ggcgttccat ccagccgggg agaacgcctg cgacgtcgaa ttcttcatcg
     1381 aatacgaatt caagagccgc gctttcgcga tgctggcggg cgctttgttc gacaccgtgt
     1441 tccgcaaata cgccgccgcg ttcgagaagc gggctgacgc catttacggc cgacgttccg
     1501 catagccgga cttccaatgc gccgcccgta ctgcactacc gttggcgcac gcgcgatcct
     1561 taacgcttgc ttgcaaacaa ggccgcgctt tgcgcgtttg agttcagtca aagccgtaca
     1621 aatcacttta tgctagacgg cgagcgagcg tcgcaacacg cgctcggccg gataacgcaa
     1681 tcgatgccga caaaacttac cgccgccaaa ccggagatcc agcagcgcaa cccgttgcgc
     1741 gaatggctgt ttcgcatcgg ttcctacggc ggcatgttgt ttgggttctc cgcggtgctg
     1801 ctgatatgga tcggcgcggc ctatttcacg cataatgagc gaacgcagac cgagcagggc
     1861 gcgttgcaga atgccggcaa tctttcgcgc gcgttcgaag agcagatcat ccgctcgatc
     1921 cgcagcgtcg accagacgct gctatacgtg cgcgattcgt acgcacgcga tccgcagcat
     1981 ttcaacatgt cgctgtggca gcgcaacaac gagtttttga ccggcattac cttccaactt
     2041 gccatcatcg atgagagcgg tctgatggtc gcgagcaata tccccggctc aatcccgggg
     2101 gtctatctgg gcgaccgcga gcacttcacc gtgcacgccc agcgcaatac cgacgaactg
     2161 tttatcagca agccggtgct cgggcgggtc agcaagaaat ggtcaatcca gatgacgcgc
     2221 cgggtcacca tgccgg
//
LOCUS       DASWZT010000245         2049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_21208, whole genome shotgun sequence.
ACCESSION   DASWZT010000245 DASWZT010000000
VERSION     DASWZT010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21208"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1280)
                     /locus_tag="VGE92_06745"
     CDS             complement(111..1280)
                     /locus_tag="VGE92_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005262059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEY1313554.1"
                     /translation="MANSAYREFNFGLGEVIDTVRDQVQRFAAERIAPRANEIDRSNL
                     FPRDLWRELGDLGLLGMTVSPDYGGAGLGYLAHLVAMEEISRSSAAVGLSYGAHSNLC
                     VNQLALNASEAQKRKYLPKLLSGEHVGALAMSEPGAGSDVVSMQLKAALHGDRYVLDG
                     RKMWITNGPEADVLVVYAKTAPQAGARGISAFIIEKDFKGFAAAQKLDKLGMRGSSTS
                     ELVFDGCEVPAENLVGQENEGVRVLMRGLDYERVVLSGGPLGIMAAALDVAVPYVRER
                     RQFGQAIGTFELVQGKLADMYASLNACRSYAYMVAGACDRADATRKDAAACILFCAEA
                     ATQAALDAIQMLGGNGYINDYPTGRLLRDAKLYEIGAGTSEIRRMLIGRELFGEG"
     gene            1371..>2049
                     /locus_tag="VGE92_06750"
     CDS             1371..>2049
                     /locus_tag="VGE92_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY1313555.1"
                     /translation="MAQIVSKIDVRSQEFAANRAAMQPLVEDLRATLARNAAGGSAAA
                     RHKHTESGKLLARERIAVLLDPGSPFLELSALAAQGVYGEALPGAGLVSGIGRVAGRE
                     CMVVANDPTVKGGTYYPLTVKKHLRAQEIAGDNALPCIYLVESGGAFLPKQDEVFPDR
                     EHFGRIFFNQARLSAQGIAQIAVVHGSCTAGGAYVPAMSDHAVIVRNQGRVFLGGPPL
                     VRAATGEE"
BASE COUNT          359 a          697 c          646 g          347 t
ORIGIN      
        1 gggcagcgca tcgccggtgc gcagcgagcg gccgacgatg cgctcttcga tgagattggc
       61 gatctggcgg tacgccgggc gatcgtcgag cggcacgaac agatccgcca tcagccctct
      121 ccgaacagct cgcggccgat cagcatgcgg cggatctccg aggtgccggc gccgatttca
      181 tacaacttgg cgtcgcgcag caggcgcccg gtcggatagt cgttgatgta accgttgccg
      241 cccaacattt gaatggcatc gagcgccgcc tgagtcgccg cctcggcgca aaacaatatg
      301 caggccgccg cgtccttgcg ggtcgcgtcg gcgcgatcgc acgctccggc caccatgtag
      361 gcatagctgc ggcaggcatt gagcgaggcg tacatgtcgg cgagcttgcc ctgcaccaac
      421 tcgaacgtgc cgatggcttg gccgaactgg cgccgctcgc gcacatacgg cacggccaca
      481 tcgagcgccg cggccatgat gccgagcgga cctcccgaca gcaccacgcg ctcgtaatcc
      541 aagccgcgca tcagcacccg cacaccctca ttctcctggc ccacgaggtt ctcggccgga
      601 acctcgcagc catcgaacac caactcgctg gtgctcgagc cgcgcatgcc gagcttgtcg
      661 agcttttgcg ccgccgcgaa tcctttgaag tccttctcga tgatgaaggc gctgatgccg
      721 cgcgcgcccg cctgcggcgc ggtcttcgca tacaccacca gcacatcggc ctccgggccg
      781 ttggtgatcc acatcttgcg gccgtcgagc acgtagcgat cgccgtgcag cgccgccttg
      841 agctgcatgg agacgacgtc ggatccggcg ccgggctcgg acatggcaag tgctccgacg
      901 tgctcgccgc tcaagagctt cggcaggtac ttgcgcttct gcgcttcgct cgcgttcagc
      961 gcgagctgat tcacgcacaa attcgagtgt gcgccgtaac tcaatccaac cgcggccgag
     1021 gagcgcgaaa tctcctccat cgccaccaga tgcgcgagat aacccaagcc ggcgccgccg
     1081 tagtcgggac tcacggtcat gccgagcagg ccgaggtcgc ccaattcgcg ccacaagtcg
     1141 cgtgggaaca gattgctgcg atcgatctcg ttggcgcgcg gcgcgatgcg ctccgcggcg
     1201 aagcgctgca cctgatcgcg caccgtatct atgacttcgc caagcccgaa gttgaactca
     1261 cggtatgcgg aattcgccat aactcaccgg acccccattg tcagcggacc ccaaatcgtt
     1321 ggataatcgg actgtcggac aattttgcca ctctcgaagc cacaaagcca atggcccaaa
     1381 tcgtctcgaa aatcgacgtc cgctcgcaag aattcgccgc caaccgggcc gccatgcagc
     1441 cgctggtcga ggacttgcgc gccacgctcg cgcgcaatgc cgctggcggc agcgccgcgg
     1501 cgcgccacaa gcacaccgag tcgggcaagc tactcgcgcg cgagcgcatt gccgtactgc
     1561 tcgatcccgg ttcgccgttc ctggagctgt cggcgttggc ggcgcaaggg gtgtatggcg
     1621 aggcattgcc cggcgccggc ctggtgagcg gcattggccg cgtcgccggc cgcgaatgca
     1681 tggtggtggc gaacgacccg accgtgaagg gcggcaccta ctatccgctc acggtcaaga
     1741 agcacctgcg cgcccaggag attgccggcg acaacgcgtt gccgtgtatc tatctcgtgg
     1801 agagcggcgg ggcattcttg ccgaagcagg atgaggtatt tccggaccgc gaacatttcg
     1861 gccgcatatt tttcaatcag gcgcggctct ccgcgcaggg catcgcgcaa attgccgtgg
     1921 tgcacggctc ctgtaccgcc ggtggcgcgt atgtgccggc gatgtcggac cacgccgtca
     1981 tcgtgcgcaa ccaagggcgg gtgtttctcg gcggcccgcc gctggtgcgt gctgccaccg
     2041 gcgaagagg
//
LOCUS       DASWZT010000246         2701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_40684, whole genome shotgun sequence.
ACCESSION   DASWZT010000246 DASWZT010000000
VERSION     DASWZT010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40684"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="VGE92_06755"
     CDS             <1..1045
                     /locus_tag="VGE92_06755"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY1313556.1"
                     /translation="STPIGQTSLGNYIFAGLQTQNWVFVLFGCVAAAGLALIVDQLLA
                     LMERGAGSHRRSMVAAGGIGLALIVLAALLPGFNRAQGSYLIGAKPFTEQYVLAALIE
                     QRLQARGLSAQGRNGLGSGVIFNALAANDIDAYVDYSGTLWANQLRRSDIKPRADVLT
                     EVGRWLETTHGIRMLGSLGFENAYALAMPRERAQKLGIRSIADLSAHAGELSIAGDYE
                     FFERPEWQAIHDAYGLGFRAQRTMQSDFMYQAVKADDVDVVAAYTSDGRIAQFDLAVL
                     DDPKHAIPPYDAILLLAPKRAHDEALIAALQPLIGAVPVALMREANLRASNGATSAGE
                     VARWLDDEIAKTR"
     gene            complement(1049..1423)
                     /locus_tag="VGE92_06760"
     CDS             complement(1049..1423)
                     /locus_tag="VGE92_06760"
                     /inference="COORDINATES: protein motif:HMM:NF018684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HEY1313557.1"
                     /translation="MLRITVIAAAALIGAAAFAFAGDQGEPDKGCDGGTYEMVDCLNA
                     KAAQWDKRMAIAYQRGLHAGEPKQREQLRVAQRLWIQYRDANCLYYGLGEGTIARIDA
                     SECMRSMTEARAEELEGKEDRN"
     gene            complement(1429..2448)
                     /gene="ilvC"
                     /locus_tag="VGE92_06765"
     CDS             complement(1429..2448)
                     /gene="ilvC"
                     /locus_tag="VGE92_06765"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004672686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEY1313558.1"
                     /translation="MRVYYDRDADLNLIKGKKVAIIGYGSQGHAHALNLRDSGVKEVA
                     VALRKTSAGVKKAEAEKFKVMEVAEAAKWADVMMMLTPDELQGDIYRESLAKNMKDGA
                     ALMFAHGLNVHFNLIEPRADLDVLMIAPKGPGHTVRSEYQRGAGVPCLVAIHKDASGN
                     AHDLGLSYASAIGGGRAGIIETTFKEECETDLFGEQTVLCGGLVELMKAGYETLVEAG
                     YAPEMAYFECVHEVKLIVDLIYEGGIANMNYSVSNTAEYGEYVSGPRVVNAETKAEMK
                     RILGDIQSGRFTRDWMLENKVNQTSFKATRAKLAEHPIEQVGAKLREMMPWIKQRALV
                     DKSKN"
BASE COUNT          447 a          862 c          841 g          551 t
ORIGIN      
        1 gtcaacgcca atcggacaga ccagcctggg caattacatc ttcgcaggct tgcagactca
       61 gaattgggtg ttcgtgctgt tcggctgcgt ggcggcggcg gggctggcgc tcatcgtcga
      121 tcaactgctg gcgttgatgg agcgcggcgc cgggtcgcat cgccgaagca tggtggccgc
      181 cggcgggatc ggcctggcgc tgatcgtgct ggccgcgttg ctgccaggat ttaaccgcgc
      241 gcaaggcagc tacctgatcg gcgccaagcc gttcaccgag caatacgtgc tggccgcgtt
      301 gatcgagcag cgtcttcaag cgcgcggctt gtccgcgcaa gggcgcaacg ggctcggctc
      361 gggcgtcata ttcaacgcgc tcgccgccaa tgacatcgat gcctatgtgg attattccgg
      421 cacgctttgg gccaaccagc tgcgccgaag cgatatcaag ccgcgcgccg acgtgctgac
      481 cgaggttggc cgctggctcg aaaccacgca cggcatccgc atgctcggct cgctcggttt
      541 cgagaatgcc tatgcgctgg caatgccgcg cgagcgcgcg caaaaactcg gcattcgctc
      601 gatcgccgat ttgtcggcgc atgctggcga attgtcgatt gccggcgatt acgagttctt
      661 cgagcgcccg gaatggcagg cgatccacga cgcctatggt ctgggtttcc gcgcccagcg
      721 cactatgcaa tcggacttca tgtatcaggc cgtgaaggcc gacgacgtcg acgtggtggc
      781 ggcctacact agcgacggcc gcatcgcgca attcgatctc gccgtgctcg atgaccccaa
      841 gcacgccatt ccgccctatg acgcaatcct gctgctggcg cccaaacgcg cgcatgatga
      901 ggccttgatt gccgcgctcc agccgctgat cggcgccgtt ccggtcgcgc tgatgcgcga
      961 ggccaatttg cgcgcctcca atggagccac gtctgccggc gaagtggcgc gctggctgga
     1021 cgatgagatc gcaaagaccc gctaggcgct agttgcggtc ttccttgcct tcgagctctt
     1081 cggcgcgtgc ttcagtcatg ctgcgcatgc attcgctggc gtcgatgcgg gcgatggtac
     1141 cctcgcccag cccgtaatag aggcaattgg cgtcgcggta ttggatccac aggcgctgcg
     1201 ccacgcgcaa ctgctcgcgc tgtttcggtt cgccggcatg aagcccgcgc tgataggcaa
     1261 tggccatgcg cttgtcccat tgcgcagcct tggcgttgag gcaatcgacc atttcgtagg
     1321 tgccgccgtc gcagcctttg tccggctcgc cttgatcgcc ggcaaatgca aacgcggcgg
     1381 ccccgatgag agcggccgct gcgatgacgg taatgcgcaa cacgcggatc aattcttgct
     1441 cttgtcgacc agcgcgcgct gcttgatcca cggcatcatc tcacgcagct tggcgccgac
     1501 ctgctcgatc ggatgctcag cgagcttggc gcgcgtcgcc ttgaacgagg tctgattgac
     1561 cttgttttcc agcatccagt cgcgcgtgaa gcggccggac tggatgtcgc cgaggatgcg
     1621 cttcatctcg gccttggtct cggcattgac gacgcgcggg ccggagacgt actcgccgta
     1681 ttccgccgtg ttcgacaccg agtaattcat gttggcgatg ccgccttcgt agatcaggtc
     1741 gacgatcagc ttcacttcat gcacgcactc gaaataggcc atctcgggcg cgtagccggc
     1801 ctcgaccagt gtttcgtaac cggccttcat caactccacg agtccgccgc acagcacggt
     1861 ctgttcgccg aacagatcgg tctcgcattc ttccttgaac gtggtctcga tgatgccggc
     1921 gcggccgccg ccgatcgccg aggcataaga caggccgaga tcgtgcgcgt tgccggaggc
     1981 gtccttatgg atcgccacca ggcacggtac gccggcgccg cgctgatatt ccgagcgcac
     2041 ggtgtggccg gggcctttcg gcgcgatcat caacacgtcg agatcggcgc gcggctcgat
     2101 cagattgaaa tgcacattga ggccgtgcgc gaacatcagc gcggcgccgt ctttcatgtt
     2161 cttggcgagc gactcgcgat aaatgtcgcc ctgcaattcg tcgggggtca gcatcatcat
     2221 cacgtcggcc catttggcgg cctcggcgac ttccatgacc ttgaacttct cggcctcggc
     2281 ctttttgacg ccggcggagg tcttgcgcag cgcgaccgcg acctccttga cgccgctgtc
     2341 gcgcaaattg agcgcgtggg cgtggccctg gctaccgtag ccgataatgg cgaccttctt
     2401 gcccttgatc aggttcaggt cggcgtcgcg atcgtagtaa acacgcatgt tacttacccc
     2461 tactcaggcc actcgttcgg gccgtttttc gttgccgtgt cgatagcaag acgttgatgc
     2521 ccgggacaag cccgggcgtg atgaaatcta gcgggccgac atcccctcgg gccctctcgc
     2581 aatagccgcg atgccggtgc gcgagacctc gaccaggccg agcggcagca tcagctcgac
     2641 gaactggtcg atcttgtcac ttttgccggt gatttcgaac acgaagctct cggtggtggc
     2701 a
//
LOCUS       DASWZT010000247         5153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_35713, whole genome shotgun sequence.
ACCESSION   DASWZT010000247 DASWZT010000000
VERSION     DASWZT010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35713"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="VGE92_06770"
     CDS             complement(<1..1060)
                     /locus_tag="VGE92_06770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HEY1313559.1"
                     /translation="MPGASHALGLGDIHVDSALNERLAAQIDIVGASADELDDLRAAV
                     ANRETFLRYGADRPSFLSSATFNVAHDTQGRPVLAIRSTEPFTEPLVNFLVDLRWRNG
                     ELIREYTLLLDPAGFASAVPAAPPAARPTVESKTQTVARQIEAPTVVRPDAEPSVDRS
                     GRKTTQIKVGPKATLRGIAWRIGERSPSDLQKMMIAIFRANPSAFDGNINRLYLGAVL
                     TIPSEAQLAAISADEAKREVHAQMTAWRETARPAVASASIAALPAATPSSAVTPLEAA
                     AAAAPLLSLPTAPASADPAAPAASTPVAASAAPTPAESAADEALDRRVHQLEQELGES
                     KGILESQNDQLLDLQQKAA"
     gene            1494..1901
                     /locus_tag="VGE92_06775"
     CDS             1494..1901
                     /locus_tag="VGE92_06775"
                     /inference="COORDINATES: protein motif:HMM:NF042861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbcA/ParS/Xre antitoxin family protein"
                     /protein_id="HEY1313560.1"
                     /translation="MNEANISIDYMSRASINTNPEPRRVLTGAVLRASGLLEITQAHL
                     AQILGLSPSTVSRMANGIYTLDTRKKEWELGALFVRLFRSLDAVIGSNDAAARSWLNG
                     QNLALLGRPIELIRSTEGLVRVVQYLDAARGRI"
     gene            1861..2571
                     /locus_tag="VGE92_06780"
     CDS             1861..2571
                     /locus_tag="VGE92_06780"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEY1313561.1"
                     /translation="MSSNIWTQRAVGSEARRARRRLWRAVEAQHIASTLRLVASVAEQ
                     LTLERLLEQSKPPALPAARDLHYLLSTPFRYTSPIGSRFRASADSGIWYGAEAQRTAC
                     AELGFWRWRFLIDSDALQSIGPSPQTVFRAGIDARMADLTQAPFKRSRADWTHRDNYG
                     PSQAFARVARQANVDAIRYESVRDPEHGAAVAVLTPACFKPRKPLERHTWFLTVRRTA
                     VIWQREGETFEFDARCWS"
     gene            2627..3673
                     /locus_tag="VGE92_06785"
     CDS             2627..3673
                     /locus_tag="VGE92_06785"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HEY1313562.1"
                     /translation="MSGNFKSDNVAPVCDAILAAIKAANAGPASAYGGDDLTLNLQCV
                     ASGIFGTEVAIFPVTTGTAANALALSQIVPPYAAVYCHEAAHIVTDECGAPEFFTGGA
                     KMIGLPAADGKITPEQVAGAIAFAEDMGVHHVKPGAISLSQATEWGTVYALSEIAALA
                     AVAKRHGLPVHMDGARFANALVHLGCTPAEATWKSGVDVLSFGATKNGALCAEAVVFF
                     DRERARDFERRRKRAGHLWSKLRFVSAQLLAHFENGLWLHNGGHANAMAAALAQGLQA
                     MPGARLLQAVNANEVFVALPEATVSGLERQGFGFYRWPLGGVSSGVAIRLVTSYATPR
                     AQVEEFLAAARQCT"
     gene            3664..4656
                     /locus_tag="VGE92_06790"
     CDS             3664..4656
                     /locus_tag="VGE92_06790"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HEY1313563.1"
                     /translation="MHLEIASESWPYKTPFRVSRGAEAALEVVVVTLSDADGNVGRGE
                     AAGVDYDGETVPLLAAQIEAIRPAIENGDRLALDASILPTLLPAGGARNAVDCALWDL
                     SAKRRRTTAWHLARIPNPRRLITCVTLGIDTDDEVAAGARRYSSWPLIKVKVDADHPL
                     DAPRIVHDICPAAGIIVDPNQAWSCDLLNRVAPALQALGVVLIEQPVPRGEDETLRGY
                     TGAVRLAADESVADRAGLPAVRALYDVVNIKLDKTGGLTEALALARDTASLGLGVMVG
                     CMAGTSLAMAPGMLVGQTAQFVDLDGPLLHAQDREHGIEYDRGLMSLPSSILWG"
BASE COUNT          908 a         1602 c         1697 g          946 t
ORIGIN      
        1 gagccgcttt ttgctgcaga tcgagcagtt gatcgttctg gctttccagg atgcccttcg
       61 actcaccgag ctcctgctcc agctgatgca ctctgcggtc gagcgcctcg tcggcggccg
      121 actccgccgg ggtcggcgcc gcgctcgcgg ccaccggtgt cgaagcggcc ggcgcggccg
      181 ggtctgccga cgcgggtgcg gtgggaagtg aaagcagcgg cgccgctgcg gccgcagctt
      241 caagcggcgt cacggcactc gaaggcgttg ccgcggggag ggcggcgatc gaggcggacg
      301 cgaccgcggg tcgcgcagtc tcgcgccagg cggtcatttg cgcgtgaact tcgcgcttgg
      361 cctcatccgc cgagatggct gccaactgcg cttccgaagg aatggttaga accgcgccaa
      421 gatacagacg attgatattg ccgtcgaatg cgctcgggtt cgcacgaaaa atcgcaatca
      481 tcattttctg cagatcggag ggcgagcgct cgccgatgcg ccacgcgatg ccgcgcagcg
      541 tcgctttcgg accgaccttg atctgtgtgg tcttgcggcc cgagcgatcg acgctcggct
      601 cggcgtcggg acgaacgacg gtcggcgcct cgatttgacg cgccaccgtc tgggtcttcg
      661 attcgaccgt cggccgcgca gcggggggcg cggctggaac tgccgatgcg aatcctgccg
      721 gatcgagcag caaggtgtat tcgcggatca gttctccgtt gcgccagcgc agatcaacca
      781 gaaaatttac caacggctcg gtgaacggct cggtggaacg aatggcaaga accggccgcc
      841 cttgcgtgtc gtgcgcgaca ttgaacgttg cggaggacaa gaatgacggt cgatcggcgc
      901 cgtagcgcaa gaatgtctcg cgattcgcga cagcggctcg caggtcgtcc aattcgtcgg
      961 cgctcgcgcc gacgatatcg atttgcgccg caaggcgctc attgagcgcc gaatcgacat
     1021 ggatgtctcc caagccaagt gcatgcgatg caccgggcat tgctaacgcc atcatcaaca
     1081 ttaacgggtt cttcccgctc gacatcgctt actccaaggt acggctttga acacctcgcc
     1141 acatggctgc aggcggtacc catcgcacac catggatcca ccttcaggca aatcctatgc
     1201 gacagaatct gggcttggat atagccgatt tccaagactt cgacgtagtt cacgcatgtt
     1261 gagcggcagc atcaaccgat cggcgaaaga ggcgccgttt cgaggcttca gtccggtccc
     1321 acatgtgacc cagttgctgt tcctggggag cgtcaaggac cggccgcgcc gcgccggttt
     1381 cgggtatttg cgcgaccttg aggggttcga cccgggcgtg agcagcagcg cagccgcggc
     1441 cgcaaggcgc atcttgcgaa tcatttgtcc gctttgcctt atttcagtag aatgtgaatg
     1501 aagcaaatat ttctatagat tacatgtcgc gcgcctcgat caacactaat cccgaacccc
     1561 gccgcgtact caccggcgcg gtgctgcgtg cctccggttt gctggaaatt acccaggccc
     1621 acttggcgca gattctcggt ttgagcccat cgaccgtgtc gcgcatggcg aatggaatct
     1681 acacgttgga cactcggaag aaggaatggg aactcggcgc cttgttcgtg cggctgttcc
     1741 gttcgctcga cgccgtgatc ggctccaacg atgcggcagc tcggagttgg ctcaatgggc
     1801 aaaatctggc gctgctcggc cggccgatcg aattgattcg cagcactgag ggcttggtcc
     1861 gtgtcgtcca atatttggac gcagcgcgcg gtcggatctg aggcgcggcg ggcgcggcgc
     1921 cgcctgtggc gcgccgtcga agcccagcac attgcctcga cgctgcgcct ggtggcgagc
     1981 gtcgcggagc aactgacgct cgagcgattg ctggagcaga gcaaaccgcc ggcgttgccg
     2041 gcagcgcgcg acctgcatta cctgctgtcg accccattcc gctacacctc acccatcggt
     2101 tcgcggtttc gcgcgagcgc cgattcgggg atctggtacg gcgccgaagc gcagcgcact
     2161 gcctgcgccg agttgggatt ctggcgctgg cggttcttga tcgacagcga cgcactgcaa
     2221 tccatcgggc cgtcgccgca gacagtgttt cgcgccggca tcgacgcccg tatggccgat
     2281 ctgacgcagg ctcctttcaa acgatcgcgc gccgattgga cgcaccgcga caactacggg
     2341 ccttcgcagg cgtttgcgcg ggtagcgcgg caggcgaatg tcgatgcgat tcgttacgag
     2401 tcggtccgcg atcccgagca tggagcggcg gtcgcggtgc tgacgcctgc gtgcttcaag
     2461 ccgcgcaagc ccttggaacg acacacctgg tttctgacgg tgcggcgcac cgcagtgatc
     2521 tggcagcgcg agggcgaaac cttcgagttc gacgcacggt gctggtctta atattcaagc
     2581 gcggcgcgca atgcgcttac aatcggcttt tcagcgaggg ggtctgttga gcggaaactt
     2641 caagagcgat aacgtggcgc cggtttgcga cgccatcttg gcggcgatca aagcggccaa
     2701 tgcgggcccg gcgagcgcct acggcggcga tgatctgacg ctcaacctgc aatgtgtggc
     2761 gagcggcata ttcggcaccg aggtggcaat cttcccggtc acgaccggaa ccgccgcgaa
     2821 cgcgttggct ctttcgcaga tcgtgcctcc atacgctgcc gtgtactgcc acgaggccgc
     2881 gcacatcgtg acggacgaat gcggcgcgcc ggaattcttt accggcggcg ccaaaatgat
     2941 cggacttccg gctgccgacg gtaaaattac gcccgagcaa gtggccggcg caatcgcctt
     3001 cgccgaggac atgggcgtgc accacgtcaa acccggcgcg atcagcctga gccaggccac
     3061 cgaatggggc accgtatacg cgctgagcga aatagccgcg ctcgctgcgg tggcgaagcg
     3121 gcacggattg ccggtacaca tggacggtgc ccgcttcgcc aatgcgctcg tgcatctggg
     3181 ttgcacgccg gccgaagcca cctggaaaag cggcgtcgat gtcctgtcct tcggcgccac
     3241 caagaacggt gcgctgtgcg ccgaggcggt ggtgtttttc gatcgcgagc gcgcccgcga
     3301 ctttgagcgc cggcgcaagc gggccggcca tctgtggtcg aagctgcgat tcgtgagcgc
     3361 gcagctgctc gcgcatttcg aaaacggttt gtggctccac aacggcggcc atgccaacgc
     3421 catggccgcc gctctggcgc agggcctgca agcaatgccc ggcgcacgcc tgttgcaagc
     3481 ggtgaacgcc aatgaggtat tcgtcgccct gcccgaagcg accgtgagcg gattggagcg
     3541 gcagggattt ggtttctacc gttggccgct cggtggggtc tcaagcggcg tggcgatccg
     3601 cttggtaact tcgtatgcga cgccgcgtgc acaggtggag gaatttctgg cggctgcgcg
     3661 gcagtgcact tagaaattgc gagcgagtcg tggccgtata agactccgtt tcgggtatcc
     3721 cgcggtgccg aagccgcgct cgaagtggtc gtcgtaacgc ttagcgacgc cgacggcaac
     3781 gtcggccgcg gcgaagccgc cggcgtcgat tacgacggcg aaaccgtgcc gctgctcgcc
     3841 gcgcagatcg aggcgatccg accggcgatt gagaacggtg atcggctcgc gctcgacgcg
     3901 tcgattctgc cgacgctatt gccggccggc ggagcccgca acgccgtcga ttgtgcgctg
     3961 tgggatttga gcgccaagcg ccgccgtacc accgcctggc atctggcgcg catcccgaat
     4021 ccacggcgcc tcatcacctg cgttacgttg ggaatcgaca cggatgatga ggtcgccgcc
     4081 ggcgcgcgtc gctattcatc gtggccgttg atcaaagtga aggtcgatgc cgaccatccg
     4141 ctcgatgcgc cgcgcatcgt gcatgacatc tgtcccgccg ccggaatcat cgtcgatccc
     4201 aatcaagcct ggtcgtgcga cctgctgaat cgagtggcgc ctgcgctcca ggcgctcggc
     4261 gtggtgctga tcgaacaacc ggtgccgcgc ggcgaagacg agacgctgcg cggctatacc
     4321 ggagccgtgc gcctggcggc cgacgagtcg gtcgccgatc gggcggggct gccggcggtt
     4381 cgcgcgttgt acgacgtcgt gaatatcaag ctcgacaaga ccgggggcct gaccgaggcg
     4441 ctcgcactgg cgcgcgacac ggcctcgctc ggactcggcg tcatggtggg atgcatggcc
     4501 ggcacgtcgt tggcgatggc gcccgggatg ttggtgggac aaacggcgca gttcgtcgac
     4561 ttggacggcc cgctgctgca cgcccaagac cgcgaacacg ggatcgaata cgaccggggt
     4621 ctcatgagct taccgtcgag cattctctgg ggctgaagcg gtgtggaccc gggtccaaaa
     4681 cccctcgcca cccccaggac ggtgtggcga gaagtccgtg actgttcgca acggtcgccg
     4741 cgcttggacg aggccgcggc acccgggagc gctgcgtgcc tcgctgtcgc accactataa
     4801 agatagcaat ttcagagatt taaggcgctt tccaaatgcg acgcgcagcg ttcggccgat
     4861 gccgtccagc gaaaaccctg cgccgcccga gcgcaactgc ggctgttgta gatttgacaa
     4921 aataggcgcc gcagattcat ataaggttag acaccaaaat gcggggatgg aattggatgg
     4981 acaattgacc agcaattcag agcccgttgg aacagtggat gttgcgctcg ctcacgcggc
     5041 gcgcttactc gatagggatg tcgcacttgc cgccgaacag gccggcgaga ttctcaaagc
     5101 cgtgccggga catccgcgcg cgcgcttgat tctcggtgcc tcgcaccggc tgg
//
LOCUS       DASWZT010000248         6928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_64772, whole genome shotgun sequence.
ACCESSION   DASWZT010000248 DASWZT010000000
VERSION     DASWZT010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6928
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64772"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VGE92_06795"
     CDS             complement(<1..199)
                     /locus_tag="VGE92_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313564.1"
                     /translation="MASALPMAAPGLSALDLLNIKSPVTGKPALNGKAKAHAAAQDFE
                     AVFLNSMFQHMFTGVDGEGPFG"
     gene            complement(199..1311)
                     /locus_tag="VGE92_06800"
     CDS             complement(199..1311)
                     /locus_tag="VGE92_06800"
                     /inference="COORDINATES: protein motif:HMM:NF003676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEY1313565.1"
                     /translation="MTRTLRALFLAGLLTAFASASAGAASRIKDLANIEGVRQNQLIG
                     YGLVVGLNGTGDTLNNIPFTKQSLTAMLERLGVNIRGQTIRTGNVAAVMVTANLPPFG
                     TQGTRIDVTVSALGDSKSLQGGVLLVTPLLGADGNVYAVGQGSVAIGGFQAEGEAAKI
                     VRGVPTVGRIANGALIEREIEFTLNRLSQLRMALRNPDFTTAKRIAASINDFMGTPTA
                     EPLDPGTVGITVPEQYRGNVISLLTEIEQLQVEPDQSAKIVIDERSGIIVMGRDVRVS
                     MVAVAQGNLTVTITETPQVSQPSPFSRGGSTRVVPRTTIGVQEDGKKLALVKDGVSLQ
                     QLVDGLNSLGIGPRDLIAILQAIKAAGAIQADIEVM"
     gene            1557..1967
                     /locus_tag="VGE92_06805"
     CDS             1557..1967
                     /locus_tag="VGE92_06805"
                     /inference="COORDINATES: protein motif:HMM:NF022227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliX"
                     /protein_id="HEY1313566.1"
                     /translation="MRIHGANGTALASAPATARRSAGGSFSISEQEQPRNSATAGALR
                     SISTVDALIALQGVENPMERKKRAVVKGRNALDVLDTLKLGLLDGSVDRSTLARLQVA
                     AEGLTEETGDPGLDAVLGEIDLRVAVELAKAGIR"
     gene            2200..2565
                     /gene="dksA"
                     /locus_tag="VGE92_06810"
     CDS             2200..2565
                     /gene="dksA"
                     /locus_tag="VGE92_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEY1313567.1"
                     /translation="MNERQRDYFRLKLLTWREDILKEAKETLLHLQEENQNHPDIADR
                     ASSETDRAIELRARDRQRKLIAKIDEALSRIDDGTYGYCEETGDPIALRRLEARPIAT
                     LSVEAQERHERRERVYRDD"
     gene            2694..4271
                     /locus_tag="VGE92_06815"
     CDS             2694..4271
                     /locus_tag="VGE92_06815"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1313568.1"
                     /translation="MPSHQTAHDASTLKRLLRKAHAAHVNGALAQAEKSYKAALDHDP
                     DNFEVLHRLGVLSHQRGRLSQALRFLAAAVKRNAGSAEVLSNHGLVLHALGRTDEAIA
                     SYEAALEKEPDNIDVLNKIGIALLDAGRPQEALTAFDRTLARAPRHVDALGNRGNAFL
                     KLNRPEQAISCYDTLRWIEGDTARLLTNRAHALRRLDRPEEALADLHKAVALDPNYAE
                     AQFELGIVQLTLGDFDRGWAAYERRWATAVFASHRRDFKSPLWTGEQSLNGRTILLHG
                     EQGLGDTIQFARYAAPVARLGATVILEVEPELAALMAGVEGPTRVIARGDKLAPFDLH
                     CPLMSLPRACKTSATNIPMDVPYIKIPEVKLASSTKRLPARRPLVGVCWAGRRSHHND
                     ANRSIALARFAPLFETEDVEFISLQRDVGAGDRALLRNRGNVLDIGDELRDFADTAAL
                     ISQLNLVVAVDTAVAHLAGALAKPLALLLPFAADFRWLRVRADSPWYPSATLFRQPRF
                     DDWESVIERVSLHMSNL"
     gene            complement(4436..5185)
                     /gene="flgH"
                     /locus_tag="VGE92_06820"
     CDS             complement(4436..5185)
                     /gene="flgH"
                     /locus_tag="VGE92_06820"
                     /inference="COORDINATES: protein motif:HMM:NF001305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEY1313569.1"
                     /translation="MNVLKVTRHAVLSAIAVSLLSSCAAIDRLKNAGQQPSLSSVDNP
                     TARPGYKPVQMPMPSAQPASYNPNSLWRNGSRAFFKDQRAHQIGDILTVQVNITDKAN
                     IANETQRSRTNTEDSGITNFLGSNTIKTPATAILPGRLLTADSTSSSDGKGSVNRSEA
                     LSTNVAAVVTQVLPNGNLVVEGKQEIRVNFEIRELIVAGIVRPEDIQSDNTIDSTKIA
                     QARIAYGGRGQITEVQQQRYGQQVMDVILPF"
     gene            complement(5277..6167)
                     /gene="flgA"
                     /locus_tag="VGE92_06825"
     CDS             complement(5277..6167)
                     /gene="flgA"
                     /locus_tag="VGE92_06825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HEY1313570.1"
                     /translation="MRIGDLIDHAGIVAKVPIFRAPDLGSTGSVSADAVVEAVRAYAL
                     VGLDTGDLSEVLVTRASRAIPAKDIEDRIAQALSTQYALGPAKDIAVNFDRELRALQL
                     EATAKGEPRVGYLNFDGRSGRFDVTLDIPTGATTRGTLRLSGRAAATVEVVTLTRPVE
                     RGEMIKSGDVVTERRPRAETDRDIVANADKAIGLAARASLQPGRPLRIADLMRPEQVQ
                     RNETVTLVYEVPGITLTVRGKAAEGGAQGDVIAVLNEQSKRTVQGVVDGPGRVIISTG
                     APRLVANLAPSKTASNDDPH"
     gene            complement(6294..>6928)
                     /gene="flgG"
                     /locus_tag="VGE92_06830"
     CDS             complement(6294..>6928)
                     /gene="flgG"
                     /locus_tag="VGE92_06830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HEY1313571.1"
                     /translation="TQTSAQGNMLPVGIDLGSGVKSVGTPRLMTQGTLAATGSTLDVA
                     IRGDGFFKIQLPDGTYAYTRDGSFQMDATGRIVTAQGNVVQPTITIPQNSSGLTINAQ
                     GQVSVIPQGSTTPQTLGQFTLTRFINQAGLLSLGDNLYQETPASGTPLDGLPSTDGAG
                     DLQQGNLEQSNVDSVTEITNLIAAQRAYEMNGKVITAADQMLQSDSNLMR"
BASE COUNT         1240 a         2092 c         2234 g         1362 t
ORIGIN      
        1 cgccgaatgg gccttcgccg tcgacaccgg tgaacatgtg ctggaacatg gaattgagga
       61 acacggcttc gaaatcctgc gccgccgcgt gcgccttggc tttgccattg agggcgggct
      121 tgcctgtaac cggtgatttg atgttgagaa ggtcgagcgc gctcaatccc ggcgcggcca
      181 tgggtagggc gctcgccatc acatcacctc gatgtcggcc tggatcgcgc ccgcagcctt
      241 gatcgcttgc aggatggcga tcagatcacg tggcccgatg ccgagtgagt tcaacccgtc
      301 gaccagctgt tgcagcgata cgccgtcctt gaccaatgcg agtttcttgc cgtcttcctg
      361 cacgccgatg gtggtgcgtg gcacgacccg cgtgctgccg ccgcgcgaga acggcgaggg
      421 ctggctgact tgcggcgtct cggtgatggt gacggtgaga ttgccttgcg ctaccgccac
      481 catcgacacg cgcacatcgc ggcccatgac gatgatgccg gagcgttcgt cgatcacgat
      541 cttggcagat tggtcgggct cgacctgaag ctgttcgatc tcggtgagca gcgaaatcac
      601 attgccgcga tattgctccg ggaccgtgat gccgactgtg ccggggtcga gcggctcggc
      661 ggtcggcgtg cccatgaagt cgttgatcga agcggcaatg cgcttggcgg tggtgaaatc
      721 cgggttgcgc aacgccatgc gcaactggct caggcgattg agcgtgaatt cgatctcgcg
      781 ctcgatcagc gcgccgttgg cgatgcggcc gacggttggc acgccgcgca caatcttggc
      841 ggcctcgccc tctgcctgga aaccgccgat cgccaccgag ccctggccga ccgcgtagac
      901 attgccgtcg gcgccgagca gcggggtgac cagcagcacg ccgccttgca ggctcttgct
      961 gtcgccgaga gccgatacag tcacgtcaat acgcgtgcct tgggtgccga acggcggcaa
     1021 attggcggtc accatcacgg cggcgacgtt gccggtgcgg atggtctggc cgcggatatt
     1081 gacgccgagg cgttccagca tcgcggtcag cgattgcttg gtgaagggaa tgttgttgag
     1141 ggtgtcgccg gtgccgttga gcccgaccac cagtccgtag ccgatgagct ggttctggcg
     1201 cacgccctcg atattggcga ggtccttgat acgcgatgcc gcgccggcgc tcgccgaagc
     1261 aaacgcggtc aatagcccgg ccagaaataa ggctcgcaac gtccgcgtca tcactctccc
     1321 caatgtgacg ttcgactgac acttgttatg cgaacgccgt gccaagccgg ccaagcgaat
     1381 caagactctg ggaatgctgg tgtttttgaa tcggagcgcg cggggcagcg cccttgcgga
     1441 ccggcaggaa acggccgagg cggcaagttt tgccggggct tttaccgggg attaaccata
     1501 aaagttcagg ctcacggtat tcggggaccg acaagctcgg ttagggtttt tcagcgatgc
     1561 gcattcatgg agcgaatgga actgcgctgg cgtccgcgcc ggccaccgcg cgccgcagcg
     1621 ccggcggctc cttcagcatc agcgagcagg agcagccgcg caattcggcc acggccggcg
     1681 cgttgcgctc gatctcgacc gtcgatgccc tgattgcgct gcaaggcgtg gaaaatccga
     1741 tggagcgcaa gaagcgcgcg gtggtcaaag gtcgcaacgc gctcgacgtg ctcgatacgc
     1801 tcaagcttgg tctgctcgac ggcagcgtcg accgctcgac actggcgcgc ctgcaggtgg
     1861 cggccgaggg actgaccgag gaaaccggcg atccgggtct agatgcggtc ctgggcgaga
     1921 tcgatctgcg ggtagcggtc gaattggcca aagcgggcat ccgctagcac tacgacagcc
     1981 ccctagcggt tccgtccaaa tagcctaagc cactgaaaca taacaatatt catgcagacg
     2041 agggaaccct actcggatat tgtccgcggg gcagcgcccg tatataagcc tgccgcaccg
     2101 ggagggtgga cttctcagct aataacaaaa gaagacaggg agttcagtgg gatgttggcc
     2161 aagaccaaga cgtatcggcc gtctgacaaa gagccgttta tgaatgagcg ccagcgcgac
     2221 tatttccgtc tgaagctgct cacctggcgc gaagacattc tcaaagaagc caaggagacg
     2281 ctcctgcatc tgcaggagga aaaccaaaat catcccgaca tcgccgaccg ggcttcctcg
     2341 gaaaccgacc gcgccatcga gctgcgcgcg cgcgaccgcc agcgcaagct gatcgccaag
     2401 atcgacgagg cgctgtcccg catcgacgat ggcacctacg gctattgcga agaaaccggc
     2461 gacccgatcg cgctgcggcg tctggaagcg cggccgatcg cgacgctgtc ggtggaagcg
     2521 caggaacgcc acgagcggcg cgaacgcgtg tatcgggacg actgagcacg tccccggcaa
     2581 tcgacaatta gcgaagctcc ctacaatgcc cggccgccgt gccgggcatt ttttcgttat
     2641 tgttccgcta acaccgtgat tcgggcctga tcggttgtga caacgctgcg gcgatgccct
     2701 ctcaccagac cgcccatgac gcttcaactc tcaagcgcct acttcggaag gcgcacgcgg
     2761 ctcatgttaa tggtgctctt gcgcaagcgg agaagtccta caaggcagct ttggatcacg
     2821 atccggataa tttcgaggtg ttgcatcgcc tcggcgtact cagccaccag cgcggtcggt
     2881 tgtcccaggc cctgcggttt ctggccgcag ccgtcaagcg caacgcagga tcggcggagg
     2941 ttctgtcgaa tcatggcctc gtcctgcatg ccctcggccg caccgatgaa gcgatcgcga
     3001 gttacgaagc cgcgctcgag aaagagccag acaatatcga cgtactcaac aaaatcggta
     3061 tcgcgcttct ggacgccgga cggccgcagg aggcgctcac ggccttcgat cgcacgctag
     3121 cgcgagcccc ccggcacgtt gatgcgctcg gcaaccgcgg taatgcgttt ctcaagctca
     3181 atcgtcccga gcaagcgata tcttgttacg acacgctgcg ctggatcgaa ggcgatactg
     3241 cgcggctcct caccaaccgc gcacacgcgt tgcgtcgact tgatcgcccc gaggaggctc
     3301 tggcagatct gcacaaggcc gttgcgttag accccaacta tgccgaagca caatttgagc
     3361 tcggcattgt gcaactcaca ctcggcgact tcgatcgcgg ttgggcggcc tacgagcggc
     3421 gttgggcaac cgcggtgttc gcgtcccacc ggcgcgattt caaatcgccg ttgtggaccg
     3481 gcgagcaatc gctgaacgga cgtacgattc ttttgcatgg ggagcagggc ttaggcgata
     3541 ccatccagtt tgcccgttac gccgcaccag tcgcgcgcct gggtgcaacc gtcatacttg
     3601 aggtggagcc tgagctcgct gctctgatgg cgggtgtgga gggtccgacg cgtgtcatcg
     3661 cacgcggcga caaacttgcc ccattcgatc tgcactgccc actcatgagt ctgccgcgtg
     3721 cctgcaagac cagcgcaaca aatataccaa tggatgtacc ttatatcaaa atccccgagg
     3781 ttaaacttgc atcgtcgacg aaacgcctgc ctgcgcgaag gcccctcgtc ggcgtgtgtt
     3841 gggcgggccg gcggtcgcat cataacgacg ctaatcgttc gatcgcgctg gcgcgattcg
     3901 cgccgctgtt cgaaaccgag gatgtcgagt ttataagttt acaacgcgac gtgggcgcgg
     3961 gcgaccgcgc gctgttgcgc aatcgcggca acgtcctaga cataggcgac gagttgcggg
     4021 actttgctga tacagcggcg ttgatttcgc aactcaacct tgtggtcgcg gtcgataccg
     4081 cagttgcgca tctcgccggc gcgctcgcca agccgttggc tctcctgctg cctttcgcag
     4141 ccgattttcg ctggctgcgc gtacgtgccg atagtccgtg gtatccgagc gcaacactgt
     4201 tcagacagcc gcggtttgac gattgggaga gcgtgattga gcgggtcagt ctgcacatgt
     4261 cgaatttgta gtcttctccg ccgggttcgg attcgacgga cgtcgcgacg gcaaaggctt
     4321 ccggcttgcc gaacgaacac ggcctccggc gggggagctg ccggaggccg cgtcgttgac
     4381 gccgtctacg ctcgcgcgat gcgaaaaacg gcgcgggagc ccgctatacc actgactaga
     4441 acggcaggat cacgtccatc acttgctggc cataacgctg ctgctgcact tcggtgattt
     4501 ggccgcgtcc gccataggcg atgcgggcct gcgcgatttt ggtggagtcg atggtgttgt
     4561 cgctctggat atcctccggg cgtacgatgc cggcgacaat caattcacga atttcgaaat
     4621 tgacgcggat ctcctgtttt ccttcgacca ccaaattgcc gtttggcagc acctgcgtca
     4681 ccaccgcggc gacgttggtc gacagcgctt cggagcggtt gaccgagcct ttgccgtcgc
     4741 tcgaggaggt cgagtcggcg gtcagcaggc ggcccggaag aattgccgtt gccggcgtct
     4801 tgatcgtatt gctgccaagg aaattggtga tgccggaatc ttcggtgttg gtccggctgc
     4861 gttgggtctc gttggcgata ttcgccttgt cggtgatgtt gacctggacg gtgaggatat
     4921 cgccgatctg atgtgcgcgc tgatccttga agaaggcgcg cgagccattg cgccacagtg
     4981 aattcggatt gtacgaagcc ggctgcgccg atggcatcgg catctgcact ggtttatagc
     5041 cgggccgcgc tgtcggattg tcgaccgacg agagcgaagg ttgctgaccg gcattcttca
     5101 gccggtcgat ggcggcacaa ctcgagagta gcgaaaccgc gatcgcggag agcaccgcgt
     5161 gacgcgttac tttgagaacg ttcatagaca tcactttcac aaatggcgcc acggcgccgt
     5221 ttcgtttcgg cattacttta gaactcgatg cacccgttac gcttcaactt catccttcag
     5281 tgcggatcgt cgttgctcgc ggtcttgctg ggcgcaagat tggcgaccag ccgcggtgcg
     5341 ccggtgctga tgatgacgcg gccgggacca tccaccacgc cctgcaccgt gcgcttggac
     5401 tgctcgttga gcacggcgat cacgtcgcct tgggcgccgc cttcagctgc cttgccgcgc
     5461 accgtgagcg tgatgcccgg tacctcgtag accagcgtca ccgtttcgtt gcgctgaacc
     5521 tgttccggcc gcatcaaatc ggcgatgcgc aacggccgtc ccggttgcag cgatgcgcgg
     5581 gcagcgagcc cgatcgcctt gtcggcattg gcgacgatat cgcggtcggt ctcggcgcgt
     5641 ggacggcgct cggtgacgac gtcgccactc ttgatcatct cgccgcgctc gaccggacgg
     5701 gtgagggtaa cgacctccac cgtggccgcc gcgcggccgg aaagccgcag ggtgccgcgc
     5761 gtcgtggcgc cggtcggaat atcgagcgtg acgtcgaagc gtccgctgcg gccgtcgaaa
     5821 ttcaggtagc cgacgcgcgg ttcgcctttg gcggtcgctt ccaactgcag ggcgcgcagc
     5881 tcgcggtcga aattcaccgc gatgtccttg gccgggccga gcgcgtattg tgtcgacagc
     5941 gcctgagcga tgcggtcttc gatatccttg gccgggatcg cgcgactcgc gcgcgtcacc
     6001 agcacctcgc tgaggtcacc ggtgtcaagc ccgaccagtg cgtaggcgcg caccgcttcg
     6061 accaccgcgt cggccgatac cgagccggtg gagccgaggt cgggcgcacg aaaaatcggc
     6121 accttggcga cgatgccggc atgatcgatc agatcgccga tgcgcacgat gtcgccggtg
     6181 acggtggctt ccgctctgag cttgggatgt tcctgatcgg ctgcggctgc gggggcgctt
     6241 gaggccgcga gggcggcggc aacgagtatg atattgcgga tcatgatttt gcctcagcgc
     6301 atcaggttgg aatctgactg caacatctgg tcggcagcgg tgatcacctt gccgttcatt
     6361 tcgtaggcgc gttgcgcggc aatcaaattt gttatttcgg tgaccgaatc gacgttggac
     6421 tgttcgaggt tgccttgctg caagtcgccg gcgccgtccg tgctgggtag gccgtcgagc
     6481 ggcgtgccgg aggccggcgt ctcctggtac aagttgtcgc caagcgacag cagacccgcc
     6541 tgattgatga agcgcgtcag cgtgaattga cccaaggtct gcggcgtggt cgatccttgc
     6601 gggatgacgg agacttggcc ttgcgcgttg atggtcaagc cgcttgagtt ctgtggaatc
     6661 gtgatggtgg gctgcaccac attgccctgc gccgtgacga tgcggccggt cgcgtccatc
     6721 tggaacgagc cgtcgcgtgt gtaggcgtag gtgccatccg gcaactgaat tttgaaaaag
     6781 ccgtcgccgc ggatcgcgac atcgagcgta ctgccggtcg ccgccaatgt gccttgggtc
     6841 atcagccgcg gcgtgccgac ggatttgacg cctgagccga gatcgatgcc cacggggagc
     6901 atgttgcctt gcgcggatgt ttgcgtgc
//
LOCUS       DASWZT010000249         8823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_55196, whole genome shotgun sequence.
ACCESSION   DASWZT010000249 DASWZT010000000
VERSION     DASWZT010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8823
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55196"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VGE92_06835"
     CDS             complement(<1..377)
                     /locus_tag="VGE92_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313572.1"
                     /translation="MVARRSGRKLTPAGLAHGIPIFLDQIIDMLAIEKRPDFVRSQES
                     NSDVGPMATLHGRDLLREGYSIEQVIRDYGDLCQAVTNLAVELGESISADEFRTFNRC
                     LDDAIAAAVTEYSQQNSQNALSY"
     gene            606..1337
                     /locus_tag="VGE92_06840"
     CDS             606..1337
                     /locus_tag="VGE92_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY1313573.1"
                     /translation="MVSPLQPSQNHLLGALPQPESQRWFPQLETVDLPLGAVIYESGV
                     TLSHVYFPTTAIVSLLYVMENGASAEIAVVGNEGIVGISLFMGGESTPSRAVVQSAGK
                     GLRLKASVMKEEFNRAGPVLHLMLRYTQALITQMAQTAVCNRHHSLDQQLCRWLLLSL
                     DRLQGNELVMTQELIANMLGVRREGVTEGALKLQLAGLISYTRGRITVLDRIGLEKRS
                     CECYAVVKKEYDRLLPPTAARTPGN"
     gene            complement(1356..1691)
                     /locus_tag="VGE92_06845"
     CDS             complement(1356..1691)
                     /locus_tag="VGE92_06845"
                     /inference="COORDINATES: protein motif:HMM:NF013517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HEY1313574.1"
                     /translation="MPAQITRRSVVKSGLVTSIAVAALGLTRTKASAAADLAALDPKD
                     PAAMTLGFINDSTKIDAATNPTHTAEQTCANCEQFLGKPSDARGGCVLFPGKSVPAAG
                     WCKVWRKKP"
     gene            complement(1726..2361)
                     /locus_tag="VGE92_06850"
     CDS             complement(1726..2361)
                     /locus_tag="VGE92_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY1313575.1"
                     /translation="MKLQHVLATALCAAIGLAGVAQSADPAVGIESDAQKAALRKTID
                     TCATCHGVEGRSVSPTFPNLAAQTAPYIELQLHAFKDQTRADPDAQAYMWGMASQLAD
                     ASISGLASYFSQQPAAQGRSGNAALVTQGKRIFVGGVAERQIPACATCHGAQAQGNGP
                     FPRLAGQHAPYLLKQLLVIQSVLRTAPVMHGVIKDLTRPQMEAVAAYLESI"
     gene            complement(2361..3764)
                     /locus_tag="VGE92_06855"
     CDS             complement(2361..3764)
                     /locus_tag="VGE92_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313576.1"
                     /translation="MTRFAIDYRRPFWVTLGLLVLCGVAAKPVWAVPSFARQTGMTCA
                     ACHTVFPELTPFGREFKLNGYVLDNIKQVTGIDTSARQTLAINSLPPISLMLQISYTH
                     TSAALPDSALKGALAKDGDLAFPQQASFFYAGKIADGLGAFIQITYDGIEDHFGFDNT
                     DVRYAHHLAFGGANGNSHDVIVGVTLNNNPTVQDVWNTTPAWGIPYSGSGTAPSPSTS
                     AKVDSGAGGFGQTVGGLGVYMWLDDHFYAEITDYTSAIPGGAHPLDSTQSNVVHGSAP
                     YWRLANEQRWDRNSLEIGVFGLSASTHPGNGTPLAGPPDKFRDIAADIQYQFIGEDHL
                     FTALATYISEHQTLQASVADGFAAEPDNKLKTLKLTGEYYYQRRIGGTVGYFSTTGSS
                     DALLYAPGPVTGSANNSPDSTGYILEVNYLPWLNTKLQAQYVGYEKFNGQKINYDGSG
                     RDASNNNTAYLLVWVNF"
     gene            complement(3959..4126)
                     /locus_tag="VGE92_06860"
     CDS             complement(3959..4126)
                     /locus_tag="VGE92_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313577.1"
                     /translation="MDLLIVLIVLLLLFGGGGGLYWGMGTGWGLGPIGLIIAALVIAY
                     LVYGYRGRRGI"
     gene            complement(4242..5705)
                     /gene="tldD"
                     /locus_tag="VGE92_06865"
     CDS             complement(4242..5705)
                     /gene="tldD"
                     /locus_tag="VGE92_06865"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF008006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEY1313578.1"
                     /translation="MSARSADLGEPMFDVAEKMILTPSGLDEGRLSTVLDAVMSRGVD
                     YADLYFQLSREESWSLEDGIVKEGSHNIEQGVGVRALSGEKTGFAYTDEVLLPALLEA
                     SDAARAIARSGASHAVKAWKRPLTHRLYLPVDPLDAIRDEDKVAWLMRIDADTRRIDP
                     RVKQVMASLTAVHEVVLIATSEGTLTADVRPLVRVNVSVIVEQNGRREQGYSGGGGRF
                     GLDELLRGDEPFAHGREAVRQALVNLEAVAAPAGSMTVVLGPGWPGILLHEAIGHGLE
                     GDFNRKGTSAFSNRIGQKVAADEITVVDDGTLDRRRGSLNVDDEGTPTQCTTLIEGGV
                     LRGYIQDKMNARLMGMPATGNGRRESFAHMTLPRMTNTYMRAGTRAPEEIIASVEKGL
                     YAVNFGGGQVDITSGKFVFSASEAYLIENGKLGAPVKGATLIGNGPDVLQRVSMVGTD
                     LKLDSGVGTCGKEGQSVPVGVGQPTLRIDALTVGGTG"
     gene            complement(5702..6541)
                     /locus_tag="VGE92_06870"
     CDS             complement(5702..6541)
                     /locus_tag="VGE92_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY1313579.1"
                     /translation="MGKVAAIQMTSGHIVADNLAAAGELLREAKDAGAEIACLPENFS
                     FIGLHDADKLQVAEVDGDGAVQAFLSATARKLKMWILGGTVVIRGDSERRVANASLLI
                     DAAGKRVARYDKIHLFDVTIPGRDEQYRESTHVTPGRAVVVADTPVGKLGLSVCYDMR
                     FPELYRDLVSQGAEWLAMPAAFTVPTGRAHWETLLRARAIENLCYVVAPAQTGTHSSG
                     RETYGDSLIIDYWGQVLSRLPKGSGVITADIDLAREAETRARFPVLDNRQLGLRAAAQ
                     GVR"
     gene            complement(6542..>8823)
                     /locus_tag="VGE92_06875"
     CDS             complement(6542..>8823)
                     /locus_tag="VGE92_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEY1313580.1"
                     /translation="YFPRALIPPPALVWLDRAFVGGHLSRADIVLQGPIKHFPFRDGS
                     GSFMARCALDGMTLDYGDGWPRLENLTGQAEFRNEGMTAHLVSGRVNDIPLESAEARF
                     ADFKTGELEVHVGASGDAGAALAFLRATPLDAAAEHAFSAVDARGPMQAQVDLFLPFK
                     DFVHRRVLVQGRLEGVSLNRTGSATSASDMNGDFEVDGAQVARADIRGRVLGGPFQMQ
                     ARTSRTRPLTRTQLEFRGSFNGDALRSALSLPGGIAIGGQTDWRGVLKMAPEPNRERS
                     LRLSSSLVGLDMQLPAPLDKAADIAMSSWLEIQWPASGGPQGRLSLGSVVSGSYALES
                     AADGLHLTRLALTFGGGETNPSDSQILNVGGSVARLDLAGWLKLNPADKNAKPLSNYL
                     RTAKLSVAELDYLGLAFRDVSLNLGAMEGGLRIAVGGPNVVGTITLPRAADSSEPWNL
                     QFERLQIDVPADEQDASAEPAPGNAQVLGDPRAIPAINFHAVQLNWGERKLGDVQATL
                     VKLDDGIRLQQLSVAGVSFNANATGEWRGKDAGVGRIMGTLTSSDVQDTVKQLGYVPV
                     IEAKSGRMDFDLNWVGAPTEEALSQAAGHVQLALDKGQVVGIKPGAGRVLGLTSVAAL
                     RRRLALDFSDLTDKGLAFDTVRGDLDLRDGSAYTENVLVKGPAAEIGLIGRVGLKNKD
                     YDQIAVVTGSVGNSLPLAALAGGPVVAGAVLLFTQVFKQPLKGLARGYYRITGSWDNP
                     IVERIKSTDAAAASAEAPK"
BASE COUNT         1638 a         2840 c         2622 g         1723 t
ORIGIN      
        1 ctgtaagata gggcgttctg gctgttctgt tgcgaatact cggtgacggc cgcggctatc
       61 gcatcgtcga ggcaacgatt gaaggtgcga aattcgtcgg cggaaatgga ttctcccagc
      121 tcaaccgcca gattggttac cgcttggcac agatcgccat agtcgcgaat gacctgttca
      181 atcgaatacc cctctcgaag caagtcgcgc ccgtgcaacg tcgccattgg gccgacatcg
      241 gagttcgatt cctgcgatcg cacgaaatca gggcgtttct cgatcgccaa catgtcgatg
      301 atttgatcca gaaagatcgg gatgccatgt gccagcccgg caggggtaag cttgcgtccg
      361 gaccttctcg ccaccatgac ctgacaccga tcgatcaaga ggagccggtg cgtatttagg
      421 agttcgtgca gcacacccca gaatagcttt gtcggccgag cgggtctgta cggcgcggca
      481 cgaacgccgc cgggcgcagc cccaatcggc cgtaggccgg atcttacgcc ggcgatgagc
      541 aaagcgtgtt atgttcgata ccgcaccgac gcagcaacta gaaacttcaa ttagggccaa
      601 aaagcatggt ttcgcctctc caaccgagcc aaaatcacct gttgggggcg ttaccgcagc
      661 ccgaatcaca gcgttggttt ccccagctgg agaccgtcga tttgccgttg ggggccgtaa
      721 tttacgagtc cggtgtcacc ttgagccacg tgtacttccc aaccaccgcg atcgtgtcgc
      781 tcctctatgt catggagaat ggtgcgtccg cggaaatcgc cgtcgtcggt aacgagggga
      841 tcgtcggcat ttccctattc atggggggtg aatccacgcc aagtcgagcc gtcgttcaaa
      901 gcgccggtaa gggactacgc ctgaaggcat ctgtcatgaa agaagagttc aatcgcgcgg
      961 gcccggtact tcacctcatg ctgcgctata cccaagcgct gatcacacag atggcgcaga
     1021 cggccgtctg caatcgccac cactcgctcg atcagcaact gtgccgctgg ctgttgctca
     1081 gcttagatcg cctgcagggg aacgaactgg tcatgaccca ggaactgatc gccaatatgc
     1141 tgggtgttcg ccgcgaaggc gtcaccgaag gcgcgctcaa attacagctt gcgggactga
     1201 tcagttacac gcgcggccgc atcacggtac ttgaccgcat tgggctcgag aaacgcagct
     1261 gcgagtgtta tgcagtggtc aaaaaggagt atgaccgact gcttcccccc acggcagcac
     1321 gcacgccggg aaactgacgt cttaccgggc gaatctcacg gcttttttcg ccaaactttg
     1381 caccagcctg ccgcaggcac gctcttcccg gggaatagga cgcatccgcc gcgagcgtcg
     1441 ctcggtttgc ccaaaaactg ctcgcaattg gcgcatgttt gttcggccgt gtgagttggg
     1501 ttggtcgcgg cgtcgatttt ggtcgaatca ttgatgaatc ccaacgtcat cgccgcggga
     1561 tctttcggat ccagcgcggc gagatccgcc gcggccgagg ctttggtgcg agtcaacccc
     1621 agcgctgcga ctgcaatgct cgtgacgagc ccgctcttga cgacgctacg acgtgtgatc
     1681 tgggctggca ttttgatctc ccggtgcgtg actcgaagcg ccgttctaaa tggactcgag
     1741 atacgcggcg acagcttcca tttgcggcct cgtcagatcc ttgatgaccc cgtgcatgac
     1801 cggcgcggtg cgcagcacac tttgaatcac cagcagttgc ttgagtaggt aaggtgcatg
     1861 ctgcccagcg aggcgtggga agggtccgtt gccttgcgcc tgcgcaccat ggcatgttgc
     1921 gcaggctgga atctgtcgct ccgctactcc ccccacaaat atccgtttgc cctgcgtgac
     1981 gagtgctgcg ttgccgctcc taccttgcgc ggcaggttgt tgagaaaaat agctggctag
     2041 tccgctgatc gatgcatcgg ccagctgtga ggccatgccc cacatgtagg cttgcgcatc
     2101 cggatcagcg cgtgtttggt ctttgaaggc atgcaattgc aattcgatat atggcgcggt
     2161 ctgtgcggcc agattaggaa atgtgggcga cacgctgcgg ccctcgacgc cgtggcacgt
     2221 ggcgcaagta tcgatcgtct tgcgtagtgc ggctttctgc gcgtcgcttt cgatcccgac
     2281 ggcgggatct gcactctggg caacccctgc gaggccgatc gcggcgcaca gggcggtggc
     2341 gagaacgtgc tgcaacttca tcagaagttc acccaaacca acagatacgc agtgttgttg
     2401 ttcgaggcgt cgcggccgct gccatcatag ttgatctttt ggccgttgaa cttctcgtac
     2461 cccacatact gtgcctgcag cttcgtattc agccagggca ggtaattcac ttcgagaatg
     2521 tagccggtgc tgtccgggct attgttcgcg ctgccggtga ccggcccggg cgcgtacagc
     2581 agcgcatcgc tgcttcccgt ggtgctgaaa taaccgacgg tgccgccaat gcggcgctgg
     2641 taataatatt cgcccgttaa cttgagtgtc ttcaatttgt tgtcgggctc ggctgcgaat
     2701 ccatcggcga cgctggcctg cagcgtttgg tgctcgctga tataggtcgc gagagccgtg
     2761 aacagatgat cctcgccgat gaattggtat tgaatatccg cggcgatgtc cctgaattta
     2821 tcgggcggac cggcgagcgg cgtgccgttt ccgggatgcg tgcttgcact caggccgaaa
     2881 acaccaatct ccaaggagtt gcggtcccag cgttgttcat tggcgagtcg ccagtaaggc
     2941 gccgaaccgt gcactacatt cgattgcgtg ctgtcgagag ggtgagcgcc cccgggtatc
     3001 gccgaggtgt aatcggtgat ctccgcataa aagtggtcat ccaaccacat gtagacaccg
     3061 agaccaccca cggtctggcc gaaaccgccg gcaccgctgt cgacctttgc tgaggtgctc
     3121 gggctgggcg ccgtgccgct tccggagtat ggaattcccc acgcgggtgt cgtgttccag
     3181 acgtcttgca ccgtgggatt attgttcagc gtgaccccca ctatcacatc gtggctattg
     3241 ccgttggcgc cgccaaatgc aaggtggtgc gcgtatcgaa catcggtgtt gtcgaatccg
     3301 aagtgatctt cgatgccgtc atacgtgatc tggatgaagg ctccaaggcc atcggcgatt
     3361 ttacccgcgt aaaagaagct cgcctgttgt ggaaaggcca agtcgccgtc tttcgcgaga
     3421 gctcctttga gcgcactgtc cggcagcgcc gcgctcgtat gcgtgtagga aatctgcaac
     3481 atcagactga tcggcggtag agaattgatt gccagcgtct gcctggccga ggtatcgatt
     3541 cccgtcacct gtttaatgtt gtcgagcacg tagccgttca acttgaattc ccgaccgaag
     3601 ggcgtgagtt cgggaaacac cgtgtggcag gccgcgcacg tcatgccggt ttggcgagcg
     3661 aagctgggta ccgcccagac aggcttggcc gcgactccgc agagcaccag tagcccgaga
     3721 gtcacccaaa acggacgtcg atagtcgatt gcaaagcggg tcatcgtttt tggtctccca
     3781 ctccgtctcg ccaagatcgt ccaacgtcca attcggtcgg aaaattaccg caggcaagtg
     3841 attcagtctg tgcggtaaca gacgtaggct cgccggcgtc gtcagacgcg ccgcccgtag
     3901 gcgcgaatct cgatgcgtct cagcgcgacc tcgatccggc catttcctca gggttgcgtc
     3961 atattccccg tctaccgcga tacccgtaga caaggtaggc gatcacaagg gcagcaatga
     4021 tcaggccgat cggaccgagc ccccaacccg tgcccatacc ccaatacaaa ccgccgccac
     4081 caccgaacaa cagcaacagc acgatcagaa caattagcag atccatgtct atctcctcgc
     4141 acgacaacgt catgtcgggg gatagtggct catggcgtat tgtcagtctg tacgctaacg
     4201 tacccgccgg gctcgcgccg cggccgtctc gcacttctcg atcagccggt gccgccgacc
     4261 gtgagcgcat cgatgcgcag cgtcggctgg ccgacgccga cggggaccga ttggccttcc
     4321 ttgccgcagg tcccgacccc gctgtcgagc ttgaggtccg tgcccaccat gctgacccgc
     4381 tgcaacacgt cggggccatt gccgatcagc gtcgcccctt tcacgggtgc accgagcttg
     4441 ccgttttcga tcaagtacgc ctcgctcgcg gagaacacga atttgcccga cgtaatgtcc
     4501 acttggccgc cgccgaaatt caccgcgtac aggcccttct cgaccgaggc aataatctct
     4561 tccggagcac gcgtgccggc ccgcatgtag gtattggtca tgcgcggcaa ggtcatgtgc
     4621 gcaaaggact cgcgccggcc gttgccggtc gccggcatgc ccatcaagcg cgcattcatc
     4681 ttgtcctgga tataaccgcg cagcacgccg ccttcgatga gcgtcgtgca ctgagtcggc
     4741 gtgccctcat cgtcgacatt caacgaaccg cgccgccggt ccaaggtccc gtcgtcgacc
     4801 accgtgattt cgtccgccgc cactttttgg ccgatccggt tcgaaaacgc ggaggtgcct
     4861 ttgcgattga aatccccttc cagcccgtga ccgatggcct cgtgcaacaa gatgccgggc
     4921 cagcccggac ccagcaccac ggtcatggat ccggcgggcg ctgccaccgc ctcgagattg
     4981 accagtgcct ggcgcaccgc ctcacgaccg tgcgcaaaag gctcatcgcc gcgcagcagt
     5041 tcatccaaac cgaagcgccc gccgccgccg ctgtaaccct gttcgcggcg gccgttctgt
     5101 tcgacgatga ccgagacatt gacgcgcacc agcggccgca cgtccgccgt cagcgtgccc
     5161 tcgctggtgg cgatcagcac cacctcatgg accgccgtga gcgatgccat cacctgcttg
     5221 acccgggggt cgatgcgccg ggtgtcggca tcgatgcgca tcagccacgc caccttgtct
     5281 tcgtcgcgga tggcgtcgag cggatcgacg ggcagataca accgatgagt gagcggacgc
     5341 ttccaggcct tgaccgcatg gctcgcgccg ctgcgcgcaa tggcgcgtgc cgcatcgctg
     5401 gcctcgagca gggccggcag cagcacctca tcggtgtacg cgaagccggt tttttcgccg
     5461 ctcaacgcac gcacgccgac accttgttcg atgttgtgcg acccctcctt cacgatgccg
     5521 tcctcgagcg accacgactc ttcgcgcgac agctgaaaat acagatcggc ataatcgaca
     5581 ccgcggctca tgacggcgtc gagcaccgtc gacaagcgac cctcgtcgag tcccgacggc
     5641 gtcaagatca ttttctcggc gacatcgaac atcggttcac ccaagtcggc actgcgcgcg
     5701 ctcatcggac cccctgggcg gctgcgcgca gtcccaactg ccgattgtcc aagacgggaa
     5761 agcgggctcg agtctcggcc tcgcgggcca agtcgatgtc cgccgttatc acgccgctgc
     5821 ccttcggcag gcgcgacagc acctgacccc aataatcgat gatcagcgaa tcgccgtagg
     5881 tctcgcggcc gctgctatgc gtgccggtct gcgccggcgc caccacataa cacaggtttt
     5941 caatcgcgcg tgcgcgcagc aacgtctccc agtgcgcgcg cccggtcggc accgtaaacg
     6001 cggccggcat cgccaaccat tcggcgcctt gcgacacgag gtcgcggtac agctccggaa
     6061 agcgcatgtc gtagcacacc gagagcccga gcttgcctac cggtgtatcc gcgaccacga
     6121 cggcgcggcc cggggtgacg tgggtcgatt cgcgatactg ttcgtcgcgc cccggaatgg
     6181 tcacgtcgaa caagtgaatc ttgtcgtagc gcgccacccg cttgccggcg gcatctatca
     6241 gcagcgaggc gttcgcgacg cgccgttcgc tgtcgccgcg aatcacaacg gtgccgccca
     6301 ggatccacat cttgagcttg cgcgcggtgg cgctcaagaa cgcttgcacg gcgccgtcgc
     6361 catccacttc ggcgacttgc agcttgtcgg catcgtgcag cccgatgaag gaaaaattct
     6421 cgggcaggca ggcaatctcg gctccggcgt ccttggcttc gcgaagcaac tcgccggccg
     6481 cggcgagatt atccgcgacg atgtggcctg aggtcatttg gatggcagca actttgccca
     6541 tttatttcgg cgcctccgca gacgctgcgg ccgcgtctgt gctcttgatt cgttcgacaa
     6601 tgggattatc ccagctgccg gtaatacgat aataccctcg tgccaggcct ttcagcggtt
     6661 gtttgaacac ttgtgtgaag agcagcacgg ccccggccac gaccggaccg cccgccaagg
     6721 ccgccaacgg cagcgaatta ccgacactgc ccgttaccac tgcaatttgg tcgtaatcct
     6781 tgtttttcaa gccaacccgc ccgataagtc cgatttccgc cgccggtccc ttgaccaaca
     6841 cgttctccgt gtacgcgctg ccatcgcgca agtccaaatc gcctcgaacg gtgtcgaacg
     6901 ccagcccctt gtcggtcaga tcgctgaaat cgagcgccag acgccggcgc agcgccgcga
     6961 cgctggtgag gccgagtacc cggcctgctc ccggcttgat gccgacgacc tggcccttgt
     7021 cgagggccaa ttgcacgtgg cccgccgcct ggctcaaggc ctcttcggtt ggtgcaccca
     7081 cccaattcag atcgaagtcc attcgaccgc tcttcgcttc gatcaccgga acatatccca
     7141 gctgcttcac cgtatcctgc acgtcgctgc tggtcagagt gcccatgatg cgtccgacgc
     7201 cggcgtcctt gccgcgccat tcgccggtgg cattggcgtt gaaactgacg ccggcgacgg
     7261 acagctgctg caaacggata ccgtcgtcga gtttgacgag ggtcgcttgc acgtcgccaa
     7321 gcttgcgctc gccccagttg agttgcacgg cgtgaaaatt gatggccggg atggcgcgcg
     7381 gatcgccgag cacctgcgca ttgccgggcg ccggctcggc gctcgcgtct tgctcgtccg
     7441 ccggcacatc gatctgcagg cgctcgaatt gtagattcca cggctcgctc gaatcggcgg
     7501 cgcgcggcaa ggttatggtc ccgaccacat tcggtccgcc gaccgcgatg cgcaagccac
     7561 cttccatcgc acccagattc aacgacacgt cgcgaaacgc gagacccaga tagtcgagtt
     7621 cggcaacgct cagcttcgcg gtgcgcaggt aattggacag cggcttggca ttcttgtcgg
     7681 cggggttgag cttgagccag cctgccagat ccagccgtgc gaccgatccg ccgacgttca
     7741 gaatttggct gtcactcggg ttcgtttcgc cgccgccgaa ggtcaacgcc aggcgcgtaa
     7801 gatgcaagcc atcggcggcc gattcgagcg catacgaacc gctcaccacc gagccgagcg
     7861 agagtcgccc ttgcgggccg ccgctcgccg gccattggat ctccagccag gaggacatgg
     7921 caatatcggc cgccttgtcc agcggtgcag gcagctgcat gtcgaggccc acgagactgc
     7981 tgctcaaacg cagactgcgt tcgcgattcg gctcgggcgc cattttcaaa acgccccgcc
     8041 agtcggtctg cccgccgatt gcaatgccgc caggcaggct tagcgcgctg cgtaatgcat
     8101 cgccgttgaa gctgccgcga aactcgagtt gcgtgcgcgt cagcggtcgg gtgcgcgagg
     8161 ttcgcgcctg catctgaaac ggaccgccca acacgcggcc gcgaatgtcg gcgcgcgcca
     8221 cttgcgcgcc gtcgacctcg aaatcgccgt tcatatcgct ggcagaggtg gcggatccgg
     8281 tccggttcaa gctcactccc tccaaccgac cttgcaccag cacgcgtcga tgcacgaaat
     8341 ccttgaacgg cagaaacaga tccacctgcg cttgcatcgg accgcgcgcg tcgaccgcgg
     8401 aaaaggcgtg ctctgccgct gcatccaacg gcgtcgcgcg caggaacgcg agcgcggcgc
     8461 cggcgtcgcc gctcgcgccc acatgcactt cgagttcgcc cgttttgaaa tcggcgaagc
     8521 gcgcctcggc cgattcgagt ggaatgtcgt tgacgcgtcc ggagacgagg tgcgccgtca
     8581 tcccctcgtt gcgaaattcc gcctgccccg tgagattctc gaggcgcggc cagccatcgc
     8641 cgtaatccag ggtcatgcca tcgagggcgc agcgcgccat gaacgagccg ctgccgtcgc
     8701 gaaacggaaa atgttttatc gggccctgca gcacgatgtc ggcgcgcgac aagtggccgc
     8761 caacgaaggc tcgatccagc cacacgagtg ccggcggcgg gatcaacgcg cgcggaaagt
     8821 aac
//
LOCUS       DASWZT010000250         4160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_31060, whole genome shotgun sequence.
ACCESSION   DASWZT010000250 DASWZT010000000
VERSION     DASWZT010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31060"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VGE92_06880"
     CDS             <1..194
                     /locus_tag="VGE92_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313581.1"
                     /translation="PKLPTRTVGVLMSLDPALGAVSGLLFLGEHLSWMQWAAIFGIMA
                     ASAGSAATSKAGTPQLLPD"
     gene            complement(180..881)
                     /locus_tag="VGE92_06885"
     CDS             complement(180..881)
                     /locus_tag="VGE92_06885"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEY1313582.1"
                     /translation="MLLVIIAAAAFLSTLAGGLLALKLHDKLHLVLGFSAGAVIGVAF
                     FDLLPEAISVGEKFHTPAFLLSGSALGFLSYLVLDRILAFHGDAAPRSRFAAAVLCIH
                     SLLDGIAIGFAFQASREVGLVVAIAVLTHDFSDGINTMSIVLKNRGERSQALRWLVAD
                     ALAPVFGIASTFFFTLPGAAFGAALALFAGFFLYIGASDLIPESYHAHPKFLTTAMTL
                     AGATVLYLAIVLIGQ"
     gene            927..2228
                     /locus_tag="VGE92_06890"
     CDS             927..2228
                     /locus_tag="VGE92_06890"
                     /inference="COORDINATES: protein motif:HMM:NF005455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HEY1313583.1"
                     /translation="MSYDSYHKRKIGNHVLKPETQMMGYGYDPSLSEGSLKPPIFLTS
                     TFVFKTAQDGKDFFDYTSGRREPAAGQTSGLVYSRFNNPNLEVLEDRLALWEEGEAAA
                     VFSSGMSAISTVIWTHVRPGDVILMSQPLYGGTETLIEKTLPAFGVNAISFTLGHDKD
                     AVSEAAATASARAAESGGRVCLIMVETPANPTNSLVDLKLMQDISVQIGARQRGGRPP
                     VAVDNTFLGPVFQRPLGLGADLVMYSLTKYVGGHSDLVAGGVVGSREAVSPVKRLRGA
                     LGTQLDPNTAWMIMRSMETLSLRMHKSADNAALVAKFLSAHPKVASVNYLGFLASDDP
                     RYGVFQRQCQSPGSTFGFAVKGGEAEAFRMLDALQVIKLAVSLGGTETLMSHPASTTH
                     SGVLKQTRDRIGVTDALIRISVGIEDADDLIADLAAALDAI"
     gene            2684..>4160
                     /locus_tag="VGE92_06895"
     CDS             2684..>4160
                     /locus_tag="VGE92_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1313584.1"
                     /translation="MPQFHCFSACRARAATSVVYLTCAFAPTFAAADAADSAADSSSS
                     GAAPPALDEIVVIAQHLNEARNGIQTQTGASTYTINEAAITAQPGGDNQLLNQVIMQA
                     PDVAQDSFGQFHVRGEHNGLQYRLNGIILPEGISVFGQSLDPRLISALTLITGALPAE
                     YGLRTAGIIDLKTKSGVIDPGGSVSLYGGSDGRLNYFVSGDLLRNDLGIESPDGRSSP
                     DHDQTTQYHEFAYLEDILDESNRLSAVLSSSVGKFEIPNRGNQVPGISNADGTPLEVD
                     GQTTFASDALNERQREVNHFAILSWQHSQGALDVQTSVTARYSSLNFVPDPLGDILFD
                     GIAQNAFKQNVAYALQSDAAYKLDDAHTVRAGVFLQTDHSKSLTTSAVIALNAAGNQI
                     SDIPENIVDNGSKTEWLESIYLQDEWKLIPTLTVNYGLRFDKFTAFTSGHQASPRVNA
                     VWQALDDTTVHAGYSRYLSPPPFELVGNETVSKFVNTTAAPL"
BASE COUNT          825 a         1286 c         1267 g          782 t
ORIGIN      
        1 tgccgaaatt gccgacgcgc accgtcggcg tactcatgag cttggatccg gcgctcggcg
       61 ccgtgtcggg cctgttgttt ctcggcgagc acttgagttg gatgcagtgg gcggcaatat
      121 tcggcatcat ggccgcctcc gccggcagcg cggcgaccag caaggccggc acaccgcagt
      181 tactgcccga ttagcacgat cgccaagtac agcaccgtcg cgccggccag agtcatggcc
      241 gtcgttagaa acttcggatg cgcatgatag ctctcgggga tcagatcgct ggcgccgatg
      301 tagagaaaga acccggcaaa cagcgccagc gccgcaccga acgccgcgcc cggcagcgtg
      361 aagaagaagg tcgaggcgat gccgaacacc ggagcgagcg catccgccac cagccaacgc
      421 agcgcctggg agcgctcgcc gcgatttttc aggacgatgc tcatggtgtt gatgccgtcc
      481 gagaaatcgt gggtgagcac cgcgatggcg accaccaggc cgacctcgcg cgaggcctgg
      541 aacgcaaagc cgatggcgat accgtccagc aggctgtgga tgcacagcac cgcggcggcg
      601 aatcggctgc gcggtgccgc atcgccgtga aacgccaaaa tccgatccaa gaccaggtag
      661 gacaggaagc cgagggcgct gccggacagc agaaacgccg gggtatgaaa tttctcgccg
      721 accgaaatcg cttccggcag caggtcgaag aaggcgacgc cgatgacggc gccggcgctg
      781 aaacccaaga ccaaatgcaa tttgtcgtgc agcttgagcg ccagcaggcc gcccgcgagc
      841 gtgctgagga aggccgccgc ggcaatgatt acgagcagca cggtgttata ttgcgcataa
      901 cgaatttgac tcggatgggg tgcagcgtga gttacgactc gtatcataaa cgcaaaatcg
      961 gcaatcacgt gctcaagcct gaaacccaga tgatgggata tggctatgat cccagcctct
     1021 cggaaggctc gctcaagccg ccgattttcc tgacctcgac gttcgtgttc aaaacggcgc
     1081 aggacggcaa ggattttttc gactacacct ccggacggcg cgagccggcg gcgggccaga
     1141 catcggggct ggtgtactcg cgcttcaaca acccgaatct cgaagtgctc gaggatcgcc
     1201 tcgcactgtg ggaagaaggc gaggcggccg cggtgttctc gagcggcatg tcggcgatat
     1261 ccacggtcat atggacgcac gtgcggccgg gcgatgtcat tctgatgagt cagccgctgt
     1321 acggcggaac ggaaacgctg atcgagaaga cgctgccggc attcggcgtg aatgcgatca
     1381 gctttacatt gggtcacgac aaggacgcgg tcagcgaggc ggccgcgacg gcatcggcgc
     1441 gcgccgccga atccggcggg cgggtgtgct tgatcatggt ggaaacgccc gcgaatccga
     1501 ccaacagcct ggtcgatctt aagttgatgc aggacatatc ggtgcaaatc ggcgcgcgcc
     1561 agaggggcgg ccggccgccg gtcgcggtcg acaacacgtt tctcggcccg gtttttcagc
     1621 gtcccttggg gctcggcgcg gacctcgtga tgtactcctt gaccaagtac gtgggcggac
     1681 attcggacct ggtcgcgggc ggagtcgtcg gctcgcgcga agcggtctct cctgtcaagc
     1741 gactgcgcgg tgcgctcggc acccagctcg atccgaacac cgcgtggatg atcatgcgtt
     1801 ccatggaaac cttgtcgctg cgcatgcaca agtcggcgga caacgcggcg ctggtcgcga
     1861 aattcctgag cgctcatccg aaggtcgcga gcgtgaacta cctggggttt ctggccagcg
     1921 atgacccgcg ctatggcgtg tttcagcgcc aatgccaaag ccccggttcg accttcggat
     1981 ttgcggtgaa gggcggcgaa gcggaggcgt ttcgcatgct cgacgcgttg caggtgatca
     2041 agcttgcggt gagtctcggc ggaacggaaa ccctgatgtc gcaccctgca agcaccacgc
     2101 attccggcgt gctcaagcag acccgcgatc gcatcggcgt caccgatgcg ctgatccgca
     2161 tttccgtcgg catcgaggac gccgacgact tgatcgcgga tttggccgcg gcgctcgatg
     2221 cgatctgaga tcggcagttc cctgttggta cggacggtgt tagcatctgc ctgtttctgt
     2281 gtgaagggtt gaccttgttc aaacggtcgc atcgcgtcga attccgcatc acggccttgg
     2341 ctgtgctggc gtttttcctt gcccaaatgg gcgcgatcgc gcatgcctac acgcacacgc
     2401 cgtcgaccca aacttccacc actcagcaaa gccatgaact gtgcggtgaa tgcttaaatt
     2461 ttgcgccgtt gctctctgca gccgcaacgc cggcgacgct gccgctgtcg ctgcaacgcg
     2521 gccgaagcgc acagcccgcg acgacgcccg attcttttct tgagcatcgt acccacctcg
     2581 cattccgatc ccgcgctccg cctatcgcac gctgactctc tttgagattc attgcgccgc
     2641 agattctctg tggcaccgcg cgtttaggag gagtacttga tccatgcccc aatttcattg
     2701 tttctcggcg tgccgcgcgc gcgccgcgac ttccgtcgtg tatttgacgt gcgcatttgc
     2761 gcccacgttt gcagcggccg atgccgccga tagtgccgca gactcgagca gctccggggc
     2821 tgcgccgccc gccctggatg agatcgtggt catcgcacag cacctcaacg aggctcgcaa
     2881 cggtattcag acacagacgg gcgcctcgac ctatacgatc aacgaagcgg ccattaccgc
     2941 gcagcccggc ggcgacaacc aactgctcaa tcaagtgatc atgcaggcgc ccgatgtggc
     3001 tcaggattcc ttcgggcaat ttcacgttcg tggcgagcac aacgggctgc agtaccgttt
     3061 gaacggcatc attctgccgg aaggcatcag cgtgttcggt cagtcgctgg atccgcgcct
     3121 gatttctgct ctgacgctca ttaccggcgc gctgccggcc gagtacgggc tgcgcaccgc
     3181 cggcatcatc gatctgaaaa ccaaaagcgg ggtaatcgat ccgggtggct cggtttccct
     3241 gtacggcggc agcgacggtc gactcaatta cttcgtctcc ggggacttgc tacgcaacga
     3301 tctgggcatc gagtcgccgg acgggcgctc cagccccgac cacgatcaga ccacgcaata
     3361 tcatgagttt gcttatttgg aagacattct cgatgaaagc aaccgtctaa gcgccgtgct
     3421 ttccagctcg gttggaaaat tcgagatccc gaatcgcggc aatcaagtgc cgggcatcag
     3481 caacgccgac ggtacgcccc ttgaggtcga tggccagacg acctttgcga gcgacgctct
     3541 caacgagcgg cagcgcgaag tgaaccactt tgccatcttg agttggcagc actcgcaagg
     3601 ggcgttggac gtacaaactt cggtgaccgc acgctattcc agcctcaatt tcgtgcccga
     3661 tccgctcggg gatattctct tcgacggcat cgctcagaat gcgttcaagc aaaacgtcgc
     3721 ttatgcgttg cagtccgacg cagcgtacaa gttggacgat gcacatacgg tgcgcgccgg
     3781 tgtgttcttg cagaccgatc actccaagag cttgaccacc tcggcggtca ttgcactgaa
     3841 cgccgccggg aatcagatca gcgacattcc agagaacatt gtcgacaacg gctctaaaac
     3901 cgaatggctc gagagcatct acctgcagga cgaatggaag ctcattccca cgctgaccgt
     3961 gaactatggg ctgcgcttcg acaagttcac cgcgttcacc tccggccacc aggcgagtcc
     4021 gcgggtcaat gccgtatggc aggcacttga cgacaccacg gtgcacgccg gctactcccg
     4081 ctatctatcg ccgccgccct tcgaactggt cggcaatgaa accgtctcga aatttgtgaa
     4141 taccacggct gcgccgctgt
//
LOCUS       DASWZT010000251         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_40719, whole genome shotgun sequence.
ACCESSION   DASWZT010000251 DASWZT010000000
VERSION     DASWZT010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40719"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1363
                     /locus_tag="VGE92_06900"
     CDS             143..1363
                     /locus_tag="VGE92_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HEY1313585.1"
                     /translation="MSRPLLEVADIFRDHGAAWRIANAGHVSLGQMKVMTAIERCRTA
                     ALGGHVGRCENQTCAHTIIAYNSCRNRNCPRCQGAAARQWLAEREAELLPVPYFHVVY
                     TLPAQIADIAYQNKAVIYDLLLKASAETTLTIAADPKHLGARIGFMSVLHTWGSALTH
                     HPHVHMVVPGGGLSPDGLKWIACRPRYFLTVEVLSALFRRLFLEMLVAAHHAGRLQFF
                     GDHARLADKAAFKAYLAPLRQIDWVVYAKEPFAGPRQVLRYLSRYTHRIAISNRRLVS
                     ADENGVTFKYKDYRIEGPARYKTMTLANDEFIRRFLIHVLPKGFHRIRHYGLLASSNR
                     AKNIAQARELLAVPAPAKQPETPEATLDQPRVQPQPCPCCGGRMIVIETFARGCEPRH
                     RPSPPPAAIRIDTS"
     gene            1867..>2220
                     /locus_tag="VGE92_06905"
     CDS             1867..>2220
                     /locus_tag="VGE92_06905"
                     /inference="COORDINATES: protein motif:HMM:NF024886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HEY1313586.1"
                     /translation="MSTEAISPLRQRMIEDMNARKLSAQTQRGHILSCRRFAAFLKRS
                     PDTATAENIRLFQLHLAETGMSICNRNRIMTGVRYLFRVTLRRLDLAAEIYHLREPQK
                     IAQVMSPDETRRLLAV"
BASE COUNT          427 a          764 c          645 g          384 t
ORIGIN      
        1 acacgacggc acgctacaca cgtgtcgcaa ccggcctgat cgccggtatc gagagcccgc
       61 ttgaccggct gtcgcggcct cgcaagaagc ccaggaaagc caaagagagg ggcagcaaag
      121 atcagtcgac cgcatagcgg ctatgtcccg tccattgctg gaggtcgcgg atatcttccg
      181 cgaccatgga gcggcgtggc gcatagccaa tgccggccat gtcagcctcg gccagatgaa
      241 ggtgatgact gcgatcgagc gctgccgtac ggcggcactc ggtggccatg tcgggcgctg
      301 cgagaaccag acgtgcgctc acaccatcat cgcctacaat agctgccgca atcggaactg
      361 ccccaggtgt cagggcgcgg ccgcacgcca gtggctggcc gaacgcgaag ccgagttgtt
      421 gccggtgccg tacttccatg tggtgtacac gctgccagcg cagatcgccg acatcgccta
      481 tcagaacaag gcggtgattt acgacctgct gctcaaagcc tcggccgaga ccacgctcac
      541 aatcgcggca gatcccaagc atctcggcgc gcgcatcggc ttcatgtcgg tgctgcacac
      601 ctggggttcc gcgctgacgc atcacccgca tgtgcatatg gtcgtgcccg gtggcggcct
      661 gtcgccggac ggattgaagt ggatcgcatg ccggccacgc tattttttga ccgtggaggt
      721 tctctcggcg ctgtttcgcc ggctgttcct ggagatgttg gtcgcggctc accatgccgg
      781 ccgcctgcag ttcttcggcg accacgcgcg gctcgccgac aaggctgcat tcaaggccta
      841 tctggcacca ctgcgccaga tcgattgggt ggtctacgcc aaggagccgt tcgccgggcc
      901 caggcaggtg ttgcgctatc tgtcgcgcta tacccaccgc atcgcgatct ctaatcgccg
      961 gctcgtatcc gccgacgaga acggcgtcac cttcaagtac aaggactatc gcatcgaggg
     1021 ccccgcccgc tataagacca tgacgttggc gaacgacgag ttcatccggc gtttcctgat
     1081 ccatgtgctg ccgaagggtt tccatcgcat ccgtcactat gggctgctcg ccagcagcaa
     1141 ccgcgccaaa aacatcgccc aagcgcgcga gctgctcgcc gtgccggctc ccgcaaaaca
     1201 gcccgagacg cccgaagcca cgctcgacca gccgcgcgtc cagccgcagc catgcccgtg
     1261 ctgtggcggc cgcatgatcg tcatcgagac tttcgcgcgc ggctgcgagc ccaggcaccg
     1321 gccttcgcca ccgcccgcgg caatcaggat cgacacatca tgatatcgtc gccagcgatc
     1381 caccaccgca gcaacatccg gcattcttgc tggcttccag ccggcaacgc ccaagctcac
     1441 atcggtacgc cggattcgcc cccattcgcg ccacctatct cgtcgcgctg cacccagatc
     1501 gctcgttcaa acccacccaa gcaaccatgg ctcgcggcaa cccgatcgcg gcaaccatct
     1561 caatccagcc tcagcaagcc aaaacccgtg gctaattccc catagcgccc gcggcaccgc
     1621 cgccacacac ctctcgcgat ttcgtgcctt ggcgcttttc ggccgccggc cgccggaacg
     1681 cgcggtcagg ccgtcatccc ggcagccgaa aacctgcaca aagcacgaaa tcgctgcgcg
     1741 atagcccgct gcgcgggtag cccgaccgcg agggcggcaa cccgaggaga gatggtcagt
     1801 agcgtgtggg tcatcctgtc ttgaagatcg aggtgtcgtc aacctcatcc aagacaggag
     1861 ttgcctatga gtacggaagc tatcagcccg ctgcgccagc gcatgatcga ggacatgaat
     1921 gcccgcaagc tctctgcgca aacgcagagg ggccacattc tcagctgcag gcggttcgct
     1981 gcatttctaa agcggtcccc cgacacggct acggccgaga acattcgcct gttccagttg
     2041 cacctggccg agacgggcat gagtatttgc aatcgcaatc gcatcatgac tggagtgcga
     2101 tacttgttcc gcgtgacgct gcggcggctg gatcttgccg ccgagatcta tcacctccgc
     2161 gaaccccaga agatagcgca ggtgatgagc ccggacgaga cccgtcgcct gctggccgtc
//
LOCUS       DASWZT010000252         1649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_59962, whole genome shotgun sequence.
ACCESSION   DASWZT010000252 DASWZT010000000
VERSION     DASWZT010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59962"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /locus_tag="VGE92_06910"
     CDS             <1..1222
                     /locus_tag="VGE92_06910"
                     /inference="COORDINATES: protein motif:HMM:NF037277.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313587.1"
                     /translation="DMGLTFYMTLSLGAFLLAQQAPQSRPWMMLAWAATAGGVLSKGI
                     VAAAIPAAVLILYSIYSRDVAPWRRLHLKWGLALFLCITVPWHWLAARRLADFLEFFF
                     VHEHLARYLTPSADREEPWWFFVPVFLAGSLPWTLSALRSLMGGWRRPARYAPFNPVL
                     FLWIWIVFIGVFFSLSDSKLMSYLLPAMPALAMLVAARPADTLQRDFRYTAAITVLLG
                     LSLAVASLNWPRVIATSDRSAVFLPLAKPLGRIALLLMVSGAFVLVQRVRGATRDAVF
                     LGAGWCLAWLLLIRAAAAAAPIYSGANLAAALPVGDRDAPLYSVATYDQSLPFYLRRT
                     LTLVQYRGELEYGLRQAPGADIPDVADFLRLWSSQSRAFAVMERDMFDDFKRRGVPMR
                     LVAQNVNRVLVSRQ"
     gene            1219..1599
                     /locus_tag="VGE92_06915"
     CDS             1219..1599
                     /locus_tag="VGE92_06915"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HEY1313588.1"
                     /translation="MSWVTWVLILTGVGLNAAAQLLLKVATRPLAHFSQFNLATLGGS
                     VAILLQSLPFWTGMLCYGASVCVWLAALSKAPVSTAYPMLSLGYVVVAAVSVLWLGES
                     MTPAKVLGIALICAGVILVSRSSV"
BASE COUNT          228 a          494 c          541 g          386 t
ORIGIN      
        1 ggacatgggt ctgacgtttt acatgacctt gagtctcggc gcattcctgc tggcgcagca
       61 agcgccgcag tcccgcccct ggatgatgct ggcatgggcc gcaaccgccg gcggtgtgct
      121 gagcaagggc atcgtcgcgg cggcgatccc ggccgcagtg ttgatcttgt acagcatcta
      181 ttcgcgcgac gtcgcacctt ggcgcaggct gcacctcaag tggggcctgg cgctgttttt
      241 gtgcatcacg gtaccttggc attggctcgc agcgcgtcgc ctggccgatt ttctagaatt
      301 ctttttcgtc cacgagcatc tggcgcgcta cctgacaccg agtgccgacc gcgaggagcc
      361 ctggtggttt ttcgttcccg tattcctggc gggcagcctg ccgtggacgc tgtccgcatt
      421 gcggtccctg atgggcggct ggcggcggcc tgcaaggtac gcgccattca acccggtctt
      481 gtttctatgg atttggatcg tgttcatcgg cgtgtttttc tcactctccg attcgaaact
      541 catgagctac ctgttgccgg cgatgccggc gctggcgatg ctcgttgccg cgcggccggc
      601 ggatacgttg cagcgtgatt ttcgctatac cgcggcaatc accgtgctgc tgggattgag
      661 tttggcggtc gccagcctca attggccccg cgtcatcgca acctccgaca ggagcgcggt
      721 cttcctgccg ctggcgaagc cgttgggccg gatcgcgctg ctgctcatgg tctcgggtgc
      781 tttcgtactc gtccagcgtg tgcgcggggc gactcgcgac gcagtatttc tcggggcggg
      841 ctggtgtctt gcatggctgc tgctgatccg cgccgccgct gcggcggcac cgatctactc
      901 cggggcgaat ctcgcggctg cgcttccagt cggcgaccgc gatgcgccgc tgtacagcgt
      961 cgccacctac gatcaatctc tgccgttcta tctgcgccgc acgcttacgc tggtgcagta
     1021 tcgcggcgag cttgaatacg gattacgcca ggccccgggc gccgacattc ccgacgtggc
     1081 ggatttcctt cgcctctggt cgtcgcaatc ccgcgccttt gcggtgatgg agagagacat
     1141 gttcgatgat ttcaagcggc gcggagtacc gatgcgattg gtggcgcaaa acgtcaaccg
     1201 cgtcttggtg tcgcggcaat gagttgggtt acctgggtgt tgatcttgac aggggtggga
     1261 ttgaacgccg ccgctcaatt gctgctcaaa gttgcgactc ggccgctcgc gcatttctcg
     1321 cagttcaatc tcgcaacgct cggcggcagc gtcgcgattc tgctgcagag cctgccgttc
     1381 tggaccggga tgctgtgcta tggcgcaagc gtatgcgtgt ggctcgcggc gctgtccaag
     1441 gcgccggtca gcactgcgta tccgatgcta tccttgggct acgtggtggt cgccgcggtg
     1501 tcggttctat ggctgggcga gagcatgacg cctgccaagg tgcttggaat cgcgctcatc
     1561 tgtgccggtg tgattctggt ttcgcgcagc tcggtatgaa ggcatctttc gcggttgcga
     1621 ccgaaaatag gatggcgcag actggcggg
//
LOCUS       DASWZT010000253         3628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_50180, whole genome shotgun sequence.
ACCESSION   DASWZT010000253 DASWZT010000000
VERSION     DASWZT010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50180"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VGE92_06920"
     CDS             complement(<1..405)
                     /locus_tag="VGE92_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313589.1"
                     /translation="MFGHARSRDLAFFSLLLLVVAARADDTSYDPRSTAAAPCGVGTP
                     APGHAPPELRPAADGLTLTVRQDGSRLCYVANGSADAPIIHVRLGADLAVTLQNEITD
                     PAAIDAVSGPAKLNTPNEPVPLAAGFYRVIPGM"
     gene            complement(424..1701)
                     /locus_tag="VGE92_06925"
     CDS             complement(424..1701)
                     /locus_tag="VGE92_06925"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY1313590.1"
                     /translation="MRKRILAMSMALVAAGGASAAQGPVPRGIPPLEHVFVIMMENHA
                     YGQIAGNPQAPFLNSLMKKENLATNYFAIAHPSNTNYLEVVGGSNFNKLSDQYPDWHN
                     ANCVPSIVPGQPTNTDSPNLGVFCPISGKNGVDAAIPVLDTSTNETSAPPLTNIDGKR
                     SLPAGTRIDGISIADQLVAAEKSWKSYQEGLPLIGADGVNVSDGYYAIGGKNAEGINV
                     TSDFAALSTAGNPVSGSDVVFLYAVKHNPFVYFASVQEGSDRALSLERTVSFDGPKGL
                     YADLKAGTVPDFAFIAPNQCDDMHGRGNGTAFCNYDADDNGTQTGLNPSLILQGDLSV
                     QRIVTAIKASPVWSNSRSAIVILWDENDYSTITSNQVVLIVDTNYGPHHLVSQKYYDH
                     YSLTKTLDAAFRLPCLNHACDAGVEVMSDLFRE"
     gene            2077..>3628
                     /locus_tag="VGE92_06930"
     CDS             2077..>3628
                     /locus_tag="VGE92_06930"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEY1313591.1"
                     /translation="MPLKDLALRNRAAPVQWVATATALSGLMSLCGWAFHFPALTSIL
                     PGSVEMKANTAISLILCGISEIILVDRAGARLDRFAQVLAVIALAIGLATLAEYVFGW
                     QLGIDQLLFDDTAGAYNVFRGRMSPFSAAAFVALGIALVAKPYESLSRLSQWSAALVL
                     LIGSTSLIGYLWNAGELITDRWLPPLALNTAVCLLLLGIGALFAPSRTAKERTRPRTE
                     LASVEIKILAGFLLAMTLLLFGGSFTYRASVEFASSVDWIAHTQAVRTTLADLRGSLT
                     GAQLAQRDYLLTSEQSRWDEYLQHIDEVQSRLAALQRLTADNPVQRENLAFLRSAVDA
                     RMTEMAAGVTALNSFGIPAARAVLRILRLNRNGADVQTAFERMQLVEAALLARREAVS
                     LRVRQTTLISLLITLLAAAVLFIALFRGIHREMRARRGAEDALRASEQYNRSIVESSP
                     DCLAILTLDARVSQMAPQGLRLMGIDDFSTINDSDWLALWRGDDSSAALLAVEAARAG
                     SAGRFRASC"
BASE COUNT          664 a         1123 c         1101 g          740 t
ORIGIN      
        1 catgccgggt atgacccgat agaagccggc agcgagcggc acgggctcat ttggggtgtt
       61 gagttttgcg ggcccgctga cggcatcgat cgccgccggg tccgtgattt cattctgcag
      121 cgtaaccgcc agatccgcgc ccaggcgcac gtggatgatg ggcgcgtcgg cgctgccgtt
      181 ggcaacgtag cagaggcggc tgccatcctg gcgaaccgtg agcgtcaaac catccgcggc
      241 cgggcgtagt tccggaggcg catgacccgg ggccggtgtg ccgacgccgc acggggccgc
      301 tgcggtactg cgcgggtcgt acgaggtgtc gtcggcccgc gctgcgacca ccagaagcag
      361 caacgagaaa aacgcgagat cgcgtgacct cgcgtggccg aacatcgggt cagcagcgac
      421 ttcttattcc cgaaacaggt ctgacatgac ttccacgccc gcatcgcacg cgtgattcaa
      481 acacggaagt ctgaacgccg catcgagcgt cttggtcaac gagtaatggt cgtagtactt
      541 ctggctcacc aagtggtgcg ggccgtaatt cgtatcgacg atcagcacca cttgattcga
      601 cgtgatcgtg ctgtagtcat tctcatccca aagaatcacg atcgcgctgc ggctgttcga
      661 ccacacaggc gaggctttga tcgccgtgac gatgcgctgc acggagaggt cgccctgcag
      721 gatcaaagag gggttgagtc cggtctgagt gccattgtca tcggcgtcat aattacagaa
      781 cgccgtgcca ttgccgcgcc cgtgcatgtc gtcgcactga ttgggcgcga tgaaggcgaa
      841 atccggcacc gttccggcct taagatccgc atacagaccc ttcggcccat cgaacgagac
      901 cgtgcgctcg agactcagcg cgcgatcgct gccctcctgg acggaggcga aatagacgaa
      961 cggattgtgc ttcacggcat acaggaagac cacatccgag ccgctcaccg ggttcccggc
     1021 ggtggagagc gctgcaaagt ccgaggtgac gttgatgcct tccgcgtttt tgccgccgat
     1081 cgcgtagtag ccgtcgctga cattcacgcc gtccgccccg atcagcggca gaccctcctg
     1141 gtaggacttc caagacttct cggccgcgac caactgatcc gcgatggaga taccgtcgat
     1201 cctcgtccct gccggtagcg agcgcttgcc gtcgatgttc gtcaggggcg gcgccgacgt
     1261 ctcgttggtg ctcgtgtcga gcaccgggat ggccgcgtcg acaccgttct tgcccgagat
     1321 cgggcagaac acacccaaat tcggcgagtc ggtattcgtc ggttgtcccg gcacgatgct
     1381 cggcacgcag ttggcgttgt gccagtccgg atattgatcg gacagcttgt tgaagttgga
     1441 gccgccgacc acctccagat aattggtatt gctcggatga gcaatcgcaa agtaattggt
     1501 cgccagattt tcctttttca tgagcgaatt gaggaagggt gcctgcggat tgccggcgat
     1561 ctgtccgtag gcgtggtttt ccatcatgat cacaaacacg tgctcgagcg gcggaatgcc
     1621 ccgcggaacc ggaccttggg ctgcactcgc gccgcccgcg gccaccaaag ccatgctcat
     1681 tgcaaggatt ctcttcctca tcagatcgct ccccgatttt tggatttaga cggtgcgcag
     1741 tggcatcgct ttgaggcaac gcacaacgcg ggcttcgccg gatcgtaggg gggaactgtg
     1801 acagaacggt gtatccgcca tgacatgcgg gccccgacga gcgctcgcaa ttccacggcc
     1861 aagtagctga tattatggta aatacgattc gcgcaccccg aagtctcgaa cccagaaaac
     1921 acccaactgc caagatgggc tttcccgcta gccgtctgag gggtttttcc gcttcccaga
     1981 accaggcgag ttcggtctga tcgtcaggta ttgcgccgcg ctaaatcctt cagcaaccgt
     2041 gctcaaagcc gatacgaggg gaggatcaaa cgccaaatgc cacttaaaga cctcgctttg
     2101 cgcaatcgcg ccgcgcctgt gcaatgggta gccacggcca cggcattgtc cgggttgatg
     2161 agtctgtgcg gatgggcgtt ccatttcccg gcgctgacca gcatcctccc tggttcggtg
     2221 gaaatgaaag ccaacaccgc aatctcgctc atcctctgcg ggatatcgga aatcatcctc
     2281 gtggatcggg ccggtgcacg actagatcga ttcgcccagg ttctcgctgt gatcgccttg
     2341 gctatcggcc tggccacact ggccgagtac gtattcggtt ggcagctcgg catcgaccag
     2401 ttgctcttcg atgataccgc cggtgcttac aacgtgttcc gcggtcgcat gtcgccgttc
     2461 agtgcggcgg ccttcgtcgc gctcggcatt gcgctcgtcg ccaagccgta tgagtcgctc
     2521 agtcggcttt cccaatggtc ggcagccttg gttctactca tcggatcgac ttccctcatc
     2581 ggctatcttt ggaatgcggg agaactgatt accgatcgtt ggctgccgcc gctggcgctc
     2641 aacacggcgg tctgtctgtt attgctcggc atcggcgcgc tgttcgcgcc cagccggact
     2701 gccaaggaac ggacccggcc gcgcaccgaa cttgcatccg tcgaaatcaa aatcttggca
     2761 ggctttctgc tcgccatgac cctgctgctg ttcggcggca gctttactta ccgtgcaagc
     2821 gtcgagttcg ccagctcggt cgattggatc gctcatacgc aggcagtgcg cacaactctc
     2881 gccgatcttc gcggttcact gacgggcgcg caactggcgc aacgcgatta cttgctcacc
     2941 tccgaacaat cgcgctggga tgagtatctg cagcacatcg atgaggtgca atcgcgcctg
     3001 gcggcgctgc agcgattgac agccgacaat ccggtgcagc gcgagaacct ggccttcttg
     3061 cgttccgccg tcgatgcgcg catgaccgaa atggccgcgg gcgtcaccgc gctgaactcg
     3121 tttggtattc cggccgcgcg cgccgtgctg cggattttac gtctcaacag aaacggcgcg
     3181 gacgtgcaaa ctgcattcga acgcatgcag ttggtcgaag cagcccttct cgcaaggcgc
     3241 gaagcggtgt cgttgcgcgt tcgacaaacc acgctcattt cgctgctgat caccttgctc
     3301 gccgcggcgg tgctgttcat cgcactgttc cgagggattc atcgcgaaat gcgcgcgcgg
     3361 cgcggcgcag aggacgcgct tcgcgccagc gagcagtaca accgcagcat cgtggagagt
     3421 tcgccggact gcctggcaat cttgactctg gatgcgcgcg tcagccagat ggcgccgcag
     3481 ggtctgcgcc tgatgggcat cgacgatttc tcgaccatca acgacagcga ctggctggca
     3541 ctctggcgcg gcgatgattc atcggccgct ctgctcgccg tcgaagccgc tcgcgccggc
     3601 agcgccgggc gattccgcgc ttcatgcg
//
LOCUS       DASWZT010000254         2090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_55244, whole genome shotgun sequence.
ACCESSION   DASWZT010000254 DASWZT010000000
VERSION     DASWZT010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55244"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VGE92_06935"
     CDS             complement(<1..399)
                     /locus_tag="VGE92_06935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY1313592.1"
                     /translation="MNAEHHRPLPAYLEQAQPLQAAVRSGPELSIIVPTFNERENIAE
                     LIGRLVLCMGSRSWEVVFVDDDSPDGTAHVVREHATEDSRVRCVQRIGRRGLSSACIE
                     GMLATTAPFIAVMDADLQHDEKLLPQMLDTL"
     gene            605..>2090
                     /locus_tag="VGE92_06940"
     CDS             605..>2090
                     /locus_tag="VGE92_06940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY1313593.1"
                     /translation="MGLFCALIGQGASLRSSHIASFAVAMALNYLLKVRSTVVARGRG
                     GDLRLHGRLIILSLMALFLRGGVLGLLAQDWGWRPQVAIVFAVLAGLTVTLPGYAAVL
                     SAAQGNARARALAVGLIAYGLALRLVYSGSVELLPEEAYYWNYSRHLDIGYLDHPPMV
                     AWLIRLGTALFGQSEFGVRAPALGCAAIASVFTYRLTRNLFGEPSALAALLLAQVLPY
                     FFLSGLLMTPDAPLVAAWAASLYYLERALVAGLIGSWWRAGLALGIGGLSKYSIGLLV
                     PVTILFMLWDPRARSNWRRWQPYAAALLALAVFSPVIVWNAQHDWASFVFQTSRRLAE
                     APRFALHKLLASALILITPTGVLAIAAAFTGARPDNGPAGDMRRRWLFLGIAIGLPLA
                     VFAIFSLRHEVKLDWTGAPWLAALPAMAAGMSGVETSTRGFAARVGAAWLPTAVTLLL
                     IYGAGLHYLVLGLPGLGYGAHIELVPVAWRDLSRRVAAATQAIHQ"
BASE COUNT          311 a          684 c          648 g          447 t
ORIGIN      
        1 caaagtgtcg agcatctgcg gcagcaattt ctcgtcgtgc tgcagatccg cgtccatcac
       61 cgcgataaaa ggtgccgtgg tcgcgagcat gccctcgata caagccgaag ataggccgcg
      121 ccggccgatt ctctgcacac accgcacccg actgtcctcc gtcgcgtgtt cgcgcaccac
      181 atgggcggtg ccgtcgggcg aatcatcgtc tacgaatacg acttcccacg atcgagatcc
      241 catgcacagc accagccgcc cgatcagctc cgcaatattt tctcgctcat tgaacgtcgg
      301 cacgatgatc gacaattccg gtccggaacg caccgctgcc tgaaggggct gcgcttgctc
      361 cagataggca gggagcgggc gatggtgttc agcgttcatc taatctgtcg tctcccggat
      421 gcgtcttttt cgcccagcgt cgaatcgtta tgttgttact ataatcaccc cgcggcgccg
      481 gcgcacgaat tcatgaagct ttcgcccccc gtcgtaacca tggtttcgaa ggtgagcgcc
      541 tctgccggaa cacgctgaaa cactcaagcg cctcatcgca acgacgctgg cgccgctgct
      601 cgatgtgggc ctgttttgcg ctttgatcgg ccaaggagcg tcgttgcgat ccagccatat
      661 cgccagcttc gctgtcgcca tggcgttgaa ttacttactg aaagttcgtt caacggtcgt
      721 tgcgcgtggg cgagggggcg acctgcgatt gcatggccga ttgatcatat tgagcttgat
      781 ggcgctgttt ctgcgcggcg gcgtcctcgg cttgctggcg caagattggg gctggcgacc
      841 gcaggttgcg attgtctttg cggtgctcgc cggactgacc gtgaccctcc ctggatacgc
      901 cgcagtactg tccgcggccc aaggtaacgc gcgggcgcgg gcgctcgcgg tggggctcat
      961 cgcctatggg ctggcgctgc ggctcgtgta ctcgggttcg gtggaactgc tccccgagga
     1021 agcctattac tggaactact cgcgccatct cgacatcggg tatctcgatc acccgccgat
     1081 ggtggcgtgg ctgatcaggc tcggaacggc gctgttcggt caatcggagt tcggcgtgcg
     1141 cgcgccggca ctcggctgcg cggcgattgc ctcggtcttc acctatcgcc tgacccgcaa
     1201 tctattcggc gagccgagcg cgcttgcggc gctgttgctg gcgcaggtgc tgccgtattt
     1261 ttttctctcg ggactgctca tgacgcccga cgcacccttg gtcgccgcat gggcggcctc
     1321 gctgtactat ctggagcgag cgctggtcgc cggactgatt ggctcctggt ggcgagcggg
     1381 acttgcgctt gggatcggcg gcctatcgaa atactccatc ggcctgctcg ttcccgtgac
     1441 gatcctgttc atgctgtggg atcctcgagc gcgcagcaac tggcgccgtt ggcaacccta
     1501 tgccgcagcg ctgctcgcgc tcgccgtgtt ctccccggtg attgtctgga atgcgcagca
     1561 tgattgggcg tccttcgtat tccaaacctc gcgacgtctc gccgaggcac ctcgattcgc
     1621 gctccacaag ctgctcgctt ccgccctcat tctcattact ccgaccggcg tgcttgccat
     1681 cgccgcggca ttcaccggcg cgaggcctga taacggcccc gccggggaca tgcggcggcg
     1741 ttggctgttc ctcggcatcg cgataggtct gccgctcgcg gtattcgcga tcttcagttt
     1801 gcgccatgaa gtaaagctcg actggaccgg cgcgccatgg ctcgcggcgc tgccggcgat
     1861 ggccgccgga atgagcggcg tcgaaacgag cacgcgcggc ttcgcggccc gtgtcggcgc
     1921 cgcgtggctg cccacggcgg tgacgctgct gctcatctac ggcgcgggcc tgcactacct
     1981 cgtgttggga cttcccggtc tcggctacgg cgctcacatc gaactcgttc cggtcgcctg
     2041 gcgcgacttg agccgccggg ttgccgccgc gacccaggca atccaccaac
//
LOCUS       DASWZT010000255         7440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_16341, whole genome shotgun sequence.
ACCESSION   DASWZT010000255 DASWZT010000000
VERSION     DASWZT010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7440
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16341"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VGE92_06945"
     CDS             complement(<1..390)
                     /locus_tag="VGE92_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY1313594.1"
                     /translation="MTRTSDTTARPAGAWLNGLLGVMIFSASLPATRIAVRDLNPLFL
                     SVARAAVAGILALALLWLGRQPRPARADLPGLARVALGVVFGFPLLSALALRDITSAH
                     ALLFIALLPLSTALFAVLLAHERPRPAF"
     gene            538..1812
                     /locus_tag="VGE92_06950"
     CDS             538..1812
                     /locus_tag="VGE92_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY1313595.1"
                     /translation="MSKSTVVEAYARLLAQGEISSRRGAGFFVAHSPPLSLAQAGRPV
                     EREIDPLWVMRRSLAADDLKLKPGCGWLPESWMPDAGIRRALRAAARGAAAPLVNYSE
                     PSGFQPLRECLSRLLGERGIGAAADQILLTDSGTQAIDLVCRLFIQPGDTVIVDDPCY
                     FNFLNIARAHRAKIVTIPYTPSGPDEGAFAQVVGEHSPRLYLTTGSFQNPTGATPSSI
                     AQHRILKLAEASNLIVVEDDIFADFAPPSTTRFAAFDGFERVIYVGSCSKTLSASLRC
                     GFICTRRDWIDSLVDLRLSTTFGGNEVATRAVHRLLLDGSYRKHMQTTQAKLAAAHAK
                     TSTRLMESGLRQWCQPRGGLFVWAELPDGLDAADVTRHALREAVMLAPGDVFSCNRSA
                     RGYLRFNVSQCNEARIFEILQRCIRACRKQRG"
     gene            complement(1813..2058)
                     /locus_tag="VGE92_06955"
     CDS             complement(1813..2058)
                     /locus_tag="VGE92_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313596.1"
                     /translation="MNRPLSLMCMILIIGTEARASESQRRINGCMIKRMTADRVMSYN
                     DAKRACLEELKALNARLNPGNLKPEAHASGGGETSGN"
     gene            2205..3470
                     /locus_tag="VGE92_06960"
     CDS             2205..3470
                     /locus_tag="VGE92_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY1313597.1"
                     /translation="MTQRKPVRPSEGLANVRYEIRGKLARRALALERSGYDIVSLNIG
                     NPYAFGFRTPETMRLAMIENLRNSEGYVHQKGIFPAREAVVMQQQERGVRGVTAEEVF
                     IGNGVSELIDLSLRALLNSGDEVLVPSPDYPLWTAAVNLNGGRAVHYPCHAAASFIPD
                     PEEIAALMTKRTRAIVIVNPNNPTGAVYPRETLAAIARFAEQHRLVVLSDEIYDQIVY
                     DAAEFVPMATLVHNTLCCTMSGLSKIYRACGYRVGWAAFSGDTESASEYLSGLELLSS
                     LRLCSNVPGQWAVQTALGGYQSIRELTRRGGRLYESRQVIIDAVARSKYLHLARPMGA
                     LYSFVEIRTDVLPDFDDQSFALDLLENKHVLVAPGVSFNVPYRNCFRITNLPEPQVLA
                     TVFGRIEELLDSQAAARPELQLVGSRNPM"
     gene            3467..6079
                     /locus_tag="VGE92_06965"
     CDS             3467..6079
                     /locus_tag="VGE92_06965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY1313598.1"
                     /translation="MTGLMAAVAAGHTVLAPNAELAAALFDAVERAHQACGREVWPTP
                     RVRDFGSWLLELHSKRQFEEAAAPRVLGDIDERELWRAVVESSDVGREILEPAGAARA
                     ARRARRAIREYGIPFSAVELDAASAEESRVFLEWNRRFEERCRALGCISAAELLGLTK
                     APAETIVWLESPVWRPMARQWLRRHGTMLAPERAVPQTACRLSSASPAAELATIADWA
                     LSQLAAPQFRAWICIPDLNLRRAEVIDAFDAVLAPQRFALNSAADAAVYAVAGGTPLA
                     EFAPVRAALDVLAASVDLVSFERFSALLRAPELHGSAGEAAAAARLDLQLRRRGPAED
                     DLAGWLALGERLAAAHEIGPVGALLKLRAAVHSLEAIRGEHPISRWVPAWIAALEAAP
                     WALRARWLSTEYQAAERFRELLGMLATADAIYGSLSRRAAQRLLRRAALETPFQVQTG
                     VPPIWVSGQLIDPWLGYDGLWVSGCSDARWPPPADPIPLLPIKLQREYGVIAAAPESQ
                     LQFALDLQRRWTERAAQCVFSYPDQGDGRCAAPSPLLPDTAAAPSAALPRPHWHALLQ
                     TAPALARIGDELAPAFATDERTRGVSTLRAQSRCAFRGFAETRLRAARLERPVPGFNE
                     RERGDLVHHALEHIWRTLRSSTGLSALSAEAQAALLDDAAGRALAIVCRERNPGTRWR
                     LRERERMHNVLQKWLEVERLRPPFEVATLEQDKQVVRHAGIEFSIRIDRVDQLTDGAR
                     VLIDYKTGRAARDWRGDRPDNPQLPLYALSRPEALVAVAYGRVNASECRFVAEAERDA
                     IFRPGRAKTTLEGEPSLAALLDVWSRRIENLAADFAAGRAAVAPTVRACRSCTLQGLC
                     RIPGALEDAVAADD"
     gene            6072..>7440
                     /locus_tag="VGE92_06970"
     CDS             6072..>7440
                     /locus_tag="VGE92_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY1313599.1"
                     /translation="MTDQAARERAIQAHGSVLVQAPAGSGKTTLLAQRYLRLLAQVDA
                     PERILALTFTRRAAQEMRDRVLAALSAARFSLCPEQMQPATWNLARAARRRLQELHID
                     VERQPSRLRIETIDAFNAWLASQLPVAAGSGSAFQVLADAQPSYVEAARRALAHEPGD
                     RFGMAVDRVLALDDQRWRKLLDLISEMLPNRDRWLPLLAGRLQSTRALDDTQLARVRR
                     HLDEDLELLITRVLNRALEVIGAERVDALSRLMQGAALHLGVARADLAECAADGSRLR
                     ADVGDLARWRAVASLLLIASGDLRTRFTKSEGFPAQSAAKAAILDLVQELGRDPRAKR
                     LLREIRVLPAAAYSDGEWTRVSDVAQVLVLAAAELQQVFREQGAVDFPEVSLAALRAL
                     GAADAPTDLGLRLDYRLQHILVDEFQDTSSAQLELVRLLTAGWQAGDGRSVFCVGDPM
                     QSIYGF"
BASE COUNT         1237 a         2331 c         2504 g         1368 t
ORIGIN      
        1 gaatgccggc cgcggccgct cgtgtgcgag cagtaccgcg aacagtgcag tcgataaggg
       61 cagcagcgcg atgaatagca gcgcgtgcgc ggaggtaata tcacgcagcg cgagtgcgct
      121 caacagcgga aagccgaaga ccacaccgag tgcgacccgc gcaaggcctg gaagatcggc
      181 gcgcgccggc cgcggctgac ggccgagcca caacagcgcg agcgcgagta ttcctgcgac
      241 cgcggcccga gcgacgctca ggaacagcgg gttgagatcg cgcaccgcga tgcgtgtagc
      301 cggcagcgag gcgctgaaga tcatgacccc caacaagccg ttgagccagg cgcccgccgg
      361 ccgcgccgtt gtgtccgaag tcctcgtcat gccggcaact gtggcacgcg ccaggtcgag
      421 attcgaggta cagaagggta caatttgggc aaactgtact gatgcattga gcaacacaga
      481 tggacgacat cagccgcgtt ggcttggtca tggcggacat tcgcaggcgc atccgcggtg
      541 tccaagtcca ccgtggtgga agcctatgcg cgtctgctgg ctcaaggcga gatcagctcg
      601 cgacgtggtg cgggtttctt tgtcgcgcac tccccgccgt tgtcgctcgc ccaagccggg
      661 cgtccggtcg agcgcgagat cgatcccttg tgggtgatgc gccgctcgct cgcggccgac
      721 gacctcaaat tgaagccggg ctgcggttgg ttgcccgaaa gctggatgcc ggatgcgggc
      781 attcgccggg cattgcgcgc ggcggcgcgc ggcgccgcgg caccgctcgt gaactacagc
      841 gagccttcgg gttttcagcc gctgcgagaa tgtctgtcgc gcctgctggg cgagcgcgga
      901 attggcgccg ccgcggatca gattctgctc accgattctg gaacccaggc gatcgacctg
      961 gtgtgccgtc tgttcatcca gccgggcgac acggtgatcg tcgatgatcc gtgctacttc
     1021 aatttcctga atatcgcgcg cgcacaccgc gccaagatcg tgacgattcc ctacacgccg
     1081 agcggtccgg atgaaggcgc gttcgcgcag gttgtcggcg aacactcacc gcggctttat
     1141 cttacgaccg gctcgttcca aaatcccacc ggagcaaccc cgtcctcgat cgcgcagcac
     1201 cgcatcctga agctggctga ggccagcaat ctcatcgtgg tcgaagacga catcttcgcc
     1261 gatttcgcac cgccgtcgac cacacgattt gcggcttttg acggtttcga gcgcgtgatc
     1321 tacgtcggca gctgttcgaa gacgctatcc gcctcgttgc gctgcggatt catctgcacg
     1381 cggcgcgact ggatcgacag tctggtggat ttgcggttgt cgacgacctt tggcggcaac
     1441 gaagttgcca cccgcgccgt gcatcggctg ctgctcgacg gtagctaccg caaacatatg
     1501 cagacgactc aagcaaaact tgcggcggcg cacgcgaaaa catcaacgcg gctgatggag
     1561 tccggactgc gacaatggtg tcaaccgcga ggcggattat tcgtatgggc cgagttgccg
     1621 gacggcctcg acgcggcgga tgtgacgcgg cacgcactgc gcgaggctgt catgcttgcc
     1681 ccgggcgatg tcttcagctg caaccgtagc gccagaggat acctgcggtt caatgtgtcg
     1741 caatgcaatg aagccagaat cttcgagata ttgcagcgct gcatccgcgc ctgccgcaaa
     1801 cagcgcggct gatcaattgc cgctcgtctc gccgccgccg gaggcatgcg cctcgggctt
     1861 cagattgccg ggattgagtc tcgcgttcag cgctttgagc tcctccaggc aagctcgctt
     1921 ggcgtcattg taggacatca cccggtcggc ggtcattcgc ttgatcatgc agccattgat
     1981 gcgccgctgc gattcggagg cgcgcgcctc cgtccctatg atcaggatca tgcacatcag
     2041 ggatagtggg cgattcaccg gcgggctcca agcaagtatt cgtttgcgag acgcagtatg
     2101 tcgcgccgcg ccgttgggac aagacgcaaa acacccctca agctgtcgga tttgctccga
     2161 catgcatttc gcctcgccgg cgatattccg ctattctgcg cgccatgact caacgaaagc
     2221 cggtgcgccc cagcgagggg ctggcgaacg tacgctacga aatccgcggt aagttggcgc
     2281 gacgcgcgct tgctttggag cgttcaggct acgacatcgt gtcgctcaat atcggcaatc
     2341 cctatgcatt cggcttcaga actccggaaa ccatgcgttt ggcgatgatc gagaacctgc
     2401 gcaacagcga aggctacgtg caccaaaagg gcatcttccc ggcgcgcgag gccgtggtaa
     2461 tgcagcagca ggagcgcggg gtgcgcggag tcaccgccga agaagtgttc atcggcaacg
     2521 gcgtcagcga attgatcgat cttagtctgc gcgcgctgct caattccggc gacgaggtgc
     2581 tggtgccgag ccccgactat ccgctgtgga ctgccgcggt gaacctcaac ggcgggcgcg
     2641 cggtgcacta cccctgccat gcagccgcct cgttcattcc ggatcccgag gaaatcgcag
     2701 cattgatgac caagcgtacg cgtgccatcg tcatcgtcaa ccccaataat cccaccggag
     2761 cggtctatcc gcgcgagacc ttggctgcga tcgcacgctt tgcggaacaa catcggctgg
     2821 tggtgttatc cgacgagatc tacgatcaga tcgtctacga cgcggctgag ttcgtgccca
     2881 tggcgaccct ggtccacaac acgctgtgct gtaccatgag cggtctgtcg aagatttatc
     2941 gtgcctgcgg ctatcgcgtc ggctgggccg cattctccgg tgataccgag agcgcgagcg
     3001 agtatctcag cggcctcgag ctcttgtcat cgctgcgcct gtgcagcaat gtgcccggtc
     3061 agtgggcggt gcaaacggca ttgggcggtt accagagcat ccgcgaactg acgcgccgcg
     3121 gcgggcgtct gtacgagtcg cggcaggtaa ttatcgatgc tgtcgcgcgc agcaagtatt
     3181 tgcatctggc ccgccccatg ggcgcgctgt attcattcgt ggagattcga accgacgtgc
     3241 tgccggactt cgacgatcaa agctttgcgc tcgacctgct cgagaacaag catgtgctgg
     3301 ttgcgccggg cgtgagcttc aacgtgccgt atcgcaattg ctttcggatc acgaatcttc
     3361 cagagccgca ggttctggca acagtcttcg ggcggatcga ggaattgctc gactcgcagg
     3421 ccgcggcccg gccggagctg cagttggtcg ggtcgcggaa tccgatgtga ccggcctgat
     3481 ggcggccgtc gcggccggcc acaccgtttt ggcgccgaac gcggagcttg cggctgcgct
     3541 gttcgatgcc gttgaacgcg ctcaccaagc ttgtggccgc gaggtttggc cgactccgcg
     3601 tgtccgggat ttcggcagct ggctgctcga gctgcattcc aaacggcaat tcgaagaggc
     3661 cgcggcaccg cgcgtactcg gcgatatcga cgagcgcgag ttgtggcgcg ctgtggtgga
     3721 atcgagcgat gtcggccggg aaattctcga gcccgccggc gctgctcggg cggcgcggcg
     3781 tgcgcggcgc gcgattcgcg aatacggcat ccccttttca gccgtggagc tcgatgcggc
     3841 gtcggccgag gagtcgcgcg tgtttctcga atggaaccgg cgcttcgaag agcgttgccg
     3901 cgcgctcggc tgtatcagcg ccgctgaact gctgggcttg accaaggccc cggccgaaac
     3961 catcgtatgg ctcgaaagtc ccgtgtggcg gccgatggcc cgtcaatggt tgcggcgtca
     4021 cggcacgatg ctggcgcccg agcgcgccgt gccgcaaacc gcgtgccggc tgagtagcgc
     4081 ttcgccggcc gcggaattgg caacgattgc cgattgggcg ctcagccaac tggccgcgcc
     4141 gcagtttcgc gcgtggatat gcattcctga cttgaatctg cgccgcgccg aggtcatcga
     4201 cgccttcgat gccgtattgg cgccgcagcg ctttgctttg aacagcgccg cggatgcggc
     4261 ggtctatgcg gtggcgggcg ggacgccgct cgcggagttt gcaccggtgc gagcggcact
     4321 cgatgtgctg gcggcgagcg ttgacttggt ttccttcgaa cgcttcagcg ctttgttgcg
     4381 cgcaccggaa ttgcacgggt ccgcgggcga ggcggctgcc gcggcacggt tggatctgca
     4441 actgcggcgt cgcggccccg ccgaggatga cctggcgggc tggctggcgc tcggcgagcg
     4501 gctggcggcg gcgcacgaaa tcggaccggt tggcgcattg ctgaagctgc gcgcggcggt
     4561 gcactcgctc gaggcgattc gcggcgagca cccgatcagc cgctgggtgc ccgcatggat
     4621 cgctgcgctt gaggcggcac cctgggcgct gcgcgcgcgc tggctcagta ccgaatatca
     4681 ggcggccgaa cgttttcgcg agcttctggg gatgctcgcg accgcggacg ccatttacgg
     4741 cagcctgtcg cggcgcgcgg cgcagcgtct gctacggcgt gcggcgctcg aaacgccatt
     4801 tcaagtgcaa accggcgtgc cgccgatttg ggtgagcggc caactcatcg acccctggct
     4861 cggttacgac ggcctgtggg tgagcggctg cagcgatgcg cgctggccgc cgcctgccga
     4921 ccccataccc ttgttgccga taaagctgca acgtgaatat ggtgtgatcg ccgcggcacc
     4981 ggaatcgcaa ctgcaattcg cgctcgattt gcagcgtcgt tggaccgagc gcgcggcgca
     5041 atgcgttttc agttatccgg atcagggcga cggccgttgc gccgcgccga gccccttgct
     5101 gccggacacc gcagcggctc cctcggcggc gttgccgcgg ccgcactggc atgcacttct
     5161 gcaaacggca ccggcgcttg cgcgcatcgg cgatgaactt gctcccgcct ttgcgacaga
     5221 tgagcgcacc cgcggtgtgt cgaccttgcg ggcgcagtcg cgctgtgcgt ttcgcgggtt
     5281 tgccgaaacg cgtctgcgcg ccgctcgcct cgagcggccg gtgccgggat tcaacgagcg
     5341 cgagcgcggc gacttggtgc atcacgccct cgagcacatt tggcgtaccc tgcgcagctc
     5401 cactggtctc agcgctctgt ccgcggaggc acaagcggcg ctgctcgatg atgcggcggg
     5461 acgggcgctc gcgatcgtat gccgggagcg taatcccggg acgcgctggc gacttcggga
     5521 acgcgagcgc atgcacaacg tcctgcaaaa atggcttgag gtcgagcggc tacggccgcc
     5581 gttcgaagtg gcgacgctgg agcaggacaa gcaggtcgtg cggcacgcgg gtatcgagtt
     5641 ctccattcga atcgatcgcg ttgatcaact gaccgacggc gcgcgcgtcc tcatcgacta
     5701 caagacggga cgggcggcga gagattggcg cggcgaccgg cccgacaatc cgcagttgcc
     5761 gctgtatgcg ctgtcgcggc ccgaggcgct ggttgcggtc gcctacgggc gcgtcaacgc
     5821 gagcgagtgt cggttcgtcg ccgaggccga acgagacgca atcttcaggc caggccgtgc
     5881 aaaaaccacc ctcgagggag agccgagcct cgccgcgttg ctcgatgttt ggtcgcggcg
     5941 cattgaaaat ctcgccgcgg attttgctgc gggccgcgct gccgttgcac ctaccgtgcg
     6001 cgcctgcagg agctgcacct tgcagggcct gtgccggata ccgggagcgc tcgaggacgc
     6061 ggtggcagcc gatgactgat caggcggcac gcgaacgcgc catccaggct cacggctcgg
     6121 tgctggtgca ggcgcccgcc ggctccggca agacaacatt gctggctcag cgctacttgc
     6181 gcctgctggc gcaagtcgat gcgcctgaac gcatcctggc gctgactttc acacggcgcg
     6241 ccgcgcagga aatgcgcgat cgggtgctgg ccgcattgag tgcggcgcgc ttctcgctgt
     6301 gtccggagca gatgcagccg gcgacctgga atctggcgcg cgctgcgcgg cggcgcttgc
     6361 aggaattgca catcgatgtc gagcgccagc cgtcgcggct gcgcatcgag acgatcgatg
     6421 cgttcaatgc ctggcttgca agccaactgc cggtagccgc aggttcagga tctgcctttc
     6481 aagtgctcgc cgatgcgcag ccgagctacg tggaggccgc gcgccgcgcg ttggcgcacg
     6541 agcccggcga tcgattcggc atggccgtcg atcgggtgct ggccctcgat gaccaacgct
     6601 ggcgcaagct tctcgatcta atcagcgaga tgcttccgaa ccgcgaccgc tggttgccgc
     6661 tgctcgccgg aagactgcag tcgacgcgcg cactcgacga cacgcagctc gcgcgcgtgc
     6721 gccggcactt ggatgaagat ctggaacttc tgataacacg ggttctgaac cgcgcactcg
     6781 aggtgatcgg cgccgaaagg gtcgacgcct tgtcgcgtct gatgcagggg gccgcgctac
     6841 atctcggagt agcgcgcgct gatcttgccg aatgcgcagc agatggctcc cgattgcgtg
     6901 ccgatgtcgg cgatctggca cgctggcgcg cagtcgcgtc gctgctgctg atcgcgagcg
     6961 gcgacttgcg cacgagattc accaagagcg aaggcttccc ggcgcaatcc gcagccaagg
     7021 cagcgattct cgatctggtg caggaattgg gccgcgatcc gcgcgcaaaa agactgttgc
     7081 gggagattcg cgtgctgccg gcggccgctt acagcgacgg cgagtggacg cgcgtcagcg
     7141 acgtggctca ggtgctggtt cttgccgccg cggaattgca gcaagtgttt cgcgaacagg
     7201 gcgcggtcga ctttccggag gtgtcgttgg cggcgctgcg tgcgctcggt gcggcggatg
     7261 cgccgaccga tcttggactg cgactcgact atcggctgca gcatatttta gtcgacgaat
     7321 tccaggacac ctcgagcgcg cagctggaat tggtgcgatt gttgaccgcc gggtggcagg
     7381 ccggcgacgg ccgcagcgtg ttttgcgtcg gcgatccgat gcaatcgatt tacggctttc
//
LOCUS       DASWZT010000256         1566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_26142, whole genome shotgun sequence.
ACCESSION   DASWZT010000256 DASWZT010000000
VERSION     DASWZT010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26142"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1566)
                     /locus_tag="VGE92_06975"
     CDS             complement(<1..>1566)
                     /locus_tag="VGE92_06975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY1313600.1"
                     /translation="RYTADPEVLVKTSGGASIAVLVVRPKSAAKPQPTLLEFTIYDSQ
                     NYAKECAAHGYAGVVAFTRGKHTDPDKVIPYQHDGEDARAVIEWIAKQPWSDGRVGMY
                     GNGYSGFASWAAAKHPPAALKAIATTSASTPGVDVPMGGGIFQNSAYRWSSYVTNTKE
                     ADAKGYYEDALWRALDQKWYRSGKRYRDLGRLHGKPNPIFIRWLNHPSYDRFWQKMVP
                     YREQFAHINIPVLTTTGYYAGGEPGALYYFTQHYRYNPHADHTLLIGPYDDTVMQRGP
                     SATLQSYEVDPAALLDLRELRYQWFDHLFKGAAAPALLKDRVNYEVMGANEWRHAGSL
                     EAMATGTLKFYLEGGGSGERHHLSQHKSAHPTALRQTVSLTDRRDFAWTEPADFPSRN
                     LATHNAVMFVSEPLAKSTEFDGLFAGKLDFTVNKMDVDLNVAMYELLPSGDYIYLFNP
                     TYEFRASYAKDRVNRHLLKAGERQQLLFKSERLTSRRFQPGSRLVMILGINKRPDREI
                     NYGTGDDVSEDSIA"
BASE COUNT          270 a          475 c          489 g          332 t
ORIGIN      
        1 gcgatggagt cctcgctcac gtcatcgccg gtaccgtaat tgatctcgcg atccggccgc
       61 ttgttgatgc cgagaatcat caccagccgg ctgcccggct ggaatcgccg gctcgtcagc
      121 cgttcgctct tgaacagcaa ctgctggcgc tcgcccgctt tgagtagatg gcggttgacg
      181 cgatctttgg cgtagctcgc gcggaattcg taggtgggat tgaacaggta aatatagtcg
      241 ccgctcggca acagttcata catggcgaca ttcaaatcga cgtccatctt gttcaccgtg
      301 aaatcgagct tgcccgcaaa caggccgtcg aactcggtgc tctttgccaa gggctcgctc
      361 acgaacatga ccgcattgtg tgtcgccaaa ttgcggctgg gaaagtccgc cggttcggtc
      421 caggcaaagt cgcggcggtc ggtcagactc accgtctgcc gcaaggcagt cggatgcgcg
      481 ctcttgtgtt ggctgaggtg gtgccgctcg ccggagccgc cgccctccag ataaaatttc
      541 agagtgcccg tggccatggc ttcgagcgag ccggcatgcc gccattcgtt cgctcccatg
      601 acctcgtagt tgacgcgatc cttcaacagg gcgggtgccg cagcgccttt gaacaagtgg
      661 tcgaaccact gatagcgcaa ttcacgcagg tcgaggagtg cggccggatc cacttcgtag
      721 ctctgcagcg tggccgacgg cccgcgttgc atcaccgtgt cgtcgtaagg cccgatcagc
      781 aaggtgtggt ccgcgtgcgg attgtatcga tagtgctgcg tgaaataata gagcgcgccc
      841 ggctcgccgc cggcgtagta gccggtagtc gtgagcaccg gaatattgat gtgcgcgaac
      901 tgttcgcggt acggcaccat cttctgccag aaccgatcgt agctcgggtg attcagccag
      961 cggatgaaga tgggattcgg cttgccgtgc agccggccga gatcgcggta gcgcttgccg
     1021 ctgcggtacc acttctgatc gagggcacgc catagcgcgt cttcgtagta gcctttggcg
     1081 tcggcttcct tggtgttggt cacgtaggaa gaccagcgat acgcggaatt ctggaagatg
     1141 ccgccgccca tgggtacatc gaccccgggc gtgctcgccg acgtggtcgc gatggctttg
     1201 agcgccgccg gcgggtgttt cgcggccgcc caagacgcga agccgctata gccgttgccg
     1261 tacatcccga cgcggccgtc gctccacggc tgcttcgcaa tccattcgat gaccgcgcgt
     1321 gcgtcttcac cgtcatgctg atagggaatt accttgtccg ggtcggtatg cttgcctcgc
     1381 gtgaaagcga ctacacccgc gtagccgtgc gcagcgcact ccttggcgta gttctgcgag
     1441 tcgtagatcg taaactcgag cagtgtcggc tgcggttttg cggcgctctt cggcctaacc
     1501 accagcaccg caatgcttgc gcccccggaa gtctttacca gaacctcggg gtcggccgtg
     1561 tagcgc
//
LOCUS       DASWZT010000257         2862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_45536, whole genome shotgun sequence.
ACCESSION   DASWZT010000257 DASWZT010000000
VERSION     DASWZT010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45536"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..432)
                     /locus_tag="VGE92_06980"
     CDS             complement(79..432)
                     /locus_tag="VGE92_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY1313601.1"
                     /translation="MQPAGVRLCRLEEIPDGDSRGFDPLNTGQDTMLVVRQGGALFAW
                     RDACPHINGVPMAWRKNAYLNAGRDRIVCSAHGALFDIATGLCTLGPCLGQSLEPMNV
                     LVADDGSLYWQPRSN"
     gene            complement(459..1403)
                     /locus_tag="VGE92_06985"
     CDS             complement(459..1403)
                     /locus_tag="VGE92_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY1313602.1"
                     /translation="MQIIGPDSLIFGVDDVAACIQYLTDYGLTPRDVSAAGGRFEALD
                     GTAIVIRHKNDASLPAPMGTASMLRKTVYGVADAATLTAVAAELGKDREVRRLADGSL
                     EALDNMGFVLGFQITVRRKFSLPAEAINAPGAPAQRPANVVAVDEAAVIVPRTLSHVV
                     YFVPDAAKAEAFYQRLGFRCTDRFIGAGPFLQPAGSLDHHTLFLIQTPPAMLGCEHFT
                     FHLAGPTELMQAGYRFVNKGYQSFWGPGRHKFGSNWFWYFNSPLGCHVEYDADMDLHD
                     ASWVAREVPINADSSQLFLFHHREKWSPGGPPAPGAAH"
     gene            complement(1413..2576)
                     /locus_tag="VGE92_06990"
     CDS             complement(1413..2576)
                     /locus_tag="VGE92_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY1313603.1"
                     /translation="MFRYFPTNYVWNLSVDLAIEMGARIGEIEEMCAPLMEASKSPDA
                     EGTRAFARSWVSMGDKLASLAAEDEEHGRLISAGDKLQRAAIYYLTAERMQGHGSPGR
                     AELYAQMQRAFARGVTLSGANCERLQIPYANVHLSGLYVRAEGVTGRAPLLVQVNGLD
                     STKEMKYFVGLPRWLAQRGVASLLIDQPGSGEALRLHGMKAVYNSELWASKVLDYLET
                     RTDVDPQRIGLEGVSLGGYYCPRAVAFEPRFAMGVCWGANHDWRDVQKKRLLREGSFP
                     VPHYWDHVRWVWGGDSIDEFMKIAEQVHLDGVLDRIKVPFLVTHGEKDSQIPLHWAER
                     TYDQLVNSPKRELKIFTAREGGVQHSSFDNSANAGAFIADWVAETLGGRIAHL"
BASE COUNT          555 a          918 c          861 g          528 t
ORIGIN      
        1 ggccaagacc ttgggccgcg ccggtcacca gggcgacccg accttgcaga tcgaacaatc
       61 ctttggcatc aagcccggtc aattcgatct cggctgccag taaagcgagc cgtcgtcggc
      121 gaccaggaca ttcatgggtt caagcgattg acccaggcag gggcccagcg tgcataagcc
      181 ggtcgcaatg tcgaataggg cgccgtgcgc actgcagacg atgcgatcgc gcccggcgtt
      241 aagatacgca ttcttgcgcc aggccatcgg taccccgttg atgtgtgggc aggcatcgcg
      301 ccaggcgaac agcgcgccgc cttggcgaac caccagcatc gtgtcctgcc ccgtattgag
      361 cggatcgaag ccgcgcgaat caccgtccgg aatctcctcc aggcggcaca ggcgtacacc
      421 ggccggctgc atctagggcg cgccgcccaa acgcgctgtc aatgcgccgc cccaggcgcc
      481 ggagggccgc caggagacca cttctcgcga tgatgaaaca agaacaattg cgaagagtcg
      541 gcattgatcg gtacttcgcg cgccacccaa gaggcgtcgt gcaaatccat atcggcatcg
      601 tattcgacgt gacaacccag cgggctgttg aagtaccaga accagttcga gccgaacttg
      661 tgccgtccag gaccccagaa cgactgatag cccttgttca cgaagcgata ccccgcctgc
      721 atcaattccg tgggtcccgc caagtgaaac gtaaaatgct cgcagcccag catcgcgggc
      781 ggcgtctgga tgagaaacag cgtgtggtga tcgagcgatc ccgccggctg caagaacgga
      841 ccggcgccga tgaagcggtc ggtacagcgg aaacccaacc gctggtagaa cgcttcagcc
      901 ttggccgcgt ccggcacgaa gtacaccacg tgcgagagcg tgcgcggtac gatcaccgcc
      961 gcctcgtcca cggcgacgac attggccggc cgctgcgccg gagcacccgg cgcgttgatc
     1021 gcttcggcag gcaggctgaa tttgcgccgc acggtgattt gaaagccgag cacgaaaccc
     1081 atgttgtcga gcgcttcgag cgacccgtcg gcgagcctgc gcacctctcg atccttgccc
     1141 aactcggctg cgacggcggt gagagtcgcg gcgtcggcga cgccgtatac ggtcttgcgc
     1201 agcatgctcg ccgtgcccat cggggccggc aggctggcat cgttcttgtg gcgaatcacg
     1261 atcgcggtgc cgtcgagcgc ctcgaatcgg ccgccggcgg cgctgacatc gcggggcgtc
     1321 agaccgtaat cggtgaggta ttgaatgcag gcagcgacgt cgtcgacgcc gaaaatgagt
     1381 gagtctggtc caatgatttg catgttgatt ctctagaggt gagcgatgcg gccgccgagc
     1441 gtctcggcaa cccaatcggc gatgaaggca ccggcatttg ccgaattgtc gaagctcgag
     1501 tgttgtacgc cgccctcgcg cgcggtgaat attttcagct cgcgcttcgg gctgtttacc
     1561 aattgatcgt aggtgcgctc ggcccaatgc agcgggattt gcgaatcctt ctcgccatgc
     1621 gtcaccagga acggaacctt gatgcgatcc aatactccgt ccagatgtac ctgctctgcg
     1681 attttcatga attcatcgat gctgtcgccg ccccataccc aacgcacgtg atcccaataa
     1741 tgcggcaccg gaaagctgcc ttcccgcagc agccgctttt tctgcacgtc gcgccaatcg
     1801 tgattcgcac cccagcatac gcccatggcg aaacgcggct cgaaggcgac ggcccgcgga
     1861 cagtagtagc cgcccaatga cacaccctcg agaccaattc tctgcggatc gacgtcggtg
     1921 cgagtctcga gataatcgag tactttgctc gcccataatt cgctgttata gaccgccttc
     1981 atgccgtgca agcgcagcgc ttcgccgctg cccggttggt ctatcagcaa cgaggcgacg
     2041 ccgcgctgcg cgagccagcg cggcagacca acgaagtact tcatctcttt ggtgctatcc
     2101 aagccattga cctgcaccag gaggggtgca cggccggtga ccccctcggc gcgtacgtaa
     2161 agaccgctga ggtgcacgtt cgcataggga atctgcagcc gttcgcaatt ggcgcccgac
     2221 aacgtgacgc cgcgcgcgaa cgcgcgctgc atctgcgcat acaattcggc tcgcccaggg
     2281 ctgccgtgtc cctgcatgcg ttcggcggtc aaataataga tggccgcgcg ctgtaatttg
     2341 tcgccggcgg agatcaggcg cccatgctcc tcgtcctcgg ccgcgaggct cgcgagcttg
     2401 tcgcccatgc tgacccagct gcgcgcaaac gcgcgagtgc cctcggcgtc cggcgacttg
     2461 gaggcctcca tcagcggcgc gcacatctcc tcgatctcgc cgatccgcgc gcccatttcg
     2521 atcgccagat caaccgacag gttccagacg tagttggttg gaaaataacg gaacatgtct
     2581 gccgcctcaa ggatgcagcg cgcgctgcag tgaggcagtt taccgttacc agccccgctc
     2641 gatacgcgat tggcgtgatg gttgatatcg ccctcgtcga tacattaccc tgcaacgcgt
     2701 atgcgcttcc aacaactgga cctgaatctg ctggtggcat tggacgcctt gttgagcgag
     2761 cgcagcatca cactcgcggg ccagaagctg catttgagtc agtccggcat gagttccggg
     2821 ctcgcgcgat tgcgcgaata cttcggcgac gaattgttgg tc
//
LOCUS       DASWZT010000258         1554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_31128, whole genome shotgun sequence.
ACCESSION   DASWZT010000258 DASWZT010000000
VERSION     DASWZT010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31128"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VGE92_06995"
     CDS             complement(<1..291)
                     /locus_tag="VGE92_06995"
                     /inference="COORDINATES: protein motif:HMM:NF018866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 family protein"
                     /protein_id="HEY1313604.1"
                     /translation="MSNEEIIAATRRWLERSVIGLNLCPFAESVYRGGRVRLRVSEQR
                     SAAALLEELRLELAALQAADPRDCETTLLIHPWVLNDFVEYNDFLEVCDATLA"
     gene            373..1404
                     /locus_tag="VGE92_07000"
     CDS             373..1404
                     /locus_tag="VGE92_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEY1313605.1"
                     /translation="MPQSIPATLIPGDGIGPEIVESTVAVLTALGAPFEWDIQAAGLE
                     GIKSAGDPLPAKTIDSIRRTRLALKGPLETPSGGGYRSSNVRLREEFKLYANLRPART
                     LVPGGRFDKIDLVVVRENLEGLYIGHEHYIQIDDDPHAVAMATGVNTRQGSRRLLEFA
                     FEHAIATRRKKVTIVHKANIMKALTGIFLETGLQLYERKYKDRFVLDTVIIDACAMKL
                     VLNPWQFDVLVTTNLFGDILSDLVAGLVGGLGMTPGANIGANAAIFEAVHGSAPDIAG
                     KGVANPTALLLAAAMMLDHCHLPHMAARLRTAIDQALNLDNAKTADLGGHTSTADFTR
                     ALVKRIARN"
     gene            1415..>1554
                     /locus_tag="VGE92_07005"
     CDS             1415..>1554
                     /locus_tag="VGE92_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1313606.1"
                     /translation="MAAVISIRDVVKTYATGFQALKLVNLEIERGEIFALLGPNGAGK
                     TTL"
BASE COUNT          313 a          507 c          451 g          283 t
ORIGIN      
        1 ggccagcgtc gcgtcgcaca cctcgagaaa gtcgttgtac tcgacgaagt cgtttaacac
       61 ccacggatgg atcaacaggg tggtctcgca atcccggggg tcggccgcct gcagagcagc
      121 cagttccaaa cgcaattcct cgagcaatgc cgctgcggaa cgctgttcgc tgacgcgcaa
      181 acgcacgcgc ccgccgcggt acacgctttc ggcgaacgga cacaggttaa ggccgatgac
      241 cgagcgttcg agccagcggc gcgtcgccgc aattatttct tcgttgctaa taagtaattc
      301 ccgccgcgaa gtgtaatcgc ctccattcta gtatcctaat cacttgcccc ggtcacttgc
      361 gagccagcga cgatgccaca gtccattcca gccaccctga tccccggcga tgggatcggt
      421 cccgaaatcg tcgaatccac agtcgcggta ttgacggcgc tcggcgcacc gttcgagtgg
      481 gatatccagg ccgcgggtct cgaaggcatc aagagcgccg gcgatcccct gccggcgaag
      541 accatcgaca gcatccgccg cacgcgcctt gccttgaagg gcccgctcga aactccgtcg
      601 ggcggcggct atcgttcctc gaacgtgcgc ctgcgcgaag aattcaagct gtacgcaaac
      661 ctgcgccccg cgcgcacgtt ggttccgggc ggccgcttcg acaagatcga tctggtcgtc
      721 gtgcgcgaga acttggaagg tctgtatatc ggccacgagc actatatcca gatcgacgac
      781 gacccacacg ccgttgccat ggcaaccggt gtgaacacgc ggcagggaag ccgccggctg
      841 ctcgagttcg ctttcgaaca cgccatcgcc acccgccgca agaaagtgac gatcgtgcac
      901 aaggccaaca tcatgaaggc gcttaccggg atttttctgg aaaccgggct gcagctgtac
      961 gaacgcaagt acaaggaccg cttcgtcttg gacaccgtga tcatcgacgc gtgcgccatg
     1021 aagctcgtgc tgaacccttg gcaattcgac gtgctggtca cgaccaacct gttcggcgat
     1081 atcctctccg acctggtagc cgggctggtc gggggattgg gcatgactcc gggtgccaat
     1141 atcggcgcga atgcggcgat ttttgaagcc gtgcacggct ccgcgcccga tattgccggc
     1201 aagggagttg cgaaccccac ggcgctgctg ctcgcggccg ccatgatgct cgatcactgc
     1261 cacttaccgc acatggcggc gcgcctgcgc accgccatcg atcaggcgtt gaacctcgac
     1321 aatgcgaaaa ccgctgacct cggcggccat accagcaccg ccgatttcac gcgcgcgctg
     1381 gtgaagcgca ttgccagaaa ctgactgagc gttcatggct gccgtcattt ccatccgcga
     1441 cgtggtcaag acctatgcga ccggctttca agcgctcaag ctcgtgaatc tcgagatcga
     1501 aagaggggaa atcttcgcct tgctcgggcc caatggcgcc ggcaagacca ccct
//
LOCUS       DASWZT010000259         2013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_6675, whole genome shotgun sequence.
ACCESSION   DASWZT010000259 DASWZT010000000
VERSION     DASWZT010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6675"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1053)
                     /gene="ppk2"
                     /locus_tag="VGE92_07010"
     CDS             complement(115..1053)
                     /gene="ppk2"
                     /locus_tag="VGE92_07010"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEY1313607.1"
                     /translation="MKDKAGKGNPALDHEELVRRISRDLADSYDEELEMEIEDRHPLP
                     GLPTSDDGDAEKAYRRRYFTELFRLQAELVKLQDWTVAAQQKVVILFEGRDAAGKGSA
                     IKRITQRLNPRVCRVVALPAPNDRERTQWYFQRYIPHLPAGGEIVLFDRSWYNRAGVE
                     KVMGFCTAEEYEEFFRSVPEFERMLVRSGIRLLKYWFSITDEEQYLRFLGRIHDPLKQ
                     WKLSPMDLESRRRWEEYTKAKEVMLERSHIPEARWWVVHAVDKKRARLNCIDHLLKQF
                     PYNEVQKPPVVLPERERHEHYSRQAVPAAMLVPEVY"
     gene            complement(1065..>2013)
                     /locus_tag="VGE92_07015"
     CDS             complement(1065..>2013)
                     /locus_tag="VGE92_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEY1313608.1"
                     /translation="GLARLKPLADDGHFLDLFAQVKLESKRQCAAWLNSKFGVAVDPS
                     SIFDCHIKRIHEYKRQLLNALRIIVLYNRLRENPRLDMAPRTFFFAGKAAPAYTLAKL
                     IIKFIDSLARTIAALPAANRKLQVVFLPDYDVSLAQRLIPASDVSNQISTAGYEASGT
                     SNMKFMMNGALTLGTRDGATIEMAREAGEENFFLFGLSADQVAANRGRYDPRWHYAHD
                     AETRAALDLIAGGHFGGDAGLFAPLYNTLTQTDYYMHLADLQSYLEADQRLRDLYARP
                     REWARKALLNVAASGKFSSDRTIAEYAVGIWGVSACPVP"
BASE COUNT          376 a          606 c          612 g          419 t
ORIGIN      
        1 gccgagagct taccccgcca cgctcgcggg cagcaaaggc ggatcgtatg acgcaggagg
       61 tatattgtgt aagcctatat ttttattcga cttctgcttc tgcccgtcag acgatcaata
      121 cacctcgggc accagcatcg cggccggcac cgcctggcgt gaatagtgtt catgccgttc
      181 gcgctccggc aggacgaccg gcggtttctg cacttcgttg tatggaaact gcttcagcaa
      241 atgatcgatg caattgagcc gcgcccgctt cttgtcgacc gcatgcacca cccaccagcg
      301 cgcctcggga atgtgcgagc gctcgagcat cacttccttg gccttggtgt attcctccca
      361 gcggcggcgc gactccagat ccatcgggct caatttccac tgtttgagcg ggtcgtggat
      421 tcggccgagg aaccgcaggt attgctcctc gtcggtgatc gagaaccaat atttgagcaa
      481 gcggattccg gaacgtacca gcatgcgttc gaattcgggc acggatcgaa agaactcttc
      541 gtactcctcg gcggtgcaaa agcccatcac cttctcgact ccggcgcggt tgtaccaact
      601 gcgatcgaac agcacgatct ccccgccggc cggaagatgc gggatatagc gctggaagta
      661 ccactgcgtg cgctcgcggt cgttgggagc cggcagcgcc accacccggc agacgcgcgg
      721 attcagacgt tgggtgatgc gcttgattgc gctgcctttg ccagcggcgt cgcgcccttc
      781 gaaaagaatg acgactttct gttgggcggc aacggtccaa tcctgcagct tgaccaattc
      841 agcttgcagg cgaaacagct cggtgaagta gcgccgtcga taggcttttt cggcatcgcc
      901 atcatcgctc gtgggcagcc cgggaagcgg gtgccgatcc tcgatctcca tctcgagttc
      961 ttcgtcgtag ctgtccgcga gatcgcggct gatgcgtcgc accagctctt cgtggtcgag
     1021 cgccgggtta cctttacccg ctttgtcttt catcggcttc tccttcaagg gaccgggcat
     1081 gcggagactc cccagatgcc gacggcgtat tccgcgatcg tacgatcgct cgagaatttt
     1141 ccggaagcgg ccacgttgag cagcgctttg cgggcccatt cgcgcggcct ggcatataaa
     1201 tcgcgcaacc gctgatcggc ttccagatac gactgcaaat ctgccaggtg catatagtag
     1261 tccgtctggg tcagcgtgtt gtagagcggc gcgaacaaac cggcatcgcc gccgaagtgt
     1321 ccgccggcga tcaggtcgag agcggcgcga gtttccgcat cgtgcgcata atgccagcgc
     1381 gggtcgtaac ggccgcggtt cgccgcgact tggtcggcgc tgaggccaaa cagaaagaaa
     1441 ttctcctcac cggcctcgcg cgccatttcg atggtggcgc cgtcgcgcgt acccagggtg
     1501 agcgcgccgt tcatcatgaa tttcatgttg ctggtaccgc tcgcctcgta ccccgcggtg
     1561 gaaatctgat tcgacacgtc gctcgcgggg atcagccgct gtgccagcga gacatcgtag
     1621 tccggcaaaa agaccacctg cagtttgcgg ttcgcggccg gcagggccgc aatggtccgc
     1681 gccaggctgt cgatgaactt gatgatcagt ttcgcgagtg tgtaagcggg agcggccttg
     1741 ccggcaaaga aaaacgtccg cggtgccatg tcgagccgcg gattctcgcg cagccggttg
     1801 tacagcacga tgatgcgcag cgcgttgagt agctgacgct tgtactcgtg gatgcgtttg
     1861 atgtgacaat cgaaaatcga gctcggatcg accgcgacgc cgaacttcga attgagccac
     1921 gcagcgcatt gccgcttgct ctcgagcttc acctgggcga atagatcgag aaaatgtccg
     1981 tcgtcggcca ggggcttgag acgggcaagg ccg
//
LOCUS       DASWZT010000260         4453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_88934, whole genome shotgun sequence.
ACCESSION   DASWZT010000260 DASWZT010000000
VERSION     DASWZT010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88934"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1218)
                     /locus_tag="VGE92_07020"
     CDS             complement(<1..1218)
                     /locus_tag="VGE92_07020"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1313609.1"
                     /translation="MWIVRVALQRPYTFIMLAVLIVLMGGYAILNTATDIFPNIKIPI
                     AAVIWRYNGILPEEIANRIVLFSERVAQTTVNDVEHTESQSVNGTAVVKYFFQPDADN
                     DLSMSQITAVSQVQLAYSPPGTTPPFVLNYSASSVPILQLALSSDTLPEAQIYDLANT
                     LLRTALATVPGASIPFPFGGKQRQVQVDLDPQALRARALSANDVVAAIGAQNLILPAG
                     TQKIGPLEYYIKLNASPTQIEDLNNLPIRAHNGNITYIRDVAHVRDGYSPQTNVVRLE
                     GRRSVLMSVLKTGKASTIDIINSINQKLPQIRAALPAELKIEPLSDQSVFVRAAISGV
                     VREAAIAGALTGLLILLFLGSWRSTLIITISIPLSILASISCLAALGETINIMTLGGL
                     ALAVGILVDDATVT"
     gene            complement(1300..1626)
                     /locus_tag="VGE92_07025"
     CDS             complement(1300..1626)
                     /locus_tag="VGE92_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313610.1"
                     /translation="MQRGSVRGPVTMCELATLMVMDRSTLGHNLKPLERDDLIVLRLS
                     PADRRKRYVELTKKGRSTLHQSRRLWRRAEHRFERIFGKQSAAELRAVLLGIAGNEEL
                     NALAIR"
     gene            complement(1752..2684)
                     /locus_tag="VGE92_07030"
     CDS             complement(1752..2684)
                     /locus_tag="VGE92_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY1313611.1"
                     /translation="MLKSTPFGMRSTADQVLAGIDLTGKRFVVTGCTSGLGFETMSAL
                     AANGAQVIGLARSLERAEIACRKAGPLCIPVACDLADLDSVAAAADTIRSLHDQLDAI
                     VANAGIANLPFLETRYGVEMQFLVNHIGHFALLNGILDLVKVGTGRIVIVSSSASVDY
                     APAEGIMFNNLDGGRFYKPFTFYGQSKLANALFAKELSRRLDARGIAVNSLHPGATRS
                     TGLHRNLSFMPRLALTAAQWFMRSAQRGAATQALLAAHPAVAGISGEYWSNCQIAAGS
                     RLLQDAELAARLWSVSEQIIARHAALPAALRQAA"
     gene            complement(2677..3300)
                     /locus_tag="VGE92_07035"
     CDS             complement(2677..3300)
                     /locus_tag="VGE92_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY1313612.1"
                     /translation="MEIIHNPEIEQTDVRLLPALDAAVAVFARFGFRKTSMDEVARAA
                     GVSRQGLYLQFANKEELFRKALVHTLSQQLNAASAALERADESLELRLIAACDAWSGR
                     FVGSLGADAADLMCASTSLAGTTLTHYETQFEAAVAGAIANSALGEVCGLIGVRAADL
                     ARALHATARGLKHSCASREEFLRGMTAAARMVCVPLNPRLQRGIADA"
     gene            3475..>4453
                     /locus_tag="VGE92_07040"
     CDS             3475..>4453
                     /locus_tag="VGE92_07040"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEY1313613.1"
                     /translation="MDLKSDTHGLPFPPGASALMTDIPRLRAGRVAGQFWSVWIPSGM
                     QGFEAVQTTLEQMDLVKLMAARYPDDLEMAYSAADVRRIHRSHKVASMIGIEGGHQIN
                     NSLAVLRQMYDAGARYMTLTHTLNTGWADSATDAPLHHGLTPFGVEVVREMNRLGMLV
                     DLSHVSAETMRAALSASAAPVIFSHSSARALVDHPRNVADDILRSVAANGGVVMVNFA
                     PGYVSESRNRWDADQAAERARFNSPPYVGLYIGQPERAKAAVAVWEENHPRPEVTLAQ
                     VADHIDHIRQVAGADHVGLGSDFDGIEDAPVGLEGVDRYPLLLAELMRRG"
BASE COUNT          865 a         1341 c         1407 g          840 t
ORIGIN      
        1 ggtcacggtg gcatcgtcca ccaggatgcc tacggcgagt gccagcccgc cgagcgtcat
       61 gatgttgatc gtctcgccga gcgccgccaa gcaggaaata gaggcgagga tcgacaacgg
      121 aatggaaatg gtgatgatca atgtgctgcg ccagctgccg aggaacaaca ggatcaacaa
      181 tccggtcagg gcaccggcga tcgccgcctc gcgaaccact cccgagatgg cggcgcgcac
      241 gaacaccgat tgatcgctca gcggctcgat tttcagctcc gcgggcagcg ctgcacggat
      301 ctgcggcagc ttctggttga tgctgttgat gatgtcgatg gtggaggcct tgccggtctt
      361 caatacgctc atcaagaccg agcgtcgccc ttccaagcgc accacgttgg tctgcggcga
      421 gtagccgtcg cgcacatgcg ccacatcccg aatgtaggta atgttgccgt tgtgcgcgcg
      481 tatcggcaga ttgttcagat cctcgatctg ggtcggactg gcgttgagct tgatgtagta
      541 ttcgaggggt ccgatcttct gcgtacctgc gggcagtatc aggttttgcg cgccgatggc
      601 ggcgaccacg tcgttcgccg acagcgcacg cgcgcgcagc gcctgcggat cgagatccac
      661 ctgcacttgc cgctgcttgc cgccgaacgg aaatggtatc gaggcgccgg gcacggttgc
      721 cagtgcggtg cgcagcagcg tattggcaag atcgtagatt tgcgcttcgg gcaaggtatc
      781 gctcgacaag gcgagctgca ggatgggcac gctcgacgcg ctgtaattca gaacgaaggg
      841 cggcgtcgtc cccggcggcg agtatgcaag ctgaacctgc gacacggcgg tgatctgcga
      901 catcgacaaa tcgttgtcgg cgtccggttg aaagaagtac ttgacgaccg cggtgccgtt
      961 caccgattgg gattcggtgt gctccacatc gttcaccgtg gtttgcgcga cgcgctcgga
     1021 aaacaatacg atgcgattgg cgatttcttc gggcaggatg ccgttgtagc gccaaattac
     1081 cgcggcaatc ggaatcttga tgttcggaaa aatgtcagtc gcggtgttga ggatcgcata
     1141 gccgcccatt aaaacgatca acaccgccaa catgatgaag gtgtagggcc gctgcaacgc
     1201 tactctgacg atccacatgc tacgctccga ttggcttgcg attcagtggc gttcggtgcc
     1261 ggtcaaaccg cgcgcagcgc cgccgctgtg gctggtcagc tagcgaatcg ccagtgcgtt
     1321 caactcctcg ttgccagcga tgccgagcag aaccgcccgc agctccgctg cggattgttt
     1381 gccgaatatc ctctcgaatc gatgttcggc gcgtcgccag aggcgtcgcg attggtgcaa
     1441 agtcgagcgg ccttttttcg tcaactcgac gtagcgttta cgacgatccg ccggcgacag
     1501 gcgcaggacg atcaaatcat cgcgttccaa gggtttgagg ttatgcccta acgtcgatcg
     1561 atccatcacc atcagggtcg ccagctcgca catcgtgacc gggccgcgta cgctgccgcg
     1621 ttgcacctcg gagaggatgg aatacgctgt gctgcatcgg ctgcgaattg cgcgtcggcg
     1681 aactgcggcg gcttgcgggc atatgcccat atcataagat gaaaccggca acaaatcatc
     1741 gccggcccga tttacgccgc ctgtcgcagc gcggcgggga gtgccgcgtg tcgcgcgatg
     1801 atctgctccg atacgctcca gagccgggcg gcgagctcgg cgtcctgcag caggcggctg
     1861 ccggcggcga tctgacaatt cgaccaatat tcgccgctga ttccggcgac ggcgggatgc
     1921 gccgccagca acgcttgggt ggcggcgccg cgctgcgccg agcgcatgaa ccattgcgcc
     1981 gccgtcaagg cgagtctcgg catgaaactc aaatttctgt gtagaccggt ggagcgcgtc
     2041 gcgcccggat gcagcgaatt gacggcaatg ccgcgcgcat caagccggcg cgacaactct
     2101 ttggcgaaca gcgcgttggc caatttggac tggccataga acgtgaaggg tttatagaag
     2161 cggccgccgt cgaggttatt gaacatgatg ccctcggccg gtgcgtaatc gacgctggcg
     2221 ctgctgctca cgatcacgat gcgcccggtg ccgactttca ccaggtcgag gatgccgttc
     2281 agcagtgcga agtgtccgat atgattgacc aggaactgca tctcgacgcc gtagcgagtt
     2341 tccaggaacg gcaaattggc gataccggcg ttggctacga ttgcgtcgag ctgatcgtga
     2401 agcgagcgaa tcgtgtcggc cgccgcggca accgaatcga gatccgcgag atcgcaggcc
     2461 accggaatgc ataaggggcc cgctttgcgg caagcaatct cggcgcgctc gagtgagcgg
     2521 gcgagaccaa tcacctgcgc accgttggcc gcgagcgcgc tcatcgtttc gaagccgagc
     2581 cccgaggtgc accccgttac cacaaagcgt ttgccggtga gatcgatgcc cgcaagcacc
     2641 tgatcggcgg tggagcgcat accgaatgga gtcgacttaa gcatcggcaa tacctctctg
     2701 caggcgtggg ttcagcggca cgcagaccat gcgcgcggcg gccgtcatgc cccgcaaaaa
     2761 ttcctctcgc gacgcgcaac tgtgcttgag accgcgcgct gttgcatgca gggcgcgcgc
     2821 caagtcggct gcgcgcaccc cgataaggcc gcatacctcg ccgagggccg agttggcaat
     2881 ggcgccggca acggccgctt cgaattgggt ctcgtagtgg gttagcgttg tgcccgcgag
     2941 cgaggtgctc gcgcacatca gatcggcggc atccgctccg agcgatccga caaagcgtcc
     3001 ggaccacgca tcgcaagcgg caattagccg cagttccagg ctctcatcgg cgcgctccag
     3061 ggcggcgctg gcggcgttca attgctggct gagcgtatgc accagcgctt tgcgaaacaa
     3121 ttcctccttg ttggcgaatt gcagatacaa gccttgccgc gacacaccgg cggcacgcgc
     3181 cacctcgtcc atcgatgtct tgcgaaaccc gaagcgggcg aatacggcca cggcggcatc
     3241 gagcgccggc agcaggcgca catcagtttg ttcgatctcc gggttgtgaa ttatctccac
     3301 tcctacacaa tagacaacgt tgggctatag tgtcaaatgc gtcacgacaa gatgggattc
     3361 gatagataag caaaacaagc agatacagtg ctttcataca ctacatatta atttaacaga
     3421 tggcaaaccg gtgcataggc tagtatcggc ggcgatcatg gatgcctgac tgcggtggac
     3481 ctcaaatccg atacgcatgg cctgccattt ccgccgggcg catcggcact catgaccgac
     3541 attccgcggc tgcgggccgg ccgcgttgca gggcaattct ggtcggtatg gataccgagt
     3601 ggcatgcaag gttttgaggc cgtgcaaacc acgctcgaac aaatggatct ggtcaagctg
     3661 atggccgccc gctacccgga tgatctcgag atggcgtaca gcgcggcgga cgtgcgccgc
     3721 atccaccggt cgcataaagt ggcctcgatg atcggcatcg agggcggcca tcaaatcaac
     3781 aattccctcg cggtgttgcg ccaaatgtat gacgccggtg cgcgctacat gactttgacg
     3841 cacacgctca ataccggctg ggcggattcg gcgaccgatg cgccgctaca tcacggcctc
     3901 acgcccttcg gcgtcgaagt ggtgcgagaa atgaatcgcc tcggcatgct ggtcgacctc
     3961 agccatgtat ccgccgagac catgcgcgcc gcactctcgg cgagcgcggc gcccgtgatt
     4021 ttctcccact cctcggcgcg cgcgctcgtc gatcatccgc gcaatgttgc cgacgacatt
     4081 ctgcggtcgg tcgccgccaa cggcggcgtc gtgatggtca attttgcgcc cggctatgtg
     4141 tcggaatccc gcaatcgctg ggacgccgat caggctgccg aacgggcgcg cttcaacagc
     4201 ccgccgtacg tcgggctcta catcggtcaa ccggagcggg cgaaagcagc ggtcgcagtc
     4261 tgggaagaaa atcacccacg gccggaggtt acgctcgcgc aggtggcgga tcatatcgat
     4321 cacatccggc aagtggccgg cgccgatcat gtcggactgg ggtcggactt cgacggcatc
     4381 gaggatgcgc ctgtgggact cgagggcgtg gaccgctatc cgctgctgct cgcggagctg
     4441 atgcgtcgcg gct
//
LOCUS       DASWZT010000261         2323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_16380, whole genome shotgun sequence.
ACCESSION   DASWZT010000261 DASWZT010000000
VERSION     DASWZT010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16380"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..731)
                     /gene="hutC"
                     /locus_tag="VGE92_07045"
     CDS             complement(12..731)
                     /gene="hutC"
                     /locus_tag="VGE92_07045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine utilization repressor"
                     /protein_id="HEY1313614.1"
                     /translation="MSRATVSLQPLYLQVKRHILDNIGSGKWGTSMRVPSENDIVKSF
                     GVSRMTANRALRELRDEGVLVRIAGVGSFVADQHAHAHPLEIRGIADEIRRRGHAHRA
                     EIISLERIRAVADLAEDFGIAARSELYCSIIVHFENDRPIQLEERYVLPKLAPDYLQV
                     DFNQTTPYDYLVKVAPLQEAEHLLRAVMPDERTRKLLAMKRDEPCLLMIRRTWTAGQI
                     ASVARLHYPGSRYELSGRFRP"
     gene            complement(764..2173)
                     /locus_tag="VGE92_07050"
     CDS             complement(764..2173)
                     /locus_tag="VGE92_07050"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF006681.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HEY1313615.1"
                     /translation="MARYSIQSAFLSQGWARDVGIVVSEEGFITEISTAAAATPELAG
                     GAERIEGIVVPGMANAHSHAFQRAMAGNTEFRLSARDSFWTWRRAMYALANRIDPDDL
                     QTLATQLFVEMLKSGYTSVAEFHYLHRQKDGVPYVGGNALWDAVSDAASIAGIGLTFL
                     PTLYQTSDFGGKPLKAEQSRFALDTDQFLRAVAERIAADKRSHLPAQRTGVAFHSLRA
                     VPLPTLVTVAQHLREIDAALPVHIHVAEQLLEVQACVRETGRRPIELLLDSGLLTPHW
                     CLVHATHATPAELDGIAAAAAAVCVSISTEANLGDGFFDTGRFLNAGGRLCVGSDSQS
                     TVNPAEELRWLEYQQRLRKKRRGVLAADSESHVGTRLWRDAAQHGARAIGQPAGVLEV
                     GRRADWLVLDAAHPSMVGAAADTALDHLLFAGGDAAIRDVMVAGRWVIKDHHHAAEVE
                     LRSRFAELMRTLATADQSG"
BASE COUNT          438 a          728 c          740 g          417 t
ORIGIN      
        1 tggttacatc ctcaggggcg gaaacgtccc gacaattcat agcgggaacc ggggtagtga
       61 aggcgggcca ccgaggcgat ttgcccggcg gtccaggtgc gtcgaatcat caacagacag
      121 ggttcatcgc gcttcatggc caggagcttg cgcgtgcgct catccggcat caccgcgcgc
      181 aacaaatgtt cggcttcctg cagcggcgcg actttcacca agtagtcgta cggagtggtt
      241 tgattgaaat ccacctgcaa atagtcgggt gcgagcttcg gcagaacata gcgttcctcc
      301 agctggatcg gccgatcgtt ttcgaagtgt acgatgatgg agcagtacaa ttcgctgcgc
      361 gctgcgatgc cgaaatcttc ggcaaggtct gcgaccgcgc gaattcgctc gagcgaaata
      421 atctcggcgc ggtgcgcgtg gccgcggcgg cgtatctcgt cggcgatgcc gcggatctcg
      481 agcgggtgtg cgtgggcgtg ttgatcggca acgaagctgc cgactccggc aatgcgcacc
      541 agcacgcctt catcgcgcaa ctcgcgcagc gcccgattgg cggtcatgcg cgacacgccg
      601 aaggacttca cgatgtcgtt ctcggacggc acgcgcatcg atgtgcccca tttgcctgag
      661 ccgatgttgt caaggatatg ccgcttcacc tgcagataca gcggctgcaa cgagacagta
      721 gccctactca tggcaggaga ttaacctgta tagacaggta cagtcaacca ctttgatcgg
      781 cagtggcgag cgtacgcatg agctccgcga agcgcgaccg caattcaacc tcggccgcat
      841 gatggtggtc tttgatcacc cagcggcccg ccaccatgac atcgcggatc gcggcatcac
      901 cgccggcgaa cagcaaatga tccaacgccg tatccgctgc ggcgcccacc atcgatggat
      961 gcgcggcgtc gagcaccagc cagtcggcgc gccggccgac ttcgaggact cccgccggtt
     1021 gtccgatggc gcgcgcgcca tgttgcgcgg cgtcacgcca tagtcgagta ccgacgtgcg
     1081 actcgctgtc cgcggcgagc acgccgcgcc gctttttacg caggcgctgc tggtattcca
     1141 gccagcgcag ctcctcggcg ggattcacgg tcgactggct gtcggagccc acgcacagcc
     1201 gcccgcccgc gttgaggaag cggcccgtgt cgaagaaccc atcacccaag ttcgcttcgg
     1261 tgctgatcga cacacaaacc gcggcagcgg ccgccgcgat gccgtcgagt tccgcgggcg
     1321 tcgcgtgcgt ggcatgcacc agacaccaat gcggcgtgag caatccgctg tcgagcagca
     1381 actcgatcgg gcgccggccg gtctcgcgca cgcaggcttg cacttcgagc aactgttccg
     1441 cgacatgaat gtgcaccggc aaagcggcgt cgatctcgcg caggtgctgc gccacggtca
     1501 ccagtgtcgg taagggcacc gcacgcaggc tgtggaaggc gacgccggtg cgctgcgccg
     1561 gcaagtgact gcgcttgtcg gcggcgatgc gctccgcgac ggcgcgcaag aattgatcgg
     1621 tgtccaaagc aaatcgcgac tgctctgctt tgagcggttt gccgccgaaa tcgctggttt
     1681 gatacagggt cggcagaaac gtgaggccga tgccggcaat cgatgccgcg tcgctgacgg
     1741 cgtcccacaa tgcgttccct cccacatagg gcacgccgtc cttctggcgg tgcagatagt
     1801 gaaattccgc gaccgaggtg tagccgctct tcagcatctc gacgaatagc tgcgttgcca
     1861 gggtctgcaa gtcatccgga tcgatgcgat tcgccagcgc gtacatggcg cgccgccagg
     1921 tccagaagct gtcgcgcgcg gacaatcgaa attcggtgtt gccggccatg gcgcgctgga
     1981 aggcatggct gtgcgcattg gccatgccgg gaacgacgat gccctcgatt cgttcggcgc
     2041 cgccggcaag ctcaggcgta gccgcagccg cggtgcttat ctcggtgatg aagccctcct
     2101 cggagacgac gatgccgacg tcgcgggccc aaccctgcga caaaaaggcc gattgaatgg
     2161 aatagcgcgc catgttgact atacctgtat agggaaccac tcttgccgta tcatacgtac
     2221 atcagtcagc ggtgggtacc aagtgccgaa atcttcgagc gatgcatggg accaggtttg
     2281 ggtgggcgcc cacatcgcca ccatggtcgc gggtgccgaa ccg
//
LOCUS       DASWZT010000262         5319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_69693, whole genome shotgun sequence.
ACCESSION   DASWZT010000262 DASWZT010000000
VERSION     DASWZT010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69693"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="VGE92_07055"
     CDS             complement(<1..728)
                     /locus_tag="VGE92_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY1313616.1"
                     /translation="MSETVASIVAAHGAGTVTPEQTLARSYARIRAHGDNAIFISLRA
                     EADVLAEARALAAAGHTGLPLYGVPVAVKDNIDVAGLPTTAACPAFSYNPGQDATAVA
                     RLRAAGAIVIGKTNLDQFATGLVGVRSPYGIPKNLFDPDFIPGGSSSGSGVAVAAGLV
                     PLALGTDTAGSGRVPAGFNNIVGLKPSLGLISTFGVVPACRTLDCVSVFALTVDDAWT
                     ALAAMAAPDSADAYSRNRAHGTPGA"
     gene            1164..2165
                     /gene="glsA"
                     /locus_tag="VGE92_07060"
     CDS             1164..2165
                     /gene="glsA"
                     /locus_tag="VGE92_07060"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEY1313617.1"
                     /translation="MDQKVGAAPAKRSELDNILSEAYSRHKPNKTGALADYIPELTRA
                     DPEGFGVAIATVGGGVHTIGDTDTPFTIQSVSKAFIYCLALELAGRDAVLARVGVEPS
                     GDAFNAIIFDPHTHRPFNPMVNAGAITVSGIVRDFAGAGAFDLILERLSEAAGRKLAV
                     DDAVYRSEAETGHRNRAIGHLLRNFGALEGSVDEVLDIYFRQCSILVTAADLACMGAT
                     LANIGQNPVTGRQVFDLAAVRSTLAVMFTCGMYDYSGHWAYDVGVPAKSGVGGGIVGV
                     VNRQLGIGTYSPRLDAKGNSVRGVDAFTELSNELGLHAFECSNVGSAYVRSFITGPV"
     gene            2248..2568
                     /locus_tag="VGE92_07065"
     CDS             2248..2568
                     /locus_tag="VGE92_07065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY1313618.1"
                     /translation="MQIDINDAGSTAIVTLVGRLDISGADVVALPLATLSGSKNNLVI
                     DMAGVTFVASIGLRHLVQAAKAVGRKGGRLVLLNPNTAVAEVVTTSGLAELLNIEHGS
                     APQS"
     gene            2593..4227
                     /locus_tag="VGE92_07070"
     CDS             2593..4227
                     /locus_tag="VGE92_07070"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HEY1313619.1"
                     /translation="MQAVAPFLTGLGLFFCGVHFVSSNLVPLAGRRFRALLTRLGRRP
                     WLAALFGSLAGVVTQSTNAVTSVIIGLVSGGLVDKRRAILIPTWAHVGTSVLVILVAV
                     DFRVAASYLVAITGCAVYFGFDRNDRVRYAVGTLLGLGLLFLGMTMLKSGAEPLRDYL
                     VAEGLMASAAKAPLFLLGLGAGLSLLCQSSSVAGALAVAGSSAGLVDLSGACWLIYGA
                     NLGSGANYALLAKTHRGEAAQIALMQVAQKLAGFTVVLLIMLVERVTGRLLIESAAAA
                     LSPSLSHEVAWVFLFYQLAGSTVCTAFVGPILTLLERAAPPSNLQELSKPAYLIEDAL
                     VEPSFAIELVGREEQRLLQRLPTMLDGVRADAEGEPIPAPVLRTAGAAVTRAMASYLE
                     SINEGSLDRADRERVVRLQHRAANLNAMHESLEEFVSACLVARQWPSSGRVADQMVES
                     LHALLFALGDAATSADPADQELVLSLLGHRDEMMERIRQRVLREDPNMPPNPQEALFA
                     ATMLFERVVWLARRSAILLTPERGSARNSAATGSAG"
     gene            4224..>5319
                     /locus_tag="VGE92_07075"
     CDS             4224..>5319
                     /locus_tag="VGE92_07075"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HEY1313620.1"
                     /translation="MIDPKARILVVDDVAENRDLLLRRLTRLGFSLIDQAANGVEALA
                     AIGEKSYDLVLLDIMMPELDGFGVLEALRADGRVNDLPVIVISALNEIDPVVRCIELG
                     AADFMFKPFNPTLLRARVLTTLEKKALTDRTRNELKRKQYELNEARTLQLALAPPPFA
                     GKLSGRSLSIDVLLEPAKEVGGDLVDHFRVGDDLVVLALGDVSDKGAGAALMMARTHA
                     MLRALTARPDAASLFREPQNAVGIVNDALAEGNASCMFVTFLLASFDLTSGRLAYVRA
                     GHIPPFHRNGTGKVARLGRMGGPPLGLIETTKYQADTVTLAPGDRLLIVTDGFTEAHD
                     PGGSMYGEARIEEFLTALAPSEAEPLKRLAG"
BASE COUNT          952 a         1677 c         1652 g         1038 t
ORIGIN      
        1 gcaccgggcg tgccgtgcgc gcggttgcgc gaataggcgt cggccgaatc cggtgctgcc
       61 atggccgcaa gcgccgtcca ggcatcgtcg acggtcagcg caaacaccga cacgcaatca
      121 agcgtgcggc aggcaggcac cacgccgaag gtcgagatca agccgaggct tggtttcaga
      181 cccacgatat tattaaaacc cgccggtacc cggccggagc cagccgtatc ggtgccaagc
      241 gcgagcggga ccagaccggc cgcgaccgca acacccgaac cggaactcga tccccctgga
      301 ataaagtcgg gatcgaaaag attttttggg attccatagg gtgagcgaac gccgacgagg
      361 ccggtggcga actgatcgag attggttttg ccgatgacga tggcgcccgc cgcacgcagc
      421 cgcgccaccg cggtcgcatc ctgccccggg ttataactaa aggccggaca cgctgccgtc
      481 gtaggcaggc ctgcgacgtc gatattgtcc tttaccgcga cgggaacgcc gtaaagcggc
      541 agcccggtgt ggcctgcagc ggcgagcgca cgggcttccg ccagcacgtc ggcctccgcc
      601 cgcaggctga taaagatggc gttgtcgcca tgcgcgcgga tgcgggcata actgcgggca
      661 agggtctgct ccggcgttac cgtccccgcc ccgtgggcgg ctacgatcga ggcgacggtc
      721 tcggacaaaa cgttccccct acggcggctc agctggctga tgcagcagcg caggtgtccc
      781 cttgctctgc cgacttccct gctaacaggg aatcttacag gaaattcccc caggccgttg
      841 cagcgatttt gatgcgattt ccttactcga tgtaacaggg aattaatttg cttgaacagg
      901 gaaaattgtc gcgacagcag ggaattttat gagcagaatc tggatccacc gccggatgag
      961 attttctcca tgaagaatgc aggcactgtt cgctattttc cccggcgagc ggttcggaaa
     1021 tctcgcgata cacaccgtgc ccatcgggga gttcgacgga ccatattcgt tcttcaatag
     1081 cagtggaaaa ttgctctaat tttttgcagc atagctaccc atcatctgtg gccgatccgg
     1141 tgatattccg ctaaaaaaga acgatggacc agaaagtcgg cgccgcgcct gcaaagcgat
     1201 ccgaactcga caacatcctt tccgaggctt attcgcgcca taagccgaac aagaccggcg
     1261 cgctcgcaga ctacattccc gagctgacgc gtgccgaccc ggagggcttc ggcgtcgcca
     1321 tcgcgacggt cggcggcggc gtccatacca tcggcgatac cgacacgccg ttcaccatcc
     1381 agtcggtgtc caaggcgttc atttattgcc tggcgctgga attggctgga cgcgacgcgg
     1441 tcctggcacg cgtcggcgtg gaaccgagcg gggacgcctt caacgccatc atattcgatc
     1501 cgcacaccca ccgtccgttc aatccgatgg tgaatgccgg cgccatcacg gtcagcggga
     1561 tcgtgcgcga tttcgcgggc gccggcgctt tcgacttgat cctcgaacgg ttgtcggaag
     1621 cggcgggccg caaacttgcg gtcgacgacg ccgtctaccg gtcggaagcc gagaccggcc
     1681 accgcaaccg ggcgatcggc catctgctgc gaaatttcgg cgccctcgaa gggtcggtcg
     1741 acgaagtgct cgacatttat ttccgccaat gttcgattct ggtgaccgcc gccgacctcg
     1801 cctgcatggg cgcgacgctg gccaatatcg gccagaaccc ggtgaccgga cgccaggtgt
     1861 tcgatctggc agcggtgcgc agcacgctcg ccgtgatgtt cacctgcggc atgtacgact
     1921 attccggcca ctgggcctat gacgtcggcg tgccggccaa aagcggcgtc ggcggcggca
     1981 tcgtcggcgt ggtcaaccgg cagctcggta tcggtaccta ctctccccgc ctcgacgcga
     2041 agggcaattc ggtgcgtggc gtggacgcct ttaccgagct ttcaaacgaa ctcggtctgc
     2101 atgcctttga atgctcgaat gtcggatcag cctatgttcg ttcgttcata acggggccgg
     2161 tctgataatg gttttttggc tggctgcccc tgtcaccaaa ctgtcataca agcttgtttt
     2221 agtcatcgcg ggctcgggga agcactcatg caaatcgata tcaatgatgc aggatcgacc
     2281 gcaatcgtaa ccttggtcgg gcggctcgat atatcggggg cggacgttgt tgcgttgccg
     2341 ctcgccacgc tctcgggcag caaaaacaac cttgtgatcg acatggccgg ggtgaccttc
     2401 gtcgcctcga tcggtctgcg tcatttggtg caggccgcca aagcggtcgg acgcaagggc
     2461 ggccgtctcg tgttgctcaa cccaaatacc gctgtcgccg aagtcgtgac gacctccggc
     2521 ctggctgaat tgctcaacat cgaacatggc agcgccccgc aaagctaagt tggcgcaagg
     2581 taggcggggc tcatgcaagc ggtcgcgccg ttcctgaccg gtctcgggct ttttttctgc
     2641 ggcgtccatt tcgtttcctc aaatctcgtt cctctggccg gccgccgctt ccgagcactg
     2701 ctgacgcgcc tgggacggcg tccttggctg gccgctctct tcggctctct cgccggcgtc
     2761 gtcacgcaga gcacgaacgc cgtcacctcg gtcatcatcg gcctagtgag cggcggcctg
     2821 gtcgataaac ggcgcgccat cctgattccg acatgggcgc atgtcggcac gtcggtgctc
     2881 gtcattttgg tggcggtcga tttccgggtg gcggccagtt atctggtggc gataacggga
     2941 tgcgcggtgt atttcggttt cgatcgcaat gaccgcgtcc gctacgcagt cggaacgctg
     3001 ttgggtctcg gcctgctttt tctcggcatg acgatgttga agtcgggcgc agaaccgctg
     3061 cgcgactacc tggtcgcgga aggcctgatg gcgagcgcgg ccaaggcgcc cttgtttctg
     3121 ttaggccttg gcgccggatt atccttgctg tgccaatcct cgtcggtcgc cggcgcgctg
     3181 gctgtggccg gcagcagcgc cggcttggtc gacctctccg gcgcgtgctg gctgatctac
     3241 ggcgccaacc tcggctccgg cgcaaattat gcgttgctgg caaagaccca tcgcggcgag
     3301 gccgcgcaaa tcgcattaat gcaggtcgcg cagaagctcg ccggctttac cgtcgtgtta
     3361 ctgattatgc tggtcgagcg cgtcaccggc cgcctcctga tagagagcgc ggctgcagcg
     3421 ctcagcccct cactttctca cgaagtggct tgggtatttc tgttctatca attggccggc
     3481 tcaacggtgt gtacggcgtt cgtcggaccg atcctcactt tgctcgagcg cgcagcgcca
     3541 cccagcaatc tccaggaact ctcaaagccg gcttatctga tcgaggacgc gttggtcgag
     3601 ccgtccttcg ccatcgaact ggtcggccgc gaagaacagc gcctgctgca gcgcctgccg
     3661 acaatgctcg atggcgtgcg tgccgacgcc gagggcgagc cgatcccggc cccggtgttg
     3721 cggaccgccg gcgcggccgt cacccgcgcc atggccagct atctcgaatc catcaacgaa
     3781 ggcagcctgg accgcgccga ccgggaacgc gtcgtgcgcc tgcaacaccg cgccgccaac
     3841 ctgaatgcga tgcatgagag cctagaggaa ttcgtcagtg cctgccttgt agcgcggcag
     3901 tggccatcga gcggccgcgt cgccgaccag atggtagaat cgctgcacgc cttgctgttt
     3961 gcgctcggtg atgccgccac atccgccgac cccgctgacc aagaacttgt cctgtcattg
     4021 ctgggacatc gcgacgagat gatggagcgg attcgccagc gcgtgctgcg cgaggacccc
     4081 aatatgccgc cgaacccgca ggaggcattg tttgccgcca caatgctgtt cgaacgggtg
     4141 gtctggcttg cgcgccgcag cgcgatcctg cttactcccg agcggggtag cgcgcgcaat
     4201 agtgcggcga cgggatctgc gggatgatcg atccgaaggc tagaatcctg gtcgtagacg
     4261 acgtcgccga gaaccgcgac ctgctgttgc gccggctcac gcgtctcggg tttagcctga
     4321 tcgatcaggc ggccaacggc gtcgaggcgc tcgcggcgat cggtgaaaaa tcctacgacc
     4381 tcgtactact cgacattatg atgccggagc tcgacggttt cggcgtgctc gaggcgttgc
     4441 gcgccgacgg ccgcgttaac gacctgccgg tgatcgttat ttcggcgcta aacgaaatag
     4501 atccggtggt gcgctgcatc gagttgggcg ccgccgactt tatgttcaag ccgttcaatc
     4561 cgacgctact gcgcgcacgc gtgctgacca cattggaaaa gaaggccctc actgatcgca
     4621 cgcgcaacga actcaaacgc aaacaatacg aacttaacga agcgcgcacg ctccaattgg
     4681 cgctggcgcc gccgccgttt gcagggaaac tgtccggccg gtcgctgtcg atcgacgtgc
     4741 tgctcgagcc ggccaaagaa gtcggcggcg acctggtcga tcacttccgc gtcggcgacg
     4801 acctggtcgt gctggcgctc ggagacgtat ccgacaaggg tgccggcgcc gcgctgatga
     4861 tggcgcgcac gcatgcgatg ttgcgggcgc tcaccgcccg cccggatgcc gcctcattgt
     4921 ttcgtgaacc gcagaatgcc gtcggcattg tgaatgatgc gctcgcggaa ggaaacgcga
     4981 gctgcatgtt cgtcaccttt ttgctggcgt ctttcgacct caccagcgga cgcctcgctt
     5041 acgtacgcgc agggcatatt ccgccattcc atcgtaatgg caccggtaag gtggcgcgcc
     5101 tcggacgcat gggcgggccc ccgctcggcc tcatcgagac cacgaagtac caggccgata
     5161 cagtcacgct cgctccgggg gaccggctgc tgattgttac cgacggcttc accgaggcgc
     5221 acgatcccgg cggttcgatg tatggcgaag ccaggatcga agagttcctg accgctcttg
     5281 cgccctcgga agccgaaccg ctcaaacggc tggccggcc
//
LOCUS       DASWZT010000263         3515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_2010, whole genome shotgun sequence.
ACCESSION   DASWZT010000263 DASWZT010000000
VERSION     DASWZT010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2010"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VGE92_07080"
     CDS             <1..819
                     /locus_tag="VGE92_07080"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY1313621.1"
                     /translation="EPLVLIHGLGCSGADWAFQVAALEKQFRVIVPDLPGSGHSPPLE
                     NYSIAGFAAAIWALLDHLGVARANVVGFSLGGAVALEMAVQRPAAVPRLGLINSLATY
                     LPSDWRKWLEVYVSATLVRTFGMQRTAWLLALRLFPEPWQRAMRKHSAAVVGAVPATS
                     YLGMGFALARWAILDRLDRLQCRTLLIAAEHDFTPLAEKVALAARLKADIVVVRGSRH
                     GTPFDSVETTNASLLALLTDRPLPPAAPRLRDTPTRAQALSLAGSIAEEHALSI"
     gene            865..1923
                     /locus_tag="VGE92_07085"
     CDS             865..1923
                     /locus_tag="VGE92_07085"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEY1313622.1"
                     /translation="MQNLRLDVRLDTSLELLLERAPATPGDRVLATVVATAGSTYRKP
                     GARMLIMADGSYLGLLSGGCLEADLKLHARQVLDDGVPRAIEYDMRGPDDILFGIGAG
                     CEGAMRVLLEPAALGSHAAAALAAAGAATQAGRPTVLVSVHESVDFPLGTYVAAAPLP
                     PALLDAAALSLATRAQMTQDGSGLRAFVQFLAPPPHILICGAGPDAQPVVGAARTLGW
                     RVSIVDHRPAYAQPGDFPGATVKLCDPRSLRSAVDLEQCHAAVVMSHHLQSDAMYLRE
                     LAAAGKPAYIGLLGPEARRKRLARDLAGEAQRLRCRVLGPVGIDIGAVTPEGIALSII
                     GQIHAWLAGRGGVVAAEL"
     gene            2086..>3515
                     /locus_tag="VGE92_07090"
     CDS             2086..>3515
                     /locus_tag="VGE92_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY1313623.1"
                     /translation="MFKLLTLSSVALSAALAACGGSSSPTATVAASTARGTLALNPPF
                     RIASLTAAALQAKLAANSSSAELLKRAGDPNCGVDFYYVKFWTLGGAATPEVTESSGA
                     LMVPTGAAPACSGPRPIVLYAHGTNPDKALNIADITNASNTEGVLIAAVFAAQGYIVV
                     APNYAGYDISTLGYHPYLNAAQQSGEMIDMLAAARTALPKTLSAATSDSGKLFITGYS
                     QGGYVAMATQRALEASGAVITGAAPASGPYALEAFGDVLIFGNVDLGSTVFTPLLTTS
                     YQKAYGNLYSTPSDVYAPAYAAGIETLLPSVTPIDTIFADGLLPETAEFDSTTPVVSI
                     PGNPTLSLQLTALLSVPGSPQYPLPPTAQTPLFALGFGASYLVNNSYRLSYALDAAAD
                     PDGAVPTPSAGVKLAATEPVQPLRQAFYKNDLRNGAWSPNSPTLLCGGDQDPTVFFSV
                     NTGTMAAFWSPLVQAGLITVLDVNATP"
BASE COUNT          577 a         1157 c         1139 g          642 t
ORIGIN      
        1 gagccgctgg tgctgattca cggactcgga tgcagcggtg cggattgggc attccaggtt
       61 gcggcgctcg agaagcagtt tcgcgtcatc gtccctgatc tgccgggaag cggccacagc
      121 ccgccgctcg agaattacag cattgccgga tttgcggcgg cgatttgggc gctgctcgat
      181 cacctcggtg tggcgcgagc caacgtggtt gggttttcgc tgggcggcgc agttgcgctc
      241 gagatggcgg tgcaacgtcc cgctgccgtg ccacgcctcg gactcatcaa cagcttggcc
      301 acgtatcttc cgagcgattg gcgtaaatgg ctggaagtgt acgtctcggc gacgctggta
      361 cgtacgttcg gcatgcagcg cacggcttgg ttgctggcgc tgcggctgtt tcccgaaccc
      421 tggcagcggg ccatgcgcaa acacagtgcg gccgtggtcg gtgcggttcc cgcgacctcg
      481 tacctcggca tgggatttgc gctcgctcgc tgggccatcc tcgatcgcct cgaccggctc
      541 caatgccgca cgttgttgat tgcggccgag catgatttca ctccgctggc ggagaaggtc
      601 gccttggccg cgcggctcaa ggccgacatc gtcgtggtgc gcggctcgcg tcacggcact
      661 ccgttcgact cggtcgaaac caccaatgcc agcttgctgg cgctgctcac ggatcggccg
      721 ttgccgcccg ccgctcccag gctgcgggac acgccgacgc gggcgcaggc gctgtcgttg
      781 gccggaagca tcgccgagga acatgccttg agcatttgag tttttaccta ccgctgagcc
      841 cgccgttttg aaacaatgcc ggctatgcaa aacctgcgcc tcgatgtgcg gctcgacacc
      901 agtctcgaat tgcttctgga acgcgcgccg gcaacacccg gtgatcgcgt gctcgcaacc
      961 gtggtggcga cggcgggctc gacctatcgc aaacccggtg cgcgcatgct catcatggcc
     1021 gacggcagct acttgggatt gctcagcggc ggctgtctcg aggccgatct caagctgcac
     1081 gcccgacagg tgctggacga cggtgtgccg cgcgccatcg agtacgacat gcgcgggccc
     1141 gacgatatcc tgttcggcat cggcgccggg tgtgagggcg cgatgcgtgt attgctcgaa
     1201 ccggcagcgc tcggcagtca tgcggccgcg gcgctcgcgg ccgcaggcgc cgccacgcaa
     1261 gcgggacggc cgacagtgct ggtgtcggtg cacgagtctg tcgattttcc gctcggaacc
     1321 tatgtcgccg cagcgccgct gccgcccgcg ctgctcgacg ccgccgcact gtcgctcgca
     1381 acgcgtgccc agatgacgca ggacggctcg ggcctaaggg cattcgtgca atttctcgca
     1441 ccgccgccgc acatcctcat ctgcggagcg ggacccgatg cgcagccggt ggtcggtgcc
     1501 gcccgcaccc tcggctggcg cgtgtcgatc gtcgaccatc gaccggcata cgcgcagccg
     1561 ggcgacttcc ccggcgcaac cgtcaaattg tgcgatccgc gctcgctgcg ctcggcggtc
     1621 gacctcgaac aatgccatgc agccgtggtc atgagtcatc acctgcaatc cgatgccatg
     1681 tacttgcggg aactcgccgc ggccggaaaa cccgcctaca tcgggctgct gggtccggag
     1741 gcgcgtcgca aacggctcgc gcgcgacctg gcgggcgagg cgcagagact ccgatgcaga
     1801 gttcttggcc cggtgggcat cgatatcggc gcagtgacgc cggaaggcat tgctctgtcg
     1861 attatcggcc agattcacgc atggctcgcc gggcgcggcg gcgtagtggc cgcagagctc
     1921 tgagaaccgg ttacagcgtc ggccgccgcc gtcaagggga tgcctgtcgg tgcgggttgg
     1981 cgtagactca agggcaattg cgtgccggca tatatctaac aaacacgaca ttggcacgtc
     2041 cggcggcgta gtaaattcgt gcagagattc gggagacaga cagccatgtt caagttactg
     2101 acgctttcga gcgtggcgtt gagcgctgcg ctcgccgcgt gcggcggatc gagctcgccg
     2161 actgcgaccg tcgccgcgtc cacggctcgc ggcacgctgg cgctcaatcc accgtttcgt
     2221 atcgcctcgc tcacagccgc ggcgctgcaa gcgaaactcg cggcaaacag ctccagtgcc
     2281 gagctcttga aacgcgccgg cgaccccaat tgcggtgtgg atttttacta tgtgaagttc
     2341 tggaccttgg gcggcgccgc gaccccggaa gttaccgagt catcgggcgc cttgatggtg
     2401 ccgacgggcg ccgcgccggc gtgttcgggg ccgcgtccca tcgtgctgta tgcacacggc
     2461 acgaacccgg acaaggcgct caatatcgcc gacatcacca atgcgtcgaa taccgaaggt
     2521 gtactgatcg cagccgtgtt cgccgcacag ggctatatcg tggtggcacc gaattacgcc
     2581 ggctacgaca tttcgacatt gggctaccac ccgtacttga atgccgcgca gcagtcggga
     2641 gaaatgatcg acatgctggc ggcggcacgc accgcgttgc ccaagacctt gagcgccgcg
     2701 accagcgaca gcggcaagct gttcatcacc ggctactcgc aaggcggcta cgtcgcgatg
     2761 gcgacgcagc gggcgctcga ggcgagcggt gcggtcatca cgggcgccgc gcccgcttcc
     2821 ggaccgtatg cgctcgaagc gttcggcgat gtgctcattt tcggcaacgt cgacttgggc
     2881 tcgacggtat tcacgccgct gctgacgacc agctatcaaa aagcctacgg taacctctac
     2941 agcacgccga gcgatgtgta cgccccggcc tatgcggccg gcatcgagac cttgctgccg
     3001 agcgtcactc cgatcgacac cattttcgcc gacggtcttc tgcccgagac cgccgaattc
     3061 gacagcacca cgccggtggt gtcgataccc ggcaatccga cgttgtcgct gcagctgacg
     3121 gcactcctct cggtgcccgg cagcccccaa tatccgctgc cgccgacggc gcagactccg
     3181 ctgtttgcac ttggattcgg tgcatcgtac ctggtcaata actcctatcg cttgagctac
     3241 gcgctcgacg cggccgcaga tcccgacggt gcggtgccga cgccgagtgc gggcgtcaag
     3301 ctcgccgcaa ctgagccggt gcaaccgctg cgccaggcct tttacaagaa cgatctacgc
     3361 aacggggcgt ggagcccgaa ctcgccgacc ttgctgtgcg gcggcgacca ggatccgacg
     3421 gtattcttct cggtgaacac cggtaccatg gcggcattct ggagcccgtt ggtgcaagcc
     3481 ggtctgatca cggtactcga tgtgaacgct acgcc
//
LOCUS       DASWZT010000264         4556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_11766, whole genome shotgun sequence.
ACCESSION   DASWZT010000264 DASWZT010000000
VERSION     DASWZT010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11766"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VGE92_07095"
     CDS             <1..1011
                     /locus_tag="VGE92_07095"
                     /inference="COORDINATES: protein motif:HMM:NF014804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY1313624.1"
                     /translation="EPLPKSQSAIVRQGCAIEARLYAEDPAHGYLPSVGRITHLRWPE
                     ASPGLRLDVGVDAGDEVSSYYDPMLGKLIAWSDSRGQAADRLRQALAALEILGVTTNR
                     ALLVSVLADAEFLRGGVATNFLGARAAHLSFGEPGAGDIDAVLAALWCATRCTAGDAL
                     WGDTRGWRLAAAPTSSWSFGDRTVSVEAGAPHRYRARLGTHEYALCIAARDADSLHVQ
                     VGAQMQHVRVVEVDQELHLFRDGRQVALHLRRTEDALAVSAAAEQGSLLTPLPGTVVA
                     VHVSAGQHVVRGAPLVTVEAMKMEHTLSAPYDGTVTRLAFGLAERVAAGAILVELAPR
                     EA"
     gene            complement(1008..2675)
                     /locus_tag="VGE92_07100"
     CDS             complement(1008..2675)
                     /locus_tag="VGE92_07100"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1313625.1"
                     /translation="MFISLKRHEREKQDLLGASTRLTDAIFKGSSQGLCLLDSKDKIL
                     TQISNSLSTLFRRQDFANLSFEKLIAPVVSAKSLSIARTYVNKLLDTAVATEDMPANP
                     LQDIEVRLANGDGSFATVHYCFEFNAVHLPREPRAWLVRITDITARVQQVRELEDLRT
                     QVQTQGEILRGVLMGGGARFGAFLQRTDASMRAINGVLKKPARQADAFRNKLEETLDE
                     VDRVRRDAAAFKLTGLEAAARLLEDALQELRSRGALSGSDFLPLAVKLDQLYGEFALL
                     RTLTTQASPVVQADEAQAEPRITDNGTAIIQAPKFIAESEQPSALAKAQHTAQAGSLD
                     STLQGLTELVAQELHKTVVLETAGLQLVPPRYQATIKNVAIQLIRNAVMHGIESSAAR
                     AAAAKPAHGTLHLEFKVLPDQSYELHFQDDGCGLDADKVRATAVAKGVIDAEAASRMR
                     DRQAIKLIFKSRYTTLEAVPGEAPHGTGMAMVRRYIHESGGRIALATLLGHETRFKIT
                     LPSLATTDALLSGEAQEPSNALQSMDVQEPTNSLPDDAQQHPDQRVA"
     gene            complement(2778..4061)
                     /locus_tag="VGE92_07105"
     CDS             complement(2778..4061)
                     /locus_tag="VGE92_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY1313626.1"
                     /translation="MPDRTDPILLVVGGDTKRLQWLTHHVTSHWPNAQVTTVPAPDPA
                     ALNQLLTERPPDAVILQADFADEAASAGALQCLTQLLQQRPTLHCIVLAENGNELTAV
                     RAMKAGAKEYLPLTRITRDQLLAAINDIWSKRRAALEAAKEATKALNQSGSEGSGVEV
                     PGYSIVKDIATSNFSQVFLARSERLRRNVVLKVMNRGESQRELDDAERFQREYEIISS
                     ITHRAIAEIYDFGSLPRHLYLAMEYFPCGDLRDRLRNPMSVDESLYYLRSIAEALRVI
                     HVFGILHRDLKPANVMLRDDNSPVLIDFGLARRAMEDSGTTGIGQVLGSPYYISPEQS
                     QGQRVDARTDLYSLGVMFYEMLTGQKPYTGKTALAIMTQHSTEPVPTLPPVLAPQQPL
                     LDRLMAKRPNSRYFSVDELLADLGPAIPTAALTVT"
     gene            4231..>4556
                     /locus_tag="VGE92_07110"
     CDS             4231..>4556
                     /locus_tag="VGE92_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313627.1"
                     /translation="MGSIRTWSLALVAGVFVVVVAHQRSHADAGPAFGPAAEPLTSTA
                     PAAGRVTGLPDFSGLVAENGAAVVNISVVEKAAKSANPGDPSDADDPLSQFFRHYQMP
                     NPERAPP"
BASE COUNT          750 a         1427 c         1523 g          856 t
ORIGIN      
        1 gagccgttgc cgaaatctca gagcgccatc gtgcggcagg gctgtgccat cgaggcgcgt
       61 ctgtatgcgg aggatcctgc gcatggatat ctgccgagcg tcggccgcat cacgcacctg
      121 cgctggcccg aggcctcgcc cggcctgcgc ctggacgtag gcgtcgacgc cggcgatgag
      181 gtctcgagct actacgatcc catgctcggc aagctgatcg cgtggagcga ctcgcgcggc
      241 caagcggccg atcgcttgcg tcaggcgctc gccgcgctcg aaatcctggg cgtcaccacc
      301 aatcgcgcgc tgctcgtgag cgtgcttgcc gatgctgagt tcctgcgcgg cggcgtcgcc
      361 acgaattttc tcggcgcgcg cgccgcgcat ctgtcgttcg gcgaacccgg cgccggcgat
      421 atcgacgccg tgctcgcggc cttgtggtgc gcgacccgat gcaccgcggg cgatgcgctg
      481 tggggcgaca ctcgcggctg gcgcctggcg gcggcgccca cgagttcctg gtctttcggc
      541 gatcgcacgg tgagcgtcga ggcgggcgcg ccgcaccgct atcgcgcgcg cctcggcacg
      601 cacgagtacg cgctctgcat cgcggcccgc gatgcagact cgttgcacgt gcaggtcggc
      661 gcacagatgc agcatgtgcg cgtcgtcgag gtcgatcagg agttgcacct gttccgcgac
      721 ggcaggcagg ttgcgttgca cctgcgccgc accgaagatg cgctggcggt cagcgccgcc
      781 gcggaacaag gatcgctgct cacgccgctg ccgggcacgg tggtcgcggt gcacgtgagc
      841 gccggccaac acgtcgtgcg cggcgcgccg ttggtgaccg tcgaggccat gaaaatggaa
      901 cacaccttga gcgctccgta cgacggtacg gtcacgcgcc ttgcattcgg cttggcagag
      961 cgggtggctg ccggggcaat cctggtggaa ctcgcgccgc gcgaggctta agcgacgcgc
     1021 tggtccgggt gttgttgcgc gtcgtcgggc agcgaattgg tgggttcctg cacgtccatc
     1081 gactgcagcg cattcgacgg ctcttgcgcc tcgccgctca acagcgcgtc ggtagtggcc
     1141 aacgacggca gtgtgatttt gaatcgagtc tcgtgaccga gcagggtggc gagcgcaatg
     1201 cgtccgccgg attcatggat gtagcgccgc accatggcca tgccggtgcc atgcggcgct
     1261 tcgcccggga ccgcctccaa ggtcgtgtag cgcgacttga aaatcaattt gatggcctgt
     1321 cggtcgcgca tccggctcgc cgcctcggcg tcgatcacgc ccttggcaac cgcggtggcg
     1381 cggaccttgt ccgcatcgag gccgcagccg tcgtcctgaa agtgcagctc gtagctctgg
     1441 tcgggtagaa ccttgaattc caagtgcaag gttccatgcg ccggcttggc cgcggccgcg
     1501 cgcgccgccg agctttcgat gccgtgcatc acggcgttac gaatcagctg aatggcgacg
     1561 ttcttgatgg tggcctgata gcgcggcggc accagctgca ggcccgcggt ttcgagtacc
     1621 acggtcttgt gcagttcttg ggccacgagt tcggtgagcc cctgcagcgt gctgtcgagg
     1681 ctgccggcct gggccgtgtg ctgcgccttc gcgagcgcag atggttgctc cgactcggcg
     1741 atgaatttcg gcgcttggat gatcgcagtg ccgttatccg tgatccgcgg ctccgcttga
     1801 gcttcatcgg cctgcacgac gggacttgcc tgcgtggtga gggtgcgcag cagcgcaaac
     1861 tcgccgtaca gctgatcgag cttgacggcg aggggcagaa agtcgctgcc gctcaaggcg
     1921 ccgcggctgc gcaattcctg cagcgcgtcc tccagcaggc gtgccgccgc ttcgagaccg
     1981 gtgagcttga atgccgccgc gtcgcggcgc acacgatcga cctcatcgag cgtttcttcg
     2041 agcttgttgc gaaacgcgtc cgcctggcgc gccggcttct tcaacacgcc gttgatcgcg
     2101 cgcatcgagg cgtcggtgcg ctgcaggaac gcgccgaagc gcgcgccgcc gcccatcaat
     2161 acaccgcgca agatctcgcc ctgggtctgc acttgggtgc gcaagtcctc gagttcgcga
     2221 acctgctgta cgcgcgcggt aatgtcggtg atgcgcacca gccaggcacg cggttcgcgc
     2281 ggcagatgca cggcgttgaa ttcaaaacag taatgcaccg tggcaaagct gccgtcgcca
     2341 ttcgccaagc ggacttcgat atcctgcagc gggttcgccg gcatgtcctc ggtggcgacc
     2401 gcggtatcga gcaatttatt cacataggtg cgggcgatgc tcagactttt cgcggacacc
     2461 accggtgcga tgagtttctc aaagctcaag ttggcgaagt cctggcgccg aaacagcgtg
     2521 ctcagcgagt tcgaaatctg cgtcaggatc ttgtctttgg agtcgagcag gcacaggccc
     2581 tgcgagctgc ccttgaatat ggcatcggtc aggcgggtgc tcgcgcccag caggtcctgc
     2641 ttctcgcgct cgtgacgttt gagggaaatg aacatgtatc cggagattgc caaacgcctg
     2701 ccgcagcggg attgatggcg ttcacagtag gatcggacag ttacatatct cgcgcgaagt
     2761 taaccggcga ctgagactca ggtcacagtc agcgcggcgg tcggtatcgc tgggccgaga
     2821 tcggcgagca actcatcgac cgagaagtag cgggaattcg gccgcttcgc catgagccgg
     2881 tcgagcagcg gttgctgcgg cgccaacacg ggcggcaggg tgggcacggg ctcggtgctg
     2941 tgctgggtca tgatggccaa cgccgtcttg ccggtatagg gcttctggcc ggtgagcatc
     3001 tcgtagaaca tgactcccaa actgtacagg tccgtgcggg catcgacgcg ctgcccttgc
     3061 gactgctccg ggcttatata atacggcgag ccgagtacct gaccgatgcc ggtggtaccg
     3121 gagtcctcca tcgcgcgccg cgccaaaccg aaatcgatga gcaccggtga attatcatcg
     3181 cgcaacatga cgttcgccgg cttcaagtcg cgatgcaaga tcccgaacac gtggatgacg
     3241 cgcaacgcct ccgcgatgct gcgcagatag tacagactct cgtcaacgct catgggattg
     3301 cgcagccggt cgcgcaggtc gccgcacggg aaatactcca tcgccaggta cagatgacgc
     3361 ggcaaggacc caaaatcgta gatctcggcg atggcgcgat gggtgatgct cgagatgatc
     3421 tcgtattcgc gctggaagcg ttccgcgtcg tcgagttcgc gctgcgattc gccgcgattc
     3481 atcaccttga gtacgacgtt gcggcgcagg cgctcgctgc gtgccagaaa aacctgcgag
     3541 aaattgctgg tcgcgatgtc cttgacgatg gaatagccgg gaacctcgac gccgctcccc
     3601 tcgctgccgg attggttcag cgccttggtg gcctccttgg cggcttccag ggccgcgcgg
     3661 cgcttcgacc agatgtcgtt gatcgccgcc aacagttgat cgcgggtaat gcgcgtcagc
     3721 ggcaaatact ccttggcgcc ggccttcatg gcgcgcaccg ccgtcaattc gttgccgttc
     3781 tcggcgagca ctatgcagtg caaggtgggc cgctgctgca gcaactgggt caggcactgc
     3841 agcgcgccgg cggacgccgc ttcgtcggcg aaatccgcct gcaggatcac ggcgtccggc
     3901 gggcgttccg tcagcagttg gttcagcgca gcagggtccg gcgccggcac ggtggtgacc
     3961 tgggcgttcg gccaatgact cgtcacgtgg tgcgtgagcc attgcaggcg cttggtgtcg
     4021 ccgcccacca ccagcaaaat tggatcggtc cggtctggca tagatcgagt ctggcactag
     4081 gaacgcgcag cgtagcgtgt gaaatcaggg ttggcccgtc gggaattcac atttcccgcc
     4141 ccttgagcgg aaaattgaaa ccataccccg ttgcgggagt cttagaatat ccggaatatc
     4201 cgcagagtgt gcaagagagg tcgtatttca atgggtagca tcagaacttg gtcgctggca
     4261 ctggttgcgg gagtcttcgt tgtggttgtc gcgcatcagc gatcgcatgc cgatgcgggc
     4321 cccgcgtttg gcccggcggc ggaacccctg acctcgactg cgcccgctgc cggccgcgtc
     4381 acggggttgc cggacttctc ggggctggtc gccgaaaacg gtgccgccgt ggtcaatatc
     4441 agcgtggtcg aaaaggctgc caaatcggca aacccgggcg acccgagcga tgccgacgac
     4501 ccgctgtcgc agttctttcg ccactatcag atgccgaacc cggaacgggc cccgcc
//
LOCUS       DASWZT010000265         5353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_55325, whole genome shotgun sequence.
ACCESSION   DASWZT010000265 DASWZT010000000
VERSION     DASWZT010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55325"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1391
                     /locus_tag="VGE92_07115"
     CDS             <1..1391
                     /locus_tag="VGE92_07115"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEY1313628.1"
                     /translation="TPGADWYWNFWRRAEAERGLDAVEDLLMPGTFTADLSPAQPVYL
                     VATAEAAAPAPGAAVLNSLKAASQRLTASLPKSAPAWVRTLAQASDQFIVRRGAARDA
                     FSVIAGYPWFTDWGRDTMISLPGLATSLRRYDVAAGILQTYAGFVDRGMLPNCFPDAG
                     ETPQYNTADATLWMFQALDDYLNAQSDPELVQRLFPILMRIIRAHASGTRYGIRVDPV
                     DGLLYAGEPGTQLTWMDAKHGDRVFTPRIGKPVEINALWLNALDVAVRLATSLRNISE
                     KSYCQSLLTHASSGFGRFWNEQRACLYDVLDTDHGGSADARIRPNQILAVSLPYCVLS
                     TTQRRAVVECCGRELLTSRGLRSLSPKDPGYIGVYTGDAGRRDAAYHMGTVWAWLLGP
                     FALAHYRVHGDARIAQSFLEPIAQHLDSVCLGTVSEIFDGDAPHSARGCFAQAWSVAE
                     ILRTWIFLERKI"
     gene            1388..4012
                     /locus_tag="VGE92_07120"
     CDS             1388..4012
                     /locus_tag="VGE92_07120"
                     /inference="COORDINATES: protein motif:HMM:NF015177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313629.1"
                     /translation="MNTERERLLANTPGPEGWQHWGPYLSERQWGTVREDYSSGGTAW
                     EYFPHDHARSRAYRWGEDGIAGISDADQLVCLSLAMWNGSDPILKERLFGLTNAQGNH
                     GEDVKEHYFYLDATPTHSYLKMLYKYPQRAFPYADLIAENARRGTELPEYELLDTGVF
                     AEDRYFDVFVEYAQERPGDILMKITAWNRAGAPAVLHLIPQLVLRNTWTWEPGSVKPK
                     LHAVGDGAVAIEPLDLGMTRLYFDGEADLLFTENESNAPRLWNSDGRGYFKDGFHERI
                     VGGNSDRVNPARCGTKAGVWFQGAIAAGGKREYRLRLSRQAIDMPFEGFDSCLTARNT
                     EADEFYSDLQKNMAADERVVQRQAFAGLIWSKQYYCFDIPRWLRGDPGQPPPPESRLH
                     GRDIEWRNLNNADIVSMPDKWEYPWYASWALAFHCIPLALIDAEFAKHQLVLLTREWY
                     MHPNGQIPAYEWAFSDVNPPVHAWAAWRVYQIDRKQNGGNGDLEFLERVFHKLMLNFT
                     WWVNRKDNLGRNIFQGGFLGLDNIGVFDRSAQLPTGGYINQSDGTSWMAMYSLNLMRI
                     ALELAEHNHVYEDIASKFFEHFLGIAEAMSSMLSADVGLWDEQDQFFYDVLNLPDNEK
                     VRLRIRSMVGLIPLFAVETLEPEMLERLPGFRKRLEWFLNHRKDLAGLVSHWESPGRG
                     QRRLLSLLRGHRMKSLLRRMLDESEFLSDYGVRALSRAHLASPFVLKIGDVDQSVRYT
                     PAESDSRQFGGNSNWRGPIWFPLNFLIVESLQKFHHYYGNDFKVECPTGSGHFLTIEE
                     VATELSHRLSRIFLKDANGERAVLRHQPQLQRDPNYKDCIPFYEYFHGDTGRGVGASH
                     QSGWTSLIAKLLMPRAHD"
     gene            4012..4788
                     /locus_tag="VGE92_07125"
     CDS             4012..4788
                     /locus_tag="VGE92_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEY1313630.1"
                     /translation="MPTASAARRALRAHWPEYLIEAWALATFMVSASIVTTELEAPGG
                     LLRGAIGNYRVRLALIGLAMGSTAVALIYSPWGKRSGAHMNPCVTLAFFSLGRISAID
                     SAFYIIAQFAGGLLGVLAAWALLGSAFAQPPVSFIVTLPGTAGTAAAFLAEAGISLML
                     MLAILEVSNSRRWSQYSGLVAGLLITCYVAFESPLSGMSMNPARTLASALPARNCVGL
                     WIYFSAPCLGMWAAARIFDASTPWAQRSHHSAKIVHTPES"
     gene            complement(4799..>5353)
                     /locus_tag="VGE92_07130"
     CDS             complement(4799..>5353)
                     /locus_tag="VGE92_07130"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY1313631.1"
                     /translation="LQRNPTMKRLVTLALGCAVFAAPLYAALKTGVQAPDFTTEATLA
                     GKPFKFSLASALKSGPVVLYFYPAAFTPGCTVEAHEFAEATDKFKALGATVIGVSHDP
                     IDKLNKFSVSECRNKFAVASDADQSITKAYDAVLAAKPEYANRTSYVIAPTGKIIYEY
                     TALDPDKHVGNTMAAVEQWKKEHP"
BASE COUNT          959 a         1646 c         1670 g         1078 t
ORIGIN      
        1 tcactcccgg agcggattgg tattggaact tctggcggcg agccgaagca gagcgcggtc
       61 tggacgcggt ggaggatctg ttgatgccgg gcactttcac cgcggatttg agcccggcgc
      121 aaccggtcta tctggtggct accgccgaag cggcggcgcc cgcgcccggc gcagcggtcc
      181 tcaattcttt gaaagcggcc tcgcaacgcc tgaccgcgtc gctgccgaaa agcgcacctg
      241 cgtgggttcg cacgctcgca caggcttccg accaattcat cgtgcgccgt ggtgcggcac
      301 gcgacgcctt cagtgtcatc gcggggtacc cctggttcac cgactggggg cgcgacacca
      361 tgatctcgct gccgggcctc gcgacctcgc tgcggcgcta cgacgttgcg gccggcatat
      421 tgcagaccta cgcgggcttc gtcgatcgcg gcatgctgcc caactgcttt ccggacgccg
      481 gcgagacgcc ccagtacaac acggcggatg ccacgctctg gatgttccag gcactcgacg
      541 attacttgaa cgcacagagc gatcccgaac tcgtgcaaag attgttccca attttgatga
      601 ggatcatccg tgcgcacgca agcgggacgc gctacggaat ccgcgtggat cccgtcgacg
      661 ggcttctgta tgcgggcgaa cctggcacgc agctgacctg gatggacgcc aagcacgggg
      721 atcgggtatt cactccccgc atcggcaagc ccgtggaaat caacgcgctc tggttgaatg
      781 cgttggatgt cgccgtgcgg ctggcgacga gcctcaggaa catcagcgag aagagctact
      841 gtcagtccct gctcacgcac gcgagttcgg gtttcggccg cttctggaat gaacagcgcg
      901 cctgcctgta cgacgtattg gataccgacc acggcggcag cgccgatgcg cgcattcggc
      961 ccaatcaaat cttggccgtc tccctgccct actgcgtcct gagcacgacg cagcggcgag
     1021 ccgtggtcga gtgctgcggc cgcgaattgc tcaccagccg cggcctgcgc agcctgagcc
     1081 cgaaggaccc gggctatatc ggcgtgtata cgggcgatgc cggtcggcgc gatgccgcct
     1141 accacatggg cacggtctgg gcctggttgc tcggcccatt tgccctcgcc cactatcggg
     1201 tgcacggcga tgcgcggatc gcgcaatcgt ttctcgagcc catcgcgcag cacctggaca
     1261 gcgtctgctt gggaaccgtc agcgagatat tcgatggtga cgcaccgcac agtgcgcgcg
     1321 gttgtttcgc ccaagcgtgg agcgtcgctg agatactgcg cacttggatt ttcctcgagc
     1381 gcaaaatatg aacaccgagc gcgagcgcct gctggcaaac acgcccggcc ccgaaggctg
     1441 gcaacactgg ggaccttact taagcgaacg ccagtggggc acggtgcgcg aggactacag
     1501 ctccggcggc acggcgtggg aatactttcc gcatgatcac gcgcgcagcc gcgcgtaccg
     1561 ctggggcgaa gacgggattg caggaatttc cgacgccgac caattggtgt gcctgtcgct
     1621 ggcgatgtgg aacggctccg acccgattct aaaggaacga ttgttcggcc tgaccaatgc
     1681 ccaaggcaat cacggtgagg acgtcaaaga acattatttt tatttggacg cgacgccgac
     1741 gcactcctac ttgaagatgc tctacaagta cccgcaacgc gcctttccgt acgcggactt
     1801 gattgcggag aacgcgcggc gcggcacgga gcttccggag tacgagcttt tggacaccgg
     1861 cgtgtttgcc gaggatcgct acttcgatgt ctttgtcgag tacgcgcaag agcggccggg
     1921 cgatattctc atgaagatta cggcctggaa tcgcgccggc gcacccgccg ttctgcatct
     1981 gattccgcag ctggtgctgc gcaacacatg gacctgggag cctggctcgg tgaagccgaa
     2041 gttgcacgcg gtcggtgacg gcgccgtcgc catcgagccc ctcgatctgg ggatgactcg
     2101 actctacttc gacggcgagg ccgatctctt gtttaccgag aacgagtcca atgcgcctcg
     2161 cctctggaat tccgacggcc gcggatattt caaggatggc ttccacgagc gcatcgttgg
     2221 cggaaattcc gatcgcgtca acccggcgcg atgcggcacc aaagccgggg tttggttcca
     2281 aggcgccatt gccgccggcg gcaaacgcga gtaccgcctg cggctgtcac gccaagccat
     2341 cgacatgcct ttcgaaggat tcgacagctg cttgacggcg cgcaacactg aggccgatga
     2401 gttctattcc gacctgcaga agaacatggc ggccgacgag cgcgtggtgc agcggcaggc
     2461 atttgccgga ctgatttgga gcaaacagta ctactgcttc gacattccgc gctggcttag
     2521 gggcgatccc ggccagccgc cgccgcccga gtcgcgcctg cacggccgcg acatcgagtg
     2581 gcgcaatctc aacaacgccg acatcgtgtc catgccggac aaatgggagt acccgtggta
     2641 cgcatcgtgg gccttggcct tccattgcat tcctctcgcc ttgatcgacg ccgagttcgc
     2701 caagcatcag ctggtgctgc ttacgcgcga atggtacatg caccccaacg gccaaattcc
     2761 ggcgtacgag tgggcattca gcgatgtaaa cccgccggtg catgcctggg ccgcgtggcg
     2821 tgtgtatcag atcgaccgca aacagaacgg cggaaacggc gacctggagt ttctcgagcg
     2881 cgtgtttcac aagctcatgc tgaatttcac ctggtgggtc aatcgcaagg acaacttagg
     2941 ccgtaatatt tttcagggcg gcttcttggg actcgacaac atcggcgtgt tcgatcgcag
     3001 cgcgcagctg ccgaccggcg gctatatcaa tcagtccgac ggcaccagct ggatggcgat
     3061 gtattcgctc aatctcatgc gcattgcact cgaactcgcc gagcacaatc acgtgtacga
     3121 ggatatcgcc agcaaattct tcgagcattt cctcggcatc gccgaggcca tgagcagcat
     3181 gctgagcgca gatgtcggtc tttgggacga acaagaccag ttcttctacg acgtattgaa
     3241 cctgccggac aacgaaaaag tgcggctgcg gattcgttcc atggtgggat tgattccttt
     3301 gttcgcggtc gaaaccttgg agccggagat gctggaacgg ctgccgggat ttcgcaaacg
     3361 cctggagtgg ttcttgaacc accgcaagga tctcgcgggc ctggtctcgc attgggagtc
     3421 gccggggcgc ggccagcgcc ggctgttgtc gctactgcgc ggtcatcgaa tgaagagtct
     3481 gctgcggcgg atgctggacg agtccgaatt cctttccgac tatggcgttc gggcgctgtc
     3541 gcgcgcgcat cttgcgagcc ccttcgtgct gaaaataggc gacgtcgatc agtcggtgcg
     3601 ctacacgccg gccgaatccg attcgcggca gttcggcggc aattccaact ggcgcgggcc
     3661 gatctggttt cccctgaatt tcttgatcgt cgaatcgctg cagaagtttc atcactacta
     3721 cggcaacgat ttcaaggtcg aatgccctac cggctccggc cactttctca ccatcgagga
     3781 agtggcgacc gagctgtcgc atcgcttgtc gcgcatattc ctcaaggatg ccaacggcga
     3841 gcgggcggtg ctccgtcatc agccgcagct gcagagagat ccgaactaca aggactgcat
     3901 cccgttctac gagtactttc acggcgacac cggccgcggc gtcggcgcat cgcaccagtc
     3961 ggggtggacc tcgctgatcg cgaagctgct catgccgaga gcgcatgact agtgccgacg
     4021 gctagtgcgg cgcgccgtgc attgcgcgcg cattggccgg agtacctgat cgaggcctgg
     4081 gcgctcgcca cgttcatggt gtccgcctcg atcgtcacca cggagctcga ggcgcccggc
     4141 gggttgctgc gcggcgcaat cggcaattat cgggttcgct tggccctgat cgggctcgcc
     4201 atgggttcga ctgccgtggc actcatctat tcgccctggg gcaagcgttc cggcgcgcat
     4261 atgaatccgt gcgttaccct cgcattcttc agcctcggcc gcatttcggc catcgattcg
     4321 gcgttctaca tcatcgcgca gtttgccgga ggattgctcg gcgtgctcgc ggcatgggcg
     4381 ctgctaggct cggccttcgc ccagccgccg gtgtcgttca tcgtcacgct gccgggcacg
     4441 gccgggacgg ctgccgcttt cctcgccgag gcgggcattt cgctgatgtt gatgctggcc
     4501 atcctcgagg tatccaacag ccggcgctgg tcgcagtatt cgggcttggt cgcgggatta
     4561 ttgattacct gctacgttgc tttcgaatcg ccgctgtcgg ggatgagcat gaacccggcg
     4621 cgcacgctgg cctcggcgtt gccggccagg aattgcgtcg gcctgtggat ttacttttcg
     4681 gcgccctgcc tcggcatgtg ggcggcggcg cggatctttg atgcctcgac gccgtgggcc
     4741 cagcgttcgc accatagcgc caagattgtt cacactccgg aaagctagga gcctcgggct
     4801 acgggtgctc tttcttccat tgctccaccg ccgccatggt gtttccgaca tgtttgtcgg
     4861 ggtcgagcgc ggtgtattca tagatgatct tgccggtcgg cgcgatcacg taggaagttc
     4921 gattggcgta ttctggcttc gccgcaagta ccgcgtcata cgccttggtt atgctctgat
     4981 ccgcatccga ggccaccgcg aacttgttgc ggcactcgct cacggaaaac ttattgagct
     5041 tgtcgatcgg atcgtgcgac acgccgatta cggtcgcccc taaagccttg aatttgtcgg
     5101 tggcctcggc gaactcgtgg gcctccaccg tgcagccggg cgtgaacgcg gctggataaa
     5161 aatacaaaac gacgggaccg cttttcagcg cgctggcgag agagaacttg aacggcttgc
     5221 cggcgagcgt agcctcggtc gtgaaatcgg gcgcttggac accggtcttc aaagccgcgt
     5281 acaggggagc ggcaaatacc gcacaaccca gggcgagagt cactaatcgc ttcatggtag
     5341 ggttcctttg cag
//
LOCUS       DASWZT010000266         1909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_50301, whole genome shotgun sequence.
ACCESSION   DASWZT010000266 DASWZT010000000
VERSION     DASWZT010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50301"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VGE92_07135"
     CDS             <1..888
                     /locus_tag="VGE92_07135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY1313632.1"
                     /translation="VVSIIVPVFDRLAYLRAAIDSILEQSFQDWELIIGDDGSGPETL
                     DYLRTLEHVARVQVLQLAHSGNPAVVRNAALRRATGTYVAFQDSDDVWMPDKLLIQVA
                     ALRSRTACHWSYTGFTLVDDCGQPLTGPQAAPCPAVSGRIFERLLREQALVVTPSVVV
                     RRELINELGGYNESLQVCEDYELWLRLALRSNVDFIDQPLVLIRRHSQHSFDDLTCLE
                     NLRRTLEIVQHSAAAPHLHGVLDERRAKISANMARGYALRRHRVRALATLLSSARYSW
                     RYPYWWRGALLAGARAISP"
     gene            885..>1909
                     /locus_tag="VGE92_07140"
     CDS             885..>1909
                     /locus_tag="VGE92_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY1313633.1"
                     /translation="MSTLLAICKLLDRRQRGQLIAMQIVSVLMALSTVGGIAAVLPFF
                     SVLAEPRAIVAHPALRSLYEYLHLGGESRFVIALGGGFAGAIVLSNLINLVGSLAIDR
                     FAFRVGDALHSALFDEYLHRDYEFHSSSSSATLTSNVLYETGRVAGGILRHGLILVSG
                     LATVLCILASIVLLNPLVAMLAIAGLGVSYGAIYAAVRGRLRRNGEIETEEFAERTRI
                     VSESFGAIRELIVLRAQPYFVAKLARCCQSISATIVSNLAIAQTPRHVLECATACVLV
                     GVALYSRINGQSVGPLIAELSFIGLAVYRLLPALQQVFQAIVRIRSDSPAFERIAADL
                     QRARSRQT"
BASE COUNT          313 a          594 c          595 g          407 t
ORIGIN      
        1 gttgtctcca tcatcgtgcc ggtattcgat cgcctggcgt acctgcgcgc ggccatcgat
       61 tcgatactcg agcagagttt tcaggactgg gaactgatca tcggcgacga cggctcgggt
      121 ccggagacgc tggattatct gcgcacgctc gagcacgtag cgcgagtcca ggtgctgcag
      181 cttgcgcaca gtggcaatcc cgcagtggtg cgcaacgccg ccttgcgccg cgccaccggg
      241 acatatgtgg cgttccagga ttccgacgac gtgtggatgc ccgacaaatt gctgatacaa
      301 gtcgcggcgc tgcgctctcg taccgcttgc cactggagct atacgggttt cacgctcgtc
      361 gatgactgcg gccagccgtt gaccgggccg caggccgcac cgtgccccgc agtcagcggc
      421 cggatcttcg agcggttgct gcgcgaacaa gccttggtcg tgactccgag cgtcgtggtg
      481 cgccgcgagt tgatcaacga gcttggcgga tacaatgagt cgctgcaggt gtgcgaagac
      541 tatgagttat ggctgcggct ggcgttgcga agcaacgtcg atttcatcga ccagccgttg
      601 gttttgatta ggcggcacag ccagcactcg ttcgacgacc tcacttgcct ggagaacttg
      661 cggcgcaccc tggaaatcgt ccagcattcg gccgcggcgc cgcacctgca tggcgtcctt
      721 gatgagcggc gggcaaagat ctccgccaac atggccagag gctatgctct gcgcaggcat
      781 cgagtgcgcg ccctcgccac cctattatcg agcgcccgct actcgtggcg ttacccatac
      841 tggtggcgag gcgcgctgct agcgggcgcg cgcgcgatct caccgtgagc actctgcttg
      901 ccatctgcaa gctgctcgat cgccggcaac gcggtcaatt gatagccatg caaattgtct
      961 cggtgttgat ggcgctgtcg acggtgggcg gcattgcggc ggtgctgccg ttttttagcg
     1021 ttctcgccga gccgcgcgca atcgtcgctc acccagctct gcgctctttg tacgaatacc
     1081 tccaccttgg cggagaaagt cgctttgtga tcgccctcgg cgggggcttc gcaggcgcca
     1141 tcgtgctgtc gaacctcatc aacctggtcg gctcgctcgc catcgaccgc ttcgcattcc
     1201 gcgtcggcga cgccctgcac agcgctttgt tcgacgaata tttgcatcgc gattacgaat
     1261 ttcattcaag ctcgagcagc gcgacgctca cgagcaacgt tctctacgag acgggccgcg
     1321 tcgcgggcgg tattctgcgg cacggcttga ttctggtgag cggcttggcc acggtgctgt
     1381 gcatcctggc gtcgatcgtg ctgctgaacc cgctggtggc catgcttgcg attgcaggac
     1441 tcggcgtcag ctatggggcg atctacgcgg ccgtgcgtgg tcggttgcgg cgcaacggcg
     1501 agatcgaaac cgaagaattc gcagaacgca cgcggatcgt cagtgaaagc ttcggtgcca
     1561 ttcgggaact catcgtgctg cgagcgcagc cttatttcgt ggcgaagctc gcgcggtgct
     1621 gccaatcgat ctcggccacc attgtcagca atctcgccat tgcgcagacc ccgcggcatg
     1681 ttctggaatg tgcgaccgcc tgcgttttgg tcggcgtcgc gctttactca cgcatcaacg
     1741 gccagtcggt cggcccattg atcgccgaac tgagcttcat cggtctcgcc gtctaccgtc
     1801 tgttgccggc cctgcagcag gtattccagg caattgttag aattcgatcg gactcgccgg
     1861 cctttgagcg tatcgcggcc gacttgcaac gagcgcggtc acgccagac
//
LOCUS       DASWZT010000267        12323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_35852, whole genome shotgun sequence.
ACCESSION   DASWZT010000267 DASWZT010000000
VERSION     DASWZT010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12323
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35852"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1298
                     /locus_tag="VGE92_07145"
     CDS             <1..1298
                     /locus_tag="VGE92_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005262829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1313634.1"
                     /translation="FVPRLGAVAMAQADARSLMTGRITDAYTNITTVKLFSHTQREAG
                     YVQGAMAEFLQTVYRQMRMVTGFEIVNHGLSMGLIASTAGVTLWLWTQNRVGVGAVAA
                     TTAMALRLNGISHWVMWEMASLFEQIGTVQDGITTLARSPTVLDAADAQALRVTRGDI
                     RFEQVSFAYGAVSNPSRPVIDNLNLHIRPGEKIGLVGRSGAGKSTIVNLLLRFYDISH
                     GRVLIDGQDIAHATQDSLRAQIGMVTQDTSLLHRSVSDNICYGRPDASEADMLAAAKR
                     AEAHDFIVALSDAKGRTGYQAHVGERGVKLSGGQRQRIAIARVMLKDAPILLLDEATS
                     ALDSEVEAAIQQSLYRLMEGKTVVAIAHRLSTIAAMDRLIVLDKGRIVEEGNHYSLLA
                     EGGLYARLWAHQSGGFLGEDAEENGLPPRQMSAATLSPS"
     gene            complement(1265..3208)
                     /locus_tag="VGE92_07150"
     CDS             complement(1265..3208)
                     /locus_tag="VGE92_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1313635.1"
                     /translation="MTHLFQPLRIRGCTLKNRIMSTGHDTTLPVDGTVNAALVAYQEA
                     RARGGVGLIVLQVSGVHETARYTNHVLMATDDRSIEGYRNVAQAVHRHGTVLFAQLFH
                     PGREMAEADGGLLNVAYAPSSVPNERFHVMPRALQKPMIDSIVRGYGAAARRMQSAGL
                     DGVEIVASHGYLPAQFLNPRVNVRSDAYGGDIQGRLRFLREVIREIRAQASADFVVGL
                     RISGSEVDEQGLTQDETLEAVAQLGDSIDYVHVTLGTSASLGGAIHIAPPMAFKSAYV
                     VPYAARIKRQSRIPVFVTGRINQPQEADAVIAANHADVCGMTRALICDPEMPNKTLRG
                     ALDDIRACIACNQACIGHFHKGYPISCIQNPVSGRELVFGTLAPAARAKRVMVIGGGP
                     AGMKAAAIAAERGHHTSLYEAERRLGGQALLAQLLPGRAEFGGIITNLKREMELAGVH
                     VVTGARIDRAAVSEAAPDVVVIATGAVPYRPEFPQEGALQIVDAWQVLRGQTVVGQSV
                     VVVDWRADWIGIGIAEHLAQQGRSVRLAVSGIAAGETLPFYVRDQAAGSLHKLGIKVL
                     TYMRLYGSDADSVYLQHQSSGEAVVIDKVDTLVLCTGHSPVEELSEALQGLDMQVHVI
                     GDAAAPRTAEEAVYEGLKVAADI"
     gene            3478..3789
                     /locus_tag="VGE92_07155"
     CDS             3478..3789
                     /locus_tag="VGE92_07155"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HEY1313636.1"
                     /translation="MKTSRKALLVGLMMAAGAVAAPSIASAGVAIDIDVAPPPVRVEA
                     VPEPRVGFAWAPGFWEWQGGAHVWVPGHYIRERRGYHWVADRWDQRGPHWHHERGHWE
                     R"
     gene            3868..4374
                     /locus_tag="VGE92_07160"
     CDS             3868..4374
                     /locus_tag="VGE92_07160"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY1313637.1"
                     /translation="MSTPAALRIGLIADTHGLLRPEARAFLAGCDYIVHGGDIGRAEI
                     LDELGAMAPLIAIRGNNDTAPWAAALPQTELVRMGGIFVYVIHNLAELAIEPAARGVR
                     AIISGHSHIPRVAEKDGVLYVNPGSCGPRRFKLPICIGELLLTGSRLSARVVDLIGGV
                     DVATTTVM"
     gene            complement(4585..5316)
                     /gene="aqpZ"
                     /locus_tag="VGE92_07165"
     CDS             complement(4585..5316)
                     /gene="aqpZ"
                     /locus_tag="VGE92_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005418268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HEY1313638.1"
                     /translation="MPLSKKLAAEFIGTFWLVLGGCGSAVLAAAFPHVGIGLLGVSLA
                     FGLTVVTMAFAIGHVSGCHLNPAVTLGLVVGGRHPAKEMFPYWVVQVLGGIAAAWVLL
                     FIAAGNGTDPLAGGLAANGFADHSPGNYSMVSGFVCEVVMTAIFLFVIMGATDKKAPV
                     GFAPLAIGLTLTLIHLISIPVTNTSVNPARSTGPAVFVQGWALEQLWLFWAAPLLGGA
                     LGAGIYRFVTPGWIQGPGDPGGPGT"
     gene            5491..6954
                     /locus_tag="VGE92_07170"
     CDS             5491..6954
                     /locus_tag="VGE92_07170"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY1313639.1"
                     /translation="MMLSILVCHSHFLRLDHKQVLRAKPYPPLATLQVAAMLRQAGHR
                     VSFFDAMLAEGIDEYHSVLQAQHPQLLLFYEDNFNFLSKMCLGAMRRAACEMIVSARR
                     AGARVIAAGPDVSDAAGPYLKAGADLALVGEGLSALFEILSRLDANPETDRADLIRGV
                     GGVVAFAAGQALTKSASRASPILAEVGLAAWDLVDMQRYRSVWLRAHGYFSLNMAASR
                     GCPFRCAWCAKPIWGNQYLQRNAAEVAGEMAHLKRSFHPDHIWFADDIFGFRVDWVAQ
                     FAAAVQAADAQIPFTIQMRADLVSERMAEALRDAGCREVWIGAESGSQRVLDAMNKGT
                     TVAEIRIARERLKSVGIRVGFFIQLGYMHEQLEDLQATRDLLHAARPDEIGVSVSYPL
                     PGTKFYELVKAQLRDKTHWQESNDLEMMFQGTYASDFYRAVRNLLHDQIALQTAVDAP
                     DGESHGRARRALDQRWQDLMAQELQYRSPLDRSAAAG"
     gene            6951..8174
                     /locus_tag="VGE92_07175"
     CDS             6951..8174
                     /locus_tag="VGE92_07175"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY1313640.1"
                     /translation="MKTRLPGPKTLQRMLRTLTEALARELARPSPSAPLWTDIEWRLA
                     HAVCAMHGVSPLLARCLPWSGPGGWRQFLEEQRLHTKNRHARITQFLREIDQRMGEAG
                     VAAVALKGVALHALGVYRAGDRPMADIDLLVRPADTQRSARALESMGLRECYRTWKER
                     VFTPVDGHPPGALGEHSNNNIKLELHERIGERLPWQIVDISEHIFPRDSRPGLNAYPS
                     TASLMMHLLLHAAGSMAMQGLRLMHVHDLAQLSLRMTHRDWDELLAYRPCAQPLWWAL
                     PPLKLTARYYSSQVPPRVLEALAEQCPYLLARVAGRKTLSDVSYSYPWVDALPGIEWS
                     QSLREVLQYAHSRVRPTATQIALREQFAHTQGGGLQSPWHRLSQRKRMVRWITSNPTR
                     PLTMHAVAAALAPPP"
     gene            8183..9025
                     /locus_tag="VGE92_07180"
     CDS             8183..9025
                     /locus_tag="VGE92_07180"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY1313641.1"
                     /translation="MAPAAAPAAGLLGDTPARNYSRKLAQFNAFAAPELKRLIAEIGL
                     SPGMRVLDAGCGTGEALPWLLEAAPSGHVVGIDLSAAHVSEARRHAGAQIEILQGDLA
                     QAPLAPASIDFIWCVNTINHLHDPLQGVARLAALLRPNGRIALGQSSLLPDMYFAWDS
                     RLERVTNEAVRHYYRERYALQERDLAAVRAIVGVLRAAGLKSVGTKTLLIERISPLDT
                     ATECYLRETLFRDTWGAGLRPYLSHEDFTELGCLCDPEHPKFALRRPDFHFMQTFTVA
                     MGTR"
     gene            complement(9022..10119)
                     /locus_tag="VGE92_07185"
     CDS             complement(9022..10119)
                     /locus_tag="VGE92_07185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY1313642.1"
                     /translation="MRIALVVPGGVDRSGEYRVIPALLALIARLSAHHDVQVIALRQE
                     LRAGAWNLGGARVHNIGVRPTALRALAAIYKLHRTRSFDLMHAIWSGPCGLVAVIAGR
                     ALGIPSLVHIAGGELVSLPDIAYGGAQTRLGRWREGWALRHASAITAASAPVIASLNA
                     CGFAAQRIPLGVDLSLWPPRQPVARRNDRPAQLIHVASLNRVKDQPTLLRALAVLKNS
                     GMRFEMHIIGEDTLLGEIQVLTRQLKLSDSVEFHGFLTQRQLRPLMELADVMVLSSRH
                     ETGPLVALEAAVAGVPTVGTRVGHIAEWAPFAAKSASVGDSAGLAAAVRQLLENEDLR
                     MSVAREALRRATREDADHTATRFSALYEKLL"
     gene            complement(10120..11106)
                     /locus_tag="VGE92_07190"
     CDS             complement(10120..11106)
                     /locus_tag="VGE92_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313643.1"
                     /translation="MSDPFGERSAGFTQRMRLLGAKFRFDSNSEELLGLVNCAYQGLP
                     RHRLSGYDGELRVSLLLTGGKQRLGRGEPPPLKMLSGAALLAGATPEADFAVISPRAH
                     SALVVVSPRMLRFPYHTRYELIEFAVFTLAARAQRLVSLHAACVGRHGRALLLIGPSG
                     SGKTTVALHCLLDGFDFLSEDSAFVSPDTMLATGVSNYVHIRADSLRWLGRSRVAAVI
                     RQSPVIRRRSGVKKFEVNLRRGGFRLARSPLKIVGTVFVLPHSAGDRPVLQAISKASL
                     LANLADTQGYAAGQPPWADFRRNMSRLDCFELRRGSHPREAVQALRSVLDAR"
     gene            11179..11997
                     /locus_tag="VGE92_07195"
     CDS             11179..11997
                     /locus_tag="VGE92_07195"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY1313644.1"
                     /translation="MTTLSGPQLHDTRDAFDSVAADYDGPRGNNDQIQEMRRDMWRSL
                     DAAFPGPSRLIDLGCGTGLDAVRMAKRGHVVTAMDWSGAMVQRTRERAQSEHVQDWIE
                     PVALGAHELRHLNGAGRYDGVYSNLGPLNCVPDLGDVSQECARLLRPDGVLVFTVIGR
                     YCPWEIAHYLRLRRWARATVRFHRQVVPISMNEHTIWARYFGPREFYRAFRQNFRLEH
                     HRGLCLFSPPPYLTWVREHHPVWHERLQRLDRRVSHWPLLRAMGDHFLIVMKKR"
     gene            11940..>12323
                     /locus_tag="VGE92_07200"
     CDS             11940..>12323
                     /locus_tag="VGE92_07200"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEY1313645.1"
                     /translation="MAVAACNGGPFSDRDEEAVTTAVRDRVTIFDGLQGVAAIAVVLL
                     HGCSIFNLGWLPAHAYLAVDFFFLLSGYVIARAFDERLRQGWLFGFLQRRLIRLYPLI
                     VVGSLVGFIEMIIHGVLTHSVSISQA"
BASE COUNT         2251 a         3825 c         3946 g         2301 t
ORIGIN      
        1 acttcgtacc gcggctcggc gcagtcgcca tggcccaggc cgatgcgcgt tcgctgatga
       61 ccggccggat caccgatgcc tacaccaaca tcacgaccgt caagctgttc tcgcatacgc
      121 agcgcgaagc cggctacgta cagggcgcca tggcggagtt cctgcagacg gtgtatcggc
      181 aaatgcggat ggtaaccggg ttcgagattg tcaaccacgg cctcagcatg ggactgattg
      241 cgagcacggc cggcgtcacg ctgtggcttt ggacgcaaaa ccgggtgggc gtcggcgcgg
      301 tcgccgcgac gaccgccatg gcgctgcggc tcaacggaat ttcgcactgg gtcatgtggg
      361 aaatggcatc gttgttcgaa caaatcggta cggtgcagga cggcatcacc acgctggcac
      421 gctcgcccac ggtgctcgac gctgcggacg ctcaggcgct gcgcgtgacg cgcggcgaca
      481 ttcgcttcga acaggtgagt ttcgcgtatg gcgcggtctc gaacccgtcc cgaccggtca
      541 tcgacaatct gaatctgcac atccgcccgg gcgagaaaat cggtctggta gggcgctccg
      601 gcgccggcaa gagcaccatc gtcaatctgt tgctgcgctt ctacgacatt tcccacgggc
      661 gggtgttgat cgacggtcag gacattgcgc acgcgacgca ggatagtcta cgcgcacaaa
      721 tcggtatggt gacccaggac acctcgctcc tgcaccgctc ggtgagcgac aacatttgtt
      781 atggccggcc ggatgccagc gaggccgaca tgctggctgc ggccaagcgc gccgaggcac
      841 acgatttcat cgtggcgctg agcgatgcca agggacgcac cggctaccag gcgcatgtgg
      901 gcgagcgtgg cgtgaagctc agcggcggac agcgccagcg cattgccatc gcgcgcgtga
      961 tgctgaagga cgccccgata ctgctgctcg atgaagccac cagcgcgctc gacagcgaag
     1021 tcgaagccgc cattcagcag agtctgtatc gcttgatgga aggcaagacc gtggtcgcga
     1081 tcgcgcatcg gctctcgacc atcgctgcca tggaccggtt gatcgtgctc gacaaaggcc
     1141 gcatcgtcga ggagggcaat cactattcct tgctcgccga aggcggattg tacgcgcggc
     1201 tgtgggcgca tcagagcggc ggattcttgg gcgaggacgc cgaggagaac gggctgccgc
     1261 cgcgtcaaat gtcggcggct accttgagtc cttcgtagac ggcctcctcg gccgtgcgcg
     1321 gtgccgccgc gtcgccgatg acgtggacct gcatgtcgag accttgcaga gcctcggaca
     1381 gttcctcgac cggactgtgg ccggtacaca gcaccaacgt gtcgaccttg tcgatgacca
     1441 ccgcttcgcc gctgctctga tgctgcaaat agacgctgtc cgcgtcgctg ccgtagaggc
     1501 gcatgtacgt gaggaccttg attcccaatt tgtgcagcga acccgcggcc tggtcgcgta
     1561 cgtaaaacgg cagggtttcg ccggcggcaa tgccactgac cgccaagcgc acactgcggc
     1621 cctgttgtgc cagatgttcg gcgatcccga tgcctatcca atccgcgcgc caatccacga
     1681 ccaccaccga ttgaccgacc acggtctgac cacgcagcac ttgccacgcg tcgacgattt
     1741 gcagtgcgcc ttcctgcggg aattcggggc ggtagggcac ggctccggta gcgatcacga
     1801 ccacgtccgg agcggcttcg ctcactgccg cgcggtctat gcgtgcaccg gtaacgacgt
     1861 gcactcctgc taattccatt tcgcgcttga gattggtgat gatgccgccg aactccgcgc
     1921 ggcccggcag caattgcgcg agcaacgcct ggccgcccaa gcgccgctcg gcctcgtaga
     1981 ggctggtgtg atgaccgcgt tcggctgcga ttgccgccgc cttcatgccg gccgggccgc
     2041 cgcctatgac catcacccgt ttggctcgcg cggcaggggc cagcgttccg aagacgagct
     2101 cacgtccgct caccggattc tggatgcagg aaattggata gcccttgtga aagtggccga
     2161 tgcacgcttg attgcaggcg atgcaggcgc ggatgtcatc gagcgcgccc cgcagggttt
     2221 tgttcggcat ctcaggatcg caaatcaggg cgcgtgtcat gccgcacacg tccgcatggt
     2281 tggcggcaat cacggcatcc gcctcttgcg gctgattgat gcggccggtc acgaacaccg
     2341 gtatgcgcga ttggcgcttg atacgcgccg catacggcac gacgtaagcg ctcttgaacg
     2401 ccatgggcgg tgcgatatga atggccccgc ccaaactcgc ggaggtgccg agcgtgacgt
     2461 gcacgtagtc gatgctgtcg ccgagctgcg caacggcttc gagagtctca tcctgcgtca
     2521 aaccttgctc atcgacctcg gaacctgaaa ttctcaggcc caccacgaaa tcggcgctcg
     2581 cctgggcgcg gatctcgcgg atcacttcac gcaagaagcg caggcggccc tgaatgtcgc
     2641 cgccgtacgc gtcgctgcgc acgttgacgc gggggttcaa gaactgcgcc ggcaaatagc
     2701 catggctggc gacgatttcc acgccgtcca atcccgcgct ctgcatgcgc cgggccgccg
     2761 cgccgtagcc gcgcacgatg gagtcgatca tcggcttctg caacgcgcgc ggcatgacat
     2821 gaaagcgctc gttcggcacg ctggaaggtg cgtaggccac gttcagcaat ccgccgtccg
     2881 cttcggccat ctcgcggccg ggatggaaca gctgggcaaa gagcacggta ccgtggcgat
     2941 gcaccgcttg cgcgacgttg cggtaaccct cgatcgagcg atcgtcggtc gccatcagca
     3001 cgtggttggt atagcgcgcc gtctcgtgga ccccagatac ctgcagcacg atcaagccca
     3061 cgccgccgcg ggcgcgcgcc tcctgatacg ccaccagggc cgcgttgacg gtgccgtcca
     3121 ccggcagggt cgtatcgtga cccgtcgaca tgatgcgatt cttgagcgtg cagccgcgaa
     3181 ttcgcagcgg ctggaaaaga tgcgtcaatg ccgtcatgca cgaatagtaa tatatccgcc
     3241 gtcaccatcg cgtggcgccg gccgatcatc cgcaggcagc agcagattac cgactcttaa
     3301 ggccgaagcg atcacaccgt tttctgcaga ttgatcaata gtttaggacc gctcaggggt
     3361 ctgccgaatg cggcgctgtc ctacagacac ccaatcgacg tcaactcacg ataaccctca
     3421 cgcgtttgag cactaggttt gttcgcattg ggttcgacac taactaagga gtccgcaatg
     3481 aaaacttctc gcaaggcgct gctggtgggc ctgatgatgg ccgccggtgc ggttgccgcg
     3541 cccagcatcg cgtcagccgg agtcgccatc gacatcgacg tcgcgccgcc gcccgtacgc
     3601 gtcgaggccg tcccagaacc gcgcgtcgga tttgcctggg cgccgggatt ttgggaatgg
     3661 cagggcggcg cccacgtgtg ggtgccggga cattacatcc gcgagcgccg cggctatcac
     3721 tgggtggccg accgttggga tcagcgcgga ccgcattggc atcatgagcg gggccactgg
     3781 gagcgataag cgcggttgag gtaagcttaa gcgggcgggt gggggtgttc ttccctcacc
     3841 cgcacatgcg ttaaagtaca tgctcccgtg agcacgcccg ctgcgctacg aattggactc
     3901 attgccgata cccacggcct cctgcggccc gaggcgcgcg cgttcctcgc cggttgcgat
     3961 tacatcgttc acggcggcga catcgggcgt gccgaaattc tcgatgaatt gggcgcaatg
     4021 gcgccgttga ttgcgatccg cggcaacaac gacaccgcgc cctgggcggc agccctcccg
     4081 cagaccgaat tggtgcgcat gggcggcatc ttcgtttacg tcattcacaa tctcgcggag
     4141 ctcgccatcg aacctgccgc gcgcggagtg cgcgcgatca tctcggggca ctcgcacata
     4201 ccgcgagtgg cggagaagga cggcgttttg tacgtcaatc caggaagctg cggcccgaga
     4261 cgtttcaaac ttccgatttg catcggggaa ctcctgctga ccggcagccg actgagcgca
     4321 cgtgtcgttg atttaatcgg aggcgtcgat gttgcaacta cgaccgtcat gtgagtgctg
     4381 cgattcgccg ctgccgccgg actccaccga ggcgcggatc tgctcgttcg agtgtacgtt
     4441 ctgcgcggtg tgtgcggaca cacggctgca cggccgctgc cccaactgcg gcggggaatt
     4501 gctcgcgcgg ccgaggcgcc cggcccaaga gttgatggca tacccggcat cgacccggcg
     4561 gatttacaat ccaggcggct gtccttaagt tccggggccg ccgggatccc ccgggccttg
     4621 aatccatccc ggagtgacga atcggtagat ccctgccccg agcgcgcccc ccaatagggg
     4681 tgcggcccag aagagccaca gttgctcgag tgcccaacct tgcacgaaca cggcaggccc
     4741 ggtgctgcgc gcgggattca cggacgtgtt ggtcacgggg atgctgatca gatggatcag
     4801 cgtgagcgtg aggccaatgg cgagcggcgc gaagccgacg ggcgcctttt tgtcggtggc
     4861 tcccatgatg acaaatagaa aaatcgcggt catcacgact tcacagacga aacccgacac
     4921 catgctgtag ttgccgggcg agtgatcggc aaacccgttg gcggcaagac cgccggcgag
     4981 aggatccgtg ccgttgcctg cggcaatgaa cagcaatacc caggctgcgg caatgccgcc
     5041 aagcacttga acgacccagt agggaaacat ttccttggcg ggatggcggc cgccgaccac
     5101 caatcccaag gtgacggcgg gattcaagtg gcaacccgac acatgaccga tggcaaaagc
     5161 catggtgacc accgtgaggc cgaaggccag cgacaccccg agcaggccga ttccgacgtg
     5221 cgggaaggct gcggccagca cggcgctgcc acagccgccg agcaccaacc aaaacgtacc
     5281 gatgaactcg gcggcgagtt tcttcgagag cggcatcgtt gactcctgtc ttttcttgtc
     5341 gttctgaccg ggccgcaaaa ataccagatt cgaggttaaa cctaaacttc cgatccaagc
     5401 actcactcgt tacatcgcgc tatgtcatac gcgcgagacg cggccgcact ggtgggcgcg
     5461 gcaaattggt gcgccgaatt tgctggagcg atgatgcttt caatcttggt ttgccattcg
     5521 catttccttc gcctcgatca caagcaggta ctgcgcgcga agccctatcc gccgctcgcc
     5581 accttgcagg tggccgcgat gctgcgtcag gccgggcatc gagtatcgtt cttcgatgcg
     5641 atgctggccg agggaatcga cgaatatcat tccgtgctgc aggcccagca tccgcagctg
     5701 ctgctcttct acgaagataa tttcaacttc ctgagcaaga tgtgcctggg cgcgatgcgg
     5761 cgcgccgcct gcgaaatgat cgtctcggcg cgccgtgccg gcgcccgggt cattgcggcc
     5821 gggcccgatg tcagcgacgc tgcggggccc tatctcaaag ccggcgccga tctggcgttg
     5881 gtcggcgagg gactatcggc gctgtttgag attctgtcgc ggctcgatgc caatcccgag
     5941 accgatcgcg cggatctcat ccgcggagtc ggcggggtgg tggcgtttgc cgccggtcag
     6001 gcattgacga agagtgcttc cagggcatcg ccgatcttgg ctgaagttgg tctagcggca
     6061 tgggatttgg ttgacatgca gcgttatcgc agcgtgtggc tacgagcgca cggttatttc
     6121 agcttgaaca tggccgcttc gcgcggctgc ccgttccgct gtgcttggtg cgcaaaaccc
     6181 atttggggca atcagtacct gcagcgaaat gccgcggagg tcgcgggtga aatggctcat
     6241 ctgaagcgat ccttccatcc tgaccacatt tggttcgcgg acgacatttt tggatttcgc
     6301 gtcgattggg tcgcccagtt tgccgctgcc gtgcaggcgg ctgacgcgca gattcccttc
     6361 accatacaaa tgcgcgcgga cttggtgagc gagcgcatgg ccgaggcgct acgcgatgcc
     6421 ggatgccgtg aggtctggat cggcgcggag agcggcagtc aacgggtgtt ggatgcgatg
     6481 aacaagggca ccaccgtggc cgagatccgc atcgctcgcg agcgcctgaa atccgtcggc
     6541 atccgcgtcg gatttttcat tcaattggga tacatgcatg agcagctgga ggacctccag
     6601 gcaacacgcg atctgctaca cgctgcgcgg ccggatgaga tcggcgtcag cgtctcgtat
     6661 cccttgccgg ggacaaagtt ttacgagctg gtcaaggcgc agctacgcga caagactcac
     6721 tggcaggaaa gcaacgatct tgaaatgatg tttcagggaa cctacgcctc ggacttttac
     6781 cgcgcagtca gaaatctgct gcacgatcag atagcgctgc agacggccgt cgatgcgccg
     6841 gacggcgaat cgcatggccg cgcgcgccgc gcgcttgatc agcgttggca ggatttgatg
     6901 gcgcaagagc tgcagtaccg ttcgccgctc gaccgttcgg ccgccgccgg atgaagacgc
     6961 ggctgccagg gccgaagacc ttgcagcgca tgctgcgcac cctcaccgag gcgctcgcgc
     7021 gagagcttgc ccggccgtcg ccgagcgcgc ccctgtggac ggatatcgag tggcggctcg
     7081 cgcacgcggt gtgcgcgatg cacggagttt cgcccctgct agcgcgctgc ctgccgtggt
     7141 caggccccgg cggttggcgt caatttcttg aagaacaaag attgcatacc aagaatagac
     7201 acgcccgcat cactcagttc ctgcgtgaga tcgatcagcg aatgggcgag gctggcgttg
     7261 ccgcggtcgc attgaagggc gtcgcgctgc acgcactcgg ggtgtatcga gcgggcgacc
     7321 ggccgatggc ggacatcgat ttgctggtgc ggccggccga tacgcagcgc agcgcccggg
     7381 cgctcgaatc gatggggttg cgcgagtgct accggacgtg gaaggaacgg gtgttcacgc
     7441 cggtggacgg gcatccgccc ggtgctttgg gggagcactc gaacaacaat atcaaattag
     7501 aattgcatga gcgcatcggc gagcggttgc cgtggcaaat cgtcgatatt tcggaacaca
     7561 tatttccccg cgattcgcgg ccgggcttga atgcctatcc atcgacggcc tccctgatga
     7621 tgcatctgct gctgcacgcg gccggatcga tggcgatgca agggctgcgg ctcatgcacg
     7681 ttcacgatct ggctcaattg tcgctgcgca tgacgcaccg ggactgggac gaactcctgg
     7741 cctatcgccc atgcgcgcag ccgctgtggt gggcgctgcc gccgctgaag ctgaccgcgc
     7801 gctactattc gtcgcaggtt ccgccgcggg tgctcgaagc cttggccgag cagtgcccgt
     7861 atctgttggc gcgggtcgcc gggagaaaga cactgtcgga cgtctcgtat tcctacccgt
     7921 gggtggacgc actccccggc atcgagtggt cgcaatcgct tcgcgaagtg ttgcagtatg
     7981 cgcacagccg cgtgcggccg accgcgaccc agatagcatt gcgcgagcaa ttcgcacaca
     8041 cgcagggcgg cggcctgcaa agcccgtggc accgcctttc gcagcgcaag cgcatggtgc
     8101 ggtggattac ctcaaacccg acgcggccgc tcaccatgca tgccgttgcg gcggcgctgg
     8161 cgccgccgcc ttgagcgcat ccatggcgcc agcggccgca cctgcggccg gtttgctggg
     8221 cgatacgccc gctcgaaatt actcgcgcaa gctggctcaa tttaatgctt tcgccgcacc
     8281 ggaactcaag cggctcattg ctgagatcgg cttgagtccg ggaatgcggg tgctggatgc
     8341 cggctgcgga acgggagagg cgctaccttg gctgcttgaa gcggcgccgt ccggacatgt
     8401 ggtcggcatc gatctgtcgg cggctcatgt gagtgaggcg cgccggcacg ccggcgcgca
     8461 aatcgaaatc ctgcaaggcg atcttgcgca agcgccgttg gcacccgcga gcatcgattt
     8521 tatatggtgt gtgaacacca tcaatcatct tcatgacccg cttcaagggg tggccagact
     8581 ggcggctttg ctgcgcccga acggccgaat cgccctaggc cagagttcgc tcctacctga
     8641 catgtatttt gcgtgggact cgaggctcga acgggtcacc aacgaggctg tgcgacacta
     8701 ttaccgcgag cgttacgcgc tgcaagagcg cgacctcgct gcggtcagag cgatcgtcgg
     8761 cgtactgcgc gcagccggtc tcaagagtgt cggcactaag acgctgctga tcgagcgaat
     8821 ttctccgctc gacaccgcca ccgaatgcta tttgcgcgag actctgttta gagatacgtg
     8881 gggagcgggc ctgcgtccct atttgtcgca cgaagatttt actgagctcg gttgcctgtg
     8941 cgacccggag cacccgaagt ttgctctgcg ccgtccggac tttcacttca tgcaaacttt
     9001 caccgtggcc atgggcacgc gttagagcag cttctcatac agcgccgaga agcgcgttgc
     9061 agtgtgatcg gcgtcctccc gtgtcgctcg ccgcagggcc tcgcgtgcga cggacatccg
     9121 caggtcttcg ttctcgagca gctgacggac cgccgcggcg aggccggccg aatcgccgac
     9181 ggaggccgat ttggcggcga acggcgccca ctcggcgata tggccgacgc gagtgccgac
     9241 ggtcggtacg ccggcgacag cggcctccag tgcgaccaag ggcccggttt cgtgacggga
     9301 ggagagcacc atgacgtccg ctagctccat caggggacgc aactgtctct gggtcagaaa
     9361 accatggaat tcgaccgagt cggacagctt caattgccgg gtcaacactt gtatttctcc
     9421 gagcagcgtg tcttctccga tgatatgcat ttcgaagcgc atgccggagt tcttgagcac
     9481 cgccaaggct cgcagcagcg tcggctgatc cttcacgcga ttcagactcg cgacgtggat
     9541 cagctgggct gggcggtcat tgcggcgagc gactggttgg cgcggcggcc acagcgacag
     9601 gtcgactcct agcggaatcc gctgggccgc aaatccgcag gcgttgagag atgcgatgac
     9661 aggcgcactc gccgccgtga tggcggatgc gtggcgcagc gcccagccct cgcgccaacg
     9721 gcccagccgc gtttgcgctc cgccgtacgc aatatcggga agcgacacca attcgccacc
     9781 ggcgatatgc accaaacttg gaatgccgag cgcgcggccg gcgatcaccg ctaccaaacc
     9841 acaaggcccc gaccagatgg cgtgcatcag gtcgaagctc cgcgtgcgat gcaatttata
     9901 aatcgcggca agggcgcgca gcgccgtggg ccgcactccg atgttgtgaa cacgcgcgcc
     9961 gcccaaattc caggcaccgg ctcgaagttc ctggcgtagg gcgatcacct gcacatcgtg
    10021 gtgcgcggat agccgagcga tcagggccaa caaggccgga attacccggt attcccccga
    10081 tcgatcgacg ccgcccggca ccaccagtgc gatgcgcatt cagcgggcat ccagcacaga
    10141 gcgcagcgcc tgtactgcct cccgcggatg gctgcctcgg cgaagctcaa agcagtcgag
    10201 gcgcgacata ttcctgcgga agtctgccca gggcggctgg cccgctgcgt agccctgagt
    10261 gtcggctaga ttggcgagca gcgacgcttt cgagatggct tgcagtaccg gccggtcgcc
    10321 ggcgctgtgc ggcaatacaa ataccgtgcc cacgatcttg agcggcgatc gagcaagtct
    10381 aaagccgccg cgccgcagat tgacctcgaa ctttttgacg ccgctgcggc gccggatcac
    10441 gggcgattgt cgaatcaccg ctgcaactcg cgaccggccc agccagcgca gagagtcggc
    10501 tcggatgtgc acatagttcg agacgccggt agccagcatc gtgtcgggac tgacaaatgc
    10561 gctatcctcc gagagaaaat caaagccgtc cagcaggcaa tgcaacgcca cagtggtctt
    10621 tccagagccg ctcggaccga tcagcagcaa agcgcggccg tgccggccga cgcacgccgc
    10681 gtgcagcgac accaaacgct gcgcgcgtgc ggccagcgtg aacacggcga attcgattaa
    10741 ttcataacgc gtgtggtagg gaaaacgcag catccgcggg ctgaccacca cgagcgccga
    10801 gtgggctcgg ggcgatatga ccgcgaagtc tgcttcaggt gtggcgccgg ccagcagagc
    10861 cgcgcctgaa agcatcttaa gcggcggcgg ctcgccgcga ccgaggcgct gtttcccgcc
    10921 ggtgagcagc aggctcaccc gaagctcgcc gtcgtagccc gacaaacggt gacgcgggag
    10981 gccttggtag gcacaattca ccaaacccaa caattcctcg ctgttgctgt cgaagcgaaa
    11041 tttcgcgccc aggagccgca tgcgctgggt gaagccggcg gatcgttcac caaacggatc
    11101 ggacaccaga tcgggtaaca attgagcgct cttgaggtac taacctcgtt agtaccctgt
    11161 cggtgcggtt atggtcgtat gacaacgctt tccggtccac aattgcacga tactcgggac
    11221 gcctttgata gcgtcgccgc ggactatgac ggcccgcgcg gcaataacga tcagattcaa
    11281 gagatgcgcc gcgacatgtg gcggtcgttg gatgctgcgt tcccaggccc cagccgcttg
    11341 atcgacctag gctgtggcac tgggctggac gccgtgcgca tggccaagcg cgggcacgta
    11401 gtgacggcga tggattggtc gggcgccatg gtgcagcgga ctcgcgaacg agctcaatcg
    11461 gaacacgtgc aggattggat cgaacctgtt gcgctcggcg cccacgagct gcggcacttg
    11521 aacggcgccg gacggtatga tggcgtctat tcgaatctcg gtcctctgaa ttgcgtgccg
    11581 gatcttggcg acgtgtcaca ggagtgcgct cgtctgctgc ggcccgatgg cgttttggta
    11641 tttacggtga tcggccgtta ttgcccttgg gaaattgcgc actatttgcg gctgcgtcgc
    11701 tgggcgagag cgaccgtcag gtttcaccgt caagtcgtgc ccatcagcat gaacgagcac
    11761 accatttggg ctcgctactt cgggccgcgc gaattttacc gggcattcag acagaacttc
    11821 cgtctcgagc accatcgggg gctctgccta ttttcgccgc ccccgtactt gacctgggtc
    11881 cgcgagcacc accctgtctg gcatgaacga ttgcagcgcc tcgaccggcg ggtctctcat
    11941 tggccgttgc tgcgtgcaat gggggaccat tttctgatcg tgatgaagaa gcggtaacga
    12001 cggcggtgcg cgaccgtgtc accatcttcg acgggctgca gggagtggcg gcgatcgccg
    12061 tggtgctttt gcatggctgc tcgatcttca atctcggctg gctgccggcg cacgcctact
    12121 tggcggtaga ttttttcttt ttgctgagcg gctatgtcat cgcccgtgca ttcgatgagc
    12181 ggctgcggca gggctggctg tttggatttt tgcagcgtcg cctgattcgg ttgtacccgt
    12241 taatcgtcgt cggttctctg gtcggcttta ttgaaatgat cattcacggc gtgctcacgc
    12301 atagcgtgag catttctcag gcc
//
LOCUS       DASWZT010000268         2572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_40884, whole genome shotgun sequence.
ACCESSION   DASWZT010000268 DASWZT010000000
VERSION     DASWZT010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40884"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..664
                     /locus_tag="VGE92_07205"
     CDS             5..664
                     /locus_tag="VGE92_07205"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY1313646.1"
                     /translation="MDDVAEMPRETQNKILRVLVDQTFQRVGGSTKVAVDVRIVSSTA
                     RNLEAEIAEGKFREDLYHRLSVVPIRVPPLAERREDIPGLVEYFMDQISQATGLPKRR
                     IGEDAMAVLQSHSWPGNVRQLRNNIERLMILAGGDPDAVMDASMLPPDVGSMVPQMPN
                     GNGGEHLMGLPLRDAREVFEREYLVAQISRFGGNISRTAEFVGMERSALHRKLKALGI
                     G"
     gene            727..1584
                     /locus_tag="VGE92_07210"
     CDS             727..1584
                     /locus_tag="VGE92_07210"
                     /inference="COORDINATES: protein motif:HMM:NF005209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HEY1313647.1"
                     /translation="MSRIAYVNGRYLSRAHAMVSVEDRGYQFADGVYEVCEVRGGRLV
                     DERRHMARLDRSLNELRIARPMSSAALGVVLRETVRRNRVRDGIVYLQITRGVARRDF
                     PFPRADTKPSMVVTARSNDLAHLEHMAEEGIAVVTVPDIRWQRVDIKSVALLPNVLAK
                     QAARDRGAREAWLVDAQGQITEGASSNAWIVSRDGKLITHPLGRDILGGITRSVLMDV
                     LKTQGLAFEERAFTVDEAYAAHEAFITSASQIVLPVVSIDGKPVGNGAPGLITTALRR
                     DYHRHAEIA"
     gene            1810..2070
                     /gene="hfq"
                     /locus_tag="VGE92_07215"
     CDS             1810..2070
                     /gene="hfq"
                     /locus_tag="VGE92_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007114649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEY1313648.1"
                     /translation="MAAERAQNLQDTFLNHVRKAKIPLTIFLVNGVKLQGVVTWFDNF
                     CVLLRRDGHSQLVYKHAISTIMPGHPIQLFEGGEEPAAGEKV"
     gene            2071..>2572
                     /locus_tag="VGE92_07220"
     CDS             2071..>2572
                     /locus_tag="VGE92_07220"
                     /inference="COORDINATES: protein motif:HMM:NF024566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313649.1"
                     /translation="MEERQRDKRADRLSLTSGGETGRAIVIEPWLKRVTPRGGDARAA
                     ETRSPEARLAEAVGLAGAIDLTVVQSGLVTLNQTRPATYIGKGKVDEIAGLVKSLSVS
                     VVVMDCPVSPVQQRNLEKAWNAKVIERTGLILEIFGRRARTSEGALQVELAHLNYQKT
                     RLVRAWT"
BASE COUNT          502 a          744 c          845 g          481 t
ORIGIN      
        1 gtttatcgac gatgtcgccg agatgccgcg cgagacgcag aacaagatcc tgcgcgtgct
       61 ggtcgaccag acctttcagc gggtcggcgg cagcaccaag gtcgcggtcg atgtgcgcat
      121 cgtgtcgtcg accgcgcgca acctcgaagc ggagatcgcg gaaggaaaat tccgcgagga
      181 tctctaccat cgtttatcgg tggtgccgat ccgcgtgccg cctttggccg agcggcgcga
      241 ggatatccca ggtctggtgg aatatttcat ggaccagata tcgcaggcta ccggcctgcc
      301 gaagcgccgc atcggcgaag acgccatggc ggtactgcaa tcgcacagtt ggccaggcaa
      361 tgtccgccag cttcgcaaca atatcgaacg gttgatgatt ctggccggtg gcgatccgga
      421 cgcggtgatg gacgccagca tgttgccgcc ggatgtcggc tcgatggtgc cgcagatgcc
      481 caatggcaat ggcggcgagc atctgatggg cctgccgctg cgcgatgcgc gcgaagtgtt
      541 cgagcgggag tatctcgtgg cgcagattag ccgctttggc ggcaatatat cgcgcaccgc
      601 cgaattcgtc gggatggagc gctcggcgct gcaccgcaag ctcaaggcac tcgggatcgg
      661 ctagcgcgtg atcccgaaaa gtgggaaccg gttttcggat cagatcacgc gcaaggaata
      721 tacgtcatgt cgcgcatcgc ttacgtcaat ggccggtatt tgtcgcgcgc gcacgccatg
      781 gtttcggtcg aagaccgcgg ttatcagttc gccgacggcg tctacgaagt ttgcgaggtg
      841 cggggagggc ggctggttga cgaacgtcgc catatggcgc ggctcgatcg ctcattgaac
      901 gaattgcgca tcgcgcgccc gatgtcgtcg gcagcgctcg gcgtggtgtt gcgcgaaacc
      961 gtgcggcgca atcgcgtgcg cgacggcatc gtgtatttgc aaatcactcg cggcgtcgcg
     1021 cggcgtgatt ttccgtttcc gcgggcggac acaaagccgt cgatggtggt gaccgcgcgc
     1081 agcaacgatc tcgctcatct cgaacacatg gcggaggagg gcattgccgt ggtgaccgtg
     1141 cccgatatcc gctggcagcg ggtcgacatc aaatcggtgg cgctgttgcc gaatgtactg
     1201 gccaagcaag ccgcgcgcga tcgtggcgcg cgtgaggcct ggctggtcga tgcccagggc
     1261 caaatcacgg aaggcgcctc gtcgaatgcc tggattgtca gccgcgacgg taaactcatc
     1321 acgcatccgc tcggccgcga tattctcggc ggcatcaccc gctcggtttt gatggatgtg
     1381 ctcaagacgc aggggctggc gttcgaggag cgggccttca cggtcgatga ggcctatgcc
     1441 gcgcacgagg ccttcattac ctcggcgagc cagatcgtgc tgccggtggt cagtatcgac
     1501 ggcaagccgg tgggcaatgg cgcccccggg ctaatcacaa cggcgctgcg ccgcgactat
     1561 caccggcacg ccgaaatcgc ctgattcggc ccgccgaaga cggcgaaata tcaattattt
     1621 cagccgtttg gtgaggtgga tgccaacttg tgcttgcgcc cgggaaccgc ctgccatcta
     1681 atagcagcct cctgagccct gacgtatctg cgtggggcta agcgttcgca tggtctggaa
     1741 ctcccatgcg agggactcgg gcaacaaagg tctcgcgcgc tagacgcggg caaaaaatgg
     1801 aacacggcca tggcagccga acgagcacaa aatctacaag acacatttct caatcacgtc
     1861 cgaaaagcaa agatcccgct caccattttt ctggtgaacg gggtaaagtt gcagggcgtg
     1921 gtgacctggt tcgataattt ctgcgttttg ttacgacggg acggacattc ccagctcgtc
     1981 tacaagcacg cgatctcgac catcatgcct ggtcacccga tccagctgtt cgaaggcggc
     2041 gaagagcccg cggctgggga aaaggtctga ttggaagaac gccaacgtga caagcgcgcc
     2101 gaccgtctca gcctgacttc gggcggagag accggccgcg ccatcgtcat tgaaccgtgg
     2161 ctcaagcgtg tgacgccgcg cggcggcgat gcgcgcgcgg cggagacgcg ttcgcccgaa
     2221 gcgcgcttgg cggaggcggt cggacttgcc ggagccatcg atctcaccgt ggtgcaatcg
     2281 ggattggtca cgctcaatca aactcggcca gcgacttata tcggcaaggg taaggtcgat
     2341 gagatcgccg gactggtgaa gagcctctca gtgtcggttg tggtgatgga ttgcccggtg
     2401 tcgccggtgc agcagcgcaa tctggaaaag gcctggaatg cgaaagtcat cgaacgcacc
     2461 ggtttgatcc tggaaatttt cggccggcgc gcgcggacca gcgaaggcgc gctgcaagtc
     2521 gagctcgcgc atctaaatta tcaaaagacg cggctggtac gcgcctggac cc
//
LOCUS       DASWZT010000269         1613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_79525, whole genome shotgun sequence.
ACCESSION   DASWZT010000269 DASWZT010000000
VERSION     DASWZT010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79525"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /locus_tag="VGE92_07225"
     CDS             complement(<1..1275)
                     /locus_tag="VGE92_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY1313650.1"
                     /translation="MKLYRVVAEELMDLIRGGTLRPGDQLPSVRVLSDKRRVSPSTVL
                     RAYEILEADRLIEARSRSGYYVAQRSQPAAEPRASTPKSSSTRLAVSDLVFQTLEASR
                     NRKVAPLGSAFPSPALFPWSKLARYLGSGARHMDPWNTVESLPPGSTALRRQIARRYL
                     QQGIAVGMEQIIVTAGALEALNLALQTVTRPGDTVAVEAPTFYGCLQAVERLALNVIE
                     IPTHPADGVDLAALAKALAKHPIRACWFMTTLQHPTGAIVPTERKRELVNLLASHDVA
                     LIEDDAYAELQFSAAPSLPAKAYDKRGLVLHCGSFSKCLAPGYRLGWVAAGRFAGDVA
                     RRKIESSLATSLPVQLAIADMLARGGYDSHLVRLRGALASQQSAALASLRRHFPAGYR
                     VAQPGGGYFLWIECGAGVDSRDLHRLALAAGIG"
     gene            complement(1309..1509)
                     /locus_tag="VGE92_07230"
     CDS             complement(1309..1509)
                     /locus_tag="VGE92_07230"
                     /inference="COORDINATES: protein motif:HMM:NF022587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2909 domain-containing protein"
                     /protein_id="HEY1313651.1"
                     /translation="MQALIVGFLVLIVASLGHALSSLASGPEKAALMAQALTMRISLS
                     IALFVLLLVGYHFGWIAPHAVH"
BASE COUNT          310 a          516 c          527 g          260 t
ORIGIN      
        1 gccgataccc gcagcgagcg caagccgatg caggtcgcgc gagtcgacgc cggcgccgca
       61 ttcgatccac aggaaatagc cgccgccggg ctgcgctaca cgataacccg cgggaaaatg
      121 ccgccgcagc gatgcgagcg ccgcgctctg ttgcgaggcg agagcgccgc gcagccggac
      181 caagtgtgaa tcgtagccgc cgcgcgccag catgtcggcg atggcaagtt gcaccggcag
      241 gctcgttgcc agcgaggatt caatcttgcg ccgcgcgaca tcgccggcga atcgtcccgc
      301 cgccacccag ccgagacgat atccgggtgc caggcatttg ctgaaggagc cgcagtgcaa
      361 gaccagtcct ctcttgtcgt acgccttggc cggcaacgac ggcgccgctg aaaattgcaa
      421 ttcggcgtac gcatcgtcct cgatcagcgc gacgtcgtgg cttgcgagca gattcaccaa
      481 ctcgcgcttg cgttcagtgg gcacgatcgc ccccgtcgga tgctgcaatg tggtcatgaa
      541 ccagcacgcg cgaatcggat gcttggcaag cgccttggcc aaggcggcca gatcgacgcc
      601 gtcggccgga tgcgttggga tctcgatcac gttgagggcg aggcgttcca cagcctgcag
      661 acagccatag aatgtcggtg cttcgaccgc gaccgtgtca cccgggcgcg tcaccgtttg
      721 cagcgcgaga ttgagcgcct cgagcgcgcc cgccgtcacg atgatctgtt ccatgccgac
      781 ggcgattcct tgctgcaaat aacggcgtgc gatctgtcgg cgcagcgccg tgctgccggg
      841 aggcaggctt tccaccgtat tccaggggtc catgtgacga gcgccgctgc cgaggtatct
      901 ggccaacttg ctccagggga acaacgcggg gctggggaac gccgatccca agggtgcgac
      961 tttgcgattg cgggacgcct ccaaggtttg aaacaccagg tcgctcacgg ccaagcgcgt
     1021 ggaactcgac ttgggcgtcg aggcgcgagg ttccgcggcc ggttgagagc gctgcgccac
     1081 gtagtatccg gagcgggagc gcgcttcgat gagccgatcc gcttcgagaa tttcgtaagc
     1141 gcggagcacc gtggacggac tgacccgccg cttatcggac aacactcgaa ccgagggaag
     1201 ctggtcgccg ggccgcaacg tgccgccgcg tatcagatcc atcagctctt cggccaccac
     1261 tcgatacagc ttcatgccgc gaacactacg gccgcggcaa tctcgtggtc aatgcacggc
     1321 gtgcggtgcg atccagccga aatggtagcc aacgagcagc aacacgaaca gcgcgatgga
     1381 gagtgagatg cgcatggtga gggcctgagc catgagcgcg gctttctccg ggcccgatgc
     1441 caaggacgac agcgcgtgtc cgaggctggc tacgatcagc accagaaatc cgacgatcag
     1501 ggcttgcatg cgtcctcctt ggctgatttg ctctcgcgcc gaatggtggc aacgaccgtc
     1561 gcaagcgcca cgccgagcac cgagaccatc gcgaccatga ggaataggag acc
//
LOCUS       DASWZT010000270         1783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_79528, whole genome shotgun sequence.
ACCESSION   DASWZT010000270 DASWZT010000000
VERSION     DASWZT010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79528"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1701)
                     /locus_tag="VGE92_07235"
     CDS             complement(<1..1701)
                     /locus_tag="VGE92_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS2/Smr-associated SH3 domain-containing
                     protein"
                     /protein_id="HEY1313652.1"
                     /translation="MQGVVHGASSSGQTVFVEPLETIEQNNDLVRLLDEEQAEIHRVL
                     LEMTRQIAESAEAILAAAETLAELELQFAKARFGEDYNCVAVSLSGDGSGVGEDRLLL
                     HRARHPLLERNLKLKNAKIVPVSIEMEGDHRQLVITGPNTGGKTVSLKTLGLLALMAQ
                     SGIPVPADRADMPVFDAILADIGDYQSIEQNLSTFSAHVTNIDFISRTATANSLVLLD
                     ELGSATDPEEGAALAVAIAEHFRQVGCLSVISTHHTSLKVYGANTAGVINASVGFDEA
                     TLQPTYELKIGVPGASAGINIAQRLGLNPAIIASARSRLGSQTQDVGRFLDRLHSDLR
                     ELESERARMQLREQELEREKSRLASEGRKEQQSKIREMEAKLETLFRDFEYHARETVN
                     AVQDRAAAQKLSKEAERRIAKMRREFREQFDATVVAHATGADRGDPNAQPSLVKHVSE
                     GDTVKLKSMGRAAVVKKKIGDDHFDVEIGSMKMRIHREDIAEMLATAQGNSQPLSSPV
                     ESARSRGITVKLQNEFSDLNTPSEINVIGRTVDEATGEVEKFIDRAFLAGMPRVRVVH
                     G"
BASE COUNT          292 a          533 c          575 g          383 t
ORIGIN      
        1 gccgtgtaca acacgcacgc gcggcattcc ggcgagaaac gcgcggtcga tgaatttctc
       61 gacttcgccg gtggcttcgt ccacggtgcg cccgatgacg ttgatttcgc tgggcgtgtt
      121 caggtcgctg aattcgtttt gcagcttgac ggtaattccc cgcgagcgcg ccgattcgac
      181 gggcgatgac agaggctgcg agtttccttg ggcggttgcc agcatctcgg cgatgtcttc
      241 gcgatgaatg cgcatcttca tcgagccgat ctcgacgtca aaatgatcgt ctccgatttt
      301 ctttttgacg acagcggcgc ggcccatcga cttgagcttg acggtgtcgc cctcggagac
      361 gtgtttcacc aggctcggct gcgcgttggg atcgccgcga tcggcgcccg tggcatgcgc
      421 gacgactgtg gcatcgaact gctcgcgaaa ttcccgccgc attttggcga tgcggcgctc
      481 cgcctctttc gataacttct gcgcggcggc gcggtcctgc acggcgttca cggtctcgcg
      541 cgcgtggtac tcgaagtcgc ggaacaaagt ttcgagcttc gcctccatct cgcgaatctt
      601 ggactgctgt tctttgcggc cctcggatgc gagccgcgat ttctcgcgct ccagctcttg
      661 ttcgcggagt tgcatgcgag cgcgctcgga ttccagttcg cgcaggtcgg agtgcaagcg
      721 atcgagaaag cgcccgacat cctgcgtctg cgagcccagt cgcgagcgcg ccgatgcgat
      781 gatggcggga ttcaatccga gccgctgcgc gatgttgatg ccggcggaag cgccggggac
      841 gccgatcttt agttcgtaag ttggctgcaa ggtggcttcg tcgaagccga ccgaggcgtt
      901 gatgactcct gccgtgttcg cgccgtaaac cttgagcgac gtgtggtgcg ttgagatcac
      961 gctcaagcac ccaacctgac ggaagtgctc ggcaatggcc acggccagcg cagcgccttc
     1021 ttctggatcg gtcgcagacc caagctcgtc gagcagcacc agcgaatttg cggtcgcggt
     1081 gcgcgagatg aaatcgatgt tggtcacgtg cgccgaaaag gtcgagagat tctgttcgat
     1141 cgactggtaa tcgccgatgt cggcgagaat cgcgtcgaag acgggcatgt cggcgcggtc
     1201 ggcgggcacg gggatcccgg actgggccat gagcgcaagc aggcccagcg ttttcaggga
     1261 gaccgtttta ccgccggtgt tcggtccggt gatcaccagt tggcgatggt cgccttccat
     1321 ctcgatgctg acggggacta tcttcgcgtt cttgagtttc aggttgcgct caagcagtgg
     1381 atgccgtgcg cggtgaagca gcaggcggtc ttcgcccacc ccggacccat ccccggaaag
     1441 gctaacggcg acgcagttgt agtcttcgcc aaagcgcgcc ttggcaaatt gcagctcgag
     1501 ctcggcaaga gtctcggcgg ccgcgagaat cgcttcggcg gattccgcga tctggcgcgt
     1561 catctccagc agaacgcgat ggatctcggc ttgttcttca tccagcaggc ggaccaggtc
     1621 gttgttctgc tcgatggttt cgagcggctc cacgaagacg gtctgcccgc tggaactggc
     1681 gccgtggacc acgccctgca cgcggcgttt ctgctcgact ttcaccggta tgacaaagcg
     1741 ctcgccgcga atggtgatga gttcatcctg cacggtgccg cct
//
LOCUS       DASWZT010000271         6467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_94160, whole genome shotgun sequence.
ACCESSION   DASWZT010000271 DASWZT010000000
VERSION     DASWZT010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94160"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..1464
                     /locus_tag="VGE92_07240"
     CDS             142..1464
                     /locus_tag="VGE92_07240"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY1313653.1"
                     /translation="MSFSDLGLLPELLRAVADKGYDSPSPIQMQAIPAVLAGRDVLAG
                     AQTGTGKTAGFVLPILQRLAADNAHGTSRAPRALVLTPTRELAAQVAQSARDYGKYMQ
                     LRTYQVFGGVSINPQISELRSGCDILVATPGRLLDLAQQRVADLSKVQVLVLDEADRM
                     LDMGFINDIRKVIKLLPPKRQNLLFSATYSDDIRSLAERLLHDPLSIQVAPRNAPIEL
                     VEQRAYRVQKEQKRHLLVHLIQEGQWRQVLIFTRTKHGANRLTQQLESAGIDAAAIHG
                     NKSQAARVKALEMFKRGQVTALVATEVAARGLDIKELPQVVNYELPNVPEDYVHRIGR
                     TGRAGSAGEAISLVSSDESGLLRDIERVLRRSIPTVPTPVFKIVESAPQPATRGDRPQ
                     GDRQGNRPAQGQHRAGQGHHGARDSRRTPASSGSGTRSFGQQRRGRSR"
     gene            complement(1461..2348)
                     /gene="galU"
                     /locus_tag="VGE92_07245"
     CDS             complement(1461..2348)
                     /gene="galU"
                     /locus_tag="VGE92_07245"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEY1313654.1"
                     /translation="MTHNIVRTVVFPVAGRGTRFLPVTKASPKEMLPIVDKPLIQYAV
                     DEALSAGADTLIFITGSSKRAIEDHFDSNPELENVLQTQGKQDLLDILRGILPSWASC
                     VYIRQPAPLGLGHAVLCARPVVGDAPFMVHLADDLIDAEVPCLKQMVQVFETHRGSVL
                     GVQDVPRNDTDKYGIVTLERKLANRIGQMSNIVEKPKPDKAESTLAVVGRYLLTPAIF
                     DDLVKIGKGAGGEIQLTDGIARLMRREPVYAFEFEGKRYDCGSKLGYLQATVEYALKH
                     PSLGEGFAAYLKALAPTLP"
     gene            complement(2402..3184)
                     /locus_tag="VGE92_07250"
     CDS             complement(2402..3184)
                     /locus_tag="VGE92_07250"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1313655.1"
                     /translation="MTELPLLTWILVVAALVLGILAGHFGWGKRWPASFSKLHPDYLT
                     GLDYLVTEQPDRALDMFLKLMDANADTIETHFALGTLYRRRGEVERAIRIHQNLLARQ
                     ALAPEHREQALLALAQDYLRAGLLDRAEGLFQQVSEVARLRASALDALLSELVTQAES
                     RDFSELKRLVFAAVAADLVDAAPLRGAIEKVLLQDPTLQAMWRAAPEQYSRTAHEIGA
                     LLAHAEKYRCSECGFSGRHFYWHCPACHSWDSFEAYAIVKLR"
     gene            complement(3185..3487)
                     /locus_tag="VGE92_07255"
     CDS             complement(3185..3487)
                     /locus_tag="VGE92_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004815605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEY1313656.1"
                     /translation="MTKSELIEIISAKQKHLPAKDVELAVKQLLEIMSDALATGERIE
                     IRGFGSFSLHFRPPRQGRNPKTGEAVALAGKHVPHFKPGKDLRERVNDGADQPIRD"
     gene            complement(3568..5238)
                     /gene="rpsA"
                     /locus_tag="VGE92_07260"
     CDS             complement(3568..5238)
                     /gene="rpsA"
                     /locus_tag="VGE92_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016568693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEY1313657.1"
                     /translation="MTESFAELFEQSIASQRIRPGTILNGLIVEVGQDYVIVNVGLKS
                     EAVIPSDQFKNEKGEIEVVVGETVEVALDSVEDGSGETRLSREKAKRARTWTRLETAF
                     EKQEVVIGIITGRVKGGFTVEIDNVRAFLPGSLVDVRPVRDPSYLENKPLEFKVIKLD
                     QKRNNVVVSRRAVVEQEYSAERSALLDNLQEGAAVKGVVKNLTDYGAFVDLGGIDGLL
                     HITDMAWKRVKHPSEVVKVGEEIDVRILKFDRERQRVSLGLKQLGADPWQNIARRYPA
                     NTRLFGKVTNIADYGCFVEIEEGVEGLVHVSEMDWTNKNVNPAKVVHVGQEVEVMVLD
                     IDEERRRISLGVKQCKTNPWKEFAENYNRGDKVSGQIKSITDFGIFIGLTGGIDGLVH
                     LSDISWDMPGEEAVRSYQKAQQVDAMVLSIDPERERISLGIKQLAKDPFSAYIAEHPK
                     GSIVTGVVREVDAKGAIIDLGNGVDGQLRASELSRDRVEDARLLLKVGEEVEAKFTGV
                     DRKGRSISLSIKAKDIHEEQEAMQNYRTDSPTGTSLGDLLKEQISGQD"
     gene            complement(5340..6020)
                     /gene="cmk"
                     /locus_tag="VGE92_07265"
     CDS             complement(5340..6020)
                     /gene="cmk"
                     /locus_tag="VGE92_07265"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEY1313658.1"
                     /translation="MSRVPIMTIDGPSGSGKGTVSRAAARALGWALLDSGALYRLVAL
                     AGRLQGVSLSDGPKLAQLAGHLDLKFGSDAAGGELVSLNGQEVTGAIRSEQAGNDASK
                     VAALPEVRAALLDRQRRFAVPPGLVADGRDMGTVVFPQAEVKIFLTASATERALRRHK
                     QLKEKGVAATLAALSQEIGERDLRDMNRAASPLVASADAVLLDTTGMSVDAVVERVLG
                     VARGRLSP"
     gene            complement(6017..>6467)
                     /locus_tag="VGE92_07270"
     CDS             complement(6017..>6467)
                     /locus_tag="VGE92_07270"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="catalyzes the formation of
                     5-O-(1-carboxyvinyl)-3-phosphoshikimate from
                     phosphoenolpyruvate and 3-phosphoshikimate in tryptophan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEY1313659.1"
                     /translation="LRVHASALRGVIVPAQIVPLAIDELPVLFVAAACAAGETLVTGA
                     EELRVKESDRIAAMSAGFAVLGVAHQMLPDGMRIEGRGSGAAFGGGAVDSFGDHRIAM
                     SFAIASLRCARPIAIHDVANVATSFPDFADLARSVGLDVRESPEAPP"
BASE COUNT         1215 a         2111 c         1892 g         1249 t
ORIGIN      
        1 gatacggtcg gtactcggtc agtgccacct tgagctcgcg gtccgtcctt gacgcacttc
       61 gcctttttac ccccctcgct gctgaccgct gccgaagcct ttgcttctgt cgtgcgagtc
      121 ttcaattctt aaggattttt aatgtcattt tccgatctcg ggctattgcc cgaactgctg
      181 cgtgccgtcg ccgataaggg ctacgattcg ccttctccca ttcaaatgca agcgatcccg
      241 gccgtactcg ccggccgcga cgtactcgcc ggcgcgcaga ccgggaccgg caaaaccgcc
      301 ggcttcgtcc tgccgatatt gcagcgcctg gcggccgata acgcccacgg taccagccgc
      361 gcgccgcgcg ccctggtgct gacaccgacc cgcgagctgg cggcgcaggt cgcgcaaagc
      421 gcgcgcgact acgggaaata catgcagctg cgcacttatc aggtgttcgg cggcgtgagc
      481 atcaacccgc aaatttccga gctgcgctcg ggttgcgata ttttggtggc aacgcccggc
      541 cgccttttgg acctggcaca acagcgcgtg gcggatctgt ccaaggtgca ggtcttggtg
      601 ctcgatgagg ccgatcgcat gctcgatatg ggtttcatca acgacatccg caaagtgatc
      661 aaattgctgc cgccgaagcg ccagaacctg ctgttctccg cgacctactc ggacgacatc
      721 cgtagcctgg ccgaacggct gctgcacgat cccctgtcga ttcaagtggc gccgcgcaac
      781 gccccgatcg agttggtcga gcaacgcgcg tatcgcgtac aaaaggaaca gaagcgccat
      841 ctattggtac atttgatcca agaaggacag tggcgccagg tgctgatctt cacgcgcacc
      901 aagcacggcg cgaaccgtct cacccagcag ctggaaagcg ccggcatcga tgccgcggcg
      961 atccacggca acaagagcca ggcagcccgc gtcaaggcac tcgagatgtt caagcgcgga
     1021 caagtcaccg cgctggtcgc taccgaagtc gcggcccgcg gtctcgatat caaggaactg
     1081 ccgcaagtcg tcaactacga attgccgaac gtgccggaag attacgtgca tcgtatcggc
     1141 cgcaccgggc gcgccggctc ggcgggcgaa gcaatttcgc tggtgtcgag cgatgaatcc
     1201 ggactgctgc gcgacatcga gcgagtcctg cgccgcagta ttcccactgt gccgaccccg
     1261 gtattcaaaa tcgtcgaatc ggcgccgcag cctgcgacgc gcggcgatcg tccgcaggga
     1321 gatcgtcaag gcaaccgtcc ggcgcaaggc cagcaccgcg cgggccaagg ccaccacggt
     1381 gcccgcgatt cgcggcgtac accggcgagc agtggatccg gcacgcgcag cttcggccaa
     1441 cagcgccgcg ggcgctcccg ctagggcagc gtcggggcca gggccttcag gtaagcggcg
     1501 aatccctcgc cgaggctcgg atgcttgagg gcatattcaa cggtcgcctg caagtatccg
     1561 agcttgctgc cgcagtcgta gcgcttgcct tcgaattcga acgcatacac gggttcgcgc
     1621 cgcatcagcc gggcaatgcc gtcggtgagt tgaatctcgc cgccggcacc tttgccgatt
     1681 ttcacgaggt cgtcgaatat agccggcgtc aacaagtagc ggccgaccac ggccagggtc
     1741 gattccgcct tgtccggttt gggcttctcg acgatgttcg acatctgacc gatgcggttc
     1801 gcgagcttgc gctccaacgt cacgatgccg tacttatccg tgtcgtttcg cggcacgtcc
     1861 tggaccccga gcacgctgcc gcggtgcgtc tcgaacacct gcaccatctg cttgagacag
     1921 ggcacctcgg catcgatcaa atcgtccgcg agatgcacca tgaacggcgc atcgccaacc
     1981 accggcctcg cgcaaagcac cgcatgaccc aagccgagcg gcgccggttg acgtatgtac
     2041 acgcatgacg cccagctcgg cagaatgccg cgcaatatat cgagcaagtc ctgtttaccc
     2101 tgggtctgca ggacgttctc gagttcgggg ttgctgtcga aatgatcctc gatagcgcgc
     2161 ttcgaactgc cggtgatgaa aatcaaggtg tcggcgccgg cggagagcgc ttcatccacc
     2221 gcatactgga tcagcggctt gtcgacgatc ggcagcattt ctttcgggct ggctttggtc
     2281 accggaagaa atcgcgtgcc gcgtccggct acggggaaga cgacggttct gacgatgtta
     2341 tgtgtcattg cgaaataata acggatttcg gcgccgcgtg ccgtcggaca aagcgcacaa
     2401 attagcgaag tttaacgatg gcataagcct cgaagctatc ccaggaatga caggccgggc
     2461 aatgccaata aaagtgacgt ccggaaaatc cgcactcgct gcaccgatac ttctcggcgt
     2521 gcgcgagcag cgccccgatc tcgtgcgcgg tgcgcgaata ctgctcgggc gccgcgcgcc
     2581 acattgcctg caacgttgga tcctgcaaca ggactttttc gattgcgccg cgcagcggcg
     2641 ccgcgtcgac caagtccgcc gcgaccgccg cgaataccag gcgcttcagt tcgctaaaat
     2701 cgcgcgactc ggcctgggtt accaactcgg acagcagcgc gtcgagagcg ctggcgcgca
     2761 gccgcgccac ctcgctcact tgctggaaca agccttcggc acgatcgagc aggcccgcgc
     2821 gcaggtagtc ctgtgcgagt gcgagcaacg cctgctcgcg atgctccggg gcgagcgcct
     2881 ggcgcgccaa caagttctga tggatgcgga tggcgcgctc gacctcgccg cgccggcgat
     2941 acagcgtccc gagcgcgaaa tgcgtctcga tggtgtcggc gttggcatcc atcagcttca
     3001 agaacatgtc gagcgcgcga tcgggctgct ccgtcaccag gtaatcgagt ccggtgagat
     3061 agtcgggatg cagcttgctg aacgaggccg gccagcgttt gccccagccg aagtgtccgg
     3121 ccagtatccc aaggaccaac gccgccacca ccaagatcca agtcagcagc ggtagctcgg
     3181 tcacctaatc gcggatcggc tgatccgcac cgtcgttgac tcgttcgcgc aagtccttgc
     3241 ccggtttgaa atgcggtacg tgtttgccgg cgagcgccac ggcttcgccg gtcttcggat
     3301 tgcggccctg ccggggcggc cggaaatgca gcgaaaagct gccgaagccg cgaatttcga
     3361 tgcgctcgcc cgtcgccagc gcatcgctca tgatttccag cagctgcttg acggcaagtt
     3421 cgacgtcttt cgccggcaag tgcttctgtt ttgctgaaat aatctcaatg agttccgatt
     3481 tcgtcattat cgatccgtgg gaatcattag acatgcttat agagagtaaa cgcggcgcgt
     3541 acgcggcgcc gcgttcaacc tttcccctca atcctgaccg gagatttgct ctttgaggag
     3601 gtctcccaaa ctggttccgg tcggcgaatc ggtccgataa ttctgcatgg cttcttgttc
     3661 ctcgtggatg tcctttgcct tgatcgacag cgagatgctg cggcccttgc gatcgacgcc
     3721 ggtgaatttc gcctcgacct cttcacccac cttgagcagc agacgtgcat cttccacgcg
     3781 atcgcgcgac aactccgaag cccgcaattg accgtcgacg ccgttgccaa gatcgatgat
     3841 cgcccctttg gcatccactt cgcgcaccac gccggtgacg atgctgccct taggatgctc
     3901 ggcaatgtag gccgagaaag gatccttcgc gagctgcttg attcccaagc tgatgcgctc
     3961 gcgttccgga tcgatcgaca gcaccatggc atcgacctgc tgcgccttct gatagctgcg
     4021 aacagcctct tcgcccggca tgtcccacga gatgtccgac agatgcacca agccgtcgat
     4081 gccgccggtc aggccgatga aaataccgaa atccgtgatg gatttgatct gcccgctgac
     4141 cttgtcgccg cggttgtaat tctccgcgaa ttccttccac ggattggtct tgcattgctt
     4201 cacgccgagg ctgatgcggc ggcgttcctc gtcgatatcg agcaccatga cttcgacttc
     4261 ctggccgacg tgcacgactt tggcgggatt gacgttcttg ttggtccagt ccatctccga
     4321 gacgtgcacc agtccctcca caccctcttc gatttcgacg aagcagccgt aatcggcgat
     4381 gttggtcacc ttgccgaata gacgcgtgtt ggccggataa cgccgtgcga tgttctgcca
     4441 cgggtccgcg cccaactgct tgagaccgag cgagactcgt tgacgttcgc gatcgaattt
     4501 caaaatccgc acgtcgatct cttcgcccac tttgaccact tcggaaggat gcttgacacg
     4561 cttccacgcc atatcggtga tgtgcagcaa tccatcgatg ccgcccaagt cgacgaaggc
     4621 gccgtagtcg gtgagattct tcaccacacc cttgacggcc gcgccctcct gcaaattgtc
     4681 caaaagcgcg gagcgctcgg cgctgtattc ctgctcgacc accgcgcgcc gcgacacgac
     4741 cacgttgttg cgcttctgat cgagcttgat cactttgaat tcgagcggct tgttctccag
     4801 gtatgacgga tcacgcaccg gccgcacatc gaccaaggaa cccggcaaga aggcgcgcac
     4861 attgtcgatc tcgacggtga acccaccctt gacccggccg gtgatgatgc cgatcacgac
     4921 ttcctgcttc tcgaaggccg tctcgagacg cgtccaggtg cgggcgcgct ttgccttctc
     4981 gcgcgacagc cgggtttcgc ccgagccgtc ctcgacgctg tccaaggcga cttcgacggt
     5041 ctcgccgacg acgacctcga tctcaccttt ctcattcttg aattggtcgg acggtatgac
     5101 cgcttcggac ttcaatccga cgttgacgat gacgtagtcc tggcctactt cgacgatcaa
     5161 tccgttgagg atggtgccag gacgaatgcg ctggctcgca atgctctgtt cgaacagttc
     5221 cgcaaaactt tctgtcatgt gttctctact attcccgggc acttatcccg tgtgcctcag
     5281 ggttaccata aaatagcggc gctcaatcca tgaaaaccgc aacccaaacc tcttaccaac
     5341 tatggcgaca gacgtccgcg ggccacaccc aagacgcgtt caaccactgc atcgacggac
     5401 atccccgtcg tgtccagtag gaccgcatcc gcgctcgcga caaggggcga ggcggcgcga
     5461 ttcatgtcgc gtaggtcgcg ctcccctatc tcctgcgaaa gggcggcaag ggtagcagca
     5521 accccctttt ctttcaactg cttatggcgc ctcaaggccc gttcagtcgc gctcgccgtg
     5581 agaaaaatct taacctccgc ctgggggaat accacggttc ccatatcgcg cccgtcggcc
     5641 accaaaccgg ggggtaccgc aaatcgccgc tgacgatcca agagcgccgc acgaacctcg
     5701 ggcaaggccg ccactttgga ggcatcattc cccgcctgtt cgctccgaat ggcgccggtg
     5761 acctcctgtc cgttgagtga gaccagctcg ccgcccgctg catcggaacc gaatttgagg
     5821 tcgaggtgcc cggccaattg ggcgagtttc gggccgtccg acaggctcac cccctgcagg
     5881 cgccccgcca gggcaaccaa tcgatagagc gcgccactgt cgagcagggc ccagccgagg
     5941 gctcttgccg cagcgcggct aacggtgcct ttcccggagc cggacggccc atcgatggtc
     6001 atgatcggca cgcggctcat ggcggcgcct cgggggattc ccggacatcg agaccgacgg
     6061 agcgcgccaa atcggcgaaa tcgggaaagg aggtggcgac gttggccaca tcgtgaatgg
     6121 cgatgggccg cgcgcagcgc aggctcgcga tggcaaatga catggctatt cggtgatcgc
     6181 cgaagctgtc gactgcgccg ccgccgaacg ccgcgccgct gccgcggcct tcgatccgca
     6241 tcccgtcggg cagcatctga tgcgccacac ccaggacggc gaagccggcg ctcatcgcgg
     6301 cgattcggtc gctttccttg acccgcagct cctcggcacc cgtcaccagc gtctcgccgg
     6361 ccgcgcatgc ggcggcgacg aaaagcaccg gcaattcgtc gatcgcgagc ggcacgatct
     6421 gcgcgggcac gatcacgccg cgcaatgccg atgcgtgaac cctgagg
//
LOCUS       DASWZT010000272         2606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_50390, whole genome shotgun sequence.
ACCESSION   DASWZT010000272 DASWZT010000000
VERSION     DASWZT010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50390"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..672
                     /locus_tag="VGE92_07275"
     CDS             151..672
                     /locus_tag="VGE92_07275"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEY1313660.1"
                     /translation="MTGADVFILFVLLGSTLIGFLRGFVREAASLVFWILAIWAAWKF
                     GPLVEPHLGGLLADPNVAPWVGRLVVLVLVLLVGWLVGMVLSYFTRSLGLGVMDRVLG
                     VLFGIVRGMVLVGLITIGAELLHLNHEDWWSRSKLVRYGETVGDWLRAMVGEKGDPWA
                     KLERITGVKIKPD"
     gene            687..2192
                     /gene="purF"
                     /locus_tag="VGE92_07280"
     CDS             687..2192
                     /gene="purF"
                     /locus_tag="VGE92_07280"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011060388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY1313661.1"
                     /translation="MCGIVGMVGLSAVNQRIYDALTVLQHRGQDAAGIMTALDGELFM
                     RKDKGLVRDVFQQKHMLKLKGNIGIGHVRYPTAGADSANDAQPFYVNSPYGLCLGHNG
                     NLTNSRELTEVLVREDRRHLNTGSDSEVLLNVLASELQRVGTPRVTPADIFAAANAVY
                     RRCRGGYAVVVMVIGHGILGFRDPNGIRPLVIGKRDTKKGTEWMLASESVALDMLGFD
                     MVRDVAPGEAVFIDEQGRFYAQACVASARHTPCIFEYVYFARPDSIIDNISVYKARLR
                     MGERLAEKIKRERPEHDIDVVIPIPDTSRTSAVQVAQLLGVKYREGFIKNRYIGRTFI
                     MPGQEQRAKSVRSKLNAIDLEFRGKNVLLVDDSIVRGTTSAQIIDMAREAGAKKVYFA
                     SAAPPVRYPNVYGIDMPDVSELVAAGHTDNEVRDIIGADWLIYQDLVDLEHAVRHDNA
                     KIKTFDTSCFSGEYVTGDVTPEYLARLQGERSDEAKQQRREGAGRGLKSVR"
     gene            2223..>2606
                     /locus_tag="VGE92_07285"
     CDS             2223..>2606
                     /locus_tag="VGE92_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313662.1"
                     /translation="MRLLVIAARADYRLLVKKHAEIEWPDVKLVEHRLGDDNPLDAQF
                     AAVGFDAVIIVAAPPTAAAEALAAELAAKPEFAPIVLILLKDTPNPKPSASRGVHRLY
                     GRKIDRDLLVRAIEAASREHKQALSR"
BASE COUNT          472 a          773 c          849 g          512 t
ORIGIN      
        1 gctcaggcgg tcgccaagct gaattcggcg gggcacagcg gcactgtcgc gcccccgggg
       61 ccctgatttg agatttgtga ggttcgtttc accacggcgt gcgcggcgct ccggtagaat
      121 ggatggctgc ggtatacgag ggtcgcggaa ttgaccggag ccgatgtctt tatcctgttc
      181 gttctgttgg gatccactct gatcggattt ctgcgcggat tcgtccgcga agctgcatcg
      241 ctggtttttt ggattcttgc catctgggcg gcctggaaat ttgggcccct cgtcgaaccg
      301 catttgggcg gcttgctcgc agaccccaat gtggcaccgt gggtcgggcg cttagtcgtc
      361 ctggtgctgg tcctgttggt cggctggctc gtcggaatgg tgctgagcta tttcacccgc
      421 agcctcgggc tgggcgtgat ggaccgggtg ttgggcgtgc tgttcgggat cgtgcgcggc
      481 atggtgctcg tcggcttgat cacgatcggc gccgagcttt tgcatttgaa tcacgaagac
      541 tggtggagcc ggtcgaagct ggttcgatac ggtgagacgg tgggcgattg gctgcgggcc
      601 atggtcggcg agaaaggcga cccgtgggcc aagctcgagc gcatcaccgg tgtcaagatc
      661 aagcccgatt aagccggagg tccttcatgt gcggaatcgt cggaatggtg ggtctgagcg
      721 cggtcaatca acgcatctat gacgcgctga ctgtactgca gcatcgtggc caggatgccg
      781 ccggcatcat gaccgccttg gacggcgaac tgttcatgcg caaggataaa ggcctggtgc
      841 gcgacgtgtt tcagcagaaa cacatgctca agctgaaagg caatatcggg atcggccatg
      901 tccgctatcc caccgccggc gccgacagtg caaacgatgc gcaacccttc tacgtcaact
      961 cgccatacgg cctgtgtctc ggccataacg gaaatttgac caattcgcgc gaactcaccg
     1021 aggtgctggt gcgcgaggac cgccgccatc tcaacaccgg ctccgattca gaagtgctgt
     1081 tgaacgtgtt ggcctccgag ctgcagcgcg tcggtacgcc gcgggtcacg ccggccgata
     1141 ttttcgcggc ggccaacgcc gtctatcgcc gctgccgcgg cggttatgcg gtggtggtga
     1201 tggtcatagg ccacggcatc ctggggtttc gcgatcccaa cggcatccgg ccgctcgtga
     1261 tcggcaagcg cgacacgaag aagggaacgg agtggatgct ggcatcggaa agcgtcgcgc
     1321 tcgacatgct cgggttcgac atggtgcgtg acgtggcgcc gggcgaggcg gtgttcatcg
     1381 acgagcaggg gcgtttttat gcgcaagcct gcgtggcgag cgcgcgccac acgccgtgta
     1441 ttttcgaata tgtgtatttc gcgcgacccg actcgatcat cgacaacatc tccgtataca
     1501 aagcgcgctt gcgcatgggc gagcgcctcg ccgagaaaat caagcgcgag cgcccggagc
     1561 atgacatcga tgtggtcatc ccgattcccg acaccagccg cacgagcgcg gtacaggtcg
     1621 cgcagctgct cggcgtcaag tatcgcgagg gcttcatcaa gaatcgctac atcggacgca
     1681 cgttcatcat gcccggccag gagcagcgcg cgaagtcggt gcgcagcaag ttgaatgcga
     1741 tcgacctgga gtttcgcggc aagaatgtgc tcctggtcga cgactcgatc gtgcgcggca
     1801 ccacctccgc gcaaatcatc gacatggcgc gcgaagccgg ggccaaaaag gtctactttg
     1861 cgtccgcagc gccgccggtg cgctatccca acgtctacgg catcgacatg ccggatgttt
     1921 ccgagctggt tgccgccggc cataccgaca acgaagttcg cgacatcatc ggcgccgact
     1981 ggctgatcta ccaggatttg gtggatctgg agcatgcggt gcgccacgac aatgccaaga
     2041 tcaagacttt cgatacctcg tgtttttcgg gtgaatacgt caccggcgat gtgacgccgg
     2101 agtatcttgc gcgcctgcag ggagaacgct ctgacgaagc gaagcagcaa cgtcgcgagg
     2161 gcgccggtcg tggactcaag tccgttcgat agccgccgct gcgcgctgca gcctcaagtg
     2221 ccatgcgcct actggtgatc gcggcgcgag ccgactatcg actgctggtc aagaagcacg
     2281 ctgagatcga gtggccggac gtcaagctcg tcgagcatcg cctgggcgac gacaatccgc
     2341 tcgatgcgca attcgccgcc gtcggctttg acgccgtcat catcgtcgcg gccccaccga
     2401 ctgcggccgc tgaagcactg gcggccgaac tcgcggcgaa gcccgagttc gcgcccatcg
     2461 tactcatctt gctgaaggat acgcctaacc ctaagccgtc tgcgagccgc ggcgtgcacc
     2521 gtttgtacgg ccgcaagatc gatcgcgact tgctggtgag ggccattgag gccgcgtcgc
     2581 gcgaacacaa acaggcgctg tcgcgg
//
LOCUS       DASWZT010000273         5454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_65077, whole genome shotgun sequence.
ACCESSION   DASWZT010000273 DASWZT010000000
VERSION     DASWZT010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5454
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65077"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..3442)
                     /locus_tag="VGE92_07290"
     CDS             complement(35..3442)
                     /locus_tag="VGE92_07290"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY1313663.1"
                     /translation="MKGHTSFKFLAGVLAISVAAAAAPGSVSLQQAQQAADHDVIVIL
                     RDQLENAPPDRESMDERTRAASTAQRPVVNMLQQMRQRSVHSFSLINAFSTKISDAES
                     VQLTAHPAVLAVVPDRTIRAPRIARDAGSAALRAALPLAAAGASSSALCNTLEPEALQ
                     LTHTAYADANTPQAQEVRDGNGRPVTGQGVRVAWIADGMDPNVAGFIHTDGSHVFIDY
                     QNFSGDPAGTATGGEEAFGDASSIAAQDTPNGKPLLFDISSYVVPAHPLPAPCNVRIR
                     GMAPGASLVGLNVFSELGYTTTSNFVQAIEWAVSHDHVDVINESFGGAPFPDTVNDPI
                     SLADAAAVKAGVTVVVSTGDAGTNGTLGSPSTDLDVIAVGATNQWRIYAQTSDGAIPL
                     TPHGGYINNNISAFSSGGFGQFKARTVDVVAPGDSGWALCSTNVAIYTECTDFNNNPT
                     AFQAFGGTSESSPLTAGEAALVIQAYRSTHGGVNPSPALVKQIIMSTATDLGAPAEEQ
                     GAGLINSLSAVNAALSVADSHAKPKARGSQVLSSPSTARITDVPNAGEERSFEITNSG
                     ATALELTPLLETLGAPVATDTLNLTLNPAVDKTFVNSGGNTRTYIKHSFKVPAGVQHL
                     DAAIAFQIDLNSTASPLVLFALLDPSGNQAAYSIPQGSGNGYAHVDVVHPAAGTWTVL
                     VWTRPTGAAGSYAGPVQFVWAAENYVSLGTVSPAHLTLAPGQSETIKAQFSMPSAAGD
                     VGAAIRFNTSPSATSVSLPQIPVSLRTLVATGPTGGSFTGTLTGGNGRAGEGPIQTYE
                     FNVPAGVNNMSLTLQDADSGYVLSGFLIDPNGMELSVETSLDPSGNLAYGLQLFHDNP
                     QPGRWKFVLLQNYFSSGNQTTLPFTARIGFNVAQVSASGLPDSASVQLSASKSTTATI
                     SLINTGSVAQAFFADARLSALGVLSLPAVSNPNCSVSTTLPGTCGQFILPPEVRTVAF
                     VAQAALPINMDAFNNTGFLVGITGSPDIFAKQVAPGTVVASLSEPEIPWGTWDVIPSN
                     VGPYGAAGAPTTPVNMAAFVVAQQFDGTVSADSGDLWADTTFGTATFNPLILASGAAG
                     TITLTIKPSASEVGQVISGFVYIDTFNPNVGTGDEMLRIPYSYTVIP"
     gene            3713..4027
                     /locus_tag="VGE92_07295"
     CDS             3713..4027
                     /locus_tag="VGE92_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313664.1"
                     /translation="MNLLHAAANQDVIGAQFKELFADVDELIRRVADTENPEIRRIRA
                     QVYRASVVAKGALERSSQRADTRRPALPRATLVVSEESPVESPVAMALLLGLGLGLLA
                     SS"
     gene            4116..4538
                     /locus_tag="VGE92_07300"
     CDS             4116..4538
                     /locus_tag="VGE92_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313665.1"
                     /translation="MDTWGNRSGLQPAILGLCGLMSVSSALAAPPGARAGATPHSDSR
                     PALDLRAPAPSLLASSKPDSGFPTSLHQPSFGALEQVQLPELGAQGRVPSRYEEFARR
                     VHREGLPVARLWESRSALVSLGLNQRGKPGLWLIQKTH"
     gene            complement(4558..>5454)
                     /locus_tag="VGE92_07305"
     CDS             complement(4558..>5454)
                     /locus_tag="VGE92_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY1313666.1"
                     /translation="VHLVPAPGRRFGEIAFAHAALAGVAMTGSTATALTINRSLAARD
                     GAIVPLIAETGGLNAMIVDSTALPEQVVDDVLSSAFMSAGQRCSALRLLFLQEDIADH
                     VIDMIAGAMDELIIGDPGDFKTDVGPVITAVAAQGLTQHVARMHKEARIIKACALGEA
                     HAHGSFVAPHLIELKSAAQLTREEFGPLLHVVKFRSADIVKVLQSIRASHYGLTLGVQ
                     TRLESFWRQVFEHTAIGNTYVNRNMIGAVVGVQPFGGNELSGTGPKAGGPHYLSRFAN
                     ERTLTVNTTATGGNAALLNLGN"
BASE COUNT          971 a         1720 c         1748 g         1015 t
ORIGIN      
        1 caacttcccg attcggaagt tggggcgcgc cgctttatgg aatgacggtg tagctgtacg
       61 gaatgcggag catttcatcg ccggtaccga cgttcggatt gaacgtgtcg atatagacga
      121 agccgctgat gacttgcccc acttcgcttg cactcggctt gatcgtcagg gtgatcgtcc
      181 cggctgcgcc ggatgccaag atcaaagggt tgaaggtcgc ggttccgaac gtggtatccg
      241 cccataggtc gccgctatcg gcactgaccg tcccgtcgaa ctgctgcgcc acgacgaacg
      301 cagccatgtt gaccggggtc gtgggagctc ccgccgcacc atagggtccg acgttcgagg
      361 ggatgacatc ccacgtgccc cacgggatct ccggttcact gagcgaggcg acgacggtgc
      421 ccggcgcgac ttgcttggcg aagatgtccg gggaaccggt gattccgacc aggaaacccg
      481 tattgttgaa cgcgtccatg ttgatgggta gcgccgcctg cgcgacgaag gccacggtcc
      541 tcacctcggg gggcaggatg aattggccgc aggtgccggg cagcgttgtc gataccgagc
      601 agtttggatt cgacactgcc ggcaacgaca acaccccgag tgcgctcaag cgcgcatccg
      661 cgaagaacgc ttgagccacc gagcccgtgt tgatcagcga tatcgtcgcc gtcgtcgact
      721 tgctcgccga gagctgcacg ctcgcgctat cgggtaatcc gctggcgctg acctgcgcga
      781 cattgaagcc gattcgcgcg gtgaagggca gcgtcgtctg gttgcccgac gagaagtagt
      841 tctgcagcag cacgaacttc catcgacccg gctgcggatt gtcgtggaac agctgcagcc
      901 cgtacgccaa attgcccgac ggatcgaggc tcgtctcgac gctcagctcc atgccgttcg
      961 gatcaatcag gaagcccgac aggacatagc cgctgtcggc gtcctgcagg gtcagactca
     1021 tattgttcac gccggccggg acattgaatt cgtaagtctg aatcggaccc tctcccgcgc
     1081 ggccattgcc gcccgtcaaa gtcccagtga acgagccgcc ggtcgggccc gtcgccacca
     1141 gcgttcgcaa gctcaccgga atctgcggca gcgaaaccga cgtcgcgctc ggcgaggtgt
     1201 tgaagcggat cgccgcaccg acatcgcccg cagccgacgg catcgagaac tgcgccttga
     1261 tggtttcgct ctgccccggc gcgagagtca gatgcgcggg cgagaccgtg cccaagctca
     1321 cgtagttctc ggcggcccac acgaattgca ccggcccggc ataactgccg gcggcacccg
     1381 tcggccgggt ccaaacgagc accgtccagg ttcccgccgc cggatgcacg acgtcgacgt
     1441 gagcgtagcc gttgcccgag ccctggggaa tggaatacgc ggcttgattg ccggacggat
     1501 cgagcagcgc gaacagcacc agcggcgagg ccgtgctgtt caggtcgatt tggaaggcga
     1561 tcgcggcatc gagatgctgc acacccgccg gtaccttgaa gctgtgtttg atataggtgc
     1621 gggtgttgcc gcccgaattg acgaacgtct tgtctaccgc cggattgagc gtcaggttca
     1681 acgtatccgt tgcgaccggc gccccgagcg tttcgagcag cggcgtcagc tcgagcgcgg
     1741 tggcgcctga attcgtgatt tcgaacgacc gctcttcgcc ggcattcggc acgtcggtga
     1801 tgcgcgccgt gctcggcgaa ctcaggacct gagagccccg cgccttcggc ttggcgtgcg
     1861 aatcggcaac cgacaacgcg gcgttgaccg cagacagcga attgatcagt cccgcgcctt
     1921 gttcctcggc aggcgcacca aggtcggtgg cggtgctcat gatgatctgc ttcaccaggg
     1981 cgggcgaggg attgacgccg ccgtgcgtgc tgcgatacgc ctgaatcacc agcgcagcct
     2041 caccggccgt caagggcgat gattcgctgg tgccgccgaa ggcctggaat gccgtcgggt
     2101 tgttgttgaa atccgtgcac tcggtgtaga tggcgacgtt cgtggagcac agcgcccagc
     2161 cggaatcgcc cggcgcgacg acgtccacgg tgcgcgcttt gaactgccca aagccgccgg
     2221 aactgaacgc ggagatattg ttgttgatgt agccgccgtg cggcgtcagc ggaatggcac
     2281 catcgctggt ctgggcgtag atgcgccatt ggttggtcgc accgaccgca atcacgtcca
     2341 agtccgtgga cgggctgccg agagttccgt tggttccggc atcgccggtg ctgacgacca
     2401 ccgtgactcc ggccttgacc gccgcggcgt cggccagcga aatgggatcg ttgacggtgt
     2461 ccgggaacgg cgcgccgccg aaggattcat tgatcacatc gacgtggtcg tgggacacgg
     2521 cccactcgat cgcctgcacg aagttcgacg tcgtggtgta tcccagttcg gaaaatacat
     2581 tcaacccgac gagcgaggcg cccggcgcca tgccgcggat ccgcacgttg cacggtgccg
     2641 gcaacggatg cgcgggaacc acgtaggagc ttatgtcaaa cagcagcggc ttgccgttcg
     2701 gcgtgtcctg cgcagcgatc gagctcgcat cgccgaaggc ttcctcgccg ccggtcgccg
     2761 tgccggcagg atcaccgctg aaattctggt agtcgatgaa cacatgcgaa ccgtcggtat
     2821 gaatgaagcc ggcgacattg ggatccatgc cgtcggcgat ccacgccacc cgcacacctt
     2881 ggccggtcac ggggcggcca ttgccgtcgc gcacttcctg cgcctgcgga gtgttcgcat
     2941 cggcgtacgc ggtatgcgtg agctgcagcg cttccggctc gagcgtattg cacagcgcgg
     3001 acgaactggc gccggcagcc gccaacggca acgccgctcg gagggccgcg ctcccggcat
     3061 cgcgcgcgat gcgcggcgcg cggatcgtgc ggtccggaac cactgcgagt accgcggggt
     3121 gcgccgtcag ttgcacggac tccgcgtccg aaattttggt cgagaatgcg ttgatgaggc
     3181 tgaaagagtg caccgagcgc tggcgcatct gctgcagcat gttgaccacc ggtcgttgcg
     3241 cggtgctcgc cgcgcgcgtc ctttcgtcca tcgattcgcg atccggcgga gcattctcca
     3301 actgatcgcg caggatcacg atgacatcgt gatcggcggc ttgttgtgcc tgctgtagcg
     3361 acacggaacc gggtgctgct gcggccgcca cgctgatggc gagcacgccg gcaaggaact
     3421 tgaaagaagt gtggcccttc attgagtcgt ctccctagag tttttcacgc gaccgaccgc
     3481 ctcgaggcat tgaaaccccg actcgctcga gggctcggtc gcaaatcacg gtgtttgtgc
     3541 gcgcacttga ccacacccgt gaatgattcg caacagtaaa tgtggtgaag cttttttgaa
     3601 caagtccatg cagcggtctt cgcgccggaa atttcctaca acatgttgcg cacccggact
     3661 gacgcggcgg cgaagctttg gtgggaataa tcactgcaaa cggagtgtcg ccatgaatct
     3721 cctgcatgca gcggcgaacc aagatgtcat tggcgcgcag ttcaaagaat tattcgccga
     3781 cgtcgacgag ttgatccgac gcgtcgccga taccgaaaac cctgaaattc gtagaattcg
     3841 cgcgcaggtg tacagagctt ccgtggtggc gaagggcgcg ctcgagcgtt cctcgcaacg
     3901 tgccgacacg cgcaggcccg cgctgcctcg agccacgctc gtggtcagcg aagaatcgcc
     3961 tgttgaatcg ccggttgcga tggcgttgct actcggcctc ggactcggac tactcgcgtc
     4021 gtcgtgatgt gacataagct gggtctgctt gtcctacacc gagtgctcgc cgccgctgaa
     4081 tgcgccgagg cctcgttccg tgatcgacta tcgccatgga cacgtggggc aatcgcagtg
     4141 gtttgcagcc ggcaatcttg ggattgtgcg gcctcatgtc ggtgagttcg gcccttgccg
     4201 ccccgcccgg cgctcgggcg ggagcgaccc cgcattcgga ctcgcgaccc gcattggatc
     4261 tgcgcgcacc cgcgccctcg cttctcgcct cgtccaaacc tgattctgga tttcccacat
     4321 cgttgcacca gccgtcgttc ggcgcattgg agcaggtgca gcttccggaa ttgggcgcgc
     4381 agggtcgtgt acccagccgc tacgaggaat tcgcgcgccg cgtacatcgc gagggattgc
     4441 cggtggcccg gctgtgggag agccgctcgg cgctcgtcag cttgggcctc aatcagcgcg
     4501 gcaagccggg cctttggctg atacaaaaaa cccattgatc atttcgcgcc gcggcggtca
     4561 gttgccgaga ttgagcaatg ccgcgttgcc gccggttgcc gtggtattga ccgtgagcgt
     4621 gcgctcgttc gcgaagcgcg agagataatg cggaccgccg gccttggggc ccgtccccga
     4681 cagttcgttg ccgccgaacg gttgcacgcc gaccaccgcg ccgatcatgt tgcgattcac
     4741 gtaggtgttg ccgattgcgg tgtgctcgaa gacctggcgc cagaacgact cgagacgggt
     4801 ctgcacgccg agggtgagcc cgtaatggct ggcacggatc gattgcagca ccttgacgat
     4861 gtccgcggat ctaaacttta cgacatgcag tagcggtcca aattcttcac gtgtgagttg
     4921 cgcagcgctc ttcaattcga tgaggtgcgg ggcgacgaaa ctgccgtggg catgcgcctc
     4981 gccgagcgcg cacgccttga tgatgcgcgc ctccttgtgc atgcgcgcca cgtgctgggt
     5041 caaaccctgc gccgcgaccg ccgtgatcac ggggcccaca tcggtcttga aatcgcccgg
     5101 atcgccgatg atcagttcat ccattgcgcc ggcaatcatg tcgatcacgt gatcggcgat
     5161 atcctcctgc agaaacagca ggcgcaaagc cgaacagcgc tggcccgcgc tcatgaacgc
     5221 cgaactcagt acatcgtcga ccacctgttc cggcagcgcc gtcgagtcga cgatcatcgc
     5281 gttcaaaccg ccggtctcgg caatcaacgg cacgatggca ccgtcgcgcg ctgccagact
     5341 gcgattgatg gtgagcgccg tcgcggttga acccgtcatc gcaacccctg ccaaggccgc
     5401 gtgcgcgaac gcaatttcgc cgaagcgccg gcccggcgcg ggcaccaaat gcac
//
LOCUS       DASWZT010000274         4604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_6889, whole genome shotgun sequence.
ACCESSION   DASWZT010000274 DASWZT010000000
VERSION     DASWZT010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6889"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VGE92_07310"
     CDS             complement(<1..893)
                     /locus_tag="VGE92_07310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY1313667.1"
                     /translation="MHIEIAEDASRKISARRDIVLIAALGVVAAVVCAKFNVSEALLA
                     WTRPFERLQLDELPAVLLVIAVSLVWFSSRRYFEATRQLRLRRTVEAQLAAALAENKS
                     LAQQYVDVQEHERKALARDLHDELGQYLNAIKLDAVSIRETLTQHPAAANIARAMIDN
                     IDRVYGVVTGLIRQLRPVGLDDLGVAAALEHCVNDWRSRLAAARIEMSIDADLAALDE
                     TRALVLYRLVQEALTNIARHADASRVQIRIAAAPLGGAQFLDVMVTDNGVGADMSIRR
                     TGLGLVGMRERVAALGGSVSL"
     gene            complement(964..1440)
                     /locus_tag="VGE92_07315"
     CDS             complement(964..1440)
                     /locus_tag="VGE92_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313668.1"
                     /translation="MTSVAAAHSASDAYLTLTAAQASPRTLLHGQWDIALRDLDFVLK
                     LDDNGDGHVTWGETKRHQSDIERYAYAHLRVADDAGSACTIKPTAQMIDEHADGTYAA
                     LFFDVLCAHASKNIDLSYDLFFAIDPSHRGIFVMRDADNVATSVLSPQNASISLAP"
     gene            complement(1476..2732)
                     /locus_tag="VGE92_07320"
     CDS             complement(1476..2732)
                     /locus_tag="VGE92_07320"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1313669.1"
                     /translation="MTAAFSTQHPWASKLVCAAACALASLAAAAATAPLTPAFRAPYL
                     PTADNEILQDVPPAADPAVIDMRRLRSQLDATPQSLAAALQLAGAYIDYSRQIGDAHF
                     AGYAEAVIAPWVSRTPPPAAALVIQATILQYRHQFPEARELLRAALKQDPRNPQGWLT
                     LATLDMVQGSYAAAAKNCAHVAETAGFDLGLACTGNLRAYLGKAHQSLAMLQQAAATA
                     DKGPAAYQAWLQGLIAETAERLGDWPLAETHYLAALKLLPQDNFLLVAYADFLLDRGR
                     PREVLTLLAEHTQSDTAFLRLALAQADLRGAQAARYTWIMAARFEALALRGSDYFGRE
                     QARFALRLQHDPQRSLEMARRNWQVQRAPWDARVLLEAAAAANQPQAAVDVLDFLQET
                     NLEDPIILPLAQKLRAQLNSAAGGRQ"
     gene            2844..3998
                     /locus_tag="VGE92_07325"
     CDS             2844..3998
                     /locus_tag="VGE92_07325"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HEY1313670.1"
                     /translation="MPDFVYVANPGASASRAWFSAAEWARLTALYGFIGILHLLGWGL
                     YLHYAGAYPQLVGLGFVAYMFGLRHAFDADHIAAVDDTVRFMLQKGRQPLAVGFFFSL
                     GHSTVVLALAIAIAVAAASIKMRLPQLQSMGAVIGAGVSGTFLWIIGVLNLLVLLDIL
                     KIWRTAKSGTHSHAHLDVLLQKRGLLNRLFGGRLQKMMNHSWQMYPLGLLFGLGFDTA
                     SEVGLLAMTAGAAAGNLPAPAVLCLPILFAAGMTVMDTTDGVLMTKAYNWAFINPLRK
                     IFYNITTTGLSVAVALLVGSIEILQVFIGLLGVHGRFYDALAALDFGVLGYLIVGMFL
                     VAWALSFAVWRFGRLEQRYSMHAAPHSHMHVHEAGVRHSHDHLHQSESRL"
     gene            3995..>4604
                     /locus_tag="VGE92_07330"
     CDS             3995..>4604
                     /locus_tag="VGE92_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313671.1"
                     /translation="MKGWRRHCAAGLAALLLAAAAVLAATPSRADTVASLLGNFTINQ
                     YCGLKLAQDRIAVHYVVIYGQLPAIRELHNADSNGDGVTSQAERDAYVATLVPDIARQ
                     LELRVDGQALALHATRWTSSLPTEQGGFSLRVDVDLVAALPLAGSGTRSLDFANQNYQ
                     GRMGWHEIVVESAPGVSVFDTNAFNTSLTGGLRDALKSLPTAG"
BASE COUNT          817 a         1420 c         1482 g          885 t
ORIGIN      
        1 ttgagcgaca cggatccgcc gagcgctgca acccgttcgc gcattcccac gagcccaaga
       61 ccggttcgcc ggatcgacat gtcggcgcca acgccgttat cggtcaccat gacgtcgagg
      121 aattgggcgc cgccgagcgg cgcggccgca attctgatct gcacacggct ggcgtcggca
      181 tgacgcgcaa tattggtgag cgcctcctgg acgagacgat acaagaccag cgcacgcgtt
      241 tcgtcgagtg ccgctaaatc ggcatcgatc gacatttcga tcctcgcggc ggcgagccgc
      301 gagcgccagt cattgacaca atgctcgagg gccgcggcaa cgcccaaatc gtcgagaccg
      361 accggccgca actggcgaat gagccccgtc accacgccat agacacgatc gatgttgtca
      421 atcattgcgc gcgcaatatt ggccgcggcc ggatgctgcg tcagcgtctc gcgtatcgac
      481 accgcatcga gcttgatggc gttgagatat tggcccaact catcgtgcag atcccgggcg
      541 agcgccttgc gctcatgttc ctgcacgtcg acatattgct gagccaggct cttattctcg
      601 gccagagctg cggccagttg cgcctccacc gttcggcgca accgaagctg ccgggtcgct
      661 tcaaaatagc gtcgcgagga aaaccacacc aagctgactg caatcaccaa caggacagcc
      721 ggaagttcgt cgagctgcag gcgctcgaag ggccgcgtcc aggcgagcag cgcctcgctg
      781 acgttgaatt tcgcgcagac cacggccgca accacgccga gcgccgcgat caatacgata
      841 tcgcggcgcg cagaaatctt tcgactagca tcctcggcga tttcgatgtg catgcgctgg
      901 cccgccctat tccggcaaca gacgattagt gcattatgcg ctgtccggct gtgccgcgcc
      961 gcttcacgga gcgaggctta tggacgcatt ctgcggcgac agcaccgagg tggctacgtt
     1021 atctgcgtcg cgcatgacga agattccgcg atgcgacgga tcgattgcga aaaacagatc
     1081 gtagctgagg tcgatatttt ttgaggcgtg cgcgcagagc acatcgaaga acaaagcggc
     1141 atacgtgcca tccgcgtgct catcgatcat ctgcgcggtc ggcttgattg tgcaagcgct
     1201 gccggcgtca tcggcgacgc gcaaatgcgc ataggcgtag cgttcgatgt cggactgatg
     1261 acgtttggtc tctccccaag tgacatgccc atcgccgttg tcatcgagct tcaagacgaa
     1321 gtcgaggtcg cgtaaggcaa tgtcccactg accgtgcagc agggtacgcg gtgacgcttg
     1381 ggcggcggtt aacgtcaaat aggcgtcgct ggccgagtgc gcagcagcga cggatgtcaa
     1441 cgcgcacccc gcaagcgaaa gcaacgccgc ccgactcact gccggcctcc ggcggcggaa
     1501 ttcaattgcg cgcggagttt ctgcgcgagc ggcaggatga tcggatcctc gagattcgtc
     1561 tcctgcagga agtcgagcac gtcaaccgcg gcttgcggtt ggttggcggc tgccgccgcc
     1621 tcgagcagca cgcgcgcgtc ccaaggcgcg cgctgcacct gccaattgcg ccgtgccatc
     1681 tccagcgagc gttgtggatc gtgctgcaat cgcaaggcga agcgcgcctg ttcgcggccg
     1741 aaatagtcgc tgccgcgaag cgcgagggcc tcgaaccgtg ccgccatgat ccaggtgtag
     1801 cgggcggcct gcgcaccgcg cagatcggct tgcgccaacg ccagccggag aaacgccgtg
     1861 tccgactgag tgtgctcggc aagcagcgtc agcacttctc tcgggcggcc gcggtcgagc
     1921 aaaaaatccg catacgcgac cagcaggaaa ttgtcttgcg gcagcaattt cagcgcggcg
     1981 aggtagtgtg tttcggcgag cggccagtct cccaggcgct ccgcggtttc ggcgatcagt
     2041 ccttgcagcc aggcttggta ggccgcggga cccttatcgg ccgtggcggc ggcctgttgc
     2101 agcattgcga gactctggtg tgctttgcca agataggcgc gaagattgcc ggtacaggcc
     2161 aaacccaaat cgaacccggc ggtctcggcg acgtgggcgc agttcttcgc ggctgccgca
     2221 tagctgccct gcaccatgtc cagggtcgcc aacgtcagcc agccctgcgg gttgcgcggg
     2281 tcttgcttga gcgccgcccg caacaactcg cgcgcctccg gaaattgatg acggtattgg
     2341 aggatggtgg cttgtatcac cagcgcggcc gcgggcggcg gggtgcgcga cacccagggc
     2401 gcgatcacgg cctcggcgta tcccgcgaag tgcgcgtcgc cgatttgccg gctgtagtcg
     2461 atataagcgc ccgccagttg cagggctgcc gcaaggctct gcggcgtcgc atcgagctgg
     2521 cttcgcagcc gccgcatgtc gataaccgcc ggatccgccg ccggcggcac gtcctgcagg
     2581 atttcgttgt ctgcggtggg gaggtacggc gccctgaagg ctggcgtgag cggggctgtg
     2641 gcggccgccg ccgcaagcga ggcgagtgcg caggcggccg cgcacacgag cttcgatgcc
     2701 caggggtgtt gagttgaaaa agctgcggtc acgtcgggta ttcgcagcgc ggcgcgcaac
     2761 agatgcggcg cggggccttc ggctgcatcc agccgaggcg agttgacgta gactgctaca
     2821 gcgcagtcac gggagtgtgg tcggtgcccg attttgtcta tgttgcaaac cccggcgcga
     2881 gcgcgtcacg cgcctggttc tcggccgccg agtgggctcg gctgaccgct ttgtacggat
     2941 tcatcggcat tttgcatctc ctgggatggg gattgtatct gcattacgcc ggggcctacc
     3001 cgcaactcgt gggcttgggc ttcgtcgcgt atatgtttgg attgcgtcac gccttcgatg
     3061 cagaccacat cgcagcggtg gacgacaccg tgcgcttcat gttgcaaaag ggacgccagc
     3121 cgctcgccgt cgggttcttt ttctcattgg gtcactcgac cgtcgtgctg gcgcttgcga
     3181 ttgcaatcgc cgtggcggca gcgagcataa aaatgcggct gccgcaactg caatccatgg
     3241 gagcggtcat cggcgccggc gtttccggaa cctttctatg gatcatcgga gttctgaatc
     3301 tcctggtcct gctcgacatt ttgaagatct ggcgcacggc gaagtctgga actcacagcc
     3361 acgcccatct tgacgttctg ctacaaaagc gcggcctgct caaccgcctg ttcggcgggc
     3421 gtttgcaaaa aatgatgaat cacagctggc agatgtatcc gctcggtctg ctgttcggct
     3481 taggcttcga cacggcttcg gaagtcgggc tgttggccat gaccgcgggt gccgcggccg
     3541 gcaatctgcc ggcaccggcc gtgctctgcc tgccgatctt gttcgccgcc ggaatgaccg
     3601 taatggacac gacggatggg gtgctgatga cgaaggccta caattgggcc ttcatcaatc
     3661 cgctgcgcaa gatcttctac aacatcacga cgactggatt atcggtcgct gtcgccctgc
     3721 tggtgggcag catcgagata ttgcaagtgt tcatcggact gctcggcgtg cacggccgat
     3781 tctacgatgc cctcgccgct ctcgatttcg gagtgctggg gtatctgatc gtgggaatgt
     3841 ttctggtggc gtgggcgctt tccttcgcag tttggagatt tggtcggctg gaacagcgtt
     3901 attccatgca cgcggcgccc cattcgcata tgcatgtcca cgaggcgggc gtgcggcact
     3961 cgcacgatca cctccatcag agtgaatcca ggttgtgaaa ggttggcgac ggcactgcgc
     4021 ggcaggcctc gcggcgttgc tgctcgcggc agctgccgta ctcgctgcaa cccctagccg
     4081 cgccgacacc gtggcgagct tgttgggcaa tttcacgatc aatcaatatt gcggcctcaa
     4141 attggcgcag gacaggattg ccgttcacta tgtggtcatc tacgggcagc tgccggcaat
     4201 acgcgagctg cataacgccg atagcaacgg cgacggcgtc acctcgcagg ccgagcgcga
     4261 cgcctatgtg gccacgctcg tgccggacat cgcccggcag ctcgagctgc gagtggatgg
     4321 ccaggctctg gcgctgcacg caacccgctg gaccagctcg ctgccgaccg aacaaggcgg
     4381 cttctcgctg cgggtggacg tggatctcgt tgctgcgcta ccgcttgccg gatcgggcac
     4441 gcgaagcctt gacttcgcca accaaaatta tcaggggcgc atggggtggc acgaaatcgt
     4501 ggtcgagtcg gcgcccggcg tgtcggtatt cgataccaat gccttcaaca cgtcgctcac
     4561 cggcgggctg cgagacgcgt tgaagtcgct tcccacggcg ggtc
//
LOCUS       DASWZT010000275         4354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_50455, whole genome shotgun sequence.
ACCESSION   DASWZT010000275 DASWZT010000000
VERSION     DASWZT010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50455"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1442)
                     /gene="glgP"
                     /locus_tag="VGE92_07335"
     CDS             complement(<1..1442)
                     /gene="glgP"
                     /locus_tag="VGE92_07335"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005977 - glycogen metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEY1313672.1"
                     /translation="MRSLYQRHLKFDRLVESSEATARERFEAFALSVRDILAQRWVST
                     ESAYARANPKRVYYLSMEYLIGRSLANNVANLLLDADAHAAVKELGLDWLATLEQEPD
                     AGLGNGGLGRLAACFLDSMATLELPAMGYGLRYEYGMFKQVIQDGWQQEQPDNWLACP
                     DPWEVRQVRDQVEIHLNCSYEVREGTLRAIAGKPSRLIGVPYDRPVVGFGGKTINTLR
                     LWAARAPDYFDFQAFSHGQFVGAVAEALEAESLTRVLYPDDSTSLGQGLRFVQEYFLV
                     ACSLADLVRRFRRSNRDWSLLADKAAVQLNDTHPSFAVPELMRILLDEANLDWDRAWD
                     LTCRTLGYTNHTLLPEALEKWPLEWFELLVPRQLQIIYEINRRLLDAVRSKFPGDEAR
                     IQRISLIEEGPVKHIRMAHLAIVGTHSTNGVAEIHSRLLRTTTVKDLAEMFPERFSNK
                     TNGVTPRRWLLLANPPLAGLIAQAIGSAWMT"
     gene            1578..3023
                     /locus_tag="VGE92_07340"
     CDS             1578..3023
                     /locus_tag="VGE92_07340"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEY1313673.1"
                     /translation="MPTFAAIRTLPHWLADAIVKSLSGIALGLFLAFAVLPLVRWLFP
                     DFQDWSIDRGMRISEMLHPVGSNRTERALGVRGYVFVDVDTGDDQSSQQACDRVSRSF
                     ASPVPTPWDCAPWRPVNRYLLAAIVESLRGRGAKLIVVDVLLDAGGYPLPSQESSALI
                     AALQGAGSPAIAVLPVRPLPDTLPGDELSIEPQAISGLSLAQGLALAAPALPYPGDIL
                     RRYVACYGAHTAQGHESIPSVATRVEAALRGTADARALCQHDPQALPAAMRIAFTLPE
                     VPAEPTTAGASRDSALWNRYHLFLDRCRAIDLDDSDAACSQSSLFREKIAIIGTSSRA
                     RGDRHLTPLGEMSGSMVVLNAIRSRLQENSAVATSLFKQVRHDLAVCGRAACVWFIAF
                     VLLGMIAQRHLGSVAAFSLRLVVVLGALTGVLWVGVKGSFSVAGTPQNLNLMFPLLAF
                     GLEMYTNAARHLEKFIERPINRWIGRVRTQD"
     gene            3027..3941
                     /locus_tag="VGE92_07345"
     CDS             3027..3941
                     /locus_tag="VGE92_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313674.1"
                     /translation="MYSHTGKFVSCLYAALSLSLSSTGAIGAASCNQSPLKISASIEF
                     INPDNATLTVRHAGVADRQARVGDCLIAGDRVIVPATVKLAKVFTNERLIRLQPDTPP
                     YVVTGGMVRIASQAATYLAAVVHALSSEPAPDLPNATASRSFADPPLSALRAIAGLNV
                     ASTQRIPGSVAQIILAWQPLGAAPRCALESAAGGIEASPGAEESWCALRAPDGMTEAV
                     VSIDGANPAASIQWKVVRAADADVPRPAWLDPDSRGALSNADRAVWGLWLYREAGTEW
                     RLAGLSMLVANRSTSWATARATREIIAR"
     gene            3958..>4354
                     /locus_tag="VGE92_07350"
     CDS             3958..>4354
                     /locus_tag="VGE92_07350"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HEY1313675.1"
                     /translation="MYVLRNSLTALRLACALIAPALSRPAVVELFTSEGCSSCPPAEA
                     YLGDLARRADVLALAFHVDYWDDLGWRDRFELPEAVQRQRLYARNLRLASIYTPQIVV
                     DGRGDFVGSDRASIAAALAQSRAGVGVAVA"
BASE COUNT          724 a         1415 c         1374 g          841 t
ORIGIN      
        1 gtcatccacg cgctgccgat tgcctgcgcg atgaggcccg ccagcggcgg atttgcgagc
       61 agcagccagc ggcgcggcgt gacgccgttg gtcttgttgc tgaaacgctc cggaaacatc
      121 tcggccaagt ccttgaccgt ggtggtgcgc agcaacctcg agtggatttc ggcgactccg
      181 ttggtgctat gtgtaccgac aatggccagg tgggccatgc ggatgtgctt taccggcccc
      241 tcttcgatca agctgatccg ctggatgcgc gcctcgtcgc cggggaactt cgagcgtacc
      301 gcgtccaaca ggcggcggtt tatctcgtag atgatttgca attgccgcgg caccaacagc
      361 tcgaaccatt ccagaggcca cttttccaac gcctcgggca acaaggtgtg gttggtgtat
      421 ccgagcgtcc ggcacgtcag atcccaggcg cgatcccaat ctagattcgc ctcgtcgagc
      481 aaaattcgca tcaactccgg gactgcgaag ctcggatgcg tgtcattgag ctgcacggct
      541 gccttgtccg cgagcagact ccagtcgcgg ttgctgcgcc gaaagcgcct gaccagatcg
      601 gcgagcgaac aggccaccaa gaaatactcc tgcacgaagc gcaggccctg gcccaaactg
      661 gtcgagtcat cgggatacag cacgcgggtc aaggattcgg cctcgagcgc ctcggccacg
      721 gccccgacga actggccgtg gctgaaggcc tggaagtcga aataatccgg cgctctggcg
      781 gcccacaacc gtagcgtatt gatggtcttg cccccgaagc ccaccaccgg ccgatcgtaa
      841 ggaactccga tgaggcgcga cggcttgccg gcgatcgcgc gcaaggtgcc ctcgcgcacc
      901 tcgtacgagc agttaagatg gatttccact tggtcgcgca cttgccgcac ttcccacgga
      961 tcagggcagg ccagccaatt gtccggctgc tcctgctgcc atccatcctg gatgacttgt
     1021 ttgaacatgc cgtactcgta gcgcaggccg tagcccatgg cgggcagctc gagcgtcgcc
     1081 atcgaatcga ggaagcacgc ggccagccgc ccgaggccac cattgccgag ccccgcatcc
     1141 ggctcctgct ccaacgtggc cagccagtcg agcccgagtt ctttgaccgc agcgtgcgca
     1201 tcggcatcga gcagcaggtt cgccacgtta ttggccaacg agcgaccgat gagatattcc
     1261 atcgacagat agtagacccg cttggggttc gcccgcgcat aggcgctctc ggtcgatacc
     1321 cagcgctgcg ccaagatatc gcgcaccgac agagcgaacg cctcgaagcg ctcgcgcgcc
     1381 gtggcctccg atgactcgac cagcctatcg aacttgaggt gacgctggta caaggatcgc
     1441 atctcgtccg tgctcaaaat tgccccccgg cgtcgcgtgc gttcaatgtt ccgtcgggaa
     1501 gaataccgcg caaatattgg agaaatgtga acattcaggc gctgatcgtg cggtctgcta
     1561 aagttgaaag tactccaatg ccgacgtttg ccgctatccg caccctaccc cactggcttg
     1621 ccgatgccat cgtaaaaagc ttgtcgggca tcgcactcgg cctgtttctc gcctttgcgg
     1681 tcttgccgct ggtgcgctgg ctgtttcccg acttccaaga ttggagtatc gaccgcggca
     1741 tgcgcatcag cgagatgctg catccggtgg gatcgaaccg caccgagcgc gcgttgggcg
     1801 tgcgcggcta tgtgttcgtc gatgtcgata cgggcgatga ccaatcgagt cagcaggcct
     1861 gcgatcgcgt gtcgcgaagt ttcgcatcgc cggtcccaac gccctgggac tgcgccccct
     1921 ggcgtcccgt caatcgatat ttgttggccg cgatcgtcga atcgctgcgt ggccggggcg
     1981 ccaagctgat cgtggtcgac gtgctgttgg atgccggcgg ctacccgctg ccctcgcagg
     2041 agtcgtcggc gctgatcgcc gcgttgcaag gcgccgggtc gccggcaatc gcggtgctgc
     2101 cggtccggcc gttgcccgat acgcttcccg gcgatgaact gtcgattgag ccgcaggcga
     2161 tatcgggcct gagccttgcg caggggctcg cgctcgctgc gcccgcgctc ccctatcccg
     2221 gcgacatcct gcgccgctat gtcgcttgct acggcgcgca tacggcgcaa ggtcatgaat
     2281 cgattccgag cgtggccact cgcgtcgaag cggcgttgcg cggcaccgcc gacgcgcgcg
     2341 cgctgtgcca gcacgatcca caagcgctgc cggcggcaat gcgcatcgcc ttcacgctgc
     2401 ccgaggtacc cgccgaaccg acgacggccg gtgcgtctcg cgacagcgcg ttgtggaatc
     2461 gttatcacct gtttctcgat cgctgccgcg cgatcgatct ggacgacagc gacgccgcct
     2521 gctcgcaatc gagtctattt cgggaaaaaa ttgccatcat cggaacctcg tcgcgagccc
     2581 gcggcgatcg tcatttgacg ccgctcggcg aaatgtccgg ctccatggtg gtgctcaatg
     2641 ccattcgcag ccgcttgcaa gagaactctg cagttgcgac ttcgctcttc aaacaagtcc
     2701 gtcacgacct ggcggtatgc ggccgggcgg cgtgtgtttg gttcattgcg ttcgtgctgc
     2761 tcggcatgat tgcacagcgg catttggggt ccgtggccgc gttcagcctg cgccttgtcg
     2821 tcgtactcgg cgccctgacc ggcgttctgt gggtcggcgt caaggggtcg ttcagcgttg
     2881 ccggcacgcc gcagaatctg aacttgatgt ttccgttgct ggcgttcggc ttggagatgt
     2941 ataccaatgc tgctcggcac ttggagaaat tcatcgagcg gcccatcaac cggtggatcg
     3001 gcagggttag aacacaggat tgagccatgt attcgcatac cggcaaattc gtctcatgcc
     3061 tttatgccgc cttgagcctg tcgctatcga gcaccggcgc gatcggcgcc gcctcctgca
     3121 accagtcgcc attgaagatc tcggcgtcga tcgaattcat caacccggac aatgcgacgc
     3181 tgacggttcg gcacgcggga gtggcggacc gtcaggcgcg ggtcggcgac tgtctcatcg
     3241 ccggcgatcg ggtgatagtg ccggcgaccg tgaaactcgc caaggtgttc accaatgagc
     3301 gcttgattcg cttgcaaccc gatacgccgc cttacgtggt gaccggcggc atggtgcgca
     3361 tcgccagcca agcggcgact tatctcgctg ccgtggtcca cgcgttgagc agcgagcccg
     3421 cccccgactt gccgaacgcc accgcatcgc gatcgttcgc cgatccgccg ctcagcgctc
     3481 tgcgcgcgat tgccgggttg aatgttgcga gcacgcagcg aattccggga agtgtcgcgc
     3541 aaatcatcct cgcgtggcag ccgctcggcg ccgctccgcg ctgcgcgctc gagagcgccg
     3601 caggcggcat cgaggcatcg ccgggtgcgg aggagtcttg gtgcgccttg cgcgcgcccg
     3661 acggtatgac cgaggccgtc gtctcgatcg atggcgcaaa tcccgcggcc tccatccaat
     3721 ggaaagtcgt tcgggccgcc gacgccgatg tgccgcggcc ggcgtggctc gacccggact
     3781 cgcgcggcgc cctctcgaat gccgaccgcg cggtgtgggg cctgtggctg taccgcgaag
     3841 cgggtaccga atggcgcctt gccggcctgt cgatgctggt cgcgaatcgc tcgacctctt
     3901 gggccacggc gcgagcgacg cgggaaataa tcgcccgcta gctccggtct tctcgccatg
     3961 tacgtattgc gcaattcgtt gacagcgcta cggctcgctt gcgcgctcat cgcgccggca
     4021 ttgtcgcgcc cggcggtggt cgaattattc acctcggaag gctgcagctc gtgcccgccc
     4081 gccgaagcct acctcggcga tttggcgcga cgcgcggacg tgctggcatt ggcattccac
     4141 gtcgactact gggacgattt gggatggcga gaccgtttcg aactgcccga ggcggtgcaa
     4201 cgtcagcgcc tgtacgccag gaacctgcgc ctggcatcga tctatacgcc gcagatcgtg
     4261 gtcgatggac gcggtgattt cgtcggcagc gaccgcgcgt cgatcgcggc agcgctcgct
     4321 caaagccgcg cgggcgttgg ggtcgctgtc gcga
//
LOCUS       DASWZT010000276         2754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_41010, whole genome shotgun sequence.
ACCESSION   DASWZT010000276 DASWZT010000000
VERSION     DASWZT010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41010"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VGE92_07355"
     CDS             <1..304
                     /locus_tag="VGE92_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313676.1"
                     /translation="MGYDLVMTGNYSYTATTQMLADQNPQSIRPSYGLLNLSAGLKSQ
                     TGKFSATIFVDNALNKHYVVDMEDFWSSPWGATNVVVSQPARDTNRYAGIRITAGF"
     gene            400..2448
                     /locus_tag="VGE92_07360"
     CDS             400..2448
                     /locus_tag="VGE92_07360"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEY1313677.1"
                     /translation="MARRQLARGALVVFSWALLVLAGAGARADGDDPPSRVARISFAE
                     GSISFQPAGTQDWVPAPLNRPFTTGDVLWADNTSRAELQLDGSVVRLSNSSELTLVNL
                     DNDVTQLQLSSGTLILHVRRLGDNETYEVDTPNLAFSVLRPGSYRISVDPSGNTTAIA
                     VRSGQGEVSGGGSAYTLQSGDYDSFSGTDQLAAEAQPGPPDADSFDAWSSDRDSRWDH
                     SASARYVSADVVGYQDLDDQGTWRETPEYGAVWYPNSVAAGWAPYQAGHWSYIAPWGY
                     TWVDDQPWGFAPFHYGRWIWGGGAWGWIPCPPRPEHGPYIRPVYAPALVAWVGTGAAI
                     AWFALGPREVFVPRYPVSPNYMHNINVSNTTVNTTVINNVYNTTIINNKTVDITYVNR
                     AAPGAITATTSAAFTSAQPVSRNLVKVDQRELASAPIHPLAPALVPTKQAVLGSSRTA
                     NSKPPAAVLTRSVIARTPPPAAPPTFERRQEALKNNGGKPLSAEQVRQIQRSTAAPRT
                     AAIRVAPPLSPVSLKPVAPRTSAQPPQRAPAANPPPPAAPRMPISHPAPAAIHANELP
                     APPKPASPSVAQSVIDREQLQRQQQMRAQQEQERQRMQQQQELEHQRMAQQQADAARA
                     QELERQHQQQTQELQQRHIQEQQALQATQQEERRSAPPPAPPKPPEQHKPPEPHKPPQ
                     "
     gene            2445..>2754
                     /locus_tag="VGE92_07365"
     CDS             2445..>2754
                     /locus_tag="VGE92_07365"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEY1313678.1"
                     /translation="MSYRAIEAPTPRGLSAAEMRALAPYIAAADLTRAVLHCGTVPWY
                     LPRRFSAIARGHHIYFRPGVYDPHTAAGIALLGHELTHVGQYRMGMTAAHYLCSVIGG
                     Y"
BASE COUNT          542 a          894 c          845 g          473 t
ORIGIN      
        1 aatggggtat gacctggtga tgacaggcaa ttattcctat acggccacga cgcagatgct
       61 cgccgaccag aacccgcagt ccatccgccc gtcctacggc ttgctgaact tgagcgccgg
      121 cttgaaatcg cagaccggca agttctcggc gactattttc gtcgacaacg cgctcaacaa
      181 acactacgtc gtcgatatgg aggatttttg gagttcgccg tggggcgcca ccaacgtggt
      241 cgtgtcgcag ccggcgcgcg acaccaaccg ctatgcgggg attagaatca cggccgggtt
      301 ctagtgatgc aggccgccgg gtgcgcttga gatgtctgcc cgacaagaat agggtaatgg
      361 cagacttaca actaccttta gcggaccggg ggcgattcgg tggcaagacg gcaattggcg
      421 cgtggcgcct tggtggtgtt ttcttgggca ttattggttt tggcgggcgc gggcgcgcgg
      481 gccgacggcg acgatccgcc gtcccgcgtt gcgcgcatct ccttcgccga gggttcgatt
      541 tcctttcaac ccgcgggtac gcaggactgg gtgccggcgc cattgaaccg gccgttcacc
      601 acgggcgatg tcctatgggc ggataacacc agccgcgcgg agctgcagct cgacggatcg
      661 gtcgtgcgtc tgtcgaattc gtctgagctg acgctcgtca atctcgacaa cgacgtcacg
      721 cagctgcagt tgagttcagg gacgttgata ctgcacgtcc gccggctcgg ggacaatgaa
      781 acctacgaag tcgatacgcc caacttggcg ttctcggtac tgcggcccgg ctcgtatcgc
      841 atcagtgtcg atccatccgg caatacgacg gccatcgccg tgcgcagcgg gcaaggcgag
      901 gtgagcggcg gcggctccgc atacacgctc cagtccgggg attacgactc attctcggga
      961 accgatcaat tggcggccga ggcgcaaccg ggtccgcctg atgccgatag cttcgatgcg
     1021 tggagttccg atcgagacag ccgctgggat cactccgcgt cggcgcgtta tgtgtccgcg
     1081 gatgtggtcg ggtaccaaga tctcgatgat caaggcacgt ggcgcgagac gcccgagtac
     1141 ggcgccgtgt ggtacccgaa cagcgtcgcc gcgggttggg cgccgtatca agcgggccac
     1201 tggtcgtaca tcgcgccctg gggctacacc tgggtcgacg atcaaccgtg gggatttgca
     1261 ccttttcatt acggccgttg gatatggggc ggcggcgcat ggggatggat accgtgtccg
     1321 ccacgtccgg aacacggacc ctacatccgc cctgtatacg caccggcctt ggtcgcctgg
     1381 gttggaaccg gcgcagcgat cgcttggttc gccttgggcc cgcgcgaagt cttcgtgccc
     1441 cgctatccgg tcagtccgaa ctacatgcat aacatcaacg tatcgaatac cacggtgaac
     1501 accaccgtga tcaacaatgt gtacaacacg acgatcatca acaacaagac ggtagacatc
     1561 acttatgtga atcgcgccgc tcccggcgcg atcaccgcaa cgacttcggc cgcatttacc
     1621 agtgcccagc cggtatcgag aaacctggtg aaggtcgatc agcgagagct cgccagtgcg
     1681 ccaattcacc cattggctcc ggccctggtg ccgaccaagc aggcggtact gggatcctca
     1741 cgaacggcca acagcaaacc gcccgctgct gtgctgaccc gttccgtaat tgcgcgcacg
     1801 cccccgcccg cggcgccgcc gacattcgag cgccgccaag aggcgctcaa gaacaacggc
     1861 ggtaaacctt tgtcggccga acaagtccga cagattcagc ggtcgaccgc cgcgccacga
     1921 accgcggcga ttcgtgtcgc tcccccgctc tcgcccgtgt ccctcaaacc ggtcgctccg
     1981 cggacgagcg cccagccgcc gcagcgtgcg ccggccgcga atccgccacc gccggccgcg
     2041 ccgcgcatgc cgatcagcca tccggcgcct gccgcgattc atgcgaatga gttgccggcg
     2101 ccgcccaagc ccgcctcgcc gagcgtcgca caatcggtca tcgaccgtga acagctgcag
     2161 cggcaacagc agatgcgtgc tcaacaagaa caggaacggc agcgcatgca gcagcagcag
     2221 gaactcgagc accagagaat ggcacagcag caggcggatg ccgcccgggc gcaggagctc
     2281 gaacggcagc atcagcaaca gacccaggaa ttgcaacaga ggcacattca ggagcagcag
     2341 gcgcttcaag caacgcagca agaagaacga cgctcggccc cgccaccagc gccgccaaaa
     2401 ccgccggagc aacacaagcc gccggagccg cataagccgc cgcagtgagt taccgtgcga
     2461 tcgaggcgcc gactcctcgg gggttgtcgg ctgctgaaat gcgtgcgctg gcaccctaca
     2521 ttgctgcggc ggacctaacg cgcgcggttc ttcactgcgg cacagtgccg tggtacttgc
     2581 caagacgatt cagcgccatc gcgcgcggtc atcacatcta ttttcgaccc ggcgtctacg
     2641 atccacacac ggctgccggc atcgcgctcc tcgggcacga actgactcac gtaggtcaat
     2701 atcgaatggg aatgaccgca gcgcattacc tttgttcggt gatcggcggc tatc
//
LOCUS       DASWZT010000277        23857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_31355, whole genome shotgun sequence.
ACCESSION   DASWZT010000277 DASWZT010000000
VERSION     DASWZT010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 23857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23857
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31355"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VGE92_07370"
     CDS             <1..348
                     /locus_tag="VGE92_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313679.1"
                     /translation="IHTCRTQPPRARAALERFDRISRHGPKQFSWFIHRVSNPTMREL
                     FLDPKNVLRLEEALLSMLAGDVFGRTPIWGRLRLFKALYYILSLANPRRSFRAWRLRA
                     SNIRSEAQHMTSG"
     gene            348..1199
                     /locus_tag="VGE92_07375"
     CDS             348..1199
                     /locus_tag="VGE92_07375"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEY1313680.1"
                     /translation="MTEPNEVRRYRRPRAAAFLAAACAIHSAGASQEPLYEFGLGLGA
                     IVFDDYRGSATAHAYPLPLPYLLYNGTFLKANRDGIRGTLLDQDRFELNLSFDATTPV
                     RSDRARSGMPDLKSTVELGPSFDFHLLKSDAAKVKLDLRMPVRAALTVEALPKIIGWT
                     FTPRLALDIKDPGGFAGWNLGLLTGPLFADRRYHDYFYSVAPQFATPSRPAYAAESGY
                     AGTQFIAALSKRFPRFFVGAYARYDSLSGATFVDSPLVQRRSYMSGGIGFSWIISKSS
                     RMVEVPD"
     gene            1212..1976
                     /locus_tag="VGE92_07380"
     CDS             1212..1976
                     /locus_tag="VGE92_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY1313681.1"
                     /translation="MLRPLKILYEYFALYSSLTLLGFICLTWSVFALPLYFLLPRRAG
                     AAVGRRGIMRGFRIYAWSLSVTGTYRLDLDAIDSLRSGPPLILAPNHPCLIDALLILT
                     RHPNIVCVMKSALMRNIFLGSGSRLARYVRNDSSRQMIKESVAHLREGGVLLLFPEGT
                     RTTRSPINPLVGSVGLIAKHANVPVQTLVIETDSPYLSKGWPLFRRPTLPITYRVRLG
                     KRFEPPADVPAFTAELDRYYRHELEGALQSRWLPNA"
     gene            1969..2724
                     /locus_tag="VGE92_07385"
     CDS             1969..2724
                     /locus_tag="VGE92_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY1313682.1"
                     /translation="MPSDSHLVLIPSYNPGPKVFATVRDALAQWSPVWVVVDGSTDES
                     AQGLQAMAASEPGLRVLVLGENRGKGAAVLFGLLEAHSRGFTHALTMDSDGQHPAQRI
                     RAFMQASQAQPRAMVLGLPVFDDSAPRLRVKGRQISNWWANLETLWAGIGDSLYGFRV
                     YPMAPLIGIMRRQLWMRRFDFDVEAVVRLCWRGVRPINLAAPVRYFRPDEGGVSHFRY
                     WRDNVLLTWMHFRLFLGFVARLPLLLARRRRTP"
     gene            2725..3345
                     /locus_tag="VGE92_07390"
     CDS             2725..3345
                     /locus_tag="VGE92_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313683.1"
                     /translation="MANIFSPLSEMRRAVRITPQMHSFYPGLVITPAETALARELVTE
                     TDLLRLKAIARWHARGLPPDVGWEDLLQEAFARFLGGSRRQPDGVTRVVFLAGVMRSL
                     KAEHWRRVVQETREPPPLPGAQQIDVDALHDPERALTAWQELSTLERLFAEDPLVLQI
                     VSGLAQGLSAAEIRRMHDIPKIEYDSARRRMRRILLREGLTSCGQS"
     gene            3330..3605
                     /locus_tag="VGE92_07395"
     CDS             3330..3605
                     /locus_tag="VGE92_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313684.1"
                     /translation="MRTKLKLADAAAALTRLLEAFERELIESSDEEIMQAAQDLGMNP
                     QMKGSAAFAGLKYRSRLRLADFYDLDLNTSPQPRLTQRPQIKDRDDN"
     gene            complement(3612..5336)
                     /locus_tag="VGE92_07400"
     CDS             complement(3612..5336)
                     /locus_tag="VGE92_07400"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEY1313685.1"
                     /translation="MVIKQISSTRKLLLALGSLLLTLNATAAPTAKSYLIEAHGAAGA
                     AGAAGAVRRVGGRITHELPIIDGVSALLTPAQAARLRKSAELVVIPDMPVSTDALAAN
                     MTGAPLSSVTANVLAAIAPDIYQQTLVGANQLAAEGINGAGVVVAVVDSGILSGNRFL
                     NQDLNGNNRVLAQYDAIHNALVDATHSYVSSVQDLYGHGTHITSLILSSNNVDPVLDG
                     QTQGIAPMAKLISVKAFDNSGAGSYSNVLNGLNWVLTNKSTYGNIRVVNLSFGAPPQS
                     YYWNDPIDQAVMKLWHAGIVVVASAGNFGPSPMSVSVPGNTPYVITVGAMTDNWNPNS
                     SVGWGVTSFSSAGPTFEGFVKPDVVVPGGHLAGLMDKSQYLAATYPQFATGIYDLFVM
                     SGTSQAAGVTTGMVALMLQANPNLTPDQVKCRLTNSASALLNANGKSAYSIFQQGAGI
                     ANAYNAVNSQAANCANQGLNITADIAGTAHYGGPARQNASTGTYYVVGSTGAALGNDG
                     YLWNRTFAKSLGYLSTQGYLWNQGYLWNQGYLWNQGYLWNQGYLWNQSTLAPASTVAA
                     SESWNSQE"
     gene            complement(5539..5787)
                     /locus_tag="VGE92_07405"
     CDS             complement(5539..5787)
                     /locus_tag="VGE92_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313686.1"
                     /translation="MTLFDLGDPHVDEPPQLVGHFQTMLCAHGFEAPVLRGLQINGQS
                     FKRQRRRRTGGEVQLLAHCIHRLRELTDRDVMLMRFVV"
     gene            5853..6155
                     /locus_tag="VGE92_07410"
     CDS             5853..6155
                     /locus_tag="VGE92_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEY1313687.1"
                     /translation="MSDKASLMQQPAPLTDVGLQREIATLFVQALNLEILPQDIDPEA
                     PLYGEGLGLDSIDILEVALIVSKHYGIQLRADSQDNQQIFRSLRHLAEYIAAHRTK"
     gene            6158..6790
                     /locus_tag="VGE92_07415"
     CDS             6158..6790
                     /locus_tag="VGE92_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313688.1"
                     /translation="MGWRRRLQLAAVVFFLIAYAGLSHYSNTVAQTHDLGVGLALGPL
                     LSVALVLLWRWTHFWVALAAAAVAALLLRHYWVVLEKNFALLYLLQEGGFYSIMAASF
                     GQSLLGHRVSLCTQLADKVHGPLTPQELSYTRRVTAAWAIFFLGVTAITVGLFVYAPL
                     RIWSLFANFCAIPLIGLMFVAEYAVRRRVLPQAPRRGILAAVRVYFASSP"
     gene            6800..8449
                     /locus_tag="VGE92_07420"
     CDS             6800..8449
                     /locus_tag="VGE92_07420"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY1313689.1"
                     /translation="MDTIALLSHRTPTAVIAYRAGRAVTAQRFLSDARHLAQRLPAGE
                     HVLNVCADRYRFTVGLAACLISGKVSLLPSTHTPEVIARLASFAPDAFCLTDDERCDI
                     DLPRVHYPELSGDEFAWEIPALPATQIAAIVYTSGSTGVPLPYRKTWGRLARCVQDGA
                     PRLGLLDGRSHVIIGTVPAQHMYGFESTVLLALVSGNSFCAERPFYPADIAAAIASVP
                     RPRALVTTPVHLRTVLAAGIDLPPVDLVVSATAPLTQDLARQVESRFQTRLLEIYGST
                     ETGQIALRRTAESVAWRLWPDVRLKISDQQIFAHGGHVEQVTPMQDVIEITGEGEFLL
                     HGRSADLVNVAGKRSSFGYLNAQLNAIPGVTDGVFFLRDAASGSTAVARLCALVVAPT
                     LSAAAITDQLRQRVDPVFLPRPLIMVERLPRNATGKLPQQELQDLANRHSQAASSMVS
                     PLVILSTHPAFAGHFPTFPVLPGAVLLDEALQAIQHARGIDLTWHIASVKFLGAVRPG
                     DSLSLEHAAPKNGLIRFTIRAPDRVIASGSLSNAAAAGNPA"
     gene            8446..9393
                     /locus_tag="VGE92_07425"
     CDS             8446..9393
                     /locus_tag="VGE92_07425"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313690.1"
                     /translation="MNPKVGNLAEHRPKADWVLHRERGSNLLLRVMAFVSLHLGRSLS
                     RIPLYGIAIYFFLFAPTARRHAQRYLRLALGREPRARDRFRQILSFATTIHDRVYLIN
                     EQFTMFNITLEGEGLVLAQADSGRGALLMGAHMGSFEVMHSLGRRRRGIKVAMAMYEE
                     NARKINAALAAINPRLQADIVSLGRIDAMLNIADRLDQGAFVGVLGDRTLGSEPVQAV
                     TLLGQRAYLPTGPMRAAAILRCPVYFMAGLYRGKNNYHVVFVRLADFSTTSTGSRSAA
                     VRTAIERYAAVLDRYCRSDPYNWFNFHDFWRVADGAPRA"
     gene            9390..10010
                     /locus_tag="VGE92_07430"
     CDS             9390..10010
                     /locus_tag="VGE92_07430"
                     /inference="COORDINATES: protein motif:HMM:NF039988.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA-related protein"
                     /protein_id="HEY1313691.1"
                     /translation="MRRRRYATLLLTLAWLAAVSAAPFASAPPPAPAAPGLPGDLDAL
                     MALLAKRQHGRVEFVEQQFLAVINHPIESSGDMRFDAPDRLEKRTLLPKTETLLLSGG
                     VLTEERGGRTRIIDLHRYPQILPFVESIRATLAGDRAALEHVFHVDFAGSLQRWSLTL
                     VPLDRELLRTVKQVQIDGSRDELLKVEIRQADGDRSLMTLRTPAAP"
     gene            10007..12376
                     /locus_tag="VGE92_07435"
     CDS             10007..12376
                     /locus_tag="VGE92_07435"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY1313692.1"
                     /translation="MKRQRTRVLALWLGCVGIAAIVVARAHYITDLSAFLPARPTPTQ
                     QLLVGQLRDGPASRLILIAFEQGDAAARAKLSAATALRLRRDPQFSSIDNGEPVTAEA
                     DRDFLFQHRYLLSDSVSRERFSASGLHAAIADTIEDLASPAGLMLKSLLPDDPTGEML
                     HIIDQLQRTPAPATRDGVWVSPNGERALMVAQTAAGGSDTDAQQRALAAIRAAFAAAV
                     DTAAAGSVQAVRLRMSGPGVFAVAARAKIERAAVRLSIVSSILVVALLFSVYRSFAAL
                     ALGLAPVASGAVIGIAAVALGFGVVHGITLGFGITLIGESVDYSIYFFVASRARPDAA
                     AAPPWQRTWWPTIRLGMLTSVCGFASLLPSGFPGLAQLGLYSISGLVAAAAVTRYVLP
                     ALLPKGFVIHDVSAVGRRVARLLEPARHLPRLALAALGLALAALALGVLYRHQATLWN
                     HELSYLSPVSAQEQDYDAELRRDLGAADVRDLVIVTAPTLDAALGLAERAARVLDPLV
                     TAHAIGGFDSPANYLPSVATQEARRDSLPGAEQLADNLRQACADLAVRSDRLQPFLHD
                     VDAARRAPLLTPQNLRGTSLRTGFEALVLEQKDRWHALLPLHSVDSRAIDTGRVVAAL
                     RAAQLGAAQVLDLKQESDALYADYLHEAIRLSLAGIAAIVVLLLLALRSPPRVARVLA
                     PLLLAVLTVAAGLVIYRVQLTILHLVGMLLIVAVGSNYALFFDRQSTLHEAGSEALTL
                     ASLVIANASTVIGFGLLSFSQVPVLVSLGTTVAPGAFLALLFAALLSRA"
     gene            12369..13004
                     /locus_tag="VGE92_07440"
     CDS             12369..13004
                     /locus_tag="VGE92_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313693.1"
                     /translation="MRSISAPAAATTAATRMVWLPGAYHAAQDFVSAGFAEAAHARRA
                     ALDLMFVDLELTHVGDRSALLRLRSEIVLPARAAGVSLWLGGISLGGLFALDYAASFP
                     DELDGLCLLAPYLGNRLITREIAQAPGLGAWQPGELAETDEERRIWRYIKNRRADSPL
                     YLGFGAADRFAAAHALLAATLPPDCVDTIAGGHDWPTWLRLWENFLESRFA"
     gene            13001..13855
                     /locus_tag="VGE92_07445"
     CDS             13001..13855
                     /locus_tag="VGE92_07445"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY1313694.1"
                     /translation="MRLETAASEERGVPAWSPSPLLYASAGVHLGAVAALLARPRSWP
                     WALGALAANHVALAAAGLWPRSRWLGPNWTRLPLDGAAAARVAITIDDGPDPQVTPAV
                     LAQLAAHRVHATFFCVGMRVERYADLAREIIRCGHDIENHSQRHRHNFSLLGPGGMRS
                     EISRAQDSISRITGSTPRFFRAPAGLRNPFLDPVLARLQLRLASWTRRGFDTVNANPD
                     TVFRRLAGTLKNGDILLLHDGNAARDRSGKPVILDVLPRLLDAVAAQRLQVATLRSAL
                     PPSYARAQ"
     gene            13852..15045
                     /locus_tag="VGE92_07450"
     CDS             13852..15045
                     /locus_tag="VGE92_07450"
                     /inference="COORDINATES: protein motif:HMM:NF006618.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEY1313695.1"
                     /translation="MRPLLLRHFTATSCIGSGAAATLESLLQQRSGLKGCDFETVDID
                     THIGEVPGVDQTRLPDEMRKFDCRNNRLAELALRQDDFLGAVLEAAERWGRRRVGVFL
                     GTSTAGILQTELAYRERDPVSGALPASFEYGATHNSFSVADYVRQRCRLEGPAVAVSC
                     ACASSAKAFGSARRMIEARLIDAAVVGGVDSLCLTTLYGFHSLQLSATGPCRPFDVAR
                     DGISIGEAAAFALLERAPDKLEPRSVLLLGIGESSDAYHMSAPHPEGLGARRAMQAAL
                     ASAGLDADAIDYINLHGTGTPSNDRAESQAVAGLFGTATPCSSTKGATGHTLGAAGAL
                     EAIISVLAVEHGLLPGGVHTGEIDPLLKIRYLRDNQHAPLQRVLSNSFGFGGTNCSLI
                     FGRAE"
     gene            15042..15839
                     /locus_tag="VGE92_07455"
     CDS             15042..15839
                     /locus_tag="VGE92_07455"
                     /inference="COORDINATES: protein motif:HMM:NF025100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HEY1313696.1"
                     /translation="MSLSAYVSGLGVLGPGLASWPQAAAVLSGEAAYRASASVLPTPT
                     ILPAAERRRVGRTVKLALAVALEASSQAGADRATLATVFTSSGADGHNCHELCQALSL
                     AGREISPTRFANSVHNAAAGYWSIGTGSMAASNVLCAFDASFAAGLLEAMTQVAVDQV
                     DVLLVAYDSEYPQPLHAKRPIPDAFGAALLLTAHRRPSSVATLEATISDLPFGRLTDP
                     ELEGLRRAFPAARALPLLRLLAMRSAGTAILEYLDVSRLQVRVEPCA"
     gene            15830..16276
                     /locus_tag="VGE92_07460"
     CDS             15830..16276
                     /locus_tag="VGE92_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313697.1"
                     /translation="MRLDRHWFEQNIPHQGRMCLLDEVIDWDAQHIRCRASSHRAADN
                     PLRSHGRLGVACGIEYAAQAMAAHGALAGAAPRSELGFLASLREVRLLVPRLDDLESD
                     LICEAALVAADGGSALYEFSLRAAARRLLSGRATVVFDASGRLKPS"
     gene            16273..17004
                     /gene="fabG"
                     /locus_tag="VGE92_07465"
     CDS             16273..17004
                     /gene="fabG"
                     /locus_tag="VGE92_07465"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEY1313698.1"
                     /translation="MNQAMKRALVTGGSGGIGAAICRRLSAAGNFIYVHAHRGTAAAA
                     ATVDEIIAAGGAAAAVSFDLTDAAASRAALETILEAGAVQILVNNAGIHDDAVFPGMS
                     LAQWHRVIDVSVNGFYNATQPLILPMIRTRWGRVISVSSVTALTGNRGQVNYAAAKGA
                     LNAATKALSLEVASRGITVNAVAPGIIATGMGDAAFDAAAIADLVPMKRAGRADEVAS
                     LVGFLASEEAAYVTGQVISINGGMI"
     gene            17006..18346
                     /locus_tag="VGE92_07470"
     CDS             17006..18346
                     /locus_tag="VGE92_07470"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY1313699.1"
                     /translation="MNIPATAAVTGTHKNELLLFYTLLELTIIVLAGRLGGVLAKRCG
                     QAAAVGEIIVGILLGPSLFGLLAPQAFDFVFHSAPPEPLTILSNLGLVLLMFQIGLEF
                     DFTHLNERSNRAAVLRVSIACLVLPFVCGFALGLQIASSSAPATRVDTALFVATAFSI
                     TALPILGRIMIDFDLTRTRLGVIAISSAAVNDVIGWLLLALVTTLTVSDFAAGPFFLR
                     IALVAAFGLVCAKIVRPLLKTAVRKSQPRDGRMSANLLGGLLAVIFSAAMTTYELGIF
                     AIFGGFMIGVILYDERELVAAWRTRIGDFVMVFFLPIFFTYTGLRTAIGGLDSWTDWA
                     WCAAIVATATLAKFGGAYVAARRSGFDHAESSIMGFMMNTRALMELIVINVGLDLGVI
                     SPKTFTMLVIMAIFSTVITTPALRHYLPRIGLAKSSGIGIDGAARGTPRQRAGG"
     gene            complement(18287..19828)
                     /locus_tag="VGE92_07475"
     CDS             complement(18287..19828)
                     /locus_tag="VGE92_07475"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEY1313700.1"
                     /translation="MSDIPAVSYTASESAPAADAAATSAGAGSDSAAPSSKGGDFAVA
                     LDNAGPKPVRKPPTNKGHAGSSGGQMPVPGNVSPPPTLLVPLAAPAPPVGAQPVAAGA
                     TDGAAASATAIAGATAGGEPGADGSSSKAAAANPQVWVAPALGADPADAGVAPTALAD
                     ASAKAAGDLATAPLPASPGAATLAGDAAAKAGATPRNLAANAVDKVPGASQVAAANNA
                     ATSARTGAAGSANPATGSPWMNDAATQAVNTAALSAAATGVVAASKTDAADMDDLATA
                     ATGTAAQAAQVSGGTAVVAPPITAARPASIVSASVLAAASSRATAQTAALISASSEKH
                     YAGGSADALSDTGSDLSAAAAQLSVGSTPSSDATAVPTLKVAAGVDTPEFGQGLSERV
                     SWMLDNNVSSAKLQVNPPQLGPIEVRIALQGDHAQVWLTSHSAVTRDALESSAPKLRE
                     MLGAQGFGQVSVDISQRSFQERSTQPQPYDWTPAAVRAPASAAQSTAGPLPRRTSSGA
                     VDAYA"
     gene            complement(19870..20517)
                     /locus_tag="VGE92_07480"
     CDS             complement(19870..20517)
                     /locus_tag="VGE92_07480"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEY1313701.1"
                     /translation="MTLKTWLLFLVMETALSLSPGPAVFYVVSQGARGALRRSLPAAA
                     GIISANAIYFTLSATSLGAIIAASARFFTIAKWLGAAYLIYLGIKALRSAGSSHAVAL
                     GDGAVAEQGDRRRVYLGALTLQLANPKALLFFLALLPQFIEPLQPVVMQMAILAATSM
                     LPEFCILLGYGWLAHRAVHVSAKFGVSSNMNRWLACIEGAGLLGCATLVLKFNRT"
     gene            20577..22340
                     /locus_tag="VGE92_07485"
     CDS             20577..22340
                     /locus_tag="VGE92_07485"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY1313702.1"
                     /translation="MRNLILALVFTALSSTACAASATAGAGSIESRIAAQNALFEEYY
                     QAELKTHPQRATSYGDYRYNDQLEQISLAAIKELHAADESYLARLQAIDTGSFSEQDL
                     LSHEVLRDTLKQRIENYKFNEFEMPVNQMEGPHLNLADLPLAVPFDSVKQYEDYLSRL
                     HQIPRVFAQSEEVLRQGLKDQLMPVRFLLEKVPAQCQGVIAANPFLLPTQKFPAAVSA
                     EDRQRLSKAITDTVANEVLPAYKEYGEFIATLYAPHGRDALSITALSGGKERYLNDIR
                     SRTTVSDLTPQRIHQIGIAEMQRIQADMLTIARKEGFADLAAFRHSVETNAKYKATSA
                     EQIVDDFRKYVAQMQPKLPDLFIFIPGSPVTVEAMPDFQAANATHYQTGTPDGKRPGR
                     IVVAVSHATERMLVDDEAVAYHEGIPGHHMQLSVAQQLTGLPKFRQHSQNSGYIEGWA
                     LYAEQLGKEIGFYQDPVSDYGRLSSELFRAVRLVVDTGLHSQGWTRDQVVAFMRQSGA
                     VDEPTIQAETDRYISWPAQALAYKLGQLKFRELRDRAQKQLGPKFEIRAFHDEMLSGG
                     VLPLGLLDSRTNSWIQEQKRK"
     gene            22337..23143
                     /gene="panB"
                     /locus_tag="VGE92_07490"
     CDS             22337..23143
                     /gene="panB"
                     /locus_tag="VGE92_07490"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEY1313703.1"
                     /translation="MTTRKPVTLHRLREMHDAGERIATLTCYDAAFARLLDECGVDVL
                     LVGDSLGNVLQGRATTLAVSMQEMAYHTACVARGNRTAFIVADMPFGSYQESAEAAVR
                     NAVLLMQQGAQMVKLEGGGWTVPLVRLLVERSIPVCAHLGFTPQSVHALGGYRVQGRD
                     ETAARVLCRDAEQLAAAGASLLVLEMVPATLARDLTKALSIPTIGIGAGAGCSGQVLV
                     LHDMLGFAGDHPRRFVRNFMEGSASIEAAVRQYVADVKGARFPDDALHSF"
     gene            23153..>23857
                     /gene="panC"
                     /locus_tag="VGE92_07495"
     CDS             23153..>23857
                     /gene="panC"
                     /locus_tag="VGE92_07495"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY1313704.1"
                     /translation="MQVIRSVADLRAALTPKRNIVLVPTMGNLHAGHLSLVRIAQQRG
                     DLVVTSIFVNRLQFAPHEDFATYPRTFDRDCELLTGAGCDVVFAPPDSEVYPEPQGFT
                     LQPPALGGILEGAARPGFFSGVCTVVLKLFNMVEPDTAVFGKKDYQQLLVIQAMVRQL
                     ALPIEIVPAETVRDAQGLALSSRNGYLSEPLQTKASQLQAALAALVAAVKSGRTDWQA
                     LEHQAAQFLGARGWLTD"
BASE COUNT         3867 a         7915 c         7542 g         4533 t
ORIGIN      
        1 atccacacat gccgcacaca accgccgcgc gcgcgcgcgg cgctcgaacg cttcgatcgt
       61 atttcgcgcc acggcccgaa gcaattctcc tggttcatac atcgggtgag taatcccacc
      121 atgcgcgaat tgttcttgga cccgaagaat gtgctgcgcc tcgaggaagc gctgctctcg
      181 atgctggccg gagacgtctt tggccgcacg cccatttggg gacggctgcg cctgttcaag
      241 gcgctgtact acattctgtc gctcgccaat ccccgtcgct ccttccgggc gtggcgcctg
      301 cgcgcgtcca atattcgttc cgaggcgcaa cacatgacca gcggctgatg actgaaccga
      361 atgaggttcg gcgttatcgc cggccgcgcg cggccgcgtt tctcgccgcc gcgtgcgcca
      421 tccacagcgc cggcgcgagc caggaaccac tgtacgaatt cggcctgggg ttgggagcca
      481 tcgtgttcga cgactatcgc ggttccgcta cggcgcacgc ctatccgctg ccgctgccgt
      541 atcttctcta caacggcacg ttcctgaaag cgaatcgcga cggaattcgc ggcacgctgc
      601 tcgatcaaga tcgcttcgaa ctgaatttga gcttcgatgc gaccacgccg gtgcgcagcg
      661 accgtgcgcg cagcggcatg cccgatctta aatcgacggt cgagctgggg ccgtccttcg
      721 actttcatct gctgaagagc gacgctgcca aagtgaagct tgatctgcgc atgccggtac
      781 gcgccgcgct gacggttgag gcgctaccca aaatcatcgg ctggaccttc acgccgcgtt
      841 tggcgctcga catcaaggat ccgggcggat tcgccggctg gaatctcgga ctgctgacgg
      901 gtcccttgtt cgccgaccgc cgctatcacg attatttcta tagcgtggca ccgcagtttg
      961 cgacgccttc ccggcccgcc tatgcggccg agagcggcta cgccggaacc cagttcattg
     1021 ctgcgctatc caagcgcttt ccgaggtttt tcgtcggcgc gtatgcgcgc tacgattcgc
     1081 tgtccggtgc gacgttcgtg gatagtccgt tggtgcagcg caggagctat atgtccggcg
     1141 gcatcggctt ctcctggata atcagcaagt cttcgcgaat ggtcgaagtt cccgattgag
     1201 cgcccgcgcc gatgctgcgt cctctgaaga ttctttacga gtatttcgcg ctctacagtt
     1261 cgttgaccct gcttggattc atctgtttga cgtggtccgt gtttgcgctg ccgctttatt
     1321 ttctcttgcc gcggcgcgcc ggcgccgcgg tcggacgccg cggcatcatg cgcggctttc
     1381 gcatctacgc ctggtctttg tcggtcaccg gcacgtatcg cctggacctc gatgccatcg
     1441 attcgctgcg cagtggaccg ccgctgatct tggccccgaa tcatccctgc ctgatcgatg
     1501 cgctgttgat cctcacgcgt caccccaaca tcgtctgcgt aatgaaatcc gcgctcatgc
     1561 gcaatatttt tctcggctcc ggctcgcgcc tcgcccgcta cgtgcgcaat gactcctcgc
     1621 gccagatgat caaggaatcg gtggcgcatt tgcgcgaggg cggcgtgctg ctgctgtttc
     1681 ccgaaggcac ccgcaccacc cgctcgccca tcaacccttt ggtcggcagc gtcggcctga
     1741 tcgccaagca cgccaatgtg ccggtgcaaa ccctggtgat cgaaaccgac tcgccgtact
     1801 tgagcaaggg ctggccgctg ttccgcaggc cgacgctccc gatcacctac cgggtcaggc
     1861 tcggcaagcg cttcgagcct cccgcggacg ttcccgcctt cacggcggag ctcgaccgct
     1921 actaccggca tgaactcgag ggtgccttgc agtcgcgctg gctgccaaat gcctagcgac
     1981 agccacttgg tgctcatacc gagctacaac ccgggaccca aggtattcgc gaccgtgcgc
     2041 gatgcgctcg cgcaatggtc tcccgtgtgg gtggtggttg acggcagcac cgatgagtcc
     2101 gcgcagggtt tgcaggccat ggccgcctcc gagcccggct tgcgggtcct cgtcttgggc
     2161 gagaaccgcg gcaagggcgc cgcggtgctg ttcggcttgc tggaggcgca ttctcgtggc
     2221 ttcacccacg ctttgacgat ggattccgac ggtcagcacc ccgcgcagcg gattcgcgca
     2281 ttcatgcagg cctcgcaggc gcaaccgcgc gccatggtgt tgggcctgcc ggtgttcgac
     2341 gacagcgcgc ctcgcttgcg ggtcaagggc cgtcaaatct ccaattggtg ggccaatctc
     2401 gagaccttgt gggccggcat cggcgattcc ctgtatggct ttcgcgtcta ccccatggcc
     2461 ccgctgattg gcatcatgcg ccggcaactc tggatgcggc gcttcgattt cgacgtcgag
     2521 gcggtggtgc gcctgtgctg gcgcggcgtg cggcccatca acctggcggc gccggtgcgt
     2581 tattttcgcc ccgacgaagg cggcgtgtcg cactttcgct attggcgcga caatgtgctg
     2641 ctgacctgga tgcactttcg cttgttcctc ggatttgtcg cgcgcctgcc gctgctgctg
     2701 gcgcgccgca ggcgcacgcc ctaaatggca aatattttct cgccgctgtc cgagatgcgc
     2761 cgcgccgtgc gcattactcc tcaaatgcac tctttttacc ccgggctcgt cattacaccg
     2821 gcagaaaccg cgctggcgcg cgagctcgtc accgagacgg atctgctgcg gctcaaagcc
     2881 atcgcgcgct ggcacgcacg cggcttgccg ccggacgtcg gctgggagga cctgctgcag
     2941 gaggcgttcg cgcgcttcct cggcggctct cgccgccagc ccgacggtgt aacccgcgtc
     3001 gtctttctcg ccggcgtcat gcgcagtttg aaagccgaac attggcgccg tgtcgttcag
     3061 gaaactcgcg agccgccgcc gttgcccggc gcgcaacaga tcgatgtcga tgcgctgcac
     3121 gatcccgaac gcgcactgac cgcgtggcag gaattatcga cgctcgaacg cttgtttgcc
     3181 gaagatccgc tggtgctgca gattgtttcc ggtctcgcgc aaggactatc agcggcggag
     3241 attcgacgga tgcatgacat ccccaaaatc gaatacgatt cggcgcgccg acgcatgcgg
     3301 cgtatcttgc ttcgggaggg actgacatca tgcggacaaa gttgaaactg gccgatgccg
     3361 ctgcggcgct gactcggcta ctcgaggcat tcgagcgaga actcatcgaa tcgagcgacg
     3421 aggagatcat gcaagccgcg caggacctgg ggatgaatcc gcagatgaaa ggctcggcgg
     3481 cgtttgccgg gctcaagtac cgcagccgat tgcggctcgc ggatttctac gacctcgatt
     3541 tgaatacctc gccgcagccg cgactcaccc agcggccgca aatcaaggac cgcgatgaca
     3601 actgatccgt ctcactcctg gctgttccaa gattcgctcg cggcaacggt cgaggccggc
     3661 gccagcgtgc tctgattcca gagatagccc tggttccaca aatagccttg attccaaaga
     3721 tacccctggt tccagaggta gccctgattc cacaagtagc cctgcgtcga cagatacccc
     3781 aagctcttcg cgaacgttcg attccacaga tagccgtcgt tgccgagtgc agcgccggtc
     3841 gagccgacca catagtaggt gccggtggag gcgttttgac gtgcagggcc cccatagtga
     3901 gcagtaccgg cgatgtcggc ggtgatgttg agaccctggt tggcgcagtt cgccgcctgg
     3961 ctgttgaccg cgttgtaggc gttcgcgatg cctgcgccct gctgaaagat cgaatacgcc
     4021 gacttgccgt tcgcgttcaa caaggccgag gccgaattgg tcagccgaca tttgacctgg
     4081 tccggcgtca aattggggtt cgcctgcagc atcagcgcga ccatgccggt cgtcactccg
     4141 gccgcctgcg acgttcccga catgacgaac aagtcgtaga tgccggtggc gaattgcggg
     4201 taggtggcgg ccagatactg gctcttgtcc atgagccccg cgaggtggcc gcccggcacg
     4261 accacatcgg gtttgacgaa gccttcgaaa gtcggacctg ccgatgagaa cgaggtcacg
     4321 ccccaaccca ccgacgagtt cgggttccaa ttgtcggtca tcgcgcccac cgtgatcacg
     4381 tacggcgtgt tgccggggac cgagacgctc atcgggctcg gaccgaagtt gccggccgag
     4441 gccaccacga cgatgccggc atgccacagt ttcatgactg cctggtcgat cgggtcattc
     4501 cagtaatacg actgcggcgg cgcgccgaag gacaggttga cgacgcggat gttgccgtag
     4561 gtgctcttat tggtcagcac ccaattcaac ccattgagca cgttcgagta gctgccggcg
     4621 ccgctattat cgaacgcctt gacgctgatc agcttcgcca tcggtgcgat gccctgcgtc
     4681 tgaccgtcga gcaccgggtc gacgttattc gaactcaaga tgagactcgt gatgtgcgtg
     4741 ccgtgtccgt acaagtcctg cacgctgctc acataggaat gagtcgcgtc gaccagcgcg
     4801 ttgtggatgg catcgtattg cgcgagcacg cggttgttgc cgttcagatc ctggttgagg
     4861 aaacgattgc cgctgagtat cccgctgtcg acgacggcga cgacgacgcc ggcgccgttg
     4921 atgccttcgg ccgcgagctg gttggcgccc accaaggtct gttggtaaat gtccggcgcg
     4981 atcgccgcca gcacgttcgc ggtgacactg ctgagcggcg caccggtcat gttcgcagcc
     5041 agcgcatcgg tcgacaccgg catgtccgga atgactacca attcggcgct cttgcgcaag
     5101 cgcgccgctt gcgccggcgt caacagcgcc gaaacgccgt cgatgatcgg caactcgtgc
     5161 gtgatccgcc caccgacgcg gcgcaccgcg ccggccgcgc cggccgcgcc ggccgcgcca
     5221 tgcgcctcga tgaggtagct cttggcggtg ggagccgcag tcgcgttcag cgtcagcagc
     5281 aaactcccga gcgccaacag caatttgcgc gtggatgaga tctgcttgat gaccatgact
     5341 gccgccttgc tttgccgagt gacgccattt catcgcaagc agcgccccgg cggcgcttcc
     5401 gtggtcgaag cgagcgccgg gccaaagcgt gaacgggctc taataatggg tcggttaaac
     5461 tcgcgcgcgg tcgcgaaaat ccggccgcgg acgccaggcg gatctctgcc atgccgatct
     5521 gtaaatctgt atttgagttt atacgacgaa gcgcattaac ataacgtcgc ggtctgtcaa
     5581 ctcgcgcaac cggtggatgc aatgagcaag aagttgaacc tcgccgcccg tccggcgccg
     5641 ccgctggcgc ttaaacgact gaccattgat ttgcaggccg cgcagcaccg gcgcttcaaa
     5701 accgtgtgcg cacagcatgg tctgaaaatg gccgacgagc tgcggcggct catcgacatg
     5761 cggatcgccg agatcgaaca gcgtcaaggc gcatgaatcc gccggcatct atataatgac
     5821 cgcaactgaa aatacattga aagcctaagc ctatgtccga caaagcgtcg ctcatgcaac
     5881 agccggcgcc gctgaccgat gtcggtctgc agcgggaaat cgccaccctg ttcgtgcaag
     5941 ccctgaacct cgaaatcctg cctcaggaca tcgatcccga ggcgccgctg tacggcgagg
     6001 gcttgggcct ggactccatc gacattctgg aggtggcgtt gatcgtctcc aagcactacg
     6061 gcatccagtt gcgcgccgac agccaggata atcagcagat cttccgctcc ttgaggcacc
     6121 tcgcagagta cattgcggca cataggacca agtagcgatg ggttggcggc gtagacttca
     6181 gctggccgcc gtcgtatttt tcctcatcgc ctacgccggg ctctcccact acagcaatac
     6241 cgtcgcgcag acccacgact tgggcgtcgg tctcgctctg ggcccgctgt tatcggtcgc
     6301 cctggtgttg ctgtggcgct ggacccattt ctgggtggcg ctggcggcgg ccgctgtcgc
     6361 cgcgttgctg ctgcgtcatt actgggtggt cttggaaaag aacttcgcgt tgctctacct
     6421 gcttcaagag ggcggcttct acagcatcat ggcggcgagt ttcggccagt cgttgctcgg
     6481 ccatcgggtg tcgctgtgca cgcaattggc ggacaaggtg cacgggccgt tgaccccgca
     6541 ggagctctcc tacacgcgac gcgtgactgc ggcgtgggcg atcttttttc taggggtgac
     6601 cgcgatcacg gtcggacttt tcgtctatgc accgctgcga atctggtcgc tgttcgccaa
     6661 cttctgcgcg ataccgctga tcggactcat gttcgtggct gagtatgccg tgcggcgccg
     6721 cgtactgccg caggcgccgc ggcggggaat attggccgcc gtgcgggtct atttcgcgag
     6781 ttctccctag ccgcgtccga tggacaccat cgcgctactg tcgcatcgca cgcccaccgc
     6841 ggtgattgcc tatcgggcgg gccgcgccgt gaccgcacag cgcttcctct cggacgcccg
     6901 acacctggcg cagaggttgc cggccggcga gcatgtgctc aacgtgtgcg ccgatcgcta
     6961 ccgcttcacg gtcggcctcg ccgcctgcct catcagcggc aaagtcagct tgttgccctc
     7021 gacgcacaca cccgaggtga tcgctcgact ggcgagtttc gcaccggacg ccttctgcct
     7081 gacggatgat gagcgctgcg atatcgacct gcccagggtc cattatcccg aactatccgg
     7141 cgacgagttc gcgtgggaga tccccgcgct tcccgcgacg cagatcgcgg ccatcgttta
     7201 cacctcgggt tccaccggcg tgccgctgcc ctaccggaaa acgtggggac ggcttgcgcg
     7261 ctgcgtgcag gacggtgcgc ctcgcctggg actgctcgac ggcagatcgc atgtcatcat
     7321 cggcaccgtg ccggcacagc atatgtacgg cttcgaatcc accgtcctgc tggccttggt
     7381 cagcggcaat tcgttctgcg cggagcggcc tttctaccct gccgacatcg cggccgcgat
     7441 cgcgtcggta ccgcgcccgc gggccctggt cacgacgccc gtgcacttgc gcaccgtgct
     7501 cgcagcggga atcgatttgc cgccggtcga tctcgtcgtc tccgcgaccg cgcccctcac
     7561 gcaggatttg gcgcgccaag tcgagtcgag gtttcaaacc cggctgctcg aaatatacgg
     7621 ctcgacggaa accgggcaga ttgcgctgcg ccgcaccgcc gagtcggtcg cttggcggtt
     7681 atggccggac gtgcggctga aaatctccga ccagcagatt ttcgcgcacg gcgggcatgt
     7741 cgaacaagtg acccccatgc aggacgtcat cgagatcacc ggcgagggcg aatttctgct
     7801 gcacgggcgc agcgccgact tggtcaatgt ggccggcaaa cgcagttcgt tcgggtattt
     7861 gaacgcgcag ttgaacgcca tccccggagt gaccgacggc gtgttcttcc tgcgcgatgc
     7921 ggccagcggc tccaccgccg tcgcgcgttt gtgcgcgctc gtcgtcgcac cgaccttgag
     7981 cgccgccgcg atcaccgacc agttgcggca acgcgtcgat cccgttttct tgccgcgccc
     8041 gttgatcatg gtcgagcgcc tgccacgcaa cgcgaccggc aaactgccgc agcaggaact
     8101 gcaggatctg gccaaccgtc actcgcaagc tgcgtcgagc atggtgagtc cgctcgtgat
     8161 cttaagcact catccggctt tcgcgggcca ctttcccaca tttcccgtgc tgccgggcgc
     8221 cgtactgctg gatgaggcat tgcaagccat ccaacacgcc cgcggcatcg acctgacgtg
     8281 gcacatagcg tcggtcaaat tcttgggtgc ggtacgtccc ggcgattcat tgagtttgga
     8341 acatgcggct cccaagaatg gcttgatccg cttcaccatc cgcgcgccgg atcgcgtgat
     8401 cgccagcggc agcctgtcga atgcggcggc ggcgggcaac cccgcgtgaa ccccaaggtc
     8461 ggcaacctgg ccgagcatcg gcccaaggcg gactgggtct tgcaccgcga gcgcggcagc
     8521 aacctactgc tgcgcgtcat ggcctttgtc tcgctgcatc tgggaagatc gttaagccgc
     8581 attccgctct atggaatcgc aatttacttc ttcttgttcg ctccgaccgc gcggcgccac
     8641 gcgcagcgct atctgcgcct cgcgctcggc cgcgaaccgc gggcgcgcga ccggtttcgc
     8701 cagatcttga gtttcgccac gaccatccat gatcgggtgt atctgatcaa cgagcaattc
     8761 acgatgttca acatcacgct cgagggcgaa ggcttggtgc tggcgcaagc ggacagcggc
     8821 cgcggtgccc tgctcatggg tgcccacatg ggcagcttcg aggtgatgca cagcctcggc
     8881 cggcgtcgcc gcggcatcaa ggtcgccatg gccatgtacg aggagaatgc gcgcaagatc
     8941 aacgccgcgc tcgccgccat caatccgcgc ctgcaagccg acatcgtctc gctgggtcgg
     9001 atcgatgcga tgctcaatat cgccgatcgc ctggatcagg gtgcctttgt cggcgtgctc
     9061 ggcgatcgca ccttggggag tgaacccgtg caggcggtga cgctcctcgg gcaacgcgcc
     9121 tacctaccca cgggcccgat gcgcgcggcg gctattttgc gctgcccggt gtacttcatg
     9181 gccggtctgt accgcggcaa gaacaactac cacgtggtgt tcgtgcggct ggcggacttc
     9241 tccaccacct ccaccggcag ccgcagcgcc gcagtacgta ccgccatcga gcgctacgcc
     9301 gcagtgctcg atcggtactg ccgcagcgat ccttataatt ggttcaattt ccacgacttc
     9361 tggcgcgtcg cggacggagc gccgcgcgca tgaggcgccg ccgctacgcg acgctgctgc
     9421 tgactctggc gtggcttgca gccgtttcgg ccgcaccctt cgcttcggcc ccgccgcccg
     9481 ctccagccgc ccccggcctg cccggcgatc tcgatgccct catggcgttg ctcgcgaagc
     9541 gtcagcacgg ccgcgtcgaa ttcgtcgagc agcaatttct tgccgtcatc aatcatccga
     9601 tcgagtcctc gggcgacatg cgcttcgacg cgccggatcg cttggagaag cgcacgctgc
     9661 tgcccaagac ggaaacactg ctgctgtccg ggggcgtatt gactgaagag cgcggcggcc
     9721 gcacgcgcat catcgacctg caccgctatc cgcaaatcct gcccttcgtc gagagcatcc
     9781 gggccacgtt ggcgggcgat cgggcagcgc tcgagcatgt tttccatgtg gattttgccg
     9841 gcagtctgca gcgctggagc ttgacactcg tcccattgga ccgcgagttg ctgcgcaccg
     9901 taaagcaggt gcagatcgac ggttcgcgcg acgaattgct gaaggtcgag attcgtcaag
     9961 ccgacggcga tcgatcgctg atgactttgc gcacgcccgc ggcgccatga agcggcagcg
    10021 cacgcgggtg ctggcgctct ggctcggctg cgtcggcatc gccgccatcg tcgtggcgcg
    10081 cgcgcattac atcacggact tgtcggcgtt tttacccgca agacccaccc caacccaaca
    10141 attgctggtc gggcagctgc gcgatggccc ggcatcgcgg ctgattctga tcgcattcga
    10201 acagggcgat gccgcggcgc gcgccaagct gtccgctgcg acggcgctta ggctgcgccg
    10261 cgacccgcag ttctcgagca tcgacaacgg cgagccggtg accgcggagg ccgatcgcga
    10321 ttttctgttc caacaccgct atctactcag tgactcggtg tcccgagaac gtttttcagc
    10381 ctcgggactg cacgccgcga tcgccgacac gatcgaggat ctggcctcac ccgccggact
    10441 catgctgaag tccttgctgc cggacgatcc cacgggcgag atgctgcaca tcatcgatca
    10501 actgcagcgc acgcccgctc ccgccacgcg ggatggcgtc tgggtttcgc ccaacggcga
    10561 gcgggcgctg atggtcgcgc aaactgcggc cggcggatcc gataccgacg cgcagcagcg
    10621 tgccctcgcc gccattcgcg cggcgttcgc cgccgccgtg gacacagcgg cggccggctc
    10681 cgtgcaggca gtgcgcctgc ggatgagcgg acccggcgtg tttgccgtcg cggcgcgcgc
    10741 caagatcgag cgcgccgcag tgcggctgtc gatcgtcagc agcatactgg tcgtcgccct
    10801 cctattctcc gtgtaccgct cgttcgcggc gctcgcgctc ggactggcgc cggtggcgag
    10861 cggcgcggtc atcggcatcg ctgcggtcgc gttgggcttt ggcgtggtcc acggcatcac
    10921 cttgggcttc ggcatcacct tgatcggcga gtcggtggac tactcgatct attttttcgt
    10981 ggcctcccgc gcccggcctg acgctgcggc cgcgccgccc tggcaacgaa cctggtggcc
    11041 gacgatccgc ttggggatgc tcacctccgt ctgcggcttc gcgtcgctgc tgccttctgg
    11101 ttttcccgga ctcgcacagc tcggcctgta ttcgatcagc ggcttggttg cggcggccgc
    11161 ggtcacccgc tatgtattgc cggccttgct gccgaagggg ttcgtcatcc acgacgtctc
    11221 tgccgtgggg cgccgagtcg cgcgcctgct cgagccggcg cggcatctgc ctcggctcgc
    11281 actcgctgcg ctcggcctgg cgctggcggc gttggcgctc ggcgtgctgt accggcatca
    11341 agccacctta tggaatcatg aactgtccta tttaagtccc gtctcggcgc aggagcaaga
    11401 ctacgatgcc gagctgcgcc gcgacttggg ggccgccgac gttcgagatc tcgtgatcgt
    11461 gacggcgccg acgctggacg cggcgctcgg cttggccgag cgcgcggcgc gggtgctcga
    11521 cccgttggta accgcgcatg cgatcggcgg attcgacagc ccggcgaact atctccccag
    11581 tgtcgcgact caagaggcgc gtcgcgacag cctgccgggc gcggaacaac ttgccgacaa
    11641 cctgcggcaa gcctgcgccg atctcgccgt tcgcagcgat cggctgcagc ccttcttgca
    11701 cgatgtcgac gcggcgcggc gcgcgcctct gctcacgccg cagaatctac gaggcacctc
    11761 gttgcgcacc ggcttcgagg cgctcgtcct cgagcagaag gatcgctggc atgcgctgct
    11821 gccgctgcac agcgtcgatt cacgcgcgat cgataccggg cgtgtggtgg ccgccttgcg
    11881 ggcggcgcaa ctcggcgctg cccaagttct cgatcttaaa caagagtccg acgcgctgta
    11941 tgcggactac ttgcacgagg cgatccgctt gtcgctggcg gggatcgccg cgatcgtcgt
    12001 cctgttgttg ctcgcgctgc gctcgccgcc gcgcgtcgcg cgcgtattgg caccgctgct
    12061 gctcgccgta ttgacggtgg ccgccggtct cgtgatttat cgggtacaac tgacgatact
    12121 gcacctggtg gggatgctgc tcatcgtggc cgtcggctcg aactacgccc tgttttttga
    12181 ccgccagagc accttgcacg aagccggcag cgaagcgctg acgctggcat cgctggtcat
    12241 cgccaatgcc agcacggtga tcggcttcgg tcttctgtcc ttctctcaag taccggtgct
    12301 ggtgtcgttg ggcacgaccg tggcaccggg cgcttttctt gcgctgctgt tcgccgcgct
    12361 gctgtcgcgt gcgtagtatt tcagcgcccg ccgccgcgac cacggcagcc acgcgcatgg
    12421 tgtggctgcc cggggcttat catgctgcgc aagatttcgt gagcgccggt tttgcagagg
    12481 cggcccatgc gcggcgcgcg gcgctcgatt tgatgtttgt cgacctcgaa ttgacgcacg
    12541 tcggcgatcg cagcgcgctt ctgcgcctgc gatccgagat cgtactgccg gcgcgcgccg
    12601 ccggcgtttc gctgtggttg ggcggcatct cgctcggcgg tctgtttgcg ctcgattacg
    12661 cggcgtcgtt cccggatgaa cttgacggcc tgtgtctgct cgcaccctat ctcggcaatc
    12721 ggctcataac ccgcgaaatc gcgcaggcac cgggcctcgg cgcatggcaa ccgggcgaac
    12781 ttgcggaaac ggacgaagag cggcgaatat ggcgctatat caagaaccgt cgtgccgatt
    12841 cgccgttgta tctcggattc ggagcggccg accgtttcgc cgcggcgcac gcgctgctgg
    12901 cggcgacgct gccgccggat tgtgtcgaca cgatcgcggg cggtcacgat tggcccacct
    12961 ggttgaggct atgggagaac ttcttggagt cgcgcttcgc atgagactcg agactgcggc
    13021 atccgaggaa cgcggcgtac ctgcttggtc gccatcgccg ctgctatatg cgtcggccgg
    13081 cgtgcatctc ggtgccgtcg cggcgctgtt ggcgcggccg cgcagctggc cctgggcgct
    13141 cggcgccctg gcagcgaatc atgtggcgtt ggcggcggcc ggtctttggc cgcgcagccg
    13201 ctggctcggt ccgaactgga cccgtctgcc gctcgacggc gccgcggcag cgcgcgtggc
    13261 gatcaccatc gacgatgggc ccgacccgca agtgacgccg gcggttcttg cgcaactcgc
    13321 cgcgcatcgt gtgcacgcca cctttttttg cgtgggcatg cgggtcgaac gctacgcgga
    13381 tctggcgcgt gaaatcatcc gctgcggcca cgacatcgaa aatcacagcc agcggcatcg
    13441 gcacaacttt tcgctgctgg gtccgggcgg catgcgttcg gaaatttcgc gcgcgcagga
    13501 cagcatttcg cgcatcaccg gcagcacgcc gcgcttcttt cgggcgcctg cggggctgcg
    13561 taatccgttt ctcgatccgg tgctcgcgcg cttgcagctg cggctcgcca gctggacacg
    13621 ccgcggcttc gacaccgtca atgcgaaccc cgacacggtg ttccggcgct tggcgggtac
    13681 gctgaaaaac ggcgacattt tgttgctgca cgatggcaac gcagcgcgcg accgcagcgg
    13741 taaaccggtg atcctcgacg tattgccgcg gctgctcgat gccgtcgccg cgcaacggct
    13801 gcaggtcgct accttgcgct ccgcgttgcc gccctcgtac gctagggcgc aatgagaccg
    13861 ctgctgctcc gacacttcac cgccacaagc tgcatcggca gcggcgcggc cgcaaccctg
    13921 gagagcctgc tgcagcagcg tagcggcctg aaaggctgcg atttcgagac cgtcgacatc
    13981 gacacgcaca tcggcgaggt gcccggagtc gatcagacgc ggttgccgga cgaaatgcgc
    14041 aaattcgatt gccgcaacaa ccgcctggcc gaattggcgc tgcggcagga cgattttctc
    14101 ggcgccgtac tcgaggcggc cgagcgctgg ggccggcggc gcgtcggggt tttcctcgga
    14161 accagcacgg cgggcattct gcagaccgaa ctcgcctatc gagagcgcga tcccgtgagc
    14221 ggcgcgctgc cggcgagctt cgagtatggc gcgacccaca actcgttctc cgtggccgac
    14281 tacgtgcgcc aacgctgccg cttggagggg cccgccgtgg cagtctcctg cgcctgcgcc
    14341 tcgagtgcca aggccttcgg ctccgcgcgg cgcatgatcg aggcgcgact catcgatgcg
    14401 gcggtggtgg gcggagtgga ttcattgtgc ctgacgacac tgtatggatt tcactccctg
    14461 caactgtccg cgaccgggcc gtgccgtccc ttcgacgtcg cgcgcgatgg catctccatc
    14521 ggagaggctg cggcatttgc gcttctcgag cgcgcgccgg acaaactcga gccgcgctcc
    14581 gttctgctgc tcgggatcgg cgaatccagc gatgcctatc acatgtcggc gccgcatccc
    14641 gaaggtctcg gtgcgcgccg tgcaatgcaa gccgccctgg cgagcgccgg actcgacgcc
    14701 gatgccatcg attatatcaa tctgcatggc accggcacgc caagcaacga tcgtgccgaa
    14761 agccaggcgg tcgccggctt attcggcacc gcgacgccgt gcagctcgac caagggtgcc
    14821 accggccata cgctgggcgc ggccggggcg ctcgaggcca tcatcagtgt gctcgccgtc
    14881 gagcacggcc tgctgccggg cggcgtacac accggcgaaa tcgatccgct gctcaagatc
    14941 cgctatctgc gggacaatca gcacgcgccg ctgcagcgcg tgctctccaa ctcatttggg
    15001 tttggcggca ccaactgcag cctgattttt ggtcgtgcag agtgagtttg tctgcctacg
    15061 tcagcggcct cggcgtgttg gggccgggcc tcgcgagttg gccgcaggcg gcggccgtgc
    15121 tgtcgggcga agcggcctat cgggcgtcgg cctcggtgct gccgacgccc acgatcctcc
    15181 ctgccgcgga gcggcggcgc gtgggccgca ccgtcaaatt agccctcgcg gtggcgctcg
    15241 aagcgagctc gcaagccggc gcggatcgcg ccacactcgc cactgtattc acatcctccg
    15301 gagcggacgg ccacaattgt catgaactct gccaggcgct gtcgctcgcg gggcgcgaga
    15361 tttcaccgac gcgctttgcc aactcggtgc acaacgcggc ggcgggctat tggagcatcg
    15421 gcaccggctc gatggcggca tccaacgtgc tgtgcgcgtt cgacgcaagc ttcgccgccg
    15481 gcctgctcga agccatgacc caggttgccg tcgatcaagt ggacgtgttg ttggtggcct
    15541 acgacagcga gtatccgcag ccgctgcacg ccaaacgccc gatccccgat gcatttggtg
    15601 ccgcgttgct gctcactgcg cacagacgtc cctcctcggt ggcgacactc gaggccacga
    15661 tcagcgacct gccgttcggg cggctgaccg atcccgagct tgagggcctg cgccgcgcct
    15721 tcccggcggc acgcgcgctg ccgttgctgc gtttgctggc gatgagatcc gccggcacgg
    15781 ccatcctcga gtatctcgac gtgtcccgcc tgcaagtgcg ggtcgagcca tgcgcctaga
    15841 ccgtcactgg ttcgagcaga acattcctca ccagggccgc atgtgcttgc tcgatgaggt
    15901 catcgattgg gacgcgcagc acatccggtg tcgcgcgagc tcgcatcgcg ccgccgataa
    15961 tccgctgcgc tcgcacggac gcttgggtgt agcctgcgga atcgaatacg ccgcgcaggc
    16021 catggccgcg catggcgcgc tcgccggcgc cgcgcctcga tccgagcttg gatttctcgc
    16081 gagcctgcgc gaggtgcggc tgctcgtccc gcggctcgat gacctggaat cggatctcat
    16141 ctgcgaggcg gcgctcgttg ccgcggacgg cggcagcgca ctctatgaat tctcgctgcg
    16201 cgccgctgca cggcgcttgt tgagcggccg cgcgacggtg gttttcgacg cgagcgggcg
    16261 gctcaagccg tcatgaatca ggcgatgaag cgcgccttgg tcacgggcgg cagcggcggc
    16321 atcggcgcgg ccatctgccg gcgtttgagc gctgccggca atttcatcta cgtccacgcg
    16381 catcgcggca ccgcggccgc ggcagccacg gtcgacgaga tcatcgcagc cggcggcgcc
    16441 gccgcggcgg tgagtttcga tttgacggac gccgccgcat cccgcgcggc gctcgagaca
    16501 atactcgaag cgggagccgt gcaaatcctg gtgaacaacg ccggcatcca tgacgacgcc
    16561 gtgttcccgg gaatgagtct cgcgcaatgg catcgcgtca tcgacgtgtc ggtcaacggc
    16621 ttctataacg cgacgcagcc cttgatcctg ccgatgattc gcacgcgctg gggccgcgtc
    16681 atcagcgtct cgtcggtcac ggcactcacc ggcaatcgcg gccaggtgaa ttatgcggcg
    16741 gccaagggcg cgctcaacgc cgccaccaag gcgttgagcc tggaagtggc gagccgcggg
    16801 atcacggtca acgcagtcgc tcccggtatc atcgccacgg gcatgggcga tgccgccttc
    16861 gatgccgccg ccatcgccga cttggtgccg atgaagcgcg ccggacgcgc cgacgaagtc
    16921 gcgagtctgg tggggtttct cgcctcggag gaggccgcgt atgtcaccgg ccaggtcatt
    16981 tccatcaacg gcggcatgat ttagcgtgaa tattccagcc acggcggcgg tgaccggcac
    17041 gcacaagaac gaacttctgc tgttctacac gctgctcgaa ctcaccatca tcgttcttgc
    17101 cggccggctc ggcggtgtgc tcgccaagcg ttgcgggcaa gcggcggccg taggtgaaat
    17161 catcgtcggc atcttgctgg gcccgtcgct cttcgggctg ctcgcgccgc aggccttcga
    17221 ttttgtgttt cattccgcgc cgccggaacc gttgacgatc ctatcgaact tggggctcgt
    17281 gctgctcatg ttccaaatcg gactcgagtt cgacttcacg cacctcaacg agcggtcgaa
    17341 tcgcgccgcc gtgctgcgcg tgtcgatcgc ctgtctggtg ctgccgttcg tctgcggctt
    17401 cgcgctcggc ttgcaaatcg catcgagttc cgcgccggca acccgcgtcg acaccgcgct
    17461 gtttgtcgcg accgcgtttt ccatcacggc actgccgatc ctgggccgga tcatgatcga
    17521 cttcgacttg acccgtactc ggctcggcgt catcgccatc agttccgcgg ccgtcaacga
    17581 cgtcatcggc tggctgttgc tcgcgctcgt caccacgctc acggtcagcg actttgccgc
    17641 cggcccattc ttcctgcgca tcgccttggt ggcggccttt ggcctggtgt gcgcgaaaat
    17701 tgtccgcccg ctgctcaaga cggcggtccg aaaatcgcaa ccgcgcgacg gcaggatgtc
    17761 ggcgaacttg ttgggcggac tgttggcggt gatcttcagc gctgcgatga cgacttatga
    17821 gctcggcatt ttcgcgattt ttggcggctt catgattggc gtaattctct acgatgaacg
    17881 cgaattggtc gcagcgtggc gcacgcgcat cggcgacttc gtcatggtgt tttttctgcc
    17941 tatttttttc acttatacgg gactgcgcac cgcgatcggc ggactcgaca gctggacgga
    18001 ctgggcctgg tgtgccgcca ttgtcgccac cgcgacgctc gcgaaattcg gcggcgctta
    18061 cgttgcggcg cgccgctccg gtttcgacca cgccgagtcc agcatcatgg gattcatgat
    18121 gaacacccgc gccttgatgg agctcatcgt gatcaacgtc ggtctcgatc tcggcgtgat
    18181 atcgcccaag accttcacca tgctggtcat catggcaatc ttcagcaccg tgatcacgac
    18241 gccggcgctg cgccactacc tgccgcgaat cgggctggcg aaatcctcag gcataggcat
    18301 cgacggcgcc gctcgaggta cgccgcggca gagggccggc ggttgattgc gcggcgctcg
    18361 ccggggcgcg gacggccgcg ggcgtccagt cgtaaggctg cggttgggtc gaacgctcct
    18421 ggaaggagcg ctgcgagata tcgacgctga cttggccaaa gccttgcgcg cccagcatct
    18481 cgcgcagttt cggcgcgctc gactcgagtg catcgcgcgt cacggcacta tgactcgtga
    18541 gccacacctg cgcatggtca ccctgcagcg cgattcgcac ttcgatgggc ccgagctgcg
    18601 gcggatttac ctgcaacttc gcgctgctca cattgttgtc gagcatccac gagacgcgct
    18661 cgctcaagcc ttgtccgaac tccggggtgt cgaccccggc agcgactttc agagtgggca
    18721 ccgccgttgc atcgctgctc ggcgtcgagc cgacgctcaa ttgcgcggcg gccgcactca
    18781 agtcgctccc ggtatcggat agagcatcgg cgctgccgcc ggcgtaatgt ttttccgatg
    18841 atgcgctgat gagcgcggcc gtctgtgcag tcgcgcggct gctcgcggcg gctagcacgg
    18901 atgcggatac gatgctcgcg ggccgggccg ctgtaatcgg cggcgccacc acggcagtgc
    18961 cgcccgagac ttgggcggcc tgggccgcgg tgcctgtcgc cgccgttgcc agatcgtcca
    19021 tatccgcagc gtctgtcttg ctcgcggcca ccactccggt tgccgccgcc gagagggccg
    19081 cagtgttgac cgcctgcgtc gccgcgtcgt tcatccaagg ggaaccggtc gccgggttcg
    19141 cgctgccggc cgcgccggtg cgcgccgaag tcgccgcatt gttggcggcc gcgacctgtg
    19201 aggcgccggg gaccttatcg accgcgttcg ccgcgaggtt gcgcggcgtc gcgccggcct
    19261 tcgctgccgc gtcacctgca agcgtcgccg cgccgggaga tgccggcagc ggcgcggttg
    19321 cgagatcgcc ggccgccttg gcgcttgcgt cggcaagcgc tgtcggtgcc acgccggcgt
    19381 ctgcgggatc cgcgcccagt gcgggggcaa cccatacctg aggattcgcc gcggctgctt
    19441 tcgatgatga tccgtccgcg cccggctcgc cgcccgcggt cgcgcccgca attgcggtcg
    19501 ccgacgcggc agcgccgtcc gtcgcgccgg cggcgaccgg ctgggcccca accggcggcg
    19561 caggcgctgc cagcggcacg agtagagtcg ggggcggcga aacattgccg ggcactggca
    19621 tttggccgcc gcttgacccg gcatggccct tgttcgtcgg cggtttacgc accggcttgg
    19681 gccctgcatt gtcgagcgcg acggcgaagt cgccgccctt gctgctgggg gcggcggaat
    19741 cgctgccggc gccggcgctg gtcgcggccg catcggcggc cggggctgat tcgctggcgg
    19801 tgtacgaaac ggcgggtatg tcactcatac ccgggaattt gcaagccacg tgccaagcgc
    19861 ggccgaaact caggtccgat tgaacttcaa gaccagcgtt gcgcacccga gtaagcccgc
    19921 tccctcgatg cacgccagcc agcggttcat gttgctcgac accccgaact tggcagagac
    19981 gtgcacggcg cgatgcgcta gccagccgta acccaagaga atgcagaatt ccggcaacat
    20041 cgacgttgcc gcgaggatcg ccatttgcat gaccaccggc tgcagcggct cgatgaattg
    20101 cggcaacagc gccaggaaga acaacagcgc cttgggattc gcgagctgca gcgtaagggc
    20161 tcccaaatag acgcggcgcc gatcgccctg ctcggccaca gcgccgtcgc ctaaagccac
    20221 tgcatgactc gaaccggccg agcgcagcgc cttgatgccg agataaatga ggtatgccgc
    20281 gccgagccac ttggcaatcg tgaagaagcg cgcgctcgcg gcgatgatgg cgccaaggct
    20341 ggtcgccgac agggtgaaat agatagcatt ggcgctgatg atgcccgccg cggccggcag
    20401 cgatctgcgc agcgcgccgc gcgcaccctg cgagacgacg taaaacaccg ccggacccgg
    20461 cgacagcgat aaggctgttt ccatcaccaa gaaaagcagc caggttttaa gcgtcatagg
    20521 cacccgcgca atgtttccgg cgctagctta ccagtctgta tgctaacgtt tagctcatgc
    20581 gtaacctgat actcgccctc gtctttaccg ccctgagttc cactgcctgc gccgcctccg
    20641 cgaccgcggg cgccggttcg atcgaatcgc ggatcgcggc tcaaaacgcc ctcttcgagg
    20701 agtactacca agcggagctc aagacccatc cgcaacgcgc gacctcctat ggcgactacc
    20761 gttacaacga ccagctcgag caaatttccc tcgcggcgat caaggaattg cacgccgccg
    20821 acgagagtta tcttgcgcgt cttcaagcca tcgacaccgg cagcttctcc gagcaggacc
    20881 tgctgtcgca cgaggttttg cgcgacacgc tcaagcagcg aatcgaaaat tacaaattca
    20941 atgagttcga gatgccggtc aaccaaatgg agggtcccca tctgaacctg gccgacttgc
    21001 ccctcgcggt gcccttcgat tcggtcaagc agtacgagga ttacctctcc cgtctgcatc
    21061 agatcccgcg cgtgttcgcc cagtccgaag aggtgctgcg ccagggcctg aaggaccagc
    21121 tcatgccggt acgctttctg ctcgagaagg ttcccgcgca atgccagggt gtcatcgcgg
    21181 ccaatccgtt tctgctcccg acccagaaat ttccagcggc cgtctccgcg gaagatcggc
    21241 aacggctgag caaggccatc accgacacgg tcgcgaacga agtcttacca gcctacaagg
    21301 aatatggcga gttcatcgcg acgctgtacg ccccgcacgg ccgcgacgcg ctcagtatca
    21361 ctgcgctgag cggaggcaaa gagcgctacc tcaacgacat ccgcagccgc accaccgtga
    21421 gcgatctcac gccgcagagg atccatcaga tcgggattgc ggaaatgcag cgcattcagg
    21481 ccgatatgct cacgatcgcg cgcaaggaag gcttcgcaga cctcgcagcc ttccgccatt
    21541 cggtcgagac caatgcaaaa tacaaggcca cgtccgcgga gcagatagtc gatgactttc
    21601 gcaagtatgt ggcgcagatg cagcccaaac tgcccgacct gttcattttc atccccggat
    21661 cgccggtgac ggtcgaagcc atgcctgact tccaggcggc gaatgccacg cactatcaaa
    21721 ccgggacgcc ggatggcaaa cgcccgggcc ggatcgttgt cgccgtctcg cacgccacgg
    21781 agcgcatgct ggtcgacgac gaagcggtcg cgtatcacga gggcatcccc ggccaccaca
    21841 tgcaactgtc ggtggcgcag cagctcaccg gtctgcctaa attccgccaa cacagccaga
    21901 actcgggcta catcgagggg tgggcgctgt acgccgaaca gctcggtaaa gaaatcggct
    21961 tctatcagga cccggtgagc gactacggcc gactgtcgag cgaactgttt cgcgctgtgc
    22021 gcctcgtggt cgacaccgga ctgcattcgc aaggctggac tcgcgaccag gtggtcgcct
    22081 tcatgcgcca gtccggcgcg gtcgacgaac ccaccatcca ggcggagaca gatcgctaca
    22141 tttcctggcc ggcgcaggcg ctggcgtata aactcggcca gctcaagttt cgcgaactgc
    22201 gcgaccgcgc ccagaaacag ttgggaccta aattcgaaat ccgcgccttc cacgatgaga
    22261 tgctgagcgg cggtgttcta ccgctcggcc tgttggattc ccgtaccaat agctggattc
    22321 aagagcagaa gcgcaaatga ccacccgaaa acccgtcact ttgcaccggc tgcgcgagat
    22381 gcatgacgcc ggcgaaagga tcgccacgct cacctgctac gacgcggcgt ttgcacgcct
    22441 attggacgag tgcggcgtcg acgtgctgct ggtcggcgac tcgctcggca atgtgctgca
    22501 agggcgtgcg accacgctcg cggtatccat gcaagagatg gcttatcaca cggcttgcgt
    22561 ggcgcggggc aatcgcaccg ctttcatcgt tgccgacatg ccgtttggca gctaccaaga
    22621 atcggccgaa gccgccgtgc gcaacgccgt tctgctcatg caacaaggcg cacagatggt
    22681 caaactcgag ggtggcggct ggacggtgcc gctcgtgcgc ctgctcgtgg agcgaagcat
    22741 tcccgtctgc gcccacctgg gcttcactcc gcagtccgtc catgcactcg gcggttatcg
    22801 ggtacaaggc cgcgacgaga cggcggcacg cgtcttgtgc cgcgacgccg agcaactcgc
    22861 ggcagcgggt gcgtcgctgt tggtgctgga aatggtgcct gcgacactcg cgcgcgacct
    22921 gaccaaggcg ctgtcgattc cgaccatcgg gatcggcgcc ggcgccggct gcagcggcca
    22981 ggtgctggtg ctgcacgaca tgctgggatt tgccggcgat cacccgcggc gcttcgtgcg
    23041 caatttcatg gagggcagcg ccagcatcga agccgcagta cgccaatatg tagccgacgt
    23101 gaaaggggct cgatttcccg acgatgcgct gcatagtttt tgagccgcag tcatgcaagt
    23161 aatccgctcg gtcgccgatc tacgcgcagc gctcacgccc aagcgcaaca tcgtcttggt
    23221 gccgaccatg ggcaatttgc acgcaggtca cctgtcgttg gtgcgcatcg cgcagcaacg
    23281 cggtgacctg gtcgtcacca gcatcttcgt caatcgcctg cagttcgctc cgcacgagga
    23341 tttcgccact tatccgcgca cgttcgaccg cgattgcgag cttctcaccg gcgccggctg
    23401 cgacgtcgtg ttcgctccgc cggatagcga ggtttacccg gagccgcaag ggtttacgct
    23461 gcaaccgccg gcactcggcg gcattctcga gggcgccgcg cggccgggat tcttctccgg
    23521 cgtttgcacc gtggtgctga aactcttcaa catggttgag cccgacacgg cggtgttcgg
    23581 caagaaagac tatcaacagc tgctggtcat ccaggccatg gtgcggcaac tggcgctgcc
    23641 catcgagatc gtaccggcag aaacggttcg cgatgctcag ggtctcgcgc tgtcctctcg
    23701 caatggctat ttgagcgagc cgctgcagac caaggcgtcg cagctgcaag cggcgctcgc
    23761 cgccttggtc gccgctgtca aatccgggcg cacggattgg caggccctcg agcaccaggc
    23821 ggcacaattt cttggcgcca gaggctggct gacggac
//
LOCUS       DASWZT010000278        10211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_94259, whole genome shotgun sequence.
ACCESSION   DASWZT010000278 DASWZT010000000
VERSION     DASWZT010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10211
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94259"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /locus_tag="VGE92_07500"
     CDS             <1..1343
                     /locus_tag="VGE92_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY1313705.1"
                     /translation="ITLYGTVDIGLQNQSHGAPISDYFVGGSAEVVQKNSNRSVTGLT
                     PSNLSQSRVGIQGNEPIVGDWSGVFKLETFFNPQSGQIADAAKSLVQNNGRPLGTQAD
                     PGSQTTNVDSSASGQTFQQALAGFSSKTFGTVTFGRQNTVLADGIAKYDPNYASQAFS
                     LIGISGTTAGGGDTQNRRLDDSVKYVGTYGIPAGGAVHLSAEYKFNQANGGSANTAYE
                     VSFGGEFAGFSGDVYYTKIHDAVSLAALSAAQVADLATLCPNPIPAGGSCIAASPSNA
                     LAATISDNTSYAFMGLYNMGFLKFFGGYEHIQYANPNTPLVGGFNVAAYTVAYVNVQS
                     GPKSTYLNDKDLQVFWAGIRWTVIPDLDLVGAYYGYKQDAYATGADAGCSTKLAGTCS
                     GTENVISLDADYRLTKRFDAYIGSMYSGVKDGLAAGYPYHTTDITTTLGVRFKF"
     gene            1453..2493
                     /gene="pstS"
                     /locus_tag="VGE92_07505"
     CDS             1453..2493
                     /gene="pstS"
                     /locus_tag="VGE92_07505"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF008171.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEY1313706.1"
                     /translation="MRIFRPLIVAAFAASLSFAAAAADISGAGATFPYPIYAKWADAY
                     KKLTGVGLNYQSIGSGGGIKQIKAKTVTFGASDMPLKPDDLKTAGLLQFPMIIGGVVP
                     VVNIKGIQGGQLHLDGATVASIYLGDITQWNDPKIKSLNPKLALPATAIAPVYRSDGS
                     GTNFLFSDYLSKSSPKFLSTIGANTSVQWPTGIGAKGNEGVANMTTQTDGAIGYVEYA
                     YAKQNKMAYTLLTNKSGAAVAPNAESFQAAAANADWVHADAYYLILTDQAGAKSWPIT
                     GASFILVYKAPGDPAAVNEALKFFAWAYQDGANMAAELDYVPLPAPLIAQVKKTWTSE
                     ITSAGHPVWSGK"
     gene            2519..3478
                     /gene="pstC"
                     /locus_tag="VGE92_07510"
     CDS             2519..3478
                     /gene="pstC"
                     /locus_tag="VGE92_07510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEY1313707.1"
                     /translation="MAIIDPRHAAKQLIYERIFRAATLSAAILVLLILGGVAVSLGSG
                     AWPALRHFGLSFVTREIWNPVTDEYGALAPIYGTLVTSSIAMLLAVPAGFGIALFLTE
                     LCPPLLKRPIGVAVELLAAVPSIIFGIWGLFVLAPVLQRYVQPWLISHIGHLPFVGSL
                     FKGPPFGIGILTAGVVLAIMVLPFIAATMRDVFDTVPPMLKESGYGLGATTWEVMWNV
                     VVPHSRVGIVGGVMLGLGRALGETMAVTFVIGNAHRISTSLLAPGTTISSSIANEFTE
                     AVGDLYTSSLIALGLLLFLITFTVIAAAQAMLRRLERRGSVAV"
     gene            3475..4329
                     /gene="pstA"
                     /locus_tag="VGE92_07515"
     CDS             3475..4329
                     /gene="pstA"
                     /locus_tag="VGE92_07515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEY1313708.1"
                     /translation="MKRRAVRKAKNTVFLTLSLVATMTGLACLAAILWTLLSHGLAGM
                     SWHLFAQMTPPPGGRGGLLNALYGSAVMTLLGILIGSPIGVLAGTYLAEYGRNSRLST
                     VIRFINDVLLSAPSIIIGLFVYEIVVVRMGHFSAIAGALALAILAIPVTLRTTEDMLN
                     LVPIGMKDSSAALGAYPWRTTISVLYPAARSGIVTGLLLAIARISGETAPLLFTALNN
                     QFWSTQLNAPMANLPVVIFQFALSPYKDWQDLAWAGALIITITILVLSISARVILAML
                     QAERGSGR"
     gene            4362..5162
                     /gene="pstB"
                     /locus_tag="VGE92_07520"
     CDS             4362..5162
                     /gene="pstB"
                     /locus_tag="VGE92_07520"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009088771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEY1313709.1"
                     /translation="MRAPVVAAAEPVKDVGPPKIEIRGLNFYYDQLQSLHNINMSLAD
                     RSISAFIGPSGCGKSTLLRVLNRMYALYPKQRAAGEVLIGGRDILGPTVDVAELRYRV
                     GMVFQKPTPFPMSVFDNVAFGLRLSGGVRGAELKSRVESALRDAAIWDEVKDKLHADG
                     RSLSGGQQQRLCIARAIAVKPEVLLLDEPTAALDPISTLRVEETLQALREKYCIVVVT
                     HNLQQAARVSNVTGFMFMGELVEIGPSSQIFTSPKDRRTDDYITGRFG"
     gene            5242..5988
                     /gene="pstB"
                     /locus_tag="VGE92_07525"
     CDS             5242..5988
                     /gene="pstB"
                     /locus_tag="VGE92_07525"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEY1313710.1"
                     /translation="MTARNVNVHYGDKHAVKNVNLDIGRNQVLALIGPSGCGKSTFLR
                     CLNRMNDTIAAARVDGVITLDGYDIYDRKQDVAQLRARVGIVFQKPTPFPHSIYANVA
                     YGPRIHGLASSRGDMDELVHLSLVRAGLWEEVKDRLSTSGTSLSGGQQQRLCIARTIA
                     VNPEVILMDEPTSALDPIASARIEDLIDELRANYAIVIVTHNMQQAARVSQRTAYFHM
                     GDLIEVGATEVIFTNPQHRLTEDYITGRFG"
     gene            5999..6403
                     /locus_tag="VGE92_07530"
     CDS             5999..6403
                     /locus_tag="VGE92_07530"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY1313711.1"
                     /translation="MKATLQELALVLADSAFLKPFGFVIQACAPGECTLRVPYARSLE
                     RPGAMLSGITIMGAADVTMWLAIMTQRGTAEQWVTRDMKTAFLSGARADILCTARLLK
                     LGKRTAYGTAECRAVNGDLLSHHVLSYALVSA"
     gene            complement(6408..7238)
                     /gene="dapD"
                     /locus_tag="VGE92_07535"
     CDS             complement(6408..7238)
                     /gene="dapD"
                     /locus_tag="VGE92_07535"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEY1313712.1"
                     /translation="MAHAQLQAAIENAFETRASITPRNVSAEVRAAVDTVLGLLDEGK
                     LRVAQKHGADWRVNEWLKKAVLLSFRIADNAPVQAGALNFYDKVPLKYQGWDTGRFAA
                     AGARIVPPATVRRGAHIASNVVLMPSYVNIGAYVDSGTMVDTWATVGSCAQIGKNVHL
                     SGGVGIGGVLEPLQAAPTIIEDDCFIGARSEIVEGVVVESGAVVSMGVFIGQSTRIYD
                     RERDEILYGRVPSGAVVVPGSLPARNGKYALACAVIVKRVDAQTRAKTSLNELLRNTD
                     "
     gene            complement(7241..9916)
                     /gene="glnD"
                     /locus_tag="VGE92_07540"
     CDS             complement(7241..9916)
                     /gene="glnD"
                     /locus_tag="VGE92_07540"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEY1313713.1"
                     /translation="MNAPEKILAHPADWPYLTAAPLALQQGQFSPIVFRQVLDRGSEL
                     LQERFAHDDAIEDLVRDRARLVDITLRAAWVRHAGRFADDLALVAVGGYGRGELHPSS
                     DIDIMVLLPKSDSADWQSDIEKFLTFLWDIGLEVGHSVRSIDDCQRESLADISVATTL
                     FEARLLAGPEALFAGMRRALAPDRLWSSQDFFEAKVKEQTERHHRYFDTAYNLEPNVK
                     SSPGGLRDIQTIGWVAKRHFGTDTLDELVAHGFLTREELRKLKAGQSFLWKVRFGLHV
                     LTGRREDRLLFDYQIKLAKMFGYEDASFTLAVEQLMQKYYRTVMDISLLNEMLLQLFR
                     EAILTQSGPPRPINPRFQIRNDYLEVTHPEVFARYPSALLEMFVLLQQHPELRGVRAE
                     TIRLVGTHVWLIDEEFRQNPRHHRLFMEILCAPVGVTHEMRRMNLYGVLGRYIPSFGR
                     VVGRMQYDLFHAYTVDAHTLFVVSNLRRLAIPKFNDEFPALSQIMQSLPRQEIAYLAA
                     LFHDIAKGRGGDHSELGAVDAEAFCLEQGLGRYEARLVAWLVRNHLILSITAQKKDIS
                     DPDIIHDFARRVGDQAHLDYLYVLTVADVRGTNPKLWNAWKARLFEEFYERTKRALRR
                     GLETPVDQEELIRETQEQAREKMPNVDPAKIARVWAQWTEAYFLRYTPEEIAWQTAVL
                     ADRDPLDDAPLVAIRQLAQRGGTAVLTYSPRRLRSFARTTAVLDQMGLNVVDARLITS
                     ANGFSLETYVVLEDNGIVIADEDRLREIEQGVWRTLRQREDAPATVTRRAARQVRMFS
                     TPTQVNFSVDSRNGRTILELIAADRPGLLSEVGKVFRTERVAINGAKIMTVGERAEDV
                     FYVTDADGRLLPEEACQRLQESLVRALDRRDTARV"
     gene            complement(9913..>10211)
                     /locus_tag="VGE92_07545"
     CDS             complement(9913..>10211)
                     /locus_tag="VGE92_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005312070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEY1313714.1"
                     /translation="REYCGHGIGRIYHEDPQVLHYGKPGTGLELAAGMTFTIEPMINA
                     GRRDVRLLPDGWTVVTKDQSLSAQWEHTILVTDLGFEVLTLSASVGLPAAAAAS"
BASE COUNT         1904 a         3141 c         3152 g         2014 t
ORIGIN      
        1 gcatcacctt gtacggcacc gtagacatcg gtctgcaaaa tcagagccat ggcgcgccca
       61 tcagcgatta cttcgtcggc ggcagtgcgg aagtcgtgca gaagaacagc aatagatcgg
      121 tgaccggcct tacgcccagc aacttgagcc agtcgcgagt tggcattcaa ggcaatgagc
      181 ccatcgtcgg cgattggtcg ggcgtgttca agctcgaaac ctttttcaat ccgcagtccg
      241 gccagatcgc cgacgccgcg aagtctctgg tacagaataa tggccggccc ttgggtacac
      301 aggccgaccc tgggagccag accaccaatg tggattcgag cgcgtcgggc cagacttttc
      361 agcaggcact ggcggggttc agctcgaaga cctttggcac ggttaccttc ggccgtcaaa
      421 acacggtgct ggccgacggc atcgccaagt acgatcccaa ctatgcctcg caagccttct
      481 cgctgatcgg catatcggga accacggccg gcggcggcga tacccagaat cgccgcttgg
      541 atgattcggt gaagtacgtg ggtacgtatg gcataccggc gggcggcgct gtgcacctgt
      601 ccgcggagta caaattcaat caggccaacg gcggcagcgc caacaccgct tacgaggtgt
      661 ccttcggcgg cgagtttgcg ggcttctccg gcgatgtgta ctacaccaag atccatgacg
      721 cagtctctct cgccgccctc agcgcggcgc aggtcgccga cctcgcgacg ctgtgcccca
      781 acccgatacc tgccggcggc agctgcatcg cggccagccc gagcaacgcc ctggctgcga
      841 ccatttcgga caacacgagc tatgctttca tgggcctgta caacatgggc ttcttgaagt
      901 tcttcggcgg ctacgagcat atccagtacg cgaatccgaa taccccgctg gtcggcggct
      961 tcaacgtggc cgcctacacg gtcgcctacg tcaacgtgca aagcgggccc aagtcgacct
     1021 acctcaacga caaggacctg caggtattct gggcgggcat acgctggacc gtgatcccgg
     1081 atttggacct ggtcggcgcc tattatggtt ataagcagga cgcgtacgcc accggcgcgg
     1141 atgccggctg ttcgacgaag ctggccggaa cctgcagtgg caccgagaat gtgatttcac
     1201 tcgacgccga ctatcgcttg accaagcgtt tcgatgcgta catcggctcg atgtactccg
     1261 gcgtgaagga tggccttgcg gcgggatacc cgtaccatac gaccgatatc acgacgacgc
     1321 tgggcgtgcg cttcaagttc tagcggtccg tacaaaatcg acttcatggc cctatttttt
     1381 ggggccatga ggtcgacaca cgcagctggg ccattggtct ggcccaagag tcgctcaagc
     1441 cggagcaatg ctatgagaat tttcaggcct ctgattgtcg cggcatttgc cgcatccttg
     1501 agcttcgcgg ccgcggcggc cgacatctcg ggcgccggcg ccacatttcc ctatcccatt
     1561 tacgccaaat gggcggatgc atacaagaaa ctgaccggcg ttggcctcaa ttatcaatcc
     1621 atcggatcgg gtggcggcat caaacagatc aaggctaaga ccgtgacctt cggcgcctcg
     1681 gacatgccct tgaaaccgga cgatctgaaa accgccggcc tgctgcagtt tccgatgatc
     1741 atcggcggcg tggtgccggt ggtcaacatc aaaggcattc aaggcggcca attgcacctc
     1801 gacggcgcga cggtggcgag tatttacctg ggcgacatca cgcagtggaa tgacccgaaa
     1861 atcaagagct tgaatccgaa gctggcgttg ccggcgacgg cgatcgcccc ggtttaccgc
     1921 tcggacggtt ccggcacgaa tttcttgttc tccgattacc tgtcgaagtc gagtcccaag
     1981 ttcctgtcca ccatcggcgc taatacctcg gtgcaatggc cgacaggaat cggcgccaaa
     2041 ggcaacgagg gcgtcgccaa catgaccaca cagaccgacg gcgcaatcgg ctacgtcgaa
     2101 tacgcctacg ccaagcaaaa caagatggcg tacaccttgc tcaccaataa atcgggagcc
     2161 gccgtggcac ccaatgcaga gtcctttcag gcggcagccg cgaacgcgga ttgggtgcac
     2221 gcggatgcct attatctgat cctgacagac caggccgggg ccaagagttg gccgatcacg
     2281 ggcgcgagct tcattctggt ctacaaggcg ccgggggatc cggcggcggt caacgaagcc
     2341 ctcaagttct ttgcctgggc gtatcaggat ggcgctaaca tggcggccga attggactac
     2401 gtgccgttgc ccgccccgtt gatcgcgcag gtcaagaaga cctggacctc ggaaatcacg
     2461 tcggcgggtc atccggtgtg gagcggcaaa tagcgaccgc gggagcgaaa ttgacatcgt
     2521 ggcgatcatc gatccgaggc acgccgcaaa gcagttgatt tacgagagaa tcttccgcgc
     2581 cgcgacattg agcgcggcca ttctcgtgtt gttgatcttg ggcggagttg cagtctcctt
     2641 gggcagcggt gcctggcccg cattacggca tttcggcttg tccttcgtca cccgcgagat
     2701 ttggaatccg gtaacggacg agtatggcgc gttggcgccg atctatggaa cgctggtcac
     2761 ttcatcgatt gcgatgctgc tggcggtgcc ggcggggttt ggaatcgccc tgttcctgac
     2821 cgagctgtgc ccgccgttat tgaagcggcc gataggcgtc gccgtcgaat tgctcgcggc
     2881 ggtaccgagc atcattttcg gcatttgggg gttgttcgtg ctcgcgcctg tgctgcagcg
     2941 ctatgttcag ccgtggctga tcagtcacat cgggcacttg cccttcgtcg gtagtctgtt
     3001 caagggcccg ccgttcggca tcggcattct caccgcgggc gtggttctcg caatcatggt
     3061 gttgccgttc atcgcagcca ccatgcgcga tgttttcgac accgttcccc ccatgctgaa
     3121 agaatccggt tacggcctcg gcgcgaccac ctgggaagtc atgtggaacg tggtcgtgcc
     3181 gcactcgcgc gtcggcattg tgggtggtgt gatgttgggt ctggggcgcg cgctcggcga
     3241 gaccatggcc gtgaccttcg tgatcggcaa cgcgcatcgc atcagcacgt ccttgttggc
     3301 tccgggcacg acgatttcct cgtccatcgc caacgaattc acggaggcgg tcggcgatct
     3361 gtatacctcc tcgctgattg cgttgggtct gttgttgttt ctcatcacct tcaccgtgat
     3421 cgcggcggcg caggcgatgc tgcgacgcct ggaacgccga ggatcggttg cggtatgaaa
     3481 cgacgcgcgg tgcgcaaggc caaaaatacc gtctttctga cgttgtccct ggttgcgacc
     3541 atgaccggat tggcctgcct ggctgccatc ctctggacct tgctgtcgca tgggctcgcg
     3601 ggcatgtcat ggcacctgtt cgcgcaaatg acgccgccgc cgggtggccg tggcggcttg
     3661 ctcaacgcgc tttacggcag cgccgtgatg acgctgctcg gtattctgat cggctccccg
     3721 atcggcgtgt tggcaggaac ttatctcgcg gaatacggcc gcaactcgcg cctcagcacg
     3781 gtcattcgct tcatcaatga cgtgctgttg agcgccccct cgatcatcat cggcttgttc
     3841 gtttacgaga tcgtggtggt gcgcatgggt catttttcgg ccatcgccgg cgcgttggct
     3901 ctcgcgatcc tggcgattcc ggtgacattg cgcaccaccg aggatatgct gaatctggtg
     3961 cccatcggca tgaaggactc ttcggccgcg ctcggggcgt atccctggcg caccacgatc
     4021 agcgtgctgt atccggcggc acgcagcggc atcgtgacgg gattgttgct ggctatcgcg
     4081 cgcatcagcg gcgaaactgc gccgctgctg ttcacggcgc tcaacaatca attttggagc
     4141 acgcaactca acgcgcccat ggcgaacctc ccggtggtca ttttccagtt tgctttgagc
     4201 ccctacaagg attggcagga tctggcctgg gccggcgctc tgatcatcac gatcacaatc
     4261 ttggtgctga gcatttccgc gcgagtgata ctggcgatgc tccaggcgga gcgagggtcc
     4321 ggccgatgat gccgtcgacg aaaccaccga agcatctgaa gatgcgcgcg cccgtcgtgg
     4381 cagccgcgga gccggtgaaa gacgtagggc cgccgaagat cgaaatccgc ggcttgaatt
     4441 tctactacga ccagctgcag tcgctgcaca acatcaacat gtcgctggcc gaccgttcga
     4501 tcagtgcatt catcgggccg tcgggctgcg gcaaatcgac gctgcttcgc gtcctgaatc
     4561 gaatgtacgc gctgtaccca aagcagcgcg cggcgggaga ggtgctcatc ggtggacgcg
     4621 acatcctggg ccccacggtc gacgtcgccg aattgcgcta ccgcgtcggc atggtattcc
     4681 agaagccgac accgtttcca atgtcggtgt tcgacaacgt cgcattcggc ctgcgcctga
     4741 gcggcggtgt gcgcggcgcc gaactgaaat cgcgcgtcga gtcggcgctg cgcgacgcgg
     4801 cgatctggga tgaggtcaag gacaagctgc atgccgacgg ccgcagtctg tccggagggc
     4861 agcagcaacg gctgtgcatc gcgcgcgcga ttgcggtcaa gcccgaagtg ctgctgctcg
     4921 atgagccgac cgcggcgctc gatccgattt ccacattgcg tgtcgaggaa acgctgcagg
     4981 cgttgcgtga gaagtattgc atcgtcgtgg tgacgcacaa tctgcagcag gcggcgcggg
     5041 tttcgaatgt gaccggattc atgttcatgg gagaactcgt ggaaataggc ccttccagcc
     5101 agatcttcac ctcgccgaaa gatcgaagaa ccgacgatta cattaccgga cgattcggct
     5161 gagctgatcc agatgaacga ttaccccgtc aatgacggca gggccgccgc aagcgtccgc
     5221 gatgcgctgc gcgacgccgt catgacggcg cgcaatgtca atgtgcacta cggcgacaag
     5281 cacgccgtca agaacgtaaa tctcgatatc ggccgcaacc aggtgcttgc cctgattgga
     5341 ccgtcggggt gcggaaaatc gacgtttctg cgctgcctca atcgcatgaa cgacacgata
     5401 gccgcggcgc gcgtcgacgg cgtgattact ctcgatggct acgacatcta cgatcgtaag
     5461 caagacgtgg cgcagctgcg ggcgcgcgtc ggcatcgtgt ttcaaaagcc cacgccgttt
     5521 ccgcactcga tttatgccaa cgtcgcgtat ggaccgcgaa ttcacggcct ggcgtcgagc
     5581 cgcggcgaca tggatgaatt ggtgcacctt agtttggtgc gcgccggact gtgggaggag
     5641 gtgaaggatc ggctgtcgac gtccggcacc agtctttcgg gcgggcagca gcagcggctg
     5701 tgcatcgcgc gcacgatcgc ggtgaatccg gaggtcatat tgatggacga gcccacgtcg
     5761 gcgctcgatc caatcgcgag cgcgagaatc gaggatttga tcgacgagct gcgcgcgaac
     5821 tatgccatcg tgatcgtgac gcacaatatg caacaggccg cgcgcgtctc gcagcgcacc
     5881 gcgtattttc acatgggcga tctgatcgaa gtgggtgcaa ccgaggtgat tttcaccaat
     5941 ccgcagcaca gactcacgga ggattacatt accgggcgct tcggttaagg aatatctggt
     6001 gaaggctacg ttgcaagaat tggccctggt cctcgcagac tcggcgtttc tcaaaccttt
     6061 cggcttcgtc attcaagcat gcgcgcccgg cgagtgcacg ctgcgggttc cttatgcgcg
     6121 ctcgctggag cggccgggcg ccatgctctc gggcatcacc atcatgggcg cggccgacgt
     6181 gacgatgtgg ctcgcgatca tgacgcagcg cggcaccgcc gagcagtggg tgacccgtga
     6241 tatgaaaacc gcatttctca gcggtgcgcg cgccgacatt ctgtgcacgg cgcgcttgct
     6301 caagctggga aagcgcaccg cctacggtac tgccgagtgt cgtgcggtca acggcgatct
     6361 attgtcgcac catgtcttga gctacgcgct ggtcagcgct tgattcctca gtcggtgttg
     6421 cgcagcagct cgttcaatga agtcttggcg cgagtttgcg catccacccg cttgacgatt
     6481 accgcgcacg cgagtgcgta cttgccgttc ctggcgggca gcgagccggg cacgaccacc
     6541 gcgcccgagg gaacgcggcc gtacaggatt tcgtcgcgct cgcgatcgta aattcgcgtg
     6601 ctctggccga tgaatacgcc catggaaacc accgcgcccg attcgacgac tacgccttcg
     6661 acgatttccg agcgcgcgcc gatgaaacaa tcatcctcga tgatggtcgg cgcggcttgc
     6721 agcggctcga gcacgccgcc gatgccgacg ccgccggaca ggtgcacatt cttgccgatc
     6781 tgcgcacagg agcctacggt tgcccaagtg tcgaccatgg tgcccgagtc gacgtacgcg
     6841 ccgatgttga cgtagctcgg catcaagacc acgttgctcg caatgtgcgc gccgcgccgc
     6901 accgttgccg gcgggacgat gcgcgcgccg gccgcggcga atcggcccgt gtcccaacct
     6961 tgatatttga gcggcacctt gtcgtaaaaa ttcagcgcgc cggcttgcac cggggcattg
     7021 tccgctatgc gaaatgacag cagtaccgcc ttcttcagcc actcattgac ccgccaatcc
     7081 gcgccgtgct tctgcgcgac gcgcagcttg ccctcatcga gcaagccgag cacggtgtca
     7141 acggcggcgc ggacctcggc ggaaacgttg cgcggcgtga tgctggcgcg cgtttcgaag
     7201 gcattctcga tggccgcttg tagttgcgcg tgcgccaatt tcaaactcga gccgtgtcgc
     7261 gccggtcgag cgcgcgcacc agtgattctt gcaagcgctg acacgcctcc tccggcagca
     7321 ggcggccgtc ggcatcggtc acgtagaaca cgtcttcggc acgttcgccc acggtcatga
     7381 tcttggcgcc gttgatcgcg acgcgctcgg tgcgaaacac cttgcccacc tcggagagca
     7441 gtccggggcg gtccgcggcg atcaactcga gaatggtgcg gccattgcgg ctgtcgacgc
     7501 tgaagttgac ctgcgtcggc gtggagaaca tcctcacctg acgcgccgcg cgccgcgtca
     7561 ccgtcgccgg cgcatcttcc cgttgccgca gtgtgcgcca cacaccctgt tcaatttcac
     7621 gcagacgatc ctcgtccgcg atcacgatgc cgttgtcctc gagcaccacg taggtttcca
     7681 ggctgaagcc attggcgctg gtgatcagcc gcgcgtcgac gacgttcagc cccatttgat
     7741 cgagtaccgc cgtggtgcgt gcgaagctgc gcagccgccg cggcgaatac gtcagaaccg
     7801 ccgtgccgcc gcgctgcgcc agctgtcgaa tcgcgaccag cggcgcgtcg tccaatggat
     7861 cgcggtcggc gagcaccgcc gtttgccatg cgatttcctc gggcgtgtag cgcaggaagt
     7921 acgcttcagt ccactgcgcc cagactcgtg cgatttttgc agggtcgaca ttcggcattt
     7981 tttcgcgcgc ttgctcctgc gtctcgcgga tcagttcctc ctgatcgacg ggagtctcga
     8041 gcccacggcg caaggcgcgc ttggtgcgct cgtagaactc ttcgaacagc cgcgccttcc
     8101 atgcattcca tagcttcggg ttggtgccgc gcacgtccgc gacggtcagt acgtacaaat
     8161 agtcgaggtg cgcctgatcc cccacgcgcc gcgcgaaatc atgaatgatg tcaggatcgc
     8221 tgatgtcctt tttctgcgcg gtaatcgaca ggatcaggtg gtttcggacc agccatgcga
     8281 ccaaacgtgc ctcgtagcgg ccgagaccct gttccaggca aaacgcttcg gcgtcgaccg
     8341 cccccaattc cgagtgatcg ccgccgcggc ccttggcgat gtcgtggaac agcgctgcca
     8401 gataagcgat ctcctggcgc ggcagggact gcatgatctg gctcaatgcg ggaaattcgt
     8461 cgttgaattt cggaatcgcc aagcgccgca gattgctgac cacgaacaag gtgtgcgcat
     8521 ccacggtgta agcgtggaat aaatcgtatt gcatgcgccc gaccacgcgc ccgaaagagg
     8581 gtatgtagcg tccgagaacg ccgtacagat tcatgcggcg catttcgtgc gtgacgccga
     8641 ctggtgcgca gagaatttcc atgaacaggc gatgatgacg cggattctgg cggaactcct
     8701 cgtcgatgag ccagacatgc gtgccaacca ggcgaatcgt ctcggcgcgc acgccgcgca
     8761 gctccggatg ctgctgcagc agcacgaaca tctcgagcaa ggcggacgga tagcgggcga
     8821 agacttccgg atgcgtcact tcgaggtagt cgttgcgaat ctggaagcgt ggattgatcg
     8881 gccgcggcgg tccgctttgc gtcaagatgg cctcgcgaaa cagctgcagc agcatctcgt
     8941 tgaggaggct gatgtccatc acggtgcggt agtacttctg catcagctgt tcgacggcga
     9001 gcgtgaaact cgcgtcctcg tagccgaaca tcttcgctag tttgatttga tagtcgaata
     9061 gcagccgatc ctcgcgccgc ccggtcagaa catgcagtcc aaagcgcacc ttccacagga
     9121 acgattgtcc ggctttgagc ttgcgcagct cctcgcgcgt gaggaagccg tgtgccacca
     9181 gctcgtcgag cgtgtcggtg ccgaagtggc gcttcgcaac ccagccgatc gtctgaatgt
     9241 cacgcagccc gcccgggctc gatttgacgt tcggttcaag gttatacgcg gtatcgaagt
     9301 agcgatgatg ccgctcggtc tgttccttga ccttcgcctc gaagaaatcc tgcgaggacc
     9361 acaagcggtc gggcgccaac gcgcgccgca tcccggcgaa tagcgcctcg gggccggcca
     9421 gcagccgcgc ctcgaacaag gtggtggcga cgctgatgtc ggcgagactc tcgcgctggc
     9481 agtcgtcgat ggaacgcaca ctgtgcccga cctcgaggcc gatatcccag agaaaggtca
     9541 gaaacttctc gatatccgat tgccagtccg ccgaatcgct ctttggcagc agcaccatga
     9601 tgtcgatatc cgaactcggg tgcagttcgc cgcgtccgta gcctcctacc gccaccagcg
     9661 caagatcatc cgcaaaccgg ccggcgtggc gcacccaggc ggcgcgcagc gtaatgtcga
     9721 ccaggcgcgc gcggtcgcgc accaagtcct cgatcgcgtc gtcgtgagcg aaacgctcct
     9781 gcaaaagctc actgccgcga tccaagacct gacggaaaac gatcggcgag aattggccct
     9841 gctgtagggc gagaggtgct gccgtcaaat acggccaatc ggcagggtgg gcgagaatct
     9901 tttcgggcgc gttcaagagg ccgccgcggc ggctggaagg cctacgcttg cgcttaaagt
     9961 gagcacttcg aagcctaagt cagtcaccaa gatcgtgtgc tcccactgcg ccgacagcga
    10021 ttgatccttg gtcaccacgg tccagccgtc ggggagcagc cgtacgtcgc ggcgcccggc
    10081 attgatcatg ggttcgatcg tgaaggtcat gcccgccgcc agttcgaggc ccgtcccggg
    10141 cttgccgtag tgcagcactt gcggatcttc gtgatagatg cggccgatac cgtgcccgca
    10201 atattcacgc a
//
LOCUS       DASWZT010000279         1645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_69915, whole genome shotgun sequence.
ACCESSION   DASWZT010000279 DASWZT010000000
VERSION     DASWZT010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69915"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..1343)
                     /locus_tag="VGE92_07550"
     CDS             complement(126..1343)
                     /locus_tag="VGE92_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEY1313715.1"
                     /translation="MAVDLFDNWFDPIETEVRARARQFIEELIRGELDTALARPRYER
                     SKIASNEAGACVTGHRHGSRTRSLTGTFGPIEIAVPRARLSTPEGGTAEWKSQALRAY
                     QRRTLAADALIASSYLAGTNTRRVRRALAALFGGTVGKDTVSRTWRKVKGDWDAWNSR
                     SLAEEPIVRLILDGTVVRVRLDRKATSISLLVVMGVRADGQKVLLAIKSMGGESAEAW
                     RTALDDLIKRGLRRPEFLIVDGAPGLDKAIAAVWDGVPVQRCTVHKHRNLLAHSPERL
                     HDEITADYNDMIYATTPEEIAARRKAFLRKWRLKHRAVADSLEEAGERLFTFARLPPS
                     QWRSLRTTNAIERLHEEFKRRIKTQTVLPSADTAAMMFWALLASGQISMRKVDGWQTL
                     ATKPIDQPIDLAA"
     gene            complement(1515..1645)
                     /locus_tag="VGE92_07555"
                     /pseudo
     CDS             complement(1515..1645)
                     /locus_tag="VGE92_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489531.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          273 a          532 c          512 g          328 t
ORIGIN      
        1 atccaaatgt gacccccgtc gcgtacttgg ccgcgcgcta atgttttacc gctcccagcg
       61 cggtgccgtc cggagcgcgg ttggaattcg gtatggcgta tcccgggtga catgaagtta
      121 tcgttttagg cggcaaggtc aattggctga tcaatgggct ttgtggcgag ggtctgccaa
      181 ccgtccacct tgcgcatgct gatctgtccc gaggcgagca gcgcccagaa catcatcgca
      241 gcggtgtcgg ccgacggcag cacggtttgg gttttgatcc gccgcttgaa ctcctcgtgc
      301 aaccgctcga tcgcattggt cgtgcgtaga ctgcgccatt ggcttggcgg caggcgcgcg
      361 aaggtgaaca aacgttcgcc ggcttcctcc aggctgtcgg caacggcacg atgcttgagc
      421 ctccattttc ggagaaatgc cttgcggcga gccgcgatct cctcaggggt cgtggcatag
      481 atcatgtcgt tataatccgc ggtgatctcg tcgtgcaggc gctccgggga atgggcgagc
      541 aggtttctgt gcttgtgaac cgtgcagcgt tgcaccggca cgccgtccca gacggcggca
      601 atggccttgt cgagccctgg tgcgccgtcg acgatgagga attcgggacg ccgcagcccc
      661 cgcttgatga gatcgtccag cgccgtgcgc catgcctcgg cgctttcgcc gcccatgctc
      721 ttgatcgcga gcagaacctt ctggccgtcc gctcgcacgc ccatcacaac gagcagtgaa
      781 atcgaggtcg ccttgcggtc gaggcgcacc cggacaacgg tgccgtcgag gatcaggcgc
      841 acgatcggct cctcggccag cgagcggctg ttccaggcgt cccaatcgcc cttcaccttg
      901 cgccaggtcc ggctcaccgt gtccttgccg accgtcccgc cgaacaaggc cgccagcgcg
      961 cgacgcaccc gcctggtatt ggtgccggcc aggtagctgc ttgcaatcag cgcgtcggcg
     1021 gcaagcgtgc gccgctgata ggcccgcaaa gcctggctct tccattccgc cgtcccgccc
     1081 tccggcgtgc tcagccgggc tcgcggcact gcgatttcga tcggcccgaa cgtgcccgtc
     1141 agcgagcgcg tccggctgcc atgacggtgg cctgtgacgc aggctcctgc ttcattgctg
     1201 gcaatcttgc ttcgttcata acgcggccgt gccagcgcgg tatcgagctc accgcggatc
     1261 agctcttcga tgaactgacg cgcccgagcc cgcacctcgg tctcgatcgg gtcgaaccag
     1321 ttgtcgaaaa gatcaacagc catctctgtc gctggctgca aagaatcaga cttggtggta
     1381 tccttggtca tggcgtggtc tccagtccga cgcttcaacg ccggatgatt cgaggttcat
     1441 caccccggag actacgccaa ccctaattcc taccaactcc gcgacggcac ccgcgcgccg
     1501 atagcggtgc agacagcttc gacggtgcgg gcggcagcct tgcgcagcag atgcttgagc
     1561 tttgcgaaga cctgctcgat cgggttcagg tctggagagt actttggcag aaagaacagc
     1621 ttggcgccgg ttgagcgaat gagcc
//
LOCUS       DASWZT010000280         5110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_31386, whole genome shotgun sequence.
ACCESSION   DASWZT010000280 DASWZT010000000
VERSION     DASWZT010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31386"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /gene="bchF"
                     /locus_tag="VGE92_07560"
     CDS             complement(<1..313)
                     /gene="bchF"
                     /locus_tag="VGE92_07560"
                     /inference="COORDINATES: protein motif:HMM:NF018935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-vinyl bacteriochlorophyllide hydratase"
                     /protein_id="HEY1313716.1"
                     /translation="MRRDASPWTLIQGVLAPIQFVVFLVSAVLVLNFLVTGAGFAAAT
                     TSVVIKTLVLYTIMITGCIWERAVFGRYLFAPAFFWEDFVSMFVLALHTAYLGALLFG
                     SL"
     gene            546..1427
                     /locus_tag="VGE92_07565"
     CDS             546..1427
                     /locus_tag="VGE92_07565"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEY1313717.1"
                     /translation="MCIGDFAEQAGDGSIWEGGGVRGAKDEATPESELRMARLVRTIE
                     GEIVPRLVLARRVARAPDAARVLELKAPDAADIKELVRLLLAHDVGVASAFVQQVRHR
                     GASLEIVCLRLLAPAARELGLLWEEDECDFMQVTVGLCRLHQLLRELSPEFRADETEP
                     KVDRRILLAPIPGEQHTFGITLVAQFLRRAGWDVWHEFPHADTDILEVLRQNWFAVVG
                     LSVGNDTRLENLAAIIRNIRRVSRNRSLGVLVGGPPLVRKPEIAAQVGADATAADGQE
                     AVARAEQICLNQAVNYG"
     gene            1464..2903
                     /gene="ppsR"
                     /locus_tag="VGE92_07570"
     CDS             1464..2903
                     /gene="ppsR"
                     /locus_tag="VGE92_07570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010109 - regulation of photosynthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PpsR"
                     /protein_id="HEY1313718.1"
                     /translation="MKQFSGAKKWLSDLDGETAGRLITVASDVALVVSDVHKGVIRDV
                     AFGSAELAVELADKWVGKPWADTVTADSRPKIEALLRESGAKGAPRWRIVNHHSSSGP
                     DLPVMYAAVEMPKHGHVVAFGRSMQPMATLQQQLVNTQQSMEREYLKLRQAEARHRLL
                     FQVASEAVLIVDAASRKIIEANPAAARLLGEGNKRLVGRLFPDGFDESSTQSLHSLLG
                     AVAATGRTSDVPVRSADGARDFVISASLFREERASFFLVRLTSPTLSHAPGAAATPQS
                     KVLEIVAGAPEGFVVTGLDGRILFANRAFLDLVQLATEEQARNESLERWLGRSGVDFN
                     LLTAQLREHGALRLFATQVRGEYGSNSDVEICAVSVVDGEEPCFGFTIRDVGQRVTFD
                     RQVGRDKPRSVAQLTELVGRVPLKDLVRESTDMIEKLCIEAALELTGDNRASAAEILG
                     LSRQSLYAKLRRYGLGDLASTESEQFDYQ"
     gene            2916..3824
                     /gene="chlG"
                     /locus_tag="VGE92_07575"
     CDS             2916..3824
                     /gene="chlG"
                     /locus_tag="VGE92_07575"
                     /EC_number="2.5.1.62"
                     /inference="COORDINATES: protein motif:HMM:NF005742.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorophyll synthase ChlG"
                     /protein_id="HEY1313719.1"
                     /translation="MSRPQNLALTSDVPAPSVVLELLKPITWFPPMWAFGCGVISSGI
                     APRGHWPMILGGVVLCGPLLCATSQAVNDWFDRHVDAINEPDRPIPSGRMPGRWGLYV
                     AILWTFLSLACAAALGPWVSGVAAVGLALAWAYSAPPMRLKQNGWWGNLACAVCYEGF
                     AWVTGAAVMSGGVTPGGRTLLIAALYSLGAHGIMTLNDFKSIAGDRRSGIASLPVRLG
                     AERAGEVACVAMALPQVAVIALLLAWGRPYHAGCVGALLALQLLFMRRLLADPRARAP
                     WYNGTGVSLYVLGMLVAALALKGLAA"
     gene            3821..>5110
                     /locus_tag="VGE92_07580"
     CDS             3821..>5110
                     /locus_tag="VGE92_07580"
                     /inference="COORDINATES: protein motif:HMM:NF015185.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCD family MFS transporter"
                     /protein_id="HEY1313720.1"
                     /translation="MSDARAPLRWPGILRLGLVQASLGSIVVLVTSTLNRVMVVEYTL
                     PAILPGMLVALHYAVQLIRPRFGHGSDVGGRRTPWIVAGMALLALGAVLCGFATINLA
                     RSFVPALALAVTSYALVGFGVGAAGTSLLALMASRVQENRRAAAAACMWILMIAGFAV
                     TSTAVGHFLDPFTPRRLLSVTGTAAGIALLTALCAVWNVEGAATIAVSAARAARPLSF
                     KSALRQVWSEPRARGFTLFVFVSMLAYSAQELLLEPFAGLLFGYSVGKSAQLSGLWHA
                     AVLAGMVGLGVACSGTRRFVSLRAWTIGGCSVSAVALVCLAAAAVVGPAWPLRATVAA
                     LGAGNGVFAVAAIGSMMELANRDSASAGVRLGLWGAAQAAAFALGGFIGTFALDAVRY
                     FFGSPMIAFAVVFACEALLFFAAARIAARAGSNAAPLA"
BASE COUNT          829 a         1477 c         1778 g         1026 t
ORIGIN      
        1 gcaacgagcc gaataacaac gcgcccagat acgcggtgtg taacgcgagc acgaacatgc
       61 tgacgaagtc ttcccagaaa aatgccggtg cgaacaagta acggccgaag actgcgcgct
      121 cccaaatgca gccggtgatc atgatcgtgt aaagaacgag ggtctttatg acaaccgatg
      181 tcgttgccgc agcgaacccc gcaccggtca ccagaaaatt cagcaccaga acggcgctga
      241 ccaagaacac cacgaattgt atgggagcaa gtactccttg gatcaaagtc cagggtgagg
      301 catcgcgcct caatcgttgc tccggcgtgt acagcggcat cgtattcgaa ccctagtccc
      361 gttgcctccc gtctgtcaac aaaactgtac gtaaagcaaa cttgacaact cgggacgaag
      421 ttgagatggt cacgggacgc cggtcggagt cgattttcgc gctaacgagg gagcagcctt
      481 aaggacgagc aaatagggca accggcgggg gttttacccg cgttctcatt ggagggttgg
      541 gaccaatgtg catcggcgac tttgcggagc aggcggggga cgggagcatt tgggagggtg
      601 gcggcgttcg cggcgccaag gatgaggcga caccggagtc ggaattgcgg atggcgcggc
      661 tggtgcgcac catcgaaggc gagatcgtgc cgcggttggt gctggcgcgg cgggtggcga
      721 gggcgccgga tgcggccaga gtgcttgaac tcaaggcacc ggacgcggcc gacatcaagg
      781 aattggtgag actgctgttg gcgcacgacg tgggagttgc ctcggcgttc gtgcagcaag
      841 tgcgccatcg gggcgcatcc ttggaaatcg tttgcctgcg gctgctcgcg cccgcggcgc
      901 gcgagttggg cctgctctgg gaagaggacg aatgcgactt catgcaggtg acggtgggtt
      961 tgtgccgtct gcaccagctg ttacgggagc tgagccccga gtttcgcgcc gacgagacag
     1021 aaccgaaggt tgatcgacgg atcttgttgg ctccgatccc cggggagcaa cacaccttcg
     1081 gtatcacctt ggtggcccag ttcctgcggc gtgcggggtg ggacgtgtgg cacgagtttc
     1141 cacacgcgga cacggacatt cttgaggttt tacgtcagaa ttggtttgca gtcgtcggat
     1201 tgtccgtggg aaacgatact cggctggaga accttgcggc gatcattcgc aacatacggc
     1261 gggtctcgag gaaccgctcg ctgggagtct tggtcggcgg cccgccgttg gtgcgcaagc
     1321 ccgagattgc agcgcaggta ggagccgacg cgacggcggc ggatgggcag gaggcggtcg
     1381 ccagggccga acagatctgc ctcaaccagg cggtgaacta cggatgattg cggtttcaac
     1441 tttaagtgcg gcaatgaggt cttgtgaagc agtttagcgg tgcaaagaag tggttgagcg
     1501 atctcgacgg cgagacagcc ggccggttga tcaccgtggc gtcggatgtg gcgctggtgg
     1561 tttccgatgt ccataaaggc gtgattcgcg acgtagcttt cggcagcgcg gagctggcgg
     1621 tcgagttggc cgataaatgg gtgggtaagc cgtgggctga taccgtcacc gcggacagcc
     1681 gcccgaagat cgaagccttg ttgcgcgagt cgggcgccaa gggcgcgccg cgttggcgca
     1741 tcgtcaatca ccattcgagc agcggccccg atctgcccgt gatgtacgcc gcggtcgaaa
     1801 tgccgaagca tgggcatgtg gtggcgttcg gccgcagcat gcagccgatg gcgactttgc
     1861 agcagcagct cgtcaatacc cagcagtcca tggagcgcga atatttgaag ttgcgccagg
     1921 cggaagcacg tcaccgtttg ctatttcaag tcgcatcgga agcggtgctg atcgtcgatg
     1981 ccgcatcgcg caagatcatc gaggccaatc cggctgcggc tcgattgctc ggcgagggca
     2041 ataagcgcct cgtgggacgc ctgttcccgg atggattcga cgagtccagc actcaatccc
     2101 tgcacagcct gctgggtgcc gtggccgcga ccgggcgcac cagcgacgtg ccggtgcgat
     2161 ccgccgacgg cgcacgagac ttcgtgattt cagcatcgtt gttccgcgag gagcgcgcgt
     2221 catttttcct ggttcgtttg acgtcgccga ccttgagcca cgcgcccgga gcggcggcga
     2281 cgccgcaatc gaaggtgctg gaaatcgtcg ccggcgcgcc ggaaggtttc gtggtcaccg
     2341 gtttggatgg acggattctg tttgccaatc gcgctttcct tgatctggtg caattggcga
     2401 ccgaagagca agcgcgcaat gagtccttgg agcgctggct gggccgttcc ggcgttgact
     2461 tcaacctgtt gaccgcgcaa ttgcgcgaac acggcgcact gcgcctgttt gcaacccagg
     2521 tgcgcggcga gtacggctcg aacagcgacg tcgagatctg cgcggtgtcg gtggtcgacg
     2581 gcgaagagcc ctgcttcggt ttcaccatcc gcgatgtcgg ccagcgggtc actttcgatc
     2641 gccaggtggg ccgcgacaaa ccgcgctccg tggcgcaatt gaccgagttg gtgggccgcg
     2701 taccgctcaa agatctggtg cgggagtcca ccgacatgat cgaaaagcta tgtatcgagg
     2761 ccgccttgga gttgaccggc gacaatcgcg cctccgcagc ggagatcctg ggcttaagcc
     2821 gccaaagctt gtacgcgaaa ctgcgccgct acggcctcgg agatctcgct tcgaccgaaa
     2881 gcgaacagtt cgattatcaa tagcgaggcg agtcgatgtc ccgtccgcag aacctcgctc
     2941 tgacctcgga tgtgccggcg ccctcggtgg tgctggaact gctcaagccg atcacttggt
     3001 ttccgccgat gtgggctttc ggatgcggcg tgatttcttc gggaattgcc ccgcgggggc
     3061 actggcccat gattctcggc ggcgtcgtgc tctgtgggcc gctgctatgc gcgacgagtc
     3121 aggcggtcaa tgactggttc gaccgtcacg tcgacgccat caatgagccc gaccgtccca
     3181 tcccgtccgg ccgtatgccc gggcgttggg gcttgtacgt ggcgattctg tggacgttcc
     3241 tgtcgctcgc atgcgcggcc gcgctcggac cttgggtgtc cggcgtcgcg gccgtcgggc
     3301 tggcgcttgc atgggcgtac agcgcgccgc cgatgagact caaacagaat ggctggtggg
     3361 gaaatcttgc gtgcgcggtc tgctatgagg gcttcgcctg ggtgaccggt gccgcggtga
     3421 tgtcgggcgg cgtcacgccg ggtggcagga ccttgctcat cgccgctctc tacagtctgg
     3481 gcgcgcacgg catcatgact ctcaatgatt tcaaatccat cgcaggcgac cgacgatcgg
     3541 ggatcgcctc gctgcccgtg cggctcgggg cggaacgggc cggcgaggtc gcctgtgttg
     3601 cgatggcctt gccgcaggtc gcggtgatcg cgttgctgct cgcctggggg cggccgtatc
     3661 acgcgggctg cgtcggcgcg ctgctggcgc tgcaattgct gttcatgcgt aggctgctcg
     3721 cggatccgcg tgctcgcgcg ccttggtaca acggtaccgg cgtcagtttg tacgtcctcg
     3781 gcatgttggt ggcggcattg gcgctgaagg gactcgcagc gtgagcgatg cgcgtgcgcc
     3841 gctgcgctgg cccggcattt tgcgtttggg gctggtccag gcgtcgctcg gatccatcgt
     3901 ggtactggtt acctcaacgc tcaaccgcgt catggtggtc gaatacacgc tgccggcgat
     3961 cttgcccggg atgttggtgg cattgcacta tgccgtgcaa ctgatacggc cgcgcttcgg
     4021 gcacggatcg gatgtcggag ggcgccgcac cccttggatc gtcgcgggaa tggcgctgct
     4081 cgcactcggc gcggtactct gcgggttcgc gaccatcaat cttgcgcgaa gttttgtgcc
     4141 ggcgctcgcg ctcgcagtca cctcttatgc gctggtcgga tttggagtcg gtgctgcagg
     4201 cacctccctc ctggccctca tggcgagccg ggtgcaggag aacagacgcg ccgccgccgc
     4261 cgcctgcatg tggatactga tgattgccgg gttcgccgtt acatcgacgg cggtcggaca
     4321 ttttttggac cccttcacgc cgcgacgttt gctgagtgtg accggcacgg cggcggggat
     4381 cgcgttgctg accgctctgt gcgcagtttg gaacgtcgag ggtgctgcga ccatagccgt
     4441 cagcgcggcg cgagcggcgc gaccgctgtc gttcaaatcc gcactgcggc aagtgtggtc
     4501 cgaaccgcgg gcgcgcggct tcacgctatt cgtgttcgtg tcgatgttgg catacagcgc
     4561 gcaggagctt ctgctcgagc cgttcgccgg attgctcttc ggatacagcg tgggcaagtc
     4621 cgcgcagctg tccgggttgt ggcatgcggc ggtgctcgcc ggcatggtcg gcctcggcgt
     4681 cgcctgcagc ggcacccgca ggtttgtttc attgcgagcc tggacgatcg gcggctgtag
     4741 cgtatcggcg gtggccctgg tgtgtctcgc cgccgccgct gtggtcggac cggcgtggcc
     4801 gttgcgagca acggtcgccg cgctcggagc gggcaatggc gtgtttgccg ttgcggcaat
     4861 cggatcgatg atggaattgg ccaaccgtga ttcggccagc gccggggttc ggctgggttt
     4921 gtggggcgcc gcgcaggcgg cagccttcgc gctgggtgga ttcatcggca cattcgcgct
     4981 ggatgcggtg cgttatttct tcggatcgcc gatgattgcc tttgcggttg tgttcgcttg
     5041 cgaagcgctg ctgtttttcg ctgccgcacg cattgcggcg cgcgccggct cgaacgccgc
     5101 gccgctggcg
//
LOCUS       DASWZT010000281         4437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_50486, whole genome shotgun sequence.
ACCESSION   DASWZT010000281 DASWZT010000000
VERSION     DASWZT010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50486"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="VGE92_07585"
     CDS             complement(<1..1178)
                     /locus_tag="VGE92_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313721.1"
                     /translation="MDPPAQFDIEAALERARALLATADDEAAKRVYLDILRADPAHFL
                     ALNELATLAYSGGFRSAARTAYRQAVGHHPGNKIARVNLANLLLDEHDLSGAKLHYEA
                     AIAIDPQFHEAHQGLASVLDELGLDGAEAHRRQGFSGHAVVSKPYRGIGVGVPLLLLV
                     SARGGNIPTRLWIDDRQFAVTAIYAEFHASGCALPPHAVLMNAIGDADLCDVALSRAA
                     EIVARSTAPVINPPASVQRTGRIEMARRAAAIEGVIAPQIRAVSPAALLAANDLEFPL
                     LLRRPGFHTGRHFLMVENRDALRPAIASLGAQALFLIPYLDARGADGMARKYRVMFVD
                     GVAYPLHLAISAHWKVHYFSADMAANAAFRDEERRFLEDMPGVLGQRAMAALAALSSA
                     L"
     gene            1379..2788
                     /locus_tag="VGE92_07590"
     CDS             1379..2788
                     /locus_tag="VGE92_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY1313722.1"
                     /translation="MSRISDAAERRSDEEESSAPRKAKILVVDQDPELRRLTVARLES
                     ADYVVEGVGEAQAALDGCVVARPNLVVMDLRLQGWDGLAFLKELKSRWPQLPVIILTA
                     HGTIPEAVQATQIGAFGFLVKPVSKDELLGQVQRATGGATLTLSEADLRATIVSRSQL
                     MSDRLSIANQAAGCDAPVLLTGEDGTGKELLARAIHAAGARRDKPFVVVSCRDPAPRL
                     EAELFGDNCDGALNRARGGTLLLEEIDDLPIDLQAPLAKALATDDVLPGRILGAVRAE
                     WRLICTTSRDLKSLTDSGAFLKDLYYQINILPIEIPPLGRRREDIPLLVSHFLEQATE
                     PGGQKKIYSPKAIELLATTDWPGNVRQLFDLVKRNVALSHGAVMSKEFVQESLDGRSP
                     SIPTYDEAREKFARDYLSENLQRTAGNVTQAARLAKRSRTDFYKLLARYRLHPDDFKN
                     LESGNPLDPEPDRGKDSED"
     gene            2875..3654
                     /locus_tag="VGE92_07595"
     CDS             2875..3654
                     /locus_tag="VGE92_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1313723.1"
                     /translation="MTLGREVILETRCLVKAFKGFVAVDGVSLQVRRGEIHALIGPNG
                     AGKTTYFNLLTKFLQPTAGEIVYNGANITREKPADVARRGIARSFQICAVFPQLTVLE
                     NVRVALQRFLGTSFYFWRSQSSLRSLDSRAVELLAEVRLETWAQTLATDLSYGRKRAL
                     ELATTLALEPQLMLLDEPTQGMGHEDVSHVTQLIKQVSANRTVLMVEHNMSVVSSIAD
                     TISVLQRGRVIASGAYEDVAKNPQVVEAYMGTSAGALGGPG"
     gene            3654..4361
                     /locus_tag="VGE92_07600"
     CDS             3654..4361
                     /locus_tag="VGE92_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1313724.1"
                     /translation="MADEALKVANLEAWYGESHILHGVELSVREGEVVTLLGRNGSGR
                     TTTLRAIMGLVEARSGSVRIDGVETVRLAPHRIARIGVGYCPEERGIFAALSTEENLL
                     LPPRLKGTEALQLEEIYGIFPQLLERAKSPGTRLSGGEQQMLAVARILRTGARLLLLD
                     EISEGLAPVIVQKLGDVIRTLRGKGFTVVLVEQNFRFAAPLADRFYVMEHGRIVKEFA
                     QAELRANMGLLQEFLGV"
BASE COUNT          846 a         1324 c         1449 g          818 t
ORIGIN      
        1 agcgcactcg agagggctgc gagcgccgcc atcgcgcgct gcccaagcac gcccggcatg
       61 tcctcgagaa aacgccgctc ttcgtcgcgg aatgcggcgt tggcggccat gtcggcgctg
      121 aagtaatgga ctttccaatg ggcggagatg gccaaatgca aggggtaggc aacgccgtcg
      181 acgaacatca cgcggtactt gcgggccatg ccgtcggcgc cgcgggcatc gagatatgga
      241 attaggaaca gtgcctgcgc acccaacgat gcgatcgccg gccgcagggc gtcgcggttc
      301 tcgaccatga gaaaatgccg cccggtatga aagcccggcc gccgcagcaa caggggaaac
      361 tcaagatcgt tcgcggcgag cagtgccgcc ggcgatacgg cgcgaatttg cggcgcgatc
      421 accccttcga tcgccgccgc ccggcgagcc atttcgatgc gtcccgtgcg ctgtactgaa
      481 gcggggggat tgatcaccgg cgcggtgctg cgcgcaacga tttccgcggc acggctcagg
      541 gcaacatcgc aaagatccgc atcgccgatc gcgttcatga gcaccgcgtg cggtggcaat
      601 gcacaacccg aggcgtggaa ttcggcatag atcgcggtca cggcaaattg ccgatcgtct
      661 atccatagcc gggtgggaat attgccaccg cgggccgaca ccagcagcag caacggcacg
      721 ccgacaccga tgccgcggta gggtttgctg accaccgcat gtccggagaa tccctgtcgt
      781 cgatgggctt ccgcgccgtc caggccgagc tcgtcgagta ccgaggcgag cccctggtgg
      841 gcttcgtgga attgcgggtc gatcgcgatc gcagcctcat agtggagctt cgctcccgag
      901 aggtcatgct catcgagcaa cagattcgca agattgaccc gtgcaatttt attgccggga
      961 tggtgcccga ccgcttgacg gtacgcggtg cgcgcggcgg aacgaaatcc accggaatag
     1021 gccagcgtcg ccaactcgtt gagggccaag aaatgcgcgg ggtcggcacg caggatatcg
     1081 aggtagacgc gtttcgcggc ctcgtcgtcc gccgtggcaa gcagcgcgcg cgcgcgttcg
     1141 agcgccgcct cgatatcgaa ttgagctggg gggtccatgt gtcagtacta catcgattgg
     1201 gtgctgttgc taatcgtgtg acgcatgtcg ccctttggcg acgcaatttt ttttacgatc
     1261 tccgggtacg tggaaacccc agtgtaaact agcggtctat gacgttgcgc aaccccaagg
     1321 aggcaaaact ctgaagaagc ccatctcatc gccgccgagg gcatcgctca gcttcggatt
     1381 gagccgaatt tccgacgctg cggagcggcg cagcgacgag gaagagtcat cggcgccgcg
     1441 caaggcaaaa atcctggtgg tcgatcagga ccccgaattg cggcgcctca ccgtcgctcg
     1501 tctcgaatcc gcggactatg tcgtggaagg ggtgggcgag gcgcaagcgg cactcgatgg
     1561 ctgcgtcgtg gcgcggccca atctggtcgt gatggacttg cgattgcagg gctgggacgg
     1621 cctcgcgttt ctcaaggagc tcaagagccg atggccgcaa ctcccggtga tcattctgac
     1681 ggctcacggc acgatccccg aggcggtcca agccacccaa ataggcgcat tcgggttcct
     1741 ggtcaaaccg gtctccaagg atgaattgtt ggggcaggtg caacgcgcga cggggggggc
     1801 gaccctgact ctttccgagg ccgatttgcg cgcgaccatt gtgtcgcgca gtcaactcat
     1861 gtcggaccgg ctgtcgattg ccaatcaggc ggcgggctgt gatgcgccgg ttttgttgac
     1921 cggtgaagac ggcaccggca aggagcttct ggcgcgcgcc atccacgccg ccggtgcacg
     1981 tcgtgataag cccttcgtcg tggtctcctg ccgcgatccc gcgccgcggc tggaggccga
     2041 gctgttcggc gacaactgcg acggcgctct gaaccgtgcg cgcggcggca cgttgctgct
     2101 cgaggaaatt gacgatctgc cgatcgattt gcaagcaccg cttgccaagg cgctggcgac
     2161 ggacgacgtt ctgccgggcc gaatattggg tgcggtacgc gccgagtggc ggctcatctg
     2221 caccacgtcg cgcgacctca aatcgctcac cgacagcggc gcctttctca aagatttata
     2281 ctatcaaatc aatattctgc cgatcgagat accgcccttg ggccgccgcc gcgaggacat
     2341 ccccttgctg gtgtcgcatt tcctggagca agccacggaa cccggcgggc agaagaaaat
     2401 atattcgccc aaggccatcg aattgctcgc gaccaccgac tggccgggaa acgttcgcca
     2461 gctcttcgat ctggtgaaac gcaacgtcgc tctgtcgcac ggcgcggtca tgagcaagga
     2521 gtttgtgcag gagtccctcg atggacgctc gcccagcatc cccacttacg atgaggcacg
     2581 cgaaaaattt gcgcgcgact atctctccga aaatctgcaa cgtaccgcgg gcaatgtgac
     2641 ccaggccgcc cggctcgcca agcgcagccg gaccgatttc tacaagctgc tggcacgcta
     2701 tcgattgcat cccgacgatt tcaagaattt ggagtcggga aacccgctcg acccggaacc
     2761 ggaccggggc aaggacagcg aggattagcc caacttaacg cgaccctcgc atagttctcg
     2821 acagcggtgc gcaaaacagc ttaccgtcgc ctagtccata gagggggcgg gcggatgacc
     2881 ttggggcggg aggtgatcct cgagacacga tgtctggtca aggcgttcaa gggattcgtc
     2941 gccgtcgacg gcgtgtcgct gcaggtacgg cgcggcgaga ttcacgcgtt gatcggcccg
     3001 aacggcgccg gcaagacgac ctacttcaat ttgctcacca agtttctgca gccgaccgcc
     3061 ggcgaaatcg tatacaacgg cgcgaacatt acgcgcgaga agcccgccga tgtcgcccgg
     3121 cgcggcattg cgcgttcctt ccaaatctgc gcggtcttcc cgcagctcac ggtcctcgag
     3181 aacgtgcgag tggcgctgca gagatttctc ggcacctcat tttatttctg gcgttcgcaa
     3241 agcagcttac gcagcctcga cagccgcgcc gtcgagctgt tggctgaagt gcggctcgag
     3301 acatgggcgc aaaccctggc gaccgatctg tcctatggcc gcaagcgcgc gctggaactc
     3361 gcgaccacgc ttgcgctcga accgcaactg atgctgctcg atgagccgac gcaggggatg
     3421 ggccatgagg atgtgtcgca cgtgacgcag ttgatcaagc aggtgtcggc gaatcgcacc
     3481 gtgctcatgg tcgaacacaa tatgagcgtc gtgtcatcca tcgccgatac catttcggtg
     3541 ctgcaacggg gtcgagtgat cgcctcgggc gcttatgagg atgttgcgaa gaatccgcag
     3601 gtcgtggaag cctatatggg aacttcggcg ggcgcgctcg gggggcccgg gtgatggccg
     3661 acgaggcgct aaaagtcgcc aatctcgagg cgtggtacgg cgagtcgcat attttgcacg
     3721 gcgttgaact cagcgtgcgg gagggcgaag tggtgacgct gctcggccga aatggctcgg
     3781 gacgtacgac gacgctgcgc gcgatcatgg gcctggtcga ggcgcgcagc ggctcggtgc
     3841 ggatcgacgg cgtcgagacg gtgcgcttgg cgccgcaccg cattgcccgc attggcgtcg
     3901 gctactgccc cgaagagcgt ggtattttcg cagccctctc gaccgaggaa aatctgctgc
     3961 tgccgccgcg cttgaagggt accgaggcgc tgcagctcga ggagatttac ggtattttcc
     4021 cgcagttgct cgagcgcgcc aagagtcccg gtacgcgctt gtcgggcggt gaacagcaga
     4081 tgctggcggt cgcgcgaatt ctgcgcaccg gtgcacgctt gctgttgctc gatgagatct
     4141 cggaagggct ggcgccggtg atcgtccaga aactcgggga tgtgattcgc acgctgcgcg
     4201 gcaagggctt tacggtggtt ctggtggagc agaattttcg tttcgcggca ccgctcgccg
     4261 atcgattcta cgtgatggag catggccgca tcgtcaagga atttgcacag gccgagctgc
     4321 gggcgaatat ggggttgctg caagaattct tgggtgtctg aagcaaacta acggggatcg
     4381 acgcatgaaa aaaatcatga ctggcgcact cggtgcactg ctgggcctgt acgcggc
//
LOCUS       DASWZT010000282        10822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_60296, whole genome shotgun sequence.
ACCESSION   DASWZT010000282 DASWZT010000000
VERSION     DASWZT010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10822
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60296"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /gene="dinB"
                     /locus_tag="VGE92_07605"
     CDS             complement(<1..1158)
                     /gene="dinB"
                     /locus_tag="VGE92_07605"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002882.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY1313725.1"
                     /translation="MRRSDARAILHIDMDAFYASVEEQDRPELKGKPLIVGGTGGRGV
                     VAAASYAVRRFGVRSAMPMREALRRCPEAICVQPRMMRYREVSERVFAIFHEFTPRVE
                     GLSLDEAFLDVTSSLTLMGSAAAIGREIRRRILAQTGLTASVGVAPNKLLAKIASDLH
                     KPDGMCCIATDNVHAILDGLPIEKLFGVGPKSLPAVHAAGIRTFGDLRLAGDAQVWRA
                     FGRHGNAMRERASGVDDRPVEANREEKSISAEETYATDLRGVKALTAQVLHLADRAAA
                     RLRVHELAAGRVSVKIRRADFTTYTRQRALQPPTRDTAVVSAAATLLLEQWLATQPNA
                     AIRLLGVGVSDLQLPPQRELFGEGAAVSRLDSAIDGIRERFGTGMLTRASLL"
     gene            complement(1162..1584)
                     /locus_tag="VGE92_07610"
     CDS             complement(1162..1584)
                     /locus_tag="VGE92_07610"
                     /inference="COORDINATES: protein motif:HMM:NF025705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HEY1313726.1"
                     /translation="MSRLLLALGVALICGFAVAQDRSPPAANAEVYIIAPHNGATVHG
                     PVTVRFGLKGMGVAPAGVKFDNTGHHHLLVDMDLSEVKLDEPLPATDKLLHFGKGQTE
                     TTLNLAPGKHTLELVFADYLHKSFDPPLHSKKITITVE"
     gene            complement(1690..4152)
                     /locus_tag="VGE92_07615"
     CDS             complement(1690..4152)
                     /locus_tag="VGE92_07615"
                     /inference="COORDINATES: protein motif:HMM:NF009586.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY1313727.1"
                     /translation="MNELAVAAGELLAWAALFLGVLLVLAYRRAGLARSSAVLGLLWL
                     SYWLFGRSPEWWKLLVSVPYLLLLLLNLRPLRRNLLTRPFLRSYRRLLPPMSATEREA
                     LAAGTVWWDGELFSGGPDWQKLMSAKAPALNPAERAFLEGPCEDLCAMLDDWDITHVR
                     ADLPPDVWAFIKEQGFFGMIIPRRYGGLEFSAYAHSCVLCKIASRSGVASSTIAVPNS
                     LGPAELLLHYGTEAQKNHFLPRLARGEEVPCFALTGPRAGSDAASIPDTGVICKGEWQ
                     GAQIVGIKLNFSKRYITLAPVATVIGLAFRLFDPQGLMGGRADLGITCALIPRAAPGV
                     IIGRRHFPLNVPFQNGPIQGHDVFVPLDAIIGGFAMAGQGWRMLVEQLSVGRCISLPS
                     SATGGAQAGVYATAAYARIRRQFNMSIGRFEGVEQVLARMAARTYIMDAARSVTAGAI
                     DRGAKPSVPSAILKYHLTEFSRMIANDAMDVHGGKGICLGPKNYLGRGYQIVPVAITV
                     EGANILTRSLIIFGQGAIRCHPFVLREMNAARNPDHDAGVAEFDSAFLGHVGFAISNA
                     VRSLVMALTFARFTKVPRLPATARYFQHVNRYSASFALAADVAMLTLGGYLKKKEALS
                     ARLGDVLSAMYLASMVLKHYEDQGREAADLPLVEWACRSLLYQAQEQLHLFLRNFPSR
                     PLAACMRLLIFPRGLTYFAPSDRLGSAIADLIMNPTAARERLSRDIYKTQTAHNPLGL
                     LQEALVLSTMAESLEKRIRVDGIKSGQVAALDLPGQIAQALQLGILNQAEAAVLRDYE
                     AKISQLLDVDDFAPHELAAGSP"
     gene            complement(4164..5141)
                     /locus_tag="VGE92_07620"
     CDS             complement(4164..5141)
                     /locus_tag="VGE92_07620"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012439107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY1313728.1"
                     /translation="MKKPVTVTITGAAGNIGYALAFRVASGQMLGADQPINLNLLEIP
                     AASAAVQGVVMELNDCAFPTLNRVTATSDAAIAFKDCNFGMLVGARPRGPGMERKDLL
                     LENAKIFSAQGKALNAVASRDVRVLVVGNPANTNSLIAQRNAPSLPPHRFTAMTRLDH
                     NRGLAQLTEKSGSPLAAVKQIIIWGNHSATQYPDLHHATVSGKPALSVVDAQWFKDTY
                     IPTVQQRGAAIIKARGTSSAASAAAAALDHIHDWMLGTPPGDWVSMAVPSDGSYGIPE
                     GVIYSYPVTCQNGEYSIVQGLSINEFSRDKMQATHRELLEEREGVKDLI"
     gene            complement(5201..5683)
                     /locus_tag="VGE92_07625"
     CDS             complement(5201..5683)
                     /locus_tag="VGE92_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313729.1"
                     /translation="MVPPDDLEEYLRGDSALSRQYKREAAPAPPPALDRHVLRGSGAA
                     PIRSPYLAPLALAASVFLSMALVLAIVFGPQPKKHTDEAPHLVRVAAHSGAAANAPLD
                     RKLRLYSSDPPHTRTPSAWLADIAALRRAGRNNEADAESRRFRAAYPGYSAGEPEFWP
                     "
     gene            complement(5689..6306)
                     /locus_tag="VGE92_07630"
     CDS             complement(5689..6306)
                     /locus_tag="VGE92_07630"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY1313730.1"
                     /translation="MLPSMESLDEDAGLMARYRDGDAAAFLALYARHKGPLYRYLLRQ
                     VRSAAAAADLFQEVWSRLVATRNRYEPRAKFATYLFRIAHNCTIDFFRRDLAMRRAAR
                     SDPADAQAPEPEVPEYQRPDAVAEFLEQQSALLAALGALPREQCEAFLLHEETGLTIE
                     EIARVTDVGLETAKSRLRYAIRKLKKSLHPAQSLARPLRTRTVGT"
     gene            6456..6623
                     /locus_tag="VGE92_07635"
     CDS             6456..6623
                     /locus_tag="VGE92_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313731.1"
                     /translation="MTTQISTKLAALAVALMVNGVLVGGVAFLFNSQLQQQVTALSVA
                     HRAAPATLGAI"
     gene            6695..7099
                     /locus_tag="VGE92_07640"
     CDS             6695..7099
                     /locus_tag="VGE92_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY1313732.1"
                     /translation="MDPNWNDNQPIYRQLRDRMVALILEGALKEGDPLPSVRVVAADY
                     RLNPLTVLKGYQQLVDEHLVEKRRGRGMYVSEGAQRLLLAGERERFLNEEWPQILAVI
                     LRLGFSAEELFAQTPALTHPAPPPRGGLPTEE"
     gene            7108..7965
                     /locus_tag="VGE92_07645"
     CDS             7108..7965
                     /locus_tag="VGE92_07645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1313733.1"
                     /translation="MSALIEASDLTKKYGAHVALDHANFSVQAGRIVGLIGPNGAGKT
                     SALRAILGLTSYEGQLKVLGREPFRERTKLMDEACFIADVAVLPAWLKVDDAIDFVAG
                     VHPRFRRERAAALLAKTQITGERRMRELSKGMKTQVHLALTMAIDAKLLVLDEPTLGL
                     DILARRAFYDALVNDYMDETRTILITTHQVEEIENLLTDVLFIDKGRIVLDSSLDDIA
                     TRYAAVAVAADKSDAARAAGPFYERRMLGKVVLYYEHPDLGELAELGEVHTPSISDLF
                     VAKLSGGVQ"
     gene            7962..8834
                     /locus_tag="VGE92_07650"
     CDS             7962..8834
                     /locus_tag="VGE92_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313734.1"
                     /translation="MNTTIWLVRREFWENRAIWIIPAVIGALLVLAALFGKVNINLPG
                     PLPVHAFGQLFMVTVGGTFFVVMSIYATWYLLDCLYADRRDRSVLFWKSLPISDTLTV
                     LSKLGTALLVIPLVYFAAADATTLIMAFIVSVRAGSTIGAGVWHLDLWVQLQALWIYL
                     IVTTAIWYLPIAGYLLIVSAWAKRAVMLWSILPPIALLIAERWFVGTHVTAQLLWERM
                     VGWVPLAFHINPGEGVWVTSVVDNDTIRTPQSLWSLLNAADFFASPQTWLGVAVGAAL
                     IIGAIQLRMRRTEI"
     gene            8840..9796
                     /locus_tag="VGE92_07655"
     CDS             8840..9796
                     /locus_tag="VGE92_07655"
                     /inference="COORDINATES: protein motif:HMM:NF022825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HEY1313735.1"
                     /translation="MRRSYCPAAVLAAFCASAPGALAEEFFATRDQNPLLRGFYLPLP
                     SDARQDADAALSATLLLSNTLNVESNAHESLFVDGESLALDLAYESALAPGWRYRFSV
                     PLVHDSGGVLDSVIDTWHEVFGFPRGNRPFYPKRQIDYFYAGVGKIDLDHPQSSIGDL
                     AADLGWYAVDDERRTVSVWGGLKAPTGSVADLTSDGAWDSALWAHAALRWPKWQFAAE
                     LGVAQPFGDELFAGKAHRSSEFARLAASRALGATWSLRAQLDGQSARVAGSDLRFLGP
                     SLQLTVGAVRRLSGRWRMQMGFAEDVAVNTAPDITFFLGIHD"
     gene            9846..10328
                     /gene="dps"
                     /locus_tag="VGE92_07660"
                     /gene_synonym="pexB"
     CDS             9846..10328
                     /gene="dps"
                     /locus_tag="VGE92_07660"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: protein motif:HMM:NF006975.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HEY1313736.1"
                     /translation="MYKTKIDLSEKTRRNVIAILNDRLADAIDLQSQVKQAHWNVKGP
                     NFIALHELFDKISDLTLEQIDEIAERVTSLGGTAEGTVAVAAKRSKLKNYPLSITAGK
                     DHLFYLSTQLSVFGKAVRTAIEDTDKLGDADTADLFTGISRDLDKYLWFLEAHLQEKA
                     "
     gene            10343..>10822
                     /locus_tag="VGE92_07665"
     CDS             10343..>10822
                     /locus_tag="VGE92_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313737.1"
                     /translation="MSNSSEPGLASALTTLRGLLTAGETLEAWAVQHRLYALTHRRAC
                     IAATSGRFISLNRRLLGGYESADIRWQDLKETRISAGILAADLTLVAQNSADLNIGSE
                     VNRVWTFSGLRKDQAQAMYRICQQHDQVWREKRRIRELEELRAKSGGVQIGGGQAAYA
                     "
BASE COUNT         2052 a         3426 c         3375 g         1969 t
ORIGIN      
        1 caacaggctg gcgcgagtca acatgcccgt tccgaagcgc tcgcggatgc cgtcgatcgc
       61 cgaatccaat cgcgacacgg cggcgccctc gccgaagagt tcgcgctgcg gcggcagctg
      121 caaatcgctg acgccgactc ccaacaaccg tatagcggcg ttcggctgcg tggccagcca
      181 ttgctcgagc agcagcgtcg cggccgccga aaccacggcc gtgtcccgcg tgggcggctg
      241 caacgcgcgt tgccgagtat aagtggtgaa gtcggcgcgg cggattttga cgctcacacg
      301 gccggcggca agctcgtgca cacgtaagcg agcggccgcg cggtccgcca aatgcagcac
      361 ctgcgccgtg agcgctttga cgccgcgaag atcggtggcg taggtctctt cggcgctgat
      421 cgatttttcc tcgcgattcg cttccaccgg ccgatcatcc acgccgctcg cccgctcgcg
      481 catggcattg ccgtgcctgc cgaacgcccg ccacacctgg gcatcgccgg ccaatcgcag
      541 atcgccgaac gtgcgaatgc ccgcagcgtg cacggcgggc aagctcttcg gcccgacgcc
      601 gaacaatttc tcgatgggca gtccatccaa aatcgcgtgc acgttgtcgg tagcgataca
      661 gcacatgcca tcgggcttgt gcaaatccga cgcgattttc gccagcagct tgttcggtgc
      721 aacgccgacc gacgcggtga gaccggtttg cgcgaggatg cgccggcgga tttcgcggcc
      781 gatcgccgcc gcgcttccca tgagcgtgag actcgaggtg acgtcgagaa acgcctcgtc
      841 cagggatagg ccctccaccc gcggagtgaa ttcgtggaaa atcgcgaaga ctcgttccga
      901 cacctcgcgg tagcgcatca tgcgcggctg cacgcatatc gcctcggggc agcggcgcag
      961 ggcttcacgc atcggcatgg ccgagcgcac gccaaaacgg cgcaccgcgt aactcgccgc
     1021 cgcgaccacg ccgcggccgc cggtgccgcc gacgatcaac ggcttgccct tcagttccgg
     1081 acgatcctgt tcttccacgg acgcgtagaa ggcgtccatg tcgatatgca gaatcgcacg
     1141 ggcgtcgctg cgccgcatcg atcattccac ggtgatggtg attttcttcg aatgcagcgg
     1201 cggatcgaaa gacttgtgca gatagtccgc gaacacgagt tcgagcgtgt gcttgccggg
     1261 cgccaagttg agggtcgtct cggtttgacc cttgccgaaa tgcagcaatt tgtcggtggc
     1321 cggcagcggc tcgtcgagct ttacttcgct caagtccatg tcgaccaaca ggtgatggtg
     1381 tccggtgttg tcgaatttga cgcccgccgg agcgactccc atgcctttca atccgaaacg
     1441 cacggtcacc gggccatgca cggtggcgcc gttgtgcggg gcaatgatat agacctcggc
     1501 attcgcggcg ggcggcgacc ggtcctgcgc cacggcgaaa ccgcagatca aggcaacgcc
     1561 cagcgcgaga agtagacggc tcatttggat ctcctaaagg atggcggacg gctggcccat
     1621 cttacagccg cagcccggca gagacgaata cggatcacaa ggcgtgccca aacctcgatc
     1681 cgggggggct taaggactgc ccgccgccag ctcgtgcggc gcgaaatcgt cgacgtcgag
     1741 aagctgcgat atcttggctt cgtaatcgcg cagaaccgcc gcttccgcct ggttcagaat
     1801 gcccagctgc agggcctggg cgatctgtcc cggcaggtcg agcgcagcga cttgcccgct
     1861 cttgatgccg tctacgcgaa tgcgtttctc caaggattca gccatggtcg ataaaaccaa
     1921 ggcctcttgc agcaacccca agggattgtg cgccgtttgg gtcttataga tatcccgact
     1981 caaacgctcc ctcgctgcgg tcggattcat gatcagatcg gcgatggcgc ttcccaagcg
     2041 gtcgctcggc gcaaaatagg tgaggccgcg cggaaatatc aacaggcgca tgcaagctgc
     2101 cagcggcctc gaggggaaat tgcgcaagaa cagatgcaat tgctcctggg cctgatagag
     2161 gagactgcgg caagcccatt cgaccagcgg caaatcggcg gcttcgcgcc cctgatcttc
     2221 gtagtgcttc agtaccatcg acgccagata catcgccgac aagacgtcgc ccaagcgggc
     2281 cgacaaggct tccttctttt tgaggtagcc gccgagcgtc agcatcgcga catcggccgc
     2341 gagcgcgaac gacgcgctgt agcgattcac gtgctggaag tagcgtgcgg ttgccggcag
     2401 gcgcggcact ttggtgaaac gtgcgaatgt caatgccatg accagcgagc gcacggcgtt
     2461 cgaaatggcg aacccgacgt gacccagaaa cgcgctatcg aattcggcga cgcccgcgtc
     2521 gtgatccgga ttgcgcgccg cattcatttc gcgcaacacg aaggggtggc agcggatcgc
     2581 gccctggccg aaaataatca gactgcgggt caagatgttg gcgccttcga ccgtgatcgc
     2641 gaccggcacg atctggtagc cacgcccgag ataattcttc gggccgaggc aaatcccctt
     2701 gccgccatgc acgtccatgg catcgttggc gatcatgcgg ctgaattcgg tcaggtggta
     2761 cttcaagatg gcggatggca ccgacggttt cgcgccgcgg tcgatcgcgc cggctgtcac
     2821 cgaacgcgcc gcatccatga tgtaggtgcg cgcggccatg cgcgccaaca cttgctcgac
     2881 gccctcgaag cgtccgatgg acatgttgaa ctggcggcgt attcgcgcat aggctgcggt
     2941 cgcataaacg cccgcctggg cgccgccggt ggcgctcgac ggtagagaga tacagcggcc
     3001 caccgacagc tgctcgacca gcatgcgcca tccttggccg gccatcgcga aaccgccgat
     3061 gatggcatcc aacggcacga acacatcatg tccctgaatg ggtccgttct gaaaaggaac
     3121 gttcagcgga aagtgacgcc ggccgatgat gacgccgggc gcagcgcgcg gaatcagggc
     3181 acaggtgatg ccgagatcgg cgcggccgcc catgagcccc tgcgggtcga agaggcgaaa
     3241 cgcaagtccg atcacggtcg cgaccggcgc caacgtgatg tagcgtttcg agaagttgag
     3301 tttgatcccg acgatttgcg cgccctgcca ttcgcctttg cagatgacgc cggtgtcggg
     3361 aatggaggcg gcatcggagc cggcgcgcgg cccggtgagc gcgaagcacg gcacttcctc
     3421 gccgcgggcg aggcgcggca ggaaatggtt cttctgcgct tcggtaccat agtgcagcag
     3481 cagttcggcc ggcccgagtg aatttggcac cgcaatcgtc gaggatgcga cgcccgagcg
     3541 cgatgcgatt ttacagagca cgcaagaatg cgcgtacgcc gaaaattcca atccgccgta
     3601 gcgccgcggg atgatcatcc cgaaaaaacc ttgctccttg atgaaagccc acacgtcggg
     3661 cggcagatcc gcgcggacgt gcgtgatgtc ccagtcatcg agcatggcgc atagatcctc
     3721 gcagggtccc tcgagaaacg cgcgctcggc cgggttgagc gccggcgctt tcgccgacat
     3781 gagcttttgc caatcgggtc cgcccgaaaa caactctccg tcccaccaca ccgttcccgc
     3841 agccagcgcc tcgcgctcgg tcgccgacat cggcggcagc agccggcggt aacttctcaa
     3901 gaatgggcgt gtcagcaggt tgcgacgcag cggccgtagg ttgagcagca gcagcagcag
     3961 gtagggaacc gagacgagca gcttccacca ttcgggcgag cggccgaaca gccaatagga
     4021 aagccacaac aggccgagaa ctgccgaact tcgagccaag ccggcgcggc gataggcgag
     4081 caccagcagc acacccagga atagcgctgc ccaggcaagc aattcgcccg cggccacggc
     4141 caactcattc ataagagtcc gctttagatc aggtctttca caccctcgcg ttcttccagc
     4201 aattcgcgat gcgttgcttg catcttgtcg cggctgaatt cgttgatgct caatccttga
     4261 acgatgctgt actcgccgtt ctggcaggtc accgggtacg aatagatcac cccttcgggt
     4321 attccatagc tgccgtcgga gggtaccgcc atgctgaccc aatcgccggg cggcgtaccc
     4381 agcatccaat cgtggatgtg atcgagcgcc gcggccgccg ccgaggccgc cgatgaggtg
     4441 ccgcgcgcct tgatgatggc ggcgccgcgt tgctgcactg tcgggatgta ggtgtccttg
     4501 aaccactgcg cgtcgaccac cgacagcgcc ggcttgccgg atacggttgc gtgatgcaga
     4561 tcgggatact gggttgccga atgattgccc caaatgatga tctgcttgac cgcggccaag
     4621 ggtgagccgc tcttttcagt cagctgggca agtccgcgat tgtgatccaa gcgtgtcatc
     4681 gccgtgaagc gatgcggcgg caacgacggc gcgttgcgct gcgcgatgag cgaattcgta
     4741 ttggctggat tgcccaccac caatacgcgc acatcgcggc tggcgacggc attgagcgcc
     4801 ttgccctgcg ccgaaaaaat cttggcattc tcgagcagca gatccttgcg ctccatgccc
     4861 gggccgcgcg gccgagcgcc caccagcatg ccgaaattac aatccttgaa ggcgatggcc
     4921 gcgtcgctgg tcgcggttac ccggttgagc gtggggaacg cgcagtcgtt caactccatc
     4981 accacacctt gcaccgctgc gctcgccgcc gggatctcca gcagattcaa attgatcggt
     5041 tggtcggcgc ccaacatctg accggatgcg acgcgaaatg cgagtgcata gccgatattg
     5101 ccggcggcgc ccgtgatggt gaccgtgact ggtttcttca tgacctgctc cgatgcgcaa
     5161 agcgacgaat cttagccgaa atcgcctctc agaagtcggc tcacggccag aattcaggct
     5221 cgccggcgga atagccgggg taggcagcgc ggaagcggcg agactccgca tccgcttcgt
     5281 tgttgcgtcc ggcgcggcgc agcgccgcga tatccgcgag ccatgcggac ggggtgcggg
     5341 tatgcggcgg atcgctgctg tagagccgca gtttgcgatc gagcggtgcg ttcgctgccg
     5401 ctcccgagtg tgctgcgaca cgcaccagat ggggcgcctc gtcggtatgt tttttgggct
     5461 gcggaccgaa tacgatggca aggaccagcg ccatcgacag gaacacgctc gcggcgagcg
     5521 ccagcggggc caggtacggc gagcggatag gtgctgcacc ggaccctctc agcacgtgcc
     5581 ggtcgagcgc cggcggcggt gccggcgccg cctctctctt gtactgacgc gacaatgcgc
     5641 tgtcgccgcg caagtattct tccagatcgt cgggtggaac catgggcgtc acgtgccaac
     5701 cgtccgcgtg cgcagcggcc gcgcaagact ctgcgccggg tgcagcgatt tcttcaattt
     5761 acggatggcg tatcgcagcc ggctcttggc cgtctcgagc ccgacgtcgg tgacgcgcgc
     5821 gatttcctcg atcgtgagtc cggtctcctc gtgcagcaaa aaggcctcgc actgttcacg
     5881 cggcagcgcg ccgagcgccg ccagcaacgc cgattgctgc tcgagaaatt ccgccactgc
     5941 gtcgggacgc tgatactcgg gcacctcagg ttcgggcgct tgagcatcgg caggatcact
     6001 gcgcgctgcg cgccgcatcg cgaggtcgcg gcgaaaaaag tcgatcgtgc agttgtgcgc
     6061 gatgcgaaac aaataggtcg caaattttgc gcgcggctcg tagcggttgc gggtcgcaac
     6121 caatcgcgac cagacctcct ggaataggtc ggcggcggcg gcggcgctgc gcacctggcg
     6181 cagcagatag cgatacagcg gacctttgtg ccgggcgtac agggccaaga atgccgccgc
     6241 gtcgccgtcg cggtagcggg ccatgagccc ggcatcttcg tcgagcgact ccatgctagg
     6301 cagcatacgc gccggcacgg cggcgagcga tcattgtgcg acgcacaatc gcaatcaaaa
     6361 aaatgaacac gaaagtgctt gcatgtacca gtgatgtatg caactatatc accaagtcac
     6421 cgaaacacct cgatgacact taccaggaga acatcatgac cactcagatc tcaaccaaac
     6481 tcgccgctct cgccgtcgcc ctcatggtga acggagtcct cgttggcggg gtcgccttcc
     6541 tgttcaacag ccagctgcag cagcaggtta ccgccctgtc ggtggcccat cgcgccgcgc
     6601 ccgccacctt gggcgcgatt tgatcgccgt ttggaatgaa tgcgttgcct tagactagtg
     6661 tgctaaacag ccacaacagc atatcgagcc aggaatggac cctaattgga acgacaacca
     6721 gccaatctat cgccaactgc gcgatcgaat ggtcgccctg attctcgagg gcgcgctcaa
     6781 ggagggcgat ccgctgccct ccgtgcgggt ggttgccgcc gattaccggt tgaacccgct
     6841 gaccgtgctc aagggctatc aacagctggt cgacgaacat ctggtcgaaa aacgacgcgg
     6901 ccggggaatg tacgtcagcg agggtgcgca acggctgcta ttggccggtg agcgcgaacg
     6961 attcctgaac gaagaatggc cgcagattct ggcggtcatc ttgaggctcg gcttctccgc
     7021 cgaggaattg tttgcgcaaa cgccggcgct gacccacccg gctccgccgc cccgcggcgg
     7081 cttgcctacg gaagaatgag aatcaccatg tctgcgctca tcgaagcctc cgaccttaca
     7141 aaaaaatacg gcgcgcacgt cgcgctcgat catgcgaact tctccgtgca ggcgggccgc
     7201 atcgtcggac tcatcggccc gaacggtgcc ggcaagacca gcgctttgcg cgccatcctg
     7261 gggctcacct cctacgaagg gcagctcaag gttttgggcc gggaaccatt tcgcgagcgt
     7321 accaagctca tggacgaggc ctgcttcatc gccgatgtcg cggtgctgcc ggcatggctc
     7381 aaggtcgacg acgcaatcga cttcgtcgcc ggcgtgcacc ctcgatttcg ccgcgagcgt
     7441 gcggcggcat tgctcgccaa gacccagatc accggcgagc gccgcatgcg cgagctgtcc
     7501 aagggcatga agactcaggt gcatctggct ctgaccatgg ccatagacgc caaattattg
     7561 gtgctcgacg aaccgacgct cggcctcgat attctcgcgc ggcgcgcttt ctacgatgca
     7621 ctggtcaacg attatatgga cgagacgcgg accatactca tcaccacgca ccaggtcgag
     7681 gaaatcgaga atctgttgac cgatgtgctg ttcatcgaca aaggccgcat cgtgctcgat
     7741 tcatcgctcg acgatatcgc gacgcgttac gccgcggttg ccgtggccgc cgacaagagc
     7801 gatgcagcgc gcgccgcagg gccgttctac gagcgccgca tgctcggaaa ggtcgtgctc
     7861 tactacgagc atccggatct cggggaactc gccgaactcg gcgaggtgca cacacccagc
     7921 atctcggatc tatttgtcgc caaactgtcg ggaggtgtgc aatgaatacc accatctggc
     7981 tggtgcgccg cgaattctgg gagaaccgcg cgatctggat cattcctgcg gtgatcggcg
     8041 cgctgctggt gctcgccgcg ttgttcggca aggtgaatat caacttgccg gggccgctgc
     8101 ccgtgcacgc cttcggacag ttgttcatgg tgaccgtcgg cggcacattt tttgtcgtca
     8161 tgtcaatcta cgccacctgg tacctgttgg actgcctgta tgccgatcgc agggaccgga
     8221 gcgtgctgtt ctggaaatcc ttgccgatct cggacacgct gaccgtcctg tccaaattgg
     8281 gcaccgcctt gctcgtcatt cccctggtct attttgcggc ggccgatgcc acgacgctga
     8341 tcatggcctt cattgtttcg gttcgagcgg gctcgaccat cggcgccggc gtctggcacc
     8401 tcgatctgtg ggtgcaactg caggcgttgt ggatctacct gatcgtgacc accgccatct
     8461 ggtacctgcc gattgccgga tacttgttga tcgtgtctgc ctgggcgaaa cgcgccgtga
     8521 tgctctggtc catattgccg cccatcgccc tgctcattgc agaacgatgg ttcgtgggaa
     8581 cgcacgtgac agcgcagctg ctctgggagc gcatggtcgg atgggttccg ctggcattcc
     8641 acatcaatcc gggcgaaggt gtgtgggtga ccagcgtcgt cgacaatgac accattcgca
     8701 cgccgcagag cctgtggagc ctgctcaacg cggccgattt ctttgcaagc ccgcaaacct
     8761 ggctcggcgt tgcggtgggc gcagccttga tcatcggcgc cattcaactg cgcatgcggc
     8821 gcaccgagat ctaacgggcg tgcggcggtc gtattgcccg gccgccgtgc tggcggcgtt
     8881 ctgcgcaagc gctcccggtg cgctggccga ggaatttttc gcgacccgcg accagaatcc
     8941 cctgctacgc ggcttctacc tgccgttgcc gagcgacgcg cggcaggatg ccgatgcggc
     9001 cctgtcggca acactgctgc tcagcaatac gctcaacgtc gaaagcaacg cgcacgagag
     9061 cctgttcgtc gacggcgaaa gcctcgcact cgatcttgcc tacgagagtg ctctggcgcc
     9121 gggttggcgc tatcgtttca gcgtgccgct cgtccacgat agcggcggcg tgctggattc
     9181 ggtcattgat acctggcatg aggtcttcgg ctttccccgc ggcaaccgtc ccttctaccc
     9241 caagcgccag atcgattact tctacgcggg cgtgggcaag atcgacctcg atcacccgca
     9301 atcgagcatc ggcgacctcg cggccgacct gggctggtac gccgtcgacg atgagcgccg
     9361 gaccgtatcg gtatggggag gcctcaaggc ccccaccgga tccgtggccg atctcaccag
     9421 cgacggcgcc tgggacagcg cgctgtgggc ccatgccgcc ctgcgttggc caaagtggca
     9481 gttcgcggcc gagctcggcg tcgcgcagcc gttcggcgat gaactttttg ccggaaaggc
     9541 gcaccgcagc tcggaattcg cgaggcttgc ggccagccgc gcgctcggag cgacatggtc
     9601 gctgcgcgcg caactcgatg gccagtcggc gcgcgtggcg ggcagcgatc tacgctttct
     9661 cggccccagc ctgcagttga cggtcggcgc cgtgcggcgc ctctccggcc ggtggcgcat
     9721 gcaaatggga ttcgccgagg atgttgccgt gaacacggca cccgacatca ccttcttcct
     9781 tgggattcac gactaagcag tgcaaaatag ccgcccaagg taaattccat tttgtaggag
     9841 agccgatgta caagacgaag atcgatcttt cggaaaagac ccgccgcaat gtgatcgcca
     9901 tactcaacga tcgactcgca gatgcgatcg atttgcaaag ccaggtcaaa caggcgcatt
     9961 ggaacgtaaa aggtcccaac ttcatcgccc tgcacgagct gttcgataag atctccgatt
    10021 tgacgttgga acaaattgac gaaatcgccg aacgcgtgac gagcctgggc ggtacggccg
    10081 agggcacggt ggcggtggcc gccaagcgca gcaaattgaa gaactatccg ctctccatca
    10141 cagccggcaa agaccatctg ttctatttgt cgacccaatt gtcggtgttc ggcaaagccg
    10201 tacgcacggc catcgaggac acggacaaac tgggcgatgc ggacaccgcg gatctattca
    10261 cgggaatttc ccgcgacctc gacaagtacc tgtggtttct ggaagcgcat ttgcaagaga
    10321 aagcctgatg gccgatcccg gcatgagcaa ctcttccgag ccgggcctcg cttcggcgct
    10381 gaccaccttg cgcggcctgc tcactgccgg cgagaccttg gaggcctggg ccgtgcagca
    10441 ccgcctgtac gcgctgacgc accggcgcgc ctgcatcgcc gcaaccagcg gccgcttcat
    10501 ttctctcaat cgccgattgc tcggcggcta cgagtcggcg gacattcgct ggcaggacct
    10561 caaagaaacc cgcatcagcg cgggcattct cgcggccgat ctgaccttgg tcgcccagaa
    10621 cagcgccgac ctcaacatcg gatcggaggt caatcgcgta tggaccttca gcgggctgcg
    10681 caaggatcag gcgcaggcga tgtaccggat ttgccagcag cacgaccagg tgtggcgcga
    10741 gaagcggcgc attcgagagc tcgaggaact acgcgcaaaa tccggcggcg tgcagatcgg
    10801 cggcggccaa gctgcctatg cc
//
LOCUS       DASWZT010000283        13244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_31393, whole genome shotgun sequence.
ACCESSION   DASWZT010000283 DASWZT010000000
VERSION     DASWZT010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13244
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31393"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VGE92_07670"
     CDS             complement(<1..320)
                     /locus_tag="VGE92_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY1313738.1"
                     /translation="MATTTEVKVPDIGDFKNVPVIEVLVKVGDTVKAEDPLVTLESDK
                     ATMEVPSPDGGTVQELHVKVGDKVAEGSVILTLATAGASATTAASPAASNVAAAVAAA
                     AAAP"
     gene            complement(332..2992)
                     /gene="aceE"
                     /locus_tag="VGE92_07675"
     CDS             complement(332..2992)
                     /gene="aceE"
                     /locus_tag="VGE92_07675"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEY1313739.1"
                     /translation="MRMDVSKDVDPLETREWLDALRSVQQNRGADRVDFLMSQLLEAA
                     RRVGARGIASLNTAYCNTIAPEREAKSPGNRDIEHKIRSIIRWNAVAIILKANKQSSE
                     LGGHIASFQSSALLYDIGYGHFWHAPTEQHGGDLIYVQGHVAPGIYARAFLEGRLSET
                     QLLNYRQETEGKGISSYPHPWLMPDFWQFPTVSMGLGPLMAIYQARFLKYLEGRGLAK
                     TEQRKVWAFLGDGECDEPESLGAISLAGREQLDNLIFVINCNLQRLDGPVRGNGKIIQ
                     ELEAVFHGAGWNVIKVVWGSGWDALLAKDTGGFLLKRMQECLDGEYQDFKSKNGAYVR
                     AHFFNSPELQALVAHLSDEEVWNLNRGGHDPVKVFAAYAAAVAHKGQPTLILAKTVKG
                     YGMGESGEGQMIAHQAKKMTEDALKQFRDRFQVPLSDAELLDLPFVRLPEGSEEMQYL
                     RAHREALGGSLPQRRRKSAPLEIPPLEQFDRLLKDSGERENSTTMAFVQMLGTLVRDK
                     AIGRHIVPIVPDESRTFGMEGMFRQLGIYSAVGQLYKPQDADQLMYYRESKDGQVLQE
                     GINEAGAMSSWIAAATSYSSNDVPMIPFYIYYSMFGLQRVGDLAWAAGDMRCRGFLLG
                     GTAGRTTLNGEGLQHEDGHSHVLASTIPNCISYDPTFAYEVVVIIREGMRRMYQAQED
                     VYYYLTLMNENYPQPGMPPGAQDGIIKGMYLFKDAGKTSGPRVQLFGSGTILREVLAA
                     ADLLKSDFQVSADVWSVTSFNELRRDGMSAERWNLLRPDAPRRKSYLETCLEDRSGPV
                     IASTDYMRVFADQVRNQISRRYVTLGTDGFGRSDYRAKLRQFFEVNRHYVVLAALKAL
                     ADDGEIKPGQVMQAIKKYGLDTERPDPWLV"
     gene            complement(3026..4204)
                     /locus_tag="VGE92_07680"
     CDS             complement(3026..4204)
                     /locus_tag="VGE92_07680"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY1313740.1"
                     /translation="MEDVVIVAAGRTAVGKFGGTLAKIAAADLGAHVLKALLTRTGLK
                     GDQIDEVILGQVLTAGCGQNPARQASIRAGIHNMVPAMTLGKVCGSGLKATHLAAQAI
                     KCGDAEIVIAGGQENMSASPHILPNSRDGYRMGDAKIVDSMIVDGLWDAFNQYHMGTT
                     AENVAKKYEISRREQDEFAAASQSKAEAAQKAGRFKDEIIALEIPQRKGPPVSFDTDE
                     YVKAGTTVEALSVLKPAFSKDGTVTAGNASGINDGAAAVVMMSAKKARELGLTPLVTI
                     KAYSSAGVDPSIMGMGPVPATQLCLKKAGWSAKDLDLMEINEAFAAQAIAVNKEIGWD
                     TNKINVNGGAIALGHPIGASGARILVTLIHEMLKRDAKRGLASLCIGGGMGVALAVER
                     "
     gene            complement(4235..4687)
                     /locus_tag="VGE92_07685"
     CDS             complement(4235..4687)
                     /locus_tag="VGE92_07685"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003806.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEY1313741.1"
                     /translation="MTTRIYLLNGPNLNLLGERQPQIYGSATLAQLESQCRDVAQRCH
                     FELLARQTNAEHEMIGWLHEARRSGGIVINPAAFSYHSVPVLDALLMCECPVIEVHIS
                     NIHRREEKWRANSILSAATTGTITGLGTDGYRLAIEHLYYLNSKGSVT"
     gene            complement(4899..7019)
                     /locus_tag="VGE92_07690"
     CDS             complement(4899..7019)
                     /locus_tag="VGE92_07690"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEY1313742.1"
                     /translation="MSDRSVAGPGVRAIERLLKPRSVAVVGASPTPGALGASVVANLE
                     RMKFSGDIHLINPKRDAIGDRRCLKSVDDLPLGVDVAVLAIPRSAVLGTVKALIGRQA
                     GAAIIFSAGFAEGGDSGLAEQRELARLAADAGMVIEGPNCLGLVNYVDGIALTFVETP
                     AIALGSRPGIGIVSQSGAMAAVVGVTLTAKNLGVSYSISTGNEAASGVEDYLEYLLGD
                     RHTQVVALIVEQFRQPRRFLTLAGRALAAGKALVLLHPGRSSAARESAATHTGAMAGD
                     YVVMRKLVERAGVVVADTLEEFADILELTLRCGAAPAAGAAVLTESGAFKALTLDFCE
                     QIGLDLPTLTNDSAPQLRAALPEFVPVSNPVDLTAQALVDPDLYGRTLAALLQDARVG
                     SIILGIIQTDAKTAALKFPPIIRAIAQLKPQKPVIFAGLDDGAYVPAEYIESLRALNV
                     PYFASPERAYRAVARWCASAGRDSVADAAAVILSRPLPSGVLAEYQSKEILRSIGLAF
                     PAGRLVRTLEEAQRAALDLGMPVVLKAQSADLPHKSDAGGVILGIKDAPALEQGWQRL
                     HANIARTRPALVLDGVLIESMSKKGVELIVGGRNDPEWGAITLAGFGGVQAEILQDVR
                     LLPADLTVEGIERELNLLKSAAILRGFRGSPALDVAAAADIISRVGALLRAQPSIREI
                     DLNPVIIYPRGEGALALDALIVIS"
     gene            complement(7016..7891)
                     /locus_tag="VGE92_07695"
     CDS             complement(7016..7891)
                     /locus_tag="VGE92_07695"
                     /EC_number="4.1.2.61"
                     /inference="COORDINATES: protein motif:HMM:NF006588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxycinnamoyl CoA hydratase/lyase"
                     /protein_id="HEY1313743.1"
                     /translation="MNTANTTEAAAPERAEADTVAFTVIDQIAWVKFNRPEKRNCMSP
                     KLNRQMLRVLDQLEFSKDVGVLVLSGEGTAWSAGMDLKEYFRETEAQGLGATRAAQRD
                     AYGWWRRLRWYQKPTIAMINGWCFGGGYGPLFGCDLAFAAEEAQFGLSEINWGILPGG
                     GATKVATELLSFRRAMYHSLMGENIDGRTAAEWGLINEALPLAKLRERVESVARTLLK
                     KNPVALKATKDAIRRVGDMTYDNAEDFLIRAQEAANSYDNEGRKEGIKQFIDDKTYKP
                     GLGAYDIAKQSPKKK"
     gene            complement(7910..9307)
                     /locus_tag="VGE92_07700"
     CDS             complement(7910..9307)
                     /locus_tag="VGE92_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEY1313744.1"
                     /translation="MEFERISPITNETASRAQAMTPAEACGVADRAAAGFAGWSALGP
                     NARRSLLMNAAAALEKRKDDFVAAMMGETGSTAGWAMFNLMLAVGMVREAAALTTQIG
                     GEVIPSDKPGCLALALREPVGVILGIAPWNAPIILGVRAIAVPLACGNSVILKASELC
                     PQTHTLIIEAFAGAGFPDGAVNIVTNAPQDAAAVVGALIDHKAVKRINFTGSTAVGKI
                     IAKRAAEHLKPCLLELGGKAPLIVLDDADLDEAVKAAAFGAFMNQGQICMSTERIIVV
                     ESVAAEFVKRFAAKAKSMAVGDPRQGKTPLGAVVDRKTVSHVNALIDDAKSKGATLAA
                     GGTADSVLMAATVIDGVTSAMNIYRDESFGPVVGIIRAKDEADAIRLANDSEYGLSAA
                     VFTRDTARGLRVARQIRSGICHINGPTVHDEPQMPFGGVGASGYGRFGGKAGIDQFTE
                     LRWITIETQAGHFPI"
     gene            complement(9364..9870)
                     /locus_tag="VGE92_07705"
     CDS             complement(9364..9870)
                     /locus_tag="VGE92_07705"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY1313745.1"
                     /translation="MTIDPLRDFPGYALRRASIAVMVELARRISGLGLRPTEATVLVV
                     IEANPGITQSEVGQLLDIARANMAPLTARLFERDLIERERVDGRSQGLSLSATGRQLT
                     RKVLQAMSENEAALLAKIPRAHHEIFVNALHALWDSQKIDGGAFRQFRLQKPAKRSTP
                     KARSARDQ"
     gene            10045..11400
                     /locus_tag="VGE92_07710"
     CDS             10045..11400
                     /locus_tag="VGE92_07710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1313746.1"
                     /translation="MDTPVDKISQLEADAAALRAQNAELRRQLQLRNHALDSTPIFFL
                     ISALTDPECVVYCNKAVADQLGLPRDEIIGRPLSLLTQWVGRNPTYRADVSAALRAGH
                     TFRYEDEVRRSDGTSFWLGVSTMPLHDARGRLTHAVTVGADITAKRDEALKKQALQDK
                     LVEEMRRRELMGNELQVAQKLESVGRLAAGIAHEINTPTQFIGDNVSFVKEAVTEMFA
                     VIDRIADALEPGSDGTVRGATLAALLAGVDMEYLHAEVPRAIEQSLEGIARIQTIVGA
                     MKDFSHPALDRTPIDINRAIGSTVTVATNEWKYVAEVKTEFDLDLPSVPVMPGMFNQV
                     ILNLIVNAAHAIAAVAKSRPHKGTITVSTRQVERWAEVRVSDTGCGIPEAIRDRIFDP
                     FFTTKPVGKGTGQGLAIAHDAIINKHRGAISVESEVGVGTTFIVRLPLDLDNDEITNA
                     A"
     gene            11518..12738
                     /locus_tag="VGE92_07715"
     CDS             11518..12738
                     /locus_tag="VGE92_07715"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1313747.1"
                     /translation="MNSVNLAPGRGKVRIAFVDDESSILQGMRRTLHGMRNEWEMEFF
                     GSGEAALASLASSAADVIVSDMRMPGMDGWEFLAEVKKRHPQSVRLLLSGYADPSSIM
                     RSVGTAHQYMAKPCDSATIKAAIVQTQMLRSLLSSPRLALLVGHVELLPSTPKAFQEI
                     LACIRRPDATLVDAAQIIARDVAMTANILKLVNSAFFGSRQSITRIDRAVAYLGMDTL
                     GSLVLAHGVFQSGVSIGIEGFSLEELWRHSLQTALLSKAIALDRGLSAINVEEAFLVG
                     LLHDVGKIVFATRAPAVGDSAISNEEAVAQMQSHHAEVGAYLLGLWGFPNSIVEAVAF
                     HHNPSQTADSSLGLAGIIHIADRLSRADGAIGATPADIGIEAGYLERHGVLNKFADWK
                     AAAAGGLEPQRAAS"
     gene            12735..>13244
                     /locus_tag="VGE92_07720"
     CDS             12735..>13244
                     /locus_tag="VGE92_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1313748.1"
                     /translation="MSDRMSDATSTATILLVDDEINVTEALKRSFRREPYTFVTATSG
                     EAALQVLASRHVDVVVSDEQMPGMSGSEFLATVRALYPHTIRMILSGQASLEAAVRAI
                     NEGEVYRFFLKPCNPTDLLFTIQRALAHKQLEEQSRRLLREFQKQAAILDKLKHTGED
                     LLRLDTDDQG"
BASE COUNT         2489 a         4093 c         4051 g         2611 t
ORIGIN      
        1 tccggagcgg cagcggcagc ggcgacggca gccgcgacgt tggaggcggc gggggaggcg
       61 gcggtcgttg ccgaggcgcc ggcggtcgcg agcgtcagaa tgacgctgcc ctcggcgacc
      121 ttgtcaccga ccttgacatg gagttcttgg acggtgccgc cgtccggcga gggcacctcc
      181 atggtcgcct tgtcggactc caatgtcacg agcggatcct ccgccttgac cgtgtcgccc
      241 accttgacca atacctcgat caccggcacg tttttgaagt cgccgatgtc cggaactttg
      301 acttccgtcg ttgtcgccat ggctcgctcc gtcataccaa ccagggatcg gggcgctcgg
      361 tgtcgaggcc gtatttcttg atggcttgca tgacctggcc gggtttgatc tcgccgtcat
      421 cggccaatgc tttcaacgcc gcgagcacca cgtaatggcg gttgacttcg aagaactggc
      481 gcagtttcgc gcggtagtcg ctgcgcccga agccatcggt accgagcgtc acgtagcgtc
      541 tcgatatttg attgcgcacc tgatcggcga agactcgcat gtaatcggtc gaagcgatca
      601 ccggaccgct gcgatcctcc aaacatgttt cgaggtagct cttgcgccgc ggcgcgtccg
      661 ggcgcagcaa attccagcgc tcggcgctca tcccatcgcg tcgcaattcg ttgaagctgg
      721 tcacgctcca cacatccgcg ctcacttgga agtcgctctt caacagatcc gccgccgcga
      781 gaacttcccg caaaatggtg ccgcttccga acagctgtac acgcggcccg cttgtcttgc
      841 ccgcgtcctt gaacaagtac atgcccttga tgatgccgtc ctgcgccccg ggcggcatgc
      901 cgggttgcgg gtaattctcg ttcatcagcg tcaagtagta gtagacgtcc tcttgagcct
      961 gatacatgcg ccgcatgcct tcgcgaatga tcaccacgac ttcgtaagcg aaggtcggat
     1021 cgtaggagat gcagttggga atcgtcgacg cgagcacatg gctgtgtcca tcttcgtgct
     1081 gtagtccttc gccgttcaag gtcgtgcggc cggcggttcc ccccagcagg aagccgcggc
     1141 agcgcatgtc gccggcggcc catgcgagat cgccgacgcg ttgcaggccg aacatcgaat
     1201 agtaaatata aaatggaatc atcggcacgt cattgctgct gtacgaggtc gcggcagcga
     1261 tccaggatga catcgcaccc gcctcgttga tgccttcctg cagcacttgt ccatccttgg
     1321 attcgcggta atacatgagt tgatcggcat cctgcggctt gtacagctgg cccaccgcgg
     1381 agtaaatgcc aagctggcgg aacatgccct ccataccgaa agtgcgcgac tcatcgggca
     1441 cgatcggcac gatgtgcctg ccgatcgcct tgtcgcgtac cagcgtgccg agcatttgca
     1501 cgaaagccat ggtggtcgag ttctcgcgct cgccggaatc cttgagcagg cgatcgaact
     1561 gctccagggg cgggatttcc aaaggcgcgg attttcgccg gcgttgcggc aaggaaccgc
     1621 cgagcgcctc gcgatgggcg cgcaggtatt gcatctcctc gctgccttcc ggcagtctga
     1681 cgaacggcag gtcgagcagc tcggcatcgc tcagcggcac ttggaatcgg tcgcgaaact
     1741 gtttcaaggc atcctcggtc attttcttcg cctggtgcgc gatcatctgg ccttcacccg
     1801 attcgcccat gccgtagccc ttgaccgttt ttgcgagaat cagcgtcggc tgtcccttgt
     1861 gcgcgaccgc cgctgcatag gcggcaaata ccttgacggg gtcgtgaccg ccgcgattca
     1921 aattccacac ctcctcgtcg cttaagtgcg ccaccaacgc ctgcagttcg ggagagttga
     1981 agaaatgcgc gcgcacgtaa gcgccgttct tcgacttgaa gtcctgatac tcgccgtcga
     2041 ggcactcctg catccgcttg agcaagaagc cgcccgtatc cttggcgagc agagcgtccc
     2101 agccggagcc ccatacgacc ttgatgacat tccagccggc accgtggaat acggcctcca
     2161 gctcctggat gatcttgccg ttgccgcgca ccgggccgtc gagacgctgc aggttgcagt
     2221 tgatgacgaa aatcaggtta tcgagctgtt cccggccggc gagcgagatc gcgccgagcg
     2281 attcgggctc gtcgcattcg ccatcgccta agaacgccca cactttgcgc tgttcagtct
     2341 tggccaagcc gcgtccttcc aagtacttga ggaatcgcgc ctgataaatg gccatcaacg
     2401 gcccgagtcc catcgacacg gtgggaaact gccagaagtc aggcatcagc cacgggtgcg
     2461 gataggagga gatgccctta ccctccgttt cctggcgata gttgagcagc tgcgtttcgg
     2521 aaagacggcc ctcgaggaat gctcgagcgt agatccccgg ggcgacatgt ccttggacat
     2581 agataagatc gccgccgtgc tgctcggtgg gcgcatgcca gaagtggccg tagccaatgt
     2641 cgtagagcag cgccgaagac tgaaagctcg cgatatggcc gcccagctcc gaggattgct
     2701 tgttggcctt gaggatgatt gcgaccgcgt tccaacgaat gatcgagcgg atcttgtgtt
     2761 cgatgtcgcg attaccgggg gatttcgctt ctcgctccgg cgcaatggta ttgcagtacg
     2821 ccgtgttgag cgaggcgatt ccgcgggcgc ctacgcgacg cgccgcctcc agaagctggc
     2881 tcatcaggaa atcgacccga tcggcgcccc ggttttgctg caccgagcgc aaagcgtcaa
     2941 gccattcccg agtctccagc ggatccacgt ccttggaaac gtccatgcgc atctcctgtt
     3001 accctcagcc gggcggcgga tcttcttagc gttcgacggc gagcgcgacg cccattccac
     3061 ccccaatgca gagtgacgcg aggccacgtt ttgcgtcgcg cttcagcatc tcgtgaatca
     3121 aggtcacgag gatcctcgcc ccggaggcgc caatcgggtg gcctaaggcg attgcgccgc
     3181 cattcacgtt gatcttgttc gtgtcccagc cgatctcttt gttgaccgcg atcgcctgcg
     3241 cggcgaacgc ctcattgatc tccatgaggt ccaaatcctt ggccgaccaa cccgctttct
     3301 tcagacacag ctgagtcgcg ggcaccggtc ccattcccat gatgctcgga tcgaccccgg
     3361 ccgaggagta cgccttgatg gtgaccagcg gcgtgagccc gagctcccgg gctttcttgg
     3421 cggacatcat gaccacggcg gcggcgccgt cgttgatgcc ggaggcgttg cccgcggtga
     3481 ccgtcccgtc cttcgaaaac gccggcttta aaaccgacag cgcctcgacg gtcgtgcccg
     3541 ccttgacgta ctcgtcggtg tcgaagctca cgggcggacc cttgcgctgc ggaatttcga
     3601 gcgcaatgat ctcatccttg aatcggcccg ctttctgtgc ggcctcggct ttggattgcg
     3661 aggcagcggc gaattcgtcc tgttcacgac gcgagatctc atactttttg gcgacgttct
     3721 cggccgtcgt gcccatgtgg tattgattga acgcatccca caagccatcg acgatcatgc
     3781 tatcgacgat ctttgcgtcg cccatgcgat agccatcgcg cgaattgggc agaatgtggg
     3841 gcgaggcgct catgttctct tggcccccgg cgatgacgat ctcggcatcg ccgcacttga
     3901 ttgcctgcgc ggcgagatgg gtcgccttga gtcccgatcc gcacacctta ccgagtgtca
     3961 tcgccggtac catattgtga atccccgcgc ggatggaggc ctgccgcgcg ggattctgac
     4021 cgcacccggc ggtaaggacc tggccgagga tgacctcgtc gatttggtcg cccttgaggc
     4081 cggtgcgggt caacagcgcc ttcagtacat gggcgcccaa gtcggcggcg gcaatctttg
     4141 ccagagtacc gccgaatttg ccaacggccg tgcggcccgc ggctacgata acgacatctt
     4201 ccatgttcat atcctcttca agcgctgttc gcggttaggt tacggagcct ttcgaattca
     4261 aatagtacaa gtgctcgatc gccaatcgat agccgtcggt gccgaggccg gtgatggtac
     4321 cggtggtggc ggcgctcaaa atcgagttcg ctcgccattt ctcctcgcgg cgatggatat
     4381 tgctgatgtg aacttcaatg acagggcact cacacatgag cagggcgtcg agtaccggca
     4441 ccgagtgata ggaaaaagcg gctgggttga tgacgatgcc gcccgagcgg cgcgcctcgt
     4501 gcagccagcc gatcatctcg tgctccgcat tcgtttgacg ggcgagaagt tcgaaatgac
     4561 agcgctgcgc gacgtcccgg cattgcgact ccaactgcgc caaggtcgcg cttccataga
     4621 tctgcggttg gcgctcgccc agcaagttca gattcggacc attcaacaga taaatgcgcg
     4681 tggtcatgac gaggcgacag aggctgccat tgctgcggga cactccaatg aatctagtgt
     4741 ggtctgcggc gcgagattcg cccggttagt atagcaaatc aggccgcacg ggcgtgggaa
     4801 gggcactcgg tgacggagcc gcgctcgggc cggtgatcgg cgggcggctc gtgggagcgc
     4861 atgtgcccat ggcgctggtc tgccggccgc gatgacggtc aggatatgac gatcagtgca
     4921 tcgagagcga gtgcgccttc gccgcgcgga tagatgatca ccgggttgag atcgatttcc
     4981 cggatcgagg gctgcgcgcg cagcaaagcg ccgactcgcg agatgatgtc ggcggctgcc
     5041 gccacatcga gtgccggcga gccgcgaaat ccgcgcaata tcgcggcgct cttcagcaga
     5101 ttcagttcgc gctcaattcc ctcgaccgtc aggtcggccg gtagcaggcg cacgtcctgc
     5161 agaatttccg cctgcacgcc gccaaaaccg gcgagggtga ttgcacccca ttccggatcg
     5221 ttgcgcccgc cgacgatcaa ctcgacgccc tttttgctca tgctttcgat caacacaccg
     5281 tcgagcacca aggcgggacg ggtgcgggcg atattggcgt gcagacgctg ccagccttgc
     5341 tcgagggcgg gagcgtcttt aatccccagt atcacgccgc ctgcgtcgct cttgtgcggc
     5401 aaatccgccg actgtgcctt gagcaccacc ggcatcccca aatctagagc ggcccgctga
     5461 gcttcctcca acgtgcgcac cagacgtccc gccggaaaag cgagcccgat cgacctgagg
     5521 atctccttgg actgatattc ggcgagaacg cctgagggaa gcgggcggct caggatcact
     5581 gctgcggcgt ccgccacgga atcacgcccg gccgacgcgc accagcgcgc gaccgcgcgg
     5641 taggcgcgct cgggggacgc gaagtagggc acgttgaggg cgcgcaagct ctcgatgtac
     5701 tctgcgggaa cgtaggcgcc gtcatcgagt ccggcaaaaa tcaccggctt ttgcggcttg
     5761 agctgcgcaa ttgcccgaat gatgggcggg aatttgagcg ccgctgtctt ggcgtcggtc
     5821 tgaatgatac cgaggatgat gctgcccacg cgtgcatcct gcagcagcgc tgccagggtg
     5881 cggccgtaaa gatcgggatc gaccaatgcc tgcgcagtca agtcgacggg attgctcacc
     5941 ggaacaaact ccggcaacgc ggcgcgcagc tgcggcgcag agtcattggt caatgtcggc
     6001 agatcgaggc cgatctgttc gcaaaaatcc agcgtaagcg ccttgaatgc accggactcg
     6061 gtgagtaccg cggcacccgc ggcaggcgcg gcaccgcagc gcagggtcag ctccagaata
     6121 tccgcgaatt cctccagcgt atcggcgacc acaacgcccg ctcgttccac gagcttgcgc
     6181 atcacgacgt agtcgccggc catggcaccg gtgtgcgtgg cggccgactc gcgcgccgca
     6241 ctgctgcggc cgggatgcaa cagcacaagc gccttacccg cggcgagcgc gcggcctgcc
     6301 agagtcagaa atcgccgcgg ctgacgaaat tgttcgacga tcaatgcgac gacctgcgtg
     6361 tgccgatcgc cgagcaagta ctccaggtaa tcctcgacgc cgcttgcggc ttcattgccg
     6421 gtggaaatcg aatacgacac gcccaggttc ttggccgtga gcgtcacccc cacgacagcg
     6481 gccatggcgc cgctttgcga gacgatgccg atgccgggac gagaaccgag cgcgatggcg
     6541 ggcgtctcta cgaaggtcaa cgcgatcccg tcgacgtaat tgaccaagcc caggcaattc
     6601 ggaccctcga tgaccattcc agcatccgcc gcgaggcgcg caagttcgcg ttgttcggca
     6661 agcccgctgt cgccgccttc ggcaaatccg gccgagaaga tgatggccgc gccggcttgg
     6721 cgcccaatga gcgccttgac cgtgcccagc accgcgctgc gcggaatggc gagcaccgcg
     6781 acgtccacac cgagcggcag gtcatcgacg gatttcaagc agcgacgatc gccgatcgcg
     6841 tctcgcttgg gattgatgag atgaatgtcg cccgagaatt tcatgcgctc gagattcgcg
     6901 accaccgacg caccgagcgc gccgggggtg ggcgatgcgc cgaccacggc caccgagcgc
     6961 ggtttcagca atcgttcgat tgcgcggacg ccggggcccg cgacgctgcg gtcgctcatt
     7021 ttttctttgg gctttgcttc gcgatgtcgt aagcacccag cccgggttta taggtcttgt
     7081 cgtcgatgaa ctgcttgatg ccttccttgc ggccttcgtt gtcgtaggag ttcgcagctt
     7141 cctgcgcgcg gatcagaaaa tcttccgcat tatcgtaggt catgtcgccg actcgccgga
     7201 tcgcgtcctt ggtggctttc agcgccaccg gatttttctt cagcagcgtg cgtgccacgc
     7261 tttccacccg ctcgcgcaat ttagcgagcg gcagcgcttc gttgatcaag ccccactcgg
     7321 cggcggtgcg gccgtcgatg ttctcgccca tcaatgagtg atacatggcg cggcgaaatg
     7381 acagcagttc cgttgccacc ttggtggccc cgccgcccgg caaaattccc caattgattt
     7441 ccgagagtcc gaactgcgct tcttcggccg cgaacgcgag atcgcagcca aacaagggtc
     7501 cgtagccgcc gccgaagcac caaccattga tcatggcgat cgtcggtttt tgataccacc
     7561 gcagtcggcg ccaccaaccg taggcatcgc gctgtgcagc gcgcgtcgcg ccgaggcctt
     7621 gcgcttccgt ctcgcggaag tattccttaa gatccattcc ggccgaccaa gcggtgcctt
     7681 ctccggacaa caccagtacc ccgacgtcct tgctgaactc caattggtcg agcacccgca
     7741 gcatctgccg attgagcttc ggactcatgc aattgcgttt ttccgggcgg ttgaacttca
     7801 cccaggcaat ctgatcgatg acggtgaagg caacggtgtc tgcctcggcg cgttccggcg
     7861 ctgcagcttc ggtcgtgttg gcggtgttca tcgcaagtcc tttacaagtt taaatgggga
     7921 aatggcccgc ttgggtttcg atggttatcc agcgcagttc agtaaattgg tcgataccgg
     7981 ccttgccgcc gaagcggccg tatccggacg cgcccacccc gccaaaaggc atctgcggtt
     8041 catcgtgcac ggtggggcca ttgatgtggc agatgccgga acggatctgg cgggcgacgc
     8101 gcaggccgcg cgccgtgtcg cgggtgaaga cggctgccga caatccgtat tcgctgtcgt
     8161 tggccagccg aatggcatcc gcttcgtctt tggcgcgaat aattccgacc accggaccga
     8221 agctttcgtc gcgatagata ttcatggcgc tggtgacacc gtcgatgacg gttgccgcca
     8281 tgagtacact gtcggcggtt ccgcctgcgg caagcgtcgc ccccttggat ttcgcatcgt
     8341 cgatcagggc attgacatgg ctgaccgtct tgcgatcgac cacggcgccc aacggcgtct
     8401 tgccttggcg tggatcgccg accgccatgg atttcgcctt cgcggcaaag cgcttgacga
     8461 attccgcggc aaccgattcg accacaatga tccgctcggt tgacatgcag atctggccct
     8521 gattcatgaa ggccccgaaa gccgccgctt tcacggcttc gtcgagatcg gcgtcgtcca
     8581 agacaatcaa cggcgccttg ccgccaagct ccaaaaggca aggcttcagg tgttcggcgg
     8641 cgcgcttcgc aatgatcttg ccgaccgcgg tggagccggt gaaattgatg cgcttcacag
     8701 ccttgtggtc gatcagcgcg ccgaccacgg cggcggcatc ttgcggtgca ttggtgacga
     8761 tgttcaccgc tccatcgggg aatccggcgc cggcaaaagc ctcgatgatc agcgtatgcg
     8821 tctgcggaca cagctccgag gctttgagga tcacggaatt accgcaggca agcggaaccg
     8881 cgatcgcgcg cacgccgaga atgatcggcg cgttccaagg cgcgatgccg agaatgacgc
     8941 cgacaggctc gcgcagagcc aaggccaggc aacccggttt gtcggagggg atgacctcgc
     9001 cgccgatctg agtcgtgagg gccgctgctt cgcgcaccat gccgacggcg agcatcaaat
     9061 tgaacatcgc ccaaccggca gtcgagccgg tctcgcccat catggcggcg acgaaatcgt
     9121 ctttgcgctt ctccagcgcc gcagccgcgt tcatgagcag cgaacggcgc gcgttcggcc
     9181 ccagtgccga ccatccggca aagcccgcgg ccgcgcgatc agctacgccg catgcttccg
     9241 ccggcgtcat cgcctgggca cggctcgcgg tttcgtttgt gatcggactg atgcgttcga
     9301 attccatgag aacccccgtg tattgtcaga acactataat cgattgctat atatcatagc
     9361 aatctattgg tcccgcgccg agcgcgcttt tggcgtggat cgcttggcgg gtttttgcag
     9421 cctgaattgg cgaaatgcgc cgccatcgat cttctgcgag tcccacaagg cgtgcagcgc
     9481 gttgacgaaa atttcgtggt gagcccgcgg tatcttggcg agcagggcgg cttcgttctc
     9541 cgacatggcc tgcagcactt tcctcgtgag ttggcgcccg gtcgccgaaa ggctgaggcc
     9601 ctgagatcgg ccgtcgaccc gttcacgttc gatcaggtcg cgctcgaaga ggcgcgcggt
     9661 gagcggcgcc atattggccc gcgcaatatc caaaagctgg ccgacctcgc tctgtgtgat
     9721 tcccgggttg gcctcgatga ccacaagaac cgttgcttcc gtcggccgca ggccgaggcc
     9781 cgagatgcgc cgcgccaatt ccaccatgac ggcgatcgag gctcgacgca gcgcatatcc
     9841 agggaaatcc ctaagaggat caatcgtcat tcttcgcctc ccggccgcga aaaaaatcgc
     9901 cataacgatt gcgagtgccc atgatcgcta tattatatag cgatgattcg tgcggacgca
     9961 accgtccgtt gccctgccaa tccgcggtcg ccctgacgca cccgccggct aaagactggt
    10021 tcattccagc cgatagagcc gaacatggat acacctgtcg ataagatctc gcagctcgaa
    10081 gccgacgcgg cggcactgcg tgcgcaaaat gccgaattgc ggcgtcaatt gcagctgcgt
    10141 aatcacgcgc tcgactcgac tcccatattt ttcctgatct cggcgctcac cgacccggaa
    10201 tgcgtcgtgt actgcaacaa agccgtggca gaccaattgg gcctgcctcg ggatgagatc
    10261 atcggccggc cgctgagtct tctcacccaa tgggtgggtc gcaaccctac ctaccgagcg
    10321 gatgtatcgg ccgcattgcg tgccggtcat actttccgtt acgaagacga agtgcgtcgc
    10381 agtgacggca ccagcttttg gctcggcgta tcgacgatgc ccttgcacga cgcgcgcggc
    10441 cgcttgaccc atgccgtgac cgtgggtgcg gatatcactg ccaagcgcga tgaggcactc
    10501 aagaagcaag ccttgcaaga caagctggtg gaagaaatgc gtcggcgaga actcatgggc
    10561 aacgagctgc aggttgcgca gaagctcgag tccgtgggcc ggctcgcggc cggcattgcc
    10621 cacgagatca atacgccgac ccaattcatc ggcgataacg tcagtttcgt gaaggaagca
    10681 gtcaccgaaa tgtttgcagt gatcgaccgg attgcggacg cgctcgagcc cggcagcgac
    10741 ggcacggtac gcggtgcgac gctggcggct ctgctggccg gtgtggatat ggagtacctg
    10801 cacgccgaag tgccgcgtgc gatcgagcag tccttggagg ggatagcgcg cattcaaacg
    10861 attgtcggtg ccatgaagga tttctcgcat ccggcgctcg accgcacgcc cattgacatc
    10921 aaccgcgcga tcggcagcac ggtcacggtc gcgaccaacg agtggaaata cgtcgcagag
    10981 gtgaagacgg aattcgacct cgatctgccc tcggttccgg tcatgcccgg aatgttcaat
    11041 caagtgatcc tcaacttgat cgtgaacgct gcgcacgcga ttgccgctgt cgcgaagtcg
    11101 aggccgcaca aaggaacgat taccgtgtcg actcgacagg tggagcgctg ggcggaggtt
    11161 cgcgtcagcg acacgggatg cgggattcct gaagcgatcc gcgaccggat attcgacccg
    11221 ttcttcacga ccaaacccgt cggcaaaggc accggccagg ggcttgcgat cgcgcacgac
    11281 gcgatcatca acaaacatcg cggcgccatc tcggtggaaa gtgaggtggg cgtgggaacg
    11341 acatttatcg tgcgtctgcc gctcgatctg gacaacgacg agatcacgaa cgccgcctga
    11401 gtacggaaaa tggtgcactg cggggctcat tttgcgggtt ccggactatt cagaactcct
    11461 gcaaactgcc gatgctcaac ggattggggg agcagtacag ccataaggat cagcggcatg
    11521 aattcggtca atttggctcc cggccgcggc aaggttcgca tcgcctttgt cgatgatgag
    11581 agcagcattc tgcagggcat gcgccgtacg ctgcacggca tgcgcaatga atgggagatg
    11641 gagttcttcg gcagcggcga ggcagcgctc gcgagccttg caagcagcgc ggcggatgtg
    11701 atcgtttcgg atatgcgcat gccgggaatg gatggctggg agtttttggc ggaggtcaag
    11761 aagcgtcatc cccagtcggt gcgactgctc ttgtccggct acgctgatcc gagttccatc
    11821 atgcgttcag tgggaaccgc gcaccaatac atggccaaac cttgcgacag tgccacgatc
    11881 aaggccgcga tcgtccagac gcagatgcta cgaagtttgc tgagcagccc gcgcctcgcc
    11941 ctgttggtgg gccacgttga attgctgccg agcacgccca aagcattcca ggaaatactc
    12001 gcctgtattc gccgacctga cgcgacgctc gtggacgccg cgcaaatcat cgcgcgagat
    12061 gtggcgatga ccgccaatat tctaaagttg gtgaactccg ccttcttcgg ttcgcgccaa
    12121 tcgatcacgc gcatcgaccg ggcggttgct tatcttggga tggatacgct tggttcgttg
    12181 gtgctcgctc acggcgtctt tcaaagcggc gtcagcatcg gcatcgaggg tttcagcttg
    12241 gaagagttgt ggcgccacag cttgcaaacg gcattgctga gtaaggcgat cgcgctcgat
    12301 cgagggctct ccgccatcaa cgtcgaggag gcgtttctcg tcggcttgct gcatgacgtg
    12361 gggaaaattg tatttgcaac tcgcgctccg gccgtcggcg attcggcaat ttcgaatgag
    12421 gaggccgtcg cgcagatgca atcccatcat gccgaggttg gcgcttacct gctcggtctg
    12481 tgggggtttc cgaattcgat cgtcgaggcg gtcgccttcc atcacaaccc aagtcaaaca
    12541 gccgatagca gcctgggtct cgccggcatc attcatatcg ccgaccgatt gtcgcgcgcg
    12601 gatggcgcga tcggcgccac gccggccgat atcggcatcg aggccgggta tttggaacgg
    12661 cacggtgtgt tgaataaatt tgcggactgg aaggcggccg cggcgggtgg cctggaacca
    12721 cagcgagctg catcatgagc gaccgcatga gcgatgcgac gagtaccgcc acgattcttc
    12781 tggtcgatga cgaaatcaac gtcaccgaag ccctgaagcg ttcctttcga cgggagccct
    12841 acacattcgt aacggccacg tcgggcgagg ccgcgctgca agtactcgcc agccggcacg
    12901 tcgacgtggt ggtctcggac gagcaaatgc ccggcatgtc aggctctgaa tttctcgcca
    12961 cggtccgggc gttatatccg cacacgatcc gcatgattct ctcgggacag gcgagcctcg
    13021 aggccgcagt gcgcgccatc aacgagggcg aggtctatcg attttttctc aaaccctgca
    13081 acccaaccga tttgttgttt acgatccaac gggcattggc gcacaagcaa ttggaagaac
    13141 aaagccgcag gctcctgcgc gagtttcaga agcaggccgc gattctcgat aagctcaaac
    13201 acacgggaga ggacttgctg cggctcgaca cggatgatca gggc
//
LOCUS       DASWZT010000284         9160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_89213, whole genome shotgun sequence.
ACCESSION   DASWZT010000284 DASWZT010000000
VERSION     DASWZT010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9160
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89213"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VGE92_07725"
     CDS             complement(<1..486)
                     /locus_tag="VGE92_07725"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1313749.1"
                     /translation="MSRFPTALLSLAAAAFAIGTSEFVIMGLLPDVASDLKVSIPQAG
                     LLVTGYAMGVVIGAPILAVLTAKLAPRPTLIFLALLFAFGNLLCALAPDFRALMIARV
                     LTALSHGTFFGVGSVVAANLVAPARRSQAIALMFTGLTLANVLGVPAGRLIGHEFGWR
                     MS"
     gene            complement(492..1310)
                     /locus_tag="VGE92_07730"
     CDS             complement(492..1310)
                     /locus_tag="VGE92_07730"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEY1313750.1"
                     /translation="MSNLMSKHFLMASDFDQTLSFNDSGLVLAEILGVKNFEQKVAGL
                     AQSNLVQQGGELAYLIRHDPEFRGVRREHLEETGRRVRLKGAIKPLVDFLEQGLSGYH
                     FSFFVISAAPKEVVVAALDGIVPQDHIFGTELNYDPHSGEVRSVMRVPAGYGKVAVLE
                     ELERRLGIAPDRTIYVGDGSSDVHVMLHVNNRDGFTIAVSENLQLARIAKSTVLSDHA
                     FSVLVPVLDQLLGWRIGQIRALFEAHGLTLHEWERARTDRVLIGETVLPAPAVA"
     gene            1460..2575
                     /locus_tag="VGE92_07735"
     CDS             1460..2575
                     /locus_tag="VGE92_07735"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY1313751.1"
                     /translation="MNKSHDYKYLQVKTALLREIESGKWAPGMRMPSEATLVRQFGVS
                     RITVGRAVKDLQLAGLIERRAGSGSFVRQPPRPQALSFGLIIPELGETDIFEPLCQGI
                     MASPLAHGHALLWGNAGGSPGVSKAGAAWQACRQYIERGVSGVFFAPLSTEPSSNELN
                     QQIVAALDEADIPVVLLDRSILPYPRPSAYDLVGIDNRSAGFVVTDHLLRHGCRRIVF
                     IGRKDGASTTDAREAGYRDALYAANAAFERSSVHRGDATDETAIRDFLSASRPDGIVC
                     ANDRTAGGVMHALRRFGRRVPGDVRIVGIDDVEYARLLPVPLTTLRQPTRQIGDVALH
                     VMLQRIARPDLPACETRLNCELIVRESCGARCAAASD"
     gene            2656..2871
                     /locus_tag="VGE92_07740"
     CDS             2656..2871
                     /locus_tag="VGE92_07740"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEY1313752.1"
                     /translation="MSENVYKVIELVGTSSESWEKATAAAINRAGKTLRDLRVAEVVK
                     LDVQLKNGKVEAYRSRISVSFKYESSK"
     gene            complement(2951..3310)
                     /locus_tag="VGE92_07745"
     CDS             complement(2951..3310)
                     /locus_tag="VGE92_07745"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEY1313753.1"
                     /translation="MTMPTFDFTALTDTYRETLAPVFAAQQTGLRTLERVARYQFAVY
                     NDYLDWALSYAKASVQPKPVADLVTEQTELNTAFGDKLRARAEELSQIATETRGNVTQ
                     WFGDTSAKVAESMKKAA"
     gene            complement(3512..4705)
                     /locus_tag="VGE92_07750"
     CDS             complement(3512..4705)
                     /locus_tag="VGE92_07750"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1313754.1"
                     /translation="MPSSVISTNWPLLLGMGVLMLGAGLQSTLLGLRATLEGFPTLVT
                     GIVLSCYYVGYVMGTKIAPPLLRKLGHIRVFAALAAIAAAAVLVQGCFVNPWSWGAMR
                     LVQGLCFAGLYVVAESWLNDRASRENRGRLFAVYMLVLYVGLGAAQFLLVLADPRTPI
                     PFMLVAVLISLAMVPIVVSAQRVPEHAVPRKVRLRDLYRNSPLGIVSVTISGLISAII
                     FSMGPVYARLSGLDTSSVATFMAVSILAAVVTQYPVGRLSDRVDRRTVIAAVCTLAML
                     ASGSIVAFPQMPPALFLVLAALFSGFALTLYSLSVSHVNDKLEPAQMVAASSALLLLN
                     GGAAAFGPVLAGSLMSAFGPPAYFATLASLSGTLAVYDLWRKSRQRPVPPAQKGPFVR
                     AQPRA"
     gene            4819..5217
                     /locus_tag="VGE92_07755"
     CDS             4819..5217
                     /locus_tag="VGE92_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313755.1"
                     /translation="MCGRTHLIIALAAALGAAPALAVEALINPTGLPTYPNLNHARMD
                     SVARTDKLGHWCNRFAADTSDALEAVEAWYRKALVNASETDLNNDERYKNYFRLTGVK
                     LAVGIDYVTVFRTADQSTTSIELFRCSPID"
     gene            complement(5227..6852)
                     /locus_tag="VGE92_07760"
     CDS             complement(5227..6852)
                     /locus_tag="VGE92_07760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEY1313756.1"
                     /translation="MFHQLLTPVGGSLALSFLVAILPIATVLVLLGILKRPAWQAAFA
                     GLAVGLAIAVTVWGMPAHIAAASAFNGAVFALWPVMWIVVNALLLYNVAVASGRFDAF
                     RAWLIRHLPNDRRVVLIAIGFCFGALLEGIAGFGAPVAITASLLIMCGFPALDALVFV
                     LIFNTTPVAFGALGTPVTVLGAVTGLPAPALAAMIGRQLPLIAGFLPFYVMALYGGWR
                     SIRALWPMLLVAGSSFGVAQFVASNYLDYTLTDVFAALGSLVVTLLFLQVWRPAADAE
                     FTIAHAAQESPVALTRPVPAWQGWLPWLIVTAIVIIWTYMKIATVGQQAIHWPGLDKA
                     VSITLYHDKPYAAVWSFQPLGTGTAILLAAIITALLVGLSPAEFCRCAVRTIRQIWQA
                     VVTVMLIVALAYVMNYSGLAYTLGLAVASTGHAFILFSPFLGWIAVFLSGSDTSGNAL
                     FGNLQVVAARQLQLNPLLFAATNSSGGVLGKMISPQNIATGVSVTDLKGKEGAVLART
                     FVHSVVLTLLLVVIVIIQQYLVPGIIPSFVASQ"
     gene            6963..8456
                     /locus_tag="VGE92_07765"
     CDS             6963..8456
                     /locus_tag="VGE92_07765"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY1313757.1"
                     /translation="MNTSMRLDGREREQRQAQIVGALAPILPSGSILFRHEDTVTYEC
                     DALTAYRTSPLVVVLPETDAQVAAILKVCHALGVPVIARGAGTGLSGGATPHHWGVIL
                     SLARFTKILDIDAATCTAVVQCGVRNAAISEAAACYGLYYAPDPSSQIACTIGGNVAE
                     NSGGVHCLKYGLTLHNVLRVRGYLADGEPVEFGSQALDAAGLDLIAVVVGSEGMLAVL
                     TEVTVKLIPKPLLARCIMASFADIESAGAAVASVIAQGIIPAGLELMDKAMTAAVEAF
                     VHADYDLSAAAILLCESDGTPEEVAEEISRMEQVLTAAGASRIEVSRDEAQRLKFWSG
                     RKNAFPASGGISPDYMCMDSTIPRKRLAEMLRAIEDMQGKYGLRCVNVFHAGDGNLHP
                     LILFDADDPDQMRRCELFGADILETSVHMGGTVTGEHGVGVEKLNSMCVQFAPAEREL
                     FLALKRAFDPGELLNPGKVIPTLQRCAEYGKMHVHRGLLPHPELPRF"
     gene            8456..>9160
                     /gene="glcE"
                     /locus_tag="VGE92_07770"
     CDS             8456..>9160
                     /gene="glcE"
                     /locus_tag="VGE92_07770"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: protein motif:HMM:NF008439.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HEY1313758.1"
                     /translation="MQQPDPALTRLVDRVLEARNRGAPLEIRGGGTKAFYGGVPRGEP
                     FEVAALCGISAYEPTELVVTARAGTRLADLEAVLAQSGQCLPFEPPRFSPGGTVGGMV
                     AAGLSGPSRAAYGCVRDHVLGVTLLSGRGEVLTFGGQVAKNVAGYDVSRLMAGSCGIL
                     GVLCDVSLKVLPIPRATATLRFDHDETRALEQLGRWSSQPLPINASAWHDGRLHVRLA
                     GAAAAVDAARVKLNGAE"
BASE COUNT         1921 a         2751 c         2872 g         1616 t
ORIGIN      
        1 gctcatacgc cagccaaact catgtccgat cagccgaccc gcgggcacgc cgagtacatt
       61 ggccaacgtc aagccggtga acatcagcgc aattgcctgc gagcgccgcg ccggggccac
      121 gagattcgct gcaaccaccg agcccacccc aaagaaggtg ccgtggctca aggccgtgag
      181 cacgcgcgcg atcatcagcg cgcgaaagtc gggcgccaac gcacacagca gattcccgaa
      241 ggcaaacagc agggccagaa aaatgagcgt cgggcgcggc gctaatttag ccgtcagcac
      301 ggctagaatc ggtgcaccga tcacgacgcc catggcatat ccggttacca acaaaccggc
      361 ctgcggaatg ctgaccttaa gatccgaggc gacatcgggc aacaggccca tgatgacaaa
      421 ttcgctcgtg ccgatcgcaa acgccgcggc ggcgagcgac agcagcgcag tgggaaatcg
      481 cgacacgcgc ttcaggcgac cgcaggggcg ggcagcacag tttcgccgat cagcacacgg
      541 tcggttcgcg cccgctccca ttcgtgcaac gtcaagccat gcgcttcgaa cagcgcgcga
      601 atctgcccga tgcgccaacc caacagttga tcgagcacgg gcaccagaac gctgaacgcg
      661 tgatcgctca gcaccgtgct cttggcgatg cgtgccagtt gtagattttc agaaacggcg
      721 atggtgaagc catcccgatt attgacatgc aacatgacgt gcacgtcgga actgccgtcg
      781 ccgacgtaaa tcgtgcgatc cggcgcgata cccagacggc gctcgagttc ttcgagaact
      841 gcgaccttgc cgtaacctgc gggcacgcgc atcaccgatc gcacttcgcc ggagtgcgga
      901 tcgtaattga gctcggtccc gaatatatgg tcctgcggaa cgattccgtc caaggcggcc
      961 accacgactt ctttgggggc tgccgatatg acgaagaacg aaaagtggta gccgctcaac
     1021 ccctgctcca ggaagtcgac caagggcttg atcgctccct tcaatcggac ccggcgaccg
     1081 gtctcttcca agtgctcgcg gcgtacaccg cgaaactctg ggtcgtgacg gatcagatag
     1141 gcgagttcgc cgccctgctg cactagattg ctttgcgcga gaccggcgac tttctgctca
     1201 aaattcttca caccgaggat ttcggccaag accagcccgg agtcattgaa actcagcgtc
     1261 tgatcgaagt ccgaggccat tagaaagtgc ttagacatca agttgctcac ggctatactc
     1321 ctcacaaagt ggatataaca actttgccct gataccctag catggagtcg cgaaagggcc
     1381 tacacattcg acgtgaaagt atttaaatgc cgagttaatt taagtatctc gacagcataa
     1441 agctgctata tcaaatgtca tgaacaagtc acacgactat aagtatttgc aggtcaagac
     1501 cgcgttgctg cgggaaatcg aatcgggaaa atgggcgccc ggcatgagga tgccgagtga
     1561 ggcgacgctc gtccgacagt tcggcgtgtc gcgaattacg gtgggccggg cggtgaaaga
     1621 cctacagctg gcggggctga tcgagcggcg cgcgggctcg ggatccttcg tgcgccagcc
     1681 gccgcgaccg caagcgctgt cctttggcct catcatcccg gagctcggcg agactgatat
     1741 tttcgagccg ctatgtcagg gaatcatggc ctcgccgctc gcacacggcc atgcgctgtt
     1801 gtggggaaac gctggcggct cgccgggcgt ctccaaggct ggcgccgcgt ggcaggcgtg
     1861 tcgccaatat attgagcgcg gtgtctccgg cgttttcttt gcgccgctct cgaccgagcc
     1921 ctcgtccaac gaattgaacc agcaaatcgt ggctgcattg gatgaggcgg acattcccgt
     1981 ggtgctgctc gatcgatcta tcctgcccta cccacgcccg agtgcctacg atctcgtcgg
     2041 catcgacaac cgcagcgcgg gcttcgtcgt cactgatcat ctattgcgcc atggctgtcg
     2101 gcgcatcgta tttatcgggc gcaaggatgg tgcatccacc accgacgccc gcgaggcggg
     2161 ctatcgcgat gccctgtatg ccgcgaacgc cgcgttcgag cgcagctcgg tgcatcgcgg
     2221 tgacgcgacc gatgagacgg cgatccgcga ttttctcagc gcgagtcgtc cggatggcat
     2281 tgtgtgtgcg aacgaccgta cggcgggggg cgtcatgcat gcactgcgcc gctttggtcg
     2341 ccgggtgccc ggcgatgtgc gcatcgtggg catcgatgat gtcgaatacg cacggctgct
     2401 gccagtgccc ttgaccacgc tcaggcagcc tacccggcaa atcggcgatg tggcactgca
     2461 tgtcatgttg caaagaattg cacgtcctga tcttcccgct tgtgaaaccc ggctcaactg
     2521 cgaactcatc gtgcgcgaat cctgtggggc acgctgcgcc gcggccagtg actgatggga
     2581 gcgatatcga ggagcgcgaa tttggaatag tatggtcagg ccgcgcgtta ccgttaatct
     2641 tgtggggaga tggtcatgtc agaaaatgtc tataaggtaa ttgagctcgt cggaaccagc
     2701 agcgaatctt gggagaaagc gaccgccgcc gcgatcaatc gcgccggcaa gaccctgcgg
     2761 gaccttcgag tcgccgaagt tgtgaagctc gacgtgcaac taaaaaacgg gaaagtggag
     2821 gcctatcgat cccgtattag tgtgtcattc aaatacgaga gcagtaagta atcacggata
     2881 ttcggaatgg ccaaaaaaaa ggcgcgacgt taaaggtcgc gcccttttgc cgttgtcgcg
     2941 ctagttcgcg ttacgcggcc ttcttcatcg actcggccac tttcgcgctg gtgtcgccga
     3001 accactgagt cacgttaccg cgggtttcgg tggcaatctg cgagagctct tctgcgcggg
     3061 cgcgcaactt gtcaccaaac gcggtattca actcggtctg ctcggtgacg aggtcggcca
     3121 ccggcttcgg ctgcacgctg gctttggcgt acgacagcgc ccaatccaga tagtcgttgt
     3181 acacagcgaa ttggtaacgc gcgacacgct cgagagttcg caatcccgtt tgctgggccg
     3241 caaagaccgg ggcgagggtt tcgcgatagg tatcggtgag ggcggtgaaa tcaaaggtcg
     3301 gcatggtcat ggcagtctcc taggtaacta acgatgttcg ggtgacgcca ttatacacag
     3361 aaaagtatgc ggtgcaaaag aaatttcttg gcgctcgcta ggctcgccat gaatcgttct
     3421 atctaattga tataattgaa ttaatgtttt tatggagatc aattcgatcg catgatggac
     3481 acgcaggaat aatgcaacgc attaaatccg gctacgcccg cggctgtgcg cgtacgaagg
     3541 gacctttttg cgccgggggc acgggccgct gccgcgactt gcgccacagg tcgtagaccg
     3601 ccagcgtgcc ggagagcgag gcgagggttg cgaagtatgc cgggggccca aatgcggaca
     3661 tcaagctgcc ggcaagcacg ggtccaaagg cagccgcccc accattcaac aacagcagcg
     3721 cgctgctcgc agcaaccatc tgcgcgggtt cgagtttgtc gttcacgtga gagacggata
     3781 aggaatagag cgtcaatgcg aaaccgctga acagtgcggc aagaaccagg aacagggcgg
     3841 gcggcatctg cgggaacgca acgatgctgc ctgaggccaa catcgcgagc gtacacactg
     3901 cggcaatcac cgtacgccga tccacgcgat cggagaggcg gccgactgga tactgggtga
     3961 ccacggcagc gagaatgctc accgccatga aagtggccac gctgctcgtg tccaagccgc
     4021 tcagccgcgc gtacaccgga cccatcgaaa atataatggc ggagatcagc cccgatatcg
     4081 tcaccgacac tattccgagc ggagaattgc gatacaaatc gcgtagccgc actttacgcg
     4141 gtaccgcgtg ctccggcacc cgctgcgccg acacgacgat cggcaccatc gccagggaga
     4201 tgagcactgc caccagcatg aagggtatcg gagtgcgcgg gtccgcaagc accaggagga
     4261 attgagccgc gccgaggccg acatagagaa cgagcatgta caccgcgaac aagcggccgc
     4321 ggttttcgcg gcttgcgcgg tcgttgagcc aactctcggc aacgacgtac aacccggcga
     4381 aacacagccc ttgtaccagg cgcatggcgc cccagctcca cggattgacg aaacatcctt
     4441 gcaccagaac cgccgcggcg gcgatcgccg cgagtgcggc gaacacgcgg atgtgtccaa
     4501 gcttgcgcag cagcggcggc gcaatcttgg ttcccatgac atagcccacg tagtagcacg
     4561 acaggacgat gccggtgacg agggtcggaa agccttcgag tgtggctcgc agcccgagca
     4621 gcgtgctttg caagccggca ccgagcatca gcacgcccat cccgagcagc agcggccagt
     4681 tggtgctaat gacgcttgat ggcacggcgg tttctattcg gggcgtgtct agacgtgtat
     4741 tcataagcta ggacagatgc ggtcccgccg cagagtatat attgacgctt tcggttcgcg
     4801 ttcgaaggag tatgaaattt gtgcggacgg actcacttga tcatcgcgct ggcggccgcg
     4861 ctcggcgcgg caccggctct cgccgtcgaa gcgctgatca atccgaccgg attaccgacg
     4921 tacccgaacc tcaatcacgc gagaatggac agcgttgcta gaaccgacaa gctcggtcat
     4981 tggtgcaatc gatttgcggc cgacacgtcc gatgcgctcg aagcggtcga ggcctggtac
     5041 cgcaaggcct tggtgaatgc gagcgaaacc gatttgaaca acgatgagcg gtacaagaat
     5101 tacttcaggc taaccggcgt caaactcgcc gtcgggatcg attacgtcac cgtgttcagg
     5161 accgcagatc aatcgacgac ctcgatcgag ctgttccgct gcagccccat cgattaggcg
     5221 ggcgagttac tgactcgcga cgaagctcgg aatgatgccg ggcacgaggt attgctggat
     5281 gatgacgatg acgaccagca ataaggtcag caccacgctg tggacaaacg tgcgcgccag
     5341 taccgcgcct tccttgccct tcaaatcggt gacggacacc ccggtggcga tgttctgcgg
     5401 cgagatcatc ttgccaagga ctcctccgga tgaattggtg gcggcaaata gcaaaggatt
     5461 gagctgcagc tgacgggcgg cgacgacctg tagattgccg aacagcgcgt tgcccgaggt
     5521 gtcgctgccg gacagaaaca cggcgatcca tcctagaaag ggcgagaaca ggataaaggc
     5581 atgtcccgtg gatgcgaccg caagacccaa ggtataggcg agacccgaat aattcatgac
     5641 gtaggcgagc gcgacaatca gcatcacggt gacgaccgct tgccagatct ggcggatggt
     5701 gcgtacggcg cagcgacaga attcggccgg cgacagaccg acgagcaacg cggtgatgat
     5761 cgctgccagc aggatcgcag tgccggtgcc gagcggttgg aagctccaca cggcggcgta
     5821 cggtttgtcg tgatacagcg tgatcgaaac cgccttgtcg agccctggcc aatggatcgc
     5881 ctgctgaccg acggtggcaa tcttcatata ggtccaaatg atcacaatcg ccgtgacgat
     5941 caaccagggt agccagccct gccaggctgg cacgggccgc gtcagcgcaa ccggtgactc
     6001 ttgcgcggcg tgagcaatgg tgaattccgc atccgccgcc ggacgccaga cctgcagaaa
     6061 cagcagcgta accaccaagg aaccgagcgc cgcgaagacg tcggtgagcg tgtagtcgag
     6121 gtagtttgaa gcgacaaact gcgccacgcc gaaactcgac ccggcgacca gcaacatcgg
     6181 ccatagcgca cggatcgaac gccagcctcc gtacagcgcc atcacataga atggcaggaa
     6241 acccgcgatc agcggcagct ggcggccgat catcgcagcc aatgcgggtg ccggcaggcc
     6301 ggttaccgcg cccaacaccg tgaccggtgt gccgagcgcc ccgaacgcga ccggcgtggt
     6361 attgaaaatc agcacgaaca ccagcgcatc gagcgccggg aagccgcaca tgatgagcaa
     6421 ggacgccgtg atggcgaccg gcgcgccgaa tccggcgatg ccttcgagca gggcgccgaa
     6481 acagaaaccg atggcgatca agaccactcg cctgtcattc ggcaggtgcc ggatcaacca
     6541 ggcgcgaaac gcatcgaaac ggccggaggc aacggcgacg ttgtagagca gcagtgcatt
     6601 gacgacgatc cacatgaccg gccaaagcgc gaacaccgcg ccgttgaacg cagacgcggc
     6661 ggcgatatgc gccggcatgc cccaaaccgt caccgcgatc gccaagccga ccgcaagacc
     6721 ggcgaacgcc gcctgccatg ccggccgctt gagtatgccg agcagaacca ggacggtggc
     6781 gatcggcagg atcgcaacca ggaaggacaa agccaaactg ccgcctaccg gtgtcaacaa
     6841 ttgatgaaac actggatctc ccccggattg ctttttgcgc atcctacgcc attagcgccg
     6901 atttcgtcat cgcgtcgccg cgcgcagccg caccgtaaac ggcgtgatgc ataatgtccc
     6961 tcatgaatac ctcgatgcgg ctcgatgggc gggagcgcga acaacgtcag gcacagatcg
     7021 tgggcgcgct ggcgccgatt ctgccgagcg gatcgatttt gtttcgccac gaagatacgg
     7081 tgacttacga gtgcgatgcg ctgaccgcct atcgcacctc gcccctggtg gtggtgcttc
     7141 cggaaaccga cgcgcaagtc gccgccatcc tcaaggtttg tcacgcactg ggtgtccccg
     7201 tcattgcgcg gggtgcgggc accggcttgt ccggcggcgc aacgccgcat cactggggcg
     7261 tgattctgtc gctcgcgagg tttaccaaaa tcctcgacat cgatgcggcc acctgcacgg
     7321 cggtcgtgca atgcggtgtg cgcaatgcgg ccatcagcga agcggccgcg tgctacggtt
     7381 tgtactatgc gcccgacccg agcagccaaa tcgcctgcac gatcggcggc aacgtcgccg
     7441 aaaattccgg gggtgtgcac tgcctgaaat acggcctgac gctgcacaat gtgctgcgtg
     7501 tacgcggcta cctggccgat ggcgagcccg tcgagttcgg ctcgcaggca ctcgacgcgg
     7561 cgggtctcga cctcatcgcg gtggtcgtgg gaagcgaagg catgctggct gtcctcaccg
     7621 aggtcaccgt gaaactcatt ccaaagccgc tgctggcacg ctgcatcatg gcgagctttg
     7681 cggacatcga atcggccggg gctgcggtgg cgagcgtgat cgcgcagggc atcattcccg
     7741 ctggactcga actcatggac aaggcgatga ctgccgcggt cgaggccttc gtgcacgccg
     7801 actacgacct cagcgccgca gcaatcctgc tgtgcgagag cgacggcacg ccggaagagg
     7861 tggctgagga aatctcgcga atggagcagg tgctgacggc cgccggggca tcgcggatcg
     7921 aggtcagccg cgacgaagcg cagcgactga agttctggag cgggcgcaag aatgccttcc
     7981 ccgcatcggg cggcatcagc ccggattaca tgtgcatgga ctcaaccatt ccgcgcaagc
     8041 gcttggcgga gatgctgcgc gcgatcgagg acatgcaagg caagtacggc ctgcgctgcg
     8101 tgaacgtgtt ccatgccggc gatggcaact tgcatccgtt gattctgttt gacgccgacg
     8161 atccggatca gatgcgccgc tgtgagctgt tcggcgcgga tattttggaa acgagcgtgc
     8221 acatgggcgg caccgtgacc ggtgaacatg gcgtgggagt cgagaaactg aactccatgt
     8281 gcgtgcagtt cgcgcccgcc gagcgcgaat tgttcctggc gctgaagcgc gcattcgatc
     8341 cgggcgagtt gctgaacccg ggaaaagtca ttccgacact gcagcgctgt gccgagtacg
     8401 gcaagatgca cgtgcatcgc ggtctgctgc cccaccccga gttgccgagg ttctgatgca
     8461 gcagcccgat cccgcgctca cccgcctcgt cgatcgtgtc ctcgaggccc gcaatcgcgg
     8521 cgcgccgctc gagatccgcg gcggcggcac caaggctttc tatggcggtg tgccgcgcgg
     8581 cgaaccgttc gaggtggccg cgctgtgcgg catcagcgcg tacgagccca ccgaattggt
     8641 ggtcacggcg cgcgccggca cgcgccttgc cgatttggaa gcggtgctgg cgcaaagtgg
     8701 tcaatgtctg ccgttcgagc cgccgcgctt ttcccccggc ggtacggtgg gcggcatggt
     8761 ggccgccggt ttgtcgggac cgtcgcgcgc cgcgtacggt tgcgtgcgcg atcatgtttt
     8821 gggggtaacg ctgttgagcg ggcgcggcga ggtgttgact ttcggtggcc aggtcgccaa
     8881 gaacgtggcc ggctacgacg tgtcgcggct gatggccggc tcctgcggaa tcttaggtgt
     8941 gctctgcgat gtgtcgctca aggtattgcc gattccccgg gcaaccgcaa ccctgcgctt
     9001 cgatcacgac gagacacggg cgctcgagca actcggccgc tggtcatcgc agccgttacc
     9061 gatcaatgcg agcgcatggc acgacgggcg actgcacgta cgtctcgccg gcgcggctgc
     9121 ggcggtggat gcggcgcgcg tcaaacttaa cggcgctgag
//
LOCUS       DASWZT010000285         2231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_36029, whole genome shotgun sequence.
ACCESSION   DASWZT010000285 DASWZT010000000
VERSION     DASWZT010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36029"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2191)
                     /locus_tag="VGE92_07775"
     CDS             complement(<1..2191)
                     /locus_tag="VGE92_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1313759.1"
                     /translation="MSAAAHSQTVAAPTQDELQEVVVTGTLIRGATAPVGSTLIAVDQ
                     AQLQSTGASTIADMLRDVPQVSTLGVAESQRSGTGGATNITLANSSNLRGLSPFATLT
                     LLDGHRIPPAGTSGSTVDPDTFPSLMLQRVDIVADGASATYGSDAIAGVANLILRRDV
                     EGVEVSAREGWASEYKDHQFDALIGHNWGSGQLSLGFENSYHSNLNGQDRSFFESDQT
                     NRGGTDYSSTQCNPGNIVVGGVSYALPGLAPNTANRCDPAKYQDILPEINHNNVALDF
                     DQKITDWISIYGDATYARRDFSNQVEQVTGPVEVPTTNAFFVAPPGAVLTPCSPAPGA
                     PNCEQVNYFFGKDLGNTAQSAGHSENYQGTIGIDFKLVNGWHLGFDGTAGKDHDRDLQ
                     TQELNNGNLAGALASSNPATALNVFGGANSAAVLNSVFANRFYAPGDTGEQVFEGKLD
                     GSLFHMPGGDVRAAVGGQWQHDELLYGINSGVPGGADLMLRQNLSRHSVSGYAEALIP
                     FFGADNAMPGLQKLELDVAGRYENYSDFGNTTHPKVGLNWSPVEGVMVHASYGTSFRA
                     PLLSELVGPLQGVFVQSYSDPMSPTGTSVGYTLGGGNLKLQPETATTYSFGVDYQPIR
                     DAKFSLNYFNIDYKNQISSYLSDLTILQQSAQLGSLITRCPSPQCTALINQYVVPGPV
                     FGPILANPSVFVNGVELNLGTTRTQGVDFVGNYAIPTDHSGTFLVGLT"
BASE COUNT          413 a          674 c          710 g          434 t
ORIGIN      
        1 cggtgagccc gaccaggaat gttcccgagt ggtcggtcgg aattgcgtag ttcccgacaa
       61 aatcgacgcc ctgcgttcgc gtcgtgccga gattcagttc cacaccgttc acgaaaacgc
      121 tgggattcgc caagatggga ccaaacaccg gaccgggtac cacgtattgg ttgatcagcg
      181 ccgtgcactg cggcgagggg catcgcgtaa tgagagatcc caactgggcc gactgctgca
      241 ggatggtcag gtcgctcaaa tagctggaaa tctggttttt gtagtcgatg ttgaaataat
      301 tcaggctgaa ctttgcgtcg cggatcggtt ggtagtccac cccgaaagaa taggtcgtcg
      361 ccgtctcggg ctgcagcttg aggttgccgc ccccgagggt gtaacccacc gacgttccgg
      421 tcggcgacat tggatccgag tacgactgca cgaacacgcc ctgcagcggg ccgaccagct
      481 cggacagcag cggcgcccgg aaggaggtgc cgtagctcgc gtgcaccatg accccttcga
      541 ccggtgacca gttcaagccg accttggggt gcgtcgtatt gccgaagtcg ctgtagtttt
      601 cgtagcggcc ggccacgtcg agctcgagct tctgcagccc gggcatggcg ttgtccgcgc
      661 cgaagaaggg aattaacgcc tcggcgtagc ctgagaccga gtgacggctc agattctggc
      721 gcagcatgag atcagcgcca ccgggaacgc cggagttgat gccgtagaga agttcatcgt
      781 gctgccactg tccgcccacg gccgcgcgga cgtcgccgcc gggcatgtgg aatagcgagc
      841 catcgagctt gccctcgaac acttgctctc cggtgtcgcc cggcgcgtag aagcggttcg
      901 cgaaaacact gttgagcacc gccgccgaat tcgctccgcc gaacacattg agcgccgtcg
      961 caggattgct gctcgccaag gcacccgcca ggttgccgtt gttgagctct tgagtttgca
     1021 agtcccggtc gtggtcctta cccgcggtgc cgtcgaaacc caaatgccag ccattaacga
     1081 gcttgaaatc gatcccgatg gtgccctggt agttctcgga atggccggct gattgggctg
     1141 tgttgccgag atccttgccg aagaagtaat tcacctgttc gcaattgggc gcgcccggcg
     1201 cgggcgagca aggcgtcagc accgcgcccg gcggcgcgac gaaaaacgca ttggtcgtcg
     1261 gcacttcgac cgggcccgtg acctgctcta cttggttcga gaagtcgcgg cgcgcgtagg
     1321 tggcgtcgcc gtagatgctg atccaatcgg tgatcttctg atcgaaatcg agcgccacgt
     1381 tgttgtggtt gatctcgggc aagatatcct gatacttcgc cggatcgcag cggttggccg
     1441 tgttgggcgc aagtccggga agcgcgtagc tgacgccgcc gacgacgatg ttgcccggat
     1501 tgcactgcgt gctgctgtaa tcggtgccgc ctcgattcgt ttgatcggac tcgaagaagc
     1561 tgcggtcctg gccgttaaga ttcgaatgat aggaattctc gaatcccagg ctcaattggc
     1621 cgctgcccca attgtggccg atcaatgcgt cgaattggtg atctttgtat tccgacgccc
     1681 agccttccct ggcgctcacc tcaacgccct cgacgtcgcg gcgcagaatg agattggcga
     1741 ctccggcaat cgcatccgag ccgtacgtcg ccgaagcacc gtcggccacg atatccaccc
     1801 gttgcagcat cagcgagggg aaggtgtccg gatcgacggt ggaaccgctg gttccggccg
     1861 gcggaattcg atgaccgtcg agcagcgtca gcgtggcgaa gggactcaag ccgcgcaaat
     1921 tgctggaatt tgccagcgtg atgttcgtgg caccgcccgt gccgctgcgc tgactttcgg
     1981 cgacgcccag agtcgaaact tgcgggacat cgcgcagcat atccgcgatc gtgctggcgc
     2041 ccgtggattg cagctgcgcc tgatcgactg cgatcagcgt cgagccgacg ggggcggtcg
     2101 cgccgcgaat caaggttccc gtgaccacga cctcctgcag ctcgtcctgg gtcggagcag
     2161 ccaccgtttg cgagtgggcg gcggcgctca acagcaatcc cgcggctgca cacagagaac
     2221 cggtgcacca a
//
LOCUS       DASWZT010000286         2038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_26488, whole genome shotgun sequence.
ACCESSION   DASWZT010000286 DASWZT010000000
VERSION     DASWZT010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26488"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VGE92_07780"
     CDS             complement(<1..1005)
                     /locus_tag="VGE92_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY1313760.1"
                     /translation="MAIAAFATLAPASGQVAPPPVNIPPIVVPPPVVVPPPVVVPPPV
                     VVPPPPVNVPPAVAPPAPSNPVITNRVTPLGWYAIGSIACAAVTPMVGTLVVGRELTV
                     TEVDRMALSCFLGPVGWVLGPVLFPDPVVIKTAMQPRNSPPKGQRLARGRHIDIPGRG
                     VISFVPNEVLLEVDAGTPDAYLNAIARRLQLRLLETQSFTLTGRTLQRWHIDGRRTVA
                     DTLRGMLPFGRISAAQPNWVYRFGQSQPPAPSDAAAAQYTVSKMHLVEAHRITNGDDV
                     LVAVIDSKIDTGHPDLTGVIADEYDALGTPGAPHVHGTGMAGAIAAHSKLIGVAPKVK
                     L"
     gene            complement(1137..1763)
                     /locus_tag="VGE92_07785"
     CDS             complement(1137..1763)
                     /locus_tag="VGE92_07785"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY1313761.1"
                     /translation="MADELDLPQHVLRFWESRFRDIKPMKRGGGRRYYRPDDVNLLRG
                     IRHLLYGEGYTIRGVQRILREQGARFVQVVWQEGAPQPPHGAAEEEDFVEDAVAAENE
                     AVEEPRGLRGRLSSLIGRDLDDRGDDLEARREPPLKGAPIPRIDPADRAVYVTQEAEL
                     PEPTQPAASAPVVSAGRLARDDLRKLHAVLHELAECRKLIESAAARTE"
     gene            complement(1867..>2038)
                     /locus_tag="VGE92_07790"
     CDS             complement(1867..>2038)
                     /locus_tag="VGE92_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY1313762.1"
                     /translation="GTTVKLSSFGSFVVRKKGQRIGRNPKTGKEVPISPRRVMVFKPS
                     AILKHRINGHAE"
BASE COUNT          389 a          651 c          642 g          356 t
ORIGIN      
        1 cagcttcact tttggcgcga cgccgatgag tttggaatgc gccgcgatcg cgccggccat
       61 gccggtgccg tgcacatgcg gagcccccgg cgtgccgagc gcatcatatt cgtcagcgat
      121 cacaccggtg aggtcgggat gaccggtatc gattttggaa tcgatcaccg ccaccaagac
      181 atcgtcgcca ttggtgatgc ggtgtgcctc cacaagatgc atcttggaga cagtgtattg
      241 cgccgcggcc gcatccgatg gtgccggcgg ctgcgattgt ccaaaccgat agacccaatt
      301 cggctgcgcc gccgaaatcc ggccgaacgg cagcatcccg cgcagcgtat cagcgacagt
      361 cctgcggccg tcgatgtgcc agcgttgcaa cgtgcggccg gtgagcgtga agctctgcgt
      421 ttccaaaagc ctcaattgca gacggcgtgc gatcgcgttg aggtaggcgt cgggcgtgcc
      481 ggcatcgact tccagcagca cttcattggg cacgaaactg attacgcccc ttcccggaat
      541 atcgatgtgc cggccacgcg cgagcctctg accttttggt ggcgaatttc tcggttgcat
      601 tgccgtcttg atgacaactg gatccggaaa caggactggg cccagcaccc agcccaccgg
      661 gccgaggaaa cacgacaagg ccatgcgatc gacttcggtg acggtcaatt cgcggccgac
      721 caccagagtg ccgaccatcg gcgtcacagc ggcgcaagca atcgaaccga tggcgtacca
      781 accaagcgga gtcactctgt tcgtgataac cggattggag ggagccggag gcgcgacggc
      841 gggcggcaca ttgaccggcg gtggcggcac tacaacgggc ggcggcacga cgacaggcgg
      901 gggcacaacg acaggcggcg gcacaacgat cggtgggatg ttgacaggtg gcggcgccac
      961 ctgccctgac gcaggagcga gcgtggcaaa agcggcgatt gccactatcg taaccacagc
     1021 aacgcgtcgc atcgtcgatt cctcagtgca tccgacaaac cgtcagcggc atcctacaag
     1081 caagcttagc gtcgtgccat gtgggactag gccgataacg cgtggaaccg ggcggcttat
     1141 tccgtccttg ccgccgcgga ctcgatgagt ttgcggcact cggcaagctc atgcagcacg
     1201 gcgtgcaact tgcgcaggtc gtcgcgtgcc agcctgccgg cactgacaac cggagcgctg
     1261 gcggccggct gcgtaggttc cggcagctcc gcctcctgcg tgacgtagac ggcgcggtcg
     1321 gccggatcaa tacgcggtat cggtgcgcct ttgagcggcg gctcgcggcg cgcctcgaga
     1381 tcatcgccgc ggtcatcgag atcgcggccg atcagcgaag acagccggcc gcgcaacccg
     1441 cgcggttcct cgaccgcttc gttttccgca gcgaccgcgt cctcgacaaa atcctcttct
     1501 tcagccgcac catgcggcgg ctgcggcgcg ccttcctgcc agacgacttg tacgaatcgc
     1561 gcgccctgct cgcgcaggat tcgctgcacg ccgcggatgg tgtagccctc gccatacaac
     1621 agatggcgaa tgccgcgcag aagattgacg tcgtcgggcc gataatagcg ccgcccgccg
     1681 ccccgcttca tcggcttgat gtcgcggaaa cggctttccc agaatcgcag cacgtgctgc
     1741 ggcagatcga gttcgtccgc cacctcacta atgctacgaa atgcgtccgg cgccttgtga
     1801 tccaaagcgg cccctttcga cgatgagttg aacgcaatac tgaaacgaaa cgaacgccga
     1861 gccggtctat tcggcgtgac cgttgatgcg atgcttgagg atcgccgacg gcttgaacac
     1921 catcacacgg cgcggcgaga tcggcacttc cttgccggtc ttaggattgc ggccgatacg
     1981 ctggcctttt ttgcgcacca cgaacgaacc gaacgaggaa agcttcacgg tggtgccg
//
LOCUS       DASWZT010000287        19836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_12046, whole genome shotgun sequence.
ACCESSION   DASWZT010000287 DASWZT010000000
VERSION     DASWZT010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 19836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19836
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12046"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VGE92_07795"
     CDS             complement(<1..561)
                     /locus_tag="VGE92_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1313763.1"
                     /translation="MLGYYVRLAVRSLRRNVVLTSLMIAAIAVGIGASMTTLTVFRAM
                     SGDPIPRKATQLFVPQIDNWGPNKPGGRSADDLNSQLGYIDAMALMKAHKARRQSAMF
                     LAALSVRPADTSNKPFKSRVRAAYADFFPMFEVPFKYGRSWTAADDDSRAAVVVITRE
                     MNDRLFGGANSVGKTLHLDSELYSVVG"
     gene            complement(565..1791)
                     /locus_tag="VGE92_07800"
     CDS             complement(565..1791)
                     /locus_tag="VGE92_07800"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY1313764.1"
                     /translation="MQLRPILAAMRRNKVGAILIAVQMAITLAILCNSLFIIQERLAS
                     SKRATGVDEANIFEIDNLWVGHPTDLRGRLAEDLAALRSVPGVVDAFNTNSYPLSNGG
                     STDGLSLHPDQPQAMALSAIYFADEHGVSTLGLKLIAGRDFNAGDIADKVGYTDLSEA
                     SVVIVTRALADRLFPDGHALGQSIYVASQKMRSQIVGIVEQLQVPWTQTGGWGSAFSE
                     SSTIEPFRFINQASDYLVRAQPGRLSEVMQAAKKKLIEVNRGRVIDEVQPLTAARAAA
                     YRDDRGLAVILGVVCAALVLVTAFGIVGLTSYWVAQRRRQIGIRRALGATKQAIVQYF
                     QTENLLIAAAGAGAGIALAVALNLWMVKCFEMHRMHLVYALAGALAVLLLGQLAVLWP
                     AMRAASIPPALATRGV"
     gene            complement(1801..3123)
                     /locus_tag="VGE92_07805"
     CDS             complement(1801..3123)
                     /locus_tag="VGE92_07805"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1313765.1"
                     /translation="MFNYYVKLALRSLKRNAVLTALMIAAIGVGIGASMTTLTVFRAM
                     AADPIPRKSGELFAVQIDNWGPARKNIVTGDEEHLQQQISYPDAVGLMNAHAARRQSV
                     MYATGFALTPANPQLLPFQVHARAVYSDFFAMFEVPFLSGHPWSAADDADRVAVVVIS
                     RELNDKVFAGANGIGKTLNLDGHDYRVVGVLDRWQPIPKFYDLNTDKYGKSDEIFLPF
                     TTAIQARAATWGNNNCGGDIGEPGWEGRLHSECVWLQFWAELPGAADVERYRTFLNNY
                     ASDQQRSGRFHWPAHTKIRDVRQWLAYQHAVSDEVRILVLVSFSFLFVCLLNAMGLML
                     AKIMGRAGDIGVRRALGANRGAIFDQCLIEAGVVGFAGGILGLALTALGLVGLRSLLS
                     EDVGRVTHLGLADVSIAVLLGIVATVLAGLYPTWRAAHVQPAWQLKAQ"
     gene            complement(3135..3842)
                     /locus_tag="VGE92_07810"
     CDS             complement(3135..3842)
                     /locus_tag="VGE92_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1313766.1"
                     /translation="MLKMTHLSKAYRTEVVETYALRDFSIHVREGEFVAVTGPSGSGK
                     TTFLTIAGLLEEFNGGEYQLDGANVRGLNDNQRSRLRNEKIGFIFQAFNLIADLNVQD
                     NIDVPLRYRGFDAAERRRRIQQSLERVGLSARARHYPAELSGGQQQRVAIARALAGTP
                     RLLLADEPTGNLDTQMARGVMELLEELHRDGSTIVMVTHDPELAVRAQREVHIVDGQV
                     VDLSAQPRLVVGASAQA"
     gene            complement(3874..5172)
                     /locus_tag="VGE92_07815"
     CDS             complement(3874..5172)
                     /locus_tag="VGE92_07815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY1313767.1"
                     /translation="MRLLQKEPVMLRDTAGQDRVIERAHLWQRHWRAVLAVLAVLFGL
                     LVLLAYVLHYAGAGVSVDRSRLSIATVERGSFVRDIAADGQVVAAVSPTLYANAVGTV
                     TLKVHAGDTVSKGQVLAVIDSPDLTAKMSQEEATAVGLSIDWQRATLEAEQKLLQLRD
                     AFNQAQVDQKTAQRELDRSRKAYELGSYTELQALKAQDSLEKAQFAFTQAKMNYESQP
                     RQNRFDIDSKKALYDRQQYLVTDLKRQVDGLDVKSPVDGQVGQVQVADRASVAKDAPL
                     LTVVDLSALEVEIKVTESLARDLRPGMSADLEGSGHHWQGSVSGVSPEVVGGQVTARL
                     RFGSEKPAGLRQSQRLSVRILIDKRDNVLMVDRGSFVDQEGGGFAYLVHGNVAERRPV
                     RLGASSIAKVEILDGLAAGDQIVVSGTDAFNGAERAILSH"
     gene            complement(5175..6533)
                     /locus_tag="VGE92_07820"
     CDS             complement(5175..6533)
                     /locus_tag="VGE92_07820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEY1313768.1"
                     /translation="MGRYALALIAMTVTVGTVNANDLRAFYELALTRDASLQAAAFQR
                     DAAIEARPQALAQWLPQITADASVARERAGFQSGPAIGSEAADCALSAAAGVQHCYGT
                     VHSLGINMSQTLWSFQAFSQLKEANFQAAAAEAGFRSAQQNLLLRVAQAYFAILSAAD
                     QLATNRAERDAFGTLLNQARTRQQTGVGPRSDVEQAQAFFDATEQSVIDAQNALDDAH
                     LALTEIVGGHAAAIAPLREDIPLLSPDPVSADEWVVSARQENFDVRIAELKMEAAERD
                     VSAQRGRALPTLLLTGSSSKLRQDEVLGGNQTLDTVGVSFSWPLFQGGAVASAVRQSR
                     ALYREAQATYDSAQRDTERQTRAAFRGIVSGIQRIGAARRAVESGHGAVEASRRNVEF
                     GTGTEFDLLNAQNNYYAALRAYSQSRYDYLTSVLTLKQQAGRLTERDLAAVDALLVEH
                     GS"
     gene            6731..7771
                     /locus_tag="VGE92_07825"
     CDS             6731..7771
                     /locus_tag="VGE92_07825"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEY1313769.1"
                     /translation="MEFSAFIDRWRPSTLRATAATLAPGVASTLIVALAATYLSDHYH
                     APPVIFALLLGMALNQLSAEARYAPGINVSARTLLRISVALLGLRITFGQIGELGWST
                     AAMVMVTVPLTIGFGWIAARTIKLDGRYGVLSGGAVGICGASAAMAIAVAWPRKDSER
                     DTVVVIACITTYSTIAMVLYPALITHLHLDALQTGRFLGGSIHDVAQVVAAGYASSPQ
                     AGDAATIVKLMRVAMLLPVVLVITVAAARGADRSQPSQKRIALLPGFLVAFVALAALN
                     GFALVAKPIASILTEASKWLLVISVAALGMKTSLREMMAVGTTAIALIAAETIFLALL
                     VLCWILLLGTGR"
     gene            complement(7768..8751)
                     /locus_tag="VGE92_07830"
     CDS             complement(7768..8751)
                     /locus_tag="VGE92_07830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HEY1313770.1"
                     /translation="MFQAVLIERDERPYRAAYTTLDEGRLPAGDVTVRIDYSTLNYKD
                     ALAITGKGPVVRQFPMVPGIDLAGCVEASDNASCGVGERVLLNGWGVGENHWGGLAQL
                     ARVKHEWLIPLPPAFTARQAMAIGTAGYTAMLCIMALERQGVAPSSGEIIVTGAAGGV
                     GSLAVAVLAQLGFRVAAVTGRPAESEFLKRLGATEVLDRALFASPGKPLGKERWAGAI
                     DVVGSHTLANVCASMRYRGVVTACGLAGGLDLPATVAPFILRGVTLVGIDSVMCPRED
                     RLEAWRRLSQDLDIEKLDLLTREIGLSQAVAGAAQLLDGQIRGRVVVDARR"
     gene            8889..9605
                     /locus_tag="VGE92_07835"
     CDS             8889..9605
                     /locus_tag="VGE92_07835"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY1313771.1"
                     /translation="MKPKRYRRVLAIDVGGSHVKARVFGRRELRQFESGPHMTPRQIV
                     DQLHKLTGDWSYEAVSIGYPGMVLHGRIVAEPHNLGSGWVGFDFHKAFGRPTRILNDA
                     AMQAVGSYDGGRMLFLGLGTGLGSAMILDGVVAPMELAHLPYKKGRTYEDYVGERGLK
                     RLGIKKWRRTVDEVVTQLCTLLEADYVVIGGGNARKLKRLPRNARLGSNDFAFLGGFR
                     IWHHAPEPAAKGSRRSISLP"
     gene            complement(9644..11395)
                     /locus_tag="VGE92_07840"
     CDS             complement(9644..11395)
                     /locus_tag="VGE92_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313772.1"
                     /translation="MKTTGMRFAAAAAAFMIGVALNTSSARVADRSNTFSVSFPAVRS
                     ATPLDGRVILLISADLNREPRSHVSPDEPLESPYIFGQNVEGIAPGAVVVVDDKAFGW
                     PARRLSLVPAGDYLVQAVLNRYETYRMPDGRTLKLPPDKGEGQQWALKPGNLYSTPVR
                     LHLDPHHPVRTALLLDQEIAAIARKADSEFVRHIRIRSELLSRFWGRDVFIGAHVLLP
                     KGFDTHPQAHYPLMVFHGHYPDDISEFRTSPPDPNLKPDYSKRFHLAGYNRIQEEESY
                     KFYQKWIAADFPRFLVIEIEHANPYYDDSYAVNSANLGPYGDAINQELIPEVERRFRG
                     IGAGWARFTYGGSTGGWEALATQVFYPDMYNGAFAACPDPIDFRAYTLINLYDDANAY
                     ALKGEASSVERPAMRNYLGEVFTTQRDANYMELAQGDHSRSGGQYDIWQAVFGPVGED
                     GYPKPIFDKATGNIDKTVAQYWREHFDLSHIIARDWGTLAPKLKGKIHVYVGTADTYY
                     LTDSVYFAQERLESLQPKYQGTVAYGDRAEHCWNGDPQLANAYSRLHYDFQYLPIILE
                     RIAAAAPAAADLTSWRY"
     gene            complement(11467..11793)
                     /locus_tag="VGE92_07845"
     CDS             complement(11467..11793)
                     /locus_tag="VGE92_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313773.1"
                     /translation="MSVEASCAGREIRVGRGNFYLPREICDTCLPNVTAVALLWRDGE
                     VLIVPLTRESAGGLLLKQRNSRGDRVIHAQEFFRSHNLPEEFELRSIPAHWSQDSAAL
                     VVSGLK"
     gene            complement(11790..12302)
                     /locus_tag="VGE92_07850"
     CDS             complement(11790..12302)
                     /locus_tag="VGE92_07850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEY1313774.1"
                     /translation="MLCVIGCGNSTRTDDGVGVYVAQTLRKALQCNPRPDVRVFDAGT
                     GGMDVMFQARAARRLIIIDAAVSGSPAGAIFRVPGEALASEPEAGYSLHDFRWQHALT
                     AGRKIFAADFPSEVTVYLIEAASLAFGLELSAPVLASANRVVEEVQAIIGAFGRPPEP
                     AMDDATRVTT"
     gene            complement(12310..12624)
                     /locus_tag="VGE92_07855"
     CDS             complement(12310..12624)
                     /locus_tag="VGE92_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313775.1"
                     /translation="MLNEEENALRERIDKMLADGLKTTTEPFPEDNHQFAAILADLQH
                     LDPKDLKGKLVLSGFTNRPYGEDKMRCQECMYYLVHRKWCDLPELAVPVEPDWWCRLW
                     RI"
     gene            complement(12706..14301)
                     /locus_tag="VGE92_07860"
     CDS             complement(12706..14301)
                     /locus_tag="VGE92_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEY1313776.1"
                     /translation="MAVQTLDISPVGRVEGDLDVRVDIENGVVKNAWTSAELFRGFEI
                     ILRDKDPQAGLVVTPRACGICGASHLTCAAWALDTAWQTEVPRNAILARNIGQLTESL
                     QSQPRYFYGLYAIDMTNKKYRNSKYYEEAVKRFAPFTGKSYEIGVTVSGKPVEIYALF
                     GGQWPHSSYMVPGGVMCAPTLSDITRSWSLLEYYKKTWLEPCWLGCTLERYEQIKSYD
                     DFQKWLDESPAHANSDLGFYWRMGLDVGLDKYGGGVGKYVSWGYLPHEDKYQKPTIDG
                     RNAALIMKSGVYDGKTDTHVLMSQDRARENMTHAWYDEGGADIHPFDRTTKARQKNAT
                     DFNGSYSWATAVNHDQLGRLEAGPFARQLVAGGKHGEAWQHYDPLILDMYKKMGGPSL
                     HLRQIARMHETVKLYRQTEHCLREFRLNDPWYIKPRERDGRGWGATEAIRGALCHWIE
                     LKDGKIKNYQIIAPTTWNVGPRDSKGLRGPIEEALMGAPIADPTDPVEVGHVARSFDS
                     CLVCTVHAHDAKSGKELARFRIG"
     gene            complement(14330..15292)
                     /locus_tag="VGE92_07865"
     CDS             complement(14330..15292)
                     /locus_tag="VGE92_07865"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313777.1"
                     /translation="MANLLWLQGGACSGNTMSFLNAEEPSAVDLVTDFGINILWHPSL
                     GVELGDNLQKILRDCASGKLALDIFVFEGTVVNAPGGTGEWNRFAGRPMKQWVRELSD
                     AATFVVAVGDCATWGGIPATAPNPSDSVGLQFLKRNHGGFLGKDFKSKSGLPVINIPG
                     CPAHPDWITQIVVAVATGRGADIGLDDLQRPKTFFQSFTQTGCTRNMHFAYKVSATEF
                     GQRKGCLFYDLGCRGPMTHSPCNRILWNRQSSKTRAGMPCLGCTEPEFPFFELNPGTV
                     FKTQTVMGVPKDLPAGVDKKGYIALTGAAKNAAPAWAEKDIFVV"
     gene            complement(15507..15728)
                     /locus_tag="VGE92_07870"
     CDS             complement(15507..15728)
                     /locus_tag="VGE92_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313778.1"
                     /translation="MAKRGNEPTGFPQSRTQPPATPGGFKDLGVTPLAVQLGLTEEQT
                     NGHWCSRCQGIWFGYTLEAECPVCGNRNG"
     gene            complement(15739..17214)
                     /locus_tag="VGE92_07875"
     CDS             complement(15739..17214)
                     /locus_tag="VGE92_07875"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY1313779.1"
                     /translation="MKTEQTQENRAWTVLVVEDDAFVRAPLARYLEENYRTLAASSGQ
                     EALMLLDREPVDLLLCGQRMSDMSGVEFFRKTRISHPESIRILVTADTDHREMIRSIN
                     EAAVYQLVSTPCQSDQIVLLIKRALESRELARRHRYLSRELKFADEVLHRQNNSMTNL
                     LQNTYEFDKLVFASELMVEVCNLARQAAATDLPVLIEGETGTGKELMARAVHFFGNRK
                     NHLFLAQNCGALTDELLQSELFGHRRGSFTGAISDRLGLFPAADGGTVFLDEISEVSP
                     SFQVSLLRFLQEGEVKPVGTHESKHCNVRIIAASNRPLKDLVAKGEFRRDLYYRLRGF
                     EIDIPPLRDRVEDIPVLAEHAAKKYSQSINRKIAGISPEVMRRLQAHPFPGNVRELEN
                     EVRRMVALASEGEFLSRKHMSPEFMRLFPAKLADLRQPDFLKGKKTLKEKVESLEAHL
                     VSQCLLRHKWNHSRAARELGISRVGLANKIKRYKLDHGDIH"
     gene            17348..18370
                     /locus_tag="VGE92_07880"
     CDS             17348..18370
                     /locus_tag="VGE92_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313780.1"
                     /translation="MTESLALSQFAAALNAVESDPATPAEKAEMLMEIAMGLQMRPKT
                     AQPIEQAQKLYRRALEICPTDSPLLRARITTRLGTALQAMPGGGDASLKAARDCYETA
                     RQTLKENGKPEELAELEMNLGLVQQSLAASGGARLHDAIQCYHRALRTFTREAFPQEF
                     SILHNNLAIAYLSIPLADERGPMREALAVQSFEEVLKVINLVDHPSEYAMIQNNLGNA
                     LQYASSGHSLENNLRALDAYDEALKVRNPRDTPFEYANTLANKANVLRNLPDDPDHPG
                     LGRVGSLSEARRLYREAERIFTTLNESQNAAVVAEALLEIEQELRSRSHRAGNGKSNG
                     EANLNA"
     gene            18380..18610
                     /locus_tag="VGE92_07885"
     CDS             18380..18610
                     /locus_tag="VGE92_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313781.1"
                     /translation="MHDVMLELVSGRLTAITIIVWMMLSIVLSIAGGAVAGLMLARKD
                     LGASLAALMGAMYGPVAAVPGILAALIVLAFI"
     gene            18621..18845
                     /locus_tag="VGE92_07890"
     CDS             18621..18845
                     /locus_tag="VGE92_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313782.1"
                     /translation="MWDMFGNSLRLFLKGKLFRDPKAVFRQWLIGFAVALLLVVVFVK
                     LSMPMWSAVVLAALGVGSLQPFLFKNLKYA"
     gene            18854..19756
                     /locus_tag="VGE92_07895"
     CDS             18854..19756
                     /locus_tag="VGE92_07895"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEY1313783.1"
                     /translation="MSSPVIRSPDAAGEPRPDDLDAFVHAITAVESIVAGWDEAQVLT
                     VQALKSAIEDFHKEALQRLIRALKDDPSAGARLRAALADPVVYGVLRFHGLIRAPLAE
                     RLNLALEEVRPSLASHGGGVELVAIKPPDTVELRLIGSCHGCPASGQTLSEGVEKAIR
                     THCPEILHINQVSRAAPEAAAANPSTVHFISPFALHAKSGWLDAGIVADIPEGAVVER
                     QLKGRSLLLSKRGEQISCFDNACAHLGMPLDMGEVRDGVITCSYHGFKYLLETGECLT
                     APEVQLKVHAVRVTGQRVQVRLEE"
BASE COUNT         3690 a         6237 c         6073 g         3836 t
ORIGIN      
        1 gccgacgacg ctgtacagct cgctgtccaa gtgaagggtc ttgccgacac tgttggcgcc
       61 gccgaacaac cgatcgttca tttcgcgcgt aatcaccacc accgccgcgc gactgtcatc
      121 atccgccgcc gtccaggagc ggccgtattt gaacggcact tcgaacatcg gaaagaaatc
      181 cgcgtatgcc gcccgcacac ggctcttgaa cggcttatta ctcgtatccg ccggccgcac
      241 cgagagcgcg gccagaaaca tggcggattg acgccgggcc ttgtgcgctt tcatcaacgc
      301 catcgcgtcg atgtagccca actgcgaatt gagatcgtcc gcagatcgac cgccgggctt
      361 gttcggcccc cagttgtcga tctgcggcac gaacaactgg gtcgccttgc ggggtatcgg
      421 gtcacccgac atcgcgcgaa acacggtgag cgtcgtcatg gacgcgccga tacccaccgc
      481 gatcgcggcg atcatcaaag aagtcagaac gacgttgcgt ctgaggctgc ggacggcaag
      541 tcggacgtag tagccgagca tcgactagac accccgcgtg gcgagcgccg gcggaatgga
      601 cgctgcgcgc attgccggcc acagcaccgc gagttgaccc agcagcagca cggcgagcgc
      661 gcccgccaga gcgtagacca gatgcatgcg gtgcatttcg aaacacttca ccatccacag
      721 attgagcgcc acggcgagcg cgattccggc gccggcgccg gccgccgcga tgagcagatt
      781 ctcggtctgg aagtactgca cgattgcctg cttggtggcg ccgagtgcgc ggcgaatgcc
      841 gatttgccgg cgacgctgcg cgacccagta gctggtgagt ccgacgatgc caaaagcggt
      901 gaccagcacc agcgcggcac aaaccacgcc gagaatgacc gccaaacccc ggtcgtcgcg
      961 gtaagccgcg gcgcgcgccg cggtcagcgg ctgcacttcg tcgatgacgc gcccgcgatt
     1021 cacttcgatg agtttctttt ttgccgcctg catcacctcc gacagccggc ccggctgggc
     1081 gcgcaccagg tagtccgacg cttgattgat gaaacgaaag ggctcgatgg tcgagctctc
     1141 gctgaacgcg ctgccccaac ccccggtctg cgtccaaggc acttgcagct gctcgacgat
     1201 gccgacgatt tgcgagcgca tcttttgcga tgccacgtag atgctctgcc cgagggcgtg
     1261 gccgtctggg aatagcctgt cagccaacgc acgcgtaacg atgacgaccg acgcctccga
     1321 caagtcggtg taaccgacct tgtcggcgat gtcgcccgca ttgaaatcgc ggccggcgat
     1381 caacttgagc ccaagggtcg aaaccccgtg ctcgtccgcg aaataaatgg cgctcaacgc
     1441 catggcttga ggttggtcgg gatgcaggct gagcccatcg gtcgaacctc cgttgctcaa
     1501 cggataggaa ttggtgttga acgcatcgac cacgccggga acgctgcgta gcgctgcgag
     1561 atcctccgcc agccggcccc tgaggtcggt cgggtgaccc acccacagat tgtcgatctc
     1621 gaaaatattg gcctcatcga cccccgtcgc acgtttgctc gaggcgagac gctcttgaat
     1681 gatgaaaaga ctgttgcaca gaatggccag cgtaatcgcc atttgcacgg cgattaaaat
     1741 ggcgccgacc ttattgcggc gcattgccgc gagaatgggc cgaagttgca tgatgtcccc
     1801 ctactgagcc ttcaactgcc aggccggttg cacgtgggcg gcgcgccacg tgggatagag
     1861 tccggccagc accgtcgcca cgatgccgag cagcaccgca atgctgacat cggccagtcc
     1921 caagtgggtt acgcggccga catcctccga caacagcgat ctcaatccga cgagcccaag
     1981 cgccgtgagc gcgagaccga gtatcccgcc tgcgaagccg accacgcccg cttcgatcaa
     2041 acactgatcg aaaatcgcgc cgcggttcgc ccctagcgcg cggcgcacgc cgatgtcgcc
     2101 ggcccggccc atgatcttcg cgagcatcag gcccatcgcg ttgagcaggc acacgaacag
     2161 aaaactgaac gacaccagca ccagtattct gacttcgtcc gacaccgcgt gctgatacgc
     2221 gagccactgc ctgacgtcgc gaatcttggt gtgcgcaggc caatggaaac ggccggatcg
     2281 ttgttggtcg gaagcgtaat tgttcaagaa cgtgcggtag cgttccacgt ccgcggcgcc
     2341 cggcaactcg gcccaaaact gcagccaaac gcactcggag tgcaaccgcc cctcccaccc
     2401 cggctcgccg atgtcgccgc cgcaattatt gttgccccag gtggcggccc gcgcttgaat
     2461 ggcggtggtg aacggcagga agatttcatc gctcttgcca tacttgtcgg tgttgaggtc
     2521 atagaacttc ggaatcggct gccagcggtc caacacaccg acgacgcgat agtcgtgtcc
     2581 atcgaggttg agcgtcttgc cgatgccgtt cgcgccggcg aagaccttgt cgttgagttc
     2641 gcggctgatg accacgacgg ccacccgatc ggcgtcgtca gcggcactcc agggatgccc
     2701 gctgagaaac ggtacttcga acatcgcaaa aaaatcgctg tagacggcgc gcgcgtgtac
     2761 ctggaacggc aagagctgcg ggttggccgg cgtcaaagca aagccggtgg catacatcac
     2821 cgactggcgc cgcgcggcat gtgcgttcat gaggccgacg gcatccggat agctgatttg
     2881 ctgctgcaga tgttcctcat cgccggtgac gatgttcttt cttgccgggc cccagttgtc
     2941 gatctgcacg gcaaacagct cgcccgattt gcgcggtatc ggatcggccg ccatcgcgcg
     3001 aaacacggtg agcgtcgtca tcgatgcgcc gatcccgacg ccgatggccg cgatcatgag
     3061 tgccgtcaga actgcgttgc gcttcaggct gcgcagggcc aacttgacgt agtagttgaa
     3121 catggacggt gccgctaggc ttgagcgctg gcgccgacaa cgaggcgcgg ctgcgcactc
     3181 agatcgacca cctggccgtc cacgatgtgc acctcgcgct gcgcccgcac ggcaagctcg
     3241 gggtcgtgag tcaccatgac gatggtcgaa ccgtcgcgat gcagttcctc gagcagttcc
     3301 atgacgccgc gcgccatctg cgtgtcgagg ttgccggtgg gctcatccgc caacagcagc
     3361 cgcggtgttc ccgccagggc gcgggcgatt gccacgcgtt gctgttgtcc gccggacaat
     3421 tcggccgggt agtgacgcgc gcgcgccgag agtccgacgc gttccagcga ttgctggatg
     3481 cggcggcgcc gctccgcagc gtcgaagcca cgatagcgca gcggcacgtc gatgttgtcc
     3541 tggacgttga gatctgcaat caggttgaag gcctggaaaa tgaagccgat cttctcattg
     3601 cgcaggcgcg agcgttgatt gtcgttgaga ccgcgcacat tggcgccgtc gagttgatat
     3661 tcgccgccgt tgaattcctc gagcagtccc gcaatggtca ggaacgtcgt cttgccggaa
     3721 cccgaaggcc cggtcacggc gacgaattcg ccttcgcgca catgaatcga gaagtcgcgc
     3781 agcgcatagg tctcgacgac ttcggttcga tacgccttcg acaaatgggt cattttcaac
     3841 atgggatggc ctcgtacggc gaattgattg gtgtcagtgg ctcaagatgg cgcgttcggc
     3901 gccattgaag gcgtcggtgc cggatacgac gatttgatcg ccggccgcga gcccatcgag
     3961 aatctcgacc ttggcaatgc ttgaagcgcc gaggcgcaca gggcgacgct cggcgacgtt
     4021 gccatgcacc aggtaggcga agccgccgcc ttcctgatcg acaaacgagc cgcgatcgac
     4081 catcaacacg ttgtcgcgct tgtcgatgag tattcgcacc gacaggcgct ggctctgacg
     4141 cagtcccgcg ggcttctcgc tgccgaagcg caggcgcgcc gtgacctgac cgccgacgac
     4201 ttcgggggat acaccgctga cgctgccctg ccagtgatgg ccgctgcctt cgaggtcggc
     4261 gctcatgccc gggcgcagat cgcgtgccag gctttccgtg accttgattt ccacttccag
     4321 cgcggacaga tcgacgacgg tgagcagcgg cgcatctttg gcgacgctgg cgcggtcggc
     4381 cacttgcacc tggcccacct ggccgtccac gggagacttc acgtccaagc cgtcgacttg
     4441 gcgtttcaga tcggtcacca ggtactgctg ccgatcgtac aacgcctttt tgctgtcgat
     4501 atcgaagcga ttctgcctcg gctgcgactc gtagttcatt tttgcctgcg taaatgcgaa
     4561 ctgcgctttc tccagggaat cttgagcttt gagcgcctgc agttccgtgt aggagcccaa
     4621 ttcgtaggcc ttgcggctgc ggtcgagttc gcgctgcgcc gttttctgat cgacctgtgc
     4681 ttgattgaag gcgtcgcgca gttgcagcag cttctgttcg gcctcgagcg tcgcgcgttg
     4741 ccaatcgatg gagagtccga ccgccgtcgc ctcttcttgc gacatcttgg ccgtcaagtc
     4801 cgggctgtcg atgacggcga gcacctgacc cttggacacc gtatcgccgg cgtgcacctt
     4861 gagcgtcacg gtgccaacgg cgttggcgta caaagtcgga ctgacggccg ccaccacctg
     4921 accatcggcg gcgatgtcgc ggacgaagct gccgcgctcc accgtcgcga tggacaatcg
     4981 cgagcgatcc accgagacac cggctccggc gtagtggagc acgtaagcaa gcagcaccag
     5041 gagcccgaag agcacggcga gaacggccag cacagcgcgc caatggcgct gccaaagatg
     5101 tgcgcgttct atcacgcgat cctgacctgc ggtgtcgcgc agcatgacgg gctccttctg
     5161 caatagacgc atactcatga tccgtgttcc accaacaagg catccacggc tgcgagatcg
     5221 cgctcggtga ggcgacctgc ctgctgtttg agggtcagca cgctcgtcag gtagtcatat
     5281 cgcgactggc tgtaggcgcg caacgccgcg tagtaattgt tctgcgcgtt cagcagatcg
     5341 aactcggtgc ccgtgccgaa ttccacgtta cgccggctcg cctctactgc gccgtgaccc
     5401 gattcgacgg cgcgccgcgc ggcgccgatt cgttggatgc cgctgacgat gccgcggaaa
     5461 gccgcacggg tctggcgctc ggtgtcgcgc tgggcggaat cataggtcgc ttgcgcctcg
     5521 cgatacagcg cccgcgattg acgcacagcc gaggcgaccg cgccgccttg aaacaggggc
     5581 cagctgaacg aaacacccac cgtatccagg gtttgattgc cgccgagcac ctcgtcctgc
     5641 cgcagcttgg aactcgaacc cgtgagcaac agcgtcggca gggcgcggcc gcgctgcgct
     5701 gagacatcgc gttccgccgc ttccattttg agctcggcga ttcgcacgtc gaaattttcc
     5761 tggcgcgcgg aaaccaccca ctcatccgct gagacggggt cgggtgacag caacgggatg
     5821 tcttcgcgca gcggtgcgat cgcggcggcg tgcccgccta cgatctcggt gagcgccagg
     5881 tgtgcgtcgt ccaaggcgtt ctgcgcatcg atgacgcttt gctcggttgc atcgaagaac
     5941 gcctgcgcct gctcgacatc gctgcgcggg ccgactccag tctgttggcg cgttctcgct
     6001 tggttcagca acgtgccgaa ggcatcgcgc tcggcgcgat tggtggcgag ctgatcggcg
     6061 gcggagagga tggcgaagta ggcctgagca acccgcaaca ggagattttg ctgtgcgctg
     6121 cgaaagcccg cctccgccgc ggccgcttgg aaattcgcct ccttgagttg gctgaatgcc
     6181 tggaagctcc acaaggtctg cgacatgttg ataccgaggc tgtgcaccgt gccgtaacag
     6241 tgttgcacgc cggcggcggc gctcagcgcg cagtcggccg cttcactgcc gatcgcggga
     6301 ccgctctgga agcccgcccg ctcgcgggcc acggatgcgt cggccgttat ctgcggcagc
     6361 cactgggcga gggcctgcgg ccgagcctcg atcgccgcat cgcgctgaaa cgcggcggcc
     6421 tgcagcgaag catcgcgagt cagcgcaagc tcatagaatg cccgcaaatc gttggcgttg
     6481 acggttccca ccgtcactgt catcgcaatc aacgccaacg cgtagcggcc catgtccttc
     6541 gcttcctccg attggattcc taggttgagt agagcaagcg gcgtgccagg ctggcgcacc
     6601 tgcaagtcgt tgtttttaat cgcggcagcg aaggcggctt gcgttcgcgc tgtccgcgga
     6661 cactgtccgc ggacagcggc ggacagttcg ccggctggcg caacgcccgg cagcgggtaa
     6721 gatctcgctg atggagtttt ccgcgtttat cgatcgttgg cgcccgagca cgctgcgcgc
     6781 gaccgccgcg accctcgccc cgggagtcgc gtccacgttg atcgttgcgc tggcggcaac
     6841 ctacctgtcg gatcactacc acgcaccgcc ggtcattttt gcactgctgc tgggtatggc
     6901 cttgaatcag ttgtccgccg aagcgcggta cgcgcccggt atcaatgtct ccgcgcgcac
     6961 gctgctgcgc attagcgttg cgctgctggg gctgcgcatt accttcggac agatcggcga
     7021 gctcggttgg tcgacggcgg ccatggtgat ggtgacggtt cccttgacca tcggatttgg
     7081 ctggatcgct gcgagaacca tcaagctcga cggccgttac ggcgtgctct ccggcggtgc
     7141 cgtcggcatt tgcggcgcgt ccgcggcgat ggcaatcgcc gttgcttggc ctcgcaagga
     7201 ctccgagcgc gataccgtcg tggtgatcgc ctgcatcacc acatacagca cgattgccat
     7261 ggtgctttat cccgccctga tcacccatct tcatttggat gcgctgcaga ccggacgctt
     7321 ccttggcggc tcgatccacg atgtggcgca ggtcgtggcg gccggctacg cctcctcgcc
     7381 gcaggccggc gatgcggcca ccatcgtcaa gctcatgcgg gtcgccatgc tgctgccggt
     7441 ggtcttggtc atcaccgtcg ccgccgcacg tggtgcggat cgaagccaac ccagccagaa
     7501 gagaatcgcg ctgctgcccg gctttctcgt cgcgttcgtg gcgctggctg cgttgaacgg
     7561 cttcgccctg gtcgcaaagc cgatcgcatc gatcctcacc gaagcgtcga agtggctgct
     7621 ggtcatttcg gtggcggcgt tggggatgaa gacctcgctg cgcgaaatga tggcggtcgg
     7681 cacgacggct atcgcgctca tcgcggcgga gacgatcttc ctcgcgctgc tggtactttg
     7741 ctggatattg ctgttgggca ccggccgcta gcggcgagca tcgacgacaa cgcggccgcg
     7801 aatctgtccg tcgagcaatt gcgcggcgcc ggcaacggct tgcgagaggc cgatttcccg
     7861 agtgagcaga tcgagcttct cgatatcgag atcctgactc agacgccgcc acgcctcgag
     7921 ccgatcctcc cgcgggcaca tcacgctatc aatcccgacg agcgttacgc cgcgcaaaat
     7981 aaatggcgcc accgtcgccg gaaggtcgag gcctccggca aggccgcacg ccgtgacgac
     8041 gccgcggtag cgcatcgagg cgcagacgtt cgccaaggta tggctgccaa ccacgtcgat
     8101 cgcaccggcc cagcgctctt tgccaagagg cttacctgga cttgcgaaca gcgcccgatc
     8161 caacacctcc gtggcgccca agcgcttcag gaattcggac tccgccggac ggccggtgac
     8221 ggcggcaacg cgaaatccga gctgggcgag cacggcgacg gcgaggctgc cgactccgcc
     8281 ggccgcaccg gtcactataa tctcgccgct gctcggcgcc accccttgcc gctcgagcgc
     8341 catgatgcac agcatcgccg tatagccggc ggttccaatc gccatcgctt ggcgcgccgt
     8401 gaatgccggc ggcagcggaa tgagccactc atgcttcacg cgggcgagct gcgccaggcc
     8461 tccccagtga ttttcgccga cgccccaacc attgagcagc acgcgctcgc cgacgccgca
     8521 ggaggcgttg tcggatgctt caacgcagcc cgccaaatcg atccccggga ccatcgggaa
     8581 ctgccgcacg acgggtcctt tgcccgtgat cgccaacgca tccttgtagt tgagcgtcga
     8641 ataatcgatg cgtaccgtga catcgccggc cggcaatcgg ccttcgtcca gagtggtata
     8701 cgccgcgcga taagggcgct catcgcgctc gatcaaaact gcctgaaaca tacattccct
     8761 ctgcccgagc cccgggtctt cgagtataaa cggcagctta tcgagatcgc ctctgccggc
     8821 tccacgcggc tcgagtatac ccagccggtc acagttcttc ggataacatt gaggtaatgt
     8881 tcggcctcat gaaacctaaa cgctaccggc gagtgcttgc catcgacgtc ggcggaagcc
     8941 acgtcaaggc acgcgtcttc ggccgccgcg agctgcggca atttgaatcc ggcccgcaca
     9001 tgacgccgcg ccagattgtg gatcaactgc acaaactgac cggcgattgg agttacgagg
     9061 cagtgtccat cggatatccc ggcatggtgc tgcacggcag aatcgtcgcc gagccgcaca
     9121 atcttgggag cggttgggtg ggctttgatt ttcacaaagc gttcgggcgc ccgacgcgca
     9181 tcctgaacga cgccgcgatg caggcggtgg gtagttacga cggcggccgc atgctatttc
     9241 tcggtctggg gacaggtctc ggatccgcca tgatcctcga cggcgtcgtt gcgccgatgg
     9301 aacttgcgca tcttccctac aagaaggggc gcacctacga ggactatgtc ggtgaacgcg
     9361 gcctgaagcg gttgggcatc aagaaatggc gccgcaccgt cgacgaggtc gtcacgcagc
     9421 tttgcaccct gctcgaagcc gactacgtcg tcatcggcgg cggcaatgca cgcaagctca
     9481 agcgcttgcc gcgcaacgcg cgcctcggca gcaacgactt cgccttcttg ggtggttttc
     9541 gaatctggca tcatgccccc gaaccggccg caaaaggctc gcggcgaagc atttccttgc
     9601 cttgagcgtc ggctcgtggc catcttccaa gcgaaatagg cgatcaatag cgccaactgg
     9661 tgagatccgc tgcggccggt gccgcagcgg cgatgcgctc gagaataatg ggcagatatt
     9721 ggaaatcgta atgtaggcgc gaataggcgt tggcgagctg cgggtcgccg ttccagcaat
     9781 gctcggcgcg atcgccgtac gccacggtgc cctggtattt cggctgcaag gactccagcc
     9841 gctcctgggc gaagtacact gaatcggtca aatagtaggt gtcggcggtg ccgacgtaga
     9901 cgtggatctt gcccttgagc ttcggtgcca gcgtccccca gtcgcgcgca atgatgtgcg
     9961 acagatcgaa gtgctcgcgc cagtactgcg ccacggtttt gtcgatattg cccgtcgcct
    10021 tgtcgaagat cggctttgga taaccgtcct cgcccaccgg cccgaacact gcttgccaga
    10081 tgtcgtactg cccgcccgag cgactgtggt cgccttgcgc cagttccatg tagttggcat
    10141 cgcgctgcgt cgtgaacacc tctcccaagt agttgcgcat cgcgggacgt tctaccgagc
    10201 ttgcctcgcc cttgagcgcg taggcgttgg catcgtcgta tagattgatg agcgtgtacg
    10261 cccggaaatc gatggggtcc gggcacgcgg cgaaggcgcc gttgtacatg tcgggataaa
    10321 acacctgagt ggccagagcc tcccatccgc cggtggatcc accgtaggtg aagcgcgccc
    10381 acccggcgcc gatgccgcgg aagcgccgtt ccacctccgg gatcaactcc tgattgatgg
    10441 catcgccgta cgggccgaga ttggctgaat taaccgcata gctatcatcg tagtacggat
    10501 ttgcatgttc gatttcgatg accaggaagc gcggaaaatc cgccgctatc cacttctggt
    10561 agaacttgta gctctcctcc tcttggatac ggttatagcc ggcgagatgg aatcgtttcg
    10621 agtagtcggg tttgaggttt ggatccgggg gtgaggtgcg aaattccgaa atatcgtcgg
    10681 ggtaatgacc gtggaacacc atcagcgggt agtgcgcttg cggatgtgtg tcgaaaccct
    10741 tgggcagtaa aacgtgggcg ccgatgaaca cgtcgcgtcc ccagaaccgc gatagcagct
    10801 cgctgcggat gcggatgtgg cgcacgaact cgctgtccgc cttgcgagca atggcggcga
    10861 tctcctgatc gagcagcagc gcggttctca ccggatgatg cggatcgaga tgtaggcgaa
    10921 cgggagtcga atagagattg cccggcttga gcgcccactg ctgcccttcg cctttgtcgg
    10981 gcggcagctt aagggtgcgg ccgtccggca tacggtaggt ttcgtaacga ttcaggaccg
    11041 cctgcaccag gtagtcgccg gcgggaacga gcgagaggcg tctcgccggc cagccgaacg
    11101 ccttgtcatc cacgacgacc acggccccgg gagcgatgcc ttcgacgttc tggccgaaaa
    11161 tataggggct ttccagcggt tcgtcgggac tcacgtgaga acgcggttcg cggttcaagt
    11221 ccgcactgat cagcaggatc actctgccgt cgagcggcgt ggcgctgcgc accgcgggga
    11281 agctgaccga gaacgtattg cttcgatcag cgacgcgagc gctgctggtg ttgagcgcca
    11341 cgccgatcat gaaagcagca gcagccgctg cgaatcgcat ccccgttgtc ttcattctgc
    11401 acccgaagcg taaagtaggc tgcgaacgct ggctgaatcg ccgtgatcag tcaagttctc
    11461 gagacgctac ttcagcccgc tgacgaccaa cgccgcggaa tcctgactcc aatgcgccgg
    11521 tatcgacctc aactcgaatt cttcgggtaa attatggctg cggaaaaact cttgcgcatg
    11581 tatcacccga tcgccgcggg agttgcgctg tttgagcaac aacccgcccg cggactcccg
    11641 ggtcaagggg acgatcagga cctcgccatc gcgccacaag agcgcgaccg ccgtcacatt
    11701 cggcaagcac gtatcgcaaa tttcccgcgg caggtagaaa ttgccgcggc ccacgcggat
    11761 ctcgcgacca gcgcaactcg cctcgacgct catgttgtga cgcgcgtggc atcatccatc
    11821 gccggctccg gcggacggcc gaaagcgccg atgatcgcct ggacctcttc gaccacccga
    11881 ttggcggaag ccaacaccgg cgcggagagt tccaggccaa atgccagact cgctgcctcg
    11941 atcaggtaca ccgtgacttc gctcggaaaa tcggcggcga aaatcttgcg tcctgcagtg
    12001 agcgcgtgct gccagcggaa gtcgtgcagg ctgtagcccg cctccggttc gcttgccaat
    12061 gcctccccgg gtactctgaa aatcgcgccg gccggtgagc cgctgaccgc cgcgtcgatg
    12121 atgatcaagc ggcgcgcggc gcgcgcctga aacatcacat ccatcccgcc cgtgccggca
    12181 tcgaagacac ggacgtcggg ccgtggattg cattgcagcg ccttgcgcaa ggtttgggcc
    12241 acgtagacgc ccacgccgtc gtcggtacgg gtcgagttac cgcagccgat cacgcacagc
    12301 atgcacgtcc tagatacgcc acaggcgaca ccaccagtca ggctccacgg ggacggcaag
    12361 ctcgggaaga tcgcaccact ttcgatgcac caggtagtac atgcactcct ggcagcgcat
    12421 cttgtcctcg ccgtacggac ggttggtgaa tccggacagc accaacttgc ctttgaggtc
    12481 cttcgggtcg agatgctgca agtcagcgag aatcgccgcg aattggtggt tgtcttccgg
    12541 aaacggctcg gtcgtggtct tcaggccgtc cgccaacatc ttgtcgatcc gttccctgag
    12601 ggcgttttcc tcctcattca gcataatccc accttccagc acgccggaga acttaagacc
    12661 gcagccaacc aatcatgcca cgcggcctcg tctttattac agccgtcagc cgatccggaa
    12721 cctcgccagc tccttgccgc tcttggcgtc gtgcgcgtga accgtgcaaa ccaggcaaga
    12781 atcgaaggag cgcgccacat ggcccacttc gaccgggtcc gtcggatctg cgatcggtgc
    12841 gcccatcagc gcctcctcga tcggtccgcg aagacccttg gaatcccgcg gtccgacatt
    12901 ccaggtggtc ggcgcgataa tttggtagtt cttgatcttg ccgtctttca gctcgatcca
    12961 atgacagaga gctccccgga tcgcctcggt ggctccccag ccgcgtccgt cgcgctcgcg
    13021 cggcttgatg taccaaggat cgttcaagcg gaattcgcgc aggcaatgct ctgtctgccg
    13081 atacagtttc acggtctcgt gcatgcgcgc gatctgccgc aaatgcagac ttgggccgcc
    13141 cattttcttg tacatatcca agatcaaggg atcgtagtgt tgccatgcct ccccgtgctt
    13201 gccgccggcg accagttggc gcgcgaacgg acccgcctcc aagcggccaa gctgatcgtg
    13261 attgacggcc gtcgcccagg aataactgcc gttgaaatcg gtggcattct tttgccgcgc
    13321 tttggtggtg cggtcgaacg gatgaatgtc ggcgccgcct tcgtcgtacc acgcgtgcgt
    13381 catgttctcc cgtgcgcgat cctgactcat gagcacgtga gtatccgtct tgccgtcgta
    13441 tacgccgctc ttcatgatca atgccgcatt gcggccgtcg atcgtcggct tctggtactt
    13501 gtcctcgtgc ggcaaatagc cccaactcac atacttgccg accccgccgc cgtatttgtc
    13561 gagcccgaca tcgaggccca tgcgccagta aaagccgaga tccgaattgg catgcgccgg
    13621 actttcgtcc agccatttct ggaagtcgtc gtagctcttg atctgctcat aacgctccag
    13681 cgtgcacccc agccagcagg gttcgagcca ggttttcttg tagtattcga gcagcgacca
    13741 ggagcgcgtg atatccgaca gcgtcggcgc gcacatgacg ccgcccggca ccatgtagct
    13801 cgagtgcggc cactggcccc cgaacagggc gtagatctcg acgggcttgc ccgacacggt
    13861 aacgccgatc tcgtaggact tgccggtgaa cggcgcgaag cgcttcaccg cctcctcata
    13921 gtacttgctg ttgcgatact ttttgttggt catgtcgatc gcatataggc cgtagaagta
    13981 gcgcggctgg ctttgcaggc tctcggtcaa ttgaccgatg ttgcgtgcca ggatcgcatt
    14041 gcgcggtacc tcggtttgcc acgcggtgtc gagcgcccac gcggcgcagg tcaggtgcga
    14101 ggcgccgcaa atgccgcatg cgcgcggcgt cacgacgagt cccgcctgcg gatctttgtc
    14161 gcgcaggata atttcaaaac cgcggaataa ttccgccgag gtccaggcgt tcttgaccac
    14221 gccgttttcg atgtccacgc gaacgtccag atcgccttcg actcgtccga ccggcgagat
    14281 gtccaatgtt tgcactgcca tgattgcaac tcctgaatac tgggtgtggt caaacgacga
    14341 agatgtcttt ttcggcccag gcgggagccg cattttttgc cgcgccggtc agcgcgatgt
    14401 agcccttctt gtcgaccccg gccggcaagt ccttcggcac acccatcacc gtctgggtct
    14461 tgaacacggt gccgggattc aattcgaaga atgggaattc cggctccgta cagccaaggc
    14521 atggcatacc cgcccgtgtc ttggaagatt ggcgattcca caggatgcga ttgcagggcg
    14581 agtgcgtcat cggcccacgg caacccaagt cgtagaacag acatcccttg cgttgaccga
    14641 attcggtggc cgacaccttg taggcgaagt gcatgttgcg cgtgcaaccg gtctgggtga
    14701 agctttggaa aaacgtcttg ggacgctgta aatcgtccaa gccgatgtcc gcgccccgtc
    14761 ccgtcgccac cgcgacgact atctgcgtga tccaatccgg atgcgcgggg cagcccggaa
    14821 tgttgatcac cggcagcccg gacttggact tgaaatcctt gcccaagaaa ccgccgtgat
    14881 tgcgcttcag gaactgcaga cccaccgaat cgctgggatt cggcgcggtc gcggggatgc
    14941 cgccccacgt ggcgcaatcg cccaccgcga ccacgaaagt ggcagcgtcg ctcaattcgc
    15001 gcacccactg tttcatcggt cttcccgcga agcgattcca ttcaccggta ccgccgggag
    15061 cgttcaccac cgtgccctcg aagacaaaaa tgtcgagagc gagcttgccg gaggcgcagt
    15121 cgcgcaagat tttctgcaaa ttgtcgccga gttcgacccc gagcgaggga tgccacaaga
    15181 tattgatgcc gaaatcggtc accaaatcga ctgcactggg ttcttcggca ttcaggaacg
    15241 acatggtgtt gccggagcac gcaccaccct gcagccagag taggtttgcc atctcgaaag
    15301 tcccccgcgt tgagtttttt ggaaaaacag cccacgaggc gcgcatcgcg ggcctcgccg
    15361 tggcaaacga gttgcaatac gtgtgccaga tgcctattca ttgcattttg agaattatca
    15421 gcgacttgct cagtcgcggg gatccgcgag tgcaaagtga atttacactt ttcgaggaat
    15481 cgcaaagaag atatacagat ccgcgatcag ccgttgcggt tgccgcacac cgggcattcg
    15541 gcttccagcg tgtagccgaa ccagatcccc tgacagcggc tgcaccagtg gccgttggtc
    15601 tgctcttcgg tcaaaccgag ttgcacggcg agcggtgtga cgccgaggtc cttgaaaccg
    15661 cccggagttg ccggcggttg cgtccgcgat tgtggaaatc ccgtcggttc attgccgcgc
    15721 ttcgccatgc acgtaccctc aatgaatgtc gccgtgatcc agcttatagc gtttgatttt
    15781 gttggcaagg ccgacgcgcg aaattccgag ttcacgcgcg gctcggctgt gattccattt
    15841 atgccgcagc aggcattgcg ataccagatg cgcctcgagc gattcgacct tttccttgag
    15901 agttttcttg cccttgagaa aatcaggctg gcgcagatcg gcaagtttcg cgggaaatag
    15961 ccgcatgaat tccggcgaca tgtgcttgcg cgacaagaac tcgccttcac tcgcgagcgc
    16021 gaccatgcgg cgcacctcgt tttccagctc gcgcacgttt cccggaaacg gatgcgcttg
    16081 caagcgccgc atgacctctg gagaaatgcc ggcaattttg cggttgatgg attgcgagta
    16141 ttttttcgcg gcgtgctccg cgagcaccgg aatatcttcg acgcggtcgc gcagcggcgg
    16201 aatatcgatt tcaaagccgc gcaagcggta gtacaggtcg cgcctgaatt ccccctttgc
    16261 gaccagatct ttcagcggcc gattggaggc ggcgatgatg cgcacattgc agtgtttgct
    16321 ctcatgcgtg ccgaccggct tgacttctcc ttcctgcagg aagcgcagca agctgacctg
    16381 aaacgacggc gagacctccg agatttcatc gagaaacacc gtgccgccgt cggccgccgg
    16441 aaacagtccg agccgatcgc tgatggcgcc ggtgaacgag ccgcgccgat ggccgaacaa
    16501 ttcggattgc agcaattcat cggtcaaggc accgcagttc tgcgcgagaa acagatggtt
    16561 cttgcggttg ccgaaaaaat gcacggcgcg ggccatcaat tccttgccgg ttcccgtctc
    16621 cccttcgatc agcacgggca agtcggtcgc cgccgcttgg cgtgccaagt tgcatacctc
    16681 gaccatcaac tcgctggcaa ataccagctt gtcgaactcg taggtgtttt gcagcagatt
    16741 cgtcatggaa ttgttctgcc gatgcaaaac ctcgtcggcg aacttgagct cgcggctgag
    16801 atagcgatgg cggcgcgcca attcgcggct ctcgagcgcg cgtttgatca gtaagacgat
    16861 ttgatcggac tggcaaggcg tcgacaccag ctggtacacc gccgcttcgt tgatcgagcg
    16921 gatcatctcg cgatgatcgg tgtcggcggt gacgagtatg cggatggatt cgggatgcga
    16981 aatgcgtgtc ttgcgaaaga actccacgcc gctcatgtcg ctcatgcgct ggccgcaaag
    17041 cagcagatcg accggttcgc ggtccaacaa catcagggct tcttgacccg atgatgcggc
    17101 caaggtgcgg tagttctcct cgagatagcg ggccaagggt gctctgacaa aggcatcatc
    17161 ctcgaccacc aggacggtcc acgcccgatt ttcctgtgtc tgctctgttt tcatgccggc
    17221 cccccagcgt cagtgtaaag atagtttaca ttttgtaatg atatttgaca tcgcatttcg
    17281 tggctcccta cactccgctg aaagtcgaag aacgatgggc gagtcagtgg gcgcgaagga
    17341 gttggcggtg actgaatctc tggccttgtc tcaattcgca gctgcgctca acgccgtcga
    17401 atccgacccg gcgacccccg ccgagaaggc cgaaatgctc atggaaatcg ccatgggctt
    17461 gcagatgcgc cccaagactg cccaaccgat cgagcaggcg cagaaattgt accggcgcgc
    17521 actcgagatc tgtcccacgg actcgccact gcttcgcgcg cgaatcacga cgcgcttggg
    17581 cacggccttg caagccatgc cgggcggggg cgacgcatcg ctgaaagcgg cgcgcgactg
    17641 ctatgagacg gcacggcaga cgctcaagga gaacggtaag ccggaagaac tcgccgagct
    17701 cgagatgaat ctagggctgg tccagcaatc gctcgctgct tccggcggcg cgcggctcca
    17761 cgatgccatc cagtgctacc accgcgcgct gcgcaccttc acccgcgaag cgttcccgca
    17821 ggaattctcg atcctgcaca acaatctggc catcgcctac ttgtcgattc ccttggcgga
    17881 tgagcgcggc ccgatgcgcg aggcgttggc cgtgcagtcc ttcgaagaag tgctgaaggt
    17941 gatcaacctg gtggatcacc cgagcgaata cgcgatgata caaaataatc tcggtaatgc
    18001 gttgcagtac gcatcgagcg gccattcgct ggaaaataat ttgcgcgcgc tcgacgcgta
    18061 cgacgaggcg ctcaaagtcc gcaatccgcg cgatacgccc ttcgagtatg ccaacaccct
    18121 cgccaacaag gccaatgtac tgcgcaacct gcccgatgat cccgaccacc cgggactcgg
    18181 gcgcgttggg agcctgagtg aggcgcggcg tttgtaccgc gaggcggagc ggattttcac
    18241 gacgctgaat gaatcgcaga acgccgccgt ggtcgccgag gcgttgctgg aaatcgagca
    18301 ggaacttcgc agccgaagcc atcgagccgg caacggcaag agcaacgggg aagcgaatct
    18361 caatgcatag ggccgacgaa tgcacgacgt aatgctggag ctcgtcagcg gtcgattgac
    18421 tgccatcacc atcatcgtgt ggatgatgct gtccatcgtg ttgtcgatcg cggggggcgc
    18481 ggtcgcgggc ctgatgctgg cgcgcaaaga tctgggcgcg agcctcgcgg cgctcatggg
    18541 agcgatgtac ggtccggtgg ccgcggtgcc gggcattctt gcagcactca tcgtgctggc
    18601 atttatctag gtcttaggtt atgtgggaca tgttcggcaa ttcattgcgg ctttttctca
    18661 agggcaagct attccgcgat ccgaaggcgg tgtttcgcca gtggctgatc ggttttgcgg
    18721 tggcgctgtt gctggtcgtc gtgttcgtca aattgagcat gccgatgtgg tccgcggtgg
    18781 tgctggcggc gcttggcgtc ggatcgttgc agccctttct gttcaaaaac ctcaagtacg
    18841 cctgacaccg cccatgagca gccccgtcat acgatcccct gacgcggccg gcgaacctcg
    18901 ccccgatgac ctcgacgcgt tcgtgcatgc catcaccgcc gtggaatcca ttgtggcggg
    18961 gtgggacgag gcgcaagtct tgaccgtgca agcccttaaa tccgcgatcg aggatttcca
    19021 caaggaagca ttgcaacgcc tgattcgcgc gctcaaggac gacccgtccg ccggtgcccg
    19081 cctgcgcgcg gcgttggccg atccggtcgt ctacggcgtc ctgcgttttc atggactcat
    19141 cagagcgccg ctggccgaac ggctgaacct ggctttggaa gaagtgcggc cgtcgctggc
    19201 gagccacggc ggcggcgtcg aattggtggc gatcaagccc cccgataccg tggaattacg
    19261 gctgatcggc tcttgccatg gctgtcctgc ctccggtcaa acattgagcg aaggcgtgga
    19321 gaaggccatc cgcacacatt gtcccgagat tctgcacatc aaccaggtga gtcgggcagc
    19381 gcctgaagca gcagccgcca acccatcgac ggttcacttc atcagcccct tcgcgctgca
    19441 tgccaagtcg gggtggctgg acgccggcat cgtcgcggac atcccggaag gggcggtggt
    19501 ggaacgccaa ctcaaagggc gctccctatt gttgtccaag cgcggtgagc aaatcagctg
    19561 cttcgacaat gcctgcgcgc acctcgggat gcccctcgat atgggcgagg tgcgcgacgg
    19621 cgtgatcacc tgctcctacc atggttttaa gtacctgctc gagaccggcg agtgcctcac
    19681 cgctcccgag gtgcaactca aggtgcacgc ggtacgcgtc accggtcagc gcgtgcaagt
    19741 gcggctcgag gaatgacctg atgtccgcag cagtgcatgt caccctgaca ccggcattga
    19801 agcgcgagcc ggcgccggcg gcgctcgcgg tgctcg
//
LOCUS       DASWZT010000288         2058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_2293, whole genome shotgun sequence.
ACCESSION   DASWZT010000288 DASWZT010000000
VERSION     DASWZT010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2293"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /gene="bfr"
                     /locus_tag="VGE92_07900"
     CDS             complement(<1..448)
                     /gene="bfr"
                     /locus_tag="VGE92_07900"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012273886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEY1313784.1"
                     /translation="MQGDAKVLEFLNQALKNELTSINQYFLHARMLDNWGMKRLGKFE
                     YDESIDEMKHADRLVKRILFLEGLPNLQDLGKLFIGEDVQEILQCDLKREMAAHPLYK
                     TAIAHCESVKDYVTRELLVHIQKSEEEHIDFIEEQLGLIDKMGLQNY"
     gene            complement(536..757)
                     /locus_tag="VGE92_07905"
     CDS             complement(536..757)
                     /locus_tag="VGE92_07905"
                     /inference="COORDINATES: protein motif:HMM:NF016230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY1313785.1"
                     /translation="MIVCICNAVSERHIKSAVKNGAASLRDLTRELGVGNCCGKCVPE
                     ARACLSAALDSRSDAAAVRAYFPRSELAV"
     gene            888..>2058
                     /locus_tag="VGE92_07910"
     CDS             888..>2058
                     /locus_tag="VGE92_07910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY1313786.1"
                     /translation="MNRAIAAALACATACTALAMAGCGKSGGGAEAPPPPQVSVAEVI
                     ERRVKDWDEFTGRLQAVETVEIRPRVSGYIDRVAFTEGSQVKAGDLLFIIDPRPYRAD
                     YDRAAADVKRYKTALELARIELVRVQRLKDSGAVSEEELDERKSTVAQAEANVAGSEA
                     ALEAASLNLSFTQVTSPINGRVSRAEVTRGNLVTGGSNGGTLLSSVVSMDPIYLYFDA
                     DEQSYLRYAQMARSGERPSSRDSQNPVQVGLADEEGFPHAGSVDFIDNQLNPQTGTIR
                     ARAVLANKDGRFTPGLFARVQLLGSGEYSAILIEDRAVNTDQSQKYVLVLGPNNQIEY
                     RKVTLGRVIDGLRIVRAGLKAGDVIVVNGAQRVHPGVTVTPQRVTMGAAETGANAA"
BASE COUNT          392 a          601 c          682 g          383 t
ORIGIN      
        1 cgtagttctg caatcccatt ttatcgatga gcccgagctg ctcttcgatg aagtcgatgt
       61 gttcttcctc gcttttctgg atgtggacca gcagctcgcg agtgacgtag tccttgaccg
      121 attcgcagtg cgcgatggcg gtcttgtaca acgggtgcgc agccatttcg cgcttcaagt
      181 cgcactgcaa gatctcctgc acgtcctcgc cgatgaacag cttgccgaga tcttgcaggt
      241 tcggcagtcc ttcgaggaac aggatgcgct tgaccagccg atccgcatgc ttcatctcgt
      301 cgatcgactc gtcgtactcg aacttgccga gccgcttcat accccagttg tcgagcatgc
      361 gggcatgcag aaaatactgg ttgatggagg tgagttcgtt cttcagcgct tgattgagga
      421 actccaaaac tttagcgtca ccctgcacgg cagccccttg gattgaacag cgcccgaatt
      481 gcgcgcgtga gggcggggat tgtatgcccg atgccgggct caaggcaccg gttggtcaga
      541 cggcgagttc gctgcgcggg aagtaggcgc gcaccgcggc ggcgtcgctg cgcgagtcga
      601 gcgcggccga caagcaggcg cgggcctcgg gcacgcattt tccacagcag ttgccgacgc
      661 cgagctcgcg cgtcaggtcg cgcaggctcg ctgcgccgtt cttgaccgcg gacttgatgt
      721 ggcgctcgga gaccgcattg cagatgcaaa caatcatgcg ctattgtgaa cgcgaatgcg
      781 aatgattgtc aataacattc aatttaacga aatcgatgcg aatcggcgcg actctgtatg
      841 gtgaaataac ggcgccgaag cggccaggcg gatgagggag aatcggggtg aatagagcaa
      901 ttgcggccgc cttggcgtgt gccacggcgt gtacggcgct ggctatggcc gggtgcggga
      961 agtcgggcgg gggcgccgaa gcgccgccgc cgccgcaggt cagcgtcgcc gaggtgattg
     1021 agagacgcgt caaggattgg gatgaattca cgggccgtct gcaggcggtc gagactgtcg
     1081 aaatccggcc ccgggtctcg ggctacatcg acagggtggc cttcaccgag ggtagccagg
     1141 tcaaagcggg cgacctgttg ttcatcatcg atccgcgccc ttatcgagcc gactacgatc
     1201 gcgcggccgc cgacgtcaag cgctacaaga ccgccttgga gctggcgcga atcgagttgg
     1261 tccgcgtgca gcgtttgaag gacagcggcg cggtgtccga agaggaattg gacgaacgca
     1321 agagcacggt tgcgcaggcc gaagccaacg tcgccggatc cgaggctgcg ctcgaggcgg
     1381 cgtcgctcaa cttgagtttc acgcaggtca cgagcccgat caacgggcgc gtcagccgcg
     1441 ccgaagtgac gcgcggcaat ctggtgaccg gcggcagcaa cggcggcacc ttgctctcct
     1501 cggtggtgtc gatggatccg atctatttgt atttcgatgc cgacgaacag agctatttgc
     1561 gctatgcgca aatggcgcgc tcgggcgagc gcccgagttc gcgcgattcg caaaatccgg
     1621 tgcaggtggg gctcgccgac gaggaaggtt ttccgcatgc cggcagcgtc gacttcatcg
     1681 acaaccagct caatccgcaa accggcacga tccgcgcacg cgcggtgctc gcgaacaagg
     1741 acggccgatt cacgcccgga ctattcgccc gcgtgcaatt gctcggcagc ggcgagtact
     1801 cggcgatttt gatcgaggat cgcgcggtca ataccgacca gagtcaaaag tacgtgctgg
     1861 tcctcgggcc caataaccag atcgaatacc gcaaggtgac gctcggacgg gtgatcgacg
     1921 gcttgcgcat cgtgcgcgcg ggcctgaagg cgggcgatgt gatcgtggtg aacggtgcgc
     1981 aacgcgttca tcccggcgtc accgtcacgc cgcagcgggt cacgatgggc gccgccgaga
     2041 cgggcgccaa tgcggcca
//
LOCUS       DASWZT010000289         1837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_94345, whole genome shotgun sequence.
ACCESSION   DASWZT010000289 DASWZT010000000
VERSION     DASWZT010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94345"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="VGE92_07915"
     CDS             <1..870
                     /locus_tag="VGE92_07915"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY1313787.1"
                     /translation="RVNMAPAELDPHSRRLRHVCGLDLQAVNDTQVRAARRAYYGAVS
                     YVDDQVGMLLAALEEARFADNTVVMLLADHGDMLGERGLWYKMNFFEPACRIPLLVHA
                     PGRFAARRVAGSASLIDILPTLGELAGGKSDAYATPLDGTSLVPQLNGARGADEVIGE
                     YLAEGAISPLVMIKRGRYKFVHCPVDPDQLYDLKEDPDELRNLAADPSQSARLQALRT
                     EVAQRWDLPTLHAAVIASQRRRHLVYDALRLGRYTPWDFQPVRDASRLYVRNDQDLGD
                     LEAMARFPPFNPQ"
     gene            complement(857..1396)
                     /locus_tag="VGE92_07920"
     CDS             complement(857..1396)
                     /locus_tag="VGE92_07920"
                     /inference="COORDINATES: protein motif:HMM:NF016180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HEY1313788.1"
                     /translation="MGSAPGLISRIEASGLSVAGMQSMEVAALRYFDATGPFAAAVQD
                     SVGAALPAPLTAVSVSAPGGGRCILAWRSPTETWLLCDDSAVFAELEEELLSPAGCMV
                     NQTGGLCALQLRGARVAELLSRLGSAASLAGAGEARSSRVADLHVLIICVQQGEWILM
                     VERVYVAHLLDWIAKTIAD"
     gene            complement(1389..>1837)
                     /locus_tag="VGE92_07925"
     CDS             complement(1389..>1837)
                     /locus_tag="VGE92_07925"
                     /inference="COORDINATES: protein motif:HMM:NF020253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEY1313789.1"
                     /translation="IEALEIMRTEKGYIHIGTDTDGTTLPQDIGFARTLRKAANFVGR
                     RSLSRPAASDPMRLQLVALAPLDRRTLLPVGAQIAAGAPPTQTEGHVTSSYWSPALEM
                     PVALGMLTGGAQRQGERLRVHHLGTVIEAEVVKAPFIDPAGERLRG"
BASE COUNT          315 a          640 c          579 g          303 t
ORIGIN      
        1 cgcgtgaaca tggcgcctgc agagctcgat ccccattcgc ggcgcctgcg ccatgtctgc
       61 ggcctcgatc tgcaagctgt gaacgacacg caggtgcgtg ccgcgcgccg cgcctactac
      121 ggcgcggtgt cctacgtgga cgatcaagtg gggatgctgc tggccgcgct cgaggaggct
      181 cgattcgccg acaacaccgt cgtgatgctg ctcgccgatc acggtgacat gctgggtgag
      241 cgcggtcttt ggtacaagat gaatttcttc gaacccgcgt gccggatccc gctgctcgtt
      301 cacgcgcccg ggcgcttcgc cgcccgccgc gtcgcgggct cggcgtcgct catcgacatt
      361 ctgcccacct tgggcgaatt agccggcggc aaatccgacg cctacgcgac cccactcgat
      421 ggcacgagct tggtgccgca actcaacggc gcgcgcggcg cggatgaagt catcggcgaa
      481 tatctcgccg agggcgcgat ctcgccgctg gtgatgatca aacgcggacg ttataaattc
      541 gtgcattgcc cggtcgatcc cgaccagctc tacgacctta aggaggaccc cgacgagctg
      601 cgcaatcttg ccgccgaccc gtcacagtcg gcgcgtctcc aggcgcttcg caccgaggtg
      661 gcacagcgct gggatttgcc cacgctgcac gcggcggtca tcgccagtca gcggcgccgg
      721 catctcgtct acgatgcgct gcggctgggg cgctacactc cctgggattt ccaacccgta
      781 cgcgacgcga gccgcctgta cgtgcgcaac gatcaggact tgggcgattt ggaagccatg
      841 gcgcgatttc cgccgttcaa tccgcaatag tcttcgcgat ccaatcgagt agatgcgcga
      901 catagacgcg ctcgaccatc aggatccact caccctgttg aacgcagatg atcaacacgt
      961 gcaagtccgc gacccggctg ctgcgcgcct cgccggcacc tgcgagcgat gcggctgaac
     1021 cgaggcgcga cagcaactcc gcaactcgag cgccgcgcag ctgcaaggcg cataggccgc
     1081 cggtttgatt caccatgcag ccggcgggac tcaagagctc ctcctcgagc tccgcaaaca
     1141 ccgccgagtc gtcacacagc agccaggttt cagtcggact ccgccaagcg aggatgcatc
     1201 ggccgccgcc cggtgcgctc accgacacgg cggtcaaggg agcgggcagc gctgcaccga
     1261 cactgtcttg cacagcggcg gcgaatgggc ccgtcgcgtc gaagtagcgc agcgccgcaa
     1321 cctccatcga ttgcatgccc gcgacgctaa ggccggatgc ttcgatcctc gagatgagcc
     1381 ccggcgcgct acccacggag gcgctcgccg gccggatcga tgaacggcgc cttcaccacc
     1441 tcggcctcga tcaccgttcc caaatgatgg acgcgcaacc gctcgccctg acgctgcgcg
     1501 ccaccggtca acattcccag ggccaccggc atctcgagcg ccggactcca ataactcgag
     1561 gtcacgtgcc cttcggtttg ggtgggcggc gcgccggccg cgatttgcgc acccaccggc
     1621 aacagcgtgc gccggtcgag cggcgcgagc gccaccaatt ggagtcgcat cgggtcgctc
     1681 gcggcaggcc gcgaaagcga acgccgcccg acaaaatttg cggccttgcg cagggtgcgc
     1741 gcgaagccga tgtcctgcgg caacgtggtg ccgtcggtgt cggtgccgat gtggatgtag
     1801 cccttttcgg tgcgcatgat ctcgagcgcc tcgatgc
//
LOCUS       DASWZT010000290         4052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_74840, whole genome shotgun sequence.
ACCESSION   DASWZT010000290 DASWZT010000000
VERSION     DASWZT010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74840"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VGE92_07930"
     CDS             complement(<1..288)
                     /locus_tag="VGE92_07930"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY1313790.1"
                     /translation="MGLARTFETAANPAQKPARDPLSALLALGRPLVMGILNVTPDSF
                     SDGGKFIDPAVAIAHAGTMAEQGADILDVGAESTRPYGGMKPVSADEEMARL"
     gene            complement(344..754)
                     /locus_tag="VGE92_07935"
     CDS             complement(344..754)
                     /locus_tag="VGE92_07935"
                     /inference="COORDINATES: protein motif:HMM:NF025589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HEY1313791.1"
                     /translation="MTYPITDIDGIDGEAATILKSVGIRSTGRLLQAAHSVRGRKMLA
                     IKTGFEEKQLLYWANMADRMRVKGIRKEYAELLQAAGVDTVKDLKYRNPGNLAKAMAE
                     ANRKRKMVSMLPSQKVVGNWIKNAKTLELMIRYK"
     gene            complement(807..1163)
                     /locus_tag="VGE92_07940"
     CDS             complement(807..1163)
                     /locus_tag="VGE92_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HEY1313792.1"
                     /translation="MDELIARLVANVGVDRAAAEKSVGIILDFLVKEGPADKVQALID
                     KMPGAQALLQASDAGGGFSMGGLMGAGTKMMGAGLSMGQVQGVTKETIAYAREKAGED
                     AVGEIVGAIPGLSQFV"
     gene            1589..2974
                     /locus_tag="VGE92_07945"
     CDS             1589..2974
                     /locus_tag="VGE92_07945"
                     /inference="COORDINATES: protein motif:HMM:NF006579.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY1313793.1"
                     /translation="MSTLDAVLSRIDQDIDQSVERLFALLRIASVSTDPAYKEHCRTA
                     AEHVAADLRSIGFTADVRPTEGHPVVVGKSNGVSGPRVLFYGHYDVQPVDPLNQWKTP
                     PFEPRIETLEGGRKIIVARGACDDKGQAMTFIEACRAFKAVTGKLPLPITTMIEGEEE
                     CGSAHLFKFVRDNAAEFKLDLALVCDTAMWDANTPAITTSLRGLVYEDVKITCANRDL
                     HSGVFGGAAQNPLRLLSKILAALWDDDGRVTIPGFYDGVKELPADIKADLKSLDLTPE
                     KFLGPIGLKIPAGEKGRMLLEQITTRPTAEINGIIGGYTGEGAKTVIPGQASAKVSFR
                     LVGDQDPQKIRDGFRTFVRARLPSDCSVEFGNFGLAAALQLSFDNPALNKARAVLGEE
                     WGKKAVTVGAGGSIPIVADFKNVLGMDSLLVGFALDDDRVHSPNEKFDLKCYHKGIRS
                     WARILAALADA"
     gene            2978..>4052
                     /locus_tag="VGE92_07950"
     CDS             2978..>4052
                     /locus_tag="VGE92_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEY1313794.1"
                     /translation="MAPPLLQLRDIALTFGGTPLLTGAELSVSTGERVSLVGRNGSGK
                     STLLKIAARLVEPDRGSVFVQPGALVRYLPQEPDFAGFASTLAYVEAGLKPTDDHYVA
                     RHLLQELGLTGHEDPAHLSGGEARRVSLARVLAPSPDILLLDEPTNHLDLTTIEWLER
                     DLDARRTALVIISHDRRFLSTLSRSTVWLDRGCARRIERGFGHFEEWRDMLLAEEERE
                     QHKLDRKIVAEEHWMRYGVTGRRKRNMRRVGNLQALREARRGYRATAGDATITAGAAA
                     PSGVLVIEAKGIGKSYAGRPVVSDLSIRIQRGDRIGIIGPNGSGKTTLINMLTGNLPP
                     DAGSVKLGSTLAVATLNQHRDSLD"
BASE COUNT          740 a         1293 c         1254 g          765 t
ORIGIN      
        1 gagccgcgcc atttcctcgt ccgccgacac cggcttcatg ccgccgtaag gtcgcgtcga
       61 ttccgcgccg acgtcgagaa tatcggcgcc ctgttcggcc atcgtgccgg cgtgcgcaat
      121 cgcgaccgcc gggtctatga acttgccgcc gtcggaaaac gaatccgggg tgacattgag
      181 aatgcccatg accagcggcc ggccaagcgc cagcagcgcg gaaagcggat cgcgcgcggg
      241 cttttgcgcg gggttggcgg cggtctcaaa agtgcgggcg agccccatgg cggtcgcttt
      301 gcgctggagg cgcgaggctg tcaagcgcac ccgcatcgcg cggttacttg tagcggatca
      361 tcagctcgag cgtcttggcg ttcttgatcc agttgccgac gactttctgc gacggcagca
      421 tgctgaccat cttgcgcttg cggttggcct ccgccatcgc cttggcgaga tttcctgggt
      481 tgcgatattt gagatccttc acggtatcga cgccggcggc ttgcagcagc tcggcatatt
      541 ccttgcggat gcctttgacg cgcatacggt cggccatatt ggcccagtac agcagttgct
      601 tttcttcgaa cccggtcttg atggcgagca ttttgcgccc gcgtacggag tgcgctgcct
      661 gcagcagccg tccggtcgag cgaatcccca ctgacttgag aatcgtggcg gcttcgccgt
      721 cgattccgtc gatatcggtg atgggataag tcattgagga gcccctgctt gcttcttagc
      781 tcagtctacg tagccgccga aaacgttcaa acgaactgac tcaatcccgg aatcgcgccg
      841 acgatctctc cgaccgcgtc ttccccggcc ttttcgcggg cataggcgat tgtttctttg
      901 gtaacgccct gcacctggcc catgctcagc ccggcgccca tcattttggt gccggcgccc
      961 atcaagccgc ccatggaaaa cccgccgccg gcgtcgctgg cctgcaacag cgcctgggcg
     1021 ccgggcattt tgtcgataag ggcctggacc ttgtcggccg ggccttcctt gaccaggaag
     1081 tcgaggataa tgccgacgga tttttcggct gctgcccgat cgacgccgac atttgcgacg
     1141 agacgcgcga tcaattcatc catgatcccc tcggctctcc cgcactggtg gcgggttgtc
     1201 gttatattgg acgccgtcgg cggcgatgac aagtatgttg caacgtcgtg cgagaaaatt
     1261 tcgcgctgtg cgaacgcact gtcgcgcgcg catgtcaaga aaaatgtaat tcgcagcacg
     1321 ccggcgttcg gcggatcgaa aagcgcaacg cgatacgctc gacagtatcc ggcgaatgtc
     1381 gatcagtcat aattccgggc gcgggagcca tacccagcgg ctattcttac gaaattctga
     1441 aaagaaaatg gaaaattcag gctggaaaac gcaagaccgc cgtttcaact tgcaattcga
     1501 ccgcaattcc gcgtctttgt gcggatcgcg gtgcttttca gcacacgaat gcgttgctaa
     1561 cgtcttgatc ctaccggcag gagcccccat gagcacactc gacgcggtct tgagccgcat
     1621 cgaccaggat atcgaccaaa gcgttgagcg attgttcgcg ctgctgcgca ttgcctcggt
     1681 ttccaccgac ccggcctaca aggaacactg ccggaccgcg gccgagcacg tcgcggccga
     1741 tctgcgcagc atcggattca ccgccgacgt ccgcccaacc gaaggtcatc cggtggtggt
     1801 gggcaaatcg aatggggttt cgggcccgcg cgtgctgttt tacggccact atgacgtcca
     1861 gccggtcgac ccgctgaacc agtggaagac gccgccattc gaaccgcgca ttgaaaccct
     1921 ggaaggcggc cgcaagatca tcgtggcgcg cggcgcctgc gacgataaag gccaggccat
     1981 gaccttcatc gaggcctgtc gcgccttcaa ggcggtcacc ggcaagctgc cgctgccgat
     2041 taccaccatg atcgagggcg aggaggagtg cggctcagcg catctgttca aattcgtgcg
     2101 cgacaacgcc gccgagttca aactcgacct cgctttggtc tgcgataccg cgatgtggga
     2161 cgccaacacg ccggcgatta ccacctcgct gcgcggactg gtctatgagg acgtcaaaat
     2221 tacatgtgcg aacagggatc tgcactccgg cgtattcggt ggcgcggcgc aaaacccgct
     2281 ccgcctgctg tccaaaattc tggccgcgct gtgggatgac gatggccgcg tcaccattcc
     2341 cggtttctac gacggcgtga aggaattgcc ggccgacatc aaggccgacc tcaagtcact
     2401 cgatctcaca ccggagaaat tccttgggcc aatcgggctg aaaatcccgg ccggcgaaaa
     2461 aggccgcatg ctgcttgagc agatcaccac gcggccgacc gccgagatca acggcatcat
     2521 cggcggctat accggggagg gggccaagac cgtcattccc gggcaggcat ctgcgaaagt
     2581 gtcgttccgt ctggtcggcg accaggaccc gcaaaaaatc cgcgacggtt tccgcacttt
     2641 tgtgcgcgcc cgcctgcctt ccgactgcag cgtcgagttc ggcaatttcg gtctggcggc
     2701 ggcgctgcag ctgtcgttcg acaatccggc gctcaacaag gcgcgcgccg tgctcggcga
     2761 agagtggggc aagaaggcgg tgacggtcgg cgccggtggc tcgatcccaa tcgtggcgga
     2821 tttcaagaac gtgctgggga tggactcgct cctggtcggt ttcgcgctcg acgacgaccg
     2881 ggtgcactcg ccgaacgaaa agttcgacct caaatgctac cacaaaggca tccgctcctg
     2941 ggcgcgcatc ttggcggcgc tggcggacgc ttagagggtg gctcctccgc ttcttcaact
     3001 cagggacatt gcactgacgt ttggcggcac gccgctgctc accggcgcgg agctatccgt
     3061 ctcgaccgga gagcgcgtgt cgctggtcgg gcgcaacggt tccggcaaat cgacactgct
     3121 gaagattgcc gcccggctgg tcgagccgga ccgaggctcg gttttcgtgc agccgggcgc
     3181 gctggtgcgt tatctgccgc aggagccgga cttcgccggc tttgcgtcga cgctggccta
     3241 tgtcgaagcc ggcctcaagc cgaccgacga tcactacgtt gcccgtcatc tgttgcagga
     3301 gttgggactc accggccatg aagatcccgc ccatctttcc ggcggcgaag cacgccgcgt
     3361 ctcgctcgcg cgcgtgctgg cgccgtcgcc cgatatcctg ctgctcgacg aaccgaccaa
     3421 ccacctcgac ctcaccacca tcgaatggct ggagcgcgat ctcgatgcgc ggcgtaccgc
     3481 actggtgatc atcagccatg accggcgctt tctctcgacg ctgtcgcgct cgacggtgtg
     3541 gctcgaccgc ggctgcgcgc gccgcatcga acgcggcttt ggccatttcg aggaatggcg
     3601 cgacatgttg ctggcggaag aggagcgcga acagcacaag ctcgaccgca agatcgtcgc
     3661 cgaggaacac tggatgcgtt acggcgtcac cgggcggcgc aagcgcaaca tgcgccgggt
     3721 cggcaatctg caggcgctgc gcgaggcacg ccgcggttac cgcgccacag ccggcgatgc
     3781 cacgatcacc gcgggcgccg cggcgccgtc cggggttctg gtcatcgagg cgaaaggcat
     3841 cggcaagagt tacgccgggc gtccggtcgt cagcgacctt tcaatccgga ttcaacgcgg
     3901 cgaccgcatc ggcatcatcg ggccgaacgg cagcggcaag acgacgctga tcaacatgct
     3961 gacgggaaac cttcctccgg atgccggatc ggtaaagctc ggatcgacgc tcgcggtcgc
     4021 tacgctcaat caacatcgcg acagtctcga cc
//
LOCUS       DASWZT010000291         1610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_79730, whole genome shotgun sequence.
ACCESSION   DASWZT010000291 DASWZT010000000
VERSION     DASWZT010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79730"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VGE92_07955"
     CDS             complement(<1..223)
                     /locus_tag="VGE92_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313795.1"
                     /translation="MRANCSTPLQPRCGAFVSRTDHRNFGRVVQRIQDRQIALAWYTE
                     DAIHALQAQRVHQDLTARLLSRVVFRCGGV"
     gene            373..855
                     /locus_tag="VGE92_07960"
     CDS             373..855
                     /locus_tag="VGE92_07960"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY1313796.1"
                     /translation="MPQQVLIHVHPDPDELGRVYKPQLAIACGVCDFSGALASVESLA
                     PGKRTPWLREAREAYVKFSTPPPPAEHFVDRSAVVGWLSEHLADDGCFLMYPQEIATA
                     VQQQANLVIIIVNNAMYGTIRMHQENRFPGRAVDSGRPGVLELLVDPAEISPMFRLLV
                     "
     gene            873..>1610
                     /locus_tag="VGE92_07965"
     CDS             873..>1610
                     /locus_tag="VGE92_07965"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY1313797.1"
                     /translation="MTGRTTGILIGMSATSCKCLVIHSPLDLRVVEIDAGEVGCGQVA
                     VRVAIGGICGSDLHYFHNGGFGTVRIKRPMILGHEVAGTVVSVGPDVTAVKIGDRVAV
                     NPSRPCGTCSYCLEGLPNQCLEMRFYGSAMRDPHVEGAFRNLLVCDAVQCAALAAHVP
                     FEIASLAEPFSVALHGVARAGSLLNKRVLVSGCGPIGTLAIAAAFAHGAMEIVAVDVT
                     DETLIIAREMGATKTINVATRPDWTQQY"
BASE COUNT          284 a          465 c          525 g          336 t
ORIGIN      
        1 caaccccacc gcagcgaaac acaactcgac tcaacagacg ggcggtcaag tcctggtgga
       61 cgcgctgcgc ctgcaaggcg tggatcgcat cttctgtgta ccaggcgaga gctatttgcc
      121 ggtcttggat gcgctgtacg acgcgcccga aattgcggtg gtcagtgcgc gacacgaagg
      181 cgccgcagcg cggctgcaac ggcgtgctgc agttcgcgcg caagaacgag ctgccgatag
      241 ttgcgagttt tcgtcgtcag gatctcatcg acaaccgcga tccgaattat tgcggatact
      301 tagggctcgg tgtcgacccg aacctcgcgg aacgcgttcg ctgcgccgat ttgatcgctg
      361 ctgctgccgc cggtgccgca gcaggtgctg attcacgttc atcccgatcc cgacgaattg
      421 gggcgtgttt ataagccgca attggcgatt gcatgcggcg tctgcgattt tagcggcgca
      481 ttggcaagcg tcgagagtct ggcgccgggc aagcggacgc cgtggctacg cgaagcgcgc
      541 gaggcttacg tcaagttcag cacgccgccg ccgccggcgg agcactttgt cgacaggtcc
      601 gctgtggtgg gctggttgag cgagcatctc gccgacgacg gttgttttct gatgtatccg
      661 caggagattg cgaccgccgt tcaacagcag gccaatttgg tgatcattat tgtcaacaac
      721 gcaatgtacg gcaccatccg aatgcatcag gaaaaccgct ttccgggccg cgcggtcgac
      781 tccgggcgac cgggggtgct cgaactactg gtcgatcctg ccgagatttc gcccatgttc
      841 aggctgttgg tgtgaggcat gagaccggtc ccatgaccgg ccgaactacc ggtatactca
      901 tcgggatgag cgcgacaagt tgcaaatgct tggtcatcca ttcgccgctg gacctgcgcg
      961 tcgtggagat cgatgcgggt gaggtgggtt gcgggcaggt tgcggtgcgc gtcgctatcg
     1021 gcggtatctg cggctctgac ttgcattatt ttcataacgg tggctttggg acggtgcgca
     1081 tcaagcggcc gatgattctg ggccatgagg tcgccggcac cgtggtttcg gttggccccg
     1141 atgtaaccgc agtgaagatc ggcgatcgcg tcgccgttaa tcccagccgg ccgtgcggta
     1201 cctgcagcta ctgcttggag ggtctgccaa accaatgtct cgagatgcgt ttctatggca
     1261 gcgcgatgcg cgatccgcat gtcgaaggag cgttcaggaa tttgctcgtg tgcgacgcgg
     1321 tgcaatgcgc ggcgctggcg gcgcatgttc cgttcgagat cgcctccttg gcggagccat
     1381 tttcggttgc cttgcacggg gtggcacgtg ccggatccct cctgaacaag cgcgtactcg
     1441 tttcgggctg tggacccatc ggcacgctgg cgatcgctgc cgcctttgcc catggcgcca
     1501 tggaaatagt cgccgtggac gtgaccgacg aaaccttgat catcgcccgc gagatgggtg
     1561 cgaccaagac gatcaatgtc gcgacccggc ccgactggac gcagcaatac
//
LOCUS       DASWZT010000292         6873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_60371, whole genome shotgun sequence.
ACCESSION   DASWZT010000292 DASWZT010000000
VERSION     DASWZT010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6873
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60371"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VGE92_07970"
     CDS             <1..267
                     /locus_tag="VGE92_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313798.1"
                     /translation="FELRDAFSAAPLGEYDVVAVTGSLPVYDTRFEKALRVGGRLFAI
                     VGVAPVMDAVLVRRVDNDEWIRESLFETVVDALINATAAQKFVF"
     gene            267..590
                     /locus_tag="VGE92_07975"
     CDS             267..590
                     /locus_tag="VGE92_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY1313799.1"
                     /translation="MIKDMSPKEFVERRASGGTMTLLDVREDWETTLAPVPADLVHIP
                     MGQVADRLGELDPQQETVVICRSGGRSREVAHFLSTQGFVSVYNLAGGILAWSRDLDP
                     RIPQY"
     gene            664..2106
                     /locus_tag="VGE92_07980"
     CDS             664..2106
                     /locus_tag="VGE92_07980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEY1313800.1"
                     /translation="MKRFLTLLALSLAASQAPAADLLAVYQRALQNDPQLREAEATRL
                     AALEAKPQALSALLPQLSASGTVTREKDTGSTNTTEPVSTGPSLTSPTVLESFPFAGR
                     VDTTTHRYGLDLKQNIFRWENWVALQRADSQVAQAEADYQAAQQDLMERVAQRYFDVL
                     AAQDDLEAQQVALLSIQRQLDQAESRFQIGLIAVTDVEEARAAHDSGAAAVIAAKRTL
                     ASTQELLREITGDGFDWLARPIEPFELSNPDPVSEDRWVEMALQQNLSLVSSRLAADI
                     ARENVSSARGGHFPSLDLVGSHYKATSNGTDTFNDGMPAGGTTLDETQRTIGLQLTFP
                     IYSGGMVSSQVRQAVYQHRAAKERVERVARQTEHDARDSFLGVLSEISRVKALRRAVE
                     SNAISLRATESGYEAGTRTAVDVLQSRQLWVQAQTDYARSRYDYMLNVLKLQQAAGTL
                     SQQSLEKINSLLKDAPPPASATPDPAAAAR"
     gene            complement(2036..3358)
                     /gene="waaA"
                     /locus_tag="VGE92_07985"
     CDS             complement(2036..3358)
                     /gene="waaA"
                     /locus_tag="VGE92_07985"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF004388.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEY1313801.1"
                     /translation="MMRRLYSVLIFCLAPFAFAVVLWRGVRDRGYWQGLPERFGWGAK
                     LDGGPTIWLHAVSLGEMSAAAPLVRALRTRYPQYALVLTTGTPTGRARARGLFIDADV
                     RFVPYDTPSAVSRFLDLTRPTLAIIMETELWPNLFQECERRGVPVVLASARLSAKSVA
                     RYVRFGDLFRGIFSDNLLLAAQTQEDAERFIAIGAESARTHLVGNIKFDLELSPGVID
                     RGHELRAAFGANRPVWIAGSTHAGEEEQVLAAHAQLRGAKPEALLLLVPRHPERFANV
                     ADLLRRQNLRFVRRSLGAANGAAPAGEAHVVLVDTVGELAALYAAADVAFVGGSLVPI
                     GGHNLLEPAALGLPVLTGPSYTNGKDIARLLLKQGAALQVANAAELAAALRSLLSDPA
                     ARQRMGASGRQIVAANRGSVRRLLSLIEPLLPDPASLKPAAARPSTGC"
     gene            complement(3355..4044)
                     /locus_tag="VGE92_07990"
     CDS             complement(3355..4044)
                     /locus_tag="VGE92_07990"
                     /EC_number="2.7.1.166"
                     /inference="COORDINATES: protein motif:HMM:NF002475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="HEY1313802.1"
                     /translation="MLSDPACIGNLPQDAQESLFDPKFWRARGELIAVTGGRGSAWFI
                     ASGARQWALRHFRRGGCIARVSQDGYVWAGENKVRAFREWRLLELLAQRGLPVPKPVA
                     ARYSRTGLLYRCDLITQRIMAAEPLSAVIAARALPESLWHGVGATVARLHRAGVDHAD
                     LNAHNLLLDVRGQISVIDFDRGRIRTPGAWRARNLQRLRRSLFKVASGLPAGRFSEAA
                     WSALLAGYQTG"
     gene            4089..5153
                     /locus_tag="VGE92_07995"
     CDS             4089..5153
                     /locus_tag="VGE92_07995"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY1313803.1"
                     /translation="MTLDFNPPPQHVCLLRLSAIGDTCHALAALRAFQAAWPQTRFTW
                     IIGRLEAKLMTAVVPEIEFITFDKSATLGELRRLRAVLRGRRFDLLLDLQLAFRASLV
                     STFIDAPLKLGFDRKRARELQWLFTNARIAPAAAEHVLDSFMGFARACGINPQQPFWN
                     LALPPQAIDYAREIVSDERPTLLISPCSSHPTRNWSAERYAAVADHAAAAHRMRIVLV
                     GGRSTIEAQMGSHIMAAGRPGIINQIGKDTLPQLLGLLSRSSVLLSPDSGPVHMATMV
                     GLPVIGLYAATNPARAGPYYSRQWCVDKYDEAARRFLRRPSADIPWTTKIERSGVMDL
                     IAASEVTAKLDALMAAGVFR"
     gene            5164..5559
                     /locus_tag="VGE92_08000"
     CDS             5164..5559
                     /locus_tag="VGE92_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313804.1"
                     /translation="MNEMKIPISPGEVLDKITILRIKTQRMSDPAKLANVRAELRALE
                     ATWAESAFAKVNIDADVDALLAVNERLWVIEDDIRDKERAQSFDAEFIRLARAVYFEN
                     DERAAIKRRINIALGSGLVEEKSYRDYKT"
     gene            complement(5578..5829)
                     /locus_tag="VGE92_08005"
     CDS             complement(5578..5829)
                     /locus_tag="VGE92_08005"
                     /inference="COORDINATES: protein motif:HMM:NF021772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEY1313805.1"
                     /translation="MPAMVQDDSKHQLIQPNALHQYTVTAADLPLSCPMPGMFLWNSH
                     PKVYLAIEATGWAKCPYCGAEYTLKREGVPEGAREGVVA"
     gene            complement(5859..>6873)
                     /locus_tag="VGE92_08010"
     CDS             complement(5859..>6873)
                     /locus_tag="VGE92_08010"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional glutamine synthetase
                     adenylyltransferase/deadenyltransferase"
                     /protein_id="HEY1313806.1"
                     /translation="VEALRQFQKVAVFSVALADLTGRLPLMKVSDRLTDIAELIVQCC
                     VDLAWQQMTAACGIPYCGDDEQHLRRVTVAVVGYGKLGGLELGYGSDLDLVFLHDSRG
                     EIQRTRGERPLDNGIFFLRLGQRIVHLLTMHSAAGRLYEVDMRLRPNGKGGFLITGID
                     AFERYQRQDAWTWEHQALLRARPVAGDTNLREAFEAARRRVLGVAVHRDTLRTDVKEM
                     RLRMRRELSKARAGQFDIKQDAGGIADIEFLVQYRVLAAANAHPVLLTHSDNIRQLEG
                     LAAAGVLEPATAQWLTDAYIGYRSVLHHLSLEGPGERVVDAAPYAATRARVVEIWRAT
                     FDT"
BASE COUNT         1297 a         2270 c         2118 g         1188 t
ORIGIN      
        1 ttcgagctcc gcgatgcctt cagcgcggcg ccgctgggcg aatacgacgt cgttgccgtc
       61 accggctcgc tgccggtgta tgacacccgc ttcgagaagg cgctgcgtgt cggcggccgc
      121 ctgttcgcga tcgtcggcgt cgcgcccgtc atggacgccg tgctggtacg ccgtgtcgac
      181 aacgacgagt ggattcgcga gagcctgttc gagaccgtcg tcgacgccct gatcaatgcc
      241 accgccgcac agaagttcgt gttctgatga tcaaagacat gagccccaag gaattcgtcg
      301 agcggcgcgc gagcggcggc acaatgaccc tgttggacgt acgcgaggac tgggaaacga
      361 cgctggcgcc ggtgccggcc gaccttgtgc acattccgat gggccaggtg gcggaccgtc
      421 tcggcgaact cgacccgcag caggagacgg tggttatctg ccgttccggc ggccgcagcc
      481 gcgaggtggc gcacttcctc tcgacccaag gtttcgtttc ggtgtacaac ctcgccggcg
      541 gcattctggc ctggtcccgg gacctcgatc cacgaatacc gcaatattga tctatcgggc
      601 tcttgactcc attggtgata tggttatata taacaccata tgaccctctg gcgatcgaat
      661 aatatgaagc gattcctgac cctattggcg ttgagtttgg ccgcgtccca ggcgccggct
      721 gctgatcttc tggcggtgta tcagcgcgcg ctgcaaaacg atccgcaact gcgcgaggca
      781 gaggccactc gtctggcggc gctcgaggcc aagccgcaag ccttgtcggc cctgctgccg
      841 caattgtcgg ccagcggcac cgtgacccgc gagaaggaca cgggctcgac caataccacg
      901 gaacctgtgt ccacgggccc gagcctcacg agcccaacgg tgctcgaaag ctttccgttc
      961 gccggtcggg tcgacaccac cacgcatcgc tacgggctcg atctcaagca aaacattttt
     1021 cgctgggaga attgggtggc cctgcagcgc gccgactccc aggttgcgca ggccgaggcg
     1081 gattatcagg ccgcgcagca ggatctcatg gagcgcgtcg cgcagcgcta cttcgacgtg
     1141 ctcgccgcgc aagacgacct cgaggcgcaa caggtcgcgc tgctttcgat ccaacggcag
     1201 ctcgaccaag cggagtcgcg ctttcaaatc ggcctcattg ccgtcaccga tgtcgaggag
     1261 gcgcgtgcgg cgcacgacag cggcgcggcg gctgtgattg ccgcaaagcg taccctcgcc
     1321 tccacccagg agctgctgcg cgaaatcacc ggcgatggct tcgactggct ggcgcgtccg
     1381 atcgagccgt tcgagctgtc caatcccgat cccgtgagtg aagatcgctg ggtcgaaatg
     1441 gccttgcagc agaatctgtc gctggtatcg agtcggttgg cggccgacat cgcgcgcgag
     1501 aacgtgagct cggcgcgcgg cggccacttt ccgtcgctcg atttggtggg cagccactac
     1561 aaggcgacca gcaacggtac ggatacgttc aacgacggta tgcccgcggg cggcacgacc
     1621 ttggatgaaa cacaacgcac gatcggcctg caattgacct tccccatcta ctcgggaggc
     1681 atggtctcgt cccaggtgcg tcaggcggtc taccagcatc gcgcagcaaa agagcgcgtg
     1741 gagcgggtag cgcgtcagac cgagcacgac gcgcgcgatt cctttctcgg cgtattgtcg
     1801 gagatttcgc gggtaaaggc gctgcgccgg gcggtcgagt cgaacgccat ctcgctgcgc
     1861 gccaccgagt ccggttatga ggcgggcact cgcaccgcgg tggacgtgct gcagtcgcgt
     1921 cagctctggg tgcaggctca gactgactat gcccgcagcc gctacgacta catgttgaat
     1981 gtgctcaaac tgcagcaagc agccggtacg ctgtcgcagc agagtttgga aaagatcaac
     2041 agcctgttga aggacgcgcc gccgccggct tcagcgacgc cggatccggc agcagcggct
     2101 cgatgaggct gagcaagcgc cgcacgctgc cgcgattcgc tgcgacgatt tgcctgccgc
     2161 ttgcccccat gcgctgacgc gcggccggat cgctcaacag cgagcgcagc gccgctgcaa
     2221 gctccgcggc attcgccact tgcaaggccg cgccctgctt cagcaacagc cgcgcaatgt
     2281 ccttgccgtt cgtataggaa ggacccgtca agaccggcag cccgagcgcc gcgggctcca
     2341 acaagttatg accgccgatc ggcaccagac tcccgccgac gaacgcaaca tcggccgcgg
     2401 catacagcgc ggcgagctca cccaccgtgt ccaccagcac cacgtgtgcc tctcccgcag
     2461 gcgccgcgcc gtttgccgcg ccgaggctgc ggcgcacgaa gcgtagattt tggcgccgca
     2521 gcaggtcggc cacgttagcg aagcgctccg gatgtcgcgg caccagcaac aaaagagctt
     2581 cgggcttcgc tccgcgcaat tgcgcatgcg cagccagcac ctgttcttcc tcgcctgcat
     2641 gagtgctgcc ggcaatccat accggccgat tggcgccaaa ggcagcgcgc aactcatgcc
     2701 ctcgatcgat cacccccgga ctcaattcga gatcgaactt gatgtttccg accaggtgag
     2761 tgcgggcact ctcggcaccg atcgcaatga aacgctcggc atcttcctgg gtctgagccg
     2821 ccagcagcaa attgtccgag aatattccgc gaaacaagtc gccgaacctc acgtagcgcg
     2881 caacggactt tgccgataat cgcgcactcg cgagcaccac gggcacgccc cgccgctcgc
     2941 actcctggaa cagattcggc cagagttcgg tttccatgat gatggccagc gtcggccggg
     3001 tgagatccaa gaagcgggat accgcactcg gcgtgtcgta cggcacgaaa cgcacatccg
     3061 catcgatgaa caagccgcgc gcgcgtgccc ttccggtcgg tgtcccggtg gtcaacacca
     3121 gcgcgtattg cgggtaacgc gtgcgcagcg cgcgcaccag cggtgcggcc gccgacatct
     3181 cacccaaact cactgcatgc agccagatgg tcgggccgcc gtcgagcttg gcgccccagc
     3241 cgaaacgctc ggggagaccc tgccagtagc cgcggtcgcg cacgccgcgc cacaacacca
     3301 cggcaaatgc gaacggcgcg aggcagaaga tcagcaccga gtataggcgg cgcatcatcc
     3361 agtctggtat ccggcgagca gcgcactcca ggcggcctcc gaaaatcggc cggccggcaa
     3421 gccgctcgct accttgaaaa gcgagcggcg caagcgctgc aaattgcgcg cgcgccacgc
     3481 gcccggcgtg cgtattcggc ctcgatcgaa gtcgatcacg ctgatctggc cgcgcacatc
     3541 gaggagtaag ttgtgggcat tgagatcggc atgatcgaca cccgcgcgat gcaggcgggc
     3601 gacggttgcg ccgacgccgt gccacagcga ctcgggcagc gcacgcgccg cgatgacggc
     3661 gctcaaaggc tcggccgcca tgatccgctg cgtgatcaaa tcgcagcggt acaacaagcc
     3721 ggtgcggctg taacgcgcag ccacgggttt cggcaccggc aggccacgtt gcgccagaag
     3781 ctcgagcagc cgccactcgc ggaaagcccg cactttgttt tcgcccgccc aaacgtagcc
     3841 gtcctgcgag actcgcgcaa tgcatccgcc gcggcggaag tgccgcaatg cccattgacg
     3901 agcgcccgag gcgatgaacc aagccgaccc gcggccgccc gtcactgcaa tgagctcgcc
     3961 gcgcgcacgc caaaatttcg gatcgaacaa cgactcttga gcatcctgcg gcagattgcc
     4021 aatgcaagcc ggatcagaca gcatggcgcc cgtggcagtg gcaatgcgtc ggccgtttat
     4081 cactttatat gactcttgat ttcaacccac cgccgcagca tgtgtgtctg ttgcgtctct
     4141 cggctatcgg cgatacctgc catgcgctgg cagcgcttcg agccttccag gcggcgtggc
     4201 cgcaaacccg cttcacctgg atcatcggca gactcgaagc caaactgatg acggccgtcg
     4261 ttccggaaat cgagtttatc accttcgaca aaagcgcaac gctcggcgag ttgcggcgac
     4321 tgcgcgcggt gttgcgcggc cggcgattcg atttgctgct cgatttgcaa ctggcgtttc
     4381 gcgcgagtct ggtgagcacg ttcatcgacg ctccgctcaa gctcggattc gaccgcaaac
     4441 gtgcacgtga gcttcaatgg ttgttcacca atgcgcgcat cgcgccggcg gcggccgagc
     4501 acgtactgga ttcgttcatg ggtttcgcgc gcgcctgcgg catcaacccc cagcagccgt
     4561 tctggaatct ggcgctgccc ccgcaggcca tcgattacgc ccgcgagatc gtttccgacg
     4621 aacgcccaac cctgctgatc agcccctgct ccagtcatcc gacgcgcaac tggtcggccg
     4681 agcggtatgc cgccgtggcc gaccacgcag ccgccgcgca ccgtatgcgc atcgtattgg
     4741 tcggcggccg cagcacgatc gaagcgcaaa tgggttcgca catcatggcc gcgggccgcc
     4801 ccggcatcat caatcaaatc ggcaaggaca ccttgccgca gcttctcggg ttgctgtcgc
     4861 gaagcagcgt gctcctgtcc ccggattcgg gccccgtgca catggccacg atggtggggc
     4921 tgccggtgat tggcctgtat gcagccacca atccggcacg agccggtccg tactacagcc
     4981 ggcaatggtg tgtcgacaaa tatgacgagg cagcgaggcg ctttttgcgc aggccttcgg
     5041 cggatattcc ctggaccacc aagatcgagc gcagcggcgt catggatctg atcgccgcga
     5101 gcgaggtgac ggcgaaactc gatgcgctga tggcggccgg cgtttttcgc taacataggg
     5161 cgcatgaacg aaatgaaaat cccgatttcc ccgggcgagg tgctcgataa gataacgatc
     5221 ctgcgcatca agacgcagcg catgagcgac ccggccaagc tcgccaatgt ccgcgcggaa
     5281 ttgcgcgcgc tcgaggcgac gtgggccgag tcggccttcg ccaaggtcaa tatcgacgcc
     5341 gacgtcgatg ccttgttggc cgtcaacgag cgcctctggg tcatagagga cgacatccgc
     5401 gacaaggagc gcgcgcagtc cttcgatgcg gaattcatcc gcctggcgcg cgcggtttat
     5461 ttcgagaacg acgaacgtgc cgccatcaag cggcgcatca acatcgcatt gggctcgggt
     5521 ctcgtcgaag aaaaatcata tcgcgattac aagacttgag cgcaacgccg cggtgagtca
     5581 agcgacaacg ccttcgcgag cgccttcggg aacgccttcg cgtttgagcg tgtactcggc
     5641 gccgcagtac gggcacttcg cccagccggt ggcttcgatc gccagataaa ccttggggtg
     5701 cgagttccac agaaacatgc ccggcatcgg acacgacagc ggcaaatccg ccgcggtcac
     5761 ggtgtactga tgcagcgcgt tcggctgaat caattgatgt ttcgaatcgt cttggaccat
     5821 cgccggcatt gttcgctccg ctgcatattc cgaatcattc acgtgtcaaa cgtcgcgcgc
     5881 catatctcaa cgacgcgcgc gcgggtcgct gcgtagggtg ccgcgtccac gacgcgttcg
     5941 ccagggcctt ccagcgacag gtgatgcagc acgctgcggt agccgatgta ggcatccgtc
     6001 agccactgcg ccgtcgccgg ttcgagcaca cccgccgcgg ccaatccttc gagctgccga
     6061 atattgtccg aatgggtcag tagcacgggg tgtgcattgg ccgccgccag cacccggtac
     6121 tgtacgagaa actcgatgtc cgcgataccg ccggcatctt gcttgatgtc gaactggccg
     6181 gcgcgcgcct tggagagttc gcggcgcatc cgcaggcgca tctccttgac gtccgtacgc
     6241 agcgtgtcgc gatgcacggc cacgccgagc acgcggcggc gtgccgcctc gaaggcttca
     6301 cgaagattcg tatcgcccgc caccggccgt gctcgcagca gcgcctggtg ttcccaggtc
     6361 caggcgtcct ggcgctgata gcgctcgaat gcatcgatgc ccgtaatgag aaagccgcct
     6421 ttaccattcg gccgcagccg catgtccacc tcgtacaggc ggccggccgc cgaatgcatg
     6481 gtgagcaggt gcacaatgcg ctggcccaat cttaaaaaaa agatgccgtt gtcgagcggc
     6541 cgctcgcccc gggtgcgctg aatctcgcct ctcgagtcgt gcaggaacac cagatcgaga
     6601 tcggagccgt aaccgagctc caaaccgccc aatttaccgt agccgacaac ggcaacggtc
     6661 acccggcgca agtgctgttc gtcgtcgccg cagtaaggga tgccgcaagc cgcggtcatc
     6721 tgttgccagg ccagatccac gcagcattga acgatgagtt cggcaatatc ggtcagccgg
     6781 tcgctcactt tcatcagcgg caagcgcccg gtgagatctg cgagcgccac actgaacacc
     6841 gcgactttct ggaactgccg cagagcttcc acc
//
LOCUS       DASWZT010000293         2161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_45917, whole genome shotgun sequence.
ACCESSION   DASWZT010000293 DASWZT010000000
VERSION     DASWZT010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45917"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VGE92_08015"
     CDS             complement(<1..524)
                     /locus_tag="VGE92_08015"
                     /inference="COORDINATES: protein motif:HMM:NF013396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEY1313807.1"
                     /translation="MPLAKVAEFVGASGKFVPEAIAQGYSIDSRGIASGQLFFAVKGE
                     RLDGHDFVEQALEKGAVAAVVRKDQLNRYADEKRLLAVEDTLVALQTLATAVRKLWGK
                     PLIAITGSAGKTTTKDAIAHVLRTRFRVLKSEGNFNNHFGLPLMLLKLEPEHDLAVIE
                     MGMSHAGEIRALAK"
     gene            complement(527..2038)
                     /locus_tag="VGE92_08020"
     CDS             complement(527..2038)
                     /locus_tag="VGE92_08020"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEY1313808.1"
                     /translation="MTFQQLLDGAEVLAHSGNPDVSSVEYDSRRVQPGCLFVALRGES
                     SDGNQFIDPAIRAGAVAVVTDSAAQSQRPGVAWAVVARGRRALARVSANFYKRPAERI
                     AITGITGTNGKSTTAFLLESILQAAKRKSALIGTIEYHVAGKILPAPHTTPEALELNR
                     IFSEALGAGATDAVMEVSSHALAQERVYGVPFDVAVFTNLTRDHLDYHKTMEEYFAAK
                     RALFEGCGTDPSRAVVTNLDDEYGAKLAEFSRKRSSLVLKYGWQQGDLRAQKVEITTR
                     GTRFDLIAPGQQIALFSALIGRVNVYNILAAAGAAVARGCTAEAIAQGVGNLACVPGR
                     FERVDCGQPFTVVVDYAHTDDALCNLTALARELVSASKGRVLTMFGCGGDRDRKKRPL
                     MGEAAGRGSDLVVLTSDNPRSEDPLAIINDAVVGLQKTGAKYSVEPDRRKAIALAISE
                     ARPGDIVLLAGKGHEKVQVTRGRAAPFDDLEVARQALQAVGFECGAAAAGAPK"
BASE COUNT          384 a          715 c          646 g          416 t
ORIGIN      
        1 atcttagcca gtgcgcgaat ttctcccgca tgcgacatgc ccatctcgat tacggcgagg
       61 tcatgctcgg gctccagctt cagcagcatg aggggcaggc cgaaatggtt gttgaagttt
      121 ccttccgact tcagcacgcg aaagcgggtg cgcagcacat gggcgatggc atcctttgtg
      181 gtcgtctttc ccgccgaccc ggtgatggcg atcagcggtt tgccccacaa cttgcgcacc
      241 gccgtggcca gagtctgcag cgcgaccagc gtgtcctcga ccgccagcaa tcgcttctcg
      301 tccgcatagc ggttgagctg gtctttccgc accaccgccg caaccgcgcc tttttccaaa
      361 gcctgttcca cgaagtcgtg gccatcgagg cgctcgcctt tcacggcaaa gaaaagctgc
      421 cccgacgcaa tgccgcgcga gtcgatcgaa taaccttgcg cgatagcttc cggcacaaac
      481 ttcccgcttg ctccgacgaa ctccgcgacc ttggccagag gcaacctcat ttgggggctc
      541 cggcggccgc ggcaccgcac tcgaaaccca cagcctgcag cgcctggcgc gcgacctcca
      601 aatcgtcgaa gggagccgca cgtccgcgcg tgacttgtac tttctcgtga ccttttccgg
      661 cgagcagcac aatgtctccc ggacgggctt cggagatggc gagagcgatc gctttccggc
      721 ggtccggctc gacgctgtat tttgcgccgg ttttctgcaa gccaaccacg gcgtcgttaa
      781 taatcgccag cggatcttcg ctgcggggat tgtcggaggt gagcaccacc aaatcgctgc
      841 cacgccccgc ggcctcgccc atgagcgggc gcttctttcg gtcgcggtcg ccgccgcagc
      901 caaacatggt taagactcga cccttcgacg cggacaccaa ctcgcgcgcg agcgccgtca
      961 gattacacag ggcatcgtcg gtgtgcgcgt aatcgaccac cacggtaaaa ggctggccgc
     1021 agtcgacgcg ctcgaagcgc cccggaacgc aggccagatt gccgacgccc tgggcaatcg
     1081 cctccgcggt gcaaccgcgc gcgacggcgg cgccggccgc ggccagaatg ttgtacacat
     1141 tcacgcggcc gatcagcgcg gagaacagcg cgatttgttg gcccggcgca atcagatcga
     1201 agcgcgtgcc gcgggtcgtg atctccactt tctgggcgcg cagatcgcct tgctgccagc
     1261 cgtatttcag cacgagcgag ctacgcttgc ggctgaactc ggccaacttg gctccgtact
     1321 cgtcatccag attcgtaacc acggcgcgcg acggatcggt gccgcagccc tcgaataagg
     1381 cgcgcttggc ggcgaaatac tcttccatcg ttttgtgata gtcgagatgg tcgcgagtga
     1441 gattggtgaa cacggcgaca tcgaagggca cgccatagac tcgctcttgc gccagcgcgt
     1501 gcgaggagac ttccatcacc gcgtcggtcg cgcccgcgcc cagcgcttcg ctgaagatgc
     1561 gattgagttc gagagcttcc ggcgtcgtgt gcggcgccgg caatattttc ccggccacat
     1621 ggtattcgat cgtgccgatc aacgcacttt ttcttttcgc cgcttgcaga atcgattcca
     1681 gcaggaaggc tgtggtgctc ttgccattgg ttcccgtgat gcccgtgatc gcgatgcgct
     1741 cggccggtcg tttgtagaag ttggcgctca cccgagccag cgcgcgccgc ccgcgcgcca
     1801 cgaccgccca ggccacgccc gggcgctgag attgcgccgc cgaatccgtg accaccgcca
     1861 ccgctccggc gcgaatcgcg ggatcgataa actggttgcc gtcgctcgat tcgccgcgca
     1921 gggcgacaaa caggcaaccc ggctgcaccc gccgagagtc gtattccaca ctactcacat
     1981 ccggattgcc gctgtgcgcg agcacttccg ctccatccag caactgttgg aaggtcatta
     2041 cccggaatta tatggtacgg ggagtggcga tcgcggccgg gaatggcggc gtgaacctaa
     2101 ttgaaaagtg catggttact tatggcactt tccccatctt ggcccgggcg ctacattgct
     2161 g
//
LOCUS       DASWZT010000294         4541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_74854, whole genome shotgun sequence.
ACCESSION   DASWZT010000294 DASWZT010000000
VERSION     DASWZT010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74854"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /locus_tag="VGE92_08025"
     CDS             <1..1242
                     /locus_tag="VGE92_08025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY1313809.1"
                     /translation="THDGTPKWWDVIVMPIRDADGNVAKLLAVARDITEIQRAENTLL
                     ETNRFLDSLIENLPLMVFVKDAKSLRFVRQNRASEKLLGIPAGQMLGKSDRDFFPPAE
                     ADSFMARDREALAGGRLVDIPEESIHTQQLGVRTLHTMKLPILDERGLPKFLLGISVD
                     ITERKQAEQAIRKLNAELLENTAQLLATNKELEGFSYSVSHDLRAPLRAIDGFSLMLQ
                     EDCHAMLNDEGRRYVTVIRDNTRRMGALIDDLLAFSRLGRQPVADREINVDSLVREVI
                     EEVVGSAPAPRIEVEPLPSTRGDRGLLRQVWVNLIANAIKYSSKAAQPLIQISGRQVG
                     TENHYSVRDNGVGFNMAYVGKLFGVFQRLHRADEFAGTGVGLAIVHRVVTRHGGRVWA
                     DGEVDRGAVFSFALPNGGSNG"
     gene            1235..1678
                     /locus_tag="VGE92_08030"
     CDS             1235..1678
                     /locus_tag="VGE92_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1313810.1"
                     /translation="MDDFEAVEILLVEDNDADAEMTTRALRKGNVINHVVRLHDGVEA
                     LEFLFREGAFALRRGPQPRLILLDLKMPRLGGIDVLRRLKSDTGTKMIPVVVLTSSAE
                     EKDVVESYQLGVNSYLVKPVEFSAFASVINQTGLYWAVMNRIPNL"
     gene            1808..3316
                     /locus_tag="VGE92_08035"
     CDS             1808..3316
                     /locus_tag="VGE92_08035"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY1313811.1"
                     /translation="MPGKRLVDDLVHRNILGNSLFSRRFKQLTSRLAPYAAALSAALL
                     LWHAARDTPTSVAYAPAPLVYPTAQRAVPAPSPPPPAAAVQSSAPAIAQQANVGATIE
                     VVVGRNDTLDAIFRRMALDKADLAAIRQLPGIRQSLDFLRPGDAIKLKHSDGEIEELT
                     RKVSETQTLKVVREDSGFAAQMINNPVETRIRTASATIDSSLFQAAESADVSDIVALK
                     LANIFAWDIDFVLDIREGDRFTAVYEQIYQDGKYLRDGDVLAAEFVNSGKVYRAVRFA
                     ADGSSASYYSPDGLPLRKAFLRAPVEFTRVSSVFNPHRMHPILNLIRGHMGTDYAAPI
                     GTPVHAAGDGRISFAGPRGGYGNALVLAHSNSVSTLYGHLSRFAKHIRVGTHVQQGEV
                     IAYVGMTGLATGPHLHYEYLVDGVHKNPQTVHLPGAEPLSADMLKKFHATTAPLLANL
                     APQPAPSGSAPTAAFGPLADSTPPVVPLAGSMFGVSAVLSDADALDRPALHD"
     gene            3328..4428
                     /locus_tag="VGE92_08040"
     CDS             3328..4428
                     /locus_tag="VGE92_08040"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEY1313812.1"
                     /translation="MGFYLGLMSGTSMDAIDAALVDFDGSPLRILATSATRFDPALKQ
                     RIAAVLDFPDSVALDEIGQIDVELARAFAAAALALLRQAGVGSGGVAAIGSHGQTLRH
                     RPDLAAPFTWQIGDPNTLSEMTGITVVADFRRRDVAAGGQGAPLLPVFHDQVFRSERE
                     DRVIVNLGGIANITILQRDMPVIGFDTGPANRLLDAWVSLQQGKELDAGGEWAGTGRC
                     DDALLQKLLDEPYFRLAPPKSTGRELFNLAWLNHKLGSAPRRPQDVQATLQQFTAATL
                     AAEVHRHAPGAALYVCGGGAHNAGLLAAIARSVAPSTVATTTALGLDPDYVEAVAFAW
                     FARRTLEGLTSSAASVTGAAGPRILGGVYRYA"
BASE COUNT          866 a         1474 c         1391 g          810 t
ORIGIN      
        1 acgcacgacg gcacgccgaa atggtgggac gtcatcgtca tgccgatacg cgatgccgac
       61 ggcaacgtgg ccaaattgct ggcggtggcg cgcgacatca ccgagatcca gcgcgcggaa
      121 aacacattgc tggaaaccaa ccgcttcctc gattctttga tcgagaatct gccgctcatg
      181 gtcttcgtca aggacgccaa gagcctgcgc tttgtccgcc agaaccgtgc aagcgaaaaa
      241 ttgctgggaa ttcccgccgg acaaatgctc gggaaaagcg accgcgactt cttcccgccg
      301 gcggaagccg attctttcat ggccagggac agggaagccc tcgccggcgg acgcctggtc
      361 gacattcccg aggagtcgat ccacacgcag cagctcggcg tgcgcacctt gcacacgatg
      421 aagctgccga ttctcgacga gcgcgggcta ccgaagttcc tattgggaat ctcggtcgat
      481 atcaccgaac gcaagcaagc cgagcaggcc atccgcaaat tgaacgccga actgctcgag
      541 aacacggcgc aactgctagc caccaacaag gagctggaag ggttcagtta ttcagtctcg
      601 cacgacttgc gtgcgccgct gcgcgccatc gacggctttt ccctgatgtt gcaggaggat
      661 tgtcacgcga tgctcaatga tgaggggcgg cgctacgtca cggtgatccg cgacaacaca
      721 cggcgcatgg gggcgctcat cgacgatctg ctggcgttct cgaggctcgg ccgccagccg
      781 gtcgccgacc gcgagatcaa tgtcgactcg ctggtccgcg aagtcatcga agaagtcgtg
      841 ggatccgcgc ccgcgccgcg tattgaagtg gagccgttgc cgagcacgcg cggcgatcgc
      901 ggcctattgc gtcaggtatg ggtaaatctc atcgccaacg ccatcaaata cagcagcaaa
      961 gccgcgcagc cgctcattca aatcagcggc cggcaagtcg gcacggaaaa tcattattcg
     1021 gtcagggaca atggcgtcgg cttcaacatg gcctacgtcg gcaagctgtt cggcgtgttc
     1081 caacgactgc accgggccga cgaattcgca gggacgggcg tcggcctcgc catcgtacac
     1141 cgggtagtca cccgccacgg cggcagggta tgggcggacg gcgaagtgga tcgcggcgcg
     1201 gtgttctcgt ttgcgctacc caatggaggc agcaatggat gattttgaag cggtggaaat
     1261 cctgctcgtg gaagacaacg acgccgacgc ggaaatgacc acgcgggcgc tgcgcaaggg
     1321 caatgtcatc aaccatgtgg tgcggctgca cgacggggtc gaggcactcg aattcctgtt
     1381 tcgcgagggc gctttcgccc tgcgccgcgg ccctcagccg cggctcatcc tgctcgacct
     1441 caaaatgccc cggctcggcg gcatcgatgt tctgcggcgc ctgaagtccg acacgggcac
     1501 caagatgata ccggtcgtcg tgctcacttc ctcggcggag gagaaggacg tcgtcgagtc
     1561 ctaccagttg ggcgtgaata gctacctggt aaagccggtt gaattctcgg catttgcgag
     1621 cgtgatcaat cagaccgggc tttattgggc ggtcatgaat cgcatcccga atctctagca
     1681 tgcgccggcc gacaagatac tgtcccaagg cgaactgatc gcgacgcgtt ttcgctgaga
     1741 cccagcgcac caaaccgggg ctttcgattt gaccggctcg aggcgcgcag ctagcctaag
     1801 cagccgtttg ccgggaaaac gattggtaga tgatctggtg catcgcaaca tactcggaaa
     1861 ctcgctcttt tcccggcgtt tcaagcaact gacttcgcgg ctggcgccgt acgctgccgc
     1921 gctgagcgct gcgttgcttc tttggcatgc cgcccgcgac acgccgacct ccgttgcgta
     1981 cgcaccggcc ccgttggtct atccgaccgc gcagcgcgcc gttccggcgc cgagcccgcc
     2041 gccccctgcc gctgccgtgc aatcgagcgc tccggcaatt gcacaacaag cgaatgtcgg
     2101 ggcgaccatt gaagtcgtcg tcggcaggaa cgacaccctt gacgccatat tccgtcgcat
     2161 ggcgctcgac aaggcagacc tcgcggcgat tcgccagttg ccgggcattc gtcagagcct
     2221 cgacttcctg aggcccggcg atgcgataaa actcaaacac agcgacggcg aaatcgaaga
     2281 actcacacgc aaggtgagcg aaactcaaac tctcaaagtc gtgcgcgaag acagcggctt
     2341 cgcggcgcag atgatcaaca atcccgtgga gacgcggatt cgaaccgcga gcgccacgat
     2401 cgactcgtcg ttatttcagg ccgccgaatc cgccgacgtt tccgacatcg tggccctgaa
     2461 actcgccaac atatttgcct gggatatcga cttcgtgctc gacatccgcg agggcgaccg
     2521 gttcaccgcg gtctacgaac aaatctatca agacggaaaa tatctgcgcg acggcgacgt
     2581 gctcgccgcc gagttcgtca acagcggcaa ggtctatcgc gcggtgcgct tcgcggcgga
     2641 cggcagcagc gccagctact actccccgga cggcctgccg ttgcgcaagg cgttcttgcg
     2701 cgccccggtg gaattcacgc gagtgagctc ggtgttcaac ccgcaccgca tgcatcccat
     2761 cctcaacctg attcgcggcc atatgggcac cgactacgcg gcaccgatcg gtacgccggt
     2821 gcatgccgca ggcgacggcc ggatcagctt tgccggaccg cgaggcggtt atggcaatgc
     2881 gttggttctc gcccatagca acagcgtgtc gaccctgtat ggacacttgt cgcgctttgc
     2941 caagcacatt cgcgtcggta cgcatgtgca gcagggcgag gtcatcgcct atgttgggat
     3001 gaccggactc gccaccggcc cgcacctgca ctacgagtac ctcgttgacg gcgtgcataa
     3061 gaatccgcaa accgtgcact tgcccggagc ggagcctttg tccgccgaca tgctcaaaaa
     3121 attccacgct acgaccgccc ccctgctcgc caatctggcg ccgcagccgg cgccctccgg
     3181 cagcgcaccc accgcggcct tcggtccgct cgccgacagc acgccgccgg tggtgccgtt
     3241 ggccggcagt atgtttggcg tatctgccgt actgagcgat gcggacgctc tcgatcgacc
     3301 ggctcttcac gactagtcca ggctgcaatg ggcttctacc tcggcctgat gtccggaacc
     3361 agcatggacg caatcgacgc ggccctggtc gacttcgatg ggtcgccgct gcgcattctc
     3421 gcgaccagcg ccacgcgttt cgatcccgcg ctcaaacagc gcatcgcggc cgttctcgac
     3481 tttccggaca gcgttgctct cgatgagatc ggccagatcg atgtggaatt ggcacgggct
     3541 tttgcggcgg cagcgctcgc gctgctgcgc caagccggcg tcggcagcgg tggcgtcgcg
     3601 gcgatcggca gccatgggca aacattacgt catcgtccgg atctggctgc gccattcacc
     3661 tggcaaatcg gcgatcccaa taccctgagc gaaatgaccg gcatcaccgt ggtggccgat
     3721 tttcgccgcc gcgacgtcgc cgccggcggc caaggtgcgc cgctcctgcc ggtgtttcat
     3781 gaccaggtgt ttcgcagcga acgcgaggat cgcgtcatcg tcaacctggg cggtatcgca
     3841 aacatcacca tcctgcaacg cgatatgccg gtcatcgggt tcgacaccgg gccggccaat
     3901 cgcctgctgg acgcctgggt ctcgttgcag cagggcaagg agcttgatgc cggcggcgag
     3961 tgggccggca ccggtcgatg cgatgacgcg ttgctgcaga agctgctgga cgagccttat
     4021 tttcgtctgg caccgcccaa gagcacgggc cgcgagttgt tcaacctggc gtggctcaac
     4081 cataaactag gctcagcgcc gcggcggccg caggatgtgc aagctacctt gcaacaattc
     4141 acggcggcga cgctcgccgc cgaagtccac agacacgcgc ccggcgcggc actgtacgtg
     4201 tgcggcggcg gtgcgcacaa cgccggcctg ctcgcagcca tagcacggtc ggtggcgccg
     4261 agcacggttg caaccaccac cgccctcggc ctcgatccgg actacgtcga ggccgtcgcg
     4321 ttcgcctggt ttgcacggcg cacgctcgag ggattgacgt cgagtgccgc gagtgtcacc
     4381 ggcgcggcgg ggccgcggat ccttggcggc gtgtatcgat acgcctagtg cgatgaacga
     4441 caccgacctg caagccccgc agctgttcat caatcgcgag ctctcgtttc tggaattcaa
     4501 ccagcgcgtc ctcgagcagg ccaaggacag ccacataccg c
//
LOCUS       DASWZT010000295         5308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_12084, whole genome shotgun sequence.
ACCESSION   DASWZT010000295 DASWZT010000000
VERSION     DASWZT010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12084"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VGE92_08045"
     CDS             <1..230
                     /locus_tag="VGE92_08045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1313813.1"
                     /translation="VGVIDNGVGIAPENMHRLFTHGFTTRASGHGFGLHSGALAAQEL
                     GGTLSVKSDGPGRGATFTLELPYDAADAKHV"
     gene            223..2175
                     /locus_tag="VGE92_08050"
     CDS             223..2175
                     /locus_tag="VGE92_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY1313814.1"
                     /translation="MSDATPPGNRRILIIDDNAAIHLDFRKVLGEQVEHTAQAALDVL
                     EANLFGDAVTPAARPNFDIDSAHQGEEGVAMAHQAKIDGRPYAMAFVDMRMPPGWDGL
                     KTIERLWATDPDVQVVICSAHTDYDWIEVVERLGHSDKLLVLRKPAEPIEVLQCATAL
                     CRKWQNDKLVRENVQRLEEVITVRTRGLEAANQQLRHVALHDALTGLPNRVLLDDRLQ
                     QAIAHADRDLRAFAVLICDLDRFKLINDSLGHRAGDELLQEVARRLNTVVRAADTVAR
                     FGGDEFVLIGTSIVDADDAAGFAARVMEVLQAPVRIADIDIHTSASIGIAMYPEDGIS
                     TQALLAHADAAMYCAKQRGRSNSQRYVTGMNAGTEDRVQLESDLHNAVALDQFRLYYQ
                     PKVDIRTGEVRSAEALIRWVHPTRGIVSPADFIPLAEDCGMIGAIGDWVIREACRQTR
                     AWQVAGVPLLRISVNLSASQFGDSGLVDSIRRELEGAALEARYLEVELTESVVMSDPE
                     KSIAILEQLSSMGVLISVDDFGTGYSSMSYLRRFPIDKLKIDRVFIDEIVSRPEDASI
                     VRAIVSLAHSLHLKVVAEGVETPAQLYFLKTAGCDEYQGYHFSRPLPAAEFERLVRES
                     HADDVLTEDEAVRTHSKLAAYRRDSR"
     gene            complement(2172..3962)
                     /locus_tag="VGE92_08055"
     CDS             complement(2172..3962)
                     /locus_tag="VGE92_08055"
                     /inference="COORDINATES: protein motif:HMM:NF042569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase kinase/phosphatase AceK
                     regulatory subunit"
                     /protein_id="HEY1313815.1"
                     /translation="MPTDTELAVAEGYSRLETCSDQAARIPAAAQLILTIYVEFYRQL
                     CEYPNLAKRAFESMDPHASIRISQERLGLYSRYIAEHGPRILAAFPALAGDASVWDAL
                     DRLFVAMIVDRYDADIAFSFLHSIRRNIGRGLWRPVAYSFPPPSKLRADSMAAVHRRL
                     RVHGRIDTELMCTALRMPGFTVPFIDLQGDAERILKRVGPLLCSPYATPVLALDVVEA
                     GFFRDRSAFLVGRWVLADEGLVPFVVAFKNSAQGIYVDAVLDRVSDIHDLFSSALANF
                     HVTTRLYYQTCVFLFSLMPRRPLGHQYSTIGFNHVGKVAILNEISEQVRRGHRLQRSP
                     GVPGTVALGFTFDACAYHLKVIRDQPTSSYKWGAYPGVAAVSEKYRVVHEINRAGSML
                     DNVMYFNLRLDRDMFDPKLLDEICVQAAGSVQVDGDNVYLRTLIVQLKIVPLPVFLET
                     ADDHAIRAVMVRLGQCIRNNAATNIFNKDLDSRNYGVGRYGRVFLFDYDAVEKFTDVK
                     IRTNTDREPGEEAVPDWFYEDGVIFLPEELEHGLQLKSSYARRCFREENFDLLQVDYW
                     QDVQQKLQRGEVPALQMYPDSCKLNPDAAR"
     gene            complement(3968..>5308)
                     /locus_tag="VGE92_08060"
     CDS             complement(3968..>5308)
                     /locus_tag="VGE92_08060"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEY1313816.1"
                     /translation="SYSEETLSELAIVHAAADAKRRYGAGCITTYIVSKCESVSDLLE
                     VNILLKEAGMYSARGKPHAAIMAVPLFETIGDLEKASEVMSAWFALPEVAAVCAGHGF
                     QEVMVGYSDSNKDGGYLTSVWSLHQATSSLAEVFEKFATPMQIFHGRGGAVGRGGGSS
                     FAAIRAQPYGTVRGRIRITEQGEIIAAKYGTRDSAAANLESISAATLLASLENASLSS
                     ANRLRFGAAMDELSAHAFRAYRSLVYETAGFATFFRQMTPLVEISALKIGSRPASRSN
                     SQRIEDLRAIPWVFSWAQARVMLPGWYGVGEALRSADRGLLREMLEAWPFFRATVDNL
                     EMVLSKSDMGIAARYASLVEDRNLADHLFGRIRDAWVATCESLFAVTEQSRLLEKHPA
                     LDDSIRLRLPYIEPLNLLQVELLKRHRAGEKDPRVREGIQLSINAIATALRNSG"
BASE COUNT         1000 a         1712 c         1655 g          941 t
ORIGIN      
        1 gcgttggcgt catcgacaac ggcgtcggca tcgcgccgga gaatatgcac cgcctcttca
       61 cccatggttt taccacccgc gcgtccggac acggcttcgg cctgcacagc ggcgcactgg
      121 cggcgcagga actcggcggc acgctcagcg tcaagagcga cggcccggga cgcggcgcca
      181 ccttcacgct cgagctgccc tacgacgccg cggatgccaa gcatgtctga cgctacgcca
      241 cccggcaatc ggcgcatatt gatcatcgac gataacgccg ccatccacct cgattttcgc
      301 aaggtgttgg gcgaacaagt cgagcacacc gcgcaagccg cgctcgatgt gctcgaggcg
      361 aatcttttcg gcgatgcggt aacccccgcc gcgcgcccga atttcgacat cgattcggcg
      421 caccaaggcg aggaaggcgt cgccatggcg catcaagcca agatcgatgg gcggccgtac
      481 gcgatggcct tcgtcgacat gcgcatgccg ccgggctggg acgggctcaa gaccatcgag
      541 cgcctgtggg caaccgatcc cgacgtgcaa gtggtcatct gttcggcgca caccgactac
      601 gactggatcg aagtcgtcga gaggctcggc cactccgaca agctgctggt gctgcgcaaa
      661 cccgccgaac cgatcgaggt cttgcagtgc gccaccgcgc tctgccggaa gtggcaaaac
      721 gacaagctgg ttcgagaaaa cgtgcagcgc ctcgaagagg tgatcaccgt gcgcacccgc
      781 ggcctggagg cggcgaatca gcagttgcgg cacgtggcgc tgcacgatgc gctgaccggg
      841 ttgccgaatc gggtgctgct cgacgatcga ctgcaacagg ccatcgcgca tgccgaccgc
      901 gacctgcgcg cgttcgccgt gctgatttgc gatctcgacc gattcaagct catcaacgac
      961 tccctgggtc accgtgccgg cgatgaactg ctgcaggagg ttgcgcgccg gctcaacacg
     1021 gtggtacgcg cggccgatac ggtggcgcgg ttcggcggcg atgagttcgt gctgatcggt
     1081 acttcgatcg tcgatgccga cgatgccgcc ggtttcgcgg ctcgtgtgat ggaagtgctg
     1141 caggcgccgg tgcggatcgc cgacatcgat attcacacgt ccgcgagcat cggcatcgcc
     1201 atgtatccgg aagacggcat ttccacgcag gcgctgctgg cgcatgccga cgccgccatg
     1261 tactgcgcca agcaacgcgg gcgcagcaat tcgcagcgct acgtcactgg aatgaacgcc
     1321 ggcacggaag atcgcgtgca gctcgagagc gacttgcaca atgccgtcgc cctcgatcaa
     1381 ttccgactgt attatcagcc aaaagtggac atccgaaccg gcgaggtgcg cagcgccgag
     1441 gccttgatcc gctgggtgca cccaacccgc ggcatcgtct cccccgcgga tttcatcccg
     1501 ctcgccgaag actgcggcat gatcggcgcc atcggcgact gggtgatccg cgaagcgtgc
     1561 cgccagacgc gcgcctggca ggtcgccggc gtgccgctgc tgcgcatctc ggtgaatcta
     1621 tccgcctcgc aattcggcga cagcggcctg gtcgacagca ttcgccgtga gctcgagggc
     1681 gcggcgctcg aggctcgcta cctcgaggtc gaactcacgg aaagcgtggt gatgagcgat
     1741 ccggagaaat cgatcgccat tctcgaacaa ttgagttcca tgggagtgct catctcggtt
     1801 gacgatttcg ggaccggcta ctcgagcatg agctacttgc gccgcttccc gatcgacaaa
     1861 ctcaagatcg accgcgtgtt catcgacgag atcgtcagcc gtccggagga tgcatcgatc
     1921 gtgcgcgcca tcgtctcgct ggcgcacagc ctgcacctga aggtcgtcgc cgaaggggtc
     1981 gagacgccgg cgcagctgta cttcttgaaa accgccggct gcgacgaata tcagggctat
     2041 catttcagcc gcccgctgcc ggccgcggag ttcgagcgcc tggttcgcga atcgcatgcc
     2101 gacgatgtac tgaccgagga cgaggccgtt cgcacgcaca gcaagctcgc cgcgtaccgc
     2161 cgagatagcc gctagcgcgc ggcgtccggg ttcagtttgc agctgtcggg gtacatctgc
     2221 agcgccggca cctcgccgcg ctgcaatttc tgttgcacgt cctgccagta gtcgacttgc
     2281 aatagatcga aattctcctc gcggaagcag cgtcgcgcgt aggaactctt gagctgcagg
     2341 ccgtgttcca gttcttcggg caggaaaatc acgccgtcct cgtaaaacca atccggcacc
     2401 gcctcttcgc cgggctcacg atccgtgttg gtgcggatct tgacgtcggt gaatttctcc
     2461 accgcatcgt agtcgaagag aaacacgcgg ccgtagcggc cgacaccgta gtttctcgaa
     2521 tccagatcct tgttgaaaat attggtggcg gcgttgttgc gaatgcattg cccgagccgc
     2581 accatgacgg cgcggatcgc gtggtcgtcg gcggtttcca aaaaaaccgg cagcggcacg
     2641 attttcagct gcacgatcag cgtgcgcaga tacacgttgt cgccatccac ctgcacgctg
     2701 ccggcggctt gcacgcagat ttcatccaac agcttaggat cgaacatgtc gcgatccagc
     2761 cgcaaattga agtacatcac gttgtcgagc atgctgccgg cgcgattgat ctcatgcacc
     2821 acgcggtact tctccgagac ggccgcgact ccggggtacg cgccccactt gtaggaactg
     2881 gtcggttgat cgcgaatgac tttcagatgg taggcgcagg catcgaaggt gaatccgagc
     2941 gcaaccgtgc cgggcacgcc gggcgagcgt tgcagcctgt ggccgcggcg cacctgctcg
     3001 ctgatctcgt tcaatatggc gaccttgccg acatgattga agccgatggt cgaatactga
     3061 tgaccgagcg gacggcgcgg catcaagctg aacaggaaca cgcacgtctg gtagtacagg
     3121 cgggtggtca cgtgaaagtt cgcgagcgcc gaactgaaca ggtcatggat gtccgagacg
     3181 cggtcgagta cggcgtcgac gtagattccc tgcgcgctgt tcttgaaggc gaccacgaac
     3241 ggcacgagac cctcgtccgc cagcacccag cggccgacca agaacgcgct gcgatcgcgg
     3301 aaaaatcccg cctcgaccac atcgagtgcg agcaccggcg ttgcatacgg gctgcacagc
     3361 agcggtccga cccgctttag aattcgctcg gcgtcgcctt gcaagtctat gaacggcacc
     3421 gtgaacccgg gcatccgcaa cgccgtgcac atgagctcgg tgtcgatgcg cccatgcacg
     3481 cgcagccggc gatgcaccgc cgccatcgag tccgcgcgca gcttgctcgg cggcggaaac
     3541 gaataggcca ccggcctcca cagtccgcgg ccgatgttgc gccgaatcga gtgcaggaaa
     3601 ctgaatgcga tgtcggcgtc gtatcgatcg acgatcatcg ccacgaacag ccggtccaag
     3661 gcgtcccaca cgctcgcatc gcccgcgagt gcggggaacg ccgcgagaat cctcggcccg
     3721 tgctccgcga tgtagcggct gtacaaaccc aggcgttcct gactgatgcg gatcgaggcg
     3781 tgcggatcca tgctttcgaa ggcacgcttt gccaggttgg ggtattcgca cagctggcgg
     3841 tagaattcca cgtagatggt cagtatcagc tgcgcggccg cgggtatgcg cgcggcttga
     3901 tcgctgcagg tttcgaggcg ggaatagcct tccgcgacgg cgagttcggt gtcggtcggc
     3961 atcgcggcta accgctgttg cgcagggccg tggcgatcgc gttgatcgac agctggatgc
     4021 cttcgcgcac ccgcggatcc ttctcgccgg cgcgatggcg cttcagcagc tcgacctgca
     4081 gcagattcaa gggttcgata tacggcaggc gcagccggat cgaatcgtcc aatgccggat
     4141 gcttttccag caagcgcgat tgctcggtga cggcgaacag gctttcgcaa gttgcaaccc
     4201 aggcgtcgcg aatcctgccg aacaagtggt cggcgaggtt gcgatcctcg accaggctcg
     4261 catagcgcgc cgcgatgccc atgtcggatt tcgacagcac catctcgaga ttatccacgg
     4321 tggcgcgaaa aaacggccac gcctcgagca tctcgcgcaa caatcctcta tccgcggaac
     4381 gcaatgcctc cccgaccccg taccagcccg gcagcatgac gcgcgcctga gcccagctga
     4441 acacccacgg gatggcgcgc agatcctcga tacgctgcga attcgagcgc gacgccggcc
     4501 gcgagccgat cttgagcgcg gagatttcca cgagcggcgt catctgccga aaaaacgtcg
     4561 cgaaccctgc ggtttcatag accaaggaac ggtatgcccg gaatgcatgc gccgacaact
     4621 cgtccatggc cgcgccgaat cgcagccggt ttgcgctcga cagcgaggca ttttccagcg
     4681 acgccagcag cgtcgccgcg ctgatcgact cgagattggc cgccgcgctg tcgcgcgtgc
     4741 cgtacttggc cgcgatgatt tccccttgct cggtaatacg gatgcggccg cgcaccgtac
     4801 cgtacggctg ggcgcgtatc gctgcgaagg aggaacctcc gccgcggccg acggcgccgc
     4861 cgcggccgtg aaagatctgc atcggggtcg cgaatttctc gaacacttcc gcgagcgagc
     4921 tggttgcttg atgcaagctc cacacggagg tgagatatcc gccatccttg ttcgagtccg
     4981 aatatccgac catcacctcc tgaaacccgt gaccggcgca taccgccgcc acctcgggca
     5041 gagcgaacca tgcgctcatg acctcgctgg ccttttcgag gtcgccgatg gtttcgaaca
     5101 gcggcaccgc catgatggcc gcgtgcggct tgccgcgcgc gctgtacatg ccggcttcct
     5161 tcagcaagat gttcacctcg agcagatccg acacgctctc gcatttggag acgatgtagg
     5221 tcgtgatgca gccggcgcca tagcgccgct tggcatcggc ggccgcatgc acgatggcga
     5281 gctcggatag ggtctcctcc gagtagct
//
LOCUS       DASWZT010000296         2555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_45944, whole genome shotgun sequence.
ACCESSION   DASWZT010000296 DASWZT010000000
VERSION     DASWZT010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45944"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1411
                     /locus_tag="VGE92_08065"
     CDS             <1..1411
                     /locus_tag="VGE92_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY1313817.1"
                     /translation="LLSALLSLLLTLAGSQGWAQTQPRAFLGIGLADIPGGGGAVVGV
                     IAPGSPAERAGLQIRDLVTAVNGSAVARAAEVTDAVLAMRPGDTAHLAVLRGRGAAAQ
                     QLSIDVTLGTSPGASAPAAPNSADSAASEPKPAAALPGRNHAPTAAAASPRPLAVEGY
                     TNFSDPLEQAFTIQVPVGWSSVGGLARRSALQINPFLRSVSPDKMTYLMIGEPGLPSY
                     VPPTPLRNTLGYREGKLFDSGLGGLSMVLHFMRGTEFAQAYGQTVLSGLCTAVRYQQS
                     AERQDMAQSADRLVPTVIPSLSSGGEARFSCTHNGQPMEARIEVVTRTTRDNVMWNVI
                     FLKGWLAPRTDAERAEEILTHVGASLAFDPAWLQKQSNLDQQAANSINRSMQEFFRRQ
                     QAVVQKLNAVDTSFSSMDEIVSGYSTYHDAATGQDYKLDNTNPYKWIDNSTSRIFSTP
                     NNIPPPWGGALAPLAHLAQ"
     gene            1660..>2555
                     /locus_tag="VGE92_08070"
     CDS             1660..>2555
                     /locus_tag="VGE92_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313818.1"
                     /translation="MMRIKPAFQTSMFEKVLRAFGTGDLPYTYVQSELKRLLETGESP
                     HKLLEVMQRRELIEPLPEKAHEEVLRTLNEAIERAAAPNPPQNPASIPGAVAANGESA
                     SAPPGSAEAESAASPADGAPSLEELYASLKSDFESLARSFEDAQDRGTAATSRAVSLG
                     ADLAAARAALAAEQNKSREMGKALAEKSTLNDAARSRSDAALRQAEQFQGEVRQLRDM
                     LGARDKTLAQARHQLAEREAELATLKQEHARTITELEARAKSAEAELRTMRTQADTVA
                     SSYVELLQNREWRQGFDQNRSR"
BASE COUNT          491 a          817 c          830 g          417 t
ORIGIN      
        1 gttgttgtcg gcgcttctgt cgcttctgtt gacgctggca ggatcccaag gttgggcgca
       61 gacgcagcca cgtgcctttc tcggaatagg tcttgccgat attcccggcg gcggcggtgc
      121 cgtggtcggc gtgatcgcgc cgggcagtcc ggccgagcgg gcggggctgc agattcgcga
      181 tctcgtgacg gccgtgaatg ggtccgcggt ggcgcgtgcg gccgaggtga cggatgcggt
      241 gctcgccatg agacccggcg acacggcaca cctcgcggtg ttgcgcggcc gcggcgccgc
      301 ggcgcagcag ctgtcgatcg atgtcacgct cggcacctcg ccgggcgcat cggcgccggc
      361 ggcgcccaac tcggccgact ccgcggcgag cgaaccgaaa ccggccgcgg cgctgccggg
      421 ccgcaatcac gcgccgaccg ccgccgccgc gagcccgcgg ccactcgcag tcgagggcta
      481 tacgaacttt tccgatccgc tggaacaggc cttcaccatc caagtaccgg ttggttggag
      541 ttcggtgggc ggcctcgcgc gccgcagcgc cttgcagatc aatccgttcc tgcgctccgt
      601 gtcgcccgac aagatgactt atctcatgat cggggagccg ggattgccgt cctacgtgcc
      661 gcctacgccg ctgcgcaaca ccttgggtta ccgcgagggc aagctgtttg attcgggtct
      721 cggaggtctg tcgatggtcc tgcacttcat gcgcggcacg gaattcgcgc aggcgtacgg
      781 acagacggtg ctgtcgggtc tgtgcacggc agttcgctat cagcagtcgg cggagcggca
      841 ggacatggca cagagcgccg accggctggt gcccaccgtc attccttcgc tatcgagcgg
      901 cggcgaggcg cggttctcct gtacgcataa tggccaaccg atggaggcgc gaatcgaggt
      961 cgtcacccgc accacgcgcg acaacgtcat gtggaacgtg atattcctca agggctggct
     1021 cgccccgcgc accgacgcgg agcgcgcgga agagattctg acgcatgtag gagcgagctt
     1081 ggcgttcgat ccggcgtggc tccagaagca gagcaatctc gaccaacagg cggcgaattc
     1141 gatcaaccgc agcatgcagg agttttttcg ccgccagcag gcggtcgtgc agaagctaaa
     1201 tgcggtggat acgagttttt cgtcgatgga cgaaatcgtc tccggttaca gcacctatca
     1261 cgatgctgcg accggccagg actacaagct cgacaacacc aacccctaca aatggatcga
     1321 caacagcacc agcagaatat tctccacgcc caacaacatc ccgccgccat ggggtggagc
     1381 gctggcgccg ctcgcacacc tcgcgcagta gcggcccaaa ccccttgttt ttctgcgcca
     1441 cgcgcacgcg cgcattatgt cgaacgcctt atgccaaact cgcaaatcat tgtttagatg
     1501 agcgcaacaa tgcaaaatga gacctgcgac acacgctcac ggacgccatc cgcgggttga
     1561 ggtcgaagag cgaatttccc gaaccctcgg aatgcattgc taacgtcgag ctgcaaaatc
     1621 ttgatcgagt gcggacgctg acgagcgtca agaaagccca tgatgcgtat taagcctgca
     1681 ttccagacgt cgatgttcga gaaggtgctg cgagccttcg gaaccggcga cctgccgtat
     1741 acctatgttc agtcagaact caagcggctg ctcgagacgg gtgaatcgcc gcacaagctg
     1801 ctggaagtca tgcagcggcg cgagctgatc gagcccttgc cggagaaggc gcacgaggaa
     1861 gtgctgcgca ctctcaatga agcaatcgaa cgcgccgccg cgcccaatcc gccgcaaaat
     1921 ccggcatcga ttccgggcgc ggttgccgcc aacggcgaga gcgcatcggc gccgccgggc
     1981 agtgcggagg ccgagtccgc cgcatcgccg gccgacggcg caccctcgtt ggaagagctg
     2041 tatgcatcgc tcaagtccga cttcgaatca ttggcgcgca gtttcgagga tgctcaggat
     2101 cgcggcaccg cggccacatc gcgcgcagtc tcgctgggcg cggatctcgc ggctgcccgc
     2161 gccgcgctcg ccgccgagca aaacaagagc cgcgaaatgg gcaaggcgct ggccgagaag
     2221 tccacgttga acgatgcggc gcgctcccgc agcgacgccg cactgcggca ggcggaacaa
     2281 ttccaaggtg aggtacgcca gctgcgcgac atgctcggcg cgcgcgacaa gaccctggcg
     2341 caagcgcggc atcagctcgc ggagcgcgag gccgaattgg ccacgctcaa gcaggagcat
     2401 gcgcgaacga tcaccgaact cgaggcgcgc gccaagagcg cggaggcaga actgcgcacg
     2461 atgcgcacgc aggcggatac ggttgcgagt tcctacgtcg agctgctgca aaatcgcgag
     2521 tggcgtcagg gattcgatca gaatcgttcg cgtga
//
LOCUS       DASWZT010000297         2236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_36136, whole genome shotgun sequence.
ACCESSION   DASWZT010000297 DASWZT010000000
VERSION     DASWZT010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36136"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2236
                     /locus_tag="VGE92_08075"
     CDS             <1..>2236
                     /locus_tag="VGE92_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313819.1"
                     /translation="IAAGQSAEWVPPAWRRSLLNLTTSLEKPHFVTYVLRVYDAAGHF
                     DETRPRRLDLTDTAPLQTLDADALARSTERAAYGENTLVLHNIASRGGAITVSGSHVP
                     PGDTVLVQNIPIPVDEDRHFVARQILPDGPQQVSVKILNARGEGLDFTRNIVVASDDS
                     FFVGLADFTAGARSSSGPIDLVSGATAEQTRRDYVNGQLAFYYKGLVKGEWLLTAAAD
                     TQDQPVRDLFSNFARKDPQDLLRRIDPDRYYPVYGDDSTTVQDAPTAGKFYVRLEKGD
                     TSVLWGDFQTHLNGTDFMQFARTLYGLDIRYRSPQTTTLGEKTRAVDAFWADPGTLES
                     RQEFRGTGGSLYYLQNQDISVGSEQVWMQVRDRDSGIVLSVTPMVPAQDYDINYLQGR
                     ILLHNPLPATANSATVVHSGGLDGDPVYLVVTYEYVPDFSSPSTLALGGHASEWFGDH
                     LQLGISGYHQSDPGQDQDLRGLDGTLRYKPGTYIKSEFAHSDGTGTTSLTSITGGLSF
                     NSLTTAGGPGNAERIEAAVDLAEVSDLKGRANVYYQDRDANFSGPGQLAPGVTPGVGV
                     RQEGAAVNLPVNSTTQLAGKFDSTDSTQQTLRSGELGVEHKLDEHWRITLGTRIDDRE
                     NAVPNASPILSQNGRRTDVAVTVGYQPAAGVVAPVAGAPAAAAPALIGATRAVGTTTV
                     PGVGTGTASVAGASSAAGGVDAGSATGASSVAPSAPSAADSGASATPVSAARPPWDVY
                     GFVQE"
BASE COUNT          415 a          709 c          731 g          381 t
ORIGIN      
        1 gatcgccgcc ggccagtcgg cggaatgggt gccgccggcg tggcgtcggt cgctcttgaa
       61 tctgacgacg tcgttggaga agccgcactt cgtgacctac gtattgcgcg tctacgacgc
      121 ggcgggacac ttcgacgaaa cccggccgcg gcgtctggac ttgaccgata ccgcgccgct
      181 gcaaacgctg gacgcggatg cgctggcacg cagtaccgaa cgggccgcat acggcgagaa
      241 cacgctggtg ctgcacaata ttgcgtcgcg cggcggcgcc atcaccgtga gcggcagtca
      301 tgtgccgccg ggcgatacgg tgctggtgca aaatattccg ataccggtgg atgaggatcg
      361 gcatttcgtt gcacgccaga tcctgcccga tggtccgcaa caagtcagcg tgaaaatact
      421 caacgcgcgc ggcgaaggtc tcgatttcac gcgcaacatc gtggttgcga gcgacgactc
      481 gttcttcgtc ggtttggcgg atttcaccgc gggagcacgc agcagcagcg ggccgatcga
      541 cctcgtgagc ggcgcaacgg cggagcagac gcgccgcgat tacgtgaatg gtcagttggc
      601 cttctattac aagggcctgg tgaaaggcga gtggctgctg accgccgcgg ccgatacgca
      661 ggatcaaccg gtgcgcgatc tgttcagcaa tttcgcccgc aaggatccgc aggatctact
      721 gcggcgcatc gatccggacc gctattatcc ggtgtacggc gatgacagca ccacggtgca
      781 ggatgcaccc accgccggca agttctacgt tcgcttggag aagggcgaca cgtcggtgtt
      841 gtggggagat ttccaaaccc atctgaacgg caccgacttc atgcagttcg cgcgcacttt
      901 gtacggcctg gatattcgct accgatcgcc gcagacgacg acgctcggcg agaagacgcg
      961 cgccgtggat gcattctggg ccgatcccgg caccctcgag tcccgccagg agtttcgcgg
     1021 caccggcggc tcgttgtact acctgcagaa tcaagacatc tcggtcggct cggagcaagt
     1081 atggatgcaa gtgcgagacc gggattccgg aatcgtgctg tcggtgacgc cgatggttcc
     1141 ggcgcaggac tacgatatca actacctgca aggacgcatt cttctgcaca acccgctgcc
     1201 cgcgaccgcc aactccgcga ccgtcgtaca cagcggcggt ctcgacggcg atccggtata
     1261 tctggtcgtg acctacgagt atgtcccaga tttctcgagc ccgagcacgc tggcattggg
     1321 cggtcatgcc agtgagtggt tcggcgatca tttgcaactg ggcatatccg gctaccacca
     1381 gagcgatccg ggtcaggatc aggacctgcg cggcctcgat ggcacgcttc gctacaaacc
     1441 cggcacctat ataaaatcgg agttcgccca ttccgatggc accggcacca cgtcgctgac
     1501 ttcgatcacc ggcggcctgt ccttcaattc gctgaccacc gcgggcggtc cgggcaacgc
     1561 ggaacgcatc gaagcggcgg tcgatctggc ggaagtcagc gacctgaagg gtcgtgccaa
     1621 tgtgtactac caagaccgcg acgccaattt ctcagggccc ggccagctgg cacccggcgt
     1681 gacaccgggc gtcggcgtgc ggcaggaagg tgctgcggtc aatcttccgg tcaactcgac
     1741 gacgcaactg gccgggaaat tcgacagcac cgactcgaca cagcaaacgt tgcgcagcgg
     1801 cgagctcggg gtcgagcaca agctcgacga acactggcgg atcacactcg gaacccggat
     1861 cgatgatcgc gaaaacgccg tgcccaatgc cagccccatc ctgtcgcaga atggacggcg
     1921 caccgatgtg gccgtcacgg tcggctatca gcccgcggca ggtgttgttg cgccggtggc
     1981 cggagcgccc gcggcagccg cgcccgcact gatcggcgct acacgtgccg tcggcaccac
     2041 gacggtgccc ggcgtgggca cgggcactgc gagcgtcgcc ggcgcaagtt ccgctgccgg
     2101 tggtgtcgat gccggcagcg caaccggggc gagttcggtc gcgccgtcgg cgccgagcgc
     2161 ggcagattct ggcgcgagcg cgacgccggt gagtgcggct cgtccgccct gggacgtcta
     2221 cggcttcgtg caagag
//
LOCUS       DASWZT010000298         4237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_74879, whole genome shotgun sequence.
ACCESSION   DASWZT010000298 DASWZT010000000
VERSION     DASWZT010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74879"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VGE92_08080"
     CDS             <1..749
                     /locus_tag="VGE92_08080"
                     /inference="COORDINATES: protein motif:HMM:NF027552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4886 domain-containing protein"
                     /protein_id="HEY1313820.1"
                     /translation="PALFKSFSAQAGLDYDVYLETESGVSIDWHLEHKLSVIEQRAWD
                     VAVLQGYSTLDLKKPGDPTALIRAVQRVAAVLRARNPAIDLRLMATWSRADQTFEPTG
                     AWYGKPIEAMARDVRAGYNRVAQATPGVKSVVPVGEAWNRAMQTGVADANPYDGIDAG
                     KVDLWTYDNYHASTYGYYLEALVVFGSLTARDPRSLGEAECSGYELGLSTAQVRALQR
                     VAFDQLAAEGAVRAAAPMPGGTKSASRCAR"
     gene            753..1055
                     /locus_tag="VGE92_08085"
     CDS             753..1055
                     /locus_tag="VGE92_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313821.1"
                     /translation="MGRTRHNPFANSKVDHYVTVWDLHWGVIDCQRLGSGGVDLRGAL
                     GAALRRLELEGWTAQGSGDFGFVFIMRGTERRLVTLSSRDPHNATAQSFSPFGARL"
     gene            complement(1078..1569)
                     /locus_tag="VGE92_08090"
     CDS             complement(1078..1569)
                     /locus_tag="VGE92_08090"
                     /inference="COORDINATES: protein motif:HMM:NF023010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HEY1313822.1"
                     /translation="MPGLKDDIPDSLKSVRTRPLFVMRLDVKPLQLVGATPAGFRRVG
                     VVPGGVFEGDRLSGMVLDGASDWQTLRSDGATALDVRLILKTNDGALISMTYRGLRHG
                     PAEVMARLDKGEAVDAASYYFRMTPGFETAAADYDWINRIVAVGVGERRADGPIYSVF
                     EVL"
     gene            1703..3025
                     /locus_tag="VGE92_08095"
     CDS             1703..3025
                     /locus_tag="VGE92_08095"
                     /inference="COORDINATES: protein motif:HMM:NF024686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY1313823.1"
                     /translation="MAWRFYLGACLSAAATLANAQPYGLEARVAPPAYLHMPPLASGK
                     IPRLLSQTGAFSSMTDLAPAKGLIPYDLVVAFWSDGADKTRWASIPAGKIKYSPTDDW
                     YFPKGTVFVKTFELPIDAAHPAVKRRLETRVLVLDSAGGVYGVDYKWRADLSDADLLE
                     NSQTESISIKTAGGDVRQTWYYPSRADCRTCHNAHTSGVLGVKARQMNHPYTYPGGVT
                     DNEIRAWNHLGLFAPGVTDTATLPTLAAADDAARSLQDRARSYFDANCSHCHRPGVTV
                     AYFDARYNTPLEQQELIDGPVLIDQGIDRPRVISPHDIWRSIAFMRVNTVEDIRMPPL
                     ARETIDQKGVRLLREWITSLPGRSVLDPPAISPQGGTFGGPTAVTLSELEPGADIRYT
                     LDGSVPSLSDNRYAGPIKLTGPVVLRARAFKEGFTRSITVQQVFIIGP"
     gene            complement(3033..4100)
                     /locus_tag="VGE92_08100"
     CDS             complement(3033..4100)
                     /locus_tag="VGE92_08100"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEY1313824.1"
                     /translation="MSMRVQLLGTALLVVSPLGAQAAQNYQVYVSNEKSGDVTVINGK
                     DNQVLATIPLGKRPRGIHASPDGKTVYVALSGTPIAAPPKLDAKGNPVFERGKDDDDD
                     SVKSDKSADAIGVVDVAQRKLTGKISGGSDPEEFSLSKDGTKLYVSNEDVKAASVINI
                     ASGKVEHIIAVGQEPEGVATAPDGARFYITCEAGGDVYAIETAGYTMVGHFKVNVRPR
                     SMDFLPGGAIGFIPSESTGELNIIDTLNLKVSKVIALPPGSRPMSVKVAADGQKVYVS
                     TGRGGTVAVLDSHTYELLSTIQVGTRPWGMALSPDGKFLYTANGPSNDVSVVDVAANK
                     EIARVKAGTSPWGVAIVANTP"
BASE COUNT          781 a         1369 c         1283 g          804 t
ORIGIN      
        1 tgccggcctt gttcaaatca ttcagcgcgc aagcggggct cgattatgac gtgtacttgg
       61 aaaccgagtc cggcgtcagc atcgactggc acctcgagca caaactgagc gtcatcgagc
      121 aacgcgcatg ggacgtggcg gtcctgcaag gctacagcac gctcgatctg aaaaagcccg
      181 gcgacccgac cgctctgatc cgcgccgtgc agcgggtggc agcggtgctg cgcgccagga
      241 atccggctat cgacctgcgg ctgatggcaa cctggtcgcg cgccgaccaa acattcgagc
      301 ccacgggggc ctggtacggc aaaccgatcg aggccatggc acgcgatgtg cgcgcgggct
      361 acaaccgcgt cgcccaagcg acccccggcg tcaagtcggt ggtacccgtt ggcgaggcgt
      421 ggaatcgcgc catgcaaacg ggcgtcgccg acgccaatcc ctacgacggc atcgacgccg
      481 gcaaggtcga tctgtggact tatgacaact accatgcgag cacctacggc tattacttgg
      541 aagccttggt tgtgtttggc agccttaccg cgcgtgatcc tcgctccttg ggcgaggccg
      601 agtgttcggg ctatgaattg gggctgtcca cggcgcaggt ccgagccctg cagcgggtcg
      661 cattcgatca attggcggcg gaaggtgcgg tgcgcgccgc ggcgccgatg cccggcggca
      721 ccaagagcgc ctcgcgctgt gcgcgctgaa cgatgggccg cacccgccac aatccgtttg
      781 ccaattcaaa ggtggatcac tacgtcaccg tctgggacct gcactgggga gtcatcgact
      841 gccagcggct gggcagcggc ggcgtcgatc ttcgcggcgc gctcggcgcg gcgctgcgcc
      901 gactcgaact cgagggctgg acggcacaag gcagcggcga tttcggtttt gtattcatca
      961 tgcgcgggac cgagcgacgg ctggtgactc tgtcctcgcg ggatccgcat aacgcgacgg
     1021 cgcaatcctt ctcccccttc ggggcacggc tctaaccggc ttgcggcggc aaggccttca
     1081 caggacttcg aagacgctgt aaataggccc gtcggcgcgg cgctcaccga ctccgaccgc
     1141 cacgattcga ttgatccaat cgtagtcggc cgccgcagtt tcgaaccctg gagtcatgcg
     1201 aaagtaataa ctcgccgcat ccacggcctc gcccttgtcg agtcttgcca tcacctcggc
     1261 cggaccatga cgcaagccgc gatacgtcat cgaaatcagc gcgccatcgt tggtcttcaa
     1321 gatcagccgc acatcgagtg ccgtcgcacc gtcgctgcgc agtgtctgcc agtcgctcgc
     1381 tccatccaac accatgccgg atagcctgtc gccctcgaac acgccgccgg gaacgactcc
     1441 cacgcggcga aagcccgcgg gtgtcgcgcc gaccagctgc aagggtttga catccaggcg
     1501 catcacgaac aagggtcgtg tgcgcacact tttcagggaa tctggaatat cgtccttcaa
     1561 tccaggcatg ttcaggctcc attggcccat aattacgcgg ctgcgcccgg acaaagtata
     1621 tattgcgcct agtgaagcgt gactgaagga tgaggcgatc aatcaccgga accaacgacc
     1681 gcgaactggg tcaacatcga ctgtggcgtg gagattttat ctaggcgcat gcttaagcgc
     1741 cgctgcaacg ctcgccaacg cacagcccta cggcctcgag gcgcgggtcg caccgccggc
     1801 gtacttgcac atgccgccgc tcgcgagcgg caagattccg cgattgctgt cgcagaccgg
     1861 cgccttcagt tcgatgaccg atcttgcccc ggccaaaggg ctgattccct atgatctggt
     1921 ggtggcgttt tggtccgatg gggccgacaa gacgcgctgg gcgtccatac cggcgggcaa
     1981 gatcaaatac tcgccgacgg acgattggta ctttccgaag ggcaccgtct tcgtcaagac
     2041 tttcgaattg ccgatcgacg cagcccatcc ggcggtgaaa cgccggctgg aaacgcgcgt
     2101 tttggtgttg gatagcgccg gtggcgtgta cggcgtcgat tacaaatggc gggcggatct
     2161 aagcgatgcc gatttgctcg agaacagcca aaccgaatcg atatcgatca agacggcggg
     2221 cggcgatgtg cgccagacct ggtactaccc gagccgcgcg gactgccgga cttgtcacaa
     2281 tgcccacacc tcgggggtgt tgggtgtgaa ggcccgtcaa atgaaccatc cgtataccta
     2341 tccgggcgga gtgaccgaca atgaaattcg cgcgtggaac cacctggggc tgttcgcgcc
     2401 gggcgtcacc gatacggcaa cgctgccgac gctcgccgcg gccgacgatg ccgcgcgcag
     2461 cttgcaggat cgggcgcgat cgtatttcga tgccaattgc tcgcactgtc accggccggg
     2521 cgttacggtc gcctattttg atgcccgcta caacacgccc ctcgagcagc aggaactcat
     2581 cgatggcccg gtcttgatcg atcagggaat cgaccggccg cgggtgattt cgccgcacga
     2641 tatttggcgt tcgatcgcct tcatgcgcgt gaacaccgtc gaagacattc ggatgccgcc
     2701 tctggcacgc gagaccatcg atcagaaagg cgtgcggctg ttgcgggagt ggatcacgag
     2761 tctgcctggg cgttcggtat tggatccgcc cgccatctcc ccgcagggcg gcactttcgg
     2821 tggaccgact gcggtcacct tgagcgaact cgaaccggga gccgacatcc gctacacctt
     2881 ggatggcagc gtgccaagcc tctccgacaa tcgctacgct ggaccgatca agctcaccgg
     2941 gcccgtggtg ctgcgcgcgc gcgccttcaa agagggcttc acgcgcagca tcaccgtgca
     3001 acaagtcttc atcatcggtc cgtgaacgca cctcacggag tgttggccac gatcgccacg
     3061 ccccacgggc tcgtcccggc cttgacgcgc gcaatttcct tattggccgc aacatccacc
     3121 acggacacat cgttcgaggg gccgttggcc gtgtagagga acttgccgtc gggagacaac
     3181 gccatgcccc agggccgagt acccacctgg atggtactca ggagctcata cgtgtgacta
     3241 tcgagtaccg cgacggtacc gccgcgcccg gtgctgacat agaccttctg cccgtcggcg
     3301 gcgactttca cgctcatcgg ccgcgacccc ggcggcaagg cgatgacctt cgataccttg
     3361 aggttcagcg tatcgatgat gttaagttcg ccggtcgatt ccgagggaat gaaaccaatc
     3421 gcgccccctg gcaagaagtc catgctgcgc ggacgcacgt tcaccttgaa atgtccgacc
     3481 atcgtgtagc cggccgtttc gatggcatac acgtcgccgc ccgcctcgca cgtgatgtag
     3541 aaacgcgcac cgtcgggtgc cgtggcgacc ccctcgggct cctgacctac cgcgataata
     3601 tgttcgacct ttccgctcgc gatgttgatg acgctcgccg ccttcacgtc ctcgttggag
     3661 acatacagct tggtgccgtc cttgctcaag gagaattcct cgggatccga tccgccgctg
     3721 attttccctg tcaacttccg ctgtgcgaca tccaccacgc cgatggcatc cgccgattta
     3781 tcggacttga cgctgtcgtc atcgtcgtcc ttgcctctct cgaagaccgg attgcctttg
     3841 gcgtccaact tgggcggcgc ggcgatcggc gttccgctca gcgccacgta cacggtcttt
     3901 ccgtccgggc tcgcgtgaat gccgcgcgga cgcttgccga gcggaatcgt ggcgagcacc
     3961 tgattgtctt tgccattgat gacggtgacg tcgccggact tctcattgct cacatagacc
     4021 tgatagtttt gcgccgcttg tgcgccaagc ggcgacacca cgagcaacgc cgtccccaac
     4081 agttgaacac gcatagacat cggcattctc cactccagtc ccggccaagc gcgagtatag
     4141 agctttagcg tgctccgtgt gcgccggacg gtttgtcgtg agcggccggg cgcggttgca
     4201 ggcttttcac cacatcgtcc tgcaccaacg agtaggc
//
LOCUS       DASWZT010000299         5946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_12125, whole genome shotgun sequence.
ACCESSION   DASWZT010000299 DASWZT010000000
VERSION     DASWZT010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5946
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12125"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VGE92_08105"
     CDS             <1..457
                     /locus_tag="VGE92_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313825.1"
                     /translation="AGRALSGSIGITSKLPVDQRAALEQLLFFNVNQHRVRVGIQQSI
                     ETYGVPEIYEQDGGLRVRVGDIAEVQTLFAISDFGRPLGVAVFVRSAHERFAVLHLGL
                     EPRLSATPEMNTRVLLKLMHEIRSAARRTRGVERIELVYKDRHAVRLHG"
     gene            474..1088
                     /locus_tag="VGE92_08110"
     CDS             474..1088
                     /locus_tag="VGE92_08110"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEY1313826.1"
                     /translation="MPGEPDRQTLGAQLHADGKRRCAWCPPDPLYMAYHDTEWGVAVH
                     SDQHLFEMLCLEGAQAGLSWLTILRKRENYRRAFDGFDAIKMARYDAARRRRLLQDVG
                     IVRNRLKVDAFIGNAKAYLEIRSSRSFSDYIWQFTDGRVLRRRPLYLGGIAASSAHSD
                     AMSKDLKRRGFRFVGSTICYAFMQAVGIVDEHQRYCWVASKKPA"
     gene            complement(1107..1808)
                     /locus_tag="VGE92_08115"
     CDS             complement(1107..1808)
                     /locus_tag="VGE92_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEY1313827.1"
                     /translation="MIDLYYWPTPNGHKITLFLEETGMPYTIIPVNIGKGEQFRPDFL
                     AISPNNRMPAIVDHTPMERGAPLSVFESGAILLYLADKSERFIAQDLRGRIEVMEWLF
                     WQVGGLGPMAGQNHHFTKYAPQTIPYAVERYVKETNRLYGVLNKRLADREFVAGSYSI
                     ADMAAYPWIVPHEAQGQNLDDFPHLKRWFESIKTRPATLRAYERGAAISTSTTLSEES
                     RKILFGQTAQPRDAE"
     gene            complement(1805..2794)
                     /locus_tag="VGE92_08120"
     CDS             complement(1805..2794)
                     /locus_tag="VGE92_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313828.1"
                     /translation="MLKITLLLVALLAAHGTVAAPKSAGRAEADKYQAWRTAAEAVLI
                     ARADANSLATAAALRYFGSAAGAKGGLPKPEPSALELAARASELAPQSASIGWLHLQL
                     CSVTPSCDIRDVATVLRWVDADNGAAWLQTLATAQKERDSTEVDRALADIARSMHFDF
                     YWNRLVVLMVDALGAARDQLPGGFAASDSARLTMIIGIASAEIIPSLTPLLEACREPA
                     AAAERRELCLRLSKTMQRGDTVVAQLAGFSIERHLVAPDGREARAIAERRRVLDWRMA
                     EAGKFDSPLLPWTKNARARTRLAAMRAKSREEDVCIAILQSHKVALEPPEVHP"
     gene            complement(2812..3420)
                     /locus_tag="VGE92_08125"
     CDS             complement(2812..3420)
                     /locus_tag="VGE92_08125"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEY1313829.1"
                     /translation="MRKILTLTMSGLVTVLPLALTVYVIWWLVHTVEGWLRRALIALH
                     IVSPEHYWPGSGLIAGFLLLLVVGSLVNAYAGRILLKYWDDFLGRIPLVKTLYGGFRD
                     VVSLLPSGSGEKRDLQRVVLARFADVHAIGFVTREDVPAVLNAHGGHDWVTVYFPMSY
                     AFGGYTIYLPRDRIEPLDISVEDAMRLAITAGLTAAGSRASS"
     gene            complement(3445..4365)
                     /locus_tag="VGE92_08130"
     CDS             complement(3445..4365)
                     /locus_tag="VGE92_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313830.1"
                     /translation="MGYQHAARVALAAWTMVAVSADARAGAIAGAVAFPSQRVPSLTV
                     YAAGIDSSRVASVQLAAGQANFTLEVPAGRYLVFSAPRDADAPNIYGAYTQYSLCALH
                     ASGDKCENHTLIPVSISAKSPRASVSIDDWDLTDDVAARIDRMLGIAAANTAPDPDAL
                     SAPRFSEYPSTSFATDAPRVDFEGSELSQQERAVVLRAFQNGPNFAGHLTAVLTRCGP
                     DCSRLVLVDWRSGQVQEPAALADIRGPLPCRSDAGLQLRRDSRLLSISRTRGTRIATE
                     YYVWNQKNAQLVQSGEYLRTLQTYCAAAAH"
     gene            4510..4716
                     /locus_tag="VGE92_08135"
     CDS             4510..4716
                     /locus_tag="VGE92_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004650632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEY1313831.1"
                     /translation="MSSVARVTEIIASSKKSFEDALDSGVKRATKTLKNVAGAWVASQ
                     DVVIVKGKITEYRVRLKVTFVLAE"
     gene            complement(4742..5392)
                     /locus_tag="VGE92_08140"
     CDS             complement(4742..5392)
                     /locus_tag="VGE92_08140"
                     /inference="COORDINATES: protein motif:HMM:NF027851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HEY1313832.1"
                     /translation="MSFILDALKKSELERQRQSVPGLMDTRTLQRRNRLPVWAVALCV
                     LLTVNLLVLTFVLTRRSVPALPAAPAPLAATAQPVAAAPQQHFSPMDAAPVFAPEIPV
                     ADNPNPAPAEPPQAHSAPHLARPDPLLTEQEAKPEDLESLPTINELNLTGAQALPELH
                     LDVHVYSTNPAERFVYINMRKYHEAARLQEGPTVERIRRDGVVLNYQGLRFVLPRQ"
     gene            complement(5401..>5946)
                     /locus_tag="VGE92_08145"
     CDS             complement(5401..>5946)
                     /locus_tag="VGE92_08145"
                     /inference="COORDINATES: protein motif:HMM:NF044767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEY1313833.1"
                     /translation="AMADDKDPCGQAAKAGLSCLEQRGSWAQVRALNRPAILTLTDAR
                     GQRHRVVLSALDDQFATLYLGEDTQRVPVDELSRDWFGEFTVVWKPKTSHTHPLSLGM
                     QGDEVRWLRRSLNALQGGASDPEHGDVYDEELAIAVQNFQREHRLNVDGVAGVQTQVV
                     LDTALAEPGSPLLLPNAARGG"
BASE COUNT         1126 a         1859 c         1907 g         1054 t
ORIGIN      
        1 ggcggggaga gcattgagcg gttcgatcgg aattacatcg aaattgccgg tggatcagcg
       61 tgctgctttg gaacagttgc tgttcttcaa cgtcaaccaa catcgcgtgc gcgtcggtat
      121 ccagcaatcc attgaaacct acggggtgcc ggagatctac gagcaggatg gcggcttgcg
      181 ggtgcgcgtc ggcgacattg ccgaggtaca gacgctgttt gcgatatcgg attttggccg
      241 gcccctgggc gttgccgttt tcgtgcgctc agcccatgaa cgattcgcgg tgctgcatct
      301 cggactcgag ccgcgcctct ccgcgacccc ggagatgaat actcgcgtcc tgctcaaact
      361 catgcacgag atcaggagcg ccgcgcggcg cacgcgaggc gtcgagcgca tcgagttggt
      421 ctacaaggat cgtcacgccg tgcgcctgca cggctaaccc aaggccggcc tgcatgcccg
      481 gtgaacccga ccgacaaacc ctcggagcgc agcttcacgc cgacggcaag cggcgctgcg
      541 cctggtgccc gccggacccg ctgtatatgg cataccacga tacggaatgg ggcgtcgccg
      601 tgcattccga tcagcacctg ttcgagatgt tgtgtttgga aggcgcgcaa gcgggattga
      661 gctggctcac cattctgcgt aagcgggaaa actatcgacg agcgttcgac ggattcgacg
      721 ccatcaaaat ggctcgctac gacgcagcca ggcgcaggcg cctgctgcag gacgtcggca
      781 tcgtcagaaa tcggttgaaa gtcgacgcct tcatcggcaa cgccaaggcc tacctggaga
      841 ttcgttcatc gcgctcgttc agcgattata tctggcagtt taccgacggc cgcgtgctgc
      901 gccgccggcc gctgtacttg ggcggcatcg cagcctcgag cgcgcacagc gatgcgatga
      961 gcaaggactt gaagcgccgg ggtttccgct tcgtcggctc caccatctgc tatgcgttca
     1021 tgcaagcggt cggaatcgtc gacgaacatc aacgctactg ctgggtggcg agcaaaaaac
     1081 cggcgtgagc gaccggcaat cgcccgtcac tcggcatcgc gcggctgcgc agtttgtccg
     1141 aagagaattt tccgcgattc ctcgctgagc gtcgtgctgg tgctgattgc cgcgccgcgc
     1201 tcataggcgc gcagcgtggc gggacgggtc ttgatcgact cgaaccagcg cttgagatgc
     1261 ggaaaatcat cgaggttctg accctgagct tcatgcggca cgatccaagg gtaggccgcc
     1321 atatccgcga tggagtagct tccggcaacg aattcgcggt cggcgagacg cttgttcaat
     1381 actccgtaca ggcgattggt ctccttgacg tagcgctcga cggcatacgg aattgtctgc
     1441 ggtgcatact tcgtgaagtg atgattctgc ccggccatcg gtccgagtcc gccgacctgc
     1501 caaaaaagcc attccatgac ctcgatccgg ccgcgcaaat cttgggcgat gaagcgctcg
     1561 ctcttgtccg cgaggtacag cagaatcgct ccggattcga atacggacag cggcgcgccg
     1621 cgttccatcg gcgtgtgatc gacgatcgcc ggcatgcgat tattcggcga gatggcgaga
     1681 aaatccggcc ggaattgctc gcccttgccg atattcaccg ggatgatggt atagggcatc
     1741 ccggtctctt ccaggaaaag ggtgatcttg tggccgttcg gcgtgggcca gtaatacaga
     1801 tcgatcacgg gtgtacctcc ggcggctcga gcgccacctt atgagattgc agtatagcga
     1861 tgcagacatc ctcttcgcgc gatttggcgc gcatcgcggc gagccgcgtg cgggcgcgcg
     1921 cattcttggt ccagggcagc agcggactgt cgaattttcc cgcctccgcc atgcgccaat
     1981 ccaacacacg ccgccgctcg gcgatcgccc gggcctcacg gccgtccggc gcgaccaggt
     2041 gcctttcgat gctgaaaccc gccaactgcg cgaccacggt atcgccgcgc tgcatggttt
     2101 tcgacaatct cagacacagc tcgcgccgct ccgcggcagc ggccggttca cggcaggctt
     2161 cgagcaaggg tgtgagggac ggaatgatct cggcgcttgc gatcccgatg atcatggtca
     2221 accgggctga gtccgatgcg gcgaaaccgc ccggcagctg atcgcgggcg gcgccgaggg
     2281 catcgaccat caacaccacc aggcggttcc aatagaagtc aaagtgcatg ctgcgcgcaa
     2341 tgtccgcgag cgctctgtcg acttccgtgg aatcgcgctc tttctgcgcg gtggccagcg
     2401 tctgcagcca ggccgcgcca ttgtccgcgt ccacccagcg cagaacggtt gcgacgtcgc
     2461 ggatgtcgca ggacggcgtc accgagcaga gttgcaagtg cagccagccg atgctcgcgc
     2521 tttgcggtgc cagctcgctg gcgcgagcgg ccagttccag ggcggagggt tcgggcttgg
     2581 gcaagccgcc cttggcgccc gccgcagaac cgaaatagcg cagcgccgcg gccgtcgcca
     2641 acgaattcgc atcggcgcgc gcaatcagaa ccgcctcggc cgcggttcgc cacgcctgat
     2701 atttgtcggc ctcggcgcgt ccggcggatt tgggcgccgc gaccgtgccg tgagccgcca
     2761 gcagtgccac gagcagcaaa gtgatcttga gcacggctta tcccaaggtc ttcaactgct
     2821 cgcgcggcta ccggcagcgg tcaggcccgc ggtgatcgcc aatcgcatgg cgtcctcgac
     2881 cgagatatcg agcggctcga tgcgatcgcg cggcagataa atggtgtagc cgccgaacgc
     2941 atagctcatg ggaaaataca ccgtgaccca gtcatggccg ccgtgcgcat tgagcacggc
     3001 cggcacgtcc tcgcgcgtga cgaagccgat ggcatgcaca tcggcgaatc gggcaagaac
     3061 cacacgctgc aagtcgcgct tctcgccgct gccggaaggc agcaggctca cgacatcgcg
     3121 aaacccgccg tacagggtct tgaccagcgg aatgcggccc agaaaatcgt cccaatactt
     3181 gagcagaatc ctgccggcat aggcgttgac caaactgccg acgaccagca acagcagaaa
     3241 cccggcaatc aaccctgaac ccggccaata atgctcggga ctcacgatgt gcaacgcaat
     3301 caacgcgcgg cgcagccagc cttcgaccgt atgcaccagc caccaaatca catagaccgt
     3361 gagtgccagc ggcaacacgg tcaccaggcc gctcatggtc agcgttaaga tcttgcgcat
     3421 ggctcaatca tcgcatgatc gggcttagtg tgccgcagcc gcgcaatagg tctgcagcgt
     3481 gcgcagatat tcaccgcttt gtaccagctg tgcatttttt tgattccaca cgtaatactc
     3541 ggtggcgatg cgggtaccgc gcgtgcgcga gatgctcagc agccggctgt cgcgtcgcaa
     3601 ctgcaggcct gcatcgctgc ggcacggcag gggtccgcgg atatcggcga gcgctgccgg
     3661 ttcctgcact tgaccgctgc gccaatcgac gagcacgagg cggctgcagt ccggcccgca
     3721 gcgcgtcaac acggcggtga ggtggccggc gaaatttgga ccattctgaa acgcgcgcag
     3781 cacgaccgcg cgctcctgct gcgataattc gctgccctcg aaatcgaccc gcggggcgtc
     3841 ggtggcaaac gaggtgctcg ggtattcaga aaagcgcggc gcgctcaagg catcgggatc
     3901 cggagcggta ttcgcggcgg caattcccag catgcggtcg atgcgcgcgg cgacgtcgtc
     3961 ggtcaaatcc caatcatcga tcgacaccga cgcgcgtggc gatttcgcgc tgatgctgac
     4021 tggaatcaag gtatggttct cgcacttgtc gccggatgcg tggagcgcgc acaggctgta
     4081 ctgcgtgtag gcgccgtaga tattgggagc gtcggcatca cgcggggcgg aaaataccag
     4141 gtagcggccg gcgggtacct cgagcgtgaa attcgcctgc cctgcggcaa gctgcacgga
     4201 cgcgacgcgc gagctatcga tgcccgcggc gtaaaccgtc aacgacggca cgcgctgact
     4261 cggaaacgcg accgcgccgg cgatggcgcc cgctcgagcg tccgcgctca ccgcaaccat
     4321 cgtccaggcc gcaagggcaa cgcgcgccgc atgctgataa cccaagaccg cgattctccg
     4381 ctcgaaacat atcgatcggt caacatggta acgacgcgcg gaggtgcagg ctagtggcgt
     4441 cgattgcgcg gctcggtccg cgtctgtcag gatgtcgggg tcaaaaactc aaagatagcg
     4501 gagtcgaata tgtcgtcagt tgcacgagtc acggaaatca tcgcgtcatc caaaaagagc
     4561 ttcgaagatg ccctcgacag cggcgtgaag cgcgccacca agacattgaa gaacgtggcg
     4621 ggcgcgtggg tggcgagcca ggacgtggtc atcgtcaaag gcaagatcac cgagtatcgc
     4681 gtgcgcttga aagtgacctt cgtcttggcg gagtagcgac cgatcggcgg cgcgatcgcc
     4741 attattgccg cggcaggacg aagcgcaggc cctggtaatt caacaccacg ccgtcgcggc
     4801 ggatgcgctc gacggtaggt ccctcctgga gcctggcggc ctcgtgatac ttgcgcatgt
     4861 tgatgtacac gaaccgctcc gccggattgg tcgagtaaac atggacgtcg agatgcagct
     4921 cgggtagcgc ttgcgcgccg gtcaaattca gctcgttgat agtgggcagc gattcgaggt
     4981 cttccggctt ggcttcctgc tccgtcaaca gcgggtcagg acgggccagg tgcggggcgc
     5041 tgtgggcttg cggcggctcg gcgggtgccg ggttggggtt atcggcgacc gggatttccg
     5101 gcgcgaaaac cggcgccgca tccatcgggc tgaagtgctg ctgcggcgcg gccgctaccg
     5161 gctgcgcggt tgccgcgagc ggcgccggcg cggcgggcag agcggggacg ctcctgcgcg
     5221 tcaggacgaa agtgagcacc agcaggttca cggtcaacag cacgcacagc gcaaccgccc
     5281 agacgggcag gcgattgcgc cgctgcagcg tgcgtgtgtc catcaggccc ggcacggatt
     5341 gccgctgccg ttccagctcc gatttcttga gtgcgtcgag aatgaatgac atgcagattc
     5401 ctagccgccg cgcgctgcgt tcggcaacaa caacggcgaa ccgggttccg ccaacgcggt
     5461 atcgagcacc acttgagttt ggactcccgc gacgccgtcg acgttcagcc ggtgttcgcg
     5521 ctgaaagttc tgcacggcaa tcgccaattc ttcatcgtag acatcgccgt gttccgggtc
     5581 cgacgcgccg ccctgcaagg cgttgaggct gcggcgcaac caacggactt cgtcgccctg
     5641 catgcccaag gacaaaggat gggtgtgcga agttttcggt ttccagacga cggtgaattc
     5701 gccgaaccaa tcgcgcgaca actcatccac cgggacgcgc tgcgtgtcct cgcccagata
     5761 taacgtcgcg aactggtcat ccagcgccga cagcaccacc cgatgccgtt gtccgcgcgc
     5821 gtcggtcaaa gtgaggatgg cgggccggtt caaggcgcgt acctgcgccc aggaaccgcg
     5881 ctgctcgagg cacgacaagc ctgccttggc cgcctgtccg caggggtctt tgtcatccgc
     5941 catggc
//
LOCUS       DASWZT010000300         2120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_84546, whole genome shotgun sequence.
ACCESSION   DASWZT010000300 DASWZT010000000
VERSION     DASWZT010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84546"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VGE92_08150"
     CDS             complement(<1..886)
                     /locus_tag="VGE92_08150"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY1313834.1"
                     /translation="MRAGWAVLSALMLARAGAAAAPADVVLLHGKIHTQDADRSVVQA
                     LAVRGNSIVAAGTDQAIAELVGPSTRTIDLGGRVVMPGIIDAHTHPAESAQDLGKCNL
                     HDKPLTPAQIKRRVALCLKAHPGAPSQWFEVVMVDPSGLTLSLADLDSILPDRPMLFS
                     GSDGHTVWANSRALQAAHISAATPDPAGGHIERDSAGRPTGTLRDDAADIAVAAKPAA
                     DLEHEALQLAKAFARMRATGITSVQDAAVDDHLMQIYKRLYDTHRLNMRVRGALLLEN
                     LREPAAALIGKAVEFRNKW"
     gene            complement(886..1452)
                     /locus_tag="VGE92_08155"
     CDS             complement(886..1452)
                     /locus_tag="VGE92_08155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY1313835.1"
                     /translation="MGSPYIVAVGGTLRANSSTERAMRCVLDAAQRCGARTKLISGSA
                     LQLPLYQPDNAERSDAARDLVAELALADGIILGSPGYHGSISGLVKNALDYAEDLRAD
                     MRPYFSGRAVGCIATAGGWPAAVNTLGALRDIVHALRGWPTPLGAAINSTDNVFDEAG
                     ACLVPRIANMLETIAAEVIEFARTVRGP"
     gene            complement(1484..>2120)
                     /locus_tag="VGE92_08160"
     CDS             complement(1484..>2120)
                     /locus_tag="VGE92_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1313836.1"
                     /translation="GYTATWAGLAVAPVGLLALVAAPLVGRNMNRLNLRLAASFAFCV
                     FAFAIFWAATLNDTASFWQFATPRFLQGLGIAFFFLPLNAILLSGVSSSDLASASGLS
                     NFVRTMSGSIATALTVFLWNRRTDYHHAVLTEHIRNSAGAWSSYQSQLADHGVSGTGA
                     FEYVEHVVTTQAMTLGVNDVFHVLGWLYILLVPFIWLAKPPFGARAAATAH"
BASE COUNT          403 a          685 c          696 g          336 t
ORIGIN      
        1 cccacttgtt gcgaaactcg accgccttgc cgatcaaagc ggcggcgggt tcgcgcagat
       61 tctccaacaa caacgcgccg cgcacccgca tgttcaatcg atgggtgtcg tacaagcgtt
      121 tgtagatctg catcaggtga tcgtcgacgg cggcgtcttg caccgaggta atgccggttg
      181 cgcgcatccg cgcgaaggcc ttggcaagct gcaaagcctc gtgctccagg tcggcggcgg
      241 gcttcgccgc cacggcgatg tcggctgcat catcgcgcag ggtaccggtc ggcctgccgg
      301 ccgagtcgcg ctcgatgtgc ccccctgccg gatcgggagt cgcagcggaa atgtgcgccg
      361 cttgcagcgc gcgcgagttg gcccacacgg tgtggccatc ggaaccgctg aacagcatcg
      421 gccggtcggg aagaatcgaa tcaaggtccg ccaagctcaa ggtgagcccc gaagggtcga
      481 ccatgaccac ctcgaaccac tgagacggcg ccccgggatg cgctttgagg cagagcgcga
      541 cccggcgttt gatctgcgcg ggggtcagcg gcttgtcatg gagattgcat ttgccgagat
      601 cctgtgcgct ctccgccggg tgggtatggg cgtcgatgat gcccggcatc acgacccggc
      661 caccgagatc gatcgtgcgg gtgcttggac ccacgagttc ggcgatggcc tgatccgtgc
      721 cggccgccac gatgctgttg ccgcgcacag cgagtgcctg cacgacgctg cggtcggcgt
      781 cctgcgtatg gattttgccg tgtaacagaa ccacgtcggc cggcgcggct gccgcgcctg
      841 cgcgtgccag catgagcgcc gacagcacgg cccaacccgc gcgcattacg ggccccgcac
      901 ggtgcgcgcg aactcgatga cttcggcggc gattgtttcg agcatattcg cgatgcgcgg
      961 cacgaggcat gcgcctgcct cgtcgaatac attgtcggtc gaattgattg cggcgccgag
     1021 cggtgtcggc caaccacgca gcgcgtgcac gatgtcgcgc aaggcaccca gggtgttcac
     1081 cgccgcgggc cagccgccgg cggtggcgat gcagccgacg gcgcggccgc tgaaatacgg
     1141 ccgcatatcg gcgcgtaaat cctcggcata gtccaaggcg ttctttacca gccccgagat
     1201 gctgccgtga tagcccggcg accccagaat aatcccgtcg gcgagcgcca gctccgccac
     1261 cagatcacgt gccgcgtcgc tgcgctccgc attgtcgggc tgatacaacg gcagttgcag
     1321 cgcggatccg ctgatcagct tggttcgcgc cccgcagcgc tgcgcagcgt cgagcacgca
     1381 gcgcatcgcc cgttcggtcg aagaattcgc gcgcagcgtg ccgccgacgg cgacgatgta
     1441 aggtgagccc atacgaaatc ctacaccgga tcggcgcccg tgatcagtgc gccgtggcgg
     1501 cggcgcgcgc gccgaacggc ggcttggcga gccagatgaa cgggacgagc aggatgtaca
     1561 gccatccgag cacgtgaaac acgtcgttga cgcccaaagt catggcctgc gtggtaacga
     1621 catgttcgac atactcgaag gcacccgtac ccgagacgcc gtggtcggcg agttgcgatt
     1681 gataactgct ccatgccccg gcggaattgc gaatgtgttc cgtcaacacc gcgtgatggt
     1741 agtcggtgcg ccgattccac aagaaaacgg tcagcgcggt cgcgatgctg ccggacatgg
     1801 tgcgcacgaa attgctcagg cccgacgccg acgcgaggtc gctcgaggag acgcccgaca
     1861 gcaaaatcgc gttcaacggc aagaaaaaaa atgcgatgcc caggccctgc aggaaacgcg
     1921 gcgtggcgaa ttgccaaaac gaggcggtgt cgttgagagt tgccgcccaa aaaatggcaa
     1981 aggcgaacac gcagaacgca aaactggcgg ccagacgcaa attcagccga ttcatgttgc
     2041 gaccgacgag cggcgccgcg accaatgcca gcaagcccac gggcgctacc gccaatccgg
     2101 cccaagtcgc cgtgtagcca
//
LOCUS       DASWZT010000301        12443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_79788, whole genome shotgun sequence.
ACCESSION   DASWZT010000301 DASWZT010000000
VERSION     DASWZT010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12443
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79788"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1471)
                     /locus_tag="VGE92_08165"
     CDS             complement(146..1471)
                     /locus_tag="VGE92_08165"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEY1313837.1"
                     /translation="MKTVHAYLLCLVAAGGVVGAARSLSAAESAAGEYGPSPMLPEPD
                     KKLIPTLNIAHAKPWTGDAMPQPAPGLAVAPVARNLKHPRWVYVLPNGDILVAESDAP
                     PKPKDAKGISGKVHKLVMKEAGSGSNPSANRITLLRDPAGDGKNVESHVLLQGLNSPI
                     GMALVGDTLYIADSDAVLGVPYKTGDTEVRATPTQLTALPAGEINHHWTKSLIASPDG
                     SKLYVGVGSNSNAGENGIDAEHERAAVWEIDRATGAHRIYASGLRNPCGLAWQPDSGV
                     LWTSVNERDELGDHLPPDYMTGLHPGAFYGFPYSYYGQHVDDRVKTPAPDLVAKAIAP
                     DYALGAHTASLGLAWSGGAKLGANFEHGMFVGQHGSWNRSTRSGYKVIFVKFDGGRPT
                     GLPRDVLTGFLDNHGDAQGRPVGVAIDKNGALLVADDVGGTVWRVSAAQ"
     gene            1640..2032
                     /locus_tag="VGE92_08170"
     CDS             1640..2032
                     /locus_tag="VGE92_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313838.1"
                     /translation="MTDTKSRLDRAFIEKQRRQLTLLRQQILAVRQARVNEQAATNAE
                     ASGRAREYEDDAQKLTSLELQENLNAVDDDRLSNIERALQRIDEGTYGLSVGSGAPIA
                     IERLEATPEALYTIDEQQSRDALRGAAP"
     gene            2029..2547
                     /gene="msrA"
                     /locus_tag="VGE92_08175"
     CDS             2029..2547
                     /gene="msrA"
                     /locus_tag="VGE92_08175"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY1313839.1"
                     /translation="MSASTERAVLAGGCFWGVQDLIRRYPGVISTRVGYSGGDVKNAT
                     YRNHGTHAEAIEILFDPTVITYRKLLEFFFQIHDPTTLNRQGNDRGLSYRSAIFYTSE
                     EQRRIAQDTIADVEASGLWPGRVVTEIAPVGDFWEAEPEHQDYLERYPDGYTCHFIRP
                     NWQLPVRQKLAS"
     gene            complement(2498..3664)
                     /locus_tag="VGE92_08180"
     CDS             complement(2498..3664)
                     /locus_tag="VGE92_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY1313840.1"
                     /translation="MDFNYSNEQIAMQETLRRFIEREYGFERRRELIRSALGFSAEAW
                     SQYAELGLLSLPFPEEFGGLGGTAVDMMLAMELIGRGLVLEPVLSTVVMAGGLIRDAA
                     SETLQRTLLPRVGAGEIKLALACYEPAGRYDLAHVATRASPSGGGWRLSGEKSVVLDA
                     PAADHLLVSARSAGAVDDAQGIAVFLVPRTASGLSLYAYPTQSGGRAADVKLRQVSVA
                     TDALIGTAQGGLELLERAVDKGIAALCAEAVGIIQALNEATLNYLKTRKQFGVPIGKF
                     QALQHRMADMFIAAEQSRSMAINAAVFADSQDATLRRRAVSGAKAYIGQAARMVGQQA
                     VQMHGAMGVVDDVIVSHYFKRLTMIDLSFGDTDYHLGRFSSLISAAPAAANWAG"
     gene            complement(3670..4881)
                     /locus_tag="VGE92_08185"
     CDS             complement(3670..4881)
                     /locus_tag="VGE92_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007150215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY1313841.1"
                     /translation="MDLKYTPAEAAFRAQVRAFLEAELPTDIRDRMRLAKRLQKDDFT
                     RWQKILQRHGWGAVMWPERFGGTGWSVIQQHIFEEERADVGAPPQNAFSLRMLAPVLM
                     AFGNSAQQEYFLPRIIAGEDWWCQGYSEPGSGSDLASLRTSAVRQGDHYLVNGQKTWN
                     TLGQFADWMFCLVRTSSEGRPQQGISFLLIDMKTPGVSVKPITMLDGEHEINEIWFEN
                     VRVPVQNRVGEENKGWTYAKFLLTHERTNNAGIGTCKRALKKLKEIAAAQLAYGRPLI
                     EDARFRDRIAQVELELMALEITNLRVLSATASESRPLGPEVSVLKIKGSEIQQALAEL
                     QMQALGPDALPYLREALEVEWVADPGFAAHYPPYAPPLAGQYFNLRKTTIYAGSTEIQ
                     KNIISQHILGL"
     gene            complement(4916..5914)
                     /locus_tag="VGE92_08190"
     CDS             complement(4916..5914)
                     /locus_tag="VGE92_08190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017341396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEY1313842.1"
                     /translation="MKALLSRLPGGPDTLVLEEIPEPLPEAGQVRIAVRACGVNYPDL
                     LIIQDLYQIKPTRPFAPGAEVAGVVDALGDGVSNVAVGERVLLAPVRSGMAQKAVGLA
                     SSCWQIPDSMPFEEAAALLMTYGTSQHALKDRAQLRPGETLLVLGAAGGVGLAAVELG
                     KAMGARVIAAASSADKLALAREHGADAGVQYPAGALDKTQARALTDQFKAACGAAGAQ
                     VIYDGVGGDYTEAALRAIAWQGRHLVVGFTAGIPKLPLNLPLLKSCQIVGVFWGDFTM
                     RCPALHAANVAALMTLYLEGRIKPAVTERFPLAQGAQAIARLGAREARGKIVVVID"
     gene            complement(5911..7566)
                     /locus_tag="VGE92_08195"
     CDS             complement(5911..7566)
                     /locus_tag="VGE92_08195"
                     /inference="COORDINATES: protein motif:HMM:NF006181.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY1313843.1"
                     /translation="MSDRHFKFWPAHAMHSMIAPATNLFYNAEVSARRFPNKPFLVFY
                     DTPVSFSGFLDEAERIAGFLERQCGVRKGDRVLLLMQNSPQFVIAYYGILRANAVVVP
                     LNPMNLTQEILRYAADAAATTIIVSQELYSRVEPLLGTALNSVIVAAYSDYLKEPTTL
                     AVPDFIAAPRMQIAAPGVSLWHDVLAAAMHPGALSAGPEDLCVMPYTSGTTGVPKGCM
                     HTHRSVMQTAVAGMQWFAVQPDINLMAVAPLFHVTGMQGGMNGPLYAGNTVVMLPRWD
                     RSAAAQCVQRYRISSWTAIPTMIQDFFANPDIAKYDLSSISRLSGGGAAMPAAVAQRL
                     LDLGVVYYEGYGLSETIGATHINPIGHAKKQCLGIPIYDVDSRVVDPVTLAELPPGEV
                     GEIITHGPQVFLGYWNKPQENAQAFVEIDGKRFFRTGDLGRVDEEGYFFMVDRLKRMI
                     NASGFKVWPTEVESLMYQHPAILEACVIGAKDAHRGETVKALIVPRPEWRATIRAQEI
                     IDWCRENMAAYKCPRIIEFVDSLPKSGAGKIMWRELQEREALS"
     gene            complement(7575..8789)
                     /locus_tag="VGE92_08200"
     CDS             complement(7575..8789)
                     /locus_tag="VGE92_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY1313844.1"
                     /translation="MDFSHSDKVRALQEQLGRFMDEHIYPAEPRFDEEMQKNRLDGNP
                     WQATRVMEELKRAAQAANLWNLFLPHSEHGAGLSNLEYAPLCEIMGRSHIAPEACNCS
                     APDTGNMEVLARYATAEQQRRWLAPLLAGKIRSAFAMTEPDVASSDATNIQSSIVRDG
                     DEYVINGRKWWTSGAGDPRCEILIFMGKTNPSAALHSQQSMILVPLNTPGIRRLRQLT
                     VFGYDHAPHGHSQIDFVDVRVPLENLLLGEGRGFEIAQGRLGPGRIHHCMRLIGLAER
                     ALELMCKRAQQRVAFGKRVSEQGVTLERIAEARILIDQARWLVLNAAYMMDTVGNRAA
                     KKEIAMIKVAAPAMACQVLDWAIQVHGGGGVSDDFPLAAAYAGARTLRFADGPDEVHR
                     EQIGKLELGRYR"
     gene            complement(8793..9848)
                     /locus_tag="VGE92_08205"
     CDS             complement(8793..9848)
                     /locus_tag="VGE92_08205"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY1313845.1"
                     /translation="MSSPAAGLRPPPAQHAFDTGRLDAWMREHVAGFESPIDVQQFAG
                     GQSNPTYLVQSAQRRYVLRRKPPGKLLPSAHAVDREYRLLVALAATDVPVAHAYALCE
                     DPAVIGTAFYVMDYVEGRLFWDAALPEVQAPERAAYYQEMTRVIAALHSVDYAALGLA
                     DFGKPGRYIERQVARWTQQYRASQTETIDAMDRLIEWLPQHIPADEETAIVHGDFRLD
                     NAIFHPHEPRILAVLDWELSTLGHPLVDLAYLCMRYHLSAAQFRGLGGLDLAALRIPS
                     EDECVADYCRRRRRVPVAAADWTYYLAFNMFRLTGILQGVLARAIQGNASSATALEAG
                     RRARPLAEQAWALIDKG"
     gene            complement(9877..10647)
                     /locus_tag="VGE92_08210"
     CDS             complement(9877..10647)
                     /locus_tag="VGE92_08210"
                     /inference="COORDINATES: protein motif:HMM:NF006070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY1313846.1"
                     /translation="MGVRELFELNGKVALITGGSRGLGLQIAQGLGEMGAKIAISARK
                     AGELEEAKRHLAACGIDALTVVNDLTKTEQVPALVETVAGHYGGIHILVNNAGASWGA
                     PAEEHPIEAWNKVMALNATGVFVLSQAVAKRCFIPQRSGNIIVVASVAALRTSMMMKS
                     AAYFASKAAALHLTRALAVEWGVYGIRVNAICPGFFPSKMASALLETISAAVIEHTPL
                     GRLGSDTDLMGAAVYLASDASRHVTGQYIAVDGGASAA"
     gene            complement(10649..11827)
                     /locus_tag="VGE92_08215"
     CDS             complement(10649..11827)
                     /locus_tag="VGE92_08215"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF005494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY1313847.1"
                     /translation="MTEAVIVSTARTGLGKSWKGAFNMTYGATLGAHSVRHAVQRAGV
                     DPAEVEDVIMGSTFGEGTTGGNIARAIALRAGLPVTTAGLSINRFCSSGLQSIALAAQ
                     RILTEGASVIVAGGVESISCVQNETNTHMLRDPWVSEHKPTLYWSMLQTAETVAKRYN
                     ISKEAQDEYGVRSQLRAAAAQAAGKFKDEIVPMTTLMGVADKASGSLGTREVTIASDE
                     GIRADTTLAAVAQIRPAIAGGVITAGNASQFSDGSSAVVVMDRKLAEQRGLKPLGVFR
                     GFAVAGCEPDEMGIGPIFAVPRLLQRTGYKVSDIDLWELNEAFACQVLYCRDKLGIPD
                     ERLNVNGGAIAVGHPYGVSGARLTGHALIEGKRRGAKLVVVTMCIGGGQGAAGLFEVA
                     "
     gene            complement(11824..>12443)
                     /locus_tag="VGE92_08220"
     CDS             complement(11824..>12443)
                     /locus_tag="VGE92_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEY1313848.1"
                     /translation="ALPQQVDAALQEFGMAMGPFRVGDLAGLDIGWAARKRLSAEYPD
                     KDFSNVSDVLCEAGRFGQKTGAGWYRYEAGSRNPIPDPKVTQIIEEYRRKKGFTARQV
                     SSAEIVERCIYALINEGARIIEDGIAQRSSDIDLVYLNGYGFPPYRGGPMFYADQTGL
                     QDIVHALQKIAAQPGSDPSAWKPAALLTRLARQGQTFSDYKGSAK"
BASE COUNT         2250 a         4080 c         3827 g         2286 t
ORIGIN      
        1 gtcgatgccc tcatatattg cgccaatttg cgcattggta aagtgccaat tgttcgagag
       61 aagattgctt ccttgatagg cggaatagcc ccacaagcga aatttaggta agtgaccgat
      121 tcagcgtctg tgccgcgttg cgtgttcatt gagcggcgct cacgcgccac accgtacccc
      181 cgacatcgtc cgccaccagc agcgcgccat tcttgtcgat ggcgacgccc accggccggc
      241 cttgcgcatc gccatggttg tccagaaatc cggtcagcac gtcgcgcggt aggccggtcg
      301 gtcgaccgcc gtcgaatttg acgaaaataa ctttgtagcc gctgcgcgtc gagcgattcc
      361 acgagccgtg ctgaccgacg aacattccat gctcgaagtt cgcgcccaac ttggcacctc
      421 ccgaccacgc gagccccaac gaggcggtgt gcgcgccgag ggcgtagtcc ggcgcgatcg
      481 ccttcgccac caggtccggt gcgggggttt tgacccggtc atcgacatgt tgtccgtagt
      541 agctatacgg aaaaccataa aacgcgcccg ggtgcagtcc ggtcatgtag tcgggtggca
      601 gatggtcccc caactcgtcg cgctcattta ccgacgtcca cagcactcca ctgtccggtt
      661 gccacgccag gccgcagggg tttcgtaaac ctgaggcgta aatccgatgc gcgccggttg
      721 cgcggtcgat ctcccacacc gcggcacgtt cgtgctccgc gtcgatgcca ttctcgcccg
      781 cgttgctgtt ggagccgacc ccgacgtaga gcttgctgcc gtcgggactt gcgatcaggc
      841 tcttcgtcca gtggtgattg atctcgccgg cgggcagcgc ggtgagctgc gtcggcgttg
      901 cgcgcacttc ggtgtcaccg gtcttgtacg gaacgccgag caccgcatcg ctatcggcga
      961 tgtagagcgt gtcccccacg agggccattc cgatcggcga attcaagccc tgcaacagaa
     1021 catggctttc gacattttta ccatcgcccg ctggatcgcg cagcaacgtg atgcgattgg
     1081 cgctcggatt ggacccggag ccggcttcct tcatgaccag cttgtgcact ttgccgctga
     1141 tacctttggc gtccttgggc ttcggcgggg catcgctctc ggccaccaag atatcgccgt
     1201 tcggcagcac gtacacccag cgcggatgct tcagattgcg tgccaccggc gcgacggcaa
     1261 gtcccggcgc cggctgcggc atggcgtctc ccgtccacgg tttcgcatgc gcgatgttca
     1321 aggtgggaat gagcttttta tcgggctccg ggagcatggg tgagggaccg tattcccctg
     1381 ctgccgattc ggctgcgctg agcgaccgag cggcgccgac tacgccgccc gcggccacca
     1441 ggcacagtaa gtacgcgtgc acagtcttca tgacgcgagg ctagtcatga aacggcggcc
     1501 acataatgct cgcggccgac gattgtgcgt cagcgtttcc ctactcgcgg gcaaacctca
     1561 aatcacatcc cccgcgccat cgaccccgct cacagtgtat tatcgcaacg ccggggtttt
     1621 aggaaacacg gggtaactca tgaccgacac gaaatcacgt ttggatcgcg ccttcatcga
     1681 gaaacagcgg cgccagctca ccctgctgcg ccagcaaatt ctggcggtga ggcaggcacg
     1741 cgtcaacgaa caggcggcga ccaatgccga ggccagcggc cgcgcgcgcg agtacgaaga
     1801 cgacgcgcaa aaattgacct cgctcgagct tcaagagaac ctcaacgcgg tcgatgatga
     1861 ccgcctgagc aacatcgagc gcgccttgca aaggatcgat gagggtacgt acggcctgtc
     1921 ggtgggcagc ggcgcaccca tcgccatcga acggctcgag gccactcccg aagcgctcta
     1981 caccatcgat gagcaacaat cgcgcgacgc gctgcgcggg gcggcaccat gagtgcatcg
     2041 acggagcgcg cggttctcgc cggcggctgc ttctggggtg ttcaggacct gatccgccgc
     2101 tatcccggcg tgatttccac gcgcgtcggt tattccggtg gcgacgtcaa gaacgccacc
     2161 taccgcaatc acggcacgca tgccgaagcg atcgaaatcc tcttcgatcc gactgtcatt
     2221 acctatcgca agctgctgga gttcttcttc cagatccacg acccaaccac gctcaaccgc
     2281 caaggcaatg accgtggatt gagctatcgt tcggcgatct tctatacgag cgaggaacag
     2341 aggcgcatcg cacaggacac gattgccgac gtcgaggcgt ccggcctctg gcccggccgc
     2401 gtggtcaccg agattgcacc ggtgggggac ttttgggaag ctgagcctga gcatcaagac
     2461 tacttggagc gatatcccga cggctacacc tgtcatttca tccggcccaa ttggcagctg
     2521 ccggtgcggc agaaattagc gagctgaagc gtcccaggtg atagtcggtg tcgccgaagc
     2581 tcaaatcgat catcgttaga cgcttgaaat agtggctgac gatcacatcg tcgaccacac
     2641 ccatcgcgcc gtgcatttgc acggcttgct gtccaaccat ccgcgccgcc tggccgatgt
     2701 aggccttggc ccctgacact gcgcggcggc gcagcgtcgc atcctgcgaa tcggcgaaca
     2761 ccgccgcatt gatggccatc gaacgcgatt gttcggcggc aatgaacatg tccgccatgc
     2821 ggtgctgcag cgcctgaaac ttgccgatgg gcacgccaaa ctgcttgcgt gtcttcagat
     2881 agttgagcgt cgcctcattg agcgcctgga ttatccctac cgcctcggcg cacagcgcgg
     2941 caatgccctt gtcgaccgca cgctcgagca gctcgagccc gccctgcgcc gtgccgatca
     3001 acgcgtcggt agcgacgctg acttgccgaa gcttgacatc ggcggcacgg ccgcccgatt
     3061 gagtgggata ggcgtacaga ctcagacccg aggcggtgcg cggcaccaag aacacggcga
     3121 ttccctgcgc atcgtcgacc gcaccggcgc tgcgcgccga taccagcaaa tgatccgccg
     3181 caggcgcgtc gagcaccacg gacttctcgc ccgacaggcg ccagccgccg ccgcttgggc
     3241 ttgcgcgagt tgcaacgtgg gccaggtcat aacgccccgc cggctcgtag cacgccagcg
     3301 caagcttgat ttccccggcg ccgacccgcg gcagcaaggt tctctgcagc gtctcggacg
     3361 ccgcatcgcg gatcaagccg ccggccatca ccacggtgct caatacgggt tcgagcacga
     3421 gcccgcgccc gatgagttcc atcgccaaca tcatgtcgac ggccgtgccg ccgagcccgc
     3481 cgaactcctc cggaaacggc agtgacaaga ggccgagctc ggcatactgc gaccacgcct
     3541 cggcggaaaa gcccaacgcc gagcgaatga gttcgcgccg ccgctcgaag ccatactcgc
     3601 gctcgatgaa gcgccgcagg gtctcctgca tggcgatttg ttcgttgctg taattgaaat
     3661 ccatggccgt cacagaccca ggatgtgctg cgagatgatg tttttctgga tttcggtcga
     3721 gccggcgtaa atcgtggtct tgcgcaagtt gaaatactga cccgcgagcg gcggcgcgta
     3781 gggtgggtag tgggctgcga atccggggtc cgccacccac tccacctcga gcgcctcgcg
     3841 cagatacggc agtgcatccg gtcccaacgc ctgcatttgc agctcggcga gcgcctgctg
     3901 aatctccgac cctttgattt tgagcaccga cacttccggt cccaacggcc gtgattcgct
     3961 cgcggtggcc gacagcacgc gcaagttggt gatctcgagc gccatcaatt cgagttcgac
     4021 ctgggcgatg cggtcgcgga agcgcgcatc ctcgatcagc ggccggccgt aggcgagttg
     4081 cgcggcggcg atttctttga gcttcttcaa tgcgcgtttg caggtgccga taccggcatt
     4141 gttggtgcgc tcatgcgtca gtaggaactt cgcataggtc catcccttat tttcttcgcc
     4201 gacccgattt tgcacgggga cacggacatt ctcgaaccag atctcattga tttcgtgctc
     4261 accatcgagc atggtgatgg gcttgaccga gacgccggga gttttcatgt cgatcaacaa
     4321 gaacgaaatg ccctgctgcg gacgcccctc gctgctggtg cgaaccaggc agaacatcca
     4381 atcggcgaac tgccccaggg tgttccaggt tttctggccg ttgacgagat agtgatcgcc
     4441 ctgccgtacg gcgctggtgc gcaatgacgc cagatcggat ccggatcccg gctccgagta
     4501 cccttggcac caccaatcct cgccggcaat gatcctcggc aggaagtatt cctgctgcgc
     4561 ggagttgccg aacgccatca gcacgggcgc cagcattcgc agtgaaaatg cgttctgcgg
     4621 cggcgcaccg acatccgccc gctcctcctc gaaaatatgt tgctgaatca cgctccagcc
     4681 ggtgccgccg aaacgctccg gccacatcac cgcgccccag ccgtggcgct gcagaatttt
     4741 ctgccaacgg gtgaaatcgt ccttctgcag ccgcttggcc aggcgcatgc ggtcgcggat
     4801 atcggtgggc agctcggcct cgaggaaggc tcgcacctgc gcacgaaatg ccgcttcggc
     4861 gggcgtatat ttcaagtcca tgcgaactcc ccccggcacc cctcaaaggc gaccgtcaat
     4921 cgatgaccac cacgattttg ccgcgcgcct ctcgggcgcc gagccgcgcg atcgcttgcg
     4981 caccctgggc caacggaaat cgctcggtga ccgcaggctt gatccggcct tcgaggtaga
     5041 gcgtcatcag cgccgcgaca ttggctgcat gcagtgccgg acagcgcatg gtgaagtctc
     5101 cccagaaaac gccgacgatt tgacagctct tgagcagcgg cagattcagc ggcagcttcg
     5161 gaatcccggc cgtgaagccg acgactaagt gccgcccctg ccaggcgatc gcgcgcagcg
     5221 ccgcctccgt atagtctccg cccacgccgt catagatgac ttgcgcaccg gcagcgccgc
     5281 acgcagcttt gaattggtcg gtcagcgcgc gcgcctgagt cttgtcgagc gcaccggccg
     5341 gatattgaac gcctgcatcg gcgccgtgtt cgcgcgccaa ggccagctta tcggcgctcg
     5401 atgcggccgc gatgacgcgc gcgcccatcg ccttgcccaa ctcgaccgcc gcgagaccga
     5461 ccccgcccgc cgcaccgagc accagcagcg tttcgccggg ccgcaattgc gcccgatctt
     5521 tgagcgcatg ttgcgaggta ccataggtca tgaggagcgc cgcggcttcc tcgaacggca
     5581 tgctatcggg gatctgccaa cagctgctgg cgagccccac ggctttctgc gccatgccgc
     5641 tgcgcaccgg agcgagcagt acgcgctcgc cgacggccac gttgctcacg ccgtcgccga
     5701 gcgcatccac cacgccggca acttctgcac ccggcgcaaa cgggcgcgtc ggcttgattt
     5761 ggtagaggtc ctggatgatc aacagatccg gataattcac accgcaggcg cgcacggcaa
     5821 ttcgcacttg gccggcttcg ggaagcggct cgggaatctc ctcgagcacc aaggtgtcgg
     5881 gaccgccggg aagtcgactg agcagcgctt tcatgagagg gcttcgcgtt cctgcagctc
     5941 gcgccacatg attttaccgg cgcccgactt gggtagcgaa tcgacgaact cgatgatccg
     6001 cggacattta taggccgcca tattctcgcg gcaccagtcg atgatctcct gtgcccggat
     6061 agttgcgcgc cattcgggcc tcggcacgat cagcgccttg accgtctcgc cgcggtgcgc
     6121 gtctttggcg ccgatgacgc aggcctcgag gatcgccgga tgctgataca tcaaggattc
     6181 gacctcggtg ggccatacct tgaatcccga ggcgttgatc atgcgcttca ggcgatccac
     6241 catgaagaag tagccttcct catccactcg cccgagatcg ccggtgcgaa agaaacgctt
     6301 gccgtcgatc tcgacgaacg cctgggcatt ctcttgcggc ttattccaat accccaagaa
     6361 cacttgcggg ccgtgcgtga tgatttcgcc gacctcgccg ggcggcagtt ccgccaaggt
     6421 cacaggatcg acgacgcgcg agtccacatc gtagatcggg atccccaagc actgcttctt
     6481 ggcgtgaccg atgggattga tgtgtgtcgc gccgatcgtt tcggacaagc cgtagccttc
     6541 gtaataaacg accccgaggt ccaacaatcg ctgcgcgacg gcggccggca tggcggcgcc
     6601 gccgccgctc aggcggctga tggaactcaa gtcgtatttc gcgatatccg gattcgcaaa
     6661 aaaatcctgg atcatggttg gaatcgccgt ccagctcgaa attcggtagc gctgcacgca
     6721 ctgcgccgcg gcgctgcgat cccagcgcgg cagcatcacc accgtattgc cggcgtacag
     6781 gggcccgttc atgccgccct gcatgccggt tacgtgaaac aagggagcga cggccatcaa
     6841 gttgatgtcc ggttgcacgg cgaaccactg catgccggcg accgcggtct gcatcacgct
     6901 gcggtgcgtg tgcatgcagc ccttcggcac tccggtggtg ccggaggtat agggcatgac
     6961 gcacagatct tcgggaccgg cgctcaaggc gccggggtgc atcgcggcgg cgagcacgtc
     7021 gtgccacagc gacacaccgg gcgccgcgat ttgcatgcgg ggcgcggcga tgaagtcggg
     7081 caccgctaaa gtggtcggct ctttgagata gtccgagtaa gccgcgacga tcaccgagtt
     7141 gagcgccgtg ccgagcagcg gttcgacgcg cgagtacagc tcctgcgaga cgatgatggt
     7201 ggttgccgcc gcgtccgcgg cgtaacgcag gatttcctgc gtcaggttca tcggattcaa
     7261 gggcacgacg acggcgttgg cccgcaaaat gccgtagtac gcgatgacga attgcggact
     7321 gttctgcatc agcagcagca cgcgatcccc tttgcgcacg ccgcactgcc gctcgaggaa
     7381 cccggcgatg cgctctgcct cgtccaagaa tcctgagaag ctcaccggcg tgtcgtagaa
     7441 caccaggaac ggcttgttgg gaaagcggcg cgccgacacc tcggcgttgt agaagaggtt
     7501 ggtcgccggt gcgatcatgc tgtgcatcgc atgcgccggc cagaatttga agtgccgatc
     7561 ggacatggcg ttgactagcg gtagcgaccc agctccagct tgccgatctg ttcacggtgc
     7621 acttcgtccg gcccatccgc gaatcgcagc gtgcgtgcac cggcgtaagc agccgccagc
     7681 ggaaagtcgt cgctgacgcc gccgccgccg tgaacttgga tcgcccaatc gagcacctgg
     7741 caggccatgg cgggcgctgc caccttgatc atggcgatct ctttcttggc ggcccggtta
     7801 ccgacggtat ccatcatgta ggccgcattc agcaccagcc agcgcgcctg atcgatcagg
     7861 atgcgcgctt cggcaatgcg ctcgagcgtg acgccctgct ccgacacgcg cttgccgaag
     7921 gcgacacgct gctgggcgcg cttgcacatc aattcgagcg cgcgctcggc gagcccgatc
     7981 aggcgcatgc aatgatggat gcgccccggt cccaagcggc cctgtgcaat ctcgaaaccc
     8041 cgtccctcgc ccagcaacag attctcgagc ggcacgcgca catcgacgaa gtcgatctgc
     8101 gaatgaccgt gcggcgcatg gtcgtagccg aagacggtga gttgccgcag ccgccggatg
     8161 ccgggcgtgt tcagcggcac gagaatcatc gactgctggc tgtgcagcgc cgcgcttgga
     8221 ttggtcttgc ccatgaaaat caagatctcg cagcgcggat cgccggctcc ggaggtccac
     8281 catttgcggc cattgatcac gtactcgtcg ccgtcgcgga cgatggagga ttggatattg
     8341 gtggcatcgc tcgaggcgac gtcgggttcg gtcatcgcaa aggcggagcg aatcttgccc
     8401 gcaagcagcg gcgccagcca acgtcgttgt tgctcggccg tggcatagcg agccaacacc
     8461 tccatgttgc cggtgtcggg agccgagcag ttgcaggcct cgggcgcaat gtgcgagcgg
     8521 cccatgatct cgcacagcgg cgcgtattcg agattggaca gcccggcgcc gtgctcggag
     8581 tgcggcagga acaagttcca gagatttgcg gcctgcgcgg cgcgcttcaa ttcctccatc
     8641 acccgggtgg cctgccaggg gttgccgtcg aggcgattct tctgcatctc ctcatcgaag
     8701 cgcggctccg ccggatagat gtgctcatcc atgaaacgcc cgagttgttc ctgcagggcg
     8761 cgaaccttgt cgctgtgtga aaaatccatg tgttatccct tgtcgatgag tgcccaggct
     8821 tgttcggcca gggggcgcgc gcgccggccc gcctccaacg cggtggcgct cgacgcgttg
     8881 ccttgtatgg cgcgcgccag cacgccctga agaattcccg tcaggcgaaa catgttgaat
     8941 gccagataat aggtccagtc cgccgcggcc accggcacgc gccggcggcg ccggcaataa
     9001 tcggccacgc attcatcttc gctcggaatg cgcagcgctg cgaggtccaa tccgccgaga
     9061 ccgcgaaatt gcgcggccga taggtggtag cgcatgcaga gatacgccag atcgacgagc
     9121 ggatgtccga gcgtcgacag ttcccagtcg agcacggcga gaattcgcgg ctcgtgcggg
     9181 tgaaagatcg cgttgtcgag acggaaatcg ccgtgcacga tcgcggtttc ctcgtccgcg
     9241 gggatgtgtt gcggcaacca ctcgatcaag cgatccatgg catcgatggt ctcggtctgg
     9301 gatgcgcggt attgctgggt ccagcgcgcc acttggcgct cgatgtagcg gccgggcttg
     9361 ccgaaatccg ccaaaccgag cgccgcgtaa tccaccgagt gcaacgcggc aatcacgcgc
     9421 gtcatctcct ggtagtacgc ggcgcgctcg ggcgcttgca cctcaggcag tgcggcatcc
     9481 caaaaaaggc gcccctcgac gtagtccatc acatagaacg cagtgccgat caccgcgggg
     9541 tcctcgcaca gcgcgtaagc atgggccaca ggcacgtccg tcgccgccaa cgccaccagc
     9601 aggcggtatt cgcgatcgac cgcgtgcgcc gatggcagca gcttgcccgg gggcttgcgc
     9661 cgcagcacgt aacgacgctg cgccgactgc acgagatacg tcggattcga ttgtccgccc
     9721 gcgaattgct gcacgtcgat gggactttcg aatcccgcaa cgtgctcccg catccaggca
     9781 tcgaggcgac ccgtgtcgaa ggcgtgctgt gccgggggcg gccgcaagcc tgcggccggg
     9841 ctgctcacgc tgcgcccttg agtcgcatcg cgcgattcat gccgcggacg cgccgccgtc
     9901 cacggcgatg tactggccgg tcacgtgacg cgatgcgtcc gaggcaagat agaccgcggc
     9961 gcccatgagg tcggtatcgc tgccgagacg cccgagcggc gtatgctcga tcacggccgc
    10021 ggaaatcgtc tcgagcagtg cactggccat tttcgatggg aaaaaccccg ggcagattgc
    10081 attgacgcgg atgccataca ctccccactc caccgccagc gcgcgggtca gatgcaaggc
    10141 ggcggctttc gaggcgaagt aggcggccga tttcatcatc atgctggtgc gcagcgccgc
    10201 gaccgaggcg accacgatga tattgcccga gcgctgcgga atgaaacacc gcttggcgac
    10261 tgcctggctc aatacgaata cgccggtcgc attcagggcc atgaccttgt tccacgcctc
    10321 gatgggatgc tcttcggcgg gcgcgcccca gctcgcgccg gcgttgttga ccagaatgtg
    10381 aatgccgccg tagtggccgg cgaccgtctc gacgagcgcc ggcacctgct ccgtcttcgt
    10441 cagatcgttg accaccgtca gcgcatcgat gccacaagcc gcgaggtggc gcttggcttc
    10501 ctcgagctcg ccggccttgc gcgcactgat cgcaatcttc gcgcccatct cacccaagcc
    10561 ctgcgcgatc tgcaaaccca acccacgcga gccacctgtg atcaaagcga ccttgccgtt
    10621 gagctcgaac agttcgcgca ctcccatctc aagcgacctc gaacagtccg gcggctccct
    10681 ggccgccgcc gatgcacatg gtcaccacca cgagtttggc gccgcggcgc ttgccttcga
    10741 tgagcgcatg tcccgtcagg cgcgcgccgc tgacgccgta gggatggccg accgcaatgg
    10801 cgccgccgtt gacgttcaag cgctcgtcgg gaatgccgag cttgtcgcgg caatacaaaa
    10861 cctggcaggc aaaggcctcg ttcagctccc acagatcgat gtcgctcacc ttgtagccgg
    10921 tccgctgcaa caggcggggc acggcaaaaa tcggaccgat gcccatttca tcgggttcgc
    10981 agccggcgac ggcaaagccg cgaaaaacac ccaacggttt caagccgcgc tgttccgcca
    11041 acttgcgatc catcacgacg acggcgctgc tgccgtcgga gaactgactg gcattgccgg
    11101 cagtgatgac gccgcccgcg attgcggggc ggatctgcgc cacggccgcg agcgtggtgt
    11161 ccgcccggat cccctcatcg ctggcgatgg tcacttcgcg cgtgccgagc gaacctgagg
    11221 ctttgtcggc gacgcccatc agcgtcgtca tgggcacgat ttcgtccttg aatttgccgg
    11281 cggcctgggc ggcggcggcg cgtagctggc tgcgaactcc gtactcatct tgagcttcct
    11341 tgctgatgtt gtagcgcttc gccacggtct cggcggtttg cagcatgctc caatacagcg
    11401 tcggcttgtg ctcgctcacc cagggatcgc gcagcatgtg cgtgttcgtc tcattctgca
    11461 cgcaggagat gctttcgacg ccgccggcga cgatcacgct cgcgccttcg gttagaattc
    11521 gttgcgccgc caaagcgatg gattgcaatc cggagctgca gaagcggttg atgctcagtc
    11581 ccgcggtggt gacgggaagt ccggcgcgca gcgcaatcgc acgcgcgatg ttgcccccgg
    11641 tggtgccttc accgaaggtc gagcccatga tcacatcctc gacttcggcc gggtccacgc
    11701 ccgcacgctg caccgcgtga cgcaccgaat gcgcgcccaa cgtggcgccg taagtcatgt
    11761 tgaaagcacc cttccagctc ttgccgaggc cggtgcgcgc cgtcgagacg atgaccgctt
    11821 ctgtcacttg gctgatcctt tgtagtcact gaaggtttga ccttgacgcg ccaaccgggt
    11881 gagcagcgcc gcgggcttcc atgcgctcgg atcgctgccg ggctgcgctg cgattttttg
    11941 caaggcgtgc acgatatcct gcagacccgt ctggtccgcg tagaacatgg ggccgccgcg
    12001 gtacggcgga aaaccatagc cgttgaggta caccaaatcg atgtccgagg agcgctgcgc
    12061 gatgccatcc tcgatgatgc gggcgccttc gttgatcagc gcgtaaatgc agcgttcgac
    12121 gatctcggcc gagctgacct gacgcgccgt gaagcccttt ttccggcggt actcctcgat
    12181 gatttgcgtg accttggggt ccggaattgg attgcgcgat ccggcctcgt agcgatacca
    12241 acccgcgccg gtcttttggc cgaaacggcc tgcctcgcac agtacgtcgg acacattcga
    12301 gaaatccttg tcgggatatt ccgccgacag ccgcttgcgc gccgcccagc cgatatcgag
    12361 gccggccaaa tcaccgaccc gaaatgggcc catcgccatg ccgaattcct gaagcgccgc
    12421 atccacttgc tgcggcaatg cgc
//
LOCUS       DASWZT010000302         2098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_50606, whole genome shotgun sequence.
ACCESSION   DASWZT010000302 DASWZT010000000
VERSION     DASWZT010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50606"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VGE92_08225"
     CDS             complement(<1..766)
                     /locus_tag="VGE92_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY1313849.1"
                     /translation="MLSPQWAGSEQAEESSDEALLDAYSGAVIGALERVAPAVTFIEV
                     VHKAAPVGRGATPRGRGAPRANRPARGSGSGFLFTPDGYLLTNSHVVHGSDEITVRLN
                     DDSRFSADLVGNDPDSDLAVLRIGSPNALPFAEFGDSARLKVGQVVIAIGNPLGYSKT
                     VTTGVVSALGRTLRASSGRLMHDVIQTDAALNPGNSGGPLVDSKGRVIGVNTAMIPEA
                     QAICFATGIDTAKWVIGQLFAHGRVRRAYIGVSGATV"
     gene            complement(769..>2098)
                     /gene="htpG"
                     /locus_tag="VGE92_08230"
     CDS             complement(769..>2098)
                     /gene="htpG"
                     /locus_tag="VGE92_08230"
                     /inference="COORDINATES: protein motif:HMM:NF003555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEY1313850.1"
                     /translation="KEFLEPARLRQLVRKYSDHLSIPIVLKDGASAETVNQAKAFWTR
                     PKSELSDEDYQAFYKHLTHDSEAARIWAHNKVEGNIEYTSLLYLPKRAPFDLFEREQK
                     GGIQLYVKRVFIMDKAAELLPPYLRFMRGLVDTADLPLNVSRELLQNNRTVEKIKSAL
                     VKRSLDLLEDLAANKPQEYAEFWKTFGVVLKEGVIDYPIQKERIAKLLRFHSTAATGD
                     APDVTLEQYVSRMQAEQKAIYFLTADSLAAARNSPHLEGFRAKGMEVLLLIDRIDEWV
                     AGHLHEFEGKALANVASSAADVASAIETPEKAAAESAFKDTTERLGKVLNGKIASAQV
                     SARLTDSPAVLVAGEFGMSLRMGRILKQAGQNNPFATLPILELNPKHPLVERLRDTSD
                     EAAFADLAHVLFDQAMLAEGGELDDPAIFVRRVNRLIVEGFAAQPKIILS"
BASE COUNT          384 a          682 c          630 g          402 t
ORIGIN      
        1 gtacggtcgc gccggagacg ccgatatagg cgcggcgcac ccggccgtgc gcgaacagct
       61 ggccgatcac ccacttggcg gtgtcgattc ccgtggcgaa acaaatcgcc tgggcctcgg
      121 ggatcatcgc ggtattgacg ccgatcaccc gccccttgga gtcaaccagg ggaccgcccg
      181 agtttcccgg attgagcgcg gcatcggttt gaatcacatc gtgcatgagc cggccggagc
      241 tggcgcgcag cgtacgtccg agcgcggata cgacgcccgt cgtcacggtc ttcgagtacc
      301 cgagcggatt gccgatggcg atgacgacct ggcccacctt gagacgcgcg gaatcgccga
      361 attcagcgaa cggcagcgcg ttcggcgatc cgattcgcag taccgccaag tccgagtccg
      421 gatcgttgcc gaccaagtcg gcgctgaagc gcgagtcgtc attcaagcgg accgtgatct
      481 catccgagcc atgcacgaca tggctattgg tcaagagata gccgtcgggc gtgaataaaa
      541 aacccgagcc gctgccgcga gcgggccggt ttgcgcgcgg tgcgccgcgt ccccgcggcg
      601 tggcgccccg ccccacgggc gccgccttgt gcaccacctc gatgaatgtc accgcaggcg
      661 ccacccgctc gagcgcgccg atcacggcgc ccgagtacgc atcgagcaat gcctcgtcgg
      721 acgactcctc ggcttgttca gacccggccc actgcggcga cagcatgctc agctcaggat
      781 gattttcggc tgcgccgcga aaccttccac gatcaaccga ttgacccggc gcacgaaaat
      841 cgccggatca tcgagttcac cgccttccgc caacatggcc tggtcgaaga gcacatgcgc
      901 cagatccgcg aaagccgcct catcgctcgt gtccctgagg cgctccacca agggatgctt
      961 cggattgagc tcgagaatcg gcagtgtggc gaacggattg ttctggcccg cctgcttgag
     1021 gatgcggccc atgcgtaggc tcatgccgaa ctcgcccgcg accagcaccg ccggcgagtc
     1081 ggtcagacgc gcggacacct gggcgctcgc gatcttgccg ttcaacacct tgcccagccg
     1141 ctccgtcgtg tccttgaagg cagattccgc agcggctttt tccggagtct caattgccga
     1201 ggcaacgtcg gcggcgctgc tcgcgacgtt cgccagcgct ttgccttcga actcgtgcag
     1261 gtggccggcg acccattcgt caatgcgatc gatcagcagc aaaacctcca tgcccttggc
     1321 gcggaaaccc tccagatgcg gactgttgcg cgcggcagcg agactatcgg cagtcagaaa
     1381 gtagatggct ttctgctcgg cttgcatgcg cgacacgtac tgctcgagcg tgacatccgg
     1441 ggcatcgccc gtggcagcgg tggaatggaa gcgcagcagc ttcgcgatac gttccttctg
     1501 gatcggataa tcgatcacgc cttccttcaa gactacgccg aaggttttcc agaattccgc
     1561 atactcttgc ggtttgttcg cggccaaatc ctcgagcaga tcgagcgagc gcttcaccag
     1621 cgccgacttg attttctcga cggtgcggtt gttctgcagc agttcgcgcg acacattcag
     1681 cggcagatcg gccgtatcga ccagtcctcg cataaatctc aagtagggcg gcagcaattc
     1741 ggctgccttg tccatgatga acacgcgctt gacgtaaagc tggataccgc ccttctgctc
     1801 gcgctcgaac agatcgaacg gcgctcgctt cggcaggtag aggagcgagg tgtactcgat
     1861 attgccctcg accttgttgt gtgcccagat gcgcgccgcc tcggaatcgt gcgtcagatg
     1921 cttatagaac gcctggtagt cttcgtcgct caactccgat tttggacgcg tccagaatgc
     1981 cttggcctgg ttcaccgtct ctgcgctcgc gccgtctttc aagacgatgg gaatgctcaa
     2041 gtgatccgaa tatttacgca ccagttggcg cagccgcgcc ggctcgagaa actccttg
//
LOCUS       DASWZT010000303         2241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_94413, whole genome shotgun sequence.
ACCESSION   DASWZT010000303 DASWZT010000000
VERSION     DASWZT010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94413"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="VGE92_08235"
     CDS             <1..1287
                     /locus_tag="VGE92_08235"
                     /inference="COORDINATES: protein motif:HMM:NF024795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HEY1313851.1"
                     /translation="ASGGTEAVASGATASGGAVYGKLTDLGSATGITVNGGGTLVMSG
                     GKASAVTLDVGGNVDLTTIAYASGDQASVVSGLLTITTSGGSVITTSVGLAGNYAGET
                     FIVTNDGNGGVEVGLCYIRGTRVLTPTGERRIENLKIGDPVVTRFGGIQRIKWIGRQR
                     YVADEVREDLEIIPVHIRAAALGDDLPARDLYISPGHSMLIGGTLVLAKSLVNGITIT
                     QGWARENLDYFQIELDKHDCIVVEGAWSETFADGPGLRERFHNLAEFNAMFPDHPTPD
                     ALVALCAPRPEQGAALAAALRPVVERARTGVASGPLRGFIDRVDGSWKLDGWAQDADH
                     PELPVLLEVLLENKVIGTVLACDFRKDLFDVFGHGNSSFVFESPVKLRGELLRTLQVR
                     RASDGAAVPINENIRPRAEVPANNPAVERERESVKP"
     gene            1320..1535
                     /locus_tag="VGE92_08240"
     CDS             1320..1535
                     /locus_tag="VGE92_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313852.1"
                     /translation="MHTYYAEKKAAAKTESAKRERLRAGDPDISNDELLSIIEKAEAK
                     RAELLSSQPEAKRNERILNALPAAAKG"
     gene            complement(1842..>2241)
                     /locus_tag="VGE92_08245"
     CDS             complement(1842..>2241)
                     /locus_tag="VGE92_08245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY1313853.1"
                     /translation="YLERVAKVGRNVTVRGPYEPERVDELMRNVDLIVVPSIWWENSP
                     LVIEEALRNRRPVVCSDIGGMAEKVRDGQDGFHFPMGNAMALASLLRRIAADRGSLKT
                     ISGLLRAPPDPDRVAAQHRALYSPLHAATA"
BASE COUNT          447 a          663 c          726 g          405 t
ORIGIN      
        1 gcgagcggcg gcaccgaggc ggtcgcatcc ggcgccacgg cgagcggcgg cgccgtttac
       61 ggcaagctca ccgacctggg ttcggccact ggcatcaccg tcaatggcgg cggcacgctg
      121 gtcatgtcgg gggggaaggc gtccgcggtg acgctcgatg tgggcggcaa cgtcgatctc
      181 accaccatcg cgtacgcgag cggcgatcag gcgtcggtgg tcagcgggct gttgaccatc
      241 acgaccagcg gcggcagtgt catcaccacc agtgtaggct tagccggaaa ttacgcgggc
      301 gagaccttca tcgtgacgaa cgatggaaat ggcggtgtcg aagtcggtct ttgctacatt
      361 cgcggaactc gggttttgac gccaaccggc gaacgccgca tcgagaattt gaaaatcggc
      421 gatcctgtgg tcacgcgttt cggcggcatc cagcgcatca agtggattgg ccgtcaacgt
      481 tatgtggcgg acgaagtccg ggaggatctc gagataatcc cggtgcatat acgggccgcc
      541 gcgctcggtg acgatctgcc ggcaagggat ttgtacatct cccccggcca ttcgatgctc
      601 atcggcggca cgctggtgct agccaagtcg ctggtgaatg gtattacgat aacgcagggt
      661 tgggcgcggg aaaacctgga ttacttccaa atagaactcg ataagcacga ttgcatcgtg
      721 gtggaaggcg cgtggtccga gaccttcgcc gacggcccgg ggctgcgaga gaggttccac
      781 aatctggcgg agttcaacgc gatgttcccg gatcatccta ctccggacgc tctcgtcgcg
      841 ctgtgtgcgc cacgaccgga gcaaggcgct gcgctcgctg ccgccttgcg accggtcgtg
      901 gagcgcgccc gcacgggtgt cgcttcgggt ccgctgcgcg gctttatcga ccgtgtcgat
      961 ggttcatgga agctcgatgg ctgggcgcag gatgccgatc atcccgagct gccggtgctg
     1021 ctcgaggtat tgctggagaa caaggtaatc ggtacggtgc tcgcgtgcga tttccgcaag
     1081 gacttgttcg atgtctttgg tcatggcaac tcgagcttcg tgttcgaatc gccggtgaag
     1141 ctgcgcggcg aactgctgcg taccctgcag gtgcgacgcg ccagcgacgg cgcagcagtg
     1201 ccgatcaacg agaacatccg gccgcgcgcc gaggtgccgg cgaacaatcc ggcggtggag
     1261 cgcgagcgcg agagcgtcaa gccatagctt ggtagctcgt ctccgaaatg gtgaaggaga
     1321 tgcataccta ctacgccgag aaaaaggctg ccgccaaaac tgagagcgcc aagcgggaac
     1381 gtctgagggc cggcgacccc gacatatcca acgacgaact gctcagcatc attgaaaagg
     1441 ccgaagccaa acgagcggaa ctgttgtcgt cgcaaccgga agccaagcga aacgaaagga
     1501 tactcaacgc cctgcccgct gcggccaagg gttgatgacc cagaagagag catcatgtga
     1561 tgccggatct tgggccgccc gaattatttc ggcgcgtgga gcgcgagctc ctgactaccg
     1621 gcagttccac agccgcagct cggcgtgctc agcgagggca ttaccaatca tcgagatatt
     1681 gtttgaatgt ggtcaacgca ttcgatcgag agcggacagc atcccttgct gctgcatttt
     1741 gacggaaagg tcggcgacaa actctacggt agaccgagtc cgtcggcggg atcgaaacaa
     1801 atcggcatcc cgccagtacg gccttttccg agttgctaag actaggccgt cgcggcgtgc
     1861 agcggcgagt acagtgcgcg gtgctgggcc gcgactctgt ccggatcggg cggcgcgcgc
     1921 agcagtcctg agatggtctt caacgaaccg cgatcggctg cgattcgccg cagcagcgag
     1981 gccaaggcca tggcattgcc catgggaaag tgaaagccat cctgaccgtc gcgcactttc
     2041 tcggccatgc cgccgatatc cgagcagacg accggccgcc ggttgcgtag cgcctcttcg
     2101 atcaccagcg gcgaattctc ccaccagatg ctcgggacta cgatgaggtc cacattgcgc
     2161 atcaactcat cgacacgctc cggttcgtac ggaccgcgca cggtaacatt gcggccgacc
     2221 ttggccacgc gttccaaata c
//
LOCUS       DASWZT010000304         1752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_60441, whole genome shotgun sequence.
ACCESSION   DASWZT010000304 DASWZT010000000
VERSION     DASWZT010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60441"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(326..1354)
                     /locus_tag="VGE92_08250"
     CDS             complement(326..1354)
                     /locus_tag="VGE92_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY1313854.1"
                     /translation="MNYYAGIDVSLEYSSVCVLDGSGKIVREGKVLSEPDALIAWFGG
                     LGFGLERIGLEAGPLSQWLFAAMTQAGLAVELLETRHVRNAFKTMPVKTDRRDARGIA
                     ELMRLGWFRPVHCKSAEAQETRALLTARKLVQTKLHDIELSLRGILRGFGLKVGPTTP
                     TQFEARITELVESHPSLQVIAQSLLAVRTVLLREFNGFEKRVRSMARSDARTRLLMST
                     PGVGTIVALTYVSAIDDPKRFKSSKAVGSHFGLTPKKYQSGETDITGKISKIGDAGVR
                     AALYEAAHVILTRPVKGSNLKSWAARIAKRAGMKTAKVALARKLAVIMHRMLVDAKPF
                     NFAAAAAR"
BASE COUNT          314 a          563 c          521 g          354 t
ORIGIN      
        1 ggcgacgacg agttcggatt ttattgaaca gtttcatctg cgatcccagg aaacggttgg
       61 gctagaatcc gcccttctgt aacgggcctc ggtttgtcaa tccctttttg catcatccgt
      121 cgccaggcac tcgcttctgt acggggtcgg tgcaagaccg ccacctgatg gggtgctgag
      181 aggaaaaggc cgaccgatct acttacgcgt ggtcgcaatg ccactgggcg gattgcggtc
      241 tgacctgatc catcgtcccg gctagggact ccgcttcggg aaggctggtg tccgaaaccc
      301 gcccgaaatc gtcgaaactc tccttctatc gcgccgccgc ggcggcgaag ttgaacggct
      361 tggcatcgac cagcatgcga tgcatgatga ccgccagctt gcgggccagt gccaccttgg
      421 cggttttcat gccggcgcgc ttggcgatcc tggccgccca gcttttgagg tttgatccct
      481 tgaccggccg agtcaggatg acgtgagccg cctcatagag cgcggcgcgc acgcccgcgt
      541 caccgatctt cgagattttt ccggtgatgt cggtctcgcc ggactgatat ttctttggcg
      601 tcagtccgaa atgagaaccg accgccttgg aggacttgaa gcgtttggga tcgtcgattg
      661 cggaaacata ggtcagcgcc acgatcgtgc ccacgccggg cgttgacatc aatagccgcg
      721 tccgcgcatc cgagcgtgcc atcgaccgca cccgtttctc gaaaccgttg aactcgcgca
      781 gcagcaccgt gcgtacggcc agcaacgact gggcgatgac ctgcaagctc ggatggctct
      841 cgacgagctc cgtgatcctg gcctcgaact gcgttggcgt ggtcggtccg accttgaggc
      901 cgaagccacg cagaatgccg cgcaggctga gctcgatatc gtgaagcttg gtctgcacca
      961 gtttgcgcgc cgtcagcagc gcccgggtct cttgcgcctc cgccgatttg cagtgcaccg
     1021 gacggaacca gcccagccgc atcagctcgg cgatgccgcg cgcgtctctg cgatcggtct
     1081 tcaccggcat cgtcttgaag gcgttgcgaa cgtgacgggt ctccagcagt tcgaccgcaa
     1141 gaccagcttg cgtcatcgcc gcgaaaagcc actgcgacag tggcccagct tccagtccga
     1201 tccgctccag gccgaagccg agcccaccga accaggcaat cagcgcatcc ggctcgctca
     1261 atacctttcc ctcccgcacg atcttgccgc tgccgtccaa aacacacacg ctcgagtatt
     1321 ccaatgacac gtcgattcct gcatagtagt tcatggtcgt ccctcgatga tgcttggagc
     1381 gggctcgccc gactccgttg aattgacaca ccatcagttt gagggacgac cgcctacttt
     1441 gccaagcaag ccgcacgcga agcgcgccat caaatagcgc cgtagcgagc gggcggcttg
     1501 cttggctcgg cttgctcggt gccggccggc ccgttacccc ctctaccggc ccggcgcgac
     1561 ggtgccccgg ggatgattat gtcacgcaga gcataatccg tcgaaccaat cagcaggttg
     1621 caagcacgga tcgagcaggg ggtgaacgaa ttgtctgaag cagatatcca gctgctgctg
     1681 accgcgctcg gcggggtggt gttgctggtg gcgctcatcg ttagccgcat tcggctgcat
     1741 cccttgctcg cc
//
LOCUS       DASWZT010000305        14636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_70094, whole genome shotgun sequence.
ACCESSION   DASWZT010000305 DASWZT010000000
VERSION     DASWZT010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14636
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70094"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VGE92_08255"
     CDS             <1..264
                     /locus_tag="VGE92_08255"
                     /inference="COORDINATES: protein motif:HMM:NF016262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HEY1313855.1"
                     /translation="TMSDSLLEFPADVPIKVMGRHDSDLRTLTQSIIERHAGPQPESS
                     VRTRTSADGNFVALTYLVHAQSRAQLDTIYRELTACKAVLMAL"
     gene            complement(219..896)
                     /gene="lipB"
                     /locus_tag="VGE92_08260"
     CDS             complement(219..896)
                     /gene="lipB"
                     /locus_tag="VGE92_08260"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010922.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEY1313856.1"
                     /translation="MSDAPLIRHLGLAPYEPTWRAMQEFTGQRASSTADEIWFVEHPP
                     VFTLGLNASREHLLSPGDIPVVQIDRGGQVTYHGPGQLVMYPLIDLRRRRLNVRELVV
                     ALENAVIDCVAEFGVSAQGSRAAPGVYVGGAKLASLGLRIRRGSSYHGLALNVSLDLA
                     PFARINVCGQRGLAVTRLADHVPRFAADGALEAAADALTPHLLRQLKFGAGYSAINTA
                     LQAVSSR"
     gene            complement(893..1930)
                     /gene="lipA"
                     /locus_tag="VGE92_08265"
     CDS             complement(893..1930)
                     /gene="lipA"
                     /locus_tag="VGE92_08265"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010331747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEY1313857.1"
                     /translation="MSEFKGIPILQEPRAAKSGEKYTTRQGFSAIKDGVRPKPAPPGG
                     APALRKPAWLRAPMGIGREFTRVKQIVREHRLSTVCEEAKCPNIGECWNAGTATIMLM
                     GAVCTRACRFCAVDTGNPRGWLDPEEPQNVARSVALMGLKYVVLTSVNRDDLDDGGAA
                     HYAAAIRAIKRSNPATAVEALTPDFQGVIRDVHTVLDSGLDVFAQNIETVRRLTHPVR
                     DPRAGYEQTLGVLRAAKAYRPAVLTKSSLMLGLGESEPELRETLAQLREAAVDILTLG
                     QYLRPTPNHLAVERFVTPREFDQYREWALALGFLECVSGPLVRSSYRAEQALARNNAG
                     LSNAALDSRAP"
     gene            complement(1945..2622)
                     /locus_tag="VGE92_08270"
     CDS             complement(1945..2622)
                     /locus_tag="VGE92_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY1313858.1"
                     /translation="MSATLERPRVAMILAAGRGERLRPLTDTTPKPLLKVRGQPLIER
                     HVVGLASAGMRKIVINLAWLGSQISEFLGDGARYGVTILYSHEERALETAGGIFRALP
                     HLTPEPFAVVNGDIYTDFPFDRLAMSAAREAHLVLVPNPPQHPQGDFGLEHGRPLAAA
                     ARRLTFSGIALYRHGFFAGCSDGAFPLKPLLLRSMAANQASAELYSGMWEDVGTVERL
                     QTLNANH"
     gene            complement(2619..3650)
                     /locus_tag="VGE92_08275"
     CDS             complement(2619..3650)
                     /locus_tag="VGE92_08275"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY1313859.1"
                     /translation="MGLPDASDSRLADLTRWVFEDLGFAGSAIAPASVDASFRRYFRV
                     TRGSDSYIAMDAPPDKENVEPFVRVARLLLGMGLNVPVILAQDARRGFLLLSDLGTRQ
                     YLDELKNGAEERLYADALGALARMQTAGAASARELAPYSRDLLLREMELMPQWFLREH
                     LKLDISAAERGMLDRLFETLAHSALAQPATFVHRDYHSRNLLLTATENPGILDFQDAV
                     CGAVTYDLVSLLKDCYIAWPAPRVRAWALLYRERLLAGGFAPAVSESEFIRGFDLMGL
                     QRHIKVLGIFARLNLRDGKPQYLKDLPRVLDYTREAAGEYAETAQFAEFIARRVDAEF
                     IYAQQRALT"
     gene            3722..6121
                     /gene="lptD"
                     /locus_tag="VGE92_08280"
     CDS             3722..6121
                     /gene="lptD"
                     /locus_tag="VGE92_08280"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEY1313860.1"
                     /translation="MAFDVRAAFIGLCLAAAAVVRAGDMCPAPPKYQPPLASDIAADD
                     HRIHIDTDDAVLDADGHAVLNGRVKVRQDARSVTSDSVDYDDSTGRVVVKGAVDFEDP
                     KLRVHSVTGSYDIDGAANFDQANFQLMDRNGRGFAKDIDVLPDGKVELASVRYTTCPV
                     GNEDWMLQASAINIDTKVQEGVARNVIMRFKDVPIFYTPYISFPIGDERKSGLLFPSF
                     GHSGSNGFELEVPYYFNLAPNYDLTLTPGLLSARGVQLAEQFRYLTSSSHGQFEGTFL
                     PDDSQVHSDRSYFRFTDITDFRTGMRFDTDIASVSDSSYFEDFAVGSDQTSVTFLERR
                     ADILYYDDSWRIRGQLQNFQVIDITVPDTERPYSRVPRIEANALWPVAGTQFELALDS
                     EAVNFLREVGTTGVRLNVAPELRWSSRGPGYFFEPAVGYDFTQYDLKNAAIGAPSTPT
                     RALPYARLDAGLVFERDAGSKGQRTQTLEPRVVYSYVPYRNQDSLPVFDSGLPDLNLT
                     ELFRTNRYVGEDRIGDANQVALALTTRLIDHSSGAQYLSATIGQIRYFSVPRVGLPAD
                     VAADATGQILQTVPGVNPLAMPGQVLLNSRGQIVMSLPGQYVSGLPATFAAAALGQTT
                     PVFPASDIVADVAVTAYKHFSFNFDYLWNPYTSQTDKSEVSLQYRPDPSRVINIGYRF
                     QENVLTQQPILKQWDGSFAWPIADHWNTVGRWVYSLQDHQTIEQVAGFEYKSCCWKIQ
                     LVQRRYVRVTNNTPGLDTSIALQLELTGLSSVGKPANTFLEREIRGYSTRDPNAQNPA
                     P"
     gene            6142..7452
                     /locus_tag="VGE92_08285"
     CDS             6142..7452
                     /locus_tag="VGE92_08285"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY1313861.1"
                     /translation="MRKLAFFGLSALVAASLLSTLSYSQTRDIGVHGEMLDRIAAIVN
                     DGLVLKSELDAQMDAVTKRLQEQKVELPSQSVLKQQVLDRLILQEIQSQHAKRVGLTV
                     SDEQLNSALQEIASRNKIPFDQLPTALAAQGVDYKQYREGMRKELTLSTLRQRDVIAH
                     INVSPHELEQFLARQQTAAANDEFNVSHILLSLPEAATPQQLDEITHKAQDLAARASK
                     GEDFGQLAIANSNSQTALDGGQLGWRKGTQLPQFILDLVTHMKPGQVSEPVRTPSGFH
                     IVKLNERRSGEAQVIINQIHVRHILMKTNELDDDETVRQKLSKLRERILKGEDFAGLA
                     STNSLDPGSAPDGGDLGWSGPGTFVPEFDKAIADLKVNEISEPFKTRYGWHIVQMLGT
                     RTYDSTDDVRRQHAFAAIRESKADEETELWLRRLRDEAFVEIKM"
     gene            7452..8450
                     /gene="pdxA"
                     /locus_tag="VGE92_08290"
     CDS             7452..8450
                     /gene="pdxA"
                     /locus_tag="VGE92_08290"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015572650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY1313862.1"
                     /translation="MYIPRIIITSGEPAGIGPDACVALAQGSWDADIVVAADANLLAA
                     TAAALHLPLNVERYDPSNPPAAHRAGTLKVLHLPTACAVTAGTLDPRNAAYVIEMLNR
                     ACDGCMNGEFAAMVTAPVQKSTLMDAGYEFTGHTEYLAARTRAALPVMLLLSGQLRVA
                     LVTTHLPLAEVPRALTRERLHSTLKIVAMDLERHFGLANPRIAVLGLNPHAGEAGHLG
                     REEIEVIEPVIRTLRAQGLDLQGPVPADTAFTPRFLESADVIVAMYHDQGLPVIKHVG
                     FGNAVNVTLGLPILRTSVDHGTALGLARTGRADAGSLRAALILAVELSGGSAHAGS"
     gene            8437..9225
                     /gene="rsmA"
                     /locus_tag="VGE92_08295"
     CDS             8437..9225
                     /gene="rsmA"
                     /locus_tag="VGE92_08295"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEY1313863.1"
                     /translation="MPVRRRFGQHFLHAPGVIRRIIDAVAPRPGERVVEIGPGRGALT
                     WGLLERAGRLDVIEIDRDLAQALKADPRADNRLRVHVENVLDTDFIRLRGTGAPLRVV
                     GNLPYNISTPLLFRLLTQQAAICDMFFMLQKEVVDRMTAQPGTKDYGRLTVMLAAVAE
                     VQALFDVGPGAFQPRPKVWSAVVRLQPSTHPRFELGRDGVLRTLVTAAFSQRRKTLRN
                     SLKGLLSSQDIESCGIDPQLRPETLAPSQFGLLAAHYSRLVGYT"
     gene            9232..9663
                     /locus_tag="VGE92_08300"
     CDS             9232..9663
                     /locus_tag="VGE92_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY1313864.1"
                     /translation="MARYKKLLVLLDLSEDSEQIAIAGRDLAQDSNAAMVFLHVVEFV
                     PTEPLGETMLPTVQFEDELIERSRERLADLIKRLGYARTTGRVESGNTKAEILRVADQ
                     EKADLIVLGSRERHGLAFMVNFTEDTVLHAAHCDMLAVRLK"
     gene            9677..10063
                     /gene="apaG"
                     /locus_tag="VGE92_08305"
     CDS             9677..10063
                     /gene="apaG"
                     /locus_tag="VGE92_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEY1313865.1"
                     /translation="MTAAPVNQRIRVEVETAYIEEQSDPRDKRFVFSYTITIRNEGEV
                     PARLLTRHWIITDANGNVKETRGDGVVGEQPYLKPGQGFRYSSGAVIETPVGTMQGSY
                     QMVADDGQQFDAPIAPFRLAMPGVLH"
     gene            10078..10917
                     /locus_tag="VGE92_08310"
     CDS             10078..10917
                     /locus_tag="VGE92_08310"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001204.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEY1313866.1"
                     /translation="MALYAIGDVQGCDAELGALLAALRYSPDRDRLWFVGDLVNRGPE
                     SLAVLRRIHALGEAASVTLGNHDLHLLAVAFGAAQIRGGDTLSDILTAPDRDALLEWL
                     ASRPLFQEERALKLGMLHAGLAPQWDLPLARSCAREFEHALQHNPEKLFKRLYGDQPD
                     RWDPALEGEERLRFIVNCFTRLRYVDPDGRLMLRAKGSPKGSPKGSPKRALIPWFDAA
                     AARWHGSRLIFGHWSTLGFFDNGAVAGLDTGCVWGGALTALRLDDPESKPVQVPCAGA
                     AEP"
     gene            10955..11473
                     /locus_tag="VGE92_08315"
     CDS             10955..11473
                     /locus_tag="VGE92_08315"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF009763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HEY1313867.1"
                     /translation="MRLCPPFNLHGWIEQNRGLLKPPVGNKLLFQDSAFIVMAVGGPN
                     SRKDFHHDPSEEFFYQVEGDMVLKTVQEGRLTDIPIREGEVFLLPAEVPHSPQRPAGS
                     VGVVVERRRDPDELDGFSWYCENCGHRLYLERVAVRNIETQLPEIFARFYSSEMHRSC
                     TVCGTVMQAPGT"
     gene            11470..12735
                     /gene="kynU"
                     /locus_tag="VGE92_08320"
     CDS             11470..12735
                     /gene="kynU"
                     /locus_tag="VGE92_08320"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HEY1313868.1"
                     /translation="MTRDAAAAVALDANDPLAHFADQFHHPYDAGRKSVYLCGHSLGL
                     QPKAAAEIVQAELEDWRRLGVSGHHVARRPWIRYHEAASAPLAALVGAHESEVVAMNS
                     LTVNLHLMMVSFFRPDARRNRVLIEKSAFPSDRYAIVSQLKFHGLNAAQHLLEVEPRV
                     GERRLRTEDVIQRIEQEGAALALVLLPSVQYLSGQCLELEPMIAAARRVGACVGLDLA
                     HGIGNTLSSLHDWNVDFAVWCSYKYLNAGPGAIGGCFVHERHARSTDLPRFAGWWGHD
                     AAQRFRMGPDFDPIAGAQGWQISNPPVLSAAPLCASLAIFESAGLGALRAKSIALTGY
                     LERLLQTLPPGLVDIVTPAAAQERGCQLSLRLARSPAAAKRCHERLTAAGVITDWREP
                     DVVRMTPIPLYNSFTDVFAAVEALAQALA"
     gene            12765..14138
                     /locus_tag="VGE92_08325"
     CDS             12765..14138
                     /locus_tag="VGE92_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY1313869.1"
                     /translation="MSDEPSTVRIVGAGPTGALLAILLQRRGHAVELFESRSDPRRAG
                     SESGRSINLALADRGIHALQAAGVFQELKAALLPMRGRLIHPREGGTSLQPYGLPNEL
                     IYSISRQRLNQTLLEVAARQPGVSIHFEHRFESADFAHATAWLRDLKQDRLISVPMRP
                     LLAADGAGSPLRRALSALHLIEARETDLEHGYKELSIPADSAGRYTLEADALHIWPRG
                     NYMLIALPNEDGSFTATLFLAKQGEVSFERLQEPAAIERFLASSFPDARALMPHCVEE
                     FRAHPVGFLGTVSAVPWHHLGVAALIGDAAHAIVPFHGQGMNCCFEDCIEFDACLARH
                     TADGHTAWAHLFAEFGALRKPNTDAIAAMALDNYLEMRERVVHPKFQLQQALSLELER
                     RFPQRFIPRYSMVMFHHEIPYLIALERGAIQSGLLSELTAGGVSSLGDIDFARAESEV
                     CARLAPL"
     gene            complement(14140..>14636)
                     /locus_tag="VGE92_08330"
     CDS             complement(14140..>14636)
                     /locus_tag="VGE92_08330"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEY1313870.1"
                     /translation="ALELVVAVSENDVIGRGNQLPWHLPADLKHFKALTIGRPVLMGR
                     KTFESIGKALPQRLNIVLSRAADFSPADCVVVHTLDAARSAAAGESALMVIGGAEIYR
                     QCLPFARRIHLTLVHAEIAAGDTLFAGWRGPQWRQVSCERHEADDKNAHAYSFVTLER
                     TAQL"
BASE COUNT         2635 a         4788 c         4604 g         2609 t
ORIGIN      
        1 actatgagcg acagcctgtt ggaatttccc gccgatgtcc cgatcaaggt catggggcgt
       61 catgacagcg acctgcgcac gctcacccag agcatcatcg agcgtcacgc gggcccccag
      121 cccgagtcca gtgtgcgcac acgcaccagc gccgacggca atttcgtcgc gctcacctac
      181 ctcgtgcatg cgcaaagccg cgcgcagctc gatacgatct atcgcgaact gacggcctgc
      241 aaagcggtat tgatggcgct gtaacccgca ccgaacttca gctgccgcaa tagatgcggc
      301 gtcaaggcgt ccgccgccgc ctcgagcgcg ccgtcggcgg cgaatcgggg aacatggtcc
      361 gcgagccgcg tcacggcaag gccgcgctgc ccgcagacgt tgattcgcgc gaacggtgcg
      421 agatcgagcg acacgttgag cgccagtccg tggtagctcg agccgcggcg aatgcgcagg
      481 ccgaggctag cgagtttcgc gccgccgaca tacacgccgg gcgccgcgcg cgaaccctgc
      541 gcgctcacac cgaactcggc gacgcagtcg atgacggcat tctccagcgc caccaccaac
      601 tcgcgcacgt tcaagcggcg gcgccgcaaa tcgatgagcg gatacatcac gagctgcccg
      661 ggcccgtggt aagtcacttg accgccgcga tcgatctgca ccaccgggat gtcgcccggg
      721 gataacagat gttcgcggct cgcattcaat cccaaggtga acacgggcgg atgctcgacg
      781 aaccagattt catcggcggt cgagctcgcg cgctggcccg tgaactcctg catcgcccgc
      841 caggtgggct cgtacggcgc caaaccgagg tgcctgatca gaggtgcatc gctcacgggg
      901 cgcggctgtc gagggcggcg ttgctgaggc cggcattgtt gcgcgccagc gcctgttccg
      961 cacggtagct cgatcgcacc agcggcccgg atacacattc gaggaatccc aaggcgagtg
     1021 cccactcgcg gtactgatcg aattcgcgcg gcgtgacgaa gcgctcgacg gcgaggtggt
     1081 tcggggtggg acgcaaatac tgcccgaggg tgagaatatc gaccgccgcc tcgcgcagct
     1141 gcgccaacgt ctcgcgaagc tccggctcgc tctcgcccag acccagcatc aaactgctct
     1201 tggtcagcac cgcgggccga tacgccttcg ccgcacgcag cacgcccaag gtctgctcgt
     1261 aaccggcgcg cgggtcgcgc accggatgcg tgaggcggcg caccgtctcg atattttgcg
     1321 cgaacacgtc caatcctgaa tcgaggacgg tgtgcacgtc gcggatcacg ccctgaaaat
     1381 ccggggtcag cgcttcgacc gcggtcgcgg gattgctgcg cttgatggcg cggatcgccg
     1441 cggcgtagtg cgccgcaccg ccgtcgtcga ggtcatcccg attgaccgat gtcagcacca
     1501 cgtacttgag ccccatcaat gcgaccgagc gcgccacgtt ctgcggctcc tccgggtcga
     1561 gccagccgcg cggattgccg gtgtcgaccg cgcagaatcg gcaggcgcgc gtgcacaccg
     1621 cgcccattaa cataatcgtc gccgttccgg cgttccagca ttcgccgata ttcgggcact
     1681 tcgcttcctc gcacaccgta ctcaagcgat gctcgcgtac gatttgcttg accctcgtga
     1741 attcgcggcc gatgcccatg ggcgcacgca gccaggcggg cttgcgcaag gccggcgcgc
     1801 cgccgggcgg cgccggcttc ggcctgacgc catccttgat ggcgctaaag ccctgccgag
     1861 tggtgtactt ctcgccgctc ttcgccgcgc gcggctcttg caggatgggt atgcctttga
     1921 attcggacat ggctcaattg taccttaatg gttggcgttc aacgtctgca gccgctcgac
     1981 ggttccaacg tcttcccaca tgccgctgta gagttccgcg gatgcctggt tcgccgccat
     2041 cgagcgcagc agcaacggct tcaacgggaa cgcaccgtcg ctgcagccgg cgaaaaatcc
     2101 atgccgatag agcgcgatgc cggagaacgt caagcggcgc gccgccgccg ccagcgggcg
     2161 tccgtgctcc aagccgaaat ccccttgcgg gtgttggggc ggattgggca cgagcacgag
     2221 gtgcgcttcg cgagcggcgc tcattgccag cctgtcgaat ggaaaatccg tatagatgtc
     2281 gccattgacc acggcaaacg gttccggtgt gagatgcggc aatgcgcgaa atataccgcc
     2341 ggcggtttcg agcgcgcgct cctcgtggct gtagaggatc gtcacaccat agcgtgcgcc
     2401 atcccccaag aattcgctga tctgcgaacc cagccacgcg agattgatca cgatcttgcg
     2461 catcccggcg cttgccaagc ctacgacgtg ccgttcgatc agcggctgac cgcgtacctt
     2521 gagcaatggc ttcggggtgg tgtcggtcaa cggccgcagg cgctcgccgc gccccgccgc
     2581 caggatcatg gcgacgcgcg gacgctcgag ggtcgcgctc acgtgagcgc ccgctgctgc
     2641 gcgtaaatga attccgcatc gacgcgcctg gcgatgaact cggcgaactg cgcggtttcc
     2701 gcatactcgc cggcggcctc gcgggtgtaa tcgagcacgc gcggcaagtc cttcaggtac
     2761 tgcggcttgc cgtcgcgcag gttcaagcgc gcgaagatcc cgagcacctt gatgtgccgc
     2821 tgcaaaccca tcagatcgaa gccgcggatg aattcgctct cggagacggc cggcgcgaat
     2881 ccgccggcga gcagccgttc gcgatatagc agcgcccagg cgcgcacccg cggcgcaggc
     2941 caggcgatgt agcaatcctt cagcagcgac accaaatcgt aggtcaccgc tccgcacacg
     3001 gcatcctgaa aatccaagat gccgggattc tcggtcgccg tgagcagcag attgcgtgaa
     3061 tgataatcgc gatgcacgaa ggtcgcgggc tgcgccaggg cactgtgcgc cagcgtctcg
     3121 aacagccgat cgagcatgcc gcgttccgcc gcggagatgt cgagcttgag gtgctcgcgc
     3181 aggaaccact gcggcatgag ttccatttca cgcagcaaca aatcgcggct gtacggcgcc
     3241 agctcgcgcg ccgacgccgc accggccgtc tgcatcctcg ccaaggcgcc gagcgcatcc
     3301 gcatacagcc gctcttccgc tccattcttg agctcgtcga ggtactggcg ggttcctaga
     3361 tcggacagca gcagaaagcc gcgccgtgca tcctgcgcca aaatcaccgg tacgttgagg
     3421 cccatgccga gcagcagccg cgccacgcgc acgaacggtt ccacgttctc cttgtccggc
     3481 ggcgcgtcca ttgcgatgta actgtcgctg ccgcgggtca cgcgaaaata acggcgaaaa
     3541 ctcgcatcga ccgacgccgg cgcgatggcg ctgccggcaa atcccaagtc ctcgaacacc
     3601 catcgagtca aatccgccag gcgggaatcg cttgcatccg gtagacccat gcttgaccat
     3661 tcactaaccg caaatctggc ggcggcaact agtataccga ctaaggcgat gtattttgtc
     3721 catggcgttt gatgttcggg ccgcattcat tggcttgtgc ctcgcggccg ctgcggtggt
     3781 ccgcgccgga gacatgtgtc ccgctccgcc gaaataccaa cccccgctcg cctccgacat
     3841 cgccgccgac gatcaccgca tccacatcga caccgacgat gccgtgctcg acgccgacgg
     3901 ccatgccgtg ctcaatggcc gcgtcaaggt gcgccaggac gcgcgcagcg tcacctcgga
     3961 ctcggtcgat tacgacgaca gcaccggccg cgtcgttgtc aagggcgcgg tcgacttcga
     4021 agatccgaaa ttgcgggtgc acagcgtcac cggcagctac gacatcgacg gcgccgcgaa
     4081 tttcgatcag gcgaattttc aactcatgga ccgcaacggc cgcggtttcg ccaaggacat
     4141 cgacgtcctc cccgacggca aagtcgagct ggcgagcgtg cgctatacca cctgcccggt
     4201 gggcaacgag gattggatgc tgcaggcctc cgcgatcaac atcgacacca aggtgcagga
     4261 aggcgtggcg cgcaacgtca tcatgcgctt caaggatgtg cccatcttct atacgccgta
     4321 catttcgttc ccgatcggcg atgaacgcaa gagcggcctg ctgttcccga gtttcggcca
     4381 ttctggaagc aacggcttcg agctcgaggt tccgtactat ttcaatcttg cgccgaacta
     4441 cgatctgacg ctcacgcccg gactgctgtc ggcgcgcggc gtgcagctcg ccgaacagtt
     4501 tcgctacctc acctcgagtt cgcacggcca gttcgaaggc acgttcttgc cggacgattc
     4561 gcaggtgcac agcgatcgca gctacttccg cttcaccgac atcaccgatt ttcgcaccgg
     4621 gatgcgcttc gataccgaca tcgccagtgt cagcgacagc agctatttcg aggactttgc
     4681 cgtcggctcc gaccaaacca gcgtgacctt cctcgaacgg cgcgccgata tcctgtacta
     4741 cgacgactcc tggcgcatcc gcggacagct gcagaacttt caagttatcg acatcacggt
     4801 gcccgacacg gagcgcccct actcgcgcgt gccgcgaatc gaagcgaacg cgctgtggcc
     4861 ggtggccggc acgcaattcg agctggcgtt ggacagcgag gcggtgaatt ttctgcgcga
     4921 ggtcggcacc acgggcgtgc gcttgaacgt ggcgccggag ctgcgctggt cgagccgcgg
     4981 tccgggttat ttcttcgaac ccgcggtcgg ctacgatttt acgcaatacg acttgaagaa
     5041 cgccgccatc ggcgcgccga gcacgccgac ccgtgcgcta ccctacgcgc ggctcgatgc
     5101 gggtctggtg ttcgagcgcg acgcaggttc gaaggggcag cggacgcaga cgctcgagcc
     5161 gcgcgtggtc tatagctacg taccgtaccg caaccaagat tcgctgccgg tcttcgacag
     5221 cggtttaccc gacctcaacc tgaccgagct atttcgcacc aatcgctatg tcggggagga
     5281 ccgcatcggc gatgccaacc aagtcgcgct cgctctgacc acgcggctca tcgaccactc
     5341 gtcgggggct cagtacctca gcgcaaccat cggccagatc cgctatttct ccgtgccgcg
     5401 tgtcgggctt cccgcggacg tcgccgccga cgcgaccggt cagatcctgc agaccgtgcc
     5461 cggggtcaat cctctggcca tgccgggcca ggtgctgctg aattcgcgtg gtcagatcgt
     5521 gatgtccctg cccggacagt acgtctcggg actgcccgcg acgttcgcgg cggcggcgct
     5581 cggccagacg acgcccgtgt ttcccgcctc cgacatcgtc gccgatgtgg ccgtgaccgc
     5641 ctacaagcat ttcagcttca atttcgatta cctgtggaat ccctacacct cgcagaccga
     5701 taaatcggag gtgtcgctgc agtatcgccc cgacccttcg cgggtcatca atatcggcta
     5761 ccgctttcag gaaaatgtgc tcacccagca gcccatcctc aagcagtggg atgggtcctt
     5821 cgcctggccg atcgccgacc attggaacac ggtgggacgc tgggtctact ccctccagga
     5881 ccaccaaacc atcgagcaag tggctggatt tgaatataaa agttgctgct ggaagattca
     5941 gttggtgcaa cgccgctacg tccgggtcac caataacacg ccgggactag acacctcgat
     6001 tgcattacaa ttggaactca caggcctaag ttcggtcgga aaaccagcaa ataccttctt
     6061 ggaacgagag attcgcggtt attcgacccg cgatccgaat gcgcagaacc cggccccctg
     6121 accaggacga atttttaaac catgcgtaaa ttggcttttt ttggcttgag cgcgttagtc
     6181 gcggcatcgc tcctctcgac cctgtcgtat tcgcagacgc gcgatatcgg cgtgcacggt
     6241 gaaatgctcg accgcatcgc cgccatcgtc aatgacggtc tggtgctgaa gagcgagctc
     6301 gatgcgcaga tggatgcggt cacgaaaagg ctgcaggaac aaaaggtcga actgccctcg
     6361 caaagcgtgc tcaagcaaca ggtgctcgac cgcctgatcc tgcaggagat tcaatcgcag
     6421 cacgccaagc gggtggggct taccgtctcc gacgagcaac tgaacagcgc cctgcaggaa
     6481 atcgccagcc gcaacaagat cccgttcgat cagctgccca cggcgctcgc ggcgcagggc
     6541 gtcgattaca agcagtatcg cgagggcatg cgcaaggaat tgaccctgag taccttgcgg
     6601 caacgcgacg tcattgcgca catcaatgtc agtccgcatg aactcgagca attcctggcg
     6661 cgccagcaga ccgccgccgc gaacgatgaa ttcaacgtgt cgcacattct gctgtcgctg
     6721 ccggaagccg cgacgccgca acagctcgat gaaatcaccc acaaggcaca ggatctggcg
     6781 gcgcgcgcct ccaagggaga ggacttcggt caactcgcga tcgccaattc gaacagccag
     6841 acggccctcg acggcggcca gctgggctgg cgcaaaggga cgcagctgcc gcagttcatc
     6901 ttggacctgg tcacgcacat gaagccgggt caagtgagcg agccggtgcg cacgccgagc
     6961 ggcttccaca tcgtcaaact caacgagcgg cgcagcggcg aagcgcaggt catcatcaat
     7021 cagattcacg tgcgccacat tttgatgaag accaacgaac tcgacgacga tgaaaccgtg
     7081 cgccagaaat tgagcaagct gcgcgagcgc atcttgaagg gcgaggattt tgccggcctc
     7141 gcgtccacca attcactgga tcccggttct gcgccggacg gcggcgacct cggctggagc
     7201 ggacccggga ccttcgttcc ggaattcgac aaggcgatcg ccgacttgaa ggtcaatgag
     7261 atcagcgagc cgttcaagac tcgttacggc tggcatatcg tgcagatgct gggcacccgc
     7321 acctacgaca gcaccgacga cgtccggcgg caacatgctt tcgcggccat ccgcgaaagc
     7381 aaggcggatg aggaaaccga gttgtggctg cgccgactac gcgacgaggc cttcgtcgaa
     7441 atcaagatgt agtgtacata ccgcggatca tcatcacgtc gggtgagccc gcgggcattg
     7501 gcccggatgc ctgcgtcgcg ctcgcacagg gcagctggga tgccgacatc gtggtggcgg
     7561 ccgatgcgaa tctactggcg gccacggccg ccgccctgca cctgccgctc aacgtcgagc
     7621 gctacgatcc ctcgaaccct cccgccgcgc atcgcgccgg cacgctcaag gtgttgcacc
     7681 tgcccacggc ctgcgccgtc accgccggga cgctcgatcc gcgcaatgcg gcctatgtga
     7741 tcgagatgct gaaccgtgcc tgcgatgggt gcatgaacgg cgagtttgcg gccatggtga
     7801 ccgcgcccgt gcagaagagc accttgatgg atgcggggta cgagttcacc ggccacaccg
     7861 aatacctcgc ggcccgcacc cgcgccgcgt tgccggtcat gctgttgctg agcggacagc
     7921 tgcgcgtggc actggtcacg acccacctgc cgctggccga ggtgccgcgg gcgctgacgc
     7981 gcgagcgcct gcacagcacc ttgaagatcg tggccatgga tctggagcgc catttcggac
     8041 tggcgaatcc gaggatcgcc gtgctcgggc tcaatccgca cgcgggcgaa gccggacatc
     8101 tcggccgcga ggaaatcgag gtgatcgagc ccgtgattcg cacgctgcgt gcgcaaggcc
     8161 tcgacctgca aggccccgtg ccggccgata ccgcctttac gccgcgtttc ctcgagtccg
     8221 ccgacgtcat cgtcgcgatg tatcacgatc aggggctgcc ggtgatcaag cacgtgggat
     8281 tcggcaacgc ggtcaacgtc accttgggac ttccgatcct gcgaaccagc gtcgaccacg
     8341 gaacggcgct cggcttggcg cgcaccggcc gggcggacgc cggcagcttg cgcgcggccc
     8401 tcatcctcgc cgtcgagttg agcggcggtt ccgcgcatgc cggttcgtag gcgcttcggt
     8461 cagcattttc tacatgctcc cggggtgatt cgccggatca tcgacgcagt cgcgccgcgg
     8521 ccgggcgagc gcgtcgtcga gatcggaccc ggccgcggcg cgctgacctg gggcctgctc
     8581 gaacgcgccg gccgcttgga tgtgatcgaa atcgatcgcg atctggcgca ggcactcaag
     8641 gccgatccgc gcgccgacaa ccgcctccgc gtgcatgtcg agaacgtgct cgacacggac
     8701 ttcatccgcc tgcgtggcac cggtgcgccg ctgcgcgtcg tcggcaacct cccctacaac
     8761 atctcgaccc cgctgctgtt tcgcctgctg acgcagcaag cagccatttg cgatatgttt
     8821 ttcatgctgc aaaaggaagt cgtggaccgg atgacggcgc agcccggcac caaggattat
     8881 gggcgcctga cggtgatgct ggctgcggtg gccgaggtgc aggcgctctt cgatgtcggc
     8941 cccggcgcgt ttcagccgcg ccccaaggta tggtcggccg tcgtgcgcct gcagccgagc
     9001 acacacccgc gcttcgagtt gggcagggat ggggtgctgc gaaccctggt gacggccgcg
     9061 ttctcgcagc ggcgcaaaac cctgcgcaac tccctgaagg gcctgctgag ttcacaggac
     9121 atcgaatcct gcggtattga cccgcagttg cgtccggaaa cgttggcacc gtcgcaattc
     9181 ggcctgcttg ccgcgcatta ctcgcgactg gtcggctata cttgatggcg catggcccga
     9241 tacaagaagc ttttggtgct gctcgatctg tccgaagaca gcgagcagat tgccattgcg
     9301 gggcgggatc tcgcgcagga ttcgaatgcg gccatggttt tcctgcatgt cgtcgaattc
     9361 gtgccgacgg aacccttggg cgaaacgatg ctgccgacgg tgcaattcga ggatgagctc
     9421 atcgagcgtt cgcgcgagag gctggccgac ctgatcaagc ggctggggta tgcgcgcacc
     9481 acggggcggg tcgaatccgg taataccaag gcggagattc tgcgcgtagc ggaccaggaa
     9541 aaggcggatc tcatcgtgct cggcagccgt gagcgtcacg gcctcgcttt catggtgaat
     9601 tttacggaag acacggtgct gcatgccgcg cattgcgaca tgctggccgt gcgcttgaag
     9661 taatgcgatg caaagcatga cagccgcgcc ggtgaaccag agaattcggg ttgaggtcga
     9721 gactgcctac atcgaggagc agtccgaccc gcgcgataag cgattcgtgt tttcgtacac
     9781 catcaccatc cgcaacgaag gtgaggtgcc ggcgcgcctg ctgacgcgcc actggatcat
     9841 taccgacgcg aacggcaatg tgaaagagac gcgcggcgac ggtgtggtcg gagaacagcc
     9901 gtatctgaag cccggccaag gatttcgcta ttcgagcggt gccgtcatcg aaactccggt
     9961 cggcaccatg cagggcagct atcaaatggt ggccgatgac ggacagcagt tcgatgcgcc
    10021 gatcgctcca ttcaggctgg cgatgcccgg ggtactgcat tagctcatga gcggctgatg
    10081 gcgctgtacg cgatcggtga cgtgcaaggg tgcgacgctg aactcggtgc cttgcttgcc
    10141 gcgctgcgct actcgccgga tcgggatcga ctgtggttcg tcggcgacct ggtcaatcgc
    10201 ggacccgaat ccctcgcggt gctgcgcaga atccatgcat tgggcgaagc ggcgagcgtc
    10261 accttgggca atcacgacct gcacctcttg gcggtggcgt ttggtgccgc ccaaatccgc
    10321 ggcggcgata ccttgagcga tatcctgacc gcgccggatc gcgacgccct cctcgagtgg
    10381 ctcgcgagcc ggcctctgtt tcaagaagaa cgtgcgctca agctgggtat gctgcacgcc
    10441 ggtttggcgc cgcaatggga cctgccgctg gcgcgaagtt gcgcgcgcga attcgagcat
    10501 gctctgcagc acaaccccga gaaactattc aagcggctgt acggcgatca accggatcgc
    10561 tgggaccctg ccttggaggg cgaggagcgt ttgcggttca tcgtgaattg cttcacgcgc
    10621 ctgcgctacg tcgacccgga cggccgcctc atgctgcgcg cgaaaggctc gccgaaaggc
    10681 tcgccgaaag gctcgccgaa aagggcgctg atcccgtggt tcgatgcagc ggcggcgcgc
    10741 tggcacgggt cgcggctcat cttcggccac tggtcgaccc tgggcttctt cgacaacggc
    10801 gcagtcgcgg gcctcgatac cggctgcgta tggggcggcg cgctgaccgc gctgcgcttg
    10861 gatgatcccg aatcgaagcc ggtgcaagtc ccctgcgcgg gcgccgcgga gccctagccg
    10921 cggtcgaggt tgcgcgccgc gctataatgc ggccatgcgc ctgtgtcctc ctttcaatct
    10981 gcacggctgg atcgagcaga atcgcggttt gctgaagccg cccgtcggca acaagctgct
    11041 gttccaagac agcgctttca tcgtcatggc cgtgggcggc cccaactcgc gcaaggattt
    11101 tcaccacgat ccgagcgaag agttcttcta ccaggtcgag ggcgacatgg tgctcaagac
    11161 ggtccaggag ggccgcctga ccgacattcc catccgcgag ggcgaggtgt ttctgttgcc
    11221 cgcggaagta ccgcattcgc cgcagcgccc ggccggcagc gtcggcgtcg tggtcgaacg
    11281 gcgccgcgat cccgacgagc tcgacggatt ctcctggtac tgcgagaact gcggccatcg
    11341 tctttacttg gaacgcgttg ccgtgcgcaa tattgaaacg cagttgccgg agatctttgc
    11401 gcgcttctac tcgagcgaga tgcatcgcag ctgcaccgtc tgcggcaccg tcatgcaggc
    11461 tccgggaacg tgacgcgcga cgcggccgcc gcggtggccc tggacgccaa cgatccgctc
    11521 gcgcatttcg cggatcaatt ccatcacccg tacgacgccg ggcgaaaatc agtgtatctg
    11581 tgcggccact cgctcggcct gcaacccaaa gcggcggccg agatcgtgca agccgaactc
    11641 gaggactggc ggcgcctggg cgtgtccgga catcatgtcg cgcgtcgccc ttggatccgc
    11701 tatcacgagg cggcgagcgc accgctggcc gctctggtcg gggcgcacga gagcgaagtg
    11761 gtggcgatga attcgctgac cgtgaatctg catttgatga tggtcagctt ttttcgtccc
    11821 gacgcgcgcc gcaatcgggt gctgatcgaa aagtccgcat tcccgtcgga tcgctatgcc
    11881 atcgtctcgc agcttaagtt tcacgggctg aacgcggcac agcatctgct cgaggtcgaa
    11941 ccgcgggtgg gcgagcgccg cttgagaacc gaagatgtca tccaacgcat cgagcaggag
    12001 ggagccgcgt tggcgctggt tctgttgccg agcgtgcagt acttgagcgg ccagtgcttg
    12061 gagctcgagc cgatgatcgc cgcggcgcgc cgcgtcggtg cctgcgtggg attggacctc
    12121 gcccacggca tcggcaacac gctgtcgagc ttgcatgact ggaatgtcga cttcgccgtg
    12181 tggtgcagct acaagtatct gaacgcaggt cccggcgcca tcggcggctg cttcgtgcac
    12241 gagcgacacg cacgcagcac cgacttgccg cgcttcgcgg gctggtgggg gcacgacgcg
    12301 gcgcagcgct ttcgaatggg accggatttc gaccccatcg ccggcgcgca gggctggcag
    12361 atcagcaatc caccggtcct ctccgcggcg cccttgtgcg catcgttggc aatctttgaa
    12421 agcgcgggct tgggcgcgct gcgcgccaag tcgatcgctc tcaccggcta cctcgagcgg
    12481 cttttgcaga ccttgccgcc gggcctggtg gatatcgtca caccagccgc agcgcaggag
    12541 cgtggttgtc aactgagcct gcgcttggcg cgttcacccg ccgcggccaa gcgctgccac
    12601 gagcggctga cggcggcggg cgtgatcacc gattggcgcg agcctgacgt ggtgcgcatg
    12661 acgcccatac cgctctacaa ttcattcacc gatgtgttcg ctgccgtcga ggccttggcg
    12721 caagccctgg cgtaagcctt ggcgaagcct tggcgtagcc ctccatgagt gacgagccca
    12781 gtaccgttcg catcgtcggc gcaggcccga ccggcgcact gctggccatt ttgttacagc
    12841 gccgcgggca tgccgtcgag ctgttcgaga gccgctccga cccgcgccgc gccggcagtg
    12901 aatccggccg atccatcaat ctcgcattgg cggatcgggg cattcatgca ttgcaagcag
    12961 ccggcgtgtt tcaggagctg aaagcggcgc tcctgccgat gcgcgggcgg ctcatccatc
    13021 cgcgcgaggg aggcacctcg ctgcagccgt atggcctgcc gaatgagctc atctactcga
    13081 tctcgcgcca gcggctgaac caaaccctcc tcgaggtcgc cgcgcgccag cccggcgtga
    13141 gcattcactt cgaacatcgc ttcgagtccg ccgatttcgc acacgccacc gcgtggctgc
    13201 gcgacctcaa gcaggatcgg ctgatcagcg tgcccatgcg gccgctcctg gctgccgacg
    13261 gcgccggctc gccgctgcgc cgcgccttga gcgcgctgca tctgatcgag gcgcgcgaga
    13321 ccgatctcga gcatggctac aaggagctca gcatcccggc ggattcggcc ggacgttaca
    13381 cgctcgaggc cgatgccttg cacatctggc cgcgcggcaa ttacatgttg attgcactgc
    13441 cgaacgagga tggcagcttt acggccactt tgttcctcgc caaacagggc gaggtcagct
    13501 tcgagcgctt gcaagagcct gcggccatcg agcgattcct ggcgtcaagc tttcccgatg
    13561 cccgcgcgtt gatgccgcac tgtgtcgagg agttcagggc tcatccggtc gggttcttgg
    13621 ggacggtgtc ggccgtccct tggcaccatc ttggggtcgc agcgctcatc ggcgatgcgg
    13681 cgcacgccat cgtgccgttt catggtcagg gaatgaactg ctgcttcgag gattgcatcg
    13741 agttcgacgc ctgtctggcg cggcacacgg cggatgggca tacggcgtgg gcgcatctgt
    13801 tcgccgagtt cggcgcgctg cgcaaaccga atacggatgc gatcgccgcc atggcgctcg
    13861 acaattattt ggagatgcgc gagcgcgtcg tgcatccgaa attccagctg caacaggcgt
    13921 tgtcgctcga gctcgaacgg cgttttccgc agcgcttcat tccgcgctat tccatggtga
    13981 tgttccacca cgagatcccg tatctgattg cactcgagcg cggcgccatc cagagcggac
    14041 tcttgtcgga attgacggcc ggcggcgtat cgagcctcgg cgacatcgat ttcgcgcgcg
    14101 cggaatccga agtttgcgcg cgcctcgcgc cgctgtgacc taaagttgcg ccgtccgctc
    14161 gagggtcacg aagctgtacg catgggcatt cttgtcatcc gcttcatggc gctcgcacga
    14221 gacctgccgc cactgcgggc cgcgccagcc ggcgaacagc gtgtcgcctg ccgcgatctc
    14281 ggcgtgcacc aacgtcaaat ggatgcgccg cgcaaacggc aaacactgcc gataaatctc
    14341 cgcaccgccg atcaccatga gcgcggattc tcctgccgcg gcgctgcgcg cggcgtcgag
    14401 cgtatgtacg accacgcagt cggccggcga aaaatccgcc gcacggctca gcacgatgtt
    14461 cagccgctgc ggcaacgcct tgccgatgga ctcgaaggtc ttgcggccca tcagcaccgg
    14521 ccggccgatg gtcagcgcct tgaaatgctt cagatccgcc ggcagatgcc agggcagctg
    14581 gttgccgcgg ccgatgacgt cattttcaga gaccgcgaca accagctcga gggcgg
//
LOCUS       DASWZT010000306         4306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_36182, whole genome shotgun sequence.
ACCESSION   DASWZT010000306 DASWZT010000000
VERSION     DASWZT010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36182"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="VGE92_08335"
     CDS             <1..1244
                     /locus_tag="VGE92_08335"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY1313871.1"
                     /translation="YWTYDATAGSRTAITIGELARSRVAALPRRLKRTLTHLDVIKAP
                     SAAFFGDDSGAVYALDAQKGTLLWKTQVDSHPLARIVGAPTLYNDRLYVAVSSTEDSA
                     AANPSYGCCTFRGSVVALEVAGGKISWKSYTVLEEPQPTRKNSVGIQEFGPAGAAVAS
                     SPTLDAKRNVLYVATGGSTTGIEQSLTDAVVAFDLTDGKLRWVKQLLRTDGVPAAPGS
                     LSSPVLRTLATGNQVLLAGKRSGIVYGLDPDHGGEILWQTKIAGGPAGASGGIAWGSA
                     ADHRNLYVAISGLLAQPANASGSLTALDMTTGAVRWHTASPQPPCSASDHGCSHGESQ
                     AVTVMPGSAFSGSLDGHLRAYSTIDGKILWDFDTAKDFQTKNGIKAGGGPLDNGGATI
                     VNGSVYVNSGNALLAFSVDGK"
     gene            1262..2803
                     /locus_tag="VGE92_08340"
     CDS             1262..2803
                     /locus_tag="VGE92_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY1313872.1"
                     /translation="MIFFKRLASAGLCLLGGCIGATAQGAAGHAADTQPAPAIENAVV
                     KIFATMRYPDPFKPWTKQAPSEATASGVVIEGKRILTNAHVVLYASQVQVQASAAGDK
                     LAATVVAVAPGIDLAVLQLDDPSFFDSHPPLARASKLPQIKDAVLAYGFPTGGNSLSI
                     TKGIVSRIEFVAYTYPVSGLRIQVDAAINPGNSGGPAIAGDKMIGLAFSKLGGDTQNI
                     AYIIPNEEVELFLKDIADGHYDGKPAMYDEVQTLENPALRDYLKLDKQVEGMVVHRPD
                     KTDAGYPLKEWDVLTRIGDTPIDNQGMVKLEGNLRVSFSYMIQRLANNGTVPMTLIRG
                     GKTLQIQLPVSAQHPTLVSDLRGSYPSYFIYGPLVFSTATWQLVSSFESNAGLLRALG
                     AVKSPLITRALDAPDADTEELVVISSPFFPHKLANGYSNPAGSVVRSINGTHVRSLKH
                     LVALLRDLKDPFVTIEFDQKGNEALVFSRTAMLAATDDILTDNGVRAQGSPNMMAVWQ
                     DKASN"
     gene            complement(2824..3435)
                     /locus_tag="VGE92_08345"
     CDS             complement(2824..3435)
                     /locus_tag="VGE92_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313873.1"
                     /translation="MAKKFSQRAEDLRRALAQEAARLMAEHGIEDFLQAKRKAADRLG
                     VNDVAVLPKNIEIVAALREHQRLFGRESHDHTLKEQRRIALHTMRMLDEFQPRLVGSV
                     LTGTATNYSDINLHLFADASESVAIRLLEIGVPHEFYERRVKMDAERSVNYPALRFEA
                     NGRTVDATVFPIDGIRQSPYSPVDGRPMKRADAREVSELVGPV"
     gene            complement(3440..>4306)
                     /locus_tag="VGE92_08350"
     CDS             complement(3440..>4306)
                     /locus_tag="VGE92_08350"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY1313874.1"
                     /translation="VSVRLMRAIGGDYVRNYVTRFGFDKSQLPNDLTLALGTAELSPL
                     QLAIGYSTFANGGYKVSAYSVDRIEDAGGKVLQQATPAVACFECNRVNDPAAPHAAAS
                     AGAARLQLDEAPHDGKTLISDKNLAPQIIRPQVAYLLADMMKDVIRRGTGVRARTLNR
                     DDIAGKTGTTNDAHDAWFNGFNGDLVTTVWIGFDQDRSLGEGEQGGRTAVPAWTYYMH
                     EALAGVPRHGVPVPDGIVTVRISPETGLLASADNPNGILEKFIEGNLPKSESYEGPNN
                     ANPMNDGDKPLF"
BASE COUNT          790 a         1401 c         1339 g          776 t
ORIGIN      
        1 cctattggac ctatgacgcc actgcgggca gccgcaccgc gattacgatc ggcgaactcg
       61 cgcgctcgcg cgtcgcagct ctgccgcggc gcctgaaacg cacattgacc cacctcgatg
      121 tcatcaaggc accgagcgcc gcattcttcg gcgacgacag cggtgccgtg tacgcgctcg
      181 acgcgcagaa aggcacgctg ctctggaaaa cccaagtgga cagccacccc ttggcgcgca
      241 tcgtgggcgc gccgacgctg tacaacgatc gtctgtacgt ggcggtatcc tcgaccgaag
      301 acagtgccgc cgccaatccg agctacggct gctgcacctt tcgcggcagc gtggtcgcgc
      361 tcgaggttgc cggcggcaaa atcagctgga agagctacac ggttctcgag gagccgcaac
      421 cgacccgcaa gaacagcgtc ggcattcaag aattcggccc ggccggcgcg gcggtggcct
      481 cgtcgccgac cctcgatgca aaacgcaatg tgctgtacgt cgcaaccggc ggctccacca
      541 ccgggattga gcagtctttg acggacgccg tggtcgcctt tgacctcacc gacggcaagc
      601 tgcgctgggt caagcagttg ctgcgcacgg acggcgtacc ggccgctccc ggctcgttga
      661 gttctcccgt actgcgcaca ctggcgaccg gcaaccaggt gctgctggca ggaaaaagat
      721 cggggatcgt gtacggtctg gatcccgatc acggtggcga gatcttgtgg cagacgaaga
      781 tcgcgggcgg ccctgccggc gcgtccggcg ggattgcctg gggcagcgcc gcggatcacc
      841 ggaatctgta tgtcgcgatc tcgggtctgc tggcgcagcc cgccaatgcc tccggcagtt
      901 tgaccgcgct cgatatgacc accggggcgg tgcgttggca cacggcgtcc ccgcagccgc
      961 cctgcagcgc gagcgatcac ggctgctcgc acggcgaatc gcaagccgtg acggtcatgc
     1021 cgggcagcgc attctccgga tcgctggacg ggcacctgcg cgcctattcg accatcgacg
     1081 gcaagattct ctgggacttc gacacggcca aggactttca aaccaagaac ggcatcaagg
     1141 ccggcggcgg acccctcgac aacggcggag cgacgatcgt gaacggcagc gtgtatgtca
     1201 attcgggtaa cgcgctgctc gccttttcgg tcgacggaaa ataaggaccc ttgcaatcac
     1261 catgatattt ttcaagcggc ttgcttcggc cggactgtgt ttgctcggtg gctgcatcgg
     1321 cgccacggcg cagggcgccg cggggcacgc cgccgatacg caacccgcgc cggcgatcga
     1381 aaacgcggtg gtgaaaatct tcgccacgat gcgctatccg gatcccttca aaccgtggac
     1441 gaaacaggcg ccgagcgagg ccaccgcctc gggtgtggtg atcgaaggca agcgcatcct
     1501 caccaatgcc catgtcgttc tgtacgcgag ccaggttcag gttcaagcga gtgccgcagg
     1561 cgacaaactg gccgccaccg tggtcgcggt cgccccgggc atcgatttgg ccgtgctgca
     1621 gctcgatgat cccagtttct tcgattcgca tccgccgctg gcgcgagcca gcaagctgcc
     1681 gcaaatcaag gacgccgttc tggcttacgg cttcccgacc ggcggcaatt cgctgtcgat
     1741 caccaaggga atcgtctcgc gcatcgaatt cgtcgcctac acctacccgg tgtcgggtct
     1801 gcgcatccag gtcgatgccg cgatcaatcc gggcaacagc ggcggcccgg caatcgccgg
     1861 cgacaagatg atcggtctcg cgttcagcaa actcgggggg gacacccaga acatcgccta
     1921 catcatcccc aacgaagagg tcgaattgtt cttgaaggac attgccgacg gccactacga
     1981 cggcaagccc gccatgtacg acgaagtgca gaccctcgag aacccggcgc tgcgcgacta
     2041 tctcaaactc gataagcaag tcgaagggat ggtcgttcac cgtcccgaca agaccgatgc
     2101 cggttacccg ctcaaggagt gggatgtcct gacacgcatc ggcgacacgc cgatcgacaa
     2161 tcagggaatg gtcaaactcg aggggaactt gcgggtcagc ttctcgtaca tgattcagcg
     2221 cctggccaac aacggcacgg tgccgatgac gctgatccgc ggcggcaaga ccctgcagat
     2281 tcaattgccg gtgtcggcgc agcaccccac cctggtgagc gacttgcgcg gcagctaccc
     2341 atcgtacttc atctacggtc cgctggtgtt ctcgaccgcg acgtggcaac tggtgtcgag
     2401 cttcgaaagc aacgccggat tgctgcgcgc tttgggcgcg gtgaagagcc cgctgatcac
     2461 gcgcgccctc gatgcccccg acgcggacac cgaagagctc gtcgtcatct cctcgccctt
     2521 cttcccgcac aagctcgcca acggctactc gaatcccgcg ggctcggtgg tgcgctccat
     2581 caacggcacc cacgtgcgca gcctcaagca cctcgtggcg ttgctgcgcg acctcaagga
     2641 tcccttcgtc accatcgagt tcgatcagaa gggcaacgaa gcgctggtgt tttcacgcac
     2701 cgccatgctc gccgccaccg acgacatcct gaccgacaac ggtgtgcgcg cgcagggctc
     2761 gccgaacatg atggcggtgt ggcaagacaa ggcatccaac tgacgcgcag gtggcgatcg
     2821 gattcagacc ggtcccacca gttccgacac ctcgcgtgca tcggcgcgct tcatgggccg
     2881 gccgtcgacc ggggagtagg gcgactgacg gatgccgtcg atcgggaaca cggtggcatc
     2941 gacggtgcgg ccgttcgcct cgaatcgcag cgccggatag ttgacgctgc gctccgcgtc
     3001 catcttgacg cggcgctcgt aaaactcgtg cggcacgcct atttcgagca gtcgaatcgc
     3061 caccgactcg gaagcatccg caaataaatg caggttgatg tcgctgtaat tggtggcggt
     3121 gccggtgagt accgagccca ccaaccgcgg ctggaactcg tccagcatgc gcatggtgtg
     3181 caaggcgatg cggcgctgtt ccttcaaggt atggtcgtgg gactcgcgcc cgaacaagcg
     3241 ctggtgttcg cgcagcgccg ctacgatttc gatattcttc ggcaatacgg cgacatcgtt
     3301 gacccccagg cgatcggctg ccttgcgctt cgcctgtagg aagtcctcga tgccgtgttc
     3361 ggccatgagg cgcgcggctt cctgggcgag agcgcggcgc aaatcctcgg cacgctgcga
     3421 aaacttctta gccatgttcc tagaatagcg gcttatcgcc gtcattcatc gggttcgcgt
     3481 tgttgggccc ctcgtagctc tccgatttgg ggaggtttcc ctcgatgaat ttctcgagga
     3541 tgccgttcgg gttgtccgcg ctcgccaaca gacccgtctc cggcgagata cgcaccgtga
     3601 cgatgccgtc gggcaccggc accccgtggc gaggcacgcc ggcgagcgcc tcgtgcatgt
     3661 agtaggtcca ggccggtacc gccgtacggc ctccctgctc gccctcgccg agcgagcgat
     3721 cctgatcgaa gccgatccag acggtggtca cgagatcgcc gttgaaaccg ttgaaccagg
     3781 catcgtgcgc atcgttggtc gtgccggttt tgccggcgat gtcgtcgcga ttcaaggtgc
     3841 gcgctcgaac cccggtgccg cgccgaatca catccttcat catgtcggcg agcagatagg
     3901 cgacttgcgg ccggatgatt tgcggtgcca aattcttatc tgaaatcaat gtcttaccgt
     3961 cgtgaggcgc ctcgtcgagt tggagccgag cggcgcctgc gctcgctgcg gcgtggggtg
     4021 ccgcgggatc gttgacccga ttacattcga aacaggccac ggcgggtgtc gcctgctgca
     4081 gcactttgcc gccggcgtct tcgatccggt cgacgctgta cgcgctgacc ttgtagccgc
     4141 cattggcgaa cgttgaatag ccgatcgcca gctgcaacgg actcaattcc gcggttccga
     4201 gcgcgagcgt cagatcattc ggcagctgcg atttatcgaa accgaagcgg gtgacgtaat
     4261 tgcgcacgta atcgccgccg atggcgcgca tcagccgcac cgacac
//
LOCUS       DASWZT010000307         5398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_55628, whole genome shotgun sequence.
ACCESSION   DASWZT010000307 DASWZT010000000
VERSION     DASWZT010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55628"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VGE92_08355"
     CDS             complement(<1..389)
                     /locus_tag="VGE92_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEY1313875.1"
                     /translation="MTGTATYDPMKQPRYTEIATFMRAPLSHNLAEVDIGLIGVPTDL
                     GVTNRAGARHGPREIRNSSSLMRTFNLALGVNPYELCRIADLGDVRLSHRFDLERQVE
                     EIAAFYREVHAAGVRPVSAGGDHSITYP"
     gene            complement(402..1793)
                     /locus_tag="VGE92_08360"
     CDS             complement(402..1793)
                     /locus_tag="VGE92_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1313876.1"
                     /translation="MTAAEIAARLDRLPPSRTIWSIVLLISAGCVFEFYDLFFTAYVG
                     PGMIDSGLFSAQSLGIFASLKIIRVAGFGTFVFCTFAGLWFGVVVFGQAADRFGRKAV
                     FTWSLMWYSICTAIMACQRTGQWLNIWRLIAGVGFGVQLVTVDTYIAELVPRSLRGRA
                     FSINQFVSFCIVPVVALLAWLIVPRTPFGLDGWRWVVGFGSIGALLVWGLRAGIPESP
                     RWLASVNRGAEADLIVSRIEARVAAEYGRPLPPSAPAAATASGTATLADIFAARYRRR
                     TLMLCIFNAAQAIGFYGFNSWVPTLLIARGITVTHGLQYAFIIAIAQPFGPLIGSCFA
                     DKVERKHQIIGGLAAMSILMAAFALASSAVALILIGIAFTLAANVMSYAYHGYQAELF
                     PTRIRSRAVGFVYSWSRIAAAFSGLIVGSLLASGGVGAVAVFIGAALLVGIAMIGIFG
                     PSTNGKALEELTN"
     gene            complement(1818..3239)
                     /locus_tag="VGE92_08365"
     CDS             complement(1818..3239)
                     /locus_tag="VGE92_08365"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY1313877.1"
                     /translation="MKTHALSTRLALNVAMLFGGASLAQAPPPDDSAFRALYKQLVEI
                     NTTRSVGSCTQAAQAMRARLLAAGIPATDTQILAPPERPQDGALIAVLHGRDAAAKPI
                     LLLAHIDVVEAQREDWERDPFKLVEEQGWFYARGASDDKAMAAVFTDTLIRYQQEGFK
                     PRRDIKLALTCGEETPEIFNSVRWLTETQPAVLNALFALNEGAGGELDRDEKPVALQI
                     QAGEKVYQDFALEASDVGGHSSRPTKNNPIVRLSAGLVRLGAHNFPIALNQATRAYFE
                     AEAKLVSPASAADMRAVLNNPRDEAAAERLWAVNPGWNGSLRTTCVVTEITGGHAPNA
                     LPQHVRANVNCRILPGVPISEVQEEITQALSDVKISVATSGEAGMQAPPPPLSDRIMQ
                     PVRKVGSAIWPGVAVVPTMSTGATDGRFLNAAGVPTYGLSGMFHDAEGSHAHGLNERI
                     RVKSLLDGRRFLYEVVKLYANGA"
     gene            3331..4059
                     /locus_tag="VGE92_08370"
     CDS             3331..4059
                     /locus_tag="VGE92_08370"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY1313878.1"
                     /translation="MRYDLFYWPTIQGRGEFVRLALEEAGAAYRDVARESGRGAGIAA
                     LERMLEDRQLERPPFAAPVLKAGTLFIGQTAAILQFLGLRHGLAPRAAAGRLWVQQLQ
                     LTMADFVSEVHDTHHPIGAGFYYEDQKKEAQRRAADFLKNRVPKFLGYFERVLAHNPR
                     GEPYLAGAKLTYADLSMFQIMAGMHYAFPRAIAAAAPKYRRLALLQERVAERPRIAAY
                     LESPRRIAFNREGIFRHYPELDAR"
     gene            4124..5350
                     /locus_tag="VGE92_08375"
     CDS             4124..5350
                     /locus_tag="VGE92_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313879.1"
                     /translation="MTIAGHAASFDAEGRLLPWTSWNDALDREMNFYQRCPADHGYPR
                     FVLATFLDGEWTPLAERTDTIPATQNGMGILSYLKFHDLRGGRDLRFLQTARAMGDYL
                     VTESLTPNAGKYPAFTRSTGKRDQFPLAADCGSQSDRPYEIEPDKGGIAGYALLKLYE
                     ATGEAKYLQQALQNARVLAANQQAGDSSRSPWPFRVDHRSGEGRGPISGNMTYILRLF
                     DSLLAQGYGEFMAARQGLWDWINVYQIPDAVAEGALFAQFFEDHDTPTNRSAWAPLNL
                     ARYLLEKKHFLDADWSAASEALIAFVRANFTHEEAGITVCHEQDEDHQAWGGINSTYG
                     AVLALHANATGCAQWAGEARQALNFTLYSIDEFGRPRDLANNPAPGGWQEDAHTDVIH
                     NYVDALVAFPEWGDVR"
BASE COUNT         1016 a         1704 c         1735 g          943 t
ORIGIN      
        1 gggtaagtga tcgagtgatc gccgcccgcc gacacgggcc gcaccccggc ggcatgcacc
       61 tcgcggtaga acgccgcaat ttcctcgacc tgccgttcga gatcgaagcg atgcgagagc
      121 cgcacatcgc ctaggtccgc gatccggcaa agctcatagg gattgacacc gagcgccaga
      181 ttgaaggtgc gcatgaggct cgaggagttg cgtatttcgc gaggtccgtg gcgtgcaccc
      241 gcgcggttgg tgactccaag atccgtcggg acaccgatca agccgatgtc gacctcggcc
      301 aggttgtgcg acagcggcgc gcgcatgaag gtcgcgattt cggtatagcg cggttgtttc
      361 atcggatcgt aggtcgcagt tccggtcatg gttgccgcct gtcagttggt caattcttcg
      421 agcgctttgc cgttggtcga gggtccaaat ataccgatca tcgcgatacc cacgagcagg
      481 gcggcgccga tgaaaacggc caccgccccg acaccgccgc tcgccagcaa ggaaccgacg
      541 atcaggccgg agaaggcggc cgcgatgcgg ctccacgagt acacaaagcc gactgctcgc
      601 gaacggattc gagtggggaa gagctctgcc tggtagccgt gataggcgta agacatcaca
      661 ttcgcggcca gcgtgaaggc gatgccaatg aggatcagcg ccacggcgga acttgcgagc
      721 gcaaatgccg ccatcaaaat cgacatggcg gcgagtccgc cgatgatttg atgtttgcgc
      781 tcgactttgt cggcgaaaca gctgccgatg agcggcccga agggctgcgc gatggcgatg
      841 atgaacgcgt attgcagccc gtgcgtgacg gtgataccgc gcgcgatgag caaggtgggt
      901 acccaggagt tgaatccgta aaagccgatg gcctgtgcgg cgttgaagat gcacagcatg
      961 agcgtgcggc ggcggtagcg ggcggcgaat atgtcggcga gcgttgcagt gccgcttgcg
     1021 gttgcggcgg ccggcgcgga gggcggcaac gggcggccgt actccgcggc aaccctggct
     1081 tcgatgcggc tgacgatgag gtccgcttcc gcgccgcggt tgacactcgc gagccaacgc
     1141 gggctttccg ggatgccggc ccgcagcccc catacgagca gtgcgccgat cgaaccgaat
     1201 ccaaccaccc agcgccaacc atccagcccg aacggagtgc gcggtacgat gagccacgcg
     1261 agcaaggcaa ccaccggcac gatgcaaaac gagacgaact gattgatgga gaacgcccgg
     1321 ccgcgcaggc tgcgcggcac cagctccgcg atgtaagtgt cgaccgtgac gagctgcacg
     1381 ccgaagccga cgccggcgat gaggcgccag atattgagcc actgcccggt tcgctggcag
     1441 gccatgatgg ccgtgcatat cgagtaccac atcagcgacc aagtgaagac cgccttacgg
     1501 ccgaagcggt ccgccgcctg gccgaagacc accacgccga accacaggcc cgcaaaggtg
     1561 caaaacacaa aagtaccgaa gcctgcgacg cggatgatct tgagcgaggc gaaaatcccg
     1621 agcgattgcg cgctgaacaa gccgctatcg atcataccgg gacccacata ggccgtaaaa
     1681 aacaaatcgt agaactcgaa cacacaaccc gcggagatca ggagcacgat gctccaaatc
     1741 gtgcgcgacg gcggcagccg atcgagccga gccgcgattt cagccgcagt cacacgccac
     1801 gccaatcgcg ttgagggtca tgcgccgttc gcatagagct tcaccacttc gtacagaaag
     1861 cgccggccat cgagcaatga cttcactcga atgcgctcat tgagtccgtg cgcatgactt
     1921 ccctcggcgt cgtgaaacat ccccgagagt ccataggtgg gcacgcccgc ggcgttgagg
     1981 aagcggccgt ccgtcgcgcc ggtcgacatc gtgggtacga cggcaacccc cggccaaatt
     2041 gccgagccga ctttgcgcac cggctgcatg atgcgatcgg acaggggtgg aggcggcgct
     2101 tgcatcccgg cctcgccgct cgtcgcgacg ctgattttga catcgctcag cgcttgagtg
     2161 atttcttctt gcacctcgct gatcgggacc cccggaagga ttcggcagtt cacgttggca
     2221 cgcacatgct gcggaagcgc gttgggcgcg tgaccgccgg tgatttccgt caccacacag
     2281 gtcgtgcgca gcgaaccatt ccagccggga ttcaccgccc acagccgctc ggccgccgct
     2341 tcatcgcgcg gattgttcag caccgcgcgc atgtcggcgg cgcttgccgg cgataccaac
     2401 ttggcctccg cctcgaagta ggcgcgtgtc gcctgattca aggcaatcgg gaagttgtgc
     2461 gcgccgagtc ttaccaagcc ggcgctcagg cgcacgatcg gattgttctt ggtcgggcgc
     2521 gagctgtgcc cgccgacatc ggaggcttcg agagcgaagt cctgatagac cttctcaccg
     2581 gcctgaatct gcaatgcgac cggcttctcg tcgcgatcga gctccccgcc cgcgccttca
     2641 ttgagcgcga acaatgcgtt caacacggcc ggctgggtct cggtcagcca gcgcacgctg
     2701 ttgaaaatct ccggggtttc ctcgccgcag gtgagcgcca acttgatgtc gcggcgcggc
     2761 ttgaagccct cttgttggta gcggatcagc gtatcggtga acaccgccgc catcgccttg
     2821 tcgtcgctcg cgccgcgcgc atagaaccaa ccctgctctt cgaccagttt gaatgggtcg
     2881 cgctcccaat cctcgcgctg cgcttcgacc acatcgatat gcgcgagcag cagaatcggc
     2941 ttggcggccg cgtcgcgtcc gtgcagcacg gcgatcagtg cgccgtcctg cggacgctcc
     3001 ggcggcgcca aaatttgcgt gtcggtggcg ggaattcccg ccgcgagcag acgcgcgcgc
     3061 atcgcttgcg ctgcttgggt gcagctaccc accgagcggg tcgtgttgat ctcgaccagt
     3121 tgtttgtaca gcgcgcgaaa cgcgctgtcg tccggcggcg gcgcttgcgc gagactcgcg
     3181 ccgccgaata gcatggcaac gttcagcgcg agacgtgtcg atagtgcgtg agttttcata
     3241 ccttggctgc catgctcccc tgaagtcgag tgtagggaac tcatcggtca aactgcaatc
     3301 tttcgaaggg gacctatgcg aggttggacg atgcgctacg atcttttcta ttggccgacg
     3361 atacagggcc gcggcgagtt cgtcaggctg gccttggagg aggcgggcgc ggcttatcgg
     3421 gacgtggcgc gcgagtccgg ccgcggagcc ggtatcgcgg cgctcgagcg catgctcgag
     3481 gatcgccagc tcgagcggcc gccgttcgct gcgcccgttc tcaaggccgg gacgctcttc
     3541 attggccaaa cggcagctat tttgcagttc ttaggcttgc ggcacggcct cgctccgcga
     3601 gcggcggccg gccggctgtg ggtgcaacaa ttgcagctca cgatggcgga tttcgtcagc
     3661 gaagtacacg acacgcatca tcccatcggc gccggcttct attacgagga tcagaagaag
     3721 gaggcacagc gccgcgcggc ggactttctg aaaaaccgcg ttccgaaatt tctcggttac
     3781 ttcgagcgcg tgctcgcgca caatccgcgc ggcgaacctt atctcgccgg cgcgaaactg
     3841 acgtacgcgg acttgtcgat gttccaaatc atggcgggca tgcactatgc ctttccgcgg
     3901 gctatcgccg cggcggcgcc caagtatcgg cgcctcgcgc tgctgcagga acgcgttgcc
     3961 gagcggccgc ggatcgccgc ctatctcgag tcgccgcggc gcatcgcgtt caaccgcgag
     4021 ggaatattcc gacactatcc cgagctcgac gctcgctgag gttgcgtttg agcactgagc
     4081 cggtatcgtg cagggcgtga cagatcttca aagtcgagcc gcggtgacga tcgcagggca
     4141 tgcggcgagc ttcgacgccg agggccggct gctgccgtgg acctcgtgga acgatgccct
     4201 cgaccgggaa atgaatttct accagcgctg cccggcggat catggctacc cgcgcttcgt
     4261 ccttgccacc ttcctcgacg gcgagtggac tcccctggcg gagcgcaccg atacgattcc
     4321 agccacgcag aatggaatgg ggatcctgtc ctaccttaaa ttccatgatc tgcgcggtgg
     4381 acgggatctg cggtttctgc agaccgcacg cgccatgggc gactacctgg tgaccgaatc
     4441 gttgacgccg aatgcgggca agtatccggc tttcacgcgc tcgaccggca agcgcgatca
     4501 gttcccgctg gcggcggatt gcggctcgca gagcgatcgt ccttacgaga ttgaacccga
     4561 caagggcgga attgcggggt acgccttgct caagctgtat gaagcgaccg gcgaggccaa
     4621 gtatttgcag caggctttgc agaacgctcg agtgctcgcg gcgaatcagc aagccgggga
     4681 tagctcgcgc tcgccgtggc cgtttcgcgt cgaccatcgc agcggcgagg gacgcggccc
     4741 gatctcgggc aacatgacct acatcctgcg gttgttcgac tcgctgcttg cgcaaggcta
     4801 cggcgaattc atggccgcgc ggcaaggcct gtgggactgg atcaacgtat atcagatccc
     4861 ggatgccgtc gccgaggggg cgctgttcgc gcagttcttc gaggaccatg acacgccgac
     4921 caatcgctcc gcctgggcgc cgttgaacct cgcgcgctac ctgctcgaga aaaagcattt
     4981 cctcgacgca gattggagcg ctgcctccga ggcgctgatc gctttcgtgc gcgccaactt
     5041 cacgcatgaa gaagcgggca tcaccgtttg ccacgaacag gatgaagatc accaggcctg
     5101 gggcggcatc aattcgacct acggcgccgt gctcgcgctg cacgcaaacg ccaccggctg
     5161 cgcgcaatgg gcgggcgaag cgcgccaggc gctgaacttc acgctgtatt cgatcgacga
     5221 attcggccgg ccgcgcgatc tggccaacaa ccccgcgccg ggcggctggc aggaggacgc
     5281 ccataccgac gtgatccaca actatgtcga cgcgttggtc gcatttcccg agtggggaga
     5341 cgttcgttag ctcggccggc ccccgaaaag cctgtgtccg aggcgaaaca agggccag
//
LOCUS       DASWZT010000308         4548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_74929, whole genome shotgun sequence.
ACCESSION   DASWZT010000308 DASWZT010000000
VERSION     DASWZT010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74929"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..2292
                     /gene="malQ"
                     /locus_tag="VGE92_08380"
     CDS             118..2292
                     /gene="malQ"
                     /locus_tag="VGE92_08380"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEY1313880.1"
                     /translation="MDQTLIERMARLRGIGDAYYDYRGELKYFDLETKKALLRAMGCK
                     VDEPAILARELSEIEVARWRKFLPPVAAAHGPRIGVDVNVAARDFGSVLTWSVRLEDG
                     SRREGTMSTADCPEIWRGEVEGSWITRRRFELPLDLPPGYHELTARTATSEADRCLLI
                     ISPPQCFEPKPVAAGRRLWGIAVQLYTLRSRDNWGIGDFNDLKLLIRWVALHGAGFVG
                     LNPLHALAPADPDRSSPYSASSRHFLNVLYIAVAAVPEFADCAAAHARLADPAIARRL
                     QELRARDLVDYRGVAELKFEFLELLYRDFRDQHLALGSARARDFLKFVDAGGKPLQMH
                     ARFDALDRYFRATLGVASGWLSWPAQYRNVNGKAAARFADAHREEVQFYVYLQWLAHE
                     QLAAAQALARELGMPIGLYGDYAVGSNPSGSETWVDQLSYRMGAEIGAPPDPLALKGQ
                     GWGIPPQDPFVMQTQCLQGFVHLIRDNMRYYGALRLDHVMSLFRLWWVAAGSSPTQGA
                     YVHYPLEQLLTVLSLESSRSACLVVGEDLGVVPDEMRRAMPEYGLYHYKVLLFEKLAG
                     RFRRPDEYARHALATVTTHDMPTLRSYWEGRDIELRRRLNLYPSAEVEADVRREREHD
                     RQLLMAALAEQGLKPAAPATAFEPFTAELAHALHLYLARSATALAALQIEDLLGMIDP
                     VNVPGTDKEYPNWQRKVTLDIEELAARPDLAAQLEEIHRARG"
     gene            2362..3951
                     /locus_tag="VGE92_08385"
     CDS             2362..3951
                     /locus_tag="VGE92_08385"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEY1313881.1"
                     /translation="MLDPVSALHILGRAGYGASPSSLRELSDAGAAAWIETQLAPRPD
                     LDADCNERLRAARLRIKYPAGDGWTAMDAARPLATLDAPISTLWHLVDHKNPMHVLER
                     RRPRDEVIAATVLRAVHSRWQLREVMCGFWHDHFNVDAAGSEAIGVALPAYDRDVIRR
                     HCFGNFRELLESCATSAAMQYYLSNRSSRAGSANENYARELFELHTLGRAAYLNDRYD
                     RWREVPGAEHGKAAGYIDQDVYEAARAFTGWTIEDGTGIDAHRKLPQSGQFVYVENWH
                     DGYQKRVLATEFDAFAPALADGRQVLDLVAAHPATARFLCDKLCRRFFGEDAPAQVRE
                     RMVQVWSAHVESHDQIARVLRTLLLSREFAAANGATGRHAAAKVRRPLALAAAFARAM
                     EIDLVYTVELGNEISSAGQNLFGWPTPTGLPDSAAPFLSSQAMRHRWSMLLGLAENSW
                     GNGAIASPAAGSALTTQTATAALFTRQLGVVPQERVSAIVSGCGWPADEPLQANGPAD
                     APRRWARLAAYCAMTPEFQVS"
     gene            3960..>4548
                     /locus_tag="VGE92_08390"
     CDS             3960..>4548
                     /locus_tag="VGE92_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313882.1"
                     /translation="MALSRRSIMQAAALAAASGLVPLPGLGRLSFGAQSLEDRILVVL
                     HLRGGCDGLNLLSPASDAEFIAARNSDLRVLAEGDEAGHPIEHSQEAAVEFRLHAAAK
                     SLAELADAKHLAFIHAAGLTDTTRSHFVATDMIEHGVADAASLSRTSSGWLARAMDTL
                     PGSQAVRGVCTSSAPSGVWSGDSRAISVADLGNGLS"
BASE COUNT          726 a         1436 c         1554 g          832 t
ORIGIN      
        1 acgggccgtg gatccgccac ttcgatacgg ccctcgacgg cacagaatcg caaagcctcg
       61 gtcaggcgcg cggctaccgg ctcgagccaa gcagcgccgc cctgttggag tgctgagatg
      121 gatcaaacgc tgatcgagag aatggcgcgc ctgcgcggca tcggcgatgc ctattacgat
      181 tatcggggcg agctcaagta tttcgatctg gagacgaaga aggcgctgct gcgcgcgatg
      241 ggttgcaagg tggatgagcc tgcgatcctg gcgcgcgagt tgagcgagat cgaggtggcg
      301 cgatggcgca agttcctgcc gccggtggcg gcggctcacg gcccgcgcat cggcgtcgac
      361 gtcaacgtcg cggcacgcga tttcgggtcc gtgctcacct ggtcggtgcg cctcgaggat
      421 ggttcgcgcc gcgaaggcac gatgtcgacg gccgattgcc ccgagatttg gcggggtgaa
      481 gtcgaggggt cctggatcac ccgccggcgc ttcgagttgc cgctcgatct gccgccgggc
      541 taccacgaat tgacggccag gacggccacg agcgaggccg atcggtgcct gctgatcatc
      601 tcgccgccgc aatgtttcga gcccaagccg gtcgccgccg ggcgtcggct gtggggaatc
      661 gccgtgcagc tgtacacgct gcggtcgcgc gacaattggg gaatcgggga tttcaatgat
      721 ctcaagctgt tgattcgctg ggtggcgctg catggcgccg gcttcgtcgg cttgaatccc
      781 ctgcacgcgc ttgcgccggc ggatccggac aggtcgagtc cgtacagcgc ttcgagccgg
      841 catttcctca atgtgctgta catcgcggtc gccgcggtgc cggaattcgc cgactgcgcg
      901 gcggcgcacg cacgcctggc cgatccggcc attgcgcgtc gcttgcagga attgcgcgcc
      961 cgcgacttgg tcgactatcg cggcgtcgcc gagctcaaat ttgaatttct cgaattgttg
     1021 tatcgcgatt ttcgcgatca gcatctggca ttaggcagcg cgcgggcacg ggatttcctg
     1081 aagtttgtcg atgccggcgg caagccgctg cagatgcacg cccgcttcga cgccttggac
     1141 cgctatttcc gcgcgacctt gggggtggcc tccgggtggc tcagttggcc cgcgcaatac
     1201 cgcaacgtga acggcaaggc cgcggctcga tttgccgacg cgcatcgcga ggaggtgcag
     1261 ttctacgtgt acttgcaatg gctggcgcat gagcaactcg ctgccgcgca ggctctggcg
     1321 cgcgaacttg gcatgcccat cggcctgtat ggcgactatg ccgtgggcag caatccatcg
     1381 ggttccgaga cctgggtcga tcagctgagc tatcgcatgg gtgccgagat cggtgccccg
     1441 cccgatccgt tggccttgaa gggccagggt tggggcatac cgccgcagga tcctttcgtc
     1501 atgcagacgc agtgtctgca aggcttcgtg catctgatac gcgacaacat gcgctactac
     1561 ggcgcgctgc gcctggatca cgtcatgtcg ttgttccgtc tgtggtgggt tgcggccggt
     1621 tcgtcgccca cgcaaggggc ttatgtgcat tatccgctcg agcagctgct cacggtactg
     1681 tctttggaaa gctcgcgcag cgcctgcttg gtggtcggtg aggacctggg ggtggtgccg
     1741 gatgagatgc gccgggccat gcccgagtac gggctttatc attacaaggt gctgttgttc
     1801 gaaaaattgg ccggccgttt ccgtcgtccc gacgagtatg cgcggcacgc cttggccacg
     1861 gtcacgaccc acgacatgcc gaccttgcgc agctactggg aggggcgcga catcgagttg
     1921 cgccgccgcc tgaatttgta cccgagcgct gaagtcgaag ccgatgtgcg gcgcgagcgc
     1981 gaacacgacc ggcaattgtt gatggcggcg ctcgccgagc agggactcaa accggctgcg
     2041 ccggcaacgg cgttcgaacc gttcaccgcc gagctcgcac atgccctgca cctgtatctc
     2101 gcgcgttcgg cgaccgcgct cgcggcgctg caaatcgagg accttttggg catgatcgac
     2161 ccggtgaatg tgccgggtac ggataaggaa tatccgaatt ggcagcgtaa ggtgacgctc
     2221 gacatcgagg agcttgcggc gcgcccggac ctggcggcgc agcttgagga gattcaccgc
     2281 gcacgcggct aattgcgcct catatgacag attggtcatc tcgcgaacgg ctgccgtgcg
     2341 cccctgtctc aaggatgtat cgtgctcgat cccgtatccg ctttgcatat tctcgggcgc
     2401 gccggctacg gcgcgagccc gagttccctg cgggaacttt cggacgcagg cgctgccgcg
     2461 tggatcgaaa cacagctggc gccgcggccg gacctcgacg ccgattgcaa tgagcgcctg
     2521 cgcgcggcgc gtctgcgcat caagtacccg gcgggcgatg gctggacagc gatggatgcg
     2581 gcccgaccgc tggcgacgct cgatgctccg atctcgacgt tgtggcacct cgtggatcac
     2641 aaaaatccca tgcacgtgct ggaacgccgg cggccgcgcg acgaggtcat cgccgccacg
     2701 gtgctgcgcg ccgtgcacag ccgctggcag ttgcgcgagg tcatgtgcgg cttctggcac
     2761 gatcacttca atgtcgatgc agccggcagc gaggcgatcg gggtcgcgtt gcccgcctat
     2821 gaccgcgatg tgatcagacg gcactgcttc ggcaactttc gcgagttgct cgagtcctgc
     2881 gcgaccagcg cggcgatgca atactatctc tcgaatcgat cctcgcgtgc cggatccgcc
     2941 aacgagaact acgcgcgcga actgttcgaa ctgcacaccc tgggacgcgc tgcttacctc
     3001 aacgatcgct acgatcgctg gcgcgaagtg cccggcgcgg agcacggcaa agcggcgggc
     3061 tacatcgacc aagacgtcta tgaggcggcg cgcgccttta ccggctggac catcgaggat
     3121 ggcacgggca tcgatgcgca tcgcaagctg ccgcagagcg gacagttcgt gtacgtggaa
     3181 aactggcacg acggctatca gaagcgggtg ctggccaccg agttcgacgc ctttgcgccg
     3241 gcactggcgg atggccggca agtgctcgac ctggtcgcgg cgcacccggc aacggcgcgc
     3301 tttctgtgcg acaaactttg ccgccgtttt tttggcgagg atgcgccggc tcaagtccgc
     3361 gaacgaatgg tgcaggtgtg gagcgcacac gtcgaatcgc acgatcaaat cgcgcgcgtg
     3421 ttgcgcacct tgttgttgtc gcgcgaattc gctgcagcga acggcgcaac cggccgacac
     3481 gcggcggcga aagtccgcag gccgctcgcg ctcgcggcgg ccttcgcgcg cgccatggaa
     3541 atcgatctgg tgtacacggt cgagctcggc aatgaaattt cgagcgcggg tcagaatctg
     3601 ttcggctggc cgacgccgac cgggctgccc gacagcgcgg cgccgtttct cagctcgcag
     3661 gccatgcggc atcgttggtc gatgctgctc ggcctcgcgg agaacagctg gggcaacggc
     3721 gcgatcgcaa gtcccgcggc cggcagcgcg ctgaccacgc agaccgcaac cgccgccctg
     3781 tttacgcgcc agcttggggt ggtgccgcag gagcgcgtgt cggccatcgt gtccggctgc
     3841 ggatggcccg ccgatgagcc gctgcaggcg aacggcccgg ccgacgcgcc gcgccgctgg
     3901 gcgcgcttgg cggcctattg cgcgatgacg cccgagtttc aggtcagctg aggtcatcga
     3961 tggcactatc gcgccgctcc atcatgcaag ccgccgcgtt ggccgcggct tccggcctcg
     4021 tgccgctccc gggactcggc cggctgtcgt tcggggcgca gtccctggag gaccgaatcc
     4081 tggtcgtgtt gcatttgcgc ggcggctgcg atggcttgaa tctgctgtcg ccggccagcg
     4141 atgccgagtt cattgcagca cgtaattccg atttacgggt gctcgccgag ggcgacgagg
     4201 cgggacatcc catcgagcac tcgcaggaag ccgcggtgga atttcggctg cacgcggcgg
     4261 cgaagtcgct ggcggagctt gccgatgcca agcacctggc cttcatccat gcggccggcc
     4321 tgaccgatac cacgcgcagt cacttcgtcg ccaccgatat gatcgagcac ggggttgcgg
     4381 atgccgcctc cttgagtcgg acgtcgagcg gttggttggc gcgcgccatg gacacgctgc
     4441 ccggttcgca agccgtgcgc ggcgtgtgca cctcgagcgc gccgagcggg gtatggagcg
     4501 gcgactcgcg ggcgatctcg gtcgccgatc tcggcaacgg cctaagcc
//
LOCUS       DASWZT010000309         5613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_84602, whole genome shotgun sequence.
ACCESSION   DASWZT010000309 DASWZT010000000
VERSION     DASWZT010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84602"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3518
                     /locus_tag="VGE92_08395"
     CDS             <1..3518
                     /locus_tag="VGE92_08395"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HEY1313883.1"
                     /translation="RQRIEKVIREALSAFGMESQVQIAESNLARIYIVARTNPNDMGR
                     VDSDALERRIAAAVRSWLDGFRAALLSRLDEAYALQLFEKYAQAFPAAYTEDFPGESA
                     ALDVSFLEAMEKEPARLHLDIYRPEPRRKDKFFLKIFRGQDAIPISDLLPMLENMGLK
                     VIAERPYQLEFPGGRHAWIQDLELVMQSSVARFETLEREIKSAFTAVWTGRMDSDSFN
                     QLTLSAGIPWRIVIVLRAYCRYLLQTGLPFSQGYIAQVLVNNPPIARALADVFVTRFD
                     PQRGAAARQKTLARLDERIRAALEEVTRSDEDRILRAVWNALTATVRTNAYQSDRSGQ
                     FKEYLSFKIESQQLRELPLPKPLFEIFVFSPRMEGVHLRMGYVARGGIRWSDRREDFR
                     TEVLGLMKAQQVKNTVIVPVGAKGGFVVKRLPAEREAQQAEVIACYQTLIRGMLDITD
                     NIVDDKITGPPLVVRHDNDDAYLVVAADKGTATFSDIANAISEEYGFWLGDAFASGGS
                     AGYDHKKMAITARGAWECVKRHFREMGVDIQAQNFTVAGIGDMAGDVFGNGMLQSQHI
                     RLVAAFNHMHIFIDPQPDAARSLAERRRLFKLPRSSWEDYSRAAISKGGGVFSRAAKS
                     LTLSREAQVLLELPAQVTPNEAIKAILKSHVDLFWNGGIGTYVKSTFESHSDVGDRSN
                     DAVRIDARELNCKVIGEGGNLGVSQLGRIEFALRGGKLNTDFIDNSAGVNCSDVEVNL
                     KILLNGAVRAGEITRAGRDRLLVQMTDDVAALVLRNNYLQSQAISTSEVQSKERLSES
                     AFVIRALERSGDLNRSLEFLPTEEEIAERRKAGEGLTRPELAITLSYGKIWLYRALIR
                     SNVPEDPYLSTELARYFPAPIQKRFAARLKRHRLRREIIATAITNSLINRMGPVFPVR
                     AQDDTGADPAAIARAYSIAREVFAARYIWTQIEALDNRIPAAVQYTAMSQTTRLLRHM
                     TYWLLENRRADLDIERGVLRYASKVGELFHELSSVLAITELTRLRTRHAGLIQQHVPE
                     SLAARIASLDSLHCALDLVEVAMAARLAIGYAAKAYFDLGERIGLTWIKEQIESLAAD
                     GHWQAVARGTLRDNLYQLQRQITGAVLKCKGRDAGARVGTWIARHETPVDALKRVVVD
                     LRTGSPPDFATLSVALQAVRRLAQD"
     gene            3523..4014
                     /locus_tag="VGE92_08400"
     CDS             3523..4014
                     /locus_tag="VGE92_08400"
                     /inference="COORDINATES: protein motif:HMM:NF021049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HEY1313884.1"
                     /translation="MSASTADFGRDYLQNRITREFVLAAHIGIMVESADDAAVVLSAP
                     LTPNANYKGTAFGGSLYSVAVLAGWAWVTRYLAARGLPADAVIQESNMRFLRPVLGAL
                     RARAAAPAGNEIDKFRKMLQRAGRGRISLQVEIGNNQSAAALFHGVFAAAVRQQEAPC
                     LEN"
     gene            3999..4748
                     /gene="gpmA"
                     /locus_tag="VGE92_08405"
     CDS             3999..4748
                     /gene="gpmA"
                     /locus_tag="VGE92_08405"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF010713.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEY1313885.1"
                     /translation="MPGKLILVRHGQSTWNVENRFTGWHDVDLSDLGRQEAAQAGQEL
                     QREQIEPNIAFTSVLKRAIRTLWLILDVTDRMWLPVERSWRLNERHYGALQGLDKAQT
                     VAKHGDAQVKIWRRSYDIPPPPLTLDDPRHPRFDARYKDVDAALLPVAESLKDTLSRV
                     LPYWESRIVPELRAGKNVLIVAHGNSLRALVKMLDMMSESDIVEFNIPTGIPILYELD
                     DQLQSLGRRFLGDPQAIAAAQDAVRRQTEKR"
     gene            complement(4757..5074)
                     /locus_tag="VGE92_08410"
     CDS             complement(4757..5074)
                     /locus_tag="VGE92_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY1313886.1"
                     /translation="MARMIVVDRDGKEHDIEAKPGLKIMEILRELDYGVAAICGGLCS
                     CATCHVYVDESWAARLPKPQSDELELLRELPDFKEATSRLSCQVDFTAALSGLKVAIA
                     PDS"
     gene            complement(5138..>5613)
                     /locus_tag="VGE92_08415"
     CDS             complement(5138..>5613)
                     /locus_tag="VGE92_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY1313887.1"
                     /translation="YYSGGPKAAARVRHAPDLREAAAKHLAAVAALPGVAPVLCHSDL
                     HTLNLIDRGDSLLLLDWEYAHASDPLWDLAGWSANNDLDEDLKQSLLSNYTGRRPTRD
                     ERSRLALLGWLYDYVCWAWSELYLQDAARADAAPDGVAARAAQLAARLQASGRAD"
BASE COUNT         1067 a         1762 c         1769 g         1015 t
ORIGIN      
        1 tccgtcagcg catcgaaaag gtcattcgcg aggcgctcag tgccttcggc atggaatcgc
       61 aggtgcagat cgcggagtcg aatctggcgc gcatctacat cgtcgcgcgt accaacccga
      121 acgacatggg ccgcgtcgac agcgatgcgc tcgaacgacg catcgctgcg gcggtgcgtt
      181 cttggctgga cggcttcagg gcggcgttgc tgtcgcgcct cgacgaggcg tatgccttgc
      241 agctgttcga gaagtacgcc caggcgttcc ccgcagccta cacggaggac ttcccaggag
      301 agtccgccgc tctcgacgtc tcgttcctcg aggcgatgga gaaggaaccg gcgcgcttgc
      361 atctcgacat ctaccggccc gagccgcggc gcaaggacaa attcttttta aagattttcc
      421 gtggtcaaga tgccattccg atttccgatc ttctgcccat gctggagaac atgggcctca
      481 aagtaattgc cgagcggcct taccagctcg agttcccggg cggccggcac gcctggatcc
      541 aggatctgga actcgtgatg cagtcgtccg tggcccgttt cgagaccttg gagcgcgaaa
      601 tcaagagcgc cttcacggcc gtgtggaccg gacgtatgga cagcgacagc ttcaatcaat
      661 tgacgctgtc cgccggcatc ccgtggcgca tcgtaatcgt cctgcgcgcc tattgccgct
      721 acttgttgca aaccggcctg ccgttcagcc agggctatat cgcccaggtg ctggtgaaca
      781 atccgccgat cgcgcgcgcc ttggcggatg tattcgtcac ccgcttcgat ccgcaacgcg
      841 gcgcggcggc gcgccaaaag acactggcgc gcctcgatga acgcatccgc gcggcgctgg
      901 aagaggtgac gcgctccgac gaagatcgca tcttgcgtgc cgtgtggaat gcgctgaccg
      961 cgacggtgcg caccaacgcc tatcaatccg accgcagcgg gcagttcaag gaatatctgt
     1021 cctttaaaat cgaaagccag caattgcgcg aactgccgct gcccaagccg ctgttcgaga
     1081 ttttcgtgtt ctcgccgcgc atggagggtg tgcacttgcg catgggttat gtcgcacgcg
     1141 gcggcattcg ctggtcggac cggcgcgagg acttccgcac cgaagtgctc gggctgatga
     1201 aggcgcagca ggtaaagaac acggtcatcg tcccggtggg ggccaagggc ggcttcgtgg
     1261 tcaagcgtct gcccgcggag cgcgaggcgc agcaagcgga ggtcatcgcc tgctatcaaa
     1321 cgctgatccg cggcatgctg gacatcaccg acaatatcgt cgatgacaaa atcaccggcc
     1381 cgccgctggt ggtgcggcac gacaacgacg atgcctatct ggtggtggcc gccgacaaag
     1441 gcacggcgac gttttccgat atcgccaacg ccatctccga ggagtacggg ttctggctgg
     1501 gcgacgcctt tgcctccggc ggctcggcgg gctacgacca caagaagatg gccatcaccg
     1561 cgcgcggcgc gtgggaatgc gtgaagcggc actttcgcga gatgggcgtc gacattcaag
     1621 cgcagaattt taccgtcgcc ggcatcggcg acatggcagg cgacgtattc ggcaatggta
     1681 tgctgcaatc acagcatatc cggctggtcg ccgccttcaa tcacatgcat atattcatcg
     1741 atccgcagcc cgatgccgcg cgttcgttgg cggagcgccg gcgtctgttc aaattgccgc
     1801 gctccagttg ggaggattat tcacgcgccg caatctccaa aggcggcgga gtgttttcgc
     1861 gcgccgccaa gagcctgacg ctgtcgcgcg aggcgcaggt gcttttggag cttccggctc
     1921 aggtgacgcc gaacgaggcg atcaaggcga ttctgaaatc gcacgtcgat ttgttttgga
     1981 acggcgggat cggcacctac gtcaaatcaa ccttcgaaag tcacagcgat gtcggcgacc
     2041 gcagcaacga cgcggtgcgg atcgacgcgc gggaattgaa ttgcaaggtc atcggcgagg
     2101 gcggcaatct cggcgtctcg cagctcggca gaatcgagtt cgcgctgcgc ggcggcaagc
     2161 tcaataccga cttcatcgac aactccgccg gagtcaactg ctcggacgtc gaagtcaact
     2221 tgaaaattct cttgaacggc gcagtgcgcg ccggcgagat cactcgcgca ggacgcgatc
     2281 ggctgctggt gcagatgacc gatgacgtgg ctgccctggt acttcgcaac aactatctgc
     2341 agagccaagc aatcagcacc agcgaggttc aatcgaaaga gcgcttgagc gagagcgcct
     2401 tcgtcatccg cgccctggag cgctcgggcg acctgaaccg ctcgctcgaa ttcctgccga
     2461 cagaggagga aatcgcagag cgccgcaaag ccggcgaggg cttgacgcgg ccggaactcg
     2521 ccatcacgtt aagttacggc aaaatctggc tgtaccgggc gctcattcgc tccaatgtgc
     2581 cggaagatcc gtacctctcg accgaattgg cgcgctattt cccggcgccg atccaaaagc
     2641 gtttcgcggc ccgcttgaaa cggcaccgcc tgcgccgcga gatcatcgcc accgcgatca
     2701 ccaatagttt gatcaatcgc atgggaccgg tgttcccggt gcgtgcccag gacgatactg
     2761 gcgcggatcc ggcagccatt gcgcgcgcct acagcatcgc gcgcgaggtg ttcgccgccc
     2821 gctacatctg gacgcaaatc gaggcgctgg ataaccgcat cccggcggcg gtgcagtaca
     2881 cggccatgtc gcagaccacg cgtctgctgc ggcacatgac ctactggttg ctcgaaaatc
     2941 ggcgcgcgga cctcgacatc gaacgcggcg tgctgcgcta cgccagcaaa gtcggggaat
     3001 tattccacga attgagcagc gttctcgcca tcacggagct cacccggctg cgcacgcgcc
     3061 atgccggact catccagcaa cacgtcccgg aatccctcgc cgcccgcatc gcctccctgg
     3121 attccttgca ctgcgccttg gatttggtcg aagtggcgat ggcggcacgg ctcgcaatag
     3181 gctacgccgc caaggcctac ttcgatctcg gcgaacgcat cggactcacg tggatcaagg
     3241 agcaaatcga aagtttggcg gcggacgggc attggcaggc cgtggcgcgc ggcacgctgc
     3301 gcgacaacct gtaccaattg cagcgccaga taaccggcgc ggtattgaaa tgcaagggcc
     3361 gcgatgcggg cgcgcgggtc ggcacatgga ttgcgcggca cgagacgccg gtggacgcgc
     3421 tgaaacgggt ggtggtcgat ctgcgtaccg gctcaccgcc tgatttcgcc actctgtccg
     3481 ttgccctgca ggcggttcgg cggctggctc aggattgacg gtgtgagcgc gagcaccgcc
     3541 gacttcgggc gtgattactt gcagaatcgc atcacccgcg aattcgtgct cgcggcgcat
     3601 atcggcatca tggtcgagtc ggccgacgat gccgcggtgg tgctgagcgc gccgctgacg
     3661 ccgaatgcca attacaaggg tacggcgttc ggcggcagcc tgtattcggt ggcggtcttg
     3721 gccggctggg cttgggtgac gcgctatctg gccgcgcgcg gcctgccggc ggatgccgtg
     3781 atccaggaat cgaacatgcg cttcttgcgg ccggtgctag gtgcgttgcg cgcgcgcgca
     3841 gcggctccag cgggcaacga gatcgacaaa ttccgcaaaa tgctgcagcg tgccggtcgc
     3901 ggacgaatta gtctgcaggt ggaaataggc aacaaccaaa gcgcggccgc gctttttcac
     3961 ggcgtgtttg ctgcggcggt acgtcaacag gaggctccgt gcctggaaaa ctgattttgg
     4021 tccgtcatgg gcaaagtacc tggaacgtag agaatcgctt caccggctgg cacgacgtag
     4081 atctcagcga cctcggccgc caagaagcgg cgcaagcggg gcaagagctg cagcgcgaac
     4141 aaattgagcc gaatattgcg tttacttcgg tgctaaagcg cgcgatccgc acgctctggt
     4201 tgatactcga tgtcacggac cgcatgtggc tgccggtgga acgaagttgg cgcctcaacg
     4261 agcgccatta cggtgctttg cagggactcg acaaggcaca aacggtcgcc aagcacggcg
     4321 acgcgcaggt caaaatctgg cggcgcagct acgatattcc gccgccgccc ctgacccttg
     4381 acgatccgcg ccatccgcgc ttcgatgcgc gctacaaaga tgtcgatgcg gcattgctac
     4441 cggtcgcgga atcgctcaag gacacgctgt cgcgggtcct gccttactgg gagtcgcgca
     4501 tcgtgcccga gcttcgagcc ggcaagaacg tactcatcgt cgcgcacgga aactcgttgc
     4561 gcgcgttggt caaaatgctc gacatgatgt ccgagagcga catcgtcgaa ttcaacattc
     4621 cgaccggcat cccgatactc tatgaactcg acgatcagct gcagtctctc ggccgccgct
     4681 tcctcggcga cccgcaggcc attgccgcgg cgcaagacgc ggtacgccgg caaaccgaga
     4741 agcgttaact taagtgtcag gaatccggtg cgatcgcgac cttgaggccc gacaacgccg
     4801 cagtgaagtc gacctggcac gataggcgcg atgtcgcctc tttgaaatcg ggcagctccc
     4861 gcagcaattc gagttcgtcg ctttgcggtt tcggcaagcg cgcagcccag ctttcatcga
     4921 cataaacatg gcacgtggcg caggagcaga ggccgccgca gatggcggcc actccgtagt
     4981 cgagctcacg cagaatctcc ataatcttca gaccgggctt cgcctcgatg tcatgttctt
     5041 tgccgtcccg gtccactacg atcatccgcg ccattgagcc tctccgttga atatcccctc
     5101 gcggggttcg ccgctggtgc gcctagtgtg ccggaagtta atcggcgcga ccacttgcct
     5161 ggaggcgtgc cgcgagttgc gcggcgcgcg ccgcgacgcc gtccggcgcg gcatcggccc
     5221 gggcggcgtc ctgcaggtac aattcgctcc aggcccaaca gacgtagtcg tacagccagc
     5281 cgagcaacgc caggcgcgag cgctcatcgc gcgtcggccg ccggccggtg tagttcgaca
     5341 acagactctg tttcaggtcc tcgtccagat cattgttggc gctccagccc gccaggtccc
     5401 agagcgggtc cgacgcatgc gcatactccc aatcgagcag cagcagcgag tcaccgcggt
     5461 cgatcaaatt gagggtgtgc aagtcgctgt ggcacagcac cggagcgacg ccgggcagcg
     5521 ccgccactgc cgcgaggtgc ttggccgccg cctcgcgcag atccggggcg tgccgcacgc
     5581 gagcggccgc cttagggccg cccgaatagt aat
//
LOCUS       DASWZT010000310        11813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_12197, whole genome shotgun sequence.
ACCESSION   DASWZT010000310 DASWZT010000000
VERSION     DASWZT010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11813
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12197"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..767
                     /locus_tag="VGE92_08420"
     CDS             282..767
                     /locus_tag="VGE92_08420"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY1313888.1"
                     /translation="MAKTSSSPRSVVHATFRSERTYPVSAAQVFKALADPAAKAKWFT
                     GGDGYTLLAREMDVRPGGREHVKGRLQTGVVTTFDATYHDVVVDQRIVYSYEMHLDDR
                     KISVSLATLELKPQGSATRLVMTEQGAFLDGYDDAGSREHGSNYLLDALGESLHMPGT
                     V"
     gene            810..1463
                     /locus_tag="VGE92_08425"
     CDS             810..1463
                     /locus_tag="VGE92_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY1313889.1"
                     /translation="MSLTLYAHPFAAYCWKVLIALYENATPFRYRLVEDAAGWAELAT
                     LWPIKKFPVLRDGDATIVESSIIVEYLMHRYPGTARMIPVDDEAALAVRFMDRFFDNY
                     IMTPMQTLVADRMRTDTERDTKGVADARKMLDVAYGWLEEKLVPDSWACGDSFSLADC
                     SAAPSLFYADWVHPLGDRFPGVLAYRGRVLARYSVARVIDEARPFRKFFPQGAPDRD"
     gene            1697..2440
                     /locus_tag="VGE92_08430"
     CDS             1697..2440
                     /locus_tag="VGE92_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313890.1"
                     /translation="MLDVHAPHEAVHTWKDFFIHIATIVIGLLIAVGLEQTVEYFHHR
                     QQVAEIRRSLAEERRINEVIFTGACNEFRRYAPILLGSLQTLAYLRTHPGAPPSQWPG
                     RFSFYILTIHFQDSAWKTALQSPALAYMPRVEVGIYSDIYDRLAKVSEQSLAEFHAVI
                     RAKSFMLQLTDPAMFDSEQSMQAYARVSDVVTSLYLMGIGERNMARLYSDFRGPPTDL
                     ELYALIPPAPAKQDVDAVREIAAPMEREK"
     gene            2490..3725
                     /locus_tag="VGE92_08435"
     CDS             2490..3725
                     /locus_tag="VGE92_08435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEY1313891.1"
                     /translation="MGTAAKSPLTRRSLIFGLAALPAMAARAWADDVELPSRLFFKEP
                     PAVKAADQALNVMEFEPLARAALPPAHFGYIATGSDDDRTLIQNHEAYNHYQIRAHRF
                     SDLSRLRTTRSVFGAQWPSPIYLSAVSAMRAFHPDGELAVARAARSRSMQLMLSTGSS
                     TPVEAVAAAAAAPLWQQLYPTDDWAVTTGIVRHAERAGCTAIVLTVDSHGVRNNETLK
                     RAMLSDSRTCSACHPGNSHDMWRRGPMFADLDVSRVTALAPAALTVAFLDRLRAVVKG
                     RLIVKGVVTGEDAALAVEHGADAIVISNHGGRNEETLRATVDCLPEVVAAVRARVPVF
                     LDGGIRRGTDIFKALALGATAVGIGRPQAWGLAAFGQPGVEAVIDILNRELAAIMRQA
                     GTPSIEDIVGADVIPTMRA"
     gene            3881..4132
                     /locus_tag="VGE92_08440"
     CDS             3881..4132
                     /locus_tag="VGE92_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313892.1"
                     /translation="MTSPMTVKRVLVAQLFGLVLLVAANQAGAKGCVKGAVVGAVAGH
                     VAGHHAVVGAAVGCAAGHHLAKKKQEEQRQQQPAPGQQT"
     gene            complement(4218..4415)
                     /locus_tag="VGE92_08445"
     CDS             complement(4218..4415)
                     /locus_tag="VGE92_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313893.1"
                     /translation="MTRLFALTLVLVSFAGTASAAHEKHFKDPRPAVSAPEIDPASAL
                     SGVTMLLGGLAVMRGRRGSKQ"
     gene            complement(4613..5254)
                     /locus_tag="VGE92_08450"
     CDS             complement(4613..5254)
                     /locus_tag="VGE92_08450"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY1313894.1"
                     /translation="MIRRTALAAWFALASVPLHATTYTLEPDYTQGVFRWNHLGFSSP
                     AAQFAQGEGTLEFDRADPTRSSVTVTIPLESLRTGVAGLDEHFRSADFFDTAKFPSAT
                     FRSTHVEKGPAADQLKVTGDLSLHGVTQPVTLDVTVVKIGSNPRTGLATIGFDAATTL
                     KRSAFRLGKYVPQVGDEIQLRITSQAVDAQAYAQYLKTQAAAEAAAGSSPTKK"
     gene            5393..6316
                     /locus_tag="VGE92_08455"
     CDS             5393..6316
                     /locus_tag="VGE92_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY1313895.1"
                     /translation="MNTDVMPLRIGEWRVDPASGQISRNGANVRVDVRTMRLLLCLAE
                     RAGQIVSIDDLLTRVWSGVIVTQDSVYQAVASLRRVLGDDIKQPQYIETVPRLGYRMV
                     AGVSPWADEPAAPRSPAAAPITVQPTPALRRNATALWAVVAALCIALLAAFLSHRDFA
                     GHPAAASSPPQKSVAVLPFLDLTTQAMSEEYFADGMTEELIDKLSKIPGLRVPSPTSS
                     FFFKGKQLTVAQIAQSLGVAYVIDGSVRKSDATLRVAARLVRANDGYVVWSETYDRAF
                     DDKLQVQADIAGEVAKASAKALAKALRESIN"
     gene            complement(6309..7412)
                     /locus_tag="VGE92_08460"
     CDS             complement(6309..7412)
                     /locus_tag="VGE92_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313896.1"
                     /translation="MAGCYLLLASIAGCSLISLKSPEKPLSKRDLDARILTREYSARF
                     IAAVAQTADEIAAGAPDSATALNALKWKIAATGQSQRAASQMAPMMGVLDTWALSLQM
                     SNYLATGEGRDLFDKEQPLAVALAADLAREAENLTRRLTTPDEFVKDQRFVLEYAASN
                     PIESLHFARASVVDLWTRDSGAGTKLVDSLGTVPEALADAGDRLRMYGQTGPSLVLWQ
                     AELAAQEAGLGGKDVGAALQRLDERVARLYELADATPKMVNGMVRDAGVRFDASWAEM
                     MRDVRDEGMSLSGNLSVERQAALNALEMQRTAVTADVDRISKQMISQAGDEVRRLVRE
                     ALLLLIALVLVLLGVPFVAGYSVGRARASRPPS"
     gene            complement(7544..8227)
                     /locus_tag="VGE92_08465"
     CDS             complement(7544..8227)
                     /locus_tag="VGE92_08465"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEY1313897.1"
                     /translation="MRAATSTGAQAAALAAIQSIRALPVTNTPALRKIRRQWSAALKS
                     QAPAYVLRFAEHLLSHASWPARLVAYEVIAAHPATFATLTHRHIERMARGLADWGSID
                     LFGVTVSGPAWRADRLSTLQVRTWARSRDRWRRRLALVSTVPLNVPSRGGRGDAARTL
                     DICARLLDDREPMVIKALSWALRALAKRDPNAAEKFLSRREHPLPARVLREVRNVLDS
                     GVKRPSRSH"
     gene            complement(8224..9501)
                     /locus_tag="VGE92_08470"
     CDS             complement(8224..9501)
                     /locus_tag="VGE92_08470"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1313898.1"
                     /translation="MSRTFRSLHSYNYRLWAGGALVSNVGTWMQRTAQDWIVLTQLTH
                     NNATALGIVMALQFGPQAVLLPVTGYAADRLDRRKLVLATQTAMGVLALGLGVLTVGG
                     WVKLWEVYVFALALGCVSAFDAPVRQTFVAELVIEEDLANAVALSSASFNVARLIGPA
                     IAGVLIAGVGSGWVFLINAASFAAVIGSLKALHVDEIHLRNKALRTRGSLAQGFKYIW
                     KRPDLVAVLLMFFLIGTFGINYPIFIATMSVTVFHKGASEYGLLTSMMAVGSVVGALL
                     AARQSRPDNAILVAATAIFGVGFTLAALMPNYWLFGLVLTVVGVAAQNFTTSANSMVQ
                     LSTEPAMRGRVMAILLALAMGGTPLGAPIVGWVADTFGPRWSLAVGAASGFAAALVGI
                     YYLAKYRNLRVRFAAGRIRVNLDAQRLMNGGAR"
     gene            complement(9553..11637)
                     /locus_tag="VGE92_08475"
     CDS             complement(9553..11637)
                     /locus_tag="VGE92_08475"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY1313899.1"
                     /translation="MKIKMGILSGAAALFTVLPAGANSGAPRRLDLADVAAVVRVSEP
                     QLSPDGKSIVFVVARPNVKEARYDKSLVLVDVAGGSERVLTYDRKGVSSPRWSPSGQA
                     IAFLDSAQPTPAAAPAAGAAPAPPEKPPGAGSPKAEIFIMPMNGGDAHRITNSAQDVE
                     QFAWSPDGREIAYAVADENPNQAEIDKHNDAFQVGDNDYLATAAALPSHLWLMPSSGG
                     AARRLTSGTWSLPKGAPPSSPASPLAWSADGKSIAIVQQSTPNWGDSDLAVVAIVDVA
                     SGQIRKLTAHQALEGYPVYSPDGSRIAYWYSRDGDPNNETDLFVTASSGGNGVDLTRT
                     LDRSIVRAEWSGDGKSLLVGAHDGVRASLWLVPQSGPAQKINLGNVDPAWSYWVDLSF
                     GKDGALAFAGNTPTHPNEIYYLAPGGAQPRCLTSFNQEIAARDLGRAEQFDWKGPNGY
                     REDGVVVYPPNFDPKRKYPLVLVIHGGPQSASTLVFSPLAQLLAAHDYIVFSPNYRGS
                     DNLGNAYERAIFNDAGAGPGRDVIAGIEALEQKGFIDKSRIAVSGWSYGGYMTSWLIG
                     HYGIWKAAVSGAAVNDLLHEYALSDNNVTVRYNFGGPPWSDKLLKAYQEQSPISYASH
                     ISTPTLILSDTGDARVPISESYLMYHALKDRNVPVEFYAYPVGGHFPSDPVRGPDVYR
                     RWVDWIDRHLSP"
BASE COUNT         2235 a         3663 c         3719 g         2196 t
ORIGIN      
        1 tatccgccca gctgccaatc agattgcgac cgaattattt caggcgcgcc gtggtgaaca
       61 aacgcggtac ggccttcaag ttgtcgcgta tcgcgccaaa agcgacgtga ccggtgccgc
      121 ttacccccgt ttctgtggca taggcaatgt ccttcgacta acaatcgcta aataagttgc
      181 ttaagtatgg ccgcgcgtca gccagcgacg ggcagactgg gagcaatact tcgaccagct
      241 cggcgagtat ctgaacactg tcgaaaaggg cggaggaact catggcaaag acaagtagca
      301 gtccgcgctc ggtggtgcac gcgacgttcc gcagcgaacg gacctacccg gtttctgcgg
      361 ctcaggtgtt caaagcgctg gccgatccgg cagccaaggc gaagtggttc acgggtggcg
      421 acggctacac gctgctggcg cgcgagatgg atgtgcggcc cggaggccgc gagcacgtga
      481 aggggcgcct ccagactggc gtcgtgacga cgttcgatgc cacttaccat gatgtcgtgg
      541 tggaccaacg gatcgtctat tcctacgaga tgcacttgga cgatcgtaaa atctcggttt
      601 ctctggccac gttggaactt aaaccgcagg gcagcgctac ccgccttgtg atgaccgagc
      661 agggagcgtt tctcgatggc tatgacgatg ccggatctcg cgagcatggc tccaattatc
      721 tgctcgacgc gctgggagaa tcgctccaca tgccgggcac tgtatgaatt cgcggttttg
      781 atcccaaatg ggttttaacg gaaattagta tgtccttgac gctctacgct cacccgttcg
      841 ccgcctattg ctggaaagtg ttgatcgcct tgtatgagaa tgcgacgccg tttcgctatc
      901 gattggtcga ggacgccgcg ggctgggcgg aattggcgac gctttggccg atcaagaaat
      961 ttccggtgct gcgcgacggc gacgcaacga ttgtcgaatc cagcatcatt gttgaatact
     1021 tgatgcatcg ctatcccgga acggcgcgaa tgattccggt ggatgacgaa gcagcgctcg
     1081 cggtgcgttt catggatcgg tttttcgata actacatcat gacgccgatg caaacactgg
     1141 tcgcggatcg aatgcgaacg gacaccgagc gcgataccaa aggcgtcgcg gatgcccgga
     1201 aaatgctgga tgtcgcgtat ggctggctcg aggagaagct ggtgcccgac tcttgggcat
     1261 gcggtgactc cttctcgttg gcggattgtt ccgcggcgcc atcgcttttc tacgccgatt
     1321 gggtacatcc gctcggcgat cgattcccgg gggttctcgc ctaccgcggc cgggttctgg
     1381 ccaggtactc cgtcgcccgt gtcattgatg aggcgcgccc attccgcaag tttttcccgc
     1441 agggagcgcc cgatcgggac tgacaaaggc cggcaacggc ataaattcgg atcgcgcgcc
     1501 catcacagca accgcggacc gcgccgccgc aaaagccgct gcgcgttgct tggggtaggc
     1561 tatgggggca ctgacaacgg ggctttgatt ggctgaccgt gccgaaacag aaataccgcg
     1621 gcgacccgcc gatagttcga aatccgcaga gcccgcggtg gcggagtcac attccacgcc
     1681 ggagatctca gcgcctatgc tcgacgtcca cgctccccac gaggccgtcc atacgtggaa
     1741 ggacttcttc atccatatcg ccacgatcgt catcgggctg ctcattgctg tcggcctcga
     1801 gcagaccgtc gagtactttc accatcgcca acaagtcgcg gagatccgtc gctctctcgc
     1861 cgaggagcgc cgcataaacg aggttatctt taccggtgcc tgcaatgaat tccgccgcta
     1921 cgctcccatt ttgctcggca gcttgcagac cctcgcctat ctgcggaccc acccaggcgc
     1981 tccacccagc cagtggccag gccggttcag cttctacatc ctcaccatcc atttccaaga
     2041 ttcagcgtgg aagaccgctc tgcaaagccc cgcgctcgcg tacatgccgc gcgttgaggt
     2101 cggaatctac agcgatatct acgaccgtct ggccaaggtg agcgaacaat ccttggccga
     2161 gttccacgcc gtcattcgcg caaagagctt catgctgcag ctaaccgatc ctgccatgtt
     2221 cgattcggag caaagcatgc aggcctacgc ccgcgtctcg gatgtcgtca caagcctata
     2281 tctcatgggc attggtgagc gcaacatggc gcgcctttac agtgactttc gcggtcctcc
     2341 aacggacctg gaactctatg ccctcattcc gccagcgccg gcgaagcagg acgtcgatgc
     2401 tgttcgcgag atcgccgcgc ccatggagcg cgagaaataa agatcccggt cgtgagatag
     2461 actttgacga acttcggcga ggggagccaa tgggaacagc agcgaaatca ccactgacgc
     2521 ggcgctcctt gatattcgga ctcgcggcgc tgcccgcaat ggccgcccgc gcctgggcag
     2581 acgatgtcga gctgccctcg cgtctctttt tcaaggaacc tcccgcggtc aaagcggcag
     2641 accaggcgct caatgtcatg gaattcgagc cgttggcgcg cgccgcactg ccgccggctc
     2701 actttgggta tatcgcaacc gggtcggatg acgatcgcac gctcatccaa aaccatgagg
     2761 cctacaatca ctaccaaatc cgagcccacc gcttcagtga tttgagccga ctgcgcacca
     2821 ctcgttccgt gttcggcgcg caatggccga gtccgattta tctgtccgcg gtgtcggcga
     2881 tgcgtgcttt tcatccggac ggtgaactgg cagtggcacg cgcagcacga agccgctcca
     2941 tgcagttgat gctctccacg ggatcatcga cgccggtgga ggcggttgcc gcagccgccg
     3001 ccgcgccgct ttggcagcag ctctatccga ccgacgattg ggccgtgacc accggaattg
     3061 tccggcacgc tgaacgtgcc gggtgcaccg caatcgtact cacggtcgat tcccacgggg
     3121 tccggaacaa tgaaacattg aagcgcgcca tgctgtcgga cagccgcacg tgtagcgctt
     3181 gccatccggg aaactcgcac gacatgtggc gtcgaggacc gatgtttgcc gaccttgacg
     3241 tatcgcgcgt gaccgctctc gcacccgcgg cgttgaccgt cgcgtttctc gaccggctgc
     3301 gcgccgtggt caaaggccgc ttgatcgtca aaggcgtcgt caccggcgag gatgcggcgc
     3361 tggcggtcga acacggcgcc gacgccatcg tgatttccaa tcatggcggg cgcaatgaag
     3421 agactttgcg agccacggtg gattgcctgc cggaagtcgt cgctgccgtg cgcgcccgag
     3481 ttccggtgtt tctcgacggt ggaatccggc gcggcacgga cattttcaaa gcgctggcac
     3541 tgggagcgac cgcggttggc atcggcagac cccaggcttg gggtcttgcg gcgtttggac
     3601 agccgggggt cgaggcggtg atcgatatcc tgaatcgcga acttgctgcg atcatgcgcc
     3661 aggccgggac cccgtcgatc gaggacattg tgggcgcgga cgtgattccg acgatgcgtg
     3721 cttagcggag cgcgggtcga gccatccggc cgctgggcaa tgagaatcct tcgagacagc
     3781 agcaactttt cccagcctgc ggtgttctgt gggacaagcg ggagtcaatg atcgctggct
     3841 cctgggtggc aggacacatt tatcagaagg ggattcttgt atgacatcgc caatgacggt
     3901 gaagcgggtg ctggtagctc aattgtttgg ccttgtgctg ctggtcgcgg cgaaccaggc
     3961 gggggcgaag ggttgcgtca agggtgccgt ggtcggtgcg gttgcagggc acgtggccgg
     4021 gcaccacgcc gttgtcggtg ccgcggtcgg ttgcgcggcg gggcatcatc tcgcgaagaa
     4081 gaaacaggaa gagcaacgcc agcagcagcc cgcgccgggg caacagacgt aaggattagc
     4141 gaggaacggt gcccgcaaaa tgaatcgggc cgcacgaggc ggcccgattc atgaacgtcc
     4201 tgaactctct gctgcgctta ttgtttgctg ccgcgacggc cgcgcatgac ggccaatccg
     4261 ccgagcagca tcgtcacgcc gctcaaagcc gaagcggggt cgatttccgg cgccgaaacg
     4321 gcaggtctcg ggtccttgaa gtgcttctcg tgcgccgcgc tggcggtgcc cgcgaaactt
     4381 accaacacca atgtcaaagc gaagagtctg gtcacgaaac ggtctccctt gtaggcaaaa
     4441 attcgcgccg tcaattgttg aaccgtcgcg attgagagac ataatagacg acacgcgtca
     4501 caaaacgatg tctcattccg caaattagat cgagttatgt aaaatcgatt cgagatattg
     4561 tgtgatgcgg atcacaaatt tcaaatgtcg tgatttgcgg cattcggcgg gctcattttt
     4621 tcgtcgggct gctgccggcc gcggcctccg cggctgcctg cgtcttcaaa tattgcgcgt
     4681 acgcttgcgc atcgacggct tggctggtga tgcgcagttg gatctcgtca ccgacttgcg
     4741 gcacgtactt gccgagacgg aaggccgaac gcttgagcgt ggtcgcggca tcgaagccga
     4801 tcgtcgcaag accggttcga gggttcgagc cgatcttgac gactgtgacg tccagagtaa
     4861 ccggctgggt tacaccatgc aggctaagat cgccggtgac tttgagctga tcggccgccg
     4921 ggcctttctc aacgtgcgtg cttctgaagg tcgcgctcgg gaatttggcc gtgtcgaaga
     4981 aatccgccga cctaaaatgt tcgtcgagac ccgctacacc cgtgcgcagg ctctcgagcg
     5041 gtatcgtcac cgtgacggac gaccgcgtcg gatcggcgcg atcgaattcg agcgtgccct
     5101 caccctgagc aaattgtgcg gcggggctcg agaatccgag gtggttccat cgaaacacgc
     5161 cctgggtgta gtccggctcg agcgtatagg tggtggcatg gaggggcacg gatgccagag
     5221 caaaccaggc agcgagggcg gtgcggcgaa tcattgggcg aactcctgtg gttggggtaa
     5281 attggcctcc aatcttgcga cgcgcgatta cggaagtcct gaatcgctcc tgaaaagccc
     5341 tgaatgactc tgaaatgcgc cgccgtcacg ccgcccgaga gccgatcgaa aaatgaatac
     5401 agacgtcatg cccttgcgca ttggcgagtg gcgggtggat cccgcttccg gccaaatctc
     5461 ccgcaacggt gcgaacgtcc gcgtcgatgt gcgtaccatg cggctgctgc tgtgtctcgc
     5521 cgagcgcgcc ggccaaattg tcagcatcga cgatttgctc actcgagtct ggtctggggt
     5581 catcgtcacc caagattccg tttaccaagc agtcgcctcg ctgaggcgcg tgctgggcga
     5641 tgacatcaaa cagccccaat acatcgagac cgtaccccgg ctcggctatc gaatggtcgc
     5701 tggggtcagc ccttgggctg atgaaccggc tgcgccacgc tcgccggccg cggcaccgat
     5761 cacggttcag ccgacgcccg ccttgcgccg caatgccacc gctctctggg ccgtcgtcgc
     5821 cgcactctgc atcgcgctcc tcgccgcttt tctgtcgcac cgcgactttg ctggtcatcc
     5881 ggcggccgcc tccagcccgc cgcaaaaatc ggttgcagta ctgccgtttc tcgatctgac
     5941 cactcaggcg atgagcgagg agtattttgc ggacggcatg accgaggaac tgatcgacaa
     6001 gctgagcaag atccccggac ttcgcgttcc ctcgccgacc tcttcattct ttttcaaggg
     6061 caagcaactc acggtggcgc agatcgccca gtcgttgggc gtggcctacg tcatcgatgg
     6121 cagcgtgcgc aaatcggacg cgacgctgcg ggtcgccgcg cggctggttc gtgccaacga
     6181 tggttacgtc gtgtggtctg agacctacga tcgagcgttc gacgacaagt tgcaggtaca
     6241 ggccgacatt gccggcgaag tggcgaaagc ctcggcgaaa gccttggcaa aagccttgcg
     6301 ggaatcgatc aactaggcgg acgcgaggcg cgggcgcgtc cgacggaata gccggcgacg
     6361 aacggtacgc cgagcagcac cagcaccaaa gcgatcaata gcagcaacgc ttcgcggacc
     6421 agacggcgca cctcgtcccc cgcctggctg atcatctgct tcgagattcg atcgacatcc
     6481 gcggtgacgg ccgtgcgctg catctccaag gcattgagcg cggcctgccg ttcgacgctc
     6541 aagtttcccg acagcgacat gccttcgtcg cgaacgtcgc gcatcatctc cgcccaggag
     6601 gcatcgaagc gcacacccgc gtcacgcacc atgccattca ccattttcgg cgtcgcatcg
     6661 gcgagttcgt acaggcgggc gacccgctcg tccaggcgtt gcagcgccgc cccgacatcc
     6721 ttgccgccga gtcccgcttc ctgcgccgcc aactcggcct gccacagcac cagagagggc
     6781 ccggtctggc catacatgcg caggcgatcg ccggcgtccg ccagcgcttc ggggaccgtg
     6841 cccaaagaat cgaccaattt cgtacccgcg ccgctatcgc gggtccacag atcgacgacc
     6901 gaggcgcgcg caaaatgcag gctttcgatg ggattcgatg cggcatattc caggacgaag
     6961 cgctggtcct tcacgaattc atccggcgtc gtcagtcggc gggtcaaatt ctcggcctcg
     7021 cgcgcaagat cggcggcgag cgcgacggcg agcggctgct ccttgtcgaa caggtcgcgg
     7081 ccttcgccgg tcgcaagata attcgacatt tgcagcgaca gtgcccaagt atccagcaca
     7141 cccatcatgg gggccatctg gctcgccgcg cgctggctct ggcccgtggc ggcgattttc
     7201 catttcaatg cgttgagcgc cgtcgcggaa tccggcgcgc cggccgcgat ttcgtccgcg
     7261 gtctgcgcga ccgccgcgat gaagcgcgca gagtattcac gcgtcagaat ccgcgcgtcg
     7321 agatcgcgct tcgacagagg cttttccggg ctcttcaatg aaatcagcga gcagcccgcg
     7381 atgcttgcga gcagcaggta gcagccggcc aagcgaaccc atcggcgagt gattgtggca
     7441 acggtgccca acggcgattc ctcggcagga tccagtaatg cccgggacgt taggccatcc
     7501 gattcggttg cgcaagccgt gcggccgctg cgcaagtgcg ttatcagtgc gatcgcgacg
     7561 gacgcttgac tccggagtcg agcacgttgc gaacctcgcg cagcacgcgc gcgggcaggg
     7621 ggtgttctcg acggctaaga aatttctcgg ctgcgtttgg atcgcgcttg gccagggcgc
     7681 gcagcgccca cgacagcgcc ttgatcacca tcggctcacg atcgtcgagc aagcgagcgc
     7741 agatatcgag cgtgcgtgcg gcgtcgccgc gaccgccgcg gctcggcacg ttgagcggca
     7801 ccgttgacac cagggcaaga cgccggcgcc atcggtcgcg agaacgagcc caggtgcgca
     7861 cctggagcgt ggacaggcgg tccgcgcgcc acgcaggtcc gctgacggtg acgccgaaca
     7921 gatcgatcga cccccagtcc gcgaggccgc gcgccatccg ctcgatgtgc cggtgagtga
     7981 gcgttgcaaa ggtcgccgga tgagcggcga taacctcata ggcaaccaag cgcgcgggcc
     8041 aggacgcgtg actcagcaaa tgctcggcga aacgcagtac gtaggcgggc gcttgcgatt
     8101 tcaaagcggc cgaccactgg cgccgaatct tgcgcaacgc cggagtattc gtcaccggca
     8161 acgcgcgaat cgattgtatt gccgccagcg cggcagcttg cgccccggtt gacgtagccg
     8221 ctctcatcgg gctccgccat tcatcaggcg ctgtgcatcg aggttgacgc gaatgcgacc
     8281 cgctgcgaag cgcacgcgca agttgcggta tttcgcaaga taatatatgc cgaccagcgc
     8341 cgcggcgaat cctgaggctg cgccgacggc caacgaccaa cgcggaccga aggtgtcggc
     8401 aacccagccg acgatgggcg cgccgagcgg cgtgccgccc atcgccaatg cgagcagaat
     8461 cgccatcacg cgcccgcgca tcgcaggctc ggtcgacaat tgcaccatgc tgttcgccga
     8521 tgtggtgaag ttctgtgccg caacgccgac gacggtgagc accaggccga aaagccagta
     8581 attcggcatc agggcggcga gcgtgaagcc aacgccgaag atcgcggtag ccgcgaccag
     8641 aatcgcgttg tcaggccgcg attgccgggc ggcgagcagt gcgcccacga ccgaaccgac
     8701 tgccatcatc gaggtcagta gcccgtattc gctggcgccc ttgtgaaata cggtgaccga
     8761 catggttgcg atgaagatcg gatagttgat gccgaatgtg ccgatgagaa agaacatgag
     8821 gagcacggcg acgaggtcgg gccgtttcca gatgtacttg aaaccttgcg cgaggctgcc
     8881 tcgggtgcgc agcgccttgt tgcgcaggtg aatttcgtcg acgtgcaacg ctttgagcga
     8941 gccgatgacc gcggcaaacg aggccgcgtt gatcaggaac acccatccgg atcccacgcc
     9001 ggcgatgagc acgccggcga tggccggacc gatcagccgc gcgacgttga acgacgccga
     9061 actcaatgcc accgcgttcg ccaagtcctc ctcgatcacc aattccgcca cgaatgtctg
     9121 gcgcaccggc gcgtcgaacg cagacacgca gccgagtgcc aaagcaaaca cgtacacctc
     9181 ccagagcttg acccacccgc cgactgtcag gacgccgagc cccagagcca gtacccccat
     9241 cgccgtttgt gtggccaaca ccagcttgcg gcggtcgaga cggtcggcgg cataaccggt
     9301 caccggcagc agcaccgctt gaggtccgaa ctgcagcgcc atcacgatgc cgagggcggt
     9361 tgcattgttg tgggtcagtt gtgtgaggac gatccagtcc tgcgcggtgc gctgcatcca
     9421 ggtcccgacg ttggacacga gggcgccgcc ggcccatagg cgatagttgt agctgtgcag
     9481 cgagcgaaag gttctgctca ctcgagcgcc ccagtcatgc gcgggcgtta tcgagtcggc
     9541 tgaaggacat gctcagggac tgagatggcg atcgatccaa tcgacccagc ggcgatagac
     9601 atccggcccg cgcaccggat cacttgggaa gtggccgcca accggatatg catagaattc
     9661 gaccggcacg ttgcggtcct tgagtgcgtg gtacatgaga taggattcgc tgatcggcac
     9721 gcgggcatcg ccggtgtccg acaggatgag cgtcggtgtg ctgatgtgag aggcgtaact
     9781 gataggagac tgttcctgat aggccttgag cagcttgtcg ctccacggcg ggccgccaaa
     9841 attgtagcgc accgtcacgt tgttgtcgga cagggcatat tcatgcaaca ggtcattcac
     9901 ggccgcaccc gacaccgctg ccttccagat gccatagtgg ccgatcagcc acgatgtcat
     9961 gtagccgccg taggaccagc cggacacggc gattcgcgac ttgtcgatga agcctttttg
    10021 ctcgagagcc tcgatgcccg cgatcacatc gcggccgggc cccgctccgg cgtcattgaa
    10081 gattgcgcgc tcgtaggcgt tgccgaggtt gtcgctgccg cgataattcg gagagaagac
    10141 gatgtagtcg tgcgcggcga gcagctgcgc cagcgggctg aacacgagtg tcgacgcgga
    10201 ttgcgggccg ccgtgaatga cgagcaccag cgggtacttg cgttttggat cgaagttcgg
    10261 cggataaacg acgacgccat cttctcggta gccgttggga cctttccagt cgaattgctc
    10321 cgcgcggccg agatcacgcg cggcaatctc ttgattgaag gaggttaggc agcgcggctg
    10381 cgcgccgccg ggagcgagat agtagatctc gttggggtgt gtcggcgtgt ttcccgcgaa
    10441 tgccagggcc ccgtctttgc cgaacgacag atcgacccag tagctccatg caggatcgac
    10501 attgcccagg ttaatttttt gggccggccc cgactgcggc accagccata gagaagcgcg
    10561 caccccgtca tgcgcgccga ccaagagtga cttgccgtcg ccgctccact ccgctcgaac
    10621 gatgctgcgg tcgagcgtgc gggtcagatc aacgccgttg ccgccgctgc ttgccgtgac
    10681 gaacagatcg gtttcgttgt tggggtcgcc gtctcgcgaa taccagtaag caatgcgcga
    10741 gccgtccggt gaataaaccg ggtagccctc gagcgcctga tgggcggtaa gtttgcgaat
    10801 ctgaccgctt gcgacatcga caatcgcgac gaccgccagg tccgagtcgc cccagttcgg
    10861 tgtcgactgc tgcacgatgg cgatcgactt gccgtcggcc gaccacgcga ggggcgaggc
    10921 cggtgaactc ggcggcgcgc ccttcggcag gctccaggtt cccgaggtaa ggcgccgcgc
    10981 cgcaccgccg ctcgacggca tgagccacag gtgcgagggc aatgctgccg cggtcgccag
    11041 gtagtcattg tcccccacct gaaacgcatc gttgtgtttg tcgatctccg cttggttcgg
    11101 gttctcatct gcgaccgcgt acgcgatctc gcggccgtcc ggactccagg caaactgctc
    11161 cacatcctgc gctgaattgg tgatccgatg cgcgtcgccg ccattcatgg gcatgatgaa
    11221 aatttcggct ttcggcgagc cggcgcccgg cggcttctcg ggaggcgccg gggcggcacc
    11281 ggctgcaggc gccgcggccg gcgtgggttg ggcgctgtcg agaaacgcga tggcctggcc
    11341 cgaaggcgac caccgcggac tgctgacgcc tttgcggtca tacgtcagca ctcgctcgct
    11401 gcccccggcg acgtcgacca ggaccagcga cttgtcgtag cgcgcttcct tcacgttcgg
    11461 ccgcgcgacc acgaatacga tcgacttgcc gtccggagag agctgcggct cggacacgcg
    11521 cacgacggcg gcgacatcgg cgagatcgag gcgccgcggc gccccgctat tcgctcccgc
    11581 gggcaggacg gtgaacagtg cggccgcgcc cgacagaatt cccatcttga ttttcaaggt
    11641 ctcgacttcc ctgcaggctt ggcctagaag caacgaagcg tcacggatga cgcgacccag
    11701 cggcggcggt tgcgcagcat ggccagagcc gccagctgca aagtaatact agttcatgcc
    11761 gcgcttgttc gcatttctcg atggacgcac ccgatatcgc cgttgatcgt cat
//
LOCUS       DASWZT010000311         1875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_65281, whole genome shotgun sequence.
ACCESSION   DASWZT010000311 DASWZT010000000
VERSION     DASWZT010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65281"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="VGE92_08480"
     CDS             complement(<1..1171)
                     /locus_tag="VGE92_08480"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HEY1313900.1"
                     /translation="MGDNVRYWIATAPVGAASVLAEELALFGALDIRERSHDVKFQGT
                     LEIGYRACLWSRTATRVHLSLGSIDAGSASNLYEAVKRIDWREHLAPGATLACDCSGG
                     NESIRHTIYGSQLLKDAVCDNLRASTGERPNIKPERPDVLLHLHVEGATALLSVDFSG
                     ESLHRRGYRAEGGRAPLKENVAAAVLLRAGWPGIAAQGGILVDPMCGSGTFLTEGALI
                     AADAAPAFDREYFGFAGWRGHDRELWEQLRAEARARRAARAPRRCIFGSDADAEAVRT
                     SLAHGVNAGVAEWLHVEKRALSELTRPHGDPGLIVTNPPYGERIAAESGLAALYAELG
                     ATLRERFQGWQAAILTGNPPLARNLKIYAKRTHRFFNGNIECRLLRFDLDEAHEQR"
     gene            complement(1217..>1875)
                     /locus_tag="VGE92_08485"
     CDS             complement(1217..>1875)
                     /locus_tag="VGE92_08485"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEY1313901.1"
                     /translation="VQCCRRTIIQPNWGSRMHPLNSVKGAIVTGVVLAIIIGICLSVF
                     AGPGAAFHPIRIDRWVHIVSGVTWIGLLYYFNVVQTPGLAAAAADKGGPGGAGIAKYI
                     APRALLWFRWAAVATWLTGAIYLMVGYGNWSGIVWAFAMQPGFRTIGLGAWLGTVMLF
                     NVWVFIWPNQKKILGLVPASDDEKAAARKIAGRASRINFILSLPMLMCMGGQSHGLPF
                     "
BASE COUNT          346 a          637 c          590 g          302 t
ORIGIN      
        1 gccgctgctc gtgggcctcg tccaaatcga accgcagcaa ccggcattcg atgttgccgt
       61 tgaaaaaccg atgcgtgcgc ttggcataga tcttgagatt gcgcgcgagc ggcggattgc
      121 ccgtaagaat cgcggcctgc caaccctgaa atctttcgcg caaggtggcg ccaagttcgg
      181 catatagagc ggcaagtccg gattccgcag cgatgcgctc gccatagggc ggattggtca
      241 cgatgaggcc cgggtcgccg tggggccgcg taagctcgct cagcgcgcgt ttctctacgt
      301 gcagccactc ggcgacgccg gcgttcacgc cgtgcgccag cgacgtccgc accgcctccg
      361 catccgcgtc ggacccgaat atgcagcgcc gcggcgcgcg cgcagctcgg cgcgcgcgcg
      421 cctcggcacg caattgctcc cacagctcac gatcgtgccc gcgccagccg gcgaagccga
      481 aatattctcg gtcgaatgcc ggcgcagcat ccgctgcgat cagcgcgcct tcggtgagaa
      541 aggtgcccga accgcacatg ggatcgacga gtatgccgcc ctgcgccgcg atgccgggcc
      601 agcccgcccg cagcagcacc gcggcggcga cgttctcctt gagcggcgcg cggccgccct
      661 ccgcacggta tccgcgccga tgcaaactct cgccggagaa atccaccgac aacagcgcgg
      721 tcgcgccttc gacgtgcaga tgcagcagga catcggggcg ttcaggcttg atgttgggac
      781 gctcgccggt cgacgcgcgc aagttgtcgc aaaccgcgtc tttgagcagt tgcgagccgt
      841 agatcgtgtg ccggatcgac tcattgccgc cgctgcagtc gcaggcgagc gtcgcgccgg
      901 gagccaaatg ctcccgccaa tcgatgcgct tgacggcctc gtacaggttg ctcgcgctgc
      961 cggcgtcgat cgatcccaag cttaagtgca cgcgggtcgc ggtccgcgac cacaggcagg
     1021 cacgatagcc gatctcgagc gtgccttgga acttcacatc gtggctgcgc tcgcgaatgt
     1081 cgagagcacc gaatagcgcg agctcctcgg cgagcaccga ggcggcgcct accggcgcag
     1141 tcgcgatcca gtagcgcacg ttgtcgccca cggagcgggc gctctacagc gcggttcgtc
     1201 gcagcgcggc cgtcatctag aaaggcaagc catgcgactg gccacccatg cacatgagca
     1261 tgggcagcga caggatgaag ttgatgcgcg aggcgcggcc cgcaatcttg cgcgctgcgg
     1321 ccttctcatc gtcgctcgca ggcaccagcc cgagaatctt cttttggttc ggccagatga
     1381 agacccacac attgaacagc atcactgtgc cgagccaagc gcccaggccg atggtgcgaa
     1441 atcccggctg catcgcgaaa gcccacacga ttccgctcca gttgccgtac cccaccatca
     1501 ggtagatcgc acccgtcagc cacgtggcca ccgctgccca gcgaaaccag agcagtgccc
     1561 gcggcgcgat gtacttggct atcccggcac cgcccggtcc gcctttatcg gcggcggccg
     1621 cggccagtcc gggcgtctgc acgacattga agtaatacaa cagaccgatc caggtgaccc
     1681 cggacacgat gtgcacccaa cggtcgatgc gtatcggatg gaacgcggcc cccggacccg
     1741 caaatacgct caggcaaatg ccgatgatga tggcgagcac gacgcccgtg acgatggcgc
     1801 ccttgacgga attgagcgga tgcatgcgag atccccaatt tggctgaatt atggttcgtc
     1861 ggcagcattg tacgc
//
LOCUS       DASWZT010000312        24317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_60478, whole genome shotgun sequence.
ACCESSION   DASWZT010000312 DASWZT010000000
VERSION     DASWZT010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 24317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24317
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60478"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VGE92_08490"
     CDS             <1..367
                     /locus_tag="VGE92_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HEY1313902.1"
                     /translation="FKNAVLSLKVTPLITPDNRVILDLDVADDTVGAQVTSATGGSVP
                     SIDTREIITQVLVNDGQTVVLGGILDTTKSKSANKVPFFADIPILGNLFKSTININNK
                     TELLIFITPKILREGSNLY"
     gene            430..2331
                     /locus_tag="VGE92_08495"
     CDS             430..2331
                     /locus_tag="VGE92_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY1313903.1"
                     /translation="MCLTWVLEGCGGGSGAISGKGSTTGTTGTGTSGQTTTVLSLGSG
                     TGSSFQPGVIAISTKSLSAGGSTSLQVSLVDQTGAAYTTGTQITFSSPCAGQGLATIA
                     ATGVTPASPSVTTSTGTASATYVASGCSGNDVITAAATANDQSLTANGTVTVAQAAIG
                     SISFISATPSSITLKGVGSVGGSATSTVIFKVLDTSGGPRAGATVNFSLNTTVGGITI
                     APASATTDANGQVQTIVSGGTVATTVRVTATTTASGGGTISTESNALTVSTGIPTSAN
                     ISLAVKCQNVEAWNVDGVSVPVTVSMTDRFSNPVPDGTTANFQTTLGGIQASCQTGSA
                     TAGSGACSVNWVSKAPRTVNGNPQSTTGNSNTSAAYCSTPVGAALGVCGGTTNGRSAI
                     LVTAIGEESFKDANGSGFFNPADTVAWDATDGDNNFSNGQPKPWQDTSEPFLNEWELY
                     DSFGTPSFVLGEPFLDFNNNGKRDGPDGLVESALCEGPLCNTTGSSVAIGANNLIILS
                     GSHANSYVVSPAGGPPYHFNNGLSLAVDIFDDRMQQMPAGTTVAAAVGSGATASITSP
                     LPTAGWPCSSAAPYVDGQGNLIAGQLFNFTLAPANPAPAGGQVGGELYLTITTPAGLI
                     TTIAIPIQP"
     gene            2421..2948
                     /gene="aroK"
                     /locus_tag="VGE92_08500"
     CDS             2421..2948
                     /gene="aroK"
                     /locus_tag="VGE92_08500"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HEY1313904.1"
                     /translation="MLGKGNIFLIGPMGSGKTAVGRHLARMLRYTFHDSDADIEARTG
                     VDIAFIFEKEGETGFRIREKESIARLTLLESIVLATGGGAVIEEENRRVLAARGVVVY
                     LATSIDQQLERTRHARHRPLLNHTDPEHTLHELMQRRARLYAEIADLTVSTDGRRVQL
                     VAEEIHHELRRAPVS"
     gene            2969..4045
                     /gene="aroB"
                     /locus_tag="VGE92_08505"
     CDS             2969..4045
                     /gene="aroB"
                     /locus_tag="VGE92_08505"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEY1313905.1"
                     /translation="MHTLQVDVGHSRYPIAIGAGLLGDRELLAGSIRGHDLLIVTNTT
                     IGGLYLAKLAAGLDSRRIAECILPEGEQHKTLHTAGLVFDALVAKKMNRDATVLALGG
                     GVIGDIAGFAAACYQRGVGYVQIPTTLLAQVDSSVGGKTGVNHPGGKNLIGAFYQPES
                     VIADIDTLATLPDRELSAGLAEVIKYGCVWDPLLFDWLDNNMAALLARDVEALAYAIR
                     RSCEIKATVVAKDEREQNLRAILNFGHTFGHAIEAATGYETYLHGEAVGLGMLIASNL
                     SHRLGLIDAATEARIGAILVRAGLPTQAPRVGAAKALELMQMDKKVLAGALRLVLIEK
                     LGRAIVTGDYPQTALNATLAEYFA"
     gene            4042..5193
                     /locus_tag="VGE92_08510"
     CDS             4042..5193
                     /locus_tag="VGE92_08510"
                     /inference="COORDINATES: protein motif:HMM:NF002326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEY1313906.1"
                     /translation="MSDLAAYAALDEHSRGRRHPEPKPAYRSEYQRDRDRIVHSTAFR
                     RLVYKTQVFVNHEGDLYRTRLTHSLEVAQIARTIARALKLNEVLSEAICLAHDLGHTP
                     FGHAGQDALNECMLDFGGFEHNLQSLRVVDELEEHYAEFDGLNLSFETREGILKHCSH
                     SNALQLGELGQRFIHRQQPGLEAQLANFADEIAYNNHDVEDGIRAGLVSVEQLLAVPL
                     FREYHAAVHAKYPALAGRRQVYEILRRMINHLVTDLIDSSAARLAAAAVRSIEEVRAH
                     PQPLIGFSDQTRELNYALKSFLREHVYRHYKVRRMTAKAQRVIRELFDAFFGDPGLLP
                     EEHKATVSRLEMLQGAAGRARAVADYIAGMTDRYAILEHRRLFDPGERT"
     gene            5204..6262
                     /gene="hemE"
                     /locus_tag="VGE92_08515"
     CDS             5204..6262
                     /gene="hemE"
                     /locus_tag="VGE92_08515"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEY1313907.1"
                     /translation="MNEPLKNDLLIRALLREPTPRRPIWLMRQAGRYLPEYRATRARA
                     GGFLEMCTNPEIACEITLQPVNRYPLDAAILFSDILTIPHAMNLGLEFEAGEGPKFER
                     PVRSAADIAKLGVPDPGRELKYVIDAVALVRRELHGRIPLIGFAGSPWTVATYMVEGG
                     GSKSFGQIKRMMYEAPRDLHRLLALLAKATVLYLNAQIAAGAQAVMLFDTWGGVLTPA
                     QYEEFSLRYMAEVVDALTRKAETRRVPSIVFTKGGGAWLDKIAAIGCDAVGVDWTTDL
                     AAARRAVDGRVALQGNLDPAALFAAPETLRAETLKVLASYGAGAGHVFNLGHGITPDV
                     DPERVAVLIDTVQGYGVV"
     gene            complement(6247..7950)
                     /locus_tag="VGE92_08520"
     CDS             complement(6247..7950)
                     /locus_tag="VGE92_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY1313908.1"
                     /translation="MDRNWLTQYPPGVPTDIDPDSYASLRDIFEQACAEHRHSPAFSN
                     LGASLSFAQLDALSRAFAAWLQQKSGLVRGDRVALMMPNILQYPIALFGVLRAGMVVV
                     NTNPLYTARELEHQLVDSGAKAIVIVENFMHVLQQVLPHTELKKVLVTRIGDLLGVPR
                     GTIVNLVLKYVRKQIPEWKMPGAMTFKSALGGGLGLKLEALPLGPEDIAFLQYTGGTT
                     GVAKAATLTHRNMVANVLQASAWITPSLAAGEPQVVVTALPLYHIFALTTNCLAFIAL
                     GAHNILITNPRDFKAFVAELKKHKFNFISGVNTLFNALLHTPGFESVDFSALRVTFAG
                     GMALQAVVAQRWKEVTGCVITQGWGLTETSPIATANPIGLDFNGSIGLPIPSTDISIR
                     DDAGKEMPVDAVAEICVFGPQVMRGYWNRPDETEKVMFGDWLRTGDIGRMNAAGFVFI
                     EDRKKDMILVSGFNVYPNEIESVAAALPGVMEVAAVAQPDENSGEVVALFVVKKDPSL
                     TAEALIAYCRKELTGYKVPKHVYFRSELPKSNVGKILRRELRDELRRAAGATPQAQTT
                     P"
     gene            8017..8769
                     /locus_tag="VGE92_08525"
     CDS             8017..8769
                     /locus_tag="VGE92_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY1313909.1"
                     /translation="MNAAPDAREQQIIRSWNVNAVPWAEAIRSGSIESRKLVTDRAII
                     DAVLGLGPRRILDLGCGEGWLARALAACAIQTVGIDVVPELIAAAARLGGGEFLVREY
                     REIAQARWRAREFDAAVCNFSLLGKESVESLLAAIPEYLAAPGYLIVQTLHPLAACGD
                     LPYEDAWRAGSWLGFGAQFSEPAPWYFRTLESWHCMLRRTGFEVLECREPTAPGARAP
                     ASVIFICKPRVREQTARTAPDSVPVSSGNSTQ"
     gene            8821..9228
                     /locus_tag="VGE92_08530"
     CDS             8821..9228
                     /locus_tag="VGE92_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313910.1"
                     /translation="MNLWRLRRILRLASSGALLALSGCVIGYGPCLLQQPVKNNLTGR
                     VHFRDYPTSEGMDKVPVLALDRTAYVYAPAQSRHCLPANDVQLVGVAEFPQDVIENSH
                     VSVDGKLFQAGSSSQHTQFLMNVTTILPISGAR"
     gene            9285..9845
                     /locus_tag="VGE92_08535"
     CDS             9285..9845
                     /locus_tag="VGE92_08535"
                     /inference="COORDINATES: protein motif:HMM:NF024001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3761 domain-containing protein"
                     /protein_id="HEY1313911.1"
                     /translation="MKNANLAGLLAAPLMLLALSLPALADSPTPTPTTCKDGTTSTAT
                     GKGACSGHGGVQKAAATATAPAAAAPAMPAGGAPAASGATATCEDGTKSTATGKGACS
                     GHGGVLKPSKSAAPAAAPAPGTAMTAAPAAAAKTSTATKSAPASSTDPTGATAKCKDG
                     TYSKSTHKAGTCSSHGGVAEWLTAAQ"
     gene            complement(9910..10524)
                     /locus_tag="VGE92_08540"
     CDS             complement(9910..10524)
                     /locus_tag="VGE92_08540"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEY1313912.1"
                     /translation="MKRLLILWVLIAASVPARAETPVILVFGDSISAGYGLARVEQGW
                     VALLQTRLKEQEYGYQVINASVSGETTAGGLARLPRALDLHHPAIVVLELGGNDGLRA
                     LPVAPMRANLVRMVDLATAAGARVLLLGMRMPPNYGPDYTERFRLVYSEVARDKKLPL
                     VPFLLNDIALSPSLMQADGVHPNELGQPKLLANVWPSLLPLLRR"
     gene            10537..11211
                     /locus_tag="VGE92_08545"
     CDS             10537..11211
                     /locus_tag="VGE92_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003189864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1313913.1"
                     /translation="MQDQIVLEAKNVSKTVASPEGQLTILADVSLSVRTGETLAIVGA
                     SGAGKSTLLALLAGLDSPSTGRVLIAGVDLTQLDEDGRAAIRGKHVGFVFQSFHLIPS
                     LTAVENVMLPLELGQSRDARQVASAALAQVGLTPRLAHYPKQLSGGEQQRVAIARAFV
                     THPAVLFADEPTGNLDTATGQRITDLLFDLNRSTGSTLVLVTHDRELAARCGRVLELD
                     AGHTVN"
     gene            11212..13701
                     /locus_tag="VGE92_08550"
     CDS             11212..13701
                     /locus_tag="VGE92_08550"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY1313914.1"
                     /translation="MQVLRFALRNLWRDLKSGELSVLLLALTVAVLSLTAVGFFTSRI
                     SQGVRAQAGEVLAADLRLESPRPIAPRYFAEAQRRGLRTASILSFPTAIFSGDVSQLA
                     ALNAVTAGYPLRGRLRIADAPFGAARATDRIPGRGQVWIDARIIAQLKIELGATLKIG
                     AASFRVTQVLDYRPDQGTGFVNLAPAALLNYDAVASTQLIQPGSRVTYAALFAGPAQA
                     VSDFHEYLVSTKAPGERLREIDESSRQLNSAIDRSSRFLNLASLASVLLAAVAVAMGA
                     RRYAARHIDTVALMKCMGASQGFVLAISIIELSLLAVFAVALGALLGFLAQSGLAWLL
                     KDLIRTELPSASLAPLPIALVTVLAMLIGFALPPLLQLKSTPPARVLRKTVSAPPMRY
                     GLSYLLALAALTAILWSLVRDTELLLSLLAGVLAVGLVLTLAGFGLVRLTGRLRGGVG
                     VAWRYGLANVSRRGGGSVVQIVAFGLGLMVLLLLAVVRGDLLADWRRSLPANAPNNFL
                     INIRPEEREPLQQFLQAHAMGQPTMFPMVRARITAIDSRPADSIKLRGDSGRGFLERE
                     QNLTWSAGLMEDNQLIAGQWWTAADAGKPLVSISSEYQEALGLKLGDTLTFDVAGEPL
                     TVRVASIRKIRWDSFRPNFFLVLPPALLDGAGGTYMTSVFLTPAQRPALADLVRQFPT
                     ISVFDVDAILKQIREIMDRASLAVQYVFLFTLAAGVVVLLAAVQSTRDERRYESAILR
                     TLGASRGTVLQGVAAEFSALGFLSGTLAAFGATGIGWVLARRLFSLKFELDPWVWVVG
                     LVCGTVLVGVSGTLATRRVVNTPPIVTLRDD"
     gene            complement(13708..14454)
                     /locus_tag="VGE92_08555"
     CDS             complement(13708..14454)
                     /locus_tag="VGE92_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313915.1"
                     /translation="MNARTISPDNPPAAPPRFDRKFIEEHQLVERYLENKLPLKGARD
                     LENWCRANPDFLNELKLSERAQSSLKLLEACGRPVDLQEPQPPWWRNVYVLIGASIIA
                     LLSLLAFWAVLAKYMLLRGELEDTRALMNHGSLVQPATKTELRVSPDHVPGLEHARIL
                     VNRAAPQLMDLHVDLGYTAKLMQFRMFVDKKDQGRALVLNNLLKDSNGELRITLNSTG
                     LASGIYNVRIEALPFRGDPIPVGWLILEVR"
     gene            complement(14578..15900)
                     /locus_tag="VGE92_08560"
     CDS             complement(14578..15900)
                     /locus_tag="VGE92_08560"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY1313916.1"
                     /translation="MANSRAMRASARARLASNADWAFHASDAAVFEALATGRHAASLR
                     EYFGAPAYVELSALAAASKKAGRARGPYVLILPGIMGSKLGGRMPRAGTRKVSRRSQV
                     LWIDPLQIAAGRLTAMTLPSGIALEAMGVLLFSYAKLKLQLGLAGCDADFYSYDWRRG
                     VDELGAQLAARIAADGRPVILVAHSMGGLVARAAARILPKRLLRKLIMLGTPNHGSYA
                     PVQALRGTYPFVRNMSTLDRKHSAEHLAQHVFCTFPGLYHMLPRPGPKGVDLFDHRCW
                     PADGPAPSSQLLAAGAAAYAALAPADSRMVHIVGVNQETVVDLRRCAAGFEYFMDRNG
                     DGTVPLESARLPKLKCLFVDESHADLANNPRVIQAIIDLVRRGRTGALPQRWRARPGV
                     RRCISDAALLALGNRKIDWRHLTPAQREAVFAELDSGRLLPAARHPIA"
     gene            complement(15905..16804)
                     /locus_tag="VGE92_08565"
     CDS             complement(15905..16804)
                     /locus_tag="VGE92_08565"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY1313917.1"
                     /translation="MNLKDLKYLVALADTGHFGKAAERTFVSQPTLSAQLKKLEDYLG
                     VKLVERQPKNVQLTDVGKQVVVRARRMLSEGDEIVALARHNTDPFAGKLKLALIPTIG
                     PYLLPRVMPKLRKALPKLSLMLYEYQTESLLKRLRDGEIDVGIMALPAAQDGIESREL
                     YQEEFTVALPNGHPLSAKATIKVPDLKGHTLLLLEDGHCLRDQALEVCSRVDVREAED
                     FRATSLETLRQMVVAGLGITLLPELAVESPFGSQRGLAVRRFAKPAPSRSVGAVWRKS
                     STRIAVIAAVCDVVDRVMTAGKT"
     gene            16939..17502
                     /gene="ahpC"
                     /locus_tag="VGE92_08570"
     CDS             16939..17502
                     /gene="ahpC"
                     /locus_tag="VGE92_08570"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HEY1313918.1"
                     /translation="MSLINTQIKPFKATAFHNGKFVPVTHETLQGKWSVVVFYPADFT
                     FVCPTELGDLADNHAEFTKLGVEIYGVSTDTHFAHKAWHDTSDTIAKVRYPLVGDPTA
                     TLARNFGVLIEEEGLALRGTFVIDPAGLIKIMEVHDNGIGRDARELLRKVKAAQYVAA
                     HPGEVCPAKWTPGAHTLAPSLDLVGKI"
     gene            17510..19072
                     /gene="ahpF"
                     /locus_tag="VGE92_08575"
     CDS             17510..19072
                     /gene="ahpF"
                     /locus_tag="VGE92_08575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEY1313919.1"
                     /translation="MLDSNLKGQLQGYLARISQPVEIVAAVDDSDDSRAMQDLLADLQ
                     AASSLITVQVQRDAAALTPSFALRRPAGELQVRFAGVPMGHEFTSLVLALLQTGGYPP
                     KAERALLEQIRGLEGDFNFETVVSLSCQSCPDVVQALNLMAVLNPRVRHTMIDGALFQ
                     DEVERRQVMAVPAVFLNGRPFGQGRMELEEIVAKLDAGTAPRNAASLSAKAPFDVLVV
                     GGGPAGAAAAIYAARMGIRTGIAAERLGGQLLDTVGIENFISVKETQGHKLAAGLDEH
                     VATYDVDVMNRERAAALVPGDLIEVQLAGGAVLKARTVVIATGARWREMNVPGEREYR
                     NRGVAYCPHCDGPLFKGKPVAVIGGGNSGVEAAIDLAGLASHVWLIEFDAKLRADAIL
                     IAKLESLTNVTILTGAQTTEVTGDGARVHGLNYRDRRSGARHSLPLAGVFVQIGLVPN
                     TDWLQGTVKLSPRGEIEVDARGRTSVPGVFAAGDCTVVPYKQIVIAMGEGAKASLGAF
                     DHLIRAPLCAAA"
     gene            19088..20815
                     /locus_tag="VGE92_08580"
     CDS             19088..20815
                     /locus_tag="VGE92_08580"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEY1313920.1"
                     /translation="MLDIKRVTTIATGLAGVLIGLSLGTSALLRAATNDPPAYDLIIE
                     HGRVVDGTGAPWFEADVGVRAGRIAAIGRLDQASAKQRIDAAGRIVAPGFIDMLGQSE
                     LTLLVNPHVPSKIFQGITTEITGEGQSVAPVNAAIAQEAAPHFRHYRISQDWSDFAGY
                     FARLEKQGMGINIGSYVGATTVREMVIGFADRAATAEELTRMQGLVAASMRQGALGLS
                     SALEYAPAPYASTEELIALASTAAQFGGIYATHMRSEQEAIMASIDETIRIGREAHVA
                     VEIWHLKAGGVGNFGLMPEIVAHIGRARAAGVDIAADTYAYTAWYNEFSAFIPPWAHE
                     GGNAKLIARLRDPAVRARLKRELATPARDWDNEWQSVPGPEAILITTVLNPKLLSLQG
                     RNLAEIAKERGTDPMDTLFDILVEDGAQTSVAVFAMSESDVALAAIQPWVSFCNDSEG
                     TAPDGVLGSEFPHPRAYGTFPRVLRKYVREERRMPLEEAIRKFTSLPASRVRLADRGV
                     LKVGLWADIVVFDPERITDKATYSAPKQPSVGMQWVLINGVPVIADGVATNALPGRVL
                     RGQGYQPPH"
     gene            20939..21385
                     /locus_tag="VGE92_08585"
     CDS             20939..21385
                     /locus_tag="VGE92_08585"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY1313921.1"
                     /translation="MPANTPTRRLSSGVVVVSLAQRKLKFLLLRAYRNWDFPKGLVEA
                     GEEPIDAALREVREETTLDDLSFDWGLAYMDTGPYNKGKFSRYYIARSKETRVLLPVN
                     PDLGMPEHHEACWVDFNKALTMVSPRVQPVVRWAHAIINHEEMRKR"
     gene            complement(21382..22230)
                     /locus_tag="VGE92_08590"
     CDS             complement(21382..22230)
                     /locus_tag="VGE92_08590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEY1313922.1"
                     /translation="MPAHPFRIVNVFTFKRGVLSGNPLCVFERADDFDAGVMQALARQ
                     FNLSETTFILRSSRADARVRIFTPAYEMPFAGHPTLGTAHVCRALGLGGDCLRLEMPA
                     GVIAVSAQGDRWTLTGPAPTWREVDEPRGVLANALGLEERDIGDRPLWIKAGKEQLVV
                     PLRSAEAVRRAAPQPGAMRNIRSEDGIGMAYVFALNEAGTALARFFFPQGAAMLEDPA
                     TGSATVNLGGWCLAMQRALPLELEISQGEFVGRPSSLYLRVDAERQIFVGGDVLELGA
                     GSVHLD"
     gene            complement(22286..22444)
                     /locus_tag="VGE92_08595"
     CDS             complement(22286..22444)
                     /locus_tag="VGE92_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313923.1"
                     /translation="MKSLAKLLIPLSLIAAAMSGCVVAPAGYYHRGYYYGPRVAVVAP
                     APVVVVRP"
     gene            complement(22540..23265)
                     /locus_tag="VGE92_08600"
     CDS             complement(22540..23265)
                     /locus_tag="VGE92_08600"
                     /inference="COORDINATES: protein motif:HMM:NF009044.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY1313924.1"
                     /translation="MGRIFEVRKHAMFARWNRMAKQFTRVAKDIAMAVHAGGADPASN
                     PTLRRVLQNARAVNMPKDKTDAAIRRASGKEAANYQEILYEGYAPHGVALLVETATDN
                     PTRTVASVRNIFAKGAGNLGTTGSVSFLFKKMGVFRLDPAAVKDHDELELDLIDYGLE
                     EMGESTGDKGEPQLVIRGAFNDFGHLQEALEKRGITPLSAEHEYICAVPVELPEEQAA
                     EVLGLIDKLEQDDDVQKVFHSLA"
     gene            23332..>24317
                     /locus_tag="VGE92_08605"
     CDS             23332..>24317
                     /locus_tag="VGE92_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY1313925.1"
                     /translation="MSDATSFRDLELSEPVLRALSDVGYESPSPIQAATIPVLLSGAD
                     MLGQAQTGTGKTAAFALPALTRIDLSKHEPQVLVLVPTRELALQVAEAFLRYAAHLKG
                     FHVLPIYGGQSYQPQLNALRRGVHVVVGTPGRVIDHMNRGTLKLTGLTLLVLDEADEM
                     LRMGFIDAVESILEQTPPQRQVALFSATIPAPIRRIAAKHLRSPIEVTIKSKTSTATN
                     IRQRYWIVSGMHKLDALTRILEAEPFDGMLVFTRTKQSTVELAEKLEARGFAAAPLNG
                     DIAQPLRERTVARLKAGQIDILVATDVAARGLDVERIGHVVNFDVPHDPESYV"
BASE COUNT         4437 a         7776 c         7650 g         4454 t
ORIGIN      
        1 gttcaagaac gccgtgttgt cgctcaaggt gactccgctg atcacgcccg ataatcgcgt
       61 gattctggat ctggatgtgg ccgacgatac ggttggtgcg caggtgacga gcgccaccgg
      121 cggctccgtc ccctcgatcg acacccgcga gatcatcacc caggtgctgg tcaacgacgg
      181 ccagaccgtg gtgctgggcg gcattctcga taccaccaaa tcgaaatcgg ccaataaggt
      241 accgttcttt gcggatattc cgatattggg gaatctgttc aagagcacca tcaacatcaa
      301 caacaagact gaattgctga tcttcatcac gccaaagatc ctgcgtgaag gatcgaatct
      361 ctattaatcc cggatgtcga ctgcggtcga gcaaggatgg actaaatgcg cagatttttg
      421 actatagctt tgtgtttgac atgggtgctc gaaggctgcg gcggcggatc cggcgcgatc
      481 tccggtaaag gctcgaccac cggtaccacg ggcacaggca cctcgggcca gaccaccacc
      541 gtgctgtcgc tcggcagcgg caccggttca agcttccaac cgggcgtcat cgcgatctcg
      601 accaaatcac tctccgccgg cggcagcacc agcctgcaag tgtcgttggt cgatcagacc
      661 ggtgcggctt atacgaccgg gacccaaatc acgtttagtt cgccctgcgc cggtcaaggc
      721 ttggcgacca ttgcggcgac cggcgtaacg cccgcgagcc cgagcgtcac tacatccacc
      781 ggcacggcga gcgctaccta cgtggcaagc ggctgcagcg gcaacgatgt gataaccgcc
      841 gccgcaaccg caaacgacca atcgctcacg gcgaacggta cggtcacggt ggcgcaggcc
      901 gcgatcggct ctatctcatt catttccgca accccatcga gcattacgct caaaggggtc
      961 ggcagtgtcg gcggctcggc gacctcgact gtgatcttca aggttctgga cacttccggc
     1021 ggcccgcggg ccggagcgac ggtgaatttt tcactgaata ccacggtcgg cggcatcacg
     1081 atcgcgcctg cgtcggccac gaccgacgcc aacggacaag tgcaaaccat cgtcagcggc
     1141 ggaacggtgg ccacgaccgt ccgagtcacc gcgaccacga cggcgtcggg cggcggcacg
     1201 atttccacgg agtcgaatgc gttgacggtt tccacgggca ttcccacgag cgccaacatc
     1261 tcgctggcgg tgaagtgtca aaacgtcgaa gcctggaatg tcgacggggt cagcgtcccg
     1321 gtcacggtga gcatgacaga ccggttcagc aatccggtac ccgatggcac gaccgccaat
     1381 ttccaaacca cactcggcgg aattcaggcg agctgtcaga cgggctcagc cacggcagga
     1441 tcgggcgcat gcagcgttaa ttgggtcagc aaggcgccgc gaacggtcaa cggcaatcct
     1501 cagtccacca cgggcaactc caatacgtcc gccgcctatt gcagtacgcc ggtcggtgct
     1561 gcgctcggcg tgtgcggcgg caccaccaac ggccgcagcg cgattcttgt gaccgccatc
     1621 ggcgaagaaa gcttcaagga cgccaacggc agcggctttt tcaatccggc cgacacggtt
     1681 gcgtgggatg ccaccgatgg cgacaacaac ttttcgaacg gtcagccgaa accctggcaa
     1741 gacacgtcgg aaccgtttct taacgagtgg gagctttacg attcgttcgg tactccgagc
     1801 ttcgtgctcg gcgagccgtt cctcgacttc aacaataacg gcaagcgcga cggtccggac
     1861 ggactggtgg aaagtgcctt gtgcgagggt cctctgtgta atacgactgg ctcttcggtt
     1921 gccatcggtg ccaacaatct catcattttg tccggcagcc acgccaattc ctacgtagtc
     1981 agccccgcgg gtgggccgcc ctaccacttc aataacggtc tatcgctcgc cgtcgatatc
     2041 ttcgacgacc gaatgcagca aatgcccgca ggaaccacgg tggccgccgc cgtcggcagc
     2101 ggtgcgaccg cgagcatcac cagtccgctt ccgaccgcgg ggtggccatg ctcgtcggca
     2161 gctccttacg tcgatgggca agggaatctc atcgcgggcc agttattcaa ctttaccctg
     2221 gcgccggcta acccggctcc ggcgggcggg caagtgggcg gcgagctcta cctcaccatc
     2281 acgacacccg ctggtctcat caccacgatc gctatcccga tccaaccttg acctttccgc
     2341 gggcaagcga ggcgcgccat gcgcctcgct tgccccgccg cgcgcggctg ccacgatctc
     2401 gactatgatc gttgtcaact atgctcggca agggaaacat ctttctaatc ggtcccatgg
     2461 gttccggtaa aaccgccgtc gggcggcatt tggcgcgcat gctgcgctat acgtttcatg
     2521 atagcgacgc ggatatcgag gccagaaccg gcgtcgacat cgccttcatc ttcgagaaag
     2581 agggcgaaac cgggttcagg attcgcgaaa aggagtcgat tgcgcgtctc acgcttctcg
     2641 agtcaatcgt gctcgcaacc ggcggcggcg ccgtcatcga ggaggagaat cgccgcgtgc
     2701 tcgccgcgcg cggcgtggtg gtgtacctcg cgacctcgat cgaccaacaa ctcgagcgca
     2761 cgcgccatgc gcggcatcgc cccctgctca accacacgga cccggaacac acgctgcatg
     2821 aattgatgca gcgccgggcg cggctctatg ccgaaattgc cgatcttacc gtctcgaccg
     2881 acggccgccg ggtgcagctg gtggccgagg agattcatca tgagctcagg cgcgcgccgg
     2941 tcagctaaag gttcgtataa tcaacgccgt gcacactctc caggttgatg tcggtcattc
     3001 ccgctatccg atcgcgatcg gagcgggctt gcttggcgat cgcgagttgt tggcgggcag
     3061 cattcgcggc cacgatttgc tgatcgtgac caataccacg atcggcggcc tgtaccttgc
     3121 gaaactggcg gccgggctcg actcgcgcag gatcgccgaa tgcatcttgc cggaaggcga
     3181 gcagcacaag acgctgcata ccgcgggctt ggtattcgat gcgctggtgg cgaagaaaat
     3241 gaaccgcgac gccaccgtgc tcgccctggg cgggggcgtg atcggcgata tcgcaggctt
     3301 tgcggccgcc tgctatcagc gcggcgtcgg ctacgtgcag attccgacca cgctgctcgc
     3361 gcaagtcgac tcctcggtcg gcggcaagac cggcgtcaat catccgggcg gcaagaatct
     3421 gatcggcgcg ttctatcagc ccgaaagcgt catcgcggac atcgacaccc tcgcgacgct
     3481 gccggaccgc gaactctccg ccgggcttgc cgaggtcatc aaatacggct gcgtatggga
     3541 tccgttgctg ttcgattggc tggacaacaa catggcggcc ctcctggcgc gcgacgtcga
     3601 ggcccttgcc tacgccatcc gcaggtcttg cgaaatcaag gcgacggtcg tcgccaagga
     3661 tgaacgcgaa cagaatctgc gcgcgatctt gaacttcggc cacaccttcg gccatgccat
     3721 cgaagcggcc acgggctatg aaacctatct gcacggcgag gcggtggggc tcggaatgct
     3781 gattgcgtcc aacctctcgc accgcctggg gctgatcgat gcggccaccg aggcccgcat
     3841 cggggccatc ctggtaaggg ccggcctgcc cacccaggca ccgcgcgtcg gcgccgcgaa
     3901 agccttggag ctcatgcaga tggataagaa ggtgctggcg ggcgcactgc gcctggtgct
     3961 gatcgagaag ctcggacggg ccatcgtcac cggtgattac ccgcaaacgg ccctcaatgc
     4021 cacgctcgcg gaatatttcg cgtgagcgat ctcgccgcct atgccgccct cgacgagcac
     4081 tcgcgcgggc gccgccatcc cgagccgaaa cccgcttatc gctcggaata tcaacgcgac
     4141 cgcgatcgca tcgtgcattc gacggctttc cgccggctgg tctacaagac tcaggtcttc
     4201 gtcaaccacg agggcgacct gtatcgaacg cgcctgaccc attcgctgga ggtggcgcag
     4261 atcgcgcgca ccatcgcgcg cgctctcaag ctcaacgaag tgctgtccga ggccatttgc
     4321 ctcgcgcacg atctcggcca tacgccgttc ggccacgccg gacaggacgc cttgaacgag
     4381 tgcatgctgg atttcggcgg cttcgaacac aacctgcaat cgctgcgcgt ggtcgatgag
     4441 ctcgaggagc actatgccga attcgacggg ctgaacttga gttttgaaac ccgcgaagga
     4501 atcctcaagc attgctcgca cagcaatgcc ctgcagctcg gcgaattggg ccaacgcttc
     4561 atccatcgcc aacagccggg cctcgaggcg cagctggcaa acttcgccga cgaaattgcc
     4621 tataacaatc acgacgtgga ggacggaatc cgcgccggcc tcgtgagcgt cgagcaattg
     4681 ctggcggtgc cgctattccg cgagtaccac gccgcggtgc atgccaaata cccggcactt
     4741 gcgggccgtc gccaggtgta cgaaattctg cggcgcatga tcaatcatct ggtcacggat
     4801 ctgatcgact cgtccgcagc gcggctcgcc gccgcggcag tgcgctcgat cgaggaggtt
     4861 cgcgcgcatc cgcagccgct gatcggcttc agcgaccaga cgcgcgagct gaattacgcc
     4921 ctcaagagct tcctgcgcga gcatgtttac aggcactata aggtgcggcg catgaccgcc
     4981 aaggcgcagc gcgtgatccg cgagttgttc gacgcatttt tcggcgatcc tgggctgctg
     5041 cccgaggagc acaaagccac ggtttcgcgg ctggaaatgc tgcagggtgc ggcggggcgt
     5101 gcgcgcgcgg tggcggacta tatcgcgggc atgaccgatc gctacgcgat tctcgagcat
     5161 cgtcgcttgt tcgatccggg tgaacgcaca tgaatggaat acggtgaacg aaccactcaa
     5221 gaacgacttg ttgatacgcg cactgctgcg cgagccgacg ccgcgccggc cgatttggtt
     5281 gatgcggcag gcgggacggt acctgccgga ataccgcgcc acgcgcgcgc gcgccggcgg
     5341 atttctcgag atgtgcacca atcccgaaat cgcctgcgaa atcacgctgc agccggtcaa
     5401 ccgctatccg ttggacgcgg cgatcctgtt ctccgacatc ctgacgatcc cgcatgcgat
     5461 gaatttggga ttggaattcg aggccggcga aggccccaag ttcgagcgcc cggtgaggag
     5521 cgccgccgac atcgccaaac tgggagtgcc cgatcccggc cgtgaactca agtacgtgat
     5581 cgatgcggtg gcgttggtgc gccgcgagct gcacggcagg attccgctca tcggatttgc
     5641 gggcagccca tggacggtgg caacttacat ggtggaagga ggcggcagca agagcttcgg
     5701 ccagatcaag cgcatgatgt acgaagcgcc gcgcgatttg catcggctgc tcgcattgct
     5761 cgccaaggcg acggtgctgt atctgaatgc gcaaatcgcg gccggggcgc aggccgtcat
     5821 gctgttcgac acctggggcg gcgtgctcac acccgcgcag tatgaggaat tctcgctgcg
     5881 ttacatggcc gaggtggtcg atgcgctgac acgcaaggcc gagacgcggc gtgtgccgag
     5941 catcgtattt acgaagggcg gcggcgcttg gctcgacaag atcgcggcga tcggctgcga
     6001 tgcggtcggc gtcgactgga ccacggatct cgcggcggcg cgccgcgcgg tcgacgggcg
     6061 cgtggcatta cagggaaatc tggatccggc ggcgcttttc gccgcgccgg aaactttgcg
     6121 tgccgaaacc ttgaaggtgc tcgcgagcta cggcgcaggg gcgggacatg tgttcaacct
     6181 gggtcatggc attacgccgg acgtggaccc cgagcgggtc gccgtcctga tcgacaccgt
     6241 acagggctac ggcgtggttt gagcctgcgg cgtcgcgccg gccgcgcggc gcagctcgtc
     6301 gcgcagctcg cggcgcaaga tcttgccgac gttggatttg ggaagctcgc tgcggaagta
     6361 cacgtgcttc ggcaccttgt agcccgtcaa ttccttgcgg caatacgcga tcagcgcttc
     6421 ggccgtgaga ctcgggtctt tcttgacgac gaacagcgcg accacctcgc ctgaattctc
     6481 gtccggctgc gcgaccgcgg cgacttccat tacgcccggg agcgccgccg cgacgctttc
     6541 gatttcattc ggatacacat tgaagccgga caccagaatc atgtccttct tgcggtcctc
     6601 gatgaacacg aaaccggcgg cgttcatgcg cccgatatcg ccggtacgca gccaatcgcc
     6661 gaacatgact ttctcggtct cgtcaggccg gttccaatag ccgcgcatga cctgcggtcc
     6721 gaatacgcaa atctcggcga cggcgtccac cggcatttcc ttgccggcgt cgtcgcgaat
     6781 cgaaatgtcg gtcgagggga tgggcaggcc gatggaaccg ttgaaatcga gaccgatcgg
     6841 attggcggtg gcgatcggcg aggtctccgt caggccccag ccctgcgtga tgacgcagcc
     6901 cgtcacttcc ttccaacgct gcgcgaccac cgcctgcaat gccatgccgc cggcaaaggt
     6961 gacgcgcaag gcggaaaaat ccacgctttc gaatcccggc gtgtgcagca gcgcgttgaa
     7021 aagagtgttc acgccgctga tgaagttgaa cttgtgcttc ttcaattcgg cgacgaacgc
     7081 cttgaagtcg cgcggattgg tgatcaaaat gttgtgcgcg cccagggcga taaacgccag
     7141 gcaattggtg gtgagcgcga agatgtggta cagcggcagc gccgtcacga cgacctgcgg
     7201 ttcgccggcc gccaaggacg gtgtgatcca cgccgaggcc tgcagcacat tggcgaccat
     7261 gttgcgatgg gtgagagtcg cggccttggc aacgccggta gtgccgccgg tatattgcag
     7321 gaaagcgatg tcctccggcc ccaggggcag cgcttcgagt ttcaatccga ggccgccgcc
     7381 caaggcgctt ttgaacgtca tggcgcccgg cattttccac tcggggattt gcttgcgcac
     7441 gtacttcaat accagattca cgatcgtgcc gcgcggaact cccagcaagt cgccgatgcg
     7501 cgtgaccagc accttcttca attccgtatg cggcagcacc tgttgcagca catgcatgaa
     7561 gttctcgacg atgacgatgg ccttcgcacc gctgtcgacc aactgatgct cgagttcgcg
     7621 cgcggtatac aggggattgg tgttgacgac caccatgccg gcgcgcagca cgccgaacag
     7681 cgcgatcggg tactgcagga tattcggcat catgagagcg acgcggtcgc cgcgcaccag
     7741 gccggatttt tgctgcagcc acgccgcaaa ggcgcggctc agcgcgtcga gttgtgcgaa
     7801 gctcaagctt gcgcccaggt tgctgaacgc cggcgagtgg cgatgctccg cgcacgcttg
     7861 ctcgaaaatg tcccgcagcg atgcgtagct gtcgggatca atatcggtcg gcacgccggg
     7921 cgggtattgc gtcagccagt ttcgatccac tccgctgctc ctcgtttttt gttccgcgtg
     7981 tatagcatcc gtgatcgttg acgtaaactt gtggccatga atgccgcgcc cgacgcccgc
     8041 gaacagcaaa tcatccgctc gtggaacgtg aacgccgtgc cgtgggccga ggcgattcgg
     8101 tccgggagca tcgagagccg caagctcgtg accgatcgcg ccatcattga cgcggtgctc
     8161 gggcttggcc cgcgccgcat tctggatttg gggtgcggcg aaggttggct ggcgcgggcc
     8221 ttggccgctt gcgcaatcca gaccgtcggc atcgatgtcg tgccggaact catcgcggcg
     8281 gcggcgcgtc tcggcggcgg cgaatttctc gtgcgcgagt atcgcgaaat tgcgcaggct
     8341 cgctggcgcg cgcgcgagtt cgatgccgcc gtatgcaact tctccctgct tggcaaggag
     8401 tccgtggaat cgctgctggc tgcaatcccc gagtatctcg ccgcgcccgg atacctgatc
     8461 gtgcagacgc tgcatccact ggcagcgtgc ggcgatcttc cctacgagga tgcgtggcgc
     8521 gccggaagtt ggctcggatt cggtgcgcaa ttcagcgaac cggcaccctg gtatttccgc
     8581 accctcgaga gctggcattg catgctgcgg cggaccggat tcgaggtgct cgagtgtcgc
     8641 gaaccgacgg cgcccggtgc ccgggcgccg gcttccgtca ttttcatatg caagccgcgc
     8701 gtgcgagagc agactgctcg gacggcaccg gactcggtgc cggtctcgag tggcaattca
     8761 acacaatgag cggcgcttcc tcccaggcct aaaggccagg gtttgcgcgc cgaatattcg
     8821 atgaatctat ggcgtttgcg gcggattttg cgattggcgt cgagcggcgc gctgttggcg
     8881 ctctccggct gcgtcatcgg ctacggaccg tgcctgttgc agcagccggt gaagaataat
     8941 ctcaccggcc gggttcattt ccgcgactac ccgaccagcg aaggcatgga caaagttccg
     9001 gtgttggcgc tcgatcggac ggcctatgtg tacgcgcccg cacaaagccg tcattgcctg
     9061 cccgcaaacg acgtgcagct ggtgggcgtc gcggaattcc cgcaagacgt gatcgaaaat
     9121 tcgcatgtga gcgttgatgg caagcttttt caggcggggt cgtccagcca gcacacgcag
     9181 tttctgatga acgtaaccac catattgccc atcagtggcg cccgctgagc ccgggcgtct
     9241 acaatcgccc ggcaaactcg gattacacca gggagttgaa tcttatgaaa aatgcgaatt
     9301 tagcgggact tctcgccgcg cctttgatgc tgcttgcctt gtcgttgccc gctctggcgg
     9361 attcccccac ccccacgccc acgacctgca aggacggcac tacctccacg gccaccggta
     9421 agggtgcctg cagcggtcat ggcggcgtgc aaaaggccgc tgcgactgcc accgccccgg
     9481 ctgccgctgc gccggcaatg cctgcgggcg gcgcgcctgc tgcctcggga gccaccgcca
     9541 cttgcgagga tggcaccaag tccacggcga ccggcaaggg tgcctgcagc ggtcacggcg
     9601 gcgtgctgaa accctcgaag agcgcggcgc ctgccgcagc accagcgccc ggcactgcca
     9661 tgacagcggc tcccgctgct gccgccaaga cctcgacggc gaccaagtcg gcaccggcca
     9721 gcagcacgga tccgaccggt gcaaccgcca agtgcaagga cgggacctat tcgaagtcga
     9781 cgcacaaggc cgggacctgt tcgagtcacg gcggcgttgc ggagtggctg accgccgctc
     9841 agtaacggca acgacgaggc cgggcggcgg cgtccgcccg gcccatcgcc gccgccgcga
     9901 gtcacgcctc taacggcgca gcaacgggag cagcgacggc cacacattgg ccagcaattt
     9961 gggttgcccg agttcgttcg gatgtacccc gtccgcttgc atgagacttg gcgacagggc
    10021 gatgtcgttg agcagaaatg gcaccaacgg caatttcttg tcgcgggcca cctcgctgta
    10081 taccagccga aatcgctccg tgtaatcggg cccgtagttc gggggcatgc gcattccgag
    10141 caataacact cgagctcccg ccgcggtggc cagatcgacc atgcgcacca aattagcgcg
    10201 catcggcgca accggcaggg cgcgcaagcc atcgttgccg cccaattcca gcacgacaat
    10261 ggccgggtga tgcaggtcga gcgcccgtgg caggcgcgcc aggccgccgg cggtggtctc
    10321 gccgctaacg ctggcgttga tcacctggta accgtactct tgttccttga gcctcgtctg
    10381 cagcaacgcc acccagccct gttctacgcg cgccaagccg tagccggcac tgatgctatc
    10441 gccgaacacg agaatcaccg gcgtttcggc ccgggccggg acggacgcag caatcaacac
    10501 ccacaggatc aacagccgtt tcacaggcgc tcctcaatgc aagatcaaat cgttctcgaa
    10561 gcgaagaatg tgtccaagac cgtcgccagc cccgaaggcc agctcaccat tctcgccgat
    10621 gtcagcctca gtgtgcgaac cggcgagacg ctcgccatcg tcggcgcttc cggcgccggc
    10681 aagtcgacgc tgttggcctt gctcgcgggg ctcgactcgc ctagcaccgg ccgcgtactg
    10741 attgccggcg tcgatttgac ccagctcgac gaagacggcc gggcggcgat tcgcggcaag
    10801 catgtggggt tcgtatttca gtcctttcat ttgattccgt cgctgaccgc ggtcgagaac
    10861 gtcatgctgc cgctcgagct aggccagagc cgcgatgcgc ggcaggtggc gagcgccgcg
    10921 ctcgcccaag tcgggctgac gccgcggctc gcgcattatc ccaagcagtt gtcgggcggc
    10981 gaacagcagc gcgtggcgat cgcgcgcgcc ttcgtcaccc atccagcggt gttgtttgcc
    11041 gatgagccga cgggcaattt ggacacggca accgggcaac gcatcaccga tcttctattc
    11101 gatctgaatc gcagcaccgg cagcacgctg gtgctggtca ctcacgaccg cgaattggcc
    11161 gcacgctgcg gccgggtctt ggaacttgac gccggccata cggtgaattg aatgcaagtc
    11221 ctgcgcttcg cattacgcaa cttgtggcgc gatctcaaat ccggcgagct gtcggtgctg
    11281 ctgctggcgc tgaccgtggc cgtgttgtcc ttgacggcgg tcggcttctt caccagcaga
    11341 atctcgcagg gagtgcgtgc tcaggccggc gaggtgctag ccgccgatct gcgtctggaa
    11401 tccccgcgcc cgatcgcgcc gcgctacttc gccgaggcgc agcgccgcgg cttgcgaacc
    11461 gcgagcatac tgtcctttcc gaccgcgata ttcagcggcg atgtgagtca actggcggcg
    11521 ctcaatgcgg tgaccgcggg ctacccgttg cgcggtcgct tgcgcatcgc ggacgcgcct
    11581 ttcggcgcgg cacgcgcgac ggatcgcata cccggccgcg gccaagtctg gatcgatgcg
    11641 cgcatcatcg cccagttgaa aatcgagctc ggagcgacgc tcaagatcgg tgcggcgtcg
    11701 tttcgcgtga cgcaagtgct cgactaccgg cccgaccagg gcacgggttt cgtcaatctc
    11761 gcgcccgccg cgctgctcaa ttacgacgct gtggcatcga ctcagctgat ccagcccggc
    11821 agccgggtga cctacgcggc gctgttcgca ggtcccgcgc aagcggtgag cgacttccat
    11881 gaatacctgg tatcgaccaa ggcgccgggc gagcgtctgc gcgagatcga cgaatcgagc
    11941 cgtcaattga attccgcaat cgatcgatcc agccgcttcc tcaatttggc gagtctcgca
    12001 tcggtgctgc tcgccgcggt cgcggtcgcg atgggcgcgc gacgctatgc ggcgcggcac
    12061 atcgacacgg tggcgctgat gaagtgcatg ggggcgtcgc agggcttcgt actggcgata
    12121 tcgatcatcg aactgagctt attggcggtg ttcgccgtgg cgctcggcgc actgctcggc
    12181 ttcctggcgc aatcgggtct cgcgtggctg ctcaaggatc tcattcgcac cgaactgccg
    12241 tcggcctcct tggcgccgct gccgatcgcg ttggtcacgg tgttggccat gctcatcggg
    12301 ttcgccctgc cgccgctgtt gcagttgaaa agcacgccgc ccgcccgggt gctgcgcaag
    12361 accgtgagcg cgccgcccat gcgctatgga ttgagttatc tgttggccct ggcggcactg
    12421 accgccattc tgtggagctt ggtacgcgat accgagttgt tgctgtcgct gctcgcgggc
    12481 gtgctggctg tcggattggt gttgaccctg gcggggtttg gtttggtacg gctgacgggc
    12541 cggctgcgtg gcggagtcgg ggtcgcttgg cgctatggtc tcgccaacgt atcgcggcgc
    12601 ggcggcggca gcgtggtgca aatcgtcgct ttcggcttgg gattgatggt gctgctgctg
    12661 ctcgcggtgg tgcgcggcga tctgttggcc gactggcggc gcagtttgcc ggccaatgcg
    12721 ccgaataatt tcctgatcaa catccgcccg gaagagcgcg agccgctgca gcaattcttg
    12781 caggcacacg cgatggggca gccgaccatg tttcccatgg tgcgagcgcg cattaccgcg
    12841 atcgactccc ggcccgccga ctccatcaag ttgcgcggcg acagcggccg cggctttctc
    12901 gagcgcgagc agaatctcac ctggtctgcc ggcttgatgg aagacaatca actcatcgcc
    12961 ggacagtggt ggaccgcggc cgacgccggc aaacccttgg tatccatctc ctccgaatac
    13021 caggaagccc tgggtctgaa gctcggcgat acgctcacct tcgacgtcgc cggagaaccg
    13081 ttgacggtac gggtcgccag tatccgcaag attcgctggg acagctttcg cccgaacttc
    13141 tttctggtgc ttccgccggc actgctcgac ggcgccgggg gcacttacat gaccagcgtg
    13201 tttctgaccc cggcgcagcg gcccgccttg gcggatctgg tgcggcagtt cccgacgatc
    13261 tcggttttcg atgtcgatgc cattctcaag cagatccgcg aaatcatgga tcgtgcatcg
    13321 ctcgccgtgc agtacgtctt cctgttcact ctggcggcgg gcgtcgtggt gctgctcgcg
    13381 gcggtgcagt ccacccgcga cgagcgccgc tacgagagcg ccattctgcg caccctgggc
    13441 gcgagccgcg gcaccgtgct gcagggcgtg gccgccgaat tctcggcgtt ggggtttcta
    13501 tcgggaactc ttgcggcctt tggcgccacg ggtatcggct gggttttggc acggcggttg
    13561 ttttcgctca agttcgaact cgatccgtgg gtatgggtcg tgggactggt gtgcggcacg
    13621 gtactcgtcg gcgtgagcgg cacgctggcc acgcgccgag tcgtcaatac gccgcccatc
    13681 gtcacgctgc gcgacgactg agtcgcttca acgaacttcg aggatcagcc agcccaccgg
    13741 aatcggatcg ccgcgaaacg gcagcgcctc gatccgcacg ttgtagatgc cggacgcaag
    13801 ccccgtggaa ttgagcgtga tgcgcagttc accgttggaa tccttgagca gattgttcaa
    13861 gaccaaggcg cggccctgat ccttcttgtc gacgaacatg cgaaattgca tcagcttggc
    13921 ggtgtagccc agatcgacgt gcagatccat cagctgcggc gcggcgcggt tcaccaggat
    13981 ccgcgcatgc tcgagaccgg gaacgtgatc cgggctcacg cgcaattccg tcttggtcgc
    14041 cggctgcacc agcgaaccat gattcatcaa cgcccgcgtg tcttcgagct cgccgcgcaa
    14101 caacatgtac tttgcgagca ccgcccagaa cgcgagcaga ctcagcagag cgatgatgct
    14161 cgcgccgatg agaacataga cgtttctcca ccagggtggt tgcggctcct gcagatcgac
    14221 cggcctgccg cacgcttcga gcagcttgag gctcgattgg gcgcgctcgg agagcttcag
    14281 ttcgttcaaa aagtcgggat tcgcgcggca ccaattctcg agatcgcgcg cgcccttcaa
    14341 tggcagcttg ttctcgagat agcgctcgac gagctggtgc tcctcgatga acttgcgatc
    14401 gaagcgcggc ggcgcggccg gcggattatc cggtgaaatg gtgcgagcgt tcatgccttt
    14461 cagttcctgc ttggcatcat gccgcctctg cgccgcagaa tatgtgaaat gatcggcata
    14521 tgaccggcgc aggctccccc tggggtgtgg gacgagacgc gcgtcactat tcctcgctca
    14581 ggcgattgga tgtcgcgcgg cgggcagtag gcggccgcta tccagttccg cgaatacggc
    14641 ctcgcgctgc gccggggtga ggtgccgcca atcgatcttg cggttgccta gggcgagaag
    14701 ggccgcatcg gagatgcatc gcctgactcc cggccgggcc cgccagcgct gcggcagtgc
    14761 gccggtgcgt ccgcggcgca ccaaatcgat gatcgcttga atcacgcgtg ggttgttggc
    14821 gagatcggcg tgcgactcat cgacgaacag gcacttgagc ttcggcagcc gcgccgattc
    14881 gagcggcacc gtcccgtcgc cgttgcggtc catgaagtat tcgaaaccgg cggcgcagcg
    14941 gcgcaggtca accacggttt cctggttgac gccgacaatg tgcaccatgc gcgagtcagc
    15001 gggcgccaac gccgcgtaag cggccgcgcc ggcggccaga agctgcgaac tcggggcggg
    15061 gccgtcggct ggccagcagc gatggtcgaa caagtccact cccttgggcc ccggccgcgg
    15121 cagcatgtga tacaggcccg gaaaggtgca gaacacgtgt tgcgccaagt gttcggccga
    15181 gtgcttgcgg tcgagggtcg acatattgcg cacgaacgga tacgtgccgc gcaacgcctg
    15241 caccggcgcg tacgagccat gattgggagt tcccagcatg atgagcttgc gcagcagccg
    15301 cttcggcaag atccgcgcgg cggcgcgcgc gaccaggccg cccatgctgt gggcgaccag
    15361 aatgaccggc cgtccatccg ccgcaatccg tgccgcgagc tgcgccccga gctcatcgac
    15421 gccgcgccgc caatcgtagg aataaaaatc cgcatcacaa cctgcgagtc cgagctgcag
    15481 cttgagtttc gcgtaagaga acagcaatac gcccatcgct tcgagcgcta tacccgaggg
    15541 cagcgtcatc gcggtcaaac ggcccgccgc gatttgcaac ggatcgatcc acaacacctg
    15601 agatcgacgg ctgaccttgc gcgtgcccgc gcgcggcatc cggccgccga gcttcgagcc
    15661 catgattccc ggcaggatca acacgtaagg accgcgcgcg cggccggcct tcttgctcgc
    15721 cgccgcaagt gccgacagtt ccacgtatgc gggagcaccg aagtattcgc gcaggctcgc
    15781 tgcgtggcgg ccggtggcga gcgcctcaaa aacggccgca tcgcttgcgt gaaacgccca
    15841 atccgcgttg ctggccaaac gcgccctggc cgaagcgcgc atcgctcggg agttcgccat
    15901 gggcttaggt cttgcccgcc gtcatgacac gatccacgac gtcgcacacc gccgcgatga
    15961 ccgcgatgcg cgtgctcgac ttgcgccaca cggcgccgac gctgcgcgaa ggcgcgggct
    16021 tcgcgaagcg gcgcacggcg agaccccgct gcgatccaaa cggcgactcg acggcgagtt
    16081 ccggcagcag cgtgatgccg agaccggcga ccaccatttg ccgcagggtt tccaggctgg
    16141 tcgcgcgaaa atcctcggct tcgcgcacgt cgacccggct gcaaacctcc aaggcctgat
    16201 cgcgcaagca atgcccatcc tccaacagca gcaaggtgtg gcctttcaag tccggcactt
    16261 tgatggtcgc tttggcactc aagggatgcc cgttcggcaa cgccaccgtg aactcctcct
    16321 ggtagagttc gcgcgattcg atgccgtcct gcgcggcggg aagcgccatg atgccgacat
    16381 cgatctcgcc gtcacgcagc cgcttcaaca aagattcggt ttggtactcg tacagcatca
    16441 ggctcagttt cggcagcgcc ttgcgcagct tcggcatcac ccgcggcaat aaataaggac
    16501 cgatggtcgg aatcagggcc agcttgagct tcccggcaaa cgggtcggtg ttgtggcggg
    16561 ccagggcgac gatttcgtcg ccctcgctca acatgcggcg cgcgcgtacc acgacttgct
    16621 tgccgacatc cgtgagctgc acgttcttcg gctggcgctc caccagtttg accccgaggt
    16681 agtcctcgag cttcttcagc tgcgccgaca aggtcggctg gctgacgaag gtgcgttccg
    16741 cagccttgcc gaaatggccg gtatccgcca gcgccaccaa gtatttaaga tccttgagat
    16801 tcatcgcatg gtcccaatta aagcgttacg gcaatagacg tactctatca aaatcatcgt
    16861 tattatccaa ttctataatg ggtgcgccgc tgctaggatg tgcttcgttg cgtaataact
    16921 tgctcgagga tcgattctat gtctctgatc aatactcaaa taaaaccttt caaggcgacc
    16981 gcttttcaca acggcaaatt cgtgccggtg acgcacgaga cgttgcaagg aaaatggtcc
    17041 gtggtggtgt tctatcccgc ggatttcacc ttcgtctgtc cgacggagct cggcgatctc
    17101 gccgacaacc acgcggaatt caccaagctg ggcgtcgaaa tttacggcgt ctccaccgac
    17161 acgcacttcg cgcacaaggc ttggcatgac acctccgata ccatcgccaa ggtgaggtat
    17221 ccgttggtcg gcgatccgac cgcgaccctg gcgcgtaatt tcggcgtcct gatcgaggag
    17281 gaaggacttg cgttgcgcgg caccttcgtc atcgacccgg cgggattgat caagatcatg
    17341 gaagttcacg acaatgggat cggccgcgat gcccgcgagc tgctgcgcaa ggtcaaggcc
    17401 gcgcagtacg tcgcagcgca cccgggcgag gtatgcccgg cgaaatggac tccgggcgca
    17461 cacacgctgg cgccgtcctt ggatttggtc ggcaagatct gagcacgaca tgctcgacag
    17521 caacctcaag gggcaattgc agggctatct tgcgcgaatt tcacagccgg tggaaatagt
    17581 cgctgccgtc gacgacagcg acgactcgcg tgcgatgcag gatctgttgg cggacttgca
    17641 ggccgcctcg tcgctgatca cggtgcaggt gcaacgcgac gctgccgcgc tcactccctc
    17701 atttgcactg cgccggccgg cgggcgaatt gcaggtgcgg ttcgccgggg tgccgatggg
    17761 gcacgagttt acttccctgg tgctcgcgct gctgcagacc ggcggctatc cgccgaaagc
    17821 cgagcgcgcg ctgctcgagc agatccgtgg gctcgagggc gacttcaatt tcgagactgt
    17881 cgtttcactc agctgccaga gctgtcccga cgtggtgcag gcgttgaacc tcatggcggt
    17941 gctgaatccg cgcgtgcgcc acacgatgat cgacggcgcg ctgtttcaag acgaagtcga
    18001 gcggcgccag gtcatggcgg tgccggcggt gttcctcaac ggccgtccct tcggccaggg
    18061 ccgcatggaa ctcgaggaaa tcgttgcgaa gctcgacgcg ggcacggcgc cgcgtaatgc
    18121 ggcgagcttg agtgccaagg cgccgttcga tgtcctggtt gtcggcggcg gtccggcggg
    18181 cgcggcggcg gccatctatg ccgcacgcat gggcatacgc accggcatcg cagccgagcg
    18241 gctcggcggc cagttgctcg acaccgtcgg catcgaaaac ttcatttccg tgaaagagac
    18301 ccaaggtcac aagctggccg cgggcctcga tgagcacgtc gccacctatg acgtggatgt
    18361 gatgaaccgc gagcgcgcgg ccgcgctcgt tcccggggat ctgatcgaag tgcagctcgc
    18421 cggcggcgcc gtgctcaagg cgcgcaccgt ggtcattgcg accggcgcac gctggcggga
    18481 aatgaatgtg ccgggtgagc gcgaataccg caaccgcggc gtcgcctatt gtccgcattg
    18541 cgatggaccg ctgttcaagg gtaaaccggt cgccgtcatc ggcggcggca actcgggcgt
    18601 cgaggcggcg atcgatctcg ccggactcgc gagccacgtc tggctgatcg aattcgacgc
    18661 caagctgcgc gccgacgcaa tcctcatcgc caaactcgag agcttgacca atgtaaccat
    18721 cctcacggga gcccagacca ccgaagtaac cggcgacggc gcgcgcgtcc acggattgaa
    18781 ttaccgcgat cgacgcagcg gtgcccggca ttcgctgccg ctcgccggtg tcttcgtgca
    18841 aatcggcctg gtgccgaaca ccgactggct gcaaggaacc gtgaagctct cgccgcgcgg
    18901 cgagatcgag gtggacgcgc gcggccgcac atcggtgccc ggtgtatttg cagcgggcga
    18961 ttgcaccgtc gtaccctaca agcaaatcgt gattgcgatg ggcgaaggtg caaaggcctc
    19021 gctcggcgcc ttcgatcatc tgatccgtgc gccgctctgc gcggccgcct agggaaacaa
    19081 acccgccatg ctggacatca agcgcgtaac aaccattgcc acggggctcg ctggcgtttt
    19141 aatcgggctt tcactcggta cgagcgcctt gctgcgcgcc gcgacgaacg acccgcccgc
    19201 ctacgatctt atcatcgagc atggccgcgt cgtcgatggt acaggtgcac cctggttcga
    19261 agccgatgtc ggtgtgcgcg cgggacggat cgctgcgatc ggcaggctcg atcaagcatc
    19321 ggcgaaacag cgcatcgacg cggccggacg aatcgtcgca cccgggttca tcgacatgct
    19381 gggccaatcc gaacttaccc tgctggtaaa tccgcatgtt ccctccaaga tctttcaggg
    19441 aatcaccacg gaaatcacgg gtgaaggcca atccgtcgcg cctgtcaatg cagccatcgc
    19501 gcaggaggcc gcaccgcatt tccgccacta ccgaatctcc caggactgga gcgacttcgc
    19561 ggggtatttt gcccgcctcg agaagcaggg gatgggcatc aatatcgggt cctacgtcgg
    19621 cgcaaccacg gttcgtgaga tggtcatagg atttgcagac agggcggcga cagccgagga
    19681 actgactcga atgcaggggc tcgtcgccgc atccatgcgt caaggtgccc tcggactatc
    19741 gagtgcgttg gagtatgccc cggcgccgta cgcgagcacg gaggaactga tcgcccttgc
    19801 cagcacggcg gctcagttcg gcggcatcta cgcaactcac atgcgctcgg aacaggaagc
    19861 catcatggcc tcgatcgatg aaacgatccg aatcggccgt gaggctcatg ttgcagtcga
    19921 gatctggcat ctgaaggccg gtggcgtcgg taatttcggc ctgatgccgg agatcgtcgc
    19981 gcacattggc cgagctcgcg cggcgggcgt ggacatagcg gcggacactt acgcctacac
    20041 cgcatggtac aacgagttct cggcgttcat tcctccctgg gcacatgagg gcggcaacgc
    20101 caagctgatt gcgcggttac gggaccccgc tgtacgcgca cgcctcaagc gggagctcgc
    20161 aactccggcg cgtgactggg acaacgaatg gcagtcggtg cccggcccgg aagcgatact
    20221 catcaccact gttctcaatc cgaagctgct cagcctgcag ggtcgcaacc tcgcggagat
    20281 tgccaaagag cgcggcaccg atcccatgga cacgctgttc gacattctcg tcgaggacgg
    20341 ggcgcaaacc tccgtggcag ttttcgccat gagcgagtcc gacgtggcgc ttgcggcgat
    20401 acagccgtgg gtatcgttct gcaacgatag cgaaggcacc gcgcctgacg gggtgctcgg
    20461 ctccgagttt ccgcatcccc gcgcttatgg aaccttcccg cgcgtacttc gcaaatatgt
    20521 gcgcgaggag cgccggatgc cgctcgagga ggctatccgc aaattcacca gccttccggc
    20581 ctcgcgtgtg cgtctcgcag atcgcggcgt actcaaggta ggattatggg ccgatatcgt
    20641 cgtgttcgat cctgaacgga tcaccgacaa ggcgacctac agcgcgccca agcagccttc
    20701 ggtcggcatg caatgggtat tgatcaatgg ggtgcctgtc atcgccgatg gcgtggcgac
    20761 gaatgcactg cctggccggg tcctgcgagg gcagggctat cagccaccgc attgacgagt
    20821 catctgcgaa tcctcggcag agttcgcatc gcttgcactc tagaaaatcg tcgccgctgc
    20881 atgggtatac tcctgcgggg gccggccttg gcgcgggcat tcccctagga gcgtaccgat
    20941 gcctgcgaac acccccactc gtcgactgtc ttccggcgtc gtggtggtga gcttggcgca
    21001 gcgaaagctc aagtttctgc tgttgcgcgc ctatcgcaat tgggatttcc cgaaggggct
    21061 ggtcgaagcg ggcgaggaac cgatcgacgc ggcgctgcgc gaagtgcgcg aagagaccac
    21121 gctcgatgac ctatccttcg attggggcct cgcgtacatg gacacgggcc cgtacaacaa
    21181 gggcaagttc tcccgctact acatcgcccg cagcaaggag acccgcgtgc tgttgccggt
    21241 caatcccgat ctcggtatgc cggagcatca cgaagcctgt tgggtggatt tcaacaaggc
    21301 gctgacgatg gtgtcgccgc gcgtgcagcc ggtggtgcgt tgggcacatg ccatcatcaa
    21361 tcacgaagag atgcgcaagc gctagtcgag gtgcacgctc ccggcgccga gttcgaggac
    21421 atcgccgccg acgaagatct gccgttcggc atccacccgc aaatagagcg acgatgggcg
    21481 gcctacgaat tcgccctgcg agatctcaag ctcgagcggc agtgctcttt gcatcgccag
    21541 gcaccagccg ccgagattga cggtggcgga ccccgttgcg ggatcctcga gcatggcggc
    21601 accctgcggg aaaaagaacc gcgccagcgc cgtgcccgcc tcgttcaacg cgaatacata
    21661 ggccatgccg ataccatcct cgctgcggat attgcgcatt gcgccgggtt gaggcgccgc
    21721 ccgccgcacg gcctcggcgc tgcgcagcgg cacgaccagt tgttcctttc cggccttgat
    21781 ccacaatggc cgatcgccga tatcgcgttc ttcgaggccg agcgcgttcg cgagcacgcc
    21841 gcgcggctca tcgacctcgc gccaggtcgg cgccgggccc gtcagtgtcc agcgatcgcc
    21901 ctgcgcgctg accgctatca cgcccgccgg catttccaaa cgcaagcaat ccccgcccaa
    21961 cccaagcgcg cggcacacat gcgccgtgcc gagagtcggg tggccggcga acggcatctc
    22021 ataggcggga gtgaaaatcc tgactcgcgc atcggcccgc gaggagcgca ggatgaacgt
    22081 ggtttccgag aggttgaatt gccgggcgag cgcctgcatg accccggcgt cgaaatcgtc
    22141 cgcgcgctcg aacacgcaca gcggattgcc gctcagcaca ccgcgcttga aggtgaagac
    22201 attgacgatg cgaaacgggt gagccggcat actttcaagt attgcacgac ccacccattg
    22261 gcgacgctgg catgcatgcg agcgactacg gtcgaaccac tacgaccggc gccggggcga
    22321 ccaccgccac ccgcggaccg tagtaatacc cgcggtgata atagcccgcc ggcgcgacca
    22381 cgcagcccga catggccgcc gcaatcagac tcaatggaat caataactta gctagacttt
    22441 tcataacccg ctcctcaaag ttttgcgcct cagatgagaa caacgcagcg ccgcagcagg
    22501 ggatgacagg atgaacaatg cttaaggcgg cggctccgct cacgccaggg agtggaatac
    22561 cttctgcacg tcgtcgtctt gttcgagttt gtcgatcaaa cccaggacct cggctgcctg
    22621 ctcttcgggc agctcgaccg gcaccgcgca aatgtactca tgctcggcgg agagcggcgt
    22681 gatgccgcgc ttttcgagcg cttcttgtaa atggccgaag tcattgaacg cgccgcgaat
    22741 caccaattgc ggctcgccct tgtcgccggt gctctcgccc atttcttcga gtccgtaatc
    22801 gatcaaatcg agctcgagtt catcgtggtc cttgacggcg gcgggatcca gacgaaacac
    22861 gcccattttc ttgaacagga aactgacgct gccggtcgta ccgagattgc cggccccctt
    22921 tgcaaagata ttgcgcacgc tggcaacggt ccgggtcgga ttgtccgtgg cggtctcgac
    22981 caacagagcg acgccatgcg gcgcatagcc ttcgtaaagg atttcctgat aattggcggc
    23041 ttccttgccg gaggcgcggc gaatggcggc gtcggtcttg tccttcggca tgttgacggc
    23101 gcgggcattc tgcagcacgc gccgcagcgt tgggttcgac gcggggtcgg caccgcccgc
    23161 gtggaccgcc atggcgatgt ccttggcaac gcgcgtgaac tgctttgcca tccgattcca
    23221 acgcgcgaac atcgcgtgct ttctgacttc gaatatgcgt cccatgccgc ctatggtagc
    23281 aatactcgtt caaaccgcta gacttgctgc tacctcttta aggcgattcc aatgagcgac
    23341 gcgacgagct tccgtgacct cgaactgtcc gagcccgttc tgcgcgcgtt gagcgatgtc
    23401 ggctacgagt ctccttcgcc gattcaggct gccacgattc cggtgctgtt gtcgggagcc
    23461 gacatgctcg gccaggcgca aaccggcacc ggcaaaaccg cggcctttgc cctgccggcg
    23521 ttgacgcgca tcgatctgtc gaagcacgag ccgcaggttc tggtgctggt cccgacgcgc
    23581 gaactcgcgc tgcaggtggc cgaggcgttc ttgcgttacg ccgcccacct caaggggttt
    23641 cacgtactgc cgatctacgg cggccagagc taccaaccgc agttgaatgc cctgcggcgc
    23701 ggcgtgcacg tggtcgtcgg cacgccgggg cgggtcatcg accacatgaa tcgcggcacc
    23761 ctgaaactca ccgggttgac cttgttggtc ctcgacgagg ccgatgagat gctgcgcatg
    23821 ggcttcatcg atgccgtcga aagcatcctc gaacagacgc cgccgcaacg ccaggtggcc
    23881 ctgttttcgg ccaccatccc tgcgccgatc cgccgcatcg ccgccaagca tctacgctcg
    23941 ccgatcgaag tcaccatcaa gagcaagacc agcacggcca ccaatatccg ccaacgctat
    24001 tggatagtca gcggcatgca caagctcgac gcgctgacgc ggatactcga agcggagccg
    24061 ttcgacggaa tgttggtatt cacccgcacc aagcagtcga cggtcgaact tgccgaaaaa
    24121 ctcgaagcgc gcggctttgc ggccgcgccg ctcaacggcg acatcgcgca accgctgcgc
    24181 gaacgcaccg tggcgcggct caaggccggc cagatcgaca tcctggtcgc gaccgacgtc
    24241 gcggcgcgcg ggctcgatgt cgagcgcatc gggcacgtgg tgaatttcga cgtgccgcac
    24301 gacccggaat cctacgt
//
LOCUS       DASWZT010000313         6032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_55661, whole genome shotgun sequence.
ACCESSION   DASWZT010000313 DASWZT010000000
VERSION     DASWZT010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6032
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55661"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VGE92_08610"
     CDS             complement(<1..769)
                     /locus_tag="VGE92_08610"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY1313926.1"
                     /translation="MRKSSKKTAKPARIRRARDAHSRQRQRLIDACISALHLYGPSRT
                     TVEKVTAFAKMSPGIVRFYFASKAAMLVASLQFLSAEFEEQLLVPVARLKSRPVEALE
                     LMVDLYLDPDIASPRKVSVWYAFWGEASSRQEYYDICGQKDESFAALVRELIERLIEA
                     TSRPELDPDGIALGLIGVLEMLWQDFAFRSEAEIDRAAAKRRAMAYLRSIFPGQFAQA
                     PPVGSVQLDNRLPGWVYADARLFAVEREALFRGAWQMA"
     gene            788..1744
                     /locus_tag="VGE92_08615"
     CDS             788..1744
                     /locus_tag="VGE92_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY1313927.1"
                     /translation="MTTATHDPIMHAPLRLPPRRPGIEWLQRQGKWLIILPPLLFLLV
                     FFLIPFGFALVISFADTAVRVPPFSDILSVSRDWHINLTLNLGNFKYLFSDEVYGLSY
                     IYSLKTAFFSTLICLALGYPMAYAIARTSKSTQSILLLVIILPFWTSFLLRVYALEGI
                     IRETGLLNTALLWLGIIHQPLQIMRTTLAVYLGIIYSYLPFMVLPLFATLEKLDHSLL
                     EAAGDLGSPPWRAFLEVTLPLSMPGIIAGSMLVFIPAIGEFVIPTLLGGPDNLMIGRV
                     LWDEFFGNRDWPVASAVAIAFLVLLVGPIALYQHYNSKQLEA"
     gene            1746..2576
                     /locus_tag="VGE92_08620"
     CDS             1746..2576
                     /locus_tag="VGE92_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY1313928.1"
                     /translation="MENRISPFLVSILCIGIAILYIPILVLIGYSFNASSLVSVWGGF
                     STSWYGELLHNDQILDAAFLSLKIGVVVSTGAVILGTLAAISLVRFKFFRGRLLLTGM
                     VNAPLVMPEIITGITQLLLFVSMLHLLGWPHRGFTTIVIAHIAFCSAYVTVTVQSRLA
                     SADRSLEEAAMDLGSGPVRAFLDTTLPSIAPALASSWLLSFTLSLDDLVISSFVSGPG
                     ASTLPMVIFSKVKLGVSPDVNALASLMICLVGACVLFAGYLMRRAERQRVLDAQMAER
                     "
     gene            complement(2585..3193)
                     /locus_tag="VGE92_08625"
     CDS             complement(2585..3193)
                     /locus_tag="VGE92_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007231633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY1313929.1"
                     /translation="MTDKSAASSAADPGVVSFSRMQDGTREDYALLDRAERDYVRGLP
                     DRLLAALARLDHSLLGYPVSRLGHSLQAATRALRAGADDELIVAALIHDIGDDLAPYN
                     HSQIAAAIIRPYVRPEVTWIVEQHGLFQTYYFAHHSGGDRHARDKFRDHPWYQACKDF
                     CADWDQCSFDPRYPTEPLSTFEPRLREIFSRPAHDARYLGAT"
     gene            complement(3190..4311)
                     /locus_tag="VGE92_08630"
     CDS             complement(3190..4311)
                     /locus_tag="VGE92_08630"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY1313930.1"
                     /translation="MNCSRLHGAKALPALLLSIVCAAGLAGAAPVAEEKRLFIYNWTD
                     FVGHNTIAEFEKQTGIKVTYDVYDSEETMDARLLAGSSGYDVVIASTEYFGREIKAGV
                     YIPLDRSKIPNWKNLDPRILAIQAEHDPGNLHAVPYMHAINGFAYNVDMIKARMPNAP
                     VDSLDMLFKPEIVSKFADCGVTLLDSAADTLLLAMKYLGRDPNSTKREDYQAAEQLLL
                     KVRPYIRSFDSAEYLNGLANQEICIAMSWSSDYALSRARARAVGVNVNLAFTVPKEGA
                     NQNFTSLLIPEGAPHPQAAYRFINFILRPDVIAEITNEIYYGNDNIAARALINPKILN
                     DVTLYPTPEIEARLFRSTEASAATERIRTRAWTRVKTAK"
     gene            4383..5582
                     /locus_tag="VGE92_08635"
     CDS             4383..5582
                     /locus_tag="VGE92_08635"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEY1313931.1"
                     /translation="MKHSRWSRLVFLIMLSMPGASTTSVALAAAAERVRARDLGIPFD
                     GTPGPLNAITDVAGVTVGHTTLISGSGKLQVGHGPVRTGVTAILPRGKDSLSNPVFAG
                     WFAQNGNGEMTGTTWVEESGFLEGPLMITNTHSVGVVRDAVIQWRVAHAGADASGFYW
                     SLPVVAETWDGWLNDINGFHVKPEHAIRAIDVARSGAVEEGNVGGGTGMICNGYKGGI
                     GTSSRHLDSKQGGYTIGVLVQCNYGTRDNLLIAGVPVGREIPAPEPYAFMPSDIAERG
                     SIIVVVATDAPLLAHQLKRIARRVTLGVGRNGSISGNDSGDIFIAFSTANSGAGAASK
                     VVDLKMLPNDSLDPVFRATVEATEEAIVNAMIAAQDMTGADNHHVIALPHAALRVILK
                     KYGRLAS"
     gene            5605..>6032
                     /locus_tag="VGE92_08640"
     CDS             5605..>6032
                     /locus_tag="VGE92_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313932.1"
                     /translation="MAARFGAALAASLLLALGTHPTAACAAPTAPPAASPAQAEAIYA
                     DWSDAVSAVATIESGLTPKVAGLGRSAWAARLHELTPRLDAALAELDHAALTPQDARV
                     LLALHKGRSENVPEAPVPDPAHPLACGDAARPGSDADTLQ"
BASE COUNT         1063 a         1875 c         1848 g         1246 t
ORIGIN      
        1 ccgccatctg ccaggcgccg cggaataaag cttcgcgctc gaccgcgaat agtcgggcat
       61 cggcgtacac ccaccccggc agacggttat cgagctgcac gctgccgacg ggcggcgctt
      121 gcgcgaattg gccggggaaa atcgagcgca ggtacgccat ggcgcggcgt ttggcggcgg
      181 cacgatcgat ttccgcctcg ctgcggaagg caaagtcctg ccagagcatt tccaggacgc
      241 caatgagacc gagcgcgatt ccatccggat cgagctccgg ccgcgacgtg gcctcgatca
      301 gccgttcgat cagctcacgc accagggcgg caaagctctc gtccttttga ccgcaaatat
      361 cgtagtattc ctggcgtgaa ctcgcctcgc cccaaaacgc ataccaaacc gagactttgc
      421 gaggactggc gatgtcaggg tcaagataca aatcgaccat caattccagc gcctcgaccg
      481 gcctggattt gagccgcgcc acgggcacca ataactgctc ctcgaactcg gccgatagga
      541 attgcaatga tgcgaccagc atggccgcct tcgaggcgaa gtagaaccga acgatgccgg
      601 gcgacatttt cgcaaacgcc gtcaccttct ccaccgtcgt gcgcgacgga ccgtacagat
      661 gcaacgcgga gatgcaggcg tcgatcaagc gctgtcgctg ccggctgtgg gcatcgcgcg
      721 cgcggcgaat gcgggccggc ttagcggttt tcttggaaga cttgcgcatg cgtcggctta
      781 aggtgttatg acaactgcga cccacgaccc gatcatgcac gcgccgttgc gtctgccgcc
      841 gcgccggccc gggattgagt ggctgcagcg ccaaggcaag tggctcatca ttttgccgcc
      901 cctgctgttt cttttggtgt tttttctcat tccgtttggc ttcgcattgg tcatcagctt
      961 tgccgacacg gcggtccggg tgccgccgtt cagcgatatt ctatccgtga gtcgcgactg
     1021 gcacatcaac ctcacgctga atctcggcaa cttcaagtac ttgttcagcg acgaagtgta
     1081 tggcctttcg tacatttatt cgctcaagac cgcgttcttt tcgacgctga tttgcctcgc
     1141 gttgggatat cccatggcct atgccatcgc tcgcacctcg aagagcacgc aaagcatcct
     1201 gctgctggtc atcattctgc cgttctggac ctcgttcctg ctgcgcgtct atgcgctcga
     1261 ggggatcatt cgcgaaaccg gcctcttgaa taccgccctg ctgtggctcg gcatcattca
     1321 tcagccgctg cagatcatgc gcacgaccct tgcggtgtac ctcggcatca tttattccta
     1381 tctgcccttc atggtgctgc cgctgtttgc gactctcgag aagctggatc actccttgtt
     1441 ggaggccgcc ggtgatttgg gcagcccgcc gtggcgggct tttctggaag tcaccttgcc
     1501 cttgtcgatg cccggcatca tcgccggctc gatgttggtg ttcattccgg cgatcggcga
     1561 attcgtgatt ccgacgctgc tcggcggtcc ggataatctg atgatcgggc gggtgctgtg
     1621 ggacgagttc ttcggcaatc gcgattggcc ggtggcctcc gcggtggcca tcgcttttct
     1681 ggtgctgctg gtcgggccga ttgcgctcta ccagcactat aattccaagc agctcgaggc
     1741 ttgaggtgga gaaccgcatc tcgcccttct tggtctcgat tctgtgcatc ggcatcgcga
     1801 tcctctacat tccgatcctg gtcctgatcg gctattcgtt caatgcatcg tcgctggtga
     1861 gcgtctgggg cggcttctcc accagctggt acggcgaact gctgcacaac gaccagattc
     1921 tcgatgcggc attcttaagc ttgaaaatcg gcgtcgtggt gtcgacggga gcggtgattt
     1981 tgggcaccct cgcggcgatt tccctggtgc gcttcaagtt ctttcgcggc cggctgctgc
     2041 tcaccgggat ggtcaacgcg ccgctggtga tgccggaaat catcaccggt atcacccagc
     2101 tgctgctgtt cgtctcgatg ctgcacctac tcggttggcc gcatcgcggc ttcacgacca
     2161 tcgtgatagc gcatatcgct ttctgcagcg cctacgtgac cgtcacggtg caatcacggt
     2221 tggcgagcgc cgaccgctcc ctggaagagg ctgccatgga cttgggatcc gggcccgtgc
     2281 gcgccttcct cgatacgaca ctgccgagca tcgcccccgc tttggcctcg agctggctgc
     2341 tcagcttcac tctgtcgctc gatgatttgg tgatctcgag cttcgtttcg ggtcccggcg
     2401 cgagcacgct gccaatggtg attttctcga aggtgaagct cggtgtcagc ccggacgtca
     2461 acgcgctcgc gagcctcatg atctgtctgg tgggcgcctg cgtgctattc gcaggctatc
     2521 tcatgcgtcg cgccgaacgg cagcgggtgt tggatgcgca gatggccgaa cgctgaagcg
     2581 cgcgtcaagt cgcacccagg tagcgcgcat cgtgcgccgg acggctgaaa atctcgcgca
     2641 ggcgcggctc gaaagtgctc aagggttcgg ttggatagcg cggatcgaac gagcactggt
     2701 cccaatcggc gcagaaatcc ttgcacgcct gataccaggg gtgatcgcga aatttgtcgc
     2761 gcgcatgccg atcgccgccg ctgtgatgcg cgaagtagta cgtttggaac aggccatgtt
     2821 gttcgactat ccaagtaacc tcaggccgca catacggccg gatgatggcc gcggcaatct
     2881 gcgagtgatt gtacggcgcc agatcatcgc cgatatcgtg aatcagtgcc gcgacgatga
     2941 gctcgtcatc cgcgccggca cgcagcgccc gggtcgcggc ttgcagcgag tgtccaagac
     3001 ggctcaccgg gtaacccagc aacgaatgat cgagcctcgc cagcgcggcg agtagacgat
     3061 ccggcaagcc gcgcacgtag tcgcgctcgg cacgatccag caacgcatag tcttcacggg
     3121 tgccgtcctg catccgagaa aaactcacca cgccggggtc ggctgcgctc gatgcggcgg
     3181 atttatcggt catttcgcgg tctttacccg tgtccaggcc cgtgtgcgaa tgcgctcggt
     3241 cgccgcggat gcctcagtgg atctgaacag acgcgcctcg atctcgggag tcggatacag
     3301 cgtgacatca ttcagaatct tgggattgat cagggcgcgc gcggcgatgt tgtcgttgcc
     3361 ataatagatc tcgttggtga tctcggcaat cacatccggc cgcagaatga aattgatgaa
     3421 tcggtacgcg gcctgcgggt gcggcgcgcc ctcgggtatc agcaacgacg taaagttctg
     3481 gttagccccc tccttcggca ccgtgaaggc caggttcaca ttcacgccga ccgcccgcgc
     3541 acgcgccctc gacagcgcgt agtcgctcga ccaggacatc gcgatgcaga tctcctggtt
     3601 ggccaatccg ttcaagtact cggcggaatc gaaagagcga atgtacgggc gcactttgag
     3661 cagcagctgc tcggcggctt ggtaatcctc gcgcttggtc gaattcgggt cgcggccgag
     3721 gtatttcatg gcgagcaaca gcgtatccgc cgccgagtcg agcagcgtga cgccgcagtc
     3781 ggcgaatttc gagacgattt caggtttgaa cagcatgtcg aggctgtcga ccggcgcgtt
     3841 cggcatgcgc gccttgatca tgtcgacatt gtaggcaaag ccattgatgg catgcatgta
     3901 gggtaccgcg tgcaggttgc ccggatcgtg ctcggcttgg atcgccagta tccgcggatc
     3961 gagattcttc cagttgggta tcttggatct atccagcgga atgtagacgc cggccttgat
     4021 ttcgcgtccg aagtattccg tcgaggcaat caccacatcg tagccggagc ttcccgccaa
     4081 cagccgggcg tccatggttt cctcggaatc gtagacgtcg taagtcacct tgatgccggt
     4141 ctgtttttcg aattcggcaa tcgtattgtg gccgacgaaa tcggtccagt tgtagatgaa
     4201 cagccgtttt tcctcggcaa ccggcgcagc gcctgccaag cctgcggcgc agacgatcga
     4261 cagcaacaag gccggcaatg cttttgcgcc gtgaagtcga ctgcagttca tcgagttagt
     4321 ttaccatcgc agcagttctg caggccgtcg acggcgtgca gccatttttt catggagcgc
     4381 acatgaagca cagccgttgg tcccgcctgg tatttctgat catgctgtcg atgcccggcg
     4441 cgtcgacgac cagcgtggct ctggccgcag cagcggagcg tgtgcgcgca cgcgatctcg
     4501 ggattccgtt cgacggcact cccggcccct tgaacgccat taccgatgtg gccggagtca
     4561 ccgtgggcca caccacgctg atttccggat ccggcaaact gcaggtggga catgggccgg
     4621 tgcgcaccgg agtcaccgcc atcctgccgc gcggcaagga ctccttgtcg aacccggtgt
     4681 tcgccggctg gttcgcgcaa aacggcaacg gcgagatgac gggcacgacc tgggtcgagg
     4741 aatccggctt tctcgaaggt ccgctgatga taaccaatac gcacagcgtc ggcgtggtgc
     4801 gcgacgcggt gatccagtgg cgcgtcgccc acgccggcgc ggatgcaagc ggattctatt
     4861 ggtctttgcc ggtggtcgcg gagacttggg acggatggct gaacgacatc aacggctttc
     4921 acgtcaagcc ggaacacgcc atccgtgcca tcgacgttgc gcgctccggt gccgtcgaag
     4981 agggcaatgt gggcggcggc acgggcatga tttgcaacgg ctataagggc ggcatcggca
     5041 cctcctcgcg gcatctcgat tccaagcaag gcggctacac gatcggggtg ctggtgcaat
     5101 gcaattacgg aactcgggac aatttgctca ttgccggcgt accggtcggg cgcgaaatac
     5161 ccgctccgga accctatgcg ttcatgccct cggacatcgc cgagcgcggc tccatcatcg
     5221 tcgtagtcgc gaccgatgca ccgctgctcg cgcaccaatt gaaacggatc gcgcgccgcg
     5281 tgactctggg cgtgggccgc aacggcagca tctcgggcaa cgactcgggc gacatcttca
     5341 tcgcgttctc caccgccaat tcgggggcag gagcggcgag caaggtggtg gatctgaaaa
     5401 tgctgccgaa cgattcgttg gatccggtat ttcgtgcgac ggtggaggcc accgaagagg
     5461 ccattgtcaa tgccatgatc gccgcccagg acatgaccgg cgccgacaat catcatgtca
     5521 ttgctctgcc acacgcggcg ctgcgcgtga tactcaagaa gtatggccgc ttggcttctt
     5581 gagattttgc ggacccgtgg gcgattggcc gcgcgctttg gcgccgctct tgccgcctcc
     5641 ctgctcctgg ctctcggcac gcatcccacc gcagcatgcg ccgcgccaac agcgcccccg
     5701 gcagcatcgc cggcgcaagc cgaggccatc tacgccgatt ggtcggatgc ggtcagcgcc
     5761 gtggcgacca ttgagtctgg actcacgccc aaagtcgccg gcctcggacg cagcgcctgg
     5821 gcggcgcgct tgcacgaatt gaccccgcgg ctcgacgccg cgctcgccga actcgaccat
     5881 gccgccttga cgccgcagga tgcgcgcgtc ttgctggcgc tacacaaggg acggagcgag
     5941 aacgtgcccg aagcgccggt tcccgacccc gcgcatccgc ttgcctgcgg cgacgctgca
     6001 cggcccggca gcgatgccga cacgctgcag at
//
LOCUS       DASWZT010000314         3308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_21860, whole genome shotgun sequence.
ACCESSION   DASWZT010000314 DASWZT010000000
VERSION     DASWZT010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21860"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..707
                     /locus_tag="VGE92_08645"
     CDS             147..707
                     /locus_tag="VGE92_08645"
                     /inference="COORDINATES: protein motif:HMM:NF015642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HEY1313933.1"
                     /translation="MSDIRFKDFEDVLAMAGSMADAAEAHGSLCGALCSMAPYKMQDW
                     INEILPDGAALSEESAAMIERVFTATATSFGEQGMEFEPLLPDDEQPLNGRANALALW
                     CTGFLYGLGTGHISDLEALNGDVGEIVRDFTEISRATGDDADADESNEQAYAELVEFI
                     RVAAQVVFEELLPLRRQVYPAQQRLH"
     gene            707..2011
                     /locus_tag="VGE92_08650"
     CDS             707..2011
                     /locus_tag="VGE92_08650"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEY1313934.1"
                     /translation="MDHKEFARRRRQFMHMLGRDAIAILPAAPVRHRNGDIEYAYRQD
                     SHFYYLSGFPEPDAVAVLVPGRGQAEYMLFVREHDPLRESWDGARAGTEGAVERYGAD
                     DAFPIADIDEILPGLMETRSQIYYSMGANPDFDSHVLGWVNGLRVQTRQAAGSPHEFV
                     ALRHVLDDMRLYKSRAEQTSLKRAAKIAVGAHRRAMRFARPGRMEYEVMAEVLHEFRS
                     HNADVSYHPIVGGGANACVMHYRDNDQPLRDGDLLLLDAGCEHDYYASDITRTFPVNG
                     RFTPPQRALYEVVLEAQLAAIDKVRCGNHWNHPHEAAVRVITQGLVRLGLLKGKVASL
                     IKDQAYQQFFSHRTGHWLGLDVHDVGDYKVGGEWRVLEPGMALTVEPGIYVRPSPKVA
                     KEFWNIGVRIEDEVLVTAGAADVLSGALEKTPDAIEALVQAA"
     gene            2008..3246
                     /gene="ubiH"
                     /locus_tag="VGE92_08655"
                     /gene_synonym="visB"
     CDS             2008..3246
                     /gene="ubiH"
                     /locus_tag="VGE92_08655"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF004356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HEY1313935.1"
                     /translation="MTPPPPVDVAIVGGGMVGASLAVALAPLGLKVALIEALAHDSAA
                     QPSFDERTTALSNGSCRILESLGVWSGVMAQATPIRKIHVSDQGHFGFARIDAAEQDL
                     AAMGYVVPNRALGAALWSLLKADGNVQLYCPAQVTRVAAGEEGVALEIARGDVSSAID
                     ARLVVAADGVQSAVRSAFGVDAFARDYQQTAVITTVLPQRFHEHVAYERFTASGPLAL
                     LPLAGGRCTLVLTLDNETAKAAMAWSDDEFIAEVQRRFGFRLGRFLKVGRRVPYALAL
                     NYAERTSARRCVIIGNAAQGLHPVAGMGFNLGLRDVASLAELIAERRHEPHFDAGSPG
                     LLADYDAWRRADRRGVIAFTDGLVRMFASPFGPVKRLRNLGLLAFDLLPPAKAALSRL
                     STGAGGGARVPKLARGVALR"
BASE COUNT          555 a         1028 c         1115 g          610 t
ORIGIN      
        1 tcgagccgct tctccagccg cttcaattcg agttcgaccg ctgacttcgg gttggcttca
       61 ttcataggct gccaatatag agcgccgcat agcgcaatgc aaagggcggt gcaggccctt
      121 actgcgctat tatcggtgta atcatgatgt ccgatattcg attcaaggac ttcgaggacg
      181 tgctggcgat ggccggcagc atggcggatg ccgccgaagc acacggctcg ttgtgcggcg
      241 cgttgtgctc gatggcaccc tacaaaatgc aagactggat caacgagatc ctccccgacg
      301 gcgccgcgct ctccgaggaa tcggcggcga tgatcgaaag agtgttcacc gcaaccgcga
      361 cttcgttcgg cgagcagggc atggagttcg agccgttgct gccggatgac gagcaaccgc
      421 tgaatggccg tgccaatgca ctcgcgctgt ggtgtaccgg gtttctgtat ggcttgggca
      481 ctggacatat ttccgatctc gaggcgctga acggcgacgt cggcgagatc gtgcgcgatt
      541 tcacggaaat ctcgcgtgca accggcgacg acgcggatgc ggatgaatca aatgagcagg
      601 cctatgcgga attggtcgag ttcattcgcg tcgcggccca ggtggtgttc gaagaactcc
      661 tgccgctgcg ccggcaagtc tatccggcgc agcagcggct ccattgatgg accacaagga
      721 gttcgcgcgt cgccggcgcc aattcatgca catgctcggc cgggatgcga tcgcgatcct
      781 gcctgcggcc ccggtacgcc atcgcaacgg cgacattgaa tacgcctatc ggcaggacag
      841 ccacttctac tatctgagcg gatttccgga accggatgcg gtcgcggtgc tggtgcccgg
      901 ccgtggccag gccgaataca tgctgttcgt gcgcgagcac gacccgttgc gcgaatcgtg
      961 ggacggggcg cgcgccggaa ccgaaggtgc ggtcgagcgc tatggggcgg atgacgcgtt
     1021 tccgatcgcc gacatcgacg aaatattgcc gggcctgatg gagacccgct cgcagatcta
     1081 ttattcgatg ggcgccaacc cggacttcga ctcccatgtg ttgggttggg tcaacggcct
     1141 gcgggtgcag acgcgccagg ctgccggctc gccccacgaa tttgttgcac tgaggcatgt
     1201 gctcgacgac atgcggctgt acaagagccg cgccgagcag acgagcctca agcgcgccgc
     1261 caagatcgcc gtgggcgcgc accgccgtgc gatgcgcttc gcgcgtcccg ggcgaatgga
     1321 gtacgaggtg atggcagagg tgttgcacga attccgctcg cacaatgcgg atgtgtccta
     1381 ccaccccatc gtcggcggcg gcgccaatgc ctgcgtcatg cattaccgcg acaacgatca
     1441 gccgctgcgc gacggcgatc tgttgttgct cgatgcgggc tgcgagcatg attactatgc
     1501 ctcggatatc acgcgcacct ttccggtcaa tggccgcttt acgccgccgc aacgggcgct
     1561 ctacgaggtc gtattggaag cgcagctcgc cgcgatcgac aaggttcgtt gcggcaatca
     1621 ctggaatcat ccgcacgaag ccgcggtgcg ggtgattacc caggggctgg tcaggctggg
     1681 gctgctcaag ggcaaggtcg cctcgctcat caaagaccag gcgtaccaac aattcttcag
     1741 ccatcgcacc ggccactggc tcggcctcga cgtccacgat gtcggcgact acaaagtcgg
     1801 cggcgagtgg cgcgtgctgg aacccgggat ggcactcacc gtggaaccgg gaatctacgt
     1861 gcgcccttcg cccaaggtag cgaaggaatt ctggaatatc ggcgtgcgga tcgaggatga
     1921 ggtgctggtc accgccggcg cggcggacgt gctgagcggc gcgctcgaga aaaccccgga
     1981 cgccatcgaa gccttggtgc aggccgcatg acgccgccac ccccggtcga tgtggccatc
     2041 gtcggcggcg gcatggtcgg cgcgagtctc gccgttgcct tggccccgct cgggctgaag
     2101 gttgccctga tcgaagcgct agcgcacgat tctgcggcgc agccgagctt cgatgaacgc
     2161 accacggcct tgtcgaacgg cagttgccgc atcctcgagt cgctcggtgt gtggtccggg
     2221 gtgatggcgc aggcaacgcc gatccgcaaa atccacgtct cggaccaggg ccatttcggc
     2281 ttcgcgcgca tcgatgcagc cgaacaggat ctcgccgcga tgggctacgt cgtgccgaat
     2341 cgtgcgctcg gtgcggcctt gtggtcgctg ttgaaggccg atggcaacgt acagctgtac
     2401 tgcccggcgc aggtcacgcg cgtcgccgcc ggcgaggagg gggtcgcgct ggaaattgca
     2461 cgaggcgacg tcagcagcgc gatcgacgcc cggctggtgg tagccgccga cggcgtgcag
     2521 tcggccgtgc gcagtgcttt cggggtcgat gccttcgccc gcgactacca gcagaccgcc
     2581 gtcatcacca cggtgctgcc gcaacgcttc cacgagcatg tggcctatga gaggttcacc
     2641 gccagcggac cgttggcgct gctgccgctc gccggcggac gctgcaccct ggtgttgacg
     2701 ttggacaacg agaccgccaa ggccgcgatg gcgtggtctg acgatgagtt tatcgccgaa
     2761 gtgcagcgcc gcttcggatt caggctcggg cggtttctga aagtcggacg ccgcgtgcct
     2821 tacgcgctcg cgctcaacta tgccgagcgc accagcgccc ggcgctgcgt gatcatcggt
     2881 aacgccgctc aggggctgca ccccgtggcg gggatgggtt tcaacctcgg acttcgcgat
     2941 gtcgcgagtc tcgccgagct catcgccgag cgccgccacg agccgcactt cgatgcgggt
     3001 tcccccggtc tgttggccga ttacgatgcc tggcgccggg ccgatcgacg cggcgtgatc
     3061 gcgttcacgg atggcttggt gcggatgttc gccagcccat tcggaccggt gaagcgtctg
     3121 cgcaatctcg gcttgctggc cttcgacttg ttgccgccgg ccaaggcggc cctgtcgcgg
     3181 ttgagtacgg gtgcgggcgg cggtgcgcga gttccgaagc tggcgcgcgg cgtcgcgttg
     3241 cgatgagtac gccgcatgaa ttcgacgtcg tcatcgtcgg tgccggcgtc gtcggcagcg
     3301 tcatggcg
//
LOCUS       DASWZT010000315         6598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_12218, whole genome shotgun sequence.
ACCESSION   DASWZT010000315 DASWZT010000000
VERSION     DASWZT010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6598
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12218"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1378
                     /locus_tag="VGE92_08660"
     CDS             <1..1378
                     /locus_tag="VGE92_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313936.1"
                     /translation="PGTEQPRLDHGIFRLAVHDIPAFVSEEYMPPAEELQMRVDFVYL
                     NDSQIDKDSAAFWQRHAKTMYRTIDRFMDMRRAMQDAVAKTVSPEDSAEDKLRKLYAR
                     CQQIRNLSAERARSAQEVEREGSKDNHDVADVWKNGYGNRHQINWLFVALARAAGFEA
                     DPVLVATRTRYFFNAKMENPSQLNSAMAVVKLNGQQKFLDPGTPFAPFGALPWGMTAV
                     AGLRLDANGGSWIETPLPSAADSSIERRASLRLAPDGSLEGKVSLTFNGLQALSRRLE
                     LRNEDDTARTRFLEGAVKGDIGSGCQATLSNTPDWTGANSTLVAEFDVQVPGWATVAG
                     RRTLVPSGLFAGGSRHAFQHATRIHPVYFHYPQRVADDISIELPPGFEVESMPKPFRA
                     NITVANYEFSAQNSGTVLHLTRVITVSSLIVPAANYGLLQDFFESVRTADTEQTVLLG
                     PAATAALP"
     gene            1375..3351
                     /locus_tag="VGE92_08665"
     CDS             1375..3351
                     /locus_tag="VGE92_08665"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY1313937.1"
                     /translation="MKGGRAIRHLALACLMAPYAVHAASAPAWMHALVTAPLPAHTDK
                     TNAALVYADISVTVEPSGKIRRVERRAYQILRPDGAVRGKLGFVIDGKTHVTDLHAWS
                     IPAAGKDYEVNKHDAVETAVDGLNGILVTDIHLLLLQIPAALPGNLVGYEVQEENTPY
                     VLEEAWNFQDVIPVRETHYHLQLPNGWSYRATWLNHEELAPTVSGPNQWQWTLTDVAA
                     LPTEPRMPPLAGVAGRMFVALIPPPGHGQSVQSWSDLGIWFTGLAAGRRTASPELKQK
                     VAELTASSSALPAKMQALAVFAQDDIRYVGIELGIGGYQPHSAAEVFTHRFGDCKDKV
                     TLLSGMLAELGIPSYYVLINTQRGAVTPRTPPNTYFNHAVLAVALPADLDDPRLEAVL
                     EHPKLGKLLFFDPTDALTPFGSLSGALQGNYGLLVTPDGGELLELPVLAPASNGIART
                     ATLTLDDAGTLRGEVHEARVGQQAAAQRYLLRSAKVDTDQIKPLEAVLAASLPSFQIT
                     KASISNLRATDKPFQWQYSIEADHYARAQGELLLVRPRVIGSKAQGFLDAKESRHYPI
                     EFEEPERDTDVFEIALPAGYSVDQLPAAVDVVGDFASYHSKTELVGRTLKYTRSFEIA
                     KLSAPASSAEDLKALFKTIENDERSAAVLKHAAP"
     gene            3443..>6598
                     /locus_tag="VGE92_08670"
     CDS             3443..>6598
                     /locus_tag="VGE92_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY1313938.1"
                     /translation="MESSRGVAILAALLALSALAADALPGAASSDADVIIILRDQVEA
                     VPATRDLRAARTAALALSRAPLLAELERAQARKVHAFNMINAVAATVTSAEATQLAAH
                     PLVQAVVPDAVIRLLRPKRNAARAAAGATTGAGPGTAGSGALCNTLEPEALQLTHTAF
                     LDPTIPQAQQVIDGEGHTVTGRGVKVAFLADGLDPTLPGYVRPDGTNVFIDYQDFSGD
                     SPGTPTAGGEAFGDSSSIAAQDYPNGKLLTFDLDSYVLPGSSLPAPCKIRIRGMAPDA
                     SLVGLKIFSNLGYTTTSGFVQAVEYAVLHDDVDIINESFSSFYNPDNDNDPISLANEA
                     AVKAGVTVVGITYDGGSAGTFESSGTDPWMIAVGASTQYRLYAQTQAPLGSLTKPGAG
                     YLDNNISALSSAGFAQTGTRTTDVVAPGDLGWALCSLDQALYYDCGSNNSFDPAAIQE
                     FGGTSESAPLTSGEAALIIQAYRSTHGGASPSPALVKSIIMSTATDLGAPASEQGAGL
                     IDALAAVRLALSIKDGHGGAKGPGGLLTSPTSTGFTDNENAAKSVSFSITNTGSTPQR
                     IAPHLQTLSPAFAGATVSVELDAAKAPQFIDPYGYANAYVKHTFSVPAGAQHLDAAIA
                     FVTPAGSAPLVQLVLLDPLGRLATYSLPQGTGSGYGHVDVVSPTAGLWTAVIFAYPPS
                     VAPALTYSGPVQFTWSAERFVDLGSVTPASVELAGGASATFSAKFNMPSTPGDLAAGI
                     RFERTGGLTPGEIPVTLRTLIPVGPTGGSFSGTLTGGNGRAQIAPTQIFAFDVTSPAQ
                     SMSLTLNIADSGDPLVGILVDPNGMPLSVGGNADLAGDPEYGFQLSRFNPQLGRWRFL
                     LLQNFTTSGNQTSLPFVARIAFNSERISAPALPDAPSSTVSASGAALVVPVHVTNTGV
                     VTQAYFADARLRTPAQTSLTALPLCTVVTLPGTCSEFVLPTEVSGAVFTARSPAPITM
                     DVSVDVGASPAVFVDAPDIYARKIGPTTVAASISEPELPYSAWVETPALIGPYGPAGA
                     LSQPVSTSAEVWMLPFDPAVSS"
BASE COUNT         1173 a         2149 c         2075 g         1201 t
ORIGIN      
        1 gcctggaaca gagcagccga gattggacca cggcattttt cgcttggccg tgcatgacat
       61 tccggccttc gtcagcgaag agtacatgcc gcccgccgaa gagctgcaga tgcgggtcga
      121 cttcgtctac ctcaacgatt cgcaaatcga caaagattcc gccgcctttt ggcagcgcca
      181 tgcgaagacg atgtatcgca ccatcgatcg attcatggac atgcgtcgcg cgatgcagga
      241 tgccgtcgcc aagaccgtct cgcccgagga ctccgcggaa gacaagttgc gcaagctcta
      301 tgcgcgctgt cagcaaatcc gcaatctatc ggccgaacgg gccaggagcg cccaggaagt
      361 cgagcgcgaa ggctcgaagg acaatcacga tgtggccgac gtgtggaaga acggctatgg
      421 caatcggcac cagattaact ggttgttcgt ggcgctcgcg cgcgccgccg gattcgaggc
      481 ggatccggtg ctggtcgcga cgcgaacccg gtattttttc aatgccaaaa tggagaaccc
      541 gtcgcagctc aactccgcga tggcggtggt caagctcaat ggccagcaga aatttctcga
      601 tcccggcacg ccgtttgcgc cgttcggtgc gctgccgtgg ggcatgaccg ccgtggctgg
      661 gctgcggctc gatgcgaacg gcggctcgtg gatcgagacc ccgctgccaa gcgccgctga
      721 ctcgagcatc gaacgacggg ccagcctgcg actggcgccc gatggttcgc tcgagggcaa
      781 agtgagcctc acgttcaatg gattgcaggc cctctcgcgg cgcctggagt tgcgcaacga
      841 ggacgacacc gcccgcacgc gctttctgga aggcgcagtt aaaggcgaca tcggcagcgg
      901 ctgccaggcg actctcagca atacgccgga ctggacgggc gccaattcga cgttggtggc
      961 tgagttcgac gtgcaggttc ccggctgggc gaccgtcgcc gggcgccgga cgctcgtgcc
     1021 ctcgggactg tttgccgggg gcagccggca tgcgttccag cacgcgaccc gcatccatcc
     1081 cgtgtatttc cattacccgc agcgggtcgc agacgacatc tcgatcgagt tgccgccggg
     1141 ctttgaagtc gagagcatgc cgaagccctt tcgagcgaac atcaccgtcg ccaactacga
     1201 attctcggcg cagaactcgg gtacggtatt gcatctgacg cgcgtgatca cggtatcgtc
     1261 gttgatcgtc ccggcggcga attacggact gctgcaagat ttcttcgagt cggtgcgcac
     1321 tgcggatacc gaacagaccg tgctgttggg ccccgcggcc accgcggcgc tgccatgaag
     1381 ggcggccgcg ccattcgaca tctcgccctc gcctgcctga tggcccccta cgccgtgcac
     1441 gcggcgagcg cccccgcgtg gatgcacgcg ctcgtaaccg cgccgctgcc cgcgcacacg
     1501 gacaaaacca atgcggcgct ggtgtatgcc gatatatccg tcaccgtcga gccgagcggc
     1561 aagatccggc gcgtggagcg cagggcatac cagatcttaa ggcccgacgg tgcggtgcgc
     1621 ggcaagctcg ggtttgtcat cgatggcaag acccacgtga ccgatcttca tgcctggagt
     1681 attccggccg ccgggaagga ctacgaggtc aacaagcacg atgccgtcga gacggcggtc
     1741 gatgggctca acggcattct ggtcaccgat attcatctct tgttgctgca gattcccgcg
     1801 gcgttaccgg gcaacctcgt cggttacgag gtgcaggagg aaaacacgcc ctacgtcctc
     1861 gaggaagcgt ggaatttcca ggacgtcatt ccggtgcgcg aaactcacta ccatctgcag
     1921 ctcccgaacg gctggagcta ccgggcgacg tggctcaacc acgaggaact cgcgcccacc
     1981 gtgtccgggc cgaatcaatg gcagtggacg ctgaccgatg tggctgcgct gcctacggaa
     2041 ccgcgcatgc ctccgctcgc cggggtcgcc gggcgaatgt tcgtcgcgtt gattccgccg
     2101 ccgggacatg ggcagagcgt gcaaagctgg agcgatctcg gcatctggtt cacgggcctc
     2161 gcagcgggtc gacgcaccgc ctcgcccgag ttgaaacaga aagtcgccga gttgactgcg
     2221 tcatcgtccg cactgccggc gaagatgcag gcgttggccg tgttcgcgca ggacgatatt
     2281 cgctatgtcg gtatcgagct tgggatcggc ggctaccaac cgcatagcgc ggcggaagtg
     2341 ttcacccatc gattcgggga ttgcaaagac aaggtgacct tgctgagcgg catgctcgcc
     2401 gagctcggca tcccctccta ttacgttctc atcaatacgc aacgcggcgc cgtgacgccg
     2461 cggacgccgc cgaataccta cttcaatcac gccgtgcttg ccgtcgcact gcccgcggat
     2521 ctcgatgatc cgcgcctcga ggcggttcta gagcatccga agctgggcaa gctgctcttt
     2581 ttcgatccga ccgacgcctt gacgccgttc ggcagtttgt cgggcgcgct gcaaggcaac
     2641 tatggcctgc tggtgactcc ggacggcggc gagttgctcg aactccccgt gctggcgccg
     2701 gcgagcaatg gcatcgcgcg cacggcgacc ctgaccttgg atgatgcggg aacgctgcgg
     2761 ggagaagttc acgaagcgcg tgttgggcag caggcggcgg cgcagcgcta tcttttgcgc
     2821 agcgccaagg tcgacaccga ccagatcaag ccgctcgaag ccgtgctcgc ggcgtcgctg
     2881 ccgtcattcc agatcaccaa ggccagcatt tcgaatctgc gcgccaccga caaaccgttc
     2941 caatggcagt attcgatcga ggccgatcat tacgcgcgcg cgcaaggcga gctgttgctg
     3001 gtgcggccgc gggtgatcgg cagcaaggca caaggatttt tggacgcgaa ggagtcgcga
     3061 cactatccga tcgaattcga ggagcccgag cgcgataccg atgttttcga gatcgcgctg
     3121 ccggcgggat acagcgtcga tcagcttccc gccgcggtcg atgtcgtcgg cgattttgcg
     3181 tcctatcaca gcaagacgga actcgtggga cggaccctca agtacactcg atccttcgag
     3241 atcgcgaagt tgagcgcgcc cgcgagcagt gcggaggatc tcaaggcgct gttcaaaacg
     3301 atcgagaacg atgaacgcag tgcggctgtc ctgaaacacg ctgcgccata aaataatcga
     3361 aacgccttct tttccgtgat cgatcacggt ataagcaacg caaagtcgcg atcatccgcg
     3421 attaaaaaaa gtgaggggat gcatggaaag ctccaggggt gttgcgattt tggcggcatt
     3481 gcttgcctta tccgctttgg ccgccgacgc gctgcccggt gccgcctcga gcgatgcgga
     3541 cgtgatcatc atcttgcgcg accaagtgga agccgtcccc gccacgcgcg atttgcgtgc
     3601 cgcgcgcacg gcggcgctcg ccctgtcgcg tgccccgctc ctcgcggagc tcgagcgcgc
     3661 gcaagcacgc aaggttcacg ccttcaacat gatcaatgcg gtggccgcaa ccgtgacaag
     3721 cgccgaggcg acgcagctcg ccgcgcatcc tctggtgcag gccgtggtcc cggatgcggt
     3781 cattcggttg ctgcgtccga aacgaaacgc cgcgcgcgcc gctgcgggtg caaccaccgg
     3841 tgccggtccc gggaccgcgg gctcaggagc cctttgcaac acgctcgagc ccgaggcctt
     3901 gcaattgacg cacacggcgt tcctcgatcc gaccattccg caggctcaac aggtcatcga
     3961 cggtgagggt cacacggtga cgggccgcgg cgtcaaggtg gccttcctcg ccgacgggct
     4021 ggatccgacc ctcccgggct acgtgcgtcc cgatggaacg aatgtgttca tcgactacca
     4081 ggacttttcc ggggattcgc ccggcacacc caccgccggc ggcgaggcgt tcggcgattc
     4141 gagctcgatc gcagcccagg attaccccaa cggcaagctg ctgacgtttg atctcgattc
     4201 ctacgtgttg ccgggctcct ccttgcccgc gccgtgcaag atccggatcc gcggcatggc
     4261 tcccgacgct tcgctggtcg ggctcaagat cttttcgaac ttgggctaca ccaccacgtc
     4321 cggcttcgtg caggccgtcg aatacgcggt actgcacgat gatgtcgaca tcatcaacga
     4381 atccttctcg agcttctata atcccgacaa cgacaacgat cccatctccc tcgccaacga
     4441 ggccgcggtg aaggcgggcg tgaccgtagt tggaatcacc tatgacggcg gttcagcggg
     4501 caccttcgaa tcctcaggga ccgatccttg gatgattgcg gtgggggcga gcacccagta
     4561 tcgactgtat gcgcagaccc aagccccgtt gggctcgctc accaaaccgg gcgcgggcta
     4621 cctcgacaac aacatctcgg cgttgagctc ggccggtttc gcgcaaaccg gcacgcgcac
     4681 cacggatgtc gtcgcgcccg gggacttggg ctgggcgctg tgctcgctcg atcaagcgct
     4741 gtactatgat tgcggcagca ataattcctt cgatccggcc gcgatccagg agtttggggg
     4801 taccagcgag tcggcgcctc tcacctcggg ggaggcggcg ctcatcatcc aggcctaccg
     4861 cagcacgcac ggcggtgcga gtccctcgcc ggcgctcgtc aagagcatca tcatgagtac
     4921 cgccaccgat ctcggggccc ccgcctccga gcagggtgca ggtctcatcg acgcgctcgc
     4981 tgcggtgcgc ctcgcgctca gcatcaagga tggccacggc ggagcgaagg gacccggcgg
     5041 cttgctgacc agtcccactt ccaccgggtt caccgataac gagaacgcgg cgaagagcgt
     5101 ttcgttctcg atcaccaaca ccggctcgac cccccagcgc attgcgccgc atctgcagac
     5161 cttgagcccg gcgtttgccg gggcgacggt ctcggtggag ctcgatgccg cgaaggcgcc
     5221 gcaattcatc gacccatacg ggtacgcgaa tgcctacgtc aaacacacct tcagcgtgcc
     5281 cgccggcgcg cagcacttgg atgccgcgat cgccttcgtc acgcccgccg gtagcgcacc
     5341 actcgtgcag ctggtgctgc tcgatccttt gggacgcctc gccacctatt ccctgccgca
     5401 gggtaccggc agcggctacg gccacgtcga tgtcgtaagt ccgaccgcag gtctttggac
     5461 cgcggtgata tttgcgtatc cgccgagtgt cgcgccggcg ctcacctatt cggggcccgt
     5521 tcaattcacc tggtcggcgg agcgctttgt cgatctcgga tcggtgacgc cggcaagcgt
     5581 tgagcttgcg ggcggtgcga gcgcgacctt tagcgccaag ttcaacatgc cgagcacgcc
     5641 cggtgatctc gctgcgggaa ttcgcttcga gcgcacgggg ggtttgaccc ccggagaaat
     5701 cccggtcacc ttgcggacct tgattccggt cggtcccacc ggcggctcct ttagcgggac
     5761 gctcaccggc ggaaatggcc gcgcccaaat cgcgcccacg caaatcttcg cgttcgacgt
     5821 gacgagcccg gcgcaaagca tgagcctcac cttgaacatc gcggacagcg gcgatccctt
     5881 ggtgggaatc ctggtcgacc cgaatggcat gccattgagc gtgggcggca atgctgatct
     5941 cgcgggggac cctgaatacg gttttcagct ctctcgcttc aacccgcaac ttggccgctg
     6001 gcgcttcctg ctattgcaga atttcacgac gtccggcaac cagacctcgc tgccgttcgt
     6061 cgcgcgcatt gccttcaatt cggagcggat ctcggcgccc gcgctgcccg acgcgccaag
     6121 ctcgaccgtg tcggcgagcg gcgccgcgct cgtggttccg gtgcatgtga ccaataccgg
     6181 cgtcgtgacg caggcctact ttgccgacgc gcggctcaga accccggcgc agacctccct
     6241 gacggcgctg ccgttgtgca cggtggtgac gctccccggg acgtgcagcg aattcgtcct
     6301 gccgaccgag gtgagcgggg cggtatttac cgcccgatcg ccggcgccga ttaccatgga
     6361 tgtgtctgtc gatgtgggcg cgagtccggc ggtgtttgtc gatgcaccgg atatctatgc
     6421 gcggaaaatc ggtcccacga ccgtcgctgc ctcgatcagc gagcccgagc tgccgtacag
     6481 tgcctgggtc gagactcccg cgctcatcgg accctatgga ccggccggcg cattgagcca
     6541 gccggtgtcg acctcggccg aggtatggat gttgccgttc gatccggcgg tctcctcc
//
LOCUS       DASWZT010000316         5031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_50676, whole genome shotgun sequence.
ACCESSION   DASWZT010000316 DASWZT010000000
VERSION     DASWZT010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50676"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VGE92_08675"
     CDS             complement(<1..240)
                     /locus_tag="VGE92_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019692369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEY1313939.1"
                     /translation="MRASTQYSNTQKRGPKPATGENVPVDQVPQDELVLKHADLVKRI
                     AYHLVSRMPPNVEVDDLIQSGMIGLLDAAKHYSPDK"
     gene            complement(237..1124)
                     /locus_tag="VGE92_08680"
     CDS             complement(237..1124)
                     /locus_tag="VGE92_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010449902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HEY1313940.1"
                     /translation="MRDIRLPPERLDELNELNASKPVKVIAVTGGKGGVGKTTISANL
                     AVSFAARGRDVMLLDADLGLANVDVVLGLHTRFHLGHVLRGECELEDAIITGPHGLQI
                     VPAASGITPMANLSATEHAGIIRAFSDLYHRVDILVVDTAAGLHHSVTTFSQAAHHVL
                     VVVCDEPASITDAYALIKVLSREHGVTRFQILANQTRRSGEGPDLFQKIARVCDRFLN
                     VTLEFAGSVPFDDYLRRAVQRQSAVVDAFPACISSIALKNLASKADKWSVPQGARGHL
                     EFFVERMVGARMDPSTVLQ"
     gene            complement(1117..2526)
                     /gene="flhF"
                     /locus_tag="VGE92_08685"
     CDS             complement(1117..2526)
                     /gene="flhF"
                     /locus_tag="VGE92_08685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HEY1313941.1"
                     /translation="MKIKRYAAPTMRAALAQVRSEQGPDAVILSSRRGEEGIEVIAAV
                     DYDEALFVDATRQRVTDRVSAALAAPPPPLPATPIPGAAAPQAAAAAVARAAPMARTA
                     AAFASRQAQLAGETAPPRAPPMARAALATRAAPPRPAAARPQPPAPLQAPAPTRSDPG
                     YVAMQREFQDLRRLLETGLAGMTWSDKRLREPLRAQILEELSALDLAPDVAAALAALA
                     PRRTTLENTSKISLAMLIKHLPVVDDLTVLRGGVIAVVGPTGAGKTTSIAKLAAQWCM
                     QYGSEDLALVSTDGYRIGARDQLMTFARILGAPMHSANNGRELARVLTRLQSKKMVLI
                     DTAGMGPRDARLSEQLAALQIGAARARVLLALPAQGEGHALDEIVKAFAPLSPAACVL
                     TKVDEAASLGAALSTALRHRLKIAYVCDGQRVPEDLHAANQRRVWLVRAALKLKENKP
                     PRSDEAYLIRNFGRVPAHA"
     gene            complement(2552..4633)
                     /gene="flhA"
                     /locus_tag="VGE92_08690"
     CDS             complement(2552..4633)
                     /gene="flhA"
                     /locus_tag="VGE92_08690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEY1313942.1"
                     /translation="MNARATIATFGEFARNGLGVPVLVMVVLAMMVLPLPAFLLDVFF
                     TFNISLSIMILLAVIYVRRALEFATFPTVLLGATLLRLGLNVASTRIVLINGHTGAHA
                     AGHVIAAFGQFVVGGNYAVGMVVFTVLVIINFVVITKGAGRISEVSARFTLDAMPGRQ
                     MAIDADLNAGIITQADAIVRRQEVREEADFYGAMDGASKFVRGDAIAGILVVFINLFG
                     GTIIGAAQHGMSLADAGRTYALLTIGDGLVAQIPALLLSVAVAILVTRVSRPHDMSQQ
                     IMMQVLGQPKALGVTSGILTLLGIIPGMPNLVFLGMAALCAVGAYVFGKRKAAAKAVV
                     AAPAAAAPIPSEQRELSWDDVEPVDLIGLEVGYRLIPLVDRNQGGELMGRIKGVRKKL
                     SEELGFLVQAVHIRDNLELGPNSYRITILGAPVGESEVFPDRELAINPGQVSGTLPGS
                     ATKDPAFGLDALWIEKSRHEQAQGLGYTVVDASSVIATHLSHLLQSHAHELLGHEEVQ
                     QLLNRLGKTAPKLIEDLVPKLLPMSVVVKVLQYLLQERVAIRNLRTICETLAELAPKT
                     QDPVALVAGVRIALGRSIVQNIGGLRQELPVITLDPALEQLLQDSMAAGGDASPGFEP
                     GLADRVQTALADSTRRQEAAGEPAVLLVAPKIRPWISRLMRHSTPTLAVLAYNEIPEN
                     RRIRVIAAVGR"
     gene            complement(4630..>5031)
                     /locus_tag="VGE92_08695"
     CDS             complement(4630..>5031)
                     /locus_tag="VGE92_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEY1313943.1"
                     /translation="QRIRQLQQTLARRRMMHKVPAADVVIVNPTHFAVALKYDPKTMR
                     APRVLAKGLDLVAQNIRRIAEEHRVPVFESPKLARALYRSTDLNKEIPAGLYVAVAQV
                     LSYIFRVRTLNPTIAARLARPNPQVSGDLDP"
BASE COUNT          886 a         1641 c         1614 g          890 t
ORIGIN      
        1 cttgtcgggc gagtagtgct tggcggcgtc gagcaaaccg atcatgccgg attgaatcaa
       61 atcgtcgact tcgacgttcg gcggcatgcg gctgaccagg tgataggcga tgcgtttgac
      121 caaatcggca tgtttcaaca ccaattcgtc ctgcggcacc tgatcgaccg gaacgttctc
      181 gcccgtcgcc ggctttggcc cgcgtttctg tgtattcgag tactgtgtgc ttgctctcat
      241 tgcaataccg ttgaggggtc catccgggcc cctaccatgc gttcgacaaa gaactcaagg
      301 tgacctcgtg ccccttgggg cacagaccac ttatcggcct tggatgccag attcttgagt
      361 gcaatggacg aaatacacgc ggggaacgca tccaccacgg cggattgccg ctgcacggcg
      421 cggcgtaggt aatcgtcaaa cggtacggaa ccggcaaact ccagcgtcac attgagaaac
      481 cggtcacaga ctcgagcaat tttttggaac aggtcgggac cttcgcccga gcggcgtgtc
      541 tgatttgcga ggatctgaaa gcgcgtgacg ccgtgctcgc ggctcaacac cttgatcagc
      601 gcgtaggcat cggtgatcga tgccggctcg tcgcacacca ccaccaacac gtggtgcgcc
      661 gcctggctga aggtcgtgac gctgtggtgc aatcccgccg cggtatccac taccaggata
      721 tcgacccggt gatacaaatc gctgaacgcc cggatgattc cggcgtgctc ggtcgccgac
      781 aggttggcca tgggcgtgat gccggaggcc gccggcacga tttgcaggcc gtgcggtccg
      841 gtgatgatgg catcctccag ctcacattcg cctctgagca catgccctag gtggaatcgc
      901 gtatgcagcc ccaggaccac gtcgacgttg gcgagaccaa ggtcggcatc gagcagcatc
      961 acgtccctgc cgcgcgcggc gaaagagacc gccagattgg cggatatggt ggttttgccc
     1021 acgcctccct tgccgccggt gacggcaatc accttgaccg gtttggacgc attgagctcg
     1081 ttcagttcat cgagacgttc gggggggagt cggatgtcac gcatgggcgg gtactcggcc
     1141 gaagttgcgg atgaggtatg cctcatcgct tcgtggcggt ttgttttctt tcagtttgag
     1201 cgcggcgcgc accagccaca cacgacgctg gttcgctgca tgcagatcct ccggtacgcg
     1261 ctgtccgtcg cacacgtagg caatcttcag ccgatgccgc agcgccgtcg acaacgccgc
     1321 accgagactt gccgcctcgt ccactttggt gagcacgcag gcggcgggcg acagcggcgc
     1381 gaatgctttg acgatttcat cgagtgcatg gccctcgccc tgcgcgggca atgccaatag
     1441 cacgcgggct cgagccgcgc cgatctgcag cgcggcgagc tgttcgctca ggcgggcgtc
     1501 gcgcgggccc atcccggcgg tgtcgatcag caccattttc ttggattgca gccgcgtgag
     1561 gacgcgcgcc aattcccggc cgttgttcgc cgagtgcatc ggcgcgccga gaatgcgtgc
     1621 gaaagtcatc agctggtcgc gagcgccgat gcgatagcca tcggtgctca ccaacgccag
     1681 gtcctcgctg ccgtattgca tgcaccattg cgccgcgagc ttcgcgatgc tcgtggtctt
     1741 gccggctccg gtcggaccga cgacggcaat gacgccgccg cgtaggacgg tcaagtcgtc
     1801 gaccacgggc aggtgcttga tgagcatcgc caaggaaatc ttcgaagtat tttccaacgt
     1861 cgtgcgcctt ggggcgagcg ctgccagcgc cgcggccacg tccggagcca ggtccagcgc
     1921 cgacaattcc tcgagaattt gcgcgcgcaa cggctcgcgc aagcgcttgt cgctccacgt
     1981 catgccggca agcccggtct cgagcaagcg ccgcaagtcc tggaactccc gctgcatggc
     2041 cacataccct gggtcgctgc gcgtcggtgc cggcgcctgc agcggggccg gcggctgcgg
     2101 gcgcgcggct gcgggacgcg gcggcgcggc tcgcgtcgcg agtgccgctc gagccatggg
     2161 cggcgctcga ggaggcgcgg tctcgccggc gagttgcgcc tgtcgggaag cgaacgcagc
     2221 cgcggttcgg gccatcggcg ccgctcgcgc cacggccgcg gcggcggcct gcggcgctgc
     2281 agcgcccggg atgggcgtcg ctggcagggg cggcggcggc gcggcgagcg cagcgctgac
     2341 ccgatccgtc acgcgctggc gcgtcgcatc gacgaacagg gcctcgtcat aatcgacggc
     2401 ggcgatgact tcgatgccct cttcaccgcg gcggctcgac agaatgactg cgtccggccc
     2461 ctgttccgag cgcacctgcg cgagcgcggc gcgcatggtc ggcgctgcgt atcgtttgat
     2521 cttcatgtca acctccgtaa atccagcaaa ctcagcgtcc gaccgccgca atcacccgaa
     2581 tgcggcggtt ttcgggaatc tcgttgtagg caagcaccgc gagcgtgggc gtcgagtggc
     2641 gcatcaggcg cgagatccac gggcgaatct tcggtgccac caacagcacg gccggctcac
     2701 ccgccgcttc ttgacgtcgc gtgctgtcgg cgagcgccgt ctgaactcgg tccgcgagcc
     2761 ccggttcgaa gccgggagag gcatcgccgc ccgccgccat cgagtcttgc agcaactgct
     2821 cgagcgccgg gtccaaggtg atgaccggca actcctggcg caggccgccg atgttctgca
     2881 cgatgctgcg gcccaacgcg atgcgcacgc cggcaaccag cgccaccgga tcctgggttt
     2941 tcggtgcgag ctccgccaac gtctcgcaga tggtgcgcag attgcgaatc gctacccgct
     3001 cctgcagcag gtactgcagc accttcacga cgacgctcat tggcagcagt ttcggcacca
     3061 gatcctcgat cagcttcggc gcagtcttgc cgagtcggtt cagcaactgc tgcacctcct
     3121 cgtggcccaa cagctcgtgc gcgtgcgatt gcaatagatg gctcaagtgg gtcgcgatga
     3181 cgctgctcgc gtcgaccacc gtgtagccga ggccttgtgc ctgctcgtgc cgcgactttt
     3241 cgatccacag cgcgtccaaa ccgaaggccg gatccttggt cgcggatccc ggcaatgttc
     3301 cggagacctg gccggggttg atggcgagtt cgcggtcggg aaacacttcg gactcgccga
     3361 ccggcgcgcc gagaatggtg atgcggtatg agttcgggcc gagttccaaa ttgtcgcgaa
     3421 tgtgcacggc ttgtaccagg aagccgagct cctcggacaa tttcttgcga actcccttga
     3481 tgcgccccat caattcgccg ccctgattcc gatccaccag cggaatcagc cgataaccca
     3541 cctcgagccc gatcaaatcg acgggctcca catcgtccca ggacagttcg cgctgttcgg
     3601 atggaatcgg tgccgcggcg gcgggcgccg ccaccaccgc ctttgccgcc gccttgcgct
     3661 tgccgaacac gtaggcaccc accgcgcaca gtgccgccat gccgagaaat accaggttcg
     3721 gcatgcccgg gatgatgccg agcagcgtca gtatgccgct ggtcacgccg agcgccttgg
     3781 gctgacccag cacctgcatc atgatttgct ggctcatgtc gtgcggccgc gagacgcgcg
     3841 tcaccagaat cgcgaccgcg accgacagca gcagcgccgg aatctgcgcc accaatccgt
     3901 cgccgatggt gagcagcgca taggtgcggc cggcatcggc gagcgacatg ccgtgctggg
     3961 cggcgccgat gatggtgccg ccgaataaat tgatgaacac gaccaggatg ccggcgatgg
     4021 catcgccgcg cacgaacttg ctcgcgccat ccatcgcgcc gtagaagtcg gcctcctcgc
     4081 gaacttcctg acggcgcacg atggcgtcgg cctgggtgat gatgccggcg ttcaaatccg
     4141 catcgatcgc catctgccgg ccgggcatcg cgtccaaggt aaatcgcgcg ctgacttccg
     4201 agatgcggcc agcgcccttg gtgatgacaa cgaagttgat gatcacgagc acggtgaaca
     4261 ccaccatgcc cacggcgtaa ttgccgccga ccacgaactg gccgaatgcc gcgatcacgt
     4321 gccccgccgc atgcgcgccg gtgtggccgt tgatcaacac gatacgcgtc gaagcgacgt
     4381 tgagccccag gcgcagcagc gtcgcgccca acagcacggt cgggaaggtc gcaaattcga
     4441 gcgcccgtct gacgtagatc accgccagca agatcatgat ggacagcgaa atgttgaaag
     4501 tgaagaaaac gtcgagcaag aacgccggca gcggcaacac catcatggcg agaaccacca
     4561 tgaccagaac cggcacgccc aagccgttgc gcgcgaattc gccgaaagtc gctatcgtgg
     4621 cgcgtgcgtt catgggtcga gatcgccgct aacttgcgga ttggggcgcg ccaatcgcgc
     4681 cgcaatggtc gggttcaggg tgcgcactct gaagatgtac gacagcacct gcgccacggc
     4741 cacgtacagg ccggccggga tttccttgtt caaatccgtc gagcgataca gcgcccgcgc
     4801 cagtttcggc gattcaaaca ccggcacgcg atgttcctcg gcaatgcggc ggatattctg
     4861 cgcgacgagg tcgaggccct tggcgagtac gcgcggcgcg cgcatggtct tcgggtcgta
     4921 tttcaaggcc accgcaaagt gcgtcggatt gacgatcacc acgtcggcgg ccggcacctt
     4981 gtgcatcatg cggcgccgtg ccagcgtctg ctgcagctgc cggatgcgct g
//
LOCUS       DASWZT010000317         3311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_74959, whole genome shotgun sequence.
ACCESSION   DASWZT010000317 DASWZT010000000
VERSION     DASWZT010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74959"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1454)
                     /gene="ppnN"
                     /locus_tag="VGE92_08700"
     CDS             complement(69..1454)
                     /gene="ppnN"
                     /locus_tag="VGE92_08700"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF038390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HEY1313944.1"
                     /translation="METTILSSDMVDARVAPKGSLEHLSQQEIAKLLDSGQGGLYPLF
                     RKCALAVLNSGSDIDDARLIFEKYRDFDLRIVRQAWGIKLEIKNAPAAAFVDGVMIRG
                     LKDHLFAVLRDVVFISNEIMESGRFDLTSSASITNAVFHILRNARVLELQMRPNLVVC
                     WGGHSIRRVEYDYTKKVGYELGLRGLDVCTGCGPGAMKGPMKGATIGHSKQRIVDGRY
                     LGVTEPGIIAAEPPNPIVSQLVIMPDIEKRLEAFVRLGHGIVIFPGGAGTAEEILYLL
                     GILLDPANRDLPFPVVFTGPADSAEYFEQIHRFIGATLGSAAQTRYQMIIDDPAGVAR
                     AMVQGMSQVRDFRRQASDSYGFNWLLKIPLEFQQPFEVSHASMRALRLSRDQPAHDRA
                     ANLRRAFSGIVAGNIKEYGINAIERRGPFELAGEPSIMEPLDRLLRSFVAQGRMKFTG
                     TEYTPCYRLVA"
     gene            complement(1465..1995)
                     /locus_tag="VGE92_08705"
     CDS             complement(1465..1995)
                     /locus_tag="VGE92_08705"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY1313945.1"
                     /translation="MSKRIGRHPASLWAVLALAGCLTAPLRPALAEGNIAAADRVVVH
                     KAEHKLYLYNGNRLLGEYKVQLGLSPSGQKEREHDFRTPEGRYYLARRNTRSEFFLAI
                     QVSYPNKEDELRARKNRWAPGGSIMIHGFPNTPKHPAAYYESNDWTDGCIALSNSDMV
                     EVWMRTQDNIPIDIYP"
     gene            2066..2641
                     /locus_tag="VGE92_08710"
     CDS             2066..2641
                     /locus_tag="VGE92_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313946.1"
                     /translation="MRHAVIPLILLVAGLAGCETQSSVKPVETLDERTGVTVGALKEA
                     IELVPTTQNAVLSLRRRATFAYLGPVELDRAGTISYGLWIHIAPGNDHQPGDIHATGA
                     LTLIMDDGSLVLSVIDAPKLGHDPYQGAVSWGQTAYFNLDVDMLRRMAASRKLDLDVL
                     AVGGTVIHFAPTLDTRPVLAQYLQARGITGD"
     gene            complement(2628..>3311)
                     /locus_tag="VGE92_08715"
     CDS             complement(2628..>3311)
                     /locus_tag="VGE92_08715"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEY1313947.1"
                     /translation="LAANGWGENRNVSRGESVRIPLPASRAEGAAAPVILAAALPAPL
                     PEASPPMAEIVVQAPKEPVSQRQTDSNALLPVAAPTGNSDTTDYAVRADGTVIVQAAE
                     TLGHYADWSGVSSQALRALNKLHKNAMVTLGHKVKLDFSKVSAEQFAATRREYHHRLQ
                     DDFFAAHRIAGTDNYTVKRGESLWQIAQQHADLPVWLVAQYNPDVDFNDVRPGTAIAL
                     PRVVAINRQ"
BASE COUNT          619 a         1026 c         1013 g          653 t
ORIGIN      
        1 attgaattcc accgacttct gggatccatc cggcaaatcg aatcgatagc tgatgcgctg
       61 cgaatcgctc atgccacgag ccgatagcaa ggcgtgtact cggtgccggt aaatttcatg
      121 cggccctgcg caacgaaaga gcgcagcagg cgatcgagcg gctccatgat ggacggctcg
      181 cccgcaagtt cgaagggtcc gcggcgttcg atcgcgttga tgccgtattc cttgatgttc
      241 cccgcaacga tacctgaaaa cgcgcgcctc aggttcgcgg cgcggtcgtg cgccggctgg
      301 tcgcgcgata gccgtaaggc ccgcatcgag gcatgtgaga cctcgaatgg ctgttgaaac
      361 tccagcggga tcttcagcag ccagttgaag ccgtacgaat cgctggcctg tcggcgaaag
      421 tccctgactt ggctcatgcc ctgcaccatg gcgcgcgcga cgccggccgg gtcatcgatg
      481 atcatctggt agcgggtttg cgccgccgat cccagggtcg cgccgatgaa gcggtgaatc
      541 tgctcgaaat actcggcgct gtcggccggg ccggtgaata ccacgggaaa cggcaaatcg
      601 cgattcgccg ggtcgagcag aatgcccagc aggtacagga tctcctcggc cgtgcccgcg
      661 ccgcctggga aaatgacgat gccgtggccc agccggacga aggcttccag gcgcttttcg
      721 atgtccggca tgatgaccaa ttgactgacg atcgggttgg gcggctctgc tgcaatgatg
      781 ccgggttcgg tgacgcccaa gtagcgaccg tcgacgatgc gctgtttcga gtgaccgatg
      841 gttgcgccct tcatcgggcc tttcattgcg cccggaccgc agccggtgca cacatccaag
      901 ccgcgcaagc cgagttcgta gccgaccttc ttggtgtagt cgtattcgac tcgacggatc
      961 gagtgcccgc cccagcaaac caccagattc ggccgcatct gcagttcgag gacccgcgcg
     1021 ttgcgcaaga tgtgaaacac ggcattggtg atgctggccg aggaagtcag atcgaagcgc
     1081 ccgctttcca tgatctcgtt ggaaatgaac acgacgtcgc gcagcaccgc gaacagatgg
     1141 tctttcagtc cccgaatcat gacgccgtcg acgaaggcgg ccgccggcgc attcttgatc
     1201 tcgagcttga taccccaagc ctggcgcacg atacgcagat cgaaatcgcg atatttctcg
     1261 aagatcagcc gcgcatcgtc gatgtcgctc ccggaattga gcactgccag ggcgcacttg
     1321 cgaaacagcg gataaagacc cccctgcccg gagtcgagta atttggcgat ttcctgctgc
     1381 gagaggtgct cgagactgcc ttttggcgca acccgcgcat cgaccatgtc agaggaaaga
     1441 atcgtggttt ccacgcggca cctgctacgg ataaatatcg atcggaatat tgtcctgggt
     1501 gcgcatccac acttcgacca tatcggagtt ggacaaggca atgcatccat ccgtccaatc
     1561 gttcgattcg taatacgccg cgggatgctt cggcgtattg gggaatccgt gaatcatgat
     1621 cgagccgccg ggtgcccaac gattcttgcg tgcacgcagc tcgtcttctt tatttgggta
     1681 agatacctga atggccagga agaactcgct gcgcgtgttg cggcgggcca gatagtaacg
     1741 gccctcgggg gtccgaaagt catgttcccg ctccttctgt cccgacgggc ttaagcccag
     1801 ctgtactttg tactcgccca gcaagcggtt gccgttgtaa agatacagct tgtgttccgc
     1861 cttgtgcacg acgacgcggt cggcggcggc gatgtttccc tcggccaagg caggccgcag
     1921 cggcgctgtc aggcagcccg ccaacgccag taccgcccaa agactcgccg ggtggcggcc
     1981 gattcgcttg ctcattgtca aaattatagc gtgtaggcgc tgctccataa tcggttctag
     2041 ctcacgtccc ggtatactgg gaccgatgcg ccatgcagta attccactga tcttgctcgt
     2101 cgcggggctg gccggttgcg aaacccaatc ctccgtgaag cccgttgaaa ccctggacga
     2161 gcgcaccggg gtgaccgtgg gcgcgctcaa ggaggcgata gaactcgtcc cgaccacgca
     2221 aaatgcggtg ctgtcgttgc gcaggcgagc gacctttgcc tacctgggcc cggtcgaatt
     2281 ggatcgcgcc ggtaccatca gctacggcct gtggatacac attgcgccgg gaaacgacca
     2341 tcagcccggc gacattcacg cgaccggcgc gctcacgttg atcatggatg acggttcgct
     2401 ggtgctgtcg gtcatcgatg cgccgaagct cgggcacgat ccgtaccagg gggcggtctc
     2461 ctggggccaa acggcgtact tcaacctcga tgtcgacatg ctgaggcgca tggctgccag
     2521 ccgcaaactc gatttggacg tgcttgcggt gggcggtacg gtcatccatt tcgctccaac
     2581 attggatacc cgccccgtgc tcgcccagta cttgcaggca cggggcatta ctggcgattg
     2641 attgccacca cccgcggcag cgcgatggcg gtcccgggcc gtacgtcgtt gaaatccaca
     2701 tcggggttgt attgggccac cagccaaacc ggcagatcgg cgtgctgctg cgcgatctgc
     2761 cacagcgact cgccccgctt caccgtgtaa ttgtcggttc ccgcgatccg atgggccgca
     2821 aaaaaatcgt cctgcaaccg atgatggtac tcgcgtcgcg tggccgcaaa ttgttcggcg
     2881 ctgactttcg agaaatccaa cttcaccttg tggccgagcg tcaccatcgc gttcttgtgc
     2941 agcttgttca aggctcgcag cgcctgcgaa ctgacaccgg accagtcggc gtaatgtccc
     3001 aaggtctcgg ccgcctgcac gatcaccgtg ccgtcggcgc ggacagcgta atccgtggta
     3061 tctgaattgc cggtcggcgc ggccaccggc agcagcgcat tgctgtcggt ttgccgctgc
     3121 gaaaccggtt ccttgggcgc ctgcacgacg atctccgcca tcggcggcga cgcttccggc
     3181 aaaggcgctg gcagcgctgc ggcaaggatc accggtgcag ccgcaccctc cgctcgagac
     3241 gcgggcagcg ggatgcgcac gctctcgccg cggctgacat tgcggttttc gccccagccg
     3301 ttggcggcga g
//
LOCUS       DASWZT010000318        13555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_74963, whole genome shotgun sequence.
ACCESSION   DASWZT010000318 DASWZT010000000
VERSION     DASWZT010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13555
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74963"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VGE92_08720"
     CDS             complement(<1..802)
                     /locus_tag="VGE92_08720"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEY1313948.1"
                     /translation="MRIACISCGLALGGLLSVAAAPSAAGAPEPDTADPLAREFLQPP
                     NSAAPRVWWHWMNGNITKEGVQLDLAWMKRIGIGGFHNFDASLGTPQVVEQRLAFMTA
                     PWKDAFAYTVTLAQQLGLEMAIAGSPGWSETGGPWVAPKDGMKKVVWSETQVPGGKRF
                     RGTLPRPPGITGPFQSKRAEFLNFGGGEQIPPPEFYADSAVIAYRIPVHGVYPSALQA
                     ALSASAGTPDIAAIGRADSSGVVSLPMAAGGGVNWIQYELKRPQTIYGV"
     gene            complement(804..1505)
                     /locus_tag="VGE92_08725"
     CDS             complement(804..1505)
                     /locus_tag="VGE92_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEY1313949.1"
                     /translation="MSEVDTVSRRSFLSMTGMAALASARPAVLEAAAQTRRPAAPAAG
                     KWPAGFLWGAATAGHQVEGSNVNSDIWMLEHVKPTIFAESSGDACDQYQRYAADIATL
                     AELGFNSYRFSLEWARIGKDGPIAPDSGVEVTQMGEEFYPEALENTVRYAAAETRKPV
                     YVTENVRGYIHWSLLDNFEWLFGYRPKFGLIAVDRKTQTRTIKPSGRHLGDLARSNGS
                     RDADGIHVRHQHVKS"
     gene            complement(1626..2537)
                     /locus_tag="VGE92_08730"
     CDS             complement(1626..2537)
                     /locus_tag="VGE92_08730"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY1313950.1"
                     /translation="MNLNRLDLNLLVALDALLAERSITHAAERLNLSPSATSGALARL
                     RIYFDDELLTQIGRRMVPTPLGESLEGSVRDCLLHVQATVDIRPKFDPLNSKRNFTLM
                     MSDYVSTVLMPAALRQLEREAPGITIELIGNQNEPWEALGRGEIDFLVLPKNFIRDGH
                     PAQVLFEDEYVCVLWNGNTLIGEEISMQQCLQLGHVVARIGTLRPPTIDAWFFERFGH
                     ARRVEVIATSFNSVPHLLIGTNRISIMHRRLARVYAGALPLKILPSPVDMPRLVEMIQ
                     WHKYRDRDPGRVWLCDLLKAAAGGALP"
     gene            complement(2569..3042)
                     /locus_tag="VGE92_08735"
     CDS             complement(2569..3042)
                     /locus_tag="VGE92_08735"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY1313951.1"
                     /translation="MTDSQPRDWRIDGVKVIKSERLDSNTPQTPGMLRAAAVNAARGS
                     QKIWAGTVIVQPNAKTGAHHHGALESVIYVVRGIARMRWGERLEFVAEADAGDFIFVP
                     PYVPHQEINRSSDTPLECVLVRSGNDAVVVNLNIDAAERVEEVYWIDPIHRDAPR"
     gene            complement(3046..4458)
                     /locus_tag="VGE92_08740"
     CDS             complement(3046..4458)
                     /locus_tag="VGE92_08740"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY1313952.1"
                     /translation="MIIIGRLWLSLSFCAAGLGANFAASAAPAAAPDAVRAQAREIFA
                     HIVGIESSIGKGNVPLVAKYLAERFQAGGFPAADIHVLPLGETASLVVRYRGNGHGGR
                     PIDFIAHMDVVTAKRSDWQRDPYHLTEEQGFFFGRGTSDVKQEVALLTAEFLRLKAQG
                     FVPTRDLIIAFSGDEETAQDTARDLVTKHRDLVDAEFALNGDGGGGVLSEGSAKPLIF
                     YVQGAEKSSAQFQLTTHNPGGHSSEPRTDNAIYELADALKAVQRYEFPVKWNEWTVGD
                     FKAASAVTKGPLGEAMAHFAADPGNAAAAAEIAKNPAFIGRIRTTCVATLLSGGHAEN
                     ALPQSASASINCRIFPGTSAAEVQKTLQGLVGPNVQVTQGYDALVSTASPMRADVMNA
                     VAKAVQAADPGAPVVPTQAAYATDGAVFRNAGIPTYGVGGVFIMDSEEFAHGLNERIR
                     VNEFYNGLTYWDVLIRTLAG"
     gene            4510..5595
                     /locus_tag="VGE92_08745"
     CDS             4510..5595
                     /locus_tag="VGE92_08745"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313953.1"
                     /translation="MQSSAQIPVWTIAAPIFAAAVLLTAALGLTAGALAVGLIALGLG
                     GSVFAAVHHAELVAHRIGEPYGTLVLALAVTLIEVALIVSLMAAGGAEAAGLARDTVF
                     AAVMIILNGIIGLCLLVGGIKYREQSFGLDGVSAALVALGTIVILTLVFPNYTVTTPG
                     PFYSPSQLALIAAVTLVVYGAFIFTQTIEHRPLFLDDAKPAQAAPAARPGNWITVIST
                     LLLLACLISVVLLAKKLAPSLEAAVMRVGAPNAVVGVIIAAIVLLPEGLAALQAARGN
                     RLQTSLNLALGSALASIGLTIPAVSFLSLMTNSHLRLGLDSKSSLLLMLSLFVVVVVL
                     RTGRTIILHGIVLLVVFVVYLFTTVVP"
     gene            complement(5603..6280)
                     /locus_tag="VGE92_08750"
     CDS             complement(5603..6280)
                     /locus_tag="VGE92_08750"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY1313954.1"
                     /translation="MARDKTAFLFDLDGTLIDSVYQHVLAWKSSLDQEGIPLSVWRIH
                     RKIGMSGGLFTNILLRETGFDNTPERLERLRVRHAEAYRALSGSVRPLPGARELLDYL
                     TAQGTPWAIATSGRMETAGHNLAALGVDPAKVPVVTRDQVKYAKPDPDLFLTAAERLG
                     VDIRNSLVLGDSIWDMIAAQRARGLGVGLLSGGYGDEELARAGAFRVYEDPADLLKHI
                     DEVAARS"
     gene            6387..7445
                     /locus_tag="VGE92_08755"
     CDS             6387..7445
                     /locus_tag="VGE92_08755"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY1313955.1"
                     /translation="MKHLWMALFVLALNLAAGTVQAEKLRIISWADYVPADLIAAFKK
                     ETGIDVELTLSNNEEIISKLRATGGAGYDLAQPSQDRIVSAQREFGIYKPIDLTKIKL
                     EEFQPQFLDIVRKNTSLDGKVYGLPYLWGTDGLVVNVKRSKIADYSDLCRADLKGKTA
                     IRLRRPTLMAFAFAAGKDPFALYADPKAYSALMDQVGAALIACKANFKFFYDNKDQLL
                     NGIRSGEVVAAMMWDSGGWTLNRENPDIQFIVPRSGALGWLDTFALPARGKNDAAAYA
                     WINFAMRPENAARVVKSVGSFSTAMHTDPLIDPRLKAQFALAFPNNDLKSIHWYPAIP
                     AGLEEIEGKVLDRVKAAN"
     gene            7442..8545
                     /locus_tag="VGE92_08760"
     CDS             7442..8545
                     /locus_tag="VGE92_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1313956.1"
                     /translation="MSTPDLLADAVVKRFADHTAVDSLSFAVARGSFFSILGPSGCGK
                     TTLLRMIAGFIAPDSGDIAIGGASMRGVAPNRRPVNMVFQQLALFPMMSVGENVAFGL
                     ARRGIARAERIKRSNAMLERVGLGNAAHKRVDELSGGQRQRVAIARSLVLEPTLLLLD
                     EPLGALDLKLREHMKIELKQLQAAFGTTFVYITHDQSEALVLSDHVGVMSHGRFEQLG
                     TPQDLYYRPQTPFVAGFVGANNRIVGRASASDGALAEVTSADGWTVRALKSGTIAVGD
                     AVEVFVRPEVASLARNAAELPAGQPRHSGEVQSVLFDGANSAVLLQETQTRREFRIAL
                     PQTGQFSDLKPQEKVAFSFDPSRAVCFAARADA"
     gene            8538..9410
                     /locus_tag="VGE92_08765"
     CDS             8538..9410
                     /locus_tag="VGE92_08765"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1313957.1"
                     /translation="MRDSRWHARLVLGLLLAPAVLWLGALIVLPHVDLAVLSLRERVG
                     PRQYSLSLAQYRTFFAEPLYWHVFVRTAVMSVLATFLTLLLAFPIAWIIAKLAKGRAG
                     SLLFIICLIPFWVSETVRTLGWMILLRESGVLPALMVKLGLTAMPVELLYRDSTILVG
                     LVYTSLLFMVVPLVSSLESLDNSLIEAAYDLGAGGFTILRSIVIPHAAPGITAGCIVV
                     FMLTLGNYLTPTLLGGKNSQWFTEQIYTQFITRFNWEQGAAFGFLLLGLSTALVILGL
                     RISGQRFGDVMRAT"
     gene            9407..10273
                     /locus_tag="VGE92_08770"
     CDS             9407..10273
                     /locus_tag="VGE92_08770"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1313958.1"
                     /translation="MIPSLPRPAWLRAATVAYLIAFLLFLFLPLAVVAVFAFNNAPYP
                     APPWRGFTLDWFLGNAALGRTGLFADSELLGSIWTSCLVALCVTALSAVVGISNSFLI
                     ERGRFPGKQLLSILMLAPLVIPGVILGISILAFASRMADFADDTLGLELDFLRPGLPL
                     VVLGQFSYIATIATLTISARLRRFDRTLEEAALNLGASKAAVFRTILLPYLRPALLGS
                     AAISFLMSFADFNTTLMLVGADTPLTVMMYGRMREGATPVLNAVSLFLMLASAVLALT
                     LLRRSPRPQAAP"
     gene            complement(10297..12195)
                     /locus_tag="VGE92_08775"
     CDS             complement(10297..12195)
                     /locus_tag="VGE92_08775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing diguanylate
                     cyclase"
                     /protein_id="HEY1313959.1"
                     /translation="MRAAESTLASQLEMLEWSDPERAAQMLDGAPPPSSSSVADEIEM
                     LEIRGMIYADSTRDEDVYAVERRLEAIAGTGNDAAVRAGGFVRAYSARQHNQFAAAEA
                     ELKGIDISKIVSDTERYRVLTLRGHVLRILGQDEAALPFLEQALDLAGKMRDDLRTLH
                     AMLSLAHIYTDSGNFDRASVQLNAAMSLATRLGDETSLVETEERKADIADRRGDRAEE
                     RRASLAGLAHAKRSESSKWLALALLNLGDSYLKTRDYAESLKYSKEAMPIIQRTNQRD
                     DETIIQFNEGLAYIGLGSIKMGEKLAERAIGDTLAGSNMLDAKESLREYADALEHAGY
                     LMMAIQVYHRYEAVSEKFMTNTRQRAFLELSAQFDDERKARELELLRRDNALKVSEMR
                     GQRLRQQLILAAALFIAFICGLLVWAFARVSKANERLRFSSEHDPLTGLSNRRYFNER
                     VLAVDGGRPVGGCILLADLDHFKRINDTFGHPAGDAVLAAMSQRLAAALRENDKLVRW
                     GGEEFLAVLGPMSPNQADLTAARLLHAVRCEPVVWNGQVIHCTISIGYACFPMMGSAA
                     DISLEAAISLVDKALYEAKRRGRDRACLIKLVSARDDEELTTISSEFESATSDRRVQL
                     VEILGAAA"
     gene            complement(12438..>13555)
                     /locus_tag="VGE92_08780"
     CDS             complement(12438..>13555)
                     /locus_tag="VGE92_08780"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY1313960.1"
                     /translation="SAVMSAEVQRGRVPGAVALIARRGRIAFFESFGQRDPASAAPMT
                     PDSIFRIYSMTKPITSVAAMMLWEEGKFLLSDPIVKYLPELADLKVAIGHGGMFERVP
                     LERDITIQDLLRHTSGFTYEFRGPGAVQKMYAAAKIARRAQSNAEQVATLGRLPLLHQ
                     PGTRWEYGRSTDVIGRLIEVLSGQTLREFFEQRIFTPLGMVDTAFHVPERYHSRLAEA
                     WPTDPDTGLAVRLLEVRDAPKFESGGGGLVSTAGDYARFLQMLLDLGKFDGQRFLSRK
                     TLELMTADHLGPVTGAPDLLLPGHGFGLGFAVRLHTGIAHVPGTIGQYFWGGLAGTTF
                     WVDPAEQLFAVLLIQAPGQRDYYRTLFRALVYSAVDD"
BASE COUNT         2328 a         4389 c         4190 g         2648 t
ORIGIN      
        1 tcaccccata gatggtctgc ggccgcttga gttcgtactg aatccaattt actcccccgc
       61 ccgcggccat cggcaacgaa accacgccgc tcgaatcggc gcgcccgatg gcggctatat
      121 ccggtgtgcc ggcgctcgcg gacaacgccg cctgcaacgc gctcggataa acgccgtgca
      181 cggggatgcg ataggcaatc acggcgctgt ccgcatagaa ttccggcggc ggtatttgct
      241 cgccgccccc gaaattgaga aattccgcgc gtttgctctg gaacggtccg gtgatgcccg
      301 ggggtctcgg cagcgttccc cggaagcgct tcccgcccgg cacttgcgtc tcgctccaga
      361 caaccttctt catcccatct ttaggcgcga cccacgggcc gcccgtttcg ctccaacccg
      421 gggaaccggc gatggccatc tccaatccga gttgctgcgc caatgtcacc gtgtacgcga
      481 atgcgtcttt ccagggtgca gtcatgaacg ccagacgctg ctcgacgacc tgcggagtac
      541 ccaaggatgc gtcgaaattg tggaagccgc cgataccgat gcgcttcatc cacgcgagat
      601 ccagctgcac gccttctttt gtgatgttgc cgttcatcca atgccaccac acgcggggcg
      661 cggcggaatt cggcggctgc aggaactccc gcgccaacgg atccgctgtg tccggttcgg
      721 gagcgccggc ggcgcttggc gccgcggcga cactcaacaa gccgccgagc gccaagccgc
      781 acgatatgca ggcaattctc acgctacgac ttcacgtgct ggtgtctgac gtggatgcca
      841 tcagcgtccc gagaaccatt gcttcgcgcc agatcgccga gatggcggcc gctgggttta
      901 atggtgcgcg tctgggtctt gcgatcgacg gcgatcaagc cgaatttcgg ccgatagccg
      961 aacagccatt cgaaattatc gagcagcgac cagtggatgt agccgcgcac gttctccgtc
     1021 acatataccg gcttgcgcgt ctcggccgca gcatagcgca ccgtgttctc caatgcctcg
     1081 gggtagaatt cttcgcccat ctgcgtgact tcgacgccgc tgtcgggcgc gataggaccg
     1141 tccttgccga tccgcgccca ctcgagcgag aatcgatagc tgttgaatcc aagctcggcg
     1201 agcgtcgcga tgtctgcggc gtaacgctga tactgatcgc acgcatcgcc ggaactctcg
     1261 gcgaagatcg tcggcttgac gtgctccagc atccagatgt cgctgttgac gttgctgccc
     1321 tcgacttgat gcccggcggt cgcggctccc cacagaaaac ccgccggcca cttgccggcg
     1381 gcgggcgccg cgggcctgcg cgtctgcgct gccgcctcga gcacggccgg acgcgccgag
     1441 gcgagcgccg ccatcccggt catggataga aaactacgcc ggctcaccgt atccacttcg
     1501 ctcatgactc aatcctacca gccgctggaa atccgggggt cgaggctaat cgcgcgcgca
     1561 gcccacgaga aggcgttttc gcggacgcct ggtttcggcg ccgccgaatc ctcggtatct
     1621 tgaggttatg gcaaagcgcc gccggcagcc gccttgagca ggtcgcacaa ccagactctt
     1681 cccggatcgc gatcccgata tttgtgccac tggatcattt ccaccagacg cggcatgtcc
     1741 accggcgagg gtaggatctt gagcggcagg gcgccggcat agacgcgcgc caggcgacga
     1801 tgcatgatcg aaatgcggtt ggtgccgatc aacagatgcg gcacgctgtt gaaactggtc
     1861 gcgatgactt cgacgcgccg cgcatggccg aagcgctcga agaaccacgc atcgatggtc
     1921 ggcggccgca gcgtgccgat gcgcgccacc acgtgcccca gctgcaagca ctgctgcatg
     1981 ctgatctcct cgccgatcaa cgtgttccca ttccacagca cgcacacgta ctcatcttcg
     2041 aacagcactt gcgcgggatg tccgtcgcga atgaaattct taggcaacac caagaaatcg
     2101 atttcgcccc ggccgagagc ttcccacggt tcattttgat tgccgatgag ttcgatggta
     2161 atccccggcg cctcgcgttc gagttgtcgc agcgccgccg gcatgagtac ggtggataca
     2221 tagtccgaca tcatcaacgt gaagttgcgc ttcgagttca acggatcgaa tttcgggcga
     2281 atatcgacgg tggcttgcac atgcagcaag caatctcgaa cactgccttc caggctctcc
     2341 cccaatggag tgggcaccat gcgccgcccg atttgcgtga gcagctcatc gtcgaagtaa
     2401 atccgcagac gcgccaaggc gccgctggtc gccgaagggc tgagattcag gcgctctgcg
     2461 gcgtgcgtga tgctgcgctc cgccagcagc gcatcgagcg caaccaacag attcaagtcg
     2521 aggcgattca gattcatgcc agccgcgcgt ctcgtgctac cggagcagct agcgcggcgc
     2581 gtcccgatga atggggtcga tccagtacac ctcctcaacc cgctcggcgg catcgatatt
     2641 caggttgacg accaccgcgt cattgccgct gcgcaccagc acgcattcga gcggcgtgtc
     2701 gctcgaccga ttgatctctt ggtgcggcac ataaggcggc acgaagatga agtcgccggc
     2761 atccgcctcg gcgacaaact cgagccgctc gccccagcgc atccgcgcaa tgccgcgcac
     2821 tacataaatg acgctctcga gcgcgccgtg atggtgtgcg ccggtcttgg cgttcggctg
     2881 aacgatgacc gtgccggccc agatcttttg cgagccgcgc gccgcattca ctgcggcggc
     2941 ccgcagcatg cccggagttt gcggcgtatt gctatccagg cgctcgctct tgatgacctt
     3001 gacgccgtcg atgcgccaat cgcgtggttg ggaatccgtc atatgtcagc ccgccagcgt
     3061 cctgatcagt acatcccagt aggtgagccc attatagaat tcgttgaccc ggatgcgctc
     3121 attgagcccg tgtgcgaatt cttcgctgtc catgatgaag actccgccga caccgtaggt
     3181 gggtatgccc gcgttgcgga acacggcacc gtcggtggca taggcggcct gggtcggcac
     3241 caccggcgcc ccgggatccg ccgcttggac cgctttagcg accgcattca tgacgtcggc
     3301 acgcatcggc gaggccgtcg acaccagcgc atcgtagccc tgcgtcacct gcacgttggg
     3361 tcccaccaga ccttgcaagg ttttctgcac ctccgcggca ctggtgcccg gaaatatccg
     3421 gcagttgatg ctggcgctcg ccgactgcgg cagcgcattc tcggcgtggc cgccgctcag
     3481 cagtgttgcc acgcaggtgg tgcggattcg gccgatgaag gccgggttct tggcgatctc
     3541 ggccgccgcc gccgcgttgc ctgggtccgc cgcgaaatgc gccatcgcct cgcccagcgg
     3601 acctttggtc accgcgctcg ccgccttgaa atcgccgacg gtccattcgt tccacttcac
     3661 cggaaactca tagcgttgca ccgccttgag cgcgtcggcg agttcataga tggcattgtc
     3721 ggtgcgcggc tcggagctat ggccgccggg attgtgtgtt gtcaattgaa attgagccga
     3781 gcttttctcc gcgccttgaa cgtagaaaat gagcggtttg gcactgccct cgctcaatac
     3841 gccgccgccg ccatcgccgt tgagggcaaa ttccgcatcg acgaggtcgc gatgcttggt
     3901 cacgagatcc cgcgcggtat cttgagcggt ctcctcatcg ccgctgaacg cgatgatcag
     3961 atcgcgcgtc ggcacgaagc cttgcgcctt gagccgcaag aattccgcgg tcaggagtgc
     4021 cacttcctgc ttgacgtccg aggtgccgcg gccgaagaag aatccctgtt cctcggtgag
     4081 atggtaagga tcgcgctgcc agtcgctgcg cttcgccgtg acgacgtcca tgtgggcgat
     4141 gaaatcgatc ggccgcccgc catggccgtt gccgcggtaa cgcaccacca aagaggcggt
     4201 ctcgcccagc ggcaggacgt ggatgtcggc cgccgggaaa ccgccggcct gaaatcgttc
     4261 ggcaaggtac ttggccacca gcgggacatt gcccttgccg atcgaactct cgataccgac
     4321 gatgtgggca aatatctcgc gcgcctgggc gcgcacggca tcgggcgcgg ccgcgggtgc
     4381 ggcgcttgcc gcaaaattcg cacccaagcc ggcggcacaa aagctcaaag atagccagag
     4441 tcgtccgatt attatcatgc atccccccgt agcgagcctg cgacagaacc tgcgagaata
     4501 accgaccgca tgcaatcaag cgcgcaaatt ccggtttgga cgatagcggc cccgattttc
     4561 gcagccgccg tcctgcttac ggccgcactc ggactcaccg ccggcgcact cgccgtcggc
     4621 ctgatcgccc tgggtctggg cggcagcgta tttgccgccg tccaccatgc cgaactcgtc
     4681 gcacatcgca tcggcgagcc ttatggcact ctggttctgg cgctggccgt cacgttgatc
     4741 gaggtcgcgc tgatcgtgtc gctgatggcc gcgggcggtg ccgaagcggc cggcctggcg
     4801 cgcgacaccg tattcgcggc cgtcatgatc atcctcaacg gcatcatcgg tctgtgcctg
     4861 ctggtcggcg gcatcaagta ccgcgagcag agcttcggct tggacggcgt gagcgcggcc
     4921 ctggtggcgt tgggtacgat cgtcatcttg actttggtat ttcccaatta caccgtgacc
     4981 acccccgggc cgttctattc tccgagtcaa ctcgcgctca ttgcggccgt aacactcgtg
     5041 gtctatggtg cattcatatt cacgcaaacg atcgagcacc ggccgctgtt tctcgacgac
     5101 gccaaaccgg cacaggccgc gcctgcggcc cggccgggca actggatcac cgtcatcagt
     5161 acgctgttgt tgctggcgtg cctcatcagc gtggtgctgc tggcaaagaa actggcaccc
     5221 agcctcgagg ccgccgtgat gcgcgtcggc gcgcccaacg ccgtggtcgg tgtaatcatc
     5281 gccgccatcg tcctgctgcc ggaaggactc gcggcgctgc aagccgctcg cggcaaccgt
     5341 ctgcaaacca gtctcaatct agccttggga tccgcgctcg cgagcatcgg gctgaccatt
     5401 cctgcggtca gtttcctgtc gctgatgacg aattctcact tgcgcttggg gttggattcc
     5461 aagtcgtcgc tgctgctgat gctgtcgctg ttcgtggtcg tcgtcgtgct gcgcaccgga
     5521 cgcaccatca tcctgcatgg aatcgtgttg ctggtcgtat tcgtcgttta cttgttcacc
     5581 acggtcgtgc catagctgat atttagctgc gcgcggcgac ttcgtcgata tgcttgagca
     5641 ggtcagccgg atcctcgtaa acgcgaaatg cgccggcgcg tgccaactct tcgtcgccat
     5701 agccgccgga gaggagtccc acaccgagtc cgcgtgcgcg ttgcgcggcg atcatgtccc
     5761 agatgctgtc gccgagcacc agcgaattgc gaatgtcgac gccgagacgc tcggccgccg
     5821 tcaagaacaa atccggatcg ggcttggcgt atttcacctg gtcgcgcgtg accacgggca
     5881 ccttggccgg gtcgactccg agcgccgcga gattgtgtcc cgcggtctcc atgcgtccac
     5941 tggtggcgat cgcccacggg gtgccttgcg ccgtgagata gtcgagcaac tcgcgcgcac
     6001 ccggcagcgg ccgcacgctg cccgagagcg cccggtaggc ctcggcatgg cgtacgcgca
     6061 gccgttccaa gcgctccggg gtattgtcga agcccgtctc gcgcaacagg atgttggtaa
     6121 acaatccgcc gctcatgccg atcttgcgat gaatgcgcca gacggacagg gggatgcctt
     6181 cctggtcgag cgaggatttc cacgccagca cgtgttggta aacgctatcg atcaaggtgc
     6241 cgtcgagatc gaagagaaac gcggtcttgt cgcgggccat gcgcacatta tgccgtgccg
     6301 cgttgccgcc tgtgcggcga ttgcgcacct ttacctgccc gggccccggg gattgggtaa
     6361 gatgggcggg gactttggga gcagcgatga aacacctctg gatggccctg ttcgtgcttg
     6421 cgttgaattt ggcggccggc accgtccagg ccgagaagct gcgcatcatc agctgggcgg
     6481 attacgtacc ggccgacctg attgcggcat tcaagaaaga aaccggcatc gacgtggaat
     6541 tgaccctgtc gaacaacgaa gagatcattt ccaagctgcg cgcaactggc ggcgccggct
     6601 acgacctggc gcaaccctcg caggatcgca ttgtgtccgc gcagcgcgaa ttcgggatct
     6661 acaagcccat cgatctgacg aaaatcaagc tcgaggagtt tcagccgcag ttcttggaca
     6721 tcgtccgcaa gaacaccagc ctcgacggca aagtctacgg gctgccttat ttgtggggca
     6781 ccgatggact cgtggtcaac gtgaagcgca gcaaaattgc cgactattcg gacttgtgcc
     6841 gcgccgacct caagggtaag accgccattc gcctgcgccg cccgacgttg atggcgttcg
     6901 cgttcgccgc gggcaaggac ccgttcgcgc tgtacgcgga tcccaaggcc tatagcgcac
     6961 tcatggacca ggtgggcgcc gcgctcatcg cctgcaaggc gaacttcaaa ttcttctacg
     7021 acaacaagga tcagttgctg aacggcattc gctccggtga ggtcgtggcc gccatgatgt
     7081 gggacagcgg cggctggacg ctcaaccgcg agaatcccga cattcaattc atcgtcccgc
     7141 gctccggcgc gctgggttgg ctcgacacct tcgccctccc cgcgcgtggc aagaacgacg
     7201 ccgctgccta tgcctggatc aactttgcga tgcggccgga aaacgcggct cgcgtggtca
     7261 agtcggtggg cagcttctcg accgcgatgc acacggatcc gctgatcgat ccgcgcctga
     7321 aggcgcaatt cgcgctcgct tttccgaaca acgacctgaa gagcatccac tggtatcccg
     7381 caatcccggc cggtctcgaa gaaatagaag gcaaggtgct cgaccgcgtg aaggccgcca
     7441 attgagcacg cccgacctgc tcgcggacgc cgtggtcaag cgttttgccg atcacaccgc
     7501 cgtcgattcg ctgtcgtttg ccgtggcgcg cggcagcttc ttctcgattc tcggcccctc
     7561 gggctgcggc aagacgactc tgctgcgaat gatcgccggc ttcatcgccc ccgacagcgg
     7621 cgacattgcc atcggcggcg cgagcatgcg cggcgtcgca ccgaatcgcc gcccggtgaa
     7681 catggtgttc cagcagctgg cgctgttccc gatgatgtcc gtgggcgaga atgtcgcatt
     7741 cggcctggcg cggcgcggta tcgcccgggc cgaacgcatc aagcgcagca atgcgatgct
     7801 ggagcgggtc gggctcggca acgcagccca caagcgcgtc gacgaattgt cgggcggtca
     7861 gcggcagcgc gtggcgatcg cgcgcagctt ggtgctcgag ccgacgctgt tgctgctcga
     7921 tgaacccctc ggggcgctcg atctgaaatt gcgcgagcac atgaaaatcg agctcaagca
     7981 attgcaggcg gccttcggca cgaccttcgt gtacatcacg catgaccaat ccgaggcctt
     8041 ggtgctctcc gatcacgtcg gggtgatgag ccacggacgc ttcgagcagc tgggtacgcc
     8101 ccaggacctg tactaccggc cgcaaacgcc gttcgtggcg ggcttcgtcg gtgcgaataa
     8161 tcgcatcgtg ggccgtgcca gcgcctccga cggcgcgctt gccgaagtca cttcggcgga
     8221 cggatggacg gtgcgtgccc tgaagtccgg cacgatcgcc gtcggcgacg cggtcgaggt
     8281 tttcgtgcgt ccggaagtcg caagtctggc gcgcaacgcc gcggaactgc cggccggcca
     8341 gccgcgccac agcggcgagg ttcagagcgt gctgttcgat ggcgcgaact cggcggtact
     8401 gctgcaggaa acgcagacgc ggcgcgaatt tcgcatcgcg ctgccccaaa ccgggcagtt
     8461 ctccgacctg aagccgcagg agaaagtggc gttcagtttc gacccgtcgc gagccgtatg
     8521 ctttgcggcg cgcgccgatg cgtgattcgc gctggcacgc gcgcctggta ctcggactgt
     8581 tgctcgcacc ggccgtgctc tggctcggcg cgctgattgt gttgccgcat gtcgacctgg
     8641 cagtgctgtc cttgcgcgag cgcgtcggac cgcgtcagta ctccttgagc ctcgcgcaat
     8701 atcggacttt cttcgccgaa ccgctctatt ggcatgtgtt cgtgcgcacg gcggtgatgt
     8761 cggtgctggc gactttcctg actttgctgc tggcctttcc gattgcatgg atcattgcaa
     8821 aattggcgaa aggccgtgcc ggctcgctgc tgttcattat ttgcctgatc cccttttggg
     8881 tgagcgaaac cgtgcgcacc ctcggctgga tgatcttgct gcgcgaatcc ggtgttctgc
     8941 ccgcactgat ggtcaagctc ggactcaccg cgatgccggt ggagctgctg tatcgcgact
     9001 ccaccatcct ggtcggactc gtgtacacct cgttgctctt catggtcgtg cctctggtct
     9061 cgagcctcga aagcctggac aactcgctga tcgaggccgc ctacgatttg ggtgccggcg
     9121 gatttaccat cttgcgcagc atcgtgatcc cgcatgccgc tcccggcatc accgccggct
     9181 gcatcgtcgt gttcatgctg acgctcggca attacctgac tccgacgctg ctcggcggca
     9241 agaattcgca atggttcacc gagcagatct acacgcagtt cattacgcgc ttcaattggg
     9301 aacagggtgc cgcattcggc ttcctgttgc tcggactgtc caccgcgctg gtaatcctcg
     9361 gcttgaggat cagcggccag cgattcggcg acgtgatgcg cgccacatga tcccctcgct
     9421 gccgcgcccg gcctggctgc gcgccgccac cgtggcgtac ttgatcgcgt ttctgctgtt
     9481 cctgtttttg ccgctggcgg tcgtcgcggt gttcgccttc aacaacgccc cgtatcccgc
     9541 gcctccgtgg cgcggattta ccctcgactg gtttctgggc aacgccgctc tcggacgcac
     9601 cggcctgttc gccgacagcg aactgctcgg cagcatctgg accagctgcc tggtcgccct
     9661 gtgcgtcacg gcgttgtcgg cggtcgtggg catcagcaac tcgttcttga tcgagcgcgg
     9721 ccgctttccc ggcaagcagc tgttgtcgat cttgatgctg gcgccgttgg tgatacccgg
     9781 cgtgatcctg ggcatttcga ttctcgcctt cgccagccga atggcggatt tcgcggatga
     9841 caccctgggt ctggagcttg attttctcag gcccggcctg ccgttggtgg tgctcggtca
     9901 attctcgtac atcgcgacca ttgcaacatt gaccatcagc gcccgattgc ggcgcttcga
     9961 ccgcaccttg gaggaagcgg cgctgaatct tggcgcctcg aaggcggccg tgtttcgcac
    10021 catcttgctg ccctacctgc ggccggcgct gctcgggtcg gccgcgatct cgttcttgat
    10081 gtcgttcgcg gatttcaata ccacgctgat gttggtcggt gcggacacgc cgttgacggt
    10141 gatgatgtac ggccgcatgc gcgaaggcgc gaccccggtg ctgaatgccg tcagtctgtt
    10201 tctgatgctc gcgtcggccg tgttggcgct cacactgctg cgccgctcgc cgcgcccgca
    10261 agccgcgccg tgacgcctat ttagctcgcg gcctagctac gctgcggcac cgagaatctc
    10321 gaccagctgc acccgccggt ccgaggtcgc ggattcgaat tcggaactga tggtcgtcaa
    10381 ctcctcgtcg tcgcgggcgc tcaccagctt gatgaggcag gcgcgatcgc ggccgcggcg
    10441 cttggcctca tacagcgcct tgtcgactag gctgatggcg gcctcgaggg aaatgtcggc
    10501 cgccgaaccc atcatcggga aacacgcgta gccgattgaa atcgtgcagt ggatgacctg
    10561 tccgttccac accaccggct cgcaacgcac ggcgtgcagc agccgcgcag cggtcaaatc
    10621 cgcctggttc ggcgacatgg gtccgagcac cgccaggaac tcctcgccgc cccagcgcac
    10681 cagtttgtcg ttctcgcgca gcgccgcggc gagccgttgg ctcatcgcgg ccaagacggc
    10741 atcgccggcc ggatgcccga aggtgtcgtt gatgcgcttg aagtgatcga gatccgccag
    10801 caatatgcag ccgcccaccg gccggccgcc gtcgaccgcg agcacacgct cgttgaaata
    10861 gcgccggttc gacaatccgg tgagcgggtc atgctcgctg ctgaagcgca gccgctcgtt
    10921 ggctttgctc acgcgggcga acgcccacac gagcagcccg caaatgaagg cgatgaacag
    10981 cgcggccgcc aggatcaatt gctggcgcag gcgttgtccg cgcatttccg agactttgag
    11041 tgcattgtca cgccgcaaca gctcgagctc gcgcgccttg cgttcatcgt cgaattgcgc
    11101 cgagagttcc aagaaagcac gctgccgcgt gttcgtcatg aacttttcgc tgaccgcctc
    11161 gtaacggtgg taaacctgaa tcgccatcat caagtagccg gcgtgttcca gggcatcggc
    11221 atactcgcgc agcgattcct tcgcgtccag catgttgctg ccggcaagcg tgtcgccgat
    11281 ggctcgttcg gcgagctttt cgcccatctt gatgctgccg agaccgatgt aagcgagacc
    11341 ctcgttgaac tggatgatcg tctcatcgtc gcgctgattg gtgcgctgga tgatcggcat
    11401 cgcttccttg gaatacttga gcgattcggc gtaatcgcgc gttttcaaat aagaatcacc
    11461 cagattgagc agcgccaacg cgagccactt gctgctctcc gagcgtttcg catgggccaa
    11521 gccggccaac gaggcgcgcc gctcctcggc ccgatcaccg cgccggtccg caatgtcggc
    11581 tttgcgttcc tcggtttcca ccagcgaggt ttcatcgccc aatcgagtcg caagactcat
    11641 ggcggcgttc aattgcacgc tcgcccggtc gaagttgccg ctgtcggtgt aaatgtgcgc
    11701 cagcgacagc atcgcatgca gggttcgcaa gtcatcgcgc atcttgccgg caagatccaa
    11761 cgcctgttcc aagaaaggta gcgccgcctc gtcctgaccg agaattcgca gcacatggcc
    11821 gcgcagcgtc agcacgcgat agcgctccgt atcggacacg atcttgctga tgtcgatgcc
    11881 cttgagctcg gcttcggcgg cggcaaactg gttgtgctgg cgggcggaat acgcgcgcac
    11941 gaatccgccg gcgcggactg ccgcatcatt gcctgtgccc gcgatggctt cgaggcgccg
    12001 ctcgaccgca tagacgtctt cgtcgcgcgt gctgtccgcg tagatcatgc cgcgaatttc
    12061 cagcatctcg atttcgtcgg cgacggaact cgacgagggc ggcggtgcgc cgtcgagcat
    12121 ctgtgcagcg cgctcgggat cggaccactc cagcatctcg agctgactgg cgagagtcga
    12181 ctccgcggcg cgcacggatg cagtcgcggc gcagagcaca agcgccccga ttaccgcgtg
    12241 aatgttaact tttcgcatat ccggccagtc ttggaccgcg gtgcaacacc atagactgca
    12301 tatttgccac gattggctgg aaagtgacgt gatgaaggtc atctctcaag tgtcagctaa
    12361 ggctttgcct taagcttggc tgccaatagt gtgacgcgcg tcctcgcggc agcacggttc
    12421 agggcgcgat ttgcgcctca gtcatcgacg gccgaataca ccagagcgcg gaacaaggtg
    12481 cggtagtagt cgcgctgccc cggagcctga atcaacagca ccgcgaacag ctgctccgcc
    12541 ggatccaccc agaatgtggt gccggcgagg ccgccccaaa aatactggcc gatggtcccc
    12601 ggtacgtgcg cgatgccggt gtgtagacga acggcaaatc cgagtccgaa accatggccc
    12661 ggcagcagca gatcgggcgc tccggtcacc ggcccgaggt ggtccgccgt catcagttcg
    12721 agggttttgc ggctcaagaa tcgctgtccg tcgaatttcc cgagatcgag cagcatttgc
    12781 aggaagcgcg cgtaatcgcc ggcggtcgac accaggccgc caccgccgga ctcgaacttc
    12841 ggcgcatcgc gcacttccag cagccgtacc gcgaggccgg tgtcgggatc cgtaggccac
    12901 gcctcggcga ggcgcgagtg gtaccgctcc gggacgtgga aagcggtgtc gaccatgccg
    12961 agcggcgtga agatgcgctg ttcgaagaac tcgcgcagcg tctgcccgga taacacctcg
    13021 atcagccggc cgatcacatc ggtcgagcgg ccgtattccc agcgcgttcc cggctgatgc
    13081 agcagcggca gcctgcccag ggtcgcgact tgttcggcat tgctctgcgc gcggcgggcg
    13141 attttggcgg ccgcgtacat tttttgcacc gcaccgggac cgcggaattc gtaggtgaag
    13201 ccggacgtat ggcgcaatag atcctggatg gtgatgtcgc gttcgagggg cacccgttcg
    13261 aacatgccgc cgtggccgat cgcgaccttg aggtccgcca gctcaggcag gtacttgacg
    13321 atgggatcgc tcagcaggaa cttgccctct tcccagagca tcatggccgc gaccgacgtg
    13381 atgggcttgg tcatcgaata gattcggaag atcgaatctg gggtcatcgg cgcggcgctc
    13441 gcgggatcgc gttgaccgaa gctttcgaag aatgcgatgc ggccgcgccg cgcgatgagc
    13501 gcgacggcgc ccggtacgcg tccgcgctga acttccgcgc tcatcaccgc ggaga
//
LOCUS       DASWZT010000319         6989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_31676, whole genome shotgun sequence.
ACCESSION   DASWZT010000319 DASWZT010000000
VERSION     DASWZT010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6989
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31676"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1493)
                     /locus_tag="VGE92_08785"
     CDS             complement(<1..1493)
                     /locus_tag="VGE92_08785"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY1313961.1"
                     /translation="MTNPTADERVNVAPARNDDKTLIRGFTNAPPLTAQAGHLDTDAL
                     PLNVSALPLDLDALPPHTNSDWVEPDPQAAGAETTVGSLLGNRYLLEYKVGEGGMGVV
                     YRATDVEVKGEIFAVKVLKPAIQQRPEALKLLREEVRKTRGLKHPNIVGVYSLNSDPS
                     GAYMLMEFLEGKPLSALIDEEFGRGMPLMRAWPLINDIGSALAYAHDHNVIHSDLKPS
                     NLFVTVSGKAMLLDFGIARAVRARADRFDTGSLGALTPAYATSEMISGNAPDQSDDVY
                     SFACVIYEMLSGKHPFNSLGAVDAKRVGSQLMPLPSLTHRQNQALAHALAFERANRTP
                     SVEALLAGLRGPGRRPAPPRWIWPAVGAGACLLAGTVAWLMWRGRAPEHAQNAAATPA
                     VVSPSRAVPPAPNKGAVALDASSQKSRDKTAVAAATSPTPPLRETPSPAIAAAPSLAN
                     PVHRPAVPDAKPLPLPAVKQSTTPPAEGAAPAPAAAPESTPSSGSAMP"
     gene            1786..3732
                     /locus_tag="VGE92_08790"
     CDS             1786..3732
                     /locus_tag="VGE92_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313962.1"
                     /translation="MSRRIYDDTRTEQDGDGGMAEGLIGGILGEEDEKPEAEAAEAVP
                     GAEAFAAAIVAIASRQDPQVARETSIFLGKQSRLLDIQAKHLEEEHALRLGHLRGQKY
                     EGTLRRAGIRIRIAFQLFTAIVAALFAAGLVLLVRDAITSRSVVIDPVDVAPNIAAHV
                     PSDKIVAAGLLDVLAQIQAATRTSAERRSLSNAWTSEIEIEVPETGVSIGQLERILKT
                     RFGHDTHIDGDLVQTEKGGLALTVRGTGILPKTFSDAGSNLEKLTTEAGEYIYSQSQP
                     GLWMNYLVNNGRNDEAIRFAGANYNAVNASERPFVLNYWANAISASGGPAATAEALPL
                     WRETVRLKPDFWTGYNNVLLALVELGREEETIAVVEQIMKIAGGRPGRAPEITYQNYD
                     QLVFDIPAMYASNLADLESHPGIGTSTAYSGALNLSIAQIEVQMHDLAAAQIRLKTIR
                     VDEKVLPDVASAALTRASLAEERGDLTTASAEWDVFRTAAVDPGYLAANGVYICYSAV
                     TYQKTGQPQKADAALSKLEPLTLVDCDRFRGDVLDLRGDWVGAQAWYAKAAQLAPSLP
                     AGYFSWGVALAKHGDLAGAVAKLQLAHQKGPHWADPLKAWGDVLLKQGKAQDALAKYD
                     EALKFAPNWSELQAARDALANHKS"
     gene            complement(3764..5302)
                     /gene="hutH"
                     /locus_tag="VGE92_08795"
     CDS             complement(3764..5302)
                     /gene="hutH"
                     /locus_tag="VGE92_08795"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEY1313963.1"
                     /translation="MTAILQIRAGELSLATLRRIYEQPVALKLSADDRGRIAVASSLV
                     DKIIAAGDAAYGINTGFGLLAQTRIPTEQLELLQRNLLLSHAAGVGDALPDAVVRLIL
                     ALKVNALARGHSGITMAVIDALLALLEHEVYPVIPAQGSVGASGDLAPLAHLSTVLLG
                     IGQVRVRGAVLPAAEGLGLAGLGVLKLRAKEGLALINGTQVSTALALAGLFGAEDVFA
                     AALIGGAMSVDALKGSDSPFDPRIQEVRGQPGQIAVAREYRDLIAGSAIRASHVDCTR
                     VQDPYSFRCQPQVMGACLDLIRQCSVTLGIEANAVTDNPLLFVEAGEVLSGGNFHAEP
                     VAFAADTLALALAEIGSLSERRVAVLVDPKMSGLPAFLVENSGVNSGFMIAQVTAAAL
                     VAENKTIAAPCSVDSIPTSANQEDHVSMATHGARRLRNMVDNAAAVVGIELLAAAQGI
                     EFHRPARSSPSLEQVHASIRSDVPGYAADRYFAPDIQAAQQWVKTGRFSPLVKRILPS
                     RAGA"
     gene            complement(5302..6972)
                     /gene="hutU"
                     /locus_tag="VGE92_08800"
                     /pseudo
     CDS             complement(5302..6972)
                     /gene="hutU"
                     /locus_tag="VGE92_08800"
                     /inference="COORDINATES: protein motif:HMM:NF036634.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
BASE COUNT         1332 a         2195 c         2254 g         1208 t
ORIGIN      
        1 ggcatggccg acccgctgga tggcgtcgat tcgggcgcgg cggcaggagc gggggctgca
       61 ccttcggcgg gcggggtcgt cgattgctta acggcgggta aggggagcgg cttcgcgtcc
      121 ggtacggccg ggcgatgtac aggattggcg agcgagggcg cggcagcaat tgccgggctc
      181 ggcgtctcgc gtagcggcgg ggtcggggaa gttgccgctg caacggctgt tttatcgcga
      241 ctcttctgcg acgaggcgtc gagagccact gcccccttgt tgggagccgg cggcacggcg
      301 cggcttgggc tcaccaccgc gggcgtcgcc gcggcatttt gcgcatgctc cggcgcgcgg
      361 ccacgccaca tcagccaggc gaccgtgcct gcgaggagac atgcgccggc gcccaccgca
      421 ggccaaatcc atcgcggcgg cgccggccgc cgcccggggc ctcgaagtcc cgcgagcaac
      481 gcctcgaccg aaggcgtgcg attcgcgcgc tcgaacgcga gcgcgtgcgc cagcgcctga
      541 ttctgacggt gggtcaagga gggcaggggc atcaactgac tgccgacgcg cttcgcatcg
      601 acggcgccga ggctgttgaa aggatgcttg ccggacaaca tctcgtaaat gacgcacgcg
      661 aaggagtaca catcgtcgct ttgatccggc gcattgcccg agatcatttc ggaggtcgca
      721 tacgccgggg tcagcgcacc gagcgatccg gtatcaaacc ggtcggccct ggcgcgcacg
      781 gcccgggcaa tgccgaaatc aagcagcatc gccttgcccg acaccgtcac aaaaaggttc
      841 gacggcttca ggtcgctgtg gatgacgttg tggtcgtgag cgtaggccaa ggccgaaccg
      901 atgtcgttga tcaacggcca cgcacgcatc agcggcattc cgcggccaaa ttcctcatcg
      961 atgagggcgc tgagcggctt gccctccaga aattccatca gcatgtaagc gccgctcgga
     1021 tcggagttca gcgagtacac gccgacgatg ttgggatgct tcaggccgcg cgttttgcgc
     1081 acttcctcgc ggagcaattt cagcgcttcc gggcgttgct gaatcgcggg cttcaacacc
     1141 ttgacggcaa agatctcgcc cttgacctcg acatccgtcg ctcgatacac cacgcccatg
     1201 ccgccttcgc ccaccttgta ctcgagcaag tagcggttgc ccagcaagct gccgacggtg
     1261 gtctccgctc ccgccgcctg ggggtcgggt tcgacccagt cagaattcgt atgcggcggt
     1321 aaggcgtcga ggtccaacgg cagcgcgctg acattcaacg gcaacgcatc ggtgtccaag
     1381 tgccccgcct gcgcggtcag cggcggcgcg ttggtgaagc ctcgaattag agtcttgtcg
     1441 tcgttgcgag ccggtgccac attgacccgc tcgtcggcgg ttggatttgt catagcaatc
     1501 ggatcatacg ctgcccggcg caagaggtgg ttggaagccc ttcggggcgt taaatcatgg
     1561 attctggtcg gatgaaagta cagcggtaaa ttcgccgcgg gcgtgcgatg aggaattcgc
     1621 tcggcctaat ctcagccgcc cggcagccac gaattgccgg acataaatca ttgatttata
     1681 ggtgctagca gcacactgcg acttccaacg tcaactaata agtcaaatat tgaacctggg
     1741 tcagtcgtgg tcgcgtgcgc gacgcgtgca gcgacgctgg ttgcgatgag cagacgcata
     1801 tacgacgata cccgcacaga acaagacgga gatggaggga tggcggaagg cctgatcggt
     1861 gggattctgg gggaagagga cgagaaaccg gaagccgagg ccgcggaggc cgtgcccggc
     1921 gccgaggcct tcgcggcggc aattgtagcc attgcctctc ggcaggatcc gcaggttgcg
     1981 cgggaaacct cgatattttt aggcaaacag tcgcgactgc tcgacattca ggcgaagcat
     2041 cttgaagagg agcacgcgct gcgcttgggg catttgcgcg gccagaaata cgagggcact
     2101 ctgcgccgcg ccggcatccg tattcgcatc gccttccaac tgttcaccgc gatcgtcgcc
     2161 gctttgttcg ccgcgggatt ggtcttgctg gtgcgcgacg cgatcacctc gcgcagcgtg
     2221 gtaatcgacc ccgtcgacgt cgcgccgaac atcgcagccc acgtccccag cgacaagatc
     2281 gttgcagccg gtctgcttga cgtcctcgcg cagatccaag ccgcgactcg cacgagcgcc
     2341 gagcgccgtt cgttgtcgaa cgcctggacg agcgagatcg agatcgaggt gcccgagacg
     2401 ggagtgtcga tcgggcaact ggagcggata ctgaagacgc gattcgggca tgacacccac
     2461 atcgatggtg atctggtaca gaccgaaaag gggggactgg cgctcaccgt gcgcggtacg
     2521 gggatactgc ccaagacctt cagcgacgct ggcagcaacc ttgaaaagct caccaccgaa
     2581 gcgggcgaat atatctacag tcagagtcag cccgggctat ggatgaatta cctcgtaaac
     2641 aacggtcgca atgatgaagc tattcgcttt gcgggggcca actacaacgc ggtgaatgcc
     2701 agtgagcggc cctttgtcct caactactgg gcgaacgcca tctccgcgag tggcggtccc
     2761 gcggcgaccg ccgaggcgct gccgttatgg cgggaaaccg tgcgcctcaa acccgatttc
     2821 tggaccggat acaacaacgt cctcttggcc ttggtggagt tggggcgcga ggaggagacc
     2881 attgcggtcg tcgaacagat catgaagatt gccggcggac gaccgggaag ggcgccggag
     2941 attacgtatc agaattacga ccagttggtt ttcgacatcc ccgccatgta tgcgagcaac
     3001 ctcgccgatt tggagagcca ccccggcatc ggcacgtcaa ccgcgtactc gggcgcctta
     3061 aatctgtcga tcgcccagat tgaagttcag atgcacgacc tggccgccgc gcagattcga
     3121 ttaaaaacaa tccgggtcga tgagaaagta ttgcccgacg tcgcgtcggc cgccttaacg
     3181 cgcgcgtcgc tcgccgagga acgcggtgat ctcacgaccg ccagcgcgga gtgggatgtc
     3241 ttcaggacgg ccgcagttga cccgggctac ctcgcggcga atggcgtgta catttgctat
     3301 tcggcggtga cctatcaaaa gacggggcag ccgcagaagg cggacgcggc cctaagcaag
     3361 ctcgagccgc tgacgctcgt cgattgtgac cgcttccgcg gcgatgtgct cgacctgcgc
     3421 ggcgattggg tgggggcaca ggcgtggtac gcaaaggcgg cacaactcgc tccgagcctg
     3481 ccggcgggct atttctcgtg gggcgtcgcc cttgcgaaac acggggatct cgccggggcg
     3541 gtcgccaagc ttcagctcgc tcaccaaaaa ggaccgcatt gggcggaccc tctgaaagcc
     3601 tggggcgacg tgctgctcaa acaaggaaag gcgcaggacg cgcttgcgaa atacgacgag
     3661 gcgctcaagt tcgcgccgaa ctggagtgaa cttcaggcag cgcgcgacgc cttggcgaat
     3721 cacaagagct gattgggcga agcggggtaa ttgggcaaat tcgtcaggcg cccgcgcgcg
     3781 acggcaagat gcgcttgacg agcggcgaga agcgtccagt cttgacccac tgctgcgcgg
     3841 cctggatgtc cggtgcgaaa taccgatcgg ccgcgtaacc cggcacatcg ctgcggatgc
     3901 tcgcgtgaac ctgctcgaga ctcggcgaag agcgtgcagg ccggtgaaac tcgatgccct
     3961 gtgcggcggc cagcaattca atgccgacca cggccgccgc gttgtcgacc atgttccgca
     4021 gccgacgcgc gccatgggtg gccatgctga cgtggtcttc ttgattcgcc gacgtcggaa
     4081 tcgagtcgac gctgcacggg gcggcgatgg ttttgttctc ggccaccaag gctgcggcgg
     4141 tgacctgcgc gatcatgaaa ccggaattca cgccgctgtt ctcgaccaag aatgccggca
     4201 gaccgctcat tttcggatcg accaagacgg ccacgcgccg ctccgacagg ctgccgattt
     4261 ccgccagcgc gagcgccagc gtatcggccg caaaggccac gggctcagca tggaaatttc
     4321 cgccggagag aacctcaccc gcctcgacga acagcaaggg attatccgtg accgcattcg
     4381 cctcgatgcc gagcgtcacg ctgcactgac gaatcagatc gaggcaggca cccatgactt
     4441 gcggctgaca gcggaaggaa tacgggtcct gcacccgggt gcaatcgacg tgcgaggcgc
     4501 gaatggcgct gccggcgatc aagtcgcggt actcgcgcgc gacggctatc tgcccgggct
     4561 gcccgcgaac ctcctggatg cgcggatcga acggggaatc gctgcccttg agcgcatcca
     4621 ccgacatggc gccgccgatc agtgcggcgg caaacacatc ctcggcgcca aaaagtccgg
     4681 ccaaggcgag cgcggtcgac acctgcgtgc cgttgatcaa cgccaggccc tctttggcgc
     4741 gcagcttgag cacgccaagt ccggcgagcc caagtccctc ggccgcgggc agcacggcgc
     4801 cgcgcacgcg tacctggccg atgccgagca acaccgtact caagtgagcg agcggcgcca
     4861 gatccccgga ggcaccgacc gagccttgcg cgggaatgac cggatagacc tcgtgctcga
     4921 gcagcgcaag cagggcatcg atcaccgcca tggtgatgcc cgagtgtccg cgcgccaggg
     4981 cgtttacttt aagcgcgaga atcaaccgca ccacggcatc gggcagcgcg tcgccgaccc
     5041 cggccgcgtg cgacaacaac agattgcgtt gcaaaagctc cagttgttcg gtagggatcc
     5101 gtgtctgcgc caacagcccg aagccggtgt tgatgccata ggcggcatcg ccggccgcga
     5161 tgatcttgtc gaccaaggaa ctcgccacgg caatgcggcc gcggtcatcg gccgacagct
     5221 tgagcgcgac cggttgctcg tagattcgcc gcagcgtcgc gagcgaaagc tcgccggcgc
     5281 gaatctgcaa gatagcggtc actacgggtg ctcgatcatc ggcaggttga gcccctgttc
     5341 gcgggcgcag gcgatggcaa tgtcatagcc ggcatcggca tgacgcatga ccccggtgcc
     5401 cggatcgttc cagagcacgc gacgcactcg cttggccgcc gcctcgctgc cgtcgcaaac
     5461 gatcacgaca cccgcgtgct gtgaatagcc catgccgacc ccgccgccgt gatgaatcga
     5521 cacccaggtc gcgccgctcg ccgtattgag cagcgcgttg agcagcggcc aatcggagac
     5581 cgcgtccgag ccgtccttca tcgcctcggt ctcacggttc gggctcgcca cggaacctga
     5641 gtccaagtgg tcgcggccga tgacgatggg cgcggacagt tcgccgctct tcaccatctc
     5701 gttgaacgcc agcccgagac gatggcgttg ccccaaaccc acccagcaaa tgcgcgccgg
     5761 caatccttgg aaatgaattt gcgagcgtgc catgtcgagc caatgatgca aatgcggatc
     5821 atcggggatc agctctttga ccttggcgtc ggtcttgtag atatcttcgg ggtcgcccga
     5881 aagcgcgacc cagcggaagg gtcccacgcc gcggcaaaac agcggccgga tgtactcggg
     5941 cacgaaaccg ggcacatcaa aggcgtgagc aacgccttca ttgagggcaa cttggcggat
     6001 gttgttgccg tagtcgaaga ccggcagccc catgcgcttg aagcgcagca ggtattcgac
     6061 gtgcttggcc atcgagcgat ttgcagcgag agtagtgccg gcgggatcgc tcgcacgccg
     6121 ttcgtcccac tgctcgacgg tccagccctg cggcaggtag ccgttgaccg gatcgtgcgc
     6181 cgaggtctga tcggtcacgg catccgggcg gatcccacgt tccagcaggg tgtgcaggac
     6241 atcgacgata ttgccgagga gcgcgaccga gataggtttg gatttcttct tcgccgcatc
     6301 gatcagcgcg agcgccgcat cgagcgaatc ggcttgcgcg tccacatacc cggtttccag
     6361 gcgcttcgcg atgcggctct gccggcactc gaccgccaac atgctgaacc cggccatggt
     6421 cgccgccagc ggctgcgcgc cgcccatgcc accgagtccc gccgtcagta tccatttgcc
     6481 gttgagctga tcgttgaagt cccgccggcc catttcggca aaggtttcgt aggtgccttg
     6541 cacgatccct tggctgccga tgtacaccca ggaacccgcg gtcatctggc cgtacatggc
     6601 aagacccttg cgatcgagtt catgaaaatg ttcccaggtc gctcaatgcg gcaccaaatt
     6661 cgaattggcg atcagtaccc gcggcgcgtc cgcatgcgtc gggaatacgc ccaccggctt
     6721 gcccgattgc accagcagag tctcttcttc ggtcagcgtc ttcagcgtct cgacgatgcg
     6781 gtcgaaacaa ttccaatcgc gcgcggcgcg gccgatgccg ccgtacacca ccagttccgc
     6841 gggattttca gcaacttccg gatccaagtt gttcatcagc atgcgcagcg gcgcttccgt
     6901 catccaggta cgcgccgtga gcgccttgcc gcgggcggaa cggatgactc ggggttttac
     6961 gggggcaagc atggttacat cctcagggg
//
LOCUS       DASWZT010000320         3631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_31683, whole genome shotgun sequence.
ACCESSION   DASWZT010000320 DASWZT010000000
VERSION     DASWZT010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31683"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /gene="dnaN"
                     /locus_tag="VGE92_08805"
     CDS             complement(<1..488)
                     /gene="dnaN"
                     /locus_tag="VGE92_08805"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEY1313964.1"
                     /translation="MKVTVERAELLKSLGHVHRVVERRNTIPILANVLIKAERGKLSM
                     KATDLDLEIIDSVAAEVSPGGATTVPAHMFYEIVRKLPEGSQIVIEGSGDRAVLSLRA
                     GRSRFTLQTLPESDFPDLAAGEMTHSFKLAASDLKRLIDKTQFAISTEETRYYLNGIY
                     LHT"
     gene            complement(701..2128)
                     /gene="dnaA"
                     /locus_tag="VGE92_08810"
     CDS             complement(701..2128)
                     /gene="dnaA"
                     /locus_tag="VGE92_08810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEY1313965.1"
                     /translation="MSNADQDGWSRVKGRLRAEVGDDVYSSWFARMDLEAIEEGTVRL
                     SVPTRFLKSWIQSHYAERVLACWQVEQNQVGRIELIVRSAVLRSAIAKPKPELETGAG
                     DGIRFNSQNGNGRVLPASDATGHEALGGSPLDARLTFDSFVVGRSNTLAQAAAKQVAA
                     AKRGDAVMFNPLYIHAGVGLGKTHLLQAVTWSGNANGERRVLYLTAEKFMYGFVSALR
                     TQTALAFKEALRGIGVLVIDDLQFLQGKSTQAEFCHTLNALIDAGRQVVIAADRPPSD
                     LESLDDRVRSRLAGGLVVEIGPLGEELRLEILKSRVTAARSHHPSFDVPLPVLGYIAK
                     TVTHNGRDLEGALNRLLAHNKLTGHAVTLEMAEREVRDLIRPQEPKRVKIEDIQRVVA
                     RQYNVSRSDLLSSRRTANVVRPRQVAMYLAKTLTLRSLPEIGRRFGGRDHTTVLHAVR
                     KIEGLVGNDMALAEEIEILKRQLQE"
     gene            2234..2680
                     /locus_tag="VGE92_08815"
     CDS             2234..2680
                     /locus_tag="VGE92_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313966.1"
                     /translation="MFREIFVPPGLKAGQNDTRIENAVPQPKGGGVAARLSGYDSETK
                     RAAVGRELALDDCRPTDAVFSAEARPATTMIVPPNCLPGLHPVVRFALRIVVLAAGGQ
                     TKAVLIRDEREAKPCFEVARDFRALELKTTQALSACNENRDCPHAT"
     gene            complement(3131..3397)
                     /gene="rpsT"
                     /locus_tag="VGE92_08820"
     CDS             complement(3131..3397)
                     /gene="rpsT"
                     /locus_tag="VGE92_08820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY1313967.1"
                     /translation="MANTKSARKATRKIARRTIVNKARRSVLRNSVRKIEEAIASGDR
                     NAALAAFKEAEPVIMRSAQRGVVHKNNASRKVSRLSHRIAKLAK"
BASE COUNT          682 a         1123 c         1135 g          691 t
ORIGIN      
        1 gtgtgcagat agatgccgtt gagataatag cgcgtctctt cggtcgagat cgcgaactgg
       61 gtcttgtcaa tgagccgctt caagtcgctg gcggcgagct tgaacgaatg cgtcatctcg
      121 ccggcggcca gatcgggaaa atcgctctcc ggcagcgtct gaagcgtgaa gcgcgagcgg
      181 ccggcacgca gcgacagcac agcgcggtcg ccggagcctt cgatcacgat ctgcgagccc
      241 tcgggcagct tgcgcacgat ttcgtagaac atgtgcgcgg gcaccgtggt ggcgccgccc
      301 ggcgacacct cggctgcgac gctgtcgatg atttccagat cgaggtcggt ggccttcatg
      361 ctaagcttgc cgcgctcggc cttgatcagc acgttggcga gaattggaat ggtattgcgc
      421 cgctcgacca cgcgatgcac gtggcccagc gacttcaaca gctctgcacg ctcgacggtg
      481 actttcatgg agagacccgc ttcggcgcct ttgaaaaggc ctttaatcta ggcgcgacgc
      541 caagccggcc gctcgcggaa tgcggggccg gggagcaaag gtgacaagag cgggccaaat
      601 gcgcaagggc gcgtttttac tccccgctca tatccccagg gcgtccacaa cccttgatcc
      661 gtcaggcccc gcataagccg ggcatgacgg ggaggtgcgg ctactcctgc aactgccgct
      721 tcaaaatctc gatttcctcg gccagcgcca tgtcgttgcc gaccagaccc tcgatcttgc
      781 ggaccgcgtg tagcacggtg gtgtggtcgc ggccgccgaa cctgcggccg atctccggca
      841 gcgaacgcag cgtcaaggtc ttggccagat acatcgccac ctgccgggga cgcaccacat
      901 tggcggtgcg gcgcgacgac aggaggtcgg accggctgac attgtactgg cgcgccacca
      961 ctcgctggat gtcctcgatc ttgacccgct tgggctcctg cgggcggatc aggtcgcgca
     1021 cctcgcgctc ggccatttcg agcgtgacgg catggccggt cagcttgtta tgggcgagca
     1081 ggcggttgag cgcgccttcg agatcccggc cgttgtgggt gacggtcttg gcgatatagc
     1141 ccagcaccgg cagcggcacg tcgaagctcg gatggtgcga gcgcgccgcg gtcacccgcg
     1201 atttgaggat ttcgagccgc agttcctcgc caagcggacc aatttccacc accagcccgc
     1261 cggccagccg cgagcgcacg cggtcgtcga gactctcgag gtcggacggc ggacggtcgg
     1321 ccgcgatcac cacctgacga ccggcgtcga tcagcgcatt gagcgtgtgg cagaactcgg
     1381 cctgggtcga cttgccttgc aggaattgca agtcgtcgat caccagcacg ccgatgccgc
     1441 gcagcgcctc cttgaaggcc agcgccgtct gggtccgcag cgccgagacg aagccgtaca
     1501 tgaacttctc ggcggtgaga tagagcaccc tgcgctcgcc attggcattg ccgctccagg
     1561 tcaccgcctg aagcagatgc gtcttgccca agccaacgcc ggcatgaatg tagagcggat
     1621 taaacatcac cgcgtcgccg cgcttggcgg cggccacctg cttggcggcg gcctgggcca
     1681 gcgtattgga gcggccgacc acgaaactgt cgaaggtcaa ccgggcgtcg agcggcgagc
     1741 cgcccagcgc ctcatggccg gtcgcgtcgc tggcgggaag aacccgcccg ttgccgttct
     1801 ggctgttgaa gcggatgccg tcgccggcgc cggtctccaa ctcgggcttc ggcttggcta
     1861 tggccgagcg cagcaccgcc gagcgcacga tcaactcgat gcggccgacc tggttctgtt
     1921 ccacctgcca gcaggccagc acgcgttcgg cgtagtgcga ctggatccag ctcttgagga
     1981 aacgggtcgg caccgacagg cgcacggttc cttcctcgat ggcttccaga tccatgcgcg
     2041 caaaccagct cgaatagacg tcgtcgccaa cctcggcgcg cagacgcccc ttcactcgtg
     2101 accagccgtc ttgatccgcg ttcgacattt tttcttactt ctctatgggc tcgattggtt
     2161 gagatgaact cgggttgctc ggcctggcgg ggtgagaccg ccgacgcacc gggaacggac
     2221 acaacaacac tcgatgtttc gggagatttt cgtgccgccg ggcctcaagg ccgggcagaa
     2281 cgacacgcgg atcgaaaatg ctgtgcctca acccaagggc ggcggcgtgg cggctcgatt
     2341 atcggggtat gattctgaga caaagcgggc ggcggtcggg cgtgaactcg cactggacga
     2401 ttgccgtcca acggacgctg ttttctccgc cgaagctcgt cccgccacta ccatgatagt
     2461 ccccccgaat tgcctgccgg gcttgcaccc ggttgtccgc ttcgcccttc gaattgtcgt
     2521 tcttgctgcc ggcgggcaaa caaaagccgt tctcatccgc gatgagcgtg aagcgaaacc
     2581 ttgttttgaa gtcgcccgag acttccgagc gcttgaatta aaaacaacac aagcgttatc
     2641 ggcctgcaat gaaaatcgcg attgtccgca tgccacatga tgaaattgcg ccacggcgcg
     2701 attgccggcc tgtgcataat ttagctgaca aggtcttgat gagtcgtggg gaattgcgag
     2761 ttttgttttt ctgattgaac gcggcgaaac agtctaaata attttaagac agtgcttacg
     2821 aaaaatgctt gtcacatatg cattttatgg tcctcgcttg cttatggccg ttaagccttt
     2881 catgcgccaa aaaaattcct gcgcgaaaat tcaagttagc cctcaatcaa aaaataaacg
     2941 ggcattggcc taagcacggt cggcgtcaga caaagtcgag caataccgcg gcgagcaaaa
     3001 attcttgacg cggcgatgac ggtttgacta cagcgtatgc gccgccgccg aattccccgc
     3061 cggatacaac cgttattggc aaaaatttgt agcaaaatgc ccgcgaagac ggcgggcttg
     3121 gacgaacgtg ctacttggcg agcttggcga tccgatgcga gagccgcgac accttgcggc
     3181 tggcattatt cttatggacg acgccgcgct gcgccgagcg catgatgacc ggctcggctt
     3241 ccttgaaggc cgccaaggcc gcattgcggt caccgctggc aatcgcttcc tcgatcttgc
     3301 ggaccgaatt gcgcagcacc gaacggcgcg ctttgttcac gatcgtccgg cgcgcgatct
     3361 tgcgcgtggc tttgcgggcg gatttggtat tggccatgag cctctcataa aacagaacta
     3421 gggcggcaaa acttaggcgg cgagaaccct cgccgccacc ggcgaggctt ataggagggc
     3481 cccgcctagg cgtcaacgcc gctattgagc ccgatcaggc gacccgtttg gcccggaccg
     3541 cgtcgttgcc ggcggtgacg gcggtacggt cggatgccga caggggcgcc agcggcggct
     3601 tgacccgcgc cagcgccggg tcgccatgga t
//
LOCUS       DASWZT010000321         7389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_70206, whole genome shotgun sequence.
ACCESSION   DASWZT010000321 DASWZT010000000
VERSION     DASWZT010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7389
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70206"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VGE92_08825"
     CDS             <1..415
                     /locus_tag="VGE92_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313968.1"
                     /translation="YYRAADLAKCQQWPVLREVLGSMAFDMPMILALFLLFAAKDGLQ
                     RRAADLDRLNCARRRAGKCALLEHIEVRAPIAVGYQCPASPAANANRRRGPRLHHVRG
                     HIARRGDKVFWRLPHLRGSARLGVVRSRTVQLSFH"
     gene            complement(416..1201)
                     /locus_tag="VGE92_08830"
     CDS             complement(416..1201)
                     /locus_tag="VGE92_08830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HEY1313969.1"
                     /translation="MSIRAIIVDDEELARRGIRALLQRAGDVEIVSECGSGDEAISAI
                     SAAEPEVVYLDIQMQGKTGFDVVAALPETKCPHVIFVTAFDRFAVRAFEIHALDYLLK
                     PVNEERFNVSLARARAAVAGVRDGSMIRRFWQMATELRAAPGQGLLPSMTDRIPVKTR
                     GRVMIINVAEIDWVEADGDYVSVHVGAKSWLLRETIAAAEARLALSGFVRIHRSTLVN
                     IYRVRELLPLSKGEFTVVLVDGTGLKLSRNYRFALERLLGKFL"
     gene            complement(1198..2373)
                     /locus_tag="VGE92_08835"
     CDS             complement(1198..2373)
                     /locus_tag="VGE92_08835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY1313970.1"
                     /translation="MNTDDSIALASGRTLSKATLFGVLHCGAWFGFGAFVLVFNIAEW
                     GLLPSMLDEFVWVAGGFTLTLGLRRVFRRARAAGWSYTSLGLLALTLSILVAPIWYAI
                     YLALLREGLACVALWPGLHAMFAHEANEAARLPWWIPIGYWNMFTSILLTWSSLYFGV
                     NAMLDLETERARSVRALKLADSARLRALQSQLNPHFLFNALNGIATLIREGDRTSAAD
                     TVDTLSDFLRLTLQKLDSPEIPVREELEFVEQYLRIQRLRFGSSFQVTVAADPETHDA
                     LVPTLILQPLVENAVRHGVLARAQGGTLSVSIRRRDAVLVITVEDNGPGIGDAGAHPY
                     GVGFKNSAERLAALYGDDAHMTVGARPYGRGFVVVVFLPFRQAPEMPANPARATLAV"
     gene            complement(2645..3124)
                     /locus_tag="VGE92_08840"
     CDS             complement(2645..3124)
                     /locus_tag="VGE92_08840"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY1313971.1"
                     /translation="MKNTALSRRTLLEASAWALAAGCGAPQIAAAHAEAGLSSRNGET
                     VRKYYTGWEKKDWPAFDMLLTDDFTFSSPLDARINKSAFKAGCWDTQIAYIGRFDLEH
                     VVGTGNDAFVMYVCHTSNDKTFRNVEYLRLKDGQVQAVECYFGGQNSFTSAVATGKS"
     gene            complement(3131..3778)
                     /locus_tag="VGE92_08845"
     CDS             complement(3131..3778)
                     /locus_tag="VGE92_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY1313972.1"
                     /translation="MDPQPSEPSRSGGARGKLLDAAISIIRKQGYAATSVDELCAAAG
                     VTKGAFFHHFPSKDSLAVAAANLWSESSAALFAAAPYHRFDDPLDRILGYLSFRKSML
                     RGDVAEFTCLAGTLVQETYRTHPDIRHACDASIGGHAAALESDFADAMKRYRLRAPYT
                     ARSLALHTQAVLQGAFILAKAKGNAAVVEASIEHLRRYIELLFRRPKKKASNAQE"
     gene            complement(3827..5686)
                     /locus_tag="VGE92_08850"
     CDS             complement(3827..5686)
                     /locus_tag="VGE92_08850"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY1313973.1"
                     /translation="MTSARNSPPVFPSDPEVLVDLARQDLASGQIAAAQEKCLRVLDA
                     HHHHPGALDVFGAILCSQGRYEEAVRVFNALTLIQPTVAAHWQNLGTVLRPTGRQAQS
                     LAAFDRALRLGPPTAGLLYNLGALQMDRLDYAAAHLALRDAVVLAPRDARIRWTFAQC
                     CYDMGQRQEAITAVEDWESLEGLSAEITVSIALLLVTSGAAHLAQPALQRLMAKPPAA
                     GRAAVGFAFLLERLHRLDEARTVMQQLELDEGSPSADLERLAMSALLADRAGRHEDAH
                     RLLTSALATQQDFVHQYNLLYPLAKTCDALGRYEEAYAAAAEAHRSQLAFLGRATGKT
                     SEEQSQLWALTAKGCDPEDIACWERAGPAMEVSPIFIVGFPRSGTTLLEQLLDAHPLL
                     QSMDEQPFLLQAVARVTDQGIGYPTELGRLSGSGLNDIRAHYWAAVRRRVPLAADRRL
                     VDKNPMNMVLLPMIRRLFPNAPVILAIRHPCDTVLSCFLQHFRSDLALLGRDLTAMAR
                     AYSRAFGFWYSQWPMLQPDTHELKYELLCADFASQVERLCAFLQLPWHEAMLAPGDHA
                     RAKGFISTPSYAQVLEPVSTRSVGRWQHYERHFGGEVLGLLIPWIERFGYSLG"
     gene            5811..>7389
                     /locus_tag="VGE92_08855"
     CDS             5811..>7389
                     /locus_tag="VGE92_08855"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1313974.1"
                     /translation="MNAVDQAKQAKLAVAVRAHRAGRLREAVADYEALLQRTPNDPDI
                     LQLLGVALAQSGNCADAVVFLARSVELNPDRPSVLLNLAQALGTLGRQEEALQACNRA
                     LALDASLAGGYRTRAAALTALGRREEALANAGQAVRLALQDAGAHADLGVALETVGRE
                     REALECFERAIALDPNLAAAHHNRGMLLARLGRHERALQSFDRALALQPHHAALHANR
                     GNALKQLGRLCEAEQSYSLALAIETHDPVILHSRALVRYLQRRYLEALRDYDTLAAQG
                     KENAPALVGRGATLVALRRYGEALAPLERAIQLLPEDADAHIQRGVALLNLERNAEAL
                     ASFDRALAIRPDAAEVLNNRGIALTATGRLRQALESFVRSAVQSGTTADNHTNIGIVL
                     KILGHHRQAIASFGRALALKPDDPSAKFALAFLHLTLGEFTVGWPLYEARFDVPALGN
                     PARHFIAPRWNGTESLAGKTLLVHAEQGLGDVIQFCRYLPLLAAQGVSVVFEVMPSLK
                     ALLRTLPAAIRLVGRGEP"
BASE COUNT         1400 a         2364 c         2270 g         1355 t
ORIGIN      
        1 ctactatcgt gccgcggacc tcgcgaaatg ccagcagtgg ccggtcctgc gtgaggtgct
       61 cggttcgatg gcgtttgata tgccgatgat actcgccctg ttcctgctat tcgcagcgaa
      121 agatggcttg caacgccgcg ctgcggacct agaccgcctg aattgcgccc ggcgccgcgc
      181 gggaaaatgc gcgctcctcg aacacatcga agtgcgggcg cccattgccg tgggctacca
      241 atgccccgcg tcgccggcgg ctaatgccaa tcggcgccgc gggccgcgct tgcaccatgt
      301 gcgcggccac attgcccgac gcggagacaa ggtgttttgg cgcctgccgc acttgcgcgg
      361 cagcgcgcgc ctgggtgtcg ttcgctcgcg aaccgtgcag ctgtcgtttc actgatcaca
      421 ggaatttccc caacaagcgc tcgagcgcga accggtaatt gcgactcagc ttcagtcccg
      481 tgccatcgac cagcacaacc gtaaactcgc ccttggacaa cggcagcagt tcgcgcacgc
      541 gatagatgtt gaccaacgta gagcgatgta tgcgcacgaa accggagagc gccaggcgcg
      601 cttccgccgc cgcgatagtc tcacgcagca gccagctctt ggcgccgacg tgcactgaca
      661 cgtaatcacc atcggcttcg acccagtcga tctcggcgac gtttatgatc atgacgcgac
      721 cgcgcgtctt cacaggaatt cgatccgtca tcgagggcaa caaaccctga cccggtgccg
      781 cgcgcaactc cgtcgccatt tgccagaagc gccggatcat ggagccgtca cgcacacccg
      841 caacggcggc gcgcgcgcgc gccagcgaca cgttgaagcg ctcttcgttg acgggtttca
      901 gcagatagtc gagcgcatgt atctcgaacg cccgcaccgc gaagcgatca aacgccgtga
      961 cgaaaatcac atgcgggcat ttggtttcag gcagcgccgc aaccacatcg aacccagttt
     1021 tcccctgcat ttgtatgtcg aggtacacga cttccggctc cgccgcactg attgcgctga
     1081 tcgcctcgtc accgctcccg cactccgata cgatctcgac atccccggcg cgctgcagca
     1141 gagcgcgaat gccgcgccgc gcaagttcct cgtcgtcgac aatgattgca cgaatgctca
     1201 taccgcgagc gtcgctcttg ccggattagc tggcatctcg ggtgcctgac gaaatggcag
     1261 gaacacaacc accacgaagc cgcgtccgta gggacgtgcg ccgaccgtca tgtgtgcgtc
     1321 atcgccgtac agtgcggcta accgctcggc cgagttcttg aatcccacgc cgtaagggtg
     1381 cgcgcctgcg tctccgattc ctggaccatt gtcctcgact gtaatcacga gcaccgcgtc
     1441 gcgccggcgt atcgatacgg atagcgtccc gccttgcgcc cgcgccagga ctccatgccg
     1501 caccgcgttc tccaccaatg gttgtagaat gagcgtcgga acaagtgcgt cgtgtgtttc
     1561 ggggtctgct gccacggtca cttgaaagga gctgccgaac cgtaggcgct ggatgcgcaa
     1621 gtattgctcg acaaattcca gctcctcgcg cacggggatt tccggcgagt cgagcttttg
     1681 cagagtcaat cttaggaagt cactcagcgt atcgacggtg tcggcggcgc tcgtacggtc
     1741 gccttcccga atcaacgtcg cgatgccgtt caaggcattg aataagaaat gcgggttcaa
     1801 ttgcgactgc aatgcacgca gccgcgcgct gtcggcaagt ttcagcgcgc ggacagagcg
     1861 cgcgcgctca gtctccaggt ccagcatggc attgacgccg aaatacaatg aagaccaggt
     1921 caacaggatg ctggtaaaca tgttccagta gccgattgga atccaccatg gtagccgcgc
     1981 cgcctcgttt gcctcgtgcg caaacatcgc gtgcaggccc ggccatagcg ccacgcaggc
     2041 aagaccttcg cgcagcagcg ccagataaat tgcgtaccaa atgggagcaa ccagaatgct
     2101 caatgtcagt gcgagcagcc ccagtgatgt gtacgaccag ccggccgcgc gggcgcgccg
     2161 gaagaccctg cgaaggccaa gagtcagcgt aaatccgccg gccacccaga caaactcgtc
     2221 gagcatcgag ggcaatagcc cccactcggc aatgttgaac accaggacga atgcgccgaa
     2281 gccaaaccag gccccgcagt gaagcacacc gaacagggtc gctttcgaaa gggtccggcc
     2341 agaggccaga gctatcgagt catcggtgtt catatccgtc ccgtcggcgc gagggtggca
     2401 tgggcgaaga tccgccatgt ctcgtaacgg atagcctagc gcacaactgc gggtgtcacg
     2461 catctcgcgt ggcgtactcg tccgcttcgt cgcaccgttg gtccgcttta ccgcaaaatt
     2521 tgcccaatcc acaacccagt tccacctcga cgcgcacccc ggcgccgcaa ctgccgccaa
     2581 cgcgagcgtg ggcgaacgca tcgccatgga ggaatatggc aaccgggcgg acggtgacac
     2641 ggtgttaact ttttccggtg gccaccgcag aggtgaagct gttttgccca ccgaagtagc
     2701 actcgacggc ctgcacctgt ccgtctttga gccgcaaata ctccacgttc cggaacgtct
     2761 tatcgttcga ggtgtgacag acgtacatca caaacgcatc gttgcccgtt ccaaccacgt
     2821 gctcgaggtc gaaccgcccg atatatgcaa tctgggtgtc ccagcatccc gccttgaaag
     2881 cgctcttgtt gatgcgggca tcgagtgggc ttgaaaatgt gaagtcatcc gtcagcagca
     2941 tgtcgaacgc gggccagtct tttttctccc agcccgtata gtatttgcgg acggtctcgc
     3001 cgtttcttga gctcagtccg gcctccgcgt gggcagcggc gatctgcggc gcgccacatc
     3061 ctgcggccag cgcccaggca ctcgcctcga gcagcgtgcg gcgcgatagt gcggtatttt
     3121 tcatgactca ttactcctgt gcattgctcg cctttttttt cggacgccgg aacaacaatt
     3181 caatgtagcg acgcagatgc tcgatgctcg cctccacaac cgcggcattg cccttggcct
     3241 tcgccaggat gaacgcaccc tgcagcacgg cctgcgtgtg cagtgccagg ctgcgggctg
     3301 tgtatggcgc acgcagccgg taacgcttca ttgcatcggc aaagtccgat tccaatgcgg
     3361 cggcatggcc gccgatgctt gcatcgcacg cgtgtcgaat gtcggggtgt gttcggtatg
     3421 tctcctgaac cagggtgccg gcgagacagg tgaactctgc aacgtcaccg cgcagcattg
     3481 acttgcggaa actgagatag ccgaggatgc gatcaagggg atcatcgaat cggtggtagg
     3541 gagcggcagc aaaaagcgcg gcgctcgact ccgaccagag attggcggct gcgaccgcga
     3601 gcgagtcctt gctcggaaag tggtgaaaaa acgcaccctt cgtcacgccg gccgcggcac
     3661 agagttcgtc gacggacgtt gcggcgtagc cctgcttgcg gatgatcgaa atggccgcat
     3721 cgagcagctt gcctcgggca ccgccggacc gtgacggctc actgggttgc ggatccatgc
     3781 agcgacagta ccaaccggtt ggtatggcgt caagcgcgaa tgctaactaa ccaagtgaat
     3841 agccgaagcg ctcgatccag gggataagca gacccaacac ttcgccaccg aagtgccgtt
     3901 cgtagtgctg ccagcggccc acggaccggg tgctgaccgg ctccaggacc tgtgcgtagc
     3961 tcggcgtgct gatgaaccct ttggcgcgtg cgtgatcccc gggagcgagc atcgcctcgt
     4021 gccaaggcag ctgcaaaaat gcgcacagcc gctcgacttg gctcgcgaag tccgcgcaga
     4081 gcagttcgta tttcagctcg tgagtgtcgg gttgcagcat gggccattgc gagtaccaga
     4141 atccgaaggc gcgcgagtaa gccctggcca tcgccgtcag gtcacggccc agcagtgcaa
     4201 gatccgagcg gaagtgctgc aggaaacaac tcagcacggt gtcgcaggga tggcgaatgg
     4261 cgaggatgac gggcgcgttc gggaacaagc ggcggatcat tggcagcagc accatgttca
     4321 tgggattttt gtccaccagc cgccgatccg cagccagcgg caccctcctc cgcacagcgg
     4381 cccaatagtg tgcgcggatg tcgttcaggc cgctgccgct caaccggccg agctcggtcg
     4441 gatacccgat accctgatcc gtgactcgag cgacagcttg caacaggaat ggctgctcat
     4501 ccatcgactg cagcagcgga tgtgcatcga gcagctgctc gagcagggtc gtacccgagc
     4561 gcggaaagcc cacgatgaat atgggactga cttccatggc aggtcccgcg cgctcccagc
     4621 acgcgatatc ctcggggtcg catcccttcg ccgtcagtgc ccaaagttgc gactgctctt
     4681 cggatgtctt gccggtcgcg cgcccgagaa acgcgagctg ggatcggtgc gcttctgcgg
     4741 cggcggcgta ggcctcctcg taacgtccga gcgcgtcgca agtcttcgca agtggataga
     4801 gtaaattgta ctgatgcacg aagtcctgct gcgtcgccag cgctgaggtc agcaaccgat
     4861 gcgcatcctc gtgcctgccg gccctgtcgg ccaacagcgc cgacattgcc aggcgttcca
     4921 aatcggcgct cggactcccc tcgtcgagct cgagctgttg catgacggtg cgcgcctcat
     4981 cgagccgatg taggcgctcg aggagaaatg caaatcccac tgctgcccgc ccggcagcag
     5041 gaggcttcgc catgagccgc tgcaatgccg gttgcgccag gtgcgctgcg cccgaggtga
     5101 ccaggagaag tgcgatggat accgtgatct cggcgctcaa gccttcgaga ctttcccagt
     5161 cttccacggc cgttatggct tcctggcgct ggcccatgtc gtaacagcac tgtgcaaagg
     5221 tccaacgaat ccgggcgtcg cgcggcgcaa gtaccacggc gtctcgcaag gcgaggtggg
     5281 ccgcagcgta atccaaacga tccatttgca atgcaccgag gttgtacagc aggccggcgg
     5341 tcggcggacc caggcgcaga gctcgatcga acgcggccag cgactgcgcc tgtcggccgg
     5401 tgggacgcag caccgtaccg agattttgcc aatgtgcggc gaccgtcggc tgtatcaggg
     5461 tgagcgcatt gaagacgcga accgcctcct cgtagcgccc ctgagagcag aggatcgctc
     5521 cgaaaacgtc cagcgcaccg ggatgatggt ggtgtgcatc cagcacccgc agacattttt
     5581 cctgcgcggc tgcaatctgg ccgctggcca gatcctgtcg cgcaagatcc accagcactt
     5641 cgggatcgga cgggaacacc ggcggtgaat tcctggctga tgtcaacgcg agttagcgca
     5701 gcggtcgagc aattatgcgg tgcccacgcg agtttcgcga acttgtcgcc gttgacgccc
     5761 cgcttgaggg gtggtgtatc ctttcgcgtc cctgaccttg agcaaatccc gtgaatgcag
     5821 tcgatcaagc caagcaggcg aagctcgccg tcgcggtgcg cgcgcatcgg gccggccgtc
     5881 tgcgtgaggc ggtggctgac tacgaggcgc tgctgcagcg tacgcccaac gatcccgata
     5941 ttctgcagct gctgggcgtc gcgctcgccc aatcaggaaa ttgtgcggat gcagtcgtat
     6001 ttctcgcaag gtcggtggaa ctcaatcccg atcgcccgag cgttctgctg aatctcgctc
     6061 aagcgctcgg cacccttggg cggcaagaag aggcgctcca ggcctgcaat cgcgcgcttg
     6121 cgctggatgc ttcgcttgcc ggcgggtatc gcacccgggc cgcggcactc acggcccttg
     6181 gacgtcgcga agaggcgctc gcgaacgcgg ggcaagcagt tcgtttggcc ctgcaggacg
     6241 cgggggcaca cgcggatctt ggtgtcgcac ttgaaacagt cggacgcgag cgggaggcct
     6301 tggagtgttt tgaacgggcc attgcgctcg acccgaacct ggcggccgca catcacaacc
     6361 gcggcatgtt gttggctcgc ttgggcagac acgagcgcgc gctgcagagt ttcgatcgcg
     6421 cgctcgcctt gcagccgcat catgcagcgc tccatgcgaa tcgcggcaac gccctcaagc
     6481 aactcggacg tctgtgcgaa gcggaacaga gctattcact ggcgcttgcg atcgagactc
     6541 acgaccccgt gatccttcac agccgtgcgc tcgtgcggta tctgcagcgc cgataccttg
     6601 aggcgctgcg ggactacgac acgcttgctg cgcaaggcaa ggaaaatgca cccgctctgg
     6661 tcggtcgcgg cgcgactctc gtcgcgctgc gccgctacgg cgaagcgctt gcaccgcttg
     6721 agcgcgcgat ccagctcctg cccgaagacg cggacgcgca cattcagcgc ggcgtcgcgc
     6781 tgctcaatct tgagcgcaac gccgaggcgc tcgcgagctt cgatcgggcc cttgccattc
     6841 ggccggacgc cgccgaagtg ttgaacaacc ggggtatcgc cctcacggcg acggggcggt
     6901 tgcgccaggc gctagagagt ttcgtccgct ctgccgtcca gagcggcacg actgccgaca
     6961 accacaccaa cataggcatt gtgctcaaga ttctcggcca tcatcgccaa gcgatcgcga
     7021 gcttcggtcg ggcgctggct ctcaaaccgg atgacccttc cgccaagttc gcgctggcgt
     7081 ttctgcatct gacgctcggc gaatttaccg tgggatggcc tctctatgag gcgcgcttcg
     7141 acgtgccggc gctcggtaat cctgcgcgcc acttcattgc accacgctgg aacgggacag
     7201 agtcgctcgc aggcaagacg ctactcgtac atgccgagca ggggttgggc gacgtgatcc
     7261 aattttgccg ctacctgccg ttgctcgctg cgcagggggt atccgtcgtg ttcgaggtga
     7321 tgccctcgct caaggcgctg ctgcgcactc tgccggcggc tatccggctc gtcggccgtg
     7381 gcgagccac
//
LOCUS       DASWZT010000322         2698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_7277, whole genome shotgun sequence.
ACCESSION   DASWZT010000322 DASWZT010000000
VERSION     DASWZT010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7277"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1779
                     /locus_tag="VGE92_08860"
     CDS             <1..1779
                     /locus_tag="VGE92_08860"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEY1313975.1"
                     /translation="ALLRAVSSRVGRPVSAATLDVQLISLHPRAVATRVTIGNPPWMP
                     QGSTAEIAKLTLVFDLPGLSHSFNIAKLVTEDTDLHLARDSAGRSNWQLTAPDQPAAA
                     IALIIRSLSMPGTHVELDDERRHLKFRGLVSAADIHDSGGAPALRLDGKGELNGRDVE
                     FDVTGDSLATARHDTPYHFSFVERSSGSRLSGNGSLPRPFDFNQVDAAFAATGADLKD
                     LYFLTGVTLVNTGSFALQGKFVRRGTQVTLSDLAVTSGQSDMQGTVWLDGSGERTQLR
                     ADLHSQSLRLADLGARAAGRDVESAANQSLLLSNAAFSPSAARRDDATVSLHARHAQV
                     GRVALQAVDAHMTLDHGVIVIAPLLADTLDGKLSARIQIDASKDDPPTSVDLKISDAQ
                     VGQLLRKGSAQAPLEGLLRARVLIKGHGSSVHQVASTANGTVSAVLSHGALRSSLAEL
                     TGGDLRGLGLLLAKSKQETGVRCGVASFRAQGGTLTAQSLVLDTDSFLISGEGAIHLD
                     SESLDLKLRGRPKKMRLVRVPSQVLLHGTLAHPSAGIGARNSATQTADAALGVLLTPL
                     ASILAFVDPGKPAEADCAALAAQPKT"
     gene            complement(1959..>2698)
                     /locus_tag="VGE92_08865"
     CDS             complement(1959..>2698)
                     /locus_tag="VGE92_08865"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: protein motif:HMM:TIGR01963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEY1313976.1"
                     /translation="GIGLGIARAFVAQGTVVAFSGFAPPDEIERISAELRGGGAEAVY
                     MPADARNPAELAALVRRCETQLGAVDILVNCAGIQHVSPIEEFPIAQWDAVIAVNLSS
                     AFHTMRAALPGMRRRNWGRIVNIASVHGLVASIDKSAYVAAKHGLIGLTKVVALETAG
                     SPITCNAICPGWVYTPLVQKQVDARAKELGIEVAHAQRLLLEEKQPSLQFVGIDEVAA
                     LVLFVASDAAREVRGAAWAIDGGWSAQ"
BASE COUNT          477 a          893 c          830 g          498 t
ORIGIN      
        1 gcgctgctgc gagccgtctc cagccgtgtg ggccgcccgg tcagcgcggc gacgctggat
       61 gttcagctaa tctcgctgca tccgcgagcg gtggcgacgc gggtcaccat cggcaatcca
      121 ccgtggatgc cgcaaggctc aaccgcggaa atcgccaagc tcactctggt gttcgaccta
      181 ccgggcttga gccattcctt caacatcgcc aaactcgtca cggaggatac agacttgcac
      241 ctcgcgcgcg attcggccgg ccgttccaac tggcaattga cggctccgga tcagccggct
      301 gcggcgattg cgctcatcat tcgcagcctc tccatgccgg gcacccacgt cgagcttgac
      361 gatgagcggc gacatttgaa gttccgcggc ctcgtctccg cggcagacat acatgattcc
      421 ggcggtgcgc ctgcgcttcg cctcgacggg aaaggggaat tgaacggccg ggatgtcgaa
      481 ttcgatgtta ccggcgactc gcttgccacc gcgcgtcacg acacgcccta tcacttttcc
      541 ttcgtggagc gatcgagcgg ttcgcgcctc agcggcaacg gatctttgcc gaggccattt
      601 gatttcaatc aggtcgacgc ggcattcgcc gcgaccggag cggacctcaa ggacctgtat
      661 tttttgaccg gcgtcacctt ggtgaatacc ggaagtttcg cgcttcaggg caagttcgtg
      721 cggcgcggaa cccaggtgac cttgagcgac ctcgccgtga cttcgggtca gagcgacatg
      781 cagggcaccg tgtggctcga cggctcgggc gagcgaacgc agctgcgcgc cgacctgcac
      841 tcgcagtcct tgcgtcttgc ggacctcggt gcacgggcgg ctggacgcga tgtcgagtcg
      901 gccgcgaatc agtctttgtt actgtcgaat gccgcgttta gtccgagcgc agcgcgccgc
      961 gacgacgcga cggtgagcct ccacgcccgg catgcgcaag tgggccgggt cgctttgcag
     1021 gcggtggacg cgcacatgac gctcgatcac ggcgtcatcg tgattgcgcc gctgttggcc
     1081 gacaccctcg atgggaaact cagcgcccgt atccaaattg atgcgtccaa ggacgatccg
     1141 cccacctccg tcgacttgaa aatctccgat gcacaggtgg gacagctcct tcgcaagggg
     1201 tcggcgcagg ccccgctgga gggtttgctg cgtgcgcggg tgctcatcaa gggacacggc
     1261 agttccgtgc atcaagtcgc atccactgcc aatggcacgg tgagcgcagt gctctcccac
     1321 ggagccctgc gttcgtcgct ggcggaactg accggcggcg atctgcgcgg gctcggcctg
     1381 ctgctcgcca agagtaaaca ggaaacgggc gtgcgctgtg gggtagcgag ttttcgggct
     1441 cagggcggca cgctcaccgc tcagagcttg gtcctcgaca ccgactcctt tctgatcagc
     1501 ggcgaaggtg ccattcacct cgattcggag tctctcgatc tgaagctacg cggtcgtccc
     1561 aagaagatgc ggctcgtgcg cgtgccctcg caggtgttgt tgcatggcac gcttgcgcat
     1621 ccctcggccg gcataggggc acgaaattcc gccacgcaaa ctgcggacgc cgcgctcggg
     1681 gtgttattga cgccgctcgc ctcgatactg gcctttgtcg atccgggcaa gcctgcggag
     1741 gccgattgtg ctgctctcgc cgcacagccg aaaacttagc tcatcgctgc gaagcggcgg
     1801 cttgcctgaa ccgtcgtaaa cagtggcgtt gcgaaaagcc agggtatgca gcgttctgcc
     1861 cgcgggatcc gccgcccaag ttaccagcgc gaacagcgca aggctcgcgc tgagcagcgt
     1921 tcggagtccg cacacgcggc gttcgacgct tggcacactc attgtgcgct ccatccaccg
     1981 tcgatggccc aagcggcgcc acgcacctcg cgcgctgcgt cgcttgccac aaatagcacc
     2041 agcgcggcca cctcatcgat gccgacgaat tgcagcgacg gttgcttctc ctcgagcaac
     2101 agccgctgcg cgtgcgcaac ctcgattccc agttccttgg cacgcgcatc gacttgcttc
     2161 tgcacgagcg gcgtgtacac ccagccggga caaatcgcgt tgcatgtgat cggcgatccg
     2221 gcagtctcga gcgccacgac cttggtcaat ccgatgagtc catgctttgc cgccacatac
     2281 gccgacttgt cgatcgaggc caccagtccg tgaacggacg cgatattcac aatccgcccc
     2341 caatttcggc ggcgcattcc cggcagcgcg gcgcgcatcg tatgaaacgc ggaagacaga
     2401 ttcacggcaa tgacggcgtc ccattgcgcg atcggaaact cctcgatggg cgatacatgt
     2461 tgaatgcccg cgcagttcac caagatatcg acggcaccga gttgcgtctc gcagcggcgc
     2521 accagagccg caagctcagc cgggttgcgc gcatcggcgg gcatatacac ggcctctgcg
     2581 cccccgcctc tgagctcggc gcttattcgc tcgatctcat cgggcggggc gaatccagaa
     2641 aacgccacga ccgtcccttg cgcaacaaac gcgcgagcga tgccgagccc aataccgc
//
LOCUS       DASWZT010000323        11377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_89512, whole genome shotgun sequence.
ACCESSION   DASWZT010000323 DASWZT010000000
VERSION     DASWZT010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11377
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89512"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1420)
                     /locus_tag="VGE92_08870"
     CDS             complement(<1..1420)
                     /locus_tag="VGE92_08870"
                     /inference="COORDINATES: protein motif:HMM:NF045284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313977.1"
                     /translation="MYQAGSEQHRKIVSAVARSSIRPPKTLRNVDWPRFLQAYYANVD
                     AEDLAAREPSELAGMALSHLLFARQRRRSALVRVFNPTLREYGYASPHTVIEMVNDDM
                     PFLVDSIGLALTQRALTLHFLTHPIFAVKRDAGGNLLALRERSETPQGAQPGHLKLRL
                     ESFQHIEVDRIVDPAVLAALALQIERSMRDVRVACADWLKMQQAAHEAAQELSSASAR
                     VDESNLNEAIALLSWMEDRHFTFLGYREYRLRGDKGRETLQPIAATGLGILRSGRKQP
                     ASTGRALSSDIRRQSRSRDIVLVTKANFESTVHRSGYLDYVGVKHFDGKGRLIGEKRF
                     LGLWTSTAYSSNPREIPVLRHKVAQVVQHFALAPDSHDGKALQHILESFPRDELFQAS
                     VAELNRIVTGIFGLQERPRVRLLLRRDAFRRFYSCLVYVPREKYNTQVRLRVERVVSE
                     AFDARGIESQVQIAESNLARIHI"
     gene            complement(1435..2601)
                     /locus_tag="VGE92_08875"
     CDS             complement(1435..2601)
                     /locus_tag="VGE92_08875"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEY1313978.1"
                     /translation="MSTFRLPDLGEGLAEAEILAWHVKVGDHVRVDQPMVSVETAKAV
                     VEVPAPFSGVVTALRGTPGDIVLTGAPLIEFDSGTVVGNMPATSAEEFVEPGGTGATR
                     SHGSNGRGRAVPAARALAKTLGVDLAAVEGGGRAGLITLDDVLRHANMPGAANGGAVG
                     SGSSPAARAIAAAAPQDGTVEPLRGMRRAMAQNMSQSRDQVPGSTVCDDADIHHWTQR
                     GDYMLRLMRAMSCAWRAEPALNAWYDPVTQSRFLVAHIDLAIAVDTPDGLIVPVVRNV
                     ESKSPQELRIDIAAQKDAAHRRSTAAQDLRDFTLMLSNFGTLAGRYGIPLVVPPAVAI
                     LGAGKVREDAVVVDGAVLAHRRMPLSLSFDHRCITGGEACRFLAAVIADLEKPN"
     gene            complement(2611..3573)
                     /locus_tag="VGE92_08880"
     CDS             complement(2611..3573)
                     /locus_tag="VGE92_08880"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY1313979.1"
                     /translation="MVEAIALAHGWEMQRDSNVVVIGQDVGANGGVFRATAGLQERFG
                     KDRVQDTPLAEATIAGMSVGMATHGLKPVAEIQFMGFIYPALDQIVNHMSRMRNRTRG
                     RLSCPVVIRTPHGGGIHAPEHHSESTEAMLCHIPGIRVVCPSSPARAYGLLLAAIRDP
                     DPVMFLEPVRLYRAMRQEVNDDGRALPLDVCFILREGSDVTLVTWGAMTVETLRAVAE
                     LDKRGISAEVIDLATLSPIDIETILESVAKTGRLVIVHEAVRNVGVGAEIAALVAEHG
                     LYDLQSPVRRVTGYDTVMPLFKLEYDYIPSVQRIIDAVELTFAD"
     gene            complement(3590..4672)
                     /gene="pdhA"
                     /locus_tag="VGE92_08885"
     CDS             complement(3590..4672)
                     /gene="pdhA"
                     /locus_tag="VGE92_08885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEY1313980.1"
                     /translation="MKTVAEFKIQYRQILTPGGELVAPLPEFAQDYAEVLRMYRAMTL
                     VRVFDTKSVNLQRTGQLGTYPSSLGHEAVHVGIGAAMRPEDVLAPVYREIGTQIWRGV
                     SMVEILTYWGGDERGNDFAVPRQDFAWCVPIATQTLHAAGAAMAFKVRNEPRCALAYI
                     GDGGTSEGAFYEALNLAGARALPVVFVVVNNGWAISVPVAAQTAAQTLAQKAVAAGIP
                     GVQVDGNDVFAVREVVSEALQNARRGAGPALVEALTYRLSDHTTADDASRYRSSQEVK
                     DAWQLEPLLRLREFLLRAGVWDSGKERAFLEECALEVDAAVAEYVAKPKPSTDAMFDH
                     LFASMPAHLQEQRLTARKYGAKPSGH"
     gene            complement(4699..5787)
                     /gene="zapE"
                     /locus_tag="VGE92_08890"
     CDS             complement(4699..5787)
                     /gene="zapE"
                     /locus_tag="VGE92_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019823840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEY1313981.1"
                     /translation="MASELKSVAARLRHEVAARRLEFDASQLDAALALDRLRSTLEAA
                     ESRWHWLKLRAARPVRGLYLWGGVGRGKTLLMDWFYDSLQTARRERTHFYRFMRQVHA
                     ELRSGSRRERPLDMVAQRLARQMDVICLDEFFVADIADAMLLAGLFDGLFRRGVALVA
                     TSNTPLEELYKDGLQRERFLPAIELIRRHVEVVHLDGGIDYRLRQLAQAPTYLDSSCA
                     QTLRQMQQFFAALAGDSALGPKTLSIEERGIAVLAVGGGMAWFDFAELCAGPRSQNDY
                     IELARLYHTIFISNIPEFTSADDDAARRFIAAIDEFYDRGVKLVVSAAAPPARLYHGE
                     RLVPEFERAASRLVEMQTQHYLAGQHRP"
     gene            complement(5804..7414)
                     /locus_tag="VGE92_08895"
     CDS             complement(5804..7414)
                     /locus_tag="VGE92_08895"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEY1313982.1"
                     /translation="MSESPRDVMEYEVAIVGGGPAGLSCAIRLKQLNPELSVCVLEKA
                     ASLGAHSLSGAVLEPGPLNELLPDWRSRAPAICVPATRDEFLFMTRRKRFRLPTPPQM
                     ANHGNFIISLGQLVPILGQEAEKLGVDVFAGFAAAAALIGDDGAVKGVRIGDMGVEKN
                     GEHGPNFALGPEIHAKVTMFCEGCRGSVSKQLIKRFNLDAGKSPQTYGLGFKELWQLP
                     PGRVQPGLIQHTLGWPLDSHTYGGSFLYHLDGDRVYVGFVVGLDYADPRLKPFEAFQQ
                     YKNHPDIKQLLQGGEIIAGGARTLIEGGYQSMPTLEMPGAMLVGDAGGTLNTPKIKGI
                     HQAIRSGMTAAAQFVEAGTSIGFDKRWRASTGGRELHKVRNIRPGFRHGLWVGLANAV
                     LETVTFGKLPWTLANHADDSALKKLSDYESPDRNWGERELPPRDRLAFVFFAATTHDE
                     NQPVHLHVADTAVCATRCITEFGNPCTNFCPANVYEMIADGNGGKRLQINSSNCVHCK
                     ACDIKEPYPGQITWVTPEGGSGPNYQSL"
     gene            complement(7629..8465)
                     /locus_tag="VGE92_08900"
     CDS             complement(7629..8465)
                     /locus_tag="VGE92_08900"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY1313983.1"
                     /translation="MHRRRRSDGDCRRTHLSASVLRSIEGGVGRITLNRPEKRNALDR
                     ATIGALTAELEQCAADPAVRVVLITGAGAAFCAGADLAEMQAQADANEADNLADARRL
                     ASMLAQLDSSPKPTIARVNGDGYGGALGLLGACDIVVAADTAKFAFTEVRLGIVPAVI
                     SPFVLAKIGESAARRYFLTAETLSAMTLKKLGLVHEVVAAPQLDDACASLTQSLLKGA
                     PLALAEAKSLIRDMAHRSALDRPAAALDMAARLARLRVAPEAREGFSAFFAKRKAGWR
                     TD"
     gene            complement(8417..9598)
                     /locus_tag="VGE92_08905"
     CDS             complement(8417..9598)
                     /locus_tag="VGE92_08905"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY1313984.1"
                     /translation="MNTDPVVILAARRTPVGAFQGVFASVSAPELGAAAIKAALSESG
                     LAASQVDEVIMGCVLSAGLGQAPARQAALGAGLGIEVPATTLNKMCGSAMRAVMSGCD
                     QIQAGSGKVIIAGGIESMTNAPYLLPKARAGYRMGNQEVLDHMFFDGLQSPWDGQLMG
                     CFAEATAEKYNFTRKAQDDFAAESVRRAVAAVNNGDFEAEIAPVTAKTRKGEVVVNKD
                     ETPFTLDIAKIPTLKPAFKKDGTVTAASSSGISDGAAALVLARESFAAAQSRKPLARI
                     LGYTSFAREPAWFTLAPVGAMQKLLKQLNWQARDVDLYEINEAFAAVPMAAIKDLGLD
                     PNKVNVNGGACALGHPIGATGARIMTTLLYALRARGKRRGIASLCIGGGEATAIAVEL
                     I"
     gene            complement(9646..10317)
                     /locus_tag="VGE92_08910"
     CDS             complement(9646..10317)
                     /locus_tag="VGE92_08910"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY1313985.1"
                     /translation="MYTGYCLGVFTVMGLTVLLINLFVPSLRRRRQVASAASRAFLRV
                     AGIAFTVEGLERLPQVPCVVVANHASYIDGLVAAAALPPDFAFVIKKEMVRVPLASLL
                     LRRLGSQFVERFNRHKGGVDARRVLKLAATGQSLVFFPEGTFNGVAQIGKFLGGAFTT
                     AARSDMPVVAMAIHGTRAVLPPDRLILHRLPIRVQILEVLPAGDAKLRSRELIAAALG
                     EPLAP"
     gene            10494..>11377
                     /gene="xerD"
                     /locus_tag="VGE92_08915"
     CDS             10494..>11377
                     /gene="xerD"
                     /locus_tag="VGE92_08915"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEY1313986.1"
                     /translation="MATALKRTVYTRTSGATVPAAREAADPALQRFLDAVWLERGLSA
                     NTLAAYRADLTALARWLEEHDSALLKATRIELLGFIAARVEGGSRPRSTARQLSSFRR
                     FFRFMMREGLIELDPTSQIAMPKIGRSLPKSLTEAEVDSLLEAPTVSDPLGHRDRTML
                     EVLYATGLRVSELVSLKHNQVNLTQGVMRVVGKGNRERLIPLGDEAVKWLQQFTQGPR
                     VEILLERQTDYLFPTRRGDRMTRQAFWHIIKRYSKKAGVEKELSPHTLRHAFATHLLN
                     HGADLRVVQMLLGHSDLST"
BASE COUNT         2094 a         3677 c         3553 g         2053 t
ORIGIN      
        1 cgatgtggat gcgggcgaga ttcgattcgg cgatctgcac ctgcgattcg atgccgcgcg
       61 cgtcgaacgc ctcgctcacc acccgctcga cgcgcagccg cacctgggtg ttgtattttt
      121 cgcgcggcac gtacaccagg caggaataaa aacggcgaaa cgcgtcgcgg cgcagcagca
      181 gccgcacgcg tgggcgctcc tgcaagccga aaatgccggt cacgatgcga ttcaattccg
      241 ctacgctcgc ctggaatagt tcgtcccggg ggaaggattc gaggatgtgc tgcagcgcct
      301 tcccgtcatg actatcggga gccagcgcga agtgttggac gacttgcgcc actttgtggc
      361 gcagcaccgg gatctcgcgc ggattgctgc tataggcggt cgaagtccac aaaccgagga
      421 atcgcttctc gccgatcaag cgccccttgc cgtcaaaatg cttgacgccg acgtagtcga
      481 gatagccgga acgatgaacg gtcgactcaa aatttgcctt ggtgaccagc acgatgtcac
      541 gggaccggct ctggcgccga atatcgctcg acagggcgcg gccggtggaa gcgggctgct
      601 tgcggccgct gcgcaatatc ccgaggccgg tggctgcgat cggttgcagg gtctcgcgcc
      661 ccttgtcgcc gcgcaggcgg tattcgcgat agccgagaaa cgtgaagtgg cgatcctcca
      721 tccaactgag gagcgcgatg gcttcgttca aattgctctc atcgacgcgc gcgctggcgg
      781 aactcaattc ctgcgccgcc tcgtgggcgg cctgttgcat cttgagccaa tcggcgcacg
      841 cgaccctgac gtcgcgcata ctgcgctcga tctgaagcgc cagcgcggcg agcacggccg
      901 ggtccacgat gcgatcgact tcgatgtgct gaaacgattc caagcgcagc ttgaggtggc
      961 cgggctgggc accttgcggc gtctcgctgc gctcgcgcag cgcgagtaaa ttgccgccag
     1021 cgtcgcgctt caccgcaaat atcggatgcg tcagaaagtg caaggtgagc gcgcgctgcg
     1081 tcagtgcaag accgatggaa tcgacgagga agggcatgtc gtcgttcacc atctcgatga
     1141 cggtgtgcgg cgatgcatag ccgtactcgc gcaaggtggg attgaagaca cgcaccagcg
     1201 ccgagcggcg cctttgacgc gcgaacagca aatgcgacag cgccatgccg gcgagctcgg
     1261 aaggctcgcg cgcggcgagg tcctcggcgt cgacattggc gtaatacgct tgcagaaatc
     1321 gcggccaatc cacgttgcgc agcgtcttcg gcgggcgaat cgacgagcgc gccacggcgg
     1381 agacgatctt acgatgttgt tccgagccag cctggtacat ggtaagtcct tgttttaatt
     1441 cggcttttcc aaatcggcaa tgacagcggc caagaatcgg cacgcctcgc cgcccgtgat
     1501 gcaacgatgg tcgaagctca aggacaacgg catccggcga tgagccagca ccgcgccgtc
     1561 gacgaccacg gcgtcctcac gcaccttgcc cgcacccaag atggcgacgg cgggcggcac
     1621 caccagcggg attccgtagc ggccggcgag agtcccgaaa ttcgacagca tcaaagtgaa
     1681 gtcgcgcaag tcctgggcag cggtgctgcg ccgatgtgcc gcgtcctttt gcgccgcgat
     1741 gtcgatccgc agttcttgcg gcgatttgct ttcgacattg cgcaccaccg gaacgatcag
     1801 cccgtcgggc gtgtcgaccg cgatcgcgag gtcgatgtgc gccaccagga atcgcgactg
     1861 cgtgacggga tcgtaccagg cgttcaaggc gggctccgcg cgccacgcac agctcatcgc
     1921 gcgcatcagc cgcagcatgt aatccccgcg ctgcgtccag tgatggatgt cggcgtcatc
     1981 gcataccgtg ctgccgggga cctggtctcg ggactgcgac atgttctgtg ccatcgcgcg
     2041 ccgcatgccg cgcagcggtt cgacggtgcc atcctgaggg gcggcggcgg cgatcgcgcg
     2101 tgcggccggc gaggagccgc tgccgaccgc gccgccgttc gccgcaccgg gcatgttggc
     2161 atgccgcagc acatcgtcga gcgtgatcaa tccggcacgg ccgccgcctt cgactgcggc
     2221 gagatcgaca cccaaggtct tcgccagcgc gcgcgccgcc ggcaccgcgc ggccgcggcc
     2281 attgctgccg tggctgcggg tcgcgccggt gccgcccggc tcgacgaatt cctccgcgct
     2341 ggtcgccggc atgttgccga cgacggttcc ggaatcgaac tcgatgagcg gggcgcccgt
     2401 gagaacgatg tcgccgggcg tcccgcgcaa ggcggtcacc acgccactga aaggcgccgg
     2461 cacttcgacc accgccttcg ccgtttcgac cgagaccatg ggttgatcga cgcgtacatg
     2521 atcgcccacc ttgacgtgcc atgcgaggat ctccgcctcg gccaaaccct cgcccaaatc
     2581 cggaagcctg aatgtactca tgagcgacct ctagtcggcg aaagtcagtt ctaccgcatc
     2641 gatgattcgc tgcacgctcg gtatgtagtc gtactcgagc ttgaacagcg gcatcacggt
     2701 gtcatatccg gtcacgcgcc gcaccggcga ctgcagatcg tacagcccat gctccgcaac
     2761 cagcgccgcg atctcggctc cgacgccgac attgcgtacc gcctcgtgca cgatcaccag
     2821 ccggccggtt ttcgccaccg attcgagaat ggtttcgatg tcgatcggac tcaaggttgc
     2881 aagatcgatc acctcggcgc tgatcccgcg cttgtcgagc tccgccaccg cgcgcaacgt
     2941 ctccacggtc atggcacccc aagtgacgag agtcacgtcg ctaccttcgc gcaagatgaa
     3001 gcacacgtcc aacggcaagg cgcggccgtc atcgttcact tcctggcgca tcgcgcgata
     3061 tagacgcacc ggctccaaga acatcaccgg gtccggatcg cgaatggccg ccagcagcag
     3121 gccgtaggcg cgcgccggcg atgaggggca aacgacgcga attcccggaa tgtggcacag
     3181 catcgcttcg gtgctttccg aatgatgttc gggcgcatga atccctccgc cgtggggtgt
     3241 tctgatcacg accgggcaac tcaagcgtcc acgggtgcgg ttacgcatcc gcgacatgtg
     3301 atttacgatc tgatcgagcg ccggatagat gaatcccatg aactgaattt ccgcgacggg
     3361 tttcaaaccg tgcgtcgcca tgcccaccga catgcccgcg atggtggcct cggcgagcgg
     3421 cgtatcctgg actcgatcct tgccgaagcg ctcctgcaat ccggcggtgg cgcgaaacac
     3481 gccgccgttg gcgcccacgt cctgaccgat gacgaccaca ttggagtcac gctgcatttc
     3541 ccagccatgt gccagcgcaa tggcctctac catggttatt ttcgccaatt caatgcccgc
     3601 tgggtttcgc gccgtatttg cgggccgtga gacgctgttc ttgcaaatgt gccggcatgg
     3661 atgcgaacag atgatcgaac atcgcatccg tagacggctt tggtttggcc acgtattccg
     3721 ccaccgcggc atcgacctcg agcgcgcatt cctcgagaaa ggcccgttcc ttgccgctat
     3781 cccaaacgcc tgctctcagc agaaactccc gcagccgcag cagcggttcg agctgccacg
     3841 cgtctttgac ttcctgactc gaacgatagc ggctggcatc atccgcggtg gtgtggtcgc
     3901 ttaggcggta ggtcaacgcc tcgaccagtg cgggacccgc accgcggcgc gcgttctgca
     3961 gcgcctcgct gaccacctcg cggacggcga atacatcgtt gccgtcgact tgtacgcccg
     4021 gaatgcccgc ggcgaccgcc ttctgtgcca gcgtttgcgc cgccgtctgt gccgcgaccg
     4081 gaacggagat tgcccagccg ttgttgacga cgacgaatac caccggcagc gcgcgcgcgc
     4141 cggcgagatt cagcgcctcg tagaaagcgc cttcggaagt gccgccgtcg ccgatgtacg
     4201 cgagcgcgca gcgcggctcg ttacgcactt tgaacgccat ggccgcgccg gccgcatgca
     4261 aagtctgcgt ggcaatgggc acgcaccacg cgaagtcctg gcgtggcacc gcaaaatcgt
     4321 tgccgcgttc gtcgccgccc cagtacgtca agatttccac catgctcacg ccgcgccata
     4381 tctgcgtgcc gatttcgcga tacaccggcg cgagtacatc ctcgggccgc atcgcggcgc
     4441 cgatgccgac atgtacggct tcgtggccaa gtgaggaggg ataggtaccg agttgtccgg
     4501 tgcgctgcag gttcaccgac ttcgtgtcga aaacccggac cagtgtcatg gctcgataca
     4561 tgcgcaggac ttcggcatag tcctgcgcaa actcgggcag cggcgccacc agttcgccgc
     4621 cgggggtcaa aatctgccgg tactgaatct tgaactcggc gaccgtcttc atcgtcgaat
     4681 tatgccaggg aacctcagtc aagggcgatg ttgaccggcg aggtagtgtt gtgtctgcat
     4741 ctcgacgagg cggctcgcgg cgcgttcgaa ctcaggcaca aggcgctcac cgtgatacaa
     4801 ccgcgccggc ggcgccgccg cggacaccac gagcttgacg ccgcgatcat agaattcgtc
     4861 gatggctgcg atgaaacggc gcgccgcgtc gtcgtccgcc gaggtgaatt cggggatgtt
     4921 cgaaatgaaa atcgtgtgat acaagcgcgc aagttcgatg tagtcgttct ggctgcgcgg
     4981 cccggcgcac agttccgcga agtcgaacca agccatgccg cccccgaccg ccagcaccgc
     5041 gatgccgcgc tcctcgatcg acaaggtctt tggacccaac gcggaatcgc cggcgagagc
     5101 ggcaaagaac tgttgcattt gccgcagcgt ttgcgcgcag ctcgagtcca ggtaagtcgg
     5161 cgcctgcgcg agctgacgca atcgatagtc gatgccgccg tccaagtgca ccacctcgac
     5221 gtgcctgcgg atcagctcga tggcgggcag gaaccgctca cgctgcagcc catctttgta
     5281 gagctcttcg agcggcgtgt tggaggtcgc gaccaacgcg accccgcggc ggaagagtcc
     5341 gtcgaacagg cccgcaagca gcatcgcgtc ggcgatatcg gcgacgaaga attcgtccag
     5401 acagattacg tccatctgcc gcgcgaggcg ctgcgcaacc atatccaacg ggcgttcgcg
     5461 gcgcgacccg ctgcgcagtt ccgcatgcac ttggcgcatg aagcgataga agtgggtgcg
     5521 ctcgcgccgc gcggtttgca gcgagtcgta gaaccagtcc atcaagagcg ttttgccgcg
     5581 gcccactccg ccccacaggt acaggccgcg caccggccgc gccgcgcgca gcttcagcca
     5641 gtgccaacgc gactcggctg cctcgagagt cgagcgcagc cgatcgaggg caagcgcggc
     5701 atcgagttgg cttgcgtcga attcgagacg ccgcgccgcg acctcgtgac gcaggcgcgc
     5761 tgcaaccgac ttaagttcgc tggccactgc cgaggcggca atcctagagg ctctggtagt
     5821 tgggacccga gccgccctcc ggcgtcaccc aggtgatctg cccaggatag ggttccttga
     5881 tgtcgcaggc cttgcagtgc acgcagttcg aggaattgat ctgcaatctc ttgccgccgt
     5941 tgccatcggc gatcatctcg taaacattcg ccggacagaa attcgtgcac gggttgccga
     6001 attccgtgat gcaacgcgtc gcgcaaacgg cggtatccgc cacatgcaga tgcaccggtt
     6061 gattctcatc gtgcgtggtt gcggcaaaga agacgaacgc cagccggtcg cgcggcggca
     6121 attctcgttc gccccagttg cggtccggac tttcatagtc actcaatttc ttgagcgcgg
     6181 aatcgtccgc gtgattggcg agcgtccagg gcaatttccc gaacgtaacg gtttccaaga
     6241 cggcattggc gaggccaacc cacaaaccat gacgaaaccc gggacggatg ttgcgcacct
     6301 tgtgcagctc gcgacccccc gtcgaggcgc gccagcgctt gtcgaagcca atgctcgtgc
     6361 cggcttcgac gaactgcgcg gcggcggtca tgcccgaacg aatcgcctgg tggatgcctt
     6421 tgatcttcgg tgtgttgagg gtgccgccgg catcgcccac cagcatcgcc cccggcatct
     6481 cgagcgtcgg catggattga tagccgcctt cgatcagcgt gcgggcgccg ccggcgatga
     6541 tctcgccgcc ttgcagtaat tgcttgatgt cggggtgatt cttgtactgc tgaaacgctt
     6601 cgaacggttt gaggcgcgga tccgcataat ctagtccgac cacgaatccg acgtatacac
     6661 ggtcgccgtc caggtggtac agaaagctgc cgccataggt atggctgtcg agcggccatc
     6721 ctaaggtgtg ttgaatcaat ccgggctgca cccgtcccgg tggcagctgc cacagctctt
     6781 tgaaccccag cccgtaagtc tgcggcgact tcccggcatc gaggttgaat cgcttgatca
     6841 actgtttcga gacgctgccg cggcaaccct cgcagaacat ggtgaccttg gcgtgaatct
     6901 cgggtcccaa cgcgaaatta ggtccgtgct cgccgttctt ttcgacgccc atgtcgccga
     6961 tgcgcacgcc cttgaccgcg ccgtcgtcgc cgatcaacgc cgccgccgcc gcaaacccgg
     7021 caaacacatc gacaccgagc ttttcggctt cttgaccgag aatcggcacc aattggccaa
     7081 gcgagatgat gaaattgccg tgattcgcca tctgcggcgg agtcggcagc cgaaagcgtt
     7141 tgcgccgcgt catgaacaga aattcgtcgc gcgtcgcggg gacacagatt gccggggcgc
     7201 ggctgcgcca gtcgggcagc agctcattca gcgggccggg ctcgagcacg gcgcctgaaa
     7261 gcgaatgcgc gccgaggctg gcggcttttt cgagcacgca gacgctcaat tcggggttca
     7321 gctgcttgag ccggattgcg caggagaggc cggcgggacc gccaccgacg atcgcgacct
     7381 cgtattccat gacatctcgg ggactttcgc tcatcaattc cacctgaaat cgagggtggc
     7441 tagtagatca caaaccggac atagcgggga ggcgtccatc tggtggacat ccattgaggg
     7501 gtttttcgta gctacgtcgc cttaatagat agcggggccc atgttgacag gccaaggatg
     7561 ccggctaccg atgaggccaa cggatttggg catgggatgg cagggacgcc ccccgctttt
     7621 tcttgacgct agtcggttcg ccatcccgcc ttgcgcttgg cgaagaacgc gctgaagcct
     7681 tcgcgcgctt ccggcgcgac ccgcagtcgc gcaagtctcg ctgccatatc gagcgccgct
     7741 gcgggccgat cgagagcgga gcgatgtgcc atatcgcgta tcagtgactt ggcctcggcg
     7801 agcgccaacg gtgccccctt gagcagggac tgcgtgagcg atgcgcaggc gtcgtcgagt
     7861 tgcggggcgg ccacgacttc atgcaccagc ccgagctttt tgagcgtcat ggccgacagc
     7921 gtctcggcgg tcaagaaata tcgtcgtgcc gcgctctccc cgatcttggc gagcacgaac
     7981 ggcgagatga cggccggaac gataccgagt cgcacttcgg tgaaagcgaa tttggcggtg
     8041 tccgccgcta cgacgatatc gcaggcgccg agcagcccga gggccccgcc ataaccgtcg
     8101 ccattgactc gggctatcgt tggcttaggg ctggaatcca attgcgccag catgctcgcc
     8161 aggcgccggg catctgccaa attgtccgct tcgttcgcgt cggcttgagc ttgcatctcg
     8221 gccagatcgg cgcccgcaca aaaggcggca cccgcccccg tgatcagcac cacccgcacc
     8281 gcggggtctg cggcgcattg ctcgagctcc gcggtcaatg cgccgatcgt ggcccggtcc
     8341 aacgcgttgc gtttctcggg gcgattcagt gtgatccgcc cgacgccgcc ctcgatcgac
     8401 ctgagaaccg aagcgctcag atgagttcga cggcaatcgc cgtcgcttcg ccgccgccga
     8461 tgcacagact cgcgatgccg cgacgcttgc cgcgcgcacg cagggcatac agcagggttg
     8521 tcatgatgcg cgctccggtc gcgccgatcg gatgccccaa cgcgcaggcg ccgccgttca
     8581 catttacttt gttgggatcg aggccgaggt ccttgatggc cgccatggga actgcggcga
     8641 aagcctcgtt aatctcgtac agatcgacat cccgcgcttg ccaattgagc tgcttcaata
     8701 acttttgcat ggcacccacc ggagccagcg tgaaccaggc cggctcgcgc gcgaaactcg
     8761 tgtagcccaa aatccgcgcc agcggtttgc ggctctgcgc tgccgcaaag gactcgcgcg
     8821 ccagaacgag tgccgcggcg ccatcggaaa tccccgagga actggcggcc gtcaccgtgc
     8881 cgtccttttt gaacgcaggc ttgagagtcg gaattttggc gatatcgagc gtgaaggggg
     8941 tctcgtcctt gttgaccacg acctcgccct tacgcgtctt cgccgtgacc ggggcgatct
     9001 cggcctcgaa atcgccatta ttgacggcgg ccacggcgcg ccgcaccgat tcggcagcga
     9061 agtcgtcctg ggctttgcgc gtgaagttgt atttctcggc ggtcgcttcg gcaaaacaac
     9121 ccatgagctg cccatcccag gggctttgca agccgtcgaa gaacatatgg tcgagcacct
     9181 cctgatttcc catgcggtag ccggcacggg ccttgggcaa gaggtagggg gcgttggtca
     9241 tcgattcgat tccgcctgcg atgatgactt tgccggagcc ggcctggatc tggtcgcagc
     9301 cggacatcac cgcgcgcatc gcggagccac acatcttgtt gagcgtggtc gccggaacct
     9361 cgatgccgag gcccgcaccc agggccgcct gccgtgcggg tgcctggcca agtcctgccg
     9421 acaggacaca gcccatgatg acctcgtcga cttgggacgc ggcgaggccg gactcgctca
     9481 gcgccgcctt gatcgccgcc gcaccgagct cgggggcact gaccgaggcg aagacacctt
     9541 gaaatgcgcc gaccggcgtg cggcgggcgg ccagtatgac aacgggatca gtgttcatgg
     9601 cggcatttcc gtgacatggg gagcgggtcc ctagtatagc tgcgcttacg gcgccagcgg
     9661 ctctccgaga gcagccgcga tcagctcgcg gctgcgcagc ttggcatcgc cggcgggcag
     9721 cacctcgagt atctgcacgc gaatgggcag acggtgcaga atcaggcggt ccggtggcag
     9781 gactgcgcgc gtcccgtgaa tcgccatcgc caccaccggc atgtccgagc gtgccgccgt
     9841 ggtgaacgcg ccgccgagaa acttgccgat ttgtgcaacc ccattgaacg tcccctctgg
     9901 aaagaacacc aaggactgac cggtggcggc gagtttgagg acgcgacgtg catcgacacc
     9961 gcccttatgt cgattgaagc gttcgacgaa ctgcgagcct agacgtcgta gcaacagact
    10021 cgcgagcggc acgcgcacca tctctttttt gatcacgaag gcaaaatcgg gcggcagtgc
    10081 cgcggccgcg accaggccgt cgatataact cgcatgattc gccacgacca cgcaggggac
    10141 ctgcggcagg cgctccaagc cctcaaccgt gaaggcgatg ccggcgaccc gtaaaaaggc
    10201 gcgcgaagcc gcgctcgcta cctgccgccg ccggcgcaaa ctcggcacga acaaattgat
    10261 caacagcacg gtcaaaccca tgaccgtgaa gaccccgagg cagtagcccg tgtacaccga
    10321 ctgtgaggct ctgcacagag ttgtccgctc gattactgtg atctggttct ccttatgtta
    10381 atacttaacc gtactgtgat tctggtcacg ccgacctgaa tgcacccctc gcatactgat
    10441 cttaagacgc atttacccag cacgaattgg tgcgattact cacgcggaag attatggcga
    10501 cggctctcaa acgcacggtt tatacacgca cctcgggcgc aacggtaccc gccgctcgcg
    10561 aagcggcgga tccggctctg cagcgctttc tcgacgccgt gtggctggag cgcgggctgt
    10621 cggcgaatac tctcgcggcc taccgcgccg atttgaccgc gctggcgcgc tggttggaag
    10681 agcacgacag cgcattgctc aaagccacgc gcattgaatt gctcggcttc atcgccgccc
    10741 gcgtcgaagg cggatcaaga ccccgatcga cggcgcggca gctgtcgagt tttagacgct
    10801 tcttccgctt catgatgcgc gaggggttga tcgaactcga tccgacctcg caaatcgcca
    10861 tgccgaagat cggccgctcg ttgcccaagt cgctgaccga agccgaggtc gactcgttgc
    10921 tcgaggcgcc gacggtatcg gacccgctcg ggcatcgcga ccgcaccatg ctcgaagtcc
    10981 tatacgcgac cggactgcgc gtgtcggaac tcgtgagtct gaagcacaac caggtcaacc
    11041 tcacgcaagg cgtgatgcgg gtggtcggca agggcaatcg tgaacgcttg atcccgctcg
    11101 gcgacgaggc ggtgaagtgg ctgcagcaat tcactcaggg cccgcgcgtc gagatcctgc
    11161 tggaacggca gaccgattat ctgtttccga cgcgccgcgg cgatcgcatg actcgccagg
    11221 cgttttggca cattatcaag cgctattcga aaaaggcggg cgtggagaag gagctctcgc
    11281 cgcataccct gcggcacgcc ttcgcgacgc atctgttaaa tcacggcgcg gacctgcgcg
    11341 tggtacagat gctgctcggg cacagcgacc tgtcgac
//
LOCUS       DASWZT010000324         8342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_50789, whole genome shotgun sequence.
ACCESSION   DASWZT010000324 DASWZT010000000
VERSION     DASWZT010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8342
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50789"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="VGE92_08920"
     CDS             <1..898
                     /locus_tag="VGE92_08920"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1313987.1"
                     /translation="SRAEQQVRSGKVQVAALLPQNFGAEATRALRGADTKPRVELLID
                     PSQAMSGRVVEGLFAQHAMHEISKAAFSGDAASGLQGGIKLPYVIAIKVTAPAKIPYN
                     GYAHSFAGMTTQFILFAGIDAGILLLLTRERGIWQRLRSAPLSKTEFLLARTLATTLI
                     SLFQFAAIYCAAVGIFGVRISGSVLGFAALAVALCVLNATFGLMLSSIGRSAGATRGI
                     AMVVTLLLVMVGGAWVPSFVFPSWLQRASLAAPTRWAVDGLDAMTWRGLGLDAAVAPL
                     LVLAASAVMCLAIAIWQFRWED"
     gene            927..2195
                     /locus_tag="VGE92_08925"
     CDS             927..2195
                     /locus_tag="VGE92_08925"
                     /inference="COORDINATES: protein motif:HMM:NF021508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HEY1313988.1"
                     /translation="MSAAIPPGKRLALFFDGTWNEPPDHTNVRRLRLMLGERGADGLE
                     QRAFYDPGIGTRWYDRLSGGLIGAGLSANIRNGYRWLVENYDEGDEIYLFGFSRGAFA
                     ARSLAGIIARYGLLRADAPISFSQLYERYQKGDAVATIYELIRAKHQSVTLGFEDQAL
                     LRHSYYRRNMIKMVGVWDTVGSIGLPFGTFPGLSRRMLKFHNTNLSVTIEHSYQALAM
                     DEHRAPYWAVLWTHFRPIAPDVKPVARDDERWIEQRWFPGAHANVGGGYRLDPLPNRP
                     LRWLQAKAAACGLDFRRLADPADDSLAVMPRDSYREFLHGFWRIATFGRRYVRWVFSD
                     PVRKLSAAGTNHAVLGWLQTVNERIDESVWQLCRDHPTYRPLGLLEWARRTGQDLEAL
                     IANPQHWMAFSAAIDRPGIEMRDPPPVDEE"
     gene            2185..2904
                     /locus_tag="VGE92_08930"
     CDS             2185..2904
                     /locus_tag="VGE92_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313989.1"
                     /translation="MRNNRPAWRLAARAAGGCARACGVLLLCASSLCRAASPPQPSAW
                     TESFVSRVEALALLETLNSDLLSHDSATATLERWCAAHRLATPAHMVAVRVPNVDKPP
                     SPALRSLLKVAPQTAVRYRRVRLECGALVLSEADNWYVPDRLTPAMNALLDSTETPFG
                     VVVRPLNFQRHTLSSTLLWMPLPAGWEMDAHSQRQLGAEPIRSQILEHRALLTLPDGT
                     PFSTVIETYTNNVLAFAAVQP"
     gene            2990..3982
                     /locus_tag="VGE92_08935"
     CDS             2990..3982
                     /locus_tag="VGE92_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1313990.1"
                     /translation="MRLLDKVARSHRPFVLALDRMPGTALEVTGPSCYAQQVAQCPLR
                     FVLGDDLTRACAELAFADGTRLSGCLDLLRIPARRLWIEWNDEVHQRVICETQSDAAY
                     PVACSGRRVGVLLDGAEDGWRAVMRTFWADAADEASEATLSPLETHLDLRGGFTLPGG
                     IACDSGRANAATRCLLEHARFRFDDAWAAYYRAAAASAVTQQDVVHRSVAAVAADAPL
                     ILAFFLLLSAQGATRSVQVSRAAINRKRLAHGRPPLLDHIEVRASLDAVAAAEPAGLE
                     PSGRQSPRLHHVRGHLVRREDRVFWRVPHLRGSALRGMVRSRTVCLSFAQQRLN"
     gene            complement(3979..4911)
                     /locus_tag="VGE92_08940"
     CDS             complement(3979..4911)
                     /locus_tag="VGE92_08940"
                     /inference="COORDINATES: protein motif:HMM:NF004793.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEY1313991.1"
                     /translation="MIHIDFEAVHRLASVGSLVEPPRRAFGSPAISPERAHYDLPSPK
                     TPRSLLLMPSWQPAGGIGVKIVTVFPDNDARGLPSVNAAYVLLSGQTGQPRALLDGRA
                     LTLLRTAGVSALAADLLAPTDPGRLLMVGTGALSRYLIEGHLAVRQYESVQIWGRDRT
                     KAATVARDLASRGWPITVADELEIAVRSATVVSCATLAQTPLIEGGWLRRGAHLDLVG
                     SFRPDMREADDACMTGALVVVDTRSALEESGDLAEPIARGVLAADDIFTLNTLLATSC
                     AAPRGSRTVFKSVGVAHADLAVAEHLLERSGLGG"
     gene            complement(4920..6107)
                     /locus_tag="VGE92_08945"
     CDS             complement(4920..6107)
                     /locus_tag="VGE92_08945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY1313992.1"
                     /translation="MSTEVAVIGGGVMGSALAYWLTRLEPKLAVTVIERDPSYQHASS
                     ALSAASIRQQFSTPVNIRISQASIAFLRNAALELGVDGEQPNISLREAGYLYLATAAN
                     ARELRRSHALQRSCGADIALLERGELAARFGWLNTRDVSVGTLGLSGEGWFDGYALLM
                     AFARKARAQGAHYLNAEVCSLGIADRRVTEVIMADGARLGCRHVVNAAGPWARRIAAL
                     AGLELPVFAQRRTVYVISCPLKLTPFPLLIDTSGFWIRPEGTKFIAGLSAASYRAAPA
                     DALNDEPLQPDYDAFETRLWPALAHRIAAFEQARLERAWAGYFEMNAFDQNGILGPHP
                     ALENYWFMNGFSGHGMQQAPIVGRGVAELILCGRFDSLDLSDLLYARIAGQRPLYEAN
                     VIG"
     gene            6293..>8342
                     /locus_tag="VGE92_08950"
     CDS             6293..>8342
                     /locus_tag="VGE92_08950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY1313993.1"
                     /translation="MQSALVKSQSAAAPAAGRKSARSPKNAKLSAYLVTRDDTLWPQI
                     GAHIGQQLILKQSDSVDELLATTPREHPAIVLWDARNQPEAAIVLSRLQQHSACFAVV
                     ALDDAGGSSGWQVVVNVSIPVMPHELTAALQIATEEVRARVALLGEGGAEAHAAAPSA
                     SEPTVEDASHISVSVTPSAVGAAEPKRATHRRSRSRRFPWTPVLIVIGLGIVCIGIYV
                     VTQPQDTPLKPTPPAMLPAAVPAAKAPAALPKLPAADDKLDLLIESAQKAMLDRHFIE
                     PAEGSALSLYRSVLLLDPSNGEANQGLLRLAEVLFTRVRSALDERKFDVALQALETAR
                     SINPSDPRLAAFDQRIAALRAELGPAQIQAAINAQNFDRASQLIDEAARAKSLSAAKL
                     AQLRDDLQLEREESDVANFLKLIDSRLQQDKVIEPREDSAAYYIGRARDAGASAAALQ
                     AQSQEVDKRLALTVRAAIEQRRFADAERMLTYLRADGVAAATLAPLQRDLLAARTQAA
                     GHDQPQALDLAVSRLAQGKVTEPDNDSALFYVNQLRAADPNNAGLPRISGAVQAQILE
                     QARASLDATQVGAAESLLKLAAGLGSSSVLDTLNQRLAQAKSSAGAALPEVTESSLTR
                     INAIETSYPAMARRKNIEGWVELSYQVTAQGSVAQIKVVNSSPAGIFDDAAAKALARV
                     R"
BASE COUNT         1454 a         2728 c         2730 g         1430 t
ORIGIN      
        1 ctcgcgggcc gagcagcaag tgcgcagcgg caaagtgcaa gtcgccgccc tgctgccgca
       61 aaatttcggc gccgaggcaa cgcgcgcgct caggggcgca gacacgaaac cacgggtcga
      121 gcttctcatc gatcccagcc aggcgatgag cggccgcgtc gtcgagggtc tgttcgcgca
      181 gcatgccatg cacgaaatct ccaaggcggc attcagtggc gacgcggcgt cggggttgca
      241 gggcggcatt aagcttccct acgtgatcgc catcaaggtc accgcgcccg cgaaaattcc
      301 ctacaatggg tatgcgcatt cgttcgcagg aatgaccacc cagttcattt tgttcgccgg
      361 catcgacgcc gggatactgc tgctgctcac ccgtgagcgc ggcatctggc agagattgcg
      421 ctcggcgccg ctcagcaaga cggaatttct gctggcgcga acgcttgcga cgacgctgat
      481 cagcctgttt cagttcgcag cgatctattg cgccgccgtc ggcatctttg gcgtgcgaat
      541 ctccggcagc gtgctcggtt ttgcggccct cgccgttgcg ctgtgcgtgt tgaacgcgac
      601 cttcggcctg atgctgtcct cgatcgggcg cagcgccggg gcgacccgcg gcattgccat
      661 ggtggtcacg ttgctgttgg tgatggtcgg cggcgcgtgg gtgccgtcct tcgtattccc
      721 gtcctggctg caacgcgcct cgctggcggc accgacgcgc tgggcggtcg acggcttgga
      781 cgccatgacc tggcgaggtc tcgggctcga tgcggccgtg gcgccgctgc tggtgctcgc
      841 cgcctccgcc gtcatgtgtc tcgcgatcgc catctggcag tttcgttggg aagactaggc
      901 ggagagagtc gcgggatgcg aaacacgtga gtgcagcgat tccaccgggc aagcgtctgg
      961 cgctattttt cgacggcacg tggaacgagc cgccggatca caccaacgtg cgccgcttgc
     1021 ggctcatgtt gggagagcgt ggcgccgacg gcttggagca gcgcgccttc tacgatccgg
     1081 gtataggaac ccggtggtac gaccgcctga gcggcgggct catcggcgca gggctctcgg
     1141 caaacatcag aaacggctat cgctggctgg tcgagaatta cgacgagggc gatgaaatct
     1201 atctgttcgg cttcagccgc ggcgcgttcg ccgcgcgcag cctggccggc atcatcgcac
     1261 gctatggact gctgcgtgcc gatgcgccca tctcattttc gcaattgtat gagcgctacc
     1321 agaaaggcga cgcggtagcg acgatttacg aattgattcg cgccaagcat cagtccgtca
     1381 cgctcggctt cgaggatcag gcgctgctgc ggcattcgta ctaccggcgc aacatgatca
     1441 agatggtggg agtctgggac acggtcggct cgatcggatt gccgttcggc acgtttcccg
     1501 ggctgagccg gcgcatgctg aagtttcaca atacgaacct cagcgtcacc atcgagcact
     1561 cgtatcaggc actggcgatg gatgagcatc gggcacccta ttgggcggtt ctgtggacgc
     1621 atttccggcc cattgcgccg gacgtcaagc cggttgcgcg tgacgatgag cgctggatcg
     1681 aacagcgctg gttccccggc gcgcacgcca atgtcggcgg cggctaccgc ctcgatccgc
     1741 tgcccaatcg gccgctgcgc tggctgcaag ccaaggcggc ggcgtgcgga ctcgattttc
     1801 ggcgcctcgc ggatcccgcc gacgacagcc tcgcggtgat gccgcgcgat tcctatcgcg
     1861 agtttctgca cggattttgg cgaatcgcga ccttcggccg ccgttacgtg cgttgggtat
     1921 tttccgatcc cgtgcgcaag cttagcgccg ccggcacgaa ccatgcggtt ttgggttggt
     1981 tgcaaaccgt caacgaacgg atcgatgaat ccgtatggca actctgccgc gatcacccga
     2041 cgtatcggcc gctcggcctg ctcgagtggg cgcgacgcac cggacaagac ctggaggccc
     2101 tcattgcgaa ccctcaacac tggatggcgt ttagcgcggc catcgacaga cccggcatcg
     2161 agatgcgcga tccgcccccg gtcgatgagg aataaccgcc ccgcttggcg gctcgcagcc
     2221 cgcgccgccg ggggctgcgc acgcgcgtgc ggggtgctgc tgctgtgcgc gtcgagcctg
     2281 tgcagggctg catcgccgcc gcagccgtcg gcgtggaccg aaagcttcgt ctcacgcgtc
     2341 gaggcgctgg cgctgctcga gacgctgaat tcagacctct tgagccacga cagcgccacc
     2401 gcgacgctcg agcgttggtg cgcggcgcat cggttggcga cgccggctca catggtcgcg
     2461 gtgcgagttc cgaatgtcga caagccgcct tcgcctgcgc tgcgctcgct gctcaaagtt
     2521 gctccgcaaa ccgcggtccg ctatcggcgg gtgagactcg agtgcggggc gctggttctg
     2581 tcggaggcgg ataactggta tgtccccgat cgtttgacgc ccgcgatgaa cgcgctcttg
     2641 gacagtaccg agacgccgtt cggcgtcgtg gtgcgcccgc ttaatttcca acgccatacg
     2701 ctgtcctcga ccttgctctg gatgccgctg cccgcaggtt gggaaatgga tgcacacagc
     2761 caacggcagc tcggcgctga acccatacgc tcgcagatct tggaacaccg cgcgcttctg
     2821 acattgccgg acggcacgcc gttcagcacc gtgatcgaaa cctacaccaa caacgtgctg
     2881 gccttcgcgg cggtgcagcc ttgagcgcgg gacgagagcg tcgatggcgg ggacgaaccc
     2941 gaggcgcaag cgcagacgcg cgcgcatcgc gagtcgcata tttgaggcca tgcgactact
     3001 cgacaaggtt gcccgcagtc acaggccgtt cgtcttggcg ctcgaccgca tgccgggaac
     3061 ggccctcgag gtcacgggcc cgagttgcta tgcgcagcag gttgcccagt gtccattgcg
     3121 cttcgtcctc ggcgacgact tgacgcgcgc ctgcgccgaa ctcgcgttcg cggacggcac
     3181 gcggctgtcg ggatgcctgg atctgctcag aattcccgcg cgacgtctgt ggatcgaatg
     3241 gaacgatgaa gttcaccagc gggtgatctg cgagacccag tcggatgctg cgtaccctgt
     3301 tgcctgctcg gggcgccgcg tcggcgtgct cttggacggc gcggaagacg gctggcgcgc
     3361 ggtgatgaga accttttggg ccgatgcggc cgatgaagcg tccgaggcaa cgctgtcgcc
     3421 gctcgagacg catctcgact tgcgcggcgg atttaccctg ccgggcggca tcgcctgcga
     3481 cagcgggcgc gccaatgcgg cgacccgctg tctgctcgag cacgcgcgat ttcgcttcga
     3541 cgatgcgtgg gccgcgtact accgggccgc ggcggcaagt gccgtcaccc agcaagacgt
     3601 ggtgcatcgg tcggtggcgg cggtggcggc agacgcgcca ttgatactcg cgtttttctt
     3661 gctgctgagc gcgcagggcg caacgcgttc cgtgcaagtg tcgcgcgccg caatcaaccg
     3721 caagcgtctg gcgcacggcc gtccaccgct gctcgatcat atcgaggtgc gcgcttcgct
     3781 cgacgccgtt gcggccgccg aaccggccgg gctcgaaccg tcgggtcggc agtcgccgcg
     3841 cctgcatcat gtgcgcggcc atctcgtacg gcgcgaggat cgcgtctttt ggcgcgtgcc
     3901 gcacctgcgc ggcagcgcgc tacgcggcat ggttcgatcg cggaccgtgt gtctgtcctt
     3961 tgcccaacaa cgactgaact agccgccaag accgctgcgc tcgagcaagt gctcggcaac
     4021 cgcgagatcg gcgtgtgcga cgccgaccga cttgaacacc gtgcgcgatc cgcgcggtgc
     4081 ggcgcagctg gtcgcgagca gcgtattcag cgtgaaaata tcgtcagccg cgagcacgcc
     4141 tcgggcgatc ggctcggcca ggtcgccgct ctcctcgagc gcgctccgag tatcgacgac
     4201 gaccaaggca cccgtcatgc aggcgtcgtc ggcttctcgc atatcgggtc tgaaactccc
     4261 gaccaagtcc aggtgggcgc cgcgccgaag ccagccgccc tcgatcagcg gcgtctgagc
     4321 gagcgtggcg cagctcacca ccgtggcgga gcgcaccgcg atttcaagtt catcggccac
     4381 cgtgatcggc cagcctctcg aggccaggtc tcgggccacg gtcgctgcct tggttcgatc
     4441 tctgccccag atctgcaccg attcatactg ccgcacggcg aggtgaccct cgatgagata
     4501 acgcgacagg gcgcccgtgc cgaccatcag cagacgtccc gggtccgtgg gtgcgagtag
     4561 atcggcggca agtgcggaca cgccggcggt gcgcagcagg gtgagtgcgc gcccatcgag
     4621 cagagcgcgt ggttgcccgg tttgaccgga cagcaacacg taggcggcgt tgaccgacgg
     4681 cagtccgcgt gcgtcgttgt cgggaaaaac cgtgacgatc ttcacgccga ttccgccggc
     4741 aggctgccag gacggcatca gcaggaggct gcgcggagtt ttcggggagg gcagatcgta
     4801 gtgggcacgc tcgggggata tcgccggcga gccaaacgcg cggcgcggag gctcgaccaa
     4861 gctgccgacg cttgcgagcc gatgcaccgc ctcgaagtca atgtggatca tgcgccagcc
     4921 tagccgatca cattggcttc gtagagcggc cgctgcccgg caatccgcgc gtacaggaga
     4981 tccgacaaat ccaaggaatc gaagcggccg cacagaatga gctcggcgac cccgcggccg
     5041 acgatcggcg cctgctgcat gccatgaccc gagaagccgt tcatgaacca atagttctcg
     5101 agcgcagggt ggggacccag gatgccgttc tgatcgaatg cgttcatttc gaaatagccg
     5161 gcccaggcgc gctcgagccg cgcttgttcg aacgcggcaa tgcgatgcgc gagcgccggc
     5221 cagagtcgag tctcgaaggc gtcatagtcg ggctgcagcg gctcatcgtt cagagcatcc
     5281 gccggcgcgg cgcgatagct ggccgctgac aagcctgcga tgaatttcgt gccttccggg
     5341 cggatccaaa atccgctcgt atcgatcagc agcggaaacg gcgtcaactt gagcgggcag
     5401 gagatgacgt agaccgtgcg ccgctgcgca aacaccggca gctccaatcc cgccaaggcc
     5461 gcgatccgtc gcgcccaagg gcccgccgca ttgaccacgt gacggcagcc caaacgcgca
     5521 ccgtctgcca tgatcacctc ggtgacccgt cggtccgcga tgccgagact gcaaacctcg
     5581 gcattcaggt agtgcgcgcc ctgcgcccgc gctttgcgcg cgaaggccat gagcagcgcg
     5641 tagccatcaa accagccttc accgctcaaa ccaagcgtgc caaccgacac atcgcgggta
     5701 ttgagccagc cgaaccgggc tgccagttca ccgcgctcga gcagcgcgat gtcggctccg
     5761 caacttcttt gcaaggcatg cgagcggcgc agctcacgcg cattggcggc ggttgcgaga
     5821 tacaaatagc cggcctcccg caaggaaata ttcggctgtt caccatcgac tccgagctcg
     5881 agggcggcat tgcgaagaaa cgcgatactc gcctgggaga tgcggatgtt cacgggcgtt
     5941 gaaaattgct gccgaatcga cgccgccgac aaggcggacg aggcatgctg gtaggacggg
     6001 tcgcgctcga tgaccgtcac ggccagtttg ggctcgagcc gggtcagcca gtaggcgagg
     6061 gcggacccca tgacaccccc gccgatcacc gcgacttcag tgctcatcgg tggggatgat
     6121 agccacatgt gcgaattggt tctcatcccc ttagcgaccg ctcgatgacc gcacccaaaa
     6181 tgtgatccaa gccatctcgt aaattcctca attacctgag gatcttaacg tgagagcgct
     6241 gtgaaatcgc agtgcaacaa aacggcgtgg gcggcgatcg cggagggtgt cggtgcagag
     6301 cgcattagtc aaaagtcagt ccgcggcagc tcctgcggcg gggcgcaagt ccgctcgttc
     6361 gcccaaaaac gccaagctat cggcctacct cgtcacccgc gacgataccc tgtggccgca
     6421 aatcggcgcg cacatcggcc agcagctgat cctcaaacaa tccgactccg tcgacgagct
     6481 gcttgccacg acaccgcgcg aacatcccgc gatcgtcctg tgggacgcgc gcaatcagcc
     6541 cgaagccgcc atcgtgctgt cccgtctgca acagcactcg gcctgctttg ccgtcgtcgc
     6601 cctcgatgat gccggcggca gcagcggctg gcaggtggtc gtcaacgtgt cgatcccggt
     6661 catgccgcac gagttgacag ccgccctgca aatcgcaacc gaggaagttc gcgcccgcgt
     6721 ggcgctgctc ggcgagggtg gggcggaagc acacgccgcc gcgccgtctg cctccgagcc
     6781 gacggttgag gacgcatcgc acatctcggt atcggtaacg ccgagcgcag ttggcgcagc
     6841 ggagcccaag cgcgcgacgc accgcaggtc tcgcagccgc cgattcccgt ggacgccggt
     6901 gctcattgtc atcggtttgg ggatcgtctg catcgggatc tatgtggtga cgcagccgca
     6961 ggataccccg ctcaagccca cgccgcccgc gatgctgccc gccgcggtgc cggccgccaa
     7021 ggcgcctgcc gcgttgccca agttgcctgc cgcggatgac aagctcgatc tgttgatcga
     7081 gagcgcgcag aaagcgatgc tcgatcggca cttcatcgaa ccagcggagg gcagcgcgct
     7141 gtccctgtac cgcagtgtct tgctgctcga cccgagcaac ggcgaggcca atcaagggct
     7201 actgcgcctc gcggaggtgc tgttcacccg cgtacggtcc gcgctggacg aacgcaaatt
     7261 cgatgtggcg ctgcaggcgc tcgaaacggc gcgcagcatc aacccgagcg atccgcgcct
     7321 ggccgccttc gaccagcgca tcgctgcctt gcgcgccgaa ctcggtccgg cgcagattca
     7381 agcggcgatc aacgcgcaga acttcgaccg tgcatcgcaa ctgatcgacg aggcggcccg
     7441 agcgaaatcg ctgagtgcgg cgaaactcgc gcaactgcgc gacgatttgc aactcgagcg
     7501 cgaagaatcc gatgtcgcga atttcctcaa gttgatcgac tcgcgtttgc agcaggacaa
     7561 agtcatcgaa ccgcgcgagg acagcgctgc gtactatata ggcagggcgc gtgatgccgg
     7621 tgcgagcgct gccgcgctgc aggcgcaatc ccaagaagtc gacaagcggc tggcgttgac
     7681 cgtgcgtgcc gcgatcgagc agcggcgttt cgccgacgca gaacggatgc tcacctacct
     7741 ccgcgccgac ggagtcgccg cggcgacgct tgcaccgctg cagcgcgatc ttctcgccgc
     7801 gcgcactcag gcggccggac acgatcagcc gcaggcgctc gacttggcgg tgtcgcggct
     7861 cgcgcaagga aaagtaaccg agcccgacaa tgacagcgcg ttgttctacg tcaaccagct
     7921 gcgcgctgcc gatcccaaca acgccggcct gccgcgcata tccggcgcgg tccaggcgca
     7981 gattctcgag caagcgcgcg cctcgctcga tgcgactcaa gttggcgccg cggagtcgtt
     8041 gctcaagttg gccgcgggtc tcggttcctc gagcgtcttg gatacgctca atcagcggct
     8101 ggcgcaggcg aaatcgagcg ccggcgccgc gctgcctgag gtcacggaat cctcgttgac
     8161 ccggatcaac gccattgaaa ccagctatcc ggccatggca cgccgcaaga atatcgaggg
     8221 ctgggttgag ctctcctacc aggtcacggc gcagggaagc gtcgcgcaaa tcaaggtagt
     8281 gaactcgagc ccggccggga ttttcgatga cgccgcggcc aaagcactgg cgcgcgtgcg
     8341 ct
//
LOCUS       DASWZT010000325         2394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_84764, whole genome shotgun sequence.
ACCESSION   DASWZT010000325 DASWZT010000000
VERSION     DASWZT010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84764"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..1150
                     /locus_tag="VGE92_08955"
     CDS             335..1150
                     /locus_tag="VGE92_08955"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEY1313994.1"
                     /translation="MAAELSAGNLDLPSFPEIAARVRRILSDPKSTLEQVVRVVGSEP
                     ALAARLLRISNSASLNRSGRAVTDLRSAINRIGYNMVRSASISFSMAQVRKSSKLAGL
                     ERHLTELWQRSTMVAAFAYVLARDCTQVNPDEALLTGMMHAIGKLYVLTRVIDHPELF
                     ASEATLNEIIEDWHASIGKAILENWDLSDAMAQAVGDQSDVTREGEGPADLTDVIVVA
                     IIMAAYAADIAGLELALNDVGAAKRLGLDTEKTQAVMRDSAAEVTALSQALGN"
     gene            complement(1157..>2394)
                     /locus_tag="VGE92_08960"
     CDS             complement(1157..>2394)
                     /locus_tag="VGE92_08960"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF006719.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HEY1313995.1"
                     /translation="HGLYCARTRRGCRLLFQQLTRLNPDSILALMAKFRADTAPIKVD
                     LGVGVFRDLSGNTPVLACVRRAEQAVLAAQTTKSYVAAAGRAEFNEAVEAMVLGAHGA
                     RRAGRTRTVQTPGGCGALRVGAELIRAAAPATIVHVGDPTWGNHMPLLGSSGLTLERY
                     PYYDSPRHVLRFAEMLERLEHAAEGDVVLVHACCHNPTGADLDISQWRQLTELLVRRR
                     LIPFLDLAYQGFGVDLDTDVGGVRLVTEQVPEALIAVSFSKNLGLYRERVGALIAIGE
                     NENRADAVLSHMLHIARSIYSMPPDHGAAIAAHIFADAALRAQWLEELAAMRTRITDM
                     RQLLAQHLHRTTRDDSYDFIRTQRGMFSLLGVSTDTVQRLREKHHIYMLPDSRMNLAG
                     ITPDNASYVAESIAVEQRI"
BASE COUNT          453 a          741 c          777 g          423 t
ORIGIN      
        1 ccgcttcgcc ggccgctggc tgatcctcga atatatgcgc aatcccggcg cggtggcgtt
       61 ttggcgccgc gtggtggccg cttattcagc gggcagctat acggagcgct cgcgccacgg
      121 cgaggtacag cagcgtttca ccagccgggc ctcgccgacg cgctagtgcg ggcggcgcca
      181 aaagtccctc aagtatttcg tggaccggtc gatcttcaga ggctcaagcc ggagacccgg
      241 cgtgaccgcg gcattttggt aagtgagcgc aaatttaacc aataatactg attctggggc
      301 agcctccccg gtggcattcg atttcgtccg ctcaatggct gcggagttgt cggctggcaa
      361 cctggacctg ccgtcctttc cggaaatcgc agcgcgcgtc aggcgcattc tgtcggatcc
      421 aaaatcgacc ttggaacaag tcgtgcgcgt cgttggatcg gagccggcac tggccgcgcg
      481 gctgctgcgc atttcaaatt ccgcctcgct caatcgcagc gggcgggcgg tgacggatct
      541 gcgttcggcg atcaaccgca tcggctacaa catggtgcgc agcgcctcga tctccttctc
      601 catggcgcag gtgcgcaaga gcagcaaact cgcgggcttg gagcgacatc tcaccgagct
      661 ctggcaacgc agtacgatgg tggcggcgtt cgcctacgtg ctggcgcgcg actgcacgca
      721 agtcaatccc gatgaggcgt tgctgaccgg catgatgcat gccatcggca agctgtacgt
      781 attgacgcga gtcatcgatc atccggaatt gttcgcgagc gaagcgaccc tcaacgaaat
      841 catcgaggac tggcatgcct cgatcggcaa ggccattctc gaaaactggg atctctccga
      901 cgccatggcg caggcagtgg gcgatcagtc ggatgtgacc cgcgagggcg aagggccggc
      961 agatttgacg gacgtcatcg tcgtcgcgat catcatggca gcgtacgccg cggatatcgc
     1021 cggcctcgag ctggcgctga acgacgtcgg cgccgccaaa cgcctagggc tcgataccga
     1081 gaagacccag gccgtgatgc gcgactccgc ggccgaggtc acggcactga gccaggcact
     1141 cggcaattga gcgcggtcaa atgcgctgct caaccgcgat cgactccgcc acgtaactcg
     1201 cattgtcggg cgtgatgccg gcaagattca tgcggctgtc cggcagcatg taaatgtgat
     1261 gtttttcgcg cagccgctgc accgtgtcgg tcgaaacccc gagcagagaa aacatgccgc
     1321 gctgcgtgcg aatgaaatca taggaatcgt cgcgcgtggt cctgtgcaga tgttgcgcga
     1381 gcagctgacg catgtcggtg atgcgggtgc gcatggcggc gagttcctcg agccattgcg
     1441 cccttagggc ggcgtcggca aagatatgcg cggcgatcgc ggcaccgtgg tccggcggca
     1501 tcgaatagat gctgcgcgcg atgtgcagca tatggctcaa gacggcgtcc gcgcgattct
     1561 cgttctcgcc gatcgcaatg agcgcaccga cccgctcgcg gtacagaccc aaattcttgg
     1621 aaaaagaaac tgcgatcagc gcttcgggga cctgttcggt gaccagacgc acgccgccga
     1681 catcggtgtc gagatcgacg ccgaagccct ggtaggcgag atcgagaaac ggaatgagcc
     1741 ggcggcgcac caacagctcc gtcaattgcc gccattgcga aatatcgaga tccgctcccg
     1801 tgggattgtg acagcaggcg tggaccagga cgacgtcgcc ctccgccgcg tgctcgagcc
     1861 gttcgagcat ttcggcgaag cgcagaacgt gccgaggcga atcgtagtac gggtagcgtt
     1921 cgagcgtgag gcccgagctg cccaacaagg gcatgtggtt gccccaggtt ggatcaccga
     1981 catgcacgat cgtcgccggc gccgcagcgc gaatcagttc ggcgccgacg cgcagcgcgc
     2041 cgcaaccgcc gggcgtctgt acagtacgcg tgcgaccggc gcggcgggcg ccatgggcgc
     2101 ccaacaccat ggcctcgacg gcctcgttga attccgctcg accagcggcc gccacgtagg
     2161 atttggtggt ctgtgcggcg agtaccgcct gctcggcgcg ccgcacgcag gcaagcaccg
     2221 gtgtgttgcc gctcaggtcg cggaaaacgc ccacgccgag atcgaccttg atcggggcgg
     2281 tatctgcccg gaatttcgcc atcaaagcca ggatcgaatc cgggttcaaa cgcgtcagtt
     2341 gctggaacaa gagccggcac ccccttcttg tgcgggcaca gtataggcca tggc
//
LOCUS       DASWZT010000326         3715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_94620, whole genome shotgun sequence.
ACCESSION   DASWZT010000326 DASWZT010000000
VERSION     DASWZT010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94620"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /gene="purM"
                     /locus_tag="VGE92_08965"
     CDS             complement(<1..663)
                     /gene="purM"
                     /locus_tag="VGE92_08965"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEY1313996.1"
                     /translation="MTERAPVTYREAGVDIDAGEDLVERIKPHVRRSMRREVLGGLGG
                     FGALVEVPLDRYRKPVLVSGTDGVGTKLRLAIDTERHDTVGIDLVAMCVNDVVVQGAE
                     PLFFLDYFATGKLDVGVGERLIAGIVEGCVQAGCALVGGETAEMPGMYHGADYDLAGF
                     CVGIVEKDALIDGSATRAGDVVLGLPSSGAHSNGFSLIRKILQVSGADLNSSLDGLTL
                     VDR"
     gene            740..1768
                     /locus_tag="VGE92_08970"
     CDS             740..1768
                     /locus_tag="VGE92_08970"
                     /inference="COORDINATES: protein motif:HMM:NF021367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HEY1313997.1"
                     /translation="MPHYFSVFCRRKLRRAVFLCSIGVIFCWSHAAHALTRTELYQAS
                     APVSDRSEAAQAAAFQNAMKIVLIRVTGRRTADEEPALAPLVSNARRYVQQYRAAPDG
                     QLLVSFDGQAIERWLTQNGQPLWGRERPATFVWLAAQTGAQSGTVITADDTSDVKMAI
                     DAAADLRGVPLLWPSAVDVQKNRLDYAGISGGSAATLADMAHRLGGEAVLIGRASATS
                     ANANVRWTHLFQDRSSEFTGALEGVNGAADLYAGLFAASGNLAPMDIEVTGIADLREY
                     ANVQTYLESLSFISHVNVIALSGDTVRFRLATRGGADSLQRTLSLSGRLQPIAAGDTG
                     ILRFQLRR"
     gene            1773..2363
                     /locus_tag="VGE92_08975"
     CDS             1773..2363
                     /locus_tag="VGE92_08975"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY1313998.1"
                     /translation="MTWSARQIPNVITSIRILLVAPIGVALAEHRLAISIVLFGVAAA
                     SDAADGFLAKRYGWQSDLGAVLDPLADKLLLVTVFIVLAYLQLVPLWLMAVAVARDVI
                     IVSGALLYRYCIGPLTVRPSSVSKFNTLCQAAFIMAIVGRQEFAVPSAQTVMVLGALV
                     FVTVVVSGIDYVLIYGRRALNLARPRAAARAGDRRS"
     gene            2360..3043
                     /gene="hda"
                     /locus_tag="VGE92_08980"
     CDS             2360..3043
                     /gene="hda"
                     /locus_tag="VGE92_08980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEY1313999.1"
                     /translation="MKQLALGVRLRADAVFASFWPGANGEALAALQAPGVTPLWLWGA
                     AGSGKTHLLQAVCAATGEGAAYFPLATLGLPADSLAGFERSRVLCVDDVDAVAGDLAW
                     EKALFRLFNEAAELRTRLIFAAAAAPRQLDWSLEDWRSRAAACVVYQLRELDDPGRIE
                     ALRLRAAQRGLQLPVETSEYLLKRMPRDLPSLFDILDQLDEASLAAQRRLTIPFIRDA
                     LEKHVRTKS"
     gene            complement(3000..3335)
                     /locus_tag="VGE92_08985"
     CDS             complement(3000..3335)
                     /locus_tag="VGE92_08985"
                     /inference="COORDINATES: protein motif:HMM:NF021369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEY1314000.1"
                     /translation="MSFESVVRSLTLWLWTAVVLSLLAWMMVGYPWPICLLAALPLLV
                     PLNGLLRGRRHTYAWATLFAIPYLVFALTELLVNPAARWVAGMSLFLVFGWFCTMILF
                     LRASRAHRG"
     gene            complement(3332..>3715)
                     /locus_tag="VGE92_08990"
     CDS             complement(3332..>3715)
                     /locus_tag="VGE92_08990"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY1314001.1"
                     /translation="ILGSPTRFGNMAAPLKFFLDGTSALWLSGALAGKPAGVFTSTQT
                     LHGGQESTLLSMMIPLLHHGMYLVGLPFTERGLTDTRAGGTPYGASHVASVSGHGELS
                     QTERELARALGQRVAALAARLQNHA"
BASE COUNT          654 a         1166 c         1207 g          688 t
ORIGIN      
        1 ccgatcaacc agggtgaggc cgtcgaggga actgttgagg tcggcgcccg acacttgcag
       61 gattttgcga atcaacgaaa agccgttgga atgagctccc gaggagggca acccgagcac
      121 cacgtccccg gcgcgagttg ccgagccatc gatgagcgca tccttttcga cgattccgac
      181 gcagaatcca gccagatcgt agtcggcgcc gtgatacatg ccgggcatct cggccgtttc
      241 gccgcccacc agcgcgcagc cggcctgaac acaaccttcc acgattccgg caatcagccg
      301 ttcgccgacg cccacatcga gtttgccggt cgcgaagtag tcgagaaaaa acaggggttc
      361 cgcgccttgc accaccacgt cattgacgca catggccacc aagtcgatac ccacggtgtc
      421 gtgtcgttca gtgtcaatcg cgagccgcaa tttggtgccg acgccgtccg tcccggagac
      481 gaggacgggc ttgcgataac gatcgagcgg aacttccacc aaggcgccga agccgccgag
      541 gccgccgagc acctcgcggc gcatcgaacg cctgacgtga ggcttgatgc gctcgacgag
      601 atcctcaccg gcatcgatgt cgactcctgc ctcgcgatag gttaccgggg cgcgttcagt
      661 cattcgattc ctcgttcgtg gaagggcggg ccccacccgg tcggcatcgg ataggccgtg
      721 tggtatttta cctcacacca tgccacatta tttcagtgtc ttttgccgcc gcaaattgcg
      781 ccgggccgtg tttttatgct cgatcggcgt cattttttgt tggtcacacg cggcccacgc
      841 gctgacccgc acggagctgt accaggcaag cgcgccggtg agcgatcgct cggaggcggc
      901 gcaagccgcg gccttccaga acgccatgaa gatcgtgctg attcgggtca ccgggcggcg
      961 caccgcggat gaagagccgg cgctcgcgcc gctcgtcagc aacgcccgcc gctatgtcca
     1021 acagtaccgc gcggcgcccg atggccagtt gttggtatcc ttcgacggtc aggccatcga
     1081 gcgctggctc actcaaaacg gccagccgct ctgggggcgt gaacgaccgg cgaccttcgt
     1141 gtggctggcc gctcaaacgg gcgcgcaatc gggcacggtc atcaccgccg acgacacatc
     1201 ggacgtgaag atggcaatcg atgcggccgc tgatttacgc ggtgtgccgc tgctctggcc
     1261 gagcgccgtc gacgtgcaga agaatcgctt ggactatgcg ggcatcagcg gcggctccgc
     1321 ggcaacgttg gcggatatgg cgcatcgctt gggcggggag gcggtactca tcggccgggc
     1381 ttcggcaacg agcgcgaacg ccaatgtgcg ctggacgcat ctgttccagg accgcagcag
     1441 cgaattcacc ggcgctttgg aaggagtgaa tggtgcggcc gacttgtacg ccggactgtt
     1501 cgcggccagc ggcaatttgg cgccgatgga tatcgaggtg acgggcatcg ccgatttgcg
     1561 cgaatatgcg aacgtgcaga cttacctcga atcgttgtcg ttcatctcgc atgtgaatgt
     1621 catcgccttg agcggcgata cggtcagatt tcgcctggcg acacgcggcg gcgccgactc
     1681 gttgcagcgc actttatctt tgagcggccg tctgcagccg atcgccgccg gcgacacggg
     1741 aattttgcgc tttcaacttc gccgttaaac gcgtgacctg gtctgcgcgc caaatcccca
     1801 acgtcattac ctcgatcaga atcctgctgg tcgcgccgat cggcgtggcg ctggccgagc
     1861 atcggctcgc gatcagcatc gtgctcttcg gagtggctgc tgcctcggat gccgccgacg
     1921 gctttctcgc caagcgctac ggctggcaaa gcgatctcgg tgcggtgctc gatccgttgg
     1981 cggacaagtt attgctggtg accgtgttca tcgtgctcgc gtatttgcag ctggtgccgc
     2041 tgtggctgat ggcggtcgcg gtggcgcgtg acgtcatcat cgtgtcgggg gcactgctct
     2101 accgatattg catcggaccg ctcacggtgc gtccgtcgag cgtgagcaag ttcaacacgc
     2161 tgtgccaggc cgctttcatc atggccattg tcggcagaca ggagttcgcg gtgccctcgg
     2221 cgcagacggt gatggtgctc ggggcattgg tgttcgtgac cgtggtggtc agcggcatcg
     2281 actacgtttt gatttacggc cgccgcgcat tgaatctcgc gcggccgcgg gccgctgcgc
     2341 gcgccggcga ccggcgctcg tgaaacagct ggccctgggt gtgcggctgc gagcggatgc
     2401 ggtattcgca agcttctggc cgggcgcgaa tggcgaagcg ctcgcggcgc tgcaggcgcc
     2461 gggcgtcacg ccgctgtggt tgtggggcgc ggccggctcc ggcaagacgc atttgctgca
     2521 ggcggtgtgc gccgcgaccg gtgagggggc ggcgtatttt cctttggcga cgctcggctt
     2581 gccggccgac tcactggcgg gcttcgagcg cagtcgcgtg ttgtgcgtcg atgacgtcga
     2641 tgccgtggcc ggcgatttgg cgtgggagaa ggcgctgttc cgcctgttca acgaagcggc
     2701 ggaattgcgt acccgcttga tctttgccgc cgccgctgcg ccgcgacaac tcgattggtc
     2761 gctcgaggac tggcgctcgc gcgctgctgc ctgcgttgtc tatcagttgc gcgagctcga
     2821 tgacccgggc cgcatcgagg cgctgcgttt gcgcgcggcg cagcgcggct tgcagcttcc
     2881 cgttgagacg tccgaatacc tgttgaagcg catgccgcgg gacctgccct cgttattcga
     2941 catcctcgat caactcgacg aggcgtcgct ggcggcgcag cgccggctga ccattccgtt
     3001 tatccgcgat gcgctcgaga agcacgtaag aacaaaatca tagtgcagaa ccagccgaat
     3061 accagaaaca ggctcatacc cgccacccaa cgcgcagccg ggttcaccaa cagttccgtc
     3121 agggcaaaca ccagataggg aatggcaaat aaagtcgccc acgcgtaggt gtggcggcgg
     3181 ccgcgcaaca gtccgttgag cggcaccaac aagggcagag cggcgagaag acaaatcggc
     3241 catgggtagc cgaccatcat ccatgcgagc aaactcaaca caaccgcggt ccacagccag
     3301 agcgtcagcg agcgcaccac cgattcgaag ctcatgcgtg attctgtaat cgagccgcaa
     3361 gcgcggcgac gcgctgaccc aacgcgcggg cgagctcacg ttcggtctgc gacaattcac
     3421 cgtggccgct gaccgacgcc acgtggcttg caccataggg tgtgcccccg gcgcgcgtgt
     3481 ccgtcaagcc gcgctcggta aagggcagac ccaccaggta catgccgtgg tggagcagag
     3541 gaatcatcat cgacagcaat gtcgactcct ggcccccatg cagggtttgc gtcgaggtga
     3601 acacgccggc cggtttaccg gccaatgccc ccgagagcca gagcgcgctc gtgccgtcga
     3661 ggaaaaattt gagcggcgcc gccatattgc cgaaacgggt cggacttccc agtat
//
LOCUS       DASWZT010000327        18366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_31757, whole genome shotgun sequence.
ACCESSION   DASWZT010000327 DASWZT010000000
VERSION     DASWZT010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 18366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18366
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31757"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VGE92_08995"
     CDS             <1..352
                     /locus_tag="VGE92_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEY1314002.1"
                     /translation="ASGMTVIYMEDFMRLGVAAVAERARAVVGSGPVYISVDVDGFDP
                     AYAPGTGTPEAGGLEAREGLALLRALGGLHVIGADVVEVAPQYDPTTNTSQLAAQLLF
                     EEFALMTLGSAAPA"
     gene            complement(422..2743)
                     /locus_tag="VGE92_09000"
     CDS             complement(422..2743)
                     /locus_tag="VGE92_09000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY1314003.1"
                     /translation="MALSAAPAGGLTSVEAQTRLKSSGPNSMPDPALHPLSMALAKFW
                     APVPWMLEATIALELILGKYVEAAIIALLLIFNALLGFLQESRAQATLAALKSRLALS
                     ASVLRDNVWKTVPAAELVQGDVVKLSLGAVVAADVRICTGEVLLDQSMLTGESVPVEA
                     GAGVQSYAGALVRRGEAVAEVTATGTRTKFGRTAELVRTAHVESSQQKAVFRVVRNLA
                     FCNGVTILLMVGYAYFLKMPVAEIIPLALTAILASIPVALPATFTLAAALGARALATL
                     GVLPTRLSAVDEAASMDVLCADKTGTLTQNELTVMSVHPLSGFDEAHLLVFAALASSE
                     GGQDPVDAAIRSAAATKKVPETMHLTAFTPFDPANKRSEATFTDAKGTPQRVAKGAFS
                     EIVGLVPVEASAASAANDLEGKGYRVLAVAAGSATSMKMVGLIALSDPARADSPGLVT
                     ALQALGVRTIMVTGDAPATAAIVAHTVGLTGAVSPPGIATDVRPEQYSIYAGVLPEDK
                     YKLVKAFQKSNHTVGMCGDGANDAPALRQAQIGIAVSTATDVAKAAAGMVLTKQGLEG
                     IVAAVNEGRLTFQRILTYTLNSITKKIAQVLFLAVALIMTGHAILTPMLMVIIMITGD
                     FLGMSLTTDRVRPSQSPNAWRIRSLTVAGALMGIGELIFVTVILAYGKYAMNMDLSTL
                     RTLAFLAIVFGNQAITYNNRERRHLWSSRPSHWVLGSSGVDILIATTLSVMGIAMRPL
                     PIRMVIGTLAGAAAFAVVLDFVKVPAFRRLAIT"
     gene            complement(2810..4273)
                     /locus_tag="VGE92_09005"
     CDS             complement(2810..4273)
                     /locus_tag="VGE92_09005"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEY1314004.1"
                     /translation="MTLPRREFLKLSGLAAIGSSLGPWSKSTAQTSSAAETKADYALR
                     IATGLVDLAPEHIVSTTLYNDQFPGPLLRFKEGQRTVIDISNDTDTPELVHWHGQRIA
                     SDVDGASEEGTPFVPAHAMRRVSFVPEPSGFRFYHTHVLAGGDLNRGTYTGQAGPVYI
                     DPKNNPGAYDREIFLVMKEFEPSFSRGGDMAMDFLAGEPLKELQQIGKRADAEAKEKA
                     KGFEVGYARFGINGKMLGSGDPVRVKQGERVLFHVLNASAGEIRSLALPGHSFRVVAL
                     DGNPVPTPTEVPVLWLGTAERICAIVEMNHPGVWVLGDLDDDDRRHGMGIVVEYAGRS
                     GKAQWIKPKPFRWDYARFGKITGEPAHPDETIEMTIVKNNAALNGFNQWTLNGAAFSM
                     QTMKPMVTLHQGGRYRLKFRNASDDIHPLHLHRHSFELTQIGGKATSGVVKDVVMLAG
                     FQELSCDFVADNPGLTLFHCHQQLHMDFGFMALFKYA"
     gene            4478..4846
                     /locus_tag="VGE92_09010"
     CDS             4478..4846
                     /locus_tag="VGE92_09010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HEY1314005.1"
                     /translation="MNTRNLAKKIAVATVAALGVACATGAHADAALVNAPTRTVHYAD
                     LNLNTQAGAALLYKRIRVAAEEVCGDVNSRRLDEAAMAKACVDRAVYGSVQTVNNVKL
                     TNEYNAHTGAQKSINVAALR"
     gene            4986..5603
                     /locus_tag="VGE92_09015"
     CDS             4986..5603
                     /locus_tag="VGE92_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314006.1"
                     /translation="MLHFIRGRYMNNLPVNAVVLAVALIAVAVVLVAWFAIQKQRTSR
                     LKQRFGSEYGHAVSEFGSRSKAEAELRKREVRVERLNIAPLTAADATRFAQAWSALQG
                     RFIDNPKGVVVEADLLVRELMVKRGYPMSDFERRAADISVDHPDVVTTYRTAQAIAQR
                     DQRGEADTEELRKAVVHYRTLFDELLEVKSTPSTDLAPARIPVHS"
     gene            5615..5818
                     /locus_tag="VGE92_09020"
     CDS             5615..5818
                     /locus_tag="VGE92_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314007.1"
                     /translation="MRKPSTPAADFPTAESAEQLPSDFAIAPRTTGTVDDDDAPADER
                     PDENEARVREKSESTLDGGVAAE"
     gene            complement(5836..6354)
                     /locus_tag="VGE92_09025"
     CDS             complement(5836..6354)
                     /locus_tag="VGE92_09025"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY1314008.1"
                     /translation="MPTYFRARETAREDAIDGVQLAPFTRRAIGFGIDFVLVSMLRKP
                     AEFLWENYFPHQWERHTLINLPHAVDVIVLVLYFWVALYIGNGQTIGKRLMRIKVISL
                     THERITFWQALERSLGYGASFLEAGFGFAQFFLQRNRQCAHDRLAETIVIDAGKSATR
                     RDDFVEPGRGPD"
     gene            complement(6375..7256)
                     /locus_tag="VGE92_09030"
     CDS             complement(6375..7256)
                     /locus_tag="VGE92_09030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY1314009.1"
                     /translation="MRRLLSTLRDAKDIVAQAALAWIRHDASTMGAALAFYTVFSIAP
                     ILVIAIGVVGLAIGTDTVQAGLLPQMQVLFGDAGASAVQTLLLSANHMGKSRLATLIG
                     VVVLLMGASSVFVELQNSLDRIWGAPARSRAGGLWQVVRARFLSLGLVFGVGFLLLVS
                     LLVSTILTALDTWIASFLGEWRSLLPLLDLTVGLAITTVLFALVYKYIPQLKIEWRDV
                     WVGALVAASLFNIGKLAIGYYLGRSAFAPIYGAAGSLLVLLLWAYYSAQIFLFGAELT
                     KSFSFVRGTRRGLAPAA"
     gene            complement(7319..7864)
                     /locus_tag="VGE92_09035"
     CDS             complement(7319..7864)
                     /locus_tag="VGE92_09035"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEY1314010.1"
                     /translation="MNKLLSVAALGVSLSTLAVGTVLADTKPELDVSVHETIKAFDAL
                     NPNNRQLLHSAAGTLVFPRVTKAGAGVGGEYGEGALRIDGKTVGYYSVSSASIGLTLG
                     ASKRSEVIVFTTQESLNQFTSSKGWSIGADTGVAMISKGSGANYDTDTLKKPVVGFVF
                     AEKGLIGDLSLAGSKIDKIDR"
     gene            complement(7980..8279)
                     /locus_tag="VGE92_09040"
     CDS             complement(7980..8279)
                     /locus_tag="VGE92_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314011.1"
                     /translation="MEIDESPEKFDTGLRRIGHEIKSRLVLHGLFGTIDHGRPSDIPG
                     GTRVEIHAKGRTVGRSFNRQQIEGCCLRVGGTVLAGIIAMVDELADRSEPATARD"
     gene            8495..8623
                     /locus_tag="VGE92_09045"
     CDS             8495..8623
                     /locus_tag="VGE92_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314012.1"
                     /translation="MNARIMIGLALVVGLAAHASTPERRVEVVIGDDNGAATAARS"
     gene            8632..17193
                     /locus_tag="VGE92_09050"
     CDS             8632..17193
                     /locus_tag="VGE92_09050"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEY1314013.1"
                     /translation="MLRFGSPVSPWDNDEILRAELFSSERLEQHAASLAAAQQVTRRR
                     SARRSLNARLKENESVLLSAYRAIATAVGEGRSITPSAEWLLDNYHLVEEQIREIRAD
                     LPPGFYRQLPKLAAGPFVGYPRVFGLAWAYIAHSDSRFDPESLRLFVRAYQRVQPLTI
                     GELWAVAITLRIVLVENLRRAAVRIVSSRAAREDADTVADRLLGVNGYAAEPDALARR
                     VATGASFAPPFVVQLVLRLRDQDPDTTPARQWLDTFVNAQGSTADEMVHGEHQRQGAS
                     NVTVRNIITSMRLLSDVDWPELFESVSLIDELLRSKSGFAAMDFASRDLYRRAIEQLA
                     RGSKKTELAIACAALAAGDADPAIDGLDVERKRDPGYHLIGAGRRAFEASVGYRPSRW
                     NSVARFTTQGAGEYIGAVALIAAMILAVPLLEMQAWGVGNAYLALLAVLGLVPSLDAA
                     MALVNRAVTKGRGATILPGLALREGIPPHLRTMVVVPTLLTTRAAIEEHIELLEIHYL
                     ASPDGDLFFALLSDWMDAPAQHTAADTQLLDIAIEGIARLNRRHGAAPSGDRFALFHR
                     RRVWSEGQRQWMGWERKRGKLHELNRLLRGAPDTTFVELGGRPPVIPAQVRYVVTLDA
                     DTRLPRDTVRRLVGKMAHPLNQPRFDAGTGQVVEGYAVLQPRVAPSLPTGREGSLFQR
                     VFSSTGGIDPYAAAASDVYQDLFGLGSYAGKGIYDIDAFEAALAGRVPEGTLLSHDLF
                     EGTFARAGLVSDIEVVEEFPSRYDVAAARQHRWSRGDWQLLPWILGRSDAAGDDRGSG
                     ALPLIGLWKMLDNLRRSLSAPAAVAALLAGWLLPTASAVLWSAFISATVALPPLLPIF
                     TGILPSRAGVTLRSHLRALRRDVGLGAAQTAFILTFLAHQASLMVDAIGRTLFRLLRR
                     RNLLEWVTAAQARLKSSLTLSSAYVGMAGAVAIAAIDAVIVGHFRADNVWLAAPFLVL
                     WAASPAVAVWISMSPLVAGRLTVSAADARALRLVARRTWRYFETFVTAEDQNLPPDNF
                     QEDPRPVIAHRTSPTNMGLYLLSAVSARDFGWSGTLDFVDRVEATFATMLRLERFHGH
                     FYNWYDTRDLRPLDPRYISTVDSGNLAGHLIALANACHERIDLPLRCGESLAGIRDSL
                     NLAREARLELPDDRRMHATSRDQIDAAIAALAAALMQAEAATVEIATQWSTLMPLAVT
                     LVDIVSTLAGEWSGDAAGELLYWVEATKRSVESRRRDVMQTPDSLDDLQARLAAIEVS
                     ARAMADAMQFGFLFDPQRRLLSIGCTADAALDSNCYDLLASEARLASFIAIAKNDIPA
                     RHWFRLGRSVTPIGRGAALISWSGSMFEYLMPSLVMRAPAGSLLEQTNRLVVQRQIRY
                     GIELRIPWGISESAYNARDLELTYQYSNFGVPGLGLKRGLSENTVIAPYATALAAMVD
                     PQRAVRNFERLNVIGARGQYGFHEALDYTRSRVPDGAEVAIVRAYMAHHQGMTIAAIA
                     NVLHDGQMRARFHAEPSIRATELLLQERAPRDVSVVHPRAEEVKTAAPLLDPRVPDVR
                     RLHSPFDATPQTHLLSNGRYSVMITGAGSGYSRWRDIAVSRWREDATRDDTGSYIYLR
                     DVASGAVWSAAFQPSGVEPDSYLVTFTEDRAEFIRTDATLTTTLEIVVSPEDDAEVRR
                     VSIANSGYRDREIEVTSYFELVLALPAADAAHQTFSKLFVQTEYIAKRNTLLATRRKR
                     TPDEPDIWVGHHAVIEGSVLGEAEIETDRARFLGRGQEVRSPLAVLDGRRLSGSVGAV
                     LDPVFALRYRLNVAAGTTARIAFWTGVEASRSALLDLLDKHHEANSFTRAATLAWTQA
                     QVQLRHLGVDSAEAGLFQRLAGHLLYADASMRSPANTIRDGSGPPSGLWAQRISGDIP
                     IVLLRIDAVEDIALARQVVRAHEYWRMKGLATDLVILNERAASYVQDLQNALETLVRM
                     SQSRPQPGAHSVRGAVFVLRADLISEQTRSLLSAVARVVLVARRGGLADQLDRGRKPV
                     GLAPVARKRVLTSEAPMSADAGHDLEFFNGLGGFGAQGREYVTLLGPGQSTPAPWINV
                     IANPHFGFQVAADGSGFTWALNSREHQLTPWSNDPVADRPGEVLYVRDEESGEVWGPT
                     AAPIRDPGATYTATHGQGYSRFELTARDIALDLCLFVAMDDPIKICRLRIRNTSRRSR
                     RLSLTAYVEWVLGTSRGSSAPFIVTELDTDTGALLARNPWDAAFGSRVAFADLAGRQS
                     QWTADRGEFLGRHGTLDMPAAMSTRAPLAGRAGAGLDPCCALRTMVDLEPGATSEVVF
                     FLGEADNASRARSLLTRYRGADLDAVFRKVCEHWDSVLGVVQVKTPDRSLDIMLNRWM
                     LYQTLACRIWARSAFYQASGAYGFRDQLQDGMALSVARPDLTREHLLRAGARQFGAGD
                     VQHWWLPPVAAGATGQGVRTRISDDVVWLAYCAVQYIESTGDAAVLDEAIPFLEGRSL
                     QPDEHDAFFMPGISEDTATLFEHCARALDHALPVGEHGLPLIGTGDWNDGMNRVGEKG
                     KGESVWLGWFLNATLSSFAPLAAARGEQVRASKWRAHAAALQRSIEQAWDGDWYRRGY
                     FDDGTALGSAASTECRIDSIAQSWSVMSGAGDPGRQSRAMANVQSQLVRPDVGLVLLF
                     TPPFDHTAHDPGYIKGYPPGIRENGGQYTHAAAWTVIAEAMRGHGAEAAKLFSFLNPI
                     NHTSSRADVQRYKVEPYAVAADVYSVAPHVGRGGWTWYTGSAGWMYRAGLEWILGFRV
                     RAGTLLVTPSIPADWPRIEIVFSHASALYEILIENPHGVSRGVAYVELDGIRLGEHHI
                     VLADDGRRHAVRVVLGEASSVTTYA"
     gene            17313..18014
                     /locus_tag="VGE92_09055"
     CDS             17313..18014
                     /locus_tag="VGE92_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314014.1"
                     /translation="MGELIARLTGIDETSRTQHAFLQEGDRCLFFAEYFTGKGYQAGG
                     INQLMFNFKTLPSIASDNPLRRLHRERAISTIATGLREVLGRKQIERLTFVPVPPSKA
                     LGHSDYDDRLTRTLKLGCTGCDADIRPLLRRSSSTEPDHRSGDRLTPDELHALLALDF
                     AALAAKPLREVVVVFDDVITTGKSFKCCERRLREVIPATVPIIGLFVARRIPHTPFDA
                     LEHVFASRAHLGNSL"
     gene            18085..18321
                     /locus_tag="VGE92_09060"
     CDS             18085..18321
                     /locus_tag="VGE92_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314015.1"
                     /translation="MKMLSVLSKSDVRGLLSSLLLGLMALPAAAGSVTDANYTISASA
                     SGTQLTTTVPGAATFADSSTLAGQHPRRRGHRGQ"
BASE COUNT         3450 a         5711 c         5728 g         3477 t
ORIGIN      
        1 tgccagcggc atgaccgtca tttacatgga agacttcatg cgcttagggg tggcggcggt
       61 agccgagcgg gcccgcgcag tcgtgggctc ggggccggta tacatatcgg tcgacgtcga
      121 tggcttcgac ccggcgtatg cacccggcac cggcacaccg gaagctggag gcctggaagc
      181 gcgggaaggg ttggccttat tgcgcgcact gggcggtctt cacgtcatcg gtgcggatgt
      241 ggtcgaggtc gcgcctcaat acgacccgac cacgaacacc agtcaactgg cggcgcaatt
      301 gctgttcgag gagttcgcgt tgatgacgct gggatcggcc gctcccgcct gaaggtgcgc
      361 cggaagaccg actaccgccc ttgcagttag ctgcctaaag gcggccgtcg agcgagcgtc
      421 gctaggttat ggcgaggcgt cggaacgcgg ggactttcac gaaatccaat accacggcga
      481 acgccgcggc gccggcaagt gtacctatga ccatccggat gggcagaggc ctcatcgcga
      541 tacccatcac cgacagtgtt gtggcaatca gaatatcgac gcctgaagac cccaagaccc
      601 agtggctagg tcgggacgac caaagatgtc ggcgctcgcg gttgttgtag gtgatcgcct
      661 gattgccgaa gacgattgcc aggaacgcga gcgttctcag tgtactcaag tccatgttca
      721 tggcgtactt gccgtacgcg aggatgactg taacaaatat cagctcgccg attcccatca
      781 acgcgccggc gacggtcagg ctgcgtattc tccaggcatt ggggctctgc gagggccgta
      841 cgcgatccgt ggtcaaagac atcccgagaa aatcgcctgt aatcatgatg atgaccatca
      901 gcataggcgt gagaatcgcg tgtccggtca tgatcaacgc cacagccaag aacaacacct
      961 gagcaatctt tttcgtgatg gaattcaacg tgtaagtgag gatgcgctgg aaggtcaggc
     1021 ggccttcatt gacggccgcg acgattccct cgagaccctg cttggtgagc accatgccgg
     1081 ctgcggcttt cgccacatcc gtggcggtcg agacggcgat accgatttgc gcttgtctga
     1141 gtgccggcgc atcgttcgcc ccgtcgccgc acattcccac ggtatggtta ctcttctgaa
     1201 aggccttgac gagtttgtac ttgtcctccg gcagcacgcc tgcatagatg gagtactgtt
     1261 caggccgcac atcggtagca attccgggtg gcgaaacagc gccggtgagc cccacggtgt
     1321 gggcaacgat cgcagcagtc gctggcgcat cgcctgtgac catgatggtg cgcacgccca
     1381 acgcctgtaa cgcggtcacg agacccggcg agtcggcgcg cgcgggatcg ctgagcgcaa
     1441 tgagtccaac cattttcatg ctggtcgcgg agccggcggc tacggcgaga acccgatagc
     1501 ccttgccctc gaggtcgttt gcagcgctgg cggcgctcgc ctcaacgggc accaagccga
     1561 cgatctccga aaaagccccc tttgccacgc gctgcggcgt acccttcgcg tctgtaaacg
     1621 tcgcctcgga tcgcttgttc gcgggatcga acggtgtaaa ggcggtcaga tgcatcgtct
     1681 cagggacttt ctttgtcgcc gctgccgagc gaattgccgc atcgaccgga tcctggcctc
     1741 cctcggaact cgccagggcg gcaaacacaa gcagatgtgc ttcatcgaat cctgataaag
     1801 ggtgaacgct catcacggtc agttcgtttt gggtcaacgt acccgtttta tcagcgcaca
     1861 acacatccat cgatgcggcc tcatcgacag ccgaaaggcg ggtcggaagc accccaagcg
     1921 tggccaaggc ccgtgcgccg agcgcggcgg cgagtgtaaa cgtggcaggc agcgctaccg
     1981 ggatggacgc cagaatcgcg gtcagcgcca ggggaatgat ttcggcgacc ggcatcttca
     2041 gaaaataagc gtagccgacc attaaaagaa tggtgacccc attgcagaac gccagatttc
     2101 gaacgactcg aaaaacagct ttttgctgag agctctcgac atgggcggtg cgcacgagct
     2161 cggcggtgcg accgaatttg gtccgcgtgc cggttgccgt cacctcggcg actgcctcgc
     2221 cgcgtctgac caaggcgccg gcgtaagatt gcacccctgc acctgcctcg acaggaactg
     2281 actctccggt cagcatcgat tggtcaagca gaacttcccc ggtgcagata cgcacgtccg
     2341 ccgcgacgac ggcgcctagt gatagcttta cgacgtcacc ttggacgagt tcagccgcag
     2401 gcaccgtctt ccacacgttg tcgcgtagca ccgaggcgct caatgcaagg cgggatttca
     2461 gggcggcaag agttgcctga gcccggctct cttgcaagaa gccaagaagt gcgttgaata
     2521 tcagcagcag ggcaatgatc gcggcttcga cgtacttgcc gagaatcagt tccagcgcaa
     2581 tcgtcgcctc gagcatccat ggaaccggcg cccagaactt tgcaagcgcc atactcagcg
     2641 gatgaagcgc ggggtctggc atcgaattcg gtccggagct ctttaggcga gtttgtgcct
     2701 ctacggacgt aagacccccg gcgggcgcgg cgctcaatgc cacgagcgca ggcaacaatt
     2761 cattttcgca gtcaggtgcg gctccttacg ccaacactcg tgccatcgct cacgcatact
     2821 tgaacagcgc catgaatccg aagtccatgt gcaattgctg gtggcagtga aacagcgtca
     2881 atcccggatt atcggcgacg aaatcgcagg atagttcctg gaagccggcg agcatgacga
     2941 cgtctttgac cacacccgaa gtggccttgc cgccgatctg cgtcaattca aaactgtggc
     3001 gatgcaggtg tagcggatga atgtcatcgc tcgcattgcg gaacttcaat cggtagcggc
     3061 ctccctggtg cagggtgacc atcggcttca tcgtctgcat cgaaaaagcg gcgccgttga
     3121 gcgtccactg attgaacccg ttgagtgcgg cgttgttctt gacgatggtc atctcgatcg
     3181 tctcgtccgg atgtgcaggt tcgccggtga tcttgccgaa acgcgcgtaa tcccagcgga
     3241 atggttttgg cttgatccac tgcgccttgc cggatcgccc cgcgtattcg accacgatgc
     3301 ccattccatg gcggcgatca tcatcgtcaa gatcaccgag tacccacacg ccggggtgat
     3361 tcatttcgac gatggcgcag atgcgttcgg cggtaccgag ccacagcacc ggcacctcgg
     3421 ttggagtcgg tacgggattt ccgtcgagcg caaccaccct gaaggaatgg cccggcagag
     3481 cgaggctgcg gatctcgccg gcgctggcgt tcaatacatg aaacaagaca cgctcgcctt
     3541 gtttgacgcg cacaggatcg ccggacccaa gcatcttgcc gttgatgccg aatcgcgcat
     3601 atccgacctc aaaccctttg gccttttctt ttgcctctgc gtcggcgcgt ttgccgattt
     3661 gctgtaattc tttgagcggc tcgccggcga gaaagtccat ggccatgtca ccgccgcggc
     3721 tgaaggaggg ttcaaattcc ttcatcacca ggaagatctc gcggtcatag gccccaggat
     3781 tgttcttggg gtcaatgtag acgggcccgg cttgacccgt gtaggtgccg cggttcaaat
     3841 cccctcccgc gaggacgtgc gtgtgataga accgaaaacc cgacggttcg ggtacgaatg
     3901 agacgcggcg catggcgtgc gcgggaacga agggagtgcc ttcttcggac gcgccgtcga
     3961 catcgctggc gatcctctga ccgtgccagt gcaccagctc aggggtatcg gtatcgttgg
     4021 agatgtcgat gacagtgcgt tggccctcct tgaagcgtag caacggcccc ggaaactggt
     4081 cgttgtagag cgtcgtcgag acgatgtgct cgggtgcgag atcgaccagc ccggtggcaa
     4141 tgcgcagcgc gtagtccgcc tttgtttccg ccgcggagga ggtttgggcc gtggactttg
     4201 accagggccc aaggctcgag ccgatcgcgg cgagacccga aagctttaga aattcacgtc
     4261 gcggaagtgt catcgatcgt cacctagaga cgcttgtcca cgtcctaccc tagcccatgc
     4321 agggtaaacc tgccaatcgg attgccgctt tgcgaccggg cgaggcgcgt gccgccgccg
     4381 gtggggtcgt tgtgtcaacg cgttgacaga actactttgc caattcgccc aacatgactc
     4441 ctgaagatgc aacgacactt attttaagga gaagatcatg aacaccagaa acctcgccaa
     4501 gaaaatcgcg gtggccaccg ttgccgcgct cggcgtcgcc tgcgccacgg gtgcccatgc
     4561 ggatgcagca ttggtcaacg cgccgacacg cacggtccac tatgcggatc tgaatctcaa
     4621 cacccaggcg ggcgctgcgc tgctctacaa gcgcatccga gtcgcggccg aagaagtctg
     4681 cggcgatgtg aattcgcgcc gcttggatga agcggcgatg gccaaggcgt gcgtggatcg
     4741 cgccgtctac ggcagcgttc agacggtgaa caacgtgaaa ttgaccaacg aatacaacgc
     4801 ccacacgggg gcgcagaaat cgatcaacgt cgccgccctg cgttgagcgg gccgcaccgc
     4861 gccgcattgg ccacccgcca atgcggcggt ccgtcgtgag ttaagtccta ctatgaatgg
     4921 aggagcgagc cggctgacgt ttggatcgct ttgcacgaag cttgctcccg tgtgcaagct
     4981 ggctcgtgct ccatttcatc agaggacgct acatgaataa cttacctgtg aacgctgttg
     5041 tactggcggt ggctttgata gccgtcgcgg tggttcttgt cgcgtggttt gccatacaaa
     5101 agcaacggac atcgagactc aagcagcgct tcggatcgga atacggacac gccgtaagtg
     5161 agtttggcag tcgatcaaag gccgaagcag aacttcgaaa acgcgaagtg cgcgtggagc
     5221 gcttgaacat cgcgccgctg acagcggcgg acgccacgcg attcgcccag gcctggagtg
     5281 cactgcaagg ccgcttcatc gacaacccca agggggtcgt cgtggaggcg gatctcctgg
     5341 tcagagaact gatggtcaag cgtggctacc caatgagcga tttcgagcgg cgcgcggccg
     5401 atatctctgt cgatcacccc gatgtggtga cgacctatcg cacggctcag gcgatcgccc
     5461 agcgtgatca acgcggcgaa gcggacaccg aggagttgcg caaggcggtg gtgcactacc
     5521 gcacgctgtt cgatgaactt ctcgaagtta agtcaacccc gtcgacggat ttggcgcctg
     5581 cccgcatccc ggtccactca tgagtgaccc aagcatgaga aaaccatcga caccggcagc
     5641 agatttcccc accgcagaaa gcgcggagca attgccctct gacttcgcga ttgcgccccg
     5701 cacaacaggt accgtcgacg atgacgacgc accggctgac gaacggcccg atgagaacga
     5761 agctcgggtg cgcgagaaaa gcgaaagcac tttagacggc ggtgtcgccg ctgaatgacg
     5821 attggtcggg cctaatcaat ccggcccgcg acccggctcc acaaagtcgt cgcgccgtgt
     5881 cgccgatttg ccggcatcga tcacgatcgt ctccgcgagt cgatcgtgcg cacattgccg
     5941 gttgcgctgc aggaaaaatt gcgcaaaacc aaagcccgct tcgagaaatg aagcgccata
     6001 acccagcgag cgctcgagcg cctgccaaaa agtgatgcgc tcgtgcgtga gcgagatgac
     6061 tttgatccgc atgaggcgtt tgccgatggt ctgcccgtta ccaatgtaaa gcgccaccca
     6121 gaaatacagg accagcacga tcacatccac cgcatgcggc agattgatca gcgtgtggcg
     6181 ttcccactgg tggggaaagt agttctccca caggaactcg gccggctttc tgagcattga
     6241 aacgaggaca aagtcgatgc cgaatccgat ggcgcggcga gtaaagggcg ccagctgcac
     6301 accgtcgatc gcatcttctc tggcggtttc gcgggcacga aaatacgtgg gcatggccca
     6361 gcatacctca tcgtctatgc agcaggtgcc agcccgcgcc gcgtcccgcg aacgaatgaa
     6421 aagctcttgg tcaattcggc gccgaagaga aatatttgag ccgagtaata ggcccacagg
     6481 agcagcacca ggagcgatcc agcggcgccg taaatgggcg cgaatgcgct tctgccgagg
     6541 tagtatccga tcgccagttt gccgatattg aacagggagg cggcgacgag ggcgccgacc
     6601 caaacatcgc gccactcgat tttgagctgc ggaatgtact tataaacaag cgcaaacaga
     6661 accgtggtaa tggcgagccc cacggtcaga tccaggagcg gcagcaaact tcgccattct
     6721 cccaagaatg aagcaatcca cgtgtcgagc gctgtgagta tggtgctaac caacagcgaa
     6781 accagcagca agaagccgac gccgaacacc agtcccagcg acagaaagcg ggcgcgcacg
     6841 acttgccaca atccgcccgc tctgctgcgc gcaggcgcgc cccaaatgcg gtcgagggaa
     6901 ttctgtagct cgacaaacac cgacgaggcg cccatcagca acaccacgac tccaatgagc
     6961 gtcgcgaggc ggctcttccc catgtgattg gcgctcagca gcaatgtctg aacggcggag
     7021 gcgccggcat cgccgaacag gacctgcatt tgcggcaaga gtcccgcctg taccgtgtcg
     7081 gtgccgatgg caagtccaac aacgccgatc gcgatgacga ggatcggcgc gatggagaac
     7141 accgtataga aggcgagcgc agcgcccatg gtcgacgcat cgtgacgaat ccaagcgagt
     7201 gccgcttgcg ccacgatgtc cttcgcgtct cgcagagtgg aaagtaaccg acgcacgaaa
     7261 tcaagtctca atgcccagct cgcgggacca tgagcggccg cgcgcgccgg caacgatgtt
     7321 atcggtctat tttgtcgatc ttcgatcctg ccaatgacag gtcgccgatc agtcccttct
     7381 cggcaaacac gaagccaacc accggcttct tgagagtgtc cgtatcgtag ttggcgccgc
     7441 tacctttgga aatcatcgcc acgcccgtat cggcgccgat ggaccaacct ttgctcgagg
     7501 tgaattgatt cagcgactcc tgcgtggtga atacgatgac ttcgctgcgc ttggacgcgc
     7561 cgagggtcaa tccgatcgat gccgaggaga cgctgtagta gccgaccgtc ttgccgtcga
     7621 ttcgcagggc tccctctccg tattcaccgc caacacctgc gcctgccttg gtgacgcggg
     7681 ggaataccaa ggtacccgcg gcggagtgca gcaactgccg attattggga ttcaaggcgt
     7741 cgaatgcctt gatggtttcg tggacgctga cgtcgagctc cggtttggtg tcggcgagca
     7801 ccgtgccgac ggcgagtgtc gaaagactaa cgccgagcgc ggcaacggat aagagcttat
     7861 tcatgatttc aatcctgtgt attcgtgcgc cgcatggcgc gcaagacaca tagtgcgcga
     7921 ggccacaaga gtggtctgtc tgttaccgca cgttccgatc ttgggcagtc gaatcgagac
     7981 taatcccgcg cggtcgcggg ctcggagcga tcggcaagtt catcaaccat ggcgatgatg
     8041 ccggcaagca ccgtgccgcc cacgcgcaga cagcagcctt cgatttgctg ccggttgaat
     8101 gatcttccta cggttcttcc cttggcatga atctcaactc tcgtgccccc cgggatatcg
     8161 ctggggcggc catggtcaat cgttccgaaa agaccgtgca gcaccaggcg gctcttgatc
     8221 tcatgtccga ttctcctcag cccggtatcg aacttttccg gtgactcgtc gatttccatg
     8281 gctctccttc gctctcgatc ttaacggatg cgcacgtatc ttatcagcgc gttagcgaac
     8341 acagcggctg atgagctaag tgcggtagcg caccgacgtt cccgccgcgc gccgatatat
     8401 tgtcaagcca ctcttttggg gcgaaccggc gcgcaatccg gacccgcgcc ctcggggcca
     8461 cacaacgctc ccaccggttt aatcaggaaa caacatgaac gcaagaataa tgatcggatt
     8521 ggcgctcgtg gtgggtctcg cagcgcacgc atcgactcct gaacgccgtg tcgaagtggt
     8581 gatcggcgac gacaatggtg ctgcgaccgc cgcgcgcagc taagttcgac ggtgttacga
     8641 ttcggctcgc cggtctcgcc ttgggacaac gatgagattc tccgcgctga actcttcagc
     8701 agcgagcggc tcgagcagca tgcggcgagc ctggcggccg cccagcaggt aacgcggcgg
     8761 cgctccgcga ggcgttccct caacgctcgc ctgaaggaga acgaatccgt tctgctcagc
     8821 gcctatcggg cgatcgcaac ggcggttggc gaaggccgct cgatcacgcc ttctgccgag
     8881 tggctcctgg acaattacca tctcgtcgag gagcagatcc gcgagattcg ggccgatcta
     8941 ccgccgggtt tctaccgaca attacccaag ctcgcggcag gacccttcgt cggctacccg
     9001 cgcgtgttcg ggctcgcctg ggcatacatc gctcacagcg acagccgctt cgatccggag
     9061 tcgctgcgct tatttgtgcg cgcctatcag cgcgtgcaac cgctgaccat cggtgagttg
     9121 tgggcggtcg ccatcacgct tcgcatcgtg ctggtcgaga acctgcgccg agccgcggtg
     9181 cgcatcgtca gcagccgtgc ggcgcgcgag gacgccgata ctgtggctga tcggctattg
     9241 ggcgtgaatg gctatgccgc ggagcccgac gcattggcgc gtcgcgtcgc aaccggcgcc
     9301 tcctttgcac cgccattcgt cgtgcaactg gtgctgcggc tgcgcgatca agaccccgac
     9361 acgacgccgg cccggcagtg gttggacacc ttcgtgaacg cacagggaag cacggcggac
     9421 gagatggtcc acggggagca tcagcggcag ggtgcatcga acgtgaccgt gcgcaacatc
     9481 atcacgagca tgaggctgct ctcggatgtg gactggcccg agcttttcga gagcgtgagt
     9541 ctcattgacg aattgctgcg ttcgaagagc ggtttcgcgg cgatggattt cgcaagccgc
     9601 gatctgtacc ggcgtgcgat cgagcaactg gcgcgcggat cgaagaagac tgaactggct
     9661 attgcctgcg cggccttggc ggcgggcgat gcggatccgg cgatcgatgg gttggatgtg
     9721 gagcgcaagc gtgacccggg ttaccacctg atcggcgccg gccggcgtgc gttcgaagcg
     9781 agcgttggct atcgaccttc cagatggaac tcggtcgccc gcttcaccac tcaaggcgcc
     9841 ggggaatata ttggcgcagt cgccctgatc gccgccatga ttctggctgt tcctctgctg
     9901 gaaatgcagg cgtggggagt cggcaacgcc tacctcgcac tcttggcggt cttgggcctg
     9961 gtgccgtcac tcgacgcagc gatggctttg gtgaaccgcg cggtcacgaa agggcggggc
    10021 gcgacgatcc ttccgggtct ggcactacgc gaaggcattc ccccgcacct gcgcacgatg
    10081 gtcgtggtcc ccacactgct caccacacgc gcggcgatcg aagaacacat cgagctcctc
    10141 gagatccact atctcgcctc gccggacggc gatcttttct tcgcactgct ctccgactgg
    10201 atggacgcgc cggcgcaaca caccgcagcg gacacccaac tgctggacat cgccatcgaa
    10261 ggcattgccc gcctgaatcg tcgacacggg gccgccccct caggcgatcg cttcgcgctg
    10321 tttcatcggc ggcgggtctg gagcgagggg cagaggcaat ggatgggttg ggagcgcaag
    10381 cgcggcaaac tgcacgaatt gaatcgactt ctgcgcggcg caccggatac gacattcgtc
    10441 gaactcggcg gccgtccgcc cgtcatccca gcgcaggttc ggtacgtcgt cacgctcgat
    10501 gccgatacgc ggctgcctcg agataccgtg cggcggctcg tcggtaaaat ggcgcatccg
    10561 ttgaatcagc cgcggttcga tgcgggcacc ggtcaagtcg tcgagggcta cgcggtgctc
    10621 cagccgcggg tcgcgccgtc attgccgacg gggcgcgagg gctcactttt ccagcgcgta
    10681 ttctccagca ccggcggaat cgatccctac gcggccgccg cctccgatgt gtaccaggat
    10741 ctcttcggtc tgggttccta tgccgggaaa ggcatttacg acatcgatgc tttcgaggca
    10801 gcgctcgccg gtcgggttcc cgaggggacg ctgctcagtc atgacctctt cgagggcacc
    10861 tttgcgcggg ctggactcgt ctccgacatc gaagtcgtcg aggaattccc gtcgcgctat
    10921 gacgtggcgg cggcgcggca acaccgttgg tcgcgtggcg attggcaact gctgccatgg
    10981 atactcgggc gcagcgatgc tgcgggcgac gaccgcggca gcggggccct gccgctcatc
    11041 ggcttgtgga agatgctgga caacctgcgg cgctccctgt cggccccggc ggccgtcgcg
    11101 gcgctcctcg ctggatggct gcttcccacg gcttctgccg ttctgtggag cgccttcatc
    11161 agtgcaacgg ttgcgttgcc gccgttgctc ccaatcttca ccgggatctt gccgagccgc
    11221 gccggcgtca cgctgcgcag ccacctgcgt gcgctgcgca gagacgtcgg gctcggtgcc
    11281 gctcagaccg cgttcatcct cacgtttctc gcccaccagg caagtttgat ggtggatgcg
    11341 atcggccgga ccctgtttcg cttgttgcgc cggcgcaatc tcctcgagtg ggtgaccgcg
    11401 gcccaggcga gactcaagtc gagcctcaca ctctcgagcg cttatgtcgg catggccggc
    11461 gcggtcgcga tcgcagccat cgacgccgtt atcgtcggcc acttccgagc ggataatgtc
    11521 tggctcgcgg cgcccttctt ggtgctctgg gcggcatcgc ccgcggtggc cgtatggatc
    11581 agcatgtctc cgctggtcgc gggccgccta accgtgtcgg cggcggatgc tcgcgcattg
    11641 agattggtgg cgcgtcgcac ctggcgatac ttcgagacct tcgtcacggc ggaggatcaa
    11701 aatttgccgc cggacaattt ccaggaggac ccgcggcccg tcatcgcgca tcgaacctca
    11761 ccgaccaaca tgggcctgta tctcttgtcc gcggtgagcg cccgcgattt tggctggtcg
    11821 ggaaccctcg atttcgtcga tcgtgtagag gcgaccttcg cgaccatgct tcgcctcgag
    11881 cgcttccacg gtcatttcta caactggtac gacacgcggg atctgcgccc gctggatccg
    11941 cgatacatct cgaccgtcga cagcggcaat cttgccggac acctgatcgc tctggcgaac
    12001 gcgtgccacg agcggatcga tctgccgctt cgctgcggtg aatcgcttgc cggcatccgc
    12061 gattctttga atctcgcgcg cgaagcgcgg ctggaactgc ccgacgaccg cagaatgcac
    12121 gccacgtcgc gagatcaaat cgatgccgcc atcgccgcgc tcgccgcggc gctcatgcag
    12181 gccgaggccg cgaccgtgga gatcgcgacc cagtggtcga ctttgatgcc gctcgccgtg
    12241 accctggtcg atatcgtgag cacgctcgcc ggcgagtgga gcggcgatgc ggccggggaa
    12301 ctgctctatt gggtcgaagc aaccaaacgc tcggtggaga gccggcgccg ggatgtcatg
    12361 cagacaccgg attcattgga cgatctgcag gcacgtctgg cggcaatcga agtgagcgca
    12421 cgcgccatgg cggacgccat gcagttcggc ttcctcttcg atccgcagcg ccggctcttg
    12481 tcgatcggct gtactgcgga tgcagcgctt gactccaatt gctatgactt actcgcctcc
    12541 gaggcacgcc tcgcgagctt catcgccatc gcaaagaacg acattccggc gcgccactgg
    12601 tttcgtctcg ggcgctcggt cacaccgatc ggcagagggg ctgcgctcat atcttggtcg
    12661 ggctccatgt tcgaatacct catgccgtcg ctcgtcatgc gggcaccggc cggaagtttg
    12721 ctcgagcaga ccaatcgcct ggtcgtgcag cggcagattc gttatggaat cgaactgcgt
    12781 atcccctggg gcatttccga atctgcctac aacgcccgcg atctcgagct gacttaccag
    12841 tattccaatt tcggtgttcc cgggcttggg ctcaaacgcg gcctcagcga gaatacggtg
    12901 atcgcgcctt atgccaccgc gcttgcggcc atggtcgatc cgcaacgtgc ggtgcgtaat
    12961 ttcgagcgac tcaacgtcat cggagcgcgt ggccaatacg gctttcacga agcactcgac
    13021 tatacgcgct cgcgtgttcc agacggcgcc gaggtggcga tcgtgcgtgc ctacatggcg
    13081 catcatcaag gcatgaccat cgcggcgatc gcgaacgtac tgcacgacgg gcagatgcgc
    13141 gcgcgatttc atgccgagcc cagcattcgg gcgaccgaat tgctgttgca ggagcgcgcg
    13201 ccacgcgatg tctcggtggt tcatccgcgg gcggaggaag tgaagacggc tgcaccgctc
    13261 ctcgatcctc gtgtccccga cgttcgtcgt ttgcacagcc cgttcgatgc cacgccgcaa
    13321 acacatctgc tgtcgaatgg ccgctactcg gtcatgatca cgggcgctgg ttctggctac
    13381 agccgctggc gggacattgc ggtgagtcgt tggcgggagg atgcgacgcg cgatgacacg
    13441 ggcagctata tctatctgcg cgatgtcgca agcggcgcgg tctggtcggc ggcctttcag
    13501 ccgagcggtg tcgagccgga tagctacctg gtcaccttta cggaggaccg tgcggaattc
    13561 atccgcacgg acgcgaccct cacgaccacc ctcgagatcg tggtatcgcc cgaggacgac
    13621 gccgaagtac ggcgcgtgtc gatcgccaat tccggttacc gcgaccggga gatcgaggtc
    13681 acgtcctatt tcgaactcgt actcgcgctg cctgcggccg acgcagcgca tcagaccttc
    13741 tcgaagctct tcgtgcaaac cgaatatatc gccaagcgca acacgctgct cgccacgcgc
    13801 cgcaagcgca cacccgacga gccggacatc tgggtgggtc atcatgcggt catcgagggt
    13861 agcgtgttgg gcgaagcgga aatcgagacg gatcgggcgc gttttctcgg ccgcggacag
    13921 gaagtcagat cgccgctcgc cgtgctcgac ggtcggcgct tgtccggttc cgtcggagcg
    13981 gtgctcgatc cggtgtttgc gcttcgctat cgactcaacg tggccgccgg tacaacggcg
    14041 cgcattgcct tctggaccgg cgtcgaggcc tcgcgcagcg cccttctcga cttgctcgat
    14101 aagcaccacg aggccaactc gttcacgcgc gcggccactc tcgcctggac gcaagcgcag
    14161 gtgcagttgc gccatcttgg cgttgattcg gccgaggcgg gccttttcca acgcctggcg
    14221 ggccacctgc tctatgccga tgcctcgatg cgctcgcctg cgaacacgat acgcgacggc
    14281 agcgggccgc cctcgggatt gtgggcgcag agaatctcgg gagatatccc gattgtgctg
    14341 ctgcgaatcg atgccgtcga ggacattgcc ctggcccgcc aagtggtgcg ggcacatgaa
    14401 tactggcgaa tgaagggctt ggccacggac ttggtgatcc tgaacgaacg cgcggcatcc
    14461 tacgtccagg atctgcagaa cgcgctcgag accttggtgc gtatgagtca gtcgcgaccg
    14521 cagcccggcg cgcacagcgt gcgcggcgcc gtgttcgtac ttcgcgccga tctcatctcg
    14581 gaacagacgc gcagcctcct gtcggcggtc gcgcgagttg tgctggtggc gcggcgcggc
    14641 ggccttgccg accaactcga ccgcgggcgc aagcccgtcg ggctcgcccc tgtggcgaga
    14701 aagcgtgtgc tgacgagcga agcgccgatg agcgctgatg cggggcatga tctcgaattc
    14761 ttcaatggcc tgggaggctt cggcgcgcaa ggccgggaat acgtgacgct tctcgggccg
    14821 ggacagtcga caccggcgcc gtggatcaac gtcatcgcca acccgcactt tggatttcag
    14881 gttgccgccg acggcagcgg attcacctgg gccctcaaca gccgcgaaca ccagcttacc
    14941 ccgtggtcga atgatcccgt cgcggatcgt cccggcgagg tgctgtatgt gcgtgatgag
    15001 gagagcggtg aggtgtgggg gccgacggcg gcgccgatcc gcgacccggg cgcaacctac
    15061 accgccacgc acggacaggg ctacagtaga ttcgagctga ctgcgcgcga catcgcgctc
    15121 gatctttgcc tgttcgtggc gatggacgat ccgatcaaga tctgccgcct gagaatacgc
    15181 aacacatcgc ggcgcagccg acggctctcg ctcaccgcgt acgtggaatg ggtactcggc
    15241 acatcgcgcg gctcgtcggc gccgttcatc gtgaccgaac tcgatacgga tacgggtgcc
    15301 ttgctcgccc gcaatccgtg ggacgccgcc ttcggctcac gagtcgcgtt tgcggatctc
    15361 gctggacggc aatcgcagtg gaccgcggac cgaggggagt tccttggacg ccatggtacg
    15421 ctcgacatgc ccgcggccat gagcactcgc gcgccgctcg ccggccgcgc cggcgccggc
    15481 ctcgatccgt gctgcgcact gcgcaccatg gtggacctgg agccgggcgc gaccagcgag
    15541 gtcgtcttct ttctcggcga agcagacaat gcgtcgcgcg cgcgctccct gctcacgcgt
    15601 taccgcggcg ccgatctcga cgccgtgttt cgcaaggtgt gcgagcattg ggacagcgtt
    15661 ctcggcgtgg tgcaggtcaa gacgcctgac cgctcgctcg atatcatgct caatcgctgg
    15721 atgctctatc aaacgctggc ctgccgcatc tgggcgcgca gcgcttttta tcaggcgagc
    15781 ggcgcctacg gctttcgcga tcagcttcag gatggaatgg cactcagcgt cgcgaggccg
    15841 gatctcacgc gggaacatct gcttcgcgcc ggcgcgcggc aattcggcgc aggagacgtg
    15901 cagcactggt ggctcccgcc ggtcgccgcg ggcgcgaccg gtcagggcgt tcgcacccgg
    15961 atcagcgacg atgtggtctg gctcgcctac tgcgcggtcc agtacatcga gtcaaccggc
    16021 gacgcagccg tactcgacga ggcgattccg tttctcgaag gccgctctct gcagcccgat
    16081 gaacacgacg ccttcttcat gcccgggata tcggaggaca cagcgacgct attcgaacat
    16141 tgcgctcggg cgctcgatca tgcgttaccg gtcggagagc acggtctgcc cttgattgga
    16201 actggcgact ggaacgacgg gatgaaccgg gtcggcgaga aaggcaaagg cgagagtgtc
    16261 tggctgggat ggtttctgaa tgcgacgctg tcatcgttcg cgcccctggc cgcggcgcgc
    16321 ggcgagcagg tgcgcgcgag caagtggcgc gcccatgcag cagcgctaca aaggtcgatc
    16381 gaacaggcgt gggacgggga ctggtaccgg cgcggctact tcgacgacgg gacagcgctt
    16441 ggatctgccg ccagcaccga gtgccgtatc gattcgatcg cccaatcatg gagcgtcatg
    16501 tcgggggccg gcgaccccgg ccgtcaatcg cgcgcgatgg ccaacgtgca aagccagctc
    16561 gtccgccccg acgtgggcct cgtgctgctg tttaccccgc cattcgatca caccgcgcat
    16621 gatccgggtt acatcaaggg ctacccgcct gggattcgag agaacggcgg acaatacacg
    16681 cacgctgccg cttggaccgt gatcgccgag gcgatgcggg gtcatggcgc ggaagccgcg
    16741 aaactatttt ccttcctcaa tcccatcaat cacacgagca gccgagccga cgtgcagcgc
    16801 tataaagtcg aaccctacgc ggtggccgcg gacgtctatt ccgtggcgcc gcacgtgggc
    16861 cgcgggggtt ggacctggta cacaggctca gcgggctgga tgtatcgcgc aggactcgag
    16921 tggattctgg ggtttcgggt gcgggcagga acacttctcg tgacgccgag cattcccgcg
    16981 gactggcccc gcatcgaaat cgtcttcagt cacgcctctg cgctctacga gatcctcatc
    17041 gaaaacccgc acggtgtcag ccgcggcgtc gcctacgtcg agttggacgg catacggctc
    17101 ggtgagcatc acatcgtatt ggcggacgat ggccgtcggc acgcggttcg ggtggtactc
    17161 ggcgaggcgt ccagcgtaac cacatacgcc tgagccgtca gcggaatttc tattggcaaa
    17221 atttgatact ccgtctacat aggctgctgt cccggctgct gcagattcac tatatgtgga
    17281 cattcacagt tcacagcagg cccgagcatc gaatgggaga gttgatcgca cggctcaccg
    17341 ggatcgacga gacgagtcgc acgcagcatg cttttctgca ggagggcgat cgatgcctgt
    17401 tcttcgctga atattttacg gggaaaggct atcaggctgg tgggatcaat cagctcatgt
    17461 tcaatttcaa gaccctgccc tcgattgcga gcgacaaccc gcttcgccgc ctccacaggg
    17521 agcgtgcgat atctacaatc gccacgggcc tgcgcgaggt tttgggccgc aagcaaatcg
    17581 aacgtctgac ctttgtgcca gtcccgccgt cgaaggcact cggccacagc gactacgacg
    17641 atcgactgac ccggactctg aagttgggtt gcaccgggtg cgatgccgac attcgcccgc
    17701 ttttgcgccg ctcctcgagc accgagcccg atcatcggag cggcgatcgg ctgacgcccg
    17761 acgagttgca cgcgttgctc gcactggact tcgcggccct cgccgcgaag ccgctgcgcg
    17821 aggtggtagt ggtcttcgac gatgtgatca ccacgggcaa atcgttcaaa tgttgcgagc
    17881 gccgattgcg cgaagtgatt ccggcgaccg tgcccatcat cggactgttc gtcgcgcggc
    17941 gaattcctca cactcccttc gatgcgctcg agcacgtctt cgcttcacgc gcgcatctcg
    18001 gaaactcgct ctaggcgatt gggggtccga tggagtattc tcaggccctc gtcgattgat
    18061 gcatcgctca aggaggaatc gccgatgaaa atgctcagcg tcctatcgaa atccgacgta
    18121 cgcggccttc tctcgagctt gctgctcggt ctcatggcgc tgccggccgc cgccgggtcg
    18181 gtgaccgatg cgaattacac catttccgcg agcgcctcag gcacacaatt gacgaccacc
    18241 gtgccgggtg cggcgacctt tgcagacagc agcaccttgg ccggtcaaca cccgcgtagg
    18301 cggggccatc gaggccaata gcatgtccgt tgacgttaaa taccgaacct ccgcgaccgc
    18361 caccgg
//
LOCUS       DASWZT010000328         4844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_89582, whole genome shotgun sequence.
ACCESSION   DASWZT010000328 DASWZT010000000
VERSION     DASWZT010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89582"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VGE92_09065"
     CDS             <1..434
                     /locus_tag="VGE92_09065"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEY1314016.1"
                     /translation="AARIALSVIFSVLALAMALWGARNFMVNYENFLSLLLCVMAPWT
                     AVNLVDFYLIQHGHYDVQSFFARDGGIYGRYNAVALSCYAVGILVQIPFLATDLYTGR
                     IAKALGGADISWLIALVVVSPLYFVWAGRAHRGMLANRQTE"
     gene            complement(364..2178)
                     /gene="atzF"
                     /locus_tag="VGE92_09070"
     CDS             complement(364..2178)
                     /gene="atzF"
                     /locus_tag="VGE92_09070"
                     /EC_number="3.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004039 - allophanate hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HEY1314017.1"
                     /translation="MALPDNLLIPWLRSGYLAGRFTPGDIVTQVMDRADRAAQRHIWI
                     NRLSRDQVMSYVQALQPAAPDDQPLYGIPFVIKDNIDLDGVPTTAGCPRFAYSPTRSA
                     TVVQRLLAAGAIPLGKANLDQFATGLVGVRSPYGACRNSFNEQFIAGGSSSGSAVAVA
                     CGLASFSLGTDTAGSGRVPAAFNNLIGLKPSLGRISTRGVVPACRSLDCVSIFALTCR
                     DAATVLDVAEGFDAEDPYSRRLGNTPIAGLRFGVPRPEQLQFFADADYARLFDAATTR
                     LEAGGGVRIEIDFAPFLDAARLLYGGPWIAERYAAVGKFMDDNPGALLPLTAHIIGAG
                     KVPSAAAAFEAQYELRVLKRAADAVWSQADYLVTPTAGTIYSLAAIEADPIKLNTALG
                     YYTNSMNLLDLAGVAVPAGFRTDGLPFGITLIGPASSERSLLALADIVHRSAVLTLGA
                     MPWPIGAIASEAPVLAAGYIELAVCGAHMQGLALNPQLRDRGAYFLKRTRTSPNYRLF
                     ALPGGPPHRPGLVRVPNEGASIQIEIWAVPVEQLGWLVAGIPAPLGIGKVEVENGGAV
                     SGFICEGYAVAGAADITRFGGWRAYLDERGPPIQSTTG"
     regulatory      2414..2513
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
     gene            2573..3298
                     /locus_tag="VGE92_09075"
     CDS             2573..3298
                     /locus_tag="VGE92_09075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea amidolyase associated protein UAAP1"
                     /protein_id="HEY1314018.1"
                     /translation="MIYETLIAGGKHWSLTVRAGLRLRIIDSDGGANVGMLLYNPQNP
                     LERYNAPDTLKCQHTFKLTKGHCLYSDMGRIFCSIIEDTAGWHDTVCGNANRQRVAER
                     WGEKSYQEHRNEWTQNGHDAFLVEGGKYGLSRRDLAANVNWFSKVSIDDDGRMQFDGS
                     RTRAGDYVDLRFEMNSLLLLHTCPHPLNPIAYYPRSPVVLQLSKADPVADDDVCRNAR
                     PENVRGFTNTELYQHCGDGAGRC"
     gene            3292..3948
                     /locus_tag="VGE92_09080"
     CDS             3292..3948
                     /locus_tag="VGE92_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005404253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea amidolyase associated protein UAAP2"
                     /protein_id="HEY1314019.1"
                     /translation="MLSTRASALEPAAALLRHVIPAGDYWIDTVRSGRTLRIVDVAGN
                     QAADTLFFNAADVHDRYSAVDTIREQGNVYLSCGSKLRSTAGNVLAEIVADTVGRHDT
                     LGGACASESNTVRYALDTRTMHACRDSYLLALAQHDHLGLNKRDLGHNINFFMNVPVT
                     RDGGLTFADGMSGPGQYVEMRAAMDIIVLISNCPQLNNPCNAYNPTPLEVLIWDADVS
                     "
     gene            3938..>4844
                     /locus_tag="VGE92_09085"
     CDS             3938..>4844
                     /locus_tag="VGE92_09085"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY1314020.1"
                     /translation="MFHKVLVANRGAIACRIIRTLRRLGVASVAVYSDADRNSLHVAQ
                     ADESVHLGAGPASESYLRQDRILAAARTTGAAAIHPGYGFLSENAEFAAACAAASIVF
                     IGPTPQQMRDFGLKHLARDLAARHGVPLLPGSALLSNAAEAASAAALIGYPVMLKSTA
                     GGGGIGMQLVRRESQLADAFHSVERLATKNFSRGGVFLEKYIERARHVEVQIFGNGSG
                     RVVALGERDCSAQRRNQKLIEETPAPGLDDGVRRRLLDCAIRLGEAVEYRSAGTIEFV
                     LDAVTQEFYFLEVNTRLQVEHGVTEE"
BASE COUNT          923 a         1475 c         1562 g          884 t
ORIGIN      
        1 cggcggcgcg aatcgcactg tcggtgattt tcagcgtgct ggcgctggcc atggcgcttt
       61 ggggcgccag gaatttcatg gtgaattacg aaaacttcct atcgcttctg ctgtgcgtca
      121 tggcgccctg gacggcggtc aatctggtcg atttctacct cattcaacac ggccactacg
      181 acgtgcagtc gttctttgcc cgcgatggcg gcatctacgg tcgttacaac gccgttgcgc
      241 tcagctgcta cgcagtcggt attctggttc agatcccgtt tctggcgacc gacctgtata
      301 ccgggcggat cgccaaggca ctgggcggag ccgacatctc gtggctcatc gccctcgtgg
      361 tggtcagccc gttgtacttt gtatgggcgg gccgcgctca tcgaggtatg ctcgccaacc
      421 gccaaaccga gtgatgtcgg cggcgcctgc tacggcatag ccctcgcaaa tgaatcccga
      481 gactgcgccg ccgttctcga cttcgacctt accgatcccc aacggcgcgg gaattcccgc
      541 gaccaaccag ccgagctgct cgacgggaac cgcccagatc tcgatttgta tcgaggcgcc
      601 ctcgttcggc acgcgcacca ggccaggacg atgcggcggt ccgcccggca gggcgaacaa
      661 tcggtaattc ggcgatgtgc gtgtacgctt gagaaagtat gcgcctcgat ctcgcagctg
      721 agggttcaat gccagaccct gcatgtgagc gccgcacacc gcgagctcga tgtagccggc
      781 ggcgagcacc ggcgcctcgc ttgcgatggc gccgatcggc catggcatcg cccccaaggt
      841 cagaaccgcg gatcgatgta cgatgtcggc gagcgcgagc aagctacgtt cgctcgacgc
      901 ggggccgatc aaggtgattc cgaacggcaa accgtcagtg cgaaacccgg ccggtaccgc
      961 aacgccggcg aggtccagca gattcatgga attcgtatag tatccgagcg ccgtgttgag
     1021 cttgatggga tcggcctcga tcgcggcaag cgaataaatg gtgccggcgg tgggcgtgac
     1081 caaataatcc gcctgcgacc acaccgcatc ggccgcccgc ttcagcactc gcaactcgta
     1141 ctgggcttcg aacgccgcgg cggcgctcgg aaccttgcct gcgccaatga tatgcgccgt
     1201 caacggcagc agagcgccgg gattgtcatc catgaacttg ccgaccgcgg cataacgttc
     1261 cgctatccac ggtccgccgt agagtagccg cgcggcatcg aggaagggcg cgaagtcgat
     1321 ctcgatgcgc acgccgccgc cggcttcgag acgcgtcgtg gcagcgtcga acaagcgtgc
     1381 atagtcggca tcggcgaaga attgtagctg ctcgggccgc ggcacgccga agcgcaaacc
     1441 ggcgatcggc gtattgccga gccggcgcga gtacggatcc tcggcgtcga agccctcggc
     1501 gacgtcgagc accgtggccg cgtcgcggca ggtgagggcg aaaatcgata cgcagtcgag
     1561 cgagcggcac gcgggcacga cgccgcgcgt actgatgcgg cccaaactcg gcttcaagcc
     1621 gatcaaattg ttgaacgctg cgggcacacg gccggaaccg gccgtgtcgg ttcccaacga
     1681 aaagctcgcg aggccgcagg cgaccgcgac cgccgatccg gagctcgagc cgccggcaat
     1741 gaattgctcg ttgaagctgt tgcggcacgc gccgtaaggc gagcggacac ccacgaggcc
     1801 ggtcgcgaat tggtcgaggt tggcttttcc caacggaatt gcgcccgccg ccagcagcct
     1861 ttgcaccacg gtcgccgatc gagtcggcga atacgcgaag cgcggacagc ccgcggtggt
     1921 cggtacgccg tcgagatcga tgttgtcctt gatgacgaag ggaattccat acagcggctg
     1981 atcgtcgggc gccgcgggct gcaacgcctg cacatagctc atcacttgat cgcgggacaa
     2041 gcgattgatc cagatgtggc gctgcgcggc gcgatccgcg cggtccatca cctgagtcac
     2101 gatgtcgccc ggggtgaagc gtccggcaag gtagccgctg cgaagccaag gaatcagcaa
     2161 attgtcgggt agcgccatgg cggtcaatat actttacgtc gccctggggt gcgcgcgagc
     2221 gacccagaag tgcgcgagtt tagggcgctc gagcgcgctg ctgcgccgtc ctggtgcgga
     2281 ctcgactcgc gtccgcgacg tcgcgcccgg catggcgcgc gccgggattt aagcaaatgc
     2341 cgtgtctgat gcgtaaatga aggcgaatct tgcgatgcgt cgcgaccggc atgacatttg
     2401 cagcacccca acatcaatgt gcgcctaggg ttccggcttg ggtttccaag cgcctggtcc
     2461 gagagacgca gtccttaccc aaggatacac ggcgggataa aagcccggga gaacaacgcc
     2521 gaggaggctt ataccctcgg gatgctcttg gccatgattt tggggaggct gcgtgatcta
     2581 cgaaactctc attgcaggcg gcaagcactg gtcgctgacg gtccgcgccg gcctgcggct
     2641 gcgcatcatc gacagtgacg gcggcgcaaa cgttggcatg ctgttgtaca acccgcaaaa
     2701 tcccctggag cgctataacg cgcccgacac cctgaagtgt cagcatacat tcaagcttac
     2761 caagggccat tgcctgtatt cggacatggg gcgaatcttc tgttcgatta tcgaagacac
     2821 ggcaggctgg cacgacacgg tgtgcggcaa tgcgaaccgg caacgggtgg ctgagcgctg
     2881 gggcgagaag agctatcaag agcaccgcaa cgagtggacc cagaacggcc atgacgcgtt
     2941 cctggtcgag ggcgggaaat atggactgag ccggcgcgat ctggccgcca acgtgaattg
     3001 gttcagcaag gtgtcgattg acgatgacgg tcgcatgcaa ttcgacggca gtcgcacgcg
     3061 ggccggtgac tacgtggatt tacgcttcga gatgaattcg ctgctgctgc tgcacacctg
     3121 cccgcatccg ttgaatccca tcgcgtacta tccgcgcagt ccggtggtct tgcagttgag
     3181 caaggcagac ccggtcgccg acgacgatgt atgccgtaac gcaaggccgg aaaatgttcg
     3241 cggcttcacc aacaccgagc tctaccagca ctgcggcgac ggagcaggac gatgttgagc
     3301 acgcgtgcga gcgcgctcga gccggcagcg gcactgctgc ggcacgtgat tccggccggc
     3361 gactattgga tcgacaccgt gaggagcggc agaacgctgc gtatcgtcga tgttgcggga
     3421 aatcaagctg ccgacacgct gttcttcaac gcagcggatg tccacgatcg ctacagcgcg
     3481 gtggatacca ttcgcgagca aggcaatgtc tacttgagct gtgggtcgaa gctcaggtcg
     3541 actgcgggca atgtgctggc ggagatcgtc gccgataccg tcggccgcca cgacacgctc
     3601 ggcggggcct gtgcgtcgga gtcgaatacc gtacggtacg cgcttgacac aagaaccatg
     3661 catgcctgtc gcgacagcta tttattggca ctcgcgcaac atgatcacct cggcctcaac
     3721 aagcgcgatc tcggccacaa catcaatttt ttcatgaacg tgccggtcac gcgcgacggc
     3781 ggtttgacct tcgccgacgg aatgagcggt cccgggcaat atgtcgaaat gcgcgctgcc
     3841 atggatatca tcgtgctcat ttccaattgt ccgcagctca ataatccctg caacgcctac
     3901 aatccgacgc cgctcgaggt actgatctgg gacgcggatg tttcataagg ttctggtcgc
     3961 gaatcgcggc gcgatcgcct gccgcatcat cagaacattg cggcgcttag gtgttgcttc
     4021 ggtcgccgtg tattcggatg cggaccgcaa ttcgctgcac gtggcgcagg ctgacgagtc
     4081 cgtgcacttg ggtgccggac ctgcgtcgga gtcgtatttg cggcaggaca gaatactggc
     4141 cgccgcccgg accaccggcg ccgcggccat tcatcctggc tatgggttcc tgagcgaaaa
     4201 tgccgagttc gcggcggcgt gcgcggcggc gtcgatcgta ttcatcggac ctacgcctca
     4261 gcagatgcgt gattttggtt tgaagcattt ggcgcgagac ctggccgccc gccacggtgt
     4321 gccgctcttg cccggcagcg ccctgttgtc gaacgccgcc gaggccgcaa gcgcagccgc
     4381 ccttatcggc tatcccgtca tgctgaagag taccgccggg ggcggcggca tcggcatgca
     4441 actggtgcgc cgggaatcgc aactggccga cgcctttcac tcggtggaac gactggcgac
     4501 caagaatttc agccgcggcg gcgtgttcct ggaaaagtac atcgagcgcg cgcggcacgt
     4561 cgaagtgcag atcttcggca atggcagcgg gcgcgtggtg gcactgggtg aacgcgactg
     4621 ctcggcgcag cgccgcaacc agaagctgat cgaggagaca cccgcgccgg gccttgacga
     4681 tggcgtgcgc aggcgtcttc tcgactgtgc aattcgtctg ggcgaggcgg ttgagtatcg
     4741 atcggcgggc acgatcgaat ttgtcctcga tgccgttacc caggagttct atttcctgga
     4801 agtcaacaca cgtctgcagg tggagcacgg agtcaccgag gagg
//
LOCUS       DASWZT010000329         1554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_94631, whole genome shotgun sequence.
ACCESSION   DASWZT010000329 DASWZT010000000
VERSION     DASWZT010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94631"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(322..>1554)
                     /locus_tag="VGE92_09090"
     CDS             complement(322..>1554)
                     /locus_tag="VGE92_09090"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1314021.1"
                     /translation="DNLRPTESSRATPAVTVPSADLSGNYSDVASQVVHDGSIDAGRV
                     GEFENEARGGNPSAVGKVIAAHHAAAQFLIAKGDLDAAAEHYHRASILAPNNVPVLLN
                     LAVLDLRRSQYKAAMDPLERARRVEPDSADVAKLMGWAYYGANNIDRAVAEWKRALQL
                     HPSDGEVQSALAKAVRDQQEEASYREGETAHFTVKYSGAANPDLAREILRTLEEHFRS
                     IEGQLDYTPPESIGVILYTQQAFADITRAPGWAGAINDGRIRVPTQGLTSMTPELSRV
                     LKHELTHSFVGQKTRGRCPTWLQEGLAQWMEGRRSGDSAGILLQVYDHEMAPSLATME
                     GSWMSMSGDSASYAYAWALAAVESIINSGGMSDIERLLGQIAAAPTTEEALRDSLRTS
                     YSDLSQQTATYLRREYSR"
BASE COUNT          276 a          537 c          439 g          302 t
ORIGIN      
        1 ggatccattt gccacgctca cgaagcacag tccgagaacg gcggctgcgg cgagaatatc
       61 ccgattgaag tccatggctg aatccttccc aggcgtggaa agcgaaacac gcgggcgaaa
      121 gctggcacct atccgatgag atcgggccgc acttggtgtc aggatcgtaa gccgctccac
      181 atactctttg gttcatcccg gctcatcgat tcgccaactg tccgagcggc agtccccgtc
      241 cccgcgcacg ccgcgcgaga aggggattgg ccacaccccg gcaccggctc ggccacctgt
      301 gggattttgt agagggaaaa attaacgcga gtattcgcgg cgaaggtaag tcgccgtctg
      361 ttgcgatagg tcggaatacg aagtgcgcaa tgaatcgcgt agggcttcct cggtcgttgg
      421 cgccgccgct atctgcccca gcaaccgctc gatgtcgctc atcccgccgc tattgatgat
      481 cgactcgacc gccgccagcg cccatgcgta cgcgtacgac gccgaatccc ccgacatgct
      541 catccacgag ccttccatcg tggccaacga tggcgccatc tcgtggtcgt acacctgcag
      601 caatatgccc gccgaatcgc cgctccgccg cccttccatc cactgcgcca gcccttcctg
      661 cagccacgtc gggcagcgcc cgcgcgtttt ttgcccgacg aagctgtgcg tcaattcgtg
      721 cttcaacacg cggctcaatt cgggcgtcat cgacgtcagc ccctgcgtcg gcacgcggat
      781 gcgcccgtca ttgatcgcac ccgcccagcc cggcgcgcgc gtgatgtcgg cgaatgcttg
      841 ctgcgtgtaa aggatcacgc cgatggattc cggcggcgta tagtcgagct gcccttcgat
      901 ggagcggaag tgttcttcaa gcgtgcgcaa aatttcgcgc gccagatccg gattggccgc
      961 gcctgaatac ttcaccgtaa aatgcgcggt ctcgccttcg cggtaactcg cctcttcctg
     1021 ctggtcgcgc accgctttag cgagcgcgct ttgcacctcg ccgtccgacg gatgcaattg
     1081 cagcgcccgc ttccactcgg ccaccgcgcg atcgatatta ttggcgccgt agtacgccca
     1141 gcccatcagc ttggccacat ccgccgaatc gggctcgacg cgccgcgccc gctcgagcgg
     1201 atccatcgcc gctttgtact ggctccgccg caaatccagc accgccagat tcagcaatac
     1261 cggcacattg ttcggagcca aaatcgatgc gcgatggtag tgctcggcgg ccgcgtccag
     1321 gtcgcctttt gcaatcagga attgcgcggc agcatggtgc gcggcgatta ctttaccgac
     1381 ggcgctcgga ttgcctccgc gcgcctcatt ctcaaattcc ccaacgcggc cggcgtcgat
     1441 cgacccgtca tgtaccacct ggctcgccac atccgagtag ttccccgaaa gatcggcgct
     1501 cggtacggtg accgccggcg tcgcacggct cgattccgtc ggcctcaaat tatc
//
LOCUS       DASWZT010000330         3561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_60668, whole genome shotgun sequence.
ACCESSION   DASWZT010000330 DASWZT010000000
VERSION     DASWZT010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60668"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1765
                     /locus_tag="VGE92_09095"
     CDS             <1..1765
                     /locus_tag="VGE92_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1314022.1"
                     /translation="DIAAVRLVGWDVHNAGYISNVAKSYTFPTSGIVFSNAALVAKDY
                     NTTETKGGRSALRVILGENWTVTPTVMGQVTTTNGPFAYNPAAGDLNTWQFFPDPTRD
                     SWVQSSLTVEGKISDFDVVYAGAYLTRNTHEQSDYTDYSLFYDRTYGSGAYFLDNAGK
                     LINPAQDIQGRDHYTKTSHELRMTSPKDYRLRVTAGLFLQRQVHEILQDYVVDNDNDP
                     LGSVAPNNISVPGWPGTLWLTDQERVDRDSAEFGEASFDFNQYLTANGGIRFYKYDNT
                     LQGFYGFNSTFSTHEGIATCFTPFVPFHGAPCQDLNGRTSSSGNTPKLNLTYKIDKDH
                     MVYATWSKGFRPGGVNRNGGGTLPPYQPDYLTNYELGWKTTWLDHRLRFNGAIFDERW
                     KNFQFAYLGANALTIIANAGNAEIKGIESELEFAVTEGLTISGGFSWLDAKLTQAYCD
                     NPAMCLTPGYEDYAPAGTRLPVTPKFKGDITGRYTFPVGSYKGNVQVSGVYVGSRTSD
                     LRVVAANALGDEPAYAVADISTGLEVNKLTFELYVNNVADKRAVLDRFAECDAESCGQ
                     IAVYNVPNQPRTVGFKFGEKF"
     gene            1861..3123
                     /locus_tag="VGE92_09100"
     CDS             1861..3123
                     /locus_tag="VGE92_09100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY1314023.1"
                     /translation="MPNLYQDTAEPATATPPLDGDARADVLVIGGGITGLSTALHLAE
                     RNTAVVLLEAEEPGWGASGRNGGQVNPGLKHDPDRVERDHGPDLGGRMNALAGGAPGL
                     VFDLIARHAIRCEARRNGTLRAATRAQHAAQVRATAQQLTRRGAPIELLEGAALERAT
                     GTTRYALAMLDRRGGDLNPLSYARGLARAALRAGAVVHGGTRALSMKRAGAVWQVLTA
                     RGTVSAARVVLATNGYTDSLWPGLEQTIVPLFGAIAASAVLPEEIARGIMPSRAVLYE
                     SGAITVYYRVDSGQRLLIGGRGPMREVTATAAIAHLLAYARTLWPALAQTAWTHAWGG
                     RLAMTPDHYLHVHEPADGILICLGYNGRGVAMSTAMGAQIAERLTNRSASFDMPITRL
                     APIALHACWPLAVRGAIALGRIRDFLGL"
     gene            complement(3137..>3561)
                     /locus_tag="VGE92_09105"
     CDS             complement(3137..>3561)
                     /locus_tag="VGE92_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314024.1"
                     /translation="IGPGGRRLLASLVVSIAFLSAQHHVYALIAVPQGNEWQLIMDGA
                     KHVHLNGVHPPRIFAIASTPADISTATIYHDEFGSLSTNSEWVPKEMFKRAMHDLHPE
                     MANLDRRYEFASGPKLPTDQHFDVIIDLHRLRKAYTDN"
BASE COUNT          651 a         1136 c         1148 g          626 t
ORIGIN      
        1 cgacatcgcg gcggttcgct tggtgggttg ggacgtgcac aacgccggct atatcagcaa
       61 cgtggccaag tcctacacgt tcccgacctc gggaatagtc ttcagcaacg cagcgctggt
      121 tgccaaggat tacaatacca ccgaaaccaa gggcggccgc tcggcgctgc gggtcatctt
      181 gggcgagaat tggaccgtca cgccgacggt catgggtcag gtcacgacca ccaatggtcc
      241 gttcgcctac aatcccgcgg cgggcgacct caacacctgg caattcttcc cggatccgac
      301 ccgcgactcg tgggtgcagt catccctgac ggtcgagggc aagatcagcg atttcgatgt
      361 ggtctacgcg ggcgcgtacc tgacgcgcaa cacccacgag caatcggact ataccgatta
      421 ctcgttgttc tacgaccgga cttacggttc cggtgcatac tttttggaca acgccggcaa
      481 gctcatcaat ccggcgcagg acattcaggg ccgcgaccac tacaccaaga ccagccatga
      541 gctgcgcatg acgtcgccca aggattatcg gttgcgcgtg accgcgggct tgttcttgca
      601 gcgccaagtg cacgagatct tgcaggatta cgtcgtcgac aacgacaacg atccgctggg
      661 ctcggtggcg ccgaacaata tctcggtacc gggttggccg ggaactctgt ggctcaccga
      721 ccaggagcgc gtcgaccgcg actccgcgga gttcggcgag gcgtccttcg acttcaacca
      781 gtacctgacc gccaatggcg gcatacgctt ctacaagtac gacaatacgc tgcaaggttt
      841 ctacggattc aattcgacgt tcagcaccca tgagggcatt gccacctgct tcacgccgtt
      901 cgtgcccttc cacggcgcgc cgtgccagga cctcaacggc cgcacctcga gttccggcaa
      961 tacgcctaaa ttgaatttga cctacaagat cgacaaagat cacatggtgt atgcgacctg
     1021 gtccaagggc tttcgtcccg gcggcgtgaa ccgcaatggc ggcggtacgc tgccgccgta
     1081 tcaacccgac tatctcacca actatgagtt gggttggaag accacctggc tcgatcaccg
     1141 cttacgcttc aacggtgcga tcttcgatga gcgctggaag aatttccagt tcgcgtatct
     1201 cggcgcgaat gcactcacga tcatcgccaa cgccggcaac gccgagatca aaggcatcga
     1261 atcggaactg gagttcgccg tcacggaagg gctcaccatc tcgggcggct tctcctggct
     1321 cgacgcgaag ctcacgcagg cgtactgcga caatccggcg atgtgtctca cgccgggcta
     1381 tgaggattac gcgcccgcgg gcacgcgctt gccggtcacg ccgaagttca aaggcgacat
     1441 caccgggcgc tacaccttcc cggtcggcag ctacaagggc aacgtccagg tctcgggcgt
     1501 ctatgtggga tcgcgcacct cggacttgcg cgtggtcgcg gcgaatgcgc tcggcgacga
     1561 gccggcctac gccgtcgccg acatttccac cggactcgag gtcaacaagc tgaccttcga
     1621 gctgtacgtc aacaatgtcg ccgacaagcg tgcggtactc gatcgctttg ccgagtgcga
     1681 tgcggagagc tgcgggcaga tcgcggtgta caacgtgccg aatcagccgc gcaccgtcgg
     1741 tttcaagttc ggggagaaat tctaagagcg gccgagcacg gcgccgcgca cggcgcagat
     1801 ccactgcagg ggttcggcta tcatcgctgg cggccagctc agcgcgcgac gacgattcaa
     1861 atgccgaact tgtatcagga caccgccgaa cccgcgacag cgacgccgcc gctcgacggc
     1921 gacgcgcgtg ccgatgtgct ggtgattggc ggcggcatca ccggcttgtc gaccgcgctg
     1981 catctcgccg aacgaaacac ggcagtcgtg ctgctcgagg ccgaagagcc gggttggggc
     2041 gcctcgggtc gcaatggcgg acaggtcaac ccgggtttga agcacgatcc ggatcgtgtg
     2101 gagcgcgacc atggcccgga tttaggaggg cgcatgaatg cccttgcggg cggcgcgccg
     2161 gggttggtct tcgatctcat cgccaggcat gccatccgct gcgaggcgcg ccgcaacggc
     2221 acgctgcgcg cggcgacacg ggcgcagcac gccgcgcagg tgcgcgccac cgcgcagcag
     2281 ctgaccagac gcggcgctcc gatcgagctg ctggaaggcg cggccctcga gcgggcgacc
     2341 ggtacgaccc gctacgcgct cgcaatgctg gatcggcgcg gcggagattt gaatccgttg
     2401 agctatgcgc gcggcctggc gcgcgccgca ttgcgcgcgg gcgccgtggt gcacggcggc
     2461 acgcgggcgc tttcgatgaa gagggcgggg gccgtgtggc aggtgctgac ggcgcgaggc
     2521 acggtgtccg cggcgcgcgt ggtgctcgcc accaacggct acacggacag cctctggccg
     2581 gggctcgagc aaacgatcgt gccgctgttc ggcgcgattg ccgcgagcgc cgttctgccc
     2641 gaggagatcg cgcgcggcat catgccgagc cgcgccgtgc tctacgaaag cggcgcgatc
     2701 acggtctatt accgggtcga cagcgggcag cggctattga tcggcggccg cggcccgatg
     2761 cgcgaagtaa cggccaccgc ggcgattgcg catttgctgg cctatgcacg caccctgtgg
     2821 ccggcgctgg cgcaaaccgc ctggacgcat gcctggggcg ggcgcttggc gatgacgccg
     2881 gatcattacc tgcacgtcca tgagccggcc gatgggatct tgatctgctt gggctacaac
     2941 ggccgcgggg ttgcgatgag cacggcgatg ggcgcgcaga ttgcggagcg actgacgaat
     3001 cgctcggcga gtttcgacat gccgatcacc cgcttggctc caatcgccct gcacgcgtgc
     3061 tggccgttgg ccgtgcgcgg cgcgattgcg ctcgggcgca tccgcgattt tctcggcctg
     3121 taagcgcgcg cagcgctcaa ttgtcggtgt aggcctttcg cagccgatgc aagtcgatga
     3181 tcacatcgaa gtgctggtcc gtcggcagct tcggacccga ggcaaattcg tagcggcggt
     3241 cgaggttcgc catctcggga tgcaaatcgt gcatcgcgcg tttgaacatc tccttcggca
     3301 cccattcgga gttcgtcgac agcgacccga actcatcgtg atagatcgtc gccgtcgaaa
     3361 tatccgcggg agtcgaggcg attgcaaaaa tacgcggcgg gtgcacgccg ttcaggtgca
     3421 catgtttcgc cccgtccatg atcaactgcc actcattgcc ctggggtacg gcgatcagcg
     3481 catagacatg gtgttgcgcg ctcaagaacg cgatcgatac gacgaggctc gccaacagcc
     3541 gccggccgcc cggtccgatg c
//
LOCUS       DASWZT010000331         2549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_7419, whole genome shotgun sequence.
ACCESSION   DASWZT010000331 DASWZT010000000
VERSION     DASWZT010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7419"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..592)
                     /gene="phaR"
                     /locus_tag="VGE92_09110"
     CDS             complement(89..592)
                     /gene="phaR"
                     /locus_tag="VGE92_09110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEY1314025.1"
                     /translation="MSNARVIKKYANRRLYDSTGSRHVTLEDIRKMIVAGDKVKIIDD
                     KSGEDLTRAVLLQVISEQEQFGTPVLSTELLESIIRFYGNPVQEMLTRYMEQSIGSFA
                     RQQQTMRAEMAKVLEGPMAPMTEFARQNMEQWSKIQASMLAAFAPSKPPAAAADDTPP
                     EQPKKSD"
     gene            737..1444
                     /locus_tag="VGE92_09115"
     CDS             737..1444
                     /locus_tag="VGE92_09115"
                     /inference="COORDINATES: protein motif:HMM:NF021246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(R)-hydroxyalkanoic acid synthase subunit
                     PhaE"
                     /protein_id="HEY1314026.1"
                     /translation="MNDSSAASGAQPAFLRAWDDYAAACQTLFERFSDPAAGDPAANR
                     GAAVPLAFFGAWQDFAKNLGMPSDPGGAAKPKPEELFARFLPALGHTREYQEIAQRML
                     DLGAQFQRRSAEFMQYGVDIGQRAMEALQKQSANDASVFSSTAAYYDAWIDNAEQSYA
                     QTAHSEPFAVLLAQLCNIMSAFKVERGKLLEAFARQLDLPSRAEVDSLHRQVRELRVA
                     ARPLKSRTRKQRKSSDK"
     gene            1441..2511
                     /gene="phaC"
                     /locus_tag="VGE92_09120"
     CDS             1441..2511
                     /gene="phaC"
                     /locus_tag="VGE92_09120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HEY1314027.1"
                     /translation="MTASSDLFARFLQDTGALREQSQAALHALRDLGELNVGSSEKTR
                     VYAEHKVQLYRYAPLARSAKLPPLLICYAMVNRPYMLDLQPDRSLIRELLFLGIDVYL
                     IDWGYPDGADRFTPLEEYICGYLGRCVEFILKQNRIRALNLLGVCQGGTMSLCYSALE
                     PERIANLITMVSPVDFKTPENLLSKWAQHIDVDLLTQSGNVSGEFLNLVYLALMPFRL
                     TQQKYVNLLQMQGDRAQLENFMRMEKWIFDSPDQPARAFREFLQWFFQENRLVRDALT
                     LDSRPVRLKNVTCPVLNIYAANDHLVPPSASIPLGAHVGSEDYEALRVDVGHIGMYVS
                     GKARKSVPANIARWLGERKPVK"
BASE COUNT          503 a          752 c          766 g          528 t
ORIGIN      
        1 gcgaccgatg ccgcgcgcgg cgccggtaat gacgatggtt ttgtctttga tttgcataga
       61 gatcatcagc gcttgaagcg gtgctccgtt aatcactttt cttgggttgt tcgggcggcg
      121 tgtcatcggc ggccgccgcg ggcggcttcg atggcgcaaa cgcggccagc atggaggctt
      181 gaatcttcga ccactgttcc atgttttggc gggcgaattc cgtcatgggg gccatcggac
      241 cttccagcac tttggccatt tccgcacgca tggtctgttg ctgacgagcg aacgagccga
      301 tgctctgttc catatagcgg gtcaacattt cctggaccgg gttgccatag aatcggatga
      361 tcgattcgag caactccgtg ctcaaaacgg gcgtaccgaa ctgttcctgc tcggaaatca
      421 cctgcagcag caccgcacgc gtcaaatctt caccggattt gtcgtcgatg atctttactt
      481 tgtcacccgc gacgatcatt ttgcggatgt cttccaacgt gacatggcgg ctgcccgtcg
      541 aatcgtaaag ccgacggttc gcgtacttct tgatgactcg tgcattggac ataatgtagt
      601 tcagtaaaaa tcggccagcg cagcgtctcc cagggcgcga agcataacgc ttttcagtat
      661 gcgttgcaca agtgatataa ggtaggaatt tgcccacaaa tcttcaaata atcttgcgcc
      721 gcaaaggggg tcgggggtga acgattcaag cgccgcctcg ggagcgcagc ccgcgtttct
      781 gcgggcgtgg gacgactacg ccgcggcgtg ccagaccttg ttcgagcgct tcagcgatcc
      841 ggcggccggc gatccggcgg ccaaccgggg tgcggcggtg ccgctggcct tcttcggagc
      901 ctggcaggat ttcgccaaga acttaggaat gccgtccgac ccgggcgggg cagcgaagcc
      961 caagccggag gagctgttcg cgaggtttct accggcgctc ggccacacgc gcgagtatca
     1021 ggagatagcg cagcgcatgc tcgacctcgg cgcacaattt caacgacgct ccgccgagtt
     1081 catgcaatac ggcgtcgaca tcgggcagcg cgcgatggag gccctgcaga agcagagtgc
     1141 caacgacgcg agcgtgttca gttcaacggc agcctattac gatgcctgga tcgacaatgc
     1201 cgaacagtcc tatgcgcaga cggcgcacag cgagccgttt gcggtgctgt tggcgcagct
     1261 gtgcaatatc atgagtgcgt tcaaggtcga gcgcggcaaa ttgctcgaag cgttcgcgcg
     1321 acaattggac ttgccgagcc gcgccgaagt cgactccctg caccgtcagg tacgcgagct
     1381 cagggtcgcg gcccggcccc tcaaatcacg aacgcgcaaa cagcgcaagt cttcggataa
     1441 atgaccgcca gctcggacct attcgcgagg ttcctgcagg acaccggcgc gctgcgcgaa
     1501 caaagtcagg cggccttgca cgcgctgcgc gacctcggcg agctcaacgt cggctcatcg
     1561 gagaagaccc gcgtctacgc cgaacacaag gtgcagttgt atcgctatgc gccgcttgca
     1621 cgctcggcca agctgccgcc gttgctgatt tgctatgcca tggtcaatcg tccgtacatg
     1681 ctcgacttgc agccggaccg gtcgctgatc cgcgaactgt tgtttctcgg gatcgatgtg
     1741 tacttgatcg actggggcta tccggatggc gccgatcgct tcacgccgct cgaggaatac
     1801 atctgcggtt atctggggcg ctgcgtcgag ttcattctca agcagaaccg catccgcgcc
     1861 ctgaatttgt tgggtgtctg ccagggcggc acgatgagct tgtgctactc ggcgctcgaa
     1921 cccgagcgga tagcgaatct cattaccatg gtgtcgccgg ttgatttcaa gacaccggag
     1981 aatttactct cgaagtgggc ccagcacatc gacgtcgatc tgttgacgca atcgggaaat
     2041 gtttccggcg aattcctgaa cttggtttat ctcgctctga tgccgttccg cttgacccag
     2101 cagaagtacg tcaatcttct gcagatgcag ggcgatcgcg cgcaactcga gaatttcatg
     2161 cgcatggaga aatggatttt cgacagcccc gatcagcccg ccagggcatt tcgcgaattt
     2221 ctgcagtggt ttttccagga gaaccgcctg gtccgcgatg cgctaaccct cgattcaagg
     2281 ccggtgcggc tcaagaatgt cacgtgcccg gtgctgaata tctatgccgc gaacgatcat
     2341 ctggttccgc cgagcgcgag cattccgctc ggcgcgcatg tcggcagcga agactatgaa
     2401 gcgctgcgag tcgacgtcgg ccacataggc atgtatgtca gcggcaaggc ccgcaaatcc
     2461 gtgcccgcaa acatcgcccg ctggctcggc gaacgtaaac ccgtcaagta gcgccggcca
     2521 ccgctgcagc ggcggaaacc aacggcagg
//
LOCUS       DASWZT010000332         2940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_2634, whole genome shotgun sequence.
ACCESSION   DASWZT010000332 DASWZT010000000
VERSION     DASWZT010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2634"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..>2940)
                     /locus_tag="VGE92_09125"
     CDS             complement(215..>2940)
                     /locus_tag="VGE92_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HEY1314028.1"
                     /translation="LAARLEIRAAVFAEYAGRPQTMTDHARILAATLGLRPPSAADLP
                     FMIEAAAQAAWSTDRGQPIAAGVVSALRTEKIILPAPAVVERAAIAGRARARKRASDA
                     LLAGVSSEQLIELDRLLTVDPTTGATPLAWLRNTPVSPKADNIRELLDRLRSVRDIGL
                     PADAAAQVHEDRFQQLVREGRISDAHQISRYAAHRRRAILVATCIDLETGITDAVLDM
                     ADKLIGGLFARARKAKERRYVASTRDVGRLMRLFHDTIAALDEARKSKRDGFAVVDEA
                     VGWAKLLRVQAEVTKLANLADEDPLRGAADRYLTLRKFAPELIEALEFKAARSNDPTL
                     SALRLLQDLNRTGKRDVPADAPMPFRKDWKLLVSEEGRTNRRLYETAVFATLRDRLRS
                     GDVWVERSSNYRRFDSYLLPPSAVPAIAADMGLPATADEWLAARGAELDGRLKRFARQ
                     LRRGELEGVELRDGRLHVSPVKAATPPEADALADRIDAMLPRARITEVMHEVSRATGF
                     TAAFTNLRTGECCENENALLAAILADASNLGLTRMAAASQGVTRDQLLWTADAYIRPE
                     TYKEALARIINAHHALPIAAIWGDGSTSSSDGQFFRSAKRGDAAGEINARYGHDPGLS
                     FYTHLSDQHGPYHIKVMSATSHEAPYVLDGLVHHGTQLNIGTHYTDTGGASDHVFILC
                     PMLGFRYCPRLRDLPDRKLACIEPASNYKDLQPLLGQRVKVDVVREHWDEAIRLFASL
                     KAGTVAPSTMLKKLAAYRRQNQLDLALQEMGRIERTLFMLDWLESPELRRRCHAGLNK
                     SEQRHFLAQVICTFKQGRIADRGMEAQQFRASGLNLVIAAIVYWNSTYIADAITHLRA
                     TGEVVPAALLSHTTPLSWEHIGLSGDFLWDHAAATAARRRPLNLSRARAAA"
BASE COUNT          526 a          897 c          976 g          541 t
ORIGIN      
        1 ggtaagggga ggccatgcgc tgagggacgc aagtggtgat cgacagttcg cactcaagat
       61 cgcatcaagg ctcgatcttc catcgaaccc tcccccggga atcctttggt caccctacgt
      121 aagcggtttc gtagcggaag atcgctacgt gagggcatag cgtcggttgt tagataaaca
      181 acgctaaggt cgaacggacc aagaacgaga ctgatcacgc ggcggctctg gcgcggctga
      241 ggttgagagg gcgccgcctg gcagcggtgg cggcagcgtg gtcccagagg aaatcgcccg
      301 acaggccgat atgctcccag ctcagcggtg ttgtatggct gagcaacgcc gcgggcacga
      361 cctcgcctgt ggcgcgcagg tgcgtaatag catcggcgat gtaggtcgaa ttccaataga
      421 caatggcggc gatgaccagg ttcagccccg aggcccggaa ttgctgcgcc tccatgccgc
      481 ggtcggcgat gcggccctgc ttgaaggtgc agatgacctg ggcaaggaag tggcgttgtt
      541 cgctcttgtt cagcccagcg tggcagcgcc ggcgcagctc cgggctttcc agccaatcaa
      601 gcatgaacag tgtgcgctcg atccgtccca tttcttgcag cgccaagtcg agttggtttt
      661 gtcggcgata agccgcgagc ttcttcaaca tcgtggacgg tgccacggtg ccggccttca
      721 gcgacgcgaa cagacggatc gcctcgtccc aatgctcgcg gacgacatcg accttgaccc
      781 gttgccccag cagcggctgc agatccttat agtttgacgc cggctcgatg caggccagct
      841 tccgatccgg caggtcacgc agccgtgggc agtagcggaa cccaagcatg gggcagagaa
      901 tgaagacatg gtccgaagcg ccgccggtat cggtatagtg ggtgccgatg ttcagctgcg
      961 taccgtgatg caccaggcca tcgagcacgt acggcgcttc gtggctcgtc gccgacatca
     1021 ccttgatatg gtaggggccg tgctgatcgg agagatgcgt gtaaaagcta aggccaggat
     1081 catggccgta gcgcgcgttg atctcgccgg ccgcgtcacc ccgcttggcg gagcggaaga
     1141 actgcccgtc ggacgacgag gttgacccgt caccccatat ggctgcgatc ggcaaggcgt
     1201 ggtgcgcatt gatgatcctg gccagcgcct ccttgtaggt ttccgggcgg atataggcgt
     1261 cggcggtcca taacagttgg tcgcgggtga cgccctgact ggctgcagcc atgcgcgtca
     1321 ggcccaggtt ggaagcgtcg gccaggatgg ctgcgagcaa ggcgttctcg ttctcgcagc
     1381 actcgccagt acgcaggttg gtgaatgcgg cggtgaaacc cgtcgcgcga ctgacttcgt
     1441 gcatcacctc ggtaatgcgg gcgcgcggca acatcgcgtc gatccggtcg gccagcgcgt
     1501 cggcctccgg cggcgtggct gccttcactg gggagacatg caggcgcccg tcacgcaatt
     1561 cgaccccttc gagttcacct cgccgcagtt gccgggcgaa gcgcttcagc cgaccgtcga
     1621 gttcggcgcc acgtgctgcg agccattcat cggcagttgc gggcaagccc atgtcggccg
     1681 caattgcagg taccgccgat ggcggcagca gatagctgtc gaagcggcga taattggatg
     1741 atcgttcgac ccagacgtca ccggagcgca acctgtcgcg cagcgtggcg aacacggcgg
     1801 tttcgtagag ccgccggttt gtccgacctt cctcggaaac cagcagcttc cagtccttgc
     1861 ggaatggcat gggcgcgtcc gccggcacat cgcgcttgcc ggtgcggttg agatcttgca
     1921 gcagccgaag cgctgaaagg gtcgggtcat tgctccgggc tgccttgaat tccagcgcct
     1981 caatcagctc gggggcgaac ttgcggagtg tcagatagcg gtcagcagca cctcgcaagg
     2041 ggtcttcatc ggcgagattg gcgagcttgg tcacttcggc ctgaacccga agcagtttgg
     2101 cccagccaac ggcctcgtcg acaacggcga agccgtctcg tttgcttttc cgcgcctcat
     2161 cgagtgctgc gatcgtgtca tggaacaatc gcatgagacg gccgacatcc cttgtgctgg
     2221 ccacgtaacg tcgttccttg gccttccttg cccgagcaaa caggccgcca atcagcttat
     2281 ccgccatgtc aagcacagcg tcggtgatcc cggtctcgag atcgatgcag gtcgccacca
     2341 ggatcgctcg ccgccggtgt gcggcatagc gactgatctg gtgcgcatcc gatatgcgac
     2401 cttcccggac gagctgctga aagcgatctt catggacctg tgcggcagcg tcggcaggga
     2461 ggcctatgtc acggacagag cgcaaccggt cgagcaactc tcggatgttg tccgccttcg
     2521 gcgagaccgg cgtgttcctc agccacgcga gtggcgtcgc cccggtcgtc ggatcgacgg
     2581 tgaggagtcg atcgagttcg atgagttgtt cgctcgagac gcccgcaagc agggcgtccg
     2641 aggcgcgctt gcgggctcgg gcgcggccgg caatggcggc ccgctcgacc acggccggcg
     2701 caggaagaat gatcttctca gtgcggaggg cagagacaac gccggcggcg atcggctgac
     2761 ctcgatcggt gctccaagca gcctgcgccg cagcctcgat catgaagggc aaatcggctg
     2821 cgctcggtgg tcgcaatcca agcgttgcag cgaggatgcg cgcgtggtcg gtcatcgtct
     2881 gcggtcggcc ggcatactcg gcgaacacag cggcgcggat ctccagccgt gccgccagcc
//
LOCUS       DASWZT010000333         5914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_60683, whole genome shotgun sequence.
ACCESSION   DASWZT010000333 DASWZT010000000
VERSION     DASWZT010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60683"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2020
                     /gene="treZ"
                     /locus_tag="VGE92_09130"
     CDS             <1..2020
                     /gene="treZ"
                     /locus_tag="VGE92_09130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEY1314029.1"
                     /translation="VELVTPQRRVALHPADPQSTHAAGYWQAEVEASLLTDGYRYSLD
                     GAAPLPDPRSRWQPAGVHGSSHVVQLPPISAQFRAAPLSEAVIYELHIGTFTSAGTYA
                     AAQAKLPHLVDLGVTHVELMPLATFPGERGWGYDGVYLNAPFPAYGTPLELAAFVQAC
                     HARGIAVLLDVVYNHLGPDGNYLAQYAPYFTDRYKTGWGAAINYDGPHSDPVRRLVID
                     NALMWLRDYGFDGLRLDAVHAIFSCDAVHVLEELAAAVKQLGETLGRSFIVIAESDLN
                     DPRLVQPARRGGLGMDAHWADDFHHAVHRFFTGETLGYYQDFHGLADLATALRAGYVY
                     QGQYSPHRKRRHGRPPAGVAAQQLVVFAQNHDQIGNRAQGERLSMLLAAPQLKAIAAL
                     TLLSPFVPLLFQGEEWGAKTPFLYFTDHNAELGRLVAEGRSKEFSSFEWQGAVPNPQE
                     LATFTRSKLDWSELAQPAHADLYQWYRRLIELRRNHIARGKIAVSFDAKAGWLRFVRG
                     EICGVCNFSAAPLFARLPRGDWELVLRSDMHAAQPAERVPPHATLIYRRSDGAPKRQA
                     RGQRLAAPDDGRNADGAAIVGHIAKHDGICADHDIVADADRPKQLGPGPDIDVVADER
                     HAGAVGRPQADNDVVADTTVVAKSSIAADDDSAAMIDGQLPPNHRLAG"
     gene            complement(2374..2637)
                     /locus_tag="VGE92_09135"
     CDS             complement(2374..2637)
                     /locus_tag="VGE92_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314030.1"
                     /translation="MIEVNRVQTGVRIEESLLKVLKGLAEYSNMGLGDLLEGICLHAL
                     EGKTPFSRDTLQQIARLKQVYGLALSAADSHRLIEVRPKRRKT"
     gene            2655..3041
                     /locus_tag="VGE92_09140"
     CDS             2655..3041
                     /locus_tag="VGE92_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314031.1"
                     /translation="MTDEEFIAELEACILPESQFDHAAHVRAAYWYLGRYSFPDAAAR
                     MCSTLRNYTRAQGRQGRYHETLTIGFMAVILDHLRRRGDGGGWAGFRADNPELLRKDL
                     LLDYYPKAVLDSAAARERFILLPLIH"
     gene            3074..3775
                     /locus_tag="VGE92_09145"
     CDS             3074..3775
                     /locus_tag="VGE92_09145"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY1314032.1"
                     /translation="MSYIAERRGEEKERRRAEILNAAEALYAEKGWDSLTVDQVARSA
                     RLSRALVYVYFRDKEELLFAIGERAMSLLRDRFIAAGAGHELGLDQVTAIGQAYMAYA
                     NEFPHYFDFCSRFHAHSVAVDPGSHEGACQAAGDAAISAVIQAIETGKRDGSIRADVG
                     DSVLLATSLWAFTHGIIQLAMAKGDDLARFGITIPDFSTYAFGLLRTMTRSGTPAADP
                     SVSATGQSAASDQAR"
     gene            3798..5264
                     /locus_tag="VGE92_09150"
     CDS             3798..5264
                     /locus_tag="VGE92_09150"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY1314033.1"
                     /translation="MSSYSTGDVLRAFGMLLILAACPAPAIAAAGPALGSVEEAVSTL
                     VADALAANLELEGASAGVGERLAALDQARARYLPTLDFSARYSAADGGRTVDFPVGDL
                     LNPVYATLNQLTGSARFAPVANQEIEFQRTREQDTKLSLTQPLYDPRISAARAAAESQ
                     YESAAAGRAALAGRIERDMREAYYRWLQARAQIAIFAATLELATENHRVNDSLFRNGK
                     ITRDLVFRAEADQLEVEQSQLGARNSERLAQSYVNLLRNAPFDRALPETTVEDPDIDH
                     MRAGLAGRTAQPSLSLAPLQETALRRRAELKQLDAVAAAAAAGERLARAAFKPRLAFA
                     LDAGTQGDTYGIGSDDRYVLASLVLRFNLFSGGADQAGLAGARAVEREAHASRTAEEQ
                     TIRFEVQRALQDLELAEASLGTAAKRVEAAAGAFRIAARKRDLGQINQAEFIDSRRAL
                     TDAQLSLNVTRFSALSSLAELEYALGAGARDLPPESQP"
     gene            5291..>5914
                     /locus_tag="VGE92_09155"
     CDS             5291..>5914
                     /locus_tag="VGE92_09155"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEY1314034.1"
                     /translation="MAAIAASIHMQGCSRTTGAPLAAPMRTVRVTAVADAALDNTLRA
                     VGLLTPKDEARLSFKVGGLIESIQVEEGQSVKAGQVLAVLKPAEINAALEQARQAAAK
                     ARRDLDRGKALLADGVAAEEQVQDLTTAYQVASAAQGSAEFNAAHARIVATADGVVLR
                     KLAEANEIVQAGQTVVVLGGANRGWIVRVGLADRDVVRVRLGDAAQLE"
BASE COUNT         1043 a         1885 c         1932 g         1054 t
ORIGIN      
        1 ggtggaactc gtcacgccgc aacggcgggt cgcgctgcac ccggcggatc cgcaatccac
       61 gcacgccgcc ggctactggc aggccgaggt cgaggcatcg ctcctcacgg atggctaccg
      121 atattcgctc gatggcgccg cgccgctccc cgatccacgt tccagatggc agcccgcagg
      181 cgtgcacggc agctcgcatg tcgtgcaatt gccgccgatc agcgctcagt tccgcgccgc
      241 gccactctcc gaggcggtta tatacgagct gcacatcggc acgtttacca gcgcgggcac
      301 ctacgccgcg gcgcaggcca agttgccgca cctggtagat ctcggagtga cccacgtgga
      361 gctcatgccg ttggcaacct ttcccggcga gcgcgggtgg ggctacgacg gggtgtacct
      421 caatgcaccg tttccggcct acggcacgcc gctcgaactc gcagccttcg tgcaagcgtg
      481 tcatgcgcgc ggcatcgccg ttttgctgga cgtggtctac aaccatctcg gccccgacgg
      541 caattacctg gcgcagtacg ccccgtattt caccgatcgc tacaaaaccg ggtggggtgc
      601 cgcgatcaat tacgacggac cgcacagcga tccggtgcgc cgcctggtta tcgacaatgc
      661 gctgatgtgg ctgcgcgact atggcttcga cggcctgcgg ctcgatgcag ttcacgccat
      721 attcagttgc gacgccgtgc acgtgctcga ggaactggca gccgccgtga aacagctggg
      781 cgagacgctc ggccgcagct tcatcgtgat cgccgagagc gacttgaacg atccgcgctt
      841 ggtgcagccg gcgcgccgcg gcggcctcgg aatggacgca cactgggcgg atgactttca
      901 tcacgccgtg catcgctttt tcaccggcga gacgctcggt tactaccagg acttccacgg
      961 gttggcggat ttggcaacgg cgctgcgcgc cgggtacgtg tatcaggggc agtattcacc
     1021 gcaccgcaag cgccggcacg gccggccccc ggcgggtgtc gccgcgcagc agctggtcgt
     1081 gttcgcgcaa aatcacgatc agatcggcaa ccgcgcgcaa ggagagcggc tcagcatgtt
     1141 gctagccgcg ccgcagctga aggccatcgc ggcgctgacg ctgctctcgc ccttcgtgcc
     1201 gttgttgttt caaggggagg agtggggcgc caaaactccg tttctgtatt ttaccgatca
     1261 caatgcggag ctcggccgcc tggtcgccga ggggcgcagc aaggagttct caagcttcga
     1321 atggcaaggc gcagtgccga atccgcagga actcgcgaca ttcacgcgat ccaaactcga
     1381 ttggtcggaa ctcgcgcagc ccgcgcacgc agatctgtat cagtggtacc ggcggctcat
     1441 cgagttacgt cgcaatcaca ttgcgcgcgg caaaatcgcg gtgagcttcg atgccaaggc
     1501 gggatggctc agattcgttc gcggcgagat atgcggcgtc tgcaatttct cggcggcgcc
     1561 gctgttcgcg cggctgccgc gcggcgactg ggaattggtg ctgaggtccg acatgcacgc
     1621 ggcgcagcca gccgagcgcg tgccgcccca tgcgacgctc atctaccgcc gcagcgacgg
     1681 ggcgcccaag cgccaagcgc gcggccagcg gctcgcggct cctgacgatg gccggaacgc
     1741 cgacggcgct gcaattgtcg ggcacatcgc aaagcacgac ggcatttgcg ccgatcacga
     1801 cattgtcgcc gatgcggatc ggcccaagca gcttggcccc ggccccgata tcgacgttgt
     1861 tgccgatgag cggcacgccg gggcggtcgg gcgacctcaa gccgacaacg acgtcgttgc
     1921 ggatacgaca gtggtcgcca aatcgagcat agccgctgac gatgattccg ccgcaatgat
     1981 cgatggtcaa ctgccgccca atcatcgcct cgcagggtaa ttcgatttcg gtcaggatct
     2041 ggatcagctt gaacaagatc ttgtagaaca gcgagaagac tttgcgcagc caggaggggc
     2101 gcacgcggca acgccagcgg ccgaaccggc agatcaccag cgcccaaaat ccttgagcgc
     2161 cgatgcgccc gccattggcg cgaaaatcct gccggatgtt ctcaaacata ccccggcatc
     2221 gacctccgcg agtgactcag cgtcatgcca tcggtcggat cgcagcgtgc gccgtcgggt
     2281 tgcgggcttt agagatgaaa tttatttgtc ggactcgcct ccccgctcat gcgttggagt
     2341 tgcccgggac ccggtatatt ggtaccatgg tacctaagtc ttgcgccgtt tgggccggac
     2401 ctcgatcagg cggtggctgt cggcggcgct caatgcgaga ccataaacct gtttcaggcg
     2461 tgcaatttgt tgcagagtgt ctcgtgagaa cggcgtcttg ccttcgagcg catgcaggca
     2521 gatgccttcc aagagatccc cgagtcccat gttcgagtac tcggcgagtc ctttcaggac
     2581 tttgagcaag gattcctcga tacgaacacc ggtttgcact cgattgactt cgatcataag
     2641 tagggcctgc gctgatgacg gacgaagaat tcatcgccga actcgaagcc tgcattctgc
     2701 cggaaagtca gtttgatcac gccgcgcacg tgcgcgcggc gtattggtac ctgggacgct
     2761 actcatttcc cgacgcggcg gcgcgcatgt gcagcacctt gcgcaattac acgcgagctc
     2821 agggtcggca gggccgctac cacgagaccc tgacgatcgg cttcatggca gtcatcctgg
     2881 atcatctgcg gcgtcgcggc gatggcggtg ggtgggccgg cttcagagcc gacaatcccg
     2941 agctgctgcg caaagatcta ctgctcgact actacccgaa agccgtactg gactcggcgg
     3001 cggcccgcga aagattcatc ctgctgccac tcattcattg acactgtgtc aattattgcc
     3061 taccgttcag cgcatgtctt atattgcaga acgtcggggg gaagagaaag agcgccgtcg
     3121 cgcggagatc ttgaatgccg ccgaagcgct gtatgccgag aaaggctggg actcgctgac
     3181 ggtggatcag gtagcgcgca gcgcgaggct ttcgcgggcg ctggtctacg tctactttcg
     3241 cgacaaggaa gagctcttgt ttgccatcgg cgagcgcgcc atgagcctgt tgcgcgatcg
     3301 cttcatcgcg gccggcgccg ggcatgaact gggccttgac caagtcacgg caatcggtca
     3361 ggcgtatatg gcatatgcca atgaatttcc acattatttt gacttctgtt cgcgcttcca
     3421 cgcgcactcg gtcgcggtcg atccgggctc gcacgagggt gcctgccagg ccgccggcga
     3481 tgcggccatc agcgcggtga tccaagcaat cgaaaccggc aagcgcgacg gcagcattcg
     3541 cgccgacgtt ggcgactccg tgttgttggc gacctcgctg tgggctttca cgcacggcat
     3601 catccaattg gccatggcca aaggcgacga tctggcccga tttggaatta cgattcccga
     3661 tttcagtacc tacgcattcg ggttgctgcg gaccatgacc cgcagcggca cgccggcggc
     3721 tgacccctcg gtgtcggcca cagggcagtc ggccgcgtcg gaccaggcgc ggtaaatttt
     3781 tgctccggtg ttgacacatg tctagttact ctacaggtga cgtactgcgc gcgttcggca
     3841 tgttgctgat tttggcagcc tgccccgccc cggccatcgc ggcggcgggc cccgcacttg
     3901 gctcggtgga ggaggccgtt tcgacgctgg tggccgacgc gcttgcggcc aatctcgagc
     3961 tggaaggggc gagcgccggg gtcggcgagc gcttggcagc cctcgatcag gcccgcgcac
     4021 gatacctacc cacactcgat ttctcggcgc gctactcggc cgccgatggc ggccggaccg
     4081 tggactttcc ggtcggcgac ttgctcaatc ccgtgtacgc gaccttgaac caactgacgg
     4141 gatcggcgcg ctttgcaccc gtcgccaacc aagagatcga atttcaacgc acgcgtgaac
     4201 aggataccaa gctgtcgctg acgcagccgc tgtacgaccc gcgaatcagc gccgcgcgcg
     4261 ctgccgccga gtcgcagtac gaatccgccg cggccggccg tgcggcgctc gccgggcgga
     4321 tcgagcgcga catgcgcgag gcctattacc gatggctgca ggcgcgcgcg cagatcgcga
     4381 tcttcgctgc gaccttggaa cttgcgaccg agaatcatcg cgtcaacgac agtttatttc
     4441 gcaacggcaa gataacccgc gacctggtat ttcgcgccga agccgatcag ctcgaagtgg
     4501 agcagtcgca actcggggcg cgcaacagcg agcgcctggc acaaagctat gtgaatctgc
     4561 tgcgcaacgc cccgttcgat cgcgcgctgc ccgagaccac ggtcgaggat cccgatatcg
     4621 accacatgcg cgcgggactc gccggccgga ccgcacagcc ttccttgagt ctcgcgccgc
     4681 tgcaggaaac ggcgctgcgg cggcgggcgg aactcaagca gttggacgcg gtggcggccg
     4741 ccgccgccgc cggtgaacgc ttggcgcgcg ccgccttcaa accgcggttg gcgtttgcgt
     4801 tggacgccgg cacgcagggc gacacgtacg ggatcggcag cgatgatcgc tacgtgctgg
     4861 cctcgctggt gctgcgtttc aatttgtttt ccggtggtgc cgatcaagca ggcctcgccg
     4921 gcgcgcgcgc cgtggagcgc gaggcccatg ccagccgcac ggccgaagag cagacgattc
     4981 gattcgaagt gcagcgcgcg ctccaggacc tcgagctcgc cgaggcatcg ctcggcaccg
     5041 ccgccaagcg ggtcgaggcg gctgcgggcg cctttcgcat tgcagcgcgc aagcgcgatc
     5101 tcggtcagat caatcaggcc gagttcatcg actcgcgccg cgcgctcacc gacgcgcagc
     5161 taagtctcaa cgtcacgcgt ttctccgcct tgagcagtct cgccgaactc gagtacgccc
     5221 ttggcgcggg tgcgcgcgat ttaccgccgg aatcgcagcc atgaaacaac atagatcaat
     5281 ggtactgggc atggccgcga ttgcggcgag cattcacatg caaggctgct cgcgtacgac
     5341 aggcgcaccg ctcgcagccc cgatgcgcac ggtgcgggtg accgcggtcg cggacgcggc
     5401 gctcgacaat accttgcgcg ccgtgggctt gctcacgccg aaggatgagg cgcgcttgtc
     5461 gttcaaggtt ggcggcctga tcgagtcgat tcaagtggag gagggccaga gcgtcaaggc
     5521 ggggcaggtg ctggcggtgc tgaagccggc ggaaatcaac gccgcgctcg agcaggctcg
     5581 gcaggccgcg gccaaggcgc gccgcgatct cgaccgcggc aaggcgttgc tcgccgacgg
     5641 cgtggcagcc gaggagcagg tgcaggatct gacgaccgcg tatcaggtgg cgagcgccgc
     5701 gcagggcagc gctgaattca acgccgccca cgcgcgcatc gtcgccacgg ccgatggcgt
     5761 ggtgttgcgc aagcttgccg aagcgaatga gatcgtgcag gcgggtcaga ccgtggtggt
     5821 attgggcggc gccaatcgcg gctggatcgt gcgggtgggg ttggccgatc gcgatgtggt
     5881 gcgagttcgg ctcggcgacg cggcgcaact cgag
//
LOCUS       DASWZT010000334         2852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_2662, whole genome shotgun sequence.
ACCESSION   DASWZT010000334 DASWZT010000000
VERSION     DASWZT010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2662"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1381)
                     /locus_tag="VGE92_09160"
     CDS             complement(<1..1381)
                     /locus_tag="VGE92_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314035.1"
                     /translation="MNAPYVFLAARRVPGVAWLICAALALAGCNGTAVVTMTSTPSSD
                     NFLAYRVALVAVQLQTSGGKSALAVLPESTVVDFASLTSLSEVLGAASISKGTYTSAL
                     ITLDYSSAQIVYDDGSANGVALTPVGAKGQALGQVQLTVSLDPSDSFSVSSKGASQLA
                     LDFNLAASNIVNTSAKTVTVTPLIAASAMPIDSKLVRVRGQLESVAVDSSGATNGAFT
                     MSVMPFNSISNGTGRLSIVPASVTAYEISGSGSTGSTGLGQLAALDTGTLVAAYGSLV
                     ETTDATTTTTTTDGVTTTTTDGDVTTTTSAPTVSFAATQVLAAGSVQGAGLDRVSGIV
                     AARSGNTVNIEDATLIGADGSNTFLGGTTSVIMGSNTLVTEFGQGGAAIDTIAQVSVG
                     SAIDAFGVLTGQASDNAILDASAGRVRLDVSGASGLVTAQGSGTLNINLAYLGGRSVA
                     ALDFVGSGAA"
     gene            complement(1548..1703)
                     /locus_tag="VGE92_09165"
     CDS             complement(1548..1703)
                     /locus_tag="VGE92_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314036.1"
                     /translation="MAKPNYRQAKKQKEAARKARQDAKQQRRSTGPEAADASVAPLQP
                     EPAKPAE"
     gene            complement(1738..2634)
                     /locus_tag="VGE92_09170"
     CDS             complement(1738..2634)
                     /locus_tag="VGE92_09170"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY1314037.1"
                     /translation="MSGSMRYRVRHETVYHYGGNVAHSHQLLHLSPRDSAHQVCRSRS
                     IALDPAPSTRREDLDAFGNLVTRLEYDLPHDRLAVLAEVGVEVSVPRHAAQTESTAWE
                     EVRDALIYSGHAMSAQYLDAGRFRMESSYVRVKQSFSEYADDCFSPQQPLLQGAMALM
                     HKIHRDFEYAPGSTNIRTSVIEAFAARRGVCQDFAHIMLACLRSRGLAARYVSGYLRT
                     FPPAGADPVLLGADASHAWVSVFCPPFGWVDLDPTNDVRVATDHIVIAFGRDFGDVSP
                     LRGVIVGGGKHRLSVRVSVQPE"
     gene            complement(2631..>2852)
                     /locus_tag="VGE92_09175"
     CDS             complement(2631..>2852)
                     /locus_tag="VGE92_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314038.1"
                     /translation="LGGSPDDTLDEAVALVEEMQLAAIEGDSSRAARARQALAEQLNG
                     LSVAAGMLSDRLSLKHFSLIDTETRTVAA"
BASE COUNT          485 a          918 c          952 g          497 t
ORIGIN      
        1 cggccgcacc gctgccgaca aaatcgagcg ccgccacgct gcggccgccg agataggcaa
       61 ggttgatatt cagcgttccg gagccctgcg cggttaccaa gccggaagcg ccgctgacgt
      121 cgaggcgcac ccgtccggcg cttgcatcca aaatggcatt atccgatgcc tggccggtga
      181 gtacgccgaa ggcatcgatc gcggaaccca cggagacctg cgcgatggta tcgatcgcgg
      241 caccgccctg tccaaactcg gtcacgagcg tgttcgaacc catgatcacc gaggtcgtgc
      301 cgccgaggaa agtattgctg ccgtctgcgc caatcagcgt cgcatcctcg atgttgaccg
      361 tgttgccgct gcgtgcggcc acgatccccg acacgcggtc caaacccgcg ccctgcacgc
      421 tgccggccgc cagcacttgc gtggcagcaa aggagaccgt cggcgcactg gtcgtcgtcg
      481 tgacgtcgcc gtcggtcgtg gtggtggtga cgccgtcggt cgtcgtcgtg gtggtggtcg
      541 cgtcggttgt ctcgacgagg ctgccgtagg ccgccaccag cgtcccggtg tcgagtgccg
      601 cgagctgccc gaggccggtg ctcccggtcg agccggaacc gcttatttcg taggcggtga
      661 cgctggccgg cacgatcgac aggcgtcccg tgccgttgct gatgctgttg aacggcatga
      721 cgctcatggt gaacgcaccg ttggtcgcac cgctgctgtc cacggcgaca ctctcgagct
      781 ggccgcgcac gcgcacgagc ttcgagtcga tcggcatggc gctcgcggcg atcagcggtg
      841 tgaccgtcac cgtcttggcg ctggtgttga cgatattgct ggcggccagg ttgaagtcga
      901 gtgccagttg cgaggcgcct ttcgaggaga cgctgaatga gtcgcttgga tcgaggctca
      961 cggtgagctg gacttgtccc aaggcttggc cctttgcgcc gaccggcgtc aacgccacgc
     1021 cgttggcgct gccatcgtcg tacacaatct gcgccgagct gtagtccaac gtgatcagcg
     1081 cgctggtata agtgcccttg ctgatcgagg ccgcgcccaa cacctcgctc agactggtca
     1141 ggctcgcaaa gtcgacgacg gtgctctcgg gcagcaccgc gagtgcggac ttgccgcccg
     1201 atgtttgcag ctgcaccgcc accagtgcga cgcggtacgc gaggaaatta tccgagctcg
     1261 gcgtgctcgt catcgtcacg accgcagtgc cgttgcagcc ggcgagcgcc agcgccgcgc
     1321 aaatgagcca cgcgacgccc ggcacgcggc gcgcggcgag aaaaacgtac ggcgcgttca
     1381 tggttggatt gaccctccgg gcaggccaat tgttcagcga tttgcgccgg ttttgaagcg
     1441 cctgcagggc aaagatctgt gaaggcgcgc aaaacaaaac gcagcggcgt cgccctgcgg
     1501 cgacagcgcc gcgggagcgt gcgcaaagcg gcaggttacg ggttatgtca ctcggcgggt
     1561 ttggcaggct cgggctgcaa tggcgcgacc gacgcgtcgg ccgcctccgg accggtcgag
     1621 cggcgctgct gcttcgcatc ctgacgagct ttcctggcgg cctctttctg tttctttgcc
     1681 tgacgatagt ttggcttggc caagggggtc tcctgaggat gaattgtgtt ttcgatctta
     1741 ctccggctgg accgaaacgc gaaccgataa acgatgcttg ccgccgccga cgatgacgcc
     1801 gcgcagcggc gacacatcgc cgaagtcgcg cccgaaggca atgacgatgt gatcggtcgc
     1861 tacccgcaca tcgttggtcg gatcgaggtc gacccagccg aacggcggac aaaacaccga
     1921 aacccacgcg tgcgacgcgt ccgctccgag caacacggga tcggctcccg ccggcggaaa
     1981 ggttctgagg tagccgctga cgtagcgggc cgcgaggccg cgcgagcgca ggcaggcgag
     2041 catgatatgc gcgaagtcct ggcagacgcc gcggcgcgcc gcgaaagcct cgatcaccga
     2101 ggtacggatg ttggtcgatc ccggcgcata ctcgaagtcg cggtgaatct tgtgcatcag
     2161 tgccatggct ccctgcaaca gcggctgctg cggcgagaag cagtcatcgg catactcgct
     2221 gaaggattgc ttgacccgga cgtagctcga ttccatgcgg aatctgccgg catcgagata
     2281 ctgcgcgctc atggcatgac ccgaatagat gagcgcatcg cggacctcct cccaagcggt
     2341 ggattcggtt tgcgccgcgt gccgcggcac actcacctcg acgcccacct ctgccaatac
     2401 ggccaggcgg tcatgcggca gatcgtactc gagacgcgtg accagattgc cgaacgcatc
     2461 gaggtcttcg cggcgcgtcg agggcgcggg gtcgagcgcg atcgatcgcg agcggcatac
     2521 ctgatgcgcc gagtcgcgcg ggctcaaatg caacagctga tgcgagtgcg cgacgttgcc
     2581 cccgtagtgg tacacggttt cgtgccgcac gcggtaacgc atgctgccgc tcacgcggcc
     2641 accgttctgg tctcggtgtc gatcagcgag aagtgtttca acgacaggcg gtcggacagc
     2701 atgccggcgg ccacgctcaa gccattgagc tgctcggcaa gcgcctgtcg cgcacgcgcg
     2761 gcgcggctgc tgtcgccctc gatggccgcg agttgcatct cctcgaccag agcgacggcc
     2821 tcatcgaggg tgtcgtccgg cgagccgccg ag
//
LOCUS       DASWZT010000335        13139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_55898, whole genome shotgun sequence.
ACCESSION   DASWZT010000335 DASWZT010000000
VERSION     DASWZT010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13139
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55898"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VGE92_09180"
     CDS             <1..922
                     /locus_tag="VGE92_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY1314039.1"
                     /translation="VAPVIMAFGSVAQQQRFLPKISAGAEWWCQGYSEPGAGSDLASL
                     KTRALLEGDHYVVNGQKTWNTLGQFADWIFCLVRTDSHAKQQSGITFLLIDMKTPGIT
                     VRPIMLLDGGHEVNEIWFENVRVPVENRIGEENRGWTYAKFLLGHERTNIAGIGIAKR
                     ELARLKRIAANERQHGRPLLEDPLFAAQIAQIEIDLWALEITNLRVLSAERKSPAAGP
                     EASILKIRGTEIHQAISELLMRAVGPYALPFRREAMEAGWQFDPTRESPGPRYASTLA
                     ASYFNQRKLSIFGGTNEIQKNIIAKMIVGL"
     gene            932..2071
                     /locus_tag="VGE92_09185"
     CDS             932..2071
                     /locus_tag="VGE92_09185"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY1314040.1"
                     /translation="MNFEFSDEQRQLHDVVERYLNEQYGFERYQSIKNSAAGWDSSVW
                     RGLAELGVLAITVPVEQGGLGFGPLETLAMMGDCGRSLLLEPVLSSAVIGSAVLRAFA
                     DSAPAAQLLTAIGSGEKIAVLAHFESGARYESQWVSTSARRNGDNYQLAGHKAVAMHA
                     GAADTLLISARTSGETGDANGVSLFLVPRTAPGVVLDEYPIVDGERAADVYLKGVDVA
                     VAQRLGPEGGALTAIEAALDIGVAALCADAVGVMQAMVDATVEYVRQRQQFGQPIGRF
                     QVLQHRIADMLIHLEQARSMSYLAALQCTDENIRERRRALSAAKVVIGQAGRFIGQQA
                     VQLHGGMGMTDELKISHWFKRLTAAQLMFGDSDTHLQRFASLNQA"
     gene            2068..3174
                     /locus_tag="VGE92_09190"
     CDS             2068..3174
                     /locus_tag="VGE92_09190"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEY1314041.1"
                     /translation="MSQRVRAVVAREVGKPPTVEEIEVEAPRYGEVTLKLAACGVCHS
                     DLSATNGTIAYPLPLVLGHEGAGVVTAVGDGVSRYAVGDHVVSSFVSMCGRCHYCQTG
                     RPQLCVQSLKALYTLPDGTVRTRDGAGYALNVFCGCGVMAEFATLHADNLVKIDAEMP
                     LDRAALIGCGVMTGVGAVVNTARVEAGSVTVVFGCGGVGLNAIQGCAIAGAGMIVAAD
                     TSEAKLELAKKFGATHTVNVVGQDNVGKVLYKLTGGGADYAFDCVGLGKISEQAWGVL
                     RRGGTAVIVGIAAPKDQITLNAQQVALSEKTLTGSYYGSARPAQDFPRLIGLYRSGRL
                     KLDELITRTYTIDEAPQAFADLVAGREGRGVIVF"
     gene            3463..4356
                     /locus_tag="VGE92_09195"
     CDS             3463..4356
                     /locus_tag="VGE92_09195"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY1314042.1"
                     /translation="MPEAGLVIETRGENLLAVRFTAMASPCEVLLPAMSKQGALAIGA
                     LAAQEAWRVEKKYSRYLEQSVTAWIHANRGTSIEVDEETASLIEFARQCFDLSEGLFD
                     ITSGVLRGAWKFDGSDRIPEVAAVERLLPLVGFEKLQWRSPRLLLPPGMELDFGGIGK
                     EYAVDRACELLAARDAIPFLVNFGGDLRANRPPSHGPWQVGIEAPDSDREASMLLDLE
                     SGALATSGDSRRYLLKHGIRYGHILNPLTGWPDPESPRSITVAASSCTEAGLLSTVAM
                     LHGAGARAYLEEQGARYWLVE"
     gene            4470..5312
                     /locus_tag="VGE92_09200"
     CDS             4470..5312
                     /locus_tag="VGE92_09200"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY1314043.1"
                     /translation="MFSAVARLATRGVRYDKTRNATRMKWLMFGWAVVGIFPVVGRTA
                     DSLLDSTLEDTKLYFTSPLRWDEQDWLYFGGALAAIGAAHSFDERVRDHFATGSRAIL
                     NGGADKNSLRDAAPTVAIIAGTGLYAAFIDDHDGYREAWSLIEAGAFSGATAEVLGYA
                     TGRERPDATTSPNEWGKGSDSFPSLHTTVAFAVGTVFAESGNDEYRWIRRIIGYGVAG
                     ATGYIRVNENVHWLSDSVAGAALGIATARFVLNRQGAQDNRAALQFQPIKNGWMISYS
                     MRTH"
     gene            complement(5329..6513)
                     /gene="chrA"
                     /locus_tag="VGE92_09205"
     CDS             complement(5329..6513)
                     /gene="chrA"
                     /locus_tag="VGE92_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019396533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEY1314044.1"
                     /translation="MHEGSALEVFLSFLKLGLTSFGGPVAHIGYFREEIVVRRRWVDD
                     ATYTDLVALCQFLPGPASSQVGFSLGLIRAGYWGALAAWAGFTLPSAVLMTAFAYGAN
                     ALGSATGSGILHGLKLVAVAIVAQAVWGMAATLCPDRQRASIACVAAVIVLSGNSSTA
                     QIGVILMGGIAGFWLCRASAPTVSRPLGIPVSRRVGIVALAAFFVLLAVLSVLGMRYR
                     GFELFDAFYRSGALVFGGGHVVLPLLSKAFVTPGWVSQDSFLAGYGAAQAMPGPLFTF
                     AAYLGAVGNHSPRGIAGAALGIAGIFLPGFLILLGALPFWDTLRQRIGARAIMRGINA
                     AVVGLLGAALYGLLWVNAVGSSGDFALALTGFVLLTAWRAPPVLVVILGALGGIALTQ
                     YV"
     gene            6867..7256
                     /locus_tag="VGE92_09210"
     CDS             6867..7256
                     /locus_tag="VGE92_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1314045.1"
                     /translation="MTVHAIKVLLVEDSSVLAERLAETITEFPEIDLINSVDSESSAL
                     AEIRKRRIDVVVLDLQLKEGSGFGILRAVAGMQQKPCVVVLTNHDSSAYEREAVSLGA
                     RYFLDKARDFHRLPDILREIVLCGVTT"
     gene            7262..8566
                     /locus_tag="VGE92_09215"
     CDS             7262..8566
                     /locus_tag="VGE92_09215"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEY1314046.1"
                     /translation="MGLSLTIIANISDAVLIDRLQRAAYDYFGPNSNPDNGLVADTSR
                     ATSPSSIAVVGFGLSCYPVAVERGWIGRDSAAARVLATLRFFWDSEQSEQRNATGYRG
                     FYYHFLDMQSGRRAWECELSLIDTALLLAGVLTAALYFDGEDSEAQIRELARALYERV
                     DWTWALNGGETLAQGWRPETGFLHYGWEGYNEATLLYLLGLASPTHPLPAASFASWTF
                     TYQWENLLGIDTLYSGPLFTHLFSQAWVDFRGIRDPFMRQAGSDYFANTQSAIAIQRE
                     YAARNPRGFVGYSRNLWGITAGDGPANADNVRRSRDRRYFGYMARGVPFGPDDGTLAP
                     WAMLASLPFNPDAALEGTRHVLQRHPEVCPQDRFVSGFNPSRITKKKPWLSQGWYGLD
                     QGLLVMMLENHRSGLIWNLTRASPVFRSGLQRAGFKGGWLDS"
     gene            8563..10023
                     /locus_tag="VGE92_09220"
     CDS             8563..10023
                     /locus_tag="VGE92_09220"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1314047.1"
                     /translation="MTQRVDVAYLERVRPPVWHNPAPSGHYDLVILGGGPAGLTAARV
                     ARSLGLSVALVERARLGGNSLNAGSIPSKALVRSGRAFAAMLNGRRFGAPDAGEPMAD
                     FSAVMRRMHAVRSRIAESCSALRLTAMGVDVFFGHAEFAQDRCMQVGAAALHFRKALI
                     AAGARPRAAAIPNLEAVGYLTSNSFFEIERLPRRLAVIGGGPLGCEMAQAFAHMGSRV
                     TILQDEPKFLPREERDAAEILSLSLARSGVETHLNTTIVGARAANGEKLIDFENTGVS
                     RTLAVDEILVSIGRVANSDELRLAAAGIATAADGRIQIDDFLTTSNPDVYAAGDVCMA
                     HKNTNVAQASAEMAVRNAFAMAEQRHSSIVAPRCTYCDPEIAHIGMHVWDAARQGIAV
                     KTYTIMMQDVERAIIDGRVDGFVKIHLRAGSDEIIGASIVASRASEMINEVAVIMHAK
                     LGMRALAGILHAYPAQSDAIRLAACAYMNSLNHAVR"
     gene            10010..>13139
                     /locus_tag="VGE92_09225"
     CDS             10010..>13139
                     /locus_tag="VGE92_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ndvB"
                     /protein_id="HEY1314048.1"
                     /translation="MQFASALRNAGAPGRGGASGPGEILSVERLEVYAENLARTQEVF
                     PTRKAGISFSGRLKSNAIALNGAFQALVVGIRKGETVTPAAAWLVDNFYIIDEHLKAI
                     RRDLPPGYYKQLPKLANGTLRGYPRVYGIAAGLVDHTDNRFELDTLYRYVQAYQRIQP
                     LTIGELWAIAITLRVILIDNLRDLAVGIVSRLSEREQADALADQWLADDSTAAQSRFL
                     PATRLTPPFAAQLFRRLRDHDPSTTPALAWLHQTLAAQNWSADEIVHSEHQRQGAANV
                     SVRNIITSLRLISSVDWAHFFESVSPVDELLRTQSAYGSFDFLTRDLYRHAIEELARR
                     CNHTELEVARRAIQAAERAAGGAATQGGAGSAPREPGYYLVCAGRPQFERELGYKRAF
                     RERVARFTRRAGVLGYVGTIFLLSSAFSLALVWEVGLSGRDIGVSVALTGLALLAATD
                     IGIAIVNLWVAAFCPARALPALELEDGVPSELRTLLVIPILLTTLAELEESIRNLEVH
                     YLATHEGDIRFALLSDWCDSPEKIAPADEELLAAAEAGIARLNRTHGTPEDGARFLLL
                     HRRRMWNASEQRWMGWERKRGKLHELNLLLRGSQDTSFLGSAARPLAVPAGVRYVISL
                     DADTRLPRGAARALIGKMAHPLNQPVIDPVRRRVTSGYGILQPRVTPSLPAGSDASFF
                     QRVSSGPSGMDPYAFAVSDVYQDMFGEGSYSGKGIYDVTAFEAALENRVPENRLLSHD
                     LFEGSYARCGLASDVEVIEEYPSRYDVASARQHRWTRGDWQLLPWVLHDGSLTALARW
                     KMIDNLRRSLIAPAVFLGLALSWAAAAAAAWTAFFLATLSLPVLLPLVVGWLARVEIG
                     RSVFRPARKEAIQALSLIALRLIFLADQACSAGDAIVRTLYRLAISHRHLLEWTTSAQ
                     AKRRGFTATSTLFGAMAAGWLLSAATALVLIAARGRVPWESVPWLAAWFAAPLIARRI
                     SVRAAKPIAEALTHAETRELRDMAKQTWGYFDTFVSAQQNHLPPDNFQEIPSAVIAHR
                     TSPTNVGLYLLSV"
BASE COUNT         2474 a         3944 c         4260 g         2461 t
ORIGIN      
        1 ggtcgcgccc gtgatcatgg cgttcggcag cgtcgcccag caacagcgct tcctgccgaa
       61 aataagtgcc ggcgccgaat ggtggtgcca gggatattcg gagccgggag ccggctccga
      121 tttggcgtcc ttgaagacac gcgcgctgct cgagggcgat cactatgtgg tcaacggcca
      181 aaaaacctgg aatacgctcg gtcagtttgc cgattggatc ttttgtttgg tgcgcaccga
      241 cagccacgcc aagcagcaat cgggcattac gttcttgctg atcgacatga agacccccgg
      301 catcacggtg cgtccgatca tgctcttgga cggtggccat gaggtcaacg aaatttggtt
      361 cgagaacgtg cgcgtgccgg tcgaaaaccg catcggcgag gaaaaccgcg gctggaccta
      421 tgccaagttc ctgctcggtc acgagcgcac caatattgcc ggtatcggca tcgccaagcg
      481 cgaattggcg cgcctcaagc gcatcgcggc gaacgaacgc caacatggac ggccgttgct
      541 cgaggatcct ttgttcgccg cgcagatcgc acagatcgag atcgatctgt gggcgctcga
      601 gatcaccaat cttcgggtgt tgtcggcgga gcgaaagtcc ccggcggcgg gtccggaggc
      661 ctcgatactg aaaatccgcg gcaccgagat ccaccaagcg atttccgaac ttctgatgcg
      721 ggcggtcggg ccttatgcgc tgccgtttcg ccgcgaagcg atggaagcgg gttggcagtt
      781 cgatccaacg cgcgaatcgc cgggtccgcg ctatgcgtcg actctggcgg cgtcgtattt
      841 caatcagcgc aagctgtcga tattcggcgg caccaacgaa attcagaaaa acatcatcgc
      901 gaagatgatc gtcggtctgt aggagaattc cttgaacttc gaattcagcg acgaacagcg
      961 gcagctgcac gacgtcgtcg agcgctactt gaacgagcag tacggcttcg aacgctatca
     1021 gtcgatcaag aattccgccg cgggctggga ttcatcggtc tggcgcggcc tcgccgagtt
     1081 gggcgtgctt gccatcacgg tgccggtgga gcaaggcggt ctcggatttg ggccgttgga
     1141 aacgctcgcc atgatgggcg actgcgggcg gagtctgctg ctcgaaccgg tgctcagcag
     1201 cgcggtgatc ggcagcgccg tattgcgtgc atttgccgat agtgcgccgg cagcgcaact
     1261 cctcaccgcc atcggctccg gcgagaagat cgcggtgttg gcacatttcg aaagcggcgc
     1321 gcgctacgag agccaatggg tgagcacgag cgcgcgtcgc aacggcgaca actaccaact
     1381 cgcgggacac aaggccgtgg cgatgcatgc cggcgcggcc gatacgctgc tcatctcggc
     1441 gcgcacttcc ggcgagacgg gagatgcgaa cggagtgtcg ttgtttttgg tgccgcgcac
     1501 ggcgcccggc gtggtgctcg atgagtatcc gatcgtcgac ggtgagcgcg ccgcggatgt
     1561 gtacttgaag ggcgtggacg ttgctgtggc acagcgtctg ggtcccgagg ggggcgcatt
     1621 gacggcgatt gaagcggctc tcgacatcgg cgtcgcggcc ttgtgcgccg atgcggtggg
     1681 cgtcatgcag gcgatggtgg atgccaccgt cgaatacgtg cgccagcgcc agcaattcgg
     1741 tcaacccatc ggccgctttc aagtgttgca gcatcgcatt gccgacatgc tgatccatct
     1801 cgaacaggcg cgctccatga gctatcttgc cgcgctgcag tgtactgatg agaatatccg
     1861 cgagcggcgc cgcgcgctgt cggcggccaa ggtcgtgatc ggccaggccg gccgattcat
     1921 cgggcaacag gcggtgcagt tgcacggcgg catgggcatg accgatgagc tcaagatcag
     1981 ccactggttc aagcggctca ccgccgctca attgatgttt ggcgacagcg acacacattt
     2041 acaacgcttc gcgtccctca accaggcatg agtcagcgag tgcgggcggt ggtggcgcgc
     2101 gaagtcggca agccaccgac ggtcgaggaa atcgaggtcg aggcgccgcg ttacggcgaa
     2161 gtcacgctca agctcgcggc atgcggtgtg tgccacagcg acttatcggc caccaacggc
     2221 acgattgcat atccgctgcc gttggtgctc ggccatgaag gcgcgggtgt ggtgacggcg
     2281 gtcggcgatg gcgtcagccg ctatgcggtc ggcgatcatg tggtcagctc cttcgtcagc
     2341 atgtgcggcc gctgtcacta ttgccagacc ggccgtccgc aattgtgcgt gcagtcgctc
     2401 aaggcgctgt acacgctgcc ggacggcacg gtgcgaacgc gcgacggcgc cggatacgcg
     2461 ctcaatgtct tttgcggctg cggcgtgatg gccgagttcg cgacgctgca tgccgataac
     2521 ttggtgaaga tcgatgccga gatgccgctc gatcgcgccg cgctcatcgg ctgcggggtg
     2581 atgacgggtg tcggtgcggt ggtcaatacg gcgcgggtcg aagcgggttc ggtgacggtg
     2641 gttttcggtt gcggcggagt cggcctgaac gcgattcagg gatgcgccat cgcgggagcc
     2701 gggatgatcg tcgccgccga taccagcgag gcgaaactcg aattggcgaa aaagttcggc
     2761 gcgacgcaca ccgtcaacgt cgtcggtcag gacaacgtcg gcaaggtgct gtacaaactc
     2821 acgggcggcg gtgcggatta cgccttcgat tgcgtcggct tgggcaagat atccgagcag
     2881 gcgtgggggg tgctgcgccg cggcggtacc gccgtgatcg tcggaatcgc ggccccaaaa
     2941 gatcagatca ctttgaatgc acaacaagtg gcgctcagcg aaaaaacctt gaccggcagc
     3001 tattacgggt cggcacgacc cgcccaggat tttccgcgct tgataggttt gtatcgcagc
     3061 ggccgcctga agctcgacga attgatcacg cgcacctata cgatcgatga ggcgccgcag
     3121 gctttcgccg atctcgtcgc ggggcgcgaa ggtcgcgggg tgatcgtttt ctaggacggc
     3181 gttgcccgcg tggcaatcaa tcccgcttga cacccatata ctttgcaata caaagtatat
     3241 gcatctgcgg gtgtacctcc ctgggtgtca agcccgctcg acgcggccat cgatgaccat
     3301 gtgtacttca gcaaagaggc ctcctcgggc ggccgttcgt tggcgggagg cggctgcgga
     3361 tgcaattgaa gccaaaatcg ccgccgctca aggcggggac atggcggttc taacgtattg
     3421 accgacggct gcgcgaggct cgcgcggcgg aggcagcttg aggtgcccga agcagggtta
     3481 gtcatcgaga cccggggcga gaatctcctg gcggttcgat ttaccgcgat ggcgagcccc
     3541 tgcgaggttc tgctgcctgc aatgagcaag cagggagcct tggcgatcgg cgcgctggcg
     3601 gcacaggaag catggcgtgt cgagaagaag tacagtcgct acctcgagca gagcgtcacc
     3661 gcctggatcc acgcaaatcg gggcacgagc atcgaagtag atgaggaaac agcctcgctg
     3721 atcgagtttg caagacagtg cttcgatctc agcgagggtc ttttcgatat cacctccggc
     3781 gtgctgcgcg gcgcctggaa attcgatggg tcggaccgca ttcccgaggt tgctgcggtc
     3841 gagcgtctat tgccgttggt gggcttcgag aagcttcaat ggcgatcgcc gcgcctgttg
     3901 ctgccgcccg gaatggagtt ggatttcggc ggcatcggca aggaatatgc cgttgatcgc
     3961 gcttgcgagt tgctggcggc gcgcgacgca atcccattcc tcgtcaattt tggcggcgat
     4021 ctgcgcgcca acagaccccc atcgcacggt ccctggcagg tgggaatcga ggcgccagat
     4081 agcgatcggg aggcaagcat gttgctcgat ctggagtccg gcgcgctcgc cacgagcggc
     4141 gactcgcgtc gctatttgct aaaacacggc attcgctacg gccatatact gaatcccctc
     4201 accggttggc cagatccaga atcgccgcga tcaatcacgg tcgcggccag cagttgcacc
     4261 gaagcagggc tgctgtcgac ggtggcaatg ttgcatggcg ctggagcacg ggcgtacctg
     4321 gaagaacaag gcgcacgcta ctggctcgtc gaatgaacac agaccaaagg ctagcgttga
     4381 gcgcaatcag gcgcggcatt gacgctatac ttggcaatgc acgagtggcg caaagcagtc
     4441 ttcgggagct ccgacacgga atgaaatcca tgttttcagc ggtcgcgcgg ctggcaacgc
     4501 gcggcgtccg gtatgacaag acccgcaacg ccacgcggat gaagtggctg atgttcggat
     4561 gggcggtcgt cggaatcttc cctgtcgtcg gccgtacggc ggactcgctt ctcgatagta
     4621 cgctcgaaga tacgaagctg tacttcactt ctccgctgcg gtgggatgag caagattggc
     4681 tgtattttgg cggagccctg gccgctatcg gtgccgctca ctcgttcgat gaaagagttc
     4741 gagaccattt cgcaaccggc tcgagggcca tcctaaacgg aggcgcggac aagaacagcc
     4801 tgcgcgatgc cgctccgacg gtagcgatca tcgcgggcac cgggctgtat gcggcgttca
     4861 tcgatgatca tgatggatat cgcgaagcct ggtctttgat tgaagccggt gcgttcagcg
     4921 gcgccactgc agaagtgttg ggctacgcga caggccgaga gcgtcccgac gcgaccactt
     4981 cgccgaacga atggggcaag gggagcgact cgtttccgtc gctgcacacg accgttgcct
     5041 ttgcggtcgg gaccgtgttc gcagagtccg gcaatgacga gtaccgatgg attcgtcgta
     5101 tcatcggtta tggcgtggca ggtgccacgg gctacattcg agtcaatgag aatgtgcact
     5161 ggctatcgga cagcgtcgca ggcgcagcgc tcggcatcgc gaccgcgcga ttcgtcctca
     5221 atcgacaagg cgcacaggat aatcgtgccg cgttgcaatt ccagccgatc aagaatggct
     5281 ggatgatctc gtattcaatg cgtacccact gagcctcggc tttgggcgtc acacatattg
     5341 cgtcaaggca attccaccga gcgcgcccag gatgacaaca agaactggtg gagcgcgcca
     5401 cgcagtcagc aatacgaatc ctgtaagtgc gagcgcaaaa tcgccgctcg atcctacggc
     5461 gttgacccac agcaggccgt agagcgccgc gccgagcaag ccaacgacag ctgcgttgat
     5521 tcctcgcatg atggcgcgcg cgccgatgcg ctgccgcagg gtgtcccaga acggcaacgc
     5581 tcccaaaaga atcagaaacc cgggcagaaa aatcccggct atccccagcg ctgcgcctgc
     5641 gatcccgcgc ggcgagtggt tgcccacagc gccaagatag gccgcgaacg taaataaagg
     5701 tccgggcatc gcctgcgcgg cgccgtagcc cgccagaaag gaatcctgac tcacccagcc
     5761 gggcgtcacg aacgccttgc tcagcaacgg caatacgacg tggccgccac caaataccaa
     5821 cgcacccgag cgatagaagg cgtcaaagag ttcgaaccct cgatagcgca tgcccagcac
     5881 cgacaggacc gcgagcagga cgaagaacgc cgcgagagca acgatcccta cgcggcgcga
     5941 gacgggtatt ccgaggggcc gacttaccgt cggagccgac gcgcggcata gccaaaagcc
     6001 ggcgatgccg cccatgagaa ttacgccgat ttgagcggtc gaggagttgc cggataggac
     6061 gatgacggcg gcgacgcaag cgatcgaggc gcgctggcgg tccggacaaa gcgtcgctgc
     6121 cattccccac acggcctgcg cgacgatcgc gactgccacc agcttgagtc cgtgcaggat
     6181 ccctgagcca gtcgcactac cgagtgcgtt cgcgccgtaa gcgaatgccg tcatgagtac
     6241 cgcggatggc agcgtaaagc ccgcccaggc cgccagggcg ccccaatacc cagcgcgaat
     6301 caatccgagc gaaaatccga cctgactgct ggcgggccct ggcaggaact gacagagcgc
     6361 tacaagatcg gtgtacgtgg cgtcatcgac ccaacggcgg cgaacgacga tttcctcgcg
     6421 gaaataaccg atgtgcgcaa ccggaccgcc gaacgatgtg agtcccagct tgagaaagct
     6481 gagaaatacc tcgagagccg atccctcatg catacgtata gcgccatgac gccgaaattc
     6541 attgccgatt atgccggata ctgccggcat cttgcgcatc gagcgcgccg agccgggaaa
     6601 ctgcggcctg cgcgcgcaag accgcatgcg cgcgcgtcag taaaatccta cgccgctgcg
     6661 cgacggccgc cgaatcagca gcgcgtcttt gccggctcgg tcaatcaggc cgatgtcgcg
     6721 ccttcgtcct acagaaagtg gttctcgttc cgcgttaaat ggctggggcg ggcgctcgtg
     6781 gtgaacgtcc cattcctcga gaggcggttg ttcgtgaaac actcgaccct acatggcacc
     6841 tggccagact tgtcggtcct tcgggcatga cggtgcacgc catcaaagtg ctgctggtgg
     6901 aggattcgtc ggttctcgca gaacgattgg cggagaccat cactgaattt ccggagatcg
     6961 acttgatcaa ttccgtggat tccgaatcaa gtgcgctcgc cgaaatccgc aaacgtcgaa
     7021 tcgacgtcgt ggtgctcgac ttgcaactga aggaaggatc cggcttcggc attctgcgtg
     7081 ccgtggcagg gatgcagcag aagccgtgcg tcgtcgtact gacgaatcat gactcatctg
     7141 cttatgagcg ggaggcggtg tctttggggg cgcggtattt cttggacaag gcgcgcgatt
     7201 ttcaccgcct gcccgacata ttgcgggaga ttgtcctctg cggagtcacc acctgacctc
     7261 gatgggcttg tcgttgacga tcatcgccaa tatttcggat gcagtgctca tcgatcgcct
     7321 gcagcgagcc gcatacgact acttcgggcc gaacagcaac cccgacaacg gcttggtcgc
     7381 ggacacctcg cgcgcaacat cccccagcag tattgcggta gtcggtttcg gattgtcgtg
     7441 ctatccggtt gccgtggaac gcgggtggat cgggcgcgac tcggcggcgg cccgcgtgct
     7501 ggcgacgttg cggttctttt gggatagcga acaaagcgaa cagcgcaacg ccacaggcta
     7561 tcgaggcttc tactatcact tcttggacat gcagagcggc cggcgcgcct gggagtgcga
     7621 attgtcgctg atcgacacag cgctgttgct ggccggcgtg ctcacggcgg ccctgtactt
     7681 cgacggggaa gattccgaag cccagatccg cgaactcgcg cgcgcgctgt atgaacgggt
     7741 ggactggacg tgggcgctca atggcgggga gacactggca caaggctggc gacccgaaac
     7801 aggatttctg cactacggct gggaagggta taacgaagcg acgttgttgt atttgctcgg
     7861 attggcatcg ccgacccatc cgctgcctgc ggccagcttt gcgagctgga ccttcaccta
     7921 tcaatgggag aatttgctcg gcatcgacac gctgtactcg ggaccgttgt ttacgcacct
     7981 attctcgcag gcctgggtcg attttagggg aattcgcgat cccttcatgc gccaagcggg
     8041 cagcgattat tttgcgaaca cccagagtgc gattgcaatt cagcgcgagt acgcggcgcg
     8101 caatccgcgg ggcttcgtcg gctacagccg caatctgtgg ggcatcacgg ccggcgatgg
     8161 acccgcaaac gcggacaatg tgcggaggtc acgcgatcgc cgctattttg gctacatggc
     8221 gcgcggcgtg ccctttggtc cggacgacgg cacgctcgcc ccgtgggcga tgctggcgag
     8281 cctgccgttc aatccggacg cggcgctcga ggggacccgt cacgtgttgc aacgccatcc
     8341 cgaggtgtgt ccgcaagacc ggtttgtgag cggcttcaac ccgagtcgca taaccaagaa
     8401 aaagccgtgg ctgtcgcagg gctggtacgg actcgaccag ggcctgctgg tgatgatgct
     8461 cgagaatcac cgctcggggc tcatttggaa cctcacccga gcttcgccag tgtttcggtc
     8521 gggactgcaa cgggccggat tcaaaggcgg atggttggac tcgtgacgca gagggttgat
     8581 gttgcgtatc ttgagcgggt tcgcccgccg gtgtggcaca acccggcacc gagcggccac
     8641 tatgatctgg tgatcttggg cggtggcccg gccggtctga ccgctgcgag ggtcgcccgg
     8701 tcgctcggcc tctccgtcgc gctggtcgaa cgcgcgcggc tcggcggcaa ttcgctgaat
     8761 gccggatcca tcccctccaa ggccttggtg cgaagcggcc gtgctttcgc agccatgctc
     8821 aacggccggc gcttcggcgc ccccgatgcg ggtgaaccca tggctgactt ttccgcggtc
     8881 atgcggcgca tgcatgcggt tcgaagccgc atcgccgaat cgtgctccgc gctgcggctc
     8941 accgcgatgg gcgtcgatgt gttcttcggc cacgccgagt tcgcgcaaga ccggtgcatg
     9001 caggtcggag ccgcagcatt gcattttaga aaggccctga ttgcggccgg cgcacgtcct
     9061 cgcgcagccg caattcccaa cctcgaggcg gtggggtacc tcaccagcaa cagtttcttc
     9121 gagatcgaac ggctgccgcg gcgcttggcg gtgatcggcg gcggccccct gggttgtgag
     9181 atggcgcagg cttttgcgca catgggatcg agggtcacga ttctgcaaga tgagccgaaa
     9241 ttcctgcccc gcgaggaacg cgatgcggcc gaaatcttgt cgctgtcgct cgcgcgctcc
     9301 ggcgtggaga cgcacctcaa cacaacgatc gtgggcgcgc gcgcggcaaa tggcgaaaag
     9361 ctgatcgatt tcgagaatac gggcgtcagc cgcacgctgg ccgtcgatga gatattggtg
     9421 agcatcggcc gcgtcgccaa ttccgatgag ttgcggctcg cggccgccgg catcgccacg
     9481 gcggccgacg gacgcataca aatcgacgat ttcctcacca ccagcaaccc ggatgtgtac
     9541 gccgccggcg atgtgtgcat ggctcacaag aatacgaatg tggcgcaagc ttccgcggag
     9601 atggccgtgc gcaatgcatt tgccatggcc gagcaacgtc acagctcgat cgtggctccg
     9661 cgctgcacct attgcgatcc ggaaatcgcg cacattggga tgcacgtttg ggatgccgca
     9721 aggcagggca tcgcggtgaa gacctacacc atcatgatgc aggacgtcga gcgcgcgatc
     9781 atcgatgggc gggtggacgg tttcgtcaag attcacctgc gagcgggcag cgatgaaatc
     9841 atcggcgcct ccatcgtcgc ctcgcgtgcc agcgaaatga tcaacgaagt ggcggtgatc
     9901 atgcatgcca aacttgggat gcgtgcgctc gcgggaattc tgcatgccta ccccgcgcag
     9961 tcagatgcga ttcgcctcgc cgcatgcgcc tacatgaact ccctgaacca tgcagttcgc
    10021 tagcgccctg cgaaacgccg gtgcgccagg ccgcggcggc gcttccgggc cgggcgagat
    10081 tttgagcgtc gaacgcctgg aggtctatgc ggagaattta gcgaggacgc aagaggtatt
    10141 tccaacccgc aaagccggca tctcgttcag cggtcgcctc aagtcaaacg cgatcgcgtt
    10201 gaacggcgcg tttcaggcac tcgtcgtcgg tattcgcaag ggtgaaacgg tgacacccgc
    10261 ggccgcctgg ttggtcgaca atttctatat catcgacgag cacctcaagg cgattcgccg
    10321 cgacttgcca cccgggtatt acaagcaact ccccaagctt gcaaatggaa ctctgcgcgg
    10381 ctacccgcgc gtgtatggca tcgccgcggg cctggtggat cacaccgaca atcgcttcga
    10441 actcgatact ctgtatcgct acgttcaggc ctaccagcgc attcaaccgc tgacgatcgg
    10501 cgagttgtgg gccatcgcga ttacgttgcg cgtgatactc atcgataatc tgcgagacct
    10561 ggcggtcggc atcgtttcgc gcctgtccga gcgcgagcag gccgatgcgt tggcggatca
    10621 atggctggca gatgactcga ccgcggcgca gagccgtttc ttgccggcaa cgcgcttgac
    10681 gccgcccttt gccgcgcaat tgtttcgccg gctgcgagat cacgatccca gcaccacgcc
    10741 cgcgctcgca tggctgcatc agacgcttgc cgcccagaat tggagcgccg atgaaatcgt
    10801 acacagcgaa catcagcgac aaggggccgc gaatgtcagc gtccgcaata tcatcaccag
    10861 cctgcgcttg atatcgtcgg tcgattgggc tcacttcttc gaaagcgtca gtccggtcga
    10921 tgaactgctg cgcacgcaat cggcgtacgg gagcttcgat ttcctgacac gtgacctgta
    10981 tcgacatgcg atcgaagagt tggccagacg ctgcaatcac acggaactcg aagtggcgcg
    11041 gcgcgcgatc caggcggccg aacgggccgc aggcggcgcc gcgacgcagg gcggagccgg
    11101 atcggcgcca cgggagccgg gatactacct cgtctgcgcc ggacgtccgc aattcgagcg
    11161 cgaactcggc tataaacgcg cgtttcgcga gcgcgtcgcg cgcttcaccc gcagggccgg
    11221 cgtgctcggc tatgttggaa cgatattcct gttgagcagc gcattcagcc tcgccctcgt
    11281 atgggaggtc gggctctctg gccgcgacat cggagtaagc gtggcgctga caggcctcgc
    11341 attgctcgcg gccaccgaca tcggcattgc cattgtcaat ctctgggtag ccgcattttg
    11401 ccccgcgcgc gcgttgcccg cgctggaact cgaggatggc gtgccatccg aattgcggac
    11461 cttgctggtg ataccgattc ttctgacgac gctcgcggaa ctcgaggaga gcattcgcaa
    11521 tttggaagtg cattatcttg cgactcacga gggcgacatt cgattcgcgc tgctgtccga
    11581 ttggtgcgac tcgccggaga aaatcgcgcc ggccgatgag gaattgctgg cggcggccga
    11641 ggccggtatt gcgcgcttga atcgcaccca tgggaccccg gaagacggcg ctcgtttcct
    11701 gctgctgcat cgccggcgca tgtggaacgc atcggaacag cgctggatgg gctgggaacg
    11761 aaagcgcggc aagctgcatg agctcaactt actgctgcgc ggctcgcaag acacgagttt
    11821 tctcggcagc gccgcgcgac ccctggccgt gcccgccggc gtccgctatg tgatcagtct
    11881 cgatgccgat acgcgtttgc cgcgcggcgc agcgcgggcg ctcatcggca aaatggccca
    11941 tcccttgaat cagccggtca tcgatcccgt ccgccggcgc gtgacatcgg gttacgggat
    12001 cttgcaaccg cgggtgacac cgtcgcttcc cgcagggtca gacgcatcct tttttcagcg
    12061 tgtctcctcg ggtccgagcg gcatggatcc gtacgcgttc gcggtttccg acgtgtacca
    12121 ggacatgttc ggcgaaggct cctacagcgg caaaggcatc tacgatgtga ccgccttcga
    12181 ggcagccctc gagaatcggg tacccgagaa ccgtcttctc agccacgacc tgttcgaggg
    12241 cagctacgcg cgctgcggac tcgcgtccga tgtcgaggtc atcgaggagt atccgtcgcg
    12301 atacgatgtc gcgagcgcgc ggcagcatcg ctggacgcgc ggcgattggc agttgttgcc
    12361 gtgggtgctg cacgacggct cgctgacggc gctggctcgc tggaagatga tcgataacct
    12421 gcgtcgctca ctgatcgcgc cggcggtgtt tctcggattg gcgctcagct gggcggccgc
    12481 tgcagcggct gcctggaccg cattctttct cgcgaccctc agcttgccgg tgctcttgcc
    12541 gttggtggtc ggctggttgg cgcgagtcga gatcggtcgg agcgtgttca ggccggcgcg
    12601 caaggaggcg atccaggcgc tgtcactgat cgccttacgc ctgatatttc tcgcggatca
    12661 agcctgctcg gcgggggatg cgattgttcg caccctgtac cggctcgcga tctcgcaccg
    12721 gcatctgctg gaatggacga caagcgccca agcgaaaagg cgcggcttca cagccacgtc
    12781 gacgctgttt ggagcgatgg cggcgggctg gttgttgagc gcagcgacgg cgcttgtgct
    12841 cattgccgcc cgcggccgcg ttccctggga gtcggtgccg tggctcgcgg cgtggttcgc
    12901 cgctccactg atcgcacggc gcatcagcgt gcgcgcggcg aagcccattg ccgaagcatt
    12961 gacgcacgcg gagacgcgcg agctgcgcga tatggcgaaa caaacctggg gctacttcga
    13021 taccttcgtg agcgcgcagc agaatcactt gccgccggat aacttccaag aaataccgag
    13081 cgccgtaata gcgcatcgaa cctcgccgac gaatgtgggc ctgtatctgt tgtcggtcg
//
LOCUS       DASWZT010000336         1668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_70386, whole genome shotgun sequence.
ACCESSION   DASWZT010000336 DASWZT010000000
VERSION     DASWZT010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70386"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VGE92_09230"
     CDS             complement(<1..248)
                     /locus_tag="VGE92_09230"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY1314049.1"
                     /translation="MNDLSSNPANLTFEFGGARLRYRDSGRGPAVLFLHGWTLDLDMW
                     EFQAADLAARYRIVRLDRRGFGLSSGVPSMVQDVRDVEA"
     gene            complement(357..1163)
                     /locus_tag="VGE92_09235"
     CDS             complement(357..1163)
                     /locus_tag="VGE92_09235"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY1314050.1"
                     /translation="MMRTKPAQRREAMGGLAKGLAVIRAFARDRATLSLSDVAALTDM
                     PAATARRCLLTLEELGYVTRNGRQFVLRPKVLELGAAYLESMNIETVTKNHLEELAGS
                     TGDSAALCVLDGTEIVYIARASIRTLIRLEAHVGSRFPAHATSTGRALLAGLTAERLQ
                     RYFDTATFEALTDRTVVEPARLKQLIEDCRRTGYAAVEDELAYGVVALAVPVFDQYGR
                     VVAALNSSSHSRRISKAKLVRERFSMLQTVSRQISADLASLPGLSMSATV"
     gene            1307..>1668
                     /locus_tag="VGE92_09240"
     CDS             1307..>1668
                     /locus_tag="VGE92_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314051.1"
                     /translation="MKIGSQLHKQSAISTSNETTILVRGHDLTHELIGHIGFTEHFWL
                     LLTGALPTPAQRRILDATLVAIAEHGLVPSVQAARMTLAAAPEALQGAVAAGILGCGS
                     VVLGSSDAAGKFFASIAAG"
BASE COUNT          348 a          513 c          503 g          304 t
ORIGIN      
        1 gcctccacat cgcgcacgtc ctgaaccatg gaagggacac cggatgacaa gccgaagccg
       61 cgccgatcga gtcgcacgat tcggtatctc gcggcgagat ccgccgcttg aaattcccac
      121 atatcgagat cgagagtcca accgtgaaga aacaacacgg ccggaccacg ccccgaatcg
      181 cggtaacgca gccgcgcgcc gccgaactcg aaagtgagat tcgccgggtt cgaactcaga
      241 tcattcatgg ttggcaagtc tgaaatcacc gccatcctgt ttttcgtata gcgaaaaaag
      301 gttcgcaaac aggctacgcg gaccgcctat tgaacgtcaa ggcggcgagc cgccactcaa
      361 acggtggcgc tcatcgacaa acccggcagg ctcgcaagat cagcgctgat ttgccgactg
      421 accgtctgca gcatgctgaa gcgctcgcgg accaacttgg ccttgctgat ccggcgcgag
      481 tggctggagc tattcaaggc agcgaccacc cggccatatt gatcgaaaac cggcacggcg
      541 agtgccacca cgccatacgc gagttcatcc tcgacggcgg cataaccggt gcgtcgacag
      601 tcctcgataa gttgcttgag gcgcgcgggc tcgaccacgg ttcgatcggt caatgcttcg
      661 aacgttgccg tgtcgaaata gcgttgcagg cgctcggccg tgaggcctgc cagcagggca
      721 cgccccgtgg atgtggcatg cgcggggaag cggctgccga catgcgcttc gagcctaatc
      781 agtgtgcgaa tcgaggcgcg cgcgatatac acaatttcgg tgccgtcgag cacgcacagc
      841 gccgccgagt caccggtact acccgcaagt tcttccaagt gattcttggt caccgtttcg
      901 atattcatcg actcgagata ggcggcgccc aactcgagaa ctttcggacg cagcacaaat
      961 tgccggccgt tgcgggtgac atagcccaat tcctcgaggg tgagcaggca gcgccgcgca
     1021 gtcgccgcgg gcatgtcggt gagggcggcg acatcactca agctcaatgt cgcccgatcg
     1081 cgcgcgaagg cgcgtatcac cgcaagtccc ttcgcgagtc cgcccatggc ctcgcgccgc
     1141 tgagctggct tggtgcgcat catgcgcggc agtttatttc gatatgcgaa aaggaggcaa
     1201 cagaattcgc gactctgttg acgctggcga aaatcaagct cagcatattc gttatacgaa
     1261 tcgcttttcg gtatgcgaaa aaacccaaag ctgaagagag cgcaatgtga agatcggatc
     1321 acaactccac aagcaatccg cgatttcaac ctccaacgag accacgatcc tggtgcgcgg
     1381 ccacgatctc acacacgaat tgatcgggca tatcgggttc acggagcatt tttggctgct
     1441 cctcaccggt gcattgccga cgccggcgca gcggcgcatc ttggacgcga cgctggttgc
     1501 gatcgccgag catggcctgg ttccgagtgt gcaggccgcg cggatgacgt tggcggccgc
     1561 gcccgaggcg ctgcaaggcg ccgtggctgc gggcattctc ggatgcggct ccgtggtcct
     1621 cggctcctcc gatgccgcgg gcaagttctt cgcgtcgatt gcggcggg
//
LOCUS       DASWZT010000337         6442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_22080, whole genome shotgun sequence.
ACCESSION   DASWZT010000337 DASWZT010000000
VERSION     DASWZT010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6442
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22080"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VGE92_09245"
     CDS             complement(<1..601)
                     /locus_tag="VGE92_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314052.1"
                     /translation="MPTFLKLMRQTKLWSLSALLLAACGGAGSHGGASPTAPLTPARG
                     TLLESPASLLSTVKTSDVLLALNLSANANLLSVSPPLICDILFYHIVYETVGGAGEPT
                     TASAVLMVPTGLGASCTGARPIVLYAHGTTTDRAFNMANLQNTETLSLAALFASQGYI
                     VVAPSYAGYDTSTLPYHPYLIADQQSKDMIDALTAARTTL"
     gene            complement(706..3048)
                     /gene="pbpC"
                     /locus_tag="VGE92_09250"
     CDS             complement(706..3048)
                     /gene="pbpC"
                     /locus_tag="VGE92_09250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HEY1314053.1"
                     /translation="MRLSRPAIRRLLGSLLTILLLGTLGVGLARSLSHVPLSSFAPQS
                     TAVSAQSGELLRLTLAADGQYRLWVDLERMPSRLPAAVLLYEDRWFYLHPGVNPVALA
                     RAMFEAGVGRRRVGASTITMQLARRMYRLNTRDPRGKLRQMALALWLEARYSKHDILE
                     AYLNLAPYGRNVEGVGAASLVYFATPAAHLSVPQVMTLAVIPQNPRERRLRSGDANSR
                     KLADGLEEARARLWARWVKAVPDDARFSAAVAAPISARPPEQLPFAAPHFTDALLRSA
                     AGEVQSTLDLARQRTVERILTKYIEGESQLGIRNAGALLLEVGGGVATVRAYVGSADY
                     RDARIDGQVNAVTAKRSPGSALKPFIYGLALDQGLLHPRTILKDAPTAFGPFSPENFD
                     GRFMGPIAAEDALIRSRNVPAVAVASQLSRPTLYEFLQVGGVSRLKSERHYGLALALG
                     GGEVSMEELGRLYALLLNGGRLPAIRSILSQEPMTGGVRLLSEESSFIVLDMLSKNPR
                     PDTGAPASPAVAWKTGTSWGFHDAWSVAVFGRYVLLVWIGNFDNTGNPAFVGVQAAAP
                     LLFRIIDALRAQGLAAGDLVRRFPASVARIEVCAASGDLPNPACPQTVSTWYIPGKSP
                     IRLSTLHRYVYFDAANHRVCGPGPEVRQEVYEFWTSDMLRLFREAGMPRRTPPADPDC
                     GNGSEPGGSDSEALHIVSPLRGLVYTERALHPAPLALRADVGAATHAVYWFAGNAFIG
                     RAPAGETLPWLPQQSGRYTLRAVDDRGGADTRELEVELVP"
     gene            complement(3064..>6442)
                     /locus_tag="VGE92_09255"
     CDS             complement(3064..>6442)
                     /locus_tag="VGE92_09255"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEY1314054.1"
                     /translation="DLQATVSVANLFGTAAQQRRVQAELTLTPAFPAFRTLPEFRFYD
                     PLRAKEGYVDTLAEVKTDAAGNASFDLGLQKYARATYRVHVLARAFEPAGGRSVTADT
                     AVLVSDLPFLVGFKADGDLAYIAKSSKRVVSWIAIDPTVKRTAALGLTLRRVETTFVS
                     VLAKQPNGTYQYESRKKDITLSEEAFALPAAGLEQTLATDSPGTFAYIIRDAAGLELN
                     RVEYSVVGQANLTRSLERNAELQIQLDKQEYAPGEEIELSIKAPYTGAGLITIERDRV
                     FATRWFKTDTTASVQRIRLPADFEGNGYISVQFTRDPGSDEIFASPLSFGVVPFATSL
                     AARRNALTLTVPTLVKPGTLMHIDLQAAHATRAVVFAVDAGILQVARYATPDPLGEFF
                     KKRALEVTTSQILDLLLPDFKRVLGAAAPGGDGEAALRRNLNPFKRKHAAPAVYWSGI
                     VDVEQGREFTWTVPDSFNGSLQVFAVAVNETTIGVAQAQTTVRGDFVLTPNMPVAVAP
                     GDEFEVSVGIANGVAGSGSGAAVSVSAELPPQLEALGAARQTVSVAEHGEAVALFRFR
                     AKALLGAAPVRFTAAWQEHAAHLVEEVSVRPASPYVTEVLAGYFGSAIDVPIGRSLSP
                     DHRKLTVGVSAVPLVLAGGLADYLDDYPHLCTEQLVSRGFATLVLARRPELARSEAAN
                     GRRPADAVAALVAVLRSRQNAEGGFGLWAASITTDEFASVYAMHWIIDAREQGQRIPL
                     DLYQKGQVWLQRYAVSPVADHELAGLAGLRNRAHATYLLAREGLVTTPIGSNLVETLD
                     ARYPKLWRSDAAAAYLAAVYQLQHQEREAKPLIEHLAAGLGSSSGTPFAYFDDDTVRG
                     AELLYLLSRHFPERARALKPQVLQALVAPLARGEYNTLSAAFLVMAFDAYATNVPSET
                     LTQFTGTQTAAGGAASPLELRGQLILRGTYDGDTAQLHFANDTGMAGFFAVTNAGFDR
                     NPPVEALREGLEIQRDYLNAQGEPVLSVQSGEEVTVRLRLRSIDRTFVTDVAVTDLLP
                     GGFEPVLNNPPSAERAWVDPLGSGAWRPQFADVREDRVVLYGMLSKDMAEYSYRIVAT
                     NAGRFTVPPSYAESMYDRKLRARAGAGHVTVVRPAAH"
BASE COUNT         1137 a         2155 c         2138 g         1012 t
ORIGIN      
        1 gcagggtggt gcgcgcggcc gtcaaggcat caatcatgtc cttcgattgc tgatcggcga
       61 tcaggtacgg atgatacgga agcgtcgaag tgtcatagcc cgcatagctc ggtgcaacca
      121 cgatgtaacc ttgcgacgca aacagcgccg cgagggacag ggtttcggta ttttgcagat
      181 tcgccatgtt gaaggcgcga tccgtggtcg tgccgtgggc atagagcacg atcgggcgtg
      241 caccggtgca gctcgcgccc aaccccgtgg gcaccatcag caccgccgaa gcagtcgtcg
      301 gttcgccggc gccgcccacg gtctcgtaaa cgatgtgata gaaaagaatg tcgcaaatca
      361 gcggtggact caccgacagc aaattcgcat tcgcgctgag attgagtgcg agcagcacat
      421 ccgaggtctt cacggtggag agcaaactcg cggggctttc caggagcgtg ccgcgggcgg
      481 gagtgagggg tgccgtcggg gacgcgccgc catgactgcc cgcaccaccg cacgcggcca
      541 gcagcagcgc cgataagctc cagagcttgg tctgccgcat cagttttaaa aatgtgggca
      601 taccccaacg acccggttcg cggggggaag ttcgcaacct ccccgtgaca gaaccttcat
      661 cgacgcgaaa tcgcccattc gaggctcgga tggccacatc aagtgctacg gcaccagctc
      721 aacctcgagt tcgcgcgtat ccgcgccgcc gcgatcgtcc accgcgcgca gcgtgtaacg
      781 cccggattgc tgcggcagcc acggcaaagt ttctcccgcc ggcgcgcggc cgatgaaggc
      841 gttgcccgcg aaccagtaca cggcgtgcgt ggctgcgccg acatccgcgc gcagcgcgag
      901 cggcgccggg tgcagagcgc gttcggtgta gaccaagccg cgcagcggcg acacgatatg
      961 cagcgcctcg ctgtcgctgc cgcccggttc ggatccgttc ccgcagtccg gatcggcggg
     1021 cggcgtacgg cgcggcatgc cggcttcgcg aaacaaccgc aacatgtcgg atgtccagaa
     1081 ttcgtacact tcctggcgca cctcgggacc cggaccgcag acccgatggt tggccgcatc
     1141 gaagtacaca taacgatgca gcgtcgatag gcgaatcggc gacttccccg ggatatacca
     1201 agtgctcacc gtctgcgggc atgccgggtt cggcagatcg ccgctcgccg cgcacacctc
     1261 gatgcgcgcc acactggcgg gaaatcgccg caccaggtcg ccggcggcga gcccctgggc
     1321 gcgcagtgca tcgatgatgc gaaacagcag cggcgccgcc gcctgtacgc cgacgaaggc
     1381 gggattaccc gtgttgtcga aattgccgat ccacaccagc agcacgtagc ggccgaacac
     1441 cgcgaccgac caggcgtcgt ggaatcccca tgacgtgccg gtcttccagg cgaccgcggg
     1501 cgaggccggc gcgccggtgt cgggtcgcgg attcttgctc agcatgtcga gcacgatgaa
     1561 gcttgattct tcggacagca ggcgtacccc gcccgtcatc ggctcctgac tcaggatcga
     1621 gcgaatggcc gggagccggc cgccgttcag cagcaaggca tagagccgcc ccagttcttc
     1681 catcgacact tcgccgccgc ccagggcgag ggccagcccg taatggcgtt cgctcttcaa
     1741 acgcgaaacg ccgccgacct gcaaaaactc gtacagcgtc ggccgcgaca actgactcgc
     1801 caccgcgact gcaggcacgt tgcggctgcg gatgagcgca tcttcggcgg cgatgggacc
     1861 catgaagcgg ccgtcgaaat tctcgggact gaacgggccg aacgcggtcg gtgcgtcctt
     1921 gaggatggtg cgcggatgca gcagtccctg gtcgagcgcg agtccgtaga taaagggctt
     1981 gagcgccgaa cccggcgagc gctttgccgt cacggcattc acctggccat cgatgcgcgc
     2041 atcgcgatag tcggccgagc ccacataggc gcgcaccgtc gctacgccgc cgcccacctc
     2101 gagcagcaaa gcgcccgcat tgcggatgcc gagctgcgac tcaccctcga tgtacttcgt
     2161 caggatccgc tcgaccgtgc gctgccgcgc aagatcgagc gtgctctgca cttcgcccgc
     2221 ggcgcttcgc agcagcgcgt cggtgaaatg cggcgcggca aacggcagtt gttcgggtgg
     2281 tcgggcgctg atcggcgcgg cgacagcggc gctgaagcgt gcgtcatcgg gcacggcctt
     2341 cacccagcgc gcccagagtc gcgcgcgcgc ctcctcgagc ccatccgcaa gctttcgcga
     2401 attcgcatcg ccggatcgca aacgccgctc gcgtggattc tgcggaatca cggcaagggt
     2461 catcacctgc ggcaccgaca gatgcgccgc cggcgtcgca aagtacacca ggctcgccgc
     2521 gccgacgccc tcgacgttgc gtccgtatgg cgcaagattc agatacgcct ccaggatgtc
     2581 gtgcttgctg tagcgcgcct cgagccacaa cgcgagcgcc atttggcgca gcttcccccg
     2641 ggggtcgcgc gtgttgagcc ggtacatgcg acgcgccagc tgcatcgtga tggtcgaggc
     2701 gccgacacgc cggcggccga cgccggcctc gaacatcgcc cgggcgagtg ccacgggatt
     2761 gacccccggg tgcaggtaga accagcggtc ctcgtacagc agcaccgccg ccggcagccg
     2821 cgacggcatg cgctcgagat ccacccacag ccgatactgg ccgtcggcgg cgagcgtgag
     2881 gcgcagcagc tctccggact gcgcactgac ggcggtcgat tgcggcgcga acgaggaaag
     2941 cggcacgtgc gataggctgc gcgccaaccc cacgccgagc gtgccgagca acaggatggt
     3001 gagcaagctc ccgagcagtc ggcggatggc aggacgtgac agccgcacgc gcgcgggctc
     3061 cgctcaatgc gccgcgggcc gcacgaccgt cacatggccg gcacccgcgc gcgcccgcaa
     3121 cttgcggtcg tacatggact cggcataact cggcggcacc gtgaaacggc cggcgttggt
     3181 cgcgacgatg cggtagctgt attcagccat gtccttcgac agcatcccgt acagcaccac
     3241 gcggtcctcg cgcacgtcgg cgaactgcgg ccgccaggcg ccgctgccca gtggatcgac
     3301 ccacgcccgc tcggctgagg gcggattgtt caacacgggc tcgaagccgc ccggcaacag
     3361 gtcggtgacc gcgacatcgg tgacaaacgt gcgatcgatc gagcgcaagc gcagccgcac
     3421 ggtcacttcc tcgccgctct gcacgcttaa gacgggttcg ccttgggcgt tcagatagtc
     3481 gcgctggatc tcgagaccct cgcgcagtgc ctcgacgggc ggattgcggt cgaagccggc
     3541 attggtgacg gcaaagaagc cggccatgcc ggtatcgttc gcgaaatgca gctgcgcggt
     3601 gtcgccgtcg taggtgccgc gcagtatcaa ctggccgcga agctcgagcg gactcgccgc
     3661 gccgcccgcc gcggtctgcg tgccggtgaa ctgggtcaac gtctcgctcg gcacgttcgt
     3721 cgcataggca tcgaatgcca tcacgaggaa ggccgcggac aaggtgttgt actcgccgcg
     3781 cgccaacggg gccaccagcg cttgcagaac ctgcggcttg agtgcgcggg cgcgctccgg
     3841 gaaatgccgc gacagcaggt acagcagctc ggcgccgcgg accgtgtcat cgtcgaagta
     3901 ggcgaagggc gtgccgctcg aggaaccgag ccccgccgcg aggtgctcga tgagcggctt
     3961 cgcttcccgc tcctgatgct gcagctgata caccgcggcc agataggcgg cggccgcatc
     4021 gctgcgccag agcttcggat agcgcgcatc gagcgtctcg accaagttcg agccgatcgg
     4081 cgtcgtcacc aacccctcgc gcgccagcag gtacgtcgca tgcgcgcggt tgcgcaaacc
     4141 cgccagaccc gcgagttcat ggtccgcgac cgggctcacc gcatagcgct gcagccacac
     4201 ctgtcccttc tgatacaaat cgagcgggat cctctgaccc tgttcgcgcg catcgatgat
     4261 ccagtgcatg gcgtacaccg aggcgaactc atcggtcgtg atcgacgcgg cccagagtcc
     4321 aaagccgccc tcggcatttt gccggctgcg cagcaccgcg accagcgccg ccacggcatc
     4381 cgcgggccgg cgtccgttcg cagcctcgct gcgggcgagt tccggccggc gcgcgagcac
     4441 cagcgtggcg aagccgcggc tcaccagctg ttcggtgcaa aggtgcgggt aatcgtcgag
     4501 atagtcggcg aggccgccgg cgagcaccaa tggcaccgcc gagacgccga cggtgagctt
     4561 gcggtgatcg ggggagagcg agcggccgat gggcacgtcg atggcgctgc caaagtagcc
     4621 ggcgagaacc tccgtgacat acggcgaggc agggcgcacg ctcacctcct cgacgagatg
     4681 cgcggcatgc tcctgccacg cggcggtgaa gcgcaccggc gcggccccga gcagcgcctt
     4741 ggcgcgaaag cggaacagcg ccaccgcctc gccgtgctcc gcgacgctca cagtctggcg
     4801 agcggcgccg agcgcttcga gctgcggcgg caactccgca ctcaccgaca ccgcggcacc
     4861 gctgcccgaa cccgcgaccc cattggcgat gccgacactc acctcgaatt catcgccggg
     4921 agccaccgcg accggcatgt tcggcgtcag tacgaaatcg ccgcgcaccg tggtctgcgc
     4981 ttgtgcgacg ccgatcgtgg tctcattcac tgccaccgca aacacctgca acgagccgtt
     5041 gaaagagtcg ggcaccgtcc aggtgaactc gcgtccttgc tcgacatcca cgatgccgga
     5101 ccaatagacc gcgggcgcgg cgtgtttgcg cttgaacggg ttgagattgc ggcgcagcgc
     5161 cgcctcgccg tcgccgccgg gggccgcggc gccgagcacc cgtttgaaat ccggcagtag
     5221 cagatcgaga atttgcgagg tcgtgacttc gagcgcgcgc ttcttgaaga actcgcccaa
     5281 gggatcgggc gttgcgtagc gcgccacttg caaaatgcct gcatcgaccg caaacaccac
     5341 ggcgcgggtt gcgtgcgcgg cttgcagatc gatgtgcatc agcgtgccag gtttgaccag
     5401 agtgggtacc gtgagcgtca aggcgttgcg gcgcgcggcg agactggtcg cgaacggcac
     5461 cacgccgaag ctcaaggggc ttgcgaaaat ctcatccgat cccgggtcgc gggtgaactg
     5521 cacgctgata tagccgttac cctcgaaatc cgcgggaagg cggattcttt gcaccgaagc
     5581 cgtggtgtcg gtcttgaacc agcgggtcgc gaatacccga tcgcgctcga tcgtgatgag
     5641 gccggcgccg gtgtagggcg ccttgatgct gagctcgatt tcctcgcccg gcgcatactc
     5701 ctgcttgtcg agttgaattt gtagctccgc attgcgctcc aaggagcgtg tcaagttcgc
     5761 ctggccgacc acgctgtact cgacgcgatt gagctcgagc cccgcggcat cgcggatgat
     5821 gtaggcgaag gtaccgggac tgtcggtggc gagcgtctgc tctaaacccg cggccggcag
     5881 ggcaaacgcc tcctcggaga gcgtgatgtc cttcttgcga gactcgtatt ggtacgtgcc
     5941 gttcggctgt ttggcgagca ccgaaacgaa ggtggtttcg actcgtctta aggtcagccc
     6001 cagggcggcg gtgcgcttga cggtcggatc gatggcaatc catgacacca cgcgtttgct
     6061 cgatttcgcg atgtacgcga ggtcgccgtc ggccttgaag ccgaccagga acggcaggtc
     6121 ggagacgagt accgccgtgt cggccgtcac cgagcgccca cccgcgggtt cgaaggcgcg
     6181 cgcgagtacg tgtacgcggt aggttgcccg ggcgtatttc tgcagaccca aatcgaagct
     6241 ggcattgccg gccgcatcgg tcttaacttc ggcgagcgtg tcgacgtagc cttccttggc
     6301 acgcagcgga tcgtagaacc gaaactcggg cagcgtgcgg aacgcgggga atgccggggt
     6361 gagcgtgagc tccgcctgca ctcgccgctg ctgcgccgct gtgccgaaca ggttcgcgac
     6421 gctcaccgtc gcctgcaaat cc
//
LOCUS       DASWZT010000338         2655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_2687, whole genome shotgun sequence.
ACCESSION   DASWZT010000338 DASWZT010000000
VERSION     DASWZT010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2687"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1345
                     /locus_tag="VGE92_09260"
     CDS             173..1345
                     /locus_tag="VGE92_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HEY1314055.1"
                     /translation="MIDTSAIRERFAAVSRDLNERSRRLLAAAEAKTAGHGGIAAASR
                     ATGIARSTIGRGLKDLAAPDGLSGDIRRPGGGCPTLVDKDPALLDDLRRLVEPATMGD
                     PTRPLMWVSKSHAKLAAALRDMGHKIADSSIPKLLGLLDYRRQVNRKTLEGSHNPDRN
                     AQFEHINAAVTATQAMGQPVISIDTKKKELIGAYKNGGSDYRPEGCPDKVNVHDFIDK
                     DLGKAIPYGVYDIAANAGCVSVGISNDTAQFAVNSIRHWLDTMGRERYPAMQQLMITA
                     DGGGSNGSRVRLFKIELQRLADETGLTVCVCHYPPGTSKWNKIEHRLFCHVTQTWRGK
                     PLVSRETVVELIAATTTRTGLIVRCALDTRDYPKGIKVSNQEMDALNISGDAFHPE"
     gene            complement(1433..1816)
                     /locus_tag="VGE92_09265"
     CDS             complement(1433..1816)
                     /locus_tag="VGE92_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314056.1"
                     /translation="MGKRRAKHPFEDNPVIDGFLEWMDAPEGQQSIEALDLVFAALEQ
                     AGVDAGQRKIVWADGKRLSIGQSAERIHAGHPGVPRDLIETHVLGWLESCAPESCSER
                     QLEELDRLIEPWLDDYERTSRAGRK"
     gene            1956..>2655
                     /locus_tag="VGE92_09270"
     CDS             1956..>2655
                     /locus_tag="VGE92_09270"
                     /inference="COORDINATES: protein motif:HMM:NF024935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY1314057.1"
                     /translation="MSLWIPWWNAIWLLRPAFSRLRAFLWFATAVAGFAVRTELLGVT
                     SLVRGLKLDARFYNKLVDHFHSPAVQLDRLAALWTQVVLRLFPQPLRVNGRCVVVGDG
                     IKVPKRGRKMPGVKLLHQQSESNTKPEYIMGHSLQAVSLLVQAAASVFAVPLAMRIHE
                     GVVWSNRDRRTLLDKMLALLGIVAIAQPCYFVADAYYAARKIVIGLLKDNNHLVTRVK
                     SNAVAYTPYQQRGPR"
BASE COUNT          520 a          851 c          789 g          495 t
ORIGIN      
        1 gtcctgtgcc atcatacccc tccattcccc gcctggggcg cagcttagac cggggtatct
       61 tacgcctcaa ctatggcgac gcccaaagtc tattgaatcg cggggcgtga tggctccagg
      121 atttttgatg agggagattc cggcgcggtc gagaccctca tagactccgg ggatgataga
      181 cacatcagcg attcgcgagc ggttcgcggc ggtttcccgg gacctgaacg aacggtctcg
      241 ccgtcttctc gcggcggcgg aagccaaaac ggcaggtcat ggcgggatcg ccgcggcgtc
      301 gcgggcgacc ggtatcgcac gcagcacgat tggccggggc ctgaaggacc tggctgctcc
      361 ggacgggttg tccggcgata tccggcggcc cggcggcggc tgtccaacac tggtcgataa
      421 ggatccggct cttctggatg acctgcggcg gctggtggaa cccgcgacca tgggcgatcc
      481 gacgcggccg ctgatgtggg tatcgaaaag tcatgcgaag ctggctgccg ccttgcgtga
      541 catgggccac aagatcgctg acagcagtat cccgaaacta ctgggcctgc tggactaccg
      601 ccggcaggtg aaccggaaga cactggaagg tagtcataat cctgaccgga acgcgcaatt
      661 cgaacatatc aacgctgcgg tgaccgccac tcaggcgatg ggtcaaccgg tgatttcaat
      721 cgataccaag aagaaggaac tgatcggcgc ctacaagaat ggcggcagcg attaccggcc
      781 ggaaggctgt cccgacaagg tcaatgtgca tgatttcatc gacaaagacc ttgggaaggc
      841 gatcccctac ggcgtttacg atatcgccgc caatgccggt tgcgtgagtg tcggtatcag
      901 caacgataca gcgcagttcg cggttaattc gatccgtcac tggctggaca cgatgggccg
      961 cgaacgatat ccggccatgc agcagttgat gatcacggcg gacggcggcg gttcgaacgg
     1021 atcgcgcgtg cgtctgttca agatcgaact tcaaagactg gccgacgaaa ccggcctgac
     1081 ggtctgcgtc tgccattatc cgccggggac gtcgaagtgg aacaaaatcg aacaccggct
     1141 gttctgccac gtcacgcaaa cctggcgcgg aaaacccttg gtcagccgtg aaacagtggt
     1201 cgaattgatt gccgccacca caacccggac cggcctgatc gtacgatgtg cgttggatac
     1261 acgcgattat cccaagggca tcaaggtcag caaccaggag atggacgccc tcaacatcag
     1321 cggcgacgct ttccacccgg aatagaatta tacgatctca cccagaccgc caatctgatg
     1381 cgatagattt tgggcgtcgc ctaaccctag ctcgagagtg ccgagttctc ggctactttc
     1441 tgccggctcg tgacgtgcgc tcatagtcgt cgagccacgg ttcgataagc cggtcgagtt
     1501 cctcaagctg gcgctcggag caggattccg gcgcacagct ttccagccaa cccagcacgt
     1561 gagtttcaat caggtcgcgc ggcacgcccg gatgcccggc gtggatgcgc tccgccgatt
     1621 gcccgatcga tagccgtttc ccgtcggccc agacgatctt gcgctgcccg gcgtcgacgc
     1681 cggcctgttc cagtgcggcg aacaccaggt cgagtgcctc gatcgactgt tgtccctcgg
     1741 gcgcgtccat ccattcgaga aaaccgtcga tgacggggtt gtcctcgaac gggtgtttcg
     1801 cgcgccgctt gcccatcgga tgaggatagc accgccggcc accgctgtcg cgagactcac
     1861 gatagattca acgtcgatca caatagattc aacattgtta cagtgattct cggggtacgc
     1921 gcatcgttca tcgaaaacag caacgaatcg gcccgatgtc actctggatc ccctggtgga
     1981 acgcgatctg gctgctgcgc ccggcgttct cccgtctgcg cgccttcctc tggttcgcca
     2041 ccgccgtcgc aggcttcgcc gtgcgcaccg aattgctcgg cgtcaccagc ctcgtgcgcg
     2101 gccttaagct cgacgcccgc ttctataaca agctggtcga tcactttcac agccccgccg
     2161 tgcagctcga ccggctcgcc gcgctgtgga cccaggtggt gctgcggctg ttcccacagc
     2221 cgctgcgcgt caacggccgc tgcgtcgtgg tcggcgacgg catcaaggtg ccaaagcgcg
     2281 gccgtaagat gccgggggtc aaactcctgc atcagcagtc agagtcaaac accaagcccg
     2341 aatacatcat gggacactcc ctccaggcag tgagcctgct cgtgcaggcc gccgccagcg
     2401 tcttcgccgt gccgctcgcc atgcgcatcc acgagggagt ggtgtggtcc aatcgcgacc
     2461 gccgcacctt gctcgacaag atgctcgccc tcctcggcat cgtcgccatc gcgcaaccct
     2521 gctacttcgt cgccgacgcc tactatgccg cgcgcaagat cgtcatcggc ttgctcaaag
     2581 acaacaatca cctggtcacc cgggtgaagt ccaacgccgt cgcctacacc ccgtaccagc
     2641 agcgcggccc gcgca
//
LOCUS       DASWZT010000339         7498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_65546, whole genome shotgun sequence.
ACCESSION   DASWZT010000339 DASWZT010000000
VERSION     DASWZT010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7498
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65546"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VGE92_09275"
     CDS             <1..250
                     /locus_tag="VGE92_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011532564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEY1314058.1"
                     /translation="ANGFSELNDPEDQAQRFKDQVERKDAGDEEAMYYDADYVRALEY
                     GMPPAAGLGLGVDRLVMLFTNSPSIRDVLLFPHMRPEL"
     gene            247..1044
                     /gene="murI"
                     /locus_tag="VGE92_09280"
     CDS             247..1044
                     /gene="murI"
                     /locus_tag="VGE92_09280"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEY1314059.1"
                     /translation="MIPIGVFDSGVGGLCVLRALRAAMPTQDFIYLGDTARLPYGTKS
                     AATVLRYSLQCAAALVQRRIRCLVVACNTASASALDALRTLYPSLPVVGVIEPGAAAA
                     VAASVSQHIAVIGTEGTIAGGAYQHAIQRLNRGTRVTAQACSLFVAMAEEGWTEGAIA
                     EAVAHRYLDPIFRVDDAPDTLVLGCTHFPALMAALRAVLPSHVNIVDSAATTASAVLR
                     QVGASGTPGTPGEISWLATDGAARFARVGSTFLGEMLRPDAIEIIDL"
     gene            complement(1041..1955)
                     /locus_tag="VGE92_09285"
     CDS             complement(1041..1955)
                     /locus_tag="VGE92_09285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314060.1"
                     /translation="MSVPAQPVSGLLAYLGILRFVGPDSLSFLQGQVSNDTRLLAQGR
                     SLLAAHSSPQGRVLALLRILPHSNGVSAILPRELVVPTLEALRKFVLRAKVKIEDASD
                     ALRVVGVHGAHRLESAGLAAPPSSTASEAHGLGVAAVNGDSGRYWVIGPEETLSQRGL
                     GPGVASAEQIERDWRLADIRAGLPQIYLTTREAFVAQMLNLDLLDGISFTKGCYTGQE
                     IIARTQHRGRIKRRLYRLRLPPGEWQVGQTLRLTDGRSGRVTEAVSVAGGFEALAVLN
                     VEPADGEAEPGAAVAADPLPLPYALQVP"
     gene            2045..2191
                     /locus_tag="VGE92_09290"
     CDS             2045..2191
                     /locus_tag="VGE92_09290"
                     /inference="COORDINATES: protein motif:HMM:NF019511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1674 domain-containing protein"
                     /protein_id="HEY1314061.1"
                     /translation="MIQSESEPKPDGGSPPPAEAVPREVGGREGPEPTRFGDWELRGR
                     CIDF"
     gene            2194..2574
                     /gene="sdhC"
                     /locus_tag="VGE92_09295"
     CDS             2194..2574
                     /gene="sdhC"
                     /locus_tag="VGE92_09295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEY1314062.1"
                     /translation="MPRPISPHWQIYRWQIGNSLSILHRATGVALAFGLFALCYWLVS
                     LAGGQRSYALALKVLASPIGRICLIGWTFAFSYHLLNGVRHLFWDAGYGFGRSQRHAS
                     GWFAVLGAFVLTLGVWALIWHAAP"
     gene            2571..2951
                     /gene="sdhD"
                     /locus_tag="VGE92_09300"
     CDS             2571..2951
                     /gene="sdhD"
                     /locus_tag="VGE92_09300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEY1314063.1"
                     /translation="MSLRSPLGRVLGLGSAKDGTAHWWAQRVSAVALVPLTLWFMISL
                     LALPSLDYATVRIWLSFPLTALLTMLLVAVLTYHSYLGTSVVIEDYVHSAGTKVASLL
                     LLRFLYVLVGAASIFAVLRIALRS"
     gene            2957..4738
                     /gene="sdhA"
                     /locus_tag="VGE92_09305"
     CDS             2957..4738
                     /gene="sdhA"
                     /locus_tag="VGE92_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEY1314064.1"
                     /translation="MSEYKLIEHTYDVVVVGAGGAGLRATFGLAEAGLSTACLTKVFP
                     TRSHTVAAQGGISAALGNMGVDDWRWHMYDTVKGSDWLGDQDAIEFMCKEAPAAVIEL
                     EHYGVPFSRTEDGRIYQRPFGGMTTHFGKGIAQRTCAAADRTGHAMLHTLYQQSLKHE
                     ANFFIEYFALDLIMENGECRGVIALDMSDGSLHRFRAHMVIMATGGYGRAWFSCTSAH
                     TCTGDGGGMALRAGLPLQDMEFVQFHPTGIYGAGCLITEGSRGEGGYLTNASGERFME
                     RYAPNAKDLASRDVVSRAMTVEIREGRGVGALKDHIDLHLEHLGPAVIKERLPGIAET
                     ARIFAGVDVNKEPIPVLPTVHYNMGGIPCNVHGEAITRANGAETVVRGLMAVGEAACV
                     SVHGANRLGSNSLLDLVVFGREAARHCARVLKPDSPHRAFKSDACDNALARLDKYRHA
                     GGSRRTADVRLAMQRVMQSDAAVFRTGESLDDGKRRLAEVFASFSDVQVTDRSLVWNT
                     DLIETLELDNLLGQAVATMHSAANRQESRGAQAREDFPNRDDQNWLKHSLCWVDAHGA
                     TRFDYRPVHLNTLTDEVESIPPKARTY"
     gene            4766..5551
                     /locus_tag="VGE92_09310"
     CDS             4766..5551
                     /locus_tag="VGE92_09310"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005096284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEY1314065.1"
                     /translation="MAKFSLPANSKVRRDGRVFKAPAGAKNVRVFKIYRFDPDSAENP
                     RLDSYEVDMDDCGPMVLDALIKIKNEIDSTLSFRRSCREGICGSCAMNIDGKNGLACV
                     SACIDVKNDIKIYPLPNMPVIKDLIPDLTNFYAQYASIKPWLQTRTPAPPDGERLQSK
                     EDQERVDRPSACILCACCSTSCPSYWWNSDRYLGPAALLAAYRWIVDSRDEATGERLD
                     YLEDPFRLYRCHTIMNCTEACPKDLNPAKAIAEIKQMMIHRRL"
     gene            5551..5793
                     /locus_tag="VGE92_09315"
     CDS             5551..5793
                     /locus_tag="VGE92_09315"
                     /inference="COORDINATES: protein motif:HMM:NF015870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEY1314066.1"
                     /translation="MAEELGKLRWRCRRGIKELDVLLTRYVDERFCSASELEQEAFRQ
                     LLESQDPVLYGYCFGSEPPPAQFAALIERITAFGGR"
     gene            5939..6541
                     /gene="rpoE"
                     /locus_tag="VGE92_09320"
     CDS             5939..6541
                     /gene="rpoE"
                     /locus_tag="VGE92_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003172477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HEY1314067.1"
                     /translation="MSVEESDLKLVERVQRGERAAFDLLVLRYQHKVLKLIMRYVHDA
                     TEAEDIAQEAFVKAFRALQSFRGDSAFYTWLYRIAINTAKNALVATKRRPMDYDLDLQ
                     DPEQYDLHARLAESETPEGLLLTDEIRDTVNRAIENLPEDLRTAIVLRELEGLSYEDI
                     AQTMECPVGTVRSRIFRAREAIDKKLRPIFDGGLGRPEDT"
     gene            6538..7155
                     /locus_tag="VGE92_09325"
     CDS             6538..7155
                     /locus_tag="VGE92_09325"
                     /inference="COORDINATES: protein motif:HMM:NF015810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HEY1314068.1"
                     /translation="MSEQIREQVSAFLDGELPNSETELLLKRLTRDEDLRESFGRYAL
                     IGEAVRGSSVSLRTKGFAGRVNLAIDGESVPADAHARKVREPHWWRPFAGAAVAAGVA
                     AVAVVAFQQRAVAPTLRPVTAQNAVISPGGAVAVATSRENLSYTVPPTSAAIPAVMPR
                     TRLTNYVFAHSRYSSGLDQRGVLADMLIEAEEQQPTVDAAAHAAP"
     gene            7152..>7498
                     /locus_tag="VGE92_09330"
     CDS             7152..>7498
                     /locus_tag="VGE92_09330"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HEY1314069.1"
                     /translation="MTGVARNRIHWLMPCAVVGALFWATPLLTHAADDPRDWLQKMNQ
                     ALATRNYDGTFFHLSEGRIETMRIVHRVRAGRVTERLQSLDGSGREFVRDNDELTCYL
                     PDQHTVLVQPRPE"
BASE COUNT         1353 a         2224 c         2418 g         1503 t
ORIGIN      
        1 tgcgaatggt ttttcggaac tgaacgaccc tgaggatcaa gcgcagcgct tcaaggacca
       61 ggtcgaacgc aaagatgccg gcgatgagga agccatgtac tacgatgccg actacgtgcg
      121 cgccctggaa tacggtatgc cgccggcggc cgggctgggg ttgggtgtcg accgattggt
      181 catgttgttc acgaactcgc cgtcgattcg cgatgtgctg ctgttccccc acatgcggcc
      241 ggaactttga tcccgatcgg ggtgttcgat tccggtgtgg gcgggctgtg cgtgttgcgg
      301 gcgctgcgtg cggcgatgcc gacgcaggat tttatttatc ttggcgatac cgcacgtcta
      361 ccctacggta cgaaaagtgc ggccacggtc ctgcgttatt ctctgcagtg cgcggcggcg
      421 ctggtgcagc gacggatccg ttgtctcgtg gtcgcgtgca acacggcgtc ggcgtcggca
      481 ctcgatgcgt tgcgtacatt gtacccatcg ctgccggtgg tcggagtgat cgagcccggc
      541 gcagcggcgg cggtggccgc ttcggtttcg caacacatcg ccgtcatcgg aaccgaaggg
      601 actatcgccg gcggggctta ccaacacgcc attcagcgtt tgaatcgcgg cacgcgagtg
      661 accgcccagg cgtgctcttt atttgtggca atggccgaag agggatggac cgaaggcgcg
      721 attgccgagg cagtcgcaca tcgctacctc gatccgatct ttcgagtcga cgacgcgccc
      781 gataccttgg tgctcggatg tacgcacttc ccggcgctga tggcggcgct gcgcgcggtg
      841 ctgccgtcgc atgtaaacat tgtcgactcg gcggcgacca ctgcgagcgc ggtgctgcgg
      901 caagtcggcg catccgggac gccgggcaca cccggcgaga tctcgtggtt ggctacggac
      961 ggcgcggcgc gcttcgcaag ggtgggcagc acgttcttgg gcgagatgct gcgccccgat
     1021 gccatcgaga tcatcgatct ttaagggact tgcagcgcgt aaggtagggg tagtgggtcg
     1081 gcggcgactg ccgcacccgg ctcggcttcg ccgtctgccg gttcgacgtt gagtactgcg
     1141 agcgcctcga aaccgccggc aacgctcacc gcctcggtca cgcgtccgga gcggccgtca
     1201 gtcaagcgca gcgtttgacc gacctgccac tcgccgggcg gcagccgcaa tcgatacagg
     1261 cgccgcttga tgcggccccg gtgctgagtg cgcgcgatga tctcttgtcc ggtgtaacag
     1321 cccttcgtaa agctaatccc gtccaacaag tcgaggttca gcatctgcgc gacgaaggct
     1381 tcgcgggtcg tcagatatat ctgcggcagt ccggcgcgaa tatccgcgag acgccagtcg
     1441 cgctcgattt gctcggcgga cgccacgccg ggcccaagtc cgcgctgcga caatgtctcc
     1501 tcgggtccga tgacccagta acggcccgag tcgccgttca cggcggcaac gcccaggccg
     1561 tgcgcctctg aagccgtgct cgatggcggg gcggcgaggc cggctgactc aagacggtgc
     1621 gcgccatgca cacccacgac gcgcaaggcg tcactggcgt cttcgatctt cactttggcg
     1681 cgcagcacga acttgcgcag cgcctccagc gttggcacca cgagttcacg gggcaggatt
     1741 gcgctgaccc cgttggagtg gggcaggatg cgcaggagcg cgagtacgcg gccctgcggg
     1801 cttgaatgag cagccagcag gctgcgtcct tgcgccagca gacgcgtgtc gttcgacacc
     1861 tggccttgta gaaaactcaa ggaatcgggc ccgacgaatc gcagaatgcc taaataggcc
     1921 agaaggcccg atacgggctg cgctggaact gacatcgccg tatgaagtgg gtactcgagg
     1981 agcgaagtca accctaattc agccaatcaa ggattgatct tttctggcaa actttgcagc
     2041 cgtaatgatc cagtctgaat ccgaacccaa acctgacggt ggctccccgc cgcccgccga
     2101 agctgtgccg cgcgaggttg gcggccgcga agggccggag ccgacgcgct tcggcgattg
     2161 ggagctgcgc ggccgctgca tcgacttcta agaatgccgc gtccgatttc accccattgg
     2221 caaatctatc gatggcagat cggcaatagc ttgtcgatct tgcatcgcgc gaccggcgtt
     2281 gccctggcct tcggtctgtt cgccctttgt tattggctgg tgtcgctggc gggcgggcag
     2341 cgttcctatg cgctggcgct caaagtgttg gcaagtccca tcggacgtat atgtctcatc
     2401 ggttggacct ttgctttttc gtaccatctg ctgaacggcg tgcgccattt gttttgggat
     2461 gcaggttatg ggttcgggcg cagccaacgg cacgcgagcg gctggttcgc agtgctcggc
     2521 gcgtttgttc tgacgctggg cgtctgggcg ctgatctggc acgccgcgcc atgagtttgc
     2581 gcagtccttt gggacgtgtc ctgggtttgg gatcggccaa agacggcacc gctcattggt
     2641 gggcacagcg ggtgagcgcc gtggcgctgg tccccctcac cctttggttc atgatttctc
     2701 tgctcgcact gccgtcgctc gattacgcca cggtgcgaat ctggctgtcc tttccgttga
     2761 cggcattgct gaccatgctg ctggtcgcgg tgctcacgta ccactcctat ctgggaacca
     2821 gcgtggtgat cgaggattat gtgcattcgg ccggtaccaa ggtcgcctcg ctgctgctgc
     2881 tgcgatttct gtacgtgctg gtcggggccg cgagcatttt cgccgttttg cgcattgcgt
     2941 tgagatccta gagaccatga gcgaatacaa actgatcgag cacacttacg atgtcgtcgt
     3001 cgtcggtgcg ggaggcgctg gcttgcgcgc caccttcggt cttgccgagg ccggtctgtc
     3061 cacggcctgc cttaccaagg tattccccac ccgcagccac acggtggcgg cgcagggcgg
     3121 catctcggcg gcgctcggca acatgggcgt cgacgattgg cgctggcata tgtacgacac
     3181 cgtcaagggg tcggattggc tcggcgatca agacgcgatc gagttcatgt gcaaggaggc
     3241 gccggcggcg gtcatcgagc tcgagcacta tggagtgccg ttttcacgca ccgaagacgg
     3301 ccgcatctac cagcggccgt ttggcggcat gaccacgcac ttcggcaaag gaatcgcaca
     3361 acggacctgc gccgcggccg accgtaccgg ccatgccatg ctgcacactc tgtatcagca
     3421 gtcgctcaag cacgaagcga actttttcat cgaatacttc gccctcgatc tcattatgga
     3481 aaatggcgag tgccgcggag tcattgctct cgacatgtcc gacggttcgc tgcatcgatt
     3541 ccgtgcccac atggtcatca tggcgaccgg cggttacggg cgagcatggt tttcgtgcac
     3601 ctccgcccac acctgcaccg gcgacggcgg cggcatggcg ttgcgtgcgg gcctgccctt
     3661 gcaggacatg gagttcgtgc agttccatcc gaccggcatt tacggcgccg gttgcctgat
     3721 caccgaagga tcgcgcggcg agggcggcta tctgacgaat gcctcgggcg agcgtttcat
     3781 ggagcgctac gcgccgaatg ccaaggactt ggcgtcgcgc gacgtggtga gccgggcgat
     3841 gaccgtggag attcgcgagg gccgcggagt gggagcgctc aaggaccata tcgacctgca
     3901 tttggagcat cttggcccgg cggtcatcaa ggagcggctg cccggaattg ccgaaactgc
     3961 gcggatattc gcgggtgtgg acgtcaataa ggagccgata cccgtattgc ccaccgtgca
     4021 ctacaacatg ggcggcatac cctgcaacgt gcacggcgag gcgatcaccc gggcgaacgg
     4081 cgccgagacg gtggtgcgcg gtttgatggc cgtgggcgag gcggcctgcg tatcggtgca
     4141 cggtgcgaac cgcttgggat cgaactcgtt gctcgatctc gtggtattcg gccgcgaagc
     4201 ggcccgacat tgtgcgcgcg tgttgaaacc ggatagcccg catcgcgcct tcaagagcga
     4261 cgcctgcgac aatgccctgg ctcgcttgga caaatatcgg catgccggcg gctcgcgacg
     4321 taccgccgat gtgcgcttgg caatgcagcg cgtcatgcag agcgatgccg cggtatttcg
     4381 caccggcgag tcgttggatg acggcaaacg caggcttgcg gaagttttcg cgtctttttc
     4441 ggacgttcaa gtcacggacc gctcgctggt gtggaacacc gatttgatcg agaccctcga
     4501 gctcgacaat ttgctcgggc aggcagtcgc cacgatgcat tcggcggcga accgtcagga
     4561 gagccgcggg gcgcaggccc gcgaggattt cccgaatcgc gacgaccaaa attggctgaa
     4621 acacagcctc tgttgggtgg acgcccacgg agccacacgc ttcgattatc ggcccgtgca
     4681 cttgaacacc ctgaccgacg aggtcgaatc gataccgccc aaggcgcgga cctactgaag
     4741 cgaagcgtat gcccaggagc atttgatggc taaattctcg cttcccgcca attcaaaggt
     4801 gcgcagggac ggccgtgtct tcaaagcgcc ggcgggcgcc aagaatgtgc gggtattcaa
     4861 aatttaccgt ttcgacccgg actctgcaga gaatccgcgg ctcgacagct acgaagtcga
     4921 catggacgat tgcggtccga tggtgctcga cgcgctcatc aagatcaaga acgagatcga
     4981 ctctaccttg agctttcggc gctcgtgccg cgaggggatc tgcggctcgt gcgcgatgaa
     5041 tatcgacggc aagaacggcc tcgcctgcgt cagtgcctgc attgacgtga agaacgacat
     5101 caagatctat ccattgccga atatgccggt aatcaaggat ttgatccccg atctgaccaa
     5161 cttctatgcc caatatgcct cgatcaagcc atggcttcag acccgtactc ctgcaccgcc
     5221 cgacggcgaa cgactgcagt ccaaggagga tcaggagaga gtggatcggc cctcggcgtg
     5281 tattttgtgc gcctgttgct caaccagctg cccgagctac tggtggaata gcgatcgcta
     5341 tctggggcct gcggcgctct tggccgcgta ccgctggatc gtcgatagcc gcgatgaagc
     5401 cacgggcgag cgcctcgact atctcgagga tccgttcagg ctataccgct gtcataccat
     5461 catgaattgt acggaggcct gccccaagga tctgaatccc gccaaggcca tcgccgaaat
     5521 caagcaaatg atgattcatc gccgtctttg atggccgagg aactcgggaa gctgcgttgg
     5581 cgctgccggc gcggcatcaa ggaactcgac gtattgctga cgcgctatgt ggatgagcgg
     5641 ttctgcagcg catcggagct cgagcaggag gcttttagac agttgctgga gagccaagat
     5701 ccggttctat atggttattg cttcggatcc gagcctccgc cagcgcaatt cgcagctctg
     5761 atcgagcgaa tcaccgcgtt cggcgggcgc tagctcggag cccaccgctg ttagaatccg
     5821 cccctgtgcg ccagatcacg tttaaaagcg gttcagtccg gaaattgtcg aacttttccc
     5881 caaccggtcg gtctatcgcg acaggctggg ttgtgagctc gccggtttgg gatcacgcat
     5941 gagtgttgaa gagagtgatc tcaaactggt cgagagagtc cagcgtggcg agcgcgcggc
     6001 gttcgatttg ttggtcctgc gataccagca caaggtgctg aagctgatca tgcgttacgt
     6061 gcacgatgca accgaggctg aagacatcgc tcaggaggct ttcgtcaaag cattccgtgc
     6121 attgcagtcg tttcgcggcg atagtgcgtt ttatacctgg ctgtatagaa ttgcgatcaa
     6181 taccgccaag aacgccctgg ttgccaccaa acgccgcccg atggattacg atctcgatct
     6241 tcaggatccc gaacaatacg atttgcacgc gcggctggcc gagtccgaga ctccggaagg
     6301 tctgctgctc acggatgaga ttcgcgacac cgtgaatcgc gccatcgaga atctaccgga
     6361 ggatctgcgc acggcgatcg tgctgcgcga gttggaaggt ttgagttacg aggatatcgc
     6421 gcaaacgatg gaatgcccgg tgggaaccgt gcgctcccga atatttcgcg cgcgcgaggc
     6481 gattgacaaa aagttgcgac ccattttcga tggcggcctt ggtcggcctg aggacacatg
     6541 agcgaacaaa ttcgtgaaca ggtctcggcg tttctcgacg gcgaattgcc caactcggaa
     6601 accgaattgc tgctcaagcg gctgactcgc gatgaggatt tgcgcgagag tttcggccgc
     6661 tatgccttga tcggcgaggc ggtgcgcggc tcgagcgtca gcttgcggac caaggggttt
     6721 gccggtcgcg tgaatttggc gatcgacggc gagagcgtcc cggccgatgc gcatgcgcgc
     6781 aaggttcgcg aaccgcattg gtggcgtccg ttcgccggcg ccgcggttgc ggctggcgtg
     6841 gcggcggtgg ccgtcgtggc gttccaacag cgggccgttg cgccgacgct gcggcccgtg
     6901 acggcgcaga acgcggtcat ttcaccaggc ggcgcggtcg cagttgccac ctcccgggag
     6961 aatctctcct ataccgtgcc gccgacttct gcggcgatac ccgctgtcat gccacggacg
     7021 cgcttgacga attacgtgtt tgcgcatagc cgctattcct ccggtctgga tcagcgcgga
     7081 gtgttggccg atatgctcat cgaagccgag gaacagcagc cgacggtcga cgcggcggcg
     7141 cacgcggctc catgacggga gttgcccgca atcgcatcca ttggctgatg ccctgcgccg
     7201 tggtcggcgc tttgttctgg gccacgccgc tgcttaccca tgctgccgac gacccgcggg
     7261 attggctgca aaaaatgaac caagccttgg cgacgcgcaa ctacgacggc acgtttttcc
     7321 atttgagcga gggccggatc gagaccatgc gcatcgtgca cagggtgcgc gccgggcggg
     7381 tgaccgagcg gctgcagtcc ttggatggtt ccggccggga attcgtgcgt gacaatgacg
     7441 aattgacttg ttatttgccg gatcagcaca cggtgctggt acagccacgg cccgagca
//
LOCUS       DASWZT010000340         6882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_12476, whole genome shotgun sequence.
ACCESSION   DASWZT010000340 DASWZT010000000
VERSION     DASWZT010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6882
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12476"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VGE92_09335"
     CDS             <1..192
                     /locus_tag="VGE92_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314070.1"
                     /translation="GLSSANLQGLPSYPSRSPRKELDFILHSAQIEVNRFEIPDVQFS
                     DHLPLICDFTIHQRVTQAA"
     gene            192..2222
                     /locus_tag="VGE92_09340"
     CDS             192..2222
                     /locus_tag="VGE92_09340"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY1314071.1"
                     /translation="MAQSRNWSLEFDSDRVAWLVCDMPGTSTNVLSAGVLRELALQLG
                     TVAANKAAGLVVRSAKSNGFIAGADIKEFLKIESPEQGYELVRAGQAVLESLSSLPCP
                     SVAALNGFALGGGLELALACTYRVAADDATLSLGLPEVLLGLHPGFGGTVRTVQLIGV
                     RAALDLMLKGKPCKGSRALALGLIDELVPPAELLARAKSRLLRAPPRRRAPFIDKLLN
                     VGAVRPFIARQAIATLRASVRRDQYPAPYSIVDLWQRYGSGGMASYDAEARSIALLMC
                     GSTSRNLVRVFLLQDRLKGLGGQSSAEFRHVHVIGAGVMGGDIAAWAAFRGMTVTLQD
                     RSEELIRPSLARAKSFFDKRLKDPAAAAAALARLRMDVSGDGVGDCDVVIEAIFENVD
                     AKRALYAALEPKLKPSAILATNTSSIRIETLCEKLADPSRLVGIHFFNPVAQLQLVEI
                     VQGAGTGAQVVQNALKFTRKLDKLPLPCRSAPGFVVNRILTPYINEALFALESGIPAA
                     VIDRAGKGFGMPMGPLELTDVVGLDVSLHVGRVLAEAFGRPVPDILVKLVEQKKLGRK
                     SGEGFYVWRDGKPVKPEPPPQIAAPADLEDRLILPMLNEAVACLREAVVQDADLLDAG
                     VIFATGFAPFRGGPLHYAKQRGIANITGRLAELATQYGERFRPDPGWQEFPG"
     gene            2328..3392
                     /locus_tag="VGE92_09345"
     CDS             2328..3392
                     /locus_tag="VGE92_09345"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY1314072.1"
                     /translation="MDERPLDLSVLKTFSPLDGLKAENLHALARKTQIRELGAGRVLF
                     KEGDSDKRTFYLVSGSVELRADDRTVGTIRSGSPEARAALAPGLPRKFTARASVDIEY
                     IVIDSDLLDVLLTWDQTGQYEVAELRGDGVDVTGDWMTTLLQTKAFHRIPPANIQAIF
                     MRMQRINYRASDVVIKQGTEGDYFYVVVAGKCIVTRETPLNKEGIKLAELGPGDSFGE
                     EALIAEAKRNATVTMSTDGTLMRLGKSDFQTLLNEPLLQWVDYPKALEIVAGGGKWLD
                     VRLPSEFQNFRIDGAVNIPLYFIRLKLNALDKNTQYVVCCDTGRRSSAAAYILSERGF
                     EAYVLKGGLAATEAARSHPG"
     gene            3399..3641
                     /locus_tag="VGE92_09350"
     CDS             3399..3641
                     /locus_tag="VGE92_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314073.1"
                     /translation="MSRVFRGEKSRGFPEPGELQEFKARSMIEEYRGLAIVFAVIAVA
                     LAVYFIKWIVLAPTPAPPPVQSVYIDVVPQKPAPSR"
     gene            complement(3647..4018)
                     /locus_tag="VGE92_09355"
     CDS             complement(3647..4018)
                     /locus_tag="VGE92_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003179413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY1314074.1"
                     /translation="MGRNDSKLRSPSNKPGLLTLTIKDKSALYLAYMPFVKNGGLFIP
                     TNSNYRLGDEVFMLLNLMGEEEKLPVAGRVIWMTPKGAQGKRTAGIGVQFSDQDRGNT
                     QKKIENYLAGALGGDKPTHTM"
     gene            complement(4011..5021)
                     /locus_tag="VGE92_09360"
     CDS             complement(4011..5021)
                     /locus_tag="VGE92_09360"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314075.1"
                     /translation="MDFLSADSLPWLADAQRRMRAALAARRLPHALLLLSAPGLGAEY
                     LANWLAALALCESSQPRPCGACASCQLLRADTHPDAYVVRPEENAQQIKIDQVRGLID
                     SLTLKSYRGGYKVGVVEAAEALNASGANALLKTLEEPTADTLLILIARPTHRLPATIA
                     SRCMRLKLAAPPTPAALAWLADRSPADPASAHSWDAALALAGGAPLLALELDGAGLAA
                     LDSDMQESLRQLTAGSVDVTLLADRWIKSDLGLRIAWLENWITRRVYTGLGAAPSHQS
                     AEPVRLPAALLKAKIRALFELLDAARELRRLASTSLNQQLALEALLLGGAAAFANLRV
                     NW"
     gene            complement(5009..5650)
                     /gene="tmk"
                     /locus_tag="VGE92_09365"
     CDS             complement(5009..5650)
                     /gene="tmk"
                     /locus_tag="VGE92_09365"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEY1314076.1"
                     /translation="MSEAGRFLTVEGIEGVGKSTQVARLSASLHERGIAHVVTREPGG
                     TPLAERIRELVLKSGDEPLPDAAELLLMFAARAVHLANHVEPNLKAGRWVLCDRFIDA
                     TYAYQGGGRGLSNDHIRQLDVMVSGARQPDLTVLLDAPVQQALERAAKRNAGAAADRF
                     ESERSDFFERVRAAYRARAAAHPARIAVVNASQSVEQVATQILELLKTRSWIS"
     gene            complement(5647..6681)
                     /gene="mltG"
                     /locus_tag="VGE92_09370"
     CDS             complement(5647..6681)
                     /gene="mltG"
                     /locus_tag="VGE92_09370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY1314077.1"
                     /translation="MSRARSVALLLCVLLLGAGAALQVWLGVQSLDTPLRLSAPVRFK
                     VTPGASFARVAAELSARGVVQSPRAWVVYARLRRLASAVKAGEYEIEPGTTPRELLTK
                     MVNGQVLLHSFTIVDGWRLQDLLGALRRNPDIVTTLGDNAPNLMDKLGLPGVDAEGQF
                     LPETYRFVGGTSDLELLRQAHVALEKELDSAWTGRDPNLPLHDSQQLLIMASIVEKES
                     GLPQELGKIAGLYLHRLAIGMRLQADPTVIYGLGDRYDGDIHTLDLRTDGPYNTYTRS
                     GLPPTPIALPGAAAIHATAHPEKTDAIYFVASAKGDGSHVFSATLQEHNAAVASYLAH
                     LRRQAGEGVR"
     gene            complement(6678..>6882)
                     /locus_tag="VGE92_09375"
     CDS             complement(6678..>6882)
                     /locus_tag="VGE92_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314078.1"
                     /translation="LETAGDLELRAVERRLTWQDLRQAEEVFMSNAVVGVRSVGVIER
                     GKLRLRAPSFEAASRLRARLDSL"
BASE COUNT         1260 a         2197 c         2138 g         1287 t
ORIGIN      
        1 ggtttgagca gcgccaacct gcaaggcttg ccgtcctacc cgagccgcag cccacgcaag
       61 gagctcgatt tcatcctgca cagcgcgcaa atcgaggtca atcgctttga aataccggat
      121 gtgcagttct ccgaccatct gccgctgatc tgcgatttca cgatccatca gagagtcacg
      181 caggccgcct gatggctcaa tcccgcaact ggtcgctgga attcgactcg gaccgggtcg
      241 cttggttggt gtgcgacatg ccgggaacct cgaccaacgt gctgtcggcc ggggtgctgc
      301 gggaactggc gctgcaactc ggcaccgttg cggcgaataa ggcggccgga ctcgtggttc
      361 gctcggccaa atcgaacggc ttcatcgcag gcgccgacat caaggaattc ttgaaaatcg
      421 aaagccccga gcagggctac gagctggtgc gcgccgggca ggctgtcctt gaatccttga
      481 gttctctgcc ctgcccgagc gttgctgccc tcaacggctt tgctctcggc gggggcctcg
      541 agctggcgct tgcctgcacc tatcgagtcg cggccgatga cgcgacgctg tccttgggac
      601 ttcccgaggt gctgctcgga ctgcacccgg gctttggcgg caccgtgcgc accgtgcaac
      661 tgatcggcgt gcgtgcggcg ctcgatctca tgcttaaagg caagccgtgc aagggatccc
      721 gtgcgctcgc gctgggcttg atcgacgaac tcgtgccgcc ggcggagctt ctcgcgcgcg
      781 ccaaatcccg gttgctgcgg gcgccgccgc ggcgccgtgc gccgttcatc gacaagctct
      841 tgaacgtagg cgccgtgcgg cctttcattg cccgccaggc cattgcgaca ctgcgcgcca
      901 gcgtacggcg cgatcaatat ccggcgccct attccatcgt cgatctgtgg cagcgttacg
      961 gctccggcgg catggcaagc tacgatgccg aagcgcgatc catcgcccta ctcatgtgcg
     1021 gttcgacctc gcgcaatctg gtgcgggtgt tcctgctgca ggatcggctc aagggcttgg
     1081 gtgggcagtc gagcgccgaa ttccgccatg tacatgtgat cggcgccggc gtgatgggcg
     1141 gcgatatcgc cgcctgggct gcatttcgcg gcatgacggt gaccttgcag gatcgctcgg
     1201 aggagttgat tcgcccatcc ctggctcgcg ccaagagctt cttcgacaag cgactcaagg
     1261 atcccgcggc ggctgccgcc gcgctggcgc gcttgcggat ggacgtgagt ggcgacgggg
     1321 tcggcgattg cgacgtcgtc atcgaggcca tattcgagaa tgtcgatgcc aagcgtgccc
     1381 tgtacgccgc gctcgagccc aaactgaaac caagcgccat cctcgcaacc aatacctcga
     1441 gcatcaggat cgaaacgttg tgcgagaagc tcgccgatcc gagccgtttg gtcggcattc
     1501 atttcttcaa tccggtggcg caattgcaac tggtagagat cgttcaaggc gcgggcacgg
     1561 gtgcgcaggt cgtgcaaaac gcgctcaagt tcacgcgtaa gctcgacaag ctgccgctac
     1621 cctgccgcag cgctccgggt tttgtggtca atcgcatttt gaccccttac atcaacgaag
     1681 cactttttgc gctggaatcc ggcattcctg ccgccgtgat cgaccgcgcc ggcaagggct
     1741 tcggcatgcc catgggtccg ctcgaattga ccgacgtggt cggtctcgac gtgtcgttgc
     1801 acgtcggccg agtgctcgcc gaggccttcg gacgacccgt gcccgacata ttggtcaaat
     1861 tggtggaaca gaagaaactc ggccgcaaga gcggcgaagg attctacgtt tggcgagacg
     1921 gcaagccggt gaagcccgag ccgccgccgc aaattgccgc tccagcggat ctcgaggatc
     1981 gtttgatcct gcccatgctc aacgaagccg tcgcctgcct tcgcgaggcg gtcgttcagg
     2041 atgccgattt actggacgcc ggcgtgattt ttgcgaccgg ctttgcgcct tttcgcggcg
     2101 gccctctgca ctatgccaag cagcgcggca tcgcgaatat caccgggcgg ctggccgaac
     2161 tcgccacgca gtatggcgag cgttttcggc cggatccggg ctggcaggaa ttccctggct
     2221 gagacacccg tcctgtgatc cactatgtgg tcggacctgc cgtgtcatat ggtaggattc
     2281 gcagccaaaa tcatagggtt gaaccatagg cggcggcaaa tagttcgatg gatgaacgac
     2341 ctctcgatct ctcggtactc aagactttct ctccgctcga cgggttaaag gccgaaaacc
     2401 tgcatgcgct cgcccgcaag acccaaatcc gggaacttgg cgcggggcgg gtcctgttca
     2461 aggaaggcga ttcggacaag cgcacttttt acctcgtctc gggcagcgtc gagctgcgcg
     2521 ccgacgatcg aacggtcggc accatacgat ccggcagtcc ggaggcgcgt gccgcgcttg
     2581 cgcccgggct gccgcgaaag tttaccgccc gcgcgtcggt cgatatcgag tacatcgtaa
     2641 tcgacagcga tctgctcgat gtgctgctga cctgggacca aaccggccag tacgaggtcg
     2701 ccgaattgcg tggcgacggg gtcgatgtca ccggcgattg gatgacaacg ctgttacaga
     2761 ccaaggcgtt tcaccgcatt ccgccggcaa acattcaggc catcttcatg cgcatgcaac
     2821 gcatcaacta ccgcgccagc gacgtcgtga tcaagcaggg taccgagggc gattacttct
     2881 atgtcgtggt cgcgggcaag tgcatcgtca cgcgcgagac gccgctcaac aaagaaggga
     2941 tcaagctcgc cgaactgggt ccgggagaca gcttcggcga ggaagccctc atcgccgagg
     3001 cgaaacgcaa tgccaccgtg acgatgtcca ccgacggcac cctcatgagg ttgggcaaga
     3061 gcgattttca gaccttgctc aacgagcctc tattgcagtg ggtggactat ccgaaggcac
     3121 tcgagatcgt cgccggcggc ggtaagtggc tggacgttcg cctgcccagc gaatttcaaa
     3181 acttccgcat cgacggggcg gtcaacattc cgctgtactt cattcggttg aagctcaatg
     3241 ccttggacaa gaatacgcag tacgtggtgt gctgcgacac cggccggcgc agttcggccg
     3301 cggcctatat cttgagcgag cgcggtttcg aggcctacgt gttgaagggc gggctcgccg
     3361 ccaccgaggc cgctcgctcg catccgggtt gatcggcaat gtcgagggta tttcgcggcg
     3421 aaaaatcgcg gggctttccc gagcctggtg aattgcagga attcaaggcc cgctcgatga
     3481 tcgaagaata ccgcggcctc gccattgtgt tcgccgtgat cgccgtggcg ctggcggtgt
     3541 acttcatcaa gtggatcgtc ttggcgccga cgcccgcgcc gcctccggtg caatcggtgt
     3601 acatcgacgt ggtgccgcag aagccggcgc cgtctcggtg agacggctac atcgtgtgcg
     3661 tgggcttatc gccgcccaag gcacccgcta gatagttttc gatcttcttc tgtgtgttgc
     3721 cgcggtcctg atcgctgaac tgcacgccga tgccggccgt gcgcttgccc tgcgcgcctt
     3781 tgggagtcat ccagatcacg cggccggcga ccgggagttt ctcctcttcg cccatcagat
     3841 tgagcaacat gaacacttcg tcgcccaggc gataattgct gttggtcgga atgaacaacc
     3901 cgccattttt tacaaacggc atgtacgcga gatacagcgc gctcttatct ttgatcgtaa
     3961 gcgtcaatag accgggtttg ttacttggac ttcgcaattt gctgtcgttc ctaccaattg
     4021 acccttaagt tcgcaaaagc cgctgcgccg ccgagcagca aggcctccag cgccagttgt
     4081 tggttcaagc tggtcgacgc caaacgccgc aactcccggg cggcatccag cagctcgaat
     4141 agggccctta tcttggcctt aagtaaagcg gcaggcaagc ggaccggttc cgcactttga
     4201 tgcgaagggg ccgcgcccag gcccgtatat acgcgccgtg tgatccagtt ctcaagccac
     4261 gcgatacgca agccaagatc cgatttgatc cagcggtccg ccaacaaggt gacatcgacc
     4321 gaaccggccg tcagctgccg cagggactcc tgcatgtccg aatcgagagc cgccaatccg
     4381 gcgccatcca attccaaggc gagcagcggc gcaccgccgg caagcgcgag cgcggcgtcc
     4441 caggaatgcg cagacgccgg gtccgcgggg gatcgatcag cgagccacgc gagcgcggcc
     4501 ggcgtcggcg gtgccgccag cttgagccgc atgcagcggc tggcgatggt cgccggtaag
     4561 cgatgcgtcg gtctcgcgat caggatcaag agcgtgtccg cggtgggctc ctccagcgtt
     4621 ttcaacagcg cattggcacc gctcgcattg agcgcctccg ccgcttcgac cacgcccacc
     4681 ttgtacccac cgcgatagct cttgagagtc agcgaatcga tgagtccgcg tacttgatcg
     4741 attttgattt gctgcgcgtt ctcctcgggc cgcaccacgt acgcatccgg gtgcgtgtcg
     4801 gcgcgcagca attgacagga cgcgcatgca ccgcagggtc gcggctgcga agactcgcac
     4861 aacgcgagcg cagcgagcca atttgccaga tactccgcgc ccaagccagg agccgacaac
     4921 aacaacagcg catgcggcag ccgccgcgca gcgagcgcgg cgcgcatgcg tcgttgcgca
     4981 tccgcgagcc agggcaagga gtcggcgctc aggaaatcca cgagcgcgtc ttcaggagtt
     5041 cgaggatttg ggtcgcgacc tgctcgacgg attggctggc gttgacgacc gcgatgcgcg
     5101 ccgggtgcgc ggcagcgcgt gcccggtagg ctgcgcgcac gcgctcgaag aaatcgctgc
     5161 gctcgctttc gaatcgatcc gcggcggcgc cggcgttgcg cttcgcagcg cgctcgaggg
     5221 cctgctgcac cggcgcatcc agcagcaccg tgagatcggg ttggcgggca cctgacacca
     5281 tgacatcgag ctgccggatg tggtcgttgc tcaatccgcg gccgcccccc tgataggcgt
     5341 aggtggcatc gatgaagcga tcgcaaagca cccagcggcc ggcttttagg ttcggctcca
     5401 catggttggc gaggtgcact gcgcgcgccg cgaacatcag cagcagttcc gcggcgtccg
     5461 ggagaggttc atcacctgac ttgagcacga gttcgcggat cctttcggcc aagggcgtgc
     5521 cgccgggctc gcgcgtcacc acatgggcga ttccgcgctc gtgaagcgat gccgacaaac
     5581 gcgccacttg cgtcgacttg cccacgccct cgataccctc gaccgtcaag aaccggcctg
     5641 cttcgctcat cggactcctt ccccggcttg ccgtcgcaaa tgcgcgaggt acgaggcaac
     5701 ggcggcgttg tgctcctgca aggtggcgga aaacacatga ctgccatcgc ccttggccga
     5761 ggcgacgaaa taaatcgcgt cggttttttc cggatgcgcg gtggcatgga ttgcggcggc
     5821 gcccggtaac gcaatgggcg tcggcggcag ccccgaccgg gtataggtgt tgtagggtcc
     5881 gtcagtgcgt agatcgagcg tgtggatatc cccatcgtaa cgatcgccca acccgtaaat
     5941 tacggtagga tcggcctgca agcgcatgcc tatcgccaga cgatgcaggt aaagcccggc
     6001 gattttgccc agttcctgcg gcaagccgct ctccttttcg acgatggaag ccatgatcag
     6061 gagctgctgc gagtcgtgca gcggcagatt cggatcacgg cccgtccagg ccgaatccag
     6121 ctccttctcg agtgcaacat gcgcctgccg cagcagctcg agatcgctcg tgccgccgac
     6181 gaaacgatag gtttcgggca gaaattgacc ttccgcatcg acaccgggaa gtcccagctt
     6241 gtccatcaga ttcggcgcat tatcgcccaa ggtcgtcacg atatccggat tacggcgcag
     6301 ggcgcccagc agatcctgca ggcgccagcc atcgacgatg gtaaatgagt gtaacagcac
     6361 ctggccgttg accattttgg tcaacagctc gcgcggtgtc gtcccaggtt cgatctcgta
     6421 ctcgcccgcc ttgacggcgg aggcaaggcg gcgcaggcgc gcgtacacaa cccaggctcg
     6481 cgggctttga accacgccgc gcgcagacaa ttccgccgca acgcgcgcaa agctcgcacc
     6541 gggtgtgact ttgaatcgaa ccggcgcgct caggcgcaag ggtgtatcga ggctctgcac
     6601 gccaagccat acctgcagtg cggcgcccgc acccagcagc agcacgcaca gcaacagcgc
     6661 caccgaacgc gctcggctca cagtgaatcc aagcgcgcgc gcagccgact cgcggcctcg
     6721 aagctcgggg ctcgcagcct aagcttgccg cgctcgatca caccgaccga ccttaccccg
     6781 acgacggcgt tgctcatgaa cacctcctcg gcctgcctca agtcctgcca cgtcaggcgc
     6841 cgttccaccg cgcgcagctc gagatcgcct gctgtctcca ac
//
LOCUS       DASWZT010000341         5918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_60730, whole genome shotgun sequence.
ACCESSION   DASWZT010000341 DASWZT010000000
VERSION     DASWZT010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60730"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VGE92_09380"
     CDS             complement(<1..452)
                     /locus_tag="VGE92_09380"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1314079.1"
                     /translation="MKSGRSTDAELEFKQLAAGYPQLSGPQLNLGLLYLRDSRLPEAE
                     SAFKAALEHNPNNAVAGNELGIVERKLGKFAEAESAYQHAIAAEPNYAPAHLNLGVLY
                     DLYLAQPQKALDEFQRYIEIAGENKQVAGWVIELRKRVGAPPPAPKKEP"
     gene            451..645
                     /locus_tag="VGE92_09385"
     CDS             451..645
                     /locus_tag="VGE92_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314080.1"
                     /translation="MSSNARSYCSVAGAGIGAASAAGAFEAGVLAGGAAGAPGAADLS
                     GADGAAGVWAAGVDARGGAL"
     gene            complement(676..3510)
                     /locus_tag="VGE92_09390"
     CDS             complement(676..3510)
                     /locus_tag="VGE92_09390"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1314081.1"
                     /translation="MSNPCGMLRAAGAALVAAALAALLAAPCSADSKKPATIKDLDSQ
                     QVDVNTDPPKNVDAGKTMDSYKRFLDLNAGDAALRAEALRRLGDLNLESSESERIERE
                     LVTNEGLRATEAIHLYSALLKTYPKYERNDSVLYQLARAYELNAQPDKALASLDQLVT
                     NYPNSHYVDEAQFRRGEILFTDKAYPKAQAAYEAVIKIGATSGFYNQSLYKHGWSLFK
                     QSENERSLESFAGVLDSVLVSKKSPNDLVELETLSRPNRELVEDTFRVMSITFSYLDG
                     PKSVDEFVKHRKPRPYSYLLYARLGDLYMEKERYTDAADSYRAFVSQDRNNEKAPLLE
                     MQAIEAYQKGGFPQLVLQGKKEFVENYSFGTAYWQGRQPNNEPKVVAVLKTNLKDVAQ
                     HYHAEAQRSKNVADYQEAAKWYRTYLTSFPDDPDSAVTNFLLADTLFESKQYLDAAKE
                     YESTAYHYGDHPKAAAAGYAAIVAYGKQEETLNGDAKAAVHGRNIDSSLKFAQSFPAH
                     PESAQVLTRAAADLYAAKDYPRAIAAAEELLARQPPVDASKQRVAWTVIGNSNFDQGI
                     YDQAETAYTHAEILMPASDPERPVIVERLAASIYKQGEQKSKSGDNTAAVDDFLRVAA
                     LAPTSKVRANADFDAAALLIKDKQWDRAVVVLEGFRRNFPQSPLQADVTRNLAVAYGE
                     SNHPGQAAAQFEQIAQTPGEAPDVQREATLQAADLYDKAGNTAKSRAMLEAFVRHFPQ
                     PLNPAMEARNKLSLITAKSGDTAARDYWLKDIIAADRSAGAARTDRSRALAAKATLTL
                     AIPLREEFKSIKLVAPLKKSLAEKRKAMEAALKAYEGAADYQVADVTTAATFESAELY
                     RQLGKDLMSSERPKNLKKDELEQYNVLLEEQAFPFEEKSIKLHEVNAARAKDGTYDEW
                     VQKSFAALAQLNPGRYGKVEVGEQLVESIR"
     gene            complement(3507..5390)
                     /locus_tag="VGE92_09395"
     CDS             complement(3507..5390)
                     /locus_tag="VGE92_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314082.1"
                     /translation="MINRLRPLILGGLLLALPAAALAKNVKPDPDGLPPQDVKDLHYG
                     DVLFYFYQDDYFDSITRLLAARQLERLPHTQGEAELLLGGLYLSLGEHVEAGRIFEAL
                     LNQNTSEAVRNKAWFYLGKVWYQRGYLDESERALKQVSDKINPRISAERYMLLAQLMM
                     REERYDDAIAALSSWHGAADWTAYAQFNLGIALVRKQRLADAIPYLDRVGRIETRSEE
                     LLSLKDKANLALGFALLQAQRAAEAKPILQRIRLEGPYSSKALLGVGWADAGVGDFKR
                     ALVPWLALRKRSLLDSAVQESFLTVPYAYSQLSASGQAADYYSSAIDSFDAEMKRIDD
                     SIEEIRSGHLLERLLDDDKKDTLTWYWQLSTLPNAPESRYLYQLLASNEFQEGLKNYR
                     ELNFMSRNLDTWRDAASAYDDMLDTRQEAYKRIVPKADAVMAATDLDALTRKRVDFES
                     RINEIEAANDVAALGTPEEQQTWQRLKRIEDYLAAHPDDPDLAEMRDKHRLMRGVMYW
                     RLSASFKARLWNERRSVKELEGDLKETQRRAVLVRQARAAMPSNTGAYAGRVAAIRAR
                     IDQLQQRLSDASQQQNRYLQVLAIRELEGQKLRISTYQIQARYELAAIYDRAANDSAK
                     AKP"
     gene            complement(5403..>5918)
                     /locus_tag="VGE92_09400"
     CDS             complement(5403..>5918)
                     /locus_tag="VGE92_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314083.1"
                     /translation="APAPAAVPAAAPPSDAPGAPPAEFKTLDQEVQGLKKDVIDLNKD
                     LFVLQEELLFPANTQVALYVSIDVGAFFALDSVTIKIDNKVVTNYLYTAREADALLKG
                     GVQQVYLGNLKVGKHELVAFFTGKGPIERDYKRGATINFEKGVGAKYLELKITDHVAK
                     HQPEFAIKDWE"
BASE COUNT          978 a         1847 c         1818 g         1275 t
ORIGIN      
        1 ggctccttct tgggcgccgg cggcggcgcg cctacgcgct tgcgtagttc tatcacccag
       61 ccggcgacct gtttgttctc accggcgatc tcgatgtagc gctggaattc atcgagcgct
      121 ttttgcggct gtgcaagata caggtcgtac agcacgccca aattcagatg cgcgggagcg
      181 taattcggtt ccgcggcgat cgcgtgttga taggccgatt cggcctctgc gaatttgccc
      241 agtttgcgtt cgacgattcc gagctcattg cccgccacgg cgttgttcgg gttgtgttcc
      301 aacgccgcct tgaaggccga ctccgcctcc ggcaagcgcg aatcgcgcag gtacagcaac
      361 cccaggttca attgcggtcc ggacaattgc ggatagccgg cagccagctg tttgaactcg
      421 agttccgcgt cggtgctgcg cccggatttc atgagtagca acgcccgctc gtactgttcg
      481 gtggctggag cagggatcgg tgcggcctct gcggcgggcg cattcgaggc gggcgtgttg
      541 gcgggcgggg cggcaggcgc gccgggagcg gcggatttga gcggtgccga cggcgcggcc
      601 ggggtctggg cagccggggt cgacgcccgc ggcggggcgc tctgacaggc agcgagcagc
      661 gcgacggcgc ctacgctaac gaatcgattc gaccagctgc tcaccgactt ccaccttgcc
      721 gtaacggccc ggattgagtt gcgccaacgc ggcaaagctc ttctgcaccc actcatcgta
      781 cgtgccatcc ttcgcccgcg ccgcgttgac ttcatgtaat ttgatcgact tctcctcgaa
      841 cggaaaggct tgctcctcga gcaacacgtt gtactgctcc aattcgtctt tcttcaaatt
      901 cttaggacgc tcggaactca tcaagtcctt gcctaactga cgatacagct ccgcgctttc
      961 gaaagtcgct gcggtcgtca cgtcggccac ctggtagtcg gcggccccct cgtaggcctt
     1021 gagcgcggcc tccatcgcct tgcgcttctc ggccaacgat ttcttcagcg gcgcaaccag
     1081 tttgatgctt ttgaattcct cgcgcagcgg aatcgccagc gtgagggttg cttttgccgc
     1141 caacgcgcgg ctgcgatcgg tgcgcgccgc cccggcgctg cgatcggccg cgatgatgtc
     1201 cttcagccaa taatcgcgcg ctgcggtatc gcccgacttg gccgtgatga ggctgagctt
     1261 gttgcgtgct tccatcgccg gattcagggg ttgcgggaaa tgtctgacga aggcttcgag
     1321 catggcccgc gacttcgccg tgttgccggc cttgtcgtac agatcggctg cctgcagcgt
     1381 ggcttcgcgc tgcacatcgg gcgcttctcc cggcgtctgc gcaatctgct cgaactgcgc
     1441 cgcggcttga ccgggatgat tcgattcgcc gtaggccacc gcgagattac gcgtcacgtc
     1501 ggcctgcaac gggctctgcg ggaaattgcg gcggaaaccc tcgagcacca cgacggcccg
     1561 gtcccattgc ttatccttga tgagcagcgc cgcggcatcg aaatccgcgt tcgcgcgcac
     1621 tttcgacgtc ggcgccaatg ccgccacgcg caggaaatcg tcgactgccg ccgtattgtc
     1681 gcccgacttg ctcttctgtt caccctgttt gtagatcgac gccgccaggc gctcgacgat
     1741 gaccggccgc tccgggtccg acgccggcat caggatctcg gcatgggtgt aggcggtttc
     1801 cgcctggtcg tagatgccct gatcgaaatt cgaattgccg atcaccgtcc aagcgacacg
     1861 ctgcttgctg gcatcgaccg gcggctggcg tgccagcaac tcctcggcgg cggcgatcgc
     1921 gcgcgggtaa tccttggcgg catacagatc cgcggcggca cgcgtcagca cctgagcgct
     1981 ctcgggatgc gcgggaaatg actgcgcgaa tttcagcgag ctgtcgatgt tgcggccgtg
     2041 aaccgccgcc ttcgcgtcgc cgttcagagt ctcttcttgc ttgccgtagg cgacgatcgc
     2101 agcgtagccg gccgccgccg ctttgggatg gtcgccgtaa tgataggcgg tgctttcgta
     2161 ttccttggca gcgtcaagat attgcttgct ctcgaacagc gtgtctgcga gcaggaagtt
     2221 ggtcacggcc gaatccggat cgtccgggaa tgaagtcaga taggtgcggt accacttcgc
     2281 cgcctcctga taatcggcga cgttcttact gcgctgggct tcggcgtggt agtgctgcgc
     2341 tacgtccttg agattggtct tgagcaccgc aacgaccttc ggctcgttgt tcggctggcg
     2401 cccctgccaa tacgccgtac cgaaactgta gttctcgacg aattccttct tgccctgcag
     2461 cacgagttgc gggaagccgc ccttctgata ggcctcgatc gcctgcatct ccagcaaggg
     2521 cgccttttcg ttgttgcggt cctgtgaaac gaacgcacgg tagctgtcgg cggcgtccgt
     2581 gtagcgctcc ttttccatgt acagatctcc gaggcgcgca tacaggagat acgaataggg
     2641 ccgcggcttg cggtgcttca cgaactcatc gacggatttc ggaccgtcaa gatacgaaaa
     2701 cgtgatgctc atgacgcgaa acgtgtcctc caccaattcg cgattcgggc ggctcaaggt
     2761 ctcgagctcg accagatcgt tcggactctt cttcgacacc aacaccgaat cgagcacccc
     2821 ggcgaaggac tcgagactgc gctcgttttc gctctgtttg aacagcgacc agccgtgctt
     2881 gtagaggctc tgattgtaga atccggacgt ggcgccgatc ttgatgacgg cttcgtacgc
     2941 cgcctgcgcc ttgggatagg ctttgtcggt gaacaggatt tcgccgcggc ggaattgcgc
     3001 ttcatcgacg taatggctgt tgggataatt cgtcaccagt tgatccaagg acgccaacgc
     3061 cttgtcgggc tgcgcgttca attcgtaggc gcgtgccagc tggtacagca cggaatcgtt
     3121 gcgctcatac ttcggatagg tcttcagcaa tgccgagtac aggtggatgg cttcggtcgc
     3181 gcgcaggcct tcattggtca cgagttcgcg ctcgatgcgc tcggactccg agctttcgag
     3241 gttcaaatcg ccgagccggc gcaaggcctc ggcgcgcaat gcggcatcgc ccgcattgag
     3301 gtcgaggaag cgcttgtagc tgtccatggt cttgccggca tcgacgttct tcggtggatc
     3361 ggtgttgacg tcgacctgct gcgagtcgag atccttgatg gtcgccggtt tcttgctgtc
     3421 ggcgctgcaa ggcgccgcca gcagcgccgc cagcgccgcc gccacaagcg ccgcgccggc
     3481 agcgcgcagc atgccgcatg ggttgctcat ggtttggcct tcgcactgtc gttcgcagcg
     3541 cggtcgtaaa ttgcggccaa ctcataccgc gcctgaatct ggtaagtcga gatgcgcagc
     3601 ttctgccctt ccagctcgcg aatggcaagg acctgcaggt agcggttctg ctgctgcgac
     3661 gcatcgctca agcgctgttg caattggtcg atgcgggcgc ggatggcggc gacgcggccg
     3721 gcgtacgcgc cggtgttgct cggcatcgcc gcccgcgcct gcctcaccag caccgcgcgc
     3781 cgttgcgtct ccttcagatc gccttcgagt tccttgaccg agcggcgctc gttccagagc
     3841 ctggccttga agctcgccga cagccgccag tacatgacgc ctctcatcag gcgatgctta
     3901 tcgcgcatct cggccagatc cgggtcgtcc ggatgggccg ccagatagtc ctcgatgcgt
     3961 ttcagtcgct gccaggtctg ctgctcttcc ggcgtgccga gagccgctac atcgttggcg
     4021 gcctcgattt cgttgatgcg cgattcgaaa tcgacgcgct tgcgggtgag cgcgtcgaga
     4081 tcggtggcgg ccatgacggc atcggccttc ggcacgatcc gcttgtacgc ctcttggcgg
     4141 gtgtcgagca tgtcatcgta ggcggacgca gcgtcgcgcc acgtgtcgag gttgcggctc
     4201 atgaaattca gttcgcgata attcttcagg ccttcctgga attcgttgct tgccaacagt
     4261 tggtacagat agcgcgattc gggcgcattc ggcagcgtac tcagttgcca gtaccaggtc
     4321 agcgtatcct tcttgtcgtc gtccaacagt cgctcgagca ggtgcccgct gcggatttcc
     4381 tcgatcgagt cgtcgatgcg tttcatctcc gcatcgaagg agtcgatggc ggagctgtag
     4441 tagtccgccg cctgtccgct cgccgacagc tggctgtaag cgtaggggac cgtgagaaac
     4501 gattcctgca cggcggaatc gagcaggctg cgtttgcgca gcgcgagcca cggcaccagc
     4561 gcgcgtttga aatcaccgac gccggcgtcg gcccagccga cgccgagcag cgccttgctc
     4621 gaataggggc cttcgaggcg tatgcgctgc aatatgggtt tggcttccgc cgcgcgctgt
     4681 gcctgcagca gcgcgaatcc gagcgcgaga ttcgccttgt ccttcaacga cagcaattcc
     4741 tcgctgcggg tctcgatgcg tccgacgcga tccaaatacg gtatggcatc ggcgagccgc
     4801 tgcttgcgca ccagtgcgat gccgagattg aactgcgcgt aagcggtcca gtcggcagcc
     4861 ccgtgccagc tgctcaatgc ggcgatggcg tcgtcgtagc gctcctcgcg catcatcaat
     4921 tgcgcgagca gcatgtagcg ctcggcgctg atccgcggat ttatcttgtc ggagacctgc
     4981 ttcaaagcgc gttccgattc atcgagatag ccgcgctgat accacacctt tcccaaataa
     5041 aaccacgcct tgttgcgtac cgcttccgag gtattttggt tcagcagcgc ttcgaatatt
     5101 ctgcccgcct cgacgtgctc gccgagcgac aagtacaagc cgccgagcag cagttccgcc
     5161 tcgccctggg tgtgcggcag ccgctcgagc tggcgcgcgg ccagcaagcg tgtaatcgaa
     5221 tcgaaataat catcctggta gaaatagaac agcacgtcgc cgtagtgcag atctttgaca
     5281 tcctgcggtg gcaggccgtc gggatccggc ttgacgttct tggcgagcgc cgccgccggc
     5341 aatgccagca gcaatccgcc gaggatgagc ggacgcagtc tgttaatcac acgttgcacg
     5401 catcattccc agtctttgat cgcaaactcg ggctgatgct tggccacatg gtccgtgatc
     5461 ttgagttcca aatacttggc gccgacacct ttttcgaaat tgatggtcgc gccgcgcttg
     5521 taatcgcgct cgatcggtcc cttgccggtg aaaaacgcca ccagctcgtg tttgccgacc
     5581 ttgagatttc ccaaatacac ctgctgcacg ccgcccttga gcagcgcgtc cgcttcgcgc
     5641 gcggtgtaca gataattcgt cacgactttg ttgtcgatct tgatcgtcac cgaatccagg
     5701 gcgaaaaagg cgcccacgtc aatcgacacg tacagtgcaa cctgcgtgtt ggccgggaac
     5761 agcagctcct cttgcaacac gaacaagtcc ttgttcagat cgatcacgtc cttcttcagg
     5821 ccctgcactt cttgatccaa ggtcttgaac tcggcgggcg gagcgccggg cgcgtcgctc
     5881 ggcggcgcag ctgcagggac cgcggcaggt gccggcgc
//
LOCUS       DASWZT010000342         2148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_94774, whole genome shotgun sequence.
ACCESSION   DASWZT010000342 DASWZT010000000
VERSION     DASWZT010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94774"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..1370)
                     /locus_tag="VGE92_09405"
     CDS             complement(174..1370)
                     /locus_tag="VGE92_09405"
                     /inference="COORDINATES: protein motif:HMM:NF016915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314084.1"
                     /translation="MSDLRGASRLAVNAVTGITDLVKALHSAIAKKYTRFAGPIVGEA
                     INGVTDAVYKSIGDVTRGIGAGLDRALEAIGPVLHRLDSSPTREALVAALNGVLGDYL
                     AKSENSLAISMHLRRRGQPLELNREALAASIGEPSTKVVVLVHGLCMNDLRWTRKGHD
                     HGASLERDLSFTAVYLHYNSGLHVSTNGREFANMLDALAAAWPVPLAEVCVIAHSMGG
                     LVARSAHHYASKAGYGWPRKLRAMVFLGTPHDGVPLARVGQWLERLWDKTPYTAPFAR
                     LGKLRSSGIADLRHGYLRDEDWQGRDRLAEECAPRAPLALPEGVECFAIAATLAHARA
                     PLRERLIGDGLVPVPSALGQCREPGFTLAFPASHQRIVYGVSHLDLLSRAVVYSQIRQ
                     WLGWTP"
     gene            complement(1363..>2148)
                     /locus_tag="VGE92_09410"
     CDS             complement(1363..>2148)
                     /locus_tag="VGE92_09410"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEY1314085.1"
                     /translation="GPADDLRALKIPVLADIPEVGGNLQDHLDFCTLNKCTRPVTYDF
                     TQWQEIVVALRYLMTRSGPGVSNVAEAGAFVRTSLAPDARPDVQLHFVPAQLDDHGRN
                     RLDGHGFTIHACVLRPRSRGRLTLRSTLPQNPPRLHAAYLSDARDMDVLLEGIRVSRE
                     IISSRPFAPFRGSEIFPGETVTTRGDLEAMVRRKAETIYHPAGTCRMGSDLKSVVDPQ
                     LRVRGVSGVRVVDASVMPRLIGGNTNAPTMMIAEKAADCVLGE"
BASE COUNT          408 a          682 c          702 g          356 t
ORIGIN      
        1 tttcaaactg aacaatagcg cgcagcgcag gtgcgctaca ttcgcagtaa tacgtaagcc
       61 gctacgtaag agccttttgg gtttacgtgt gcaaacgact cgcccgtggt cgatcaagac
      121 accgcgtcgc cgcaacagca agaggcaaag accaaaagcg ccgcggcagg acctcaggga
      181 gtccaaccga gccattggcg aatctgtgag tacacaacgg cacggctcag caaatccaag
      241 tggctcaccc cgtacacgat ccgctggtgc gatgccggga acgcgagggt gaaccctggt
      301 tcgcggcatt gccccaatgc gctgggcacc ggaaccagac cgtcgccgat caggcgctcg
      361 cggagcggcg cgcgcgcgtg cgccaaagtg gcggctatcg caaagcactc tacgccttcg
      421 ggcaatgcca gcggcgcgcg cggggcgcat tcctctgcga gccggtcgcg cccctgccag
      481 tcttcatcgc gcagataccc atggcgtaag tcggcgatgc ccgagctgcg caatttaccc
      541 agccgcgcga aaggcgcagt gtagggtgtc ttgtcccaca agcgctcgag ccactgcccc
      601 acgcgtgcga gcgggacgcc gtcatgcgga gtgccgagaa ataccatggc gcgaagcttg
      661 cggggccaac cgtagcccgc cttggacgca tagtgatggg cgctgcgagc caccaagcct
      721 cccatgctgt gcgcgatgac gcatacctcc gcgagcggca cgggccaagc tgcggccaat
      781 gcgtcgagca tgttggcgaa ttcgcggccg ttcgtcgaca catgcaagcc gctgttgtag
      841 tgcaggtaga cggcggtaaa gctcagatcc cgctcgagcg aggcgccgtg atcgtggcct
      901 ttgcgcgtcc agcgcaggtc attcatgcag aggccgtgaa ccagcaccac taccttggtg
      961 ctcggctctc cgatcgacgc ggcaagggcc tcgcggttca actcgagcgg ctgtccgcgg
     1021 cggcgcagat gcatgctgat cgcgagtgag ttttcgctct tggcgaggta atcgccgagc
     1081 actccgttca acgccgcgac cagggcctcc cgggtgggcg aggagtcgag acggtgcagc
     1141 accgggccga tagcctccag cgcgcggtcg aggcctgcgc cgatgccccg cgtgacatcg
     1201 ccaatactct tgtagaccgc gtcggtgacg ccgttgatcg cctcgcccac gatggggccc
     1261 gcgaatcggg tgtatttctt ggcaatggcg ctgtgcaatg ccttcacgag atcggtgatg
     1321 ccggtgaccg cattgacggc cagtcgggac gcgccgcgaa gatcactcac cgagcacgca
     1381 gtccgccgct ttctcggcga tcatcatggt cggtgcattg gtattgccgc cgatcaggcg
     1441 cggcatgacg gaggcatcga cgacgcgcac gcccgacacc ccacggacac gcagctgcgg
     1501 gtcgacgacg gacttgaggt cgctgcccat gcggcaggtt cccgccggat gatagatcgt
     1561 ctcggcttta cgccgcacca tcgcctcgag gtcgccgcgg gtggttaccg tttctccggg
     1621 aaatatctcc gaaccccgaa aaggtgcaaa cggtcgcgaa ctgatgatct cgcgcgatac
     1681 gcgaatcccc tcgagcagca catccatgtc gcgggcgtcg ctgagatagg cggcatgaag
     1741 gcgcggcgga ttctgcggca gcgtcgagcg cagcgtcaag cggccccggc tgcgcggccg
     1801 cagcacgcac gcatggatgg tgaagccgtg accgtcaagc cggttacggc catgatcgtc
     1861 gagctgcgca ggcacgaagt gcagttgcac gtcggggcgc gcgtcaggcg ccagagatgt
     1921 gcgcacgaat gcacccgcct cggcgacatt gctgacgcca gggcccgagc gcgtcatgag
     1981 gtagcgcagt gcaacgacaa tctcctgcca ttgggtgaaa tcataggtaa ctggtcgagt
     2041 gcacttgttg agagtgcaga agtcgaggtg atcctgcagg ttgcccccga cctcagggat
     2101 gtcggcgagg acgggaattt tcaatgcccg cagatcatcg gccggccc
//
LOCUS       DASWZT010000343        12541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_50967, whole genome shotgun sequence.
ACCESSION   DASWZT010000343 DASWZT010000000
VERSION     DASWZT010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12541
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50967"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VGE92_09415"
     CDS             <1..381
                     /locus_tag="VGE92_09415"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEY1314086.1"
                     /translation="LLRMTFDFPQNDLKMAAYVTAKGVEIGKAMGAKQVAGSPRTGPY
                     TTTVYQTTHNTGGTVMGNDPRTSVVNRYLQSWDVPNVFVIGASAYPQNASWNPTDTLG
                     ALTYWAIDALKNRYLKNPGRLVSA"
     gene            411..1040
                     /locus_tag="VGE92_09420"
     CDS             411..1040
                     /locus_tag="VGE92_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY1314087.1"
                     /translation="MVCRLALGASVIFAASAFPAAGPESAKRAGPAAAVICQSCHGAN
                     GEGAPAARIPRLAGQSADYLDKQLRDYASGARSNEVMQNFAKPLSEADRAAVAAYFAS
                     LAAPFIAPNPPATDIQLARGRQLAQQGAEAERVQACDNCHGPDGSGLPHSAPYLAGQS
                     ADYLAAELQSWRQGKRKNDAGKLMSSVAQRLNDADVSAVSAYFSSLEAP"
     gene            1090..1491
                     /locus_tag="VGE92_09425"
     CDS             1090..1491
                     /locus_tag="VGE92_09425"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY1314088.1"
                     /translation="MRTLCQLSFVAVIALSLSAGADEPKPQPVRTLFSHHDQSGVPGK
                     EIVIGTAMLPPETAIGFHTHPGDESGYVLRGKLILKTRGQPDRILNPGDSFFNERGAI
                     HSLVAAPGGEGGTAVSTWIVDKGAPLATPVP"
     gene            complement(1648..2340)
                     /locus_tag="VGE92_09430"
     CDS             complement(1648..2340)
                     /locus_tag="VGE92_09430"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY1314089.1"
                     /translation="MGTTVSFNRPDGNAVSGYLAEPAHPAGAPAVVVIQEWWGVNDQI
                     RGVADRLASAGYLALVPDLYRGKATVEAEEAHHLMTGLNFADAASQDIRGAVQYLKSR
                     SVKVGVTGFCMGGALTLLSVCMAPEIDAGVVWYGCPPLEYIDATKIKAPLQAHWATQD
                     QFFPLATIDTLQDKLRAAGVNFDFHRYLAHHAFANETAVGPHRIPATQYDPVWSQQAW
                     DRSFRFFGRWLG"
     gene            complement(2493..3956)
                     /locus_tag="VGE92_09435"
     CDS             complement(2493..3956)
                     /locus_tag="VGE92_09435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY1314090.1"
                     /translation="MLRAKAVSWSAGLSLTLLLGACSLAPPLKTPVVPTADAYKEIGP
                     WTQAQPADRLPRDSWWALYGNAELDELEKRLIDGNPTLAAALANYAQARALADQARAG
                     LFPTLGLSANVERNRESINAPLRGPTTPTYYNANTVGGSVSYELDLWGQIRNEVAAGE
                     ANAAASAADLENARLSLIAQLADDYIQLRSLDRDTAILDQTVQAYAHALGLTQQRHGA
                     GIAPGLDVSQAQTQLDAARSQAAQTLAQRALMEHSIAALLGVSASTFSIKAEMVDIKL
                     PQIPTGLPSTLLERRPDIAGAQRRMMAANANIGIARAAYFPSLTLGAQGGFQSTSVAN
                     WLSAPSSFWAVGPNALLSVFDGGLRRAQVAQARAAFDASAAYYRITVVSAFQQVEDSL
                     ATLNHYHDASVDEKAAVDSAQRTLDFSLALYKQGATDYLTVVTSQTALLQTQLEALNL
                     DTLQLTASVDLIRALGGGWEDSAGAQNPRPLNARQRG"
     gene            complement(3943..5151)
                     /locus_tag="VGE92_09440"
     CDS             complement(3943..5151)
                     /locus_tag="VGE92_09440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY1314091.1"
                     /translation="MSQPDRIPSHPVPRRLTLLGGAFALLAIAVAAYGLGTRTAQDAR
                     LRDLTESQAAPVVALVTPASVKNDAGLDLPGRLQAYIQAPIYARVPGYLKSWKHDIGD
                     KVKAGDVLAEIDTPDLDQQLMQARADLSVAQANAKLAQISAERWQSLASTDAVAKQDV
                     DTRTFTWNANIAQVKSAQANVDHLAAEEGFKRLIAPFNGIVTARATDIGALINVGAAG
                     GAQLFVVSETGKLRVYVNVPQNYVPSVPPGTLATISVPEHPGKTYRGTVEASSQAVDP
                     TSGTTLMQLIVDNSAGEMMPGDYASIHLQIAAAAHVLSVPSSALIFDAKGLSIATVDS
                     TNRVLLKPVSIERDLGAVVELASGLATNDRVIENPPDGIGNGDQVRLAGAAAAGALAH
                     AKPKNKNAAG"
     gene            complement(5138..8335)
                     /locus_tag="VGE92_09445"
     CDS             complement(5138..8335)
                     /locus_tag="VGE92_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1314092.1"
                     /translation="MLAIVRIALSRPYTFIVAALLILLMGTLAASRMPVDIFPSINIP
                     IIGVAFQYQGLPPDQMAGRIITPFERALTTTVNDIEHFEANSYTTFGIVKIYFHPTVN
                     IATANAQVTAISQTLLKQMPAGTTPPLIINYSASSVPIIQLALSGKGLTEQSLADLGV
                     NFVRPRLVTVQGAGIPWPFGGKSPQVMFDLDTAALESRGLTGLDVANALAAQNLITPV
                     GTEKIGNDEYTVNLNSAPSKISELGDLPIKNVGGAMVYMRDVAHVRNGSAVQTNVVHV
                     DGNRSVLISVFKNGATSTLDIVSGIKKMLTIIKPSLPDALDIKPIGDQSIFVRASIQG
                     VIREGVIAAALTSLMILLFLGSWRSTLIVTTSIPLAILGAIGALSAIGETLNIMTLGG
                     LALAVGILVDEATVTIESINYHLEQGKEVEAAILDGASQIVIPAFVSLLCICIVFIPM
                     FFLSGVARFLFVPMAESVIFAMVFSFLLSRTLVPTMAKYLLRMHARHAGGKDSEPPTT
                     TNPLVRFQLGFEAAFARLRTGYHDLLALAIERRRLFLGGFAGFIALSFLLAPFLGRNF
                     FPQVDGGQILLHVRTPVGMRIEETAARFAEVEKAIREVIPPAEVAAVADNIGTYLSSI
                     NTIYNNTGTIGESDGDIQISLSPGHAPTADYVSRLRDVLPRSFPGTTFSFLPADIVSQ
                     ILNFGAPAPIDLQVRGNDLGADYYFANKLLAQVRHVPGVVDARIQQSARQPTIDVNMD
                     RTRGQYTGVTAADVTNSLVVNLASSSQVAPTYWLNEKNGVTYPIALQTPQYQIDTLPA
                     LQNLPINATGAPTTVLGGIADIRRSHSDAVVSQYDIAPMVEIYASTQGRDLGAVSADI
                     RKIMARNDNDKPKGSIVALVGQTATMNSAYSGLLLGLLGAIVLVYFVVVINFQSWSDP
                     FVIITALPAAIAGIVWMLFATHTTLSVPALTGAIMCMGVATANSVLVISFAREKFIET
                     GDATQAALEAGFVRLRPVLMTALAMVIGMLPMALGLGEGGEQNAPLGRAVIGGLIFAT
                     VSTLFFVPVMFSIIHRHRAPKPAPATHFGAAHVPA"
     gene            complement(8340..9359)
                     /locus_tag="VGE92_09450"
     CDS             complement(8340..9359)
                     /locus_tag="VGE92_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY1314093.1"
                     /translation="MSASIQVHATPVETEFVEIPATGLKVSRVALGTWAMGGWMWGGT
                     DQHESIATIHAALSQGINLIDTAPVYGFGVSEEIVGAALGGVRTQVVIATKAGLEWRD
                     GKIYRNATRTRIMQEIDASLRRLRTDYIDIYQVHWPDPLVPMEETAEAMRSLYEQGKI
                     RAIGVSNFSVAQMESFRQVAPLHVLQSPYNLFERAIEADILPYCRANHIVTLGYGALC
                     RGLLSGRMRPDTKFEGDDLRRIDPKFQPPRFAQYIGAARQLDELAQRRFRRRVIHLAV
                     RWMLDKGIDVALWGGRHPDQVEAALGIAGWSLNGADRELIERIVNAAVTDPVGPEFMA
                     PAQRG"
     gene            complement(9442..9852)
                     /locus_tag="VGE92_09455"
     CDS             complement(9442..9852)
                     /locus_tag="VGE92_09455"
                     /inference="COORDINATES: protein motif:HMM:NF016834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lecithin retinol acyltransferase family protein"
                     /protein_id="HEY1314094.1"
                     /translation="MPTVWPSGLDREPPVGAHLATSRRGYTHHGMYVGRGRVVHYSGL
                     SGFWQCGPVEEVSLSCFTAGHSARIVDHERSMYSPQEIVRRARSRIGENDYRLLTNNC
                     EHFCNWCLYGVSRSAQVERRWQLPWRVLIALFQT"
     gene            complement(9892..10038)
                     /locus_tag="VGE92_09460"
     CDS             complement(9892..10038)
                     /locus_tag="VGE92_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314095.1"
                     /translation="MNTIRMFAFAAAILITAALFAVIADSFESETPVDAATAAHAPAD
                     RASP"
     gene            complement(10148..11155)
                     /locus_tag="VGE92_09465"
     CDS             complement(10148..11155)
                     /locus_tag="VGE92_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY1314096.1"
                     /translation="MSSEAIMYRMMRYSFAIATSICAVMLAGERAFGEPRIIREAPNG
                     DLFVADSRLDALRVLRVSRGRATPLRDAVFARGLKHPLGIAFYPVGSDPRWIYIANSS
                     GLVRFRYRNGDLKATSKPEQIIAGVPTIHQYARGVVFSPDGRRLHVSVGSARALDATG
                     LRTCLGMTVQPATGELSCIVDEHDESSDDTAQSALLQMGVYQGDHASRAYTPTAVIAS
                     ESVQNGDDAARSRDLYLAHCAACHQASGEGLPGVFPPLKGSGVVNKDDASKHIQVILN
                     GMQGGRAGGVVYAAPMPPFADTLSDAQIASIINYERSSWGNHGMPVTAARVAAERAPP
                     K"
     gene            11343..11852
                     /locus_tag="VGE92_09470"
     CDS             11343..11852
                     /locus_tag="VGE92_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEY1314097.1"
                     /translation="MNARESDHLMLGTLPTSPGLDSRAVADIASALTVILADMFALYI
                     KTKNFHWHVSGPHFRDYHLLLDEQGDQIFAATDAVAERVRKIGGTTLHSIGHIARLQR
                     IADNDEGYVAPANMLEELMDDNRRLVVSLRAAHALCDDAGDVAGASLIEIWIDEAERR
                     AWFLYEATR"
     gene            complement(11881..>12541)
                     /locus_tag="VGE92_09475"
     CDS             complement(11881..>12541)
                     /locus_tag="VGE92_09475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1314098.1"
                     /translation="HCTLPAGAYVRLRVSDTGSGIPPHVLERMFDPFFTTKGTGKGTG
                     LGLSLVHGIVADLGGGVDVSTVVGLGTTFTIWLPCSGEAAAPSTEPTARLPRGRGQTI
                     LIVDNEEPLVALAEETLAELGYEPIGFSSSIVALQAFREAPWRFDIVLTDESMPELSG
                     TDLAREIVLLRPDIPIVLMSGFGGSQLHERAQAVGIRELLRKPLQKKDLADCFERVLA
                     G"
BASE COUNT         2349 a         3783 c         4076 g         2333 t
ORIGIN      
        1 ctgctgcgca tgaccttcga ttttccgcag aacgatttga agatggcggc ctacgtcaca
       61 gccaagggtg tcgagatcgg caaagccatg ggcgcgaagc aggtcgccgg ttcgccgcgc
      121 acgggcccct atacaacgac ggtgtatcag acgacgcaca acacgggcgg cactgtgatg
      181 ggcaacgatc ctcgcacgag cgtcgtcaat cgctatctgc aatcctggga cgtcccgaac
      241 gtgttcgtca tcggagcatc ggcttatccg cagaacgcgt cctggaatcc gaccgataca
      301 ttgggtgcgt tgacgtattg ggcaatcgac gcccttaaga atcggtacct caagaatccc
      361 ggtcgcttgg tgtccgcgtg acacgcgtca tgcaccccct ctcatctgcc atggtctgcc
      421 ggctggcgct tggagcgagc gtcatcttcg ccgccagcgc tttccctgcg gccggacccg
      481 agtcggcaaa gcgagcgggt cccgctgcgg cggtgatctg tcagagctgt cacggtgcga
      541 acggggaggg ggccccggcg gctagaattc ctcgactcgc ggggcagtcg gcagactatc
      601 tcgacaagca actgcgcgac tacgcctctg gcgcccgcag caacgaagtc atgcagaatt
      661 tcgccaagcc tctgagcgag gcggatcgcg ccgcggtcgc cgcgtatttt gcatcgctcg
      721 cggctccctt cattgcacca aatcccccgg ccactgacat ccagttggcg aggggccgtc
      781 aattagccca acaaggagca gaggcagagc gcgtacaggc ctgtgataac tgtcacggac
      841 ctgacgggag cggcctgccc cattcggcgc cttacctggc cgggcaatca gctgactatc
      901 tcgccgccga actgcagtcg tggcggcagg gcaagcgcaa aaacgatgcc ggcaagctca
      961 tgtcctcggt ggcgcagcga ctgaacgatg ccgatgtttc ggctgtttca gcctatttct
     1021 cgagtctcga agcaccatag aacccatacg tagcggtgag agtttcactc agcgatcagg
     1081 agcacagcca tgcgcacact atgtcaattg tcctttgttg cagtcatcgc gctgtcgctc
     1141 agtgccggcg ccgacgagcc taagccacag cccgtgcgta cgctgttctc gcatcacgac
     1201 caatcggggg tacccggcaa agagatcgtc atcggcactg cgatgctgcc gcccgaaacc
     1261 gcgatcggct ttcatacgca tcccggcgat gagagcgggt acgtcctcag agggaaactg
     1321 atcttgaaga cacggggtca gccagatcgg atactcaatc cgggggacag cttctttaat
     1381 gagcgaggcg caatccatag cttggtcgct gcacccggag gagagggcgg cacggccgtg
     1441 tcgacctgga tcgtcgacaa aggagcgccc ctcgcgacgc cggttccgtg acgagcgcac
     1501 caatgagcgc ggatcagcga atcgccgatg ttgcgacggc gggccatgcc gatttcgaca
     1561 tcgtcaattc cgatcaattc atgtcaaggg tggtgtcgtg acggatttga cgcgcagatt
     1621 catgctatga agtagtaacg ccatccgtca gccgagccag cgtccaaaga acctgaagga
     1681 ccggtcccat gcctgctgcg accacacggg gtcatattgg gtggcgggaa tgcggtgcgg
     1741 ccctacggcg gtctcgttgg cgaaggcatg atgtgccaga tagcgatgga aatcgaagtt
     1801 caccccggca gcgcgcagct tgtcctgcag cgtgtcaatt gtggcgagag gaaagaactg
     1861 atcctgcgta gcccaatgcg cctgcagcgg cgccttgatc ttggtcgcgt cgatgtactc
     1921 gagcggcggg cagccgtacc agacaacgcc cgcgtcgatc tccggggcca tgcacacgct
     1981 gagcagcgtc agggcacccc ccatgcaaaa cccagtcacg cccaccttga cgctgcgcga
     2041 cttgaggtat tgcacggcgc cgcggatatc ctgagacgcg gcatcggcga aattgaggcc
     2101 ggtcatgaga tgatgtgctt cttcggcctc aaccgtggcc ttgccgcggt acaggtcggg
     2161 taccagggcg aggtaccccg cactggccaa tcggtcggcg acgccacgaa tttggtcgtt
     2221 gactccccac cactcctgga tgacgacgac tgccggcgca ccggcaggat gtgccggctc
     2281 ggcaagatag cccgacacgg cgttgccgtc cgggcgattg aaagagactg tcgtgcccat
     2341 ggcttcctct attgtggaga tgatcagatg ttgggcgcga gttgcgcatg aagccgacac
     2401 taccggagat tgaattcctg tcaatccatc cctacggacg agcccctgct cggcgctctg
     2461 ttccggcaga ccgcatcggg gtacgcgagc tgttatccgc gctgcctcgc gttcagcggc
     2521 cgcggatttt gtgcgcccgc ggaatcctcc cagccgccgc cgagcgcccg gatgaggtcg
     2581 acgctggcgg tcagctgcag cgtatcgagg ttgagcgcct cgagctgagt ttgcaggagc
     2641 gcggtttgcg aggtgacgac ggtgagatag tcggtcgcac cctgcttgta aagcgccagg
     2701 gagaagtcca gggtgcgctg tgcggagtcg acggcggcct tttcatcgac gctggcgtcg
     2761 tggtagtgat tgagtgtggc gagactgtct tcgacttgct gaaatgcgct cacgacggta
     2821 atccggtagt acgcggcgga ggcatcgaac gccgcgcgcg cttgggcgac ctgcgcacgt
     2881 cgcaggccgc cgtcgaagac actgagcagg gcgttaggtc ccacggccca gaacgagctc
     2941 ggcgcgctca gccagttggc gacgctggtg ctctggaagc cgccctgagc gccgagcgtg
     3001 agcgatggaa aatacgccgc gcgcgcgatc ccgatattgg cgttggcggc catcatgcgc
     3061 cgctgcgcgc ccgcgatatc cgggcggcgc tcgagcaaag tggaaggcag gccggtggga
     3121 atctgcggca gcttgatgtc gaccatctcg gccttgattg agaacgttga agctgacaca
     3181 ccgagcagcg cggcgatcga gtgctccatc agcgcgcgct gagcgagcgt ctgtgcggcc
     3241 tgggagcgtg cggcatcgag ttgagtctgt gcctgcgaca cgtcgaggcc cggtgcaatg
     3301 ccggccccgt ggcgctgctg ggttagccca agcgcgtgcg cataggcctg caccgtctga
     3361 tcgaggatgg cagtgtcgcg gtcgaggctg cgcagttgaa tgtagtcatc ggccagctgc
     3421 gcgatcagtg acaggcgagc gttctcgagg tccgcggcgg acgcggccgc attggcttcg
     3481 ccggcggcaa cctcattgcg aatttgcccc cacagatcga gttcatagct gacactgccg
     3541 ccgaccgtgt tggcgttgta gtaggttggc gtggtggggc ccctcagcgg cgcgttgatc
     3601 gattcgcgat tgcgctcgac gttggcgctc aagcccagcg tgggaaagag ccccgcgcgc
     3661 gcctgatcgg caagcgcccg ggcctgcgcg tagtttgcca gtgcggcggc gagagtcggg
     3721 ttcccgtcga tcaaccgttt ttcaagctcg tcgagctccg cgttgccata cagtgcccac
     3781 cagctgtcgc gcggcagccg gtcggccggc tgtgcctggg tccagggccc gatctcctta
     3841 taagcatccg cagtgggaac gaccggggtt ttcaacggcg gcgcaagcga acacgcgcca
     3901 agcagcagtg tcagcgagag gcccgcggac caagatacgg ccttagcccg cagcattttt
     3961 attcttcggc ttagcatggg cgagcgcgcc ggcagccgct gcgccggcga ggcgcacctg
     4021 gtcgccattg ccgattccat cgggcgggtt ttcgatcacg cggtcgttcg tcgcgaggcc
     4081 ggaggcaagt tcgacgaccg cacccaggtc gcgttcgatc gacaccggct tgagcagcac
     4141 gcggttcgtc gagtccacgg tggcgatgga gagccccttg gcgtcgaaaa tcagcgcgct
     4201 cgatggcacg ctgagcacat gggcggcggc ggcaatttgc agatggatgc tggcgtagtc
     4261 gcccggcatc atctcacccg cgctattgtc gacgatgagc tgcattagcg tcgtgccgct
     4321 ggtcgggtcg acggcctggg acgaagcctc caccgttccg cgataggtct tgccgggatg
     4381 ttccggcacg ctgatcgtgg ccagggtgcc cggcggcacg ctggggacat agttctgcgg
     4441 cacgttgaca tagacgcgga gcttccccgt ttcggagacc acgaacagct gcgcgccgcc
     4501 ggcggccccg acgttgatca gggcgccgat gtcggttgcg cgcgcggtga cgatgccgtt
     4561 gaacggcgcg atcaagcgtt tgaagccttc ctcggcggcc aggtggtcga cgttcgcctg
     4621 cgccgacttg acctgcgcga tattggcgtt ccaggtgaac gtgcgcgtat cgacatcttg
     4681 cttggcgaca gcgtcggtgc tggcgagcga ctgccaccgc tcggcactga tctgcgcgag
     4741 cttggcattg gcctgtgcga cgctcaagtc ggcgcgcgct tgcatcagtt gctggtcgag
     4801 atcgggcgta tcgatttcgg cgagcacatc gccggccttc accttgtcgc cgatatcgtg
     4861 cttccagctt ttcaggtaac ccgggacgcg ggcgtaaatg ggcgcttgga tataggcttg
     4921 caggcgccca ggtaagtcca agcccgcgtc attcttgacg cttgcgggtg tcacgagcgc
     4981 cactaccggt gcggcttgcg attcggtcaa atcgcgaagg cgggcgtctt gcgccgtgcg
     5041 ggtgccgagg ccgtaagcag caacggcaat ggcgagaagc gcgaatgccc ccccgagcag
     5101 ggtgaggcgt cgcggaacag ggtggctggg aattcgatca ggctgggaca tgggctgctc
     5161 cgaagtgggt cgccggcgcc ggctttgggg cgcggtgccg gtggataatg ctgaacatca
     5221 caggaacgaa gaagagcgtc gagacggtgg cgaagatcag gccgccgatc acggcgcggc
     5281 cgagcggcgc attctgctcg ccgccttcgc ctaacccaag cgccatgggc agcatgccga
     5341 tcaccatggc gagtgccgtc atcaacaccg gacgcaggcg cacgaatccg gcctcgagcg
     5401 cggcttgtgt cgcatcgccc gtctcaatga acttctcgcg cgcgaagctg atcaccagca
     5461 cgctgttcgc ggtcgcgacg cccatgcaca tgatggcgcc tgtgagagcc ggcaccgaca
     5521 gcgtggtatg cgtcgcaaac aacatccaga cgatgccggc gatcgccgcc ggcagtgcgg
     5581 tgatgatgac gaacgggtcg ctccacgact ggaaattgat cacgaccacg aaatacacca
     5641 gtacgatggc gccaagcagg ccgagcagca ggcccgagta ggcgctgttc atggtcgccg
     5701 tctgaccgac cagggcgacg atcgatcctt tcggcttgtc gttgtcgttc ctggccatga
     5761 tcttgcggat atcggcggag actgcgccga gatcccgccc ttgagtcgat gcgtagatct
     5821 caaccatcgg agcgatgtcg tactgcgaga ccaccgcgtc gctgtggcta cggcggatgt
     5881 cggcgatgcc accgagcacg gtggtgggcg cgccggtggc gttgatgggc agattctgca
     5941 gggccgggag cgtatcgatc tgatattgcg gagtctgcag ggcgatcgga taagtaacgc
     6001 cgttcttctc gttgagccag taggtgggcg ctacctggct ggaactcgcc aggttgacga
     6061 ccaggctgtt ggtcacgtcg gccgcggtca cgccggtgta ttggccccgc gtgcggtcca
     6121 tgttcacatc aatcgtcggc tgccgggccg actgctggat gcgcgcatcg acaacgcccg
     6181 ggacatgtcg aacctgtgcc aggagcttat tggcaaaata atagtccgcg cccagatcgt
     6241 tgccgcggac ctgcagatca atgggcgccg gcgcgccgaa gttcagaatc tggctgacga
     6301 tgtcggcggg caggaacgag aacgtcgtgc cggggaaact gcggggcagc acatcgcgca
     6361 aacgactgac atagtcggcg gtcggcgcat gccccgggct gagcgagatc tggatgtcgc
     6421 catcggattc gccgatcgta ccggtattgt tgtagatcgt attgatcgac gaaagatagg
     6481 tgccgatgtt gtccgccacc gccgccactt cggccggggg gatcacctcg cggattgcct
     6541 tctccacctc ggcgaaacgc gcggcggtct cctcgatacg catcccgaca ggcgtccgta
     6601 catgcagcag tatctggccg ccgtcgacct gagggaagaa attacggccg agaaatggcg
     6661 caagcaggaa tgagagcgcg atgaagccgg caaaccctcc caagaacaac ctgcgacgct
     6721 cgatggcgag ggcgagcaga tcgtgatacc cagtgcgcag ccgtgcaaag gccgcctcga
     6781 agccgagctg aaaacgcacc agtgggttcg tggtggttgg cggttcagaa tccttgccgc
     6841 cggcgtgcct cgcatgcatc cgcagcaaat acttcgccat cgtcggcacc agcgtacgcg
     6901 acagcaagaa cgaaaagacc atggcgaata tcacgctctc cgccatcggc acgaacagga
     6961 agcgcgcgac gccgctcaga aagaacatcg gaatgaacac gatgcagatg cagagcaggg
     7021 acacgaatgc cgggatgacg atctgggagg cgccgtcgag gatcgcggcc tccacctcct
     7081 ttccctgttc gaggtggtag ttgatgctct cgatcgtcac cgtcgcctcg tcgacgagga
     7141 tgcccacggc gagggcgagc cctcccaagg tcatgatgtt gagtgtttcg ccgattgccg
     7201 agagtgcgcc gatcgcgccg aggatcgcca gcggaatcga ggtggtcacg atcagggtcg
     7261 agcgccagct gccgaggaac agcaggatca tcaagctcgt gagcgcggcc gcgatcacgc
     7321 cctcgcggat cacgccttgg attgaagcgc gtacgaatat cgactggtcg ccgatgggct
     7381 tgatgtcgag ggcgtccggc agcgagggct tgatgattgt cagcatcttc ttgataccgc
     7441 tgacgatgtc gagtgtcgag gtcgcaccat tcttgaacac gctgatcagc accgagcgat
     7501 tgccgtccac gtgcacgaca tttgtctgta ccgcactgcc gttgcgcaca tgggcgacgt
     7561 cgcgcatgta gaccatcgcg ccccccacgt tcttgatcgg taggtcgcca agctccgaga
     7621 ttttcgacgg cgcgctgttg aggttgacgg tgtattcgtc attgccgatc ttttccgtgc
     7681 ctaccggggt gatcaggttc tgtgcggcca gcgcattggc gacgtcgagc ccggtcaggc
     7741 cgcgcgactc gagcgctgcg gtgtcgagat cgaacatcac ctgcggcgac ttgccgccga
     7801 acggccaggg aatgcccgcg ccctgcaccg tgaccaggcg cgggcgcacg aagttgacgc
     7861 cgaggtcggc gaggctctgc tcggtcagcc ccttgcccga cagcgcgagc tggatgatcg
     7921 gcaccgacga ggcgctgtaa ttgatgatca ggggcggtgt cgtgccggcc ggcatctgct
     7981 tgagcagggt ctgcgatatc gcggtgacct gcgcgttggc ggtggcaatg ttgacggtgg
     8041 ggtggaagta gatcttgacg ataccgaagg tggtatagga attcgcttcg aagtgctcga
     8101 tgtcattgac cgtggtcgtc agcgcgcgct cgaatggagt gatgatgcgt ccggccatct
     8161 ggtcgggcgg caagccctgg tattggaagg ccacgccgat gatcgggata ttgatgctcg
     8221 gaaatatatc gaccggcatc cgcgacgcgg cgagcgtgcc catgaggaga atgagcaggg
     8281 cagctacgat aaacgtatag ggccggctca gcgcgattcg gactatggca agcacgtaat
     8341 cagccgcgtt gcgcaggcgc catgaactcg ggtccgaccg gatcggtgac cgccgcgttc
     8401 acgatgcgtt caatcagctc gcggtcggcg ccgttgagcg accagccggc gatgccgagc
     8461 gccgcctcca cttgatcggg gtgacggccg ccccagagtg cgacgtcaat gcccttgtcg
     8521 agcatccagc gtaccgcgag gtgaatcaca cgccgccgga agcggcgctg cgcgagctca
     8581 tcgagctggc gggcggcgcc gatatattgg gcaaagcggg gcggctggaa tttggggtcg
     8641 atgcgccgca aatcgtcgcc ctcgaacttg gtgtccggcc gcatgcgccc gctcagcaaa
     8701 ccccggcaga gtgcaccata gccgagtgtg acgatgtggt tggcgcggca gtagggaagt
     8761 atgtccgctt cgatggcgcg ctcgaataga ttgtagggcg actgcagtac gtgcagaggc
     8821 gcgacttgcc ggaaactctc catctgcgcg accgagaaat tgctgacgcc gatggcacga
     8881 atcttccctt gttcgtaaag cgagcgcatg gcttcggcgg tttcctccat cggcaccaga
     8941 ggatcgggcc agtgaacctg gtagatatcg atataatcgg tgcgcagacg gcgcagcgag
     9001 gcgtcgatct cctgcatgat ccgggtgcgg gtcgcatttc gatagatctt gccgtcgcgc
     9061 cattccagtc ccgccttggt ggcaatgacg acctgagtgc gcacgccgcc gagagctgcg
     9121 ccgacgattt cctcggatac gccgaagccg tagaccggcg ccgtatcgat gagattgatg
     9181 ccttgagaaa gcgccgcatg aatggtggcg atcgattcgt gttgatcggt accaccccac
     9241 atccaaccgc ccatcgccca tgtaccgagg gccacccgcg ataccttcag gccggtcgcc
     9301 gggatctcca cgaattcggt ttcgacgggg gttgcatgga cttgaattga cgctgacatt
     9361 ggatcgagtc tcctgattga tcgactgcgt acagcatgga cgcgaaggat ttattgggtg
     9421 ttgcagaatc cgccgccacg attacgtctg aaacagggcg atgagtaccc gccaaggcag
     9481 ctgccatcgc cgctcgactt gtgcactgcg gctgacgccg tagaggcacc agttgcagaa
     9541 gtgctcgcaa ttattggtca gcaggcgata gtcattctcg ccgatgcgtg agcgggcgcg
     9601 tcgcacgatc tcctgcggcg aatacattga gcgctcgtga tcgacgattc gcgccgaatg
     9661 accggcggtg aagcaagaca gcgacacctc ctcgaccggg ccgcattgcc agaatcccga
     9721 gagacccgag taatgcacca cgcgaccgcg gccgacatac atgccgtgat gcgtgtagcc
     9781 gcggcgcgag gttgcgagat gggcgccaac aggcggctcc cgatcgagtc cggatggcca
     9841 cactgttggc atgtcatcca tctgccctga tccgcgctgt cttggaaacg gtcaggggga
     9901 agcgcgatcg gctggcgcgt gagcggccgt cgcagcatcg accggagtct cggattcgaa
     9961 gctgtccgcg attacggcaa acagagcggc cgttatcaag atcgctgcgg caaaggcaaa
    10021 cattcgaatg gtattcatgg gatttcctcg agtgtggtta attgctgatg tcgcgtgcaa
    10081 tttactcacc gcttgttgac tcgctgtttc ctgcgggcgc gccgccgttt caggtgaaac
    10141 gcgctgctca cttgggaggg gcgcgttcgg cggccactcg cgctgccgtc accggcatgc
    10201 cgtgatttcc ccaggagctg cgctcatagt tgatgatgct ggcaatctgc gcatcgctga
    10261 gcgtgtcggc aaacggcggc atgggcgcag cgtagacgac gcctcccgcc cggccgccct
    10321 gcataccatt gagaatgacc tgaatgtgtt tcgaagcgtc gtctttattg acgacgccgc
    10381 ttcccttgag gggcggaaac acgccgggga gaccttcgcc actggcttga tggcaggccg
    10441 cgcagtgggc gaggtagagg tcccgactcc gcgccgcatc gtcgccgttc tgcacggact
    10501 cgctcgcaat gactgccgtc ggtgtgtacg cgcgcgaagc gtggtcgccc tgataaacgc
    10561 ccatctgcag gagcgccgat tgcgccgtgt catcactcga ttcgtcgtgc tcgtcgacga
    10621 tgcacgagag ttcgccggtg gccggttgga ccgtcatgcc taggcacgtc cgcagccccg
    10681 tcgcgtcaag cgcgcgcgcg gatcccacgg aaacgtgcag ccgacgtcca tccggcgaaa
    10741 aaacgacgcc gcgagcgtat tgatggatcg tcgggacccc ggcgatgatc tgctccggtt
    10801 tgctggttgc cttgaggtcc ccatttctat agcgaaaccg cacaaggccg ctgctgttgg
    10861 cgatgtagat ccagcgtggg tcggatccaa cggggtagaa cgcaatgccg agcggatgct
    10921 tcagcccacg ggcaaacact gcatcgcgca gcggcgttgc gcggccccgg gaaacacgca
    10981 atacacgtag cgcgtcgagc cgcgaatcgg cgacgaacag atcaccgttg ggcgcttcgc
    11041 ggatgatccg cggctctcca aatgcacgct cgccggcgag catcaccgca caaatggaag
    11101 tcgcgatcgc gaacgagtaa cgcatcattc gatacatgat tgcctccgaa ctcatttgaa
    11161 atgtctgccc gcagtcatga ggccatcgtt ggattcagaa tcgatttcga tgcggctacc
    11221 ggtgaaatca aatcgaccag gctcgcatgt catttgaaat atgcggatcg accattgaaa
    11281 tacagcggta gggcaccgcg ccgatagtca cacacacccg aaatctagag gagattgcaa
    11341 cgatgaacgc tcgcgagtcg gaccatctaa tgcttgggac gctgcccaca tcaccgggct
    11401 tggactcccg cgccgtcgcc gatattgctt ccgctttgac ggtcatcctg gccgacatgt
    11461 tcgcccttta catcaagacg aagaactttc attggcatgt ctcgggcccg cacttccgcg
    11521 actatcatct tctcctcgac gagcagggtg atcagatatt cgccgcgacc gacgcggttg
    11581 cagagcgggt gagaaagatc ggcggaacga ccttgcattc gattggacac atcgcgcgct
    11641 tgcagcgcat tgccgacaac gacgagggat atgttgcgcc cgcgaacatg ctcgaggaac
    11701 tcatggacga taatcgacga ttggttgtca gcctgcgagc ggcgcacgcg ctttgtgatg
    11761 atgccggcga tgttgccggg gcgagcctga tcgagatttg gatcgacgag gcggagcgtc
    11821 gcgcgtggtt tctatacgag gccactcgct gagcgtcgca attcgaatcg accggccgtc
    11881 tcagccagcc aagacgcgct cgaaacagtc cgcgaggtct tttttctgca ggggcttgcg
    11941 cagcagctcg cgaattccga ccgcctgcgc gcgctcgtgc aattgcgagc cgccgaaacc
    12001 gctcatcagt acaatcggta tgtcaggccg gagtaacaca atttcgcggg caagatcggt
    12061 gccgctgagt tccggcatcg attcgtcggt caggacgata tcgaagcgcc acggtgcttc
    12121 gcggaacgcc tgcagtgcga cgatgctgga actgaatccg atgggctcat agccgagctc
    12181 cgccagggtt tcctcggcga gcgcaaccag cggctcttcg ttgtcgacga tgagtatcgt
    12241 ttggccgcga ccacgcggca gccgggccgt tggttcagtc gatggcgccg ccgcttcgcc
    12301 gctgcacggc aaccagatcg tgaaagtcgt acccaagccg acgaccgtgc tgacgtcgac
    12361 cccgccgccg agatccgcca caatgccgtg caccaacgac agacccagac ccgttccctt
    12421 gccggtgcct ttggtggtga agaacggatc gaacatccgc tccagcacat gcggcgggat
    12481 tccacttccc gtgtcgctca cgcgcagccg cacgtaggcg ccggcgggca gagtacagtg
    12541 t
//
LOCUS       DASWZT010000344         3187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_7503, whole genome shotgun sequence.
ACCESSION   DASWZT010000344 DASWZT010000000
VERSION     DASWZT010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7503"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1560
                     /locus_tag="VGE92_09480"
     CDS             <1..1560
                     /locus_tag="VGE92_09480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY1314099.1"
                     /translation="VGDGGWQLAAPRDAALRGSWWEIYGDATLNDLEARVVISNQTVI
                     AAEANYRAAQALVQQSQAALFPTVSTDPSVTRSKSSASVLSSSNSGTTATGSSTPSTG
                     TSTGAGSSVNTSAGGSTSPRTIFALPLEATYQVDLWGSIRNTVAQNRYSAQASAAQLA
                     NALLSTQTSLAQDYFQMRIADEERRILDDTLADFRANRRLVQTLFNNGLDSDEDLAQA
                     DTQLKSAEAQATDLGITRATYEHAIAVLIGVPPSKFSLPYKRFNQTLPDVPVDGIPAD
                     LIERRPDIAAAERQVAAANAAIGVARAAYFPALTVSGTAGYESTALGQLLNAPNRFWS
                     FGPDLAETLFDGGARRAASARARAEDDAAAANYRQTVLSALQSVEDNLASLRILAQEV
                     AEEHAAAVAAQRAVQLSVVRFRNGVDSYVNVITAQNAFLSARETEVQVELRQLTASVN
                     LINNLGGGWSKAQLPQTESLGQNPARANSEPNVPIEASEHVSPNPPPLPAREILPEEM
                     LEQDQETLGKP"
     gene            complement(2422..>3187)
                     /locus_tag="VGE92_09485"
     CDS             complement(2422..>3187)
                     /locus_tag="VGE92_09485"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY1314100.1"
                     /translation="PRLADAAQTQGADVEIKTADGTCDAYFVSPVTGKYPAVLTWPDI
                     FGLRPASKEMATRLASSGYAVLVINPFYRIKKAPTAPEHPDFNDPATRQALLSMAGTL
                     SAATAATDAKAFVAFLDAQPAVDKKKKMGTTGYCMGGPFVFRTAAAYPDRVGAAATFH
                     GGSLVTDKPDSPHLLIPQMKARYLVAIAENDDARQPEAKTVLKDAFAKAHLTAEVEVY
                     AGAQHGWCPPDSAVYNHDQAEKAWSRQLALFSKALV"
BASE COUNT          545 a         1006 c         1009 g          627 t
ORIGIN      
        1 gtcggcgatg gcgggtggca gctggcggca ccgcgcgatg cggcgctgcg cggcagctgg
       61 tgggagatct acggcgacgc gacgctgaac gatctggagg cgcgcgtcgt gatttcgaat
      121 caaaccgtga ttgcggcgga agcgaactac cgggcggcgc aggcgctcgt acagcagtcg
      181 caagcggcgt tgtttccgac cgtcagcacc gatccatccg taacgcggtc caagtcatcc
      241 gcctcggtgc tctcttcttc caattccggc actaccgcga ccggcagcag cacgccgagc
      301 accggcacga gcactggcgc gggtagttcc gtgaacacct ccgccggcgg ctcaacctcg
      361 ccgcggacga ttttcgcgct gccgctcgag gccacctatc aagtcgactt gtggggcagc
      421 atccgcaata ccgtggcgca aaatcgctat tcggcgcagg cgagtgccgc gcagcttgcg
      481 aacgccttgt tgagtacgca aaccagcctc gcgcaggatt actttcagat gcgcatcgca
      541 gacgaggagc ggcggatcct ggatgatacc ttggccgatt ttcgagccaa ccgccgcttg
      601 gtgcaaaccc tgttcaacaa cgggctcgat tccgacgagg atctcgcgca ggcggatact
      661 caattgaaat ccgccgaagc ccaggcgacg gatcttggga tcacgcgcgc cacctacgag
      721 catgcgatcg cggttctgat cggcgtgccg ccctcgaagt tctcactgcc ctataagcgc
      781 ttcaatcaaa cactgccgga cgtgcctgtc gatggaatcc ctgcggattt gatcgaacga
      841 cgtcccgata ttgcggccgc ggagcgtcag gtcgcggccg caaacgcagc cattggagtt
      901 gcacgagctg cctacttccc ggccctgacg gtcagcggca ccgccggcta tgagtcgacc
      961 gccttgggtc aactgctcaa cgcgccgaat cgattctggt cgttcggacc cgacctcgcc
     1021 gagacgttat tcgacggcgg cgcgcgccgc gctgccagcg cccgagcacg tgccgaagac
     1081 gatgcggccg cggcgaatta ccgccagacg gtgctgagcg cgctgcaatc ggtggaagat
     1141 aacttggcca gtctgcggat tctcgcgcaa gaggtcgccg aagagcacgc agcggccgtc
     1201 gccgcgcagc gcgcggtgca actctccgtg gttcgatttc gtaatggcgt cgacagctat
     1261 gtcaatgtca tcaccgcgca gaatgcgttc ctttcagctc gcgaaacaga agtgcaggtc
     1321 gagctgcgcc agcttaccgc cagcgtcaat ttgatcaaca atttgggtgg cggctggtcc
     1381 aaagcgcagc tgccgcagac cgagtcgcta ggccaaaacc cggcccgcgc gaacagcgag
     1441 ccgaatgtcc ccatcgaggc ttccgaacac gtctcgccca atcccccgcc tctgccggcg
     1501 cgtgaaatcc tccccgaaga gatgctcgag caagaccagg agaccctcgg gaaaccctaa
     1561 catgaggtgt tgcccatgct tggtgctatc gtagggtagg gcatgcgcac agaacctcaa
     1621 cgggatcgag cgatgcgtat cgccggcgcg ggacgtgcga tctttgcgac gactctgatt
     1681 gtgctcggag tcctgggtat tttaaaaggc aatttcactc cgccgtggca gccggtaccg
     1741 aagggcgtgc ctgcacgcga cgtgctggtg tatatgtgcg ccttcatctg cctcgggtgt
     1801 ggcctcggct tgctctggcc gcgcaccgcc gccgcggccg cccgtgtatt actcgcctat
     1861 ctgctgctct ggttgttgat cttccgggtg cgcgacatcg tgctcgcgcc cgctacccaa
     1921 gagccttggt ctgggtgggg cgagaacgcg gtgatcgtcg cggctgcgtg gattctgtat
     1981 gcatggttcg ccgcggattg ggatagacgg catctcggtt ttgcgaccgg tgagaggggt
     2041 ctgcacatcg cgagggtgct ttacggcgcg gctttgatcc cgttcggcac agcgcacttt
     2101 gtctatctga aagagaccgc tgctctcgtg cccgggtatt tgccggcgca cgacgcttgg
     2161 gcgtacttca ccggcagcgc ctacctcgcg gcgggcgtgg cgctcatcag cggtgtcttc
     2221 gcgcggctgg cagccgtgct agccgctgtg cagatcggca tgttcaccct gttggtgtgg
     2281 ctgccgatag tcgcggcagg ctctaaggac gccttccgat ggagcgagac cctcatctcc
     2341 gtggcgctga ctgccggcgc ctgggtgggc ggattcctat cgcggcatgc cgtttttgca
     2401 gcgcagacct gtgactggtc cttaaacgag ggccttcgag aacagggcca gctggcggct
     2461 ccatgccttt tctgcctggt cgtggttgta cacggcagag tccggtgggc accagccgtg
     2521 ttgagcaccc gcatagacct ccacttcggc tgtcaaatgt gccttcgcga aggcatcctt
     2581 gagcacggtc ttcgcttcag gctgacgcgc gtcgtcgttc tccgcgatgg ccaccaggta
     2641 tcgcgccttc atctgcggga tgagcagatg cgggctatcc ggcttgtcgg tcacgagact
     2701 gccgccgtga aacgtcgctg ccgcccccac gcgatccgga taggcggcgg cggtgcgaaa
     2761 cacaaagggg ccgcccatgc agtagccggt cgtgcccatt ttcttcttct tgtcgactgc
     2821 cggctgtgca tcgagaaagg ccacgaatgc ctttgcgtca gtcgccgccg tggcggcgct
     2881 caacgttccc gccatggata ggagggcttg ccgcgttgca gggtcgttga aatccggatg
     2941 ttccggcgcg gtcggtgcct tcttgattcg atagaacgga ttgatgacga gaaccgcata
     3001 gcccgaactc gccagccgcg tcgccatctc cttggacgca ggccgcaagc caaaaatatc
     3061 cggccacgtc agcaccgcgg gatattttcc cgtgaccgga ctgacgaagt aggcatcgca
     3121 tgtgccgtcg gcagttttaa tctcgacgtc ggctccctgg gtctgcgccg cgtccgcgag
     3181 tctcggg
//
LOCUS       DASWZT010000345         8647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_65581, whole genome shotgun sequence.
ACCESSION   DASWZT010000345 DASWZT010000000
VERSION     DASWZT010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8647
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65581"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VGE92_09490"
     CDS             <1..351
                     /locus_tag="VGE92_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314101.1"
                     /translation="RLLNALKTRADADKKGLTQLDAEATKNPAGELDVKLGEVYYGVG
                     DYQNAITAITRGLQKGQIKHQDEAYVYLGRSQVALKNTPDAKRAFGGLKTVPNISPRV
                     LKLWDLYSDTLGKS"
     gene            370..852
                     /locus_tag="VGE92_09495"
     CDS             370..852
                     /locus_tag="VGE92_09495"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY1314102.1"
                     /translation="MTIKAGDKMPEGSFTRMTGDGPQKITTEQLFGGKTVVLFSVPGA
                     FTPTCDAKHLPGFVELADEIKAKGVDTIACTAVNDVFVMNAWGKHGGVGDKILMLADG
                     NGTYAKALGLELDGSKFGMGTRGQRFAIIVKNGVATQVNVEGPGEFKVSAADYVLKQL
                     "
     gene            865..3195
                     /locus_tag="VGE92_09500"
     CDS             865..3195
                     /locus_tag="VGE92_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY1314103.1"
                     /translation="MLALRTSPPSFGIAEGERIARDGYGVAASVSALAGERDCNFRLR
                     TADEPRAAAREFVLKIIELAIDDGGADCLASVLGHLAEQDPTLPVPRIFPTLAGESIG
                     RIQRDGREFATLLLSFLPGRLMTEAAPSAGLLHDMGAMLARVDRALQGFFHASLLRRL
                     AWDVRRLPELAQFSHYLDSSALKARVDQISSAFRDCLPRLRGLRSQAIHGDCHAANLL
                     VDSAARAICGVLDFGDMIHAPRIFEPAVAISELLTEAVAPLAAGAALLRGYAEGQASL
                     QADEVESLFDIVGARHCVTLLVHAWRREHDPPGAALLTEAAQHGARSVEQLLGADRQA
                     LIRSWHEAAGTSRPAARIPAGSVDLKRRRRLLGAGAELFYEQPLHLVRGAGVWLYDPQ
                     GRAYLDVYNNVPHVGHTHPAVVAAIQQQTALLATHTRYLHANILDYAQALTARMPRHL
                     DACIFVNSGSEANDVAWRMARMATGHEGALVMEHAYHGITEACAALTVGGGRPSDPRV
                     VTLAPPPSGLRVSDSMSALQLAAAADDVDRAIARLRERGLAPAAFFIDTAITSSGIFD
                     PPPAWAETLSARLRAAGGLIVADEVQYGLGRCGSHFWGFERRGLEPDIVTLGKPIGNG
                     YPMGVVVANCSLIEAFQSRFGFFSTFGGNAVAAAAGLAVLKVLEEERLMANACNVGGY
                     LREQLKSVAARHSCLGEVYGAGLLLGLQVLGRDTAAAKRRNKGIINTLAAQFRVLIGY
                     EGPHADILKLRPPMPFGREHADALVAAIDAAAAARD"
     gene            complement(3192..4136)
                     /locus_tag="VGE92_09505"
     CDS             complement(3192..4136)
                     /locus_tag="VGE92_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEY1314104.1"
                     /translation="MNKVLIVGEHDGKSLNPATAKCVTCAGTIPEAEITIALLGKDTA
                     AVAAQAAAVSGVKTVLRIDRAENEHPLAAIFAPQLAALAADYTHVFGPSTSFGKDLMP
                     RVAALLGVPQVSDLMAVEAAFRFRRPIYAGNAVLTVEADPARKLVATVRVASFEAAKA
                     GGSAAVESKALTAALPSHTRYVGARTGSTDRPDLQTAKRVVSGGRALGSAENFKLLFS
                     LADKIGAAVGASRAAVDAGYAANEMQVGQTGKIISPDIYIAIGISGAIQHLTGIKDAR
                     TIVAINKDGEAPIFEVADVGLVGDLFQVVPELERLVSG"
     gene            complement(4133..4885)
                     /locus_tag="VGE92_09510"
     CDS             complement(4133..4885)
                     /locus_tag="VGE92_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEY1314105.1"
                     /translation="MQKIMVCIKRVVDYNVRIRVKPDGSGVMLDGVKMSVNPFDEIAL
                     EEALRIKERGANIEVVAVSIGVADTQQQLRTALAMGADRAILVQTDVAVEPLTAAQTF
                     LALAKKENPDLIFLGKQAIDDDANQTGQMLAAIWERPQATYASKVEISGSKAAVTREV
                     DAGLETIEVDLPAVITVDLRLNEPRYVKLPDIMKAKKKPLEVTSLEALGVSAGVLLKT
                     VKTEPPAQRQKGVMVKDVAELVDVLKKKGLIQ"
     gene            complement(4897..5709)
                     /locus_tag="VGE92_09515"
     CDS             complement(4897..5709)
                     /locus_tag="VGE92_09515"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY1314106.1"
                     /translation="MESTRPIRALMRGLDALTVLNLRDGATVSEVAQEIRLPRTTVYR
                     ILETLCNAGFVFRDAADDRYRLTILVRGLSDGFDDEAWVTQIAKPLIHELCRETVWPI
                     SIATLSGTTMLVRESTDHLSPLAIERYCAGLRAPLLTTAAGRVYLAHSAAEQRDTLID
                     ILARSDKEDDKLARSTVELKRMLAEIRAAGFAATARTRRLVEEISLSVPVLLHERVLA
                     GLTVRFAAAAVPLKSGIERFLPRLRHCAARISTQFLEQQAEARSKGASQTAA"
     gene            5805..6731
                     /locus_tag="VGE92_09520"
     CDS             5805..6731
                     /locus_tag="VGE92_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY1314107.1"
                     /translation="MLRYFLTRLAGAVPTLFVIVSIAFFLIRAAPGGPFDQEQSLPPQ
                     VMANLQNAYGLDQPILIQYGRYLRAAAHGDFGPSFKYKDFSVTELIGQGFPVSLELGS
                     IALALALLLGVPIGTFAALHRNRAADYAATSLAVVGIALPSFVVLPFLGLLFGIYLHW
                     LPVAGWEPGSVRHLALPVLALMLPPLSVIARLVRASMIEVLRTHFIRTAVAKGLPLRT
                     VIWRHALRPALLPVVGFIAPAVASIMTGSLVVESIAGLPGIGRYMVQGALNRDYTLVM
                     GMVIIYSALLIVMGLVADLLYVWLDPRVRLGR"
     gene            6728..7573
                     /locus_tag="VGE92_09525"
     CDS             6728..7573
                     /locus_tag="VGE92_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY1314108.1"
                     /translation="MRSFLDDASRRLRANRGAMLAMTVIGTMILLVVVGPALNPNGGE
                     TLDWLHVASRPAAQGAHWFGTDRLGRDLYVRTLEGARVSLAVGLVASAVSLLVGIGYG
                     AVAGYVGGRTDHTMMRIIEILSGLPLIFFVIFLTVIFGRNEYLLFLSIGAVGWLTMAR
                     IVRGQTLSIRQREYIEAAVASGASTARIIVNHIIPNLIGPVIVYATLTVPQIIMFESF
                     LSFLGLGIQEPHASLGTLIAEGAGEMEAAPWMLLIPGGFLAVLLLSLNIFGDGLRDAV
                     DPEGS"
     gene            7576..8544
                     /locus_tag="VGE92_09530"
     CDS             7576..8544
                     /locus_tag="VGE92_09530"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1314109.1"
                     /translation="MSSLLQVEDLTVSFSRPNGAIDAVRQVSLCVAAGECLGIVGESG
                     SGKTQIFMAALGLLANNAAAAGSVRFDGEEILAAQPALLNRVRGSKLSMVFQDPMTSL
                     TPHLRIGTQLAEVLVCHAGVSWSEARSAALRMLTRVRVPEPQRRLNQYPHELSGGLRQ
                     RVMIGMSLLCAPALVVADEPTTALDMTVQAQIIELLRALRREIGMALVLISHDLGVVA
                     GLADRIIVMYAGRIVEMAGAVDLFEHAQHPYTAELLKCIPSLSGPRLQRLPTLLGQPP
                     RPGEIGAGCAFAPRCPRAAERCRAERPRLEGSDAARRVACHFPLPS"
BASE COUNT         1463 a         2705 c         2758 g         1721 t
ORIGIN      
        1 cgcttgttga acgcgctaaa gacgcgcgcc gatgcggaca aaaaaggctt gacgcagttg
       61 gatgccgagg ccaccaagaa tcctgcgggc gagctcgacg tcaagttggg cgaggtttat
      121 tacggcgtcg gcgattatca aaacgccatc acggccatca ctcgcggcct gcaaaaaggt
      181 caaatcaagc atcaggacga ggcctatgtg tatctcggcc gatcgcaagt ggctctcaag
      241 aacaccccgg atgccaagcg ggcattcggt ggcctaaaaa ccgttccgaa catcagccca
      301 cgggtcctca agctgtggga cctgtattcc gacacgctgg gcaagtcgta gtacacgagg
      361 agaatcgcga tgacgatcaa agccggcgac aaaatgcccg agggcagctt tacccgcatg
      421 accggggacg gaccccagaa aatcaccacc gagcaattgt ttggaggcaa gacggtggtc
      481 ttgttctcgg tgcctggcgc cttcactccc acctgtgatg ccaagcactt acccggtttt
      541 gttgaactag cagatgagat aaaagccaag ggtgttgaca ccatcgcgtg taccgcagtc
      601 aacgatgtat tcgtgatgaa tgcgtggggg aagcacggcg gcgtcggtga caagattctg
      661 atgctggcgg acggcaacgg cacctatgcc aaggccttgg ggctggaact cgatggatcg
      721 aaattcggta tggggacccg cggccagcgc ttcgccatta tcgtcaagaa cggagtcgcc
      781 actcaggtca atgtggaggg gccgggcgag ttcaaggtct ccgcagccga ttacgttctc
      841 aaacaactct agcgcgaatt tagtttgctc gctctgcgga cgtcgccgcc gtcgtttggc
      901 attgcggaag gcgaacgcat cgcccgtgat gggtacgggg tcgccgcctc ggtcagtgcg
      961 ctagccggcg agcgggattg taactttcgt ctacgaaccg ctgacgagcc acgcgcagcg
     1021 gcgcgcgagt tcgtgctgaa aattatcgag ctggcgatcg acgacggcgg cgccgactgc
     1081 ttggcgagcg tgctcgggca tctcgcagaa caggatccca ctctccccgt accccgcatc
     1141 ttcccgacgc tcgcgggcga gtccatcggg cgtatccagc gcgacggccg cgaattcgcc
     1201 acgttgctgc tcagtttctt acccgggcgc ttgatgaccg aagcggcgcc gagcgccgga
     1261 ctgttgcatg acatgggtgc catgctggcg cgcgttgatc gggcattaca gggatttttt
     1321 cacgcatccc tcttaaggcg cctggcatgg gacgtgaggc gcctgcccga actggcgcag
     1381 ttcagccact acctcgactc atcggcgctc aaagcgcggg tggatcagat ctcgagcgcg
     1441 tttcgcgact gcctaccgag gcttcgcggc ctgcgcagcc aggccatcca tggcgattgc
     1501 cacgccgcca acttgttggt ggattccgca gcccgcgcga tctgcggcgt cctggatttc
     1561 ggcgacatga ttcacgcgcc gcgcattttc gagccggcgg tcgcgatctc ggaattgttg
     1621 accgaggccg tggcgccgct cgccgccggc gcggcgctgc tgcggggcta cgccgagggt
     1681 caggcgtcgc tgcaagccga tgaggtggag tcgctgttcg acatcgtggg tgcgcgtcac
     1741 tgcgtaaccc tgttggtgca tgcctggcgt cgcgagcacg accccccagg cgcggcgctg
     1801 ttgaccgaag cggcgcagca tggcgcgcgt tcagtcgaac agctgctcgg cgccgaccgt
     1861 caggcgctga tccgcagctg gcacgaagcc gctggaacgt cgcgtcccgc cgcccggatc
     1921 cccgccggtt ccgtcgatct taagcggcgg cgccgcctgc tgggggcagg cgccgaactc
     1981 ttctacgagc agccgctgca cttggtacgc ggtgcgggcg tgtggttata cgacccgcag
     2041 ggtcgcgcct acctcgatgt ctacaacaat gttccgcacg tgggtcacac gcatccggcg
     2101 gtggttgcgg cgattcaaca acagaccgca ctgctcgcca cgcacacgcg ctatctgcac
     2161 gcgaacatcc tcgattacgc gcaagcgttg acggcgcgaa tgccgcgcca cttggatgcc
     2221 tgtatttttg tcaactcggg cagcgaggca aacgatgtgg cgtggcgcat ggcccgcatg
     2281 gctaccgggc acgaaggcgc gctggtgatg gagcacgcct accatggcat cacggaagcc
     2341 tgcgccgcgc tgacggtggg cggcgggagg ccgtccgatc cgcgggtcgt cacactcgcg
     2401 ccgccgccgt ccggcctgcg ggtgagcgac agcatgagcg cgctgcaact ggccgccgca
     2461 gcggacgacg tcgaccgggc gatcgcacgg ctgagggagc gcggcttggc gcccgccgcg
     2521 ttcttcatcg acactgcgat caccagtagt ggaatttttg atccgccgcc ggcgtgggcc
     2581 gagacgttga gcgccaggct acgtgccgcc ggcgggctga tcgtcgcgga cgaggtgcag
     2641 tacgggttgg gccgctgcgg ttcgcacttc tggggattcg agcggcgcgg cctcgagccc
     2701 gacatcgtga ccctgggcaa acccatcggc aacggctacc ccatgggggt ggtggtggcc
     2761 aattgcagct tgatcgaagc cttccaatcg aggttcggct tcttctcgac gttcggcggc
     2821 aatgccgtgg ccgctgctgc gggtctcgcc gtgctcaagg tactcgagga ggagcgcttg
     2881 atggcgaatg cctgcaacgt gggtggctac ctacgcgagc aactgaagtc cgttgccgcc
     2941 cggcactcgt gcctcggcga ggtctacggc gccggactgc tgctgggttt gcaagtcttg
     3001 ggacgcgata ctgcggcggc aaaacgccgc aacaaaggca tcatcaacac cctggcagcg
     3061 cagttccgcg ttctcatcgg ctacgaaggt ccgcatgccg atatcctcaa gctgcggccg
     3121 ccgatgccct ttgggcgcga acacgccgat gccttggttg cggcgatcga cgcggcggcc
     3181 gcggcgcgcg actagccgct caccaatcgc tcgagttccg gtaccacttg aaataggtcg
     3241 ccgaccaatc ccacgtcggc cacttcaaag atcggcgctt caccgtcctt gttgatcgcg
     3301 acgatggtgc gcgcatcctt gatcccggtc agatgttgga tcgctccgga gataccgatg
     3361 gcgatgtaga tatccggaga tatgatttta ccggtttgac ccacctgcat ctcgttcgcg
     3421 gcgtagcctg cgtcgaccgc agcgcgcgag gcgccgaccg ccgcgccgat cttgtcggcg
     3481 aggctgaaca gcaacttgaa gttctcggcg ctgccgagcg cgcgcccgcc ggagaccacg
     3541 cgcttggccg tctgcaagtc cggccgatcg gtggagccgg tcctggcgcc gacatagcgt
     3601 gtatgcgagg gcagagcggc cgtcagcgcc ttggattcga ctgcggcgct gccgcccgcc
     3661 ttcgcagcct cgaatgaggc gacgcgcacg gttgcgacca gcttgcgggc cggatcggcc
     3721 tccaccgtca ggacggcgtt tcccgcgtag atgggccttc tgaagcgaaa cgctgcttcc
     3781 accgccatca gatcgctcac ctgcggcaca ccgagcaacg ccgcgacgcg cggcatcaga
     3841 tccttgccaa agctcgtcga tggcccaaag acatgcgtgt agtcggccgc cagcgctgcc
     3901 agctgcggcg cgaaaatcgc tgcgagcgga tgttcgttct cggcacggtc gatgcgcagc
     3961 acggtcttta caccggaaac cgctgccgcc tgtgcggcga cggccgccgt atccttgccc
     4021 aggagggcga tggttatttc ggcctcggga atcgtgcccg cgcaagttac acacttggcg
     4081 gtcgcgggat tgagcgattt tccgtcgtgc tcgccgacga tgaggacctt gttcattgga
     4141 tcaaaccctt cttcttgagt acatcgacca attccgcgac atccttgacc atgacgcctt
     4201 tctgacgctg cgccggaggt tcggtcttca cggtcttgag cagcacgccg gcgctgacgc
     4261 cgagcgcctc gagcgaggtc acttcgagcg gcttcttttt tgccttcatg atgtccggca
     4321 acttcacgta gcgcggctcg ttcaaacgca gatccacagt gatcactgcg ggcaggtcca
     4381 cttcgatggt ttccaatccc gcatcgacct cgcgcgtcac cgccgccttg ctgcccgaga
     4441 tctcgacctt ggaggcgtag gttgcctgtg gacgctccca aatcgcggcc agcatctgac
     4501 cggtttggtt ggcgtcatcg tcgatcgctt gcttgcccag aaaaatcagg tccggatttt
     4561 ccttcttcgc aagcgctaga aaagtttgcg cggcggtgag cggttcgacg gcgacgtcgg
     4621 tttgcacgag aatcgcccga tcggcaccca tggcgagtgc tgtgcgcagc tgttgctgcg
     4681 tatcggcgac gcctatgctg accgctacaa cctcgatgtt ggcgccgcgc tccttgatgc
     4741 gcaaagcctc ttcgagagcg atctcgtcga acggattcac gctcattttc acgccatcga
     4801 gcatcacgcc gctgccatcg ggtttgactc ggattcgcac gttgtagtcg acgacgcgct
     4861 tgatgcacac catgatcttc tgcatttaaa gactccttaa gcggccgttt gcgatgcgcc
     4921 tttgctgcgc gcctcggcct gttgttccaa aaattgagtg cttatcctgg ctgcgcagtg
     4981 gcgcaacctc ggcaaaaagc gttcgatgcc gcttttcagc ggcacggcgg cggccgcaaa
     5041 gcgcaccgtc aaaccggcca acacccgctc gtgcagcagc accggcacgc tcaagctgat
     5101 ctcctcgacc aggcggcgcg tgcgcgccgt ggcggcgaag cccgccgccc tgatctccgc
     5161 gagcatgcgc ttgagctcga ctgtcgagcg agcgagtttg tcgtcctcct tgtcggaacg
     5221 cgccaaaatg tcgatcaatg tatcgcgctg ttcagctgca gaatgcgcga gatacacccg
     5281 cccggcagcc gtcgtcagca gcggtgcgcg caaccccgcg caatagcgct cgatcgccaa
     5341 cgggctcaag tggtcggtgg attcgcgcac cagcatcgtc gttccggaca aggtggcgat
     5401 ggaaatcggc cagacggttt cgcggcacaa ctcgtgaatg agcggcttgg cgatttgcgt
     5461 cacccacgcc tcgtcgtcga atccgtcgga caggccgcgt accagaatcg tcagtcgata
     5521 gcggtcatcc gccgcatcgc gaaacacgaa accggcgttg cacaatgtct cgagaattcg
     5581 atacacggtg gtgcgcggca agcgaatctc ttgggccact tcggacaccg ttgcgccatc
     5641 gcgtagattg agaaccgtca gcgcatcgag gccgcgcatc agcgcacgga tggggcgtgt
     5701 ggattccatg aaacctcgaa cctgatcggt caaatgcggc gcgctagttt aaggatcgcc
     5761 gcttcgcttg tccacctcgc ggtcaatcga tagacttaag tcagatgctg cgctactttt
     5821 tgacccgcct ggccggcgcg gttcccacgt tgttcgtcat tgtcagcatt gctttttttc
     5881 tgatccgcgc ggcgccgggc ggacctttcg accaagagca gagcctgccg ccgcaggtaa
     5941 tggccaacct gcagaatgcg tacggactgg atcaaccgat cttaatccag tacggacgct
     6001 acttgcgcgc cgccgcgcac ggcgatttcg gcccgtcatt caaatacaag gacttctcgg
     6061 tcacggagct catcggacag ggattcccgg tgagtcttga acttggatcg atcgccctgg
     6121 cgctggcgct gctgctcgga gttcccatcg ggacctttgc cgcgcttcat cgaaatcgag
     6181 cggccgatta tgccgcgacc tcgctcgccg tcgtcggtat cgcgctccca tcgtttgtcg
     6241 tgctgccgtt tctcggcctg ctgttcggca tctacttgca ttggttgccg gtcgccggct
     6301 gggaacctgg ctccgtccgg cacctggcgt tgccggtact cgcgttgatg ctgccgccgc
     6361 tgagcgtgat tgcgcgtctg gtgcgcgcga gcatgatcga ggtcctgcgc acgcacttta
     6421 ttcgcaccgc ggttgccaag ggcttgccgc tgcgaaccgt gatttggcgt cacgcgttgc
     6481 gtccggcctt gctgccggtg gtcgggttta tcgcccccgc ggtggcctcc atcatgaccg
     6541 gatcactggt cgtcgaatcg attgccggat tgcctggaat cggccgctac atggtgcaag
     6601 gcgcgctcaa tcgcgactac accctggtca tgggcatggt gatcatttat tcggcgctgc
     6661 tgattgtcat gggtcttgtc gcggatctgc tgtacgtctg gcttgatccg cgcgtgagac
     6721 tcgggcgatg agaagtttcc tcgacgacgc ctcgcgccgc ttgcgcgcca atcgcggcgc
     6781 gatgctggcg atgacggtca tcggcaccat gatcctgctt gtcgtggtcg ggccggcgct
     6841 caatccgaac ggcggcgaaa ctctcgattg gctgcatgtg gcgtcgcgac cggcggcgca
     6901 aggcgcgcac tggttcggaa cggatcggct ggggcgcgat ctctatgtgc gcacgctcga
     6961 gggggcacgc gtatcgttgg cggtcggttt ggtggcgagc gccgtgagtt tgttggtcgg
     7021 aatcggctac ggagccgtcg ccggctacgt gggcggccgc accgatcata cgatgatgcg
     7081 tataatcgag atattgagcg gcctgccgtt gatattcttc gtcattttct tgaccgtgat
     7141 tttcgggcgc aacgagtact tgttgttcct gtcgatcggc gccgtcggct ggctgaccat
     7201 ggcgcgcatc gtgcgcggcc aaacactcag cattcgtcag cgcgagtaca tcgaagcggc
     7261 ggtcgcatcg ggagcgagca cggcccgcat catcgtcaat catataatcc ccaatctgat
     7321 cggaccggtg atcgtttacg caacgctcac ggtgccgcaa atcatcatgt tcgagagctt
     7381 cttgagcttc ctcggcctcg gaatccaaga accgcacgcg agcttgggca cgctcatcgc
     7441 cgagggagcg ggcgagatgg aagcggcacc ctggatgctg ctgatcccgg gcggattctt
     7501 ggcggtattg ctgctgagtc tgaatatttt cggcgacgga ttgcgcgacg ctgtcgaccc
     7561 cgaggggagt tagcgatgtc ctccctgttg caggtcgagg atttgaccgt gagcttttcg
     7621 cgtccaaatg gcgccatcga cgccgtacgg caagtatcgc tatgcgtcgc ggcgggcgag
     7681 tgtctcggca tcgtcggcga gtccggttcc ggtaaaaccc aaatcttcat ggccgccctg
     7741 ggactgctcg cgaacaacgc cgcagccgcg ggcagcgtgc gcttcgacgg cgaggaaatc
     7801 ttggcggcgc agccggcgct cttgaatcgc gttcgcggtt ccaagctcag catggtcttc
     7861 caagacccga tgacctcatt gactccgcat ttgaggatcg gcacgcagct ggccgaagtc
     7921 ttggtctgcc acgcaggcgt gtcgtggagc gaggcgcgca gcgccgccct gcgcatgctg
     7981 acgagagtgc gcgtgcccga gccgcagcgc cgcttgaatc aatatccgca tgagctgtcg
     8041 ggcggcttgc gccaacgggt gatgatcggc atgagcttgc tgtgcgcgcc ggcgctcgtc
     8101 gtcgcagacg aaccgaccac cgcactcgat atgaccgtac aggcgcaaat catcgagctg
     8161 ctgcgcgcgt tgcgccggga gatcggcatg gcgctggtgc tcatcagtca tgacttaggc
     8221 gtggttgcgg gtctcgccga tcggatcatc gtcatgtacg ccgggcgcat cgtcgagatg
     8281 gccggtgcag tcgacctgtt cgaacacgca cagcatccgt atacggccga actgctcaag
     8341 tgcatcccga gtttatcggg tccgcgcctg cagcgattgc ccactctcct gggccaaccg
     8401 ccgcgacccg gagagatcgg cgccggatgc gcgtttgcgc cgcgctgtcc gcgcgccgcc
     8461 gagcgctgcc gtgcggagcg gccgcgactc gagggttcgg acgcggcgcg ccgcgtggcg
     8521 tgccattttc cgttgccatc atgagcggcg gtgccgcggt cgatcgcctg ttgagcgtcg
     8581 ccggtctcgc ggttcgatac ggcgagcgcc aggtgctgtg ggatgtgagt tttgatctcc
     8641 tcgcggg
//
LOCUS       DASWZT010000346         4024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_55948, whole genome shotgun sequence.
ACCESSION   DASWZT010000346 DASWZT010000000
VERSION     DASWZT010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55948"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VGE92_09535"
     CDS             complement(<1..227)
                     /locus_tag="VGE92_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314110.1"
                     /translation="MLEALLARDDSRVGFLTQGIFMSAKGWLEFECRSIGYSALGAVL
                     AAAALWAPAADAQNISSAASESSGTLEEIVIT"
     gene            complement(554..1093)
                     /locus_tag="VGE92_09540"
     CDS             complement(554..1093)
                     /locus_tag="VGE92_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314111.1"
                     /translation="MKSTNTRPVALAAAALGAAFAFAAHADSPPVRIVMLNMAVTLDY
                     VGTERSKAAATKVGDIDRLRIVYDAAAVDPTTHRVRLLNLQHFIAGHYSPAAPDPRIM
                     PLDDAWLDLGVTPYRLHFRAAVVHGDPIIIDIDEVSRRLTIHPQQHPQEAIISGPYAI
                     DSTPITGPEARVAGSATRR"
     gene            complement(1080..2276)
                     /locus_tag="VGE92_09545"
     CDS             complement(1080..2276)
                     /locus_tag="VGE92_09545"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY1314112.1"
                     /translation="MSSHSSRTLNAVSSAELQRRWRAVRDAMRARDIDALVVQNSSDW
                     VGGYIRWFSNQPATNGYPSSVVFPAAGGMSFIEQGPFGGLRTANEAEIAATGIARRLT
                     TPSYPSVGYSGGYDADIAVAELEKFGAQRVGLVGPASMYHSFGQRLQSSLADRDVVDA
                     TDLVDAIKAIKSDEERALIRQVAAMQDAVMAKVREFIRPGLKDFEIAAYAQYLGQQLG
                     SEQGIFLCSSAPVGQAASFRPRSMQGRTLQRGDVYSLLIENNGAGGYYTELSRMFVLG
                     RTPPELKDAHRSVIEAQTFALSLLKPGAPAREVFAQHNAYLRQHDMPEERRLSVHGMG
                     YDMVERPLIRSDEDMQIAENMAIVCHPGILNERMFVHNTDLYLIEAQGPSACLHRTPK
                     EIFEIN"
     gene            complement(2295..3254)
                     /locus_tag="VGE92_09550"
     CDS             complement(2295..3254)
                     /locus_tag="VGE92_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEY1314113.1"
                     /translation="MAANPRIIYIGAPEADAAAFESRARADFPGIDLLATNDRTLASQ
                     VIEHAEVLIGHHFQFDDNMLERAAKVRWIQSLTTGTDAILKLAALRADVTVTSTRGMH
                     GPQMSELVFLQMLALLRDFPRLQRNQAQAHWERWPQPLLWGKTVVIVGVGAISEALAP
                     RCQAFGMKVYGVSGANRRPPGFDGMFGRTELNKAAALADFLVLIVPLSPQTENLIDAA
                     VLAAMQPSAYLINVARGGVLDEGALLEALRERRIAGAALDVFREQPLPPDHPLWRAER
                     VIVTPLIGGMSNVYLDQAYPIVRDNLQHYLAGRAAAMRNIVPH"
     gene            complement(3276..>4024)
                     /locus_tag="VGE92_09555"
     CDS             complement(3276..>4024)
                     /locus_tag="VGE92_09555"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEY1314114.1"
                     /translation="PDIFGQFLPQIHRETDAVINLTTGGSPTMSVADRLAAAVRFQPE
                     MCSLNMGSMNFAIFPAAARIKQWKFPWEEGYVRNSDDFIFRNTFRDIAYILETMRDAG
                     TKFEHECYDVGHLYSLAHFIERGLVKPPFFVQMIFGILGGIGAELDNLMFMKRSADRL
                     FGAANFQWSVLAAGRHQMPFVTQAGLMGGNVRVGLEDSIFIERGKLAESNAQQVLKIR
                     RILAEMGHEPATPAEARQMLGLKGRAHVRY"
BASE COUNT          790 a         1200 c         1237 g          797 t
ORIGIN      
        1 gtgatgacga tttcttcgag cgttccactg ctctctgatg cagcgctgga gatgttttgc
       61 gcatcggcgg cgggcgccca gagcgctgcg gcagcgagga ccgcacccaa ggcggaataa
      121 ccgattgatc tacactcaaa ttcgagccag cctttcgcgc tcataaatat cccttgtgtt
      181 aggaaaccaa ctctcgaatc gtcccgggcg agcagtgctt cgagcacggc aaaccgagcc
      241 tctttgtgtt gcctgcaacg tactcctggg acgtcgcgca tggaaatcgc ttctgccaat
      301 cgggtgcatc acggaacgca atggtgcgaa cgctcggcgc cgcattcgta gttaaaaagc
      361 tacaaaattg acccgaagca gccgggtttt tgacaaaaaa cgcgattttg ttgcgctgac
      421 gactgaagca cccccgacaa tttcccggct tctttttgta ccacaatgcg gtctaaattg
      481 tactgataaa cggtatgctt gcggggaaca atagtcaata gtgaacccat cgccgaacgg
      541 cggggcggcg gttctagcgt cgcgtcgcgg atccggccac gcgggcttcc ggaccggtga
      601 tcggcgtcga atcgattgcg taggggcccg agatgatggc ctcttgcggg tgctgttgtg
      661 ggtggatggt gagccggcgc gacacctcgt cgatgtcgat gatgattgga tcaccatgca
      721 ccacggccgc tctgaaatgc agtcggtagg gcgtgactcc gagatccagc caggcatcgt
      781 cgaggggcat gatgcgcggg tccggcgcag ccggagagta gtgtccggcg atgaaatgct
      841 ggagattcaa cagccgtacc cgatgcgtgg tcggatcgac cgcggcggcg tcatagacga
      901 tgcgcaaccg gtcgatgtcg ccgactttgg tcgctgcggc tttgctgcgt tcggtgccca
      961 catagtcgag cgtcaccgcc atattcaaca tgacgatgcg caccggcggc gagtccgcgt
     1021 gcgccgcgaa agcgaatgca gcgcccaagg cggcggcggc caatgcgacg ggtcgggtgt
     1081 tagttgattt caaatatttc cttcggcgtt cggtgcaagc aggcggaagg tccttgcgct
     1141 tcgatcaggt agaggtcggt attatggacg aacatccgtt cgttcagaat ccctggatga
     1201 cagacgatgg ccatgttctc ggcgatctgc atatcctcgt cactgcgaat gagcggccgc
     1261 tccaccatgt cgtagcccat cccgtgtacg ctcaagcggc gctcttccgg catgtcatgt
     1321 tgccgcaggt aggcattgtg ctgcgcgaac acctcgcgcg cgggcgcgcc gggtttcagc
     1381 agcgagagag cgaaggtctg cgcttcgatc accgagcggt gtgcatcctt gagttcgggc
     1441 ggcgtgcggc cgagaacgaa catccgcgac agttcggtgt agtagccgcc cgcaccgtta
     1501 ttttcgatca acagcgaata aacgtcgccg cgttgcaatg ttcggccttg catggagcgg
     1561 ggcctgaagc ttgctgcctg ccccacgggc gccgacgaac aaagaaaaat cccttgttcg
     1621 ctgccaagtt gctgcccgag atattgagcg tacgcggcga tctcgaaatc cttgagcccg
     1681 gggcggatga actcgcgcac cttcgccatg actgcatcct gcatggccgc cacctggcgg
     1741 atcaatgcgc gctcttcgtc gctcttgatt gccttgatgg catcgacgag atcggtcgca
     1801 tcgacgacat ccctatccgc gagcgatgac tgcaaacgtt ggccgaagct gtgatacatc
     1861 gaggcgggac ccacgagtcc aacacgttgc gccccgaact tctcgagttc ggcaaccgcg
     1921 atatccgcat cgtaaccacc cgagtagccg acggagggat agctcggcgt ggtcagtcgt
     1981 cgcgcgatgc ccgtggctgc gatctcggct tcattggcgg tgcgcaagcc gccgaacggg
     2041 ccctgttcga tgaacgacat gccgcctgcg gcggggaata ccacgctgct cggataaccg
     2101 ttggtggccg gctggttgct gaaccagcga atgtacccgc ccacccaatc gctggaattc
     2161 tgcacgacca gagcgtcgat gtcgcgggca cgcatggcgt cgcgtactgc gcgccagcgg
     2221 cgctgtaatt cggcggagga gaccgcattg agagtgcgtg aagagtggct ggacatggct
     2281 tgctgctgtt gtgactaatg aggaacgata ttgcgcatcg cggcggcacg gccggcgaga
     2341 taatgttgca gattgtcgcg cacgatggga taggcctgat cgagatacac attactcatg
     2401 ccgccgatca agggcgttac aatcacgcgt tctgcgcgcc ataagggatg atccggcggc
     2461 agcggctgtt cgcggaagac gtcgagcgct gcgcccgcga tacgccgttc gcgcaaggcc
     2521 tcgagaagtg cgccctcgtc gagcacacca ccgcgcgcaa cattgatcaa atacgcgctc
     2581 ggttgcatcg cggccagcac cgcggcgtcg atcaaattct ccgtctgcgg cgacaacggc
     2641 acaatcaaca ccaggaaatc cgccagcgca gccgccttgt tcaattcagt gcggccgaac
     2701 attccatcga accccggcgg ccggcggttg gcgccggaaa cgccatacac cttcatgccg
     2761 aacgcctggc accgcggcgc caaggcctcg gagattgcgc cgacgccgac gatgacgacc
     2821 gtctttcccc agaggagcgg ttgcggccaa cgttcccagt gcgcttgcgc ttgattgcgc
     2881 tgcagccgcg ggaagtcgcg caacagcgcc agcatttgca gaaatacgag ttcggacatc
     2941 tgcgggccgt gcatgccgcg agtcgaggtc accgttacat ccgcccgcaa cgccgccagc
     3001 ttgagaattg catcggtacc cgtggtgaga gattgaatcc agcgaacctt ggcggcgcgc
     3061 tcgagcatgt tgtcgtcaaa ttgaaagtga tggccaatca acacctcggc gtgctcgatc
     3121 acttgcgagg ccaaggttcg atcattggtg gccaacaggt cgatgcctgg gaaatctgcg
     3181 cgcgcgcggc tctcgaacgc cgccgcgtct gcttccggcg cgccgatgta gatgatgcgg
     3241 gggttcgctg ccatttcgtg gaacttgatt gccgatcagt acctgacgtg cgcccggccc
     3301 ttgagcccga gcatctggcg ggcttcggcc ggcgtcgccg gttcgtggcc catctccgcc
     3361 aggatccgcc ggatcttcag tacctgctgc gcattcgatt cggccaactt gccgcgttcg
     3421 atgaagatac tatcttccaa gccgacgcgc acattacccc ccatcagccc cgcctgcgtg
     3481 acgaacggca tttgatggcg cccggcggca agcaccgacc actgaaaatt cgccgctcca
     3541 aacagccgat ccgcgctgcg tttcatgaac atgagattgt cgagctccgc gccgattccg
     3601 ccgagaatgc cgaatatcat ttgcacgaag aacggcggct tgaccagccc gcgttcgatg
     3661 aaatgcgcca ggctgtataa gtgtccgacg tcatagcact catgttcgaa cttggtgccg
     3721 gcatccctca tggtttccag aatgtaggcg atgtcgcgaa acgtattgcg gaagatgaag
     3781 tcatcgctgt tgcgcacata gccttcttcc caggggaatt tccactgctt gatgcgagcc
     3841 gcggcgggga atattgcgaa attcatcgat cccatgttga gcgaacacat ctccggctgg
     3901 aaacgcacgg cggcggccag gcggtcggcc accgacatgg tagggctgcc gcccgtggtc
     3961 agattgatga ccgcgtcggt ttcgcgatga atttgcggca ggaattggcc gaagatgtcg
     4021 ggac
//
LOCUS       DASWZT010000347        21183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_89723, whole genome shotgun sequence.
ACCESSION   DASWZT010000347 DASWZT010000000
VERSION     DASWZT010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 21183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21183
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89723"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VGE92_09560"
     CDS             <1..443
                     /locus_tag="VGE92_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314115.1"
                     /translation="GREAAARLPPGIVVAGKTGTSSDTRDSWFAGFTGSYLAVVWVGY
                     DDNRVTGLTGAAGALPVWADTMAALSSTSFQPVPPELIEERWIGFADGLETTPACSAD
                     AVAVAVPTGTVLPANTNCGPRPAPGSPSGTMGDKIKTWLKHIVH"
     gene            419..913
                     /locus_tag="VGE92_09565"
     CDS             419..913
                     /locus_tag="VGE92_09565"
                     /inference="COORDINATES: protein motif:HMM:NF024820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1314116.1"
                     /translation="MAQAHRSLTLAACLAAAVLLSGCPAPEPRPMPPPGTSFPESPPS
                     VPAQPAPAPPRENHLSAATHSLVTQARTLETHGDLDGASSTLDRALRIEPNNPLLWIE
                     IGRLRLAENDAHQAEGCGRKALVLASGDRRTQAQAGHLLADALRAEQRNQEASEVEHQ
                     PFMQ"
     gene            921..1523
                     /locus_tag="VGE92_09570"
     CDS             921..1523
                     /locus_tag="VGE92_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314117.1"
                     /translation="MRVARRLLWGSLSGTALLSAALIAFNTFGLAARLCTIPGAQPGV
                     SDACGRLGLGGRPIRSERLAWESRTPGSCQALREHISRFPGGAYRSEAADLLTARRTS
                     TIETWEPASRTLALFEPAGSAAKDEVIAQAHALESAKADAERLCRGFGAGSLYRFVSA
                     SAVAERWSCTRGPQGTVCGFDGHAQCELSVSRRTEREQCG"
     gene            1871..3808
                     /locus_tag="VGE92_09575"
     CDS             1871..3808
                     /locus_tag="VGE92_09575"
                     /inference="COORDINATES: protein motif:HMM:NF011999.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HEY1314118.1"
                     /translation="MIFDDFKTSYEKSAAANFTLAEYFELCRNEPLTYGLAAERMIKA
                     IGEPRLVQTKSDPRLLRLFGNRTLKYYDAFSDFYGMEGGIIEEIVSFYRHAAQGLEER
                     KQVLYLLGPVGSAKSTLAIKLKSLMERYPIYTLVDGDTGERSPLLESPLGIFDPEKFG
                     ARLEGEYGISPRYLTGISSPWVQKRMRAYNGDLTRFKVQRLYPSVLNQLAVAKTEPGD
                     ENTQDISSLVGKVDMRKLSKLGQDDPDAYSYSGGLCLTTQGLLEFVEMFKAPIKVLHP
                     LLTATQEGNFKGTEGFGAMPYQGTVLAHSNESEWNKFRSNRDNEAFLDRIFIVKVPYC
                     LRVTEEVLIYQKLLRESTLSNAPCAPGTLPMLARYMVLSRLKAPSNSSIYSKLRVYDG
                     ENLRESDPQAKSVAEYREMGGSDEGMTGFSTRYAFKLISKAFNRDASEESADPVTLLK
                     VIEESIGTSDFSPDLQKQCTVHLKDFLIPRYLEELTKEIQAAYLESYADYGQELFDRY
                     VAFADFWIQEEVYVDPATGVAFDKAALNSELEKMEKPAGIGNPKDFRHEIVNHVLRAQ
                     NKSGGDKLVWTSYQKLREVIEKRMFAATDDLLPVVSYFAKGNAEDEKKHEAFVARMKA
                     RGHTPTQVRRQVEWWTMARRQ"
     gene            3837..5111
                     /locus_tag="VGE92_09580"
     CDS             3837..5111
                     /locus_tag="VGE92_09580"
                     /inference="COORDINATES: protein motif:HMM:NF003707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HEY1314119.1"
                     /translation="MTSVIVDRRPDKGKSVANRQRVLKRLDGALKAQVDKMIARRKLQ
                     DHDKGVEVTIERKDVKEPSFTLDGATGSSGRVIPGNDRYRVGDQIPRDPAQARGRGAG
                     SGTGEGSSDDEDVFRFALSREEYLSLLFDELELPALVKKDLLEIDENRFRRGGVVRYG
                     SPGTVCVARTFKASIGRRVAAEAQHEEALDAAEAELLAARASGVQAQVQAAEERLQEV
                     RQGGAAIPFLDPVDLRHRNLVEVQAPRSAAVMFCLMDVSSSMDESRKDLAKRFFTLLY
                     LFLSRKYQKVELVFIRHTDQAQEVDEDTFFNGTQAGSTRVLSALEKMHEIIGLRYPAS
                     HYNIFGAQASDGDSFGADSSESSAYLKSQLLPSSRYFVYAEVGEKSAPGTTRLWAAYD
                     DIECDHFNMATVGARDEVYPALAKLFQKEQPA"
     gene            5111..6580
                     /locus_tag="VGE92_09585"
     CDS             5111..6580
                     /locus_tag="VGE92_09585"
                     /inference="COORDINATES: protein motif:HMM:NF016201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEY1314120.1"
                     /translation="MKEQRMLTGSEWTLDDLLGIEQICAAHAERYGLDTFPNQLEVIT
                     SEQMLDSYVSSGLPVNYGHWSFGKHYLIESGKYRSGANALAYEIVINTNPCIAFLMEG
                     NSLAMQALVIPHACFGHNSFSKGNYLFKQWTQPDAVLDYMLFARNYIADCEEHFGLDR
                     VERTLDALHALRDQGLDKYKRPRKLDTRKERAEQAKRLSVAQETMRQAEYYSVVPHAA
                     KAESAAAVFPPSPEENLLYFIEKHAPKLEPWQRELARIVRKIAQYFYPQRQTQVMNEG
                     WATFWHHRLINDMYDARQIDQGILLECLHSHTNVIRQHEYSRLNPYALGFAAWHEIKR
                     VCEAPTAEDREYLPDVAGKDWLETFHDIMQNYRDESFLLQFLTPKLVREFRMMNIQTA
                     EGLEHWNVENTAGEEGFQAIRSQLAANYRLESMMPEISVVRYGRDTDRKLLLQHQVYN
                     GRTLDESETEQTLKHLRALWGFEVALEAVDQNGAVLKTY"
     gene            6589..8406
                     /locus_tag="VGE92_09590"
     CDS             6589..8406
                     /locus_tag="VGE92_09590"
                     /inference="COORDINATES: protein motif:HMM:NF014822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY1314121.1"
                     /translation="MPASLRPSMGATPYADSSGSGVTFRVWAPFASALNVAGDFNAWS
                     PSATPLYAEGNGYWSVDTPAAALGSQYKFVVTGGGATLWRVDPYARCISRSNGVLNCR
                     VAAADESYSTPGYSTPAWDQLVIYEMHVRTFLYCPDGIDGKGSFRSAISKLPYLRELG
                     INAIELLPIGEFIGDISAGYNPAYIFAIEDEYGGPDGFRQFVNAAHQQGIAVIMDVVY
                     NHLGVPAADMWVFDGWSQNGKGGIYFYNDWRSSTVWGETRFDYGRPEVRAFLRDNALR
                     WLESRFADGLRWDSTGSIRNVYDANDDTANDIPEGWSLMQWINGLVAQSQPWKICIAE
                     DMHENEWITKDPGSGGAGFGAQWSSAFVSTVRAALVGSDDGRRDMYAVAAVIASRYNG
                     NAFQRVVFTESHDADSNGDQRVPEMIWPGNAQSWYSKKRSTLGAALVMTVPGIPMLFM
                     GQEFLSGGWFNPDAPLDWSNAEAFPGIILLYRDLIRLRRNWYDKTRGLSGQHVNVHHV
                     NQSDKLIAFHRWEQGGSGDDVVVVLNFSTQAFGIYTIGVPREGTWRVRLNSDWSGYDG
                     SFGNAPSVDAAARGIAYDSMPASASLAIGPYSAVILSQD"
     gene            8546..9955
                     /locus_tag="VGE92_09595"
     CDS             8546..9955
                     /locus_tag="VGE92_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY1314122.1"
                     /translation="MPLTPDRMATMSRLLDAALPLDEAGRRRWLEALAPEYHDLLPEL
                     REALLAEFYTALPPATGAERGAAAATGLAPGAHVGPYELIRLLGAGGMAEVWLAKRAD
                     GVFKREVALKLPLLTRVRQHFEQRFARERDILAGLEHPHIARLYDAGIDPDGLPYLSM
                     EYVQGRLLTEWCDGECLDIRQRLQLFLQVLEAVQYAHDRHVIHLDIKPSNILVSDTGQ
                     SRLLDFGVAKLLEDDAATGQTPLTKVYGHVITAVYASPEVVRGDPVDARSDIYSLGVV
                     LFELLTGVQIAAFGRIRQAITAMGKAVELDPLNAYAWGNMGLFLTMDRDYPAARRALN
                     RALAISPDSDALHFALGELDMLEGEFAHASAEFAKEGNEGARRMSDALLEHARGNETE
                     SQHALDDLIAAHAADMAYQVGDVYAWRGDKDEAFEWLERAYRQRDSGLNGIVYDPLLA
                     GLKDDPRYGKLLKELELSN"
     gene            complement(9968..10981)
                     /locus_tag="VGE92_09600"
     CDS             complement(9968..10981)
                     /locus_tag="VGE92_09600"
                     /inference="COORDINATES: protein motif:HMM:NF018828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314123.1"
                     /translation="MFVVHSYALAVVFCVLTMFCWGSWATTKKLARPNWGFALFYWDY
                     TLGVLLLTLIFGLTLGSTGGEGRAFLADLSQASGASLGSALLGGAIFNLANILLVAAI
                     EIAGMAVAFPVGIGLALILGVIVNYLAAPVGNVTLLATGVLLVTLAIILDAAAYRRLP
                     GATPDAGRKRSNTGLVLSIACGVFMGMFYRFVAAAMYADPAHATAGMMGSYAAVFVFA
                     VGIFLSSFLWNTIAMKKPFVGAPVSFRDYFAGSMGTHMTGILGGVIWGVGMSLNLIAS
                     GRAGFAISYGLGQGATMVAAFWGVFIWKEFRTAPPGTPTLLSAMFASFLTGLALIVLA
                     RTL"
     gene            complement(10986..11924)
                     /gene="rbsK"
                     /locus_tag="VGE92_09605"
     CDS             complement(10986..11924)
                     /gene="rbsK"
                     /locus_tag="VGE92_09605"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEY1314124.1"
                     /translation="MPNSPRIAVVGSANTDLTTFTDVFPRPGETLFGKSFDLGFGGKG
                     ANQAIAARLCGADVLMVAKVGRDLFGQATIDNFAAFGVDTRHVGIVEDASTGVAPIFV
                     EPNGQNRIIVVKGANDCLRPADVDAAAAELKRVDAIILQFEVPLDTVYHTIRFARAHH
                     IRCIVNPAPAVPANLAELAGADYFIPNETEAEKITGLAAGTEAEAAAAAARLLSLGFQ
                     RIVVTLGARGSLLAGAEGHTLVAPFAVKATDTTGAGDAFIGSFAVFLAEGVREPEALA
                     RANLYAALSTTRVGTQKSFPRRVDFDAEWARRRAKD"
     gene            complement(12150..12899)
                     /gene="deoC"
                     /locus_tag="VGE92_09610"
     CDS             complement(12150..12899)
                     /gene="deoC"
                     /locus_tag="VGE92_09610"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEY1314125.1"
                     /translation="MTRTQSVRRLLSLTDLTSLNDADDAAAVHALSKLARSAPVKPAA
                     VCIWARLIPAALESLRGSGIAVCAVANFPGGAADPAGAARETSAAVDAGAAEVDVVFP
                     YRALLAGDAGVGLMLVRACRQACAGRALLKVILETGQLGGADTIRHAAEIAVEGGAHF
                     LKTSTGKTQPAATAAAAAVLWDVIAAAAGRGQAIGFKASGGIRTFDDALIYLGGYDAR
                     FGEGSATAANFRIGASALFKELLAALAASGE"
     gene            13036..14097
                     /locus_tag="VGE92_09615"
     CDS             13036..14097
                     /locus_tag="VGE92_09615"
                     /inference="COORDINATES: protein motif:HMM:NF018249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside permease"
                     /protein_id="HEY1314126.1"
                     /translation="MPLKTLAAALLCVVAAVARAAAEHTPIEVKVVVVTMFEIGADSG
                     DAAGEFQLWYERQKLNRRYPFAHYHDLFLNRHSGVLGMVTGEGSINSASAVMELGMDP
                     RFDLSHAYWLVAGIAGVDPDAASIGSAAWAEYLVDGDLAYEIDAREIPADWPTGYFAR
                     NTQKPYDPEKPAPSGEVFRLNPDLVQWAFELTRAIKLDDGKELQAARAQYTGYPNAQK
                     PPFVLKGDTLSAMTFWHGKILNEWAHRWVDYWTDGKGRFVTSAMEDTGTLSSLTYLHG
                     TGKVNKDRVMVLRTASNYTVQPPGVSAADHLLKENAGYAGLHASVEAAYRVGSAVVDE
                     IVGKWSVYREHAPQPGAPR"
     gene            complement(14105..15538)
                     /locus_tag="VGE92_09620"
     CDS             complement(14105..15538)
                     /locus_tag="VGE92_09620"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314127.1"
                     /translation="MRAALGSLLAGLCCLALNTRSHAAAPWFDAVKASATPEQLYRFL
                     YALPKGGDLHNHLAGAVRSEWFWDAAMAAQQRGYTYYTRVRIENCAPYGHDEFGASKA
                     LLLFKIIQASTFDRLDACGKSEFKRLQDLDAREKEAWLNSMRLDAPYEGRNEFFEAIW
                     ARINDMLRNPYLICDILLRNMQAFGKEGLAYLETQQGLENAVRPDGTPIAVDEVVGIY
                     RACLASPAAKATGVEVRFQNVLLRYAPDAEQHLRTLYAITDRYRDLYVGVNMAGREDN
                     NRGYPLRFLPVLRELRHQYPDMHLSIHAGEADEPNFHVRDTLLLGAERIGHGVNLISD
                     PDTLVRMRYGPFMVEINLISNLLLEYVKDYSQHPFPEYLRTGIPVALSTDDRGMWDSN
                     LSDEFYVAVKEFNLSWEEIVKLGRNSLQYGFVDEATKQRLLADYDKRVADFARSFQRK
                     GWSSLSATRPVSYGFLCRHYQLCLPAE"
     gene            complement(15547..16656)
                     /locus_tag="VGE92_09625"
     CDS             complement(15547..16656)
                     /locus_tag="VGE92_09625"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEY1314128.1"
                     /translation="MILVAALLSTISGTAEAADAGHKRKVIIDQDALGPAGSNMQAIL
                     LLLQSSDVEVLGITITSGDGWRDEEVDHALRLLEIAGRTDVPVVPGAVLPLLNSPART
                     RAWEGAYGKLYYKGAWTEHWPDQGVNRRVPHPDDPYLVPSSPAGTAHIKAATESAADF
                     LIREVRRYPGQVTLIAAGPLTNLALAARLDPKWSGLAQELVFMGGSFNPTAADNAFAA
                     EYANAPRREFNMRFDPEAASMVLHEPWRKVTEVPVDPTTKTLFTAEMQHRVGRGKAPF
                     DAYIEKFGQPYPMWDELAVAVWLDPGIVTQSKTLLVDVDTSFTAGYGDTLSWSPGEGP
                     NLGEQPVQVVFDIDLKKFEALSLDLLTRPAPGHAE"
     gene            16829..17059
                     /locus_tag="VGE92_09630"
     CDS             16829..17059
                     /locus_tag="VGE92_09630"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HEY1314129.1"
                     /translation="MVTKRVSSVKSVPSMKKTVIRSTVASAIDPDVRRKLVAAEAYFL
                     AERRGFTAGNELEDWVAAEAMVDSKLRQLQVA"
     gene            complement(17105..18232)
                     /locus_tag="VGE92_09635"
     CDS             complement(17105..18232)
                     /locus_tag="VGE92_09635"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HEY1314130.1"
                     /translation="MTHAAIVGWGKCLPPSILTNHDLATFLPTDDEWITSRTGMKQRR
                     ISHVPGIELAIVAARRAIACAGMSGADIDLVIYGSCSNDETVPNSASGVQQRIGATQA
                     AAFDVNTACTSFMYGLSIGSSMIKTGIVKNALIIGVELISQYMDWDNRNVAVLFGDGA
                     AAMVLRESEHESGVIAEKLQCYADSRQILRVRGMGTMYANLGVQFGQTRWDFDGQEIF
                     RKAVQGMSEASLDVMRRAGVTMQDIALVVPHQANLRIIDAVAKRAGAGPDKLYLTVQK
                     YGNMSSATAPVALVEAVEEGRVAPGNLVLMPAFGGGLTLSAHLIRWGERVTPLQKCDA
                     ELPPCAKTALEIVNEIRAQKKLGEAEAKRFETLRFPENTPP"
     gene            complement(18313..19800)
                     /locus_tag="VGE92_09640"
     CDS             complement(18313..19800)
                     /locus_tag="VGE92_09640"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY1314131.1"
                     /translation="MMSEVWAKWENRVINGVLPLRRFLGRSNHSVVFLTEYKPQNLAN
                     AAIKLVPADPAAADTQLEYWKRAAALAHPHLIRLFDTGRCQLGGHPFLFVVMEYAEET
                     LAQILPKRALTPDEVREMLRPTLDTLAYLHAKDLVHGQLKPANFLVIDDQLKLSSDNI
                     HPVGRSRPSIAKRSVYDPPEAKIGGIDDKGDVWGLGVTMVEALTQRLPAGLDQRTEIA
                     VLPPAPIPAGFEGVTERCLNRDPALRPTISDLKVQFDPSPCPAEAAKPAAPAEDGAES
                     APVTPVGPVPPIPPIPPVAQSAAASAAQPELSLTELLVRAPNPDALSKFPDRRLPVWG
                     TVAGLVLAGSVWAVWHHQHPGSPVSTAPVPVPAPVAQSAAASAVPQNSAPAPVAASAS
                     AVAPAVDPSRSVRHEEIPHISRGARASIRGDIVVTVRVTVDRSGNVVDEALENRGNSK
                     YFARAASEAARKWRFVAADHPDAREWLLQFEFTRGGTSGHAIPRS"
     gene            complement(19870..20148)
                     /locus_tag="VGE92_09645"
     CDS             complement(19870..20148)
                     /locus_tag="VGE92_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314132.1"
                     /translation="MTAGPLTEVLATGHPWRARAPDGADIFSGLPSGYPPASAYLAIR
                     LDTRARALGWLCVAEKIGANGFDARDERLLSSVGSLASRLYEAGSPQI"
     gene            complement(20145..20321)
                     /locus_tag="VGE92_09650"
     CDS             complement(20145..20321)
                     /locus_tag="VGE92_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314133.1"
                     /translation="MTRKLTETSNALAAANARIAALVDLNIQLAAAQDTEKVLQGMCA
                     AASTSESDRRRRRW"
     gene            complement(20328..20744)
                     /locus_tag="VGE92_09655"
     CDS             complement(20328..20744)
                     /locus_tag="VGE92_09655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1314134.1"
                     /translation="MAKILVVDDSSVYRKELVALLSSDGHLTIEASDGLDGLIVARAE
                     RPDVVISDIMMPTIDGFGLVRALREDPELSATRVIFCTANYNEQETKTLARACQVGFV
                     LMKPCEPVVILKAVEQVLAGVPESAASPSEQFDQEH"
     gene            complement(20752..>21183)
                     /locus_tag="VGE92_09660"
     CDS             complement(20752..>21183)
                     /locus_tag="VGE92_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1314135.1"
                     /translation="DMSSNPELSILLIEDDDVAAEAVVRGLRKHSISYPIVVAEDGRE
                     ALDILRGTHATQKIEKPYLALLDLNMPRMSGTEFLQELRVDPQLHATVVFVLTTSGAD
                     KDRARAYQHNVAGYMVKSALGPQCSRLAKFLCEYQATVLLP"
BASE COUNT         3995 a         6576 c         6805 g         3807 t
ORIGIN      
        1 ccggccgcga ggcggcggcc cgcctgccgc cgggcattgt cgtcgccggc aagaccggca
       61 catcctccga tacgcgcgac agttggttcg cgggattcac cggcagctac ctcgccgtgg
      121 tctgggtggg ctacgacgac aaccgcgtca ccggactcac gggcgccgcc ggagcgctgc
      181 cggtgtgggc cgacaccatg gcggcgctca gctcgacgtc ttttcaaccc gtgcccccgg
      241 aactcatcga ggagcgctgg atcggattcg cggatggact ggagaccacg ccggcgtgca
      301 gcgcggacgc cgttgcggtc gcggttccga ccggtacggt gctgcctgcg aacacgaatt
      361 gcggtccgcg gcccgcccct ggctcgccat ccggcacgat gggtgacaag atcaagacat
      421 ggctcaagca catcgttcac tgacgctcgc cgcctgcttg gccgcggccg tgctgctctc
      481 cggttgtccg gcgcccgagc cgcgccccat gccgccgccg ggcacgtcat ttccggaatc
      541 gccgccgtcg gttcccgcac agccggcacc ggcgccgcca cgtgaaaatc atctgtctgc
      601 tgccacgcat tctctggtga cacaggcacg cacgctcgag acgcacggcg atctcgacgg
      661 cgcctcctcg accctcgatc gggccctgcg catcgagccc aataatccgc tgctatggat
      721 cgagatcggc cggctacggc tcgccgaaaa cgatgcgcat caggccgagg gctgcggccg
      781 caaggcgctg gtgctcgcga gcggcgatcg gcgcacgcag gcgcaggcgg gccacctgct
      841 cgccgacgcg ctgcgcgcgg agcagcgcaa tcaggaggcg agcgaggtcg agcatcaacc
      901 gttcatgcag tgatggatga gtgagagtcg cgcgccgact cctgtggggc agcctctccg
      961 gcacggcgtt gctcagcgcg gcgctgatcg cattcaacac cttcggcctc gccgcgagac
     1021 tgtgcaccat tcccggagcg caacccggag tctccgatgc gtgcggcaga ttgggccttg
     1081 gcggacggcc gattcgttct gagcgcctcg catgggagtc gcggaccccc ggaagctgtc
     1141 aggcgttgcg cgaacacatc agtcgttttc ccggcggcgc ctatcgcagc gaagccgcgg
     1201 atctgctcac cgctcgccgg acatcgacca tcgagacctg ggagccggcg agccgaacgc
     1261 tcgccttgtt tgagccggca ggctccgccg ccaaggacga ggtgattgcg caagcgcacg
     1321 ccttggaaag cgcgaaagcc gatgccgagc ggctgtgccg cggatttggc gcgggctcgc
     1381 tgtatcgctt cgtgtccgca tccgccgtcg ccgagcgctg gtcctgcact cgcggtccgc
     1441 aaggaactgt ctgcggcttc gatgggcatg cgcaatgcga attgagcgta agccgccgca
     1501 cagaacgaga acagtgcggc tgattgggat cgcacgcagc gctgccgcaa tcgcgctcat
     1561 gccagccggc acgtgccacg ttcgacaacc aaaacgacaa atagttgcct caggccgccg
     1621 ccgcgtacca gccgcattca tgcggccggc ggagtacaat ccgtcttgag ggccgtcaca
     1681 ttcgacccgc tcgaggaggc gctaatgcga cgcacatcgc acattattct tttcggactt
     1741 gcaggcaagt tcaccttcta cagtttgcgc cgctatgaat aatgcgttac acatattatc
     1801 gggagaggcg gataaggaaa gaatttccgt ttggcgcacc ttgtgaggtc taaccgtgac
     1861 aacgagcgca ttgatttttg acgatttcaa gacttcctac gaaaagagcg cagcagcgaa
     1921 cttcacgctc gctgagtatt tcgagctgtg ccgcaacgag cctctgactt acggactcgc
     1981 ggcggagcgc atgatcaagg ccatcggcga gcctagactg gttcaaacca aatccgatcc
     2041 gcggctgctg cggctgttcg gcaatcgaac tctcaagtac tacgacgcat tttcagactt
     2101 ctacggcatg gaaggcggca tcatcgagga aatcgtttcc ttctaccgtc acgccgcgca
     2161 aggtctggaa gagcgcaaac aggtgctcta cctgctgggc ccggtcggca gcgcgaaaag
     2221 cacgctggcc atcaaactca agagcctgat ggagcgctat cccatctaca cgctggtcga
     2281 tggcgatacg ggcgagcgca gtcctttgct cgagtcgccg ctcggcatct tcgatccgga
     2341 gaaattcggc gcgcggctcg aaggagaata cggcatttcg ccgcgctacc tgacgggcat
     2401 ttcgagtccc tgggtgcaga agcgtatgcg cgcctacaac ggcgatctga cgcgcttcaa
     2461 agtgcagcgc ctctacccct cggtgctcaa ccagctggcg gtcgcaaaga ccgagcctgg
     2521 cgatgagaat acccaggata tcagctccct ggtcggcaag gtcgacatgc gcaagctgag
     2581 caagctcggc caggacgatc cggacgccta cagctattcg ggcggcctgt gtctgacgac
     2641 tcaggggttg ctcgagttcg tggaaatgtt caaggcgccg atcaaagtgc tgcatccgct
     2701 gttgaccgcg actcaggagg gcaatttcaa gggcaccgaa gggttcggtg caatgcccta
     2761 ccagggcacg gtgctcgcgc attcgaacga gagcgagtgg aataaattcc gctccaatcg
     2821 cgacaacgaa gcgttcttgg accgcatttt catcgtcaag gtgccgtact gcctgcgcgt
     2881 gaccgaggag gtgctgatct atcaaaagct attgcgcgaa tcgaccttgt cgaacgcgcc
     2941 gtgcgctccg ggtacgctgc cgatgttggc gcgctacatg gtcctatcga ggctcaaggc
     3001 gccgtcgaac tcgagtatct attcgaagct gcgcgtctat gacggcgaga atctgcgcga
     3061 gtcggatccg caggccaaga gcgtggccga gtatcgcgaa atgggcggca gcgatgaagg
     3121 catgaccggg ttctcgacgc gctatgcctt caagctcatt tccaaggcat tcaaccgcga
     3181 cgcatcggag gaatcggcgg atcccgtgac tctgctcaag gtgatcgaag agagcatcgg
     3241 cacctcggat ttcagcccgg atctgcagaa acagtgcacc gtgcatttga aggatttcct
     3301 cattcctcgc tatctcgagg aactgaccaa ggagatacag gcggcatatc tggaatccta
     3361 tgccgactac ggccaggaac tgttcgatcg ctacgttgct tttgccgact tttggattca
     3421 agaggaggtg tacgtcgatc ctgcaaccgg cgtcgccttc gacaaggctg cgctcaattc
     3481 cgaactggaa aagatggaga agcccgcggg catcggcaat ccgaaggact tccggcacga
     3541 aatcgtcaat cacgtgctgc gcgcgcagaa taagagcggc ggtgataagc tggtatggac
     3601 gagctaccaa aaactccgcg aagtcatcga gaagcggatg tttgcggcaa ccgacgatct
     3661 attgccggtc gtttcctact tcgccaaagg caacgccgag gatgagaaga agcatgaggc
     3721 gttcgtggcg cgcatgaagg cgcgaggtca cactccgacg caggtgcgcc gtcaagtcga
     3781 atggtggacg atggctcgac gtcagtgagc cgacacccgc tcgaggtact cgcaccgtga
     3841 caagcgtcat tgtcgatcgg cgacctgata agggcaagag cgtcgccaat cggcagcgcg
     3901 tattgaagcg gctcgacgga gcgctcaagg cacaggtcga caaaatgatc gcgcggcgca
     3961 aactgcagga ccacgacaag ggcgtcgaag tcacgatcga gcgcaaggac gtcaaggagc
     4021 cgagtttcac gctggacggc gccacgggct cgtcgggtcg ggtcattccg ggcaacgacc
     4081 gctaccgcgt gggcgatcaa ataccgcgcg atcccgcgca ggcccgaggc cgaggcgccg
     4141 ggtccggcac cggcgagggc tcctccgacg acgaagatgt gtttcgcttc gcgctgtcgc
     4201 gcgaggaata cttgtcgctg ttgttcgatg agctggaatt gccggcgctg gtgaagaaag
     4261 acctgttgga aatcgacgag aaccgatttc ggcgcggcgg agtggtgcgc tacggcagtc
     4321 caggaacggt atgcgtcgcc cgcaccttca aggcgtcgat cggccggcgg gttgccgccg
     4381 aagcgcagca cgaggaggct ctcgatgcgg cggaggccga gttgctggcc gcgcgtgcga
     4441 gcggcgtgca ggcgcaggtg caggctgcgg aggaaaggct tcaggaagtg cgtcaaggcg
     4501 gcgcggcgat cccgttcttg gatcccgttg acctcaggca ccgcaacctg gtggaagtac
     4561 aggcgccgcg cagtgcggcg gtgatgttct gcctcatgga cgtatcctcg tccatggatg
     4621 agtcgcgcaa ggatctggcg aagcgtttct ttacgctgct ctacctgttt ctcagccgca
     4681 aataccagaa ggtcgaactc gtcttcatcc gccacaccga tcaggcgcag gaagtcgatg
     4741 aggacacctt cttcaacggc acgcaggcgg gcagcacccg ggtcctgtcg gcgctggaaa
     4801 aaatgcacga gatcatcggc ctgcgctacc ccgcgtcgca ttacaatata ttcggcgcgc
     4861 aggcgagcga cggcgattcc ttcggcgccg actcgagcga gtcgtcggct tacctcaagt
     4921 cgcagctgct gccgtcctcg cgttacttcg tctacgccga agtgggcgaa aagtcggcgc
     4981 cgggcacgac ccggctgtgg gcggcgtacg atgacatcga gtgcgatcat ttcaacatgg
     5041 cgacggtagg tgcccgcgac gaggtctacc ccgcgctcgc caagctcttt cagaaggagc
     5101 agccggcctg atgaaggaac agcgcatgct caccggcagc gaatggacat tggacgacct
     5161 gctcggcatc gagcaaatct gcgcggccca cgcggagcgt tacgggctcg atacctttcc
     5221 caatcaattg gaagtcatca ccagcgaaca aatgctcgac agctacgtgt cgagcggcct
     5281 gccggtgaat tacggacatt ggagcttcgg caagcattac ctgatcgaaa gcggcaagta
     5341 tcgctcgggg gcgaacgcgc tcgcctacga aatcgtgatc aacaccaatc cgtgtatcgc
     5401 cttcttgatg gagggcaatt ccctcgcgat gcaggcgctg gtcattccgc atgcatgctt
     5461 cggccacaac agtttctcca agggaaatta cttattcaag caatggaccc agccggacgc
     5521 cgttctcgat tacatgttgt tcgcccgcaa ttacatcgcg gattgcgagg agcatttcgg
     5581 gctcgatcgg gtcgagcgga cgctcgacgc gttgcacgcg ctgcgcgatc aaggcctcga
     5641 caagtacaag cggccgcgca aattggacac ccgcaaggag cgcgccgagc aggcgaagcg
     5701 cttgtcggtg gcgcaagaaa ccatgcgcca ggccgagtac tacagcgttg tgccgcatgc
     5761 ggccaaggcg gagagcgccg ccgcggtgtt cccgccgagc cccgaggaga acctgctgta
     5821 tttcatcgag aagcacgcgc cgaaactcga gccatggcaa cgcgaactgg cgcgcatcgt
     5881 gcgcaagatc gcccagtatt tctatccgca gcgccagacc caggtgatga acgagggttg
     5941 ggcaaccttc tggcaccatc ggctgatcaa cgatatgtac gatgcccggc aaatcgacca
     6001 gggaatcctg ctggaatgcc tgcactcgca taccaacgtc atccgccagc acgaatactc
     6061 gcgattgaac ccgtacgcgc tcggttttgc cgcatggcac gagatcaagc gtgtctgcga
     6121 ggcgcccacc gccgaggatc gcgagtattt gcccgacgtc gccggcaagg actggctgga
     6181 aacctttcat gacatcatgc agaactaccg ggatgagagc tttttgctgc agttcctgac
     6241 gccgaaactg gtgcgcgagt ttcgcatgat gaacattcaa accgccgaag ggctcgaaca
     6301 ttggaatgtc gagaacacgg cgggagagga gggatttcag gccattcgca gccagttggc
     6361 ggccaactac cgcctcgagt cgatgatgcc ggagatttcc gtggttcgct atggacgcga
     6421 taccgacaga aagctcctct tgcagcacca ggtctacaac ggcagaacgt tggacgaatc
     6481 cgagaccgaa cagacgttga agcatctgcg cgcgttgtgg ggattcgagg tggcgctcga
     6541 ggcggtcgac cagaacgggg cggttctcaa gacctattga gcgcagcaat gccagcatcc
     6601 ctgcgtcctt ccatgggtgc gactccctat gcggattcct cgggaagcgg cgtgactttc
     6661 cgcgtctggg ctccgttcgc gagcgcgctc aacgtggccg gcgatttcaa tgcctggtcg
     6721 ccgtcggcga ccccgctgta cgccgaaggc aacggctatt ggtcggtcga taccccggcc
     6781 gcagcgctcg gttcgcaata caaatttgtc gtgaccggcg gcggcgcgac gttgtggcgc
     6841 gtcgatccgt atgcccgctg catcagccgc tcgaacggcg tcctcaattg ccgggtcgcg
     6901 gccgccgatg agtcttactc gacaccgggc tattcgactc cggcctggga tcaactggtc
     6961 atttatgaaa tgcacgtcag gacctttctc tactgtcccg acggcatcga tggcaagggc
     7021 agtttccgat cggcgatttc caagctgccg tacctgcgcg agctcggcat aaacgccatc
     7081 gaactgctgc cgatcggcga gttcatcggc gatatatcgg ccggttataa cccggcgtac
     7141 atattcgcaa tcgaggacga atacggcggt cccgatggct ttcggcaatt cgtcaacgcc
     7201 gcgcaccaac aaggcatcgc ggtcatcatg gatgtggttt acaaccactt gggcgtaccc
     7261 gccgccgaca tgtgggtatt cgacggctgg tcgcagaacg gcaaaggcgg catctatttc
     7321 tacaacgact ggcgcagcag caccgtttgg ggcgagaccc gcttcgatta cgggcggccc
     7381 gaagtgcgcg cgttcctgcg cgataacgcg ctgcgttggt tggagtcgcg ctttgccgat
     7441 gggctgcgct gggattccac ggggtccatc cgcaacgtct acgatgccaa tgacgatacc
     7501 gcgaatgaca tcccggaggg ctggagcctc atgcagtgga tcaacggact cgtggcgcag
     7561 agccagccct ggaagatctg catcgcggag gacatgcacg agaatgagtg gatcaccaag
     7621 gatccaggct cgggcggcgc cggcttcggc gctcaatgga gcagcgcttt tgtgtccacg
     7681 gtgcgcgccg ccctcgtcgg cagcgacgac ggccgccgcg acatgtacgc ggtcgcggcg
     7741 gtgatcgctt cgcgctacaa cggcaacgcc ttccagcgcg tcgtcttcac cgaatcgcac
     7801 gatgccgatt cgaacggcga tcagcgcgtg ccggagatga tctggcccgg caacgcccag
     7861 agctggtatt cgaagaagcg ctcgacgctg ggcgcggccc tggtgatgac cgtgccggga
     7921 atccccatgc tattcatggg acaggaattt ctgagcggcg gatggttcaa ccccgacgcg
     7981 ccgctcgatt ggtcgaatgc cgaggcattt ccggggatca tcttgctgta tcgggatttg
     8041 atccgcttgc gccgcaattg gtacgacaag acgcggggat tgagcggcca gcatgtcaac
     8101 gtgcaccacg tgaatcagag cgacaagctg atcgcgttcc accgctggga acagggcggc
     8161 agcggcgacg atgtggtcgt ggtgctcaac ttcagcaccc aggccttcgg catctatacg
     8221 atcggcgtgc cgcgggaggg cacctggcgg gtgcgcctga actcggattg gagcggctat
     8281 gacggctcgt tcggcaatgc gccgagcgtc gatgccgcgg cgcgtggcat cgcctacgac
     8341 tcgatgcccg cgagcgcgag cctcgccatc gggccctaca gcgctgtgat tctttcacaa
     8401 gactgaagta ttgagatcct ggccttaaca tgcgctaaat tgcctacgga tcgatcgcca
     8461 aattgccgaa acgctcgctg cagcggccgc ggcttgaatg ccgccgggca ggcgatattt
     8521 cattcagttc tcgctaggga tgcgaatgcc gctcacgccg gatcgaatgg caacgatgag
     8581 ccgcctgctc gacgcggctc tgcccttgga tgaggccggc cgccggcgct ggctggaggc
     8641 gcttgcgcct gaatatcacg accttctccc cgagttgcgc gaggcattgc tcgccgagtt
     8701 ctataccgcg ctgccgcccg ccaccggcgc cgagcgcggc gctgcggcgg cgaccggact
     8761 tgctcccggc gcgcatgtgg gtccgtacga gttgattcga ttgctcggcg cgggcggcat
     8821 ggcggaagtt tggctcgcga agcgcgccga cggcgttttc aagcgcgagg tggcgctcaa
     8881 attgccgctg ctgacccggg tgcgccaaca tttcgagcag cgttttgcgc gcgagcgcga
     8941 catcttggcg ggcttggagc atccgcatat cgcgcgcctc tacgatgcgg ggatcgatcc
     9001 cgacggcctg ccttatctgt cgatggagta tgtgcagggc cggctgctga cggagtggtg
     9061 cgatggcgaa tgcctcgaca tccgccagcg cctgcaattg tttctgcaag tgctcgaggc
     9121 cgtgcaatat gcccacgatc gccatgtcat tcatctggat atcaagccct cgaatattct
     9181 ggtgagcgac accggccaat cgcggttgct ggatttcggc gtggcgaagc tgctcgagga
     9241 tgacgcggcg accggacaga cgccgctcac caaggtgtac ggacacgtga tcacagcggt
     9301 ttacgccagc cccgaagtgg tgcgcggcga tccggtcgat gcccgcagcg atatctattc
     9361 gctcggtgtg gtgctgttcg agctgctcac cggcgtgcag atcgcggcct tcggccggat
     9421 tcgccaggcc atcaccgcca tgggcaaagc cgtcgagttg gatccgctga acgcttacgc
     9481 gtggggcaac atgggattgt tcctgaccat ggatcgcgac tatccggcgg cgcgccgcgc
     9541 cctcaatcgg gcgttggcca tcagtcccga cagcgatgcc ttgcattttg cgctcgggga
     9601 actggacatg ttggagggcg aattcgcgca cgcctcggcc gagttcgcga aagaaggcaa
     9661 tgagggcgcc cgccgcatga gcgacgcgct gctcgagcat gcgcgcggca acgagaccga
     9721 gtcgcagcat gcgctcgacg acctgatcgc ggctcacgcc gccgacatgg cctatcaagt
     9781 cggcgacgtc tatgcgtggc gcggcgacaa ggacgaggcc ttcgagtggc tggaacgggc
     9841 atatcggcag cgcgacagcg gcctgaacgg aatcgtctac gatccactgc tcgcggggct
     9901 caaagatgat cctcgctacg gcaagctgct caaggagttg gaattgtcca actgacccct
     9961 caggcggcta gagcgtacgg gcgagcacga tgagcgcaag cccggtgagg aaactcgcga
    10021 acatcgccga cagcaacgtg ggtgtaccgg gcggggcggt gcgaaattcc ttccaaatga
    10081 agacgcccca gaaggcagca accatggtgg cgccctggcc gagtccgtag gaaatggcaa
    10141 agccggcgcg cccggacgcg atcaagttca gcgacatgcc cactccccag atcacgccgc
    10201 ccaaaatgcc cgtcatgtgc gtgcccatgc tgccggcgaa gtagtcgcga aagctgaccg
    10261 gggcgccgac gaacggcttt ttcatggcga tcgtattcca caggaagctg ctcaggaaaa
    10321 tgccgacggc aaacacgaac accgccgcgt aggaacccat catgcccgcg gttgcgtgag
    10381 cgggatccgc gtacatcgcc gcggcgacga agcgatagaa catccccatg aaaacaccgc
    10441 aggcgatcga cagcaccagg cccgtattgc tgcgcttgcg gcccgcgtcg ggtgtcgcgc
    10501 cgggcaaccg gcgataggcc gccgcatcca aaatgatggc gagcgtcacg agcaaaacgc
    10561 cggtcgcgag gagcgtgacg ttgcccaccg gtgccgcgag atagttcacg atcacgccca
    10621 ggatcagcgc aagtccgatc ccgacaggaa atgccacggc cattccggca atctcgatcg
    10681 cggccactag caggatattg gccagattga atatcgcgcc gccgagcaac gccgatccga
    10741 gggaggcgcc cgaggcctgc gacaagtcgg cgaggaaggc gcgtccttcg ccgccggtgc
    10801 tgccaagcgt caggccgaag atcagcgtca gcagcagaac gcccagcgtg taatcccagt
    10861 aaaagagggc aaatccccaa ttagggcgcg ccagtttctt ggtggtcgcc caggagcccc
    10921 aacaaaacat ggtgagcacg caaaatacga cggccaacgc ataactgtgc acgacgaaca
    10981 tggcatcaat ccttggcgcg gcgccgcgcc cattcggcgt cgaaatcgac ccgccgcggg
    11041 aatgatttct gggttccaac gcgggtcgtc gacaaggcgg cgtacaggtt cgcgcgtgcc
    11101 agcgcttccg gttcgcgcac gccctccgcc aggaacaccg cgaaactgcc gataaaggcg
    11161 tcgcccgcgc ccgtggtatc ggtggccttg accgcgaacg gggcgaccag cgtgtgccct
    11221 tcggcgcccg ccagcagcga gccgcgcgcg ccgagcgtta cgacgatgcg ctgaaagcct
    11281 aggctcaaga ggcgtgcggc tgcggccgct gcctcggcct cggttcccgc cgcgaggccc
    11341 gtgatctttt cggcttcggt ctcgttggga atgaagtagt cggcgcccgc caactcggcg
    11401 aggttggccg ggacggccgg cgcgggattg acgatgcagc gaatgtggtg cgcgcgcgcg
    11461 aagcgaatgg tgtggtagac ggtgtcgagc gggacttcga attgcaggat gatggcatcg
    11521 acgcgcttca actccgccgc cgccgcgtcg acgtccgcgg gccttaagca gtcgttggcc
    11581 cctttgacga cgatgatgcg attctggccg ttcggctcga cgaaaatggg cgcaacgccg
    11641 gtcgacgcat cctcgacgat accgacgtgc cgcgtgtcga cgccgaaggc ggcgaaatta
    11701 tcgatcgttg cctgcccgaa caaatcgcgc ccgaccttgg ctaccatcag cacgtccgcg
    11761 ccgcagagcc gggccgcaat ggcctggttc gcacctttac cgccgaaccc caaatcgaaa
    11821 ctcttgccga acagcgtctc cccgggacgc gggaacacgt cggtaaacgt cgtcaggtcc
    11881 gtgttggcgc tcccgaccac ggcaatgcgg ggtgagttag gcattctttt ttctcgcagc
    11941 gggcgctcgc ggggtcgcac gccgcccaag tttagcatgg cgaggacggg cgcgagcggc
    12001 gcccccctcg gccgcttgtt gtcgcgggta cacaccgatg tgatgcttga acgccgcaaa
    12061 ataaaccagg acaccgttca agtcgaaggc cggcattcca tagctgatcg tttcgtgcgc
    12121 cgccggcgcc gcgggacggc gagccgcgct cattcgcccg acgccgccag cgcggccagc
    12181 aattccttga acaacgcgga tgcaccgatg cggaaattgg ccgccgtggc gctgccctcg
    12241 ccgaacctcg cgtcgtagcc gcccaagtaa atcaatgcat cgtcgaaggt gcggatgccc
    12301 ccggatgctt tgaaaccgat cgcctggccg cgcccggccg ccgcggcgat cacgtcccac
    12361 aagacggcgg ccgccgcggc ggttgcggcc ggttgtgtct ttccggtcga ggttttgaga
    12421 aaatgcgcgc cgccctcgac ggcgatctcg gcggcgtgcc ggatcgtatc ggcgccgccc
    12481 aattggccgg tctccaagat caccttgagc agcgcgcggc ctgcgcaggc ctggcgacag
    12541 gcgcgcacca gcataaggcc cacgccggca tcgccggcga gcagcgcgcg gtagggaaaa
    12601 acgacatcga cctcggcggc gccggcgtcg accgcggcgc tagtctcccg cgccgcgccg
    12661 gcggggtccg cggcgccgcc cggaaaattc gcgaccgcgc agacggcaat gccgctgccg
    12721 cgcagcgact cgagcgccgc cggtatcaat cgtgcccaga tgcaaaccgc cgccggtttg
    12781 accggggcgc tgcgtgcaag cttcgagagc gcgtgcaccg cggcggcatc gtcggcgtcg
    12841 ttgaggctgg tcaaatcggt gagcgacagc aggcgccgca ccgattgggt tctcgtcatg
    12901 caggctttct ccagcaggtg aagttttctc cgtgtcgttg gccgagttga cttgagccgg
    12961 ttcgctggtt agaaccatag taccccggga tcgttgatac tcgccgcaca aaacgacaat
    13021 caaggatgtg acgccatgcc gctgaagaca ctagcagccg ccctgttgtg cgtggtcgcc
    13081 gcggtcgcgc gcgcggcggc cgagcacacg cccattgaag tcaaagtggt ggtggtcacc
    13141 atgttcgaga tcggcgcgga ctcaggcgat gcggccgggg agtttcagct ctggtacgag
    13201 cggcaaaagc ttaaccgtcg ctacccgttt gcgcactatc acgatttatt cctgaatcga
    13261 cacagcggtg tgctcggcat ggtcacggga gaaggcagca tcaactcggc gagcgcggtg
    13321 atggagttgg ggatggatcc gcgcttcgat ctgtcgcatg cctactggct ggtggccggg
    13381 atcgccggcg tcgatccgga cgcggcctcc atcggctccg ccgcgtgggc cgagtatctg
    13441 gtcgacggcg atctggcata tgaaatcgat gcccgggaga tccccgcgga ctggcccacc
    13501 gggtatttcg cgcgcaatac ccagaagccg tacgaccccg agaagcctgc gcccagcggc
    13561 gaggtgtttc gcctcaatcc cgacctcgtg cagtgggcgt tcgaactcac gcgcgcgatc
    13621 aagctcgacg acggcaagga actgcaagcg gcgcgcgccc agtacacggg ctatccgaac
    13681 gcacaaaagc cgccgttcgt gctcaaaggc gacacgctgt cggccatgac gttttggcac
    13741 gggaaaattc tcaatgagtg ggcgcatcgc tgggtcgact actggaccga cggcaagggt
    13801 cgattcgtga cctcggcgat ggaggacacg gggaccttgt cgtcgttgac gtacctgcac
    13861 ggcaccggca aggtcaacaa ggaccgcgtc atggtgctgc gcaccgccag caactacacc
    13921 gtgcagccgc ccggcgtgag cgccgcggat caccttctca aggagaacgc gggctacgcg
    13981 ggcctgcatg cgtcggtgga agccgcctac cgcgtcggtt cggccgtcgt cgatgagatc
    14041 gtcggcaaat ggtccgtcta tcgtgaacac gcgccgcagc ccggcgcgcc gcgctgagcg
    14101 gggtctattc cgcgggcaga cacagctggt aatgccggca caggaagcca tagctcaccg
    14161 gccgggtcgc actcaaggag ctccagccct tcctttgaaa actgcgcgcg aaatcggcga
    14221 cgcgcttgtc gtagtcggcg agcagccgct gcttggtggc ttcgtcgaca aagccgtatt
    14281 gcagggagtt gcggccgagt ttgacgatct cttcccacga cagattgaac tccttcaccg
    14341 ccacgtagaa ttcatcgctc aggttcgaat cccacatgcc gcgatcgtcg gtggacaagg
    14401 cgacgggaat gccggtccgc aagtactcgg gaaacgggtg ctgggaatag tccttgacgt
    14461 actcgagcag cagattgctg atcaggttga tttcgaccat gaacggtccg tagcgcatgc
    14521 gcaccagggt gtccggatcg ctgattaggt tgacgccgtg gccgatgcgt tccgcgccca
    14581 gcagcagagt atcgcggaca tggaagttcg gttcatccgc ttcgccggcg tgaattgaca
    14641 gatgcatgtc cgggtattga tggcgcagct cgcgcagcac cggcaagaac cgcagcggat
    14701 agcctctatt gttgtcctcg cgtcccgcca tgttcacgcc gacgtacaga tcgcggtagc
    14761 ggtcggtgat cgcgtacagc gtgcgcaaat gctgctcggc gtcgggcgcg tagcgcagca
    14821 gcacgttctg aaaacgcacc tcgactccgg tcgctttcgc cgcgggcgag gccaggcacg
    14881 cacgatagat gccgacgact tcgtccacgg cgatgggcgt gccgtcgggg cgcacggcgt
    14941 tctcgagacc ttgctgcgtt tcgagatacg cgaggccctc cttgccgaag gcctgcatgt
    15001 tccgcagcaa gatgtcgcag atcaggtagg gattgcgcag catgtcattg atgcgcgccc
    15061 aaatggcctc gaagaattcg ttgcgtcctt cgtaaggggc atcgagacgc atgctgttga
    15121 gccacgcttc cttctcgcgc gcgtcgaggt cctgcaagcg cttgaattcc gacttgccgc
    15181 aggcgtcgag ccgatcgaag gtgctcgcct ggatgatctt gaagagcagc aatgccttgg
    15241 atgccccgaa ttcatcgtgc ccgtagggcg cgcaattctc gattcgcacg cgcgtgtaat
    15301 aggtgtagcc gcgctgctgc gcggccatgg ccgcgtccca aaaccattcc gagcggacgg
    15361 cgccggcgag atgattgtgc aggtcgccgc ctttcggcaa cgcatacagg aagcgataca
    15421 attgctcagg cgtcgcgctc gccttgacgg cgtcgaacca gggcgctgcg gcgtggctgc
    15481 gcgtgttgag cgccagacaa cacagcccgg cgagcaggga tcccagcgct gcgcgcataa
    15541 cgcttctcac tcggcgtgac cgggtgccgg ccgggtgaga agatcgaggc tcaaggcttc
    15601 gaatttcttc aggtcgatat cgaacaccac ctgcaccggc tgctcgccca aattcgggcc
    15661 ctcgccgggc gaccaactca acgtatcgcc gtagcctgcg gtgaagctgg tatcgacgtc
    15721 gaccagcagc gtcttgctct gcgtgacgat gccgggatcg agccagacgg cgacggcgag
    15781 ttcatcccac atggggtagg gctggccgaa tttttcgatg taggcatcga acggcgcctt
    15841 gccccggccc actcgatgct gcatttcggc cgtgaacagc gtcttggtgg tcggatcgac
    15901 cggaacctcg gtgaccttgc gccagggttc atgcaacacc atcgacgccg cttcggggtc
    15961 gaagcgcatg ttgaattcgc ggcgcggcgc gttggcatat tcggctgcga atgcattgtc
    16021 ggcggcggtt ggattgaagc tgccgcccat gaacaccaat tcctgcgcga ggccgctcca
    16081 tttcggatcg agccgcgccg cgagcgccag gttcgtcagg ggtccggcgg cgatgagggt
    16141 gacttgaccg gggtagcgcc gcacttcgcg aatcaaaaaa tcggcggccg actcggttgc
    16201 ggctttgatg tgcgccgtgc cggccggcga ggacggcacc aggtaaggat cgtcgggatg
    16261 cggcacgcgg cggttgacgc cctggtcggg ccaatgttcc gtccacgcgc ccttgtaata
    16321 cagtttgccg tacgcaccct cccaggctct ggtgcgcgcc ggcgaattca agagcggcag
    16381 cacggcgccc ggaaccaccg gcacgtcggt gcgtccggca atctcgagca gccgcagggc
    16441 gtgatcgacc tcttcgtcgc gccagccgtc gccgctcgta atggtgatgc ccaatacctc
    16501 gacgtcgctc gactgcaaca gcaacaggat ggcctgcata ttcgagccgg ccggccccaa
    16561 ggcgtcttga tcgatgatga ctttgcgttt gtggccggca tcggcggcct cggcggtgcc
    16621 gctgattgta ctcaagagcg ccgcaacgag aatcatggct aacgcaccgg tgcgcatgag
    16681 cgcattacag cagtttttca tgcactaagg gcgtgcatct cggtcgagag agtttttttg
    16741 aattgcggaa ctgcgcgtgt gccgtttata ttcatgacac gaccgcgcca tcttcccacc
    16801 tttccggcgt ggtcaaggag gcagagtgat ggttacgaaa cgcgtatcct cggtgaagag
    16861 cgttccctcg atgaagaaga cagtgatccg atcgaccgtg gcgagcgcca tcgacccgga
    16921 cgttcggcgc aagctggtgg cggctgaagc gtatttcctc gcggagcgtc gcggcttcac
    16981 ggcgggcaac gaactcgagg attgggtagc tgccgaagcc atggtggatt cgaagctccg
    17041 gcagttgcag gtggcttgat gcccaacggc tgatcttccc tcgggcggcg aaggccccgc
    17101 atcatcaggg cggggtattt tccggaaagc gcaaggtttc gaagcgcttc gcctcggcct
    17161 cgcccaattt tttctgtgcg cgaatctcgt tcacgatctc gagcgcggtc ttggcgcacg
    17221 gcggcaactc ggcgtcgcac ttctgcagcg gggtcacgcg ctcgccccag cggatcagat
    17281 gggcggacaa cgtcaggccg ccgccgaatg caggcatcag cacgaggttg ccgggcgcga
    17341 cgcggccttc ttccacggcc tcgaccagcg cgacgggcgc ggttgccgaa ctcatattgc
    17401 cgtacttctg caccgtcaga tacagtttgt ccgggccggc accggcgcgc ttcgcgaccg
    17461 catcgatgat gcgcaggttg gcctggtggg gcaccaccag cgcgatgtcc tgcatcgtca
    17521 cgccggcgcg gcgcatcacg tccaggctcg cttcgctcat gccttgtacg gccttgcgaa
    17581 agatttcctg tccgtcgaag tcccatcggg tctgtccgaa ctgtacgccc agattggcgt
    17641 acatcgtgcc catgccgcgc acccgcagga tttgtcgcga gtccgcataa cattgcagct
    17701 tctcggcgat cacgccggat tcatgctcgg actcgcgcag caccatcgcg gccgcgccgt
    17761 cgccgaacaa cacggcgaca ttgcggttgt cccaatccat gtactgcgaa atgagttcga
    17821 cgccgatgat caacgcgttc ttgacgatgc cggtcttgat catcgatgag ccgatggaca
    17881 gtccgtacat gaaactggtg catgcggtat tgacgtcgaa cgcggccgcc tgagtggcgc
    17941 cgatgcgctg ctgcacgccc gaggcgctat tcggcaccgt ttcatcgttg ctgcagctgc
    18001 cgtagatcac caagtcgatg tccgcgcccg acatcccggc gcaggcaatg gcacggcggg
    18061 ccgccacgat ggcaagctcg atgcccggca cgtgggagat gcgcctctgc ttcatgcccg
    18121 tgcggctcgt gatccactca tcgtccgtgg ggaggaaggt cgccaggtcg tggttcgtca
    18181 gaattgacgg cggcaggcat ttgccccagc cgacaatggc agcgtgagtc aagagaactc
    18241 cccaaggcgc ttctgtttgt cagccattgt cgccgatatg gcggcaactc gcacgtttag
    18301 gcgggaacgg cctcaggagc gcggaatggc gtgtccgctg gtgccgccgc gcgtgaattc
    18361 gaactgcaac agccactcgc gcgcgtccgg atgatcggcg gcaacgaaac gccacttgcg
    18421 ggcggcttcg ctcgccgcgc gggcgaaata cttgctgttg ccgcgatttt ccagcgcctc
    18481 gtcgacgaca ttcccggagc ggtcgacggt gacgcgaacg gtgaccacga tatcgccgcg
    18541 aatcgacgcg cgggcaccgc gcgaaatgtg cggaatttct tcatgacgca ccgaccgcga
    18601 agggtcgacg gcgggggcca cggcagacgc cgacgcggca accggggcag gcgcggaatt
    18661 ctgcggcacg gcagaagccg cggccgattg cgcgaccggc gccggcaccg gcaccggggc
    18721 agtcgaaacg ggggagccgg gatgttgatg gtgccagaca gcccaaacgc ttcccgccag
    18781 gacgagaccc gccacggttc cccacacggg gaggcggcga tcgggaaact tggacaacgc
    18841 gtccggattc ggcgcccgca ccagaagctc cgtcagcgac agttccggct gagccgcgga
    18901 cgccgcggcc gattgtgcga ccggcgggat cggcgggatc ggcggcaccg gcccgaccgg
    18961 tgtgaccggc gcggattcgg ccccgtcctc ggccggggca gcgggtttgg ctgcctccgc
    19021 ggggcagggc gacgggtcga attggacctt caggtcgcta atggtcggcc gcagagcggg
    19081 atcgcggttc aagcaacgct cggtgacccc ttcgaagcct gcaggaatcg gcgccggcgg
    19141 caggacggca atttcggtgc gttgatcgag gcccgcaggc agacgctgag tcagcgcctc
    19201 gaccatggtc acgccgaggc cccagacgtc gcccttgtcg tcgatgccgc cgatttttgc
    19261 ctcgggcgga tcgtataccg aacgcttggc gatgctcgga cgggatctgc cgaccggatg
    19321 gatgttgtcg ctcgataatt tcagttgatc gtcgatgacc aggaagttcg ccggtttgag
    19381 ctggccatgc actaaatctt tggcgtgaag gtaggccagc gtatcgagcg tcgggcgcag
    19441 catctcgcgc acttcatcgg gagtgagagc tcgcttgggc agaatctgcg cgagcgtctc
    19501 ctcggcatat tccatcacga cgaataggaa cgggtgaccc ccgagttggc aacgacccgt
    19561 atcgaacaag cggatcaagt ggggatgggc gagggcagca gcccttttcc aatactcgag
    19621 ctgggtgtcc gccgcggcgg gatcggccgg caccagtttg atagccgcat tggccaggtt
    19681 ttgcggcttg tattcggtga ggaataccac gctgtggttc gacctgccca agaaacggcg
    19741 caggggcagg accccgttga tgacccggtt ttcccacttc gcccagactt cgctcatcat
    19801 ttcgacacgc tttcaggatg cccaggcgaa gatatcatct gcaggggccg gcggctgcgg
    19861 aatcagctct cagatttgag ggctgccggc ctcgtaaagg cggctcgcca acgagccgac
    19921 actgctgagc aagcgctcat cacgcgcatc gaagccattc gcgccgatct tttccgcgac
    19981 gcaaagccac cccaacgcgc gcgcgcgtgt gtcgagccgg atggccaaat aagcgctcgc
    20041 cggcggataa ccggagggaa gcccggagaa aatatctgca ccgtccggcg cgcgcgcgcg
    20101 ccaaggatgg cccgtcgcca gcacctcggt cagcgggccg gcggtcacca gcggcggcgg
    20161 cggcggtcgc tctccgaagt cgatgccgct gcgcacatgc cctgcaacac cttttccgtg
    20221 tcctgcgccg cggccagttg gatgttgagg tcgacgagcg ccgcaatgcg cgcgttggcg
    20281 gccgcgagcg cattggaggt ctcggtcagt tttcttgtca acaggagtca gtgctcttga
    20341 tcgaattgct cgctcggcga tgccgccgat tcggggacgc cggccagtac ttgttcgact
    20401 gccttgagaa tgacgaccgg ttcgcacggc ttcatgagga cgaagccgac ctggcatgcc
    20461 ctcgccaacg tcttcgtttc ctgctcgttg taattcgcgg tgcaaaaaat aacccgggtg
    20521 gcgctcaact cgggatcttc tcgcagcgct cggaccaatc caaagccgtc gatggtcggc
    20581 atcatgatgt ctgagatgac gacgtccggg cgctcggcgc gcgcgacgat caacccatcg
    20641 aggccgtcgc ttgcctcgat ggtcagatgg ccgtcggaac tcagcagggc caccaactcc
    20701 ttacggtaaa cggaactgtc atccacgacc agaatctttg ccatgttgcc gctagggaag
    20761 aagcactgtc gcctggtatt cgcagaggaa tttcgccaat cggctgcact gcggtccgag
    20821 cgcagatttc accatgtagc ccgcgacatt gtgctgataa gcgcgggctc gatccttgtc
    20881 ggctccagag gtcgtcaaga cgaacacgac cgtggcatgc aattgagggt cgacgcgtag
    20941 ctcctgcaga aattcggtgc cgctcatgcg cggcatattg aggtcgagga gcgccaggta
    21001 aggtttctcg attttctgcg ttgcgtgagt gccgcgcaag atgtccaacg cttcgcggcc
    21061 atcctcggcg acgacgatgg gataggaaat gctgtgcttg cgcaaaccgc gaaccacggc
    21121 ttccgccgcc acgtcatcgt cctcgatcag cagaatactg agttcagggt tactgctcat
    21181 gtc
//
LOCUS       DASWZT010000348         2296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_12530, whole genome shotgun sequence.
ACCESSION   DASWZT010000348 DASWZT010000000
VERSION     DASWZT010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12530"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1375)
                     /locus_tag="VGE92_09665"
     CDS             complement(<1..1375)
                     /locus_tag="VGE92_09665"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEY1314136.1"
                     /translation="MNSQRTIFPAFTALLMFFGASLSGGAAESAHPWSGVGHLSISSS
                     VASVSQNVGVVSIEVTRRGGSSGTASVLCATRNNTAVSGIDYTAVSQTLDWGSGDTSG
                     KFCNVPISDASPFSGQKTFIVQISDASGAALGRVSSATVTINGSGAGTAGTLTLAAPT
                     YAVAQNAGSVTISVNRTGGSSGAATVAYATANGSAVAGTDYTSEQGALSWASGDATPK
                     TFVIPVSNATPFAGTKTLAVAIASAQGAVLGTANTSAIVSINGDAASPPSVSLSASPT
                     SIASGGSSMLTWSASNATACTASGGWSGSMATSGSQSTGALGAAATYMLGCTGSGGTA
                     TQSVTVSISAPTPPTGAVSRPSYNTGNGFFVLNGKLYDANGNEFRFRGVDRAHYDDPA
                     QPGVSNTHANAVRLFVETNYGASVATLVNDVQTQIINYQEVPIVTSPTTTAGTLTSCS
                     NDTSVLNS"
     gene            complement(1567..>2296)
                     /locus_tag="VGE92_09670"
     CDS             complement(1567..>2296)
                     /locus_tag="VGE92_09670"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1314137.1"
                     /translation="RMSFFAVVVISVGAVAALLRLLPRHIAAERGSIVKEFVALRDAQ
                     VLLALLTSVLCSASLFCVFTYIAPLLTDVSGFSQAAIAPILLVFGVGLTVGSTLGGRL
                     GDRHLVPSIFLVMMCTSLVLTAMHWSAAAHAPMVLTLFVWGMLAFSLVPLLQTLIVHH
                     AAAAPRLASTLNQGAFNLGNAIGAWSGSYLLSERLPLADLSWASVGFTLSAIGVTALG
                     NLFFARTATVHTSQRCDAGARAAV"
BASE COUNT          456 a          711 c          710 g          419 t
ORIGIN      
        1 ccgaattgag cacggaggta tcattgctgc agctggttag agtccctgca gtcgtggtcg
       61 gggatgtgac tatcggcact tcttggtaat tgatgatctg ggtctgcaca tcattgacga
      121 gcgttgcaac agatgctccg taattggtct cgacaaagag ccgcaccgca ttagcgtgag
      181 tgttactaac tcccggctgc gccggatcgt cgtagtgcgc ccggtccacg ccgcgaaaac
      241 gaaactcatt gccgttcgca tcgtagagct tgccgttgag cacgaagaag ccgttgccgg
      301 tgttgtagga ggggcgtgaa acggcgcccg tgggcggggt cggagcagat atcgacaccg
      361 tcaccgactg ggttgccgtt ccgccgctac ccgtgcagcc gagcatgtag gtcgccgctg
      421 cgccgagcgc tccggtcgac tgagagccgc tggtggccat ggaaccggac caaccgcccg
      481 aggcggtgca cgccgtcgcg ttgctcgcgg accaggtcag catcgagctg ccgccgctcg
      541 cgatgctggt cggactggcg ctcaaggaca cgctgggagg gcttgccgca tcgccattga
      601 tcgagacgat ggcggaggtg ttggcagtcc ccaggaccgc gccttgcgcg ctggcgatcg
      661 cgaccgccag ggtcttggtt cccgcaaacg gagtcgcgtt gctgaccggg atgacaaagg
      721 tcttcggggt cgcatcgccg ctggcccagc tgagcgctcc ttgctcggag gtgtagtcgg
      781 tgcctgcaac cgcgctgccg ttggccgtcg cgtaagcgac cgtcgcggcg ccactggagc
      841 cgcccgtacg attcacgctg atggtcacgg aacccgcgtt ctgtgcgact gcataggtcg
      901 gcgcagccag agtcagagtc cccgcagtcc cagcgccgct gccgttgatg gtgacggtgg
      961 ccgaactgac tctacccaaa gccgcgccgc tcgcatcgct tatctgcacg ataaaggtct
     1021 tctggccgga gaagggagat gcgtcgctga tcggaacgtt gcagaatttg cccgacgtgt
     1081 cgccgcttcc ccaatcgagc gtttggctta cggccgtgta gtcgatcccg gacaccgcgg
     1141 tgttattgcg agtggcgcaa agcacgcttg ccgtgccgct ggaaccgccc ctgcgagtca
     1201 cctcgatgct cacaactccc acgttctgcg aaacgctcgc gaccgaggaa gaaatcgaca
     1261 gatggcccac tccactccac gggtgtgcgc tctccgctgc gccgccggat agactcgcgc
     1321 cgaaaaacat cagtagcgcg gtaaatgcag gaaatatcgt tcgttgggaa ttcattctat
     1381 tcacctttct gtcgcgatgg ctgtactacg cgcacttgta gcggcaaaga cggcgcatgg
     1441 ccattagtct ccgtatggat tcatgtctcc atatggattc atgtctccat atggattcaa
     1501 gttgcggaaa gataggttta tggaaaattg ggccgcggga gtggccggtc gcgagtgcga
     1561 gacctgctaa acggctgctc gcgcgcctgc atcgcatcgc tgcgaggtat gcacggttgc
     1621 agtccgcgca aagaacagat tgccgagcgc cgtgacgccg atcgcgctca gcgtgaatcc
     1681 gacgctggcc caactgaggt ccgccaatgg cagccgctcg ctcaacaggt aactgccgct
     1741 ccaggcgccg atggcattgc cgagattgaa tgccccttgg ttcagcgtcg aggcgagccg
     1801 cggcgccgcc gcggcgtgat gcacgatgag cgtctgcagc aggggcacca gcgaaaacgc
     1861 caacatcccc cacacgaaca gcgtcaacac catcggagca tgcgccgccg cgctccaatg
     1921 catcgccgtg aggaccaggc tggtgcacat catgaccagg aaaatggatg gcactaggtg
     1981 ccgatcgccg agccttccgc ccaacgtgct cccgaccgtc aaacccaccc caaacaccaa
     2041 caagatgggc gcgatggcgg cttgtgaaaa gccgctgacg tcggtcaaca gtggcgcgat
     2101 gtaagtaaaa acgcagaaca aactcgccga gcacaacacg ctggtcagca gtgccaatag
     2161 cacttgagca tcgcgcagcg cgacgaattc tttgacgata ctaccgcgct cggcggcaat
     2221 gtgccgcggc aagagtctca gcagcgctgc cactgcgccc acactgatga caaccaccgc
     2281 gaagaagctc atacgc
//
LOCUS       DASWZT010000349        19382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_75215, whole genome shotgun sequence.
ACCESSION   DASWZT010000349 DASWZT010000000
VERSION     DASWZT010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 19382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19382
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75215"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VGE92_09675"
     CDS             complement(<1..373)
                     /locus_tag="VGE92_09675"
                     /inference="COORDINATES: protein motif:HMM:NF024750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HEY1314138.1"
                     /translation="MLTKLLIDNTKPGAKSRRLSDGRGMYLEIAPAGGRWWRFKYRLA
                     GKERRISLGVYPDVGLAEARDRREQARKQVAAGIDPAQQRRAEKIALVERTENTFDAI
                     AREWFALFSPKWVSTHSSKVIG"
     gene            complement(1100..1876)
                     /locus_tag="VGE92_09680"
     CDS             complement(1100..1876)
                     /locus_tag="VGE92_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314139.1"
                     /translation="MRAGMYKVIVERPRRGKEGDAFAARLRNDFDGPTRLGMRAGYGC
                     RSLNENLAPLRRYLHAQTGRPWNKVFSEICAGIDRRNTVQQHIHQHIHDFIATDVDVR
                     EGQLVDIAPRSGIFGLGTGISQELYVDPRTGLIRLNSNYHPWRHQLAERRRRDLAEIS
                     MRRRAVDERTLILLLSGVWFCVEVDVLPKEHIVESVIDGKPQRRTTAESSYDVVLRRK
                     ISRAILADRRQCEHLYGSSDLHAVSKRQISKREIRMHRLR"
     gene            complement(2137..2649)
                     /locus_tag="VGE92_09685"
     CDS             complement(2137..2649)
                     /locus_tag="VGE92_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1314140.1"
                     /translation="MSQSLILSPERLHAQHDVSRFENGRHPSLDAWLNNRALASEGLS
                     ARTYVVCPRDSPRRVIGYYAISTAMEQRVSLPSARLRRAMPDQVPMLLIGRLAVDRNY
                     HGKGLGGDLLSDGVRRCLEVSEIAGVRAIITHAIDEDAVHFYQHHGFLRCPLGDRVLL
                     LPIEAARAAI"
     gene            complement(2685..2963)
                     /locus_tag="VGE92_09690"
     CDS             complement(2685..2963)
                     /locus_tag="VGE92_09690"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HEY1314141.1"
                     /translation="MAQPARKDDVIQIRTSAETKAVLNRAAALRGQKLSEFMLDSARR
                     QAEEAILDQRIFILDSKTHDKFLALLDAPTKPSKQLKALLKRKPVWAQ"
     gene            3094..4119
                     /locus_tag="VGE92_09695"
     CDS             3094..4119
                     /locus_tag="VGE92_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY1314142.1"
                     /translation="MSLYRRANSPYWWIRFQLDGREVRASSGTEDREQAEELERHARD
                     SVWRQVKLGEKPPYSWSEARKRWLHETRKRTKAKDEAILSWLDQHLKRRTVQDITRPV
                     VEHLRALKAEETSKSTADRYMALLRAVLRKCVNDWQVLASAPKVSMYRQRSPEARWLT
                     EPEFERLCDKLPEHLALAARFAVTTGLRMRSMTALTWDRVDMKNRRLWIPGEQMKTGI
                     PHGIPLSRKAIKLLRQLKKLNPEGDRVFQWNGKPIDDCNTRAFQDAVTAANLAPLRWH
                     DLRHTFASWAVQNGVTLPELMQIGGWKSYSMVQKYAHLAPEHLAAAAEKASGGRHKNR
                     HTRNKRT"
     gene            complement(4165..4241)
                     /locus_tag="VGE92_09700"
     tRNA            complement(4165..4241)
                     /locus_tag="VGE92_09700"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4205..4207),aa:Met,seq:cat)
     gene            complement(4333..5424)
                     /gene="ychF"
                     /locus_tag="VGE92_09705"
     CDS             complement(4333..5424)
                     /gene="ychF"
                     /locus_tag="VGE92_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEY1314143.1"
                     /translation="MPIRCGIVGLPNVGKSTLFNALTRAQIAAENYPFCTIDPNVGVV
                     PVPDPRLEKLAAIVHPEKILPTTVEFVDIAGLVAGASKGEGLGNKFLAHIREVDAIAH
                     VVRCFENDDIIHVAGKIDPANDIEVINTELALADLDSVERAYQKSVKAAKTADKDAVK
                     TRDVLERVRKQLNQAKPVRTLGLDAHERALLRDMHLLTDKPIMYVANVDENGFTDNPR
                     LDRVRELAKAEGAVVVPVCAAIEAEISQLDEADRGEFLKELKLDEPGLNRVIRGGYAL
                     LGLQTYFTAGPKEVRAWTVHRGATAPQAAGVIHTDFEHGFIRAETIAYDDYIAFKGEA
                     GAKEHGKLRLEGKEYIVKEGDVMHFRFNV"
     gene            complement(5424..6014)
                     /gene="pth"
                     /locus_tag="VGE92_09710"
     CDS             complement(5424..6014)
                     /gene="pth"
                     /locus_tag="VGE92_09710"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY1314144.1"
                     /translation="MTGLPLRIIVGLGNPGPEHQVTRHNAGFWFVDLLARRHGGEFRD
                     YRKYSGETAKINVSGQDIILLKPTTYMNRSGLAVRQLSDFYKIAPQDILIAHDELDLP
                     VGSVRLKQGGGHGGHNGLRDAIAHIGETFWRMRLGIGHPGKKTEVIDYVLTRAPGPEE
                     DLILDAVGTAADCMPLLLEQGAERAMTRLHSRAGKV"
     gene            complement(6094..6840)
                     /locus_tag="VGE92_09715"
     CDS             complement(6094..6840)
                     /locus_tag="VGE92_09715"
                     /inference="COORDINATES: protein motif:HMM:NF004128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEY1314145.1"
                     /translation="MKTSFELAAEFRETQGKGASRRLRHEGKVPAILYGGHSDARALT
                     LSHQKLLIMLDNERFYSTILNLRVGDQTQAAILKDVQRHPFKNAIVHVDFQRVEENEK
                     IRINIPLHFTGAAVSPGVKSQGGIVSHMRTETEVSCLPKDLPEFIEVDVSGLSLNESI
                     HLSQLKVPEGVTLVELAKDDAAVVAIHSPRAEEPEPTAVAAAGAAPAAEGAAAAAAAP
                     AAAGADAAKKAEPAKKEEAKKEPAKKDAKK"
     gene            complement(6910..7878)
                     /locus_tag="VGE92_09720"
     CDS             complement(6910..7878)
                     /locus_tag="VGE92_09720"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEY1314146.1"
                     /translation="MSQSKEFDTLTIAVFSGKANPTLAHDIARHLHVQLGRADVGRFS
                     DGEVSVELMENVRGRDVFIVQSTNPPAENLMELLMMTDACRRASAKRITAVVPYFGYG
                     RQDRRPRATRVAITAKLVANMIASAGVDRLLTVDLHADQIQGFFDIPVDNVYASPVLL
                     GDAWKQKYQNMVVVSPDVGGVVRARALAKRLDDADLAIIDKRRPRPNESKVMNIIGEV
                     EGRTCVLIDDMVDTAGTLCQAARALKDEGAAKVVAYITHPVLSGNAIERISKSVMDEL
                     VVTDTIPLSDAGKACKQIRQLSVAALLAETVRRINDEESVSSLYID"
     gene            complement(7898..7972)
                     /locus_tag="VGE92_09725"
     tRNA            complement(7898..7972)
                     /locus_tag="VGE92_09725"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7938..7940),aa:Gln,seq:ttg)
     gene            complement(8013..8873)
                     /gene="ispE"
                     /locus_tag="VGE92_09730"
     CDS             complement(8013..8873)
                     /gene="ispE"
                     /locus_tag="VGE92_09730"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEY1314147.1"
                     /translation="MPPHPRAWPAPAKLNLFLHIVGRRPDGYHELQSCFQFVDLCDEI
                     TLEVRDDGRIRRLVEIDAVAEDADLCVRAARALKTAASCALGADISVLKRIPLGGGLG
                     GGSSDAATCLVALNHLWKLHWPVDDLAALGLKLGADVPVFIRGRVAWAEGIGERLTAL
                     YPPFAPPETNYLILKPKIAVSTAQVFQDIDLTRNSAPITIHGFLGSGGRNDCVGVVRR
                     RYPEVARALDWLSAFGPARLTGTGACVFLAVETLARGRKILGKLPPEFDAFLARGLND
                     SPLLERLATG"
     gene            complement(8873..9448)
                     /gene="lolB"
                     /locus_tag="VGE92_09735"
     CDS             complement(8873..9448)
                     /gene="lolB"
                     /locus_tag="VGE92_09735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HEY1314148.1"
                     /translation="MRRIWLVLISCAVLAACVTAQRAPAALSAWEQRAADLQKLGSWR
                     LDGRAAVAVGTQGWQATLNWQQRSDLTEVHLSGPFGVGALLLTRTPAGLSLNGAAPSA
                     DVVAQLQERLGFELPMDHLRFWLLGVPDPSAAFELKRNGQDRASQVIQDAWTVDYDRY
                     MPVAGDLLPAHVVLSREGVRVRIAVDHWELP"
     gene            complement(9453..11195)
                     /locus_tag="VGE92_09740"
     CDS             complement(9453..11195)
                     /locus_tag="VGE92_09740"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1314149.1"
                     /translation="MVSISILARASLALGLLACTACIGVGPKSQDTAAPRTFYTVTAE
                     IALARHEPRVAALQYAAAAAADPDVGLLERATQVAADCLQPSVVENITARWIGIDPKS
                     VEAHRAAGQAALALDDIDQAAAHYRLVLTSSPLGTDKEFAALETYLAGDDNIFGSRQL
                     ANRLATWFPGSAPALRVQGFAALRADDPAAAVQSFTALLALPADSAAAGDETARRELR
                     QTLARARIMAGDADAPLAQARDTVTRDNTPANRLDYALLLMTAQREQAAAQQLEILSH
                     NAEYGPVALRLLGLIEFQEGHWDAASAKFAELLRAEKYLDDAFYYLGLIADHHDDPEH
                     ALRLFAQVQSGDNSVPALLRATTILQTHGATAAADELLDRLVEDEPQRAPEILTARAR
                     MYADAGNYPRAMTVLEHGELVYPDSVDLRYAKATLYEEEGRVSDALRELSAVLKSRPD
                     DPSAMNALGYTLADHSRNLKRARKLIESAHAAAPTNAAILDSLGWVLFRQGQSAQAEP
                     YLRTAYADDRGGEIAAHLGEVLWQLGRQADAEHIWTEAGATDGENKLLKSTRERFHVA
                     PPPPAAPAALPQLN"
     gene            11300..12556
                     /gene="hemA"
                     /locus_tag="VGE92_09745"
     CDS             11300..12556
                     /gene="hemA"
                     /locus_tag="VGE92_09745"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEY1314150.1"
                     /translation="MAFKILGINHKTAPVALREKVAFSEERLLTALQTLRQEAGVAEV
                     VILSTCNRTEVYWAGSASGEELSLWLERHHGNNLDLAASLYVHQEQRAVEHAFSVASG
                     LDSMVLGEAQILGQLKDAYRVAQAAGSTGPSLNKLFQAAFSAAKRVRSETRIGENAVS
                     IASASVSLARRVYSDLSARTALLVGAGEMNALVARHFASAGVQRMVIANRTRARAEAL
                     AAELKAHAVGLDELDTELVQADIVIACTASAVPLIGKRAAESAIRARRRRPIFMVDLG
                     VPRDIDPAIADLEDVYLFSIDDLQQLVEENRQQREVAAGGARLVIDEEVARFLSESRA
                     QDAGPAIRALRQQAEGIRSQSLEQARRMLAAGKTTDEVIEYLANTLTNRLLHTPTQAL
                     RQAAELEDSSLAQALTRLLISYNPPP"
     gene            12553..13635
                     /gene="prfA"
                     /locus_tag="VGE92_09750"
     CDS             12553..13635
                     /gene="prfA"
                     /locus_tag="VGE92_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEY1314151.1"
                     /translation="MKDSIRRKLEKLDERFEEISRLLSDAGVIARQNQFRELSMEYAK
                     LGPLVERYRRFIALEADLAIAQEMASDKDAATQAMGEEELAELQPRLAAEELELQRLL
                     VPKDPQDDSNIFLEIRAGTGGDEAAIFAGDLFRMYARYAESKGWEVETLSESPGEHGG
                     YKEVISRIIGRGAYSLLKFESGTHRVQRVPATEAQGRIHTSACTVAILPELAEVEAIE
                     LNPSDLRIDTFRASGAGGQHVNKTDSAIRITHIPTGVVVECQDERSQHKNRSRAMSLL
                     KSRLLAAEREKQQSQQAQSRKLQVGSGDRSERIRTYNFPQGRVTDHRINLTLYKLDEV
                     MNGHLEELIRALNQEHQAEELAHQVE"
     gene            13632..14474
                     /gene="prmC"
                     /locus_tag="VGE92_09755"
     CDS             13632..14474
                     /gene="prmC"
                     /locus_tag="VGE92_09755"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY1314152.1"
                     /translation="MNTVAESISRAARSLSSHSESPRLDAELLLGKILGLSRAGLIAR
                     SNEPVSGESQKSLANLLEERRRGTPVAYLTGTREFWSLALHVTPAVLVPRPETEILVD
                     LALQRLPEDQAATVLDLGTGSGAIALAIAAERPLSQVTGVDISAAALEIARQNASALH
                     LTRIDWRLGSWFAPVVKQRFDMIVANPPYIAAADPALERLAAEPAIALCDGPTGLEAL
                     STIAAGAASHLTRHGWLLLEHGSTQGPEVARLLHSHGFSSVRSHPDFSGKLRVTLGTL
                     HSPP"
     gene            14483..14971
                     /locus_tag="VGE92_09760"
     CDS             14483..14971
                     /locus_tag="VGE92_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY1314153.1"
                     /translation="MIRFETNLGDFTVELFEQDAPLSVANFLRYIDDGFFDGTIFHRI
                     VPGFVIQGGGFTEDMTQKKTHAPVKNEADNGRKNARGSLSMARTNDINSATSQFFVNL
                     KDNDFLDHSRGNFGYAVFARVTDGMDVIDKIAAVETGRKRGFDDVPVQAVIMKSVRRI
                     PA"
     gene            14980..15717
                     /gene="mtgA"
                     /locus_tag="VGE92_09765"
     CDS             14980..15717
                     /gene="mtgA"
                     /locus_tag="VGE92_09765"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEY1314154.1"
                     /translation="MARDRSIFRRLLRAIAWLIGLGLLASVAAVAALRWIDPPFTAFM
                     AEAQAEAWAHRDSSYVSRHSWVDLQRISPNLPLAVVASEDQKFPEHWGFDVEAIEKAY
                     ALNQHSHKVRGASTISQQVAKNVFLWSGRSYFRKGLEAYFTVLIESLWPKRRILEIYL
                     NIAEFGPGTYGAEAAAQRFFHKTAAQLTRGDAALLAAVLPNPERYSVSAPSRFVQQRR
                     EWILGQMQALGGPEMLDEIDAYPNRRR"
     gene            complement(15696..16910)
                     /locus_tag="VGE92_09770"
     CDS             complement(15696..16910)
                     /locus_tag="VGE92_09770"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY1314155.1"
                     /translation="MKSIDLVYFNAGGGHRSAAVALEMAIRDLALPWHVRLVNLFEVL
                     DPKDVFAKTTGMKPEDYYNARLARGWTLGLTQELKVFQALIRLGHRTLTARLRRHWQR
                     TLPDLVVSLVPNFNRAMYQALTAARPNAPFVTILTDFADSPPHFWIEPHQAQHLICGT
                     PKAVLQARAAGYDDTRIHATSGMIIRPDFYRNLRIERRAERAKLKLDPDRPTGMVMFG
                     GHGSKAMRRIARRLDDTQLILICGHNCELADDLRAMNSAAPRAVIGFTAQIGLFMQLS
                     DFFIGKPGPGSISEAIQQQLPVIIVRNAATMPQERYNTHWVEENGAGLVLDSFRTIRA
                     AVATLTSGMNEYRGRVARIRNRAIFEIPPILQRILEREQAQTSSRPARQIAAGVDSTV
                     CRELCDQRRRFG"
     gene            16990..18423
                     /locus_tag="VGE92_09775"
     CDS             16990..18423
                     /locus_tag="VGE92_09775"
                     /inference="COORDINATES: protein motif:HMM:NF017835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcvB/VirJ family lysyl-phosphatidylglycerol
                     hydrolase"
                     /protein_id="HEY1314156.1"
                     /translation="MVVSCAARAGWLLAASLLLAPTADFAAEPTPAAPTQLSHGRFKD
                     FSVFQPKGVPTSFVLFLSGNEGWNAAADTLARQLVQHGAMVTAIDWIKLKAAFEADGD
                     QCVFPDGDLENLSHFVQAYFHTPTYLSPLLVGVSSGAAMSYAMLAQAPRNTFAAALTL
                     GFCPNLDLEKPLCKGSGVEFTRSEHLRGVELLPNKSLGNPWLDLQGADNRNCPVATVR
                     NFISQARGAALVTLPQASHDFSSLASWLPPFTAAYDRLAAHNPTRQAAPPPAGLSDLP
                     LVEVPVRPGAPAADSFAIIMSGDGGWAGLDQDVAAALTAQGIPVVGLDSLRYYWTART
                     PQGLAADTDRIIRYYLAHFGKQRVLLIGYSQGADVLPFAVNRLPPATRARVALAAVMG
                     MSEHALFEFHLSSWISDSDSGPKTLPEIDRIAGVPVLCIYGADEHDSLCPQLDPQRFK
                     VVKLKGGHHFDGDYANLALQILAAAHP"
     gene            complement(18432..>19382)
                     /locus_tag="VGE92_09780"
     CDS             complement(18432..>19382)
                     /locus_tag="VGE92_09780"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEY1314157.1"
                     /translation="FDDYPLAELLPYIDWMPFFNAWEFAGKFPDILQDPVVGAAASAL
                     YADATRMLQAVVQERWLKARAVVGFFPANGSDDDIAVFSDETRHTVRVRLHHLRQQKS
                     KPQNQAQSCLADFIAPIGSGRSDYVGAFAVTAGIGIDAHVARFEAAHDDYSSIMLKAL
                     ADRLAEALAERMHERVRRELWGYAPDERLDGEGLIREQYQGIRPAPGYPACPEHTEKA
                     TLWQLLDPEQNAGIRLTESFAMYPTAAVSGFYFSHPQAHYFGVGQIDHDQVASYARRK
                     KWTLAEAERWLAPNLGYEPEASETLPGEAAATDAATADAA"
BASE COUNT         3709 a         6272 c         5772 g         3629 t
ORIGIN      
        1 gaccgataac tttgctcgaa tgagtcgaaa cccatttcgg cgaaaacaag gcaaaccact
       61 cacgcgcaat cgcgtcgaat gtgttttcag ttcgctcaac caaagcgatt ttttccgctc
      121 ggcgctgttg agcgggatca atgccagcgg caacctgttt tcgagcctgt tcccgtcggt
      181 cgcgcgcttc ggccaagccc acgtcgggat acacccccaa cgaaatgcgt cgctctttgc
      241 cggctaggcg gtacttgaag cgccaccagc gtccgccagc aggcgcgatt tctaggtaca
      301 ttccgcgacc atcagagagc ctccgcgact tcgcacctgg cttcgtgttg tcgatcaata
      361 acttagttaa cacttggggg caactccgtt ttgcgaacac tcgtccgtgg gggcaactcg
      421 atgctcgaga gatactcttg ggggcaacaa tcggttaagt tgcccccaat acaggcacgc
      481 tgtcaagcag cgcgaaatgg gcggcgcgcg gggccgatgc tactaattat cgttatttta
      541 cagagacttg ggcgatgtca ggagtagtca ggagaggtgg agcgaggcga ggcggggcgg
      601 catttggtgg tgagagaggg actcgaaccc tcggcctcgg cattatgagc gcagaagttt
      661 agaaacgcgt ctagtcattt caacaacttg ccgcgtggcg cccgttgaaa ttagcaccat
      721 agagcacagt aaagcacaac agagtcacgc aaaactcacg cacgatttga cgccggacgc
      781 gactttgcag ctggccgttc cattgcatca tcgccgacct ccaagcgaaa aaggaccgga
      841 cgatcgcgcc gacccttccc tagcgctacg aaactgagca ttgtgaatcc cactatgacg
      901 cttttgggag ccgggggctg tggacttccg cgtaaaagct ctcgagtagg ccggtagtac
      961 tcaggtgcgc actttttccc attccagcta cggcgaaatc attttaacac ggagagccga
     1021 cgcgggccta cctgatcgtg aattcaggag atcccgtgcg cgcgggtcgc cagtcgaccg
     1081 cacggtacat ccctattttc tagcggaggc gatgcattct tatttctcgt ttggaaattt
     1141 gccgcttgct cacagcatgg agatcgctcg atccgtacag gtgctcgcat tgacgccgat
     1201 ccgccaatat cgcccgtgag atttttcggc gcagcacgac gtcatacgac gattcggccg
     1261 ttgtacgcct ctgcggcttg ccatcgataa cactctcaac gatgtgctcc ttcggcagca
     1321 cgtcaacctc gacacaaaac cacacaccac ttagcagtag aataagagtc cgctcatcca
     1381 cggcgcgacg ccgcatcgag atctccgcga gatcacgcct gcgacgctcg gcgagttggt
     1441 ggcgccaggg gtggtaattg ctgttgagac ggatcaaccc cgtacgtgga tcgacataca
     1501 gttcctgtga tattccggtg ccaagcccaa aaattcccga acgaggcgcg atgtccacca
     1561 gctgcccttc gcgaacgtcg acatcggttg ctatgaagtc gtgtatatgt tgatggatgt
     1621 gctgctgcac ggtgttccgc cgatcgatac ccgcacaaat ctcgctgaac accttgttcc
     1681 aggggcgtcc ggtctgcgca tgcaggtacc ggcgcagcgg agccaggttc tcattgaggc
     1741 tacgacagcc atagcccgct cgcatgccga gccgcgtcgg tccatcgaaa tcgttacgga
     1801 gccgggcggc gaacgcatcc ccctccttcc cgcggcgtgg ccgctcgaca attaccttat
     1861 acatgcctgc gcgcatgggt ggcccccgat aacaaaaagc cctcggcaag aatccctgcc
     1921 gggggcttaa agctcgcgcg acgcgatgat cggcccaatc ggcttcgggt aaatcgctac
     1981 ccgcaaccgt gtgagctcgc gggctggcgc tacattgagt gcaagttcgt cgtcttcgat
     2041 aaaattcgca tgtagtgctc caaattcaga gccgcgcagg atacatcccc aagagccagt
     2101 gtcaagagtt ggcggacgag tgacccactc caaaactcat atagccgccc tcgcagcctc
     2161 gatcggtaag agcaggactc gatcaccgag cggacagcga agaaaaccat gatgctgata
     2221 aaagtgaact gcatcttcat cgatggcgtg ggtgatgatc gcgcggacac ccgcgatttc
     2281 cgacacctca aggcatcgtc taacgccgtc ggacagcaga tctcccccga gtcctttgcc
     2341 atggtagttt cgatcgacgg ccaaccgacc gatgagcagc ataggcacct gatcgggcat
     2401 ggcccgccgg agcctggcag agggcaacga aacgcgctgt tccatggctg tcgatatcgc
     2461 gtaatagcca atgacacgtc gcggggagtc gcgtggacac acgacatagg tacgggcgga
     2521 cagcccttcg ctcgcaagcg ccctattatt tagccaggca tccagcgaag ggtgtcttcc
     2581 attctcgaaa cgcgagacat cgtgttgcgc gtgcaaccgc tcgggcgaca ggatcagact
     2641 ctgactcaca cgcagcgctt cgtcaaaatg tcaacggctc gcagtcattg cgcccaaacc
     2701 ggcttgcgct taagcaacgc cttcagctgc ttactcggct tggtaggcgc gtcgagtaag
     2761 gcaagaaact tgtcgtgcgt ctttgaatcc aatatgaata tcctctgatc caagattgcc
     2821 tcttccgcct gacggcgcgc gctgtccagc atgaattccg agagcttctg tccgcgcaaa
     2881 gctgccgcac gattgaggac cgccttagtc tcagcggatg tccgaatctg aataacatcg
     2941 tcttttcttg ccggttgcgc cataggatct cctgattacc taacttacat tattgtaccg
     3001 acattgtcaa tacacggtga ctcgagcctt gtctgagggc atcatatctc gggcctgttc
     3061 aagccctcgg actttgtatc ccaagaatcc cccatgtccc tctaccgccg cgccaactcg
     3121 ccctactggt ggatccgttt tcaactcgac ggccgtgaag tgcgcgcctc aagcggtacc
     3181 gaggatcgcg agcaagccga ggaactcgag cgacatgcac gcgatagcgt gtggcgccaa
     3241 gtcaaattag gtgagaaacc tccctactca tggtctgagg ctcgcaagcg ttggctccac
     3301 gaaacacgta aaagaaccaa agccaaagac gaagcaatcc tgtcttggct cgaccagcac
     3361 ctcaagcgcc gaacggtgca ggatattacg cgccccgtag tcgagcatct gcgcgcgctg
     3421 aaggccgaag agacctcaaa atcgacagcc gaccgctaca tggcgttgct gcgcgctgtc
     3481 ctgcgcaagt gcgtcaacga ctggcaagtg ctcgcatcgg cgccgaaggt atcgatgtat
     3541 cgacaaagat caccggaagc ccgctggttg accgaacccg aatttgaaag gctctgcgat
     3601 aaattacccg agcatttggc gctcgccgcg cgattcgcgg tgaccacagg tcttcgaatg
     3661 cgatccatga cggcactcac gtgggatcga gtcgatatga agaatcgccg gctctggata
     3721 cccggcgaac agatgaagac aggaatccca catggcatcc cgctttcccg caaagcgatc
     3781 aagctcctgc gacagctcaa gaaactcaat cccgaaggcg atcgggtctt tcaatggaac
     3841 ggtaaaccga tcgatgactg caacacgcgc gcgtttcaag acgctgtgac ggcggcgaac
     3901 ctcgcgccgc tgcgttggca tgacctgcgc cacacgttcg cctcctgggc cgttcagaac
     3961 ggcgtgacgc taccggaact catgcaaatc ggtggctgga agagctacag catggtgcag
     4021 aaatacgcgc acctggcgcc ggagcatctc gccgctgccg cggagaaggc cagcggtggg
     4081 cggcacaaaa accggcacac tagaaataag cgaacctaat cgtagagaag atcttgcgaa
     4141 aaaggcctat ttatcaggca tatttggtgg tgagagaggg actcgaaccc tcgacctcgg
     4201 cattatgagt gccacgctct aaccagctga gctacctcac catcttttaa ctcttgacct
     4261 cgctaggctc tgcctgaaga gccgcggatt ctccgcagcg ccgactaccc tgtcaaggcg
     4321 ccccatccgc tcctacacgt tgaacctaaa gtgcatgaca tcgccctctt tgacaatgta
     4381 ttccttgccc tccaggcgca gcttcccatg ctccttggcg cccgcctcgc ctttgaaagc
     4441 gatgtagtcg tcatacgcga tcgtttccgc gcggatgaag ccatgctcga aatccgtgtg
     4501 gatcacgccg gccgcctgcg gcgccgtcgc gccgcgatgc acggtccatg cgcgcacctc
     4561 cttcgggccg gccgtaaagt acgtctgcaa tccgagcagc gcatacccgc cgcgaatcac
     4621 gcgattgagt ccgggctcgt cgagcttcaa ttccttgagg aattccccgc gatccgcctc
     4681 atcgagctgc gatatctccg cctcgatcgc cgcgcacacc ggcaccacca ctgcaccctc
     4741 cgccttcgcg agttcgcgga cgcggtccag ccgaggattg tcggtgaatc cgttctcatc
     4801 gacattggcc acatacatga tgggtttatc ggtgagcagg tgcatgtcac gcagcaatgc
     4861 ccgctcatgg gcatccaatc ccagcgtgcg caccggcttc gcttgattca gctgtttgcg
     4921 cacccgctcg aggacatcgc gcgttttgac cgcgtccttg tcggcggtct tcgccgcctt
     4981 gacggatttc tgatacgcgc gctcgacgct gtcgaggtcc gcgagcgcga gttccgtgtt
     5041 gatgacttcg atatcgttcg ccgggtcgat ctttccggcc acatgaataa tgtcgtcgtt
     5101 ctcgaagcag cgcaccacat gcgcgatcgc atccacttcg cgaatgtgcg cgagaaactt
     5161 gttgccgagc ccctcgcctt tcgatgcgcc ggcgaccaag cccgcgatat cgacgaattc
     5221 caccgtggtc ggcaggatct tctccggatg cacgatcgcc gccaacttct ccaggcgcgg
     5281 atccggcacc ggcaccacgc cgacgtttgg atcgatggtg caaaacggat aattttccgc
     5341 cgcgatttgc gcgcgcgtca gcgcgttgaa caacgtcgac ttgccgacat tgggtaaccc
     5401 cacgatcccg cagcgaatcg gcattagacc ttccccgcgc ggctgtgcag ccgcgtcatg
     5461 gcacgttccg cgccttgctc gagcagcagc ggcatgcaat ccgccgcggt gccgaccgca
     5521 tcgaggatca ggtcctcttc ggggcccggc gcgcgcgtta aaacatagtc gatgacctcg
     5581 gtcttttttc ccgggtggcc tatccctaag cgcatgcgcc aaaatgtctc gccgatgtgc
     5641 gcgatcgcat cgcgcaggcc attgtgaccg ccgtggccgc cgccctgttt caggcgcacg
     5701 ctacccaccg gcaaatccag ctcgtcgtgc gcaatcaaga tatcttgcgg agcgatcttg
     5761 tagaaatcgc tcagctggcg cacggcgaga ccgctgcggt tcatgtaagt cgtgggcttc
     5821 aatagaataa tgtcctgtcc gctcacattg atttttgcgg tttccccgga atacttgcgg
     5881 taatcgcgaa attcgccgcc gtgacgccgc gcaagcaagt ccacgaacca gaaacccgcg
     5941 ttgtgacgcg tgacctgatg ttcaggaccc gggttgccca ggccgacgat aatgcgcagc
     6001 ggtaaaccag tcatgatcgc gctcatcaaa ggctaaattt gaaacagcaa agggccgcct
     6061 cgggacggcc ccgctgatcc atttccgatg acgctacttc ttggcgtctt tctttgccgg
     6121 ttcctttttg gcttcctctt tcttggcagg ctccgccttc ttcgccgcgt ctgcgccggc
     6181 cgccgcgggt gccgccgcag ctgctgccgc gccctccgct gccggtgctg cgcccgccgc
     6241 cgccaccgcg gtgggttcgg gctcttcggc acgcgggctg tggatcgcca cgaccgctgc
     6301 gtcgtctttc gcgagttcca ccagcgtcac gccctcgggg accttcaact gcgacaaatg
     6361 gatgctctcg ttgagcgaca gtccggagac atccacttcg atgaattccg gcaaatcctt
     6421 cggcaggcag gacacctccg tctcggtgcg catgtgcgac acgatgccgc cctgggattt
     6481 gacgcccggg gagacggccg cgccggtgaa atgcagcgga atgttgatgc ggatcttctc
     6541 gttctcctcg actcgttgga agtcgacgtg cacgattgcg ttcttgaacg ggtgccgttg
     6601 cacgtccttc aagatcgccg cctgcgtctg gtcgcccacc cgcagattga ggatggtcga
     6661 gtaaaaacgc tcattgtcga gcatgatcaa gagcttctga tggctcaagg tgagggcgcg
     6721 cgcgtccgag tgcccgccat agaggattgc cggcactttt ccttcgtgac gcaggcggcg
     6781 gctcgcacct ttgccttgcg tctcgcggaa ctcggcggcg agttcaaaag aggttttcat
     6841 aatcttctcc agttacatca caaacacggc gcccgcgcat cgcgaccaat tgcacggaca
     6901 ccgaccaatt caatctatgt acagcgaact caccgattcc tcatcgttga tacggcgcac
     6961 ggtttccgcc agcaacgccg ccacgctcag ctggcgaatc tgtttgcagg ccttaccggc
     7021 gtcggacaga ggaatcgtgt ccgtcaccac caattcatcc attaccgatt tcgaaatgcg
     7081 ttcgatggcg ttgccggaca gcaccgggtg cgtgatgtag gcgaccacct tggccgcacc
     7141 ctcgtctttc agcgcgcgcg ccgcctggca caacgtgccc gcggtatcca ccatgtcatc
     7201 gatcagcacg caagtacgtc cctcgacctc gccgatgatg ttcatcacct tcgactcgtt
     7261 gggccgcggc cggcgtttgt cgatgatcgc caaatccgcg tcgtccaagc gttttgcgag
     7321 tgcgcgcgcg cgcaccacgc cgcccacatc cggcgacacc accaccatgt tctgatactt
     7381 ctgcttccac gcatcgccca acagaaccgg cgatgcatag acgttgtcga ccggtatgtc
     7441 gaaaaacccc tgaatctgat ccgcgtgcaa atcgaccgtc agcaggcgat cgacgccggc
     7501 gctcgcaatc atgttcgcca ccagcttcgc cgtgatcgcc acccgcgtcg cgcgcgggcg
     7561 gcgatcctgg cgcccataac cgaagtacgg caccaccgcc gtgatgcgct tcgccgacgc
     7621 acgccggcag gcatcggtca tcatcagcag ttccatcaga ttctcggccg gcggattcgt
     7681 cgactgcacg atgaacacgt cgcgtccgcg cacattctcc atcaactcga cgctcacctc
     7741 gccatcgctg aatcggccca catccgcccg gcccaattgc acgtgcaagt gccgcgcgat
     7801 gtcgtgcgcg agcgtcggat tcgccttgcc gctgaacacg gcaatcgtca atgtgtcgaa
     7861 ctctttactc tgcgacatgg gcctaaaagc tctaaattgg ctggggtgga aggattcgaa
     7921 cctccgtatg gcgggatcaa aacccgctgc cttaccgctt ggcgacaccc caacttgaaa
     7981 ccaacaatcc gcgaaactcg cgctgccgat tctcagccgg tcgctagtct ttcaagcaag
     8041 ggcgagtcgt tcagtccgcg agccaaaaac gcatcgaact caggcggcaa cttacctaaa
     8101 attttgcggc cgcgagccaa cgtctcgacg gccaaaaaaa cacaggcccc ggtgcccgtc
     8161 aggcgtgcgg gtccaaaggc cgacaaccaa tcgagcgcgc gcgccacctc gggatatcgg
     8221 cgccgcacca cgcccacgca gtcgttccgt ccaccagacc ctaggaaacc gtgaattgtg
     8281 atgggcgcag aattccttgt caaatcaata tcctgaaaca cctgggctgt gctcacagca
     8341 atttttggct ttaaaatcag atagttagtc tcaggcggcg caaacggcgg gtagagtgcg
     8401 gtgaggcgtt cgccgattcc ttccgcccac gccacgcgtc cgcggatgaa caccggcacg
     8461 tccgcgccga gcttgagtcc cagtgccgcg agatcatcga ccggccaatg caatttccac
     8521 aagtgattga gcgccaccag gcaggtcgcc gcatccgaac ttccgccgcc caaaccgccg
     8581 ccgagcggga tgcgcttgag cacgctgatg tcggcgccga gcgcgcacga ggctgccgtt
     8641 ttgagcgcgc gtgcggcgcg tacgcaaaga tccgcgtcct cggcgaccgc gtcgatctcg
     8701 accagacggc ggatccgccc atcgtcgcga acctcgagcg tgatttcatc gcacagatcg
     8761 acgaattgaa agcaggattg caactcgtga tagccgtcgg ggcgccggcc gacgatgtgt
     8821 aagaagagat tgagttttgc cggcgccggc cacgctcttg ggtgcggcgg catcacggca
     8881 gctcccaatg atcgaccgca atgcgcacgc gcactccctc gcggctcaac accacatgcg
     8941 ccggcaacaa atcgccggca accggcatat agcgatcgta gtccaccgtc cacgcatctt
     9001 gaatcacttg cgaggctcga tcctggccgt tgcgcttgag ctcgaaggcg gcgcttggat
     9061 ccggtacgcc gagcagccag aagcgcaggt gatccatcgg caactcgaac cccaggcgct
     9121 cttgaagctg cgccacgaca tcggcgcttg gcgctgcgcc gttcaaggaa aggccggcgg
     9181 gcgtgcgggt gagcagcagc gcgccgacgc cgaacggacc cgacagatgc acttccgtca
     9241 agtcgctgcg ctgttgccaa ttcaaggtgg cctgccagcc ttgcgtgccg acggcgacgg
     9301 ccgcgcggcc gtcgagccgc cagctaccga gtttttgcag gtcggcggcg cgctgctccc
     9361 atgcggacag cgcagccggg gcgcgctgcg cggtcacgca cgccgcgagc acggcacatg
     9421 aaatcaacac cagccaaatg cgtcgcatcg gcttaattca gttgcgggag cgcggcgggt
     9481 gcggcgggcg gcggcggcgc gacgtgaaat cgttcgcgcg tcgacttgag caatttattc
     9541 tcgccatcgg tcgcgccggc ttcggtccag atgtgttcgg catcggcctg ccggccaagc
     9601 tgccataaca cctcgcccaa atgcgcggcg atctcaccgc cgcgatcatc ggcataggcg
     9661 gttctcaagt acggctccgc ctgagcgctt tgaccttgac ggaacagcac ccatcccaaa
     9721 ctatcgagga tcgccgcatt cgtcggcgcc gccgcgtgcg cgctttcgat cagcttgcgg
     9781 gcgcgcttca agttgcgcga atgatcggcc aacgtatagc ccagggcatt catggcggac
     9841 ggatcgtcgg ggcgggattt caagacggcg gagagctccc gcaatgcatc ggacactcgt
     9901 ccctcctctt catagagggt cgccttcgca tagcgcagat cgacgctgtc gggatagacg
     9961 agctcgccgt gttcgagcac cgtcattgcg cgcggatagt tgcccgcgtc ggcatacatg
    10021 cgggcgcgcg ccgtgagaat ctccggggcg cgctgcggtt catcttcgac gagccggtcg
    10081 agcagttcat cggcggccgc cgtcgctcca tgcgtttgca agatcgtggt ggcgcgcagc
    10141 agcgccggca ccgaattatc gccgctctgc acctgcgcaa acagccgcaa cgcatgttcg
    10201 gggtcatcgt gatggtcggc gatcaacccc aaataataga acgcgtcgtc gagatatttc
    10261 tcggcacgca gcaactcggc gaatttcgcg ctcgcggcgt cccagtggcc ctcctgaaac
    10321 tcgatcagcc cgagcagacg cagcgccacc ggaccatact cggcattgtg cgacaggatc
    10381 tccaactgct gcgccgccgc ttgctcgcgc tgcgcggtca tcagcaacag ggcgtaatcg
    10441 agtcgattgg cgggcgtgtt gtcgcgcgtc acggtgtcgc gcgcctgcgc caagggagcg
    10501 tcggcatcgc ccgccatgat tcgggcgcgc gcgagcgtct gccgcaactc gcggcgcgcc
    10561 gtctcatcgc ccgccgcggc gctgtcggcc ggcagcgcca gcaacgccgt gaagctttgt
    10621 accgccgccg ccggatcgtc cgcgcgcagc gccgcgaagc cctggactcg caacgcgggc
    10681 gcggaacccg gaaaccaggt cgcgagacga ttcgccaact ggcgcgagcc gaaaatattg
    10741 tcatcgcccg cgaggtaggt ctcgagcgcg gcgaactcct tgtccgtgcc gagcggcgaa
    10801 ctcgtcagca cgaggcgata gtgcgccgcc gcctggtcga tgtcgtcgag cgcgagcgcc
    10861 gcttgtcccg ccgcacgatg cgcctccacc gacttcggat cgatgccaat ccagcgcgcg
    10921 gtgatgttct cgaccaccga cggctgcagg cagtcggccg cgacctgcgt cgcccgctcg
    10981 agcaacccca catccggatc tgccgcggcg gcggccgcat attggagcgc cgcgacgcgc
    11041 ggttcgtgcc gcgcaagggc tatctccgcc gtgacggtat aaaatgtgcg cggggctgcg
    11101 gtgtcttggg atttggggcc gacgcctata caggcggtgc aggcgagcag gccaagcgcc
    11161 aacgaagccc ttgcgaggat tgaaatcgag accacagcct cactatagcg aaatgaccac
    11221 cccgatgccg ctgattcctc cttgatatcc ggcaacctct tccaccagaa tcaacaggct
    11281 acggccaaaa agcacgtcaa tggcattcaa aatcctcggc atcaatcaca agaccgcacc
    11341 ggtcgcgctg cgcgaaaagg tggcgttcag cgaggagcga ttgctgaccg cactgcaaac
    11401 tttgcgacag gaggcgggtg ttgccgaggt cgtcatccta tccacgtgca atcgcacgga
    11461 agtgtactgg gcgggatcgg cgagcggcga ggagctgtcg ctttggctcg aacgccacca
    11521 tggcaacaac ttggacctgg ccgcgagcct ctatgttcac caggagcaac gtgccgtgga
    11581 gcacgccttc agcgtcgcat cgggtctcga ctccatggtg ctcggcgagg cgcaaatcct
    11641 cggccagctg aaagatgcgt atcgcgtggc gcaggcagcc ggcagcaccg gaccctcgct
    11701 caacaagctg ttccaggcag ctttttccgc cgcgaaacgg gtgcgttcgg agacccgaat
    11761 cggcgagaac gcggtgtcga ttgcctcggc gagcgtgagc ctggcgcggc gggtgtattc
    11821 ggacctatcc gcgcgcaccg ctctgctggt cggcgcgggt gaaatgaacg cgctcgtggc
    11881 gcgccatttt gcgagcgccg gcgtgcagcg catggtgatc gccaatcgca cgcgtgcgcg
    11941 cgccgaggcg ctggcggctg aactcaaggc tcacgccgtc ggcctcgatg aactcgacac
    12001 ggaactggtg caggccgata tcgtgatcgc ttgcaccgcg agcgccgtgc cgctaatcgg
    12061 caagcgggcg gcggaaagcg ccatccgcgc acggcgccgg cggccgattt tcatggtcga
    12121 cctgggcgtg ccgcgcgata tcgacccggc tatcgccgat ctggaagatg tgtatctatt
    12181 ctccatcgat gatctgcagc agctggtcga agaaaaccgg cagcagcgcg aggtggcggc
    12241 gggtggcgcg cggctcgtca tcgacgagga agtcgcacgc ttcctcagcg aatcgcgcgc
    12301 ccaagatgcg ggtcccgcga ttcgagcgct gcgccagcag gccgaaggaa ttcgctcgca
    12361 atcgctcgaa caagcgcgac gcatgctcgc cgccggcaaa accaccgacg aagtcatcga
    12421 gtatctcgcc aataccctca ccaatcggct gctgcacaca ccgacccagg cgctgcgcca
    12481 ggccgctgaa ctcgaggatt cctccttggc gcaagcgctg acccgcctct tgatctccta
    12541 taatccgccg ccatgaaaga ctccatccgg cgcaagctcg aaaaactcga cgagcgattc
    12601 gaggaaatca gccggcttct gtcggatgcg ggcgtcattg cgcgtcaaaa ccagttccgc
    12661 gagttgtcga tggaatacgc gaaattgggg ccgttggtcg aacgctaccg gcgcttcatc
    12721 gcgctcgaag ccgaccttgc catcgcgcag gagatggcct ccgacaagga tgcggcgacc
    12781 caggcgatgg gtgaagagga gctcgcggag ttgcagccgc gccttgcggc cgaagagctc
    12841 gaactgcagc gcctgctggt gccgaaggac ccgcaggacg acagcaacat cttcttggaa
    12901 atccgcgccg gtacgggcgg cgacgaggcc gcgattttcg ccggcgattt gtttcgcatg
    12961 tatgcacgct atgccgaatc caagggctgg gaggtcgaga ccttgagcga aagtccgggc
    13021 gaacacggcg gctacaagga agtcatcagc cgcattatcg gacgcggcgc ctactcgctg
    13081 ctcaaattcg aatccggcac gcaccgtgtg cagcgcgtcc cagccaccga ggcgcaggga
    13141 cgcattcata cctcggcctg caccgtggcc atcctgccgg agttggcgga agtcgaggcg
    13201 atcgagctca atccctcgga tctgcgtatc gacacgtttc gcgcctcggg tgccggcggc
    13261 cagcacgtca acaaaaccga ttcggcgatt cgtatcaccc atatcccgac cggagtggtg
    13321 gtcgaatgcc aggacgagcg ctcgcagcac aagaatcgct cgcgcgccat gtccttgctg
    13381 aaatcccgcc tgctcgcggc cgagcgcgag aaacagcaaa gccagcaggc acagtcgcgc
    13441 aagctccagg taggctcggg cgatcgctcg gaacgcatcc gcacctataa ctttccccag
    13501 ggccgcgtca ccgatcaccg catcaacctg acgctttaca agctcgatga ggtcatgaac
    13561 ggtcatctcg aggaattgat ccgcgccctg aaccaggagc accaggccga agagctggcg
    13621 caccaagtcg agtgaacacg gtcgccgaat ccatctcccg agcggcgcga tcgctctcga
    13681 gccacagcga atcgccgcgg ctcgatgccg aattgctgct cggcaagatc ttgggcttat
    13741 cgcgcgccgg gctgatcgcg cgcagcaacg agcccgtcag cggcgaatcc cagaagagcc
    13801 tggcgaactt gctcgaggag cgccggcgcg gcacgccggt tgcctacctc accggcaccc
    13861 gcgagttctg gtcgctggcg ctgcacgtga cgcccgccgt gctcgtgccc cggccggaga
    13921 ccgaaatcct ggtcgatctc gcgctgcagc gcttgccgga ggaccaggcg gccacggttc
    13981 tcgatctggg aacgggcagc ggcgccatcg cgctcgcgat cgccgccgag cggccgctgt
    14041 cgcaggtgac gggcgtcgat atttctgcgg cggcactcga gatcgccaga cagaatgcgt
    14101 ccgctctgca tctcacgcgc atcgactggc gcctgggttc ctggtttgcg ccggtcgtta
    14161 agcagcgctt cgatatgatc gtcgccaatc caccgtacat cgccgccgcc gatcccgcgc
    14221 tcgaaagact ggcggcggag cccgcgatcg cgctctgcga cgggcccacg ggattggagg
    14281 ccctgtcgac catcgccgcg ggcgccgcgt cgcacttgac tcggcacggc tggctgttgc
    14341 tcgagcacgg cagcacgcag ggtcccgagg tcgcgcggct gctgcacagt cacggcttct
    14401 cgtccgttcg ctcccatccc gatttttcag gcaaactccg cgtcacgctg ggcacccttc
    14461 actcaccacc ttaggaaccg acatgatccg cttcgaaacc aacctgggcg acttcaccgt
    14521 tgaactgttc gagcaggacg cgccgctatc ggtggcgaac ttcttgcgct acatcgacga
    14581 tggatttttc gacggcacga ttttccatcg catcgttccc ggattcgtca tccagggcgg
    14641 cggcttcacc gaagacatga cgcagaagaa gacgcatgct ccggtcaaga acgaggccga
    14701 taacggccgc aagaacgcgc gcggctcgct gtccatggca cgcaccaacg acatcaacag
    14761 cgccacctcg cagttcttcg tcaatttgaa agacaacgac tttctcgatc actcgcgcgg
    14821 caacttcggc tacgcggtgt tcgcgcgcgt caccgacggc atggacgtca tcgacaaaat
    14881 cgccgcggtc gagaccggcc gcaagcgcgg cttcgacgac gttccggtgc aggccgtcat
    14941 catgaaatcg gtacggcgca ttcctgcttg acgccttaag tggcgcgcga tcggtcaatc
    15001 ttcaggcggc tgctgcgggc gatcgcgtgg ctgatcgggc tcggcctttt ggcaagcgtg
    15061 gctgcggtgg cggcgttgcg ctggatcgat ccaccgttca cggccttcat ggccgaggcg
    15121 caggccgagg cgtgggcgca tcgcgactcg agctatgtat cgcgtcacag ctgggtcgat
    15181 ttgcagcgca tttcgcccaa tctgccgctg gcggtggtgg cgtccgagga tcagaagttt
    15241 cccgaacact ggggcttcga tgtcgaggcc atcgagaaag cctacgcgct caatcagcac
    15301 agccacaagg tgcgcggcgc cagcaccatc agtcagcagg tcgccaagaa tgtgtttctc
    15361 tggtcgggac gcagctattt tcgcaagggc ctcgaggcct atttcacggt cttgatcgaa
    15421 agcctgtggc cgaaaaggcg catcctcgag atctacttga atatcgccga atttgggccc
    15481 ggcacctacg gcgcggaggc tgccgcgcaa cgcttcttcc acaagacggc ggcacagctc
    15541 acgcgcggcg acgcggcgct gctcgcggcc gtgctgccga accccgaacg ctattcggtg
    15601 tccgcgccat cgcgcttcgt gcagcagcgg cgcgaatgga tcctgggtca gatgcaggcg
    15661 ctcggcggcc ctgaaatgct cgacgaaatc gacgcctacc caaaccggcg ccgctgatcg
    15721 cagagctcgc ggcatactgt tgagtcaacg ccggccgcga tttgccgcgc cggccggctc
    15781 gaggtctgcg cttgctcgcg ttcgaggatt cgctgcagga tcggcggtat ctcgaaaata
    15841 gcgcgattgc ggatgcgcgc cactcggccg cgatactcgt tcatgccgga cgtgagggtc
    15901 gcgacggccg cccgaatcgt gcgaaacgaa tcgagcacga ggccggcacc gttctcctcg
    15961 acccagtgcg tgttgtagcg ctcttgcggc atggtcgcgg cgttgcgcac gatgatgacg
    16021 gggagctgtt gctgaatggc ttcgctgatg ctgccggggc cgggcttgcc gatgaagaag
    16081 tcgctcaact gcatgaacaa accgatttgc gccgtgaagc cgatgaccgc gcgcggcgcc
    16141 gccgaattca tcgcgcgcaa atcgtccgcg agctcgcagt tgtggccgca gatgagaatc
    16201 agctgggtgt cgtcgaggcg ccgcgcgatc cggcgcatcg ccttcgatcc atggccgccg
    16261 aacatcacca tgcccgtcgg acgatccggg tcgagcttga gcttggcgcg ctcggcgcgc
    16321 cgctcgatgc gcaaatttcg gtagaaatcc ggacggatga tcatgcccga ggtcgcatga
    16381 atgcgagtgt cgtcgtagcc cgcggcccgc gcctggagaa ccgccttggg cgtgccgcag
    16441 atcaaatgct gcgcctggtg cggctcgatc caaaaatgcg gcggtgagtc ggcgaagtcc
    16501 gtgaggatgg tgacgaacgg agcattcggt cgtgccgccg tcagcgcctg gtacatggcg
    16561 cgattgaagt tcggcaccag cgagaccacg aggtcgggca gggttcgctg ccagtgacgc
    16621 cgcaagcgcg cggtcagggt tctatgaccg aggcgaatga gcgcctgaaa cactttcaac
    16681 tcctgggtca gccccaaggt ccagccgcgc gccagccgcg cgttgtaata atcctcgggc
    16741 ttcattcccg tggtcttggc gaacacgtcc ttgggatcga gcacttcgaa gagattgacg
    16801 agccgcacgt gccagggcag cgccaaatcg cgaatcgcca tttcgagggc cacggctgcc
    16861 gagcggtgac cgccgcccgc gttgaaatac acgagatcga tagacttcat gcgcgtcatt
    16921 ttgcgtcagg actatgacca aaccgtgaat cgtcctgcta ccctgtccca cttttgcgaa
    16981 ggagtccgga tggttgtttc ctgcgcggcc cgagccggtt ggctgttggc cgcgagcctg
    17041 ctgctggccc cgaccgcgga ctttgcggct gagccgacac ccgctgcgcc gacccagttg
    17101 agccacggcc gcttcaagga cttctcggtt ttccagccga agggcgtgcc gaccagcttc
    17161 gtgctgttcc tgtcgggcaa cgagggttgg aatgccgccg ccgataccct cgcccgccag
    17221 ctcgttcagc acggcgccat ggtcaccgcc atcgactgga tcaagctaaa agcagcgttt
    17281 gaagcggacg gcgatcaatg cgtttttccc gatggcgatc tcgagaactt gagccatttc
    17341 gtgcaggcct attttcacac gcccacgtat ttgtcgccgc tgttggtggg agtctcttcg
    17401 ggcgcagcga tgtcctatgc catgctggcg caggcgccgc gcaacacctt tgcggccgca
    17461 ctgactctcg gattctgtcc gaacctcgat ttggagaagc cgctctgcaa ggggtccggc
    17521 gtcgaattta cgcgcagcga gcacctgcgg ggcgtcgagt tgttgccgaa caagagcctc
    17581 ggtaacccct ggctcgactt gcaaggcgca gacaatcgca actgcccggt ggcgacggta
    17641 cgaaacttta tttcccaggc ccgcggcgcg gcgctcgtga cgctgccgca ggcgagccat
    17701 gacttttcct cgctcgcgag ctggctgccg ccgtttaccg ccgcatacga ccggctcgcg
    17761 gcacacaacc cgacccgcca agcggcgccc ccgccggccg gcttgagcga tctgccgctg
    17821 gtggaagtcc cggtgcggcc tggcgccccc gccgccgata gctttgccat catcatgtcc
    17881 ggtgacggcg gttgggcggg actcgaccag gatgtggccg ccgcgctcac cgcccaaggc
    17941 attccggtgg tgggattgga ttcgttgcgc tattactgga cggcccgcac accgcaggga
    18001 ctggccgcgg acaccgaccg gatcattcgc tactacctcg cgcactttgg caagcagcgc
    18061 gtgttattga tcggctattc gcagggcgcc gatgtgctgc cgttcgcggt caaccggctg
    18121 ccgccggcga cgcgcgcccg cgttgcgctc gccgccgtca tgggaatgtc cgagcacgcg
    18181 ctattcgaat ttcatttgag cagctggatc tcggacagcg actcgggacc gaagacgctg
    18241 ccggaaatcg atcgaatcgc cggcgtaccg gttctctgca tctatggcgc ggacgaacac
    18301 gactcgctgt gcccgcaact cgatccgcaa cgattcaagg tggtgaaatt gaagggtggc
    18361 caccacttcg acggcgatta cgccaacctg gcgcttcaaa tcctcgccgc cgctcatcca
    18421 tgaaacgccc gctatgcggc gtctgcggtc gcggcgtccg tcgccgccgc ctcgcccggc
    18481 agcgtctcgc tcgcttccgg ctcgtatccc agattgggcg cgagccagcg ttcggcttct
    18541 gcgagcgtcc actttttgcg ccgcgcgtag ctcgccactt gatcgtggtc gatttggccg
    18601 acaccgaagt aatgcgcctg cgggtgcgag aaatagaacc cgctcaccgc ggcggtcgga
    18661 tacatcgcaa acgattccgt cagccggatg cccgcgttct gttccggatc gagcagctgc
    18721 cagagcgtgg ccttttccgt gtgttcgggg caggccggat agccgggcgc cgggcgaatg
    18781 ccctggtact gctcgcggat caatccctcg ccatcgagcc gctcgtcggg cgcgtagccc
    18841 cacagctcgc gccgcacccg ctcgtgcatc cgctcggcca atgcctccgc gagccggtcg
    18901 gccaacgcct tcagcatgat gctgctgtag tcatcgtgcg ccgcctcgaa gcgtgcgacg
    18961 tgtgcatcga tgccgatgcc cgccgtcacg gcaaacgcgc ccacatagtc gctgcggccg
    19021 gagccgatgg gcgcaatgaa atcggcaaga caggactgcg cctgattctg cggcttggat
    19081 ttctgctgcc gcaaatgatg cagccgaact cgcacggtat gacgcgtctc gtcgctgaac
    19141 accgcgatgt catcgtcgct gccgttggcc ggaaagaagc ccaccacggc acgcgccttg
    19201 agccatctct cttgcacgac ggcttgcagc atacgcgtgg catccgcata cagtgcgctg
    19261 gccgctgcgc cgaccacggg atcttgcaaa atgtcgggga acttgccggc gaactcccag
    19321 gcattgaaga acggcatcca atcgatatac ggcagcagct cggcgagcgg atagtcatcg
    19381 aa
//
LOCUS       DASWZT010000350         3184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_17248, whole genome shotgun sequence.
ACCESSION   DASWZT010000350 DASWZT010000000
VERSION     DASWZT010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17248"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VGE92_09785"
     CDS             <1..381
                     /locus_tag="VGE92_09785"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEY1314158.1"
                     /translation="VFAFVGQHGLAAALFTFIIAHGPVELSVICIAGATGTVLGESLI
                     RPALATRRDSFQACAQRVGPLLLLCAALLVGCGFIEGFISPDPSFPLANRIAIGALYW
                     AVMCILMSGRLFRRAAPATDATHA"
     gene            complement(398..1696)
                     /locus_tag="VGE92_09790"
     CDS             complement(398..1696)
                     /locus_tag="VGE92_09790"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY1314159.1"
                     /translation="MSLRSNSLLLIVLMALLAVLGEWSAEPVLTDLWMLPAALLLLGL
                     AYESWIVSRSGLSLEIDPPERFLLGRAGRVKFVCTLGLRRTLRIQLAPSAPDTFDIDG
                     RIFDLRIDAGASAVVERLVSPNRLGGYPWPTSRARIAGPLRLAWWPKLLAPALQVRVQ
                     PDLFHSKAEVTGIAAAGVESGSEHGTGAEVLRLRDYRAGDPPRIVDWKATARAGRMIS
                     RDFAQDHRLEIVIVVDAGRASALRAGSLDRFGHYVNVAARLGQYAAGQDDLVGLVVYA
                     DRPLAVLAPARGAPAVARLRELLANARVERTESNPLYAAMRVRSLVRQRSLIIMLTDV
                     DDTTVASQLAQAVRLLLPKHLPFVAGLSSAAAESMARAPANHWLDPYRSLAAQEYCAG
                     LERKVRALNALGAPALVARPDGLERAVFAAYTNFRRAKRV"
     gene            complement(1702..2646)
                     /locus_tag="VGE92_09795"
     CDS             complement(1702..2646)
                     /locus_tag="VGE92_09795"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY1314160.1"
                     /translation="MSTALEALEQQLQETLGRSVIGLRSVIRSLTIAIVARGHVLLQG
                     PPGLGKTLLSKSLAAALGGTFNRVQGTADLMPSDIIGVHVYDAAKREFEFRPGPLFAD
                     VLLVDEVNRASPKTQSALLEAMEERQVSVERRTYALPPQFLVIATQNPGDFEGTYPLP
                     ESQLDRFMLRIDMSYPAREDEQSVLTQYATLAVPSSGQCAAVATPAALDAARACVDQV
                     HMAPQLLTYVLDLAQASRQHAHLTLGLSTRGALAALRAARISAALRGADFISPDDVKE
                     MLPVVISHRLVLAPEAMLEGVTTQAIVQRLLEQVPVPR"
     gene            complement(2643..>3184)
                     /locus_tag="VGE92_09800"
     CDS             complement(2643..>3184)
                     /locus_tag="VGE92_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314161.1"
                     /translation="FSNRALGEQDNARLLSNLIAWSRQPDGAVIFDDAHQGVVSYYDS
                     KAFFADPRLHSSLGWIVFLWFVFVLGVQRLRSRVAVANPADITAFVSSSGEFFAATLT
                     PAAAAARLLGNFFNAIRARLGLSQDGTPEWQWLAAQAQVSARDLAQLRRLHQGTQAGR
                     RIDLRELQNLLVQLQGKVL"
BASE COUNT          581 a         1066 c         1014 g          523 t
ORIGIN      
        1 gtgttcgcgt tcgtcggcca gcacggattg gcggcagcgc tcttcacgtt catcatcgcc
       61 catgggccgg tcgaactgtc ggtgatctgc atcgcgggcg caacgggcac ggtgttgggc
      121 gagtccttga tccgcccggc gctcgccaca cgccgcgact ccttccaggc gtgcgcgcag
      181 cgtgtcgggc cgctgctctt gttgtgcgcg gcgctcttgg tcggctgcgg attcatcgaa
      241 ggcttcatct caccggatcc gagcttccct ctcgccaacc gcatcgcgat cggtgccttg
      301 tattgggcgg tgatgtgcat tctgatgagc ggccgtctat tcagacgcgc cgcgcccgcc
      361 accgacgcaa cgcacgcatg agcgcgcgcg ccccgagcta gacgcgtttc gcgcgacgga
      421 aatttgtgta ggccgcaaac accgcccgct cgagcccatc gggcctcgcg accagtgccg
      481 gcgcacccag cgcattgagg gcgcgaactt tacgctccag acccgcgcaa tactcttgtg
      541 ccgcgaggct gcggtaggga tcgagccagt ggttcgccgg cgcacgggcc atcgactcgg
      601 ccgctgcgct cgacagtccg gcgacgaacg gcaaatgctt ggggagcagc aggcgcaccg
      661 cctgcgccag ctggctggca accgtcgtgt cgtcgacgtc ggtgagcatg atgatgagac
      721 tgcgctggcg caccaagctt cgcactcgca tcgcggcgta caacggattc gattccgtgc
      781 gctcgacgcg tgcattcgcg agcaactcac gcaggcgcgc aaccgcgggt gcgccgcgag
      841 ccggcgccag caccgcgagg ggccgatcgg cataaacgac cagtccgacc aaatcgtcct
      901 gtcctgccgc atactggccc aagcgcgccg cgacattgac gtaatggccg aaacggtcga
      961 gcgagcctgc gcgcaatgcg ctcgcgcggc cggcatcgac cacgatgacg atttccaagc
     1021 ggtgatcttg cgcaaaatcg cggctgatca tgcgcccggc gcgcgccgtc gccttccaat
     1081 cgacgatgcg cggcggatcg ccggcgcgat agtcgcgcag gcgcaaaacc tcggcgccgg
     1141 ttccgtgttc gctgcctgac tcgacaccgg cggcggcaat gcccgtgact tccgccttgc
     1201 tgtgaaacag atcgggctgg actcgaacct gaagtgccgg cgcaagcagc ttgggccacc
     1261 acgcgagccg cagtggcccc gcaatacgcg ctcgcgacgt cggccacggg tagcctccca
     1321 gccgattcgg actcaccagc cgctcgacca cggccgacgc cccggcatcg atacgcaagt
     1381 cgaagatacg gccgtcgatg tcgaaagtgt cgggcgcgct cggcgcaagt tgaatgcgca
     1441 atgtgcggcg cagcccgagc gtgcacacga atttcacgcg gcccgcgcgg ccgagaagaa
     1501 accgttccgg cgggtctatt tcaagactca gtccgctgcg cgatacgatc caggattcgt
     1561 aggcgagacc gagcagcagc agcgcggcgg gcagcatcca taagtccgtg agcaccggtt
     1621 ctgcgctcca ctcaccgagc acggcgagca gcgccatcag cacgatgagt agcagtgaat
     1681 tgctgcgcag gctcatacgc tttagcgcgg caccggcact tgctcgagca gacgctgcac
     1741 gattgcctgc gtggtcactc cttcgagcat ggcctcggga gccaacacca gccggtgaga
     1801 aatcacgacc ggcagcattt ccttcacatc gtccggcgat atgaaatcgg cgccgcgcag
     1861 cgccgcactg atgcgcgcgg cacgcaacgc cgccagggcc ccgcgtgtcg acagacccag
     1921 cgtgagatgc gcgtgctgcc gcgaggcctg cgccaaatca agcacatagg tcagtagctg
     1981 cggcgccatg tgcacctggt cgacgcaggc tcgagcggca tcgagggcgg cgggcgtcgc
     2041 gaccgcagcg cattggccgc tcgagggcac ggcaagcgtc gcgtactgtg tcaagacaga
     2101 ttgctcatct tcccgcgccg gatagctcat atcgatgcgc agcatgaacc gatccaactg
     2161 cgactccggc agcgggtagg tcccttcgaa atccccagga ttctgcgtgg cgatgaccag
     2221 gaactgcggt ggcagcgcgt aggttctgcg ttcgacggat acctgccgct cttccatcgc
     2281 ctcgagcagc gcggactggg tcttggggct ggcgcggttc acttcatcca ccagcaaaac
     2341 atcggcaaac aacggtccgg gccgaaactc gaactcgcgc ttcgcggcgt cgtagacatg
     2401 cacgccgatg atgtcggacg gcatcaggtc ggcggtgccc tgcacgcgat tgaaagttcc
     2461 accgagcgca gccgccagag acttgctcaa cagcgtcttg ccgagtcccg gcggcccctg
     2521 cagcaataca tgcccgcggg ccacgatggc gatcgtcagg gagcggatca ccgaacgcaa
     2581 tccgatgacc gatctgccga gcgtctcctg caactgctgc tccaacgctt ccaatgccgt
     2641 gctcataaga cttttccttg caactgaacc agtagattct gcaattctcg caaatcgatc
     2701 ctgcgcccgg cctgcgtccc ttggtgcagt cggcgcaatt gcgcgagatc ccgcgccgac
     2761 acctgcgcct gggcggcgag ccattgccat tcgggcgtcc cgtcctggct caaacccaag
     2821 cgagcgcgga tggcattgaa gaaattgccg agcagccgtg ccgccgccgc tgccggcgtc
     2881 agtgtcgccg caaaaaactc gccgctgctg ctgacgaagg cggtgatgtc ggcgggattg
     2941 gccactgcga cgcggctgcg caagcgctgc acgccgagca cgaacacgaa ccacaggaag
     3001 acgatccagc cgaggctgct gtgcaagcgc gggtctgcga agaaggcctt ggagtcgtaa
     3061 tagctgacga ccccttggtg cgcatcgtcg aatatgacgg cgccgtcggg ttgccgcgac
     3121 caggcgatga gattcgagag caggcgggca ttgtcctgct cgccgagcgc acgattgctg
     3181 aaaa
//
LOCUS       DASWZT010000351         1974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_12548, whole genome shotgun sequence.
ACCESSION   DASWZT010000351 DASWZT010000000
VERSION     DASWZT010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12548"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..>1974
                     /locus_tag="VGE92_09805"
     CDS             38..>1974
                     /locus_tag="VGE92_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1314162.1"
                     /translation="MSGHSFVPNGSVATTPPAVYNSQQYIFMTREDDRYNAAFMAHDD
                     INEYFKPYAEFFFMDDKTHQQVAPAALFKDSNPLDPTGAGDYYINCSNPLLSPQQAGI
                     LCTPAQIAADKANPGSTTVQVRIGRRNIEGGDRYIDFDHTNYRTVLGTKGDFADAWSY
                     DVYGQYFYTAFSDSNQKYLSYQGITNALLVTGSAANPTCIGAPRGCVPYNIFTDGGVT
                     QAALNYLYQLGTAQGASQLRTLHADVTGKLGNYGIQSPLAAEGVGVNLGFEHRNDHEY
                     LQPDSAEESGLLSGFGSAVAPIDNSISVKEEFLEVRAPLVQDRAGAKELLFDTGYRRS
                     DYSTAGVTNTYKFEVQYAPITDYRIRASYDRAIRAPSVAEAFTPPIVGLAAVGNDPCA
                     PPITFNLQQCERTGVTAVQFNSGSIPQGTADQLSQETSGNSALKPEQADTYTLGVNFS
                     PSQIPHLTGSIDYYHIQVKDEIGVIPYLVILSNCANTGDPTYCNQIVRSPSTGSLTGN
                     STTSGGYVIQKDYNLGTAVVSGIDVQMSYKLDLPPSFGDVSFSLNGAYLLHNETQPLP
                     GAHTYDCAGLFGETCQTVDPRWHHLFRTTWDTPWNISTSLTWRYIGSVSQDNNSSDPT
                     LHFATWGAYDYFNAKIGAYNY"
BASE COUNT          421 a          671 c          542 g          340 t
ORIGIN      
        1 gccgaccgcc ggcgcgaacg cagggacggc ttacagcgtg tccgggcata gtttcgtgcc
       61 gaacgggtcg gtcgcgacca cgccgcccgc ggtctacaac tcccagcaat atatcttcat
      121 gacgcgcgaa gacgatcgct acaacgcggc gttcatggcg cacgatgaca tcaacgaata
      181 cttcaagccg tacgctgaat tctttttcat ggatgacaag acgcatcagc aagtcgcacc
      241 ggcggccctg ttcaaggaca gcaatccgct cgacccaacc ggtgccggcg actactacat
      301 caattgcagc aatccgttat tgagtccgca gcaggcaggg atcctgtgca cgcccgcgca
      361 aatcgcggcc gacaaagcca acccgggatc gaccacggtg caagtcagaa tcggccgccg
      421 caacatcgag ggcggcgacc gctacatcga tttcgaccac accaactatc ggaccgtatt
      481 gggcaccaag ggcgacttcg ccgatgcctg gagctacgat gtctacggac agtatttcta
      541 taccgcattt tccgattcca accagaaata cctgagttac caagggataa ccaatgccct
      601 gctggtgacc ggcagcgccg ccaacccgac ctgcatcggc gccccccgag gttgcgtgcc
      661 gtacaacatc ttcacggacg gcggcgtcac ccaggccgcg ctcaattacc tgtaccaact
      721 cggcaccgcg caaggcgcct cgcagctgcg cacgctgcat gccgacgtca ccggcaaact
      781 cgggaactac gggatccagt cgccgctcgc ggccgagggg gtgggtgtca acctaggttt
      841 cgaacaccgc aacgatcacg aatacttgca gccggactcc gccgaggaaa gcggcttgct
      901 gtccggcttc ggcagcgcgg tcgcgcccat cgacaacagc atttcggtca aggaggaatt
      961 cctcgaagtg cgcgcaccgc tcgtccagga tcgggccggc gccaaggaac tgttgttcga
     1021 taccggttat cggcgttccg actactcgac ggccggcgtc accaacacct acaagttcga
     1081 agttcaatac gcgcccatca ccgactatcg catccgcgcc tcctacgacc gcgcgatccg
     1141 cgcgccgagc gtcgccgagg cgttcacgcc gccgatcgtc gggctcgccg cggtcggcaa
     1201 cgacccgtgc gctccgccca tcaccttcaa cctgcagcaa tgtgaacgca cgggcgtgac
     1261 cgcggtgcag ttcaacagcg gctcgattcc gcagggcacc gccgaccagt tgtcgcagga
     1321 gaccagcggc aattcggcgt tgaagccgga acaggccgac acctatacct tgggtgtgaa
     1381 cttctcccca agccagattc cgcatctcac cggcagcatc gactactacc acatccaggt
     1441 gaaggatgag attggcgtca tcccgtatct cgtgatcttg tcgaattgcg ccaataccgg
     1501 cgacccgacc tactgcaacc agatcgtgcg ctcgccgagc accggcagcc tgaccggcaa
     1561 cagcacgacc tccggcggct atgtgattca gaaggattac aacctcggta ccgcggtggt
     1621 gagcggcatc gatgtgcaga tgagctacaa gctcgacctg ccgccgagct tcggcgatgt
     1681 ctcattctcc ctcaatggcg cctatctgct gcacaatgaa acgcagccgc tgccgggcgc
     1741 gcatacctat gactgcgccg gactgttcgg cgagacctgc cagacggtcg acccgcgctg
     1801 gcaccacctg ttccggacga cctgggacac gccgtggaac atttccacat ctctcacctg
     1861 gcgctacatc gggtcggttt cgcaggacaa caacagcagc gatccaaccc tgcatttcgc
     1921 cacctggggc gcgtacgatt atttcaacgc caaaatcggc gcgtacaact acat
//
LOCUS       DASWZT010000352         1791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_12551, whole genome shotgun sequence.
ACCESSION   DASWZT010000352 DASWZT010000000
VERSION     DASWZT010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12551"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VGE92_09810"
     CDS             <1..900
                     /locus_tag="VGE92_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY1314163.1"
                     /translation="GGLAATAQADVTDTDALAEAAHAIAANLGPIDVWINCAGNGVYG
                     RFGDVPEVEFQRVTDVTYHGTVNGTRVALAHMRSRGHGRIINVCSAIAFHGLPLMSSY
                     AGAKAAVRGFSQAVQGELKLERSPIRITTVFPPAANTPYFSHAVSHMGWPARPARPVY
                     QPEVVAQGILQAVAGRRREMTISGTVVAFSLATRIAPGLVAWGMGKMGIARQATTDPD
                     ARRLLEPTLFAPSQHVFEVHGPFGRGARQWSVHLWFERWLTLLRRVFTVTPRPPVVAL
                     PPTRDPPEPGPELAGLAAPRVES"
     gene            complement(800..1624)
                     /locus_tag="VGE92_09815"
     CDS             complement(800..1624)
                     /locus_tag="VGE92_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY1314164.1"
                     /translation="MAIVTGATGGIGRWIALGLARAGYHTVLIGRDDARTKAARAWIA
                     QRVPQASTEPMLADLSRLAATRALGEDIAARHRKIAVLVNNAGIFDATRVVTAEGQER
                     VLATNLLSPYVLTRALLPALLAGAPSRIVTVGSSTSDSARIDPDHLVLGARWTMVQAY
                     GQSKLAAMMMTFAFARQLAGTGVTANVVHPGLVASGLVRTPGIIGFAWRCMAPFALSE
                     EQGADTPLAAALAPEFANISGVYLKTRRVAPPNRQALDPVLVDRVWAATQRLADVA"
BASE COUNT          295 a          588 c          591 g          317 t
ORIGIN      
        1 ggcgggctcg ccgccaccgc gcaagccgat gttacggaca cggacgctct ggccgaagcc
       61 gcgcatgcga tcgcagcaaa cctcggtccc atcgatgtgt ggatcaattg cgccggcaac
      121 ggcgtctatg gccgcttcgg cgatgtgccg gaagtcgagt tccagcgtgt cacggacgtc
      181 acctatcacg gcaccgtcaa cggcacgcgc gtggcgctcg cgcatatgcg atcgcgcggg
      241 catggccgca tcatcaatgt gtgctcggcg atcgcgtttc acggtctgcc cttgatgtcg
      301 tcctatgcgg gcgccaaggc ggccgtgcgc gggttcagcc aggcggtgca gggcgaattg
      361 aaactcgagc gcagcccgat ccgcatcacc accgtctttc cgccggccgc gaacacgccc
      421 tatttcagtc acgccgtgag ccatatgggc tggcccgcgc gcccggctcg ccccgtctat
      481 cagccggaag tggtggcgca gggaatcctg caggccgtcg cgggccgccg ccgcgagatg
      541 acgatcagtg gaacggtcgt ggccttctcg cttgcgacgc gtattgcgcc gggcctcgtc
      601 gcgtggggca tgggcaagat gggaatcgcg cggcaggcaa cgaccgatcc cgatgcgcgg
      661 cgcctgctgg agccgacatt gtttgcgccg tcgcagcatg tgttcgaggt gcacggcccg
      721 ttcggacgcg gcgcgcggca gtggagcgtg catctgtggt tcgaacgatg gctgacgttg
      781 ctcaggcgcg tcttcacggt tacgccacgt ccgccagtcg ttgcgttgcc gcccacacgc
      841 gatccaccag aaccgggtcc agagcttgcc ggtttggcgg cgccacgcgt cgagtcttga
      901 gatagacgcc gctgatgttt gcaaattcgg gcgcgagggc cgcggcaagc ggtgtgtcgg
      961 cgccttgctc ttcgcttagc gcaaaaggcg ccatgcaccg ccacgcgaaa ccgatgatcc
     1021 ccggcgtgcg caccagcccg ctcgcgacca atcccggatg caccacattc gccgtcacac
     1081 cggtgcccgc gagttgcctg gcaaacgcga acgtcatcat catggccgca agcttggatt
     1141 ggccataggc ctgcaccatc gtccagcgcg cgcccagcac gagatgatcg ggatcgatcc
     1201 gcgcgctgtc ggacgtcgac gaaccgaccg tgacgatgcg cgacggcgca cccgcaagca
     1261 gcgccggcag caaggcgcgg gtgagaacgt acggcgagag caaattggtg gcgagcacgc
     1321 gctcttggcc ttccgcagtg acgacccgcg tcgcatcgaa gatgcccgca ttgttgacca
     1381 gcacggcgat tttcctatga cgcgccgcga tgtcctcgcc caaggcgcgc gtggcggcga
     1441 gacgcgagag atccgcgagc atcggttccg tgctcgcctg cggcacgcgt tgtgcgatcc
     1501 aggcccgcgc cgccttagtg cgcgcatcgt cgcgtccgat caggacggta tgatagcccg
     1561 cgcgcgcgag ccccagcgca atccagcggc cgatgccgcc ggtcgcgccg gtcacgattg
     1621 ccacgggcag ttctgcgctg atggtggatt actctatctg aggctgcgca ttcatcttaa
     1681 cacttgatac agactgaagg acgttatggg aattgttcca ttgacgaatg ggcttcaact
     1741 gttcgggctt ggcgcggttt cgctgatgct cgtctgctac gcgctggaat c
//
LOCUS       DASWZT010000353         1881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_75244, whole genome shotgun sequence.
ACCESSION   DASWZT010000353 DASWZT010000000
VERSION     DASWZT010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75244"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..301
                     /locus_tag="VGE92_09820"
     CDS             23..301
                     /locus_tag="VGE92_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEY1314165.1"
                     /translation="MQQSTRRFLVFGKVQGVFFRQSTRLEAERLGIRGIARNLSDGSV
                     EVLAHGAADALEELRVWLQRGPAHARVEEVRETPAHGADPVPGGFTTI"
     gene            complement(312..899)
                     /locus_tag="VGE92_09825"
     CDS             complement(312..899)
                     /locus_tag="VGE92_09825"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY1314166.1"
                     /translation="MRRPLRAKLACALLLTGCCAVLGRAHADQQRDPELKALLQKIIG
                     SADCFADKYESEVWYKAMEPRLARLVPTHEARVEILDHVYCEAKRDPTLQLPPDLVLA
                     LIEVESRFDPWAVSPAGAVGLMQVMPFWPRELGVQNQLVRVAPNIRMGCEILRYYLRV
                     ENHDWTRALARYNGSVGRNTYPALVMQRWQRAWRF"
     gene            complement(910..>1881)
                     /gene="proS"
                     /locus_tag="VGE92_09830"
     CDS             complement(910..>1881)
                     /gene="proS"
                     /locus_tag="VGE92_09830"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEY1314167.1"
                     /translation="PAAEPLTKVATPNARTIAELSAQLGVAAARCVKTLLVDGSDGGV
                     VALVIRGDHELNAVKSQKLPGVANPLRMASAERVRQATGAEPGFIGPIGFGGTIYVDY
                     AAAHLADFVCGANEKDAHFRGVNWGRDLQEPQSADIRNAQRGDPSPSGRGTLEIARGI
                     EVGHIFQLGQVYTEAMHASVLDEQGKALTMFMGCYGIGVTRVVAAAIEQNHDANGIIW
                     PEAIAPFQVVLVPINYQKSAQVRETADALYEQFSAAGIDVLLDDRDARPGVKFADSEL
                     LGIPHRIVIGERGLASGKLEYRHRSATESTEIPAADPVGYIRNKLNR"
BASE COUNT          331 a          606 c          597 g          347 t
ORIGIN      
        1 ccacccaacc gcactgaccc gcatgcaaca gtcgacgcgc cgttttctcg tctttgggaa
       61 agttcaaggc gtgttttttc ggcaaagcac gcggcttgaa gccgagaggc tcggtatccg
      121 tggcatcgcg cgcaatctat cggacggatc cgtcgaggtt ctcgcgcacg gcgctgcgga
      181 cgctctcgag gaattgcgcg tgtggttgca gcgtggcccg gcgcacgcgc gggtcgaaga
      241 agtgcgagaa accccggcgc atggggcgga ccctgtgccg ggcggattca ccacaatatg
      301 aacgcaaagg atcaaaaacg ccaggcgcgc tgccagcgtt gcatgaccag cgccgggtag
      361 gtgttgcggc cgacgctgcc gttgtacctc gccagcgccc gggtccaatc gtgattctcg
      421 acgcgcaggt agtagcgcaa gatctcgcag cccatgcgga tattgggggc gactcgcacc
      481 agttggtttt gcacgccgag ttcccgaggc caaaacggca tcacctgcat caggccgacc
      541 gcgccggcgg gcgacaccgc ccagggatcg aagcggcttt cgacctcgat caaggccagc
      601 accaagtcgg gaggcagttg cagcgtggga tcgcgcttcg cttcgcagta cacatgatcg
      661 aggatttcca cgcgcgcttc atgggtgggc acgagacgcg cgagtctcgg ctccatggct
      721 ttgtaccaaa cttccgactc gtatttgtcg gcgaagcaat cggcactgcc gatgatcttc
      781 tgcagcagcg ctttcaactc cggatcccgc tgctgatccg cgtgcgcacg gccgagcacg
      841 gcacagcatc ctgtcaggag aagggcgcag gcgagtttcg cgcgcagcgg ccgccgcatc
      901 ggtcattcct cagcgattga gcttgttgcg aatatagcct accggatccg cagcgggaat
      961 ttccgtcgat tcggtcgcgc tgcggtgccg atattccagc tttcccgaag cgaggccgcg
     1021 ctcgccaatg acaattcgat gcgggatgcc gagcagctcg gaatcggcga acttgacgcc
     1081 gggacgcgcg tcccgatcgt cgaggagcac atcgatcccg gccgcactaa attgctcgta
     1141 cagggcatcg gccgtctcac ggacttgcgc cgacttttga taattgatgg ggactagtac
     1201 cacttggaac ggcgcgatcg cctccggcca gatgatgccg ttggcgtcgt ggttctgttc
     1261 gatggcagcg gcaaccaccc gtgtcacgcc gatgccgtag cagcccatga acatggtcaa
     1321 cgctttcccc tgctcatcga gcacgctggc atgcatggcc tcggtgtaga cctgaccgag
     1381 ctggaaaatg tggccgacct cgatgccgcg ggcgatctcc aacgtaccgc ggccgctcgg
     1441 gctcgggtcg ccgcgttgcg cattgcggat atcggcggat tgcggctcct gcaagtcgcg
     1501 cccccaattg acgccgcgga agtgcgcgtc cttttcgttg gcgccgcaga cgaaatccgc
     1561 caaatgagcc gccgcatagt cgacgtagat cgtgccgccg aaaccgatcg gtccgatgaa
     1621 tccgggctcg gcgccggtcg cctggcgcac ccgctcggcg cttgccatgc gtaacggatt
     1681 ggccactccg ggcagctttt gcgacttcac cgcatttagt tcgtgatcac cgcggatgac
     1741 gagcgccacc acgccgccgt cgctgccatc gaccagcagc gtcttcacgc agcgcgccgc
     1801 agcgacgccg agttgcgcgc tcaactcggc gatggtgcgc gcgttgggcg tcgctacctt
     1861 ggtcagcggc tccgcggcgg g
//
LOCUS       DASWZT010000354        14269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_80212, whole genome shotgun sequence.
ACCESSION   DASWZT010000354 DASWZT010000000
VERSION     DASWZT010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14269
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80212"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..2866
                     /gene="polA"
                     /locus_tag="VGE92_09835"
     CDS             95..2866
                     /gene="polA"
                     /locus_tag="VGE92_09835"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005095822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEY1314168.1"
                     /translation="MSARKLILVDGSGYLYRAFHALPPLSNSRGEPTGAVLGVLNMLN
                     KMVKEESPDRIAVVFDAPGRTFRDDLFEKYKAHRPAMPDDLRSQVQPLYDTVEAMGLP
                     LLRVPGVEADDVIGTLAKQGAASGYEVLISTGDKDMAQLVGPHIGLINTMSNSRLDRA
                     GVKAKFDVFPEQIIDYLALVGDSSDNIPGITGVGPKTAAKWLNQYQTLDELIAHAGDI
                     GGKVGDNLRGELAMLELSRKLATIDTSVPLHLDADGLTAGAPDLARLRELYTRLELRA
                     LLKALGPQLAAPVNEPSAAKIDVVVAASVVADGAAAVALVDASAAAHTYHKILSQDVL
                     QAWLDKLAEAPLISFNVETDSRDYMQAHIVGLSFALAPGEAAYVPLGHDYPGAPQQLD
                     RDKVLEAFKPLLEDSTRPKLGHHQKYDAHALANHGIRLDGQRFDSMLESYVLNSVATR
                     HDMDTAVAKYLGIKTIHYEDIAGKGAKQISFNQVDVDRAADYSATDADVTLQLHHALW
                     PQIAALPTLKTVYESIEQPLVAVLYRMERSGVLVDRALLKTQSSELAARMLELQTQAH
                     AEAGGVFNVDSPKQLQEILFGKLGIPVMRKTPTGQPSTAEDVLEELAESYPLPKLILE
                     YRSVTKLKSTYTDTLPEQINPATGRIHTSYHQAVAATGRLSSTDPNLQNIPIRTKEGR
                     RIRQAFVAPPGHSLVAADYSQIELRIMAHLSGDANLLQAFAEDRDIHQATAAEVFAIP
                     LEEVHADQRRSAKAINFGLMYGMSAFGLARQLGIARGEAQKYMDVYFERYPGVKRYME
                     ETRRQARETGYVETVFGRRLYLPEIQSRNAALRQYAERSAINAPMQGTAADIIKRAMI
                     EVDAWLQRSQIPARLIMQVHDELVLEVADAAIEAMTGPLRSYMAQAATLAVPLKVDVG
                     VGRNWDEAH"
     gene            complement(3242..3832)
                     /gene="yihA"
                     /locus_tag="VGE92_09840"
     CDS             complement(3242..3832)
                     /gene="yihA"
                     /locus_tag="VGE92_09840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEY1314169.1"
                     /translation="MSIYPHVKFLTSAAESHQLAPDVGREIAFAGRSNSGKSSAINAI
                     TQRSGLARVSRTPGRTQLINFFELAPERRLVDLPGYGFAKVPERVRLHWLQLMEHYFN
                     VRESLVGLVLIVDSRRGLGEQDVGMLEWVLARDRLAHVLLTKADKLNQRDGQRVLRET
                     LAACSDTSVSAQLFSAPRKQGFEEARQVMARWFDPP"
     gene            3918..4526
                     /locus_tag="VGE92_09845"
     CDS             3918..4526
                     /locus_tag="VGE92_09845"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY1314170.1"
                     /translation="MKRLWIIATIICGAIVNNAAKPAGNVQTGATKAIVCQACHGANG
                     NSTNPEWPSLAGVGADYIAEQLKNFKEGKRANPVMMPNAMSLSADDMADLGAYFDSLV
                     NTGLEADPSYWQSGEKLYRGGDAARDIPACMACHGPTGRGNEPAKFPALRGQQSVYVS
                     KQLNDYASGARSTGPNGIMQTIAKRLSPEDIRNLASYLQGLR"
     gene            4529..5452
                     /locus_tag="VGE92_09850"
     CDS             4529..5452
                     /locus_tag="VGE92_09850"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEY1314171.1"
                     /translation="MQVRQRLLCFALALLLAACGKQESTGEHSAAVTPDSVTPAAAPA
                     PGVAAQQKKAEQAGDLPSKITRINEDGSETVEDVPSDGGTHNPLLAAVASTIAGSTSV
                     AAAATTSGPTLWQEGVNYTRLVPAQPTQVAAGQVEVLEFFWYACPHCYALDPMIESWK
                     KTKPGYISFSRVHVMWNEGHRSLARLFYTLESMGKLDQMHAEVFKEIHVNGHPLVAPD
                     ENNTAETERIQTAFVKKFGISADEFAKAYHSFAVETALQKADELEQRYRVLAVPTFVV
                     NGKYVADVHTADGPERLITLLGDLAALEHKR"
     gene            5461..5868
                     /locus_tag="VGE92_09855"
     CDS             5461..5868
                     /locus_tag="VGE92_09855"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY1314172.1"
                     /translation="MITHSGGCHCGRVRFEVIAPAKLEVADCNCSICNKSGYLHLIVP
                     ADRFKLISGRDALTTYSFNTHVAKHFFCSTCGVKSFYIPRSHPDGVSVNARCIDSGTV
                     ESMSVQAFNGRNWEQGRADYREPRSLECSANKT"
     gene            6013..6651
                     /locus_tag="VGE92_09860"
     CDS             6013..6651
                     /locus_tag="VGE92_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314173.1"
                     /translation="MRESIASLDAPPQDRAAHDVGIRDRMLLGLVLFITSVSSEALAG
                     DALSISNSVPAAADLRAATPAAGPRLEFFTVPAVGEAQTFSSTDFRPRRPTLFDSDPA
                     ANAYGNTPMLRGTTVWQRLSDYKSHDGVRLLTLWETPASSISLQAGKRGDPSLQWTSR
                     LMNHGGSTQGLLDRWFSVSLTGAGNRLRSLTRTTGTPAPAKQTDNPVVAGLK"
     gene            6808..7254
                     /locus_tag="VGE92_09865"
     CDS             6808..7254
                     /locus_tag="VGE92_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314174.1"
                     /translation="MTLTKHMVALAAFAGLAATSLTAVADEHPFTEGQVVQVSAIRTE
                     YGKFDDYMKFLDTTWKATEEAAKKAGYITGYKVITVEARGENDPDIYLVVYFKNWAAL
                     DGATAKADAISTQVEGSVAAANKGAVDRGKIRRILGSWTGQQLDLK"
     gene            complement(7338..8339)
                     /locus_tag="VGE92_09870"
     CDS             complement(7338..8339)
                     /locus_tag="VGE92_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HEY1314175.1"
                     /translation="MNWVRVVSSVALLSAIYSVGPAWADAPSANQPLPIALVDALNKL
                     SNGPHPGYRANHAKGLMVEGRFTPSPSAAAFSKATHFEKGVPVLVRFSDTTGVPTIPD
                     ADPAASPHGIAIRFTLPNGAFTDIVSISANGFPVATPEDFLALLRAAGSSGPDVPKPT
                     PIENFMNTHPAAAKFVSTRRAAPASFATLAFYGVNSFKFTNAKGVSHYARYQIIPVAN
                     EPALSDEAAKKADPNYLMDELPTRLAKGPVKFRLLAQVAGKDDSINDPTITWPADREL
                     VSLGILTLTKTVADQVQAQKLIMFSPLNLLAGIEPSQDPVLLARPAAYGVSFSQRIR"
     gene            complement(8370..9437)
                     /locus_tag="VGE92_09875"
     CDS             complement(8370..9437)
                     /locus_tag="VGE92_09875"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY1314176.1"
                     /translation="MTLGRSGLRVSPFCLGTMTFGEDWGWGSNVEDSGRIFDRYVDLG
                     GNFIDTANVYTNGHSEKILGDHLRGARAKRQRVVIATKFFGTMLNGDPNSGGAGRKAL
                     IAACDESLRRLQTDYIDLYWMHCWDVHTPIEETMRALDDLVAAGKVRYLGFSDTPAWK
                     VMEAQMLARLRGWSPLIALQLEYSLLERTIEGELVPMALEHGLGITPWSPLKSGVLSG
                     KYTRENAGQVKAGRGEWAMSALNEKSYALIDALAGIAREAGSTPARVALSWVQNRPGV
                     TSTIVGARTLQQLEDNLAALELKLAPAQSKSLDELSTPALNFPHDFVQRAAVFSSSGT
                     TINGTEAPINPLAPASDAQRY"
     gene            complement(9715..9788)
                     /locus_tag="VGE92_09880"
     tRNA            complement(9715..9788)
                     /locus_tag="VGE92_09880"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9752..9754),aa:Arg,seq:ccg)
     gene            complement(10231..10872)
                     /locus_tag="VGE92_09885"
     CDS             complement(10231..10872)
                     /locus_tag="VGE92_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016657123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEY1314177.1"
                     /translation="MISAGDALALLQDGNRRFASDDRNRDAITGRVRRLELAASQEPF
                     AVILGCSDSRVPVEIVFDQGLGDLFVIRVAGNIVAPSQIGSVEFAAEQFGTRLVVVLG
                     HTGCGAIETTLRQLQRPVENQSHNLHSIVDLIRPSVVGLLATELRHDWPALGRAAVRA
                     NIRASVKHLRHGSDIIEHLIQKAGLLVVGAEYSLETGVVDFFDGLNGPAETGL"
     gene            complement(10913..11137)
                     /locus_tag="VGE92_09890"
     CDS             complement(10913..11137)
                     /locus_tag="VGE92_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314178.1"
                     /translation="MKSLLAIIGFCLLLVLCWPLALLAIILWPLLWLLSLPFRLIGIT
                     FDAMFALLRAILFLPARLFGYRDRSSGHPS"
     gene            complement(11227..11631)
                     /locus_tag="VGE92_09895"
     CDS             complement(11227..11631)
                     /locus_tag="VGE92_09895"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEY1314179.1"
                     /translation="MSDPKEDPRLTKLAKICLELPEVGRRTLGDHADFRVRGKVFAYF
                     LNNHHGDGIVSVCCKAALGENIDRASREPRRFYLPAYIGPRGWFGLRLDRKAVDWKEV
                     RNLVELSYCLAAPKTLSRIVESATAPAAKRKR"
     gene            complement(11633..12037)
                     /locus_tag="VGE92_09900"
     CDS             complement(11633..12037)
                     /locus_tag="VGE92_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314180.1"
                     /translation="MTPIFQKLNLKAQREIAVFNVPSAFETELSKLKDVEILRDPNKP
                     RAVEFALAFAMRQAELDRLSKILAAGAVGDALLWFAYPKGTSKRYACEFNRDTGWHVL
                     RNAGFETVRQVAIDEDWSALRFRRIEYVKQGR"
     gene            complement(12065..12703)
                     /locus_tag="VGE92_09905"
     CDS             complement(12065..12703)
                     /locus_tag="VGE92_09905"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEY1314181.1"
                     /translation="MLGTHDLWLFVLSGLLLNITPGPDTLYIVGRSSTHGWQAGAVAA
                     LGVGTGVLVHVCAAALGLSAILAASATAFAAVKLIGAAYLVYVGISLLRSNSTTQSKA
                     SAADRRPAAMRGIFFQGFLTNVLNPKVALFFLAFLPQFVLSDAPSKPVPFLFLGAVFD
                     LNGTIWNLLVAWSTARISSKLATGDRFRSWFNRCVGSIFIVVGLRLALARDH"
     gene            complement(12728..13231)
                     /locus_tag="VGE92_09910"
     CDS             complement(12728..13231)
                     /locus_tag="VGE92_09910"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY1314182.1"
                     /translation="MSTPGATSSHDGGCTCRYVRYRMIGKPLFVHCCHCSWCQRETGA
                     AFALNALIEADRIQLLEGEVAAIDTPSKSGQGQRISRCPKCQIAVWSNYAGAGDALRF
                     VRVGSLDHPAGFPPDIHIFTSSKQPWVTLAPNIPAEAEYYRSAERWPAASLERRAALM
                     AKFPRKG"
     gene            complement(13228..13611)
                     /locus_tag="VGE92_09915"
     CDS             complement(13228..13611)
                     /locus_tag="VGE92_09915"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY1314183.1"
                     /translation="MSRQLISSGSQFEQDIGYSRAVVDGKWIFVSGTTGFDYATMKIS
                     DKLSDQTEQCLKNIQAALRQAGAELADVVRVTYILPDAARFPECWPILRNYFGTIRPA
                     ATMMSAGLSDPRMQIEIEATARRRR"
     gene            complement(13608..14006)
                     /locus_tag="VGE92_09920"
     CDS             complement(13608..14006)
                     /locus_tag="VGE92_09920"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY1314184.1"
                     /translation="MQISQGGCLCSAIRFRVSGEPEFSVICHCVTCRRASAATSVAWL
                     TFERARLEILSGHPHIYRSSQGVVRQFCGICGSQLFYETSGRATSIDVTTASMDNPSL
                     FPPTAEVWLEHRLAWQSAGQTRQHYPRSIE"
BASE COUNT         2954 a         4327 c         4372 g         2616 t
ORIGIN      
        1 cccgcgccca cgccgtccgg cactccggcg acagatcctc cgagcggtca agccgatagt
       61 ccttcaaagc cctgatcggg gcaggcccga gagcatgagc gcacgcaaac tgattttggt
      121 ggacggctcg ggctatttat atcgcgcgtt ccacgcgctg ccgcccctca gcaattcgcg
      181 aggagagccg accggggccg tcctgggcgt gctcaacatg ctgaacaaga tggtcaagga
      241 agaatcaccg gatcgcatcg ctgtggtatt cgatgcaccc ggccgtacct tcagagacga
      301 tttgttcgag aaatacaagg cgcatcgtcc ggccatgccg gatgacctgc gctcgcaagt
      361 gcaaccacta tacgacacgg tcgaggccat ggggttgccg ctgctgcgcg tacctggagt
      421 tgaagcggac gatgtgatcg gcacactggc gaaacaagga gcggcatcgg gctacgaggt
      481 gctgatctcg acgggcgaca aggacatggc gcaactggtc ggtccgcaca tcggattgat
      541 caacacgatg agtaactcgc ggctcgatcg cgccggcgtc aaggcaaaat tcgatgtgtt
      601 cccggagcag atcatcgatt acctggcgct ggtcggcgac agctccgaca acattcccgg
      661 aatcaccggc gtcggcccga agactgcggc caaatggctc aatcagtatc agaccctcga
      721 tgagctgatc gcgcatgcgg gcgacatcgg cggcaaagtc ggcgacaact tgcgcggcga
      781 gcttgccatg ctcgaactgt cgcgcaaact cgcgaccatc gacacgtccg ttcccctgca
      841 tctcgacgcc gacggactca ccgccggcgc ccccgacctg gcacgccttc gtgaactcta
      901 tacacggctc gaactgcgcg cgctgctcaa ggctctcggc ccgcaactcg cagcaccggt
      961 gaacgagccg tccgcggcga agatcgacgt ggtcgtcgcc gcatcggtgg tcgcggacgg
     1021 ggcggccgcc gtggccttgg tggatgcgag cgccgccgcg cacacctatc acaagatttt
     1081 gtcgcaagac gtgttgcagg cgtggctcga taagctcgcc gaagcgccgc tcatttcgtt
     1141 caacgtcgag accgatagcc gcgattacat gcaggcgcac atcgtcggcc tgtcgttcgc
     1201 cctcgccccg ggggaagctg cctacgtacc gctcggtcat gattacccgg gcgcgccgca
     1261 gcaactcgat cgcgacaaag tcctcgaggc attcaagccg ctgctcgagg attcgactcg
     1321 gccgaagctc ggccatcatc aaaaatacga cgctcacgct ctggccaatc atggaatccg
     1381 cctcgacgga cagcggtttg actcgatgct cgagtcgtac gtgctcaaca gcgtcgcaac
     1441 gcgccacgac atggacacgg cggtggcgaa gtatctgggt atcaagacca ttcattacga
     1501 agatatcgcg ggcaagggtg cgaaacagat cagcttcaat caagtggatg tggatcgggc
     1561 cgccgactat tcggccacag atgcggatgt cacgttgcag ctgcaccatg cgctgtggcc
     1621 gcaaatcgcg gcgctgccga cgctgaaaac cgtgtacgaa agcatcgagc agccgctggt
     1681 ggcggttttg tatcgcatgg agcgcagcgg cgtgttggtg gatcgcgcgc tgctcaagac
     1741 gcagagttcg gaactggcgg cgcgcatgct cgagctgcaa acgcaggcgc acgccgaagc
     1801 cggcggcgtc ttcaacgtgg actcgcccaa gcaattgcag gaaatattat tcggcaagct
     1861 gggcattccg gtgatgcgca aaaccccgac cggtcagccg tcgacggcgg aagatgtgct
     1921 cgaggaactg gccgaatcct atccgttgcc caaactgatc ctggaatatc gcagcgtcac
     1981 caaactcaag tcgacctata cggacacgct ccccgagcaa atcaatccag cgaccgggcg
     2041 catccacacg tcgtatcacc aagcggtcgc cgccaccggc agactctctt cgacggatcc
     2101 gaatctgcaa aatatcccga tccgcaccaa ggaggggcgt cgcattcgtc aggcgttcgt
     2161 ggcaccgccc ggtcactcgc tggttgccgc cgattattcg caaatcgaat tgcgcatcat
     2221 ggcgcatttg tccggcgatg cgaatttgct gcaggcgttt gccgaggatc gcgacattca
     2281 tcaggcaacc gccgccgaag tgtttgccat acctttggag gaggtgcatg ccgatcagcg
     2341 gcgttccgcc aaggccatta atttcggctt gatgtacgga atgtcggcat tcgggctcgc
     2401 gcgacagttg gggatcgcgc gcggcgaggc gcagaaatac atggatgtgt atttcgagcg
     2461 ctatccgggc gtaaaacgct acatggagga aacgcgccga caggcacgcg agacgggcta
     2521 cgtcgagacg gtattcgggc ggcgcctgta cctaccggaa atacagtcgc gaaacgcggc
     2581 gctgcgccag tacgccgaac gcagcgccat caacgcgccc atgcagggga cggctgccga
     2641 tatcatcaag cgcgccatga tcgaagtcga cgcctggttg caacgctcgc agatcccggc
     2701 tcgattgatc atgcaggttc acgacgaact ggtcctcgaa gtggcggatg cggccatcga
     2761 ggcgatgacc gggccgctgc gcagctacat ggcgcaagcg gcgacattgg cggtgccgct
     2821 caaggtcgat gtgggcgtcg gccgcaattg ggacgaagcg cactagaaaa ggcgtccgac
     2881 ccggccggtc atccgctcgg attccgggct atgcccaata gaggggggtg ggcgaagcct
     2941 gaacggcgtc catcccccgc cccggagcca aactgcatga aaagatgcac aagaaatgca
     3001 agtttggtgc aaactaacgt caataaatcg gcaggctggc cggcaatcgg ccgctgcccg
     3061 gcaaattaca aaaaatgctg atatgagggc tggaactttc ggcagagctc cccatctaac
     3121 ctaatgagcg tgaaagacgc tccccgctct tctccctgag cgggaatgac ccggtttgtc
     3181 atccccatgc cgggttggtc tgcctcggtg gctgctcaac agtaaccacc ggggcctttt
     3241 tttatggggg gtcgaaccag cgggccatga cctgccgggc ttcctcaaat ccctgtttcc
     3301 gcggtgcaga gaacaattgc gcgctcaccg aggtatccga gcacgcggcc agggtctcgc
     3361 gcagcacgcg ctgcccatcg cgctggttga gcttgtcggc cttggtcagc agaacgtgcg
     3421 ccaggcggtc gcgggcgagc acccattcga gcattcccac atcctgctca cccaagcccc
     3481 ggcggctgtc aacgatcaac accagtccaa ccagggactc ccggacattg aaatagtgct
     3541 ccattaattg cagccagtgc aggcgcaccc tttccgggac cttggcgaac ccgtaccccg
     3601 gcaggtcgac caggcggcgt tccggcgcca gctcgaagaa gttgatcaat tgcgtacggc
     3661 cgggagtgcg gctgacgcgg gccaaccccg agcgttgagt aatggcattg atggcgctcg
     3721 attttccgga attcgagcgg ccggcgaaag caatctctct gccgacgtcg ggcgcaagct
     3781 ggtgggattc ggcagcgctg gtgagaaact tcacatgggg gtagatcgac atcgccgccg
     3841 cttggacgca aattattgtc cctagtgtac aatttagggt tggcagcggt tcacattaca
     3901 tggggttacg cgcgatcatg aagcggcttt ggataatcgc aacaataatt tgcggcgcga
     3961 tcgtcaataa tgcggctaaa cctgcgggaa acgtccaaac aggagccacc aaggctatcg
     4021 tttgccaggc gtgccacggc gcaaacggga atagcacgaa tcccgagtgg ccgagcctgg
     4081 cgggggtcgg cgcggactac atcgcggagc aattgaagaa tttcaaggaa ggcaagcgcg
     4141 ccaatccggt catgatgccg aacgcgatgt cgctgagcgc cgacgacatg gcggatctcg
     4201 gcgcttactt cgacagcttg gtcaataccg gcctcgaggc tgacccgtca tattggcaat
     4261 ccggcgaaaa gttatatcgc ggcggtgacg cggcacgcga catcccggcc tgcatggcct
     4321 gccatggccc aaccggtcgc ggcaacgagc ccgcgaaatt ccccgctctt cgcggtcagc
     4381 aatccgtgta tgtatcgaaa cagctgaacg actatgcgtc gggcgcccgc agcacgggcc
     4441 cgaacggcat catgcagacc atcgcgaagc gcctttcccc cgaggacatc cgcaatttgg
     4501 cgtcctatct gcaaggcctc cgctagtaat gcaagttcgg cagcggctgc tgtgtttcgc
     4561 cttggcgcta ctgctcgcgg cgtgcggcaa acaggaatcc acgggggaac actctgcagc
     4621 ggtgaccccg gattcggtca caccggccgc ggcgcccgca cccggcgtcg ccgcgcagca
     4681 aaagaaggct gagcaggccg gggatctgcc ctcgaaaatc acccgcatca acgaggacgg
     4741 gtccgagact gtcgaagatg tgccaagcga cggcggcacg cacaatccct tgttggcagc
     4801 ggtcgcctcg accattgcgg ggtcgacgtc ggtggccgcc gccgcgacga ccagcggccc
     4861 cacgctgtgg caggaaggcg tcaactacac acggctggtg ccggcgcagc cgacccaggt
     4921 ggccgctggc caagtcgagg tgctggagtt tttctggtac gcctgcccgc attgctatgc
     4981 cctcgacccc atgatcgagt cgtggaaaaa gaccaaaccg ggctacatca gcttttcgcg
     5041 cgttcacgtg atgtggaacg agggccatcg ctccctggcg agattgtttt acaccctcga
     5101 gagcatgggc aagttggatc agatgcacgc ggaagtgttc aaagaaatac acgtcaacgg
     5161 ccatcctttg gtcgcccccg atgagaacaa cacggcggaa accgaacgca ttcaaacggc
     5221 tttcgtcaaa aagttcggta tttcggcgga tgagttcgcc aaggcgtatc actcctttgc
     5281 ggtcgagacc gcgctgcaga aagcggacga actggagcaa cgctatcgag ttctcgcagt
     5341 tccgaccttt gtcgtgaacg gcaagtacgt cgccgacgtg cacaccgccg acggcccgga
     5401 acgcctgatc acgctgctcg gcgatctggc ggcgctagaa cataaacgtt agccttcggc
     5461 atgattaccc acagcggcgg ctgtcattgc gggcgcgtcc gctttgaagt aatcgcaccc
     5521 gcgaaactcg aggtcgccga ctgcaactgc tcgatctgca acaaatccgg gtatttgcat
     5581 ttgatcgtgc cggccgatcg cttcaagctc atcagcggcc gtgatgcgct cacgacctat
     5641 tcgttcaaca cgcatgttgc caagcatttc ttctgctcaa cctgcggcgt caagtccttc
     5701 tacatccctc gttcgcaccc cgacggggtc agcgtcaatg cgcgctgcat cgacagcggc
     5761 accgtcgaat cgatgtcggt gcaggcgttt aacggccgca attgggagca gggacgcgcc
     5821 gactatcgcg agccgcggag tcttgagtgc agcgcaaata aaacataagg cgccgcccga
     5881 ccttgattat gtccgcttga gctgaagtac ggcttctgcg gggcgaaatc tgtgacggac
     5941 atcgcgcgat gcttcctgcc ggttggggga agctatgcga gaccacagat cgcaatgaag
     6001 tatccgtcag ccatgcgtga gtcaattgca tccctggatg cgccgccgca ggatcgcgcg
     6061 gcacacgacg tcggcattcg agacagaatg ctgctcggat tggtgctgtt catcacctcg
     6121 gtgagcagcg aagcgctcgc cggcgatgcg ctatcgatca gcaattcggt tccggccgcc
     6181 gccgacttgc gcgctgcgac gccggccgcc ggtccgaggc tggaattctt taccgtacca
     6241 gcggtgggcg aggcacaaac attctcgagc acggactttc gtccgcgcag gccaaccttg
     6301 ttcgatagcg atccggcggc gaacgcctac ggcaacacgc caatgcttcg cggcacgacc
     6361 gtttggcagc ggctatcgga ttataaatcg cacgacggag tgcgcttatt gacgttgtgg
     6421 gaaacgccag ccagctcgat ttctctgcag gcggggaaac gcggcgaccc gtcgctgcaa
     6481 tggaccagcc ggctgatgaa tcacggcggc tcaacgcaag ggttgctcga tcggtggttt
     6541 tccgtatcgc tcaccggcgc gggcaatcgt ttacgtagct tgacgcggac gaccggcaca
     6601 cccgcgccgg ccaagcagac cgacaatccg gtggttgcgg gcctcaaata atcgaatcgt
     6661 cagcgcggcg ttgtgccgtt ttgggaacgt tcacgccaat agcagcgcgc atcgcgaacg
     6721 cgcgcgatag actattgcgc ggccactcgg gcggggcgcc cgtggtgcga ttcttcggaa
     6781 ttaacacaaa acctagggga aaaatctatg acactcacca agcacatggt cgcgctcgcg
     6841 gcttttgccg gtcttgcggc gacgagcctc acggcggtag cggacgagca cccattcacc
     6901 gaaggtcagg tcgtgcaggt atccgcaatt agaaccgaat acggaaagtt cgacgactac
     6961 atgaagttct tggacacgac ctggaaggca acggaagaag cggcgaaaaa agccggttac
     7021 atcaccggct acaaagtgat cacggtagag gcgcgcggtg agaacgatcc ggatatctac
     7081 ctcgtagtct atttcaagaa ctgggctgcg ttggacggtg cgaccgccaa ggccgatgcc
     7141 atctcgaccc aagtcgaagg gtcagtcgcg gctgccaaca agggcgcggt cgatcgcggc
     7201 aagattcgca gaatattggg atcctggacc ggacagcagc tcgacttgaa ataagtagta
     7261 gtgttttcga aaagccgggc cttcggcccg gctttctcac ggcaacactg gaccgatgac
     7321 ggtgttacac cggcgctcta gcgaatgcgt tgactgaaac tcacgccgta ggccgctggc
     7381 cgggcgagca gcaccgggtc ttgcgagggt tcgataccgg ccaatagatt caacgggctg
     7441 aacatgatca atttctgcgc ctgaacttga tcggcgaccg tcttcgtcag cgtgaggatg
     7501 cccagagaga cgagttcgcg gtccgccggc cacgtgatcg tcggatcatt gattgaatcg
     7561 tccttccccg cgacttgcgc caagaggcga aatttgaccg gccccttggc aaggcgcgtc
     7621 ggcaattcat ccatcaagta attcgggtcg gctttcttgg ccgcttcgtc gctcaaggcc
     7681 ggctcgtttg ccaccgggat gatctggtag cgcgcgtagt ggctcacgcc cttcgcgttg
     7741 gtgaacttga acgaattgac gccataaaag gccagcgtcg caaaactcgc gggcgccgct
     7801 cggcgcgtgc tcacgaattt ggcagccgcc ggatgtgtgt tcatgaagtt ctcgatcgga
     7861 gtgggtttcg gtacgtccgg tccgctgctg ccggccgcgc gcagcagcgc cagaaaatcc
     7921 tcaggagtcg ccaccggaaa tccattcgcc gaaatgctca cgatgtccgt gaatgcaccg
     7981 tttggcaagg tgaagcggat ggcgatgccg tgcggactcg ccgccggatc tgcgtcgggg
     8041 atggtgggaa cgccggtggt gtcggaaaac cggaccaaca ccggcacgcc tttctcaaaa
     8101 tgggtcgcct tgctgaacgc ggcggcgctc gggctcggcg tgaatcggcc ctcgaccatg
     8161 aggcccttgg catgattcgc gcgatatccg ggatggggtc cattggataa cttgttcaat
     8221 gcgtcgacca gcgcaatcgg cagcggctga ttagcggacg gcgcatcggc ccacgccggg
     8281 ccgactgagt agatcgccga cagcaaggcg acggacgaga cgacccgaac ccaattcatg
     8341 actcctccat tcttcagttg accgccaagt tagtagcgtt gcgcgtcgct ggccggtgcc
     8401 agcggattga tcggcgcttc ggttccgttg atcgtggtcc cgctgctcga gaaaacggcg
     8461 gcccgctgga cgaagtcatg gggaaaattg agcgcgggcg tgctgagctc atccagcgat
     8521 ttggactgcg caggcgcgag tttcaattcc agggccgcga ggttgtcctc gagttgctga
     8581 agcgtgcgcg caccgacgat cgtcgaggtc acaccgggac gattctgcac ccaagacagc
     8641 gcaacgcgcg ctggggtgga gcccgcctcg cgcgcgatcc cggccagggc atcgatcagg
     8701 gcgtagctct tctcattcag cgcggacatt gcccattcgc cgcgccccgc cttcacttgg
     8761 cccgcgtttt cgcgagtata tttgccgctc agcacgccgg acttcagcgg cgaccacggc
     8821 gtgatgccga gaccatgctc gagtgccatc ggcacgagct cgccttcgat ggtgcgctcg
     8881 agcagcgaat actcgagctg cagggcgatc agcggcgacc agccgcgcaa acgcgccagc
     8941 atctgcgctt ccatgacctt ccaggcgggc gtgtccgaaa aaccgagata gcgcaccttg
     9001 ccggcagcca ccaggtcgtc gagcgcgcgc atggtctcct cgatcggcgt gtgcacgtcc
     9061 caacagtgca tccagtacaa atcgatgtaa tcggtctgca gccggcgcag cgactcgtcg
     9121 cacgcggcga tgagcgcctt gcggccggct cctcccgaat taggatcccc gttcagcatc
     9181 gtgccgaaga acttggtggc gatcaccacg cgttggcgct ttgcgcgcgc gcctcgcagg
     9241 tgatcgccga gaatcttctc cgaatgtccg ttggtgtaga cgtttgccgt atcgatgaag
     9301 ttgccgccaa gatcgacgta gcggtcaaaa atgcgacccg agtcttccac gttcgagccc
     9361 cagccccaat cctcgccgaa ggtcatcgtg ccaaggcaga acggactcac ccgcaatccg
     9421 gaacgtccta gcgtcacgta atggctgagg ggcataattt ggttccgctt gaaatggagc
     9481 gacattttag catcggccgg gccgcagcgc ccgggtggga aggcagccca tcgttgggct
     9541 tataaagtgc gcccggcggg attactcggc gctacgcgcc tcgccccttc ggggtcgccc
     9601 tacgggcgat cagcgccact gcgtggcgct gtcgaacccg agttttttgt ggtgggttcg
     9661 gctctctaat gcgtcccgcc cgtcgtgggg attgtggatt tcttgcttat aaagtgcgcc
     9721 cggcgggatt cgaacccacg accctcggct tcggaggccg atactctatc cagctgagct
     9781 acaggcgcaa tgtacggggt ggcgatacta ctcgccgccg gcaatcggcg tccagcgcgc
     9841 cttggtttcg cagcagcgcg agctcgtgcc aatgctgttc gttccagaac cggcgtgcag
     9901 ctccggatgg cgatggcaag acaaatagtc gggtggtttc gaccatgtct tgcaacagcc
     9961 gccggacccg cgtagtaagc ggcgcggcgg gcggaagcgt tgccggccgc cgtcccacaa
    10021 aaaatgatat cgaggtggtg cgcaacaacg tcggggagaa tatggcggta attacagtaa
    10081 cctgcgctga gaatccacat gtgtccccga gggcgcgttg cagcgagagc cgcagcttgc
    10141 gtagatcgcg catggcgata cgcttggcgc ggccgacatt gatgccgcgg aacagcgcga
    10201 tgcactcggt catgcgcaga ttctcaaagg ttacaagcca gtctccgccg gaccgttcaa
    10261 gccgtcgaag aaatcgacga cgccggtttc caacgaatac tcggcgccca ccaccaaaag
    10321 accagccttc tgaatgaggt gttcgatgat atccgagccg tgccgcagat gtttcaccga
    10381 ggctcgaatg ttggcgcgaa ccgcggcgcg acccaaggcc ggccaatcgt gtctcagttc
    10441 agtcgccagc agccccacga cggacggccg tatgagatcc acaatcgagt gtaaattgtg
    10501 ggattgattc tccaccggcc gctgcaactg ccgcagagtc gtttcgatgg cgccgcagcc
    10561 tgtgtgaccc aagaccacca ccagccgggt accaaattgc tccgcagcaa attcgacact
    10621 gccgatctgc gaaggcgcga cgatgttgcc ggcgacgcga atgacgaaaa ggtcgcccaa
    10681 gccttgatcg aagacgattt cgaccggcac ccgagaatcg gaacagccga gaatgactgc
    10741 gaaaggttcc tgactcgcgg ccaattcaag ccggcgcacg cggcctgtaa ttgcgtcgcg
    10801 atttctgtcg tcggacgcga agcggcgatt gccgtcttgc aagagcgcga gcgcgtcgcc
    10861 ggccgatatc atcgatgctc cacttggttc ggctgccgcc ctcgcctatg cgctacgagg
    10921 gatggcccga ggaacgatcg cgatagccga ataggcgcgc gggcaagaac agaattgccc
    10981 gcaacagcgc gaacatggcg tcgaaggtga tcccgatgag gcgaaatggc agggacagaa
    11041 gccacagcag cggccagaga atgatggcga gcaatgccag cggccagcac aacaccaaga
    11101 gaaggcaaaa accgatgatc gcaagcaagg atttcacaaa attctctccg ccattccagg
    11161 gcacaacatt aacacacacc tcggcgccgc gtttactggc tccgtaaccg gccttcgagc
    11221 ggtgctttag cgtttgcgct tggccgcagg tgcggtcgcg gactcgacga tcctcgacag
    11281 ggtcttaggt gccgccaggc aatagctgag ttccaccaga ttacggactt ctttccagtc
    11341 gaccgccttg cggtccagcc gcaaaccgaa ccagccacga ggtccaatgt aggccggaag
    11401 ataaaatcgg cgcggctcgc gcgaggcgcg atcgatgttt tcgccgagcg cggctttgca
    11461 gcacacggaa acaataccat cgccgtggtg gttgttcaaa aaataggcaa acactttgcc
    11521 gcgcacgcga aaatccgcgt ggtcgccgag cgtacggcgt ccgacttccg gcaactcgag
    11581 acaaattttc gcgagcttcg tgagacgagg gtcttccttc gggtcgctca cgtcagcgtc
    11641 cttgcttgac atactcaatg cgtctgaagc gcaacgcaga ccagtcctcg tcaatcgcca
    11701 cctgccgcac cgtctcgaat ccggcattgc gcagcacatg ccaaccggtg tcgcggttga
    11761 actcgcaggc gtagcgcttc gatgtgccct tcgggtacgc gaaccacagc agcgcatcgc
    11821 cgacggcgcc cgcggcgaga attttcgaca agcgatccag ctccgcttgc cgcattgcga
    11881 aggccaacgc gaactcaacg gctctgggct tgttgggatc gcgaagtatt tcgacgtcct
    11941 tgagcttcga cagctcggtc tcgaaggcag agggcacatt gaagacggca atttcgcgtt
    12001 gcgctttcaa attgagtttt tgaaatatcg gcgtcatggt gaatggcccc gcgtgtgaac
    12061 ctgctcagtg atctcgcgcg agcgcaagcc tgagtccgac gacgatgaaa atgctgccga
    12121 cacagcggtt gaaccaactt ctaaatcgat ctccggttgc aagcttgctg ctgatccgcg
    12181 ccgtggacca ggcgaccaac aaattccaga tcgtaccgtt gaggtcgaag accgcaccca
    12241 ggaataagaa cggcaccggc ttggacggtg catccgacag cacgaactgc ggcagaaacg
    12301 ccaagaaaaa cagcgccact ttgggattca agacattggt gagaaagcct tggaaaaaga
    12361 tgccgcgcat tgcggcgggc cggcgatcgg cggcgcttgc cttagattga gtcgtgctgt
    12421 tcgagcgcag caatgaaata ccgacgtaga cgagataggc ggcgccgatt agtttgactg
    12481 cagcaaacgc ggtggcggac gcggccaata tagcggacaa acccaacgcg gctgcgcaga
    12541 catgcaccaa cacaccggtg ccgacgccaa gcgcggccac cgcgcccgct tgccaaccat
    12601 gggtcgagct gcggccgacg atatagagcg tgtcgggccc cggcgtgatg ttcaacagca
    12661 gcccggaaag cacgaatagc cagagatcat gggtgcctaa cacgttgcct cccggctggt
    12721 ccggtcgtca gcccttgcgt ggaaatttcg ccatcaacgc cgcgcgacgt tccaggcttg
    12781 cagccggcca gcgctcggcg gagcgatagt actcggcttc tgccgggata ttcggcgcaa
    12841 gcgtgaccca gggttgcttt gatgaggtga agatatggat gtccggcgga aagcccgcgg
    12901 gatgatcgag cgatcctacg cgaacgaagc gcagggcgtc tcccgcgccc gcatagttgc
    12961 tccacacggc gatttggcat ttggggcagc gtgagattcg ctggccttgg ccgctcttgg
    13021 aaggcgtgtc aatggcggcg acttcgccct cgaggagttg gatacgatcc gcttcgatca
    13081 acgcattgag cgcgaatgcg gcgcccgtct cccgctggca ccagctgcaa tggcaacagt
    13141 ggacgaacaa cggtttgccg atcatgcgat agcgaacgta acggcaggtg cagccgccat
    13201 catgagaact cgtcgcgccg ggagtgctca tcggcggcgc ctcgcggtcg cctcaatttc
    13261 gatttgcata cgcggatcgg acaatccggc ggacatcatg gtcgccgccg gacgaatcgt
    13321 gccgaagtag ttgcgcaaga tcggccaaca ctcgggaaag cgcgcggcgt cggggaggat
    13381 gtaggtgacc cgcacaacgt cggccaactc ggcgccggcc tgcctcaagg ccgcctgaat
    13441 gttcttcaga cactgctcgg tctggtcgct taatttgtcg gaaatcttca tggtcgcgta
    13501 atcgaagccg gtggtgccgg atacgaatat ccatttgccg tcgaccaccg ctcgagaata
    13561 gccaatgtcc tgttcaaatt gcgagcctga actgatcaac tggcggctca ttcaatgctc
    13621 ctcggataat gctgtcgagt ctgacccgcg ctctgccacg ccagtcggtg ctccagccac
    13681 acctcggcgg tgggcgggaa caggcttggg ttgtccatcg atgcggtggt gacatcgatg
    13741 ctcgtcgctc tgccggaagt ctcgtagaag agttggctgc cgcatatacc gcagaactga
    13801 cgaacgactc cctgcgacga tcgatagatg tgtggatggc ccgacaatat ctcgagccgc
    13861 gcgcgctcga acgtcaacca cgcaaccgat gttgcggcgc ttgctctgcg gcacgtcacg
    13921 cagtgacaaa tgaccgagaa ctcgggctct ccggaaacgc gaaaccgaat ggctgaacag
    13981 aggcagccgc cttgactgat ttgcatggtt ccccgccccc aacgtggtcg tcaacggccg
    14041 tcttggatcc gcagcgatat tatcaagtcg gcgcggctgg gaacaccgcg gagcgcggcg
    14101 cgctggcgaa gcaccggact atggcttgcg gcccgcgtat attgagctgc catggctgag
    14161 ccgcttcatg tgcaatgctg tatcgcgggc ggcggcccgg ccggcatgat gctcggctac
    14221 ctgctcgggc gggcgggagt caaaaccgtc gttctggaaa aacatgcgg
//
LOCUS       DASWZT010000355         6694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_2829, whole genome shotgun sequence.
ACCESSION   DASWZT010000355 DASWZT010000000
VERSION     DASWZT010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6694
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2829"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="VGE92_09925"
     CDS             <1..1113
                     /locus_tag="VGE92_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1314185.1"
                     /translation="DLSLGNYQPLGAPIDPVADQSDDVIASGYRAQNVALFGELEGDA
                     ATDLRWSVGLRGERWSATYHGITTDFLGTNTGYGNAPVLPTAIESVTPANLSPANNLW
                     GGHASLTFKLDPNESIYATASRGYKSGGFNLSQGLLPNQLSFNPETDLNGEAGYKAEL
                     LDRRLKVNADIFYLYRHDAQIKTSFQSDPTNPDDFVFYTGNAASGRNYGLESDVEWRT
                     SDRLTLGADLGLLQTHFEDFVQQGASGTALLSVSRELANAPHWQAAVDATYRDPRGPF
                     ARLDVTGMGGYYFDLPPNETTSRPYGLLNAKIGWETRRWSAYLSGRNLLDKRYPVRGF
                     YFGDVPPNFPNELYIQLGDPRTWLASVSVNFGGERR"
     gene            1199..3802
                     /locus_tag="VGE92_09930"
     CDS             1199..3802
                     /locus_tag="VGE92_09930"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY1314186.1"
                     /translation="MDAISGPYGRTLSEHADRVVDLREARPDAAGADAFLVGRRVGPY
                     VIDAEIGHGGLESVWRAHRADGRFEGTVAIKFLRWPDGIGERRFRTEGRLLGRLTHPN
                     IARLLDAGVLDAAQPYVVVEYVDGEPIDVYCERHALDVKARVQLFLSVLAAVAHAHSR
                     LIVHRDLKPANIFITVDGTVKILDFGIAKLLASTADDRPLTQSSLSPVTLQYATPEQL
                     LGQAVTTGTDVYALGLVLYVLLTGGHPAKAEACSSADFIRSVVAEDAPAASSVARLPS
                     IGARLLEGDLDNILAKALKKSPGERYVSAAAFAEDLKRYLNHQPVQASPDSLVYRAGK
                     FLRRRRAGVAVAAVLAVTILVAALSVYGQKVAADRERRQAQLEAARAQESYRFLSSLL
                     QEIGAEGGALSPTQIVDRGMYLLDHQTGLDPRSRVDELQMLATFYAGLYEAQKERSVL
                     ARAEELARRIAYPEGLVSVLCDEVDTELDRNDRDKAQARFAEARQLLGGLGRPPVRQQ
                     AQVEAAAASIAAANHDDPAAVADAERALEILRAGGQINTALYPTVLSRLSVYHDSLGH
                     AEQAHRYTELTAAAWDAVAGSGGIESLTALNNESVDLVNFGEVRAALATSAEVLRRLQ
                     ERGAGAAVQVPFRANYGARLAAIGRYAEALSFLDQAILDAGASHNQFWQLRAQFFRAC
                     ALVHAGDAGATAALDEIESAYRADAVKNAASLQLITVCRSEWLLSDGDAEAAHEVIQA
                     LLKAVDYPAHTAVPVLRTALPAAAHIALARHELSAAESYASAAADYARMQARDPNQSA
                     DLGRALTLLARTQHVGGNDADAVQSVQKALPSLAGGLGPEHAEVAAAQALLAEWSQGH
                     AQAIRSGRSSD"
     gene            complement(4391..4630)
                     /locus_tag="VGE92_09935"
     CDS             complement(4391..4630)
                     /locus_tag="VGE92_09935"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEY1314187.1"
                     /translation="MPTEFEEPAENATELTRLSEALDELAIREARLAELVDLKYFCGF
                     SFEEIAAQRASSVRTDQRDWEKARLILFNGLNDAT"
     gene            4660..4872
                     /locus_tag="VGE92_09940"
     CDS             4660..4872
                     /locus_tag="VGE92_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314188.1"
                     /translation="MFAPVDGANCPNARPGYARDHSLLLNHGLIRIFLQLPAKAEYTV
                     TVVHDPYGCALVVAPSSPRGHACERH"
     gene            5117..>6694
                     /locus_tag="VGE92_09945"
     CDS             5117..>6694
                     /locus_tag="VGE92_09945"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY1314189.1"
                     /translation="MIRCVALDVGLWFSLPCAFLLVYVLGYAEPTAAIAPHLGLVSLP
                     LLAIFLARLVLARSSASHRVYFSVTSTLTSSVLALFVIYYMAVLIGLHSWGGVVAWNV
                     VPTFFLQTSLITEVWKIPRVCIIAVPILVYWWHIALCRRYFDRVDWVPFLNPRLSGLM
                     FSSVLLVGLAIIVIVSVEFCTGAFTSQQEPLSVTAFPQSAGMDLEGYIVNPVTANRMD
                     LREDAARQAYVGADVFGKGVHRKNLVFILVDALRPDHMGLYGYDRDTTPVLSRIVREH
                     SSRIVQEVHSTCADTACSIFSLFSSQFPGHFAAHPFMLHHVLWRNDYRIHLILSGDKT
                     YFYSRKQYYGDVDTFYDGTQAPGYYMNDDQLVIDKLASMPPADQNPVMFHFHLMSAHI
                     LGKRHDGRGPFQPEQRYLFPDNHDTGPGGQESPGPRNFYDNGVVRADAVIGELLALLK
                     TKGYLEDALVVITADHGESLGEHGLYHHANSVREEVLRVPLVMIGYGRPLQLPATTRT
                     FASQVDIAPTILRELHLP"
BASE COUNT         1192 a         2075 c         2122 g         1305 t
ORIGIN      
        1 gacttgagcc ttggcaacta tcaaccgctg ggcgccccca tcgatccggt cgccgaccaa
       61 agcgacgatg tcatcgcaag cggctaccgg gcgcaaaatg tcgcgctgtt cggcgaactg
      121 gagggtgatg ccgcgacgga cctgcgctgg tccgtgggcc tgcgcggcga acgctggagc
      181 gccacgtatc acggaatcac caccgatttt ctcggcacca ataccggcta tggcaacgcg
      241 cccgtgctgc ccaccgccat cgagtcggtg acaccggcaa acttgagtcc ggccaacaat
      301 ttatggggag gtcacgcctc actgaccttc aagctcgacc cgaacgagtc gatctatgca
      361 acggcgtcgc gcggctacaa gtcgggtggc ttcaatttga gtcagggact gttgccgaat
      421 cagctgtcgt tcaatccgga aacggactta aatggcgaag cgggatataa ggccgagctg
      481 ctcgatcgtc ggctcaaagt gaacgcggac attttttatc tgtaccggca cgatgcgcaa
      541 atcaagacca gcttccaaag cgatccgacc aacccggacg atttcgtttt ctacaccggc
      601 aatgcggcga gcgggcgcaa ctacggcctc gagagcgatg tggagtggcg gacaagcgat
      661 cggctgacgt tgggcgcgga cttgggcctc ctgcaaacgc acttcgagga tttcgtacaa
      721 caaggcgcct ccggcaccgc gctgctctcc gtttcgcgcg agcttgcgaa tgcaccgcac
      781 tggcaggcgg cggtggatgc aacttatcgc gacccgcgcg ggccgtttgc gcggctcgac
      841 gtcaccggca tgggcgggta ttacttcgac cttccgccga atgaaacgac gtcgcgtccg
      901 tacgggctgc tcaacgcgaa gatcggttgg gagacgcgcc gctggtcggc ctacctctcc
      961 ggccgcaatc tcctggataa gcgctatccg gtgcgtgggt tttattttgg cgacgttccg
     1021 ccgaacttcc cgaacgagct ttatatccag ttgggcgatc cacggacctg gctcgcctct
     1081 gtttccgtga atttcggcgg cgaacggcgc tgacttgccg gcgacacaca actgtcataa
     1141 ctggccggcg cgcaaggagc gttgtgcgcg ccgcgccttg cggtatgctt tggcaacgat
     1201 ggacgctatc tccggtccct atgggcgaac actgagcgag cacgccgacc gggtcgtgga
     1261 tctgcgcgag gcacggccgg atgctgcggg cgccgacgca ttcctggtcg gacgacgggt
     1321 ggggccgtac gtcatcgacg ccgaaatcgg gcatggcggc ttggaaagtg tctggcgggc
     1381 gcaccgcgcc gatggccgct tcgaaggcac ggtcgccatc aaattcttgc ggtggcccga
     1441 tgggatcggc gagcgccgat ttcgcaccga aggccgcctg ctcggacgac tgacccaccc
     1501 gaacattgcg cgccttctcg acgccggtgt gctcgatgcg gctcaaccct acgtcgtcgt
     1561 cgagtatgtc gacggtgagc cgatcgacgt ctattgcgag cggcacgcac tcgatgtcaa
     1621 ggcgcgtgtg cagctgtttc tctcggtact cgccgccgta gcgcatgcgc acagccgtct
     1681 gatcgttcac cgggacttaa agcccgccaa catctttatc acggtcgatg gcacggtcaa
     1741 aatcttggat ttcggcatcg ccaagcttct cgcaagtacg gcggacgatc ggccattgac
     1801 tcaatcgagc ctgtcccccg tgacgctgca gtacgccacg cccgagcaac tcctgggcca
     1861 ggcggtcacg accggcaccg acgtctacgc gctgggactc gtgttgtacg tgctgctcac
     1921 gggcggccat cccgcgaaag ccgaggcgtg ctcgagcgcc gacttcatac gaagcgtggt
     1981 cgccgaagat gcccctgccg cctcgtcggt ggcgagactg ccgagcattg gtgcgcggct
     2041 gttggagggc gacctcgaca atatcctcgc caaggcgctg aagaaatcac cgggcgagag
     2101 gtacgtctcg gccgccgcgt tcgccgagga tctcaagcgc tatctgaacc accagcccgt
     2161 gcaggccagt cccgattcgc tcgtgtatcg ggcgggcaag ttcctgcgtc gacgtcgggc
     2221 gggcgtcgcg gtggccgccg tgctcgccgt gaccatcctg gttgcggcgc tgagcgtcta
     2281 tggacaaaaa gtggcggcgg accgcgagcg gcggcaggcg cagctcgagg ccgcgcgggc
     2341 gcaagagtcg tatcggttct tgagcagcct gttgcaggag atcggcgctg aaggcggcgc
     2401 cttgtcgccc actcagatcg tggaccgcgg catgtatctg ctcgatcatc agaccgggct
     2461 tgatccgcgc tcacgcgtcg atgagctgca gatgctggcc acattctacg cgggcttgta
     2521 cgaggcgcaa aaggagcgct cggtactcgc gcgcgccgag gagctggcga ggcgcattgc
     2581 ctaccctgaa ggcctcgtct ccgtgctctg cgatgaggtc gacaccgagc tggatcgaaa
     2641 cgaccgtgac aaggcccagg cacgattcgc cgaagcacgg caactgctcg gcggcttggg
     2701 ccggccgccg gtgcgccagc aggcgcaagt cgaagcggca gccgctagta tcgctgccgc
     2761 caatcatgac gacccggcag ccgtcgctga cgccgagcgg gcgcttgaaa tactacgcgc
     2821 gggcggtcag atcaacacgg cgctgtatcc caccgtcttg agccgactgt cggtgtacca
     2881 cgattctctc gggcatgccg agcaggctca tcgctatacg gagttgaccg ctgctgcctg
     2941 ggacgcggtg gccggcagtg gcggcattga aagcctcacc gcgctcaaca acgaatccgt
     3001 cgatctcgtc aacttcggtg aagtgcgagc ggcgcttgcg accagcgcag aggtgttgcg
     3061 ccgcctgcag gagcgcggcg cgggggccgc cgtgcaggtg ccgtttcggg ccaactacgg
     3121 cgcgcggctg gccgcgatcg ggcgctatgc cgaggcgctc agctttctgg accaggcgat
     3181 tctcgacgcc ggcgcgagcc acaatcagtt ctggcaactg cgcgcgcagt ttttccgcgc
     3241 ctgcgctttg gtgcatgccg gtgatgccgg ggcgacggcg gcgctcgatg agatcgagag
     3301 cgcctatcgc gcggatgcag tcaagaatgc agcttcgctg cagttgatca cggtgtgtcg
     3361 cagcgaatgg ttgctgagcg acggcgatgc ggaagcggcg cacgaggtca ttcaagcgct
     3421 gctcaaggcg gttgactatc cggcgcatac ggcggtgccg gtgttgcgca cggcgctgcc
     3481 cgcggcggca cacattgcgc tggcgcgcca tgagctgagc gccgccgaga gctacgcctc
     3541 cgccgcggcg gactacgccc gcatgcaggc tcgcgacccc aatcagagcg ccgatctggg
     3601 ccgcgcactc acgctgctcg cgcgcacgca acacgtgggc ggcaatgacg ctgacgcagt
     3661 gcagagcgtg caaaaggcac tgccctcact cgcgggcggc ctgggacccg agcatgccga
     3721 agtggcagct gcccaagcgc tgctcgcgga atggtcgcag gggcacgcgc aggcgatcag
     3781 atccggacgc tccagcgact aatttccgac atttggtact ccaattgtca aaattgcgcg
     3841 ctatggtgtc cgcagatgga gactctggac agagcccatt cggtggcgca ttttcagtca
     3901 atcgccgcag cgtatgtttc gacccgtgaa gtcgcggagc cgtgcccaca gccttctgga
     3961 gagtcgtctt gccaaaccac gcgaaacatc gggccgtcat gtcggctgcc atagaaaacg
     4021 aaattaggtt cttagaaggg gctcgcgatg cattgcccga agattcgcat ttgcgagcgc
     4081 tattcagcct tgcggcggag cgcttaggcg attttgtctc gcagcctaaa gcaaatgccc
     4141 cagcatcgca gggtaagaag cgccgtggca gcggtcagga acccgcctcg cagatcgggt
     4201 tcgtaacgtc caataagaag ggccggctcg ccgggaccat taggcgatcc tcgatccgag
     4261 ctatgtaacg aacggtcgtt tcggtcgtca cgcgcgccga gatcgatctt gccgatcgat
     4321 gtcgcctttc atcgcgatat ctcgatttat ccaatgaccg gcgcgagcct cgatggttgg
     4381 gtacggcgac ctacgttgca tcgttcaacc cattgaagag aatcaggcgc gccttctccc
     4441 agtcgcgctg atcggtgcgc acggagctgg cgcgttgtgc ggcgatttcc tcgaaactga
     4501 agccgcagaa gtacttcagg tccaccaact cggcgagacg cgcctcgcga atcgcgagtt
     4561 catcgagcgc ctccgacaag cgcgtaagct cggtcgcgtt ctcggcgggc tcctcgaatt
     4621 cggtgggcaa ttgggtgatg tggtaagcgc cgccgcgctt tgtttgcccc ggtcgacggt
     4681 gcgaactgcc cgaacgcgcg cccgggctat gcgcgcgacc atagtctgct cttgaaccat
     4741 gggctcatcc gtatcttcct gcagttgccg gcgaaagccg agtacaccgt cacggtggtg
     4801 cacgacccct acggctgcgc gctggtggtg gcgccatcaa gcccccgcgg ccacgcctgc
     4861 gaacgacatt gagctgagcg tggatgcggc cggatcgctc acggccgcgc acccacattc
     4921 agcgcgaaat ccctgaattt cgtgcgtcca tggcgcggcg ctaacgggcg caggtcacgc
     4981 gtcgcgcatc gagtccgtgt cgagtgatag tcaggtcaca gtatcggaaa tatggaattg
     5041 aacgggcgcg gacggcccga ttaaatgcgc atattgttaa ctcgactatc cggagcttgc
     5101 catcggtgtt gcatggatga ttcggtgtgt cgcgctggac gtcggactgt ggttttccct
     5161 gccatgcgcg ttcctgctcg tgtacgtgct cgggtacgcg gagcccacgg ctgccatcgc
     5221 accgcacctc gggctggtaa gccttccgtt gttggcgatc ttcctcgcgc gcctggtgct
     5281 ggcgcgctcg tcggccagcc atcgagtcta tttctccgtc acctcgacac tgacatcgag
     5341 cgtgttggcg ctattcgtca tctactacat ggcggtgctg atcggcctgc attcctgggg
     5401 cggtgtggtc gcatggaacg tcgtgccgac gttctttctg caaacctccc tgatcaccga
     5461 agtctggaag attccgcgcg tgtgcatcat cgcggtcccg atcctcgttt actggtggca
     5521 catcgctctg tgtcggcgct atttcgaccg ggtcgactgg gtgccgttcc tcaacccgcg
     5581 gctctcgggc ctcatgttct catcggtgct gctcgtgggc ctcgccatca ttgtgatcgt
     5641 gagcgtggaa ttttgcacag gagcgttcac ctcgcagcag gagcccttga gcgtcaccgc
     5701 ctttccgcag tccgccggca tggatttgga aggatatatc gtgaatcccg tgaccgcgaa
     5761 tcggatggat ctgcgggagg acgcggcacg tcaagcgtat gtcggcgcgg atgtttttgg
     5821 caaaggggtg catcgcaaaa acctggtgtt cattctggtc gacgcgctgc ggccggatca
     5881 tatgggattg tacggctatg accgcgatac gacgcccgta ttgagccgca tcgtacgcga
     5941 gcattcttcg cggatcgtgc aagaggttca ctcgacctgc gccgacaccg cgtgttccat
     6001 tttcagcttg ttcagctcgc aatttcccgg gcatttcgcg gcccatccat tcatgctgca
     6061 tcacgtgttg tggcgcaatg attatcgcat ccacttgata ttgagcggcg acaagaccta
     6121 cttttacagc cgcaagcaat actatggcga tgtcgatact ttctacgatg ggacgcaggc
     6181 cccgggttac tacatgaatg acgatcagct ggtgatcgac aagctcgcga gcatgccgcc
     6241 cgccgatcag aatccggtga tgtttcactt ccatctgatg tccgcccaca tcttgggcaa
     6301 gagacatgat ggacgcggtc cgttccagcc ggaacagcgc tacctctttc ccgacaacca
     6361 cgatacgggt cccggtgggc aagagtcgcc gggcccgcgc aacttttacg acaacggcgt
     6421 ggtgcgggcc gatgccgtca tcggggaact gctggccctg ctgaagacca agggttacct
     6481 cgaagacgcg ctggtcgtga tcaccgcgga tcatggcgag tcgctcggtg aacatgggct
     6541 ctatcatcac gccaacagcg tccgcgaaga agtgttgcgc gttcccttgg tgatgattgg
     6601 ctacggccgc ccgctgcagt tgccggcgac cacgcgcacg ttcgcatcgc aggtggacat
     6661 cgcgccgacg attctgcggg agttgcatct gccg
//
LOCUS       DASWZT010000356         2348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_56028, whole genome shotgun sequence.
ACCESSION   DASWZT010000356 DASWZT010000000
VERSION     DASWZT010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56028"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2253)
                     /locus_tag="VGE92_09950"
     CDS             complement(<1..2253)
                     /locus_tag="VGE92_09950"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY1314190.1"
                     /translation="MNALDSFHPAVAAWFSRTFDAPTPAQERAWPALQSGQHVLVAAP
                     TGSGKTFAAFLAAIDQLVKEGLQGALPDETRILYVSPLKALSNDIQRNLDAPLSGIRA
                     ELAALNLPEVAIRAVVRTGDTTQADRAGMRRKPPHIVVTTPESLYILLGSQSGRDMLA
                     TCRTVIVDEIHAIAGNKRGAHLALSLERLRALCGQSLTRIGLSATQRPIEEVAKFLTG
                     ATAADAGRCTIVDGGHARSRDLALELPPAPLEAVMSGEVWTLIYDRLAELVREHRTTL
                     VFVNTRRLVERVARHLSERLDKENVAAHHGSLSKERRLDAEQRLKRGELKVLVATASL
                     ELGIDIGDVDLVCQIGSTRSINAFLQRVGRAGHSVGGVSKGRLFPLSRDELVECAALL
                     DAVRRGELDRLHVPQNALDVLAQQITAEVAAREWSEDELYGLMRGAYPYRELRREEFD
                     ECVAMLAEGFSTRRGRRGALLHHDAVNKMLRARKGARLTALTSGGAIPDNADYKVMLE
                     PEGFLVGTVNEDFAVESLQGDIFQLGNTSYRILRVERGTVRVEDAHGQPPSIPFWLGE
                     APGRTEALSAAVSRFRLEIEQRLDQPGAASQWLTEVVGLDEVAAFQIVQYLSAARAAL
                     GCLPSFDTLVFERFFDESGGMQLVIHSPFGSRVNRAWGLSLRKRFCRTFNFELQAAAT
                     EDNIVLSLTHAHSFELADAARYLNSNSVRQVLVQALCAAPMFEVRWRWDAGISLALPR
                     FRGGKKIPPQI"
BASE COUNT          425 a          779 c          760 g          384 t
ORIGIN      
        1 gatttgcggc ggaattttct tgccgccgcg aaaacgcggc agcgccaacg agatgccggc
       61 gtcccagcgc cagcgcactt cgaacatcgg cgccgcgcac aacgcctgca ccagcacctg
      121 gcgcaccgaa ttcgagttca aatagcgcgc cgcgtcggcc agttcgaagc tgtgcgcgtg
      181 cgtcagcgac aggacgatgt tgtcttcggt cgccgcggcc tgcaactcga aattgaatgt
      241 gcggcagaag cgtttacgca gcgacagccc ccaggcgcgg ttcacgcggc tgccgaacgg
      301 ggaatggatg acgagctgca tgccgccgga ttcatcgaag aagcgctcga acaccagcgt
      361 atcgaagctc ggcaggcagc cgagcgcggc gcgtgcggcg ctcaagtatt gaacgatctg
      421 aaatgcagcc acttcgtcga gtcccacgac ttcggtcaac cattggctcg ccgcgccggg
      481 ctggtccaat cgctgttcga tttcgagtct gaaccgcgag accgcagcgg acagcgcttc
      541 ggtgcggccc ggcgcctcgc ccagccagaa cgggatggac ggcggctgac cgtgtgcgtc
      601 ctcgacgcgc accgtgccgc gctcgacccg caatatccga tacgaggtat taccgagctg
      661 gaatatatcg ccctgcaggc tttcgacggc gaaatcttcg ttgacggtgc ccaccagaaa
      721 tccttccggt tccaacatca ccttgtagtc ggcgttgtcg ggaatggcgc cgccggaggt
      781 cagagcggtc agccgcgcgc ccttgcgcgc gcgcagcatt ttgttgacgg cgtcgtgatg
      841 cagcagggcc ccgcggcggc cgcgacgggt gctgaagcct tccgccagca tggccacgca
      901 ctcgtcgaac tcctcgcgac gcagttcccg atagggatag gctccgcgca tcagcccata
      961 caactcgtcc tcggaccatt cgcgcgccgc gacctctgcg gtgatctgct gggcgagcac
     1021 gtcgagggca ttctgcggaa catgcaagcg gtccaactcg ccgcggcgca cggcatcgag
     1081 cagcgccgcg cactccacca attcatcgcg cgacaatggg aacaaacggc ccttggagac
     1141 accccccacg gaatgaccgg cgcggccgac tcgttgcaag aacgcattga tggagcgggt
     1201 cgagccgatc tgacagacga gatccacatc gccgatatcg atgccgagct cgagcgaggc
     1261 ggtcgcgacc aggactttca attcaccgcg cttcagacgc tgctccgcat cgagccgcct
     1321 ctccttggag aggctgccgt ggtgtgcggc gacattctcc ttgtcgagcc gctccgacag
     1381 atgccgcgcc acgcgctcaa ccagccgccg cgtgttcacg aacacgagcg tcgtgcgatg
     1441 ctcgcgcacg agttccgcca gccggtcgta gatcaaggtc cacacttcgc ccgacatcac
     1501 cgcttcgagc ggcgcggggg gcagttcgag cgcaaggtcg cggctgcgcg cgtggccgcc
     1561 gtcgacgatg gtgcagcggc cggcgtcggc cgcagtcgcg ccggttagaa acttcgccac
     1621 ttcttcgatg ggccgctgag ttgccgacaa gccgatgcgc gtcaaggact gaccgcacag
     1681 cgcccgcagc cgttccagcg acagcgccag atgggcgccg cgcttattgc cggcgatcgc
     1741 gtgtatttca tcgacgatga ccgtgcggca ggtcgcgagc atgtcgcggc cggactgtga
     1801 acccagcaag atatacagcg attcaggggt ggtgacgacg atgtgcggcg gcttgcgccg
     1861 catgcctgcg cgatcggcct gcgtggtgtc gccggtgcgc accaccgcgc gaatcgccac
     1921 ctcaggcagg ttgagcgcgg cgagttctgc gcggattccc gagagcggcg cgtcaagatt
     1981 gcgttgaatg tcgttcgaca gcgccttcag cggcgacaca tagaggatac gcgtctcatc
     2041 aggcagcgcg ccctgcagcc cttctttgac cagctggtcg atcgccgcga gaaacgcggc
     2101 aaaagtcttg cccgagcccg tgggcgcggc caccaacacg tgctgtccgg actgcaatgc
     2161 cggccacgcc cgctcctgcg ccggggtggg cgcatcgaag gtccgcgaaa accacgccgc
     2221 gacggcggga tggaacgagt cgagtgcgtt catggcatct gatcggcagg gtgaatctgc
     2281 agcataatcg ccattgacgc ggcttggcaa acgcaatccg tccggccgtg gcggcctgcg
     2341 aacggcgg
//
LOCUS       DASWZT010000357         3517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_75271, whole genome shotgun sequence.
ACCESSION   DASWZT010000357 DASWZT010000000
VERSION     DASWZT010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75271"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /locus_tag="VGE92_09955"
     CDS             <1..1309
                     /locus_tag="VGE92_09955"
                     /inference="COORDINATES: protein motif:HMM:NF025930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEY1314191.1"
                     /translation="AQATRIFDLMEKFAGYGFNKSHSAAYALLSYQTAWLKAHYPAAF
                     MAAVLSSDMDKTDKVVTLIDECAGMELTVHPPDVNESVYAFKVSGPMSIRFGLGAIKG
                     VGASAVEAIIEERGAHGPFDSLTDLCRRIDLQRVNRRVFDALIRSGSLDRIGPNRATL
                     TAELDRAMHAGEQNSRAMSVGQVDLFGLSAAPDSAVADWSEAQRLAGERETLGLFLSG
                     HPITPYEPDLKFLGVARLADVGGPKPAAPVEGMRSWSAGKPATVAGLVLEIRRRPNRV
                     TLILDDRSARLEVSLYEEVYQQHRDIIVKDAILIIDGTLRFDDFIEAWRLQAKSLMDI
                     DRARERFARRLWLKWPDEFDGPQGMNRFEQMLKPHLRGPCGVSVSVNRSDYTGRLNLA
                     DNWSVHASRTLLDKLTDVVGRDGWYLVYGPRNDVRGEETSSWQ"
     gene            1300..2259
                     /locus_tag="VGE92_09960"
     CDS             1300..2259
                     /locus_tag="VGE92_09960"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019692099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEY1314192.1"
                     /translation="MAMNFLEFEQPIAELEAKIEELKFLGADASVNINEEIKRLQSKS
                     RALTHSIFANLSPWQITQLARHPQRPYTLDYVSMIFTDWHELHGDRMYGDDLAIVGGL
                     ARIEAIPVMLIGQQKGRDTKERVRRNYGMPKPEGYRKALRLMKMAERFRIPVVTLIDT
                     PGAYPGIGSEERGQSEAIARNLFEMSNLAAPVLSVVIGEGGSGGALAIGVCDRLIMLQ
                     FSVYSVISPESCASILWKSTEKKEIAADAMGGTAERLKKLGLVDEVLKEPHGGAHRDP
                     QAMAETVKQSIVKNLAELCNQPVTQLLDARQARLREFGAFRDA"
     gene            2240..>3517
                     /gene="tilS"
                     /locus_tag="VGE92_09965"
     CDS             2240..>3517
                     /gene="tilS"
                     /locus_tag="VGE92_09965"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEY1314193.1"
                     /translation="MRSETHSASFSLRAVLDAHLPAGVTALVVGLSGGADSAALLVLA
                     AALGAEFRGRLRAVHIDHGLQSAAASFREACTAACTALAIPLSIIPVRLEPDGSSVEA
                     AARDARYAALEAQLRPGECLLTAHHREDQAETLLLQALRGAGLRGMSAMPICRPFGRG
                     WHLRPLLEVSQCELLKHGASLQGAYFLDPMNHDLRIDRSYLRQQIWPSLKARWPGAAN
                     ALSRSARHAAQAQLLLDRAAAVELARLRDGDALSLQGLRALDSQQQINVLRFWLNAAG
                     VEAPPTSRLLEALRQIFDADMDHLPCIAWGRHALRRYRQRIFLTASHPPQLLGTLEWP
                     RAAGSAVDLGPGLGALKWVSQNGGIDVPRLPATLSVRRRQGGETLKPAAQAKTTSVQH
                     LCQLQGVLPWMRDALPLVFADDVLVAVGDLWLDA"
BASE COUNT          636 a         1019 c         1172 g          690 t
ORIGIN      
        1 ggcacaggcc acgcgcattt tcgacttgat ggaaaaattc gcgggctacg gattcaataa
       61 gtcgcactcc gcggcgtacg cattgctgtc gtatcagact gcatggctca aagcgcatta
      121 ccccgccgca ttcatggcgg cggtgctgtc ttcggacatg gataaaaccg acaaggtggt
      181 cacgctgatc gatgaatgcg cgggcatgga actcaccgta catccgcccg atgtgaacga
      241 atcggtctac gcgtttaaag tgtcggggcc gatgagcatt cgctttgggc tgggcgccat
      301 caagggtgtg ggcgcatcgg ccgtcgaagc catcatcgag gagcgcggcg cccacgggcc
      361 cttcgatagt ctgacggact tatgccggcg catcgatctg cagagggtca atcgtcgtgt
      421 attcgacgcg ttgattcgct cgggaagtct cgatcggatc ggccccaatc gcgcgacctt
      481 gaccgctgag ctcgatcgcg cgatgcatgc gggcgaacag aattcgcgtg ccatgagcgt
      541 cgggcaggtg gatttgttcg gcttgagcgc ggcccccgac agcgccgtcg cggactggag
      601 cgaagcacag cgcctggcgg gcgagcgcga aacgctcggc ctgttcttga gcggacaccc
      661 gatcactccc tacgaacctg atttgaaatt cctgggcgtg gcgcggctcg ccgatgtcgg
      721 cggccccaaa cccgcggcgc ccgtcgaagg catgcgcagc tggtcggccg gcaagccggc
      781 caccgtcgca ggtttagtgc tcgagatccg ccgccgtccg aaccgcgtga cattgatcct
      841 tgacgatcgc agcgcgcgtc tcgaagtcag tctgtatgag gaggtttatc agcagcaccg
      901 cgacatcatc gtcaaggacg cgattctgat catcgacggc acgttgcgtt tcgacgattt
      961 catcgaagcg tggcgcttgc aggcgaagtc gctgatggat atcgaccgcg cccgcgagcg
     1021 ctttgcgcgc cgattatggc tcaagtggcc cgacgaattc gacggtccgc agggtatgaa
     1081 tcgattcgag cagatgctga aaccccatct gcgtggtccg tgcggcgtca gcgtgtcggt
     1141 caaccgctcc gactatactg ggcgcctgaa tttggctgac aactggtcgg tgcacgcatc
     1201 gcgaacgctg ctcgataagt taacggacgt ggtgggtcgc gacggttggt atttagtgta
     1261 tggtccgcgt aatgatgttc gcggcgagga aacgtcttca tggcaatgaa ttttctggaa
     1321 tttgagcagc caatcgcgga gctcgaggcg aagatcgagg agttgaaatt cctgggcgcg
     1381 gacgcgagcg tgaatatcaa cgaggaaatc aaacgcctgc aatcgaagag ccgggcgctc
     1441 acccatagca ttttcgccaa cctctcgcct tggcaaatca ctcaattggc gcgccatccg
     1501 cagcgtccgt atacgctcga ctatgtatcg atgattttca ccgattggca cgaactgcac
     1561 ggcgatcgaa tgtacggcga cgatctggca atcgtcggcg gcttggcgcg catcgaggca
     1621 ataccggtga tgctgatcgg tcagcagaag ggccgcgata ccaaggagcg ggtgcggcgc
     1681 aactatggca tgccaaaacc cgaaggctat cgcaaggcat tgcggctgat gaaaatggcg
     1741 gaacggttcc gcattccggt cgtcacgctg atcgatacgc cgggcgctta ccccggaatc
     1801 ggctccgaag agcgcggcca gagcgaggcc attgcacgca acttgttcga aatgtccaat
     1861 ttggcggcgc cggtattgag cgtggtcatc ggcgaaggtg gatcgggagg cgcgctcgcc
     1921 atcggcgtgt gcgatcggct gatcatgctg caattcagcg tgtattcggt tatttcccct
     1981 gaaagctgcg catccatttt gtggaagagc acggagaaaa aagagatcgc ggcggatgcc
     2041 atgggcggta cggcggagcg gctcaagaag ctgggtctgg tggacgaagt tctaaaggag
     2101 ccgcatggcg gggcgcatcg cgatccgcag gccatggcgg aaaccgtcaa gcaaagcatc
     2161 gtcaagaatt tggccgagct ctgcaatcaa ccggtgactc aattgcttga tgcgcggcag
     2221 gcgcggctgc gcgaattcgg tgcgttccga gacgcatagc gccagcttct ctctgcgcgc
     2281 ggtccttgac gcccatcttc cggcaggcgt caccgcattg gtggtgggct tgagcggcgg
     2341 tgcggattcg gccgcactgt tggtccttgc cgcggcactg ggcgccgaat ttcgcggtcg
     2401 cttgcgggcc gtgcacatcg atcacggctt gcagagcgcg gcggcgtcgt ttcgcgaagc
     2461 ctgtacggcg gcgtgcacgg cgctcgccat tcctctgtcg atcatacccg tgagattgga
     2521 gccggacgga agctcggtcg aggcggcggc gcgcgatgcg cgctatgccg cgctcgaggc
     2581 gcagctgcgt cccggcgagt gtctcttgac cgcgcatcat cgcgaggatc aggccgaaac
     2641 gctgctgctg caagccttgc gcggcgcagg cctcaggggc atgtcggcca tgcccatctg
     2701 ccgcccgttc ggccgcggtt ggcatttgcg gccgctgttg gaggtatcgc agtgcgagct
     2761 tctcaagcac ggcgcgagcc tgcaaggcgc ctattttctg gatccgatga accacgacct
     2821 cagaatcgat cgcagctatt tgcggcagca gatttggccg tcgctcaagg cgcgctggcc
     2881 gggcgcggcg aacgcactgt cgcgaagtgc gcgacacgcg gcgcaggcgc aactgctgtt
     2941 ggatcgcgcg gcggccgttg agctcgcgcg gctgcgcgac ggcgacgcgc tctcgttgca
     3001 aggcttgcga gcattggatt cgcagcagca gatcaatgtg ctgcgtttct ggctgaacgc
     3061 tgcgggtgtc gaggcgccgc cgacctcgcg gctgctcgag gcgctgcggc aaatcttcga
     3121 tgcagacatg gatcatttgc cttgcatcgc ctgggggcgc catgcgttgc ggcgctatcg
     3181 acagcggatt tttcttaccg cgagtcatcc gccgcagctt ctcggcacac ttgagtggcc
     3241 gcgagcggcc ggatccgcgg tcgatcttgg ccccgggttg ggagcattga agtgggtgtc
     3301 gcaaaatggc ggcatcgacg tgccgcgctt gcctgcgacc ttgtcggtgc ggcggcgcca
     3361 aggaggcgag acgctcaagc ccgcagcgca agccaagacc acgagcgtgc agcacttgtg
     3421 tcagctgcag ggcgtgctgc cgtggatgcg cgacgcgctg cccctggtgt ttgcggatga
     3481 cgttttggtt gccgtcggcg atctgtggtt ggacgcg
//
LOCUS       DASWZT010000358         6604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_60844, whole genome shotgun sequence.
ACCESSION   DASWZT010000358 DASWZT010000000
VERSION     DASWZT010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6604
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60844"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /gene="hpnH"
                     /locus_tag="VGE92_09970"
     CDS             complement(<1..606)
                     /gene="hpnH"
                     /locus_tag="VGE92_09970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HEY1314194.1"
                     /translation="MAIPLLQMYKVARYIAGRKLRGDKRYPLVLMLEPLFQCNLACSG
                     CGKIDYPKEILQKRVGVEDALRAVDECGAPIVSIPGGEPLIHKEMPQIVAGIVARGKF
                     VYLCTNAILLAKHIDEYQPSPYLTFSIHLDGNRERHDESVCQEGVFDKAVAAIQLAKS
                     KGFRVTVNCTLFNGEDPEGVAQFFDMATELGVEGITVSPGYS"
     gene            complement(615..1193)
                     /gene="idi"
                     /locus_tag="VGE92_09975"
     CDS             complement(615..1193)
                     /gene="idi"
                     /locus_tag="VGE92_09975"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF002995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEY1314195.1"
                     /translation="MNDFTEAARGSGADADSLILVDEADQGVGHMNKAECHAGRGILH
                     RAFSLLIFNGSGELLLQQRAASKRLWPLYWSNSCCSHPRSTETMEAAIERRLFEELGL
                     RCPLQFLFKFKYQAQFDATGAEHELCWVYIGRSTDSVNINCDEIHAWRWISPEALQVE
                     MADGGGNFTPWFMLEWARVWRDHRAQVLGLQW"
     gene            complement(1201..2205)
                     /gene="hpnA"
                     /locus_tag="VGE92_09980"
     CDS             complement(1201..2205)
                     /gene="hpnA"
                     /locus_tag="VGE92_09980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HEY1314196.1"
                     /translation="MRALVTGATGFVGAAVARALLTAGWQVRVLVRGSSDRSNLQHLA
                     VEAVEGDLAQVVSLERALAGCAGLFHVAADYRLGARDPKPLYRTNVEGTRNILNAART
                     AGVGRVVYTSSVATIGIPADGSPGNELTPVALGNMIGHYKRSKYLAEEVVMDAARMGM
                     SVVIVNPSTPVGPGDVKPTPTGQLVLDAACGRMPAYVDTGLNIVHVDDVAAGHLLAFE
                     RGRAGERYILGGEDMTLRTILDEIARLVRRPPPRIRLPYAAVLPMAYVAEGIAKITGR
                     SGRLTLEGVRMSRKRMFFSSSKAASELGYRWRPPREAFEDALRWFRGRGLLAPAQERL
                     "
     gene            complement(2202..2885)
                     /locus_tag="VGE92_09985"
     CDS             complement(2202..2885)
                     /locus_tag="VGE92_09985"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314197.1"
                     /translation="MGVVAALEAEARTLGPKVRRGDGLSTLSDGALLAVSGMGGHLAA
                     IAARTLVAAGAKSLMSFGLAGGLDPRLTAGSVVVPSEVISREGGRFITTQTWCEQFRM
                     ALGTRLDVASGRLLSSARPIDAVADKATVFRETGAVAVDMESVSIADVAAAHGLPFIA
                     VRVIVDTAADVLPSAVLAASRGGEVDVRRLIRGLVAKPTDLLPLLRLARRYRAATRSL
                     QAIAGARVA"
     gene            complement(2891..4873)
                     /gene="shc"
                     /locus_tag="VGE92_09990"
     CDS             complement(2891..4873)
                     /gene="shc"
                     /locus_tag="VGE92_09990"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEY1314198.1"
                     /translation="MSRIAPKPGSYPAPESDARGDIVSHSLRRVSDSPLDRAVADARD
                     ALIARQDAQGHWLFELEADCTIPAEYIMMMHFLDEIDAALEAKICRYLRSAQAEHGGW
                     PLYHGGELNISCTVKAYYALKLAGDSPDAPHMARARAAILKRGGAARCNVFTRIALAL
                     FGQLPWRGVPYIPVEIMLLPRWFPFHLDKVAYWSRTVMVPLFILCTRKPVAKNPRNIH
                     IRELFTIAPEEERGYFARKGAVAKIFLAIDRLGRLIDPLIPARTREKATRRAESWMLE
                     RLNGEDGLGAIFPAMVNALEAMVILGYPADDPRRVTAKHALQKLLVVSPSSAYCQPCV
                     SPIWDTALASLAMQEAGDGASSEASTRALDWLKTRQLLDEDGDWRVNRRGLAGGGWAF
                     QFANAYYPDLDDTAVVVWSMHQARDSSRYAENVRRALDWLVGMQSKDGGFAAFDADNT
                     SYHLNNIPFADHGALLDPPTSDVTARVATVLARIRRPQDEPALERALAYLRKQQEPNG
                     SWYGRWGTNYIYGTWSVLTALAQAHLGSDDPAVARAVEWLKSIQNPDGGWGESNDSYA
                     YPPCAHAKFPSTAYQTAWALLGLLAAGEARSEAVRLGAEFLMRTQQADGLWSDPSFTA
                     PGFPRVFYLRYHGYSAYFPLWALAAYRTLTRRGTAH"
     gene            complement(4870..5994)
                     /locus_tag="VGE92_09995"
     CDS             complement(4870..5994)
                     /locus_tag="VGE92_09995"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEY1314199.1"
                     /translation="MDSVSSDEVYQDRILPHVSRTFALTIPQLPLRLRTAVTNAYLLC
                     RIADTIEDEPALSPPETLSFLQRFSAALAGEGDAQLLAREIALRLSDRTLPAERDLVC
                     NMDRVLRVLSGLNAPQRAAIQRCVDLMCRGMPRFQFSASLQGLAQSSDLDEYCYYVAG
                     VVGEMLTDLFCDYSPQIARERPALCAIAASFAQGLQMTNILKDVWEDRSRGTCWLPQE
                     VFTRYGVDLGSVSSEPFDPRFAAGFRELVGVAHAHLRNALDYTLLIPSEETGIRRFCL
                     WAIGLAVLTLRKIEENPRFTAGAQVKVARSAVAMTRVLTNVAGRSSWMLRLLFSQAAR
                     GLPLAELADARPPRSVPVNSVATVDEPERALRRSYGNSGS"
     gene            6118..>6604
                     /locus_tag="VGE92_10000"
     CDS             6118..>6604
                     /locus_tag="VGE92_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEY1314200.1"
                     /translation="MSAPSVTYPLLESINAPEDLRRLPTTKLPGLAAELRQFLIQSVS
                     TRGGHFAAGLGTVELTIALHYVFNTPYDRLVWDVGHQAYPHKVLTGRRDRLHTIKQHQ
                     GLAPFPTRSESEFDTFGVGHSSTSISAALGMAVAAAQRGEERRAVAIIGDGALTAGMA
                     FE"
BASE COUNT         1255 a         2176 c         2056 g         1117 t
ORIGIN      
        1 gctgtacccc ggcgacaccg tgatcccctc gacgccgagc tcggtcgcca tgtcgaagaa
       61 ctgcgcgacg ccctccgggt cctcgccgtt gaacaaggtg cagttgacgg taacgcgaaa
      121 gcccttggac ttggccaatt gaattgccgc cactgccttg tcgaacaccc cctcctggca
      181 caccgactca tcgtggcgtt cgcgattgcc atccaggtga atcgagaaag tgaggtacgg
      241 cgagggctga tactcatcga tatgcttggc aagcaggatg gcgttggtgc acaaatagac
      301 gaatttgccg cgcgccacga tgccggcgac gatttgcggc atctccttgt ggatcagggg
      361 ctcgccgccc ggtatcgaca cgatgggcgc cccgcattcg tcgacggccc gcaatgcgtc
      421 ttcgacaccc acccgttttt gcagaatttc cttggggtaa tcgatcttgc cgcagcccga
      481 gcaagccaaa ttgcactgga atagaggttc cagcatgaga accaacggat accgtttatc
      541 accgcgcagc ttgcggccgg cgatataacg ggcgaccttg tacatttgaa ggagggggat
      601 agccatgagt catcttacca ctgcaaaccg agcacttggg cgcgatgatc ccgccaaacc
      661 cgcgcccatt cgagcataaa ccacggggta aagttcccgc cgccgtccgc catctccacc
      721 tgcagcgcct ccgggctgat ccagcgccat gcatggatct catcgcaatt gatgttgaca
      781 gaatcggtcg agcgcccgat gtaaacccag catagctcgt gctccgcgcc ggtcgcgtcg
      841 aattgcgcct ggtacttgaa tttgaacagg aattgcaggg gacagcgcag ccccaactcc
      901 tcgaaaagcc ggcgctcgat ggccgcctcc atggtctcgg tgctgcgcgg gtggctgcaa
      961 cagctattgg accaatacag gggccagagc cgtttggagg cggctcgttg ctgcagcagc
     1021 aattcgccgc tgccgttgaa tatgagcagc gagaacgccc tgtgcaggat gccgcggccc
     1081 gcgtgacact cggccttgtt catgtggccg acgccctgat cggcctcgtc caccaggatc
     1141 agggaatctg cgtccgcgcc gctgccgcgc gccgcctcgg taaaatcgtt catggccgga
     1201 tcatagccgc tcttgggcgg gggccaaaag cccgcggccg cggaaccagc gcagcgcatc
     1261 ctcgaacgcc tcgcggggcg ggcgccagcg gtaccccagt tcgctcgccg ccttgctgct
     1321 cgaaaagaac atccgcttgc gcgacatgcg tacgccctcg agcgtcaatc ggccgctgcg
     1381 tccggtgatt ttggcgatgc cctccgccac ataggccatg ggcaaaactg cggcgtacgg
     1441 tagacggata cgcggcggcg gccgccggac cagacgcgca atctcgtcga ggatggtgcg
     1501 caacgtcatg tcctcgccgc ccaaaatata gcgttcaccc gctcgcccgc gttcgaatgc
     1561 caataagtgc cctgcggcca catcatccac atgaacgata ttgagaccgg tatccacgta
     1621 tgccggcatc cggccgcagg ccgcatcgag gaccagttgc ccggtcggtg tgggcttgac
     1681 gtcgcccgga cctacgggtg tggacgggtt cacgatcacg accgacatgc ccatccgtgc
     1741 ggcatccatt accacctctt ccgccaggta ctttgagcgc ttgtagtggc cgatcatgtt
     1801 gccgagggcc accggcgtga gctcatttcc cggcgagccg tccgcgggaa tgccgatggt
     1861 cgcaacgctg gaggtgtaga cgacccggcc gacgccggcg gtgcgggcgg cgttcaagat
     1921 attgcgggtt ccttcgacat tggtgcggta caggggttta ggatcccgcg cgccgagtcg
     1981 atagtccgcg gcgacatgaa acaaacccgc gcaccctgcc agcgcgcgct cgagcgaaac
     2041 cacctgcgcg aggtcacctt cgacggcttc taccgcgaga tgctgcagat tgctgcggtc
     2101 cgagctgccg cgcaccagga ctcgcacttg ccacccggcc gtcaacagcg ccctggcaac
     2161 ggcggcgccc acgaaccccg tcgcccccgt gaccagcgcc ctcatgcgac gcgggcaccg
     2221 gcgatggcct gtaacgaacg agtcgcggcc cgatagcgcc gcgccaagcg cagcaagggc
     2281 aacagatccg tgggcttcgc gacgagcccg cggatgagtc gccgcacatc gacctcgccg
     2341 ccgcggctcg cggccagtac cgcgctcggc agtacgtccg cagcggtatc gacgatgacc
     2401 cgcacggcga taaagggcag accgtgggcg gcagcgacgt cggcgatcga cacgctttcc
     2461 atatcgaccg cgaccgcgcc ggtctcgcga aacacagtcg ccttgtccgc cacggcatcg
     2521 atcggccgcg cgctgctgag taaccttccc gaggccacgt cgagccgcgt gccgagggcc
     2581 atgcgaaact gctcgcacca cgtttgcgtg gtgatgaacc ggccgccctc ccgggaaatc
     2641 acttcgctcg gcaccaccac actgccggcg gtcaagcgcg gatcgaggcc gccggcgagt
     2701 ccgaaactca tcagggactt ggcgccggcc gcgaccagag tgcgggcggc gattgcggcg
     2761 aggtggcccc ccatgccgct gacggcaagc agcgcaccat cgctcaaggt cgacaagccg
     2821 tcgccgcgcc gcaccttggg tcctagggtg cgcgcctccg cttcgagcgc cgcgacgacg
     2881 cccaccccat tcaatgagcg gttccgcgac gcgtcagggt tcgataggcc gccaacgccc
     2941 agagagggaa gtacgcggaa tacccgtggt atctgagata gaacacgcgc ggaaatcccg
     3001 gcgccgtgaa actcggatca ctccacagtc catccgcctg ctgcgtgcgc atcaggaact
     3061 cggcacccaa ccgcaccgcc tccgatcgcg cctcgcccgc ggcgagcagg ccgagcagcg
     3121 cccaggcggt ctgataggcg gtactcggaa acttcgcgtg cgcgcagggc gggtaggcgt
     3181 agctgtcgtt gctctcgccc caacccccat cgggattttg aatgctcttc agccattcta
     3241 ctgcgcgagc aaccgccggg tcgtcgcttc ctagatgggc ttgcgccaac gcagtcagca
     3301 ccgaccacgt gccataaatg taattggtgc cccagcggcc ataccatgaa ccgttgggtt
     3361 cttgctgttt gcgcaggtag gcgagcgcgc gttccaacgc gggctcgtct tgcggacgcc
     3421 ggatgcgcgc cagaaccgtg gcgacccgcg cggtcacgtc gctggtcggg ggatcgagca
     3481 gagcgccgtg atcggcgaag ggaatattgt taaggtggta gctggtgttg tccgcatcga
     3541 acgcggcaaa gccgccatcc ttgctctgca tgccgaccag ccaatccaag gcacggcgta
     3601 cattctccgc gtagcggctc gaatcgcgcg cttggtgcat gctccagacg accacggccg
     3661 tatcgtcgag atcagggtag taggcattcg caaattgaaa cgcccatccg ccgccggcga
     3721 gcccccggcg attcacgcgc cagtcgccgt cctcatcgag caattgtcgg gttttcaacc
     3781 agtcgagcgc gcgcgtcgac gcctcgctcg acgcgccgtc accggcctct tgcatcgcga
     3841 gactggcgag cgccgtatcc caaatcggcg aaacacaggg ctggcagtaa gcgctcgacg
     3901 ggctcacgac cagcagcttt tgcaaggcgt gttttgcggt gactcgccgc gggtcgtccg
     3961 cgggataacc caagatcacc atcgcctcca aggcgttgac cattgccgga aagatggcac
     4021 ccaaaccgtc ttcgccgttg agccgctcga gcatccagga ttccgcgcgt cgcgtcgctt
     4081 tctcgcgggt acgcgccggg atcaaggggt caatcagccg cccgagacga tcgatcgcca
     4141 gaaaaatctt ggcgacggcg cccttgcgcg cgaaataacc gcgttcttcc tcgggcgcga
     4201 tggtgaatag ctcgcgaata tgaatatttc gcggattctt ggcgaccggc ttgcgcgtgc
     4261 acagaatgaa cagaggaacc atcacggtgc gcgaccaata ggccaccttg tcgagatgga
     4321 atggaaacca tcgcggcagc aacatgatct cgaccggaat gtagggtacg ccgcgccacg
     4381 gcagctgccc gaatagcgcg agcgcaatcc gcgtgaacac attgcaccgc gccgcgccgc
     4441 cgcgcttgag aatcgccgcg cgagcccgcg ccatgtgcgg ggcgtccggg ctgtcgcctg
     4501 caagcttcaa ggcgtagtag gctttgacgg tgcagcttat gttcaactcg ccgccgtgat
     4561 acagcggcca accgccgtgc tcggcctggg cggagcgcaa gtagcggcag atcttggcct
     4621 ccagcgcagc atcgatctcg tccaggaagt gcatcatcat gatgtactcg gccgggatgg
     4681 tgcagtccgc ttcgagctcg aacagccagt gtccctgcgc atcctgccgt gcgatcaacg
     4741 catcccgcgc atcggcaacc gcgcggtcca agggtgaatc ggacacccgg cgcagcgagt
     4801 gcgagacgat gtcgccgcgc gcgtcggact ctggtgccgg gtaagagccc ggcttcggtg
     4861 cgatacgact cacgatcctg agttcccgta agagcggcgc agggcccgtt caggctcatc
     4921 gaccgtcgct acggagttga cgggcacgga acgcggcggc cgcgcatcgg ccagctcggc
     4981 caggggcagg ccgcgtgctg cctgggagaa cagcagccgc agcatccaac tgctgcgtcc
     5041 cgcaacattg gtcaatacgc gggtcatggc caccgcggaa cgcgccactt tgacctgggc
     5101 accggcggta aagcgcggat tttcctcgat cttgcgcagg gtgagtaccg cgagcccgat
     5161 cgcccatagg cagaaacggc gaatacccgt ctcctcgctg gggatcagca gggtgtaatc
     5221 cagcgcgttg cgcaagtggg catgcgccac accgaccaac tcgcgaaagc ccgcggcaaa
     5281 gcgcgggtcg aagggttcgc tcgacacgct tccgagatcg acgccgtaac gggtgaatac
     5341 ttcctgcggc agccaacagg tgccgcgact gcgatcctcc cacacgtcct tgaggatatt
     5401 cgtcatctgc agcccttgcg caaaggaggc ggcgatcgcg cacaaggccg gccgctcgcg
     5461 ggcaatctgc ggcgaataat cgcagaatag gtcggtcaac atttcaccga ccacgccggc
     5521 gacgtagtag caatactcgt ccagatcgct cgactgggcg agaccttgca gactcgcgct
     5581 gaattggaac cgcggcattc cgcggcacat gaggtcgacg cagcgctgga ttgcggcccg
     5641 ttgcggcgcg ttcagacccg agagcaccct gaggacgcga tccatattgc agaccaggtc
     5701 gcgctccgcc ggcagggtgc ggtccgatag ccgcagcgcg atttcacggg ccagcagttg
     5761 ggcatccccc tcaccggcga gcgcagcgct aaagcgttgt aaaaatgaca atgtctcggg
     5821 cggcgagagt gccggctcgt cctcgatggt atcggcgatg cggcacagca aataggcgtt
     5881 ggtgaccgcc gtgcgcagcc gcaggggtaa ttgcggaata gtcagcgcaa acgtgcgcga
     5941 gacgtggggg agaatccggt cttgatagac ttcatctgaa ctgacggaat ccatgcggac
     6001 agtctctcgg aaaccctgaa gaatagcatc atgtcagcgg aatccccgtg gtgcaaattt
     6061 gcaacatcgg gtgcagtcgt tgccaccaag gtggcgccac agtataaact gccgcgaatg
     6121 agcgctccgt ccgtaacata tcctctcctt gaatctatca atgcgccgga ggatttacgg
     6181 cggctgccga ccaccaaact gcccgggttg gccgccgaac tgcgccaatt cctgatccaa
     6241 agcgtcagca cgcgcggcgg ccactttgcc gcgggcctcg gcacggttga actgacgatt
     6301 gccctgcatt atgtgttcaa cacgccctat gaccggctgg tctgggatgt cggccaccag
     6361 gcttacccgc acaaggtgct gaccggacga cgcgaccgcc tgcacaccat caagcagcac
     6421 cagggtctgg cgccttttcc gacccgctcc gaaagcgaat tcgacacgtt tggcgtcggc
     6481 cactcgagta cttcgatcag cgcagcgctc gggatggccg tcgcggcggc gcaacgcggc
     6541 gaagagcggc gtgccgtggc catcatcggc gatggcgccc tcactgccgg catggccttt
     6601 gagg
//
LOCUS       DASWZT010000359         6142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_17302, whole genome shotgun sequence.
ACCESSION   DASWZT010000359 DASWZT010000000
VERSION     DASWZT010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6142
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17302"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..2988
                     /gene="cphA"
                     /locus_tag="VGE92_10005"
     CDS             160..2988
                     /gene="cphA"
                     /locus_tag="VGE92_10005"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY1314201.1"
                     /translation="MRILDRSVYVGPSLYARFPVIRLELDLGALEAWPTGRLGPEFID
                     ALAAALPGLAEHGCSYKEPGGFYRRMREDDGTWLGHVLEHVAIELQNMAGEEVTFGKT
                     RSAGPPGVYTVIYEYAQRDEGIAAGDLGMRLLCSLLPASIRPADSVPEGWVWQEARDE
                     FIRFAQRRALGPSTAALVRAAEHHGIPWLRLNDQSLVQLGHGKFQQRIQATVTSRTSH
                     IAVELASDKEETNKILATLGLPVPKQELVQSEAQAVRAARRIGFPVVTKPYNGNHGRG
                     ISIRLTTDEEVAQGYVMAREHSRSVIVETFMEGDDHRLLVVNGELVAATRRTPGHVVG
                     DGQRNITQLIEIVNQDPRRGVGHEKVLTRLELDAQAHKMLERVAMTPDSIPTPGQVVY
                     LRSTANLSTGGTATDVTDIIHPDNREMAERAVRAIGLDVGGVDFLSKNITESYRTIGG
                     GICEVNAAPGFRMHVAPSEGKGRDVAAPVVGMLFPPGVASRVPIAAVTGTNGKTTTAR
                     MLAHLTKMSGYTPGLTTTDGVYIDGQRTVQGDMTGPVSARMVLADPQIDIAILETARG
                     GLLRAGMGVREINVGAVLNVQSDHLGLKGIDTLEQLAELKRIVVEVATDCAVLNADDP
                     LVLKMSGYTEAKNICYVTMNPQHTLVREHIRAGGRACALEAGVNGQMMTLYDRGSHIP
                     LLWTHLIPATLEGRATHNVQNAMFAAAMAFSLGIKLDAIRQGLRTFDSTFYQAPGRMN
                     IFNEHPFKVLFDYGHNAHAVGMLADLATRLDVTGRRIVVLAGPGDRRDEDLVAIAKAV
                     AGRFDHYICRRDDNLRNRAPDEVPRIQAQALRAAGVPESAISVIPDEQEAVDAALLMG
                     QPGDLLLIFADALVRSWKQITKFKPSGAAAPQPAAPEPPTAAARSVAEPKSNGSMAPL
                     SEAMAPNLNIEGIIRDERGIRMAPETED"
     gene            3003..4790
                     /locus_tag="VGE92_10010"
     CDS             3003..4790
                     /locus_tag="VGE92_10010"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEY1314202.1"
                     /translation="MPFEDSRRLTGGNLFFASTGAVLEALGELDEALLAAWRARIERA
                     AARLGWGHPQSAARRHAGGTSLAIAAPLDQLFVATEVNEWALCAALIERDPKLCRSLE
                     DALAAASLEALDSGATPGPDTIPVLEESAALARFVRLAAVEARPQLRALLEAAALRGL
                     PHVLDEAQLTLGAGAGGRSFPLSALPDPGELRWDQLRDIPTAIVTGSNGKTTTVRLLA
                     ACAREHGWHAAFNCTDGVFFDDELLASGDYSGPAGARMVLREPRAQAAILETARGGIL
                     RRGIAVSQARVAVVTNVSADHFGEYGIDDLAALADVKLAVSGIVSASGLLVLNADDAQ
                     LRARADGLTRRFGRCPRIAWFAQNADHPLLGESRLRGAPTCGVRAGTLCLSYDGTDHD
                     LGRVAALPLSVDGIATYNVANMAGAALGAAALGIAAATIATVFARFGAHFADNPGRMM
                     RFDRHGVRILIDYAHNPDGLRGLLTVAEHLRGTNGRLGLLLGHAGNRNDADIEELARV
                     AAAFQPDLVVVKENEAQLRGRAPGEIPRILLAELLRRGISESALTVRDSEAEAARCAL
                     DWARSGDVLALPLHGRGARAEIVALLEGG"
     gene            complement(4811..>6142)
                     /locus_tag="VGE92_10015"
     CDS             complement(4811..>6142)
                     /locus_tag="VGE92_10015"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HEY1314203.1"
                     /translation="ECTATVTELLPLDQPRYLMGVGTTRDLLEAVHRGVDMFDCILPT
                     SLARQGVAFTSLGRRDLRRGAYRGIEGPLDPACECYTCKTYSIAYLFHLQRVHESTGW
                     QFLSLHNVHFYARLMRTMREHIVADTWLPFYRAQRELLDARDSYGKPPAQVTRAARRK
                     LELRRGRFEVVVRQDVGFIRDMTSGEVMHAIDDPALEARSLYVEQSRLIERLQAPAAA
                     PLVVWDVGLGAAANAMAAILAVEAASCGTQRQLTVVSFEHDLDSLKLALDHVRWFKHL
                     RHAAPRVLLAQSRWIGRAGGIDWSLLCGDFAARKFDAPPPDIIFFDPFSLKTDASLWT
                     LSAFRELALVCAPKPMELFTYSCSTSVRAALLAAGFFVAKGRGTGPKAETTIALSPSA
                     ASAAHAHELLGNDWLARWNRSDAQAPCGAVADDSSWRAAVTRHPQFAASSA"
BASE COUNT         1118 a         2002 c         1990 g         1032 t
ORIGIN      
        1 aggtcggcga gggccagccg gtctgcatgc tcgggctcaa gttgcacatt ctggtcgccg
       61 gtgcgacatt caacttgcat acccgaacgg catcggccgg cgggttggtc caggccaaac
      121 aataatagat tgccgcagag cgcggcggag caacgcccca tgcgtattct cgatcgttcc
      181 gtctatgtcg gcccttcgct gtacgcgcgc ttccccgtca tccggctcga actcgatttg
      241 ggcgcgctcg aggcttggcc gaccgggcga ctgggaccgg aattcatcga tgcactggct
      301 gccgcactgc ccgggctcgc cgagcacggc tgctcctata aggagccggg cggtttctac
      361 cgtcgcatgc gcgaagacga cggtacgtgg ctcgggcacg tgctcgagca cgtggccatc
      421 gaactgcaga acatggcggg cgaagaagtg acgttcggca agacccgcag cgccggtcca
      481 cccggggttt acacggttat ctacgagtac gcgcagcgcg atgaaggcat cgcggccggc
      541 gacttgggca tgcggctgct ctgttcgcta ctgcccgcat ccattcgccc cgccgactcg
      601 gtgcccgagg gctgggtttg gcaggaggcg cgcgatgaat tcattcgctt cgcgcaacgc
      661 cgcgcgctcg gcccttcgac tgccgccctg gtgcgcgcgg cggaacacca cggcatcccg
      721 tggctgcgcc tcaatgatca atcgctggtg cagttaggcc acggcaagtt ccaacagcgc
      781 attcaagcaa ccgtgaccag ccgcacctcg cacatcgccg tggaactcgc cagcgacaaa
      841 gaagaaacca acaagatcct ggccacgctt ggcctgccgg tgccgaagca agaactggtg
      901 caaagcgaag cgcaagccgt gcgcgccgcg cggcgcatcg ggtttcccgt ggtgacgaag
      961 ccctacaacg gcaatcacgg ccgcggcatc tccatccgac tcaccaccga tgaggaagtg
     1021 gcgcagggtt acgtcatggc tcgcgagcac tcgcgctccg tcatcgtcga aacgttcatg
     1081 gaaggcgatg atcatcggct gctggtcgtg aacggcgaac tggtcgcggc gacgcgccgc
     1141 acgccggggc acgtcgttgg cgacggccaa cgcaatatta cgcagttgat cgagatcgtg
     1201 aatcaggacc cgcgccgcgg cgtcggtcac gagaaggtgc tcacgcgtct ggaactcgac
     1261 gcccaagctc acaaaatgct cgagcgcgtg gccatgaccc ccgactccat cccgacgccg
     1321 ggccaggtcg tgtatctgcg ctccaccgcc aacctctcca ccggcggcac cgcgaccgat
     1381 gtcaccgaca tcatccatcc tgataatcgc gaaatggccg agcgtgcggt gcgtgccatc
     1441 ggcctcgatg tcggcggcgt ggattttctc tccaaaaata tcaccgagag ctatcgcacg
     1501 atcggcggcg gtatctgcga ggtaaacgcg gcgccgggct ttcgcatgca cgtcgcgccg
     1561 agtgaaggca agggacgcga tgtcgcggcg cccgtcgtcg gcatgctgtt cccgcccggg
     1621 gttgcctcac gcgtgccgat cgcggcggtc accggcacca acggcaagac caccaccgcc
     1681 cgcatgctcg cgcatctaac gaagatgagc ggttatacgc cggggttgac caccacggat
     1741 ggcgtttaca tcgacggcca acgcaccgta cagggcgata tgacgggccc ggtctcggcg
     1801 cgcatggtgc tcgccgatcc gcagatcgat atcgccattc tcgagactgc ccgcggcggc
     1861 ctgctgcgtg ccggcatggg cgttcgcgaa atcaacgtcg gcgccgtgct caacgtgcag
     1921 tcggaccatt tgggcctgaa aggcatcgac acgctcgaac aactcgcgga gctcaagcgc
     1981 atcgtggtcg aggtcgcgac ggactgcgcg gtgttgaacg cggatgatcc tctggtgctc
     2041 aagatgtcgg ggtacacgga agccaagaac atctgctacg tgaccatgaa cccacagcac
     2101 acgctggtgc gcgagcacat tcgtgcgggc ggaagagcct gcgcgctcga ggccggcgtc
     2161 aacggtcaaa tgatgacttt gtacgaccgc ggcagccaca ttcccctgct ctggactcac
     2221 ttgattccgg caaccctcga gggccgcgcc actcacaacg tacaaaacgc catgtttgcc
     2281 gcggcgatgg cgttttcgct cggcattaaa ctggatgcaa ttcgtcaagg actgcgcact
     2341 ttcgattcga cgttctatca agcgcccggg cgcatgaaca tcttcaacga gcacccgttc
     2401 aaggtgctgt tcgattatgg gcacaacgcg cacgccgtcg gcatgctggc ggatctggcg
     2461 acgcgtctcg acgtcaccgg gcggcgcatc gtcgtgttgg cgggtcccgg tgatcggcgc
     2521 gacgaagacc tggtggcgat cgcgaaggcg gttgccggcc gcttcgatca ctacatttgc
     2581 cggcgcgacg acaatctgcg caatcgcgca ccggacgaag tgccgcgcat acaagcccag
     2641 gctttgcgcg ctgccggcgt acccgagagc gcaatttcgg tcatccccga cgagcaggag
     2701 gccgtcgacg cagccctact gatgggccag ccgggagact tgctgctgat tttcgccgac
     2761 gccttggtgc gctcgtggaa acaaatcacc aagttcaagc cgagcggcgc agccgcgccg
     2821 caacccgcgg cgccggagcc gcccacggcg gcggcgcgca gcgtcgcgga gcccaagagc
     2881 aacgggtcga tggcaccgct ttcggaggcg atggcgccga acctcaacat cgaagggatc
     2941 atccgcgacg agcgcggcat tcgcatggcg ccggagactg aagactgaac gttccgcacg
     3001 cgttgccgtt tgaggattcc cggcgcctca cgggcggcaa tctgttcttt gcatctacag
     3061 gtgcggttct cgaagccctg ggcgagctcg atgaggcgct gctcgcggcg tggcgcgcac
     3121 ggatcgagcg cgccgctgcg cgcctcggat ggggtcatcc gcagtcagcg gcgcggcgcc
     3181 acgccggcgg cacctccctg gcgatcgccg caccgctcga tcaattgttc gtggcgaccg
     3241 aagtcaacga atgggcgctc tgcgcggcgc tgatcgagcg ggatccgaag ctgtgccgca
     3301 gcctcgaaga tgcactcgcc gcggcatcgc tcgaagccct ggacagcggt gcaactccag
     3361 gcccggatac cattccggtg ctggaggaat ccgcggcgct ggcacgcttc gtacggctcg
     3421 ctgccgtgga agcgcggccg cagctgcgtg cactcctcga ggctgctgca ctgcgcggac
     3481 tgccgcatgt gctcgacgaa gcgcagctca cgctgggcgc gggtgccgga ggtcggagtt
     3541 ttcccttgag cgcgctgccc gatccaggcg agttgcgttg ggatcagctg cgcgacattc
     3601 caacggcgat cgtcacgggt tctaacggca agacgacgac ggtgcgcctg cttgccgcct
     3661 gcgcccggga gcacggttgg cacgcggcgt tcaattgcac cgatggggta tttttcgatg
     3721 atgagttgct cgccagcggc gattattccg ggcccgccgg cgcccgcatg gtgttgcgcg
     3781 agccgcgcgc gcaggcggcc attctggaga ccgcgcgcgg cggcatactg cggcgcggaa
     3841 ttgcggtatc gcaggcacgt gtcgcggtgg tcaccaatgt cagcgcggat catttcggcg
     3901 aatatggcat cgacgacctc gctgccctgg ccgatgtgaa actggcggtt tccggcatcg
     3961 tgtcggcgag cggcctattg gtactgaacg ccgatgatgc gcagttgcgc gcgcgcgcag
     4021 acggcctgac gcgccgcttc ggacgctgcc cgcgcatcgc ctggttcgcg cagaacgccg
     4081 accatccgct gcttggcgag agtcgcttgc gcggcgcacc cacctgcggc gttcgcgcgg
     4141 gaacgttgtg cctgagttac gacggcaccg atcacgatct cggacgggtg gcggcgctgc
     4201 cgttgtcggt cgacggcatc gcaacctaca acgtggccaa catggccggc gccgcgctcg
     4261 gcgccgctgc actgggaata gcggccgcga ccatcgcgac cgtgttcgca cgtttcggag
     4321 cccatttcgc ggacaatccg ggccgcatga tgcgtttcga ccgccatggg gtgcgcatcc
     4381 tgatcgacta cgcccacaat cccgacggcc tgcgcggatt attgacggtc gccgaacatc
     4441 tgcgcggcac caacggtcgt ctaggattgc tgttaggtca cgccggcaac cgcaacgatg
     4501 ccgatatcga ggaattggcg cgcgtcgccg ccgcgtttca acccgacctc gtggtcgtca
     4561 aagagaacga agcgcaactg cgcggacgcg cgcccggcga aatcccgcgg atactcttgg
     4621 ccgagttgct acgccgggga atctcagaat ccgcattgac ggtgcgcgac agcgaagccg
     4681 aggcagctcg ctgcgcgctc gactgggcgc gttccggcga cgtgcttgcg ctacccctgc
     4741 acggccgggg cgcgcgcgcc gagatcgtgg cattgttgga aggcggctag cactcgcggc
     4801 ggcatttgac ttaagcagaa ctcgccgcga actgcggatg ccgagtcacc gctgcccgcc
     4861 acgacgaatc atcggcaacg gcaccgcagg gcgcctgtgc atcgctgcgg ttccaacggg
     4921 cgagccaatc atttccgagc aattcatgcg catgcgccgc gctcgccgcg cttggcgata
     4981 gagcgatggt cgtctcagct ttagggccgg ttccgcgccc ctttgcaaca aagaatccgg
     5041 cggccagcag ggccgcgcgg acgctggtcg agcagctata ggtgaacaat tccatgggct
     5101 tcggcgcgca caccagagcc aactcgcgaa acgccgacag cgtccacaac gaggcatcgg
     5161 ttttcaacga gaacgggtcg aagaatatga tgtcgggcgg cggcgcatcg aattttcggg
     5221 cggcaaaatc gccgcacagg agcgaccaat cgatgccgcc cgcacgaccg atccagcggc
     5281 tttgcgccaa caatacccgc ggcgccgcgt ggcgcaggtg cttgaaccat ctgacatggt
     5341 cgagggcgag tttgagcgaa tccagatcgt gctcgaagct caccacagtc aattggcgct
     5401 gcgtgccgca gctcgccgct tcgaccgcga gaattgccgc catggcattg gcggcggcgc
     5461 ccaatcccac atcccaaaca accagcggcg cggccgcggg cgcttgcagt cgttcgatca
     5521 gccgcgactg ttcgacgtag agcgaacgtg cctccagcgc cggatcatcg atggcgtgca
     5581 tgacctcgcc ggaagtcatg tcacgaataa atccgacatc ctgccgcacg acgacctcga
     5641 agcggccacg ccgcagctcg agtttgcggc gtgcggcgcg ggtgacctgc gcgggaggct
     5701 taccgtagct gtcccgcgcg tcgagcagct cgcgctgggc tctatagaac ggcaaccaag
     5761 tatccgcgac aatgtgctcg cgcatggtgc gcatcagtcg cgcatagaaa tgcacgttgt
     5821 gcagactcaa gaattgccag ccggtgctct cgtgcacgcg ttgcagatga aaaagatagg
     5881 cgatcgagta ggtcttgcac gtataacact cgcaggcagg atcgagcgga ccctcgatgc
     5941 cgcgataggc gccacgccgc agatcgcgcc gcccgagact ggtgaatgcc acgccctgtc
     6001 gagcaagcga ggtcggcaga atgcaatcaa acatgtcgac gccgcgatgc acggcctcga
     6061 gaagatcgcg tgtcgtgccg actcccatca aatagcgcgg ctgatcgaga ggcagcagtt
     6121 ccgtgacggt ggccgtgcat tc
//
LOCUS       DASWZT010000360         5536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_2857, whole genome shotgun sequence.
ACCESSION   DASWZT010000360 DASWZT010000000
VERSION     DASWZT010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2857"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..578)
                     /locus_tag="VGE92_10020"
     CDS             complement(18..578)
                     /locus_tag="VGE92_10020"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY1314204.1"
                     /translation="MTSLALFPLNMVLFPAGALPLRIFETRYVDMVRRCMRETQAFGV
                     VLIREGQETGPAETFDVGTLARIVDFHQLSDGLLGLSCLGELRFRIHSRSCQADGLNL
                     AEVDWLVPEAAVPVPERHARLPRLLKTVLPQLGEVYTNMPMHLDDAAWVGHRLAEILP
                     IPLEDKQRCLELDDPIQRLDVLAPLA"
     gene            complement(575..1981)
                     /gene="mpl"
                     /locus_tag="VGE92_10025"
     CDS             complement(575..1981)
                     /gene="mpl"
                     /locus_tag="VGE92_10025"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008843230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEY1314205.1"
                     /translation="MAAGHMTQHVHILGIAGTFMGGVAAIAKAAGYRVTGADLNVYPP
                     MSTQLESLDIEFIQGYGAEQLNLNPDMVVVGNALSRGSPVIEAMLNRGMAYTSGPQWL
                     AEQVLHRKHVLAVAGTHGKTTTTAMLTWILEDAGLEPGFLIGGVPSNLDATARFGAGR
                     CFVIEADEYDTAFFDKRAKFVHYRPRTAILNNLEYDHADIYPDVAAIRRQFNQLLRTV
                     PADGKIIVNGEDLELATTLSLGCWTPRETFALTRNPDAAAGGDWSAAIAPDSAASRFT
                     VELQGRPLAEVNWQLLGEHNVRNALAAIAAARHVGVPPQRAVQALNAFRGVKRRMEVR
                     GVIDQVTVYDDFAHHPTAIETTLHGLRSRVGGARIIAVFEARSNTMKLGVHRDHLAPA
                     FKLADRVWFLNSADLGWDLQGAVAAMGDRARFAPSVDAIVQELAADSRAGDHILIMSN
                     GGFGGLHDKLLAALQARR"
     gene            complement(1963..2478)
                     /locus_tag="VGE92_10030"
     CDS             complement(1963..2478)
                     /locus_tag="VGE92_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314206.1"
                     /translation="MLRLAKAFWDIALWRRTPAQLPASSFLLTLVCIAAALLEVVSAF
                     LPPVSSEHIVTRIVLSVAVPLGFAWAVLAVAHRRQRFLQTATALLGVGVLVEIALYPI
                     GSLIHIIGSEQLAAVPLGVLMLTGFIWYLLACANIWRAALDSGLSLGAVVSVGYILLS
                     IVLEQQWLPDT"
     gene            2529..3599
                     /locus_tag="VGE92_10035"
     CDS             2529..3599
                     /locus_tag="VGE92_10035"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HEY1314207.1"
                     /translation="MPDAIWIDRDDQLPDLARQLESQPWIGVDTEFLRERTFFPKLCL
                     LQLCIRGQIWCVDTLALRGLDPLMPALTAAGARKVIHAARQDLEAVYLSAKRVITPVF
                     DTQIAAGCIGLKPQVGYAELVKTLLDVTVPKGQTRTDWSKRPLTKEQLEYAADDVLYL
                     GDVEQRLSTRLRELGREQWVAEDCLALQDKQLYEPDPSQAWVRLRGLAQLAPASRARA
                     KATAVWREKLARSRDLPRAWILSDAAILSIAEGAPPPLNDGLAANLRDSLEEAANDAG
                     EEEPSQDARPTAEQKALIERLGKIVDARAAELKVSAEILAPRGELKALALGTRGGALR
                     EAHALTGWRREEIGTRLLAAIG"
     gene            complement(3589..4260)
                     /locus_tag="VGE92_10040"
     CDS             complement(3589..4260)
                     /locus_tag="VGE92_10040"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001380.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEY1314208.1"
                     /translation="MRIVLLGAPGSGKGTQSQLLVKSFGIPQISTGDLLREAVAKGSE
                     LGLRAKAAMDSGKLVDDATVLGMIRERIAKPDAVNGFILDGFPRNIAQAEALQKMLGS
                     IGTPLDAVVLMNLDLGILFKRLTGRRICQDCGQVFNVYTAPADNGAPSACGASHRLIQ
                     RPDDREEVIGKRLEVFEAQTKPLIKYYQSAGLLRSVDADADVSTVFASIDRALRGPPA
                     DGVSR"
     gene            4362..>5536
                     /locus_tag="VGE92_10045"
     CDS             4362..>5536
                     /locus_tag="VGE92_10045"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEY1314209.1"
                     /translation="MHNAFYAQSGGVTAVINASACGLIETARRHRDKIGKVYAGRHGI
                     IGALTEDMIDTSKESRAAIAALRYTPGGAFGSARYKLAGIDKNRAQYERLIDIFRAHD
                     IGYFFYNGGNDSMDTAQKVSEIAVQMQYPIACIGIPKTVDNDLPVTDCCPGFGSAAKY
                     IAVSTREAALDVASMARTSTKVFVLEVMGRHAGWMAAAGGLAGEKAGDAPHIILFPEI
                     PFDRGRFLSKVHDTVEKYGYCVVVVSEGVRNPDGKFLAEAGTKDAFGHAQLGGVGPVV
                     AQMTQEAFGYKFHWAVADYLQRSARHIASKVDVDQAYAVGKAAVELAVRGKSAVMPVI
                     IRKSSKPYRWSIGEAPLSAVANQEKKLPRNFISADGFGITTACRAYLSPLIGGEDYPP
                     "
BASE COUNT         1082 a         1769 c         1722 g          963 t
ORIGIN      
        1 cgcccggcgt gggtgttcta cgccaagggc gccagcacat cgagccgctg aatcggatcg
       61 tcgagttcca ggcaccgttg tttgtcctcg agcggaatcg gcaggatctc ggcgaggcgg
      121 tgtcccaccc aggccgcatc gtccaagtgc atcggcatat tggtgtagac ctcgcctagt
      181 tgtggcaaca ccgttttcaa gagccgcggc aatcgtgcat gtcgctccgg caccggcacg
      241 gcagcctcgg gcacgagcca gtcgacttct gccaaattga ggccgtccgc ttggcagctg
      301 cggctgtgaa tgcgaaaacg cagctcgccg aggcacgaaa gccccagcag tccgtcggac
      361 agctgatgga aatcgacgat tctcgccaaa gtgccgacat cgaaagtctc ggcgggtccc
      421 gtttcctggc cctcgcgaat caataccacg ccgaaggcct gggtctcgcg catacagcgg
      481 cgcaccatgt cgacgtagcg ggtttcaaaa attcgcagcg gcaacgcccc ggcgggaaac
      541 aacaccatat tgagcggaaa cagggcgagc gaggtcaccg ccgtgcctgt aaggcggcga
      601 gcaacttgtc gtgcaaaccg ccgaaaccgc cgttgctcat gatcagaatg tggtcgcccg
      661 cgcggctgtc ggcagccaac tcctgcacga ttgcatcgac gctcggtgca aagcgcgcgc
      721 gatcccccat cgcggcgacc gcgccttgca gatcccagcc caagtccgcc gagttcaaga
      781 accagactcg atctgccagc ttgaacgcgg gcgccaggtg atcgcgatgc acgcccaatt
      841 tcatggtgtt cgagcgcgct tcgaacacgg cgatgatgcg cgcgccgccg acgcgcgagc
      901 gcagtccgtg caacgtcgtc tcaatggccg tcggatggtg cgcgaaatcg tcgtacaccg
      961 tcacttggtc gatcacgccg cgcacttcca tgcggcgctt cacgccgcga aaggcgttga
     1021 gcgcctgcac tgcgcgctgc ggcggcacgc cgacatggcg ggcagcggcg atcgccgcca
     1081 gcgcattcct cacgttgtgc tcgccaagca gctgccagtt cacttcggcc agcggccgcc
     1141 cttgcaactc aaccgtaaag cggcttgcgg cggaatccgg cgcgatcgca gcgctccagt
     1201 cgccgcccgc ggctgcgtcg gggttgcggg tcagtgcaaa agtctcgcgc ggcgtccaac
     1261 aaccgaggct caaggtggtt gcgagctcga gatcctcgcc gttgacgatg atcttgccgt
     1321 cggccggcac ggtgcgcagc aactgattga attgacggcg gatcgcggcc acgtccggat
     1381 aaatatccgc gtgatcgtat tccaaattat tgaggatggc ggtgcgcggt cggtagtgga
     1441 cgaatttcgc acgcttgtcg aaaaacgccg tgtcgtattc gtcggcctcg atgacgaagc
     1501 agcgccctgc accgaagcgt gcggtggcat cgaggttgct cggcacaccg ccgatgagaa
     1561 atcccggctc gagaccggca tcctcgagga tccacgtcag catcgcggtg gtcgtggtct
     1621 tgccatgcgt gcctgccacg gccagcacat gtttgcggtg caggacttgc tccgccagcc
     1681 actgcggccc ggaggtatac gccattccgc gattcagcat ggcctcgatc accggactgc
     1741 cgcggctcaa cgcatttccc acgaccacca tgtcgggatt gaggttcaac tgttcggcac
     1801 cgtagccttg aatgaactcg atgtcgagcg actcgagctg agtgctcatc ggcggataaa
     1861 cgttgagatc cgcaccggtc acgcgatatc ccgccgcctt ggcgatggcc gcgacgccgc
     1921 ccatgaaagt cccggcgatc cccaatatgt gcacgtgctg cgtcatgtgt ccggcagcca
     1981 ttgctgttcc aggacgatcg acaacaaaat gtagcccacg ctgaccacgg cacccaagct
     2041 caatcccgaa tcgagcgcgg cgcgccaaat gttggcgcag gcgagcaagt accaaatgaa
     2101 gcccgtcagc atcagcaccc ccagaggcac ggccgcgagc tgttccgatc cgataatatg
     2161 aatcagcgat ccaatcggat acaaggcgat ctccaccaac actccgactc ccaatagcgc
     2221 ggttgccgtt tgcaggaagc gctgccggcg gtgcgccact gccagaactg cccaggcaaa
     2281 accgagcggc acagccacgc tcaacacgat acgggtgacg atatgctcac tggaaacggg
     2341 tggcagaaac gcgctgacca cctccagcag cgccgccgcg atgcacacca gcgtcagcag
     2401 gaacgaggac gccggcaatt gcgccggtgt tcgcctccaa agagcgatgt cccagaatgc
     2461 ttttgcgagt cgcagcatcg tccaattcag tcataatcgt ttccaaattg tacacgcgga
     2521 tcaccggcat gcctgacgcg atttggatcg atcgagatga ccaattaccg gacctggcgc
     2581 ggcagctgga gtcgcagcca tggatcgggg tcgatacgga attcctgcgc gagcgcacat
     2641 tctttcccaa gctctgcctg ctgcagctgt gcatccgggg ccaaatctgg tgcgtcgaca
     2701 ccttggcgct gcgcgggctc gatccattga tgccggcgct gactgccgcg ggagcgcgca
     2761 aggtgatcca tgccgcacgc caggatttgg aggcggtgta tctgtcggcc aagcgcgtca
     2821 ttacgccggt attcgatacc cagatcgccg ccggctgcat tggcctcaag cctcaagtgg
     2881 gctacgcgga actcgtcaag acattgctgg atgtgaccgt gcccaagggc cagacgcgca
     2941 ccgactggtc caagcggccg ctcaccaagg aacagctgga atacgcagcc gatgatgtct
     3001 tgtacctagg cgacgtcgag cagcggttat cgacgcgtct gcgcgagctc gggcgcgagc
     3061 agtgggtggc ggaggattgc ttggcactcc aagacaagca gctatacgag cccgatccct
     3121 cgcaagcatg ggtgaggctg cgcggcctcg cgcaactcgc gccggcgtcg cgggcccgcg
     3181 ccaaggccac cgcagtgtgg cgcgagaagc tggcgcgcag ccgcgacctg ccgcgcgcct
     3241 ggatcttgtc ggatgcggcc atactctcga ttgccgaggg cgcgccgccg ccgctcaacg
     3301 acggcctcgc cgcaaacctg cgcgattcgc tcgaggaggc tgccaacgac gccggggagg
     3361 aggaaccgag ccaggatgca cggccgaccg ccgagcaaaa agccttgatc gagcgcctcg
     3421 ggaaaatcgt cgatgcgcgc gcggccgagc tgaaggtgag cgccgagatt ctcgcaccgc
     3481 gcggcgaact caaggcgctt gccttgggca cgcgcggcgg cgcgctgcgc gaggcgcatg
     3541 cattgacggg ttggcgccgc gaagaaatcg ggacccggct gctcgcggct atcggctgac
     3601 gccgtccgcg ggcgggccgc gcagcgcacg atcgatgctc gcgaacaccg tcgacacatc
     3661 ggcgtcggca tcgacgctgc gcaacaggcc ggcactctga taatacttga tgaggggctt
     3721 ggtctgcgct tcgaacacct cgagccgctt gccgatgact tcttcgcggt catcgggtcg
     3781 ttgaatgaga cgatgcgagg cgccgcatgc ggacggtgcg ccgttgtcag ccggcgcggt
     3841 atagacattg aagacctgtc cgcagtcctg gcagattctg cggccggtca agcgcttgaa
     3901 gagaatgccc aaatccagat tcatgagcac caccgcatcg agcggcgtgc cgatcgagcc
     3961 gagcattttc tgcagagcct cggcttgggc gatgttgcgc ggaaagccat ccaaaatgaa
     4021 accattgacc gcgtccggtt tcgcgatgcg ttcgcggatc atgccgagta cggtggcatc
     4081 atcgaccagc ttgcccgagt ccatggccgc cttggcgcgc agcccgagtt cgctaccctt
     4141 cgcaacggct tcgcgcaaca ggtcgccggt cgagatctgc gggatcccga aggatttgac
     4201 cagcagctgc gactgcgtgc ctttgcccga gccgggcgcc cccaatagaa ctatgcgcat
     4261 aattatttga gtagtgaaag cacactaccg taccctttgg gcggcgcggt cgtcaaaccc
     4321 cagccgatcg gctattctgc gcgccttgcg cgggggtatt catgcataac gcgttctacg
     4381 cacaatccgg cggagtgacc gccgtcatca atgcctcggc ctgcggcctg atcgagacgg
     4441 cgcgccggca tcgcgacaag atcggcaagg tgtacgcggg ccggcacggc atcatcggtg
     4501 cattgaccga ggatatgatc gacaccagca aggagagccg cgccgcgatc gccgctctgc
     4561 gctacacacc gggcggcgcc ttcggctcgg cacgctacaa gcttgccggg atcgacaaga
     4621 atcgtgccca gtacgaacgc ctcatagaca tattccgtgc gcatgatatc ggctatttct
     4681 tctataacgg cggcaacgat tccatggaca ccgcgcaaaa ggtgtcggaa atcgcggtgc
     4741 aaatgcagta tcccatcgcc tgcatcggaa tccccaaaac cgtggacaac gacctgccgg
     4801 tcaccgattg ttgcccggga ttcggctccg ccgccaagta cattgccgtg tcgacgcgcg
     4861 aggcggcgct cgatgtcgcg tcgatggcgc gcacctcgac caaggtattc gtgctggaag
     4921 tcatggggcg gcacgccggt tggatggccg cggccggtgg ccttgccggc gagaaagccg
     4981 gcgatgcgcc gcacatcatt ctgtttccgg aaatcccctt cgaccggggc cgatttctct
     5041 cgaaggtgca tgacacggtc gagaaatatg gctactgcgt cgtcgtagtc tccgaaggcg
     5101 tgcgcaatcc cgacggtaaa ttcctcgccg aggcgggcac caaggatgcc tttggccatg
     5161 cacaactggg aggcgtcggg cctgtagtcg cacagatgac ccaggaagcc tttggctaca
     5221 agtttcattg ggcggtcgcc gattacctgc agcgttccgc gcgccacatt gcatccaagg
     5281 tggatgtcga ccaggcctat gccgtcggca aggcggccgt ggaactggcc gtacgcggta
     5341 aaagcgccgt gatgccggta attattcgca aatcctccaa gccttaccgc tggagcatcg
     5401 gtgaagcgcc tttgagcgcg gtcgcaaacc aggagaaaaa gctaccgcga aacttcatca
     5461 gcgccgatgg gttcggcatc accacggcct gccgcgcgta cttgtcgccg ctgatcggcg
     5521 gcgaagacta cccgcc
//
LOCUS       DASWZT010000361         4991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_41733, whole genome shotgun sequence.
ACCESSION   DASWZT010000361 DASWZT010000000
VERSION     DASWZT010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41733"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VGE92_10050"
     CDS             complement(<1..206)
                     /locus_tag="VGE92_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314210.1"
                     /translation="MSKVLVVFRRELRSYFATPLAYVFIVIFLVLAAVFTFQVGGFFE
                     RGQADLQSFFRWHPWLYLVLIPAIS"
     gene            complement(203..1168)
                     /locus_tag="VGE92_10055"
     CDS             complement(203..1168)
                     /locus_tag="VGE92_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1314211.1"
                     /translation="MITTKNLSKRYGAKLAVDDLTFSVAPGEVLGFLGANGAGKSTTM
                     RMIAGFIAPSAGEVSVCGHDIERNPVAAKSCMGYLPEGAPSYGEMTVSEFLDFVADIR
                     GLSGELRRTRRAVVVDRLGLASVIEQVVETLSKGFRRRVGLAQALIHDPQVLILDEPT
                     DGLDPNQKHEVRRLINELSKDKLVIVSTHILEEVHEVCTRAIIIADGRIVADETPTAL
                     EARSRYHHAVSLRFEKREQLEAARREIALLAEVAEVESDARELRLTAVPKAGANPLAA
                     VSAVIDKNAWDVPELHLEPGRLDEVFRSLTQQPRIDVSDADGDPA"
     gene            complement(1245..1994)
                     /locus_tag="VGE92_10060"
     CDS             complement(1245..1994)
                     /locus_tag="VGE92_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314212.1"
                     /translation="MATPADQRLLDLLEKWLKSLELHLKYSTLDDDSYWKTQTWVKHQ
                     RPSRWIIDLAMQKTRALRAQVEERMAAGDEKYSDSLELMSFLANLVGVEHIERFIPLA
                     GAENEHSLAPEPTPQPSAEEPPGHGVPAADASTGTREMPRFSAVKRGPPPAGTAQVAR
                     SERKAPAPAKSAAKKPAAPLKPQPRAVAALSDAAREQVMADAARLVQWGRKWYELPEL
                     IARMAGRPSLPDVRRILKENKAAIDRLAGNA"
     gene            complement(2054..2962)
                     /locus_tag="VGE92_10065"
     CDS             complement(2054..2962)
                     /locus_tag="VGE92_10065"
                     /inference="COORDINATES: protein motif:HMM:NF039471.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6492 family protein"
                     /protein_id="HEY1314213.1"
                     /translation="MPSICIVTPTYVGDAAQFTLLRRSINAFAPAFPHLAIVNSEDFA
                     EFRERFAGESNLEIIKSADVLPRAIEYRRRRSGPKWLTAMRRVPGRLIKGWHAQQLMK
                     LFVLRECSYEAVAFCDSDVLICRPIAADYFYLGDRLKLYRRRARDAECMDFDIAAHEI
                     LGNPLERITELYDYIYSPATFRKSTAVRLFAEFHSRHRSWVRRFLAQKRASEYHLLGY
                     AATVLEGGAGYQIVDCNPDDVHHSVRYETDRAELATVLQQIRAQHKDFALLQSRLNLN
                     VGQIAAAFDELRAASATRPAEPTSTR"
     gene            3081..4058
                     /locus_tag="VGE92_10070"
     CDS             3081..4058
                     /locus_tag="VGE92_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314214.1"
                     /translation="MAFENGVGNFRDVELVSGALRALGCAVTVTQVSVQARRRRRSAV
                     LRGMVAARAWGEKSRLIRARKKFDLNVMMEHVWPEHLPSAQRNVVIPNPEWFDHADQR
                     LLKLVDRVWAKTGHSYDAFTRLGCRVSMTGFDSEDRCDTAVPRDAGFFHLAGKSRMKG
                     TTQLVELWAKHPEWPTLDIVHSRKAAFKSIDAANIRYQTRYLADAELRALQNRHVFHL
                     CLSQTEGWGHYIGEAQSVGALLLTTDARPMNELVTVERGLLVESIESGKQHLAMTYSF
                     APADLERAVAVALGMSEHERTRLGSAARDWFLENKRGFGQRLNSALAEL"
     gene            4060..>4991
                     /locus_tag="VGE92_10075"
     CDS             4060..>4991
                     /locus_tag="VGE92_10075"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY1314215.1"
                     /translation="MPGTLVVRLCNWVGEVVLSVPSLRRLESAGYALHLVGKPWAPRL
                     LAGTGWPVTVRASGLLAAARQMRALKAQLAPHGAAPAPQALLMTKSLSSALETRLGGV
                     PPAGYAYDGRSWLLSRAFPLPKFEHAAFAYWQLVSDYLGVAAPYPTEVGLHPSETQTR
                     AAHALLATHALQPGGYCMLCPFSGADDRQGRKVWPGFRDLAQEFRTRGIALVVCPGVG
                     EEAMSAALPGVITLAGLDLGVYAALLASARTVIANDTGPGHLAAAVGARLISIFGPSS
                     SALWAPLGANVVLMHDAIWPSVERVVQASLETCP"
BASE COUNT          882 a         1538 c         1610 g          961 t
ORIGIN      
        1 gaaatggcgg gaatcaacac caagtagagc catggatgcc agcgaaagaa ggactgcaga
       61 tcggcctggc cgcgctcgaa gaagccgccc acttggaagg tgaacacggc tgccagcacc
      121 aggaagatca cgatgaatac gtaggccaac ggagtcgcga agtagctgcg cagttcgcgg
      181 cgaaacacca ccagaacctt gctcatgcgg ggtctccatc ggcatcggat acgtcgatgc
      241 gcggctgctg cgtcagcgaa cgaaacactt catcgagacg ccccggctcg aggtgcagct
      301 cgggcacatc ccaggcgttt ttgtcgatga ccgcgctcac cgccgccaag ggattcgcac
      361 ccgccttcgg caccgccgtg aggcgcaatt cccgggcatc ggactccacc tcggcaactt
      421 ccgccagcag cgcgatctcg cgccgcgcgg cctcgagctg ttcgcgcttt tcgaatcgca
      481 ggctgacggc gtggtgatat cgcgagcggg cttcgagcgc ggtcggcgtc tcatcggcga
      541 cgatgcggcc gtcggcgatg atgatggcgc gcgtgcacac ctcgtggacc tcctccaaga
      601 tatgagtgga aacgatcacc aacttgtcct tgctgagctc gttgatcaga cgccgtacct
      661 catgcttttg attcgggtcg agcccgtcgg tcggttcgtc caggatcaaa acctgcggat
      721 cgtggatcaa tgcctgagcg aggcccaccc ggcggcgaaa acccttggac agcgtttcga
      781 caacttgctc gatcacgctc gcgaggccca gccgatcgac gactaccgcg cggcgcgtgc
      841 gcctaagttc accgctcagt ccgcggatat cggcgacgaa atccagaaat tcactgacgg
      901 tcatttcgcc gtaactcggc gcgccctccg ggaggtagcc catgcaggac ttggccgcga
      961 ccgggttgcg ttcgatgtcg tggccacaga ccgaaacctc gccggcggac ggtgcaatga
     1021 atccggcgat catgcgcatg gtggtcgact tgcccgcgcc gttggcgccc aggaacccca
     1081 aaacctctcc cggcgcgacg ctgaacgtca agtcatcgac ggccagtttg gcgccgtaac
     1141 gtttgcttaa gtttttcgtc gttatcatgg gcgcctttga gcatgcttaa acgcaggagg
     1201 ttcaaaaaat tttctacgcg ataccgagag cattctgcga attgtcaggc gttccctgcc
     1261 agccgatcga tcgccgcttt attttccttg aggatgcgcc gcacgtcagg taacgatggg
     1321 cgccctgcca tgcgcgcgat gagctccggc aattcgtacc acttgcgtcc ccattgcacc
     1381 aagcgcgccg cgtcggccat cacttgttcg cgtgcggcat ccgatagagc ggcgaccgcc
     1441 ctcggctgcg gcttcaacgg cgccgccggc ttttttgccg cggactttgc gggtgcgggc
     1501 gctttacgtt ccgagcgcgc aacctgcgcc gtaccggcgg gcggcggtcc gcgtttaacg
     1561 gcggagaatc tcggcatctc gcgcgtgccg gtggaggcat ccgcggccgg cacgccatgc
     1621 ccgggcggct cttctgcgct tggctgcggc gtcggttccg gcgccaatga gtgctcgttc
     1681 tcggcgccgg ccagcgggat gaatcgctcg atgtgttcga ctcccaccag gttcgcgagg
     1741 aagctcatca actccaatga atccgaatac ttctcatctc cggccgccat gcgttcttca
     1801 acttgggcgc gcagtgcgcg ggttttttgc atcgccagat cgatgatcca gcggctcggc
     1861 cgttgatgtt tcacccaggt ctgggtcttc caataactgt cgtcatcgag ggtggagtac
     1921 ttgaggtgaa gctcaaggct tttgagccat ttttccaaga ggtccagcag tcgttgatcg
     1981 gcgggtgttg ccatgtcttt gcagctccag ggacagcacc ctaaacgaac cgccgcccga
     2041 tggccagcgc taactagcga gtggaggtgg gttcggccgg ccgcgtcgcg cttgccgccc
     2101 gcagttcgtc gaaggccgcg gctatttggc cgacattcag attcagtcgt gactgcagca
     2161 gcgcgaagtc tttgtgttgt gcgcgaattt gctgcagcac cgtcgccagt tccgcacggt
     2221 ctgtctcgta gcgcaccgaa tgatggacat cgtcgggatt gcagtcgacg atctggtagc
     2281 cggcgccgcc ctcgagcacg gtcgctgcat agcccaacag gtggtattcg gaggcgcgct
     2341 tctgcgcgag gaagcgccgc acccacgagc ggtggcggct atggaactcc gcaaataggc
     2401 gcaccgcggt cgatttgcga aacgtcgccg gcgagtagat gtaatcatag agttcggtga
     2461 tccgctcgag cggattgccc aatatttcat gggcagcgat gtcgaagtcc atgcattcgg
     2521 cgtcgcgggc ccgccggcgg taaagcttca agcggtcacc caggtagaag taatccgcag
     2581 cgatggggcg gcagatgagc acgtccgagt cgcagaacgc gacggcctcg tagctgcact
     2641 cgcggagcac gaacagcttc atcagctgct gggcgtgcca acccttgatc aatcgaccgg
     2701 ggacgcggcg catcgcggtg agccattttg gaccggatct tctgcgccgg tactcgatcg
     2761 ctcgcggcag cacgtcggcg ctcttgatga tctcaaggtt gctctcgccg gcgaaacgtt
     2821 cgcggaattc ggcgaaatct tcgctgttga cgatggcgag gtgcgggaat gccggcgcga
     2881 atgcattgat cgagcgtcgc agcagcgtga actgcgccgc gtcgccgacg tacgtcggcg
     2941 tgacgataca aatcgatggc atcggcaagc cccaaaagta taatataaat gagccgaatt
     3001 ttagtatagc gtaaggccaa gtccgcgctc gactccttga gtgccgcaaa gcgactatta
     3061 gagtgttaaa catcaacgtc gtggcatttg aaaacggagt cggaaatttc cgcgatgtcg
     3121 agcttgtgag cggtgcgctg cgcgcgctcg gttgcgccgt caccgtcacc caagtgagtg
     3181 tccaggcgcg ccgccggcgc cgttctgcgg tccttcgcgg catggttgcc gcgcgggcgt
     3241 ggggcgagaa gtcgcggctc attcgggcgc gcaagaagtt cgacctcaac gtcatgatgg
     3301 agcacgtttg gccggagcat ctgccctcgg cgcagcgcaa cgtggtgata ccgaatcctg
     3361 aatggttcga ccacgcggac cagcgactgc tcaaactcgt cgatcgcgtc tgggcgaaga
     3421 ccggccactc ctacgacgcg ttcacgcggc tcggctgccg cgtgagcatg acgggattcg
     3481 acagtgagga ccgttgcgac acggcggtgc cgcgcgacgc gggttttttt catctggcgg
     3541 gcaagagccg catgaagggt acgacgcagc tcgtcgagct ttgggcgaag cacccggaat
     3601 ggccgaccct cgacatcgtg cacagccgca aggcggcgtt caagagcatc gatgcggcca
     3661 atatccgcta tcaaacccgc tacctggcgg atgcggagct gcgcgcgctg cagaatcgtc
     3721 acgtatttca tctgtgtctg tcgcagaccg aaggctgggg gcactacatc ggcgaggcgc
     3781 aaagcgtcgg cgcattgttg ctcaccaccg atgcgcggcc gatgaacgag ttggtgaccg
     3841 tcgagcgcgg tctgctggtc gagtcgatcg agagcggcaa gcagcatctc gcaatgacct
     3901 actcgttcgc gccagcggat ctggagcgtg cggtcgcggt ggcgctcggc atgagcgagc
     3961 acgaacgcac gcggctcggt agcgccgcgc gggactggtt cttggaaaac aaacgcggtt
     4021 ttgggcagcg gttgaattcc gcgctggcag agttgtaagt tgccaggcac tctcgtggtt
     4081 cgtctgtgca actgggtggg cgaggttgta ttgtccgtgc cgtcgctgcg acgactcgag
     4141 tctgcgggtt acgccctgca tctggtcggc aagccgtggg cgccgcgcct gctcgccggc
     4201 accggctggc cggtaaccgt gcgcgcctcg ggcctgctcg ccgctgcgcg acaaatgcgc
     4261 gcgctcaagg cgcaactggc gccgcacggc gctgcgccgg caccgcaggc gttgctgatg
     4321 acgaaatctc tgtcttccgc gctcgaaacg cggctcggcg gagtgccccc cgcgggctac
     4381 gcctatgatg gcagaagctg gttgctgagc cgtgcgttcc cgctcccaaa gttcgaacac
     4441 gccgcgttcg cctattggca gctggtcagc gattatctgg gcgtggccgc gccgtacccg
     4501 acggaagtcg gcctgcaccc gagcgagact caaacgcgcg ccgcgcatgc actgctcgcc
     4561 acgcatgctt tgcagcccgg cggctattgc atgttgtgcc cgttctcggg tgccgacgat
     4621 cggcaaggac gcaaggtttg gcccggattt cgcgacctcg cgcaggaatt tcggacgcgg
     4681 ggaattgcgc tcgtggtttg cccgggcgtg ggcgaggaag ccatgagcgc ggcgcttccg
     4741 ggcgtgataa cactcgcggg tctcgacctt ggcgtctatg cggcgctgct cgcctcggcg
     4801 cgcacagtga tcgccaacga taccggcccg gggcacctcg ccgcggccgt cggtgcccgg
     4861 ctgatcagca tttttggtcc aagctcgagt gcgctgtggg cgccgctcgg tgcaaatgtg
     4921 gtactcatgc acgatgcgat ctggccgtcg gtcgaacgcg tcgtccaagc gagtctcgaa
     4981 acctgcccat g
//
LOCUS       DASWZT010000362        23621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_94927, whole genome shotgun sequence.
ACCESSION   DASWZT010000362 DASWZT010000000
VERSION     DASWZT010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 23621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23621
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94927"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VGE92_10080"
     CDS             <1..369
                     /locus_tag="VGE92_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314216.1"
                     /translation="PASTGTASTTAGANTGIAAYADRGIIAALKSLVQDLGNSQLISS
                     TDSSSSLSANTLSNLNSAFEKLIGDLGGSTSTSGGTSSTAGSQSTAALQSFLTGFLQD
                     LQNNGTQSLSALGSSVNTTA"
     gene            458..1813
                     /locus_tag="VGE92_10085"
     CDS             458..1813
                     /locus_tag="VGE92_10085"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1314217.1"
                     /translation="MATVPDVGGGADATPQRVNNRSLKIRQTITVLALFGGYAALYFC
                     RADLAVATPLLIEELGKHGVSHADAILRMGEITSVGVLAYAVGKLFLTGLGDFWGGRI
                     NFLIGLLGATLFTLLFASRLSLPLFSIAWIGNRLTQSIAWAGLVKVSSKWFDFSSYGM
                     IIGILSISYLVGDALARDWMGILIEHGFGWRALFYFAAAVGALFLAVNYFFLRESRID
                     AGFTEAKPNPLNLFAGSEAPPQSLGQLLLPLLRSHAFLLVCLLSLGCTIIRETFNAWT
                     PVYLRDYLGYSMGDAARMSGIFPGVGAVSVLVTGWLSDRLGVNGRSLIMFVGLAATAA
                     ALLLLMTMHSSLTGSLLPLIAIGIIAFCLLGPYSYLGGAFALDFGGKQAGAVSSGIID
                     GVGYLGAVAAGWAVARVAVSFGWQGVFVALALVSALSALGAGCLYLLGAKAAAHHTHL
                     P"
     gene            1810..2958
                     /locus_tag="VGE92_10090"
     CDS             1810..2958
                     /locus_tag="VGE92_10090"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY1314218.1"
                     /translation="MTVADVKRVSFSPDRLHVEWADGSLSEFASLWLRDNLREDRDPH
                     SGQRLIDIADLPQSPKIRAATAHNGCVSLDWADGERTATFELDWLLAHSSSHGGRRRG
                     RAHRLWLEAAGLAATRDFAWAVCNELAGHRAARAHWLTRLLQDGLGFLSDVPRNDAGI
                     LEAARLAGRVAETNYGLVFEVRSVAQPENLAYSDLGLGLHTDNPYREPVPGFQMLHVL
                     IASPDGGESLFGDGFAIAEHLRSTDREAFAVLTQTAVPFLYRSKDAELYAERPLIQLS
                     CDGEVTAVHYNSRSIAPLQRDARECERFYRAYRRFASLLRDPRFQLRFMLRDGDLAVF
                     DNQRILHGRTAFSSSRFARHLRGCYLTRDSVYSQAALLRREFPVDEPS"
     gene            2955..3497
                     /locus_tag="VGE92_10095"
     CDS             2955..3497
                     /locus_tag="VGE92_10095"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY1314219.1"
                     /translation="MSLADELFAIFHDRGAAEYFGERVSVTEHALQAAYFAQIASAPP
                     ALVIAALLHDIGHLVEQVPSDIADWKTDARHEVVGGEWLAKRFGPEVSEPVRLHVPAK
                     RYLLATDDAYITQLSPASLVTLKLQGGPMAAHEIAQFEAERYYADAVRVRRCDDQGKV
                     AGMKTPDFCDYRALIDALGR"
     gene            3518..4075
                     /locus_tag="VGE92_10100"
     CDS             3518..4075
                     /locus_tag="VGE92_10100"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEY1314220.1"
                     /translation="MKPKYFATAQSLRAWLHANAAASAELIVGFYKRDSGTASVTWPQ
                     AVDEALCVGWVDGVRHRIDERRYKIRFTPRKPSSIWSAINIERVRVLGAEGRMQPAGL
                     AAFGRRIEKNSRIYSYEQPGLAALTAQEQATFKRNKAAWAFFDAQPLGYKKRMLWRIV
                     SAKQPPTRERRFAALLEASAQGTRL"
     gene            4431..4856
                     /locus_tag="VGE92_10105"
     CDS             4431..4856
                     /locus_tag="VGE92_10105"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY1314221.1"
                     /translation="MTGVVFVDSNILIYAHDADAGAKRSRAADKLQELWESGGGRLSV
                     QVLQEFFVNVTRKLSTPVARSTAREVVSNYGTWIREPTTAATVIRAIDIAEMAQIQFW
                     DALIVASAEQADATRIYSEDLNNGQTIAGVQIVNPLLKT"
     gene            complement(5080..5850)
                     /locus_tag="VGE92_10110"
     CDS             complement(5080..5850)
                     /locus_tag="VGE92_10110"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY1314222.1"
                     /translation="MARLSGKVALVTGIGAGIGRECALMFAREGAVVMGCDIDASRAR
                     STVDAAKLSGLTVDSLHPIDLTKPADVRKVVDGLIQRHGRIDVLVNAGAINPQFAAVP
                     DMGYEDIWVPTMVGEVDLVFLMCKAVWPHLVTAGGGSIINFASVAALRGTHFAGMVAH
                     SAGKGAVLAMTRQLAVEGAPHGIRANTISPGLIATMATASQGLTEGEASRRIAASIPL
                     GRIGKPSDIAYCALYLAADESGWVTGINISVDGGTTAA"
     gene            6000..6500
                     /locus_tag="VGE92_10115"
     CDS             6000..6500
                     /locus_tag="VGE92_10115"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HEY1314223.1"
                     /translation="MLRPFADWLADTSLSKILQNLSWVVPTSQSLHILAVSIVFSSAC
                     MINLRLMGVGRSGRSVSQLSATLVPWMWRGLAVLLFTGLIQTVTEPVRQFVTPMFWAK
                     MLMIVVVASMTAVYAQAVRRNAALWDAAATRPARAKAFAVLSTLLWLAIIFCGRFIGY
                     TWSFYA"
     gene            6497..6997
                     /locus_tag="VGE92_10120"
     CDS             6497..6997
                     /locus_tag="VGE92_10120"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HEY1314224.1"
                     /translation="MNIAGLLDNIESWWISAAMRGEVPGTEWLFPIVETLHVMAFAIV
                     FGSIAMLDLRLLGVTSRHNPVSRLSHEVLPWTWTAWCLAAIFGTLMFMAKAQTYAGNL
                     QFRLKFLCMGLAALNMLIFHFGAYRHVSRWDSGEPPMSAKAAGALSLSLWIGVVFFGR
                     WVGFTT"
     gene            7003..7767
                     /locus_tag="VGE92_10125"
     CDS             7003..7767
                     /locus_tag="VGE92_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY1314225.1"
                     /translation="MQFPVPTATVPGLRRNAWLWGLAASVVLLAGLIYALHVRSLEAR
                     LVREWPAELAHSPPLIRIAVWLARPLYAEHCASCHGADLGGNRALGAPNLADPVWLYG
                     DGSIGDIENTILYGIRSGHPKAHNVTDMPAEGRTHQLSAPEIEDVVDFVLALGGQAHD
                     ATAAERGRKLFYDRGNCFDCHASDALGNPDYGSPALLGKTWNYGGDRLTLRQTVYSGR
                     HGLCPAWIRKLTPLQTRALAVYLYVESHPDARLARQ"
     gene            7789..8787
                     /locus_tag="VGE92_10130"
     CDS             7789..8787
                     /locus_tag="VGE92_10130"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY1314226.1"
                     /translation="MMHQVQQPPERFESGDQSAADFFDVSKPQVRRSMTFFGLGALLG
                     LAIAGYGLFTAQGTRSHSVPPEALALVNGRPILRSDFITQVQTQLATPFAQSAAAERE
                     KVLQDMLAEELQVQRGLEIDLPSFDPDVRTALVAGVQLEVTADVLAQQPTAQQLRDYY
                     AAHKGTYLSEGAMRMRDLVAKRETVPGTEPAMANARLAVTALRAGVPLDAAMRRYRLA
                     DSGDLMAAGHVDTGEIFEFAAKAKLPAPVYAVAATLQSGQVSDPIQQGDGAHVVVMLE
                     HRLPVQEDYAAAADKVWTDYKNDAQTRVREANVRYLRSRADILLSADALALESQTK"
     gene            8877..9983
                     /locus_tag="VGE92_10135"
     CDS             8877..9983
                     /locus_tag="VGE92_10135"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEY1314227.1"
                     /translation="MWQIAGNEVHLSFTMPLLESSRLAQSGEEQPPDARVLDYLRQHL
                     SVSSSERLCAVRQGRALAATLQFRRFELTFTCASATDIKLHSSAFFEIVPSHVTFAQI
                     ETADGQFIEQLFTQDHRQVDASSGDARLRAAGFTEYIRLGILHILTGPDHMCFLLGLV
                     LISRRLRDLIFVVTGFTIGHSITLALAVTGILRPHAEYIDALVALTIAVIGAENIAVA
                     THRPGAVAIGIGVPLFAMAAASAAGIGTLPPLLLLGAGLFCANYLMLSGHLRDAGRLR
                     LVVTLVFGLIHGFGFAATLLEMQLPPAKLAQILVGFNLGVELGQISLVLTLVGLVSLL
                     ARAKLALPRPIVVDVVAGSLVAVGTYWFVGRSYV"
     gene            complement(10078..10500)
                     /locus_tag="VGE92_10140"
     CDS             complement(10078..10500)
                     /locus_tag="VGE92_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314228.1"
                     /translation="MTIKSQLRNNAIAIISLVVALSSFSYTAWRTERSERNRTTRQAA
                     FQMLVALGEMKQVVYHGHYDHDEVRGNPRTGWVYVGTIQDFSASMPAPVPAKAHVLMQ
                     RWQQHWEGIGTVDADADAVTDAIDDCRAAVVETIRTLR"
     gene            10755..11384
                     /locus_tag="VGE92_10145"
     CDS             10755..11384
                     /locus_tag="VGE92_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314229.1"
                     /translation="MQRPDAVVSLRSEAPAAIESRALGTLAYIRASIESSSSLDVPGM
                     AGIVMGVIGLLAAILVSLPRGAPHWLGIWLIAAAAALALGSALIARQIAERGHARYWG
                     PARKFVLCLCPALLAGGVLTLVLSGAGSVAFIPGMWLLLYGCAVLSASTVTTAAVARL
                     VGIMGAVFVALGSITFALPLAAHNGMLGLGFGGVHILFGLLIGRVSHVR"
     gene            11374..11775
                     /locus_tag="VGE92_10150"
     CDS             11374..11775
                     /locus_tag="VGE92_10150"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEY1314230.1"
                     /translation="MSGKSGAIKRSETAVAASTKSHAKPAAVPRSPHLNEDAGLDRLI
                     YERVRLGIMSALAMNEQLSFNELKSLFDVSDGNLSAHARKLEEAGYVVCTKSFEARRP
                     KSEYRITQVGKKALLRYLEHIEAVIKATRRA"
     gene            11780..12472
                     /gene="uppS"
                     /locus_tag="VGE92_10155"
     CDS             11780..12472
                     /gene="uppS"
                     /locus_tag="VGE92_10155"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEY1314231.1"
                     /translation="MHVAIIMDGNGRWATDRGLPRTAGHIEGAKAVRTVVEVAARAEV
                     KTLTLYAFSSANWSRPPAEVAALMRLFGQYLFTETRRCVEQSIRINVIGRRDRLSENL
                     LRSIEQSERSSASGSGMLLRIAVDYSSQHSILEAARRVDRDAGLTAADFHRLLQEVNH
                     CAYPASEVDLLIRTGSERRLSDFLLWESAFAELHFSDCLWPDFDEERFRRALEDYSGR
                     QRRFGGLTAKTL"
     gene            complement(12469..13992)
                     /gene="lnt"
                     /locus_tag="VGE92_10160"
     CDS             complement(12469..13992)
                     /gene="lnt"
                     /locus_tag="VGE92_10160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEY1314232.1"
                     /translation="MNGRGPSAALPMQVLMRLAAAFAVGASLNLAFAPFGWWPLAVLA
                     PATLFALIRGLPPRRAAWTGAAFGGGLFVFGTYWLYTCLHVFGLVPIWLTMILQGALV
                     AMMALYSAALCFLANRFWLKAGATRAWLVLPVLWVLLEWLRGWLLSGFPWLSLGYAMI
                     DSPLKGWAPLFGIYGVTWAAAMIAAALNTLAMPDVPAARRLSALGGIVLLAALPVFIG
                     RHAWTRAAGPAVAVAAVQGAVPQDQKWQAKNLPQTMQRYTDLNERAWGAKLIVWPESA
                     LPVLANEIPNYLRRLALQGRAHGADFAIGLVAYQPESNQFRNGILVMSAAGDGWYYKR
                     HLVPFGEYFPVPQFVRSWIRLMSLPYSDFTPGDDRQPTLSAAGQKLGLTICYEDAFGS
                     QQLKASRQATLLINVTNNAWFGDSAAPHQHFQIARMRALEVGRYLVRAANDGITAVIG
                     PQGEIVARLPQFQEAVLRADVRSMTGLTPYARLGNYPVVGGASLLLLLAMWRRRQGV"
     gene            complement(13995..14864)
                     /locus_tag="VGE92_10165"
     CDS             complement(13995..14864)
                     /locus_tag="VGE92_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020798742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEY1314233.1"
                     /translation="MSEQHQSGATGRWLKRITQSMSGEPRDLAELIENLREASDRGLF
                     DGDALVMLEGVLAVADMQVRDIMVPRSQMVFVERDETPEKLIEMVVESGHSRFPVIGE
                     DRDQILGILLAKDLLRLQAAGDESYEIREYMRPVVFVPESKRLNVLLKEFRRSHNHIA
                     MVVDEYGGVCGLVTIEDVIEQIVGEIDDEHDVEDDQTIRRDSAREFTVRALTPIADFN
                     QYFGTAYSDEEYDTIGGLLMQEFGRLPRRGETTKVGEIEFRILRADRRRIDLLRVITP
                     TDIEPPAADESAP"
     gene            complement(14885..15340)
                     /gene="ybeY"
                     /locus_tag="VGE92_10170"
     CDS             complement(14885..15340)
                     /gene="ybeY"
                     /locus_tag="VGE92_10170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEY1314234.1"
                     /translation="MAQRLELDVSYAARRPWVPSSRRFARWAAAALAPLRRPAVLSIR
                     VVGRARSRSLNAQYRGKDKATNVLSFDGAGEVPDGRCFLGELVICAPVVAHEALAHGK
                     TLDAHWAHMTVHGVLHLLGFDHEADSEAVIMAAREVQILDRLGVSDPYR"
     gene            complement(15345..16322)
                     /locus_tag="VGE92_10175"
     CDS             complement(15345..16322)
                     /locus_tag="VGE92_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY1314235.1"
                     /translation="MSSISTTRDVLLAPADNVRLVELCGPLDEHLRQIEARLGVEMRR
                     RGNRFQIIGLPAAVKRAESVLLDLYTRAQREPVDSERVHIALQELDMDSNNGDAKADS
                     DELRVHTARGAVRARGANQTEYLNNVRTHDLTFGIGPAGTGKTYLAVACAVEALQSEK
                     VRRIVLVRPAVEAGERLGFLPGDLSQKVDPYLRPMYDALYEMMGFERVAKLIERNVIE
                     VAPLAFMRGRSLNDSFVILDEAQNTTNEQMKMFLTRIGFGSKAVVTGDITQVDLPNSK
                     QSGLRTVIDILRGVRGIAFTLFSSRDVVRHPLVQRIVQAYEASAPDDGP"
     gene            complement(16319..17668)
                     /gene="miaB"
                     /locus_tag="VGE92_10180"
     CDS             complement(16319..17668)
                     /gene="miaB"
                     /locus_tag="VGE92_10180"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEY1314236.1"
                     /translation="MPGLLYIKTFGCQMNEYDSGKMRDVLEAAHGMARTDNPAEADVL
                     LVNTCSVREKAQEKVFSLLGEWRLFKQQRPNVIIGVGGCVASQEGEGITARAPFVDLV
                     FGPQTLHRLPAMLEGLRQTGRSQIDISFPEIEKFDRLPQPRAGGATAFVSIMEGCSKY
                     CSFCVVPYTRGEEISRPFDQVLAEVERLAGQGVREITLLGQNVNAYAGPMADGTLADL
                     GLLIHFVAEVPGVERIRFTTSHPLDFNDSLIEAYANVPKLNNFLHLPVQSGSDRVLAA
                     MKRGHTVLEYKQKLRKLKAVRPDICISTDLIIGFPGETDQDFIATMNLVADVGFDQSF
                     SFIYSRRPGTPAAALPDEVAHAVKQQRLERLQQRLNAQARAISERMLGSRQRVLVERP
                     AKRNPQQLAGRTDNNRWVNFDGPAQNPHALIGNFVDVVITEAMPNSLRGRIVADGAP"
     gene            17718..18251
                     /gene="def"
                     /locus_tag="VGE92_10185"
     CDS             17718..18251
                     /gene="def"
                     /locus_tag="VGE92_10185"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY1314237.1"
                     /translation="MAIREVLKMGDPRLLALAQPVHEFGTPELESLLADMRDTMRDLN
                     GAGLAAPQIGVGLRVVIFGFEANPRYPDADAVPFTVLVNPTLTPLGEQQDDGWEGCLS
                     VPGMRGLVPRYRDLRYTGFDEHGSPIDRTVKDFHARVVQHEVDHLDGILYPRRIRDLT
                     QFGFNDALFPGEKLTDD"
     gene            18301..18762
                     /locus_tag="VGE92_10190"
     CDS             18301..18762
                     /locus_tag="VGE92_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY1314238.1"
                     /translation="MSTTFELNGKPATLDGDPKMPLLWAVRELAGLPGTKFGCGMALC
                     GACTVQLDGKAIRSCVTPLASVAGRKVTTIEGLSSAPAKALQTAWVKLQVPQCGYCQS
                     GQIMSATALLESNSTPTDADINSAMSGNICRCATYSRIRAAIHAAADSIKA"
     gene            18766..20952
                     /locus_tag="VGE92_10195"
     CDS             18766..20952
                     /locus_tag="VGE92_10195"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY1314239.1"
                     /translation="MSTTVPAARAHRRDFLKGAASLAVLTIGFDWGGPTRRALAASTP
                     IAGATFAPNAFLRIGADNSVTVIAKHLEMGQGAYTGIATVLAEELDAGWAQVRVESAP
                     ADAKRYANLVFGTMQGTGGSSAMANSWMQLREAGGKARAMLLAAAAKEWNVPVAELTV
                     DQGSVHHAAKKREATFGALVPTAVTLPVPGSVQLKDPKQFKLIGQRAPRVDAAAKSDG
                     TAQFTLDVVLPGMLVALLKRPPLFGATVKSFDGTAASAVPGVVKIVQVPRGVAVVARS
                     FWAAKLGRDVLQVQWDDSKAEKRSSAALMDEYRRLAEQPALSALKRGDALQALKDAPH
                     KVAASFEFPYLAHAPMEPLDAVVKLDAKSCEIWAGDQFQTVDQANAAHTAGLDPQQVS
                     IHTLYAGGSFGRRANPGSDYIVEAVSIAKAYGADGTPIKLQWTREDDIHGGLYRPMYY
                     HKLEAGLDAAGTLSGWRHVIVGQSIVADTPFAAMIKDGIDPTSVEGAASIAYSIPNIA
                     VDLATTKTGVPVLWWRVVGSSHTTFAVEAFIDEVAHAAGQDGFTFRRKLLEHEPRMKA
                     VLELAADKAGWNTPLPPGKGRGIAVAEAFKTFVAQVAEVSVGKDGQVKVERVICAVDC
                     GTPINPDIIAAQMEGGIGFGLGAVLYGAITLKDGHIEQDNFNGYRVLRINEMPKVEVH
                     IVPSTAPPTGVGEPGVAPLGPAVANAIYAATGKRTYVLPFAKAGTA"
     gene            complement(20990..22288)
                     /locus_tag="VGE92_10200"
     CDS             complement(20990..22288)
                     /locus_tag="VGE92_10200"
                     /inference="COORDINATES: protein motif:HMM:NF023330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HEY1314240.1"
                     /translation="MRPQDHPLRSELNDEIHARPSESLSAPMRLSYLALASPSEAREE
                     EWRHVLELVKTIGMEISERPVNHYSACLGDFSLRWERHAEFTRYTFWAPSSGDIFASS
                     VLDLLPAGWVLRIPGRVLVATHGGIVKDTEQVDALNIAAVRWFDGNVLVGSSVGDGAA
                     IAVTDFRVRSDGFSRFVIFDHTLTPRQAGRTVQRLLEIDSYRMMALLALPIARELAPI
                     IHQAESELSSCMTRLEKAHQSDESMLLDSLTHLGARIERWDAQHSYRFSAAAAYYELV
                     QRRIAELREVRIAGLQTFGEFTERRLAPAMNTCLTIARRQESIALRIARANQLLTTRV
                     DISVERQNQALLESMNRRAKAQFKLQQTVEGLSVAAITYYVVGLIGYGAKALHARGYK
                     VDYELVEGLSIPVVLLLIAAGTYFLKHRVKRYLHPAADKT"
     gene            complement(22329..>23621)
                     /locus_tag="VGE92_10205"
     CDS             complement(22329..>23621)
                     /locus_tag="VGE92_10205"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ndvB"
                     /protein_id="HEY1314241.1"
                     /translation="AAGRQFAAGDVQHWWLPLSGQGVQTRVSDDRIWLPFVVAHYLQV
                     TEDVAVLDEAAPFLAGEPLADGADERFWTPHAGQPATLYAHCVLALDSSLAVGSHGLP
                     LFGTGDWNDGMNRVGAKGRGESVWLAWFLHAALMRFAPIADQRGDSSAATRWRKHAFA
                     LQRAVEREAWDGDWYRRGYFDDGTPLGSVSSDECRIDSIAQSWAVISGAADSERAARA
                     MSAVDRQLVNRSEGLVQLFTPPFYRNSHDPGYIMAYPRGIRENGGQYTHAAMWTVLAF
                     ALQGDGDRAAELFALINPINHGSRSGIHRYKVEPYVACADVYTAPSHVGRGGWTWYTG
                     SAAWMYRTAVEAILGVNLRGKILSIDPCIPRTWSAFEILYRHGATRYRIRIENPRSVS
                     RGVASLTLDGEEIAPASRGIPLIDDGGEHNCLVTLGRD"
BASE COUNT         4300 a         7517 c         7290 g         4514 t
ORIGIN      
        1 cccgcctcga ccggcacggc aagcaccaca gccggcgcca acaccgggat cgcggcgtac
       61 gccgatcgcg gcatcatcgc ggcactcaag tcgctcgtcc aggatctcgg caacagtcag
      121 ttgatcagca gcacggactc ctcgtcgagt ctctcggcca acaccctatc gaatctcaat
      181 tcggcattcg agaagttgat tggcgatctg ggcggcagca cctcgacctc cggcggcact
      241 tcaagcaccg cgggcagtca gtcgaccgcg gcgctgcagt cgttcttgac aggtttctta
      301 caagacttgc aaaacaacgg cacccaatcc ttgagcgcgt tgggctcgag cgtcaacacc
      361 accgcctgat cagaggcgcg gcggtggttc gcgcgccgcc caaatgagat gatggcagga
      421 gaaagcactc ccgcctcgtc aaatcttggg attttgtatg gcaaccgtgc ccgacgtcgg
      481 aggcggtgcg gacgcgactc cgcagcgggt caacaatcgc tctctcaaga tccggcaaac
      541 gattacggtg ctggcgttat tcggtggcta tgcggcgctc tacttctgcc gcgccgactt
      601 ggcggtcgcg acgccccttc tcatcgaaga actgggcaag catggcgtca gtcacgccga
      661 tgccatcctt cgcatgggcg agatcacctc cgtgggtgtg ctcgcctacg cagtgggcaa
      721 gctgttcctg accggcctcg gcgacttttg gggtggccgt atcaattttc tgatcgggct
      781 gctcggcgcc acgcttttca ccttgctgtt tgcgtctcgc ttgagcctgc cgctgttcag
      841 catcgcctgg atcggcaatc gcctgactca atcgatcgcc tgggcgggtc tcgtcaaagt
      901 atcttccaag tggttcgact tttcctccta cgggatgatc atcggcatct tgagcatcag
      961 ctacctcgtc ggcgatgcct tggcgcggga ctggatgggg attctgatcg aacacggctt
     1021 cggctggcgc gcgttgtttt atttcgcggc ggcggtcggc gcgttgttct tggccgtcaa
     1081 ttatttcttt ctgcgcgaat cgcgcatcga tgccggcttc acggaggcca agccaaaccc
     1141 gctcaacctg ttcgccgggt ctgaagcccc gccgcagagc ctcggccagc tgctgctgcc
     1201 gctgctgcgc agccatgcat tcttgttggt atgcctgttg tcgctcggct gtaccatcat
     1261 tcgcgaaaca ttcaatgcat ggacacccgt gtatctacgc gactatctcg gttacagcat
     1321 gggcgatgcc gcgcgcatga gcggcatttt cccgggtgtc ggcgccgtat cggtgctggt
     1381 caccggctgg ctcagcgatc gcctcggcgt gaacgggcgc tcgctcatca tgttcgtggg
     1441 attggcagca acggctgccg cgttgctgct gctcatgacc atgcattcca gcttgaccgg
     1501 atcgctgctg ccattgatcg ccatcggcat catcgccttt tgcctgctcg gcccctactc
     1561 ctacctcggc ggggctttcg cgctcgattt cggcggcaag caggcgggcg ccgtctcttc
     1621 gggcatcatc gacggcgtcg ggtatctcgg ggcggtggcc gccggctggg ctgtcgcacg
     1681 cgtcgcggtg tccttcggtt ggcaaggcgt attcgtcgcc ctcgccctgg tgagcgcctt
     1741 gtcggcactc ggtgccgggt gtctgtactt gctgggtgcg aaggcggcgg cgcaccatac
     1801 ccacctgcca tgaccgtcgc cgacgtgaag cgtgtctcgt tcagccccga ccggctccac
     1861 gtcgaatggg ccgacggcag cttaagcgaa ttcgccagtc tctggctgcg cgacaatctg
     1921 cgcgaggatc gcgatcctca cagcgggcag cggctgatcg atatcgcgga tctaccccag
     1981 agtcctaaga ttcgcgccgc gacggcgcac aacggctgcg tgagcctcga ttgggcggat
     2041 ggcgaacgca ccgcgacctt cgaactcgac tggttgctgg cacactcttc cagccacggc
     2101 ggccgacgcc gagggcgcgc gcaccgcctg tggctcgagg cagcgggcct cgcggcgacg
     2161 cgcgatttcg cttgggctgt ctgcaacgaa ctcgccggcc accgtgcggc gcgcgcgcac
     2221 tggctgaccc ggttgttgca ggatggcctc ggctttctga gcgacgtgcc gcgcaacgat
     2281 gcgggaattc tcgaagctgc gagactcgcc gggcgcgtcg cggagaccaa ctatggcctg
     2341 gtcttcgagg taaggtcggt cgcacagccg gaaaatttgg cctactccga tttgggcttg
     2401 ggcctgcata ccgataaccc ctatcgcgaa cccgtgccgg gatttcaaat gttgcacgtg
     2461 ctcatcgcat ctcccgacgg cggcgagagc ctgtttggcg atggctttgc cattgccgag
     2521 catctgcgca gcacggaccg cgaggcattt gcggtcctca cgcaaacggc tgtgcctttt
     2581 ctgtaccgct cgaaagatgc ggaactttat gccgagaggc ctctgattca gttgtcctgc
     2641 gacggcgagg tgaccgccgt gcactacaac agccgctcga tcgcgccgct gcagcgcgac
     2701 gcgcgcgagt gcgaacggtt ctaccgcgcg taccgccgct tcgcctcgct gctgcgcgat
     2761 ccgcgcttcc agctgcgatt catgctgcgc gacggggatc tcgcggtgtt cgacaatcaa
     2821 cgcatcctgc atggccgcac ggcattttcg tcgtcgcgct tcgcgcgaca cttgcggggc
     2881 tgctacctga cacgcgatag cgtgtattct caagctgcgc tgctgcggcg cgaatttccc
     2941 gtggatgagc cgtcatgagc cttgccgatg aactatttgc gatttttcac gaccgcggcg
     3001 ctgccgagta tttcggcgaa cgtgtgtcgg tgaccgaaca tgccttgcaa gcggcttatt
     3061 tcgcacagat cgcttccgcg cctccggcct tggtcatcgc ggcgctgctg catgatatcg
     3121 ggcacctcgt cgagcaggtg ccgagcgata ttgccgactg gaagacggat gcgcggcacg
     3181 aagtcgtcgg cggcgagtgg ctcgcgaaac gattcggtcc cgaagtctcc gaaccggtgc
     3241 gcttgcacgt acccgccaag cgctatctgt tggcgaccga tgatgcctac ataacccagt
     3301 tgagccccgc ctcgctggtc actctgaagc tgcaaggcgg cccgatggcg gcgcatgaaa
     3361 tcgctcaatt cgaggccgag cgctattacg ccgacgcagt ccgcgtgcgc cgctgcgatg
     3421 accaaggcaa ggtggcggga atgaagactc cggacttctg cgactatcgc gcgttgatcg
     3481 atgcattagg tcgctgatcc ccggggcgcg cgccgccatg aaacccaaat atttcgcgac
     3541 cgcgcaaagc ctgcgcgctt ggctgcacgc gaacgcggcc gcgagcgccg aactcatcgt
     3601 cggattctat aagcgagaca gcggcacggc gagcgtcacg tggccgcagg cggtggatga
     3661 ggcgctgtgt gtcgggtggg tggatggggt acgccatcga atcgatgagc ggcgctacaa
     3721 aattcgattc acgccgagaa agccgagctc gatctggagt gcgataaaca tcgagcgtgt
     3781 gcgcgtgctc ggcgccgaag gacgcatgca gccggccggt ctcgcagcct tcgggcgtcg
     3841 catcgagaaa aactctcgaa tttattccta cgagcagcca ggtctcgccg ctctgacagc
     3901 gcaggaacaa gcaacgttca agcgcaacaa agccgcatgg gcgtttttcg acgcccagcc
     3961 cctgggctac aagaaaagga tgctctggcg aatcgtcagc gccaagcagc cgccaacccg
     4021 cgagcgccgg tttgccgctt tgctcgaggc ctcggcgcaa gggacgcggc tgtagggcgc
     4081 ttcgactgct ccaccggcct gtctggtctg gagcgcaccg cgtgcggatg gtcggtcgac
     4141 ctcgatgttg ataatccggc agtattcacg taaattacgt gaattgctgc cagaacctat
     4201 ctccatgaca gtcaagcaca atatcaccgt tgcgatcgag cccgggttgc tcaagaaggc
     4261 ccgagcggtt gcggcgcgtc gagggcgcag cgtcagcgcc ctgctggcag acgagctccg
     4321 tgaattggtg gcggaggacg ccctgtacac caaggcgcgc aaacgtgcgc tcgcgctgtt
     4381 ttcggctcct ttgccactcg gaggtactcc gctagcgcgt gaggagctcc atgaccggcg
     4441 tcgtcttcgt tgattccaat atccttatct acgcgcacga cgcagacgcc ggcgcgaagc
     4501 gatcgcgtgc ggcagataag cttcaggaac tgtgggaatc cggcggcggt cggctctcgg
     4561 tgcaagtgct gcaagagttc ttcgtcaacg taacgaggaa gctctccacg cccgttgctc
     4621 gatcaactgc acgcgaggtc gtgagcaatt acggcacgtg gatccgtgag ccgacgacag
     4681 ccgccaccgt catacgggct attgatattg ccgagatggc tcagattcaa ttctgggatg
     4741 cattgatcgt cgcctccgct gaacaggcag acgctacccg aatatatagc gaggatctga
     4801 acaatggaca gacgatcgcg ggagttcaga tagtcaaccc gttgctcaaa acgtaggcgc
     4861 cctcgatcct cgaccagcct tagggcactg actcgtcggg tattggcggt aggtgctgca
     4921 gcgcctcgcg ccgaagttgc tccaagtacg cattccacgt ctcagccaac accgccgcgc
     4981 gcgcggccgc atacaaacgc cgagcacggc cagcacggcg acacccagcg cggcggcgac
     5041 ccaccgcgga cgatgcttgc cgttcatccg gcaattctcc tacgcggcgg tcgtgccacc
     5101 atcaacgctg atattgattc ccgtgaccca acctgattcg tccgcggcta gatatagcgc
     5161 gcaatacgca atgtcggacg gcttcccgat gcgtccgagc gggattgaag cggctattcg
     5221 ccggctcgcc tccccttcgg tgagtccctg tgaggccgtc gccatggtgg caatcaatcc
     5281 cggagaaatc gtgttcgcgc gaataccatg cggcgcgccc tcaaccgcga gctgtcgggt
     5341 catcgccagt acagcgccct tgcccgcgct gtgcgcgacc atgcccgcaa aatgggtgcc
     5401 acgcaaggca gccaccgacg caaaattgat aatggaccca ccgccggccg tcaccaaatg
     5461 cggccacaca gctttgcaca tcaagaatac gaggtcgacc tcaccgacca tcgtcggtac
     5521 ccagatgtcc tcatagccca tatctggcac ggccgcgaat tgcggattga tcgcacccgc
     5581 attgacgagc acatcgatac ggccgtgacg ctgaatcagt ccgtcgacga cttttcggac
     5641 atctgcgggc tttgtcagat caatcgggtg cagactatcc acggtcaggc cgctgagttt
     5701 cgccgcatct accgtggagc gcgcacgaga cgcgtcgatg tcgcatccca tgacgacggc
     5761 cccttctcgg gcaaacatca atgcgcattc gcgcccgata cccgcaccaa tgccggtcac
     5821 cagagccact ttcccagaca gcctagccat gcatgttcga cccgtcgaat cctacgcccg
     5881 cgactctacg accagcccct gtagtttgcc atcataaatg caatgcaggt gcgccgcgtt
     5941 cgaatcggcg tgctcgatat aatcacccct gatcgtcgtg aggtcatcgc gaggttccca
     6001 tgttgcgtcc gtttgctgac tggcttgccg atacatcgct gagcaagatt ctgcagaatc
     6061 tgtcgtgggt ggtacccaca tcgcagtcgc tgcacatctt ggcggtatcg atcgtcttta
     6121 gctccgcctg catgatcaat ctgcggctga tgggagtggg acggtctgga cgcagcgtct
     6181 cgcagctgag cgcgaccttg gtgccgtgga tgtggcgcgg actcgctgtg ctcttgttca
     6241 ccggcctgat tcagaccgtg acggagccgg tgcggcaatt tgtcacgcct atgttttggg
     6301 ccaagatgct gatgattgtc gttgtggcaa gcatgacggc ggtctacgcg caggcggtgc
     6361 gccgcaatgc ggcgctctgg gatgctgcag cgacgcgacc cgcgcgtgcc aaggcctttg
     6421 cggtactgtc cacgctgctc tggctggcga tcatcttttg cggccgcttc atcggctaca
     6481 cctggtcgtt ctacgcgtga atatcgcggg gctgctcgac aacatagaga gctggtggat
     6541 ttccgccgca atgcgcggcg aggtgcccgg cacggagtgg ctatttccga tcgtcgaaac
     6601 gctgcacgtc atggcctttg ccatcgtatt cggctcgatt gcgatgctcg acctgcgatt
     6661 gctgggtgtc acttcgcgcc acaatcccgt ctcgcgactg tcgcacgagg tgctgccatg
     6721 gacctggacc gcgtggtgcc tcgcggccat attcggtacc ctgatgttca tggccaaggc
     6781 gcagacctat gcggggaatc tacagtttcg gcttaaattt ttatgcatgg ggctggccgc
     6841 actgaacatg ctcatttttc acttcggggc ctatcggcac gtttcgcgct gggattcagg
     6901 cgagccaccg atgagcgcga aggcggccgg cgcgctgtct ctgtcgctgt ggatcggcgt
     6961 ggtgttcttc ggacgctggg tcggttttac gacctgagat ccatgcagtt ccccgtaccc
     7021 accgctacag tgccgggctt gcgccgcaac gcctggctct ggggcctggc ggcatcggtc
     7081 gtgctgctcg ccggacttat ttatgctctg catgtgcgtt cgctggaggc gaggctggtg
     7141 cgcgaatggc ctgcggaatt ggcccacagc ccgccgttga tacgtattgc ggtgtggctg
     7201 gcccggccgt tatatgctga gcattgcgct tcctgtcacg gcgccgacct cggcggcaat
     7261 cgcgcgctcg gcgcgcccaa tctggccgat ccagtgtggc tctacggtga cggtagcatc
     7321 ggcgatattg agaatacgat tctctacggt atccgctccg gacatcccaa agctcacaac
     7381 gtgaccgata tgccggccga gggccgcacg caccaattgt cagcgcccga gatcgaggac
     7441 gtcgttgatt tcgtgctcgc cttgggtgga caagcgcacg atgcaacggc ggccgaacgc
     7501 ggccgcaagc ttttttatga ccgcggcaat tgttttgatt gccacgcgag cgatgccttg
     7561 ggcaacccgg actatggcag cccggccttg ctcggtaaga cctggaatta tggcggcgat
     7621 cggctgacac tgcggcagac cgtttacagc ggccgtcacg gtctgtgccc ggcctggata
     7681 cgcaaactga ctcccctgca gactcgcgca ctcgcggtat atctctacgt ggaatcgcac
     7741 ccggacgcca ggctcgcacg gcagtaaggc cgacaaagcg gatccaatat gatgcaccag
     7801 gtacaacagc cccccgagcg gttcgaatcg ggtgaccaat ccgccgccga cttctttgat
     7861 gtgtcgaagc cgcaggtccg gcgctcgatg acttttttcg gcctcggggc actgctcgga
     7921 ttggcgattg cgggctatgg gctgttcacc gcccaaggca cccggtcgca ttccgtaccg
     7981 ccggaggcgc tcgcactcgt caacggccgg ccgattctgc gcagcgattt tatcacgcag
     8041 gtgcagacgc agttggcaac gccgtttgca cagagcgctg cggcggaacg cgaaaaagtc
     8101 ttgcaagaca tgctggcgga ggagttgcag gtacaacgag ggctcgaaat agatctaccg
     8161 agtttcgatc ccgacgtgcg cacggccttg gtggccggcg tccaactcga ggttaccgct
     8221 gacgtgctcg cgcagcaacc gaccgcgcag cagctgcgcg actactacgc ggcacataag
     8281 ggaacgtacc tcagcgaggg ggcaatgcgc atgcgtgact tggttgccaa acgcgaaacc
     8341 gttcccggga cagaaccggc gatggcgaat gcgaggctgg cggtgacggc gcttcgtgcg
     8401 ggtgtgccgc tcgatgcggc catgcggcgc taccggcttg cagactccgg ggacttgatg
     8461 gcggccggtc acgtcgatac gggcgagatt ttcgagtttg cggcgaaagc gaaactccct
     8521 gcgccggtgt atgcagtcgc ggccactctg cagagcggtc aagtatcgga tccgatccaa
     8581 caaggcgatg gcgcgcacgt ggtcgtgatg ctggaacatc gcttgccggt gcaggaagac
     8641 tatgccgctg ccgccgacaa agtgtggacc gattacaaga acgacgccca aacacgggtg
     8701 cgcgaggcca atgtgcgcta tctgcgcagc cgcgccgaca tcctattgtc tgctgatgca
     8761 cttgcgctcg agtcgcaaac gaagtgaggg cccgggtgcc gctctgctgt tgcttctggt
     8821 tgctcgcgct atggagcgcg tgcgcgtccg cacatactaa gagcgagacg cattcagtgt
     8881 ggcagatcgc cggcaatgag gtgcatctgt cattcaccat gccgctgctc gagtccagtc
     8941 ggctggcgca gtccggtgaa gagcagccac ctgacgcacg cgtgctcgac tacctgcgac
     9001 aacatctatc agtcagtagt agcgagcggc tttgcgccgt tcggcagggg cgtgcgctgg
     9061 ccgcgacgct gcagtttcgt cgtttcgaat tgacctttac ctgcgccagc gcgaccgaca
     9121 tcaagctcca ttcctcagcc ttcttcgaaa tagtgccatc gcacgttacg tttgcgcaga
     9181 tcgagaccgc ggacggtcag ttcatcgagc agttgttcac ccaggatcat cggcaggtcg
     9241 atgcatcgtc cggcgatgcg cggctgcgcg cggccggctt cacagagtat atccgtctcg
     9301 ggatcctgca catcctgacc ggcccggacc atatgtgctt tttgttgggg ttggtgttga
     9361 tttcgcgacg cctgcgcgat ctgatattcg tagtcaccgg cttcacgatt ggccacagca
     9421 tcactctggc gctcgccgtc accgggattt tgcgccctca cgcagaatat atcgatgcgc
     9481 tggtggccct gacgatagcc gtgatcggtg ccgaaaatat cgccgtcgcg acacaccggc
     9541 ctggcgcggt ggcgataggc attggcgtgc cgctattcgc catggcggcg gccagcgctg
     9601 caggcatcgg cacgctgccg ccgctgctgt tgctcggcgc gggcctgttc tgtgcaaact
     9661 acctgatgct ctccgggcat ttgcgcgacg cgggccgatt gcgcctggtg gtcacactcg
     9721 tattcggtct gattcatggt tttggcttcg ctgccacgtt gctcgagatg cagctgccac
     9781 ccgccaagct cgcgcagatc ctggtcggat ttaatctcgg cgtcgagctc ggccagatct
     9841 cgctggtgtt gacactggta ggcttggtga gcctactggc gcgcgccaag ctcgcgttgc
     9901 cgcgaccgat cgtggtcgat gtcgtcgccg gatccttggt ggccgttggc acctattggt
     9961 tcgtcggtcg cagctacgtc tgatggcaag gcaatcgcta atgttccagc aactcgaaca
    10021 cgtgttgcgg ctgtgcacta gccatcgcga gatccaatgc cgtggcaact cacgttttca
    10081 gcgcagcgtg cggatagtct ccactaccgc ggcgcgacag tcgtcgatcg cgtcggtcac
    10141 tgcatcggcg tccgcgtcca ccgttcctat cccttcccag tgctgctgcc acctttgcat
    10201 cagcacgtgc gccttggctg gcaccggtgc cggcattgat gcgctaaagt cctggatcgt
    10261 tccgacgtaa acccacccgg tgcgcggatt gccgcgcacc tcgtcatggt cgtaatgacc
    10321 gtgatagacg acctgcttca tttcaccgag agcgacgagc atctgaaacg cagcttgacg
    10381 cgtcgtgcga ttacgttcgc tgcgctcggt gcgccacgcc gtgtaactga agctcgacaa
    10441 ggccacaacc aaactgatga tggcgattgc gttgttgcgt aactgagatt ttatcgtcac
    10501 tgtcaaattg gttccgcaag gctcgagccc ttgcaactcg ctaccgcaaa tttgtctatc
    10561 atacgacgcc gaccgtcgga ccaaccgctt cgggtggtcg cgtgctgctg aattcgtggc
    10621 acggcgctat tcttcacgct catcgcccac gagactgagc atggcattga cgcggcaaat
    10681 ctgtgacgaa gttcacctaa ctttgcgctt tagagtactt gacgcaacac agtacgctcc
    10741 cttaagctga tttcatgcaa aggcccgacg cggtagtctc tcttcgatcc gaagcgccgg
    10801 cggccattga gagccgcgcg ctcggtaccc tcgcctacat acgcgcgtcc attgagtcct
    10861 cgagttcgct ggatgtgccc ggcatggccg gcatcgtcat gggggtcatc ggcctgctgg
    10921 cggcgatcct ggtgtcgttg ccgcgcgggg cgccgcattg gctcggcatt tggctgattg
    10981 ccgcggcggc cgcacttgcg ctcggcagcg ctttgatagc tcggcagatc gccgaacgcg
    11041 gtcacgcgcg ctactggggg ccggccagaa aattcgtcct ctgtctgtgt cccgcgctgc
    11101 tcgccggcgg cgtgctcaca ttggttttat cgggcgccgg tagtgtggcg ttcatacccg
    11161 gcatgtggtt gcttctctac ggttgtgcgg tgttgtcggc aagcaccgtg accacggccg
    11221 ctgttgcgcg gctcgtcggc atcatgggag ccgtgttcgt cgcgctcgga tcgatcacct
    11281 tcgccctgcc cttggcggcg cataatggca tgctcggtct gggattcggc ggcgtgcaca
    11341 tcctcttcgg tctgctgatc ggacgcgtgt cccatgtccg gtaagtccgg cgccatcaag
    11401 cgcagcgaaa cggccgttgc ggcgtcgacc aagagccacg ccaagccggc cgcggtcccg
    11461 cgttcgccgc acctgaacga agatgcggga cttgaccgcc tgatctatga gcgcgtgcgt
    11521 ctcggcatca tgagcgctct cgccatgaac gagcaattga gcttcaacga actcaagtcg
    11581 ctgttcgatg tcagcgacgg caacttgagc gctcacgcgc gcaaactcga ggaagcgggc
    11641 tacgtggtgt gcaccaaatc cttcgaggcc cgccggccaa aatcggagta ccggatcacg
    11701 caagtgggga agaaggccct gttgcgctat ctcgagcaca tcgaagccgt catcaaagct
    11761 acgcggcgcg cttaaggtca tgcacgtcgc aatcatcatg gatggaaacg gacgctgggc
    11821 gaccgaccgt ggcttgccgc gcaccgccgg gcatatcgag ggcgcgaaag cggtgcgcac
    11881 ggtggtcgag gttgcggcgc gtgcggaggt caagacgctg acgctgtacg ccttctcctc
    11941 ggctaactgg tcgcgcccac cggccgaggt cgcggcgctg atgcgcctgt tcggccagta
    12001 tctatttacc gagactcgcc gctgcgtcga acagtcgatt cgaatcaacg tcatcggccg
    12061 acgcgatcgg ctgagcgaga atttgctgcg ctccatcgag cagagcgaac gctcgagcgc
    12121 aagcggctcc gggatgctgc tgcgcatcgc cgtcgactac tcatcgcagc acagcatctt
    12181 ggaagccgcg cggcgcgtcg atcgcgacgc ggggctgacc gcggcggatt ttcaccgcct
    12241 gctgcaggag gtcaatcact gtgcctatcc ggcgagcgaa gtcgacctct tgatccgcac
    12301 cgggagcgag cgtcggctca gcgattttct gctctgggaa tccgcgtttg ccgagctgca
    12361 cttcagcgat tgcctgtggc cggacttcga cgaggagcgg tttcggcgtg cgctcgagga
    12421 ttacagcggc cggcagcggc gcttcggcgg cttgaccgca aaaacgcttt aaacgccctg
    12481 ccgccgccgc cacatcgcga gcagcaacaa tagactcgcg ccgccgacca ccggataatt
    12541 gccgagcctc gcataggggg tgagacccgt catcgaccgc acgtcggcgc gcagcaccgc
    12601 ctcttggaac tgcggcaggc gcgccacgat ctcaccttga gggccgatta ccgcggtgat
    12661 gccgtcgttc gcggctcgca ccaaatagcg ccccacctcg agcgcgcgca tccgggcaat
    12721 ctgaaaatgc tgatgcggcg ccgccgaatc gccgaaccag gcattgttcg tgacattgat
    12781 caacaaggtc gcctggcgcg acgccttgag ttgctgactg ccgaaggcgt cctcatagca
    12841 aatggtcagg ccaagcttct ggccggcggc actcaacgtc ggctggcgat catcgccggg
    12901 cgtgaagtcg gaatacggca gactcatcag ccgtatccac gagcgcacga attgcggcac
    12961 cggaaagtat tcgccgaacg gcaccaagtg ccgcttgtaa taccagccgt cgcccgctgc
    13021 gctcatcacc aggatgccgt tgcgaaactg attggattcc ggctgatagg cgacgagccc
    13081 gatcgcaaag tccgcgccat gcgcccgccc ctgcagcgcc aatcgccgta ggtaattggg
    13141 tatttcgttg gcgagtaccg gcagtgcgga ttccggccag acgatgagct tggcgcccca
    13201 cgcccgctcg ttgaggtctg tgtagcgttg catggtctgc ggaagattct tcgcttgcca
    13261 cttctggtcc tgcggcaccg cgccctgcac cgcggccacc gccacggcgg gcccggcggc
    13321 gcgcgtccat gcatggcggc cgatgaacac cgggagcgcc gccaacagca cgatgccgcc
    13381 gagcgcgctg aggcggcgcg ccgccggcac atcgggcatc gccagcgtat tcaacgccgc
    13441 cgcgatcatg gcggctgccc acgtcacgcc atagattccg aacaggggcg cccagccctt
    13501 gaggggcgaa tcgatcatcg cgtagccgag cgacagccac gggaacccgc tcaacaacca
    13561 gccgcgcaac cattcgagca agacccaaag taccggcagc accagccagg cgcgcgtcgc
    13621 gccggctttc agccaaaagc gattggcaag aaagcacaga gccgccgaat acagcgccat
    13681 catcgcgacc agcgcccctt gcaggatcat cgtcagccag atcggcacca gaccaaacac
    13741 gtgcaggcac gtgtacagcc agtaagtgcc gaatacgaac aacccaccgc cgaacgcagc
    13801 gccggtccat gcggcgcggc gcggcggcaa gccgcgaatc agggcgaaca gggtggcagg
    13861 cgccaacacc gcgagcggcc accagccgaa cggcgcaaac gcgagattga gactggcgcc
    13921 gacggcaaac gccgccgcca ggcgcatcag cacctgcatc ggcagcgcgg ctgacgggcc
    13981 gcgtccgttc acgctcaagg agccgactcg tcggcggcgg gaggctcgat atcggtgggt
    14041 gtgatcacac gcaataaatc gatgcggcgg cgatcggcgc gcaaaatgcg aaactcgatc
    14101 tcgccgacct tggtggtctc cccgcggcgc ggcaggcggc caaactcttg catcagcagg
    14161 ccgccgatcg tgtcgtattc ctcgtcggaa taggcggtgc cgaaatactg gttgaagtcg
    14221 gcgatcggcg tgagcgcgcg caccgtgaat tcacgcgcgc tgtcccgccg aatcgtctgg
    14281 tcgtcttcga cgtcgtgctc atcgtcgatc tcgccgacga tctgttcgat gacatcctcg
    14341 atggtgacca ggccgcacac gccgccatat tcgtcgacca ccatggcaat atgattatgg
    14401 ctgcgtcgaa attccttcag cagcacgttg aggcgcttgc tctcgggcac gaacaccacc
    14461 ggtcgcatgt actcgcgaat ctcgtaactc tcgtcgcccg ccgcttgcag ccgcagtaag
    14521 tccttcgcga gcaggatgcc gagaatttga tcgcgatcct cgccgatcac cgggaagcgc
    14581 gaatgcccgg attcgaccac catttcgatc agcttttcgg gcgtttcgtc gcgctcgacg
    14641 aacaccattt gcgagcgcgg caccatgatg tcgcgcacct gcatgtccgc gaccgccaat
    14701 actccctcga gcatgaccag cgcgtcgccg tcgaacaggc cgcggtcgct cgcctcgcgc
    14761 aaattctcga tgagctcggc gagatcgcgc ggttcgccgg acatcgactg ggtaatgcgt
    14821 tttaaccagc ggccggtggc gccgctttgg tgttgttctg acatgggggc ttaaggatac
    14881 tcgatcagcg atacgggtca gaaaccccaa gccgatcaag gatttgaacc tccctcgcgg
    14941 ccattatgac cgcctcggag tcggcctcgt ggtcgaatcc gagcaggtgc agtacgccgt
    15001 gtacggtcat gtgtgcccaa tgcgcgtcga gggtctttcc gtgggcaagg gcttcgtgcg
    15061 ccaccaccgg ggcgcaaatg acgagctcgc ccaaaaaaca ccggccatcg ggcacctcac
    15121 cggccccgtc gaatgacaga acattggttg ccttgtcctt gccgcggtac tgcgcgttga
    15181 ggctgcggct gcgcgcccgg cccaccacgc gaatcgacag caccgccgga cggcgcagcg
    15241 gcgcgagcgc cgccgcggcc caacgggcga agcgccgcga actcgggacc cagggtcgac
    15301 gcgccgcgta actaacatcg agttcgagcc gctgcgccac tgcctcaggg accgtcgtcg
    15361 ggggcgctcg cctcgtaggc ttgcacgatt ctctgcacca gcgggtggcg caccacgtcg
    15421 cgcgagctga acagggtgaa tgcgatgccg cgcacgcccc gcaaaatatc gatcacggtg
    15481 cgcaggcccg attgcttgga attcggcagg tcgacctggg tgatgtcgcc ggtgaccacc
    15541 gccttcgagc cgaagccgat gcgggtcagg aacattttca tctgctcatt ggtcgtgttc
    15601 tgcgcctcgt cgagaatcac gaacgagtcg ttgagcgagc ggccgcgcat gaacgcgagc
    15661 ggcgccactt cgatgacgtt gcgttcgatt aatttcgcca cgcgttcgaa gcccatcatt
    15721 tcatagagcg catcgtacat tgggcgcaag tacggatcga ctttttgcga cagatcgccg
    15781 ggcaaaaacc ccaggcgttc gccggcctcg accgcggggc gaaccagcac gatgcgtctg
    15841 actttttccg attgcagcgc ctccaccgcg caggcgacgg cgagataggt cttgcccgtg
    15901 ccggcggggc cgataccaaa cgtcaggtcg tgcgtgcgca cgttgttgag gtattcggtc
    15961 tgattggcgc cgcgggcacg caccgcaccg cgcgccgtgt gcaccctgag ttcatccgaa
    16021 tccgctttgg cgtcgccgtt gttgctgtcc atatccagtt cttgcagcgc gatgtgaact
    16081 cgttcggaat ccacgggctc gcgctgcgcc cgcgtgtaca agtcgagcag caccgactcg
    16141 gcgcgcttca ccgcggccgg caggccgatg atttgaaatc gattgccccg cctgcgcatt
    16201 tccacgccga gtcgcgcctc gatctggcgc agatgttcat cgagcggtcc gcacagttcc
    16261 accaagcgga cgttgtccgc gggcgcgagc aatacatcac gcgtcgtgga aattgaactc
    16321 acggagcccc gtcggcaacg atccgtccgc gcagcgaatt cggcatcgct tccgtgatga
    16381 cgacatcgac gaagtttccg atgagagcat gcggattctg cgcggggcca tcgaaattga
    16441 cccagcgatt gttgtcggtg cgcccggcca gctgctgggg attgcgcttc gcggggcgct
    16501 cgaccaggac gcgttgccgg ctgcccagca tgcgctcgga gatggcgcgc gcctgggcgt
    16561 tcaagcgctg ttgcaaccgt tcgagccgct gctgcttgac ggcatgcgcc acctcgtccg
    16621 gcagcgccgc tgccggggtg cccgggcggc ggctgtagat gaagctgaac gattgatcga
    16681 accccacgtc ggccacgaga ttcatcgtag cgataaaatc ctggtccgtc tcgcccggaa
    16741 atccgatgat cagatcggtt gagatgcaaa tatccggccg caccgccttg agcttgcgca
    16801 gcttttgttt gtactcgagc acggtatggc cgcgcttcat ggccgcgaga acgcgatccg
    16861 aaccgctctg caccggcaag tgcaggaagt tattgagctt cggtacgttg gcgtaggcct
    16921 cgatcaagct gtcgttgaag tcgagcggat gcgaggtggt gaagcgaatg cgttccacgc
    16981 cggggacctc ggcgacgaaa tgaatcagca accctaaatc ggcgagcgta ccgtccgcca
    17041 tgggtcccgc gtaggcgttc acattctggc cgagcagcgt gatttcgcgc accccctgcc
    17101 cggctaaacg ctcgacttcc gcgagcacct gatcgaaagg gcggctgatt tcttcgccgc
    17161 gcgtataggg cacgacgcaa aaactgcaat acttgctgca gccttccatg atcgacacga
    17221 aagcggttgc gccgccggcg cgcggctgcg gcagccggtc gaatttctcg atttcgggaa
    17281 agctgatgtc gatttgcgat cggccggttt gcctgagtcc ctcgagcatc gccggcaagc
    17341 ggtgcagggt ttgcggaccg aacaccaggt cgacgaacgg cgcccgagcg gtgatgcctt
    17401 cgccctcctg gctcgcgacg cagcccccca ccccgatgat cacgttcggc cgctgctgct
    17461 tgaacaggcg ccattcgccc agcagcgaaa acaccttctc ctgagccttc tcgcgcaccg
    17521 agcaggtatt gaccagcagc acatcggcct ccgccggatt gtcggtccgc gccatgccat
    17581 gggcggcctc gagcacgtcc cgcatcttgc cggagtcgta ctcattcatc tgacaaccga
    17641 aggtttttat gtataaaaga ccgggcatac gttgggcata ggctcacata aaaaaggccg
    17701 ctaagataca aggaaccatg gcaatacgcg aagttctcaa aatgggcgat ccgcgcctgc
    17761 tggcgctggc ccaaccggtg cacgaattcg gcaccccgga gctcgaaagc ctgctggccg
    17821 atatgcggga cacgatgcgc gatctcaatg gggcggggct cgctgcaccg caaatcgggg
    17881 tcggcctgcg cgtggtgatt ttcggcttcg aggccaatcc gcgctacccg gacgcggatg
    17941 cggtgccctt caccgtgctg gtcaatccga ccttgacgcc gctcggggag cagcaggacg
    18001 acggctggga gggatgccta tccgtccccg gcatgcgcgg gctcgtaccg cgctatcgcg
    18061 atttgcgcta caccggcttc gatgaacacg gcagccccat cgaccgcacg gtgaaggatt
    18121 ttcacgcgcg ggtagtgcag cacgaggtcg atcacctgga cggaatcctg tacccgcgcc
    18181 gcatccgcga tctgacccaa ttcggcttca atgacgctct gtttcccggc gagaagctca
    18241 ccgacgactg agcgtcgagg atgatctaga atgacgggca ttcacggagg cgcttaaatc
    18301 atgtcgacga cattcgaact caacggcaaa cccgcaacgc tcgacggcga cccgaagatg
    18361 ccgctgctgt gggcggtgcg cgagttggcc ggcctgcccg ggaccaagtt tggctgcggc
    18421 atggcgttgt gcggcgcctg taccgtgcaa ctcgacggca aggcaatccg ctcctgcgtg
    18481 acgccgctcg ccagcgttgc cgggcgcaag gtcacgacca tcgagggctt gagcagcgcg
    18541 ccggcgaagg cgctgcaaac ggcgtgggtc aagcttcagg ttccgcaatg cggatactgc
    18601 caatccggcc agatcatgtc ggcgactgca ctgctcgaat cgaattccac gccgaccgat
    18661 gccgacatca atagcgccat gagcggcaac atttgccgct gcgccaccta ctcgcgtatc
    18721 cgtgcggcga ttcatgccgc ggccgactcc atcaaggcat aatttatgag cacgaccgtt
    18781 cccgccgcac gcgcacaccg ccgcgatttc ctcaagggag ccgcgtcgct tgccgtactg
    18841 acgattggct tcgactgggg cggcccaacg cgccgcgcgc tcgctgcctc caccccgatc
    18901 gcgggcgcaa ccttcgcgcc caatgccttc ctgaggatcg gtgccgacaa cagcgtcacg
    18961 gtgatcgcca agcacttgga aatgggccag ggtgcctaca ccggcatcgc caccgtgctc
    19021 gccgaggaac tcgacgccgg ctgggcccag gttcgtgtcg aaagcgcgcc cgccgatgcg
    19081 aagcgctatg ccaatctggt gttcggtacg atgcaaggaa ccggcggcag ctctgcgatg
    19141 gcgaattcct ggatgcagtt gcgcgaagcc ggtggcaaag cccgcgccat gctgctcgcg
    19201 gcggcagcga aggagtggaa cgtgccggtt gccgaattga ctgtggacca gggtagcgtg
    19261 caccacgctg ccaagaaacg cgaggccacc ttcggtgcgc tggtgcccac tgccgtcaca
    19321 ctgcccgtgc ccggcagcgt acagctcaag gatcccaagc aattcaaatt gatcggacaa
    19381 cgcgcgccgc gcgtggacgc agctgcgaaa tccgacggca cggcccagtt cacgctcgat
    19441 gtcgtattgc ccggaatgct ggtcgcgctg ctcaagcggc cgccgttgtt tggcgcaacg
    19501 gtcaaatcct tcgatggcac ggcggcgtct gccgttcccg gcgtcgtcaa gattgtgcaa
    19561 gtgccgcgcg gtgtcgcggt ggtggcaaga agtttctggg ccgcgaagct agggcgcgac
    19621 gtgctgcagg tgcaatggga cgactccaag gccgagaagc gcagctccgc ggcattgatg
    19681 gacgagtatc gccggctcgc ggaacaaccc gccctctcgg ccctgaaacg cggcgatgcg
    19741 ctgcaggcgc tcaaggacgc cccccacaaa gtcgccgcga gcttcgagtt cccctacctc
    19801 gcgcacgcac cgatggaacc gctcgacgcg gtggtcaagc tcgatgcgaa aagctgcgag
    19861 atctgggccg gagatcaatt tcaaaccgtg gaccaggcga acgccgcgca cacggcgggc
    19921 ctcgatccgc agcaagtcag cattcacacg ctgtatgccg gcggcagctt cggccgccgc
    19981 gccaaccccg gttccgacta catcgtcgaa gcggtgtcga ttgccaaggc ctacggcgcc
    20041 gacggcacgc cgatcaaact gcagtggacg cgcgaggacg acatccatgg cggcctgtat
    20101 cggcccatgt attaccacaa gctcgaagcg ggcttggacg ccgcgggcac gctcagcggc
    20161 tggcggcatg tcatcgtcgg ccagtcgatc gtcgcggata cgccgtttgc cgcgatgatc
    20221 aaggatggca ttgatccgac gtcggtcgaa ggtgctgcga gcatcgccta ttccattccg
    20281 aacatcgcgg tcgatctcgc gaccacgaag accggcgtgc cggtgctgtg gtggcgcgtg
    20341 gtcggcagtt cgcacaccac gttcgccgtc gaagccttca tcgatgaggt ggcgcacgcc
    20401 gccggccaag acggcttcac atttcgacgc aagctgctcg agcatgagcc gcgcatgaag
    20461 gcggtgctgg agctggcggc ggacaaggcc ggctggaaca ccccgctgcc gccgggcaaa
    20521 ggccggggta tcgcggtcgc cgaagccttc aagaccttcg tcgcacaggt agccgaggtc
    20581 agcgtcggca aggacggtca ggtcaaggtc gagcgggtga tttgcgcggt cgattgcggc
    20641 acgccgatca atcccgacat cattgccgcg caaatggaag gcggcatcgg ctttggcttg
    20701 ggcgcggtgc tctatggcgc gatcaccttg aaggatggcc acatcgagca ggacaacttc
    20761 aacggttatc gcgtcttgcg cataaacgaa atgcccaagg tcgaggtgca catcgtcccg
    20821 tcgaccgcgc cgcccaccgg cgtgggcgag cccggcgtcg cacccttggg tcccgcggtc
    20881 gccaacgcca tctacgccgc caccggcaag cgcacctatg tgttgccgtt cgccaaggcc
    20941 gggaccgcgt agcaggccgc tgtcggcacg cttatagttg aggctcgacc tatgtcttat
    21001 ccgcggccgg atgaagatat cgcttcactc gatgcttgag gaagtaagtg cccgcggcga
    21061 tgagcagcag caccaccgga atgctcaagc cttcgaccag ttcgtagtcg accttatagc
    21121 cccgggcatg taatgccttc gcaccgtagc caatcaatcc cactacgtag taggtaatgg
    21181 ccgccaccga aagcccttcg accgtttgct gcaacttgaa ctgcgccttt gcgcgccgat
    21241 tcatggattc gagcagcgcc tgattctggc gttcgacgga aatgtcgacc cgcgtcgtga
    21301 gcagttggtt cgcccgcgca attcgcaggg ctatggactc ttggcggcgc gcgatcgtga
    21361 ggcacgtgtt catggccggt gccaaacgcc gctcggtaaa ttcaccgaag gtttgcaatc
    21421 ccgcgatgcg tacttcgcga agttcggcga ttcgacgttg caccaactcg taatacgccg
    21481 cagcggcgct gaatcgatag ctgtgttgag catcccaccg ctcgatgcgt gcgcctaaat
    21541 gagtgaggct gtcgagtagc atcgattcgt cactttgatg agctttttcg agacgggtca
    21601 tacatgaact caactccgac tctgcctgat ggatgatggg ggccagttct cgcgcgatcg
    21661 gcagcgccag cagggccatc atgcgataag aatcgatctc aagcaggcgt tgcacggtgc
    21721 gtcccgcctg acgcggcgtc aatgtgtggt cgaagatcac gaaccggctg aacccgtcgc
    21781 tgcgcactcg aaaatccgtt acggcgattg cggccccatc gccaaccgag gaaccaacaa
    21841 ggacatttcc gtcaaaccaa cgaaccgccg cgatattgag ggcgtcaact tgctctgtgt
    21901 ctttcactat gccgccgtgc gtcgccacca agacacgccc tggaatgcgc agaacccacc
    21961 ccgccggtaa caggtcgagg acggacgagg caaatatgtc gccgctgctg ggtgcccaga
    22021 aggtgtagcg tgtgaattct gcatgtcgtt cccaccgcaa ggaaaaatcg ccgagacagg
    22081 cgctgtaatg attcacaggc cgctcggaga tctccatgcc gatggtcttt accaactcga
    22141 ggacatgccg ccattcctct tcccgcgcct cgctcggaga tgccaatgcg agataactca
    22201 agcgcatagg ggcgctcaag gactccgatg gtcgcgcatg tatttcgtcg ttcagttccg
    22261 agcgcagagg gtgatcttgc ggacgcatcc atgagagtct acccgtctta cgataaacgc
    22321 cctagctttc agtcgcggcc cagcgtgacc agacagttgt gttcaccgcc atcgtcgatc
    22381 agcggaattc cgcggcttgc aggggcaatc tcctcgccat ccaatgtcag agacgcgacg
    22441 ccgcgcgaga cgcttcgagg attctcgata cggattcgat agcgtgttgc tccgtgcctg
    22501 tagagaatct cgaacgcgct ccaagtgcgc ggaatacacg gatcgatcga caggatcttg
    22561 ccgcgcaggt tcacaccgag gatcgcctcg acggcggttc gatacatcca agcagccgag
    22621 cccgtgtacc acgtccagcc gccgcgcccg acgtgcgacg gcgccgtgta aacgtcggcg
    22681 cacgcgacgt aaggttccac cttgtagcga tgaatgccgg agcggctgcc atgattgatc
    22741 ggattgatga gagcgaacaa ttcagcggcg cgatccccat ctccctgcaa tgcaaacgcc
    22801 aacaccgtcc acatcgcggc gtgggtgtac tgcccgccgt tttcccggat accgcgtgga
    22861 tacgccatga tgtagccggg gtcgtgggag tttctataga agggcggcgt gaacaactgc
    22921 accaaacctt cgctgcggtt cacgagctga cggtcaacgg ccgacatcgc gcgcgccgcc
    22981 cgctcggaat ccgcggcgcc ggaaatcacg gcccacgact gggcaatcga atcaatccgg
    23041 cactcgtcgc tcgataccga gccgagcggt gtaccgtcat cgaagtatcc gcggcggtac
    23101 cagtcgccgt cccaggcctc gcgctcgacg gctctttgca gtgcgaatgc gtgcttgcgc
    23161 catcgggtcg cggcggaact gtcgccgcgc tgatcggcga tcggtgcaaa acgcatgagt
    23221 gccgcatgca ggaaccaggc gagccacacg ctctcgccgc ggcccttcgc gcccacgcgg
    23281 ttcatgccgt cgttccaatc gccggtgccg aacaacggca agccgtggga gccgaccgcc
    23341 aagctcgagt cgagtgccag tacgcaatgc gcatacagcg tcgccggttg ccctgcgtgc
    23401 ggcgtccaga agcgctcgtc ggcaccgtcc gcgagcggct cgcctgcgag gaacggagct
    23461 gcttcgtcca agacggcgac atcttccgtc acttgaagat aatgagccac cacgaacggc
    23521 agccatattc ggtcgtcgga cacccgggtc tggacgccct ggccggacag gggaagccac
    23581 caatgctgga cgtccccggc cgcaaattga cgtccggcgg c
//
LOCUS       DASWZT010000363        14967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_7643, whole genome shotgun sequence.
ACCESSION   DASWZT010000363 DASWZT010000000
VERSION     DASWZT010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14967
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7643"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1343)
                     /locus_tag="VGE92_10210"
     CDS             complement(<1..1343)
                     /locus_tag="VGE92_10210"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1314242.1"
                     /translation="MRKLLLLSVASIPLANIAASAADFEDDPDTVVVTATRIPTPELE
                     VASSISVVTADQIADRQIQTLPDLLKEVPGLNVVQAGGPGGQTSVFMRGTNSNHTKVL
                     VDGIDVSDPSSPVGTFDFGQFLAQDIQKVEILRGPQSGLYGSDAIGGVINVITKSGSG
                     PAQFNAAVEGGSFDTFNQTVGLSGSLERFHYAANIEHYHSGESPVTPLDLLAPGERRN
                     DDYFDNLTGSTKLGFDVTDDFDLGLVARYTNSHLRITGENEDNFPVDFPDATQSENDT
                     HQYYARATAHLKSFADALEQTLGVAYSNIKSFETGPDGPESDFFGARVKIDWQGVIRL
                     AADEKLVLGAEHQRDEMTVPISASTTIDSGYAELQSSFADSLFNTISVRYDDNDRFGG
                     KVTYRVAPAYVIPDTGTKFKASVGTGFKAPTLSQMFQNFPDFNFYANPDLKPESSV"
     regulatory      complement(1489..1677)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1698..2534)
                     /gene="fghA"
                     /locus_tag="VGE92_10215"
     CDS             complement(1698..2534)
                     /gene="fghA"
                     /locus_tag="VGE92_10215"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEY1314243.1"
                     /translation="MTLVSEHGCFGGVQRFYKHDSNEIRGPMKFSAYLPPQARSGKVP
                     VLYYLSGLTCTEETFPIKAHAQQAAAELGLMLIAPDTSPREPRLPGDADSWDFGYSAG
                     FYVDATVAPWSQYYRMYSYVTRELPQVIGANLPVEAGASGIFGHSMGGHGALICALKN
                     PDRYKSVSAFAPIAAPMQCPWGKKAFSNYLGPDPQAWAQYDASELVARKPFPGPILID
                     QGTADQFLVEQLLPEKFSAAAAKSGQVLTLRMQPGYDHGYYFMQTFMAEHLRHHAKQL
                     KN"
     gene            complement(2543..3652)
                     /locus_tag="VGE92_10220"
     CDS             complement(2543..3652)
                     /locus_tag="VGE92_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEY1314244.1"
                     /translation="MKTKAAVATAARKPLEILSVDLEGPKAGEVLVEIKASGVCHTDE
                     FTRSGADPEGLFPAILGHEGAGIVVDVGPGVTTLRKGDHVIPLYTPECRQCKSCTSRK
                     TNLCTAIRATQGKGLMPDGTSRFAIGKDMVHHYMGCSTFSNFTVLPEIALAKIREDAP
                     FDKVCYIGCGVTTGIGAVINTAKVEAGANVVVFGLGGIGLNVVQGARMVGANKIIGVD
                     LNPGRKALAEKFGLTHFVNPKEVQGDLVAHLVALTDGGADYSFECVGNVDLMRQALEC
                     CHRGWGVSVVVGVAGAGKEISTRPFQLVTGRVWKGTAFGGARGRTDVPKIVDWYMDGK
                     INIDDLITHKMPLTDINKAFDLMHSGESIRSVVVY"
     gene            3718..4392
                     /locus_tag="VGE92_10225"
     CDS             3718..4392
                     /locus_tag="VGE92_10225"
                     /inference="COORDINATES: protein motif:HMM:NF016240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HEY1314245.1"
                     /translation="MNIEFSANEARVIGCLIEKEITTPDQYPLSLNALTNACNQKTNR
                     EPVLELSEATVQQAVDSLMKKYMVSDKSAGYGGRVTKYKHRFCNTEFGSLKFSKQELG
                     ILCVLLLRGPQTPGELRARTNRLCEFTDADQVEATLRNLMTREDGPFVARLPRAAGAR
                     ESRYAHLFSGNIESVEETAPLEEPSVAGAPTLSQRVADLEESVRRLRLELDALSAAKQ
                     GATTGG"
     gene            complement(4379..5077)
                     /locus_tag="VGE92_10230"
     CDS             complement(4379..5077)
                     /locus_tag="VGE92_10230"
                     /inference="COORDINATES: protein motif:HMM:NF012725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1314246.1"
                     /translation="MLLLAWCAAGPQLACGQGYPGGGMQGGGMPGGRGGMQSAPGDEI
                     PNTPAPEKPDKAAKKAFQAGVKSLNRAREYTDIAAKATNPDKKADALDKVSDAYGRAL
                     DQFTEALSNKGDMVEAWNNVGYIHLQLGAYGEAIDDYNHTLALDPNLLEAVEHRAEAH
                     MAVDRLDDAQAAYMSLFNHSRPLADQLLVTMQKWLESHRLAANGMRPKDIEAFGKWLD
                     ERETIAKQTAANPQ"
     gene            complement(5294..5746)
                     /locus_tag="VGE92_10235"
     CDS             complement(5294..5746)
                     /locus_tag="VGE92_10235"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HEY1314247.1"
                     /translation="MNAVDIAHLIQIALTPIFLISAIGITLNVLTNRLARIVDRARAI
                     EDEVRESERTHEARDLHETLRVLARRARYINSAITLITLSALFIALVVVMLFVNAFLR
                     WDLSVFIASMFILSMLTLAGALTAFLIEVRIATNTLRIGIEAASRHGE"
     gene            complement(5743..6195)
                     /locus_tag="VGE92_10240"
     CDS             complement(5743..6195)
                     /locus_tag="VGE92_10240"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEY1314248.1"
                     /translation="MRHKNQRVCGAALLALWLFLAPAPAAEFRAGALTVGGAWSPPTP
                     SVATVGAAYFSITNSGRDTDRLLAISTPIARQVEIHESRTVQGTIQMRAVAWIECPPG
                     VTKIEPGGVHVMLLGLSTPLAAGMQFPLNLQFRDAGTLRIQVQVGARE"
     gene            6311..7909
                     /locus_tag="VGE92_10245"
     CDS             6311..7909
                     /locus_tag="VGE92_10245"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HEY1314249.1"
                     /translation="MSKYPWTVAAALSLPMLAFASSHREAPNISRLPTLDGTDFYMFN
                     SYESGRAGYVTLIANYIPLQDPYGGPNYFALDPAGLYEIEIDNNGDAKEDLTFQFQFT
                     NTLANKNAGITLPVGPAASPATVAVPLKNVGPISASSSANLNFIETYTVTLVTGDRRS
                     GVATPVTNAANGSATFVKPMDYIGQKSFGSDAGYAAYAAQYMYTINVPGCSSPGRVFV
                     GQRKEGFAVNLGEVFDLVNFVPIEGDSSPGAGDGKGFPGGITQNPSHNVLANKNITSI
                     ALELPAACLKGSGNGVIGGWTTASLRQARILDPNATFSKPEVNGGAWTQVSRLGMPLV
                     NEVVIGLPDKDHFNSSSPINDAQFATYVTNPSLPALLNALFLAPVNATLGTSIANLAP
                     NNFPRNDLVAAFLTGFAGLNQQATVTASEMLRLNTAVAATPATAQSFFGVAGGDLAGF
                     PNGRRPGDNVVDIALRVVMGALCYPLPIGANKAPTNLGLCTPANAPVGNVPFTDGAPV
                     NALDFDQTFPYLKTPLPGSPIGSM"
     gene            7922..8182
                     /locus_tag="VGE92_10250"
     CDS             7922..8182
                     /locus_tag="VGE92_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314250.1"
                     /translation="MFYTSVRWRLAAGAMLAAVVGCSGGGGGSPAMTTPTPPATQQST
                     DFSSLVYMIYGQPANSQPVSLDNLTIVYDVNDDPTDFNGLLM"
     gene            complement(8216..9073)
                     /locus_tag="VGE92_10255"
     CDS             complement(8216..9073)
                     /locus_tag="VGE92_10255"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEY1314251.1"
                     /translation="MPFVIKIVILAIVQGFCELLPVSSSAHVIIAEKLMGLDPTTPAM
                     TFLLVMLHTGTMLAVIAYFWNTWRSRYFSSASAFWSFARSLILATVCTAIIGFGLIVF
                     IEHVILRGAVKAEVELLFGNLTLIAVALATVGVLIIVSGLRSAAKARQSVEFRDAVWI
                     GMVQGLCLPFRGFSRSGATISTGLLRGLDKELLENYSFALAVILTPPVIAREGYRLLG
                     AHAAAGLTSGSTGLIWPSVMGVALSFIAGWLSLKWLSRWLEHGRWHLFGYYCLFAAVV
                     VFAVGKSGF"
     gene            complement(9138..9341)
                     /locus_tag="VGE92_10260"
     CDS             complement(9138..9341)
                     /locus_tag="VGE92_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314252.1"
                     /translation="MNKSGTLITTALLTLGLAAAAMSADMPKVAAPMAADTPAAGTAT
                     ETPKPAKKSHKKHKKAPAPATTK"
     gene            complement(9422..9925)
                     /locus_tag="VGE92_10265"
     CDS             complement(9422..9925)
                     /locus_tag="VGE92_10265"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HEY1314253.1"
                     /translation="MITADRPAHLPRLNVLRTIALYKLVKVLLLLAIAYGEVRLSDAS
                     LTAKLLTWASARPLGLEHDVVTWLLEKFSGLSASQVHALRIVTLAYAALFATEGVGLW
                     MQKRWAEWLTTIITASLIPLEVYELLFRPNLGKAVVLIANIAIVIYLAWHVRTRGKHA
                     DSPPGAL"
     gene            complement(9932..10312)
                     /locus_tag="VGE92_10270"
     CDS             complement(9932..10312)
                     /locus_tag="VGE92_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314254.1"
                     /translation="MTETASYLHLHVPPDEMVKYIQDLMTLPHGDIAASSALALAQRH
                     WGGDTPLARAALLRWGALNLAFQDKRLETWTVTRQRDRIQVPAALVAAAGIAPLVLDN
                     ERAVFDIPALLDATLEFQPPAGQA"
     gene            complement(10323..11624)
                     /locus_tag="VGE92_10275"
     CDS             complement(10323..11624)
                     /locus_tag="VGE92_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY1314255.1"
                     /translation="MNSSPLLQCTEVRKAFPKPDGAELLVLEGMNLELREGQIVGLLG
                     RSGSGKSTLLRLIAGLAEPTGGQVCYLGQPIVGPASSIAMVFQSFALFPWLTVFENVA
                     LGLEAQRMARADIRKRSLAAIDLIGLDGFESAYPRELSGGMRQRVGFARALVVHPNIL
                     LMDEPFSALDVLTAETLRTDFLDLWSEGRMPIKGVILVTHNIEEAVLMCDRILVFGSN
                     PGRILSEIKVSLPQPRNRLDPSFRELVERIYVEMTARPQAAGSARHERFPGLGIGSQY
                     PQVSSNLLAGLMEAVAAAPFNGTADLPKIASDLQMEIDELFPVAETLQMLRFAELEGG
                     DLKLTPDGLAFAQAARDERKRIFSRHLLTYVPLAAHIRRVLDERASHSARKSRFIDEL
                     EDFMSEEAADQTLRAVLRWARYAEAFAYDDENAMFSLENPT"
     gene            complement(11635..13353)
                     /locus_tag="VGE92_10280"
     CDS             complement(11635..13353)
                     /locus_tag="VGE92_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY1314256.1"
                     /translation="MNRFAPYVFLRDFWSRWDVLAFIVVIGLIAFLGETSRGLFSPLS
                     TLEMTPLSLSPSHLPEYAARTTFRMLAGLFLSLVFTLTYATWAAKSERAGKLLVPILD
                     ILQSVPILGFISITVVFFMSLAPGRVLGAEFAAIFAIFTSQAWNMAFSFYQSLRTIPT
                     ELAEAAESFRLSPWMRFWQLEVPFGMPALIWNMMMSMSGGWFFVVASESISVGHTTVA
                     LPGVGSYIALAIEQKNLHAIGWAIATMLIVILLYDQLLFRPLVAWVDRFRVEQEPGVR
                     VPDSWALTMMRRSRVILAASLAFHAAVLWTSRAVRRSDKPSVENPMVRKRSLDFLWVT
                     VVAAAVLLGCWHIVLVLIKNTPLHEALQVCGLATLTMLRVFILIALASLVWVPIGVWV
                     GMRPQVAQIVQPVAQFMAAFPANLLFPLAVYGIVAWKLNPDIYLSPLMILGTQWYILF
                     NVIAGASAIPAEMRYVAANFRVRGWLWWRKVALPGILPFYVTGAITASGGSWNAAIVA
                     ELATWGKTQVEARGLGSYIAEATTAGDFHRIVLGIGTMSLFVVIINRLFWRPLYYYAE
                     RKYRLT"
     gene            13480..14649
                     /locus_tag="VGE92_10285"
     CDS             13480..14649
                     /locus_tag="VGE92_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1314257.1"
                     /translation="MPTRFNVLGAISFSHFLNDMMQSLIVAIYPLLKGEFSLSFVQIG
                     LITLTYQICASVLQPLIGIYTDKHPKPYSLSVGMGFTLIGMVALAFAPNYALVLVAAA
                     MIGAGSSIFHPESSRIARLASGGRHGLAQSIFQVGGNAGSAMGPLLAAWIIVPRGQSS
                     LAWFALAALLAIAVLARVGAWYKRQHLERAGAGRRAAVPSPVSARRVAWSIVILVALI
                     FSKYFYIASISSFYSFYLIEKFHLSVRSAQMYLFVFLLAMALGTLFGGPIGDRIGRKR
                     VIWASILGVAPFSLLLPFVGLMWTGILTFIIGLILSSAFSAIVVFAQELMPGKVGAVS
                     GLFFGFAFGIGGIGAAVLGAVADSHGIEFVYRLCAYLPLLGMVAAFLPDIEHHRT"
     gene            14646..>14967
                     /locus_tag="VGE92_10290"
     CDS             14646..>14967
                     /locus_tag="VGE92_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314258.1"
                     /translation="MTHQIILTIGQYGLMIVFVNVLLDQIGLPVPAVPTLIVAGAIAA
                     DGQLPLASLFVWSVIACLAADCGWYLIGERYGIRVLKTLCRISLEPDSCVSQTQTRFE
                     RWGIN"
BASE COUNT         3026 a         4704 c         4381 g         2856 t
ORIGIN      
        1 acgctgctct cgggcttcag atccggattg gcgtagaaat tgaagtcagg gaaattttga
       61 aacatctgcg acagagtcgg cgccttgaag ccggtgccga cgctcgcctt gaacttggtt
      121 cccgtgtctg gaatgacgta cgccggcgcc acgcgatagg tcaccttgcc gccgaaccga
      181 tcgttatcat cgtagcgcac gcttatggtg ttgaatagcg aatcggcgaa actcgattgc
      241 aattccgcat atccggaatc gatggtggtg ctcgcagaga tcggcaccgt catctcatcg
      301 cgctgatgct ccgcgcccag caccagcttt tcatcggcgg cgagccgaat gactccctgc
      361 cagtcgatct tgacgcgcgc gccgaagaaa tcgctttccg gaccatcggg tcccgtctcg
      421 aacgacttga tgttgctgta tgcgacgccg agcgtctgct cgagcgcatc cgcaaacgat
      481 ttgaggtgag cggtggcgcg tgcgtaatac tgatgggtgt cgttttcgct ttgcgtcgcg
      541 tccgggaaat cgacgggaaa gttgtcttcg ttctctccgg tgatccgcaa gtgcgagttg
      601 gtgtagcgcg cgaccaagcc gagatcgaaa tcatcggtca catcgaagcc gagcttggtc
      661 gagccggtga ggttgtcgaa atagtcatcg ttgcgccgct cgccgggtgc gagcagatcc
      721 aacggcgtta ccggcgattc gcccgagtgg taatgctcga tattcgccgc ataatgaaac
      781 cgctcgagcg aaccgctaag gcccacggtc tggttgaacg tatcgaacga gccgccctcg
      841 acggcggcgt tgaattgcgc cggtcccgag ccgctcttgg tgatcacgtt gatcaccccg
      901 ccgatggcgt cggagccgta caagccgctt tgcggaccgc gcaggatctc caccttttga
      961 atgtcctgag cgaggaactg gccaaaatcg aacgtgccga ccgggctgct cggatcgctg
     1021 acgtcgatgc cgtcgaccag cactttggtg tgattggagt tcgtgccgcg catgaacacc
     1081 gaggtttggc cgcccggacc gcctgcctgc acgacgttga gcccgggcac ttccttcaat
     1141 aagtcaggca gcgtttgaat ttgccggtcc gcgatctgat ccgccgtgac gacggaaatg
     1201 ctgctggcaa cctcgagctc cggagtgggg atccgagtgg cggtaaccac caccgtatcc
     1261 ggatcgtctt cgaagtcggc ggccgacgca gcgatgttcg cgagagggat ggatgcgact
     1321 gaaagcagca acaatttgcg cacaaggtac tcctggtttg gaccaagagc cggcacgcga
     1381 aggcacactc atgctgaata ttattttcca acttcatggc tccgcaacga cgactccaca
     1441 agtcgtcgcg acggtttccc ccgcagctat cagcgcaccc cggccaataa caggacgact
     1501 gcgcgaggcg ggtgtcctgg ctcccgggtc ttggcctccg gtccgccttc ccaaggcttg
     1561 atcgcctcag tggattcttg gaccttcgct cgccggttac agttgcgggg gcagccgcgg
     1621 tattgcaccg cgttccgatt taagtccctt gcgggaacct tgcgcgagtc gagatcatac
     1681 tcgttatggc gtcggggtca attcttcagc tgcttggcgt ggtggcgcaa gtgttccgcc
     1741 atgaaggttt gcatgaagta gtaaccgtga tcgtagcccg gctgcatgcg caaagtgagc
     1801 acttggccgg acttggcggc ggccgccgag aatttttcgg gcaatagctg ttcgacgaga
     1861 aattgatcgg cggtgccttg atcgatgagt atcggaccgg gaaagggctt gcgcgcgacc
     1921 agctcactgg catcgtattg cgcccaggcc tgcgggtccg gtcccagata attgctgaat
     1981 gccttcttcc cccaaggaca ttgcatcggc gccgcaatcg gcgcaaacgc ggacaccgat
     2041 ttgtagcggt cgggattctt gagcgcgcat atcagagcac cgtggccgcc catggaatga
     2101 ccgaagattc cgctggcgcc ggcctcaacc gggagattgg cgccgatgac ttgcggcagt
     2161 tcgcgcgtga cgtagctgta catgcgatag tactgcgacc agggagcgac ggtggcatcg
     2221 acgtagaacc ccgcggaata accgaaatcc caactatcgg catcgcccgg cagtcgcggc
     2281 tcgcgcggac tcgtgtccgg cgcgatcagc atcaatccca attccgcggc cgcctgctgc
     2341 gcgtgagctt tgatgggaaa ggtttcctcg gtgcaggtaa gccccgacaa gtagtacagc
     2401 accggtacct taccggaacg cgcctgcggc ggcagatagg cggagaactt catcggtccg
     2461 cgaatctcgt tcgagtcgtg cttgtagaag cgctgtacgc cgccgaagca gccgtgttcc
     2521 gaaaccaacg tcaaggcgcc catcagtaca ccaccacgct gcgaatcgat tcgccggaat
     2581 gcatcaaatc gaaggctttg ttgatatcgg tgagcggcat tttgtgagtg atgagatcgt
     2641 cgatgttgat cttgccgtcc atgtaccaat cgacgatctt gggcacgtcg gtgcgcccgc
     2701 gtgcaccgcc gaacgcggtt cctttccaga cgcgcccggt cacgagttga aacggccggg
     2761 tgctgatctc cttgccggcg ccggcgacgc cgaccaccac cgacacgccc cagccgcgat
     2821 ggcagcactc gagcgcctgg cgcatcagat cgacgttgcc gacgcactcg aaactatagt
     2881 cggcgccgcc gtcggttagc gccaccaagt gagcgaccag gtcgccctgg acttctttcg
     2941 ggttgacgaa atgcgtcagt ccgaatttct cggcgagtgc cttgcggccc ggattcaaat
     3001 ccacgccgat gatcttattc gcacccacca tgcgcgcgcc ttgcacgaca ttcagaccga
     3061 ttccgccgag gccaaacacc accacattcg cgccggcctc caccttggcc gtattgatga
     3121 ccgcgccgat cccggtggtc acgccgcaac cgatgtagca caccttgtcg aacggcgcgt
     3181 cctcgcgaat ctttgccaga gcgatctcgg gcagcaccgt aaaattcgag aacgtcgaac
     3241 accccatgta gtgatgcacc atgtccttgc cgatggcaaa gcggctggtg ccgtccggca
     3301 tcaagccctt gccctgcgtg gcgcggatcg cagtgcagag attggtcttg cgcgaggtgc
     3361 acgatttgca ctgccggcac tccggagtgt acagcggaat gacgtgatcg cccttgcgca
     3421 gcgtcgtcac gcccggaccg acgtcgacca cgatgcccgc cccctcgtgt cccaaaatcg
     3481 cggggaataa cccctccgga tccgctcccg aacgtgtaaa ttcgtccgtg tgacatactc
     3541 cactggcctt gatttccacc agcacctcgc ccgctttggg cccttccaaa tcgacgctga
     3601 ggatttccag gggtttgcgg gcggcggtgg caactgcggc tttggtcttc attacgcgat
     3661 atccttgcgg gctagaggac gttaccctaa atcaacccgc gccgacttgg aagtgcaatg
     3721 aatattgaat tttccgccaa tgaggcccgc gtgatcggct gcctgatcga aaaggaaatc
     3781 accactccgg accagtatcc gctgtcgctc aatgcgctca ccaatgcctg caatcagaaa
     3841 accaaccgcg agccggtgct ggagttgagc gaggccaccg ttcagcaggc ggtggactct
     3901 ttgatgaaga aatacatggt gagcgacaag tccgccggtt acggcggccg ggtcaccaag
     3961 tacaaacacc gtttttgcaa tacggaattc ggctcgctca agttctccaa acaggaactc
     4021 ggcatcctct gcgtactgct gctgcgcggt ccgcaaactc cgggcgagct gcgcgcacgc
     4081 accaaccggc tgtgcgagtt caccgacgcg gaccaagtcg aagccacctt gcgcaacctc
     4141 atgacgcgcg aagatggtcc ctttgtggcg cgattgccgc gggctgccgg tgcgcgcgag
     4201 tcgcgctacg cgcacctatt ctcgggaaac atcgagtcgg tggaggaaac cgcgcccctc
     4261 gaggagccca gcgtcgccgg cgcccccacc ttgagtcaac gcgtcgccga cctcgaagaa
     4321 tcggtgcggc ggctgcgtct ggagttggac gcgctgagcg cagccaagca gggcgcgact
     4381 actgggggtt agccgccgtt tgcttggcaa tcgtctcgcg ctcgtcgagc cacttgccga
     4441 aggcctcgat gtccttgggg cgcatgccat tcgccgcgag acggtgactc tccagccact
     4501 tctgcatcgt gaccagcaac tgatccgcga gcgggcgcga gtggttgaac aggctcatgt
     4561 atgccgcctg agcgtcgtcc aatcggtcca cggccatatg cgcctcggcg cgatgttcga
     4621 ccgcctcgag cagattcgga tcgagcgcga gcgtgtgatt gtagtcgtcg attgcctcgc
     4681 cgtacgcgcc gagctgcaaa tgaatgtatc ccacgttgtt ccaggcttcg accatatcgc
     4741 ccttgttgct caatgcctcg gtgaattgat cgagcgccct accgtaagcg tcgctgacct
     4801 tgtccaacgc atcggctttc ttgtcgggat tggtcgcctt ggcggcgata tccgtatact
     4861 cgcgcgcccg attcagagat ttgacgccgg cttgaaaggc cttcttcgct gctttgtcgg
     4921 gcttttcggg ggccggcgta ttgggtatct catcgcccgg agcggactgc attcctcccc
     4981 ggccgcccgg catcccgccc ccctgcatcc cgccgcccgg atatccctgc ccgcaggcga
     5041 gctgcgggcc ggcggcacac catgccaaca gcagcatgca actgatgact atccgacgca
     5101 ttgtgctccc cgtctgcggc ttcgcccgtt tgtgcaacac tcgatccatg catggcaatg
     5161 gtaagttttt agcgctgcgt gcgctggcct ttttgtcgat gcacggtccg agggcctccc
     5221 ggtcgcccgc tcgaccgcgc tgcatcgcac ttggcgtgcc gaccgcgctg cgcgagacct
     5281 gcgccgcggc cgctcattcg ccatgacgcg aagccgcctc gatgccgatc cgcagtgtgt
     5341 tggtcgcgat acgtacttcg atgaggaacg ccgtcaaggc tcccgccagc gtcaacatcg
     5401 acaggatgaa catgctcgcg atgaacaccg ataagtccca acgcagaaag gcgttgacga
     5461 atagcatgac cacgaccaat gcaatgaaca gggcggacag ggtgatcagg gtgatcgccg
     5521 agttgatata gcgggcgcgg cgcgccagca cgcgcagcgt ttcgtgcagg tcgcgcgcct
     5581 cgtgcgtacg ctcggactcg cgcacttcat cttcgatcgc ccgcgcacgg tcgacgatgc
     5641 gcgccagacg gttggtcaag acgttgagcg tgatgccgat ggccgagatc aggaaaatgg
     5701 gggtcagcgc aatttggatg agatgcgcga tgtcgacggc attcattcgc gggcacccac
     5761 ctgaacctga atgcgcaacg tgccggcgtc gcgaaattgc aaattgagcg gaaactgcat
     5821 gcccgccgcc aacggcgtgc tcaagccgag caacatgacg tgcacgccgc ccggctcgat
     5881 tttggtaaca cccggcggac actcgatcca ggcgacggcg cgcatctgaa tcgtcccctg
     5941 caccgtacgg ctttcgtgta tttccacctg gcgggcgatc ggagtggaga tcgccagcaa
     6001 gcggtcggtg tcgcgcccgc tgttggtaat cgaaaaatag gcggcgccga ccgtggcgac
     6061 cgacggggtt ggcggcgacc atgccccgcc aaccgtcaac gcccccgcac gaaattccgc
     6121 tgccggcgcg ggtgccagga aaagccacag agccagcaac gccgcaccgc acactcgctg
     6181 gttcttgtga cgcacggcgt taagtatacc catctcgcct tcgcggctgc atccagatcg
     6241 cttggcgcca cgtacagctc ctacaagcgt ggcattcgga ccgcgcgcac cggacttaag
     6301 gaggattttc atgtctaagt acccctggac cgtggccgcc gcgctgtcgc taccgatgct
     6361 cgcattcgct tccagccatc gcgaggcgcc gaacatctcc cgcctgccga ccctggatgg
     6421 tactgatttc tacatgttca acagctacga aagcggccgc gccggctacg taactttgat
     6481 cgcgaactac attcctctgc aggaccccta cggcggcccg aactatttcg cgctcgaccc
     6541 ggccggtttg tacgaaatag agatcgacaa caatggcgat gccaaggaag acctgacctt
     6601 ccagttccaa ttcaccaaca cgctcgcaaa caagaacgcg ggcatcacgc ttcccgtggg
     6661 tcccgccgca agtccagcga cggtcgcggt gccgctgaag aatgtcggtc cgatcagcgc
     6721 gtcgagttcg gcgaatctta atttcatcga aacctacacg gtgacgctgg tcacgggcga
     6781 tcgccgcagc ggcgtcgcga cacccgttac caatgccgcc aacggctccg ccacgttcgt
     6841 caagccgatg gattacatcg gacaaaagtc gttcggctcg gacgcgggct acgccgccta
     6901 tgcggcgcag tacatgtaca ccatcaacgt gccgggctgc agctccccgg gccgagtgtt
     6961 cgtcggccag cgcaaggaag gcttcgcggt caacctcggc gaagtattcg acctcgtgaa
     7021 tttcgtgccg atcgaaggcg acagttcccc gggcgccggc gacggcaaag gctttcccgg
     7081 cgggatcacg caaaacccca gccacaacgt tctcgccaac aagaacatca ccagcatcgc
     7141 gctggaacta cccgccgcgt gcctcaaagg gtcgggcaat ggcgtgatcg gcggttggac
     7201 caccgccagc ttgcggcagg ctcgaattct tgacccgaac gcgacatttt cgaaacctga
     7261 agtgaacggc ggcgcctgga cgcaggtgtc gcgcctgggc atgccactgg tgaacgaagt
     7321 cgtaatcggc cttccggaca aggatcattt caactcgagt tcgcccatca acgatgcgca
     7381 gtttgcgacc tacgtgacca atccgagcct gcctgcgctg ctcaatgcgt tgtttctagc
     7441 gccggtcaat gccaccctcg ggacctcgat cgcaaacctc gcgccgaata attttccgcg
     7501 caacgatttg gtcgcagcct tcctcacggg atttgccggg ctgaaccagc aagcgaccgt
     7561 gactgcatcg gaaatgttgc gattgaacac cgcggtggcg gcaacgcccg cgacggcgca
     7621 gagcttcttc ggcgtcgcgg gcggcgacct cgccgggttt ccaaatggcc gacgccccgg
     7681 cgacaatgtc gtggatatcg cgctgcgcgt cgtgatggga gcgctctgct acccgctgcc
     7741 gatcggggcc aacaaggcgc ccaccaattt aggactgtgc acgcccgcaa atgcgcctgt
     7801 cggcaatgta cccttcaccg acggcgcgcc cgtcaatgcg ctcgacttcg atcaaacctt
     7861 cccctatttg aagacgccgc tgccgggctc acccatcggc tcgatgtaag gaaagcaacc
     7921 catgttctac acttcagtaa gatggcgttt ggcggcaggc gcaatgctcg cggcggtggt
     7981 cgggtgcagt ggcggcggcg gcggcagccc ggcgatgacg accccgacgc ctcccgccac
     8041 tcagcaatca accgattttt cctcgctcgt ctacatgatc tatgggcagc ccgcgaacag
     8101 ccagcccgtc agcttggaca atttgaccat cgtctacgat gtcaacgatg accccaccga
     8161 cttcaatggt ctgttgatgt aggtgcgcga atccggtcgc tacgagcggc gaggttcaga
     8221 agccgctttt gccaacggcg aacaccacga ctgcagcgaa taggcagtag tagccgaaca
     8281 gatgccagcg gccgtgctcg agccatcgag acagccactt gagagacagc catccggcaa
     8341 tgaagctcaa ggccacgccc atgacgctcg gccaaatcag acccgtgctg ccgctcgtga
     8401 ggccggcggc ggcatgcgcg ccgagcagcc gatacccttc gcgcgcgatc accggcggcg
     8461 tgagaatgac cgcgagtgca aaactgtaat tctccagcaa ttccttgtcg agaccgcgca
     8521 acaatccggt ggagatggtc gcccccgaac gcgagaagcc tctgaatggc aggcagagcc
     8581 cctgtaccat gccaatccat acggcatccc tgaactcgac cgactgcctg gctttggccg
     8641 ccgaacgcag tccggacaca atgatcaaca cgccaaccgt cgcaagcgcc acggcaatca
     8701 gagtgagatt gccgaacagc agttccacct ccgccttgac cgcaccgcga aggatgacgt
     8761 gctcgatgaa aacgatcagc ccaaagccga tgatcgccgt gcacactgtg gcgagaatca
     8821 aactgcgcgc gaacgaccag aaggcgctcg cagaggagaa gtaacgactg cgccaggtat
     8881 tccaaaaata ggcgatcacc gccagcatcg tgccggtgtg caacatgacc aataaaaaag
     8941 tcatagcggg tgtcgtcgga tccaagccca tcagcttttc agcgatgatg acatgcgcgg
     9001 aactcgacac gggcagcagc tcgcagaacc cctgcacgat cgcgagaatg actattttta
     9061 tgacaaacgg cacaacggcc ccttaataga aaacgccccg accgccgtga ggcggtcggg
     9121 gcgttatatc gattatctta cttggttgtc gcaggagcag gagctttctt gtgcttcttg
     9181 tgtgacttct tcgcgggctt cggcgtttcc gtggcagtgc cggcggcagg cgtatcagca
     9241 gccatcggcg cggcaacctt cggcatatcg gcagacatcg cggcagccgc gaggccaaga
     9301 gtcaacaatg cagtggtaat cagggttccg gacttattca tgagatctcc aagcggtggt
     9361 attgacgagc atgtcgaaga atacctcgga tccggttgtt tcatcacaga tgaagatcat
     9421 ttcacaaagc gccaggcggc gaatcggcgt gtttgccccg ggtccgaaca tgccacgcga
     9481 gatagatgac gatggcgatg ttggcgatca gcaccaccgc cttgccgaga ttcggccgga
     9541 aaagcagctc gtagacttcc aacggtatca acgaggcagt gatgatcgtg gtcagccatt
     9601 cagcccaacg tttctgcatc cataatccga cgccctccgt ggcgaatagc gcagcgtatg
     9661 ccaacgtcac gatgcgcagc gcgtgaacct gcgatgcact caagccgctg aacttctcca
     9721 acagccacgt caccacgtcg tgctccaatc ccaaggggcg agccgaagcc caagtcaaca
     9781 gtttggcggt gagcgaagca tcgctcaggc gcacctcgcc gtaggcgatc gcaagcagca
     9841 gcagcacctt caccagcttg taaagcgcga tggtacgaag gacgttgagg cgcggcagat
     9901 gcgccggtcg atcagcggtt atcatggaac gttaggcctg acccgccggc ggctgaaatt
     9961 ccaaggtggc atcgagcagc gccggaatgt cgaaaaccgc ccgctcgttg tcaagcacca
    10021 agggagcgat tccggcggcc gccaccagcg ccgcgggcac ttgaatgcgg tcgcgctgcc
    10081 gggtcaccgt ccaagtctcg agccgtttgt cttgaaacgc caaattgagc gccccccagc
    10141 gcaaaagggc ggctcgcgcc agcggtgtat ccccgcccca gtgacgctgc gccaatgcca
    10201 aggccgaact tgcggcgata tccccgtgcg gcagcgtcat caaatcctga atgtatttca
    10261 ccatttcgtc cggcggaacg tgcaagtgca agtaagatgc cgtctcggtc atggccgcca
    10321 ttttatgtcg gattctccaa gctgaacatg gcattctcgt catcgtacgc gaatgcctcg
    10381 gcatagcggg cccagcgcag cacggcgcgc agggtttggt cggccgcctc ctcgctcatg
    10441 aagtcctcca gctcatcgat gaatcggctc ttgcgcgccg aatgcgaggc gcgttcatcc
    10501 aaaacccggc ggatatgcgc ggccaggggc acatatgtca acaaatgacg cgagaaaatc
    10561 cgcttgcgtt catcacgcgc ggcttgcgcg aaagccagcc catcgggcgt cagtttcaaa
    10621 tccccgccct ccagctcggc gaagcgcaac atttgcaggg tttccgcgac cgggaacaat
    10681 tcgtcgatct ccatctgcaa atccgaggcg attttcggca agtctgcggt accgttgaag
    10741 ggcgccgccg cgaccgcctc catcaggccc gccagaagat tcgaagacac ctgggggtat
    10801 tggctaccga taccgaggcc gggaaaacgc tcgtgacgcg cgctgccggc tgcttgcggc
    10861 cgtgccgtca tctccacata gatccgctcc accagctcgc gaaagctcgg gtcgagccga
    10921 ttgcgcggct gcggcagtga gaccttgatt tcacttaaaa ttctgcccgg attgctgccg
    10981 aataccaaaa tgcgatcgca catcaagacg gcttcttcga tgttatgcgt cactaaaatc
    11041 acgcccttga tcggcatgcg gccttccgac cacaggtcca gaaaatccgt gcgcagcgtc
    11101 tccgcagtca gcacgtcgag tgccgagaat ggctcgtcca tgagcagaat attcggatgc
    11161 accacgagcg cgcgggcaaa tcccacgcgt tgacgcatgc cgcccgaaag ctcgcgcgga
    11221 taggcggatt cgaatccgtc gagcccgatg aggtcgatgg ccgccagtga tcgtttgcgg
    11281 atgtccgcgc gcgccatgcg ctgcgcctcg aggccgagtg caacgttttc gaacaccgtg
    11341 agccagggaa acagcgcgaa gctctggaac accatggcga tgctggatgc gggtccgacg
    11401 atcggctgcc cgaggtagca cacctgaccg ccggtcggct ccgccaatcc cgcgatcaag
    11461 cgcaacaaag tcgacttgcc cgagcccgac ctacccagca acccgacgat ttgcccctct
    11521 cgcaattcga gattcatgcc ttcgaggacc agcaattccg caccgtccgg cttgggaaat
    11581 gccttgcgaa cctccgtgca ctgcagtagc ggcgagctgt tcatgaccaa aacctcaggt
    11641 aagccgatat ttgcgctcgg cgtagtaata caaggggcgc cagaagagcc gattgatgat
    11701 gacgacgaac agactcatgg tgcctatccc caaaacgatg cggtgaaaat ccccggcagt
    11761 cgtggcttcc gcgatatatg aaccgaggcc ccgcgcctcg acttgagttt tcccccacgt
    11821 ggccaattcc gcgacgatcg cggcattcca cgatccgccc gatgccgtga tggcaccggt
    11881 gacgtagaac ggcagaattc ccggcagcgc gaccttgcgc caccacagcc aacccctgac
    11941 tcggaaattt gctgcaacgt agcgcatttc ggccggtatc gccgacgcgc ccgcgatgac
    12001 gttgaacaaa atgtaccact gagtgcccaa tatcatgagc ggactcaaat aaatatccgg
    12061 gttgagcttc cacgcgacga tcccgtagac cgcgagagga aacaacagat tcgccgggaa
    12121 tgccgccatg aattgcgcca ccggctgaac tatctgtgca acctgcggcc gcatccccac
    12181 ccacacaccg atcggcaccc acaccaagct cgccagggcg atcaatatga agacgcgcaa
    12241 catcgtcaag gtcgcgagac cgcatacctg caacgcctcg tgcagcggcg tattcttgat
    12301 cagcaccagg acgatgtgcc agcagccgag cagtacggcg gcggcgacca cggtcaccca
    12361 caagaaatcc aaactgcgct tgcgaaccat ggggttctcg acgctcggtt tatcgctgcg
    12421 cctgacggcc cggctggtcc acagcaccgc cgcatgaaac gccaaggatg cggccagaat
    12481 gacgcgcgaa cggcgcatca tcgtcagcgc ccacgagtca ggaacccgca caccgggctc
    12541 ctgctcgacg cgaaaccggt ccacccacgc caccagcggt cgaaacaaca gctggtcgta
    12601 cagcaggatc acgatcaaca tggtggcgat tgcccagccg atggcatgca ggtttttctg
    12661 ttcaatggcg agcgcgatgt aggaaccgac accgggcagc gcgacggtgg tatgtccgac
    12721 gctgatggat tcggaggcca cgacgaagaa ccagccgccc gacatggaca tcatcatgtt
    12781 ccatatcagc gccggcatgc cgaagggaac ctccaactgc caaaagcgca tccacggcga
    12841 gagccgaaac gactccgccg cttcggcgag ttcggtcgga atcgtgcgca acgactgata
    12901 gaagctgaaa gccatgttcc aagcttggct ggtgaatatc gcaaagatcg ccgcgaactc
    12961 ggcgccgagc acgcggccgg gagccagcga catgaaaaac acgaccgtaa tcgaaatgaa
    13021 acctagaatt ggcaccgact gcagaatatc cagtatcggc accaggagct tccccgcccg
    13081 ctcgctcttc gccgcccacg tcgcgtaagt cagggtgaag accagcgaca ggaacagacc
    13141 cgccaacatg cggaacgtgg tgcgcgcggc atattcgggc aaatggctgg ggctgagcga
    13201 caatggcgtc atttcgagcg tcgacagcgg cgagaacaag ccgcgcgatg tttcgcccaa
    13261 aaaggcgatc aagccgatca cgacgatgaa tgccagcacg tcccaacgcg accagaagtc
    13321 gcgcagaaag acatagggag caaagcgatt catccgcgca ttctctcagg acagacaacg
    13381 aagtagctag tagggtacgc atttatggac aagagtttca caggcgattc gcgggctatc
    13441 ggcacactgc gagccgcggc gccgctgcaa agccaggcaa tgccgacgcg cttcaatgtt
    13501 ctgggcgcga tcagcttctc gcactttctg aacgacatga tgcagtcgct gatcgttgca
    13561 atctatccgt tgttgaaggg cgaattcagc ttgagtttcg tgcagatcgg cctcatcacg
    13621 ctgacctatc aaatctgcgc ctcggttttg cagccgctca tcggcatcta caccgacaaa
    13681 cacccgaagc cctactcgct gagcgtcggc atgggattca cgctcattgg catggtggct
    13741 ttggcgtttg cgccgaatta cgccttggtg ttggtcgccg ctgcgatgat cggtgcggga
    13801 tcgtctattt ttcatcccga gtcctcgcgc atcgcgcgcc tcgcctcggg cggccgtcat
    13861 gggctggcgc agtccatttt ccaggtcggc ggcaatgcgg gcagcgccat gggtccgctg
    13921 ctggccgcat ggattatcgt tccgcgcgga caatcgagtc tcgcctggtt tgcgctggct
    13981 gcgttgctcg ccattgccgt gttggcgcgg gtcggcgcct ggtacaagcg ccagcatctc
    14041 gagcgagccg gcgccggcag gcgagcagcc gtgccgagtc cggtgtcggc gcgccgggtc
    14101 gcatggtcga tcgtcatcct ggttgcattg atattctcaa agtatttcta tatcgcgagc
    14161 atctcgagct tctacagctt ctacctgatc gaaaaatttc acctgtcggt tcgctcggcc
    14221 cagatgtacc tgttcgtttt tctgcttgcc atggcgctcg gtacgctgtt cggcggcccg
    14281 atcggcgatc gcatcggccg caagcgcgtc atctgggcat cgattctcgg agtagcaccc
    14341 tttagcctgc tgctgccctt cgttggtttg atgtggaccg gcatcctcac cttcatcatc
    14401 ggactcattt tgtcctcggc gttctcggcc atcgtcgtat tcgcccagga gctgatgccc
    14461 ggaaaggtgg gtgccgtttc gggattgttt ttcggattcg cattcggcat cggcggcatc
    14521 ggcgcggcgg tgctcggcgc tgttgcggac agccacggca tcgaattcgt ctaccggctg
    14581 tgcgcctacc tgccgctgct cggcatggtg gcggcgttct tgcccgatat cgaacatcac
    14641 aggacatgac gcaccaaatc atacttacga tcggtcaata tgggttgatg atcgtgttcg
    14701 tcaacgtgct gctcgatcag attggacttc ccgtgcccgc ggtgccgacg ttgatcgtcg
    14761 ccggcgcaat cgccgcagat ggacagctgc cgttggcctc gctcttcgtc tggtccgtga
    14821 tcgcctgcct ggccgcggac tgcggctggt acctgatcgg tgagaggtac ggcatccgcg
    14881 tcctcaagac gctttgcaga atttcgctcg aacccgattc gtgcgtcagc cagacccaaa
    14941 cccggttcga acgctgggga atcaatt
//
LOCUS       DASWZT010000364         3904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_12684, whole genome shotgun sequence.
ACCESSION   DASWZT010000364 DASWZT010000000
VERSION     DASWZT010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12684"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VGE92_10295"
     CDS             <1..346
                     /locus_tag="VGE92_10295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HEY1314259.1"
                     /translation="EDDLFSTEVFEASQRSRRVDLDVGESMNGAEVPTNKLQSLESTA
                     RTAKIKSTDMALPELEPVTMSEVGTKLDLARAYMDMGDPEGARSILEEVVQEGSASQK
                     QEAQRLIETLPG"
     gene            362..1162
                     /gene="truA"
                     /locus_tag="VGE92_10300"
     CDS             362..1162
                     /gene="truA"
                     /locus_tag="VGE92_10300"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEY1314260.1"
                     /translation="MEQGHRVAAIVEYDGTDYAGWQSQEHSASIQDLVQAALSFVAGE
                     PVAAICAGRTDSGVHAIGQVIHFDTHAARTARAWVLGANTKLPPAIALQWAGEVSSGF
                     HARHKAIRRIYRYCILNRSERSGLQRARAAWIHRPLDVAAMHAAAQALVGEHDFSAFR
                     SIQCQSKTTLRRVERIDVRREGDYIWFEIAANAYLHHMVRNIVGTLIAVQRDSDAACA
                     MARVLAGSDRRFAGATAPAAGLYLWRVEYPPIYAIPTVDSGRMLPSSG"
     gene            1179..2042
                     /gene="accD"
                     /locus_tag="VGE92_10305"
     CDS             1179..2042
                     /gene="accD"
                     /locus_tag="VGE92_10305"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEY1314261.1"
                     /translation="MSWFERIVPSRIKTERRTRSVPEGLWTKCPACDAVLYRAEIERN
                     LYVCPKCSHHMRINGRERLAKFLDPGATTELAARVEPEDPLKFKDSKRYKDRLTQSQK
                     QTGERDALIVMAGKLAGLDLVACAFEFRFLGGSMGSVVGEKFVVAVEHCLEHRMPLVC
                     FAASGGARMQEALYSLLQMAKTSAALKRLSEARLPFISVMTDPTTGGVSASLAMLGDI
                     NIGEPKALIGFAGPRVIQQTVRETLPEGFQRSEFLLEHGALDMIVDRRELRDRIGSML
                     RLLTGKEPAAA"
     gene            2056..3357
                     /gene="folC"
                     /locus_tag="VGE92_10310"
     CDS             2056..3357
                     /gene="folC"
                     /locus_tag="VGE92_10310"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF008101.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEY1314262.1"
                     /translation="MSSLEQWLAYQTQVHPQAIDLGLDRLREVLGRLGWRQPDIPVVT
                     VAGTNGKGSVSGYCAAILTAAGYRVGTFTSPHLRDYRERIQVHDRLVSAAELVAAFER
                     IEAARGAVGLTFFEFNTLAALLVFEASRLDAWILEIGMGGRLDAVNVVDPSVAVVVSI
                     GFDHQEYLGSTLEAIAREKGGILRHGRPAVLGSSDMPPVLEEMARAIGAPLKRPGIEY
                     SYAVDGHGWQFRGAHWNLPKLPAPALLGVAQYANAATALAALEECDARLPVPAAAVAR
                     GLREVRMPARFEVLAPRAHQPTWILDVAHNPDAAKVLARNLHELTSGGLTLAVCGILA
                     DKDASGVAAALREHIDAWWCVTTDGERGRSGDSLARTVALEVAAPVLAAASAAAGCAA
                     ALAAATAADRIVVFGSFHVVGPALDWLDAHDYTAAPRPTYV"
     gene            3367..>3904
                     /locus_tag="VGE92_10315"
     CDS             3367..>3904
                     /locus_tag="VGE92_10315"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY1314263.1"
                     /translation="MDRRVKERLVGASILVVLIVLIVPELLSGPAPVPVPAPAPMLSA
                     APASAAEPVRNVTVDLATSKAPAPEPPPEPAAASSPPHEPASAAAASAVEAPTRLETA
                     PPAPSSAAAPTKPAALPGRSWAVQLGSFASRANADKLVRQLKAQGFAAYVLPGGSGTS
                     LRYRVRVGPMADRNSAAQA"
BASE COUNT          668 a         1227 c         1320 g          689 t
ORIGIN      
        1 ggaggacgac ttgttctcga ccgaagtatt cgaggccagc cagcgcagcc gccgcgtcga
       61 tttggacgtg ggcgagtcga tgaacggcgc agaggtaccg accaacaagc tgcagtcgct
      121 cgaatcgacc gcgcgcaccg cgaagatcaa atcgaccgat atggcactac cggaattgga
      181 gccggtgacg atgagcgaag tgggtacgaa gctcgatctt gcacgggcgt atatggacat
      241 gggagaccca gagggcgcgc gcagcattct cgaggaagtt gttcaggaag gatccgccag
      301 tcagaaacag gaagcgcagc gcctgatcga gaccctgccc ggctaagatc cgagccggag
      361 catggagcag ggccatcgcg tcgccgccat cgttgagtat gacggcacgg actacgccgg
      421 ctggcagtcg caggaacact cggcgtcgat ccaagatctc gtgcaggctg cgctgtcgtt
      481 cgtcgcgggc gagccggtcg cggcgatttg cgcgggccgt accgatagcg gcgtgcatgc
      541 gatcggacag gtgattcact tcgacaccca tgccgcgcgc accgcgcgcg cctgggtgtt
      601 gggcgccaat accaagctgc cgccggcgat cgcgctgcaa tgggccggcg aagtgtcgag
      661 cggctttcac gcccggcaca aagcgatacg ccgcatctat cgctactgca tattgaaccg
      721 cagcgagcgc tccggcttgc agcgtgcgcg tgcggcttgg atccatcgcc cgctcgatgt
      781 ggcggcgatg cacgctgcgg cgcaggcttt ggtcggcgag cacgatttct ccgcctttcg
      841 ctcgatacag tgccagtcta aaaccacgct gcggcgcgtc gagcgcatcg atgtgcgtcg
      901 cgagggagac tacatttggt tcgagattgc agcgaacgcg tacctgcacc acatggtccg
      961 taacatcgtc ggaacgctca tcgccgtgca gcgcgacagc gacgcggcgt gcgccatggc
     1021 gcgggtgctt gccggcagcg accgccgttt cgccggcgcc acggcgcccg cggcgggcct
     1081 gtatttatgg cgcgtcgagt atccgccaat ctacgccatt ccgacggtgg actccgggcg
     1141 catgctgccg tcgagcggct gatgggctaa actagcgtat gtcctggttc gagcgaattg
     1201 tcccttcacg gatcaaaacc gagcgccgca cgcgctcggt gccggaaggc ctgtggacca
     1261 agtgtccggc ctgcgatgcg gttctttatc gcgccgaaat cgaacgtaat ctgtacgtgt
     1321 gcccaaagtg cagccaccac atgcgcatca atggccgcga gcggctggct aaatttctcg
     1381 accccggcgc caccacggaa ctggcggccc gggtcgagcc ggaagatccg ctgaaattca
     1441 aggattcgaa gcgctacaag gaccggctca cccaatcgca gaagcaaacc ggcgagcgcg
     1501 atgcgctgat cgtgatggcg ggaaaattgg ccggcttgga tctggtcgca tgtgcgttcg
     1561 aattccgttt tttgggcggt tcgatgggct cggtggtcgg tgagaaattc gtcgtggcgg
     1621 tcgagcactg cctcgagcat cgcatgccct tggtgtgttt tgccgcaagc ggcggcgcgc
     1681 gcatgcagga ggcgctgtac tcgctactgc agatggcgaa gaccagtgcc gcgctcaagc
     1741 gcctgtcgga ggcgcgcctg cccttcattt cggtgatgac ggatccgacg accggcggcg
     1801 tgtccgcgag ccttgcgatg ctcggtgaca tcaatatcgg cgagcccaag gcgttgatcg
     1861 gttttgcagg tccgcgcgtg atccaacaaa cggtgcgcga aactttgccc gaagggttcc
     1921 aacgcagcga atttctgctc gaacacggcg cactcgacat gatcgtcgat cggcgtgaat
     1981 tgcgcgatcg catcggatcg atgctgcgtt tgctgaccgg caaagaacct gccgcagcct
     2041 aaagccggcg gcgccatgtc gagtctcgag cagtggctcg cctaccaaac gcaggtgcac
     2101 ccgcaggcca tcgatcttgg cctggatcgc ctgcgcgagg tgctcgggcg gctcgggtgg
     2161 cggcagcccg acatcccagt cgtcaccgtc gccggcacca acggcaaggg ctcggtgagc
     2221 ggttattgcg cggcgatcct gacggcggcc ggttatcggg tgggcacctt cacttcgccg
     2281 cacctgcgcg attaccgcga gcggattcag gtccatgacc gcctggtgag cgctgcggaa
     2341 ctggtcgcgg cgttcgaacg gatcgaagcc gcgcgcggcg cagtgggtct gacttttttc
     2401 gaattcaata ccctcgccgc gctgttggta ttcgaagcgt cgcggctcga tgcgtggatt
     2461 ctcgagatcg gcatgggtgg ccgtctggac gccgtcaatg tagtcgatcc cagcgtcgcg
     2521 gtggtcgtga gcatcggatt cgaccaccag gaatacttgg gtagcacgct cgaggccatc
     2581 gcccgggaaa agggcgggat cttgcggcac ggcaggcctg cagttctcgg cagcagcgac
     2641 atgccgccgg tgctcgagga aatggcgcgc gcgatcggcg cgccgctcaa acgcccagga
     2701 atcgaataca gctacgccgt cgatggccac ggctggcaat ttcgcggcgc gcactggaat
     2761 ctgccgaagt tacccgcgcc ggcactgctt ggagtcgcgc aatacgcaaa tgcggcgacc
     2821 gcccttgcgg ctctcgagga gtgcgacgcg cgtttgcccg ttcccgctgc ggcggtggca
     2881 cgcgggctgc gtgaggtgcg catgcccgcg cgcttcgaag tgctcgcgcc gcgcgcgcac
     2941 cagccgacat ggatattgga cgtggcgcac aatccggacg ccgcaaaagt cctggcgcgc
     3001 aatctgcacg agcttacaag cggcggactg acactcgcgg tgtgtggaat cttggccgat
     3061 aaggatgctt cgggggtggc cgcggcattg cgcgagcaca tcgatgcgtg gtggtgcgtt
     3121 accacggatg gcgaacgcgg ccgcagcggc gattccttgg cacgcacggt cgcgctcgag
     3181 gtcgcggcgc cggtccttgc agcggctagc gccgccgccg gatgcgcggc ggctctcgcg
     3241 gcggcaaccg cggcggatcg gattgtcgtt ttcggttctt tccatgtggt agggccggca
     3301 ctcgattggc tcgacgctca cgactatact gcggcgcccc gacctactta cgtttgaggc
     3361 tgtgttatgg atcgtcgcgt caaagaacgc ctggtcggtg cctcaatctt ggtggtgctc
     3421 atcgttttga tcgtgcccga attgctgtcc ggccccgcac ccgtgcccgt gccggcaccc
     3481 gcgcccatgc tgagtgccgc accggcgagc gcagcggagc cggttcgcaa tgtcaccgtg
     3541 gatctcgcga ccagcaaagc gccggcgcct gagcccccgc ctgagcctgc cgcggcgtcg
     3601 agcccgccgc acgagccagc gagcgcggcc gccgcgagcg ctgtggaggc acccacgagg
     3661 cttgaaaccg ccccgccggc ccccagttcc gctgcagcgc cgacgaaacc ggcggcgctg
     3721 cccggacgct cgtgggccgt gcagctcgga agtttcgcga gccgcgccaa cgccgacaaa
     3781 ttggtgcggc aattaaaggc gcaaggcttc gccgcgtatg tattgccggg aggctcgggc
     3841 acctcgctca ggtaccgggt tcgtgtcggg cccatggcgg atcgcaactc ggcggctcag
     3901 gcgg
//
LOCUS       DASWZT010000365         2084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_60948, whole genome shotgun sequence.
ACCESSION   DASWZT010000365 DASWZT010000000
VERSION     DASWZT010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60948"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..1908
                     /locus_tag="VGE92_10320"
     CDS             73..1908
                     /locus_tag="VGE92_10320"
                     /inference="COORDINATES: protein motif:HMM:NF017078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HEY1314264.1"
                     /translation="MSIRTKMISLLALLFAILIVIEIAVQQQVLMPSFAQLEREDAET
                     SMKRIGYALDMALEGLEVTTADWGNWADVYQFVQSPDTAFVTANITAVAMKQLQVNAI
                     LIVDLDGRYVLSSARSLESGEPIDLNLAARTALPLDFPWRRNLLDGKPARGLVRTNHG
                     VMMIAAAPVLDGSGGGQPLGMVLMGRLLTPAQVQMIGAQAQARLTMLRDRLSGATEID
                     ETDAVTRVSKPFTDIYGKALMRLRVEVPRRITARGQGAVTYASLYLIGAAVTVLLLLV
                     VAVNRIVLRPLARVTRHAVGIGEGTDLSARLGIAGRDEVALLAREFDRMVERVAESRR
                     QLVDQSFQAGFAELAKGVLHNLGNAMTPLGVRVAKLSERLHDAPAADVDIALAELAKE
                     PEGSTRRADLQEFLRLACRELAASVKEAESDVAVVQRQATIVQSALSELMRSTRNPHV
                     VESVRLPELVAQTLEIVPDNCRQRLVVNADESLRRIGAVRVARTVLRLILQNLIINAA
                     DAVRDAGRDKGVLNFAAEIIADAESRQLHLHCKDDGVGIAADNLQRVFDKGFSTKSRE
                     TNYGIGLHWCANAIGALGGRIWAASEGPGLGASMHLMLPLPLAST"
BASE COUNT          388 a          587 c          722 g          387 t
ORIGIN      
        1 gcacggccct aatcccgatg aaataagaca cgagcacggc cgcagcacgg gaattctgct
       61 accaataagc tgatgagtat ccgtacgaaa atgatttcgt tactggcact gctgttcgcg
      121 atcttgatcg tcatcgagat cgcggtccaa cagcaggtgc tgatgccgag cttcgcgcaa
      181 ctcgagcgcg aagatgcgga gacgtcgatg aagcgcatcg gctatgcgct cgacatggct
      241 ctcgagggct tggaagtgac gacggctgac tggggcaatt gggcagatgt ttatcaattc
      301 gtacagtcac cggataccgc gttcgtgacc gccaatatca ccgcggtggc gatgaagcag
      361 ctgcaggtga acgcgatact catcgtcgat ctggacggcc gctacgttct gtcgagtgcg
      421 cgcagcctgg agagcggcga gccgatcgac ctcaatctgg cggcgcgcac ggcgctgccg
      481 ttggattttc catggcgcag gaatttgctc gacggtaagc cggcaagggg actcgtgcgc
      541 accaatcatg gcgtcatgat gattgccgcc gcaccggtgc tcgacggcag cggcggcggg
      601 caaccgctcg ggatggtact gatggggcgg ctgctcacgc cggcgcaagt gcaaatgatc
      661 ggcgcgcagg cgcaggcgcg gcttaccatg ttgcgcgatc gcttgtcggg cgcgaccgaa
      721 atcgacgaga ccgacgcggt gacgcgcgtg tccaagccgt tcacggatat ctacggcaag
      781 gcgctgatgc gtttacgcgt agaagtgccg cgccggatta ccgcccgcgg ccaaggggcc
      841 gtgacgtatg cgtcgctgta cttgatcggc gcggcggtca cggtgttgct gctgctggtg
      901 gtggctgtca accgcatcgt gctgcggccg ctggcgcgcg tgacgcggca tgcggtcgga
      961 atcggcgagg gtacggacct gtcggcccgg cttggcattg cgggtcggga tgaagtggcg
     1021 ctgctggcac gtgagttcga tcgcatggtc gagcgcgtcg cggagtcacg gcgtcagctg
     1081 gtggatcagt cgttccaggc cggctttgcc gaacttgcca agggcgttct gcacaatctc
     1141 ggcaacgcca tgacaccgct cggcgtgcgg gtggcgaagc tcagtgagcg gctgcatgac
     1201 gcgccggccg ccgacgtgga tatcgctctt gcggaactcg cgaaagaacc tgaaggaagc
     1261 acgcggcgcg cggatttgca ggagttcttg cgcctggcgt gccgtgaatt ggcggccagc
     1321 gtcaaggagg cagagagcga cgtcgcggtc gtgcagcgcc aggcgacaat cgtacaatcg
     1381 gcgctgagcg aactcatgcg ctcgactcgt aatccgcatg tggtcgagtc ggtgcggctc
     1441 cccgagctcg tcgctcagac gctggaaatc gttccggata actgccgcca gcgattggtg
     1501 gtcaatgccg acgagtcttt gcgcaggatc ggcgccgtgc gcgtggctcg cacggtgctg
     1561 cggctgattt tgcagaacct aatcatcaat gccgccgacg ccgtgcgcga cgcgggccgg
     1621 gacaaggggg tgctgaattt cgccgccgag atcatcgccg acgccgaaag ccggcagttg
     1681 cacttgcatt gcaaggacga cggcgtgggc atcgccgccg acaacctgca gcgggtgttc
     1741 gacaaggggt tctcgacgaa gtcgcgcgaa accaattatg ggatcggcct gcattggtgc
     1801 gccaatgcga tcggcgcact gggcggccgt atttgggcgg cgagtgaagg cccgggtttg
     1861 ggggcgtcga tgcatttgat gttgccgctg ccgcttgcaa gcacgtgagg ttgccaatgt
     1921 ccgacagaaa aaagaaatcg ccgattcgtg tgctcgtcgc cgacgatgag gcggacgtgc
     1981 gcgatgccta tcggcaaatc ttgacggaag ccgatatgag cggcgaaacg gtaatgttcc
     2041 atagtttgcg cgagcggcta ttcagcaaga atgcgccgca gccg
//
LOCUS       DASWZT010000366         2141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_2940, whole genome shotgun sequence.
ACCESSION   DASWZT010000366 DASWZT010000000
VERSION     DASWZT010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2940"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="VGE92_10325"
     CDS             complement(<1..1302)
                     /locus_tag="VGE92_10325"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEY1314265.1"
                     /translation="MRRIFCVLLMLLTVPTMANDLTLERIFAEPALAGPTPRGVKISP
                     NGRRVGLLRGSDTDHHQLDLWTYDIKTGRLEKRVDSLRLAAHEQLSDAERARRERERI
                     AEYRGIVDYDWAPDGRHVLFTLAGILYLYDLDAAADTALRRLTQTAHPLLDPQVSPRG
                     HYASFVRDQDLWVIDLRSGAERRLTSDGGAAIHNAEAEFIAQEELRQTSGYWWAPDDS
                     AIAYKRFDESKVPIARRTEIYADRTAMVEQRYPAAGEPNVSVKLGLLAPGGGATRWVD
                     LGADPDIYLTRADWTPDAHHLAFQRLSRDQKRLDLVLVDAATLNQVPLLAETSATWVN
                     LSDDLHFLKRQRGFIWSSERSGTNHLYLYDLAGRLLHPLTRGAWNVDGLLAVDESTGT
                     VYFDSNRDAVQDLQIYTARLDGRDAENPGRISRGDGWHAAQF"
     gene            complement(1316..>2141)
                     /locus_tag="VGE92_10330"
     CDS             complement(1316..>2141)
                     /locus_tag="VGE92_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314266.1"
                     /translation="DGAMDQFHSDDFLLYAYLQTAQDARAKATIGASAAALTQFESMP
                     AMGDDYMVGMFPYYRTKLPVFFALEMRDWTSAAALETVPGATPETKTLTYWARCVGAG
                     HLRQAPQAKADLAEYDSLMQEVRRGRYAYYADSITSQVQRGEMLAWIAFAAGNREEAL
                     QHMRDAADLQDKVGQGEVDIPAREMRADMLLEFGAAREGAAREALAEYQSALKMSPNR
                     FNGLFNAGRAAEAAGDKLQAETYYTAVLKATDNGLQSSRPEFAHMKSFLAAALAAN"
BASE COUNT          387 a          682 c          683 g          389 t
ORIGIN      
        1 gaattgcgcc gcgtgccagc cgtcgccgcg gctgatgcgg cctggatttt cggcgtcccg
       61 cccatcgagc cgggccgtat agatctgcaa atcctggacg gcgtcgcggt tcgaatcgaa
      121 atagacggtg ccggtggatt catcgaccgc cagcagtccg tcgacattcc aggcaccgcg
      181 ggttagcggg tggagcaagc ggccggcgag atcgtaaaga tacaaatgat tcgtgccgct
      241 gcgctcggaa gaccagataa agcctcgctg gcgcttcaaa aaatgcagat cgtccgacag
      301 attgacccag gttgccgagg tctctgcgag caagggcact tggttgagcg tcgcggcgtc
      361 gaccaacacc aggtcaaggc gtttttggtc gcgcgacagg cgttgaaagg cgaggtggtg
      421 agcgtccgga gtccagtcgg cgcgggtcag gtaaatgtcc ggatcggcac ccagatcgac
      481 ccagcgcgta gcaccgccgc cgggcgcgag cagcccgagc ttgacgctca cattcggctc
      541 gccggcggcg ggatagcgct gctccaccat cgcggtgcgg tccgcataaa tctcggtgcg
      601 gcgcgcaatc ggcaccttgc tttcatcaaa gcgcttgtag gcgatggcgg agtcgtccgg
      661 cgcccaccaa tagccgctgg tctgccgcag ttcctcttgg gcaataaatt ccgcttcggc
      721 gttatgaatg gccgcgccgc cgtcgctggt caaacgccgc tccgcgccgc tgcgcaggtc
      781 gatcacccac aaatcttgat cgcggacgaa cgaggcgtag tggccgcgcg gcgacacctg
      841 cggatcgagc agaggatgag ccgtttgcgt cagccggcgc agcgccgtgt ccgccgccgc
      901 gtcaagatcg tagaggtaca gaatgccggc gagcgtgaac agcacatgcc gcccatccgg
      961 cgcccaatcg taatcgacga ttccgcgata ttccgcgatc cgctcacgtt cgcggcgcgc
     1021 gcgctcggca tccgagagct gttcgtgggc cgcgaggcgc agcgagtcca cgcgtttttc
     1081 gagtctgccg gtcttgatgt cataggtcca cagatcgagc tggtggtgat ccgtgtcgct
     1141 gccgcgcagc agccccacgc gacgcccatt gggcgaaatc ttgacgccgc gcggcgtcgg
     1201 gccggcgagc gcgggttcgg caaagatgcg ttcaagcgtg aggtcgttcg ccatggtggg
     1261 tacggtcagc agcatcagca gcacacaaaa aatacgacgc atcgatacgt gtcagtcaat
     1321 tcgccgcgag cgccgccgcc aggaacgact tcatgtgcgc aaactcagga cgcgaggatt
     1381 gcaacccatt gtcggtggct ttgagcacag cagtgtaata ggtctcggcc tgcagcttgt
     1441 cgcctgcggc ttcggcggcc cggccggcat tgaaaagtcc attgaagcga ttcggactca
     1501 ttttcagggc gctctgatac tcggccagcg cctcgcgggc cgcgccctcg cgggccgcgc
     1561 cgaactccaa gagcatatcc gcccgcattt cgcgcgccgg tatgtcgact tccccttggc
     1621 ccaccttatc ctgcaaatcc gccgcgtcgc gcatgtgttg caaggcctct tctcgattgc
     1681 cggccgcgaa ggcgatccac gccagcatct cgccgcgttg aacctgcgag gtgatggagt
     1741 ccgcatagta cgcgtagcga ccccgcctca cctcttgcat gagcgagtcg tactcggcga
     1801 gatcggcttt tgcttgcggc gcttggcgca aatgtccggc gcccacgcag cgcgcccagt
     1861 aggtgagggt cttggtctcc ggagtcgcgc ccggcaccgt ctcgagcgcg gccgccgagg
     1921 tccaatcccg catttcgagc gcgaagaata ccggcaactt ggtgcgatag taggggaaca
     1981 tccccaccat gtagtcatca cccatcgcag gcatcgactc gaactgcgtg agcgccgccg
     2041 ccgatgcgcc gatggtggcc ttggcgcgtg cgtcctgtgc agtctgtaaa tacgcgtaca
     2101 acagaaagtc atcggagtgg aactggtcca tggcaccgtc c
//
LOCUS       DASWZT010000367        12506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_2955, whole genome shotgun sequence.
ACCESSION   DASWZT010000367 DASWZT010000000
VERSION     DASWZT010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12506
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2955"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1819)
                     /locus_tag="VGE92_10335"
     CDS             complement(11..1819)
                     /locus_tag="VGE92_10335"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HEY1314267.1"
                     /translation="MSSTPVTSSADPGAKLAREAWIRALERTASIDQAALTLPALIDR
                     LAHQFDAAPALLSNDACLSYRELANRCNQYARWGIGQGLNAGDTVCLLMPNCAEYMAV
                     WLGLTRIGVVVALINTNLAGDALVHSINIVAPQAVIVGSELAARLTEVRARLRSALPC
                     WVYGNSAHDLAPLAPQLIRLSGAQLRDSECSPPRLDSSALYIYTSGTTGLPKAAKVSH
                     YRVMQWSHWFAGLLDTGPTDRMFNCLPLYHSVGGVVATGATLVGGGAVIIRARFSASD
                     FWRDVRDERCTLFQYIGELCRYLLNAAHQDLETSHSLRIACGNGLRPEVWEPFQNRFK
                     IPRILEYYASTEGNFSLYNCEGRVGAIGKIPSFLAHRLPVALLRFDVERGEPRRNDAG
                     FCERCLANEVGEAVGLIPAGGKELAGRFEGYADPEATARKVLHNVFKDGDSWYRTGDL
                     MRRDERGFYYFVDRVGETYRWKGENVSTAEVLTALTAARGVVDGVVYGVAVPGADGRA
                     GLAALLVNADFDLAAFRADVALKLPSYARPVFLRLLKNIESTGTFKPRKQDLLQAGFD
                     PARTSDPLYFDDSRSQAYVPLDAPLFAAISAGAVRV"
     gene            1915..3192
                     /locus_tag="VGE92_10340"
     CDS             1915..3192
                     /locus_tag="VGE92_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HEY1314268.1"
                     /translation="MKRETIAIHGGFDSDPATKAVAVPIYQTAAYSFDSAEHGAALFN
                     LEVPGYRYTRISNPTTDVLEQRVTELEGGVGSISVSSGQTALYYATANLTEMGRNIVS
                     VPQLYGTTYTLFAHVLPKMGVQVRFAKSDRAPDLEALIDADTRAIFCETVGNPAGNVC
                     DLEALADVAHRHRMPLIVDNTVATPMLVRPKEYGADIVVHSLTKFMGGHGTTLGGIVV
                     DCGTFSWTDNASRYPMFTQPDESYHGLVYTEHYGAAAFVGRCRSVYQRTLGAVLSPMN
                     AFLLLQGIETVALRVERHVENARKVAEFLRASPRVESVNFAGFEDNPYHPLVRKYLGG
                     KACSLMTFEIRGGLAAGMRFYNALKLFKRLVNLGDAKSLACHPASTTHRQMSPAEQLA
                     AGVAPGGIRLSVGIEHIDDILADLDQALRVAVG"
     gene            complement(3207..3485)
                     /locus_tag="VGE92_10345"
     CDS             complement(3207..3485)
                     /locus_tag="VGE92_10345"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEY1314269.1"
                     /translation="MDQKTIQGVGPQLVALVKQTLGATAVVPDPFPVDQQLSDLGISS
                     LKMVNLMLAVELEFDIAIPQSDITPENFHSVAAIQALVVRTLAQRKDA"
     gene            3746..4936
                     /locus_tag="VGE92_10350"
     CDS             3746..4936
                     /locus_tag="VGE92_10350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY1314270.1"
                     /translation="MSGAARVVNVRQNDLTARARAAADVAKANAAAVDAEARFPAESF
                     AEIRKQRLLGIQVPQSLGGEGASLAEIADVCYILGQSCSSTALIYAMHQIKMACMVRH
                     CKGNSAIERILSRIANEQLLMASSTTEGTAGGNVRSSEAAVVHEGAQVTLERKATVIS
                     YAVEADGVVTTARRAADAAGSDQVLLVLLKADYTLERLQVWDTLGMRGTHSEGFTLRA
                     RAAAEQIMPEPYDRIHAQTMVPFAHLLWGSVWAGIAAAAAGKAQAFIRMAVRNSSGQL
                     PPGAAHFTQAVSTLRTLRGVLSANLRSYEAIMGDDKAISSLEFQAMITLTKVQVSELA
                     VTTVLSSLRACGLSGYRNDTEFTIGRLLRDVLSAPLMINNDRILTNLATTSLMTPLPT
                     TISG"
     gene            4944..5879
                     /locus_tag="VGE92_10355"
     CDS             4944..5879
                     /locus_tag="VGE92_10355"
                     /EC_number="6.2.1.n2"
                     /inference="COORDINATES: protein motif:HMM:NF005479.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--[acyl-carrier-protein] ligase"
                     /protein_id="HEY1314271.1"
                     /translation="MSTASTTASAAVATPADPLAHIAAGLFRPMGVDGVYARTALYTN
                     LVERLEGYITRQRDPKAEIMRFPPVMSRTQLEKSGYLKSFPNLLGCVCALHGSEASIR
                     SAVDRYDNGGDWTTSVTSSDLVLSPAACYPVYPIVAARGQLPSGGLQFDIEADVFRHE
                     PSRSLDRLQSFRMREFVRIGSPQEILEFRESWMAKAPKLAADLSLPFNIDVANDPFFG
                     RVGQVMAVSQRQQALKFELLIPYYPTASPTACMSFNYHREHFGHVWGIKDDKGELAHT
                     SCVAFGIDRLAVALFANHGLDLAKWPVVTKAALAL"
     gene            5901..6839
                     /locus_tag="VGE92_10360"
     CDS             5901..6839
                     /locus_tag="VGE92_10360"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY1314272.1"
                     /translation="MPLDPRVKRFLDVLAAGKPPNMLETTVAERRSGLTELMKFGGPE
                     AAVGAIQDRTLPGADDLPLAVRVYTPLGATAGLLPGLVYFHGGGMVAGTVATHDSIGR
                     ALANSGACRVVSVDYRLAPEHPYPAALDDALAAVTYIGTHATEFGIDAERLGICGDSA
                     GGTLAAATAQAVARIGSPRLALQLLICPILDYSSSTPSKRDLGTGYLVDQATLDHDLL
                     YYAPPGTDPADPRISPLRAASVAGLPRTLIHTAEFDPLRDEGRDYYERLTRENAEVSY
                     TCHPGMIHLFYGLGAVIPYARTAFAQIGGEIRAALA"
     gene            6857..7240
                     /locus_tag="VGE92_10365"
     CDS             6857..7240
                     /locus_tag="VGE92_10365"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY1314273.1"
                     /translation="MAQRVVAERMIQQLHAARIGADLGGLCKLFADQGRFEIVGASAD
                     KPIAIRASGIAEFRPWLAMMVKVFRLTDYTLLSLVVEWPRATAHWRVDIYSKVTGVTI
                     PTELVDLVELSEDRILSYTEFFAPR"
     gene            7245..7868
                     /locus_tag="VGE92_10370"
     CDS             7245..7868
                     /locus_tag="VGE92_10370"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY1314274.1"
                     /translation="MDDPQWLVIGRELRAIAQTGLTFTKDRFDRQRYERLRELAATLL
                     AHGSGVDYASILGILRNDRGYATPKVDVRGAAFVEGKVLLVREISDGKWTLPGGWADI
                     NQSAAECVVREIAEESGFEARALKLAAVRDYQKSGHPPRNLDCIYKIFFICEITGGAA
                     RASDETSEAAFFPRDELPPLSLGRITAAQIERMFEHAEQPQLATDFD"
     gene            complement(7869..9170)
                     /locus_tag="VGE92_10375"
     CDS             complement(7869..9170)
                     /locus_tag="VGE92_10375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1314275.1"
                     /translation="MSLRGKLSLILTGLVSLVAALSVWAGHVVGSALLAWALVIGAVI
                     FPVLWLAAHIMRPIAQMLRALAGTVASYREGDFSLSLVADRDDELGELMTAHNELAAA
                     LRTQRAHLVQRELLLDTVMQNSPVALVLVDAHGRIAYANIAARHLLSEGRSLNGLRFA
                     DALARSPAALRAAAVQGDSLFSTEIDGVEETFHLSQRAFILQGQTFRLYLLKRLTREL
                     SRQEVTTWKKLIRVLSHELNNSLGPLSSLAHSGAEIARRGDYSTLPTVFAAIAERAEH
                     LHHFVGSYATFAKLPAPRPERIEWAAFLADLARQQTFTAAADLPAVAGWFDRVQVEQA
                     LINLLKNAYEAGGAKSEVQLKITCDGADQRIEVRDRGAGMTQSVLAQALLPFYSTKRS
                     GTGLGLALAREIAEAHGGRIQLANREGGGLSVVLDLPFAAP"
     gene            complement(9178..10536)
                     /locus_tag="VGE92_10380"
     CDS             complement(9178..10536)
                     /locus_tag="VGE92_10380"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY1314276.1"
                     /translation="MRTVLVIDDNPAVAQALQVLFGLHDIRTISALTPQKGLALLARE
                     PVDLVIADMNFSADTTSGEEGTALFRAVRASQPDLPVILLTAWTHLESAVQLVKAGAA
                     DYIGKPWDNDKLLATAENLLELAEATREVKRLQDEQRRRRERLAEQYDLCGVVFASDA
                     MQRVVELACRVARADVRVLITGPNGAGKERIAQIVHANSAIRNGPFVAINCGAIPAEL
                     IEAELFGVEAGAFTGANRVRAGRFELADGGTLFLDEIGDLPLAGQVKLLRVLETGQFE
                     MLGSSRTLSTRARIVSATNADLSVLIREGRFREDLYYRLNVIEIGVPPLAERAADILP
                     LARHFLGQTLQLSEDAAAALLAHDWPGNVRELRNSIERAKLLARGQVVTAADLNLPAA
                     QRQPAEAESTRRGIEPDPSREQIESSLREAGGNISRAAQSLGLSRQALYRRIERFRRP
                     LG"
     gene            complement(10559..11776)
                     /locus_tag="VGE92_10385"
     CDS             complement(10559..11776)
                     /locus_tag="VGE92_10385"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY1314277.1"
                     /translation="MELRPIVSAMRRNKFGALLIAAQMAVTLAFLANALTLIEQRLAL
                     SARPTGIDEADIFVMSTEAVDHPKHLAAHQAADLAALRSLGGVVDAYATNNYPLEGGG
                     SVQTVDLTANQQTPNSIAAFYFGDEHALHTLDLKLIAGRNFTADDVAERTANNIPPPS
                     GFIVTQALAARLFPGGDALGKSLYVELPTPSPIIGIVERFQSPFTSATGQLSAAAENS
                     LLVPYRMIAESSAYMVRAAPGHLDAVMKSAAAKLLELDGERILRSKSMRQLRADAYRG
                     NHGLVVLLGSICIALLAVTALGIVGLTSYWVAQRRRQIGIRRALGATQSAIVRYFQTE
                     NFLIGSAGALMGIALAIVLNLWMVSRFEMVRLHNSHAIGGALVILLLGQLAVLWPALR
                     AASIAPALATRGE"
     gene            complement(11782..>12506)
                     /locus_tag="VGE92_10390"
     CDS             complement(11782..>12506)
                     /locus_tag="VGE92_10390"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY1314278.1"
                     /translation="FYDLNLASFGQIDEMFVPFTRAIDRHMSAIGNTMCKDDTPVGWD
                     AFLASDCLWLQFWVELPTAGDVQQYRAFIHNYAAEQQRIGRFHWPAHTQLRNVMQWLA
                     YRDVVPSEVNILLLVSFGFLLVCLLNAMGLMLAKIMGRAGDIGVRRALGASRAAIFMQ
                     CVVETGIVGFAGGIFGLALTGLGLLAARSLLDKELIALTYLDWTDIGIAVFLAIAATI
                     VAGLYPTWRAAHVAPAWQLKAQ"
BASE COUNT         2361 a         3994 c         3870 g         2281 t
ORIGIN      
        1 gcgcggaaac tcacacgcgc acggctcccg ccgaaatcgc cgcaaacaac ggcgcatcga
       61 gcggcacgta agcctgcgat cgggaatcat cgaagtacaa gggatcgctg gtgcgggccg
      121 gatcgaatcc cgcctgcagc aaatcttgct tgcgcggctt gaacgttccg gtggattcga
      181 tgttcttcaa caaccgcagg aacaccggtc gcgcgtagct cggcagcttg agcgcgacat
      241 ccgcccggaa ggcggccaga tcgaagtccg cattgaccag cagcgccgcg agcccggcgc
      301 ggccgtcggc acccggcacc gcgacaccgt agacgacgcc gtcgacaacc ccgcgggccg
      361 cggtgagcgc ggtcaagacc tcggctgtcg atacgttctc gcccttccag cggtaagtct
      421 cacccacacg gtccacgaag taatagaaac cgcgttcatc gcggcgcatc agatcaccgg
      481 tgcgatacca ggagtcgccg tccttgaaga cattgtgcaa gaccttgcgc gccgttgctt
      541 cggggtcggc gtaaccttcg aatctgccgg caagttcctt gccgccggcg gggatcaagc
      601 cgactgcctc gccgacctcg ttggccaggc accgctcgca gaatccggcg tcgttgcgtc
      661 tgggctcgcc gcgctcgaca tcgaagcgca gcagcgcgac cggcaaacgg tgcgcaagaa
      721 acgacggtat cttgccgatc gccccgacac gcccctcgca gttgtagagc gagaaattgc
      781 cctcggtcga ggcgtagtac tcgaggattc gcgggatctt gaaacggttc tggaacggct
      841 cccacacctc cggacgcagg ccattgccgc aggcgatacg cagcgaatgc gaggtttcga
      901 ggtcctgatg cgctgcgttc aggaggtagc ggcacagttc gccgatgtat tgaaacaagg
      961 tgcagcgctc atcgcgcacg tcgcgccaga agtccgaagc cgaaaaacgc gcgcgaatga
     1021 tcacggcgcc gccgccgacc aatgtcgcgc cggtcgcgac cactccgccg acactgtggt
     1081 agagcggcag gcagttgaac atgcgatccg tcggcccggt atcgagcaag ccggcgaacc
     1141 aatggctcca ctgcatgacc ctatagtggc tgaccttggc tgccttcggc aggccggtcg
     1201 tgcccgacgt gtagatgtac agcgcagagc tgtcgagccg cggcggcgaa cattccgaat
     1261 cgcgcagttg ggcgcccgac aatcgtatga gctgcggcgc caggggtgcg aggtcgtgtg
     1321 cgctattccc gtacacccaa cacggcagcg cgctgcgcag ccgtgcacgc acctcggtga
     1381 gccgcgccgc gagttcgctg cccacgatca ccgcctgcgg cgcaacgatg ttgatcgaat
     1441 gcaccagcgc atcgcccgcg aggttcgtgt tgatcaacgc gacgaccacg ccgatgcgcg
     1501 tcagacccag ccagaccgcc atatattcgg cgcaattcgg catcagcagg cacaccgtgt
     1561 cgccggcatt caagccctgc ccaattcccc agcgcgcgta ttggttgcag cgattcgcga
     1621 gttcccgata gctcaggcag gcgtcattcg atagcagtgc gggcgccgca tcgaattggt
     1681 gcgccagccg atcgatcaat gccggcagcg tgagcgccgc ttgatcgatc gatgccgtgc
     1741 gctccagggc gcggatccag gcttcgcggg ctaatttggc gcccgggtcg gcggatgatg
     1801 ttacgggagt gctgctcatg gcgtcgctat ctttggaatt gcgaaccgta ccacgactgc
     1861 ccgaggcgtt cgagatacac tgttggcact ttttgcaacg agaatgcggc gggaatgaag
     1921 cgggaaacaa tcgcaattca cggtgggttc gactcggatc cggcgaccaa ggcggtggct
     1981 gtgccgatat atcaaaccgc cgcgtactca ttcgatagcg ccgaacacgg cgcggcgcta
     2041 ttcaacctgg aagtgccggg ctatcgctac acccggatca gcaatccgac aaccgacgtt
     2101 ctcgagcaac gcgtcacaga actcgaaggc ggcgtcggca gcatcagtgt gagctccggg
     2161 caaacggcgc tgtattacgc tacggccaac ctcacggaaa tgggccgcaa catcgtctcg
     2221 gtgccgcagc tctatggaac gacctacacc ctgttcgcgc atgtcctgcc gaaaatgggc
     2281 gtgcaggtgc gcttcgcgaa gtccgaccgc gcccccgatc ttgaagcctt gatcgacgcg
     2341 gacacgcgcg ccatattttg cgaaaccgtc ggcaatcccg ccggcaatgt ctgcgacctc
     2401 gaagcgcttg ccgatgtcgc acaccgtcat cgcatgccgc tgatcgtcga caatacggtg
     2461 gcgacgccga tgttggtgcg tcccaaagaa tacggcgcgg atatcgtcgt gcattccttg
     2521 acgaaattca tgggcggtca tgggaccacg ctcggcggca tcgtcgtcga ttgcggcact
     2581 ttctcgtgga ctgacaatgc cagccgctat cccatgttta cgcagcccga tgagtcttat
     2641 catggacttg tgtacaccga gcattacggc gccgccgcat tcgtcggccg ttgccgcagc
     2701 gtctatcagc gcaccctggg cgcagtgctc tcgcccatga acgcgttctt gctcctgcag
     2761 ggcatcgaaa cggtcgcgct gcgggtcgaa aggcatgtcg agaatgcgcg caaggtggcc
     2821 gagttcctgc gcgccagtcc gcgcgtcgag tcggtgaatt tcgcggggtt cgaagacaat
     2881 ccgtatcatc cgctggtacg gaaatacctc ggcggcaagg catgttcgct catgaccttc
     2941 gagattcgcg gcggcctcgc ggccggcatg cgtttctaca atgcactcaa gctattcaag
     3001 cgcttggtga atctcggcga tgcgaagtcg ctcgcctgcc atcctgcctc gacgactcac
     3061 cggcagatgt cgcccgccga acagctggcc gcgggcgtgg cccccggcgg cattcgcttg
     3121 agcgtcggaa tcgagcatat cgatgacatt ctggccgatc tcgaccaggc gctgcgagtt
     3181 gccgtcggct agacggcgga cggccgctag gcgtctttgc gctgcgcgag cgtacgcacc
     3241 accagcgcct ggatcgcagc cacggagtgg aagttttcgg gagtgatatc cgactgcggg
     3301 atggcaatat cgaattccag ctcgacagcc agcatcagat tcaccatttt cagcgaactg
     3361 ataccaaggt cgctcagctg ttggtcgacc ggaaaggggt ccgggacgac cgcagtcgcc
     3421 cccagcgtct gttttaccag cgccacgagc tgtgggccta ccccttgaat cgtcttctga
     3481 tccactcgat acatctccca gaggaatgcc gcccgatcat acgtgagccg gggctcgatg
     3541 ttgagacgag attgacaacg atagtttgta gcgtgtcaca caatacattc aaagttttac
     3601 atatgcttat gaaagctggg ccggacgtcg atggggcgcg caattccgcc aaattagcgg
     3661 ttgtccgccc tggtgactct ccgcataatg acccggagaa tgcggtaaat ccacggttag
     3721 aggatgttta atggaacctc agagaatgtc gggcgctgcc cgggtagtga atgttcgcca
     3781 aaacgatttg actgcgcgcg cacgtgccgc cgccgacgtc gcgaaagcga acgccgcggc
     3841 ggtcgacgcg gaggcgcgct ttcctgccga gtcgttcgcc gaaattcgta agcagcggct
     3901 gctcggtatc caggtacccc agtccctggg cggcgaaggc gcgagcctcg cggaaatcgc
     3961 cgacgtatgc tacatcctcg gccagtcgtg ctcctccacc gcgctgatct atgccatgca
     4021 ccaaatcaag atggcctgca tggtgcgcca ctgcaagggc aattccgcga ttgaacgaat
     4081 tttgagccgc atcgccaacg agcagctgct catggcctcc tccacgaccg aaggcacggc
     4141 cggcgggaac gtgcgctcga gcgaggctgc cgtggtgcac gaaggcgcgc aagtgaccct
     4201 cgagcgcaaa gccacggtca tttcgtatgc cgtcgaggcg gacggcgtgg tgaccacggc
     4261 gcgccgggcg gccgatgcgg cgggctcgga ccaggtgctg ttggtgttgc tcaaggcgga
     4321 ttacacgctc gaacggctgc aagtgtggga cacgctcggc atgcgcggca cgcatagcga
     4381 gggcttcacg ctgcgcgcac gggcggcggc cgagcagatc atgcccgaac cctacgatcg
     4441 aattcacgct cagaccatgg tgccctttgc gcatctctta tggggatcgg tgtgggcggg
     4501 aatcgcggcc gcggcggccg gcaaggcaca ggccttcatc cgcatggcgg tccgcaactc
     4561 gagtggccaa ttgccgcccg gtgcggcgca cttcacgcag gcggtctcga cgctgcgcac
     4621 cttacgcggt gtgttgagcg ccaatcttcg cagctacgaa gcgatcatgg gcgatgacaa
     4681 agcgatatcg tcgttggagt ttcaggcgat gatcacgctg accaaggtgc aggtgtcgga
     4741 actcgcggtg acgacggtgt tgagctcgct gcgcgcctgc ggattgtcgg gctaccgcaa
     4801 cgacaccgag ttcacgatcg gccggctgct gcgcgatgtc ctgtccgcgc ctctcatgat
     4861 caacaacgat cgcattctca ccaatctggc aacgacgtcg ttgatgacgc cgctgccgac
     4921 taccatcagt ggttaatact cctatgtcta ctgccagtac taccgcctcc gctgccgtcg
     4981 ccacccctgc ggacccgctc gcgcatatcg ccgcaggatt gtttcgtccg atgggcgtcg
     5041 atggcgtgta tgcgcgcacc gccttgtata cgaatctcgt cgagcgtctc gaggggtaca
     5101 ttacgcggca acgcgacccg aaagcggaaa tcatgcgctt cccgccggtg atgagccgca
     5161 cgcaactcga gaaatcgggc tacctcaaga gcttccccaa tttgctgggc tgcgtgtgtg
     5221 cgttgcacgg ctccgaggcc tcgatccgat cggcggtcga ccgttacgac aacggcggcg
     5281 actggaccac gtcggtgact tcctcggatt tggtgctgtc gcccgccgcg tgctatccgg
     5341 tctatccgat tgtcgccgcg cgcggccagc tgccgtccgg cgggctgcaa ttcgatatcg
     5401 aagccgatgt attcagacac gagccgtcac gcagcttgga ccgtctgcag tcgtttcgca
     5461 tgcgcgaatt cgtgaggatc ggatctccgc aggagatctt ggagtttcgc gaaagttgga
     5521 tggccaaggc accgaagttg gcggccgact tgagtctacc cttcaacatc gacgtggcga
     5581 acgatccgtt tttcggccgt gtcggccaag tcatggcggt gagccagcgg cagcaagcgc
     5641 tcaagttcga attgttgatc ccgtactacc cgacggcctc gcccacggct tgcatgagct
     5701 tcaattacca tcgcgagcat ttcggtcatg tgtggggcat caaggacgac aagggcgaat
     5761 tggcgcatac cagctgcgtc gccttcggca tcgaccgctt ggcggtcgcg ctgttcgcga
     5821 accacggcct ggacctcgcg aagtggccgg tagtaaccaa ggctgcactg gccctgtaac
     5881 gccgcgccgg gaaggatttc gtgccgcttg acccgcgcgt taaacgcttc ttggacgtgc
     5941 tggcagcggg caagccgccc aacatgctcg agaccacggt cgctgagcgg cgctccgggc
     6001 tcacggagct catgaaattc ggcggtccgg aggctgcggt cggcgccatt caggaccgca
     6061 ccttgccggg agccgatgat ttgccgctgg cagtgagggt gtacaccccc ttgggagcga
     6121 cggcggggct tctgcctggt ctggtttact ttcatggggg cggcatggtg gcgggcaccg
     6181 tggcgacgca tgattccatc ggccgtgcgt tggcaaattc gggcgcgtgt cgggtggtct
     6241 cggtcgacta ccgccttgct ccggaacatc cctatcccgc cgccctcgac gacgccctgg
     6301 ccgcagtcac gtatatcgga actcacgcga ctgaattcgg tatagacgcc gaacgcctcg
     6361 gtatctgcgg cgactcggcc ggcggcacgc tcgctgcggc gaccgctcag gcggtggctc
     6421 gcattggcag tccgcgcttg gcgctgcaat tgctaatttg cccaattctc gattacagca
     6481 gttcgacccc ctcaaaacgc gacttaggca caggctacct ggtcgatcag gcgacgctcg
     6541 accatgatct gctgtattac gcaccgcccg gcacagatcc ggccgatcca cggatttcgc
     6601 cgctgcgcgc ggcaagcgtc gccggtctgc cgcgcacgct gattcacacg gccgaattcg
     6661 acccgctgcg cgacgagggc cgggattatt acgagcgcct cacccgcgag aacgcggaag
     6721 tgtcctacac ttgccacccg ggtatgattc atctgttcta cggccttggg gccgtgattc
     6781 cgtatgcccg aaccgcgttc gcacagatcg gcggtgaaat ccgggctgca ttggcctgac
     6841 cgaaggagag attaaagtgg cgcagcgcgt cgttgcagaa cgcatgattc aacaactgca
     6901 tgccgcgcgt atcggcgccg atctcggcgg gctgtgcaag ttgttcgcag accaaggccg
     6961 attcgaaatc gtgggcgcca gcgccgacaa gccgatcgcc atccgggcaa gcggtatcgc
     7021 ggaatttcgg ccctggctcg cgatgatggt caaagtattt cggctcaccg actacacctt
     7081 gttgtcgctg gtagtcgaat ggccgcgagc caccgcgcat tggcgcgtcg acatctactc
     7141 caaagtcacc ggtgtcacca ttccgacgga actcgtcgat ctggtggagc tgagtgaaga
     7201 ccgcattttg agctataccg aatttttcgc gcctcgctga gccggtggac gacccgcagt
     7261 ggctcgtgat cggccgcgaa ttgcgcgcca tcgcgcaaac cggcctcact ttcaccaagg
     7321 atcgcttcga ccgacagcga tatgaacgcc tgcgcgagct ggcggcgacg ttgctggcgc
     7381 acggctcagg cgtcgactac gcgtcgattc tcgggatttt gcgcaatgac cgcggctacg
     7441 cgactcccaa ggtcgacgta cgcggcgccg ccttcgtcga gggcaaagta ctgctggtgc
     7501 gcgaaatcag cgacggcaag tggacgctgc cgggcggctg ggcggacatc aatcaatccg
     7561 ccgccgagtg cgtggtgcgg gaaattgccg aagagtcggg gttcgaggcc cgcgcgctga
     7621 agcttgcggc cgttcgcgac tatcaaaaga gcggacatcc gccgcgcaat ctcgactgca
     7681 tttacaagat ctttttcatt tgcgaaatta ccggcggcgc ggcgcgcgcc agcgacgaga
     7741 cgagcgaagc ggcatttttc ccgcgagacg agctgccgcc tttgtcgctc ggtcggatca
     7801 ccgcggcgca gatcgagcgc atgttcgagc acgctgagca gccgcagctg gcgacggact
     7861 tcgactgatc aaggcgcagc gaaaggcaga tccaacacca cgctgagacc gccgccttcg
     7921 cgattcgcca attgaatgcg cccgccgtgg gcttcggcta tttcccgcgc gagcgccagt
     7981 ccaagtccgg ttccgctgcg cttggtcgaa tagaacggca gcagcgcctg tgcgagcacc
     8041 gactgagtca tgccggcacc gcgatcgcgc acttcgatgc gctgatccgc gccatcgcac
     8101 gtgattttga gctgcacctc gctcttcgcg ccgcccgcct catatgcgtt cttcagcaag
     8161 ttgatgagcg cctgctcgac ttgaacccgg tcgaaccagc cggcaacggc aggcaggtcg
     8221 gccgcggccg tgaacgtttg ttggcgcgcg agatctgcca ggaatgccgc ccactcaatc
     8281 cgttccgggc gcggtgccgg cagcttggcg aaggtcgcat agctgcccac aaagtggtgc
     8341 aggtgctcgg cccgttcggc aatggcggcg aacaccgtcg gtagcgtcga gtagtccccg
     8401 cgccgcgcga tttcagcgcc ggaatgcgcg agcgaagaca acggtcccaa ggaattgttg
     8461 agttcgtggc tcaaaacacg aatcagcttc ttccacgtcg tcacttcctg gcgtgacagc
     8521 tcgcgcgtca accgtttgag caagtacaga cgaaaagtct gcccttgtag gatgaaagcg
     8581 cgctgcgaca ggtgaaacgt ctcttcgact ccgtcgatct ccgtcgaaaa caagctgtcg
     8641 ccctgcaccg ctgcggcgcg cagcgcagcg ggcgagcgcg ccagcgcgtc cgcgaagcgc
     8701 agcccattga gacttcgtcc ctcgctcaac aagtggcgcg ccgcgatatt ggcgtaagcg
     8761 atccgcccgt gggcatcgac cagcaccagc gcgaccggcg aattctgcat gacggtgtcg
     8821 agcagcagct cgcgctgcac cagatgcgct cgctgggtac gaagcgccgc cgcgagttcg
     8881 ttatgagccg tcatcaattc accgagttca tcgtcgcgat cggcgaccaa tgacaagctg
     8941 aaatccccct cgcggtaact cgccaccgtg cctgcaaggg cgcgcagcat ctgtgcgatc
     9001 gggcgcatga tgtgcgcggc cagccacagg accggaaaga ttaccgcgcc gatcaccaac
     9061 gcccacgcaa gcagcgcgct gcccacaaca tgccctgccc agacgctgag cgccgccacc
     9121 aggctcacga gcccggtgag gatcaacgac agtttgccgc gcaggctcat tgcgggctca
     9181 gccgagcggc cgcctgaatc gctcgatgcg ccgatacagt gcctggcgtg acagcccgag
     9241 gctttgcgcc gcacggctga tattgccgcc cgcctcgcgc aggctgctct cgatctgctc
     9301 gcgactaggg tcgggttcga tgcccctgcg agtcgattcc gcttcggccg gctgccgttg
     9361 cgccgccggt aaattcagat ctgcggccgt gacaacctgc cctctcgcca ggagcttcgc
     9421 gcgctcgatg gaattgcgca gttcgcgcac gttgccgggc caatcatgcg ccaacagcgc
     9481 tgccgcggca tcctcgctca gctgcagcgt ctgcccgaga aagtgccgtg ccagcggcaa
     9541 gatatccgcg gcgcgttcgg ccaacggcgg tacgccgatc tcgatgacat tgagccggta
     9601 gtacaaatct tcgcggaatc gcccctcgcg aatcaatacc gaaagatcgg cattggtcgc
     9661 actgacgatt cgcgcgcgcg tgctcaaggt gcggctcgat cccagcatct cgaactggcc
     9721 ggtctcgagc acgcgcagca atttcacctg accggcgagc ggcagatcgc cgatttcatc
     9781 caggaacagc gtgccgccgt cggcgagctc gaagcggccg gcgcgcaccc ggttcgctcc
     9841 ggtgaaagcg ccggcttcga cgccgaacag ctccgcctcg atcaactcgg cgggaatcgc
     9901 gccgcagttg atggcgacga atggcccgtt gcgaatcgcg gaattcgcat gcacgatttg
     9961 cgcaatccgc tccttgcctg cgccgttcgg ccctgtgatc agcactcgga cgtcggcccg
    10021 cgccactcgg cacgcgagtt cgaccacccg ttgcatcgcg tcggacgcaa aaaccacgcc
    10081 gcacaagtcg tactgctccg ccagccgctc ccggcgccgg cgctgctcat cctgcagccg
    10141 cttgacctcg cgcgtcgctt cggccaattc cagcagattc tcggcggtcg cgagcagctt
    10201 gtcgttgtcc cacggtttgc cgatgtaatc ggctgcgccg gccttcacca gctgtaccgc
    10261 ggattccaag tgggtccacg ccgtcagcag gatgaccggc aaatcgggtt ggcttgcgcg
    10321 cacagcccga aatagcgctg tcccctcctc gcccgacgtg gtatcggcgc tgaagttcat
    10381 atcggcgatt acgaggtcaa ccggctcgcg cgcgagcagc gcgagaccct tctgcggggt
    10441 caatgcggaa atggtgcgga tatcgtgcaa gccgaacaac acctgcaatg cctgcgccac
    10501 cgccggattg tcatcgatga cgagtactgt gcgcatggcc caatgttatc gggttcgcct
    10561 actcgccgcg cgttgccagc gcgggcgcga tggacgcggc acgcagcgcc ggccagagca
    10621 ccgcgagctg gccgagcagc aagatcacga gggctccgcc gatagcatgg ctattgtgca
    10681 gacgtaccat ctcgaaacgg ctcaccatcc acaggttcaa cacgatcgcg agcgcaatcc
    10741 ccatgagcgc gccggccgaa ccgatcagaa aattctcggt ttggaagtat ctcacgatgg
    10801 cgctttgcgt ggcgccgagc gcgcggcgaa tgcctatctg gcgacggcgc tgcgcgaccc
    10861 agtagctggt gagtcccacg attccaagcg ccgtaaccgc cagcagcgcg atgcaaatcg
    10921 agcccaacag caccaccaat ccatgattgc cccggtaagc gtcggcgcgc agctgccgca
    10981 tggatttgct gcgcaagatc cgctcgccgt cgagctcgag aagcttcgct gcggcggatt
    11041 tcatcacggc atccaaatgg ccgggggcgg cgcgcaccat gtaggccgag gattctgcaa
    11101 tcatccgata gggcaccagc agcgaattct cggcggcggc gctcagttgt cccgtggcac
    11161 tggtaaaggg cgactgaaaa cgctcgacga tgccgattat gggcgacggc gtcggcagtt
    11221 cgacatacag ggattttccg agggcgtcgc cgccgggaaa taaccgcgcg gccagagctt
    11281 gtgtgactat gaacccgctc gggggaggaa tattgtttgc cgtgcgttcc gcgacgtcat
    11341 cggcggtaaa attgcgcccc gcaatgagct tcagatcgag cgtatgcagc gcgtgttcgt
    11401 cgccgaaata gaaggcggcg atcgaattgg gagtctgttg attggccgtg aggtccaccg
    11461 tttgcaccga gccgccgccc tccagcggat agttgttcgt tgcatacgca tcgaccactc
    11521 cgccaagcga acgcagtgct gctagatccg ccgcttgatg cgcagccaag tgctttgggt
    11581 ggtcgaccgc ctcggtcgac atcacgaaaa tatcggcctc gtcgattccg gtgggacgcg
    11641 cgctcaacgc cagccgctgt tcgatgagcg tcagcgcatt ggcgagaaac gccagggtca
    11701 ctgccatctg tgcggcgatg agcagcgcgc cgaatttgtt gcggcgcatg gcggacacaa
    11761 tcggacgtag ttccatggcc gtcactgcgc tttgagctgc caggccggcg ccacgtgcgc
    11821 ggcgcgccaa gtcggataca aacccgcgac gatcgtcgcc gctatggcca gaaagaccgc
    11881 gatgccgata tccgtccagt cgaggtatgt gagtgcgatc aattccttgt cgagcaagga
    11941 gcgcgccgcc aataggccaa gccccgtgag cgccagcccg aaaatgcccc cggcgaatcc
    12001 cacaattccg gtctcaacca cgcactgcat gaaaatggcg gcgcggcttg caccaagagc
    12061 gcggcgtacg ccgatgtcgc cggcccgtcc catgattttc gccagcatga gccccatggc
    12121 gttgagcagg cacacgagca ggaagccaaa tgagaccagc agcaaaatgt tcacttcgct
    12181 cggaacgacg tcacggtacg cgagccattg catgacgttg cgcaattgcg tgtgcgcagg
    12241 ccaatggaaa cggccgattc gttgctgttc ggcggcgtaa ttatgaatga aggcgcgata
    12301 ctgctgcacg tcgccggccg tcggcagctc cacccagaac tgcagccata gacagtcgga
    12361 ggccaggaac gcgtcccagc ccaccggcgt gtcgtccttg cacatggtgt tgccgatcgc
    12421 gctcatatgc ctgtcgatgg cgcgggtaaa cggtacgaac atctcgtcga tctggccgaa
    12481 ggacgccaga ttcaagtcat aaaaat
//
LOCUS       DASWZT010000368        11547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_85147, whole genome shotgun sequence.
ACCESSION   DASWZT010000368 DASWZT010000000
VERSION     DASWZT010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11547
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85147"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="VGE92_10395"
     CDS             <1..943
                     /locus_tag="VGE92_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314279.1"
                     /translation="FVAIISDNRSTAKLMISHRAAGEYEATPARIDKFKTTSIAPGVK
                     RKTSLAADMGALLGRRPHDINDLPQPHADLQPRESAARSVETNRLTIVIRSPFYVLLR
                     AHVRRRRCGFEMNELQEELRAVVDYISEQTGGLRMPGDRRARMALGCLDLAIEHQAGI
                     AVLAEQSLWGPAYALLRCMFDAFVRGVWLARCASEIELSSFEMAGLRGKPFRVLVDDV
                     ERVLGHSRGVLSKLRKSSWAMFSDFTHAGFEHVRRKPGVTTNDQDYPPAETDQALRLA
                     TALGLLSATELASLSGHRDIALACKARAELIASGRKH"
     gene            complement(1005..1080)
                     /locus_tag="VGE92_10400"
     tRNA            complement(1005..1080)
                     /locus_tag="VGE92_10400"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1044..1046),aa:Glu,seq:ctc)
     gene            1160..2557
                     /gene="pcnB"
                     /locus_tag="VGE92_10405"
     CDS             1160..2557
                     /gene="pcnB"
                     /locus_tag="VGE92_10405"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEY1314280.1"
                     /translation="MNASSTSFNPLIIPRSEHSISRAAISPNALKVLYRLKDAGYQGF
                     LVGGAVRDLLLGITPKDFDVATNALPDEVRRLFRNCRLIGRRFRLAHVHFGHEIIEVA
                     TFRAAAAPEREDVDEMDPDAPSEVSAQDGSEHRAFDQTGRILRDNIYGTIEEDVWRRD
                     FTANGLYYNIDDFSIWDFVDGVNDVRARRLKLIGDPETRYREDPVRMLRSVRFAAKLD
                     FSIEPKTEAPIGELAYLLDGVPPARLFDECLKLFLAGFGVKSFRLLRQYGLFEHLFPL
                     SAAAFALPPYSYAQEMLERGLSNTDERIAADKPVTPTYLFAVVLWSAVLRELNERQAG
                     PAPDLAQLMQACDTVLRAQQSRVAIPRRFAIPMRELLMLQPRFNRRSGVKSLSLLQHP
                     RFRAAYDFLLLRAEVGVADPELAKWWTDIQLLPQEERVALVQARPADPAEKTEGAATP
                     SRRRRRRRRGGAART"
     gene            2561..3052
                     /gene="folK"
                     /locus_tag="VGE92_10410"
     CDS             2561..3052
                     /gene="folK"
                     /locus_tag="VGE92_10410"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEY1314281.1"
                     /translation="MSALWRPAYVAIGSNLDQPEARVREAFERLAASPAMRLVARSRL
                     YETQPMGPQDQPNFVNAAAGILSQLPPRELLTRLLGIETAMGRNRRERWGPRVIDLDL
                     LWMPGESIAEEGITVPHPGVSTRNFVLYPLADIAPTLSIPGIGRVLELLRSAGSDGIS
                     VLE"
     gene            3068..3733
                     /locus_tag="VGE92_10415"
     CDS             3068..3733
                     /locus_tag="VGE92_10415"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010158266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEY1314282.1"
                     /translation="MASAKPHRFIVVEGPIGVGKTSLAKRLSQSLGGQLILEQADQNP
                     FLERFYKNPRAAAFQTQLFFLFQRSRQLEDVRQEDLFGAVRIADYLLEKDRLFARVTL
                     DDAEYALYERVYERVVVDAPKPDLVVYLQAPVDVLVDRIAKRGIRYEQHIEHSYLDKL
                     NQAYARFFHSYDASPLLIVNSTAIDPINNDGDYQQLFAEISKTRRGRHFFNPAGSSLL
                     PSG"
     gene            3787..4623
                     /gene="panC"
                     /locus_tag="VGE92_10420"
     CDS             3787..4623
                     /gene="panC"
                     /locus_tag="VGE92_10420"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY1314283.1"
                     /translation="MEFLTRSASVRDRVRVWRAAGARVALVPTAGTLHKGHMHVVAEA
                     QERADHVIVSIFANPREREAPTLGADRELLLKMGATVAFAPPQQEIYPIGRELAASVT
                     VPALADMLEGTQRPGHFADLTTLLVKLFNVIKPDVALFGERDFQQLVIMRRLVDDLFL
                     SIEILGCATLRDSDGLAVASANRSLTAQERALAPRLHATLNEFVAKIDAGARDYEALQ
                     RQGMDALKAAGFAPEYFAIRQAADLGQVQCAARDLVILAAARLNAHRLIDNSRVRLID
                     RF"
     gene            complement(4582..5691)
                     /gene="queG"
                     /locus_tag="VGE92_10425"
     CDS             complement(4582..5691)
                     /gene="queG"
                     /locus_tag="VGE92_10425"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEY1314284.1"
                     /translation="MRKPNHLKTPIDPAALLTDLREWGRQLGFAHLGVARLDLARDEA
                     HFLDWLRAGFNGEMTYMSRHGVKRSRPAELKPGTVSCISVRMNYWPPAADAAATLADR
                     NLGYVSRYALGRDYHKVMRSRLQKLCDRLEGAVGPFGHRVFTDSAPVLEKALARNAGL
                     GWIGKHTNLIDRDAGSYFFLGEIYLDLELPSEQASSAHCGSCTACIPACPTAAIVAPY
                     RLDARRCISYLTIELAGAIPVEFRRAIGNRIFGCDDCQLVCPWNKFARPTAEKDFAVR
                     HGLDHSQLIDLFEWSEPQFLERTAGSAIRRIGYERWLRNIAVALGNAPTSAALLESLH
                     RRAQHPSSVVREHVQWALAQHSSKPVDEAHPRVVD"
     gene            5790..6272
                     /gene="tsaE"
                     /locus_tag="VGE92_10430"
     CDS             5790..6272
                     /gene="tsaE"
                     /locus_tag="VGE92_10430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEY1314285.1"
                     /translation="MPDSRATELLGESLARTLPGPGTSGAVVYLQGELGAGKTCCVRS
                     FLRALGVTGLVRSPTYTLVETYTVGSVTCVHVDLYRLKALTEVDELGLDWVQARCLLV
                     EWPDKGQGALPPADLVLALCYAGEGRRARLTAQSPLAASWLEKLGRDTSLSPYVSNLT
                     "
     gene            6307..7512
                     /locus_tag="VGE92_10435"
     CDS             6307..7512
                     /locus_tag="VGE92_10435"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY1314286.1"
                     /translation="MNILLPRPVWAMLLSMLMLPAHAVDVRAIRLWAGPDSTRVVLDL
                     SGSAQHNLQVLKNPDRVVLDVANARLASGARTALATGSVKQLRVARRPSGELRIVLDL
                     ARPIQAKSFLAVPNAHYGYRLVIDLGSNQSSETPVKAEHAPPDARDLIIAVDAGHGGD
                     DPGAIGKNGTREKDVVLAIARELALKISAEPGMRAVLTRNGDYFVPLRDRMRRARAQQ
                     ADLFVSIHADSIRDRRVDGSSVYILSQRGATDEASRWLAERENASDLIGGVSLDDKDD
                     VLASVLLDLSQSASLSASQVAAERVLRQLNRVGEVRKPQVQQARFMVLKSPDIPSMLV
                     ETAYISNPQEEMRLRGAAHQAKLAAAIHQGVRDYFYANPPAGTRIALLASGAPTARPV
                     MATAGDSGE"
     gene            7572..9368
                     /gene="mutL"
                     /locus_tag="VGE92_10440"
     CDS             7572..9368
                     /gene="mutL"
                     /locus_tag="VGE92_10440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEY1314287.1"
                     /translation="MPILLLSDALISQIAAGEVIERPASVVKELVENALDARAQRIEV
                     ELERGGCGLIRVRDDGSGIAPEEIALALARHATSKIASLDDLESVATLGFRGEALPSI
                     ASVSRLSLVSRSAASAHAWAVEVRDGSVAAPVPAAHPPGTTVEVRELFFNVPARRKFL
                     RSEATEYQHIVRMLERLALSRFDVAFSLAHNGKTVWTLAAAHSAEQRLARVAKLCGED
                     FAGHVIELKHDTETLRLSGWLALPTFSRSQADLQFAFLNGRFVRDKLLAGAVRLAYQD
                     VLFHGRFSAYLLYLELDPTAVDVNAHPQKLEVRFRDPRRIHEFVFRTLERVLAETRPS
                     VESAGSAPLDWLDLSAQFERPGAPNQAHIALPEGRSARRAEDAYRSMIAPTEYAVAEP
                     ARDPGRNEPSGNGAAPLGYAIAQLHGVYILAQTAEGMVLVDMHAAHERVMYERMKKLL
                     AGETVQQQLLIPEILHVTPAQAEAGESHAQEFAALGFGVTRLAPDQLALRSIPSLLAG
                     RDPARIVRDVLSDLLEQGHSRRVEESINHLLATMACHAAVRAQRNLTLPEMNALLREM
                     EVTDRADQCNHGRPTWVRLSLTDLDRLFLRGR"
     gene            9365..10348
                     /gene="miaA"
                     /locus_tag="VGE92_10445"
     CDS             9365..10348
                     /gene="miaA"
                     /locus_tag="VGE92_10445"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEY1314288.1"
                     /translation="MMGATSGRRAILLMGPTGSGKSDLAVQLAAALPVEIVSVDSALV
                     YRGMDIGTAKPSAALRARVPHHLIDIRDPVEHYSAGDFTRDAVASMQAIWARGRHPLL
                     TGGTMLYFHALSHGIAKLPAADPKLRAEIDAQAASGGWAALHRELAEVDPGAASRIHV
                     NDPQRIQRALEVYRLTGETITKLQQNRVSVFADVAVTEIAVAPLERSDLHTRIELRFD
                     AMLAAGLLAEVRRFFERSDLTAEHPSMRAVGYRQIWRYLAGHCALSEARDQAIAATRQ
                     LAKRQLTWLRRREHAQWFDSMHSDVARALMNALSQGGFALSSGVTPQGVLC"
     gene            10428..10685
                     /gene="hfq"
                     /locus_tag="VGE92_10450"
     CDS             10428..10685
                     /gene="hfq"
                     /locus_tag="VGE92_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEY1314289.1"
                     /translation="MARGQSLQDPFLNALRKERVPVSIYLVNGIKLQGQIDSFDQFVV
                     LLRNSVSQMVYKHAISTVVPARNVRLPTGEDGEVTEPAIVE"
     gene            10785..>11547
                     /gene="hflX"
                     /locus_tag="VGE92_10455"
     CDS             10785..>11547
                     /gene="hflX"
                     /locus_tag="VGE92_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY1314290.1"
                     /translation="MGVGIGHPVDPNDTAEFAALAASAGAIAVATVLASRARPDPKYF
                     VGSGKAEEIRSCAQLNAADVVLVDQTLSPSQERNLEKLTGCRVLDRNGLILDIFAQRA
                     RSFEGKLQVELAQLSHLATRLVRGWTHLERQKGGIGLRGPGETQLETDRRLIAKRIRT
                     LKARLEKLGRQRDTSRLVRREVPVPTVALVGYTNAGKSTLFNALTGAETYVADQLFAT
                     LDPTVRRVKLPGIGEVVLADTVGFVRALPHELVAAF"
BASE COUNT         2039 a         3550 c         3784 g         2174 t
ORIGIN      
        1 attcgttgca atcatcagcg ataaccgttc aaccgcgaag cttatgattt cccatcgagc
       61 cgccggcgaa tatgaagcga ccccggcccg gatagataag ttcaaaacga cttcgatcgc
      121 tcccggagta aaacggaaaa ctagcctggc cgctgacatg ggtgccttgc tgggtcgtcg
      181 gccccacgat atcaatgact tgcctcaacc ccatgccgat ttgcaaccgc gtgaatcggc
      241 tgcccggagc gtcgaaacta atcgtttgac catagtgatt cgctcgccat tctatgttct
      301 attacgagcg catgttaggc gccgtcggtg tgggttcgaa atgaatgaac tgcaggaaga
      361 gttgcgggcc gtcgtcgatt acatctctga gcagaccggc ggattgcgga tgccggggga
      421 tcgacgtgcc cggatggcgc tcggatgtct tgacctcgcc atcgagcatc aggcggggat
      481 tgcagtcctc gcggagcaat cattgtgggg accggcttat gcgctgctgc gctgtatgtt
      541 cgatgccttc gtgcgcggcg tctggctcgc gcgctgcgcc tcggagatcg aattgtcgtc
      601 tttcgagatg gcggggcttc ggggaaaacc ctttcgggta ttggtggatg atgttgaacg
      661 ggtgttaggg cattcccgcg gcgtgctgtc gaaactgcgc aagtcatcct gggccatgtt
      721 cagcgacttc acgcacgccg gattcgagca cgtcaggcgc aaacccggcg tcacaacgaa
      781 cgaccaggac taccctccgg ccgagaccga tcaggcgctg cgcctggcaa cggcgctcgg
      841 cctgttgtcc gcgaccgaac ttgcctccct ctccggacat cgcgacatcg cgctggcctg
      901 caaggcgcgt gcggagctga tcgccagcgg ccgcaagcat taggttcgac gagactacgc
      961 atttgattaa tcccataagg gattgatttt tatgcactct caattggcgc tccctacgag
     1021 attcgaactc gtgtacttgc cttgagaggg caatgtccta ggcctctaga cgaagggagc
     1081 gttgttaacc gggcgccccg gctcacgggg gctggtagta tacgggccaa tttttttgac
     1141 tcaagcggaa cccccctttt tgaacgcatc gtcgacgtcc ttcaacccgc tgatcatccc
     1201 tcgttcggag cactcgatct cgcgtgcggc gatctcgccg aacgccttga aagtcctgta
     1261 ccgcctgaaa gacgcgggct accaaggctt cttggtcggc ggcgcggtgc gcgacctgct
     1321 gctcggcatt acgccgaagg atttcgacgt ggcaaccaac gccttgcctg acgaggtgcg
     1381 gcgcctgttt cgcaattgcc gcctcatcgg ccggcgcttc cggctggcgc atgtgcactt
     1441 cggccacgag atcatcgagg tcgccacctt ccgcgccgcg gcggcgccgg agcgcgagga
     1501 cgtggatgaa atggatccgg atgcgccgag cgaagtctcc gcgcaagacg gctcggaaca
     1561 ccgtgcgttc gatcagaccg gccgcatcct gcgcgacaat atttacggca ccatcgaaga
     1621 agacgtatgg cgccgcgact tcacggccaa tggcctgtac tacaacatcg acgatttttc
     1681 gatttgggat ttcgtcgatg gtgtcaacga tgtgcgcgcg cgtcgcttga agctgatcgg
     1741 cgatcccgag acgcgctacc gcgaagatcc ggtccgcatg ctgcgctcgg tgcgtttcgc
     1801 cgcgaaactc gatttctcca tcgagccgaa gaccgaagcg cccatcggtg agctcgcata
     1861 tctgctcgac ggtgtgccgc cggcgcgatt gttcgatgag tgtttgaaat tgttcctggc
     1921 gggattcggc gtgaaatcct ttcggctgct gcggcaatac gggctgttcg agcatctatt
     1981 tccgttgtct gccgcggcgt tcgcgttgcc gccctacagt tatgcacaag agatgctcga
     2041 gcgcggcttg agcaacaccg atgaacgcat cgcggcggac aagccggtga cgcccaccta
     2101 tctgttcgcg gtcgtgttgt ggagcgcggt gctgcgtgaa ctcaacgaac ggcaggcggg
     2161 tccggcgccc gatctcgcgc agctcatgca ggcgtgcgac acggtgctgc gcgctcagca
     2221 gtcccgcgtc gccataccgc ggcgcttcgc gataccgatg cgcgaactgc tgatgttgca
     2281 gccgaggttc aaccggcgca gcggcgtcaa atccttgagc ctgctgcagc atccgcgctt
     2341 tcgcgcggcc tacgatttcc tgttgctgcg ggcggaggtc ggcgtggccg acccagaact
     2401 cgcgaaatgg tggaccgaca ttcagctgct gccgcaggag gagcgcgtgg ccctggtgca
     2461 ggcccgtccg gcggatccgg ccgagaagac cgagggcgcg gcgactccaa gccggcggcg
     2521 ccggcggcgg cggcgcggcg gagcggcgcg cacctaacct atgtccgcgc tgtggcggcc
     2581 cgcgtacgtg gcgatcggca gcaatctcga ccaacctgag gcgcgcgtgc gcgaggcgtt
     2641 cgagcgcctg gcggcctcgc ccgcgatgcg gctcgtggcg cgctcgcgcc tgtacgagac
     2701 gcagccgatg gggccgcagg atcaacctaa cttcgtcaac gcggccgccg gaatactcag
     2761 ccagttgccg ccccgcgagc tgctcacgcg attgctcggc atcgaaacgg cgatgggccg
     2821 caatcggcgc gagcgctggg gtccgcgtgt catcgatctg gatttgctgt ggatgccggg
     2881 cgagagcatc gccgaggagg ggatcaccgt gccgcatccc ggggtgtcga cccgcaactt
     2941 cgtcctgtat cctctggcag atatcgctcc cacgctttcg attccaggaa ttggccgcgt
     3001 gctggaactg ttgcggagcg cgggcagtga tggcatttcg gttctcgaat aggatcaaga
     3061 gtagtcaatg gcgagcgcga aacctcaccg gttcattgtg gtcgaaggtc cgataggtgt
     3121 cggtaaaaca agcctcgcga agcgcctgag ccagtcgttg ggcggccagc tgatcctcga
     3181 gcaggcggat cagaacccgt tcctcgagcg cttctacaag aacccgcgcg cggcggcatt
     3241 ccagacgcaa ctgttctttt tgttccagcg ctcccgccaa ttggaggatg tgaggcaaga
     3301 agatttgttc ggcgcggtgc gcatcgcaga ctacctgttg gaaaaggacc ggttgtttgc
     3361 ccgcgtcacc ctggatgacg cggagtacgc gctgtacgag cgggtctatg agcgggtggt
     3421 ggtggacgcg ccgaagcccg atctggtcgt ctacctgcag gcgccggtcg atgtgctcgt
     3481 ggatcgaatc gccaagcgtg gtattcgcta cgagcagcac atcgagcaca gctatctcga
     3541 caagctcaac caggcgtatg cgcgattttt tcatagctat gacgcctcgc ccctgttgat
     3601 cgtcaattcc acggccatcg acccgatcaa caacgacggc gactatcagc agctgtttgc
     3661 cgaaatcagc aagacgcgcc gcggccggca tttcttcaat cccgccggca gcagcctgtt
     3721 gccgtccggc tgacgagacc gcccgcgggc ggctaaaggt ctcggcttcg ttgccgatgc
     3781 atgggcatgg aattcctcac tcgcagcgcc tcggttcggg atcgggtccg ggtatggcgc
     3841 gccgcgggcg cgcgcgtggc gctggtgccc accgcgggca ccctgcacaa gggacacatg
     3901 cacgtggtgg cggaagccca ggagcgggcc gaccatgtga tcgtcagtat tttcgcgaat
     3961 ccccgcgagc gcgaggcgcc gacgctcggg gcggatcgtg aattgttgct gaagatgggt
     4021 gccacggtgg cgtttgcgcc gccgcagcag gaaatctatc cgattggccg ggaactcgcg
     4081 gcgtcggtga ccgtaccggc gctcgccgac atgctcgagg gcacgcaacg gccggggcac
     4141 ttcgccgacc tgaccacgtt gctggtcaag ttgttcaatg tcatcaaacc cgatgtcgcc
     4201 ttattcggcg agcgcgattt ccaacagttg gtcatcatgc gccgcctggt cgacgacctg
     4261 ttcctgtcga tcgagattct cggttgcgcg accctgcggg acagcgatgg actcgcagtc
     4321 gcctccgcca atcgaagctt gaccgcgcag gagcgcgcgc tcgcgccgcg gctgcatgcg
     4381 actttgaacg agttcgtcgc caagatcgat gcgggcgcgc gcgattatga ggctttgcag
     4441 cggcagggga tggacgcgct caaagcagcc ggcttcgccc ccgagtattt cgccatacgt
     4501 caggccgcgg atctcggtca ggtgcaatgc gcggcacgcg acctcgtgat cctcgcggca
     4561 gcgcgcctca acgcgcaccg gctaatcgac aactcgcggg tgcgcctcat cgaccggttt
     4621 tgacgagtgc tgcgcgagcg cccattgcac gtgctcgcgg accacgctcg agggatgctg
     4681 cgcgcgccga tgcagcgact cgagcagcgc cgccgaggtg ggcgcattgc cgagcgcgac
     4741 ggcgatattg cgcagccatc gctcatagcc gatgcgccgg atcgcgctgc cggcggtgcg
     4801 ctcgagaaat tgcggctcgc tccactcgaa caggtcgatg agctgtgaat gatcgaggcc
     4861 gtgccgcacg gcgaaatcct tctcggcggt gggacgcgcg aacttattcc acgggcagac
     4921 caactgacag tcatcgcaac cgaagatgcg attgccgatc gcgcggcgaa attccacggg
     4981 aatcgcgccc gccaactcga tggtgaggta ggagatgcag cgccgcgcat ccagacgata
     5041 gggcgcgaca atggccgcgg tcgggcaggc cgggatgcaa gccgtgcagc tgccgcaatg
     5101 cgcgctgctc gcctgctcgc tcggcaactc gagatccaag tagatttcgc ccaggaagaa
     5161 gtacgagccg gcgtcgcgat cgatcagatt ggtgtgtttg ccgatccagc ccaacccggc
     5221 attgcgcgcc agcgcctttt ccaggaccgg cgcgctgtcg gtaaacacac ggtggccgaa
     5281 cggaccgacc gcgccctcga ggcggtcgca gagcttctgc aaacgcgagc gcatgacctt
     5341 gtgatagtcg cgcccgaggg catagcgcga aacatagccg agattgcggt cggcgagggt
     5401 ggctgcggcg tcggctgctg gcggccaata attcatgcgt acgctgatgc agctcaccgt
     5461 gcccggctta agttccgccg gcctgctgcg cttcacgcca tggcgcgaca tataggtcat
     5521 ttcgccgttg aagcccgcgc gcaaccagtc gagaaaatgc gcctcgtcgc gcgccaaatc
     5581 gaggcgggca acgccgagat gcgcgaagcc gagctgccgg ccccactcgc gcaagtcggt
     5641 caacagggcc gcggggtcga tgggcgtctt gaggtggttt ggtttacgca tgcgccaacc
     5701 gaatttcggt tggcccgtat aatggcgaaa aacgccgcgg gccgctccgc caatggacag
     5761 ctatgcctcg agcctgcctc gatttcgact tgcctgacag ccgtgcgacc gagctgctgg
     5821 gagagtcgct ggcgcgaact ttgcccggcc ccggcacttc gggagcggtg gtgtatctgc
     5881 aaggcgaact cggcgccgga aagacttgct gcgtacgcag tttcctgcgc gcgctcggcg
     5941 tcaccgggct ggtgcgcagt ccaacctaca ccctggtgga gacctacacc gtgggttctg
     6001 tcacctgcgt ccacgtcgac ctgtatcggc tgaaggccct cacggaagtc gatgagctgg
     6061 gacttgattg ggtgcaagcg cggtgtctgc tggtggaatg gcccgacaag ggccaaggtg
     6121 cgctgcctcc cgcggatctc gttttggcgc tgtgttacgc gggggagggg cgccgggcga
     6181 ggctgaccgc ccagtcgccg cttgcggcga gttggctcga gaagctagga cgcgacacta
     6241 gcttaagtcc ctatgtatct aatttaactt gaaaaagatc ttgcatatgc gttaacttaa
     6301 tgcgccatga atattctgtt gccgcggccg gtatgggcga tgctgttgtc gatgctgatg
     6361 ctgccggcgc atgcggtgga tgtgcgggcg attcgtctct gggcgggccc ggatagcacg
     6421 cgggtcgtat tggacctgtc gggcagcgca cagcacaatc tgcaggtcct gaaaaatccc
     6481 gatcgcgtgg tgctcgacgt cgcgaatgcg cgcttggcga gcggtgcgcg tacggcgctc
     6541 gccaccggct cggtgaagca gctgcgggtg gcccggcgcc cctccgggga actgcgcatc
     6601 gtgctcgacc tcgcgcggcc catccaagca aagagcttcc tcgccgtgcc gaacgcccat
     6661 tacggttatc gattggtcat cgacttggga tcgaatcaga gcagcgaaac gccggtgaaa
     6721 gccgagcacg caccgccgga cgctcgcgac ctcatcattg ccgtcgacgc cggtcacggc
     6781 ggcgacgatc cgggcgctat cggcaaaaac ggcacccgcg aaaaggacgt ggtactggcg
     6841 atcgctcggg aattggcgct caaaatcagc gcagagcccg ggatgcgggc ggtgctcacg
     6901 cgcaacggcg attacttcgt gccgctgcgc gaccggatgc gtcgcgcgcg agcccagcag
     6961 gcggatctgt tcgtgtccat tcatgccgat tcgattcgcg atcgacgcgt cgacggctcc
     7021 tcggtttata tcctgtcgca gcgcggtgcg accgatgagg cctcgcgctg gctggcggag
     7081 cgtgagaatg cgtcggatct gatcggcggc gtgtcgctcg atgacaagga cgatgtgctc
     7141 gcctcggtgc tgctcgactt gtcgcagtcg gcttcattga gcgccagtca agtcgcggcc
     7201 gaacgggtgc tgcgccaatt gaaccgggtc ggcgaggttc gcaaaccgca agtacagcag
     7261 gcgcgcttca tggtgctgaa gtcccccgat ataccgtcga tgctggtcga gaccgcgtac
     7321 atctcgaatc cgcaagagga aatgcgcctg cggggggccg cgcatcaggc caagcttgcg
     7381 gccgccatcc atcagggcgt gcgtgactat ttctacgcca atccgccggc aggcacacgg
     7441 atcgcgctac tcgcgagcgg agcgccgaca gcacggccgg taatggccac cgcgggcgat
     7501 tcgggggaat agcgccgcgt acaccggcgc gactcgggct gtgcgaaact gcgcggctaa
     7561 cgaggtcgcc cttgcccatt ttgctgctct ccgatgccct catcagtcaa atcgctgccg
     7621 gcgaggtgat cgagcggccg gcgtccgtgg tcaaggagct ggtcgagaac gcgctcgatg
     7681 cacgggcgca gcgcatcgag gtggagctgg aacgcggcgg ttgcggcttg attcgcgtgc
     7741 gcgacgacgg cagcggcatc gcacccgagg agattgcctt ggcgctggcg cgccacgcca
     7801 ccagcaaaat cgcctcgctc gatgatctgg agagtgtggc tacgttggga tttcggggcg
     7861 aggcgctgcc cagcatcgcc tcggtctcgc gattgtcgct ggtgtcgcgg tcggcggcgt
     7921 ccgcgcatgc ctgggcggtc gaagtccgcg atgggtccgt cgcggcgccg gttcccgcgg
     7981 cgcacccgcc cggaaccacg gtcgaagtgc gcgagctgtt cttcaacgtg ccggcgcgcc
     8041 gcaagtttct gcgcagcgag gccaccgaat accaacacat cgtgcgcatg ctggaacggc
     8101 tcgcgctgtc gcggttcgat gtggcgttct ctctcgcgca caatggcaaa acggtctgga
     8161 cgcttgccgc ggcgcacagc gcggagcagc gcttggcgcg cgtcgcaaag ctatgcggcg
     8221 aggatttcgc cggccacgtg atcgagctca agcacgacac cgagaccctg cgcttgtccg
     8281 gctggctcgc gctgccgacc ttttcgcgca gtcaggcgga cctgcagttc gcgtttctca
     8341 acggccgctt cgtgcgcgac aagctattgg cgggcgcggt gcgtctcgcg tatcaagatg
     8401 tgctgtttca cggccgattt tccgcttatc tgctgtattt ggagttggac ccgactgcgg
     8461 tcgatgtgaa cgcccacccg caaaaattgg aagtgcggtt tcgcgatccc cggcgcattc
     8521 atgagttcgt gttccgcacg ctcgaacggg tgctcgccga gacgcgcccg agcgtcgaat
     8581 ccgcaggcag cgcgccgctc gattggcttg accttagcgc gcaattcgag cggccaggag
     8641 cgcccaacca ggcgcacatt gccctgccgg agggccgttc ggcgcggcgt gccgaggatg
     8701 cctatcggag catgatcgcg cccacggaat atgcggtggc ggagcccgct cgggaccctg
     8761 ggcgcaatga accgtccggc aacggcgcgg cgccattggg ctatgcgatc gcacagctgc
     8821 acggtgtgta tatcctggcg cagaccgccg agggcatggt cttggtcgac atgcatgccg
     8881 ctcatgagcg ggtgatgtac gagcgcatga aaaagctgct cgcgggcgag acggtgcagc
     8941 agcagctgct catccccgag atcttgcacg tgacgccggc gcaagccgag gcgggggagt
     9001 cgcacgcgca ggagtttgcc gcgctgggtt tcggcgttac gcgtctcgcg cccgaccagc
     9061 tggcgttgcg gtccattccg agtctgttgg cgggccgcga tccggcccgc atcgtgcgcg
     9121 atgtgctctc ggatctgctc gagcaaggtc attcgcggcg ggtcgaggag tccatcaatc
     9181 acttgctggc gaccatggcc tgccacgccg cggtccgcgc acagcgcaac ctgactctgc
     9241 ccgagatgaa tgcgttgctg cgggaaatgg aggttacgga ccgcgccgac cagtgcaacc
     9301 acggacgtcc gacctgggtg cggctgtcgc tcacggatct ggaccgcctg ttcttgcgtg
     9361 gccgatgatg ggggcaacga gcggccggcg cgcgatactc ctgatgggcc cgaccggctc
     9421 cggcaaatcc gacttggcgg tgcagcttgc cgccgcgctg cccgtggaaa tcgtcagcgt
     9481 ggattcggcg ctggtctatc ggggcatgga catcggtacc gccaagccga gcgcggcgtt
     9541 gcgcgcgcgc gtaccgcacc atctcatcga catccgcgat ccggtcgagc actactccgc
     9601 cggcgacttc actcgggatg ccgtcgccag catgcaggcc atctgggcgc gtggacgcca
     9661 cccgttgctg acgggcggca ccatgctgta tttccacgcc ttgagccatg gaattgccaa
     9721 gctgcccgcg gcggatccta agttgcgcgc cgagatcgac gcgcaggcgg cgtccggagg
     9781 gtgggcggcg ctgcatcggg aacttgccga agtcgacccc ggcgccgcat cgcgtatcca
     9841 tgtcaacgat ccgcagcgga ttcagcgtgc gctcgaggtc tatcggttga ccggcgagac
     9901 catcacgaaa ttgcaacaaa accgcgtgtc ggtgtttgcc gacgttgctg tgacggaaat
     9961 tgccgtggcc ccccttgaac gcagcgattt acacaccaga atcgaattgc gcttcgatgc
    10021 catgctcgcg gctggattgc tggcggaggt gcgcaggttt ttcgaaagaa gcgatttgac
    10081 cgcggaacat ccatcgatgc gcgcggtcgg atatcgacag atctggcggt atttagcggg
    10141 acactgtgct ttgagcgagg cgagagatca agcgattgcg gcaacgcgac aacttgccaa
    10201 gcgccagttg acgtggctgc gccggcgtga gcacgcgcag tggttcgact cgatgcattc
    10261 tgacgttgca cgcgcactga tgaatgcact gtctcagggc ggatttgcgc tgtcgagcgg
    10321 agtaacacca caaggtgtgc tgtgctagca tgaactcgaa tatgcctggt gccgcaagga
    10381 agtggttcgg caatccaaat gacactaaaa aacaaggagc tctacccatg gcccgtggcc
    10441 agtcactgca ggatccattt ctgaatgccc tgcgcaaaga acgagtgccc gtctcaattt
    10501 atcttgtgaa cggcatcaag ttacagggac agatcgactc tttcgaccag ttcgtcgtct
    10561 tgctcagaaa cagcgtgagt cagatggtgt acaaacatgc gatttccacg gtcgtgccgg
    10621 ctcgcaatgt gcgtttgccg accggcgagg atggagaagt gacggaaccg gcgatcgtcg
    10681 agtgatctcg atcgcggcgc ttcggccgat ggcgatgtct tcagtgactg ggcggcgatc
    10741 tgcaactgtt tgaacgcccg cgaggtggcg aacgcgccgt cctggtgggc gtcggcatcg
    10801 ggcacccggt cgatccgaac gatacggccg agttcgccgc tctcgcggca tccgccggtg
    10861 caatcgcggt ggcaacggtg ctcgcgtcac gtgcgcgtcc cgaccctaaa tacttcgtgg
    10921 gcagcggcaa agccgaggag attcgcagct gcgcccaatt gaatgcggcc gatgtggtgc
    10981 tggtggatca aaccttgagc cccagccagg agcgcaacct cgaaaaactc accggttgtc
    11041 gagtattaga tcgtaacggt ctgattttgg atatttttgc gcagcgagct cgcagtttcg
    11101 aaggcaaact gcaagtcgaa ctcgcgcagc tctcgcactt ggccacacgt ctggttcgag
    11161 gctggacgca tttggagcgg cagaaaggcg gcatcggtct gcgcggtccg ggcgaaaccc
    11221 aattggagac ggaccggcgc ctgatcgcca agcgcattcg aaccctcaag gcgcgcctcg
    11281 aaaagcttgg gcggcaacgc gataccagcc gcctcgtgcg ccgtgaagtc ccggtgccca
    11341 cggtcgccct ggtgggttat accaatgcgg gcaagtcgac gctgttcaac gcgctgacgg
    11401 gcgcagaaac ctacgtcgcc gatcagctgt ttgccaccct cgacccgact gtacgccgcg
    11461 tgaaattgcc cggaattggc gaggtggtat tggccgacac ggtcggcttc gtccgcgctc
    11521 tgccgcacga attggtggcc gctttcc
//
LOCUS       DASWZT010000369         6141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_41813, whole genome shotgun sequence.
ACCESSION   DASWZT010000369 DASWZT010000000
VERSION     DASWZT010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41813"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VGE92_10460"
     CDS             complement(<1..1008)
                     /locus_tag="VGE92_10460"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1314291.1"
                     /translation="MKANPKLACAIGAILSGVGGGMARTAIADEADTTDQIQEITVTA
                     QRRTENMQNVPVTIQALTAETLTQLNVQTFDDFVKYLPNVTATNTGPGQSSVFMRGLS
                     TTPQGVQGSGSVGGFPSVAIYLDDQSGALPGRNLDIYAADLERIEVLEGPQGTIFGAG
                     AESGVMRYITNKPKLDVTEGNVTAGYATTAHGDPSSNAEAVINLPLIADTLAVRAVVY
                     TDSEGGYINNVPATFSRQPSDYSIQYYYGGVVPSGPHSVTSINNNSLVGSGINTVNYQ
                     GIRVGLAYKINDQWDALLTQSYQDMKSNGVFYEMPHSSSVLPSLQQPLPDLSVESFVP
                     SF"
     gene            complement(1091..3130)
                     /locus_tag="VGE92_10465"
     CDS             complement(1091..3130)
                     /locus_tag="VGE92_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY1314292.1"
                     /translation="MLRRSPNLDIPPAVEMEVLRIRGLMEQRQFATALAAAEALAGDN
                     AQNRDALYMMAVCQRYLGRVPDALATLAQLEKWHPTFSRLFQERGYCHVARREAEPAI
                     QAFVRAVSLNHALAASWKALQTLFRMTNQAESAANADSHVAKLAELPAQIVTATGMLE
                     DGELEPAEKIVREYLLKHGNDVEAMRVLAKIGLELDVLDDAELLLESVLKLAPDYGAA
                     RYDYARVLLERHKHARARAELDKLLENEPDNRAYLMSYAAACAGLGEHERAMLLYRRL
                     LVETPQAEELHLSIGHAQKTLGHTCEAIESYRAARCRADFGDAYWSLANLKTYRFSDE
                     EMERMRGAESCETTPLEDRYHFCFALGKALEDRGDYAASFDYYRRGNALRKSLSRYRP
                     ETIERNARLQMQVCTREFFAERAGVGCPSDAPIFIVGLPRAGSTLLEQILASHSRVEG
                     TLELPDIPRLAAELVGRDGETDDPRYPAILSRMQPQEFRRLGEAYLADTRPYRTGKPH
                     FIDKMPNNFRHIGLIHLILPNARIIDARREPLACCFSNFKQLFASGQEFTYGLEDIAR
                     YYLNYVALMDHWDAVLPGKVLKIQHEELVEDLPGHVQRLLAFCGLGFEPACLEFHKTD
                     RSIRTASSEQVRQPINRLGLDQWRHFEPWLDPLKRQLGTLLHPHDASMRDRSNVL"
     gene            complement(3227..4837)
                     /locus_tag="VGE92_10470"
     CDS             complement(3227..4837)
                     /locus_tag="VGE92_10470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1314293.1"
                     /translation="MRWMSPLPAHGEPRFERFVMLCLTLGYPLFYALGFLSWSRTGSA
                     AIWPSHALTFAAFMALPVRRWALVAAAVLLWDTLSRPILLWGSDRAVASWAVTAGFAC
                     ANILTTAGPASLARAMRLFRFDERFRLVISPLWIVALFAGSVPGALLGCATSALDKGV
                     PLMPGELGLWVLAAVLSIVTFGPMVFGLLLGYPETPPTPARAWEGWALACMVLALFVC
                     FAAVPLRAVDPVLEPMWFAIPLVWLALRFSRRTTDIGVVIVASAVVFFAGHGVGIYRG
                     LANNGWRDVVISIDAFLVIGCGGALLVNLMTLRQRVLLDELAKEHLALRDYARALGVA
                     EDTARRKTAADLHDGIGQILAGQSMTLAAMRAHANDTPFSELLDEATEASREAQEGLR
                     VMIQDLSPPGLDQASLKETLRWLADFFETRFGFDVDWHVIGSVDPPRDRIQLIYRCTR
                     ELLMNARKHSQCQGAEVEVEVSAAAIEIAVIDEGVGFDPRRADPSPGKRFGLAQLRER
                     VRAAGGTLAVDTVIGEGCRVTVRLPSEP"
     gene            complement(4857..6053)
                     /locus_tag="VGE92_10475"
     CDS             complement(4857..6053)
                     /locus_tag="VGE92_10475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY1314294.1"
                     /translation="MTKHDTATQLQRELQLNEVQARHSAAVRDHLAQQIAASGGWLSF
                     ERFMESALYAPGLGYYSAGARKLGAGGDFTTAPEVSRLFGGCIARQCAEVLNALGGGS
                     ILEIGAGSGRLAADILLRLESLGELPERYWILDISADLRERQCRHLASHAPHLQDRVQ
                     WLDRPPELAFDGVVLANEVLDALPVARFSWRRSHVEELGVAIDDDGFRWESRPATATM
                     AHDCGQLANAARDWQDGYVSEYCPRLSAWTQRVTSALRAGAALWFDYGLPRSQYYLAE
                     RHEGTLMCHFRHRAHGDPFLYPGLQDITAWVDYTRLAEACRAAGFALAGFTTQSWFLA
                     ALDVDQEMQILAGESAGQLARLANEARQLMLPGEMGERFKAMAWTRGTQPLLSGFAQR
                     DLRHTL"
BASE COUNT         1149 a         1993 c         1909 g         1090 t
ORIGIN      
        1 gaaggacggt acgaagctct ccaccgacaa gtcgggcagc ggctgctgca ggctcggcag
       61 tacgctcgag ctatgcggca tttcatagaa cacgccgttc gacttcatgt cctggtacga
      121 ctgggtcagc aatgcatccc attggtcgtt gatcttgtac gcgagtccca cgcggatgcc
      181 ttggtagttg actgtattga tcccgctgcc gaccagggag ttgttgttga tcgacgtcac
      241 ggagtgcggg ccgctcggca cgacaccgcc gtaataatat tggatgctgt agtcgctcgg
      301 ctggcgactg aacgtcgcgg ggacattgtt gatgtagccg ccctcactat cggtgtaaac
      361 cacggcgcgc accgccaaag tatcggcaat caacggcaga ttgatcacag cctctgcatt
      421 gctgctggga tcaccatgcg ccgtcgtcgc gtagcccgcc gtcacgttgc cttcggtgac
      481 gtcgagtttg ggcttgttgg tgatgtagcg catcaccccg gattcggcgc cggcaccgaa
      541 tatcgtgccc tgcgggcctt cgaggacttc gatgcgctcc aagtccgcgg catagatatc
      601 gaggttgcgg cccggcagcg cgccggattg atcgtcgagg tagatcgcga cgctcgggaa
      661 gcccccgacg ctgcctgatc cctgcacacc ttgcggagtc gttgataaac cgcgcatgaa
      721 gaccgaactt tggccgggac cggtgttggt tgcggtgacg ttgggcaggt acttgacgaa
      781 atcatcgaac gtctgcacgt tcaattgagt cagtgtttcc gcagtcagcg cctggatcgt
      841 gaccggcacg ttctgcatgt tctccgtgcg acgctgcgcc gtcacggtga tttcctggat
      901 ttggtccgtc gtatcggcct cgtcagctat cgctgtccgg gccatgccgc cgccgacgcc
      961 actcaggatt gcgcctatcg cgcaagccag tttcggattc gccttcatgt gtccccatcc
     1021 ctactattat tgatcgcgcg gctgtcgccg cggagctgat gacgccggca ctccggggtg
     1081 ctgcttgcga tcataagaca ttcgagcgat cccgcatact cgcgtcgtgc ggatgcaaca
     1141 aggtacctag ctgccttttc aacgggtcga gccacggttc gaagtggcgc cattggtcga
     1201 gccccaggcg attgatcggc tgtcgcacct gttccgagct cgccgtgcgg atgctgcggt
     1261 cggtcttgtg gaactcgagg caggcaggct cgaacccaag gccgcagaac gcgagcaggc
     1321 gctgcacatg gcccggcagg tcctcgacca gctcttcgtg ctggatcttg aggaccttgc
     1381 cgggcagcac ggcgtcccaa tgatccatca gggcgacata gttcaagtaa tagcgcgcga
     1441 tgtcctcgag accgtaggtg aattcctggc ccgaggcgaa taactgcttg aagttactga
     1501 agcagcaggc gagtggctcg cgccgggcat cgatgatgcg cgcattgggc aaaatcaagt
     1561 ggatgagccc gatatgccgg aagttattcg gcattttatc gatgaaatgt ggtttgccgg
     1621 tacggtaggg acgggtatcg gccaggtagg cctccccgag gcgccggaac tcctgcggct
     1681 gcatgcggct gagaattgcc ggatagcgcg gatcgtcagt ctcgccgtcg cggcccacca
     1741 gttccgcggc cagacgcgga atgtccggca gttcgagcgt gccctcgacg cgcgaatgcg
     1801 aggcaaggat ttgttccagc agcgtcgatc cggcgcgggg cagtccgacg atgaatatcg
     1861 gcgcatcact cgggcacccg accccggcac gctcggcgaa gaactcccgc gtgcaaacct
     1921 gcatctgcag ccgcgcattt cgctcgattg tctccggacg ataccggctc agcgattttc
     1981 tcagtgcgtt gccccggcgg tagtagtcga acgaggcggc gtagtcgccc cggtcctcca
     2041 gtgccttgcc gagcgcgaag caaaaatgat aacgatcctc gagcggcgtg gtttcgcacg
     2101 actccgcgcc gcgcatgcgc tccatctcct cgtcgctgaa gcgataggtc ttgaggttcg
     2161 caagactcca atacgcgtcg ccgaaatcgg cccggcaacg cgcggcgcgg tacgactcga
     2221 tcgcttcgca ggtgtggccg agcgtttttt gcgcatgacc gatcgacagg tgcaattcct
     2281 ccgcctgggg agtttcaaca agcagtctgc gatacagcag catggcgcgt tcatgttcgc
     2341 cgagaccggc gcaagccgcc gcataggaca taaggtacgc ccggttgtcg ggctcatttt
     2401 ccaatagctt gtcgagctcg gcacgggccc gcgcatgctt gtggcgttcc agcaacaccc
     2461 gggcatagtc atagcgtgct gccccgtagt ccggcgccag cttgagcacg ctttcgagca
     2521 gcagctccgc atcgtccagc acatcgagtt ccaggccgat cttggcgagc acgcgcatgg
     2581 cctcgacgtc attgccatgc ttcaggaggt attcgcggac gatcttctcg gccggctcaa
     2641 gctccccgtc ctcgagcatt ccggtcgcag tgacaatttg cgcaggcagc tccgccagtt
     2701 tggcgacgtg actatcggcg ttggcagcgc tctctgcctg attggtcatg cgaaacagcg
     2761 tctgcagggc cttccagctg gccgcgagcg cgtggtttag gctcacggca cgcacgaatg
     2821 cctggatcgc cggttcagcc tcacgacgcg ccacgtggca gtagccgcgc tcctggaaca
     2881 ggcggctgaa cgtcggatgc cacttctcga gttgcgccag cgtcgcaagt gcatcgggga
     2941 cgcggcccag ataacgctgg cacacggcca tcatgtacag ggcgtcgcgg ttttgcgcat
     3001 tgtccccggc cagcgcctcg gctgcggcca gtgccgtcgc gaactggcgt tgctccatca
     3061 ggccccgaat gcgcaggacc tccatctcca ccgcaggagg aatatcgagg tttggactac
     3121 gcctaagcat ggagtgtgca aatcatccgc cgctcatcct acagtgaaag ccggcgagtt
     3181 cgaaaccgcg gcgcgagact tgcgcatcgc ggacatgctt gcgaggtcag ggttccgatg
     3241 gcagtcgcac cgtgacgcgg caaccctcgc cgatgaccgt gtcgacggcg agagtacccc
     3301 ccgctgcgcg gactcgctcg cgcagctgtg cgaggccgaa tcgcttgccg ggcgaggggt
     3361 ccgcgcggcg cggatcgaag cctacaccct cgtcgatgac ggcaatttcg atcgccgccg
     3421 cagagacttc gacctcgact tcagcgccct gacactgcga gtgcttgcgc gcgttcatca
     3481 gcagttcgcg ggtgcatcgg tagatgagct ggatgcggtc gcgcggcgga tccacgctgc
     3541 cgatcacatg ccagtcgacg tcgaatccga atcgcgtttc gaaaaagtcc gcgagccagc
     3601 gcagcgtctc tttgagcgag gcctgatcga gacccggggg gcttaggtct tgaatcatta
     3661 cgcgcaggcc ctcctgagcc tcgcgcgagg cctcggttgc ctcgtcgagc agctccgaga
     3721 acggcgtatc gttcgcgtgc gcgcgcatcg ccgcgagagt catgctctgc ccggccagaa
     3781 tctgcccgat tccgtcgtgc aaatcggccg ccgtcttgcg gcgtgcggtg tcctccgcga
     3841 cgccgagggc acgcgcatag tcgcgcagtg ccaggtgttc ctttgccagc tcatcgagca
     3901 gcacacgctg cctcaaggtc atcaaattga ccaacagggc gccgccgcag ccgatgacga
     3961 ggaacgcgtc gatcgaaatg acgacatccc gccaaccgtt gttggcaagc cctcgataga
     4021 taccgacgcc atgaccggcg aagaatacga cagccgacgc cacgatcacc acgccgatgt
     4081 cggtcgtgcg ccgcgaaaat cgcagcgcca accagaccag cgggatggca aaccacatcg
     4141 gctcgagcac gggatcgacc gcacgcaagg gcactgcggc aaagcagacg aacagcgcaa
     4201 gcaccatgca cgccagggcc catccctccc aggcgcgcgc cggtgtcggc ggcgtctcgg
     4261 gatatcccag gagcagcccg aacaccatcg gtccgaatgt gacgatcgac aagaccgctg
     4321 ccaggaccca caaccccagc tcgccgggca tcaggggaac gcccttgtcg agtgcactgg
     4381 tggcgcagcc gagcaaggcc cccggcacgg aaccggcgaa cagcgccacg atccataagg
     4441 gcgagatcac cagccgaaaa cgctcatcga agcgaaacag ccgcatggca cgggccaggc
     4501 tcgcgggacc cgcggtcgtc agaatgttgg cgcatgcaaa gcccgcagtc acggcccacg
     4561 aagcgaccgc tcgatcgcta ccccacagca gaatcggcct cgacagcgtg tcccacaaca
     4621 gcacagccgc ggccaccagc gcccagcggc gaaccggcag cgccatgaat gcggcaaatg
     4681 tgagcgcgtg ggagggccag atggcggccg aaccggtacg cgaccacgat agaaatccga
     4741 gtgcgtagaa cagcgggtat ccgagagtca agcacagcat cacgaagcgc tcgaaacggg
     4801 gttcgccgtg ggccggcagc ggactcatcc acctcatcgc aagcccttga ttgccatcat
     4861 aaggtgtgcc gcagatcgcg ctgcgcaaag ccggatagca ggggctgcgt gccgcgcgtc
     4921 catgccatcg ccttgaaccg ctcgcccatc tcgcccggca acatcagttg ccgcgcctcg
     4981 ttcgccaaac gtgcaagctg gccggcgctc tcgcccgcga ggatttgcat ctcttgatcc
     5041 acgtcaagcg ccgccaggaa ccagctctgt gtcgtgaagc ctgccagcgc gaagcccgcc
     5101 gcgcggcaag cctcggcgag ccgcgtgtag tccacccacg ccgtgatgtc ctgcagccct
     5161 gggtatagaa acggatcgcc gtgcgcccga tgacggaagt ggcacatcaa ggtgccctcg
     5221 tgccgctctg ccaaatagta ctgggatcgg ggtagaccat aatcgaacca cagcgccgcc
     5281 ccggcgcgta acgctgaagt gacgcgctgc gtccacgcgc tcaggcgcgg acagtattcc
     5341 gacacgtaac cgtcctgcca atcgcgcgcg gcgttcgcga gctgcccaca atcatgcgcc
     5401 atggtggccg tcgccggccg cgattcccac cggaacccgt catcgtcgat ggcgacgccc
     5461 agctcttcca cgtggctgcg acgccaactg aaccgcgcca ccggcagtgc gtccagcact
     5521 tcattggcaa gcacgacccc gtcgaatgcc agttccgggg gtcgatcgag ccactgcacg
     5581 cgatcttgca aatgcggcgc atgcgaggcg aggtgccgac actgccgttc gcgcaggtcc
     5641 gcgctgatgt cgagaatcca ataacgctcg ggtaattcgc cgagcgactc gaggcgcagc
     5701 aaaatatccg cggcgagacg accgctgccg gcacctattt cgaggatgga gccgcccccg
     5761 agcgcgttca agacctcggc gcattgtcga gcgatgcagc cgccgaacaa gcgcgagact
     5821 tccggtgcgg tggtgaaatc gccgcctgcg cccaacttgc gcgcgcctgc gctgtagtac
     5881 cccaagccgg gcgcgtacag cgccgattcc atgaatcgct cgaaggagag ccaaccgccg
     5941 gacgcggcaa tctgctgcgc caaatggtct cgcacggccg ccgaatgccg cgcttgcacc
     6001 tcgtttagct ggagttcacg ctgcagttgc gttgcggtat cgtgcttggt catggcatcc
     6061 agtatcgaga attcgcgatt gcgagaccag gtcgtgctca ttaccggcgg cgcgcgccgc
     6121 gtcggcgccg agatcgcgcg c
//
LOCUS       DASWZT010000370         1934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_7686, whole genome shotgun sequence.
ACCESSION   DASWZT010000370 DASWZT010000000
VERSION     DASWZT010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7686"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="VGE92_10480"
     CDS             <1..1023
                     /locus_tag="VGE92_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314295.1"
                     /translation="VLDVRNPSKGMVIHHAIDGSRRVMVQNADRSRAYIPAKGIPYVQ
                     HPYTFRGQQLDHRTFVVQGQLFHQIYRPYTYNGTNLDVYATSRYYAPKFYNWVGNKVA
                     TPQKFNWPYNNSTPWYGHYQGFFTPESSYTSPAQWLTDYMMAATLYVAYTTKGQASDP
                     PPASSAPVTPEVKQMLTEEVSRQVKQESVEAADNAQNRAPQPGAGSVVQELSDHESHA
                     FVVSSDLDLVDPSGRRCALSEADVVQVTSGPNADTGAVDAVVLVSKGGVECTKATQVE
                     IALNDVQEMQNHMRAVIDQGMATTPAANNSQPVTPAFAAAAPPADQSATQEIEQQTQI
                     AAAADG"
     gene            1138..>1934
                     /locus_tag="VGE92_10485"
     CDS             1138..>1934
                     /locus_tag="VGE92_10485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEY1314296.1"
                     /translation="MASTARPIASVTISFGLVAIPVKLYSATVPSERISFHLLRKSDG
                     SRIKEQFVAVNDGKRVDRSDLAKGYEFSKGKHVMFTTEELKLLEDSGSRELAIAQFVP
                     LESVDPVYFVATYYLLPDKGSAKPYALLATALRQEQRCAVGKWTSRGQEHVIIIRPIE
                     DGLALHQLHFKAEVRQLKDFDPHRTPVSDAEVKLARQLIEQLSVPRFDPGEFVDEHRA
                     RVQALIRKKVQGKEISIAEPEVEKRGGNVIDLMDALKASLQRRGAAA"
BASE COUNT          429 a          591 c          573 g          341 t
ORIGIN      
        1 gtcctggatg tgcgaaatcc cagtaaaggc atggtgattc atcatgcgat cgacggcagc
       61 cgccgggtca tggttcagaa cgcggaccgt tcgcgggcct acatacccgc aaagggcatc
      121 ccgtatgtgc agcatccata taccttccgc ggccagcagt tggaccaccg caccttcgtg
      181 gttcagggac agctatttca ccagatctac cgcccttaca cctataacgg cacgaacctc
      241 gacgtctacg cgacctcgcg ctactacgcg ccgaaatttt ataattgggt cggcaacaaa
      301 gtcgccacgc cgcagaaatt caactggccg tacaacaact cgaccccgtg gtacggccat
      361 taccagggct tctttacgcc ggaatcatcg tacacatcgc cggcccagtg gctgaccgac
      421 tacatgatgg ccgcgacgct gtacgttgcc tatacaacca aaggacaagc gtcggatcca
      481 ccgcccgcga gcagcgcgcc ggtgacaccc gaagtcaaac agatgctcac cgaggaggtc
      541 tcacgtcagg tcaagcagga gtcggtcgaa gcggccgaca acgcgcagaa tcgcgcgccg
      601 cagccgggcg cgggcagcgt cgtccaagaa ctttcggatc atgaatccca cgcgttcgtc
      661 gtatcttccg acctcgatct ggtcgatccg tcgggacggc gctgcgcgct gagcgaggcg
      721 gatgtggtgc aagtcacctc gggtccgaat gcggacacgg gcgcggtcga cgccgtggtc
      781 ctcgtcagca agggcggcgt cgagtgtaca aaggccaccc aggtcgagat tgccctcaac
      841 gacgtccagg agatgcaaaa tcacatgcgt gcggtcatcg atcagggaat ggcgaccacg
      901 ccggcggcga acaattcgca gcctgtgacc ccggcgttcg cggccgcggc acctcccgca
      961 gatcagagcg cgacgcaaga gatcgaacag cagacgcaga ttgcggcggc cgccgacggc
     1021 tgaaggcggc gcgccctcgg atcagtctta cacacagaca cgctcgcgcg aaccgcacgt
     1081 gcgctcatga atctgtaaca tatcagtcga gagtcccgat cgtcatcaga ggttatcatg
     1141 gcttcaacgg cgcgccccat cgccagcgtt acgatttcat tcgggttggt cgcgatcccg
     1201 gtgaaactgt actcggcgac cgtcccctcc gaacgcattt cctttcatct gctgcgaaaa
     1261 tccgacggct cgcggatcaa agagcaattt gttgcggtga atgacggcaa gcgcgtcgat
     1321 cgatccgact tagccaaagg ctacgaattc tcgaaaggca agcacgtaat gttcacaacc
     1381 gaagaactca aactgctcga agattcgggt tcccgggagt tggcgatcgc gcaattcgtg
     1441 ccgctggaat cggtcgatcc cgtgtacttc gtggcgacct attatctctt gccggacaag
     1501 ggctcagcga agccgtacgc attgctcgcc acggcgctgc ggcaggagca gcgctgtgcg
     1561 gtgggcaagt ggacctcgcg gggccaagag cacgtcatca tcatcaggcc gatcgaagac
     1621 gggctcgctc tgcatcagct gcatttcaag gccgaagtgc gccagctgaa ggatttcgat
     1681 cctcacagaa caccagtgtc cgacgcggaa gtcaaactcg cgcggcaact gattgaacag
     1741 ttgtccgtcc cgcgatttga tcccggcgaa tttgtggacg agcatcgcgc gcgagtgcaa
     1801 gcactgatca ggaagaaagt ccagggtaag gagatctcta tcgcggagcc cgaggtcgag
     1861 aaacggggcg gcaacgttat cgacttgatg gatgcgctca aggccagcct gcagcggcgc
     1921 ggagcggcgg cgaa
//
LOCUS       DASWZT010000371         5322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_46574, whole genome shotgun sequence.
ACCESSION   DASWZT010000371 DASWZT010000000
VERSION     DASWZT010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46574"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VGE92_10490"
     CDS             complement(<1..823)
                     /locus_tag="VGE92_10490"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEY1314297.1"
                     /translation="MSARYRRTPRRCLQLALLGLAVGAGALPRAAWSQTPSPLQEWQY
                     SGGIVLAKLFQPNLPQWSVITGVAAETQPIYDGAQAYRVQGGPVINIQYYDIAFLTTG
                     EGLGINFLRGDHYRLGAAISYDLGRRVRDDYANLQGMGNIEAAPVAKLFGSYVLSKKF
                     PLILRVDARQFVGGANGAIGDVGLYMPLPGSSKKFVMFAGPSVTFATHRYLQSEFGVS
                     QAQSLASGHPVFDPNPGMVSVGVGFSATEFITDHWFLNIDAALTKLKGSPDISPLT"
     gene            1274..2050
                     /locus_tag="VGE92_10495"
     CDS             1274..2050
                     /locus_tag="VGE92_10495"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEY1314298.1"
                     /translation="MLLALSTWPEIDVWLRRSRSVVIPIGSNEQHGPTGLLGTDWLCP
                     EIIAHEAHRGGGDILVAPTFNIGMAQHHLGFAGTISLRPSTFQAAICDWVQSLSRHGF
                     ERIYFLNGHGGNIASIEAAFSEAYAHFSFGARICPYMLKLRSWWQLPGMGSLADELFP
                     TGHGTHATPSEIAITQSAYPDSIKNAVCTPQLAPAGPVRDALDFRGRFADGRMGSDPL
                     QASPEKGARLIASGAEALLKDLAEFSREPFVVKPPSEPGP"
     gene            2047..2691
                     /locus_tag="VGE92_10500"
     CDS             2047..2691
                     /locus_tag="VGE92_10500"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEY1314299.1"
                     /translation="MKVSWVFDVISPFAYLGLKQMHELPARVELEYKPVLLAGLLAHH
                     GQLGPAEMPTKRRFTYRFALWRSRRTGIKMQMPPAHPFNPLAALRLIIAAGCSFPAVE
                     LVFDAIFQHGLDATDPKVIERLGVELGIKDPMAAINDATVKEVLRQNTDWAIAKGIFG
                     VPTFLVGEEIFWGHDAFEMLLGYLRDPAAFDDQQMRALDSLPIGARRSEPPRQS"
     gene            complement(2651..3706)
                     /locus_tag="VGE92_10505"
     CDS             complement(2651..3706)
                     /locus_tag="VGE92_10505"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HEY1314300.1"
                     /translation="MKSELVLVTGGSGFIGAHCIVQLLHAGYRVRTSVRSLARESEVR
                     AMLKEGGAEPGHGLSFVAADLNSDAGWPEAVAGCAFVLHVASPFPATAPAHENELIIP
                     AREGTLRVLRAARDARVARVVVTSSFAAIGYGTLPVRPFTEEDWSELSGNVSAYVKSK
                     TLAERAAWEFIAREGKDLQLAVINPVVVFGPVLGADNSTSTQLVKRLLEGMPGVPRLT
                     FGVVDVRDLADLHLRAMTSQAARGERFIGAAGRFLSIQEIAQVLRARLGEAAQRVPTR
                     VLPDWLVRLAALRDSGVKQMLPELGKVKSASSDKARRVLGWAPRSNEEAIVATAESLL
                     RLGLLKTGAVAPSAARR"
     gene            complement(3703..3969)
                     /locus_tag="VGE92_10510"
     CDS             complement(3703..3969)
                     /locus_tag="VGE92_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DksA/TraR family C4-type zinc finger protein"
                     /protein_id="HEY1314301.1"
                     /translation="MATGWAKEGAVQEQIDATVKDAVKRAQSSLPQGASLKNCAECAA
                     EIPQARRAAIAGVRLCLSCQETHDQDAARFSGYNRRGSKDSQLR"
     gene            complement(4041..>5322)
                     /locus_tag="VGE92_10515"
     CDS             complement(4041..>5322)
                     /locus_tag="VGE92_10515"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY1314302.1"
                     /translation="WTACGLIVAWVGCFTLFPALQSLLKTPLRSEQVPVAKWFPGFVD
                     KLVPATRRYRWPMVGGALILMGCGAIALFGFPNVLAPLALETDVLTYVNPSERVAQDT
                     RHFQESNALDAVELWLQTPPAQALDPEFLRAVELLTEQLERDPRITSVDGPTSVLRWA
                     RYVQTGSDALPAAAAAWPKLAADLEQILLTAPSARQYVDVKDLANVRLSIRGRAKLFG
                     RTGAMRSFVEHQWAAAQAAQPALRAVRGSVVGKGVLGTEITQRLLPTLTQSFALTASV
                     IFMAFLLVFRSPSARLMTMIPSLFAILCVFLVMRLAAISLNIATILIGSTVLGATEND
                     QIHFFYHFQEGHSTGTTAGALQHAMLVAGRPILFATLINASGFLALALSDLPPMREFG
                     IVSASAFVLALLADFTALPGALWILARDDRPRAA"
BASE COUNT          997 a         1695 c         1687 g          943 t
ORIGIN      
        1 cggtcagcgg gctgatatcc gggctgcctt tcagcttggt gagcgcggca tcgatattca
       61 gaaaccagtg gtcggtgatg aattccgtgg cgctgaaacc cacgccgacg gagaccatgc
      121 cggggttggg gtcgaacacc ggatggccgg aggcgagcga ctgggcctgg ctcaccccga
      181 attcgctctg caaatagcgg tgcgtcgcaa acgtgaccga tgggccggca aacatgacga
      241 attttttcga actgccgggg agcggcatat acaggcccac atcgccgatg gcgccgttgg
      301 ctccgccgac gaactgtcgt gcatctaccc gcagaatcag cgggaatttt ttcgacagca
      361 cgtaggagcc gaataattta gccacgggcg ccgcctcgat gttgcccatg ccttgcagat
      421 tggcatagtc gtcccggaca cggcggccca agtcgtatga gatggcggct cccagccggt
      481 aatgatcgcc gcgcaggaag ttgatgccga gaccctcgcc ggtggtgagg aaggcgatgt
      541 catagtactg aatgttgatg accgggccgc cctgcacgcg gtacgcctgc gcgccgtcgt
      601 aaatcggttg ggtttccgcc gcgacgccgg taatcacgct ccattgcggc aggttcggct
      661 ggaacaactt ggcgaggacg atgcctcccg aatactgcca ctcttgcagt ggcgagggcg
      721 tctgcgacca cgcggcacgc ggcagggcgc cggcccctac agccaagcca agcagcgcca
      781 gctgcaggca tcttctcggc gttcgacgat atcgagcgga cattttgcat tctctccacg
      841 ctgctagcgc ctgtgttgcg aagctatagc agaaattcca tgaattcagg agagctgcaa
      901 atcccgacgg tcgttatcgg actaattgct gctcacaggt cttgacggcg ccttgcgttt
      961 tggcgggggt ttcagcgacg ctttgaccgc atgattgatg gtcggtgccg tcaaaatttg
     1021 ccgttcagcc tcgagccaat cgtcatctgc agagccatgc gggcggccgc gagcctccca
     1081 aagtcggtac gccaggtccc gaatggcgcg atattcatcc tgcataaggg cctccgaagt
     1141 catgcacagc aacttaaggc gtaggccggc gccggggtag gacggcgcgg cggcaatttc
     1201 ggtcagccaa gtcctacaga aaacccttaa attccgaccg cgggttgcgg tagggtatgc
     1261 cccgaggtgg cctatgcttc ttgccctatc cacgtggcct gaaatcgacg tctggctgcg
     1321 gcgctcacgt agcgtcgtga ttcccatcgg gtcgaacgaa cagcacggac cgaccggcct
     1381 gctcgggacc gactggctgt gcccggagat cattgcgcat gaagcgcacc gcggcggcgg
     1441 cgatattttg gtcgcgccga ccttcaatat cggcatggcg caacaccact tgggtttcgc
     1501 gggcacgatc tcgctgcgtc cgtcgacctt ccaggcggcg atctgcgatt gggtgcagtc
     1561 cttgagccgg cacggcttcg agcgaattta tttcctcaac ggacacggcg gcaatatcgc
     1621 ctcgatcgaa gcggcattct ccgaagccta cgcacacttt tctttcggcg cacgcatctg
     1681 tccgtacatg ctcaagctgc ggagttggtg gcaactcccg ggcatgggca gcctcgccga
     1741 cgaattattc ccgaccgggc atggcacgca tgcgaccccg tcggaaatcg ccatcaccca
     1801 aagcgcctat cccgactcca taaaaaatgc cgtctgcacg ccgcaactgg cgcccgcggg
     1861 accggtgcgc gacgccttgg atttccgcgg acgtttcgcg gatggccgaa tggggtcgga
     1921 ccccttgcag gcctcgccgg agaagggtgc gcgcctgatc gcaagcggtg ccgaggcgct
     1981 gctcaaggat ctggcggagt tctcgcgtga gcccttcgtc gtcaaaccac cgagcgagcc
     2041 cgggccgtga aagtctcgtg ggtattcgat gtgatttcgc cgtttgccta cctcggcttg
     2101 aagcagatgc atgagttgcc ggccagggtc gagctcgagt acaaacccgt gttgctcgcc
     2161 gggttgctcg cgcatcacgg ccagttgggt cccgccgaaa tgccgaccaa gcgccgtttc
     2221 acgtatcgct tcgcgttgtg gcgcagccgg cgcacgggta tcaaaatgca gatgccgccc
     2281 gcgcacccgt tcaacccgtt ggcggcgctg cggctgatca tcgcggcggg ctgcagcttc
     2341 ccggccgtcg agctcgtgtt cgatgcgata ttccagcacg ggctcgacgc gaccgacccc
     2401 aaggtcatcg agcgcttggg cgtcgagctc gggatcaaag acccgatggc cgcgatcaac
     2461 gatgcgaccg tgaaggaggt gctgcgtcaa aacaccgact gggccattgc caagggtatt
     2521 ttcggcgtgc ccacgttctt ggtgggcgag gaaatctttt ggggtcacga tgcctttgaa
     2581 atgctgctcg ggtacctgcg tgacccggcc gcattcgacg atcaacagat gcgtgcgctc
     2641 gactccctgc ctatcggcgc gcggcgctcg gagccaccgc gccagtcttg agcaagccga
     2701 gccgcagcag gctctcggcg gtcgcgacga tcgcctcttc gttcgagcgc ggcgcccaac
     2761 cgaggacgcg tctggctttg tcgctgctgg cgcttttcac cttgccgagt tcgggcagca
     2821 tttgcttcac ccccgagtcg cggagcgccg caaggcgcac cagccagtcg ggcaaaaccc
     2881 gcgtcgggac gcgttgtgca gcctctccca gccgcgcccg caggacctgg gcgatttcct
     2941 gaatgctcag gaagcggccg gcggcgccta tgaaacgttc gccgcgcgcg gcttgcgaag
     3001 tcatggcgcg caggtgcagg tccgccaggt cgcgcacgtc gacgaccccg aaggtgaggc
     3061 gcgggacgcc cggcatgccc tcgagcaatc gcttcacgag ttgcgtggac gtggagttgt
     3121 ccgctccgag tacgggcccg aagaccacga ccggattgat gacggcgagc tgtagatcct
     3181 tgccctcgcg ggcgataaat tcccacgcgg cgcgctccgc cagagtcttg gacttgacgt
     3241 aagcgctgac attgccgctc aactccgacc agtcctcttc ggtgaagggc cgaacgggaa
     3301 gggtaccgta gccgatggcg gcgaacgatg aggtcacgac gacgcgcgcg acgcgggcgt
     3361 cccgagccgc gcgcaacacg cgcaacgtcc cctcgcgcgc aggaatgatc agttcattct
     3421 catgagcagg tgcggttgcg gggaacggcg aggcaacatg cagcacgaat gcgcagcccg
     3481 cgacggcctc cggccaacct gcgtcggagt tcagatcggc cgcaacgaag gacagcccgt
     3541 gtccgggctc ggcgccgcct tccttgagca tcgcgcgcac ctcgctttcg cgggcgagcg
     3601 aacgcacact ggtacgcacc cgatagcctg catgcaacag ctgcacgatg cagtgtgcgc
     3661 cgatgaagcc cgagccgccg gtcaccagca ccaactcgct tttcaccgca gctgactatc
     3721 cttgctgccg cgccggttat agccgctgaa gcgcgccgca tcttgatcgt gggtctcttg
     3781 acaggacagg cacaaccgca cgccggcgat ggcggcgcga cgcgcctgtg gaatctccgc
     3841 tgcgcactcg gcgcaattct tgagactcgc tccctggggc agactactct gcgcacgctt
     3901 gaccgcatcc ttgacggtcg cgtcgatctg ctcttgtacc gcgccttctt ttgcccaacc
     3961 tgtggccatg cccatctcct cgagcgatgc cgcccatccc tatcattaga gctgatcggc
     4021 caaattacaa gccaaagctg ctaggcagcg cgtgggcggt catcgcgggc gaggatccac
     4081 aaggcgcccg gcaaggcggt gaagtcggcc aacagcgcga ggacgaaggc ggacgccgag
     4141 acgatgccga attcgcgcat cggtggcagg tccgagagcg caagcgccaa aaagccgctc
     4201 gcgttgatga gcgtcgcgaa cagaatcggg cgtcccgcga ccagcatcgc atgctgcaac
     4261 gcgccggcgg tggtaccggt cgagtggcct tcctgaaagt ggtagaaaaa atgaatctgg
     4321 tcgttttcgg tggcgccgag caccgtcgag ccgatgagga tggtcgcgat gttgagcgaa
     4381 atagcagcca gccgcatcac caagaagacg cagaggatgg caaacagcga ggggatcatg
     4441 gtcatcaatc gcgccgaggg gctgcgaaac accaacagga atgccataaa aatgacactc
     4501 gccgtgagcg caaaactttg cgtcagcgtc ggcagcaggc gctgcgtgat ctccgtgccg
     4561 agcaccccct tgccgacgac gctgccgcgc accgcgcgca gtgccggctg cgctgcctgg
     4621 gcggccgccc attgatgctc gacgaagctg cgcatcgcgc cggtgcggcc gaagagtttc
     4681 gcacgaccgc ggatgctcaa gcgcacattg gcgaggtcct tgacgtcgac atactgacgc
     4741 gcgctcggcg ctgtcaataa aatctgctca agatcggcgg cgagtttagg ccacgccgcg
     4801 gccgccgccg gaagtgcatc ggatccggtt tgtacgtagc gcgcccagcg cagcaccgat
     4861 gtcggcccgt cgaccgaggt gatgcgtgga tcccgttcga gttgttcggt gagcaattcg
     4921 acagcgcgca gaaactccgg atcgagtgcc tgggcgggcg gggtctgcag ccacagttcg
     4981 accgcgtcga gcgcattcga ctcttgaaaa tgccgcgtgt cctgcgccac gcgctcgctc
     5041 ggattgacat aagtcaacac gtcggtctcg agcgcgagcg gcgccaacac gttcgggaaa
     5101 ccgaacagcg cgatcgcccc gcatcccatc aggatgagcg caccgcccac catcggccag
     5161 cggtaacgac gggtcgccgg caccagcttg tcgacaaatc cgggaaacca tttggccacc
     5221 ggcacctgct cggagcgcag cggcgtcttg agcagcgact gcagtgccgg aaacagagta
     5281 aagcatccga cccaggcaac gatgagtccg cacgctgtcc ac
//
LOCUS       DASWZT010000372         7205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_65768, whole genome shotgun sequence.
ACCESSION   DASWZT010000372 DASWZT010000000
VERSION     DASWZT010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7205
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65768"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /gene="hslV"
                     /locus_tag="VGE92_10520"
     CDS             <1..464
                     /gene="hslV"
                     /locus_tag="VGE92_10520"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEY1314303.1"
                     /translation="DGQVTLGQSVMKSNARKVRRLYGGKVLAGFAGGTADAFTLFERF
                     EAKLEKFGNLTRAAIELAKDWRSDRSLRRLEALLCVADTESTYVISGNGDVIEPEHDL
                     IAIGSGGGFAQAAATALMAGDLSAREIVERSLNIAADICIYTNRNLTIEEL"
     gene            471..1799
                     /gene="hslU"
                     /locus_tag="VGE92_10525"
     CDS             471..1799
                     /gene="hslU"
                     /locus_tag="VGE92_10525"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003740291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEY1314304.1"
                     /translation="MSQMTPREIVEELDKHIVGQKAAKRAVAIALRNRWRRSQVAEAL
                     RAEITPKNILMIGPTGVGKTEIARRLARLAKAPFIKVEATKFTEVGYVGREVDSIVRD
                     LVEISVKMTREEEIAKVRHRAADSAEERVLDVLLPRPAAGFTGEATPRDSETRQRFRK
                     MLREGHLEDREIEIDVRAAPMGVEIMAPPGMEEMTQQLQGMFQNLGAGRTRQRKLKIK
                     EALKLLVDEEAAKMINEEELKLRAVENAEQNGIVFIDEIDKIARRQETAGADVSREGV
                     QRDLLPLVEGCTVNTKYGTVKTDHVLFIASGAFHMSKPSDLIPELQGRLPIRVELEPL
                     VVEDFVRILTEPDASLCRQYTALLATEGLNLEFAASGVQRLAEVAFEVNERTENIGAR
                     RLHTVMERLLETVSFDAADRNGTHVLVNREYVEGNLGELVKDQDLSRYIL"
     gene            1809..2150
                     /locus_tag="VGE92_10530"
     CDS             1809..2150
                     /locus_tag="VGE92_10530"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEY1314305.1"
                     /translation="MPQPTEIKLRTQSRLLEVRFDDGSHFELPFEYLRVFSPSAEVTG
                     HGAGEGVLQTGKQNVGISGVEPVGNYALRLLFDDGHNTGLYTWTLLHELGREQRRNWA
                     RYLERCAESGR"
     gene            2198..2950
                     /gene="ubiE"
                     /locus_tag="VGE92_10535"
     CDS             2198..2950
                     /gene="ubiE"
                     /locus_tag="VGE92_10535"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008080626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEY1314306.1"
                     /translation="MDNSDRKVDFGFEKVAWGDKARRVHSVFASVAGKYDVMNDLMSL
                     GVHRLWKRFALSLTGLRPGQRALDVAGGTGDLAAGMLKQVGRSGHVVLSDVNPAMLAI
                     GRDRLLDQGWVANIDCIVADAERLPFDDNSFDCVTIGFGLRNVTDKAAALDSMRRVLK
                     PGGQLLVLEFSTPVAPGLKPIYDAYSFKVLPLLGRIVANDESSYRYLAESIRMHPDQE
                     TLLEMLRAAGFAQARYHNLTGGIVALHRGYKI"
     gene            2952..3599
                     /locus_tag="VGE92_10540"
     CDS             2952..3599
                     /locus_tag="VGE92_10540"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEY1314307.1"
                     /translation="MPATAAWLASAEALINRGIDASAQAGALARRLQGTVLQIDVDGI
                     THLRATACGARLALLTADAAAAGGAGTADAAISGSLPALLRLFNGGTNPASGRRAAPG
                     TPAQIRGDAEIANLYRELFLLARPDLEEELSRWIGDFAARRVAQVAARALAFARHARR
                     TAGDNIAEYLQEESRDLVNKTELEEFLRGVDTLRETADRIDARLASLERRLAGGA"
     gene            3599..5251
                     /gene="ubiB"
                     /locus_tag="VGE92_10545"
     CDS             3599..5251
                     /gene="ubiB"
                     /locus_tag="VGE92_10545"
                     /inference="COORDINATES: protein motif:HMM:NF003404.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEY1314308.1"
                     /translation="MRLRVLVRLLQIQRALVRHGLDDFVRATHLYRPFRFLVYLSPWT
                     WFQRSSGPRGERLRLALEELGPIFVKFGQALSTRRDLLPSDIADELAKLQDRVPPFDG
                     AIARAAIEAAFGHPVTDIFADFDPQPLAAASIAQVHAATLKNGREVVVKILRPGMREI
                     IDLDLEVLDYLAALADQYWTEARRLRPIELVSEYRKTVRDELDLLREAGNASQLKRNF
                     AGSPLLYVPEVFWDHCRVNVMVMERIHGVIVNDLAQLRARGTNIRKLAENGVEIFFTQ
                     VFRDNFFHADMHPGNIFVQIDDPENPRYAAVDFGIVGTLQARDQHYLAENFMAFFDRD
                     YARVAALHLESGWVPPGTRVDELESAVRTVCEPIFNKPLKEISFAQVLLRLFETARRF
                     DMQVQPQLILLQKTLFNIEGLGRQLYPELDLWQTAQPYLRKWMRARTSPRAMLRQVRA
                     QLPDVLLALQQVPQILQTTIRDAAEGRLRLRMENTAITELRDEMRRSNMGRDATAAAA
                     VLWLSGLIWLAMSTRSEWLGWVQMSAAIALFIYSRSRKPRAA"
     gene            5498..6376
                     /locus_tag="VGE92_10550"
     CDS             5498..6376
                     /locus_tag="VGE92_10550"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY1314309.1"
                     /translation="MSILRVSHRSEYRYQHPVELGPHRLMCRPRDSHDLRLLDTGITI
                     LPQPAELRWMHDVFGNSIAIATFSEPTAELVFESTFRAEHYPLPERTLVIDDYAVTLP
                     LSYSASEDVDLGSSKARQYPDPEHKLDAWVKGLLEKVPGHATMEVLMAMVTAIKADFV
                     YKRREEVGVQTPVETLDLASGSCRDFAVFMMDAVRFLGLAAQFVSGYLYDEALIDAGG
                     GLVGGGATHAWIQVYLPGVGWVEFDPTNALVGGRNLIRVAVAREAAQAAPLIGSFTGA
                     PGDFLALNVNVEVTAE"
     gene            6429..>7205
                     /locus_tag="VGE92_10555"
     CDS             6429..>7205
                     /locus_tag="VGE92_10555"
                     /inference="COORDINATES: protein motif:HMM:NF017930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314310.1"
                     /translation="MSPKPGKLADAQDPGAPRMFNSPHGIAPRTQLLSNGTYTVMLTV
                     AGSGYSRWRDLAVTRWREDATCDPWGSFVFLRDVADGQVWSAGYQPVGRDADSYSVAF
                     SEDRVGYIRRDGPILTSTEILVAGEDDAEVRRVSITNESNQSREIELTTYTEAVLAAP
                     DADSAHPAFSKLFVQTELLAEKGALLATRRGREPGEAQLWAAHVSALEGEAIGGLQFE
                     TDRARFLGRGRTLRDAVSVIDARPLSNSVGTVLDAVLALRR"
BASE COUNT         1329 a         2204 c         2329 g         1343 t
ORIGIN      
        1 gcgatggcca ggttacgctc ggacagagcg tgatgaagtc caatgcccgc aaagtgcggc
       61 ggctgtatgg cggcaaagtg ctggccggat tcgccggtgg gaccgccgat gctttcactt
      121 tgttcgagcg cttcgaagca aaactggaga aattcggcaa cctgacgcgc gccgccatcg
      181 aattggccaa ggactggcgc agcgatcgaa gcctgcgccg cttggaggcc ctgctgtgcg
      241 tggcggacac ggagagcacc tacgtgatct cggggaacgg cgatgtcata gaaccggagc
      301 atgacctgat cgccatcggc tcgggcggcg gtttcgcgca agcggcggcc acggcgttga
      361 tggcagggga tctttccgcc cgcgagatcg tcgaacgctc gttgaatatt gccgcggata
      421 tctgcattta caccaaccgc aatttgacga tcgaggagct gtagcggccc atgtcgcaga
      481 tgaccccgcg cgaaatcgtg gaagaactcg acaagcacat tgtcggccag aaggcggcca
      541 aacgtgccgt cgccatagcg ttgcgcaatc gctggcgacg cagccaggtg gctgaagcgc
      601 tgcgcgccga gatcacgccg aagaacattc tcatgatcgg acccaccggg gtcggcaaaa
      661 ccgagatcgc gcggcgcctg gcacgcctcg ccaaggctcc cttcatcaaa gtcgaggcaa
      721 cgaaattcac cgaagtaggc tatgttggac gcgaagttga ctcgatcgta cgcgatcttg
      781 tagagatctc cgtcaaaatg acccgggaag aggagatcgc caaggtgcgg caccgcgccg
      841 ccgatagcgc cgaggagcgg gttctcgacg tcctgttgcc gcgcccggcg gccggtttca
      901 ccggcgaggc gacgccgcgc gacagcgaga cccgccagcg ctttcgcaaa atgctgcgcg
      961 aggggcatct ggaggatcgc gagattgaaa tcgacgtgcg cgcggccccc atgggcgttg
     1021 aaatcatggc gcctcccggc atggaggaaa tgacccagca gctgcaaggc atgttccaga
     1081 atctgggcgc cggccgcacc cgccagcgca agctcaagat caaggaggcg ctcaaactct
     1141 tggtcgacga agaagcggcg aaaatgatca acgaagagga attgaagctt cgagccgtcg
     1201 agaatgcgga gcagaacggc atcgtgttca tcgatgagat cgacaagatc gcgcgccgcc
     1261 aggaaacggc cggcgccgat gtatcgcgcg aaggagtgca gcgcgatctg ttgcccctgg
     1321 tggaaggctg cacggtgaat accaagtacg gcacggtcaa gaccgatcat gtgctgttca
     1381 tcgcgtcggg cgcatttcac atgtccaagc cctccgatct gattccggaa ttgcagggac
     1441 gcttgcccat ccgcgtcgag ctcgaaccct tggtggtcga ggatttcgtg cgcattctga
     1501 ccgaaccgga tgcatctttg tgccgccaat acacggcgct gctcgcgacg gaagggttga
     1561 atttggagtt cgccgcctca ggcgtgcagc ggctcgccga agttgctttc gaggtcaatg
     1621 agcgcacgga gaatatcggt gcgcggcgcc tgcacaccgt gatggaacga ctgctcgaaa
     1681 ccgtgtcctt cgatgccgcg gaccgcaacg gcacgcacgt gttggtcaat cgcgaatacg
     1741 tcgagggcaa cctcggcgaa ctggtcaaag accaagactt gtcgcgctac attctatagc
     1801 gcggtgccat gcctcagccc accgaaatca aactgcgcac ccaatcgagg ctgctcgagg
     1861 tgcgcttcga tgatggatcg catttcgaac tgccgttcga atacctgcgg gtgttctcac
     1921 cgtccgcaga agtgaccgga cacggcgcag gcgaaggagt gctgcagacg ggcaagcaga
     1981 atgtgggcat cagcggcgtg gaacctgtcg gtaactacgc cctgcggctg ttgttcgatg
     2041 atggccacaa caccggcctt tacacctgga ccctgctgca cgagctcggc cgcgagcagc
     2101 gccgcaactg ggcccgctac ctcgagcgct gcgccgaaag cggacgctag cgccaccccc
     2161 gggtcggcta caatggccct ataaaccgag gggcgccatg gataactccg accgcaaggt
     2221 cgatttcgga tttgaaaaag tcgcctgggg cgacaaggcg cggcgcgtgc actccgtgtt
     2281 cgcgtcggtg gccggcaaat acgacgtcat gaacgatctg atgtccctcg gcgtgcatcg
     2341 gctgtggaag cgattcgcgc tttccttgac cgggctgcgc cccgggcagc gggccctcga
     2401 cgtggccggc ggcaccggcg atctggccgc cggaatgctc aagcaggtgg gcagatcggg
     2461 acatgtggtc ctgtccgacg taaaccccgc gatgctcgcg atcggccgcg accggctgct
     2521 cgatcagggg tgggtcgcga atatcgactg catcgtcgcc gacgccgaac gcctgccctt
     2581 cgatgacaac tccttcgact gcgtcaccat cggcttcggc ctgcgtaatg tgaccgacaa
     2641 ggcggcggcg ctcgattcga tgcggcgcgt cctgaaaccg ggtggccagc tgttggtgct
     2701 ggaattctcc acgccggtcg cgccgggctt gaagcccatc tacgatgcgt attccttcaa
     2761 ggtattgccg ctgctcggac gaatcgtcgc gaacgacgag tcgagctacc gctacttggc
     2821 cgaatcgatt cgcatgcatc ccgatcaaga gaccttgctc gagatgctgc gcgccgccgg
     2881 tttcgcccag gctcgctatc acaatttgac cggcggtatc gtcgctctgc atcgcggcta
     2941 caagatctag tatgcccgcg accgctgcct ggctcgcgtc ggccgaagcc ctgatcaacc
     3001 gcggtatcga tgcatcggcg caggccggcg cattggcccg gcggctgcaa ggcacggttt
     3061 tgcaaatcga tgttgacgga atcacgcacc tgcgcgccac cgcgtgcggc gcccggctgg
     3121 cgctgctcac agccgatgct gccgcggccg gcggcgccgg caccgcggac gcggcgattt
     3181 ccgggtcgct gcccgcgctt ctacggttgt tcaacggcgg tacgaaccca gcgtccggcc
     3241 gccgcgccgc gcccggcacg ccggcgcaaa ttcgcggcga tgcagaaatc gccaacctgt
     3301 atcgcgaact gtttctgctg gcgcggccgg atctggaaga agaattgtcg cgctggatcg
     3361 gtgactttgc ggcgcgccgc gtcgcgcagg ttgcggcgcg tgcgctcgcc ttcgcgcgcc
     3421 acgcgcggcg caccgcgggc gacaacatcg ccgagtactt gcaggaggaa agccgcgatc
     3481 tcgtcaacaa gacggagctc gaggaatttc ttcggggagt cgatacgttg cgcgaaaccg
     3541 ccgatagaat cgacgcccgg cttgcgtctc tggaacggcg tctggcaggc ggcgcctgat
     3601 gcggctgcga gttctggtcc ggctgctgca gatccaacgc gcgctggtga gacatggcct
     3661 cgatgatttc gtgcgtgcca cgcatttgta ccggccgttt cgctttctgg tgtacctatc
     3721 gccgtggact tggtttcaac gcagcagcgg cccgcgcggc gagcgcctgc ggttggcgct
     3781 cgaagaacta ggtccgatat tcgtcaagtt cggccaggca ttgtcgacgc ggcgcgattt
     3841 gcttccatcc gacatcgccg acgagctggc aaaacttcag gatcgcgtgc cgccgttcga
     3901 cggcgcgatt gcccgcgccg cgatcgaggc ggccttcgga cacccggtca cggatatttt
     3961 cgcggacttc gatccgcagc cgctcgccgc agcctcgatc gcccaggtgc acgcggcgac
     4021 gctcaagaac gggcgcgagg tggtggtcaa gatattgcgt cccgggatgc gcgagatcat
     4081 cgatctggac ctcgaggtgc tggattatct ggccgccttg gcggaccagt actggacaga
     4141 ggcgcgccgt ctgcgtccga tcgaactggt gagcgaatat cgcaagaccg tgcgcgacga
     4201 attagacctg ttgcgcgaag ccggcaacgc ctcgcaactg aagcgcaatt tcgcaggctc
     4261 gccgctgctc tacgtgcccg aggtgttctg ggatcactgc cgggtcaatg tcatggtcat
     4321 ggagcgcatc cacggtgtga tcgtcaacga tctggcgcag ttacgcgcgc gtggcacgaa
     4381 tatccgcaag ctcgccgaga atggcgtgga gatcttcttc acgcaggtgt tccgcgacaa
     4441 tttctttcac gccgacatgc acccgggcaa tatcttcgtg cagatcgacg atcccgagaa
     4501 cccgcgctac gcggccgtgg atttcggcat cgtcggcacg ctacaggctc gcgatcaaca
     4561 ttacctggcg gagaatttca tggcgttctt cgaccgggac tatgcgcggg tcgctgcctt
     4621 gcacctcgag tcgggttggg tgccgcccgg cacgcgggtc gatgagctcg aatccgcggt
     4681 gcgcaccgtg tgcgaaccga tcttcaacaa accgttgaag gagatctcgt tcgcgcaagt
     4741 actgctgcgc ttgttcgaaa cggcacgccg cttcgacatg caggtgcagc cgcaattgat
     4801 cctattgcag aagacgctgt tcaatatcga gggactcggg cggcaattgt atccggagct
     4861 cgatttgtgg cagacggcgc agccgtatct gcgcaagtgg atgcgtgcgc gaaccagtcc
     4921 gcgcgccatg ctgcgccagg tgcgcgcgca attgcccgat gtcctgctgg cgttgcaaca
     4981 ggttccgcag atactgcaga cgacgattcg cgacgcggcc gagggacgtc tgcgactgcg
     5041 gatggagaac acggcgatca cggagttgcg cgatgaaatg cgtcgcagca acatgggccg
     5101 cgacgcgacc gccgccgccg ccgtgttatg gttgtccgga ttgatctggc ttgcgatgtc
     5161 gacgcgcagc gagtggctgg ggtgggtgca aatgagcgcc gcaatcgcgc tgttcattta
     5221 ttcccgatcg cgcaagccgc gcgctgcatg actgcgatgc gcgcacctgt aggcatagta
     5281 ttacgcgggc atgcgacccg ttccgatcgc gctccgagcc gcattcgacg agtctagcca
     5341 tgctgcgtcg agtacgtcat tgcgcgaatc aggggccaaa gtacattctg ccgatccgcg
     5401 ccggcggtta tgatacgtcg agcggaactc gcaacctacc ccgtgagatc acacgcacga
     5461 tgtactcgat cgcagcaacg cccgaattga gaatttcttg agcatccttc gggtcagcca
     5521 tcgcagcgag taccgctacc agcaccccgt cgagctcggt ccgcaccggc tgatgtgccg
     5581 gccgcgagac agtcacgatc tgcggcttct cgacacggga attaccattc tgccgcagcc
     5641 ggccgagctt cgatggatgc acgatgtatt cggcaattcc attgcgatcg ccacgttttc
     5701 ggagccgacc gcggagctgg tgttcgaatc gacgtttcgc gccgaacatt atccgctccc
     5761 cgagcgcact ctcgtcatcg acgactacgc ggtcaccttg ccgctgagtt attcggcttc
     5821 cgaggatgtg gatctcggat cgtcgaaggc gcgtcaatat ccggatccgg agcataagct
     5881 ggatgcttgg gtgaagggtt tgctggagaa ggtccccggc catgcaacca tggaagtgtt
     5941 gatggccatg gtcacggcca tcaaagcgga tttcgtgtac aaacggcgcg aggaggtcgg
     6001 ggtccagact ccggttgaaa cgctggatct ggcgtccggc agctgccggg atttcgcggt
     6061 gttcatgatg gatgcggtgc gcttcttagg gcttgcggcg caattcgtct cgggatatct
     6121 ctacgacgaa gcgctgatcg atgccggcgg cggtctggtc ggcggcggcg ccacgcatgc
     6181 ctggattcaa gtgtatctgc ccggagtggg ctgggtggaa ttcgacccga ccaatgcgct
     6241 cgtcggcggc cgcaatttga tccgcgttgc cgtggcgcgc gaggcggcgc aggccgcgcc
     6301 gctcatcggc tcgtttaccg gcgccccggg cgatttcctc gctttgaacg tgaatgtcga
     6361 agtaacagcg gagtagttta aagccggccg gctattgcca acgtcttgaa agccggtatg
     6421 tttcatccat gtctccgaaa ccaggcaaat tagccgacgc tcaggatcca ggcgcgccgc
     6481 ggatgttcaa ctcgccgcat ggcatcgcgc cgcggacgca gttgctgtcg aacggcacct
     6541 ataccgtgat gctcacggtc gccggttccg gctacagccg ctggcgcgac ctcgccgtga
     6601 cgcgctggcg cgaggacgcg acgtgcgatc cctggggcag cttcgtgttc ttgcgcgacg
     6661 tcgccgatgg ccaggtgtgg tcggccggat atcagcccgt cggccgcgac gcggatagtt
     6721 acagcgtggc tttctccgag gatcgtgtcg gatacatccg gcgggacggg cccatcctca
     6781 cctccacgga gatcttggtc gcgggcgagg atgatgccga agtgcgccgc gtctccatca
     6841 ccaatgaaag caaccagtcg cgcgaaatcg aattgacgac ctatacggaa gcggtgctgg
     6901 cggcgcccga tgcggattcg gctcatccgg ccttttccaa gctgttcgtg caaaccgaac
     6961 tgctggccga gaaaggcgca ttgctcgcca cgcggcgcgg ccgggagccc ggcgaggcgc
     7021 agctgtgggc cgcgcatgtc agcgcgctgg aaggcgaagc catcggcggt ctgcaattcg
     7081 aaaccgaccg tgcccgcttt ctgggacgtg ggcgaaccct gcgcgatgcg gtgtcggtga
     7141 ttgatgcacg gcctctgtcc aactcggtcg gcaccgttct cgatgcggtg ctcgcattgc
     7201 ggcgg
//
LOCUS       DASWZT010000373         2061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_80368, whole genome shotgun sequence.
ACCESSION   DASWZT010000373 DASWZT010000000
VERSION     DASWZT010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80368"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VGE92_10560"
     CDS             <1..762
                     /locus_tag="VGE92_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314311.1"
                     /translation="VDLFACEISTLCRITTVLDSVRRLEEKFDVGSQAATAGGTAPAN
                     NQFSSQENYFPVFDSCARDLQTLEARVVVNITAFYTYMKAMRDGMRALGSLTAQFQGE
                     LLAGAGVAADPWHEGTRNVIYMLYLALESARKSVEDLVEFKPELIERTIVILISELEA
                     YRFLHTQFTDEADSRHQRIKLREADYRDLEPKLCSLVETNRAAERAKDSARPQGSAPP
                     VSQWLPAQLLLPELQKRYLAAIFTPVAENALTSRT"
     gene            complement(705..>2061)
                     /locus_tag="VGE92_10565"
     CDS             complement(705..>2061)
                     /locus_tag="VGE92_10565"
                     /inference="COORDINATES: protein motif:HMM:NF020683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactose oxidase-like domain-containing
                     protein"
                     /protein_id="HEY1314312.1"
                     /translation="LNAAKPAWAVAAPMKFPRRQHNATLLPDGTVLVTGGTRGGDFDN
                     LGLGQPVHTAELWNPSTGSWSELAAEAVDRCYHSTAVLLPDGRVFSGGGGEYAPVVGV
                     NQSNPPVNSHANAQIFSPPYLFRGARPLIGKTPVQVAYLADFDVETPLPAEISAVSWI
                     RLPSVTHSFDQNQRINFLKFTRGADKITVTAPPNGDVCPPGHYMLFLLNASKVPSIAA
                     IIQIASGTAAPIAAPARDVALPAAPVKFMSRAERLPTPLEQDAAIQAREMHPPVVIGV
                     TPTCPYGISACWGGAFEALSHLHGVRLVRPVPNAQDSTAYVYLAHEGLPDLNAWPMQF
                     ANIANGTHIFRGVEVILAGRVDARAGDSLQMEGNDLRPPLLLQPIEPTHKIQWDASAG
                     TTRPLDPEERNAFSRLQAAVRQAGGSLVATVTGPLIASNVGYVLEVRAFSATGVKIAA
                     R"
BASE COUNT          397 a          618 c          649 g          397 t
ORIGIN      
        1 gtggacttgt tcgcttgtga aatcagcacg ctgtgccgca tcaccaccgt tcttgattcg
       61 gtgcggcgcc tggaggagaa attcgacgtg gggtctcagg cggccacggc ggggggcacg
      121 gcaccggcca acaaccaatt cagctcgcaa gaaaactatt ttcctgtgtt cgacagctgt
      181 gcacgcgatc tacagacact cgaggcgcga gtcgtggtca atatcaccgc gttctacacc
      241 tatatgaagg cgatgcgcga cggaatgcgt gcgctcggct cactcactgc gcaattccag
      301 ggtgaattac tggcgggcgc tggcgtcgcc gcagatccct ggcatgaagg cacccgcaac
      361 gtcatttata tgctgtacct ggcgttggaa agcgcgcgca aatccgtcga ggatctggtg
      421 gagttcaaac cggaactcat cgagcgcacc atcgtcatcc tgatcagcga attggaagct
      481 tatcgatttc tccatacgca gttcacggac gaagccgaca gccgccacca gcgtatcaag
      541 ctgcgcgaag ccgactatcg agatctcgaa ccgaaactgt gctcacttgt cgagacgaac
      601 cgggcggcgg agcgggccaa ggattctgca cgaccgcaag gcagcgcgcc gccggtgtcg
      661 cagtggttgc ctgcccaact cttgttgccg gaattgcaaa aacgctacct cgcggcgatc
      721 ttcacaccgg tcgccgagaa cgctctgact tccaggacgt aacccacgtt ggatgcgatg
      781 agcgggccgg tgaccgtggc aacgagtgac ccgcccgcct gccgcaccgc cgcctgcagt
      841 cgtgaaaacg catttcgctc ctcgggatcc aatggccggg ttgtgcccgc actggcatcc
      901 cattgaatct tgtgagtggg ctcgatcggc tgaagcagca gcggcgggcg caaatcattg
      961 ccctccattt gaagcgaatc gcctgctcga gcgtccaccc gaccggcgag gatcacctcg
     1021 acgccgcgga aaatgtgcgt gccgttggcg atattggcaa actgcattgg ccacgcattt
     1081 aagtccggaa gcccctcgtg ggccaaatac acataggccg tcgagtcttg ggcgttcggt
     1141 accggtctca ccaggcgtac gccgtgcaag tggctgaggg cctcaaacgc cccgccccag
     1201 caggccgaga tcccgtaggg acaagttggt gtgacaccga tgacgaccgg tgggtgcatt
     1261 tccctcgcct ggatggcggc atcctgttcg agcggtgtgg gaagtcgttc ggctcgcgac
     1321 atgaatttaa ccggtgccgc gggcagcgcg acgtctctcg ccggcgcggc gatcggcgcg
     1381 gccgtgccgc tcgcaatctg aatgatcgcg gcgatggaag ggactttgct cgcgttcagc
     1441 aaaaacaaca tgtaatgccc cggcgggcag acatcgccat tgggcggtgc cgtgacggtg
     1501 attttgtcgg caccgcgtgt gaatttgagg aagttgatgc gctggttctg atcgaaagag
     1561 tgcgtcacag agggaagccg tatccagctc accgcgctga tctccgcagg tagcggcgtt
     1621 tcgacgtcaa agtccgcgag gtaggcgacc tggaccggcg ttttgccgat cagcggccgt
     1681 gcccctctaa atagataagg cggcgagaaa atttgtgcat tcgcgtggct gttgaccggg
     1741 ggattcgact gattcacacc gacgacgggt gcgtattccc cgccgccgcc actgaacacc
     1801 ctgccgtcgg gcagtagcac tgcggtcgag tggtagcatc gatcgaccgc ttcggccgca
     1861 agctcgctcc agctgccggt ggacggattc cagagttccg cggtgtgcac cggctgtcca
     1921 agtcctagat tgtcgaaatc gccgccgcgg gtgccgccgg tgaccagcac cgttccgtcg
     1981 ggcaagagtg tggcgttatg ctgtcggcgc gggaacttca tcggcgccgc gacggcccat
     2041 gcgggctttg cggcattgag a
//
LOCUS       DASWZT010000374         3987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_41821, whole genome shotgun sequence.
ACCESSION   DASWZT010000374 DASWZT010000000
VERSION     DASWZT010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41821"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VGE92_10570"
     CDS             complement(<1..485)
                     /locus_tag="VGE92_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314313.1"
                     /translation="MWLQQPDRVFRLAPLPALAAVYLQLKANMDAQDGEQVSEFLTQA
                     RRSIADLRPQNVILDMRFDGGGDYTKTAAFMADLPGMVPPEREAVGATLSDDRLPNSG
                     ISMSYATGLHDYLHGCHWFGSCFWVDWIYPIAVPTLKPDLAAPLTFALVAAGRDPAMQ
                     A"
     gene            complement(719..1624)
                     /locus_tag="VGE92_10575"
     CDS             complement(719..1624)
                     /locus_tag="VGE92_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY1314314.1"
                     /translation="MSSAAADKAGLVDPVSKYPKPPFPEQKQPWPGLVSKMQPKPDHG
                     EASYQGSGRLKGRRALITGGDSGMGRAAAIAFAREGADVAINYLPDEEPDAAEVVKLI
                     RDAGTKALAIPGDLRSEEFCRQLVAQAAAGLGGLDIVVSNAGRQQSHDSLLDISSEEF
                     DWTMTTNIYAPFWILKAAVPHLKPGSCIIATASEQAYDPSPDLYDYAQTKAATMNFVK
                     SLAKQLANKGIRVNGVAPGPVWTPLQISGGAKPEKQKQFGAHTPFGRAGMPGELAGIY
                     VLLADSQASFATGQIYGSSGGAGQP"
     gene            2010..3788
                     /locus_tag="VGE92_10580"
     CDS             2010..3788
                     /locus_tag="VGE92_10580"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY1314315.1"
                     /translation="MFGWLHLPKTPRRAGLGVVICSPFGYESICAHRSVREFAQAFAG
                     AGIPALRFDYLGTGDSGDVDENIDHIKLWSEDVVAASRELQVLADVDRICLLGIRLGA
                     LLATLAAGKSPSIDSMILVGPVVSGRRYASELRMTQLAGSALSGSLESTAEAAARRAG
                     PQPLEAGGFTLSAPTVEALTQVDLLSSAPPSVNSMLILDGDKLPSAKRWAERLSSSPI
                     RCDYRTMPGLIEMSMTAPQFAAVPHTMISACVEWLTAPAHAGAVSSPHPPQSSRDEES
                     PALEIMIGEPSDRRSRLTLIRERPVAIPAAGISLFGIVSEPRREEKRRRAVILLNPGA
                     DSHIGASRMYVSLARRWARRGYFALRVDLGGIGDSETRAGKRDDEVFPEEAIDDIRRT
                     IEYMRACYGATDITVAGLCSGAYHALRAAAAGLALNRILMVNPQNYFWKQGMTLEQIQ
                     LAEVVHNPGLYRRRMFSVEAWARVLKGEANIMRIGGIYLQRVRLAAEAVLRDAARRMR
                     VPLPNDLGWELERIVASGVGVTFLFARGEPGIDLLKLQAGSALARLEQQCHVRIVDSG
                     DHIFSRREPRALMGNLLSKELFASVR"
BASE COUNT          728 a         1227 c         1239 g          793 t
ORIGIN      
        1 atcgcttgca tcgcaggatc gcggcccgca gcgaccaagg caaatgtcag cggtgctgca
       61 agatcgggct tcaatgtcgg gaccgcaatc gggtaaatcc agtctaccca aaagcaggac
      121 ccgaaccagt ggcagccgtg aaggtagtcg tgcaggccgg tcgcgtagct catgctgatc
      181 cccgaattcg gcagacgatc gtcactgagc gtggcgccga ctgcttcacg ctccggcgga
      241 accatccctg gcagatccgc cataaatgcg gcggttttag tgtagtcgcc accgccgtcg
      301 aaacgcatgt cgagaatgac attctgtggc cgtaggtcgg cgatggagcg gcgcgcttgc
      361 gtgaggaact cgctgacttg ctcgccatct tgggcgtcca tattggcctt taactgtaga
      421 tacactgcag ccaacgccgg cagcggcgca agtcgaaaca cacgatcggg ctgctgcagc
      481 cacaagggca cggggaatac gcgcggtcgg gtgccacatc gcgacgcaga atcgccagat
      541 cctgcaattg cgcggcgcgc gaaccggccg cactggactc gcctagcgcc gaggccgagc
      601 ctacagcgag cgcgatgatc agcgccagcg ggatgcgatg tgccacttcg gtattggatg
      661 catcgatcgg taggaaggtt tgatatagcc ctgcaataca cgcagaagtg gcccgcttct
      721 agggttgtcc cgctccgccc gaagacccat atatttggcc ggtggcgaag cttgcttggc
      781 tatcggcgag caaaacgtat attcccgcca gttcccccgg catgccggca cggccaaacg
      841 gagtatgcgc gccaaattgc ttttgctttt caggttttgc gccaccgctg atctgaaggg
      901 gagtccatac tgggcctggg gccacgccgt tcaccctgat acccttgttc gcgagttgct
      961 tcgccagtga cttcacgaag ttcatcgtcg ccgccttcgt ttgcgcatag tcatacagat
     1021 ccggcgaggg atcgtaagcc tgctccgacg cagttgcgat gatgcaagag cccggcttca
     1081 aatgcggaac ggcggctttg aggatccaga acggcgcata gatattggtt gtcatcgtcc
     1141 agtcgaactc ttcgctcgaa atatcgagca acgagtcgtg cgattgctgg cgccccgcat
     1201 tgctcaccac gatgtcgagt ccaccaaggc ccgccgccgc ctgtgcgaca agttgccggc
     1261 agaattcctc gcttctgagg tcaccgggaa ttgccagtgc cttggttcca gcgtcgcgga
     1321 tgagcttcac gacttccgcg gcgtccggct cttcatcggg cagatagtta atcgcaacat
     1381 ccgccccctc tcgcgcgaag gcaatggccg ccgcgcgccc catgcccgaa tcgccaccgg
     1441 taatgagcgc ccggcgaccc ttgagtcgcc cgctgccctg gtagctcgcc tcgccgtgat
     1501 ctggcttggg ctgcatcttc gaaacgagcc ccggccacgg ctgcttttgc tcgggaaaag
     1561 gcggcttggg gtacttggag acggggtcca ccaggcctgc cttatccgcc gctgcgctgc
     1621 tcatactaca tcctcctgac attgtcgatt gcgttcaaga caatctatct tgcaatgcaa
     1681 agttagtacc gcccggctgc cgtgatcagc ggtgctggtc gcgaggggtc gtcagcgaag
     1741 tccgacccgc ctatggcgtg agacttaagc atccccgatc gtattttatg ttcagcaggc
     1801 gtgcgtgact ttccggcctt acagccgagt ttgacaatgt ctgaaaatct cggggatttt
     1861 cgctgacgcg tgtcgcgttg ttctcgaaag aaatccgaca cactccctta cacatgatcc
     1921 agtacgcaaa cgctgctgag agggatggtc ggatcacgac accggccggc gatgcccagc
     1981 ctctatactt ccggtcaggt gccaacaggt tgttcggctg gctgcacctg ccgaagacgc
     2041 cgcgccgtgc cggcctcggc gtggttatct gcagtccctt cggatatgag tcgatctgcg
     2101 cgcatcgcag cgtgcgcgaa tttgcccagg cctttgcagg cgccggcata cccgctctgc
     2161 gattcgacta cctcggcaca ggcgattcgg gtgatgtgga cgaaaatatc gaccacatta
     2221 aactgtggtc ggaggacgtc gtcgcggcga gccgcgaact gcaggtgctc gccgacgtcg
     2281 atcgaatctg tctgttggga attcggttgg gcgccctgct tgccacgctt gcggctggga
     2341 agagtccgag catcgactca atgattttag tcggacccgt ggtgagcggc cgacggtatg
     2401 caagcgagct gcgcatgacg caactcgcgg gcagcgcatt gagcggctcg ctggaatcaa
     2461 ctgcggaagc cgccgcgcgc cgcgcgggcc cgcagccgct cgaggcaggc ggcttcacct
     2521 tgtccgcacc caccgtcgaa gcgctgacgc aggtggactt gctgtcaagc gcgccgcctt
     2581 cggtcaattc catgctgatt ctcgacggcg acaagttgcc gtcggccaaa cgctgggccg
     2641 aacggctctc gagctcgccg attcgctgcg attaccggac catgccgggc ctcatcgaga
     2701 tgtcgatgac ggccccgcaa ttcgcggcgg tcccgcatac catgatctcg gcatgcgtgg
     2761 aatggttgac cgcgcctgct catgcaggcg cagtgagttc tccacacccg cctcaatcga
     2821 gtcgagatga ggaatcccct gccctcgaga tcatgatcgg agagccgtcg gaccggcgct
     2881 cgcggttgac cctgatccgt gagcggcccg tcgccatacc tgccgccggg atttccctgt
     2941 tcggcattgt cagcgaaccg cgtcgcgagg agaaacggcg ccgcgcggta atattgctga
     3001 atcctggcgc ggatagccac atcggcgcca gccgcatgta tgtatcgctg gcgcgacgct
     3061 gggcccggcg cggctatttc gcgctgcggg tcgacctcgg cggaatcggc gacagtgaga
     3121 ctcgcgccgg caagcgcgat gacgaagtgt ttccggaaga ggcgatcgat gacattcggc
     3181 gcacgatcga atacatgcgc gcgtgctatg gcgccaccga tattacggtg gccggacttt
     3241 gctccggcgc ctatcacgcg ttgcgcgcgg cggcggcggg ccttgcgctg aaccgcatct
     3301 tgatggtcaa tccgcagaac tatttttgga agcagggcat gaccctcgag cagatacagc
     3361 tcgcagaggt ggtgcataac ccaggcttgt atcgccgccg catgttctcc gtcgaggcgt
     3421 gggcccgggt tctgaagggc gaggcgaaca tcatgcgcat cggcggtata tatctgcagc
     3481 gcgtgcgact cgccgcggag gcggtattac gcgatgccgc gcggcgaatg cgcgtgccat
     3541 tgccgaacga tctcggctgg gagcttgaaa ggatcgttgc gagtggcgtg ggtgtgacct
     3601 tcctgtttgc acgcggcgag cctggtatcg atttgctcaa gctgcaggcc ggctcggcac
     3661 tggcgcgcct cgagcagcag tgccacgtgc gcatcgtcga cagcggcgac cacatcttca
     3721 gcagacgcga gccgcgggcg ctgatgggaa atctgttgag caaggaactc ttcgccagcg
     3781 ttcgttgacc ttctaggccc ctggacttga gctaaatcag accatttgcg gaaaatcact
     3841 tcaatcgcgc agagtgccgg ggtggacgca ggcaaatagg cgctggatat cccgcatggc
     3901 ctgaacgcgg gctccccgcc cggtttccgc gctcaggccg cactgcgcga gcgcttcggc
     3961 gcagcctttc tcggtgcttt tgtggct
//
LOCUS       DASWZT010000375         6016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_12748, whole genome shotgun sequence.
ACCESSION   DASWZT010000375 DASWZT010000000
VERSION     DASWZT010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6016
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12748"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VGE92_10585"
     CDS             complement(<1..951)
                     /locus_tag="VGE92_10585"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY1314316.1"
                     /translation="MLEDLRALVEFAHAGSIAGAANRLFRTPSAITRQVQRLEAALGA
                     ELLDRSVKPPRLNSLGSRVLEQARDLLQRTEALKSITSSDAEPHGLLRIGLAHALAEG
                     TLIEPIQALTEKYPKVRLRLSSELTGELIHRLLAGELDVAAVLLPEGRTAPAPLLTNI
                     IARDRMEIVQGVAGNVEGDWKSLCRAPWVLNPPGCPLRASLIDRMERAGFTPVIAAEI
                     HNMHMQLAFVQSGYGVGLLPARFIARNQSIDPVKVLHPAAFELHRSVAIVRVAQLGAL
                     GKAVELLEHGFRQLFEAADTPKPVTRHRRGVPARIGRLIRD"
     gene            1067..2038
                     /locus_tag="VGE92_10590"
     CDS             1067..2038
                     /locus_tag="VGE92_10590"
                     /inference="COORDINATES: protein motif:HMM:NF008024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEY1314317.1"
                     /translation="MPHAIQIRQTGAPEVLRWTAVDVGVPGSGQVRLRQAAAGLNYID
                     VYHRTGYYPQPLPFIPGLEGAGTVEAIAPDVRSLKVGDRVAYAGCIGGYSQVRLIEAD
                     RLVRLPEAISFDQAAAMMLKGMTAQVLLQQVYSVKAGDLILVHAAAGGTGLMLCQWAA
                     ALGATVIGTVSTQAKAELAHAHGCSHTILYTKQDFVAEVSRISGGEKLPVVFDSVGKD
                     TFLRSLDCLRPRGLMVTFGQASGPIDPIAPVLLSQKGSLFLTRPTLFHYIERRDALEA
                     SANELFEVVASGKVRINVNQRFALKDAANAHKALAARTTSGSTILTI"
     gene            2062..2442
                     /locus_tag="VGE92_10595"
     CDS             2062..2442
                     /locus_tag="VGE92_10595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HEY1314318.1"
                     /translation="MSKTAITSPELAPPVGPFSQAIEAGGFLYFSGQVGQDPATGKVV
                     EGGIVAETERVLRNLSALLKTAGKSFDHVARAGVYLTSMSDFGAMNGIYAKYFRQPFP
                     ARTTIAVAALPLGACVEIDLVVKA"
     gene            2466..3380
                     /locus_tag="VGE92_10600"
     CDS             2466..3380
                     /locus_tag="VGE92_10600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEY1314319.1"
                     /translation="MKYAFHIVDVFSSTPFGGNQLAVLPEAAGISAEGMQKIAREFNF
                     GETTFVLPKNDPANTCRVRIFTPRAELDFAGHPSVGTACALVMKQHVGPGDPIRLILE
                     ENVGPVTVDVAQRSGGFHGTLTLSGKIEAPTGAPSATDLAAVLSIEPAEVSQVFFAGV
                     GVPFCFAQLNSNEVVDRAALNRAAWAATLARAWSPHIFFFAGNLRDGAKLHARMWAPA
                     LGVEEDPATGSACAALVGAMAAKPDFGGMTYRLSIQQGVSMGRRSDIEAQARKRDGVV
                     TSVSVGGATTYIASGEIDVPPFALETRG"
     gene            3403..4161
                     /locus_tag="VGE92_10605"
     CDS             3403..4161
                     /locus_tag="VGE92_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314320.1"
                     /translation="MKIMAILIAVVALICGAKALDNTSNSAAAMAVADASRPDSDTTR
                     DADRKPAQTLMFVGIKSGDTVADYVADSGYFTRLFASVVGSKGHVYAVEPTAFFKYPH
                     FVKEAAELQGYAVTHPNITLTTAAALEGLKFPEKLDLFWISQNYHDLHDEFMGPVDTA
                     AFNKAVYSALKPGGVYVILDHSAASGAPANVTETLHRIESSTVRREVEAAGFKFDSES
                     SILANPADPRTAKVFDPTIRGHTDQFILKFRKPK"
     gene            4281..5894
                     /locus_tag="VGE92_10610"
     CDS             4281..5894
                     /locus_tag="VGE92_10610"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314321.1"
                     /translation="MSKWRSVGIIFLASVCGVAYTGERPRAQEPPHAVEQPSPAAALN
                     SQLCKIDIKGEKKDQWMRLPKPESSVTEHRFGVGGKSFDYTATAGTLIIRDDEDKPIA
                     SIGYVAYVRRDLKGGGARPLMFAFNGGPGSSSLWLHMGVLGPKRVVVSDPGPTPAGPY
                     RTVDNEFGVLDKSDLVMIDPVGTGLSHAVCDHKDNEFWAVDPDIDSVSRFIAQYVSDN
                     NRWTSPKYLLGESYGTTRGAAIVNYLRGRRSFAFNGLILVSVATDLEAIFAELPGNDR
                     PYSVYLPGYAAVAWYHHMLPNQPAALEPFLTEVRAFAAGPFTAALLKGDALTDAEREA
                     VVGKMHEYTGLSTEYLKAANLRVSEIAFAHELLKSQRKTLGRLDGRFVGPTADPLEKY
                     TDYDPQSAAISAAFAAAFLDYYHGDLKFGQGSTYQTTNFGIGDQWKWTHKTANGEQPM
                     VNSGVDLAEALVKDANLRVLVLNGYYDLATPFSATEYVMTHLGLPPGLGARIEMKYYE
                     AGHMMYVHPPSMAKMKRDLDAFIDSTARP"
BASE COUNT         1158 a         1858 c         1876 g         1124 t
ORIGIN      
        1 atctcggatc aaccggccaa tccgtgcggg cacacctctt cggtgcctcg tcacgggctt
       61 cggggtgtcg gctgcctcaa acaattgccg aaacccgtgt tccaacagtt cgacggcctt
      121 ccccaaggcg ccgagttgcg caacccggac tatcgcaacg gagcggtgca gctcaaacgc
      181 tgcgggatgc aatactttca ccggatcgat agactgattc ctggcaataa agcgagcggg
      241 tagcagcccg acaccgtaac cggactgaac gaacgccagc tgcatgtgca tgttgtggat
      301 ttcagcggca atcacggggg tgaagccggc acgctccatc cgatcgatga ggctggcccg
      361 cagcgggcac ccgggcggat tgagcaccca gggtgctcgg cacaaacttt tccaatcacc
      421 ctcgacgttg cctgcgacgc cttgaacgat ctccatgcgg tcgcgagcga tgatattcgt
      481 cagaagaggc gcaggcgcgg ttctaccttc gggcaacagc acggcggcaa catcgagctc
      541 gcccgcgagc agccggtgaa ttaactcacc ggtaagttcg ctcgacaacc gcagtcggac
      601 cttggggtat ttttcagtca gcgcctggat aggttcgata agcgtcccct cggcgagggc
      661 atgcgcgagc ccgatgcgca acaggccgtg aggttcggcg tcgctcgagg tgatagattt
      721 cagcgcctcg gtccgctgca gcagatcgcg cgcttgttcg agcaccctgg agccgagcga
      781 gttcagacgc ggcggcttca ccgagcggtc gagcaattcg gcacccagtg cagcctccag
      841 cctttgcact tgccgggtga tcgccgaggg cgtccggaat agacgattgg ctgcaccggc
      901 gatggacccg gcatgggcga actctacgag ggcgcgtaaa tcttcgagca cgggggtctc
      961 cttggatcgt gcctgaatag tactattatc gtgataataa tgaagtagtg gaattttctg
     1021 tcttgcatca agatatcagc aagttcatgt ttttgcggaa atcggcatgc cgcatgcgat
     1081 ccaaattcgg cagacgggcg ctccggaggt tctccgctgg acagcggtcg atgtcggtgt
     1141 gcccgggtcg ggtcaggtgc gactgcgcca ggcggcggcg ggcctcaact acatcgacgt
     1201 ttatcatcgc accggatact acccgcagcc gctgcccttt attccgggcc tcgaaggcgc
     1261 agggaccgtc gaggcaatcg ccccggatgt gcgaagtctg aaagtcggcg atcgggtggc
     1321 ttatgccggt tgtatcggcg gctattcgca ggtgcggctc atcgaggccg atcggctggt
     1381 gcgattgccg gaggccatct ccttcgatca agccgccgcg atgatgctca agggcatgac
     1441 tgctcaggtg ctgctccagc aggtgtattc cgtcaaggcg ggtgacttga tcctggtgca
     1501 tgccgcggcc ggcggaacgg gtctcatgct ctgccagtgg gcagccgccc tcggcgcaac
     1561 ggtcatcggt accgtgtcga cgcaagctaa ggccgaacta gcccatgccc acggctgcag
     1621 ccacacgatc ctctatacga aacaggattt cgtagcggaa gtttcccgca tctcgggcgg
     1681 ggagaaactg ccggtggtct tcgattccgt cggcaaggat acattcctgc gctcgctcga
     1741 ttgtctaaga ccacgcggat tgatggtgac gttcgggcaa gcctccggtc ctatcgatcc
     1801 cattgcgccc gttctgctgt cgcagaaggg ttcgctgttt ctgacgcgcc caaccctctt
     1861 ccactacatc gagcggcgcg acgcgctcga agcttcagcg aacgagctgt tcgaggtggt
     1921 ggcctcggga aaggtgcgca ttaacgtcaa tcagcgcttt gcgctcaagg atgctgccaa
     1981 cgcccacaag gcattggcag cgcgcacaac ctcgggctcc accatcctga ccatctaacc
     2041 cggcagccac aggagttttt catgagcaag accgcaatta cctcgcccga actcgcgccg
     2101 ccggtcggcc cgttttccca ggcgatcgaa gccggtggtt tcctctactt cagcggccag
     2161 gtcggccaag acccggccac cggcaaggtg gtcgaggggg gtattgtggc cgagacagag
     2221 cgcgtcttgc gcaatctctc ggcgcttctc aagacggccg gcaagagttt cgatcacgtt
     2281 gcgcgcgccg gcgtgtatct caccagcatg agtgacttcg gcgcgatgaa cggcatctac
     2341 gccaagtatt tcaggcagcc ctttcccgcc cgcacgacga tcgcggtggc cgcgctgccg
     2401 ctgggggcct gcgttgagat cgacctcgtc gtcaaggctt gagacgacgc acggaggtcg
     2461 atctgatgaa gtacgcattt cacatcgtcg atgttttcag ttcgacaccg ttcggcggca
     2521 atcaactcgc ggttctgccg gaggcggccg gtatttcggc cgagggcatg cagaagatcg
     2581 cccgtgaatt taatttcggc gaaacgactt tcgtgctgcc taagaacgat cctgcgaaca
     2641 cgtgccgggt gcgcatcttc acccctcggg cggagctcga ctttgcgggg cacccctcgg
     2701 tcggcacagc ctgtgcgctg gtgatgaaac agcacgtagg gcccggtgat ccaatccgcc
     2761 tcattctgga ggagaatgtc ggtcccgtga cggttgacgt cgctcagcga agcgggggat
     2821 tccacggcac gttgacgtta tcgggaaaga tcgaggcacc gaccggcgcg ccgtctgcga
     2881 ctgatctcgc cgccgtcctc tccatcgagc ccgccgaggt gagccaagtc tttttcgcgg
     2941 gcgtcggtgt tccattctgt tttgcgcaac tgaactcgaa cgaggtcgtc gatcgggcgg
     3001 cgctcaatcg ggctgcctgg gcggcaacgc tcgcgcgcgc gtggtctccc cacatcttct
     3061 tcttcgcggg aaatctacgg gatggcgcca agctccatgc gcgcatgtgg gcacccgcgc
     3121 tcggcgtcga agaagacccc gcgacgggca gcgcgtgtgc cgccctcgtc ggggcgatgg
     3181 cagcgaagcc cgattttggc gggatgacct atcgcctttc gatccagcaa ggcgtctcga
     3241 tggggcgccg cagcgatatc gaggcgcagg cgcgcaagcg cgacggcgtg gtgacctctg
     3301 tcagcgttgg cggcgcaaca acctacatcg caagcggcga gatcgatgtg ccgccgttcg
     3361 cgcttgagac gcggggctaa accagaatat tcaggagtcg atatgaaaat catggctatc
     3421 ttgatcgcag tcgtcgcgct gatttgcggg gcgaaagcct tggacaacac aagcaattca
     3481 gccgccgcca tggcggtcgc cgatgcgtcg cgcccggact ccgacaccac acgggatgct
     3541 gaccggaagc cggcgcaaac tttaatgttc gtaggcatta aatcggggga cacggttgcc
     3601 gactatgtcg ccgactcagg ctatttcacg cgactcttcg ccagcgtcgt cggctccaag
     3661 ggtcacgtct acgcggtgga gcccacggca ttcttcaagt acccgcattt cgtcaaggaa
     3721 gccgcggagc tgcagggcta tgccgtcacc catcccaaca taacgttgac gactgccgcg
     3781 gcgctcgaag gattaaagtt ccccgagaag ctcgacctgt tctggatttc gcaaaattac
     3841 cacgacctcc acgacgagtt catgggcccg gtcgacaccg ccgcattcaa caaggccgtc
     3901 tacagcgcgc tgaagcccgg tggcgtctat gtaattctcg atcattccgc cgcttcgggt
     3961 gctcccgcca atgtaaccga gaccttgcat cgcatcgagt cctcgaccgt gcgccgcgag
     4021 gtcgaagctg cggggttcaa gttcgactcc gagagttcga ttctcgccaa tccggcggac
     4081 ccccgcacgg ccaaagtgtt cgacccgacg attcgcggac acaccgacca gttcatcctg
     4141 aagttccgca aacccaagta atgaggatgg cggccttccg gggagcctct caggagcgcg
     4201 gcggacgaac aagccggcca gagacggcgg aaaaataata gaatgccgat gcggtatggg
     4261 aaccctgggg gaggcgcgca atgtcgaagt ggcggtcggt gggcattatt ttcctggcga
     4321 gtgtgtgcgg tgtggcgtac acgggcgaac gcccgcgcgc acaggagcca ccccatgcag
     4381 tcgagcagcc gagccccgcg gcggctctca actcgcagct gtgcaagatc gacatcaagg
     4441 gcgagaagaa agatcagtgg atgcggctgc cgaagccgga gagctcggtc accgagcatc
     4501 gcttcggcgt cggcggcaag tcatttgatt acaccgccac cgcgggcacg ctgatcatcc
     4561 gtgacgacga agacaagccg atcgcgagca ttggctacgt cgcttatgtc cggcgcgatc
     4621 tcaagggcgg cggcgcgcgg ccgctgatgt tcgcattcaa cggcggaccg ggatcctcat
     4681 cgctgtggct gcacatgggc gtgctcggac cgaagcgtgt cgtggtgtcg gatcccggcc
     4741 cgacgccggc gggcccgtat cgcacggtgg acaacgaatt cggcgtgctc gacaagagcg
     4801 acctggtgat gatcgatccg gtcgggacgg gcctgagcca cgcggtctgc gatcacaagg
     4861 acaacgaatt ctgggcggtc gatccggaca tcgattccgt cagccgcttc atcgcgcagt
     4921 acgtgagcga caacaatcgc tggacctcac cgaagtacct gttgggcgag agctacggca
     4981 ccacgcgtgg ggcggccatc gtcaactacc tgcggggacg acgttcgttc gccttcaacg
     5041 gcctgatact cgtgtccgtc gccaccgacc tcgaggcgat attcgccgag cttcccggga
     5101 acgaccggcc ttactcggtg tacctgccgg ggtatgccgc ggtggcctgg taccaccaca
     5161 tgctgcccaa ccagccggcg gcgctcgagc cgttcctcac cgaggtgcgc gccttcgccg
     5221 ccggcccgtt cactgcggcg ctgctgaagg gcgatgcgct caccgacgcc gagcgcgagg
     5281 cggtggtcgg gaagatgcac gagtacaccg ggctgtcgac cgagtacctg aaggccgcca
     5341 acctgcgggt gtcggagatt gccttcgcgc acgagctcct gaagtcgcag cgcaagaccc
     5401 ttggccgcct cgacggtcgc tttgtcggac cgaccgcgga tccgctcgag aaatacaccg
     5461 actatgaccc gcagtcggcg gcgatcagcg ccgcctttgc ggcggcgttc ctcgactact
     5521 atcacgggga cttgaagttc gggcagggca gcacctacca gaccaccaac ttcggcatcg
     5581 gggaccagtg gaagtggacc cacaagaccg cgaatggcga acaaccgatg gtcaattcag
     5641 gcgtcgacct cgccgaggcg ctggtcaagg atgcgaacct gcgcgtgctg gtgctgaacg
     5701 gctactacga tctcgccacg ccgttctccg cgaccgaata cgtcatgacg catctgggtc
     5761 tgccgccggg gctgggcgcg cgcatcgaga tgaagtatta cgaggccggc cacatgatgt
     5821 acgtgcaccc gccgtcgatg gcgaaaatga agcgcgacct cgacgcgttc atcgactcaa
     5881 ccgcgcggcc gtaagccaag cgagccgctc acataaggtg cttgagcggc aagccgacga
     5941 atcacaaaat cgtgctcaaa cggcactatc atcgtgataa taatgaattt gtgtaatttt
     6001 ctatcttgca taaaaa
//
LOCUS       DASWZT010000376         2439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_32174, whole genome shotgun sequence.
ACCESSION   DASWZT010000376 DASWZT010000000
VERSION     DASWZT010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32174"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1386)
                     /locus_tag="VGE92_10615"
     CDS             complement(<1..1386)
                     /locus_tag="VGE92_10615"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEY1314322.1"
                     /translation="MDKAFSPVDIEARIYAKWEACGYFAPSGRGPAYSIVIPPPNVTG
                     TLHMGHAFQDTIMDALIRHRRMQGFDTLWQPGTDHAGIATQMVVERQLNAQGSSRVAI
                     GRDAFVERVWQWKEQSGGQIANQMRRLGASVDWQRDRFTMDAGLSQAVTEVFVRLYQE
                     GLIYRGKRLVNWDPVLKTALSDLEVVAEEESGSLWHLRYPLADGTGCLVVATTRPETM
                     LGDAAVAVHPDDERYRHLIGRRVRLPLTPRDIPVIGDAYVDPQFGSGCVKITPAHDFN
                     DYEVGQRHGLPLINIMNPDASLNDAVPAAYRGLERAVARTRILADLDALGLIERVEPH
                     KLTVPRGDRSNAVLEPLLTDQWFVDIKPLAEPAIRAVETGDIRFVPEQWSAVYFEWMH
                     KIKDWCISRQLWWGHRIPAWYDPDGRWFVARTEAEARAVHHIDPATPLKQDEDVLDTW
                     FSSALWPFSTQG"
     gene            complement(1379..1801)
                     /locus_tag="VGE92_10620"
     CDS             complement(1379..1801)
                     /locus_tag="VGE92_10620"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEY1314323.1"
                     /translation="MTERVDFYVLKSNNAKQRWVFACRLIEKAYLRDLRVVIVNDTPA
                     DAKALDDLLWTFNERSFVPHHIADTHITVEPAVPAGPELLVNLTAGLPAQWERYGRVA
                     EIIDADEERRRLGRERFKAYRDFKVSVETHQLDESADG"
     gene            complement(1798..>2439)
                     /locus_tag="VGE92_10625"
     CDS             complement(1798..>2439)
                     /locus_tag="VGE92_10625"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314324.1"
                     /translation="DEMKFDMSGAAAVLGAMRAIAELKLPINLVVIVATCENMPSGTA
                     VKPSDIVTTMSGQTVEILNTDAEGRLVLCDAITYSRRFKPAAVVDVATLTGACIVALG
                     NHFSGLMSNDEALADELQSAGIRADDRAWRMPIGEEYVEQLKSNFADIANIGGREGGA
                     CTAASFLSKFAKDLHWAHLDVAGTAWLGGANKGSTGRPVPLLVDFLIHRVRAT"
BASE COUNT          445 a          765 c          767 g          462 t
ORIGIN      
        1 tccttgggtc gagaacggcc acagcgcgga ggagaaccag gtgtcgagca catcttcatc
       61 ctgcttgagg ggcgtcgctg ggtcgatgtg atggacggcg cgggcttccg cctccgtgcg
      121 ggcgacaaac cagcggccgt cgggatcgta ccaggcgggt atgcgatgtc cccaccaaag
      181 ctgtcgactg atgcaccagt ctttgatctt gtgcatccac tcgaagtaaa cggccgacca
      241 ctgctcgggg acgaagcgaa tgtcgccggt ttcaaccgcg cggatggccg gctccgccaa
      301 cggtttgatg tcgacgaacc attgatcggt gagcagcggt tcgagcacgg cgttgctgcg
      361 atcgccgcgc ggcacggtga gtttgtgcgg ttcgacgcgt tcgatcaacc cgagggcgtc
      421 caagtcggcg agaattcgcg tgcgcgcgac agcgcgctcg aggccgcgat aggcagcggg
      481 aaccgcatcg ttgagcgatg cgtccgggtt catgatgttg atgagaggca ggccatgacg
      541 ttggcccacc tcgtagtcat tgaaatcatg cgccggcgta atcttcacgc agccgctgcc
      601 gaattgcgga tccacatagg cgtcgccgat gaccggaata tcccgcgggg tcaggggcag
      661 gcggactcgg cgaccgatca aatgccgata gcgctcatca tccgggtgca ccgcgactgc
      721 ggcatcgccc aacatcgttt ccggtcgggt ggtcgcgacc acgaggcagc cggtgccatc
      781 cgcgagggga tagcgcaggt gccagaggct gccggattct tcctccgcga ccacctccaa
      841 gtcggagagc gcggttttca gcacgggatc ccagttcacc aagcgcttgc cgcgatagat
      901 caggccttcc tgatagagac ggacgaagac ttcggtcact gcctgcgaca gacccgcgtc
      961 catggtgaat cggtcccgct gccaatcgac ggaggcgccg agccgccgca tctgattggc
     1021 gatttggccg ccggattgct ccttccactg ccacacccgc tcgacgaacg cgtcgcgtcc
     1081 gatcgcgacg cggctcgagc cttgcgcatt caattgacgt tcgaccacca tctgcgtggc
     1141 gatgcccgca tggtcggtac cgggctgcca gagcgtatcg aagccttgca tgcgccggtg
     1201 ccggatgagc gcgtccatga tcgtgtcctg aaacgcatgt cccatgtgca gggttccagt
     1261 cacattcggc ggcgggatga cgatcgaata ggcaggtccc cggccgctcg gcgcgaaata
     1321 tccgcaagcc tcccatttgg cgtaaattcg cgcttcgatg tcgacgggag agaatgcctt
     1381 atccatccgc gctctcgtcc aactgatgcg tctcgacgct gaccttgaaa tcgcggtacg
     1441 ccttgaagcg ttcgcggccg aggcggcgcc gctcctcatc ggcgtcgatg atctcggcga
     1501 cgcggccgta gcgttcccac tgcgcgggca gacctgcggt caaattcacc aacaattccg
     1561 gcccggcggg caccgccggt tccaccgtga tgtgcgtgtc tgcgatgtgg tgcggaacga
     1621 aggagcgttc gttgaacgtc cacagcagat cgtcgagagc ttttgcgtcc gccggcgtgt
     1681 cattgacgat gacgacgcgc aaatcccgaa ggtaagcctt ctcgatcagc cggcaggcaa
     1741 atacccagcg ctgtttggcg ttgttgcttt tcagtacgta gaaatccacc cgctcggtca
     1801 tgtcgcccga acgcgatgga tcaggaaatc gacgagcagg ggcacgggcc gtccggtact
     1861 gcctttgttg gcgccgccca gccacgcggt cccggcgaca tccaaatgcg cccagtgcaa
     1921 atccttggcg aatttagaca agaatgaggc ggcggtgcag gcgccgccct cgcggccgcc
     1981 gatgttggcg atgtccgcga agttgctttt cagctgctcg acgtactcct cgccgatcgg
     2041 cattcgccaa gcacgatcgt cggcacggat gccggccgac tgcaactcat ccgcgagcgc
     2101 ctcgtcattg ctcatcaagc cgctgaagtg attgccgagg gcgacgatgc aggcgccggt
     2161 gagcgtcgcc acgtcgacca cggcagccgg cttgaagcga cggctgtagg tgatggcatc
     2221 gcacagaacc agccgtccct cggcatcggt gttgaggatc tccaccgttt gccccgacat
     2281 ggtggtgacg atatcggaag gtttgacggc ggtgccgctc ggcatatttt cgcacgtcgc
     2341 aactatcacc acgaggttga tcggcaattt caactcggcg atcgcgcgca tcgcgcccaa
     2401 caccgcggcc gcgccgctca tatcgaattt catttcatc
//
LOCUS       DASWZT010000377         4143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_95036, whole genome shotgun sequence.
ACCESSION   DASWZT010000377 DASWZT010000000
VERSION     DASWZT010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95036"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VGE92_10630"
     CDS             <1..831
                     /locus_tag="VGE92_10630"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEY1314325.1"
                     /translation="AAVAPLPPSAAQIARVRSGAIALTAALDFRDADVVFLALHGDAG
                     EDGRIQALLDLAGLAYTGSNHIASAAAMDKDLSKRLFRSVGVPTPDWLMAPAASDEVG
                     RSLGWPAIVKPNKQGSTVGLSVVREAAELQDAIDRALRFDDEVMIERFIPGREFTVGI
                     LDGLALPVGEIIPPGEVFDYESKYQAGAAREVFPAVIAAEESALLQEFARRVHRVLKL
                     GAYSRTDFRRDPAGEFWCLEANSLPGMTATSLLPQAAKAAGIVFAELLDRICRGALKA
                     "
     gene            complement(836..1543)
                     /locus_tag="VGE92_10635"
     CDS             complement(836..1543)
                     /locus_tag="VGE92_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314326.1"
                     /translation="MAGTRAPLSVLGAWFAFCLGCWSPRLAMSSPLVSRAELCVTEGA
                     VEELPGTRLSVGTAKMRAYLNRSTQQSIAARVTYVGPTATDVPLASGELRRQFGFKLR
                     ALDACNLVYVMWRIEPAPRLVVSVKTNPGQSASSECGNRGYRNLKPQHAAAIVDLRPG
                     ATHTLRAQMNADDLRVFIDEAIVWQGSLGRDAAGLQGPVGIRSDNMRLVMELRAGEGR
                     GTASQAATCHSEADAAE"
     gene            complement(1550..2275)
                     /locus_tag="VGE92_10640"
     CDS             complement(1550..2275)
                     /locus_tag="VGE92_10640"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY1314327.1"
                     /translation="MQLFRSLLFTTYMMASACLFGAAMALGFWLPYRVQFAIARTWAR
                     ILFWVLERLCGLKYVVEGRERIPSGNHIVMSNHTSAWETVAQFLIFPPQVWVLKRELL
                     WIPFIGWGLKLLRPIAINRGEGHRAVNQVIEQGKSRLADGLWIIIFPEGTRVVAGEKR
                     KFGVSGALLAIASGKSVVPLAHNAGTFWPRRGFVKKPGTIRVVIGEPIEPAGKDPRQL
                     NEEVRQSIEAGLARIATLANGPG"
     gene            complement(2284..>4143)
                     /gene="glyS"
                     /locus_tag="VGE92_10645"
     CDS             complement(2284..>4143)
                     /gene="glyS"
                     /locus_tag="VGE92_10645"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEY1314328.1"
                     /translation="VERRGPPLKLCFDAHGAPTQAAIAFAKNCNVPLAELEQLQTDKG
                     AWLVFRGTERGTATANLLGHIVNQAVATLPIAKRMRWGSRSTEFVRPVHAVVLLYGDA
                     VVSAEVLGLPTGRTTFGHRFHAPKPIALKSAKSYEGRLRRAKVVADFAARRELIRDGV
                     IEAAARCNGGTALIEAALLDEVTALVEWPVPIAGQFEQRFLSLPREVVIATVQDHQRY
                     FAVQGAEGRLSGWFITVANIESRDPAKVRQGNERVVRPRLSDAAFFWDQDRKLSLDEH
                     AAKLGGVTFQTRLGSYADKTLRVKALAQLIGGRIGAGDYVARAALLAKADLMTAMVNE
                     FPELQGTMGRYYALAQGEPQELALALEEHYHPRFAGDSLPTTKTGQCIALADKIDTLA
                     GIFAIEQRPTGAKDPFGLRRAALGLLRIMLEERLDLDLFELLTTAAAAQPVARPGVVD
                     EVYDFLIERLKGMLLEEPGTSAEMLDAVFANRPGSPLDAATRLQALKEFLLLPEAGIL
                     AAINKRIANILKKAALGPGVSVDAAALTESAERQLHQALTELRTPLQEATRQRRYAQG
                     LRSLVGLRAPVDEFFERIMVMDENIERRNNRLALLRDVQLLLGGVADLSRLPG"
BASE COUNT          737 a         1384 c         1299 g          723 t
ORIGIN      
        1 gccgccgtgg caccgctgcc cccatccgct gcgcagattg cgcgcgtgcg cagcggagcg
       61 atcgccttga ccgcggccct cgatttccgc gacgcggatg tggtgttcct agccttgcac
      121 ggcgacgccg gcgaggacgg ccgcattcaa gcgctgctcg atctggcggg gttggcgtat
      181 accggtagca atcacatcgc gagcgctgcg gcgatggaca aggatttgag caagcgcttg
      241 ttccgctccg tcggtgtgcc gacgcccgat tggttgatgg cgccggctgc gagcgatgag
      301 gtgggcaggt ctctcggctg gccggcgatc gtcaaaccca acaaacaggg ttcgacggtg
      361 ggcttgagcg tggttcgcga agcggcggag ttgcaagacg cgatcgatcg ggcgctgcgc
      421 ttcgacgatg aggtcatgat cgagcggttc attccgggcc gcgaattcac cgtcgggata
      481 ctcgacggtc tcgctttgcc ggtcggcgaa atcatccccc cgggcgaggt gttcgactac
      541 gaatccaagt atcaggccgg cgctgctcga gaagtcttcc cggccgtcat agccgcggag
      601 gagtccgccc tgctccagga atttgcgcgt cgtgttcacc gagtgctgaa actcggcgcc
      661 tatagccgga ccgactttcg gcgcgacccg gcgggagaat tctggtgcct cgaagccaat
      721 tcgctgccgg gcatgaccgc aaccagtctc ttgccgcagg cggccaaggc ggccggcatc
      781 gtatttgcgg agctgctcga tcgcatctgc cgcggtgctc tcaaagcctg agcgcctact
      841 cggcagcgtc cgcctcggaa tggcacgtcg ccgcttgcga cgcggtgccg cgcccttcgc
      901 ccgcgcgcag ttccatgacg agccgcatgt tgtccgaacg gattcccaca ggaccctgca
      961 gacccgccgc atctcggccc agcgatcctt gccagacgat agcttcgtcg atgaagacgc
     1021 gcagatcgtc ggcgttcatc tgcgcccgca gagtgtgtgt ggcgccgggc ctaagatcga
     1081 caatcgccgc cgcgtgctgc ggtttgaggt tgcggtagcc gcgattgccg cactcagaac
     1141 tcgcactctg cccgggattc gtcttgacgg aaaccaccag ccgcggtgct ggctcgatgc
     1201 gccacatgac gtacaccagg ttgcacgcgt ccaaggcacg cagcttgaaa ccaaactggc
     1261 gtcgcagttc tcccgaagcg agcggcacgt cggtggccgt cggcccaacg taagtgacgc
     1321 gcgccgcgat cgactgttgc gtacttcgat tgaggtatgc gcgcatcttg gcggtgccca
     1381 cggacagccg cgtccccggc agctcttcaa ccgcaccttc ggtgacgcac agttcggcgc
     1441 gtgaaaccag cggcgatgac atggcgagac ggggtgacca gcagccgagg cagaacgcga
     1501 accaggctcc aagaaccgat agcggcgctc gtgttccggc cacgcggaat caaccgggtc
     1561 cgtttgccag cgtcgcaatg cgggcgaggc cggcttcgat cgattgtcgc acttcctcgt
     1621 tgagctgccg cgggtccttg ccggccggtt cgatcggctc gccaatgacg acgcggatcg
     1681 tgcccggctt ctttacgaag ccgcgccgcg gccagaacgt gccggcattg tgcgccaacg
     1741 gcaccacgga tttgccggag gcgattgcca gcagcgcgcc gctcacgccg aacttgcgct
     1801 tttcacccgc caccactcgg gtaccttcgg gaaaaataat gatccacagg ccgtccgcga
     1861 gacgtgactt gccttgttcg atcacctggt tgaccgcgcg gtgcccctcg ccgcggttga
     1921 tcgcgatcgg ccgcagcaac ttgagccccc agccaatgaa cggaatccac agtaattccc
     1981 gcttcagcac ccagacctgc ggcgggaaga tgagaaattg cgccacggtc tcccacgccg
     2041 aagtgtggtt gctcatgacg atgtgattcc cggagggaat ccgctcgcgt ccctcgacga
     2101 cgtacttgag cccgcacaag cgctcgagca cccaaaataa gatccgcgcc caggtgcggg
     2161 caatcgcgaa ctgcacccgg tacggcagcc agaaccccag cgccatcgcc gcgccgaaca
     2221 ggcacgccga cgccatcatg taagtggtga aaagcagcga ccggaacagc tgcacgagat
     2281 caattaaccc ggcaggcgcg acaggtcggc gaccccgccg agcagcagtt gcacgtcgcg
     2341 caacaatgcc aggcggttat tgcgtcgctc gatgttctca tccatcacca tgatgcgctc
     2401 gaaaaattca tcgaccggcg cccgcaagcc aaccaaagaa cgaagtccct gcgcatagcg
     2461 gcgctgccgg gttgcctcct gcagcggcgt gcgcagctcg gtcaacgcct ggtgcagctg
     2521 ccgctccgcg ctttccgtca gcgcggctgc atcgaccgac acgccgggtc ctagcgcagc
     2581 cttcttcagg atattcgcaa tgcgcttgtt gatggccgct aggataccgg cctcgggcaa
     2641 caacaggaac tccttcaagg cttgcaggcg tgtcgccgca tcgagcggtg agcccggacg
     2701 gttcgcgaac accgcgtcca gcatctcggc gctggtgccc ggctcttcca acagcatgcc
     2761 cttgagccgc tcgatgagaa aatcgtaaac ttcgtccacg acgccgggcc gcgccaccgg
     2821 ctgcgcagcg gcggcggttg tcagcagctc gaacaaatcc aaatccagcc gctcttcgag
     2881 catgatgcgc aacaatccca gggcggcgcg ccgcagtcca aaaggatcct tcgctcccgt
     2941 cggccgctgc tcgatggcga aaatcccggc caacgtatcg atcttgtcgg cgagtgcgat
     3001 gcactgaccg gtcttggttg tcggcaacga atcgccggca aacctcgggt ggtagtgctc
     3061 ttccagagcc agtgcgagct cctggggttc gccctgcgcc agtgcgtaat aacggcccat
     3121 cgtgccctgc agttccggaa attcgttcac catcgccgtc atcaaatcgg ccttggcgag
     3181 tagcgcggcg cgcgcgacgt aatctccggc gccgatgcgg ccgccgatca actgagccag
     3241 cgccttgacg cgcagtgtct tgtcggcgta gctgcccaac cgagtctgaa acgtcacgcc
     3301 gccgagcttt gccgcgtgct cgtcgagcga cagcttgcgg tcctgatccc agaaaaatgc
     3361 ggcatcgctc aatcgcgggc ggaccacgcg ctcgtttcct tgccgcacct tggcagggtc
     3421 gcggctctcg atattggcga cggtgatgaa ccaaccggac agccggccct cggccccttg
     3481 cacggcgaaa tagcgctgat gatcctgcac ggtcgcgatc acgacctcgc gcggcaggct
     3541 caagaatcgc tgctcgaact gcccggcgat cggcaccggc cattcgacca aggcggtcac
     3601 ctcatcgagc agcgcggctt cgatgagcgc cgtgccgccg ttgcagcggg ccgcggcttc
     3661 gatcacgccg tcgcgtatca gctcgcggcg cgcggcgaaa tcagcgacta ctttcgcgcg
     3721 gcgcaagcgc ccttcgtagc ttttggctga cttgagtgcg atcggcttcg gcgcgtgaaa
     3781 gcggtggccg aaggtcgtgc gtccggtcgg aaggccgagc acttcggcgg acaccaccgc
     3841 atcaccatac aaaagaacga ccgcatgaac cggccgcacg aattccgtgg aacggctgcc
     3901 ccagcgcata cgcttcgcga tcggcagcgt cgcgactgct tgattgacga tgtgaccgag
     3961 caggttcgcc gtcgcggtgc cgcgctcggt gccgcgaaaa accaaccagg ctcccttgtc
     4021 cgtctgcagc tgctcgagct cggcgagcgg cacgttgcaa ttcttggcaa acgcgattgc
     4081 ggcttgcgtc ggcgcgccgt gcgcgtcgaa gcacagcttg agcggcgggc cgcggcgttc
     4141 gac
//
LOCUS       DASWZT010000378         3221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_60978, whole genome shotgun sequence.
ACCESSION   DASWZT010000378 DASWZT010000000
VERSION     DASWZT010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60978"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VGE92_10650"
     CDS             complement(<1..849)
                     /locus_tag="VGE92_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314329.1"
                     /translation="MKVIRVLAFASAYAAALCVAAAYAADSRVAVLTGAQVIQILDDT
                     VDWYRTLGTQQQNATQPSDLLILFANRQTADKVVGLAFDIARANAELLSSEASASADA
                     SAAQTLTSQQNKLQAQSQSIQDEVTSGKQKLATAGKDKPVIEAKLPELQSELAMLSAR
                     KNLLDTMTEFVNSSDPKSAGANALKAQIDAIAATVPSAALNASTAPVTAAAPAATGAQ
                     PTATSAGNSGSAVATRSGIWDLATDVLRQRNKLDTISTVDRRTGALADTFRQIGAAPL
                     AQLKSLA"
     gene            complement(1039..1617)
                     /locus_tag="VGE92_10655"
     CDS             complement(1039..1617)
                     /locus_tag="VGE92_10655"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HEY1314330.1"
                     /translation="MQSKFRLALLSASALVLMAGTAYAGKYGDTIDLFRKAGESATFF
                     HRSYAYAVFPTVGEGGLGVGGALGKGHVYVHGRLVGDTTMGQVSVGFQAGGKAYSQII
                     FFEDKRALDNFESGTFEFTAGASAVAITAGANASVGTSGTSAGASGTENNARETGAYQ
                     KGMAVFTIAKGGLMYAATIAGQKFSFTPRGAS"
     gene            complement(1721..2107)
                     /locus_tag="VGE92_10660"
     CDS             complement(1721..2107)
                     /locus_tag="VGE92_10660"
                     /inference="COORDINATES: protein motif:HMM:NF022681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HEY1314331.1"
                     /translation="MPDLRTYDYAIVRVVPRVERGEFVNAGIILSCDIERILLSSIEL
                     DEGALLALHAAVDLELVRSTLASIPAVCAGGSAAGDLGKLSARERFHWLVAPRSTMVQ
                     TSPVHTGRCTDFDVAIEHLMDTMIRR"
     gene            complement(2085..2867)
                     /locus_tag="VGE92_10665"
     CDS             complement(2085..2867)
                     /locus_tag="VGE92_10665"
                     /inference="COORDINATES: protein motif:HMM:NF042851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HEY1314332.1"
                     /translation="MTLRTVAARRYVTPLREGGSLPAIIEADDDGLYVLKFRGAGQGV
                     KALIAELLAGEIGRYLGLAVPEIVFVELPAELARTEPDSEIRSLIGASAGLNIALDYL
                     PGSITFDPLVYEPDAQLASTIVWFDAYVCNVDRTVRNVNMLMWHRRLWLIDHGAALYF
                     HHAWTRSDRPASNPFALIKDHVLLKHASMLAEVASRMQTVLTVERLENLVESIPSLWL
                     EEDPGFATRESVRAAYLDFFVSRLQASDVFVEEAIRARSSHL"
BASE COUNT          589 a          996 c          982 g          654 t
ORIGIN      
        1 ggcgagagac ttgagttgtg ccagcggcgc ggcgccgatt tgccgaaacg tatcggccag
       61 cgcgccggtg cgccgatcga ccgtgctgat cgtgtcaagc ttgtttcgct gccgcagcac
      121 atcggtcgct agatcccaaa tgccggagcg ggtggcaacg gcgctgccgc tgttgcctgc
      181 ggacgtcgcg gttggttgcg cccccgtggc cgccggcgcg gccgccgtga ccggcgccgt
      241 ggaggcgttc agggccgcgc tcggtacggt ggcggcgatc gcgtcgatct gcgccttgag
      301 cgcgttggca ccggcagact tcggatcgct tgaattgacg aactccgtca tcgtgtcgag
      361 tagattcttg cgtgcgctca gcatggcgag ctcgctttgc agctcgggca atttcgcttc
      421 gatgacgggc ttgtctttgc cggcggtcgc gagtttttgt ttgccgctcg tcacctcgtc
      481 ttggatggat tggctttggg cctgcagttt gttctgttgg ctggtcagtg tctgcgctgc
      541 cgaggcatcg gcagacgcac tcgcctcgct gctgagcagt tcggcattgg cccgagcgat
      601 atcgaaagcc aggcccacga ctttatcggc cgtctgccga ttggcaaaca aaatcaacaa
      661 gtcgctcggc tgggtcgcgt tttgttgctg cgtgccgagc gtgcgatacc aatcgacggt
      721 gtcgtcgaga atttgtatga cctgagcgcc cgtcagcacc gccacgcgcg agtccgccgc
      781 atacgcggca gcgacgcaca gcgcggcggc gtacgccgag gcgaacgcca aaacgcgaat
      841 cactttcacc acaaaagcgc cttgttgcct cgaatcggcc gccagcatgc ggggctgtgc
      901 gtcgccaagt ccagtggtgc gctacgagcg cagtgcgtta gccattcgag cttatgctcg
      961 tatcgttatg atttaatgcc aaaatggcgg ggaattgcag gcactggacc tgcaagtccc
     1021 gcactttttg cctcgggctt aggatgcgcc ccgcggcgtg aaggagaact tttgtccggc
     1081 aatcgtggcc gcgtacatga gtccgccctt ggcgatggta aataccgcca tccccttctg
     1141 gtaggcgccg gtttctcgcg cattattttc ggtgccgctc gcaccggcgg acgtgccgct
     1201 ggtaccgacg ctcgcattgg caccggcggt gatcgcgacg gcgctggcgc ctgcggtaaa
     1261 ttcgaacgtg cccgactcga aattgtcgag tgcgcgcttg tcctcgaaga aaatgatttg
     1321 actgtaggcc ttaccgccgg cttggaagcc gacgctcacc tgacccatgg tggtatcgcc
     1381 caccagccgt ccgtgcacgt agacatgtcc tttgcccaac gccccaccga cgccgagacc
     1441 accctcgccg accgtcggga acactgcata cgcgtagctc ctgtggaaga acgttgcgct
     1501 ctcgccggct tttcggaaca agtcgatcgt atcgccatac ttgccggcgt acgccgtgcc
     1561 tgccatcagc acaagtgcgg aggcggaaag caaggccagt ctgaatttgc tctgcatatg
     1621 aatctctaat cctgccaatg ggccttgagc atcgactatc cggctcggcc gcggcagtgg
     1681 cggatgccca cttttcgagt aggcgtttac ctatagcaat tcagcgccgg atcatggtgt
     1741 ccatcaaatg ctcgatcgcg acatcgaaat ccgtgcagcg gccggtatgt acgggcgagg
     1801 tctgcaccat ggtgctgcgt ggcgcgacca gccaatgaaa tcgttcgcgc gccgacagtt
     1861 tgccgagatc gcccgccgcg gacccgcccg cgcataccgc cggaatgctc gccagggtgc
     1921 tgcgcaccag ctccaagtcc actgcggcgt gcagcgcaag cagcgcaccc tcatcgagct
     1981 cgatgctcga cagcaaaatc cgctcgatat cacaggacag gataatgccg gcgttgacga
     2041 actcgccgcg ctccacgcgc ggtactacgc gcactatcgc atagtcatag gtgcgaagat
     2101 cgggcacgga tcgcctcctc gacaaataca tcggacgctt gcagtctgga aacgaagaaa
     2161 tcgaggtagg ccgcgcgcac cgattctcgg gtcgcaaacc ccggatcctc ctcgagccag
     2221 agactcggaa tcgactcgac caggttttcg agtcgctcca ccgtcaacac cgtttgcatc
     2281 cggctggcga cctcggccag catgctcgca tgcttcagca atacgtgatc cttgatcaag
     2341 gcgaagggat tgcttgcggg tcgatcgctc cgggtccatg cgtggtgaaa ataaagtgcc
     2401 gcgccgtgat cgatcagcca gagccgtcgg tgccacatca gcatgttgac gttgcgcacc
     2461 gtgcggtcca cgttgcatac ataggcgtcg aaccagacga tggtcgacgc gagctgcgca
     2521 tcgggctcgt agaccaacgg atcaaaggta atcgatcccg gcaggtagtc caacgcgatg
     2581 ttcaagccgg cgctggcgcc gatgaggctg cggatttccg aatccggttc ggtgcgcgcg
     2641 agctccgcgg gcagttcgac gaaaacgatt tcgggtaccg caagtcctag gtaacggccg
     2701 atttccccgg ccagaagttc ggcaatcagc gctttgacgc cctgccccgc gccgcggaac
     2761 ttgaggacat agagtccgtc atcgtcggcc tcaatgatgg cggggagcga gcccccttcg
     2821 cgcagcggtg tgacgtagcg ccgggcagca acggttctca atgtcatccg gcaagcttac
     2881 ccggtcaacg ccctgttcgg cgggcacgct ttccacgaca aattttctgt cacggacgct
     2941 gaattgtcca ttacgattcg gcgtcaagac gttgtaatac ttacaaaaat gccgtgctta
     3001 taaccgtcgg gaagttccat atacgttctc gctattcgac gcgcgccgcg cctcggccta
     3061 gcgtgtcgcg gtcacgatcc attcatgcgg agttatcatg gcgcaactcg ccgaagcctc
     3121 tactcgactg cgggtgcgcc actccgcgtg gaaggaacta tcccgcaatg aagactggtg
     3181 ggcggtctgg atcggccttg cgctcgtggc catcgcggtg g
//
LOCUS       DASWZT010000379         2309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_89983, whole genome shotgun sequence.
ACCESSION   DASWZT010000379 DASWZT010000000
VERSION     DASWZT010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89983"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VGE92_10670"
     CDS             <1..402
                     /locus_tag="VGE92_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314333.1"
                     /translation="VRLPPAAAPANDAATRPAAAGAVRVEMAADTVDVASGDTMALVT
                     VRRKGNLHGVASFSWWTESGTAKPGTDFAPVTPRVGEIADGSASVSLSVPLSGVPRSQ
                     PKSFYVVIDHNDSGGAQLGAKTLTMVTLPAS"
     gene            505..1320
                     /locus_tag="VGE92_10675"
     CDS             505..1320
                     /locus_tag="VGE92_10675"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY1314334.1"
                     /translation="MSHRVLPASLIGLSLACAAGVAHADDSNNCKDLPGYHALQAALA
                     AATAAEKSGLNNQMWGTIVDRDGVVCAVAFSGVDRGAQWPGSRVISAQKANTANAFSL
                     DASSNSGGSGQPAGLSLSTANLYSAVNPGGSLFGLQASNPVNAEVAYHGPSSDYGTAD
                     DPMVGHKIGGVNVFGGGLALYGPGQKIIGAVGVSGDTSCADHDIAWRTRNNLKLDHLA
                     GVGGVSGDAAHPDNIIYDIKANPDGGTGISKGGFGHPTCINTGDQTALPPVRP"
     gene            complement(1379..>2309)
                     /locus_tag="VGE92_10680"
     CDS             complement(1379..>2309)
                     /locus_tag="VGE92_10680"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEY1314335.1"
                     /translation="PADASQLETASGLAALRLWRLAFGAPVVAPPLVRAHAQVRQLIC
                     AVLEVDELDADYFPRRPTLMPQLMQAVNDPGAAAEKLSRMIAHDPVLTADVLRLANSS
                     LYRASPAPIETIQRAIVVCGVEALRGILATAMLRPVFRASRSNFPRLPRMLWERTERA
                     ARAAELYAARTHPQDRFEAQLVVLLRALGPLVVYSAALDGYSRAPQLTPNPTLFVELI
                     GTMAPQISQRIARDWQASPRLLAALGKSGEGSLATALQVGELLGTLSLLESKNVITPH
                     ERLAIIQSAGLAADDLVDHIWTRLAGVPVKASA"
BASE COUNT          441 a          749 c          735 g          384 t
ORIGIN      
        1 gtcagactgc ccccggcggc ggcacccgcc aatgacgcag cgacccgccc cgccgcggca
       61 ggagcggtcc gcgtcgaaat ggcggctgat acggtcgatg ttgcgagtgg tgacaccatg
      121 gcgctcgtga cggtgcgccg caagggcaac ttgcatggcg tcgcgagctt ctcctggtgg
      181 accgaatccg gcactgcaaa acccggtacg gacttcgccc ccgtgacacc gcgagtcggc
      241 gagatcgccg acggcagcgc cagcgtcagc ttgagcgtcc ctctgtccgg cgtgccccgc
      301 agccaaccaa agagcttcta tgtggtcatc gaccataatg attcgggcgg tgcccaactc
      361 ggtgcgaaga ccctcaccat ggtgactttg cccgcgtcct gatcgacgag aatactgtta
      421 aaacgaacgc gtcgcattcg cgaatcgttc tagactgccg tgcgcttgcg catcaaatca
      481 caataaacct tcacggagtc aaccatgtcg cacagagttc ttcctgcttc actcattggc
      541 ctgtccctgg cgtgcgccgc cggagtcgca cacgccgacg acagcaacaa ttgcaaggac
      601 cttcccggat atcacgcatt gcaggcggcg ctcgcggctg ccaccgctgc cgaaaaaagc
      661 ggcctgaaca accagatgtg gggaacgatc gtcgatcgcg acggcgttgt ctgcgcggtt
      721 gcattttccg gagtcgaccg cggcgcccag tggccgggca gccgtgtgat ctcggcgcag
      781 aaggccaaca ctgcgaatgc cttcagtctg gatgcatcat cgaacagcgg cggttccggc
      841 cagcctgcgg gattgtccct gtcgacggcg aatttgtact cggcggtaaa tcccggcggc
      901 agtctgttcg gcctgcaggc gagcaacccc gtcaatgcgg aagttgcgta ccacggaccc
      961 tcctccgact atggcacggc ggatgatccg atggtcggcc acaagatcgg cggggtaaat
     1021 gtattcggcg gcggtcttgc cctgtatggc cccgggcaga agatcatcgg cgccgtggga
     1081 gtgagcggcg acacctcgtg cgcggatcac gacatcgcct ggcgaaccag aaacaatctg
     1141 aaactggatc atcttgcggg cgtcggtggt gtatcgggtg atgccgcgca cccagacaac
     1201 atcatctatg acatcaaggc gaaccccgac ggcggcacgg gaatcagcaa gggtggcttt
     1261 gggcatccga cctgcatcaa caccggcgat cagacggcgc taccgcccgt ccgaccgtaa
     1321 gttgaatcag cgaccgggct tcgaggcaac gccttgggtc cggtcgctgc gcttgggttc
     1381 aggcactcgc cttcaccggc actcccgcca gacgggtcca aatgtggtcc actaaatcat
     1441 cggcggcgag cccggcgctt tggatgatag cgagccgctc gtggggtgtg atcacatttt
     1501 tcgattccag tagcgacagc gtgccgagca actcgcccac ctgaagcgcg gtcgcgagac
     1561 tgccctcgcc cgatttgccc aatgccgcca acaggcgcgg cgaagcttgc caatcccgcg
     1621 cgatacgctg cgagatctgc ggcgccatgg tgccgatcag ctcgacgaac aacgtgggat
     1681 tgggcgtgag ctgcggcgcg cgcgagtagc cgtcgagcgc ggcgctgtaa accaccaacg
     1741 gtccgagcgc gcgcagcagc acgaccaatt gcgcttcgaa tcgatcttgc ggatgcgttc
     1801 gggcggcata cagttcggcg gcgcgagccg cgcgctcggt acgctcccac agcatgcgcg
     1861 gcaggcgcgg gaaattgcta cggctcgccc ggaaaaccgg gcgcagcatc gcggtcgcca
     1921 agattccgcg cagcgcctcc acgccgcaca cgacgatggc gcgctgtatg gtttctatcg
     1981 gcgccggcga ggcgcgatac aggctcgaat tcgccaggcg caacacgtcg gcagtgagaa
     2041 ccggatcgtg cgcgatcatg cgcgacagtt tctcggcggc agcgcccgga tcattgaccg
     2101 cctgcatcag ctgcggcatc aaggtgggac gtcgcggaaa ataatccgcg tcgagttcat
     2161 cgacctcgag cactgcacaa atcagctgcc gcacttgagc gtgtgcgcgc accagcggcg
     2221 gcgccaccac gggggcgccg aaggcgagac gccagagacg caaggccgcc aggccgctcg
     2281 cagtttcgag ctgcgaggcg tccgccggc
//
LOCUS       DASWZT010000380        10254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_46593, whole genome shotgun sequence.
ACCESSION   DASWZT010000380 DASWZT010000000
VERSION     DASWZT010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10254
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46593"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="VGE92_10685"
     CDS             complement(<1..1050)
                     /locus_tag="VGE92_10685"
                     /inference="COORDINATES: protein motif:HMM:NF037184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314336.1"
                     /translation="MFSLPGAPALSQFRIEQLLRSIRAQDPRVTALSSRWLHFIDESR
                     PLKATERSLLEKLLTYGSRAAVTAEHGQQLLVTPRVGTESPWSSKATDIAHVCGLQAV
                     RRLERATLYCIESQVPLEPLQLKHLAVLLHDRMTESVWIDCLDPTGLFHNAAARPLRI
                     VSLGKDGHAALGIANRDWGLALSSEEIDYLVRAFGELGRDPTDVELMMFAQANSEHCR
                     HKIFNADFIVDGVPMPASLFAMIRETYARNCVGVLSAYRDNAAVIEGGSATRFFPDPE
                     TQRYVGKREPVDILMKVETHNHPTAISPFPGAATGAGGEIRDEGATGIGAKPKAGLVG
                     FSVSNLKIPGMLQPWE"
     gene            complement(1071..1412)
                     /locus_tag="VGE92_10690"
     CDS             complement(1071..1412)
                     /locus_tag="VGE92_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1820 family protein"
                     /protein_id="HEY1314337.1"
                     /translation="MAASHIFKVLFVNQGKVYEIYARKVSHGSLFGFIEVEELVFGER
                     STVVVDPGEERIKAEFSDVKRTYLPMHSVLRIDEVKKQGVSKVVALEGSNVSQFPVPI
                     YTPGGDSNARK"
     gene            1498..2877
                     /gene="tagH"
                     /locus_tag="VGE92_10695"
     CDS             1498..2877
                     /gene="tagH"
                     /locus_tag="VGE92_10695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HEY1314338.1"
                     /translation="MALRLRVVSEHAIRLGERSTKVFGVHGGTIGRATNNEWSLPDPE
                     RYLSGKHARVDFRAGTYVLVDTSSNGTYLNGAQVPLGKFHEYALKDGDYVRLGQYELL
                     VSIDKSNDFPPEEGAIVAYDGQSPSAAAKKSTANDLGADLDLSELLEPSDQAAVDSGV
                     RPRDAYGQSLPRTAPPPPRASATPELESDDAGTPWHMLTRPLKVSAAKADSAATPTSA
                     PAPAPASAPLTAARSLSLTLHESGEFDAGLTAFCRGAGIDPRLIGAEVRGAVLQLAGQ
                     LLRESVLGLMDLNQSRNEFRNRFRIAPAPSEGGESPLNFSQGVDEVLMRLITTLSVRS
                     GSVVALRSNFRELKAQNTAAFSATRTAFEEFLGRVDPKELEERFDRASKRGVFGSQNR
                     AKYWELYGEMFAGLAQRPADGFPHLFTETFAKAFEAKFRTLVPPQRSTFGADRSDEKD
                     PGDQAVGDL"
     gene            2887..3558
                     /locus_tag="VGE92_10700"
     CDS             2887..3558
                     /locus_tag="VGE92_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY1314339.1"
                     /translation="MRLLLVEDDDIVADAIARGLAAADYRVHRVASAEAAQAALADEE
                     FALAIIDVGLPGADGLSLVRRLRSAGTTTPTLILTARCTLNDKVKALDLGADDFLSKP
                     FEPAELAARCRALMRRSSGALHGVIKLNRMSVDLLGKRLLIDDEEIELTAREWLLLEC
                     LVRRTGQVVSKERVQQAVANPDQDISANAVEVHVSRLRAKLGDAAIIRSLRGLGYRLE
                     EAKAS"
     gene            3555..4880
                     /locus_tag="VGE92_10705"
     CDS             3555..4880
                     /locus_tag="VGE92_10705"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY1314340.1"
                     /translation="MRSSISRALVLWLAVPLMLLALCGALVHYFNNVAPGVISSDRRL
                     KEAANAVVAHIHIKDGEFSLERTDAPPRLPAADSVLYAVRDTQGHLIGGDARLPPAPM
                     AITQLDHRNIRSVTTRFEMHGAAVLVTVADLHATAEPAARYGLMSTLLWDFVQLDITL
                     VLVWVGIQLGLRPVKRLREEIAARSPQDLRPLDESSVPREIAPAVVTLNRLFATLRSS
                     VQSQQQFIANTAHQLRTPITGLQAQLELLIAESAALPVKARLLTLQEGIRQLAHSANQ
                     LLALARADPAVNIATKNQSVALDALAGDVAARFFDRALQSHIDLGIDVTPASIAADPA
                     LIDDLLSNLVDNALKYTPAGGSVTVSAGQVNGKAHITVEDTGPGIPEADRERVRQRFF
                     RLPNSPGHGSGLGLAIVEEIAHLYGAAVTIGPGSAGKGARIVVRFPATV"
     gene            4953..5498
                     /locus_tag="VGE92_10710"
     CDS             4953..5498
                     /locus_tag="VGE92_10710"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEY1314341.1"
                     /translation="MIPQRQLGVTLVELMIVIAIATILMAIGVPSYRYVTKSNRVSGE
                     INALLGDLTFARYEAVKEGLPVEVCPAAATATTCTASTTWSSGWIVLSNAAATGGTAV
                     VLRRQLPFSNFNSTDTLTTVTAQSVTFNREGFATAGAIVNFSLHDATDTSSYTRCLVV
                     NVAGELATGLSGQTILTATCT"
     gene            5495..6103
                     /gene="pilV"
                     /locus_tag="VGE92_10715"
     CDS             5495..6103
                     /gene="pilV"
                     /locus_tag="VGE92_10715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEY1314342.1"
                     /translation="MKPLLRQRAARGFTLVEVMVALIVIAIGMLGIAKMQGLALSSTS
                     SSRTRALAAIEASSLASAMQANRAYWSAAGSLPGTITVVTSGGTATPTSTSATLQAAI
                     TQANGTLCSGTGSVLSCYCTTGNGAPCTTVALAGSDIFDWGQSLANFLPNATATVTCN
                     SANLPVDCTIQIIATENVVALNTQEAAAASTVSVNYTLNVVP"
     gene            6100..6867
                     /locus_tag="VGE92_10720"
     CDS             6100..6867
                     /locus_tag="VGE92_10720"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEY1314343.1"
                     /translation="MMPRRQHGYTLIEILVALLIAIFLLAGLFTILQNTRRTSSNQTG
                     LALLQDEQRMAMSMLNDVIQNAGYFDTNTYSTAQTAWPAATAVTATGTSLAAGQALTG
                     SHAAASPGDVLAVRYATAGGDGVINCNGATSLVAANYVNTFFVASTTAGGVTTYQLEC
                     SKDGITTDAVPLVQDVYNLQVWYGVSTTASTNNVDTYMTADQLTAANWSNVTSVRVTL
                     TFVNPVWKTAPAGQPQFVYFTRVIPIQGRTGVIPTNL"
     gene            6864..7481
                     /locus_tag="VGE92_10725"
     CDS             6864..7481
                     /locus_tag="VGE92_10725"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HEY1314344.1"
                     /translation="MKTARTKLPRGAQRGFVLITAILLLIVMTLLALGMFHSFGIQEL
                     VAGNVREKQRALQSAEAAEQYAEMWLTSQGNVLSNSVDCTTTNLVAYSATAVPYICMK
                     PVASIDDALNVWTVPWTISSVEVGFAFFPGNPASSPGDLQVSTSGGNNYYYQVPRFYI
                     GLVSNSATQALYRIDAWNYAGTTTTSAVVESNYVVTCTTCSVTGP"
     gene            7498..>10254
                     /locus_tag="VGE92_10730"
     CDS             7498..>10254
                     /locus_tag="VGE92_10730"
                     /inference="COORDINATES: protein motif:HMM:NF017388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HEY1314345.1"
                     /translation="MRMRTSTIVKAAGLLSVLFASGSFAQSPVTEDFTGASTTNSWYY
                     SGGACLTASSVAGTGTEFTSSVAGTAGTIPGCTAIRSNSDAYGGETLVGGYSGTLPDT
                     ATNGALRFTNGCISGSCTSGSGGGHNQFGAIISGNTFSSSAGVQVTFKTVTYRGDSQG
                     AGGDGADGITFFLQDGSVSPNIGQYGGSLAYTCSNQNGNYGYTGMVGAYIGLGIDEYG
                     NFLNGSNNTLGETGSTSTNGGAGDNTASGGLYQPNRIGLRGAGNIAYSWLSAKYPTYY
                     PTSASSSTQATAVQNTCRTGTLWNFSSSSSGTDTGTTSYNGTTLLDYPAIPNAYKVLS
                     GVTIANEYSNGGLTRGNATPILYKLKITTAGLLSLNYSFNGGAWQSVLQNQNITSSNG
                     ALPSTLRFGFAGSTGGSSNIHEVLCFKAASANTSSSSATTNQTQSSRVTSSTQAYFSY
                     YDPNDWTGRLTANAVTSDSSGNLSISTTATWDASCVLTTIPTGSSCTSTGVAGPTTGQ
                     NPVAGGTLSRVILSWNGSAGIPFEWSSLSAAQQSALDQGDATATANRLNYLRGVRTNE
                     IDASGNGLYRDRDSVLGDIVDSSPVAVGQPVLPYAITWRDKLHTSTTMPENASGAQTY
                     AQFISAEEQRENVVYVGANDGMVHGFRAGAFNSSNTYSNATNDGTEVLAYFPGSTLLS
                     AASSSPSTNCVSTVATQTTVQNIHGETPAITASSVTAPACTQAALDYSNPQYGHNFFV
                     DATPATGDLFYASGSGTGAWHTWLVGGLGAGGAALYALDVTTPANFSETTTPTNYPAA
                     VVVGEWNAANITCPSTTGCANNLGNTYGTPLIRRLHNGAWAVIFGNGFGSVTGDAGVY
                     IMSISPTTGAQTFYYLSTGVGTGTTTTPCSTSCDGIAYVSAVDLDGDHIVDYLYAGDL
                     KGNVW"
BASE COUNT         1886 a         3407 c         3117 g         1844 t
ORIGIN      
        1 ttcccagggt tgcagcatgc ccggaatctt caaattcgat accgagaagc cgaccaggcc
       61 cgctttgggc ttggcgccga ttccggtggc gccctcgtcg cgaatctccc cgcccgcccc
      121 ggtggctgcc cccgggaacg gcgagatcgc ggtcgggtga ttgtgcgttt cgaccttcat
      181 gagaatgtcg accggctcgc gcttgccgac gtagcgctgc gtctctggat ccgggaaaaa
      241 tcgcgtggcg ctgccgccct cgatgaccgc ggcgttgtcc cgataggcgg acaggacgcc
      301 gacgcagttg cgcgcatagg tttcgcgaat catcgcaaac agcgaggccg gcatcggcac
      361 gccatcgacg atgaaatcgg cgttgaaaat cttgtgccgg caatgctcgg aattcgcctg
      421 tgcgaacatc atgagttcga cgtcggtcgg gtcgcgcccc aactcgccga acgcccgcac
      481 caggtagtcg atctcctcgc tcgagagtgc gagtccccaa tcgcgattgg cgatgcccag
      541 cgccgcgtgc ccgtccttcc ccaagctcac gatgcgcagc ggccgcgcgg cggcattatg
      601 gaaaaggccc gtcgggtcga ggcaatcgat ccacacggac tcggtcatgc ggtcgtgcag
      661 caggacggcc aagtgtttga gttgcaacgg ttcgaggggc acttgcgact cgatgcagta
      721 cagggtggcg cgctcgaggc gcctgaccgc ctgcaggccg cagacgtgcg cgatgtcggt
      781 agccttgctc gaccagggcg attcggtccc gacgcgcggg gtgacgagca gctgctggcc
      841 gtgctcggcc gtgaccgccg cccgcgaacc gtaggtcaac agcttctcga gcagcgagcg
      901 ctcggtggcc ttaagcggcc gcgattcgtc gatgaagtgc agccagcggc tgctgagcgc
      961 cgtgacgcgc gggtcttgtg cccgtatgga acgcagcagc tgctcgatcc ggaactgcga
     1021 cagcgccggc gcgccgggga gactaaacat gaagcgtggc gggcatgcgg ctatttacgc
     1081 gcgttcgaat ccccgcctgg cgtgtaaatg ggcaccggga attgactgac gttggaaccc
     1141 tccaaagcca cgactttgct gacgccttgc ttcttgacct catcgatgcg cagcaccgag
     1201 tgcatgggca aataggtccg ctttacgtcg ctgaattcgg ccttgatcct ctcctcgccc
     1261 gggtccacga ccacggtcga acgctcaccg aagaccaatt cctccacttc gatgaagccg
     1321 aataggctgc cgtgactcac tttccgggcg tagatctcgt aaacctttcc ctgattgacg
     1381 aacagaactt taaaaatgtg actcgcggcc attcagcaaa ctccaaccgt tggtagtatt
     1441 tcaccatatt atacgggcac ttagcgcata cctgtcgccc cgtgggaggg attgaatatg
     1501 gccttgcgct tacgcgttgt cagcgaacat gcgatccggc tcggtgagcg atccaccaag
     1561 gtcttcggcg tgcatggcgg cactattggg cgtgcgacta acaatgagtg gagtttaccc
     1621 gacccggagc gttacctctc gggcaaacac gcgcgggtcg atttccgggc cgggacctac
     1681 gtgttggtcg acaccagctc caacggcacc tacctcaacg gcgctcaggt ccccctcggc
     1741 aagtttcatg aatatgcgct gaaggacggc gattacgtgc gcctcgggca atacgagttg
     1801 ctggtcagca tcgacaagag caacgacttt ccgcccgaag aaggcgccat cgtcgcctac
     1861 gacggccagt cgccgtcggc ggccgccaag aagtccacgg ccaacgattt gggcgccgat
     1921 ctcgacctga gcgagctgct cgagccgagc gatcaggcgg cggttgactc gggcgttcgg
     1981 ccgcgcgatg cctacggtca atcgctgccg agaaccgcac cgccgccgcc gcgcgcctcg
     2041 gcgacgcccg aattggaatc cgacgatgcc ggcacgccgt ggcacatgct gacgcggccg
     2101 ctgaaggtct cggccgcgaa agcggacagc gccgcgacgc cgacgtctgc gccggcgccc
     2161 gcgcctgcgt ccgcgccgct cactgcggcc cgcagcttga gtctcacgct gcacgagtcc
     2221 ggcgaattcg atgccggttt gacggccttt tgccgcggtg ccggtatcga tccgcgcctg
     2281 atcggcgcgg aggtccgcgg cgccgtgctg cagctcgccg gccagctgct gcgcgaatcg
     2341 gtgctcggct tgatggatct gaatcagagt cgcaatgaat ttcgcaatcg cttccgtatc
     2401 gcgcccgcgc cgagcgaggg cggcgaatcg ccgctgaatt tctcgcaggg cgtcgatgag
     2461 gtgctgatgc ggctgatcac cacattgtcc gtacgcagcg gctccgttgt agccctacgc
     2521 agcaactttc gcgaattgaa ggcgcagaac acggccgcat tttccgccac gcgcacggct
     2581 ttcgaggagt ttctcgggcg cgtcgacccc aaggagctgg aggagcgctt cgatcgggcg
     2641 agcaaacgcg gcgtcttcgg ctctcagaac cgagccaagt attgggaact ctacggcgaa
     2701 atgttcgccg gcctcgcgca gcgtcccgcc gacgggtttc cgcatctgtt caccgagact
     2761 ttcgccaagg ccttcgaggc gaagttccgc accctggtgc ccccgcaacg cagcaccttc
     2821 ggcgctgatc ggtccgacga gaaggatccc ggcgatcaag ccgtcggcga tctgtaatat
     2881 gagcccgtgc ggctactcct ggtagaagac gatgacatcg tggcggacgc gatcgcgcgc
     2941 ggtttggccg ccgccgacta tcgcgtgcac cgggttgcca gcgccgaggc ggcccaggcc
     3001 gcgttggccg acgaggaatt cgcgctcgcc atcatcgatg tggggctgcc cggcgcggac
     3061 ggcttgagtc tggtgcggcg cttgcgcagc gccggcacga ccacgcccac attgattctg
     3121 accgcgcgtt gcacgctgaa cgacaaggtc aaggcgctcg acctcggcgc ggatgatttt
     3181 ttgagcaaac ccttcgaacc cgccgagctc gccgcgcgct gccgggcgct gatgcgccgc
     3241 tccagcggcg cgctgcacgg ggtcatcaag ctcaaccgaa tgagcgtcga tctgttgggc
     3301 aagcggctcc tcatcgacga tgaggaaatc gagttgaccg cgcgcgagtg gctgctgctc
     3361 gaatgcctgg tacgccgcac cggccaagtc gtctccaagg aacgcgtgca gcaggccgtg
     3421 gccaatccgg accaggatat ttccgcgaac gccgtcgagg tgcatgtgtc gcggttgcgc
     3481 gcgaaattgg gcgatgccgc cattatccgc agcttgcgcg gtttgggata tcgcctggaa
     3541 gaagcgaagg cttcgtgagg tcctcgatca gccgggctct ggtgctgtgg ttggcggtcc
     3601 cgctgatgtt gctggcgctg tgcggcgccc tggtccatta cttcaacaat gtcgcgcccg
     3661 gcgtgatcag cagcgaccgg cgcctcaagg aagccgccaa tgccgtcgtc gcgcacatac
     3721 acatcaagga cggtgagttt tcgctggagc gcaccgacgc gccgccgcgc ctgccggcgg
     3781 ccgattcggt gctgtacgca gtgcgcgaca cgcaggggca tttgattggc ggcgatgcac
     3841 ggctgccgcc ggcgccgatg gccattacac agctcgatca ccgcaacatc cgcagcgtca
     3901 cgacgcgatt cgagatgcac ggcgctgccg tgctcgtgac cgtggccgac ctgcacgcga
     3961 ctgccgaacc cgcggctcgt tacggcttga tgagcacgtt gctctgggac ttcgtgcagc
     4021 tcgatatcac cttggtgctg gtgtgggtgg gtattcaatt gggcttgcgg ccggtgaagc
     4081 ggctgcgtga agaaatcgcg gcgcgttcgc cccaggactt gcgcccgctc gatgagtcct
     4141 cggtgccgcg cgagatcgcg cccgcggtcg tcacgctcaa tcgcctgttc gccacgctgc
     4201 gttcatccgt gcaatcgcag cagcaattca tcgccaacac cgcacatcag cttcgcaccc
     4261 cgatcaccgg cctgcaagcg cagctcgaat tgctgatcgc ggagtccgcg gccttgccgg
     4321 tcaaggcgcg cttgctgacc ttgcaagagg ggatccggca gctcgcgcat tctgccaatc
     4381 agctgctggc cctggcgcgc gcggacccgg cggtgaacat cgcgaccaag aatcagagcg
     4441 tggcgctcga tgctctcgcg ggcgatgtcg ccgcgcgctt tttcgaccgg gcactgcaat
     4501 cccatatcga cctagggatc gatgtcacgc ccgcgtcgat cgcagcggac cccgccctca
     4561 tcgacgacct gttgagcaac ttggtcgaca acgccctcaa atacacaccg gcgggcggca
     4621 gcgttaccgt gagcgcggga caagtcaacg gcaaggcgca tatcacggtg gaggacaccg
     4681 gcccagggat tcccgaggcc gatcgcgagc gggtccggca aaggttcttt cgcttgccga
     4741 attcgcccgg ccacggcagc ggcttggggc tcgccatcgt cgaggagatc gcgcacctgt
     4801 atggcgcggc cgtgacgatc ggcccaggtt cagctggcaa gggcgcccga atcgtcgtac
     4861 ggttccctgc cacagtgtga tcgccgtcac ggcttgcatt caaggtgagt ccgagacagc
     4921 ttcgtgcaag gttgccgcag taaggtgtcg ccatgattcc acaacgccaa ttgggcgtaa
     4981 cgctggtcga gttgatgatc gtgatcgcca tcgcgaccat cctcatggcc atcggcgtgc
     5041 cctcgtaccg atacgtcacc aagtctaacc gcgtcagcgg cgagatcaac gcgctgttgg
     5101 gcgacctgac gtttgcccgc tacgaagcgg tcaaggaggg tttgccggtc gaggtttgcc
     5161 cggccgccgc caccgcgacg acctgtaccg cctcaaccac ctggagcagc ggctggatcg
     5221 tgctgtcgaa tgcggcggcg accggcggca ccgccgtcgt cctgcggcgc cagctgccct
     5281 tcagcaactt caacagcacc gacacgctca cgaccgtaac ggcgcaatcc gtgaccttca
     5341 atcgagaagg cttcgcgacc gcgggtgcca tcgtcaattt ctcgctgcat gacgccaccg
     5401 acacctcgtc gtacacgcgt tgcctcgtcg tcaacgtggc cggcgagctc gcgaccggac
     5461 tctccggcca gacgattttg acggcgacgt gcacatgaag ccgctccttc gacagcgcgc
     5521 ggcgcgcggc ttcacattgg tcgaagtcat ggtggcgctc atcgtcattg ccatcggcat
     5581 gctcggcatc gccaaaatgc agggtctggc gctatcgagt accagctcat cgcgtacgcg
     5641 cgcgctcgcg gccatcgagg catcgagcct cgcctccgcg atgcaggcga atcgcgccta
     5701 ttggtcggcg gcgggcagcc tcccgggcac gattaccgtc gtcacctccg gcggcaccgc
     5761 aacgccgacg tccacgtcgg ccaccctgca ggcggccatc acgcaggcaa atggcaccct
     5821 gtgttccggt acgggctcgg ttctgtcctg ctattgcacc accggcaacg gcgcaccctg
     5881 caccactgtc gctctcgcgg gcagcgatat cttcgactgg ggtcaaagtc tggcgaactt
     5941 cttgcccaat gccacggcca cggttacttg caatagcgcc aacctgccgg tcgattgcac
     6001 gattcagatc atcgcaaccg agaatgtggt tgcgctcaac acgcaggaag ccgctgccgc
     6061 ctcgaccgtc tcggtcaact acaccctgaa cgtggtccca tgatgcctcg acgccaacac
     6121 ggctacacgc tcatcgaaat cctggtggcg ttgttgatcg cgatatttct gctcgcgggg
     6181 ttgttcacca tcctgcagaa tacgcggcgc accagctcga accaaaccgg gttggcgctg
     6241 ctgcaggacg aacagcgcat ggcgatgtcg atgctcaacg acgtcattca aaatgccggc
     6301 tacttcgaca ccaacaccta ctcgacggcg cagacagcgt ggcccgccgc gacggcggtc
     6361 accgcgaccg gtacgagcct cgccgcggga caggcgttga ccggttcgca tgcggccgct
     6421 tctccgggcg atgtgctcgc cgtgcgctat gccaccgccg gcggcgacgg ggtaatcaac
     6481 tgcaatggcg ccacgagtct tgtcgccgcc aactacgtca atacgttctt cgtcgcctcg
     6541 accaccgccg gcggcgtgac gacctatcag ctggaatgtt cgaaagacgg catcaccacc
     6601 gatgccgtgc cgctcgtcca ggacgtctac aatctccagg tgtggtacgg cgtcagcacc
     6661 acggccagca ccaacaatgt cgatacttat atgacggccg atcagctcac cgccgccaat
     6721 tggtcgaatg tcacctccgt gcgggtcacc ctaaccttcg tcaatccggt gtggaagacg
     6781 gccccggcgg gacagcctca attcgtctat ttcacgcgag tgattccgat tcagggccgc
     6841 accggcgtca ttccgaccaa tctatgaaaa ccgcacgcac taaacttcct cgcggcgcgc
     6901 aacgcggctt cgtgctgatc acggcgatct tgctgctgat cgtcatgacg ctcctggccc
     6961 tggggatgtt ccacagcttc ggcattcagg aattggtcgc cggcaacgtg cgcgaaaagc
     7021 agcgcgcgct gcagagcgcc gaggccgccg aacaatacgc ggagatgtgg ctgacctcgc
     7081 agggcaacgt gctctcgaac agcgtcgact gcacgacgac caatttagtc gcttactccg
     7141 cgacggcagt accctatatt tgcatgaagc cggtggcttc catcgacgat gcgctcaacg
     7201 tgtggaccgt gccgtggacg atcagcagcg tcgaagtggg attcgcgttc tttccgggca
     7261 atcccgccag cagtcccggc gacttgcagg tgtcgaccag cggcggcaac aactactact
     7321 accaagtgcc gcgattttat atcggcctgg tctcgaattc ggcgacccag gcgttgtacc
     7381 ggatcgatgc ctggaattat gccggcacga ccactacgtc tgccgtcgtt gaaagcaatt
     7441 acgtggtgac ctgcactact tgcagtgtca ccgggccgta atctagaggg tgatctcatg
     7501 agaatgcgca catcgaccat tgtcaaggca gcggggctcc tcagcgtgct gttcgcctcc
     7561 ggcagcttcg cccaaagccc ggtcactgaa gacttcacgg gagcgtccac gaccaactcg
     7621 tggtactaca gcggcggcgc ctgcctgacc gcgagcagcg tggccggcac cggcaccgag
     7681 ttcacttcaa gcgtcgcggg cactgccggc acgattccgg gctgcacggc gatcaggtcg
     7741 aattccgacg cctatggcgg cgagacgctg gtcggcggct attcgggcac gcttcccgac
     7801 accgcgacca acggcgcgct gcgcttcacc aacggttgca tcagtggcag ctgcacctcc
     7861 ggctccggcg gcggccacaa tcagttcggc gcgatcattt ccggcaacac tttctcgagc
     7921 agcgccggcg tgcaagtgac cttcaagacc gtgacctacc gcggcgactc gcaaggcgcg
     7981 ggcggcgacg gtgccgacgg tatcaccttc tttctgcagg atggcagcgt ttcgcccaac
     8041 atcgggcagt acggcggcag tcttgcatac acctgttcca accagaacgg caactacggc
     8101 tacacgggta tggtgggcgc ctacatcggc ctcggcattg acgagtacgg caacttttta
     8161 aacggctcga acaacacgct cggcgaaacg ggttcgacca gcaccaacgg cggcgcgggc
     8221 gataacacgg caagcggcgg cctgtaccaa ccgaatcgta tcggtctgcg cggcgcgggc
     8281 aacattgcct attcgtggct cagcgcaaaa tatcccacct attacccgac cagcgccagt
     8341 tccagcacgc aggcgaccgc ggtgcagaat acctgccgca ccggcacctt gtggaacttt
     8401 tcgagttcat cgagcgggac cgataccgga accacctcgt acaacggaac gacattgctc
     8461 gactatccgg cgatcccgaa tgcctacaag gtgttgtccg gcgtcaccat cgccaatgaa
     8521 tacagcaacg gcggcctgac gcgcggcaac gccacgccga tcctgtacaa actcaagatc
     8581 accaccgccg gtttgctgag cttgaactac agtttcaacg gcggcgcctg gcaatcggtg
     8641 ctccagaatc agaacatcac gagctcgaac ggtgccctcc cctcgacctt gcgtttcggc
     8701 ttcgccggtt ccaccggcgg cagctcaaac attcacgaag tgctgtgttt caaggcggcc
     8761 tcggccaaca catcgagcag ttccgccacc accaaccaga cgcagtcctc gagggtcacg
     8821 agcagcacgc aggcctactt ttcctactat gatccgaacg actggaccgg acggctgacc
     8881 gccaacgccg taacttccga ctcctccggc aatctctcga tctcgaccac cgccacctgg
     8941 gatgcgtcct gcgtcctgac cacgattccg accggctcga gctgcacgag caccggcgtc
     9001 gcgggtccga ccaccgggca gaacccggtt gccggaggaa cgctcagccg cgtgatcttg
     9061 agttggaacg gcagcgccgg cattcccttt gagtggagca gcctgagcgc cgcccagcaa
     9121 tcggccttgg accagggcga tgccacggca accgccaatc gcctcaatta tctgcgcggc
     9181 gtgcgcacca acgaaatcga tgccagcggc aacggactgt accgcgatcg cgattcggtg
     9241 ctcggcgata tcgtggactc gagtcccgtc gcggtcgggc agccggtgtt gccgtatgcg
     9301 atcacctgga gagacaagct ccataccagc acgaccatgc ccgagaatgc ctcgggcgcc
     9361 caaacctatg cgcaattcat ctcggccgag gagcagcgcg agaatgtcgt ctacgtgggc
     9421 gccaacgacg gcatggttca cggctttcgc gccggtgcat tcaacagcag caacacctac
     9481 agcaacgcca ccaacgacgg caccgaggtg ctggcgtatt tcccgggctc gacgctgttg
     9541 agtgccgcct cctccagccc ctccacgaac tgcgtcagca ccgtagccac ccagaccacg
     9601 gtgcagaaca ttcatggcga aactccggct attaccgcga gttccgtgac tgcgccggcc
     9661 tgcacgcaag cggctctcga ttactccaac ccgcagtacg gccacaactt cttcgtcgac
     9721 gcgacaccgg ccaccggtga cttgttctat gcctcgggca gcggaacggg cgcatggcac
     9781 acgtggctgg tcggcggcct gggtgccggc ggcgccgcct tgtacgcgct cgatgtcacg
     9841 acgcccgcca acttcagcga gaccacgaca cccaccaact atccggccgc cgtcgtggtg
     9901 ggtgaatgga acgccgcgaa catcacctgc ccgagtacca cgggctgcgc caacaacctg
     9961 ggcaacacct acggcacgcc gctgatccgc cgcttgcaca atggtgcgtg ggccgtgatt
    10021 ttcggcaatg gcttcggcag cgtcaccggc gatgccggtg tctatatcat gtcgatcagc
    10081 ccgaccacgg gcgcgcagac cttctattac ttgagcaccg gcgtcggcac cggcaccacc
    10141 acgaccccct gcagcacgag ttgcgatggc attgcgtacg tgtccgcggt ggatctggac
    10201 ggcgatcata tcgtcgacta tctgtatgcc ggcgacctca aaggcaatgt ctgg
//
LOCUS       DASWZT010000381         3600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_27171, whole genome shotgun sequence.
ACCESSION   DASWZT010000381 DASWZT010000000
VERSION     DASWZT010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27171"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1866
                     /locus_tag="VGE92_10735"
     CDS             <1..1866
                     /locus_tag="VGE92_10735"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1314346.1"
                     /translation="AGRFVELERSSRELLTLHPSVGVLWQLLAVALTRQGKDALDALT
                     QAAGLMPDDAGAQNNLGNALARLGQLDAAVTHYRRALELNCEFAEAHNNLGHALLDLG
                     RHEEAQIACRRAVTLKPLFAEAHDNLGSAQLASGQIDAALSSYRRALQIEPEFFEAHS
                     NLGNALLELGHLDAALASYRRALAIAPDFAEAHNNLGNALRNLGDLSEAEASYRRAVQ
                     INPRFAAAYCNLGIALRLQGRTALAQSSCRRALEIEPRSATALTVLAESSADRGEFAD
                     AEALYRRAIAIEPESPEAWAALARLRTMTRGDADWLAQAQRIVARPLPPRREIGLRYA
                     IGKYFDDVRDFEAAFSNFRRANELTKSRRAPYDAARLTQTVERIIGTYDQAWMHRPRD
                     PAIESARPIFIVGMLRSGTTLAEQILASHPAVFGAGELKFWSAAAAARHAMPAGLAAD
                     YLRLLHALSADALRVTDKMPTNFAHLGLIHAALPNARIIHMQRNALDTCLSIYFQHFE
                     TTVSYANDLQDLAHYYGEYRRVMQHWRSTIPGNSILDVPYEGLVADHEAWSRKMLEFV
                     GLPWDARCLDFHRTARTVITASKWQVRQKLQNTSVGRWRNYQRFIGPLLSMALDQ"
     gene            complement(2038..3081)
                     /locus_tag="VGE92_10740"
     CDS             complement(2038..3081)
                     /locus_tag="VGE92_10740"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY1314347.1"
                     /translation="MAINLTPASVTIQEVADLAKVSPKTVSRVINNEPRVRSDTRARI
                     LDAIERLNYRPNLNARGLASNRSFLIGLFCERPGDYLSEFQAGAVERCRESSLHLMVE
                     PWDVDSPDIDKQIDTLLGQLRLEGVILLPPLSDHPVVLSKLHEASIPIVRIAPKHNLS
                     ATPSIGIDDYAAARQVTAHLAGLGHRRIGFILGRPEHGATEQRYLGFVDEMRARQIPV
                     ESDLVQTGNFVFNDGLICAERMLRAASPPTAIFASNDDMAAAVISMARKFGLDLPGQL
                     SVTGFDDAPVATMIWPELTTVRQPVAAMARIAADLIIQLEPRRNGWPERIPRHLLDHE
                     LVIRNSTARLTRR"
     gene            complement(3147..>3600)
                     /locus_tag="VGE92_10745"
     CDS             complement(3147..>3600)
                     /locus_tag="VGE92_10745"
                     /inference="COORDINATES: protein motif:HMM:NF037461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEY1314348.1"
                     /translation="QESPQGAILAASDPDGSAIVWHGTRSGWFLISGRARDLRPLLAQ
                     GSSLELRYRVDRLPEHSVKLGLRCTESNCGSEVGAMRDVTGVFKNGAAGSWQTLSIPL
                     SCFAAKGASLASVEVPLAIETSGRFGLTISEVNLAQKTAPEPTCPGAI"
BASE COUNT          671 a         1151 c         1114 g          664 t
ORIGIN      
        1 gcggggcgct tcgtcgagtt ggaacgcagc agccgcgaac tgctgaccct tcatccaagc
       61 gtcggtgtcc tgtggcagct tctcgcggtg gcgctcacgc gccagggcaa agacgccctc
      121 gacgcgctga cccaggcggc cggattgatg ccggacgatg ccggggcgca aaacaatcta
      181 ggcaatgccc tcgcaagact cgggcagctc gacgccgcgg tgacgcatta ccggcgcgca
      241 ctcgagctca actgcgagtt tgccgaggcg cataataatt taggtcacgc gctgctcgat
      301 ctcggccgcc acgaggaagc gcaaatcgcc tgccgccgcg ccgtgactct caaacccctg
      361 ttcgccgagg cgcacgacaa cttgggcagc gcacaactcg cgtccggcca gatcgacgct
      421 gcgttgtcga gctaccgccg cgcgctgcaa atcgagcctg aatttttcga ggcgcacagc
      481 aatttgggca acgcgctgct cgaactcggt cacctcgatg ccgcactggc gagttaccgc
      541 cgcgcgctgg cgatcgctcc cgattttgcc gaagcgcaca acaatctcgg caacgccttg
      601 cgaaaccttg gcgacctgag cgaggccgaa gcgagttatc gtcgagccgt gcagatcaat
      661 ccgcggttcg ccgcagccta ctgcaacctc ggaatcgctc tcagattgca ggggcgcacg
      721 gccctcgcgc agtcaagttg ccggcgtgcg ctggaaattg agcctcgctc ggcaacggct
      781 ttgaccgtac tggccgagtc gagcgccgac cggggagaat ttgccgacgc cgaggcgctc
      841 taccggcgtg ccatcgccat cgagccggag tcgccggagg cctgggccgc cctcgcccgc
      901 ttgcgcacga tgacccgcgg cgatgcagac tggttggcac aagcgcagcg gatcgtggcg
      961 cggccgctgc cgccgcgccg ggagatcggt ttgcgttacg cgatcggcaa gtacttcgac
     1021 gacgtgcggg atttcgaagc ggcgttcagc aacttccggc gcgccaacga gctcacgaag
     1081 tcacggcgcg cgccgtacga cgccgcgcgg ctgacccaga ccgtcgagcg catcatcggc
     1141 acctacgatc aggcgtggat gcatcgcccg agagaccctg cgatcgagtc ggcgcgcccg
     1201 atattcatcg tcggcatgct gcgctccggc accacgttgg ccgagcaaat actggcttct
     1261 cacccggcgg ttttcggcgc cggggagctc aagttctgga gcgccgcagc ggcagcgcgc
     1321 cacgctatgc cggccgggct cgccgcggac tatcttcggc ttctgcacgc actgtcggcg
     1381 gacgcgctgc gggtgaccga caagatgccg acgaacttcg cgcatctagg cttgatacac
     1441 gccgcgctgc ccaacgcgcg catcatccac atgcagcgca atgcgctcga cacctgtctg
     1501 tccatctact ttcaacattt tgaaaccacg gtctcctacg ccaacgattt gcaggatttg
     1561 gcgcattact atggcgagta tcgacgcgtc atgcagcatt ggcggtcgac catacccggc
     1621 aactcaatct tggatgtccc ctatgaaggg ctggttgcag accatgaggc gtggagtcgc
     1681 aagatgctcg aattcgtcgg gctgccgtgg gatgcgcgct gcctggattt tcaccgtacc
     1741 gcgcgcaccg tgatcaccgc cagcaagtgg caggtgcgcc agaaactcca gaacacatcg
     1801 gtgggtcgct ggcgcaacta ccaacgattc atcgggccgt tactctcgat ggcattggat
     1861 caatagggtt cgcggttgcg gtggctgtat cccgcgcggc ctcgtccgag tgccaatgcc
     1921 gctgactccg ttgggcgcat ggccgatcgt aaggcgtcgg atagctcgaa ccggtgacgg
     1981 actttgttgc acctacgtcg attctcttgg gcactgaatt ttcctcggat acgcaggtca
     2041 tcggcgcgtg aggcgcgccg tggagttgcg aatcaccaat tcatggtcaa gcagatggcg
     2101 cgggatgcgc tctggccagc cattgcgcct cggctcgagt tgaatgatca agtctgccgc
     2161 gattcttgcc attgccgcta ccggctgccg cactgtcgtg agttcgggcc aaatcatggt
     2221 ggccacgggc gcgtcgtcga aacccgtcac cgacagttgg cccggcaaat ccagaccgaa
     2281 tttacgcgcc atcgagatca ccgccgccgc catgtcgtcg ttgctcgcga aaatcgcggt
     2341 cggcgggctg gcggcgcgca gcattcgctc cgcgcaaatc aagccgtcgt tgaagacgaa
     2401 gttcccggtt tgcacgaggt ccgactccac cgggatttgt cgcgcccgca tctcatcgac
     2461 aaaaccgaga tagcgctgtt cggtggcacc gtgctcgggc cggccgagaa taaagccgat
     2521 gcggcgatgt ccgagacccg caagatgggc ggtcacctgc cgtgcggccg cgtaatcgtc
     2581 gatgccgatc gatggcgtgg cgctcaggtt atgttttggc gcgattcgca cgatggggat
     2641 ggacgcctcg tgaagtttgc tgagtaccac ggggtgatcg ctcaggggag gcagcagaat
     2701 gacgccctcg aggcgcaact gtcccagcaa ggtatcgatc tgtttgtcga tatccgggct
     2761 gtccacgtcc caaggctcga ccatcaagtg caagctcgac tcgcgacaac gctcgaccgc
     2821 gccagcctgg aactcgctta agtaatctcc cggcctttca cagaacaagc cgatgagaaa
     2881 cgaacgattg ctcgccaacc cccgagcgtt gaggttcgga cggtaattga gccgctcgat
     2941 ggcatcgaga atgcgcgcgc gagtgtcgga acgaacccgc ggctcattat taatgacccg
     3001 cgagaccgtc tttggcgaga ccttggcaag atccgcaact tcttggattg tgaccgatgc
     3061 gggagttagg ttgatggcca atgcgaaaac ctcgggcgta ctggccctag tttaacggct
     3121 ggcgcagctc gcgcgccgca aaacgctcag atcgcacccg ggcaggttgg ttcaggcgca
     3181 gttttttgcg ccaagttaac ttctgatatg gtcaatccga agcggcccga ggtttctatg
     3241 gcaagaggga cctccacgct tgcgaggctc gcccctttgg ccgcaaagca cgacagcgga
     3301 atcgacagcg tttgccagct gcctgccgct ccattcttga aaacaccggt cacatcccgc
     3361 attgccccga cttcactccc gcaattggac tcggtgcagc gcaggcccaa cttgacgctg
     3421 tgttcgggaa gccggtcgac acgataacgc agttcgagag agcttccttg ggccagcagc
     3481 gggcgcagat cccgggcgcg cccggaaatc aagaaccagc cgctgcgcgt cccgtgccag
     3541 acgatggcgc tgccatcggg gtccgaggcg gcgaggatcg ctccctgcgg actctcctgc
//
LOCUS       DASWZT010000382         1677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_46635, whole genome shotgun sequence.
ACCESSION   DASWZT010000382 DASWZT010000000
VERSION     DASWZT010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46635"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..>1677
                     /locus_tag="VGE92_10750"
     CDS             12..>1677
                     /locus_tag="VGE92_10750"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HEY1314349.1"
                     /translation="MSTRDPLVVFTPSGKRGNFPVGTPLLQAARSLGVDIDSVCGGRG
                     LCGRCQVLVAEGEFAKHGVSSSSASLSPFSEPEMRFSRRAPLAAGRRLSCSARVAADV
                     VIDVPESSQVHRQVVRKEADARAIELNPVVRLHYVQVQEPDMHEPSGDLRRLFQALET
                     EWNLKDLTCDLSVLQTLQPTLRAGAWQVTVAVHAAKQVIAVWAGLHELAYGLAVDVGS
                     TTIAAHLCDLTSGEVVASAGVMNPQIRFGEDLMSRVSYSMMNPGGAALMTAAVREALC
                     GLALDVARQAGVTHEDILEATLVGNPIMHHLLLGIDPVELGGAPFALAVDHSLTLRAN
                     VIDFKLNRNARVYVLPCIAGHVGADAAGMVLAERPDLSGDMTLLVDVGTNAEIVLGNS
                     TRLVACSSPTGPAFEGAQISCGQRAAPGAIERVRIDRSTLEPRFKVIGSDLWSDEPGF
                     AAATAATGITGVCGSGIIEVIAEMYLAGIINQDGVLDGRLAARSPRIVANGRTFAYVL
                     QQGTVDMKITQNDVRAIQLAKAALYAGIALLMERLGIDHVDRIRLAG"
BASE COUNT          269 a          528 c          581 g          299 t
ORIGIN      
        1 gaacgatacc gatgtccacg cgcgacccgc tcgtcgtatt cacgccttcg ggcaagcgcg
       61 gtaattttcc cgttgggacg ccgctgctgc aggcggcacg cagtctcggc gtcgacatcg
      121 actcggtgtg cggcggccgc ggtctgtgcg ggcgctgcca ggtgctggtt gcggagggcg
      181 agttcgccaa gcatggtgtt tcctcatcga gcgcgagcct ctcgccgttc agcgagccgg
      241 aaatgcgctt ctcgcgccgc gccccgctgg cggcgggccg gcgcctgtcg tgttcggcgc
      301 gggtcgcagc ggatgtggtc atcgacgtgc cggagagcag ccaggttcac cggcaagtcg
      361 tgcgcaagga agcagacgcg cgcgccatcg aactcaatcc ggtggtacgt ctgcactacg
      421 tgcaggtgca ggagccggac atgcacgagc cctccggcga cctcaggcgg ctgttccagg
      481 cgctcgaaac ggaatggaat ctgaaggatc tcacctgcga tctatcggtg ctgcagaccc
      541 tgcagccgac attgcgcgcc ggtgcctggc aagtcaccgt ggcggttcat gccgcgaaac
      601 aggtcatcgc ggtctgggcc ggactgcacg aacttgcata tggccttgcg gtggatgtcg
      661 gctcgacgac catcgccgcg cacctctgcg atctgaccag cggcgaggtt gtggcctcgg
      721 cgggggtcat gaatccgcag attcgtttcg gcgaggacct gatgagccgc gtctcctatt
      781 cgatgatgaa cccagggggc gcggcgttga tgacggcggc ggtgcgcgaa gcgctgtgcg
      841 gtctggcgct cgatgtggcg cggcaggcgg gagtgacgca cgaggacatt ctcgaggcga
      901 cgctggtggg caaccccatc atgcatcatc tgctgctcgg catcgacccg gtggaactcg
      961 gcggtgcccc atttgcgctc gcggtcgatc actcgctcac gctgcgtgcg aatgtcatcg
     1021 acttcaagtt gaatcgcaat gcgcgtgtct acgtgctgcc gtgtatcgcc gggcacgtgg
     1081 gcgccgatgc ggcggggatg gtgctcgccg agcgtccgga tctatccggc gacatgacct
     1141 tgctggtcga cgtcggcacc aacgcggaaa ttgtgctggg gaatagcacg cggctggtcg
     1201 catgctcgag ccctacgggc cccgcgttcg aaggcgccca gatcagctgc gggcagcgcg
     1261 cggcgccggg agccatcgag cgcgtgcgca tcgatcgcag cacactcgag ccgcgattca
     1321 aagtgatcgg ctcggatctt tggtccgatg agcccggatt cgccgcggca accgccgcca
     1381 cgggcatcac cggcgtgtgc gggtccggga tcatcgaggt catcgccgaa atgtacctcg
     1441 ccggcatcat caaccaagat ggtgtcctcg acggccgctt ggctgcgcgc tcgccgcgca
     1501 tcgtcgcgaa cggcagaacc ttcgcctatg tgctgcagca agggaccgtc gacatgaaga
     1561 tcacccagaa cgacgtgcgc gccattcagc tggcaaaggc ggcgctgtat gcgggcattg
     1621 cgctgctcat ggagcggctc gggatcgatc acgtcgatcg catccggctc gccgggg
//
LOCUS       DASWZT010000383        15864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_41900, whole genome shotgun sequence.
ACCESSION   DASWZT010000383 DASWZT010000000
VERSION     DASWZT010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15864
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41900"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VGE92_10755"
     CDS             complement(<1..621)
                     /locus_tag="VGE92_10755"
                     /inference="COORDINATES: protein motif:HMM:NF016952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEY1314350.1"
                     /translation="MHRLAIALGCLLAFAPARAAVPTYQAEVVHIYPHDTSAFTEGLF
                     YLNGYLYESTGLEQHSTIRKVRLETGEVVQKLDIPPQYFGEGIVDWHGRLISLTWKSH
                     VGFVFDLKTLKLQRQFQYPGEGWALTHDDKQLIMSDGTSELRLLDPATFNETGRIKVT
                     VEGKPLRNVNELEWVKGEIYANVWQTNWIVRIDPDSGRVVGAIDLTG"
     gene            complement(621..1223)
                     /locus_tag="VGE92_10760"
     CDS             complement(621..1223)
                     /locus_tag="VGE92_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314351.1"
                     /translation="MRCAIFCLAGAAAASAASPAAGLVEPPPAVPSDCLESGDGYFRA
                     RLGGAIDATVDWPNSGTQCQGESKGASPGVRLSFQRAAHSKPDLLFVFGVTGIREGAP
                     AHAAGANLTVIVQGTSRIYGTLGDTRCTVDSLAQRPLKTQRWYRVEARGFCTQPAHAV
                     RGKGEVLVSTFEFAGLVIYDAPATATPALAPAPHPAAPGN"
     gene            1333..1815
                     /locus_tag="VGE92_10765"
     CDS             1333..1815
                     /locus_tag="VGE92_10765"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HEY1314352.1"
                     /translation="MPRKEGSAAMIRNLYLAACWGCLAACSLVAPKFNRPNISVVNVE
                     LRGGNLFQQNFAVKLNVQNPNDRALPVQGLQVALHVAGEEIATGVSDRAFVVPAFGES
                     EFDMTIKSNLAVALLKLADKMNQHADSVDYDLTGTASVDLPFMRDLPFHQTGSFSLNQ
                     "
     gene            1967..3466
                     /locus_tag="VGE92_10770"
     CDS             1967..3466
                     /locus_tag="VGE92_10770"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEY1314353.1"
                     /translation="MAASPYEQLEQEFRRLHAFRGALSLLRWDAAVMMPRGSADVRGE
                     QLAALETEHHALLTTPKVARLLERAEAGASQLEDWPLANLHEMRRQRDHAIATPPSLI
                     SRLAKATARAEVFWVEAKKADDFKLLAPHLEEVVHLVRDKAALLGQARGLAPYDALVD
                     GFTPGVSTADIDTIFKAVSRKLPGLINEAIEVQAAHAPIPLSGKFTVARQRSLSVDVL
                     KALGFPFDRGRLDESEHPFTEGVPGDIRVTTRFDLNDPFSGLLGAVHETGHAMYDLGL
                     PVKWRDQPVGRDRGLALEESQSLLFEMNLCRSRSFVKYLRPLLEKYLQVSGPEWEEAN
                     LYRHLIRVRRSTIRMDADELTYPVHIMLRYELEKKILEGSLPVADLPEAWNANLEQRL
                     NIRPTSDVDGCLQDIHWAVGHFGYFPSYVLGAVIAGQLNEAMRAARPALDEEIASGQF
                     GGVMDWLRDNVHGVGARLPVQELIKQATGKPLTAAAYLRYLEAKYLEVP"
     gene            3468..4340
                     /gene="ttcA"
                     /locus_tag="VGE92_10775"
     CDS             3468..4340
                     /gene="ttcA"
                     /locus_tag="VGE92_10775"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEY1314354.1"
                     /translation="MTLAVRHSNNFKKLQAQLRGDVGKAIADFDMIAAGDHVMVCLSG
                     GKDSYTLLDILLSLQRSAPVRFELTAVNLDQKQPNFPAEVLPRYLENLGVRYRIIEQN
                     TYAVVKRVIPEGKTMCGLCSRLRRGALYRYAAQNGITKIALGHHRDDIVETLFLNMFF
                     GGRMKAMPPKLLSDDRRHIVIRPLAYVSEASIARYAAHAQFPIIPCTLCGSQPQLQRL
                     AVKNLLREWQQKFPGRIESIFSSICNVAGSQLADPALFDFAGLDGRRVTASCDAPESD
                     AAETTMADLHPLET"
     gene            complement(4264..5451)
                     /locus_tag="VGE92_10780"
     CDS             complement(4264..5451)
                     /locus_tag="VGE92_10780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1314355.1"
                     /translation="MTEVIAAGALRPLSTVTIDTVTSLDGVLALKPCYETLQQKIDNP
                     LPFALHEWHVTWCRHFLNANPRIQDELLFYVLRDPAGACVAIIPFVVSRRRIGPIKVV
                     SVNLIGSDPAVTEIRAPMVAPGYERLAAHAVQQHLTKAINWDWIHWTGISAPFAEALA
                     VNGRMQWQPPQEDFVLDLAPSWEEFRGRLKRNIRESLRHCYNSLKRDNHEFEFCVVKE
                     ASQMRSALDRFFELHAMRADLKTAAIHPNRFVNQASRDFLYDICVRLCAQDAVRIFQL
                     KVGPEVVAARVGFIARDGLYLYYSGYDPAWWRYSVMTTTVAEAIKYAIGLGLKTVNLS
                     PNREVSKTRWGPRQVDYIAAYELGARLRSRLARQAYVRFQGGEGLPSWFLQHLIPVRR
                     NWQ"
     gene            5675..6601
                     /locus_tag="VGE92_10785"
     CDS             5675..6601
                     /locus_tag="VGE92_10785"
                     /inference="COORDINATES: protein motif:HMM:NF027219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HEY1314356.1"
                     /translation="MEVAIFRLYRELELRGLGFRPHCWLAQEWFSPDGIPGIAIPFFL
                     AHRRLMRLERRFMREVEGGNRNWLMRILRHEAGHAIDSAYRLRRRGGWRAVFGPASLP
                     YPDTYRPRPGSRRFVQHLGAWYAQAHPTEDFAETFAVWLKPHSAWRREYLGWPAFAKL
                     EYIDGLADEIGRRKPPVSNRATIEEVTRETRTLREHYEQKLARYRMPRRSSADELLLK
                     VFTAAPRGRARIKAASVLRELRNPLRQRLLRSGAFSEYLVHQVLRLMIERCERLSLYL
                     RGPRRELKSQLLWVTARLAESYEERQLPRVSL"
     gene            6607..7632
                     /locus_tag="VGE92_10790"
     CDS             6607..7632
                     /locus_tag="VGE92_10790"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314357.1"
                     /translation="MRALVLVHATLVPPDSLKGSTEKEIDEWRTEYDVISHLKAEGHE
                     VRPLGLSDSLSELRRAILDWKPDIVFNLLEEFDGIVTYDQHVVAFLELMHQPYTGCNP
                     RGMLLSRDKVLSKQLLSYHRVPTPQFMVFARGRKPRIPKKLRFPLFVKSSTDDASLGI
                     AHASVVSDQKQLTERIAFIHEQTHSDALVEEYIEGRELYVGVLGNDRLRVLPVWEMSF
                     GSLPENLPAIATRKVKWDRGYQQRYGITTAAAQTLPDGTVTRLEQLSKRIFRALHLTG
                     YARMDFRLRGDGELFVLEANANPNISTAEDFAQSALTAGMNYDDLLQRILQLGERYPA
                     AWRGVDP"
     gene            complement(7648..9732)
                     /locus_tag="VGE92_10795"
     CDS             complement(7648..9732)
                     /locus_tag="VGE92_10795"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY1314358.1"
                     /translation="MICHRIVAACAAWLGFSAVCSAALGAAADHSLPFTVQDLVRLER
                     VSDIAASPDGTHVVYTLRSTDLDANKGRTAVWLVDTTKRNAPAVRLTDLSANSGSAEW
                     SADGRFVYFLSNRSGSTQVWRVAAQGGDAPGANALQVTDLPLDVGTFRVSPKGDRLLV
                     SVEVFLDCADFACTKQRLDTAAHAKGTGMLYKQLFVRHWDTWSDGRRSQLFALPLNEV
                     AKGTPLNLTAGIGDVPSKPFAGREDYAFSADGTQVAFSVRAAAGEAWSTNFDIYEVAA
                     AGGTPRNLTADNPAWDGRPAFSPDGSQLAYVAMERAGFEADRFHLVVLDLNSGAKHPL
                     TQNWDRSIADFAWARDGKSLFATTDHLGQRPLWAIDAKTGRTSAITGAGEVEGFSVGP
                     RKVFYAQSSLGGPADVYAVGFSGGAAKQLTHVNQAALAQRKFGEFEQFSFAGWNTENV
                     FGYVVKPPDFKRDQKYPVAFVVHGGPQGSLANSWNWRWNAQTFAGAGYAVVMIDFHGS
                     TGYGQAFTDSISGDWGGKPLEDLKLGLEAALKQFPWLDGEHACALGASYGGFMMNWLA
                     GQWPDQFKCIVSHDGIFDNRTMYYSTEELWFPEWEFGGPEYRKPAGYAKFNPVDYVKK
                     WKTPTLVIHGQLDYRVPDSQGLAVFTALQRQGVPSELLYFPSENHWVLKPGDSIQWYD
                     AVLDWLNRWTRH"
     gene            9811..10491
                     /locus_tag="VGE92_10800"
     CDS             9811..10491
                     /locus_tag="VGE92_10800"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY1314359.1"
                     /translation="MVENTAPRVLSFDLDDTLWPVAPVIAAAESELLTWLRARYPRTV
                     SGHDGESLRTLRAQVAERFPQHGHDLTFLRHRALKDLFGAAGHAESLADEALEVFFQA
                     RNRVQFYDDALPALKRLRARYRLFAISNGNADLQRCGIADLFSGHITASAAGAAKPDA
                     RIFAKLEAIAGVAAAEVLHIGDDPLADVIGARQAGMQAVWLNRNAREWPALLARPPRT
                     ISTLAEIL"
     gene            10655..11281
                     /locus_tag="VGE92_10805"
     CDS             10655..11281
                     /locus_tag="VGE92_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314360.1"
                     /translation="MQAVSGSLVAVSSLREFFRDAFHAASERQHLDIDEQAEQYVVNL
                     LTMFSRAEALYERTPDGLRIRPLAHMLADALEAPTTVARQRGLQRLGDVSLFIAGFFA
                     RSFARKLIDIDYHIAMGGNAYSSLADTMHRSVGGRCVAAIYAQLASKFQPLVDALNDV
                     SEMSYQHTDADILRLYEIWMKTGSSRAHGLLKKLGVQPVAAGGARREH"
     gene            11287..11940
                     /locus_tag="VGE92_10810"
     CDS             11287..11940
                     /locus_tag="VGE92_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314361.1"
                     /translation="MVLQGLQSLLERLYDIDIDYDVYDFLITDRGVLPGLAPAAVTHD
                     TEEELLLAETAEGAGMALYLDPELLRRLEGADPVGALTEGNLADYCTALEGVSHFVYS
                     AWRLDRDLPVSLLELETQAEVDKYAVTVFLLADQQGGGYPAHVHPRLFDRVSFDVRLE
                     PDQYARYLTAHRSAAQYCRRLERRFVTRGEARIEALVRELRKFYRLGSAAKLRHALA"
     gene            complement(11960..12373)
                     /locus_tag="VGE92_10815"
     CDS             complement(11960..12373)
                     /locus_tag="VGE92_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314362.1"
                     /translation="MNGKNSKPYPDADEFGADDPTEPSFDRDDVGTGRVKFDDRGNAV
                     WEWAVSTGAFGNELSPERLQKLEHPALSIADEPQPVAETVRANPLGTKKGYDPYDSGK
                     LGKAAAAPRKKKDLRRLGEFLKLKKQAASNKQSED"
     gene            complement(12437..12970)
                     /locus_tag="VGE92_10820"
     CDS             complement(12437..12970)
                     /locus_tag="VGE92_10820"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY1314363.1"
                     /translation="MQKPIPISSTVVHRGRIIEVSTERLHYGPGREFDLDFVKHPGAA
                     AVVAIDDSDRVCLVRQYRHGIGDFLWEIPAGKLDAGEAPQICAVRELAEETGVNAKRW
                     ASLGQFYPAPGIFTEIIHLFLASELDVGAAAPDADEELEIQWLPFTAAVEMVLRGELN
                     DGKTAMALWRAQHRRQL"
     gene            13090..15177
                     /locus_tag="VGE92_10825"
     CDS             13090..15177
                     /locus_tag="VGE92_10825"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEY1314364.1"
                     /translation="MMKAKPTAFDSLNPSQRRAATFGTAIPDKGVDAGPMLILAGAGT
                     GKTNTLAHRAAHLVLNGVDPARILMLTFTRRAAQEMIRRTQGIVAQVLADRGKAGDRS
                     VTSRLLWSGTFHSVGNRILRLYAKHLGLDPNFTVLDRADAADLMDVVRHELGFSAKDK
                     RFPRKDACLAIYSYRVNTRLSLKQTLEEQFPWCREWEADLTRLYREYVGRKQKDHALD
                     FDDLLLYWHVMMQNPALAQSLSKNFDHLLVDEYQDTSTLQGEVVHALKPDGKGVTVVG
                     DDAQAIYSFRAAAVENILGFAERFKPKAEIVVLSQNYRSTQQVLDSANALMADGARQH
                     RKTLLGTRQSTQKTLYVAMDDAVAQAEYITNKILATREIGGSLKRHAILFRSSHHSDV
                     LEVELTKRDIPFVKYGGLKFLEAAHVKDMMSVLRWADNPHNSVAGFRVLKLLPGIGPG
                     HAKQALDYFEAQNFAIKSLANFDAPQPVKMDWKRFCILLEKLADPATPWAGQVGLVRE
                     WYKPQLERIYDSAWTRMGDLEQLEQLSLQHTTRERFLTELTLDPPVVTGDQSGAASKD
                     EDYVILSTIHSAKGQEWDIVYVLNVSDGNFPSEFSTGKPEMIEEERRLLYVAMTRARN
                     ELHLCAPLKYAVTQQAKDGDKHVFGAKSRFMTDKVLESCERISLRNMRGVEGLKEGET
                     ATVDVVAQLKEMW"
     gene            complement(15254..15556)
                     /locus_tag="VGE92_10830"
     CDS             complement(15254..15556)
                     /locus_tag="VGE92_10830"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY1314365.1"
                     /translation="MTTIYVGNLPFTATEDEVRALFERHGKVESVKLINDRETGRPRG
                     FAFVDMGASEAQTAIQQTNGFQMGGRPLRVNEAQERAPRSKPAGGGGGGGSGPRRW"
     gene            complement(15675..>15864)
                     /locus_tag="VGE92_10835"
     CDS             complement(15675..>15864)
                     /locus_tag="VGE92_10835"
                     /inference="COORDINATES: protein motif:HMM:NF037681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314366.1"
                     /translation="VKGDTANKVLEYMEYDTDELYPAIARDCERAVQEGRMSLTEAQA
                     VKRFYELELNGYAYLEVE"
BASE COUNT         2970 a         4886 c         4944 g         3064 t
ORIGIN      
        1 ccccgtgagg tcgatcgcgc cgaccacgcg gccgctgtca gggtcgatgc gcacgatcca
       61 gttggtctgc catacgttgg cgtagatctc ccccttcacc cattccaact cgttcacgtt
      121 gcgcaagggt ttgccctcga cggtcacctt gattcggccg gtctcgttga acgtcgcagg
      181 atcgagcaag cgcagctccg aagtgccgtc gctcatgatg agttgcttgt cgtcgtgagt
      241 gagcgcccat ccctcgccgg ggtattgaaa ctgtcgctgc agcttcaagg ttttcaggtc
      301 gaacacgaag ccgacgtgcg acttccaagt gagactgatg aggcgcccgt gccagtcgac
      361 gatgccttcg ccaaaatact gcggcggaat gtcgagcttc tgcacgacct cgccggtctc
      421 gagccgcact ttgcggatgg tggaatgttg ctcgagcccc gtgctttcat acagatagcc
      481 attcaagtag aacagccctt cggtgaatgc cgaggtatcg tgcggataga tgtgcacaac
      541 ctccgcctgg taggtcggca cggcagctcg cgctggcgcg aaggccaata ggcaaccgag
      601 cgcaatcgcc aaccggtgca tcagttgcca ggtgcggccg ggtgcggggc cggggcgagg
      661 gccggagtcg cggtggcggg tgcgtcgtag atgacgagtc cagcgaattc aaaggtgctt
      721 accaacacct cgcccttgcc gcgcaccgcg tgtgccggtt gcgtgcaaaa accgcgtgcc
      781 tcgactcgat accagcgctg tgttttgagc ggccgctgcg ccagcgagtc gaccgtgcag
      841 cgcgtgtcgc cgagggtgcc gtaaatccgg ctggtgcctt gaacgatcac cgtcagattt
      901 gcccccgcgg catgtgccgg tgccccttca cggatgccgg tgacgccgaa cacgaacagc
      961 aggtccggtt tgctgtgggc ggcgcgctga aagctcagac gcacgccggg cgatgcgccc
     1021 ttcgattcgc cctgacactg cgtgccgctg ttgggccaat cgaccgttgc gtcaatcgcg
     1081 ccgccgaggc gcgctcgaaa atagccatcg cccgattcga gacagtcgga gggcacggcg
     1141 ggcggcggct cgaccaggcc ggccgccggc gacgccgcgg aggcggccgc agcccccgcc
     1201 aggcaaaaga tcgcgcagcg caccgctcgc cggcggcgcg gagcaaatga gcggtgagat
     1261 gtgctggtcg atcgcatcgg atttaagagg cggcattgtg ccacgagcca ggcacacgat
     1321 cgctcggata gaatgccgcg caaggagggc agcgccgcga tgattcgaaa cctgtatctg
     1381 gccgcatgtt gggggtgcct ggctgcctgt tctctggtgg cgccgaaatt caatcggccc
     1441 aatatctcgg tcgtgaacgt cgaactacgc ggcggtaacc tgttccagca aaactttgcg
     1501 gtcaagctga acgtgcaaaa cccgaacgac cgggcattgc cggtgcaagg gctgcaggtc
     1561 gcgcttcacg tggcaggcga ggagatcgca accggcgtca gcgaccgcgc gttcgtggtt
     1621 cccgcattcg gcgaatccga gttcgacatg accatcaagt ccaatttggc ggtcgcgttg
     1681 cttaagttgg ccgataaaat gaatcagcac gcggactcgg tcgattacga tctgaccggc
     1741 accgcgagcg tcgatttgcc cttcatgcgc gatctgccgt ttcatcaaac ggggtctttc
     1801 tcactcaacc agtagcggct cggcggcgcc cgcttgcgag ggggttcacg cccccgcgtt
     1861 cgcttattta tgtgttaact gattgaaata attgcggttt aggatcttgt aaagccgaat
     1921 cggggtcgcc atctggtaga cttctgagcc tacctcgaag gttcccatgg ctgcctcccc
     1981 gtacgaacaa ttagaacaag agtttcgccg tctccatgcc ttccgcggcg cgttatcgct
     2041 gctgcgctgg gatgcggccg tgatgatgcc gcgcggcagc gccgacgtga ggggcgagca
     2101 actggcggcg ctcgagaccg aacatcacgc tctgctcacc acgcccaaag tggcgcgcct
     2161 actcgagcgc gccgaagccg gtgcctcgca actcgaggat tggccgctcg ccaacctgca
     2221 tgaaatgcgg cgccagcgcg accatgcgat cgcgacgccg ccttcgctga tctcgcgcct
     2281 cgccaaggcc acggcgcgcg ccgaggtgtt ctgggtcgaa gccaagaagg ccgacgactt
     2341 caaattgctc gcaccccatc tggaagaggt ggtgcatctg gtacgcgaca aggcggcgct
     2401 gctcggtcag gcgcgcggcc tggcacccta tgatgcgctc gtggatgggt tcacgccggg
     2461 tgtgtcgacg gcggatatcg acaccatttt caaggctgtg tcgcgaaaat tacccggcct
     2521 catcaacgag gccatcgagg tgcaagccgc gcatgcgccg atacccttgt ccgggaaatt
     2581 cacggtggcg cgccagcgct cgctatccgt cgatgtgctg aaagccctgg gtttcccatt
     2641 cgaccgcggg cgcctcgacg aaagcgagca cccgttcacc gagggggtgc cgggcgatat
     2701 ccgcgtgact acgcgcttcg atttgaacga tccgttttcc gggttgttgg gcgcggtgca
     2761 cgagaccggt cacgccatgt acgacttggg cttgccggtc aaatggcgcg atcagccggt
     2821 cggtcgcgac cgcggactgg cgctcgagga aagccagtcg ctgctgttcg agatgaacct
     2881 gtgccgcagc cgctcgttcg tcaagtactt gcggccgttg ctcgagaaat atttgcaggt
     2941 gagcggcccg gagtgggaag aggcgaattt gtaccgtcac ctgatccgcg tacgccgcag
     3001 caccattcgc atggacgccg acgagctcac ctacccagtc cacatcatgc tgcgctacga
     3061 gctcgagaaa aagattctcg agggatcgct gccggtcgcc gacttgccgg aggcgtggaa
     3121 cgccaacctc gagcagcggc tgaacattcg tccgacgagc gatgttgatg gctgcctgca
     3181 ggacattcat tgggccgtcg gtcacttcgg ttactttcct tcctacgtgc tcggcgccgt
     3241 catcgcaggc cagttgaacg aagcgatgcg cgccgcgcgg ccggcgctcg atgaggaaat
     3301 cgcgagcggt caattcggcg gtgtgatgga ctggctgcgc gacaacgtac acggcgtcgg
     3361 cgcgcgcttg ccggtgcagg aactcatcaa gcaagcgacc ggcaagccgc tgacggcggc
     3421 cgcgtacctg cgctacctcg aagcgaaata cctcgaggtt ccttgaggtg accctcgcgg
     3481 tccgccattc caacaatttc aagaagttgc aggctcagct gcgcggcgac gtgggcaaag
     3541 caatcgccga tttcgacatg atcgccgctg gcgatcatgt gatggtatgc ctgtccggcg
     3601 gcaaggattc gtacacgctg ctcgatattt tgttgagcct gcagaggagc gctcccgtgc
     3661 gctttgaact cactgcggtc aatcttgatc agaagcagcc caattttccg gccgaggtct
     3721 tgccgcgcta tctcgagaat ctgggagttc gctaccggat catcgaacag aacacctacg
     3781 cggtggtcaa gcgcgtcatc cccgagggca aaaccatgtg cggcctctgc tcgcgactgc
     3841 gccgcggcgc cctgtaccgc tatgccgcgc agaacggtat tacgaagatc gcgctcggcc
     3901 atcatcgcga tgacatcgtc gagacgcttt tcttgaacat gttcttcggc gggcgcatga
     3961 aggcgatgcc gccgaaactg ctgagcgatg atcgccgcca cattgtgata cggccgctcg
     4021 cctacgtcag cgaggcgtcg attgcgcgct acgccgcgca cgcccaattt ccgatcattc
     4081 cctgcacgct gtgcggctcg cagccgcaac tgcagcggct cgccgtgaag aacctgctgc
     4141 gcgaatggca gcagaaattc cccggccgga tcgagtcgat attttccagc atttgtaacg
     4201 tcgccggctc gcagctcgcg gacccggcgc tgttcgactt cgcgggcctc gatggccgca
     4261 gggttactgc cagttgcgac gcaccggaat cagatgctgc agaaaccacg atggcagacc
     4321 ttcaccccct tgaaacctga cgtaggcctg gcgcgccaag cgcgaacgca ggcgcgcgcc
     4381 gagttcgtaa gcggcaatgt agtcgacttg ccgcggcccc caacgggttt ttgagacctc
     4441 tctgttgggt gagagattga cggtcttgag acccaggccg atggcgtatt tgatggcttc
     4501 cgcgaccgtg gtcgtcatga cgctgtagcg ccaccatgcg gggtcgtatc cggaatagta
     4561 caggtagagg ccgtcgcggg cgatgaagcc gacccgtgcc gccaccactt ccggaccgac
     4621 cttcagttgg aatatgcgca ctgcgtcctg ggcgcacaag cgcacgcaaa tgtcgtacag
     4681 gaaatcccgg gaagcctgat ttacgaagcg attcggatgg atcgccgccg tcttcaaatc
     4741 ggcccgcatg gcatgcaatt cgaaaaatcg gtcgagcgcg ctgcgcatct gcgacgcttc
     4801 tttgaccacg cagaattcga attcatggtt gtcgcgcttc agcgagttat agcaatgacg
     4861 caacgactcg cggatgttgc gcttgaggcg cccgcggaac tcctcccagc tcggcgccaa
     4921 atcgagaacg aaatcttctt gcggcggctg ccattgcatt ctgccattga cggccaacgc
     4981 ttcggcgaac ggcgcgctga tcccggtcca gtgaatccaa tcccaattaa tcgccttggt
     5041 cagatgctgt tgcaccgcgt gcgccgccaa gcgctcgtag cccggcgcga ccatgggcgc
     5101 gcgaatctcg gtgacggcgg ggtcgctgcc gatcaaattg accgagacaa ccttgatcgg
     5161 gccgatgcgg cgccggctga cgacgaaggg aatgatggcg acgcaggcgc cggccgggtc
     5221 gcgcaacacg tagaacaaca gctcatcctg aatccgcgga ttggcattca ggaaatgccg
     5281 gcaccaggtg acatgccact cgtgcagggc aaacggcagc gggttgtcga tcttttgctg
     5341 cagcgtctcg tagcagggct tgagcgccag aacgccgtcc aacgaggtca cggtatcaat
     5401 cgtaaccgtg ctcaaaggac gcaatgcgcc ggcggcaata acttcagtca aattagctat
     5461 agcgtttaat gaacgctctc cgtccgggtc gcattcggat tgtaaaatct acgagttacc
     5521 agtattagta tcacacacta tgatgcctat ccgtaagcag gctgccacca cttcgcggtc
     5581 gcagaagcgc caccgcgcgt gggagaaact gcccgacgag caattgctca attttcgatt
     5641 ctgtgacctg cgtctcacta ttgaaggcac tgagttggaa gttgccatat ttcgcctgta
     5701 tcgcgaactc gagctgcgtg gcctaggatt tcggccgcac tgctggctcg cgcaagagtg
     5761 gttctcgccc gatggcatac ccggcatcgc cattccgttc tttctcgcgc accgccgctt
     5821 gatgcgcttg gagcggcgct tcatgcgcga agtcgagggc ggcaaccgca actggctgat
     5881 gcgcatactg cggcacgaag caggccatgc aatcgattcc gcctaccgct tgcggcgccg
     5941 cggcggctgg cgtgcggtat ttgggcccgc ctcgctgccg tatcccgaca cttaccgtcc
     6001 gcggcccggg agccgccgtt tcgtgcagca tttgggggcg tggtacgcgc aggcgcaccc
     6061 gaccgaggac tttgccgaga ccttcgcggt gtggctcaaa ccgcattccg cgtggcgccg
     6121 cgaatatctc gggtggcctg cttttgcaaa actcgagtac atcgatgggc tggcggatga
     6181 gattgggcgg cgcaagccgc cggtgtcgaa ccgcgccacg atcgaggagg tcacgcgtga
     6241 aacgcgcacc ctgcgcgaac actacgagca aaagctcgcg cgctatcgca tgccgcgccg
     6301 ctccagcgca gacgaattgc tcctcaaggt gttcacggcg gcgccgcgcg gccgcgcgcg
     6361 catcaaagcg gcgagcgtgc tgcgcgaact gcgcaacccc ttgcgccagc ggctgctgcg
     6421 ctctggcgcc ttcagcgaat acctggtcca ccaggtgctc aggctgatga tcgagcgttg
     6481 cgaacggttg agcttgtacc tgcgcggccc gcgccgcgag ctcaaatctc agttgttgtg
     6541 ggtgaccgcg cgcctcgccg agtcctacga agagcggcaa ttgccgcggg tgtcgctgtg
     6601 aaaaaaatgc gcgccttggt gttggtgcac gcgacgctcg tgcccccgga ttctctgaag
     6661 ggctcgaccg agaaggaaat cgacgaatgg cgcaccgaat acgacgttat ctcgcatttg
     6721 aaggcagagg gtcacgaagt gcggcccttg gggcttagcg acagcctgtc cgagttgcgg
     6781 cgtgcgattc tcgattggaa gcccgacatc gtctttaatt tgctggaaga gttcgacggc
     6841 atcgtcacgt acgaccagca cgtggtcgcg tttctcgaac tcatgcatca accctacacc
     6901 ggctgcaatc cgcgcggcat gctgctgtcg cgcgacaaag tgctgtcgaa gcaattgctg
     6961 tcctaccatc gggtgccgac tccgcagttc atggtattcg cgcgcggtag aaagccgcgg
     7021 attccgaaga agctgcgctt ccccttgttc gtcaaatcat cgaccgacga tgcctcgctc
     7081 gggatcgcgc acgcctccgt ggtctcggat cagaagcaat tgaccgaaag aattgccttc
     7141 atccacgaac agacccactc cgacgcgctg gtcgaagagt acatcgaggg ccgcgaactc
     7201 tatgtgggcg tgctcggcaa cgaccggctg cgggtgctgc ccgtgtggga gatgagtttc
     7261 ggatcgctgc cggagaacct gccggcgatc gcgacgcgca aagtgaaatg ggaccgcggc
     7321 tatcagcagc gctacggcat caccaccgct gcggcgcaaa cactgcccga cggcaccgtg
     7381 acgcggctcg aacaattgtc gaagcgcatt ttccgcgcgt tgcatctgac gggctacgcg
     7441 cgcatggatt ttcgcctgcg gggcgacggc gagctgttcg tgctggaggc gaacgccaac
     7501 ccgaacatct ccaccgccga ggactttgcg caatcggcgc tgacggccgg catgaactac
     7561 gacgatctgc tgcagcggat cctgcagctc ggcgagaggt atccggcggc ctggcgcggt
     7621 gtcgacccgt gaggccggga cgttccctca gtgacgcgtc cagcgattca accagtcgag
     7681 cacggcgtcg taccattgga tgctgtcgcc cggcttcaag acccagtggt tttcgctcgg
     7741 gaagtacaac aattcgctcg gtacgccttg acgttgcagc gcggtgaaca ccgccaaccc
     7801 ctgcgagtcg gggacgcgat agtccaactg gccgtgaatc accagtgtcg gtgttttcca
     7861 tttcttcacg tagtcgacgg ggttgaattt ggcatatccg gccggcttgc gatattcagg
     7921 tccgccgaat tcccactcgg gaaaccacaa ttcctcggtg gagtagtaca tggtgcgatt
     7981 gtcgaaaatg ccgtcgtggc tgacgatgca cttgaactgg tcgggccact ggccggcgag
     8041 ccaattcatc atgaaaccgc cgtaagaggc tcccaacgcg cacgcatgct cgccgtccag
     8101 ccaagggaac tgcttgagcg ccgcctccag gcccagcttt agatcctcga gcggtttacc
     8161 gccccagtca ccgctgatcg aatcggtgaa tgcctgcccg taacccgtcg aaccgtggaa
     8221 atcgatcatg acgacagcgt agccggcgcc ggcaaaagtc tgcgcattcc agcgccaatt
     8281 ccacgagttc gcgagactgc cttgcgggcc gccgtgcacc acgaaggcca ccggatattt
     8341 ctgatcgcgc ttaaaatccg gcggtttgac cacgtagccg aaaacattct cggtgttcca
     8401 accggcaaag ctgaactgtt cgaactcgcc gaatttgcgc tgcgcgaggg ccgcttgatt
     8461 cacgtgcgtg agctgtttgg cggcgccgcc tgagaagccg accgcataca cgtcggcggg
     8521 cccgccgagg ctgctttgcg cgtagaagac cttgcgcggc ccgacgctga agccttcgac
     8581 ctcgccggcg ccggtgattg ccgacgttct gccggttttg gcatcgatgg cccatagcgg
     8641 ccgctgaccc aggtggtcgg tggttgcgaa caggctcttg ccgtcgcgcg cccaggcgaa
     8701 atcggcgatc gatcggtccc agttctgggt cagcgggtgt tttgcgccgg aattcaaatc
     8761 gagcactacc agatgaaagc gatccgcctc gaaacccgcc cgctccatcg ccacgtaggc
     8821 gagctggcta ccgtcgggcg agaaggccgg gcgcccgtcc cacgcgggat tatcggcggt
     8881 caaattgcgc ggggttccgc ccgcggcggc cacttcgtag atgtcgaagt tggtggacca
     8941 tgcttcgccc gcggcggcgc gcaccgaaaa tgccacctgt gtcccgtccg cgctgaacgc
     9001 atagtcctcg cggccggcga agggcttgct cggcacatcg ccgattccgg ccgtaagatt
     9061 cagcggcgtg cccttcgcca cctcgttgag cggcagggcg aacagctgcg agcggcggcc
     9121 atcgctccac gtgtcccagt ggcggacgaa cagttgcttg tagagcatgc ccgtaccttt
     9181 cgcgtgcgcg gccgtgtcca accgctgctt ggtacaggca aaatcggcgc aatccaggaa
     9241 cacctcgacg ctgaccaaga gccggtcgcc tttcggagag acgcgaaagg tgccgacatc
     9301 gagcggtaaa tcggtcactt gcagcgcatt cgcaccgggt gcatcgccgc cctgcgccgc
     9361 cacccgccat acttgggtgg atccgctgcg gttggaaaga aaataaacga aacgcccatc
     9421 ggcgctccac tcggcggaac cggagttcgc cgacagatcg gtgagacgga ccgccggggc
     9481 gttgcgtttg gtcgtgtcga ccagccaaac ggcggtgcgc cccttgttgg cgtcgagatc
     9541 ggtgctgcgc agcgtgtaga cgacgtgcgt gccgtccggc gatgcggcga tgtcggaaac
     9601 gcgctccagc cgcaccaggt cttgcaccgt gaagggtagc gagtggtctg cggccgcgcc
     9661 gagtgcggca ctgcatacgg cactgaagcc gagccaagca gcacaggctg cgacgatacg
     9721 atggcaaatc attgcggttc cctaaattgt tttgattttc ctaaagcccg gccagcgcca
     9781 agataaaggt tcgcaggcta acatggctgc atggttgaaa acacggcgcc gcgggttttg
     9841 agctttgacc tggacgatac cctgtggccg gtggctccgg tgatcgcagc cgccgagagc
     9901 gaattattga cctggctgcg cgcccgctat cctagaaccg tgagcggcca cgacggcgaa
     9961 tcgctgcgta ctctgcgcgc acaggtcgct gagagatttc cgcagcacgg ccatgatctc
    10021 acctttctgc gccatcgagc gctcaaggat ctgttcggtg ccgccggtca cgcggagtcg
    10081 cttgcggatg aggcgctcga ggtgtttttt caggcgcgca atcgggttca attctacgac
    10141 gatgcgttgc ccgcgcttaa gcggctgcgt gcgcgctatc gcttgttcgc catcagcaac
    10201 ggcaatgcgg atttgcagcg ctgcgggatt gcggacttgt tcagcggcca catcacggca
    10261 agcgccgcgg gcgcagcgaa acccgacgcg cgtatttttg cgaagctcga ggcgatcgcg
    10321 ggcgtggcgg cggccgaggt gctgcacatc ggcgatgatc cattggccga tgtgatcggt
    10381 gcgcgccaag caggcatgca agccgtatgg ctgaaccgca acgcgcgcga gtggccggcg
    10441 ctgttggcgc ggccgccgcg caccatttcg acgctcgcgg aaattctcta gcaccaatga
    10501 gatgcagcgc acgcttagca gcctatcggc cggactgctg attaagaaaa ccctgagaat
    10561 atgggcattt tctgacactg gttcgcgctt ttgcgcttca cttgtgcgta gttttgtcgc
    10621 tacgatggaa gcagctgaaa aaaggatgcc gagcatgcaa gcagtatctg gatctcttgt
    10681 agcggtcagt tctttacgcg aattttttcg tgacgcgttt cacgcggcga gcgaacgcca
    10741 gcatctcgac atcgatgaac aggccgagca atacgtggtc aatctgctca ccatgttctc
    10801 gcgcgccgaa gcgttgtacg agcgcacgcc ggatggtctg cgcatccggc ctctggcgca
    10861 catgctcgcc gatgcgctcg aggcgccgac gacggtggcg cggcagcgcg gattgcagcg
    10921 cttgggcgac gtttcgctgt tcattgccgg cttcttcgcg cgcagcttcg cgcgcaaact
    10981 gatcgatatc gactatcaca tcgccatggg cgggaacgct tacagctcgc tcgcggatac
    11041 catgcaccgc tcggtgggag ggcgatgcgt cgcggcgatc tatgcgcagc tcgccagcaa
    11101 atttcagccc ctcgtggacg ccctgaacga tgtcagcgaa atgtcgtatc aacacaccga
    11161 cgcggatatt ctgcggctgt atgagatctg gatgaaaacc ggcagttcgc gggctcacgg
    11221 cctgttgaag aagcttggcg tgcagccggt cgctgcgggc ggcgcacggc gcgagcactg
    11281 agcgcaatgg ttctgcaagg cctgcagtcg ctgctcgaac gcctctacga tatcgacatc
    11341 gactatgacg tctacgattt tctaatcacg gatcgcggcg tgctgccggg gctcgcgccg
    11401 gcggccgtca cgcacgacac cgaagaggaa ttgctgctgg cggaaacagc ggagggcgcc
    11461 gggatggcgc tgtatctgga tcccgagcta ttacgtcgtc tggagggcgc ggatccggtc
    11521 ggcgccttga cggaaggcaa tctggcggac tactgcactg ccctcgaggg cgtcagccac
    11581 ttcgtgtatt cggcctggcg gctggatcgc gatttgcccg tgtcgctgct cgaactcgaa
    11641 acccaggccg aagtcgataa atatgcggtg acggtatttc tgttggccga ccagcagggg
    11701 ggcggctatc ccgcgcacgt gcatccgcgg ctgttcgatc gtgtgagctt cgacgtgcgc
    11761 ctggagccgg atcaatacgc ccgctacctt accgcgcacc gctctgccgc gcagtattgc
    11821 cgcagactcg agcgccgctt cgtgacccgc ggcgaagcgc gaatcgaggc gctggtgcgt
    11881 gaattgcgca aattctatcg gctgggaagc gccgcaaagt tgcgtcacgc gctggcgtga
    11941 tcggcgcgtc gattgcagcc tagtcctcgc tctgcttatt actggcggct tgctttttca
    12001 acttgaggaa ctcgcccaag cggcgcaaat cctttttctt gcgcggcgca gccgcggctt
    12061 tgccgagctt gccgctgtca tacggatcgt agcctttctt ggtgccgagc ggattggcac
    12121 gcacggtctc ggcgaccggt tgcggctcgt cggcgatcga tagcgccggg tgctcgagtt
    12181 tctgcagccg ctccggcgac aactcattgc cgaatgcgcc ggtgctgacg gcccattccc
    12241 acacggcgtt gccccggtcg tcgaatttga cccggcccgt gcccacgtca tcccggtcga
    12301 agctgggctc cgtgggatcg tccgctccga actcgtcggc atcggggtaa ggttttgaat
    12361 tctttccgtt cacgctctct ttgtaatgac gcgccaggtt aagttcaacc tgaacgatcc
    12421 gaaacctggc gaaccgtcac agttgtcggc gatgttgagc gcgccacagc gccatggcgg
    12481 tcttgccgtc attcagttcg ccgcgcagca ccatttcaac ggctgccgtg aagggcagcc
    12541 attgaatctc gagttcttcg tccgcgtcgg gagccgcggc tccaacgtcc aactcgctcg
    12601 cgaggaacag atgaataatc tcggtgaata tgcccggggc ggggtagaac tgtccgagcg
    12661 aggcccagcg tttcgcgttc acgccggtct cttcggccag ctcgcgcacc gcacaaattt
    12721 gcggcgcctc gccggcgtcg agtttgccgg cggggatctc ccacagaaaa tcgccgatgc
    12781 cgtgccggta ctggcgtacc aaacagaccc ggtcggaatc gtcaatggcg actaccgccg
    12841 cggctccggg atgcttgacg aagtcgaggt cgaattctcg gccgggaccg tagtgcaatc
    12901 gctcggtcga gacctcgatg atgcggccgc ggtgcaccac ggtgctcgag atgggaattg
    12961 gtttctgcat gggcgaagtc tacatgtgac ctgcctcgtg gcgcgggacc acggagatgc
    13021 gcgatccttc gggccacttt aaacttattg catcgcaatc tggatattcg tacagtaccg
    13081 gcccaggtca tgatgaaagc caagcccact gctttcgatt cgttgaaccc gtcgcagcgg
    13141 cgggccgcaa ccttcggcac ggcgattccc gacaaggggg tcgatgcggg gccgatgctc
    13201 atcctggccg gcgccggcac cggcaagacc aataccctgg cgcaccgtgc ggcccatctg
    13261 gtgctgaacg gggtcgatcc ggcacggatc ctgatgctga ccttcacgcg ccgcgcggcg
    13321 caggagatga ttcggcgcac ccagggcatc gtcgcccaag tgttggcgga ccgaggcaaa
    13381 gccggcgacc gcagcgtgac gtcgcggctg ctgtggtcgg ggacctttca ctcggtgggc
    13441 aaccgcattc tgcggctgta tgccaaacat ctcggtctcg atccgaactt cacggtgctg
    13501 gaccgcgccg acgccgccga cctcatggac gtggtgcggc acgagcttgg gttctctgcc
    13561 aaagacaagc gtttcccgcg caaggacgcc tgtctcgcca tctactccta ccgggtcaat
    13621 acgcgtctgt ctctgaaaca aacgctggaa gagcaattcc cgtggtgccg tgaatgggaa
    13681 gcggatctga cgcgcctata tcgcgaatat gtcggacgca agcaaaagga tcacgccttg
    13741 gatttcgacg acctgctgct ttactggcac gtcatgatgc agaacccggc cttggcgcag
    13801 agcttgtcga agaacttcga tcacctgctg gtcgatgaat atcaggacac cagcacgctg
    13861 cagggtgaag tcgtgcacgc gctcaagccc gacggcaagg gggtcaccgt ggtcggcgat
    13921 gatgcccagg ccatctactc gtttcgcgcc gcggcggtcg agaacatcct gggttttgcc
    13981 gagcgcttca agcccaaggc cgagatcgtg gtcctgtcgc agaattatcg ctcgacgcaa
    14041 caggtgttgg attccgccaa tgcgctgatg gcggacggcg cgcgccagca ccgcaagaca
    14101 ttgctgggaa cgcgtcaatc cacccagaaa acgctgtacg tggccatgga cgatgccgtc
    14161 gcccaggccg aatacatcac caacaagatt ttggcgactc gcgagatcgg cggttcgttg
    14221 aaacgccacg ccatcctgtt tcggagctcg caccacagcg acgtgctcga ggtcgaactg
    14281 accaagcgcg atattccgtt cgtcaaatac ggcggtctta aattcttgga agccgcccac
    14341 gtcaaagaca tgatgtcggt gctgcgctgg gccgacaatc cgcacaattc ggtggcgggc
    14401 ttcagagtgc tgaagctgct gccgggcatt gggccgggcc atgcgaagca agcgctcgac
    14461 tatttcgagg cgcagaactt cgccatcaag agtctcgcca acttcgatgc gccgcagccg
    14521 gtaaaaatgg attggaagcg attttgcata ttgcttgaaa agctcgccga tcccgcgact
    14581 ccctgggccg gccaagtggg gctggtgcgc gagtggtaca agccgcagct cgagcgcatc
    14641 tacgactccg cctggactcg catgggcgat ctggaacagc ttgagcagct gtcgctgcag
    14701 cacaccaccc gcgagcgatt cctgaccgaa ttgaccctcg atccgccggt ggtgaccggc
    14761 gatcaatcgg gcgccgcctc caaggacgag gactacgtca tcctgtcgac gattcattcg
    14821 gccaagggcc aggaatggga catcgtctat gtgttgaatg tttccgacgg caactttccg
    14881 tcggagttct ccacgggcaa gccggaaatg atcgaggaag agcggcggct gctatacgtg
    14941 gcgatgaccc gcgcgcgaaa cgaactgcac ctgtgcgcgc cgctcaagta cgcggtcacg
    15001 cagcaggcca aggacggcga caagcatgtg ttcggcgcga agagccgctt catgaccgac
    15061 aaggtgctcg agagctgcga gcgaatctct ctgcgcaaca tgcgcggcgt ggagggtttg
    15121 aaggaagggg aaacggcaac cgtcgacgtg gtggcgcaac tcaaagaaat gtggtgagta
    15181 cagacggctg tacccgatcg caaatcaacg cggccttacc ccaagccgcg aaacatcacc
    15241 ggccggcgcg agatcaccag cgccgcgggc cggaaccgcc gccgccaccg ccgccggcag
    15301 gcttggagcg cggagcccgt tcctgggcct cattgacgcg cagcggtcgc ccacccattt
    15361 ggaagccgtt cgtttgttga atcgcggttt gggcttcact cgctcccatg tccacgaacg
    15421 caaagccgcg ggggcgcccg gtttcgcgat cgttgatgag tttgaccgat tcgactttgc
    15481 cgtggcgttc gaacagcgcc cgaacttcgt cttccgtcgc agtgaatggc aggttgccaa
    15541 cgtagatcgt cgtcattcaa ctaatctctc taacagtgtt tcaacactca gacgccgccg
    15601 cgcgaatgcc aacacgccgg agcctgcacc cgagtcgatg ctacgccagg ggattattgt
    15661 tggcaaacgg gcctttattc gacctcgagg tatgcgtaac cgttaagttc gagctcgtaa
    15721 aagcgcttca cggcctgtgc ttcggtgagc gacatccggc cctcttggac cgctcgttca
    15781 cagtcgcggg cgatcgccgg atagagctcg tctgtgtcat attccatgta ctcgagcacc
    15841 ttgttggccg tgtcgccttt gacg
//
LOCUS       DASWZT010000384         8222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_22496, whole genome shotgun sequence.
ACCESSION   DASWZT010000384 DASWZT010000000
VERSION     DASWZT010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8222
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22496"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1916)
                     /locus_tag="VGE92_10840"
     CDS             complement(<1..1916)
                     /locus_tag="VGE92_10840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004629 - phospholipase C activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase C, phosphocholine-specific"
                     /protein_id="HEY1314367.1"
                     /translation="MLARAALRTVKSLERRKFLRSVGAVGAVSLMPSVIRRALAIAAQ
                     VETGTIQDVKHIVILMQENRGFDHYFGTLSGVRGFGDRFPITLPSGQPVWSQSTGSAI
                     IPPYHLDPTTTSALLVPDTPHSFSDAQAAWNQGIMSEWPRFKTGYSMGHYARADIPFQ
                     FALAEAFTVCDAYHCSITGGTDPNRVAFFSGSNFNPTLGQSGVNCTAAQAEIDNTRCT
                     VTGTMPTPGYTYQGSAFQWATLPDILQKAGISWRIYQNPNSNYNGGMNGCLAFQSFRD
                     ATVNSGSPLYANGMSLFTPADLLDDVMNGTLPAVSWILPTATQCEHPSVSSPPTGADF
                     TAQILSAVTANPEVWAQTVFFLVYDENDGFFDHVPPPAVPSYNADGTLAGASTLPLAG
                     EYFSDPTRANLNPNDTISGTVRPWGLGPRVPMCVISPWSRGGWVNSQVFDHTSMGMFL
                     EQRFGISVPSISPWHRAVCGDLTSNFDFATPNDALLPALPNVSNYAAIVAQQKTLPKP
                     VPPALPQPLFQETGTRYSRALPYELHTSARVQAGGGVTLIFSNTGKQAAVFHVYDQLH
                     LERIPRRYTVEAGKMLSDVWQTAATDQGQYNLWVYSANGFFRGFAGNAFLEAAADFTP
                     ETQICYETCSGQVY"
     gene            complement(2177..3625)
                     /locus_tag="VGE92_10845"
     CDS             complement(2177..3625)
                     /locus_tag="VGE92_10845"
                     /inference="COORDINATES: protein motif:HMM:NF001989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEY1314368.1"
                     /translation="MSARARAVMVCGTTSGAGKSFLATALARWYSRQGFKVAPFKAQN
                     MSNNARVVADGEIGSAQYFQALAARAIPHVRMNPVLLKPETATRSQVILLGQRHDELS
                     CTEWHTRAQRLWPTIRGCLDELMAEHDVVVIEGAGSPAEINLSATDVVNMRVAEAAAA
                     ATLLISDIDRGGAFAHLYGTYQLLSAAHRSLIRGFVLNKFRGDLHLLAPGPQMLQALT
                     GISTLAVLPMWREHGLPEEDGLIDHRPSGAGLAVAILAYPCISNLDEFAPLTQASGVS
                     LRWVRDAGSIAGADLLILPGSKNVAGDLAWLRQHELDHAIAAHVAADKPTLAICGGLQ
                     MLGGEIRDPHAVEGRAHGLGLLPYATELRRLKLYRHATHRLTTAQGFWAPLNGIDYPA
                     YEIRHGETRSSEQQSQPLRLRPVLAENRGWQSGQTLAIYMHGLFESPQIMRALFGQET
                     RTLEDTFDGLADFIDARFAQGTLSSLLGAAGA"
     gene            complement(3622..4173)
                     /locus_tag="VGE92_10850"
     CDS             complement(3622..4173)
                     /locus_tag="VGE92_10850"
                     /inference="COORDINATES: protein motif:HMM:NF016180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HEY1314369.1"
                     /translation="MVESAARTLPAIAARPWLKALPPALRLVFQGDAAARSAAAAAWG
                     VAFSEEACRAVVGDGRATLWLGPDEFLLLAADPDSAGSTLAALEQALGTTPHALVDVS
                     HRQFALEISGPHAAAILNGACPLDLDLDEFPLGMCTRTVLSKADIVLWRTRADAFHLE
                     VWRSFGGYVTGLLREIALEFYGE"
     gene            complement(4166..7168)
                     /locus_tag="VGE92_10855"
     CDS             complement(4166..7168)
                     /locus_tag="VGE92_10855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit alpha family protein"
                     /protein_id="HEY1314370.1"
                     /translation="MSATQNEWRTPDGGRVDRARRVEFKFDGQTYQGLAGDTLASALL
                     ANGVHLTGRSFKYHRPRGIVAAGSEEPNALVTVCRDAARTTPNLRATQVELYEGLIAE
                     SQNRWPSLRFDVGRINDRLSAFFPAGFYYKTFMWPKKAWRSLYEPLIRRAAGLGRAPT
                     QPDPDRYAQRYAHCDVLVVGAGPAGLAAAAAAAASGARVILCDESAEFGGSLLSDVHA
                     RIDGEPALGWLRQCLNVLENNSRVMLLTRTTAFGYFPHNLIGLNQRLHDHLARPAGNQ
                     PRERLWQVRARSVVLATGAIERPLVFPGNDRPGILLAGAAHTYLNRYGVRAGSRAVVV
                     TCTDAAYETALDLKAAGVAIAAIADLRSEVAGALPDAARRAGLDVRPGSTVLGTEGDL
                     HVGAITLGRVGSAGVEPGPRIPCDLVLMSGGFTPSVHLFSQSRGKLEWDESLEAFVPA
                     ASAENERSAGACRGVYDLLDVLNDGAAAGAAAAPQAAHTPPRFTVDAARRSAGAYLGV
                     LPQAASPSSDKSFVDWQHDVTARDLALATREGFRSIEHVKRYTTTGMATDQGKTSNLN
                     AMAIVAKALDVAVPQVGLTTFRMPYTPVTFGSMAGISRGDLFDPVRTTPTHAWAQAQG
                     AIFENVGLWKRARYFPRDGEDMHAAVARECRAVRSACGLFDASTLGKIEVVGPDAAEF
                     MNRLYVNNWSNLAVGRSRYGILLREDGFIFDDGVVARTGTDRFHVTTTTGGAPRVLAL
                     MEDYRQTEWPELKVWLTSTTEQWAVIAVQGPNARQVLEPLVEGLDISAATWPHMSVAR
                     GRICGVPMLLFRVSFTGELGFEINVPSDYGLKVWEAVHAAGLAHGITAYGTETMHVLR
                     AEKGYIIVGQDTDGTVTPDDAGLAWAVGKNKRDFVGKRSLERASMKAPDRKQLVGLRT
                     KEPATVLEEGAQVAAIPGQRPPMQLIGHVTSSYASSVLGHSIAMAVIAGGRARLGQTL
                     SVPMPDRDIPVEVVSAVFYDPSGARING"
     gene            complement(7165..7455)
                     /locus_tag="VGE92_10860"
     CDS             complement(7165..7455)
                     /locus_tag="VGE92_10860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEY1314371.1"
                     /translation="MLVIVCPYCGKRPEIEFSYGGQAHLARPSQPAQLDDQAWAEFLY
                     MRTNTKGVYAERWRHARGCGRFFNALRDTSTDHFLAFYKTGDAQPVLPERAP"
     gene            complement(7466..>8222)
                     /locus_tag="VGE92_10865"
     CDS             complement(7466..>8222)
                     /locus_tag="VGE92_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008365064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1314372.1"
                     /translation="RYPVLGAALQRRGGTARHDAVAWGYARAADALGVDIIENCEVTG
                     IRRGGGGGVEALETTRGVIGARKVGVSAAGHTSVIMRMAGVHVPLESFPLQALVSEPI
                     KPIFPCVVMSNTIHAYISQSDKGELVIGAGTDAYTSYTQRGALHVNTHTLDAICELFP
                     NFRRLRMLRNWGGIVDVTPDRSPIIAKTPVPGLYVNCGWGTGGFKATPGSAHVFAWTI
                     ARDEPHPINAPFTIERFRDGYLIDEAAAAAVAH"
BASE COUNT         1496 a         2578 c         2722 g         1426 t
ORIGIN      
        1 atatagacct gcccggagca ggtttcatag cagatttgag tttccggagt gaaatccgcg
       61 gccgcttcca ggaacgcgtt gcccgcgaat ccgcgaaaga agccgttcgc tgaataaacc
      121 cataggttgt actgaccttg atccgtggcc gcggtctgcc aaacatcgga cagcattttg
      181 ccggcctcca ccgtgtagcg gcgcgggatc cgctcgaggt gcaactgatc atagacatgg
      241 aacaccgcgg cttgctttcc cgtgttgctg aagatcaagg tgacgccgcc gcccgcctgt
      301 acgcgggcgc tggtgtgcag ttcatagggc agtgcgcgcg agtaacgcgt gccggtttcc
      361 tgaaacaagg gctgcgggag ggcgggcgga accggtttgg gcagagtctt ttgctgcgcg
      421 acgatcgcgg cgtaattact gacgttgggc agggcgggca gtagcgcatc gttcggcgtc
      481 gcgaagtcga agttggaggt caggtcgccg caaacggccc gatgccacgg gctgatgctc
      541 ggtaccgaaa tcccaaagcg ctgttccaag aacatcccca tcgaggtgtg atcgaacacc
      601 tgcgaattga cccatccgcc gcggctccag ggggagatga cgcacatcgg tacgcgcggt
      661 ccgagtcccc acggccgcac ggtcccgctg atcgtgtcat tgggatttag attcgctcgg
      721 gtgggatccg agaagtattc gcccgccaaa ggtagggtcg aggcgccggc gagagtgccg
      781 tcggcgttgt acgacgggac tgcgggcggc ggcacatggt cgaagaagcc gtcgttctcg
      841 tcgtaaacca agaaaaatac cgtttgcgcc cacacctcgg gattggccgt cacggcgctc
      901 aagatctgcg ctgtgaagtc cgctccagtc ggcggcgaag acaccgacgg atgttcgcac
      961 tgcgtcgccg tgggcagtat ccacgatacg gcgggcagag ttccgttcat cacgtcatcg
     1021 agtagatctg ccggtgtgaa cagcgacatc ccgttcgcgt agagaggcga gcccgagttc
     1081 acggttgcgt cgcggaagct ttgaaatgcg agacagccgt tcatgccgcc gttgtaattg
     1141 ctgttcgggt tctgatagat gcgccaactg atgcccgcct tttgcagaat atccggcagg
     1201 gtggcccatt gaaacgccga gccctggtag gtatagcccg gcgtcggcat cgtgccggtc
     1261 accgtgcagc gcgtattatc gatctcggcc tgcgccgcag tgcagttgac gccggattgc
     1321 ccgagcgtcg gattgaagtt cgaaccggag aagaaagcga cgcgattcgg atcggtgccg
     1381 cccgtgatcg agcagtggta ggcatcgcag actgtaaagg cttcggccag cgcgaactgg
     1441 aaaggaatgt cggcgcgcgc atagtggccc atcgaatacc cggtcttgaa gcgcggccat
     1501 tcgctcatga tgccttgatt ccatgccgcc tgcgcgtcgg aaaaactgtg cggcgtgtcc
     1561 ggcacgagca gggcgctggt ggtggtcggg tccaaatggt agggcgggat gatcgcgctc
     1621 cccgtggact gagaccagac cggctgaccg ctcggcagcg tgatgggaaa gcggtcgcca
     1681 aagccgcgta cgccgctcaa ggtgccgaag tagtgatcga agccgcggtt ctcctgcatc
     1741 agaatgacga tgtgtttgac gtcctggatg gttccggtct cgacctgcgc ggcaatcgcg
     1801 agcgcgcgcc gaatcacgct tggcatgaga ctcacggcac cgaccgcgcc tacggatcgc
     1861 aggaattttc gccgctcgag actcttcacg gtgcgcagtg cagctcgggc gagcactgtg
     1921 ccgtcgtggt cgatgtttgg gtggattgac ttgcgccagc actttgaccg cccgcgctgc
     1981 cgccgcctcc gcaggcaacc aaactcatga gcagcgcagc cgcgccgaga tggcaagtca
     2041 aggtcgttaa ggatttgtaa tccgtgcaac ggcggcacgc gcgagcggca tacatcgccg
     2101 tgcgcaaacc gaatgaatct accaccattg tattatgtcc actccctgtg aattgaccgc
     2161 tgaagcgcgg ctaagttcaa gcgccggcgg cgccaagcag agacgagagt gtaccttgag
     2221 caaaccgcgc gtcaatgaaa tcagccaagc cgtcgaaggt atcctcgagc gtgcgcgtct
     2281 cttgtccaaa taatgcgcgc atgatttgcg ggctctcgaa taagccatgc atataaatgg
     2341 cgagcgtctg gcccgattgc cagccgcgat tttcagcgag cacggggcgc aggcgtagcg
     2401 gctgcgattg ctgctcgctc gagcgcgttt ctccgtggcg aatctcgtac gccgggtaat
     2461 cgattccgtt cagcggcgcc cagaatccct gcgccgtcgt gagcctatgg gtcgcatggc
     2521 gatacagctt caagcgccgc aactcggtcg cgtagggcag aaggccgagg ccgtgcgctc
     2581 gcccttcgac cgcgtgcggg tcgcggattt cgccaccgag catttgcagc ccgccgcaga
     2641 tcgcgagcgt cggtttgtcg gccgctacgt gtgccgcgat cgcgtgatcg agttcatgtt
     2701 gtcgcagcca cgcgagatcg ccggcgacgt ttttcgatcc cggcaggatc aacagatcgg
     2761 cgcccgcaat gctgcccgcg tctcggaccc agcgaagcga cacgccggac gcttgagtca
     2821 acggcgcgaa ctcgtccaga ttgctgatgc atggataagc gaggatcgca accgcgagtc
     2881 ccgcgccgct cggccggtgg tcgatcaggc cgtcttcctc cggcagacca tgctcgcgcc
     2941 acatgggcaa caccgccagc gtggaaattc cggtgagtgc ctgcagcatc tgcggtcccg
     3001 gcgccaacaa gtgcaaatcg ccgcgaaact tgttgaggac gaagccgcga atcaaggagc
     3061 ggtgggcggc actcagcaat tggtaagtgc cgtaaagatg ggcgaaagcg ccgcctcgat
     3121 cgatgtcgct gatgagcagc gtggcggccg cggccgcctc ggcgacgcgc atattgacga
     3181 catcggtcgc gctcaagttg atctccgccg gcgaaccggc gccctcgatc acgacaacgt
     3241 cgtgctccgc catgagctca tcgaggcagc cgcggatcgt tggccaaagc cgctgcgcgc
     3301 gcgtgtgcca ctccgtgcac gagagttcat cgtgccgttg gccgagcaga atgacttggc
     3361 tgcgagtcgc cgtctcgggc ttgagcagca cggggttcat gcgcacatgc ggaatcgccc
     3421 gagccgccaa cgcctgaaag tattgcgcgc tgccgatttc accgtccgcc acgacgcgcg
     3481 cgttgttgct catgttctgc gccttgaacg gcgcaacctt gaatccctgg cgtgaatacc
     3541 agcgcgccag ggcggtggcg agaaaactct tgcccgcgcc gctggtcgtg ccgcacacca
     3601 tgacggcacg agctcgcgcg ctcactcgcc gtagaactcc agcgcgattt cacgcagcaa
     3661 cccggtcaca tagccgccga aggagcgcca tacttcaaga tgaaaggcgt cggcgcgcgt
     3721 gcgccaaagc acgatgtcgg ccttggagag aacggtgcgg gtgcacatac ccagcggaaa
     3781 ttcatcgaga tccaaatcga gcggacaggc gccgttcaaa atggcagccg catgcgggcc
     3841 gcttatttcg agcgcgaatt gccgatgact cacgtccacg agcgcgtgcg gtgtcgtgcc
     3901 gagcgcctgc tcgagagcag caagggtgct gccggcggaa tcggggtcgg cggccaacag
     3961 cagaaattca tcgggaccga gccacagagt cgcacggcca tcgccgacca cggcgcggca
     4021 tgcctcctcc gagaaggcga cgccccaggc ggcggcagcc gccgaccgcg ccgcggcgtc
     4081 accctgaaaa acgagccgca gcgccggcgg cagcgccttg agccaggggc gcgcggcaat
     4141 cgccggcaag gtgcgtgcgg cggattcaac cattgatgcg tgctccgctc ggatcgtaga
     4201 acacggcgga caccacttcg acggggatgt cgcgatcggg catcggtacg ctgagcgtct
     4261 gtccgagtcg ggcgcgcccg cccgcgatga ccgccatggc aatcgaatgt ccaagcacgc
     4321 tgctggcgta ggaggaggtc acgtgaccga tcagttgcat cggcggcctc tggcccggga
     4381 tcgcggcgac ctgcgcgcct tcttcgagca ccgtagcggg ttccttggtg cgcagcccaa
     4441 ccagctgctt gcgatcgggc gctttcatcg aggcgcgttc gagcgagcgc ttgccgacaa
     4501 aatcgcgctt gttcttgccg accgcccagg caagtccggc atcatcgggg gtcaccgtgc
     4561 catcggtgtc ctggccgacg atgatgtagc ccttctccgc gcgcagtacg tgcatggttt
     4621 ccgtgccgta cgccgtaatg ccgtgcgcca ggccggccgc gtggaccgcc tcccaaacct
     4681 tcagaccgta atccgacggc acgttgattt cgaatccgag ctcacccgtg aagctgacgc
     4741 gaaacagcag catcggcacg ccgcaaatgc ggccgcgcgc gacgctcatg tgcggccagg
     4801 tggcggcgct gatgtcaagg ccctcgacca agggctcgag cacctgacgc gcgttcggtc
     4861 cctgcactgc aatgaccgcc cactgctcgg tagtcgaggt cagccagacc ttgagctccg
     4921 gccactctgt ctgacgatag tcttccatca gcgcgagaac gcgcggcgca ccgccggtgg
     4981 tggtggtgac gtggaagcgg tcggtgccgg tgcgtgcgac gacgccgtca tcgaagatga
     5041 agccgtcctc acgcagcaat atcccgtaac gcgagcggcc gactgcgagg ttggaccaat
     5101 tgttgacgta caagcggttc atgaactcgg cggcgtccgg cccgacgacc tcgatcttgc
     5161 cgagtgtcga ggcgtcgaat aagccgcagg cgctgcgcac cgcgcggcac tcgcgcgcca
     5221 ccgcagcgtg catgtcttcg ccgtcgcgag gaaaataacg ggcgcgcttc cacaaaccga
     5281 cgttctcgaa tatcgcgccc tgcgcctgcg cccaggcgtg cgtcggtgtg gtgcgcaccg
     5341 ggtcgaacaa atcaccgcgt gaaatgcctg ccatgctgcc gaaagtcacc ggcgtgtagg
     5401 gcatgcggaa cgtggtaaga cccacttgcg gtacggcaac gtcgagcgcc ttggcgacaa
     5461 tggccatggc gttcagattc gaagtcttgc cttgatcggt cgccatgccc gtcgtggtgt
     5521 agcgcttgac gtgttcgatc gaccggaagc cctcgcgcgt ggcgagcgcc agatcgcgcg
     5581 cggtcacatc gtgctgccaa tcgacgaagg acttatcgct gctgggtgag gctgcttgcg
     5641 gcaatactcc gagataggcg ccagcgctgc gcctcgcggc gtccaccgtg aagcggggcg
     5701 gcgtgtgagc ggcttgcgga gcggcagccg cgccggcggc ggcgccatca ttgagcacat
     5761 cgaggagatc gtagacaccg cggcaggcgc ccgccgaacg ttcgttctca gcggaggcag
     5821 ccggcacaaa agcctcgagg ctctcatccc actcgagctt gccgcgggat tgcgaaaaca
     5881 agtgcacgct cggagtgaag cccccggaca tgagcaccaa atcgcagggg attcgcggtc
     5941 cgggttcgac gcccgcgctg ccaacgcggc cgagcgtgat ggcgccgacg tgcaaatcgc
     6001 cttcggtgcc gagcaccgtc gagccaggcc gcacgtcgag gccggcgcgc cgcgcggcat
     6061 cgggcagcgc gcccgcgacc tccgatcgta aatcggcgat ggccgcaatg gcgactccag
     6121 cagccttgag gtcgagagcc gtctcatacg cagcgtcggt gcacgtcacg acgacggcgc
     6181 ggctgccggc gcgcactcca tagcggttga gatacgtgtg cgccgcgccg gcgagcaata
     6241 tcccgggccg atcgttgcct ggaaacacca gcgggcgttc gatcgctccg gttgcgagca
     6301 cgacggagcg ggcccgaacc tgccagagcc gttcgcgcgg ctgattgccg gcgggtcgcg
     6361 ccagatgatc atgcagccgt tgattcaggc cgatgagatt gtgcggaaaa tagccgaacg
     6421 cagtggtgcg ggtcaggagc atcacgcggc tgttgttttc gaggacgttg aggcactgcc
     6481 gcagccagcc gagcgcgggc tcaccgtcga tccgcgcatg gacatcgctc agcaggctgc
     6541 cgccgaactc ggcgctctca tcgcacagga tcactcgcgc accgctcgcg gccgccgcag
     6601 ctgcggcggc aagcccggcc ggacccgcgc cgaccacgag cacatcgcaa tgcgcatagc
     6661 gctgcgcata gcgatccgga tcgggttgtg tcggtgcgcg gcccaggccc gcggcgcggc
     6721 ggatcagcgg ttcgtacagg gaccgccacg cctttttcgg ccacatgaaa gtcttgtagt
     6781 agaaacccgc ggggaagaat gccgacagtc gatcgttgat gcggccgacg tcgaatcgca
     6841 agctgggcca gcgattctgg ctttcggcga tcaagccctc atacagctcg acttgagtgg
     6901 cgcgcaggtt cggtgtggtg cgcgcggcgt cccggcatac cgtcaccaac gcattgggtt
     6961 cctcggatcc ggcggcgaca atgccgcgcg gccggtgata tttgaacgag cggcccgtca
     7021 agtgcacgcc gttcgcgagc agtgccgatg ccagcgtgtc gcccgcaagc ccctgatagg
     7081 tttggccgtc gaatttgaat tcgacacggc gcgcacggtc cacccggccg ccgtccggcg
     7141 tgcgccattc gttttgcgta gcgctcacgg cgcgcgctcg ggcagcaccg gttgtgcgtc
     7201 gccggttttg tagaaggcga gaaaatggtc ggtcgaagtg tcgcgcagcg cgttgaaaaa
     7261 acggccgcaa ccgcgcgcat gacgccagcg ttcggcatat acgcccttgg tgttggtgcg
     7321 catgtacaga aattccgccc atgcttggtc gtcgagctgc gcgggttggc ttggccgcgc
     7381 cagatgcgcc tggccgccgt aggagaactc gatttccgga cgcttaccgc agtaggggca
     7441 gacgatgacg agcattgggg ctccgttaat gagcgaccgc ggctgccgcg gcttcgtcga
     7501 tgagatagcc gtcgcgaaag cgttcgatgg tgaacggcgc gttgatgggg tgcggttcgt
     7561 cgcgtgcaat ggtccaggcg aagacatgcg ccgatccggg ggttgctttg aatccgccgg
     7621 tgccccaccc gcaattgacg tataagccgg gcaccggggt cttggcgatg atcggtgaac
     7681 gatccggcgt tacatcgacg atgccgcccc agttgcgcag catccgcaga cggcgaaagt
     7741 tggggaacaa ctcgcagatc gcgtccagcg tatgcgtatt gacgtgcaac gcgccgcgtt
     7801 gggtatacga ggtgtaggca tcggtgccgg cgccgatcac cagttccccc ttgtccgact
     7861 ggctgatgta ggcgtggatg gtattggaca tgaccacgca tggaaaaata ggcttgatgg
     7921 gttccgaaac cagcgcttgc agcgggaagc tctcgagcgg cacgtggaca cccgccatgc
     7981 gcatgatgac gctggtgtgt ccggcggccg acacgccgac tttgcgcgcg ccaatcacgc
     8041 cgcgggtggt ttcgagtgcc tccacgccgc cgccgccgcc gcgccggata cccgtcacct
     8101 cgcaattctc gataatgtcg acgccgagcg cgtcggccgc ccgtgcgtag ccccaggcaa
     8161 cggcgtcgtg ccgcgcggtg ccgccgcgcc gctgcagagc ggcgcccagc accggatagc
     8221 gg
//
LOCUS       DASWZT010000385         2909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_75487, whole genome shotgun sequence.
ACCESSION   DASWZT010000385 DASWZT010000000
VERSION     DASWZT010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75487"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="VGE92_10870"
     CDS             <1..1086
                     /locus_tag="VGE92_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005264012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3362 domain-containing protein"
                     /protein_id="HEY1314373.1"
                     /translation="AYGSAKIPAYHMIRFSVAIQRGCFGGCTFCSITEHEGRIIQSRS
                     EDSVIREIETIRDRVQGFTGVISDLGGPTANMYRLHCKSPAIESACRRPSCVFPGVCP
                     NLNTDHAPLINLYRRARALKGIKKVLIASGVRYDLAIESPEYVRELATHHTGGYLKIA
                     PEAIGEGPLSKMMKPGVGAYYRFKELFDKYSKEAGKEQYLIPYFIAAHPGTRDQDMLE
                     LALWLKQNGFRADQVQAFLPSPMATATAMFHSGKNPLRRITRSSEEVFIPKGIKVRRL
                     HKAFLRYHDPDNWPVLREALKRMGRADLIGNGARHLIPAYQPVARGGARAATLPFRTQ
                     HTGLPKSGGANAGKPGRGSGHLRRGRP"
     gene            1223..>2909
                     /locus_tag="VGE92_10875"
     CDS             1223..>2909
                     /locus_tag="VGE92_10875"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEY1314374.1"
                     /translation="MDNWIVHKFGGSSVADASCFRRVADIVEASPNPREAVVLSACRG
                     VTDALLALVTLAEKPDRDFAPAIEALKQRHRDLAASLVSKEACERYNTQLERDCADIA
                     GMLQTVRLIRSSTYSMRDVISGYGEIWSTRLFAPFLRERGRIKGDVLWIDAREVVIIE
                     WGSLGPAVQWAVSEANLKRLVPQVFTGRLIVTGFVASTVKGNQTTLGRNGSDFSGSIF
                     GALLNASEIIIWTDVDGVLSADPRLVPNAQVIDQLSYNEAMELAYFGAKVIHPQTMEP
                     AVARDIPIYIRNTFAPQKHGTLICAHPKSALKVKGITTIDPVALVNLEGAGMIGVPGT
                     AHRLFGALRDAGISVILISQGSSEHSICFAIPQAQAVRAEEAVRRAFDVELRDGQIQR
                     VEVALGMSILAVVGDGMAGAHGVAAKVFNSLGAAAINVRAIAQGASERNISVVVDGKV
                     GAKALRAVHAAFYLSPNTLSIGLIGPGTVGRVLLAQIGTQIERLRALNLDLRVRGIAT
                     STRMLLEESAIDLDHWAERLAAAGEPLDLEKFANHVQADYIPHTAIIDCTASVE"
BASE COUNT          517 a          895 c          953 g          544 t
ORIGIN      
        1 gcctacggca gcgcgaaaat tcccgcctat cacatgatcc gattctcggt ggcgatccag
       61 cgcggctgtt ttggcggctg caccttttgt tcgatcaccg agcatgaggg gcgcatcatc
      121 cagagtcgct ccgaggattc cgtgatccgg gagatcgaaa ccatccgcga ccgggtgcag
      181 gggtttaccg gcgtcatttc cgatctcggc ggcccgacgg ccaacatgta ccgcctgcat
      241 tgcaagagcc cggcgataga gtccgcgtgc cgccgcccgt cgtgcgtgtt tccgggcgta
      301 tgcccgaatt tgaataccga ccatgcgcca ttgatcaacc tatatcggcg cgcgcgggca
      361 ttgaagggaa tcaaaaaagt gttgattgcc tccggcgtgc gctatgactt ggcgatcgag
      421 tcgcccgagt acgtgcgcga actcgcaacc caccacaccg gcgggtatct gaaaattgcg
      481 cccgaggcca tcggcgaggg gccgctctcg aagatgatga agcctggggt cggcgcgtac
      541 taccgcttca aggagctgtt cgacaaatat tcgaaggagg cgggcaagga acagtacttg
      601 attccttatt tcatcgccgc tcatcccggc acccgcgatc aggacatgtt ggaacttgcg
      661 ctgtggctga aacagaacgg atttcgcgcc gatcaggttc aggcattcct gccctcgccg
      721 atggctacgg ccaccgccat gtttcactcg ggcaagaatc cgttgcggcg cattacccgc
      781 agcagcgaag aggtcttcat cccgaagggg atcaaggtgc gccgcttgca caaggcgttt
      841 ctgcgatatc acgatccgga caattggccg gtgctgcgcg aggcattgaa gcgcatgggc
      901 cgcgccgact tgatcggcaa cggcgcgcgc cacctgattc cggcctacca accggtggcg
      961 cgcggcgggg cgcgggccgc gaccctgccg tttcgcaccc agcacacggg attgcccaaa
     1021 tcggggggcg cgaacgccgg caagccgggc cgcggcagcg gccatctgcg tcggggtcgg
     1081 ccctaaagcg aacggaatcg cttgcttggc tccccgcggt gcgattaaag ttcgggctgc
     1141 ctagcggttt cctaccgcaa ttcagtaact tataagattt gggagcggta aggcgcccgc
     1201 gcgatgcggg cccgtgtgaa gcatggacaa ttggatcgta cataagtttg ggggctcgag
     1261 cgtcgccgac gcgagctgtt ttcgccgggt ggcggacatc gtcgaggcat cgcccaatcc
     1321 gcgcgaagcg gtggtgctgt ccgcctgccg cggggtgacg gatgcgttgc tggccttggt
     1381 cacgctcgcg gaaaaaccgg accgcgattt cgcgccggcg atcgaggcgc tcaagcagcg
     1441 ccaccgcgat cttgcggcat cgttggtctc caaagaggcc tgcgagcgct acaacacgca
     1501 actcgaacgc gattgcgccg atatcgccgg catgctgcag acggtgcgcc tgatacggtc
     1561 ctccacgtat tcgatgcgcg atgtcatttc cggctacggc gagatctggt cgacgcgcct
     1621 gttcgcgccg ttcctgcgcg agcgcggccg catcaagggc gatgtgctgt ggatcgatgc
     1681 gcgtgaagtg gtcatcatcg aatggggatc gttggggcct gccgtgcaat gggcggtgag
     1741 cgaggcgaac ttgaagcggc tggtgccgca ggtcttcact ggccgcttga tcgtgaccgg
     1801 attcgtcgcc tccacggtga aaggtaatca gaccaccctc gggcgcaacg gcagcgactt
     1861 ctccggctcc attttcggtg cgctgctcaa tgcctcggaa atcatcattt ggaccgatgt
     1921 cgacggcgtg ctgtcggccg atccgcggct ggtgccgaac gcccaggtga tcgatcagct
     1981 gtcgtacaac gaggccatgg agctcgcgta cttcggcgcc aaggtgatcc atccacaaac
     2041 catggagccg gcggtcgcgc gcgatattcc gatttatatc cgcaacacct tcgcgccgca
     2101 gaagcacggc accctgattt gcgcgcaccc caagtcggca ctcaaggtga agggcatcac
     2161 caccatcgat ccggtggcgc tggtcaatct cgagggggct ggaatgatcg gcgttcccgg
     2221 taccgcgcat cgtttgttcg gcgcgctgcg cgatgcgggc atctcggtga tcttgatttc
     2281 gcaaggcagt tcggagcact ccatttgctt cgccattccg caggcgcagg cggtgcgtgc
     2341 cgaggaggcg gtgcggcgcg cgttcgacgt ggaactgcgc gacggacaaa ttcagcgcgt
     2401 cgaagtcgct ctcggcatga gcatcctggc ggtggtcggg gacggcatgg ccggcgcgca
     2461 cggcgtcgcc gccaaggtat tcaattcgct cggtgccgcc gccatcaacg tgcgtgcgat
     2521 cgcacagggg gcctccgagc gcaatatttc ggtggtggtg gacggcaaag tgggcgccaa
     2581 ggcgctgcgc gccgtgcacg cggcgttcta cctgtcgccg aacacgctgt cgatcggctt
     2641 gatcgggccg ggcaccgtcg gccgggtgct gttggcgcag atcggcacgc agatcgaacg
     2701 cttgcgggcg ctcaacttgg atctgcgggt gcgcggcatc gccacctcca cccgcatgct
     2761 gctcgaggag agcgcaatcg acctcgacca ctgggccgag cggctggccg ccgcgggcga
     2821 gccgctcgat cttgaaaaat tcgccaatca cgtgcaagcg gactatatcc cgcacacggc
     2881 gatcatcgac tgtacggcga gcgtcgagg
//
LOCUS       DASWZT010000386         3034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_70775, whole genome shotgun sequence.
ACCESSION   DASWZT010000386 DASWZT010000000
VERSION     DASWZT010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70775"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1373
                     /locus_tag="VGE92_10880"
     CDS             120..1373
                     /locus_tag="VGE92_10880"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY1314375.1"
                     /translation="MKFYGLALQSRLTSWAILASLLALAACDDNHHHYACINCGATAT
                     PYEVSLGLIAGNFTNNGDTSLIATSTILYNPQVNAGNLKTYLSSGPGAFAAPVLIADG
                     DDPLYLATADLNGDKLPDVVSASYSDGTLSVFFNSAQSPGTFSTPLVLSSPGASQVAI
                     ADMNGDGLPDIVSADFNVSLFLQTSPGAFASPVSLYSGGANWVAVGDLNGDGAPDVAL
                     TDNVGVKILLHTGAASATTFAAPVTVFTQTVNANAVGANLIAIADVNGDGLNDLVITD
                     PGPTGGAAPTVSVLLQDPANHGQFLAAVSYPIAPGSVPTSIIVTDVNGDNHPDIVIGG
                     STAVSVLLQVAATPGTFAAATNYAVTNANQIAIADVNGDGHLDIVVGTGVTHTLQSGV
                     VSNNPGVLLQSAASPGTFGSLQDLP"
     gene            complement(1382..2707)
                     /locus_tag="VGE92_10885"
     CDS             complement(1382..2707)
                     /locus_tag="VGE92_10885"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1314376.1"
                     /translation="MPKTFSPVDESTAYPRRTYAWCVVAILIGTAIVSYTDRQVLSLL
                     VDPVRHDLGISDTQISLLLGTAFAVVYGIAGIPFGLLADRTSRRNLIFCGAVVWSIGT
                     VACGYVHSFGELFAARIVVGLGESVLSPAAISLISDYFPPARRGLAVGCFLSGIAIGI
                     GASILIGGGVLHLVEVGAFAGTPFARMVPWRLVLLLIGALGLAWSFMILLIQEPKRRV
                     TEPPAVPDALPRRRVLLSMVAPIYVVVATASLVDNAVGAWAPTLLIRNFGRDPAQVGL
                     QLGLLLTIAFGGGVLLGGWLSDWVGGRGGVKAKLGVCLISSLLILPVALAIDSSQLAV
                     VMAAVPLYFALSGIVTACGFSSILDATPNLQRGFAMAVSFFLNVAVGAGVGPTSVALA
                     STHVFGAQAGLGPAIAFVAGFAYLLAAAALVLTRLAFKLAQRPQGQFAG"
     gene            complement(2729..>3034)
                     /locus_tag="VGE92_10890"
     CDS             complement(2729..>3034)
                     /locus_tag="VGE92_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314377.1"
                     /translation="SLNYRQPVNEQLNLVARVTNSYVDSIQDITFTRNSLPPYDLVGF
                     RLGVENAKWSAYLFADNLTNKMALLSDTGALSANISILNRIATNQPRTVGADLSFKF"
BASE COUNT          615 a         1026 c          887 g          506 t
ORIGIN      
        1 gctcgtatcg cgaacgcctt gtaataacta caattcatac cgtcaacgga atcggcgctc
       61 attcgttttt gcagttgcgg ccaacgcgcg cgtgtagttc catgccctgg aggttgtata
      121 tgaaattcta tggtctggcg ctccaatcgc gcttaacgtc gtgggcaatt ctcgcatcct
      181 tgctggcact cgccgcttgc gatgacaatc atcaccacta cgcatgcatc aactgcggcg
      241 ctaccgcgac gccctacgag gtgtcgttgg gactcatcgc cggaaatttc acgaacaatg
      301 gtgacacctc gctcatcgcg accagcacca tcctctacaa cccgcaagtc aatgccggta
      361 atctcaaaac ttacctgtcg agcggccccg gtgcctttgc cgccccggtg ctcatcgccg
      421 atggcgacga cccgctgtat ctcgccaccg ccgatctcaa cggcgacaaa ctgcccgacg
      481 tcgtctccgc cagttacagc gacggcacgc tctccgtatt cttcaacagc gcgcaaagtc
      541 cggggacctt cagcacgccc ctggtgctgt catccccggg cgcctcccag gttgcgatcg
      601 cggacatgaa cggcgacggc ctgcctgata tcgtgtcggc cgatttcaat gtgtcgctgt
      661 ttctgcaaac ctctcccggc gcatttgcga gtcccgtcag cttgtattcg ggcggcgcga
      721 actgggtcgc agtcggcgat ttgaatggcg acggcgcgcc cgatgtggcc ttgacggata
      781 acgtcggcgt caagatcctg ctgcataccg gagctgcgag cgcgacgacc ttcgcggccc
      841 cggtgaccgt attcacgcaa acggtcaacg ccaacgcggt gggcgcgaat ctcattgcca
      901 ttgccgatgt gaatggcgac ggcttgaacg acctcgtgat caccgacccc ggccccacgg
      961 gcggcgccgc acccacggtg tcggtgctgc tacaggaccc cgcgaaccac ggccaattcc
     1021 tcgccgccgt gagctatccc atcgcgcccg ggagtgttcc gacctcgatc atcgtgaccg
     1081 acgtcaacgg cgacaatcat ccggatatcg tgatcggcgg cagcaccgcg gtcagcgtgc
     1141 tgctgcaggt cgcagccacg cccggtacct tcgcggcggc gaccaactac gccgtgacga
     1201 acgccaatca aatcgcgatc gcagacgtca acggcgatgg tcatctcgat atcgtcgtcg
     1261 ggaccggcgt cacgcatacg cttcagagtg gcgtggtctc gaacaaccca ggcgtgttgc
     1321 tgcaaagtgc ggcttccccc ggcaccttcg gctcgctcca ggatctgccc tagcgtcgcg
     1381 ctcagcccgc gaactgccct tgcggtctct gtgcgagctt gaacgcaagg cgcgtgagca
     1441 cgagcgccgc ggccgcgagc aggtacgcga acccggcgac aaatgcgatc gcgggaccta
     1501 atcccgcttg agcgccgaac acatgtgtgc tcgcgagcgc cacggaggtg ggtccgaccc
     1561 cggcgcccac cgccacgttc aagaaaaagc tcaccgccat cgcgaagccg cgctgcaaat
     1621 tcggggtcgc atcgagaatc gacgagaagc cgcacgccgt gacgattccg gacagagcga
     1681 agtacaacgg cacggcggcc atcaccaccg cgagctgcga ggagtcgatg gccaaggcca
     1741 cgggaaggat caacagactc gagatcaggc acacgccgag cttggccttt acgccgccgc
     1801 gcccgccgac ccaatcggac agccacccgc cgagcagcac gccgccgcca aaggcgatgg
     1861 tgagcaacag gccgagctgc agccctactt gcgcgggatc gcgtccgaag ttgcgaatca
     1921 gcagcgtcgg cgcccatgcc ccgacggcat tgtcgaccag cgaggcggtc gccaccacga
     1981 cgtaaatcgg cgccaccatc gacagcagca cgcggcgccg cggaagcgca tcgggcaccg
     2041 ccggcggttc cgtgactcgg cgcttgggtt cttgaatcaa caagatcatg aacgaccagg
     2101 cgagacccag tgcgccgatc aacagcagca cgagccgcca gggcaccatc ctcgcgaagg
     2161 gcgtacccgc aaaggcgccc acctccacca gatgcagtac gccgccgccg atgagaatgg
     2221 aggcgccgat gccgatcgca attccgctca aaaagcagcc caccgccagg ccgcggcgtg
     2281 ccggcggaaa ataatcgctg atcagcgaaa tcgccgccgg cgacagcacg gactcgccga
     2341 gcccgaccac gatccgcgcc gcgaacagtt ctccgaagct gtgcacgtag ccgcaggcca
     2401 cggtgccgat gctccacacc accgcaccgc aaaaaatcaa atttcgccgc gaggtgcgat
     2461 cggccaagag gccgaacggg atgccggcaa tgccatagac gacggcgaat gccgtcccca
     2521 acagcaggct gatttgggtg tcgctgatgc cgagatcgtg ccgcaccgga tcgaccagca
     2581 ggctcaagac ttgccgatcc gtatacgaca cgatggccgt gccgatgagg atcgccacga
     2641 cgcaccaggc ataggtgcgt cgtggatacg ccgttgactc atccaccggc gagaacgttt
     2701 tcggcatgcg cgcccgcctg tatgcgaatc agaatttgaa agacaagtct gctcccaccg
     2761 tgcgcggctg attggtcgcg atccggttca ggatcgagat gttcgccgac agggcacccg
     2821 tgtcgctgag caacgccatc ttgttggtca agttgtccgc gaacaggtaa gccgaccact
     2881 tcgcgttctc caccccgagc ctgaatccga ccagatcgta cggcggcagc gaattgcgag
     2941 tgaacgtgat gtcctggatc gaatcgacgt agctattggt gacgcgcgcc accagattca
     3001 gctgctcgtt caccggctgt ctatagttca agct
//
LOCUS       DASWZT010000387        15462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_46662, whole genome shotgun sequence.
ACCESSION   DASWZT010000387 DASWZT010000000
VERSION     DASWZT010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15462
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46662"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VGE92_10895"
     CDS             complement(<1..752)
                     /locus_tag="VGE92_10895"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY1314378.1"
                     /translation="MKAAWMRALGAVLLAAAPLLGARAATTLVDDAAVYPEGPLWKDG
                     RLLYVEYAGPGIKSWDGKRVATYWNGEHCGASGLIVFGRDHLLVACYDANEIVELDAN
                     GKQIRSIDKDAAGKPFIGPNDFTADGSGGVYVSASGVYDLKAPISGTLLHLAAHGGAP
                     LEVANLIHYANGLTLSKDGRYLLVAEMLAGRILSFQVLPGGTLGPRTVWARLQDLAPP
                     TAGADAYNGPDGFKLGPDGNYYIAQNGSGRVLV"
     gene            complement(749..1984)
                     /locus_tag="VGE92_10900"
     CDS             complement(749..1984)
                     /locus_tag="VGE92_10900"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEY1314379.1"
                     /translation="MQVYLVGGAVRDALLGLEVKERDWVVVGSTREELLRLKFREVGR
                     DFPVFLHPDTHEEYALARLERKVAPGYRGFSVEFGPEVSLEEDLARRDLTINAMAQAT
                     DGTLLDPHGGKRDLDARVLRHVSPAFIEDPVRVLRVARFAARFAPLGFHVADETLELM
                     RAMVERREVDALVPERVWQETEKALREPTASEFFKVLRACDALRPIYPELDALFGVPQ
                     PVQWHPEVDTGIHTLMVLDQAALLSRDSRVRFAALVHDLGKGTTPRTEWPRHRGHEER
                     SVSLIEALADRLRLPAEYRDLGIIVARYHGNVHRAFQLRPNTILEFIERADAFRRPER
                     FALALLACEADARGRTGLEHVPYPQRAYLQAARDAAAAIKPSAEDIAAHSGAKIAERL
                     HQRRAQVIAELREHSGVRQ"
     gene            1987..3039
                     /locus_tag="VGE92_10905"
     CDS             1987..3039
                     /locus_tag="VGE92_10905"
                     /inference="COORDINATES: protein motif:HMM:NF021535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEY1314380.1"
                     /translation="MNLSSINATIRAAFGLAVIASCSGGCYLLQSAQGQLALMSQREP
                     ITRIIDRGSTPPALRAQLEQVAAIRDYASHALGLPDNGSYRSYADVGRRYVVWNVVAA
                     AEFSVDAKDWCYPIVGCVAYRGYFVEQRARRYAAGLRAAGFDVAVGGVAAYSTLGHFN
                     DPVLNTMIGWNDVELAAIIFHELTHQLLYVANDSSFNEALATTVEEEGVRRWLRSQGR
                     DADLANHLAEHEHYVKVVELLGATREELRALYASALAPQMLRNRKRAAFAALRSSYAV
                     LKASWGGHAPFDAWFDEDLNNAHLASIATYFNCVPGFERELAARGGDLTAFYARAREL
                     ARFDQEKRDAIVCAAP"
     gene            3249..4163
                     /locus_tag="VGE92_10910"
     CDS             3249..4163
                     /locus_tag="VGE92_10910"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY1314381.1"
                     /translation="MPAAYDRLPLGALRVFEAVATHLNFSLAADALSVTPAAVSQQIK
                     ALEGYIQVPLFRRTGRRVEITEEGLELLPAVRAGLEKLESALQQIKLHRRAGPLQITT
                     LASFLQIWLLPRIRSFRRKYPSVELRFHTSRELVDFSRTTNHIAIRFGHGDYPSLHCE
                     KLLDDWLVPVASPDLIKQYGTIERGAKLAKYPLLESGDEPWSVWSQTGEEAAWLSRAP
                     SIDDSAGLLTAAEEGLGYVLARWTLVTRALHKGTLRLAGKGALPYGSAYYFVCPKNYL
                     TLPKVAQFREWIIAAARDFPRPATADSN"
     gene            complement(4160..5158)
                     /locus_tag="VGE92_10915"
     CDS             complement(4160..5158)
                     /locus_tag="VGE92_10915"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HEY1314382.1"
                     /translation="MRKPYLLFLGDVQDKLSAKTAFGLKDWCAQDVIGEWSLPGCAVT
                     LGLPRLSPREAAARGAGSIVVGIAPTGGALPRHWVAELEAAADAGLDVVSGLHTRLAS
                     FPELVQSAARRGVRLIDVRHSEQQFGAASGRKRSGKRVLTVGTDCAQGKKYTALALAA
                     NLSRRGVAATFRATGQTGIMISGQGIAIDAVIADFIAGAAEQLSPDNAPEHWDVIEGQ
                     GSLFHPAYAGVTLGLLHGSQPDALVLCHDPSRLTIDEYPEFPLPDLRVAIDEYLRAAR
                     LTNRAVRCIGLSINSSGLSAADCSAYFERLTAELGLPVCDPVRSGVDALATALLAL"
     gene            complement(5169..6143)
                     /locus_tag="VGE92_10920"
     CDS             complement(5169..6143)
                     /locus_tag="VGE92_10920"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HEY1314383.1"
                     /translation="MRPLCIAVLGGTGFLGTRLVARLIKDGHRVTVLSRDREQHKHLL
                     VLPGLTLENCDVYQEAQLSERFRGKDVVINLIGILNERGFGGGGFRRAHVELTRGVLL
                     AVRSAGVARLLQVSALKAAVDAPSYYLRTKGEAEQLIRDANFALDWTIFQPSVMFGPG
                     DSFLNRFAALLASIPFIFPLARPNTRFQPVFVDDVIEALLRSLHGGASSRQTCELGGP
                     QVYSLREIVALVGKLTGRRRWIIGLPNVVARTQALVMDFVPGRPFSSDNYRSLKIDSV
                     CTQDGFAKLGLKPQSMLASARQYLGAYEDNARLSHNRASAGRNRPLRS"
     gene            complement(6149..7528)
                     /locus_tag="VGE92_10925"
     CDS             complement(6149..7528)
                     /locus_tag="VGE92_10925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEY1314384.1"
                     /translation="MKLRLKLAAAMLAAVTAGPALFIAAPVRAAVPAPVDAAPMPSLA
                     PMVKRVSPAVVNIATRGTIKEEPGQRNPLLDDPFFRRFFDAPPDSKPRERQFQSAGSG
                     VIVDAKNGYIITNHHVVENASEITITLLDNRTFSAKVVGSDEGADIAVLQVKQPNLIA
                     MALGDSTKLEVGDYVVAIGNPFGLQHTVTAGIVSALGRTGINPDGYEDFIQTDASINP
                     GNSGGALVNLRGELIGINSAILSRSGGNIGIGFAIPVNMAKGVMDQLIKYGQVKRGVL
                     GVNIYGVTPDIAKEFGLTESSGALVAGVAPGSAAERAGVKTGDIITSINGVTMRDAGE
                     LRNTIGMLRVGDKVEIGLLRDGKPRQVTALIAERSDLESANALDIHKGLDGAELADAA
                     AGGGVLVKTVQEGSPAAQNGLRPNDLIVGVGRTPIANTKAFKEAAKNASVLVLNVRRG
                     SIAQLIPIR"
     gene            complement(7645..8076)
                     /locus_tag="VGE92_10930"
     CDS             complement(7645..8076)
                     /locus_tag="VGE92_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY1314385.1"
                     /translation="MTVVRYEPWALVSRLQKDIDRLFGAPLTTAADSGAWLPPVDIHE
                     EANQFALHVDLPGVDPKAVEITADQGVLTIRGQREDAKRETREGYRRVERITGEFQRR
                     FSLPDSADTTNIKAKFVNGVLEVAIPKLAQVQPHRITVEAA"
     gene            8582..12049
                     /locus_tag="VGE92_10935"
     CDS             8582..12049
                     /locus_tag="VGE92_10935"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEY1314386.1"
                     /translation="MNTVVKIQPKEVGDISFQEASFDIWDKKYRLTAKDGSPIDNSMD
                     DTYKRVARALADVENEEIREEWYESFLWALRHGAIPAGRVTSNAGALEHKPATSTINC
                     TVSGTIRDSMDDILNKVHEAGLTLKAGCVAPGTWVRTEQGLVTADDAVAQRHREILCY
                     DRDERRFEMQPIRRHLTVHVPQEENVRITSNLASLTTSCKHPVLVYRSGEYRYVRADE
                     VGLTDALVHHHFSWAGDPERAREAWFAGAHLGDGSAYEKKFSYKPTRLAWARRAGTLG
                     RRLVFKIRAAEREVVERYAEFFNRLAGSQARVTVATTSYGTTVWDYAVSSFAASRAEA
                     LIDGQIGDKCATLRVPQWIASQPERHFLPFIAGLIDTDGTVSTDSGSAIISMQSTGFA
                     AELQSLLALFGVHAGITVRKSREHVLSGSLVRDCGGAMLKICDSQFLAATAQFMVDGR
                     KRERIFQYASVSGQYDEFQMLPALRDALERETQSLSHTDKQQLGFYHGYPRRDRISRI
                     WLDRWAARFPFLKDLIELTKQLRPVSRVERAVPMAETFFDFTVEKHNNYLAGNNGLVV
                     IHNCGIGYEFSTLRPRGAYVSGAGAYTSGPLSFMDIYDKMCFTVSSAGGRRGAQMGTF
                     DIGHPDVLEFIRAKRENGRLRQFNLSLLITDEFMKAVKSDGEWQLAFPLARLEYEAER
                     PDLKDSARFVWREWPYTEGYVANEAGLVACKVYKTLPARRVWDLIMSSTYDFAEPGFI
                     LVDEVNEMNNNWWTENIRATNPCGEQPLPPYGSCLLGSVNLTKFVVDPFTDNARFDWE
                     TYRKVVKVFTRMLDNVVEINGLPLAPQRDEITRKRRHGMGFLGLGSSITMLCMKYGSK
                     KSVAFSQNVSREMAVAGWEAALELSREKGPAPIMNEEFTVTPAMLRKRPEMVKDGWRV
                     GARVAGRVLHARYSRYMQRIAEAAPELVDELAEVGARFTHHTSIAPTGTISLSLANNA
                     SNGIEPSFAHHYFRNVIREGKKSKEKIDVFSFELLAYRELVNSRAMPDSQNPAEKLPD
                     YFISADDIGPKEHVDIQAAAQMWVDSSISKTANVPTDYKYEDFKDIYMYAYDQGLKGC
                     TTFRFNPEAFQGVLVKGDDLKNTTYVFTLEDGSEVEARGDQEVEYDGEMHTAANLFDA
                     LKEGYYGKF"
     gene            12052..12756
                     /locus_tag="VGE92_10940"
     CDS             12052..12756
                     /locus_tag="VGE92_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314387.1"
                     /translation="MAHIKIDRKITKYRVQKPEAESEAPAAATEAPKAPDNVIWMHEK
                     LERPEVLIGSTYKIKTPVSDHAMYVTINDILLNEGTPHEQRRPFEVFINSKNLDHFQW
                     IVALTRVISAVFRKGGDTTFLVDELKAVFDPKGGYWQPGGKFMPSTIAELGYVIERHL
                     QSIGLLKKPELDVHQRKLVEEKRAEFEARTKQTDAFSKSHFPEGAQLCAKCSTAAVVM
                     MDGCNTCLNCGDSKCG"
     gene            complement(12851..13591)
                     /locus_tag="VGE92_10945"
     CDS             complement(12851..13591)
                     /locus_tag="VGE92_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY1314388.1"
                     /translation="MSADELRTPTPKQNHLLAALPSEIQSRLFPLLELVSLSLGQVLY
                     ESGDAMHYVYFPTDSIVSLLYVMENGAAAEISVVGNEGLIGVAVLMGGESTPNQGVVQ
                     IAGSAYRLKRQLFKNEFDRGGEMQILLLRYTQSMLTQMAQTAVCNRYHSLDQQLCRWL
                     LLCLDRLPGNELTMTQDFIATMLGVRREGVTASAGKLQKLGAIKYGRGLITVLDRAML
                     EKLSCECYLVVKRETERLLPYPRRFVPP"
     gene            13737..14009
                     /locus_tag="VGE92_10950"
     CDS             13737..14009
                     /locus_tag="VGE92_10950"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY1314389.1"
                     /translation="MRLRRIRSDWRTRIAADAVAFAEKILHALRLPNNIDHHSLLATA
                     SIGIGLYPHDGVDPQILLHIADDAMYRAKRNGGNACQLSSRSVEYQ"
     gene            14149..>15462
                     /locus_tag="VGE92_10955"
     CDS             14149..>15462
                     /locus_tag="VGE92_10955"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY1314390.1"
                     /translation="MQLLGLLLAPLLHCPTAGAETPDPFAHPRELEQDVRFWIRVYTE
                     VTTDQGLLHDDWNLGLVYEVLRFDPAASPAQRERRVQDAKAHYAAMLRQFAAGETDNL
                     TPHELRILHAFGEKATPRDFRDAIERIRFQLGQADRFHEGLIRAAVWEKQIAKTLTQH
                     GVPAEIAALPHVESSFNFAAYSKVGAAGLWQFMPSTAKRFMRVDSIVDERLDPYSATE
                     AAANLMLYNYRLLGTWPLTVTAYNHGPGGLRRAQEELGTSDIVVIVKRYQGATFGFAS
                     RNFYVAFLAALEVDRNAERYFGPITRLPDTDSTPVELPDYIPVDALAKAFKVDLGALR
                     VLNPALRPPIWNGSRFVPRGYALRLPGTPPQAEIAAAWARLEPAQRYVAQRNDGSHKM
                     RRGETLAGIAAARITGAAAPSARDAGSGMRTLSPRLTLRFSPQPLA"
BASE COUNT         3020 a         4776 c         4768 g         2898 t
ORIGIN      
        1 accagcaccc gtcccgagcc gttttgtgcg atgtagtagt tgccgtcggg gcccaatttg
       61 aaaccgtccg ggccgttgta ggcatccgcg cccgcagtcg gcggggcgag atcctgcagc
      121 cgcgcccaca ccgtgcgcgg accgagcgtg ccgccgggca acacctggaa cgataggatg
      181 cggcccgcca gcatttccgc cacgagcaga tagcgaccgt ctttggacag cgtcaggccg
      241 ttcgcatagt gaatgaggtt agccacctca agcggggcgc cgccgtgcgc cgccaggtgc
      301 agaagcgtgc cactgatcgg cgcctttagg tcatagacgc cggacgcgga tacgtagaca
      361 ccgccgctgc cgtccgcggt gaaatcgttc ggcccgatga acggcttacc cgctgcgtct
      421 ttatcgatgc tgcgaatctg cttgccgttg gcgtccaact cgacgatttc attggcgtcg
      481 taacacgcga ccagcagatg atcgcgtccg aacacgatca gacccgaggc accgcagtgc
      541 tcgccgttcc agtaggtggc gacgcgcttt ccgtcccagc ttttgatgcc gggccctgcg
      601 tattcgacgt acagcagccg gccgtctttc cacagcggcc cttccggata gacggccgcg
      661 tcatccacca gcgtcgtggc ggcgcgcgcg cccaagagcg gcgccgccgc caaaagaacc
      721 gcacccagcg cccgcatcca agcggctttc attgccgcac tcccgagtgt tcgcgcaatt
      781 ccgcgatcac ctgcgcgcgc cgctggtgca gacgctcggc tatcttggcg ccgctatgcg
      841 ccgcgatgtc ttcggccgag ggtttgatcg cggctgcggc atcgcgcgcc gcctgcaggt
      901 acgcgcgctg cggatagggc acgtgctcga gtccggtgcg gccgcgcgca tccgcctcgc
      961 aggcgagcag ggctagggcg aagcgttccg gccgccggaa tgcgtcggcg cgctcgatga
     1021 attccaagat ggtgttgggg cgcagttgaa aagcacgatg cacgttgccg tgatagcgcg
     1081 ccacgatgat gccgaggtca cgatattccg cgggcaggcg caggcgatcc gccaacgctt
     1141 cgatcagtga aacgctgcgc tcttcatgac cgcgatggcg cggccactcc gtgcgcggcg
     1201 tcgtgccctt gccgagatcg tgaaccagcg cggcgaaccg aacgcgggag tcgcgactca
     1261 gcaaggcggc ttggtcgagc accatcaggg tgtgtatgcc ggtgtcgact tccggatgcc
     1321 actgcaccgg ctgcggcacg ccgaagagcg catccaattc cggataaatc ggccgcaacg
     1381 catcgcaggc gcgcaacacc ttgaagaact cgctcgccgt gggttcgcgc agcgccttct
     1441 cggtctcctg ccagacccgt tcgggcacca acgcgtcgac ttcgcggcgc tccaccatgg
     1501 cgcgcatcag ctcgagtgtt tcgtcggcga catgaaaacc gagcggcgcg aagcgtgccg
     1561 caaagcgcgc cacgcgcagc acccgcaccg gatcctcgat gaaagccggc gacacgtgcc
     1621 gcaggacccg cgcgtcgaga tcgcgcttac cgccgtgcgg atcgagcagc gtgccgtcgg
     1681 ttgcctgcgc catggcattg atcgtcaggt cgcgtcgcgc caagtcttct tcgaggctga
     1741 cctcgggtcc gaactcgacc gagaatccgc ggtagccggg cgccaccttg cgctcgaggc
     1801 gcgccagcgc gtactcctca tgagtgtcgg ggtgcaggaa aacagggaag tcgcgcccaa
     1861 cctcgcgaaa cttcaggcgc agcaactcct cgcgcgtgct gccgacgacg acccagtctc
     1921 gctccttgac ctcgagacca agcaaggcgt cacgaaccgc tccgccaacc agatacactt
     1981 gcatgcgtga atcttagcag catcaacgcg acaatccgcg cggcctttgg cctcgccgtg
     2041 attgcgagtt gcagcggcgg ctgctacctg ctgcaaagtg cgcagggcca gcttgccctc
     2101 atgtcccaac gcgaaccgat aacccgcatc atcgatcggg ggtcgacgcc gccggcgttg
     2161 cgcgcccagc tcgaacaagt cgccgccatc cgcgactacg cgagccatgc gctgggcttg
     2221 ccggacaacg gcagctatcg aagttacgcg gatgtcgggc gccgctatgt cgtatggaat
     2281 gtggtggccg cagccgaatt ttcggtcgat gcgaaggatt ggtgttaccc gatcgtcggt
     2341 tgcgtggcct accgcggtta cttcgtcgag cagcgcgcca ggcgctacgc ggccggattg
     2401 cgcgccgcgg gcttcgacgt cgcggtcggc ggagtggctg cgtattcgac gctcggccat
     2461 ttcaacgatc cggtgctcaa taccatgatt ggctggaacg atgtcgagct tgcggcgatc
     2521 atctttcacg aactcaccca ccagctgtta tatgtcgcca acgattcatc gttcaacgaa
     2581 gccctcgcga ccacggtcga agaggagggc gtgcgccgct ggctgcgcag ccaaggccgg
     2641 gacgcggatc tggcgaacca cctggcggag cacgaacact atgtcaaggt ggtcgagctg
     2701 ctcggcgcta cccgcgaaga acttcgcgcg ctgtatgctt cggcgctcgc gccgcagatg
     2761 cttcgcaaca gaaaacgcgc cgcgttcgcc gcgttgcgtt cgtcgtatgc ggttctgaag
     2821 gcgagctggg gcggacatgc gccgttcgat gcatggttcg acgaggacct caacaacgcg
     2881 cacctcgcat cgatcgcgac gtacttcaac tgcgtgccgg ggttcgagcg cgagctcgcg
     2941 gcgcgcggcg gggatttgac cgccttttac gcccgggccc gcgagctcgc aagattcgat
     3001 caggagaaac gcgatgccat cgtgtgtgcc gctccataga ccttcagatt accaagctga
     3061 gttgaatcac gagcccgatc gcggagacta tggtgacggc gagcaatccc tgagcgaatc
     3121 gggtggccaa gttgattggg ttcatgatgt actccaaagc gtaagttgat ctcgacgtgg
     3181 tacagagtgc tgcatgcgcc ccgtggcaac aaacgcaatg tctgatactc gattaaggaa
     3241 aacttaaggt gcccgctgcc tacgatcggc tgcccttggg agcgttgcgc gtgttcgagg
     3301 cggtcgccac gcacttgaat ttctcgctcg ccgccgatgc actcagcgtc acgcccgcgg
     3361 ccgtcagcca gcagatcaag gcactggagg gctacatcca agtgccgctg tttcgccgca
     3421 cgggacggcg cgttgaaatc accgaggaag ggctcgagtt gttgccggcg gtcagagcgg
     3481 gtctcgagaa actcgagtcc gccctgcagc aaatcaagct gcaccgccgg gcgggaccct
     3541 tgcaaatcac cacgctcgct tcgtttctac agatttggct gttgccgcgc attcgctcgt
     3601 ttcggcgcaa gtacccgagc gtcgagctgc gtttccacac ctcgcgcgag ctggtggatt
     3661 tctcgcgcac caccaaccac atcgccattc gctttggaca cggcgactac ccgagtctgc
     3721 actgcgaaaa actcctcgac gattggctgg tgccggttgc aagccccgat ctcatcaagc
     3781 aatacggaac gatcgagcgc ggagcgaagc tcgcgaagta tccgctgctc gagagcggcg
     3841 atgagccgtg gagcgtctgg agccagaccg gtgaggaggc cgcctggctg tcgcgggcgc
     3901 cgagcatcga cgactccgcc ggcctgctga cggcggcgga ggaagggctt ggatacgtcc
     3961 tcgcgcgatg gactctcgtt acccgcgcgc tgcacaaggg cacgttgcgc ctggcaggca
     4021 aaggggcgtt accttacgga tctgcgtatt acttcgtgtg tccaaaaaat tatttgaccc
     4081 tgcccaaggt cgcgcagttt cgcgagtgga tcatcgccgc ggcgcgcgat ttcccgcggc
     4141 cggcgacggc cgactcgaac tagagtgcga gcagagcagt cgcgagcgca tcgacgccgc
     4201 tgcgcaccgg gtcgcacacc ggcagtccga gttcggcagt caggcgctcg aagtaggcgc
     4261 tgcagtcggc ggcggacagg cccgaggagt tgatgctcaa gccgatgcag cggacagcgc
     4321 ggttcgtgag ccgcgcggcg cgcagatact cgtcgatcgc cactcgcaga tcgggaagcg
     4381 ggaactccgg gtactcgtcg atggtcaggc gcgacgggtc gtggcacaac acgagggcgt
     4441 cgggctgcga accatgcagc aggcccaaag tgacgcccgc ataggcggga tgaaatagcg
     4501 atccctggcc ctcgatgacg tcccagtgct cgggggcatt gtcgggagac agctgttctg
     4561 cggcgccggc gatgaagtcg gcgatgaccg catcgatcgc gataccctga cccgaaatca
     4621 tgatgccggt ttggcccgtc gcccgaaacg tggccgccac cccgcgccgc gacaagttcg
     4681 cggcgagcgc gagagccgtg tacttcttgc cctgggcgca atccgtgccg accgtcagaa
     4741 cccgcttgcc gctgcgcttg cggccgctcg cggcgccgaa ctgctgctcg ctgtgccgca
     4801 catcgatgag ccgcacgccg cgccgcgccg cgctctgcac aagttccgga aaagaggcga
     4861 gacgtgtatg caggccgctc accacgtcaa gtccggcgtc agccgccgcc tcgagttcgg
     4921 cgacccaatg acgcggcagc gcaccgccgg tcggcgcgat gccgacgacg atcgagccgg
     4981 cgccgcgcgc ggccgcctcg cggggcgaca ggcgcggcag cccgagggtc acggcgcagc
     5041 cgggcaagct ccattcgccg atcacatctt gcgcgcacca atctttgagg ccgaaggcgg
     5101 ttttcgcgct cagcttatct tgcacgtcgc cgagaaacag caagtaaggt ttgcgcaagc
     5161 gagcggcact acgagcgcag cggccggttg cggccggcac tcgcgcggtt gtggctgagg
     5221 cgcgcattgt cctcgtaggc gcccaagtat tgccgcgccg aggccagcat cgattggggc
     5281 ttcaaaccga gtttcgcgaa accgtcttgg gtgcagacgc tgtcgatttt caacgaccga
     5341 taattgtcgc tggagaaagg ccgacccggc acgaaatcca tgacgagcgc ctgagtccgc
     5401 gccacgacgt tgggtaaacc gatgatccag cggcgtcggc cggtcagctt gcccaccaaa
     5461 gcgacaatct cacgcaatga ataaacctgc gggccgccga gttcgcaggt ttgccggctg
     5521 ctggcgccgc catgcagcga gcgcagcagc gcttcgatca catcgtcgac gaacacgggt
     5581 tgaaagcggg tgttcggcct cgccaacgga aagatgaaag gaatcgaggc gagcaatgcg
     5641 gcgaagcgat tgagaaaaga atcgccggga ccgaacatca ccgacggctg gaagatggtc
     5701 caatcgagcg cgaaattggc atcgcgaatc agctgctccg cctcgccctt ggtgcgcagg
     5761 taatagctcg gcgcgtcgac ggcggccttc aatgcagata cttgcaacag ccgggcgaca
     5821 ccggccgaac ggaccgccag caatactccg cgggtgagtt caacgtgcgc acgccggaag
     5881 cctccgccgc cgaagccgcg ctcgttgagt atgccgatga gattgatcac gacgtctttg
     5941 ccgcgaaacc gctcgctcaa ctgggcttcc tggtacacat cgcagttctc cagcgtgagt
     6001 ccgggcaata ccagaagatg cttgtgctgt tcccgatccc gcgacaacac ggtgacgcga
     6061 tggccgtcct tgatcaagcg tgcgaccagc cgggttccca ggaatccggt gccgccgaga
     6121 acagcgatgc aaagcggacg cacgattttc agcgaatcgg aatcaattgt gcgatcgaac
     6181 cgcgccgcac gttgaggacc aagacactgg cattcttcgc cgcttccttg aatgctttgg
     6241 tattggcgat cggcgtgcgg ccgacaccca cgatcaagtc gttcggccgc aacccgttct
     6301 gcgcggccgg gctcccttct tgtacggtct tcaccaaaac accgccgcca gcggcggcgt
     6361 cagccagttc cgcgccgtcc aagcccttat ggatatcgag cgcattggcc gactcgaggt
     6421 cgctgcgctc ggcgatcagc gccgtcactt ggcgcggctt gccgtcgcgc agcagaccga
     6481 tctcgacctt gtcgccgact ctcaacatgc cgatggtatt gcgcaattcc cccgcgtctc
     6541 gcatggtgac gccattgatg gaggtgatga tgtcgccggt cttgacgccg gcgcgctcgg
     6601 ccgccgaccc tggcgcgacg ccggccacca acgcaccgct cgattcggtc agtccgaatt
     6661 cctttgcgat gtcgggggtc acgccgtaaa tgttgacgcc gagaacgccg cgtttgacct
     6721 ggccgtattt gatcaattgg tccatgacgc ccttggccat gttcaccgga atcgcgaaac
     6781 cgataccgat atttccgccg ctgcgcgaca gaatggcgga attgatgccg atcaattcgc
     6841 cgcgcaaatt caccaacgcg ccgccggagt tacccgggtt gatcgaggcg tcggtctgta
     6901 tgaagtcttc gtagccgtcg ggattgatgc cggtgcggcc gagtgcactg acgatgccgg
     6961 cggtcaccgt atgttgcaat ccgaacgggt tgccgatggc gacgacgtag tcgccgactt
     7021 ccaatttggt cgaatccccc aaggccattg cgatcaggtt gggttgcttg acttgcagca
     7081 ccgcgatatc cgccccttca tcggagccga cgaccttggc cgaaaaggtg cgattgtcga
     7141 gcaaggtgat ggtgatttcg ctggcgttct ccaccacgtg atggttggtg atgatgtagc
     7201 cgttcttggc gtcgacgatg acgcccgagc ccgcgctctg gaactgtcgc tcgcgcggct
     7261 tcgaatccgg cggcgcgtcg aagaagcggc ggaaaaacgg gtcgtcgagc agcggattgc
     7321 gttgaccggg ctcctctttg atggtgccgc gcgtggcaat gttgacgacc gcgggcgaga
     7381 cgcgcttgac catgggggcg agcgacggca tgggcgccgc atccacgggc gcgggcacgg
     7441 ccgcgcgcac gggtgctgca atgaagagcg ccggtccggc ggtgacagcg gcgagcatcg
     7501 ccgccgcaag tttgagacgc agtttcatgc gcggtccttt ccgtctgatg ttcccggcag
     7561 gcgaatgagg ccggacccta cggtccggcc tgcagctcgt ccgcttcgcg atattacacg
     7621 gaagattgcg cttggcgggc tctcttaggc cgcttccacc gtgatccggt gcggttgcac
     7681 ttgcgcgagt ttcggaattg cgacttccag cacaccattg acgaacttcg ccttgatgtt
     7741 ggtggtgtcg gccgaatccg gcaagctgaa acggcgctgg aactctccgg tgatgcgctc
     7801 gacacggcga tagccctcgc gggtttcgcg cttggcatct tcacgctgcc cgcgaatcgt
     7861 caacacgccc tggtcggcag tgatttccac cgctttagga tcgacaccgg gcagatccac
     7921 gtgcaatgca aactgattcg cttcctcgtg gatgtcaacc ggcggcaacc aggcgccgga
     7981 atccgcggcc gtggtcaacg gcgcgccgaa taaacgatcg atatctttct gtagccggct
     8041 gacgagtgcc cacggctcat aacgtacgac ggtcattgct aatactccta aataaaaaat
     8101 tgtttgttca gatgctgctg ttctctcgat tcgaaatcac gcgcgtgaca gcgaaattgg
     8161 gccgcgcttg cgcatttcaa cggagccgcg caaaaggcgc tgcgcggagc gcgaaatgag
     8221 acgtggttca aagtcaatgg aacataatgt ggataacgtg tggaaacaca agatgttgcg
     8281 attcggcgct tttgcgcctg ccgcggcaac ttgcaaatat aatcagctaa tttctcggga
     8341 gatttttgta agatgttgat aaacatgtgt ttttcaagcc aaaaaaacat tgaaaaatct
     8401 cttgacgatg gttgccatca cgacttaccc tctccctccc cacacaagat gttgtggtcg
     8461 gcggctcgca gtcccccatc ggaattggtt cggtaaccag cgtttttatt tttcgcgaga
     8521 ccgctgcaac cgttcggact ttttttgttt gagagttaat tagggagtgg ctttaatacc
     8581 aatgaatacg gtggtcaaaa tacagcccaa agaagtaggc gatatctcgt tccaggaagc
     8641 gtcgttcgac atctgggaca agaaatatcg actgaccgcg aaagatggat cgcccatcga
     8701 caattcgatg gacgatacct acaagcgcgt tgcgcgtgcg ctcgccgacg tcgaaaacga
     8761 agaaatccgc gaagaatggt atgagtcgtt cttatgggcg ttgcgccatg gcgcgattcc
     8821 cgccggccgc gtgacctcga acgccggcgc gctcgagcac aagccggcga cgtcgaccat
     8881 caattgcacg gtttccggga ccatccgcga ttcgatggac gatatcctga acaaggttca
     8941 tgaggccggc ctcacgttga aggcgggctg cgttgcaccg ggtacttggg tgcgcacgga
     9001 gcaaggtctc gtgaccgcgg acgacgcggt tgcccaacgg catcgcgaga tactgtgcta
     9061 cgaccgcgac gagcgtcgtt ttgagatgca gccgattcgg cggcacttga cggtacacgt
     9121 gccgcaggag gagaacgtcc gtatcacctc gaaccttgca tcgctcacga cctcctgcaa
     9181 acacccagtg ctcgtctatc gcagcggtga atatcggtac gtgcgcgccg acgaggtagg
     9241 gcttaccgac gctctcgttc accatcattt ctcgtgggcg ggcgatcccg agcgagcgcg
     9301 cgaggcgtgg ttcgcgggtg cccatttggg cgatggctcg gcgtatgaga aaaagttcag
     9361 ctacaagccg acccggttgg cgtgggcccg gcgcgccggc actctcgggc gccgtctcgt
     9421 cttcaagatc cgcgccgcag agcgcgaggt cgtggagcgc tatgctgagt tcttcaaccg
     9481 cctggccggg tcgcaggcca gggtaacggt cgccacgacg tcctacggaa ccactgtctg
     9541 ggactatgcc gtgagcagct tcgcggcaag ccgcgctgaa gccttgatcg acggccaaat
     9601 cggcgacaag tgcgcaacgc tgcgcgtacc gcaatggatc gcttcccaac ccgagcgcca
     9661 ctttctgccg tttatcgctg gcttgatcga cacggatggc accgtttcca ccgactccgg
     9721 cagcgcgatc atcagcatgc agagcacggg cttcgcagcc gagttgcagt cgctcctggc
     9781 gctattcggc gttcatgcag gcatcaccgt tcgcaagagc cgcgaacacg tgctttcagg
     9841 ctcgctggtg cgcgactgcg gcggcgccat gctcaagatc tgcgactcgc agtttctagc
     9901 ggccaccgcg cagttcatgg tcgacgggcg caagcgcgag cgcatcttcc agtatgcgtc
     9961 ggtcagtggg cagtacgatg aatttcagat gctgcccgcg ctgcgcgacg cgctcgagcg
    10021 agagacgcaa tcgctgtcgc ataccgataa gcagcaattg ggcttctacc atggctatcc
    10081 gcgacgcgac cgcataagcc gaatatggct ggatcgatgg gccgcacggt ttccgttcct
    10141 gaaagacctc atcgagctga cgaagcagtt gcgtcccgtc agtcgtgtcg agcgcgcagt
    10201 gccgatggca gaaacttttt tcgacttcac cgtcgagaaa cacaacaatt accttgccgg
    10261 caacaatggt ctggtagtca tccacaactg cggcatcggc tacgagttct ccaccttgcg
    10321 gccgcgcggc gcgtacgtat cgggtgcggg cgcatatacc tcgggcccgc tgtcgttcat
    10381 ggatatctac gacaagatgt gtttcaccgt gtcctcggcc ggcggccggc gcggcgcgca
    10441 gatgggtacg ttcgatatcg gccatcccga cgtgttggag ttcattcgcg ccaagcgcga
    10501 aaacggacgc ctgcggcagt tcaatctgtc gttgctgatc accgatgagt tcatgaaggc
    10561 cgtgaagagc gacggcgagt ggcaactggc attcccgctg gcgcgtctgg aatacgaggc
    10621 ggagcgcccg gacctgaagg attccgcgcg tttcgtgtgg cgcgaatggc cgtataccga
    10681 gggctacgtt gcgaacgagg ccggcttggt cgcctgcaag gtctacaaga cattgccggc
    10741 gcgtcgcgtg tgggatctga tcatgtcctc gacgtacgac ttcgccgagc cggggttcat
    10801 tctcgtcgat gaagtcaacg agatgaacaa caattggtgg acggagaaca tccgcgcgac
    10861 gaatccgtgc ggcgagcagc cattgccgcc gtatggctcc tgcctcctgg gatcggtcaa
    10921 tctcaccaag ttcgtggtcg acccgttcac cgacaacgct cgcttcgatt gggaaaccta
    10981 ccgcaaagtg gtgaaggtgt ttacgcgcat gctcgacaat gtggtcgaga tcaacggctt
    11041 gccgctggcg ccgcaacgcg atgagatcac ccgcaaacgc cgccatggca tgggcttttt
    11101 ggggctgggc tcgagcatca ccatgttgtg catgaagtat ggctcgaaga aatccgtcgc
    11161 cttttcccag aacgtgtcgc gggaaatggc cgtggcggga tgggaagccg cgctcgagtt
    11221 gtcgcgcgag aaaggaccgg cgccgatcat gaacgaggag ttcaccgtca cccccgccat
    11281 gctgcgcaag cgccccgaga tggtcaagga cggctggcgc gtcggcgcac gcgttgcggg
    11341 cagagtgctg cacgcgagat atagccgtta catgcaacgc atcgccgaag cggcgcccga
    11401 gctggtggac gaactcgccg aagtcggcgc gcgtttcacc catcacactt ctatcgctcc
    11461 gacgggaacg atttcgctgt cgctcgccaa caacgcgtcc aacggcatcg agccctcgtt
    11521 cgcgcatcat tatttccgca atgtgatccg cgaaggcaag aagtccaagg agaagatcga
    11581 cgtattctcg ttcgagttgt tggcatatcg cgaactggtg aattcccgcg ccatgccgga
    11641 ctcgcagaat ccggcggaga aactgcccga ctatttcatc tccgcggacg acatcggccc
    11701 gaaggaacac gtcgacatcc aggcggcggc gcagatgtgg gtggactcct cgatttccaa
    11761 aacggcgaat gtcccgaccg actacaagta cgaggatttc aaagatatct atatgtatgc
    11821 ctacgaccag ggcctgaagg gctgcaccac cttccgtttc aatccggaag cattccaggg
    11881 tgtgctcgtc aaaggcgacg acctcaagaa caccacctac gtgttcactc tcgaggacgg
    11941 ctcggaagtc gaggcgcggg gcgatcagga ggtcgaatac gacggtgaaa tgcacaccgc
    12001 cgcgaatctc ttcgatgcac tgaaagaagg ctattacggg aaattctgag catggcgcac
    12061 atcaaaatcg acaggaagat caccaagtac cgggtgcaga agccggaagc cgagagtgaa
    12121 gcgccggccg ccgccacgga agcgccgaag gcgccagaca acgtgatttg gatgcacgag
    12181 aagctcgagc gccccgaggt gctgatcggc tcgacgtaca agatcaagac gccggtgtcc
    12241 gatcacgcga tgtatgtcac gatcaacgac attctgctca acgaaggcac gccgcatgag
    12301 cagcgccgcc cgttcgaggt attcatcaac tcgaagaatc tcgatcactt tcagtggatc
    12361 gtggcgctga cgcgggtcat ttccgcggtg tttcgcaagg gcggcgacac caccttcctg
    12421 gtggatgagt tgaaagcggt tttcgatccg aaaggcggtt attggcaacc cggcggcaag
    12481 ttcatgccct cgaccattgc cgaattgggc tatgtcatcg agcgtcacct gcagtcgatc
    12541 ggactgctca agaagcccga actcgacgtg caccagcgca aactcgtcga agagaagcgc
    12601 gccgaattcg aggcgcgcac caagcagacc gacgcctttt caaagtcgca tttcccggaa
    12661 ggcgcgcagc tgtgcgcgaa atgcagcacc gccgccgtgg tgatgatgga cggctgcaat
    12721 acgtgcttga attgcggcga ttcgaagtgc gggtaattcg ccgataatca agccaatcac
    12781 aatgggggcc tcataagctg caacttggat cccaaaagag cagccgcggc acccgtgagt
    12841 gtggcgcgcc ttatggcggt acaaaccggc ggggataggg aagcagccgt tctgtttccc
    12901 tcttgaccac gaggtaacac tcgcaactca gcttctcaag catcgcgcgg tccagcaccg
    12961 tgattagacc gcggccatac ttgattgctc ccagcttctg gagctttccg gcggaagccg
    13021 tcaccccttc gcggcgcaca ccgagcatgg tggcgataaa gtcctgggtc atcgtcaatt
    13081 cgttgcccgg caagcgatcg aggcacagaa ggagccagcg gcacagctgc tggtccagcg
    13141 agtgataccg gttacacacc gctgtctgcg ccatttgtgt gagcatcgat tgcgtgtagc
    13201 gcagcagaag aatctgcatt tcgccgcctc gatcgaactc gttcttgaat aactgtcttt
    13261 tcaaccgata ggcggatccg gcgatttgca cgacaccctg gttcggcgtg ctttcgccgc
    13321 ccatcagcac ggccacgccg atcagacctt cgttgccaac gacggagatt tccgccgccg
    13381 ccccgttttc catgacatag agcaatgaca cgatggagtc ggtcggaaaa taaacgtaat
    13441 gcatcgcatc gccggactca tagaggacct gacctaggga caacgatacc aattcgagaa
    13501 gagggaacag acggctctga atttcagatg gcaaagcagc caatagatgg ttctgcttcg
    13561 gagtaggagt cctcagttca tcagcactca tcagagtagt cataagcacc tgtacgtcca
    13621 tcacggcatt gtttggggga tacaccatca acgccacacc agatatcaca gcaactaagc
    13681 ggcctggtct atacggaatt gccgcaccca ggcagttgtc atgagcacgt ttttgtttgc
    13741 gcctgcggcg cataaggagc gattggcgaa ctcgcatcgc ggccgatgcc gtcgcatttg
    13801 ccgagaagat cctgcacgcc ctgcggcttc cgaataacat cgatcaccat agtttgctgg
    13861 ccaccgcaag tatcgggatc gggctctacc cccacgacgg cgtcgatccg caaatcttgc
    13921 tgcacatcgc ggacgatgca atgtaccgtg caaaacgcaa tggcggaaac gcctgccaac
    13981 tttcgagtcg atcagtggaa tatcagtgat cgcagttggc cctatgcttt cggacatagc
    14041 ctatacttgg acttagagct gcgcggcgct caaaggcgct ggggtctttt ggccgcagct
    14101 tatggtgttc tgcccatttg taagacattg ttttcgcgta cttcatggtt gcagcttttg
    14161 gggctgttgc tcgcgccgct gttgcactgc ccgactgccg gcgccgaaac tcccgaccca
    14221 ttcgcccacc cgcgagagct cgagcaggac gtgcgtttct ggattcgggt ctataccgaa
    14281 gtcaccaccg accaaggatt gctgcacgac gattggaatt tagggctggt ctacgaagtc
    14341 ctgcgtttcg atcccgccgc ctcgccggcg cagcgcgagc ggcgcgtcca ggacgcgaag
    14401 gcgcattatg cggcaatgct gcggcaattt gccgccggcg agaccgacaa tctcacgccg
    14461 cacgagctgc gaattctgca tgcgttcggc gaaaaggcga ctcccaggga ctttcgcgat
    14521 gcgatcgagc gcatacgctt ccaattggga caagccgacc gatttcacga agggttgatt
    14581 cgagccgccg tgtgggaaaa gcagattgcg aagactctca cgcagcatgg cgtacccgcg
    14641 gagatcgcgg cgctgccgca tgtcgaatct tcgttcaact tcgccgccta ttcgaaagtc
    14701 ggcgcggccg gactgtggca attcatgccg tcgaccgcca aacgattcat gcgagtcgac
    14761 agcatcgtcg acgaacgcct ggatccctac agcgccaccg aagcggcggc caacctgatg
    14821 ctgtacaact accggttgct cggcacctgg ccgttgaccg tcaccgccta caaccacggt
    14881 ccgggcggcc tgcggcgcgc gcaggaggaa ctcggcacca gcgacatcgt cgtcatcgtc
    14941 aagcgctacc agggcgcgac cttcggcttc gcatcgcgca atttctacgt cgcgttcctg
    15001 gcggctctcg aagtcgaccg caacgccgag agatatttcg gaccgatcac ccgtctgccg
    15061 gacaccgact cgacgccggt ggaactgccg gattacatcc cggtcgatgc gctggcgaag
    15121 gcgttcaagg tcgacctggg cgcgctgcgc gtgctcaatc ccgcattgcg gccgcccata
    15181 tggaacggca gccggttcgt gccgcgcggt tatgcgctgc gcttgccggg tacgccgccg
    15241 caggccgaga tcgctgccgc gtgggctcgc ttggagccgg cgcagcgcta tgtcgcgcag
    15301 cgcaatgacg gcagccataa gatgcggcgc ggcgagacct tggcgggcat cgctgcggca
    15361 aggatcaccg gtgcagccgc accctccgct cgagacgcgg gcagcgggat gcgcacgctc
    15421 tcgccgcggc tgacattgcg gttttcgccc cagccgttgg cg
//
LOCUS       DASWZT010000388         2911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_95148, whole genome shotgun sequence.
ACCESSION   DASWZT010000388 DASWZT010000000
VERSION     DASWZT010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95148"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VGE92_10960"
     CDS             <1..461
                     /locus_tag="VGE92_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY1314391.1"
                     /translation="IIVRETEDEAWREADRLIAHLTDEQIAKAQAGFAVSGSIGQQRM
                     AQLHHGNRDGLRIGRNLWAGVGLVRGGAGTAFVGSPDNIARALSEYQNAGVETFILSG
                     YPHLEEAYRTAELLFPAIGKGSPIFQTVQKAVESATARAGSATVGRFSSI"
     gene            910..1914
                     /locus_tag="VGE92_10965"
     CDS             910..1914
                     /locus_tag="VGE92_10965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY1314392.1"
                     /translation="MAQARVDEPQTGTAATFHEFSAQTRISGADIGERKLRKGAADAV
                     RRFVREAGGSWPAAVSEIVDRVARSGATPLVVADGCRVLGVIELHDVVKQGIREHCEA
                     LRRMGIRTIMVTGDNPLTAATIAAEIGVDEVLAEATPERKCEFIRQCQKDGHQVAMCG
                     DGSNDAPALAQADVAVAMNSGSPMAKQAGNLVALDSDPTKFIVIVETGKRIRTTLQSL
                     TAFSLAADLAKYLAILPVVFAATYPRLNALNIAQLTNPRSAILSALIFNILVILPLLL
                     FAMRGVKARAPSTARPAQCHWWLYGLAGIIATLAGIKLLDVCITAFRLVGAGRGCYDL
                     "
     gene            1968..>2911
                     /gene="dusA"
                     /locus_tag="VGE92_10970"
     CDS             1968..>2911
                     /gene="dusA"
                     /locus_tag="VGE92_10970"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: protein motif:HMM:NF008774.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEY1314393.1"
                     /translation="MTDRQAKLISRKLSVAPMMDWTDCHCRYVHRLFAPHALLYTEMI
                     VSTALLRGDARRLLEHSPSEHPLALQLGGCDPQELAAAARIGTAAGFAEINLNVGCPS
                     DRVKNGSFGACLMLKPALVADCVKALTDATSLPVTVKCRIGIDDRDDYAFFSAFADQV
                     AAAGIAALIVHARVAILGGLSPKENREIPPLKYDYVHRLKQERPGLTVVLNGGLSQLA
                     EVRRHMAAGVDGVMLGRAAYHRPAVLAELERSLIDPEWAVPEPPEIIERVVAYAKVCA
                     RRGIRLHSITRHMHGLMAGREGARAWRRFLSEVAARPGA"
BASE COUNT          516 a          894 c          963 g          538 t
ORIGIN      
        1 acattatcgt gcgtgaaacg gaagacgagg cctggcgcga ggcggaccgc ctgatcgcgc
       61 acctcactga tgaacaaatt gccaaggccc aggccggatt tgccgtgtcc gggtccatcg
      121 gccagcagcg catggcgcaa ctgcatcatg gcaacaggga cggcttgcgg atcggcagga
      181 atctttgggc cggcgtcggt ctcgtgcgcg gcggcgccgg tacggccttc gtcggcagcc
      241 ccgataacat cgctcgcgca ttgtcggagt accagaatgc cggagtggag acgttcatcc
      301 tctccgggta cccgcacctc gaagaggcgt accgtacggc ggaattgctt ttcccagcga
      361 ttggcaaggg cagccccatt ttccagacgg tgcagaaggc cgtcgagtca gcgaccgcgc
      421 gcgcgggctc tgcgaccgtg gggcggttca gttcgatctg accaaagtcc aattcaatag
      481 ttgaatcaac cgcttaggag atcgtcaaaa ggcaacaatc agcgaaaatt aacggcgtag
      541 tatcctgctg gcccgtgaac gaagctgcgc ccctcgagaa tcgccctgcc ggccttcgcg
      601 cggcggcgct gcgatcgatg ggcgtcgcgt ttctgctcgg cagctgcagg tcgcgcgctc
      661 gatgggtgcg gcgtcggccg gcgttgtggc gctctcggcg ctgatcgcgt tgttcgtgtg
      721 cattattccc ctcgctaccc gcgcatgcgt gtttgcgatc ggcatggtca gcgtggctcg
      781 gttgatgcgc gccaacgtca tcgcgagcgc cggcggcgcg ctggaagccg cagccactat
      841 cgacgtgctg gtactcgact cgcctccgtg gccgatcaga ctcccgaggg ccattcgatc
      901 gtcgcgctcg tggcgcaggc gcgggttgat gagccccaga ccggaacggc ggcgacgttc
      961 catgagttct ccgcccagac gcgcatcagt ggtgccgaca taggagagcg caagttgcgc
     1021 aaaggcgctg cagatgcagt tcgccgcttc gtgcgggagg cagggggcag ctggcctgca
     1081 gcggtcagcg agatcgtcga ccgggtagcg cgctcgggcg ccacgccgct ggtggtcgcc
     1141 gacggttgcc gagtgcttgg cgtcatcgaa ttgcacgatg tcgtcaaaca gggcattcgc
     1201 gagcactgtg aggcgctgcg ccggatgggt atcagaacca tcatggtcac cggagacaac
     1261 ccactgaccg cagcgacgat agctgccgag atcggagtgg acgaggttct ggccgaagcg
     1321 acgccggaga ggaagtgtga gttcattcga caatgccaga aggacgggca tcaggttgcc
     1381 atgtgcgggg acgggtccaa cgatgcgccg gcgctcgcgc aggccgacgt ggcggtggcg
     1441 atgaattccg gctcgccaat ggccaagcaa gccggaaacc tggttgccct cgattcagat
     1501 cccacgaaat tcatcgtgat tgtcgagacg ggcaaacgaa ttcgaacgac gctccagtcc
     1561 ctcacggcct tcagccttgc cgccgacctg gccaagtatc tggcaattct ccctgtggta
     1621 tttgccgcga cttatcctcg gctgaacgcg ctcaacattg cgcaactcac gaacccgcgg
     1681 agcgcgattc tgtcggccct gatcttcaat atcctggtca ttttgccgct cctcctgttc
     1741 gcaatgcggg gcgtcaaggc acgggcaccc tcgacggcgc gtcccgcgca gtgccattgg
     1801 tggctgtacg gactcgccgg gatcatagcg acgttggccg gcatcaagtt gctcgacgtt
     1861 tgcattactg cattcaggct cgtaggagcg gggagaggat gctacgatct ctgaagccaa
     1921 cgtggctcaa ccgaagtgcg ttacattgag ggcgtgctcc tagaaagatg accgatagac
     1981 aagccaaatt gatatcccgc aaactatcgg ttgcaccgat gatggactgg accgattgcc
     2041 attgtcggta cgtgcaccgg ctgttcgcgc cgcacgcctt gctctacacc gagatgatcg
     2101 tctcgaccgc gctgctgcgc ggtgacgcac gccgtctgtt ggagcacagt ccatccgagc
     2161 atccgctggc gctgcaattg ggcggctgcg atccgcagga actcgcggcc gcggcgcgca
     2221 tcggcacggc ggccggtttc gcggaaatca atctcaacgt gggctgtccc agcgaccggg
     2281 tgaagaacgg ctcgttcggc gcgtgcctga tgctaaaacc agccctggta gccgactgcg
     2341 tgaaggcctt gactgacgcg acgtcgttgc cggtgaccgt aaaatgccgc atcggcatcg
     2401 acgatcggga cgactatgcg ttctttagtg ccttcgcgga tcaagtcgcc gcggccggca
     2461 tagcggcgct catcgtgcac gcccgcgtgg ccatactggg cggcctttcg cccaaggaaa
     2521 atcgcgagat tcccccgctc aagtacgact atgtacaccg gctcaagcag gagcggcccg
     2581 ggttgacggt ggtgttgaac ggcggtctgt cgcaactggc cgaggttcgg cggcacatgg
     2641 cggcgggggt cgacggcgtg atgctcggac gcgccgccta ccatcgtccg gctgtgctcg
     2701 ccgagttgga gcggtcgctg atcgatcccg agtgggcggt ccccgagcct cccgagatca
     2761 tcgagcgcgt cgtcgcctac gccaaggtgt gcgcgcggcg gggaattcgc ctgcattcga
     2821 tcacgcggca catgcatgga ctgatggcgg ggcgcgaggg ggcccgcgcc tggcgacggt
     2881 tcctatccga ggtcgccgcc cgtccgggag c
//
LOCUS       DASWZT010000389         1596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_46679, whole genome shotgun sequence.
ACCESSION   DASWZT010000389 DASWZT010000000
VERSION     DASWZT010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46679"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VGE92_10975"
     CDS             <1..712
                     /locus_tag="VGE92_10975"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RHO alpha subunit C-terminal catalytic
                     domain-containing protein"
                     /protein_id="HEY1314394.1"
                     /translation="DSWGGFVFLHLTPAEAKPLAAQLAGIPERLQRYPLGELRIGVTR
                     RYEVAANWKILCENYNECYHCAGVHPELCAVVPSFREAGGANLDWNRGIPHREGAYTF
                     THSGTTQRRAFPGLNADEQVRHKGELAYPNLFLSVACDHVAAFILQPRAADRTDIVCQ
                     FLFEPHEIAKPGFDPADAVDFWDLVNRQDWAVCEGVQRGIQSRVHACGYYAPMEDWNL
                     DIRRYVTERIEAFVADRP"
     gene            806..1495
                     /locus_tag="VGE92_10980"
     CDS             806..1495
                     /locus_tag="VGE92_10980"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY1314395.1"
                     /translation="MALKRHVPKFHRATPAVRREALVDATLSCLKRYGHAGVSVRRIS
                     AAAGVSIGLINHHFPSKSTLIAETYETLSFSLLDSIKRQAGNHSVSPRDRLTNFFKAS
                     FAPELLDPQLFNVWLVFWSMVAHSAEIRSVHTRTYGKYRAVLESMLQELVESGEAPPF
                     RLRPAAIALSAFLDGFWVELSLSPTVFKPREAIALCEDWVNALCIGGFPRLLAAPKAD
                     KTGAARKPRKS"
BASE COUNT          286 a          492 c          484 g          334 t
ORIGIN      
        1 cgatagttgg ggcgggttcg tctttttgca tttgacgccc gcggaagcca aacccctggc
       61 cgcgcagttg gcgggcattc ccgagcgctt gcagcgctat cccttgggtg agctgcggat
      121 cggcgtcacc aggcgctacg aagttgcggc gaactggaag atcctgtgcg agaactacaa
      181 tgagtgctat cactgcgccg gtgtgcaccc ggagctatgc gcggtggtgc cctcgtttcg
      241 tgaggcgggc ggcgcgaatc tggactggaa ccgcggaatt ccgcatcgcg agggagccta
      301 cacgttcacg cactcgggca cgactcagcg ccgcgcattt cctgggctca acgccgacga
      361 gcaagttcgg cataaaggcg agctcgcgta ccccaacctg tttttgagcg tcgcctgcga
      421 tcacgtcgcc gctttcatac ttcaaccgcg cgccgccgat cgcaccgaca tcgtctgcca
      481 gtttttgttt gagccgcacg aaatcgcaaa acccggcttc gatccggcgg atgcggtgga
      541 cttttgggat ttggtcaacc gccaggattg ggcggtatgc gaaggcgtgc agcgaggaat
      601 tcagtcgcgg gtccatgcct gcggttacta cgcacccatg gaagactgga atttggacat
      661 ccggcgctat gtgaccgagc gaatcgaggc cttcgtcgcc gatcggccgt aaccgcggca
      721 acgggccgtc gggaccttca atatcgcgcg cttgcaacgc gccttgcgtc cgcgctccgg
      781 tataatcctc gaaaaggact ttgaaatggc actgaagcgg catgttccga aatttcaccg
      841 tgcgacgccc gcggtgcgcc gcgaagcatt ggtcgatgcg accttaagct gcctcaagcg
      901 ctatggccat gccggcgtgt cggtgcgccg gatcagcgcc gcagccggcg tctcgatcgg
      961 cttgatcaac catcatttcc ccagcaaatc gactctcatc gccgagacct acgaaactct
     1021 ctcgttctcc ctgctcgact ccatcaagcg ccaggccggc aaccattccg tctcgccgcg
     1081 cgaccgcttg accaattttt tcaaggcttc gttcgcaccg gaactgctcg atccgcagct
     1141 tttcaatgtc tggctggtgt tttggagcat ggtggcgcat tcggccgaga ttcgttccgt
     1201 gcacaccagg acgtacggca agtaccgcgc ggtgctcgag tcgatgctgc aagaattggt
     1261 ggagtccggc gaggcgcccc ccttcagact acgcccggcc gcgatcgcgt tgtccgcgtt
     1321 cctcgacggg ttttgggttg agttgagctt gagcccgacg gtgttcaagc cgcgcgaagc
     1381 gatcgctctg tgcgaggatt gggtgaatgc cttatgcatc ggcggctttc cccggctgtt
     1441 ggccgccccc aaggccgaca agaccggggc tgcgcggaaa ccccgcaaat cctagcgtcc
     1501 gctaccggcg attgttcgcc cttgttatac ttttgttcaa caagattcga ggaggccgtg
     1561 gaaaatgcaa gcgaagctct ctgtgcgaag cgacgc
//
LOCUS       DASWZT010000390         9504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_41953, whole genome shotgun sequence.
ACCESSION   DASWZT010000390 DASWZT010000000
VERSION     DASWZT010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9504
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41953"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1259)
                     /locus_tag="VGE92_10985"
     CDS             complement(135..1259)
                     /locus_tag="VGE92_10985"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HEY1314396.1"
                     /translation="MPADRGVGISDMEDVIQENEDLKKRLTELTAEAANNESILKRTQ
                     ARELTLLRAETLAQLLHAMVDGLRESYALDAVNVVLLDPQHEVRHLLLAGGDRPEEFK
                     QVFFVDSLVGLAPQLTALHKPWLGPFVGADHHLLFPGTSNLKSAALVPLTRKDRATGA
                     LCFGSRDITRFTRHHGTDFLAHLGAVAAVCVENAVNRTRLLRAGITDFLTGWANRRYL
                     QQRLKEELARAQRRAGSIACLMIDIDRFKSINDGYGHLAGDNALKEIAHRVESQIRSM
                     DTAARFGGDELTIILPEAGAAEATTLAERIREAIAAAPFTLTAQIERTLTVSIGVAAV
                     APGRHETDLKPVADRLLADADAALYRAKALGRNRVQVGAG"
     gene            complement(1249..2352)
                     /locus_tag="VGE92_10990"
     CDS             complement(1249..2352)
                     /locus_tag="VGE92_10990"
                     /inference="COORDINATES: protein motif:HMM:NF042869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEY1314397.1"
                     /translation="MNLDELRAALTDLDGQLVDLVARRQALSEQVAAVKRATGRPTRD
                     FGREREVILRGRNAAARRGVSPDLAESLLRQLIQSSLATQEHARVAAQAHGSGKTALV
                     IGGHGKMGGWFADFLASQGFRVAIADPAGGLAGFDTIADWQTDPLDFDVIVLATPLSA
                     TTRLLEFLAQRRPRGLIFDLGSLKTPLRAGLEALSKAGCRVTSLHPMFGPDTELLSGR
                     HVIFIDLANPEALQEAQELFAPTMAERVVMGLDEHDRLIAYVLGLSHALNIAFFTALA
                     DSGEAAPRLAQLSSTTFDAQLEVASRVAAESPELYFEIQSLNEYGGESLLALQQAVER
                     LVAAVKAGDSAQFSALMNRGRAYLDVRAAARAG"
     gene            2438..3115
                     /gene="pdxH"
                     /locus_tag="VGE92_10995"
     CDS             2438..3115
                     /gene="pdxH"
                     /locus_tag="VGE92_10995"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEY1314398.1"
                     /translation="MSSSKTRTETLPDPLPANPLDVAAQWLAQARLDAAQPNPNSMVL
                     ATVDARGHPSARVVLCKEISPASGSVVFYTNYRSRKGRELAGNRRAAVVFHWDHRHRQ
                     VRIEGQIERLSDAENDAYFQTRPWQSRIGAWASEQSQPVASRAALESSVTATAQRFGI
                     PYAGPGSAEPDAVAVDVPRPASWGGFRLIADAVELWVEGEFRIHDRACWTRSSTDAPW
                     SVTRLQP"
     gene            complement(3124..4014)
                     /locus_tag="VGE92_11000"
     CDS             complement(3124..4014)
                     /locus_tag="VGE92_11000"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY1314399.1"
                     /translation="MDRLDELSVFVAIVEAGSLVGAARRLRRSPPAVTRALADLENRI
                     GLRLVERTTRRLAPTEAGVKLAEGARTLLSDYDAALLGAAQAPVRGVLRITAPVQFGR
                     RHVAPIVSAFLNEYPEVSVELSLNDRNLDLIEEGLDLAVRIGPLADSSLVARQIASVR
                     RVVVASPAYLAAHGTPRVPTDLAAHDTIFGRARSPAREWRFGPPPRGAIVRLAPRLLV
                     DDVEAQLQAAQAGRGIARPLSYQVTEHLADGSLIRLLKEFEPEPLPVHLVTVSRSLLA
                     PKLRAFLDLAMKIFRHVEIR"
     gene            4118..4759
                     /locus_tag="VGE92_11005"
     CDS             4118..4759
                     /locus_tag="VGE92_11005"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY1314400.1"
                     /translation="MSASTEIVLHGTELSGHCHRVELLLRMLGLPYRYVLAPGSVRSS
                     AEFRKLNPLGQIPVLEDGSLILADSNAILVYLAKRYAPGGEWLPEEPAGAARVQRWLS
                     IAAGEVMYGPAIARMVCLWDFPGDLERAGRISARLLEFMNAHLQSRHFLAAEHCTIAD
                     LACYSYVAHAPEGGIPLDAYAAVRGWLRHVEALPHFKPMPAAPMPPSRLPAAG"
     gene            4767..5708
                     /locus_tag="VGE92_11010"
     CDS             4767..5708
                     /locus_tag="VGE92_11010"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY1314401.1"
                     /translation="MVALRFNADELAAQALAGGGPRGGGAIYDSMSEPHRLFFAQLRY
                     LLVGAVDAAGWPIATLLTGQPGFVQSADSATLRIGAQPAAGDPAAAALVAGHDIGILG
                     IDLSTRRRNRANGRVVGRDAQGIEVAVSQSFGNCPQYIQRRTLDAAQTAVGQAPASAA
                     ESFIHLDPAAQAAIEQADTFFVASRSRRAPPGDDGAYGADISHRGGRPGFVRVDGDEL
                     TIPDFRGNRYFNTLGNLMSEPRASLLFIDFDSGDLLQLQGLAYVDWTSAAAERFTGAE
                     RLWRFRVARAWRRRAASPLHWSFVDYSRVTQQTGAWH"
     gene            complement(5705..6763)
                     /locus_tag="VGE92_11015"
     CDS             complement(5705..6763)
                     /locus_tag="VGE92_11015"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEY1314402.1"
                     /translation="MSYINKPKVAHPSLPVNALGMTRRQYEGSMSTLCAGCGHDSVTA
                     ALVEACWGLSLRPEQLVKLSGIGCSSKTTSYFVSGGHGFNSVHGRMPSIASGANAANR
                     QLTYVGVSGDGDSLSIGLGQLVHAIRRNVNMLYVIENNGVYGLTKGQFSASADIGTKA
                     KKGDVNCSPPIDPVLLALTLGATFVARSFSGDKAQLVPLIQAGLRHNGFALIDVLSPC
                     VTFNDHEGSTKSYTFTREHYHPAIEADFVPRAQEIAVDYAAGESIPVSLHDGSRVVLR
                     KLDPTYDPTDRTAAYNYIGTKLKQNEYVTGLVHIDETDSTEFHNLNHTAATPLNKIPF
                     EKLSPGRAALDKLMARYR"
     gene            complement(6773..8617)
                     /locus_tag="VGE92_11020"
     CDS             complement(6773..8617)
                     /locus_tag="VGE92_11020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEY1314403.1"
                     /translation="MPGLTAPANRVNDFVIKFANVNGTGSASANSLVMKAIFRMGVPV
                     TGKNYFPSNIQGLPTWYEIRVTRDAYAARATQVDIMVAMNAETYSRDIRELGSGGYLI
                     YDSTWPRSSLLQRGDITVIEVPLARLVNENFEGVRNRILLKNIAYAGVLAALIDIDVA
                     VLGALLSESYGKKPKLLDSNTKALRLGYDYVKQHLTSPLPLRVEHMDKTRGHIMIDGN
                     TAAGLGCVYAGATVGAWYPITPSTSLMDAFKGFCERYRVDRESGEHNFAVLQAEDELA
                     AVGMVLGASWNGARAFTPTSGPGISLMNEFVGLAYYAEIPCVIFDVQRVGPSTGMPTR
                     TQQSDIMICAYASHGDTRHVLLFPANPEECFYMAVQAFDVAERLQTPVFVLSDLDIGM
                     NDWMCPDLKWDDAYRPDRGKVLSAEELAGLDKFYRYVDRDDDAITYRTLPGTSPKAAY
                     FTRGSGHNQYGGYTEDSAEYQVVLDRLKRKFANAPKYLPKPVTVGKGNSDVGIVSIGS
                     CDGAIREALDVLGRQKIDVDYLRVKAFPFNNEVESFLKAHSTIFVVEQNRDAQLKSLL
                     TLETAVEKSKLFSILSYSGFPMSAEPIVNGIRAKLEEAPRLTAISRKT"
     gene            complement(8605..>9504)
                     /locus_tag="VGE92_11025"
     CDS             complement(8605..>9504)
                     /locus_tag="VGE92_11025"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY1314404.1"
                     /translation="PKSFVTEDGRLLGMMFEKLEYDIENGDIAATRSLGDVFIACDDV
                     ILAIGQENAFPWIEKDIGIEFDKWNVPKVDKVTFQSTLPAVFFGGDAAFGPKNIIWSV
                     EHGHQAAISIHKFCHDEPVEERMARGVTLSSRKMGMHEWAYKNEYNGVERRLVPHVGL
                     VERFKKINIEVELGFTAEEAALEVQRCLNCDVQTVFAAKLCIECDACIDICPVDCLTI
                     TANGTETELRARLTAPAANRSQALYVSAPLPQTARVMVKDEDVCVHCGLCAERCPTAA
                     WDMQKSTIHIPLAIDEKVLACPA"
BASE COUNT         1714 a         3026 c         2967 g         1797 t
ORIGIN      
        1 cacggaggcc gctcgatctg gatcaatttg ccgccctgcc gggtgttggg caggcgaagc
       61 tcgcccgcta cggcgagcaa ttcgtcgccg cggtgcgcga acacggcgtc ccggcgccgg
      121 catagagcgc gccttcagcc ggcgcccacc tgcacacgat ttcgccccag cgccttggcc
      181 cgatacaacg ccgcatcggc gtcggccaag agtcgatctg cgaccggctt tagatccgtt
      241 tcgtgacgcc ccggcgcaac cgccgccacc ccgatcgaca ccgtcaaggt gcgctcgatc
      301 tgcgcggtca gggtgaacgg cgcagcggcg atggcctcgc ggattcgttc ggccagcgtc
      361 gtggcctcgg ccgcacctgc ctcgggtaag atgatggtca attcatcgcc cccaaaacgt
      421 gcggcggtat ccatgctgcg aatttgagat tccacacggt gggcaatttc ttttagtgca
      481 ttgtcgcccg ccaagtggcc gtagccgtcg ttgatgctct tgaagcggtc gatgtcgatc
      541 atcaaacagg cgatggagcc ggcgcgccgt tgtgcgcgcg ccaactcttc cttgagacgc
      601 tgctgcaagt agcggcgatt cgcccagccc gtcaggaaat cggtgatgcc ggcgcgcagc
      661 agacgtgtgc gattcaccgc attttcgacg cacacggcgg ccacggcgcc caaatgcgcc
      721 agaaaatccg tgccgtgatg gcgcgtgaag cgcgtgatgt cgcggctgcc gaagcacagc
      781 gccccggtcg cccggtcttt gcgggtcagc ggcacgaggg cggcgctctt gagattcgat
      841 gtgccgggaa acagcaggtg atggtcggcc ccgacgaacg ggcccaacca tggtttatgc
      901 agggcggtca gttgcggggc caaacccacc aacgaatcga cgaagaaaac ctgcttgaat
      961 tcctcggggc ggtcgccgcc ggccaacaac agatgacgaa cttcatgctg cggatcgagc
     1021 agcacgacat tcacggcgtc tagggcatag gattcgcgca gtccgtccac cattgcgtgc
     1081 aacagctgcg ccaaggtctc ggcgcgcaag agcgtcagtt cgcgcgcctg cgtgcgcttc
     1141 aagatggact cgttgttggc ggcttccgcg gtcaattccg tcaggcgctt tttgaggtcc
     1201 tcgttctcct ggatcacgtc ttccatgtcg gaaatcccca cgccgcgatc agccggcacg
     1261 cgcagctgcc cgcacgtcca agtacgctcg gccgcggttc atgagagcgc tgaattgtgc
     1321 cgaatccccg gccttcacgg ccgcgaccag acgttcgacg gcttgctgca gggcgagcaa
     1381 tgactcaccg ccgtactcgt tgaggctttg aatttcgaaa tacagttcgg gactctcggc
     1441 cgccaccctg ctcgccactt ccaattgcgc gtcgaaggtg gtgctcgaca attgcgcgag
     1501 tcggggagcc gcctcgccgc tatcggccag cgcggtgaag aatgcgatgt tgagcgcgtg
     1561 cgacaacccg agtacatagg cgatcaaacg atcgtgttcg tcgaggccca tcaccactct
     1621 ctcggccatg gtcggagcga acaattcctg agcctcctgc agggcttcgg gattcgccag
     1681 atcgatgaag atcacatgtc gtcccgagag caattcggtg tccggcccga acatcgggtg
     1741 cagagaggtg acgcggcagc ccgcttttga cagcgcctcg agaccggcgc gcaacggtgt
     1801 cttgagcgag cccaggtcga aaatcagtcc tcgcggccga cgctgcgcca ggaattcgag
     1861 caaccgcgta gtcgcgctca gcggtgtcgc cagcacgatg acatcgaagt ccaaggggtc
     1921 ggtctgccaa tcggcgatgg tatcgaaccc ggcaagaccg ccggcgggat cggcgatcgc
     1981 gacacgaaag ccttgcgatg ccaggaaatc cgcgaaccaa ccgcccattt tgccgtgacc
     2041 gccgatgacc agggccgttt tgccgctgcc atgggcctgc gccgcaactc gtgcgtgctc
     2101 ctgtgtcgca agcgatgact ggatcaactg gcgcaacagc gattcggcga gatcgggcga
     2161 aacgccgcgc cgcgccgcgg cattgcggcc gcgcaggatg acttcgcgtt cccggccgaa
     2221 gtcgcgggtc gggcgcccgg tggctcgttt gaccgcggcc acttgttcgc tcaatgcctg
     2281 ccggcgagcg accaaatcca cgagctgccc atcgagatcg gtcaacgcgg cgcgcaattc
     2341 atccagattc atgcagaaca atctcccatg gtcatttctc gttctactgg ccgcccagcg
     2401 ctaccgcaag cgtcgcggca cggctaccat tcaggggatg agcagcagca agacacggac
     2461 cgaaaccctg cccgaccccc tgcccgcgaa tcccctcgat gtcgcggccc agtggctcgc
     2521 ccaggcgagg ctcgatgcgg cccagcccaa ccccaattcc atggtcttgg cgacggtcga
     2581 tgcgcgcggt cacccctcgg cgcgtgtcgt actgtgcaag gaaatcagcc cggcgagcgg
     2641 ctccgtcgtc ttctatacca actatcgatc gcgcaaaggt cgcgagcttg ccggcaaccg
     2701 tcgggcggca gtggtgtttc actgggatca ccgccatcgc caggtgcgca tcgagggaca
     2761 gattgaacgg ctgtcggacg cggaaaatga cgcctatttc cagactcgtc catggcagag
     2821 ccggatcggt gcgtgggcca gcgaacagag ccaacccgtc gcatcgcgag cagccctcga
     2881 gagcagcgtc accgcgacgg cgcagcgctt cggaatcccc tacgcaggtc cgggttccgc
     2941 agaacccgac gctgtcgcgg ttgacgtgcc gcgtcccgct agctggggcg gatttcgttt
     3001 gattgccgac gcggtggagc tctgggtgga gggtgagttc cgtattcacg accgggcgtg
     3061 ctggacgcga tcctcgacgg atgcaccctg gtcggtcact cgactgcagc cgtgaggcgt
     3121 gagtcaccgg atctcgacgt ggcggaagat cttcattgcc aaatccaaaa aagcacgcag
     3181 cttgggcgcc aacaggctcc ggctcaccgt aacgagatgc accggcaacg gctccggctc
     3241 gaactccttc agcagccgga tgagtgagcc atcggccaag tgttcggtta cttgatagga
     3301 gaggggccgg gcgatgccgc gcccggcctg cgcggcctga agttgagcct ccacgtcgtc
     3361 gacgagcagg cgcggtgcga gccgcacgat ggcaccccgc gggggcggac cgaagcgcca
     3421 ctcgcgggcc ggcgatcgtg ccctgccgaa tatcgtatcg tgcgcggcga ggtcggtcgg
     3481 aacccgcggg gtcccgtgcg cagcgagata cgccgggctt gccaccacca cgcgccgcac
     3541 gctggcgatt tgccgcgcca ccaagctcga atctgccagc ggcccaatcc gcactgcgag
     3601 atcgagtccc tcctcgatga gatcgagatt gcggtcgttg agcgacagct ccacgctcac
     3661 ctccggatat tcgttgagaa aagccgacac gatgggcgcg acgtggcggc gcccaaattg
     3721 gaccggcgct gtaatccgca gcactccccg gaccggcgcc tgcgccgcac cgagcagcgc
     3781 ggcgtcgtag tcgctgagca gggtgcgcgc gccctctgca agcttgactc cggcttccgt
     3841 gggcgccagc cgccgggtgg tccgttcgac caaacgtaag ccgatgcgat tttccaagtc
     3901 ggccagcgcc cgcgtgacgg ccggcggcga gcgtcgcagg cgccgcgcgg cgcccaccag
     3961 actcccggcc tcgacaatgg ccacgaagac cgacagttca tccaagcgat ccatcgatta
     4021 ttccatttat aggaattctt agttactaaa actacatatt taccgtaaat ttggcaatag
     4081 tagagttgct tacgcgacca ctacttgggg agcgacgatg tccgcctcta ctgaaatcgt
     4141 tcttcacgga actgaattat cgggacactg ccaccgtgtc gagttgctgc tgcggatgct
     4201 gggtctgccg tatcgctacg tgctcgcccc gggaagtgtt cgatccagcg ctgagtttcg
     4261 caaactgaac cctttgggcc agatcccggt cctcgaggac ggctcgctga tacttgcgga
     4321 cagcaatgcc atcttggtgt atctggccaa acgctacgct ccaggtggcg agtggctccc
     4381 ggaggagccg gctggcgcgg cgcgcgtaca acgctggctg tcgattgcgg ccggagaagt
     4441 catgtacggt ccggcgatcg cgcgcatggt ttgtctatgg gattttccgg gtgacctcga
     4501 gcgggctgga cgaatctcgg cgcggctgct cgagttcatg aatgcgcatt tgcagtcgcg
     4561 tcattttctg gcggccgagc attgcacgat cgcggatctg gcctgctatt cctacgtcgc
     4621 gcatgcacct gaaggcggca ttccgcttga cgcatatgca gcggtacgcg gctggctgcg
     4681 ccacgtggaa gccctgccgc atttcaaacc catgccggcc gcgcccatgc cgccgtcacg
     4741 cctcccggcg gcgggttgag acggccatgg ttgcgttgcg gttcaacgcc gatgagttgg
     4801 ccgcgcaagc cctggccggc ggcggaccgc gcggcggcgg cgccatttac gactccatgt
     4861 cggagccgca tcggctcttc tttgcgcagc tgcgctatct cttggtcggc gcggtcgatg
     4921 cggccggctg gcctattgcg acgctgctga ccggacagcc aggttttgtg cagtctgcgg
     4981 attcggcgac gctgcgtatc ggtgctcagc cggcggcagg cgatccggcc gcagccgctc
     5041 tcgtcgccgg gcacgacatc ggcattctgg gcatcgatct gtcgacccga cggcgcaatc
     5101 gcgccaacgg ccgggtcgtc ggccgcgacg cgcagggcat cgaagtcgcg gtgtcccaga
     5161 gcttcggtaa ctgcccgcaa tacatccagc ggcgaacgct tgacgccgcg caaactgccg
     5221 tcggtcaagc gccggcgagt gcagccgaat ccttcattca cctggatccg gccgcgcagg
     5281 cggcaatcga gcaggccgac acctttttcg tcgccagccg ttcgcgccgc gcgccgcccg
     5341 gcgatgacgg cgcatatggc gcggatatct cacaccgcgg cggccggccc ggcttcgtgc
     5401 gggtcgatgg cgatgagttg acgattcccg actttcgcgg caaccgctac ttcaacacct
     5461 tgggcaatct catgtccgag ccgcgtgcgt cgttgctgtt catcgacttc gatagcggcg
     5521 acctgctgca gctgcagggc ttggcatacg tcgactggac aagcgcggcc gccgaacgat
     5581 ttacgggagc cgagcggtta tggcgttttc gcgtggcacg cgcttggcgg cgccgggctg
     5641 cctcgccgct gcattggtca ttcgtcgact attcgcgcgt gacgcaacag accggcgctt
     5701 ggcactagcg atagcgagcc atcagtttat cgagcgccgc ccggcccggc gacagcttct
     5761 cgaacgggat tttgttcagc ggcgtcgctg ccgtgtggtt cagattgtgg aactcggtcg
     5821 agtccgtttc atcgatatgc acgagaccgg tgacatactc gttctgcttg agcttggtac
     5881 cgatgtagtt gtacgcggcg gtccgatccg tcggatcgta ggtcggatcg agttttcgca
     5941 gcacgacccg gctgccgtcg tgcaggctga ccgggatcga ctcgccggcc gcataatcga
     6001 ccgcgatctc ctgcgcccgc ggcacgaagt ccgcctcgat cgccggatga tagtgctcac
     6061 gcgtgaaggt gtagctcttg gtcgaaccct cgtggtcgtt gaacgtcaca cacggcgaca
     6121 gcacatcgat caaggcgaaa ccattatggc gcagacccgc ctggatgagc ggcacgagct
     6181 gcgccttgtc gccggagaaa ctgcgtgcga cgaacgtcgc acccagggtg agcgccagaa
     6241 gcacggggtc gatcggcggc gagcaattga catcgccctt tttggccttg gtaccgatgt
     6301 cagcggacgc cgaaaactgg cccttggtga ggccatacac gccgttgttc tcgatcacat
     6361 acagcatgtt gacgttgcgg cggatcgcgt gcaccaattg cccgaggccg atggataggg
     6421 aatcgccgtc gcccgatacg cccacatagg tgagctgccg gttcgcggcg ttcgcgccgc
     6481 tcgcgatcga gggcatgcgt ccgtgcaccg aattgaatcc atgtcctccg ctcacgaaat
     6541 acgacgtggt cttcgatgaa caaccgatgc cggacagctt gaccagctgc tccgggcgca
     6601 gcgacagacc ccagcaagct tcgacgagcg ccgccgtgac cgaatcgtgg ccgcagccgg
     6661 cgcacaacgt cgacatgctg ccttcgtact ggcggcgcgt catgcccaag gcattgacgg
     6721 gcagcgatgg atgggcgact ttgggcttgt tgatgtagga catggggagc tctcaagtct
     6781 tgcgcgagat cgccgtcagc cgcggggcct cttcgagttt tgcgcggata ccgttgacga
     6841 tcggctcggc cgacatcgga aatccgctgt aactcaggat ggagaacagc ttggatttct
     6901 cgaccgcggt ttcaagcgtg agcagcgatt tgagctgcgc atcgcgattc tgttcgacca
     6961 cgaaaatggt cgaatgcgcc ttcaagaaag actcgacctc gttattgaaa gggaaggcct
     7021 tgacccgcaa ataatcgacg tcgatcttct ggcgcccaag cacatcgagc gcttcgcgaa
     7081 tggcgccatc gcaactgccg atgctcacga tcccgacatc gctgttgcct ttgccgaccg
     7141 tcacgggctt gggcagatac ttcggcgcat tcgcgaattt tcgcttcaac cggtcgagga
     7201 ccacctggta ttccgccgag tcctcggtat atccgccgta ttgattgtgg cccgaaccgc
     7261 gggtgaaata cgctgctttg gggctggttc ccggcagtgt gcgatacgtg attgcgtcgt
     7321 cgtcgcggtc cacgtagcga tagaatttgt ccaaccccgc gagctcttca gccgacaaga
     7381 ccttgcctcg atcgggccga taggcatcgt cccacttgag gtccggacac atccagtcgt
     7441 tcatgccgat gtccaggtcg gaaagcacga ataccggcgt ctgcaggcgt tcggcgacgt
     7501 cgaaggcctg caccgccata tagaagcatt cctcgggatt tgccggaaac agcagcacat
     7561 gccgcgtgtc gccgtgggaa gcgtaggcgc aaatcatgat gtccgactgc tgcgtacgcg
     7621 tcggcatgcc ggtcgaggga ccgactcgct gcacgtcgaa gatcacgcac ggtatctcgg
     7681 cgtaataggc caaaccgacg aattcattca tcaaggaaat gccgggcccg ctggtcggcg
     7741 tgaaggcgcg tgcgccgttc cacgaggcac cgagcaccat gccgacggcg gccaattcgt
     7801 cttcggcctg cagtaccgcg aaattgtgct cgccgctctc tcgatccacc cggtaacgct
     7861 cgcagaagcc cttgaacgcg tccatcagcg aggtcgacgg cgtgatggga taccaggcac
     7921 cgacggtcgc gccggcgtac acgcagccga gccccgccgc ggtgttgccg tcgatcatga
     7981 tgtggccgcg ggtcttgtcc atgtgctcga cgcgcagcgg cagcggcgaa gtcaaatgct
     8041 gtttcacgta gtcatagccc aagcgcagcg ccttggtgtt cgagtcgagc aatttcggct
     8101 tcttgccata gctctcgctc aatagagcgc cgagcacagc gacatcgatg tcgatcaatg
     8161 cggccagcac gccggcgtag gcgatgttct tcagcaggat gcgattgcgc acgccctcaa
     8221 agttctcgtt gaccagccgg gcgagcggca cttcgatgac cgtgatatcg ccgcgctgca
     8281 acagcgaact gcgcggccag gtcgaatcgt agatcagata gccgccggaa ccgagctcgc
     8341 ggatgtcgcg cgaataagtc tccgcgttca tggccaccat gatgtcgacc tgggtggcgc
     8401 gggccgcgta ggcgtcgcgc gtcactcgga tctcgtacca cgtgggcagg ccctgaatat
     8461 tcgaggggaa gtaattcttg ccggtcaccg gcacgcccat gcggaaaatc gccttcatca
     8521 ccagtgaatt ggcgctcgcc gagccggtgc cgttgacgtt ggcgaacttg atcacgaagt
     8581 catttacccg attcgcgggg gcagtcaggc cgggcatgcg agcaccttct catcgatcgc
     8641 gagcggaatg tggatcgtcg atttttgcat gtcccaagct gcggtcgggc agcgttccgc
     8701 gcacaagccg cagtgtacgc aaacgtcttc gtccttcacc atgacgcgcg ctgtttgcgg
     8761 cagcggcgcg gaaacataca gcgcctggga acgatttgcg gccggcgccg tcagccgggc
     8821 acgcagctcc gtctccgtgc cgttcgcggt gattgtgagg cagtcgaccg ggcaaatgtc
     8881 gatgcacgca tcgcactcga tacagagctt ggctgcgaac accgtttgca cgtcgcaatt
     8941 caagcagcgc tgcacctcga gcgccgcttc ttctgcggtg aacccgagtt ccacttcgat
     9001 gttgatcttc ttgaagcgct cgaccagccc gacgtgcggt accagccgcc gctccacacc
     9061 gttgtactcg ttcttataag cccattcgtg catgcccatc ttgcggctcg acaaggttac
     9121 gccgcgtgcc atgcgctctt cgaccggctc gtcgtgacag aacttgtgga tcgagatggc
     9181 cgcttgatgg ccgtgttcga ccgaccagat gatgtttttc gggccgaacg ccgcgtcgcc
     9241 gccaaaaaaa accgccggca gcgtcgactg aaaggtgacc ttgtcgacct tcggtacatt
     9301 ccacttatcg aactcgatgc cgatgtcctt ttcgatccac ggaaacgcgt tttcctggcc
     9361 gatcgcgaga atcacgtcgt cgcacgcaat aaacacatcg cccagcgagc gcgtcgcggc
     9421 gatgtcgccg ttctcgatgt cgtattcgag cttttcgaac atcatgccga gcagccggcc
     9481 gtcttcggtt acgaaagatt tggg
//
LOCUS       DASWZT010000391         2234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_70804, whole genome shotgun sequence.
ACCESSION   DASWZT010000391 DASWZT010000000
VERSION     DASWZT010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70804"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VGE92_11030"
     CDS             <1..330
                     /locus_tag="VGE92_11030"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY1314405.1"
                     /translation="RTLGLSPRGVIAVFMTQGVLIGWIGAAAGVGLGLALACNVDVIV
                     PFLEQTLGFHFMDPDVYYITGLPSEVHPGDVVRIGAAALLLTLAATIYPARQAAKTQP
                     AEALRYE"
     gene            327..1577
                     /locus_tag="VGE92_11035"
     CDS             327..1577
                     /locus_tag="VGE92_11035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEY1314406.1"
                     /translation="MMRSWYEGFVGLRYLRASPKRGSLSLIAGIAILGLCLGVAVLIV
                     VLSVMNGFEEVLRTRILSLTAHATISGLEGRISNWRGDLQKLQHFPGIAAAAPYIEEQ
                     GMLIHGDKSSGVLVRGVLPDAERRVVDLQQHLLSGRMSDLAAGSYRVILGQALAEELG
                     AKVGDRVVLLVALGDVTPVGVIPRMRSFEVAGILAIGMYEYDRRIAIVSMQDAAKLLR
                     MGDAVSGIRLSLTDMYAAPRISRAAAIALGGDLLVQDWTNEHANFFRSISITKRILFF
                     ILSLMVAVAAFNVVSTMVMVVKTKRRDIAILRTFGSSPRSILTIFVVQGSLIGVLGIG
                     AGVVLGVAIAANLQDLVHVLESIVGFKFLDARVYFMSDLPARVHAGDVARICGFAFVL
                     ACLSTLYPAVRAARLPPAESLRND"
     gene            1592..>2234
                     /locus_tag="VGE92_11040"
     CDS             1592..>2234
                     /locus_tag="VGE92_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1314407.1"
                     /translation="MSNSGEVVLKGEDLSKDFLQGDVKIQVLRAACISVRAGERIAIV
                     GASGSGKTTLLQLLGGLDLPTAGSVEIDGSQMNRLNDAERGYLRNRAVGFVYQFHHLL
                     PEFTALENVAMPLLVRRVEPRAAKQQAGALLARVGLGERLTHRPSQLSGGERQRAAVA
                     RALVTRPKLVLADEPTGNLDGANAAQVFDLMLELNRELGTSLVIVTHDERLAAR"
BASE COUNT          369 a          655 c          745 g          465 t
ORIGIN      
        1 cgcacgttgg gtttgagtcc gcgcggtgtg atcgccgtct tcatgacgca aggtgttctc
       61 atcggttgga tcggcgcggc cgcaggcgtc gggctcggcc tggcgctcgc ctgcaatgtg
      121 gatgtgatcg tgccgtttct cgagcaaacg ctcggcttcc atttcatgga tccggatgtg
      181 tactacataa cgggactgcc gagcgaggta caccccggcg acgtcgtgag aatcggcgcg
      241 gcggcgttgc tcctgacctt ggccgcgacg atctatcccg cccggcaggc ggcgaaaacc
      301 caaccggccg aagcgctgcg ctacgaatga tgcgctcatg gtatgaaggg ttcgttggat
      361 tgcggtattt gcgcgcatcg ccgaagcgcg gctcgctgtc cttgatcgcc ggcatcgcca
      421 tactgggcct ctgcctcggc gtcgcggtgt tgatcgtggt gctgtcggtc atgaatggct
      481 ttgaagaagt attgcgcacg cgaattctga gtctcaccgc ccacgcgacg atttccgggc
      541 tcgaggggcg gatatcgaac tggcgcgggg atcttcagaa gctgcagcac ttcccgggaa
      601 tagcggcggc ggcgccgtat atcgaagaac aaggcatgct gatccacggc gacaagtcgt
      661 ccggcgtgct ggtgcgcggc gtattgcccg acgccgagcg gcgcgttgtc gacctgcagc
      721 agcatctgtt gagcggccgc atgagcgatc tcgccgccgg ctcgtaccgc gtgatcttgg
      781 ggcaggcgct cgcagaagaa ctcggcgcca aagtcggcga tcgcgtggtg ctgctcgtgg
      841 cattgggcga tgtcacgccg gtcggcgtca tcccgcgcat gcgctcattc gaggtcgccg
      901 gcatcctcgc catcggcatg tacgaatacg atcgcaggat tgccatcgtc tcgatgcaag
      961 atgccgccaa gttgttgcgc atgggcgacg cggtgagcgg aattcgcctg agcctcaccg
     1021 acatgtatgc ggcaccgcgc atatcgcgcg ccgccgccat cgcgctgggc ggagatctgc
     1081 tggtgcagga ttggaccaac gaacatgcca atttctttcg ttcgatctcg atcaccaagc
     1141 gcatcttgtt cttcattttg tcgctgatgg tggcggtcgc tgcattcaac gtggtatcga
     1201 cgatggtgat ggtggtgaag accaagcggc gcgacattgc catattgcgc accttcggca
     1261 gctcgccgcg aagcatcttg acgatattcg tcgtgcaggg cagcttgatc ggcgtgctcg
     1321 gcatcggtgc cggcgtagtg ctcggtgtcg ccattgcggc gaatctgcag gatctcgtgc
     1381 atgtcttgga aagcatcgtc gggttcaagt ttctcgacgc ccgtgtgtac ttcatgagcg
     1441 acctgccggc gcgggtgcat gccggcgacg tggcccgcat ttgcggattc gcgtttgtgc
     1501 tcgcctgcct ctcgactctt tatccggcgg tgcgcgctgc gcgcctgccg cctgcggaat
     1561 cgctgcgcaa cgattgagga tggaaaagac catgtcgaat tcaggcgagg tggtactcaa
     1621 aggcgaggat ctttccaagg atttcctgca gggcgatgtc aaaattcagg tgctgcgtgc
     1681 ggcctgcatc agcgtgcgag ccggcgagcg cattgccatc gtcggtgcgt ccggctccgg
     1741 caagacgacg ctgctgcagt tgctgggggg gctcgattta ccgaccgcgg gcagcgtgga
     1801 aatcgacgga tcgcagatga atcgattgaa cgacgcggag cgcggctacc tgcgtaatcg
     1861 cgccgtgggt ttcgtgtatc aattccatca tttgctgccg gaattcaccg cgctcgaaaa
     1921 cgttgccatg cctctgttgg tgcggcgcgt cgagccgcgc gccgccaagc agcaggcggg
     1981 cgcattactc gcgcgggtcg ggttggggga acgcctcacg catcgccctt cgcagttgtc
     2041 cggcggtgaa aggcagcgcg ccgccgtggc gcgcgcgctc gtgacgcggc ccaagctggt
     2101 attggccgac gaaccgaccg gtaacctcga tggcgcgaat gccgctcagg tcttcgatct
     2161 gatgttggaa ctcaatcgcg aattggggac cagcctcgtg atcgtgacgc acgacgaacg
     2221 attggcggcg cggc
//
LOCUS       DASWZT010000392         9857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_27285, whole genome shotgun sequence.
ACCESSION   DASWZT010000392 DASWZT010000000
VERSION     DASWZT010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9857
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27285"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1673)
                     /locus_tag="VGE92_11045"
     CDS             complement(42..1673)
                     /locus_tag="VGE92_11045"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEY1314408.1"
                     /translation="MRQLRCVMAQLNLVVGDVDGNTSRIVAAAVAARDQHRADALMVP
                     ELAVSGYPPEDLLFHSGMRLQVGRSLERLQQEVRGITLIAGYPEYVGTQIFNSAVVIR
                     DGVILANHRKACLPNYRVFDEKRYFTPGTEPTVVELNGLRAGVLVCEDVWDAEPARRA
                     RAAGAEVLLVINASPYEVDKQTQREQEIVRARCAESGIPLVFVNLVGGQDELVFDGNS
                     FVMDAQGNVTLRAPAFTEGLYVVDLVADPKGRVRPLPGHIVPLQGQEESVFGALVQGT
                     RDYVDKHRFPGVVLGLSGGVDSALTLVLAVDALGADRVHSVAMPSRYTSQMSKDDAAL
                     QAKWLGVKHSEISIEAMFESTLAALKDEFAGRAPDTAEENIQSRCRGVLLMGISNKTG
                     RMLLTTGNKSEMAVGYATLYGDMAGGFAPIKDCSKLLVYRLCAWRNAQSQVIPRRVID
                     RPPSAELRYGQKDTDSLPPYEILDPILEAFIEEDLSVDQITARGFDRATVGRILDLVK
                     RNEYKRRQAPPGVRVSSRAFGRDWRYPITSGYKTR"
     gene            complement(1828..2685)
                     /gene="sucD"
                     /locus_tag="VGE92_11050"
                     /pseudo
     CDS             complement(1828..2685)
                     /gene="sucD"
                     /locus_tag="VGE92_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016393102.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
     gene            complement(2682..3851)
                     /gene="sucC"
                     /locus_tag="VGE92_11055"
     CDS             complement(2682..3851)
                     /gene="sucC"
                     /locus_tag="VGE92_11055"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEY1314409.1"
                     /translation="MNLHEFQSKQLFAEYAIPIPKGQVASSAAAAVAAAEHLGGSLWV
                     VKAQVHAGGRGKAGGVKVARDLDAVRAAAEAMLGTVLTTHQTGPEGLPVHQVYVEVGS
                     KIEREIYLSLVLNREAGRIAFVASAAGGMDIEEVAAHTPEKIMRVNIHPTAGLQDFQA
                     RQLAFGLNLSGAQIAQFGKIAHALYSLYLERDASLVEVNPLIVTGDGDLMALDAKINI
                     EDNALFRHKDLAAMRDDSQEDPMERKAAQYDLNYVSLDGNIACMVNGAGLAMATMDLI
                     QLHGGAPANFLDVGGGATKERVAAAFKLILSNPRVTAILINIFGGIVRCDMIAEGIIA
                     AVKEVGVSIPVIVRLEGTNAPLARELLAESGLAITSARDLTDAAKKAVSLAGTRA"
     gene            3940..5556
                     /locus_tag="VGE92_11060"
     CDS             3940..5556
                     /locus_tag="VGE92_11060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY1314410.1"
                     /translation="MNNQLDRGVGSQATFSWRVLGLLNLYRVLVPLVLLGLYSLGGAR
                     GFGVTSPQVFFGAAVFYLCFGLGNVILVRRRLASIAVQTLLQASVDVTVSILLLHVWG
                     GVASGLGLLFLLPVGGLAFLLTPRSALFLAAIATLAVLGDTIWQQLSGHTDITSYATA
                     GLLGLVLFTTAAAASFVALRMRESEALVRQKDVDLANLAELSQYIVQHLRESLLVVDG
                     SDKIRLINESAAAILGDEQAVPGALVGEVSPRLLYSLTTWRQIERGEVSPSTFVAADG
                     ARVIQPHFAPLGDIAPGPTLIFLEDTSLMAERVQQGKLAALGRLSASIAHEIRNPVGA
                     MSHAGQLLAESPAIGPDERRLTDIIRNNSERVSTIINNVLQLSRREATKPTRLSLGDW
                     IGEFLAEFCETMQVPRTQIALVEEALELEVRFDPSHLHQVVWNLCDNAIKYGEARAGI
                     RVDIRLGRLSPSNRPFLEVADRGPGIEHTAVDRIFEPFFTGRKGGTGLGLFIARELCQ
                     LNRAILLYAPRSGDGSVFRIVFSDPQRWEG"
     gene            5577..6914
                     /locus_tag="VGE92_11065"
     CDS             5577..6914
                     /locus_tag="VGE92_11065"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY1314411.1"
                     /translation="MAKPAVLVVDDEPDLCELLTITLQRMDMSPRTANTVAAAKRMLR
                     SEAYDLCLTDMQLPDGDGLELVEWIQQYCSAVPVAVITAHGNVETAVRALKLGAFDFV
                     SKPLDLTGLRKLVATAIKLSEDGSGDTSVFGPRLLGNSRAMQDMREMIGRVARSQAPV
                     HIFGESGTGKELVAKLIHESGPRRDGPFVPVNCGAIPTELMESELFGHKRGSFTGAVG
                     DKKGLIPSAEGGTLFLDEIADLPLHMQVKLLRVIQEKAVRPVGEQLEVPVDVRVLSAT
                     HKNLAQLVVEGNFREDLFYRVNVIELRVPSLRERAEDVPELAEAILRRLGRRMKITPP
                     MLSKDAMTALESYAFPGNVRELENILERAITLSTGGEVRVGDIDLRPVPGGAPLAAGA
                     ANAGALGEQLEDIEREAILKALEQSKYNKTAAAKALGMSFRALRYRIKKLGIE"
     gene            complement(6919..8826)
                     /locus_tag="VGE92_11070"
     CDS             complement(6919..8826)
                     /locus_tag="VGE92_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY1314412.1"
                     /translation="MILQWFNAREAAEAGSEFADQLAPPSQPNVVVEAKAVQELLRRA
                     DLEVRPLRLNVYKRAKFANAFKWRLVANGVDAKLADELTQKLVLHLFLSQGAAAGRGA
                     AAGSTGGPGAGHGQQLFRHGNKHFEKGAYSEAVAAYEGFIKLHPRNADAFNNLGAALC
                     KLGRYKEAEAQFRKAIELKPESPDAHCNLGNVLRWTGRIAESENSLRRALKLKPTYAE
                     ARASLGLTLLLAGHLRDAKARFDKVLKSAPRNTDALLGAGQIAKMEGRFDDAAIMFKR
                     ALEVNPQMPSAWAAEVGLRKMTSADASWLQSAQQIAASGIAPLEEADLRFAIGKYCDD
                     TDDFEKAFRSYERGNKLLKGVAEDYNRAGRTALIDDLIRVYTREALSRAQAGASTSTK
                     PIFVVGMMRSGTSLAEQIISSHPLAKGAGELEFWGDAAREHDAEIRRGQLSAPQRKSL
                     AEAYLRTLTRDFADAVRVVDKAPTNADRLGLIHSVFPNARIIYMQRDPIDTCLSCYFQ
                     QFSTALNFTLDLSDLAHYFREHQRLVAHWRAVLPPGSMLDVPYEELVTDQPTWTRKIL
                     QFLDLEWDDRCLDFHKTKRSVVTASSWQVRQKIYAHSVGRWRKYEKFIGPLLQLRELE
                     PVNSRVAGSRR"
     gene            9177..>9857
                     /locus_tag="VGE92_11075"
     CDS             9177..>9857
                     /locus_tag="VGE92_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314413.1"
                     /translation="MLVALWTITHRYRGLSGDAELYALQALARIHPSLAADLYLQNVS
                     QDKFTIFSPLYTACIDLLGMHAAALTLTLCFTIWFFLASWFLARELTDGATAYLAVGM
                     LIITAGSYGGFGVFNYYEDWVTARSLGEALALTALALHFRGARRLALLLALGACFVHP
                     LMVLPCLLLLICLWLPVRASASAALLGLLCVPIVAAAALIGHATTGPLAVIDGDWLEV
                     VRERSQFLF"
BASE COUNT         1755 a         3136 c         2944 g         2022 t
ORIGIN      
        1 tcttgccgaa gaagcactgg tggagcctgc tgtaagcgtg atcagcgcgt cttatagccg
       61 ctggtgatcg gataacgcca atcccgcccg aatgcccggc tgctgacgcg cacacccggc
      121 ggcgcctgcc gccgcttgta ctcgttgcgc ttgaccagat ccaggatgcg cccgaccgtc
      181 gcccggtcga atcctcgcgc cgtgatctga tcgaccgata aatcctcctc gatgaaggcc
      241 tcgaggatgg ggtcgagaat ctcataaggc ggcaggctat cggtgtcctt ctgcccgtag
      301 cgcagctccg ccgagggcgg ccgatcgatc acgcgccgcg ggatcacctg cgactgcgca
      361 ttgcgccaag cgcacagccg ataaaccagc agcttgctgc agtccttgat gggcgcgaag
      421 ccgccggcca tgtcgccgta aagcgtcgca tagccgacgg ccatctcgct cttgttgccc
      481 gtggtcagga gcatcctacc ggtcttgttc gaaattccca tcagcaacac gccgcggcag
      541 cgcgactgga tattctcttc ggccgtgtcg ggcgcgcgcc cggcgaactc gtctttgaga
      601 gcggccaggg tcgattcgaa cattgcctcg atcgaaatct cgctgtgctt cacgcccagc
      661 cacttcgctt gcagcgcggc gtcgtccttg ctcatctgcg aggtatagcg cgaaggcatc
      721 gccaccgaat gcacccgatc cgcgccgagc gcgtcgaccg ccagcaccaa ggtcagcgcc
      781 gaatcgacgc cgccggataa gccgagcacc acgccgggaa atcgatgctt atccacgtag
      841 tcgcgggtgc cttgaaccag ggccccgaac acgctttctt cctggccctg cagcggcacg
      901 atatgccccg gcaggggcct gacccgccct ttagggtctg cgaccaaatc caccacgtac
      961 aggccttcgg tgaacgcggg ggcgcgcaag gttacgttgc cctgcgcgtc catcacgaag
     1021 gaattgccgt cgaacaccag ttcatcctga ccgccgacca gattgacgaa taccaacggt
     1081 attcccgatt cggcgcagcg cgcgcgcacg atttcctgct cgcgctgagt ttgtttgtcg
     1141 acttcatagg gcgaggcgtt gatgaccaac aacacttcgg cgcctgccgc gcgtgctcga
     1201 cgcgccggct cggcatccca cacgtcctcg cacaccaaca cgcccgccct caagccattc
     1261 agttcgacga ccgtgggttc ggtacccggc gtgaagtagc gcttctcgtc gaacactcga
     1321 tagttgggca gacaggcttt gcgatgattc gcgagaatca ccccgtcgcg gatgaccact
     1381 gccgaattga aaatctgcgt ccccacgtat tcagggtagc cggcgatcaa ggtgatgccg
     1441 cgcacctcct gctgcagacg ctcgagcgaa cgccctactt gcaggcgcat cccggaatga
     1501 aacagcaagt cctccggcgg atatcccgac acggctagtt ccggcaccat gagcgcgtcc
     1561 gcgcgatgct gatcgcgggc cgcgaccgcc gccgcgacga ttcgcgacgt gttgccgtcc
     1621 acatccccga cgaccaaatt cagctgcgcc atcacgcaac gcaactgccg catctcagtc
     1681 aattggcttc ccctatttcc ggcctcgtgt ggcggcccgc tttttggccg gctttcgcgc
     1741 cggccgctcg gccgcttgct tcgcaacctt ctgcgccggc ttgccgaccg gcttgccaac
     1801 cgccttgccg ggcttgctgt gcaatcgttt ggcgatggca gaccccaatt ccgccggcga
     1861 gcgcacggtc gtgatccccg cggcctcgag agccgcaaat ttcgcggccg ccgtgcccat
     1921 gccgccggcg acgatggccc ccgcatggcc catgcgcttg ccaggaggcg ccgccatgcc
     1981 cgcgatgtac ccgaccacgg gcttgctgac gttcttgcgt atgtaggccg ccgcggcctc
     2041 ctcgtcgctg ccgccgattt cgcccaccat cacgatgcct tcggtctgtg catccttctc
     2101 gaagagttcg agcacatcga tgaagttcat gccccggacc ggatccccgc cgatgccgac
     2161 gcaggtgctc tgtcccaggc gattgttggt ggtttggaat acggcttcat aagtcagggt
     2221 gccggaacgc gaaacgatgc ccacccggcc cttctgatgg atgaatccgg gcatgatgcc
     2281 gatcttgcac tccccgggcg tgatgatgcc cggacagttc gggccgatga gtctggtctt
     2341 gtagctcgcc agagtcattt tcacgcgcac catgtcgttc acgggaatcc cttcggtgat
     2401 gcacacgatg agttccaccc ccgcgtccgc cgcctcgagg atggcgtcgg ccgcgaacgg
     2461 cgccggcacg tagatcatgc tcacggtcgc gccggtgcgc gccaccgcct ctttggcggt
     2521 atcgaacacc ggaagtccca gatgctgctc tccgccccga cccggcgtta cgccgccgac
     2581 cacctgggtg ccgtaggcga tgcattgctc cgaatggaac gtgccctgtt tgccggtaaa
     2641 gccctgacaa atcacccggc tgcctttatt cacgaggatg ctcatgcgcg agttcccgcc
     2701 aggctcacgg cctttttcgc tgcatcggtc aggtcgcggg ccgaagtgat ggcaaggccg
     2761 ctctcggcaa gtaactcccg cgccagcggc gcgttggtgc cctcgagccg cacgatcacc
     2821 ggaatgctga cacccacctc tttgacggct gcgatgatgc cctccgcgat catgtcgcaa
     2881 cggacgatac ccccgaagat gttgatgagt atcgcggtca ctcgcgggtt cgacaggatc
     2941 agcttgaaag cggcggcgac tcgctctttg gtggcgccgc cgccgacgtc cagaaagttg
     3001 gccggcgcgc cgccgtgcaa ctggatgagg tccatggtcg ccatggcgag cccggcgcca
     3061 ttgaccatgc aggcgatatt gccgtcgagc gacacgtagt tcaggtcata ttgcgccgcc
     3121 ttgcgctcca tcgggtcttc ttgggagtca tcgcgcatgg ccgcgagatc cttgtgccgg
     3181 aacagggcgt tgtcttcgat attgatcttt gcatcgagcg ccatcagatc accgtcgccc
     3241 gttacgatca gcggattgac ttccaccaag ctcgcatcgc gctccaaata gagcgagtag
     3301 agggcgtgcg cgattttccc gaattgcgcg atttgcgcgc cgcttagatt caagccaaac
     3361 gccaactgcc gcgcttgaaa gtcctgcagg cccgcggtcg gatgaatgtt gacccgcatg
     3421 atcttctccg gggtgtgggc ggcaacctct tcgatgtcca tcccgcccgc cgcggaggcg
     3481 acgaaggcga tgcgacccgc ttcgcgattg agcaccaagc tcaggtatat ctcgcgttcg
     3541 atcttcgatc cgacctcgac atacacttgg tggaccggaa gtccctcggg tccggtttga
     3601 tgggttgtca ggacggtccc gagcatcgcc tcggccgccg cgcgcaccgc atccagatca
     3661 cgggcgacct tgacgccgcc cgctttaccg cggccgcccg cgtgaacctg ggctttcacc
     3721 acccacagcg agcctcccaa gtgctccgcg gcggcgaccg ccgccgccgc cgatgacgcg
     3781 acctgccctt ttgggattgg gatcgcatac tcggcgaata gctgttttga ctgaaactcg
     3841 tgcaaattca tgaattgtcc tgcaatgcgg atccggtcac gtaaggcggt ctattctgaa
     3901 ttaagcttaa ctgctgcgca aagccaggga caaacatctt tgaataacca attggaccgg
     3961 ggcgtcggct cgcaagccac ttttagttgg cgagtgctcg gtttgctcaa tttatatcgc
     4021 gtcttggtgc cgctggttct gctcggcctt tattcgttgg gcggtgcacg cggtttcggt
     4081 gtcacctcgc cgcaggtgtt tttcggcgcc gccgtgtttt acctgtgttt cgggctcggc
     4141 aacgtgattt tggtgcgccg acggcttgcc agcattgcgg ttcaaaccct gttgcaggcg
     4201 tccgtcgacg tgaccgtctc gatcctgctg ctgcacgttt ggggcggtgt cgcgagcggt
     4261 ctcgggctgt tgttcctgct tccggtcgga ggcctcgcgt tcctcttgac gccgcgcagt
     4321 gcgctgtttc tggcggccat cgcgacgctt gccgtactcg gcgacaccat ttggcagcag
     4381 ctttccggtc ataccgacat tacttcgtat gcaaccgcgg gtctcttggg gctggtcctg
     4441 ttcaccacgg cggctgcggc cagcttcgtc gctctcagaa tgcgcgagag tgaggccttg
     4501 gtccggcaaa aagacgttga cctggcgaat ctcgccgagt tgtcgcagta catcgtgcag
     4561 cacttgcgcg aaagcctgct ggtggtcgat ggctccgaca aaatccggct catcaacgag
     4621 tcggcggcgg cgattctggg cgatgaacaa gcggtaccag gggccctggt gggtgaggtt
     4681 tcgccgcgcc tgctgtactc cctgaccacc tggcgccaga tcgaacgcgg cgaagtgtcg
     4741 ccgtcaacct tcgtggcagc ggatggtgcg cgggtgattc aaccacactt cgcgccgctc
     4801 ggtgatatcg caccgggacc tacattgatt ttccttgaag ataccagtct catggccgag
     4861 cgtgtgcagc aaggcaagct cgccgcgctc ggccgtctgt ccgccagcat cgcgcatgaa
     4921 atccgcaacc ccgtgggcgc catgagccat gccgggcagt tgctcgccga gagtccggcc
     4981 atcgggccgg atgaacgccg gctgacggac atcattcgca ataactccga gcgcgtcagc
     5041 acaatcatca ataacgtcct gcagctttcg cgccgcgaag ccaccaagcc gacgcgcctg
     5101 agcttaggtg actggatcgg ggaattcctc gccgagttct gcgagaccat gcaagttccg
     5161 cggacgcaaa ttgccttggt tgaggaagcg ctcgaattgg aagtgcgctt cgaccccagt
     5221 catttacatc aggtcgtttg gaatctctgc gacaacgcca tcaagtacgg cgaagcgcgc
     5281 gccggcatca gggtcgacat caggctcggg cgtttgagtc ccagcaaccg accttttctc
     5341 gaagtggcag atcgaggacc cggaatcgaa cacacggccg tcgaccggat cttcgaaccg
     5401 ttcttcacgg ggcgcaaggg cggcacgggg cttgggctgt tcatcgcgcg cgagctgtgc
     5461 cagttgaacc gcgccatcct gctgtacgca ccgcgcagcg gcgatggcag cgtgttcagg
     5521 atcgtgttca gcgacccgca gcgctgggaa gggtagtcag cagctacaat gcgccaatgg
     5581 ccaaacccgc tgtcctagtc gtcgacgacg aacccgatct ttgcgagctg ctgacgatca
     5641 ccttgcagcg catggacatg agtccgcgca ccgcaaacac ggtcgcggcc gcgaagcgca
     5701 tgctcagatc cgaagcatat gatctgtgtt tgaccgacat gcaattgccg gacggcgacg
     5761 gcttggaatt ggtcgaatgg atccagcagt attgctccgc cgttcccgtc gcagtcatca
     5821 cggcgcacgg taacgtcgaa accgcggtgc gcgccctgaa gctgggcgcg ttcgacttcg
     5881 tctccaaacc gctggatctt accgggctgc gtaaattggt cgccaccgcc atcaagctgt
     5941 cggaagacgg cagcggcgat accagcgttt ttgggccgcg cctgctcggc aattcacgcg
     6001 ccatgcaaga catgcgcgag atgatcgggc gcgtcgcgcg cagccaagcg ccggtgcaca
     6061 tctttggcga gtccggaacc ggtaaggagt tggtcgccaa gctcattcat gaatccggac
     6121 cgcgtcgcga cggacctttc gtgccggtca attgcggagc gattcccacc gagctcatgg
     6181 agagcgagct gttcggccac aagcggggta gtttcaccgg cgccgtcggc gacaagaagg
     6241 gcctgattcc gtcggccgaa ggcggcacgc tgttcttgga cgagatcgcg gacttgccgc
     6301 tgcacatgca agtcaagctg ctgcgcgtga tccaggagaa ggccgtgcgc ccggtcggcg
     6361 aacaactcga agtgcccgtc gatgtgcgcg tgctgtccgc cacgcacaag aacctggcac
     6421 agttggtcgt cgagggtaat ttccgcgaag acctgttcta tcgggtcaac gtcattgaat
     6481 tgcgggtacc gagcctgcgc gagcgggccg aggatgtgcc tgaactagcc gaggccattc
     6541 tgcgcaggct tggcagacgc atgaagataa cgccaccgat gctgagcaag gatgccatga
     6601 cggctctcga gtcctatgcg ttccctggca acgtgcgcga actcgaaaat atcttggagc
     6661 gcgccattac gttgagtacc ggcggcgaag tgcgcgtcgg cgacatcgac ttgcggccgg
     6721 tgcccggagg cgcgccgctc gctgccggcg cggcgaacgc gggcgcactc ggtgagcagc
     6781 tcgaggatat cgagcgcgaa gcgattctca aagcgctcga acagagcaag tacaacaaga
     6841 ccgccgccgc caaggcgctc ggcatgtcct ttcgggcgct gcgctaccgg atcaagaagc
     6901 tggggatcga gtaacccgtc acctgcgact gccggccacg cgcgagttca cgggttcgag
     6961 ttcgcgtaac tgcagcaagg ggccgatgaa cttttcgtat tttcgccagc ggcccaccga
     7021 atgggcatag atcttctgac gtacctgcca cgagcttgcg gttaccacgg agcgtttcgt
     7081 cttgtggaaa tccaggcacc gatcgtccca ctcgagatcc aggaattgca atatcttgcg
     7141 cgtccatgtc ggctgatctg tcaccagttc ttcatatggc acgtcgagca tcgaacccgg
     7201 cgggagaact gcgcgccagt gcgcgaccag acgttgatgc tctcggaaat aatgcgccag
     7261 gtccgacaaa tccagggtga aattaagggc cgtcgagaac tgctggaaat aacaagacag
     7321 gcaggtatcg atcgggtctc gctgcatgta aatgatgcgc gcattcggga acaccgaatg
     7381 aatcaagccg agacggtcgg cgttcgtcgg tgccttgtcg accacgcgca cagcgtcggc
     7441 aaaatcacgc gtcagcgtgc gcaggtatgc ttcggccagt gattttcgct gtggcgcgct
     7501 caactgtcct cgccgaatct cggcgtcgtg ctcgcgtgcc gcgtcgcccc agaattccag
     7561 ttccccggcg cccttcgcca atggatgcga agatatgatt tgctcggcga gcgaagttcc
     7621 cgaacgcatc ataccgacca cgaatatcgg cttggtcgag gtcgaagccc ccgcttgcgc
     7681 gcgggacaat gcctcgcgcg tataaacgcg aatcaagtcg tcgatcaaag ccgtgcgccc
     7741 cgcgcggttg tagtcttcgg ccaccccctt gagcaacttg ttaccccgct cgtagctgcg
     7801 gaacgccttc tcgaaatcgt cggtatcgtc gcagtatttg ccgatcgcga agcgcagatc
     7861 cgcttcctcg aggggtgcga taccgctcgc cgcaatctgc tgcgcactct gcagccacga
     7921 ggcatctgcg gacgtcatct tgcgcaggcc aacctcggct gcccatgcgc tcggcatttg
     7981 cggattgacc tcgagcgcgc gtttgaacat gatcgcggca tcgtcgaatc ggccttccat
     8041 cttcgcgatc tggccggcgc cgagcagcgc gtcggtattg cgcggcgccg acttcagcac
     8101 cttgtcgaag cgagccttgg catcacgcag atgtccggct agaagcaaag tcagccccag
     8161 actcgcccgc gcctccgcgt aggtgggttt caatttgaga gcgcgccgca gcgaattctc
     8221 cgactcggcg atacgcccgg tccagcgaag cacatttccg aggttgcaat gggcgtcggg
     8281 actttccggc ttcaattcga tcgccttgcg gaactgcgcc tccgcctcct tgtagcgacc
     8341 caacttgcag agcgctgcgc cgagattgtt gaaggcgtcg gcgttgcggg gatgaagctt
     8401 gatgaagccc tcatacgcgg cgaccgcttc gctataagcg cctttctcaa aatgtttgtt
     8461 accgtggcgg aaaagctgct ggccatggcc cgcgccgggc ccgccagtcg atccggccgc
     8521 cgcaccgcgg cccgcggcgg ctccttgact caagaacagg tgcaatacca atttctgggt
     8581 aagctcgtcg gccaactttg catcgacgcc gttcgcgacc aatcgccatt tgaaggcgtt
     8641 ggcgaacttt gctcgtttat acacattgag tcgcaacggg cgcacttcga ggtcggcgcg
     8701 ccgcaggagt tcctggacgg ccttggcttc cacgacgaca ttcggctgcg acggcggcgc
     8761 caattggtcg gcgaattcgc tgccggcttc ggccgcttcg cgggcgttga accattggag
     8821 aatcatcgta tgttttccat gctcgacttc cggcagccgg cacaaaggtt cttcagacga
     8881 acagcgggcg tcgctaagct tctcataggt cgccctttaa tcctaacctt tccacgttcg
     8941 aagctgctcc gtcattctat tcacagtcga caaattccgt cgcgcccagg gcatccatgc
     9001 gattgcgcat cggatcacac atggccgatt atatactcga cataatccat cgaatgctac
     9061 caagttggca gaaatcagtg accgtgcgaa ggctgaacag tcgatccccc ctgcagcaat
     9121 cgccggattt cacggctggc tctgtgccgc tcgcgacagc gattgcgctg acgctcatgc
     9181 tggtcgcact ttggacgatc acgcatcgtt atcgaggtct ttccggagat gcggagcttt
     9241 atgccctaca agcgctggcg cgaatccatc cgagccttgc ggcggacctt tatttgcaaa
     9301 acgtttcgca ggacaaattc acgatatttt ccccgctcta caccgcgtgt atcgatttgc
     9361 ttggcatgca tgcggcagcc ctcaccctga cgctgtgctt cacaatttgg ttctttctcg
     9421 cttcgtggtt tctcgcgcgc gaattaactg acggcgctac agcttatctc gcggtcggca
     9481 tgctcatcat caccgccggt agttacggtg gctttggcgt gttcaattat tacgaggatt
     9541 gggtgactgc aaggtcgttg ggtgaagcgc tcgctttgac ggcgcttgct ctgcattttc
     9601 gcggcgctcg gcgcctcgct ctcttgcttg cgctgggcgc gtgtttcgtg catccgctca
     9661 tggtactgcc gtgcttgctg cttttgattt gcctatggtt gccggttcgt gcaagcgcga
     9721 gcgctgcgct gctgggattg ctctgtgttc cgatagttgc cgcggcggcg ctgatcggcc
     9781 atgccaccac gggcccgctg gcggtcatcg atggcgattg gttggaggtg gtcagggagc
     9841 gatcgcagtt cctattc
//
LOCUS       DASWZT010000393         6066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_75526, whole genome shotgun sequence.
ACCESSION   DASWZT010000393 DASWZT010000000
VERSION     DASWZT010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75526"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VGE92_11080"
     CDS             complement(<1..353)
                     /locus_tag="VGE92_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314414.1"
                     /translation="MQSELRLILLCLSVPLLAGIWWWSARRSSQARGNAELRESSVPL
                     EPRYTEPNDEGMRAQPTSREWGVPPFEPLNIRTGDFDHVPVLDGPMMVDLDPVAPPPP
                     AAMAVTDAAVAPFASA"
     gene            complement(365..3868)
                     /gene="smc"
                     /locus_tag="VGE92_11085"
     CDS             complement(365..3868)
                     /gene="smc"
                     /locus_tag="VGE92_11085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEY1314415.1"
                     /translation="MRLTKLKIAGFKSFVDPTTVSFPSSLTGVVGPNGCGKSNIIDAV
                     RWVMGEISAKHLRGESMADVIFNGSSARKPLGAASVELVFDNSDGKIGGEYAAYNEVA
                     LRRAVSRDGTSDYYINGTKVRRKDITQLFLGTGLGTRSYAIIEQGMISRVIEARPDDL
                     RAFLEEAAGISRYKERRRETENRIGHTRENLDRLNDLREEVEKQIRHLQRQAATARRY
                     QALKEEERKLQAELLALRLQSLDAEAGTRDAATSSCETAMQGVLADLRAAEAEIERIR
                     ADQAHKADALGAIQSRFYEAGGEVTRIEQSIQFTRELRQRQRADLEQAEAQVAELGQV
                     LQRDRGQIEELSQELTTMVPGLDAAHADEARTSEALASCEHALAAWQQTWDAHAQAVA
                     LGQGETGAERARIEQLEGEQRRLLQQRERQESERAQLMQLQPPVALETLQAQAQLARD
                     AGEKAATELQELLAEIAVSRDREREQSLALNELRGRWQEALGAQVSTEALQQAALGKV
                     SGKVSEWLKSNSLDRQPRVAQQLRVERGWERAVETVLGSYLEAVCVDGLDSVTDLLAS
                     FDGAHLAVVSMSEARTAPLAPASLQAKVQGATVLSSVLSSVYTAETLNEALRQRARLA
                     AGESVVTRDGIWLGQDWLRLSRDADPHTGVIEREESLRELHARVGALADEVKGLERKL
                     ELTREHLRDHEDRREQFQANVNRLHREHVDRRAAFDSAQARSSDAARRMAQLETGLAD
                     VGSELERSETDVRAARGRMEAAIDKLAALEPQRASLELQRDDLRAQLAMARAVALTAQ
                     QLARELALQVESRRSSHGSLTSTLTRIEKQLEGLEARRDELAGQLVLGEAPLAAAQVE
                     LEQALEVRSAIEAELHAARIASEELDAHLRERDAGRVAVEQRVEAARSSLDDARFAAQ
                     QVRVRREGVAEQLAATSFELSALLAQLAPEAGVATWETLLEETRAKIERLGQVNLAAI
                     GEFKEQSERKEYLDRQCKDLTDALETLESAMRKIDRETRTRFQDTFDRVNSGLKEKFP
                     RLFGGGHAYLELTGEEGAAAGVSVMARPPGKRNSTISQLSGGEKALTAVALVFAIFDL
                     NPAPFCLLDEVDAPLDENNVGRFCDIVRDMSRNVQFIFITHNKSTMELASQLVGVTMN
                     EPGVSRLVTVDVDEAVRMAAM"
     gene            3969..4355
                     /gene="queF"
                     /locus_tag="VGE92_11090"
     CDS             3969..4355
                     /gene="queF"
                     /locus_tag="VGE92_11090"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEY1314416.1"
                     /translation="MTEPSTSLETFANPAPERDYTIRMTIPEFSCLCPKTGQPDFATL
                     KLEYVPHELCVELKSLKLYVWSFRNRGTFHEAVTNEMLGTLVSATGPRFMRLAAKFNV
                     RGGIYTTVVAEHRRPGWTGTAQVILP"
     gene            complement(4515..4703)
                     /locus_tag="VGE92_11095"
     CDS             complement(4515..4703)
                     /locus_tag="VGE92_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314417.1"
                     /translation="MAITGFDMGLLGFTITFDVTGDDLAFAGGFTTFFGAATLGVAFL
                     GVALPGRLAALLGFFEGI"
     gene            4693..5361
                     /gene="dksA"
                     /locus_tag="VGE92_11100"
     CDS             4693..5361
                     /gene="dksA"
                     /locus_tag="VGE92_11100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEY1314418.1"
                     /translation="MIAKPVIAKPAPAKPAAAKPVSVKPAVDKLPRVKPEAAAKVANE
                     KAPPIKFDTRPLVLTRVLPSDEDGDGETATSASLLAGPRNVKPYIARRGEQYMNKEQL
                     EHFARILQNWKRDLMVEVDRTVLHMKDEAANFPDPNDRATQEEEFSLELRTRDRERKL
                     IRKIDEALKRIEDGSYGYCLETGEEIGIKRLEARPVATLSVEAQERRERREKQYGDRD
                     DRYR"
     gene            5375..>6066
                     /gene="gluQRS"
                     /locus_tag="VGE92_11105"
     CDS             5375..>6066
                     /gene="gluQRS"
                     /locus_tag="VGE92_11105"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEY1314419.1"
                     /translation="MGRFAPTPSGDLHLGSLYTAAASYLDARSRGGRWLLRIEDLDRP
                     REAPGCADRILRTLEAFGFEWDGEIVRQGDRTDQYALALQRLQEHRLTFECSCSRLQL
                     EDESRYPGTCRERPSLLRVPTATRLRVEPGPILFTDRIQGAYRQDVAHAVGDVILQRR
                     DRVFSYLLAVVVDDAAQGVNHVVRGADLLDNTPRQIYLQRLLGLPLPAYAHVPVLTEV
                     DDTKLAKSRRSVR"
BASE COUNT         1062 a         2074 c         1846 g         1084 t
ORIGIN      
        1 gcggaggcaa acggtgctac ggccgcatcc gtgacggcca tggctgccgg cggcggcggg
       61 gcaacggggt ccaggtccac catcatcggc ccgtccaaga cgggcacgtg atcgaaatcg
      121 ccggttcgaa tattgagcgg ctcgaatggc ggaacgcccc actcgcgcga tgtcggctgc
      181 gcccgcatcc cctcatcgtt cggctcggta tagcgcggct cgagcggcac actcgactcg
      241 cgcagttcgg cattaccccg tgcttgactc gagcggcgcg cactccacca ccagatgccg
      301 gcgagcagcg gcacgctcag gcacagcaat atgaggcgca gctcggactg caattgcctg
      361 cgccctacat cgccgccatg cgcacggcct catcgacatc caccgtgacc aagcgcgaca
      421 ctccgggctc attcatggta actccgacca gctgcgatgc cagctccatg gtgctcttgt
      481 tgtgcgtgat gaagatgaac tgcacattgc gcgacatgtc gcgcacgata tcgcaaaagc
      541 ggccgacgtt gttctcatcg agcggcgcgt cgacctcgtc cagcagacag aacggcgccg
      601 gattcaaatc gaatattgca aacaccagcg ccaccgcagt caaggccttc tcgccgccgg
      661 acagctggct gatggtgctg ttgcgcttgc ccggcgggcg ggccatcacc gagacccccg
      721 ccgccgcacc ctcttcaccc gtgagttcca aataggcatg cccgccgccg aacaggcgcg
      781 gaaatttttc cttgagtccg gaatttaccc tgtcgaacgt atcctggaag cgtgtacgag
      841 tctcgcgatc gatcttgcgc atcgcggatt cgagggtttc cagggcatcc gtcaaatctt
      901 tgcactgccg gtccaggtat tccttgcgct cggactgctc cttgaattca ccgatggccg
      961 cgagattgac ctgccccaaa cgctcgatct tggcgcgagt ctcctcgagc agcgtctccc
     1021 aggtcgcgac gcccgcctcg ggcgcgagct gcgccaacag cgcgctcagc tcgaaactcg
     1081 ttgctgcgag ctgttctgcc acgccttcgc ggcgcacccg tacttgctgc gcagcgaagc
     1141 gcgcatcatc caggctcgag cgcgccgctt cgacgcgctg ctccaccgca acgcgtcccg
     1201 catcgcgctc gcgcagatgc gcgtccaatt cttcgctcgc gatccgcgcg gcgtgcaatt
     1261 cggcttcgat tgccgagcgg acttcgagag cctgctcgag ttcgacttgc gcggcggcaa
     1321 gcggcgcctc gcccagcacc aattgacccg ccagttcgtc gcggcgcgcc tccaagcctt
     1381 cgagctgctt ttcgatgcgc gtcaatgtgc tcgtcaaaga accgtgcgag gagcggcgcg
     1441 actccacttg cagcgccaat tcgcgggcca gttgttgcgc cgtcagagcc acggcgcgcg
     1501 ccatggccag ctgcgcgcgc agatcgtcgc gctgcaactc gaggctggcg cgctgcggtt
     1561 cgagcgccgc caatttgtcg atggcagcct ccatgcggcc gcgtgcggcg cgcacatccg
     1621 tctcgctgcg ctcgagttca gagcccacgt ccgcgagccc ggtctcgagt tgcgccatgc
     1681 gccgcgccgc atcgctgctg cgcgcctgcg cggaatcgaa ggccgcgcgg cgatcgacgt
     1741 gttcgcgatg caggcgattg acgttcgcct gaaactgttc gcgccggtct tcgtggtcgc
     1801 gcaggtgctc gcgagtgagc tcgagcttgc gctccaggcc cttgacctcg tccgcaagtg
     1861 cgccgacccg ggcatgcagc tcgcgcaagc tctcctcgcg ctcgatcacg ccggtatgcg
     1921 gatcggcatc gcgcgacagg cgcagccaat cctggcccaa ccagatgcca tcgcgcgtca
     1981 ccaccgattc cccggccgcc agccgcgcgc gctgccgcaa agcctcattc agagtctccg
     2041 cggtgtacac cgatgacaac accgaactca atacggtggc accctgcacc ttcgcctgca
     2101 gcgaagccgg ggcaagcggc gccgttctcg cctcggacat gctgaccacg gccaaatgag
     2161 cgccgtcgaa gctcgccaac aagtccgtca cgctgtccaa gccatcgacg catacggcct
     2221 cgaggtacga tcccaacacc gtttccacgg cacgctccca gccgcgctcg acgcgcagct
     2281 gctgtgcgac gcgcggctgg cggtcgaggg agttcgattt caaccactcg ctgaccttgc
     2341 cggagacctt gccgagtgcc gcttgctgca atgcctcggt cgatacctgg gcacctaagg
     2401 cttcctgcca gcggccgcgc aactcattga gcgccagcga ttgttcacgt tcccgatcgc
     2461 gcgagaccgc gatctccgcc agcaattcct gcagctcggt cgcggctttc tcgccggcgt
     2521 cgcgcgccaa ttgagcctgc gcttgcaagg tttccaaggc caccgggggc tgcaattgca
     2581 tgagctgcgc gcgctcgctc tcctggcgct cgcgctgctg cagcagacgc cgctgttcgc
     2641 cctccagctg ctcgatgcgc gcgcgttcgg ctccggtctc gccttggccg agcgccacgg
     2701 cctgcgcgtg cgcgtcccag gtttgctgcc aggccgcgag cgcgtgctcg caactcgcca
     2761 gcgcttccga ggtgcgcgct tcgtcggcgt gtgcggcgtc gaggccgggc accattgtcg
     2821 tgagttcctg cgagagctct tcgatctggc cgcgatcccg ttgcagcact tggccgagct
     2881 cggcaacctg cgcctcggct tgttccaagt cggcgcgctg gcgctggcgc agctcgcgcg
     2941 tgaactggat gctttgctcg atgcgcgtga cctcgccgcc cgcctcgtag aagcggctct
     3001 ggatggcgcc gagcgcatcg gccttgtgcg cctgatcggc gcgaatgcgc tcgatctcgg
     3061 cctcggctgc gcgcagatcg gcgagcacgc cttgcattgc ggtctcgcag ctcgaggtcg
     3121 cggcatcgcg cgttccggcc tccgcatcca ggctctgcag gcgcagcgcc aggagttcgg
     3181 cctgcagctt gcgttcttcc tctttcaacg cttgatagcg ccgtgcggtc gcggcttggc
     3241 gctgcaggtg gcggatttgc ttctcgactt cttcgcgcaa atcattgagc cgatccaggt
     3301 tctcacgcgt gtggccgatc cgattctcgg tctcgcgccg ccgctccttg tagcgcgaga
     3361 taccggcggc ctcttccaag aatgcacgca aatcgtccgg ccgcgcctcg atcacacgcg
     3421 aaatcatgcc ttgttcgatg atggcgtagc tgcgcgtccc caagcccgtg ccgagaaaca
     3481 actgggtaat gtctttgcgg cgcaccttgg tgccgttgat gtagtaatcc gaggtgccgt
     3541 cgcggctcac cgcgcggcgc agcgcaactt cgttgtaggc ggcatattcg ccgccgatct
     3601 tgccgtcgga gttgtcgaac acgagctcga ccgacgcggc acccaagggc ttgcgcgcgg
     3661 aggagccgtt gaagatcacg tccgccatgg attcgccgcg caaatgcttg gcggagattt
     3721 ctcccatcac ccagcgcacg gcatcgatga tgttggattt tccgcagccg ttcggaccca
     3781 cgacgcccgt caggctgctc ggaaaggaca cggtggtcgg atctacgaag gatttgaagc
     3841 cggcaatctt gagtttcgtt aagcgcatta tttcggtgaa cctagatgcg tggccgtcga
     3901 tgaggcgcgt agtgtaaagt aaacgtcact ttcgtaaacc cccgactgac tcgccgccaa
     3961 caccccgcat gactgaaccg agcacctcgt tggagacctt tgcaaatccc gcgccggaac
     4021 gcgactacac catccgcatg accatcccgg agtttagctg cctgtgcccg aaaacgggac
     4081 agccggattt cgcgacgctc aagctcgaat acgttccgca cgagctttgc gtcgagctca
     4141 aatcgttgaa gctttacgtc tggtcgttcc gcaaccgggg cactttccac gaagcggtca
     4201 ccaacgaaat gctgggcacg ctggtgagcg caaccgggcc gagattcatg cgcctagcgg
     4261 ccaaatttaa cgttcgcggc ggcatttaca cgacggtcgt agccgaacat cgccgccccg
     4321 ggtggacggg aaccgcccag gtgatactcc cgtaaagccc gccgccgcgg ctcgcccgcg
     4381 ccttgcccga cccctacccg ggggttttgc ttcccaatca taggcttaag tataaaaata
     4441 tgttgctcca gtgccctgta caggccgctt tgcgtcggta taatcgcgcg ctctttcgtt
     4501 aagaggcgga gacttcaaat gccctcgaag aaacccaata aagcggctaa gcggccaggt
     4561 aaggcgacac ccaagaaagc gactcccaag gtcgccgccc cgaaaaaggt ggtaaagccg
     4621 cctgcgaaag ccaagtcatc gcccgtcaca tcgaaggtaa tagtgaaacc caataagccc
     4681 atatcaaaac ccgtgatcgc caagccagtg atcgccaagc ccgcgcccgc gaagccggcg
     4741 gccgcaaagc ctgtctccgt caaacccgcg gttgacaagc tgccgcgcgt gaagcccgag
     4801 gccgctgcga aagtcgccaa tgaaaaggcg ccgcccatca aattcgatac gcgtccgctg
     4861 gtgttgacgc gtgtactgcc gagcgatgag gacggcgacg gcgaaaccgc cacctccgcc
     4921 agtctgcttg cgggcccgcg caatgtgaaa ccgtacatcg cccgccgcgg cgagcagtac
     4981 atgaacaagg agcagttgga gcacttcgcg cgcattctgc agaactggaa gcgagacctc
     5041 atggtcgagg tggatcgcac ggtgctgcac atgaaggacg aagccgccaa ttttcccgat
     5101 cccaacgacc gcgcgacgca ggaagaggaa ttcagcttgg agctgcgcac ccgcgaccgc
     5161 gaacgcaagc tcatccgcaa gatcgacgaa gccctgaagc gcatcgaaga tggttcatac
     5221 ggctactgtc tcgaaacggg cgaagagatc ggcatcaagc gattggaggc gcggccggtc
     5281 gccaccttgt cggttgaggc ccaagagcgg cgcgagcgcc gcgaaaagca gtacggcgac
     5341 cgcgacgacc gctaccgcta aaacgcagcc ctacgtgggc cggttcgcac cgacgccctc
     5401 gggcgacctg cacttgggtt cgctgtacac ggcggcggcc agctacctcg atgcccggtc
     5461 gcggggcggt cgctggctgc tgcgcatcga ggacctcgac cggccgcgcg aggccccggg
     5521 ttgcgccgac cgaatcctgc gcaccctcga ggccttcggt ttcgaatggg acggtgagat
     5581 cgtgcgccag ggcgatcgaa ccgaccagta cgcgctcgcc ctgcaacgat tgcaggagca
     5641 ccggctgacc ttcgagtgct catgcagccg cctgcagctc gaggacgaga gccgttaccc
     5701 cggcacctgc cgcgagcggc cgtcgctgtt gcgggtgccg acggccacgc ggctgcgggt
     5761 cgagcccggc ccgatcttgt tcaccgatcg catccaaggg gcctaccgtc aagacgtcgc
     5821 tcacgctgtc ggcgacgtca ttctgcagcg gcgcgaccgg gttttctcct acctcttggc
     5881 ggtggtggtc gacgatgccg cccagggggt gaaccatgtg gttcgcggcg ccgacctatt
     5941 ggacaacacg ccacggcaaa tctacctgca gcgcctgttg ggattgccgc tgccggccta
     6001 tgcgcacgtc cccgtgctca ccgaggtcga tgacaccaaa ctcgcgaagt cgcgacgcag
     6061 tgtgcg
//
LOCUS       DASWZT010000394         3488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_46721, whole genome shotgun sequence.
ACCESSION   DASWZT010000394 DASWZT010000000
VERSION     DASWZT010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46721"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VGE92_11110"
     CDS             complement(<1..253)
                     /locus_tag="VGE92_11110"
                     /inference="COORDINATES: protein motif:HMM:NF019625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HEY1314420.1"
                     /translation="MKTMLDLVGRHKSGAPVGVYSVCSAHPMVIEAALRLARRTGGVA
                     LVEATSNQVNQDGGYTGMRPAMFHERVQALANAVGLPHER"
     gene            complement(250..1152)
                     /locus_tag="VGE92_11115"
     CDS             complement(250..1152)
                     /locus_tag="VGE92_11115"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY1314421.1"
                     /translation="MNKPWLTYALITTGFWGVWGAFAEFPTRHGFPETLVYVVWALTM
                     IPPALYAMHRTGWRVQRDAKSILLGLLIGLTGAGGQMLLFHAVHTGPTYLIFPIIALS
                     PAITIAMSMVFLKERVTKVGALGAALALIALPLFNYSPGGDAAATGLEGVTWFIYALI
                     ILLAWGVQAYFMKLANASMNAENIFLYMTVTGLALVPVALWMTDFRQPIEYGWQGPGL
                     AAITQILNAVGALTLVYAFRYGRALVVAPLANAGAPMITAIVSMIVLGVMPNAITVLA
                     IALAVLAAALLALEPEEKLIEQPV"
     gene            complement(1154..>3488)
                     /locus_tag="VGE92_11120"
     CDS             complement(1154..>3488)
                     /locus_tag="VGE92_11120"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY1314422.1"
                     /translation="TRTDALNAYTQDIYDATAFPFEDSNMTPPAGTPLAAAPCCITFG
                     SIFDEKKREALSGSLEWRPNDALKLVADGLWTKLQDPQIGYNQSYYFPYGVDQNGNGT
                     WSDSSATIKNGVVTGVTANNFTPEIVNNTTDRNVVTSMYGLKGTWTPTDKFSLGFDAY
                     RSTANRPEGGTDTFVTAGLVSALPNAEDYITLTDLPHSLPSLNVVIPPSQLGLSACPS
                     GTSSATRAGYCSYTALMNSGFLDNNKYWSTHYDGLNGYSVHDQVTGFTLDGDYKASLG
                     FFDKLLFGVGYNHREKSRVDVSNDWDNGSGQYGTLYQTAGCPVQCSPYSFGSQGFNVI
                     SFTSPPNFMQGAGGSYPLVLPQLNTAQLLAFLASLNGKPNPTFCTSLPCATPFNLAST
                     LPQPNPFNSYRVAEKTYSFYTEATFAGNSWSGNVGVRVVRTTTVAATAESVPTDIWTN
                     NATNSVQSFNVDYSTAAQFLQEATYTLALPSANLSYWVVPEQLQFRAAAGETMSRPNL
                     NQLAPNSTNQAINGTPEIDYTGTAGLKPIKAWSVDLSLEWYYQPHAALNAALFGKKVS
                     NDIYTATQTSVNLGTLEYDNGPPGSPNNPSKPFLWTITAPANGDKSTYTGIELSWQHF
                     LENGLGTHMQYTHTWSKGYDQFGNPTGAVNAAPPTTFSVSLIYDKGPFNADVNWDYTS
                     SYTTYCSACTEVPGWPAIADSFSWVTASAHYRIYKGFEVYVEGKNLTNAIARTYLNGN
                     PLLPWGPGQLIGESASGTGQGYSAYGRSYVLGASYRF"
BASE COUNT          645 a         1038 c         1133 g          672 t
ORIGIN      
        1 cccgctcgtg cggcaggccg acggcgttcg cgagtgcctg gacccgctcg tgaaacatcg
       61 cgggccgcat gccggtgtag ccgccatcct ggttcacttg attcgaggtg gcttcgacca
      121 acgcaacacc gccggtgcgg cgcgcaagcc gcagcgcggc ctcgatcacc atgggatgcg
      181 cggagcacac ggagtagacg cccaccgggg cgccggactt gtgccggccg accaaatcca
      241 acatcgtctt catacgggtt gctcgatgag cttctcttcc ggttcgaggg cgagaagcgc
      301 tgcggcgagc acggccagtg cgatggcgag cacggtgatc gcgttcggca tgacgccgag
      361 cacgatcatc gagacgatcg ccgtgatcat cggcgcgccg gcattggcca gcggcgcgac
      421 caccagcgcc cggccgtagc gaaaggcgta aaccagcgtc agggcaccca ccgcgttcaa
      481 gatctgcgtg atcgcggcga gccccggccc ctgccaccca tattcgatcg gttgcctgaa
      541 gtcggtcatc cacagtgcca ccggaaccag ggcgaggccc gtgacggtca tatacaaaaa
      601 aatattctcg gcattcatgg aggcattggc gagcttcatg aaataggcct gcacgcccca
      661 ggcaagcaga atgatcaacg cgtagatgaa ccacgtcacg ccctcaagtc cggttgctgc
      721 cgcgtcgccg cccggggagt aattgaacag cggcagcgcg atcagggcca gcgccgcgcc
      781 aagcgcgccc accttcgtca cccgttcctt gagaaacacc atcgacatcg caatggtgat
      841 cgcgggcgac aaggcgatga ttggaaagat caggtacgtg ggcccggtgt gcacggcgtg
      901 gaacagcagc atctgtccgc ccgcccccgt caatccgatc agcagcccca agagaatcga
      961 cttcgcgtcc cgctgcaccc gccatcccgt gcgatgcatg gcgtacagcg ccggtggaat
     1021 catcgtcagc gcccacacca cgtacaccag cgtttcggga aagccgtggc gcgtcggaaa
     1081 ttcggcgaat gcgccccaca ccccccagaa cccggtggtg atcagcgcat acgtgagcca
     1141 gggtttgttc acgctaaaat cggtacgagg cgccgagcac gtagctgcgt ccgtacgcgc
     1201 tgtagccctg gcccgtgccg ctggcgctct cgccgatgag ctggcccggg ccccacggca
     1261 acagcggatt gccgttgagg taagtgcgcg cgatggcatt ggtgagattc ttgccttcga
     1321 cgtacacctc gaagcctttg tagatgcggt agtgagcgct tgccgtgacc cacgagaagg
     1381 aatcggcgat ggcgggccaa cccggcactt ccgtgcatgc cgagcaatag gtggtgtagc
     1441 tggaggtgta gtcccagttc acgtccgcgt tgaacggacc cttgtcatag atcaagctga
     1501 ccgagaacgt ggtgggcggc gctgcgttga ccgcgccggt gggattgccg aactgatcgt
     1561 agcccttgct ccaggtatgg gtgtactgca tgtgcgtgcc caagccgttc tcgaggaagt
     1621 gctgccagct caactcgatt ccggtgtagg tcgacttgtc gccgttggcc ggagccgtga
     1681 tggtccagag aaacggcttc gacgggttgt tcggtgagcc cggcggtccg ttgtcgtatt
     1741 ccagagtgcc tagattgacg ctggtttgcg tcgcggtgta gatgtcattg gagactttct
     1801 tgccgaacag cgccgcgttg agcgccgcgt gcggctggta ataccattcg agcgacagat
     1861 cgacggacca ggccttgatg ggcttcaagc ccgcggtgcc ggtgtagtcg atctccggcg
     1921 tgccgttgat tgcttggttg gtggagttcg gcgccagctg attcaagttc ggacgcgaca
     1981 tggtctctcc cgccgccgcg cgaaactgca gctgctcggg cacgacccaa tagcttaagt
     2041 tggcggaggg cagcgccaga gtgtaggtcg cttcctgcag gaattgcgct gcggtgctgt
     2101 aatccacatt gaagctctgc accgagttgg tcgcattatt ggtccatata tccgtcggga
     2161 cggactcggc ggtggcggcc acggtcgttg tgcgcacgac acgcacgccg acgttccccg
     2221 accagctgtt accggcgaag gtcgcttccg tgtagaacga ataggtcttc tcggcgactc
     2281 gataggaatt gaacgggttc ggctgcggca acgtcgaggc gaggttgaaa ggcgtggcgc
     2341 acggcagcga cgtgcagaag gttggattgg gtttgccgtt caaactcgcc aggaacgcga
     2401 gtagctgggc ggtattcagc tgtggcagca ccaacggata cgatccgccc gcgccctgca
     2461 tgaaattcgg cggactcgtg aacgaaatga cattgaatcc ctgtgagccg aaggaatagg
     2521 gactgcactg caccggacat cctgcggttt ggtacagcgt gccgtattga ccggacccgt
     2581 tgtcccagtc gttgctgacg tcgactcggc tcttttcgcg atgattgtag cccacgccga
     2641 acagcagctt gtcgaagaat cccagactcg ctttgtagtc gccgtcgagc gtgaagccag
     2701 tgacctggtc gtgcacggaa tagccgttga gtccatcgta atgggtggac caatatttgt
     2761 tgttgtccag aaacccggag ttcatcaacg ccgtgtaaga acagtagccc gccctggtgg
     2821 cgctcgaggt tccgctcggg caggccgaca ggccgagctg gctcggcgga atcaccacgt
     2881 tgaggctcgg caggctgtgc ggcaggtcgg tgagcgtgat gtagtcctcg gcgttcggca
     2941 acgccgacac caggcccgcg gtcacgaacg tgtcggtgcc gccctcgggc cgattcgccg
     3001 tcgaacgata ggcatcgaag ccgagcgaga acttgtcggt gggcgtccac gtgcccttca
     3061 agccgtacat cgaggtgacg acgttgcggt cggtggtatt gttgacgatc tccggcgtga
     3121 agttgtttgc cgtcactccg gtcaccaccc cgtttttaat ggtcgcgctc gaatcggacc
     3181 aggtgccgtt gccattctga tcgacgccgt acgggaagta atacgattgg ttgtagccga
     3241 tctgcggatc ctgaagcttg gtccagaggc cgtcggcgac cagcttgagc gcgtcgttcg
     3301 gccgccattc gagactgccg gacaaggctt cgcgtttctt ctcgtcgaag atcgagccga
     3361 acgtgataca gcagggcgcg gccgcaagcg gcgtccccgc gggcggcgtc atgttcgaat
     3421 cctcgaaggg gaaggcggtc gcatcgtaga tgtcctgggt gtaggcgttc agcgcatcgg
     3481 tgcgcgtg
//
LOCUS       DASWZT010000395         3195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_51388, whole genome shotgun sequence.
ACCESSION   DASWZT010000395 DASWZT010000000
VERSION     DASWZT010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51388"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..1976)
                     /locus_tag="VGE92_11125"
     CDS             complement(171..1976)
                     /locus_tag="VGE92_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HEY1314423.1"
                     /translation="MKPFEPRALFGALAAIAALALFCDADALPPAAYRPIVPSMADQT
                     VVLTGRDLTIEQVVQVARYGAKVQLSPEARQRSADAHALLLEAAAEGISVYWFNRGSG
                     SGRERFIFTGDPLAPENKKLLEERQLAIFKRGAWAGLGPEINDEEIVRAMLVVRANTM
                     SFEAASPQLTQRLLELLNERISPVVQSRGTVGEGDLGPFTNVAATMVGAGNAYFRGVR
                     MSAAAALQAAGLAPLAPFGADDSELESSNAYATGQTALLLHDAREALSWADLIYALDL
                     NGMNSSISPLSQPVQANRPFKWLNWDAARVLDMLRGSYLFELDDKRIIQDPESLRASA
                     IRQGSAWQAWAQLRDVALIQMNSSDHNPAVRVGASPGDSWELSTPQLAQFYVKGSARN
                     HGQHGFIFSNANWDPYPIANEIEAFTIALANMDTAVAQRVIRFTSPFFTVASPSDESP
                     GEPGRFHPTQGSEIDVVALMQEIQGLINPVPPEGNALIQNVEDMQTQTRLKVTRARAA
                     VEDTVELLAEDLLTGTYWLDQRKSQNAAVSFGASPTAVWTAFRATLPFNGAGPATATQ
                     PIHEIAAAFIRSHAAINFYPDNAREPDAMTRRHIE"
     gene            complement(1983..>3195)
                     /locus_tag="VGE92_11130"
     CDS             complement(1983..>3195)
                     /locus_tag="VGE92_11130"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY1314424.1"
                     /translation="RELIADAPGAMQAGELHLSIGHCFKALGHQPEAIAAYREAAAAH
                     PSFGDAYWSLANLKTYRFSDAEIARMRAQQAAAATSEVDRYHLCFALGKALEDRGEYA
                     ESFGFYARGNTLKRAETGYRAEPVERNTRLQKAICTRELFAARQGVGSDDSSPIFIVG
                     LPRSGSTLLEQILASHSQVEGTMELATIPRLVLELQGREPDSENPRYPKVLAELKPAE
                     FRKLGEKYLAEAGSYRTGKPRFIDKMPNNFRHLGLIHLILPNAKIIDARRNAMACCFS
                     NFKQLFAAGQDFTYGLEEIGRYYRTYVELMSHWESVLPGKVLRVEHEAVVEDLETSVR
                     RILEFCGLEFEPQCVEFYKTERSIRTASSEQVRRPIFKEGLDQWRHFEPWLGPLKEVL
                     GPLAGSASRQP"
BASE COUNT          577 a          971 c         1023 g          624 t
ORIGIN      
        1 catgaagacg cagcccaagt cccggcgccg ccaaagcttg tcgatggctt cgggtgcgcg
       61 atgctcgagc agatgcatgt cgaggccgct ccgctcgatg agtgcagcaa gcagccgtcg
      121 ccaaagctcg gccgcctctg ggctaaccga gtacatcctg gcgttggcga tcattcaata
      181 tgccggcggg tcatcgcatc gggttcgcgc gcattgtccg ggtagaaatt gatcgcagca
      241 tggctgcgaa taaaagctgc tgcaatttcg tggatgggct gggtggccgt ggccggcccg
      301 gccccgttga acggcagcgt ggcgcgaaac gccgtccaaa cggccgtcgg tgaggcgccg
      361 aaactcacgg ccgcgttttg cgacttgcgc tggtccagcc agtacgttcc ggtcagaaga
      421 tcttccgcca agagctcaac ggtatcctcg accgcggcgc gcgcgcgcgt aaccttgagc
      481 cgcgtttgcg tttgcatgtc ctcgacattc tgaatgagag cattgccttc gggcgggacg
      541 gggttgatga gcccttggat ttcctgcatc aaggccacta catcgatttc actgccctgg
      601 gtcggatgga agcgtcctgg ttcgcccggc gactcgtcgg aaggagacgc gaccgtgaaa
      661 aaaggactgg tgaagcggat cacccgttgc gccaccgccg tgtccatgtt ggcgagcgcg
      721 atggtgaagg cttcgatttc attcgcgatc gggtacggat cccaattcgc gttcgagaat
      781 atgaaaccgt gctggccgtg attgcgcgcg ctgcctttga catagaactg cgccaattgc
      841 ggggtgctca gttcccaaga atcgccgggc gaagcgccga cgcgaaccgc cggattgtga
      901 tccgaagagt tcatttggat cagggccaca tcgcgcaatt gtgcccacgc ctgccaggcg
      961 gatccctgac ggatcgccga ggcgcgcaga ctctcggggt cctggataat cctcttgtcg
     1021 tcgagctcga acaggtagct gccacgcagc atgtcgagca cgcgcgccgc atcccaattc
     1081 agccacttga aaggtcgatt ggcctgcacc ggctgcgaca ggggtgagat gctgctgttc
     1141 atgccattga ggtccagcgc gtagatcaga tccgcccagg acagcgcttc gcgggcatcg
     1201 tgcagaagca acgccgtttg ccccgtcgcg taggcgttgc tgctttccaa ttcactgtcg
     1261 tccgcgccga acggcgcgag cggcgcgagg cctgccgctt gcagcgccgc cgcggccgac
     1321 atgcgcacgc cgcggaaata cgcattgccg gcgccgacca tggtcgcagc gacgttggtg
     1381 aagggtccga gatcgccttc gccgacggta cctcgagact ggaccaccgg gctgatgcgc
     1441 tcattcagga gctcgagcag ccgctgcgtc agttgtggac tggcggcttc gaaactcatg
     1501 gtgttggcgc gcaccaccag catggcgcgg acgatttctt cgtcgttgat ttcggggccg
     1561 agtcccgccc acgcgccgcg cttgaagatg gcgagttgcc gctcttccag aagtttcttg
     1621 ttctcgggcg caagcggatc gccggtaaag atgaatcgtt cgcggccgga acccgagccg
     1681 cgattgaacc aatagaccga aatgccctcg gccgcggctt cgagcagcaa ggcatgcgca
     1741 tcggcggagc gctgccgcgc ttccggactc aattgcacct tcgcgccgta gcgcgccact
     1801 tgcacgactt gctcgatggt taaatcccgt ccggtgagca ccacggtctg gtcggccatg
     1861 ctcgggacaa tggggcgata ggcagctggg ggtaaggcgt cggcatcgca gaacaaggcg
     1921 agcgccgcga ttgcggcaag agcgccgaac agggcacgtg gttcaaacgg cttcatggct
     1981 acctagggtt gtcgactcgc gctgcccgcc aaaggcccga gcacctcttt caatggtccg
     2041 agccagggtt cgaaatgccg ccactggtcg aggccttctt tgaatatggg ccggcggacc
     2101 tgctcggaac ttgcggttct gatgctgcgc tcggtcttgt agaattccac gcactgcggc
     2161 tcgaattcaa gaccgcagaa ttcgagaatg cgacgaacgc tggtctcgag atcctcgacc
     2221 accgcttcgt gctcgacccg cagcactttc cccggcagca ccgactccca atgactcatg
     2281 agttcaacgt aggttcgata gtaccggccg atctcttcga gtccgtaggt gaagtcctgg
     2341 cctgctgcga acaattgctt gaagttgctg aaacagcagg ccatggcatt gcgtcgcgca
     2401 tcgatgattt tggcgttggg caaaatcagg tgaatcaggc ccagatggcg gaagttgttc
     2461 ggcatcttgt cgatgaagcg cggcttgccg gtgcgatagc tgccggcctc ggccaggtat
     2521 ttctcgccca acttgcgaaa ttccgcaggc ttgagctcgg cgagcacctt cgggtaacgc
     2581 gggttctcgc tgtccggctc acggccctgc agttcgagca ccaggcgcgg gatggtggca
     2641 agttccatgg tcccttcgac ttgcgagtgc gaggcgagta tttgttccaa caaggtcgag
     2701 ccggaacgcg gcaaaccgac gatgaatatg ggcgaggaat cgtcggatcc gacgccctgc
     2761 cgcgcggcaa atagttcgcg cgtgcaaatc gccttctgca ggcgggtgtt gcgctcgacc
     2821 ggctccgcgc ggtagccggt ctccgctctc ttcagcgtgt tgccgcgagc gtaaaagccg
     2881 aacgattcgg catattcgcc gcggtcttcg agtgccttgc cgagcgcgaa gcacaggtgg
     2941 tagcggtcga cctcgctggt cgccgccgcc gcctgttggg cgcgcatccg tgcaatctca
     3001 gcgtccgaga agcgataggt cttgagattg gcgaggctcc aatacgcatc gccgaagctt
     3061 ggatgagccg ccgcggcctc gcggtaggcc gcaatcgcct ccggctggtg ccctaaggcc
     3121 ttgaagcaat ggccgatcga caggtgcagc tcgccggctt gcatcgcccc gggcgcatcg
     3181 gcgataagtt cgcgg
//
LOCUS       DASWZT010000396         5603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_90128, whole genome shotgun sequence.
ACCESSION   DASWZT010000396 DASWZT010000000
VERSION     DASWZT010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90128"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..2509)
                     /locus_tag="VGE92_11135"
     CDS             complement(23..2509)
                     /locus_tag="VGE92_11135"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEY1314425.1"
                     /translation="MNVSRITGLLILTASIALSACGGGGAPTTTNEASAPTSSADAYT
                     GPAPATADVQAFAVNFWANVRTQNRCGQCHNATSPAQMPNFARSDDVNLAYAQANTVV
                     NLASPATSLIVTKVSGGHNCWLADASACGQILTTWISNWAGATGAASATQVQLVAPPI
                     QSVGQSLVFPASYTEYQNTVYTLTSKYCVRCHSSTASAATMQSPYFADPTLAIAYPAA
                     IPKIDLTGCSPFVAGNGAGACGTNSRFYQRLLTDNHNCWDNCATDAAQMLTEIQAFAK
                     SLTPTSLDPNLVVSKAITLAQGTVASGANRYDADTIAKYEFQTGSGYTAYDTSGIDPA
                     ADLTISGNVLWAGGWGINIGAGGKAQASTSSSSKLYTLIQATGEFSVEAWVAPALVAV
                     DNSYMVSYSGGDTLRNFTLGQTNQDYDFMLRSSNSVLNGTPQLKTPDAALLLQASLQH
                     VVLTYDPVNGRQIYVNGVNANVPDPQKGGTISNWDNTFALVLGNEVSGDRSWQGLIKF
                     VAIHSRALSAAQVMQNFNAGVGQHYYMLFNVAEVTGVSQAYVMFTVSQYDSYSYLFYQ
                     PTFISLDPTVKPNNIPVKGIRIGLNGNIPQVGQAYIPLNTTVTAAGYTPTGEVLSTVG
                     TVIGLQSGPLTDQFFLTFDQLGSHTNVVVEPTPVTPPPSFGPVVADIGVRTFAQVNAT
                     LSQLTGVPTSNAAVAATYLAVQQQLPADPTLEGFSSSNQVGIAQLAIQYCNVMVNTPA
                     YAAVVLPGVTINASTYPANTSQVTNALAARVLGSGLNSQPASSTVTTELGNLINTLCS
                     TSACTTAARAQAVTAAACATAFGSADMLIN"
     gene            complement(2512..3726)
                     /locus_tag="VGE92_11140"
     CDS             complement(2512..3726)
                     /locus_tag="VGE92_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314426.1"
                     /translation="MVKAESVGRGGSRRCVRTSALAGALAMLSATTFAAGPNADQATR
                     MYNRIAGIPPTAAELASMTGAADAVTAALIATQDPSFYNNTIRNLAAPWTNRDQSVFV
                     PLNDYTATVVGMVRDDVPFNTLLTADLLYIGDSASGAPAYSPANNDMYTTMDTNNVDL
                     KAHLTATTQSALTGIPSAATAGVMTTRGAASAFFVNGTNRAMFRFTVMNHLCNDLPTV
                     MDITRPPDRIRQDVTRSPGGVSTLFLNNCIGCHSGMDPMAQAFAYYNFSYPASEVGAT
                     AWPIGQIVYTAGQTQPKYHINNTNFPQGFNTPDDSWSNRWRAGPNEILGWSASLPGNG
                     NGAKSLGLELESSAAFASCQVTRVFKDVCLRAPNSSADFAQVTSMIASFQAHQYSLRQ
                     VFAEGAAYCMGN"
     gene            4072..>5603
                     /locus_tag="VGE92_11145"
     CDS             4072..>5603
                     /locus_tag="VGE92_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314427.1"
                     /translation="MNTLPTRELRHACMKLAALLLPALLMVGCESGVSGHAPGLAGLQ
                     TLDPGTLNFPMAYVKRPAPANTPNADIDVRDLITSTTGGDLYIRQQASVGSVETNITK
                     SITMGMGDVRDLDVSADGKKLVFSLRLPLNPNKPNDDITQPNWKIYEYDAVAQTVTQL
                     TNDNTTAGHDVGAHYLPDGRIVFASTRQAATQAILIDEGRPQYPAQTDDRKQPIFLLH
                     VMNADGSNIHQISMNTNHDFAPSVLANGQIVFSRYESINGDQISLYRSNPDGTGLELY
                     YGENSHATGANIAGTNNNVIQFLNARQRADGQLIAIVRPFLGTQQGGDIVQINAENFV
                     EIHQPATPGGAAGTAQSSATTLGVTTDANMPSLGGRFASAYPLYDGTNRMLVSWAPCL
                     IIDTTVTPNATKVCTAANTSGANVQLAPPQYTVWIYDVSAGTLSPILGAEINTVIVEP
                     VIMQARTPVPAFVPDVVPSGAAANLANNTNGGLGILEIRSVYDFDGVDEVSAETAGSI
                     PN"
BASE COUNT         1047 a         1725 c         1771 g         1060 t
ORIGIN      
        1 agtcctcttc gattcatcgt gcttaattga tcaacatgtc ggcgctgccg aaggcagtcg
       61 cacacgctgc ggccgtgact gcctgcgcac gtgccgcggt ggtgcaggcc gaggtgctgc
      121 acagtgtgtt tatcaagtta ccgagctcgg tggtcacggt ggaagacgcc ggctgactgt
      181 tgagcccgct gcccaaaacc cgcgccgcaa gtgcattggt cacttgcgat gtgttggccg
      241 gataggtcga ggcattgatc gtcacgcccg gcaggaccac cgcggcgtag gccggcgtat
      301 tcaccatcac attgcagtat tggatcgcca actgcgcaat gcccacctgg ttcgaagacg
      361 agaagccctc gagcgtcgga tcggccggca gctgctgctg cacggccagg taggtcgcgg
      421 cgacagcggc gttcgacgtc ggaacacccg tgagttgcga cagcgtggca ttgacctgcg
      481 cgaacgtgcg cacgccgatg tcggcaacca ccggaccgaa cgacggcggc ggcgttaccg
      541 gtgtcggctc gaccaccaca ttggtgtggc tgccgagttg atcgaaggtg aggaagaact
      601 gatcggtcag aggcccgctc tgcagcccga tcacggtacc gacggtcgac agcacctcac
      661 ccgttggcgt atagcccgcc gcggtcaccg tcgtgttgag cggtatgtag gcctggccga
      721 cttgcggaat gttgccgttc aagccgatgc gaatcccctt gaccgggatg ttgttcggtt
      781 tgacggtcgg atcgagcgaa atgaacgtcg gctggtagaa caagtagctg tagctgtcgt
      841 actggctcac cgtgaacatc acgtaggcct gcgatacgcc ggtcacttcc gcgacattga
      901 acagcatgta gtagtgctgg ccgacgccgg cgttgaagtt ctgcatcacc tgggctgccg
      961 acaacgcgcg gctgtggatg gcgacgaact tgatcaggcc ctgccatgag cggtcgccgg
     1021 acacttcgtt gccgagcacc aaggcaaagg tgttgtccca gttggagatg gtgccgccct
     1081 tttgcggatc cggaacattg gcgttcacgc cgttgacgta gatttgacgg ccgttgaccg
     1141 gatcgtaggt caggaccaca tgctgcaagg aagcctgcag cagcagcgcc gcatcgggcg
     1201 tcttgagctg cggcgtgccg ttcaggaccg aattcgaact acgcaacatg aagtcgtagt
     1261 cttgattggt ctgaccgagc gtgaagttgc gcagcgtgtc gccgcccgag tagctgacca
     1321 tgtaagagtt gtcgacggcc accagcgccg gcgccaccca ggcctcgacc gagaactcgc
     1381 cggtcgcctg gatcaaggtg tacaacttcg agctcgagga ggtgctcgcc tgcgccttgc
     1441 cgcctgcgcc gatattgatg ccccagccgc cggcccagag cacattgccc gagatggtga
     1501 gatcggccgc gggatcgatg ccgctggtgt cgtaggcggt gtagccgctg ccggtctgga
     1561 actcgtactt cgcaatggta tcggcgtcgt agcgattggc gccgctcgcc accgtaccct
     1621 gcgccagggt gatcgccttg ctgacgacca ggttcggatc gagactggtc ggcgtcagcg
     1681 atttcgcgaa cgcctggatc tcggtcagca tctgtgccgc atcggtggcg caattgtccc
     1741 aacaattgtg gttgtcggtc agcagacgct gatagaagcg cgaattcgtg ccgcacgcgc
     1801 ccgcaccgtt gccggcgacg aacgggctgc agcccgtcaa gtcgattttc ggaattgccg
     1861 ccggataggc gatcgccaac gtcgggtcgg caaagtatgg cgactgcatc gtggcggcac
     1921 tcgccgtcga ggagtggcag cgcacgcaat acttgctggt gagcgtgtac accgtgttct
     1981 ggtattcggt gtacgaggcg gggaagacca gactctggcc gactgattga atcggcggcg
     2041 ccaccagctg cacctgcgtg gccgacgccg cgccggtcgc accggcccag ttggagatcc
     2101 acgtggtcaa gatttgtccg caagcgctcg cgtcggcgag ccagcagtta tgtccgccgc
     2161 tcaccttggt cacgatcagc gaagtggcgg gcgaggcgag attgacgacg gtgttggcct
     2221 gcgcataggc caggttcaca tcgtcgctgc gcgcgaagtt cggcatctgt gcggggctgg
     2281 tcgcattatg gcactggccg cagcgattct gcgtgcgcac gttggcccag aagttgaccg
     2341 caaaggcctg cacatccgca gttgcgggcg cgggacccgt gtaggcatcc gccgagctgg
     2401 tgggcgcgct cgcctcgttg gtggtggtcg gcgcgccgcc gccgccgcag gccgagagcg
     2461 cgatcgaggc cgtgagaatg agcaggccgg taatgcgtga aacgttcatg ctcaattccc
     2521 catgcagtag gcggcgcctt cagcgaagac ctgccgcaat gaatattgat gcgcttggaa
     2581 tgaggcgatc atcgaggtga cctgtgcgaa gtccgccgaa ctgttcggcg cacgcaggca
     2641 cacatccttg aacaccctcg tgacctggca gctggcgaag gccgcgctgc tctcgagctc
     2701 gagtccaagc gatttcgcgc cgttgccgtt gcccggaaga ctcgcgctcc atcccaagat
     2761 ctcgttcggg ccggcgcgcc agcggttgct ccagctgtcg tccggtgtgt tgaagccttg
     2821 cgggaaattg gtgttgttga tgtggtactt gggctgcgtc tggccggcgg tgtagacgat
     2881 ctgtccgatc ggccacgccg tcgcaccgac ttcgctcgcc gggtagctga agttgtagta
     2941 ggcgaaggcc tgcgccatcg gatccatgcc gctgtggcag ccgatgcagt tgttcaggaa
     3001 caaggtgctc acgccgcccg ggctcctggt cacgtcctgg cggatgcgat cgggcggccg
     3061 cgtgatgtcc atgacggtcg gcaagtcgtt gcacagatga ttcatgaccg tgaagcggaa
     3121 catcgcgcga ttcgtgccgt tgacgaagaa cgcgctggcg gcgccgcgcg tggtcatcac
     3181 accggcggtg gccgccgacg gaatgccggt gagcgccgac tgcgtggtcg ccgtcaagtg
     3241 cgccttcagg tcgacattgt tggtatccat cgtggtgtac atgtcgttgt tcgccggcga
     3301 atacgccggt gcgcccgacg ccgagtcgcc gatgtacagg agatccgccg tcagcagcgt
     3361 gttgaacggc acgtcgtcgc gcaccatgcc gaccaccgtc gcggtgtagt cgttgagagg
     3421 cacgaacacc gactgatcgc ggttggtcca tggcgccgcc aggttgcgga tggtgttgtt
     3481 atagaaagaa gggtcttgcg tcgcgatcaa cgcagccgtc accgcatccg ccgcgccggt
     3541 catcgaggcg agctctgcgg ccgtcggcgg gatgccggcg atgcggttgt acattcgcgt
     3601 cgcctggtcg gcgttcggtc ccgcggcgaa agtggttgcg ctcagcatgg cgagtgcgcc
     3661 ggcgagcgcg ctcgtgcgca cacatcgccg cgaaccgcct ctgccgaccg actctgcctt
     3721 aaccatcccg ttcttcatat gcacatccac tctcgtgagt tgtgatttcg acgtggcgcg
     3781 aatatacgca gtagaaaagc cttcaagtaa tgtctcatcc gaacgacgtc ggtgtttcgc
     3841 gacatatcga ctgacccccc tccgcctata tccttcaggc gcaatttgcg aaggtctgcc
     3901 ttgatatgca cagagaaacg gaagtgacga agttcttctg tgagtgcgtt aacagtatgc
     3961 acacgcacat cgaacgaaga tgcggttctg aaaccgatta agttcaatga gctcgcgcag
     4021 aaacccaaac cgatccgtga acgaaattat tgaacgtagg tatctcgaat catgaacact
     4081 ctgccgactc gtgaactccg tcacgcttgc atgaaactgg cggcgctgct gctgcccgcg
     4141 ttgctcatgg tcggctgcga gagcggcgtg agcggtcatg cgccgggttt ggccggtttg
     4201 caaaccttgg accccggcac cttgaatttt ccgatggcct acgtgaagcg cccggcgccc
     4261 gccaacacgc cgaacgcgga catcgacgtg cgcgacctga tcacttcgac caccggcggg
     4321 gacctctata tccggcagca ggcgagcgtc ggttcggtcg agaccaatat caccaagtcg
     4381 atcaccatgg gcatgggtga cgtgcgcgat ctcgacgtgt cggcggacgg caagaaattg
     4441 gtgttctcgc tgcgcctgcc gctcaacccg aacaagccca acgacgacat cacgcagccg
     4501 aactggaaga tctacgagta cgacgcggtc gctcaaaccg tgacgcagct gaccaacgac
     4561 aacaccaccg ccggccatga cgtgggcgcg cactacctgc ccgacgggcg catcgtgttt
     4621 gcctcgaccc gccaggcggc gacgcaagcc attctgatcg atgaagggcg cccgcagtac
     4681 ccggcgcaga ccgatgaccg caagcaaccg attttcctgc tgcatgtcat gaacgccgac
     4741 ggctccaaca ttcatcagat cagcatgaac accaaccacg atttcgcgcc ttcggtgctg
     4801 gcgaacggtc aaatcgtgtt ttcgcgctac gagtccatca acggcgatca gatcagcctg
     4861 taccgcagca atccggacgg aaccggcctc gagctctact atggcgagaa cagccatgcc
     4921 acgggcgcca acatcgccgg caccaacaac aacgtgattc aattcttgaa tgcccggcag
     4981 cgcgccgacg gccagctgat tgcgatcgtc cggccgtttc tcggcaccca gcagggcggc
     5041 gatatcgtgc agatcaacgc cgagaatttc gtggaaatcc accagccggc caccccgggc
     5101 ggcgccgccg ggactgcgca gtcgagcgcc accacgctcg gcgtcaccac cgacgcgaac
     5161 atgccctcgc tcggcggccg tttcgcttcc gcgtatccgc tctacgacgg gaccaacaga
     5221 atgctggtga gttgggcgcc ttgcttgatc atcgatacca ccgtgacgcc gaacgccacc
     5281 aaggtttgca cggcagccaa caccagcggc gccaacgtgc agctggcgcc gccgcagtac
     5341 accgtgtgga tctacgacgt ttcggccggg accttgagtc cgattctggg cgcggagatc
     5401 aataccgtga tcgtcgagcc ggtcatcatg caggcccgca ccccggtacc cgcattcgtt
     5461 ccggacgtgg tgccgagcgg cgccgctgcc aatctcgcca acaataccaa tggcggcttg
     5521 ggaatcctcg aaatccgcag cgtctatgac ttcgacggcg tcgacgaagt atcagctgaa
     5581 acggcgggtt ccataccgaa tat
//
LOCUS       DASWZT010000397         6906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_36997, whole genome shotgun sequence.
ACCESSION   DASWZT010000397 DASWZT010000000
VERSION     DASWZT010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6906
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36997"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VGE92_11150"
     CDS             complement(<1..478)
                     /locus_tag="VGE92_11150"
                     /inference="COORDINATES: protein motif:HMM:NF015935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HEY1314428.1"
                     /translation="MTHRSLSKKLAVRSDRGYVVAPPETFSTRFFEQTETGMVPVADS
                     AVIEASLAILAKRVARGSVLSSPSAVKNFLVTRLADLEHEIFGVLLLTTRHHLIDFVE
                     LFRGTLDGANVAPREVVKLVLARNAGAVILVHQHPSAVKDASQADELITATLKEALA"
     gene            complement(766..1272)
                     /locus_tag="VGE92_11155"
     CDS             complement(766..1272)
                     /locus_tag="VGE92_11155"
                     /inference="COORDINATES: protein motif:HMM:NF042609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0758 domain-containing protein"
                     /protein_id="HEY1314429.1"
                     /translation="MLGRMAIRDWPIDERPREKLLDKGAAALSDAELLAILLRTGTRG
                     FSALDVAREVLKNFSSLRKLIAADRQRFCAERGLGLARFAELQAAVEIARRQLSETLR
                     AAPSLASPRATRDFLSAKLRDLEHEVFCCLYLDKRHRLIQFEELFRGTIDGALYLASR
                     LSIAFKCL"
     gene            complement(1330..1785)
                     /gene="dut"
                     /locus_tag="VGE92_11160"
     CDS             complement(1330..1785)
                     /gene="dut"
                     /locus_tag="VGE92_11160"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEY1314430.1"
                     /translation="MRRIKLRILDPRVGREFPLPQHATAGSAGMDLRACIDAPLTLGP
                     GAAELIPTGLSIYIADPDLAAVLLPRSGLGHKSGIVLGNLVGLIDSDYQGPLMVSLWN
                     RGGASFVINPGDRIAQMVFVPVARVEFEVVEDFAATARGAGGFGSSGHS"
     gene            complement(1788..3068)
                     /gene="coaBC"
                     /locus_tag="VGE92_11165"
     CDS             complement(1788..3068)
                     /gene="coaBC"
                     /locus_tag="VGE92_11165"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005101801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEY1314431.1"
                     /translation="MTPKTRILLGVTGGIAAYKSPDLVRRLIERGADVQVVMTGAAQR
                     FVSPMSFQAVSGRPARSDLWDDAAEAAMGHIELARWAQVVLIAPASADFIARLANGRA
                     DDLLATLCLATEAPIVLAPAMNRVMWANKATQANIETLVTRGIRILGPASGNQACGEI
                     GEGRMWEPLKLAESVLERPANEGLLSGLNILITAGPTRERLDPVRYLTNRSSGKMGFA
                     VAAAAREAGAHVTIVTGPVHLPTPAGITRINVESARDMYAAVHRQVADADVFIAAAAV
                     ADFQPVTVAKQKIKKQGTGVKLDLEPAPDIIKSVADMAKRPFVVGFAAETNDVEDNAR
                     GKLKRKKLDMIAANQVGEGIAFDCDDNALTVIWPGGLAEIARGPKIEVARQLIALIAS
                     RLPPPDGSPRRGPRKRRTAAALPRDRVRARRARR"
     gene            complement(3065..3688)
                     /locus_tag="VGE92_11170"
     CDS             complement(3065..3688)
                     /locus_tag="VGE92_11170"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEY1314432.1"
                     /translation="MRKAAGILCALTLLAAPGLAPWAGNGRTLYKWVDEQGVTHYGDR
                     IPPEYAAQEQHVINSQGIEINKLEAQKTPEQLAAEDQKRLDAEQSKNRDRNLLSTYAS
                     VQEIERLRDQRVALISDQIKVTSQFLEVLNGKMRQLRLNGMRFKPYNNDPKAPAMPDQ
                     VADDLVRVGNDIRTQEENLREKRSEEVTMTKEFESDITRFKELKGIH"
     gene            complement(3758..4408)
                     /gene="pyrE"
                     /locus_tag="VGE92_11175"
     CDS             complement(3758..4408)
                     /gene="pyrE"
                     /locus_tag="VGE92_11175"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEY1314433.1"
                     /translation="MTLAYQSEFIDLCARLGVLKFGSFKLKSGRESPYFFNAGLFSSG
                     AAIAAVGRAYAAALTASNLGFDMLFGPAYKGIPLATITAAALAERAGRDLPFAYNRKE
                     TKDHGEGGNIVGSPLRGAVLIVDDVITAGTAIRESVDIILSAGARPAGVLLALDRQEC
                     APDSRLSAVQEVRKQYGIPVIAVVSLADLMEHASLQGRDDDLKRMQIYRERYGLAD"
     gene            4463..5725
                     /locus_tag="VGE92_11180"
     CDS             4463..5725
                     /locus_tag="VGE92_11180"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1314434.1"
                     /translation="MTEAVKPTLRQALGDKRMTAVLLMSFASGLPFNLTGFTIQAWLA
                     SAGLSIKTIGIFSLVALPLNFKFFWAPLLDRYMPPFLGRRRGWIVIFQASLTICIAVM
                     GFCSPTRELYVLGAITLFLAFLSASQDIVIDAYRVDIIPPSERALAAAASAFGYRTAA
                     MLAGAALVYIAASVGWQLAFLAVACLMAATMVATIRSPEPLEPGHAPRTLAAAVWHPL
                     RALLQQKGMWGFILLVLLYKVGDALALSLYSTFMIKGVGFSLHELSIAGKLNMTISTM
                     IGVAFGGWVYIRWGTFRSLLVFGIGQALTNLLYMWLALAGKKVWLLVLATALDTMIGG
                     MGQAAFVAFLMSLCSADFSATQYALLSALATLPRTLTGAVAGELVPVIGWPRFFVVTC
                     LVAVPGLILLVALRRPLNELGAREAAKA"
     gene            complement(5734..6036)
                     /locus_tag="VGE92_11185"
     CDS             complement(5734..6036)
                     /locus_tag="VGE92_11185"
                     /inference="COORDINATES: protein motif:HMM:NF042799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6763 family protein"
                     /protein_id="HEY1314435.1"
                     /translation="MNAVKPVVGQWYRGGTNELFEVVAIDDQDQTIEIQYFDGTVAEM
                     EFDSWNEQLLDEQIDAADAPEDWSGAVDVEAEDLDRQFEDNARGAWSIPHDRSMRR"
     gene            6168..6746
                     /locus_tag="VGE92_11190"
     CDS             6168..6746
                     /locus_tag="VGE92_11190"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEY1314436.1"
                     /translation="MVDPISLIPLFAGLTQGASNRYKKKMAGKAAAIALVICVLFALV
                     GAKFLDIMGISLNAFRIAGGTLLFLIALDMVFARPSGTRSTSSEREEAKNREDISVFP
                     LAFPFIAGPGALATILLTAGEVASKPLLFAGFLGVVALVVIVCWVLMLATPRLMKVLG
                     VTGANVTSRLSGVILAALAVQFIIDGIHGSFL"
     gene            complement(6741..6906)
                     /locus_tag="VGE92_11195"
                     /pseudo
     CDS             complement(6741..6906)
                     /locus_tag="VGE92_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606533.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
BASE COUNT         1213 a         2154 c         2103 g         1436 t
ORIGIN      
        1 gcgcgagcgc ctctttgagc gttgccgtga tgagttcgtc ggcttgtgat gcgtctttta
       61 cagcactcgg atgctgatgg acgaggatga cagcgcctgc gttccgagca agaactagct
      121 tgacgacctc acgaggtgcc acgttcgcgc catccaaagt accgcggaaa agctcgacga
      181 agtcgatgag atgatggcgc gtcgtgagca gcagcacgcc gaagatctca tgctccaggt
      241 ccgcaagccg cgttaccagg aagtttttga cggcgctcgg agagctgagc accgaaccgc
      301 gtgccacgcg cttcgcgagt atggcgagag acgcttcgat gaccgcagag tcggcaactg
      361 gcaccatgcc ggtctcggtc tgctcaaaga atcgggtgct gaaagtctcc ggcggagcga
      421 caacgtagcc gcggtctgag cgaacggcaa gctttttaga gagtgagcga tgagtcatgt
      481 gagtctcctt gtagccggcg aaatggccgg cggggagaac ccgcacgtcg cagcggagcg
      541 ctcaattgca ccctggaata aaatggaggg gacccgcccc ctgcggggag caggccgttt
      601 tatgccggga aaggcgattg accgcgacga ccaacgtgac acagtcaaac ccccgacaat
      661 cgagccaatc cacgctgcca aaacgtaaat aagtacgctc tcagagcgtt cctatttaca
      721 aatccccaaa tcgcgctacc atcgagcaaa acaaaaacac tgcatttata ggcatttgaa
      781 cgctattgac aagcgacttg ccagatataa cgcaccgtcg atcgtgccgc gaaacaactc
      841 ctcgaactga atcagccgat ggcgcttgtc caggtacagg cagcagaaca cttcgtgttc
      901 caaatcgcgc aatttcgcgg agagaaagtc tcgcgttgcc cgtgggctgg cgagcgaagg
      961 agcggcgcgc agggtctccg acagttgcct gcgggcaatc tcgaccgcgg cctgcaattc
     1021 cgcgaagcgt gccaatccaa gcccgcgctc ggcgcagaat ctttgccggt cggcggcgat
     1081 caacttgcgc agcgagctaa aattcttgag cacctcgcgg gccacatcga gagcagaaaa
     1141 accgcgggta cccgtgcgca atagaatcgc cagcaattct gcgtccgaca gagccgcggc
     1201 ccccttgtcg agcagctttt cgcgcggccg ctcatcgatc ggccaatctc gaattgccat
     1261 gcggccgagc atgagcggcg cgcccgcaac tctccaggcg catttcgccg gcgtacgcgc
     1321 agattggcgt cagctgtgtc ccgaactgcc gaagccgccc gcgccgcgtg ccgtcgccgc
     1381 aaagtcctcg accacttcga attcgacccg agccacgggc acgaacacca tttgggcgat
     1441 gcggtcgccg ggattgatga cgaaggaagc gccgccccga ttccacagcg acaccatcaa
     1501 cggcccttga taatcggagt cgatcagccc gaccagattg ccgagaacga tgccgctctt
     1561 gtgtccgagt cctgaacgcg gcagcaacac ggccgcaaga tccgggtcgg cgatgtagat
     1621 cgacaggccg gtcggaatga gctcggccgc gccgggtccg agggtgagcg gtgcgtcgat
     1681 gcaggcgcgc aagtccatgc cggcggagcc cgccgttgcg tgttgcggca gcgggaattc
     1741 tcgtccgacg cgcggatcca agatccgcag cttgatccgt cgcacgctca acgccgcgcg
     1801 cgccgcgcgc ggacgcggtc gcgcggtagg gccgcggcgg tgcggcgctt gcgcggcccg
     1861 cggcgcgggc tgccgtcggg cggcggcagg cggctcgcaa tgagggcgat gagctggcgg
     1921 gctacttcga tcttcggtcc gcgtgcgatc tcggccaggc ctccgggcca gatcaccgtg
     1981 agcgcgttat cgtcgcagtc gaatgcaatg ccttcgccca cttgattggc ggcaatcatg
     2041 tcgagcttct tgcgcttcaa tttgccgcgc gcgttgtcct ccacatcatt ggtctcggcc
     2101 gcaaatccga ccacgaaggg tcgcttggcc atgtccgcaa ccgacttgat gatgtcgggc
     2161 gccggctcga gatccagttt gacgccggtt ccctgcttct tgatcttttg tttggcgacc
     2221 gtcaccggtt gaaaatccgc caccgccgcg gctgcaatga agacgtcggc gtccgccact
     2281 tgccgatgaa ccgcggcata catgtcgcgc gcactttcca cgttgattcg cgtgatacct
     2341 gccggcgttg gcaagtgcac cggcccggtg acgatggtga cgtgagcgcc cgcttcgcgc
     2401 gcggcggccg cgaccgcaaa tcccattttt ccggagctgc ggttggtgag atagcggacc
     2461 ggatcgaggc gttcccgggt gggacccgcg gtgatcaata tattgagtcc cgacagcaag
     2521 ccctcgttgg ccggccgctc gagcacactc tcggccagct tcagcggttc ccacatgcgg
     2581 ccctcaccga tctcgccgca cgcctgattg ccggacgcgg gtcccaagat gcgtatgccg
     2641 cgcgtaacca gggtttcgat attcgcctgc gtggccttgt ttgcccacat tacccgattc
     2701 atggccggcg cgagaacgat cggcgcttcg gtggcgaggc acaaggtggc cagcagatca
     2761 tcagcccgcc cgttggcgag ccgcgcgatg aagtcggcgc tcgccggcgc gatgagcacc
     2821 acttgagccc agcgcgccaa ttcgatgtgg cccatggccg cttctgctgc gtcgtcccac
     2881 agatcgctgc gggcgggccg accggagacc gcttgaaagc tcatcggcga gacgaagcgt
     2941 tgcgccgcgc ctgtcatcac cacctgaacg tccgcgccac gctcgatcag tcgacgcacc
     3001 aagtccggac ttttgtacgc ggcgatgccg cccgtgacgc ccagtaagat tcgagtcttc
     3061 ggcgtcaatg gatacccttg agctctttga agcgagtaat gtcgctttcg aactccttgg
     3121 tcatcgtcac ctcttcgctg cgcttttcgc gcagattctc ctcctgcgtg cggatatcgt
     3181 tgccgacgcg gaccaagtcg tcggccactt ggtccggcat ggccggagcc ttcggatcat
     3241 tgttgtaggg tttgaaacgc atgccgttca aacgcaattg ccgcatcttg ccgttcaaga
     3301 cttcgagaaa ttgcgaggtc accttgatct gatcggagat caacgccacc cgctgatcgc
     3361 gcaggcgttc gatttcctgt acggatgcat acgtactcag caggttacgg tcgcggttct
     3421 tgctctgctc ggcgtcgagc cttttctggt cttccgcggc cagttgctcg ggagtttttt
     3481 gtgcctcgag cttgttgatt tcgatgcctt gagaattgat gacatgctgt tcttgcgcag
     3541 cgtattccgg aggtatgcga tcgccgtagt gcgtaacccc ttgttcgtcc acccatttat
     3601 agagggttcg gccattgccc gcccatggcg cgagccccgg cgcggcgagt agcgtcaggg
     3661 cgcagaggat gccagcagct ttgcgcaagt gcatatccgt tatgacaaac gaccgccatt
     3721 gtgagcggtg ggcagcgcct tttctagggc ctgtgcctca atctgcgagg ccatagcgct
     3781 cccggtagat ttgcatgcgc ttgaggtcat cgtcgcggcc ctgcagcgaa gcgtgctcca
     3841 tgaggtcagc caaactcacg accgcgatca ccggaatccc atactgcttg cgcacttctt
     3901 gcaccgccga taaacggctg tccggggcgc attcctggcg gtcgagcgcc aacagtaccc
     3961 cggcaggccg tgcccccgcg ctcaggatga tgtcgacgga ttcgcggatc gccgtgcccg
     4021 ccgtgatcac gtcatcgacg atcagcacgg ccccgcgcaa cggactgccg acgatgttgc
     4081 cgccttcacc atggtccttg gtttccttgc ggttgtaggc aaacggcagg tcgcgaccgg
     4141 cacgttcggc aagagccgcc gccgtgatgg tggccagcgg aatccccttg taagcggggc
     4201 cgaacagcat gtcgaagccg agattggacg ccgtgagcgc cgccgcatag gcgcgaccca
     4261 ccgcggcgat ggcggcgccg ctgctgaaca gtccggcatt gaaaaagtac gggctttcgc
     4321 gtcccgactt caatttgaaa ctgccgaact tgagcacgcc gagccgcgcg cacagatcga
     4381 tgaactcact ttgatatgcc aaggtcatgg gggctgatat tatcgcgcgg gatgggcacc
     4441 gtcatgtttc gagctgtcgc gtttgactga agcggtgaag cccactctgc ggcaagcgct
     4501 tggcgacaag cgcatgacgg ccgtgctctt gatgagtttc gcgtccggtt taccgttcaa
     4561 cctgaccggc tttaccattc aggcgtggct ggcgtccgcc ggactcagca tcaaaaccat
     4621 cggtattttt tcgttggtgg cgctgccgct caacttcaag ttcttttggg cgccgctgct
     4681 ggatcgctac atgccgccgt tcctgggacg ccggcgcggc tggatcgtca tcttccaggc
     4741 gtctctgacc atctgcatcg cggtcatggg tttctgctcc ccgacccggg agctgtacgt
     4801 attgggcgcc atcaccttgt tcttggcgtt cttgtcggcg tcgcaagaca tcgtgatcga
     4861 cgcctatcgc gtcgacatca ttccgcccag cgagcgggct ctcgctgccg cagccagcgc
     4921 attcggctat cgaacggcgg ccatgctcgc gggcgcggcg ctcgtctata tcgccgccag
     4981 cgtcggctgg cagctcgcct tcttggcggt ggcatgcctc atggcggcga ccatggtcgc
     5041 aacgatccgc tcaccggaac ccctcgagcc gggacatgcg ccgcgcaccc tggccgctgc
     5101 cgtctggcat cctttgcgcg ccctgctgca gcaaaagggc atgtggggtt tcatcttgct
     5161 ggtgcttctc tacaaggtgg gcgacgcgtt ggcgttgagc ctgtacagca cgttcatgat
     5221 caagggggtc ggattttcgc tgcatgaatt gagcatcgcc ggcaagttga acatgaccat
     5281 ctcgaccatg atcggtgtcg ccttcggcgg ttgggtctat atccgctggg gcacgtttcg
     5341 ctcgctgctg gttttcggca tcggtcaggc actgaccaat ctgctctaca tgtggctcgc
     5401 gctcgccggg aaaaaagtct ggctgttggt gctcgcgaca gcgctcgata ccatgatcgg
     5461 cggcatgggc caggcggcgt tcgtcgcgtt cttgatgtcg ctttgttcgg cggacttcag
     5521 cgccacgcaa tacgcattgt tgtcggcgct cgcgaccctg ccgcggacgc tgaccggcgc
     5581 agtcgccggc gaactcgttc ccgtgatcgg ttggccgaga tttttcgtcg tcacctgctt
     5641 ggtcgcggtg cctggattga tcttgctggt ggcattgcgc aggcccctga acgaactcgg
     5701 ggcacgcgag gccgcaaagg cttgaggttt gcgtcagcgg cgcatcgagc gatcgtgcgg
     5761 aatggaccac gcgccgcggg cattatcctc gaactgccga tccaagtcct cggcttccac
     5821 gtcgacggcg cccgaccaat cctccggcgc atccgccgcg tcgatttgct catcgagcag
     5881 ctgctcgttc caggagtcga actccatctc ggcgacggtg ccgtcgaaat attggatttc
     5941 aattgtttga tcttggtcgt cgattgcgac aacttcaaag agctcgttgg taccgcctcg
     6001 ataccattgc ccaacgaccg gcttgactgc gttcatcacc gcctccgtct gctatgctca
     6061 acaccattca actcccaatg agtcattcgc gcaatatgta atttttgcgc gcagcaacat
     6121 cgccagtgat cgaacagctc gttaagttct ttctcgtgtt tttcgtcgtg gtcgatccca
     6181 tctctctcat cccgctattc gcaggcctta cgcagggcgc gagcaatcgt tacaagaaaa
     6241 aaatggccgg caaagcggcc gcgatcgcgc tggtaatttg cgtgttgttt gcgttggtcg
     6301 gcgcgaaatt tttggacatc atggggattt cccttaacgc gtttcgcatc gcgggcggga
     6361 cgctgttgtt tctgatcgct ctcgacatgg tgttcgcgcg tccttcaggc acgcgctcga
     6421 ccagttcgga acgcgaggag gccaagaacc gcgaggacat ttcggtattt ccgctcgcat
     6481 ttccgttcat tgcgggcccg ggggcgctgg cgacaatttt gctgaccgcc ggcgaagtcg
     6541 ccagcaagcc gctgctgttc gccggtttct tgggcgtcgt cgcgctggta gtcatcgtct
     6601 gctgggtgtt gatgctcgcc acgccgcgct tgatgaaggt gctgggcgtc accggtgcca
     6661 acgtcacgag ccgcctatcc ggcgtgattc tcgcggccct cgcggtgcaa ttcatcatcg
     6721 acgggattca cggcagcttt ctttgagtag gtcaaatccc acacgccgtg cccaaggcgt
     6781 gcgccgcgcc gttcgaagcg cgtcagcggc ctgaattccg gccgcgcgac gtaggttcga
     6841 tcggcgctct gcgaagcgag atcggcgctg gcgttgcata ccgccaacat ctgctgcgca
     6901 tagtcc
//
LOCUS       DASWZT010000398        11894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_7907, whole genome shotgun sequence.
ACCESSION   DASWZT010000398 DASWZT010000000
VERSION     DASWZT010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11894
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7907"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1592)
                     /locus_tag="VGE92_11200"
     CDS             complement(<1..1592)
                     /locus_tag="VGE92_11200"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY1314437.1"
                     /translation="MTATGAAPLSYQWQKNASPIAGATQSSYTTPVTSPADNGTSFAV
                     AVGNSSGSTLSRSALLSVTNGAAPAGTDVLTYKNDLSRTGLNPTESLLTPANVSSATF
                     GLLRNLAVDGKVDAQPLYVSQLSISGSNHNVVFAATEHDSVYAFDADTGSLLWRVTLL
                     ASGETLSDTHGCSQVTPELGITATPVIDRAAGAHGTLYVVAMSKDAASNYHQRLHALD
                     LASGAELLNGPVEISATYPTSGGGMSAFLPGQYEERAALLLLNGTLYTSWTSHCDISP
                     YFGWVIAYSQSSLARTAVLNVAPNSGGAGPAIWMSGGGPAADSAGNIYLLTANGVFET
                     SLDANGFPNLQDYGNSFLKLSTAGGSLSVADYFTMFNEVAESGADQDLGSGGELLLPD
                     LTDSTKTVRHLAIGAGKDGNIYLVNRDSMGKFNSANNSQVWQQLSGALPGGIWSTPAY
                     FNGTVYFGEVGDTLKAFAISNAQLAAAPQSQSATQFAYPGTAPCVSANGTANAIVWAH
                     ENSSPAVLHAYDAANLGRELYN"
     gene            1905..2612
                     /locus_tag="VGE92_11205"
     CDS             1905..2612
                     /locus_tag="VGE92_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314438.1"
                     /translation="MSKDDELGLTVFEDVIPQPLLDRMNAVIDATRDRWSEADGGSET
                     RLAGSFADMLRCNNRFRLIEQGAFAAVTTYPAAPIPTADSKHVLRCVNRDMAWQSHLR
                     HHDSHLLTLLIPLQLAGGTQQNGDLLLYRKRRQSVSLVSNLIYKTWLVIQQNRRFATR
                     KRQTYRDIERQHCRRIACKPGNVYVFNGFLSLHANLEIESGERRSLIIHHFDPRLTAG
                     ITNVTRTLRSVRERLTP"
     gene            complement(2613..3458)
                     /locus_tag="VGE92_11210"
     CDS             complement(2613..3458)
                     /locus_tag="VGE92_11210"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEY1314439.1"
                     /translation="MRQFPADERPIAIQPELRRAQRACLAATPSPIVRLTRVYVGAAL
                     MPGSVVDLPRDTASHLAKVLRARDGDELILFNGDGGEFLGTIEAVRGSRVSASVGESR
                     AVDRESPLRITLLQCVPRGDRMDLIVQKATELGVARIVPALSQRSVVRLDRAQAESKL
                     VHWRAVAVSACEQCGRNRLPIIEAAQPLLHYLGEPATATALRLVFEPESAPQAFGSAL
                     ESGGAALGAVEIAIGPEGGFASDELEAFRVAGFSQVGLGPRILRTETAAIAALVWLQT
                     RFGDL"
     gene            3490..4071
                     /gene="ampD"
                     /locus_tag="VGE92_11215"
     CDS             3490..4071
                     /gene="ampD"
                     /locus_tag="VGE92_11215"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005108195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEY1314440.1"
                     /translation="MDLQVDLESGLLRGARQIASPNFDSRPAGVETDLIVVHGISLPP
                     GEFGGPWIDRLFTNTLPPQQHPYFAEVAPLRVSSHLVLDRDGKVTQYVRFTDRAWHAG
                     KSVYKQREACNDYSVGVELEGTDTLPYAAAQYGALAEVVAALCAAYPRLSARRLVGHS
                     DIAPGRKTDPGPAFDWPRARRLIASACRRTRRR"
     gene            4186..7005
                     /locus_tag="VGE92_11220"
     CDS             4186..7005
                     /locus_tag="VGE92_11220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1314441.1"
                     /translation="MIRWFSDLPIERKLRVVIMVPASAVFAVAMAAHIAMNLLHLRDD
                     LQWSAARVARTTGVNTLEALRRGDDKVALRAMGSLRDEWMVSDAEVYGSDGRRLALYR
                     RGHNDAQLESAPKPDLAAVPRPAPLSDPQHPRLFLRGSQFHITAAVMRNDEVLGFVHI
                     LVPLEVLYPDWQSYLLITLAAIVAAVLSAYWLAARLQQQISGPIVNLARTMQRVSTEQ
                     DYSLRVERSSQDEVGSLIDGFNQMLGQIRHRDSRLEKYRHYLEQQVEERTLNLANANL
                     GLKSAIGEANRAKEAAERASRAKSEFLARMSHEIRTPMNGVMGMSELLQATDLTPRQR
                     HLSETISHSAGALLQIINDILDFSKVEAGKLKLEFVDFGLRDAVEGTLEIFAGRAHAK
                     HVELVCALELDLPGTVCGDPMRLRQILINFVGNAIKFTDAGEVIVRVRALDPRGLLRF
                     EVTDTGIGISAEAQDAIFSPFNQADSFTTRKYGGTGLGLAICRQLVALMGGKIGVHSE
                     VNRGSTFWFEVRLPPTANGSQTLTRLPRFNLVGLRALVVDDNASNRHILQQHLESWGA
                     EAAGAASGAAALALLNAGAQTPFDLVLLDDQMPGMDGMQLARLIVADPRRASLRLILL
                     STREDADGESSGSALFAAILAKPLRRAQLFACVSRAMTMQPESAAAAASPGSTHNNGP
                     PAVVAPKVLLVEDNAVNREVAVGMLESLGCTTDTAANGRLAIEAMSCSTYDVVLMDCQ
                     MPVMDGLTATEEIRRREQSSGAPHIPIVALTANAMDGDRERCLARGMDDFLSKPFTRQ
                     QLATLLRRWLALRTLPETERHDLSQVPLIDAGVLRDIAALGKPALLDSMIEFYLQESP
                     RLLATIDSAAAAMRFDALLQAVHSLKSSTANLGGSRLALVAKECESLVREGAIAQVAS
                     LVSRIHSEYRDFCAALLQEQSRSAA"
     gene            7030..8844
                     /locus_tag="VGE92_11225"
     CDS             7030..8844
                     /locus_tag="VGE92_11225"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY1314442.1"
                     /translation="MNTTDYKGIVPATVLVVDDDPGARLLLGTALEIAGFDVITAEDG
                     LAALHRFRERPADCVVLDVVMPGMNGFDVCSALRRLPTCGHVPILMQTSLDDMQSIGR
                     AYDAGATDFSSKGINPMLLAQRVKFLVRAKQTQDQLRESEARVRYLAYYDPLTGLPNR
                     QRLLQILQQQVEWATPRRRGIAVLMLDVDNFSRINDTQGQAAGDALLKEVAHRLQYCL
                     RDAQRMLESAADSLDTKDINDWVARTGGDEFALALPGAATAEAAQVVARRVQAALARP
                     FISALQEFPLSASMGISLFPGDATTAESLVKHADAAMHHGKKSGRGGVEFFNKSISTQ
                     AARHLSMEADLRKALERGEFTLHYQPRLALKDLRVEAVEALLRWKHPQRGSVAPDEFI
                     PLAEQSGLITEIGDWVLREACAQVRRWRDAGAPDWQVAVNVSGVQFRDGSLVTRVSRA
                     IDAAAIDSRMLELEFTEGALIEYSAAVSKAVKSLKELGVATALDDFGTGYSSLSYLRH
                     FPIDTLKIDRSFVRDIASKTGGNAPLVDAIIAMAKSLGLATVAEGVESEAQWRYLKTR
                     EANQVQGFLFARPMAIAELQRWHAEWLDTKQLAALSAA"
     gene            complement(8841..9398)
                     /locus_tag="VGE92_11230"
     CDS             complement(8841..9398)
                     /locus_tag="VGE92_11230"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004647509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY1314443.1"
                     /translation="MGNRLSKIYTRTGDDGSTGLGDGTRVPKEDLRVEAYGTVDEANS
                     AIGVVLGVGALPEPVAHCLIEVQHDLFDLGGELCIPGHRMIGAAHVDRLENQLDGFND
                     GLPPLKEFILPGGGPAAAACHVARAVCRRAERRCWSLARTATVSPEALKYLNRLSDLL
                     FVVARVLARHENGSEVVWRRTNNKP"
     gene            9458..10222
                     /locus_tag="VGE92_11235"
     CDS             9458..10222
                     /locus_tag="VGE92_11235"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY1314444.1"
                     /translation="MKHSAELQAALDAAEQAAAIARAMFEHNVEVRIKADKSPVTEAD
                     VRCEIAIREILEQRFPTYGFFGEETGSRDTQAESLWLVDPIDGTKAFVREYPMFSTQI
                     ALMRRGEFVLGVSSAPVYGELCYAERGRGTFLKGKPAHVSHVAMLESAALSAGNVRSL
                     AMGSGWARYGALVAQVDRIRGYGDFLHYHLLASGKIDAVIESDVNILDIAACAAIVTE
                     AGGRFTDLKGAAISLESTSVLATNGRLHDAVLAALG"
     gene            complement(10231..11079)
                     /locus_tag="VGE92_11240"
     CDS             complement(10231..11079)
                     /locus_tag="VGE92_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314445.1"
                     /translation="MSRSRGTPPKRGTASSGGAAPSRNGWKTLRVAILLAVLVVVALT
                     TWQDRYLSTRWHNPLYVAIYPIAADDSPVTRAYVTALDAERFKPIDRFFAREAARYHL
                     DTSEPIKTRLRAELQDRPPQREPNAGLLATAFWSLRLRYWAWRASGHVHEPEDIRIFV
                     LYHDPALTPTVPHSLGLTKGLIGVVYAFAAPEMNGENDVVIAHELLHTVGATDKYNLA
                     DDAPRFPDGYGDPAQVPRYPQLTAELMAGRRVLSPDKWRQAVSLDEVVIGNATALEIR
                     WPQQPR"
     gene            complement(11076..11264)
                     /locus_tag="VGE92_11245"
     CDS             complement(11076..11264)
                     /locus_tag="VGE92_11245"
                     /inference="COORDINATES: protein motif:HMM:NF025614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HEY1314446.1"
                     /translation="MNLLQDQDAACPHCWETINLTLDLSVPEQSYIEDCPVCCKPMSI
                     SYRSVDGELAEFSIESGE"
     gene            11378..>11894
                     /locus_tag="VGE92_11250"
     CDS             11378..>11894
                     /locus_tag="VGE92_11250"
                     /inference="COORDINATES: protein motif:HMM:NF026042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEY1314447.1"
                     /translation="MEPTDTSKSDYFHRVVDCQWACPAHTDVPEYIRLIAQGEFTEAY
                     MVNRESNVFPGILGRVCDRPCEPACRRGRIEAKPVAICRLKRVAADHRDDISALLPKA
                     PREKNGKRVACIGAGPASLTVANDLLPMGYEVTIFEQYAVPGGLMRTNIPAFRLPAAV
                     LDEEIGYVVDMG"
BASE COUNT         2198 a         3666 c         3852 g         2178 t
ORIGIN      
        1 ctgttgtaga gctcgcggcc gagattggcg gcgtcatacg catgtagaac tgcggggctg
       61 gaattctcgt gcgcccagac gatggcgttg gcggtgccgt tggcggatac gcagggcgcc
      121 gtacccggat aggcgaactg cgtggcggat tgcgactgcg gcgcggcggc cagttgcgca
      181 ttcgaaatcg cgaaggcctt gagcgtatcg ccgacttcgc cgaaataaac agtgccgttg
      241 aaatacgccg gtgtggacca aatgccgccc ggtaaggcgc cgctcaactg ttgccagact
      301 tgcgaattgt tcgccgagtt gaatttgccc atcgagtcgc gattcaccag gtagatgttg
      361 ccgtccttgc cggcgccgat tgccaggtgt ctgacggtct tggtcgaatc ggtcaagtca
      421 ggcagcaaca gttcgccgcc tgaacccaga tcctggtccg cgcccgactc cgcgacttcg
      481 ttgaacatcg tgaagtaatc ggcgacgctc aacgagccgc cggcggtcga tagtttgaga
      541 aaggaattgc cgtagtcctg cagattggga aatccgttcg cgtcgagcga ggtttcaaag
      601 acgccgttcg ccgtcaataa atagatgttg ccggccgaat cggcggcggg accgccgccg
      661 ctcatccaga tcgcagggcc ggcgccgccg ctgttgggcg cgacgttgag caccgcggtg
      721 cgtgccagag agctttgcga ataggcgatg acccagccaa aataagggga gatgtcgcaa
      781 tgcgacgtcc agctggtgta gagggtccca ttcagcagca gcagcgctgc ccgttcctcg
      841 tactgacccg gcaagaacgc gctcatgccg ccgccggatg tggggtaggt cgcggagatc
      901 tccaccggtc cattcagcaa ttcagctccc gatgcgagat cgagcgcgtg cagccgctgg
      961 tgataattcg aggcggcgtc tttcgacatg gcgaccacgt acaacgtgcc gtgcgcgccc
     1021 gcggcgcggt cgatcacggg tgtcgcggtg atgccgagct ccggagtgac ctgactgcag
     1081 ccgtgtgtat cgctcagggt ttcgccgctc gcgagcagcg tcacgcgcca tagaagcgag
     1141 cccgtatcag cgtcgaaggc gtagacggaa tcgtgctcgg tggctgcgaa caccacgttg
     1201 tggttcgatc ccgagatgct cagttgcgaa acatagagcg gctgtgcgtc caccttgcca
     1261 tcgaccgcca agttgcgcag caatccgaag gtcgcagaac tcacattggc aggggtcagg
     1321 agcgactcgg tgggattgag tcccgtgcgg ctcaggtcat tcttgtacgt gagcacatcc
     1381 gtacccgcag gcgcggctcc attcgtcacg ctgagcagcg cactgcggct taacgtactg
     1441 ccgctggaat tgccgacggc gaccgcaaag ctcgtgccgt tatcggcagg cgaggtcacg
     1501 ggagtcgtgt agctcgactg ggtggcgccg gcgatgggcg aggcattctt ctgccactga
     1561 tagctcaagg gagccgcgcc cgtggctgtc accgtgaaag tcgcgctttg tccggccggc
     1621 acgctctggt cggcgggctg aatggtaatg gcgggggtgg aagacgcggg ggttggcgga
     1681 gtggtagccg ctgttcgatt gcccccgccg ccacatgccg cgagcagaag cgaagcggcc
     1741 agcgcagaca aaagtccagt ctttgacata acttgccgta tgtggctcat gtagtccatc
     1801 atgcccctcc cgggagaatg cgagaaaggg aattcgtggg atttgtatcg gacgaaattg
     1861 accaagccac cgttgttgcg ggccctgccg gggtcgccgg ccgtgtgagc aaggacgatg
     1921 agctgggttt gactgtcttc gaggacgtca ttccgcagcc gctgctcgat cggatgaacg
     1981 ctgtgatcga tgcgacgcgc gaccgctggt ccgaagccga cggcggctcc gagacccggt
     2041 tggcgggatc ctttgcggat atgctgcggt gcaacaaccg cttccgcttg atcgaacagg
     2101 gtgccttcgc ggccgtcacg acgtatccgg cggcgccaat tccaaccgcg gactcgaagc
     2161 acgtgctgcg ctgtgtcaat cgcgacatgg cgtggcagag tcacttgcgg catcacgatt
     2221 cccatttgtt gacgctgctg atccccctgc agctggcggg cggcacgcag caaaatggcg
     2281 acctgctgct ctacaggaag cggcgtcagt cggtctcgct ggtttcgaac ctgatctaca
     2341 agacgtggct ggtcattcag cagaatcgcc gtttcgcgac gcgcaagcgg cagacctatc
     2401 gcgatatcga gcgccagcat tgccggcgga tcgcctgcaa gccgggcaat gtgtatgtct
     2461 ttaacgggtt tctatccttg cacgccaacc tcgaaatcga atccggcgag cgtcgcagcc
     2521 tcatcatcca ccacttcgat ccgcgcctta ccgcgggcat caccaacgtg acaagaacgc
     2581 tgcgcagcgt gcgcgagcgt ctgacacctt aactacaagt cgccgaatcg cgtttgcagc
     2641 cagaccagcg ctgcgatggc ggcagtctcg gtccgtaata tccggggtcc caacccgacc
     2701 tgagagaatc ccgcgacccg gaacgcctcg agttcgtcgg atgcgaagcc gccctccggt
     2761 ccaatcgcga tctcgacagc gccgagcgca gcgccgccgg actcaagcgc gctgccgaat
     2821 gcctgcggtg cggactcagg ctcaaacacc agccgcagcg ccgtcgccgt cgccggttct
     2881 cccaagtagt gcaggagcgg ctgtgcagct tcgatgatcg gcaggcgatt gcgtccacac
     2941 tgttcgcacg cactcaccgc cacggcccgc caatgaacga gtttcgattc tgcctgagcc
     3001 cggtcgagcc ggacgacgct gcgttggctc aaggccggca caattcgcgc aacgcccaac
     3061 tcggtcgctt tttgcacgat gaggtccatt cgatcgccgc gcggcacaca ttgcagcagc
     3121 gtaatacgca agggcgattc gcggtccacg gcgcggctct cgccgaccga ggcagacact
     3181 cgagagcccc gcacggcctc gatcgtcccc aagaattcgc cgccgtcgcc gttgaataga
     3241 atcaattcgt cgccatcgcg cgcgcgcagc accttggcca gatgcgacgc ggtatcgcga
     3301 ggcaggtcga ccacgcttcc gggcatcaac gccgccccga catacacccg agtcaagcgc
     3361 acgatcggag atggcgttgc cgcaaggcag gcgcgctgag cgcggcgcaa ttcaggttgt
     3421 atggcgatcg ggcgttcatc tgcggggaat tgtcgcatat gctaacggct ctatcggcaa
     3481 ccgaggggcg tggacttgca ggtcgacctt gagagcggac tcctgcgtgg cgccagacaa
     3541 atcgcgtcgc ccaacttcga ctcgcgacca gccggggttg aaaccgatct catcgtggtc
     3601 cacggtatca gccttccccc aggcgaattc ggcggccctt ggatcgaccg gctgttcacc
     3661 aataccttgc cgccgcaaca gcacccctat ttcgccgagg tggcgccgct gcgcgtgtcg
     3721 tcccacttgg tgctcgatcg cgacggcaag gtcacgcagt acgtgcgttt caccgaccgc
     3781 gcctggcatg ccggcaagtc ggtttataag caacgcgagg cctgcaacga ctactcggtc
     3841 ggtgtggaac tggaaggaac cgataccctg ccgtacgcgg cggcccagta cggcgcgctc
     3901 gcggaagtgg ttgccgcgct atgcgccgcc tatcctcgac tgtctgcacg gcggttggtg
     3961 ggtcacagcg atattgcgcc cggccgcaaa accgacccag gaccggcttt cgactggcca
     4021 cgagctcggc gcctgatcgc gagcgcgtgc cgacgaacgc ggcgacgcta gcaggaccgc
     4081 tgcaggcgag gctgccggat gtgaagtccg tcacactcta gatttccgcc gccgggccga
     4141 cacttagctg ggacctgcta gaaccgtgtc gcgcccggag gcctattgat tcgctggttt
     4201 tcggaccttc ccatcgaacg caagttgcgc gtggtcatca tggtgcccgc gagcgcggtg
     4261 tttgccgtcg ctatggcggc gcatatcgcg atgaacctgc tgcacctgcg cgacgatttg
     4321 caatggagcg cagcgcgcgt cgcccgaacc accggcgtga acaccctcga ggcgttgcgg
     4381 cgcggcgatg acaaagtcgc actgcgggcg atgggcagcc tgcgcgatga gtggatggta
     4441 agcgatgctg aggtctatgg atcggacggc cggcggctcg ccctgtaccg ccgcggccac
     4501 aacgatgcgc agctggaatc ggctcccaag ccggacttgg ccgcggtacc gcgcccggcg
     4561 ccactctccg atccgcagca tccgcggctg ttcctcaggg gtagccaatt tcatatcacg
     4621 gccgcagtga tgcgcaacga cgaggttctg ggcttcgtgc acatcctcgt gccgctcgag
     4681 gtgctgtatc cggattggca gagctacctt ctcatcacac tcgcggcgat cgtcgccgct
     4741 gtgctcagcg cctactggct cgccgcgcgc ctgcaacagc aaatttcggg gccgatcgtg
     4801 aatctggcgc gcaccatgca acgcgtgtcc acggagcagg actacagtct gcgagtcgag
     4861 cgcagttctc aagatgaggt tggatcgctg atcgacggct tcaaccagat gctcgggcag
     4921 attcgacatc gcgattcgcg tctcgagaaa taccgccatt acctcgagca gcaagtcgag
     4981 gaaagaacct tgaatctggc gaatgccaac ctcggcttga agagcgctat cggcgaagcc
     5041 aatcgcgcca aggaggctgc ggagcgggcg agcagggcca agagcgaatt cctcgcccgc
     5101 atgagccatg aaatccgtac gccgatgaac ggcgtcatgg gcatgtcgga actgctgcag
     5161 gcgaccgatc tcacgccacg gcagcgacac ctttcagaaa ccatttcgca ttccgccggg
     5221 gcgttgctgc agatcatcaa cgatatcttg gacttctcga aggtcgaggc gggaaagctg
     5281 aaactcgagt tcgtcgattt cggtctgcgc gacgccgtcg aaggaacatt ggaaatcttc
     5341 gccggccgcg cgcacgcgaa gcatgtggag ctggtttgcg cgctggaact cgatctgccg
     5401 ggcacggtgt gcggcgaccc catgcggctg cggcagatcc tgatcaactt cgtcggcaac
     5461 gccatcaaat tcaccgatgc gggcgaagtg atcgtcaggg tcagggcgct cgacccgcgc
     5521 ggcttgctgc gcttcgaagt aaccgacacc ggcatcggaa tatccgcgga agcgcaagac
     5581 gccatattca gccctttcaa tcaggccgat tcgtttacga cgcgcaagta tgggggaacc
     5641 gggctgggac ttgccatttg caggcaactg gtcgcgttga tgggcggaaa gatcggcgtg
     5701 cacagcgaag tcaatcgcgg ctcgacattt tggttcgagg tgcggctgcc gcccaccgcg
     5761 aacggcagcc agaccctgac gcgcttgcca cgattcaatc tcgtcggctt gcgcgcgctg
     5821 gtcgtcgacg acaacgccag caatcggcac atactgcaac aacacctgga atcctggggc
     5881 gcggaggcgg ccggcgccgc cagcggcgcc gcagcgctgg cgctgttgaa tgccggcgcg
     5941 caaacgccct tcgatctggt gctgctggac gatcagatgc cgggcatgga cggaatgcag
     6001 ctggcgaggc tcatcgttgc ggatccgcgc cgcgcgtcgt tgcggctgat tttgttgagt
     6061 acacgcgaag acgcggacgg ggagagcagc gggtcggcat tgttcgctgc gattcttgcc
     6121 aagccgctgc gccgcgcgca gctctttgca tgcgtgagcc gcgcgatgac gatgcagccg
     6181 gagagcgctg cggcagcggc aagtcccgga tcgacgcata acaacgggcc gccggccgtg
     6241 gttgcgccga aagttctgct cgtcgaggac aacgcggtga accgcgaagt cgcagtcggg
     6301 atgctggaga gcttgggttg caccacggat acagcggcga acggccggct tgccatcgaa
     6361 gcgatgagtt gctcgaccta cgacgtggta ctgatggact gtcagatgcc ggtgatggac
     6421 ggattgacgg cgaccgagga aatacggcgc cgtgaacaaa gctcgggcgc gccgcatatc
     6481 ccgatcgtcg ccttgacggc caatgccatg gacggagacc gcgagcgctg cctcgcgcgc
     6541 ggcatggatg acttcctcag caagccgttc acgcggcagc agctggccac cctgctcagg
     6601 cgctggctgg cgttgcgcac cctccccgaa accgaacgcc acgatctgtc gcaggtgccg
     6661 ctcatcgacg ccggcgtgtt gcgcgacatc gccgccctcg gcaagccggc gctgctcgac
     6721 tcgatgatcg aattctacct gcaggagtcg ccgcgcttgc tcgcgacgat cgacagtgcg
     6781 gccgccgcta tgcgattcga tgccctgttg caggccgtcc attcgctcaa atccagcacg
     6841 gcgaatctcg gcggcagtcg actggcgttg gttgccaagg aatgcgagtc cctggtgcgc
     6901 gaaggcgcca tcgcgcaggt cgcgtcgttg gtgagccgga ttcacagcga ataccgggat
     6961 ttttgcgccg cgctcctcca agaacagtcg cgaagtgcag cgtaacggcg tgccaggctt
     7021 cgaacaaaca tgaacacgac cgactacaag ggtattgttc cagccaccgt gctcgtggtc
     7081 gacgacgatc ccggtgcgcg gctgttgctc gggaccgcat tggagatagc cggcttcgat
     7141 gtcatcaccg cggaggacgg gttggcggcg ttgcaccggt ttcgtgagcg tcccgcggac
     7201 tgcgtggtgc tcgatgtcgt gatgccggga atgaacggct tcgacgtgtg cagtgcgctg
     7261 cgtagacttc cgacctgcgg tcacgtcccc atcttgatgc aaaccagtct cgatgacatg
     7321 cagtcgatcg gtcgcgccta tgatgcaggc gccacggact tttcatccaa aggcatcaac
     7381 ccgatgttgc tcgcgcagcg cgtcaaattc ttggtgcgtg ccaaacagac tcaggaccag
     7441 ctgcgcgaga gtgaggcgcg ggttcgatat ctggcgtatt acgacccgct caccggattg
     7501 cccaatcgcc agcggttgct gcagattttg cagcagcaag tcgagtgggc cacgccgcgc
     7561 cgacgtggta tcgccgtgct gatgctcgac gtcgacaatt tctcgcggat caacgacacg
     7621 cagggtcagg cggcgggcga tgccctgctg aaagaagttg cccatcgcct gcagtattgc
     7681 ctgcgcgacg cgcagcgcat gctggaatcc gccgcggatt cgctcgatac caaggatatc
     7741 aacgattggg tggcgcgcac cggcggcgac gagtttgcgc tggccctgcc gggcgcggcc
     7801 accgccgaag ccgcgcaagt cgttgcgcgg cgtgtgcagg cggcattggc acgcccgttc
     7861 atctcggcgc tgcaggaatt cccgttatcc gcgagcatgg gcatcagcct gtttccgggc
     7921 gatgcaacga ctgccgagag tttggtcaag catgccgacg cggccatgca tcacggcaag
     7981 aagtcgggac gaggaggggt cgagttcttc aacaagtcga tcagcacgca ggcggcgcgg
     8041 cacctgtcga tggaggcgga tttgcgcaag gctctggagc gcggcgagtt caccttgcac
     8101 tatcaaccac ggctggcact gaaggatctg cgcgtcgagg cggtcgaggc tctgctgcgc
     8161 tggaagcatc cgcagcgcgg atccgtggcc ccggacgaat tcatcccgct ggccgagcaa
     8221 agcggattga tcacggaaat cggcgattgg gtgctgcgcg aagcctgtgc gcaggtgcgg
     8281 cgctggcgag atgccggtgc gcccgattgg caggtcgcgg tgaacgtctc cggtgtgcag
     8341 tttcgcgacg gcagcctggt gacgcgcgtg tcgcgcgcaa ttgatgccgc cgcaatcgat
     8401 tcgcggatgc tcgagctcga gttcaccgag ggtgcgttga tcgagtactc ggccgcggtc
     8461 agcaaggcgg taaagtcgct gaaggaattg ggcgtggcga ccgcgttgga tgacttcggc
     8521 accggctaca gttcgctgtc ctacctgcgg cattttccta tcgacacact caagatcgac
     8581 cgctccttcg tacgcgacat cgcctcgaaa accggcggca atgcgccgtt ggtcgacgcc
     8641 atcatcgcca tggcgaagag tctgggactt gcgaccgtgg ccgaaggcgt ggaatcggaa
     8701 gcccagtggc ggtatctgaa gacgcgggag gcgaaccagg tgcagggatt cctgttcgcc
     8761 cgtccgatgg caatcgcgga gttgcagcgc tggcatgccg aatggcttga taccaagcag
     8821 ttagccgctc tgagcgcagc ctagggtttg ttattcgtcc ggcgccagac gacttcactg
     8881 ccgttttcat gacgcgcgag cacgcgcgcc acgacgaaca atagatcgga caggcgattg
     8941 agatacttga gcgcctcggg cgacacggtc gcggtgcgcg ccagcgacca acagcggcgt
     9001 tccgcccgcc ggcacacggc gcgggcgacg tgacaggccg ccgccgccgg cccgcctccc
     9061 ggcaagatga actctttgag gggtggcaag ccgtcgttga atccatccaa ttggttctcc
     9121 aggcgatcga catgggccgc gccgatcatg cgatggcccg ggatgcacaa ttcgccgccg
     9181 agatcgaaaa gatcgtgctg cacttcaata agacaatgtg cgaccggctc cggcagcgcg
     9241 ccgacgccga gcacgacgcc gatcgcgctg ttcgcctcat cgacggtgcc gtaggcctcg
     9301 acgcgcaagt cctccttcgg cacgcgtgtt ccgtcgccca gaccggtgct gccgtcatcg
     9361 ccggtgcgag tataaatctt gctgagtcgg ttacccatcg ctcgaaaccc cctggcttgg
     9421 atgcgacggt agcacaaggt ttgctaaggt ggcgtccatg aagcattcgg ccgagctaca
     9481 ggctgctctc gacgcggcgg aacaagccgc cgctatcgcg cgcgcgatgt tcgaacataa
     9541 cgtcgaggta cgcatcaagg cggataagtc gccggtgacc gaggcggatg tacgctgtga
     9601 gatcgcgatt cgtgaaattc tggagcagcg cttcccgacg tacggcttct tcggcgagga
     9661 gaccgggtcg cgcgacacgc aggccgagag tctgtggctg gtggatccga tcgacggcac
     9721 caaggctttc gtgcgggagt atccgatgtt ctccacgcaa atcgcgctga tgcgtcgcgg
     9781 cgaattcgtt ctcggcgtgt cgagcgcccc cgtatatggc gaactctgtt atgccgagcg
     9841 tggtcgcggt acgttcctca aaggcaagcc cgcgcacgta agtcacgtgg caatgctcga
     9901 gtcggctgcg ctctccgccg gaaatgtgcg cagcctcgct atgggaagcg gttgggcgcg
     9961 ctacggagcc ttggtagcgc aggtcgatcg cattcgcggt tacggcgatt tcctacacta
    10021 tcacttgctc gccagcggca agatcgatgc ggtgatcgag agcgacgtga acattttgga
    10081 tattgcggcg tgcgcagcga tcgtcaccga ggccgggggc cgcttcacgg acttgaaggg
    10141 cgcagcgata tcgctggaat cgacctcggt gctcgccacc aacgggcgct tgcatgacgc
    10201 ggtgctcgct gccttaggtt aaattaccga tcagcggggt tgctgcggcc aacgtatttc
    10261 gagcgccgtg gcattgccga tcaccacctc gtccagactc accgcctgcc gccatttgtc
    10321 cggcgacaac acgcgccgcc cggccatcaa ttccgccgtc aattgcggat agcggggtac
    10381 ttgcgcggga tcgccgtagc cgtcgggaaa gcgcggcgcg tcgtcggcga ggttgtattt
    10441 gtcggtggca cccaccgtgt gcagcaattc gtgggcgatt accacatcgt tctcgccgtt
    10501 catttcgggg gccgcgaacg catacacgac tccgatcaat cccttcgtca gccccaatga
    10561 atgcgggacc gtcggcgtca acgccggatc atggtagagc acgaagatgc ggatgtcctc
    10621 gggttcatgc acgtgtccgc tcgcgcgcca ggcccagtat cgcaggcgca gactccaaaa
    10681 agcggtggcc agcaatccgg cgttcggttc gcgttgcggc gggcgatcct gcaattcggc
    10741 ccgaagccgg gttttgatcg gttcgctcgt atccagatgg tagcgcgcgg cctcgcgcgc
    10801 aaagaatcga tcgatgggct tgaagcgctc cgcatcgagc gccgtcacgt aggctcgcgt
    10861 caccgggctg tcgtccgccg caatgggata gatggcgacg tacagggggt tgtgccagcg
    10921 agtgctgaga tagcgatcct gccaggtagt gagcgcaacc acgaccaaca cggcgagcag
    10981 aatcgcgacg cgcagcgtct tccatccatt gcgggagggc gcggcgccgc cggaagacgc
    11041 cgtgccgcgc ttgggcggcg tgccccgcga gcggctcact cgccggactc gatgctgaat
    11101 tccgcgagtt ccccgtcgac cgagcggtag gagatcgaca tgggcttgca gcacacgggg
    11161 caatcctcga tatatgactg ttctggcacc gacaggtcga gtgtcaaatt gatggtttcc
    11221 caacaatgcg ggcaggcagc gtcttgatcc tgcagaaggt tcatcacgca atagtacccg
    11281 ggagccggcg gttgcggggg ttccaggcga ggcggcacag cgacacctcg aggcgctata
    11341 cttcgcactt ggcttgcggg gatcaatacg aggaatcatg gagccgaccg acacttcgaa
    11401 atccgactat ttccatcggg tggtcgattg ccaatgggca tgtccggcac acaccgatgt
    11461 acctgaatac attcgactga tcgcgcaggg cgaattcacc gaggcctaca tggtgaatcg
    11521 cgagtccaac gtgttccccg gcattctcgg ccgggtctgc gaccggccct gcgaacccgc
    11581 ttgccgccgc ggccgaatcg aggcaaagcc ggtggccatc tgccgcttga agcgcgtcgc
    11641 cgcggatcac cgagacgaca tttccgctct cttgcccaag gcgccgcgcg agaagaacgg
    11701 caagcgcgtc gcgtgcatcg gcgcaggacc cgcctcgctc acggtcgcca acgacctgtt
    11761 gccgatgggc tatgaagtga cgatattcga acaatacgcc gtgcccggcg gcttgatgcg
    11821 caccaatatc ccggcattcc gtcttccggc ggccgttctc gacgaggaga tcgggtatgt
    11881 cgtcgacatg gggg
//
LOCUS       DASWZT010000399         2233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_27351, whole genome shotgun sequence.
ACCESSION   DASWZT010000399 DASWZT010000000
VERSION     DASWZT010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27351"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /gene="purH"
                     /locus_tag="VGE92_11255"
     CDS             <1..1137
                     /gene="purH"
                     /locus_tag="VGE92_11255"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY1314448.1"
                     /translation="VVVDPHDYRELLDELAANQGATNVAMRQKLAAKAFAHTAQYDAM
                     VSAYFTGAIGGGVPTFPDDLNLSFRKHLDLRYGENPHQQAAFYTDPRAIGASVTRAKQ
                     IQGKELSYNNIADSDSALECVRQFDTAGCVIVKHANPCGAARAATLAEAYARAYRTDP
                     TSAYGGVIAFNRELDAATARAIVEQQFVEVIVAPSLSETAKALLTLKDNVRVLLVGDL
                     TLPVTQLLEYKSVAGGLLVQTRDTGVIMAQELRAVTERVPTLAELDDLIFAWRVAKYV
                     KSNAIVCAKDKATIGIGAGQMSRVVSSKIAAAKAQDAGLDMHGASIASDAFFPFRDGL
                     DAVAELGIKSVIQPGGSKRDADVIAAADEHGIAMVFTGMRHFRH"
     gene            1147..>2233
                     /gene="purD"
                     /locus_tag="VGE92_11260"
     CDS             1147..>2233
                     /gene="purD"
                     /locus_tag="VGE92_11260"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY1314449.1"
                     /translation="MKILIVGGGGREHALAWKCAQSPRVQQVFVAPGNAGTALEDKVR
                     NVDIPADDLAALLAFAAREDIGLTIVGPEGPLVAGIVDRFTAAGRACFGPSRAASRLE
                     GSKAFTKQFLERHSIPTASYKAFDHATFDAAYVRAQRLPLVVKADGLAAGKGVVICDT
                     HESAIDAANGMLGGSYGSAGDRIVIEEFLRGEEVSFIVIASDQAVVPLATSQDHKRRD
                     DGDRGPNTGGMGAYSPAPIVTQALHERIMHEVIEPTLRGLREDGNPYLGFLYAGLMIA
                     ADGTPNVLEFNCRFGDPEAQPILARLQSDLVDLCAAALDGTLTRVGPRWDPRAALGVV
                     MAAGGYPDSYRKGDAISGLDAAARLPGK"
BASE COUNT          425 a          719 c          700 g          389 t
ORIGIN      
        1 gtcgtcgtcg acccgcacga ctaccgcgaa ctgctcgacg aactcgccgc caaccagggc
       61 gcaacgaatg ttgccatgcg acagaagctc gccgccaaag ccttcgcgca taccgcgcaa
      121 tacgatgcca tggtgtcggc ctacttcacc ggcgcaatcg gcggcggtgt accgaccttc
      181 cccgacgacc tgaacttgtc gtttcgcaag cacctggatt tgcgctacgg cgaaaaccca
      241 caccaacagg ctgcgttcta taccgatcca cgtgccatcg gcgcctcggt gacgcgagcc
      301 aaacaaatcc agggcaagga actctcgtac aacaacatcg cagacagcga cagcgccctt
      361 gaatgtgtgc ggcagttcga cactgcgggc tgcgtcatcg tcaaacacgc caacccctgc
      421 ggcgctgcgc gagccgcgac actcgccgaa gcctacgcgc gcgcctaccg caccgatccc
      481 acctcggcgt atggcggcgt cattgccttc aaccgcgagt tggatgcggc gaccgcccgc
      541 gccatcgtcg aacagcagtt cgtcgaggta atcgtggcac ccagcctgtc cgaaacggca
      601 aaggcactgt tgacactcaa agacaatgta cgtgtgttgc tggttggcga tttgacgctg
      661 cccgtcacgc aactgctcga gtacaaaagc gttgccggtg gtctcctggt tcaaacgcgc
      721 gataccggcg tcatcatggc gcaggaactg cgcgccgtga cggagcgcgt gccgacactg
      781 gctgaactcg acgacttgat cttcgcatgg cgggtggcga aatatgttaa atccaacgcc
      841 atcgtctgcg ccaaggataa ggccaccatt ggcatcggtg ccgggcagat gagccgtgtg
      901 gtcagcagca aaatcgccgc tgccaaggcc caggatgcgg ggctcgatat gcacggcgcg
      961 agcatcgcat ccgatgcatt tttcccgttt cgggatgggc tcgatgcggt ggccgaactc
     1021 ggtatcaaat ccgtgatcca gccgggcggc tcgaaacgcg acgctgacgt catcgcggcg
     1081 gccgacgaac acggcattgc gatggtgttc accggaatgc gccattttcg ccactagggg
     1141 tcacgcatga agatcctgat tgtcggcggg ggcggccgcg aacatgcgct ggcatggaaa
     1201 tgcgcacagt cgccgcgcgt gcagcaggta ttcgtggcgc cgggcaacgc cggcaccgcg
     1261 ctggaagaca aggtccgaaa tgtcgatatc ccggccgacg atctcgcggc gctgctcgca
     1321 ttcgcggctc gcgaagacat cggactgacc atcgtcgggc cggagggtcc tctggtcgcc
     1381 ggcatcgtcg atcgattcac ggcggccggg cgcgcgtgct tcggcccgtc ccgtgctgcg
     1441 tctcgcctcg agggctcgaa ggcgttcacc aagcaattct tggagcggca ctcgatcccg
     1501 acggcgagct acaaagcctt cgaccatgca acgttcgatg ccgcgtatgt tcgtgctcag
     1561 cgattgccgt tggttgtcaa agccgacggt ctcgccgccg gcaagggcgt ggtgatctgc
     1621 gatacccatg agtcggcgat cgatgccgcc aacggcatgc tgggcggcag ttacggcagc
     1681 gccggtgatc gaatcgtgat cgaggaattc ctgcgcggcg aagaagtcag cttcatcgtg
     1741 atcgcgagcg atcaagccgt ggtgccgctc gccacctcgc aggatcacaa gcgacgcgat
     1801 gacggggacc gcggacccaa taccggcggc atgggtgcct actcgccagc gcccatcgtc
     1861 acccaggcgt tgcatgaacg catcatgcac gaggtcatcg aacccacgtt gcgcggtctg
     1921 cgcgaggacg gcaaccctta cctgggattt ctttatgccg ggctcatgat cgccgcggac
     1981 ggcactccca acgtgctcga gttcaactgt cggttcggcg atcccgaagc gcagccgatt
     2041 ctcgcgcggc tgcagagcga tcttgtcgat ctgtgcgcgg cagcgctcga cggcaccctg
     2101 actcgagtcg ggccgcgctg ggatccacgc gcagcgctcg gcgtggtgat ggcggccggc
     2161 ggctatccgg acagctatcg caaaggcgat gcgatcagcg gactcgatgc cgcggcacgc
     2221 ttgccgggca agg
//
LOCUS       DASWZT010000400         1648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_66017, whole genome shotgun sequence.
ACCESSION   DASWZT010000400 DASWZT010000000
VERSION     DASWZT010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66017"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..558)
                     /locus_tag="VGE92_11265"
     CDS             complement(31..558)
                     /locus_tag="VGE92_11265"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY1314450.1"
                     /translation="MKFKSTIVLAISALASSYAWAVAPLLTTTKTITIEAPASKVWNA
                     TRDFNGLATWHPALASDEIVEGKNNTVGAVRLLTLKGGGTIKEKLLAFNAAGRSFKYE
                     IVEGVLPVTEYTSTFTVKSAGKGKSTVVWTGHFKRKDVRDNPGEKDNDKTAVDTINGV
                     YQGGLDNLKKMLEAQ"
     gene            complement(582..>1648)
                     /locus_tag="VGE92_11270"
     CDS             complement(582..>1648)
                     /locus_tag="VGE92_11270"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1314451.1"
                     /translation="VIGSDLDSDDAVGAQIVEVLQRLPDSGNVRLAVPPREVELHLEL
                     LPAQLAVYGLQASDVLQTVNAAYHGSVAAELNQADRSVPVVVRVAHAASDPHALGALL
                     LRGRDGALTPLSAVAHISLVSARSLVDHQDGLRRQIVVASPKGADQSGYARAARQAIA
                     AKVTLPPGVFLRYGGAAEAQTAAKNELLLHSAAAFVLIVLLLALAFGHARHVLIVLLS
                     LPSTLIGGVAAVALTGSTLTLGAMVGFVALFGMAARNTILLVSHYDHLVHTENQPWNL
                     DTASRGAEERLTPVLLTALLTGLALLPVALQLHQPGHEIEGPMAVVILGGLVSSTLVS
                     LLLIPPLAARWLRPSMGIEV"
BASE COUNT          289 a          553 c          513 g          293 t
ORIGIN      
        1 gcgagagtcg ccgggccgcc cgcacgtcgc ctattgcgct tccagcattt tcttcagatt
       61 gtcgagcccg ccctggtaga ccccgttgat ggtgtcgacg gcggtcttgt cgttgtcctt
      121 ctcgcccgga ttgtcgcgca cgtctttgcg cttgaagtgt ccggtccaca ccaccgtcga
      181 tttgcctttg ccggcggact tcaccgtgaa ggtcgacgtg tattcggtca ccggcaacac
      241 gccctcgacg atctcgtatt tgaacgagcg cccggccgca ttgaacgcca gcagtttttc
      301 cttgatcgtt ccgccgccct tcaacgtcag caagcgcacg gcaccgaccg tgttgttctt
      361 gccctcgacg atctcatccg aggcgagcgc cgggtgccag gttgccaggc cgttgaagtc
      421 ccgggttgcg ttccacacct tgctcgcggg agcctcgatg gtgatggtct tggtggtggt
      481 gagcagcggt gccacggccc atgcgtagct tgatgccagc gcggaaatag ccaatacaat
      541 cgtacttttg aatttcattg ccttagagtc ctcgtgaaag attaaacctc aatccccatc
      601 gacggccgca gccagcgcgc cgccaacggc ggaatcaata acaggctgac gagcgtagaa
      661 gacaccaagc cgccgagaat taccactgcc atcggtcctt cgatctcatg tccgggctgg
      721 tgcagctgca atgccaccgg cagcagcgca agtcccgtga gcagcgccgt cagcagcacc
      781 ggggtcaggc gctcctcggc accccgcgag gccgtgtcca aattccaagg ctggttctcg
      841 gtgtgcacca ggtggtcata gtgcgagacc agcagaatcg tgttgcgcgc cgccatgcca
      901 aacagggcca cgaatccgac catggcgccg agcgtgaggg tgctgcccgt cagggcgacc
      961 gcggccacgc cgccgatcaa cgtgctcggc agggacagca gcacgatcag cacgtgacgg
     1021 gcatggccga atgccagcgc cagtaacaga acgatgagca cgaaggcggc cgcggagtgc
     1081 agcaacaact cgttcttggc cgcggtctgc gcttccgcgg cgccgccgta gcgcagaaac
     1141 acgccgggcg gcagcgtgac tttcgccgcg atcgcctggc gcgcggcccg tgcataaccg
     1201 ctttgatcgg cccccttggg cgaggccacc acgatttgac gccgcagtcc atcctggtgg
     1261 tcgaccagac tgcgcgccga caccaggctg atatgcgcca ccgcggacag cggcgtcagg
     1321 gcgccgtcgc ggccgcgcag cagcagcgca ccgagggcgt gggggtcgct ggccgcgtgg
     1381 gcaacgcgca ccaccaccgg cacactgcgg tccgcttgat tgagttcggc cgcgacgctg
     1441 ccgtggtagg ccgcattgac ggtctgcagc acatccgatg cctgcaggcc atatacggcc
     1501 aactgcgccg gcagcaattc caaatgcagc tccacttcgc gcggcgggac cgcgaggcgc
     1561 acgttgccgc tgtccggcag gcgctgcagt acttcgacga tctgcgcgcc gaccgcatcg
     1621 tcgctatcga gatccgagcc tattacgc
//
LOCUS       DASWZT010000401         8659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_56449, whole genome shotgun sequence.
ACCESSION   DASWZT010000401 DASWZT010000000
VERSION     DASWZT010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8659
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56449"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VGE92_11275"
     CDS             complement(<1..717)
                     /locus_tag="VGE92_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY1314452.1"
                     /translation="MARTTPIERYRNIGISAHIDAGKTTATERILFYTGVSHKIGEVH
                     DGAAVMDYMEQEQERGITITAAATTCFWKGMDKSFPEHRINIIDTPGHVDFTIEVERS
                     LRVLDSAVAVYCAVSGVQPQSETVWRQMNKYGVPRLAFVNKMDRAGANFLRCVEQIQT
                     RLRGNPVPIQLPIGAEDGFVGVVDLVKMKSIWWDEETQGMKFEYRDIPAEMLKECEQW
                     RAKMIEAAAEGNEALLNKYLE"
     gene            complement(832..1311)
                     /gene="rpsG"
                     /locus_tag="VGE92_11280"
     CDS             complement(832..1311)
                     /gene="rpsG"
                     /locus_tag="VGE92_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003369032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEY1314453.1"
                     /translation="MSRRAAAPHRTILPDPKFGNDMLAKFINMVMERGKKSAAEKIVY
                     GAIDRIGDKSGRAGGEAIAVLQQALDNVKPVVEVKSRRVGGATYQVPVEVRANRRQTL
                     AMRWVIEAATARSEKSMAHRLAAELMEAAENRGAAVRKREDTHRMAEANKAFAHYRW"
     gene            complement(1321..1695)
                     /gene="rpsL"
                     /locus_tag="VGE92_11285"
     CDS             complement(1321..1695)
                     /gene="rpsL"
                     /locus_tag="VGE92_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEY1314454.1"
                     /translation="MATTTQLIRTGRNEPAWKSKVPALASSPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGYEVSSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGAL
                     DCAGVSDRRQGRSKYGAKRPKK"
     gene            complement(1827..2396)
                     /locus_tag="VGE92_11290"
     CDS             complement(1827..2396)
                     /locus_tag="VGE92_11290"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEY1314455.1"
                     /translation="MRVARRAVVLIGLLAAWAVAGVALAENPTVGEPAPDFRAVTVGG
                     TRLSLADFKGQVLVIKFWPPGCTPCKTELPILDSYYQLQKKVGLRVIAVPPANTPRWH
                     QLQRAASALAILVIREFRGGYMALTGHPTNYVIDRAGILRYADSGAWTLEDLNRILVP
                     LLREGVPVPEEQIQGHPALAMRAAEQQLP"
     gene            2487..2807
                     /locus_tag="VGE92_11295"
     CDS             2487..2807
                     /locus_tag="VGE92_11295"
                     /inference="COORDINATES: protein motif:HMM:NF016245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HEY1314456.1"
                     /translation="MPGNGHLKYGHSWPLWQAILVSWLIALFEYCLAVPANRLGFGQF
                     TGFQLKILQEAITVAVFIVFAITFLKEKMAWNYAVSFALLIAAVVVAFARLGPETGST
                     PPGT"
     gene            2812..3846
                     /locus_tag="VGE92_11300"
     CDS             2812..3846
                     /locus_tag="VGE92_11300"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEY1314457.1"
                     /translation="MITVFSPDHVLRSARTELYGGELVRPHESAERAQIVLQQVQAQR
                     LGEVVEPARFGAAPRLRVHDAGYLAFLETAWSEWVAAGNRGEAIPDCWPARRMAQRCP
                     ADISGKLGYYAMAGETSISGGTWEAACAAADVALSAASYIKDGARGAFALCRPPGHHA
                     AHDLYGGYCFLNNAAIAAQFLRDAGAARVAILDVDFHHGNGTQDIFYDRSDVLYVSLH
                     CDPAHAFPYFSGYADETGAGRGTGFNLNLPLPAGTEFSAWQAALLTALKRIADFRADA
                     LIVSLGVDTFAEDPISSFRLQSADFLTYGQLLAACGLPTLFVLEGGYAVAAIGVNVVN
                     VLMGFEVPGA"
     gene            3918..4220
                     /locus_tag="VGE92_11305"
     CDS             3918..4220
                     /locus_tag="VGE92_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314458.1"
                     /translation="MKALAWRAALNIIQSVTGTADGQTITGLANYGGADNYLFPPTTT
                     TPFSYGGFAFTTSGESFNLFSDLDPGPILGGSHGVDECNSIASPSCTYTSGSVSVA"
     gene            4310..4486
                     /locus_tag="VGE92_11310"
     CDS             4310..4486
                     /locus_tag="VGE92_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314459.1"
                     /translation="MSTLTQPNYGLRVLPHVDAAFDPDPMTIADGLYAPRFLQDPCFR
                     CVSQPRLNWAGWSP"
     gene            complement(4574..4948)
                     /locus_tag="VGE92_11315"
     CDS             complement(4574..4948)
                     /locus_tag="VGE92_11315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEY1314460.1"
                     /translation="MATITKQKQKARTMHSPSHPGEILRELYLKPMRVSITEAAAALG
                     VSRKHFSAIVNGRAPVTPDMAMRLAGAFETEAELWVNLQAQHDLWVVSQKGETGPTSL
                     PLPSSGLPGTPARAGSGCVSSP"
     gene            complement(5022..5702)
                     /locus_tag="VGE92_11320"
     CDS             complement(5022..5702)
                     /locus_tag="VGE92_11320"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY1314461.1"
                     /translation="MKDGPNIARIAALLGDPARADVLTALMTDRALTATELAAIAGVT
                     KQTMSAHLAKLLGAGLVDMEQQGRHRYFRLADADVAGLLESLMGVAFRTGAVRLIASP
                     REPALRKARICYDHLAGELGVAAYEALLRHNAFEMSHNELRLSKSGAAWFSRLGIDAQ
                     AAARQRRTFCRPCMDWSERRHHLAGSLGAALLSRLQELKWAARDRNSRVMKFTPRGER
                     AFLDAVRA"
     gene            5755..6090
                     /locus_tag="VGE92_11325"
     CDS             5755..6090
                     /locus_tag="VGE92_11325"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEY1314462.1"
                     /translation="MSIVCFIRYQIDPWQRDAFRSYAENWSRIIPRCGGHLIGYFLPH
                     EGTNDIASGLIKLDSLAAYESYRSRLKQDPAARDNFAFAQARRFILREERTFLEVVDA
                     AFALPALPP"
     gene            complement(6123..6560)
                     /locus_tag="VGE92_11330"
     CDS             complement(6123..6560)
                     /locus_tag="VGE92_11330"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY1314463.1"
                     /translation="MPGLMIGELARQAGVTTSALRYYEKAGLLAAPARTSNRRQYDRA
                     VLGRVRIILLARDAGFSISETRMFLNGFPIGTKPALRWRAMAERKIAELDALSTRLGR
                     MRSILNASFHCECRRLEECERLIAAKTCCGPESKFPRVSGKIG"
     gene            6673..7446
                     /locus_tag="VGE92_11335"
     CDS             6673..7446
                     /locus_tag="VGE92_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314464.1"
                     /translation="MQIEKRWYLTALLSLCVCSIGRADSVPLTPVTVAAAISDPRRPD
                     DQVKLDVARRPAISLLFSGARAGDRIADVMSGNGYFTRILSDVVGPSGHVYAFIPTEQ
                     IAHCSPREIAGTQAIVRDSSFRNVTLLTGSLADFHLPQKLDLIWMSQSYHDLHDSFLG
                     PANVALFNKALFDALQPGGVLLVIDHVAAAGSGLRDTETLHRIDPIRMQNEIEAAGFV
                     LESQSDALRNTDDDHKRAIFDPSIRGRTDQVLFRFRKPQ"
     gene            complement(7469..>8659)
                     /gene="hutI"
                     /locus_tag="VGE92_11340"
     CDS             complement(7469..>8659)
                     /gene="hutI"
                     /locus_tag="VGE92_11340"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEY1314465.1"
                     /translation="PYGNIKNAALAVKGERIAWLGSAAEGQTRAATQGLPVEDARGLW
                     ITPGLIDCHTHLVYGGNRVEEFEKRLCGVSYEDIARAGGGIQSTVQATRAASHEELYR
                     EAAVRAQRLMSEGVTTIEVKSGYGLELAAERRLLEVARELGSELPVSIKKTFLGLHAL
                     PPGADRQQFVSEVCGPWLESLAAAGLVDAVDAFCENIAFTVAETERLLRAAKQLGLPA
                     HVHAGQLSDMGGAELAAKWGALSADHLEYLSAAGARSMAAAGTVAVLLPTAYFTLRQT
                     TPPPVALLRESGVAVAVATDSNPGSSPCTSILLALNMACTLFGLTPEEALSGATRQAA
                     RALGAAAEIGTIEVGKRADLALWRIERPAELCYGIGANPCVAVMHRGQLRTGGVRSDH
                     GRAS"
BASE COUNT         1566 a         2827 c         2546 g         1720 t
ORIGIN      
        1 ctccaggtac ttgttgagca gcgcctcgtt gccctcggcg gcggcctcga tcattttggc
       61 gcgccactgt tcgcactctt tgagcatctc cgccgggatg tcgcggtact cgaacttcat
      121 gccctgagtt tcctcgtccc accagatcga cttcatcttc acgagatcga ccaccccgac
      181 gaatccgtcc tcggcgccga tcggcagctg aatggggact ggattgccgc gcagccgtgt
      241 ttgaatctgc tcgacgcaac gcaggaaatt cgcgccggcg cggtccatct tgttgacgaa
      301 cgccagccgc ggcacgccgt acttgttcat ctgacgccac accgtctcgg actgcggctg
      361 cacgccggag acggcgcaat acaccgccac ggcggaatcg agcacgcgca agctgcgctc
      421 cacttcaatg gtgaaatcga cgtgccccgg cgtatcgatg atgttgatgc ggtgctcggg
      481 gaagctcttg tccatcccct tccagaagca cgtggtcgcc gcggcggtga tggtaatgcc
      541 gcgctcctgc tcctgttcca tgtagtccat caccgcagcg ccgtcatgca cctcgccgat
      601 tttgtgcgac acgccggtgt agaacaaaat acgctccgtc gcggtggttt tccccgcgtc
      661 gatgtgagcg gaaataccga tgttgcggta tcgctcgatg ggcgtagtgc gtgccataaa
      721 tactttccag gcgcttcgcg ccgaattcaa cggggtcgat gcgaccccgt ccgaggaatc
      781 aatatatggc cgaaccggcg caaatccagc cggtagtcgg cgtccttcgc gctaccagcg
      841 gtagtgcgcg aacgctttgt tggcctcggc catgcgatgt gtgtcttcgc gcttgcgaac
      901 cgcggcgccg cgattctcgg cagcctccat cagttcggct gcgagccggt gcgccatgga
      961 cttctcgctg cgcgccgtgg ccgcttcgat gacccagcgc atcgcgagcg tttgacgccg
     1021 attggcgcga acttcgacgg gcacctggta agtggcgccg ccgacgcgac gggatttgac
     1081 ctcgaccact ggcttgacgt tatccaacgc ctgctgcagc acagcaatgg cttcgccgcc
     1141 ggcgcgtccg ctcttgtcgc cgatgcgatc gatggcgcca tagacgatct tttcggcggc
     1201 ggactttttg ccgcgctcca tgaccatgtt gatgaatttg gccaacatat cgtttccgaa
     1261 cttgggatcc ggaaggatgg tgcgatgcgg tgctgctgct ctgcgggaca tggaaaaccc
     1321 ttatttcttg ggacgcttgg cgccgtactt ggaacggcct tggcgacgat cgctgacgcc
     1381 ggcacaatcg agggcgccgc gcaccgtgtg atagcggact ccgggcagat ccttgacgcg
     1441 gccgccgcgg atcaacacca ccgagtgctc ttgtaagttg tgaccttcgc cgccgatgta
     1501 gctcgatact tcgtagccat tggtgaggcg cactcggcaa accttgcgca gcgccgaatt
     1561 cggctttttc ggtgtggtcg tgtacacccg ggtgcagacg ccgcgctttt gcggcgaaga
     1621 cgcgagcgcg ggaaccttgg atttccaggc gggttcgttg cggccggtgc gaatgagttg
     1681 ggtcgtcgtc gccataaata cctttagagc cgcagctaca cttgaagagc ggcccgatgc
     1741 atggacaaaa ttttaatgcg cggtaatgtc ccgcacttgc gcccggagac aaaggccggc
     1801 gattgtagga accggccgcg cgagtgtcaa ggtaattgct gctctgcagc acgcattgcc
     1861 agcgccgggt gcccctgtat ttgctcctcg ggcaccggca ctccctcgcg cagcagcgga
     1921 accagaattc ggttcaaatc ctcgagggtc catgcgccgg agtccgcata gcgcaatatt
     1981 ccggcgcgat cgatcacgta gttggtcggg tggcccgtga gggccatgta gccgccccga
     2041 aattcccgta tcaccaagat ggccaatgcg gacgccgcgc gctgcaattg gtgccatcga
     2101 ggggtattcg ccggcggaac ggcgatcacc cgcaggccta ctttcttctg cagctgatag
     2161 taggaatcca agatcggcaa ctctgttttg cagggcgtgc acccgggcgg ccagaatttg
     2221 atcaccaaga cctggccctt gaaatccgcc agcgacaatc tcgtcccgcc caccgtgacg
     2281 gcgcggaagt ccggcgccgg ttcgccgacc gtgggatttt cggccagggc aaccccggca
     2341 accgcccacg cggccaacag cccaatcaaa accacggctc gacgtgccac cctcataata
     2401 acccgcgtcc cagtgatcaa cctttgcgcc gcggcctacc catgacgacc atcattttgc
     2461 tgatctgttc caacgtgttc tgacctttgc ccggtaacgg gcacctcaaa tacggtcact
     2521 cctggccgtt gtggcaggcc attctggtgt cctggttgat cgcgttgttc gaatattgcc
     2581 tggcggtgcc ggccaatcgc ttgggattcg ggcagttcac gggctttcag ctcaaaattc
     2641 ttcaggaagc gattacggtg gcggttttta ttgttttcgc catcacgttc ttgaaggaaa
     2701 aaatggcctg gaattacgcc gtgtcttttg cgctgctgat cgccgcggtc gtggtagcgt
     2761 tcgccaggct tggcccagag acaggatcca ctccaccagg gacttaagat catgattact
     2821 gttttttcgc cggatcacgt gctgcgcagt gcgcgcacgg aactctatgg cggcgagttg
     2881 gttcgcccgc acgaatctgc cgagcgcgcg cagatcgtat tgcagcaggt gcaggcacag
     2941 cgtctcggtg aagtcgtcga accggcgcgc tttggtgcgg cgccgaggct ccgcgtccac
     3001 gatgccggat acctcgcatt tctcgagacc gcctggagcg aatgggtcgc cgccggcaat
     3061 cgcggcgagg ccatacccga ctgctggccc gcgcggcgca tggcgcaacg ctgtccggcg
     3121 gacatctcgg gcaaactggg ttactacgcc atggcgggcg agacatcgat cagcggcggc
     3181 acttgggagg ccgcgtgcgc cgcggccgat gtcgcgctga gcgcagcctc gtacatcaag
     3241 gacggcgccc gcggcgcctt tgcattgtgc cgtcccccgg gacaccacgc cgcgcacgat
     3301 ctgtacggcg gttactgctt cttgaacaac gccgcgatcg cagcccagtt cctgcgcgac
     3361 gcgggagcgg cgcgcgtcgc catcctcgac gtcgattttc atcacggcaa cggcacccag
     3421 gatattttct acgatcgctc ggatgtgctg tacgtgtcgc tgcactgcga tcctgcgcat
     3481 gcctttccct atttttccgg ttacgccgat gagaccggcg ccggtcgcgg caccggcttc
     3541 aacttgaatc ttcccctgcc cgcgggaacg gaattcagcg cctggcaggc ggcgctgctg
     3601 accgcgctca agcgtatcgc ggattttcgc gccgatgcgc tgatcgtttc tctgggcgtg
     3661 gacacctttg ccgaagatcc catcagttct tttcgtctgc agtccgcgga tttcttgacc
     3721 tatgggcaac tattggctgc ctgcgggttg ccgacgctgt tcgtgctcga agggggctac
     3781 gcagtcgcag ccatcggcgt caatgtggtg aacgtgttga tgggcttcga ggtgcccggc
     3841 gcttaaaaat aacctgcgat ttgatttgaa aacgcctaac ataatctgag aacaaatccg
     3901 ttggtcagga ggacgcaatg aaagcattag cctggcgggc ggctcttaat attatccaaa
     3961 gtgtcacggg cacggcggac ggccagacca tcaccggttt ggcgaattac ggcggggccg
     4021 acaattattt gttccctccc acgacgacga cgccgttcag ctacggtggc ttcgcgttta
     4081 cgaccagcgg agagtccttc aatctcttca gtgacctcga cccgggcccg atattgggcg
     4141 gcagtcacgg tgtagacgag tgcaacagca tcgccagccc aagctgcact tataccagcg
     4201 gctccgtcag cgtagcctag gaggatgcat gtcttcgagg gttgttttca caacaggcac
     4261 gttgctcatt gcggcttcgc tcctgagtgc gtgcgcccca agcatttcaa tgtcaacttt
     4321 gactcagcct aactatggac tgcgcgtgct gccgcacgtc gacgcggcgt tcgatccgga
     4381 tccgatgacc atcgcggacg ggctatacgc accccgattc ctgcaagacc cgtgctttcg
     4441 ctgcgtctcg cagcctcgtt tgaattgggc cggctggtcg ccttaacgag ttcgtgttcc
     4501 gcgacatcac gccctgaccg cgcctgcgca ccgcgctccc atgcatcgaa cgaatcgtcg
     4561 agttgctcgc gatctacggc gagctcacgc agccgctccc agccctcgcg ggtgtaccag
     4621 gcaagcccga gctcggcagt ggcagcgatg tcgggcctgt ctcgcctttc tgactcacca
     4681 cccacaggtc gtgctgtgcc tgcaggttca cccagagttc agcctcggtc tcgaacgccc
     4741 ccgccagccg catggccatg tcgggcgtca ccggcgcccg gccgttgacg atagccgaga
     4801 agtgcttgcg gctgacaccc agtgccgcgg ccgcctcggt gatcgagacg cgcatcggct
     4861 tcagataaag ctcgcgtagg atttccccgg gatgcgacgg actgtgcatg gtccgcgcct
     4921 tttgcttctg cttagtgata gtcgccataa tcctcctcca gcgcgtgctt tccctcgaat
     4981 cgaaaaatca cgcgaaagtt ctcatcgacc cacaccaagc atcacgccct gaccgcgtcc
     5041 aggaacgccc gttcgcctcg cggtgtgaac ttcatgaccc gcgaattgcg gtcgcgtgcc
     5101 gcccacttga gttcctgaag ccgcgacagc agcgccgcgc ccaatgagcc cgccagatga
     5161 tgccggcgct cgctccaatc catgcaaggc cggcagaagg tgcgccgctg tcgggccgcg
     5221 gcctgcgcgt cgatgcctaa acggctgaac cacgcggcac ccgacttgct caagcgtaac
     5281 tcgttgtgcg acatctcgaa ggcgttgtga cgcagcagcg cctcataggc ggcaaccccc
     5341 agctcacccg ccagatggtc gtaacaaatt ctcgccttgc gcagcgccgg ctcgcgcgga
     5401 cttgcgatca gccgcacggc accggtgcga aaagcgaccc ccatcaacga ctcgagcagc
     5461 cctgccacat cggcatccgc aagccggaaa tagcggtgcc ggccctgttg ttccatgtcc
     5521 acgagcccgg cgccgagcag cttcgccaaa tgcgcgctca tggtctgctt ggtgacgccg
     5581 gcaatggccg ccaattcggt cgcggtcagt gcgcggtcgg tcatcagggc tgtgagcacg
     5641 tcggcgcgcg cgggatcacc gagcagcgcg gcaatgcgcg cgatattcgg tccgtctttc
     5701 atggttcgac gatagacgaa ccatggatcg tccgcaacgg cttaccatcc gcgcatgagc
     5761 atcgtgtgtt tcattcgcta ccagatcgat ccctggcagc gcgacgcgtt ccgatcgtat
     5821 gcggaaaact ggagccgcat cattccccgt tgcggcggcc acctcatcgg ctattttcta
     5881 ccgcacgagg gcacgaacga tatcgcttcg ggtctcatca agctcgactc gctcgccgcg
     5941 tacgagtcct atcggtcgcg gttgaaacaa gatcccgctg cgcgtgacaa ctttgcattc
     6001 gcgcaagccc ggcgttttat cttgcgcgaa gagcgcacct tcctcgaagt ggtcgacgcc
     6061 gcctttgcgt tacccgctct gccgccgtag gcggccgacg cgcggcacgg tcgaagacga
     6121 tcctagccaa tttttccgct cactctcgga aacttggatt cgggaccaca gcaagtcttg
     6181 gcggcaatca gtcgctcaca ctcttcgagc cggcggcact cgcagtgaaa actggcattg
     6241 aggatggatc gcatccggcc aaggcgcgtg ctcagcgcat cgagctctgc gatcttgcgc
     6301 tcggccatcg cgcgccatcg caacgccggt ttagtgccaa tggggaagcc gttcaagaac
     6361 atgcgcgtct cgctgatcga aaaacccgca tcgcgggcca gaagaataat ccgcacccga
     6421 ccgagtacag cgcgatcgta ttgtcgacga ttggacgtgc gtgccggcgc cgccagcaat
     6481 ccggctttct cgtaatagcg cagcgcagaa gttgtcacgc ccgcttggcg tgccagctcg
     6541 ccgatcatga ggcctggcat cgaaacctcc tcgacatgcc cgaaagactg cttgacttaa
     6601 agcgcacttt aacttgtagg ctgcggcaaa ggtagaccga tgggcgactt aaggtcacgc
     6661 aaggagccgc gtatgcaaat cgaaaagcgc tggtatttga ctgctctcct gagcttatgc
     6721 gtatgcagca tagggcgggc ggacagcgtg ccgctcacgc ccgttaccgt cgcggcggcc
     6781 atctcggacc cgcgcagacc tgatgatcag gtcaagctcg acgtggcccg caggccggcc
     6841 atttcgctgc tgttttccgg agcgagagcg ggtgaccgaa ttgcggatgt gatgtcagga
     6901 aatggctact tcacgcgaat tctgagcgac gtcgtgggcc caagcggcca tgtttatgca
     6961 ttcattccga ccgagcagat cgcccattgt tcacctcgcg aaatcgcagg cacgcaagca
     7021 atcgttcgcg actccagttt tcgaaacgta accttgctca cgggttcact cgccgacttt
     7081 cacctgcccc agaaacttga tttgatatgg atgtcgcaga gctatcacga tctgcacgac
     7141 tcctttctcg gaccggcgaa tgttgcgctc ttcaacaagg ccctcttcga cgcgttgcag
     7201 cccggcggag ttcttctggt catcgaccat gtcgccgccg ccggctccgg acttcgggat
     7261 acggaaacac tgcatcggat tgatcccatc cgaatgcaga acgagatcga agcggcaggt
     7321 ttcgtgctcg aatcgcaaag tgacgcgctg cgtaataccg acgatgatca caaacgcgcc
     7381 atcttcgatc cgagcatccg cggcaggacc gatcaagtgc tgttccgatt ccgcaagccc
     7441 caatgacgcg ccgcagcggc gcctatgctc agctcgctcg gccgtggtct gagcgcacgc
     7501 cgcccgttct aagctgtccg cgatgcatca cggcaacgca ggggttggcg ccgatgccgt
     7561 agcagagctc cgcgggccgc tcgattcgcc acagagccaa atccgcgcgc ttgccgactt
     7621 cgatggtgcc aatttccgcc gcggcgccca aggcgcgcgc ggcctggcgc gtcgcgccac
     7681 tcaacgcctc ctccggagtc agcccgaaca gcgtgcaggc catgttgagc gccagcaaaa
     7741 tcgaggtgca cggcgaactc cccggattgc tgtcggtcgc gacggcaact gccacacccg
     7801 actcccgcag caacgccacc ggcggcgggg tcgtttgccg cagcgtgaag tacgccgtgg
     7861 gcaacagcac cgcaaccgtg ccggcagcgg ccatcgatcg cgcgcccgca gcgctcaagt
     7921 actcgagatg gtccgccgac agcgcgcccc acttcgcagc caattccgcc ccgcccatgt
     7981 cgctcaattg accggcgtgc acatgcgcag gcaatcccag ctgcttggcg gcgcgcagca
     8041 gccgctccgt ttctgccacc gtaaaggcta tgttctcgca gaaggcatcg acagcgtcca
     8101 ccaggccggc ggcggcgagt gactcgagcc acgggccgca gacctcgctc acgaactgtt
     8161 gccgatcggc ccccggcggc agcgcatgca gacccagaaa cgtcttcttg atcgagaccg
     8221 gcagttcgct cccgagctcg cgcgccactt ccagcaagcg gcgctcggcc gcaagctcga
     8281 gcccgtagcc cgacttcact tcgatggtgg tgacgccctc cgacatcaag cgctgggcgc
     8341 gaacggcggc ctctcgatac agctcctcat gactcgcggc ccgcgtcgcc tgcacggtcg
     8401 attggatgcc gccgccggcg cgcgcgatat cctcatagga aacaccgcac agccgctttt
     8461 caaattcctc gacccgattg ccgccgtaca ccaggtgcgt atgacaatcg atcagtcccg
     8521 gggtaatcca cagtccccgc gcatcctcaa ccgggaggcc ttgcgtggcc gcccgtgttt
     8581 gaccctccgc ggccgaacct agccacgcga tgcgctcgcc cttcaccgcc agcgcggcat
     8641 ttttgatatt gccatacgg
//
LOCUS       DASWZT010000402         6039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_42081, whole genome shotgun sequence.
ACCESSION   DASWZT010000402 DASWZT010000000
VERSION     DASWZT010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6039
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42081"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2317)
                     /gene="lon"
                     /locus_tag="VGE92_11345"
     CDS             complement(<1..2317)
                     /gene="lon"
                     /locus_tag="VGE92_11345"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEY1314466.1"
                     /translation="MNAEDVLIILPVANAVLFPGVVLPVAVSGDAALAAAQEAVRTQR
                     RVGLVLQTDTTGAAPGPDNLHRVGTAATIVRFITAPDGTHHLIAQGEERFTVLDFVSR
                     EPFLVARIEAHKEATVFDREVEARGLNLRDKALEAVQLLPQAPGELVNAIRSIESIPA
                     LTDIVASFMDLKTADKQDLLATFDLKTRLDRVLALLGRRIEVLKVSRQIDERTREAFD
                     ERQKEAVLRERLRQIQKELGETDNSPAELEELKQAIKDAGMPPDAQEQAQRELKRLER
                     MPDSAAEYSMTRTYLDWLLAMPWSKVDPETVDIDQARKILDQDHYGLDKVKRRILEFL
                     AVRKLNPEGRSPILCFVGPPGVGKTSLGQSIAKAIGLKFGRVSLGGVHDEAEIRGHRR
                     TYIGSLPGNIAQAVRKAGRRNPVLMLDEVDKLSASHQGDPFSALLEVLDPEQNNSFRD
                     NYLAVPFDLSKVLFIGTANVLDAIPAPLRDRMEVIELTGYSEEEKLQIATRYLLPRQL
                     QANGLTSQQVEISEEALRRLIIDYTREAGVRSLERQIGAVLRNAAVTIASGGEPKIVI
                     GADEVPRILGAARFESDVALRTSVPGVATGLAWTPAGGDILFIESSRVRGSGKLILTG
                     QLGEVMKESAQAAVTWVKSQADRMRIDPAALDGSDLHIHVPAGAIPKDGPSAGVAIAT
                     SLASLLSQRTVRADVAMTGEISLRGVVMPVGGIKEKCVAAARAGIRTVILPARNRRDL
                     EDIPESVRAKLEFVWAEKIDDVLARALEDPAE"
     gene            complement(2314..2724)
                     /locus_tag="VGE92_11350"
     CDS             complement(2314..2724)
                     /locus_tag="VGE92_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY1314467.1"
                     /translation="MNTHRRKSWMWAEACALLDQAERKHRHFFELLAAPAAAPAWEPP
                     ADIFTQGSELQVVVALPGARADEVVVQITPNGLQIDTVVPPPALDVHASVVRLEIPYG
                     RMRRRIDLPAGHYVLIERRLDRGCLYLRLTRQAP"
     gene            complement(2782..3618)
                     /locus_tag="VGE92_11355"
     CDS             complement(2782..3618)
                     /locus_tag="VGE92_11355"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEY1314468.1"
                     /translation="MTTRTSDATMAVFLDLENIALGARDAKYPSFDIEKVLARLLLKG
                     HIVVKKAYCDFDRYKDFKRGLHEAAFELIEIPHVRQSGKNSADIRMVVDALDLCYTKG
                     HVDTFVILSGDSDFSPLVSKLRENAKTVIGVGVKNSTSDLFINNCDEFIYYDDLVRKE
                     PSKARRRPPAARAPAPAEAAPGENKGPDLHDALELIVDTLDALGEERGGSEPIWGSMI
                     KQAIKRRHPGFNERAYGFKSFNDLLADAEKRGLLALQADEKSGGYTVRIPDRPAPRAS
                     SD"
     gene            complement(3639..4256)
                     /locus_tag="VGE92_11360"
     CDS             complement(3639..4256)
                     /locus_tag="VGE92_11360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HEY1314469.1"
                     /translation="MTIHRLFPTLVYADALQKRGSSEFNRQLLKECRQLRHDDAAGRR
                     WSAANYPQGYTSYGSVHWLQRISPTFKTLESKLARHVAAFARAVEWDLDGRELKMTDC
                     WINIMAKHVVHGLHLHPLSTLSGTYYVRVPAGAPGIKFEDPRLDRFMAAPPRKSNGKR
                     ETKPWGTFPARAGRLVLFESWLRHEVVPNRIAAERVSISFNYNWF"
     gene            4333..5661
                     /locus_tag="VGE92_11365"
     CDS             4333..5661
                     /locus_tag="VGE92_11365"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1314470.1"
                     /translation="MNFAPTAPLQQSGTAIPWRVVPPLVSTALLMFGSGFLGSLLPLR
                     FSAMGMSDGVIGLIGTCEALGFLVGCLYAHRLIAPVGLDRAYATFASFKSVAILGLCF
                     AQSVPVLSLLRFMIGVNAAGLAVIVESWLNALVPNEQRGRVLTIYVLVYGLFFGVGQL
                     FSRGFDVKGPEILIIAGMATTLALVPMVAIDVRAPALPHRVKLEILKALRTSPVSVTA
                     CLLNGLILTAFTTVGPLIGVRIGFDQQRIVLLMACVSLGGLFLQWPIGYFSDKIDRLH
                     ALIGLGLGILAVAAALMWADQRMPLLVLALLFGIFGGLGESLYAVGVAHANDRAVAAD
                     YVALSSTLLFVWALGSALGPTIGTLAIQLVAPGAFFAYVIVLTLIFTVFAIWRLSRRK
                     GDRIVESREEFLAYPQTSPEIYAWLPYHKDAAHTAPPITADAAPDAAKPP"
     gene            5685..>6039
                     /locus_tag="VGE92_11370"
     CDS             5685..>6039
                     /locus_tag="VGE92_11370"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEY1314471.1"
                     /translation="MKTPVLVLLAIAGWAAVPVRAAAGYTPITPSMSDRTVTLDGHNL
                     SIDEIVMVARYGAKVQLKPEARQREADNYGLLLEAAAEGIPVYWFNRGAGDQRETVMF
                     DGDPMSPKNHALIAKT"
BASE COUNT         1015 a         1898 c         1923 g         1203 t
ORIGIN      
        1 gctcggctgg gtcttccagc gcgcgggcga gcacgtcgtc gatcttctcc gcccagacga
       61 actcgagctt cgcgcgcacg ctctcgggta tgtcctccag gtcgcgccgg tttcgagccg
      121 gcaggatcac ggtacgaata ccggcgcgcg cagcggccac gcacttctcc ttgatgccgc
      181 ccaccggcat caccacgccg cgcaagctga tctccccggt cattgcgacg tcggcccgca
      241 cggtgcgttg cgacaggagc gatgccagtg acgtggcgat ggcaacgccc gcgctcggac
      301 catccttcgg gatcgcgccc gccggcacgt gaatgtgcag gtcgctgcca tccaatgcgg
      361 caggatcgat gcgcattcga tcggcctggg atttcaccca ggtgaccgcg gcctgagcgc
      421 tctccttcat cacctcgcca agctgaccgg tgagaatgag tttgccgctg ccgcgcacgc
      481 ggctcgactc gatgaagaga atgtcgccgc ccgccggcgt ccaggcgagg ccggtggcga
      541 ctccggggac gctggtgcgc aaggccacat cgctttcaaa acgcgccgca cccagaatgc
      601 gcggtacctc gtccgcaccg atgacgatct tcggttcacc accggaggcg atggttacgg
      661 cggcattgcg caacaccgcg ccgatctggc gttccaggct gcgcacgccg gcctcgcgcg
      721 tgtaatcgat gatcaggcgg cgcagcgcct cctctgaaat ttccacctgc tgcgacgtga
      781 gtccgttcgc ctgcaattgc cgcggcagca gatagcgcgt cgcgatttgc agcttctctt
      841 cttcgctgta gccggtgagt tcgatgactt ccatacgatc gcgcagcggc gccggtattg
      901 cgtccaaaac attggcggtg ccgatgaaca gaaccttgga caggtcgaac ggcactgcga
      961 ggtaattgtc gcggaacgag ttgttttgtt ccggatcgag tacctcgagc aaagccgaaa
     1021 acggatctcc ctgatggctg gccgagagtt tatcgacctc gtcgagcatc agtaccggat
     1081 tgcggcggcc ggctttgcgc accgcttgcg cgatgttgcc gggcagcgac ccgatgtagg
     1141 tgcgccgatg gccgcggatc tccgcctcat cgtgcacgcc gcctaagctt acgcgtccga
     1201 acttgagacc gatcgccttg gcgatgctct ggccgagcga ggtcttgccg acgcccggcg
     1261 gcccgacgaa acacaggatg ggactgcggc cctcgggatt gagcttgcgc acggccagga
     1321 actccaagat gcggcgtttg accttgtcga gcccatagtg gtcttggtcg aggatcttgc
     1381 gcgcttgatc gatatcgacc gtctcgggat cgaccttcga ccacggcatt gccaacagcc
     1441 agtccaggta ggtccgggtc atcgagtatt cggcggccga gtccggcatg cgctccaagc
     1501 gcttcaattc gcgctgcgcc tgttcttggg cgtccggcgg catgcccgca tccttgatcg
     1561 cttgcttgag ttcttcgagc tcggccggag agttgtcggt ttcgcccagc tctttttgaa
     1621 tctgccgcag gcgttcacgc aatacggctt ccttttgccg ctcgtcgaat gcctcgcgag
     1681 tgcgctcatc gatctgccgc gacactttga ggacctcgat gcgccggccg agcaacgcca
     1741 gcacgcgatc aagccgcgtt ttcaaatcga aggtggccaa gagatcctgc ttatcggccg
     1801 ttttgaggtc catgaagctc gcgacgatgt cggtcagcgc gggaatcgac tcgatgctgc
     1861 ggatcgcgtt cacgagttcg cccggcgcct gcggcagcag ctgcaccgcc tccaacgcct
     1921 tgtcgcgcaa attgagcccg cgcgcttcga cctcgcgatc gaagaccgtc gcctccttgt
     1981 gcgcctcgat ccgcgcgacc agaaagggtt cgcggctgac gaagtcgagc accgtaaatc
     2041 gctcttcgcc ctgcgctatc aggtggtgag tgccgtccgg tgcggtaatg aagcgcacga
     2101 tggtggccgc cgtgccgacg cgatgcagat tatccgggcc cggcgcagcg ccggtcgtat
     2161 ccgtttgcag cacgaggccg acacgccttt gggtacggac cgcctcctgg gcagccgcca
     2221 gcgccgcatc gccgctcacc gcgaccggca gtaccacgcc ggggaacagc accgcgttgg
     2281 cgacgggcaa aattatgagt acatcttccg cgttcatggt gcctgcctcg tgagccgcaa
     2341 atagagacac ccgcggtcaa gccggcgctc gatcagcaca taatgtccgg cgggcaggtc
     2401 gatgcgtcgg cgcattcggc cgtagggaat ttccaggcgc accacgctcg catgcacatc
     2461 gagcgccggc ggcggaacga cggtatcgat ctgcaggccg ttcggggtga tttgtacgac
     2521 gacctcgtcc gcgcgggcgc ccggcagggc gaccacgacc tgaagctcgg atccttgcgt
     2581 gaaaatgtcg gccggcggct cccacgcggg agcggccgcg ggggcggcga gcagttcgaa
     2641 gaagtgccga tgtttgcgct ctgcctgatc gagaagcgca caggcctcgg cccacatcca
     2701 ggactttctg cggtgggtgt tcatgcggtt gaaatgcggc cgcgggcccg gaagttcaag
     2761 ggcccggacg gccctccggc atcagtcgct gctcgcccgc ggcgccggcc ggtcggggat
     2821 tcgcacagta taaccgcccg atttctcatc cgcctgcagt gcgagaagtc cgcgtttctc
     2881 ggcgtccgcc aacagatcgt tgaaggattt gaacccataa gcgcgctcat tgaagccggg
     2941 gtggcggcgc ttgatcgcct gcttgatcat cgagccccag atcggctcgc tgccgccgcg
     3001 ctcctcgccc aaggcatcga gggtgtctac gatcagttcg agggcgtcgt gcaggtcggg
     3061 acccttattc tcgcccggtg cagcttccgc cggggccggc gcgcgcgccg cggggggacg
     3121 gcggcgtgcc ttggagggtt ctttgcgcac cagatcgtcg tagtaaatga attcgtcgca
     3181 attattgatg aaaagatccg aggtcgagtt cttgacgccg acgccgatca cggttttggc
     3241 attttcgcgc aacttgctga ccagcggcga gaagtccgag tcgccgctca gaatgacaaa
     3301 ggtatcgacg tggcccttgg tgtagcacag atcgagcgca tcgacgacca tgcggatgtc
     3361 ggcggagttc ttcccggact ggcgcacatg cggtatttcg atgagttcaa aggccgcctc
     3421 atgcaagccg cgcttgaagt ccttgtagcg atcgaaatcg cagtaggcct ttttgaccac
     3481 gatatggccc ttcagcagca ggcgtgccag caccttctcg atgtcgaaac tcgggtactt
     3541 ggcatcgcgt gcgcccaagg cgatgttctc caggtcgaga aacacggcca tggtggcgtc
     3601 ggatgttctg gttgtcattg gcggggagtt tacgcgagtt agaaccagtt gtaattgaag
     3661 ctgatgctga cccgctccgc agcgatgcga ttcggcacga cttcatgccg cagccaactt
     3721 tcgaacaaca ccaagcggcc ggctcgtgcg ggaaaggtcc cccacggctt ggtctcgcgt
     3781 ttgccgttcg actttcgcgg cggcgccgcc atgaagcgat ccaggcgcgg atcttcgaat
     3841 tttatgccgg gggctcccgc cggcacgcgt acgtagtagg tgccgctcaa ggtcgacagc
     3901 gggtgtaggt gcaggccatg cacgacgtgt tttgccatga tattgatcca gcaatcggtc
     3961 attttgagtt cacgcccatc cagatcccac tccaccgcgc gcgcgaaagc cgcgacatgc
     4021 cgcgcgagtt tgctctccag ggtcttgaag gtcggcgaaa tacgctgcag ccaatgcacc
     4081 gagccgtacg atgtgtagcc ttgcggataa ttcgcggccg accagcggcg tccggccgca
     4141 tcgtcgtggc gcagctgccg gcattctttg agcagttggc ggttgaattc gctgctgccg
     4201 cgcttctgca aggcgtccgc gtacaccaag gttggaaaca gtcggtggat tgtcatcgcg
     4261 caagcgtaac gggcggcgcg cagcgtggca aaataccggc aagatggccc gccgtatcgg
     4321 ataaaatttc ccatgaactt cgcgccgacc gcaccgctgc agcaatcagg caccgcgatt
     4381 ccctggcggg tcgtgccgcc gctcgtcagc acggccctgc tcatgttcgg ctcgggcttc
     4441 ttgggctcgc tgctgccgct gcgtttctcg gctatgggaa tgtccgatgg cgtgatcgga
     4501 ctgatcggca cctgcgaggc cctcggcttt ctcgtcgggt gcctgtacgc gcatcggctg
     4561 atcgcgccgg tgggtctgga tcgggcctat gccacgtttg caagcttcaa gtcggtggca
     4621 atcctcggcc tgtgcttcgc acagagcgtg ccggtcttgt cgctgctgcg gttcatgatc
     4681 ggagtgaacg ccgcggggct cgccgtgatc gtcgagagct ggctgaacgc cttggtgccg
     4741 aacgagcagc gcggccgcgt gctcacgatc tatgtgttgg tatacggtct attcttcggc
     4801 gtgggccagt tgttcagccg cggcttcgac gtcaaagggc cggagatctt gatcatcgcc
     4861 gggatggcga cgacgctcgc actggtaccc atggtggcga tcgacgtgcg ggcgccggcg
     4921 ttgccacacc gcgtcaaact ggagatcctc aaagcgttgc gcacctctcc cgtgagcgtt
     4981 acggcctgtt tgctgaacgg tttgattttg accgccttca ccaccgtcgg acctctgatc
     5041 ggtgtacgga tcggtttcga tcagcagcgg atcgtgctgc tcatggcctg tgtctccttg
     5101 ggcggacttt tcctgcaatg gcccatcggt tacttctcgg acaagatcga tcgtctgcat
     5161 gcgctcatcg gcttagggtt gggcatcctc gcggtggccg ccgcattgat gtgggcagat
     5221 cagcgcatgc cgctgctggt gctggccctg ctgttcggta ttttcggcgg cctcggcgag
     5281 agcctgtacg cggtcggcgt cgcgcacgcc aatgatcgcg cggtggcggc cgactatgtg
     5341 gcgctgtcgt caaccttgct gttcgtgtgg gcattggggt ccgcgctggg tccgaccatt
     5401 ggcacactcg caattcagct ggtcgcgccg ggtgcgttct tcgcctacgt gatcgtattg
     5461 acgctgatat tcaccgtgtt cgcgatctgg aggctatcgc gccgcaaggg cgatcgcatc
     5521 gtcgagtcgc gcgaggagtt tctcgcctac ccacagacct cgccggagat ctacgcctgg
     5581 ctgccctacc ataaagacgc tgcgcacacg gcgccgccga tcaccgccga cgctgcgccg
     5641 gatgcggcaa aaccgccgtg accgacaact caaccaggtg ccccatgaaa acgccggttc
     5701 ttgtgctgct cgcaatcgcc ggatgggcgg cagtgcccgt acgggcggcc gcaggctaca
     5761 cgccgatcac cccgagcatg agcgaccgca cagtgacgct cgatggccac aatctcagca
     5821 tcgatgagat cgtgatggtg gcgcgctatg gagcgaaggt gcagctcaaa cccgaggcgc
     5881 gccagcgcga agcggataat tacggattgc tgttggaagc cgccgccgag ggcataccgg
     5941 tctattggtt caatcgcggc gccggcgatc aacgggaaac cgtcatgttc gatggcgacc
     6001 cgatgtcgcc gaagaaccat gcgctgattg ccaagacgc
//
LOCUS       DASWZT010000403         3046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_51469, whole genome shotgun sequence.
ACCESSION   DASWZT010000403 DASWZT010000000
VERSION     DASWZT010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51469"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..1029
                     /locus_tag="VGE92_11375"
     CDS             139..1029
                     /locus_tag="VGE92_11375"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEY1314472.1"
                     /translation="MTLRALTLAGLVLSGALAQARAPLVLLTDFGTQDGAVSAMKGVA
                     YGVSQDLLISDLSHENPSIFAGAYRLYQTEQFWPAGTVFVAVVDPGVGTKRLSIVLKT
                     RTGHYFVAPDNGLTSLVAERDGIEAVRQIDEHINRRPGSEDSHTFHGRDVFAYTGARL
                     AAGVITFEQVGPELPPQSLIAIAYRKAQRQGEKVSGIVPILDVQFGNVWSNIPKDLFD
                     QLHVPLGTALHVRIYHHGELIDDSVAPYQRTFGDVPVGKPLVYVNSLLNLAVALNQGN
                     YAAAHKIESGPDWSIEIGKN"
     gene            complement(1036..2553)
                     /locus_tag="VGE92_11380"
     CDS             complement(1036..2553)
                     /locus_tag="VGE92_11380"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEY1314473.1"
                     /translation="MRIRHLRIPSRVFLAWLLAGPVYAQSGGLHAPQLPSNHALIITV
                     SEYQRSPLPGVMTDRKLANELAQRFGVPPQNIVELSEQQVTREGLKQAMASMNQVIMP
                     GDKLYVYFSGHGARFFSKVTNQCTESIVMQDMRVVTNTEFAEMLKPLSKKTDKTIVML
                     DSCHSGGIAKVASTRDLGSAGSMRAKYLPEASSPQCAVAVNDRSFSQTRGVDLDTTDN
                     NLVILAASRNNEVAWDTSKGGALTYNFEQCLNGGATDTDHSGSISMQELTACVQARLD
                     KSQEDAARQHATLAGNGALVPAFSAPSQSPSASGGAAPQAAATAAADPLATMNAIYNQ
                     RDDRWQVQARLSQPTLKIGTNLSMSLRSQRDGYVYMFYQGTQPGSFYLLFPNQLDSKN
                     AIRANQDLPLPRPEWNVTALGPHGTDHMLVMISATPRDFSALALPAEYVSAAGAFEKI
                     RPTTPAVAQISQIATLSAAASKPACMTAADKRDSAVASACSNVFGAALVSVDEID"
     gene            complement(2557..>3046)
                     /locus_tag="VGE92_11385"
     CDS             complement(2557..>3046)
                     /locus_tag="VGE92_11385"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HEY1314474.1"
                     /translation="AWLNHVLPANDASLGLRAETDPGSRGDTFVQCESFVFEVKLQKP
                     AYVMVMDLNSDGKLIVLYPTRASEREIIAAGSPRAIPSTDPKARILVTAPFGTDQVAV
                     VAFEKQPSFFADQNGVQPFGSDDARAQAIAKGLAGTSGGVGVQQMAVRTYPATGKVSC
                     GT"
BASE COUNT          543 a          972 c          942 g          589 t
ORIGIN      
        1 gacccacacc ccgctgcgca gttcgccggc accggcggcg gacgtgccgg ccgcggcggc
       61 aacacccgcg acgcccgcaa ccacccccgc gccgactgcc gaagcgccgc gcacctatcc
      121 gctggaacag cagcactagt gactctgcgc gcgttgacac tggcgggtct cgtgctgtcg
      181 ggcgcgctcg cgcaagcgcg cgcgccgctg gtgctgttga ccgatttcgg aacccaggac
      241 ggcgccgtgt ccgccatgaa aggcgtggcg tatggcgtat ctcaggactt gttgatctcc
      301 gacctgagcc acgaaaaccc gagcatcttc gccggtgcct atcggctcta ccagactgag
      361 cagttttggc cggcgggcac ggtgttcgtt gcggtcgtcg accccggagt cggcaccaag
      421 cgcttgtcga tcgtgctcaa aacccgcacc gggcattatt tcgtcgcgcc tgacaacggc
      481 ctcacgtcgc tggtcgccga acgcgatggc atcgaagcag tgcggcaaat cgatgagcat
      541 atcaaccggc gtcccggctc cgaagactcg cataccttcc acgggcgcga tgtgttcgcg
      601 tacaccggcg cgcgcctggc ggcgggggtc atcacgttcg aacaggtggg acccgagcta
      661 ccgccgcaat ccctgatcgc gatcgcgtac cgcaaggcgc aacgccaggg cgagaaggtc
      721 tcgggaatcg ttccgatact cgatgtgcag ttcggcaatg tctggagcaa cataccgaag
      781 gatcttttcg atcaattgca cgtgccgctg ggcacggcat tgcatgtgcg catctatcac
      841 catggtgaac tcatcgacga cagcgtcgca ccctatcagc gcacgttcgg cgacgtgccg
      901 gtgggcaagc cgttggtgta cgtcaacagt ctgttgaatc tcgcggtggc cctcaatcaa
      961 gggaactacg cggccgccca caaaatcgaa tcgggaccgg actggtccat cgaaatcggc
     1021 aagaattgag acttttcagt cgatctcgtc gacgctcacc agcgcggcgc cgaacacatt
     1081 ggaacacgcg ctcgcgacgg cgctatcgcg cttgtcggcg gcagtcatgc aggccggctt
     1141 gctggccgcg gcggatagcg tggcgatttg actgatctgc gccacggccg gcgtcgttgg
     1201 gcgaattttc tcgaacgcac ccgcggcact gacatattcg gcgggcagcg ccagcgccga
     1261 aaaatcccgc ggcgtggcgc tgatcatcac cagcatgtgg tccgtgccgt gcggccccaa
     1321 cgccgttaca ttccactccg gtcgcggcaa cggcaggtcc tggttggcgc ggatcgcgtt
     1381 cttcgagtcg agctgattcg gaaacaacag atagaagctg ccgggctgcg tgccctggta
     1441 gaacatgtac acatacccgt cgcgttgcga gcgcagcgac atcgagaggt tggtgccgat
     1501 tttgagcgtc ggctggctga gcctcgcctg cacctgccag cggtcatccc gctgattgta
     1561 gatggcgttc atcgtcgcga gcggatccgc tgcggcagtc gccgcagcct gcggtgccgc
     1621 gccgccgctc gcgctggggc tctgacttgg cgcagaaaac gccggcacca gcgcgccgtt
     1681 gcctgcaagc gtcgcatgct ggcgtgccgc gtcctcctgc gacttgtcga gccgcgcctg
     1741 aacgcaagcg gtgagttctt gcatcgaaat cgagccgctg tgatcggtat cggtcgcgcc
     1801 gccgttcaag cactgctcga agttgtaggt gagcgcaccg cccttggagg tgtcccaggc
     1861 aacctcgttg ttcctcgagg cggcgagaat caccagattg ttatccgtcg tatcgaggtc
     1921 gacgccgcga gtctgcgaga agctgcggtc attcaccgcc accgcgcatt gcggactcga
     1981 agcctccggc aggtactttg cacgcatcga gcccgccgac cccaagtcgc gtgtgctggc
     2041 caccttggcg atgccgccag aatgacaaga atcgagcatg acgatggtct tgtcggtttt
     2101 cttactgagc ggtttgagca tctcggcgaa ttcggtattg gtcaccaccc gcatatcctg
     2161 catcacgatg ctttccgtgc actggttcgt caccttgctg aagaagcgcg cgccgtgccc
     2221 ggagaagtac acgtacagtt tatcgcccgg catgatcacc tggttcatgc tcgccatcgc
     2281 ctgtttcaag ccctcgcgag tcacttgctg ctcagagagc tcgacgatgt tttgcggcgg
     2341 caccccgaaa cgctgcgcca attcgttggc aagtttcctg tcggtcatca cgcccggcaa
     2401 gggactgcgc tggtattcgg ataccgtgat gatcagcgcg tggttcgacg gcagttgcgg
     2461 cgcgtgtaat ccacccgatt gggcatacac gggaccggca agcagccacg ccagaaaaac
     2521 tcgggacggt attcgcagat gtcgaattcg catgatttaa gttccgcagg agactttgcc
     2581 ggtggcgggg taggttcgca ccgccatttg ctgcacgccg acgccgcctg aggtgcctgc
     2641 caaacccttg gcgatcgcct gcgcgcgggc gtcatccgag ccaaagggct gcacgccgtt
     2701 ctggtcggcg aagaagcttg gctgcttttc gaacgccact acggcaactt ggtccgtgcc
     2761 aaacggggcc gtcaccagaa tccgcgcctt ggggtcagtg cttggaattg cgcgcggcga
     2821 ccccgcggcg atgatttccc gttccgacgc ccgcgtcgga tagagcacaa tcagtttgcc
     2881 gtcgctgttg aggtccatga ccatcacata cgccggcttt tgcagcttaa cttcgaacac
     2941 gaaagattcg cattgtacga aggtgtcgcc gcgacttccc gggtcagtct cggcgcgcag
     3001 gccgaggctc gcgtcgttcg cgggcaagac atggttcaac catgcc
//
LOCUS       DASWZT010000404         7987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_7965, whole genome shotgun sequence.
ACCESSION   DASWZT010000404 DASWZT010000000
VERSION     DASWZT010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7987
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7965"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VGE92_11390"
     CDS             complement(<1..368)
                     /locus_tag="VGE92_11390"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEY1314475.1"
                     /translation="MTFSLVARSADAHLFGVAIVSSSPAVAARCAHARAGVGAVATQN
                     ITDPSLGGRALDMLGRGASAQAALDQVLSTTPFGAYRQLMTIGRSGAPAIFSGAHGLG
                     LVSSASGADAAAAGNLLASSA"
     gene            complement(372..785)
                     /locus_tag="VGE92_11395"
     CDS             complement(372..785)
                     /locus_tag="VGE92_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004139129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEY1314476.1"
                     /translation="MHTRIRKFNTRDTYPEQKLDNDLCQTVVAKGRSVFVRGQVGQDL
                     DTAVNVGIGDAAAQAEQAMTNIKQLLSEAGATLEHICKVTIYLTDPRFREAVYRVIGR
                     CLRGVYPVSTGIVVAGLARPEWLVEIDVIAVIPDD"
     gene            complement(787..2235)
                     /locus_tag="VGE92_11400"
     CDS             complement(787..2235)
                     /locus_tag="VGE92_11400"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase family
                     protein"
                     /protein_id="HEY1314477.1"
                     /translation="MIRTGRQYLDSIRDGREVYINGTRVADVARHPMFKPLVDIRARI
                     YDMQHEGGTRNTLAYLEGDEWHAVANKLPRSAADWQDKRLATDAVLDEIGGVVTRVGD
                     ETVGEMWSLFDGQEVLNEVDPQFSANIRTHIDAVLHADPFHVSANTDPKGDRSKPPQE
                     QDPDMLLHVVKETDAGIVVRGAKYETAAPYANQAFVKPTNANWGSAALSDYAVGFICD
                     LSSQNLKFVCRTGFAGRAAAADYPLANKFDEIDALVIFDDVLIPWENVLFYRHTEAAA
                     FIRATLHRYSAFAFVQRILKFADMMIGAALYNVRQTGLDKQQAVQEKLAELAVYREGI
                     HAHLTAAVALAEPSPAGLLMPNQSLLYTGRVLACSQLHHMMHVARELCGGQICVTPDK
                     ASFDAPETKPWLDKFYSVNDNWIAEDRRKLLAFARDLLNSDYAGHRLTFQLFAQSPPF
                     AHLGAVYRNFDWDGPLDFVRKAADLSTRVTRQ"
     gene            complement(2289..4736)
                     /locus_tag="VGE92_11405"
     CDS             complement(2289..4736)
                     /locus_tag="VGE92_11405"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1314478.1"
                     /translation="MKTNPRARQPLVVGVTSVTAVLTALHGEAALADDDALQEVVVTA
                     SRRAESAQDLPISITAVTGASLDQAGIADIAGLAHSMAGVNFTDKGPFGGVNGSTLII
                     RGLNSEATGGQIALSTPIVPPVATYVDDTALFVNLRLEDLDHVEILRGPQGTLYGSGS
                     LGGTIRFVQNAPDPSGFDARAEAGVSKTNHTHALNEDVSGMLNLPLSDTFAVRLNASW
                     TDEAGFINQPNLYVLDSSGAPIAAQPGNVFSPPQTFSQDGTNAYGYRTARVAALWRPS
                     DTFKAQLSYHYQISTADGFPWSSPQLGVQSLSSTDHTQAASYDKVDLFALTLEGDLGF
                     ATLTSNSSWADHNNHSSTDLTNLYDHFSFYSQLYGANPRVLVTGHDELIDKPWSQEIR
                     LASKAGGTFDWVGGLFFKNQTTLIQEHEYYNGYQAFYNACEPIYGQNVDFNQTSECGT
                     GYTPGTPTVIDGIPILKDQAYIGDFETHFKDLAVFGEVTWHLTSDWDLTGGSRVFKQT
                     LTQAQQTGLLFDGDPALFGPVLPIANLSLSDTWRKALWKVNTSYKLDKTNLVYATWSQ
                     GFRRGSVNALPLAEPAEGYTTPSALLKVSPDTADNYEIGAKGTLANRVRYSAAIYDIQ
                     WHNVQEGAQLTPLVLPGAINLGEAYSRGVEAEISANITDHISGQLDYTYDETKVTSYA
                     ALAQGAGNLSVDLPALGGPLPGTPKNSLAVGLVYGHIPFAGGEMRYAVNARYQSKLLP
                     AISASIPTVSGYTMVDARASYEVAHWLGTLYVENLTNQLGIDSYSDPANYGTNYQALV
                     SRPRTVGISIAYSFKEH"
     regulatory      complement(4854..4957)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
     gene            complement(5107..6702)
                     /locus_tag="VGE92_11410"
     CDS             complement(5107..6702)
                     /locus_tag="VGE92_11410"
                     /inference="COORDINATES: protein motif:HMM:NF020354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEY1314479.1"
                     /translation="MRAIAGNRAALDQFYYLKNFETVLDSLRERYADLLSAEEHGFME
                     RFRAMPQASRALLVRMVMRKGELFRASALRYAEIGATHAVAAPLAQAGWVDVEPHLTL
                     EELQALLTKAELVRHFALPGRELQRKKSDLAALLRQQYSQAKPFLDWCPGSGERVYRL
                     TVAALCERFRLMFFGNFRQDWSAFVLADLGIFSFEKIDLAVQSRAFRSREDVDVLLQL
                     QRCREYLNSTTPLDTVVRSVPEEVRDCDWLEERRQKLLFDVARALERSGDTLAACAIY
                     SRCTHSGAHERGVRLYRREQRKLGIRCAATPRATRNPPELPSFELVLDQPVPGCAIEY
                     LARNHLAQDMPHGATVHYVENSLINSLFGLLCWRAIFAPIAGAFFHDFHRGPADLSSA
                     DFYRRREREFAGCFAELESGRYQMTIRETYTRKVGIQSPFVAWNLLSESLLAHALACL
                     PPEHLRHWFEWIARDVPRNRAGFPDLIQLWPRERRYRMIEVKAPGDRLQDNQRRLLQF
                     CAGHGMPVAVCYVRWARPNVAQS"
     gene            complement(6719..>7987)
                     /locus_tag="VGE92_11415"
     CDS             complement(6719..>7987)
                     /locus_tag="VGE92_11415"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF002550.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEY1314480.1"
                     /translation="RQEGGNEYRGASGKLKTRYGSLRNPLHTAWLAAAREAGYPRTDD
                     VNGFQQEGFGRMDMTVGEGRRCSAANAYLRPAMRRANLKVLTHALASRIVFEGRRAVG
                     LEYTRGGDTHRVDAREVILSAGPINSPQLLKLSGVGPAAELRTLGISVVHDLPGVGEN
                     LQDHLEFYFQVACREPVSLYSSINLWNRALIGARWMLRKDGLGATNHFESCGFIRSRA
                     GVPYPDIQYHFLPMAVAYDGSTLAQEHGFQAHVGPMRSKSRGWVRLASTNPRDKPRIL
                     FNYLNHPDDWTEMRACVRLTREIFAQPAFDRYRGREIQPGTLVQTDEQIDAFIRAKVE
                     SAYHPSCSCKMGSAQDSMAVVAPDTRVHGLDGLRVVDSSIMPSVTTGNLNAPTIMLAE
                     KAADHILGSGMLAPEDAPYYVAPNWRAAQR"
BASE COUNT         1488 a         2493 c         2494 g         1512 t
ORIGIN      
        1 gcgctgctcg ccagcaaatt tccggcggcc gccgcatcgg ctccgctcgc cgagctcacg
       61 agacccaatc catgcgcgcc cgaaaagatc gccggcgcac cgctgcggcc gatggtcatg
      121 agttgccgat aggccccgaa gggggtcgtc gacaacacct gatcgagagc cgcctgggcg
      181 ctggctccgc gtccgagcat atccagagcg cggcctccga ggctcgggtc ggtgatgttt
      241 tgcgtcgcca ccgctccgac cccggcgcgt gcgtgggcgc agcgcgccgc gacggccggc
      301 gaggacgaaa cgatcgccac gccgaatagg tgcgcatcgg cggagcgcgc aaccagtgaa
      361 aatgtcatgc ctcagtcatc cggaatcacc gcgatcacgt cgatctccac cagccattcc
      421 gggcgtgcca agcctgcaac cacgatcccc gtcgacaccg gatacacgcc gcgcaagcag
      481 cgaccgatca cccgatacac ggcttcgcga aaccgcgggt cggtcaggta gatggtcacc
      541 ttgcagatgt gttcgagcgt agcgccggct tcgctcaaca gttgcttgat attcgtcatc
      601 gcctgttccg cttgcgcggc cgcatcgccg atgccgacgt tgaccgccgt atcgagatcc
      661 tggccgactt gaccgcgcac gaacaccgag cggcccttgg cgaccaccgt ctggcacagg
      721 tcgttgtcga gcttctgctc cgggtaggtg tcgcgcgtat tgaacttgcg aatccgcgtg
      781 tgcatgttac tgcctcgtga cgcgagtgga aagatccgcg gccttgcgaa cgaaatcgag
      841 cgggccatcc cagtcgaagt ttcgatacac ggcgcccaag tgcgcgaacg gcggcgactg
      901 cgcgaacaat tggaaggtca gccgatggcc cgcgtagtcc gagttcagca ggtcgcgcgc
      961 aaatgcgagc agcttgcgcc gatcctccgc gatccagttg tcgttgaccg agtagaattt
     1021 gtcgagccac ggcttggtct ccggtgcatc gaacgacgcc ttatccggag tgacgcaaat
     1081 ctggccgccg cacagctcac gtgccacgtg catcatgtga tgcagctgcg agcacgcgag
     1141 gacgcggcct gtatacagga gcgactggtt cggcatcagc aggcccgccg ggctcggctc
     1201 cgcgagtgcg accgcagccg tcagatgcgc gtgtattccc tcgcggtaca ccgcaagttc
     1261 ggcaagtttt tcctggaccg cctgttgctt gtcgagtccg gtttggcgca cgttgtacag
     1321 cgccgcgccg atcatcatat cggcgaattt taaaatacgc tgcacgaacg caaacgccga
     1381 gtagcgatgc aacgtcgcgc ggataaatgc ggcggcctcg gtgtgccggt aaaacagcac
     1441 attttcccaa gggatcagta cgtcatcgaa aatgaccagc gcgtcgatct cgtcgaactt
     1501 gttggcgagc ggatagtccg ccgcggcggc gcggccggcg aaacccgtgc ggcacacgaa
     1561 cttgagattt tgcgagctca agtcgcagat gaagccgacg gcatagtccg agagcgcggc
     1621 gctgccccaa ttggcgttgg tcggcttgac gaaagcctga ttggcatacg gcgcggccgt
     1681 ttcgtacttg gcgccgcgca cgacgatgcc ggcgtcggtt tctttgacga cgtgcagcag
     1741 catgtcgggg tcctgctcct ggggaggctt ggagcggtcc cccttggggt cggtgttggc
     1801 agagacgtga aacggatccg cgtgcagcac cgcatcgata tgcgtgcgaa tgttggccga
     1861 gaactgtgga tcgacctcgt tgagcacctc ttgaccgtcg aacaacgacc acatctcgcc
     1921 cacggtttcg tcgccgacgc gggtcacgac gccgccgatc tcatcgagga ccgcatcggt
     1981 cgccaggcgc ttgtcctgcc aatcggctgc gctgcgcggc agcttgttcg cgaccgcgtg
     2041 ccactcgtcc ccctcgagat aggccagcgt gttgcgggtg ccgccctcgt gctgcatgtc
     2101 gtaaatccgg gcgcgaatgt cgaccagggg tttgaacatg ggatgccgcg cgacatccgc
     2161 gacccgcgtg ccgttgatgt atacctcgcg cccgtcgcgg atcgaatcga ggtactgccg
     2221 gccggtacgg atcatggctc actccacgag aacttggggc ccatcatggc gcaggtccgc
     2281 gcccgctcct agtgttcctt gaacgaatag gcgatcgaaa tccccactgt gcgcgggcgc
     2341 gataccaagg cctgatagtt cgtgccgtag ttggcggggt ccgagtagga atcgattccc
     2401 agctggttgg tcaggttctc gacgtaaagc gtgccgagcc aatgcgccac ttcgtagctg
     2461 gcacgggcat cgaccatggt gtagccggag acggtgggta ttgacgccga gatcgccggc
     2521 agcagcttgc tttgatagcg cgcgttgacc gcatagcgca tctcgccgcc ggcaaacgga
     2581 atgtggccgt agaccagccc gacggctaag ctatttttcg gcgtgcccgg cagcggtccg
     2641 cccaaggcag gcagatcgac ggacaaattt ccggcaccct gcgcgagcgc cgcgtagctc
     2701 gttaccttgg tctcatcgta cgtgtagtcc aactgcccgc tgatgtggtc ggtgatgttc
     2761 gccgagatct cggcctccac gccgcggcta taggcctcgc ccaagttgat ggctccgggc
     2821 aataccagcg gtgtcaattg cgcgccttcc tgtacgttat gccactggat atcgtagatt
     2881 gcggcggagt agcgcacacg atttgcaagc gttcccttgg cgccgatttc gtagttgtcg
     2941 gccgtatccg gcgacacttt gagcagggcg ctcggcgtcg tgtagccttc cgcgggctcg
     3001 gccagcggca acgcgttgac gctgccgcgg cgaaaaccct gcgaccaggt cgcgtacacg
     3061 agattggttt tatcgagttt gtacgaggta ttgaccttcc acagtgcttt gcgccacgtg
     3121 tcgctcaagc tcaaattggc gatcggcagc accggtccaa acagagccgg atcgccgtcg
     3181 aacagcaacc cggtctgctg cgcttgcgtc aaggtctgct tgaaaacgcg cgagccgccg
     3241 gtgaggtccc agtcggacgt cagatgccag gtaacctcgc cgaatacggc gaggtccttg
     3301 aagtgagttt caaagtcgcc gatgtacgcc tgatccttca atatgggaat gccgtcgatg
     3361 acggtggggg tccccggcgt atagccggtg ccgcactcgg aggtctggtt gaaatcgaca
     3421 ttctggccgt agatcggctc gcaggcgttg taaaacgcct gataaccgtt gtaatactca
     3481 tgctcctgga tcagcgtcgt ctgattcttg aagaacaacc cgccgaccca atcgaaagtc
     3541 ccgcccgcct tcgaggccaa gcgaatttcc tgcgaccagg gtttgtcgat caactcgtca
     3601 tgaccggtca ccagcacgcg gggattcgcg ccgtagagtt gcgaatagaa gctgaagtga
     3661 tcgtacaggt tggtaaggtc ggtgctggag tggttgttgt ggtctgccca ggaggaattg
     3721 gacgtcagag tggcaaagcc gagatcgccc tccagcgtca gtgcaaacag atccaccttg
     3781 tcgtagctcg cggcctgggt gtggtcggtg gatgacagcg actgcacgcc cagctgcggc
     3841 gaggaccacg ggaatccgtc cgcggtggat atttggtagt gatacgagag ttgcgccttg
     3901 aatgtgtcgc tcggcctcca cagcgcggca acgcgcgctg tgcggtaccc gtacgcattg
     3961 gtgccatcct gcgaaaaggt ctgcggcggg ctgaacacgt tgccgggttg cgcggcaatc
     4021 ggcgcgccgc tcgaatcgag tacgtagaga ttcggctgat tgatgaaacc tgcttcatcc
     4081 gtccaactgg cattcaggcg cacggcgaac gtgtcggaca gcggtaaatt caacatcccg
     4141 ctcacatctt cattgagcgc gtgtgtgtga ttcgtcttgc tcactccagc ctcggctcga
     4201 gcatcgaagc cggagggatc gggcgcattc tgtacgaagc ggatggtgcc gccgagcgac
     4261 cctgagccat acagtgttcc ttgcggtccg cgcaggatct ccacgtggtc gagatcttcc
     4321 agacgcagat tgacgaacag agcggtatcg tcgacgtagg tcgcgaccgg gggaacgatg
     4381 ggcgtcgaca gcgcgatctg tccgccggtc gcttcgctat tgaggccgcg aatgatgagg
     4441 gtcgagccat tgacgccgcc gaaaggacct ttgtcggtga aattcacgcc cgccatcgaa
     4501 tgcgcgagac ccgcgatatc cgcgatgccc gcctgatcga gcgatgcgcc cgtgacggct
     4561 gtaatgctga tcggcaaatc ttgcgccgac tcggcgcggc ggctcgccgt gacgacgacc
     4621 tcttgcaagg catcgtcgtc ggccagcgca gcttccccat ggagcgccgt gagcaccgcc
     4681 gtgactgatg tgacaccgac tacgagcggc tgtcgagcgc ggggatttgt cttcattcga
     4741 gaactcctcg tcaagcgtgg acatgccacc catgaggagc tctgcgccgg cttcgggatt
     4801 ttccctggta cgtagggaga ccttcccgag gacgcgaacg ctcctcgact ttgatctccc
     4861 gggcttttat cccgccgtgt atccttaaac tctaaggact gcctctctcg gaccagtcgc
     4921 ctgccgcagc aaagccggaa ccctagacgc acattgtatt tagcattgtg caaaacccgt
     4981 gccgggtgca tgcggacggc accgcgcaga cgaatttgcc gtaaaaggcg cagtttttat
     5041 ttgctcgcgc agcgccgctc gcttgcgaca ccgcaccgca tgcgggcact tgcgcgagtc
     5101 ggcgccctaa ctttgcgcga cgtttggccg cgcccagcgc acgtagcaca cggcgacagg
     5161 catgccgtgt cccgcgcaga attgcagcag tcgccgctgg ttgtcctgca gccggtcgcc
     5221 gggtgccttc acttcgatca tgcgataacg ccgctcgcgc ggccacaatt ggatcaaatc
     5281 cgggaatccc gcgcggttgc gcggcacatc gcgcgcgatc cattcgaacc agtgccgcaa
     5341 atgctcgggc ggcagacacg caagcgcatg cgcgagcagc gattcgctca gaagattcca
     5401 tgcaacgaac ggcgactgga tgccgacctt tcgcgtatag gtctcgcgta tcgtcatttg
     5461 ataacgccca gattccaact ccgcgaagca ccccgcaaat tctcgctcgc ggcggcgata
     5521 gaaatcggcg ctggataagt cggccggccc gcggtgaaaa tcatggaaga aggccccggc
     5581 aatcggcgca aatatcgcgc gccagcacag caatccgaac agcgaattga tgagcgagtt
     5641 ttcgacgtaa tgcaccgttg cgccgtgcgg catgtcttgc gccaggtgat ttcgcgcaag
     5701 gtattcaatc gcgcatccgg gcacgggctg gtcgagcacc aattcgaagc tgggcagttc
     5761 cggcggattg cgcgttgcgc gcggcgtcgc ggcgcagcgg atgcccaact tgcgctgttc
     5821 gcgccgatac agccgcacgc cgcgctcgtg cgcgcccgaa tgagtgcatc gtgaatagat
     5881 cgcgcatgcc gcgagcgtat cgccgctgcg ctcgagcgca cgcgcaacgt cgaacagaag
     5941 cttctgccgc cgctcctcca gccaatcgca gtcgcgcacc tcctcaggca cgctgcgcac
     6001 caccgtgtcc aacggcgtgg ttgaattcag gtactcccgg cagcgctgca gttgcagcaa
     6061 cacatcgacg tcctcgcggc tgcgaaaagc acgcgattgc acggccaggt cgattttctc
     6121 gaagctgaaa atgccaagat cggccaatac gaatgcagac caatcctggc ggaaattgcc
     6181 gaagaacatg agtcggaacc gctcgcagag cgctgcgaca gtgagtcgat acacccgctc
     6241 gcccgagccg gggcaccagt ctagaaacgg cttcgcttgg ctatattgtt gacgaagcag
     6301 cgccgcaaga tcggatttct tgcgttgcag ctcgcgcccg ggcagagcaa aatgcctcac
     6361 caattcagcc ttggtgagca gcgcctgcag ctcctcgaga gtgaggtgcg gttcgacatc
     6421 gacccagccg gcttgcgcga gcggcgcggc cacggcgtgc gtcgcgccga tctccgcata
     6481 gcgcaacgca ctcgcccgaa acagctctcc cttgcgcatg accatgcgca ccaggagcgc
     6541 gcgcgaggct tgaggcatcg cgcgaaagcg ctccatgaag ccgtgttcct ccgcggacag
     6601 cagatcggcg tagcgctcgc gcagcgaatc gagcaccgtc tcgaagttct tcaggtaata
     6661 gaattgatcg agcgcagcgc gattgccggc gatggcccgc atgcgctcga ttcttcggct
     6721 agcgctgcgc cgcgcgccag tttggcgcca catagtacgg cgcatcctcg ggcgccagca
     6781 tgccgcttcc caagatgtga tcggcggctt tctcggccag catgatggtt ggtgcgttca
     6841 gattaccggt ggtcaccgac ggcatgatgg aagaatccac gacgcgcagc ccgtcgagtc
     6901 catggacccg cgtatccggg gcgaccaccg ccatggaatc ttgcgcgctc cccatcttgc
     6961 aggaacatga ggggtggtag gcgctctcga ccttggcgcg gatgaaggca tcgatctgct
     7021 cgtcggtctg aactagggtg ccgggttgaa tctctcggcc ccgatatcga tcgaatgccg
     7081 gctgcgcgaa tatttcccgc gtcaggcgca cgcaggcgcg catttcggtc caatcgtcag
     7141 gatggttcaa ataattgaac agaatgcgcg gtttgtcgcg cgggttggtc gaggccaagc
     7201 gcacccagcc gcggctcttg ctgcgcatcg gcccgacgtg cgcctggaag ccgtgttcct
     7261 gtgccaaggt cgagccatcg taggccaccg ccatcggcag aaagtgatac tggatgtccg
     7321 gatacggcac acccgcgcgg ctgcggatga agccgcagga ctcgaaatga ttggtcgcgc
     7381 ccaatccgtc cttgcgcagc atccagcgcg cgccgatcaa tgcgcgattc cacaggttga
     7441 tcgacgaata cagggacacc ggctcccggc aggccacctg aaaataaaat tccaaatgat
     7501 cctgcaagtt ctcgccgacc ccgggcaagt cgtgcaccac gctgatgccg agggtgcgca
     7561 attctgccgc ggggccgacg cccgagagct tgagcagttg cggtgaattg atcggacccg
     7621 cggataggat cacctcgcgc gcgtcgacgc gatgcgtgtc gccgcctcgg gtgtattcga
     7681 ggccgacggc gcgccggcct tcgaacacga tgcggctcgc cagggcgtgg gtcaggactt
     7741 tgaggttggc gcggcgcatg gccggccgca ggtacgcgtt cgccgcgctg cagcgccggc
     7801 cctcgcccac cgtcatgtcc atgcgaccga atccttcttg ctgaaaaccg ttcacgtcat
     7861 cggtgcgcgg atatcccgct tcgcgggcgg cggcaagcca cgccgtgtgc aacggattac
     7921 gcaggctgcc atagcgggtt ttcagcttgc ctgaagcgcc gcgatattca ttgcctcctt
     7981 cttggcg
//
LOCUS       DASWZT010000405         8736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_22731, whole genome shotgun sequence.
ACCESSION   DASWZT010000405 DASWZT010000000
VERSION     DASWZT010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8736
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22731"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(307..906)
                     /locus_tag="VGE92_11420"
     CDS             complement(307..906)
                     /locus_tag="VGE92_11420"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEY1314481.1"
                     /translation="MDADSTSAAAALTAAGIWIQDGFLALSVRDALKQCAQARRQRGD
                     FAAARIGGAQSVTRRADIRGDSTCWISPPLLPAEAALLGELERLRLELNRDCLLGLFD
                     LELHYAWYAPGAGYARHVDQPRGSTQRQVSLVLYLNDQWTPAAGGELRIFDAHGGHRD
                     IAPLAGRLVCFLTPNREHAVRETQQDRLSISGWFRAREG"
     gene            complement(896..1429)
                     /locus_tag="VGE92_11425"
     CDS             complement(896..1429)
                     /locus_tag="VGE92_11425"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEY1314482.1"
                     /translation="MKKPPKRRRTPLFEVRHSPIHGYGVFAARRIRKGTTVIEYLGER
                     VSHAQADARYEDKDPNDNHTFLFTVDAKTVIDAGVNGNEARYINHGCAPNCESTTLRK
                     RIFIEAIRTIQPGEELSYDYQIQRDLDDAPDVDEIYACRCGAQNCRGSMLEAPKKGGS
                     AKRAGRRDGVRSQRRGR"
     gene            complement(1426..2220)
                     /gene="thyA"
                     /locus_tag="VGE92_11430"
     CDS             complement(1426..2220)
                     /gene="thyA"
                     /locus_tag="VGE92_11430"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEY1314483.1"
                     /translation="MRQYLDFLRNIRDYGARKEDRTGTGTLSLFGYQMRFDLADGFPL
                     VTTKKLHLPSIIYELLWFLRGDTNVKYLHEHGVRIWDEWADEDGNLGPVYGKQWRSWP
                     SADGGNIDQLARVVEQLKSDPNSRRILVSAWNVGELQQMALLPCHALFQFYVANGRLS
                     CQLYQRSADALLGVPFNIASYALLTHMLAQQCDLRVGEFIWTGGDCHLYMNHLEQAEL
                     QLARAPYPLPRLVIRRKPASLFDYEFEDFAILDYRSHAAIKAPISV"
     gene            complement(2220..3056)
                     /gene="lgt"
                     /locus_tag="VGE92_11435"
     CDS             complement(2220..3056)
                     /gene="lgt"
                     /locus_tag="VGE92_11435"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY1314484.1"
                     /translation="MLTYPHINPVAISIGPVFGIGPLRVHWYGIMYLIGFVAAWWLAR
                     YRARRPGSTWSALDIDDLIFYCAIGVIVGGRVGWCIFYGHDVIAESWLNVFHIWDGGM
                     SFHGGMSGVLVAAIVFGRVKGKRLDDVLDFVAPLPGIGLLAGRLGNFINGELWGKPTD
                     LPWGFGVPNAGGELIARHPSQLYEAGLEGLVLFIIVWWFTSKPRPRLAPTGLFLLIYG
                     CARFSVEWVRLPDANIGYLAGSWLTMGMLLTMPMIVAGAAMMIYAYRRDAASGNLGAV
                     SA"
     gene            complement(3061..4272)
                     /locus_tag="VGE92_11440"
     CDS             complement(3061..4272)
                     /locus_tag="VGE92_11440"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019097921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEY1314485.1"
                     /translation="MSDSARAAELPALRLKRNEDRRLHAGHLWIFSNEIDTQQTPLGK
                     FKPGELVRVLAHNDRGLGLAYVNPQSLISARLLETWQPPDAAWLATRMRAALALRERL
                     YAAPYYRLVYGESDGLPGLVIDRYGSACVVQIGTAGMERLKLPIQHALEQVLQCEAVL
                     FKNDSAAREMEGLPSYIEAATGRFDELHTVVEDGLEFQAALAAGQKTGWFFDQAGNRR
                     AMVNYVRKGARVLDVFSYAGAWGVRAAVSGARDVLCVDSSAAALELAAANAERNGVKL
                     DTLHGDAFDVLEDLAKKRARYDLVIIDPPAFAKRKKDLPKALAAYKRLNQLAMQVLVD
                     DGILVSCACSYHVSPEELQDAIGKAARSAQKHLQILQTGGQAADHPVHPAIPETRYLK
                     AYFCRVNESLK"
     gene            4326..6362
                     /locus_tag="VGE92_11445"
     CDS             4326..6362
                     /locus_tag="VGE92_11445"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY1314486.1"
                     /translation="MQRNRLAAQTSPYLKQHADNPVDWYPWGAEALQAAERADKPILL
                     SIGYSACHWCHVMAHESFEDPATAALMNRLFVNIKVDREERPDLDKIYQVAQALLTHG
                     SGGWPLTMFLTPRDQSPFFGGTYFPKQPRHGMPAFGDLLMRVAEYYRTHSAEIAQQNA
                     QLRAAYAALTPPAAARDVALDRGPLRAARAALERTFDARFGGFTQAPKFPHPGSIERC
                     LRHWHDTRGAAAPDLQALFMASLTLTRMAEGGIYDQLGGGFARYSVDAEWSIPHFEKM
                     LDDNAQLLCEYARAALATGDAHFARIAAETAQWALRDLHSLEGGFYSSLDADSEGHEG
                     KFYVWTPAQIQALLTSQEYAAFARRFGLERGANFEGQWHLRVTESNDDIAVAAHIDSA
                     RAKLLQARNLRVSPARDEKILTAWNALMIKGLAIAARVLRRADLAHAATAAVDFVRRR
                     LWRDGRLLATYKDGRAHLPAYLDDYAFLADALLELLQTRWRSSDLEFAQALAQVLLSQ
                     FEDDADGGFFFTAKDHERLIHRSKTFSDESLPSGNGVAAAVLSRLGYLLGESSYLEAA
                     ERTLRAGWPMLEQYPQAHMSLLNALDDFLSPVQVLIIRGDAPQAAHWSAQLGALYAPT
                     RMIFAIPRDAEALPPALGVKAAAAGTVAYLCRGTTCSAPFTDLEAIARELALRV"
     gene            complement(6359..6817)
                     /locus_tag="VGE92_11450"
     CDS             complement(6359..6817)
                     /locus_tag="VGE92_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314487.1"
                     /translation="MSAPTLIHEIVDRCRAGTYPAAVARLGSGWVVMGERQVLTGYCL
                     LLADPVVPHLNTLAPDLRGQFLSDMAVVGDALLAVTAGLRINYALFGNVDPALHAHIF
                     PRHASEPEATRTLQPWALDWAAAPRYCEPLHGELQRRLAARLSECSEPAR"
     gene            complement(6814..7218)
                     /locus_tag="VGE92_11455"
     CDS             complement(6814..7218)
                     /locus_tag="VGE92_11455"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY1314488.1"
                     /translation="MSIILLSAALAACAPKTPGAAQSGGAPAAADAGQRIYNGNCLPC
                     HQETGAGIAGVYPSLAGSPVVLGDPAELARWVLKGQRPKSMPTGRYSTQMLQFGWLKP
                     KDAAALLSYLRSSFGNSAPPVDADAVAKALGP"
     gene            7277..>8736
                     /locus_tag="VGE92_11460"
     CDS             7277..>8736
                     /locus_tag="VGE92_11460"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY1314489.1"
                     /translation="MMRLATYWLAAAAALFAAAALAQAPAEDALKRLIEEGNPAYLSD
                     LASANDRAEIGALYAANAYRLLWSDGAKPTAQAIALLQQLRLAGERGLDPEDYPGNRL
                     AYLLVDLIDAPHSGIEQWALFDAGLSLAGMRFLSDVHIGRVDPATMGRDLTVGRVKLD
                     IPTTLGHLASAVDVPLAIDALEPQFSHYALLKKQLSHYRALALEDGLTALPALPAKSL
                     KMGDTYSGAAQLQRLLVALGDEAAPAAAAPAPQTLSPPLVDALKHFQSRHGEKPDGVL
                     GAATFAELTRPLGERVKQIELTMERWRWLPSELETAPIIVNIPQFRLFAFESTADSEA
                     HIRQMDVIDGKAFDKTQTPVFAADMTYLIFRPYWEVPYDIALKEVVPTARNNPADIER
                     RQMEIVRGNGDSAVVMPNTPENVELVARGTLRLRQRPGPNNSLGLVKFLFPNPYNVYL
                     HSTPAQSLFAESRRDFSHGCVRVSDPVGLAEYVLRDS"
BASE COUNT         1602 a         2876 c         2743 g         1515 t
ORIGIN      
        1 acgggccgcc gccgacggcg atgggatttc ccggcggact cgtgcccgtg ttgccggtgt
       61 ttgcgtcgct gacttcggtg ccgcgactgc tgttatagga actggatccg cagccggata
      121 acagcaacag cagtgcaccg agcaggagtg ggtgacggat gagcagcatc tttcgcgggc
      181 accggcggac gggcattcaa agtaatgccg ggatggaacc ctcggtgtta actgcgtcac
      241 gctctgcaag gggagcgcgc gctcgacata actcgacgcc gtacacaagc ggcgacagaa
      301 atatgttcaa ccttcgcgcg cgcgaaacca accgctgatg ctcaatcgat cctgctgcgt
      361 ttcgcgcacg gcatgctcgc ggttcggcgt gaggaaacac accagtcgcc cggcgagcgg
      421 cgcgatatcc cgatgaccgc cgtgcgcatc gaaaatacgc aactcgcctc ccgccgcggg
      481 agtccactga tcgttcaagt acaaaaccag cgacacctga cgctgcgtgc tgccgcgcgg
      541 ctgatcgaca tggcgagcgt aacctgcgcc cggcgcatac caggcgtagt gcagctcgag
      601 atcgaacagt ccgagcaggc aatcgcgatt cagctcgagg cgcaatcgct cgagttcgcc
      661 gagcaaggcc gcttccgcgg gcaataacgg cgggctaatc cagcaggtcg agtcgccgcg
      721 aatatccgcc cgacgcgtaa cgctttgcgc gccgccgata cgcgccgccg cgaagtcgcc
      781 gcgttgccgg cgggcttgcg cgcattgctt gagcgcgtcc cgcacagaga gcgcgagaaa
      841 gccgtcctga atccagatgc cggccgccgt cagggccgcc gccgccgaag tgctatcagc
      901 gtccacgccg ctgcgagcgg acgccgtcac gccgccccgc gcgcttggcc gagccgcctt
      961 tcttgggcgc ctcgagcatg ctgccgcggc aattctgggc tccgcagcgg catgcataga
     1021 tctcatcgac atcgggggcg tcgtcgaggt cgcgctgaat ttgatagtcg taggataatt
     1081 cttcgcccgg ctggatggtg cgaatggcct cgatgaagat acgcttgcgc aacgtcgtgc
     1141 tttcgcagtt gggcgcgcag ccgtggttta tgtagcgcgc ctcgttgccg ttaacgccgg
     1201 cgtcgatcac cgttttcgcg tccaccgtaa acaggaacgt gtgattgtcg ttcggatcct
     1261 tgtcctcgta gcgcgcatcc gcctgcgcgt ggctcacccg ctcgcccagg tactcgatga
     1321 cggtcgtccc cttgcggatg cgccgcgcgg cgaacacgcc gtagccgtgg attggagaat
     1381 gccgaacttc gaacagcgga gtccggcggc gctttggcgg ctttttcata ctgaaatggg
     1441 ggccttgatg gcggcatgag agcggtagtc gaggattgcg aagtcctcga attcgtagtc
     1501 gaacaaactg gcgggcttgc gccggatcac cagtctgggc aagggatatg gggcgcgcgc
     1561 gagctgcagt tctgcctgct ccaaatgatt catatacaga tggcaatcgc cgcccgtcca
     1621 gatgaactcg ccgacccgca agtcgcactg ctgcgccagc atgtgcgtga gcagcgcgta
     1681 agaagcgata ttgaacggca cgccgagcag cgcatcggcg ctgcgttgat acagttggca
     1741 ggacaggcgg ccgttcgcga catagaattg gaacagcgca tgacacggca gcagcgccat
     1801 ctgctgcaac tcgccgacgt tccacgcact caccagaatg cgccgcgagt tcggatcgct
     1861 cttcagctgc tcgacgacgc gtgccagctg gtcgatgttg ccgccgtcgg cgctcggcca
     1921 actgcgccat tgcttgccgt acacgggacc caggttcccg tcctcatccg cccattcatc
     1981 ccaaatgcgc acgccgtgtt cgtgcaaata cttgacgttg gtgtcgccgc gcaggaacca
     2041 cagaagctcg tagatgatgg acggcagatg caatttcttg gtggtcacca gcgggaaccc
     2101 gtcggcgaga tcgaagcgca tctgatagcc gaacaggctc aacgtgccgg tgccggtgcg
     2161 atcctccttg cgggcgccgt agtcccggat attgcgcagg aaatcgagat attgccgcat
     2221 caggcgctca ccgcacccag attgccgctg gccgcgtcgc ggcggtaggc gtaaatcatc
     2281 atcgcggcgc cggcgacgat catcggcatc gtcagtagca tgcccatggt caaccagctg
     2341 ccggccaggt agccgatgtt cgcatcgggc aggcgcaccc attcgacgct gaagcgcgcg
     2401 cagccataga tgagcaggaa cagtccggtc ggcgcgagtc gcggccgggg cttggaagtg
     2461 aaccaccaca cgatgatgaa cagcaccagt ccctcgagac ccgcctcata caattgcgag
     2521 ggatgccggg caatcagctc gccgccggcg ttcggtacgc cgaaacccca aggcagatcc
     2581 gtgggcttgc cccacagctc gccgttgatg aaattgccga gacgtccggc cagcagaccg
     2641 atgccgggga gcggcgcgac gaaatcgagc acgtcgtcca agcgcttgcc ctttacccgt
     2701 ccgaacacga tggcggcgac cagcacaccg ctcatcccgc cgtggaacga catgccgccg
     2761 tcccagatat ggaagacgtt gagccaactc tcggcaatca cgtcatggcc gtaaaaaata
     2821 caccagccga cgcgcccgcc gacgatgacc ccgatggcgc aatagaaaat gaggtcatcg
     2881 atatcgagcg cgctccaggt cgaccccggc cggcgggcgc ggtagcgcgc cagccaccag
     2941 gccgcgacga acccgatcag gtacatgatg ccgtaccaat gcacgcgcag gggcccgatt
     3001 ccgaaaaccg gcccgatcga gatcgcaacc gggttgatgt gcggataggt cagcatgcgg
     3061 ctactttaaa ctctcgttga cgcgacagaa ataggctttg aggtagcggg tctcgggaat
     3121 cgcgggatgc accggatgat ccgccgcctg accgccggtc tggaggattt gcagatgctt
     3181 ctgcgcgctg cgggccgcct tgccgatcgc gtcctgcaat tcttcgggac tgacatggta
     3241 agagcacgcg catgacacga gaataccgtc atcgaccaag acttgcatgg ccagctggtt
     3301 caagcgcttg taggcggcaa gcgccttcgg caggtccttc ttgcgtttgg cgaacgccgg
     3361 tggatcgatg atcacaagat cgtaccgtgc gcgctttttt gcgagatcct ccagcacatc
     3421 gaaggcatct ccgtgcaatg tgtcgagctt gacgccgttg cgttcggcat tggcagcggc
     3481 caactcgagc gccgcggcgg agctgtcgac gcatagcacg tcgcgcgcgc cgctcaccgc
     3541 cgcgcgcacg ccccacgcgc cggcataact gaacacatcc aatactcgcg cgcccttgcg
     3601 cacgtagttg accattgcgc gacgattgcc ggcctggtcg aaaaaccatc cggttttttg
     3661 acccgcggca agcgctgctt gaaattccag accgtcctcc accaccgtat gcaactcatc
     3721 gaagcggccc gtcgcggcct cgatgtaact cggcagcccc tccatctctc gcgccgcgct
     3781 gtcgttcttg aacagcaccg cctcgcactg cagaacctgc tccaaggcgt gttgaatggg
     3841 cagtttcagc cgctccatgc cggcggtccc gatctgcacc acgcaggcag acccgtagcg
     3901 atcgatgacg agaccgggca atccgtcgga ttcgccgtac accaaacgat agtagggtgc
     3961 ggcgtacaac cgctcgcgca atgccagggc cgcgcgcatg cgggtcgcga gccaagcagc
     4021 atcgggcggt tgccacgttt ccagcaggcg cgcactgatc agcgactgcg gattcacgta
     4081 ggcgagaccc aacccccggt cgttgtgcgc caacacacgc accagctcgc caggcttgaa
     4141 tttaccgagc ggcgtctgct gcgtatcgat ttcgttgctg aagatccaca gatgtcctgc
     4201 gtgcagccgg cgatcttcgt tgcgtttcaa gcgcagggcg ggcagctctg cggcacgggc
     4261 ggagtcggac atggacgatt cttaaagtat agtggcgcgc attctaaccg acccggagcg
     4321 ctaacatgca gcgcaatcga ctggccgcgc aaaccagccc ctacctcaag cagcacgccg
     4381 acaatcccgt ggattggtat ccctggggag ccgaggctct gcaagcggcc gagcgcgccg
     4441 acaagccgat tctattgtcg atcggctact cggcctgtca ttggtgccac gtcatggcgc
     4501 acgagtcgtt cgaggatccg gccaccgcgg cgctgatgaa ccgcttgttc gtcaacatca
     4561 aagtggatcg cgaagagcgg ccggacctcg acaagatcta ccaagtggcg caagcattgc
     4621 tcacgcacgg ctccggcggt tggcccctca ccatgttctt gacgccgcgc gatcaatcgc
     4681 ccttcttcgg cggcacctat tttccgaagc aaccgcgtca cggaatgccg gcgttcggcg
     4741 acttgctgat gcgtgtggcc gagtattacc gtacgcactc cgccgagatc gcacagcaaa
     4801 acgcgcaatt gcgcgcggcc tacgccgcgc tcacgccgcc ggccgcggct cgagacgtgg
     4861 cactcgatcg agggccgctg cgcgcggcgc gtgctgcgct cgagcggacc ttcgacgcca
     4921 gattcggcgg cttcactcag gcaccgaaat ttcctcatcc cggcagtatc gaacgctgcc
     4981 tgcggcactg gcatgacacg cgcggcgccg cggcgccgga tctgcaagcg ttattcatgg
     5041 cgagcctcac cttgacgcgc atggccgaag gcggcattta cgatcaactg ggcggcggct
     5101 tcgcgcgcta ttcggtcgac gccgagtgga gcatcccgca tttcgaaaag atgctggacg
     5161 acaacgcgca gctgctgtgt gaatatgcgc gcgcggcgct cgccacgggc gatgcccact
     5221 tcgcacgcat cgccgccgag accgcgcagt gggcgctgcg cgacctgcac tcgctcgagg
     5281 gagggttcta ctcgagcctc gatgcggatt ccgaggggca cgaaggcaag ttctacgtct
     5341 ggacaccggc gcaaattcag gcgctgctga cctctcagga atatgccgcg ttcgcgcgcc
     5401 gcttcggcct cgagcgcggc gccaatttcg aagggcaatg gcatctgcgc gtcactgagt
     5461 ccaacgacga catcgcggtc gcggcgcaca tcgactcggc gcgcgcgaaa ttgctgcaag
     5521 cacggaattt gcgcgtctcg ccggcacgcg atgagaagat cctcacggca tggaacgcgc
     5581 tgatgatcaa ggggctggcg attgccgccc gggtgctgcg tcgcgccgat cttgcgcacg
     5641 ccgcgacggc cgcggttgac ttcgtgcgtc gcaggctgtg gcgcgacggg cgcttgctcg
     5701 cgacatataa ggacgggcgc gcgcacttgc cggcctatct cgacgactat gcctttcttg
     5761 ccgatgccct gctcgagctg ttgcaaaccc gctggcgcag cagcgacttg gagtttgcgc
     5821 aagcattggc gcaggtcctc ctgtcgcagt tcgaagatga cgcggacggc ggattcttct
     5881 tcaccgccaa ggaccacgag cggctcatcc atcgcagcaa gaccttcagc gacgagtccc
     5941 tgccctcggg caacggcgtt gctgccgcgg tgctcagccg ccttggctac ctgctcggcg
     6001 aatcatccta tctcgaggcc gccgagcgca cgttgcgggc gggctggccg atgctcgagc
     6061 agtaccccca ggcccacatg agcctgttga atgcgctcga cgattttctc tccccggttc
     6121 aagtgctgat catccgcggc gatgcgccgc aagccgccca ctggtccgcg cagctcggcg
     6181 ctctgtacgc gccgacacgc atgatcttcg cgattccccg cgacgccgag gcgctgccgc
     6241 cggcgctcgg cgtaaaggcg gctgccgccg gcacggtggc ctatctatgc cgcggcacca
     6301 cctgctccgc cccgttcacc gacttggaag cgattgcgcg cgagctcgca ctgcgcgtct
     6361 agcgcgcagg ttcgctgcac tcgctcaagc gcgcggccaa gcggcgctgc aattcgccgt
     6421 gcaacggctc gcaataccgg ggggcggcag cccagtcgag cgcccacggc tgcagggtcc
     6481 gcgtcgcctc gggctcgctg gcatggcgcg gaaaaatatg tgcatgcaga gcgggatcca
     6541 cattgccgaa cagggcgtag ttgatgcgca agcccgcagt cacggccagc aacgcgtccc
     6601 cgaccaccgc catgtcggac aggaattggc cgcgcagatc gggcgccagc gtgttcaagt
     6661 gcggcacgac gggatcggcg agcaacagac aatatccggt cagcacctgg cgctcgccca
     6721 tcaccaccca cccggagccc aatcgcgcca ccgcggcggg gtaggttccg gcgcggcagc
     6781 gatcgacgat ctcatggatg agcgtcggcg cgctcacggc ccgagcgcct tcgccaccgc
     6841 gtcggcatcc accggcggcg cggaattgcc gaaactcgag cgcaagtaag acaacagcgc
     6901 cgcggcgtcc ttaggtttca gccagccgaa ctgcagcatt tgcgtgctat agcgtcccgt
     6961 cggcatggac ttgggccttt ggcctttgag tacccagcgc gccaactccg ccggatctcc
     7021 caacaccacg ggggacccgg ctaagctggg gtacacgccc gcaatgccgg cgccggtctc
     7081 ttgatgacac ggcaggcaat tgccgttgta aatgcgttgg ccggcatcgg cggcggccgg
     7141 cgcgccgccg ctctgggctg cgccgggagt tttaggggcg cacgccgcga gcgcggcgct
     7201 gagcaaaatg atcgacaata cgttccgcgg taacatctat gctaaatgct accaaaccag
     7261 gaggcgcagc accgacatga tgagactcgc gacgtactgg ctcgccgcgg cggctgcgct
     7321 attcgccgcg gcggcgctgg cgcaggcgcc ggccgaggac gcgctcaagc gcctgatcga
     7381 ggaaggcaat ccggcgtact tgagtgattt ggccagcgcc aacgaccgcg ctgagatcgg
     7441 cgcgctgtat gcggcgaatg cctatcggct gttgtggagc gacggcgcga agccgaccgc
     7501 acaggcgatt gcattgctcc agcaattgcg cctggccggc gaacgcggct tggatccgga
     7561 agactatccc ggcaaccgct tggcttactt gttggtcgac ctgatcgatg cgccgcattc
     7621 gggcatcgag cagtgggctc tgttcgacgc cggactgtcg ctcgccggca tgcggtttct
     7681 atcggacgtg cacatcgggc gagtcgatcc cgccaccatg ggtcgcgacc tcacggtggg
     7741 acgcgtcaaa ctcgatatcc cgacgacgct tgggcacctg gcgagtgcgg tcgacgtgcc
     7801 gctcgccatc gacgcgctcg aaccgcagtt cagccactat gcgcttctca agaagcagct
     7861 gtcgcactac cgtgcgctgg cgctcgagga cggactcacc gctttgccgg cattgccggc
     7921 caagtcgctc aaaatgggcg acacctattc cggcgccgcg caactgcaac gcctgctggt
     7981 cgcgctgggc gatgaggccg cgccggcggc ggctgcaccc gcgccgcaaa ctctgtcccc
     8041 gccgctggtc gatgcgttga agcattttca gagccggcac ggcgaaaagc ccgacggcgt
     8101 gctcggcgcc gcgaccttcg ccgagctgac gcggccgctc ggtgagcgcg tcaagcaaat
     8161 cgaactcacc atggaacgct ggcgctggct gccttcggaa ctcgagactg cccccatcat
     8221 cgtcaacatc ccgcagtttc ggctgtttgc cttcgaatcg acggcggatt ccgaagctca
     8281 cattcggcaa atggacgtga tcgacggcaa ggcattcgac aagacgcaaa cgccggtgtt
     8341 cgccgccgac atgacgtact tgattttccg gccctattgg gaagtcccgt acgacatcgc
     8401 gctcaaggag gtcgtgccga cggcgcgcaa caatccggcc gacatcgagc gccgccaaat
     8461 ggaaatcgtg cgcggtaacg gcgattccgc ggtggtgatg ccgaataccc cggagaatgt
     8521 cgagctggtc gcgcgcggca cgctgcggct gcggcagcgg cccggaccga acaactctct
     8581 cggtctcgtg aagttcctgt ttccgaatcc ctacaacgtg tatctgcact cgacacccgc
     8641 gcagtcgctg ttcgccgagt cgcgccgcga tttcagccac ggctgcgtgc gggtgagcga
     8701 ccctgtcgga ttggccgagt acgtgctgcg cgattc
//
LOCUS       DASWZT010000406         3510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_27431, whole genome shotgun sequence.
ACCESSION   DASWZT010000406 DASWZT010000000
VERSION     DASWZT010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27431"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3223
                     /gene="mfd"
                     /locus_tag="VGE92_11465"
     CDS             <1..3223
                     /gene="mfd"
                     /locus_tag="VGE92_11465"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007966.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEY1314490.1"
                     /translation="ETLPYDGFSAHPDITSARLATLAELPRARRGVWLVAVDTLLQRL
                     APRSYVEAYSLKVHVGETLDLEALRARLALAGYAAVTQVVAHGEFAVRGSLIDVFPMG
                     SETPYRIDMLDRDVDSIRRFDPDTQRSMEKLERIQLLPARETPLNPDAVREFRRRYRL
                     RFTGDLSEQPVYRDVSAGIAPGGIEYFLPLFFDRTSHLFEYLPPDSVLIDMNDAASAV
                     DGLWNGILERHEQLRHDRHRPILDPAEVYLTPAEFLEAYAARRQIQLRTFEWPPGTTG
                     RLRNFPSVAPATVRIDPRAAQPADELIAHIQASPARILLAAESAGRRELLRELLQARE
                     IQAKIFENFAAFVASSARLGIAVSPAVSGLRLEAPALEILTETQLFGDRARQERRRRR
                     AERDPAKILKELSDLRVGAPVVHESYGVGRYVGLQTMDVAGYTGEFLVLEYADGDKLY
                     VPVQALHLVSRYTGAPAETAPLHKLGGEQWQKARRKAAQRIRDVAAELLDLYSRRAAR
                     QGTRMLAGEAEYRAFQAGFPFEETADQAAAIEQVLTDLKSDKPMDRVICGDVGFGKTE
                     VALRAAFVAVQAGKQVAVLVPTTLLAQQHHTTFVDRFADWPVRIESLSRFRSNKEADT
                     VIAGVAAGTVDIVIATQRLLQGKVRFKDLGLVVIDEEHRFGVRDKEKLKALRTEVDVL
                     TLTATPIPRTLNMAMGGLRDLSLITTPPAERLAVKTFVLEWNETVVREASLREIRRGG
                     QIYFVHNTIETIEKTAQAIRELVPEATVAVGHGQMRERDLEQLMLDFYHRRFNVLVCT
                     TIIESGIDVPTANTIVIDRADRFGLAQIHQLRGRVGRSHHRAYAYLITPPRKAMTADA
                     IKRLEALESLEELGAGFTLATHDLEIRGAGELLGDEQSGQIQEIGYNLYMELLERAVA
                     ALKSGKQPDLERPLDVGPEVELHLPMLIPEDYVPDVHLRLVLYKRIAGAASREELDEL
                     KVEMIDRFGPLPEYALSLFRATRLKLRAAELGIRKIDAAAGSGYFIFEEANKVDAQRV
                     LKMIQGKPKEYRLDGPLKLRFTHDARNEEKLFTRIEQLVEQLA"
     gene            complement(3262..>3510)
                     /locus_tag="VGE92_11470"
     CDS             complement(3262..>3510)
                     /locus_tag="VGE92_11470"
                     /inference="COORDINATES: protein motif:HMM:NF020909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 family protein"
                     /protein_id="HEY1314491.1"
                     /translation="LQARIAKTVAFLKSVNEGQMKDAETRSIELKFPNGSMKFKGLDY
                     LTGFVLPNFYFHISMVYALLRKSGVAIGKSDFLGDPPK"
BASE COUNT          683 a         1124 c         1085 g          618 t
ORIGIN      
        1 ggagaccctc ccctacgacg gcttctccgc gcatccggat atcacctccg cgcgtctcgc
       61 gacattggcc gaattgccgc gcgcgcggcg cggcgtctgg ttggtcgccg ttgacacgct
      121 gctgcagcgc ctggcgccgc gcagctacgt cgaggcctac tccctcaagg ttcacgtggg
      181 cgagacgctc gacttggaag cgctgcgcgc tcgattggcc ctcgccggct acgccgcggt
      241 cacgcaggtc gtggcgcacg gcgaattcgc ggtgcgcggc tcgttgatcg atgtatttcc
      301 aatggggtcg gagactccgt atcgaatcga catgctggat cgcgatgtcg acagcatacg
      361 gcgcttcgat ccggacacgc agcgctcgat ggagaaactc gagcgcatcc agctgctgcc
      421 ggcgcgcgag acgcccctca atcccgacgc ggtgcgcgag tttcgacgcc gttaccgctt
      481 gcgcttcacc ggcgatctgt cggaacagcc ggtgtatcgc gacgtcagcg ccggcattgc
      541 accgggcggc atcgagtatt ttctgccgct gttcttcgac cgcacctcgc atctgttcga
      601 atatctgccg ccggacagtg tgctcatcga catgaatgat gccgcatccg ccgtagacgg
      661 gctttggaac ggtatcctcg aacggcacga acagttgcgc cacgaccgcc accggccgat
      721 cctggacccg gcggaagtgt atttgacgcc cgcggaattc ctggaggcct acgcagcgcg
      781 gcggcagatt caactacgca cgtttgagtg gccgccggga acgaccgggc ggttgcgcaa
      841 ttttccgagc gttgcgcccg caaccgttcg catcgaccca cgcgccgcgc aacctgcgga
      901 cgaattgatc gcgcacatcc aggcgagccc ggcgcgcatt ctgctcgccg cggaatcggc
      961 cggacgccgc gagttactgc gcgaactgct gcaagcacgc gaaatccaag caaaaatctt
     1021 cgagaatttc gcggccttcg tcgccagttc ggcgcgactc ggaatcgccg tctcgccggc
     1081 cgtatcgggc ttgcggctcg aagcgccggc gctcgaaatc ctcaccgaaa ctcaattgtt
     1141 cggcgatcgc gcgcggcagg aacgcaggcg ccgccgcgcc gagcgcgacc cggcgaagat
     1201 actcaaggag ctgtcggacc tgcgcgtcgg cgctcccgtg gtgcatgaat cctacggcgt
     1261 cggccgctac gtcggactgc agaccatgga cgtcgccggc tacaccggcg aatttctcgt
     1321 gctcgaatat gcggacggcg acaagctcta tgtgccggta caagcactgc acctggtgtc
     1381 gcgctacacc ggggcgccgg ccgaaaccgc gccgctgcac aagctcggcg gcgagcaatg
     1441 gcagaaggcg cgccgcaaag cggcacagcg catccgcgat gtcgccgccg aactcctcga
     1501 tctgtattca cgccgcgccg cgcgccaagg cacgcggatg ctggccggcg aggccgagta
     1561 tcgcgccttt caagcgggct tccccttcga ggagacggcc gaccaagccg cggcgatcga
     1621 gcaggtgttg accgatctta aatccgacaa gcccatggat cgggtcattt gcggcgacgt
     1681 cggtttcggc aaaaccgagg tcgcgttgcg cgccgcgttc gttgcggtac aggccggcaa
     1741 gcaagtcgcc gtcctcgtac cgacgacgct cctggcccaa cagcaccaca ccactttcgt
     1801 cgaccgattc gccgactggc cggtgcgcat cgagagtctg tcgcggtttc gcagcaacaa
     1861 ggaggccgac acggtaatcg ccggtgttgc cgccggcacg gtggacatcg tcatcgccac
     1921 tcaaagattg ctgcagggaa aggtgcgctt caaggatctg ggcctggtgg tgatcgacga
     1981 agaacaccgt ttcggcgtgc gcgacaagga gaaactcaag gcgctgcgca ccgaagtcga
     2041 cgtgctgacg ctcaccgcca cgccgatccc gcggaccttg aacatggcca tgggcgggct
     2101 gcgcgatttg tcgttgatca cgactccgcc ggcggagcgg ctggcggtga agaccttcgt
     2161 tctcgaatgg aatgaaaccg tggtgcgcga ggcgagcctg cgcgagattc ggcgcggcgg
     2221 gcaaatatat ttcgtccaca acacgataga aaccatcgag aaaacggcgc aggcaatccg
     2281 cgagctcgtg cccgaagcaa cggtcgcggt gggtcacggc cagatgcgcg aacgcgatct
     2341 cgagcagctg atgctcgatt tttatcaccg gcgcttcaat gtcctggtgt gcacgacgat
     2401 catcgagagc gggatcgacg tgccgaccgc gaacacgatc gtcatcgacc gcgcggaccg
     2461 atttggactg gcgcagattc accaactccg aggcagagtc ggacgatcgc atcaccgggc
     2521 ctatgcgtac ttgatcacgc cgccgcgcaa ggccatgacg gcggacgcca tcaaacgtct
     2581 ggaagcgctg gagtcgctcg aggaactcgg cgccggattt accttggcga cccacgacct
     2641 cgaaattcgc ggcgcgggcg aacttttggg cgatgagcaa agcggacaga tccaggagat
     2701 cggctacaac ctctacatgg agctgctgga acgcgcggtg gctgccttga aatccggcaa
     2761 gcagcccgat ctcgaacggc cgctcgacgt gggtccggaa gtcgaactgc acttgccgat
     2821 gctgatcccc gaagactatg ttccggacgt gcacttgcgc ttggtgctct acaaacgcat
     2881 tgccggcgcc gcctcgcgcg aggaactcga tgaactgaaa gtcgaaatga tcgaccgctt
     2941 cgggccgctg cccgagtacg cgctatcgct atttcgcgcg acgcggctca agttgcgcgc
     3001 cgcggaactc ggaattcgca agatcgacgc cgcagccggc agcggctact tcatattcga
     3061 ggaggccaac aaagtcgatg cgcagcgggt cctcaagatg attcaaggca agccgaagga
     3121 atatcgcctc gacggccccc taaaattgcg tttcacccat gacgcccgca acgaagaaaa
     3181 attattcacg cgcatcgaac aattggtcga acagctcgcc taacgccacg tcgccgcgcc
     3241 cgcaccgttg aatcgcggcc gttacttggg agggtcgccg agaaagtcgc ttttgccgat
     3301 cgcaacgccg gatttgcgca atagcgcgta caccatgctg atgtgaaaat agaaattcgg
     3361 cagtacgaaa ccggttaagt aatccagccc cttgaatttc atgctgccgt tcgggaactt
     3421 gagttcgata ctgcgtgttt ccgcgtcctt catctggcct tcattgaccg acttgaggaa
     3481 agccaccgtc ttggcgatgc gcgcctgcag
//
LOCUS       DASWZT010000407         4411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_75679, whole genome shotgun sequence.
ACCESSION   DASWZT010000407 DASWZT010000000
VERSION     DASWZT010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75679"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VGE92_11475"
     CDS             complement(<1..513)
                     /locus_tag="VGE92_11475"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1314492.1"
                     /translation="MAFESASITPAAGADSSEAHATYSTNLQVFVYVLFFAFGGITSL
                     NDVLIPKLKALFTLGYGEVMLVQSAFFVAYFIISIPAAALVRRFGYMRSAVVGLLTMT
                     VGCLLFIPASSFGLFGLFLLALFVLASGITTVQVVANPLISMLGPARTASSRLTFAQA
                     FNSLGTTLFPY"
     gene            complement(569..1861)
                     /locus_tag="VGE92_11480"
     CDS             complement(569..1861)
                     /locus_tag="VGE92_11480"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEY1314493.1"
                     /translation="MGKNLVIIGLQWGDEGKGKVVDLLTDQVQAVVRFQGGHNAGHTL
                     VIGGKKTILSLIPSGILHPHVQCLIGNGVVLSLEALFKEAQMLIDEGVPVFERLRVSP
                     ACPLILASHVALDRAREKAQGAKAIGTTGRGIGPAYEDKIARRAVRVADLFHRERFAA
                     KLGEILDYHNFILQRYFQTAAIDFQQVLESNLELGERLKPLVADVTGILEQLSVDGRN
                     VLFEGAQGAMLDVDLGTYPYVTSSTTTAGGAAPGTGIGPRRLHEVLGIVKAYATRVGA
                     GPFPTELFDEFGEYLSRVGHEFGSVTGRRRRCGWFDAVALRRSIVHNSCSLLCVTKLD
                     VLDGLDTIRICVGYKIEGDVTTSPPLLSEHFGAAEAVYEDMPGWRENTVGITRYDDLP
                     RNARGYLERLQILVGVPITLISTGPDRDQTIIRQNPFG"
     gene            complement(1854..2069)
                     /locus_tag="VGE92_11485"
     CDS             complement(1854..2069)
                     /locus_tag="VGE92_11485"
                     /inference="COORDINATES: protein motif:HMM:NF021366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HEY1314494.1"
                     /translation="MMEIGWSELERYLGGAFALYLVIEGVLPFVNPALAQRLMAKLAS
                     AEPTQLRWGGLISMVAGLALLWMVRNG"
     gene            complement(2066..2947)
                     /locus_tag="VGE92_11490"
     CDS             complement(2066..2947)
                     /locus_tag="VGE92_11490"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEY1314495.1"
                     /translation="MQNRILYILLALVAVILVVRASVFTVSEGQLAIKSIGGEIVDAS
                     FAPGLHFRIPLVEEVSRFDKRIITQLYRQELFLTREQEQLKVDLYVKWRINNLRRYYE
                     ATGGTEEVANDRLGETVKDSIKTVVTQRTLQQVVTAERTEFTDSMMKNARPAAEQMGV
                     DLVDVRITKIDLPQQVQDSVFERMRATFKAQAAKLRAEGVESSERTRADANKQQTEIL
                     ADAARQADQIRGEGDASASEVYAKVYSKNAEFYSFYRSMQSYREAVGTQGDVLVIAPD
                     SAYFKYFNKSQPQPPAR"
     gene            complement(2947..4086)
                     /gene="hflK"
                     /locus_tag="VGE92_11495"
     CDS             complement(2947..4086)
                     /gene="hflK"
                     /locus_tag="VGE92_11495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEY1314496.1"
                     /translation="MAWNEPGGEKRNPWNRPQQSQNDLDDVLRNFQRRLSALFGRGGA
                     GSGGGVGPSSASFGKGLSSVLILIFAVWIGSGVYRVDAQERAVVLRFGKYLQTTGPGY
                     NWHLPWPIESKSIVNVSHQYSVVDDASMLTADTNLVEVKSAVQYIQPDALKFLFKVRD
                     VEDTLTQVSESAMREAVGQASLDKALAYDVSITARAKFLLQRTLDSYDMGIQILGVKL
                     TGVNVPEAVQDAQRDAIKADKDRQRYQQDAETYRNDVLPRARGAAAKEVLDAEAYRLQ
                     VLALAQGDTSRFDQVLTQYEHAPAVTRERMYLEAMENVYKNSRKVIVDTKNGNSMMYL
                     PLDKLIAPGPQSISMPNDTMTVPPARIPEIQVAPPEDPARARGVR"
     gene            complement(4150..>4411)
                     /locus_tag="VGE92_11500"
     CDS             complement(4150..>4411)
                     /locus_tag="VGE92_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314497.1"
                     /translation="LPLLREALARAVRANQVRRTLHLELKAAAVRSDLYGRNAVRCER
                     QCEDGSWDLEVELDLSEVAKLLGNPGVTVVETSERTREQRVA"
BASE COUNT          854 a         1414 c         1300 g          843 t
ORIGIN      
        1 gtagggaaac agtgtcgtac ccagggaatt gaaggcctgc gcgaaggtca accggctgct
       61 cgcggtgcgc gccggtccca gcatggaaat taatgggttc gccaccactt gcaccgtggt
      121 gatgccggag gccagtacga acaaagccag cagaaacagc ccgaacaacc cgaacgagga
      181 tgccggtatg aacagtagac accccaccgt catggtcagc aggccaacca ccgccgagcg
      241 catgtagccg aagcgtcgca ccagcgcggc ggccgggata gaaatgatga agtacgccac
      301 gaagaacgcc gattgcacca gcatcacctc tccgtacccc agcgtgaaca acgccttgag
      361 tttcgggatg agcacatcgt tgagactggt gattccgccg aaggcaaaaa acaacacgta
      421 cacgaacact tgcagattgg tcgagtaggt ggcgtgagcc tcgctgctat cggcaccggc
      481 ggcaggggtt atggaggcgg attcaaatgc cattgtgcgt acccttgtat tgcagtcgca
      541 gcatcttacc cgatcggcaa tcaggagatc agccgaacgg attctgtcga atgatggtct
      601 ggtcgcgatc cgggccggtg gagataagcg ttatcggcac gccgaccaga atctggaggc
      661 gctccagata gccgcgggcg ttgcgcggaa ggtcatcgta acgagtgatg ccgaccgtgt
      721 tctcgcgcca ccccggcata tcctcgtaca ccgcttcggc tgcgccgaaa tgctccgata
      781 gcaacggcgg cgaggtcgtc acgtcgccct cgatcttata gccgacgcaa atgcgaatcg
      841 tatccaggcc atccagcaca tcgagtttgg tgacgcacaa caacgagcag ctgttgtgaa
      901 cgatggagcg ccgcaaagcg accgcatcga accacccaca gcgccgccgc cggccggtca
      961 cggaaccgaa ttcgtgaccg acacgcgata aatattcacc gaattcgtca aacagctcgg
     1021 tcggaaacgg tcccgcgcca acccgcgtcg cataggcttt gacgataccg agcacttcgt
     1081 gcagacggcg cggaccgatg ccggtgccgg gggccgcgcc gccggcggtg gtggtggatg
     1141 aggtgacgta cggatacgtg ccgaggtcca catcgagcat cgcgccttgc gcgccctcga
     1201 acagcacatt tcgcccatcg acgctcagtt gctcgagtat tccggtcaca tccgcaacca
     1261 gcggcttcaa gcgctcgccg agttccaggt tcgattccaa cacttgctgg aaatcgatgg
     1321 ctgcggtctg aaaataacgc tgcaaaatga agttgtgata gtcgagaatc tcgccgagct
     1381 tcgccgcgaa gcgctcgcga tgaaatagat ccgcgacccg caccgcgcgg cgcgcgatct
     1441 tatcttcgta cgccgggccg atgccgcggc cggtggtgcc gatggctttc gcgccctgcg
     1501 ccttctctct ggcacggtcg agcgccacat gcgaggcgag aatcaacggg catgccgggc
     1561 tcacgcgcaa tcgctcgaac accggaactc cctcatcgat cagcatctgc gcttccttga
     1621 acagcgcctc gagcgacagc accacgccgt tgccgatcag gcactgcaca tgcgggtgca
     1681 gaatgcccga cggaatcagg gacaagatgg ttttcttgcc gccgatgacc aaggtgtgtc
     1741 cggcgttgtg tccgccctga aaacgcacca cagcctgcac ctggtcggtc aacagatcga
     1801 ccaccttgcc cttgccctcg tcgccccatt gcaagccgat gatgaccagg ttcttaccca
     1861 ttgcgcacca tccacagcag cgccaatccc gcgaccatgc tgatgaggcc accccagcgc
     1921 aattgcgtgg gctcggcact cgcaagtttt gccatgagac gctgcgcgag ggccgggttg
     1981 acgaacggca atacgccctc gatcaccagg tacagggcaa aggcaccgcc caggtagcgc
     2041 tcgagctcac tccaaccgat ctccatcatc gggccggggg ctgcggctgc gatttgttga
     2101 agtacttgaa gtaggcgctg tccggggcaa tcaccagcac gtcgccttgg gttcccaccg
     2161 cctcgcgata cgactgcatg ctgcggtaga aactgtagaa ctctgcgttc ttcgaataaa
     2221 ctttggcata cacctcgctg gcggaggcat cgccctcgcc gcgaatctga tctgcttggc
     2281 gcgccgcatc cgcgaggatc tccgtctgct gcttgttggc gtccgcgcgg gtgcgctcgg
     2341 aggattcgac gccttcggca cgcagcttcg ccgcctgcgc cttgaaagtc gcgcgcatgc
     2401 gttcgaacac cgaatcctga acttgctgcg gcaggtcgat cttggtaatg cgcacatcga
     2461 ccagatcgac acccatctgc tcggccgccg gccgggcatt cttcatcatg gagtcggtga
     2521 attccgtgcg ctccgcggtg accacctgtt gcagcgtgcg ttgggtcacc acggtcttga
     2581 tgctgtcctt gacggtctcg ccgaggcggt cattggcgac ctcctcggtg ccgccggtgg
     2641 cttcgtagta gcggcgcaaa ttgttgatgc gccatttcac gtataaatcc accttcagct
     2701 gctcctgctc gcgagttaga aaaagctcct gccgatacaa ctgggtaatg atgcgcttgt
     2761 cgaagcggct gacttcctcg accaggggga tcctgaaatg cagacccggg gcgaagctgg
     2821 cgtcgacgat ttcgccgccg atcgacttga tggcaagctg gccttcgctc accgtgaaga
     2881 cgctggcgcg gaccaccaga atgacggcga ccagcgccaa caagatgtag aggattcggt
     2941 tttgcatcag cgcaccccac gcgcgcgcgc gggatcttcg ggcggagcca cttgaatctc
     3001 ggggatccgc gccggcggca ccgtcatcgt gtcgttcggc atggaaatcg actgcggccc
     3061 gggtgcgatc aatttgtcga gcggcaagta catcatgctg ttgccgttct tggtgtcgac
     3121 gatcaccttg cgcgaattct tgtacacatt ctccatggcc tcgagataca tgcgctcgcg
     3181 cgtgaccgcg ggtgcatgct cgtactgcgt cagcacctga tcgaaacgcg aggtgtcgcc
     3241 ctgcgccagc gccagcacct gcaggcggta ggcttccgcg tcgagcacct ccttggcggc
     3301 ggcgccgcgc gcccgcggca gcacgtcgtt gcgataggtc tccgcgtcct gctggtagcg
     3361 ctgccggtcc ttatccgcct tgatggcgtc gcgctgtgcg tcctgcaccg cctcgggaac
     3421 gttcacgccg gtgagcttga cgcccaggat ttgaattccc atgtcgtagc tatcgagcgt
     3481 ccgctgcagc aggaatttag cgcgcgccgt gatcgacacg tcgtacgcca gagctttgtc
     3541 gagcgatgcc tggccgaccg cctcgcgcat tgcgctctcg ctcacctgcg tcagcgtatc
     3601 ttcgacgtcg cgcactttga acaggaactt gagcgcatcg ggctgaatgt attggaccgc
     3661 ggatttgact tccaccaaat tggtgtcggc ggtgagcatg ctggcgtcgt cgaccacgct
     3721 gtattgatgc gatacgttga cgatggattt ggattcgatc ggccagggca gatgccagtt
     3781 gtagcccggc ccggtggttt gcaaatattt tccgaagcgc agcaccaccg cgcgctcctg
     3841 tgcgtcgacg cggtacacgc cgctgccgat ccacaccgcg aagatcaaaa tcaggacgct
     3901 gctcaatcct ttgccaaagc tggccgaact cggtccgacg ccgccgccgc tacccgcccc
     3961 gccccgcccg aacagcgccg acagccgccg ctggaaattg cgcagcacat cgtccaagtc
     4021 gttctgcgac tgctgcgggc ggttccacgg attgcgtttc tcgccgccag gttcgttcca
     4081 agccatgcct agtgcctata tatgaagagc cgcgcattct aacagccgat gagccaccca
     4141 caagagagcc taggcgacgc gctgttcgcg ggttctttcg ctagtttcca ccacagtgac
     4201 cccggggttg cccaataatt tcgcaacctc ggataagtca agttccacct cgaggtccca
     4261 gctgccgtcc tcgcattgcc gctcgcaacg caccgcattg cgcccgtaca gatccgatct
     4321 gactgcggcc gccttgagct cgaggtgcag ggtgcgcctc acctgattcg cccgtaccgc
     4381 acgtgccaac gcctcgcgca acagcggcag g
//
LOCUS       DASWZT010000408        17711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_61251, whole genome shotgun sequence.
ACCESSION   DASWZT010000408 DASWZT010000000
VERSION     DASWZT010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 17711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17711
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61251"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VGE92_11505"
     CDS             complement(<1..661)
                     /locus_tag="VGE92_11505"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY1314498.1"
                     /translation="MRLPATLAATVKVSANSSMISAQQILATYALIRPHIRRTPTLVV
                     DAADFGIDAEPLTLKLESLQHAGSFKVRGAFTHLLTRNIPKVGVVAASGGNHGVAVAY
                     AAKKCKVPAKVFVPTVAASTKLKQIADCGADLVVSGERYADALAASEAWAAQTGALRI
                     HAFDQEETLRGQGTVGLELEEQAGGITTLLVAVGGGGLIAGISAWYAGRIKIVGVEPE
                     LA"
     gene            complement(615..2513)
                     /locus_tag="VGE92_11510"
     CDS             complement(615..2513)
                     /locus_tag="VGE92_11510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY1314499.1"
                     /translation="MLLIAALLSLSAQADTGSSAQAPYSAVSRQLNVMVRARDGISLA
                     TDIYRPANGGAAVTQRLPVLLHRTPYDKSEAATVAIAETLAEHGYVVMVQDTRGRHRS
                     QGVFVKYYDYDAYDGYDTIEWAAGQPYSNGKVGMYGTSYAAHTQADAAKLNPPHLNTL
                     VINMGGMSNAWDHSVRYDGAFEMGRQLTWAWSQALEDAKDPITQALLSREKVTDWYSV
                     LPLRKGLSPLAIAPTYEGYYLDEATQSDYDAHWQTLGMQWQKYYAQTADIPMLHVGGW
                     YDIYLRGTIENFRSLSAIKKSPIQLLIGPWTHHGNTADYAGDVAFGSAAAISDFDTAF
                     HLRWFDYYLKGRKTSAVERAPVQYFLMGTGDGHKDERGRLVHGGEWREAKQWPPAAAQ
                     RTAFYLHGDGSLSGEAALPTEPASTTYHFDPGHPVPTIGGGVSKRLADGAYDQRERPD
                     MPGSRAPFLPLRARSDILVFETAPLPSDVAVAGPVEITLFAASSGVDTDFTAKLIDVY
                     PPSESFPQGFDMNLTDGILRASYRDASPTRRLLAPGQVYSMLIRPFDTANVFKKGHRI
                     RLDISSSNFPRFDVNPNTGEALGENRRQIAVDNTVYHDPAHRSALALWLLPREVTRYT
                     GGNRQGER"
     gene            complement(2543..3052)
                     /locus_tag="VGE92_11515"
     CDS             complement(2543..3052)
                     /locus_tag="VGE92_11515"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEY1314500.1"
                     /translation="MPTATLRAMGLSVLLLMVSLVARGDLKRAKVDEGQYFSAAQLAA
                     QVAHPVDGFDFKAVSSRPESTILIARRDKTGEVEVHAALDDILLVESGRATVTVGGRV
                     TGNRQTEANEWRGGEQSGGREYAVAAGDLLLIPAGSPHLVTVAANAPFTYLIVKIPAQ
                     HPLTASNGP"
     gene            3273..3761
                     /locus_tag="VGE92_11520"
     CDS             3273..3761
                     /locus_tag="VGE92_11520"
                     /inference="COORDINATES: protein motif:HMM:NF022458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2844 domain-containing protein"
                     /protein_id="HEY1314501.1"
                     /translation="MAMNGSKTRGALMPSLVSMLVAAVVGLCLAPAARAGLGETAASV
                     QRDHTALRGTSLTVTPMASYDLHEITSPDSRVREYVSHAGTVFAVTWSGRARPDLSVV
                     LGARYAEYSKAAATHRGSHKVFALSSDDLVMHVTKLPRGFEGEAHAPTLFPAGVSPQD
                     IR"
     gene            3778..5310
                     /locus_tag="VGE92_11525"
     CDS             3778..5310
                     /locus_tag="VGE92_11525"
                     /inference="COORDINATES: protein motif:HMM:NF023352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3443 family protein"
                     /protein_id="HEY1314502.1"
                     /translation="MTNSIHRAAWLSCALFLAACSGGSNAPGVTLTSIAATPLSIPLS
                     VGGTQALTVTGTYSNATTATLASGLTFASDNAAVATVSNAGLVTAVADGTAHITVTDS
                     AASLSATPVTVSVTAAASTANTLAVVVDSGPAALLAENPPEVDANVLFATVTICTPGS
                     LTACQSIDHVQVDTGSIGLGIMSGVLNGAAVPQPIQVSGGPLRECIAYADGYVWGSMA
                     TVDVQIGGRTISSLPVNIMGDPAAGTAPASCTSGYTSSQNESSVLAFGANGVLGIGVF
                     LQDCGIACTTEPAPTQAYYACPTSGCVAAAAALNQQMNNPVGALSADNNGVVIVLPAV
                     TSTGQVSVSGTLYYGIGTQANNALGSSTQLLTVLDSGTTAGTLTTTFNGSTLAGSVID
                     AGSSGYFFNDSSLPACSDQPAYYCPVSGSAAATVSFNATISGENGITSPQVFTIDNAD
                     SLFSSSQTLTAFPTLGGPNGALNGIIPGFDWGLPFFYNRSVYVLFETYAVGGTTGPAI
                     GF"
     gene            5504..6004
                     /locus_tag="VGE92_11530"
     CDS             5504..6004
                     /locus_tag="VGE92_11530"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEY1314503.1"
                     /translation="MKSLRPLMRPISCALILAASVAAGTYANAQATTGAATAAPSGHH
                     WRHRQFGPMHVLHKLNLTAEQETQIKSIIEQARAQAKTEMESEHANHESLASTAPTDA
                     NYPALLAAAKTNAANRVQRMSEVWEQAYAVLTPAQKAQIPAILAAEKQQMEARKAAWQ
                     ASHPQS"
     gene            complement(6660..6821)
                     /locus_tag="VGE92_11535"
     CDS             complement(6660..6821)
                     /locus_tag="VGE92_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314504.1"
                     /translation="MVGSQASGRAQAVTLSKAPGELGVVVDASVLDADVAALDAEAAS
                     VLDGDPPPP"
     gene            6857..7648
                     /locus_tag="VGE92_11540"
     CDS             6857..7648
                     /locus_tag="VGE92_11540"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY1314505.1"
                     /translation="MVATMDEVFKALADATRRKLLDKLFEKDGQTLQALENRLPMTRF
                     GVMKHLKLLEAAGLIVTRRQGREKLHFLNAVPIRLIHDRWVDKFTAPWVAGLSDLKHR
                     LEGQTMQKVFEIYIKTTPARLWKALTDTEMRRKYTFGAEVTSEWTPASRYHGVGHGAP
                     IFEGENLEVDPPRRLVQSFRALWGEDVKSEGTSRVTWEIEPVGDSCLLKVTHDQLRED
                     ANTQLYGGWPMVLSGIKTLLETGEMLTTPGSLRWVQGAQGQSSIA"
     gene            7709..8392
                     /locus_tag="VGE92_11545"
     CDS             7709..8392
                     /locus_tag="VGE92_11545"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY1314506.1"
                     /translation="MYELYIANKNYSSWSLRPWVLMRELGIAFREHLLPFGQESLWSA
                     FRKISPAGKVPCLIDGETVVWDSLAIVEYLAESHPGVWPGTAAARAWARSAAAEMHSG
                     FGELRSRCSMSCGQRIKLHEFPPALERDVARIDTLWGDGLQRFGGPFLAGAAFTAVDA
                     FYAPVAFRVQGYGLSLGSAAAAYVNRLLETRAMREWYADALKETIRDQPHEEDVARMG
                     RVLEDSRAK"
     gene            8389..9177
                     /locus_tag="VGE92_11550"
     CDS             8389..9177
                     /locus_tag="VGE92_11550"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEY1314507.1"
                     /translation="MKAVGFQLLAADEPPPFLELGRRGQSNFVIIVDHAGSRIPRRLN
                     NLGLPASELQRHIAWDIGALSVARQMAAALDAPLLAQNYSRLVIDCNRDPKVASSIPQ
                     SSEAIEIPGNRNLSEPEVLARRAEIFEPYHERVRALLDERAAANQTTILVAQHTMTDV
                     YHGERRAMHAAVLYNRDRRFAGLVLDSLRRDGALNIADNEPYFVSDETDYSIPRHGEA
                     RALPHIEIEIRQDLVSEAAGQTQWAQRITAALREAERVFLAAPA"
     gene            9338..10432
                     /locus_tag="VGE92_11555"
     CDS             9338..10432
                     /locus_tag="VGE92_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314508.1"
                     /translation="MYAAVLALFSLVGAWVAWRRNPSYTARSTLRTLLVTALAIAVLI
                     GMIVVAVNFSMNRSAPVAYATMATVVVVGSLSMIFILQAASTPKDAKLITALPASAKI
                     LHVHRQKVYHWSKVFAVIIAVCGLLALLIPGDAKYIPLAFGGMALLLASILLPVLYAT
                     TRTADRSLTALELNPWVHWRYTPAQWQQWTEVQVGRMKATPPTFILKRDWHKLAWAFG
                     AIAAGVGIFSPGTWLEKGLYVICVCGAITALVMLSARSQHRMPEMNAAKLAKIEPEVY
                     FGHDGLYCDGGYLTWLGLSVYLVAASVDTRAPRSLAFNFEKVIPNPYGATVPLQIHQS
                     VLIPEGADADIARLQRELSGRCPKAHIALA"
     gene            complement(10444..11073)
                     /locus_tag="VGE92_11560"
     CDS             complement(10444..11073)
                     /locus_tag="VGE92_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314509.1"
                     /translation="MSDNPAPRPRLERWLALLVRYWAFNMTLIHDQGTNWPGFGYTPE
                     EKTQLQSIARPVPGIEYFVWLALTVIFFMLILSAVVIAGMNSLPAQAAAPLFFLQLGL
                     DLIISLSVGFPAAMVPAAALAGRWFKVSDAALPERAATAHYFHKLWFQITRIALIGVL
                     VLIPLWIFAPGDSKVWAVGQWVLPLLSPAVAALTAAYYFSGRLRRSAGS"
     gene            11156..11827
                     /locus_tag="VGE92_11565"
     CDS             11156..11827
                     /locus_tag="VGE92_11565"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEY1314510.1"
                     /translation="MCDRYVIPDQVAAEREFLPAQVWWKFEPHFNVAARRYVPAIRVH
                     DAQTEGMMMRWGLIPAETKGKPSGSSRACADIERVERSKTYKMPWLNGQRCILPAAGF
                     FVWKLTPANYRQPYYVRVNNRSVFGFAAVWDRWSGEDEDVIESCSIIRVPANSLLADV
                     ADSDRMPAVLKRRDYDTWLRGTPVEARAALQSYNPEWMTAYAVSPRINSQEPDDAGLI
                     QPIDA"
     gene            11824..12432
                     /locus_tag="VGE92_11570"
     CDS             11824..12432
                     /locus_tag="VGE92_11570"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEY1314511.1"
                     /translation="MRWIGLLLICAAACGAAAAQDVALITAAGQRQMLASPGTPGVGA
                     QKPDVVIVEYFDYNCPYCKKLVPTLQALLAQDPKIGIVYKDWPILGPVSAYAAAAALA
                     AGWQGKYLAAHDALIVGPRLAANTQVDALLQRAGVDLGALKKDRTRHAKEIDALLARN
                     DQEAHALSLDGTPGIVVGRLLVPGIAGVDDLKQLIARSRQSK"
     gene            complement(12442..13503)
                     /locus_tag="VGE92_11575"
     CDS             complement(12442..13503)
                     /locus_tag="VGE92_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314512.1"
                     /translation="MAKFDLRGLSLSAAAALLLIPFAGHCADSPAAAQTEFGGQCAEA
                     LAEGKHQATDCKITWTDKDGKTYCFSNDAAKKTFLASPAENLQRARAFMAAGSVEDTE
                     KAMQDFTGSDAETLVKGFIADKLKANNGLFPFDDPLNGDHLKLNYDDVDFTRTIDGYG
                     FFPDVKFHDSQDAQKKYLVDFWVTPVAGKLQIQETRIYKEPLKSDGKWVTMARQPIPW
                     WWIPASEHPGHLATKRGWEVMSAVEQRAVQDSLKNNGVFKLKDDKTGKELELEFVDTH
                     QPVRQLDNDGHYFACTDFRVVGTKDQIYDIDFWVSDKDGSMVVDQMKVHKVPELKSGQ
                     WIQVPRYEWKDVGASHVVP"
     gene            complement(13527..14510)
                     /locus_tag="VGE92_11580"
     CDS             complement(13527..14510)
                     /locus_tag="VGE92_11580"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEY1314513.1"
                     /translation="MVNPKNPQWPELPVSAWADTRATLQLWTQIVGKVRLMQTPWLNH
                     SWHVALYVSTKGLTTSPIPHGARSFEIEFNFLEHTLDITLSDGNTRRLPLRPQSVADF
                     YAVVMTALADLGVPVAINEHPCEIANCIPFSQDRTHAAYDPNYAHRFWQVLLQTDRVL
                     KRFRTGFLGKCSPVHFFWGSFDITVTRFSGRRAPTYPGTAPGVTAAVMQEAYSHEESS
                     AGFWPGGNGIDYPAFYSYAYPSSARFGEIAVQPAGAFFSAQLGEFLLPYEAVRTAADP
                     DAALLAFLQSTYEAAAVSADWDRAALECPRGVPGVPRLPQGIDFHGQSAYT"
     gene            14606..15715
                     /locus_tag="VGE92_11585"
     CDS             14606..15715
                     /locus_tag="VGE92_11585"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HEY1314514.1"
                     /translation="MRRAHYLTGILLAMTGAGAGAATVTSEQALAMDPAAAARFAGLA
                     LKCLHAEYPNHISHTLDSDADALAPHVLYPAFYGCFDWHSDVHGHWLLVRLLRVLPEA
                     PFAKQARTELARSLTPENIGGEVAYLKRAGRASFERPYGLAWLLKLSAELRLWNDAQG
                     REWAGALRPLETEAAARLKSWVPKLYYPIRIGEHDQTAFSFGLIWDWAAVAGDAGMRD
                     VLKDAAQRFYLKDRHCPLDYEPSGEDFLSPCLAEADFMRRVLDRAEYSRWLSSFLPGI
                     TRKGGPWLEPAVVTDRADPKLAHVDGLNLSRAWMLEGIAHGLAPGDPRVEILQSSAAK
                     HREAALPAVTGEHYEGGHWLGTFAVYLTSGAGIRD"
     gene            15735..16199
                     /locus_tag="VGE92_11590"
     CDS             15735..16199
                     /locus_tag="VGE92_11590"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY1314515.1"
                     /translation="MQSLDDKLQWLIDRASISDLLFSFARALDTKDYAAYANNYADGG
                     YVELPDPKAPGSTFVLRKERMLELVPKTLGRYSATHHISANHQITLDGDAASSRSYLQ
                     AVHVSGQPTDHWSAGGWYDCDYIRTPSGWKFTRVRLTPVWVAGQVKDMNPGA"
     gene            16820..16996
                     /locus_tag="VGE92_11595"
     CDS             16820..16996
                     /locus_tag="VGE92_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314516.1"
                     /translation="MIRASFPRGQTLASTQVLDKLETITPGWRQWAERVQTGDLQSID
                     LAPVKSRHSGALTH"
     gene            17035..17340
                     /locus_tag="VGE92_11600"
     CDS             17035..17340
                     /locus_tag="VGE92_11600"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY1314517.1"
                     /translation="MGKKDRGVRMSLQTLKVLEAFLENPTDQLSGADVMQRCQLASGT
                     LYPILLRLESAGWFVSRWESIDPSSAGRPRRRLYRLTPTGLKRASEVFAGFSRGVLA"
     gene            17372..>17711
                     /locus_tag="VGE92_11605"
     CDS             17372..>17711
                     /locus_tag="VGE92_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314518.1"
                     /translation="MTSERTAGWVDGIAQRLIHRAARRAPDPLSERLAEEWLADFAQQ
                     SGLIQRLRFAIGCCWAMTVIAREHPAVAIPATSSATGHGNLIRSPMEDFPFFTGRTIT
                     FVLVASLHVAV"
BASE COUNT         3244 a         5425 c         5670 g         3372 t
ORIGIN      
        1 gtgcgagctc gggctcgact cccacgattt ttatgcgccc cgcataccag gcggaaattc
       61 cggcaatcaa gccgccgccg ccgaccgcga ccaacaacgt ggtgatgccg cctgcctgtt
      121 cctcgagttc caaaccgacg gtgccctggc cccgcaaggt ctcttcttga tcgaaggcgt
      181 gaatgcgcag agctcccgtc tgcgcggccc aggcttcgct cgcggccagc gcatcggcgt
      241 aacgctcccc ggacacgacc aaatcggcac cgcagtcggc tatctgctta agcttcgtcg
      301 acgcggccac cgtgggtacg aatactttgg ccggcacctt gcatttcttg gccgcatacg
      361 ccaccgccac tccgtggttg ccgcccgacg ccgccaccac gccgaccttg gggatgttgc
      421 gcgtgagcag atgggtgaag gcaccgcgta ccttgaacga gcccgcatgc tgcaaggatt
      481 cgagtttcaa ggtcagaggc tcggcatcga tgccgaaatc cgccgcatcg accaccaggg
      541 tcggcgtgcg ccgaatgtgc ggccggatga gggcgtaggt cgcgaggatc tgttgcgccg
      601 agatcatgct gctgttagcg ctcaccttga cggttgccgc cagtgtagcg ggtaacctca
      661 cgcggcagca gccagagcgc gagcgccgag cggtgcgcgg gatcgtggta gaccgtgttg
      721 tccacggcaa tctgccggcg attctcaccg agcgcttcac cggtgttggg gttgacgtcg
      781 aaccgcggga aattgctgct ggagatatcg agcctgatgc ggtgaccttt tttgaatacg
      841 ttcgcggtgt cgaaggggcg gatgagcatc gagtagacct gtcccggcgc gagcagccgc
      901 cgcgtgggcg aggcgtcccg gtagctcgcc cgcaaaatgc cgtcggtgag attcatatcg
      961 aagccctgcg ggaaactctc gctcggcgga tagacatcga tgagtttcgc ggtgaaatcg
     1021 gtgtcgacgc cgctcgaggc ggcgaacagc gtaatctcca cgggtccggc gacggcgaca
     1081 tcgcttggca acggcgcggt ttcgaacacc aagatgtcgc tgcgtgcgcg cagcggcaag
     1141 aacggcgcgc gtgaacccgg catgtcgggc cgttcgcgct gatcgtaggc gccgtccgcg
     1201 aggcgcttcg aaacgccgcc gccaatcgtc ggcaccgggt gcccgggatc gaagtgatag
     1261 gtcgtcgagg caggctccgt cgggagcgcg gcctcgccgc tcagagatcc atcgccatgc
     1321 agatagaagg cggtgcgctg cgcggcggcc ggcggccatt gtttggcctc gcgccactcg
     1381 ccgccgtgca cgagccggcc gcgctcgtcc ttgtgaccgt cgccggtgcc catcagaaag
     1441 tactgtaccg gcgcgcgctc cacggccgag gtcttgcggc ctttcaggta ataatcgaac
     1501 cagcgcagat gaaacgccgt gtcgaaatcg ctgatcgcag ccgcgctgcc gaacgcaaca
     1561 tcgccggcgt agtcggccgt gttgccatga tgcgtccacg gcccgatgag cagctgaatg
     1621 ggcgacttct tgatcgcgct gagcgagcgg aaattctcga tcgtgccgcg caaataaata
     1681 tcgtaccaac cgccgacatg cagcatgggg atatcggcgg tttgtgcgta gtacttctgc
     1741 cattgcatgc caagcgtttg ccaatgcgcg tcgtaatcgg attgggtggc ttcgtcgaga
     1801 tagtagcctt catacgtcgg tgcgatggcg agcggactca agcccttgcg cagcggcaga
     1861 accgaatacc aatccgtgac tttctcccgg ctgagcagcg cttgggtaat cgggtccttg
     1921 gcatcttcga gcgcctgcga ccatgcccaa gtgagctggc gccccatttc gaaggcgccg
     1981 tcatagcgga ccgagtgatc ccaggcgttc gacatcccgc ccatgttgat gaccagcgtg
     2041 ttcaaatgcg gtgggttgag tttcgcggca tcggcctgcg tgtgagccgc ataggacgta
     2101 ccgtacatgc cgactttacc gttgctgtag ggctgccctg ccgcccattc gatggtgtcg
     2161 tagccgtcgt acgcatcgta atcataatac ttgacgaaca cgccctgcga gcgatgccgg
     2221 ccacgcgtgt cctgcaccat caccacgtag ccgtgctcgg ccagcgtctc ggcgatcgcg
     2281 accgtcgctg cctcggattt atcataaggc gttcgatgca acaacaccgg caagcgttgg
     2341 gtgacggctg cgccgccgtt ggcgggtcgg taaatgtccg tcgccagcga gatgccgtcg
     2401 cgcgcgcgca ccatgacgtt gagttgtcgc gagactgcgg agtacggggc ctgcgccgac
     2461 gatcccgtgt cggcctgcgc gcttaagctc aacagtgctg cgatgagcag caccggcggt
     2521 cgagcccatc ggcaaacccg cattacgggc cgttcgaagc ggttaatggg tgctgagcgg
     2581 gtattttgac aatgagatac gtgaacggcg cgttggcagc gaccgtcacc aggtgcgggg
     2641 atcccgccgg tatcagcagc agatcgccgg cggccacggc gtactcgcgt ccgccgctct
     2701 gttcgccgcc gcgccattca ttggcctcgg tctggcgatt acctgtgacg cgtccaccca
     2761 cggtgaccgt cgcgcggcca ctttcgacca acaggatatc gtcgagagcc gcgtgcacct
     2821 cgacttcgcc tgttttatcg cgtcgcgcaa tcagtatcgt cgattcgggc ctgctcgaaa
     2881 ccgctttgaa gtcgaagccg tcgactgggt gagccacctg ggctgcaagc tgcgccgcgg
     2941 agaagtactg gccctcgtcc actttcgctc tcttgaggtc gccgcgggca acgaggctca
     3001 ccatcaggag gagaaccgat aaacccatcg cgcgcagggt tgcagtaggc atggtcgttc
     3061 ccgttgttgc cggaggagca atcttgggag attagtccac aatcgaatga gacgtgcaac
     3121 cgtcgcccag ccgcacacgt ggcgactcgc ggtaacccta gaagggtaaa gatctgtgat
     3181 tttcatgaaa agttccgtga tttggggcaa cgagtttgtg cactccgcgt taactcatgc
     3241 tgcaatccgc ttcgcagtgg aggagattca ttatggcgat gaacggatcg aagactcgcg
     3301 gggcgctcat gccctcgctg gtctcaatgc tggtggcagc ggtcgtgggt ttatgcctcg
     3361 cgccggcggc ccgcgcaggc ttgggagaga ctgcggcctc ggtgcagcgc gatcacacgg
     3421 cgctgcgcgg gacctcgttg accgtgacgc cgatggcgtc ttacgacctc catgagatta
     3481 cctcccccga cagccgggtg cgcgaatacg tctcgcacgc cggcacggtg ttcgcggtga
     3541 cctggtccgg gcgcgcacgg ccggatctgt cggtcgtatt gggagcgcgc tacgccgagt
     3601 actcgaaagc ggcggcgacg caccgcggaa gtcacaaggt atttgcgttg tcgtcggacg
     3661 atctcgtcat gcacgtcacg aagctgcctc gcgggttcga gggcgaggcg catgcgccaa
     3721 cattgttccc tgcgggcgtg agcccgcaag atattcgctg accgcaagga tcatccagtg
     3781 acaaactcaa ttcaccgagc cgcttggctc agctgcgcgt tatttctggc cgcctgtagc
     3841 ggcggatcca atgcccccgg ggtgacgctc acttcgattg ccgcgacgcc gctgtccatt
     3901 cctttgtcag tcggcggcac gcaagcgctg acggtaacgg gcacttacag caatgcgacg
     3961 acggcgaccc tcgcgtcggg cctcacgttc gcttccgaca acgccgcggt tgccaccgtg
     4021 agcaacgccg gcctggtgac tgcggtggcg gacggaaccg cgcatatcac cgtcaccgac
     4081 tccgccgcgt ccttgagcgc gacgccggtc acggtcagcg tgactgcggc cgcttcgacg
     4141 gccaatacgc tggcggtcgt cgtcgactcg gggccggccg cactcctagc ggaaaacccg
     4201 cccgaggtcg acgccaacgt tctgttcgcc accgtcacga tttgcacgcc gggcagcctc
     4261 accgcgtgcc agtcgatcga ccatgtgcag gtcgataccg gttccatcgg cctcggcatc
     4321 atgagcgggg ttttgaacgg cgcggccgtg ccgcagccga tccaggtcag cggtggtccc
     4381 ttgcgtgaat gtatcgcgta tgcggacggc tacgtgtggg gctcgatggc gaccgtggat
     4441 gtgcagatcg gcggacgcac catttcatcg ctgccggtca acatcatggg cgacccggcg
     4501 gcgggcaccg ctcctgcaag ttgtacgtcg ggatacacga gttcgcaaaa cgaatcatcg
     4561 gtccttgcat tcggtgccaa cggcgtgctc gggataggcg ttttcctgca ggattgcggc
     4621 atcgcatgca ccacggaacc ggctcccacc caagcctatt atgcgtgccc gacctccggg
     4681 tgcgtcgccg cggccgccgc actgaatcag cagatgaata atcccgtggg cgcgctgtcg
     4741 gcggacaaca acggcgtcgt catcgtgctg ccggcggtca cgtccactgg ccaggtctcg
     4801 gtgagcggca ctttgtatta cggaatcggc actcaggcca ataacgccct cggcagcagt
     4861 acacagctgc tcacggtgct cgattccggc acgacggcgg gcacgctgac aacgaccttc
     4921 aacggctcga cgctcgctgg cagcgtcatc gacgccgggt cgagtggtta tttcttcaac
     4981 gactcgtccc tcccggcctg ctccgaccag cccgcgtatt attgtcccgt cagcggcagc
     5041 gcggcggcca cagtgtcctt caacgccacg atttccgggg aaaacggcat cacctcgccg
     5101 caggtgttca cgatcgacaa cgccgattcg ctgttcagca gttcgcaaac cctcacggcg
     5161 tttccgacgc tgggcggccc gaatggtgcg ctcaatggga ttatccccgg cttcgattgg
     5221 ggactgcctt ttttctacaa ccggtcggtc tacgtgctct tcgagaccta tgcggtgggc
     5281 ggcacgaccg gacccgcgat cggtttttga ttcgcgctcg accgccgtcg gcggcgcggc
     5341 gttgggtttg aattttgctt ggcaatactt tgcgggtctt cacgcagcgc cgggtcaacg
     5401 agatgccgca aagacgttga ttatactgtc aggaaaattt ttcctgacgg cggcggcgga
     5461 aaatcacagc gcggcctttc tgttatgaac gaggaacatt gccatgaaaa gtttacgacc
     5521 cttaatgcga cccatcagtt gtgctctgat tctcgcggcg tcggttgctg ccggcaccta
     5581 cgccaacgcc caggcgacga ccggtgccgc aactgcagca ccgagcgggc atcattggcg
     5641 ccaccgtcaa ttcggcccga tgcacgtgct gcacaagctc aatttgactg cggaacaaga
     5701 gacgcagatt aagtcgatta tcgaacaggc gcgcgcgcaa gcgaaaacgg agatggagag
     5761 cgagcacgcg aatcacgaat cgctggcgtc gacggcgccc accgatgcca actatccagc
     5821 cctgctcgcg gcggccaaga ccaatgccgc aaaccgcgtc cagcggatga gcgaggtgtg
     5881 ggagcaggcg tatgcggtat tgactccggc gcaaaaagcg cagatccccg caatcctcgc
     5941 tgccgagaaa cagcaaatgg aggcccgcaa ggcggcctgg caggcgagcc acccgcagtc
     6001 ctgatgcgga tccgacgcac ggcgctcggt atcatcactc aggtcgatat tgagagtgat
     6061 accgggcgcc tgcgccgatc tcgcgcgctc ttgacgtttc tttgcgctcc gctgggttca
     6121 gggcgcaaca cctaagccga taaacgaggt tgaggggaag tccattgggc gcacccgacc
     6181 cgcattcggc gcggcgcgtc cgtcatcagg agtatccaca tgagcgtatc gcccatcagt
     6241 tcagccggtc gcgggctgaa ctcactgagc tcgagccaga agctgcaaca gtatcttcag
     6301 gcattgcagc agtcgcttgc caccggcaac ctcagtggcg cccaaagcgc attctctgcg
     6361 ctgcagtcgc aggtccggtc ggctgccggc gcatcgggcg catcgggcgc accaggcgcg
     6421 tcaggtgcat cgggtgcatc cgcgaccagt gctgctgcgt cgacgaatgg cgcgcagtcc
     6481 gcgctttcca ccgcgtggga aacactctcg acggcgctct cgagcaataa cctgtccgct
     6541 gcgcagaccg catttgcggc gctgcaggcc gcggcgaatg ccggccaaca gtcggcctcg
     6601 agtcccccgc ttgctagcgc gatcgcggca tcaactcacg cgcaccacgg tcgtcatcat
     6661 cacggcggtg gcgggtctcc atccagcacc gaggctgcct ccgcatcgag cgcggctacg
     6721 tccgcatcga gcaccgacgc gtcgacaaca accccaagct cgccgggcgc tttgctcaac
     6781 gtcacggcct gagcccgccc cgaggcctgt gagccgacca cctagctccg cttgacaggt
     6841 gaccaaatgg tcacgtatgg tcgcgaccat ggatgaggtg ttcaaagcgc tcgccgatgc
     6901 gactcgccgt aagctgctcg acaagctgtt cgagaaagac ggtcagacgc ttcaagcgct
     6961 ggaaaatcgg ctgccgatga cccgcttcgg ggtcatgaag catctgaagc tgctcgaggc
     7021 ggcgggcttg atcgtgaccc gccgacaggg gcgcgagaag ctgcactttc taaacgcggt
     7081 tccgatccgc ctcatccacg accgttgggt ggacaagttc accgcgcctt gggtcgctgg
     7141 cttgagcgac cttaaacacc gcttggaggg tcaaaccatg caaaaggtat tcgaaatcta
     7201 tatcaagacg acgcccgcgc gtctgtggaa agcgctcacc gataccgaga tgcggcgcaa
     7261 gtacacgttt ggggcggagg taacctcgga gtggacgcca gcttcgcgct accacggcgt
     7321 gggtcatgga gcgcccatct tcgaaggcga aaatttagag gtcgatccac cgcggcggtt
     7381 ggtgcagagc tttcgcgcgc tgtggggcga ggatgtgaaa agcgagggca cctcgcgtgt
     7441 aacgtgggag atcgaaccgg tcggcgattc gtgtttgctg aaagtgacgc acgatcagtt
     7501 gcgcgaggac gccaacaccc agttgtacgg cggttggccg atggttctct ccggaatcaa
     7561 gacgttgctc gagacgggcg agatgctgac gacgccgggg tcgctgcgct gggtgcaagg
     7621 cgcgcagggg caatcatcaa ttgcttgagg cgcgccgatc gggcgaggta tctttgcgag
     7681 cattgcacga cgccggcgga ccatcaacat gtatgagctc tacatagcca acaagaatta
     7741 ttcctcctgg tcgttgcgcc cctgggtgct gatgcgcgaa ctggggattg cgtttcgcga
     7801 gcatctgctg ccgtttggcc aggaatcgct gtggagcgcc tttcgcaaga tttcgccggc
     7861 gggcaaggtg ccttgcctga tcgatggcga gacggtggtg tgggattcgt tggccatcgt
     7921 cgagtatctc gccgagagcc accccggcgt gtggccgggc accgcggccg cacgggcgtg
     7981 ggcgcgcagc gcggcggcgg agatgcactc cggattcggc gagttgcgca gccgctgttc
     8041 catgagttgc ggtcagcgga tcaagctcca tgaatttccg ccggccttgg aacgcgacgt
     8101 cgcgcgcatc gacacattgt ggggcgacgg cctgcagcgc ttcggcgggc cttttctcgc
     8161 cggtgcggcc ttcaccgcgg tcgatgcctt ctacgcgccg gtggctttta gagtccaggg
     8221 ctacgggctg agcttgggga gcgcggcggc cgcgtatgtc aatcgcctgt tggagacgcg
     8281 ggcgatgcgc gagtggtacg ccgatgcgct caaagagacg attcgcgacc agccgcatga
     8341 agaggacgtc gcgcggatgg gacgggtgtt ggaggactcg cgcgcgaagt gaaggccgtg
     8401 ggatttcagc tgctcgccgc ggatgaaccg ccgccgttcc ttgaattggg gcgccgcgga
     8461 cagtccaatt tcgtgatcat cgtcgatcat gccggcagcc gcattccgcg gcgcctgaat
     8521 aatctcgggc tgccggcctc cgaattacag cgccacatcg cctgggacat tggcgcgctc
     8581 agtgtggcgc ggcaaatggc ggcggcgctc gatgcgccgc tgctcgcgca aaactactcg
     8641 cggctggtga tcgactgcaa tcgcgacccg aaggtcgcga gctcgattcc gcaatcgagc
     8701 gaagcgatcg agatcccggg caaccgaaat ttgagcgagc cggaagttct cgcgcgccgc
     8761 gccgagatat tcgagcccta tcacgaacgc gtgcgggcgc tgctcgacga aagagcggcg
     8821 gcgaaccaga ccacgatact ggtggcgcag cacacgatga ccgatgtcta ccacggcgag
     8881 cgccgcgcga tgcacgccgc ggttctctac aaccgcgacc gccgctttgc cggcctcgtg
     8941 ctcgactcgc tgcgccgtga cggcgcgctc aacatcgcgg acaacgaacc ctacttcgtc
     9001 agcgacgaga ccgactacag catcccgcgg catggcgagg cgcgcgctct gccgcatatc
     9061 gaaatcgaaa ttcgccagga cctcgtgagc gaagccgccg gacagacgca atgggcgcag
     9121 cgaatcaccg ccgccttgcg tgaggcggag cgggtgtttc tcgcagcgcc ggcgtaatga
     9181 caccctcgcc agcctccagg agcgcaggcc gcgatcgggt ttgccggctg cgccgcgaac
     9241 tgatatagga acacgaacgg cgaggagtgc gcgcatggca aatattgtta acaagccggc
     9301 aacggcccgt gtgggctgaa ctcgaaccga agtaagcatg tacgccgccg ttcttgccct
     9361 gtttagcttg gtcggcgcgt gggtggcatg gcggcgcaat ccttcgtata cggcccgctc
     9421 caccctgcga acgttgctgg tcacggcgct ggcgatcgcg gtgttgatcg ggatgatcgt
     9481 ggtcgcggtc aatttttcga tgaatcgctc cgccccggtc gcgtacgcca ccatggcgac
     9541 ggtcgtcgtc gtcggcagcc tgtccatgat tttcatcctg caggccgcct cgacccccaa
     9601 ggacgccaag ctcatcacgg ccctgccggc atcggccaaa attctgcacg tccaccggca
     9661 gaaggtttat cactggtcca aggtgttcgc cgtcattatt gcggtctgcg ggctgctggc
     9721 attgttgatc cccggagatg ccaaatatat tccgctcgcg ttcggcggca tggcgttgct
     9781 gctcgcatcc atcctgttgc cggtgctgta cgcgacaacc cgcaccgcgg atcgatcgct
     9841 gacggcgctc gaactcaacc cctgggtcca ttggcgatat acgccggcgc aatggcagca
     9901 gtggaccgaa gtccaagtcg ggcgcatgaa ggcaacacca cccacgttca tcttgaagcg
     9961 cgactggcac aaacttgcct gggcattcgg cgccatcgcg gccggcgtcg gcattttctc
    10021 gccgggcacg tggctcgaga aaggcctgta tgtgatctgc gtttgcggcg cgatcacggc
    10081 gctggtcatg ctgagcgccc gctcacaaca tcgaatgccg gagatgaacg cggccaagct
    10141 cgccaaaatc gagccggaag tttacttcgg acacgacgga ttatattgcg acggcggtta
    10201 tctcacgtgg ctgggtttga gcgtctatct cgtggcggcg tccgtcgaca cgcgagcgcc
    10261 gcgaagcctc gcgttcaatt tcgagaaggt gatccccaat ccttacggtg cgaccgtgcc
    10321 gctgcagatc caccaaagcg tgctgatacc cgagggggcc gacgccgaca tcgcacggct
    10381 gcagcgtgaa ctcagcggcc gttgtcccaa ggcgcacatc gcgctcgcgt gagtcatcgc
    10441 gtgttaagaa cccgcactgc gtcgcaggcg gccggaaaaa taataagcgg ccgtgagcgc
    10501 ggccacggcc ggcgacaaca gcggcagcac ccattgcccg acggcccaca ccttggagtc
    10561 gccgggggcg aagatccaca gagggatgag cacgagcacg ccgatgagcg cgatgcgcgt
    10621 gatttggaac catagcttgt ggaaataatg tgcggtcgca gcccgctccg gtagagcggc
    10681 gtccgacacc ttgaaccagc gcccggccag tgcggcagcc ggcaccatgg cggcgggaaa
    10741 accgaccgag agactgatga tcagatcgag cccgagctgc aaaaagaaaa gcggggcggc
    10801 cgcctgtgcc ggcagggagt tcattccggc aatcaccacg gcgctgagaa tcagcatgaa
    10861 aaaaatcacc gtcaaggcca gccacacgaa gtattcgatg cccgggaccg gcctggcgat
    10921 ggactgcagc tgggtcttct cctctggggt gtagccgaag ccgggccagt tggttccctg
    10981 atcgtggatc agggtcatgt tgaacgccca atagcgcacg agcagggcga gccagcgctc
    11041 caaacggggt ctcggggcgg gattatccga catgcgcccg agtgtaaggc cgaattgcgc
    11101 aagaccaaag gtccgtcacg ccttcggccg cccgatggga taatctagcc atattatgtg
    11161 tgatcgttat gtcattccgg accaggtggc ggctgaacgg gagttcctac cggcccaagt
    11221 ttggtggaaa ttcgagccgc attttaacgt cgcggcgcgt cgctacgttc ccgcaatccg
    11281 agtgcacgat gcccagacgg aaggcatgat gatgcgctgg gggttgatac cggcggagac
    11341 caaaggcaag ccgtcgggta gttcgcgggc ctgtgccgac atcgaacgtg tcgaacgctc
    11401 caagacctac aagatgccct ggcttaacgg tcagcgctgc attctgccgg cggcgggatt
    11461 tttcgtttgg aagctcacgc ccgcgaatta ccgccagccc tactacgtgc gggtgaataa
    11521 ccgcagcgtg ttcgggtttg ccgcggtttg ggatcgttgg agcggcgaag atgaggacgt
    11581 catcgagagc tgctcgatca ttcgcgtgcc ggccaactcg ctgttggcgg acgtggccga
    11641 ctcggatcgg atgcctgccg tcttgaagcg ccgcgactac gacacgtggc tgcgcggcac
    11701 gccggttgag gcgcgggcgg cgctgcagtc ctataacccg gagtggatga ctgcgtacgc
    11761 cgtcagcccg cgcatcaact cgcaggaacc ggacgatgcg gggctgattc agccgatcga
    11821 cgcatgaggt ggattggcct gctgctcatc tgcgctgcgg cgtgcggagc ggccgcggcg
    11881 caagacgtgg cgctcatcac cgccgccggc cagcggcaga tgctcgcgag tccgggcacg
    11941 cccggcgtcg gtgcgcaaaa gcccgacgtg gtcatcgtcg aatacttcga ctacaactgc
    12001 ccctactgca agaagctcgt gccgacgctc caggcgctgc tcgcgcagga tccaaaaatc
    12061 ggcatcgtgt acaaggattg gccgatccta ggaccggtct cggcgtatgc ggcggccgcg
    12121 gcgctcgccg cgggttggca gggcaagtat ctcgccgcgc acgatgcctt gatcgtaggt
    12181 cctcgcttgg ccgcgaacac gcaggtcgat gcgctgttac agcgggcggg ggtggatctg
    12241 ggcgcgttga aaaaagatcg cactcgtcac gccaaagaaa ttgacgcgct gctcgcgcgc
    12301 aacgaccaag aagcgcacgc gctcagtctg gacgggacgc ccggcatcgt ggtcggtcgg
    12361 ctgctggtgc ccgggatcgc cggtgtggac gacctgaaac aattgatcgc ccggtcgcgc
    12421 caatcgaaat agcgagggcg tttacggcac cacgtgtgaa gcgccgacat ccttccattc
    12481 gtagcgcgga acctgtatcc actggccgct cttcagttcc ggtactttgt gcaccttcat
    12541 ctgatcgacg accatgctgc cgtccttgtc gctgacccaa aaatcgatgt cgtaaatctg
    12601 atccttggtg ccaaccactc gaaagtcggt gcaggcgaag taatggccat cattgtcgag
    12661 ctgccgcacc ggttggtggg tgtcgacgaa ctccagttcc aactccttgc cggtcttgtc
    12721 atccttgagc ttgaaaaccc cattgttctt caagctgtct tgcacggcgc gctgttcgac
    12781 agccgacatg acttcccagc ctcgcttggt ggccaagtgc ccggggtgtt cggaggccgg
    12841 aatccaccac cacggaatgg gttgtcgggc catggtgacc cacttgccgt ccgacttcag
    12901 cggctcctta taaatacgcg tctcttggat ttgcagcttg ccggccacgg gcgtcaccca
    12961 gaaatcgacc aggtattttt tctgtgcatc ctgcgaatcg tgaaatttca cgtccggaaa
    13021 aaagccgtag ccatcgatgg tgcgtgtgaa atcgacgtcg tcgtaattca atttgaggtg
    13081 atcgccattg agcggatcgt cgaaggggaa tagcccattg tttgccttga gcttgtcggc
    13141 gatgaacccc ttgaccaggg tttccgcatc gctgccggtg aagtcctgca tggccttctc
    13201 ggtatcctcg acgctgcccg cggccatgaa ggcgcgggcc cgctgcagat tttccgccgg
    13261 acttgccaag aaggtcttct tggccgcgtc attgctgaag caataagtct tgccgtcttt
    13321 gtcggtccat gtgattttgc agtcggtggc ctgatgcttg ccctcggcga gcgcttcggc
    13381 gcactggccg ccgaactcgg tctgcgctgc cgccggtgaa tcggcgcagt gcccggcgaa
    13441 cgggatgagc agcagcgcgg cggcggcact caacgaaagc ccacgcaagt cgaactttgc
    13501 catagcacgt ttcccccaaa gttcagctat gtataagccg attggccatg gaagtcgatg
    13561 ccctggggga ggcgcggcac gccgggcact ccacggggac actcgagtgc ggcccgatcc
    13621 caatcggcgc tgacggcggc cgcttcgtac gtgctttgca ggaacgcgag caacgccgcg
    13681 tccggatccg ccgcggtgcg caccgcctcg tagggcagca agaactcgcc gagttgtgcg
    13741 ctgaaaaaag cgccggcggg ctgcaccgca atctcaccga agcgggccga gctcggatag
    13801 gcgtacgaat agaaggcggg gtagtcgatg ccgttgcctc ctggccagaa cccggcgctg
    13861 ctctcctcgt gagaataggc ttcctgcatc accgccgcag tcactcccgg cgccgtgcct
    13921 ggataggtgg gggcgcggcg gccggagaag cgcgtgacgg tgatgtcgaa gctcccccag
    13981 aagaagtgca ccgggctgca cttgccgagg aagccggtac gaaaacgctt tagcacacgg
    14041 tctgtctgca gcagaacctg ccagaagcgg tgcgcgtaat tcggatcgta cgccgcgtgg
    14101 gtccggtcct ggctgaacgg aatgcaattg gcgatctcgc aggggtgttc gttgatggcc
    14161 acgggaacgc cgagatcggc gagggctgtc atgaccaccg cataaaaatc cgcgacgctt
    14221 tgcggccgca acggcagtcg ccgcgtattg ccatcgctca gggtgatgtc gagcgtatgc
    14281 tcgagaaaat tgaattcgat ctcgaagctg cgcgcgccgt gcgggatggg cgacgtggtg
    14341 aggcccttgg tggacacgta cagcgctaca tgccagctat gattgagcca gggcgtttgc
    14401 atgaggcgca ccttgccgac gatctgcgtc cacaactgca gggtggcgcg cgtgtcagcc
    14461 cacgcagaga cggggagctc gggccattgc gggttttttg gattgaccat ggttctccac
    14521 gggggttcgt gacccggcga ttctggttgc agcgcgacgg cattgtctta acaatggaga
    14581 ttaccgcgtc ctcgggagtc ctgtcgtgcg tcgagcccat tacctcaccg ggattctgct
    14641 ggcgatgacc ggtgccggcg ccggcgcggc caccgtcacg agcgagcagg ctctggcgat
    14701 ggacccggcg gcggccgcgc gcttcgccgg tcttgccctc aagtgcttgc acgccgagta
    14761 tccgaaccac atcagtcata cgctggacag cgacgccgac gcgcttgcgc cgcatgtgct
    14821 ctaccccgcg ttttacggct gcttcgactg gcactccgat gtacatgggc actggcttct
    14881 ggtccgattg ctgcgcgtac ttccagaggc accgtttgcg aagcaggcac ggacggagct
    14941 tgctcgcagc ctcacgccag agaatatagg cggggaagtc gcgtatctta agcgcgccgg
    15001 ccgcgcctcg ttcgaacggc cgtatggtct cgcttggctg ttgaaattgt cggcggaact
    15061 gcggctttgg aatgatgcgc agggacgcga gtgggcgggc gcgttgcggc cgctcgagac
    15121 cgaggccgcc gcgcgactca agagttgggt gccgaagctg tattatccga ttcgaatcgg
    15181 cgagcacgat cagaccgcgt tctcgttcgg cttgatctgg gattgggccg cagtcgccgg
    15241 cgatgccggg atgcgtgatg tgctgaagga tgcggcgcag cgcttttacc tcaaggaccg
    15301 ccactgtccc ctcgactacg aaccgtcggg cgaggatttt ctgtcgccct gtcttgccga
    15361 ggcggatttc atgcgcaggg tgctggatcg agccgagtac agccgctggc tgtcgagctt
    15421 tttgccggga ataacgcgaa aaggcggacc gtggctcgag ccggccgtgg tcaccgatcg
    15481 cgccgacccg aagctcgcgc acgtcgacgg actcaatttg agccgggcgt ggatgctcga
    15541 gggcatcgcg cacggactgg cgccgggcga tccgcgcgtc gagatactgc agagctccgc
    15601 ggccaagcac cgggaagctg cgctcccggc cgtgaccggc gagcactacg agggcggcca
    15661 ttggctgggg acgttcgcag tgtatttgac cagcggtgcc ggaattcgcg attaacgcag
    15721 gggataacct acgcatgcaa tccttggatg acaagctcca atggttgatc gaccgggcgt
    15781 cgatcagcga tctcttattc agcttcgcgc gggcgctcga caccaaggat tacgccgctt
    15841 atgccaataa ctacgcggac ggcgggtatg tcgagttgcc ggaccccaag gcgcccggca
    15901 gtaccttcgt gctgcgcaag gagcggatgt tggaactggt gccgaaaacc ttggggcgat
    15961 acagcgccac gcatcacatc agcgccaatc atcagatcac gctcgacggc gatgcggcga
    16021 gttcgcgctc ctacctgcag gcggtacacg tcagcgggca accgacggat cattggtcgg
    16081 cgggcggatg gtacgactgc gactacattc gcaccccgtc ggggtggaaa ttcacgcgcg
    16141 ttcgcctgac gccggtgtgg gtcgcggggc aggtcaagga tatgaatccg ggggcttgag
    16201 acatcgtaag aacgtacgct cgatactcga tgatgcgtcc gcgactctgc ttcgcgtttt
    16261 gcttcctgtt ctcgtgtctt cacgcggcac cgcaaagcct cgctgatgca gaggattcga
    16321 ggcgcatcga ccaaatccat tccctcggtc tggcctctct ccctggaaag atgccgacgt
    16381 tttacagtcc aggagcaagc gctcgtgccg agtatcttca aagcttgctc ggcggcgaaa
    16441 tcgcctattt cgccaagctg tttcacatcc actttgcacc gctgaacatg gcggtactcg
    16501 acgccgctca gtggcccctc gttgcaggcc aagacccgta tggcatgccc ttggtaggcg
    16561 gatccaagcc gagcgtcttc gtaatgcccg gtcccggccc ctctcctcag acgggctctg
    16621 gccaatggaa gaacgtggga tcaggtcaag tttgaaggct gcgacggtat cggcactcat
    16681 gagattggtc attccatcat tcatgaacta agaatcgatc cacaaacgag gtggttcaac
    16741 gaatttctgg ccagctacgt cggcacgaaa ttcgagagtc atggagattt accggactca
    16801 tggtgtcgaa tttttgcgca tgattcgtgc gagttttccg aggggtcaga cgctggctag
    16861 cacgcaggtg ctggataagc tcgagacgat cacgcctggt tggagacaat gggcggagcg
    16921 ggtgcagacc ggcgatctgc agtcgatcga cctcgcgccc gtaaaatcgc ggcacagcgg
    16981 tgctttgacg cattgaactt gcgttgccgc gagaccctat gtagacttcc tactatgggt
    17041 aagaaagatc gcggcgtccg gatgtctttg cagacattga aggtactcga ggcattcctg
    17101 gaaaacccca cggatcagct gtcgggcgcc gacgtgatgc agcgttgcca actcgcctcc
    17161 ggcacgttgt atccgattct gctgcggctc gagtccgcgg gatggttcgt gagccgatgg
    17221 gaatccatcg acccctcgag tgccggccgt ccgcgccggc gcttgtatcg attgacgccg
    17281 acggggctca agcgggcgag tgaagtattc gcaggtttca gtcgcggtgt tcttgcataa
    17341 gcgggcatag ccatcggtgc caggagggcc tatgacttcc gagcgaacgg cgggttgggt
    17401 ggatgggatt gcgcaacgat tgattcatcg tgccgcgcgt cgcgctccgg atcccttatc
    17461 ggagcgactc gcggaggagt ggttggccga ctttgcgcaa caatccggtc tcattcagcg
    17521 tctacgcttt gcgattggat gctgttgggc gatgactgtc atcgcgcgcg agcatcccgc
    17581 ggtggcaatt cctgcgacca gctcggccac ggggcatgga aatctcattc gctcgccgat
    17641 ggaggatttt ccattcttca cgggccgtac cattacgttc gtgttggtcg cctcgctgca
    17701 cgtggccgtg c
//
LOCUS       DASWZT010000409         5397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_13112, whole genome shotgun sequence.
ACCESSION   DASWZT010000409 DASWZT010000000
VERSION     DASWZT010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13112"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1358)
                     /gene="crtI"
                     /locus_tag="VGE92_11610"
     CDS             complement(<1..1358)
                     /gene="crtI"
                     /locus_tag="VGE92_11610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HEY1314519.1"
                     /translation="MRSPRIAVIGAGVAGLVAAADLARIGFEVLLFERAGTPGGKMRR
                     VSIDGRSMDAGPTVFTLRRLFDELFDDAGDSFERRVNLTSASVLARHAWSSQERLDLF
                     TDIEQSAQAIGEFAGRRDAQGFRRFAAQARRIYETLDDPFMRASRPTPLALMRRIGLA
                     RTGDLLNIQPFISLWRSLSRYFRDPRLRQLFARYATYCGSSPFASPATLMLVAHVEQA
                     GVWYIGGGMHELAVQLAALAARQGATLRYACEIARIQLHRGRVDAVHTVHGERIPVDA
                     VVCNADNNALAGGLFGAEVCGGVRATPAAARSLSALTWNLLARTEGFELAHHSVFFGA
                     DYRAEFADIFHRHRLPSKPTVYICAQDRQDDAARAPQSRERLLCLVNAPPIGDSHLFD
                     TAEIDACKARVSETLANCGLKLHFQGDGVTTTPADFNRMFPGTGGALYGPASHGWMAS
                     FKR"
     gene            1468..2328
                     /locus_tag="VGE92_11615"
     CDS             1468..2328
                     /locus_tag="VGE92_11615"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY1314520.1"
                     /translation="MTRIERSLGQAFGLAKGSGCPPSLNAAMRYAVFPGGARIRPRLC
                     LAVARACGDDKPEVAASMAAAVELMHCASLVHDDLPCFDDAAIRRGRASVHKAFGERI
                     AVLTGDALIVLAFEALARSAQAAPARLAPLIGILARAIGAPNGIIAGQAWECEPQISL
                     AHYQRAKTGALFAAATAAGACAAGCQAQAWHLLGERIGEAYQIADDIRDVVSNAHELG
                     KPTGRDQALGRPNAVHKLGMGSAVQRLEELVSAALDTIPDCPGANDLRAHILAEASRL
                     LPRELARPAA"
     gene            2340..3470
                     /locus_tag="VGE92_11620"
     CDS             2340..3470
                     /locus_tag="VGE92_11620"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY1314521.1"
                     /translation="MPWTDRYLSVRDRWLSSPRFQRWAAAFPLTRPTARKRARALFDV
                     CAGFVYSQILFACVRLKLFDLLATGPLTAPSIAAKLNLPPDSTERLLEAAVSLRLVEK
                     RTAGRFGLGALGAAMVGNPAVGAMVEHHSLLYADLHDPVRLLRGEQSQTALARYWAYA
                     HSDKPAGLPAAQIADYTALMGNSLPLVADEILDAYPLHKHRSLLDVGGGDGSFLKAAA
                     ARSEHLKLMLFDLPPVAARAAQRFTTLGLDSRARAVGGDFLKDPLPHGADVISLIRVI
                     HDHSDAAAAVVLAAAQRALPPNGVLLLAEPMMGTPGAEPVGDAYFGFYLLAMGSGRPR
                     TPQALQAMLCAAGFASAQLVRTRQPLQSRLIVARKAADGKVS"
     gene            3501..4490
                     /gene="bchC"
                     /locus_tag="VGE92_11625"
     CDS             3501..4490
                     /gene="bchC"
                     /locus_tag="VGE92_11625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorophyll synthesis pathway protein BchC"
                     /protein_id="HEY1314522.1"
                     /translation="MTLRTMTRTAETPYPSLNTLAVVLEKPEQLVLSRLQLSAPGDED
                     VVVDMEWSGISTGTERLLWSGRMPAFPGLGYPLVPGYESVGRITHAGAHAARQVGDRV
                     FVPGARCFGEVRGLFGGAAAKVVVPGARVTPISENMGEQGVLLALAATAYHAVTAQNT
                     NLPDLIVGHGVLGRLIARLAMALGAEPPLVWERNPNRIDGSVGYTVLHPDADVCRGYR
                     AIYDVSGDNTLLDTLIARLAPGGEIVLAGFYSQSLSFAFPAAFMREARIRIAAEWRDG
                     DLKAVRQLVDSGRLQLDGLITHRREASAAPAAYRTAFTDPSCLKMILDWRAQS"
     gene            4487..>5397
                     /locus_tag="VGE92_11630"
     CDS             4487..>5397
                     /locus_tag="VGE92_11630"
                     /EC_number="1.3.7.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorophyllide a reductase iron protein subunit
                     X"
                     /protein_id="HEY1314523.1"
                     /translation="MSTAFANSSALRSEQVRLEALRAEAAIEPDAVPSTAPTKQTQVI
                     AIYGKGGIGKSFALANLSYMMAQQGKKVLLIGCDPKSDTTSLLFGGRACPTIIETSAK
                     KKLAGEPVKIGDVCFKRDGVFAMELGGPEVGRGCGGRGIIHGFELLEKLGFHDWGFDY
                     VLLDFLGDVVCGGFGLPIARDMCQKVIVVGSNDLQSLYVANNVCSAVEYFRKLGGNVG
                     VAGLVINKDDGTGEAQAFAAAAGIPILAAIPANDDIRRKSANYDIVGRPGTEWGPLFE
                     ELAANVAEAPPLRPKPLTQDQLLALFK"
BASE COUNT          960 a         1680 c         1773 g          984 t
ORIGIN      
        1 cgtttgaacg atgccatcca tccgtgggag gcagggccgt acagcgcacc gccggtcccc
       61 ggaaacatgc ggttgaaatc cgcgggagtg gtcgtcaccc cgtctccttg gaagtgcagc
      121 ttcaggccgc aattcgccag cgtttccgag actcgggctt tgcatgcgtc aatctccgcg
      181 gtgtcgaaca agtggctgtc tccgatcggc ggcgcattca ccagacacag caaccgttcg
      241 cggctttgcg gcgcgcgcgc cgcgtcgtcc tgacgatcct gcgcgcaaat gtaaacggtg
      301 ggcttcgacg gcaagcggtg ccgatggaaa atgtcggcga attccgcccg gtagtcggcg
      361 ccgaagaata ccgagtgatg cgcgagttcg aatccttcgg tgcgcgccag cagattccaa
      421 gtgagcgccg acagcgaacg cgcggcggcc ggtgtcgcgc gcacgccgcc gcagacttcg
      481 gcgccgaata aaccgccggc gagcgcattg ttgtccgcat tgcaaaccac ggcgtcgacc
      541 gggatgcgtt cgccgtgcac agtgtgcacg gcatcgacgc ggccacggtg cagctgaatt
      601 cgtgcgatct cgcatgcata gcgcagcgtt gcaccctggc gggccgccag ggcggcgagc
      661 tgcacggcaa gctcatgcat ccctccgccg atataccaaa caccggcttg ttcgacgtgc
      721 gccaccaaca tcagggtcgc cggcgaagcg aacggcgagg aaccgcagta cgtcgcatat
      781 cgagcgaaca gttgtcgcag ccgcggatct cgaaagtaac ggctcaacga acgccacaag
      841 ctgatgaagg gttggatatt caacagatcc ccggtgcgtg ccaagccgat tcggcgcatg
      901 agcgccaagg gcgtgggacg cgaggcgcgc atgaaggggt cgtccaaggt ttcataaatg
      961 cgccgcgcct gcgcggcaaa tcggcggaag ccttgcgcgt cgcgcctccc ggcgaactca
     1021 ccgatcgcct gcgccgattg ttcgatatcg gtgaacagat ccaaacgctc ctgcgaactc
     1081 cacgcgtgtc gtgccaagac gctggccgac gtcagattta cacgccgttc gaagctgtcg
     1141 ccggcgtcgt cgaagagctc gtcgaaaagg cggcgcaggg taaataccgt gggtcccgca
     1201 tccatggaac ggccgtcgat gctgacgcgg cgcatttttc cgccgggcgt ccccgcccgc
     1261 tcgaacagca ggacctcgaa tccaatccgg gcaaggtcgg ccgcggccac caacccggcg
     1321 acgcccgcgc cgattaccgc gatgcgtggg ctgcgcattc cttacccgcc gattgtcgtt
     1381 gaccagggtt tcatcaatgt ctatattaca tgacagtttg gtatgacaat tgatatttac
     1441 agtcgccgga gaacaaccgt ggacgccatg acgcgaatcg agcgctcttt gggtcaggca
     1501 ttcggattgg caaaagggtc gggttgtccc ccctcgctga acgcggcgat gcgctacgct
     1561 gttttccccg gcggtgcgcg cattcgtcca cgattatgcc tcgcggtggc gcgtgcctgc
     1621 ggcgatgaca aaccggaggt tgcggcgagc atggccgccg cggttgaact catgcattgc
     1681 gcctcgttgg tgcacgacga cttgccctgc ttcgatgatg cggcaatcag gcgcggccgc
     1741 gcatcggtgc acaaggcctt cggcgagagg attgcggtac tgaccggcga tgcgctgatc
     1801 gtcctcgcat tcgaggcgct tgcgagaagc gcacaagccg ctccggcgcg tctcgctccc
     1861 ttgatcggca ttctcgcgcg cgccatcggc gcaccgaacg gcatcattgc cggacaagct
     1921 tgggaatgcg aaccgcaaat ctcgctggcc cattatcagc gcgcgaagac cggcgcgttg
     1981 ttcgccgcgg cgaccgccgc aggagcgtgc gcggcgggct gccaggcgca agcctggcac
     2041 ctgctcgggg agcgaatcgg cgaggcttat cagatcgccg atgacattcg cgacgtggtg
     2101 tcgaacgcgc acgagctcgg caaacccacc ggccgcgacc aggcactcgg gcgacccaat
     2161 gccgtccaca agctcggcat gggctcggcc gtgcagcgcc tggaagaatt ggtgagcgcg
     2221 gcgctcgaca ccatcccaga ctgccccggc gcaaacgact tgcgggcgca catactggcg
     2281 gaggccagcc ggttgttacc acgtgaatta gcgcgaccgg cagcctgaag agcgccgccg
     2341 tgccgtggac cgaccgctat ctcagcgtgc gagatcgctg gctgtcgagt ccgcgatttc
     2401 agcgctgggc cgcggcattt ccgctaacgc ggccgacggc gcgtaaacgt gcgcgcgcgc
     2461 tgttcgacgt atgcgcgggg ttcgtctatt cgcaaatcct gttcgcgtgc gtacggctca
     2521 aattattcga tcttttggcg accggaccgc tcacggcgcc gagcatcgcg gcaaagttga
     2581 atttgccgcc ggactcgacc gagcggctgc tggaggccgc cgtgtcgctg cgcttggtcg
     2641 agaagcgcac ggccggacgt ttcgggctcg gcgcattggg cgccgccatg gtcggcaatc
     2701 cggcggtcgg tgcgatggtc gaacaccaca gcctgcttta cgccgattta cacgatcccg
     2761 tgcggctgct gcgcggcgaa caaagccaga ctgcgctcgc ccgatattgg gcgtacgcgc
     2821 attcggacaa acctgcggga ctgccggcgg cgcaaatcgc ggactacacc gcgctgatgg
     2881 gaaactcatt gccattggtc gcggatgaaa tactcgacgc ctatccattg cacaagcacc
     2941 gctccctgct cgatgtcggc ggcggcgacg gcagcttcct gaaggccgcc gcggcgcgct
     3001 ctgaacatct gaaattgatg ctgttcgact tgccgccggt tgcggcgcgt gctgcgcagc
     3061 gctttacaac gctcggtctc gattcgcgcg cgcgcgcggt cggcggcgat tttctcaaag
     3121 acccgcttcc gcacggcgcg gatgtgattt cgctgatccg cgtgattcac gaccacagcg
     3181 atgccgcggc ggccgtggtg ttggccgcgg cgcagcgtgc gttgccgccg aacggggtgc
     3241 tgctgctggc agaacccatg atgggtacgc cgggcgccga gccggtgggc gatgcgtact
     3301 tcggatttta tcttctggcg atgggcagcg gcaggccgcg caccccgcag gcgctgcagg
     3361 caatgctatg cgctgccggc tttgccagtg cgcaactggt gcgtacccgc caacccttgc
     3421 agagccgcct gatcgtcgcg cgcaaagctg ccgatggaaa agtgtcgtga catgttgaca
     3481 tgtttgagag tcagagtatc gtgacactac gcaccatgac ccgcaccgca gaaactccgt
     3541 atccatcgct caacaccctg gccgtggtgt tggagaagcc ggaacagctg gttctgagtc
     3601 ggttgcagct gagtgccccg ggcgacgagg acgtggtcgt cgacatggag tggtcgggta
     3661 tcagcaccgg gaccgaacgg ctgctctggt ccggacgcat gccggcattt ccaggcttgg
     3721 gttatccgtt ggttccaggc tacgaatcgg tgggccgaat cacccatgcg ggtgcacacg
     3781 cagctcggca agtgggcgac cgtgtattcg tgcccggagc acgttgcttc ggcgaagtca
     3841 gagggctttt cggcggcgcc gccgccaaag tcgtggttcc cggtgcgcgg gtgacgccga
     3901 tcagcgagaa catgggcgag cagggcgtat tgctcgcgct cgcggcaacc gcctatcacg
     3961 cagtcacggc gcagaacacg aatctgccgg acttgatcgt cggccacggc gtactcggcc
     4021 gcttgatcgc gcgtttggcg atggcgctcg gcgcagagcc gccgctggtg tgggagcgca
     4081 atcccaatcg catcgacggc agcgtgggct acacggtatt gcatccggac gcggacgtct
     4141 gccgtggcta ccgtgccata tacgatgtga gcggcgacaa caccttgctc gataccttga
     4201 tcgcccgctt ggccccaggc ggcgaaatcg tgctcgccgg tttctacagt cagtcgttgt
     4261 cgttcgcttt tccagcggcg ttcatgcgcg aggcgcgcat ccgaatcgct gccgaatggc
     4321 gtgacggtga tcttaaggcg gtgcggcaat tggtcgactc cgggcggttg cagctcgatg
     4381 gcttgattac tcatcgccgg gaagcgagcg cggcgccggc agcgtaccgc acggcgttca
     4441 ccgatccttc gtgtctcaaa atgattttgg attggagagc ccagtcatga gcaccgcttt
     4501 tgcaaattcg tccgcactgc gctccgagca ggtgcgactc gaggcgctgc gtgccgaagc
     4561 ggcgatcgaa cccgatgccg tgcccagcac cgcgccgacc aagcagacgc aggtcatcgc
     4621 gatttacggc aaaggcggaa tcggcaagag ttttgcgctc gcgaatctgt cctacatgat
     4681 ggcgcaacag ggcaagaagg tgttgctgat cggctgcgac ccgaaaagcg atacgacctc
     4741 gctgctgttc ggggggcgcg cctgtccgac gatcatcgaa acgtcggcca agaaaaagct
     4801 ggcgggcgag ccggtcaaga tcggcgatgt gtgcttcaag cgcgatgggg tattcgccat
     4861 ggaactcggc ggccccgagg tcggccgcgg ctgcggcggc cgcggcatca tccatggttt
     4921 cgagttgctc gaaaaactcg gcttccatga ctggggtttc gactacgtgt tgctcgattt
     4981 cctgggcgac gtggtttgcg gaggcttcgg cctgccgatc gcgcgcgata tgtgtcaaaa
     5041 agtgatcgtt gtcggctcca acgatctgca atcgttgtac gtcgccaata atgtgtgctc
     5101 ggcggtggag tacttccgca agcttggcgg caacgtcggc gtcgccggct tggtgatcaa
     5161 caaggatgat gggaccggcg aagcgcaggc cttcgcggcg gccgcgggca ttccgatcct
     5221 ggcggcgatt cccgccaacg acgatattcg acgcaagagc gccaactacg acatcgtcgg
     5281 tcggccgggc accgagtggg gcccgttgtt cgaggaactg gcggcgaacg ttgcggaggc
     5341 gccgccgctg cgccccaaac cgctgacaca ggaccaattg ctggcgctgt tcaagag
//
LOCUS       DASWZT010000410         1766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_75709, whole genome shotgun sequence.
ACCESSION   DASWZT010000410 DASWZT010000000
VERSION     DASWZT010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75709"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VGE92_11635"
     CDS             <1..520
                     /locus_tag="VGE92_11635"
                     /inference="COORDINATES: protein motif:HMM:NF012684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEY1314524.1"
                     /translation="TETGVHRLVRKSPFDSGNRRHTSFASVFVSPEVDDDIDIEINPA
                     DLRVDVYRSSGAGGQHVNKTESAVRITHLPSGIVVACQGERSQHKNRSTAMKMLKAKL
                     YELEYNKRNAAAKVLEDSKSDVSWGNQIRSYVLDQSRIKDLRTGVEIGNTTAVLDGAL
                     DEFMQASLKQGL"
     gene            528..>1766
                     /gene="lysS"
                     /locus_tag="VGE92_11640"
     CDS             528..>1766
                     /gene="lysS"
                     /locus_tag="VGE92_11640"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY1314525.1"
                     /translation="MSDTDENHLIAERRAKLAKLRERGIAFPNSFRRDALAADIATAY
                     GEKSAAALDAAAVRVKVAGRMRAKRVMGKASFAKLEDSSGAIQIFLQQQTLGEIYDEF
                     KSWDVGDLLGAEGMLFRTKTGELSLRVERLSLLSKSLRPLPDKWHGIADTELRYRRRY
                     VDLIMNEDSRRVFETRSRIVRYLRAFLDARGFLEVETPMLHPIPGGAAARPFKTHHNA
                     LDVDMYLRIAPELYLKRLTVGGFERVYEINRNFRNEGVSTQHNPEFTMLELYQAYADF
                     SDLMEMIETLFQGLADTLVGSRKLIYQGIEIDLSQGFARRSIEDIILANNPDLDPMSL
                     RDTQYLRRVCDQMKIPYKPGDGPGKLQIEIFERTGEHTLVQPTFAYAYPAEVSPLSRR
                     NDVDPFTTDRWEFFVGGRELA"
BASE COUNT          384 a          529 c          535 g          318 t
ORIGIN      
        1 caccgagacc ggtgtgcatc gactggtgcg aaaatcgcct ttcgattcag gcaatcggcg
       61 acatacgtcg ttcgcctcgg tattcgtctc cccggaagtc gacgacgaca tcgatatcga
      121 gatcaatccg gccgatttgc gcgtcgacgt ctaccgctcc agcggagccg gcggacagca
      181 cgtgaacaag accgagtcgg cggtacgcat cacgcacctg ccgagcggga tcgtggtcgc
      241 ctgtcagggc gagcgcagcc aacacaagaa ccgctccacc gcgatgaaga tgctgaaggc
      301 aaagctttac gaactcgagt acaacaaacg caacgcggcg gcaaaagttc tcgaggactc
      361 gaaatccgac gtgagctggg gcaatcagat tcgcagctac gtgctcgacc agtcgcgtat
      421 caaggatctg cgcacgggcg tggaaatcgg caataccacg gcggtgctcg acggcgcact
      481 cgatgaattc atgcaggcca gcctgaaaca aggactatga cgcagcgatg agcgacaccg
      541 acgagaatca tctgatcgcc gaaaggcgcg ccaagcttgc gaagctgcgc gagcgcggca
      601 tcgcatttcc gaacagcttt cgccgcgacg cattggcggc ggatatagcc acggcctacg
      661 gcgagaagag cgctgcggcg ctcgatgccg ctgccgtccg cgtcaaggtg gcaggccgta
      721 tgcgcgccaa acgcgtcatg ggcaaggcga gtttcgcgaa gctcgaggac tcgagcggcg
      781 cgattcagat atttctgcaa cagcagacac tcggcgagat ttacgacgag ttcaagagtt
      841 gggatgtcgg cgatctgctc ggcgccgaag gaatgttatt tcgcacgaaa accggcgagt
      901 tgtcgctgcg agtggagcgg ctgtcgttat tgagcaaatc gctgcggccg ctgcccgaca
      961 aatggcacgg tatcgccgat accgagctgc gttatcggcg gcgctacgtc gatttgatca
     1021 tgaacgagga cagccggcgg gtgttcgaaa cccgctcgcg gatcgttcgc tacctgcgcg
     1081 ctttcctcga tgcgcgcggc tttctcgagg tggagacgcc gatgctgcac ccgatccccg
     1141 gcggtgcagc cgcgcgtccg ttcaagacgc atcacaacgc gctcgacgtg gacatgtatt
     1201 tgcgcattgc gccggaactc tacttgaagc gcctcaccgt cggcggcttc gagcgcgtct
     1261 acgaaatcaa ccggaatttc cgcaatgaag gggtgtcgac gcagcacaat cccgaattca
     1321 ccatgctcga gctctaccaa gcgtatgccg acttcagcga tctgatggaa atgatcgaaa
     1381 cgctgttcca ggggctcgcc gacacgctgg tgggctcgcg caagctgatc taccagggca
     1441 tcgagatcga cctgtcgcag ggtttcgcgc gcaggagcat cgaagacatc attctcgcga
     1501 acaatcccga cttggacccg atgtcgctgc gcgacacgca atatctgcgg cgggtttgcg
     1561 atcagatgaa gattccttat aaacccggcg acggcccggg caaattgcaa atcgagatat
     1621 tcgagagaac cggcgagcac acgttggtgc agccgacctt cgcctacgcc tatcccgccg
     1681 aagtgtcgcc attgtcgcgg cgcaatgacg tcgacccctt caccaccgac cgctgggaat
     1741 tcttcgtcgg cgggcgtgaa cttgcg
//
LOCUS       DASWZT010000411         5524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_37137, whole genome shotgun sequence.
ACCESSION   DASWZT010000411 DASWZT010000000
VERSION     DASWZT010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5524
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37137"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /gene="accB"
                     /locus_tag="VGE92_11645"
     CDS             <1..273
                     /gene="accB"
                     /locus_tag="VGE92_11645"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEY1314526.1"
                     /translation="PATAAGEHKPRNDEYTVTSPMVGTYYSAASPGSKPFVEIGTEIK
                     VGQILCIIEAMKMMNQIESDKAGRVTAMLAKNGEPVEFGQPLFIIE"
     gene            279..1622
                     /gene="accC"
                     /locus_tag="VGE92_11650"
     CDS             279..1622
                     /gene="accC"
                     /locus_tag="VGE92_11650"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEY1314527.1"
                     /translation="MIEKVVIANRGEIALRILRACRELGIKTVAVHSTADFNTKHVLL
                     ADETVCIGPPQSSQSYLNMPAIISAAEVTDSVAIHPGYGFLSENADFAERVEKSGFIF
                     VGPRAETIRLMGDKVSAIKVMKASGVPCVPGSDGPLGNDSDENFRIAKDIGYPVIIKA
                     SGGGGGRGMRMVHTDASLLNAISVTQSEALAAFGNSQVYMEKFLEKPRHIEFQVLADN
                     YGNVVHLGERDCSMQRRHQKVIEEAPAPGLSAKQRKTMGERCARACREVGYRNAGTLE
                     FLYQDNEFYFIEMNTRVQVEHPVTELVTGIDIVKAQLLIAAGEPLPYGQNDIQIRGHA
                     VECRINAEDAKTFVPSPGPIRLWHAPGGPGIRVDSHIYTGYNVPPFYDSMIGKVIAFG
                     DTRDTAIARMRNALAEMVVEGIKTNIPLHQEIFNHSAFRQGGTDIHYLERRLGLK"
     gene            1643..2551
                     /gene="prmA"
                     /locus_tag="VGE92_11655"
     CDS             1643..2551
                     /gene="prmA"
                     /locus_tag="VGE92_11655"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY1314528.1"
                     /translation="MSFYEIEFPLATLSEGMVEAALLDMGASSITLVDGGDQPILEPR
                     PGEMRLWGDTRIRALFEEPPGPDCLDAARHLERLATLFGPHITATARVHAVKNQDWER
                     VWLTDWKSMRFGRRLWVCPTQAEAPDDANAVIVRLDPGLAFGTGTHPTTALCLQILDS
                     LPVAGRTIIDFGCGSGILGIAALKLGAAHVTAVDLDPQALIATRDNAERNGVARNIEV
                     LGVIPGIELSLQPAHCVMANILAGPLIELSPTLTAACEPAGYLLLSGLLKTQAYAVKA
                     AYAAAFDIVRVVEREDWCCIYARRGV"
     gene            2553..3776
                     /locus_tag="VGE92_11660"
     CDS             2553..3776
                     /locus_tag="VGE92_11660"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HEY1314529.1"
                     /translation="MYTQCSKCETVFKLSAEVLRTAGGQVRCGRCGEVFNALARLAED
                     SSAFAMTESPLDLEERADSILESVATLQVAREVAKDNEEFVPPGVEIARLEVLDWPEE
                     DAEQSNITVQADVPTTPQTLEPAPDGGSAHAPAVDAPPGPGTETSTDADPAAAVAQTT
                     QEFDDRSLEFTLPPGELDRIFVESKKRAAKPPLEPILQPEARVPPKPPPPPDMRARHR
                     VGGFAVLENARRDPQRRKSWWLGAAAVLALSLLAQLVHKNRQAIAQGLGPLSVVVRAL
                     YGAVGAPLPAPANLSAYQLRQWGVTGDPDANGTLRLRASILNTAAQLQPYPLLRVTLA
                     DRFGKSIGRREFEPSEYVGKPTARLLAPGERVDASLAILDPGKNAEGFEIDVCLRGVD
                     RKITCANDVAPQAKR"
     gene            3773..4750
                     /gene="dusB"
                     /locus_tag="VGE92_11665"
     CDS             3773..4750
                     /gene="dusB"
                     /locus_tag="VGE92_11665"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEY1314530.1"
                     /translation="MKIGPYELPSRVVLAPMAGVTDRPFRILCRRFGAGLAASEMLSA
                     DVRLWHTPKSRRRMDHTGEPSPRVVQIAGFDPLMMAEAARRNVDAGAQIIDINMGCPA
                     KKVCNRLAGSALMQDEELVARILQSVVRAVDVPVTLKTRTGWDRGHKNGLRIALIAQD
                     SGIQALAVHGRTRTDLYTGIAEHETVAAIKAAVRIPVFANGDVDSPQLARLILDATRC
                     DGIMIGRAAQGRPWIFDEVNFFLATGESRAALALENVRDIMRAHLEDLYAFYGDETGV
                     RVARKHLSWYFRQHPGQDALRNRLVQIETPQEQLSTLLEHYDTSVYRAA"
     gene            4776..5087
                     /gene="fis"
                     /locus_tag="VGE92_11670"
     CDS             4776..5087
                     /gene="fis"
                     /locus_tag="VGE92_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HEY1314531.1"
                     /translation="MVAKKKAKSRRDGSAAFNGRDLRVNGKGVPLRSQAEEALECYFE
                     TLNGHKPAHLYELVMREVEEPLFKVVMGHVQGNQSRAATILGINRGTLRKKLKEFGIN
                     E"
     gene            5095..>5524
                     /gene="purH"
                     /locus_tag="VGE92_11675"
     CDS             5095..>5524
                     /gene="purH"
                     /locus_tag="VGE92_11675"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF014225.4"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY1314532.1"
                     /translation="MLTPVRRALLSVSDKTGIVELARELKERGIELLSTGGTAKLLLE
                     HGISVKEVADHTGFPEIMGGRVKTLHPKIHGGLLGRRGTDDAVMDLHDIEPIDLLAVN
                     LYPFAATVARADCSYDEAVDNIDIGGPAMVRAAAKNHASVT"
BASE COUNT         1112 a         1694 c         1713 g         1005 t
ORIGIN      
        1 cccgccacgg cggccggcga acacaagccg cgcaatgacg agtacacggt cacctcgccc
       61 atggtgggta cctactactc cgccgcatcg ccgggctcca agcccttcgt cgaaatcggg
      121 acggaaatca aggtcggcca gatcctgtgc atcatcgaag cgatgaagat gatgaatcaa
      181 atcgagtccg acaaggcggg gcgggttacc gcgatgctcg ccaagaacgg cgagccggtc
      241 gagttcggcc agccgttgtt catcattgaa tagtcgcgat gattgaaaaa gtcgtcatcg
      301 ctaaccgcgg cgagatcgca cttcgcatcc tgcgcgcctg ccgggagctt ggtatcaaga
      361 cggtcgcggt gcactcgacc gccgacttca ataccaagca cgtgctgctc gcggacgaaa
      421 ccgtttgcat cggtccgccg caatccagcc agtcctacct gaacatgccc gccatcatca
      481 gcgcggctga ggtaaccgac tccgtggcca tccatccggg ctacggattt ttatcggaga
      541 acgcggactt cgccgaacgg gtcgagaaaa gtggcttcat cttcgtcggg ccgcgcgccg
      601 agaccattcg cctgatgggc gataaagtgt ccgcaatcaa ggtcatgaag gcctcgggcg
      661 tgccctgcgt gccgggctcg gacggaccgc tcggcaacga ttcggacgag aacttccgaa
      721 ttgccaaaga catcggctac ccggtaatca tcaaggcttc cggcggcggt ggcggccgcg
      781 gcatgcgaat ggtgcacacc gacgcctctt tgctcaacgc catcagtgtc acccaatcgg
      841 aagcgctcgc cgcattcggc aactcccagg tctacatgga gaaattcctg gagaagccgc
      901 ggcatatcga gttccaggtt ctagccgaca attacggcaa tgtggtgcac ttgggcgagc
      961 gcgactgctc gatgcagcgg cgtcaccaaa aggtgatcga agaggcaccc gcccccggct
     1021 tgtcggccaa gcaacgcaag accatgggcg agcgctgcgc aagagcctgc cgcgaagtcg
     1081 ggtatcgcaa cgcgggtacg ctcgagttcc tgtaccagga caacgagttc tacttcatcg
     1141 aaatgaatac gcgcgttcag gtcgagcatc cggtcaccga acttgtcacc ggaatcgata
     1201 tcgtcaaagc gcagctgctc attgccgcag gtgagccgtt gccttacggc cagaacgaca
     1261 ttcagattcg cggccatgca gtcgagtgcc ggatcaacgc tgaggacgcc aagaccttcg
     1321 tgccgtcacc cggcccgatc cgattgtggc atgcgcccgg cggccccggc atccgggtcg
     1381 acagccacat ctacaccgga tacaacgtgc cgccgttcta cgactccatg atcggcaagg
     1441 tcatcgcctt cggcgacact cgagatacgg cgatcgcccg catgcgcaat gcattggcgg
     1501 aaatggtcgt cgaaggcatc aagaccaaca ttcctctgca ccaagagata ttcaaccact
     1561 ccgcttttcg ccagggcgga accgatattc actatttaga gcgacgcttg ggattgaagt
     1621 gaaaccctag ggtcttgcag cgatgtcgtt ctacgaaatc gaatttccgc tcgcgacgtt
     1681 gagcgaagga atggtcgagg ccgcgctgct cgacatgggc gcaagttcga tcaccctggt
     1741 cgacggcggc gaccaaccga tactcgagcc caggcccgga gaaatgaggc tctggggcga
     1801 cacccgcatc cgcgcgttgt tcgaggaacc cccaggtccg gactgcttgg acgcggcccg
     1861 ccatctcgag cggctcgcca ccctattcgg gccgcacatc accgcgaccg cacgcgtgca
     1921 cgccgtgaag aatcaggact gggaacgggt gtggctcacc gactggaaat ccatgcgctt
     1981 cggccggcgg ctgtgggtgt gcccgacgca ggcagaagca ccggatgacg cgaacgccgt
     2041 catcgtgcgg ctcgatcccg gcctggcgtt cggcaccggc acgcatccca ccacggcact
     2101 gtgtctacag atattggatt cattgccggt cgcgggccgc accatcatcg actttggctg
     2161 cggttcaggc attttaggaa ttgccgctct gaaactcggt gcggctcacg tgacggcggt
     2221 ggatctggat ccccaagcgc tgatcgcgac gcgcgacaac gccgagcgca acggcgtcgc
     2281 tcggaacatc gaagtactgg gggtgattcc cgggatcgag ctcagtttgc agccggcgca
     2341 ctgcgtcatg gcaaacattc tcgccgggcc gctgatcgaa ttgtcgccga cacttaccgc
     2401 ggcctgcgag ccggcgggat acttgctgct gtccgggctg ctcaagacgc aggcctatgc
     2461 ggtaaaagcc gcgtacgccg ccgcttttga tatcgtgcgg gtggttgagc gcgaagattg
     2521 gtgttgcatt tacgctcgga gaggcgtcta gcgtgtatac gcagtgttca aaatgcgaga
     2581 cggtcttcaa gttgtccgcg gaggtgttgc gcacggccgg cggtcaggtg cgctgcggaa
     2641 gatgcggcga ggtgttcaac gccttggcgc gcctcgccga agattcgagc gccttcgcga
     2701 tgaccgagtc gccgcttgat ctcgaggaac gcgccgacag cattctcgag tccgtggcta
     2761 ccttgcaggt agcccgggaa gtcgccaagg acaacgagga gttcgtccct ccgggggtcg
     2821 agatcgcgcg gctcgaagta ctcgattggc ccgaggaaga cgccgagcaa tcgaatatca
     2881 cagtgcaagc ggacgtgcct acgacgccgc agacactgga accggcgccg gacggcggct
     2941 ccgcgcatgc gccggccgtc gacgcgccgc ccggccccgg gaccgagact tcgacggacg
     3001 ccgaccctgc cgcggctgtg gcgcaaacga cgcaagaatt cgacgaccgc tcgctcgagt
     3061 tcacattacc gcccggcgag ctggatcgga tttttgtcga gtcaaaaaaa cgcgcggcga
     3121 agccgccgct cgagccgata ctgcagcctg aagcgcgtgt gccgccgaag ccgccaccgc
     3181 cgcccgacat gcgcgcccgc catcgcgtcg gcggattcgc ggtgctcgag aatgcgcgcc
     3241 gcgacccaca gcgccgcaaa tcctggtggc tcggcgccgc ggccgtgctg gcgctgtcgc
     3301 tgttggcgca gctcgtgcat aagaatcgcc aagcaatcgc ccagggcctc ggccccttga
     3361 gcgtcgtggt acgcgccctg tacggggcgg tgggtgcccc gttgcccgcg ccggcgaatt
     3421 tgtccgccta ccaactacgc caatggggcg tcaccggcga tccggacgcc aacggcacgt
     3481 tgcggctgcg cgcaagcatc ttgaacaccg ccgcgcagct gcaaccgtat ccgttactgc
     3541 gcgtgacgct cgccgatcgc ttcggcaaga gcatcggccg gcgcgaattc gaacccagtg
     3601 aatacgtggg caagccaacc gcgcggctgc ttgcgcccgg cgagcgcgtc gatgcgagtt
     3661 tggcgattct cgatcccggc aagaacgcgg agggtttcga aatcgatgta tgtctgcgcg
     3721 gcgtcgacag aaaaatcaca tgcgcaaacg atgttgcgcc gcaagccaag cgatgaagat
     3781 cggtccgtac gaattgccct cgagagtggt gctggcgccg atggcgggag tcaccgatcg
     3841 tccgtttcgc attctgtgcc ggcgctttgg cgcaggactt gctgcatctg aaatgctgag
     3901 cgccgacgtg cgcctgtggc acacgccgaa atctcggcgg cgcatggatc acactggcga
     3961 gcccagcccg cgcgtggtgc agatcgccgg cttcgatcct ctgatgatgg ccgaagcggc
     4021 gcgccgcaac gtcgatgccg gggcgcagat cattgatatc aacatgggtt gtcccgccaa
     4081 gaaagtttgc aatcgcctcg cgggctccgc tttgatgcag gatgaggagt tggttgcacg
     4141 aattttgcaa agcgtggtgc gtgcggtcga cgtgccggtc acgctcaaga cgcgcaccgg
     4201 ctgggatcgc gggcacaaga acggtttgcg cattgcactg attgcgcaag acagcggtat
     4261 tcaagcactt gcagtgcacg gacgcacgcg cacggatctt tatacaggaa ttgccgaaca
     4321 tgaaacggtc gctgcgatca aagccgcggt aaggataccc gtgtttgcga acggcgatgt
     4381 cgattcgccg caactcgctc gtctaatcct cgacgccacc cgttgcgacg gcatcatgat
     4441 cggccgcgcg gcgcaaggcc ggccatggat cttcgacgaa gtcaattttt tcctcgcgac
     4501 cggtgaatcg cgcgccgctc tggcgttgga aaatgtccgt gatattatgc gcgcccacct
     4561 cgaagacctg tacgcctttt atggcgatga aaccggtgtt cgtgtggcac gtaaacattt
     4621 gagttggtat tttcgacagc atcctggaca agacgcattg cggaatcgac tggtgcagat
     4681 cgagactccg caggagcaat tgtcgacgct gctggagcat tacgacacga gtgtgtatcg
     4741 cgctgcgtag cgcgcagggg agttcggacg aggccatggt cgcaaaaaag aaagcaaaat
     4801 cacgcaggga tggatcggcg gctttcaacg gccgcgatct gagggtcaac gggaaaggtg
     4861 tgcctttgcg ttcccaggcc gaagaggcct tggagtgcta tttcgaaacc cttaacggtc
     4921 acaagcctgc gcatttgtac gaactcgtga tgcgagaagt ggaggaaccg ctgttcaaag
     4981 ttgtcatggg ccacgtgcag ggcaaccaga gccgcgcagc cacgattctc ggcatcaacc
     5041 gcggcacctt gcgcaagaag ctcaaagagt tcggtatcaa cgagtagcgt tcaattgctc
     5101 acgcccgtcc gccgtgcgtt gctcagcgtc tccgataaga ccggaattgt tgaactcgcg
     5161 cgagaattga aggaacgcgg cattgagctc ctctcgaccg gcggaaccgc caagctcttg
     5221 ctcgaacacg gcatttccgt caaggaggtg gccgatcaca ccggatttcc ggaaatcatg
     5281 ggcggtcgcg tcaagactct gcatcccaag attcacggcg ggttattggg caggcgcggc
     5341 accgatgacg ccgtcatgga tttgcacgat atcgagccca tcgatttgct cgccgtcaat
     5401 ctttatcctt tcgccgcgac cgtggcacgg gccgactgta gctacgacga agcggtcgac
     5461 aatatcgata tcggcggccc ggcgatggtc cgcgccgcgg ccaagaatca tgcctcggtc
     5521 accg
//
LOCUS       DASWZT010000412         4020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_95369, whole genome shotgun sequence.
ACCESSION   DASWZT010000412 DASWZT010000000
VERSION     DASWZT010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95369"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VGE92_11680"
     CDS             <1..528
                     /locus_tag="VGE92_11680"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY1314533.1"
                     /translation="AAAHFISQGYKNIGAIAAGRTEDMVDFRGDARLESFEEALKTAG
                     LRTDLVLRHGEPPVSYAHGAAGLSILLEREPKLEAVFAISDVAAVGLMMECRRRRIDI
                     PRGLAVIGFGDFDIAAEMEPPLTTIGVKFDELGRETGILIVDLLRGLKPPESRVTNVG
                     VALIRRGTTLARAGS"
     gene            complement(525..1478)
                     /locus_tag="VGE92_11685"
     CDS             complement(525..1478)
                     /locus_tag="VGE92_11685"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEY1314534.1"
                     /translation="MSEPASTMHTAALQMNALSPLLEQELASRFQVYRWFDILDHERF
                     LSEHAGSIRAVVTGGHIGLPNELLERLPALQIVAINGVGYDKVDLEAARRRGVCVSRT
                     AGVLTDDVADLAVGLVIALLRGIVSADRLVRGGSWPAGEAPLARKVSGKRFGIVGLGN
                     IGAAIAARLAAFGPVAYCNRSAKPVPYRFVSSVNELASMSDVLILAASSNASTRGMVG
                     REILESLGPSGYLVNVARGALVDEAELIAALATQRIAGAALDVFIDEPHVPEALRSAA
                     NAVLTPHIASATVETREAMARAVLANLDAFFAGHPVPGAID"
     gene            complement(1475..2236)
                     /locus_tag="VGE92_11690"
     CDS             complement(1475..2236)
                     /locus_tag="VGE92_11690"
                     /inference="COORDINATES: protein motif:HMM:NF005711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY1314535.1"
                     /translation="MDLFDLAGRRALITGSTQGIGFALAQGLGRAGASVLLNGRDGTR
                     LAGAVARLRERGIATEGALFDVTDPAAVERGIAEAEQNFGAIDILVNNAGIQRRQPLE
                     DFPVAAWREVMTTNVDSVFYVGQAVARRMIPRGRGKIINIASLQSEAARPGIAPYTAS
                     KGAVKNLTKGMCADWARHGLQINAVGPGYFETPLNQALIDDPVFDAWLKKRTPAGRWG
                     KVEELQGAVIFLASRASDFVNGQTLYVDGGVLAVL"
     gene            complement(2274..3254)
                     /locus_tag="VGE92_11695"
     CDS             complement(2274..3254)
                     /locus_tag="VGE92_11695"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY1314536.1"
                     /translation="MRYRQLGGLEWQVSEIGFGGWAVGGGWGTQTDQDSIAALHRSFD
                     LGCNFVDTAQNYGNGHSERVIAQALRERGKSERIYVATKIAPQAGAWPPSPYDRIEDR
                     FSATHVRERVERSLRDLGTDCLDLVQLHTWTRAWNKNPHAFETLRELRKEGKLRAIGV
                     STPEHDQNSVIDLMREKWVDTVQVVYNIFEQEPQAELFPAAQQSHVGVIVRVALDESA
                     LTGKLTADTRFADGDIRNRFFAGDRLARTVQRVEAIRQTVGDEEPSLATAALKFALKP
                     AAVSVVIPGIRSVAQAEMNLAVGSAPPLGDALEERLRPHAWRRGFWYAGK"
     gene            complement(3256..3939)
                     /locus_tag="VGE92_11700"
     CDS             complement(3256..3939)
                     /locus_tag="VGE92_11700"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY1314537.1"
                     /translation="MTDHAALLARVAALEAQLARLDAARELHNLMGQYTVNFSPKYMH
                     RAVDFYALERPDVSVEIADRGVFVGGDAIRKLFGAQFAMSLKGNLLVHYLATPMIEVA
                     RDGQSAKGVWRSPGIEAVVPPAGGKPVALWSFGTYAVDFIRVRGEWKILHLHWFRIVK
                     CSYEAGWVDDLSMTYTGKLPDSAHVRPSTYHNPYTPDNIQEPIPPCPQPFDTMTDANW
                     MVQAHQAGG"
BASE COUNT          709 a         1331 c         1271 g          709 t
ORIGIN      
        1 gccgccgccc acttcatctc gcaaggctac aagaacatcg gggccatcgc ggccggccgt
       61 accgaggaca tggtcgattt tcgtggcgac gcgcggcttg aaagtttcga ggaggcgctc
      121 aagaccgcgg gtctgcgcac tgatctcgtt ctgcgtcatg gcgaaccgcc ggtatcctac
      181 gctcacggtg cagcgggctt gtccattctg ctcgagcgcg agccgaagct cgaagccgtg
      241 tttgcgatct cggatgtcgc ggccgtcggg ttgatgatgg agtgccgccg gcgccggatc
      301 gacattcctc gagggctcgc ggtcatcggg ttcggcgatt tcgacatcgc ggcggaaatg
      361 gaaccgccgc tgacgaccat cggcgtgaaa ttcgacgagc tcggccgtga gaccggcatc
      421 ctgatcgttg atctgttgcg agggttgaag ccgcccgagt cccgggtcac gaacgtgggc
      481 gtggcgttga ttcgtcgcgg aacgaccctg gcgcgagcgg gctcctagtc gatcgcaccc
      541 ggtaccgggt ggcctgcgaa gaacgcgtcg aggttggcga gcaccgcacg agccatcgcc
      601 tcacgggttt ccaccgtcgc gctcgcgatg tgcggcgtca gcaccgcgtt cgcagcgctg
      661 cgcagcgcct cgggcacgtg gggctcgtcg atgaatacgt cgagcgccgc tccggcgatg
      721 cgctgggttg cgagcgccgc gatcagctcg gcttcgtcga cgagtgcacc gcgagccaca
      781 ttgaccaaat aaccgctggg cccgagcgac tccaaaatct cgcgcccgac catgccgcgg
      841 gtggatgcat tggagctcgc cgcgagaatc aacacatcgc tcatgcttgc gagctcattg
      901 accgaactga cgaatcggta tggcaccggc ttcgcgctgc gattgcagta ggcgaccgga
      961 ccaaaggcgg cgaggcgcgc ggcgatggcg gcgccgatat tgccgaggcc gacgatgcca
     1021 aagcgtttac ccgacacctt gcgcgccagc ggcgcctcgc ccgccggcca cgatccgccg
     1081 cgtaccaatc gatccgcaga cacaataccg cgcagcagag cgatcacgag tccgaccgcg
     1141 agatccgcca catcgtcggt gagtacgccg gccgtgcggc tcacgcacac gccgcgccgc
     1201 cgggccgcct ccagatccac cttgtcgtag ccaacgccgt tgatggcaac gatctgcagc
     1261 gccggcaggc gctcgagcag ttcattcggc aggccgatgt ggccgcccgt gacgaccgct
     1321 cggatcgaac ccgcatgctc gctcaagaag cgctcgtgat ccaggatgtc gaaccagcga
     1381 tatacctgaa agcgcgacgc gagttcctgc tcgagcagcg gcgagagcgc attcatttgc
     1441 agcgccgcag tgtgcatggt gctcgccggc tcgctcatag cacggccagt acgccgccat
     1501 cgacatagag agtctggcca ttcacgaagt cggaggcacg cgatgccagg aaaatgaccg
     1561 caccctgaag ttcttcgacc ttgccccagc ggcccgccgg cgttcgcttc ttgagccacg
     1621 cgtcgaaaac gggatcatcg atcagcgcct ggttcaaggg ggtttcgaaa tagcccggtc
     1681 cgaccgcgtt gatctgcagt ccgtgccgtg cccaatccgc gcacatgccc ttggtcaaat
     1741 tcttgaccgc gcccttcgac gcggtgtaag gcgcgattcc gggccgcgcc gcttcgcttt
     1801 gcagactcgc aatattgatg atcttgccgc ggccgcgcgg gatcatgcgg cgcgcgacgg
     1861 cttggccgac gtagaaaacg ctatccacgt tggtcgtcat gacttcacgc cacgcggcaa
     1921 cgggaaagtc ttcgagcggc tggcggcgct ggatccccgc gttgttcacg agaatgtcga
     1981 tggcaccgaa attctgctcc gcctcggcaa taccgcgttc gacggcggcg ggatcggtga
     2041 cgtcgaacag cgcgccctcg gtcgcgatgc cgcgctcgcg caggcgcgcg acggcgcccg
     2101 ccagccgcgt gccgtcgcgc ccgttgagca gcacgctcgc accggcgcgg ccgaggccct
     2161 gcgcgagcgc gaaaccgatt ccctgggtgg acccggtaat caacgcgcga cggcccgcga
     2221 ggtcaaacaa gtccacggtc gattccctgc ggctgcgtca ccggcgagtc tccctacttt
     2281 ccggcgtacc aaaagccgcg gcgccaagcg tgcggcctca ggcgctcttc cagcgcgtcc
     2341 ccgagcggcg gcgctgagcc gaccgccaga ttcatctcgg cctgcgcgac actgcgaatt
     2401 ccggggatca ccaccgagac cgcagcgggc ttgagcgcga acttgagcgc tgccgtcgcg
     2461 aggctgggct cctcatcgcc gacggtctgg cgaatcgcct cgacgcgctg cacggtgcgc
     2521 gcgagtcgat ctccggcaaa gaagcgattg cggatatcgc cgtccgcgaa tcgcgtgtcc
     2581 gccgtcagct tgccggtgag ggcactctcg tcgagtgcga cgcgcacgat gacgccgacg
     2641 tggctttgct gcgcggcggg aaacagttcc gcctgcggct cctgttcgaa gatgttgtag
     2701 acgacttgaa cggtgtcgac ccatttttca cgcatcaggt cgatgacgga gttctggtcg
     2761 tgctccggcg tgctgacgcc gatcgcccgt aacttgcctt ccttgcgcag ttctcgcagc
     2821 gtttcaaacg cgtgcggatt cttgttccaa gcgcgcgtcc aggtatgcag ctgtacgagg
     2881 tcgaggcaat cggtgcccaa gtcgcgcagg ctccgttcaa cgcgctcgcg cacgtgcgtt
     2941 gcgctgaatc gatcctcgat ccggtcatag gggctcggcg gccaagcgcc ggcctggggc
     3001 gcaatcttgg tcgcgacata aatccgctcg ctcttgccgc gttcgcgcaa ggcttgcgcg
     3061 atcacgcgct cgctatgccc attgccgtag ttctgcgccg tgtcgacgaa attgcaaccg
     3121 aggtcgaaag agcgatgcag cgccgcgatc gaatcctggt ccgtctgtgt gccccagccg
     3181 ccgccgactg cccagccgcc gaagccgatt tcggagacct gccactcgag cccgcccaac
     3241 tgacgatatc tcatgtcaac ctcccgcctg gtgcgcctgc accatccagt tcgcgtccgt
     3301 catcgtatcg aacggttgcg gacagggcgg gatcggctct tggatgttgt ccggcgtgta
     3361 cggattgtgg taggtcgagg gccgaacgtg cgccgagtcg ggcaacttgc cggtgtaggt
     3421 catcgacaga tcgtccaccc atccagcctc gtagctgcac ttgacgatgc gaaaccaatg
     3481 cagatgtaga attttccact caccgcgcac gcgaatgaag tcgaccgcat aggtgccgaa
     3541 tgaccacagc gcgaccggtt tgccgccggc gggcggtaca accgcctcga ttcccggcga
     3601 gcgccacacg cctttcgcgg attgaccatc gcgggccacc tcgatcatcg gtgtcgccag
     3661 atagtgaacc agcagattgc ctttgaggct catcgcgaat tgagcaccga acaacttgcg
     3721 aatcgcatcc ccgcccacga acaccccgcg atcggcgatc tcgaccgaca cgtcgggtcg
     3781 ttcgagggcg tagaagtcga ccgcacggtg catgtatttg gggctgaaat tgaccgtgta
     3841 ctgccccatg agattgtgca gctcccgtgc ggcatcgaga cgcgcgagtt gcgcttcgag
     3901 cgcggcaaca cgggcgagca gagcggcgtg atcagtcatg acaagaacct ccaaacattg
     3961 aatggctact ccgccgcggt caaaccgccg tccacgggca gcagcacacc gttgacataa
//
LOCUS       DASWZT010000413         2682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_66132, whole genome shotgun sequence.
ACCESSION   DASWZT010000413 DASWZT010000000
VERSION     DASWZT010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66132"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VGE92_11705"
     CDS             complement(<1..575)
                     /locus_tag="VGE92_11705"
                     /inference="COORDINATES: protein motif:HMM:NF012691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314538.1"
                     /translation="MAKIPPGVGPRFPQVVVLVGATGDLARRKLLPGMFHLASAGFIP
                     GCRIIGVSLDDMDADGFRKLAREALTEFSTRKVADADWETFANNLQYVPMSAGAQALK
                     AAVVEAEGSFNSEVGRMHYLSVPPAAALSAVRLLGEAGLVERSRVIMEKPFGTDLETA
                     VALNAKLHEVFDEDRIFRIDHFLGKEAAQNIL"
     gene            complement(613..1368)
                     /locus_tag="VGE92_11710"
     CDS             complement(613..1368)
                     /locus_tag="VGE92_11710"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY1314539.1"
                     /translation="MRMTESLERLRDVGYAVVPQFVPHEQLSALNAAARSQLAARSEP
                     LEFEADLRYPGAPESRQAAGGETVRRLLDAYARATEFARCATAPELQEWMTAYFDEDV
                     LLSRVHHNCVMTKHPRYGSLTHWHRDIRYWSFSRDDLVSVWLALGVEQIENGALWFVP
                     GSHRMALGADRFDEAKFLRADRADNELVLRGAESPRLAPGDAVFFHCNTLHSAGKNSS
                     DNVKFSLVFTYHARSNAPQPGTRSASKAEVRLG"
     gene            complement(1359..2465)
                     /locus_tag="VGE92_11715"
     CDS             complement(1359..2465)
                     /locus_tag="VGE92_11715"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF010006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEY1314540.1"
                     /translation="MTAPILLTPGPLTTSQATKSAMLADFGSWDGSFNELTESVCRDL
                     TAIVNAQDEHVCVPLQGSGTFAVEAALGTLVPRRGKVLVPDNGSYCKRIARILGYLER
                     QTVVLAHEENEPANLARIDAALAADPAITHVAQVHCETGTGILNPLPEIAELVASRGR
                     ALIVDAMSSYAAIPIDARTLRFDALIAASGKCLEGVPGMGFIIARRTALERSVGNSRS
                     LVMDLLDQWQYMCKTGQWRFTPPTQVVAALRAAIDQFQAQGGQPARLARYTENCTALV
                     SAMRALGFKTFLPDALQAPIIVTFHTPPDPAYDFGNFYRRVRDRGYILYPGKLTAVET
                     FRVGCIGAIDAGTLREAVAAIGEALREMGVRRCA"
     gene            complement(2473..>2682)
                     /locus_tag="VGE92_11720"
     CDS             complement(2473..>2682)
                     /locus_tag="VGE92_11720"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY1314541.1"
                     /translation="SSLCTNRLDLIARFIQELHVGSVNVREVPGYRLEVTPFGGIKDS
                     GLGYKEGVQEAMKSYCNTKTYSLPW"
BASE COUNT          493 a          862 c          845 g          482 t
ORIGIN      
        1 agaatgttct gcgccgcttc cttgccgagg aaatgatcga ttcggaaaat tcgatcctcg
       61 tcgaacacct cgtgcagttt cgcattcagt gcaaccgcag tttcgaggtc ggtgccgaaa
      121 ggcttttcca tgatgaccct ggagcgctcg accaagcccg cctctcccag cagccgtacc
      181 gcggacagcg ctgccgccgg cggcacactc aggtagtgca tccggccgac ttcgctgttg
      241 aagctacctt cagcctcgac aaccgccgcc tttagagctt gcgcgccggc agacatcggc
      301 acgtactgca ggttgttggc gaacgtctcc cagtccgcgt ccgccacctt gcgggtcgag
      361 aactccgtga gagcctcgcg cgccagcttg cgaaaaccgt ctgcatccat gtcgtcgagc
      421 gacacaccga tgatgcggca gcccgggatg aacccggcac tcgcgagatg aaacatgccg
      481 ggcaacagct tgcgccgggc caggtcaccc gtcgcgccga ccagcacgac gacttgcggg
      541 aatcgaggac ccacaccggg aggtatcttt gccactgttt cacatcctgt cgaggttgca
      601 ccggcggcta gtctagccga gtctgacctc ggccttcgac gctgatcgcg tgcccggttg
      661 cggtgcattg ctccgggcat ggtaagtgaa caccagcgag aacttcacgt tatcgctcga
      721 attcttgccc gccgagtgca gggtgttgca atggaagaac acggcatcgc ccggtgcgag
      781 ccgcggcgac tcggcgccgc gcagaaccag ctcgttatcc gcgcgatcgg cgcgcagaaa
      841 cttcgcctca tcgaaccgat cggcgccaag ggccatgcgg tgcgagcctg gcacgaacca
      901 aagcgcgcca ttctcgattt gctcgacgcc gagggcaagc cagaccgaga cgagatcgtc
      961 gcggctgaat gaccaatagc gaatatcgcg atgccagtgg gtgaggctgc catagcgggg
     1021 atgcttggtc atgacgcaat tgtgatgaac tcgtgacaac agcacgtcct cgtcgaagta
     1081 ggcggtcatc cattcttgga gttccggcgc ggttgcacag cgtgcgaatt cggtggcacg
     1141 agcatacgcg tccagcaagc ggcgcacggt ctcgccgccg gcggcttggc gcgactcggg
     1201 agctccggga tagcgcagat ctgcctcgaa ttcgagcggt tcgctgcgcg ccgcgagttg
     1261 cgatcgcgcc gccgcgttga gcgcgctcag ctgctcgtgc ggcacgaatt gcggcacgac
     1321 tgcatagccc acatcgcgca accgctcgag gctctcggtc atgcgcatcg gcgcacgccc
     1381 atctcccgca gtgcctcgcc gatcgcggcc acagcctcgc gcaatgtgcc ggcgtcgatg
     1441 gcgccaatac acccgacgcg gaaagtctcg acggcggtca atttgcccgg atacagaatg
     1501 tatccgcggt cgcgcacgcg ccgatagaaa ttgccaaagt cgtaggccgg atcgggcggc
     1561 gtgtggaatg tcacgatgat cggcgcctgc agcgcgtccg gcaggaaagt cttgaagccc
     1621 agcgcccgca ttgccgacac caaggcggtg cagttttccg tgtagcgcgc gagccgcgcc
     1681 ggctggcccc cttgtgcctg gaattggtcg atggcggctc gcagcgccgc gacgacttgg
     1741 gttggcggcg tgaagcgcca ctggccggtc ttgcacatgt actgccactg atcgagcaaa
     1801 tccatgacca aggagcggct attgccgacc gagcgctcga gcgcggtgcg ccgggcgatg
     1861 atgaagccca tgcccggcac gccctcgagg cacttgccgg aagccgcgat cagcgcatcg
     1921 aagcgcaagg tccgagcatc gatcgggatg gcggcataag agctcatcgc atcgacgatc
     1981 agcgcgcgtc cgcggctcgc aacgagctcg gcaatctcgg gcaaagggtt gaggatcccg
     2041 gtcccagtct cgcagtgcac ctgcgcgacg tgcgtgatcg ccgggtcagc ggccaaagcg
     2101 gcgtcgattc gcgccaagtt cgcgggctca ttttcctcgt gtgccagcac caccgtctgc
     2161 cgctccaggt aacccaagat ccgcgcaatg cgcttgcagt agctgccgtt gtcgggtacc
     2221 aacaccttgc cgcgcctcgg taccagcgtg ccgagtgcgg cttcgacggc gaacgtgccg
     2281 ctcccctgca gcggcacgca aacatgctca tcttgtgcat tgacgattgc agtgaggtcg
     2341 cggcataccg attcggtgag ttcgttgaag ctgccgtccc acgagccgaa gtcggccagc
     2401 atggcggact tggtggcttg cgaggtggtg agcgggcctg gcgtgagcag tatcggcgcc
     2461 gtcatgagtt cctcaccatg gcaacgaata cgtcttggtg ttgcaatagc tcttcatggc
     2521 ttcctgtacc ccctccttgt aaccgagccc ggagtccttg atgccgccga acggtgtcac
     2581 ctccaggcgg taaccgggaa cctcccgcac attgacgctg ccgacatgca gctcttggat
     2641 gaagcgcgcg atcaagtcga ggcggttcgt acagagcgag ga
//
LOCUS       DASWZT010000414         3206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_13152, whole genome shotgun sequence.
ACCESSION   DASWZT010000414 DASWZT010000000
VERSION     DASWZT010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13152"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="VGE92_11725"
     CDS             complement(<1..867)
                     /locus_tag="VGE92_11725"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF009395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEY1314542.1"
                     /translation="MSNLQDRLQNVNVASSDLLATPEEVKRRLPLTERAAQTVFKARE
                     TVRAILERRDPRLFVVVGPCSIHDVVAARDYATRLKALADKVEATLLPIMRVYFEKPR
                     TTVGWKGLINDPDLDDSFHIEKGILMARELLLFVAQMGLPAGTEALDPIMPQYLSELI
                     TWTAIGARTTESQTHREMASGLSTPVGFKNGTDGALATSINALQSVRHPHHFLGITQQ
                     GQSAVFRTRGNAHAHLVLRGGGGRVNYDAVSIALAERELTAAGLAANVVVDCSHGNSN
                     KDPSLQPQVAENC"
     gene            complement(876..1733)
                     /gene="kynA"
                     /locus_tag="VGE92_11730"
     CDS             complement(876..1733)
                     /gene="kynA"
                     /locus_tag="VGE92_11730"
                     /EC_number="1.13.11.11"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase"
                     /protein_id="HEY1314543.1"
                     /translation="MQEPTPQPKPFVDLSDEAVHWDLTDALTYSQYLDLDKLLDAQHP
                     LSYQHDEMLFIVIHQVSELWMKLCLHELGATVACVQRDDLGPTFKMLARVSTIQQQLL
                     QSWDVLATITPSDYSAFRNSLGKSSGFQSPQYRLLEFLIGNKNADTIKVFRGDPQTYA
                     NLERALRAPSLYDEVLRLLSRRGLSVPPEAIDRDFTLPYQASKQVAAAWLSVYHNSVK
                     EWDLYELAERLVDLDYKFQLWRFAHVKTVERIIGYKRGTGGTGGVSYLTKALELKFFP
                     ELWTIRTSM"
     gene            complement(1736..2464)
                     /locus_tag="VGE92_11735"
     CDS             complement(1736..2464)
                     /locus_tag="VGE92_11735"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEY1314544.1"
                     /translation="MKPQGTQLSLFETRSSSDPFAVRVSPRARRLTARVHVGGRVEIV
                     VPLGVNAHTVRDFVQRFTPWIDRKVAAMQCFAASSEPVPTRVDFACSGETFLVDWRRG
                     AKRVLEESPGRLSVQAADERGARFLLQSWLKRAAFERLAPRLLQLAQELNCQVARVSI
                     RCQRTRWGSCSTRGTVSLNCSLLFLDFDVVRYLFVHELAHTKHMNHSASFWRLVEKIE
                     PDYRRLDRELLAGWRTVPGWVFKT"
     gene            complement(2484..>3206)
                     /locus_tag="VGE92_11740"
     CDS             complement(2484..>3206)
                     /locus_tag="VGE92_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314545.1"
                     /translation="MLCALLCWAAAASAQQGGDLQAQILYAYQTEDDNGLRNLIRDLS
                     TQVKSDEGDLALHYHLAHAQYRRGLLAERSAQREAESAYGACIDELKPLLAQDVKSVE
                     ALVLQSACYAGAANHEKLEAVLLRSRADERLKTALRLEPRNPRALYVSGSQGQAAKPG
                     SAERQQAFAQLQLAVQLFDASSATRIDTPGWGHADAYLAMGLELKARGDQVGARNYIE
                     KSLIAAPDFKAAQRQLAQLARP"
BASE COUNT          594 a         1043 c          992 g          577 t
ORIGIN      
        1 gcagttctcg gcgacctgcg gctgcaaact gggatccttg ttggaattgc cgtggctgca
       61 atcgaccacc acgttcgccg ccaatccggc cgccgtcagc tcacgctccg ccaaggcaat
      121 gctcaccgcg tcgtagttga cccggccgcc gccgccgcgc agcaccaggt gtgcgtgcgc
      181 gttgccgcgg gtgcggaaca ccgctgactg tccctgctgg gtgatgccga gaaaatggtg
      241 cggatggcgt accgactgca aggcgttgat cgaggtcgcg agtgcgccgt cggtgccgtt
      301 cttgaatccc accggggtcg atagaccgct cgccatttcg cgatgcgtct gcgactctgt
      361 ggtgcgggcg ccgatggccg tccaagtgat gagctcggac aggtattgcg gcatgatggg
      421 atcgagcgct tcggtgccgg ccggcagtcc catctgcgcc acgaacaaca acaactcgcg
      481 cgccatcaaa atgcccttct cgatgtgaaa cgagtcatcg aggtcggggt cgttgatcaa
      541 tcctttccag ccgaccgtgg tgcgcggctt ctcgaagtac acgcgcatga tgggcagcag
      601 cgtcgcttcg actttgtcgg ccagagcctt gagccgggtc gcataatcgc gtgcggcgac
      661 cacgtcgtga atcgaacacg ggccgaccac cacgaacagc cgcggatcgc gccgctccaa
      721 tatcgcgcgc accgtctcac gcgccttgaa tacggtctgc gccgcgcgct cggtcagcgg
      781 cagccgccgc ttgacctcct ccggcgttgc cagaaggtcg ctggaagcga cattgacgtt
      841 ttgcagtcgg tcttgaagat tactcacggc aatgcctaca tcgaagtccg gatggtccac
      901 agctccggaa agaacttcag ttccagcgcc ttggtcaagt atgacacgcc gcccgttccg
      961 cccgtgccgc gcttgtagcc gatgatgcgc tcgaccgtct tgacatgggc gaagcgccac
     1021 agctggaatt tgtaatcgag gtcgacgagg cgctccgcga gctcgtataa atcccactcc
     1081 ttgaccgagt tgtgatagac gctgagccat gccgccgcca cctgtttgct cgcttgatag
     1141 ggcagcgtga aatcacggtc gatggcctcc ggcggcaccg acaagccgcg ccggctcaac
     1201 agccgcaaca cttcatcgta caggctgggc gcgcgcagtg cgcgctccaa gtttgcatag
     1261 gtttgcgggt cgccgcgaaa caccttgatg gtatccgcgt tcttgttgcc gatgagaaat
     1321 tccaacagcc gatattgcgg cgattgaaag cccgaggatt tccccaggct gttgcgaaac
     1381 gccgaatagt cggagggagt gatcgtcgcc aatacgtccc aggactgcag caactgctgc
     1441 tggatcgtcg agacgcgcgc cagcatcttg aatgtcgggc cgaggtcgtc gcgctgcacg
     1501 caggcgacgg tcgcgccgag ttcgtgcagg cacagtttca tccatagctc ggagacttga
     1561 tggatgacga tgaacaacat ctcatcgtgc tggtacgaga gcgggtgctg cgcatcgagc
     1621 agcttgtcga gatcgaggta ttgggaatag gtcaacgcat cggtcaaatc ccaatgcacc
     1681 gcctcatcgc tcagatcgac gaacggcttg ggctgcgggg tgggctcctg catcgtcaag
     1741 tcttgaagac ccaaccgggc acggtgcgcc aaccggccag caactcgcgg tccaggcggc
     1801 ggtagtccgg ctcgatcttc tccaccagcc gccagaaact cgccgagtga ttcatatgct
     1861 tggtgtgcgc gagttcgtgc acgaacaaat agcgcacgac gtcgaaatcg agaaacagca
     1921 gcgagcaatt caagctcacg gtaccgcgcg tcgaacagct cccccagcgc gtgcgctggc
     1981 agcgaatcga gacgcgcgcc acctggcaat tgagttcctg cgccagttgc agcaagcgcg
     2041 gcgccaagcg ctcgaaggca gcccgcttca gccagctctg cagcaagaag cgcgcgccgc
     2101 gctcatcggc ggcctgcacc gacagccggc cgggcgattc ctcgagcacg cgtttggcgc
     2161 cgcgccgcca gtcgaccagg aatgtctcgc cgctgcaggc aaaatcgacg cgggtgggaa
     2221 ccggctcgct cgacgccgca aagcactgca tggcggcgac tttgcgatcg atccagggcg
     2281 tgaagcgctg cacgaagtcg cgcaccgtgt gggcattgac gccgagcggc acgacgattt
     2341 ccacgcggcc gccgacgtgc acgcgcgccg tcagccgccg cgcacgcgga ctgacccgaa
     2401 cagcgaacgg atcgctggag ctacgagtct cgaacagcga taactgcgtg ccttgtggct
     2461 tcatcgcgcc ggatcatacc aggtcatggc cgggccaatt gcgccaattg acgttgcgcc
     2521 gccttgaaat cgggcgcagc gatcagcgat ttttcgatgt agttgcgagc tcccacttga
     2581 tcgccgcgcg ccttgagctc gagccccatc gccaggtacg catccgcgtg tccccatccc
     2641 ggcgtatcga ttcgggttgc agagctcgcg tcgaacagct gcacggcgag ctgcaactgc
     2701 gcgaacgcct gctgccgctc ggcgctgccc ggcttcgctg cctggccctg ggagcccgaa
     2761 acatacagcg cccgcggatt gcgcggctcc aagcgcagcg ccgtcttcaa gcgttcgtcg
     2821 gcgcgcgatc gcagcaacac ggcctcgagc ttctcatggt tcgccgcacc ggcataacag
     2881 gcggactgca gtaccagcgc ctcgaccgac ttaacatctt gcgcgaggag cggcttgagt
     2941 tcatcgatgc acgcgccata cgcagattcg gcctcgcgct gcgcgctgcg ctcggccagc
     3001 aagccgcggc gatactgcgc gtgcgccagg tgataatgca gcgccaggtc gccctcatct
     3061 gacttaacct gcgtgctcaa atcgcggatc aagtttcgca ggccgttgtc atcctcggtc
     3121 tggtacgcgt agaggatttg cgcctgcaga tcgccgcctt gctgcgcgct cgccgccgcc
     3181 gcccaacaga gcagcgcgca gagcat
//
LOCUS       DASWZT010000415         3027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_32532, whole genome shotgun sequence.
ACCESSION   DASWZT010000415 DASWZT010000000
VERSION     DASWZT010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32532"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VGE92_11745"
     CDS             <1..496
                     /locus_tag="VGE92_11745"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY1314546.1"
                     /translation="VRGFPYAKITPEAAMNTYPKLSELQSLVGQVLGSSEWLSVDQAR
                     IDQFAAATGDEQWIHVDPVRAAAGPFGTTVAHGFLTLSLIPTLRATAFEVADVRMTVN
                     YGLDRVRFPSPLPAGSRLRGHFKLKSFENIPGGAQLIVEVTAEREGQSKPVCVAESVA
                     RHFI"
     gene            complement(503..1438)
                     /locus_tag="VGE92_11750"
     CDS             complement(503..1438)
                     /locus_tag="VGE92_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEY1314547.1"
                     /translation="MTTAYITHPDCLRHEMGGGHPECPARLTAINEQLRTSGMIEDLR
                     CLEAPLAHGEDLKRVHRAAYVDLIFENAPTEGYVQLDPDTAMNAFSLGAARRAAGAGV
                     LAVDEVTAGRAANAFCAVRPCGHHATQVRSMGFCIFNNIAVAAAYALDKKGLERVAII
                     DFDVHHGNGTEDIFSAPQWQGRVLMASFFQHPFYPYSGTASPAPNMINVPLAEGSDGA
                     AAKRAIETQWLPALDKFKPQMIFVSAGFDAHRDDLLGGMALVEADYEWMTRELMALAA
                     RHSERRIVSMLEGGYNLSALARSVVAHVKTLAEAA"
     gene            complement(1520..2422)
                     /locus_tag="VGE92_11755"
     CDS             complement(1520..2422)
                     /locus_tag="VGE92_11755"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY1314548.1"
                     /translation="MFIRQLEYLVTLAREKHFAKAAEACHVSQPALSSAIRSLEKELG
                     LMIVQRGRRYVGLTSEGERVLTWARQTLASLAHMREDASSAKSSMTGTLRVGAIPTTM
                     TVAAFVTGPCLAAYPNMRFTLSSLTADAIVDQLDRFELDVGLTYLDDTVFGGFEKLHL
                     FDERYVLLADRKVALEPTLSWEQAARLRLCQLSGKMRNRQVIDAAFRRAGVQPAVVLE
                     TDSLFALYAHVSEAGLFSIVPHSLLNFFDLKNRVQARPVLPQLTRAIGLIARNQPALA
                     PLTGALWGIARRLNLQARFDLALA"
     gene            complement(2436..>3027)
                     /locus_tag="VGE92_11760"
     CDS             complement(2436..>3027)
                     /locus_tag="VGE92_11760"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY1314549.1"
                     /translation="AHIRVDQPMGGRILAERVVAEGAEVPFGSNSMRVAEAEFAFRMG
                     RTLEPRDTLYSLPEVMSAVETLHPAIEIPDSRFLDFVGAGAAQLIADNACAHYFVLGS
                     AAPEGWRVLDLAAHVVNARTATLERTGSGAAVLGDPRAALAWLANELSGLGIKLAAGQ
                     VVTTGTCTSPLPIAPGDEVVNDMGILGRVAARFSRD"
BASE COUNT          565 a          978 c          944 g          540 t
ORIGIN      
        1 agttcggggt tttccatatg cgaagataac gccggaggcc gccatgaaca cctacccaaa
       61 attatccgaa ttgcagtcgc tcgttggcca ggttttaggg agcagcgaat ggctcagcgt
      121 cgatcaagct cgcatcgatc aatttgccgc cgcgaccggt gatgagcagt ggattcacgt
      181 cgatccggtg cgggcggcgg ccggaccctt cggcacgacg gtggcgcacg ggttcttgac
      241 gctgagcttg atacccaccc tgcgggccac ggcgttcgag gtcgccgacg tgcgcatgac
      301 cgtcaactat ggcctcgacc gggtgcgctt cccgtcgccg ctacccgcgg gcagccggtt
      361 gcgcggacac ttcaaactca aaagcttcga gaacatcccg ggcggcgcgc agctcatcgt
      421 cgaggtcacc gcggagcgcg agggtcaatc aaaaccggtt tgcgtggcag aatccgtggc
      481 gcgacatttt atttgagcgc ggtcaggccg cctccgccag cgtcttgaca tgtgccacga
      541 cgctgcgggc gagggccgag aggttgtaac cgccctcgag catcgaaacg atacgcctct
      601 cagaatgccg tgccgccaag gccatgagct cgcgggtcat ccactcgtaa tcggcttcga
      661 ccagcgccat gccgcccagc aaatcgtcgc gatgtgcgtc gaatcccgcg gagacgaaaa
      721 tcatttgcgg cttgaactta tccagagccg gcagccattg cgtttcgatg gcgcgttttg
      781 cggcggcgcc gtcgctgcct tcggccagcg gcacattgat catgttgggc gcaggactgg
      841 ccgtgccgct gtacggatag aacgggtgct ggaagaagct cgccatcaag actcgaccct
      901 gccattgcgg cgcactgaat atgtcttcgg tgccgttgcc gtgatgcacg tcgaagtcga
      961 tgatggccac gcgttcgagt cctttcttgt cgagcgcata ggccgccgcc acggcaatat
     1021 tgttgaagat gcaaaaaccc atcgatcgca cctgggtcgc gtggtgcccg caaggtcgaa
     1081 ctgcgcagaa ggcgttcgcc gcgcgtccgg ccgtcacttc atcgaccgcc aacactcctg
     1141 ccccggcggc gcggcgcgcc gcgcccaagc tgaatgcatt catcgccgtg tccgggtcga
     1201 gctgcacgta cccttcggtc ggtgcatttt cgaatatcaa atctacatag gctgcccggt
     1261 ggacgcgttt cagatcctcg ccatgcgcca atggcgcctc caggcagcgc aggtcctcga
     1321 tcatgcccga ggtgcgcagt tgctcgttga tcgcagtcag ccgagcggga cactccgggt
     1381 gcccgccgcc catctcgtgg cgcaggcaat cgggatgcgt gatataggcc gtcgtcattg
     1441 ccgcgcattc tagcgggaac cagcctgcgg cgcagctgcg cgcttgccga tcgagcgccg
     1501 ccacactgcc cgcggcaaat caagccaatg ccagatcgaa ccttgcctgc agattgagcc
     1561 ggcgcgcgat cccccacaac gcgcccgtca ggggcgcaag tgccggctga ttgcgggcaa
     1621 tcaacccgat ggcgcgggtc agctgcggca acaccggccg cgcctgcacc cggttcttca
     1681 ggtcgaagaa gttcagcaag ctgtgcggca cgatgctgaa cagcccggct tcgctgacgt
     1741 gggcgtatag cgcgaacaac gaatcggtct cgaggaccac cgcgggctgt accccggcgc
     1801 gccggaaagc ggcgtcgatc acctgtcggt tgcgcatctt gccgctcaac tggcacagcc
     1861 gcagccgcgc cgcctgctcc cagctcagcg tgggctcgag cgcgactttc cgatctgcga
     1921 gcagcacgta acgctcgtcg aatagatgca gtttctcgaa ccctccgaaa accgtgtcgt
     1981 cgaggtaggt aagaccgaca tcgagttcga aacgatccag ctgatcgacg atggcatcgg
     2041 cggtcaggga cgacagtgtg aaacgcatgt tgggatacgc ggcaagacac ggtccggtca
     2101 cgaacgcggc gacggtcatg gtggtcggga ttgcgcccac cctgagcgtt cccgtcatgc
     2161 tcgatttcgc gctggatgca tcctcgcgca tgtgggccaa cgacgccagg gtctgccgcg
     2221 cccaagtgag gactcgctcg ccttcgctcg tgagccccac atagcgacgt ccacgctgca
     2281 cgatcatgag gccgagttcc ttctccaagc tgcgtatcgc cgatgacaat gccggctgcg
     2341 agacatggca agcttcggcc gccttggcga agtgcttttc gcgggcgaga gtgacgagat
     2401 attcgagctg gcggatgaac atggcggact aaggcttagt cgcggctgaa acgcgccgca
     2461 acgcgtccga gtattcccat gtcgttcacc acctcatccc cgggggcgat cggcagcggc
     2521 gaggtgcacg tgcccgtggt cacgacctga ccggcggcaa gcttgatacc gagccccgac
     2581 aattcattgg cgagccacgc cagcgcggcg cgcggatcgc cgagcacggc cgccccgctg
     2641 cccgtgcgtt cgagcgtcgc cgttctggcg ttgacgacat gcgcggcgag atcgaggacg
     2701 cgccagccct cgggcgcagc gctcccgaga acgaaataat gagcgcacgc attgtcggca
     2761 atcagctgcg cggcgccggc gccgacgaaa tccaggaaac gcgaatccgg aatctcgatc
     2821 gccgggtgca gtgtctccac ggcactcatg acttcgggaa gtgaatacaa cgtgtcgcgg
     2881 ggttccaagg tgcggcccat gcgaaatgca aattctgctt ccgctacccg catcgagttc
     2941 gagccgaaag gcacttctgc accctccgca acgacgcgtt ctgccagaat tcgaccgccc
     3001 atcggttgat cgacgcgaat gtgcgcc
//
LOCUS       DASWZT010000416         2457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_80722, whole genome shotgun sequence.
ACCESSION   DASWZT010000416 DASWZT010000000
VERSION     DASWZT010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80722"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VGE92_11765"
     CDS             <1..250
                     /locus_tag="VGE92_11765"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY1314550.1"
                     /translation="RMRIESARRLIREAGTAASLTDISLAVGIQDASYFARMFRQVVG
                     ESPSAYKRRMSIAGQSADVAQFERSSNDEAFGPAQFTG"
     gene            complement(247..1491)
                     /locus_tag="VGE92_11770"
     CDS             complement(247..1491)
                     /locus_tag="VGE92_11770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY1314551.1"
                     /translation="MNYLFIHQNFPAQYRHVVRDLASRREHRVVFITQPNENSMGDVT
                     KVTYPKDERVSVNCHAYSVEIDRAIYAGASVAEVCRRLRDEGFRPDLIVGHSGWGETL
                     FVKDVFPDVPLLANFEFYYHFQGVDVDFDPEFVSIFNDPARLRTRNATNLLAFEAADW
                     GHSATAWQRSLYPPEMRPRISALHEGVDTDLVRPRRNASFTVPGMQRALTTRDQVVTY
                     VARNLEPYRGFHMFMRALPQVLRRCPRAHVLIVGGDGVSYGAPPPPRSTFRDMMLNEL
                     GAKLDLERVHFLGMIDYQVYLTLLQVSTVHVYLTYPFVLSWSFIEAMASGCLVIGSAT
                     PPVLEVLRDGENGIAVDFFSPRTLANRIEAALGEPGRMQTIRDAARATAVEQFDLKRI
                     LLPRWHALFDDLINHRRPVVLG"
     gene            complement(1512..>2457)
                     /locus_tag="VGE92_11775"
     CDS             complement(1512..>2457)
                     /locus_tag="VGE92_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314552.1"
                     /translation="FIQGPQTAGAWLLAREDYLVTKVHGVGATLVLTSVRGPGGETLS
                     IKIERLDARIDAVAASPAPPPVPEAAPTQAGAAAVAAKNAKSYAKRASSSKAADRKMP
                     SSGTPEAHLPLPVEIGAHIRTRGDLTFTGASWAGRVGTGLWIESFSVKPLQHFEARDI
                     EYKGLTGSGFETPWLSNAAMCGTKGMSVPLLGFAIRLKRSAEAAAFDCEYSGYFKSGV
                     TVGPMRNGAPCRSTVANDPLEGIQVHIRKRSQPIAPAIKSDSETAPPAEAKPERAKVL
                     GPSFGRYRDAGDAEPPAAVAPAPKPAARKAKRSSARRH"
BASE COUNT          438 a          748 c          829 g          442 t
ORIGIN      
        1 gcgcatgcga atcgaatctg cccgtcgtct gattcgcgaa gccggcaccg ccgcctcgct
       61 caccgacatc agtcttgcgg tcggcattca ggatgcatcg tattttgccc gcatgtttcg
      121 ccaagtggtg ggcgagagtc cctcggcgta caagcgacgc atgtcgatag ccgggcaatc
      181 tgcggatgtg gcgcaattcg agcgctccag taacgacgaa gccttcggtc cggcgcagtt
      241 taccggttag ccgagaacca ccggccggcg gtggttgatg agatcgtcga ataacgcgtg
      301 ccagcgcggc agcaggatcc tttttaagtc aaactgctcg acggccgtgg cgcgcgccgc
      361 gtcgcggatt gtctgcatgc ggccaggttc gccgagcgcg gcctcgattc gattcgcgag
      421 ggttcgcgga gaaaagaaat ctaccgcgat gccgttctcg ccgtcgcgca gcacctcgag
      481 caccggcggg gtggcggagc ctattaccag gcagccgctc gccatcgcct cgataaacga
      541 ccaggacagc acgaatggat aggtcaggta tacgtgcacc gtggagacct gcagcagcgt
      601 caagtagacc tggtaatcga tcatgccgag aaagtgcacg cgctccaaat cgagttttgc
      661 gccgagttcg ttcagcatca tgtcgcgaaa cgtgctgcgc ggtggcggcg gcgcgccgta
      721 actgacgcca tcgccgccca cgatcagcac gtgcgcgcgc gggcagcggc gcagcacctg
      781 cggcaacgcg cgcatgaaca tgtgaaagcc gcggtacggc tcgagattgc gtgccacgta
      841 ggtcacgacc tggtcacgcg tggtcagggc gcgttgcatg cccggcacgg tgaatgaggc
      901 attgcgtcgc ggacgaacga gatcggtgtc caccccttcg tgcagcgcgc tgatgcgcgg
      961 ccgcatctcg ggcgggtaca ggctgcgctg ccacgcggtg gcggagtgac cccagtcggc
     1021 ggcttcgaaa gccagaaggt tggtggcgtt gcgggtgcgc aaacgcgccg ggtcattgaa
     1081 gatcgagacg aattcgggat cgaagtctac atcgacgccc tggaagtgat agtaaaactc
     1141 gaagttggcg agcagcggta cgtcgggaaa tacatccttg acgaataacg tctcgcccca
     1201 gccgctgtgg ccgacgatca ggtcgggacg aaatccctca tcgcgcagcc ggcggcacac
     1261 ttcggcgacg ctggcgccgg cgtagatggc gcggtcgatc tcgaccgagt aggcgtgaca
     1321 attgacggaa acgcgttcgt ccttcgggta ggtgaccttg gtcacgtccc ccatggaatt
     1381 ctcgttcggt tgcgtgatga aaacgacgcg atgctcgcgc cggctcgcaa gatcgcgcac
     1441 cacgtgccgg tactgcgccg gaaagttctg gtgaataaac aggtagttca tcgagccgca
     1501 tcgcggccga cttaatgtcg gcgtgcggag gatcgcttgg ctttgcgcgc ggcgggtttc
     1561 ggtgctggcg cgacggcggc cgggggctcg gcgtcgcccg cgtcgcggta gcgtccgaag
     1621 gagggaccga gcaccttggc gcgctcgggc ttcgcctcgg cgggcggggc cgtctcggaa
     1681 tccgacttga tggccggtgc gatcggctgc gagcgtttgc ggatatgaac ctgaatgcct
     1741 tcgagcggat cgttcgcgac cgtcgagcgg cagggagcgc cgttgcgcat tggtcccacg
     1801 gtgacgcccg acttgaagta gccgctgtac tcacagtcga aggcggcggc ttctgcgctg
     1861 cgtttcaagc gaatggcaaa acccaacaag gggaccgaca tgcccttggt gccgcacatc
     1921 gcggcattgc tcaaccacgg cgtttcaaag ccgctgccgg tgagtccttt gtattcaatg
     1981 tcgcgcgcct cgaagtgctg cagcggtttg acggaaaaag attcgatcca gagtcccgtg
     2041 cccacgcgac ccgcccaaga agcgccggta aaggtcaagt cgccgcgcgt gcggatatgc
     2101 gcgccgatct ccacggggag cggcaggtgc gcttcgggtg ttccgctcga cggcatcttg
     2161 cgatccgcgg ctttcgagga cgatgcgcgt ttggcgtagc tcttggcatt ctttgccgcg
     2221 acggcggccg cccccgcctg ggtgggcgcg gcctccggga ccggcggcgg ggccggggag
     2281 gcggcaaccg cgtcgatacg agcatccaag cgctctatct tgatcgacag cgtttcaccg
     2341 ccgggtccgc gcaccgaggt caagaccagc gtcgcgccga cgccgtgtac cttcgtgacc
     2401 aagtaatcct ctctcgccaa aagccacgcg cctgcggtct ggggtccttg gatgaag
//
LOCUS       DASWZT010000417         5550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_22805, whole genome shotgun sequence.
ACCESSION   DASWZT010000417 DASWZT010000000
VERSION     DASWZT010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22805"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..4371)
                     /gene="acnA"
                     /locus_tag="VGE92_11780"
     CDS             complement(139..4371)
                     /gene="acnA"
                     /locus_tag="VGE92_11780"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEY1314553.1"
                     /translation="MTDSFKARTTLQVGAQAFEILSLDALKSHNVARLPFSLKILLEN
                     LLRFEDGVNVTKKDIEALLKWDPTATPSYEIAFTPARVIMQDFTGVPCIVDLAAMREA
                     IVRLGGDPQRVNPLAPAELVIDHSVQVDEYGKADSLQHNNEIEFARNGERYAFLRWGQ
                     TAFSNFKVVPPNTGIVHQVNIERLARVIFSDAAAGKKIAYPDTLVGTDSHTTMVNGLG
                     VLGWGVGGIEAEAAMLGQPVTMLIPQVIGFKLTGALPAGTTATDLVLTVTEILRKHGV
                     VDKFVEFFGDGLKGLPLDDRATIANMSPEFGSTCAIFPIDEETIRYLELTGRAPEQVA
                     LVEAYAKAQGLWRVNGAPAADYTSVVALDLSKVEPSLAGPKRPQDRVPLTNAKSVYRA
                     SVKRMAEERGQKNPKATGSATASVGGKEFPVQDGAVLIAAITSCTNTSNPAVLVAAGL
                     LARNAVARGLSSKPWVKTSLAPGSRVVTDYLTKAGLLKDLEALGFYTVGYGCTTCIAA
                     GTPVLMANGTARTIEQLPEAGGSVLYAPTTERRLARAVQSEMMIQGERECVSLTLQDG
                     RKLVCTPDHRILCADGSYVRADELTLGESRAVAALEAPLDEIGPDEAGYRLPAGERVF
                     SMENETERQCTLAFARLVGHLLSDGSISKQGQGRLNVGQALDREAALCDIELLSGKRP
                     RARAYDERKWSIALPRELTLCVLSLSGVRKGRRIDQAPGLPEFVLDSRCPVAVLREFL
                     GGLFGADGHAPALHCFGKDEQQASIVPPAYSQSAKPEFVAATKRMMNDIIALLVRCGV
                     KARGATVREYAVRRSASTYPASRDGVARREIRLELPEGLSFVERVGFRYCVDKVLRAS
                     AAAVYWRTVDTINRQRLWMADRLEALHGEVQLSFSKTRALAAAELTELETPVFPHYSL
                     LQGHDRFTRLPMADARPFRPLHRDSCEFPSPAELFRELGIRDWFAQPSAHHGQAKRYC
                     TPKDALSLPTLNLKVVDRRAAGKRAVYDLAVNDLHAFVAGTICVHNCIGNSGPLKPEI
                     SEAVRVGDVVACSVLSGNRNFEGRVHPEVKMNFLASPPLVVAYALAGNLDIDITTEAL
                     GNGKDGKPVYLKDIWPSAQEVAAAVGASVDSAMFKKGYANVFSGDANWTAIKTPAGNI
                     YAWDAKSTYVKNPPYFDGMTMTPAPVSDIKHARVLAVLGDSVTTDHISPAGNISKSSP
                     AAKYLLAQGVQAVDFNSYGARRGNHEVMMRGTFANIRLRNLLVPGTEGGVTVHIPSGE
                     QLSIYDAAMKYKAEGTSLVILAGKEYGTGSSRDWAAKGTMLLGVKAVIAESFERIHRS
                     NLIGMGVLPLQFQAGQSAQSLGLSGRETFEIVGLNRGAAKSVKVIADDAGKITEFDAR
                     LRIDTPKELDYYQHGGILQYVLRQLAATSKAA"
     gene            complement(4368..5489)
                     /gene="mnmA"
                     /locus_tag="VGE92_11785"
     CDS             complement(4368..5489)
                     /gene="mnmA"
                     /locus_tag="VGE92_11785"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004646533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEY1314554.1"
                     /translation="MSGGVDSAVAALLLRDAGYTVEGLFMSNWEDDDAYCTSAADYQD
                     ARRVCTVLGIALHRVSFAAQYRDQVFAHFLREYAAGRTPNPDVLCNREIKFGVSLEYM
                     HRLGASWIATGHYARLRRTASGTALLKAADGAKDQSYFLHAVPESALRKTLFPIGELH
                     KADVRRRAHAAGLPVYDKPDSTGICFIGERPFQEFLGRYLRTLPGPIENDHGQILGEH
                     RGLALYTLGQRAGLGIGGRAGSAAAPWYVAAKDMRRNALVVVQAQDHPLLLSDAFDVI
                     DMHWIDSGDAAERQIECAVKTRYRQSDLECRLQGSADRRWHVTLSEPARAVTPGQYAV
                     FYQGARCLGGGVIAERYNRHAAPAGGGIAYNSHFSVEGS"
BASE COUNT         1019 a         1724 c         1753 g         1054 t
ORIGIN      
        1 gccgcgtgtt gagcagtacc gcgggcgcga tcttgcgagc ttcggcgtac gccgccgcct
       61 tgtcgaggat tgccgcgagg ctgccgagcg cacgaataca ggtcggtacg gtggccgtat
      121 acagggtgag aggcatgatt aggccgcctt gcttgttgcg gcgagctgcc gcaatacgta
      181 ttgcagaatc ccgccatgtt ggtagtagtc gagttctttc ggcgtatcga tgcgcagccg
      241 cgcgtcgaac tccgtgatct tgccggcatc gtcggcgatc accttgacgg acttcgccgc
      301 gccgcggttg agtccgacga tttcgaaagt ctcgcggccg ctgagcccga ggctttgcgc
      361 actttggccg gcctggaact gcagcggcag cacccccatg ccgatcaggt tggaacggtg
      421 aatgcgctcg aaactctccg caatcaccgc cttcacgccg agcagcatgg tgcccttggc
      481 agcccagtcg cgcgagctgc cggtgccgta ttccttgccg gcgaggatca ccaaggaagt
      541 gccctcggcc ttgtacttca tcgccgcatc gtaaatcgac agctgctcac cgctgggaat
      601 gtgcacggtg acgccgcctt cggttccggg taccagcaag ttgcgcaacc tgatattcgc
      661 gaaggtgccg cgcatcatca cttcatggtt gccgcggcgc gccccgtagg aattgaagtc
      721 cacggcttgc acgccttgtg ccaataggta cttggccgcc gggctcgact tcgaaatgtt
      781 gcccgcaggt gagatgtgat cggtggtgac cgagtcaccg agcaccgcga gcacccgcgc
      841 atgcttgatg tcgctcaccg gcgccggcgt catggtcatg ccgtcgaagt agggcggatt
      901 tttcacgtac gtggacttgg cgtcccaggc gtagatattg ccggccggcg tcttgatcgc
      961 ggtccagttg gcatcgccgc tgaacacgtt ggcgtagcct ttcttgaaca tggcagaatc
     1021 caccgaggcg ccgacggccg cggcaacctc ctgtgcgctc ggccagatat ccttcaagta
     1081 gaccggcttg ccatccttgc cattgccgag tgcttcggtg gtgatatcga tatcgagatt
     1141 gccggcgagc gcatatgcca ccaccaaggg cggcgaagcc aggaaattca ttttcacttc
     1201 gggatgtacg cggccctcga agttgcgatt gccggacagc accgagcagg ccaccacgtc
     1261 tccaacgcgc acggcttcgg agatctcggg cttcaatggt cctgagttcc caatgcagtt
     1321 atgcacgcaa atcgtcccgg caacaaaagc gtgcagatcg ttgactgcca gatcgtacac
     1381 agcgcgtttg ccggccgcgc ggcggtcgac gaccttgaga ttcagcgtcg gcaagctgag
     1441 cgcgtcttta ggcgtgcagt agcgctttgc ctgaccgtga tgcgccgacg gctgcgcaaa
     1501 ccaatcgcgg atcccgagtt cacggaaaag ctctgccggg gaggggaact cgcagctgtc
     1561 acgatgcagt ggccggaagg gacgcgcatc tgccatgggc agacgcgtga accgatcgtg
     1621 cccttgcaac aaggagtagt gcgggaaaac cggcgtctcg agctcagtca attcggcagc
     1681 ggccagtgcc cgcgtcttgc tgaaggacag ctgcacctcg ccgtgcagcg cttcgaggcg
     1741 gtcagccatc cacaaccgtt gacggttgat ggtatcgacg gtgcgccaat agactgcggc
     1801 cgcactggcg cgaagcacct tgtcgacgca gtagcgaaaa ccaacccgct cgacaaacga
     1861 caggccttca ggcaactcaa ggcggatttc gcgtctcgcc acgccatcgc gtgatgcagg
     1921 atacgtggac gctgagcggc gaacggcata ctcgcgcacg gtagcgcccc gcgctttcac
     1981 gccgcatcgc accagcagcg cgataatatc gttcatcatg cgcttggttg ctgcgacgaa
     2041 ttccggcttg gcgctttgcg aataggcggg tggcacaatg ctcgcctgtt gctcatcttt
     2101 gccaaagcaa tgcagagccg gcgcgtggcc atcggcgcca aaaaggccgc ccagaaattc
     2161 gcgcaaaacg gcaaccgggc atcgactatc caacacgaat tcaggcaaac cgggggcttg
     2221 atcaattcgc ctgcccttgc gcacccctga taaagagagg acgcataggg tgagttcgcg
     2281 cggaagcgcg atagaccatt tgcgctcatc gtaagctctc gcccgcgggc gttttccgct
     2341 caacagttca atatcacaga gcgcggcctc tcggtcgagg gcttgaccga cattcaagcg
     2401 gccctggccc tgtttgctga tcgagccgtc gctcaacaga tggccgacga gacgagcaaa
     2461 ggccaacgtg cattggcgct cggtctcatt ttccatggag aacacacgct cgccggccgg
     2521 cagtcggtaa ccggcttcgt cgggcccgat ctcatcgagc ggcgcttcaa gagcggccac
     2581 tgcgcggctt tcgcccagcg tcagttcgtc cgcgcgcaca taacttccat cggcgcacaa
     2641 gatgcgatgg tcaggcgtgc atacgagctt gcgcccgtcc tgcagcgtga gactgacgca
     2701 ctcacgctcg ccctgaatca tcatctcgct ctgcactgcg cgcgctaggc gacgctcggt
     2761 agtcggtgcg tatagcaccg agccgcctgc ttcaggtagt tgctcgattg tgcgggctgt
     2821 gccatttgcc atcagcacag gagtcccggc ggcgatgcag gtggtacatc cgtagcccac
     2881 ggtataaaag cccagcgctt ccaagtcctt caggagcccg gccttggtca gatagtcggt
     2941 caccactcgc gagccggggg cgagcgaggt tttcacccag ggcttggagg acaggccgcg
     3001 cgcgacggca ttgcgcgcca ggagtccggc cgccacgaga acggcgggat tcgaagtgtt
     3061 ggtgcaactg gtaatggcgg cgatcaacac ggcgccgtcc tgcaccggaa attctttgcc
     3121 gccgacactc gcggtggcgc tgccggtcgc cttgggattt ttctggccgc gttcctcggc
     3181 cattcttttg acgctggctc ggtacacgct cttggcgttg gtcaacggca cgcgatcttg
     3241 cgggcgcttg ggtcccgcca gcgagggttc gactttcgac aggtcgagtg cgaccacgct
     3301 ggtataatcg gcggccggcg caccgttgac gcgccacaac ccctgcgcct tggcgtacgc
     3361 ttcgacgagc gcaacctgtt cgggcgcccg gccggtgagt tcgagatagc gaatggtctc
     3421 ttcatcgatc gggaaaatcg cgcaggtcga gccgaactcg ggcgacatgt tggcgatggt
     3481 ggcgcggtcg tccagcggca gacccttgag gccgtcgccg aagaattcca cgaatttatc
     3541 gaccacgccg tgcttgcgca gtatctcggt cacggtcagc accagatcgg tggcggtcgt
     3601 gcccgcaggc agcgcgccgg tgagcttgaa gccgatcact tgcggaatga gcatggtcac
     3661 cggctggccc agcatggcag cctcggcttc gatgccgccg acgccccagc cgagcacgcc
     3721 caggccgttt accatggtgg tgtgcgaatc ggtgccgacc aaggtgtcgg gatacgcgat
     3781 tttcttgccg gcggcagcgt cggaaaaaat cacccgcgcc agccgctcga tgttgacctg
     3841 atgcacgatg ccggtgtttg gcggcactac cttgaagttg ctgaaggcag tctggcccca
     3901 gcgcaggaac gcatagcgtt cgccgttgcg ggcgaactca atttcgttgt tgtgctgtag
     3961 cgagtcggcc ttgccgtatt cgtcgacttg cacggagtga tcgatgacca gttccgcggg
     4021 cgccaacgga ttgacccgtt gcggatcgcc gccgaggcgc acgatggcct cgcgcatggc
     4081 ggcgagatcg acgatgcagg gtacgccggt gaagtcttgc atgatgacgc gcgccggcgt
     4141 gaaggcgatt tcataactcg gagtggccgt cgggtcccat ttcagcagtg cctcgatgtc
     4201 cttcttggtg acattgacac cgtcttcgaa acgcagcaga ttttccagga gtatcttcag
     4261 cgaaaagggc aggcgtgcga cgttgtggga cttgagtgca tcgaggctca agatctcgaa
     4321 tgcctgcgcg cctacttgca gcgtggttct cgccttgaag ctatccgtca tgacccctcc
     4381 acggaaaaat gagaattata agcgattccg ccgccggcgg gcgccgcgtg cctgttataa
     4441 cgctcggcga tgacaccgcc gccgagacag cgcgcaccct ggtagaagac ggcgtattgc
     4501 cccggagtga ccgcgcgtgc gggctcgctc aaggtgacgt gccagcgccg atcggccgaa
     4561 ccctgcagcc ggcattcaag gtcgctctgc cgataacggg tcttcaccgc gcactcgatc
     4621 tgccgctcgg cggcgtcgcc ggaatcgatc cagtgcatgt cgatgacatc gaaagcatcg
     4681 gacaagagca ggggatgatc ttgcgcctgg accaccacga gcgcattgcg ccgcatgtcc
     4741 ttcgcggcca cgtaccaagg tgcggcggcg ctgccggcgc gaccgccgat ccccaatccc
     4801 gcgcgctggc cgagcgtgta tagggcaagg ccgcggtgct cgcccaatat ctgaccgtgg
     4861 tcgttctcga tcggcccggg aagggtacgc aaatagcggc cgaggaattc ttgaaagggc
     4921 cgttcgccga tgaagcaaat accggtgctg tcgggtttgt cgtacaccgg caggccagcc
     4981 gcatgcgcac gccggcgcac gtcggccttg tgcagctcgc cgatcgggaa caaggtcttg
     5041 cgcaacgccg attccggcac cgcgtgcaga aaatagctct ggtccttggc gccgtccgcg
     5101 gccttgagca gcgcggttcc cgaagccgtg cgccgcaggc gcgcgtagtg gccggtggcg
     5161 atccaggagg caccgagacg gtgcatgtac tcgagcgaca cgccgaactt gatttcccga
     5221 ttgcacaaga catccgggtt cggtgtgcgg ccggcggcgt attcgcgcag aaaatgcgcg
     5281 aatacctgat cgcggtattg ggcggcgaaa ctcacgcgat gcaatgcgat gccgagaacc
     5341 gtgcacaccc gccgtgcatc ttgatagtcg gccgcgctcg tgcaataggc gtcatcgtcc
     5401 tcccaattgg acatgaacaa gccttctacc gtgtagcccg cgtcgcgcag cagcagtgcg
     5461 gccacggctg aatcgacgcc gcccgacatg ccgacgatga ccgtacctgc ggattttgga
     5521 tcggggctct gcacccgcgc agtatagcgg
//
LOCUS       DASWZT010000418         8451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_46908, whole genome shotgun sequence.
ACCESSION   DASWZT010000418 DASWZT010000000
VERSION     DASWZT010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8451
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46908"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /gene="ispH"
                     /locus_tag="VGE92_11790"
                     /gene_synonym="lytB"
     CDS             complement(<1..893)
                     /gene="ispH"
                     /locus_tag="VGE92_11790"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017387043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY1314555.1"
                     /translation="MQIFLANPRGFCAGVDRAIDIVERAVVLFGAPIYVRHEVVHNKY
                     VVDRLRTIGAIFVDELTEVPDGATVIFSAHGVPRVVQQEAKRRMLNVFDATCPLVTKV
                     HMEITRYAREAREVFLIGHAGHPEVEGTIGQFDATLGGAIYLIQTVEDVARVAVRNPE
                     HLAFVTQTTLSVDDTASIVAALRARFPGLKAPVKEDICYATQNRQDAVKDLIGRCEVL
                     VVVGSKTSSNSNRLREMAGKNAKPGYLVDGPEDLHREWFDGVKAVGVTAGASAPEILV
                     QGGIAQLKAWGGAVVDELKGR"
     gene            complement(893..1390)
                     /gene="lspA"
                     /locus_tag="VGE92_11795"
     CDS             complement(893..1390)
                     /gene="lspA"
                     /locus_tag="VGE92_11795"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005037701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEY1314556.1"
                     /translation="MSPEKGRARAAWWLVLSVCVVALDQVSKLYIVGHYTEFEFTRIL
                     PVLDITRMHNVGAAFSFLASASGWQRWVFIGLAVVVSIGITIWLFTLPRGAHRLLAAG
                     LALVLGGALGNLIDRIRLGYVIDFIHFHWDRAYFPAFNVADSAITVGAACLLLDALFE
                     AKRKN"
     gene            complement(1387..4194)
                     /gene="ileS"
                     /locus_tag="VGE92_11800"
     CDS             complement(1387..4194)
                     /gene="ileS"
                     /locus_tag="VGE92_11800"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEY1314557.1"
                     /translation="MTDYKQTINLPNTGFPMKADLANREPALLAVWERNGVYEKIRDV
                     AKGRPSFVLTDGPPYANGAIHLGHAVNKILKDIIVKSRTLDGYDAPYVPGWDCHGLPI
                     EMQVEKTHGRVGQKIDAKAFRAACREYALKQVDAQRGDFKRLGVLGDWDHPYLTMMPR
                     FEAEQLRAFALIIKNGHLYKGYKPVHWCLNCRSALAEAEVEYEDKTSPSVYVRFPVVD
                     TAELAQRFGIDTEVPTSVAIWTTTPWTLPANRAVALHPQFDYALVEVDLGGGVERLIV
                     ASELIDAVMHALGVASWSLIATLKGAALEHMALRHPFYDRLVPVVLGEHVTLDAGTGA
                     VHTAPGHGLDDYVVGRRYGLEVENPVGGDGRFLPGTPLFAGEQVFDANAHVIKVLRDQ
                     SHLLKDEPYHHSYPHCWRHKTPVIFRATPQWFISMEQAGLRKGALEAISHVDWMPAWG
                     EQRISGMIAGRPDWCVSRQRTWGVPIPLFVDKVSGELHPNTAALIEDVAKHVERVGID
                     AWFDLDAAALLGADADRYEKATDVMDVWFDSGVVHHCVSQMRPEITVPADLYLEGSDQ
                     HRGWFHSSLLTSVAMHGRAPYRGVLTHGFTVDEKGRKMSKSIGNTLVPQKLTNTLGAD
                     VVRLWVAATDYANEMSVSDEILKRMADSYRRMRNTLRFLLGNLHGFDPGESALAFDDL
                     VAIDQWAIAKAFALQNDVVTAYRNYEFHAIYQKIHNFCVVELGGFYLDIIKDRLYTTG
                     ASSAPRRSAQTAMHHVAQAMVRWLAPILSFTAEEAWGFLPDARNESVFLNTWHQFPAG
                     AERVSAIDWPALIALKADVARDLERLRTAREIGAPLEAEVTVYAPAAQAGRFAALRDE
                     LRFLLITSQARVVESDTPPNSTVPTSEPGVWIEVKPAMQPKCVRCWHLRSDVGIDPRH
                     PELCARCVLNVEGPGEERLFA"
     gene            complement(4187..5125)
                     /locus_tag="VGE92_11805"
     CDS             complement(4187..5125)
                     /locus_tag="VGE92_11805"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004159.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEY1314558.1"
                     /translation="MELVRGLHNISPCHRGCVLTVGNYDGVHLGHQELIGVLKRRAAE
                     LNSTATVLVFEPNSKEFIDPDGAPPRLSRWREKFLALASYGVERLVTVRFNDCMRAMT
                     PQAFVEELIVEKLGTRHMVVGHDFRYGSKAGGTIESLRVAGQTRGFGVEEVAPFVSDG
                     VRVSSTAVRERLARADYAGAARLLGRPYRMTGRVVHGRRLGRTLGFPTANLRLMRRKS
                     PVSGILAVWAHGIDSRPLAGVASLGTRPTVNGVEPLLEVHVFDFSGDLYGREIEVEFV
                     AKLRDEIKFDSLDAMTEQMQVDSARARDLLAKVDCD"
     gene            5266..5532
                     /gene="rpsT"
                     /locus_tag="VGE92_11810"
     CDS             5266..5532
                     /gene="rpsT"
                     /locus_tag="VGE92_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016138272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY1314559.1"
                     /translation="MANTKSAEKAARQSTVHRARNDAMTSQLRSAIRKATLALKAGKH
                     DEAASALAAATPVIDSMVNKGIIHRNKAARHKSRLTKQVRALAK"
     gene            complement(5548..6585)
                     /gene="obgE"
                     /locus_tag="VGE92_11815"
     CDS             complement(5548..6585)
                     /gene="obgE"
                     /locus_tag="VGE92_11815"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY1314560.1"
                     /translation="MKFVDEAKLKVQAGNGGRGCVSFRREKFVPFGGPDGGDGGLGGS
                     VYLRAVEGMNTLADFRISRTYRGQNGESGSGNDCTGHGGDDIYVSIPVGTVVTDRDTG
                     EQLGDLAAEGQTLLVAKGGKGGWGNTRFKSSTNRTPRKSGLGLPGEKRELALELKLIA
                     DVGLLGLPNAGKSTLIRAVSSARPKVADYPFTTLYPNLGVVSAGPGRSFVMADIPGLI
                     EGAADGAGLGVRFLKHLQRTRVLLHLIDIAPPDPEADPVKDARTIVGELKKFAADLAS
                     KERWLVINKIDLLEPAEAERRAKEIARRLRWKGPVFRISGATGAGTDELKQAVMRYLE
                     EHPRVASLKTA"
     gene            complement(6659..6916)
                     /gene="rpmA"
                     /locus_tag="VGE92_11820"
     CDS             complement(6659..6916)
                     /gene="rpmA"
                     /locus_tag="VGE92_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY1314561.1"
                     /translation="MAHKKAGGSTRNGRDSHSKRLGVKRFGGEDVLAGNILIRQRGTV
                     YRPGVNVGIGTDHTLFAKANGVVQFRVKGVEQHTYVNVVTK"
     gene            complement(6936..7247)
                     /gene="rplU"
                     /locus_tag="VGE92_11825"
     CDS             complement(6936..7247)
                     /gene="rplU"
                     /locus_tag="VGE92_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY1314562.1"
                     /translation="MYAVIATGGKQYRVQEGAVVRIEKLDADQGASVEFTQVLLVGAG
                     SSVTIGAPYVGSAKVVATIESHGKGDKVRIVKFRRRKHYKREKTHRQPYTDVKITQIV
                     A"
     gene            7438..8406
                     /locus_tag="VGE92_11830"
     CDS             7438..8406
                     /locus_tag="VGE92_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019332742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY1314563.1"
                     /translation="MTLEQIRGLVTADFEAVDRVVKQRLHSQVALVDQVATYIIYAGG
                     KRLRPLLVLLAARACGHTEGRHIEAAAIIEFIHTATLLHDDVVDGSSLRRGRETANEV
                     FGNPTSVLVGDFLYSRAFQMMVALDRMRIMEIIADATNAIAEGEVLQLMNAHDPNTTE
                     ARYIDVIRRKTARLFEAGAQIAAVLSDATPAIEQSLALYGRHIGTAFQLVDDALDYQA
                     DEASLGKHLGDDLAEGKPTLPLIYAMQHGNADQRSTIRHAIENGGLDQLAEITRAVAS
                     LGGLAYTARLAQCEADQALAALAPLPETVFKEGLSELARFAVARKN"
BASE COUNT         1736 a         2727 c         2414 g         1574 t
ORIGIN      
        1 tcccggccct tcaactcgtc gaccacggcc ccgccccacg ccttcagctg ggcgatcccg
       61 ccttggacca ggatctcggg cgccgaagca cccgcagtga caccgaccgc tttgacgcca
      121 tcgaaccact cgcgatgcaa atcctcgggt ccgtccacaa ggtaacctgg cttggcgttc
      181 tttcccgcca tctcgcgcag ccgattggaa ttcgaactgg tcttcgaccc gaccaccacc
      241 agcacttcgc agcggccaat caagtccttg accgcgtcct ggcgattttg cgtggcgtag
      301 cagatgtcct ctttcaccgg cgccttcaga cccgggaaac gggcgcgtag cgccgcgaca
      361 atggaggccg tgtcatcgac cgacagggtg gtttgcgtga cgaaggccag atgctcggga
      421 ttgcgaacgg cgacgcgagc cacgtcctcg accgtctgaa tcagatagat tgcgccgccc
      481 agcgtcgcat cgaattggcc gatcgtgccc tccacctccg gatgcccggc gtggccgatc
      541 aagaacactt cgcgcgcctc acgcgcatag cgcgtgattt ccatatgcac cttggtgact
      601 agcggacaag tcgcgtcgaa aacattcagc atgcggcgct tggcttcttg ctgcaccaca
      661 cgcggcacgc catgggcgct gaaaatcacc gtcgcgccat ccggcacttc ggttaattcg
      721 tcgacaaata tggcgcctat ggtgcgcagt cggtccacca catacttgtt gtggaccact
      781 tcatgacgaa catagatcgg ggcaccgaac aacacgaccg cacgctcgac gatgtcgatc
      841 gcacgatcca cgccggcaca aaatccacgc ggattggcta agaaaatttg catcagttct
      901 tccgtttcgc ttcgaataag gcatccaaca acaagcaggc agcaccgacc gtaatcgccg
      961 aatccgccac gttgaatgca ggaaaatacg ctcgatccca atgaaagtgt atgaaatcga
     1021 tcacgtaccc gagccggatg cggtcgatga gattgccgag cgcgccgccg agcaccaaag
     1081 cgaggccggc ggcgagcaac cgatgtgcgc cacgcggcaa cgtgaacagc cagatcgtga
     1141 taccgatgct caccaccaca gcgaggccga tgaataccca acgctgccaa cccgacgccg
     1201 aagcgaggaa gctgaatgcg gcgccgacat tatgcatacg agtgatatcc agaaccggca
     1261 gaatccgggt gaattcgaac tcggtgtaat gcccgacgat atacagcttc gagacttgat
     1321 cgagcgcaac cacacagaca gaaagcacca gccaccacgc cgctcgagca cgacctttct
     1381 ctgggctcat gcgaaaaggc gctcttcacc ggggccctcg acattgagga cgcagcgcgc
     1441 acacagttcc gggtgccgcg gatcgatgcc gacgtcactg cgcagatgcc aacaccggac
     1501 acacttgggc tgcatggcgg gtttgacctc aatccacacg ccaggttcgc tggtcggaac
     1561 cgtggagttc ggcggcgtgt cgctttcgac gactcgtgcc tggctggtga tcaacagaaa
     1621 cctcagctca tcgcgcagcg ccgcaaagcg gccggcctgc gccgcaggcg catacaccgt
     1681 cacctcggcc tcgagaggcg cgccaatttc ccgtgcggtt cgcaagcgtt cgagatcgcg
     1741 cgcgacatcc gccttgagtg cgatcaatgc cggccaatcg atggccgaca cgcgttcggc
     1801 gcccgctgga aattgatgcc aggtattgag gaagaccgat tcgttgcgcg catcgggcaa
     1861 aaaaccccac gcctcctcgg cggtgaaact caagatgggc gcaagccagc gcaccatggc
     1921 ttgcgcaaca tggtgcatgg cggtctgggc cgagcgccgc ggcgcgctgc tcgcgccggt
     1981 ggtgtacagc cggtccttga tgatgtcgag atagaagccg ccgagttcca ccacgcagaa
     2041 gttgtgaatc ttctgataga tggcatgaaa ctcgtagttg cgatacgcgg tcaccacgtc
     2101 gttttgcaac gcgaacgctt tcgcgatcgc ccactgatcg atcgccacca gatcgtcaaa
     2161 cgcaagcgcg gactctcccg ggtcgaagcc gtgaagattc ccgagcaaaa aacgcagcgt
     2221 gttgcgcatg cggcgatatg agtccgccat acgcttcaga atctcatcgg atacgctcat
     2281 ttcgttggcg taatcggtgg ccgcaaccca cagacgcact acatcggcgc ccaaagtatt
     2341 cgtcaatttc tgcggcacca gcgtattgcc gatcgacttc gacatcttgc ggcccttctc
     2401 atccacggtg aatccatggg tcagcactcc gcgatagggc gcacgaccgt gcatggccac
     2461 cgaggtcaag agcgaactat ggaaccagcc gcggtgttga tccgaacctt ccagatacaa
     2521 atccgcgggc acggtgatct ccggccgcat ttgcgatacg cagtgatgca ccacgccgga
     2581 atcgaaccac acgtccatga catcggtggc cttctcgtat cgatccgcat cggcgccgag
     2641 caacgccgcg gcgtccaaat cgaaccaggc gtcgatcccg acgcgctcga catgcttggc
     2701 gacatcttcg atcaaggcgg cggtattcgg gtgcagttcg ccggagactt tgtcgacgaa
     2761 cagagggatg ggtacacccc aggtgcgctg cctcgagaca caccagtccg ggcggccggc
     2821 gatcatgccg ctgatccgct gctcgcccca ggccggcatc caatccacgt gcgaaatcgc
     2881 ctcgagcgcg cccttgcgca agccggcctg ttccatgctg ataaaccact gcggcgtcgc
     2941 gcgaaaaatc accggcgtct tgtggcgcca gcaatgcgga taggaatggt ggtacggctc
     3001 atctttgagg aggtggctct gatcgcgcag caccttgatc acatgcgcat tcgcatcgaa
     3061 tacttgttca cccgcaaata gcggcgtccc gggcaggaat cggccgtcgc cgccgacggg
     3121 attttcgact tccaatccgt agcgtcgccc aacgacgtag tcatccaggc catgtcctgg
     3181 cgctgtatgc acggcgcccg tgccggcatc gagcgtcaca tgttcgccga gcaccacggg
     3241 gaccagccga tcatagaagg ggtggcgcag cgccatgtgc tcgagagccg cgcctttgag
     3301 ggtcgcaatc aagctccaac tcgcgacgcc caaggcatgc atcacggcgt cgatcaattc
     3361 actcgcgaca atcaggcgtt caacgccgcc gcccaaatca acttccacca gcgcgtagtc
     3421 gaactggggg tgtagcgcca ccgctcgatt cgccggcaag gtccacgggg tggtggtcca
     3481 gatggcgacc gaggtcggca cctccgtatc gatcccgaag cgctgcgcca attcggcggt
     3541 atcgaccacc ggaaagcgca catagacgct cggcgaggtt ttgtcctcgt actcgacttc
     3601 cgcctccgcc agtgctgaac ggcaattcaa acaccagtgc accggcttgt aacctttgta
     3661 taaatggcca ttcttgatga tgagtgcgaa tgcgcgcagt tgttcggcct caaagcgcgg
     3721 catcatcgtc aggtaagggt gatcccagtc gccgagcacg cccagccgct tgaagtcgcc
     3781 gcgctgtgca tcgacttgct tgagcgcata ctcgcggcac gccgctcgaa acgctttggc
     3841 gtctatcttc tgtccgacgc gcccatgggt tttttcgacc tgcatctcga tcggcaggcc
     3901 gtgacaatcc cagcccggga catagggcgc atcgtagcca tcgagagtgc gcgacttgac
     3961 gatgatgtcc ttgagaattt tattcacggc gtggcccaag tgaatcgcac cattcgcgta
     4021 cggcggccca tcggtgagaa cgaacgaagg ccgtcctttg gcaacgtcgc gaatcttctc
     4081 gtacacgccg ttgcgttccc acactgcgag cagcgcgggc tcgcggttcg caagatccgc
     4141 cttcatcgga aatcccgtat tcggcaagtt aatcgtttgt ttgtaatcag tcacaatcca
     4201 ccttcgccaa gaggtcgcgc gcgcgtgcgc tatcgacctg catttgttca gtcatcgcgt
     4261 ccaaagaatc gaacttgatc tcatcacgca atttcgcgac gaactcaacc tcgatctccc
     4321 ggccatacaa gtcgcccgaa aaatcgaaca catggacttc gagcagcggc tccaccccat
     4381 tgaccgtcgg ccgcgtgccg agactcgcga cgccggcgag cggacgcgaa tcgatgccat
     4441 gcgcccacac cgccaaaatg cccgacaccg gcgacttgcg acgcatcaaa cgcaggttcg
     4501 cggtcggaaa accgagcgtg cgaccgagcc gccggccgtg caccacgcgg cccgtcatcc
     4561 gatacggtcg accgagcaag cgcgcggcac cggcatagtc cgcccgcgcc aggcgttcgc
     4621 gtaccgccgt gctgctgacg cggacgccgt ccgaaacgaa aggcgcaact tcctccacgc
     4681 cgaatccgcg ggtctgaccc gcaactcgca agctctcgat cgtcccgccg gccttgctgc
     4741 cgtagcgaaa atcgtggccg acgaccatgt ggcgcgtgcc caacttttcg acaatcagct
     4801 cctcgacaaa cgcctgcggg gtcatggcgc gcatgcagtc attgaaacgc acggtcacca
     4861 atcgctccac gccatatgag gcaagggcga ggaacttctc acgccagcgg ctcaggcgcg
     4921 gcggtgcgcc atctggatcg atgaactcct tcgagttcgg ctcaaatacc aacaccgttg
     4981 ccgtcgaatt gagttcagcc gcccgcctct tgagcacgcc gatcaattcc tgatgaccca
     5041 gatgcacgcc atcgtagttg ccgacggtca gaacacagcc tcggtggcac ggcgagatat
     5101 tgtgtagacc gcgaactagt tccatggcga aaggccgtga ttatactcag gtttcccgcg
     5161 catcagtggc gccactatag caagccaagg acaggtcccg aggcctgtaa ttgacaagcg
     5221 cagagccaat cggcaagcta cgcggctctt ttaaggagtc tgcgcttggc gaacacgaaa
     5281 tctgctgaaa aagctgctcg acagtcgacc gtgcatcgcg cccgcaacga cgccatgacc
     5341 tcgcagctcc gcagcgccat ccgtaaggcg actctcgccc tcaaggccgg caagcatgac
     5401 gaagcggcct ccgctctggc cgcggcgacc ccggtgatcg acagcatggt gaacaaaggc
     5461 atcattcacc gtaacaaggc tgcccgtcat aagagccgtt tgaccaaaca ggtccgcgcg
     5521 ctcgccaaat agcctcgcag cgggacttta ggcagtctta agggacgcca cgcgtgggtg
     5581 ttcttccaag tagcgcatca ccgcctgttt gagttcatcg gtgccggccc cggtggcgcc
     5641 cgaaatgcga aacaccgggc ccttccagcg caagcgccgc gcgatctctt tggcacggcg
     5701 ctccgcttca gccggctcca ataggtcgat cttattgatg acgagccaac gttccttgct
     5761 cgcaaggtcg gccgcgaact tcttgagctc gccgacaatg gtgcgcgcat ccttgacggg
     5821 gtccgcctcg ggatcgggcg gtgcaatatc aatgagatgc agcagcaccc gcgtgcgctg
     5881 cagatgtttc aagaagcgaa cacccaaacc cgcaccgtcc gccgcgccct cgatcaagcc
     5941 gggaatgtcc gccatcacaa agcttcgccc gggacctgcg ctgacgacgc cgagattggg
     6001 atacagggtg gtgaacggat agtcggcgac cttggggcga gccgaagaga ctgcccttat
     6061 caaggtcgac ttaccggcgt tgggtaatcc gagcaaaccg acatccgcga tcagcttcaa
     6121 ttcgagcgca agttcgcgtt tctcgcccgg cagcccaaga cctgatttgc gcggtgtgcg
     6181 attggtgctt gatttgaaac gtgtgttgcc ccagccaccc ttgccgcctt tagcgaccaa
     6241 caaggtctgg ccctccgccg cgagatcccc caattgttcg ccggtgtctc gatcggtgac
     6301 gaccgtgccg acgggaatcg acacgtagat atcatcgccg ccgtggccgg tgcagtcatt
     6361 gccgctgccg ctctcaccgt tctgaccccg gtaggttcgg gagatgcgaa agtcagccag
     6421 ggtgttcatt ccctcgaccg ctcgcaggta tacgctgccg ccgaggccac catcgccgcc
     6481 gtcgggtccg ccgaacggga caaacttctc gcgccgaaaa ctgacgcagc cgcggccgcc
     6541 gttaccggct tggaccttga gtttggcttc gtcgacgaac ttcatggtga ctcaaccgga
     6601 ggcgggccgc cctaccccct tcagtcatca tcgcgagctc gatggctcgc aggcatcgct
     6661 acttcgtaac gacgttgacg taggtgtgtt gctcgacgcc tttgacgcga aactggacga
     6721 ccccgttggc cttggcgaac agggtgtgat ccgtgccgat accgacattc acgccggggc
     6781 gatatacggt gccgcgctga cggatcaaaa tgttgccggc gagtacatcc tcaccgccga
     6841 atctcttgac gccgagacgc ttggaatgcg agtcgcgacc attgcgagtg ctaccgccgg
     6901 cttttttgtg tgccatgagc tatctccaga aaaacctagg ctacgatctg cgtgatcttc
     6961 acatcggtgt acggctgccg atgagtcttc tcacgcttgt aatgcttgcg gcggcggaat
     7021 ttgacaatgc gaaccttgtc gcccttgcca tggctctcga tcgtggccac gaccttggcg
     7081 gaacccacat agggagcacc aatggtgacc gacgagccgg cgccgaccag cagcacctgc
     7141 gtgaattcga cgcttgcgcc ttggtctgcg tcgagcttct cgatacgcac cacggcacct
     7201 tcttgaacgc ggtactgctt accgcccgtg gcaatcacgg catacattgt ggaactccga
     7261 gagcggcact actgggccgc aaaagagccg tgaattgtac gggctggccg ccctgaggtc
     7321 aaaccggccg gtttgcgagc cccacggcgc cgtagcacca accggtggcc gggcagccct
     7381 tgtgtcgcgc tagccaccac attcttaggt accatgccgt attcgcttga gcgcttcatg
     7441 acactagaac agattcgcgg cttggtaact gctgattttg aggccgtgga cagggtcgtc
     7501 aaacagcgtt tgcacagcca ggtagcgctc gtcgatcagg tagccaccta catcatctat
     7561 gcgggcggaa agcgcctgcg ccctctattg gtgctattgg ctgcccgcgc ctgcggccat
     7621 accgagggga ggcatatcga ggcggccgcc atcatcgagt tcattcatac cgcaaccctg
     7681 ctccacgatg acgtcgtcga cggctcctcg ctgcgccgcg ggcgcgaaac cgccaacgaa
     7741 gtgtttggca acccgaccag tgtgctggtc ggcgattttc tgtactcacg ggccttccaa
     7801 atgatggtcg cactcgatcg catgcgcatc atggagatca tcgcggacgc gaccaatgcc
     7861 atcgccgagg gcgaggtact gcaactcatg aatgcgcacg acccgaatac caccgaagcg
     7921 cgctacatcg acgtcattcg gcgcaaaacc gcgcgtttat tcgaggccgg cgcacagatc
     7981 gccgcggtgt tgagcgatgc gacaccggcc atcgagcaat ccctggctct gtatggccgg
     8041 cacatcggca ccgccttcca gctggttgac gatgcgctcg actatcaggc tgatgaagcc
     8101 agtctcggaa agcacctcgg cgacgacctg gcggagggca aacccacgct tcccctgatt
     8161 tatgcaatgc agcacggtaa cgccgatcag cgctcgacga tccggcatgc cattgaaaat
     8221 ggcggcctgg atcaactcgc tgaaatcacg cgtgccgttg caagccttgg ggggcttgcg
     8281 tacactgcgc gcctcgctca atgcgaggca gatcaggcgc ttgccgcctt ggcacccctt
     8341 cccgagaccg ttttcaaaga aggtttgagt gaactggcga gattcgccgt ggcgcgcaag
     8401 aactgatgtc gttcgattcg gggtgtagct cagcctggta gagcactacg t
//
LOCUS       DASWZT010000419        18606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_56578, whole genome shotgun sequence.
ACCESSION   DASWZT010000419 DASWZT010000000
VERSION     DASWZT010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 18606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18606
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56578"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VGE92_11835"
     CDS             complement(<1..254)
                     /locus_tag="VGE92_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314564.1"
                     /translation="MRQVISRMRCRHISSTLRAGICHINQEPENTMANYLTVIHECKD
                     YPSWKKAFDADAPNRKAAGLTDIHVLREHGNANLVALMFG"
     gene            complement(291..1763)
                     /locus_tag="VGE92_11840"
     CDS             complement(291..1763)
                     /locus_tag="VGE92_11840"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEY1314565.1"
                     /translation="MPSEFKSPRALVVASVMASMCMIAIEATIISTAMPQIVADLGGL
                     HLYSWVFSAFLLAQTALTVVFGNLADIYGRKPVMLTGIAIFIVGSVFAGFAWSMPTMI
                     LFRLVQGVGAGAMQPVAMTIIADLYPARERGKIQGYLASVWAISAVLGPIVGGFIVRN
                     VSWAWVFWINIPIGLIAAAGFIAFLHEHVKHERRAIDIAGATAFTIAVTSLLIGFTKI
                     GSSDTAALAAAAGVFCVSTTVFLLQERRTANPMIPFALWSHRPIAAANSVAVLSSMAL
                     IGLTTFVPIYVQIVLHRSPVIAGLALTTMLVGWPAGATLGARLFHRFGLHQLLITGAL
                     LQPVGAVLFVLLTPQSSPVIAGVGSLIMGFGMGLLSVSSLVLIQEIVDWSQRGSGTAS
                     NIFSRNLGSTLGATLLGAAFNHGLARATGGAPVTSDRLRQLIEMPPGSSPGDAPLRLA
                     LQHSLNLTFWAMLALTVLTVAIAFMVPSVELRPSSPVGVD"
     gene            1846..2352
                     /locus_tag="VGE92_11845"
     CDS             1846..2352
                     /locus_tag="VGE92_11845"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1314566.1"
                     /translation="MEIRMLTREDAESFRRLRLAALKECPSAFTADYAANLRRPLTHF
                     AAQIQSLPDNFIVGAFHNGTLAGMAGFYRSEGPKLRHKGNIWGMYVAPDLRGSGVGRK
                     LLQEIIAKARSFADIVQVHLSVVADNAKARTLYMSSGFEIVGSEPRAVKVDGKFLDED
                     RLMLRLRE"
     gene            2427..3164
                     /locus_tag="VGE92_11850"
     CDS             2427..3164
                     /locus_tag="VGE92_11850"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY1314567.1"
                     /translation="MKIAAISDIHGNLFALDAVLADIERRGVDHIVNLGDILSGPLLP
                     GETADRLMSLNLPTIRGNHERQVYAMNPAEMGASDRYAFDNITSTHLQWIMALPPVLS
                     FASDVFMCHATPQNDVDCYLEDIVDGELRAAPLNEIEGRSKGCSASLILCGHSHIPRL
                     VRLASGQAIANPGSVGIQAYYGHHPRPHTVEIGSPHARYAIAEQLPLGWGIDFVAVPY
                     DWESAARLAEHRQRADWVRALRSGFVR"
     gene            3275..3757
                     /locus_tag="VGE92_11855"
     CDS             3275..3757
                     /locus_tag="VGE92_11855"
                     /inference="COORDINATES: protein motif:HMM:NF016089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY1314568.1"
                     /translation="MKSLVQWLIEWPRSVAQHLTWLAPLFARITVGWVFMWSGWGKLN
                     NLPQVTENFVGWGIPFANVLSPFVSGVEFFGGLFLLAGLLTRISAGALGITMIVAIRS
                     AKWGDVDSLETLLGFDEFEYLALFLWLAIAGAGAVSVDYLLERWIRVSPSAASAATRQ
                     "
     gene            complement(3720..4139)
                     /locus_tag="VGE92_11860"
     CDS             complement(3720..4139)
                     /locus_tag="VGE92_11860"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY1314569.1"
                     /translation="MRGIDVTKTDRMHTGGCLCGAVRYRVRGALRGVIACHCAQCRRT
                     SGHFAAMSSAPSAAIELTSSAELRWYRSSDTAERGFCGRCGGNLFWRQNGSPTTSITA
                     GSLDTPTQLAIEQHIFVADKSDYYSITDGLPQKLHWD"
     gene            complement(4136..4531)
                     /locus_tag="VGE92_11865"
     CDS             complement(4136..4531)
                     /locus_tag="VGE92_11865"
                     /inference="COORDINATES: protein motif:HMM:NF018430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1203 domain-containing protein"
                     /protein_id="HEY1314570.1"
                     /translation="MESDARRVVATEKPGYPCRVSLADAEVGEELLLLPFAHQNARSP
                     YRSTGPIFVRRAARPRRLAVDEIPDYVALRQISVRAYDAAHMMIDAAVCDGPDTASVI
                     HGIFDNSQAAYIHLHNARRGCFSCRVERA"
     gene            complement(4708..6714)
                     /locus_tag="VGE92_11870"
     CDS             complement(4708..6714)
                     /locus_tag="VGE92_11870"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEY1314571.1"
                     /translation="MRDVKLTPHADLVHSVVAHPLAALPAGLPLTPAVDISWRRCLNE
                     FKLDPAREYRPRVLDQARLKQLQDELEELVQIARAEMDALYEQISGSGYALLLADTQG
                     VILCERVDPTLKKMFLGAGLIVGAEWSERCEGTNGIGTCAAEARPITIHQEDHFRSRH
                     IRLSCSAAPIHDPQGRIVAVLDASCVNAAGSRESQMHTVALVNSSARLIEKCLFLRRH
                     RSDAMLRFHPRAEFVDLLHDGAIAVAANGCIVASDMTGLKLLGAQRPEDLVGRSIAEV
                     FDTTYEELLATSRTGRRAMWELRDNRFRRRYFASLVEAGRQSARSVPSISSPTQLVQI
                     PHDPSPPVLSLADVAGDDPQMMRNLRNAQRVADSAVAILVHGPTGSGKEVFAKAVHMA
                     SQRAQRPFVAVNCAAVPESLIESELFGYVSGAFTGARRDGMRGRILQSSGGTLFLDEI
                     GDMPLSMQTRLLRVLEEHEVTPLGSETLIKVDLRVICASHRDLKEMISLNQFREDLYY
                     RLNGITLELPPLALRRDKEALIRKCLARECDKGATISIENAALQLMFAYSWPGNIREL
                     RNAIRAALAICEERVIRVCDLPGDLHAASALRPLAGETSAAAVSFDRQVSLQSAERDE
                     LLRAIEKNRGRMTTVAAQLGISRNTLYRKIKRHGIQISRRPESH"
     gene            6969..8564
                     /gene="adh"
                     /locus_tag="VGE92_11875"
     CDS             6969..8564
                     /gene="adh"
                     /locus_tag="VGE92_11875"
                     /EC_number="1.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEY1314572.1"
                     /translation="MSPTSSAQARQPSSQPAAGSIHESGAFAAHLKTIERKIALRSQY
                     ENFIGGQWVAPAKGGYFDNISPTTGQVICKIARSQAEDVERAIDAAHAAADKWGRTAV
                     AERARILNRVADRMEEYLPFLAAVETYDNGKPIRETNLADLPLAVDHFRYFASCVRAQ
                     EGSLGELDDDTVAYHYHEPLGVVGQIIPWNFPILMAAWKLAPALAAGNCVVIKPAEQT
                     PMSLLVFAELVQDILPPGVLNIVNGFGLEAGKPLASSPRIAKIAFTGETTTGRLIMQY
                     ASENIIPVTLELGGKSPNIFFADVMREDDAFVDKALEGFAMFALNQGEVCTCPSRALV
                     QKSIYERFMERAVKRVQSIRQGDPYEMSTQIGAQASNDQYEKILSYIDIGRKEGAKVL
                     TGGEANKLPGDLAGGYYIKPTIFEGHNKMRVFQEEIFGPVVSVTVFDTPEQALEIAND
                     TLYGLGSGIWTRDLNTAYRFGRGIKAGRVWTNCYHAYPAHAAFGGYKKSGIGRETHKM
                     MLDHYQQTKNVLVSYSEKALGFF"
     gene            8647..9021
                     /locus_tag="VGE92_11880"
     CDS             8647..9021
                     /locus_tag="VGE92_11880"
                     /inference="COORDINATES: protein motif:HMM:NF017426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HEY1314573.1"
                     /translation="MSTVVAMDPGRVSVTDAARKLIEQLKKTHGPLMFHQSGGCCDGS
                     APMCFQVGEFKLGGVDEKVGEIAGCEFWMDREQFKLWAHTRWTVDVVPGRGASFSLEA
                     PLNMRFLIRSQLCAVPAAKADP"
     gene            complement(9022..11016)
                     /locus_tag="VGE92_11885"
     CDS             complement(9022..11016)
                     /locus_tag="VGE92_11885"
                     /inference="COORDINATES: protein motif:HMM:NF025418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HEY1314574.1"
                     /translation="MLSWTQAWPRVALCCALAASAQIAPAEGVSAYLPLNLEPEMERQ
                     VERVLILADEPILKRPFAVGLVLDALPQACLVDKPLCARVKRYLERYSRDYAVTHASA
                     TGALTHDKDAGVVPNAHGMPVDSSWELSAQGFVQPNDYLLLSGGAIAYQGRTSASGSM
                     LSFGTHWAQLDIGYRDHWFSPATDSSMLISTEAPTMPSVTLSNYEPLTRLGFQYEFFA
                     ARMSRSDRIEYLGQEGSGNPHLFGAQLSIEPFSGWSFGLNRLLQYGGADGLPAGVSFL
                     LRDFFKPSGLSQSQGNQQASYVSRFIFPGKSQFAVYFQYAGEDNSNGGSYLLGNAALT
                     AGIDVPRLARYFDATFEVSEWQNLWYVHNIFLDGMSNDGLVLGNWGADQRNFGDGVGA
                     RSEMLRVGWEPRFGGYLQARIRTLANQGYYGGDLREYSAAIPPFPYHRYLDLTLTYSR
                     PWKDLTVGGEVFGGRDIYGKSFSRLSGFVRYGGDDRTRDDGPLDDDSDSAAPENHAAE
                     RFVDAGFNVNRVRTDLEPGIPITTSKWAFDPHVGLGARRAVSANNDLGVRVELDEVEG
                     HSLIGVRPVDYRYRFTDYLAVGVFWGVARYNLATPAYSEYFGIGALWRNVLPKWDLGF
                     DFRHAQNIARDHVLASDPQGVRPESFYKIDTALLYLSRRF"
     gene            11108..11767
                     /locus_tag="VGE92_11890"
     CDS             11108..11767
                     /locus_tag="VGE92_11890"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEY1314575.1"
                     /translation="MYLPAHFNESRAHVLHPMMRAHPLATLVVACESGLVANHIPVET
                     LSEPAPNGLIRGHIARANPLWRDYPPESEALAIFQGPQAYISPSFYPSKIATGEVVPT
                     WDYAVVHARGTLQFVQDAQWLRALVSRLTNAHEGPRPQPWKIEDAPPPYIEKMLSQIV
                     GFELSIVSLAGKWKLSQNHPQANRLGVAQGLGSAADPQAREVGAMLSALEAERAEPPN
                     E"
     gene            11760..12296
                     /locus_tag="VGE92_11895"
     CDS             11760..12296
                     /locus_tag="VGE92_11895"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1314576.1"
                     /translation="MNSAPAVQGTATPVNVIQTERLVLRQLELHDAEFILELLNQPSF
                     LRFIGDKGVRSLDDARQYIQKGPRDSYDRRGFGLYATCLLDGTPVGICGLVKRDGLED
                     VDLGFAFLSRYFLRGFAVESASAVLAHARQVLGLQRIVAITSPDNVGSIAVLERIGLK
                     FERMIRLQEDSPELKLFG"
     gene            12293..12859
                     /locus_tag="VGE92_11900"
     CDS             12293..12859
                     /locus_tag="VGE92_11900"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY1314577.1"
                     /translation="MNLAVAAVLALIYVLSEAGLAVGRRAKKADSSVQDQGSLGLIWI
                     VISASVTLAFSCAQRYPAAGMGDFAALRIVGIGSFVLGLALRWYSIIYLGRFFTVNVA
                     IASDHRLIDTGPYRFVRHPSYSGALMAFLGLALCLGNWASLVLIVVPVLLVFLRRMQV
                     EEAALLRAFGDRYRDYMRRTKRMIPAIY"
     gene            12876..13313
                     /locus_tag="VGE92_11905"
     CDS             12876..13313
                     /locus_tag="VGE92_11905"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY1314578.1"
                     /translation="MHPFPHRYAVHATVRPDGDVPLSMDGVRIIESAPPKEFDGPGNQ
                     WSPEGLLTAAVADCFALSFRAIAASSKYAWLSLESRTEGTLDRIDGKMRFTRFTTHAK
                     LHVAAGADVERAKRLLEKAENICLVANSLSSERHLTVEIAAGP"
     gene            complement(13310..15190)
                     /gene="rpoD"
                     /locus_tag="VGE92_11910"
     CDS             complement(13310..15190)
                     /gene="rpoD"
                     /locus_tag="VGE92_11910"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEY1314579.1"
                     /translation="MIKKAPAGTATQIADERQSQLKLLIARGKEQGYLTYAEVNDHLP
                     SEIVDPEQIEDIVNMINDMGIPVYEKAPDAESLLLTDATAAPDEEAVEEATAALANLD
                     AEFGRTTDPVRMYMREMGTVELLTREGEIRIAKRIEEGLEAVKIALGSYPGTYDLLLR
                     HYETVKAGQMRLVDVIVGFVDPNAPDEIAQPQNPKMMALEREEAANDDDETEGEEDEE
                     AIDTGPDPEEAGKRFSSIAKIHHQFLNALDKLGSKDPKTLKIRKKICGELMELKLSPK
                     MFDQLIDNLRQHVTEVRLLEKEIMFLCIKQAGMPRKDFISTFPKNETSTRWLDKHIKA
                     KKRYSAPLAKFKDEVERRQNKLKDLEGLFHVLISEIKEINREVSIGEAKARRAKKEMV
                     EANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQA
                     ITRSIADQARTIRIPVHMIETINKLNRISRQMLQEMGREPTPEELAVRMEMPEDKVRK
                     VLKIAKEPISMETPIGDDEDSHLGDFIEDTSVSSPIDSATTESLRETTHSVLAQLTPR
                     EAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEQLRSFLM
                     DD"
     gene            complement(15281..17068)
                     /gene="dnaG"
                     /locus_tag="VGE92_11915"
     CDS             complement(15281..17068)
                     /gene="dnaG"
                     /locus_tag="VGE92_11915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY1314580.1"
                     /translation="MAGRIPQPFIDEVVARSDIVEIINARVPLKKAGREYKSCCPFHS
                     EKSPSFWVSPDKQFYHCFGCGAHGTAIGFLMQYEKMEFFDAVADLAQRAGLELPREAQ
                     APRDPGSADLHEVMARAARFFEQNLADNSRAQQYVAGRGIDAKTSARFGLGYAPDSWD
                     ALLNRFGAQEDERRQIRELGLIIERDTRGGERAAGFYDRFRDRLMFPIRDSRGRVIGF
                     GGRIIDQGEPKYLNSPETPLFHKGRELYGLYEARQARADLKRLLIVEGYMDVVRLHQA
                     GITYAVATLGTATTQEHLNKIFRLTSEVVFCFDGDRAGRQAAWRALENSLPLARDGRE
                     LKFMFLPDGHDPDTLVGAEGSEAFERRLSQALPLSEYLIQQLAAEVDLEHVDGRAKLK
                     SLAAPLFARLPEGIYREMLADGLAARVGMPAATLKRSFASGEAKPAQRREPTAPQRGR
                     MSAGRGNLLTQAITLVLHHPSAAAAVKDPQALRSVDKAGVAVLEELLTQAAAAANPTT
                     ALLLERFRELPEFRRLAELAMAEPLVATAEAAAKELQMAVEKLLEEYGPGRRMDELLR
                     KAEELGLNYDEKAELSELLKFKGRSRLAH"
     gene            complement(17082..17528)
                     /locus_tag="VGE92_11920"
     CDS             complement(17082..17528)
                     /locus_tag="VGE92_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEY1314581.1"
                     /translation="MSLKERITDDMKAAMRSGEKQRLGVIRMITAAIKQREVDERITL
                     DDTQVLSVLEKMIKQRKESLVQFQAGNRQDLVEKEAAEITLLQGYLPSQLSDSELDAL
                     IGEAIAATAAASVKDMGKVMAVIKGRAQGRADMAAVGAKIKAKLGG"
     gene            complement(17546..17803)
                     /gene="rpsU"
                     /locus_tag="VGE92_11925"
     CDS             complement(17546..17803)
                     /gene="rpsU"
                     /locus_tag="VGE92_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEY1314582.1"
                     /translation="MPSVRVRENEYFDAALRRFKRACEKAGVLTELRRREFYEKPTQE
                     RKRKKAAAVKRHLKRLSRENMRGTGGPPSTPPPAAPARPSY"
     gene            17923..>18606
                     /gene="tsaD"
                     /locus_tag="VGE92_11930"
     CDS             17923..>18606
                     /gene="tsaD"
                     /locus_tag="VGE92_11930"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY1314583.1"
                     /translation="MRVIGIESSCDETAVAVFDADEGLLSHRLYSQIAMHQAYGGVVP
                     ELASRDHVRRLLPLVREALADAHSDRQSIDGVAYTAGPGLIGALLVGAGFAASLGYAW
                     GKPVLGIHHLEGHLLAPLLEPNPPRFPFLALLVSGGHTQLVDVAALGEYRILGETLDD
                     AAGEAFDKTAKLLGLPYPGGAALAQLAESGRSGAYVFPRPMLDRPGLDFSFSGLKTAA
                     LVALRGRVLD"
BASE COUNT         3546 a         5648 c         5723 g         3689 t
ORIGIN      
        1 atcccgaaca tgagtgccac cagattggcg ttgccgtgct cgcgcaaaac atggatgtcg
       61 gtgaggccgg ccgcttttcg gttcggcgcg tccgcgtcaa atgccttttt ccaactcggg
      121 taatccttgc actcgtgaat aaccgtcagg tagttggcca tcgtgttctc cggctcttgg
      181 ttgatgtggc aaatccccgc gcgcagggta gatgaaatat gccggcaacg catccgagaa
      241 atgacttggc gcattgcagc ggacgagcgt cactgggaag accgcctgta ctagtccacc
      301 cctaccgggc tcgaaggcct gagttcaacc gacggcacca tgaatgcgat tgcgacggta
      361 agcacagtca gtgcaagcat cgcccagaat gtgagattca gcgagtgttg cagcgccaag
      421 cgcagcggcg catcgccggg tgaggagccc ggcggcattt cgatgagctg gcggagccga
      481 tcggaggtaa ccggtgcacc acccgtcgcg cgggcaagcc catgattgaa tgccgcgccg
      541 agcagcgtgg ctcccaaggt gctcccgagg ttgcgcgaaa atatattcga cgcggttccg
      601 ctgccgcgct gcgaccagtc gacgatttcc tgaatcaaca cgagcgagct gacgctcagc
      661 agacccatcc caaagcccat gatcagagag ccgactccgg caatgacggg agagctctgc
      721 ggcgtcaaca gaacgaacag aaccgcgccg acgggctgca gcagcgcgcc ggtgatcaac
      781 aactggtgca gtccgaagcg gtgaaacaag cgggcgccga gcgtggctcc ggccggccag
      841 cccaccaaca tggtggtcaa cgcgaggcct gcgatcaccg gcgagcgatg caaaacgatt
      901 tgcacgtaga tgggcacgaa agtcgtgagt ccgatgagcg ccatgctgga caacacggcg
      961 acgctattgg cggccgcaat cggccggtga ctccataacg cgaagggaat catcggattg
     1021 gcggtgcgcc gttcttgcag caagaacacc gtggtcgaga cgcagaatac accggccgcc
     1081 gcggcgagtg cggcagtgtc ggaagacccg atcttcgtga acccaatcag caacgaggtg
     1141 accgcgatcg taaaggcggt cgcgcccgcg atatcgattg cgcggcgttc gtgcttgacg
     1201 tgctcgtgca agaaggcgat gaaaccggcg gccgcgatca aaccgatcgg gatgttgatc
     1261 cagaaaaccc aagcccagga gacgttgcgc acgatgaagc cgccgacgat gggtccgagc
     1321 acggcggata ttgcccagac actggccaga taaccttgaa ttttgcctcg ctcgcgagcg
     1381 ggatatagat cggcgatgat ggtcatggcg accggctgca tcgccccggc gcctacgcct
     1441 tgaaccagcc tgaatagaat catcgtcggc atcgaccagg caaagcccgc gaagaccgag
     1501 cccacgatga agattgcgat cccggtcaac atgaccggct tgcggccgta gatatcggca
     1561 agattgccga acaccacggt gagcgcagtc tgcgccagca ggaaggccga aaatacccaa
     1621 ctataaaggt gcaacccgcc caaatccgcg acgatctgcg gcatggcggt cgatatgatg
     1681 gtcgcctcga ttgcaatcat gcacatggat gccatcacgg acgcgacgac aagcgctcgc
     1741 ggggatttga attccgacgg caactagcga atctccgagc aggcgcccgc agcgccaatg
     1801 tgccagtcta tcattgcgtg ccggacgctg atcgcaagga gttccatgga aatacgcatg
     1861 ctcacccgcg aggacgccga gtccttccgc cggctgcggc tcgcggcgct gaaagagtgt
     1921 ccgtccgcgt tcaccgcgga ctatgcggcg aatctgcgac gaccgctgac tcatttcgcc
     1981 gcgcagattc aatcgctgcc ggataatttc atcgttggcg cttttcacaa cggcacgctc
     2041 gcgggcatgg cgggctttta tcgaagcgaa ggacccaagc ttcggcacaa gggaaatatc
     2101 tggggtatgt atgtcgcccc ggatctgcgc ggcagcggcg tcgggcgtaa attactgcag
     2161 gaaatcatag ccaaggcgcg cagcttcgcg gatatcgtgc aggtacattt gtcggtggtt
     2221 gccgataacg cgaaagcgcg aactctgtat atgagcagcg gctttgaaat cgtaggcagc
     2281 gagccgcgcg cggtcaaagt cgatggaaag ttcctggatg aggatcgact gatgctgcgg
     2341 ctgagggagt aattgccggc gagaaagtgc aacgccggcg tcgagattcc cgactataat
     2401 cacgagattc aaccgcccgg atcccgatga aaatcgccgc catctccgac atccatggca
     2461 atttgttcgc cctcgacgcg gtgttggccg acatcgagcg ccgcggcgtc gatcacatcg
     2521 tcaacctggg cgacattctc tccggcccgc tgctgccggg cgagaccgcc gatcgattga
     2581 tgtcgctcaa tctgcccacg attcgcggca accacgagcg acaagtgtac gcaatgaacc
     2641 ccgccgaaat gggcgcctcg gatcgctacg cgttcgacaa tatcaccagc acccacctgc
     2701 agtggatcat ggccctgcca ccggtgctga gtttcgcgag cgatgttttt atgtgccatg
     2761 cgacaccgca aaacgatgtc gactgttatc tagaagacat tgtcgacggc gagcttcgcg
     2821 ccgcgccgtt gaacgaaatc gaagggcgtt ccaagggctg cagcgcttcc ttgattctct
     2881 gcggccactc gcacattcca cgcctggtgc gcctcgcgag cggccaggcc atcgccaacc
     2941 ccggaagtgt cggaattcaa gcctactacg gccatcatcc gcgtcctcac accgtggaga
     3001 tcggctcgcc gcatgcgcgt tatgcgatcg cagagcaatt gccgttggga tggggcatcg
     3061 attttgtcgc ggtcccttat gactgggagt ccgccgcgcg cctcgccgag cacaggcaaa
     3121 gagcggattg ggtgcgcgca ctgcgcagcg ggttcgtgcg ctaggcgcca agatcgcggc
     3181 cgcgctgcaa gcgctgaaat aaaccccggt tcctgccggt ctaacactat gatcgacacg
     3241 tacagtttca aaaaactcag ggagcgcccc gcccatgaaa tctctcgtcc agtggctgat
     3301 tgaatggccg cgaagcgttg cgcagcattt gacctggttg gcgccgctgt tcgcgcgcat
     3361 tacggtcggc tgggtgttca tgtggagcgg ctggggcaag ctcaacaatc tgccgcaggt
     3421 caccgagaat ttcgtcggct ggggcatacc ctttgccaat gtattgagcc cgtttgtatc
     3481 cggagtcgag ttcttcggcg ggctgtttct gctcgcgggg ttgctcactc gaatatccgc
     3541 cggagcattg ggaatcacca tgatcgtcgc catccgctcc gccaaatggg gcgacgtcga
     3601 ctcgctggaa accttgctcg gattcgacga gttcgaatac ctcgcactgt tcctgtggct
     3661 cgcgatcgcg ggcgccggcg cggtttctgt cgactacttg ctggaacgtt ggattcgagt
     3721 cagtcccagt gcagcttctg cggcaacccg tcagtgatgc tgtaatagtc cgacttgtcg
     3781 gcgacgaaga tgtgctgctc gatcgcgagt tgagttggcg tgtcgagtga gccggcagta
     3841 atgctggtgg tggggctgcc gttctgccgc caaaacagat tgccgccgca gcggccgcag
     3901 aacccgcgct ccgcggtgtc cgatgacctg taccagcgca gttccgcgga actcgtgagc
     3961 tcgatagctg ccgacggcgc gctggacatc gccgcgaagt gcccgctggt tcgacggcac
     4021 tgggcgcaat gacaggcgat gacgccgcgc agggcaccgc gcacgcgata gcggacagct
     4081 ccgcacagac agccgccggt gtgcatcctg tcggttttgg tcacatcgat tcccctcaag
     4141 cccgctctac ccggcacgaa aagcatccgc gccgcgcgtt gtgcaaatgg atgtaggcgg
     4201 cctgcgaatt gtcgaaaatt ccatgaatga ccgatgcggt gtccgggccg tcgcaaaccg
     4261 ccgcatcgat catcatgtgc gccgcgtcgt aggcgcgaac ggagatttga cgcagagcca
     4321 catagtccgg gatctcatcg acggcgagcc ggcgcgggcg cgccgcgcgg cgcacgaaaa
     4381 ttggtcccgt ggaccgatac ggtgagcgcg cattctggtg ggcaaacggc agcagcagga
     4441 gctcctcgcc cacctcggcg tcggcgaggc tgacgcggca cgggtagccg ggcttctcgg
     4501 tggcaaccac gcgccgcgcg tccgattcca tcagctctgg atcagtgagg gagaaaagtg
     4561 cggcaaatgg ctcaaaagac aatcccgcca attggaatga catcgcgcag ctccgatcgt
     4621 gaaaccctgg gagcgcatat tgctgcaatt tgggcggcct gtctggccgt tttcggactt
     4681 cgatttctgg ccgaaagcag cgccgaatca atggctttcg ggccggcggc tgatttgaat
     4741 gccatgccgc ttgatcttgc gatacaaggt gtttctgctg atgccgagtt gtgccgccac
     4801 ggtggtcatg cgacctcgat tcttctctat cgcccgcagc agctcgtcgc gttccgccga
     4861 ttgcagcgag acttggcggt cgaacgatac ggcagctgcg gacgtctcac ccgccaacgg
     4921 acgcaaggct gacgccgcgt gcaagtcccc gggcagatcg caaacgcgga tgactcgctc
     4981 ttcgcaaatt gccagcgccg cgcgaatcgc gttgcgtaat tcgcgaatat tgccgggcca
     5041 gctgtaggcg aacatgagtt gcagcgcggc attttcgatg gaaatggtcg cgcctttgtc
     5101 gcactcgcgc gcgaggcatt tgcggatcaa agcttccttg tcgcggcgca atgccagcgg
     5161 cggcaactcc agcgtaatgc cgttcagccg atagtagaga tcttcccgaa actgattgag
     5221 agagatcatc tccttcaggt cgcgatgcga cgcgcaaatg acgcgcaaat caaccttgat
     5281 cagcgtctcg ctgccgagcg gcgtcacttc gtgctcctcg agcacgcgca gcaggcgagt
     5341 ttgcatggac aagggcatgt cgccgatctc gtccagaaac aaggtaccgc ccgatgattg
     5401 caggattcga ccgcgcatcc cgtcgcggcg cgcgccggta aatgcgcccg atacgtagcc
     5461 gaatagttcg ctctcgatca gcgactcagg cacggctgcg cagttgacgg cgacgaatgg
     5521 gcgctgtgca cgctggctcg ccatatgcac ggccttcgcg aacacctctt tacccgatcc
     5581 cgttggaccg tgaaccaaga tggccaccgc agagtccgcc acgcgctggg cattgcgcaa
     5641 gttgcgcatc atctgcgggt catcgccggc gacatccgcc aagctgagaa ccggcggcga
     5701 cggatcgtga ggtatctgaa ccagctgagt gggcgaggaa atcgatggca cggagcgcgc
     5761 gctctgccgc ccggcctcga ccagactcgc aaagtagcgt cgccggaagc gattgtcgcg
     5821 cagttcccac atggcccggc gtccggtacg cgaagtcgcg agcagctcct cgtacgtggt
     5881 atcgaagacc tcggcgatcg atcgccccac cagatcctcg ggccgctgcg cgccgagcaa
     5941 tttgaggccc gtcatgtcac ttgcgacgat gcagccgttg gctgccaccg caattgcgcc
     6001 atcgtgcagc aaatcgacga attcagcccg cgggtgaaat cgcaacatgg cgtcagagcg
     6061 gtgccgcctc aggaacagac atttctcgat caggcgtgcc gaggaattga cgagcgccac
     6121 cgtgtgcatt tgggattcac ggctgccggc ggcgttgacg caggacgcat ccaacacggc
     6181 gacgatgcgt ccctgcggat cgtggatcgg agcggccgaa cacgacagac ggatatgacg
     6241 cgagcggaag tgatcttcct gatgaatggt gatgggccgt gcttcggcgg cgcacgtgcc
     6301 tattccgttg gtcccctcgc aacgctcgct ccactctgcc ccgactatca gcccggcgcc
     6361 gagaaacatc ttcttcaggg tcgggtcgac cctctcgcag aggatgacac cttgagtatc
     6421 cgcgagcagc agtgcgtagc cggagccgga aatctgctcg taaagcgcgt ccatctccgc
     6481 gcgagcgatt tgcaccagct cttcgagctc gtcctgcaat tgtttgagcc gcgcttggtc
     6541 gagcactctg ggccgatact cccgcgccgg atcgagcttg aattcgttca agcagcgccg
     6601 ccaggatatg tccactgccg gcgtcagcgg caagcctgct ggcagtgcag caagcggatg
     6661 cgcgacgacg ctgtgcacga gatccgcatg gggagtcaat ttgacgtccc tcatgtgatg
     6721 cctgccttga tcgcgattct tatcagtgtc gtagccacgt ccagccccga agcgccccca
     6781 tgcctttaaa ccgctatccc aagatagtgt caatagcggg ttgacaactg tcacgagaat
     6841 gacacgaagt acccgcgaaa agcctgaacc cgcgggtccc gacgctgtta ccctctggca
     6901 cgctgcttgc tcccctccgt atgtcgaagg caagcctgcc cgtcgccgtt acctaaagga
     6961 gatcgttcat gagtcccacg agttccgcgc aagcgcggca gccgagcagt cagccggccg
     7021 ccggctccat ccacgaaagc ggcgcattcg ccgcgcatct gaaaacaatc gaacgcaaga
     7081 tcgccttgcg ctcgcaatat gagaatttca tcggcggcca atgggtggcg ccggcaaagg
     7141 gaggctattt cgacaacatc tcgccgacca cgggccaggt catttgcaag atcgcccgct
     7201 cgcaggcgga ggatgtcgaa cgcgcgatcg acgctgccca cgcagccgcc gacaaatggg
     7261 ggcggaccgc ggttgctgaa agagcccgca tcctcaatcg agtggcggat cggatggaag
     7321 aatatctgcc gtttctcgcg gccgtcgaaa cctacgacaa cggcaagccg attcgcgaaa
     7381 cgaatctcgc ggacctgccg ctggcggtgg atcacttccg ctacttcgca agctgtgtgc
     7441 gcgcccagga aggatcgctg ggcgaactcg acgatgatac cgtggcatac cattatcacg
     7501 agccgctcgg cgtcgtcggc caaatcattc cgtggaattt cccgatattg atggcggcat
     7561 ggaagcttgc cccggcgctc gccgcgggca attgcgtggt catcaaaccc gccgaacaga
     7621 caccgatgtc gttgctggtc tttgccgagc tcgtgcaaga catcctgccg ccgggcgtgc
     7681 tcaacatcgt caatggcttc ggcctcgagg ccggcaagcc gctcgcatcg agtccgcgta
     7741 tcgccaagat tgcgttcacc ggagagacca cgacggggcg cctgatcatg cagtacgcct
     7801 ccgagaacat cattccggtc acgctcgaac tcggcggcaa gtcgccgaac attttcttcg
     7861 cggacgtgat gcgcgaggac gacgcgtttg tggacaaggc tttggagggt ttcgcgatgt
     7921 ttgccctcaa tcaaggtgag gtctgcacct gcccgtcgcg ggccttggta cagaaatcga
     7981 tctacgagcg cttcatggaa cgcgccgtga aaagagtgca gagcatccgc caaggcgatc
     8041 cgtatgagat gagcacgcaa atcggcgcgc aggcctcgaa cgaccagtac gagaagatat
     8101 tgtcgtatat cgacatcggc aggaaggaag gcgccaaagt tctcaccggc ggcgaggcca
     8161 acaaattgcc gggtgacctc gccggcgggt attacatcaa gccgacgatt ttcgaggggc
     8221 acaacaagat gcgcgtgttc caagaggaga tcttcgggcc cgtggtgtcg gtcacggttt
     8281 tcgatacgcc ggagcaggcc ctcgagatcg ccaacgacac cctgtatggg ctgggttccg
     8341 gcatctggac ccgcgatctt aacaccgcct atcgattcgg ccggggcatc aaggcgggcc
     8401 gcgtttggac caattgctac catgcgtacc ccgcgcacgc ggcgttcggc ggctacaaga
     8461 aatccggcat cgggcgcgag acgcacaaga tgatgctgga tcactatcag cagacgaaga
     8521 acgtgctggt gagctacagc gaaaaggcgt taggcttctt ctagcgccgg cgtcggcgcg
     8581 tgcagcggcg ggcggatctg cttgcggatc cgcccgcatt gtgaacttaa gatcgaggag
     8641 ttgctcttga gcacggtcgt cgcgatggat ccaggccgcg tcagcgtcac cgacgcggct
     8701 cgcaagttga tcgagcagct gaagaaaact cacggcccgc tgatgtttca ccagtcgggc
     8761 ggctgttgtg acggcagcgc accgatgtgc tttcaagtcg gcgagttcaa attgggcgga
     8821 gtcgatgaga aagtcgggga gattgccggc tgcgaattct ggatggaccg cgagcagttc
     8881 aaactctggg cccatacgcg ttggacggtc gatgtcgtcc ccggccgggg cgccagtttt
     8941 tcgctcgaag cgcctctcaa catgcgcttc ctgattcgct cgcagctgtg cgccgtgccg
     9001 gcagccaaag ccgatccctg atcagaagcg gcgcgacaag tacaggagtg cggtgtcgat
     9061 cttgtagaag ctttcgggcc gcacgccttg cggatcgctt gccaacacat gatcgcgcgc
     9121 gatgttttgc gcatggcgga agtcgaatcc taaatcccat ttcggcaaga cgttgcgcca
     9181 cagggcgcca atgccgaagt attcggaata ggcgggcgtc gcgaggttgt agcgcgccac
     9241 gccccagaaa acaccgaccg cgagataatc ggtgaagcgg tagcgatagt cgacgggtcg
     9301 cacgccgatg agcgaatgac cctcgacttc gtccaattcg acccgcacgc ccaaatcgtt
     9361 gttggcagac acggcgcggc gggcgcccag cccgacgtgg gggtcgaagg cccatttgct
     9421 cgtggtgatg gggatgcccg gctccaaatc tgtgcgcacc cgattgacgt tgaatccggc
     9481 atcgacgaat cgttctgcag cgtggttctc gggagcggcg ctgtcggaat cgtcgtcgag
     9541 cggcccgtca tcccgagttc gatcatcgcc gccataacgc acaaatcccg acaatcgcga
     9601 gaacgacttg ccgtagatgt cgcggccgcc gaatacctcg ccgccgacgg tcaggtcctt
     9661 ccaaggccgc gagtaggtca gcgtcagatc caggtagcga tggtagggaa aggggggaat
     9721 cgccgcagaa tattcgcgca gatcgccgcc gtagtaacct tggttcgcga gcgtgcgtat
     9781 ccgtgcctgc agatagccgc cgaagcgcgg ctcccaaccg acacgcaaca tttcgctgcg
     9841 cgcgccgacg ccgtcaccga agttgcgctg gtcggctccc cagttgccga ggacgagccc
     9901 atcgttgctc attccgtcca ggaaaatatt gtgtacatac cagaggtttt gccactcgga
     9961 aacctcgaat gtcgcatcga agtagcgtgc gagccgcgga acgtcgatgc cggcggtgag
    10021 cgccgcgttg ccgagcaagt agctgccgcc gttggagttg tcctcaccgg cgtattggaa
    10081 atacacggcg aattggcttt tacccggaaa gatgaagcgg ctgacatagg acgcttgttg
    10141 gttgccctgg ctttgcgaca gcccgctcgg cttgaaaaaa tcccgcaaca aaaaactcac
    10201 gccagccggc aagccgtcgg cgccgccgta ttgaagcagc ctattgagac cgaacgacca
    10261 gccgctgaag ggctcgatgg aaagctgcgc tccgaacaag tgcgggttgc cgctgccctc
    10321 ctggcccagg tactcgatgc ggtcggagcg cgacatgcgc gccgcgaaga attcatattg
    10381 aaaaccgagc cgggtgagcg gttcatagtt cgacagggtt accgacggca tggtcggcgc
    10441 ctcggtgctg atcagcatgc tgctgtcggt ggcgggagag aaccagtggt cgcggtagcc
    10501 tatgtcgagt tgcgcccaat gggtgccgaa actcaacatc gagccgctgg ccgaggtgcg
    10561 gccttggtag gcgattgcgc cgccgctcaa caggaggtag tcgttcggtt gcacgaagcc
    10621 ctgtgccgat aactcccagg agctatccac cggcataccg tgagcattgg gcacgacgcc
    10681 tgcgtcttta tcgtgcgtaa gcgcaccggt ggcactcgcg tgggtgactg catagtcgcg
    10741 tgaatagcgc tccagatatc gcttgaccct ggcgcacaac ggtttgtcga ccaggcaggc
    10801 ttgcggcaag gcgtcaagca ccaggcccac tgcaaacggc cgcttgagaa tgggttcgtc
    10861 ggcgagaatc agcactcgtt cgacttggcg ttccatctcc ggttcaagat tgagcggcag
    10921 gtaggcgctc acaccctctg caggcgcgat ctgcgccgac gcggccagcg cgcagcacag
    10981 tgcgactcgc ggccatgcct gggtccacga caacacgata ttcaattgac tccgccgccc
    11041 gttaaactat cgtggcagta cacgatagga cgcgtgagac ggtcaaggct tttatgaggg
    11101 attgatattg tacctgcctg cacatttcaa cgaatcccgc gcccacgtgc tgcacccgat
    11161 gatgcgcgcg cacccgttgg cgacgctggt cgtggcatgc gaatcagggc tggtcgcaaa
    11221 ccacatccca gtcgaaactc tgtccgagcc ggcaccgaac ggtttgatcc gaggccacat
    11281 cgcgcgcgcg aatccgttgt ggcgtgacta cccgcccgag tccgaagcgc tcgccatctt
    11341 tcaggggccg caagcctata tttcgccgtc cttttatccg tcgaagattg cgaccggcga
    11401 agtcgtgccg acgtgggatt atgcggtcgt gcatgctcgc ggcaccttgc aattcgtaca
    11461 ggatgcgcag tggctgcgag cactggtgtc gcgtttgacg aacgcgcacg aggggccgcg
    11521 gccgcagccg tggaagatcg aggacgcacc gccgccctat atcgaaaaaa tgctgtctca
    11581 gatcgtcggc ttcgaattgt ccattgtcag tttggccggc aaatggaagc tcagccagaa
    11641 tcacccgcaa gcaaaccggc tgggcgtggc acagggtctc ggcagcgccg ccgacccaca
    11701 ggcgcgcgaa gtgggcgcaa tgctcagcgc cttggaggcc gagcgcgccg agccgccgaa
    11761 tgaatagcgc gcccgcagtg cagggcacgg ccaccccggt gaacgtcatc cagaccgaac
    11821 ggctggtgct gcgccagctc gagctgcacg acgccgagtt cattttggag ctgctcaatc
    11881 agccgtcatt tctgcgtttc atcggcgaca agggcgtgag aagcctcgac gacgcgcgtc
    11941 aatacatcca gaagggaccg cgtgacagtt atgaccggcg cggcttcgga ctgtatgcga
    12001 cgtgcttgct cgacggcacg ccggtgggga tttgcggtct ggtcaaacgc gacggcctcg
    12061 aggacgttga cctggggttt gcgttcttgt cgagatattt tctgcgggga ttcgccgtcg
    12121 aatccgcgtc ggctgtgctg gcgcatgcgc ggcaagtgct gggattgcag cgtatcgtcg
    12181 ccattacctc gcccgacaac gtgggttcca tcgcggtact cgagcgaatc ggtctgaagt
    12241 tcgaacgcat gatcagactt caagaagaca gtcccgaact taagttattc ggatgaatct
    12301 cgcggtcgcg gcggtgctcg cactgatcta tgtgctgtcg gaggcggggc tggctgtcgg
    12361 taggcgcgcc aagaaggccg attcgagcgt ccaagaccag ggctccttgg gcttgatatg
    12421 gatcgtgatt tccgcgagcg tcaccctggc cttcagctgc gcgcagcgct atccggcggc
    12481 cggcatgggg gactttgcgg cactgcgcat agtgggaatc ggctcattcg tgctcggatt
    12541 ggcgctgcgc tggtattcga tcatttacct gggacgtttc tttacggtca acgtcgccat
    12601 cgcgtcggac caccgtctca tcgacaccgg accgtatcgg tttgtgcggc atccttccta
    12661 ctcaggtgct ttaatggcgt tcctcggact agcgctgtgc ttgggcaact gggcatcgct
    12721 tgtgctgata gtcgtgcccg tcctcctggt attcctgcgg cgcatgcaag tcgaggaagc
    12781 cgcgctgctg cgggcttttg gcgatcggta ccgggattac atgaggcgca ccaagcgcat
    12841 gattccggcc atttattgaa tcctcggagt agccaatgca cccctttcct catcgctatg
    12901 ccgtccatgc cacggtgcgc ccggacggcg atgtaccgct atcgatggac ggtgtcagga
    12961 tcatcgaatc ggcgccgccc aaggagttcg acggtccggg caatcaatgg tcgcccgagg
    13021 ggctcttgac cgcagccgtc gccgattgct tcgccttgag ttttcgggca attgcggcgt
    13081 cgtcgaagta tgcgtggctg agcctcgagt cgcgcaccga aggcacgctg gaccgtatcg
    13141 atggcaagat gcgcttcacg cgctttacca cccacgccaa attacatgtt gccgcgggtg
    13201 cggatgtcga gcgtgccaag agactgctcg agaaggcgga gaatatctgc ctggtcgcga
    13261 attcactcag tagtgagcgg catctgacgg tggaaatcgc cgccggacct tagtcgtcca
    13321 tcaagaagct gcgcagctgc tcgctgcggc tggggtgacg caactttcgc agcgccttgg
    13381 cctcgatctg ccgtatgcgt tcgcgggtta cgtcgaactg cttacccact tcctcgagcg
    13441 tgtgatccgt gttcatgtcg atgccgaagc gcatccgcag aacctttgct tcgcgcggcg
    13501 tcaactgcgc caaaaccgag tgcgtggttt cacgcagcga ttcggtggtg gccgagtcga
    13561 tcggcgagga cacggacgta tcttcgatga aatcgcccaa gtgcgaatcc tcatcatcgc
    13621 cgatcggcgt ctccatggaa atcggctctt tggcgatttt caggactttg cgcaccttgt
    13681 cttccggcat ctccatgcgc accgccaatt cttccggcgt gggttcacgg cccatctctt
    13741 gcagcatctg gcgcgagatg cgattcagct tattgatggt ttcgatcatg tgcaccggaa
    13801 tgcgaatggt gcgcgcctgg tcggcaatgg agcgcgtgat ggcctgacgg atccaccatg
    13861 tcgcataggt cgagaatttg taaccgcggc gatattcgaa tttgtcgaca gccttcatca
    13921 aaccgatgtt gccttcctgg atcagatcca ggaactgcag gccgcggttg gtgtatttct
    13981 tggcgatcga aatcaccagg cgcaggttgg cctcaaccat ttccttcttc gcgcgccgcg
    14041 ccttggcctc gccgatcgat acttcgcggt tgatctcctt gatctcgctg atgagcacat
    14101 gaaacaagcc ttcgagatcc ttcaacttgt tctggcggcg ctcgacttcg tccttgaatt
    14161 tggcaagcgg cgccgagtag cgcttcttgg ccttgatatg cttgtcgagc cagcgcgtac
    14221 tggtctcgtt cttcggaaag gtcgagataa agtccttgcg cggcatgccc gcctgcttga
    14281 tgcaaaggaa catgatctct ttctcgagaa gtcgcacctc ggtcacgtgc tggcgcagat
    14341 tgtcgatcag ttggtcgaac atcttcggcg agagcttcaa ttccatcagc tcgccgcaga
    14401 tcttcttgcg gatcttcaaa gtcttggggt ctttcgatcc cagcttgtcg agtgcgttga
    14461 ggaactgatg gtgaatcttc gcgatcgagc tgaatcgctt gccggcctct tccgggtccg
    14521 gacccgtatc gatggcctct tcgtcttctt caccttcggt ctcgtcgtcg tcgttggcgg
    14581 cttcttcgcg ttccaacgcc atcattttcg gattctgcgg ctgcgcgatt tcgtccggcg
    14641 cattcggatc gacgaatccg acgatgacat cgaccaggcg catctgcccg gccttgacgg
    14701 tctcgtagtg ccgcagcagt aaatcgtaag tacccgggta gctgccgagc gcgatcttga
    14761 ccgcttccaa accttcttcg atgcgcttcg cgatgcgaat ctcgccttcg cgtgtcaaca
    14821 attccactgt gcccatttcg cgcatataca tgcggacagg atcggtggtg cggccgaatt
    14881 cggcgtccag attggcgagc gcagcggtgg cttcttccac cgcctcttcg tcgggggcgg
    14941 cggttgcgtc ggtcagcagc aaagactcgg catccggcgc cttttcgtac accggaatgc
    15001 ccatgtcgtt gatcatgttg acgatgtctt cgatctgctc gggatcgacg atctcgctcg
    15061 gcagatgatc gttgacttca gcgtaggtga ggtagccctg ctccttgccg cgcgcgatca
    15121 acaacttcag ttgcgactga cgctcgtccg cgatttgtgt cgctgtgccc gctggcgctt
    15181 ttttgatcaa ggtggacgcc tcgttggaaa cttagagaat ttaaatgcaa gccgacaagt
    15241 atacgattcc aggcaattcc aagccacaga tgcttgccgc tcaatgggcc aatcgagagc
    15301 ggcccttgaa tttgagaagt tcacttaatt cggccttttc gtcgtaattc aagcccaatt
    15361 cctctgcttt tcgcaataat tcgtccattc ggcggcccgg accgtattcc tcgagcaact
    15421 tctctacggc catttgcagc tccttggcgg ccgcctcggc ggtggccacc aagggctcgg
    15481 ccatggcaag ttccgccaaa cgccgaaatt cgggcagttc ccgaaagcgc tcgagcagca
    15541 gtgccgtggt cggattcgcc gccgccgccg cctgggtcaa gagttcctcg agcacggcca
    15601 cgccagcctt gtcgacgctg cgcagcgctt gcggatcctt aaccgcggcc gccgcgctcg
    15661 gatggtgcag aaccagggta atggcctggg tcagcaaatt accccttccg gcactcattc
    15721 gaccgcgctg tggggcagtc ggttcgcgcc gctgggcggg tttcgcttcg ccgctcgcaa
    15781 acgaccgctt gagcgtcgcc gccggcatgc cgacgcgcgc ggccaggccg tcggccagca
    15841 tttcccggta gataccctcg ggcagccgcg cgaacagggg cgccgccagg gatttgagct
    15901 tggcgcggcc gtcgacatgc tccagatcca cctcggcggc gagctgttgg atcaagtatt
    15961 cggatagggg cagcgcttga ctcagccgcc tctcgaacgc ctccgaccct tccgcgccaa
    16021 ccagcgtatc ggggtcgtga ccgtccggta agaacatgaa cttgagctcg cgtccgtcgc
    16081 gcgcgagcgg cagggaattc tccagcgcgc gccaagcggc ttgacggccg gcacgatcgc
    16141 cgtcaaaaca gaacaccacc tcactggtca gcctgaagat tttgttgagg tgctcctggg
    16201 tcgttgccgt gccgagcgtc gccaccgcat aggtgatacc cgcttgatgc aatcgcacca
    16261 cgtccatata gccttcgacg atcagcaggc gtttcaaatc ggcccgcgct tggcgcgcct
    16321 cgtacagccc gtagagttcg cgccctttgt gaaacaaagg cgtttcgggg gaattcaggt
    16381 acttcggttc gccttgatcg atgatccgac cgccgaagcc gatgactcgc ccgcgcgaat
    16441 cgcgaatcgg aaacatcagc cggtcgcgga atcgatcgta aaacccggca gcgcgttcgc
    16501 cgccacgggt gtcgcgttcg atgatcaagc caagctcgcg aatttggcgc cgttcatctt
    16561 cctgggcgcc aaagcggttg agcaaggcat cccatgaatc cggggcatag cccaagccga
    16621 agcgggcgct ggttttcgcg tcgattcctc ggccggcgac atactgctgg gcgcgcgagt
    16681 tgtccgccag attctgctcg aaaaagcgcg cagcgcgcgc catgacctcg tgcaggtcgg
    16741 cgctgcccgg atcgcgcggc gcctgcgctt cgcgcggcag ttcgagtccc gcgcgctgcg
    16801 cgaggtcggc caccgcatcg aagaattcca ttttctcgta ttgcatgaga aagccgattg
    16861 cggtgccatg cgccccgcat ccgaagcagt ggtagaactg cttgtcggga ctgacccaga
    16921 acgacggtga tttctcgctg tggaaggggc aacaggactt atattcgcgg ccggccttct
    16981 tcagcggcac ccgcgcgttg atgatctcga cgatatcgga gcgcgcgacg acttcgtcga
    17041 taaacggctg tgggatacgg ccggccatgg accgcctgcg atcaaccgcc gagcttggct
    17101 ttgattttcg ccccgacggc ggccatgtcg gcgcggccct gcgccctgcc cttgatgacg
    17161 gccatgacct tgcccatgtc cttgacgctg gcggcagcgg tcgccgcaat ggcctcgccg
    17221 atcagcgcgt cgagctcgga atcactcaat tgggacggca gataaccctg cagcagcgtg
    17281 atttccgctg cctccttctc gaccaggtcc tgacgattgc cggcttggaa ttgcaccagc
    17341 gactctttgc gctgcttgat cattttctcg agcacgctca acacctgggt gtcgtcgagg
    17401 gtgatccgct catccacttc gcgttgcttg atggccgcgg tgatcatgcg aatcaccccc
    17461 agccgctgct tttcgccgga gcgcatcgcc gccttcatgt catccgtgat tcgctcttta
    17521 agagacatcc ttgcgcgggc ggcccctagt aggaaggccg tgccggggct gccggcgggg
    17581 gagtcgacgg cgggccgccc gtgccacgca tgttctcgcg tgacaggcgc ttcaagtgcc
    17641 tttttaccgc agcagccttt tttcgcttac gttcttgggt gggcttctcg tagaattcac
    17701 gtcgccgaag ctcggtgagg acccccgcct tttcgcaggc gcgcttgaag cgacgcaggg
    17761 cggcgtcgaa atactcattc tcgcgaacac gaacgctggg cattcagacc tcaaaatggt
    17821 tggcgcagcc gccggcattc ggcgacaggg gggcgcattc taaggtgcgc ccccataaaa
    17881 ggcaaaggct gccgggcggc ttttagggga ttagggccga ctatgcgcgt tatcgggatt
    17941 gagagctctt gcgatgagac ggcggtcgcg gtctttgacg ccgatgaggg gctgctgtcg
    18001 catcgtctgt acagccagat cgcaatgcac caggcctacg gcggcgtcgt gccggaattg
    18061 gcgtctcgcg atcatgtcag gcggctgctg ccgttggtcc gcgaggcgct cgcggatgca
    18121 cactcggacc ggcaatcgat cgacggggtg gcctataccg cgggtccggg cttgatcgga
    18181 gccttgctgg tgggagcggg ctttgccgcc agcttgggct atgcctgggg caagccggtg
    18241 ctcggtattc accatttgga agggcacttg ctcgcgcccc tcttggagcc caatccgccc
    18301 aggttcccgt tccttgcgct gcttgtttcg ggtggccaca cgcaactcgt cgatgtggcg
    18361 gccctcggtg aatatcgaat cctcggcgaa accttggacg atgcggccgg cgaggcattc
    18421 gataagaccg cgaagctcct cggactgccc tatccgggag gtgcggcgct cgcacagctg
    18481 gccgaatccg ggcgcagtgg cgcttatgtg tttccccggc cgatgctcga tcggcccggc
    18541 ctcgacttca gtttcagtgg cttgaagact gccgccctag tagccttgcg gggccgcgta
    18601 ttggac
//
LOCUS       DASWZT010000420         4698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_51611, whole genome shotgun sequence.
ACCESSION   DASWZT010000420 DASWZT010000000
VERSION     DASWZT010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51611"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VGE92_11935"
     CDS             <1..340
                     /locus_tag="VGE92_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314584.1"
                     /translation="CTRVHGADRGGELRDALALGVARVVERSGRITGYTTQIAFFGHA
                     VCETTDDVQALISAAETFAGPGFLVPSRNGQLMRWCLAKGLRIKQSFTLMTMGLYNEP
                     AGAYLASVLY"
     gene            467..694
                     /locus_tag="VGE92_11940"
     CDS             467..694
                     /locus_tag="VGE92_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314585.1"
                     /translation="MLHKFQAEIKHSPPVTMLHTLTCPVCRSSAEDRRVVRARTVSEA
                     VLDLIATYMSDGVRRSVSVCNCAKGGMPLAD"
     gene            complement(713..2443)
                     /locus_tag="VGE92_11945"
     CDS             complement(713..2443)
                     /locus_tag="VGE92_11945"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEY1314586.1"
                     /translation="MSNHLLLEAAVRSGVMGAIIFAALRLMRIEQVSARRTAWLLALA
                     AALLMPLLVGTQIGPRLLPDVEGFGASRVAVSPEVPGGPASAAAGERTATAAAGDGVG
                     EDSARSASDAGPLLAVGYCIVAAVLSLRLCVGVGWALRLRNQAERIQCAFDPQLDIRL
                     SRRIASPITVASSVLLPANYARWDEPTLHMVLAHERAHVRQGDFYVQLLAGAHCAVFW
                     FNPFSWWLRRQLSDLGEALSDRAAVEHAASRASYAEILLAFASGARWPLAGVAMASAS
                     NLAARIERLLNDNGFERCFDEKRRSAFIAAGVVLMAIAAATSTMQVRAESTGTNTAAP
                     SVVRQQEDPSRDTDAAGDTVSNANGDIDVHDAKVDAKEGVLAIHAGKSRVTINTGNVL
                     PAPAGDYIYFQHDGKPYLIQDPKVISQAQALLAPMDDLERQQKELGKRQALLGAQQRQ
                     LGAQQRAIKVESPDFKREIAEIENVIKQMNLAQASAQIDQNALAHLQAHLGEIQSRVA
                     ELQSDLGRQEGSFGGKQGELGEQQAELGEQQALLAEQSRKIVELMRRQMRPILEQAIR
                     DGKGKALVNW"
     gene            complement(2440..2850)
                     /locus_tag="VGE92_11950"
     CDS             complement(2440..2850)
                     /locus_tag="VGE92_11950"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEY1314587.1"
                     /translation="MTSNTTQGHDPAELGELERAVLELIWEHGVQSADQVREHLARHG
                     RPLKESTIRTVLRRLEEKGYLAHSVDSRTFLYRPAASRQAVAGRAVKRIVDWFCEGSV
                     ETLLAGMVDSAVLDRKELQRLAERIAAAKEGKKS"
     gene            2931..3476
                     /locus_tag="VGE92_11955"
     CDS             2931..3476
                     /locus_tag="VGE92_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314588.1"
                     /translation="MKKIAPRDFRNAFVEVMKSEHDSFRTAVGFEKKSYNYFIRSTIY
                     PRIARQLGLLAWNKEYLTLDGMFYEERGVDDTGKYAAYANWISVAVEHENEAMKADET
                     MNKLQLFNAPLKVLITYAADGSGTESLLRKYENIIRASDVFNDVATLRQQLVILGTPK
                     TVGEWRFYAYENDGFVLMLPN"
     gene            complement(3523..>4698)
                     /locus_tag="VGE92_11960"
     CDS             complement(3523..>4698)
                     /locus_tag="VGE92_11960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY1314589.1"
                     /translation="AAAAAGAREFPEVHLEGAAAAQRQSLNSPLGKIASAFPGYERDQ
                     TLRDIDAGASWEIDLFGGLRRGVQAAAAEAQAAAALQDGVRISVAAEAADAYFQLRGF
                     QARIALTEAQIDIDQRLLGLVHQRQASGVSADLESAQAQALLAHARGALPPLKSGLAV
                     QLNRLDVLMGAAPGTYSAELRASSADYTVPAIDTGDGPAQLLRQRPDVIAAERHLAAS
                     SARIGVAVAEYYPKVSLTALLGFESLNGAALVQSASFQPAAAAGLRWRLFDFGRIDAQ
                     VAQAKGANAEALAEYRQSMLRATEDVENAIVGVTQLEAQYDELREEVAANDRARAAAQ
                     RQYEAGAVSLLEVLDSDRQLLISRDALAQVHAEDARAAVETFRALGGGWTAARRESR"
BASE COUNT          904 a         1496 c         1401 g          897 t
ORIGIN      
        1 gtgtactaga gtccatggtg cagaccgcgg cggcgagctg cgcgatgcgc tcgcccttgg
       61 cgttgcccgc gtcgtcgagc gcagcggacg catcacgggc tataccacgc aaatcgcatt
      121 tttcggtcat gccgtgtgcg aaacgaccga tgatgtgcag gcactgatca gcgcggcgga
      181 aacgtttgcg ggtcccggct tccttgtgcc gtcgcgcaat ggccagctga tgcgctggtg
      241 tctggccaag ggattgcgca tcaagcaatc tttcactctg atgacaatgg gcctctataa
      301 cgagcccgcc ggggcgtatt tggcgtccgt tttgtactga aatccctgcc agacggtgct
      361 gatcgagcga tttccgccgc gagtgtcagg tcccggcgac accttttaac ataagcagaa
      421 accaagtagc ttctcaacct cgctaaacca acgggggatc agtcacgtgc tgcataaatt
      481 tcaagctgag attaagcatt ctccgccggt gaccatgttg cacaccctca cctgtccggt
      541 gtgccgcagc agcgccgagg accgccgggt ggttcgcgca cggaccgtct ccgaagcggt
      601 gttggatctg atagcgacct acatgagcga cggggtccgg cgcagcgttt cggtatgtaa
      661 ctgcgccaag ggcggaatgc cgctggctga ttgaagcaga cgcgcgagtc gtctaccaat
      721 taaccagtgc cttgcctttg ccatcgcgga tcgcctgttc gagaatcggc cgcatctgcc
      781 gtcgcatcag ttcgacgatc tttcgactct gttcggcgag cagtgcctgc tgctcgccca
      841 actcggcttg ttgttcgccg agttcaccct gcttcccgcc aaagctcccc tcttggcgcc
      901 ccaaatccga ctgtagttcc gccactcgag attgaatctc accgagatgc gcctgcaagt
      961 gcgcgagggc gttctgatca atctgtgccg aggcttgcgc gagattcatc tgtttaatca
     1021 cattctcgat ttcagcaatt tcgcgtttga agtcgggaga ttccaccttg atcgcccgct
     1081 gctgggctcc tagctgccgt tgctgcgcgc cgagcaatgc ctgccgtttg ccaagctcct
     1141 tttgctgccg ttccaagtcg tccatcggcg cgagcagcgc ttgagcttgc gaaatgacct
     1201 tcgggtcctg gatgagatat ggcttgccat cgtgctgaaa ataaatatag tcgcccgccg
     1261 gcgccggaag cacgttgcct gtattgatcg tgacccggga tttaccggcg tggatggcga
     1321 gaactccttc tttggcgtct accttggcgt cgtgtacgtc gatgtcgcca ttggcgttgc
     1381 tcaccgtgtc gccggcggca tcggtatcgc gcgatggatc ttcttgctgg cgaacgacgc
     1441 ttggcgctgc ggtatttgtc ccggtcgact cggcgcgcac ctgcatcgtc gacgttgcgg
     1501 ccgctattgc cattagcacc acgcccgcgg cgataaacgc tgagcgccgc ttttcatcga
     1561 agcaacgctc gaagccgttg tcattcagca gccgctcgat gcgcgcagca agattgctgg
     1621 cgctcgccat cgcaacgccg gcgagaggcc agcgggcgcc gctcgcaaag gcaagcaaga
     1681 tttcggcgta cgatgcgcgg ctcgcggcat gctcgaccgc ggcacgatcg ctgagcgctt
     1741 cacccagatc ggatagttgg cggcgcagcc accaactgaa aggattgaac cagaaaaccg
     1801 cgcagtgcgc tcccgcgagc agttgcacgt aaaagtctcc ctgacgaaca tgcgcgcgtt
     1861 catgtgccag caccatgtgc aaggtcggtt catcccaccg tgcatagttg gcgggcagca
     1921 gcacactcga cgccacggtg atgggactcg caatccgccg gctgaggcgt atatccaact
     1981 gcggatcgaa cgcgcattgg atgcgctcgg cttggttgcg caggcgcagc gcccagccca
     2041 ctccgacgca cagacgcagc gacaagaccg ccgcgacgat gcagtagccg accgcaagca
     2101 gggggccggc atcgctcgcg gatcgggcgc tgtcctcgcc cacgccatcg ccggccgctg
     2161 cagtcgccgt gcgttcgccc gctgccgccg acgcggggcc gcctggcacc tcgggcgaga
     2221 cggcgacacg tgatgcacca aacccttcaa catcaggcaa cagccgcggc ccgatttggg
     2281 tgccgaccag cagcggcatc agcaacgcgg cggcaagcgc caacagccag gcggtgcgcc
     2341 gcgcgctcac ttgctcgatg cgcatcaggc gcagcgcagc gaaaatgatc gcgcccatca
     2401 caccggagcg cacagcggct tcaagcaaca aatgattgct cacgattttt tcccctcttt
     2461 cgcagcagcg atacgttcgg caaggcgctg aagttccttg cggtcgagga ccgccgaatc
     2521 aaccatgccg gcaagaagcg tttcgaccga gccttcgcaa aaccaatcga cgatgcgttt
     2581 caccgccctc cccgccaccg cttggcgcga cgccgccggc ctgtacagaa aggtgcggct
     2641 gtcgacggaa tgagcgaggt agcccttctc ctcgagccgg cgcagtacgg tccggatggt
     2701 ggattccttg agcggacgcc cgtgccgcgc cagatgttct cggacttgat cggcgctctg
     2761 cacaccgtgc tcccaaatca gttcgagaac ggcgcgctcc agctcgccca attccgcggg
     2821 atcgtgacct tgcgtagtgt tactcgtcat agtgttactg actgtagcat atagaattaa
     2881 tttggcaagt accgtcctag cctcatgaaa tgcaatctaa tgaacccacc atgaagaaaa
     2941 ttgcgcccag agactttcga aatgctttcg tcgaggtcat gaagtcggaa cacgacagct
     3001 tccggacggc ggtcggcttc gagaagaaat cctataacta cttcatccga tccacgattt
     3061 atcccagaat tgccaggcag ctcggcttgc tcgcctggaa caaggagtac ttaacgctcg
     3121 acgggatgtt ctacgaggag cgcggcgttg acgacacggg caaatacgcc gcgtatgcaa
     3181 attggatctc ggtcgccgtg gagcacgaaa acgaggccat gaaagccgac gagaccatga
     3241 acaagctgca gttattcaac gccccgctga aggtgttgat cacttatgcc gcggacggca
     3301 gcggcaccga gtcgctattg cgaaagtatg agaacatcat cagagcatcc gatgtgttca
     3361 atgacgtcgc tactttgcgg cagcaattgg tgatactcgg aacgccgaag acggttggag
     3421 aatggcgctt ttatgcctac gaaaacgacg gcttcgtgct gatgttgccg aactgaacgg
     3481 gaaattcgca ttcaatcctt cgggctgcaa accccaacca tactagcggg actcacgccg
     3541 cgccgcggtc cagccacctc caagggcccg aaaggtctcg acagcggcgc gcgcatcttc
     3601 ggcatgaacc tgcgccagcg catcgcgcga aatcagcaac tgccggtcgg aatcgaggac
     3661 ctccaagaga ctgacggcgc cggcctcgta ttgccgctgc gcggcggcgc gcgcgcggtc
     3721 gttggcggcg acttcctcac gcagttcgtc gtactgcgcc tccaactggg tcacgccaac
     3781 gatcgcgttc tcgacatcct cggtggcgcg caacatcgac tgtcggtatt cggccagcgc
     3841 ttcggcattg gcgcccttcg cctgcgcgac ttgcgcgtcg attcgcccaa aatcgaatag
     3901 gcgccagcgc agccccgcgg cggccgccgg ctggaacgag gcggactgaa ccaaggctgc
     3961 cccgttcaga ctctcgaatc ccagcagcgc cgtgagcgac acctttggat agtactccgc
     4021 gacggcaacg ccgatgcggg cgctggaggc ggctaggtgc cgttccgcgg cgattacgtc
     4081 cggacgttgg cgcaaaagct gcgcgggtcc atcgccggta tcgatcgccg gaacggtata
     4141 gtctgccgat gatgcgcgca gttcagcgct atacgtaccg ggcgcagcgc ccatcagcac
     4201 atccagccgg tttaattgca cggcaagtcc gctcttcaac ggcggcagag cgccgcgcgc
     4261 atgcgcgaga agcgcctgcg cttgcgcgga ttcgagatct gcagacaccc cactggcctg
     4321 cctctggtgg acaaggccca gcagccgttg atcgatgtcg atctgcgctt cagtcagcgc
     4381 aatccgagcc tggaatcccc ggagctgaaa atacgcatcg gccgcttcag ccgccacaga
     4441 aatgcgcacc ccgtcttgca aggctgcggc ggcctgggct tcggctgcgg cagcctgcac
     4501 accgcggcgc aacccgccga acaggtcaat ttcccagctg gctccggcgt cgatatcgcg
     4561 caacgtctgg tcgcgctcgt agccgggaaa ggcgctcgcg atcttgccga gcgggctgtt
     4621 gagcgattgg cgctgcgcgg cggcggcgcc ctcgaggtga acctccggaa attcccgggc
     4681 acccgccgcc gcagccgc
//
LOCUS       DASWZT010000421        10916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_80741, whole genome shotgun sequence.
ACCESSION   DASWZT010000421 DASWZT010000000
VERSION     DASWZT010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10916
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80741"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /gene="wrbA"
                     /locus_tag="VGE92_11965"
     CDS             <1..553
                     /gene="wrbA"
                     /locus_tag="VGE92_11965"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF002999.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEY1314590.1"
                     /translation="ADGAARVSGTAVSVKRVPELMPVEVATAAGCKLDQPAPIADPLE
                     LADYDAVLFGTPTRFGNMASQMRNFLDQTGPLWARNALVGKIGSVFCSTASQHGGQET
                     TLTSFHTTLLHHGMVVVGLPYTFKDLMTLREVTGGTPYGASCVTGAGGELRMPSALEL
                     EMCRFQGEHVARITARMAASASV"
     gene            550..1584
                     /locus_tag="VGE92_11970"
     CDS             550..1584
                     /locus_tag="VGE92_11970"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEY1314591.1"
                     /translation="MTLLPTTIVGSYPQPEWLIDRSKLAGRFPPRVRARELWRISPEY
                     LEEAQDDATILAIKAQEEAGIDIVTDGEMRRESYSNRFATALAGVDIDNPGSALDRSG
                     HPNPVPRVVGPIRRRHAVEVEDVNFLRRHTTRKIKISVPGPFTMAQQAQIDYYDGSRE
                     LAAMDYAAAVNAEIRDLFRAGADVVQIDEPYMQARPEEARAWGQKALNRALEGVAGVT
                     AVHICFGYAAIIHTRPSGYSFLPELQNCLCNQISIETAQSNLDCSVLREFRGKKIMLG
                     VIDLSDMQVESAAMVAARIRRALPFIDARDVIASTDCGMKYLPREVAAGKMRALVEGA
                     RIVRQELERG"
     gene            1589..2230
                     /locus_tag="VGE92_11975"
     CDS             1589..2230
                     /locus_tag="VGE92_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314592.1"
                     /translation="MRIRSVMGWLVISFCPLAQSGSRDAAPVSVADVSALLAGRYDNA
                     AQLELNKPAAGDPPLQHVTVTIELTQRAEWELWRIHMDVDPAVANSAGSETSLNALWA
                     MNILRRKAGQPLELIPYTLKPSVSEASVNAAAFDQAQWLSLEACALGGNFTKSRAVVQ
                     APADEMCVAETMGLGGKRAFLPSWVERDGDRLGVELIYFGKPWRVDARRVQAQ"
     gene            2241..4448
                     /locus_tag="VGE92_11980"
     CDS             2241..4448
                     /locus_tag="VGE92_11980"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEY1314593.1"
                     /translation="MAAPAAAIVVRFVSLTLLAGIFTAPSHAAAPCTGQLSSADSIIA
                     VEAGDAAPRLSRLTLRGGASWENRTDEPLPGRVEVNGVSQSMTWKLDRASCRFDPSRI
                     DLVYISASPRLELTWEWRRRAQRGPLEHRIVIRNASGESIWLPWQPSFKFAWAVDIAQ
                     PLERFWVEKGADAPSSAGTHRDALREGDHWFGVSSTYARPLAHQPREMIPYLLVDDPA
                     GSGAGWYLGIEFSGRTRITLERAGDHLSGTAGLNPDPGPYRTRLPPAGVFETPTIFLG
                     AFAGGADDAGNILRRWIRAVLNNPRTVRDPTYPTLVNNSWGSGMAVDEALAKRMIRES
                     RRLGLEMYHLDAGWFRGVGDWHADSAKFPSGIASLADFAHAQGLKFGLWVDFTQAGVS
                     SRPGARNVNDPETRDWLIGDPPDNWQHREQFKGITMDLGVPAAQAWALAEVSRLVTEN
                     HLDMLEHDGYLVAQGSSRSDHPAAAPAPGTLREYEDSGYLWVDGSNSTDVSDHATRAY
                     YAIYERLRLEHPRLLLELCNDGGRMVDFGSAAHGDYFSITDVYDPLSNRRAFFDASFV
                     LPPAMLESYVEKWPTPTLDNFRYMLRSGMMGWFSLMQDSAEWSAQQHAAAAAEISLYK
                     SALRPLIRTADLYHVAERPDGVHWDGIEYFSSSAARGVLFAFRGSTTETAHRYLLAGL
                     DAERSYRITFHDRGAAATAILSGATLMRDGVEVSLATALSSELVFIDGGRTAR"
     gene            complement(6959..8803)
                     /locus_tag="VGE92_11985"
                     /pseudo
     CDS             complement(6959..8803)
                     /locus_tag="VGE92_11985"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8802..10601
                     /locus_tag="VGE92_11990"
     CDS             8802..10601
                     /locus_tag="VGE92_11990"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY1314594.1"
                     /translation="MPFITASAAENREFDVIVVGSGAAGGQTAYTLALEGVQVLMLEA
                     GRRYSPDSETPMFHTPFMAPLRGESTPDKPFGFYDATIDGGWQVPGEPYSSASDDPLR
                     QFWWWRPRMLGGRTNHWGRIALRNGPYDFKPYSRDGLGRDWPVSYEQIAPYYDRVERL
                     IGVYGSNEGLENTPNSSPGVLLPPPKPRVGELLAQKAARRLGVPFIPMHRAVMTKRLD
                     DRRLPALLHPGNLTAQRILAADMRRRAACLYATPCGIGCSVRANYQSTTVHLPPALAS
                     GNLHIVCDAMVREVVVGKSGRATGVRFIDKTTGQDRRARSRAVVLAAGACESVRILLN
                     SARDTGGLANSSGLVGKYIMDTVGSNPGGQIPRLEALPPHNEEGADGEHLYVPWWLYG
                     KDNIRKLGFARGYHIEYGCGRRMPGLGTGQRLEDFTGGSYGASFKADVRRYYGSFVGF
                     AGRGEMIPNQDCYCEIDPNSKDRYGIPVLRFHWLWSEHETRQAAHMQQTFAAIIDAMG
                     GHVRGAVEHDGRKAIEPGGKIIHEVGGTIMGDRPQDSVTNQWGETWDVPNLVVTDGGT
                     FCSNADKNPTLTIMALAWRCADHMLERLKRREI"
     gene            10603..>10916
                     /locus_tag="VGE92_11995"
     CDS             10603..>10916
                     /locus_tag="VGE92_11995"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEY1314595.1"
                     /translation="MNQPPRIDRRTTLKWVLGAAASLPLLIKVRAASHGAGYGTDPNL
                     MPLYRPGELWPLTLNIGERRSAAALSDIIIPADDNSPSASAVGVVDFIDEWISAPYPD
                     QR"
BASE COUNT         1980 a         3500 c         3468 g         1968 t
ORIGIN      
        1 ggctgacggc gccgcacgcg tctcgggcac cgcagtatcc gtcaagcgcg ttcccgaact
       61 tatgccggtc gaggtggcca ccgccgcggg ttgcaaattg gaccagcccg cgcccattgc
      121 agatccactc gagctcgcgg actatgacgc cgtcctgttc ggaacgccca ctcgcttcgg
      181 caacatggct tcgcaaatga ggaatttcct cgatcaaact ggaccgctgt gggcgcgcaa
      241 tgcgctcgtt ggtaaaatcg gcagtgtgtt ctgcagcacc gcgagccaac atggcggaca
      301 ggaaacgacg ctgacctcct ttcacaccac tcttctgcat cacggcatgg tggtggtggg
      361 gctgccatat accttcaagg acctgatgac tctgcgtgag gtcaccggcg gcacgccgta
      421 cggtgccagt tgtgtgaccg gagccggcgg cgaacttcgc atgccctcgg cgttggaact
      481 cgagatgtgc cgctttcagg gagagcacgt ggctcgcatc accgcccgga tggccgcgag
      541 cgcgagcgta tgacgctcct tcccaccacg attgtcggca gctaccccca gcccgaatgg
      601 ctcatcgatc gctcgaaact cgccggccgc tttccgccgc gggtacgcgc tcgcgagttg
      661 tggcgcatct ctcccgagta tctcgaggag gcgcaggacg atgcgaccat cttggcgatc
      721 aaggcgcaag aagaagcggg aatcgacatc gtcaccgacg gcgagatgcg gcgcgagagc
      781 tactcgaatc gatttgccac ggcgttggct ggcgtggaca tcgacaatcc cggatcggcg
      841 cttgaccgca gcggtcatcc gaaccccgtg ccccgggtcg tcgggccgat tcgccgccgg
      901 catgccgtcg aggtcgaaga cgtcaatttt ctgcggcggc acacgacccg aaaaatcaag
      961 atctcggtac cggggccttt taccatggcc caacaggcgc agatcgacta ctacgacggc
     1021 agccgtgaac tcgccgccat ggattatgcg gcggcggtca acgcggagat tcgcgatttg
     1081 ttccgcgccg gtgcggacgt cgtgcaaatc gatgaaccct acatgcaagc gcgcccggag
     1141 gaggcgcgcg cctggggtca gaaggcgttg aaccgtgcgc tcgagggcgt cgccggcgtg
     1201 acagccgtgc acatttgctt cggctacgcg gccatcattc atacgcgtcc cagcggttat
     1261 tcgttcctgc ccgaactgca gaattgcctg tgcaatcaga tttcgatcga gaccgcccaa
     1321 tccaacctcg attgctccgt acttcgcgaa tttcgcggca agaaaatcat gctcggcgtc
     1381 atcgacctgt ccgatatgca ggtggaatcg gccgccatgg tcgcggcgcg cattcgccgc
     1441 gcgctgccgt tcatcgacgc ccgggatgtc attgccagta ccgactgcgg catgaaatat
     1501 ctgccgcgcg aagtcgccgc cggtaagatg cgggcgctgg tcgagggggc gcgaatcgtt
     1561 cgtcaagagc tcgagagagg gtagatccgt gcgcatacgt tcagtgatgg gctggctggt
     1621 catttcattc tgtccgcttg ctcaatccgg ctcgcgcgat gccgcgcccg tctcggttgc
     1681 ggatgtcagc gcgctgctgg cgggacgcta cgacaacgcc gcgcaactcg agctgaacaa
     1741 gccggcggcg ggcgatcctc cgttgcagca tgtcaccgtc acgatcgagc tcacgcaacg
     1801 ggccgagtgg gaactttggc gcattcacat ggatgtcgat cctgccgtgg caaattccgc
     1861 cggatccgag acctcgctca acgcgctctg ggccatgaat atcttgcgcc gcaaagccgg
     1921 ccagcctctc gaactcattc cgtacacgtt gaaaccctcc gtcagcgaag cgtcggtgaa
     1981 tgccgccgca ttcgatcagg cgcagtggct gtcgctcgag gcgtgcgcct tgggcggcaa
     2041 tttcaccaaa tcgcgggctg tcgtgcaggc gcccgccgat gagatgtgtg tagccgagac
     2101 catggggctg ggaggcaagc gcgcctttct gccgagctgg gtcgaacgtg atggcgatcg
     2161 gctgggcgtc gagctcattt atttcggcaa gccatggcgc gtcgatgcaa gacgtgtgca
     2221 ggcgcagtga gagcccctgc gtggcggctc ctgcagctgc catcgtcgtt cgctttgtat
     2281 ctttaacatt actcgccggc attttcaccg cgccgagcca tgcggcggcg ccgtgtaccg
     2341 gacaactgtc cagcgccgac tcgataatcg ccgttgaggc gggcgatgcg gctccgcgct
     2401 tgtcccgact caccttgcgc ggcggcgcat cctgggagaa tcggaccgat gagccgctac
     2461 ccggccgggt cgaagtgaac ggcgtctcgc agtccatgac ctggaaactc gaccgcgcct
     2521 cgtgccgctt cgatccctcg cggatagacc ttgtctacat ctccgcctcg ccacgcctcg
     2581 agctcacgtg ggaatggcgc aggcgcgctc aacgcgggcc gctcgaacac cgcatcgtga
     2641 ttcgaaacgc gagcggcgaa tcgatctggc tcccgtggca accgagtttc aaattcgcct
     2701 gggctgtcga catcgcgcag ccgctcgagc gtttctgggt ggagaaaggc gcggacgcgc
     2761 cctcctccgc gggcacccat cgggatgcgc tgcgcgaggg cgatcattgg ttcggtgtat
     2821 cgagcactta cgcccgcccg ctcgcccatc aaccgcgcga aatgattccc tacctcttgg
     2881 tcgacgaccc cgcgggcagc ggcgcgggct ggtatctggg gatcgagttc agcgggcgta
     2941 cgcgaatcac gctcgagcgc gcaggcgacc acctgagcgg gacagcgggc ttgaatcctg
     3001 atccgggacc gtaccgcacc cgcctgccgc cggccggtgt tttcgagacg ccgacgattt
     3061 ttctgggggc atttgccggc ggtgccgacg atgcgggaaa tatcttgcgc cgctggatcc
     3121 gcgcggtcct caacaatccg cgcaccgtgc gcgatccgac ctatcccaca ctggtgaaca
     3181 acagctgggg cagcggcatg gccgtcgatg aggcgctcgc caagcgcatg attcgcgagt
     3241 ctcgccgctt agggctggag atgtatcact tggatgcggg ctggtttcga ggcgtgggcg
     3301 actggcacgc ggactccgca aagtttccaa gcggcatcgc ctcgctcgcc gatttcgcgc
     3361 acgcccaagg gctcaagttc ggcctgtggg tggacttcac tcaagcgggc gtgtcgagcc
     3421 ggccgggcgc gcgcaacgtg aacgatccag agacgcgtga ctggttgatc ggcgacccgc
     3481 ccgacaattg gcagcatcgc gaacagttca aaggtatcac gatggacttg ggcgtccccg
     3541 cggcgcaagc gtgggcgctc gcggaagtct cgcgtctggt gacagagaat cacctcgaca
     3601 tgctcgagca tgacggctac ctcgtggcgc agggcagctc gcgcagcgat catccggcgg
     3661 cggcgcctgc gccgggcacc ttgagggaat atgaggattc cggatacttg tgggtcgacg
     3721 gctccaactc gaccgatgta agcgatcacg ccacgcgggc gtattacgcc atttacgaac
     3781 gcctgcgact cgagcaccca cgtctattgc tcgagttgtg caatgacggg ggccggatgg
     3841 tggactttgg cagtgccgcg cacggagatt atttctccat caccgatgtc tatgatccgc
     3901 tgtcgaatcg ccgcgctttt ttcgatgcca gttttgtact gccgcccgcc atgttggaaa
     3961 gctatgtcga gaagtggccg acgccaacgc tcgacaactt ccgctacatg ctgcgcagcg
     4021 gaatgatggg ctggttttcg ctgatgcagg attcggcgga gtggtccgcg cagcaacacg
     4081 cggcagccgc ggccgaaatc tcgctctaca aatccgcctt acggccgctg attaggacgg
     4141 ccgatttgta tcacgtggcg gagcgcccgg atggcgtgca ttgggatggt atcgagtatt
     4201 tctcgagcag cgccgcgcgc ggcgttctgt tcgcgttccg cggcagcacg accgaaaccg
     4261 cgcaccgtta cctgctcgcg ggcctcgatg cagaacgctc ttaccgcatc acattccacg
     4321 accgaggcgc ggcggcaaca gccatcctgt cgggcgccac gctgatgcgc gatggggtcg
     4381 aggtcagctt ggcgactgcg ttatcctcgg aattggtctt catcgacggg gggcgcaccg
     4441 cgagatagac ggtgctccag agctgtgccg cgttcgactc atcggcatcg gccccggcgg
     4501 caccgaacgc ggcgatccga taatggccgt cgccaaatga ccacgaccaa agctccgagt
     4561 tgttcatgga gcgcgaagca tcaatggcct gccacagcag ttgctgcgcc ttgagcaaat
     4621 ttgcccgcac tgccggggcc agatcactgc ggctgagttg tcgctgcaag cccgccgcga
     4681 acagcgcctg ttgccaagac cacaccacga cgccgtgata ggcattgcgg ctgaatctct
     4741 cttgaacgtc ggtcgccgcg tacaccgggt ccgcgaccaa catgccgacc tcggtcatca
     4801 ggccggcggg aaacggcttc aacagcgacg ctgcgtcgcg tctcaagtgc tcggcgtccg
     4861 gctcgccgaa caacagggcg aacccctcat cggaattcat gatcggcaca ggtgcgccat
     4921 tgacatcgag cgacagtgcg tggaagcgca cagcgccggc ctcgagcgat gccagggcgg
     4981 gttgcgccgg aacgccgagg gaggccgcat accgcgtgat cgcggcagtg gcggtggcgt
     5041 gcgccacggc gacgtcgaac agcggcgggg cttgtgtgtt ccagatcttg gccatcgcag
     5101 cggcgcgcgc aaagagcgtc cggtcggcgg cgctcaaata tggatcgagc agcccgctct
     5161 cgagaaaacg cgccgccgcg gccaaggcgg ccggcaccag gaccgcgttg acgtcatagg
     5221 gaaaacggcc gcgccccagg ccctcgttgc tgtcgcgcca gttaccgacc atgaggccgg
     5281 gcttgatgcc gatcagccgt gaccatcgag gatcatttgc gaatggcatc gcactcgtca
     5341 ataccagacg tagattcgca accagtgcat cgccacggcg tgccgctgcg tggctgtctg
     5401 cggccaaaaa atcctcggcc cgctgccggc cgcgcgcgtc gtccacgagc catgcgcgcg
     5461 ccaccggcgc gagcatgtag ctgccatcga tcatcttgta gtcgagcagc ggcgcatccg
     5521 acttggagcc gtcggtgcgc aggtgatcga gcacggcgaa ttcgccgata ctctcctcgt
     5581 gcgccacttc gcctcgactc gacaggcgct cgagaaccga ccccaatccc gcttccaccg
     5641 cctgcggatt caaaaccggc atcagcagac ggaccgacat caatgtgtcg cggccaaaat
     5701 aggtgtcgaa tctccacgac ccggcaagaa acttttcctg gtagctgaga taggtcagtg
     5761 cgttgcgcgc cgcgtcgtcg gtcgcggcat gctcattcaa caggctctcg ccgtatagcg
     5821 gcgtcaaggg cacctcgccg ctggcgccga tgaccttgag ccgaatcttg ccatcctcgc
     5881 ccgcgcgaat ccgcggattg ggttggttgt cctccaccga cccatcgatg acttccacgc
     5941 tcaagcggta gccggccgcg ccatcgaggc ggtcgcgggc ccacgtgagg gtgtgtcctt
     6001 gcacgaccgg ccggctatcg acgaggctgg gcacggtgcc gagcccttgg taatcgcgca
     6061 gcacccgcac actcgagagc actgcctttt gaacgccaag agccggcgcg gcaatctgcg
     6121 cctcggtgct gatcccgtac agcgggcggc cttgcgcgtc cgctaggacg agcgcttgcg
     6181 gccgctcagt gatttgccac tcggcgcttt tctgcagcgg cgaaaaccac agcccgacgc
     6241 cgctgttgcc ggccggaaag gcgacgatga tgcgcggatt tgcgccggag cggagcaata
     6301 ggtgcgccgc caccttgccg tcgcggacaa aatcgttgat gttgagaccc tcgcgaacct
     6361 cgtagccgaa cggacgagcg gcctgcgacg cggcgctcgc gcccacggcg cgggccaact
     6421 gcaccttgcc gccggcatcg cgtgcgatgc gaatcgtcag ccgctcaccg cggaagctca
     6481 agttctgcag cgtcaacgat ttccatgcgc tcggcaagat cggcggatag gcctgcacca
     6541 gcccctgctc ctggatgcgc agtccactga agccgaacac gaggttctgc acgtatccgc
     6601 ccgatgcggt gagaaagtag ctcgtattgt tggcggccgt ttcggtgcgg acattgaacg
     6661 gcggcttgag cgtgccggtg gtgaagttgg cctggaacca ggccgtggca gcgtcgaggt
     6721 tgctggtcgc ggccgctgcg atcgaggtgg gcgcgaaccc catgctgttt ccgacgtgcg
     6781 gagtcggcga cggcttcacg gcgtaggcat agtcgttgcg ccgcagttcc tcgctcatcg
     6841 gcgcatcgag cgaaggatac agcagcaacg gcagagcgct gcttccggcc cattccttgt
     6901 cggcgacgac cacgctcgga tcgaaatcga tgtggtgctt gccgtccgcg gacatcggta
     6961 cgtacagctc gcgagacagc gtgctccagc gcggatccgc ttgctcgccg accagcgctg
     7021 cggccagcgt cgcgatcttg agcgccttgg cggccgagac attggtgaac gtatcgttgg
     7081 gcacgtcgtt gtaaggttcg ttgaccgagg tgacatgctg gatttcatat cgatggcggt
     7141 ggggaacgta ggtggcacgg ctcacccaga agcgggcgac atcgcgaatc accggccagc
     7201 cggagcggcg cagccagtcc cgatcttcag tggcgaggta atactgccac tgcgcgattg
     7261 cgacgtcggc ggtcacgtgt atctcgcgat cgcccaaaac ggcggcgaaa tgcggtgtct
     7321 gctcggtgcc gttctcggga tcggactccc atgggaacat cgctccggca tagccgtgcg
     7381 cctcggcccg ccggcgcgca ggttccaaag tacggtcgcg aaacatgacc agcgatttcg
     7441 cgcgctccgg atgcaacagc agcaatgccg gaaaaatcca ggtgtcgcta tcccaaaaaa
     7501 tatgctcgac gtatcccgtc gacaggccgc aggcgcccaa cgcccacccg gtatcggcgg
     7561 tcgagctcgc gagcaagtag tagagctcgg agtgcagcac ctgctgtgcc ttggcatcgc
     7621 cgtcgatgag tatatcggtg tgccacagtc gctcccaggc gaggcggtgt tcctcgagca
     7681 agcgctcgaa tccattcacc ctcgcggcgc gcgccagacg caaatcctcg gcggcatcgc
     7741 cgccccaacc ctggcgcgag aaggcgacat acttggtgaa ggcgtaggtg tggcccttct
     7801 cgaccttcac atcgaaattc aaggcgaggc gataggcgct cttatagaca cgcacgttct
     7861 ccgccgcgac acctgcgggc agtgcaaccg ccgcggcctg cgccatggtc aagccttgct
     7921 ccgcttggcc atcgagccaa aggctcaaac tcgccgcatc accttcgctg tgcactacat
     7981 acgtatcgcc cgggtaccac acggcggcac gatccgcggt cgccggggga acgggcttga
     8041 gcgcgagccc gtgtgcggcg acggcctcct ccatctgcgc accgctcagc ttgccgagcg
     8101 gcaatcgcgg cgcgtgcggc gcccacaggt tgaaggcaaa ggacaactgc accgttccat
     8161 cgaagtccgg ggtcatcacc aaacggctgg cggccaagtg cggcgcggcc tgactcacca
     8221 aggtcgtcac ctcgatcgcg gtcgccttgg cgccatccac gtagcgatag cgtgttgtca
     8281 gcgtggcctc atgcagatcg agaacctggc tgtagtcctt gaaacgtgcg tcggagagcg
     8341 acactcgatt gagccattgc tgacccgtta cggtcgcgcc cgtgctgtag tcgatctcgg
     8401 tccaaccggg aacggccgcg ggacgcgaga agtcgtcggc cgtatagtcc atgaatgcca
     8461 ccagatggct catcgcactc tcggtgccgc gcggcgaact gaatgtcgac acatagccgt
     8521 tggcaagata cgtcggaaaa taatcggcga gatccgcctc ggtcgcggac aacaggaaac
     8581 tcggatcggt ggcgcacaac gccggcgtta tccacgcgcc cagcagcagc gcgcaaagcg
     8641 ccgcgaaacc gcaaggccgc gcattactct ttcgatccat ttcttgtgcc tccccgaaag
     8701 cgcttttcaa cagggcgccg atcctagcac tgtggatcga cgttggctta accgcgccgc
     8761 tgcgattaaa ctgagttcgc agcgacttcg ctgaaactac catgccgttt atcactgcca
     8821 gcgcagccga gaatcgagaa ttcgacgtga ttgtcgtcgg ctccggcgcc gcgggcgggc
     8881 aaacggccta cacgctggcg ctcgaaggcg tgcaggtgtt gatgctcgaa gccggccgcc
     8941 gctattcccc ggatagcgaa acaccgatgt ttcacacgcc cttcatggcg ccgttgcgcg
     9001 gcgaaagcac gcccgacaaa cccttcggct tctacgatgc cacgatcgat ggcggctggc
     9061 aggttccggg cgagccttac tcgagcgcca gcgatgatcc gctgcgtcaa ttctggtggt
     9121 ggcgaccgcg catgctcggc ggacgcacca atcactgggg ccgcatcgcg cttcgcaatg
     9181 gtccctacga tttcaaaccg tactctcgcg atggcctggg ccgcgactgg ccggtctcgt
     9241 acgaacaaat cgcgccgtac tacgaccgcg tcgaacgcct gatcggtgtg tacggttcga
     9301 acgagggact ggagaacacg ccgaattcct ctcccggcgt gctgctgccg ccgccgaaac
     9361 cgcgagtcgg cgaattgctg gcgcagaagg cagcacgccg gctcggcgtg ccgttcattc
     9421 cgatgcatcg ggcggtcatg accaagcgtt tggatgatcg gcggcttccg gcgcttttgc
     9481 accccggcaa tttgacggca cagcgcatcc tcgccgcgga catgcgccgg cgcgccgcat
     9541 gtctgtatgc cacgccgtgc gggattggtt gctcggttcg cgccaactat caatcgacga
     9601 ccgtgcatct gccgcccgcg ttggcgagcg ggaatctgca tattgtttgc gatgccatgg
     9661 tgcgtgaggt ggtcgtgggc aagagtggcc gagccaccgg tgtgcgcttc atcgacaaga
     9721 ccacgggcca ggatcgccgc gcccgctccc gggccgtggt actcgcggcc ggcgcctgcg
     9781 aatccgtgcg cattctgctc aattccgcgc gtgacacggg cggcctggcg aactcgagcg
     9841 gcttggtcgg caaatacatc atggacacgg tgggctcgaa ccccggcggc cagataccga
     9901 ggctcgaggc actgccgccg cataatgagg aaggcgccga cggcgagcac ctgtatgtgc
     9961 cttggtggct gtacggcaag gacaacatac gcaagctcgg cttcgcgcgc ggctatcata
    10021 tcgagtacgg ttgcgggcgc cgcatgccgg gtctcggcac cggtcagagg ctcgaggatt
    10081 tcaccggcgg cagctacggt gcgtccttca aggccgatgt gcgccgttac tacggctcgt
    10141 tcgtgggttt cgccggccgg ggagagatga tcccgaacca ggactgctac tgcgaaatcg
    10201 atcccaacag caaggatcga tacggaattc ccgtgttgcg atttcactgg ctgtggtccg
    10261 aacacgaaac acgccaggcc gcgcacatgc aacagacctt cgccgccatc atcgacgcca
    10321 tgggcggaca tgtccgcggc gccgtcgagc acgacggacg taaagccatc gagccgggcg
    10381 gtaagatcat tcacgaagtg ggtggaacca tcatgggtga ccggccgcag gattccgtca
    10441 ccaaccaatg gggcgagacg tgggatgttc cgaacctcgt ggtgacggat ggcggaacct
    10501 tttgctcgaa tgcggataag aatccgacgc tgacgatcat ggcgttggcg tggcgctgcg
    10561 cggatcatat gctcgagcgc ctcaagcgcc gggagatctg aattgaacca accgccgcga
    10621 atcgatcgac gcacgacgct caagtgggtg cttggcgccg ccgcgtcatt gccccttctc
    10681 atcaaggtgc gagcggcctc gcatggggct ggctatggca cggatccgaa tctcatgccg
    10741 ctgtatcgtc caggcgaact ctggcctctc accctgaaca tcggtgagcg gcgcagtgcc
    10801 gcggctttaa gcgacatcat tatcccggcc gatgacaact cacccagtgc ctcggcggtg
    10861 ggcgtcgtcg acttcatcga cgaatggatc agcgcgccgt atccagacca acggca
//
LOCUS       DASWZT010000422        17338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_17676, whole genome shotgun sequence.
ACCESSION   DASWZT010000422 DASWZT010000000
VERSION     DASWZT010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 17338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17338
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17676"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VGE92_12000"
     CDS             <1..335
                     /locus_tag="VGE92_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="HEY1314596.1"
                     /translation="SICRTLVEQTPTKIFFPNADANLHEYTQGFGLTEREFRLIKDQL
                     EPGSRMFLIKQAHHSVVCQLDLKGFDAELKVISGRAGEVERVQRLMSLRGPDPKAWLP
                     EFMAAGGR"
     gene            375..1091
                     /locus_tag="VGE92_12005"
     CDS             375..1091
                     /locus_tag="VGE92_12005"
                     /inference="COORDINATES: protein motif:HMM:NF019608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HEY1314597.1"
                     /translation="MPTPNARSVFLCSLLLFALIPPVRAQWAVIDAPAIVQLVQEVQT
                     MQQQLATARSQLQSAQSTLQAMTGDRGMARLLSGTPRNYLPSTWTQATGAAQGGGSGA
                     YAALAADVRSGVAANAVLSPQRMASMSPAEQQLIQAARQSNALQAALSHEALANASGR
                     FSAIQSLIAAISTAADQKASLDLQARIAAEMAMLQNEQTKLQVLSVAGQAQDVSLRQQ
                     LQERVIDGHGRFATRFQPAP"
     gene            1121..2143
                     /locus_tag="VGE92_12010"
     CDS             1121..2143
                     /locus_tag="VGE92_12010"
                     /inference="COORDINATES: protein motif:HMM:NF016491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HEY1314598.1"
                     /translation="MGFFATFWAWLNGQLAAYIGDNTVRLAAALEPAVVTLATVYVMA
                     WGYLHLTGKIDEPIVTGLKRIALIVLVLGVGLRLWMYNMVIVDTFYDAPSRLAAAVVG
                     STDPVATIDTIWESGGTVAGNLWEKGGVFRGDFGFYLAGAVVWCLMGVLCVYAMFLIA
                     LSSIALAVLLALGPLFIGMLFFDATKRLFVAWIMQLANYALITVLTVMVAALLLHVVQ
                     SYAAQTAARGAAILTVDALHMVLVAVLVFLVLRQVMPIAAGLAGGVAINSFGLTSRGA
                     GWGLRSAARLVTPALALAASFRRSGAKNDDDALRGAAVNSSREVQGTTDATTEALARS
                     WRDMGR"
     gene            2172..2327
                     /locus_tag="VGE92_12015"
     CDS             2172..2327
                     /locus_tag="VGE92_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314599.1"
                     /translation="MRLIVCFIPLLWGCSAPSVRCDAHLQPINAPAAAGTSSHPAPAA
                     APARRTR"
     gene            2324..3049
                     /locus_tag="VGE92_12020"
     CDS             2324..3049
                     /locus_tag="VGE92_12020"
                     /inference="COORDINATES: protein motif:HMM:NF016238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HEY1314600.1"
                     /translation="MITVPSIQGYFREAASWDADRNAQFRRSAHAAWWVAGAGWVCAV
                     ASSAALIVLTPLKSVDPFVVRVDNSTGIVDVVPVYTGLATPQEAVTRYFLTHYLSVCE
                     RFNFATAESDYEECGAFHSAQRNQAWSALWAASNPASPLNLHKDGSIVRVQVNSVSFF
                     TRSSGLTDLAQVRYLKAASQGVGSEESITHWIATIQYAYVDPSKDPKIRRWNPLGFKI
                     VDFRAETEVSVEQPAAKELHHEP"
     gene            3039..3869
                     /locus_tag="VGE92_12025"
     CDS             3039..3869
                     /locus_tag="VGE92_12025"
                     /inference="COORDINATES: protein motif:HMM:NF015487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HEY1314601.1"
                     /translation="MNPRASCRAVALLVSIAACLPGVAAFAETVPAKGSVDSRIRVAT
                     YNGDEVYRLRGYVGYQIDLEFEAGEIFTGLGAGDLEGLSFVGQDNHLFLKPKAAELAT
                     NLTVLTNRRHYHFDYSAMPERPAADDAGVIYSLRFTYPPLASESAAEAAAKRLDSQLA
                     RASAARQQNIDYWYCGEPAIKPLAASDDGVHTRLRFAANADLPAIFVHNEDGAESLLN
                     FSMEAGDVIVHRVARQLILRRGKLKGCIVNQGFRGGGTRLDSGTVAPTVERQVQGAAP
                     "
     gene            3866..5008
                     /gene="virB10"
                     /locus_tag="VGE92_12030"
     CDS             3866..5008
                     /gene="virB10"
                     /locus_tag="VGE92_12030"
                     /inference="COORDINATES: protein motif:HMM:NF015688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein VirB10"
                     /protein_id="HEY1314602.1"
                     /translation="MNEVDSAEGSVRGERGATAVNRTGSLQSRISSILAVGLMSVLGV
                     GMLTWYYAHAITRQSRERQSAQALSANRAQGDAALPSLGPIDAPLPPPQPAAPALLSP
                     TPLHEIPLEVSATPPASGVPAPKTAAQLRLERQLSGTVYSAQSAPTITAAASAGPDVR
                     VPPGAAQDPAELAALLRPSVTAAVHAQVLPTQRLLLAKGAFIDCTLETALDSTLPGMT
                     TCIMATDTFGVDGQVVLLERGTKLIGETRGQVQQGSARVFVLWTEARSPTGVIVPLAS
                     PGTDELGRSGLAGTVDNHFWQRFGAAMLVSVIDGAVEGAVQSARGGNSTVIVNPSAQG
                     VMTEVLKGTVNIPPTVRKQQGDRIQVLVARDLDFRSVYELRSVADR"
     gene            4980..6011
                     /gene="virB11"
                     /locus_tag="VGE92_12035"
     CDS             4980..6011
                     /gene="virB11"
                     /locus_tag="VGE92_12035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA];
                     GO_process: GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HEY1314603.1"
                     /translation="MSFDPLPIASAHEAESALGLTLRALRPLLGNADVMELCINRPNE
                     AFLETRDGWHREPLPFADFDWCSRLAKLVANSTQQRIDATSPLLSASLPSGERVQIVI
                     PPATTQGCVAIAIRRPAEQVWSIEELARRGLFCRTQRAAHKLEATEEELLRLLASADY
                     EAFMRLAVRSRKNILVSGPTGSGKTTCTKALIREIPRDERLITIEDASELVLDRHPNH
                     VRLYYSKDDQGIARVTPKQLLESCLRMKPDRILLAELRAEEAFDYLRNVNSGHPGSIT
                     SVHATSAELAFEQMVLLVKQNPGGRGLARQDIKSLLYLLIDVIIQFGVEHHQRFVKEI
                     WFDPARKHR"
     gene            6008..7651
                     /locus_tag="VGE92_12040"
     CDS             6008..7651
                     /locus_tag="VGE92_12040"
                     /inference="COORDINATES: protein motif:HMM:NF021897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HEY1314604.1"
                     /translation="MIDAAITQGWSGIALGYAAVQVPMVAALVRARAKLHQFVLSPVF
                     AVPLTAIAGVGIALSSSVLAHWGIAGDSATQLVFGAGCCAALGYAGGRKLARPAEDNS
                     HRRGAVVSNRAPACAKARSRDTEITLAGVAIPAQDETKHFKLIGTTGTGKSTAIREIM
                     TQALARGDRAVIADPDGGYMRRFYRPERGDVLLNPFDQRSVKWNLFAEIDSDYDVEQL
                     ARSLIPDHDGPDRSWRGYARTFFTAVTRQAHEAGIKDVSELYRLLVVAETAELRSLAR
                     GTPAQPFLDEHNSRMFDSIRSVTSSAVGALEYIGRQAAQDFSIRGWVREQGRGVLFIP
                     YKAGQIAALRSTISAWMRLAIFEAMEREDVQETAQTQGGRNSRLWFVVDELDALGQID
                     GLKDALARLRKFGGRCVLGFQSIAQVSSTYGQGDAHTIVENCGNTLILRCSASEGGGT
                     ARFASQLIGEREVMRTTVSKSRRLTELVGSITHAQHLNVEPAVLPSQVEQLPDLAGYL
                     KYASDPIWQRVRLDATSAWQQQRRDGGPEHRSHQGAERD"
     gene            7644..8159
                     /locus_tag="VGE92_12045"
     CDS             7644..8159
                     /locus_tag="VGE92_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314605.1"
                     /translation="MIDRVPMTDDATLKFGLLMESAQAHQKLAETHLQQLRAHTQDLD
                     GVVRDEIRRTLIEELQMLTAESQRAADSLRRIKRSANTRVALLSVALSIVCTAIPTAV
                     MGWLVPSKTEIAQLRAKRDELAANLTALERRGGRLEWRNCGEATRLCVRIDRKAPSYG
                     EKADFYVVAGY"
     gene            8140..9534
                     /locus_tag="VGE92_12050"
     CDS             8140..9534
                     /locus_tag="VGE92_12050"
                     /inference="COORDINATES: protein motif:HMM:NF021897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HEY1314606.1"
                     /translation="MWLLVIETLGSSAVVVAAAFMLVLVGCARLRRGPSQGTHRRGVV
                     LIDGRKAQRRARRRQSRAAELTLAGVAIASADETKHFKLLGTTGTGKSTAIRELLGAA
                     LERGDRAVFADPDGGYCSRFYRRYRGDIVLNPFETDSMKWDLFAEIHNSYDVEQLAGG
                     LIPAGDDASANEWRGYARTFLVAVVRRALACGWRDPANLWRLVTTASTEELRPILAGT
                     PAQPFLEPDNARMFGSIRAVAVSAVAAFEYIQAQRAAPFSVRAWVRAPSRAGILFIPY
                     KAGQIAALRSMIATWMRLAIFEAMSQPEEHDQRLWFVVDELDAIGRIDGLKDALARLR
                     KFGGRCVLGFQSIAQVSCTYGASEAQTIVENCGNTLILRCSGSENGGTSQFASRLIGD
                     REICRRQVSRGRDREGGWSTRGARRSQSVTEHYTTEPAVMPSELEQLPDLCGYLKRAS
                     SPQWLKIAFGGRHG"
     gene            9527..10024
                     /locus_tag="VGE92_12055"
     CDS             9527..10024
                     /locus_tag="VGE92_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314607.1"
                     /translation="MGDENVASAGHTVADANVRADTYFVVSTTARCWHCGTPTRLLAL
                     ALPNGHEVLEEHEWQYSSSSALLFYVEHVSAEVRDQLKEISSCFHPGYSVATLNTYWA
                     NHCEFCATLLDDHDLHCEPDSAFAMSSEAAAAHIELSHIEKPFAASTAGYSFDPEFFD
                     LARVS"
     gene            10031..11047
                     /locus_tag="VGE92_12060"
     CDS             10031..11047
                     /locus_tag="VGE92_12060"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HEY1314608.1"
                     /translation="MAVYYLNVKTFGRSGGSSAASAAAYRAGERIRDERTGRIYDHTD
                     RLDVMHKEIVLPSRFRDEAMAWARDRASLWNSAESAESRSNARVAREYLVALPVELSP
                     PERLGLARGFSQELSDRYSYALDLTIHAPRDFPGSDPRNFHAHLLATTREVLAQGLGA
                     KTTLELSGRDRNKLGLPPAINELLHVRERWATVTNEALREAHVAARVDHRSLEAQGID
                     REPKVYIPHAAFEMERQGYRSFVADRLRAEHQARVEERQQRSASREISQAAANAAAEP
                     QSLEEIRREARENWLRLRQSQGQGEDQSQSQSQSQSQSQIEGPDAGETSRPARGRGRD
                     GFSL"
     gene            11213..11734
                     /locus_tag="VGE92_12065"
     CDS             11213..11734
                     /locus_tag="VGE92_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314609.1"
                     /translation="MTDSALAIRLERALLEADGLLGAHCVHELWMRGEMSINIERALE
                     KLWARAAASVPEWLPMRYIEWLPIAYDIALGFRSNETGRSNIYLVLLDYQDRDGAYGV
                     YVGMSKYSPAQRFDQHKAGIRAAGSVLKRGIEVLTGPTLHLQYIKRSAAARIEAELAL
                     ALAAAGLRVMGGH"
     gene            11731..12372
                     /locus_tag="VGE92_12070"
     CDS             11731..12372
                     /locus_tag="VGE92_12070"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY1314610.1"
                     /translation="MILIGMFDSPFVRRVAVSMNLLGMVFEHRDWSVGKDFELIRQFN
                     PLGRVPTLVRPDGEALIESAAILDFLDECAGPGRALIPAMGKDRRDVLRILAVATGGA
                     DKGVTQIYETAFRPPEKRYRPWMERCNTQMHAALAELNRMCQARTGDWLIGNRMTQAD
                     VTATCVYTFLVDALAINRADVAYPGLAAIAARCEALPEFRSVKAEWFAPGQKG"
     gene            complement(12406..13443)
                     /gene="fbp"
                     /locus_tag="VGE92_12075"
     CDS             complement(12406..13443)
                     /gene="fbp"
                     /locus_tag="VGE92_12075"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF006778.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEY1314611.1"
                     /translation="MNTAGGPAPKSTRDDLRIMTLQAHILEQQVHYPQAGSTFSWILS
                     ALSISAKIIADKVRRARLENVLGSVGSENVQGEVQQKLDVLANEVLLRTLGGREGVAI
                     VASEENEEPVILREDSQGTPRYCVLFDPLDGSSNLDICGAVGTIFSILSHDGAQRAAH
                     SLLQPGMRQVAAGYVLYGSSTVFVLTLGKGVDLFVLDPSIGAFMRVEQNLRIPAANKT
                     YSVNEGNRQSFPEGYVRYLDWAQSNGYSSRYAGAMVADVHRILLKGGVFLYPPTAKSP
                     QGKLRLMYEANPMAMIVEQAGGRAVAAPGQRILEIQPTAIHQRTPVVLGAADQVDAVL
                     EQMKPKPLERA"
     gene            13471..14136
                     /locus_tag="VGE92_12080"
     CDS             13471..14136
                     /locus_tag="VGE92_12080"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY1314612.1"
                     /translation="MITVHHLNNSRSQRVLWLLEELGVAYEVKRYERDSKTMLAPPAL
                     LAIHPLGKSPVIVDGGVTVAESGAIIEYLLDHYGGGRLIPPVGTPERLRYTYWLHYAE
                     GSAMPPLLLKLVFDRVANGPAPWPISSVARRIAATVQSAFIGPQLKRHLDYMESQLAL
                     HSWFAGEEFTAADVQMSFPVEAAASRAGLDASRPRLMAFLERIHARPAYKRALERGGP
                     YAF"
     gene            14205..15968
                     /locus_tag="VGE92_12085"
     CDS             14205..15968
                     /locus_tag="VGE92_12085"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY1314613.1"
                     /translation="MLKSLHQAALSLILLAALAGCNQSPPASVAPKGPSLASQAWNKL
                     TAGFIESYLAAQPFFAAQAGRHEFDGQLPDLSEHGIRREIARLHDQRDQIAAVDPKTL
                     EPRERFDRDYLLAVVDRDLFWMEKAKFPFSNPSWYIDKIDPDMYLNRNYAPLTVRMKA
                     YIKYARGIPKMVNDIKANLQSPLPKTYLELGIAQYGGLSDFYSKNVAPTFASVTDPDL
                     QKQLSDADTAAAQAMDGLKQALVESLKKANDKYALGPDLYAEMVKQTESVEVPVAEIE
                     AAGKADLDRNTAALKAECDTFAPKVALMQCIAKVAAHKPKDGAVEAARAQLVMLKDFI
                     VKHNVVSIPSTDEAMVAEAPPYNRSNAAFIQVPGPYDRGVVSVFNIAPPDPKWSKAEQ
                     AAYVPSEANLLFTSVHEVWPGHFLQFLHSNSNPSKLEGLWIGYAYAEGWAHYCEEMMH
                     EEGLGKDDPEKHIGQLTDALLRDVRLLSSIGLHTHGMTVAQSEKMFREQAFQDPGNAR
                     QQAARGTYDPAYLNYTLGKLMIRKLRTDWIAKHAGANAGAAPADEHALWHDFHDQFLS
                     YGGPPIPLLRKEMLGESGAVL"
     gene            16107..>17338
                     /locus_tag="VGE92_12090"
     CDS             16107..>17338
                     /locus_tag="VGE92_12090"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1314614.1"
                     /translation="MNQLLAWMRAGRCAEVEASARTLSARFPDAGMVWKLLALSLTKQ
                     GKGALEALQKAAQLLPRDAEVHFHLGSALQLRGQLQAAIESYRRALAIDPGISQAHAN
                     LGLALQDLGQPEEALASYRRSLELAPDAAAAHTNLGNALRDLGRFDEAIKSQRRALHL
                     QPNLAEARINLGNVLQDVGQLNEATASYRHALELQPGLAAAHNGLGSALRRLGRIEDA
                     EASFRQAVLLNPRYAEGHTNLGIVLRLQGRTEEAEKCVRTALEINPSCAAAIQFSGEL
                     SADRGNFVEAEQRFRRAIEVTPSSIEAWAAIPGLRKMTSADAQWLAGAERMAARSLPQ
                     RQEIALRYAMGKYFDDVRQFTQAFANYRRANEVTQLCTPKYEPRQMAQTVSRTIDRYG
                     SAWMSPRGDSVAASRPVF"
BASE COUNT         3251 a         5311 c         5614 g         3162 t
ORIGIN      
        1 gttcaatctg ccgcaccttg gtcgagcaga cgcccaccaa gattttcttt cccaacgccg
       61 acgccaactt gcacgaatac acgcaagggt tcgggctgac cgaacgggaa tttcggctca
      121 tcaaggatca gctggagccg ggttcgcgaa tgtttctgat caagcaggcg catcacagcg
      181 tggtctgcca gctcgatctg aagggttttg atgcggagct caaagtgatc tcgggccgcg
      241 ccggcgaagt cgagcgcgtg cagcggctca tgtccctgcg cgggcccgat ccaaaggcgt
      301 ggttaccaga gttcatggcg gcgggcggcc gctgaccggt gttgtccaac gtactcgatc
      361 gaaaggagat gctcatgccg accccaaatg ctcgctcggt cttcctgtgc tcgctgctgt
      421 tgttcgccct cattccaccg gtgcgggcgc agtgggcggt catcgacgcg ccggcgatcg
      481 tgcaactggt gcaggaagtg cagaccatgc agcagcaatt ggcgactgcg cgctcgcagc
      541 tgcaatccgc gcagtccacg ctgcaggcga tgaccggcga tcgcggcatg gcgcggttat
      601 tgagcggcac cccgcgcaac tacctgcctt ccacttggac ccaggcgacc ggggccgcgc
      661 agggcggcgg aagcggtgca tatgccgcgc tcgccgccga cgtgcgcagc ggcgtagccg
      721 cgaacgccgt attgtcgccg cagcgcatgg catcgatgtc gccggccgag cagcagctaa
      781 ttcaagccgc gcgccaatcg aatgcgctgc aagccgcgct ctctcacgag gcgctcgcga
      841 atgccagcgg ccggttctcg gccattcaaa gcctcatcgc cgccatatcg acggcagccg
      901 atcagaaagc cagcctcgac ttgcaggcgc gcatcgctgc cgaaatggcg atgttgcaaa
      961 atgaacagac caagctgcag gttctctccg tggccggcca agcgcaggac gtctctctgc
     1021 gacagcagct gcaagagcgc gtgatcgacg ggcacgggcg tttcgccacg cgcttccagc
     1081 cggcaccgtg agcctcgctt aagcgcaccg gggtactccg atgggattct tcgcgacatt
     1141 ttgggcatgg ctcaacggcc aactggccgc ctacatcggc gacaacaccg tacgtctcgc
     1201 cgcggcgctc gagccggccg tcgtcacgct ggcgacggtc tacgtgatgg cgtggggcta
     1261 cctgcacctc accggcaaga tcgacgaacc gatcgtgacg ggcctgaaac gcatcgccct
     1321 gatcgtgctg gtgctgggag tggggctgcg cctctggatg tacaacatgg tgatcgtcga
     1381 caccttttac gatgcgccgt cgcggctcgc cgccgcggtg gtgggcagca cggatccggt
     1441 cgcaaccatc gataccatct gggagagcgg cggcacggtg gcgggcaatc tgtgggagaa
     1501 gggtggtgtt tttcgaggcg actttggctt ctacctcgcc ggcgcggtgg tttggtgcct
     1561 gatgggcgta ctttgcgtgt acgccatgtt tctgatcgca ttgtcgagca tcgcgctcgc
     1621 cgtgctgttg gcgctcggac cgctgttcat cggcatgctg ttcttcgatg ccacgaagcg
     1681 cttattcgtt gcatggatca tgcagctggc caactacgcc ttgataaccg tgctgaccgt
     1741 gatggtcgcc gctctgctgc tgcacgtcgt gcaatcgtac gccgcgcaaa ccgcagcgcg
     1801 cggtgcggcg attctgaccg tcgatgcgtt gcacatggtg ctggttgccg tgctcgtgtt
     1861 cctggtgttg cggcaggtca tgccgatcgc cgcgggcttg gccgggggcg tggccatcaa
     1921 ttcattcggc ctcaccagcc gcggggcagg ttgggggctg cgcagcgccg cccggctggt
     1981 aactcctgca ttagccctcg cggcatcgtt tcgcaggagc ggtgccaaga atgacgatga
     2041 cgcattgcgg ggggcggctg tgaattcgag ccgcgaagtt caaggaacta ccgacgccac
     2101 aacggaggcg ttggcccgaa gctggcgcga tatgggccgc tgagtcgaat tgcatcaagg
     2161 aggatgaagt catgcgtctg atcgtttgtt tcataccgct gctgtgggga tgttctgcgc
     2221 cgagcgtgcg ctgtgatgcg cacctgcagc cgatcaatgc ccccgcggcg gccggcacct
     2281 cgagccatcc ggcgcccgcg gcggcaccgg cgcggagaac gcgttgatca cggtcccgag
     2341 catccagggg tactttcgcg aagccgcgtc ttgggacgcg gaccgcaacg cgcaatttcg
     2401 ccgcagcgca cacgccgcgt ggtgggttgc cggtgccggg tgggtgtgcg cggtcgccag
     2461 cagcgccgcg ctcatcgtgc tgacgccttt aaaaagcgtc gatccgttcg tggtgagggt
     2521 ggacaacagc accggcatcg tcgatgtcgt gccggtctat acgggccttg cgaccccgca
     2581 agaggcggtg actcgctact tcctgacaca ttacttgagc gtgtgcgagc gcttcaactt
     2641 cgccaccgcg gaaagcgact acgaggaatg cggcgcattc cattcggcgc agcgcaatca
     2701 agcctggtcc gcgctgtggg ccgccagtaa tccggcatcc cctctcaatc tgcacaagga
     2761 cggcagtatc gtgcgcgtgc aagtcaattc cgtgagcttt tttacgcgat ccagcggcct
     2821 caccgatctc gcgcaagtgc gctacctcaa ggccgcgagc caaggcgtcg gatccgaaga
     2881 gtcgatcact cactggatcg cgaccatcca gtacgcgtat gtcgaccctt cgaaggatcc
     2941 gaagattcgt cgctggaatc cgctcggctt caagatcgtg gattttcgag ctgaaaccga
     3001 agtttcggtc gaacaaccgg ccgcaaagga gctacatcat gaaccctagg gcgtcgtgtc
     3061 gcgcagtggc cttgctcgtc tcaatcgcgg catgcctccc cggtgtcgcc gcgtttgcgg
     3121 aaaccgtgcc ggcgaagggc agcgtcgata gcagaatcag ggtggcaacc tacaacggcg
     3181 atgaggtcta ccgactgcgc ggttacgtcg gctatcaaat tgatctcgag ttcgaggccg
     3241 gcgagatttt caccggcctc ggagccggcg atttggaagg actgtccttc gtcggtcagg
     3301 acaatcactt gttcctcaag ccaaaggccg ctgagctcgc gaccaacctg accgttctga
     3361 ccaaccggcg gcactatcac ttcgactata gcgccatgcc ggagcgaccc gcggccgatg
     3421 atgcgggtgt gatttattcg ctgcgcttca cctacccgcc gctggcgtcg gaatccgccg
     3481 ccgaggcggc tgccaagcgc ctcgattcgc agctcgcgcg cgcctcggcc gcgcgccaac
     3541 agaacatcga ctattggtac tgcggcgagc cagccatcaa gccgctcgcc gcctcggacg
     3601 acggggtcca cacgaggctg cgctttgcgg cgaacgccga tctacccgcg attttcgtgc
     3661 ataacgaaga cggtgccgaa tcgcttctga atttcagcat ggaggcaggc gatgtgatcg
     3721 tgcatcgggt cgcgaggcag ttgatcctac gtcgcggcaa gttgaagggc tgcatcgtca
     3781 atcagggatt tcgcggtggc gggacccgtt tggattcggg aaccgtggcg ccgaccgtgg
     3841 agcgccaggt tcaaggagcc gcgccatgaa cgaagtcgat tccgcggaag gcagcgtacg
     3901 cggcgagcgc ggtgcgaccg cagtcaatcg cacgggatcg ctccagtcgc gcatcagcag
     3961 catcctggcg gtaggcctga tgagcgtgct cggcgtgggc atgctcacct ggtattacgc
     4021 gcacgcgatc acccggcaga gccgcgagcg gcagagcgct caagcattat cggctaaccg
     4081 cgctcaaggc gatgcggcgt tgccgagctt aggtccgatc gacgcgccgc tgccgccgcc
     4141 gcaacccgcg gcgccggctt tattgagccc gacaccgctg catgaaattc ccttggaggt
     4201 ttccgcaacg ccgccggctt ccggtgtgcc cgcgccgaag accgccgcgc aattgaggtt
     4261 ggagcggcaa ttgtcgggca ccgtgtattc ggcgcaaagt gcgccgacca ttaccgctgc
     4321 ggcgtcggcg ggacccgatg ttcgcgtgcc gccgggcgct gcgcaagatc ccgctgaatt
     4381 ggctgcgctg ctgcgcccaa gtgtcacggc cgcggttcac gctcaagttt tgcccacgca
     4441 acggctgttg cttgccaagg gcgcgttcat cgattgcacc ctggaaactg ctctcgattc
     4501 gaccctgccg gggatgacca cctgcatcat ggcgaccgac accttcggtg tcgacgggca
     4561 ggttgtgctg ctcgagcgcg gcaccaagct gatcggcgaa acacgcgggc aggtgcaaca
     4621 ggggtcggcc cgtgtgttcg tgctttggac ggaagcgcga tctccaacgg gagtaatcgt
     4681 gccgctggca tcgcccggta ccgacgagtt gggccgctcg ggacttgcgg gcacggtcga
     4741 caaccacttc tggcaacgct tcggcgcggc catgctggtg tcggtgatcg atggcgcggt
     4801 cgaaggcgcc gtgcagtctg cgcgcggcgg caacagtacg gtgatcgtca atccgtccgc
     4861 ccagggcgtc atgaccgaag tactcaaggg caccgtcaat atcccgccca cggttcgcaa
     4921 acagcagggc gatcgcattc aggtgttggt ggcgcgcgac ttggatttca gatcggttta
     4981 tgagcttcga tccgttgccg atcgctagcg cacacgaggc agagtccgcg ttgggcttga
     5041 ccttgcgtgc acttcgtccg ttgctcggga atgccgacgt gatggaactt tgcatcaatc
     5101 ggcccaacga agcctttctt gaaacacgtg acggctggca tcgcgagccg ctgccgttcg
     5161 ccgatttcga ttggtgtagc cggctggcga agttggtcgc caattcgact cagcagcgca
     5221 tcgatgcgac atcgcccttg ctgtcggcct ctctgccgag cggcgagcgg gtacagatcg
     5281 tcattccccc ggccaccaca caaggctgcg tggccatcgc gatccggcgt ccggccgagc
     5341 aggtgtggag catcgaggaa ttggcgcgtc gcggactctt ttgtcgaacg cagcgggcgg
     5401 cgcacaagct cgaggccacc gaggaagagc tgctgcgatt gctggcgtcg gccgactatg
     5461 aggccttcat gcgccttgcg gtgcgcagcc gcaaaaacat cctggtgtcc gggcccaccg
     5521 gctccgggaa aaccacctgc accaaggcgc tgatccgcga gatcccccgc gacgagcggc
     5581 tcattaccat cgaggatgcg agcgagttgg tgctggaccg tcacccaaat cacgtgcggc
     5641 tgtactattc gaaggacgat caaggcattg cgcgcgtgac gcccaagcag ctgctggagt
     5701 catgcctgcg catgaaaccg gaccggatcc tgctcgcgga actgcgggcg gaagaggcct
     5761 tcgactattt gcgaaacgtc aattccggcc atcccggctc catcaccagc gtgcacgcga
     5821 ccagtgcgga attggctttc gagcagatgg tgcttctggt caagcagaac ccgggcgggc
     5881 gcgggcttgc gcgccaggac atcaagagcc tcctgtacct cctgatcgat gtgatcatcc
     5941 aattcggcgt cgagcatcac cagcgcttcg tcaaagagat ctggttcgac ccggcgcgca
     6001 aacaccgatg atcgacgcgg caatcacgca gggatggtcc ggcattgcct taggatatgc
     6061 cgcggtgcag gtgcccatgg ttgcggcatt ggtacgcgcc cgcgcaaaac tccatcaatt
     6121 cgtgctctcg cccgtgtttg cggtgccatt gacggcgatc gcgggcgtcg gtattgcgct
     6181 gtcgagttca gtattggctc actggggcat tgccggcgac agtgccacgc agttggtgtt
     6241 cggagcgggc tgttgtgcgg cgctcggcta cgccggcggc aggaagctcg cgcgcccggc
     6301 cgaggacaat tctcaccggc gtggtgccgt ggtcagcaac cgcgcgccgg cctgcgcgaa
     6361 ggctcggtcg cgcgataccg aaatcaccct ggcgggcgtg gccattccgg cgcaggacga
     6421 gaccaagcat ttcaagttga tcggtaccac cggcaccggc aagagcacag cgatccgaga
     6481 aatcatgacc caggcgttgg cgcgtggcga ccgggccgtg atcgccgatc cggatggcgg
     6541 ctacatgcgg cgattctacc ggcctgagcg cggcgatgta cttctcaatc ccttcgatca
     6601 gcggtcggtg aagtggaatc tgttcgcgga aatcgacagc gattacgacg tcgaacaact
     6661 cgcgcgatcg ttgattccgg atcacgacgg accggaccgc agctggcgag gctatgcacg
     6721 cacgttcttt acggcggtca cccgccaggc gcatgaggca ggaatcaagg acgtgagcga
     6781 gctctaccgg ctcttggtcg tggcagagac cgcggaattg cgcagtctcg cgcggggcac
     6841 gccggcacaa ccgttcttgg acgagcacaa cagtcgaatg ttcgactcga ttcgctccgt
     6901 gacgagttcc gcggtgggag cactcgagta catcggacgc caggcagcgc aagatttctc
     6961 gatccgcgga tgggttcgcg agcagggccg gggcgtgctg ttcatccctt acaaagcagg
     7021 tcagatagcg gctttgcgtt cgactatttc ggcgtggatg cgcctcgcga ttttcgaggc
     7081 tatggaacgg gaggacgtgc aggagacggc acagacgcaa ggcgggcgca acagccggtt
     7141 atggttcgtc gtcgatgaat tggatgcctt ggggcaaatc gatggcctga aggacgcgct
     7201 cgcgcggctg cgtaagtttg gcggccgctg cgtgctggga ttccagtcaa ttgcgcaagt
     7261 gtcgagcact tacggccagg gcgacgcgca cacgatcgtc gagaattgcg gcaacacctt
     7321 gatcctgcgc tgttcggcca gcgaaggcgg tggcaccgcg cgtttcgcct cgcaactcat
     7381 cggcgagcgc gaggtcatgc gcacgacggt gtccaaaagc cgccgcctga ccgaactcgt
     7441 gggctcgatc acccacgctc agcatctcaa cgtggaaccc gcggtattgc cgtcgcaagt
     7501 cgagcagctt cctgacctcg cgggatattt gaaatatgcc tcggatccga tctggcagcg
     7561 cgtgcggctc gacgccacgt ctgcgtggca acagcagcgg cgcgacggcg gtcctgagca
     7621 ccgcagccat cagggagccg aacgtgattg atcgcgttcc gatgacggac gacgcgacgc
     7681 tgaaatttgg tctgctcatg gagagtgcgc aggcgcatca aaaactcgcc gaaacccatc
     7741 tgcaacaatt gcgggctcat acgcaggacc tcgacggcgt cgtacgcgac gaaatacgac
     7801 gcacgctcat tgaggagctg caaatgctga cggcggagag ccagcgtgcc gccgacagct
     7861 tgcgacgcat caagcgaagt gccaacacgc gggtcgccct cttgagcgtt gcgctgtcaa
     7921 tcgtctgcac cgccattcca acggccgtca tgggctggct tgtaccctcc aaaaccgaga
     7981 tcgcgcagtt gcgcgccaag cgcgacgaac tggcggcgaa ccttaccgcc ctcgaacggc
     8041 gcggcggccg gctcgagtgg cggaattgtg gcgaggcgac tcggctttgc gtgcggattg
     8101 accgcaaggc gccgagctat ggcgagaagg cggatttcta tgtggttgct ggttattgaa
     8161 accttggggt catcggcggt cgtggtggct gccgctttca tgctcgtgct ggtcgggtgc
     8221 gcgcggctgc ggcgcggacc atcgcaaggc acgcaccggc gcggcgtggt attgatcgac
     8281 ggacgcaaag cgcagcgccg cgcccgtcgg cgacaatcgc gcgccgccga gttgacgctg
     8341 gcgggagtcg ccatcgcgtc agcggacgaa accaagcact tcaagctgct tggcaccacc
     8401 ggaaccggta agtcgacagc gatccgcgag ctcctcgggg cggcgctcga gcgcggcgac
     8461 cgcgctgttt tcgccgaccc cgacggcggc tattgctcgc gattttatcg gcgttatcgc
     8521 ggcgacatcg tattgaatcc attcgaaaca gattcgatga agtgggatct gttcgccgag
     8581 attcacaaca gctacgacgt cgagcaactc gccggcggcc tgatacccgc cggcgacgat
     8641 gcgtcggcca acgagtggcg gggctacgcc cggacttttc tggtcgcggt ggtgcgccgc
     8701 gcgcttgcct gcggttggcg cgatcccgcc aatctttggc ggttggtaac taccgcctcg
     8761 accgaggagc tgcggccgat tctcgcgggg acgccggcgc agcctttcct cgaacccgat
     8821 aacgcgcgca tgttcggctc gattcgagcg gtggcggtct cggccgtggc cgcgttcgag
     8881 tacatccaag cgcagcgggc tgcgcccttc tcggtacgcg cctgggtgag agcgccgagc
     8941 cgtgcgggga tcctattcat accgtacaag gccggccaaa tcgcggcgct gcgctcgatg
     9001 atcgccacct ggatgcgtct tgcgatattc gaggccatga gccaaccgga agagcacgat
     9061 caacggctct ggttcgtcgt cgacgaactc gacgcgatcg gccgtatcga tgggctcaaa
     9121 gacgccttgg cgcggctgcg caagtttggc ggccgctgcg tgctgggatt ccagtcaatt
     9181 gcgcaggtgt cctgcaccta tggggcaagt gaagcgcaga cgatcgtcga gaactgcggc
     9241 aacacgctca tcctgcgctg ctccggcagt gaaaacggcg gcacgtcgca attcgcctcc
     9301 cggctgatcg gcgatcggga aatctgtaga cgccaagtgt ctcgcggcag ggatcgggag
     9361 ggcggctggt cgacgcgcgg cgcgcgtcgc tcgcaaagcg tcaccgagca ttacacgact
     9421 gaacccgccg tcatgccctc ggaactggag cagttaccgg atctgtgcgg ctacctcaag
     9481 cgcgcctcct cgccgcagtg gttgaagatc gcattcggag gccgccatgg gtgatgagaa
     9541 cgtcgcgtcc gctggacaca ccgtagcgga cgccaatgtg cgagcggaca cctatttcgt
     9601 ggtcagcacc acggcacgct gttggcactg cggcacgcca acccggctgc tggcgcttgc
     9661 gttgccgaac ggccatgaag ttctcgagga gcacgaatgg cagtattcga gctcgagtgc
     9721 gcttctcttc tacgtggagc atgtgtccgc ggaagtacgc gaccagttga aggagatctc
     9781 gagttgcttt cacccgggat acagcgtagc gacgttgaat acctactggg cgaatcattg
     9841 cgaattctgc gcaacgctgc tggatgatca cgacctgcat tgtgagccgg atagcgcatt
     9901 cgccatgtcg agcgaagcgg cggcggcgca tatcgagctg tcgcatatag aaaaaccctt
     9961 cgcggcatcg accgccggat attcgttcga ccccgaattc ttcgatcttg ctcgcgtgag
    10021 ctaacgcgtc gtggcggtct actacttgaa cgtcaagacg ttcggtcgct cgggcggcag
    10081 cagtgccgcc agcgcggcgg cctatcgggc gggcgagcgc atccgcgatg aacgcacggg
    10141 caggatctat gaccataccg accggctcga cgtcatgcac aaggaaatcg tgctgccgag
    10201 ccgattcagg gacgaagcaa tggcatgggc ccgggatcgg gccagcctgt ggaattcggc
    10261 ggaaagcgca gaatcacgca gcaacgcccg ggtcgctcgc gagtatctcg tcgcgctccc
    10321 ggtggagctc tcgccgccgg agcgtttggg gctcgcgcgc ggattctcgc aagagctgtc
    10381 ggatcgatac agctatgcgc tcgacttgac catccatgcg ccgcgggatt ttccgggcag
    10441 cgatccgcgc aattttcatg cacacctgct ggcgacgacc cgcgaggtac tcgcccaagg
    10501 tctgggggcg aagacgacgc tggaattgag cggccgcgac cgcaacaagc tcggcctgcc
    10561 gcccgcgatc aacgaactcc tgcatgtgcg cgaacggtgg gcaacggtca ccaacgaagc
    10621 gctgcgcgag gctcatgtcg cggcgcgcgt cgatcatcgc agtctcgagg cgcaggggat
    10681 cgaccgcgaa cccaaggtgt acattccgca tgccgccttc gagatggaaa gacagggata
    10741 ccgcagcttc gtagcggatc gcctgcgtgc agaacatcag gcacgcgtcg aggagcgtca
    10801 gcagcgctct gccagtcgcg agatctcgca agcggcggcg aacgcggccg ccgaaccgca
    10861 gagtctcgag gagattaggc gagaagctcg cgagaattgg ctgcggctac ggcaaagcca
    10921 gggccagggc gaggaccaga gccagagcca gagccagagc cagagccaga gtcaaatcga
    10981 gggtccggac gcgggcgaaa cttcgcgccc ggcccgaggg cgtggtcgcg acggcttcag
    11041 cctatgagac gcgcgatgac gcttgctgcg taacgcatgc cggtgagcgg atttgcctaa
    11101 atcgctggcg tgtcatatat cagatcgacg atcgatcagg agccggtgac ggtgctgcga
    11161 attgccgcgc gaaaaactca atcttccacc tggtagtcgc ccgcttcagg gtttgaccga
    11221 cagcgccctt gcgattcgcc tggagcgcgc tttgctggag gcggatggtt tgctgggcgc
    11281 tcactgcgtg cacgagctgt ggatgcgtgg cgaaatgtcc attaacatcg aacgagcgct
    11341 ggaaaagctt tgggcacgcg ccgcggcgtc cgtacccgag tggcttccga tgcgctatat
    11401 cgagtggctc cccatcgcgt acgacatcgc gctcgggttt cgctccaatg aaacgggccg
    11461 atcgaatatt tacttggtac tgctcgacta tcaggaccgt gatggggcat atggcgtgta
    11521 cgtcggtatg agcaagtaca gtcccgcgca gcgcttcgac cagcacaagg cggggattcg
    11581 cgcggccggc agcgttttga agcgcggtat cgaagtgctc acgggtccga ctctgcattt
    11641 gcaatacatc aagcgatccg cggcggcgcg aatcgaagcg gagctcgcac tagcgctggc
    11701 tgcggccggc cttcgggtca tggggggaca ttgattctca tcggcatgtt cgattcgccc
    11761 ttcgtgcgtc gcgtcgccgt cagcatgaat ctgctcggta tggtgttcga acatcgcgat
    11821 tggtcggtcg gtaaggactt cgaattgatc cgccaattca accctctcgg ccgcgtgccc
    11881 accttggtac ggcctgacgg cgaggcactg attgaatcgg ccgcgatact ggattttttg
    11941 gatgaatgtg ccggacccgg gcgcgcactg ataccggcca tgggcaagga tcggcgcgat
    12001 gtgctgcgga tattggcggt cgctaccggc ggcgccgaca agggtgtgac gcaaatttac
    12061 gagaccgcgt ttcgcccgcc cgaaaagcgc taccgcccgt ggatggaacg ctgcaacacg
    12121 cagatgcacg cggcgctggc tgagctcaac cgcatgtgcc aggcccggac cggggactgg
    12181 ttgatcggta atcgaatgac tcaggcggat gtgactgcaa cctgcgtgta cacgttcttg
    12241 gtggatgcgc tggcaatcaa ccgcgctgac gttgcctatc cggggctcgc cgcaatcgcc
    12301 gctcgctgcg aggcattgcc ggaatttcgt tctgtcaagg ccgaatggtt cgcgcccggc
    12361 cagaagggct gaatagcacc gccggaaact ccgaccgcgg caggctcagg cccgctccaa
    12421 tggcttcggc ttcatttgct cgagcaccgc atccacctgg tccgctgcgc cgagaacgac
    12481 cggggtgcgt tgatgaattg cggtcggttg aatctcgagt atccgttgcc cgggggccgc
    12541 gacggcacgg ccgccggcct gctcgacgat catggccatc ggattggctt cgtacatgag
    12601 ccgcagcttg ccttgcggtg atttggccgt cggcggatag agaaacacgc cgcccttgag
    12661 caagattcga tgcacgtcgg cgaccatcgc gcccgcgtag cggctcgagt aaccgttgct
    12721 ctgcgcccaa tcgaggtagc gcacatagcc ttccgggaag ctctggcgat tgccctcgtt
    12781 gaccgaatag gtcttattgg ctgcgggaat ccgtagattc tgttcgaccc gcatgaaggc
    12841 cccgatgctc ggatcgagca cgaacaggtc cacgcccttt cccaaggtga ggacgaacac
    12901 cgtggacgag ccgtacaaaa cgtagcccgc cgcgacttgc cgcatgcccg gctgcagcag
    12961 cgagtgggcg gcgcgctgcg cgccgtcatg cgacaaaata ctgaagatcg tgccgacggc
    13021 gccgcagatg tcgagattcg aagaaccgtc gaggggatcg aacagaacgc agtagcgcgg
    13081 cgttccttgg ctgtcctcgc gcaaaatcac cggttcttcg ttctcttccg acgcgacgat
    13141 ggcgacgccc tcgcggcctc ccaaggtgcg caacagcact tcattggcga gcacatcgag
    13201 cttttgctgg acttcgccct ggacgttttc gctgccgacg ctgccgagaa cgttctccag
    13261 acgcgcgcgg cgcactttgt cggcgatgat cttcgcggaa atcgacagtg cggacaggat
    13321 ccatgaaaac gtgctgcccg cctgcggata gtggacctgc tgctcgagga tgtgcgcttg
    13381 cagcgtcatg atgcgcaagt cgtcgcgagt ggatttgggt gcggggccgc ctgcggtgtt
    13441 cacagccgaa gaatgccata caataccgca atgatcaccg tgcaccacct gaacaactcg
    13501 cggtcgcaac gggtgttgtg gctgctcgag gagttgggcg tcgcttatga ggtgaagcgt
    13561 tacgagcggg attctaagac catgctcgcc ccgccggcgc tgctcgcgat acaccccttg
    13621 ggcaagtctc cggtcatcgt ggacggcggc gtgacggtgg ccgagtcggg tgcgatcatc
    13681 gaatatctcc tggatcacta cggcggcgga cgcttgatac cgcccgtggg caccccggaa
    13741 cggctgcgat atacctactg gctgcactat gcggaagggt cggccatgcc gccgctattg
    13801 ctgaaattgg tgttcgatcg cgtggcgaac ggcccggcgc catggccgat cagcagtgtc
    13861 gcgcggcgca ttgccgcaac ggtgcaaagc gccttcatcg gcccgcagct gaagcgtcat
    13921 ttggactata tggagtcgca gctcgcgctc catagctggt tcgccggcga ggagttcacg
    13981 gcggcggacg tgcaaatgag cttcccggtc gaggccgcgg cgtcgcgcgc cggattggac
    14041 gcgagccgcc cgcggctgat ggcctttctc gagcgcattc acgcgcgccc tgcctacaag
    14101 cgcgcgctgg agcgcggcgg tccctacgct ttctaacggc acgccgcggc aacatctgag
    14161 gatggttgaa tcgaattctg atagcagtaa agaggggagt acacatgctt aaatcgctgc
    14221 accaagcggc cttgtcgctg attttgttgg ccgccctggc ggggtgtaat cagtcgccgc
    14281 cggcatcggt cgcgccgaag gggccgtctc tcgcgagtca ggcgtggaac aagctcaccg
    14341 ccggcttcat cgaaagctat ctcgcggctc agccgttttt cgcggcgcag gcaggccgcc
    14401 acgagttcga cggtcagctg cccgatctca gcgaacacgg cattcgacgc gaaatcgcac
    14461 gcttgcacga tcagcgcgac cagatcgccg cggtcgatcc caagaccttg gaaccgcgcg
    14521 aacgcttcga tcgagactac ttgctggcag tggtggaccg ggacttgttc tggatggaaa
    14581 aagcgaaatt cccattcagc aatccgagct ggtacatcga caaaatcgat ccggatatgt
    14641 atctgaaccg caactatgcg ccgctcaccg tgcgcatgaa ggcctacatc aagtatgcgc
    14701 gcggcattcc gaagatggtg aatgacatca aagcgaatct gcaatcgccg ctgccgaaaa
    14761 cctacctgga actcggcatc gcacagtacg gcgggctgtc ggacttttat tcgaagaacg
    14821 tggcgccgac cttcgcctcg gtgaccgacc cggatctgca gaagcagctg agcgatgcgg
    14881 acaccgctgc ggcgcaggcc atggatggac tcaagcaggc gttggtcgag tctctcaaga
    14941 aggcgaacga caaatacgcg ctgggcccgg acctgtatgc agaaatggtc aagcagaccg
    15001 agagcgtcga agtgcccgtc gctgagatcg aagccgccgg caaggccgat ctcgaccgca
    15061 atacggcagc gctcaaagcg gagtgcgaca cgtttgcgcc gaaggtcgct ctgatgcaat
    15121 gcatagcgaa agtggcggct cacaaaccga aagacggcgc ggtcgaggcg gcgcgcgcgc
    15181 aactggtcat gctgaaggac ttcatcgtca agcacaacgt ggtctcgata ccgagcaccg
    15241 atgaagccat ggtcgccgaa gcgccgccct acaatcgctc caatgccgcg ttcatccagg
    15301 ttcccgggcc ctacgaccgc ggagtggtct cggtgttcaa catcgcgccg ccggatccga
    15361 agtggagcaa agccgaacaa gcggcatacg tgccgagcga agcaaatctg ctgttcacct
    15421 ccgtgcatga agtgtggcct gggcatttcc tgcagttttt gcattcgaac tccaatcctt
    15481 ccaagctcga aggcctctgg atcgggtatg cgtacgcgga aggttgggcg cattactgtg
    15541 aagaaatgat gcatgaggaa ggcttgggga aagacgatcc ggaaaagcac atcgggcagc
    15601 tcaccgatgc gctgctgcgc gacgtgcgcc tattgtcctc gatcggactg cacacgcacg
    15661 gcatgacggt ggcgcaatcc gagaaaatgt ttagagagca ggctttccaa gatccgggca
    15721 acgcgcgcca gcaggcggca cgcggtacct acgatccggc gtacctcaat tacactctcg
    15781 gcaaactcat gatcagaaag ctccggaccg actggatcgc caaacatgca ggcgccaacg
    15841 ccggcgcggc gcccgcggac gagcacgcgc tgtggcatga tttccacgac cagtttctct
    15901 cctacggcgg tccgccgatt ccgctgttgc gcaaggaaat gctgggggag tcaggcgccg
    15961 ttctatagga cgttcgccga tgagagaccc agagaaatcg cgtgaaaatc ggaaggaacg
    16021 acgcctgccc ctgcggtagc ggaaaaaaat acaagcactg ttgcgggcaa gttctcgcgc
    16081 aatccacagc gcccgcgcag ggcgaggtga atcagttgtt ggcgtggatg cgcgccggcc
    16141 gctgcgcaga ggtggaagcg agcgcgcgaa ctctgagcgc gcgattcccg gacgcgggga
    16201 tggtgtggaa gctgttggcc ttgtcgctga cgaagcaggg caaaggggcc ctcgaggctc
    16261 tgcaaaaggc cgcgcagctc ttgccacgcg acgccgaggt ccactttcat ttgggcagtg
    16321 cgttgcaact gcgtggacag ctgcaagccg cgatcgagag ctatcgccgc gcgctggcaa
    16381 tcgacccggg aatttcgcag gcacacgcca atttaggact cgcactacaa gacttgggac
    16441 agcccgagga ggcattggcg agttatcgac gctcgctcga gctcgcgccc gatgcggcgg
    16501 ccgcgcacac caatctcgga aacgcgttgc gggatctggg tcgatttgac gaggccatca
    16561 agagccaaag acgcgcgctc caccttcagc ccaacctcgc cgaggcacga atcaatttag
    16621 gcaacgtgtt gcaagacgtg gggcagctca acgaggcaac cgcgagctac cgccacgcgc
    16681 tcgagctgca acccggtctc gcggccgcgc acaacggctt aggaagcgcg ctacggcgtc
    16741 tcggcagaat cgaggatgcc gaagcgagct ttcgtcaggc cgtattgctc aatccgcgct
    16801 acgccgaggg ccacaccaat ctcggtatcg tcttgcggct gcaaggccgc accgaggaag
    16861 ccgagaaatg tgtccgcacg gcgctcgaaa tcaatcccag ctgcgctgcg gcgatccagt
    16921 tctcggggga gctctcggcc gaccggggaa attttgttga agcggaacag cgattcagac
    16981 gcgccatcga ggtcactccg tcgtcgatcg aggcatgggc cgccatcccg gggctgcgca
    17041 aaatgacatc ggccgatgcg cagtggttgg cgggtgccga gcgcatggcg gcgcgctccc
    17101 tgccgcagcg ccaggagatt gctctgcgct acgcgatggg caaatacttc gatgacgtgc
    17161 gccagttcac ccaggcgttc gccaactacc ggcgcgccaa cgaagtgacg cagctgtgca
    17221 cgcccaaata tgaaccccgg cagatggcgc agaccgtgag ccgcacgatc gatcgatacg
    17281 gctcggcttg gatgagcccg cgcggcgact ccgtggccgc gtcgcgaccg gtattcat
//
LOCUS       DASWZT010000423         5362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_56585, whole genome shotgun sequence.
ACCESSION   DASWZT010000423 DASWZT010000000
VERSION     DASWZT010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5362
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56585"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VGE92_12095"
     CDS             complement(<1..182)
                     /locus_tag="VGE92_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314615.1"
                     /translation="MKQLGTLINRRAMACGFLMAALGLCGCEHPPVETLQGGYRGVGM
                     EQIINPRIDAVRAGKNA"
     gene            complement(179..1153)
                     /gene="pufM"
                     /locus_tag="VGE92_12100"
     CDS             complement(179..1153)
                     /gene="pufM"
                     /locus_tag="VGE92_12100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009521 - photosystem [Evidence IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="photosynthetic reaction center subunit M"
                     /protein_id="HEY1314616.1"
                     /translation="MAEYQNIFTRVQIRAPAYVGVALPRGAWGRQGKGGFSHLLGRFG
                     DAQIGPIYLGTTGLLSLICGFIAFEIIGLNMFASVHWNPVQFIRQLPWLALEPPSPAN
                     GLHFPRLQDGGWWLMAGFFLTSSVLLWWLRMYRRARALGMGTHVSWAFLSAIWLMLVL
                     GIIRPVLMGSFGEAVPFGIFPHLDWTAAFSIRYGNLFYNPFHCLSIAFLYGSTLLFAM
                     HGGTILAVSRLGGDRELEQIVDRGTAAERAALFWRWTMGFNATMESIHRWAWWFAVLC
                     PLVGGIGILLTGTVVDNWYLWAVKHHVAPMYPHVFPPVVDPALTGAAK"
     gene            complement(1175..1996)
                     /gene="pufL"
                     /locus_tag="VGE92_12105"
     CDS             complement(1175..1996)
                     /gene="pufL"
                     /locus_tag="VGE92_12105"
                     /inference="COORDINATES: protein motif:HMM:NF012352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009521 - photosystem [Evidence IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="photosynthetic reaction center subunit L"
                     /protein_id="HEY1314617.1"
                     /translation="MAMLSFERKYRVRGGTLVGGDLFDFWVGPFYVGFFGVTTAFFAL
                     LGTALIVWGAAIGPTWNIWQINIAPPDLKYGLGLAPLREGGLWQIITICAVGAFVSWA
                     LREVEICRKLGIGYHVPFAFGVAIFAYVSLEVFRPLLLGAWGNGFPYGIFSHLDWVSN
                     TGYQYLHFHYNPAHMLAVSFFFATTLALSLHGSVILSAANPHAGPVQTAEHENTYFRD
                     TIGYSIGSLGIHRLGLFLAISAAFWSAICILISGPFWTRGWPEWWGWWLNLSIWN"
     gene            complement(2090..2281)
                     /gene="pufA"
                     /locus_tag="VGE92_12110"
     CDS             complement(2090..2281)
                     /gene="pufA"
                     /locus_tag="VGE92_12110"
                     /inference="COORDINATES: protein motif:HMM:NF012765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="light-harvesting antenna LH1, alpha subunit"
                     /protein_id="HEY1314618.1"
                     /translation="MWRIWLLFDPRRALIALFTFLFVLALLIHFILLSTDRFNWLGGH
                     AAPSAAAQHMSTLPDTAKP"
     gene            complement(2316..2465)
                     /gene="pufB"
                     /locus_tag="VGE92_12115"
     CDS             complement(2316..2465)
                     /gene="pufB"
                     /locus_tag="VGE92_12115"
                     /inference="COORDINATES: protein motif:HMM:NF040862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030096 - plasma membrane-derived
                     thylakoid photosystem II [Evidence IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="light-harvesting antenna LH1, beta subunit"
                     /protein_id="HEY1314619.1"
                     /translation="MADQSRGSLSGLTESEAKEFHGIFVTSFIIFTVIAIVAHFLVWQ
                     WRPWL"
     gene            complement(2624..4048)
                     /gene="bchZ"
                     /locus_tag="VGE92_12120"
     CDS             complement(2624..4048)
                     /gene="bchZ"
                     /locus_tag="VGE92_12120"
                     /EC_number="1.3.7.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorophyllide a reductase subunit Z"
                     /protein_id="HEY1314620.1"
                     /translation="MLVLDHDRAGGYWGAVYVFTAIKGLQVIIDGPVGCENLPVTSVL
                     HYTDALPPHELPIVVTGLGEEELGQHGTEGAMKRAHRTLDPHLPAVVVTGSIAEMIGG
                     GVTPEGTNIQRFLPRTIDEDQWQSANRAMSWLWTEYGRKKIPQRKPRPENEKPRVNII
                     GPMYGTFNMPSDLAEIRRLVEGIGAEVNLTFPLGSHLQDMPRLIDADANICMYREFGR
                     LLCETLQRPYLQAPIGLHSTTKFLRRLGELLGLDPEPFIEREKHTTIKPLWDLWRSVT
                     QDFFGTASFAIVANETYARGVRHFLETEMGLPCAFSFARRAGVKPDNEAVRKALRESP
                     PLIMFGSFNERMYMAESAVRAIYIAASFPGAIIRRHTGTPFMGYSGATYLVQEVCNAL
                     FDALFNILPLGSELDKVDATPSRLHRELRWSEDAKQALDRMVSSEPVLLRISAAKRLR
                     DAAERAAHGAAAEHVDIHHLTEPA"
     gene            complement(4048..>5362)
                     /gene="bchY"
                     /locus_tag="VGE92_12125"
     CDS             complement(4048..>5362)
                     /gene="bchY"
                     /locus_tag="VGE92_12125"
                     /EC_number="1.3.7.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chlorophyllide a reductase subunit Y"
                     /protein_id="HEY1314621.1"
                     /translation="RMRRTATVLSGSACCVYGLTFTSHFYGARRSVGYVPFNSETLVT
                     GKLFEDIREAVFKLADPALYDAVVIINLCVPTASGVPLQLLPKAINGVRIIGIDVPGF
                     GVPTHAEAKDVLAGAMLRYARTEAVQGPVQAPRGGRNAKPTVTLVGEVFPADPVGVGM
                     MLEPLGLAAGPVVPTREWRELYAALDCAVAAAIHPFYTASLREFSAAGRSIVGSAPVG
                     YDGTAAWLAAVGESCGIASDKIAVAQNRILPAIKAALAKSPIRGRITLSGYEGSELLV
                     ARLLIESGADVPYVGTACPRTPWSDSDREWLQSKGVRLQYRASLEHDLAALHEFRPDL
                     AVGTTPVVQKAKEMAIPALYFTNLISARPLMGAAGAGSLAQVVNAALGNRARFDEMQA
                     FFAGVGGGDTAGIWQQPPQHHPEFRERYKAMLAKLAAKRKSEEMV"
BASE COUNT         1125 a         1739 c         1568 g          930 t
ORIGIN      
        1 gcattcttgc ccgcccgcac cgcgtcgatc cgcggattga tgatttgctc catgcccaca
       61 ccgcgatagc cgccctgcag ggtttcgacg ggcggatgct cgcagccgca caagccgagt
      121 gcagccatca aaaatccgca ggccatcgca cggcgattga tgagagtgcc gagttgcttc
      181 attttgccgc tcccgtcaat gccggatcga ccaccggcgg gaatacgtgc gggtacatgg
      241 gtgccacgtg atgcttgact gcccataggt accaattgtc gaccacggtg ccggtgagca
      301 gaatgccaat cccgccgacc aaggggcaca ggaccgcgaa ccaccaggcc cagcgatgaa
      361 tcgactccat ggttgcgttg aaccccatcg tccaacgcca aaacagcgcg gcgcgctcgg
      421 ccgcggtacc tcgatccacg atctgctcga gctcccgatc tccgcccagg cggctcaccg
      481 ccaaaatggt tccaccgtgc attgcgaaca gcagtgtcga gccgtagaga aacgcgatcg
      541 acaggcagtg aaacgggttg tagaacagat tgccgtagcg aatcgaaaat gccgcggtcc
      601 aatccaggtg cggaaagatg ccgaacggca ccgcttcgcc gaaactgccc atcaggaccg
      661 ggcgaatgat tcccaaaacc agcatcaacc agatcgcgga cagaaacgcc caggagacgt
      721 gagttcccat tcccaaggcg cgcgcccggc gatacatccg cagccaccag agcagcactg
      781 agctggtcaa gaagaagcct gccatgagcc accaaccgcc atcctgcaag cgcgggaaat
      841 gcaggccgtt cgccggcgac ggcggctcga gcgccagcca cggcaattgc ctgatgaatt
      901 gcaccgggtt ccagtggacc gatgcaaaca tgttgagacc gatgatctcg aaggcgatga
      961 aaccgcagat cagcgacagc agcccggtcg tgccgagata aatggggcca atctgcgcgt
     1021 ctccgaacct tccgagcaaa tggctgaagc cacctttgcc ttggcgtccc caagccccgc
     1081 gcggcagcgc gacgccgaca taggcaggcg cgcgaatttg cactcgcgta aatatgtttt
     1141 gatattcagc catgatagtc cccagccagt tgcgtcaatt ccaaatcgac aggttcaacc
     1201 accagcccca ccattcgggc cagccccggg tccagaacgg accgctgatc agaatgcaga
     1261 tcgcgctcca aaacgccgcg ctgatcgcga gaaacaaccc caggcggtgg attcccagcg
     1321 agccgatcga gtagccgatc gtgtcgcgaa agtaagtatt ttcgtgttcc gcggtttgca
     1381 ccggaccggc gtgcggattt gcggccgaca aaatcaccga tccgtgcagc gacagtgcca
     1441 gcgtcgtcgc aaagaagaag ctgacggcca gcatgtgcgc cggattgtag tgaaaatgca
     1501 gatattgata gccggtgttg gatacccagt ccaagtgact gaagatcccg taagggaagc
     1561 cgttgcccca tgcgccgagc agcagcggcc tgaatacttc caaggaaaca taggcgaaaa
     1621 tcgccacgcc gaaagcgaac ggcacgtgat agccgatacc caattttcga cagatctcca
     1681 cttcgcgcaa cgcccacgag acgaaggcgc cgacggcgca gatcgtgatg atctgccaca
     1741 gaccaccctc gcgcagcggc gcgagtccga ggccgtactt caaatccggc ggcgcgatat
     1801 tgatctgcca gatgttccag gttggaccga tcgccgcccc ccatacgatc agcgcggttc
     1861 cgagcagcgc gaagaacgcc gtcgttacgc cgaagaatcc gacgtaaaac ggacccaccc
     1921 agaaatcgaa tagatcgccg ccgaccagcg ttccgccgcg cacacggtat tttcgttcaa
     1981 aactgagcat cgccatgtta gtgctctctt gttgtggtcg ctttcggcag cggtcggagt
     2041 tcggacccgc ttgggcgcgt cccccggccg ccgccgtcgg caaaccgccc tagggcttgg
     2101 ccgtgtctgg cagcgtcgac atatgctgcg cagccgccga gggcgccgcg tgcccaccca
     2161 gccaattgaa cctgtccgtg ctcagcaaaa tgaaatgaat caacaatgcc agcacaaaca
     2221 ggaatgtgaa cagagcgatc aacgctctgc gcggatcgaa aagaagccaa attcgccaca
     2281 ttttctagtc tcctatgcgt atcagcgttc gatgcctaga gccacggccg ccattgccac
     2341 actaaaaagt gcgcgacaat cgcaatcacc gtgaaaataa tgaagctcgt cacgaatatt
     2401 ccgtgaaact ccttggcttc actttccgtc agcccggaaa gcgaacctcg actctggtct
     2461 gccatacctc tcacctccac aagtcaggcc ccatggccgt ccaccgcgct aaagctcgcg
     2521 cgacttaaaa aagccgcggc ggaagaatcc cgccccggca cgcctacgtc cgaaagatct
     2581 tcgggcgaat tctcagcaat gcactgcact ctcgcggccg ccatcaagcg ggctccgtca
     2641 ggtgatgaat atccacatgc tcggccgccg caccgtgtgc ggcgcgctcc gccgcatcgc
     2701 gcaggcgttt ggcagcggag atgcgcagca gcaccggttc ggacgacacc atccggtcaa
     2761 gcgcttgttt cgcgtcctcg ctccagcgca gttcacgatg caaacgcgac ggcgttgcgt
     2821 ccactttgtc caactcagag cccaacggca ggatgttgaa cagcgcatcg aacagcgcgt
     2881 tgcagacttc ttgaaccagg taggtcgcgc cgctgtagcc catgaacggc gtccccgtgt
     2941 ggcgccggat gattgcgccc ggaaaggacg ccgcaatgta gatggcgcgc acagcgctct
     3001 cggccatgta catgcgttcg ttgaagctgc cgaacatgat gagcggcggc gattccctga
     3061 gcgccttgcg cacggcctcg ttgtcgggtt tgactccggc gcggcgcgcg aaggaaaacg
     3121 cgcacggcag gcccatttca gtctcgagga agtgccgcac gccgcgcgcg taggtttcat
     3181 tggctacaat cgcaaaactg gcggtgccga aaaaatcctg agtgacggag cgccacaagt
     3241 cccacagcgg cttgatggtg gtgtgtttct cgcgctcgat gaaaggttcc ggatcaagcc
     3301 cgagaagctc acccaacctg cgcaagaatt tcgtcgtgct atgcaggccg atcggcgctt
     3361 gcaggtaagg acgctgcaat gtttcgcaca acaggcgccc aaactcgcgg tacatgcaga
     3421 tattcgcatc ggcgtcgatc aaacgcggca tatcctgtag atggctgccg agcgggaacg
     3481 tcagattgac ctcggcgccg atgccttcga ccagacggcg gatctccgca aggtcgctcg
     3541 gcatgttgaa cgtgccgtac atcggtccga tgatgttgac gcgcggtttc tcgttctccg
     3601 ggcgcggctt gcgttgcgga atttttttcc tgccgtactc ggtccacagc caggacatcg
     3661 cgcgattggc gctttgccat tggtcttcat cgatggtgcg cggcaggaac cgctgaatgt
     3721 tggtgccttc gggagttacg ccgccaccga tcatctctgc gatcgaaccg gtcaccacca
     3781 ccgcaggcag gtgtgggtcg agcgttcgat gcgcgcgctt catggctcct tcggtgccgt
     3841 gctggcccaa ttcttcctcg cccaagccgg tgacgacgat cggcaactcg tgcggcggca
     3901 gcgcgtcggt gtaatgcagc accgaagtga ccggcagatt ctcgcaaccc accggcccgt
     3961 cgatgatcac ctgcaacccc ttgatcgcgg tgaacacgta caccgcgccc caatagccgc
     4021 cggcccgatc gtggtcgagc accagcatca gaccatctcc tcggatttgc gttttgccgc
     4081 gagcttcgcg agcatcgcct tgtagcgctc gcgaaactcc ggatgatgct gcgggggttg
     4141 ttgccaaatc ccggcagtgt cgccgccgcc gacgcccgcg aagaaagcct gcatttcatc
     4201 gaagcgcgct cgattgccga gtgccgcgtt gactacctgc gccaacgaac ctgcacccgc
     4261 ggcacccatc agcggccgcg ccgaaatcaa gttggtgaag tacagcgcgg gtatcgccat
     4321 ctccttcgct ttttgcacca cgggcgtggt gcctaccgcg agatccggcc tgaactcatg
     4381 cagcgctgcc agatcgtgtt cgagcgaggc gcggtactgc agccgcacac ccttggactg
     4441 cagccactca cgatcggaat ccgaccaagg tgttcgcggg catgccgttc cgacataagg
     4501 cacgtcggcc ccgctctcga tcaagaggcg tgccaccaac agctcggaac cctcgtagcc
     4561 cgacaaggta atccggccgc ggatgggcga cttcgcgagc gccgccttga tggccggcaa
     4621 tatacgattc tgcgcaacgg caatcttgtc cgaggcgatc ccgcaagact cgccgaccgc
     4681 ggcgagccag gcggcggtgc cgtcgtatcc caccggcgcc gatccgacga tgctgcgccc
     4741 ggcggccgag aactcgcgca gggaagccgt gtaaaaggga tggattgccg cggccaccgc
     4801 gcaatcgagc gcggcgtaga gctcacgcca ttcgcgggtc ggcaccaccg ggcccgccgc
     4861 aaggcccagc ggttcgagca tcatgccgac accgaccgga tccgccggaa acacttctcc
     4921 caccaaggtc actgtgggct tggcattgcg gccgccgcgc ggcgcctgca ccggcccttg
     4981 aaccgcttcc gtgcgggcgt atctcagcat ggctccggcg agcacatcct tggcctcggc
     5041 atgggtcggc acgccaaatc cgggcacgtc gatgccgatg atgcgcacgc cgttgatcgc
     5101 cttcggcagc agttgcagcg gcactccgga agcggtcggt acgcatagat tgatgatgac
     5161 caccgcgtcg tacagcgccg ggtcggcgag cttgaatacc gcttcgcgaa tgtcctcgaa
     5221 gagcttgcct gtcaccaatg tttccgagtt gaaaggtacg tagccgacgc tgcggcgcgc
     5281 gccgtagaaa tgcgaggtga aggtcaagcc gtagacgcag cacgcggaac ccgacaacac
     5341 cgtcgcggtg cggcgcatac gc
//
LOCUS       DASWZT010000424        13667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_75753, whole genome shotgun sequence.
ACCESSION   DASWZT010000424 DASWZT010000000
VERSION     DASWZT010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13667
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75753"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..2187
                     /locus_tag="VGE92_12130"
     CDS             31..2187
                     /locus_tag="VGE92_12130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1314622.1"
                     /translation="MRNQPLHHALLIRLAAPQVLYPVIAVILLLVIWSTAFGVIKVKE
                     ADAAHAAAASTSVLLSAYEAQAVRALTQIDLTLNLVKYWPQHTAGHMLAELKAKGLLP
                     PDLIFIVSIADVDGNIVDTTDSSAEQNIAAQDIFLKQRDGDVFFIGQLPRGPTRDAKL
                     QFSRRLNAANAAFDGAVIVSVEAAYFVSGYDPSKLGEHGVLSMIGADDISKVRRTGDG
                     MFSGESIDYAAAIQDPDAIATDATVSISSWDQVQRWTSARELYGFPLAVLVGLSVDEQ
                     MAPAHRQTRVYMEWAALASALVIVLTTLLGRVSWQLAQSREMFKLMAESTKAIPFTLD
                     LTRSCFSYIGAVGIADSGIPERQWKVLGALDKVIPRNTNHETRQRFDECNSGPFEFVT
                     PLSMGNNHRTEVRWTGTCVRVAKAKVLRGLMLDVTELRRLGRELAAAQKLESVGRLAA
                     GVAHEINTPVQFVSDNVEFVRASLTDLAAVIHAYRDLKLAVQSAGDVTAAARLAGNAE
                     QAADLDYILENAPQALDSSIDGLGRIATIVRSMKEFAHPDQAHKTLTDLNQAIRSTLI
                     IAHNEYKYVAELDAQYGELPPIPCYLGEINQVILNLLVNAAHAISDVVKNTGSLGKLT
                     VRTRLDGNEVEISISDTGTGIPESARDKIFDPFFTTKEIGKGTGQGLAIAHSVIVKKH
                     GGTLRFETECGKGTTFFIRLPIDAVIDASAATELAA"
     gene            2598..2786
                     /locus_tag="VGE92_12135"
     CDS             2598..2786
                     /locus_tag="VGE92_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314623.1"
                     /translation="MPKIAVYRFSCWDRDTGETVEYPRFATLKTIKGRDGRNIEASRR
                     IVEDSDIDDQGFLKNIQP"
     gene            2869..4497
                     /locus_tag="VGE92_12140"
     CDS             2869..4497
                     /locus_tag="VGE92_12140"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1314624.1"
                     /translation="MSAPQAKDPPISSPAPAVATAWAPFRIRTFAVIWTATVVSNIGG
                     WMYNVASGWLMTSLDPDPFIVSMVQVANSLPMFLFAIPAGALADIVNRRRLLIFGESA
                     IMITSTAFAALVWLHLITPVSLLVFSFVVTVGSAITAPGWQAVVPDLVPKADLPAALA
                     ANSVGINVSRAVGPAIGGLLVGAIGIAAPFWFNGFSNLGVIVALVRWRLPKKSGTPLP
                     PEGFGNAMRTGLRHARYNPNLSATLIRATAFFVFASAYWALLPLVARNRIAGGPALYG
                     ILLGAIGAAAVGGAFLLRWLRGKLGADRLLSIATLASAVATGLFALARDSATAVAASL
                     IAGASWIAAVSSLNVSAQIALPEWVRGRGLAVYVTVMFGALTLGSAIWGELAAVTSLS
                     GALFIAAVGAILAIPLTGAWKLQAGAADDLSPSMHWPAPITSGAVEPDRGPVLVTVEY
                     HIDPKNREPFLHALGRSARERRRDGAYDWGIFEDPAKEGRFIETFLMDSWLDHLRQHQ
                     RVTKADRIAEQVVRRFQVGDGPKTTHLIGAHARR"
     gene            4600..4962
                     /locus_tag="VGE92_12145"
     CDS             4600..4962
                     /locus_tag="VGE92_12145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1314625.1"
                     /translation="MQQIVIYEEDYLTRTLLREWLSEAGYRVRGGTPFEPTCDSRADL
                     VIASVYMPKNAGVQAIRDIQAAHPGTPLIAISGQFRSGLCAGGATAQSLGVQQVIAKP
                     LIHRDLLEAVRGIMGVPE"
     gene            4978..7506
                     /locus_tag="VGE92_12150"
     CDS             4978..7506
                     /locus_tag="VGE92_12150"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1314626.1"
                     /translation="MPSPSGQVPPDTGSIRFKRLRLRVVVFGVLVIMAFAGSSTYDAW
                     SSYQHTVAAIDREIGNISNTLAEQTAWTLQAVDLLLLDTARWYRSDSKAIPSERLEAA
                     LAIRTAGVQPVRLVTIVDSQGNQRYRSREFSAPNNNVSDRSYFIAQRDGTAKGLFMSE
                     PLVTRSEGRPGVIFSRRIDDENGQFAGVVTAIVDLEDFNQFYRSVDVGRGGAIQLLRD
                     DGTLLVRHPATPAAVGKQYLELAADTVAPAGRLLNPIDGRTDFVAVSPVRNTRLRLAV
                     TRESALALQTWRDETIRVAVRTLIIVLLGVLTVMALLRQLRRVAAGERALRESEERYA
                     LAMEGANEGHWDWDVRGDRLFLSPKMKLLGGQRADSEIGSRAEWLAKIVMHPDDSPRF
                     EAAFRDHFEGRTPRFECEYRVRQPNGDWHWLFARGRCLVDSAGAPVRFVGSAIDVTAQ
                     KQAQLDNEQLEIQLRQSQKMEAIGTLAGGIAHDFNNILGAILGYGELALEHCAPTSEL
                     RRYLDNVMHATERAKRLVERILGFSRSGLGDRLPVNVESVVREALELLEASLPADIRL
                     QRRIDAGNAAVIGDPTYLHQVVMNLCTNAIQAMERGGVLSVALERHEQYEHRTLSRGS
                     VVPGPYVRLIVSDTGHGIPPMVLERMFDPFFTTKDVGQGTGLGLSLVHGIVTDLGGAI
                     DVTTTAGVGTQIEIWLPTAGQAPAPSVETSQALPRGKGETVMIVDDERPLVALAEEII
                     AELGYEPVGFDSSSAALDAFRATPERFDVVLTDESMPDLIGTELAQATRRFRPTIPII
                     LMTGYGGPQITNRAADIGVNEVLRKPLRRRDLAESLARALESVV"
     gene            7526..8257
                     /locus_tag="VGE92_12155"
     CDS             7526..8257
                     /locus_tag="VGE92_12155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1314627.1"
                     /translation="MSQTISPNILVIDDDPQIRSLLKEYLAENGLRVSVASSGEEMSK
                     VLGDEAVDLVVLDLRLAGEDGMAIARSLRDQSAIPIVMLTGVRDEADRVMGLELGADD
                     YVTKPFSPRELLARIRTVLRRTKSAVPGSARQREIRAYRFGAFELNLRTRRLKQRDGG
                     QIVLTNGEFNLLAALLAAPERILTRDQLLEASRVYDNEVYDRAIDIQVLRLRRKIESD
                     PSQPQFIVTERGAGYIFRAPVDVVY"
     gene            8538..9692
                     /locus_tag="VGE92_12160"
     CDS             8538..9692
                     /locus_tag="VGE92_12160"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEY1314628.1"
                     /translation="MNHSLLSHGDAPTMTPMYRVTAAARLRVSMEWLRRRYFLLVGAL
                     TVALAVAGVAHYWLSARFVVSTNDAYVQADSTIVASRVSGYVSAVMIQDDQSVKAGQI
                     LALIDDRDFSAALDQARADARAAQAAVDDLQAQLIEQATLIDRARAGVAESQAALELA
                     SLNQVRYRKMAGIGFGSEQQSQQADAEAQERSANLARDRAALASAQAQVRVLQAKLAQ
                     AQALRDRSQAAEHRAALELGYTTITAPIDGIVGARSVRLGQYVQAGTQLTALVPLREV
                     YIMANFKETQLTRVRAGEPVRVRVDTFPGIDLNACVDSLAPASGLEFSLLPPDNATGN
                     FTKIVQRIPVKIRFDSSEPLAGRLRPGMSVEASVDTNSAARRNPCRAPRL"
     gene            9671..11311
                     /locus_tag="VGE92_12165"
     CDS             9671..11311
                     /locus_tag="VGE92_12165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEY1314629.1"
                     /translation="MSSTAALDRPTSVAPGASAPAVAAGSPTPALEEVSFKTWVAVIG
                     STIGAFLAILNIQVVGASLADIQGGIGAGIDDGGWITTSYLIAEIIVIPLSGWLASVF
                     SLRTYLLTSTALFLALTAACAFAQTLGQMIVIRAAQGFAGGVLIPLAFTIIMTKLPRS
                     KHPIGLAIYSIAVVFAPSIGPALGGYFSDNFGWQSIFFLSLIPGALMIAMLWYALDPA
                     PRQFHLLAEGDWLGVITMALGLGSLETVLEEGNKDDWFGSPFIVRLSVVAVLSLAAFI
                     YLELKRPTPLLNLRLLKRRNFGLGTCANFVFGFSMFGWIYLIPQYLSRLQGYSSQEIG
                     GVMVWLGLPQLFLIPFIPKVMQRVQVRGLVIAGYSLFIGGSLLAMFLSDDFSGPEFLG
                     SSLIRALAQAMTMAPLSAMAIAGIEPRDAGSASALFNATRNLGGAVGIALLQTLLTKR
                     EQFHSDVLSSQVTLLATATRQRLDHLADYFLAHGASDRNLANSEAAVAIGRVIRHQAF
                     MLGFSDVIIIQSAVLGLGLAAVLFLRGTARSSANGESH"
     gene            11308..12795
                     /locus_tag="VGE92_12170"
     CDS             11308..12795
                     /locus_tag="VGE92_12170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY1314630.1"
                     /translation="MSDPRTVVAPVTAALALLLAAGTLSGCATSPRYGRPADPVQTQY
                     LDPANLPPVQTAAPVPHVSMGNGSAERWWTLLGSPDIDRLVTLALQNNRSLATANAHL
                     AAAREHLRAARGAWYPQVDAGAGAQHTRFGATSLGPLAKDFPPFSAYAAGVEVSYDFD
                     FFGSTRSRVEQAAASAQYESAQLEAAALSVSGNVVLEALQVAALRAQIRVAEQIVADD
                     EHMLGLIRAAREAGAVSQIDVLSSQSQTDHDRTLLPPLRQQLSAAQDVLAVLLGAAPA
                     DGTPREVELGTLRPADDLPVALPSELVRRRPDIDAAEAQLHEAGAAVGIATANLYPNF
                     TLSARLAGEGLLNGGPSETAWNLLGGLTAPIFHGGALSAERRAAQDDYEAAFAAYQQT
                     VLNAFGQVAAALQALVNDAELLSAQQRALDSAGASLTLTRQAYKAGNAGYTQVLDAER
                     LHSQAELGRVQADGQRYIDTAKLLLAAGGRSGVAPNDGSRLTFHP"
     gene            13036..13539
                     /locus_tag="VGE92_12175"
     CDS             13036..13539
                     /locus_tag="VGE92_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314631.1"
                     /translation="MAACGAGGTGIGLYKGALFAEINDKIVRYSLRAGSIVPQGPAVP
                     VVTGLPLSGDHPMHPFTIDGDGFLFIDVATPTNACQRKNRTLKSPGIVPCPELETRGG
                     IWRYDANKANQAFSPAERFATGIRNAEGFAIDAAGRMFVTQHGRDQLHANWPDLYQPR
                     GGGYAAR"
BASE COUNT         2515 a         4195 c         4256 g         2701 t
ORIGIN      
        1 ggtcggcgct cttgccatat ggaattgaga atgcgtaatc agcctttgca ccacgccttg
       61 ttgatcaggc tggctgcacc gcaggttttg tatccagtga tcgccgttat tttgctgctc
      121 gtgatctgga gcaccgcctt cggcgtcatc aaagtcaagg aagctgacgc cgcacatgcc
      181 gcggcggcat ccacgagcgt gctattgagc gcctacgagg ctcaggccgt ccgggcgttg
      241 actcagatcg atctgaccct gaatctggtc aagtactggc cgcagcacac agcaggccac
      301 atgctcgcgg aactcaaggc taagggattg ttgccgccgg atctgatctt catcgtcagc
      361 attgctgacg tcgatggcaa catagtggat accacggatt cttccgcaga acagaatatc
      421 gccgctcagg acatctttct caagcaacgc gacggcgatg tttttttcat cggacagcta
      481 ccacgggggc ccacccgcga cgcaaaattg cagttcagcc gacgactcaa tgccgcgaat
      541 gcagcgtttg acggcgcagt gatcgtatcg gttgaagcgg cttacttcgt cagtggttac
      601 gatccctcca agctcggcga gcacggagtg ctcagtatga tcggcgccga cgatatctcc
      661 aaagttaggc gcaccggaga tggcatgttc tcgggcgaat cgatcgacta tgctgccgcg
      721 atccaggatc ctgatgcaat cgcgaccgac gctacggtct cgatcagcag ctgggatcaa
      781 gtgcaacgct ggacaagtgc cagagaactc tatggctttc ccctcgcggt actagtggga
      841 ctgtcggtgg acgaacaaat ggcccccgcg catcggcaaa cgcgcgtcta tatggagtgg
      901 gcggctctgg caagcgccct ggtcatagta ctaacgaccc ttttgggccg cgtcagctgg
      961 caacttgccc aaagtcgcga gatgttcaaa ctcatggcgg aaagcaccaa agcgattccg
     1021 ttcaccttgg atctgacgcg cagctgtttc agttatatcg gcgccgtagg cattgccgac
     1081 tcaggaattc cagaacggca gtggaaggtg ctgggtgctt tggataaggt cattccccgc
     1141 aacacgaatc acgagacgcg gcaacgattc gatgagtgca acagcgggcc gtttgaattt
     1201 gtgacccctc tgtccatggg caacaatcat cgcactgagg tgcgctggac gggaacttgc
     1261 gtgcgggtgg cgaaggccaa ggtgttgcgc ggtctcatgc tcgatgtcac cgaactgcgg
     1321 cgcctcggcc gcgaactcgc cgcggcccaa aagcttgaat ccgtcggccg gcttgctgca
     1381 ggcgtcgccc atgagatcaa caccccggtt cagtttgtct ccgataacgt ggagttcgtg
     1441 cgtgcttcat tgactgatct ggccgccgtg atccacgcct accgcgacct caagctcgcg
     1501 gtgcaatcgg caggtgatgt gaccgctgcg gcgcgccttg ccgggaacgc cgaacaggct
     1561 gcagacctcg actacattct ggagaacgca cctcaagcgc tcgacagctc gatcgatggc
     1621 ctcgggcgca ttgcaacgat cgtgcgctca atgaaggaat tcgcgcatcc ggaccaagcc
     1681 cataagacat tgaccgattt gaatcaagcc attcgcagca ccctgattat tgcccacaac
     1741 gagtacaagt acgtcgccga actcgacgcg cagtacggcg agttgccgcc aattccgtgc
     1801 tatttgggtg agatcaacca agtgatcctc aatttgttgg tgaacgcagc gcatgcgatt
     1861 tcggatgtcg tcaagaacac gggcagtctg ggcaagctca cggtgcggac gcggctcgac
     1921 ggtaacgagg ttgagatttc gatttcagat accggcaccg gcatccccga atcggcgcgc
     1981 gataaaatct tcgacccatt ctttacgacc aaggaaatcg gcaaggggac cggacaaggg
     2041 ctggccatcg cacacagcgt catcgtcaag aaacacggcg gtacgctgcg cttcgagacc
     2101 gaatgcggca aaggcactac tttctttatt cgactcccca tcgacgcggt gatcgatgcg
     2161 agcgctgcaa ctgaactcgc ggcgtgattc tgagacgccc accggcaaag tgcgctagca
     2221 gacagacagc gcgccgcagc atctggcagt gtttgtgaca tgcaggatct tctgcaagac
     2281 atcgacttcg actattatcg ttcggcacgg ttactcattg tgcgcgcgga gatagcgcag
     2341 ttgcgtcagc aatcagcggc gttggcgcgc actgaacggc agtgcaaaac tccccccatt
     2401 ggaaaccggg atcaaccaga atccgacggt cgtcacgttc ccggaagctg accgccgcac
     2461 attttgcagg cactacgcga gcaatccgtc cttggactct tgcggcgccg caatttgcat
     2521 atccacctcg atggccatgg tccatggtct tcacccaccg gcagcgctac tccgacgtcg
     2581 agtgcacaag gagtcctatg cctaagatcg ccgtttatcg tttctcctgc tgggacagag
     2641 ataccggcga gactgttgag tatccgcgct ttgccacttt gaagacgatc aaggggcgcg
     2701 atggcagaaa cattgaggct tcgcggcgaa tcgtcgaaga cagcgacata gacgaccagg
     2761 ggtttctcaa gaacatccaa ccctagtaaa cgctcaatcg atcggatacg gcccccggga
     2821 agccttcctg cgggttacca tgagtgcgct taagattgga cgagcaggat gagcgcccct
     2881 caagcgaagg atcctccgat tagctcccct gcgcccgcag ttgcaaccgc gtgggctccg
     2941 tttcgcatcc ggacattcgc ggtgatctgg accgcgaccg tggtctcgaa catcggcggc
     3001 tggatgtaca acgtcgcatc aggctggctc atgacgagcc tcgacccgga ccctttcatc
     3061 gtctccatgg tgcaggttgc caacagcctg cccatgtttc tgttcgcgat tcccgcgggt
     3121 gcgctcgcgg acatcgtgaa ccggcgccgc cttctgatct tcggcgagtc tgcgatcatg
     3181 atcacctcga ccgccttcgc cgcgctcgtt tggcttcacc tcatcacgcc ggtcagtttg
     3241 ttggtattca gtttcgtcgt gacggtgggc tccgccatta cggccccggg gtggcaggcg
     3301 gtggtgccgg acctcgtacc gaaagctgat ctgccggccg cccttgcggc aaacagcgtg
     3361 ggcatcaacg tcagccgtgc cgtcggacct gccatcggcg gtctgctggt cggggcaatc
     3421 ggaattgcgg cgccattctg gttcaatgga ttcagtaact taggcgtcat cgtagccctt
     3481 gtgcggtggc gcttgccgaa gaaaagcggt acgccgctgc cgcccgaggg tttcggcaat
     3541 gccatgcgca ccggtcttcg gcacgcgcgc tacaacccca acctcagcgc gactctgatc
     3601 cgggcgactg cattcttcgt ctttgccagc gcttactggg cgcttttgcc gctggttgca
     3661 cgcaaccgga tcgccggggg tcctgcgctg tacgggatat tgctcggcgc catcggagcc
     3721 gcggctgtgg gcggcgcatt cctgctgcgc tggctcaggg gcaagttggg cgcggatcga
     3781 cttctctcga tcgccacgct ggcaagtgcg gtggcgaccg ggctgtttgc gctcgcccgg
     3841 gattccgcaa ccgcggttgc ggcgagtctc atcgcgggcg cgtcgtggat cgcggcggtg
     3901 tcgagcctca atgtgtccgc acaaattgcg ctgccagaat gggtccgcgg ccgaggcctc
     3961 gcggtatacg tcactgtgat gttcggtgca ctgacgctgg gcagcgcgat ctggggcgag
     4021 cttgcagccg tgaccagctt gagcggcgct cttttcatcg cggcggtggg tgcgatactc
     4081 gcgattccgc tcactggggc ctggaagcta caggcgggcg cggcggacga tctgtcgccc
     4141 tcgatgcact ggccggcgcc gatcacgagc ggtgcggtag agccggaccg tgggcccgta
     4201 ctggtgacag tcgaatacca catcgatccc aagaaccgcg aacccttttt gcatgcgctc
     4261 gggcgcagcg cgcgcgagcg ccgtcgggac ggcgcctatg actggggaat attcgaggac
     4321 cctgcgaagg agggccgctt catagagacc tttctcatgg actcctggct cgatcaccta
     4381 cgccagcacc agcgtgtgac caaggccgat cgcatcgcgg aacaagtggt tcgacgcttt
     4441 caggtcggcg acgggcctaa gacaactcat ctgatcggcg cgcatgcgcg acggtaagtc
     4501 cattgccgcc aacgaatcgg cggtgcattt caactgaaac aatgtcaaag ctacctgaaa
     4561 ttgcagcgtg cggagcctgc cgaaagatag cttcgaccta tgcagcagat tgtgatctac
     4621 gaggaagact acctcactcg caccttactc cgcgagtggc tgagcgaggc aggctatcgc
     4681 gtccgcgggg gtacgccatt cgagccgact tgcgattccc gcgcggatct cgtgattgcg
     4741 agcgtctaca tgcccaaaaa tgccggggtg caagcgatac gcgatattca ggcagcgcac
     4801 cccggcacac ccctgattgc gatctccggc cagttccgat ccggactttg cgcgggagga
     4861 gcgacggctc aaagtctcgg ggtacagcag gtgatcgcaa agcctctgat ccaccgtgat
     4921 ctgctggaag ccgtgcgagg tatcatgggc gtgccagagt agacgcggcg cagcgacatg
     4981 ccctcaccca gcggccaagt tccaccggat accggctcga tccgattcaa gcgcctgcgg
     5041 ctccgcgtgg tcgtgttcgg cgttctcgtc atcatggctt tcgccggctc atccacctat
     5101 gacgcctgga gttcgtacca gcacacggtt gcagccatcg atcgcgagat cggcaacatc
     5161 tcgaataccc ttgcggaaca aaccgcgtgg accttgcagg cggtcgatct gttgttgctc
     5221 gacaccgcgc gatggtatcg cagcgacagc aaggcaattc catccgagcg cctggaggca
     5281 gctcttgcga tccgcaccgc cggcgtgcag ccggtgcgcc tcgtgacgat cgtcgattcg
     5341 cagggcaatc aacgttatcg ttcacgtgaa ttctccgcgc ctaataacaa tgtatccgac
     5401 cgctcgtact tcatcgcaca gcgggacggc actgccaagg ggctcttcat gagcgagccc
     5461 ctggtgactc gttccgaagg ccgccccggt gtgatctttt cgcgccggat tgatgatgag
     5521 aacggccagt tcgcgggcgt cgtcacggca atcgtcgatc ttgaggattt caatcagttc
     5581 tataggtctg tggatgtcgg ccggggcggc gcgattcagc tgctacgcga cgatggtacc
     5641 ttgctggtac gccatccagc gactcctgcc gccgttggca aacaatatct cgagctggcg
     5701 gcggacaccg tcgcaccggc cggccgcctg ttaaatccga tcgacgggcg cacggatttc
     5761 gttgcggtct cccccgtgag gaatacccgc ttgagactgg cagtaacccg ggaatccgct
     5821 cttgcgttgc agacatggcg cgacgagacg atccgcgttg ctgtgcgcac gctcattatc
     5881 gtattgctcg gcgtgttgac cgtcatggcg ctattgcgcc aactgcggcg ggttgccgcc
     5941 ggcgaacggg cgttgcgtga aagcgaggag cgttatgcgc tcgccatgga gggagcgaac
     6001 gagggtcatt gggattggga cgttcggggc gatcgtctct tcctttcgcc gaaaatgaag
     6061 ctgctgggcg ggcaacgtgc agacagcgag atcggctccc gggccgagtg gttggccaaa
     6121 atcgtcatgc atcccgatga ttcgccgcgc ttcgaagcgg cgtttagaga tcacttcgag
     6181 ggacgcacgc cgcgcttcga atgcgagtat cgcgtgcggc agccgaacgg cgattggcat
     6241 tggttgttcg cgcgcggacg ctgtctggtc gactccgcgg gcgcacctgt tagattcgtc
     6301 ggctccgcga tcgacgtcac cgcacaaaag caggcgcagc tcgacaacga acaactggaa
     6361 attcaattga ggcagtcgca aaagatggag gcaatcggca cgctggccgg cgggatcgcc
     6421 cacgacttca ataatatcct gggggccatc ctggggtacg gtgagctcgc gcttgagcat
     6481 tgcgcgccga cgagcgagtt gcggcgctac ctcgacaatg tcatgcacgc gaccgaacgc
     6541 gccaagcgtc tggtcgaacg cattctgggg ttcagtcgca gcggcctcgg tgaccggctt
     6601 cccgtaaacg tcgagtcggt tgtccgcgag gccctcgagc tcctcgaggc atcgctgccc
     6661 gcggacatcc ggctgcagag gcgaatcgat gcaggtaatg cggcagtgat tggagacccc
     6721 acctatttgc atcaagttgt gatgaatcta tgcaccaacg ccatacaggc catggagcgc
     6781 ggcggcgtgc tgagcgttgc tctcgagcgc catgaacagt acgagcaccg gacgctgtcc
     6841 cgcggttcgg ttgtgcccgg accctatgtg cggctcatcg tcagtgacac cggccacgga
     6901 atcccgccca tggtcctcga gcggatgttc gatccattct ttaccaccaa ggatgttggg
     6961 cagggtacgg gtctcggcct gtccctggtg catggcatcg tgaccgacct gggcggcgcg
     7021 atcgacgtca ccaccaccgc gggcgttgga acccaaatcg agatctggct gcccaccgcg
     7081 ggtcaggccc cggccccgtc cgtcgagact tcacaagcgc tgcctcgcgg caagggcgaa
     7141 accgtgatga tcgtggatga tgagcgccca ttggtggcgc tcgccgagga gatcattgcc
     7201 gagcttggat acgagccggt cggattcgac tcgagcagcg cggcgctgga tgcatttcga
     7261 gccacgcccg agcgattcga tgtggtgctt accgacgaat cgatgccgga tctgatcggc
     7321 accgagctcg cccaggcgac ccggcggttt cgccccacga ttccgatcat cttgatgacc
     7381 ggatacggcg gcccgcagat cacgaaccgc gcggccgaca tcggcgtgaa cgaggtgttg
     7441 cgcaagccgc tgcgccgtcg ggatctggcc gaatcgcttg cgcgagccct ggaatccgtt
     7501 gtttgatgta ttggaggtaa ctctcatgtc gcagaccatc agtcccaaca ttctggtcat
     7561 cgacgatgac ccgcagatcc gctcgctctt gaaggagtat ctcgcggaga atgggctgcg
     7621 cgtcagcgtc gcatcgagcg gcgaagagat gtccaaagtt ctcggcgatg aggcggtcga
     7681 tctcgtcgtc ctggatctac gattggccgg cgaggatgga atggccatcg cgcgatcgct
     7741 tcgcgaccaa tcggcgatac caatcgtcat gctcaccggc gtgcgcgacg aagccgaccg
     7801 ggtgatggga ctggaactcg gtgcggatga ctacgtcacc aaaccattca gcccccggga
     7861 gctgctggca cgcattcgca cggtgctgcg gcggaccaag agcgcagttc cggggtcggc
     7921 gcgccagcgc gaaattcgcg cgtaccggtt cggcgccttc gaactcaatc tgcgtacgcg
     7981 gcgcctcaaa cagcgcgacg gcgggcaaat tgtcttgacc aacggcgagt tcaatttgct
     8041 cgccgcgttg ctcgcggcgc cggagcgaat cctcacacgc gatcagctct tggaggcgag
     8101 tcgggtgtat gacaatgaag tctacgaccg ggcgatcgat atccaagtgc tgaggttgcg
     8161 ccgcaaaatc gaatcggatc cttcgcaacc ccagttcatc gtcaccgagc gcggcgcggg
     8221 ctatatcttc agagcgccgg tcgatgtggt gtattgatcc gacgagcgtt tcgactgaaa
     8281 taatcgcggg agcttccgca atcgatcagt aacctgtccc cgccactgtt actcctgaaa
     8341 cgagccatcg gctcgccgaa catgaacagg agtagcgctg tgagtacacc ctctgccgat
     8401 cacgattcct cccgcgacgc cccggcgccc tctggcggag gtggcaacaa cccaatatgg
     8461 ctcatggtcg gtgccggcgc tctgtttttt gcattggcgg cagcgctcct cgcgtccggt
     8521 tagggagcct gcggcgcatg aaccattccc ttctgtcgca tggcgatgca ccgacgatga
     8581 cgcccatgta tcgggtaacc gcggcggcgc gattgcgcgt gagcatggag tggctgcgcc
     8641 ggcgttattt tctgttggtc ggcgcactca cagtggctct cgccgtcgcc ggcgtcgcgc
     8701 actactggtt gtcggcccgc ttcgtggtgt cgaccaatga tgcgtatgtc caagctgatt
     8761 cgaccattgt cgcatcgcgc gtatcgggct atgtgtcggc ggtaatgatc caggatgatc
     8821 agtcggtgaa ggccggtcag atactggcgc tgatcgacga tcgggatttt agcgcggcat
     8881 tggatcaggc gcgagctgac gctcgagccg cgcaggcagc ggtcgatgac ctgcaagcac
     8941 aactcatcga gcaggccacg ctcatcgaca gagcacgcgc gggcgtggct gagagccagg
     9001 cggccctgga gctggcgagc ctcaatcagg tgcgctatcg gaaaatggcc ggcatcggct
     9061 tcggatcgga gcagcaatcc cagcaggccg acgcagaggc gcaggagcgc agcgccaatc
     9121 tcgcccgaga ccgggccgcg ctcgcctccg cgcaggccca ggtgcgagta ctgcaggcga
     9181 agctcgcgca agcccaggct ctgcgcgatc gctcgcaggc tgccgagcat cgcgcggctc
     9241 tggaactcgg ttacaccacc atcacggcgc ccattgacgg cattgtcggc gcacgcagcg
     9301 ttcgtctcgg ccagtacgtc caggccggca ctcagttgac ggcgctggtg cccttgcgcg
     9361 aggtatacat catggctaat ttcaaagaga cgcaactgac gcgggtgcgc gccggcgagc
     9421 cggtgcgggt acgcgtggac accttccccg gcatcgactt gaatgcctgc gtcgatagcc
     9481 tcgcacctgc cagcggactg gaattcagcc tgctgccgcc ggataacgcc accggaaatt
     9541 tcaccaagat cgtacaacgc atcccggtca agatccgctt tgattcgagc gagccgttgg
     9601 ccggtcggct gcggccggga atgtcggttg aggcctccgt cgataccaat tcggccgcga
     9661 ggcgaaaccc atgtcgagca ccgcggctct agatcggccg acgtcggttg cgccgggcgc
     9721 gtcggcgccg gccgttgcgg ccggttcgcc gacacctgct ctcgaggagg tgtcgttcaa
     9781 gacctgggtg gcggtgatcg gctcgaccat aggcgccttt ctcgccatcc tcaacatcca
     9841 ggtggtcggt gcgtcgctgg cggatattca aggcggcatc ggcgccggaa tcgacgacgg
     9901 cggatggatc acgacgtcct atctcattgc cgagatcatc gtgatcccct tgagtggttg
     9961 gctcgcgagc gtgttttcgc tgcgtacgta cttgctgacc agcaccgccc tgttcctcgc
    10021 attgaccgcg gcttgcgcat tcgcgcagac cttaggccag atgattgtca ttcgagccgc
    10081 gcaaggattt gcgggcggtg tgctgattcc gcttgccttt accatcatca tgaccaagct
    10141 gccgcgctcc aagcacccga tcggccttgc gatttattcg atcgccgtgg tctttgctcc
    10201 ttcgatcgga cccgcactcg gcggctactt cagcgacaat ttcggctggc agtcgatctt
    10261 ctttttgagc ctcatccccg gtgcgctgat gatcgccatg ttgtggtatg cgctcgaccc
    10321 ggcgccccgt cagttccatc tgctggccga aggagattgg ctcggcgtaa ttacgatggc
    10381 gctgggcctc ggttctctgg agaccgtact cgaagaggga aacaaagacg actggttcgg
    10441 ttcgccgttc atcgtgcgcc tctcggtggt ggcagtcttg agcctggccg ctttcattta
    10501 cctagagctg aaacgcccca ctccgctgtt gaatctgcgt ttgctgaagc gccgcaactt
    10561 cggactcggc acctgtgcga atttcgtctt cgggttctcg atgttcggtt ggatctatct
    10621 cattccgcaa tatctgtcac gcctgcaggg ctacagctcc caggaaatcg gcggcgtgat
    10681 ggtctggctc ggcctgccgc aattgttcct gatcccgttc atccccaagg tcatgcagcg
    10741 agttcaggtg cgtgggctgg tgatcgccgg atactctctt ttcatcggcg gcagcttgct
    10801 ggcgatgttc ctgtccgatg acttttccgg gcctgaattc ctgggctcga gtttgatacg
    10861 cgccttggcc caggcaatga ccatggcgcc actgtcggca atggccatcg ccggaatcga
    10921 gccgcgagat gcagggtccg catctgcgct gttcaacgcg acgcgcaatc tcggcggcgc
    10981 cgtcggaatc gcgctgctgc agactcttct gaccaagcgc gagcagtttc actccgacgt
    11041 gctctcctcg caagttacgc tgctcgcgac ggccacacgt cagcggcttg accatctcgc
    11101 cgactatttc ctggcgcatg gtgcaagcga ccggaacttg gcgaattcgg aagccgcagt
    11161 cgcgatcggc cgcgtaatcc gccatcaggc gttcatgctg ggattcagcg acgtcatcat
    11221 cattcaaagt gcggtattgg gcctggggct ggcggcggtg ctattcctgc gaggcaccgc
    11281 ccgaagctcc gccaatggag aatcacattg agcgatccgc gaaccgtagt cgcgcccgtc
    11341 acggccgcac tggcattgct gctggcggcg gggaccttga gcggctgcgc aaccagccct
    11401 cgctatggtc ggcccgcaga tcccgtgcaa actcagtatc tcgacccggc aaatttgccg
    11461 cccgtccaaa ccgccgcacc cgtgccgcac gtatccatgg gcaacggttc tgcggagcgc
    11521 tggtggactc tgcttggttc gccggacatc gatcggctgg tgacgctggc gctgcagaac
    11581 aatcgatcgc ttgccaccgc gaacgcgcat ttggcggcgg cacgcgagca tctacgcgcc
    11641 gcgcgcggcg cgtggtatcc ccaggtggac gcgggcgcgg gtgcgcagca cacccggttc
    11701 ggggccactt cgctaggccc gttggccaag gactttccgc ccttctctgc ctatgccgcc
    11761 ggcgtcgagg tcagttacga cttcgatttc ttcggcagca cgaggtcgcg cgtcgaacag
    11821 gccgccgcct ccgcgcagta cgaatcggca cagctagagg ctgccgcgtt gagcgtcagc
    11881 ggaaacgtcg tcctcgaagc gctgcaggtt gccgcattgc gcgcacagat ccgggttgcg
    11941 gagcagatcg tggccgacga tgaacacatg ctgggcctga ttcgcgccgc gcgcgaggca
    12001 ggcgcggtat cgcagatcga tgtgctgagc tcacagagtc aaaccgacca cgatcggact
    12061 ctgctgccgc cgctgcgaca gcagttgagc gcggcgcagg atgtgctggc ggtgctgctc
    12121 ggggcagccc ccgccgatgg gacgccgcgc gaagtggagc ttggtacgct ccggccggcg
    12181 gatgatttgc cggtggcgct gccttccgaa ctcgtgcgcc gccggcccga catcgacgcg
    12241 gccgaggcgc agctccatga agccggcgcc gcagtgggca tcgcgacggc gaacctgtat
    12301 cccaatttca cattgagcgc ccgcctggct ggggaaggcc tgctcaacgg cggcccgtcg
    12361 gaaactgcat ggaatctcct gggagggctg acggccccaa tttttcacgg cggcgcgctg
    12421 agcgcagagc ggcgggccgc acaggatgac tacgaggcgg cctttgcggc ctaccagcaa
    12481 accgtgctga atgcgttcgg ccaagtcgcc gctgccctgc aggcgctcgt caacgacgcg
    12541 gaattgttga gtgcgcagca gcgtgccctg gattctgcgg gcgcgtcatt gactctgacc
    12601 cggcaagcct ataaggcagg caacgctggc tacacgcaag tgctggatgc cgagaggctg
    12661 cacagccaag ccgagctcgg ccgcgttcag gccgatgggc agcgctatat cgatacggcc
    12721 aagttgctgt tggccgccgg cggccgcagc ggtgttgcgc cgaacgacgg gtcgcgtctc
    12781 acgtttcatc cgtaacggaa ttcaatcgtg gaagtttcaa tggaaaccgc agcgacattg
    12841 atttcgagcg cggcgttgtt agctttttct cgaggtagat tttggattcg tgcgtccaca
    12901 tcctacggag gctgataaat gctctcttcc cgtcggtgca gatatcgcgg atcggtgctg
    12961 gctgttttcg ccgccctgtc cgcaggcagt tcacaggcct ccccggaggc gccagccgcg
    13021 gccccacaac ccactgtggc cgcatgcggc gcaggcggga cgggtattgg gctgtacaaa
    13081 ggcgccctgt tcgcggagat caacgacaag atcgtgcgtt attcgctgcg cgcagggtcg
    13141 atcgtcccgc aaggtcccgc cgtaccggtg gtgaccggct tgccgctgag cggggatcat
    13201 cccatgcatc cattcaccat cgatggcgat ggtttcctgt tcatcgacgt cgctaccccg
    13261 accaacgcct gccagcggaa gaatcgcacg ttgaaatcgc cgggcatcgt cccgtgccca
    13321 gagctcgaga cgcgcggcgg catttggcgg tatgacgcga acaaagcaaa tcaggcattc
    13381 tcgccggcgg aacgctttgc cacgggcatc cgcaatgcgg aaggattcgc gatcgacgcc
    13441 gccggacgca tgttcgtcac acaacatgga cgagaccagc tgcacgcgaa ttggccggat
    13501 ctgtaccagc cccgaggagg aggctacgca gcccgctgaa gagttgctgc tcttgagaca
    13561 gggcggcgac tacgggtggc cggagtgcta ttacgacggc gttcagggaa aattggttct
    13621 cgctccggag tacggcggcg acggcggcaa gacccaggga gtgtgcg
//
LOCUS       DASWZT010000425         4881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_51639, whole genome shotgun sequence.
ACCESSION   DASWZT010000425 DASWZT010000000
VERSION     DASWZT010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51639"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VGE92_12180"
     CDS             complement(<1..715)
                     /locus_tag="VGE92_12180"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY1314632.1"
                     /translation="MSGPTPRLSRRAALGVLAGSATVLAASQTASPENAASAGRLKQS
                     FSHWPYAKTPLPEVCRRAKQIGFGAIDLLFPDEWPTAREAGFTVSMGYASRRDKFIAT
                     GFNDPANHAMLQDELAGALPLAARAKVPNVIAMFGNRVAGISEEAALANCVNGLGKIA
                     PLAAEHGVTVCVELLNSKVDHPGYQGDHTAFGVAVMKGINSPHVKLLYDIYHMQIMEG
                     DVIRTIRDNIRWIGHFHTGG"
     gene            complement(722..1630)
                     /locus_tag="VGE92_12185"
     CDS             complement(722..1630)
                     /locus_tag="VGE92_12185"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEY1314633.1"
                     /translation="MQSMSRREFLLRTSALAATLAVGGFARRAYATPMGRPAGIQLYA
                     VGSALQENPAATLKRIKDIGYGEVETAGFARLSAKEFRKLLDDAGLKCPSGHLQFDLA
                     HLGPTFEDAHTLGMHYVASGGLRGSLSPPITGPMSGMSLDEAKRTAELANQIGAKAKQ
                     AGLQYAYHNHNIEFADHGGVVGYDILLRDTDPKLVKFEIDCGWMVLGNRNPVDYFKKY
                     PNRFPMIHVKDFLPPPDQGEAVGRSTENHAGAELGHGMIDYRPIFAAAEKAGLQHYFV
                     EQEGPFVRMNQLQAAEQAYAYLSAIG"
     gene            1774..3450
                     /locus_tag="VGE92_12190"
     CDS             1774..3450
                     /locus_tag="VGE92_12190"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY1314634.1"
                     /translation="MQSANYDAIVVGSGISGGWAAKELTEKGLKVLLLERGRNVEHIK
                     DYVNATKAPWEYLHRGRRTYAMEQAYQVLRRDLLNETNLAFWVDEKESPYTEVKRFDW
                     FRGYQVGGRSLLWGRQSYRWSDFDFEANLKEGIASDWPIRYNELAPWYDHVEKHIGVS
                     GSVEGMAQLPDGQFQPAMPLNCGEERIAGRLKSGFGGKRRIIIGRTANATRALEGRAP
                     CQYRNACWLGCPYGAYFSTQSSTLPAAAKTGNLTLKPWSIVTEILLDKDRKRASGVRV
                     LDAVTEQTTDYTAKVVFICASTLNSTWLLMRSATDVWPGGLGSSSGELGHNLMDHHFR
                     CGAEGRLEGLDDKYYYGRRPNGVYIPRYRNLFGDKRDYLRGFGYQGSAGREGWMRGVK
                     ELGVGAAFKDEMALPGPWTFGATGFGEMLPNHANRVALDTTRKDKWGLPVLKIDCAFG
                     ENERLMRKDMMSDLAEMLEHAGARNVDTYDHGSYPGMAIHEMGTARMGRDPKSSVLNA
                     HNQVWDAPNVFVTDGACMASTACQNPSLTYMAITARAADFAVNELKRRNL"
     gene            3456..4103
                     /locus_tag="VGE92_12195"
     CDS             3456..4103
                     /locus_tag="VGE92_12195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEY1314635.1"
                     /translation="MNPSAESAALLKVNRREAIRRVSALLGGIAFVGGSRLLAALDQP
                     NTAIDAAYGKFSAEDIAFLDEVAETILPATKTPGAKAAKTGAFIAVMVTDTYNPAQQK
                     VFREGMRKVDEAAQANQVSFMAATPEQRLAVLDKLDREQKRVMDARAAADRHKPAGED
                     PEPPHYFRMIKELTLLGYFTSEVGCTQALRYMETPGRYDACLPYAPGEPAWASHA"
     gene            4189..>4881
                     /locus_tag="VGE92_12200"
     CDS             4189..>4881
                     /locus_tag="VGE92_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314636.1"
                     /translation="MRFSSRFSAWRAYSGPIFVIAALIAPSVIAVSDDPSNVARTLFA
                     CGVSIGLASAALALYHRPWMRRLLHLGLFLILLELFYRFAYGGAVSSGLLLSIPETSG
                     RETLELLAGHPILTASLSLVAILAVLTLILSWNGSVRIALRRCAQAAVLALAMVIASL
                     AFGPSQVGRPRAAVAQVEAVFPLDIAAAFGAVAGDWIDAHLQASRRAAFKFPNARLVD
                     AAAREGAAEGYVG"
BASE COUNT          897 a         1517 c         1552 g          915 t
ORIGIN      
        1 ccccgccggt gtgaaaatgc ccgatccagc ggatgttgtc acggatcgtg cggatcacat
       61 cgccttccat gatctgcatg tgatagatgt catacagcaa tttgacgtgc ggtgagttga
      121 tgcctttcat cacggccacg ccgaaagccg tgtgatcgcc ctggtagccg ggatgatcga
      181 ccttgctgtt gagcaattcc acacagaccg tgacgccgtg ctcggcggcg agcggcgcga
      241 ttttgccgag cccgttgacg cagttcgcaa gcgcggcctc ttcgctgatg cccgccaccc
      301 gattgccgaa catggcgatc acattgggca ccttcgcgcg cgctgccagc ggcagcgcac
      361 ccgccagttc atcctgcagc attgcgtgat tcgcgggatc gttgaacccg gtcgcgatga
      421 atttatcgcg ccggctcgca tagcccatcg agaccgtgaa gccggcctcg cgcgcggtcg
      481 gccactcatc gggaaagagc aggtcgatcg caccaaaacc gatttgtttc gcgcggcgac
      541 acacctccgg cagcggcgtc ttcgcataag gccagtgcga gaaggattgc ttgagacgcc
      601 ccgcagaggc ggcattctcc ggcgacgcgg tttgcgatgc ggcgagaacg gtggcgctgc
      661 ccgcgagcac gccgagcgcg gcgcggcggc tgaggcgtgg ggttgggccc gacatggcgc
      721 gctaaccgat cgcgctcaaa taggcatacg cttgctcagc cgcctgcaat tggttcatgc
      781 gcacgaaagg tccttcttgc tcgacgaaat agtgttgcaa gccggccttt tccgctgccg
      841 cgaaaatcgg ccggtagtcg atcatgccgt gtccgagttc cgcgcccgca tgattttcgg
      901 tcgatcggcc gacggcctca ccctgatcgg ggggcggcaa gaaatccttg acgtgaatca
      961 tcggaaatcg attcggatat ttcttgaagt agtcgaccgg gttgcggttg cccaatacca
     1021 tccagccaca atcgatctcg aacttcacga gcttaggatc cgtgtcgcgc agcaagatgt
     1081 cgtaaccgac gacgccgccg tgatcggcaa actcgatgtt gtgattgtga taggcatact
     1141 gaaggccggc ttgcttggcc tttgcgccga tttgattggc caattctgcg gtacgcttcg
     1201 cctcatcgag gctcattccg gacatgggtc ccgtgatcgg cggggacaag ctgccgcgca
     1261 atccgccgct cgccacgtag tgcattccga gcgtgtgtgc atcctcgaaa gtcggcccca
     1321 aatgggcgag gtcgaactgc aggtggccgc tcggacactt cagtcccgca tcatcgagga
     1381 gcttgcggaa ttccttcgcc gataacctgg caaagccggc cgtctcgact tcgccgtagc
     1441 cgatgtcctt gatgcgcttg agggtcgccg ccggattctc ctgcagggcg gaacccacgg
     1501 cgtagagctg gatacctgcc ggccgaccca tcggcgtcgc gtaggcgcgg cgcgcaaagc
     1561 cacccacggc caacgtcgcc gcgagcgcac tcgtccgaag cagaaattct ctccgggaca
     1621 ttgattgcat gatgatcgac tccccacatg cgccgtacga gcgccgcccc gcgattacga
     1681 tattacattt ccggaaacga gttagaatta atcggctggc ggccggcgtc gcgagaccga
     1741 aagacgagtg atgggggagg ggctttggca tcgatgcagt ccgcgaacta cgacgcgatt
     1801 gtggtcggct ccggcatatc cggcggctgg gctgccaagg agctcactga gaaggggctc
     1861 aaggtgctgc tcctcgagcg tggccgcaac gtcgagcaca tcaaggatta cgtcaacgcg
     1921 accaaggcgc cctgggagta cttgcatcgc ggccggcgca cgtacgccat ggagcaggcc
     1981 taccaggtgc tcaggcggga tctgctcaac gagacgaatc ttgcgttctg ggtcgatgag
     2041 aaagaatcgc cttacacaga agtaaagcgc ttcgattggt ttcgcggcta ccaagtgggc
     2101 ggccgatcgc tgctgtgggg acgccagagt taccgctgga gcgacttcga ttttgaagcc
     2161 aacctcaaag agggcatcgc gagcgactgg ccgattcgct acaacgaact cgcgccgtgg
     2221 tatgaccatg tcgaaaagca catcggcgtc tcaggatccg tcgaaggcat ggcgcagctt
     2281 cccgacggac aattccagcc tgcgatgccg ctcaactgcg gtgaggagcg gattgccggc
     2341 cgattgaaga gcggattcgg cggcaagcgc cgcatcatca tcgggcgcac cgcgaacgcc
     2401 acgcgcgcgc tcgaagggcg cgcgccgtgc cagtaccgaa atgcctgttg gctcggttgc
     2461 ccgtacggcg cctatttcag cacccagtcc tcgacgcttc ccgcagccgc gaagaccggg
     2521 aatctcacgt tgaaaccctg gtccatcgtg accgagattc tgctcgacaa ggatcgcaag
     2581 cgggcgagcg gcgtgcgcgt gctcgacgcc gtgaccgagc aaaccacgga ctacacggca
     2641 aaagtggtct tcatctgcgc ctcgacgctc aactcgacct ggctcctgat gcgaagcgcc
     2701 acggatgtgt ggccgggcgg actcggcagc agctcaggcg aactcggcca caacttgatg
     2761 gatcatcact ttcgctgcgg cgccgaggga cggctcgaag gcttggacga caagtactac
     2821 tacggccgcc ggccgaacgg tgtgtacatc ccgcgctacc gcaacctgtt cggcgacaag
     2881 cgcgattacc tgcgcggctt tggctaccag ggcagcgcgg gccgggaagg gtggatgcgc
     2941 ggagtcaagg aattgggtgt gggcgcggca ttcaaggacg agatggcgtt gccggggccg
     3001 tggacgttcg gcgccacggg tttcggcgag atgcttccca accacgccaa tcgtgtggcg
     3061 ctcgatacca cccgaaagga caagtgggga ttgccggtcc tcaagatcga ttgcgccttc
     3121 ggcgagaacg aacggttgat gcgcaaggat atgatgtctg acctcgccga gatgctcgag
     3181 cacgcgggcg cccgcaacgt cgacacctat gaccacggtt cctatccggg catggccatc
     3241 cacgaaatgg ggacggcgcg catgggccgc gatccgaaga gctcggtact gaatgcgcac
     3301 aaccaagtgt gggatgcgcc gaacgtgttc gtcaccgacg gcgcctgcat ggcctcgacc
     3361 gcctgtcaga atccctcgct cacgtacatg gccatcaccg cgcgtgccgc ggattttgcg
     3421 gtgaacgaac tcaagcggcg aaacctctga tgcctgtgaa cccctcagct gaatccgcag
     3481 cgctgctcaa ggtcaatcgc cgcgaggcca ttcgccgggt gagcgcattg ctcggcggga
     3541 tcgcctttgt gggcggcagc cgactgctcg cggccctcga ccagccgaac accgcgatcg
     3601 acgctgctta tggaaaattc agcgccgagg atattgcctt cctcgatgaa gtcgccgaga
     3661 ccattctgcc ggcgaccaag acccccgggg caaaagccgc caagacgggc gcgttcattg
     3721 cggtcatggt caccgacacc tacaatcccg cacagcaaaa ggtgtttcgc gagggcatgc
     3781 gcaaagtgga cgaggccgcc caggcgaacc aggtttcctt catggcggca acgccggagc
     3841 aacgactcgc ggtgctcgac aagctcgacc gcgagcagaa gcgggtgatg gatgcgcgcg
     3901 ccgcagcgga tcggcacaaa cccgccggcg aggaccccga gccgccgcac tactttcgca
     3961 tgatcaaaga gctcaccttg ctcggctact tcacctcgga ggtgggttgc actcaggcgc
     4021 tgcgttatat ggagaccccc ggccgctacg atgcctgtct tccgtatgcg cccggcgagc
     4081 ccgcctgggc gtcgcacgct tgaatgcgcg cgtgaattaa cgtgaaccaa cccgggtagg
     4141 cgcaagtccg aacttgccca tccgtatcgg ggcgtttacc gtagccgcat gagattttcg
     4201 tcgcgtttca gcgcctggcg ggcttacagc ggcccgatct tcgtcattgc agcactcatt
     4261 gccccgagtg tcatcgccgt gagcgatgac ccctccaacg ttgcgcgtac gttgttcgcg
     4321 tgcggggtat cgatcggcct cgcgagcgcc gccctcgcgc tctatcaccg gccgtggatg
     4381 cggcggttgc tgcacctcgg cctgtttttg attctgttgg aactctttta tcgctttgcc
     4441 tatggcgggg ccgtttcttc ggggctgctg ctgtcgatac cggaaaccag cgggcgcgag
     4501 actctcgagt tgctcgcagg tcatccgatc ttgacggcga gtctgtcgtt ggtcgcgatc
     4561 cttgccgtac tgacgctgat cctatcctgg aatggcagcg tgcggattgc gctgcgacgc
     4621 tgcgcgcaag cagccgtgct cgcgctcgcg atggtgatcg cctcgctggc gttcggcccc
     4681 tcgcaggtcg gcaggccgcg agcggcggtt gcgcaggtcg aggccgtctt tccgctggat
     4741 attgcggcgg cctttggcgc agttgcgggc gattggatcg acgcccattt acaggcctcg
     4801 cgccgcgccg cgttcaagtt cccaaatgcg cgcctggtgg atgcggctgc gcgcgagggc
     4861 gcggcggagg gttacgtggg g
//
LOCUS       DASWZT010000426         2019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_85511, whole genome shotgun sequence.
ACCESSION   DASWZT010000426 DASWZT010000000
VERSION     DASWZT010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85511"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="VGE92_12205"
     CDS             <1..1040
                     /locus_tag="VGE92_12205"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEY1314637.1"
                     /translation="AAAVLLIHDGEALGYGWKVVVNKWTGPQLQLASTEPRAAIEGWL
                     QKDAARALFNAAGHDFAAATAAAAQAGFKAIPLGVQADASLHSSLRAFSSANVIALWP
                     GAQKRKESVIYTAHWDHLGRDPTLPGHNIYNGAVDNASGVAGLIALAQSFIRTKPVAE
                     RSIVFLALTGAESGLLGSEYYVQNPVFPLRSTTAVLNLDTLRDRGPTRDVTIFDSGNT
                     DLEEYARAVALLQGREVTADPTPAQGRYYRSDNLSFAEAGVPALYVQGGLDDSARGPA
                     SGRSQLEDYMLHRYRQPSDQYSPDWDVRGALEDLTLYYEVGNRVARTRRFPRWYPNSE
                     FRVSREHRAAP"
     gene            complement(1044..1241)
                     /locus_tag="VGE92_12210"
     CDS             complement(1044..1241)
                     /locus_tag="VGE92_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314638.1"
                     /translation="MSSNDKLWNELAASFSYGASEVHGGTTRKLGAVAQPKAKENRGF
                     DPYNSSGNFDRKKNWNRVGKR"
     gene            complement(1407..>2019)
                     /locus_tag="VGE92_12215"
     CDS             complement(1407..>2019)
                     /locus_tag="VGE92_12215"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY1314639.1"
                     /translation="RSLHALREINTLRVAVEDNSSRIQEVEEETRRDDLTGVYNRAYL
                     DQFLSREFDNSTRHKWPLSVAFADLDNFKEINDNFGHQAGDRILQATARLLRGNTRET
                     DLVARYGGEEFVVVLPATDAETARSICERIVMAFRNTGHVIGSDHAKVTISIGCATHG
                     SQTLFTDVTDFIKAADQALYTAKMRGRNCSVPFDRQLSGARLA"
BASE COUNT          361 a          628 c          637 g          393 t
ORIGIN      
        1 gtgcggcggc cgtgctgctc attcatgatg gcgaggcgct cggctacggc tggaaagtgg
       61 tcgtcaacaa gtggaccggt ccgcaattgc agctcgccag caccgagccg cgagcagcca
      121 tcgaaggatg gctgcaaaaa gacgcggcgc gtgctttgtt caacgccgcg ggccacgatt
      181 ttgccgcggc aactgcggcg gcggcgcaag cggggttcaa ggcgattccc ttgggcgtgc
      241 aggccgacgc gagcctgcat tcgagtctgc gcgctttcag ttccgccaat gtgattgcgc
      301 tgtggcccgg cgcgcaaaag cgcaaggaaa gtgtcatcta cacggcgcac tgggatcatc
      361 tgggccgcga cccgacgctg ccgggtcaca acatttacaa cggcgccgtc gacaacgcca
      421 gcggcgtggc gggcctgatc gcgctggcgc agtcgttcat ccgcaccaaa cccgttgccg
      481 aacgcagcat cgtgttcctg gcgctcaccg gcgcggagtc cgggctgctc ggatccgagt
      541 actacgtgca gaatcctgtg ttcccgctgc gctcgacgac ggcggtgctg aatctcgata
      601 cgctgcgcga tcgcggaccc acgcgcgacg tcaccatatt cgattcgggc aatacggacc
      661 tcgaggagta cgcccgcgcg gtagcgctat tgcaggggcg cgaggtgacc gccgatccga
      721 ccccggcgca gggacgctac tatcgctccg acaatttgag cttcgcggag gcgggtgtgc
      781 cggcgctgta cgtgcaggga ggattggacg attctgcgcg cgggccggcg tccggccgtt
      841 cgcaactcga ggactacatg ctgcatcgat atcgccaacc cagcgaccaa tattcgccgg
      901 actgggatgt tcgcggcgcg ctcgaagatc tcacgctgta ttacgaggtc ggcaaccgcg
      961 tggcgcgtac gcgccgcttt ccgcgctggt atcccaacag cgagttcagg gtcagccgcg
     1021 aacatcgcgc ggcgccgtag cgttcagcgc ttgccgaccc tgttccaatt cttcttgcgg
     1081 tcgaaattgc ccgacgagtt gtagggatcg aagccgcggt tctctttcgc tttcggttgc
     1141 gcgacggcac ccaacttgcg ggtggtgccg ccgtgcacct cgctcgcacc gtagctgaag
     1201 ctcgccgcca gttcattcca taacttgtca ttgctcgaca tcaccaccct ccgcagattt
     1261 aaatcgtccc gtcaaccgtg cctgcttcgt tttgaatttt ttccttgccg acatgttccc
     1321 aaatcgcgac aaaaaaaggc tgacggcatc gcaaatctgt gacgcaactc caagtcgaaa
     1381 ctgacataag gccgggaagc ctgaagctat gcgagccgcg ccccggatag ctgccggtcg
     1441 aagggcacgg agcaattgcg gccgcgcatc ttggcggtgt agagggcctg gtccgcggcc
     1501 ttgatgaaat cggtcacgtc cgtgaacagg gtctggctgc catgggttgc gcagccgatg
     1561 gaaatcgtca ccttggcgtg atccgagccg atgacgtgcc cggtgttgcg aaacgccatg
     1621 acgatgcgct cgcaaatgct gcgtgccgtt tcagcatcgg tcgcgggcaa caccaccacg
     1681 aactcctcgc cgccgtagcg ggcgaccaaa tccgtttcgc gcgtattgcc gcgcagcagc
     1741 cgtgcggttg cctgcagaat acgatcgccg gcctggtggc cgaagttgtc gttgatctct
     1801 ttgaaattgt cgagatcggc gaacgccacg ctcaacggcc atttgtggcg ggtcgagttg
     1861 tcgaattccc ggctcaagaa ctggtcgaga taggcgcgat tgtaaacacc cgtcagatcg
     1921 tcgcggcggg tctcttcttc gacctcctgg atgcgcgagg agttgtcctc cacggcgacg
     1981 cgcaatgtgt tgatctcgcg cagggcgtgc aggctgcgc
//
LOCUS       DASWZT010000427         2564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_71078, whole genome shotgun sequence.
ACCESSION   DASWZT010000427 DASWZT010000000
VERSION     DASWZT010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71078"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="VGE92_12220"
     CDS             <1..1307
                     /locus_tag="VGE92_12220"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY1314640.1"
                     /translation="ARAAAARMDSVAPLAGVPFLAKDMNIDVAGLHLTASCRWLAELP
                     AATLDAPLAARWRSSGLSLLGRTNTPEFAGEFVTEPTWRGATQNPWDLGRSPGGSSGG
                     AAAAVASGMVPIAHGTDSGGSIRVPAAACGLVGLKPSRGWVPVGPWHDELAGGLDCEH
                     VLTRSVRDSAVMLDLTSGIEPTSRYALTRPAQPFAAAAAHASLPQLKIGMTLQAPGGI
                     LPLEEIGAAVEQAATALRRAGHAVSEFRFPRSANIGEPAGLIWLTAIAEEVDHYRTLV
                     GRDPRAHELEALTEASVALGRRSSAVDYVRARRALTAATRDMYVACRGFDLLLLPTTA
                     NVAPRTGQIDGRTAAFDLERWNQDSYGYAPYTEIFNVTGQPAISLPLALSSGGLPIGV
                     QLAAPLGEDARLLALAAWFEREQPWQSRLLALRRGFLDRGAG"
     gene            complement(1318..>2564)
                     /locus_tag="VGE92_12225"
     CDS             complement(1318..>2564)
                     /locus_tag="VGE92_12225"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF002825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HEY1314641.1"
                     /translation="TATWRRLSADRRYRTLSGEDLVLSGRSLMLVRNVGHHMMSDAVT
                     LDGKPIPETFIDAAVTSLAALHDLKGAGAGRNSRSGSIYIVKPKMHGPEEVAFAGDLF
                     AAVEDLLQLPRHTLKIGIMDEERRTTVNLTECIRAARHRVVFINTGFLDRTGDEIHTS
                     MNAAPVLRKNELKKAIWLDAYERQNVDVGLACGLRGRAQIGKGMWAMPDLMAGMLDAK
                     IAQPRAGANTAWVPSPTAATLHALHYHAVDVAQVQAALAGRARASLDEILTPPLVAHP
                     DWSAADIDEELNNNAQSILGYVVRWIDSGIGCSKVPDYYNVALMEDRATLRISSQHVA
                     NWLHHGVCSRDQVMRALIRMAGVVDRQNAAEPGYRPLADDPHHNIAFQAACDLIFKGR
                     EQPNGYTEAILHARRLERKALA"
BASE COUNT          392 a          836 c          869 g          467 t
ORIGIN      
        1 gcgcccgagc tgctgcggcg cgcatggatt cagtggcgcc gctcgcgggc gtgccgtttc
       61 tcgccaagga tatgaatatc gacgtcgccg gcctgcattt gacggcctcg tgccgttggc
      121 ttgcggaatt gcccgccgca acgctcgatg cacccctggc ggcgcgttgg cgcagctccg
      181 gcctgtcgct gctcggccgc acgaacacgc cggaattcgc gggcgaattc gtcaccgagc
      241 cgacgtggcg cggagcaacg cagaacccgt gggatttggg ccgcagcccc ggcggctcga
      301 gcggcggcgc ggcggcggcc gtcgccagcg gcatggtgcc catcgcgcat ggcaccgatt
      361 ccgggggttc gattcgggtt cccgcggcgg cttgcggact cgtgggcttg aagcccagcc
      421 gcggctgggt gcccgtggga ccttggcacg atgagttggc cggcggtctc gattgcgagc
      481 acgtgttgac acgctcggtg cgcgattcgg cagtgatgct cgatctcaca agcggcatcg
      541 agccgacctc gcgctatgcg ctcacgcgtc ccgcgcagcc cttcgccgcc gccgccgccc
      601 acgcgtcgct gccgcagctg aagatcggca tgaccctgca agcaccaggc ggaatcctgc
      661 cgctcgagga gatcggcgcg gccgtggaac aggccgccac ggcgctgcgg cgcgcgggtc
      721 atgcggtgag cgagtttcga tttccacgct cggcgaacat cggcgaaccc gcggggctca
      781 tttggttgac ggccatcgcc gaggaggtcg accactaccg caccctcgtg gggcgcgatc
      841 cgcgggcaca tgaattggag gcgttgaccg aagcgagcgt ggcgcttggc cggcgctcga
      901 gcgccgtcga ctacgtgcgc gcgcggcgcg cactcaccgc ggcaacccgc gacatgtacg
      961 ttgcgtgccg cggtttcgat ctgctgctgc tgccgacgac ggccaacgtg gcgccgcgca
     1021 ccgggcaaat cgacggtcga accgccgcct tcgacttgga gcgttggaat caggactctt
     1081 acggctacgc gccctatacg gagatattca atgtcacggg gcagccggcg atttccttgc
     1141 cgctcgccct gtcgagcggc ggactgccga ttggggtgca gctcgcagcg ccgctgggcg
     1201 aggatgcacg gctgctggcg ctcgccgcgt ggtttgagcg cgaacaaccc tggcaatccc
     1261 gcttgttggc gttacgccgc gggtttcttg accgcggcgc cggttgatcg ccgccgttta
     1321 cgccagcgcc tttcgctcca gccggcgcgc gtgcaaaatc gcttcggtgt aaccgttggg
     1381 ctgttcccgc cccttgaaga tcaaatcgca cgccgcttga aatgcgatgt tgtggtgcgg
     1441 atcgtcggcg agggggcgat agccgggttc cgcggcgttt tgccggtcga caacgcccgc
     1501 catgcggatg agcgcccgca tcacttggtc gcgcgagcac acgccgtggt gcaaccaatt
     1561 ggcgacgtgc tggctggaaa tgcgcaatgt ggctcgatct tccatcaggg cgacgttgta
     1621 atagtccggc actttcgagc agccgattcc cgaatcgatc cagcgcacga cgtatcctaa
     1681 gatgctttgg gcgttgttgt tgagttcttc gtcgatgtcg gcggcgctcc agtccggatg
     1741 ggcaaccagc ggcggcgtca gaatctcatc gaggctcgcg cgcgcgcgcc ccgcgagagc
     1801 tgcctgcact tgggcgacgt ccacggcgtg gtaatgcagg gcgtgcagag tcgcggcggt
     1861 gggcgagggc acccacgccg tattggcgcc ggcgcgtggc tgcgcgatct tggcatcgag
     1921 catgccggcc atgaggtccg gcatcgccca catgcctttg ccgatctggg cccggccgcg
     1981 caggccgcag gcgagcccga cgtccacatt ctgccgctca taggcatcga gccagatcgc
     2041 tttcttgagc tcgttcttgc gcagcacggg cgccgcattc atcgatgtgt gaatctcgtc
     2101 gcccgtgcga tcgagaaaac cggtgttgat gaacaccacg cggtgacggg ccgcgcgaat
     2161 gcactcggtc aaattgacgg tggtgcggcg ttcctcgtcc atgattccga ttttcaaagt
     2221 gtgccgcggc agttgcagca gatcttccac cgctgcgaac aagtcgccgg cgaacgccac
     2281 ctcttcgggt ccgtgcattt tgggtttgac gatgtagatc gagcccgagc gggaattgcg
     2341 gccggcgcct gcgcctttaa gatcgtgcaa cgccgcgagc gaggtaaccg ccgcatcgat
     2401 aaacgtctcg ggtatcggct tgccgtccaa cgtcactgca tccgacatca tatgatgacc
     2461 gacgttgcgc accagcatga gactgcggcc ggagagcacg agatcctcgc cgctcaacgt
     2521 gcgatagcgt cgatccgcgc tcaggcgccg ccacgtggcg gtgc
//
LOCUS       DASWZT010000428         3242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_13228, whole genome shotgun sequence.
ACCESSION   DASWZT010000428 DASWZT010000000
VERSION     DASWZT010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13228"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VGE92_12230"
     CDS             <1..384
                     /locus_tag="VGE92_12230"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY1314642.1"
                     /translation="PLTRIIVSPYTRALQTAQPILDWHQVPVTIMHEVREQAAFTCDI
                     GSAPTVLASRFPHHDFEHLPAKWWHEGTETSAQTRERANGFRVLMAAREDKDTTLLVS
                     HWAFLLALCGIELANGEILEYDPLA"
     gene            complement(405..2528)
                     /locus_tag="VGE92_12235"
     CDS             complement(405..2528)
                     /locus_tag="VGE92_12235"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEY1314643.1"
                     /translation="METTTSTRITASERLHAAFAAVRALDLAGADPGLRRTALDVAEI
                     VLALDADDEVVIAAMLQPLLDAKLLDREAAARNFGADATRLARNLSQLGEFGLSVDWQ
                     PEQGLESGQAEALRKMLLAVIGDVRLVVVRLAAQLQKMRAAKSLAEVEQRKLATETRE
                     VYAPLANRLGVWQLKWELEDLAFRYLQPADYKRIAAALKVRRAEREHYMEELKALLQR
                     ELRHAGVDATIEGRPKHIYSIWRKMQAKQLAFEQLMDIRAARVLVNSVAECYAALGVV
                     HSLWQFIAGEFDDYIATPKDNRYRSIHTAVIGPGAQAVEIQIRTHEMHADSERGVASH
                     WRYKEGGRGDQAYERKINQLRSLLAPGESGGSSRDFLDRMRVDLFQDRIYVVSPKGEI
                     VDVPVGGTPLDFAYQVHTDLGHRTRGAKVNGRMVGLDYALKNGETVEIITAKTGQPSR
                     DWLSPQSGFLASPRHRNKVRAWFRKQNETDNKAEGRALFDREIQRLGINSPPIPELLG
                     ELKLPSAEALHEALGLGDVSSAQVAGAIQRILHAREARPEYVARARGTAREPDVEVQG
                     IGDLLSTFARCCKPVPPEGIVGYITVGRGVSIHSQSCANLARLQIKAPARVLAVSWGK
                     MAANEFPVDIEVQAFDRRGLVRDVSAALADDKISIQGMNTVIDKRDNVAHMHIAISIT
                     GLPQLSQVLAHIAQLPNVISARRKR"
     gene            2659..3183
                     /gene="ruvC"
                     /locus_tag="VGE92_12240"
     CDS             2659..3183
                     /gene="ruvC"
                     /locus_tag="VGE92_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004752762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY1314644.1"
                     /translation="MHGASTASVRVLGIDPGSQRTGFGVLDAVGPRLTYVASGVIRTQ
                     KGEFADRLCEIFRCVQTLVAQYRPHEIAIERVFVNRNPDSALKLGQARGAAICGTADA
                     NAEVFEYATRQIKQAVVGSGSAEKAQVQLMMKSILKLDGPLSADAADALAAAVCHALR
                     GRVRVSVLRAASRG"
BASE COUNT          566 a         1038 c         1013 g          625 t
ORIGIN      
        1 ccgctgacgc gcatcatcgt ctcgccgtac acacgggcgc tgcagaccgc acagcccatt
       61 ttggactggc atcaggtgcc ggtgaccatc atgcatgagg tgcgcgagca agctgccttc
      121 acctgcgata tcggcagcgc gcccacggtg ctcgcaagcc ggtttccgca ccacgatttt
      181 gagcacctgc cggccaagtg gtggcatgag ggcactgaga cgtcggcaca gacccgagag
      241 cgcgcgaacg gatttcgggt tttgatggcc gcccgcgagg acaaggatac gactttgctg
      301 gtcagtcatt gggcgtttct tctggccctc tgcggcatcg agctcgccaa cggcgagatt
      361 ctcgagtatg acccgctggc gtgaggacgg cggcgaaatc cgctctagcg ctttctcctc
      421 gcgctgatga cgttcggcag ctgggcgatg tgcgccagca cctgcgaaag ctgcggcagc
      481 ccggtgatcg aaatcgcgat gtgcatgtga gcgacgttat cgcgtttgtc gatcacggtg
      541 ttcatgccct ggatgctgat cttgtcgtcc gccagcgccg cgctcacgtc gcgcaccagt
      601 ccgcgccgat cgaatgcctg cacttcgatg tcgacgggga attcgttcgc cgccatcttg
      661 ccccaggaaa ccgccagcac ccgtgccggt gctttgatct gcaggcgcgc caaattggcg
      721 caagactgcg aatgaatgct gacgcctcgc cccaccgtga tgtagccgac aatgccttct
      781 ggcggtaccg gcttgcaaca gcgcgcaaag gtcgacagca agtcgccgat gccctgaacc
      841 tcgacatccg gttcgcgcgc agtgccgcgc gcgcgggcaa cgtactccgg ccgcgcctcg
      901 cgggcgtgca gaatccgctg aatggctccc gccacctggg cggagctgac atcgcccagc
      961 cccagggctt catgcaaggc ttcggcgctc ggcaacttca gttcgccgag cagttctggg
     1021 attggcggac tgttgattcc caatcgttgt atctcgcgat cgaagagggc acggccctcg
     1081 gccttgttgt cggtctcgtt ttgcttgcga aaccaggcgc gcaccttatt gcggtgcctc
     1141 gggctcgcca aaaatccgga ctgcggggag agccaatccc gcgacggctg gccggtcttg
     1201 gcggtaatga tctcgacggt ttcgccgttc ttgagcgcat agtcgagccc taccatgcgg
     1261 ccattcacct tcgcgcctcg ggtgcgatga cccaaatccg tatgcacctg ataggcaaaa
     1321 tccagcggcg taccgccgac cgggacatcg acgatctcac ccttcggaga cacgacgtag
     1381 atgcgatctt gaaacagatc gacccgcatg cgatcgagaa aatcgcgtga tgagccgccg
     1441 ctctcgccgg gggccagcaa cgagcggagt tggttgattt tacgctcata ggcctggtcg
     1501 ccgcggccgc cctccttgta gcgccaatgc gaggccacgc cgcgctctga atcggcgtgc
     1561 atttcgtgcg tgcggatttg tatttccacg gcttgggcgc cgggtccgat gactgcggtg
     1621 tgaatcgagc gatagcggtt gtccttggga gttgcgatgt aatcgtcgaa ctccccggcg
     1681 atgaactgcc agagcgaatg caccacaccc agggccgcgt agcattctgc aacgctgttg
     1741 accaggaccc gggcggcccg aatgtccatc aattgctcga acgcgagctg cttggcctgc
     1801 atcttgcgcc aaatgctgta gatgtgtttg ggccggccct cgatcgtcgc gtccaccccg
     1861 gcgtgccgca actcgcgctg caacagcgcc ttgagttcct ccatgtagtg ctcgcgttcg
     1921 gcacggcgca ccttcagggc ggcggcgatg cgtttgtaat cggcgggctg caaatagcgg
     1981 aatgcgagat cctcgagttc ccacttcagc tgccacacgc ccagtcgatt ggcgagcggc
     2041 gcatagactt cgcgggtctc ggtggcgagt ttgcgctgct ccacctcggc caaagacttg
     2101 gcggcgcgca ttttctgcag ctgagccgcc agacgcacga ccaccagccg cacatcgccg
     2161 atgacggcga gcagcatttt gcgcagcgcc tcggcctgac ctgactcgag gccctgttcc
     2221 ggctgccaat cgacgctcaa accgaattct cccagctggc tcaagttgcg cgccagccgc
     2281 gtcgcatcgg cgccaaaatt ccgcgccgcg gcttctcgat cgagaagctt ggcatcgagc
     2341 agcggctgca acatcgccgc gatcaccact tcgtcgtcgg catccaaagc gagtactatt
     2401 tccgctacat ccaaggcggt gcgccgcaat cccgggtccg cgccggccaa atcaagcgcg
     2461 cgcaccgctg cgaaagcagc atgcagccgc tcactggccg tgatccgggt tgatgtcgtc
     2521 gtttccactt gaattgtgcc gcaggctgcc tggtgggttt aagcgcctat tgccggtatc
     2581 atactcgcca atgacagacc caaagaatgc agtctaacag cggccacccc ggcgcggccc
     2641 gcgcggtggg cgatgcgcat gcacggcgct agcacggcga gcgtgcgcgt gctcggcatc
     2701 gatcccggct cgcagcgcac cggtttcggt gtgctggatg cggtcggccc gcgtttgact
     2761 tacgtggcga gcggcgtcat ccgcacccag aagggcgagt tcgccgaccg gctgtgcgag
     2821 atttttcgct gcgtgcagac cctggtcgca cagtaccgcc cgcatgagat cgccattgaa
     2881 cgcgttttcg tcaaccgcaa tcccgacagc gcgctcaaat tgggacaggc gcgcggcgcg
     2941 gcgatttgcg gaaccgccga tgccaatgcc gaggtatttg aatacgcaac gcggcaaatc
     3001 aagcaggcgg tggtcggatc cggcagtgcc gagaaggcgc aagtgcagct catgatgaag
     3061 agcattctca agctcgacgg accgctgtct gccgatgcgg ccgatgcctt ggcggctgcg
     3121 gtgtgccacg cgctgcgcgg tcgcgtgcga gtctcggtac tacgcgcggc gagccgcggg
     3181 tgatcggttt tctcaaaggg cgtttgtcgg tcaagcagcc gccgatgctg atggtcgatg
     3241 tg
//
LOCUS       DASWZT010000429         4165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_71107, whole genome shotgun sequence.
ACCESSION   DASWZT010000429 DASWZT010000000
VERSION     DASWZT010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71107"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VGE92_12245"
     CDS             <1..235
                     /locus_tag="VGE92_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314645.1"
                     /translation="WMGKHQAAIVAMGGPLGKTKKVTQRGIEDTGNDLGAFMVVRAES
                     HEAAAKLFEKHPHFTIFPGESVEVMPVLPIPGG"
     gene            433..3618
                     /locus_tag="VGE92_12250"
     CDS             433..3618
                     /locus_tag="VGE92_12250"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEY1314646.1"
                     /translation="MNSWGERRDLCRRRFRRVAAHAGFAAVAAALISTASLAADGPDV
                     FAQLGHSNVVHSVAFSANGRLLASGGEDGAALVWDVATRREFRMLKLDSGVVDAVSLS
                     PDGALLAAGTHDGTVVIWEIATGRALHVLKGHSGAVNSVAFTSNGRMIASGSADHTVK
                     LWDAAAGTELKTLSSHRDAVTSVAFSSDDRMLASGSVDKTVKLWEVASGHEIKTLSGH
                     TDRVSSVAFCPAPSSVLASGSWDHAVKLWDASTGREVRTLTGHTSQVWSVACAKAGRV
                     ASGGYDHSVRLWDAATGQSLRTLAADAGWVESVSFSPDGRELASGGADHSVKLWSVAS
                     GEKLQTLEGHADFVKSLAFSANGRMLVSGGADRAVRLWLVADGHVLRQIPAHDNWVGS
                     VAFSPDNHAVASRSGDRTVKLWDIVTGRVLHTFSVGNAQSGSASIAISPDGHSLAAGI
                     ADNSIKLWRLPDGAELKTLAGHAASVEAVAFSADGRNLASGDARGSIRIWDVASGREQ
                     HTLSGHTSWVGSVAFSPDGRTLASGGGDKTLRLWDVQGGKEQHVLRGHEAAVTSLSFD
                     PKNPRLASSDESGIIKLWDLTGSRELRTLAGHSDLVESVAFSPDGMLLASASADSTIR
                     LWDVASGEERVRLIAFRGGSVLRITPQGYYDFQGDGAEEYLNVRAHGEVSGIEAYREK
                     FYRPDLVRLALNGRPMPAALPTIATVKPPPDVVIVDAPAQTDAKEIDLQVRITDRGGG
                     IGTVRTLVNGSAVSQVPGGPAAGSVQSRSIHVKLEPHGNDIRVVAFNTDGSVQSNAAE
                     ATVAANFDPSGKPQLHALVVGIQEFDVAGKNLEFSVADATAIAQVLEKKAAPLFSKVN
                     VQTLTTKATTTKAALMSAFERYRSIDPQDVFVFYAATHGSVEGDLANPEYYLITSNVK
                     DTTPEAIRRDTLSGTELRQLVASIPATRKLLLLDTCQSGAAGDAMMLTTRGLEENGAV
                     TVLSGAVGSTVLSASTREQSALEGQDGHGLFTWVLLHGLQGDADARHNGYIKTFDLAA
                     YVDDEVPKISQHKQNPNLHNAGQSFEIVSTQ"
     gene            3638..>4165
                     /locus_tag="VGE92_12255"
     CDS             3638..>4165
                     /locus_tag="VGE92_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314647.1"
                     /translation="MTSFQIPRTSRGIKGANGRTALTVLGALLSASPAGAQTTNTQQP
                     IKRPVVPVPQKPATGVQNNLFSAFGAANNNSAAHTAPVLSPQAQKFLNPNANTAAGNN
                     TPANRNPLAALVNPNGAANRQAPSNLNNLQRPVATGPQVPARSVPGLPAPRGAKEMQG
                     HNGSIIRTASDGSVLD"
BASE COUNT          779 a         1294 c         1363 g          729 t
ORIGIN      
        1 gtggatggga aaacatcaag ccgccattgt cgcgatgggc ggtccgctcg gcaaaaccaa
       61 gaaggttacg caacgcggga tcgaggacac cggcaatgac ctgggagcct tcatggtggt
      121 gcgcgccgag tcgcatgagg ccgccgccaa gcttttcgag aagcatccgc acttcacgat
      181 ttttccgggc gagtcggtcg aagtcatgcc ggtgttgccg ataccgggcg gttgatgatg
      241 gcagcgcgaa gaactcaatg ggagcgcgac acatcgttgc aatgagttcc taagcttcgc
      301 cctccggtca tcgccctgca gattatgtca ttgacttttc tgcataatat aggcgcgcat
      361 gcgcggttag agaaaacgct gcgcagcgcg tatcatcagt aaaaccaagg gacaaacgaa
      421 gagccgaatc tcgtgaattc gtggggtgaa cgaagggatc tgtgtcgacg gcggtttcgc
      481 cgcgtcgccg cgcatgcggg attcgccgct gtggcggcgg cgctcatctc cacggcaagc
      541 cttgccgccg acggacccga tgtatttgcc cagttggggc attcgaatgt cgtccactcg
      601 gtggccttct cggccaacgg tcgtctgttg gcctccggcg gtgaagacgg cgccgcactc
      661 gtctgggatg tcgccacccg gcgcgaattc aggatgctca aactcgactc cggtgttgtc
      721 gatgccgtca gtctctcgcc ggacggggcg ctgcttgccg cgggcaccca cgacgggacg
      781 gtggtgatct gggaaatcgc caccgggcgg gcgctacatg tccttaaagg ccactcggga
      841 gccgtcaata gcgtcgcctt cacgtcgaac ggacgaatga tcgcttcggg cagcgccgat
      901 cataccgtga agctctggga tgccgccgcc ggcaccgaac tcaagacctt gagctcgcac
      961 cgcgatgccg tgacgtcggt ggcattctcc tccgacgatc ggatgcttgc ctccggcagc
     1021 gttgacaaga cggtgaagct ctgggaggtc gcgtccggcc acgagatcaa gactttgagt
     1081 gggcacaccg atcgggtcag ttcggtggcg ttctgtcctg cgccgagttc ggtcctggcc
     1141 tcggggagtt gggatcacgc ggtcaagctc tgggatgcgt caaccggtcg cgaagtgcgc
     1201 acgctcacgg gccacacgag ccaggtctgg tcggttgcct gtgcgaaggc cggccgcgtc
     1261 gcctccggcg gctacgacca cagcgttcgg ttgtgggatg ccgctaccgg ccagtcgctg
     1321 cgtacgctgg ccgcggatgc gggatgggtt gaatcggtaa gtttctcgcc cgacggtcga
     1381 gaacttgcgt ccggcggcgc cgatcactcg gtcaagctct ggagcgtcgc gagcggcgag
     1441 aagctgcaga cgctcgaagg tcatgcggac ttcgtcaagt cgttggcgtt ttcggcgaac
     1501 ggccgcatgc tcgtctcagg cggcgccgac cgggcggtgc ggctatggct ggtggccgac
     1561 ggtcacgtgc tgcgccagat tccggcccac gacaactggg tgggatccgt ggcgttctcg
     1621 cccgacaatc acgccgtggc ctcgaggagc ggagatcgca ccgtcaagct ctgggacatc
     1681 gtcacgggcc gggtgctgca caccttttcg gtgggcaatg cgcaaagcgg cagtgcgtcg
     1741 atcgcgattt cacccgacgg ccatagcctg gccgccggaa tcgcagacaa ttcgatcaag
     1801 ctatggcgac tgccggacgg cgcggaactc aagacgctgg ccggacacgc ggcgtcggtc
     1861 gaagcggtgg cgttctcggc cgacgggcgc aacctcgcct ccggggacgc gcgcggctcc
     1921 atcaggatct gggacgtggc cagcggccgc gaacagcaca cgctcagcgg ccacacgagc
     1981 tgggtggggt cggtcgcctt ctctcccgac ggccgcacgc tggcatcggg aggcggcgac
     2041 aaaacgctgc gcttatggga cgtgcagggc ggcaaagagc agcacgtgtt gcgtggacat
     2101 gaggcggccg tcacatcgtt gagcttcgac cccaaaaatc cgcggctcgc ctcgagcgat
     2161 gagagtggga tcatcaagct ttgggatctt acgggttcgc gggaactcag gacgctcgcc
     2221 ggtcattctg atctggtgga atccgtggcg ttttcgcccg acgggatgtt gctcgcgtcc
     2281 gcgagcgcgg attcgacgat tcgactgtgg gacgtggcga gcggcgagga gcgcgtgcgg
     2341 ctcatcgcgt ttcggggcgg atctgtcctt cgcatcacgc cgcaaggtta ttatgatttt
     2401 caaggagacg gggccgagga atacctcaat gtccgcgcgc acggcgaggt gtccggcatc
     2461 gaggcctatc gcgagaaatt ctatcgcccc gatctcgtac gcctcgcttt gaacggccgg
     2521 cccatgcccg cggcattgcc gacgatcgcg acggtcaagc caccgcccga tgtcgtgatt
     2581 gtcgatgcgc ccgcgcagac cgatgccaag gagatcgacc tgcaggttcg catcaccgat
     2641 cggggcggcg gaatcggcac cgtgcgcacc ttggtcaacg gttcggcggt ctcccaggtc
     2701 ccgggtggcc cggctgccgg gagcgtccag tcgcgaagca ttcacgtgaa gctcgaacca
     2761 cacggcaacg acatccgtgt cgtcgccttc aatacggacg gatcggtgca gtcgaatgcc
     2821 gccgaggcca cggtcgcggc gaatttcgat ccttcgggca aaccgcagtt gcacgccctc
     2881 gtggtcggca ttcaggaatt cgacgtcgcg ggcaagaatc tcgagttctc ggtcgccgac
     2941 gcgaccgcaa tcgcgcaggt gctcgaaaag aaagcggctc cgcttttcag caaggtcaac
     3001 gttcagacac tcacgacgaa ggcgacgacg accaaggctg cgctgatgag tgcattcgaa
     3061 cgttatcggt cgatcgaccc tcaggatgtg ttcgtcttct acgcggcgac ccacggctcg
     3121 gtggagggtg atctggcgaa cccggaatac tacttgatca cctccaacgt caaagacacg
     3181 acgccagagg ccattcgccg cgacaccctg agcgggaccg aactacggca actcgtcgcc
     3241 agcattcccg ctacgcgcaa gctcctgctg cttgacacgt gccagtccgg tgccgcgggc
     3301 gacgcgatga tgctcaccac gcgaggcctc gaggaaaacg gggcggtcac ggtcctgtcc
     3361 ggcgcggtcg gcagtacggt gctttccgcg tcgacgcgcg agcagtcggc gctcgaggga
     3421 caggatggac atgggctttt cacgtgggtt ctcctgcatg ggcttcaggg ggacgccgat
     3481 gcgcgtcaca acgggtacat caagacattt gacctcgcgg cctacgtcga tgacgaagtt
     3541 ccgaagatat cccagcacaa acaaaatccc aacctgcaca atgcagggca atcgttcgag
     3601 atcgtgagca cccaatagcg ggacacagga gaatgcgatg acgtcttttc aaataccgcg
     3661 gacaagccgc ggcatcaaag gtgcaaacgg ccgcacggcc ttgacggtgc tcggcgcctt
     3721 gttgtcggcg tcaccggcgg gggcgcagac caccaacacg caacaaccga tcaagcggcc
     3781 ggtggtgccg gtgccgcaaa agcccgcgac gggcgtgcaa aacaatctgt ttagtgcgtt
     3841 cggcgcggcg aacaataatt cggctgcgca cactgcgccc gtgctatccc cccaggcgca
     3901 aaagtttctc aatccgaacg ccaacacggc tgcgggcaac aatacgcccg cgaacaggaa
     3961 tccgttggcc gcgctggtca atccgaacgg cgcggcgaac cgccaagcgc cgtccaacct
     4021 caacaacctg cagcggccgg tggcgaccgg cccgcaggtt ccagcgcgca gcgtccccgg
     4081 cttgccggct cctcgcggcg ccaaggagat gcaaggccac aacggcagca tcatccgcac
     4141 cgcctcggac gggtcggtcc tggat
//
LOCUS       DASWZT010000430         1855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_37253, whole genome shotgun sequence.
ACCESSION   DASWZT010000430 DASWZT010000000
VERSION     DASWZT010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37253"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VGE92_12260"
     CDS             complement(<1..406)
                     /locus_tag="VGE92_12260"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEY1314648.1"
                     /translation="MSASKDSFPVTTRFAPSPTGALHLGNARTALFSHLWARKSGGRF
                     ILRIEDTDAERSQLRFLDELMGEMRWLGLDWDEGPDIGGPSAPYSQAERGDLYRVLYA
                     RLEQDGQAYPCFCTPEELELSRKLQRMAGRPPR"
     gene            445..1065
                     /locus_tag="VGE92_12265"
     CDS             445..1065
                     /locus_tag="VGE92_12265"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY1314649.1"
                     /translation="MKQLLLVLGLISAAAPLVTFAAESPVAPAAAKTATPPAPAAQQV
                     QVFTSMGNFTLELNAERAPLTVAHFMRNVDSGQYTGTIFHRVIANFVIQGGGFDADYK
                     IKPAPTKVVNESGNGLTNQRGTVGMARGPEPHGSDCQFYVNLFDNEALDPNKTRWGYA
                     VFGKVVQGMDVVDKIGNVATGARGPFKEDAPLQPVVIQRIERVAGP"
     gene            1071..1793
                     /locus_tag="VGE92_12270"
     CDS             1071..1793
                     /locus_tag="VGE92_12270"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF003743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEY1314650.1"
                     /translation="MATLFISDLHIDASRPDITEQFLNFLSVEASSAEALYILGDLFE
                     SWIGDDAADPAQVKAIAGLKELTSSGVPCFVMHGNRDFLLAAEFCRSSGAQLLPDPLI
                     VTLYGEAVLVMHGDALCTDDRSYQRLRATVREADWQRQFLALSVASRRALAGAARAGS
                     QAHTAALEYAIADVNADSVAQALRAAGAATLLHGHTHRPAIHALQVDGRPCTRIVLGD
                     WYHQGSVLRWSRGGPDLQSLSR"
BASE COUNT          335 a          594 c          583 g          343 t
ORIGIN      
        1 agcgcggcgg gcggcccgcc atccgctgca gcttgcgcga caactccagc tcttccggcg
       61 tgcagaaaca cgggtacgct tggccgtctt gctctagccg tgcatacagc actcgataca
      121 gatcgccgcg ttccgcctgc gaataaggcg ccgacgggcc gccgatgtcg gggccctcat
      181 cccagtccaa gcccagccag cgcatttccc ccatcaactc gtcgaggaag cgcagttggc
      241 tgcgctccgc atcggtgtct tcgatgcgca agatgaagcg cccgccgctc tttcgcgccc
      301 aaaggtggct gaaaagggcg gttcgcgcgt tgccgaggtg taacgccccg gtggggctcg
      361 gcgcaaaccg ggtggtgaca gggaatgaat ctttgctcgc cgacatatct caagattgta
      421 cctattcgag tagaatctcg gagcatgaaa cagctattac tcgtcctcgg attgatttcg
      481 gcggccgctc ccttggtgac tttcgcggct gagagccccg tcgcgccggc ggcggccaag
      541 accgcgaccc cgcccgcgcc ggccgcgcag caagtccaag tattcacgtc aatgggaaat
      601 ttcacgctcg aattgaatgc cgagcgagcg ccgttgaccg tggcgcactt catgcgcaac
      661 gtcgattcgg ggcagtatac cggcacgatc tttcaccgcg tcatcgcgaa tttcgtaatc
      721 cagggcggcg gcttcgatgc cgactacaaa atcaaacccg cgcccaccaa ggtagtcaac
      781 gagtccggca atggcttgac caatcaacgc ggcaccgtcg gaatggctcg cggtccggag
      841 ccgcacggga gcgattgtca gttctacgtc aatttattcg acaacgaggc gctcgatccc
      901 aacaagaccc gttggggcta tgcggtcttc ggcaaggtcg tgcagggcat ggacgtggtc
      961 gataagatcg gcaacgtggc gaccggcgcg cgcgggccgt tcaaagaaga tgcgcccttg
     1021 cagccggtgg tgattcaacg aatcgagcgc gtcgccggcc cctaagcgaa gtggcgacgc
     1081 tcttcatctc cgatttgcac atcgacgcca gccggccgga catcaccgaa caatttctga
     1141 attttctctc cgtcgaagcc agcagcgccg aggctctgta tattctcggc gacttgttcg
     1201 agtcgtggat tggagatgat gccgccgatc cggcgcaggt caaggcgatc gccggtctaa
     1261 aggagttgac gtcgagcggc gtgccgtgct tcgtgatgca cggcaatcgc gactttttat
     1321 tggctgccga gttctgccgc tcgagcggcg cgcagcttct gcccgatccg ctgatcgtca
     1381 ccctgtacgg cgaagcggtg ctggtgatgc atggcgatgc gctgtgtacc gacgatcgat
     1441 cgtatcagcg gctgcgcgcc acggtgcgtg aggccgattg gcaacggcag tttctcgccc
     1501 tctccgtggc gtcgcgccgc gcgctcgcgg gtgcggcgcg cgccggcagt caggcgcaca
     1561 ccgccgcgct cgaatacgcg attgccgacg tcaatgccga cagcgtggcg caggcgttgc
     1621 gcgctgccgg cgccgcgacg ctgctgcacg gtcacaccca tcgcccggcg atccacgcgt
     1681 tgcaggtcga cggccgcccc tgcacgcgca tcgtactcgg cgattggtac catcaaggca
     1741 gcgtattgcg ttggagccgc ggcggaccgg atctgcagtc gctgtcgcga tgagagcccg
     1801 gagaatcacg aggttctaac ttgccgcggg cggccccgag tgaaaccgaa acacc
//
LOCUS       DASWZT010000431         3351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_61420, whole genome shotgun sequence.
ACCESSION   DASWZT010000431 DASWZT010000000
VERSION     DASWZT010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61420"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VGE92_12275"
     CDS             <1..1081
                     /locus_tag="VGE92_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY1314651.1"
                     /translation="SVAERISKRVPAAELVRFSNSGTEAVMAGLRLARAYTGKDDYVI
                     LEGGYHGLFDAAMWFTPMDKWSQVGDPEVKPYSEGVPLSTRSFAHFVQANDANQLEDV
                     FKRHSQRIACMLIEPIMGNCLGIAAEPEYLRAARALCDKHGVVLLIDEVKTGFRVARG
                     GVQELYGVKADLCTFAKAVANGYPISVLAGREEIMRKIGKGVAHGGTYTAHSISLAAA
                     EKTLQILDETDALERIADYGTRLRNGMRAVLNARGIAHSFVGHPSMSGLYFSHEPPRN
                     YRAWKGSDYSFYDAMAQVLHDEGVLCEPDSREPWFVSAAHDASCLADTLRAFEIAVDA
                     TLKSTAAPQQTARGAQAVATHKVSA"
     gene            1078..2664
                     /locus_tag="VGE92_12280"
     CDS             1078..2664
                     /locus_tag="VGE92_12280"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY1314652.1"
                     /translation="MNLDAIVVGAGHNGLVAACYLARSGLKVLVLERQPFIGGAAVSR
                     RLYEDFTYSNCSYVCSLLRPEIIRTLELPKYGLQVIPYEGGCTMMQGGGHLALYDNHD
                     ALRREISRHSKRDAEAYDRYSRDVMRQCKFIKPLLMREPPDPTSFKPRDLMELLYLGK
                     RFHGLGESRMYDTLRFWTMSAADFLDEYFESEIVKAHFAGSSIIGTALGPRSPGTAYV
                     LLHHYMGDIDDTVGAWGFARGGMGAISNALGASFQASGGTIRSAAPVAQILVRSGRAY
                     GVALQDGEEIHAPLVISNLDVRRTFLETMDAKDLPGEFLAQVKNFKIRGSSGKLNIAL
                     DGLPRFPAIPHGSPSTRGDMHVTDTIEMMERAYDDWKDGRWSRAPYIDMLIPSQIDPT
                     MVPDGKHYMSVFVQYCPYELADGPWDAAKRKAFGDTVIGTIAQHSPNFKELILHAEIR
                     TPWDIENEVGLTEGNIFQGELTMDQLLFNRPVPGYAQYRAPVRGMYMCGSSTHPGGGV
                     MGAPGANAARAVLRDLGRLH"
     gene            2568..>3351
                     /locus_tag="VGE92_12285"
     CDS             2568..>3351
                     /locus_tag="VGE92_12285"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1314653.1"
                     /translation="MRIQHASGRRCHGCAGCKRGAGRAARSRQAALMAAAPARFDCIV
                     IGGGHNGLVCAATLARAGRSVVLLEAADRVGGAALTREFAPGFRVSAGAHLLHLMPAD
                     LKRELKLAEHGLEWAAQGMPTTALVPGGSGLDLSPGSAAPGLSPADAKAYAEYIARMR
                     RFAAALVPALSRVPPRLGTSAWADRLSWLRLGWQIRKLGRRDMRELLRIGGMNVYDLL
                     EESFESAPLKGALGLDAVLGANFGPRSPGTVLTLLYRLAAEVA"
BASE COUNT          572 a         1035 c         1124 g          620 t
ORIGIN      
        1 cagcgtggct gagcgaatct ccaaaagggt gcccgccgca gaactcgtgc gtttttccaa
       61 ttcgggtacc gaagcggtga tggccggctt gcgcctggca cgggcctaca cgggaaagga
      121 cgattacgtg attctcgagg gcggctacca cgggctgttc gatgcggcga tgtggttcac
      181 gccgatggac aaatggtcac aggtgggtga tccagaggtc aagccgtaca gcgaaggggt
      241 gccgctgagc actcgcagct tcgcgcactt cgtgcaagcc aatgacgcca atcagttgga
      301 ggacgtgttc aagcgtcact cgcagcgcat cgcctgcatg ttgatcgagc ccatcatggg
      361 caactgtctc ggcatcgccg ccgagcccga atatctgcgc gcggcgcgcg cgttgtgcga
      421 caagcacggc gtcgtgctgc tcatcgatga agtgaagacc ggatttcgcg tggcgcgcgg
      481 cggcgtgcag gaactgtacg gggtcaaggc ggacctgtgc acctttgcca aggccgtggc
      541 gaacggttat cccatctcgg tgctcgcggg ccgcgaggag atcatgcgca agatcggcaa
      601 gggcgtcgcg cacggcggca cctatacggc gcactcgatc tcgctcgcgg ctgcggagaa
      661 gacgctgcag attctcgacg agaccgatgc tttggagcgc attgcggatt acgggacgcg
      721 gctgcgcaac ggcatgcgtg cggtactcaa tgcacgcggc attgcgcaca gcttcgtcgg
      781 ccatccgtcc atgagcggct tgtatttctc gcatgagccg ccgcgcaatt accgcgcgtg
      841 gaagggcagc gattactcct tctacgacgc catggcgcag gtgctgcatg atgagggcgt
      901 gctgtgcgag cccgactcgc gcgagccgtg gtttgtatcg gcggcacatg atgcgtcatg
      961 tctcgcagat acgctgcggg ctttcgagat tgcggtcgat gcgacgttga agtcgaccgc
     1021 ggcgccgcaa cagacggcgc gcggcgccca ggcggtcgct acgcataaag tgtctgcgtg
     1081 aatctcgatg ccatcgtcgt cggggccggc cataacggct tggtcgcggc gtgctatctc
     1141 gcgcgctccg gactcaaagt gctggtgctc gagcgccagc cattcatcgg cggcgcagcg
     1201 gtcagccgcc ggctgtatga ggattttacc tactcgaatt gttcgtacgt ctgcagtctc
     1261 ttgcggccgg agatcattcg taccctcgag ttgccgaagt acggcctgca ggtgattccc
     1321 tacgagggcg gatgcaccat gatgcagggc ggcggtcatc tcgcgctgta cgacaaccac
     1381 gacgcgctgc ggcgcgagat ctcgcggcac tcgaagcgcg atgcggaggc ctacgatcgt
     1441 tattcgcgcg acgtcatgcg ccagtgcaag ttcatcaagc cgctgctgat gcgcgaaccg
     1501 ccggacccga cctcgttcaa accgcgcgac ctgatggaac tgttgtacct cggcaagcgt
     1561 ttccacggcc tcggcgaatc gcgcatgtac gataccttgc gcttctggac catgagcgct
     1621 gccgattttc tcgacgagta tttcgagagc gagatcgtca aggcgcactt cgcgggcagt
     1681 tccataatcg gcacggcact cgggccgcgc tcacccggga ccgcctacgt gctgctgcat
     1741 cactacatgg gcgacatcga cgacacggtc ggcgcctggg gatttgcccg cggcggcatg
     1801 ggagcgatca gcaacgccct cggcgcttcg tttcaagcga gcggcggcac gatcaggagc
     1861 gccgcgccgg tcgcacaaat tctggtgcgc agcgggcgcg cctacggcgt tgcgttgcag
     1921 gacggcgagg agatccacgc gccgctggtg atctccaact tggacgtgcg ccgcacgttc
     1981 ctcgagacca tggatgccaa ggatttgccg ggcgaattct tggcccaagt caagaatttc
     2041 aagatccgcg gctcgtcagg gaaactgaac atcgcgttgg acggcctgcc gcgttttccg
     2101 gccattccgc acggctctcc gagtacccgc ggcgacatgc atgtcacgga tacgatcgaa
     2161 atgatggaac gcgcctatga cgattggaag gacggacgct ggtcgcgcgc gccgtacatc
     2221 gacatgctga tcccctcgca aatcgatccg accatggttc ccgacggcaa gcattacatg
     2281 tcggttttcg tgcagtactg cccttacgaa ttggcggacg gtccgtggga tgcggccaag
     2341 cgtaaagcct tcggcgatac cgtcatcggc acgatcgcgc agcacagtcc gaacttcaaa
     2401 gagctgatcc tgcacgccga aattcgcact ccctgggaca tagagaacga agtggggctg
     2461 accgagggca acatcttcca aggcgaattg accatggatc agctgctgtt caaccggccg
     2521 gtgccgggct atgcacagta ccgcgcgccg gtgcgcggca tgtatatgtg cggatccagc
     2581 acgcatccgg gcggcggtgt catgggtgcg ccgggtgcaa acgcggcgcg ggccgtgctg
     2641 cgcgatctcg gcaggctgca ttgatggccg ccgcccccgc gcgtttcgat tgcatcgtga
     2701 tcggcggcgg ccacaacggg ctggtgtgcg ccgccacact ggcgcgcgcc ggccgctcgg
     2761 tagtgctgct cgaggccgcc gatcgcgtcg gcggcgccgc gctgactcgc gagttcgccc
     2821 ccggatttcg cgtctcggcg ggcgcacatc tgctgcattt gatgccggcc gacctgaagc
     2881 gcgaattgaa gctcgccgag cacggactcg aatgggcggc gcagggcatg ccgacgacgg
     2941 cgctcgtgcc gggcggcagc ggccttgatt tgagccccgg gagcgccgca ccgggcctgt
     3001 cgccggcgga tgcaaaggcc tatgccgagt acatcgcgcg catgcgccgc tttgccgcgg
     3061 cgctggtgcc ggcgctgtcg cgcgtgccgc cgcgcctcgg tacgagcgcc tgggctgatc
     3121 ggctgtcgtg gctgcgtctt ggctggcaga ttcgcaagct cggccgtcgc gacatgcggg
     3181 agctgctgcg aatcggcggc atgaacgtct acgatttgct ggaagagagc ttcgaatcgg
     3241 cgccgctcaa gggtgcgctt ggactcgacg ccgtgctcgg cgcgaacttc ggtccgcgct
     3301 cgcccggcac ggtattgacg ctgctgtatc gtcttgcggc ggaggttgcg g
//
LOCUS       DASWZT010000432         5314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_80836, whole genome shotgun sequence.
ACCESSION   DASWZT010000432 DASWZT010000000
VERSION     DASWZT010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80836"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VGE92_12290"
     CDS             complement(<1..739)
                     /locus_tag="VGE92_12290"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEY1314654.1"
                     /translation="MSAIEDPDLERRWFPICNRDDLQAMHIVETDLLGRELAVWRGSG
                     GGVNVWANRCPHRGMRLTVGAIVAGELRCAYHGYRYAEGSGMCTSVPAQPGRVPPRSL
                     CVSTYPVFEAGNLVWTRLLSDESADAAPPLIAGDSTPLYGLAVKSRANDVAALLARYR
                     FRPSAALADPQAEDELCATRQIDAYSFHSVARKGSLTSEVRFFLQPVDAQCTVVHGIL
                     CGEIAAQLKIPALRHHTQQLTRLRDACE"
     gene            complement(736..1236)
                     /locus_tag="VGE92_12295"
     CDS             complement(736..1236)
                     /locus_tag="VGE92_12295"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEY1314655.1"
                     /translation="MDRLSDLAYLRAAIAVAAQAKAKGNHPFGAVLVDADGKVVAEGE
                     NTFLTDGGPGHAETNLARFAARTFPADYLEKCVLYTSVEPCCMCAGSIYWANIGAVVF
                     GMTEHRLLEITSGHSENLTQDLPCSVVFAAGQRKVEVRGPFAELADEIAGAHMGFWKS
                     RGSGTE"
     gene            complement(1250..3673)
                     /locus_tag="VGE92_12300"
     CDS             complement(1250..3673)
                     /locus_tag="VGE92_12300"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1314656.1"
                     /translation="MMAFLALSGACATRALADPAASQSVAANSDSLEEIQVVAQKQNI
                     DLQKAPVAITALAGDSLAQLNIVNPIDLNGQVPGLVITQSEGYNRSVSIRGIGFNVPQ
                     DDSAQTSVSYHEDGIYIAFPVALDSGFLDVDHVEVLRGPQGTVFGQNAVGGTINVISK
                     QPTFDGIDGFVDASGGSYHLEHLTAAINIPLSSTFAIRAAVDQITQKGYVKATEVPGD
                     PGFDLNNQNSWHARLQALWQTTDDLSVLLRAEYARANQHESSGKNINDPNPDPYQQTS
                     DWPGQMLYNQQLGAATITYNLSAATLKVLSSYQEVNHHGSVNEDGESLALITSQGSPH
                     DVEWFEHNSKSLTEEINLGSKPGGALDWIVGAFYLKSTTTVAYDQYNLFGGEPTLGGT
                     AAPNLLTVPESTVNDPNSALGQYIVNAIYAGTLYFQNFGVEHRDSASGYGQGIWHVTD
                     ALRLTAGLRYTHDHNTTWFDDYYDLFPPGHPIFYEQTSDKATWRVALDYDVTPVNLVY
                     GSVSTGFKPGGGNISNCPSGAPVGSGCPITAYPNGEPYQFKPETITAYEIGSKNSLFD
                     KKLNLNLATFLYQDENMQYQGDDLIPYQGGVGNIPKVRIVGLEAEMAALLPLNLRIDS
                     NATWEHGKITTHFEALDNYSGNLANNAFLALPGITYDDFLDAYSNPTGANGQLLQTFR
                     KKAYFDVYGNAPPSLPSLIATINLSHTLSIGSESSLLSRLSAQYRSDYATTIFGKTPI
                     YTAPSYTMFNLFFDYVLAAHSWDFSLAVNNVFDRAEVSSRFTNQFGAETTQVYAPPRE
                     FVAGAHYKF"
     gene            4066..5055
                     /locus_tag="VGE92_12305"
     CDS             4066..5055
                     /locus_tag="VGE92_12305"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF004771.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine dehydratase"
                     /protein_id="HEY1314657.1"
                     /translation="MSSKGLALEMSELENAAALVRKIVPPTPQYAWPKLSRRAGCTVW
                     VKHENHTPTGAFKVRGGLVYLDRLRQTEPNTRGVITATRGNHGQSIAFAAARAAMPAT
                     IYVPHNNSADQNSAITAFGARVVEYGKDFDDAKREAHRVAAAAELHFVPSFHRHLVAG
                     VATYALELLAAVAGLDAVYVGVGMGSGISGLIAVRDLLGLRTEIVGVGAANAPATALS
                     YAARCAVNASSARTFADGLATREPSEEAIAIICRGASRIVEVGEDAIAEAMRIYFDDT
                     HQVVEGAGAAPLAALLLERQRMANKNVALVLSGGNIERARFLQVLGGTTPSAA"
     gene            complement(5077..>5314)
                     /locus_tag="VGE92_12310"
     CDS             complement(5077..>5314)
                     /locus_tag="VGE92_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314658.1"
                     /translation="IDMALSVMADIAGADYAQMVQLALEYAPEPPFDCGRPERARPAI
                     VAAARKRLDSLGAQRAAAIERALANLKAEVARSA"
BASE COUNT         1000 a         1589 c         1668 g         1057 t
ORIGIN      
        1 gttcgcaggc gtctcgcaag cgcgtgagct gctgagtatg gtgccggagc gccggaatct
       61 tgagctgcgc ggcaatttct ccacacaaaa taccgtgcac gaccgtgcac tgtgcgtcga
      121 ccggttgcag gaagaaacgc acttcgctcg tgagggaacc cttgcgggcc accgaatgga
      181 agctgtatgc atcgatctgc cgggtcgcac acagctcgtc ttcggcttgc ggatctgcga
      241 gcgcagccga tggcctgaag cgatatcgcg cgagaagtgc tgcaacgtcg ttcgcccggg
      301 atttgaccgc gagcccatac agcggcgtgg agtctcccgc aatcaacggc ggcgcagcgt
      361 cggccgattc atcgcttagc agccgggtcc agaccagatt tccggcctcg aacaccgggt
      421 aggtgctgac acataaggat cggggtggaa ctcttccggg ttgcgcagga accgaggtac
      481 acatccccga tccctcagcg tatcgatagc cgtgataggc gcaacgaagt tctccagcca
      541 cgatggcgcc gacggtgaga cgcatgccgc gatggggaca gcggtttgcc cacacgttca
      601 cgccaccgcc gctgccccgc cagactgcga gttcgcgtcc caagaggtcg gtctcgacga
      661 tatgcatcgc ctgcaagtcg tcgcggttgc agatggggaa ccaacggcgc tcaagatcgg
      721 gatcctcgat ggcgctcact cggtgccgct gcctcggctt ttccaaaatc ccatatgcgc
      781 ccccgctatc tcgtcggcca attcggcgaa tgggccgcga acctcgactt tgcgttgacc
      841 cgccgcgaat accacgctgc acggcaggtc ttgggtcaga ttttcgctgt ggccgctggt
      901 gatctcgagc aagcgatgct cggtcatgcc aaacaccact gcgccgatgt tcgcccaata
      961 aatactgccc gcgcacatgc agcacggttc gaccgaagta tacagaacgc atttctcgag
     1021 ataatcggcg ggaaaagttc gcgcggcgaa gcgcgccaga ttggtttccg cgtgaccggg
     1081 tccgccgtcc gtcaggaagg tgttttcgcc ctccgccacg accttgccat ccgcgtcaac
     1141 cagcaccgcg ccgaacgggt gattgccctt ggccttggcc tgcgcggcga ccgcgatggc
     1201 cgcgcgcaga taggcgaggt cggaaagtcg atccacgggg gttcgctcct tagaatttgt
     1261 aatgcgcccc cgccacgaac tcgcgcggtg gtgcgtatac ctgggtggtt tccgcgccga
     1321 actgattggt gaagcgcgac gacacttcgg cccggtcaaa cacgttgttc accgccagcg
     1381 aaaagtccca actatgcgcc gccaagacgt agtcgaagaa cagattgaac atcgtatacg
     1441 acggtgccgt atagatcggc gtcttgccga agatggtcgt ggcgtaatcg gagcgatact
     1501 gggcgctcag ccgcgataac aacgacgact cgctgcctat gctgagggtg tggctcaaat
     1561 tgatcgtcgc aatcagcgag ggcagactgg gcggcgcatt gccgtaaaca tcgaagtacg
     1621 ccttcttgcg aaacgtctgc agcagctggc cgttcgctcc ggttggattc gagtaggcgt
     1681 cgaggaaatc gtcgtaggtg atcccaggga gggcgaggaa ggcgttgttc gcgaggtttc
     1741 cggaataatt gtcgagcgcc tcgaagtgcg tggtgatctt gccatgctcc caggttgcgt
     1801 tgctgtcgat gcgtagattg aggggcagca gcgccgccat ctcggcttcc aggccgacaa
     1861 tgcgcacctt cggaatgttg ccgacgccgc cctggtaggg aatcaggtca tcgccctggt
     1921 attgcatgtt ttcgtcctgg tacagaaacg tcgccaggtt caggttcagc ttcttgtcga
     1981 acagggaatt cttcgagcca atctcatacg ccgtgatcgt ttccggcttg aactgatacg
     2041 gttcgccgtt cggatacgcc gtgatcgggc agccgctgcc gaccggtgcg cccgatgggc
     2101 agttcgagat gttgccgccg ccgggcttga agccggttga cacgctgccg tagaccagat
     2161 tcacgggggt cacatcgtag tcgagcgcca cgcgccaggt ggctttatcc gaggtctgtt
     2221 cgtagaagat cggatgtccc ggcggaaaca gatcatagta gtcgtcgaac cacgtcgtgt
     2281 tgtgatcgtg cgtatagcgc aggccggccg tgagccgcag agcatccgtg acatgccaga
     2341 ttccctggcc gtaaccggaa gccgagtcgc gatgctcgac gccgaaattc tgaaaataca
     2401 gcgtgccggc gtagatggca ttgacgatgt actgacccaa cgcgctgttg gggtcgttca
     2461 cggtcgactc gggcacggtc agcagattcg gcgccgccgt gccacccaag gtcggctcgc
     2521 cgccgaacag gttgtactga tcgtaagcga cggtggtggt gctcttgaga tagaacgcac
     2581 cgacgatcca atcgagcgca ccgcccggct tggaacccag gttgatttcc tcggtgaggc
     2641 tcttcgaatt gtgctcgaac cactccacat catggggtga accttgactg gtgatcagcg
     2701 ccagcgactc gccatcctcg ttgaccgacc cgtgatggtt gacctcctgg tagctcgaca
     2761 ggactttcaa ggtcgccgcc gacaagttgt aggtgatcgt agcggcgccg agttgctggt
     2821 tgtagagcat ctgccccggc cagtccgacg tctgttggta tggatccgga ttcggatcat
     2881 tgatgttctt tccggaggat tcatgctgat tggcgcgtgc gtattcggcg cgcagcagca
     2941 ccgacaagtc gtcggtcgtc tgccacagcg cctgcaggcg cgcgtgccag ctgttttggt
     3001 tgttcaagtc gaagcccggg tcgcccggca cctcggttgc cttgacgtag cccttctggg
     3061 tgatctgatc gaccgctgct cgaatggcga aggtgctcga gagcgggatg ttgattgccg
     3121 ccgtgaggtg ttcgaggtgg taggaaccgc cggaggcgtc gacgaagccg tcgatcccat
     3181 cgaaggtcgg ctgcttgctg atcacgttga tcgtgccgcc gaccgcattc tggccgaaca
     3241 ccgttccctg tgggccgcgc agcacttcga cgtgatcgac gtcgaggaat cccgagtcga
     3301 gcgcgaccgg aaaggcgatg taaatgccgt cctcatgata ggagaccgag gtttgcgccg
     3361 aatcgtcctg cggtacgttg aagccgattc cgcggatcga cacgctgcgg ttataacctt
     3421 cgctctgcgt gatcacgagt cccgggacct ggccgttcaa gtcgatggga ttgacgatat
     3481 tcagctgtgc gagcgagtcg cccgccaagg cggtgatggc gaccggcgct ttttgcaggt
     3541 cgatattctg cttctgcgcg acgacctgga tttcctccaa cgaatcgctg tttgccgcaa
     3601 cggattggct ggccgccggg tcggcgagcg cgcgcgtggc gcaagctccg ctcaaggcca
     3661 ggaatgccat catgataagc gctgaacaag gccgcatcga gttctccctt caaggattgc
     3721 aagtcgcaag gctcctttcg ctgcaagcaa agcgacgcac gcttcgcctg tcgaaaccgc
     3781 cgttgaaaca atccccaaat tgccgatttc ccggagggtc tatgccctct tccgcatgat
     3841 gggatcgatc ggcggtcgct cgaaatcctt aaaaaaactc cagcccgttc aacgagaacc
     3901 atcggaatat gtcattcgtg tgaaagcgtg tcaatgaata cgcccactga acaacactgc
     3961 ggcacaggcg ggcgcaatcg cggagtcgct gaaatgtgca gggtgccgta acgggcaggc
     4021 gcttataatt tgcgggagat actcggtccg tccgggggag atgatgtgag ttcgaaaggc
     4081 ctggcacttg agatgagcga attggaaaat gccgcggcgc tggtgcgcaa gatcgtgccg
     4141 cccactccgc aatacgcatg gccgaaactc agccgtcgtg ccggatgcac cgtctgggtg
     4201 aagcacgaga atcacacgcc caccggcgca ttcaaggttc gcggcggcct agtgtacttg
     4261 gatcggctcc gccaaacgga acccaacacg cgcggagtca ttacggcaac tcgcggcaat
     4321 catgggcagt cgattgcatt cgccgccgca cgtgccgcca tgccggcaac catctacgtg
     4381 ccgcacaaca actccgccga tcaaaactcc gcaatcaccg cattcggcgc ccgggtggtc
     4441 gagtacggaa aagatttcga tgacgcaaag cgcgaggcgc atcgcgtcgc ggccgccgcc
     4501 gaactgcatt tcgtcccgtc ctttcatcgc catctggtcg ccggggttgc gacctacgcc
     4561 ctggagctgt tggcggcggt cgctggcctg gatgccgttt atgtcggcgt cggcatgggg
     4621 tccggcatct cggggttgat tgccgtccgg gatctgttgg gcttgaggac tgaaattgtc
     4681 ggcgttggcg cagccaacgc gcctgccacc gcgctttcct atgcggcgcg ctgcgcggtc
     4741 aacgcttcgt cggcgcgaac ctttgccgat ggtctggcga cacgcgagcc cagtgaagag
     4801 gcgatcgcga tcatctgccg cggcgcttca cgcattgtcg aagtcggcga agacgccatc
     4861 gcggaggcca tgcgcattta tttcgatgat acgcaccagg tcgttgaagg cgcgggcgca
     4921 gcgccgctcg ccgccttgct tctggaacgc cagcgaatgg ccaacaagaa tgtggcgctg
     4981 gtattgagcg gcggcaacat cgaacgtgcg cgcttcttgc aggttcttgg cggcacaacg
     5041 ccgtcggcgg cgtagcggcg ctcggcagtg cccatcttaa gcggacctcg caacctccgc
     5101 tttcagattg gcgagcgcac gctctatcgc tgcggcgcgt tgcgcgccga gcgagtccag
     5161 ccgctttcga gcggccgcga cgatggcagg ccgcgctcgc tcgggacgcc cgcagtcgaa
     5221 aggcggttcg ggcgcgtatt ccaatgcgag ctgcaccatc tgggcatagt cggcgccggc
     5281 aatgtcggcc atgaccgaca gcgccatgtc gatc
//
LOCUS       DASWZT010000433         5846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_71155, whole genome shotgun sequence.
ACCESSION   DASWZT010000433 DASWZT010000000
VERSION     DASWZT010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71155"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1367)
                     /locus_tag="VGE92_12315"
     CDS             complement(<1..1367)
                     /locus_tag="VGE92_12315"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEY1314659.1"
                     /translation="MSCLWDSIAPAGAAIDGRFLGRGAHVDLKDLSSGSMFGSELERF
                     RGRSVVIATREQLPTALALVELDGIARRMVLCTPDLTPQQLAGVAATAEADAIVLDAP
                     PGAATPASLTPFVLAPRPLPATMQRSGGVQTEWILLTSGTTGTPKLVLHTLASLSGEL
                     PQAAPSHMIWSTFYDIRRYGGLQIYLRAVLSRSPLVLSSAAEPTPQFLARAAAAAVTH
                     ISGTPSHWRRALMSGDAGILKPQYVRLSGEVADQPLLDNLRAVYPNARIAHAFASTEA
                     GLAFDVNDGREGFPEAFIDNPAAGIDLKVVNHTLRIRSRRNAARYLGATANVLLSDDG
                     YVDTGDMVELIGGRYYFRGRTGGVINVGGLKVYPEEVEAVLNADPRVRMSLVRARRNP
                     ITGALVVADVVLANPNSNAEADAPERIKNDLLEACRRTLASHKIPAMLRFVPSLEFTA
                     AGKLVR"
     gene            complement(1480..2907)
                     /locus_tag="VGE92_12320"
     CDS             complement(1480..2907)
                     /locus_tag="VGE92_12320"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEY1314660.1"
                     /translation="MRLAAVSRISILGEALDGLGRWYVAVPPFLIIGFLATLFFLTGA
                     GQERLQEASERLQKSALREHAIDDLQLGLSRSVATQRGYLLTGDQRFLKAYNTSITEI
                     EPRLSRLQAAYAGSDSDLAEVRSLNVLIGKRLADLAMVVAIQKTQGASAAIALMKTSV
                     GLDAGEAIGESLEQLRQRESEERGAAEDHWFKSLALSRWVVLAGTLFNMLLVGVAARL
                     VYMDMRRRTLQTAELRDQKLRLEVEAEERNRELVELSTHLQNIAEREKAALARELHDE
                     LGGLLVGARMDISWAEQHIANLDPDVKMRLNRVQQSLAAGVDLKRRIIEELRPTLLDN
                     VGIFAALRWQMKETCGRAGVKCSESFPEQEPSFTSEAAIALFRIAQEAFMNIVKHSGA
                     MSMDIALDVDDDAIVMQISDDGKGIPGERQNAIGSHGLASMRHRVRALGGRLDLRSPP
                     SGGTSLLVRIPAANALQHAAEPAQL"
     gene            complement(3016..5340)
                     /locus_tag="VGE92_12325"
     CDS             complement(3016..5340)
                     /locus_tag="VGE92_12325"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY1314661.1"
                     /translation="MALRTRIALTFLVLLTAVLAAALTAVSRTNRGNAEVDAEHRLDA
                     GSAAFSQLLRSDRSVLRQAARAVALDYGFREAVAKSDTETLVSVLQNSGERIHAAMVV
                     LTSLDGKVIAASGSHLKPGTHFHLPPVRKDGDGEYATGVIVDDGRIYQLVAVQVRSPL
                     PVAWIAMGFELDAAKASDLAQITGLAVTLSVKSGGRWTDVVSTLPADEIHQTDVEIRR
                     IEMSNTDNVEAVAVLSRSLAAARAPFESLTRALFKIAAIGLLAFALAAFWLARNITRP
                     LQDLTQAVDQIRGGAYNVSLNVHRRDELGVLAEGLQLMQLAVQSRDQSIRRLAYEDSL
                     TGVMNRTAFGAALEQALSESGATVAVAVINLDRFRRINEHLGYSVGDAVLTKMAARLA
                     AVPSVNSAVARLAADQFAAFTRLKDTANLQAWGTSLVMALAEPVFVEAQPIDITATLG
                     LALAGDSTGSADELMRCADLALERARHEKRALAVYEPTFKPAARDQLSLLGELRSAIE
                     HDELRLFFQPKIELKTGRVSGAEVLLRWQHPTRGLLGPADFIPFAEQTGFIRRLTRWT
                     LDHAIAQGGAWQRAGTALPLAVNISADDIGDARLDARVASLLTRHQLPPALLTLELTE
                     SGFIEDPTRALRMLSAIAMLGVNLSIDDFGTGYSSLSHLARMPVNEVKIDRSFVQGLE
                     SDPEFAPVVRSAVDMGHALGLKVVAEGIETEASAARLRGLGCDIAQGYLYAKPMPLAV
                     LETWLEGKERVPVIAVPVDFKIDEVTDTVSLASY"
     gene            complement(5345..>5846)
                     /locus_tag="VGE92_12330"
     CDS             complement(5345..>5846)
                     /locus_tag="VGE92_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="methylamine utilization protein"
                     /protein_id="HEY1314662.1"
                     /translation="IVFDPLDATPAPSRDTASIDQVNKRFVPRVSVVRTGTTVTFPNS
                     DHIRHQVYSFSEPKQINLKLYAGSPKTPIVFDRPGLIVLGCNIHDSMVGFVGVVDSPY
                     FGKTSETGTVALNLPAGRYRLRVWNPNGLAALPAREITVGAAPLSIPLTIDLDNASAN
                     VAAWLE"
BASE COUNT         1076 a         1891 c         1861 g         1018 t
ORIGIN      
        1 cgcaccaatt tgccggccgc agtgaattcc agagaaggca cgaaacgcag catcgccgga
       61 atcttgtggc tcgccagtgt gcgccggcat gcctcgagaa gatcgttctt gattcgttcc
      121 ggagcgtccg cttcggcgtt gctattggga ttggccaaga cgacatcggc aaccacgagt
      181 gcgccggtga tcgggttacg gcgcgcccgg accagcgaca tgcgcacccg cgggtcggcg
      241 ttcagcaccg cttcgacctc ctccggatac accttgagtc cgccgacatt gatgaccccg
      301 ccggtgcggc ccctgaagta atagcgcccc ccgatcagtt ccaccatatc gccggtgtcg
      361 acgtagccgt catcgctcag cagcacattc gctgtggcgc cgagatagcg ggcggcgttg
      421 cggcgcgagc gaattcgcaa cgtgtgattc acgaccttaa gatcgatgcc tgcggcgggg
      481 ttgtcgataa acgcctcggg aaatccctcg cgcccatcgt tgacgtcaaa cgcgagtccc
      541 gcctcggtcg aagcaaaggc atgcgcaatg cgggcattcg gatacaccgc gcgcagattg
      601 tcgagcaacg gctggtccgc gacctcgccc gataatcgca catactgggg ctttagaatc
      661 ccggcatcgc cgctcatcaa tgcgcgccgc caatgcgaag gggtgcccga aatatgggtg
      721 accgcggcgg ccgcggcccg cgccagaaat tgcggtgtcg gttcagcggc gctcgacagc
      781 accaagggcg agcgcgacag cacggcgcgc aggtaaatct gcagcccgcc gtagcggcgg
      841 atgtcataaa acgtgctcca aatcatgtgg ctcggcgcgg cctgcggcag ctcgccgctc
      901 aaactcgcga gcgtgtgcag cacgagcttc ggcgtcccgg tggtccccga ggtcaacaga
      961 atccactcgg tttgcacgcc gcccgagcgt tgcatcgttg ccggcagcgg ccggggtgcg
     1021 agcacgaacg gcgtcaaaga tgcgggcgtc gccgcgccgg gcggggcgtc caatacgatg
     1081 gcgtcggcct cggccgtggc tgcaacgcct gcaagctgct gcggcgtgag atccggcgtg
     1141 cacagaacca ttcggcgcgc gatgccgtcg agctccacca gcgcaagcgc cgtgggcagt
     1201 tgctcgcggg tggcaatcac caccgagcgg ccgcgaaagc gttcgagctc ggacccgaac
     1261 atcgatccgc tgctcaggtc cttgagatcg acgtgcgcgc cgcggcccag aaacctaccg
     1321 tcgatcgcgg cacctgccgg cgctatggaa tcccaaagac aactcactgc atcgcactcc
     1381 gtttggcggc ccgggcaaat tctagggcaa tgtccgctcg agggacagct gacataattg
     1441 acggtcgcgg tgttcggcgc cgtgacgttt tcggcaattc tagagttgcg cgggctccgc
     1501 agcgtgctgc agggcgttgg cagcgggaat ccgcaccagc agactggtgc cgccggaagg
     1561 cggactgcgc agatccaggc gaccgcccaa ggcgcgcacc cggtggcgca tggacgccaa
     1621 gccgtgcgaa ccgatggcat tctgccgctc ccccggaatc cccttaccgt catccgagat
     1681 ttgcatgacg atggcatcgt cgtcgacgtc gagcgcgatg tccatggaca tcgcccccga
     1741 gtgcttcacg atattcatga acgcttcctg agcgatgcgg aacaaggcga tcgccgcttc
     1801 ggaagtaaac gacggctctt gctcggggaa tgactcgctg cacttgacgc cggcgcggcc
     1861 gcaagtctct ttcatctgcc aacgcagcgc ggcgaatatc cccacgttgt ccaacagcgt
     1921 gggccgcagt tcttcgatga tgcggcgctt caaatcgacg ccggcggcaa ggctttgctg
     1981 tacgcggttg agccgcatct tcacgtcggg atcgagattc gcgatatgct gttccgccca
     2041 agaaatatcc atgcgggcgc cgaccagcaa gccgccgagt tcatcgtgca attcgcgcgc
     2101 cagcgccgcc ttctcgcgct cggcgatgtt ttgcagatgg gttgagagct cgaccagttc
     2161 gcgattgcgt tcctccgctt cgacttcgag gcgtagtttt tgatcgcgca gttcggcggt
     2221 ctgcagcgtg cggcgacgca tatccatgta gaccaagcgc gcggcaacgc ccaccaacag
     2281 catgttgaat agcgttccgg cgagcaccac ccagcggctc aatgcgagcg atttgaacca
     2341 atgatcctct gcggcgccgc gttcttcgga ctcgcgctgg cgcaattgct cgagggattc
     2401 gccgatggct tcaccggcat ccaaaccgac gctggttttc atcagagcaa tcgcggccga
     2461 ggcgccttgg gtcttctgaa tcgcgaccac catcgccaga tccgccagcc gcttgccgat
     2521 cagcacgttg agcgagcgca cctccgccaa atcggagtcc gagcccgcat atgccgcctg
     2581 cagccgcgac agccgcggct cgatctcggt gatgctcgtg ttgtaggcct tgagaaatct
     2641 ctggtcgccg gtcagcaggt aaccgcgctg cgtggcgacc gatcgggaca ggccgagttg
     2701 cagatcatcg attgcgtgct cgcgcaaggc ggatttctgc agccgttcgc tcgcttcctg
     2761 caagcgctcc tgtcctgcgc cggtcagaaa gaacagcgta gccaggaacc cgatgatcag
     2821 gaacggcggc accgccacat accagcggcc caatccgtcg agcgcctcgc ccagaatgct
     2881 gatgcggctc actgcggcaa gacgcatggg tgtgcagcgg cgctatgccg atgactcagg
     2941 ggagggtcga cacgattcat tgcagaaata ctgtacgcga atttgcccga aacgcgagtg
     3001 ccgcgggaag cggcctcaat agctcgccaa gctcaccgta tcggtcacct cgtcgatctt
     3061 gaagtcgacc ggcaccgcga tcaccgggac gcgctccttg ccctcgagcc aggtctcgag
     3121 caccgcgagc ggcatgggct tggcatacag gtaaccctgc gcaatatcgc agccgaggcc
     3181 gcgcaatcgc gccgccgagg cttcggtctc gataccctcg gccaccacct tgagtccaag
     3241 agcgtggccc atgtcgaccg ccgatcgcac cacgggtgcg aattccgggt cggactcgag
     3301 gccctgcacg aaactacggt cgatcttgac ttcgttgacg ggcatgcggg caaggtggct
     3361 tagcgaggaa tagcccgtgc cgaagtcgtc gatggacagg ttcaccccca acatcgcgat
     3421 ggcgctcagc atgcgcagcg cccgcgtggg atcctcgata aatccgctct cggtgagttc
     3481 cagggtgagc agcgccggcg gaagttgatg ccgggtaagc aggcttgcca cgcgggcgtc
     3541 gagccgcgcg tcgccgatat cgtcggcgct gatgttcacc gcgagcggca aggccgtccc
     3601 ggcccgctgc caggcgccgc cctgcgcgat cgcgtgatcg agcgtccaac gcgtcaggcg
     3661 gcggatgaat ccggtctgtt cggcaaacgg aatgaagtcc gccggaccga gcagtcctcg
     3721 agtcgggtgc tgccagcgaa gcaggacttc ggcgccgctc acgcggccgg tcttgagttc
     3781 gatcttcggc tggaagaaca agcgcagctc atcgtgctcg atcgcgctgc gcagctcgcc
     3841 caacagcgac agctggtcgc gagccgccgg tttgaaggtt ggctcgtaca cggcgagcgc
     3901 gcgcttctcg tgccgtgcgc gctcgagcgc cagatcggcg cagcgcatca gctcgtcggc
     3961 cgagcccgtg ctgtcgccgg cgagcgccag gccgagagtc gcggtgatgt cgatgggctg
     4021 cgcctcgacg aatacgggct cggccagcgc catcaccagc gaagtgcccc aggcctgcag
     4081 attcgcggta tccttgagcc gcgtgaacgc cgcgaattga tcggcggcca aacgcgccac
     4141 cgccgaattc acagacggca ccgccgcgag acgcgccgcc atcttggtca gcacggcatc
     4201 gcccaccgaa tagccgagat gctcgttgat gcgcctgaaa cgatccaaat tgatcaccgc
     4261 aacggcgacc gtcgctccgg attccgacaa tgcttgttcc agggccgcgc cgaacgcggt
     4321 gcgattcatc acgccggtga gcgagtcttc gtatgccagg cgccggatgg attgatcgcg
     4381 ggactgcact gccaattgca tgagctgcag gccttcggca agcactccca attcgtcgcg
     4441 ccggtgcacg ttcaaagaca cgttgtaagc gccgccgcga atctgatcga ccgcctgtgt
     4501 gaggtcctgc agcggccggg tgatattgcg cgccagccag aaggccgcga gtgcgaaagc
     4561 gagcagtccg atcgccgcaa tcttgaacaa cgcccgagtc aggctctcga agggcgcgcg
     4621 cgccgcggcg agcgagcgcg acagaacggc gacggcctcg acgttgtccg tgttggacat
     4681 ctcgatgcgc ctgatttcga cgtcggtctg atggatttca tcggccggca gcgtactcac
     4741 gacatcggtc caccgcccgc cggacttcac cgacagggtc acggcgagac cggtgatttg
     4801 cgccagatcg cttgccttgg cggcatcgag ttcgaacccc atcgcgatcc aagcgaccgg
     4861 cagcggactt ctcacctgca ccgccacgag ttgatagatg cggccgtcgt cgacgatgac
     4921 cccggtcgca tactcgccgt cgccatcttt gcgcaccggc ggcagatgaa aatgagtgcc
     4981 gggtttcaga tgcgatcccg aggcggcaat caccttgccg tcgagcgagg tcagcaccac
     5041 catcgcggcg tgtatgcgtt cgccgctgtt ttgcagcacg gagaccaacg tctccgtgtc
     5101 gctcttcgcc actgcttcgc gaaatccata gtcgagggcg acggcgcgcg ccgcctggcg
     5161 caatacgctg cgatcgctcc ggagcagctg tgagaatgcc gcgctgcccg catccaatcg
     5221 atgttctgca tcgacttcgg cattgccgcg attggtgcgc gagacggcgg tcagcgcggc
     5281 agccagcaca gcggtcagca aaacgaggaa ggtcaacgcg attcgggtgc gtagtgccat
     5341 gccgctactc gagccacgcc gcgacgttcg cggatgcatt gtccaggtca atcgtcaggg
     5401 gaatgctcag cggcgcagcg ccgacagtga tctcgcgcgc cggcagcgcc gcgagcccat
     5461 ttggattcca gacgcgcagc cggtagcgcc cggcgggcag attcaaagca accgtccccg
     5521 tctcgctggt cttaccaaag tagggggagt ccaccacgcc gacgaaaccc accatgctgt
     5581 cgtgaatgtt gcagcccaag acgatgagtc ccggccgatc gaaaacgatc ggggtcttgg
     5641 gcgagcccgc atacaatttg aggttgatct gcttgggctc ggaaaacgaa tagacctgat
     5701 ggcggatgtg gtcgctgttc gggaaagtga ctgttgtgcc ggtacggacc acgctgacgc
     5761 gcggcacgaa gcgtttgttg acctggtcga tactggctgt gtcgcggctc ggggcgggag
     5821 ttgcatcgag cgggtcgaac acgatc
//
LOCUS       DASWZT010000434         2998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_17808, whole genome shotgun sequence.
ACCESSION   DASWZT010000434 DASWZT010000000
VERSION     DASWZT010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17808"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VGE92_12335"
     CDS             <1..181
                     /locus_tag="VGE92_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314663.1"
                     /translation="VGMVPGLTTLTRLDSRFRNGWRLRLRIPMMATARSGGWRPRIPI
                     DDDRWCGGVMAPLGR"
     gene            complement(434..1240)
                     /gene="istB"
                     /locus_tag="VGE92_12340"
     CDS             complement(434..1240)
                     /gene="istB"
                     /locus_tag="VGE92_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012090960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEY1314664.1"
                     /translation="MLTHPTLEQLRALKLDGMARAFTELEAQDRANELSHAEWLALLL
                     DREAASRDTRRFQTRMRAARLRHGQAAIEDVDYRTPRRLDKALFQQLASGRWFAEHRN
                     LLVTGPCGVGKSWLSCALAQKACRDGYSVHYARIPRLFAELELAHGDGRFARLFGTLV
                     KADLLILDDWGPDRLTASQRRDLMEIVEDRYGRGSTLITSQLPVDKWHEVIAEPTFAD
                     AILDRIVHNAYRLDLDGPSMRKIKAAEIEVAAPDPATDTKAAKPTKGARK"
     gene            complement(1251..2765)
                     /gene="istA"
                     /locus_tag="VGE92_12345"
     CDS             complement(1251..2765)
                     /gene="istA"
                     /locus_tag="VGE92_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011427313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEY1314665.1"
                     /translation="MRKIRDILRLKAGGLSKRRIAASLGLSATAAGDCIRRARVAGIG
                     WPVADDMTDEALERRLYPPPAATGKARRSQPDWARVHRELKRPGVTLQLLWEEHLTAH
                     CDGHGYSRFCELYQDWKGRLSPTMRQTHVAGERLFVDYAGTTLQVIDGATGEVHTAQL
                     FVAALGASSLTYAEATLTQNLADWIGSHTRAFAFYGGVTAMVVCDNLRSGITKASFHE
                     PAVNRSYAEMAAHYDTAIVPARPRKPRDKAKVEAAVLLATRWIIAKLRNRQFFSLAEL
                     NAAIRDCVAALNGRWSRHLGASRTQLFEQLERAALKRLPVEPYVYAEWKQCRAGLDYH
                     VEIDKHYYSVPHALLREELWARITARTIEVFHRGQRIAAHVRSSSNRAHTTLPEHMPS
                     SHRRYADWTPERLTRQAGEIGRNTAALVEIILREKRHPEQGFRACIGIVGLARTHGRE
                     RLEAACERALEIGARSYTSVVSILKNNLDRKQSAPATDGPAIAHTNIRGPGYFH"
BASE COUNT          468 a          964 c         1019 g          547 t
ORIGIN      
        1 ggtcggaatg gtaccaggac tgaccacgtt gacgcggctg gattcgagat ttcgcaacgg
       61 atggcgattg cgcctgcgca ttccgatgat ggcgaccgct cgttccggcg gatggcgacc
      121 acgtattccg atcgatgacg accggtggtg tggcggcgtg atggcgccgt tgggtcgata
      181 ggctgatgtc agcgttccgg atggccgtca agcatgttgg tgtgcttcgt tgtcggcgag
      241 agggacagcc tgatcgcgcg acaatctgga tgagacgagg cggccgggcg tatgggatcg
      301 cagtggcagc ccgtccccgt agtcaacggg agggacccgc gcgtgccgcg gcgtggcgtc
      361 agatccgacg cggcggcgcg cgcgggaggg gcctgttgat ccacgggggc gggcgggatg
      421 cggcatgggg cgttcatttg cgagctccct tggttggctt ggcggccttg gtgtccgtcg
      481 ccgggtccgg cgcggcgacc tcgatctcag ccgccttgat cttgcgcatc gaggggccgt
      541 cgagatcgag gcggtaggcg ttgtggacga tgcgatcgag gatggcgtca gcaaaggtgg
      601 gctcggcgat cacctcgtgc cacttgtcga ccggcaactg gctggtgatg agggtggagc
      661 cgcggccata gcggtcctcg acgatctcca tcagatcgcg ccgctggctg gcggtgagcc
      721 ggtccggtcc ccagtcatcg aggatcaata gatcggcttt gaccagcgtg ccgaacagcc
      781 gcgcgaagcg gccatcgcca tgggccagct cgagctcggc gaacaggcgc gggatgcgcg
      841 catagtgcac gctgtagccg tcgcgacagg ccttctgggc cagcgcgcac gacaaccacg
      901 acttgccgac cccgcacgga ccggtgacga gcaggttgcg gtgctcggcg aaccagcggc
      961 cgctggccag ttgctgaaac agtgccttgt cgagtcggcg cggggtacga tagtcgacat
     1021 cctcgatcgc ggcttggccg tggcgcaacc gggcggcacg catccgggtt tggaaccgcc
     1081 tggtgtcgcg gctcgccgcc tcgcggtcga gcagcagggc cagccattcg gcgtggctca
     1141 gctcgttcgc ccggtcttgg gcctcgagtt cggtgaaggc gcgcgccatg ccgtcgagct
     1201 tgagcgcacg cagctgttcg agggttgggt gtgtcagcat ttgtctctcc tcagtggaag
     1261 tagccagggc cacggatgtt ggtgtgggcg atcgcaggtc cgtccgtggc gggcgcggac
     1321 tgctttcgat cgaggttgtt cttcaggatc gacaccaccg aggtgtacga gcgcgcgccg
     1381 atctccagtg ctcgctcaca ggcagcctcg aggcgttcgc ggccgtgcgt cctggcgaga
     1441 ccgacgatgc cgatgcaggc gcgaaaaccc tgctccggat gtctcttctc gcgcaggatg
     1501 atctcgacca gcgccgcggt gttgcgcccg atctcgccgg cctgccgcgt gagccgttcc
     1561 ggcgtccaat cggcgtagcg ccggtggctc gacggcatgt gctcgggcag cgtggtgtgc
     1621 gcccggttcg acgaggaacg aacgtgcgcg gcaatccgct ggccacgatg aaacacctcg
     1681 atggtgcgcg ccgtgatgcg cgcccacagc tcctcgcgca gcagcgcgtg cggcaccgag
     1741 tagtagtgct tgtcgatctc gacatgatag tcgagcccgg cacggcactg cttccattcg
     1801 gcgtaaacgt aaggctcgac cggcagccgc ttgagcgccg ctcgttccag ctgctcgaac
     1861 agttgcgtgc ggctcgcgcc cagatgccgt gaccagcgtc cgttcagcgc cgccacgcag
     1921 tcgcggatcg ccgcgttcaa ttcggccagc gagaagaact ggcgattcct cagcttggcg
     1981 atgatccatc gcgtcgccag tagcaccgcc gcttccacct tcgccttgtc gcgcggcttg
     2041 cgtggccgcg ccggcacgat ggcggtgtcg tagtgggcgg ccatctccgc gtaactgcgg
     2101 ttgaccgctg gctcgtggaa gctcgccttg gtaatcccgg acctcaggtt gtcgcagacc
     2161 accatcgcgg tcacgccgcc gtagaatgca aaggcgcgcg tgtgcgagcc gatccagtcg
     2221 gcaaggttct gtgtcaaggt cgcttccgca taggttaagc tcgacgctcc gagcgctgcc
     2281 acaaacagct gcgcggtgtg tacctcgccg gtcgcgccgt cgatcacctg cagggtcgta
     2341 ccggcgtagt cgacaaacag ccgctcgccc gccacatgcg tctgtcgcat cgttggcgac
     2401 agccggccct tccagtcctg atacaactcg cagaaccgac tgtagccatg tccatcgcag
     2461 tgcgcggtga gatgctcttc ccacaacagc tgcagcgtca cgccgggccg cttgagttcg
     2521 cgatgcaccc gcgcccagtc cggctgcgac cgccgagcct tgcccgtcgc cgcaggcggc
     2581 ggatagaggc gccgctccag cgcctcgtcg gtcatatcgt ctgcaaccgg ccacccgatg
     2641 cctgccacgc gggctcgtcg gatgcagtct ccggccgctg tcgcgctcag cccaaggctg
     2701 gcagcgatcc tgcgcttgga caagccgccg gccttgagcc gcaaaatatc gcgaatcttc
     2761 cgcatcggta gtctctcctg aggcattccc cgtcctttcg aaaaaggagc aggcaagccg
     2821 attcgagccc tacccaaccg cgcctttcac gaccgtccac agccggtcgt catccgtcgg
     2881 aatgactggt cgtcatcgat cggaatacgt ggtcgccatc agtcggaacg agtggtcgtc
     2941 ttcaatcgga aaaggtggtc gccatcaccc ggtgcgcgca gccatgacgg aacttcag
//
LOCUS       DASWZT010000435         3687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_37293, whole genome shotgun sequence.
ACCESSION   DASWZT010000435 DASWZT010000000
VERSION     DASWZT010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37293"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="VGE92_12350"
     CDS             <1..1213
                     /locus_tag="VGE92_12350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="geranylgeranyl diphosphate reductase"
                     /protein_id="HEY1314666.1"
                     /translation="SNAAPLARAVSSAETFDVVVIGGGPSGATAATDLARQGHRVCLL
                     DRAGRIKPCGGAIPPKLMEEFDIPESLLVARVNSARMISPAQACVDMPIEGGFVGMVD
                     RETFDEWLRQRAACAGAQLRRARFERITRDGDELALVHFWESGQAQQVRARAVIGADG
                     AMSTVAKQCIPGAHRIRYVAAYHEIVRVPAAQGDEYSASRCDVYYQGKLSPDFYAWIF
                     PHGATASVGVGSAAKGFSLRGAVLELRALAKLAGVETLRREGAPIPMKPLRRWDNGRD
                     VVLAGDAAGVVAPASGEGIYYAMAGGRFAALAAAEYLNSGDARALRNARRRFMRAHGT
                     VFWVLGIMQRFWYSTDKRRERFVSICKDPDVQRLTWQAYMHKKLVRAKPLTHMRIFAK
                     DMAHLLGLARA"
     gene            1210..1731
                     /locus_tag="VGE92_12355"
     CDS             1210..1731
                     /locus_tag="VGE92_12355"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HEY1314667.1"
                     /translation="MTHAVGTRTGDLRRRLKPVAAAAVAASAVAALGALSTQLGSWYY
                     RLRKPSWQPADWLFGPAWTLIFALAALAAVLYWRAEDNRDRRLLVLGVFALNGFLNTL
                     WSLLFFRLQRPDWSLYEVGFLWASIVLLIVLLIRASRPAAWLLAPYLAWVSFAAILNW
                     KIVQLNAPFASGG"
     gene            1724..1999
                     /locus_tag="VGE92_12360"
     CDS             1724..1999
                     /locus_tag="VGE92_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314668.1"
                     /translation="MAELFATGRVIDGILGLMLMEFIGLVLLGRQSRRGLPASQLTFS
                     LGAGAALLLALRAALLGASWPRIALWLLAALVAHLSDLRLRWTYRRT"
     gene            2053..2391
                     /locus_tag="VGE92_12365"
     CDS             2053..2391
                     /locus_tag="VGE92_12365"
                     /inference="COORDINATES: protein motif:HMM:NF013517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HEY1314669.1"
                     /translation="MQNKISRRALVKSGLLAGALTPVSSLLAYRAYAAPLPPLDPNDP
                     TAKALGFVTDSSKVDVSTNPTHTAAQKCGTCAQFQGKTGDASGGCNIFTGHSVPQGGW
                     CKVWAQKPSA"
     gene            complement(2437..3018)
                     /locus_tag="VGE92_12370"
     CDS             complement(2437..3018)
                     /locus_tag="VGE92_12370"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY1314670.1"
                     /translation="MNRIVSACIAALLGTSAFAAPVTYILDPAHTFPSFAADHKGGLS
                     VWRGKFDATSGKVVYDKAAKSGSIDVTVDMSSIDFGMPKLNEHAKSAEIFDTAKYPTA
                     TYSGKFTKFSGDAPTEAQGTLTMHGVTKPVTLIINSFLCKNDPMSKKDVCGADASATF
                     NRADFGVNFGEKYGFKQDVSLQIQVEGSPAPAG"
     gene            3091..3597
                     /locus_tag="VGE92_12375"
     CDS             3091..3597
                     /locus_tag="VGE92_12375"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEY1314671.1"
                     /translation="MELFDAITTRSSAGLLAAPGPSPVELERLLQAAARAPDHGRLRP
                     WRFIVLEGAARESFAAAAAAAKRRLLPAMTAEQMAGERDKMLRSPTLVVAACVVDLAN
                     TKIPEVEQILAVGAAVENLFLAAHALGFGVMWKTGAAAYDAGVKALLGLQPHDHIAAI
                     LHLGTRLK"
BASE COUNT          615 a         1142 c         1208 g          722 t
ORIGIN      
        1 ctcgaacgcc gcgccgctgg cgcgggcagt cagctcggcg gaaaccttcg atgtggtggt
       61 catcggcggc ggaccgtcgg gagcgacggc cgcgacggat ttggcccgtc aaggtcatcg
      121 cgtctgcctc ctcgaccgag cgggccgaat caaaccttgc ggtggagcca taccgcccaa
      181 gctcatggaa gaattcgaca tccccgagtc gctgttggtg gcccgcgtca actcggcgcg
      241 catgatctcg ccggcgcagg cctgcgtcga tatgcccatc gagggtggat ttgtcggaat
      301 ggtcgaccgc gagaccttcg atgagtggct gcggcagcgg gccgcatgcg ccggcgcgca
      361 gctgcgacgc gcccggtttg agcgcattac tcgagacggc gacgagcttg cgctggtgca
      421 tttttgggaa tccgggcagg cacagcaagt tcgtgcgcgc gcggtgatag gcgcggatgg
      481 ggccatgtcg accgtcgcca agcaatgcat tcccggcgcg catcgcatcc gctacgtcgc
      541 cgcctaccac gaaatcgtcc gggtgccggc tgcgcagggc gatgagtact ctgccagccg
      601 ctgcgatgtg tactaccagg ggaaattgtc gcccgacttc tatgcgtgga tatttccgca
      661 tggcgcgacc gcgagcgtcg gcgtcggcag cgccgcaaag ggtttctcgc tgcgcggcgc
      721 ggtgctcgag ctgcgcgcac tcgcgaagct tgccggtgtc gagacgctgc ggcgtgaagg
      781 agctccgatc ccgatgaagc cattgcgccg ctgggacaat ggccgcgacg tcgtgttggc
      841 cggcgacgcg gccggtgtag tggctcccgc ctcgggcgag ggaatttatt atgcgatggc
      901 gggcgggcga ttcgcagcgc tcgccgccgc ggaatatctc aacagcggcg atgctcgtgc
      961 gctgcgcaat gcgcgccgcc gcttcatgag agcgcacggc acggtgtttt gggtgttggg
     1021 catcatgcag cgtttctggt attcgaccga caagcgccgc gaacgcttcg tcagcatctg
     1081 caaggatccc gatgtgcagc gcctcacctg gcaggcttac atgcacaaga aattggtgcg
     1141 cgccaaaccg ctgacacata tgcgaatctt tgccaaagac atggcgcatt tgctgggact
     1201 cgcgcgcgca tgacgcatgc cgtcggcacg cgcacgggcg atcttcgacg gcgcctcaag
     1261 ccggtggcgg cggcggcggt cgcggcgagt gcggttgccg cccttggtgc tctcagcacg
     1321 cagttgggct cctggtacta tcgattgcgc aaacccagtt ggcagccggc ggactggctg
     1381 tttggtccgg cgtggacctt gatattcgcc ttggcggcac tcgccgcggt gttgtactgg
     1441 agagcggaag ataaccgcga ccgccggctg ttggtgctcg gcgtattcgc gctcaacgga
     1501 ttcttgaata ccctgtggag cctgctgttc tttcggctgc agcggccgga ttggtcattg
     1561 tacgaagtgg gatttctctg ggcgtccatc gtgctgttga tcgtgctgct gattcgtgcc
     1621 agccgccccg cggcctggct gctcgcgccg tatttggcct gggtcagctt tgcggcgatt
     1681 ttgaactgga aaatagtgca gttgaacgct ccttttgcca gcggtggctg agctgtttgc
     1741 caccggtcgc gtgatcgacg gcatcctcgg attgatgctg atggagttca tcggcttggt
     1801 gctgctcggc cggcaatcgc ggcgtgggct gccggcatcg caactcacgt tcagcctcgg
     1861 cgcgggcgct gccttgctgc tggcattgcg tgccgcactg ctcggcgcat cttggccgcg
     1921 aatcgcgctc tggctgctcg ccgccttggt ggcgcacttg agcgaccttc ggctgcgctg
     1981 gacctatcgt cgcacatgag ccgtgttctt cacccgattc agttagaact ccaccacaag
     2041 gaaaaagctc tcatgcaaaa caagatttcg cgtcgcgcgc tggtcaagag cgggctgctc
     2101 gccggcgccc tcactccggt ctcgagcctg ctggcttatc gcgcctatgc tgcgccgctg
     2161 ccgccactcg acccgaacga tcccaccgcc aaagcgctcg gctttgtcac cgactcgtcc
     2221 aaggtcgacg tgagtaccaa tccgacgcat accgcggcgc aaaaatgcgg tacgtgcgcg
     2281 cagtttcagg gcaaaacggg cgatgcaagc ggcggctgca atatttttac gggacacagc
     2341 gtgccgcagg gagggtggtg caaagtgtgg gcgcaaaaac caagcgccta ggtgtcggag
     2401 gaggtgagcg caagcccacc tcctacccga taaagctcaa ccggccgggg ctgggctgcc
     2461 ttcaacctgt atctgcagcg atacgtcctg cttgaagccg tacttctcgc cgaagttcac
     2521 gccgaagtcg gcgcgattga aggtcgcgga cgcatccgcg ccgcacacat ctttcttgct
     2581 catcgggtcg tttttgcaaa ggaatgagtt gattatgagc gttaccggtt tcgtcacgcc
     2641 gtgcatggtc agggtgcctt gcgcttcggt gggcgcatcg ccactgaact tggtgaactt
     2701 gccgctgtaa gttgcggtcg ggtatttcgc ggtgtcgaag atctcggccg acttggcatg
     2761 ttcattcaat ttcggcatgc cgaagtcgat cgagctcatg tccacggtaa cgtcgatgga
     2821 gccggacttt gccgccttgt cgtacaccac cttgccggag gtcgcatcga acttgccgcg
     2881 ccacaccgac aaaccaccct tgtggtcggc ggcgaaactc ggaaaggtgt gcgcaggatc
     2941 gaggatgtaa gtgaccggcg ccgcgaacgc gctcgtgccg agcagcgcgg cgatgcacgc
     3001 cgaaacaata cgattcattg aagactcctt aaaaaggccc tgtgggtata cttcgcagcc
     3061 gaaatcatag ccgataaccc ggagtctcca atggaattat ttgacgccat caccacgcga
     3121 tcctcggccg gactcttggc ggcacccggg ccgagcccgg tcgagctcga acggctgctg
     3181 caagcggcgg cccgcgcgcc ggatcacgga cgcctgcgcc cgtggcggtt catcgtcctc
     3241 gagggcgcgg ctcgagagtc gtttgccgcc gccgccgctg ccgccaaacg ccgcttgttg
     3301 ccggcgatga ccgcggagca gatggccggg gaacgggaca agatgctgcg ctcgccgacc
     3361 ttggtggtcg ccgcttgtgt ggtggatctc gcgaacacga aaattccgga ggtcgagcaa
     3421 atcctcgcgg tcggggccgc ggtggagaat ctgttcctgg cagcgcatgc gctgggtttc
     3481 ggcgtcatgt ggaagaccgg cgccgcggcg tacgacgccg gcgtcaaggc gctgctgggg
     3541 ctgcagcccc acgaccacat cgcggctatc ctgcatctgg gaacgcgcct caaatagcgc
     3601 gggcgcgcag cgacaccgcc ttcacgagcg cccacactgg cagtcccgac cgtaatgaca
     3661 gttctcgcgt cgccgccttg gtcaccc
//
LOCUS       DASWZT010000436         9559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_85604, whole genome shotgun sequence.
ACCESSION   DASWZT010000436 DASWZT010000000
VERSION     DASWZT010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9559
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85604"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VGE92_12380"
     CDS             complement(<1..765)
                     /locus_tag="VGE92_12380"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005266030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEY1314672.1"
                     /translation="MYRYDAVDKTLVGERVAQFRDQTRRFLAGELSEDEFRLLRLRNG
                     LYIQRHAPMLRVAVPYGLLSSTQLRALGSIARRYDRGYGHFTTRQNIQFNWPKLEQVP
                     DILAELAEVEMHAIQTSGNCIRNTTSDHLAGVTPDELEDPRPWCELIRQWSTFHPEFT
                     YLPRKFKIAVTGAAQDRAASLVHDVGVHIVRGPTGDLGFEILAGGGLGRTPIIGQVVR
                     EFLPREHLLSYLEAILRIYNLEGRRDNLQKARIKILV"
     gene            914..1417
                     /locus_tag="VGE92_12385"
     CDS             914..1417
                     /locus_tag="VGE92_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314673.1"
                     /translation="MATDLPEVIVAPDTYHVSPEPMLLIADLHGALALCLHDEGRAVG
                     GLLHLQFIGGTGRPSDVTDNTLSSVLVVLDRFKRAVLGNATRGSDVQARILAHALPPT
                     DRGEPSASLVDLIKADLADGKIACGTQTTRRTEPLRVCFQPFEGRVWICGPKNLRAMS
                     RTRGSAV"
     gene            1489..2127
                     /locus_tag="VGE92_12390"
     CDS             1489..2127
                     /locus_tag="VGE92_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314674.1"
                     /translation="MAFLAACAGEKLAQAPPAGVDFSGRWKLNEADSDDPQRLLQAQF
                     DNPTSSQASAPPGGGGGGRGGRGGGRGGGGGPAGPQGPQMPSAGALDEGLRWPGKQLE
                     IKQVAGVVAFTSDGINRVCQPTDGGKSHRPHGDSGDRDAPVRGRDTPPPRCGWTDKTL
                     VVQGGDPDDDHPPFEEHFSLSEDRQRLVEVVGFKGGRSSGFTLSRVWDRSAQ"
     gene            2398..2958
                     /locus_tag="VGE92_12395"
     CDS             2398..2958
                     /locus_tag="VGE92_12395"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY1314675.1"
                     /translation="MAKASKSIVPQIEVRTRRAYFDCKFGQLHVRTAFPTTGGFDEQV
                     TLFCLHALEGSSRDFGHILAALADVRSVYAPDLPGFGESDSSPARSIVDAATATSDLA
                     SGLRLRQIDVLGVQYGAEVALQLAAARPELVRRLVLANLSGTERLPAVKQPRLILDVA
                     RAGDAPFEAAPADLTKQISAFLRETQ"
     gene            2945..3628
                     /locus_tag="VGE92_12400"
     CDS             2945..3628
                     /locus_tag="VGE92_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019301304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY1314676.1"
                     /translation="MRLNKFISETGVCSRREADKWIEAGRVTCNGQLAGLGTKVADGD
                     EICVDGAPVGAKKRPIYIALNKPVGVTCTTEAHIEDNIIDLIGHPERIFPIGRLDKDS
                     EGLILLTNNGDIVNEILRSENNHEKEYLVTVDRPITDLALKMLAGGVKIMGEQTKPCR
                     VERIGRDGFRMILTQGLNRQIRRMCSALGYRAQRLQRVRIMNIHLGTLSSGKWRYLTN
                     AELSGLLPR"
     gene            complement(3634..4089)
                     /locus_tag="VGE92_12405"
     CDS             complement(3634..4089)
                     /locus_tag="VGE92_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314677.1"
                     /translation="MASDSATTISVIDFRRHSMVLQWHSVAGTWSPCETPPALVHGIA
                     LIRSSQPNICVYGQSGRLRLQVGPNQYALSETSPHITCSRGFASFGFRRRFTVRSSSG
                     AILYSHGYWSSQRRDFFRWLATRAEDPDWRATSGRSWSEGLAASVLRAH"
     gene            complement(4108..6240)
                     /locus_tag="VGE92_12410"
     CDS             complement(4108..6240)
                     /locus_tag="VGE92_12410"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEY1314678.1"
                     /translation="MRTLKLWLLGLMLASPAAWALSGNVVATANVKARLASEVSAIAP
                     GQSFWVALEFDIREGWHTYWRNPGDSGQATSLVWRLPPGFTAGDIVWTTPHSFEIPPL
                     MNYGYAGHAVHLVNIVAPKDLKNGTPVELSAKASWLVCSDVCIPEDAELHLTLRVGTV
                     PGAVDPADAQLFSTARGELPSPELGATGARIQGDQLVISLGKEWGATLSQIKSLTFFP
                     YDEGGIEYAAPQKLLRGQNAVELSMKLGDRPPKAGIVRGVLLATEQNGGETVTVPLEV
                     AADFAHGGSGKIAQATSEEKTSVAALLLLAILGGLILNLMPCVFPVLSIKALGLVEQA
                     KKHPAAVRTKGVVFAAGVITSMLLLAAVLLALRAGGEQIGWGFQLQSPLFVTFLVYLL
                     FAVGLNLSGVFEVGGGLAGLGDNLTQGEGYRASFFTGVLTTLVATPCTAPYMAFAVGA
                     ALTQSPFVAMCIFAALGFGLALPYLLLSFAPWLRRALPKPGAWMDTLKQVFAFPIYAS
                     SAWLLWVLAQQTSSFGLAAALAGAILIALAAWAFQKSKSSSAAGRIAALATATLAVLL
                     AIILPVRFGGVAAAASSAAAGGAHTSDEWQPYDAGQVAKLNAAGRPLLVNFTASWCLT
                     CLVNERNAFADSAVQEVFRDKGVTLMKGDWTNRDPAITQALAGFGRAGVPLYVVYKSK
                     PGTTAPVILPQLLTASVVQSAFADTPKP"
     gene            6355..7875
                     /gene="ilvA"
                     /locus_tag="VGE92_12415"
     CDS             6355..7875
                     /gene="ilvA"
                     /locus_tag="VGE92_12415"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEY1314679.1"
                     /translation="MQENYVERILKAKVYDVAVETSLDAAPRLSRRLNNRVLFKREDL
                     QPVFSFKIRGAYNKIAQLSALSAQRGVICASAGNHAQGVALGARTRGIPAVIVMPLTT
                     PEIKVQAVADIGAETMLHGDDYDQAYERAVDVGRERGLVFVHPFDDADVIAGQGTIAM
                     EILRQLHGDSIDAIFVPVGGGGLIGGIAAYAKSLYPRIKIIGVEPEDSAGMYQSLRAG
                     KRVTLDRVGTFADGVAVRRVGEETLRLVRQYVDEIVLVTTDQICAAIQDIFEDTRAIA
                     EAAGALAVAGIKKYVTRENCSARTFVAVNSGANMNFDRLRYVAERSDIGAQREALFAV
                     QIPEVPGSFLHFCELLGRRNVTEFSYRYGDLQAAQIFVGVALTQGKAERAELLQSLAA
                     AGYVVRDMTDNEVAKLHVRHMVGGHARGLADERLYRFEFPERPGALLKFLQAIGTGWN
                     ISLFHYRNHGSDYGRVLAGIQVPVETRTDFLLHLNELHYSYTEETDNPGYKIFLGG"
     gene            complement(7903..>9559)
                     /locus_tag="VGE92_12420"
     CDS             complement(7903..>9559)
                     /locus_tag="VGE92_12420"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1314680.1"
                     /translation="LRGDLDNIVLMAMRKEPERRYGSSQQMAGDIQRYLDGKPVIAHR
                     DTISYRSGKFVRRHWLPVTAVAGAVFMILAFATTTYVQSLRIAAERDRVAEQREHADR
                     ERARAEEVSSFLVNLFKLSDPGENRGNRVTARELLDSGAKRLQAELKDQPETKAALLA
                     TVGAVYDSLGQYQDAMPILNESLQLQARSDDKSRINTMLELGQARVGAGDLGGAEQSL
                     QQALKLSQSNSGPNSLESGRALWALGTLRLQQGRNGDAKDLYIRSLSIMETGGAQQTD
                     ISGVLDDLADAYTRENQWALAKQTYERSLQIDRRALGEDHPRVARLLNNLAVVAQNMG
                     DLPQSERLYNEALRRHELTFGERHPETAGIMGNLGLLLQREGRLAEAEPLLRNALDLN
                     LSIYGPANYNVGYSRVSLAMLLHDQGKFAPAETEFRQALAIYDQSLPADHQYRASALM
                     HFARLLVDRGKLDEALKLSQESINIWTATSPALSPTTAQAHTIHAYALSHLGRTHEAA
                     DELEAALPILTKSRGVDDPGVRRAQAWLKAVRPVPVQTASAAR"
BASE COUNT         1875 a         2932 c         2980 g         1772 t
ORIGIN      
        1 caccagaatc ttgatacggg ccttttgcag attatcgcgg cgcccctcga gattgtagat
       61 cctcaatatg gcttccaaat acgacagcag gtgctcacgc ggcaggaact cgcgcaccac
      121 ttgaccgatg atgggcgtgc gtccgagacc gccgccggcc agtatctcaa accccaaatc
      181 gcccgtgggg ccgcgcacga tgtgcacacc gacatcgtgg accagcgagg cagccctatc
      241 ctgcgcagcg cccgtgacgg caatcttgaa ctttcgaggt aggtaggtga actctgggtg
      301 gaacgtcgac cactggcgaa tcagttcgca ccaggggcgc ggatcctcga gttcgtcggg
      361 tgtcacgccc gccaggtggt cggaggtcgt gttgcggatg cagttgccgc tggtctggat
      421 cgcgtgcatc tcgacctcgg cgagctcagc caagatatcc ggtacttgct cgagcttggg
      481 ccagttgaac tggatgttct ggcgcgtcgt aaaatggcca tagccgcgat cgtagcgccg
      541 cgcgatgctg ccgagggcgc gcaattgcgt cgacgacaag agcccatacg gaacagccac
      601 tcgcagcatg ggcgcatggc gctggatata caggccgttg cgcaggcgca agaggcgaaa
      661 ttcatcctca ctgagctcgc cggcaaggaa acggcgtgtc tggtcgcgga attgtgcgac
      721 gcgttcgccc accagcgttt tgtcgactgc gtcataccgg tacatcgcgc cacgatacgt
      781 gcggcacagc cgagccgcca attaccggaa tgcatgagct tagacccact cgtcctagcg
      841 gaaaccgtga ttggctcggc agagggaatt cgcagttaaa ggcagcatca gggaggtcaa
      901 ttaggggtcc gcattggcta ctgatcttcc ggaagtcatc gtcgcgccgg acacttatca
      961 tgtgtcgcct gaacccatgc ttttgattgc cgatctacac ggcgcactgg cgctgtgtct
     1021 gcatgacgag gggcgcgcgg tgggtggcct gttgcatctg cagttcatcg gtggaaccgg
     1081 tcgcccgagc gatgtcaccg acaacacctt gagctcggtg ttggtggtgc tcgaccgctt
     1141 caagcgcgcg gtgctcggca acgccacacg cggttccgat gtccaggccc gtatcctcgc
     1201 gcatgcgttg ccgcccaccg atagaggtga gcccagtgca tctctcgtgg acttgatcaa
     1261 ggccgatctt gccgacggca aaattgcatg cggcactcaa accacccgca gaaccgagcc
     1321 gctgcgtgtg tgctttcagc cgttcgaagg tcgggtgtgg atctgcggac caaagaatct
     1381 gcgcgccatg tcccgaacac gcggttccgc cgtatagcac tcccgagata cccgcgggag
     1441 cgggcttcat tataagattc ggggatgcga ttctcgttcc ttctggcagt ggcattcctc
     1501 gcagcctgtg cgggcgaaaa gcttgcccag gcgccccccg ccggcgtcga cttcagcggc
     1561 cgctggaagc tcaacgaggc ggatagcgat gacccgcagc gcctgctgca ggcgcaattc
     1621 gacaacccga cgagcagcca agcgagcgca ccgcccggcg gcggtggcgg cggtcgaggt
     1681 ggccgaggag gcggtcgcgg cggcggcggc ggtccggcgg gacctcaggg tccgcagatg
     1741 ccgtcggctg gcgctctgga cgagggctta cgctggcccg gcaagcaact cgaaatcaag
     1801 caagttgccg gcgtggtggc attcacgtcg gacggtatca accgcgtctg ccaaccgacg
     1861 gacggcggta aatctcatcg gccgcatggc gactcggggg atcgcgatgc acccgtccgg
     1921 ggacgagaca cgccgccgcc gcgttgcggg tggaccgaca agaccctggt ggtacagggc
     1981 ggcgatccgg acgatgacca tccgcctttc gaggaacatt tcagcctgtc cgaggaccgg
     2041 cagcgcctcg tcgaagtggt gggattcaag ggagggcgct ccagtggatt cacgttgtcg
     2101 cgggtttggg accgctcggc gcaataaaaa agccgggcat tgcccggctt tcaagtcccc
     2161 atgcctttgt gtggcgctta ttcgcgctct ggcgatgatt ggtcgtttta cttccctaca
     2221 atcctattgc gtccgagcaa cactctagcc ttgcgccgcc gccgaagcta gcccaattgc
     2281 aaagaagtgc aaaacttaat gtagcaaaaa aacaacaaag gcgcgagtcg ctccgcgacg
     2341 cagcagcgac ttcgcgtatc cttgtagacc gctgcttgct caagctggag acctaccgtg
     2401 gctaaggctt cgaagtcaat cgttccgcag atagaagtcc ggactcggcg ggcctatttc
     2461 gattgcaaat tcggccagtt gcatgtgcgc acggcttttc cgacgacagg tggattcgat
     2521 gagcaagtca ctttgttctg tctgcatgcc ctcgagggct cgagccgcga tttcggccat
     2581 atcctcgccg cgctcgccga cgtgcgttcg gtttacgccc cggatctacc agggttcggt
     2641 gaatccgatt cctcgcctgc acgcagcatc gtggacgccg ccacggcgac ttccgacttg
     2701 gccagcggct tgcgattgcg tcaaatcgac gtgctcgggg tgcaatatgg cgccgaggtt
     2761 gcattgcagc tcgctgcggc acgacctgaa ctcgtcaggc gcctggtgtt ggccaaccta
     2821 tcgggcacgg agcgcctgcc ggcggtgaag cagccgcgac tgatcctcga tgtcgcccgc
     2881 gccggggacg cccctttcga ggccgcccca gccgatttga cgaagcaaat cagcgcgttc
     2941 ctccgtgaga ctcaataaat tcatcagcga aaccggggtc tgttcgaggc gcgaagcaga
     3001 caagtggatc gaggcggggc gcgtcacttg caacggtcag ctcgccggtt tgggcaccaa
     3061 ggtggccgac ggagacgaga tttgcgttga cggcgcaccc gtcggcgcca agaaacggcc
     3121 gatctacatt gccctcaaca agccggtcgg cgtgacttgt accaccgaag cacacatcga
     3181 ggacaacatc atcgatttga tcgggcaccc cgagcgaatc tttcccatcg gccggctcga
     3241 caaagactcg gagggcttga ttctgttgac gaacaacggc gacatcgtca acgagatcct
     3301 gcgctccgag aataatcacg aaaaggagta cctcgtgacc gtggatcgtc ccatcactga
     3361 cctggcgctg aaaatgctgg caggcggcgt aaagatcatg ggcgaacaga caaagccttg
     3421 ccgagtcgag cgcatcggcc gggacggatt tcgaatgatc ctcactcaag ggttgaaccg
     3481 ccaaatccgc cgcatgtgtt cggcgcttgg ctatcgagca caacggctgc agcgcgtgcg
     3541 catcatgaac attcacttgg gaacgttgag cagcggcaag tggcgctacc tgacgaacgc
     3601 ggagctcagc ggccttttgc cgcgctgaag cgctcagtga gcccgcagca ccgaggcggc
     3661 gagaccttcc gaccagctgc gcccgctggt ggcccgccaa tcaggatcct cggcgcgagt
     3721 cgcgagccag cgaaagaagt cgcggcgttg gctcgaccag tagccgtgac tgtagagtat
     3781 cgcgccgctg ctcgaacgga cggtaaaacg gcggcgaaag ccgaagctcg cgaatccccg
     3841 cgaacaggta atgtgcggcg aagtttccga cagagcgtac tggttggggc cgacttgcag
     3901 gcgcaggcgc ccggactggc catacacgca gatgttgggt tgcgatgaac gtatcaaggc
     3961 aatgccatga accagcgccg gcggggtctc gcaggggctc cacgttcccg cgaccgaatg
     4021 ccactgcagc accatgctgt gacggcggaa gtcgattacc gaaattgttg ttgcgctatc
     4081 ggaagccatg cccgcagcat gccgcattca tggcttgggt gtatcggcga atgcgctttg
     4141 caccacactg gccgtcaaca gttgcggcag tatgacgggc gcggtggttc cgggcttcga
     4201 cttatacacc acgtagagcg gcacgccggc acgtccgaaa cctgccagcg cctgggtgat
     4261 ggcgggatct cgattggtcc aatcgccctt catcagggtg acgcccttgt cccggaagac
     4321 ctcttggacc gcggaatcgg caaatgcatt gcgctcgttg accaagcagg tgaggcacca
     4381 actggcggtg aaattcacca gcagcggccg tccagcggca ttcaacttcg cgacttgtcc
     4441 cgcatcgtaa ggttgccact catcggacgt atgggcgccg cccgccgccg cgctcgatgc
     4501 tgccgcggcg acaccgccga agcgcaccgg caatatgatg gccagcagca ccgcaagcgt
     4561 ggccgtggcg agcgcggcga tacggcctgc cgccgagctc gacttcgatt tctggaaagc
     4621 ccaggccgcc aacgcgatca ggatggcgcc cgccaatgcg gcggccaagc cgaaggagga
     4681 agtctgctgc gcgagcaccc acagcagcca cgccgacgag gcgtaaattg ggaatgcaaa
     4741 tacctgtttg agcgtgtcca tccaggcgcc gggttttggc agcgcacggc gcagccacgg
     4801 agcgaacgac aacagcaaat agggcagcgc gagtccgaaa ccgagggcgg cgaaaatgca
     4861 catcgcaacg aacggcgact gggtcagggc ggcgccgacc gcgaatgcca tgtacggcgc
     4921 ggtacatggt gtcgcgacca atgtagtcag cacgccggtg aagaacgagg cgcggtagcc
     4981 ctcgccttga gtgaggttgt cgccgagacc ggcgagtccg ccgccgactt cgaagacccc
     5041 cgagagattc aggcccaccg cgaatagcag gtacaccaag aacgtgacga acagcggcga
     5101 ttgcagctga aaaccccagc cgatttgttc tccgcccgca cgcaatgcca ggagtaccgc
     5161 ggcgaggagc agcattgagg tgatgacgcc cgccgcgaac accacgccct tggtgcgcac
     5221 ggccgcggga tgctttttcg cctgttcaac caagcccagc gctttgatcg acagcaccgg
     5281 gaaaacacac ggcattagat ttaggatcag gccgcccaag atggccagca gcaacaatgc
     5341 agccaccgaa gtcttttcct cgctcgtagc ctgcgcaatt ttcccgctgc cgccgtgcgc
     5401 gaagtctgcg gcgacttcca gcggcacggt caccgtctcg ccgccgttct gttcggtcgc
     5461 caacaacact ccgcgaacga ttcccgcctt gggcgggcga tccccgagtt tcatggacag
     5521 ttcgaccgcg ttctggccgc ggagaagttt ctgcggtgcc gcgtactcga tgccgccttc
     5581 gtcgtatggg aagaacgtca gcgacttgat ctgcgacagc gtggcgcccc actcttttcc
     5641 gagcgagatc accagctggt cgccctggat ccgcgcgccg gtcgcgccaa gctcagggct
     5701 cggcagttcg ccacgtgcgg tcgagaacag ctgcgcatcc gccggatcga ccgccccggg
     5761 gacggtgcca acccgcagtg ttaaatgcag ttcggcatct tcgggaatgc acacgtccga
     5821 acagaccaac cagctggctt ttgcggacag ctcgacgggc gttccgttct tcaagtcctt
     5881 gggcgcgact atgttgacca ggtgcaccgc atgtccggca tagccgtagt tcatcagcgg
     5941 cggaatctca aaactatgcg gagtggtcca aacaatgtcg ccggcagtga atccaggcgg
     6001 cagccgccac acgagcgagg ttgcctggcc cgagtcgccg ggatttcgcc aatacgtgtg
     6061 ccagccctcg cgaatatcga attccagagc aacccagaag gactggccgg gagcgattgc
     6121 actgacttcg ctcgccaagc gcgccttcac atttgcggtg gcgaccacgt ttccggacag
     6181 cgcccaggcg gcaggggagg cgagcatcaa gcccaacagc cacaatttca gggtgcgcat
     6241 gagcgtatgg taccgaatta aatgtagcga aacccgcgcg ctccgcgggc tgtgcagcat
     6301 ctcacagaat gcatcgacca taccgcttcg cggacgcttg agctaaggta agtaatgcaa
     6361 gaaaactacg tagaacgcat cctcaaagca aaagtatacg acgtcgcggt cgagacctcg
     6421 ctggacgccg cgccgcggtt gtcgcgtcgc ttgaacaatc gagtcttgtt caaacgtgaa
     6481 gacctgcagc cggtgttttc gttcaaaatt cgcggtgcct acaacaagat cgcccagctc
     6541 tcggctctca gcgcgcagcg gggcgtgatc tgcgcgtcgg ccggcaacca tgcgcagggc
     6601 gtggcattgg gcgcccgcac gcggggaatt ccggcggtca tcgtcatgcc gctgaccacg
     6661 cccgagatca aagtgcaggc cgtggcggat atcggcgcgg agaccatgct gcatggcgat
     6721 gactatgacc aagcgtacga gcgcgcggtc gatgtcggcc gcgagcgcgg actggtgttc
     6781 gtgcaccctt tcgacgacgc ggacgtgatt gccgggcaag gtacgatcgc catggaaatc
     6841 ttgcgtcagt tgcacggcga ttccattgac gccattttcg tgccggtggg cggcggtgga
     6901 ctcattggcg gaatcgccgc ctatgcgaaa tccctgtacc cgcgcatcaa gatcattggc
     6961 gtcgagccgg aggactcggc aggaatgtac caatcgttgc gtgccggcaa acgcgtgacg
     7021 cttgatcgcg tgggtacgtt cgccgacggc gtcgccgtgc ggcgcgtcgg cgaggagacc
     7081 ctgcggttgg tgcgccaata cgtggatgaa atcgttctgg tgaccaccga tcagatatgc
     7141 gcagccatcc aggacatttt cgaggacacg cgcgccatcg ccgaggcggc gggcgctctc
     7201 gccgtcgccg ggatcaagaa atacgtgacg cgagaaaatt gcagtgcacg caccttcgtc
     7261 gccgtcaata gcggcgccaa catgaacttc gaccggttgc gctatgtggc cgagcgctcc
     7321 gatatcggtg cgcagcgcga agcgctgttc gccgtgcaaa tccccgaggt accgggttcg
     7381 ttcctgcact tctgcgagct gctcgggcgg cgcaacgtca ctgaattcag ctatcgttac
     7441 ggcgacttgc aggcagcgca aatcttcgtc ggtgtcgcgc tcacccaggg caaagcggag
     7501 cgcgccgagt tgctgcagag cctcgccgcg gccggctacg tggtgcgcga catgaccgac
     7561 aatgaagtgg cgaaattgca cgtgcgccac atggtggggg gccacgcgcg cgggctcgcc
     7621 gacgagcggt tatatcgatt cgaattcccg gagcgccccg gagcgctgct caagttcctg
     7681 caggctatcg gtaccgggtg gaacatcagc ctgttccact accgtaatca tggctcggac
     7741 tacggccgcg ttctcgccgg cattcaggtg cctgtcgaaa cccgcaccga ctttcttttg
     7801 cacctcaacg aactgcatta ctcctatacg gaagagaccg acaatccggg ctacaagatt
     7861 tttctcggcg gctgacccaa gggcggccga taggggcaat ggctagcgtg cggcgcttgc
     7921 ggtctgcacg ggcaccggac gcaccgcttt cagccacgct tgagcgcggc gcacccccgg
     7981 atcgtcgacg ccgcgtgact tggtcaatat cggcaacgcc gcctcgagtt cgtcggcggc
     8041 ctcgtgagtt ctgccgagat gcgagagcgc ataggcatga atggtgtgcg cttgcgcggt
     8101 cgttgggctt aaggcgggcg aagtagccgt ccaaatgttg atcgactcct ggctcaattt
     8161 taaggcttcg tcgagcttgc cgcgatccac cagcagtcgc gcgaaatgca tgagagccga
     8221 ggcacgatat tgatgatcgg cgggcagcga ttggtcgtaa atggcgagcg cctgccggaa
     8281 ctcagtctcc gccggcgcga attttccttg atcgtgcagc agcatcgcca agctgacgcg
     8341 ggaataaccc acgttgtagt tggcgggacc ataaatggac aggttgaggt cgagcgcatt
     8401 gcgcaacagc ggttctgctt ccgccaatcg tccttcacgc tgcaacagca aaccgagatt
     8461 tcccatgatt ccggcggttt cgggatgccg ttctccgaag gtgagctcgt gccgacgcag
     8521 ggcctcatta taaaggcgct cggattgcgg tagatctccc atgttttgcg cgacgactgc
     8581 caaattgttg agaagcctgg ctacccgagg atgatcctcg ccaagcgcgc gtcggtcgat
     8641 ctgaagcgac cgctcataag tttgcttcgc cagcgcccat tgattctcgc gcgtgtaggc
     8701 atccgccaag tcatcgagca cgccggaaat atccgtttgc tgagcgccgc ccgtttccat
     8761 gatactgagg ctgcggatgt acaaatcctt ggcgtcgcca ttccgaccct gttgcagcct
     8821 gagcgttcca agtgcccaca gcgcgcggcc cgattccaag ctgttgggtc cggagttgct
     8881 ctgcgaaagc ttgagggcct gttgtagaga ctgctccgct ccgccgaggt cgcccgcgcc
     8941 aacgcgggct tgaccgagtt ccaacatggt attgatacgg gacttatcgt cggaccgcgc
     9001 ctgcagctgc aaggactcgt tgagtatcgg catcgcgtcc tggtactgtc ccaggctgtc
     9061 gtaaacggcg cccactgtag cgagcaatgc cgctttggtt tcgggttgat ccttgagttc
     9121 ggcttgcaat cgtttcgcac ccgaatccaa cagttcacgg gccgtgactc ggttgccgcg
     9181 attctccccg ggatccgata gcttaaagag attcacgagg aagctagaca cttcttcggc
     9241 ccgagcgcgc tcgcgatccg catgctcgcg ttgttcggcg acgcggtcgc gctccgctgc
     9301 gatgcgtaag gactgcacgt aggtcgtcgt cgcgaaggcc aaaatcatga agaccgcgcc
     9361 ggcgaccgca gtcaccggca gccaatggcg ccgcacgaac ttcccactgc gatacgagat
     9421 ggtgtcgcga tgcgcgatga caggtttgcc atccaagtag cgctggatat ccccggccat
     9481 ctgctgcgag gagccataac ggcgttccgg ctccttgcgc atggccatca acacgatgtt
     9541 atccaagtct ccgcggagg
//
LOCUS       DASWZT010000437         8969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_71185, whole genome shotgun sequence.
ACCESSION   DASWZT010000437 DASWZT010000000
VERSION     DASWZT010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8969
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71185"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VGE92_12425"
     CDS             complement(<1..744)
                     /locus_tag="VGE92_12425"
                     /inference="COORDINATES: protein motif:HMM:NF039283.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HEY1314681.1"
                     /translation="MTTASLDLAVIGNCQVAALIDSQGRIVWGCLPKPDADPVFCALL
                     SRAGGASSSGVFAVDLRDMTDAKRIYLRNTAIVETTLSDAHGNSLKITDFCPRFRSRG
                     RNFRPMAFVRLVEPIRGRPIVTVRLRPRCDNGADIPQRVAGSHHIRFLSAALNFRITT
                     NSSLPPLLDESAFVLGTPLAFILAPDETIEETPLTLAISWLDETRNYWHEWIRGLAVP
                     FDWQEAVIRSAITLKLCTFEDTGAVMAALT"
     gene            complement(741..1445)
                     /gene="otsB"
                     /locus_tag="VGE92_12430"
     CDS             complement(741..1445)
                     /gene="otsB"
                     /locus_tag="VGE92_12430"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEY1314682.1"
                     /translation="MDVDGTLIEFTDSPHDTCADAELKALLGEVAERLGGAVALVSGR
                     SIEYLDSLFAPLRLPAAGLHGVERRKASGVMHGASFVDSQLDRARAELKALVTANPGT
                     VLEDKGRTLAVHFRMAPQTEATVRDAVRGLATPLGSNYHIQGGNMMLEIKPSGFTKAA
                     AIKAFMQEPPFSGRTPVFVGDDLTDQEGLTAVEDQGGISVAVGERVQGQYHLENAAAV
                     RIWLLDIAALQDSHRE"
     gene            1613..3847
                     /locus_tag="VGE92_12435"
     CDS             1613..3847
                     /locus_tag="VGE92_12435"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEY1314683.1"
                     /translation="MFRSVMLRFVLPVAFASCVVAYFGLPYIERLLAEWFRSDIELRA
                     QLVMHSMEEPITDLVEKGNEARLRSYLAKITADERLLAILVCRPNGSTMFKSERTPAS
                     ISCDLTDTLSPAASRIMQLPSGSVQVSRFDFDSAEPTPFRVLMLHDLSFVDRRQRTAR
                     DFLLVFVGISVLLLALLIVLVAWLQLRRFAQVLIGDIRGKRFLDDARSPRASLPILSQ
                     VRKVLAEAEETQRLEIDFHENWTPQALRQVVRDHLHSAQVIIVSNREPYIHNFDANRH
                     PVMQVPASGMVTALEPIMRACSGVWVAHGAGTADRETVDRYDQIRVPPDDPSYTLRRV
                     WLTEEQEQGYYYGFSNEGLWPLCHLAYVRPAFRASDWHAYEEVNAKFADVVAAESSSD
                     SPVVLIQDFHFALLPQLIRKKIPKATIALFWHIPWPNAETFGVCPWKREMLMHMLSAD
                     ILGFHTRYHCQNFLGSVDRFVECQIDHEHMTVTLQGHVCRVAPYPISIEWPPRWLKGL
                     PDVATCRTAVRERHRIGADVVIGLGVERWDFTKGIIERFHALETLLDRNPRHRGRICL
                     LQVAAPSRSQLAAYQALQQQTYAEVERINAKFAEGDWRPIVLIDEHQDPPRIFELYRA
                     ADFCLVNSLHDGMNLVAKEFVAARDDEDGVLILSTFAGASRELAEAVLINPFDVTETA
                     EAMQIAMRMGRDERRNRMSLMRRTVKENNVYRWAGRMLMDAARIRQRQSLTSAPRRYA
                     TTDR"
     gene            complement(3844..5004)
                     /locus_tag="VGE92_12440"
     CDS             complement(3844..5004)
                     /locus_tag="VGE92_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009485198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HEY1314684.1"
                     /translation="MRFDSKLPDVGTTIFTVMSRRALEEGALNIGQGFPDYPIDSRLA
                     AALTAVVGEGHNQYAPMEGVIALREQIAAKLYKSYALSFDPQSEITVTCGGTEALYDA
                     IQAVVGPGDEAIIFDPAYDAYEPAVRLAGGRCIRIALAPPAFRFDWDEVRRSLSDRTR
                     LIIINSPQNPSCTVAERDDLDALATVIRDRAITVLADEVYEHVLYDGRRHASVLAHAE
                     LRERSFAVFSFGKTLHATGWRIGYCVAPPALTRELRKVHQFNSFSIVSPLQYAIARYL
                     ESYPDAWAGLAAFFQAKRDLLVRHLHGSGFKPIVAAGTYFQLVDYGALSRDTDMNFAD
                     RLIREAKIATIPLSPFYATPPAMTLLRLCIAKRDATLEAAAERLCAFADAVG"
     gene            complement(5007..6050)
                     /locus_tag="VGE92_12445"
     CDS             complement(5007..6050)
                     /locus_tag="VGE92_12445"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEY1314685.1"
                     /translation="MQPDYSRQTLLKEIGASGQAALGRARVLVVGAGGLGSPVLQYLA
                     GAGVGCLGIVDADALEASNLHRQPIFSLADAGAPKVELARAAVQRINPSVQVELHASR
                     LDASSALELIRAYDVVIDCTDNFRSKYLINDAAVIARRPAIFASVYQYEGQLQVYKPE
                     MRHACLRCLWPDAIADGVVGNCAEAGVLGPVPGTFGTLQALLALKILLRLPGQLAGEL
                     LLVDFTNFSLLKLKAPRRAECRAPDCAHIREIAGEEPGIEIALGSLSQAAMHGLEVID
                     IRSDEEFAAQPTAARHIPMSALLADPHLLLAAREFLLVCASGKRSLAAARELRQRGLA
                     VRSLAGGLRSLEN"
     gene            complement(6056..6526)
                     /locus_tag="VGE92_12450"
     CDS             complement(6056..6526)
                     /locus_tag="VGE92_12450"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEY1314686.1"
                     /translation="MQFRLCQTAIDTGRARRELLDLGAGGYVAFEGWVRDHNEGQEVS
                     RLEYEAFEELAVKEGERIIAEALRRFPVRHALCIHRVGTLSLSDMAVWVGVSSTHRGE
                     AFDACRFIIDEVKHRVPIWKKEHYRSGDSGWVNCERCAATPGHTHEPVALANPK"
     gene            complement(6526..6765)
                     /locus_tag="VGE92_12455"
     CDS             complement(6526..6765)
                     /locus_tag="VGE92_12455"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY1314687.1"
                     /translation="MQLSIHYYALMREQAGRSEETLETAAATPSALYDELSARYGFTL
                     AREQLKVAVNSEFSDWSRRLTAGDAVVFIPPVAGG"
     gene            complement(6775..7380)
                     /locus_tag="VGE92_12460"
     CDS             complement(6775..7380)
                     /locus_tag="VGE92_12460"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEY1314688.1"
                     /translation="MKSAAAPAVYGLILAGGSSSRMHRDKAALRYGGKHQLDRAFDLT
                     SRHVATVFASVRASQTADPTRAQRPMIVDSFPGEGPIVGIRSALAAHPQVAWLVVACD
                     LPFLSDTALSQLLRERDAAALATAFRSAHDGLPEPLCAIWEPQAAAALMSYQDGGGQC
                     PRKFLLRHGAHLLEPLDRRALDNVNTPEEYAHASSTFDTSL"
     gene            complement(7377..7856)
                     /gene="moaC"
                     /locus_tag="VGE92_12465"
     CDS             complement(7377..7856)
                     /gene="moaC"
                     /locus_tag="VGE92_12465"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF006870.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEY1314689.1"
                     /translation="MTFTHLDSDRHPAMVDVSDKKASKRTATAEAQVRFPAAVAAALR
                     DSGLSSPKGPVFDTAIVAGVMGAKRTHELIPFCHPLGLENCRVSIELEDDTAVIRCTV
                     SVHHKTGVEMEALTGASIAALTIYDMCKALSHDIVISGVRLLAKDGGRSSYALKATP"
     gene            complement(7861..8910)
                     /gene="moaA"
                     /locus_tag="VGE92_12470"
     CDS             complement(7861..8910)
                     /gene="moaA"
                     /locus_tag="VGE92_12470"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEY1314690.1"
                     /translation="MSSTVHHIHGPKDPLDRLGRPLHDLRISVMDRCNFRCPYCMPKE
                     QYHEHYRFLKSQERLSFDEIVRLARLFANLGVRKLRLTGGEPLLRTNLADLIGDLSDV
                     PGIDDIALTTNGVLLGQHAVDLHANGLRRVTVSLDTLDKEVFARMSGGYHALDQVLHG
                     IDCAIAAGLTPVKVNAVIERGLNDHTALDLLERFRGSPVIVRFIEFMDVGNRNNWRPD
                     LVVPSRELAARVHARWPIHPISQNYTGEVAQRWRFDDGAGEVGFISSVSQPFCGACSR
                     ARLSSEGKFYTCLFATQGLDLRAPLRAGAADADLLQLIRGVWHGRTDRYSEQREELRR
                     VKPETKKIEMNYIGG"
BASE COUNT         1725 a         2763 c         2819 g         1662 t
ORIGIN      
        1 ggtgagagcc gccatcaccg cgccggtgtc ttcgaaggtg cacaatttca atgtgatcgc
       61 cgagcgaatc accgcttcct gccaatcgaa gggcaccgcc aggccgcgaa tccattcgtg
      121 ccagtaattg cgtgtctcat cgagccagga tatcgccaag gtcaacggcg tctcctcgat
      181 ggtttcgtcc ggggcgagga tgaacgcgag cggggtaccg agcacgaagg cgctctcgtc
      241 gagcagcggc ggcagcgagc tgttggtcgt gatgcggaaa ttgagcgccg cggataggaa
      301 gcgaatgtga tggctgccgg cgacccgttg cgggatgtcg gcgccattgt cgcagcgcgg
      361 ccgcaggcgc accgtgacga tgggccggcc gcgaatgggt tcaactagcc gcacgaaggc
      421 catggggcga aagttacggc cgcgcgaacg aaaacgcggg cagaaatccg tgatcttcag
      481 tgagttgccg tgggcatcgc tcaacgtcgt ttcgacaatc gcggtgttgc gcagatagat
      541 gcgcttcgca tcggtcatgt cgcgcaaatc gacggcgaaa acgccgctcg acgatgcgcc
      601 acctgcgcgc gagagcagtg cgcaaaatac cggatctgcg tccggcttcg gcagacagcc
      661 ccagacgatg cggccctgac tgtcgatcaa tgcggcgact tggcagttgc cgatgacggc
      721 gagatccaga ctggcggtag tcattcgcgg tgggaatctt gcagtgcggc gatgtccagc
      781 agccagatgc gcacagccgc ggcattttcc aggtgatact ggccttgtac ccgctcgccg
      841 accgcgaccg aaatgccgcc ctgatcctcg accgccgtca ggccttcctg gtcggtgaga
      901 tcgtcgccca caaataccgg cgtcctgcct gaaaacggcg gctcctgcat gaacgccttg
      961 atggcggccg ctttggtgaa gccgcttggt ttgatctcga gcatcatatt gccgccctgg
     1021 atgtggtagt tgctgccgag cggtgtcgcg agtccgcgca ccgcgtcgcg caccgtagct
     1081 tcggtttgcg gcgccattcg aaagtgcacc gcgagggttc gacccttgtc ctcgagcact
     1141 gtgccggggt tcgcggtcac caacgccttg agttcggcgc gcgcccggtc gagttgcgaa
     1201 tcgacgaagc tcgcaccgtg catgacgccg gacgccttgc gccgctcaac gccgtgcaaa
     1261 cctgcggcag gcagccgcaa cggcgcgaac agcgaatcga ggtactcgat gctgcgcccg
     1321 ctcaccagcg ccaccgcacc gcccaggcgc tcggccacct cgcccaacag cgccttgagc
     1381 tccgcgtccg cgcatgtgtc gtgtggtgag tcggtgaatt caatcagagt tccatcaaca
     1441 tccaagaaca aacaccattc aagtgaaggc gacggcggtg tttggatcat acgacaaagc
     1501 ttaccggatg ggcgtcaacg atggcttacg gctttgcagc cgtgtaagtc ttacacgaaa
     1561 atcatagcgg aaaaattgac accgaaacac cgaaattggg tattttggaa cgatgtttcg
     1621 ctccgtcatg ttgcgtttcg tcctgccggt ggcattcgcg tcgtgcgtgg tggcgtattt
     1681 tgggttgccc tacatcgagc ggctgctggc ggagtggttt cgctcggaca tcgaattgcg
     1741 tgcgcagctc gtgatgcatt cgatggaaga gccgataacc gatctcgtcg aaaagggcaa
     1801 tgaggcgcgg ctgcgcagtt atctcgccaa gataaccgcc gacgagcgct tgctggcgat
     1861 cttggtgtgc cgccccaacg gctcgaccat gttcaagtcc gagcgcacgc cggccagcat
     1921 ttcatgcgac ctcaccgaca ccttgtctcc cgccgcctcg cgcatcatgc agctgccgtc
     1981 cggttctgtg caggtatcgc ggtttgattt cgacagcgcg gagccgacgc cgtttcgcgt
     2041 gttgatgctg cacgatctga gcttcgtcga tcgccgccaa agaaccgcgc gcgatttcct
     2101 gttggtgttc gtcggcatat cggtattgct gttggcgctg ctgatcgtcc tggtggcgtg
     2161 gctgcaatta cggcgcttcg cccaggtgct gattggcgat atccgcggca agcgatttct
     2221 cgacgatgcc aggtcgccgc gcgcgtcgtt gccgatactt tcgcaagtgc gcaaggtgct
     2281 ggcggaagcc gaggaaacgc agcgcctgga gatcgatttt cacgagaatt ggacgccgca
     2341 ggccctgcgg caggtggttc gcgatcactt gcattcggcg caggtcatca tcgtgtcgaa
     2401 tcgcgaaccc tacattcaca acttcgacgc caaccggcac cccgtcatgc aagtcccggc
     2461 gagcggcatg gtgacggcgc tcgagcccat catgcgcgcg tgttccggag tgtgggtggc
     2521 gcatggcgcc ggcaccgccg atcgcgagac ggtggaccgc tacgatcaga ttcgggtgcc
     2581 gcccgatgat ccatcctaca cgctgcgccg cgtgtggctg accgaggagc aggagcaggg
     2641 atattactac ggattttcca atgaaggatt gtggccgttg tgccatttgg cctacgttcg
     2701 gccggctttc cgcgccagcg attggcacgc ctacgaagag gtcaacgcga aattcgccga
     2761 cgtggtggcg gccgaatcga gcagcgacag tccggtagtg ctgatccagg acttccattt
     2821 cgcgctgctg ccgcaactca tccgcaagaa aattcccaaa gcaacgattg ccctgttctg
     2881 gcatatccca tggcccaatg ccgagacttt cggggtatgt ccgtggaaac gcgagatgct
     2941 gatgcacatg ttgtccgcgg acatcctggg atttcacacc cgctatcact gccagaattt
     3001 cttgggctcg gtcgaccgct tcgtcgaatg ccaaatcgat cacgagcaca tgaccgtgac
     3061 tttgcaagga catgtttgcc gcgtcgctcc gtatccaatc tcgatcgagt ggccgccgcg
     3121 ctggctgaag ggcctgcccg acgtcgccac ctgccggacg gcggtgcgcg agcggcatcg
     3181 cattggggcg gatgtggtga tcggcttggg cgtggaacgc tgggacttca ccaagggcat
     3241 catcgagcgc ttccacgccc tcgaaacact gctggacaga aatccgcggc accgcggcag
     3301 gatctgcttg ctgcaggtgg ccgcgccgtc acgcagccag ttggcggcgt atcaggcgct
     3361 gcagcagcaa acctacgccg aagtggagcg catcaatgcc aagttcgccg aaggcgactg
     3421 gcggccgatc gtgctgatcg acgagcacca ggacccgccg cgcatcttcg agctgtaccg
     3481 ggccgcggac ttctgcctgg tcaacagtct gcacgacgga atgaacctcg tggccaagga
     3541 atttgtcgcg gcgcgcgacg acgaggacgg cgtgctgatc ttgagcacct ttgcgggtgc
     3601 atcgcgggaa ctggccgaag ccgtgttgat caatccgttc gacgtcacgg agacggcgga
     3661 ggcgatgcag atcgcgatgc gcatggggcg cgacgaacgg cgcaatcgca tgagcctgat
     3721 gcggcgcacc gtcaaagaga acaatgtgta tcgttgggcg gggcggatgt tgatggatgc
     3781 ggcgagaatt cggcagcggc aaagcttgac ctcggcgccg cgccgctacg ccaccacgga
     3841 tcgctaacca accgcgtcgg caaacgcgca cagccgctcg gccgccgcct ccagagtggc
     3901 atcgcgtttg gcgatgcaca gccgcagcag ggtcatcgcc ggcggcgttg cgtagaacgg
     3961 cgacagcggg atggtcgcga tcttcgcctc gcgaatcagc cgatcggcga agttcatgtc
     4021 cgtgtcgcga ctgagcgcgc catagtcgac caattgaaag tacgtgccgg ccgccacgat
     4081 gggtttgaaa cccgagccgt gcaggtgccg caccagcaga tcgcgcttgg cttgaaagaa
     4141 cgccgcgagc cccgcccacg cgtcggggta gctttcaaga tagcgcgcaa tggcgtattg
     4201 cagcggcgag acaatgctga agctgttgaa ttggtgcacc ttgcgcaact cgcgcgtcaa
     4261 tgccggcggc gccacgcaat agccgattcg ccagccggtg gcatgcaagg tcttgccgaa
     4321 agagaatacg gcgaagctgc gctcgcgaag ttctgcatgc gccaggaccg acgcatggcg
     4381 ccggccgtcg tacagcacat gttcgtagac ctcgtcggcc agaaccgtga tggcacgatc
     4441 gcggatcacc gtggcgagcg cgtccaaatc atcgcgctcc gcgacggtgc aactcgggtt
     4501 ctgtgggcta ttgatgatga tcagccgggt gcgatcgctc aaggagcggc gaacctcgtc
     4561 ccagtcgaaa cgaaacgcgg gtggcgctag cgcaatgcgg atgcaacgcc cacccgccaa
     4621 gcgcacggcg ggttcgtagg catcgtaagc gggatcgaag ataatggctt catcgccggg
     4681 gccgaccacc gcctggatcg cgtcgtacag cgcctcggtg ccgccgcaag tcaccgtaat
     4741 ctcggactgc gggtcgaaac tcaatgcgta gctcttgtag agcttcgcag caatctgctc
     4801 gcgcagcgca atcacgcctt ccatcggcgc atactggttg tgcccttcac cgacgacggc
     4861 agtgagcgcg gcggccaagc gcgaatcgat gggataatcg gggaaaccct ggccgatgtt
     4921 gagcgcgcct tcctcgagcg cacggcgcga cattacagtg aaaatcgtgg tgccgacgtc
     4981 cggcagtttg ctgtcgaagc gcaagatcag ttctccaagg atcgaagacc accggcgagt
     5041 gagcgcaccg caagcccacg ttgtctcaat tcgcgggccg ctgccagact gcgcttgccg
     5101 gaagcgcata ccagtagaaa ttcgcgcgcc gcgaggagca gatgcggatc cgccagcagc
     5161 gcggacatgg ggatgtgccg cgccgcggtc ggctgcgccg cgaattcttc gtcgctgcgg
     5221 atgtcgatca cctccaggcc gtgcattgcg gcttgcgaaa gcgagccgag cgcaatctcg
     5281 ataccgggct cctcgccggc gatctcgcga atatgcgcgc aatcgggggc gcggcattcc
     5341 gcgcggcgcg gcgccttgag tttcaacagc gaaaagttgg tgaaatccac caacagcaac
     5401 tcgcccgcga gttggcccgg caaacgcaac agaatcttca gcgccagcaa ggcctgcagc
     5461 gtgccgaagg tgcccggtac cgggcccaag acccccgcct cggcgcagtt acccactacg
     5521 ccatcggcga tcgcatcggg ccacaggcag cgcaggcacg cgtggcgcat ctccggcttg
     5581 tagacctgca gctgaccctc gtattggtag acgctcgcaa aaattgcggg acgtcgggcg
     5641 atgaccgctg cgtcgttgat caggtacttg ctgcggaaat tgtcggtgca atcgatgact
     5701 acgtcatagg cacgaatcag ctcaagggcg ctgctcgcgt cgagacgcga ggcgtgcaac
     5761 tcgacctgca cgctcggatt gattcgttgg acggccgccc gcgccagttc gactttgggt
     5821 gcgccggcat ccgccaggga aaagatcggc tgacggtgta aattgctggc ctcgagcgcg
     5881 tcggcatcga cgattcccaa acaacccact ccggctcccg ccaggtattg cagcaccggg
     5941 ctgccgaggc cgcccgcacc gacgacgaga acacgcgcgc gccccagggc agcttgtccc
     6001 gatgcaccga tttccttgag caatgtttgc cgtgaatagt cgggctgcat gccgcctact
     6061 tgggattggc gagtgccaca ggctcgtgag tatggcctgg cgtcgccgcg caccgctcgc
     6121 aattcaccca accagaatcg ccgctgcgat agtgctcttt cttccaaatc ggcacgcgat
     6181 gttttacttc gtcgatgata aatcggcagg catcaaaggc ttcgccgcgg tgcgtcgaac
     6241 tgactccaac ccacaccgcc atatcgctca aggacagtgt gccgacgcga tgaatgcaca
     6301 gggcgtgcct cacgggaaag cgtcgcaacg cctcggcgat gatgcgctcg ccttccttca
     6361 cagcgagttc ttcgaatgcc tcgtattcca atcgagacac ctcctgacct tcgttgtgat
     6421 ccctcaccca gccttcaaag gcgacgtagc ctcccgcgcc caaatctaga agctcacgcc
     6481 gcgcccggcc ggtatcgatc gccgtttggc acaagcgaaa ctgcatcagc cacctgccac
     6541 gggggggatg aataccacgg catcgccggc ggtgagccgc cgcgaccaat cgctgaattc
     6601 actattgacc gcgactttca gttgttcgcg tgcgagcgtg aagccgtaac gggcggatag
     6661 ctcgtcgtac aatgcgctcg gcgtcgccgc cgcggtctcg agcgtctcct ccgatcgtcc
     6721 ggcctgctcg cgcatcaagg cgtagtagtg aatgctcagc tgcatggggg cactttacag
     6781 ggaggtgtcg aatgttgagg acgcgtgggc atactcctca ggcgtattga cattgtcgag
     6841 cgcgcgccta tccaacggct cgagcagatg cgcgccatga cgcaacagga attttcgagg
     6901 acattgacct ccgccgtctt gatacgacat cagcgcagcc gccgcttgcg gctcccaaat
     6961 ggcgcacagc ggttccggca agccatcgtg ggcgctgcga aacgccgtcg ccaacgcggc
     7021 ggcatcgcgc tcacgcagca gctgcgacaa cgccgtatcc gacagaaacg gcaggtcgca
     7081 tgccaccacc agccacgcaa cctgggggtg tgcggcgagc gcggaacgaa tgccgacgat
     7141 gggcccttcg ccgggaaatg aatccacgat catgggacgc tgcgctcgag tcggatccgc
     7201 ggtctggctg gcgcgaaccg aggcaaacac cgtcgcgacg tggcggctgg tcagatcgaa
     7261 ggcgcgatcg agctggtgct tgccgccgta gcgcagcgcg gccttgtccc gatgcatgcg
     7321 cgaactcgag ccaccggcca gaatcaggcc atacaccgcg ggagcggccg cagatttcat
     7381 ggcgtcgcct tcaacgcata gctgcttctg ccgccgtcct ttgccagcag ccgcacaccg
     7441 ctgatgacga tgtcatggga cagcgccttg cacatgtcgt atatggtgag cgccgcaata
     7501 ctggcgcccg tgagcgcctc catttctacg ccggtcttgt gatgcacgct caccgtgcag
     7561 cggatcactg cggtgtcatc ctcgagttcg atgctgacgc ggcaattttc caatccgagc
     7621 gggtggcaga acggaatcag ctcatgggtg cgcttcgcgc ccatgacgcc ggcgacgatc
     7681 gccgtatcga agaccggccc tttgggcgaa ctcaagccgc tatcgcgcag cgcagcggcg
     7741 acggctgccg ggaatcgcac ctgcgcttcg gcggtggccg ttcgcttgct cgccttcttg
     7801 tcgctgacat cgaccatggc aggatgccgg tcgctatcta aatgagtaaa ggtcatgggg
     7861 tcagccgccg atgtagttca tctcgatttt cttggtttcc ggcttgacac gccgcaactc
     7921 ctcgcgctgt tcgctgtaac ggtcggtgcg gccgtgccaa acgccgcgga tcaattgcaa
     7981 aagatccgca tcggcggcgc cggcgcgcaa cggcgcacgc aggtcgagac cctgggttgc
     8041 gaacagacag gtatagaatt ttccctccga cgacaggcgc gcgcggctgc acgcgccgca
     8101 gaagggttgc gataccgacg agatgaatcc gacttcaccg gcgccgtcgt caaaacgcca
     8161 gcgctgcgcg acttcgcccg tgtagttctg cgaaatcgga tggatcggcc agcgcgcatg
     8221 cacccgtgcg gcgagttcgc gtgacggcac caccaagtcc gggcgccagt tattgcgatt
     8281 gccgacgtcc atgaactcga tgaagcgcac gatgacgggc gagccgcgga agcgctcgag
     8341 gagatcgagc gctgtgtggt cgttcaaacc gcgctcgatt actgcattga ctttaaccgg
     8401 ggtgaggccg gccgcaatag cgcaatcgat gccgtgcaaa acttgatcga gcgcgtggta
     8461 gccgccgctc atgcgtgcga acacttcctt gtccaaggta tcgagactca ccgtcacgcg
     8521 gcgcaggccg ttggcatgta gatcgacggc gtgttgtccg agaagtacgc cattggtggt
     8581 gagtgcgatg tcgtcgatcc ccggaacatc gctcagatcg ccgatcaaat ccgcaaggtt
     8641 ggtgcgcagc agcggttcgc caccggtgag gcgcagtttg cgtaccccca ggttcgcgaa
     8701 caatcgcgcg agacggacga tctcgtcgaa ggacaaccgc tcctgggact tcaaaaatcg
     8761 atagtgttcg tggtattgct ctttcggcat gcaataaggg cagcgaaaat tgcaccggtc
     8821 catgaccgaa atccgcaagt cgtgcaacgg acggcccagc cgatcgagcg ggtctttcgg
     8881 tccatgaatg tgatgaacgg tcgaactcat gactttgagg ttaccagcgg taaatggtcg
     8941 tgacgaaacc tttggggtat gtattgggg
//
LOCUS       DASWZT010000438         3154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_80882, whole genome shotgun sequence.
ACCESSION   DASWZT010000438 DASWZT010000000
VERSION     DASWZT010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80882"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="VGE92_12475"
     CDS             complement(<1..813)
                     /locus_tag="VGE92_12475"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY1314691.1"
                     /translation="MSNTRVNPLSKSQNGRASLPEKIGKYVIINEVGRGSTGVVYLSH
                     DPYYGRDVAIKVYNVDSSGDENRKRIARKMFLSEAHMVGMLQHPNILPIYDAGEEGGH
                     CYIVTEHVHGARTLAAYCRPDNLLRLDDVVEIMFKCAKALQYAHSRGVIHRDVKPSNI
                     MLTTDSDVRIIDFGIALVADSEISRIEGIAGSPSYMSPEQVQSLELTNRSDLYSLGAV
                     MYELLTGVRPFRAGNLAKLLHQIVYATPAPIHTLRNDVPEEIENVVAVAMQKD"
     gene            complement(902..1891)
                     /gene="thiL"
                     /locus_tag="VGE92_12480"
     CDS             complement(902..1891)
                     /gene="thiL"
                     /locus_tag="VGE92_12480"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEY1314692.1"
                     /translation="MSRASADLGEFELIRRFFQDRQGARAQPGVILGIGDDAALLALP
                     PHTELVAAVDTLVAGRHFPDATAARSIGHRALAVNLSDLAAMGATPAWATLALTMPQV
                     DAVWLEQFAAGFLDLANANGVALVGGDTTRGPLTVSVQIMGHVPCGAALRRSGARAGD
                     LLAVTGTLGDGGAGLAFATATASSQVPLAVQELIQRFDYPTPRVQFGLAARGIASAAM
                     DISDGLVGDLPKLAHASGVAAHVAVESLPLSDALRAAVPYAQARDWALAAGDDYELLL
                     AVPAARHADLAAAAAQLNLRLTVIGAISAGSGVTWSQNGSDFAPGVRAWDHFA"
     gene            complement(1895..2332)
                     /gene="nusB"
                     /locus_tag="VGE92_12485"
     CDS             complement(1895..2332)
                     /gene="nusB"
                     /locus_tag="VGE92_12485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY1314693.1"
                     /translation="MTGKRARSFARRLALQALYQIQINPRPWADTQQQFATDPEAQRV
                     DVDYFRALLAAIAPNREALDARLAHLCEIPPAELDPIEHAVLWLGLYELESCPDLPYR
                     VALAEAVQLAKQFGATDGHKFVNAVLDRAARELRPQEYGVPAA"
     gene            complement(2333..2794)
                     /gene="ribE"
                     /locus_tag="VGE92_12490"
     CDS             complement(2333..2794)
                     /gene="ribE"
                     /locus_tag="VGE92_12490"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005173754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEY1314694.1"
                     /translation="MVEGELQARDLRFALIAARFNEFVVEPLVRGALDALRRHGVGDK
                     QIEIVRVPGAFDIPIVARKLALSRRYEALIALGAVIRGQTPHFDYVAGECAGGIARIA
                     LESGVPIAFGVLTTDTVEQAVDRAGGKAGNKGADAALVAIEMANLLRRLEP"
     gene            complement(2862..>3154)
                     /locus_tag="VGE92_12495"
     CDS             complement(2862..>3154)
                     /locus_tag="VGE92_12495"
                     /inference="COORDINATES: protein motif:HMM:NF013118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314695.1"
                     /translation="PLRAAIRRVASEPSGVVVILRPDETPRHVMDSVRLLDSKRPNTR
                     HAGATVVRTYGIGAQILKDLGMKRMRVLSAPKQLQGLAAYDLEVTSYVDGGE"
BASE COUNT          559 a         1036 c         1007 g          552 t
ORIGIN      
        1 gtccttctgc atcgcgactg ccacgacgtt ctcgatttcc tcgggcacgt cattgcgtag
       61 cgtgtggatc ggcgccggcg tcgcgtatac gatctggtgc agcagcttgg cgagattgcc
      121 ggcgcggaac ggccgcacgc cggtcagcag ctcgtacatc accgcgccga gcgaatacag
      181 gtcggagcgg ttggtcaatt ccagactctg gacctgctcg ggggacatgt agctgggcga
      241 gcccgcgatg ccctcgatgc gcgagatctc cgagtcggcg accagcgcga tgccgaagtc
      301 gatgatgcgc acgtcgctgt cggtcgtgag catgatgttc gagggcttga cgtcacggtg
      361 aatcacgccg cgcgaatgcg cgtactgcag tgccttggcg cacttgaaca tgatctcgac
      421 cacatcgtcg agtcgcagca gattgtccgg ccggcagtac gcggcgagcg tgcgcgcacc
      481 atgcacgtgc tcggtcacga tgtagcagtg gccgccctcc tcgcccgcgt cgtagatcgg
      541 caggatgttc gggtgctgca gcatgccgac catgtgcgcc tcggacaaga acatcttgcg
      601 cgcgatgcgc ttgcgattct catcgccgct cgaatcgacg ttgtacacct tgatggcgac
      661 gtcgcgtccg tagtaaggat catgcgagag atacacgacc ccggtgctgc cgcgtcccac
      721 ctcgttgata atcacatact tgccgatctt ctctggtaac gaggcgcggc cgttctggga
      781 tttactgaga ggatttacgc gagtgttgct caagggtaac cgtgcgctgc agggcgtgtc
      841 tgttcgactg gcccaaagga tactggggcc actggcggta aactgtgatt tgagtcgagg
      901 cttaggcgaa gtgatcccag gcgcgcacgc ctggcgcgaa atcgctgccg ttttgtgacc
      961 aggtcacacc gctgccggcg ctgattgcac cgatgacggt caatcttaag ttcaattggg
     1021 ccgctgcggc tgcaagatcg gcatggcgcg cggccggcac ggcgagcagc aattcgtagt
     1081 catcgcctgc ggccagcgcc caatcgcgtg cctgagcgta cggcaccgcg gcgcgcagcg
     1141 catcggacag cggcaaactc tcgacggcga catgcgcagc gacgccgctc gcgtgcgcaa
     1201 gtttcggcag gtcgccgacc agtccgtcgg aaatatccat cgccgcgctt gcgatgcctc
     1261 gcgccgccag accgaactgc acgcgcggcg tcgggtaatc gaaacgttgt attagttcct
     1321 ggacggcgag cggcacttgc gacgacgcgg tcgcggtagc gaacgcaaga cccgcgccgc
     1381 cgtcaccgag agtgccggtg acggccaaca gatctccggc gcgcgcgccc gagcggcgca
     1441 gcgcggcgcc gcacggcacg tgtcccatta tttgcacgct tacagtcaga ggaccgcgcg
     1501 tggtatctcc gccgaccagg gcgacaccgt tcgcattcgc tagatccagg aaccccgccg
     1561 caaattgctc cagccacacg gcgtcgacct gcggcatcgt cagcgccaag gtcgcccagg
     1621 cgggcgtcgc acccatggcg gctagatcgc tcaggttgac cgccagggcg cgatggccga
     1681 tcgaacgcgc agcggtggcg tcggggaaat gacgcccggc aaccagggta tcgaccgccg
     1741 ccaccaattc tgtgtgcggc ggcaaggcga gcagcgcggc atcatcgccg atgccgagaa
     1801 tgacgcccgg ctgggcgcgt gctccctggc ggtcctgaaa gaagcggcga atcagctcga
     1861 actcgccgag atcggcactc gcgcggctca tcggctaggc ggcgggcacg ccgtactcct
     1921 gcggccgcaa ttctcgggcg gcccgatcga gcacggcgtt gacgaatttg tgcccatcgg
     1981 tcgcgccgaa ctgcttggcg agctgcacgg cctcggccag cgccacgcga tatggtaagt
     2041 cggggcacga ttccagctcg tacagcccga gccacaaaac ggcatgctcg atcggatcga
     2101 gttcggcggg cggaatttcg caaagatgcg cgagccgcgc atccaacgcc tcgcggttcg
     2161 gcgcgatcgc ggcaagcaac gcccggaagt aatccacatc gacgcgctgc gcctccgggt
     2221 ccgtcgcaaa ttgctgctgc gtgtcggccc aggggcgcgg attgatctgg atctgataga
     2281 gcgcctgcaa tgccaatcga cgcgcgaagc tccgggcacg cttgccggtc acctacggtt
     2341 ccaagcgacg caacagattc gccatttcga tcgcaaccag cgcggcgtcg gcccctttgt
     2401 tgccggcctt gcccccggcg cgatccaccg cctgttccac cgtgtcggtg gtcagcaccc
     2461 cgaacgcaat cggcacgccg gattcgagcg cgatgcgtgc gatgcctccg gcacattcgc
     2521 cggcaacgta atcaaaatgc ggcgtttgcc cgcggatcac ggcgccaaga gcgatgagcg
     2581 cttcatagcg gcgcgacaac gccaacttgc gcgccacgat cggaatatcg aacgcacccg
     2641 gtacgcgcac gatttctatt tgcttgtcgc ccacaccatg gcgccgcaac gcatcgaggg
     2701 cgccgcgcac cagcggttcg accacaaatt cgttgaagcg cgccgcgatc agcgcgaaac
     2761 gcaaatcgcg cgcctgcaat tcgccttcga ctatcttcgg ctgatcggtt ggcacaaatc
     2821 gttcctttga agtgtggcgc gaagtatagc cttcggcccg gttattcgcc gccgtcgacg
     2881 tagcttgtga cctcgagatc gtaggccgcg agcccctgca gctgtttggg ggcgctcaac
     2941 acgcgcatgc gcttcattcc gagatccttg agaatctgcg cgccgatccc gtaggttcgc
     3001 accaccgtgg ctccggcgtg gcgcgtgttc gggcgcttgc tgtccagcag gcgcacgctg
     3061 tccatcacat gacgcggcgt ctcgtcgggc cgcaatatca cgaccacgcc cgagggctcg
     3121 ctcgcaacgc ggcgaatcgc cgcgcgcaac ggcc
//
LOCUS       DASWZT010000439         3733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_37339, whole genome shotgun sequence.
ACCESSION   DASWZT010000439 DASWZT010000000
VERSION     DASWZT010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37339"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2257)
                     /locus_tag="VGE92_12500"
     CDS             complement(<1..2257)
                     /locus_tag="VGE92_12500"
                     /inference="COORDINATES: protein motif:HMM:NF003619.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEY1314696.1"
                     /translation="MVQQTTARTLDADTAQRIDAYWRAANYLSVGQIYLCDNPLLKEP
                     LTLAHVKRMLLGHWGTTPGQNFIYTHLNRVIKKYDLDMIYMSGPGHGGPAVVGGTYLE
                     GTYSEIYSNISQDEAGLKRLFTQFSFPGGIPSHASAECPGSIHEGGELGYSLSHAFGA
                     VFDNPELVVACVVGDGEAETGPLATSWHSNKFLNPASDGAVLPILHLNGYKIANPTLL
                     ARISRDELQQLLRGYGWTPHFVEGHEPLPMHEAMATTLDAVIEEIKRIQADARLHGNT
                     ERPYWPMIVLASPKGWTGPKTVDGKANEGTFRSHQVPLSDPAKNPEHLRQLESWLRSY
                     RPRELFDDNGRLRPELAALAPSGERRMGANPHANGGVLLRDLRLPDYTVYAVQVDRPG
                     VKGIGDTHVLGRFLRDVVKLNAAAGNFRLFGPDETLSNGLEAVFEATNRQWLAATADN
                     DEFLARSGRVMEMLSEHQCEGWLEGYLLTGRHGLFNCYEAFIHIVDSMFNQHAKWLKV
                     TAGLAWRRKIASLNILLSSHVWRQDHNGFTHQDPGFLDHVVNKKAEIVRVYLPPDANC
                     LLSVMDHCLRSRHYVNVVVAGKHPAPQWLSIDAAVKHCSEGIGIWQWASNDQRGEPDL
                     VMACAGDVPTLETLAAVSILREHFPDLRIRMVNVVDLMKLQPKSEHPHGLEDSEFDSL
                     FTKDKPIIFAFHGYPWLIHRLTYRRHNHENLHVRGYKEEGTTTTPFDMCVRNELSRYH
                     LAMMVLERVQRL"
     gene            complement(2261..3427)
                     /locus_tag="VGE92_12505"
     CDS             complement(2261..3427)
                     /locus_tag="VGE92_12505"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEY1314697.1"
                     /translation="MPNILTLNGGSSSIRFAIFAAAQPLKRLLQGKMERIGSADATLS
                     FRREGEDAPTVLAATGSLLEWLESQPLFESVRGVGHRVVHGMLRRAPELVTAQLLDEL
                     ADIIPFDPEHLPRELELIRSLQRRYPDLPQVACFDTAFHRSMPLVATQLPIPRRYAAR
                     GVQRYGFHGLSYTYLMQELARLGDPAAQRGRVILAHLGSGASLAAVRDGSSVDTTMGF
                     TPAAGLVMGTRSGDLDPGLPGYLAAMDHMSAAQFQSMVNHESGLLGMSETSADVRDLL
                     ARETQDSRAAEAIALFCYQAKKWIGAFAAALGGLDTLVFAGGIGENAAAVRERICAGL
                     EFLGIDIDADANRRHAARISAAHGSVTVRIIPTDEESVIARLSMRLLKLSPEEG"
     gene            3540..>3733
                     /locus_tag="VGE92_12510"
     CDS             3540..>3733
                     /locus_tag="VGE92_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314698.1"
                     /translation="MSHFHAVVWIDHQKATVWQFSPSEQQSTVVHAHDPHQRVHSRKS
                     THGGHKSPADRHFFDDTAAAL"
BASE COUNT          734 a         1219 c         1093 g          687 t
ORIGIN      
        1 ccaaccgttg cacgcgctcg agcaccatca tcgcgagatg gtagcgcgag agctcgttgc
       61 gcacgcacat gtcgaagggc gtcgtcgtcg tgccctcttc cttgtagccg cgcacatgca
      121 aattctcatg gttgtgccgg cggtacgtca ggcggtgaat caaccaagga tagccgtgaa
      181 aggcgaagat gatcggcttg tccttggtaa acaatgaatc gaactcgctg tcctcgaggc
      241 catgcggatg ttccgacttg ggctgcagct tcatgagatc gacgacgttg accatgcgga
      301 tcctcagatc cggaaagtgc tcgcgcaaga tcgacacggc cgcgagcgtc tcgagcgtcg
      361 gcacatcgcc cgcgcaggcc atcaccaagt ccggttcgcc gcgctgatcg ttgctcgccc
      421 actgccagat gccgatgccc tcgctgcaat gcttgacggc cgcgtcgatc gacaaccact
      481 gcggcgccgg atgcttgccc gccacgacga cgttgacgta atgacggctg cgcaggcagt
      541 gatccatcac cgacaacagg caattcgcgt cgggcggcag gtagactcga acgatttccg
      601 ccttcttgtt gaccacatga tcgagaaacc cgggatcctg atgcgtgaag ccgttgtgat
      661 cctgccgcca gacatgcgaa cttagcagga tattgagcga ggcgatcttg cggcgccatg
      721 ccaaacccgc cgtcaccttc aaccatttcg catgctgatt gaacatcgaa tcgacgatgt
      781 gaatgaacgc ttcgtagcaa ttgaacagac cgtgccgccc ggtcaacagg tagccttcga
      841 gccacccctc gcactggtgc tcgctcaaca tctccatcac ccgaccactg cgcgccaaga
      901 actcgtcgtt gtcggcggtc gcggccaacc attgccgatt ggtcgcttcg aacaccgctt
      961 ccaagccgtt cgatagcgtc tcgtccggtc caaacagccg aaaattaccc gccgcggcgt
     1021 tcagcttgac cacgtcgcgc aaaaaccgtc cgagtacatg cgtatcgccg atacccttca
     1081 cgccgggtcg gtccacctgc acggcgtaga cggtgtagtc cggcagcctg aggtctcgca
     1141 acagcacgcc gccgttcgca tgcggattcg cacccatgcg ccgctcgccg ctcggcgcca
     1201 gcgcagcgag ctcgggccgc aaccgcccgt tgtcatcgaa gagttcgcga ggacgatagc
     1261 tgcgcagcca gctctccagt tggcgcagat gctccgggtt cttggcggga tccgacagcg
     1321 gtacttgatg cgacctgaat gttccttcgt tcgccttgcc atcgaccgtc ttcggtcccg
     1381 tccaaccttt cggcgaggcc aatacgatca tcggccaata cggccgctcg gtgttgccgt
     1441 gcaggcgggc atcggcttga atgcgtttta tttcctcgat gaccgcatcg agcgtcgtcg
     1501 ccatcgcttc gtgcatcggc aaaggctcgt gaccttcgac aaagtgcggc gtccatccgt
     1561 aaccgcgcag cagctgctgc aattcatcgc gtgaaatgcg cgccaacaga gtcggattgg
     1621 cgatcttgta gccgttcaag tgcaatatcg gcaataccgc cccgtcgctc gccggattca
     1681 ggaatttatt cgaatgccag gaggtcgcga gcggcccggt ttcggcctcg ccatcgccga
     1741 ccacgcaggc caccaccagc tcgggattgt cgaacacggc gccgaacgcg tggctcaacg
     1801 agtagccgag ttccccgccc tcgtgaatcg atccgggaca ctccgccgag gcgtggctcg
     1861 gtatgccgcc cgggaacgag aactgcgtga acaagcgctt caagccggcc tcatcctggc
     1921 tgatgttcga ataaatctcg ctataggtac cttccaggta ggtgccgccg accaccgccg
     1981 gaccgccatg ccccggcccc gacatataga tcatgtccag atcgtatttc ttgatgacgc
     2041 gattcaagtg ggtataaatg aagttctggc cgggcgtcgt tccccagtga ccgagcaaca
     2101 tgcgcttcac gtgcgcgagc gtgagcggct ccttcaacaa agggttgtcg cacaaataaa
     2161 tttgcccgac ggacagataa ttcgccgccc gccaataggc atcgattcgc tgggcggtgt
     2221 cggcatcgag tgttcgcgcg gttgtctgtt gcaccatgag tcaaccctcc tcgggcgata
     2281 gtttaagcag gcgcatgctc aagcgggcga tcacggattc ctcgtcggtg gggatgattc
     2341 gcaccgtgac ggatccgtgc gcggcggata tgcgcgccgc atggcggcga ttcgcatccg
     2401 cgtcgatgtc gattccgagg aactcgaggc cggcgcagat acgctcgcgc accgcggccg
     2461 cgttctcgcc gatgccaccc gcaaaaacca gcgtgtcgag gccgccgagc gcagcggcga
     2521 acgccccgat ccacttcttt gcttgatagc aaaacagcgc gatcgcctcc gccgcgcgcg
     2581 agtcctgcgt ctcccgcgcc aacaagtcac gcacatccgc gctggtttcc gacatgccga
     2641 gcaaccccga ttcgtggttg accatgcttt gaaattgtgc ggcgctcata tggtccatcg
     2701 ccgcgagata acccggaagc ccgggatcga gatcaccgct tcgcgtaccc atcaccaagc
     2761 ccgccgcggg tgtgaaaccc atggtggtat cgacgctgct gccgtcgcgc accgcggcga
     2821 ggctcgcgcc gctgcccagg tgcgcgagaa tgacgcgccc gcgttgcgcc gcgggatcgc
     2881 ccagccgcgc aagttcctgc atcaagtacg tgtaggataa accgtgaaag ccatagcgct
     2941 gaaccccgcg cgccgcatag cggcgcggaa tgggtaattg cgtcgcaacc agcggcatgc
     3001 tgcgatgaaa ggccgtatcg aagcacgcca cttgcggcag atccgggtag cgccgctgca
     3061 agctgcgaat cagttcgagc tcgcggggca ggtgttccgg atcgaaggga atgatgtccg
     3121 cgagctcgtc cagcaactgc gccgtgacga gttcgggagc tcgccgcaac atgccgtgca
     3181 ccaccctgtg gccgacgccc cgcacgctct cgaacagcgg ctgcgactcc agccattcga
     3241 gcagggaacc ggtcgcggca aggaccgtcg gcgcatcctc gccctcgcgg cggaagctta
     3301 aagttgcgtc ggcactgccg atgcgctcca tttttccctg caacagccgt ttgagcggct
     3361 gcgctgccgc gaagattgcg aagcgaatgc tcgaggagcc gccattgagg gtcaagatat
     3421 tcggcatcga ggcacctgag tagtacaaac gacaaccgcg cgcgtaggcg cgcagctccg
     3481 caagggctac aataactcaa cgcgcgacgc tagagcttaa gttcgcaggg gaaacaacca
     3541 tgtcacattt ccatgccgta gtctggatcg atcatcagaa ggcgacggtt tggcaattct
     3601 cgccgagcga gcagcagagc accgtggtcc atgcgcacga cccccatcag cgcgtccaca
     3661 gccgcaagag cactcacggc ggccacaagt ctcccgccga tcggcacttc ttcgacgaca
     3721 ccgccgccgc gct
//
LOCUS       DASWZT010000440         1948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_85626, whole genome shotgun sequence.
ACCESSION   DASWZT010000440 DASWZT010000000
VERSION     DASWZT010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85626"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VGE92_12515"
     CDS             <1..451
                     /locus_tag="VGE92_12515"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY1314699.1"
                     /translation="ITTILNQQHESSRELRVPFSILMADLDHFKKINDHHGHITGDTV
                     LKAVTGVLQAAMRVDDELGRYGGEEFIAILPNTNAADAEELAQRLNESVAELRVDTPT
                     GSIGCTVSIGVAAWTPTRLESVQALVNRADAALLSAKQCGRNRVRAS"
     gene            complement(448..>1948)
                     /gene="rfaE1"
                     /locus_tag="VGE92_12520"
     CDS             complement(448..>1948)
                     /gene="rfaE1"
                     /locus_tag="VGE92_12520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HEY1314700.1"
                     /translation="NNASAWFESPVRLDSLFGVLSKFHAARILVLGDVMLDRFIYGSV
                     ERISPEAPIPVVNVQRFMDMPGGAANVARNIAALGGRAVLLGVVGDDAWAQDLRTQLA
                     LSPSIESRLVTDTARVTSVKTRYVADGQQVMRADRESRMPLPEPVAQRLLEEFSAAMR
                     DVDVIILSDYAKGVLSEPVTAAAISLARGAGKTIIVDPKAKDLSKYRGSSLLTPNRIE
                     LQTACGSECSSDEQVVAGARRLLEQNICDVMIVTRGKDGMSLIKADGFAAHLPTAARQ
                     VFDVSGAGDTVVATLSLGLAAGAEIAGAAALANVAAGIVVGKRGTAVVTTGEIVAALR
                     PFDGRTDPQKIFALDSVMQLARAWREQGLKIAFTNGCFDLLHPGHISLLDQARHTADR
                     LIVGLNADVSIRRLKGPNRPVQSEVARATVLAAVKSVDAVVIFSEDTPIHLIETLEPD
                     VLVKGADYTLGTVVGADLVIGRGGKVVLAELLSGHSTTDTVQRVANTNH"
BASE COUNT          383 a          612 c          583 g          370 t
ORIGIN      
        1 catcacgacg atccttaatc aacagcatga gagttctcgg gaactacgcg tcccattctc
       61 aattcttatg gccgacctgg atcactttaa aaaaattaac gaccaccatg gtcacatcac
      121 tggggacaca gtcctcaaag ccgtcaccgg tgtcctgcag gcggccatgc gggtcgacga
      181 tgagctaggg cggtacggcg gcgaagaatt tattgcaatt ttgccgaaca ccaatgcggc
      241 ggatgccgag gagttggctc agcggctcaa tgagtccgtt gccgaactga gggtggacac
      301 gcccacggga tcgattggat gtaccgtcag cattggcgtc gctgcgtgga cgcccacccg
      361 gttggagtcc gtccaggcgc tcgtgaaccg cgctgatgcg gcgctacttt cggcgaaaca
      421 atgcggacgc aaccgcgtgc gtgcgtctta atgattcgtg ttcgcgacgc gttgcaccgt
      481 gtcggtggta ctgtgcccgg acaacagctc ggcgagcacc accttgccgc cgcggccgat
      541 cacgaggtcg gcgccgacga cggtgcccaa agtatagtcg gcgcccttga ccaacacgtc
      601 cggctcgagc gtctcgatca ggtggatggg agtatcttcc gagaagatga cgacggcatc
      661 gaccgacttg accgctgcca acaccgttgc gcgcgccacc tcgctctgca cgggcctgtt
      721 cggccctttc aatcgccgaa ttgagacatc cgcattgagt cccacgatca accggtccgc
      781 ggtgtggcgc gcctgatcga ggagagagat atgtccggga tgcaataaat cgaaacagcc
      841 gttggtgaat gcgattttca gtccctgttc cctccacgca cgggccagtt gcatcacact
      901 gtccagggca aagatttttt gcgggtcggt gcgcccgtca aatggacgca gcgcagcgac
      961 gatttctcca gttgttacga ccgccgtacc ccgtttcccg acgacgatgc cggcggcgac
     1021 attcgccagg gccgccgcgc cggcaatctc ggcgcccgcg gcaagaccca gtgagagcgt
     1081 tgcaaccacg gtatcgccgg cgcccgacac gtcaaacacc tgccgggctg cggtcggcaa
     1141 atgcgcggca aatccatcgg ccttgatcag ggacatcccg tctttgccgc gcgtgacgat
     1201 catgacgtcg caaatgttct gctccaggag gcggcgcgcc ccggcaacca cttgctcgtc
     1261 ggaactacat tccgaaccgc aggcagtctg cagctcgatt cgatttggag tgagcaatga
     1321 ggagccgcgg tacttcgaga gatccttcgc tttggggtca acgatgatgg ttttcccggc
     1381 gccgcgcgcc aggctgatgg ctgcggccgt gaccggctcg gacaacacgc ccttcgcata
     1441 gtccgacaat ataattacat ccacgtcgcg catcgcggcc gagaattcct cgagcagacg
     1501 ctgcgcgacc ggctccggga gcggcatccg gctttcgcga tctgcgcgca tgacttgctg
     1561 tccgtctgcg acgtagcggg tcttgaccga ggtgacgcgc gcggtatccg tgacgaggcg
     1621 cgactcgatg cttggcgata gcgctaattg agtgcgtagg tcctgcgccc atgcatcgtc
     1681 gcccacaacg ccgagcagta ccgcgcgccc acccagggcc gcgatgttgc gcgccacgtt
     1741 ggcggccccg cccggcatgt ccataaaccg ctggacgttg accaccggta tgggcgcttc
     1801 cggtgatatg cgttcaaccg atccatagat gaatcgatcc agcatcacat cgccaagcac
     1861 gaggatccgc gctgcgtgaa acttgctcaa tacgccgaac agtgaatcta ggcgcacagg
     1921 cgattcaaac caggccgagg cgttgttc
//
LOCUS       DASWZT010000441         2755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_32699, whole genome shotgun sequence.
ACCESSION   DASWZT010000441 DASWZT010000000
VERSION     DASWZT010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32699"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VGE92_12525"
     CDS             <1..286
                     /locus_tag="VGE92_12525"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HEY1314701.1"
                     /translation="IDLAAADGAQPSEVLLVSYERSAVSAIGRGENAGRTLAEFNIVR
                     SIRSLGAYDGRLQQYRAAVKTLPSDATDVAVLVQSVGQSAIIGAAAHSLR"
     gene            323..1477
                     /locus_tag="VGE92_12530"
     CDS             323..1477
                     /locus_tag="VGE92_12530"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY1314702.1"
                     /translation="MSLASLWCLDPSVTYLNHGSFGACPEAVLAVQTALRRELEREPV
                     DFLVNSLPARLAAARAELCAFLGADSADLVFVPNATTGVNAILRSLSFTPGDELLLTN
                     HTYAACRNTADFVCQRSGARVVVAQLPFPCPHEDEIVASVLSCVTPRTRLALLDHVTS
                     PTALVLPVARLVSGLESRGVDTLVDGAHAAGMVALELSTLGAAYYTGNAHKWLCAPKG
                     AAFLHVRRDRQAQLHPNVISHGYGAGFHAEFDWTGTFDPTAWLCVPEALRYIEGLKPG
                     GWPEVMAANRALALSARSLLLDRLGLEAPCPDTMIGSMASVPLPPAAAGSPAQRFDAK
                     ALHAWFRERGVETWLYPLPVPLLRISAQLYNDLDQFERLANLLEEALRGS"
     gene            1467..>2755
                     /locus_tag="VGE92_12535"
     CDS             1467..>2755
                     /locus_tag="VGE92_12535"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY1314703.1"
                     /translation="MAAEQEQSQIRARLSTFDTAMVVFSLVVGIGIFRTPTIVAGAAG
                     GTTLFFVAWIAAGIISLIGALTFAEIGSRYPRAGGYYRVVADCYGPTPAFMLNWSQTL
                     MQGAGAAAVAFIGADYLMPVLLPPSWRTAHASLVLACATMLILLIFNYRGVKPGAHVQ
                     NFLSILKIAMIVGLAVLALLLAPHAHVLEPRSQAPLAPRLLSALIPCFYAYGGYQLTM
                     NLGADMQQARRRFPLAITAGMITVVTLYLLLNFSYQRVLGIAGIAGSKLVAAALSRAT
                     FGPSGEMLVSIAVFLSAAGFVNATIIQMPRSFYAMAQDGVLPRAFLRVNPDTQVQEVG
                     LLCFGATMLLPAFLLGSFEKLLQYVIFTDMLTLAVVASTLFVLRRRRTGEGGFSMPGY
                     PWLPAGYMVCLLGVAGRVFTVEPRLALAGIVILATGWP"
BASE COUNT          396 a          907 c          880 g          572 t
ORIGIN      
        1 gatcgatctg gctgctgcgg atggcgcaca gccaagcgag gtgctgctgg tgtcctacga
       61 gcgtagtgca gtatcggcga tcggccgcgg agagaacgcc ggtcggacac tcgcagaatt
      121 taacatcgtc cgttccattc gcagtctcgg tgcctacgac ggccggttgc agcagtatcg
      181 cgccgccgtc aaaacactcc cgagcgatgc caccgatgta gccgtcctgg tgcagtccgt
      241 cggtcagagc gccatcatcg gcgcggcggc acacagcctg cgttgatata atccgtagcc
      301 gtcccttctc gaggagccga atttgagctt ggcctcgctt tggtgcctgg acccgtcggt
      361 gacctatctc aaccacggct ccttcggcgc ctgcccggag gcagtgctcg ccgtacagac
      421 ggccttgcgc cgcgagttgg agcgcgagcc ggtggatttc ctggtgaatt ccctgcccgc
      481 ccgcctcgct gcggcgcggg ccgaattgtg cgcctttctc ggagccgatt cggccgatct
      541 tgtgttcgtg ccgaacgcaa ccaccggcgt gaacgcgatt cttcgctcac tatcgtttac
      601 acccggcgat gaattgctcc tcaccaatca cacgtacgcg gcgtgccgca acaccgccga
      661 tttcgtgtgc caacgaagcg gcgcgcgggt ggtggtggcg cagctgcctt tcccgtgccc
      721 tcatgaagac gagatcgtcg cctcggtgct gagttgcgtg acgccgcgca cgcgtttggc
      781 actgctcgac cacgtaacga gtccaacggc actggtcctg cctgtggcgc gcctggtcag
      841 cggcctcgag tcgcgcggcg tcgatacttt ggtcgatggc gcgcatgccg ccggcatggt
      901 ggcgctcgaa ttatcgacgc tgggagcggc ttactacacc ggcaacgccc acaagtggct
      961 gtgcgcgccg aagggcgccg cgttcctgca tgtgcgccgc gaccgccaag cgcagctgca
     1021 tccgaatgtg atcagccatg gctacggcgc gggatttcac gccgagttcg attggaccgg
     1081 caccttcgat ccgacggcgt ggctgtgcgt cccagaggcg ctgcgctaca tcgagggcct
     1141 caagcccggc ggctggcccg aggtcatggc cgcgaatcgc gccttggcgc tgtcggcacg
     1201 ctccctgctc ttggaccgct tggggttgga ggctccgtgc cccgacacca tgatcggatc
     1261 catggcctcc gtgccgctgc cgccggcagc tgccggttca ccggcgcagc gcttcgacgc
     1321 gaaggctctg cacgcctggt tccgtgaacg cggggtcgaa acgtggctgt atcccttgcc
     1381 cgtgccgctg ctgcgcattt ccgcgcagct ttacaacgac ctcgatcaat tcgagcggct
     1441 tgcgaacctg ctcgaagaag cgctgcgtgg cagctgagca agaacagagc cagattcgag
     1501 cgcggctttc gacgttcgac acggcgatgg tcgtgtttag cctggtggtg ggcattggga
     1561 tttttcgtac gcccacgatc gtcgcgggcg cggccggcgg cacgacgcta tttttcgtcg
     1621 cgtggattgc ggccggaatc atcagcctga ttggcgcgct gaccttcgcg gaaatcggct
     1681 cgcgctaccc gcgcgccggc ggctactatc gcgtcgtggc cgactgctac ggcccgacgc
     1741 cggcgttcat gctcaattgg tcgcaaacgc tgatgcaggg cgccggcgcg gcggcggtgg
     1801 cgttcatcgg tgccgactat ctgatgccgg tgttgctgcc gccatcttgg cgcactgcgc
     1861 atgcctcctt ggtgctcgcg tgcgcgacca tgctcatcct gctgattttc aactaccgcg
     1921 gggtgaagcc gggcgcgcac gtgcagaatt ttctgtcgat cttgaagatt gccatgatcg
     1981 ttggcctcgc cgttctggcg ctgctgctcg caccgcacgc gcacgtgctc gaacctcgat
     2041 cgcaagcccc gctcgcgccg cgcttgttgt cggcgctcat cccctgtttc tacgcctacg
     2101 ggggttacca attgaccatg aatctcggtg ccgacatgca gcaggcgcgg cgccgctttc
     2161 cgctcgccat caccgccggc atgatcaccg tcgtaaccct gtacctgctg ttgaattttt
     2221 cctaccagcg cgtgttgggc atagccggca tcgccggctc gaagctcgtg gccgcggcgc
     2281 tgtcgcgcgc aacattcgga ccctccggtg aaatgctggt ctcgatcgcg gtatttctgt
     2341 ccgccgccgg cttcgtcaat gccaccatca ttcagatgcc gcgcagcttc tatgccatgg
     2401 cgcaagacgg cgttctgccg cgcgcgttcc tgcgcgtgaa tcccgacaca caggtgcagg
     2461 aggtcgggct gttgtgcttc ggggcgacga tgctgctgcc tgcgttcctg ctgggctcat
     2521 ttgagaagct gctgcagtac gtcatcttca ccgatatgtt gacgctcgcg gtcgtggcct
     2581 cgacgctgtt cgtgctgcgg cgccggcgca ccggcgaagg cggattttcg atgcctggct
     2641 atccttggtt gcccgccggc tacatggtgt gcctgctcgg cgtggcgggg cgcgtcttca
     2701 cggtggaacc gagactcgcg ctcgcgggaa tcgtcatcct cgcgaccggc tggcc
//
LOCUS       DASWZT010000442         2852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_42391, whole genome shotgun sequence.
ACCESSION   DASWZT010000442 DASWZT010000000
VERSION     DASWZT010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42391"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1304
                     /locus_tag="VGE92_12540"
     CDS             <1..1304
                     /locus_tag="VGE92_12540"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY1314704.1"
                     /translation="VARMIMLGLKFTGEVPFREVYVHGLIRDHDGQKMSKSKGNVIDP
                     LDIVDGISLEALLAKRTSGLMQPQMKSAIEKATRKQFPLGIPAFGTDALRLCFARLAT
                     QSRDLRFDMSRVEEYRNFCNKLWNAARYVLLNVENADAVAAGAEYSLADRWIQSRLAA
                     MLARVESGFADYRLDVAANALYEFTWHEFCDWYLELSKAVLQSDSAGSAVQRGARFTL
                     INTLEVLLRALHPLAPFISEEIWQRVRLPAGVSGDTIMRCSFPGAADIKVDHQAEQEM
                     RWVIQFIEGVRQIRGELDIAPSRKLEVLLQNAAPRDTEYLGRNLASLMRVAGIDEPRV
                     LKSAQAAPISAVAFVGTLEILVPMAGLIDPGAELDRLAKQRRKAAIDLQKMEAKLGNA
                     EFAKNAPAAVVAKDRQRLAELRTEIGQLEAQIARVSKLKNQ"
     gene            1301..2212
                     /locus_tag="VGE92_12545"
     CDS             1301..2212
                     /locus_tag="VGE92_12545"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY1314705.1"
                     /translation="MSTAQPKIDAILAQINRVILGKDAQIRLCLTCLLARGHLLIEDI
                     PGVGKTTLAHAIARTLGLSFQRVQFTSDLLPADVLGVSIFDRETGKFRFQRGPIFAQL
                     VLADEINRASPKAQSALLEAMEEHQVTLDGQSMALPEPFFVIATQNPQDQVGTFPLPE
                     SQLDRFLMRVHLGYPAPAAERELLNGVDRRDLVNGLSTVASLEDLKRLQQRALEIHVA
                     PALLDYVQALVAHTRHSPGLNAGLSPRAAIGLLRAARAFALIAGRDAVIPEHVQAVFG
                     AVVEHRVRQRDSGPSTVNRLLEAVPIP"
     gene            2217..>2852
                     /locus_tag="VGE92_12550"
     CDS             2217..>2852
                     /locus_tag="VGE92_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314706.1"
                     /translation="MATLRQAAIGKLRAWAARRHGRDSLPIKIHRRRVYILPTRFGIM
                     LALVLVAMLVAGLNYNSNLGLAFAFLLTSIALVTMHHCNRNMLGLTVDVSTEADAFAG
                     RDANFEFVLRNESKVDRRDVEIRCLTGAAVCSVLARSSESARVAIPVAQRGVLRIAQF
                     ELRTRYPFGWFHAWTYVQGSLTVYVAPTPAGTRTLPPAAGAGSVAARSDVRG"
BASE COUNT          503 a          910 c          912 g          527 t
ORIGIN      
        1 gggtcgcgcg catgatcatg ctgggcctga agttcacggg ggaagtacca tttcgcgaag
       61 tctacgtgca tggcctgatc cgcgaccatg acggccagaa gatgtccaag tcgaagggta
      121 acgtcatcga tcccctggac atcgtcgatg gcatctcgct cgaggcgctg cttgccaagc
      181 gcaccagcgg gttgatgcag ccgcagatga aatcggccat cgagaaagcc acccgcaagc
      241 aatttccgct cggaatcccg gccttcggca cggatgcact gcgcctgtgt tttgcgcggc
      301 tcgcgacgca aagccgcgac ttgcgcttcg acatgagccg cgtcgaggaa taccgcaatt
      361 tctgcaataa gctgtggaac gcagcacgct acgtgctgct caatgtcgag aatgcggacg
      421 ccgttgccgc aggcgccgag tacagcttgg cggatcgctg gattcagtcg cgtctcgcgg
      481 ctatgctcgc gcgcgtcgaa tccggctttg cggactaccg cctcgatgtc gccgccaacg
      541 cgctgtacga gtttacctgg cacgaattct gcgattggta tctggagctg tccaaggcgg
      601 tgctgcaatc ggactccgcc ggcagcgcgg tgcaacgcgg tgcgcgattc acgctgatca
      661 ataccctcga agtgctgctg cgcgcgctgc atccgctggc gcccttcatc agcgaggaga
      721 tctggcagcg ggtgcgcctg cctgcgggcg tgtccggcga caccatcatg cgttgcagtt
      781 tcccgggcgc ggcggacatc aaggtcgacc accaagcgga acaggaaatg cgctgggtga
      841 tccagttcat cgagggagtg cggcaaatcc gcggcgaact cgacatcgcg ccgagccgca
      901 agctcgaggt gctgctgcaa aatgccgcgc cccgcgacac cgagtatctc gggcgcaatc
      961 tggcctcttt gatgcgggtt gccgggatcg atgagccgcg cgtcctaaag agcgctcaag
     1021 ccgcgccgat ctcggccgta gcattcgtag ggacactcga gattttggtg cccatggccg
     1081 gcctgatcga ccccggcgcc gagctcgacc gcttggcgaa acagcggcgc aaggccgcca
     1141 tcgatttgca gaagatggag gccaagttgg gcaacgccga attcgccaag aatgcaccgg
     1201 ccgcggtggt cgccaaggac cggcagcggc tggcggaact gcgtaccgag atcggtcaat
     1261 tggaggccca gatcgcgcgg gtgtccaagc tcaagaacca atgagcacgg cgcagcccaa
     1321 gatcgacgcc attctcgccc agatcaaccg cgtgatcttg gggaaggatg cgcaaatccg
     1381 cctgtgtttg acctgcctgc tggcgcgcgg ccacctgttg atcgaggaca tacccggcgt
     1441 gggcaaaacc acactggcgc atgcgatcgc caggaccttg ggattgtcct ttcaacgcgt
     1501 gcagttcacc agcgacctgt tgcccgcgga cgtgctgggc gtgtcgatct tcgaccgcga
     1561 gaccgggaaa ttcagatttc aacgcggccc gatcttcgcg cagctcgtgc tggcggacga
     1621 aatcaaccgc gcttcaccga aagctcaaag cgccttgctc gaagccatgg aagagcatca
     1681 agtcacgctc gacggtcagt cgatggcgct gcccgaacct ttcttcgtga tcgcgacgca
     1741 aaatccgcaa gaccaggtcg gcacctttcc gttgccggag tcgcaattgg accggttctt
     1801 gatgcgcgtc cacttggggt atccggcgcc cgccgccgag cgtgaacttc tgaacggcgt
     1861 cgatcgacgc gatctcgtga atggactctc gacggttgcg agcctcgagg atctcaagcg
     1921 cttgcagcag cgagcgctcg agatccacgt ggcgccggcc ctgctcgact atgtgcaggc
     1981 cctcgtggcc cacacccgcc actcgcccgg cttgaacgcc ggactatcgc cgcgggcagc
     2041 gatcgggcta ttgcgtgccg cccgggcctt cgccctcatt gccggccgcg acgccgtcat
     2101 tcccgagcac gtgcaggcgg tgttcggcgc cgtggtggag catcgggtgc ggcagcgcga
     2161 cagcgggccg agcacggtca accggctgct cgaggccgtt cccatcccgt aggcgcatgg
     2221 ccactctacg gcaggcggcg atcggcaaac tgcgggcctg ggcggcgcgg cgccacggcc
     2281 gcgattcgtt gccgattaaa attcaccgcc gccgtgtcta cattctcccg acacgcttcg
     2341 gcatcatgct ggcgctggtg ctggttgcaa tgctggttgc cgggctcaac tacaacagca
     2401 atctggggtt ggcctttgct tttctcctga ccagcattgc gctcgtcacc atgcatcatt
     2461 gcaatcgcaa catgctcggc cttacggtgg atgtgagcac cgaggcagac gcattcgccg
     2521 gacgcgacgc gaactttgag ttcgtactgc gtaacgagtc caaggtcgat cggcgcgatg
     2581 tcgaaattcg ctgcctgacg ggcgccgctg tttgcagcgt gttggcacga tcgagcgaat
     2641 cggcgcgggt cgccatcccg gtggcgcagc gcggcgtgct gcgcattgca cagttcgagc
     2701 tgcgcacccg ctatcccttc ggctggtttc atgcgtggac ctacgtgcag gggtctttga
     2761 cggtgtatgt cgcgcccacc cccgcgggca cgcggacttt gccgcctgcg gccggcgcgg
     2821 gcagcgtggc agcgcgctcc gatgttcgcg gc
//
LOCUS       DASWZT010000443        18101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_66324, whole genome shotgun sequence.
ACCESSION   DASWZT010000443 DASWZT010000000
VERSION     DASWZT010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 18101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18101
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66324"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VGE92_12555"
     CDS             complement(<1..191)
                     /locus_tag="VGE92_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314707.1"
                     /translation="MTQQSPFEDIDPIETQEWLESIDSVLRQRGPQRAHFLLEKLVDF
                     TRRSGTYLPFKPNTAYLNT"
     gene            335..1864
                     /locus_tag="VGE92_12560"
     CDS             335..1864
                     /locus_tag="VGE92_12560"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HEY1314708.1"
                     /translation="MPTRLPFDQLLVPVRQIGARISAARLAGYSSALLFLPLAPSAAD
                     WKALPHGAVLRTLYARKVRKAGDCFHLRVGTEAQTLLIVACLAAEASTFERLQAAAKL
                     ARAALDGEPKSLLLWQQGCEAAAANASLSAAIAALEAAAFRFASFKSKPKPRSRLLRI
                     DLAAATKLPDLDVTLATAAGNNLARWLTALPPNTLDARGYRGYLQQIARRLKLGFKFY
                     GEAQLKRLGAGAFLAVSQGNGTRDAGIVHLAYRPGGAARARAPAVSLVGKGICFDTGG
                     TNLKPHKSMLDMHIDMEGSAVALGSLYALHALRSALSIDCWLAVTENRIGPLAYKPQD
                     VVRAYNGTTIQVIHTDAEGRMVLADTLGLAAARKPRAIIDYATLTGACVYALTERYSG
                     AFTNRPESRDLLESAGSSSGERVWCFPMDADFDTDIESQVADVLQCAADGKGDHILAA
                     RFLSRFVPKEIPWLHIDLAAGSRHGGLAHIATEITGFGVRYTVELLRRGWPQPAKRAR
                     K"
     gene            1861..2907
                     /gene="pyrC"
                     /locus_tag="VGE92_12565"
     CDS             1861..2907
                     /gene="pyrC"
                     /locus_tag="VGE92_12565"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY1314709.1"
                     /translation="MNALTLRRPDDWHVHLRDGAALAAVVKFTAQRFGRAIVMPNLKP
                     AVTTTASAGAYRDRILAALPKDTHFEPLLTLYLTDATAPEEIDRARASGFVHGVKLYP
                     AGATTHSDAGVTDIARVERVLARMQELGMPLLVHGETPGPDVDVFDRETHFIDAVLQP
                     LLERFPGLRVVFEHITTLRAVEFVSDARSGVAATITPQHLLHNRNAIFSGGIRPHYYC
                     LPILKRERDRQSLLRAATSGNPRFFLGTDSAPHERSAKQSACGCAGMFTAHAALELYA
                     EAFDSAGCLERLEGFASQFGADFYGLPRHDEAITLIRKSWSPPQSYAFDEGALVPYRA
                     GESVAWQIADVAYP"
     gene            2904..3584
                     /gene="rnt"
                     /locus_tag="VGE92_12570"
     CDS             2904..3584
                     /gene="rnt"
                     /locus_tag="VGE92_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HEY1314710.1"
                     /translation="MTQIEPITFATRFRGFLPVVIDVETGGFNSQTDALLEIAAVFLE
                     MRSDGTLARGETIRYHVTPFPGANLEPASLAVNGIDPHHPLRPAIPEEDALQRIFREV
                     RNAIRAADCTRAVLVGHNAFFDLNFLNAAVARAQVKRNPFHPFSSFDTATLGGVAFGQ
                     TVLMRATLAAGLDWDTASAHSAAYDAERTADLFCTVCNQFQGVFEASRRRLAAMGPLE
                     VEPSPEPP"
     gene            complement(3581..3907)
                     /gene="grxD"
                     /locus_tag="VGE92_12575"
     CDS             complement(3581..3907)
                     /gene="grxD"
                     /locus_tag="VGE92_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002907768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEY1314711.1"
                     /translation="MNAAVDRIKSQLAAAPVVLYMKGTPDFPQCGFSAQTVAALRACK
                     AQFAHVNIFEDPEVRETLKTYSNWPTFPQLYVNGELVGGCDIAIEMYKSGELKQLLAE
                     AGAVTA"
     gene            4091..4708
                     /locus_tag="VGE92_12580"
     CDS             4091..4708
                     /locus_tag="VGE92_12580"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEY1314712.1"
                     /translation="MNPLTTVKWPLILGFVLAVAIAALVGGGLSFNAATLASLTRWLH
                     IIFGIFWIGLLYYFNAVQTPAMAVAAADKGGPGGAGINKYVAPRALLWFRWSALLTWL
                     AGAYLLSQTHGRFINAFTFGLLENPVSRPNIVMGFGAWFGTIMLFNVWGLIWPNQKKI
                     LGIVPATDDEKAAARKIALYASRTNFLLSIPMLMCMIGPSHGLPF"
     gene            4672..5562
                     /gene="nadC"
                     /locus_tag="VGE92_12585"
     CDS             4672..5562
                     /gene="nadC"
                     /locus_tag="VGE92_12585"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEY1314713.1"
                     /translation="MHDRAFARAAVLRASIPVPADLPQQVERALQEDIGTGDLTAALI
                     PADRMGRATVITREPAIVCGIPYVNASFNAVDHRVRPTWQVAEGDAAVADQVLLSVEG
                     PARALLTAERTALNFLQLLCATATAAHAFAKLLEGTKCRLLDTRKTLPGLRSAQKYAV
                     RVGGGDNHRMGLFDGILIKENHIMAAGSIAAAVGAARRSAAAIPVEVEVENLSELKQA
                     IAARADIAMLDEFSLADMRQAVALNAAGSHPLKLEASGGINAASIREVAATGVDFISV
                     GSITKHVRAVDLSMRFEFRG"
     gene            complement(5585..8269)
                     /locus_tag="VGE92_12590"
     CDS             complement(5585..8269)
                     /locus_tag="VGE92_12590"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY1314714.1"
                     /translation="MAIAPHDPRHPDLSASDILSPQTWPPRGIRPAVGLDVICTKIAV
                     DFQSMTAATALGVIGANLELLREATRTDAAFHIHVDAESGLFNEIQVARGMLTTGNPE
                     QLQGQTFDVFPWLQSRLAPLRVSELRDTTKPRGDQGQDAAVWSHLGIGSVLMIGYFVA
                     GRAAGLLGIAGAQPRENWEVQLHLLMKLIGCSLSTGLDRIEIQRQLRDLEERNELALY
                     SANDGLWDFDTLNNRVYLSPRWKAMLGYDEADVGHAPDWRTLVHSDDMSRVQAAIRDH
                     VAGKTPLFESMHRMRHATGEWRWVISRAKARVDDKGRLLRLVGVELDVTERKLYEEAL
                     FREKESAQITLQSIGDGVITTDATGVIDYVNPVAEALTGWRLEDGQGRAIEEIFRAFH
                     EETCEPLENPLAVAIRRTRSIKSVRPMLLIRRDGNEIYVESTASPIRDGSGAVSGGVL
                     VFHDVSEARELNRRLSYHASHDVLTGLVNRREFENRMERALKSAKARETSYALCCLDL
                     DQFKIVNDTCGHSAGDALLGQVGALLKSKVRWRDTLARLGGDEFGILLESCSLDEAMR
                     TAETLREAVKNFKFTWEERTFRLGASIGVVPISADNADVASVLSAADSACQAAKEAGR
                     NRVHSFEENDIDLMRRRREMQWAARINNALEEGRFELFRQTILPLQIPDEGAHYELLL
                     RMRDEAGKIVSPDNFMTAAERYGITPSIDRWVIENAFRWLVSEADEREKLTMCSINLS
                     GQSLGDDKFLPYVIEQFHHSGLDATKICFEITETAAIASFSQANRFIQALKELGCKFA
                     LDDFGTGLSSFGYLKHFPVDYLKIDGSFVKEILHDPIDREMVRSINEIGHLTGKQTIA
                     EFAENQEIITMLQSMGVDYAQGYGVSQPQRVLKAANSA"
     gene            complement(8459..9208)
                     /locus_tag="VGE92_12595"
     CDS             complement(8459..9208)
                     /locus_tag="VGE92_12595"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY1314715.1"
                     /translation="MAAVEEKPDGGLRNTIYRQQTRSSRRLTRSRLLLYKIVVPLGLA
                     LLRLVWSWSRVTRVIGAEHIAASLQRAPSFIPVYWHQHQVFCVKHLLGQRRAGLNLGF
                     LISPSVDGEIGAMLVKKIGAAVIRGSSSHTGARALRDYYQALAHQGISPAITPDGPRG
                     PPWKFKPGAVLLAQLSGRPMIPLSYAASRAWKIKWDRFVIPWPLSRIAIAIGEPVFIP
                     KGLDAAALARAQLDMEQRLKALFEAARDALG"
     gene            9258..10556
                     /locus_tag="VGE92_12600"
     CDS             9258..10556
                     /locus_tag="VGE92_12600"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY1314716.1"
                     /translation="MQTLGPPPGPADGFDLGGNDESLARLGRYFAEFGDIYRVFAPAR
                     GVYNYVINHPDDIKRVLLSNHRNYTKGEGMDRVKILLGNGIMTSEGDFWRRQRRMMQP
                     SFHRRVIDRFSALIREVNDKFAARWAEKAARSEPINLSDDTSELTLEIVLRSIFGTDL
                     ARLEAQMGANPFAVVAKEPNRDLKFAFRFRSLTKLVQELIESRRRRPEEHFDFLSMLM
                     ATRDRDTGQAMSDKVLIDELLTLIVAGHETTAAALAWTWYLISQHPQTAHALQAEADR
                     TPADRTLDLDAAESLAFTHQVLQEALRLYPPGWLITRRTLEADELGGFPIAPRTDVFI
                     SPYMLHRHPAFWSDPEEFKPERFAGVDAAERHRFSYIPFAVGPRHCIGENIAMFEMLV
                     HVNAMSRRFHLTRAGDEPIELEAQINLRPRSNLMMMVQPR"
     gene            10553..12538
                     /locus_tag="VGE92_12605"
     CDS             10553..12538
                     /locus_tag="VGE92_12605"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HEY1314717.1"
                     /translation="MNDAGQTNYKTLLDILERNRNVDRVVTYVEGENSERRVAYGEVY
                     DRALGILYHLQAMGAQRGDRMIIFLNNNEQFLDGFWAAICGGITPVPLAVGISDEHRH
                     KLLRVARKLGKPLLYTDSKSLERLQTLAADVGESALFDELKSRAFLVESITDISRAGK
                     LYRPTPDDLAFIQFSSGSTSEPKGVMLTHGNLVANTLGATAVGKYNDQDITLSWMPLT
                     HDMGLIGFYLMQFANRVHIHLMPTELFVRRPLLWMQVASKKRVTITCSPNFGYRHFLK
                     VLGDRRLDGLDLSAIRQIYNGAEPISVPLCNEFMSTLAYTGLRHHAMYPVYGLAEASL
                     AVTFPEAGTDYRWIRVNRHKLGIGIAIELNPADARDALELICLGRAVPNTELRIADPT
                     RAALPAGVVGHILIRGASVTRGYFGDAEATAQAIDADGWLDTGDLGFLHEGSLYIAGR
                     GKEIIFINGQNYYPYDLENIAQRAPGLDLNKLVAAGVAKPGAQGEELVIFVLHRGSVA
                     QFLPTAAAVSRLINEHTGLEVAQVIPTKRIPKTTSGKVQRHLLEQAYIEGEFDADLNE
                     LQALRAAAGGAAHVSGTDLETRLQTICETALAGRRIDITDNLFEIGASSLKLIEIHEN
                     IDRDFPGLIDLTELFDHPTIADLAKHLQGKLTARPPS"
     gene            complement(12516..13385)
                     /locus_tag="VGE92_12610"
     CDS             complement(12516..13385)
                     /locus_tag="VGE92_12610"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEY1314718.1"
                     /translation="MKLDDSRRSDNVEDDRGSGGGIGRGAGVGIGTIVIVVIGYFMGV
                     NPSTLLSLLNGGGDPQAVSNSAPVNASTEANDPQIDFVRAILGETEDVWSAYFTNLGK
                     TYTKPKLVLFSGSVSSACGFASAAAGPFYCPGDQKVYIDLTFYRQLATEFGAPGDFAR
                     AYVIAHEVGHHVQNLLGITDKASQAEQTTGRVGANHVSVEVELQADCFAGVWAAQANN
                     ARKILEPGDLESGLKAASSVGDDTLQKREQGTVVPDSFTHGTSAQRVGWFRRGFEAGK
                     IDACDTFKTGGAL"
     gene            complement(13445..14296)
                     /locus_tag="VGE92_12615"
     CDS             complement(13445..14296)
                     /locus_tag="VGE92_12615"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY1314719.1"
                     /translation="MINFFSRFPLRALYVFSSFLYLLGFYVVRHRHGVIREQLEKVFP
                     ALSAAERERIHKQFLKNFCDVMVEVLKSVSMSENDMCNHVQILNLAVARRYLDAGQSV
                     MFVTSHLGNWEWLLQGVTLQLGYPVDAAYKPLHDQWAERLMLKVRSRFGARLVPAKEL
                     LADFLRRRGIVRALAMNADQAPVSTDKRYWTDFLGQETAFYIGAEQIARATRLPIIYV
                     RVRRTRRGYYQVELEPLWDGREATEPNTVTERYARICEVDVLKSPADWLWSYRRWRLK
                     KPLYGAD"
     gene            14391..14867
                     /locus_tag="VGE92_12620"
     CDS             14391..14867
                     /locus_tag="VGE92_12620"
                     /inference="COORDINATES: protein motif:HMM:NF025449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGDYG domain-containing protein"
                     /protein_id="HEY1314720.1"
                     /translation="MIAPVKPVPLFRSSGKRGYSSLIAGDPRRVVACKLEREIDVRFT
                     PVACMVQTSEGLVHAAPGDAIITGTAGEHWRVSHARFADKYRPVPPTIAGQAGRYASL
                     PNRIVAIPMTEAFDVLLADGVSRLSGVAGEWLVDYGDGSLGIVSQSIFASTYQIIT"
     gene            14872..15915
                     /locus_tag="VGE92_12625"
     CDS             14872..15915
                     /locus_tag="VGE92_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314721.1"
                     /translation="MALHQVIQRVLLIGAHVHSLPPPPQPQPQPPDALLPLIEAVAGP
                     HKRFDERAIHYGHRYRSGFWAIYMLSALAVLFAMMPLALGWDSARHALHPYAGVWAFG
                     EVLVICTVSTIYALGHRRDWQGQWLRARTTAELSWYLPILAPLLDFGAADADPNWYLR
                     VFDPGQHLRGADDVATLCAANESLARRQLAGAWADRDFVTRYARWTVDILDQQRRYHF
                     RTASKQNALQHRVHQVNTVLFALTAVGAMLHLAIHSMWLSMVTTLFPALGASLHGALA
                     QSEAYRLGTTSERLVAELQGAMERIREVLRDGDAATDVVAVKASIEAAIALILEEHQD
                     WHLLVRPHNLPLA"
     gene            16015..16353
                     /locus_tag="VGE92_12630"
     CDS             16015..16353
                     /locus_tag="VGE92_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314722.1"
                     /translation="MRKYIIVFMEQRMGVKRLTQIPGPEGEAWFESRDDDDALAYVSA
                     HFLQQVRTCVRAELTSMAIVPSNPGAPPRLVDDSLENPHFHEFYIRYNSRTKLLEVDF
                     ESTVTGLVNA"
     gene            16423..17028
                     /locus_tag="VGE92_12635"
     CDS             16423..17028
                     /locus_tag="VGE92_12635"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY1314723.1"
                     /translation="MRSGFSILRNAAALCACVLLLAAPCSRSAEDKPLTAILLVARTE
                     LPDSDFAGSVVLAMNHLGIAPTGIILNRPTRVAVAELFPDLKAIARLHDKVYFGGPVE
                     PMSIWFLFRAAKPPHRAIRAFDDVFLSGDRDLLLQLLGRDKPMGDLRIYVGHAGWAPG
                     QLEAEIDRGDWTLKRAYADAIFNGKSDRPWPAPQIAPPPTI"
     gene            17135..17998
                     /locus_tag="VGE92_12640"
     CDS             17135..17998
                     /locus_tag="VGE92_12640"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY1314724.1"
                     /translation="MAPNADTELLAHSASTWRIHFLFGQGGSSGEPKSQWKWIGKGEL
                     SADRDCLTIVGQRHRYFWFSAKQVIRVELQQICNVVAAGRLVKFEVKLASLEGERTEI
                     VRLRTSDAHTAQQIALALPTTRSAEFERAHHEKLSFDRSMEQLGTQPLVTWALVAANI
                     AWFLFVASQGGGVLAPQPGVIIHWGSNFGPLTLNGEWWRLFSSMFVHFGLVHLAFNMW
                     VLWSIGRMIERMFGSLHFALLYVFAGLCGSMASLWWHPNVQSAGASGAIFGLLGGLLA
                     FVLNPVSGDPA"
BASE COUNT         3312 a         5756 c         5513 g         3520 t
ORIGIN      
        1 atcgtattga ggtaggcggt attcggcttg aacggcaggt aggtgcccga gcggcgcgtg
       61 aagtccacca atttctcgag caggaaatgc gcacgttgcg gaccgcgctg tcgcagcacc
      121 gaatcgatcg attccagcca ctcctgcgtt tctatgggat cgatatcttc gaacggcgat
      181 tgttgggtca taaaatgcac acgcccacgt gtcctgttaa gatggtaggg acgttacagc
      241 gccgttgcgc taactgccac tgcattatca accggcgcta tcttcggggt ttggattttc
      301 acggtcaagc aagggatagg cccgcttttc agatatgccc actcgcttgc ccttcgacca
      361 gctgctagtt ccggtgcgcc agatcggcgc gcgcatcagt gccgcgcgac tggccggata
      421 ttcgagcgcg ctgctgttcc tgccgctggc gccgagcgcg gccgactgga aggcgctgcc
      481 gcatggcgca gtgctgcgga cgttgtacgc ccgcaaagta cgcaaagccg gcgactgttt
      541 tcatctgcgc gtcgggaccg aggcgcaaac cctgctcatc gtcgcgtgcc tggccgccga
      601 ggccagtact tttgagcgcc tgcaggccgc cgccaagctc gcgcgcgccg ctctggatgg
      661 cgaacccaaa tcgctgctgc tgtggcagca gggctgcgaa gcggctgccg cgaacgcctc
      721 gctgagcgcg gccattgcag cgctcgaagc ggcggcgttt cgtttcgctt cgttcaagtc
      781 caagccgaag ccaaggtcgc gcctgctgcg catcgacctc gctgcggcaa cgaaactacc
      841 cgatctcgac gtgacgctgg cgacggccgc gggcaacaat ctggcccgct ggctgacggc
      901 gctgccgccc aacacgctcg acgcgcgcgg ctatcgcggc tacctgcagc agatcgcgcg
      961 gcgtctgaag ctcggcttca aattctacgg cgaggcacaa ttgaagcgtc tgggtgccgg
     1021 cgcttttctc gcggtctcgc agggcaacgg cacccgcgat gccggcatcg tgcatctcgc
     1081 ctatcgtccg ggcggcgccg cgcgcgctag agcgcccgcg gtaagcctgg tcggcaaggg
     1141 catctgcttc gataccggcg gaacgaacct caagccgcac aagagcatgc tcgacatgca
     1201 catcgacatg gaaggcagcg ccgttgcgct ggggagcctg tacgcgttgc acgcgctgcg
     1261 ctcggcgttg tcgatcgact gctggctcgc cgtcacggaa aatcgcatcg ggccgctcgc
     1321 ctacaaaccg caagacgtgg tgcgcgcgta caacggcacg accatccagg tgatccacac
     1381 cgacgcggag ggccgcatgg tgctggcgga tacgctcgga ctcgccgccg cgcgcaagcc
     1441 gcgcgccatc atcgactacg ccacgctcac cggcgcatgc gtatatgccc tcaccgaacg
     1501 ctacagcggc gcatttacca accggcccga gtcgcgcgac ctgctcgagt ccgccggcag
     1561 cagcagcggc gagcgcgtgt ggtgctttcc catggacgcc gacttcgata cggacatcga
     1621 aagccaggtc gccgatgtcc tgcagtgcgc cgccgacggc aagggcgacc acatcctcgc
     1681 cgcgcgtttc ttgagccggt tcgtgccgaa ggaaatcccc tggctgcaca tcgatctggc
     1741 ggccggttcg cgccacggcg gacttgccca catcgccacg gaaatcaccg gcttcggcgt
     1801 gcgttacacg gtcgagttgc tgcggcgtgg ctggccgcag ccggcgaagc gcgcacgcaa
     1861 atgaatgcgc tcaccctgcg gcgtccggat gactggcacg tgcatctgcg cgacggcgcg
     1921 gctttggcgg cggtggtgaa atttaccgcg cagcgctttg ggcgtgccat cgtcatgccc
     1981 aatctcaagc ctgccgttac aaccaccgcg tcggcgggcg cgtatcgcga cagaatattg
     2041 gccgcgctgc ccaaagacac ccatttcgaa ccgctgctga cgctgtattt gacggatgca
     2101 accgctcccg aggagatcga ccgcgcgcgt gccagcggct tcgtccacgg ggtcaagctg
     2161 tatccggccg gcgcgaccac gcactccgat gccggcgtca ccgatatcgc ccgggtcgag
     2221 cgcgtgctcg cccgcatgca ggagctcggc atgccgttgc tggtgcacgg cgaaacgccg
     2281 ggcccggacg tcgatgtgtt cgaccgagaa acgcacttca tcgatgcggt gctgcagcca
     2341 ctgctcgaac gcttccccgg attgcgcgtg gtcttcgagc acatcaccac cttgcgtgcg
     2401 gtcgaattcg tgagcgatgc caggagcggc gttgccgcaa ccatcacgcc gcagcacctg
     2461 ctgcacaatc gaaatgcgat tttttccggc ggcatccgcc cgcactacta ctgcttgccg
     2521 atactgaagc gcgagcgcga ccgccagtca ttgttgcgcg ccgccaccag cggcaatccg
     2581 cgtttttttc tcggcaccga cagtgctccg cacgagcgca gcgccaagca aagcgcctgc
     2641 ggttgcgcgg gcatgttcac cgcgcatgcg gcactcgagt tgtatgccga ggcgttcgac
     2701 tcggccggct gcctcgagcg cctggaaggg tttgccagtc aattcggggc ggatttctac
     2761 ggcctgccgc gtcacgacga ggcgatcacc ttgatcagga agtcgtggtc gccgccgcaa
     2821 tcctatgcat tcgatgaggg cgcgttggta ccgtatcgcg caggcgagtc ggttgcctgg
     2881 cagatcgccg atgtggctta cccatgacgc aaattgagcc gattaccttc gcgacgcgct
     2941 ttcgcggttt tctgccggtg gtgatcgatg tggaaaccgg cggcttcaat tcgcaaaccg
     3001 atgcgctgct cgagatcgcc gcggtattcc tcgagatgcg cagcgacggc acgctggcgc
     3061 gcggcgaaac gattcgctat cacgtcacgc cgtttcccgg cgccaatctg gagccggcat
     3121 cgctcgccgt gaacggcatc gacccgcatc acccgctgcg ccccgcgatt cccgaagaag
     3181 acgcgctgca gcgcatattc cgcgaagttc gcaacgccat ccgcgccgcg gattgcaccc
     3241 gggcggtgct ggtcggacac aatgcgtttt tcgacttgaa ttttttgaac gccgcggtgg
     3301 cgcgcgcgca ggtcaagcgc aaccccttcc accccttctc gagtttcgac accgcgacct
     3361 tgggcggcgt cgccttcggg cagacggtgc tgatgcgggc aaccctggcg gcaggcctcg
     3421 actgggatac cgcttccgcc cactcggcgg cctatgacgc cgagcgcacc gcggacctgt
     3481 tctgcacggt gtgcaatcaa tttcaggggg tattcgaggc cagccgccgc cggctggccg
     3541 cgatgggccc gctcgaggtg gaaccctcgc cggagccgcc ttaggcggtg actgcgcccg
     3601 cttccgcgag cagctgcttg agctcgccgc tcttgtacat ctcgatcgcg atgtcgcaac
     3661 cgccgaccag ttcgccgttg acgtacagct gcgggaaggt cggccaattc gaataggttt
     3721 tcaaggtctc gcgaacttcc ggatcctcga aaatattcac gtgggcgaac tgcgccttgc
     3781 acgcccgcag cgcggcgacg gtctgcgccg aaaatccgca ctgcgggaaa tcgggtgtac
     3841 ccttcatata aagcaccacc ggcgcggccg ccagctggct cttgatgcga tcgactgctg
     3901 cgttcatgac ctgctctcca ccttcggttg actgatatta ccagcccggg cggcccgcgt
     3961 ctgtgccgaa agcgtcgttt attgacacct tggcctgtgg caagcgcctg acatcgttga
     4021 attattgggg tcagtcccct attcttccag gccgccgcag ccgcgcaccg gaaaaaaaca
     4081 aggaaatcgt atgaacccat taaccaccgt gaaatggccg ttgattctgg gctttgtgct
     4141 cgccgtcgcg atcgcggcgt tggtgggtgg gggcttgagc ttcaatgcag ccaccttggc
     4201 gagcctgacg cgctggctgc acatcatctt cggcattttt tggattggcc tgctgtacta
     4261 cttcaatgcc gtccagacgc ccgccatggc cgtcgcggcg gccgataagg gtgggccggg
     4321 cggcgccggg atcaacaaat atgtcgcgcc gcgcgctctt ctctggtttc gctggtcggc
     4381 gcttctcacc tggcttgcgg gcgcctacct cttgtcccag acccatggcc gtttcatcaa
     4441 tgcgtttacc tttggcctgc tcgagaatcc ggtgtcgcgc ccgaatatcg tcatgggttt
     4501 cggcgcttgg ttcgggacca tcatgctgtt caatgtgtgg ggtctcattt ggcccaatca
     4561 gaaaaagatt ctcggcatcg tgccggcaac cgacgatgag aaggccgcgg cacgcaagat
     4621 cgccctgtat gcgtcgcgca ccaactttct gctctccatt ccgatgctga tgtgcatgat
     4681 cgggccttcg cacgggctgc cgttctgagg gcatcgattc cggttcccgc cgacctgccg
     4741 cagcaggttg agcgcgcttt gcaggaggac atcggcaccg gggacctgac ggccgcgctg
     4801 ataccggcgg atcggatggg gcgtgccacg gtgataacgc gcgagccggc catcgtttgc
     4861 ggcattccgt atgtaaacgc cagcttcaac gcggtcgatc accgcgtgcg gccgacctgg
     4921 caagtggcag agggtgacgc cgcggtcgcc gaccaggttt tgctcagcgt cgagggaccg
     4981 gcccgcgcgc tgctgacggc cgagcgcacc gcgctcaatt ttttgcagct gctctgcgcc
     5041 accgccacgg cggcacacgc ctttgccaag cttttggagg gtaccaagtg tcggctgctc
     5101 gacactcgca agacgctgcc gggattgcgc tccgcccaga aatacgcggt gcgcgtcggc
     5161 ggcggcgaca atcaccgcat gggtttgttc gatgggatcc tcatcaagga aaatcacatc
     5221 atggccgcgg gatcgatcgc cgccgcggtc ggtgccgcgc ggcgttcggc cgcagcgatt
     5281 ccggtcgagg tcgaggtcga gaatttgtcc gaattaaagc aggccatcgc ggcacgcgca
     5341 gatatcgcca tgctggatga attctcgctg gcggacatgc gccaggcggt cgccttgaac
     5401 gccgccggct ctcacccgct gaaactggaa gcatccggcg gcatcaacgc cgccagcatt
     5461 cgcgaggtgg ccgccaccgg cgtcgacttc atctccgtcg gcagtatcac caagcacgtg
     5521 cgcgccgtcg atctttccat gcgcttcgag ttccgcggct gaatgagccg cgaggcgcag
     5581 tacctcaggc gctattcgcg gccttcagca cgcgctgcgg ttgcgacacg ccatagcctt
     5641 gcgcgtagtc gacgcccatg ctctgcagca tggtgatgat ctcctggttc tcggcgaatt
     5701 ccgcgatggt ctgcttgccg gtcaagtgac cgatttcgtt gatcgagcgc accatctcgc
     5761 ggtcgatcgg atcgtgcaaa atctctttga cgaagctgcc gtcgatcttc aagtaatcga
     5821 ccgggaagtg cttcagataa ccgaacgatg acaggccggt gccgaaatca tcgagcgcga
     5881 acttgcatcc caattccttg agcgcctgga tgaatcggtt cgcctgcgag aaactggcga
     5941 tggccgccgt ttcggtgatt tcgaaacaga tcttggtggc atcgagcccg ctgtgatgga
     6001 actgctcgat cacatagggc aggaatttgt cgtcgcccag gctttgcccc gatagattga
     6061 tggagcacat ggtcagtttc tcgcgctcgt cggcctcgga taccagccag cggaatgcgt
     6121 tttcgatcac ccagcggtcg atgctcggcg tgatgccgta gcgttcggct gcggtcatga
     6181 aattgtccgg cgagacgatt ttgcctgcct catcgcgcat gcgcagcagc aattcgtaat
     6241 gcgcgccctc atcggggatc tgcagaggca ggatcgtctg tcggaacaac tcgaatcgcc
     6301 cttcctcgag ggcattgttg atgcgcgccg cccactgcat ttcgcggcgc cggcgcatca
     6361 ggtcgatgtc gttctcctcg aaactgtgca cgcgattgcg gccggcctct ttggcggcct
     6421 ggcaggcgct gtccgccgcg ctcaacaccg acgccacatc ggcgttgtct gcggaaatcg
     6481 gcaccacgcc gatgcttgcg cccaagcgga aggtgcgctc ttcccacgtg aacttgaaat
     6541 tcttgaccgc ctcgcgcagg gtctccgcgg tgcgcatcgc ctcatccagc gagcagcttt
     6601 cgagcaggat gccgaactca tcgccgccca ggcgcgccag cgtgtcgcgc cagcggacct
     6661 tggatttcag gagcgcgccc acctggccga gcagggcatc gcccgcgcta tgaccacagg
     6721 tgtcattgac gatcttgaac tggtccagat cgaggcagca cagcgcgtaa gaggtttcgc
     6781 gcgccttggc gctcttgagc gcccgctcca tgcgattttc gaattcgcgg cggttcacga
     6841 gacccgtgag gacgtcgtgg ctggcgtgat agctcaagcg ccgattcagc tcgcgtgcct
     6901 cgctgacgtc gtggaacacg aggacgccgc ccgacaccgc gccgctgccg tcgcggatcg
     6961 gcgaggcagt actctcgacg tagatttcat tgccgtcgcg gcggatcagc aacatcggcc
     7021 gcaccgattt gatggatcgc gtacggcgaa tggccaccgc cagcggattc tcgagcggct
     7081 cgcaggtttc ctcgtgaaac gcccggaaaa tctcttcgat ggcgcggccc tggccgtctt
     7141 cgagacgcca acccgtgagc gcctccgcca ccggattcac atagtcgatc acgcccgtgg
     7201 catcggtggt aatgacgccg tcgccgatgc tctgcagggt gatttgcgcg ctctcttttt
     7261 cgcgaaacag cgcctcttcg tatagtttgc gctcggtgac gtcgagttcc acaccgacca
     7321 gtcgcagcaa gcgacccttg tcgtcgaccc gggccttggc gcggctgatc acccagcgcc
     7381 attcgccggt cgcatggcgc atgcggtgca tgctctcgaa cagcggggtt ttgccggcga
     7441 catgatcgcg aatcgcggcc tgtactcgcg acatgtcatc cgagtgcacc agggttctcc
     7501 agtccggtgc atgcccaacg tcggcttcat cgtaccccag catcgctttc cagcgcggcg
     7561 atagatagac ccggttgttc agggtgtcga agtcccacaa tccgtcgttg gcactgtaga
     7621 gggcgagttc gttgcgttcc tccagatcgc gcagttggcg ctggatctcg atgcggtcca
     7681 agccggtgga gaggctgcag ccgatgagct tcatcaacag atgcagctgt acctcccaat
     7741 tctcgcgcgg ctgcgcgccg gcgatcccga ggagtccggc ggcgcggccg gcgacgaaat
     7801 agccgatcat cagaaccgag ccgatgccta aatgactcca tacggcggca tcctggccct
     7861 gatcgccacg cggcttggtg gtatcgcgca actccgacac ccgcaagggc gccagacgcg
     7921 actgcagcca cgggaacaca tcgaaggtct gaccttgcaa ctgttcggga ttgccggtgg
     7981 tcagcatgcc gcgcgccact tggatctcgt tgaacagtcc ggactccgca tcgacatgaa
     8041 tatgaaaggc ggcatcggtg cgcgtcgcct cgcgcagcag ttccaggttc gcaccgatga
     8101 cgccgagggc cgtcgccgcc gtcatcgatt gaaaatcgac cgcgatcttg gtgcagatga
     8161 catcgagtcc cacggcgggt cgaatgccgc gcggcggcca agtctgcgga gacagtatgt
     8221 ccgaggcgga aaggtctggg tggcgtgggt cgtggggtgc tattgccatg cgccgttcaa
     8281 ggtcccagaa aatgggtggg ggttatgaat caatagtttg ccactggaat tagttgagat
     8341 atcgaatgcg tcacacagtt cctcggattg tcgcgtgcgc cacatacact tggattctcg
     8401 cataagttgt aaggaataca agctaagcga ttgtattgtt agggcttgaa gcctggtgct
     8461 aacccaacgc gtcacgcgcc gcttcgaata acgccttaag tcgctgctcc atatcgagct
     8521 gcgcgcgcgc gagagccgcc gcatccaatc ccttgggaat gaataccggt tccccgatgg
     8581 cgatcgcgat tctcgacaac ggccatggga tgacgaagcg atcccacttg atcttccagg
     8641 cacgcgaggc ggcatagctc aagggaatca tgggccgccc cgacaattgc gcgagcaaca
     8701 cggcgcccgg tttgaacttc catggcggac cccgcggtcc atcgggggtg atggcaggcg
     8761 agatcccctg gtgcgccaac gcctggtagt aatcgcgcag cgcccgggct cccgtgtggc
     8821 tcgaggatcc gcgaatgacg gcggcaccta ttttttttac gagcatggcg ccgatctccc
     8881 cgtcgacgga gggcgagatc aaaaatccca gattcaatcc ggcgcggcgc tgcccgagca
     8941 ggtgcttgac gcagaacacc tggtgttgat gccaatacac tggaatgaac gagggtgcgc
     9001 gttggagcga cgccgcaatg tgctcggcgc cgatcacgcg ggtgacgcgc gaccagctcc
     9061 acaccaggcg caacaacgcc aaacccaatg gcaccacgat tttgtacaaa agcagccgac
     9121 tgcgcgtcag ccgccggctc gagcgggttt gctgccggta aatcgtgttg cgcaggccgc
     9181 cgtcgggttt ctcttccaca gcagccattg tagcccggtg tcccgtgccc ccaggggcca
     9241 taagttacaa tgcgccaatg caaacccttg gaccgccccc cggccccgcc gacggcttcg
     9301 atctcggcgg caacgacgag tcgttggctc gcctcgggcg ctactttgca gaattcggcg
     9361 acatctatcg cgtgttcgcg cccgcacgag gcgtctacaa ctacgtcatc aaccaccccg
     9421 acgatatcaa gcgggtgctg ctctcgaacc atcgcaacta caccaagggc gagggtatgg
     9481 atcgagtcaa gatcctgctc ggcaacggca tcatgacgag cgagggcgat ttctggcgcc
     9541 gtcagcgccg catgatgcag ccctcgtttc atcgccgggt gatcgaccga ttcagcgccc
     9601 tgatccgcga agtcaatgac aaattcgccg cgcgttgggc cgaaaaggcg gcccgctccg
     9661 agccgatcaa cttgagcgac gacaccagcg aattgacgct cgagatcgtg ctgcgctcga
     9721 ttttcggcac cgacttggcg cgcctggagg cgcagatggg cgccaacccc ttcgccgtgg
     9781 tcgccaaaga accgaaccgc gacctgaagt tcgcctttcg gttccgctcc ctgaccaagc
     9841 tcgtccaaga gctgatcgag agccgccgcc gccgcccgga agaacatttc gattttttga
     9901 gcatgctgat ggcgacccgc gatcgcgaca cgggccaagc gatgagcgac aaagtgctga
     9961 tcgatgagct gttgactctg atcgtcgccg gtcacgagac caccgccgcc gcgctcgcct
    10021 ggacctggta tctcatcagt caacatccgc aaaccgcgca cgcgctgcag gccgaggcgg
    10081 atcgcacccc tgcggatcga accttggacc tcgacgccgc cgagtcgttg gcattcacgc
    10141 accaggtgct gcaggaggca ttgcggctgt atccgcccgg ctggttgatt acgcgacgca
    10201 cactcgaggc ggacgaactc ggcggcttcc caatcgcgcc gcggactgat gtattcatca
    10261 gtccgtacat gctgcatcgg catccggcgt tctggagcga cccggaggaa ttcaagccgg
    10321 agcgatttgc cggcgtagat gccgcagaac ggcaccgctt ctcctatatc ccatttgccg
    10381 tcggtccgcg gcattgcatc ggcgagaaca tcgcgatgtt cgagatgctg gtacacgtca
    10441 acgccatgtc gcggcgcttt catctcaccc gcgcgggcga cgaacccatt gaactggagg
    10501 cgcagatcaa tctgcgcccg cgatccaacc tcatgatgat ggtgcagcca cgatgaatga
    10561 tgccggacag acgaactaca agactttgct cgatatcctg gagcgcaatc gcaacgtcga
    10621 tcgcgtggtg acctacgtcg agggtgaaaa ttccgaacgg cgcgtggcgt atggcgaagt
    10681 ctatgaccgg gcgctcggca tcttgtacca cctgcaagcc atgggcgcgc agcgcggcga
    10741 caggatgatc attttcttga acaacaacga gcagtttctc gatggcttct gggcggcgat
    10801 ctgcggcggt atcacgccgg tgcccctggc ggtcggcatc agcgatgagc accggcacaa
    10861 actgctgcgc gtggcccgca agctcggcaa gccgctcctg tacaccgact cgaagagtct
    10921 cgagcggctg cagaccctcg ccgccgacgt gggcgagtca gcattgttcg acgaactcaa
    10981 atcccgcgcc ttcctggtcg aatccatcac cgacatctcg cgcgccggta agctgtatcg
    11041 gccgacgccc gacgatctgg cgttcatcca gttctcttct ggctccacca gcgagccgaa
    11101 aggcgtgatg ctcacccacg gcaatctcgt ggccaacact ctgggcgcga ccgcggtcgg
    11161 caaatacaac gatcaggaca tcaccttgag ctggatgccc ttgacgcatg acatgggcct
    11221 gatcggattt tatctgatgc aattcgccaa tcgcgtgcac atccatctca tgccgaccga
    11281 gctgttcgtg cggcggccgc tgctctggat gcaagtggcg tcgaaaaagc gcgtcaccat
    11341 cacctgttcg cccaattttg ggtaccggca cttcctaaaa gtgctgggcg accgccggct
    11401 cgatgggctg gacttgtcgg ccatccggca gatctacaac ggcgccgaac cgatttcggt
    11461 gccgctgtgc aacgagttca tgagcacgct cgcctatacc gggctgcggc accacgccat
    11521 gtatccggtg tatgggcttg cggaagcttc attggcggta acatttcccg aggccggcac
    11581 ggactaccgc tggatccgcg tgaatcggca caagctgggc atcggcatag cgatcgagct
    11641 caatccggcg gacgcgcgcg acgccctcga actcatttgc ctgggccgcg cggtgcccaa
    11701 taccgagcta cgcattgccg acccgacgcg cgccgccctg ccggcaggag tcgtcggcca
    11761 catcttgatt cgcggcgcga gcgtgacgcg cggctacttc ggcgatgccg aggccacggc
    11821 gcaggccatc gatgcggacg gttggctcga cacgggcgat ctgggtttct tgcacgaagg
    11881 ctcgctgtac attgccgggc gcggcaagga aatcattttc atcaacggtc agaactacta
    11941 tccctacgat ttggagaata ttgcgcagcg cgcgccgggc ctcgatctca acaaactcgt
    12001 ggcggcgggt gtcgccaaac cgggcgctca gggcgaggaa ttagtgatct tcgtgctcca
    12061 ccgcggcagc gtggcgcagt tcttgccgac ggcggcggcg gtcagccggc tgatcaatga
    12121 gcacacgggt ctcgaggttg cacaagtcat tcccaccaag cgtatcccca aaaccaccag
    12181 cggcaaagtg cagcggcact tattggaaca ggcgtatatc gagggtgaat tcgacgcgga
    12241 cctgaacgaa ctgcaagcgc tgcgcgcggc agcaggaggc gcggctcacg tgtcgggcac
    12301 cgatctcgag acgcgcctgc agacgatctg cgagacagcg ctcgcgggcc gcagaatcga
    12361 catcaccgac aacttgttcg aaatcggcgc aagctcgctc aagctgatcg agattcacga
    12421 aaacatcgac cgcgacttcc ccggcctgat cgatctgacc gaattattcg atcatccgac
    12481 catcgccgat ctcgcgaaac acttgcaggg caaacttaca gcgcgccccc cgtcttgaag
    12541 gtgtcgcaag cgtcgatctt gccggcctcg aagccgcggc ggaaccagcc gacgcgctgt
    12601 gcggaggtgc cgtgcgtaaa gctatcggga accaccgtcc cctgctcgcg cttctgcagc
    12661 gtgtcgtcgc ctacggaaga ggcggccttg agtcctgatt ccaaatcgcc gggctcgaga
    12721 atcttgcgcg cgttattggc ctgcgcggcc cacactcccg cgaaacaatc ggcctgcaac
    12781 tcgacctcga ccgacacgtg gttcgcgccg acgcgcccgg tggtctgctc cgcctgggag
    12841 gccttgtcgg tgatccccaa gaggttctgc acatgatgtc cgacttcgtg agcgatcacg
    12901 taggcgcgcg cgaagtcgcc cggcgcgccg aattcggtgg cgagttgccg atagaaggtc
    12961 aagtcgatgt acaccttctg gtcgcccggg caataaaacg gtccggcggc cgcgctggca
    13021 aaaccgcacg cggatgacac cgagccgctg aacaacacga gcttcggctt ggtgtaggtc
    13081 ttgcccaggt tcgtgaaata ggcgctccac acatcttcgg tctcgccgag gatggctcgc
    13141 acaaaatcga tttgcgggtc attcgcttcg gtcgatgcat tcacaggcgc cgagttgctg
    13201 accgcctgcg ggtcgccgcc gccattgagc aggctgagca atgtcgacgg attcacgccc
    13261 atgaaataac cgatgaccac gatcacgatg gttccgatgc caaccccggc accgcggcca
    13321 ataccgccgc cggatccgcg atcgtcctcg acattatcgc tgcgacggct gtcgtccaat
    13381 ttcatggcgc acctcggata attgttgttg agggaaaagc tcgaccctcg gtccgtatcc
    13441 taactcaatc ggcgccgtac aacggcttct tcagccgcca gcggcggtac gaccacagcc
    13501 agtcggccgg actcttgagc acatcgactt cgcagatgcg ggcgtagcgc tcggtcaccg
    13561 tattcggctc ggtcgcctcg cgtccgtccc acaatggctc caattccact tgataatagc
    13621 cgcgccgtgt ccggcgcacg cgcacataga tgatgggcag ccgggttgcg cgcgcaatct
    13681 gctcggcgcc gatgtaaaag gcggtctcct gtcccaagaa atccgtccag tagcgcttat
    13741 cggtggacac cggcgcctga tcggcattca tggccagcgc ccggactatc ccgcgccgtc
    13801 gcagaaagtc cgcgagcagc tccttggcgg gtaccagccg cgcgccaaat cgggagcgca
    13861 ctttgagcat cagccgctcc gcccattggt cgtgcagagg cttgtacgcg gcgtcgacgg
    13921 gataccccag ctgcagcgtc accccttgca gcagccactc ccagttgccg aggtgcgagg
    13981 tgacgaacat caccgattgg ccggcatcca agtagcgccg cgccacggcc aaattcaaga
    14041 tttggacgtg attgcacatg tcgttttccg acatcgatac cgatttcagc acctccacca
    14101 tcacatcgca gaaattcttg agaaattgct tgtggatgcg ttcgcgctct gccgcactca
    14161 acgcgggaaa gactttttcg agctgctcgc gaatgacgcc gtgccgatgg cgcaccacgt
    14221 agaaccccag gaggtacaag aagctggaaa atacatatag ggcgcgcagc ggaaagcgcg
    14281 agaagaaatt tatcattggc gggtcagttt acggcattcg cgctgctcct tgcagtgttc
    14341 attgcgctgc gtcacactga aaacacgcta agacacccag ggcacccgca gtgattgctc
    14401 cggtaaaacc ggtaccgttg tttcgctcca gtgggaaacg tggctactcg agcctgatcg
    14461 caggcgatcc gcgccgcgtg gttgcttgca agctcgagcg cgaaatcgac gtgcggttca
    14521 cgccggtggc gtgcatggtg caaacctccg agggtttggt gcatgccgcg ccgggcgatg
    14581 ccatcatcac gggcacggcg ggggaacatt ggcgtgtatc gcatgcgcga ttcgccgaca
    14641 agtaccgccc cgtgccgccg accatcgccg gccaagcggg acgctatgcc agcctaccga
    14701 atcgaattgt cgcgataccg atgacagagg cgttcgacgt cctgctggcc gatggcgtgt
    14761 cccgcttgag cggcgttgcc ggtgagtggc tggtcgacta cggcgacggt agcctcggga
    14821 tcgtttcgca atcgatcttc gcttccacct accagatcat cacttgatgt aatggcgctg
    14881 caccaggtca ttcaacgggt gttgctgatt ggcgcgcacg tgcactcgtt gccgccgccc
    14941 ccgcagccgc aaccgcagcc gccggacgca ctcctgccgc tgatcgaggc ggtcgcgggt
    15001 ccgcacaaac gattcgacga gcgcgccatc cattatggcc accgctatcg gagcggcttc
    15061 tgggccattt atatgttgag tgccctcgcc gtgctgtttg cgatgatgcc gctcgcgctt
    15121 ggctgggaca gcgcaagaca tgcgttacat ccttacgcgg gcgtgtgggc gttcggagag
    15181 gtgctggtca tctgcacggt gagcaccatt tatgcgttgg gtcatcggcg cgactggcag
    15241 ggccagtggt tgcgggcgcg caccacggcc gaactcagtt ggtacctacc gatactcgcg
    15301 ccgttactcg atttcggcgc ggcggatgcg gatcccaatt ggtacctgcg cgtattcgat
    15361 cccggacagc atctgcgcgg cgccgacgac gtcgccacgc tgtgcgcggc gaatgaatcg
    15421 ctcgcgcgcc ggcaactggc cggcgcctgg gccgaccggg atttcgtcac gcgctacgcg
    15481 cgctggaccg tggacatatt ggaccagcag cgccgctatc actttcgcac cgccagcaaa
    15541 caaaatgccc tgcaacatcg cgtgcatcag gtgaacaccg tcttgttcgc attgactgcc
    15601 gtcggcgcga tgctgcatct cgcaattcac tcgatgtggt tgtccatggt gacgacgctt
    15661 ttccccgcgc tcggcgcctc cttgcacggt gcactcgctc aatccgaagc ctatcgtttg
    15721 ggtaccacat ccgagcgctt ggtcgccgag ttgcaaggtg cgatggagcg cattcgcgag
    15781 gtgctcagag acggtgacgc cgcgaccgat gtggttgccg tcaaagcgtc gatcgaggcg
    15841 gcgattgccc tgattctcga ggagcatcag gactggcacc tattggtgcg accgcacaat
    15901 ctgccgctcg cataattctg tgatctagtt cactcgattg catgggtcga tgagacctcg
    15961 ttggggtcgc ccagacgccc ttgcgagtac aacaaaaatc ctcggaggca cgcaatgcgc
    16021 aaatacatca tcgtattcat ggaacaaagg atgggcgtca aacgcctgac tcagattccc
    16081 gggcccgaag gtgaagcctg gttcgagtcg cgcgatgacg acgacgccct cgcctacgtc
    16141 tccgcccatt tcttgcagca agtcagaacc tgcgtgcgtg ccgagctcac gagtatggcc
    16201 atagtgccga gcaacccggg cgcgccgccg cggctcgtgg atgactcgct cgagaacccg
    16261 cacttccacg agttctacat caggtataac tcgcgcacca agctgctcga agtcgatttc
    16321 gaatccacgg tcacgggcct cgtcaacgcc taggccgtgc tgcacgcatt gacgatccgc
    16381 taaactgatg aggccacacc ccggctagcg gagcccttgc cgatgagaag cggtttcagc
    16441 attctgcgca acgctgccgc actttgtgcc tgcgtgcttc tgttggcggc gccgtgctcg
    16501 agatctgccg aggacaagcc tcttaccgcg atcctcctcg tcgctcgaac cgaactaccc
    16561 gattccgatt tcgctggttc ggtggtgctg gcgatgaacc atctagggat cgcaccgacc
    16621 ggtatcatcc tcaaccggcc cacgcgggtc gctgtcgcgg agctctttcc agatctgaag
    16681 gcgatcgcac ggctgcacga caaggtgtac tttggcggcc cggtcgagcc catgtccatt
    16741 tggttcctgt ttcgcgcagc gaagccgccg caccgggcga tccgggcgtt tgacgatgtc
    16801 ttcttgagcg gggatcggga cttgctcctg caactcctcg gccgcgacaa gcccatgggg
    16861 gatctgcgga tctacgtcgg gcacgcgggc tgggcgccgg gccaactcga ggcggaaatc
    16921 gaccgcggcg attggaccct caagcgcgcg tatgcagacg caattttcaa cggcaagtcc
    16981 gaccgtccct ggcccgcgcc gcagattgcg cctccgccga ccatatgatg cgcagaggct
    17041 tccatgtcgc gtgcgttgtc aggcaaaccc aacactgtca cagatccatg cgtcgctttc
    17101 tcgatatcct gactccgaga tggagaaagg cgcagtggca cccaacgctg acacagaact
    17161 gctggcgcat tcggcgtcca cttggcggat ccatttcctg tttggtcaag gcggatcttc
    17221 gggcgagccc aagagccaat ggaaatggat cggcaagggc gaactctcgg ccgaccgaga
    17281 ctgcctgacg atcgtcggcc agagacatcg atatttctgg ttttctgcga agcaggtcat
    17341 ccgagtagaa cttcagcaga tttgcaacgt cgtggccgcg ggacgccttg taaagtttga
    17401 agtcaaactg gcaagcctgg aaggtgagcg aacagaaatt gtccgtctgc gcaccagcga
    17461 tgcgcacact gcgcaacaga ttgctttggc acttccgacg acgcgcagcg ccgagttcga
    17521 gcgcgcgcac cacgagaaac tttccttcga ccgctcaatg gagcagctgg gcacgcaacc
    17581 tctcgttacc tgggcgctcg tcgcggccaa tattgcctgg ttcctgttcg tcgcgagcca
    17641 gggcggcgga gtgctcgccc ctcaaccggg cgtcatcatc cattggggtt caaatttcgg
    17701 tcccctgacc ttgaacggtg aatggtggcg tttattcagc agcatgttcg tgcattttgg
    17761 tcttgtgcat ctggcgttca atatgtgggt gctttggagc ataggtcgga tgatcgagcg
    17821 gatgttcggg tcgctccact tcgcgctgct ctatgtgttt gctggactgt gtgggagcat
    17881 ggcaagcctt tggtggcatc ccaacgtgca gagcgccggc gcctccggtg ccatatttgg
    17941 gctcttgggg gggctgctcg catttgtctt gaaccctgtt tccggcgatc ctgcgtgatc
    18001 cttggtacta aatggaatcg agtcacgccc gtgactccta atggcactcc ggattgagac
    18061 ggatgatctt ggagcaggtg tccgcagtga cgaagtcgtg t
//
LOCUS       DASWZT010000444         1533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_80932, whole genome shotgun sequence.
ACCESSION   DASWZT010000444 DASWZT010000000
VERSION     DASWZT010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80932"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1246)
                     /locus_tag="VGE92_12645"
     CDS             complement(83..1246)
                     /locus_tag="VGE92_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEY1314725.1"
                     /translation="MANADRRRGQLADVRAFLGEVFGADLHAKRIDSLAGATLGVMQS
                     ASLAVAMIGQALALAKGLVTKHAIKQVDRMLSNNGIDVWDSFSRWVPHQLAGRQTALV
                     AMDWTDFDDDDQATLALHLVTGHGRAAPLLWLTVWKDELKNHRNDFEDACLRRLAELV
                     PADCRVTILADRGFGDQKLFAFLAELGFSYVVRFRGNIQVADADGTTKLAAEWVGKGG
                     RARKLTDARVTAKGQQVGAVVCVHAKDMKEPWCLATSERDATAATLINHYSKRWTIEP
                     QFRDTKDLRFGMGLSSTRIGEPMRRDRLLLVSAFATALLTLLGAVGESLGMDRLLKSN
                     TSKTRIHSLFRQGCMLYELIPTMPEHRFAPLMAKFAEAVAGSGLFPIAIAASK"
BASE COUNT          292 a          480 c          456 g          305 t
ORIGIN      
        1 acccaaggtt gccattggcc ggctactggg ccattctcga aagctgacgt tcgccgagct
       61 gctttggcgc tcaggcatcc cctcattttg aggccgcaat agcgattgga aaaagtccgg
      121 agccagcgac ggcttcggcg aatttcgcca tgagcggggc gaaccgatgt tcaggcatgg
      181 tcgggatcag ctcgtagagc atgcagcctt gccggaacag cgagtggatc cgggtcttgc
      241 tggtgttcga tttcaacagg cggtccatac cgaggctttc gccaacagcg cccagcagcg
      301 tcagcaaggc ggtggcgaag gcgctgacca acaataggcg atcacggcgc atcggttcgc
      361 cgatgcgggt ggacgaaaga cccatgccga agcgcaggtc ctttgtatct cggaactggg
      421 gttcaatcgt ccatcttttg gaatagtggt tgatcagcgt tgccgccgtc gcgtcccgct
      481 cgctggttgc cagacaccac ggctccttca tgtctttggc atgcacgcac acgacggcac
      541 cgacttgctg gcctttggcc gtcacgcgcg catccgtcag tttgcgggca cggccgcctt
      601 tgcctaccca ctcggcggcg agtttggtgg ttccgtccgc atcggcgacc tgaatgttgc
      661 cgcgaaagcg gaccacgtag ctgaagccca gttccgccag aaaggcgaac agcttctggt
      721 cgccgaagcc gcgatccgca aggatggtga cgcggcagtc ggcgggcacg agttcggcca
      781 gccgccgcag gcacgcatcc tcgaaatcgt tgcgatgatt cttcagttcg tccttccata
      841 cggtcagcca cagcagcggc gccgcgcggc catggcccgt gaccaggtgc aacgcgagcg
      901 tggcctggtc gtcgtcgtca aaatcggtcc agtccatggc caccagcgcc gtctgcctac
      961 cggcgagttg gtgcggcacc caacgggaga aactgtccca cacgtcgatg ccgttgttgc
     1021 tcagcatccg gtcaacttgc ttgatcgcgt gcttggtcac caaacccttt gccagtgcga
     1081 gcgcctgtcc gatcatcgcg accgcgagcg aggcggactg cataacgccg agcgtggcac
     1141 ccgccagcga gtcgatccgc ttggcatgca ggtcagcgcc aaacacctcg cccaagaacg
     1201 cgcggacatc tgccaattgt ccccgccgcc gatccgcatt tgccattctg ccgctccaac
     1261 ccataaacgg ctggaacatc tacggaatcg aggcgaatcg aggctagccc caagcgcaat
     1321 tataatgagg ggattcatga ggtcccaccg tgtttccagt gcccctcccg atccgagggg
     1381 aagcctgagg gtcagtatag accctgttct ttacttgtca atacattatt acttcctttt
     1441 cctgatgatg cgcctgtgag tatgtggaca acgcgatagc gtcgcgcggc gatgcggaag
     1501 cgttgtccac acactcacag gcgggcccgt tat
//
LOCUS       DASWZT010000445         2878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_61511, whole genome shotgun sequence.
ACCESSION   DASWZT010000445 DASWZT010000000
VERSION     DASWZT010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61511"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VGE92_12650"
     CDS             <1..781
                     /locus_tag="VGE92_12650"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY1314726.1"
                     /translation="LEVSRMPAERLQKIIAAAGIASRRKAEALISAGHVKVNGTVITQ
                     LGTKADPDTDHIRVDGKLLQGAQLHIYLLLNKPKGYVTTVSDPEKRPTVMQLLRGVKK
                     RVYPVGRLDYASEGLLLLTNDGTLANKLMKAASQVSKTYVVKVAGVPSNDALEKLRAG
                     VSIATEDGRRVKTAPAGLRLIKEATNPWYEVTLIEGRNREIHRMFEKVGHHVEKIKRV
                     RYGPLTLDVPPGKFRALTLQEVDRLRTACNKPVAAAKPSVA"
     gene            836..1957
                     /gene="hisC"
                     /locus_tag="VGE92_12655"
     CDS             836..1957
                     /gene="hisC"
                     /locus_tag="VGE92_12655"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY1314727.1"
                     /translation="MGVADLPVPPPRQPQQRSRLIKLDANESPFGPSPRALEALRSAL
                     SAANLYPDDDCTPLRLRLAAHHGLPAEQVMVTAGSTALLSLLCQTVLAPGLNAVTSER
                     SFIVYSLAVQAAGAHLVETPMRAGEDSFDLEAILAAIDSNTRIVFLANPNNPTGTVFE
                     AAAVERFLAQLPSHVLLVLDEAYYEFALHFAALGGIAYSNSLDYVRQGASVIVLRTFS
                     KAHGLAGLRIGYGLGPAELLAYCTRMRNTFSVSSVAQAAAMAALEDRLHIMKVVENNA
                     VQSLVLSEGLSALGFRVVPTAANFIFCDLEQDAASAAGQLKDEGVSVRPLGDWGAPHC
                     IRVTIGTAQQNQMFLSAMRRLAGHYNPGQRVAASLEPKE"
     gene            complement(1958..>2878)
                     /locus_tag="VGE92_12660"
     CDS             complement(1958..>2878)
                     /locus_tag="VGE92_12660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEY1314728.1"
                     /translation="VPAARASHAASVATVAAASSAPHGAKISPKARRLAKELGVDIAA
                     VRGSGAGGEILASDVQAATAPVADRASAGKTNIEIPTTLGRLMAERTAQSWTTVPHFF
                     VTREIEASALNEYRDKIVGEIKSSHKLRITHTDLLVALTARVLVKHPRLNSSFVAGEI
                     RLHHHVNMGVAIAVDDGVVAAVIPNAHAATLAEIATERRDVTERARVGKLRPADIADA
                     TFTISNLGMYHVDQFTAIIISPQAAILAVGGIAERVVAVAGKPAIRPMMTLTLSCDHR
                     VADGARAALFLSDLAEALREPEKVLRLATH"
BASE COUNT          588 a          926 c          826 g          538 t
ORIGIN      
        1 attggaagtt tcccgaatgc ctgctgaacg cctgcaaaaa attatcgccg ccgctggaat
       61 cgcctcccgc cgcaaagccg aagcactcat cagcgccggc cacgtcaagg tgaacgggac
      121 cgtaatcacc caactcggca ccaaggccga tcccgacacc gatcacattc gcgtcgacgg
      181 aaaacttctg caaggcgcgc aacttcatat ttacctgctg ctgaacaaac ccaaaggcta
      241 cgtcactacg gtgagtgatc cggaaaagcg gccaacagta atgcaattgc tgcggggcgt
      301 gaaaaaacgt gtatatccgg ttggccgtct cgactatgcc agcgaggggt tgctgctcct
      361 cacaaacgac ggcaccctcg ccaacaagct gatgaaagct gcgtcgcagg tctccaagac
      421 ttatgtagta aaagtcgcgg gagttccgag caacgacgcc ttggaaaaac tgcgcgccgg
      481 agtctccatc gcaaccgaag atggccgccg cgtaaaaacc gcgcccgcgg gcctccgcct
      541 gattaaagaa gccaccaatc cgtggtatga agtgactttg atcgaaggcc gcaaccgtga
      601 gattcaccgc atgttcgaga aggtcggcca tcatgtggaa aaaatcaaac gcgtccgcta
      661 cggccccctc accctcgacg tgcctcccgg aaaattccgc gcgctcacgc tgcaggaagt
      721 tgatcgcctg cgcaccgcct gcaacaaacc cgtagcggcg gcaaagcctt ccgtggcatg
      781 atgagcggca agcatgagcc gttttttcga catgatttcc aaacccgccc gcgacatggg
      841 cgtcgccgat ctaccagtcc cgccgccgcg ccagcctcag cagcgctcgc ggttgatcaa
      901 actggatgcc aacgagagtc ccttcgggcc gtcgccgcgc gccctcgaag ccctgcgcag
      961 cgccctgagc gcggccaatc tttatccgga tgacgattgc actccgctgc gcctcagact
     1021 cgccgcccat cacggcttgc cggccgagca ggtgatggtc accgccggct caaccgcgct
     1081 cctgagcctg ctctgccaga ccgttcttgc gcccggccta aacgcggtca ccagcgagcg
     1141 ctcctttatt gtgtattcgc tcgcggtgca ggctgcgggc gcgcatctgg tcgaaacgcc
     1201 catgcgcgct ggcgaggata gcttcgatct ggaggcaatc ctagccgcca tcgactcgaa
     1261 tacgcgcatc gtttttctgg caaatcccaa taatccaacc ggcactgtgt tcgaagcggc
     1321 cgcggtggag cgttttctgg cgcaacttcc cagccatgta ttgttggtgc tggatgaggc
     1381 gtactacgag ttcgccttgc atttcgccgc cctcggcgga attgcatact ctaactcgct
     1441 ggattatgtt cgccagggcg caagcgtgat tgtgctgcga accttttcca aagcgcacgg
     1501 cctcgccggc ctgcgcatcg ggtacggcct cggcccagcg gagttgctgg cctactgcac
     1561 ccgcatgcgc aacacttttt cggtgtcatc agtggcgcag gcagccgcga tggccgcgct
     1621 cgaagaccgc ctccacatta tgaaagtggt tgagaataat gcggtgcagt cgctggtcct
     1681 cagcgaggga ttgtcggcgc tgggttttcg cgtcgtgccc acggccgcga actttatctt
     1741 ctgcgatctt gaacaagacg cagcctccgc cgccggtcaa ttgaaggacg agggcgtgtc
     1801 ggtgcgaccg ctgggagatt ggggcgcgcc ccattgcatc cgcgtgacga tcggcacagc
     1861 gcagcagaat cagatgttcc tcagtgccat gcggcgactc gcgggacatt acaacccagg
     1921 tcagcgggtc gcagcctcgc tcgaaccgaa agaatgatta atgagttgca aggcgcagaa
     1981 ctttctccgg ttcgcgcagc gcttccgcca gatcgctaag aaaaagcgcg gcgcgggcac
     2041 catcggccac gcgatggtcg cacgataaag tcagcgtcat catgggtcga atcgcgggtt
     2101 tacccgccac cgccacgact cgctcggcga tgccgccgac cgcgagaatc gccgcttgcg
     2161 gcgaaataat gatggcagtg aactgatcca cgtgatacat gccaagattg ctgatggtga
     2221 acgtggcgtc ggctatatcg gcgggacgca acttgcctac ccgcgcgcgc tcggtaacat
     2281 cgcgccgctc tgtcgcgatt tcggccaggg tcgccgcgtg agcattcgga atcacggccg
     2341 cgactacgcc gtcgtctacc gcgatcgcca cgcccatgtt cacatgatgg tgcagccgaa
     2401 tctcccccgc aacaaagctc gaattcaagc gcggatgctt gaccaacacg cgcgccgtca
     2461 gcgccaccag caaatccgtg tgcgtgatgc gaagtttgtg cgaactcttg atctcgccca
     2521 cgattttgtc gcgatattcg ttgagcgcgc tggcttcgat ttcccgcgtc acaaaaaaat
     2581 gaggcacggt ggtccagctt tgagcggtgc gttcggccat caaacgcccg agagtcgtag
     2641 gaatttcgat attcgttttt ccggcagagg cacgatcggc aaccggcgca gtcgcagctt
     2701 gcacgtctga cgccagaatc tccccgcccg cgccggagcc gcgcactgca gcgatatcaa
     2761 cgcccaactc cttcgccagt cgccgcgcct tcggagaaat cttcgcgccg tgaggtgcgg
     2821 aggacgcggc agcaacggtc gccaccgacg cagcatggga cgcgcgcgcc gccggaac
//
LOCUS       DASWZT010000446         6588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_51834, whole genome shotgun sequence.
ACCESSION   DASWZT010000446 DASWZT010000000
VERSION     DASWZT010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6588
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51834"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VGE92_12665"
     CDS             complement(<1..428)
                     /locus_tag="VGE92_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY1314729.1"
                     /translation="MSKGKFERTKPHVNVGTIGHVDHGKTTLTAALTKVMAETNGGAY
                     VAYDQIDKAPEEKARGITISTAHVEYQSESRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPYIVVALNKVDMVD"
     gene            complement(469..544)
                     /locus_tag="VGE92_12670"
     tRNA            complement(469..544)
                     /locus_tag="VGE92_12670"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(509..511),aa:Thr,seq:ggt)
     gene            complement(555..628)
                     /locus_tag="VGE92_12675"
     tRNA            complement(555..628)
                     /locus_tag="VGE92_12675"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(594..596),aa:Gly,seq:tcc)
     gene            complement(667..752)
                     /locus_tag="VGE92_12680"
     tRNA            complement(667..752)
                     /locus_tag="VGE92_12680"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(716..718),aa:Tyr,seq:gta)
     gene            992..1531
                     /locus_tag="VGE92_12685"
     CDS             992..1531
                     /locus_tag="VGE92_12685"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY1314730.1"
                     /translation="MEGGDMGNRAFGMGRKLASVDLQLLILGLLAEKPRHGYEIIKAL
                     DERSKGFYVPSPGMVYPALTYLEEIGHATVEVDGTRKLYHITDAGKEHLKTNQSAAEA
                     MFAQFGRVGERMDRVRRAMRADQTGEEADLERKGSKELQRARKDLKLALADKWDATAA
                     EQQRIVDILKRATGEILGN"
     gene            complement(1581..4019)
                     /locus_tag="VGE92_12690"
     CDS             complement(1581..4019)
                     /locus_tag="VGE92_12690"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY1314731.1"
                     /translation="MRPLDVLIAVVCALLQAAAAAAAPQDLALEKIMADPAWVGPRAK
                     DGYWSGDGRSVYYSLQRAASPIVDLHRIDLKDRKDQTVEPQALAAADGPPVFDAAGKR
                     AAFVRSGDIFVRDLASGRLTQITRSAQLKAAPQFSADGGLLSFRVGNDWFVHDFGSGV
                     TAQAAVVKTEKDPDAAPKPDDLRDMQLRTFSTLKKLHDEADALKKHAAELRSGDATQA
                     AAAFYIGDDLTIRDTELSPDAHWLAIVAEPKSAAKGFEGKLTRYVTESGYEEFETERT
                     RVGRNIPASHSLLLLNLVDHTVHTLAFDTLPGIADDPLQAIRAENGTQKAAGAADSGK
                     DKPQLRAVSLFSDDPENSGGGVVWSSDGKALAVELLSVDNKDRWIASVDFSRYALIPQ
                     HRLSDPAWVNWDFNEFGWLKDNRTLWFESEQSGYAHLYTKAPNAAARALTQGQFEVSA
                     PTLSADGRWFYVLANAVAPYSYDVYRVASGGGELQRITHLQGLEKFTLDASGKRLLLT
                     HSSPYIPAQLAIQNADGSGVASELTDTRPAAYKSLSWIEPQIIKIPSSHIDGTIYAKL
                     YRDAPAAGAMAAHAGVIFIHGAGYTQNVSLKFPYYFREQMFNNWLVQHGYIVLDLDYR
                     ASAGYGRDWRTAIYRQMGHPELEDLLDAKRWLVQQAAVDAKRIGLYGGSYGGFMTLMA
                     LFRAPGEFAAGAALRPVTDWMQYEQGYTSSILNDPQSDPIAYKRSSPLEFADGLRDPL
                     LICHGVIDDNVLFEDSMRLYERLIELHKDNFTISPYPLDRHGFTNADSWLDEYKRIYR
                     LFEANLKTSAPRQQ"
     gene            complement(4006..4500)
                     /locus_tag="VGE92_12695"
     CDS             complement(4006..4500)
                     /locus_tag="VGE92_12695"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1314732.1"
                     /translation="MAPMYRLVEAHSKLEFKAARALIEEYAGEIGDSMGVDLAFQGLD
                     IELERLPEMYGPPTGALLLASRDHEWVGCCALRRFAEGVCEMKRLYIRPQVRGANLGR
                     QMSEHLLLQARALGYRRMVLDTLQDMIAAQALYRSLGFRDTEPYYFNPMAGVTYMELD
                     LEAS"
     gene            complement(4512..5912)
                     /locus_tag="VGE92_12700"
     CDS             complement(4512..5912)
                     /locus_tag="VGE92_12700"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEY1314733.1"
                     /translation="MSARNLEEILNAAGSSVELLRNSQLGAYVYPVVAPEFSNWRDEQ
                     RAWRETAVLFDQSHHMVDLFIEGADAAKLLSHLAINSFVGFEVNKAKQFVPCNYDGQV
                     IGDGILFHLAPDKFVFVGRAPSANWIMFHAATGGYDVRVVKDDRSPSRPMGKPVVRKF
                     YRFQIQGPNAKAIIEKVNGGPFPDIKFFSIGSINIAGRKVSALRHGMAGAPGLEVWGP
                     YEESEEIRAAILEAGRDLGLRQVGARAYATNTLESGWIPSPLPAVYSGAKMRAYREWL
                     PATSYEATGSLGGSFVSNKIEDYYLNPYELGYGPFVKFDHDFIGREALEKLDKGAQRR
                     KVTLAWNGDDVAKVFASLFNPGAPHYKYIDLPLSNYASSNYDRVSVGGKTVGFSMFTG
                     CSYNERSMLSLATLDPDVAIGNEVSVVWGEENGGTRKTTVEPHQQIEIRAIVSPVPYS
                     KVAREEYAKGWRTARA"
     gene            complement(5925..6332)
                     /locus_tag="VGE92_12705"
     CDS             complement(5925..6332)
                     /locus_tag="VGE92_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314734.1"
                     /translation="MNISDLIAIDVHTHAEVSCRQPADEVWQAYETAATKYFKADKRL
                     TIAETVAHYRQQKIGLVMFTVDSGNSDIMIAFASIDPHKGKMGVREARRVDPQGQCGQ
                     LARSRMRRAHDASLAWAARGQACARIIHPELAV"
     gene            complement(6338..>6588)
                     /locus_tag="VGE92_12710"
     CDS             complement(6338..>6588)
                     /locus_tag="VGE92_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314735.1"
                     /translation="NAGVVGLYSLMAQVFPTHVRATGTGFAIGFGRAGAWLSPIIAGY
                     LFHTGHGLGFVAIAMGTGSLLAVIALWLLRFEPHAAAI"
BASE COUNT         1229 a         2125 c         1958 g         1276 t
ORIGIN      
        1 tcgtccacca tgtcgacttt gttcaaagcc accacaatgt acggcacacc cacctggcgc
       61 gccaacaaaa tgtgctcccg cgtctggggc atcgggccgt ccgccgccga taccaccaat
      121 atcgcaccgt ccatctgcgc cgctcccgtg atcatgttct tcacgtagtc cgcgtgcccg
      181 ggacagtcca cgtgcgcgta gtgccggctc tcgctctggt actccacgtg cgccgtggag
      241 atcgtgatgc cgcgcgcctt ctcctcgggt gccttgtcaa tctggtcgta cgccacgtac
      301 gccccgccat tggtctcggc catcaccttg gtcagcgccg ccgtcagcgt cgtcttgcca
      361 tgatccacat gcccaatcgt ccccacgttc acgtgcggct tcgttcgctc aaacttcccc
      421 ttcgacacgg caagtacccc tcagaatttg acaagcaata ataaaaactg gagcccacga
      481 acgggatcga accgttgacc tcgtccttac caaggacgtg ctctaccaac tgagctacgt
      541 gggccgcatc catttggagc gggtgatggg aatcgaaccc acgtgatcag cttggaaggc
      601 tgacgttcta ccattgaact acacccgctt tccgctagag ccaatggcgc gaaccccaca
      661 tcctgctggt ggagggggta ggattcgaac ctacgaaggc ataagccagc agatttacag
      721 tctgcccccg ttggccgctt gggtacccct cctcaaaatg agccgcgtat tttgcggccc
      781 gcgtcacgcg gtgtcaatgt caagtcggac ctaaatcctt ggaccttccc ggctctgcct
      841 gcccggtcgt tcgtgcttga cactatcgta cgatatatat atcttacgat acgtcttaca
      901 acatatctgg agatatatgc atcatcgttt acatcactat tttgggcggc accatggcgg
      961 tcgcgggttc ggccccttcg gacgtggctt catggaaggc ggcgatatgg gcaaccgtgc
     1021 cttcggcatg ggccgtaaat tggcctcggt cgatctgcag ctgctcatat tggggctcct
     1081 cgcggaaaag ccccgccatg gctacgaaat catcaaggct ttggacgaac gctccaaggg
     1141 tttctatgta ccgagccccg gcatggtgta cccggcgctc acttatcttg aggaaatcgg
     1201 tcacgccacc gtcgaagtcg acggcacgcg caagctctac cacatcaccg atgccgggaa
     1261 ggagcacctg aagaccaacc agagcgccgc cgaggcgatg ttcgcgcagt tcggccgcgt
     1321 cggcgaacgg atggaccgcg tacgccgcgc catgcgtgcc gaccaaaccg gcgaggaggc
     1381 agacctcgag cgcaagggat ccaaggaact gcagcgcgca cgcaaagact tgaagctcgc
     1441 gctggccgac aaatgggatg ccaccgctgc agagcaacag cgcatcgtcg acatcctcaa
     1501 gcgcgcaacc ggggagattc taggcaattg acgcgtggct gccgcgcgcc ctggtgttcg
     1561 cggcggtcct gtgcattgct tcattgctgc cggggcgcgg aagttttgag attggcctcg
     1621 aacaaccggt aaattctctt gtactcatcc agccaggaat cggcattggt gaatccatgt
     1681 cgatccagcg ggtacggcga gatcgtaaaa ttgtccttgt gcaattcgat cagccgctca
     1741 tacaaacgca tcgagtcttc gaacagcacg ttgtcgtcga tgacgccatg acagatgagc
     1801 aagggatcgc gcaatccgtc ggcaaattcg aggggcgatg aacgcttgta ggcgatggga
     1861 tcgctttgcg gatcgttgag aatcgacgat gtgtaaccct gctcgtactg catccagtcg
     1921 gtcaccggac gcagcgccgc gccggcggcg aattctcccg gcgcccgaaa cagcgccatc
     1981 aatgtcatga agccgccgta ggatccgccg tacagaccga ttcgcttcgc atccaccgcc
     2041 gcctgctgca ccagccatcg cttggcgtcg agcaggtctt cgagctcggg atggcccatc
     2101 tgccgataga tggccgtgcg ccaatcgcgt ccgtagccgg cggaggcgcg gtagtcgagg
     2161 tcgagcacga tgtagccgtg ctgcaccagc cagttgttga acatctgctc gcggaaatag
     2221 taggggaatt tcaggctgac attttgcgta tagcccgcgc cgtggatgaa aatcacgccc
     2281 gcatgcgctg ccatcgcgcc cgccgccggg gcatcgcgat aaagctttgc atagatagtg
     2341 ccgtcgatgt ggctcgaggg tatcttgatg atctgcggct cgatccacga cagcgacttg
     2401 taagcggcgg gacgcgtatc ggtcagctcg ctcgccactc ccgaaccgtc cgcattctga
     2461 atcgcgagct gcgcagggat gtagggggat gaatgggtga ggagtaaccg cttgccggat
     2521 gcgtcgagcg tgaacttctc gagcccctgc aggtgcgtga tccgttgcag ctcgccgccg
     2581 ccgctcgcta cgcggtagac atcataggaa tagggcgcca cggcgttggc gagcacatag
     2641 aaccagcggc cgtccgcgga cagcgtcggc gcggacactt cgaattggcc ctgcgtcagc
     2701 gctcgcgccg cggcattcgg cgccttggtg tacaagtgag catagccgga ctgctccgac
     2761 tcgaaccaaa gcgtgcgatt gtccttgagc cacccgaact cgttgaagtc ccaattgacc
     2821 cacgccggat cgctcaatcg atgttgcggg atgagcgcat agcggctgaa atccacgctc
     2881 gcaatccagc gatccttgtt gtcgaccgac agcagttcca cggcgagcgc cttgccgtcg
     2941 ctgctccaga ccacgccgcc gccgctgttc tcgggatcgt ccgagaacag cgacacggcg
     3001 cgcagctgcg gcttgtcctt gccgctgtcg gctgcgcccg ccgccttctg cgtgccgttc
     3061 tctgcgcgaa tcgcctgcaa cgggtcgtcg gcaatgccgg gcagcgtgtc gaacgccagg
     3121 gtatgcaccg tgtgatcgac gagattcaac agcagcaggg agtgcgaggc cgggatattg
     3181 cggccaaccc gggtgcgctc cgtttcgaat tcctcgtatc ccgattcggt gacatagcgg
     3241 gtgagcttgc cttcgaatcc cttggccgcg gatttcggtt ccgccacgat ggcgagccaa
     3301 tgcgcatcgg gcgagagctc ggtgtcgcga atcgtgaggt catcgccgat atagaaagcc
     3361 gccgcggcct gcgtcgcatc gccgctgcgc agctccgccg catgtttttt gagcgcgtcg
     3421 gcttcgtcgt gcagcttctt caaggtcgag aaagtgcgca gctgcatgtc gcgcaagtcg
     3481 tcgggcttgg gcgcggcatc cggatccttt tcggtcttga cgacggccgc ctgcgcagtg
     3541 acgccgctgc cgaaatcgtg cacgaaccaa tcgttgccga cgcgaaagct cagcagccct
     3601 ccgtccgcgg agaactgcgg cgccgctttc aattgcgcac tgcgtgtaat ttgcgtcagc
     3661 cggccgcttg cgaggtcgcg gacaaaaata tcgccgctgc gcacgaaggc cgcgcgtttg
     3721 ccggcggcgt cgaacaccgg cggcccatcg gcggcggcga gcgcctgggg ttcgaccgtt
     3781 tgatctttgc gatccttgag atcgatgcgg tgtaaatcga cgatcggcga agccgcgcgc
     3841 tgcaacgagt aataaacgct gcggccgtcg ccggaccagt agccgtcctt cgcccgcggc
     3901 ccgacccaag cggggtccgc catgattttt tcgagcgcca ggtcctgggg cgccgccgcc
     3961 gcggcggcgg cctgcagcaa cgcgcagacc accgcgatca aaacgtcaag aggcctcaag
     4021 atccaactcc atataggtga ccccggccat cggattgaag tagtagggtt cggtgtcccg
     4081 aaatcccaac gaacggtaca gcgcctgggc ggcaatcata tcttgaagcg tgtcgagcac
     4141 catgcgccgg taaccgagcg cgcgcgcctg tagcagcagg tgttcgctca tttgacggcc
     4201 gagattggcg ccgcggacct gcggcctgat atagagccgc ttcatctcgc atacgccctc
     4261 tgcaaaacgg cgcagcgcgc agcaccccac ccactcgtga tcgcggctcg cgagcaacag
     4321 agcgcccgtc ggcggcccgt acatctcagg caagcgctcg agttcgatgt ccaacccctg
     4381 gaaagccaga tccacgccca tcgagtcgcc tatctctccg gcatattcct cgatgagcgc
     4441 tcgcgccgcc ttgaactcaa gcttgctgtg cgcctccacc aaacggtaca tcggggccat
     4501 ggttctctaa gtcacgcgcg agcggtgcgc cagcctttcg cgtactcctc gcgcgccacc
     4561 ttcgagtagg gcaccgggct tacgattgcg cgaatctcga tctgctgatg cggttcaacc
     4621 gtcgtcttgc gggtgccgcc gttctcctcg ccccacacga cgctcacttc gttgccgatc
     4681 gccacgtccg gatcgagcgt ggcgagcgac agcatcgagc gctcgttgta gctgcaaccc
     4741 gtgaacatcg agaagccgac ggtcttgccg ccgacactca cgcggtcata gttcgacgag
     4801 gcgtaattgg acaacggcag atcgatgtac ttgtagtgtg gcgcgcccgg attgaacagc
     4861 gaggcgaaca ctttggcgac atcgtcgccg ttccatgcca gcgtgacttt cctgcgctgc
     4921 gcgcccttgt cgagcttctc gagcgcctcg cggccgatga aatcgtgatc gaatttgacg
     4981 aacgggccgt agccgagctc gtaaggattc aggtagtaat cctcgatctt gttcgagacg
     5041 aagctgccgc cgagacttcc cgtcgcttca tagctcgtgg caggcagcca ttcgcggtag
     5101 gcgcgcattt tcgcgccgct atacacggcg ggcagtggcg aaggtatcca accggactcg
     5161 agggtattgg tcgcataggc gcgcgcgccc acctggcgca gacccaggtc gcgccccgcc
     5221 tcgagtatcg ccgcccggat ctcctcgctc tcctcgtagg gcccccacac ctcgagtccc
     5281 ggcgctcccg ccatcccatg gcgcagcgcg ctcaccttgc ggccggcgat gttgatgctg
     5341 ccgatgctga agaacttgat gtccgggaac ggcccgccgt tcaccttctc gatgatggcc
     5401 ttggcattcg gaccttggat ctggaagcga tagaacttgc gcaccaccgg tttgcccatc
     5461 gggcgcgacg gtgaacggtc gtccttcacg acccgtacat cgtagccgcc ggttgcggca
     5521 tggaacatga tccagttcgc cgacggcgcg cgtccgacga agacgaattt gtcgggcgcc
     5581 aagtgaaaca agatgccgtc gccgatgacc tgcccgtcat agttgcacgg cacgaattgc
     5641 ttggccttgt tgacttcgaa tccaacgaag ctgttgatcg cgaggtgcga cagcagcttg
     5701 gcggcgtccg cgccttcgat gaaaagatcg accatgtggt gcgattgatc gaacaacacc
     5761 gcggtttcgc gccacgctcg ctgctcgtcg cgccagttgg agaactccgg cgccaccacc
     5821 gggtagacgt aagcgcccag ctgcgaattg cgcagcagct cgacgctgct gccggccgca
     5881 ttgagaattt cttcgagatt tcgtgcgctc atgtccgccc ccctttagac agccaactcc
     5941 ggatggatta ttcgcgcaca ggcctggccg cgcgcagccc acgcgaggct agcatcatgc
     6001 gcccgcctca tgcgagaccg agcaagctgg ccgcattgtc cttgaggatc aacccggcgc
     6061 gcctcgcgca cacccatctt tcccttgtgc ggatcgatgc tcgcaaacgc gatcatgatg
     6121 tcgctgttgc ccgaatcgac cgtgaacatg accaagccga ttttctgctg ccgataatgt
     6181 gccacggtct cggcgatggt caggcgcttg tcggccttga agtacttcgt ggccgccgtc
     6241 tcgtaggcct gccagacttc gtcggccggc tgccggcacg acacttccgc atgggtgtgt
     6301 acgtcgatgg cgatgagatc gcttatgttc atggccgtca gatcgccgcc gcatgcggct
     6361 cgaagcgcaa cagccacagt gcaatgacgg ccaacagcga ccccgtgccc atcgcgatcg
     6421 cgacgaaccc gagcccgtga cccgtatgga acaaatagcc ggcgataatg ggcgacagcc
     6481 acgcgcccgc gcgtccgaat ccgatcgcga acccggtacc ggtcgcgcgc acatgcgtcg
     6541 ggaacacctg cgccatgagg ctgtatagcc cgaccacccc ggcgttgg
//
LOCUS       DASWZT010000447         1905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_32780, whole genome shotgun sequence.
ACCESSION   DASWZT010000447 DASWZT010000000
VERSION     DASWZT010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32780"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VGE92_12715"
     CDS             complement(<1..455)
                     /locus_tag="VGE92_12715"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY1314736.1"
                     /translation="MTIEVRVPQLPESVADATIVSWHKKPGDWVARDENLVDLETDKV
                     VLEVPAPAAGVLKEITLKDGTSVTSGQVLAILEEGATALPGASAPAIMASDSAATTAK
                     ESTESVAGKLSPAAKRVLEENQVDPKAVTGSGRDGRVSKSDVVNYLSAK"
     gene            complement(455..>1905)
                     /locus_tag="VGE92_12720"
     CDS             complement(455..>1905)
                     /locus_tag="VGE92_12720"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEY1314737.1"
                     /translation="YQVIRKKPTARQLYAEKLAAEGVLSAAEATAMIEQYRNGLDEGK
                     PQARAALGLIGNKFTVDWSEYLGADWGEPVKTAIDMGRLRALGKAITTYPSDWVLHPR
                     VLAIMQARERMVNGDLALDWGAAENLAYASLVQEGYPVRLTGQDSGRGTFFHRHAVLH
                     DQVTGRLYTPLQHLATNQPTFTVTDSVLSEEAVLGFEYGFSTTEPHCLTIWEAQFGDF
                     CNGAQVIIDQFITSGEAKWGRLSGLTLFLPHGYEGQGAEHSSARLERFLQLCAEFNMQ
                     VCVPSTPAQMFHMLRRQMVRHLRKPLIVMTPKSLLRHPLSVSRLEELSSGGFYSVIDE
                     VDDIKPAAVTRLVLCSGKVYFDLLKARREVKVDTVAIVRLEQLYPFPQEEYEAILRKY
                     ENAREIVWCQEEPQNQGGWYQIRHRLQSKLGPNDELLYAGRAGAAAPATGIHGLHDEQ
                     QKNLVAAALQGLPPEEASRHTMRLPVIQIRTG"
BASE COUNT          338 a          600 c          579 g          388 t
ORIGIN      
        1 tccttggcgg acaggtaatt cacgacatcc gacttcgaga cacggccgtc gcggcccgag
       61 ccggtcactg ccttcggatc gacctggttc tcttcgagga cgcgttttgc ggccgggctg
      121 agtttgcccg caaccgattc ggtcgactcc ttggcggtcg tcgcggcgct gtcggacgcc
      181 atgatggccg gcgcactggc acccgggagt gcggtggcgc cctcctcgag tatggccagc
      241 acctgaccgc tggtcaccga ggtgccatcc ttaagcgtga tttccttgag cacgccggcc
      301 gccggtgccg ggacctcgag aaccaccttg tcggtttcca agtcgacgag attttcgtcg
      361 cgcgccaccc aatcgccggg ttttttgtgc caagacacaa tggtagcgtc agccaccgac
      421 tcgggtagct gcggcacgcg aacttcaata gtcattaacc tgtcctaatt tgaatcactg
      481 gaaggcgcat cgtgtgccgg gaggcctctt ccggtggaag gccctgcagc gccgcggcga
      541 ccagattttt ctgttgctcg tcgtgcagac catggatgcc ggttgccggc gccgcggcgc
      601 cggcgcgtcc cgcgtagagg agttcgtcgt tcggacccag tttcgattgc aatcgatgcc
      661 ggatttgata ccagccgcct tggttttgcg gctcctcctg gcaccagacg atctcgcggg
      721 cattttcgta cttgcgaaga atcgcctcat attcttcctg cgggaagggg tagagttgct
      781 cgagccgcac gatggcaaca gtgtccactt tgacttcgcg ccgtgctttc agaaggtcga
      841 aatatacctt gccgctgcac agcaccaggc gggtcacagc cgcgggtttg atgtcgtcga
      901 cttcatcgat taccgagtag aagccgccgc tgctcagctc ttcgagccgc gacaccgata
      961 acgggtgtcg cagcaagctc ttcggcgtca tgacgatcag cggcttgcgc aggtggcgca
     1021 ccatctgccg gcgcaacatg tgaaacattt gcgccggagt cgagggcaca cacacctgca
     1081 tgttgaactc ggcacagagc tgcaggaaac gctcgagacg tgctgacgaa tgctcggccc
     1141 cttgaccctc gtagccatgc ggcaagaaca aggtgagccc cgacagccgg ccccatttgg
     1201 cctcgccgga cgtgatgaat tgatcgatga tgacctgcgc cccgttgcag aagtcgccga
     1261 actgcgcctc ccaaatagtc aagcaatgcg gttcggtggt cgagaaacca tattcgaaac
     1321 ccaacaccgc ttcctcggag aggaccgaat cggtgacggt gaacgtcggc tggttggtcg
     1381 ccaaatgttg cagcggggta tacaggcggc cggtgacctg gtcgtgcagc accgcgtgac
     1441 ggtgaaagaa tgtgccgcgc ccgctatcct ggccggtcag gcgtaccgga tagccttcct
     1501 gcacgaggct ggcatacgcc agattttcgg cagcacccca gtcgagcgcg aggtcgccgt
     1561 ttaccattcg ttcgcgtgcc tgcatgatgg cgagaacgcg ggggtgcaga acccagtccg
     1621 acggataggt cgtgatcgcc ttgccaaggg cgcgtaagcg ccccatgtcg atcgcggtct
     1681 tcaccggctc tccccagtcc gcgcccaagt actcgctcca atccaccgtg aatttgttgc
     1741 cgatgagacc gagggcggcg cgcgcctgcg gtttgccctc atcgaggcca ttgcggtact
     1801 gctcgatcat ggccgtcgcc tccgcggcgc tcagcacgcc ctcggccgcg agtttttccg
     1861 cgtacaactg acgcgccgtc ggtttcttgc gaatcacctg ataca
//
LOCUS       DASWZT010000448         1518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_95609, whole genome shotgun sequence.
ACCESSION   DASWZT010000448 DASWZT010000000
VERSION     DASWZT010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95609"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="VGE92_12725"
     CDS             <1..1029
                     /locus_tag="VGE92_12725"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY1314738.1"
                     /translation="VHNIQPGDVVAHAAPMSHGSGIVALPAVARAATNAILISKSFEP
                     KALFALIEAMKVSHIAFLAPTQIIKMLEEFVPGSYDLSSLKAICYGGAPIYVEHLRNA
                     IKAFGPVFAQIYGQGEAPITISGLNAAEHARLLAANDPRIGSAGTIRTDVEVRCVDAD
                     DRPLPCGQEGEIVVRGDVVMPGYWNNPAATAETLRGGWLHTGDIGLLDTTGYLFLLDR
                     AKDMIISGGNNVYPREVEEVIVRHPAVATAVVFGIPHAYWGEAVHALVVLESGATATA
                     QDIIDHCGRNMAGYKKPKSVEFVDSVPVSGYGKVMRREIREKYWQGYESRVGGGAPKS
                     VTLNEERQ"
     gene            1026..>1518
                     /locus_tag="VGE92_12730"
     CDS             1026..>1518
                     /locus_tag="VGE92_12730"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEY1314739.1"
                     /translation="MSTVLIAGIGMTPFGRFPGRGIRTLAVAAIDEALADAGIPAEHV
                     DKVFFGNAVAGIVTHQEMIRGQVALRHHPLARSSVINVENACASGGSALNLGFEAIAG
                     GRAEVVLVVGVEQLNHEDRSRPFDALRGSTDIEDIGEGVPGQIAANSILMDFYAGVAR
                     AYLE"
BASE COUNT          281 a          475 c          491 g          271 t
ORIGIN      
        1 gtacacaaca tccagccggg cgacgttgtg gctcatgcag caccgatgtc gcacggcagc
       61 ggcatcgtcg cgctgccggc cgtggcgcgc gccgccacca atgccatcct catcagcaag
      121 agtttcgagc ctaaggcgct gttcgcgctg atcgaggcga tgaaggtctc gcacattgca
      181 tttttggcgc ccacccagat catcaaaatg ctcgaagagt tcgtgcccgg ctcgtatgac
      241 ctatcgagcc tcaaggccat ctgctacggc ggcgcgccaa tctacgtcga acacctgcgc
      301 aacgcgatca aagcgttcgg tcccgtgttt gcgcagatct acggtcaagg cgaggcaccc
      361 atcaccatca gcggactcaa cgcagccgag catgcccggc tgctcgccgc caatgatccg
      421 cgcataggct cggcggggac gatccgcacc gacgtcgagg tgcgctgcgt cgacgccgac
      481 gatcgcccgc tgccctgtgg gcaggagggt gagatcgtgg tgcgcggcga cgtagtcatg
      541 ccggggtact ggaacaatcc cgccgcgacg gcggagacgc tgcgcggcgg ctggctgcac
      601 accggcgaca tcggcctgct cgacaccacg ggctatctgt tcctcctgga ccgtgccaag
      661 gacatgatca tcagcggcgg caacaacgtc tacccgcgcg aagttgagga ggtcatcgtc
      721 cggcaccctg ccgttgcgac ggccgtcgtg ttcgggattc cgcatgctta ttggggcgag
      781 gcggtgcacg cgttggtcgt gctcgaatcg ggagccaccg cgaccgctca agacatcatc
      841 gaccactgcg gccggaacat ggcgggatac aagaaaccca agagcgtgga attcgtcgat
      901 tcggtcccgg tgagtggcta cggcaaggtc atgcggcgcg agatccgcga aaagtattgg
      961 caaggttatg aatctcgcgt cgggggcggt gcccccaaat cggtcacact caacgaggag
     1021 cgccagtgag cacagtctta atcgccggta tcggcatgac gcccttcggt cgttttccgg
     1081 gacgcggcat tcgtaccctg gcggtggccg ccatcgatga ggccttggcc gacgcgggca
     1141 tccccgccga acatgtcgac aaagttttct tcggcaatgc ggtggccggc atcgtcactc
     1201 accaggagat gatccgcggt caggtcgcgc tgcggcatca tccgcttgca cgctcatcgg
     1261 tgatcaacgt cgagaacgca tgcgcctcgg gaggctcggc gctcaatctt gggttcgagg
     1321 cgatcgccgg aggtcgcgcg gaggttgtgc tggtcgtggg tgtcgaacag ctcaatcacg
     1381 aggatcgttc gcggccattc gacgcactgc gcgggtcgac ggacatcgaa gacatcggtg
     1441 agggggttcc cggacaaatc gcggccaact ccatcctgat ggatttctac gccggcgtgg
     1501 cgcgcgccta tctggaga
//
LOCUS       DASWZT010000449         3153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_71288, whole genome shotgun sequence.
ACCESSION   DASWZT010000449 DASWZT010000000
VERSION     DASWZT010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71288"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VGE92_12735"
     CDS             <1..688
                     /locus_tag="VGE92_12735"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY1314740.1"
                     /translation="FSAYDPRQPTQEALERTVRLTRQVREAVGRRADLLVGTHGQFTT
                     SGAIRLAKHLEPFDPLWFEEPVPPEMPEQMAVVARATSIPIATGERLSTKYEFARVLA
                     CGAANILQMNLGRVGGLLEAKKVAALAEVHYAQIAPHLYCGPVVGAANIQIATCSANF
                     LILESIQQWGGIHADLLLRPIRWEQGYVIPPTAPGLGVELNEAVALAHAYLEDTPLHL
                     EMIDRPLGNP"
     gene            699..1616
                     /locus_tag="VGE92_12740"
     CDS             699..1616
                     /locus_tag="VGE92_12740"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY1314741.1"
                     /translation="MTLRYGFIGLGNLGKHLAANLARGGFEVLVYDLQRSGAEPALRA
                     GARWVDSVADLARSCDGLITCLPSPAASAAVLDLALPALRPGSTWIEMSTNDFAEVER
                     LAARAHGLGIATLACPVTGGVHRAAAGDITVLVGGAQQVFARHEPALRAMGGRVIHLG
                     GIEQAAVTKVVTNMLAFIHLIAAGEALMLCAKAGVDLGSAYAAIAASSGNSFVHETES
                     QVILNGSYDIGFTMDLACKDAGFAVEFGRRFGVPLRLAAIMEQTFIEGRAKYGGSAWS
                     SMIVKLLEDAVGTPLRAPGFPTQLGGDGA"
     gene            1767..>3153
                     /locus_tag="VGE92_12745"
     CDS             1767..>3153
                     /locus_tag="VGE92_12745"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY1314742.1"
                     /translation="MNSNQKLSYAIAAILSGAPAGLLHAAPAAATDTDTSADSIQEIT
                     VTAQRRSESIQNVPITIQALTAETLKELSVATFDDYVRYLPNVTVASNGPGQGDIYMR
                     GLSVGNAGSQSSGTIGGFPNVAIYLDEQSGQLPARNLDVYAADLERIEVLEGPQGTLF
                     GAGAEAGVIRYITNKPKIDVTEGNVDAGYGITAHGDPNTNLSAVLNLPLIANTLAVRA
                     VIYNDSRGGYIDNVPGTFTRQATDVGIGYAHNPGGGVPANSTVINNNSVVGRAINPVT
                     YTGGRAEALLKINDDWDILLSQSYQNMDSEGVFYQMPYSSDGVKLAPLEVTLFNDSYD
                     KDRFENTAWTVNGKLGDLKLVYTGGYLVRHVSQVADYTNYARGAYADYYQCFGPTKSQ
                     PTLTSTCYSPSSTWRETERNEHLSNEVRLSTPDDWRIRGIIGVFYETNKLFDQTDWYY
                     KTLPDCTSNTAP"
BASE COUNT          621 a          993 c          962 g          577 t
ORIGIN      
        1 gttctcggcc tacgatccgc ggcaacccac gcaggaggca ctcgaacgga ccgtgcgcct
       61 gacccgtcag gtgcgcgagg ccgtggggcg tcgcgccgat ctgttggtgg gcacccatgg
      121 ccaattcacc acctcgggcg ccatccgcct cgccaagcac cttgaaccct tcgatccgct
      181 ctggttcgag gagccggtac cgccggagat gcccgagcag atggccgtcg tcgcccgggc
      241 aaccagcatt cccattgcca ccggtgaacg gctgagcacc aaatatgagt tcgcgcgcgt
      301 cctcgcctgc ggcgcggcga acatcctgca aatgaacttg ggccgcgtcg gaggattgct
      361 cgaggcaaaa aaagtcgcag cgctggcgga ggtccattat gcgcaaatcg cgccgcatct
      421 ttattgcggg ccggtggtgg gcgccgccaa catccagatc gcgacgtgct ctgcgaattt
      481 tctgattctc gaaagcatcc agcaatgggg cgggatccac gccgatctct tgctgcggcc
      541 gatccgctgg gagcaaggtt acgtgattcc gccgacggcg cccgggctcg gggtggaact
      601 gaacgaagcg gtggccctcg cccacgctta cctcgaagac acgcccttgc acctggaaat
      661 gatcgaccgc ccgctgggaa atccttagag tccgttccat gacgctgcgc tacggattca
      721 ttggcctcgg caatctcggt aagcacctgg ccgccaatct ggcgcgcggc gggttcgagg
      781 tcctggtgta tgatttgcag cgcagcggag ccgagcctgc gctgcgcgcc ggagcccgct
      841 gggtggattc cgtcgccgac cttgcgcgca gctgcgacgg cttgatcacc tgcctcccct
      901 cgcccgccgc cagcgccgcg gtgctggatc ttgcattgcc ggcgctgcgg ccgggcagca
      961 cctggatcga gatgagcacc aatgacttcg ccgaggtgga aaggctcgcc gcgcgggccc
     1021 acgggttggg cattgccacg ttggcctgtc cggtgaccgg gggtgtgcac cgcgcggcgg
     1081 ccggcgacat caccgttttg gttggcggcg cgcaacaagt attcgcaagg catgagccgg
     1141 cactgcgggc gatgggtggc cgggtcatcc atttgggagg aatcgagcag gccgcggtga
     1201 ccaaggtcgt caccaatatg ctggccttca tccacctgat cgcggccggc gaggcgctga
     1261 tgctgtgcgc caaagcgggt gtcgacctag gctcggcgta tgcggcaatt gccgcgagtt
     1321 cgggcaatag cttcgttcac gagaccgaga gccaggtcat tttgaatggc agctatgaca
     1381 ttggttttac aatggattta gcctgcaagg acgccggatt cgcggtcgaa ttcggacgtc
     1441 gcttcggggt accgctgcgg cttgcggcga tcatggaaca aaccttcatc gagggtcgcg
     1501 ccaagtatgg cggcagcgcc tggtcgtcca tgatcgtcaa attgctggag gacgcggtcg
     1561 gcacgccgct gcgtgctccc gggtttccca cgcagctagg gggcgacggc gcttgaaatc
     1621 gaattttcgc atgcccaaat cgaaaatcgg catatgcccg atgtatgccc acaacaaaat
     1681 agcgcttccg ctttgccggg gcgatgctat tctctgaatc attacaaatg acggtcaccg
     1741 tcatcatata gggaagggat cgacgcatga attcaaatca gaagttatcc tatgcgattg
     1801 ccgcaattct gagcggcgcc cccgctgggc tcctccacgc cgccccggct gcggccaccg
     1861 acacggatac ctcggccgac tcgattcaag agatcaccgt gaccgctcag cggcgctcgg
     1921 aaagcattca aaacgtgccg atcaccatcc aggcgctgac ggcggagacc ttgaaggaat
     1981 tgagcgtcgc gaccttcgat gactatgtgc ggtatttgcc caatgtgacg gtggcttcca
     2041 acgggccggg ccagggcgac atctacatgc gcggcttgag cgtcggtaac gcgggcagcc
     2101 agtcgagcgg cacgatcggg ggatttccga acgtcgcgat ctacttggac gaacaatccg
     2161 gtcagctgcc ggcgcgaaac ctggatgtct acgcggccga cctcgagcgc atcgaggttc
     2221 tcgaaggccc acaaggcacc ctgttcggcg ccggtgccga ggcaggcgtg atccgctaca
     2281 ttaccaacaa gccgaagatc gacgtgaccg aaggcaacgt tgatgccggc tacggcatca
     2341 cggcgcacgg cgacccgaat accaacttat cggccgttct caatttgccg ctgatcgcca
     2401 atacgctcgc cgtgcgtgcg gtgatctaca acgatagccg cggtggttac atcgacaacg
     2461 tgccgggaac gttcacccgt caagcgaccg acgtcggtat cggctatgcc cataatccgg
     2521 gcggcggtgt gccggcgaat tcaacggtca tcaacaacaa ttcagtggtt ggacgcgcga
     2581 tcaatccggt cacttacacc ggcggccgtg ccgaagcgct gctcaagatc aacgacgact
     2641 gggatatcct gctgtcgcag tcctaccaaa acatggactc ggaaggcgtt ttctatcaga
     2701 tgccctactc ctccgacggc gtgaagctcg ctccgctcga agtcacgctg ttcaacgact
     2761 cctacgacaa ggacagattc gagaacactg cgtggaccgt gaacggcaag ctcggggact
     2821 tgaaactcgt ctacaccggc ggctatctgg tgcgccacgt cagtcaggtc gccgactaca
     2881 cgaactacgc gcgcggcgct tacgccgact actaccagtg ctttgggccc accaaatctc
     2941 aacccacgct cacctctacc tgctactcgc cgagcagcac gtggcgggaa accgaacgca
     3001 acgagcacct gagcaacgag gttcgcctga gcacgccgga tgactggcga atccgcggca
     3061 tcatcggcgt tttctacgaa acgaataagc tcttcgacca gaccgactgg tactacaaga
     3121 cgctgcccga ctgtacctcg aacaccgcac cgg
//
LOCUS       DASWZT010000450        11823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_56782, whole genome shotgun sequence.
ACCESSION   DASWZT010000450 DASWZT010000000
VERSION     DASWZT010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11823
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56782"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="VGE92_12750"
     CDS             <1..976
                     /locus_tag="VGE92_12750"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY1314743.1"
                     /translation="HLRAADAAPPSGAVLLGARASSAASQASAGTSDDSSWLARETVK
                     LVKAISAPHLQPRVALLALLGALLLGAVHALSPGHGKTIVGAYLIGSRGTPRHAAFLG
                     FTVTVTHTLGVFVLGFATLYASRYIVPERLFPILSLVSALLVLGMGLALLMQRVRAAR
                     TAAGPGVPAVNTPVFHPIEPVHGALLRRDLILAHSHGDGTWHSHGGKMHTHLPPGAAG
                     ERVTWRSLLALGVSGGLVPCPSAMVLLLAAVALNKTAYGMLLVLTFSVGLAITLTLVG
                     LVFLHARNRLRPSSGSGRWMHLLPVLSAVTITIVGVALCCGALRSFNW"
     gene            complement(993..4577)
                     /locus_tag="VGE92_12755"
     CDS             complement(993..4577)
                     /locus_tag="VGE92_12755"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY1314744.1"
                     /translation="MSRDVAAAIRSEATQSADGLNHLLERQIAETRSRGGEIDVAALL
                     KAVSAHYDRIDGERRGIVRSMQLMSDEAQALTREIREQTASHLQSIMDNVKDAIVTVD
                     DAGHIETFNPTGERIFGYASAEILGRSLDFLLPDIANGTSDYLERLATKIDDTHVDLA
                     AHQTWGRAKGGNRVAVEIAISKAKLNWRDAYIVSIRDITERHLAEQRVRESEARYRTL
                     VEHAPEVIVVFDVDQGKFVDVNDNACRFFKMSRETLLASGPDKISPAQQPDGASFGRP
                     RGFIEGALAGDAPVFEWMHCDSTGQSIPCEVRFVRLPSSNRRLIRASITDITERKRAD
                     AIAAGERRVFEKIAANAPLTSALESICELIERVVIESHCAINLLDQDRKALNFGVAPN
                     LPREFVAAMDNAPVGIRYGSCAAAVYLARQISVADIRTDALWEYRREAALLASLRAAW
                     STPIIASDGRIVGTFAVYRRHPGIPLARDHELMLRMAQIAGIAIERRGAEEALRNSEA
                     KFRGLFESMMEGVYKTSRDGRILVANPAFVNLMGYESAEELYQVPAAALYWYPSDRDT
                     FVRRVESEGELRNEEYVLRRKDGSMLVVMDSSRVVRDEQGRVTGYEGTLTDITERKKA
                     ETAVFQAKERAQVTLQSIGEAVITTDSEGRIDYMNPVAESLTGWENREAQSQLIGEVL
                     TVVDEGTREASESPVMRCLREGQILGLTEHTVLVNRRGQELAIQDSAAPIRDRAGNLI
                     GAVMVFHDVSKERRLHRALHYQASHDALTGLINRREFEIRLTAAVENSRQDSGGRHAL
                     LYVDLDQFKLVNDTCGHPAGDQLLKQITAVLQSRVRSGDTLARLGGDEFGILLQSCSL
                     DQALRIAEGLRQAIRDFRFIWQDGVLEVGASIGVVEITNETPTVANVMSAADVACYSA
                     KDLGRNRVQLYKPDDVPERHREMHWVSKLARACDESRFELFYQPIVPIGATLHEREHF
                     ELMLRLRDESGALVTPAEFIPAAERYNVMPSIDRWVVRQALDRLVHRIGSGVKPFTVA
                     VNLSGTSLNDERFLEYLIAELSHNELNAGAVCFEITETAAIANLANVVYFMRELKARG
                     CHFALDDFGSGLSSFMYLKTLPVDYLKIDGQFVENVTRDRVDRSMVEAISQVGKTMGI
                     QTIAERVESAEVLAELGRLGVGYAQGFHIAPPRTTLEFPYLREPIRKS"
     gene            complement(4722..6551)
                     /gene="typA"
                     /locus_tag="VGE92_12760"
     CDS             complement(4722..6551)
                     /gene="typA"
                     /locus_tag="VGE92_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEY1314745.1"
                     /translation="MTQKLRNIAIIAHVDHGKTTLVDQLLRQSGTLDARKDLQERVMD
                     SNDLERERGITILAKNTAITWGDYRINIVDTPGHADFGGEVERVLAMVDCVLLLVDAV
                     DGPMPQTRFVTQKAFKHGLRPIVVINKIDRDEARPGWVLDQTFDLFDRLGATDEQLDF
                     PVVYASALRGFAGLESTVRDGDMQPLFKTIVEHCPPPDVDADGPLQLQVTLLDYSSYV
                     GAIGIGRIKRGTIKRGMAVTVVGREGKQRSERITQILGFHGLTRVEVESASAGDIVAF
                     AGIAEPKVSDTLCDPAKVEPLPSLTVDEPTISMTFEVNTSPFLGRDGKFVTSRQIRER
                     LEREAIHNVALRVEPTADPDKFVVFGRGELHLGVLLENMRREGYEMAVSRPRVVIKQV
                     DGAAHEPYEQVTVDADADAQGDIMSALGSRGAELKDMQADGRGRIRLDYLVPSRGLIG
                     FQTEFRTMTRGTGLLHHVFDHYGPVVQRELGQRANGVLIANGQGIALAYSLFSLQERG
                     RLCIGPGEEVYEGMIIGLNNRDNDLVVNPLKGKKLTNMRAAGKDENVVLTPPIRFSLE
                     QAMEFIDDDELIEVTPNSIRLRKRSLRENDRKRSEREAVDQAS"
     gene            complement(6610..7107)
                     /locus_tag="VGE92_12765"
     CDS             complement(6610..7107)
                     /locus_tag="VGE92_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314746.1"
                     /translation="MHTARVLTLCSVAAVSACHGDSPPPAAKPHFTLPAAAPKGPSAE
                     ELTRGMVLAASLGKSQLPVDLKFDLRQRPIVGQALDVDIALMPQIDASPADIQVTGGY
                     GLSVAPGTDQIDLPAVQAGQAYRQSFKVTPSMDGVLMLGLSVSLKHDDVTESRVFSIP
                     LIVER"
     gene            complement(7137..8117)
                     /locus_tag="VGE92_12770"
     CDS             complement(7137..8117)
                     /locus_tag="VGE92_12770"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEY1314747.1"
                     /translation="MNSDAPYSALSPEKILDAIEAVGYRCDGRVLALNSYENRVYQIG
                     IEEGAPLIAKFYRPARWSDAAIREEHAFAGELAAQEIPVVAPLLRDGNSLHVHLGFRY
                     AVFPRRGGRWPELGVSDDREWVGRFLGRIHAVGRAARFYERTRLSMEDLGRRSRDFIL
                     EGDWMPDYLATKYADVTDELLYEVEARADGWGGAAVGRILGDCHRGNILWTEQGPHFV
                     DLDDCLTGPAIQDLWMLLSGGQQEMRTELHDLLKGYEQFLAFDRSEIALIEPLRALRM
                     IHYSGWLARRWHDPAFPQAFPWFAEPRYWEQHYRALQDQLAAVLAPPLEL"
     gene            8218..9642
                     /locus_tag="VGE92_12775"
     CDS             8218..9642
                     /locus_tag="VGE92_12775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY1314748.1"
                     /translation="MLIGFTIVAAPLLFAIVNAAVQMNRLSARSEQLVLHGVRGTRNN
                     QRMFEEISALERTARLYQIIGNADLLDVYARNQERLLATLNEMRSMPLDAESHGEAQA
                     LQAESARLQQQLKSSAPNSARMADLINAFPQLSEKASNVSEHVSAQIDRELSSLQLAT
                     QLAQRNLFWETLLLVPLTLAVVGVFTYLFGRPIRAIDRAISELGRGTFSRPIAIRGPA
                     DLERLAAQLEWLRGRLLDLAQEKNRFLRHMSHELKTPLANIREGTELLMDGAVGELQS
                     GQREVTAILRENGMKLQRLIENLLSFSAWQAKSVGLEITEFKLRPLVKSVLENQQLTL
                     VAQRVRLDVHIEDLTPVADRGKVRLILDNLLSNAIKFTPRGGTISIHARGEREQLVID
                     VIDSGPGIPPDDRKRIFEAFYQGKTPQGGHVKGTGIGLSVVTEFVNAHAGSVEILEAK
                     SGGAQFRVRLPMRQAVPREKAHAA"
     gene            9620..10261
                     /locus_tag="VGE92_12780"
     CDS             9620..10261
                     /locus_tag="VGE92_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314749.1"
                     /translation="MKKLMRHRWLRIAACAFAIGSVCGCGVEGSFARQKPVAPAQPDP
                     AQDLSPITPLLQMMSSLPQGDPGRQAEIFQSAKDAADLAPTTSNRLRYALALATPGFS
                     GTDDVAAQRQLAELLARPETLLPIERLLALVELKEVEQRLILQAENKRVRDDAQHDDS
                     RDKLATANRRLAAESDENVRLRKALDEARAKLEAVTHIERSINDRGTSGSHTP"
     gene            10282..11694
                     /locus_tag="VGE92_12785"
     CDS             10282..11694
                     /locus_tag="VGE92_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013358747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY1314750.1"
                     /translation="MNAYSIAATTPLSFGLHPGARETQTPQKRKARILVVDDDPGLLR
                     LLTIRLRAENYEVEAVESAAAALAACLRFRPDLVITDLRMDQMDGIGLLKELQSRWPG
                     LRVIILTAHGTIPDAVHATQSGAFGFLTKPVDKQDLLDHVQKALKVSGFAHVDEDWRS
                     NIITRNQLMEDKLAQANMVAGTDARVLLTGESGTGKELLARAIHEASPRRNKPFIAIN
                     CSAMAENLLESELFGHEKGAFAGATRSQKGLFETADGGTLLLDEVGDMPMRLQVKLLR
                     VLQENQIRPVGAAETVPINVRIISATHRDLHELMVGGQFREDLYYRLNVVHIEMPGLG
                     RRREDIPLLVAHFLEQIAKESGQRKIYAPEAVELLATAEWPGNVRQLYNVVRQNVALS
                     HGAIITAELVQQSLGGNPTRLPSFDEARDEFTRNYLSQILQITGGNVSQAARLAKRNR
                     TDFYKLLSRHQLLPDDFKKS"
BASE COUNT         2217 a         3694 c         3624 g         2288 t
ORIGIN      
        1 gcatttgcgc gcggccgatg cagcgccgcc gtcgggtgcg gtgctgttgg gtgcgcgtgc
       61 atcgagtgcg gcgtcccaag catccgccgg cacgagcgat gacagcagct ggcttgcgcg
      121 cgagaccgtc aagctggtga aagcaatttc agcgccgcat ttgcaaccgc gggttgcctt
      181 gctggcgctt ctcggtgcgt tgctgctcgg cgccgtccac gcattgtcac ccggacacgg
      241 aaaaaccatc gtcggtgcgt atttgattgg atcgcggggt actccgcggc acgccgcatt
      301 tctcggattc accgttaccg tgacgcatac cctaggcgtg ttcgtgttgg gatttgccac
      361 cctgtatgct tcgcgctaca tcgtgcccga gcggctgttt ccgatcttga gcctcgtctc
      421 ggcgctgctg gtgctcggca tgggtctggc attgttgatg caacgagtgc gcgcggcgcg
      481 caccgcggct gggcctggcg tacctgcagt caataccccg gtgttccatc ccatcgagcc
      541 ggtccacggc gcactcttga ggcgcgactt gatacttgcc cacagccacg gcgacggcac
      601 atggcattcg catggcggga aaatgcacac gcacctgcca ccgggcgccg cgggtgagcg
      661 tgttacttgg cgcagcttgc tggcgttggg cgtttccggc ggcttggttc cctgtccgtc
      721 cgccatggtg ctgctgttgg ccgcggtcgc actcaataag actgcttacg gcatgctgtt
      781 ggtgttgacc ttcagtgtcg ggctcgcgat caccttgacg ctggtcggcc tggtgttcct
      841 gcacgcgcga aatcggctgc gtccgtcgag cggcagcgga cgctggatgc atctattgcc
      901 ggttttgagt gcggtgacga ttactatcgt cggcgtggcg ctgtgttgcg gcgcgctgcg
      961 cagcttcaac tggtagtgcg ttccaccaat gtttatgact tcctgatcgg ctcgcgcagg
     1021 tagggaaatt ccaacgtcgt gcggggaggc gcgatgtgaa atccctgggc gtagcccacc
     1081 ccgagccgcc cgagttcggc caggacctcg gcagactcca cgcgttcggc gatggtttgg
     1141 atgcccatgg ttttgccgac ctggctgatg gcctcgacca tgctgcgatc taccctgtcg
     1201 cgcgtgacat tttcgacgaa ctgtccgtcg atcttcaaat aatcgacggg cagggtcttc
     1261 aagtacatga acgaagacag accgctgccg aagtcgtcca gcgcaaagtg gcagccgcgt
     1321 gctttgagct cgcgcatgaa atagaccacg ttggccaagt tggcgatggc ggcggtttcc
     1381 gtgatctcga aacaaacggc cccggcgttc aattcattgt ggctgagctc ggcgatcaag
     1441 tactcgagaa agcgctcatc gttgagcgag gtgcccgaga gattgacggc aacggtgaac
     1501 ggtttcaccc ccgacccgat gcgatgcacg agccggtcga gagcctggcg gaccacccag
     1561 cggtcgatcg acggcatgac gttgtagcgc tccgccgccg gtatgaattc cgcgggggtc
     1621 accaaggcgc ccgattcgtc ccgcaagcgc agcatcagtt cgaagtgttc gcgctcatga
     1681 agggtcgcgc cgatgggcac gatcggctgg taaaacaact cgaaacggct ttcgtcgcag
     1741 gcgcgtgcca actttgagac ccagtgcatt tcgcgatgcc gctccggcac gtcgtcgggc
     1801 ttgtagagtt gcacgcgatt gcggcccagg tccttcgctg aatagcatgc aacgtcggcc
     1861 gcgctcatga cgttggcgac tgtcggcgtc tcgttggtga tctcgacgac accgatgctc
     1921 gcgccgacct cgagcacgcc gtcctgccag atgaatcgaa aatcacggat cgcctggcgc
     1981 agaccctcgg cgatgcgcag cgcctgatca agcgaacagc tctgcagcaa aattccaaac
     2041 tcgtccccgc cgagacgcgc caacgtatcg ccgctgcgca ctcgcgactg cagcaccgcg
     2101 gtgatctgct tcagcagctg atcgcccgcg ggatggccgc aggtgtcgtt gaccagtttg
     2161 aattgatcga ggtcgacata cagaagcgcg tgccggccgc cgctgtcctg gcgcgaattc
     2221 tcgaccgcgg ccgtcaggcg aatctcgaac tcgcggcggt tgatcaatcc ggtcaacgca
     2281 tcgtggctcg cctgatagtg cagtgcgcgg tgcagacgcc gttccttgct gacgtcgtgg
     2341 aagaccatga cggcgccgat gagattgccg gcgcgatcgc ggatcggcgc cgccgaatct
     2401 tgaatggcca gttcctggcc gcggcgattc accagcacgg tgtgctcggt gagtcccaga
     2461 atctgtccct cgcgcagaca gcgcatgacc ggactttcgc tggcctcgcg agtgccctca
     2521 tcgacgacgg tcaacacctc gccgatgagc tgcgattgcg cctcgcgatt ttcccagccg
     2581 gtcaagcttt cggcgaccgg attcatgtaa tcgatgcgcc cttcggagtc ggtggttatg
     2641 acggcctcgc cgatcgattg caaggtaacc tgggcacgct ctttggcctg gaatacggcg
     2701 gtttccgctt tcttgcgctc cgtgatatca gtcagcgtac cctcgtatcc cgtcacccgc
     2761 ccctgctcgt cgcgtaccac ccggctcgaa tccatgacca ccagcatgga gccgtccttg
     2821 cggcgcagca cgtactcctc gttgcgtagt tcgccttcgc tctcgacgcg cctgacgaag
     2881 gtgtcgcggt cgcttgggta ccaatacagc gctgcggccg gcacttgata cagttcctcg
     2941 gctgattcat agcccatcaa gttcacgaat gccggattgg ccaccagaat tcggccgtcg
     3001 cgcgacgtct tgtaaacccc ctccatcatg ctttcgaaca gaccgcgaaa tttggcttca
     3061 ctgttgcgca gcgcctcctc ggcaccgcgc cgctcgatgg cgatgccggc gatttgcgcc
     3121 atgcgcagca tcaattcgtg atcgcgcgcc aacggaatac ccggatggcg cctataaacc
     3181 gcgaaagtac cgacgatgcg cccgtccgag gcgatgatgg gagtggacca cgcggctcgc
     3241 aacgaggcca agagtgccgc ctcacgccga tattcccaca gcgcatccgt gcgaatgtcg
     3301 gcgacgctga tctggcgcgc caaatacacg gcggcggcgc acgatccata gcggatgccg
     3361 accggcgcat tgtccatcgc cgccacgaac tcgcgcggca agttgggcgc cacgccgaaa
     3421 ttcagagcct tgcgatcttg atcgagcagg ttgatggcgc agtggctctc gatcaccacg
     3481 cgctcgatca gctcgcaaat cgattcgagc gccgaagtca gcggcgcgtt ggccgcgatc
     3541 ttctcgaata cgcggcgttc gccggccgcg atcgcatcgg cgcgcttgcg ctcggtgata
     3601 tcggtgatgc tggcgcgtat cagccgtcga ttggaactcg gcagacgcac gaagcgcact
     3661 tcgcaaggaa tgctttgccc ggtcgagtcg cagtgcatcc attcgaagac cggtgcatcg
     3721 cctgccagcg cgccttctat gaagccgcgc ggccgcccaa aggacgcgcc gtcgggttgt
     3781 tgcgccgggc tgatcttgtc cgggccgctg gcgagcaagg tttcgcgcga catcttgaaa
     3841 aaacggcagg cattatcgtt gacgtcgacg aattttccct gatcgacgtc gaacacgacg
     3901 atcacttccg gtgcatgttc caccagcgtt cggtagcgcg cctcgctttc gcgaacccgt
     3961 tgctcggcaa gatgccgttc ggtgatgtcg cggatgctga cgatgtaggc gtcgcgccag
     4021 ttgagcttgg ctttgctgat ggctatctcg accgcgacgc gattaccgcc cttggcccgt
     4081 ccccaggtct ggtgtgccgc caggtccacg tgcgtgtcgt cgatcttggt ggcgaggcgc
     4141 tcgagataat cgctggtgcc gttcgcgatg tcgggcagca ggaaatcgag cgagcgcccg
     4201 agaatctctg ccgacgcgta gccaaaaatg cgttcgcccg tgggattgaa ggtttcgata
     4261 tggccggcgt cgtccaccgt gacgatagca tccttgacgt tgtccatgat cgactgcagg
     4321 tgcgaagcag tctgttcgcg gatctcgcgc gtcagcgcct gcgcctcgtc ggacatgagc
     4381 tgcatcgagc gcacgatgcc gcgccgttca ccatcgatcc gatcgtagtg cgcgctgacc
     4441 gctttcagca gtgcagcgac atcgatctcg ccgccgcggc ttcgcgtttc ggcgatttgg
     4501 cgctcgagca ggtggttcag gccatccgca gactgcgttg cctcgctacg aattgcagcc
     4561 gcgacgtcgc gtgacaagtc cctgatctcc aacgctgaat ttgcggcgcg cagtggcacc
     4621 ggaagtcagt agatgttagg gcgatcccgc aggcgcggcg tgacctgcgt ctcggtttgg
     4681 ccaacaatcc gttaaatatc agggcgtcgc cataccgggt atcaggaagc ctgatctacc
     4741 gcttcgcgct ccgagcgctt gcggtcgttt tcgcgcaaac tgcgcttgcg caagcgaatc
     4801 gagttcggcg tcacctcgat gagctcgtcg tcgtcgataa attccatcgc ctgctccagc
     4861 gagaagcgga tcggcggcgt gagcacgacg ttctcatcct tgccggcggc gcgcatgttg
     4921 gtgagtttct tgcccttgag agggttcacc acgagatcgt tgtcgcgatt gttgaggccg
     4981 atgatcatgc cttcgtaaac ttcctctccg ggaccgatgc acaaccggcc gcgctcctgc
     5041 aggctgaaca gcgaatacgc gagggcgatt ccctgaccgt tggcaatcag cacgccgttc
     5101 gcacgctggc ccaactcgcg ctgcacgacc ggtccgtagt gatcgaacac gtgatgcagc
     5161 aatcccgtgc cgcgggtcat cgtgcgaaac tcggtctgga agccgatcaa tccgcgcgac
     5221 ggcaccaggt agtccaagcg gattcggcca cggccgtcgg cctgcatgtc cttgagttcc
     5281 gcgccgcggc tgccgagcgc cgacatgatg tcgccctgcg cgtccgcgtc ggcgtcgacg
     5341 gttacctgct cgtacggttc gtgcgccgcg ccgtcgacct gcttgatgac tacgcgcggc
     5401 cgggaaaccg ccatttcgta gccttcgcgg cgcatgttct cgagcaatac tccgagatgc
     5461 aactcgccgc ggccgaagac cacgaacttg tcggggtccg cggtcggttc gacgcgcagc
     5521 gcgacgttgt gaatggcctc gcgctcgagg cgctcgcgaa tctgccggct ggtcacgaac
     5581 ttgccgtccc ggccgagaaa cggcgaggta ttgacctcga acgtcatgct gatggtgggt
     5641 tcatcgacgg tcaggctcgg cagcggctcg accttagccg gatcgcacaa agtatccgat
     5701 accttgggtt cggcgatccc ggcgaaggcc acgatgtcgc cggcacttgc cgactcgacc
     5761 tcgacgcgcg tcaatccgtg gaaccccaaa atctgcgtga ttctttcgga acgctgcttg
     5821 ccttcgcggc caaccacggt gaccgccata ccgcgtttga tcgtgccgcg cttgatgcgc
     5881 ccgatgccga ttgcgccgac gtagctcgag tagtccagca gcgtgacctg cagctgcaac
     5941 ggaccgtcgg catcgacatc gggtggcgga caatgttcca cgatggtctt gaatagcggc
     6001 tgcatgtcgc cgtcgcgcac cgtggactcg aggcccgcga agccgcgtaa cgccgaagcg
     6061 tacacaaccg ggaaatccag ctgttcatcg gtcgctccga gccgatcgaa caaatcgaag
     6121 gtttggtcga gcacccaacc cgggcgcgct tcatcgcgat cgatcttgtt gatgacgaca
     6181 atcggacgca ggccatgctt gaacgccttt tgcgtgacga accgcgtctg tggcatcgga
     6241 ccgtccaccg catccaccag cagcagcacg caatccacca ttgccaatac acgctcgact
     6301 tcgccgccga aatcggcgtg tccgggcgta tcgacgatgt tgatgcggta gtcgccccag
     6361 gtgatcgccg tattcttcgc gaggatggta atgccgcgct cgcgttccag atcattcgaa
     6421 tccatgacgc gctcttgcaa atccttgcgc gcatcgagcg tgccggattg gcgcaacaac
     6481 tgatcgacga gcgtggtctt gccgtggtcg acgtgcgcga tgatggcgat attgcgaagt
     6541 ttctgagtca cagtgaagcc ctggggaaac cgcccattat agagccttca ggcgacccgc
     6601 gccgcaattt caacgttcta cgatgagcgg aatcgaaaaa acacgggact cggtcacatc
     6661 atcgtgtttc agcgacacgc tcaagccgag catcaggacg ccatccatgc tcggtgtcac
     6721 cttgaagctt tggcgatacg cctggcccgc ctgaacggca ggcaaatcga tttgatcggt
     6781 gccgggtgca acgcttaaac catagccgcc ggtcacctgg atgtcggctg ggctcgcgtc
     6841 gatttgcggc atgagcgcaa tatcgacatc cagcgcctgc cccacgatcg gccgctgccg
     6901 caagtcgaat ttgaggtcca ccgggagctg cgatttacca agactcgccg ccaacaccat
     6961 ccctctcgtg agttcttcgg cactcggccc tttgggcgcg gccgccggta gcgtgaagtg
     7021 cggcttggcc gcaggtggcg gcgagtcccc atgacaggcg ctaaccgcgg cgacggagca
     7081 gagcgttaga actctcgcgg tatgcatccc gctatgatca ccaaatatgc cgccgatcac
     7141 aactcgagcg gcggtgcgag cacggccgcg agttgatcct ggagcgcccg gtagtgctgt
     7201 tcccaatagc gcggctcggc aaaccaggga aacgcctggg gaaaggcggg gtcgtgccag
     7261 cgtcgcgcca accaccccga ataatggatc attcgcaatg cgcgcagggg ctcgatcagg
     7321 gctatttcgc tgcgatcgaa cgccaggaat tgctcgtacc ccttgagcaa atcgtgcagc
     7381 tcggttcgca tttcctgttg gccgccggac aacagcatcc atagatcttg aatggcgggt
     7441 ccggtcaaac aatcgtccaa gtccacgaag tgcggtcctt gctccgtcca cagaatattg
     7501 ccgcggtggc aatcgcccaa aatgcggccg acggccgctc cgccccagcc atccgcacgc
     7561 gcctcaacct cgtacagcag ttcgtcggtc acatcggcgt atttggtcgc caggtaatcg
     7621 ggcatccagt cgccctcgag gatgaaatct cgcgaccgac ggcccaaatc ctccatcgac
     7681 aggcgtgtgc gctcgtagaa gcgcgcagcg cggcccaccg catggatacg gccaagaaat
     7741 cgccccaccc actcgcggtc atcgctcacc ccaagctcgg gccagcgtcc gccgcggcga
     7801 ggaaacacgg cataacgaaa cccgaggtgc acgtgcagcg aattgccgtc gcgtagcagc
     7861 ggcgccacca ctggaatttc ctgcgccgcc aactcgccgg cgaatgcgtg ctcttcgcga
     7921 atcgccgcat ccgaccatcg ggccggccga tagaatttgg cgatcagcgg cgcgccctcc
     7981 tcgatgccga tttgatacac cctattttcg tagctattaa gcgccaaaac ccggccatcg
     8041 caacggtaac cgacggcctc gatcgcatcc aaaattttct cgggggaaag cgcggaataa
     8101 ggtgcatcgc tgttcattcg aggaattcta caggtacgat gcagacactc ctttaagcgt
     8161 cacgctgcta gaatgccgcc catgcgattt cctcgaccta agtcattgag cgggctcatg
     8221 ctgatcggct tcacgatcgt cgccgccccg ttgctgtttg ccatcgtgaa cgccgccgtg
     8281 caaatgaatc gcttgtcggc gcgcagtgaa cagctggtgc tccacggcgt gcgcggtacc
     8341 cgcaacaatc agcgcatgtt cgaggaaatc agcgcgctcg agcgcaccgc gcgcctgtat
     8401 cagatcatcg gcaacgcgga tttgctcgat gtttacgccc gcaaccagga acggctgctg
     8461 gcgacgctga acgaaatgcg cagcatgccg ctcgatgccg agtcgcacgg cgaggcgcag
     8521 gctctgcagg ccgaatcggc acggctgcag cagcaattga agtccagcgc gcccaattcg
     8581 gcgcgcatgg cggacctcat caatgccttt ccgcagcttt cggagaaggc ctccaatgtg
     8641 tcggaacacg tcagcgcgca gattgaccgc gaactgagca gtctgcagct cgcgacacag
     8701 ctcgcgcagc gaaatctgtt ctgggagacc ttgctgctcg tgcccctgac gctggcggtg
     8761 gtcggggtgt tcacctattt gtttggccgg ccgattcgcg ccatcgaccg cgccatctcg
     8821 gagctgggtc gcggcacatt ttcccgaccc atcgctattc gcggaccggc ggacctggag
     8881 cgtctggccg cgcagttgga atggctgcgg ggccgtctgc tcgatctggc ccaggaaaag
     8941 aaccgatttc tccgccatat gtcacatgag ctcaagaccc cgctggccaa tatccgcgag
     9001 ggaaccgaac tcttgatgga cggcgcggtc ggtgagctgc aaagcggcca acgcgaagta
     9061 acggccatct tgcgcgagaa cggcatgaag ctgcagcggc tgatcgaaaa cctgctgtcg
     9121 ttcagcgctt ggcaggcgaa aagcgtcggc ctcgagatca cggaattcaa attgcgtccc
     9181 ttggtcaaaa gcgtgcttga gaatcagcag ctcacgttgg tggcgcagcg cgtgcgcctc
     9241 gatgtccata tcgaggatct gacgccggtc gcggaccgcg gcaaggtgcg tttgatcctc
     9301 gataatctgt tgtcgaacgc tattaagttc actcctcgcg gcggcacaat ctcgattcat
     9361 gcccgaggcg agcgcgaaca gctggtgatc gacgtcatcg acagcggacc cggaattccg
     9421 ccggacgacc gcaagcggat cttcgaagcc ttttatcagg gaaagacgcc gcagggagga
     9481 cacgttaaag ggacgggaat cgggctgtcg gtggtgaccg aattcgtcaa tgcacatgcg
     9541 ggcagtgttg agatcctcga ggccaagtcc ggaggcgccc agtttcgggt gcgcctgccc
     9601 atgcggcagg cggtgccccg tgaaaaagct catgcggcat agatggttgc gcattgcagc
     9661 atgcgcgttc gccatcggtt cggtgtgcgg ctgtggcgtc gagggctcat ttgcccgcca
     9721 aaagccggtg gcgccggcac agccggaccc ggcccaggat ttgagcccga taacgccgct
     9781 gttgcaaatg atgagcagcc tgccgcaggg cgatcctggc cgacaggctg aaatctttca
     9841 gtccgccaag gatgccgcgg atctggcccc cacgaccagt aatcggctga ggtacgcctt
     9901 ggcgctcgcg acacccggat ttagcggcac ggatgacgtc gcggcccagc gacagttggc
     9961 ggaactcctg gcgaggcctg aaactctact gccgatagaa cgtttgttgg ccctcgtgga
    10021 gctcaaagag gtcgagcaga ggctgatact tcaggctgaa aacaagcgcg tgagggacga
    10081 tgcccaacac gatgattcgc gcgacaagct cgccacggct aaccgtcgct tggccgccga
    10141 gtcggacgaa aatgtgagat tacgcaaggc cttggacgag gctcgcgcca agctcgaggc
    10201 ggtcactcac atcgagcgct cgatcaacga ccgcggaacg agtggatcgc acacaccgtg
    10261 aaagataccg gagagacttc tttgaacgct tattcaattg ccgccacgac tccgctttcc
    10321 ttcgggcttc atcccggtgc ccgggaaacg cagaccccgc agaagcgcaa ggcgcggatt
    10381 ctggtcgtag atgatgaccc cggattgtta cgactgttga cgattcgcct gcgcgccgag
    10441 aactacgaag tcgaagcggt cgagagcgcc gcggcggcgt tggcggcctg cttgcggttt
    10501 cggcctgatc ttgtgatcac cgacttacgc atggatcaaa tggacggcat tgggctgctc
    10561 aaggagttgc agagccgttg gcccggtttg cgcgtgatca ttctgaccgc ccacggcacg
    10621 atcccggatg ccgttcatgc cacccaaagc ggcgccttcg ggtttctgac caaaccggtc
    10681 gacaagcaag acttgctcga tcacgtccag aaggccctca aggtctcggg atttgcgcac
    10741 gtcgatgagg actggcgctc gaacatcatc acccgcaacc agctgatgga agacaagctg
    10801 gcgcaggcca acatggtggc cggtaccgat gcacgggtgc tcctcaccgg cgagagcggc
    10861 acgggcaagg agctgttggc gcgcgccatc cacgaggcca gcccacgccg caacaagccc
    10921 ttcatcgcca tcaactgcag tgcgatggcg gaaaacctct tggaatccga actctttggc
    10981 cacgaaaagg gcgcgttcgc cggggccacg cgttcacaaa agggcctgtt cgaaacggcc
    11041 gacggcggca ccttgctgct cgacgaagtg ggcgacatgc cgatgcggct gcaggtaaaa
    11101 ctgctgcgcg ttctgcaaga aaaccagatc cggccggtgg gggccgcgga gacggtgccg
    11161 atcaacgttc gaatcatttc cgcaacccat cgcgacttgc acgaattgat ggtcggcggg
    11221 caatttcgcg aggatttgta ctaccggctg aacgtcgtgc acatcgagat gccgggtttg
    11281 gggcggcgcc gcgaggacat tcccttgctg gtcgcgcatt tcctggaaca gattgccaag
    11341 gagagcgggc aacgaaaaat ttacgcgccg gaggcggtgg aattgctggc gacggccgaa
    11401 tggccgggca acgtgcgtca gctgtacaac gtcgttcgtc aaaacgtggc gctgtcgcac
    11461 ggcgcaatta tcaccgccga actggttcag caatcgttgg gcggcaaccc gacgcggctg
    11521 ccctctttcg acgaggcgcg cgacgaattc acccgcaatt acctgtcgca gatcctgcag
    11581 atcaccggag gcaatgtcag ccaggcggca cgattggcga agcgcaatcg caccgacttc
    11641 tacaagctgc tgtcgcgcca ccagctgctg cccgacgatt tcaaaaagag ttaggaatag
    11701 aactcggcga tcgtcgagac gacgtgcgcc agctgcgctt cctcgagttc cggatagatc
    11761 ggcaacgcga gggtctcgtt cgccgcccgt tcagactcgg gaaaatcccc ggcccggtag
    11821 tgc
//
LOCUS       DASWZT010000451         7629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_76024, whole genome shotgun sequence.
ACCESSION   DASWZT010000451 DASWZT010000000
VERSION     DASWZT010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7629
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76024"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..264
                     /locus_tag="VGE92_12790"
     CDS             55..264
                     /locus_tag="VGE92_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314751.1"
                     /translation="MRNPPDKWSPMEPDGPDSRPQPAATRRGALLGLIVVVLLVAGGL
                     ALTRILHGMAQLQDCALSGRSNCAQ"
     gene            complement(271..1929)
                     /gene="treF"
                     /locus_tag="VGE92_12795"
     CDS             complement(271..1929)
                     /gene="treF"
                     /locus_tag="VGE92_12795"
                     /inference="COORDINATES: protein motif:HMM:NF009774.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HEY1314752.1"
                     /translation="MTAKNTTPRCVRCASARRNLAWAFSLLLPIAAALPRTASAPEAP
                     PQELFQDLFIAVQSAHLYADGKAFPDAVPKTAPADILRQYHTQRPESPAALKQFVEAH
                     FAVPSEVNAVPSPPDQVSIVAHIDGLWNRLTRTTTTAPPYSSLVAMPQPYVVPGGRFR
                     EMYYWDSYFTMLGLADSGRRDLVDDLTRDFAYLIDTFGHVPNGARTYYLSRSQPPFFF
                     AMVALNSPQDPAAAFAQFLPQLRKEYAFWMEGEAALKPDTAHRRVVMLPDGSVLNRFW
                     DDRDTPRDESYNQDMDLAHLSGRPAPQLFRDVRAGAESGWDFGSRWFADGRSQATIDT
                     TEILPIDLNSLLYGLENAIHLGCARSGDKACDGEFEQRAKSRHAAIDRYLWDAPRGAY
                     LDYRWTQKARSPTLSAATLYPLFVALASQPQASAVAKTTARELLKPGGIVTTTVDTGQ
                     QWDSPNGWAPLQWIAVSGLRDYAQTSLAATIACRWMGNVNELYRQSGKLVEKYNVVTI
                     GRGGGGEYPLQDGFGWTNGVMRRLMVLYPGQADVSTPAQCPAGG"
     gene            2439..4322
                     /locus_tag="VGE92_12800"
     CDS             2439..4322
                     /locus_tag="VGE92_12800"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEY1314753.1"
                     /translation="MKWLTSKRQVKMIYAVAALVTTCGAAMGASDSEQFQRFQDPNQW
                     GAPAGNLNQTRYSSLKDINTGNVKGLSMIWAQSSGTLRGHEGQPLVIEDVGGKPMMFI
                     ESGWPNILQGLDLSDPDHPMQVWNYTKTSGRDESAVPRACCDTVNRGMSYADGKLVFG
                     TLDGYVIALDAATGKEIWVVKHAHPDKGETITPAPIIANDKVIIGFGGDEFAARGRMT
                     AYNLADGKKVWECHSTGTDADVCLTKDTNKKHPEYGKFGKDLGISTYVGDEWKRGGGA
                     AWGWYAYDPALHLVYYGTGNPGLWSPQFRCAEPLTQANCNSGKFDNKWSMSIFARNVD
                     TGAAEWVYQMTPFDQWDYDGINEVVLVDGTIDGKARKLLVHFDRNGFAYVFDRTDGTL
                     VRANKYVTTNWAEKIDMKTGRPVKVPEHSPLKVGENTQACPSAMGGKDQQPVAVDPSD
                     PLNFYVPTNNWCMELEPQQRSHTTQGTVYVFANVYMYPEKPGVTGKFKKFNIDTGKTE
                     WEIPDSYPNWGGALVTGGGLAFYGSLGGDFRAVDRSNGQVLWSKKLGSGIIGNPITYS
                     VKGKQYVSVWSGIGGWIGLPVTAGLDLNDKFGAIGATAMTKAANLSHIPQGGTLYTFR
                     IGD"
     gene            4499..6133
                     /locus_tag="VGE92_12805"
     CDS             4499..6133
                     /locus_tag="VGE92_12805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated putative
                     ABC transporter substrate-binding protein"
                     /protein_id="HEY1314754.1"
                     /translation="MNSKDGARVRQVILAAALLSVGGIALGDDLKSLTVCADPGNMPL
                     SNQKGEGFENKIAQVIGTALGTGVQYYWRPSIERGLMRTTLSEGNCDLWMDMATDTEG
                     AVVLKPLYRSTFVLAYRSDKGINIKNLDDPELKKLRVGVFQVSAIRQALADHKVVSNT
                     VVHYLSHNADIVANNQPSYQIQQVVDGGLDIAAAWGPMAGYYKAVLKAPLIIQPVNTM
                     EDHVAMEFDMALAVPRGRPDIKAAIEQALEQHQTEIHQILTEFGVPLVKCSECYVSGD
                     LPEHGPYNEATPQMRTAYDPADQRARMAELKKSLAQGARPDDEMNNAIVAGDLDRVRY
                     LAAHGAHVDAVDGDGYPALTNATRFGFIDVATFLAEHKADVNGTDRSGWTPLMYAAWD
                     DQPAMIDMLLKHGAKLDATEHEGLTPIAIAVQNAKPKAARALLDAGADVNAPVAKGGY
                     SPLMLAAISGSTEVAAMLIERGAKVNAANAGGVTALMIAAAGNRSAIVGFLLKSGADV
                     NARSEDGRTALSIAQANNSDAVVKILKDAAGGAKSG"
     gene            6148..6678
                     /locus_tag="VGE92_12810"
     CDS             6148..6678
                     /locus_tag="VGE92_12810"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY1314755.1"
                     /translation="MRRSVVLVGMVLTVFAAVSALCGLGRVWADDAKAPVDYTKILPA
                     DLVKNTPKGQLHNPYKDTQADVVAEGGKFLLSYSCSGCHGGGGGGGMCPALTNDIWVY
                     GGDDDTLFRLVTLGSAELQKAGYTRVGRENVVGPMPPFGAIIKSADDLWRILAFVRSK
                     YSGDPAYKFGTPPDQQ"
     gene            6832..>7629
                     /locus_tag="VGE92_12815"
     CDS             6832..>7629
                     /locus_tag="VGE92_12815"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY1314756.1"
                     /translation="MSMSNLATSHASRILHVVQNGLQPAGIEPHVTRSWYRCLREYRI
                     EPSAPRQNTVLNSQSLKELQQRMGELLPVARAEMESLYEQIAGSGFAVILSDTQGVVL
                     TTITDPTLQREFRHAGLSLGALWDERHEGTNGIGTCLAEGGPVTVHREEHFRGYNLSL
                     SCSGAPIVDPHGSIIAVLDASTANSSDSRLIQRHTMALVNMSAHLISRWNFLNEFSQS
                     WILRFHSRPEFVGLLHEALVAMDGAGHILAVNESALVQLGCSTRKALV"
BASE COUNT         1565 a         2283 c         2390 g         1391 t
ORIGIN      
        1 cgcttaagat ccgcggattt gcatgcccgc cgctaaagtc agcagaatgg cgctatgcgc
       61 aatccccccg ataaatggtc gccgatggag ccggatgggc ccgactcgag accgcagccg
      121 gccgcgacgc gccgcggtgc gctgctcggc ctgatcgtcg ttgtgttact ggtggcggga
      181 ggtttggcgc tcacccgcat cctgcacggg atggcgcaac ttcaggattg cgccttgtcc
      241 gggcgctcga attgcgcgca ataaaataac ctacccgccg gccgggcatt gtgccggcgt
      301 cgatacatcc gcttggccgg ggtacagcac catgagccgg cgcatcacgc cattggtcca
      361 gccgaagccg tcctgcaacg ggtactcgcc gccgccgccg cgaccgatgg tgacgacatt
      421 gtatttctcc accagtttcc cgctctgccg atagagctca ttgacgttgc ccatccagcg
      481 gcacgcgatc gtcgcggcga gcgaggtttg ggcgtaatcg cgcagacccg acaccgcgat
      541 ccactgcagc ggcgcccagc cgttcgggga atcccattgt tgccctgtgt cgacggtcgt
      601 cgtgacgatg ccgccgggct tgagcaattc tcgcgccgtc gttttcgcca ccgcgctcgc
      661 ttgcggctgc gaggccaacg ctacgaacaa gggatacagg gtcgctgcgg ataaggtcgg
      721 tgagcgggct ttttgcgtcc accgatagtc gagataggca cctcgaggtg catcccagag
      781 gtagcgatcg atcgccgcgt gccgcgattt cgcccgctgt tcgaactcgc cgtcgcaggc
      841 tttgtcgccg ctgcgtgcgc agcctaagtg gattgcattc tccaagccgt acagcagact
      901 gttcaaatca atgggtaaga tttccgtggt gtcgatcgtc gcttggctgc ggccgtcggc
      961 gaaccagcgc gagccgaagt cccagccgct ttccgcgccg gcgcggacat cgcgaaacaa
     1021 ttgcggcgcg ggacgaccgc tcaagtgcgc aagatccata tcctggttgt acgattcatc
     1081 gcgcggcgtg tcgcggtcgt cccaaaagcg attgagcacc gaaccgtcgg gcaacatcac
     1141 cacgcgccga tgcgccgtat ccggcttgag agccgcttcc ccctccatcc agaaggcata
     1201 ttccttgcgc agttgcggca ggaactgggc aaaggccgcc gcgggatctt gcggcgagtt
     1261 aagagccacc atggcgaaga agaagggcgg ctgcgagcgg cttaaatagt aggtgcgcgc
     1321 gccgttcggc acgtggccaa aggtatcgat caagtaggca aaatcgcgcg tcaggtcgtc
     1381 aaccaagtcg cgccggccgc tgtccgcgag accgagcatc gtgaagtaag agtcccaata
     1441 gtacatttca cgaaagcggc cgcccggcac cacatagggt tgcggcatcg ccacgagcga
     1501 tgaatagggc ggcgccgtgg tggtcgtgcg cgtcagccga ttccagagtc catcgatgtg
     1561 cgcaacgatc gagacctgat ccggcggcga aggcacggcg ttgacttcgg acggcacggc
     1621 gaaatgcgcc tcgacgaact gtttgagcgc agccggcgac tcgggccgtt gcgtatggta
     1681 ctggcgcaat atatcggctg gtgccgtctt cgggaccgca tcgggaaaag ccttgccatc
     1741 cgcgtagaga tgcgccgatt gcacggcgat gaacagatcc tgaaacagct cctgcggcgg
     1801 ggcctcgggc gcgcttgcgg ttcgcggcag cgcggctgcg atcggcaaaa gcagcgagaa
     1861 tgcccaggca agattgcggc gagcggacgc acagcgcacg cagcgcggcg ttgtattttt
     1921 tgctgtcatg gcgcgtgcga atttatcaga acgcatgtgt cggcgcggca acagacggct
     1981 cgcggcaggc aaaaaaataa attgcggcgc tgcgcctcac taacggcgca atccccatgc
     2041 tccactgtca cgcgcatgac acagttcgtc cgcgacctcg ccgtgagcgc ttcccgggga
     2101 attcgtaaat agctgatttg gaaaggtttg ttattacgcg tgattttctt ttgtcatttg
     2161 ctgaagatgg cataggcatt gctattccaa catggcggcc caaggcggtg gtgcctctcg
     2221 ctgacttgcc atctcattgg gatcagacta gccgcggcac gatcgcggct ggacaactat
     2281 aagtaattgg taagcattga gaaggaggga gttttggtag tcacgattaa caacgatcaa
     2341 cgcaaatacc gcacggcact caggattacg cactgtgtgc acgccgcctg caacagcagc
     2401 ggcaatcggc tcggacccca cgcagcagga gaatttcgat gaaatggctt acgtcaaaac
     2461 gtcaggtcaa gatgatttac gccgtcgcag cgctagtaac gacctgtggt gccgcgatgg
     2521 gcgcttcgga ttccgaacag ttccagcgct tccaggaccc caatcagtgg ggtgcgccgg
     2581 cgggcaattt gaatcaaacg cgctatagct cgctgaaaga catcaacacc ggcaacgtca
     2641 agggcctgag catgatctgg gcccaatcga gcggcacctt gcgcggtcac gaaggccagc
     2701 ccttggtgat cgaagatgtg ggcggcaagc cgatgatgtt cattgaaagc ggctggccca
     2761 acattctgca gggactcgat ctctcagatc ccgaccatcc gatgcaggtg tggaattaca
     2821 ccaagaccag cggacgcgat gaatcggcag tgcctcgcgc ctgctgcgac accgtgaacc
     2881 gcggtatgtc ctatgccgac ggcaaactgg tgttcggcac cttggacggc tacgtcatcg
     2941 ccctcgatgc cgcgaccggc aaggaaatct gggtggtcaa gcatgcccat ccggataagg
     3001 gcgagaccat cacgccggcg ccgatcatcg cgaatgacaa agtcatcatc ggcttcggcg
     3061 gcgatgagtt tgcagcgcgc ggccgcatga cggcctacaa cctcgcggat ggcaagaagg
     3121 tgtgggagtg tcattccacc ggcaccgatg ccgatgtgtg cctgaccaag gacaccaaca
     3181 agaagcatcc ggaatacggc aaattcggca aggacttggg tatcagcacc tatgtgggtg
     3241 acgagtggaa gcgtggcggc ggcgctgcgt ggggctggta tgcctatgac ccggcgctgc
     3301 acctcgtgta ctacggcacg ggcaacccgg gtttgtggtc gccgcagttc cgttgcgcgg
     3361 aaccgctgac gcaagccaat tgcaacagcg gcaagttcga caacaagtgg tcgatgagca
     3421 tattcgcccg caatgtcgac accggggctg ccgaatgggt ctatcagatg acgccgttcg
     3481 accagtggga ctatgacggc atcaacgaag tggtgttggt cgacgggacc atcgatggca
     3541 aggcgcgcaa gttgctggtg cacttcgacc gcaatggttt cgcgtatgtg ttcgaccgta
     3601 ccgacggcac gctggtccgc gccaacaagt acgtcaccac gaactgggcc gaaaaaatcg
     3661 acatgaagac cggtcgaccg gtcaaagtgc cggaacattc gccgctcaag gtaggtgaga
     3721 acacgcaggc ctgcccgtcc gcgatgggcg gcaaggacca gcagccggtg gcggtggatc
     3781 cgagcgatcc gctcaatttc tatgtgccga ccaacaactg gtgcatggaa ctcgaaccgc
     3841 aacagcgttc gcacaccacg cagggcacgg tgtacgtgtt cgccaacgtc tatatgtatc
     3901 ctgaaaagcc gggtgtaacc ggcaagttca agaagttcaa tatcgacacc ggcaagaccg
     3961 agtgggaaat cccggattcg tatccgaact ggggtggcgc actggtaacc ggcggcggct
     4021 tggccttcta cggcagcttg ggcggcgact tccgcgccgt cgatcgctcg aacgggcagg
     4081 tcctgtggtc gaagaaactc ggctccggca tcatcggcaa cccgatcacg tactccgtga
     4141 agggcaagca gtatgtgtcg gtctggtcgg gaatcggcgg ctggatcgga ctcccggtca
     4201 cggcgggatt ggacctgaac gataagttcg gcgcgatcgg tgccaccgcg atgaccaagg
     4261 cggccaactt aagtcatatt ccacagggcg gtacgttgta cacgttccgg ataggcgatt
     4321 aaaggtcgat attgattcat tcgtgttgga gagatgtgcc cggtgacgca agtcaccggg
     4381 ctttttttcg gggctcgcgc ggcgcaatat ggcagccgcg cactgcaacc ttcgcggttc
     4441 gcgcaagtcc aatggtgagt gctggcgggg cgcgaaccgc cctttcaagg agattgcatt
     4501 gaatagcaaa gacggagcgc gcgtccgaca agtgatcctt gccgccgccc ttttgagcgt
     4561 gggtggtatt gcgctcggcg atgatctgaa gtcgctcacc gtgtgtgcgg atcccggcaa
     4621 catgccgctg tcgaatcaga aaggcgaggg attcgagaac aagatcgccc aggtgatcgg
     4681 cactgcgctc ggcaccggcg ttcaatacta ttggcgccca tcgatcgaac gcggcttgat
     4741 gcgcaccacc ttgagcgaag gcaactgcga tttgtggatg gacatggcga ccgacaccga
     4801 aggcgccgtg gtgctcaagc ccctctatcg gtccaccttc gtgctggcgt accgcagcga
     4861 caaaggcatc aacatcaaga acctggacga tcccgaactc aagaaattgc gggtcggtgt
     4921 gttccaggtg tcggcgatcc gccaggcgct cgccgaccat aaggtggttt cgaacaccgt
     4981 cgtccattac ctgagtcaca acgccgacat cgtcgcgaac aaccaaccct cgtatcagat
     5041 tcaacaggtc gtcgacggcg gcctcgatat cgctgcggcg tggggaccga tggcgggtta
     5101 ctacaaggcg gtcctcaagg cgccgttgat cattcaaccg gtcaatacca tggaagacca
     5161 tgtcgccatg gaattcgaca tggcgctcgc agtgccgcgc ggccggccgg acatcaaggc
     5221 cgccatcgag caggctctcg agcagcacca gacggagatt caccaaatcc tgaccgagtt
     5281 cggcgtgccg ctggtgaagt gcagcgaatg ttatgtctcc ggggatttac cggaacacgg
     5341 cccctacaac gaggccacgc cgcagatgcg aaccgcatac gatccggccg atcaacgcgc
     5401 gcgcatggca gagctgaaaa agtcgctcgc gcaaggcgcg agacccgatg acgaaatgaa
     5461 caacgccatc gtggccggtg acctcgatcg cgtccgctat ctcgcggcac acggagcgca
     5521 cgtcgacgcg gtggacggcg acggctatcc ggcgctcacc aatgccaccc gctttggatt
     5581 catcgatgtg gcgacttttc tggcggagca taaggccgat gtcaacggca ccgatcgaag
     5641 cggctggacg cctctcatgt acgccgcgtg ggacgatcag cccgcgatga tcgatatgct
     5701 gctgaagcac ggcgcgaaac tcgatgcgac cgagcacgag ggtctcacgc cgatcgccat
     5761 cgccgtgcaa aacgccaagc cgaaagcggc ccgcgcgctg ctcgacgccg gcgccgatgt
     5821 caacgcgccg gtggcgaagg gcggatatag cccgttgatg ctggccgcga tctccggatc
     5881 gaccgaggtt gccgcgatgt tgatcgaacg cggcgccaag gtgaatgccg ccaatgccgg
     5941 cggagtcaca gcgctgatga tcgccgccgc cggcaatcgg tccgcgatcg tgggctttct
     6001 gctcaagtcg ggcgccgacg tcaacgcgcg cagcgaggac gggcgcaccg cgctcagcat
     6061 tgcccaggcg aataacagcg acgcggtcgt caagattctc aaagacgcag ccggcggcgc
     6121 caaatcaggt taacaaagga gatcggcgtg agaagaagcg tggtgttggt cggtatggtg
     6181 ctgacggtat tcgcggcagt gagcgcgctg tgcggcctcg gtcgagtgtg ggccgacgat
     6241 gcgaaagcgc ccgtggacta taccaagatc ttgccggcgg acctggtcaa gaataccccg
     6301 aaaggtcagc tgcacaatcc ctacaaagac acgcaggccg atgtggtggc ggaagggggt
     6361 aagttcctcc tgagctattc ctgcagcggc tgccacggcg gcggcggcgg cggcggcatg
     6421 tgtcccgccc tcaccaacga catctgggtt tacggcggcg atgacgacac gctgtttcgg
     6481 ctggtcacct tggggagcgc tgagctgcaa aaggccggct acacccgtgt tggccgggag
     6541 aacgtcgtcg gccccatgcc tccctttggc gcgatcatca agagcgccga cgatctgtgg
     6601 cgcatcctcg ccttcgtgcg ctctaaatac agcggcgacc ccgcctataa atttggaact
     6661 ccgcccgacc agcagtaaaa tgctgcagtg cagattgcgc tgaacgcgca aacaggccac
     6721 ccttcgtccc ggctggctgg accttcgcgg gtgcgcggtt ctactgttga cttttcacaa
     6781 caagcgacgg actcgcttgg gaatgagacg agcggtaagt aggggatggg gatgtctatg
     6841 tcgaatctcg ccacgagtca cgcgtcgcgc atcctgcatg tggttcaaaa cggactgcaa
     6901 ccggccggaa tcgaacccca cgtcacgcgc tcctggtacc gatgcttgcg cgaataccgc
     6961 atcgaaccat cggcccctcg acaaaatacc gtgctcaatt cccaatcgct gaaggagctg
     7021 cagcagcgga tgggggaatt gttgccggtc gcgcgcgctg agatggagag cctctacgag
     7081 caaatcgcgg gatccggatt cgccgtcatt ctgtcggaca cccaaggggt ggtgctgacg
     7141 accatcaccg accccaccct gcaacgcgaa tttcgccacg ccggcctgtc gctcggtgca
     7201 ctgtgggacg aacgccatga aggcaccaac ggcatcggca cctgcctcgc ggagggcggt
     7261 ccggtgaccg tgcatcgcga ggagcatttc cgcggctaca acctgtcgtt gtcctgctcc
     7321 ggcgcaccaa ttgtcgatcc gcacggctcg atcatcgccg tactcgatgc gtcgaccgca
     7381 aattcgagcg acagccggtt gattcagcgg cacacgatgg cgttggtcaa tatgtcggcg
     7441 cacctcattt cgcgctggaa ttttctgaac gagttcagcc agtcctggat cctgcgcttc
     7501 cacagccgac cggagttcgt cggactgttg cacgaggcgc tggtggccat ggatggcgcc
     7561 ggccacatcc tcgccgtcaa tgaaagcgcc ctggtgcagc tcggctgctc gacgcgcaaa
     7621 gcattggtc
//
LOCUS       DASWZT010000452         1895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_90473, whole genome shotgun sequence.
ACCESSION   DASWZT010000452 DASWZT010000000
VERSION     DASWZT010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90473"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1895)
                     /locus_tag="VGE92_12820"
     CDS             complement(<1..>1895)
                     /locus_tag="VGE92_12820"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HEY1314757.1"
                     /translation="MLQADIASISPEELRTYKLGSILAGGNAAPGNNVHAAPQAWLDM
                     TKAYHRASLRATSAKHPPIPILFGIDAVHGHAKVIGATIFPHNVALGAAHDPDLVRRI
                     GRATAEEVASTGIDWTFAPTVAVARDVRWGRSYESYSESPQLVAEYAAAMVSGLQGER
                     GTPEFMAPGHTLSSVKHFLGDGGTSGGRDQGDTMVSEAQLSAVHGAGYPAAINAGALI
                     VMASYNSWNGVKMHANHYLLTDILKGRLGFQGFVVGDWNAHEQIPGCTKYRCAAAILA
                     GVDMLMAPDSWRELYKNTLAQVHSGEIPQSRVDDAVRRILRVKTLAGLFKRAAPTQRG
                     DSGDFLRLGSAAHRALAREAVRKSLVLLKNDHHTLPLDPRANILVAGEAADSIAAQSG
                     GWTIDWQGDHNSNADFPGATSIFGGIRAAAAAAGGSAVLSTDGKFSDKPDVAIVVFGE
                     APYAEFEGDRENLEFSPNDKHDLALLRRLHAAGVPTVAVFLSGRPLWVNPELNASDAF
                     VAAWLPGSEGEGVADVLIRPMAGAAADFTGRLAFSWPDTAMPVNFDDAGKVSGALFSQ
                     GWGLDYHTTLQSQRLSEDSRIAPRWVSPHGQLVHAGHVTAPWSIFVADDTAQVRLTTA
                     RQESPQGA"
BASE COUNT          342 a          635 c          586 g          332 t
ORIGIN      
        1 gctccctgcg gactctcctg ccgcgccgtg gtcagacgca cctgcgcagt gtcatctgcg
       61 acgaagatgg accacggcgc ggtgacatga ccggcatgaa cgagttgtcc gtgggggctg
      121 acccaacgcg gcgctatgcg cgaatcctca gacagccgct gcgattgcag ggtggtgtga
      181 tagtcgaggc cccatccttg ggagaacagc gcgccggata ccttacccgc gtcatcgaag
      241 ttcacgggca tcgcagtgtc cggccacgag aacgccaggc ggccggtgaa gtccgcagcg
      301 gcgcccgcca tcgggcgaat caagacatcg gcgacgcctt cgccctcgct tccaggcaac
      361 cacgcggcga cgaaagcatc cgatgcattg agctccgggt tgacccacag aggtcgcccg
      421 gacaagaaca cggcgacggt gggcacgccc gcggcatgca agcgccgcaa cagcgccaga
      481 tcgtgcttgt cgttcggcga gaattccaga ttctcgcgat cgccctcgaa ttcggcgtaa
      541 ggggcctcgc cgaataccac gattgcgaca tccggcttgt ccgagaactt cccgtccgtg
      601 ctcagcaccg ccgaaccgcc ggccgccgcc gccgccgccc gtatcccgcc gaagatggac
      661 gtggccccgg gaaaatcggc attgctgttg tgatcgccct gccagtcgat agtccagccc
      721 cccgactgcg cggcgatgga atccgccgcc tcaccggcaa ccaagatgtt ggcgcgcgga
      781 tccaatggca gcgtgtggtg atcattcttc agcagcacga gggatttgcg caccgcttca
      841 cgcgccagcg cgcggtgcgc tgcgctgccg agccgaagga aatcgccgct atcgccgcgt
      901 tgcgtcggcg ccgcgcgctt gaacaggcca gccagggtct tgacccgcaa aattcgccgt
      961 accgcatcgt cgacacggct ttgcggaatc tcgcccgaat gcacctgcgc caaggtgttc
     1021 ttgtagagtt cccgccagct gtccggcgcc atcagcatat cgacgccggc gagaattgcg
     1081 gccgcgcacc ggtacttggt gcagcccgga atctgttcat gagcgttcca atcgccgact
     1141 acgaatccct gaaagccgag ccgccccttc aatatgtcgg tcaacaggta gtggttggcg
     1201 tgcatcttca cgccgttcca actgttgtac gatgccatca cgatcaaggc gcccgcgttg
     1261 attgccgccg ggtacccggc gccatgcacg gcgctcaatt gtgcttcgga aaccattgtg
     1321 tcgccctgat ctcgcccgcc cgacgtgccg ccgtcgccga ggaaatgctt gaccgacgag
     1381 agcgtatggc ccggtgccat gaactcagga gtgccgcgtt caccttgaag accgctcacc
     1441 atcgcggccg cgtactcggc caccaactgc ggcgattcgg aatagctctc gtaagatcgg
     1501 ccccacctca catcgcgcgc aaccgccacg gtgggtgcga aggtccagtc gatgcccgtg
     1561 ctcgcaactt cctcggcggt ggcccgtccg atccggcgga cgaggtcagg atcgtgcgca
     1621 gcaccgagcg ctacattgtg cggaaagatc gtggcgccga tgactttcgc atgtccgtgc
     1681 acggcatcga tgccgaacag aatcggaatc gggggatgct tggcgctggt tgcgcgcagc
     1741 gaggcacgat gataagcctt ggtcatatcc agccacgcct gcggcgcggc gtgcacgtta
     1801 ttcccggggg ccgcatttcc gcctgccaga atcgacccca gtttgtaggt gcgcagttcc
     1861 tcgggcgata tcgacgcgat gtcggcctgg agcat
//
LOCUS       DASWZT010000453         9661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_61575, whole genome shotgun sequence.
ACCESSION   DASWZT010000453 DASWZT010000000
VERSION     DASWZT010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9661
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61575"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VGE92_12825"
     CDS             complement(<1..310)
                     /locus_tag="VGE92_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug ABC transporter ATP-binding protein"
                     /protein_id="HEY1314758.1"
                     /translation="MFRYFENLVSPYPNAPPPPLPQGFFAFLWACARGVRRYIAAMTL
                     FTAVIGVFEALLFGFLGRVVDWLAKIPPARLWAEQKGPLLLLAGILAGSTALIGLQSL
                     Y"
     gene            442..606
                     /locus_tag="VGE92_12830"
     CDS             442..606
                     /locus_tag="VGE92_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314759.1"
                     /translation="MQEQFFAPQRPNPTQFGSSDGQQARDAWKAQLDQWLHEYRDRYV
                     LRQPHEKHAP"
     gene            660..1280
                     /locus_tag="VGE92_12835"
     CDS             660..1280
                     /locus_tag="VGE92_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314760.1"
                     /translation="MHKRTTAGGMAGAFMVALVIAFLARHGAAPGEKLALALKATARW
                     SFLLFWLGYAGSSLANVFGPKFRALAHHGRDFGLAFAAAHLVHLALVAWLLHNLTRPF
                     PRPALTLLGIGVFCICLLSILSIKPLAATIDRRLLRTLRFVAVEYIALVFLIDFAKNP
                     LQGGVATLIAYLPFQILAISGLLLRIASAVKRESVHYALQPRWRNW"
     gene            complement(1582..2313)
                     /locus_tag="VGE92_12840"
     CDS             complement(1582..2313)
                     /locus_tag="VGE92_12840"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY1314761.1"
                     /translation="MRCAPRAAFPASRRSPTSAMEPRNRRPRVRPAGIIVTRVRRPAI
                     RTAQVTVLFADLRGYTGLAERLSAARVVPLLDEFLRTLAAAAERFGGTIFHMAGDGLM
                     AGFAVDDERGNGAREALAAGRAMLEEFAPIAARWRKELKIEAGIGVGLHLGDVAVGAL
                     GPSGLQAITLIGDTVNVAARLCSRARAGEVLFSCTVAAALEIAGATPTAAGEVPFLQL
                     PQFELRGRRGPIDIWCVPAASRLAL"
     gene            complement(2261..4312)
                     /locus_tag="VGE92_12845"
     CDS             complement(2261..4312)
                     /locus_tag="VGE92_12845"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY1314762.1"
                     /translation="MRTVIVFAGLLLTQLAAAETTATRIAVAELAKPPAAAEHFSVVS
                     TAGKHGDSSRWTTADGLRMGRESFVLRGQVFEQDSAAQVGADGMLSRVTIRGFTPNGD
                     AAESFSIAHGRATWKSPVDGGSAAYSAPALYVAFGGPVDLTADFAERLIAAPDKSLAL
                     LPAGRAHAETLASASVGTGAAKKQLTAYAMTGVSNTPIPVWMDESGRFFASVGSLSWI
                     RSGFESSAALLQKTQDEALAKRSPALVKSLLKTPPGPVAFTHVRAFLAGDHFAEDQTV
                     IFAKGVITQARAASEVTVPAGAQVIDGRGKTLVPGLWDSHMHFQDDASGPALLSLGIT
                     SARDPGNNNALTLARAQRRAQGLLLSPRIYPSVLIDGKGPNTAQSGSVATSQEEALAI
                     VRKAKAQGFVAIKIYGSFNPAWVKATAAEAHRLGLHVHGHLPAGMRTSQAIDDGYDEI
                     THIYFVMMQAMPDEVVNQSNGMARFLGPGRYAKDVKLDAEPMKSLIGKMAQRKITVDP
                     TLVVAESLYVPENGDLSPAYAPFVGTLPPAVERGFRQGGFAMPPGVTRADFRASFNKL
                     SQLVGALHEAGVPIVAGTDGSGMELVRELELYVAAGFTPAQALASATIQTAHLVGADA
                     HTGSITVGKAADLVLVDGNPSKHVGDLRHTLIVVMDGKMMDADALRAAGGFSGKPALA
                     D"
     gene            4368..5348
                     /locus_tag="VGE92_12850"
     CDS             4368..5348
                     /locus_tag="VGE92_12850"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY1314763.1"
                     /translation="MLSLPMFARACALSLALLCGAAAAASLIAPGTYLIEGEATPNRQ
                     PDGNSIVIAAARGLIVFDTGRHREHAQQVLDFARAQAQPIVAIINSHWHLDHVGGNPL
                     LRAAFPDIVVYASSAIDAARTGFLADYRAQLLTQIAAAKQPESIAAMRAEIALIDQGA
                     ALGPTVVIARSAALNIAGRRLQLHLESHAVSAGDVWVFDPETRVLLAGDLVTLPAPFF
                     ESACPARWRASLEHLGKVHFDWLVPGHGKPMRRKEFSAYTTAFANLLACAGGGATRSA
                     CIDGWINDASALVESKDVSYARTLIGYYVDNYLHNPSERVRNFCSDRPAH"
     gene            complement(5345..5713)
                     /locus_tag="VGE92_12855"
     CDS             complement(5345..5713)
                     /locus_tag="VGE92_12855"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY1314764.1"
                     /translation="MTIRSFEIVSVPVSDQEKSKQFYRDVLGFELLREEPMGPGQKWI
                     QLAPKGCSTTIALVMWFDGMRPGGLQGVMLNCSDIERDHADLTQRGLALTEIKQEPWG
                     RYSMFKDPDGNGWILRQPPG"
     gene            complement(5723..7015)
                     /locus_tag="VGE92_12860"
     CDS             complement(5723..7015)
                     /locus_tag="VGE92_12860"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEY1314765.1"
                     /translation="MKAAWLLLIALGTECYAAPPDTDPAQGPATGPIAPPPAAPATGL
                     SRWFNPGTAPFIPVPEIAVDPDSGTTLGLLPTWIKTDDNHEIRRIIAPDIIYNPYFGV
                     GVHGRIYGYTSEDEQWSVVSGIQEHVEREFEAEYQVGRLRQDRWSFNTRVISDRDGTP
                     RFYGIGNESPAIAETNYTNQQETLQQQVGLNFSHAWQLLYTARFEDVDVLPGTLEKVA
                     TIGSRFSRSLGIGTNKQILNRVSLVYDTRDNLVVPSRGMQLIAYGGLASRKGLLDDSM
                     YSEVGGDGRAFWPLAKDTILAGHMSLRYMPTSHDVPFWALSNIGGGESVVGGEQPLRG
                     FGAGRFYDRDSFSSTVELRRKVITFDATSTLVDVEVTPFIDVGRVFARTSTLPFEQLH
                     HVYGVGFRGIARPFVVGYVDIGYGSEGVAAFTGINYPF"
     gene            complement(7222..8280)
                     /locus_tag="VGE92_12865"
     CDS             complement(7222..8280)
                     /locus_tag="VGE92_12865"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY1314766.1"
                     /translation="MNSPNAFDLRRVGAHPDYWYPLAWSHELKVGKTLGRRFAALPIV
                     LYRGKSGQVFALEDRCAHRQVPLHLGVVSGDALKCHYHGWSYNGTGKCVNVPYLGTER
                     LPTGVKSYPAREVDGLIFVFPGDAAFADARLPANLGSSQRKDYKTRRLNREVACHYTF
                     MHENLFDMNHQFMHRKQMGSISARCLGRAHGDNWAQVEYSFSRTEGKSSVGEHVIVDL
                     MRKRGEAKKDFCDHMRIRTDYPTQSLKVWVGRDIQQTDDPVLDVWLGYTPLDAEQRTN
                     RTYGYLSVKKPKLPGIIHAVWPFVTWFTENIFREDKEIVEYEQRAHDAQGGDWNNEVF
                     PPIRDLRGVLARCGKPID"
     gene            8625..>9661
                     /locus_tag="VGE92_12870"
     CDS             8625..>9661
                     /locus_tag="VGE92_12870"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY1314767.1"
                     /translation="MHTAADAQRAANRWLVEINFLRATRMLVTASAAAGVIFATPAGA
                     QQAPSSSSNQLQEIVVTGSLIKRTDTETPSPVQVITAEDLSNSGFNNVSDVLRNLAAN
                     GQGTLNQGFGQAFASGGSGIALRGLTVGDTLTLIDGERMVAYPLSDDGERSFVDISAI
                     PFNAIDTVEVLKDGASALYGADAIAGVVNVKLKQTYVGSEITAEGGETQRHDGQTYHA
                     SAILGWGDLANDGYNIYAALDWHRQETILGSNRSGAWTNLDWSGLGGQNTNGGAIGAT
                     GLVYPDSSTGYLLNPTTPNAAGVYPNGLPGEVFLPGCTATLQAADKCTFTYPGEIQPP
                     TEQTNFLAKFT"
BASE COUNT         1814 a         3126 c         2954 g         1767 t
ORIGIN      
        1 tgtacaggct ctgcaagccg atgagtgcgg tgctgccggc gaggatcccg gccagcaata
       61 gcaagggacc cttctgctcg gcccacagtc gcgcaggcgg aatcttggcc agccaatcga
      121 cgactcgacc gagaaagccg aacagcaacg cctcgaatac gccgatgacg gcagtgaaca
      181 aagtcattgc cgcgatgtat cgccgcacgc cgcgggcgca cgcccagagg aaggcaaaaa
      241 agccctgcgg cagcgggggc ggcggcgcat tcggatacgg cgagaccaga ttttcaaagt
      301 agcgaaacaa gcgcgaagta tagccttgtc gcttaacggt cgctgcactg caatgcgttt
      361 gtcatgccgc ggcgctagca ttcggcacgg tcaggtgtaa aatccgacgc ccccatggcg
      421 agcaactttg tggagactga catgcaagag cagttcttcg cgccacagcg ccccaacccg
      481 acgcagtttg gaagttccga cgggcagcaa gcacgcgacg cctggaaagc ccaactcgac
      541 cagtggctgc acgaatatcg agaccgctac gtgctgcggc aaccgcacga aaagcacgca
      601 ccctagcctg aagtttccgc gcgcgttacc atcaccccca caggaagggg tgttctgcaa
      661 tgcacaagcg aacaacggcc ggggggatgg ccggagcgtt catggtggcg ctggtcatcg
      721 cgttccttgc gcgccacggc gcagcgccgg gcgaaaaact ggcgcttgcg ctcaaggcca
      781 ccgctcgctg gtccttcctg ttattttggt tgggatatgc cggcagctcg ttggcaaacg
      841 tcttcggccc gaagttccgc gccttggcgc accacggccg cgattttggt ctcgcgttcg
      901 ccgcggcgca tctcgtacat ttggctctcg ttgcctggct tctgcacaac ctcacgaggc
      961 cgtttccgcg gcctgcattg acactcttgg ggatcggcgt tttttgcatc tgcttgctgt
     1021 cgattttgtc gatcaagccg ctcgccgcga caatcgatcg caggctattg cgaaccttgc
     1081 gcttcgttgc ggtcgagtac atcgcactgg tcttcttgat cgacttcgcc aagaatccgt
     1141 tgcaaggcgg agtggctact ttgatcgcct acttgccgtt tcagatcttg gcaatcagcg
     1201 ggctgctgct gcggatcgca tccgccgtga agcgggaatc ggtacactat gcacttcagc
     1261 ctcggtggcg aaattggtag acgcaacgga cttgaagaaa ttgagcggca ggtaggaaac
     1321 cgcctgctgg atcccatcaa agtcggtgaa cccttggact tccgaggcaa cgccgagcca
     1381 atcctgccga cgaccgcgtg gacaggcagg tgtagagagc ggacggtggg ccctaacgca
     1441 gcaacacgct gttatggtgg tggcgcgctc cagaccccaa acgcgcgcaa gcgcggcgac
     1501 gtaaggcgta gcgggtacga aaatccgttg gccgcaaggt catgccggtt cgaccccggc
     1561 ccgaggcacc attcgagcgc gctacagcgc gaggcgcgag gccgccggta cgcaccagat
     1621 gtcgatcggt ccgcgccggc cgcgtaactc gaactgcggc agctgcagaa atggtacctc
     1681 gcctgcggcg gtgggcgttg ctccggcgat ctcgagcgcg gcggccaccg tgcacgagaa
     1741 caatacttca ccggctcgag cccggctgca aagtcgcgcc gcgacattga ctgtatcgcc
     1801 gatcagggtg atcgcctgga gcccggacgg cccgagcgct ccgacggcaa cgtccccaag
     1861 atgcagaccg acaccgattc ccgcttcgat cttgagctcc ttgcgccagc gggcggcaat
     1921 cggtgcgaat tcctcgagca tggcgcgtcc ggcggccagc gcctcgcggg cgccgttgcc
     1981 gcgctcatcg tctacggcga aacctgccat caatccgtca ccggccatgt gaaatatggt
     2041 tccgccgaaa cgctccgccg cggcggcgag tgtgcgcagg aattcatcca gcagcggcac
     2101 cacgcgggcc gcggacaggc gttcggccag ccccgtatag ccgcgcaagt cagcaaacag
     2161 caccgtgacc tgtgcggtgc gaatcgcggg gcgccgtacc cgtgtgacta tgattcccgc
     2221 agggcgtact cgcgggcgtc gatttcgcgg ctccatggcg ctagtcggcg agcgccggct
     2281 tgccggaaaa gccgcccgcg gcgcgcaacg catcggcgtc catcatcttg ccgtccatca
     2341 ccacgatgag cgtgtggcgc aggtcgccga cgtgtttcga cggattaccg tccaccagca
     2401 ccaaatcggc cgctttgccg acggtgatgg agcccgtgtg cgcatccgct ccgaccaggt
     2461 gcgcggtttg gatggtcgcg ctggcaagcg cctgcgcggg tgtgaagccc gccgcgacgt
     2521 acagttcgag ctcgcggacc agttccatcc ccgagccgtc ggtgccggca acgatcggaa
     2581 ctcccgcttc atgcaatgcg ccgacgagtt ggctcaattt attgaagctc gccctgaagt
     2641 ccgcgcgggt cacgcccggc ggcatcgcga agccgccctg gcggaaaccg cgttcgacag
     2701 ccggcggcag cgtgccgaca aagggtgcgt aagccggcga tagatcgccg ttttcgggaa
     2761 catacaggct ttcggcgacg accaaggtgg ggtccacggt aattttccgc tgcgccattt
     2821 tgccaatcag ggatttcatc ggctccgcgt cgagcttgac gtccttggcg tagcggcctg
     2881 gccccagaaa tcgggccatg ccgttcgact ggttcaccac ctcatcgggc atcgcttgca
     2941 tcatcacgaa ataaatatgc gtgatctcat catagccatc gtcgatggcc tgcgaagttc
     3001 gcattcccgc cggcagatgt ccgtgtacat gcaatcccag acggtgcgcc tcggcggccg
     3061 tcgccttgac ccacgccgga ttgaagctgc cgtatatctt gatcgcaacg aaaccctgcg
     3121 ccttggcttt gcgcacgatt gcgagcgcct cttcctgcga ggtcgcaacg ctccccgatt
     3181 gcgccgtatt cgggcccttg ccatcgatca gcaccgacgg atagatccgt gggctgagca
     3241 acaagccttg cgcgcggcgc tgagcgcgcg ccagggtgag cgcattgttg tttcccggat
     3301 cgcgcgccga ggtgatgcca agcgatagca gtgcaggccc gctggcatcg tcctggaaat
     3361 gcatatgcga atcccacagg cccggcacca aggttttacc gcgcccgtcg atcacctgtg
     3421 caccggccgg cacggtcact tcgctcgcgg cccgagcttg cgtaatcacg cccttggcga
     3481 aaatgaccgt ctgatcctcg gcaaagtgat ctcccgcgag gaaggcgcga acgtgagtaa
     3541 acgccaccgg cccgggcggg gtcttcagca gggatttcac cagcgccggc gagcgcttcg
     3601 cgagcgcctc gtcctgcgtc ttctgcagca gcgctgctga ggattcgaag ccgctgcgaa
     3661 tccacgacaa ggaaccgacc gaggcgaaga acctgcccga ctcatccatc caaaccggaa
     3721 tgggtgtgtt gctgacgccc gtcatcgcat aggcggtcag ctgtttcttg gcggctcccg
     3781 tgccgacact ggcgctggcg agggtttcgg catgcgcacg gcctgccggt agcaaggcaa
     3841 gcgacttatc gggcgcggca atcaagcgct cggcaaaatc agccgtcagg tcgaccggac
     3901 cgccgaacgc cacatacaag gcaggcgccg aataggccgc cgagccgccg tcgaccggac
     3961 tcttccaggt cgcccggccg tgggcgatgg agaaactctc cgccgcatcg ccattcggtg
     4021 taaagccgcg aatcgtgact cggctcagca ttccatccgc accgacctgc gctgcgctgt
     4081 cctgttcgaa cacctgcccg cgcagcacga aactctcgcg gcccatgcgc agtccgtcgg
     4141 ccgtggtcca gcggctcgag tcgccgtgct tgcccgccgt cgataccacg ctgaagtgct
     4201 cggccgccgc cggcggcttg gctagctccg ccaccgcgat ccgcgtcgcc gtggtttccg
     4261 ccgcggccag ctgcgtgagc agcaaacccg cgaacacaat gactgtgcgc ataagtcccc
     4321 ttgatcttct ggcgcaagtc tacacgggtt agaatcgtgg cgaagctatg ctatccctgc
     4381 ccatgttcgc tcgagcctgc gcgctgtcgc tcgccttgct ctgcggcgcg gccgccgctg
     4441 cctcgctgat cgcaccgggc acctatctta tcgagggcga agcgacgccg aaccgccagc
     4501 ccgacggcaa cagcatcgtc attgccgcag cgcgcggctt gatcgtgttc gacaccggcc
     4561 gccatcggga acacgcgcag caggtcctcg atttcgcccg cgcacaggca cagcccatcg
     4621 tcgccatcat caattcacac tggcacttgg atcatgtcgg cggcaatccg ctgctgcgcg
     4681 ccgcgttccc cgatatcgtc gtttacgcca gctccgcgat cgatgcggcg cgcacgggat
     4741 tcctcgccga ttaccgcgcg caattgctga cgcagattgc tgctgccaag cagccggaat
     4801 cgattgccgc catgcgcgcc gaaatcgcct tgatcgatca aggcgcggca ctcggcccga
     4861 ccgtcgtcat tgcacgcagc gccgcgttga acatcgcggg gcgccgcttg cagctgcact
     4921 tggaatcaca cgccgtcagc gcaggcgatg tgtgggtatt cgaccctgaa acgcgggtgt
     4981 tgcttgccgg tgatctggtc accctgcccg ccccgttttt cgaaagcgcc tgtccggcgc
     5041 gctggcgcgc ttcgctcgag cacctcggca aggtgcattt cgactggctc gtgcccgggc
     5101 atggcaaacc catgcggcgc aaggaattca gcgcctacac cacggcgttt gccaatctgc
     5161 tcgcctgcgc cggcggcggc gcgacgcgca gcgcgtgcat cgacggctgg atcaacgatg
     5221 cgtctgcgct cgtcgaatcg aaggatgtga gttacgcgcg aacgctgatc ggctactacg
     5281 tggacaatta cctgcacaat cccagcgagc gcgtgcgtaa tttctgcagc gacagacccg
     5341 cgcactagcc cggcggctgg cgcaatatcc agccgttgcc gtccgggtcc ttgaacatcg
     5401 aatagcggcc ccacggttct tgcttgatct ctgtgagggc gagtccgcgc tgcgtgagat
     5461 ccgcatggtc gcgctcgatg tcgctgcaat tgagcatgac tccttgcagg ccgccgggac
     5521 gcatgccgtc gaaccacatg accagggcga tcgtagtgga gcaccccttg ggcgcaagtt
     5581 gaatccactt ttggcccggg cccatcggct cttcgcgcag cagctcgaag cccagcacgt
     5641 cgcgataaaa ttgcttcgac ttttcctgat ccgacaccgg cactgaaacg atttcgaagg
     5701 aacgtatggt catgcgtcac tcctagaacg gatagttgat acctgtaaat gcagcgacgc
     5761 cctcgctgcc gtagccgatg tctacatatc cgaccacgaa ggggcgcgca attccgcgga
     5821 agccgacgcc gtagacatgg tgcagctgct cgaagggcag cgtgctggtg cgcgcaaaca
     5881 cccggccgac atcgatgaat ggcgtcacct ccacgtcgac gagggtggaa gtggcatcga
     5941 aggtaatcac cttgcgccgc agctccaccg tgctcgagaa ggagtcgcga tcgtagaagc
     6001 gcccggcacc gaagccgcgc agcggctgct cgccgcccac cacgctttct ccgccgccga
     6061 tgttgctcag cgcccaaaaa ggcacatcgt gcgaggtcgg catatagcgg agcgacatgt
     6121 gcccggcgag aatggtgtcc ttggcgagcg gccagaacgc gcgcccatcc ccgccgacct
     6181 cgctgtacat cgaatcgtcc aacagtccct tgcggctcgc caacccgcca tacgcgatca
     6241 attgcatccc gcggctgggc accaccaggt tatcgcgggt gtcatacacc aaggatacgc
     6301 ggttcagaat ctgcttattg gtgccgattc ccaacgagcg gctgaagcgg ctgccaatgg
     6361 ttgcaacctt ctcgagcgtg ccgggaagca catccacgtc ttcgaagcgc gcggtgtaga
     6421 gcagttgcca ggcgtggctg aaattcaggc cgacttgctg ctgcagggtt tcttgctggt
     6481 tggtgtagtt ggtctcggcg attgccggcg actcgttgcc gatgccgtag aagcgcggcg
     6541 tgccgtcgcg atcggaaatc acgcgggtgt tgaacgacca gcgatcttga cgcaaccggc
     6601 ccacctggta ctcggcttcg aattcgcgct cgacgtgctc ctggatgccg ctcaccaccg
     6661 accattgttc atcttccgat gtatagccgt agatacggcc gtgtacgccc actccgaaat
     6721 acgggttgta aataatgtcc ggtgcgatga tgcgccggat ttcgtgatta tcatcggtct
     6781 ttatccaggt cggaagcagg ccgagcgtcg tgccgctgtc cgggtcgacc gcgatttccg
     6841 gcaccggaat gaagggcgcg gtcccaggat tgaaccagcg cgacagaccc gtcgcgggcg
     6901 ccgcgggcgg cggcgcaatc ggtccggtag cgggcccttg tgcaggatcg gtgtcaggcg
     6961 gggccgcgta gcactcggtg cccaaggcga tgagcagcag ccacgccgcc ttcacagcag
     7021 cacgcggttg gccgatggcc ccgccagcaa cgtgctcaac atgcgtcgat cgtagagacc
     7081 gcgcccggcc gcaaggttga tcgcgcgcat gcataccctt cccgtcacga ggaagcgaga
     7141 atcttacacg gcggcagcag tgctgcgcga gcaaagctgc aggccgcgtc ccatggaaac
     7201 ttgttccggc gtcgagaaga tttaatcgat gggcttgccg cagcgagcca gcacgccgcg
     7261 cagatcgcga atcggaggga acacttcgtt gttccaatcc ccgccctggg cgtcatgggc
     7321 ccgctgctcg tattccacga tctccttgtc ctcgcggaaa atattttcgg tgaaccaggt
     7381 tacgaagggc cacaccgcat gaatgattcc cggcagcttg ggtttcttca ccgacagata
     7441 gccgtaggtg cgattggtgc gctgttctgc gtcgagcggc gtgtaaccca gccacacatc
     7501 gagcaccggg tcgtcggttt gctggatatc ccgccccacc caaactttca gactctgggt
     7561 cggatagtcg gtgcgaatcc gcatgtgatc gcaaaagtcc ttcttcgcct cgccgcgctt
     7621 gcgcatcaag tccacgatca cgtgttcgcc caccgaggat ttgccctcgg tgcggctgaa
     7681 gctatattcg acctgcgccc agttgtcgcc gtgtgcccgg cccaggcagc gcgcgctgat
     7741 cgagcccatc tgcttgcggt gcatgaactg gtggttcatg tcgaacagat tctcatgcat
     7801 gaatgtgtag tgacaggcca cctcgcgatt caagcgccga gtcttgtagt ccttgcgctg
     7861 cgaggagccg agattcgcgg gcagacgcgc atcggcaaag gcggcatcgc cggggaacac
     7921 gaaaatcagg ccgtcgactt cgcgcgcggg ataacttttc acgcccgtcg gcagccgctc
     7981 ggtgccgagg tagggcacgt tcacgcactt gccggtgccg ttgtagctcc agccgtggta
     8041 gtggcatttg agggcatcgc cgctcaccac cccgaggtgc agaggcacct ggcggtgcgc
     8101 gcagcgatcc tccaacgcga atacctgccc gctcttgccc cgatatagca cgatcggcag
     8161 cgccgcgaag cgccggccga gcgtcttgcc gactttcaat tcatgcgacc aggcgagagg
     8221 gtaccaatag tccggatgcg cacccacgcg ccgcaaatcg aacgcgttgg gtgaattcac
     8281 cggatgtagg ctgattaatt gcgagctgtc ttcggccatg gtcgtgttcc aattcgatgc
     8341 aggaatatta cccgatttgc gcaacgcctg accgctgccg ccgccgactg gaatcgcagc
     8401 gcgcgctgcc gcgcctgtgg cgggcctgca acgctcgggc atttgtcggc tgtcgcgtcg
     8461 tccccaattg cgacgaccct aaagccaatt caggcaatgt tgtggaaata actggaagat
     8521 tgccgcggtg cgagtcagaa tgcaaaaagc tcgcgacggc tgcggcgccg tcgcaacaaa
     8581 agatcagcga atctttgctg tagggaattg aagggagaac cagcatgcac accgcagcgg
     8641 atgcccagcg cgcagccaat cgatggcttg tcgaaatcaa cttcttgcgt gcgacccgga
     8701 tgctcgtgac ggcgagtgcc gcggccggcg tgatattcgc gacgcccgcc ggcgcgcaac
     8761 aggcgccctc ttccagttcg aaccagctgc aagagatcgt ggttaccgga tcgctgatca
     8821 agcgcaccga taccgagacg ccgagcccgg tgcaggtcat caccgccgag gacttgagca
     8881 atagcggatt caacaatgtc tctgacgtac tgcgcaatct tgccgccaac ggccaaggca
     8941 cgctcaatca aggcttcggc caggcattcg cttccggtgg ttccggcatc gcgctgcgcg
     9001 gcctgacggt gggcgacaca cttacgctga tcgacggcga acgcatggtc gcctatcctt
     9061 taagcgatga cggtgagcgc agcttcgtcg atatcagcgc gattcccttc aatgcgatcg
     9121 atacggtcga agtcctcaag gacggcgcct cggccctgta cggcgccgat gccatcgcgg
     9181 gcgtcgtcaa cgtcaaattg aaacagacct acgtcggatc ggagatcacg gccgagggcg
     9241 gtgagaccca acgccacgac ggccagactt accatgcgtc cgcgattcta ggctggggcg
     9301 atctcgccaa cgacggttac aacatttatg cggccctcga ctggcaccgc caggagacga
     9361 tattgggcag caatcgcagc ggcgcatgga ccaacctcga ctggagcgga ctgggcggcc
     9421 aaaacaccaa cggaggtgcc atcggtgcga ccggtctcgt ctatccggac agctcgaccg
     9481 ggtatctgct caatcccacg acgcctaatg cagccggcgt atatccgaat ggtctaccgg
     9541 gggaggtgtt cctgccgggc tgcaccgcga cgttgcaagc cgccgataag tgcaccttca
     9601 cgtatcccgg cgagattcag ccgccgaccg aacagaccaa cttcctcgcc aagttcacca
     9661 a
//
LOCUS       DASWZT010000454         6822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_85794, whole genome shotgun sequence.
ACCESSION   DASWZT010000454 DASWZT010000000
VERSION     DASWZT010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6822
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85794"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..629)
                     /locus_tag="VGE92_12875"
     CDS             complement(228..629)
                     /locus_tag="VGE92_12875"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY1314768.1"
                     /translation="MTEASRGVLDTSVLIDHDQITAEQLPDESAITAVTMAELAAGPH
                     ATENKDERARRQDRLQWATATWDPLPFDAESARMYGRVFAAARAAGQSSRARLADLLI
                     ASTAAASGLPLYTRNPSDFAALKRILKVLKV"
     gene            complement(622..873)
                     /locus_tag="VGE92_12880"
     CDS             complement(622..873)
                     /locus_tag="VGE92_12880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEY1314769.1"
                     /translation="MKIRTISQRELRNQSAAVLREVEAGRTIIVSRNGTPVGELRPIK
                     RRRFVPRAAIADAAGNAARIDAAQFRADLDAVVDPRPHD"
     gene            complement(953..2212)
                     /locus_tag="VGE92_12885"
     CDS             complement(953..2212)
                     /locus_tag="VGE92_12885"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY1314770.1"
                     /translation="MASGKLTSLSIERAHRFGGPVLLLSDGLYFRKQTREGAAWMIRY
                     RFAGRERWMTLGNFPDMSLATARIEAREKRLMLDKRRDPLFAKQAIEEAQRAAFAAQK
                     ARQTFRDLAEDWYGTEIDQRRLKHPKVPRRYLDKYLLPAFGERVPAEITAADAARLLA
                     QVRKTAPTAANDLLRFMRRVFRFGVRRHVLTSNPVADFDQSDAGGPERARKRALGVEE
                     LGQLFKALRDSASFGGANLLAVKLLLALGVRKGELLGAQWKEFDLNGAAPTWRLPARR
                     TKTGEALTIPLVPTVASWLKSLREVAGSSEFVFPKRRRDPRQRVLHLGIDTLNAALAA
                     VEHGLEHFTLHDLRRTMRTHLASLGVRSEVAERCLGHKLRGVEGTYNTHDYLNERRAA
                     LETWTALLLDIESGAQKVTPIRRKAAR"
     gene            complement(2419..3282)
                     /locus_tag="VGE92_12890"
     CDS             complement(2419..3282)
                     /locus_tag="VGE92_12890"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEY1314771.1"
                     /translation="MIRSMTGFARRERQFPWGSLAWELKTVNHRFLEMGLRLPEEFRV
                     GEAEFRQAIAACVRRGKVDCSLHFRAAASGSALEVDADLLGSLLQRVQQIATQAGAAA
                     RIDTLDLLRWPGVIRDAGRDHTPMIMAAHALLAEALSELTRFRDSEGGRLRDALEQRC
                     AGLVEFAARVTDRLPEVRSRMRAKLLDRIAQLVSDVDHERLEQELAILAQRLDVDEEL
                     DRLRGHLAEVRKTFVGQEAAGRRLDFLMQELNREANTLSSKSQDIETTRAAVDMKVLI
                     EQMREQVQNIE"
     gene            3344..4090
                     /locus_tag="VGE92_12895"
     CDS             3344..4090
                     /locus_tag="VGE92_12895"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEY1314772.1"
                     /translation="MQIETADVSLIGHREENQDRVAVAAAEQAVLLVVIDGMGGHAEG
                     ARAAETALATLLESFWQTPHPIFDPLGFLHLSLGRAHEEVVKLGNGLSVEARPRATCA
                     LCLVQDSAAYWAHVGDSRVYQLRQAEVLERTRDHSHVEVLLREGLITEAEVHGHPMRN
                     YVECCLGGDAALPEMSISTRRKLKGGDVLLLCTDGLWANLRDQDFVRFAQAAGKPLRE
                     SLTDLGTQAVEASAPYSDNTSAAALRWRKA"
     gene            4093..4809
                     /gene="rph"
                     /locus_tag="VGE92_12900"
     CDS             4093..4809
                     /gene="rph"
                     /locus_tag="VGE92_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEY1314773.1"
                     /translation="MRPSGRAPDEMRAVSFERGYTKHAEGSVLVGFGETRVLCTASIE
                     DSVPSFLRGQAQGWVTAEYGMLPRATHTRSGREAARGKQSGRTQEIQRLIGRALRAVV
                     DLKGLGERTVTVDCDVLQADGGTRTAAITGGFVALADAIDTLLKRRAVAANPLHGQVA
                     AVSVGIYRGVPVLDLDYAEDSNAETDMNVVMNSGGAFVEVQGTAEGHAFRRHELDQLL
                     DLASAGIARLHAAQQSVRAA"
     gene            4778..5416
                     /gene="rdgB"
                     /locus_tag="VGE92_12905"
     CDS             4778..5416
                     /gene="rdgB"
                     /locus_tag="VGE92_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEY1314774.1"
                     /translation="MPLNRAYVPRRPLIVATSNPGKLREFRALLADLPFDLRSLGELG
                     VAPPQETGASFLENALQKARHAAAAAPGAAAIADDSGLEVDALGGAPGIFSARYAGAG
                     ADDAANNAKLLRALAHAPGTLRSARYRCVLAYVAGAEDPQPLLAHGTWEGSILDVPRG
                     SGGFGYDPYFWLPELQQSAAQLDLAHKNRISHRGIALRALREQLLAQCAARP"
     gene            5413..6588
                     /gene="hemW"
                     /locus_tag="VGE92_12910"
     CDS             5413..6588
                     /gene="hemW"
                     /locus_tag="VGE92_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEY1314775.1"
                     /translation="MSERSLALYVHMPWCVRKCPYCDFNSHQLKSAAPDAAYIDALLR
                     DFELELPRLEQRRVDSVFFGGGTPSLFQPEDFSRLLGALRQRIAVAADVEVTLEANPG
                     TIERGRFAGYRDAGINRVSLGAQTFAPRALEALGRIHSADDTKRAVAELRAAKLDNFN
                     LDLMYALPQQTLEEALEDIRIACALAPAHLSHYQLTLEPGTVFHSRPPPLPDEDAAWQ
                     MQCLSQKLLADAGYAQYEVSAYARAGARCRHNLNYWRFGDYVGIGAGAHGKITLALPQ
                     RILRTSKPKQPRDYQDQVRRAQQGASEAAPDSALGASSFIALEDVPFEFMLNALRLND
                     GFTGREFRLNTGLELESVEPKLVAAEGRGLLCRAGDQWRASELGRRFLNDLQLSFLP"
BASE COUNT         1238 a         2160 c         2183 g         1241 t
ORIGIN      
        1 ggtcggaata agaattgatg cagccaagaa gcgggtatga gaggtcatca ctgtagaaat
       61 ccattgcccg ttggttgtta cgagccttac ttcaacaaat tccccctatg ccgcgttagc
      121 cgacagctag cgtatgcacc gtcgcgccgt gcgcggcgat cccaaggttc aagagcggac
      181 gctgtaggtc agcgggccgc cttccgcgaa ccccgctgag caggcaacta gaccttgagg
      241 accttgagga tgcgctttag agcggcgaaa tcagagggat tgcgtgtgta gagtggcaat
      301 ccacttgccg cagccgtcga ggcaatgagc aagtccgcga ggcgtgccct gctggattgt
      361 ccggctgccc gcgcggcagc aaagacccga ccgtacatac gtgccgattc cgcatcgaaa
      421 ggcaacggat cccaggtggc tgtcgcccac tgcaaacgat cctggcgtcg ggcgcgctcg
      481 tctttatttt cggttgcatg tgggcctgca gcgagttccg ccatcgtcac cgcagtaatc
      541 gcggattcat caggaagttg ctcagctgtg atctggtcgt gatcgatcag aaccgacgtg
      601 tccaatacac cgcgactcgc ctcagtcatg ggggcgtgga tcaactacgg catcgagatc
      661 tgcacggaac tgcgcggcat cgatccgggc ggcattgcct gcagcatccg cgatggccgc
      721 gcgaggcacg aagcgacgtc gcttaatggg tcgtagttct ccgaccggcg tgccatttcg
      781 gctaacgatg atcgttcgtc cggcctccac ttcacggagc actgcggcgg attgattgcg
      841 aagctcgcgc tgactaatgg tgcggatttt cataaaccgg actgtagcac actgtagcat
      901 actgtagcaa gcgtgccgga cgataccttg tcggcattgc cggccgcgga tctcagcggg
      961 ccgccttgcg tctgatgggc gtgaccttct gcgcgccgct ttcgatgtcg agcaacagtg
     1021 ccgtccaggt ttccagcgcc gcgcgacgct cgttgagata atcgtgggtg ttgtaggtgc
     1081 cctcgactcc gcgcagcttg tgacccagac agcgttcggc cacttcgctg cgtacgccga
     1141 gggaggccaa atgcgttcgc atggtgcggc gcaagtcgtg caaggtgaaa tgctccagtc
     1201 cgtgctcgac cgcggcaagc gccgcattca acgtatcgat acccagatgc agcacccgct
     1261 ggcgcgggtc gcgccggcgc ttcggaaaga cgaactcgct actgcctgca acctcgcgca
     1321 acgatttgag ccaggatgca acagtgggca ccagcggaat cgtcagcgcc tcgcccgttt
     1381 tggtccgtct ggccggcaac cgccaggttg gcgctgcacc gttgagatcg aactccttcc
     1441 attgggcgcc caataattcg cccttgcgaa cgcccagggc gagcagcagc ttcacggcga
     1501 gcagattggc tccgccgaag ctcgcggaat cgcgcagcgc tttgaagagt tggcccagct
     1561 cctccacgcc cagggcccgc ttcctcgcac gttcggggcc gcccgcatcg ctttggtcga
     1621 agtccgccac gggattgctg gtcagcacat gacggcgcac gccgaagcgg aacacgcgcc
     1681 gcatgaagcg cagcagatcg ttcgccgcgg tgggcgcggt cttgcgtact tgcgcgagga
     1741 gtcgagcggc atcagccgcg gtgatttcgg cgggcacgcg ctcgccgaac gccggcagca
     1801 ggtacttgtc gaggtaacgc cgcggcacct tcgggtgctt gaggcgccgc tgatcgatct
     1861 ccgtgccgta ccaatcctcc gccagatcgc gaaacgtttg gcgcgccttt tgggcggcga
     1921 acgcggcccg ctgcgcctct tcgatcgctt gcttggcgaa cagcggatca cgccgcttgt
     1981 cgagcataag gcgcttctct cgcgcctcga tccgcgccgt ggcgaggctc atgtcgggga
     2041 agttgccgag cgtcatccag cgttcgcggc cggcgaagcg atagcggatc atccacgcgg
     2101 caccctcgcg tgtctgcttc ctgaagtaca ggccgtcgct gagcagcagc accgggccgc
     2161 caaagcggtg tgcgcgttcg atcgagaggg aggtgagttt tccggacgcc atgtggagac
     2221 cccggatgcg cagttggatc tcgtacaagc cgcgtagcta gaaaatctag ctacgcattt
     2281 agctacgcac ctagccacaa cagtagcccc gacgagggaa aacgtccaga gacaataatt
     2341 gacgcaaagc cctagttttg caggggtttc atgcgctttt cgaggacaac cggcgacagc
     2401 cgcagacagc cgaaaacgct actcgatgtt ctgcacttgt tcgcgcatct gctcgatcag
     2461 caccttcata tcgacggcgg cgcgggtggt ctctatgtct tgagatttcg atgacagagt
     2521 attggcctcg cgattgagct cctgcatgag gaaatccaag cgccggccgg cggcttcctg
     2581 cccgacgaat gtcttgcgca cttcggcgag atggccgcgc agccggtcga gctcctcgtc
     2641 gacatcgagc cgctgcgcga gaatcgccag ttcctgttcg agccgttcgt ggtcgacgtc
     2701 gctcaccagc tgagcgatgc ggtcgagcag tttcgcgcgc atgcgcgagc gcacttccgg
     2761 caaccggtcg gtcacgcgtg cggcaaattc gaccaacccc gcgcagcgct gctcgagggc
     2821 gtcgcgcagg cgcccgcctt cgctgtcgcg aaagcgcgtc agctccgaca acgcctcggc
     2881 caagagcgcg tgcgcggcca tgatcatggg agtgtgatcg cggccggcgt cgcggatcac
     2941 gccgggccag cgcagcaaat ccagggtgtc gatccgcgcc gccgcccccg cctgcgtcgc
     3001 aatctgctgc acccgttgca acaggctgcc gagcagatcg gcatcgactt cgagcgcgct
     3061 gccactcgcg gccgcgcgaa aatgcaggga acaatcgacc ttgccgcgac gcacgcacgc
     3121 ggcgatggcc tgtcgaaatt cggcttcgcc gacccggaat tcctcgggca accttaagcc
     3181 catttccagg aaacgatgat tgaccgtctt caattcccag gccagcgagc cccacgggaa
     3241 ctggcgctcg cgtcgcgcaa atccggtcat gctacgaatc ataggggtct gcgctaaaat
     3301 cggccataat cactgctttc agtttatcac ttaggagcag tcattgcaga tcgagaccgc
     3361 cgacgtcagc ctcatcggtc accgcgaaga gaatcaggac cgagttgccg tcgcggctgc
     3421 cgagcaagcg gtgctgctgg tggtgatcga tggcatgggc ggccacgcgg aaggcgcacg
     3481 cgccgccgag actgctctgg ccaccttgct cgaatcgttc tggcagacgc cgcatcctat
     3541 tttcgatccg ctggggttcc tgcatttgtc gctcggccgc gcccatgaag aagtcgtgaa
     3601 gctcggcaac ggcctgagtg tcgaagcgcg gccgcgcgcg acctgcgcgc tgtgcttggt
     3661 tcaagactcg gcagcttact gggcgcatgt cggcgatagc cgcgtctatc aactacgtca
     3721 agccgaagta ctggagcgca ctcgcgatca cagccacgtc gaagtgctgt tgcgcgaagg
     3781 cttgatcacc gaggcggaag ttcacggcca tccgatgcgc aattacgtcg agtgttgtct
     3841 cggcggagac gcggccttgc ccgagatgag catatcgacg cggcgcaagc tcaaaggcgg
     3901 cgacgtcttg ctgctgtgta ccgacggcct gtgggccaat ctgcgggatc aggatttcgt
     3961 gcgctttgca caggccgccg gcaagccgtt gcgcgaatcc ctgacggatc tcggaaccca
     4021 ggcggtcgag gcgtccgccc cctatagcga caacaccagt gcggcggcgc tgcgctggcg
     4081 gaaggcgtga gcatccggcc gagcggccgc gcgcccgatg agatgcgcgc cgtgagtttc
     4141 gagcgcggct acaccaaaca cgccgagggt tcggtgttgg ttggcttcgg cgagacgcgc
     4201 gtgctgtgca cggcaagcat cgaagatagc gtaccgtcat ttctgcgcgg ccaggcgcaa
     4261 ggttgggtga ccgcagaata cggcatgctg ccgcgggcca cgcatactcg ttccggccgc
     4321 gaggcggcgc gcggcaagca gagcggccgc acccaggaga tccagcgtct gatcggccgc
     4381 gccctgcggg cggtggtcga cctcaaggga ttgggcgagc gcaccgtgac cgtcgactgc
     4441 gacgtgctgc aggcggatgg cggcacgcgc acggcggcga ttaccggcgg gttcgttgcc
     4501 ctggcggatg cgatcgacac gctgctcaag cggcgcgccg ttgccgcgaa tccgctgcac
     4561 ggtcaagtcg cggcggtgtc cgtcggcatc tatcgcggcg tgcccgtgct cgacttggac
     4621 tacgccgagg attcgaacgc ggagaccgac atgaacgtcg tcatgaacag cggcggcgca
     4681 ttcgtcgaag tgcagggcac cgccgagggc cacgcgttcc gccgccacga gctcgaccaa
     4741 ttgctcgacc tcgcgagcgc ggggatcgcg cgcctgcatg ccgctcaaca gagcgtacgt
     4801 gccgcgtagg ccgctcattg tcgcaaccag caatccgggc aagctgcgcg aattccgagc
     4861 actcctggcc gatctgcctt tcgatcttcg cagtctcggc gaattggggg tagcgccgcc
     4921 gcaggagacc ggcgcgagct ttctggagaa cgcgctgcaa aaggcgcgcc acgcagcggc
     4981 ggcggcgccg ggtgcggcgg cgatcgccga tgactcgggg ctcgaggtcg acgccctggg
     5041 cggcgcgcca ggcatttttt cggcgcgcta tgccggagcg ggcgccgatg atgcggcgaa
     5101 caacgccaaa ttgctgcgcg cgctcgcaca cgcgccgggc accctgcgca gcgcgcgtta
     5161 tcgctgcgtc ttggcttacg ttgccggcgc cgaggatccg cagccgctgc tcgcgcacgg
     5221 aacctgggaa ggatcgattc tcgatgtgcc gcgcggcagc ggcgggttcg gctacgatcc
     5281 ctacttctgg ctgccggagt tgcagcagag cgccgcgcag ctcgatttgg cacacaagaa
     5341 ccggatcagt catcgcggca tcgcactgcg cgccttgcgc gagcagctgc tggcgcagtg
     5401 cgcggcacgg ccatgagcga acgatcgctg gcgttgtacg tgcacatgcc ctggtgcgtg
     5461 cgcaaatgcc cgtattgtga tttcaactcg catcaattga agtcggccgc gcccgacgcc
     5521 gcatacatcg atgcactgct tcgagatttc gagctcgaac tgccgcgcct cgagcagcgc
     5581 agagtcgaca gcgtgttctt cggcggcggc acaccgagtc tgtttcagcc cgaggacttc
     5641 agccgcttgc tcggcgcatt gcggcagcgg atcgcagtcg ccgccgacgt ggaagtgacg
     5701 ctggaagcga acccgggcac catcgagcgc ggccgcttcg ccggctatcg cgacgccgga
     5761 atcaatcgag tgtcgctcgg cgcgcagacc ttcgcgccgc gcgccctcga agcgctcggc
     5821 cgcattcatt cggccgacga tactaagcgc gcggtcgcgg aattgcgcgc cgccaagctc
     5881 gataatttca atctcgactt gatgtacgcg ctgccgcaac agaccctcga ggaggcgctc
     5941 gaggacatcc gcatcgcgtg cgctttggcg cccgcgcacc tgtcgcacta tcagctcacg
     6001 ctcgaaccgg ggaccgtgtt tcactcgcgg ccgccgccgt tgcctgacga agatgccgct
     6061 tggcagatgc aatgcttgag tcagaagttg ttggccgatg ccggctacgc gcaatacgag
     6121 gtgtccgcgt acgcgcgcgc cggcgcgcgc tgccggcaca atctcaatta ttggcggttc
     6181 ggcgactacg tcgggatcgg cgccggcgcg cacggaaaaa tcactctggc gcttccgcaa
     6241 cgcatcctgc gaaccagtaa acccaagcag ccgcgcgact atcaggatca ggtgcgccgc
     6301 gcgcagcagg gagcaagcga ggcggcgccg gatagcgcac tcggcgccag ttcgttcatt
     6361 gcgctcgagg atgtgccgtt cgagttcatg ctgaatgcgc tgcggctgaa tgacggcttc
     6421 acgggccgag aatttcggct gaacaccggc ctcgagctcg agtcggtcga gccaaaactc
     6481 gttgcggcag aaggacgtgg cctattgtgc cgcgctggcg accaatggcg cgccagcgag
     6541 ctggggcgtc gcttcttaaa tgatctgcag ctaagttttc ttccgtgaag cttatgcaca
     6601 cggcgaccgg gaactcgcac aaaggggtac ctacaacaca ttgcgcctgc agtaggtcag
     6661 atgcacctct taacctattg aataataagc agtaagtgac ttgtctattt tttgaccaaa
     6721 ttaacaatta gtcgaatttt cagccgtgcg aatcgaggca cctcggcgcc gtccacagac
     6781 ttatccacag tttttgtgga caacccgcaa gcggttgtga ca
//
LOCUS       DASWZT010000455        19904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_95677, whole genome shotgun sequence.
ACCESSION   DASWZT010000455 DASWZT010000000
VERSION     DASWZT010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 19904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19904
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95677"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VGE92_12915"
     CDS             <1..192
                     /locus_tag="VGE92_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314776.1"
                     /translation="VELEPRAGKPGTRAFEVFLKCFERGVMVRQTGDTIAMSPPLIID
                     SKQITRMVETLGAVIRETA"
     gene            complement(205..1707)
                     /locus_tag="VGE92_12920"
     CDS             complement(205..1707)
                     /locus_tag="VGE92_12920"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY1314777.1"
                     /translation="MIRYATRALMMFCMAAGARGEVPVAAAPGAPGIAWFQGDVAEAF
                     KLAQTEKKPVFLYWGAKWCPPCQQLKATVFSRKDFIAKSREFIDVYLDGDAQGSQKWG
                     DKFGASGYPTVVILRPDQKEITRISGGMDLSVYAGLLDAALNDLRPMDDVIAAMRSGT
                     AESSDDCRRLAYYGWDLRDFSQVERKSLARGLARAVTPCGGAKSLEGARIAIASAVFA
                     PTASNVTQVIAIVENPSIAARAADSLENLGSPFYKAVYARGPEVREVFMHAWSGTMEG
                     VAADPAVTDADQLSALGTRLALLKEFAADNKVPAAIAAAARIRTNATLAKKFDPYVRA
                     GVVNAASDVYEQLGDLDAQYALVSGELSTSHTPYYYMIDLGEIEESRHHTTQALAWYE
                     RAYRESEGIATRFQWGAQYLSALLRLAPQDHVRIRDAGMAVIGELDGPDRIQARTRYG
                     LGKLDAKLKKWNSAHHYDADIHALRSRISATCAKLPPSDSGRGSCRKFLS"
     gene            complement(1724..2086)
                     /locus_tag="VGE92_12925"
     CDS             complement(1724..2086)
                     /locus_tag="VGE92_12925"
                     /inference="COORDINATES: protein motif:HMM:NF017690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY1314778.1"
                     /translation="MSVHPKIVVIDRLQTGESANPSPEKILAGIPRTRVSNQYHEPSQ
                     QFFCGIWSSTAGKWRVSYTEHEFCVLIEGRVRIESASGEKLELRAGDAFVVPAGFEGV
                     WEVTEPCKKWYAIFEPKT"
     gene            complement(2180..2959)
                     /locus_tag="VGE92_12930"
     CDS             complement(2180..2959)
                     /locus_tag="VGE92_12930"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY1314779.1"
                     /translation="MNTVFSRTVRTIGLLAISVAANAGEPKPATPQEPIGFVTGGVIG
                     AFAAGPVGAVIGAGLGTWLGNRVHRAGDAARAEATVAALQTDKLELQTEKSTLADEKT
                     ALTQSNRSLSARLDDLSHQVESAQNAAKGDGSDVLDGLQGDVLFRTGSAEITPEIAHQ
                     IQVLAEAVAKSPALKVRVDGYADPRGTVDSNLKLSEARANAVRDLFLAAGIKEEVLEV
                     NAYGKSQSVAADNDGYALERRVRLTLQAQSEAAVAQVGENE"
     gene            complement(3108..3818)
                     /locus_tag="VGE92_12935"
     CDS             complement(3108..3818)
                     /locus_tag="VGE92_12935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEY1314780.1"
                     /translation="MTTAPQTRQYRYYDFVMAAYVCILLCANLIGPAKETSIQVPVFG
                     TLTFLAGVLFFPISYIFGDILTEVYGYGRDRRVVWAGFAALAFASLMSAVVVHLPPSE
                     SSRGTQAAVEAVFGNTPRIVAASIVAFLCGTFVNSYVLAKMKIWTRGRWLWTRIVGST
                     LCGELVDSVIFYFVAFYGRMPLAHLIAITFTQYALKSGWEFVATPITYRVVAFLKKAE
                     NEDYYDTDTNFTPFSLKA"
     gene            3957..5258
                     /locus_tag="VGE92_12940"
     CDS             3957..5258
                     /locus_tag="VGE92_12940"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY1314781.1"
                     /translation="MENGRPFGATDSPLVDFDAVCQKVRAAVEPARAHAVSLHDEQGE
                     VLWLSESSMGPDEHNAVREAVDKFSSPGSPPLLVADLGDSRAALLLRAVNGRRAMVGA
                     IMIVMDARAVKQDARGALRIMTPKLQSALADFAEMRPGPAPARPAGPRPLASLGSPAG
                     LTAKRPQAAVSPEIDRLHAALRRSPIILHVQRLVPLTKGSQLKRFEVLLRSKSEEAPH
                     AAPQAMLKAAVEHGLGSMIDRRVLTELIGFLVHHPEVWKSSAVMFSVNLTTTALHDEH
                     FIKFVELCLAKSSLPKGMIAFEVDTSTAVKLAAKMTEVAAALHRLGCPLVLDDFQLST
                     ECFALLRLPGVRYVKLAPEITSKMRTDKLAQAAITAVVQMARVLGMHTVAKRTETAAE
                     QEWLTALGVDFVQSNSMSPAVAIESLAKTSNPPKPPRIPTV"
     gene            complement(5255..6730)
                     /locus_tag="VGE92_12945"
     CDS             complement(5255..6730)
                     /locus_tag="VGE92_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY1314782.1"
                     /translation="MADESRRTLRRLATWWSAVLAASGCAVGPDYHKPSVDTPPSWQP
                     PAPWHAAAPADGALKGEWWRLFHDDALNPLVERALNSNQNIKVAAARLDQARDQVTVA
                     ASALYPSVDLSAGAARGKTSANRPLAAYSEPNQSTVQNDFTLGPAVNYEADLFGRIRR
                     QVEGARASAQQADADFENTRLILIAQLATDYFALRELDAEVDVVRNSLGLERDALGFI
                     GSRHDLGFATGLDLAQQRALVDSSATQLELLENQRAQYEHAIATLVGTPAPNFALPAA
                     VATASLPSIPPGMPADLLQRRPDVASAERAMAAANARIGVARAAYYPSIELGSGFGSP
                     NAGWQSNALATLFAAPSRLWSLGLVATQTLFDAGKTRANVRIADADYSAAVASYRQTV
                     LTAMEEVENGITGLASLDRAATQADSGVKNAQQAFDIATTRYKGGVDTYLEMITAQQV
                     LLGNQRQAVQVHGQQFTTAVYLVKALGGGWSADAARLSRNN"
     gene            complement(6732..7892)
                     /locus_tag="VGE92_12950"
     CDS             complement(6732..7892)
                     /locus_tag="VGE92_12950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY1314783.1"
                     /translation="MPEAETFSVVKRAKWVAGAILGVLLIGAVIVLVMRSFQASALES
                     STALHAKQYVTTILPKTGGDAQPLTLPGTLLGVIESTVYARSNGYVVRWVKDIGSSVK
                     KGDLLAEIAAPEIDQELSQAVSSRAQADASASLAKSTADRWQALRQKDAVTQQDLDER
                     LSAFNQAAANLAAADANVGRLQKLQGFNRVLAPFDGVVTRRNIDVGDLVNAGNGGTGQ
                     ALFSIAQVDPMRLYIYVPQMYARQIKIGDELTVTLAERAAEQYRGTVARTARAIDTAT
                     RTMQVEIQIPNPTGALIAGSYVQVTLPINADARALVVPTNVLLFRPEGTRVALVDSGG
                     RVHLAAVKLGTDFGADVEVLSGLNAADRIVLNPADSLAEGDVVTLPAPAKGE"
     gene            complement(7894..11079)
                     /locus_tag="VGE92_12955"
     CDS             complement(7894..11079)
                     /locus_tag="VGE92_12955"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1314784.1"
                     /translation="MWIVEVALRRPYTFLVMALLILLATPLVLLKMPTDIFPEINIPV
                     ISIVWTYTGLSAQEIGQRITSVNERSLTTTVNDIEHIESESLAGFAVIKIFLQPNANI
                     PTAIAQVVAIEQSQLKQLPPGILPPLVIKYSASSIPIIQLGVSSPSMTEQAVFDTAVN
                     FLRPHLVTIPGVAIPYPYGGKQRVISVDLDTDALLAKGLTPNDVVNAVNTQNLVLPSG
                     TAKIGATEYVLATNGSPDTIAGLNNIPVLTRNGATTYLSEVAHVRDGFSPQTNIVRQN
                     GERGVLVSVIKNGGSSTLDIVSSLLAQLPIVAQILPKELKITPLFDQSGFVRAAISGV
                     VREALIAACLTAALILLFLGNWRSTCIIAVSIPLSILSSIIALYAMGETMNIMTLGGL
                     ALAVGILVDDATVTIENIERHMHLGSDLHKAILEGAGEIAVPALVSTLCICIVFVPMF
                     FLTGVARYLFAPLAQAVVFAMLASYVLSRTLVPTLVMLLMDHTARQSGASVLQRVYRA
                     FNGRFERIRSGYVIILSQVLQRRRVFAGAFLGFCVLSCCLAPFLGRDFFPSVDGGQIR
                     LHMRLPTGARIEETARVADAVERVIRSIIPPKDLGTILDNLGVAVSGINKSYSNAGTF
                     GSHDGEIQVSLNVGHRPTERYVEAMRRELPQRFPGVEFFFQPADMVTQILNFGLPSAI
                     DVKVTGADLRADYGIAAKLMKQIEMIPGTVDTHIHQRLDQPTVSLNMDRTQLQQLGLT
                     PSDVTTDLLVSTAGTTQTSPAFWLSPVNGIVYSLAVQSPQYAVDSLDAVLRTPVRAAA
                     AALSTTPQLLSNLVGVTPAVQEAVDSRYNLAPTIDIYSSAQGRDMGGISVDIRRLLDE
                     LRPHLPRGVSVEMRGQTETMKSSFVELGVGLVMAVVMVYLLIVVNFQSWVDAFIIITA
                     LPAALAGICWMLFLTGTTLSVPALTGAIMTMGVATANSILVVAFARQRMGEGLDPVKA
                     ALDSGATRMRPVMMTALAMIIGMVPMALGLGEGAEQNAPLGRAVIGGLLFATVSTLLF
                     VPVIFAGIHQRLESRKAERRSAVPPLAEGST"
     gene            11376..12023
                     /locus_tag="VGE92_12960"
     CDS             11376..12023
                     /locus_tag="VGE92_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314785.1"
                     /translation="MTLIALIIVALAGILFLFAAMRRLRQRKVISGLFHGATALVLIL
                     VGVGAALLLTNLRTYQRLSFEQRAGELQLTRTGDRQFNAVLSYPNGERANFALRGDEW
                     QVDARVLKWHAFANLVGFDTAYRLERLSGRYSRVEDEVAQPRTAFSLNPPQHIDPWVL
                     VHRYRSWVPWIDAVYGSAAFLPMADGALYEISVSQSGLIARPLNQAARDAVGGWH"
     gene            12023..12889
                     /locus_tag="VGE92_12965"
     CDS             12023..12889
                     /locus_tag="VGE92_12965"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY1314786.1"
                     /translation="MVRRVARFALIAGAAVVLLMALAMAWLKIHETELVFRGARSREH
                     LLKALPAEAERVAVDEPGGIKLAGTVFHAAAADTAFWVLHLHGNADSAFSPWQVRHCK
                     ALRDAGFNVLDIDYRGFGETPGEPSEAAMYEDSEAAYQELLRRGVPQRRIIVLGHSLG
                     SGPAVFLATRHQAAALVLFGAFTSIPDAAAGQYPYLPVRLAVGVQFNSFARIGEVHIP
                     VVITHSRGDTLIPYSHALKLLAAANEPKRLLTFDSATDGFGGHVDALYEHVDILRATL
                     ASLIPSMQPPPQ"
     gene            12889..14736
                     /locus_tag="VGE92_12970"
     CDS             12889..14736
                     /locus_tag="VGE92_12970"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY1314787.1"
                     /translation="MTVAQKLSTLLLTLLLALAGFAWWKTAPPTAKVTRQRAAALAAA
                     ELVDQSTYNTAQRLAQLADTPEEQAYAQAALRVADHALAFAFTTALRDVEAHPPPLSP
                     EALQIQDRIRKSQVLLDTDQQRVTELAGTLAQAKAAQKDAVQDELDLAQSQLDLDKDE
                     LEEANQDLIEAGGNQQKRIEALQQEHEAQVKARAAPPAAGAASAGTAAFEKHGAFGRL
                     IEWRHLWLKQQELFDAQSSAAQKAANLTGRRTALAAALEKAKSGITELAQHTKSAKQE
                     GTAAAPQVRIQHTHEDAAALLNQTKLIAADQKVLTLLDRRTADQKQLDSIYGQWSALV
                     TLQAHTLAHSLLLSALIVIAILILVMLLDGWTQRLLDNPKLDRRQVETLRSIARVTLR
                     VIAVVIILLIVIGLPTQFGTMIGIVGAGLTVALKDFIVAFFGWLMLMGRNGIRLGDWV
                     EINGVSGEVAELGMFHTVLMETGNWSDAGHPTGRRVTFTNSFAIEGHYFNFSTSGQWL
                     WDELQVVVPAGRDPYPIVDAITKQVVEATAESARQAEQEWQRAVPAQRGKVFSGTPGV
                     NVKPVVGGIEIAIRYITRANERFLLRAKLYQAAVDLLGDKYSAQKPTTV"
     gene            14751..14825
                     /locus_tag="VGE92_12975"
     tRNA            14751..14825
                     /locus_tag="VGE92_12975"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14783..14785,aa:Thr,seq:tgt)
     gene            15140..15874
                     /locus_tag="VGE92_12980"
                     /pseudo
     CDS             15140..15874
                     /locus_tag="VGE92_12980"
                     /inference="COORDINATES: protein motif:HMM:NF024750.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            16282..16590
                     /locus_tag="VGE92_12985"
     CDS             16282..16590
                     /locus_tag="VGE92_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEY1314788.1"
                     /translation="MSKKFVKRAVDLANLPSLTKRQKADLAALAARPDGEIDFSDIPP
                     LTEDFWKNAARGKYYKPTKTSTTVRIDSDVLAWLRSQGKGYQSRINAILRRKMLESLK
                     "
     gene            16604..16942
                     /locus_tag="VGE92_12990"
     CDS             16604..16942
                     /locus_tag="VGE92_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314789.1"
                     /translation="MPIDIDALTEPELIDLNHRIVARLRFLRQIDAHATMLEFRIGER
                     VTFRPDGRPPVTGMITRYNKKTVSIVTNDGQRWNVAPHFLECAAPRATSKPGGDNVIS
                     IQRNDKHSNK"
     gene            complement(17174..18082)
                     /locus_tag="VGE92_12995"
     CDS             complement(17174..18082)
                     /locus_tag="VGE92_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1314790.1"
                     /translation="MEIVQRLFSWQLSVLLFAGITAGAQVNNSNQGGQSPDSPPAENA
                     TPPGDGQATTTQPTISPGITVTGTTPRAEPPPPKLAPDQFTNCYAMSKSTDPEQMDFV
                     AMETCEAQLAVDQRNVINKCINRDGKSAPPVIIQACTELLDRNILQGHERFYLFVNRA
                     VAYVAQGDKQHALDDYNTGVKLAPKYAQPYYYRGVFYATQTDVDAALRDLGTALSLNP
                     KLVPALLQRARIHQTQHAIKDEAQAIRLDPKMARAYFLRGAAFGDLGDSPNAVSDVTT
                     AVELDPSLDRYISSNGKTATLTLPPL"
     gene            complement(18319..19599)
                     /locus_tag="VGE92_13000"
     CDS             complement(18319..19599)
                     /locus_tag="VGE92_13000"
                     /inference="COORDINATES: protein motif:HMM:NF016150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HEY1314791.1"
                     /translation="MPALSKSTNPGFVTTATPPRDRIDSIDALRGIALFGVLAVNLVM
                     EFRVSIFQQFIAGSTPATGVNGFVEDFVSFALESKAFCVFSFLFGVGLAMQLERLSRR
                     PAPYLLLTRRLVALLGFGLIHLIFIWNGDILTEYALAGLLILPFLRLPNWALAVAAAG
                     FFTLYALMPSLPPLVAWPDTSAFASDVATADRVYSAGFYLEGWRFSVHELPLLVPLHV
                     FVFPRTVALFLFGAFVWRMGVLRQPSSHSRLFSVVAAAGLGVGMALTYGATAGAFNSW
                     GTLGMVLQALAPVVLAVGYGSVVIYLAQLHGAHNVLRPFAAVGRMAFTNYIAQSLIFG
                     FVFFGYGLGLFGKMNAAPALLFGVIVYLLQAVLSAAWLRRFRFGPLEWFWRSLMYGRA
                     QPMLMAVYARSAAEAYFRAGQPFERPLCASNLLR"
BASE COUNT         4036 a         6077 c         5983 g         3808 t
ORIGIN      
        1 gtagaactcg aaccgcgagc cggcaaaccc gggacgcgcg ccttcgaagt gttcctcaag
       61 tgcttcgaac gcggggtgat ggtgcgccaa accggcgata cgattgccat gtcgccgccc
      121 ttgatcatcg actccaagca gatcacccgt atggtcgaaa ccttgggcgc ggtgattcgc
      181 gagaccgcct agaacgcaac atcgctagct caggaatttc ctacagctcc cgcggccgct
      241 gtcgctcggc gggagtttgg cgcaggtggc gcttatgcgg gagcggagcg cgtggatgtc
      301 ggcgtcgtaa tggtgagctg aattccactt cttgagcttg gcgtcgagtt ttcccaaacc
      361 gtagcgcgta cgcgcctgaa tccgatccgg cccatcgagc tcaccgatca ccgccatgcc
      421 ggcgtcccga atgcgcacat gatcctgagg cgcgaggcgc aacagcgcgc tcaaatactg
      481 cgctccccat tgaaaccggg tcgcgatccc ctccgactcc cggtaggccc tttcgtacca
      541 cgcgagcgcc tgcgtcgtgt ggtggcgaga ttcctcgatt tcaccgaggt cgatcatgta
      601 gtagtagggc gtatgggagg tgctcagttc gccgctcacc agcgcgtact gggcatctaa
      661 gtcgccgagt tgctcataga cgtcggatgc cgcattgact acgcccgcgc gcacataagg
      721 atcgaatttc ttggcgagtg tcgcgttggt gcgaattcgc gccgcggcgg cgatggcggc
      781 tggcactttg ttatccgccg caaactcctt gagcaatgcg agccgcgtgc caagggctga
      841 cagttggtcg gcatcggtga cggcgggatc ggccgccacg ccctccatcg tgccgctcca
      901 ggcgtgcatg aaaacctcgc gtacctcagg accccgggcg tagaccgcct tgtagaacgg
      961 ggaaccgaga ttttccaacg agtcggcggc ccgtgccgcg atggagggat tctcgacgat
     1021 ggcgatcact tgcgtcacgt tcgacgctgt cggagcgaag accgccgagg cgatcgctat
     1081 tctcgctccc tcgagtgact tggcgccgcc gcacggcgtc actgcccgcg ccaaaccccg
     1141 cgccagcgac ttgcgctcga cctgcgaaaa gtcgcgcaaa tcccagccgt agtaggcaag
     1201 cctgcggcaa tcatcgctcg attcggccgt gccgctgcgc attgcagcaa tgacgtcgtc
     1261 catggggcgc aggtcattca acgcggcgtc aagcaacccc gcatacacgg acaagtccat
     1321 cccgccgctg atccgcgtga tctctttttg gtcgggcctc agaatgacca cggtggggta
     1381 gcccgatgcg ccgaatttgt cgccccactt ctgcgatccc tgggcgtcgc cgtcgagata
     1441 aacgtcgatg aactccctgg acttggcgat aaagtccttg cgcgagaaga ccgtggcttt
     1501 caactgctgg cacggcgggc accacttggc gccccaataa agaaataccg gctttttctc
     1561 ggtttgggcc aacttgaagg cctccgccac gtcaccctga aaccaagcga taccgggggc
     1621 gccgggcgcg gcggcgaccg gcacttcgcc gcgcgcgcct gccgccatgc agaacatcat
     1681 caaggcccgg gtggcgtatc ggatcatgtc ctcgcgccgc agctcaagtt ttgggttcga
     1741 aaatcgcgta ccacttcttg caaggctcgg taacttccca gacgccctcg aatcccgcgg
     1801 gtacgacgaa ggcatcgccg gcgcgcagct cgagcttttc acccgacgcc gattcgatgc
     1861 gcacgcggcc ttcgatcagc acgcagaatt catgctcggt gtagctcacg cgccatttac
     1921 cggcggtgct cgaccagatg ccgcagaaga attgttgact tggttcgtgg tactgattcg
     1981 acacgcgcgt gcgcggaatg ccggcgagta tcttctccgg cgaaggattt gccgactcgc
     2041 cggtctgcag ccggtcgatg accacgatct tagggtgcac gctcatgcgc tatcgtaggc
     2101 tggggttggc ggcggaaaca agtgcggccc ggagccgctg gtgctccggg ccgccacaac
     2161 ctgcatcacc ttgcacgaat cattcgtttt cgcccacctg agccaccgcg gcctcgctct
     2221 gcgcttgcag tgtcaggcga acccgacgct cgagtgcgta gccgtcgttg tcggcggcga
     2281 cgctctggct ttttccgtag gcgttgactt cgagcacttc ctccttgatc ccggcggcga
     2341 gaaacaggtc gcgcaccgca ttggcgcgcg cctccgacag cttcaagttg ctgtccacgg
     2401 tgccgcgcgg atctgcgtag ccgtcaaccc tgactttgag agcgggagac ttggcgacgg
     2461 cttcggcgag cacctgaatc tgatgggcga tttccggtgt gatctcggcg ctgcccgtgc
     2521 ggaacaggac gtcgccctgc agaccgtcga gcacatcgct gccatctccc ttggcggcgt
     2581 tctgcgcgct ttccacctgg tgcgacagat catcgagccg cgcgctcaag cttcgattgc
     2641 tctgggtcag cgcagtcttt tcgtccgcca acgtcgattt ttctgtctgc agctccagct
     2701 tgtcggtctg caacgcagca acggtcgcct cagctcttgc cgcatccccg gcgcgatgca
     2761 cccggtttcc gagccaggtg ccgagcccgg cgccgatgac cgcacccacc gggccggcgg
     2821 cgaatgcgcc aatcacgcct ccggtcacga atccgatcgg ctcctgcgga gtagcgggct
     2881 tgggctcgcc cgcgtttgcc gccacgctga ttgccaatag cccgatggtc cgaacggttc
     2941 tcgaaaatac ggtattcatt tgagctcctc actgcggttc gccaggaagc gacgaccgtc
     3001 gcctcgtgag agctattagg ggcttacatt cgggcgcctc accgtcaggt ttggggcaaa
     3061 tagtggcgaa gcggcgaaca attcgcgatc ggccgcggcg gcgcgcctta cgctttcagc
     3121 gagaacggtg tgaagttggt gtcggtatcg tagtaatcct cgttctcggc ttttttcaag
     3181 aacgctacca cacgataagt aatcggcgtc gcaacgaatt cccagccgct cttcaatgcg
     3241 tactgcgtga acgtaatggc gatgagatgc gcgagcggca tgcggccgta gaaggccacg
     3301 aaatagaaaa tcaccgagtc gaccagttcg ccgcacaggg ttgagccgac gatacgggtc
     3361 cacagccagc gtccccgcgt ccagatcttc atcttcgcga gcacgtagct gttgacgaaa
     3421 gtgccgcaaa gaaaggcgac gatcgaagcg gcgacgattc gcggtgtatt gccgaagacc
     3481 gcttcgaccg cagcctgggt gccgcgcgaa ctctcggacg gcggcaggtg aaccaccacc
     3541 gccgacatca acgaggcgaa tgcgagggcc gcgaaccccg cccacacgac gcgccgatcg
     3601 cgcccgtagc catacacctc ggtcaatatg tcaccaaaga tgtaggaaat cggaaaaaac
     3661 aacacgccgg cgagaaacgt gagagtgccg aaaacaggca cttgaatcga tgtttccttg
     3721 gccggaccga tcaagttcgc gcacaacagg atgcagacgt aggccgccat tacgaaatcg
     3781 tagtagcggt attgacgggt ttgcggcgct gtagtcactg cgcaagtgta gcaagcgcaa
     3841 gggaattgcg atgatatgta acaccgaggc ggcaactgtc gcggtttggc gaagagcatc
     3901 acaccaaggc cgcacgctgt gcgcaatcat acgaactcaa acccaacgca ggacctatgg
     3961 agaacggcag acccttcggc gcgacagaca gtccgctcgt cgatttcgat gccgtctgcc
     4021 agaaggtgcg tgcagccgtc gagccagcgc gcgcccacgc cgtctcgctg cacgacgaac
     4081 agggcgaagt gctgtggttg agcgaaagct ccatggggcc ggacgaacat aatgcggtgc
     4141 gcgaagccgt cgacaagttc tcgagccccg gttccccgcc gctgttggtt gcggatttag
     4201 gagattcgcg cgccgccttg ctgttgcgcg ccgtgaacgg ccggcgagcg atggtcgggg
     4261 cgatcatgat tgtcatggac gcacgcgccg tcaaacagga cgctcgcggc gccctcagga
     4321 tcatgacgcc taaattgcag agcgcactcg ccgatttcgc cgagatgcga ccgggtcctg
     4381 caccggcgcg gccggcaggc cctcggccgc tggcctcgtt aggatcccca gccggtctca
     4441 ccgccaaacg tccgcaagcg gcggtaagcc ccgaaatcga tcgcttgcat gccgcacttc
     4501 gccgcagtcc aatcatactg cacgtgcagc gcctggtccc cttgaccaag ggcagtcagc
     4561 tcaagcgttt tgaagtgctg ctgcgctcca aatccgaaga ggcgccgcac gcggctccgc
     4621 aagcaatgct gaaggccgcg gtcgagcatg gactcggttc gatgattgac cgtcgcgttc
     4681 tcaccgaact catcggtttc ctggtccatc atcccgaggt ctggaaatcc tcggcagtca
     4741 tgttttccgt gaatctgacc accaccgcgc tgcacgatga gcacttcatc aaattcgtcg
     4801 aactgtgttt ggcgaaatcc tcgctaccga aggggatgat cgcctttgag gtggacacgt
     4861 cgacggcggt caagctcgcc gccaagatga ccgaagtggc ggccgcgctg catcgactcg
     4921 gctgtccgtt ggttctcgat gacttccagc tcagcaccga atgctttgca ttgttgcgtc
     4981 tgcccggcgt gcgctatgtc aagctcgctc cggaaatcac gtcgaagatg cgcaccgata
     5041 agttggcgca ggcggccatc accgccgtgg tgcaaatggc ccgcgtactc ggcatgcaca
     5101 cagtggcgaa acgcaccgaa actgcagcgg aacaggaatg gttgactgcg ctcggcgtag
     5161 atttcgtgca gtcgaactcg atgtcgcccg cggtcgcgat cgaatcgctc gccaagacgt
     5221 cgaacccgcc gaaaccgccg cggataccca ccgtctagtt gtttcgcgaa agccgcgcgg
     5281 cgtctgcgct ccaaccgccg ccgagggcct tcaccaagta gaccgctgtc gtgaattgct
     5341 ggccatgcac ctgcacagcc tgccgctgat ttccaagcag cacctgttgc gccgtgatca
     5401 tttccaagta cgtgtcgacg ccgcccttgt atcgggtggt ggcaatgtcg aacgcctgtt
     5461 gcgcgttctt tacacccgaa tcggcctgcg tcgcggcacg gtcgagggat gccaagcccg
     5521 tgattccgtt ttctacctct tccatggcgg tgagcacggt ttgccggtaa ctcgccacgg
     5581 cggcgctata atcggcgtcg gcaatgcgca cattcgcccg ggtcttgccc gcgtcgaaca
     5641 gcgtttgtgt cgccactagg ccgagcgacc aaagccggct cggcgctgcg aatagcgtcg
     5701 ccaaagcatt gctctgccag cccgcattgg gacttccaaa acccgaaccc aactcgatgc
     5761 tcgggtagta agcggcccga gccacaccga tgcgcgcatt ggcggcggcc atcgcccgtt
     5821 ccgcggaggc gacgtcggga cggcgctgca atagatccgc gggcatccca ggcggaattg
     5881 acggcagcga tgcggtcgca accgccgcgg gcagcgcaaa attcggggcc ggcgtgccaa
     5941 ccagagtcgc gatcgcgtgc tcgtattgcg cgcgttgatt ctccagcagc tcaagttgcg
     6001 ttgcgctcga atcaaccagc gcccgttgct gagccaaatc gagaccggtg gcgaatccaa
     6061 gatcatgtcg cgacccgatg aacccgagcg catcgcgttc cagccccaag ctgttgcgca
     6121 ccacgtcgac ctcggcgtcg agttctcgca gcgcgaaata gtcggtcgcg agttgcgcga
     6181 tcaggatcaa acgggtgttt tcgaaatccg cgtcggcttg ctgcgcggag gcccgtgcgc
     6241 cttcgacttg gcggcgaatc cggccgaaca aatcagcctc gtaattcact gcaggtccga
     6301 gcgtaaagtc gttctgcacc gtggattgat tcggttccga ataggcggca agcggccggt
     6361 tcgccgatgt cttgccacgc gcggcgccag cgctaagatc caccgacgga tacaaagccg
     6421 acgctgcgac ggtcacctgg tcgcgcgctt gatcgagccg cgccgcggca actttgatgt
     6481 tctgattgga gttgagcgcg cgttccacca atggattcaa ggcatcatcg tggaacagcc
     6541 gccaccactc gcccttcaag gcgccgtcgg ccggcgccgc cgcgtgccac ggcgctggcg
     6601 gctgccagct gggcggggtg tccaccgaag gcttgtggta atcgggtccc actgcacagc
     6661 cgctggcggc aagcacggcg ctccaccagg ttgccaagcg gcgtaaagtc ctgcgcgatt
     6721 cgtcggccac gctactcgcc cttggctggc gccggcaacg tcacgacatc gccttccgcc
     6781 agcgaatcag ccggattcag cacgatgcga tcggcggcgt tgagtccgct cagaacctcg
     6841 acgtcggcgc caaaatccgt ccccaatttt accgcggcca gatgcactcg cccgccgctg
     6901 tccaccaatg cgacgcgcgt cccctcgggc cgaaacaaca gcacattggt gggaacgacc
     6961 agcgcgcgtg cgtctgcgtt gatgggtaag gttacctgca cgtacgaacc tgcgatcaac
     7021 gcacccgttg ggttgggtat ttgtatctcc acttgcatgg tccgcgtagc cgtgtcgatc
     7081 gcccgtgcgg tacgcgctac ggtgcctcga tactgctcag cggcgcgctc cgccaaggtc
     7141 accgtcaact catcgccgat tttgatttga cgggcataca tttgcggcac atagatgtat
     7201 aggcgcatcg gatcgacctg cgcgatgctg aatagcgcct gtccggtgcc gccgttaccg
     7261 gcgttgacga gatcgccgac atcgatattg cggcgcgtga ccacaccgtc aaacggtgcg
     7321 agaacgcgat tgaatccctg cagcttctgc aagcggccca cattggcatc cgccgcggca
     7381 agattggctg cggcttggtt gaaagcgctc agtcgttcat ccaaatcctg ctgcgtcacc
     7441 gcgtctttct gccgcaacgc ctgccagcga tcggcggtgc tcttcgctaa actggcgctg
     7501 gcgtcggctt gcgcccgaga agacacggcc tgcgacaatt cctgatctat ttccggtgcg
     7561 gcgatctcgg ccagcagatc gccttttttg acggaacttc cgatgtcctt gacccagcgc
     7621 accacgtagc cgttgctgcg cgcatagacc gtcgattcga tgactcccaa cagtgttcca
     7681 ggcaaggtca gcggctgagc atcgccgccc gtcttgggca agatggtggt tacgtactgc
     7741 tttgcgtgca gcgccgtgga gctctccagg gcgcttgcct ggaagctgcg catcaccagc
     7801 acgatcacgg caccgatcag cagcacaccg aggatcgccc cggccaccca cttggcacgt
     7861 ttcacgaccg aaaaggtctc ggcttcgggc atatcaagta cttccttcgg ctaacggcgg
     7921 caccgcggag cggcgctcgg ctttgcgtga ttcgagccgc tgatgaatgc cggcgaatat
     7981 caccggcacg aacagcaagg tcgaaacggt cgcgaacagc agtccgccga tcacggcgcg
     8041 gccgagcggc gcattttgct cggcaccctc gcccaaacca agcgccatgg gaaccatacc
     8101 aatgatcatg gccaacgccg tcatcatgac agggcgcatg cgagttgctc ccgaatcgag
     8161 tgcagccttc acgggatcga gcccttcgcc catgcgctgc cgcgcaaacg ccacgaccag
     8221 aatgctgttg gcggtcgcga cccccatggt catgatggca ccggtgagtg cgggcacgct
     8281 caaagtggtg ccggtcagaa atagcatcca gcagattccc gccaatgcgg caggcagcgc
     8341 agtgatgatg atgaaggcgt cgacccagga ttgaaagttg acgacgatca acaagtacac
     8401 catgacgacc gccatcacca gtcccacacc caactcgacg aacgaggact tcatggtttc
     8461 cgtttggcca cgcatctcga cactcacacc gcgcggcagg tgcggccgca gttcatcgag
     8521 cagccggcgg atatcgaccg atattccacc catatcgcgg ccctgtgcac tcgaatagat
     8581 gtctatcgtg ggggccaggt tgtagcgcga atcgaccgcc tcctgcactg caggagtgac
     8641 gccgaccaga ttgctcaata gctgcggcgt tgtgctgagg gcggccgcgg ccgcgcgcac
     8701 cggtgtacgc agcacggcat cgagactgtc gaccgcatac tgcggcgact gcaccgccag
     8761 gctgtacacg attccattga ccggactcag ccaaaaggcg ggcgaggttt gcgtggtgcc
     8821 ggccgtcgaa accaagaggt cagtcgtcac gtcgctcgga gtcagaccca gctgctgcag
     8881 ctgtgtgcga tccatgttga gcgacacggt cggctgatcg agccgctgat gtatgtgcgt
     8941 atccaccgtg cccggaatca tttcgatctg tttcatgagc tttgcggcga taccatagtc
     9001 ggcgcgcaga tcggcgccgg tgaccttgac gtcgatcgcg gacggtagcc cgaaattgag
     9061 aatctgcgtc accatgtcgg cgggttggaa aaagaactcc actccgggga agcgctgcgg
     9121 caattcgcgc cgcatcgcct cgacatagcg ctccgtcggc cgatgtccga cattgagcga
     9181 cacttgaatc tcgccgtcgt ggctgccgaa agttccagcg ttgctatagg acttgttgat
     9241 tccgctcact gccacgccga gattatcgag tatggtacca agatccttgg gcggaatgat
     9301 cgaacgaatg acccgctcca cggcatcggc cacgcgcgcc gtctcttcga tgcgcgcacc
     9361 cgtaggcagc cgcatgtgca agcggatctg gccgccgtcc acgctcggaa agaagtcgcg
     9421 gccgagaaac ggtgcgagac agcaagacaa cacgcagaag ccgagaaatg caccggcgaa
     9481 tacgcgccgc cgttgcagaa cctgcgaaag gatgatcacg taaccgctgc gaattctctc
     9541 gaagcggcca ttgaatgccc ggtatacgcg ctgcagcacg ctcgctccgc tctgccgcgc
     9601 ggtgtgatcc atcaacagca tcacgagggt gggcaccagg gtgcgcgaca gcacatatga
     9661 ggccagcatc gcaaacacca cggcttgcgc cagcggggcg aacaggtagc gcgctacgcc
     9721 ggtgagaaag aacatcggta cgaacacgat gcaaatgcac aaagtggaca ccaaggcggg
     9781 aacggcaatc tcgcccgcac cttccaaaat ggccttgtgc aggtccgacc ccaggtgcat
     9841 gtggcgctcg atgttttcga tcgtgacggt ggcgtcgtcg accaaaatac cgaccgccag
     9901 cgccaagccg cccagggtca tgatgttcat cgtctcaccc atggcataca gcgcgatgat
     9961 cgaggacagt atcgacagtg gaatcgacac ggcgatgatg caggtgctgc gccaattgcc
    10021 gagaaacagc agtatcagcg cggccgtgag gcaggcggcg atcaaagctt cgcgcaccac
    10081 gccgctgatt gcggcgcgca cgaaccccga ttgatcgaat agcggcgtaa tcttcagttc
    10141 cttcggcaga atctgcgcga ctatcggcag ttgcgcgagc agcgagctga cgatgtcgag
    10201 cgtcgatgag ccgccattct tgatgacgga caccaataca ccgcgctcgc cgttctgccg
    10261 cacgatattg gtctgcggtg aaaacccgtc gcgcacgtgc gcgacctccg acaagtaggt
    10321 cgtggcaccg ttgcgcgtca acacgggaat gttgttgagc ccggcgatgg tatcgggcga
    10381 tccgttggtc gcgagcacgt actcggttgc gccgatcttg gcggtgccgg acggcaacac
    10441 caagttctgc gtgttcacgg cgttcaccac gtcgttgggc gtcaggccct tggcgagcaa
    10501 ggcatcggtg tcgaggtcga ccgaaatcac gcgctgcttt ccgccgtagg gataggggat
    10561 ggctaccccc ggaatggtca ccaggtgcgg acgcaggaaa ttcaccgcgg tatcgaatac
    10621 cgcctgttcg gtcatgctgg ggctggacac cccgagctgg atgatcggaa tgctcgatgc
    10681 cgagtacttg atcaccagcg gcggcaatat gccgggcggt aattgcttca gctgcgactg
    10741 ttctattgcc accacctggg cgatcgcggt cggaatgttg gcgttcggct gcaggaaaat
    10801 cttgatgact gcgaagccgg ccaaggactc ggactcgata tgctcgatgt cgttgacggt
    10861 tgtcgtgagg ctgcgctcat tgaccgatgt gatgcgctgg ccgatctcct gcgccgatag
    10921 tcccgtgtag gtccatacga tgctgataac cgggatattg atttccggga agatgtcggt
    10981 cggcattttc aacagcacca gaggggtcgc tagaagaatt agcagcgcca tcaccaggaa
    11041 cgtatacggc cgccgcagag caacttcgac aatccacatt tgcttatcaa ccttgcgttc
    11101 gagcgcacca gcgccatttc accgcgtaac cctgggctcg taggagttaa caccctgtgg
    11161 tcgatttatt aaagtctgtt aatgcgcgag taaggtgtgc gcggcgccgg ctgcgagtgt
    11221 gacgcgggtc accagcctga cttcatatcg cgacactact tgcaaatcat tggaaacaaa
    11281 gcaaactatg cccgtctgca acgagacacc gggaccgagc taagtgtttt tgttagtccc
    11341 atgaggttcg gttccggttc ccgttaggtt tccccatgac actcattgcc ctgatcatcg
    11401 tcgcgctggc gggaatccta ttcttgttcg cggccatgcg ccgcctgcgg caacgcaagg
    11461 tcatcagcgg tctgtttcac ggcgcaacgg cccttgtgct catcttggtg ggcgtcggcg
    11521 cagcgctgct gttgaccaat ctgcgcacct atcaacgcct gagtttcgag caacgggccg
    11581 gcgagctgca attgacgcgc accggcgatc gtcaattcaa tgctgtcttg agctatccga
    11641 acggcgagcg cgccaatttc gcgttgcgcg gcgatgaatg gcaggtcgat gctcgcgtgc
    11701 tcaaatggca cgcctttgcc aacctggttg gattcgatac ggcgtatcgc ctggagcgtc
    11761 tcagcggtcg ctactcgcgc gtcgaggatg aagtcgctca accgcgcacc gccttttcgc
    11821 tgaatccgcc gcaacacatc gatccctggg tcctggtgca tcgctatcga tcctgggtac
    11881 cgtggatcga tgcggtgtac gggagtgccg ccttcctgcc gatggcggac ggtgcgctct
    11941 atgagatcag cgtgtcgcag agcggcttga ttgcgcggcc gctcaaccag gctgcgcgcg
    12001 acgctgtcgg cggctggcac tgatggtgcg acgtgtcgcg cggttcgcgc tgatcgcggg
    12061 cgctgcggtt gtgctgctga tggcgctcgc catggcctgg ctcaaaattc atgagactga
    12121 gttggttttc aggggtgcga ggagccgcga gcatctcctg aaagcgctgc cggcggaggc
    12181 ggaacgggtc gccgtggacg aacccggcgg cattaagctc gcgggcacgg tgtttcatgc
    12241 cgccgctgcg gatacggcgt tctgggtatt gcacctgcat ggcaacgccg attcggcctt
    12301 ctcgccatgg caggtgcgcc attgcaaggc actgcgcgac gccggtttca atgtcttgga
    12361 catcgactat cgcggcttcg gggagacgcc gggcgagccc tccgaagccg ccatgtacga
    12421 ggactcggag gcggcatacc aagaactcct gcggcgcggg gtgccgcaac ggcgaatcat
    12481 cgtgctcggt cactcgttgg gctcgggccc ggccgtgttc ctcgccacgc ggcatcaagc
    12541 agcggcgctg gtactgttcg gcgcgttcac atcgattccc gacgcggcgg ccggccaata
    12601 cccctacttg ccggtgcgac tcgccgtcgg ggtgcagttc aactccttcg cccgcatcgg
    12661 tgaggtccat ataccggtcg tcattaccca cagccgcggc gacacgctga tcccctattc
    12721 gcacgctctc aagctgctgg cggctgcgaa tgagcccaag cgtctgttga ccttcgattc
    12781 cgcaaccgac ggatttggcg gccatgtcga cgcactgtat gagcatgttg atatacttcg
    12841 ggcgactctg gcgagtctca taccgtccat gcagccgcct ccacaataat gacagtcgca
    12901 caaaagcttt ccactctgct gctgacgctg ctgctggcgc tcgcgggctt tgcgtggtgg
    12961 aagaccgcgc cgcccacggc caaggtgacg cgacagcggg ccgcggcact ggccgcagcc
    13021 gaactggtcg atcaaagcac ctacaacaca gcgcaacgac tagcccagct cgccgatacc
    13081 ccggaagagc aagcctatgc ccaagcggcg ttgcgggtcg cggatcatgc tctggccttc
    13141 gcattcacga cggcgctgcg cgacgtcgaa gcgcatccgc cgcctttgag tcccgaagcg
    13201 ctgcaaatcc aggatcgaat tcgcaagtcg caagtattgc tcgacaccga tcagcaacgg
    13261 gtgaccgaac tggccggcac gctcgctcag gcgaaggccg cgcagaagga tgccgtgcag
    13321 gacgagttgg atctcgcgca gtcgcaactc gacctggata aggatgaact cgaagaggcc
    13381 aatcaagacc tgatcgaggc cggcggcaac cagcaaaagc gcatcgaagc tttgcagcaa
    13441 gagcacgaag cgcaggtgaa agcgcgcgcc gcccctccgg ccgccggcgc tgctagcgct
    13501 ggcacagccg ctttcgagaa gcacggtgcc ttcggccgac tgatcgagtg gcgccacctc
    13561 tggctgaaac aacaggagct gttcgatgcg cagtcgagcg ccgcgcagaa ggcggcgaat
    13621 ttgacggggc gacgcactgc gctcgcggcc gcgctcgaaa aagccaagag cggcatcacg
    13681 gaactcgcgc aacacaccaa gagcgcgaag caagagggca cggctgctgc gccccaggta
    13741 cgcatccagc atacgcacga ggacgcggcc gcgctgctga atcagacgaa gttgatcgca
    13801 gcggaccaga aggtgctgac cttgctcgat cgccgcaccg ccgatcagaa gcagctggat
    13861 tctatttacg gtcagtggtc cgcgctggtc acgctgcaag cgcatacctt ggcgcactct
    13921 ctgctgctga gcgccttgat cgtcatcgcc atactgatct tggtgatgct cctcgacggc
    13981 tggacgcagc gtctgctcga caatccgaag ttggatcggc gccaggtcga aactttgcgc
    14041 agcatcgcac gcgtcactct gagagtcatc gccgtggtca tcatcttatt gatcgtcatc
    14101 ggcctgccga cgcaattcgg caccatgatc ggcatcgtcg gcgcgggcct cacggtcgcg
    14161 ctcaaggact tcatcgtcgc cttcttcgga tggttgatgc tgatgggccg caacggcatc
    14221 cgtctcggcg actgggtgga aatcaacggc gtcagcggcg aggtcgcgga actcggtatg
    14281 tttcatacgg tattgatgga gaccggcaat tggagcgatg ccggacatcc caccggccgg
    14341 cgagtgacct tcaccaacag cttcgccatc gaagggcact atttcaattt ctcgacgtcc
    14401 ggacagtggc tgtgggatga gttgcaggtg gtcgtgcccg ccggccgcga tccctacccg
    14461 atcgtcgacg ccatcaccaa gcaggtggtc gaggcgacgg cggagagcgc gcggcaagcc
    14521 gaacaggaat ggcaaagggc ggtcccggcg cagcgcggca aggtcttctc aggcacgccc
    14581 ggcgtcaacg tcaaaccggt tgtcggcgga atagaaatcg caatccgtta catcacgcgc
    14641 gccaacgagc gctttctact acgcgccaaa ctgtaccaag cggcggtcga cctcttggga
    14701 gacaaatatt cggcgcagaa gccgaccacg gtataatgcc tgccaccgac gccggcttag
    14761 cacagtggta gtgcaacggt tttgtaaacc gtaggtcggg ggttcgaatc cctcagccgg
    14821 caccacctac gggttccaga acgtcccaag agatttctcc gcgcaagaaa acgcctaaga
    14881 tttacggcat ttttgttcct gcaatgtctc aacgcgtccc ttgaaatccc gtcgtcatgg
    14941 ggcatatttg ggggcatagt ccgttcacga aaggttgatg cccccaagtg cttaccgata
    15001 ccgcgatacg tgccgccaaa ccgcttcaga aggcgtacaa gctgtttgat agccaaggtc
    15061 tataccttga ggttgccccg accgggagca gactgtggca tgtcaagtat cgttacgacg
    15121 gtagagaggt agagaaaacc gccttgcttt gggcgcatat ccttccacct cattgaaaca
    15181 ggctcgtgag cggcgtgacg cggcgcggac gctgctgggc gatggcatca atccaagcag
    15241 taagcgtcaa gccgagaaat tggcaaatgc gaatacattt gaggcggtcg cacgagaatg
    15301 gctgttgcta caacaaagca aactggcaga gtccacctac gcgaaggccg tctggacctt
    15361 cgaaaccctt gtgttcccat atattggctc acgtcctatc gccaagctgg gtcgtcccga
    15421 actctggggt cagtacgggg attgtttctc tgtacaggtg aatcgagttg tgactttggc
    15481 tcagtgggtt tttgcgttct acacctcacc cgtgtttcga ctcgaacgag tcatcttgca
    15541 tgtcttggcc cgagcgccat cgaacgatca gcaggcacag gatgtcgcgc aaggccggcg
    15601 cgagacgttt gcggtgtggg tggtgggcgc acgtacgacg gatcagctat taatgtgcga
    15661 tcggtatggc aagacgcgct catggttccg ggtaacaccc caggagtcgg gcggaacggt
    15721 tctgcagttc ggctcagcgg tggccgcgcg ccgtggctcg cagggtggga cccatatgag
    15781 cggtgggttc agtgtcttgc tagggctcca caaaatctat tcaaaactgt tgatcggcgc
    15841 ggcgaagcat gcgctatgtc agggcataac ctaacgccct gatcaggacc ccgagtgggc
    15901 ggtccagcgt aactaccggc gtggtcagtc acagcgtgat ccgccgaaca gctgccgttt
    15961 gtggatggct cctcagggtc cgactgcggt cggattgcgc ggcgccccaa acaatcccca
    16021 tcctgatcgg gaaggtcgtg atcggtaacg actcatcgcg atggcttgcc gcgacattat
    16081 tgcggtagct acataaatgt agtaacattt gtgtatgatg acgcggtttg aatgggaccc
    16141 cgccaacgct ggggatcgtg gacgggtatt tactcctcgt ggtggcccac accatccggg
    16201 atgaagagga ggacggacag ccgattgaga tcatccgaat tatctcggcc cgagcggccg
    16261 accggaaaga aaggcgacgt tatgagcaag aaattcgtta aacgcgcggt cgacctagcg
    16321 aacctgcctt ccctgactaa aaggcagaaa gcggatctcg cggcattggc tgctcggccg
    16381 gatggcgaga tcgacttcag cgacattcct cctctgaccg aggacttctg gaagaacgcg
    16441 gcgcgcggca agtattacaa gccgaccaag acctccacca ctgtgcgaat cgattccgat
    16501 gttctagctt ggctccggtc tcagggcaag ggctaccagt cccgcatcaa cgccatcctg
    16561 cggcgtaaga tgctggagtc gctcaaataa ggaggagcgt gcgatgccaa tcgatataga
    16621 cgcgttgacg gaacccgaac tcatcgatct caaccaccga atcgtggcgc ggctgcgatt
    16681 cctgcggcaa attgacgcac acgcaaccat gctcgagttc cgaatcggcg agcgggtgac
    16741 tttccgtccc gacgggcggc caccggtgac gggaatgatc acgcgctaca acaagaagac
    16801 ggtgagcatc gtcaccaacg atggtcagcg ctggaatgtg gctccgcatt tcctcgagtg
    16861 cgcagcgcct cgcgcgacta gcaagcctgg cggcgataac gtgatttcaa ttcaacggaa
    16921 cgacaagcat tccaataaat aacggcgccg cggggtaact tcaggggtaa ctcattttat
    16981 tgagattcca tgactcaatg gaatcatagt gatagcgcga ttattcgaat ccctcagccg
    17041 catttttacc cacggatatt tttggggcac cgcctttgct gcgctagtta actggcgtcg
    17101 aacacgacca ccgcgtttcc cgactcgatg ggcgaaaaca aggcgtcatg cttgtacgaa
    17161 atagacagga tattcataaa ggcgggagcg taagcgttgc ggtcttgccg ttgctggata
    17221 tgtaacggtc tagcgaagga tcgagttcca ctgcagtcgt gacgtcgctg acagcattag
    17281 gcgagtcgcc gaggtctccg aaggctgcgc cacggagaaa ataagcccga gccatcttcg
    17341 gatcaagccg gatcgcttgg gcctcgtctt tgatggcatg ttgagtctgg tggattctcg
    17401 cgcgctggag cagcgcgggg accagtttag gattgaggct caaggcggtg ccgagatccc
    17461 gcaaggccgc atcgacatca gtttgcgttg cgtagaaaac cccgcggtag tagtaaggtt
    17521 gcgcgtattt gggagccaat ttgacccctg tattgtaatc gtcgagcgca tgctgcttat
    17581 cgccctgggc aacataggcc acggcacgat tcacaaacaa atagaagcgc tcatggccct
    17641 gaagtatatt tcggtccagc aactcggtgc atgcctgaat gatcacgggc ggcgcgctct
    17701 tcccgtcgcg attgatgcac ttgttgatga cgttacgctg gtcaaccgcg agctgggcct
    17761 cacaggtctc catggccacg aaatccattt gttctggatc tgtcgattta gacatcgcat
    17821 aacagttcgt gaattgatcc ggtgcaagct ttggcggcgg cggctcggca cgcggcgtgg
    17881 tgccagtgac cgtaattccc gggctaatcg ttggctgtgt tgtcgttgcc tgaccgtcgc
    17941 cgggcggtgt tgcattctct gcgggtgggc tgtctggtga ctggccgccc tggtttgaat
    18001 tgttgacctg agcgcccgcc gttattccgg cgaagagcaa aactgagagt tgccacgaga
    18061 ataaacgctg cacgatttcc atccgagaac gaactagttc ggcacgccgc cgccagagtt
    18121 aagacccgtc aaaagctcag cgagttccat ctggcatccc gagtaaacat ccgctggtcc
    18181 cgccaatgat cgacgccggg gagagtggat cgcccgagaa tctgatgagc gaggctatcg
    18241 ccgccgttct cgctgtcaac ggcggcgcgc gaagcgcatg cacccctgac cggggcgcac
    18301 ccggccatcg gtagaaattc accggagcaa attgctagca catagcggac gttcaaaagg
    18361 ctgtcctgcg cgaaaatatg cctcagcggc ggacctagca taaaccgcca tcagcattgg
    18421 ctgcgctcgg ccgtacatca gtgatcgcca aaaccattca agaggtccga atcggaaacg
    18481 gcgcaaccat gccgcactta gaaccgcttg cagtaaataa acgataacgc cgaacagcaa
    18541 ggcgggcgca gcattcatct ttccaaatag accgaggcca tagccgaaga agacaaagcc
    18601 gaagatgagc gactgcgcaa tgtaattggt gaatgccatg cggccgacag cagcaaacgg
    18661 ccggagtacg ttgtgcgcgc catgcaactg cgcgaggtag atcacaacag acccgtaccc
    18721 gactgccaac acaaccgggg cgagcgcctg taggaccatg ccgagcgtgc cccagctgtt
    18781 gaacgctccc gccgtggcgc cgtaggttaa tgccatcccg actcctaacc ctgctgccgc
    18841 aaccacagag aacagtctcg agtgactgga tggctgccga agtactccca ttctccaaac
    18901 gaacgcaccg aacaagaaca gagcgaccgt tctagggaat acaaacacgt gtagtggcac
    18961 aaggagcggg agttcgtgca cgctaaagcg ccagccctcg aggtagaaac ccgcagagta
    19021 gacgcgatcc gcagtcgcga cgtcactagc aaacgccgaa gtatccggcc atgcgactag
    19081 cggcggcaaa ctcggcatca gtgcataaag tgtgaagaat cctgccgctg caacggcaag
    19141 cgcccaattt ggaagccgca gaaacggcaa tatcaacagt ccggcaagag cgtattccgt
    19201 gaggatgtcg ccgttccaga tgaatattag atggatcagc ccaaatccga gcaatgcgac
    19261 cagtcgccgg gtcaacaaaa ggtacggggc ggggcgtcta gagagccgtt ctaactgcat
    19321 cgccagcccg acgccaaaca ggaacgagaa tacgcagaat gccttagact cgagcgcgaa
    19381 cgacacgaaa tcctcgacga atccgttgac tcctgtcgct ggtgtgctgc cagcaataaa
    19441 ctgctggaaa attgatactc gaaactccat tacaaggttg acggctaata caccaaacaa
    19501 cgcaatgcct cgtaacgcat cgatagagtc gatgcgatca cgtggcggag tagccgttgt
    19561 gacaaaaccc ggattggtac ttttggatag cgcaggcata tcgcatcttc ccacatgccg
    19621 ggttacgaac gcgacagccg caaagctgac gtccacgacc ggcgggggct gagaatactc
    19681 attggaggtc ggtggacgcg ccggagtttc cagtcggccc gtagccgccg atgcgggagc
    19741 tgatattcca tcgccgaagc ggccactcgc tgcctgcaat cagctagagt attgacgatc
    19801 cggcgccgtc gccaaatggg gagataccct tgcgcttgcc cgctcttctt ctgtttgccg
    19861 cattgcattc cgcccgggca ctggccttgc agcccgttcc tact
//
LOCUS       DASWZT010000456        10254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_42524, whole genome shotgun sequence.
ACCESSION   DASWZT010000456 DASWZT010000000
VERSION     DASWZT010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10254
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42524"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VGE92_13005"
     CDS             <1..822
                     /locus_tag="VGE92_13005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY1314792.1"
                     /translation="KVDRVARKPRDIVAAGSRVRVQMRVAEVAQPEVLPEAIALSLIH
                     QDSDLLVIDKPAGLVVHPGAGNPRHTLQNALLGLDPDLAALPRAGLIHRLDKDTSGLL
                     VVARTPQAQTSLSRQLEARTMSREYVAACVGVMTGGGTVDEPIGRHRGDRLRMSVRAA
                     GRRAVTHYRVVERFRGHTYVSVKLETGRTHQIRLHLSHIKYPIVGDPVYGGRLALPRG
                     ATPGLIHALRSFKRQALHAATLGFVHPSSGKPMRLQSPLPADFEGLLGALREDAR"
     gene            819..1562
                     /gene="pgeF"
                     /locus_tag="VGE92_13010"
     CDS             819..1562
                     /gene="pgeF"
                     /locus_tag="VGE92_13010"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEY1314793.1"
                     /translation="MSPAWMLPTWPAPAGVRVISTYRGGGSSVAPYASLNLGAHVGDV
                     PAAVADNRRRLAAAAGLPAEPMWLTQVHGANVVDLDAVDASGPAGAADACFSCRRGRV
                     CAILTADCLPIVLTRDEGQLVAAAHAGWRGLAAGVIEALVKALAAPPASLLAWLGPAI
                     GPGHFEVGAEVREALVRGDAGANAAFAVNPRGRFMADLGMLARRRLAALGVDRVYGGA
                     ECTYADGERYFSHRRDGTTGRQATLIWRE"
     gene            1608..4193
                     /gene="clpB"
                     /locus_tag="VGE92_13015"
     CDS             1608..4193
                     /gene="clpB"
                     /locus_tag="VGE92_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEY1314794.1"
                     /translation="MRMDKLTTKFQAALADAQSLAVGRDNQYIEPVHLMAALLDQQGG
                     TARPLLEKAGAQVAKLRSDLAGALDRIAKVEGSGGEVLLGNELNKLLNVTDKLAQQRG
                     DQFVSSELFVLAACDDRGELGRLLKANGATRAAIEKAIDEVRGGAKVEDAGAEENRQA
                     LEKYSIDLTERATSGKLDPVIGRDDEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPEGLRNKRILSLDLGALIAGAKFRGEFEERLKAVLNDLAKQEGQVILF
                     IDELHTMVGAGKAEGAMDAGNMLKPALARGELHCVGATTLDEYRKYIEKDAALERRFQ
                     KVLVDEPSVEDTIAILRGLKERYEVHHGVEITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAGSLIRMEIDSKPEALDKLDRRIIQLKIEREALKKATDEASRKRLSTLEDALKA
                     LEKEYSDLEEVWKAEKASVQGAAHIKEELERARQDVDTARRAQDLARMSELQYGRIPA
                     LEQRLAKALEVEQKPNQLLRNSVTEVEVAEIVSKWTGIPVSRMLEGERDKLLRMEEVL
                     GRRVVGQGEAVTAVADAIRRTRAGLSDPHRPSGSFMFLGPTGVGKTELCKALAEFMFD
                     TEDALVRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRRPYCVILLDEIEKA
                     HPDVFNVLLQVLDDGRLTDGQGRTVDFRNCVVIMTSNLGSQVIQELAGEANYAKMKQA
                     VMQVVGQHFRPEFINRVDEIVVFHSLGREQIRSIVDIQLGRLRQRLAERDITLRLDDA
                     ARDKIGEAGFDTVYGARPLKRAIQAQIENPLAQKILSGDFVAGDRVIAKLKKDALVFE
                     KERLH"
     gene            4266..4736
                     /locus_tag="VGE92_13020"
     CDS             4266..4736
                     /locus_tag="VGE92_13020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEY1314795.1"
                     /translation="MPFYEYQCKNCGHDLEAMQKISEPPLKKCPHCGKPQLQRLMSAP
                     VFRLKGAGWYETDFKGDKDNQRNLADRPEADLPAEVAKDLAKDTAKEGTADAKAGANG
                     KDVKDAKEAKAEKGADKPVEKAAKEPANKPQARRSKTRAKKPLRKPAPPARRRG"
     gene            4738..6510
                     /gene="aspS"
                     /locus_tag="VGE92_13025"
     CDS             4738..6510
                     /gene="aspS"
                     /locus_tag="VGE92_13025"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEY1314796.1"
                     /translation="MRTHYCGQVNESLTGQVVSVAGWAQRRRDHGGVIFVDLRDREGL
                     LQVVFDPDKAEMFASAERVRNEFVLKVTGLVRARPPGTANANLKSGQVELLCQELTIL
                     NRCEPLPFQLDEHVSEEVRLRYRYIDLRREEMRERLLLRHRITRAMRHYLDEAGFIDI
                     ETPMLSKATPEGARDYLVPSRTHAGKFFALPQSPQIYKQLLMIAGFDRYYQIVRCFRD
                     EDLRADRQPEFTQLDIETSFLDQPDIQKLMEGLVRYLFKQVLAVDLPDPIPRMSYAEA
                     MRRYGSDKPDLRIPLELVDVADLVKGCDFKVFAGPAADPDGRVTALRVPQGARLSRKE
                     IDDYTAYVARYGAKGLAYVKVNEVAKQREGLQSPILKFLSDAAVGGILERTGAADGDL
                     IFFGADSRKVVSDALGALRLKVGQDLKLGATGWRPLWVVDFPMFEWDAQTKRWQAMHH
                     PFTSPADLDYAALAASPGEATAKAYDLVLNGSEIGGGSVRIHSQELQSVVFDLLGISP
                     EEARLKFGFLLDALKFGAPPHGGIAFGLDRLAMLMAGADSIRDVIAFPKTQTAADLLM
                     DAPTEVSEAQLRELHIRVRVPPTP"
     gene            6511..6957
                     /gene="nudB"
                     /locus_tag="VGE92_13030"
     CDS             6511..6957
                     /gene="nudB"
                     /locus_tag="VGE92_13030"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: protein motif:HMM:NF006961.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEY1314797.1"
                     /translation="MNAGTFRRPESVLIVIYTEGGEFLLLERRRPPGFWQSVTGSLEW
                     GEFADAAARREVAEETGITQGVLVNLQWTQVYDILASFGKIYAPGITRNLEHAFSLRL
                     AGRVPVTLSDSEHVGYRWVSGEEAVETVSSSTNRAVIEDLRLGVAP"
     gene            6954..7595
                     /locus_tag="VGE92_13035"
     CDS             6954..7595
                     /locus_tag="VGE92_13035"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY1314798.1"
                     /translation="MSTVIVYVHGLWQRGAESLWLRRRLAQDLDADARIFSYPSVAAD
                     ATANSRALAEYLSAIGADTLHLIGHSLGGLVILKLFEEMAQARAHWPPGRIVLLGPPL
                     RGSRTAQNLARIPFGKRIMGLGVSEELLVPRERTWSGSRDLGVVVGDLGVGLGRLAGS
                     LGGPNHGTVLVEETQLPGATDRVVLRVSHTGMLFSAAVARQACVFLRTGRFAH"
     gene            complement(7609..>10254)
                     /gene="mutS"
                     /locus_tag="VGE92_13040"
     CDS             complement(7609..>10254)
                     /gene="mutS"
                     /locus_tag="VGE92_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005251491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEY1314799.1"
                     /translation="LYSTNYMKYQKETDFAGPGSQHPDHTPMMQQYLRIKAQHPDVLL
                     FYRMGDFYEMFYDDARRAAQLLDIALTQRGASAGAPIPMAGVPAVTLDAYLARLVRKG
                     ESVAICEQRGEPGKTKGPMEREVVRIVTPGTITDEALLDERRDNLLASVFGANGRYGL
                     AWLDLAAGRFTIMELPGAAALEAEIERLRPAELLAPEGAERLSERPFRLRPPWHFDTE
                     SATRALTEQFRTRDLAGFGCADKPLAIAAAGALLAYVRDTQKSALPHLLSIAVEERDA
                     ALIMDPATRRNLELDESLAGNAELTLAGVFDRTATAMGGRMLRRWLHRPLRDNGVLRA
                     RYHAVATLMEGALHTAVAQPLEAIGDLERIVARIALRSARPRDLAQLRAALGVLPQLQ
                     AAVGSAASPLLQQLIVELKEHNDDRAQDHALLSRALVDSPPHHLRDGGVIAPGFDAEL
                     DELRLLGTNTEQFLLDLEHRERERTGLSSLKLGFNRVQGFYIELSRSQAAAVPAEYLR
                     RQTVKSAERFITPELKSFEDKVLGARDRALAREKALYEGLLDHLTSRLAALQASTAAI
                     AQIDVLACFAERAAALDCAQPELVDEPMLLIEAGRHPVVERAGREPFVPNDLRCDDSR
                     RMLIITGPNMGGKSTYMRQTALIVILARIGCYVPARRVVLGPMDRIFTRIGASDDLAG
                     GRSTFMLEMTETANILNNATAQSLVLMDEVGRGTSTFDGLSLAWACAAFIANKIRAFT
                     LFATHYFELTSLAGEAPGVVNVHVEAVEHGDTLVFLHSVKDGPANQSYGLQVAALAGI
                     PKSVTAQARRYLSELERERDALRTSTSPQTELPLFAPASAPAPESAALEALRALDPNA
                     LSPREALDLLFRLQTLDRGGRGEHG"
BASE COUNT         1873 a         3030 c         3514 g         1837 t
ORIGIN      
        1 aaagtggatc gcgtggcccg caaaccccgg gatatcgtcg cggcgggctc gcgcgtccgc
       61 gtgcaaatgc gggtcgctga agtggcgcag cccgaggtgc tgcccgaggc gattgcactg
      121 tccctgatac accaggattc cgacctgttg gtgatcgaca agccggccgg gctggtagtg
      181 catccgggag cggggaatcc gcggcatacg ttgcaaaacg ccttgctcgg gctggatccg
      241 gatttggcgg cgctgccgcg ggcaggcttg atccatcgcc tggacaaaga taccagcggc
      301 ctcttggtgg tggcacgcac gccgcaggcg caaacctcac tcagccggca gctcgaagcg
      361 cgaaccatgt cgcgcgagta tgttgcggcc tgcgtcggtg tcatgaccgg cggcggcacg
      421 gtcgatgagc ccattgggcg gcatcgcggc gaccggctgc gcatgtcggt ccgcgcggcg
      481 gggcggcggg ctgtgacgca ttatcgggta gtggagcgct tccgtggtca cacctatgtg
      541 agcgtcaagc tggagaccgg ccgcacgcat caaattcgcc tgcatctgtc gcatatcaag
      601 tatccgatcg tcggcgatcc tgtgtacggc gggcgcctcg ctctaccgcg cggtgcgacg
      661 cccggcctga ttcatgcgct gcgcagtttc aaacgccagg ccttgcatgc cgcgacgctg
      721 ggattcgttc atccgagtag cggcaagccc atgagattgc aaagtccgct gccagccgat
      781 ttcgaaggtc tattgggtgc gttacgcgag gatgcccgat gagtccagcc tggatgctgc
      841 ccacatggcc cgctcctgcc ggggtgcggg tgatctcaac ttatcgcggc ggcgggtcga
      901 gcgtggcacc gtacgcgtcg ctcaacttgg gcgcccatgt gggcgatgtg cccgcggctg
      961 tagccgacaa tcggcgccgc ttggcggcgg cggccggttt gccggcggag ccaatgtggc
     1021 tcacgcaggt acacggagcg aatgtcgtcg acctcgatgc cgtcgatgcc tcgggcccag
     1081 ccggcgcggc cgatgcctgt ttttcgtgcc ggcgcggccg cgtgtgtgcg attctcacgg
     1141 ctgactgctt acccatcgtg ctgaccagag atgaaggcca gttggtggcg gcggcgcatg
     1201 ctggctggcg cggcctcgcc gcgggggtaa tcgaagcgct ggtgaaggcg ttggcggcgc
     1261 ctccggcctc gcttctggcg tggctgggtc cggcgatcgg gcccgggcac ttcgaggtgg
     1321 gggcggaggt tcgcgaggcg ctggtacgcg gtgatgcggg agcgaacgcg gcatttgccg
     1381 tcaatccacg cggccggttc atggcggatt tgggtatgct cgcgcgccgg cgcctggccg
     1441 cgctcggggt cgatcgcgtc tacggcggcg ctgagtgcac ctacgcggac ggggagcggt
     1501 atttctcgca tcggcgcgac ggcacaaccg gccgccaggc gacattgatt tggcgggagt
     1561 gacccccact tagtaaagat tcagagcgtt atctcaaggg atttgctatg cgaatggaca
     1621 agctgacgac taaatttcaa gcggccttgg ccgatgccca atcgcttgcc gtgggccgtg
     1681 ataaccaata catcgagcct gtgcacctga tggccgcgct gctcgatcaa cagggcggta
     1741 ccgcgcgccc gctgctcgag aaagccggcg ctcaagtcgc caagctgcgc tcggacctgg
     1801 ccggggcgct cgaccgcatc gccaaggtcg agggatccgg cggcgaggtg ctgctcggca
     1861 atgagctcaa caagctgctg aatgtcacag acaagctcgc ccaacaacgc ggcgaccagt
     1921 tcgtgtcgag cgaactgttc gtccttgccg cctgcgatga ccgcggtgaa ttgggccggc
     1981 tcctcaaggc gaacggggca acccgcgcgg cgatcgagaa ggccatcgac gaggtccgcg
     2041 gcggagccaa agtggaagac gcgggcgccg aggagaatcg tcaggcgctc gaaaaataca
     2101 gcatcgacct gaccgagcgc gcgacctccg ggaaactcga tccggtgatc ggccgcgatg
     2161 acgagattcg ccgcaccatc caagttctac aacgccgtac caagaataat ccggtgttga
     2221 tcggcgagcc cggcgtgggc aaaaccgcca tcgtcgaggg cctggcgcag cgcatcgtca
     2281 acggcgaagt gccggagggc ttgcgcaaca agcggatttt gtcgctcgac ttgggcgcac
     2341 tcatcgccgg cgcgaagttt cgcggtgaat tcgaggaacg cctcaaggcc gtgctcaatg
     2401 atctggccaa gcaggaaggt caggtcattc tcttcatcga cgaattgcac accatggtcg
     2461 gtgccggcaa ggccgagggt gccatggatg cgggcaacat gttgaagccg gcgctggcgc
     2521 gcggcgaatt gcattgcgtg ggcgccacga ccctggacga gtatcgcaaa tacatcgaaa
     2581 aagacgcggc gctcgagcgc cgtttccaaa aggtcttggt cgatgaaccc tcggttgagg
     2641 acaccatcgc gattttgcgc ggcctgaagg agcgctacga agtgcaccat ggcgtcgaga
     2701 tcacggatcc ggcgattgtc gcggccgcga ccttgtcgca tcgctatatc gcggatcggc
     2761 agctgccgga caaggccatc gatttgatcg acgaggcagg ttcgctgatt cgaatggaaa
     2821 tcgattcgaa gccggaggcg ctcgacaagc tcgaccggcg catcattcag ctgaagatcg
     2881 agcgcgaggc gctcaagaag gcgaccgacg aggcttcgcg caaacgcttg agcacgctgg
     2941 aagacgcgct caaggcgcta gagaaagaat attcggatct cgaggaagtg tggaaggcgg
     3001 agaaagcctc ggtgcagggc gccgcgcaca taaaagaaga gctggagcgc gcacggcagg
     3061 atgtggacac ggcgcgtcga gcccaggatt tggcgcgcat gtccgagctg caatatggtc
     3121 gcatcccggc gctcgagcag cggctcgcca aggcgctgga agtcgagcaa aagccgaatc
     3181 aactgttgcg caactccgtg accgaagtcg aggtggcgga gatcgtctcg aaatggaccg
     3241 gcattccggt atcgcgcatg ctcgagggcg agcgcgataa actgctgcgc atggaggaag
     3301 tgctcggcag gcgtgtcgtc ggccagggag aggcggtcac agcggtggcg gacgcgatcc
     3361 gccgaactcg tgcggggctt tccgacccgc accggccgag cggctcattc atgttcctag
     3421 gtccgaccgg cgtcggcaag accgagctgt gcaaggcgct cgcggaattc atgttcgaca
     3481 cggaagatgc gttggtacgg atcgacatgt cggagttcat ggagaaacat tccgtggccc
     3541 gcttgatcgg cgcaccgccg ggatacgtcg gctacgaaga gggcggttat ttgaccgagg
     3601 cggtgcgcag gcgcccgtac tgcgtcattc tgctggatga aatcgagaag gcgcatcccg
     3661 atgtgttcaa tgtgctgctg caggtgcttg acgacggccg cctcaccgac ggtcagggcc
     3721 gcaccgtgga tttccgcaat tgcgtggtga tcatgacctc gaacttgggt tcgcaggtca
     3781 tccaggaact ggcgggcgag gcgaattacg cgaagatgaa gcaagccgtg atgcaggtgg
     3841 tgggtcagca ttttcggccg gaattcatca atcgcgtcga cgagatcgtc gtgtttcact
     3901 cgctcgggcg tgagcagatc cggtcgatcg tcgacatcca gttgggacgc ttacggcagc
     3961 ggcttgcgga gcgcgatatc accctgcggc tggacgatgc tgcgcgcgac aagatcggcg
     4021 aagcgggctt cgacacggtg tacggagcgc ggccgttgaa gcgtgccatt caagcgcaaa
     4081 tcgagaaccc gttggcgcag aagattctga gcggcgattt tgtggccggg gaccgggtga
     4141 tcgccaaact caagaaggac gcgctggtgt tcgagaaaga acgcctgcac tgagcgcccg
     4201 ggtgcggcgc ggggccgccg ttcgctatac ttgcggcgcg gctagggatc gacaacagga
     4261 agaacatgcc tttttacgag taccagtgca agaactgcgg ccacgatctc gaggccatgc
     4321 agaagatcag cgagccgccg ttgaagaagt gtccgcattg cggcaagccg cagctgcagc
     4381 gcctgatgtc ggcgccggtg tttcggctca agggcgccgg ctggtatgag accgacttca
     4441 agggcgacaa ggacaatcag cgcaacctcg cggatcggcc ggaggccgac ttgccggcag
     4501 aggtcgccaa ggatctcgcc aaagacacgg cgaaggaagg caccgccgat gccaaggcgg
     4561 gcgcgaacgg caaggatgtc aaggacgcca aagaggcaaa agctgagaag ggcgccgaca
     4621 aaccggtgga gaaagcagcg aaggagccgg ccaacaagcc gcaagctcgg cgcagcaaga
     4681 cgcgggcgaa gaagccgctg aggaaaccgg cgccgccggc gaggcggcga ggctagcatg
     4741 cgcacgcact attgcggaca ggtcaacgag tcgttgaccg gacaggtcgt cagcgttgcc
     4801 ggctgggcac agcgccgccg cgaccatggc ggcgtcatct tcgtcgatct gcgcgaccgc
     4861 gaagggctgt tgcaagtggt attcgacccg gacaaggccg agatgtttgc atcggccgag
     4921 cgcgtgcgca atgaattcgt gctcaaggtc acggggctgg tgcgggcacg gccgccgggc
     4981 accgccaatg ccaatttgaa gtcggggcag gtcgagttgc tgtgccagga actcaccatc
     5041 ctcaatcgct gcgagccgct gccgtttcag ctcgacgagc atgtgagcga agaggtgcgg
     5101 ctgcgctatc gctacatcga tctgcggcgc gaggagatgc gcgagcgctt gttgctgcgt
     5161 catcgaatta cgcgtgcgat gcgccactat ttggacgagg ccggctttat cgacatcgaa
     5221 acgccgatgc tctccaaggc gaccccggaa ggggcgcggg attacttggt cccgagccgc
     5281 acgcatgccg gcaaattctt cgccttgccg cagtcaccgc aaatttacaa acaattgctg
     5341 atgatcgcgg gtttcgaccg ctactatcag atcgtgcgtt gctttcgcga cgaggacttg
     5401 cgcgccgacc ggcaacctga attcacgcag ctcgacatcg agacgtcgtt tctcgatcag
     5461 ccggacatcc agaaattgat ggaaggtttg gtccggtatt tgttcaagca ggtgctcgcc
     5521 gtcgatttac cggacccaat accgcgtatg agctatgccg aggccatgcg tcgctacgga
     5581 tcggacaaac ccgacctgcg aatcccgctc gaattggtcg atgtggcgga tctggtgaag
     5641 ggctgcgatt tcaaagtgtt tgcgggtccg gcggccgatc ccgacggacg cgtcaccgca
     5701 ctgcgtgtgc cgcaaggcgc acgattatcg cgcaaggaga tcgatgacta caccgcctac
     5761 gtggcgcgct atggcgcgaa aggcctggcc tacgtcaagg tcaatgaggt ggcgaaacaa
     5821 cgcgaaggat tgcaatcgcc cattttgaaa ttcctgagcg atgcggcggt tggcggaatt
     5881 ctcgagcgca ccggcgcggc cgacggcgac ttgattttct tcggcgccga cagccgcaag
     5941 gtggtcagcg atgcgttggg tgcgctgcgc ctcaaggtcg ggcaggatct caaactcggc
     6001 gcgacggggt ggcggccgct gtgggtcgtg gacttcccga tgttcgagtg ggacgcgcag
     6061 acgaagcgct ggcaagcgat gcatcatccc ttcaccagcc ccgccgattt ggactatgcg
     6121 gccttggcgg cgagccccgg cgaggcgacc gccaaagcct atgatctggt gttgaacgga
     6181 tcggaaatcg gcggcggctc agtgcgcatt cattcgcagg aactgcagtc cgtggtgttc
     6241 gatctgctgg ggatttcacc ggaggaagcg cgtctcaagt tcggatttct gttggatgcg
     6301 ctcaagttcg gcgcgccgcc gcatggcggc atcgcgttcg gactcgatcg gttggccatg
     6361 ctgatggccg gagcggacag cattcgcgat gtcatcgcct tcccgaaaac tcaaaccgca
     6421 gccgaccttc tgatggatgc gccgacggaa gtgagcgagg cgcagctgcg cgagttgcat
     6481 attcgcgtgc gcgtcccgcc tacgccctaa gtgaacgcag gaacctttcg gcgtccggaa
     6541 tcggttttga tcgtgatcta caccgagggc ggtgaattcc tattattgga gcggcgccgt
     6601 ccgcccggtt tttggcagtc cgtgacgggc agcttggaat ggggcgaatt cgccgacgct
     6661 gcagcacggc gcgaagtggc ggaggaaacc ggcatcacgc aaggcgtgct cgtcaatcta
     6721 cagtggacgc aggtgtatga catcttggcg tcgttcggca aaatttacgc gcccggaatc
     6781 actcgcaatc tcgagcacgc tttttccttg cgactcgccg ggcgcgtgcc ggtgacattg
     6841 agcgactcgg agcatgtcgg atatcgctgg gtgtcgggcg aggaggctgt cgaaacggta
     6901 tcctctagca ccaatcgggc ggtgatcgag gacctgcgtt tgggagtcgc gccgtgagta
     6961 ccgtcatcgt gtacgtgcac ggattgtggc agcgcggcgc tgaatcgctg tggctgcggc
     7021 ggcgcttggc gcaagatctg gacgccgacg cgcgcatctt ttcctacccg tcggttgccg
     7081 ccgatgcgac cgccaattcg cgggcgctcg ccgaatatct tagcgccatc ggcgccgata
     7141 cactgcactt gatcggacat agcttgggag gcctcgtgat cctcaagctg ttcgaggaaa
     7201 tggcgcaggc gcgcgcgcac tggccgcccg gacgaattgt gctgctgggt ccaccgttgc
     7261 gcggcagccg aacggcgcaa aatctggcac gcatcccatt tggcaagaga ataatgggtc
     7321 taggggtgag cgaggaactg ctggtgccgc gcgagcgcac ctggtcggga tcgcgggatt
     7381 tgggtgtagt ggtgggcgat ttgggggtgg ggctcggccg gctggcgggg tctcttgggg
     7441 gtccgaacca cggaaccgtg ctggtcgagg agacgcagct gcccggcgcg accgatcgcg
     7501 tggtgctgcg cgtaagtcac accgggatgc tgttttcggc agcggtggcg cgtcaggctt
     7561 gcgtattctt gcgcaccggc cggtttgcac attgattctc gctgcgcgct agccgtgctc
     7621 gcctcggccg ccgcggtcga gggtttgtag ccgaaacagc aagtcgagcg cttcgcgcgg
     7681 gctcaaggca ttggggtcga gcgcgcgcag ggcctcgagc gctgcggatt cgggcgccgg
     7741 cgcggatgcg ggtgcaaaca ggggcagttc ggtttgcggc gaagtggagg tgcgcagcgc
     7801 gtcgcgctcg cgttcgagtt cgctcaagta acgccgcgcc tgcgcggtga ccgatttcgg
     7861 gatgccggcg agagccgcga cttgcaagcc ataactctgg ttggccgggc cgtccttgac
     7921 gctgtgcaag aagaccaacg tgtcgccgtg ttccaccgct tcgacgtgca cattgaccac
     7981 ccccggggcc tcgccggcga gactggtgag ttcgaagtag tgcgtggcga ataaggtgaa
     8041 tgcgcggatt ttattcgcga tgaacgccgc gcaggcccag gcgagggaca gtccgtcgaa
     8101 tgtgctggtg ccgcgtccca cctcatccat gagcacgaga ctttgcgccg tggcattgtt
     8161 cagaatgttt gccgtctcgg tcatctccag cataaaggtc gagcggccgc cggcgagatc
     8221 gtccgacgcg ccgatccgcg tgaagatgcg atccatcgga cccagcacca cgcggcgagc
     8281 cggcacgtag cagccgatgc gtgccagaat cacgatcagc gccgtttggc gcatataggt
     8341 gctcttgccg cccatgttcg gccccgtaat gatgagcatg cggcgcgaat catcgcagcg
     8401 caggtcattg ggaacgaagg gttctcggcc ggcgcgctcc accaccggat ggcggccggc
     8461 ctcgatcagc aacatcggtt cgtcgacgag ttcgggctgc gcgcagtcga gagcggcggc
     8521 acgctctgca aaacacgcaa gcacgtcgat ttgcgcgata gcggccgtgc tcgcctgcag
     8581 cgccgccagg cgcgacgtca aatggtcgag taaaccttca tatagcgcct tttcccgggc
     8641 aagcgcccgg tcacgcgcgc caagtacttt gtcctcgaag gacttcaatt ccggggttat
     8701 gaatcgctcg gcggatttta ccgtctggcg gcgcaagtat tccgccggca ccgccgcagc
     8761 ctgactgcgg ctgagctcga tgtagaaacc ttgcacgcgg ttgaaaccca gcttgaggct
     8821 cgatagtccc gtgcgctcgc gctcgcgatg ttcgaggtcg agcaagaact gttcggtatt
     8881 ggtgccgagc agacgcagct cgtcgagctc cgcatcgaag cccggcgcga tcacgccgcc
     8941 gtcgcgcaga tggtgcggcg gagagtcgac gagcgctctc gacaacagcg cgtggtcttg
     9001 cgcgcgatcg tcattgtgct ctttgagttc gacgatcaac tgctgcagga gcggcgaggc
     9061 ggcgctgcca accgcggctt gcagctgcgg caggacgccg agcgcggcgc gcagctgcgc
     9121 gaggtctcgg ggcctggcag aacgcagcgc gattctcgcg acgattctct ccaaatcgcc
     9181 gattgcctcg agaggctgcg cgacggccgt gtgcagcgcg ccttccatca gcgtcgcgac
     9241 cgcgtggtac cgggcacgca atacaccgtt atcgcgcagc ggccggtgca gccaacggcg
     9301 cagcatgcgc ccgcccatgg cggttgcggt gcgatcgaaa acgcccgcca gggtcagttc
     9361 tgcgttgccg gcaaggctct cgtcgagctc gaggttgcgc cgcgtcgccg ggtccatgat
     9421 gagcgcggca tcgcgctctt ccacagctat cgatagcaga tgcggcagcg cggatttctg
     9481 cgtgtcgcgc acgtaggcga gcagcgcgcc ggccgcggct atcgccaacg gcttgtcggc
     9541 gcagccgaat cccgcgaggt cgcgcgtgcg aaattgttcg gtgagggcgc gcgtcgcaga
     9601 ttcggtatcg aaatgccacg gcggccgcag acggaacggc cgctcggaca gccgctctgc
     9661 cccctccggt gccaacaatt cggcgggacg cagccgttcg atctccgcct cgagcgccgc
     9721 cgcccccggc agttccatga tggtgaagcg gccggctgcg aggtcgagcc aggcgagacc
     9781 gtaacggcca ttggcgccga acacgctggc gagcaggttg tcgcggcgtt cgtcgagcag
     9841 agcttcgtcg gtaatcgtcc ccggcgtcac gatgcgtacg acttcgcgct ccatcggacc
     9901 cttggttttt ccgggctcgc cgcgttgctc gcaaatagcg accgactcgc ccttgcggac
     9961 caatcgcgcc agataggcgt cgagcgtgac cgcgggcacc ccggccatgg ggatgggcgc
    10021 gccggcggat gcgccgcgct gggtgagcgc gatatccagc agctgcgcgg cacgccgggc
    10081 gtcgtcgtag aacatctcgt agaaatcgcc catgcgatag aacaagagca cgtcgggatg
    10141 ctgcgccttg atgcgcaggt actgctgcat catgggggtg tggtcggggt gctgtgagcc
    10201 aggtccggca aaatctgttt ctttctgata tttcatgtag ttagtagaat atag
//
LOCUS       DASWZT010000457         5562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_23200, whole genome shotgun sequence.
ACCESSION   DASWZT010000457 DASWZT010000000
VERSION     DASWZT010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23200"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..737
                     /locus_tag="VGE92_13045"
     CDS             219..737
                     /locus_tag="VGE92_13045"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY1314800.1"
                     /translation="MALWNEKTSPAPAGSAAPASPAPPERAPERSAVDTQSLAARRPT
                     PKTSSEGGESVIAANLTIEGKIEGSGNVRMAGRFKGDVRIDGNFSIEPGAHLTGQVLA
                     GIVVVGGELQGNIESAKRVDVTEGGVIVGDVKAGSITVAAGSRMRGHVEFGWEEEKEK
                     VKVKPGLVSSGS"
     gene            899..1093
                     /locus_tag="VGE92_13050"
     CDS             899..1093
                     /locus_tag="VGE92_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314801.1"
                     /translation="MRIDGLIRHAERDESCEYCVVISITNERGEKLQRQVVGVGALAP
                     GEKHKISFSVDLMPVRGRRA"
     gene            complement(1104..1706)
                     /gene="folE"
                     /locus_tag="VGE92_13055"
     CDS             complement(1104..1706)
                     /gene="folE"
                     /locus_tag="VGE92_13055"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEY1314802.1"
                     /translation="MAKKKNAKVTRADAEGAVRTLLRFAGDDPLREGLRDTPKRVVNA
                     YSDWFSGYRIDPGAYLRRTFEEVEGYDEMVVLRDIGFESFCEHHMAPIIGRAHVGYLP
                     TARVVGISKLARVVDGFARRFQVQEKLTSQIAGCINDVLKPRGVGVVIDAVHQCMTTR
                     GVHKRGVSMITSKMLGTFRADASTRAEFLRFIDIEDRSPR"
     gene            1783..2649
                     /locus_tag="VGE92_13060"
     CDS             1783..2649
                     /locus_tag="VGE92_13060"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY1314803.1"
                     /translation="MAILQLLESRQRDLGGFTVRRVLPATLRQMVGPFIFFDHLGPTR
                     FAPGFGIDVRPHPHIALATVTYLFAGSLEHRDSLGSVREIRPGDVNWMSAGHGIAHSE
                     RTPRAARASGEDVHGIQSWVALPDGQEDGRPTFVHYPAASLPRRVADGVDLTVVAGEA
                     FGLQSPVVTLWPTLYVHAQFSPGAGLEVAADQTERAVYVIQGELAIDDHLLTPGQLAV
                     LEPGTTPALRAPGAGRAMLLGGEKFPTPRYIWWNFVASSQERIERAKERWERQEFPAV
                     PGETEFIPLPHY"
     gene            2666..3103
                     /locus_tag="VGE92_13065"
     CDS             2666..3103
                     /locus_tag="VGE92_13065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HEY1314804.1"
                     /translation="MAAKTASVHWEGQGKKGVGKISTQTDALKSYPYGYGSRFEDDRR
                     GTNPEELLAAAHAACFTMAFSFACDAAGYATAAVDTQAAVRLVMKGNGFLIDRIALRL
                     EATVPGIDEATFQKLAAAAKQDCPLSKALASVPEVSLTAVLRR"
     gene            complement(3118..3918)
                     /locus_tag="VGE92_13070"
     CDS             complement(3118..3918)
                     /locus_tag="VGE92_13070"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY1314805.1"
                     /translation="MIEAASIENFQRDGAICIRQLFRPQEISVLRSGIDENLAHLSPR
                     AKVASGADDPGRFVEDFCNWQDNGHYRRFIFDSALAETAGRLMKSTTARLYHDHMLTK
                     EPGTRQPTPWHQDQPYYNIEGRQNVSFWIPVDPVSRESTLEFVAGSHLGPWLMPRSFM
                     DAQAKWFPEGSLADLPNIDAARDAFPILGWALEPGDAVCFHMLTLHAARGVSGSDRRR
                     VFSVRFLGDDVRHAPRRWRTSPEFPGLREALPAGAPMIHPLFPELWRA"
     gene            complement(3915..4904)
                     /gene="pyrD"
                     /locus_tag="VGE92_13075"
     CDS             complement(3915..4904)
                     /gene="pyrD"
                     /locus_tag="VGE92_13075"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003652.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase (quinone)"
                     /protein_id="HEY1314806.1"
                     /translation="MPLLTRLGPELAHDVGLAGLRLLRPLWTAPRFPDVLTVECLGLR
                     FAHPLGLAAGFDKNGDCLDSLGAMGFSHVELGTVTPRPQPGNPKPRMFRIPEANALIN
                     RMGFNNKGVDHLVKRVFRSSYRGIRGISIGKNFDTPLDHAQNDYVTCLRKVYALADYI
                     AVNISSPNTARLRELQTHDGLERILSALFEERRVQQVRYGKRVPLLVKVAPDLSEEEI
                     YALARDLRALEVDGVIATNTSTDHSAVGDASAAGQLGGLSGAPLHRLSVRVISQLRAE
                     LGDGFPLIGVGGILSAEDARATLRAGANLLQIYTGFAYRGPALVEEIVNSLPL"
     gene            complement(4940..>5562)
                     /locus_tag="VGE92_13080"
     CDS             complement(4940..>5562)
                     /locus_tag="VGE92_13080"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY1314807.1"
                     /translation="WGIIPTTRRRQPFAGRWPGVLEIALTQQRYSVRVIEDCLNGRRT
                     VWEDPFKQGRNGLQGLAQRIEVNSPLALVMLMLGTNDFQSMHPHTAWHAAQGVATLVA
                     AIRQAPIEPDMPVPAILIIAPPPFNEPRGPIAAKFTGAPAKAVGLADAYRTVAGELHC
                     HFFDAGTVTSASAQDGIHLDADQHQTLGQALARCVAGLPGVLTPET"
BASE COUNT         1001 a         1744 c         1836 g          981 t
ORIGIN      
        1 tcgctagtcc gataaatggc ggcgcgtatc ctgcaggctg tacgaaaagt atgcgatttg
       61 ccggtttttg cccgtggcga ccctgtaggg gagccgcgga tgggtgcccc atggaaggct
      121 accgtctcca ttaaaagaca gtggtccgat gacattttcc ggcagtagcg tgtacgcttt
      181 gctcaaccat ctcaatggct cactgggaga tttaacacat ggccctttgg aacgaaaaaa
      241 cctcgcctgc ccccgcgggt tctgcggctc ccgcgtcgcc tgcgccgccg gaacgtgcgc
      301 ctgagcgcag cgcggtcgat acgcagagtt tggcggcacg ccgccccacc cccaaaacct
      361 cctccgaggg cggggaatcc gtcattgccg cgaacctcac catcgaaggc aagatcgaag
      421 gctccggcaa tgtgcgcatg gcgggccgat tcaagggcga tgtacgcatc gacggcaatt
      481 tcagcatcga accgggtgcc catctgaccg gccaagtgct ggccggaatc gtggtcgtcg
      541 gcggcgagct gcagggcaac atcgaatccg ccaagcgggt ggatgtgacc gaaggcggcg
      601 tcatcgtcgg tgacgtcaaa gcgggatcga tcacggtggc cgccggttca cgcatgcgcg
      661 gacatgtcga atttggctgg gaggaagaga aagagaaggt caaggtcaag ccgggcctgg
      721 tgagcagcgg cagctaacca tggccgtcgc tcgggctggc gcgttagggg caacgcgagt
      781 atgtccgcac tgcaaagccg tagtgctgca gagcgcgaac gtatgtccgg gttgccaaca
      841 ccacctgcgg ttcaattctg ccgcgaattc cgaaccgcaa gcggaatcgg tatcggcgat
      901 gcgaatcgac ggattgattc gccacgccga gcgcgacgag agttgcgagt actgcgtggt
      961 gatttcgatc accaacgagc gcggtgagaa gttgcaacgc caagtggttg gcgtcggggc
     1021 gctggcgccc ggcgagaagc acaagatcag cttctcggtc gacctgatgc cggttcgcgg
     1081 ccggcgcgcg tagcgccgat cgatcagcgg ggactgcggt cctcgatatc gatgaagcgc
     1141 aggaactccg cgcgcgtgct ggcatcggcg cggaaggtac ccagcatttt actcgtgatc
     1201 atggagacgc cgcgtttgtg cacgccgcgc gtcgtcatgc actgatggac cgcgtcgatt
     1261 acgaccccga cgccgcgcgg cttgagcacg tcgttgatgc agccggcgat ttgcgacgtg
     1321 agtttctctt ggacctggaa gcggcgcgcg aaaccatcga ccactcgcgc aagtttgctg
     1381 atgccgacga cgcgcgcggt aggcaggtag ccgacgtgtg cgcgtccgat aatgggcgcc
     1441 atgtggtgct cgcagaacga ttcaaagccg atatcgcgca gcaccaccat ctcatcgtag
     1501 ccctcgacct cttcgaaggt gcggcgaaga tacgctcccg gatcgatgcg atagccggag
     1561 aaccaatcgc tgtaggcgtt caccacccgc ttcggcgtgt cgcgcaagcc ctcgcgcaaa
     1621 ggatcgtcgc cggcaaagcg cagcagcgtg cgcaccgcgc cctcggcgtc ggcacgggtc
     1681 actttggcgt tttttttctt ggccatgcgc gcagaatacc atcgttaggg gtgcggccag
     1741 gaaggtgcgc ggccgccgcc ggccggagta tgcttcggct ccatggccat cctgcaattg
     1801 ctggaatcga ggcagcgcga tctcggcggg ttcaccgtcc ggcgcgttct gcctgcgacg
     1861 ctgcggcaga tggtcgggcc gttcattttt ttcgatcatc tcggcccgac gcgcttcgcg
     1921 ccgggcttcg gcatcgatgt gcgcccgcac ccgcacatcg cgctggcgac ggtcacctac
     1981 ctgtttgcgg gctcgctcga gcaccgcgac agcctgggtt ccgtgcgcga aatccgaccc
     2041 ggtgatgtga attggatgtc tgccggccac ggcatcgcgc actccgaacg cacgccgcgc
     2101 gcggcgcgcg cgagcggtga agatgtccac ggcattcaaa gctgggtggc gttgcccgat
     2161 gggcaggagg atgggcgacc gaccttcgtg cattaccccg cggcgagtct gccgcggcgc
     2221 gtcgccgacg gggtcgattt gaccgtcgtc gccggcgagg cgttcggact tcaatccccg
     2281 gtcgtaaccc tgtggccgac gttgtatgtt catgcgcagt tttcgcccgg cgccggcctc
     2341 gaggtagccg cggatcagac cgaacgtgcg gtgtacgtga ttcaggggga actcgccatc
     2401 gacgatcatt tgttgacgcc gggccagctt gccgtgctcg aaccgggcac gactccggcg
     2461 ctgcgtgcgc cgggcgccgg ccgcgccatg ctgttgggcg gcgagaagtt tccgacgccg
     2521 cgctacatct ggtggaattt tgtagcttcc tcgcaggagc gcatcgagcg cgccaaagag
     2581 cgctgggagc ggcaagagtt tcccgccgtg cccggcgaaa ccgaattcat tccgttaccg
     2641 cattactgaa accaaggagc ctgcgatggc tgccaagacc gcgagcgtac attgggaggg
     2701 gcagggcaaa aagggcgtcg gcaaaatcag cactcagacc gatgcgttga agtcctatcc
     2761 ctatggttac ggcagccgct tcgaggacga ccgccgcggc accaatcccg aggagttgct
     2821 ggcggcggcc catgccgcgt gcttcacgat ggcattctcg tttgcctgcg atgcggcggg
     2881 atacgcaacc gccgccgtcg acacacaggc agcggtgcga ttggtgatga agggcaatgg
     2941 gtttttgatc gatcgcatcg ccctgcgcct ggaggcgacg gtacccggca tcgatgaggc
     3001 aacgtttcag aaactggccg ccgctgcgaa gcaggactgt ccgctgtcga aggcattggc
     3061 cagcgttccc gaagtctcgc tcacggcggt tttgcgccgc tgaggcgcag ccgccgatca
     3121 tgcccgccac agttccggga acagcgggtg gatcatcggc gcgccggcgg gcagtgcttc
     3181 gcgcaaaccc ggaaattccg gcgaggtgcg ccagcggcgc ggcgcgtgcc tgacgtcatc
     3241 gcccaagaat cgcaccgaaa aaacgcggcg gcgatcgctg ccgctgacgc cgcgggctgc
     3301 gtgcaacgtg agcatgtgaa aacagacggc atcccccggc tccagcgccc agccgagaat
     3361 cggaaatgcg tcgcgggcgg catcgatatt cggcagatcg gccagactgc cctcgggaaa
     3421 ccacttcgct tgtgcgtcca tgaaggagcg cggcatcagc cagggtccca agtgcgaccc
     3481 ggccacgaac tcgagcgtcg attcgcggct gacgggatcg accggtatcc aaaagctgac
     3541 attttggcgg ccttcgatat tgtagtaggg ctgatcttga tgccacggcg tcggctgccg
     3601 ggtgccgggc tccttcgtca gcatgtgatc gtggtagagc cgggcggtgg tgctcttcat
     3661 caggcggcct gcggtttcag ccaatgccga gtcgaagatg aagcgacggt agtgcccgtt
     3721 gtcctgccaa ttgcagaagt cctcgacgaa acgtccggga tcatccgcgc cgctggcgac
     3781 cttggcgcgc gggctcaaat gcgccaggtt ctcgtcgatt cccgaccgca gcacgctgat
     3841 ctcctgcggc cgaaacagct gccggatgca aatcgcgccg tcgcgttgaa agttctcgat
     3901 gctcgccgcc tcgatcatag cggcaacgag ttgacgattt cttccaccag cgccggccct
     3961 cggtaagcaa agcccgtgta gatttgcagc agattcgcgc cagcgcgcag cgtcgcgcgg
     4021 gcatcctcgg cggacaagat accgccgaca ccgatgagcg ggaagccatc gcccaattcg
     4081 gcgcgcaact ggctgatgac gcgcacactc aagcggtgca gcggcgcacc gctcaagccg
     4141 cccaattggc cggcggccga tgcgtcgccg acggcactgt ggtcggtcga agtattggtc
     4201 gcgatcacac cgtcgacctc gagcgcgcgc aagtcgcgcg ccagcgcgta aatctcctcc
     4261 tcgctcaagt ccggcgcgac tttgaccagc aacggcacgc gcttaccgta gcgcacctgc
     4321 tgcactcggc gctcttcgaa caaggcgctc aggattcttt cgaggccgtc gtgggtttgc
     4381 agttcgcgca gccgcgcggt gttcggcgag gagatattca ccgcgatata gtcggcgagg
     4441 gcatagacct tgcgcagaca agtgacgtag tcattctgcg catggtcgag cggcgtatcg
     4501 aagttcttgc cgatgctgat gccgcgaatc ccgcggtagc tgctgcgaaa gactcgcttg
     4561 accagatggt cgaccccttt gttgttgaat cccatgcggt tgatgagcgc gttggcctcc
     4621 gggatcctga acatccgcgg cttgggattg cccggttgcg gccgcggcgt cactgttccg
     4681 agttcaacat ggctgaaacc catggcgccc agggagtcca agcaatcgcc gttcttatcg
     4741 aagccggccg cgagaccgag cgggtgcgca aacctcaatc ccaggcattc caccgtcagc
     4801 acgtccggaa atctcggtgc cgtccagagg ggccgcaata ggcgcagtcc cgccagtccc
     4861 acgtcgtgag cgagctcagg gccgagacgc gtgagaaggg gcaaggaggc tcccgcgaac
     4921 accgatgcgg cgcggcgcat catgtctcgg gcgtcaacac gcccggcaag ccggcgacgc
     4981 aacgcgcgag cgcttgcccc aaggtctggt gctggtcggc gtcgagatgg atgccatcct
     5041 gcgcgctggc gctcgtgaca gtcccggcat cgaagaaatg acagtgcaac tcgccggcca
     5101 cggtgcggta ggcgtcggcc agccccaccg ccttggcggg cgcgccggtg aacttggccg
     5161 cgatcggccc acgcggctcg ttgaagggcg gcggcgcgat gatgaggatg gccgggaccg
     5221 gcatgtccgg ttcaatgggc gcctggcgaa tggcggccac cagcgtggcc acaccttgcg
     5281 cggcatgcca tgcggtatgc ggatgcatcg actgaaagtc gttggtgccg agcatcagca
     5341 tcaccaacgc cagcggggaa ttgacctcga tgcgctgcgc gagaccttgc aggccgttac
     5401 gcccctgctt gaagggatct tcccagacgg tccggcggcc gttcaaacag tcctcgatga
     5461 cccgcaccga atagcgctgt tgcgtcagcg ctatttccag cacccccggc cagcggcccg
     5521 cgaacggctg gcgccggcgc gtggtcggga tgatgcccca gg
//
LOCUS       DASWZT010000458         2161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_90603, whole genome shotgun sequence.
ACCESSION   DASWZT010000458 DASWZT010000000
VERSION     DASWZT010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90603"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /locus_tag="VGE92_13085"
     CDS             <1..1274
                     /locus_tag="VGE92_13085"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1314808.1"
                     /translation="SNTAPGTPGNTGCLTNVGTAHGASVENPGEQNDSTAFFEDTQRQ
                     LKQLAEFASVDFDIIPKVLTVTAGTRHFRFTNTFTGSVTSSFGCYEAGVIPGGCVADA
                     TDLNAGNLSATESGSKSRGNITWHVTPDAMVYATWSQGFRPGAFNRQEASVAAYIDGP
                     DGKPQFAIPNYYKSDQLTNKEIGWKTEWFDHRFQWNGAVYQENWNNVQIGFFDPAETG
                     NLAFGDNGQDFRIRGVETSLVARLFQGLTVQGGASWNSSVQTNSPNLIDTNPSSVNFG
                     KPITEKCNSSGLNCVPISNLFGPEGSPSANSPPIQFNLRARYDWTMDTYNAFVQFGGQ
                     HVGHSFTQSGSNPAISAKGVTTTLLRFENPAYSDYDASAGVAKDNWNVHLFSQNLFNK
                     NVDLFTNTAQFVVAATPLRPRIIGIDFGYKF"
     gene            1339..>2161
                     /locus_tag="VGE92_13090"
     CDS             1339..>2161
                     /locus_tag="VGE92_13090"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1314809.1"
                     /translation="MQAAATTPTSSPVEVEVLRIRALLERNHFAAAMSAAEILAGKFP
                     ENRDVIYMIAVSQRGLRKIPEALATLERLLRLYPRFSRGFQERGHCYVALRDAPRATE
                     AFLTAVNINPALPASWRTLQSLYQMAGQPENAATAAAHVATLATLPAEIVTATALFSD
                     GELAPAEAIVRAYLLKHGNHVEAMRLLARIGLARDVLDDADVLLEAVLALAPDYQAAR
                     YDYAVALLRRQKHVQAVAELEKLLQADPNNRAYRTTHATARVGLGEHQAALEIYRE"
BASE COUNT          449 a          681 c          632 g          399 t
ORIGIN      
        1 cctcgaacac cgcaccgggg acgcccggca atactgggtg tttgacgaat gtaggcaccg
       61 cgcacggtgc cagcgtcgag aatcccggtg agcaaaacga cagtacggcg ttctttgagg
      121 acacccagcg tcaactcaag cagctggccg agtttgcgtc agtcgatttc gacatcattc
      181 caaaggtcct gaccgtgacg gcgggtacac gtcattttag atttacgaac accttcaccg
      241 gcagcgtcac cagcagcttc ggctgctacg aggccggcgt gattccgggc ggctgcgtcg
      301 ccgatgcgac cgatcttaac gccggcaatc tctcggcgac ggagtcggga agcaagagcc
      361 gcggcaacat cacttggcat gtaacgcctg atgccatggt ctatgccact tggtcgcagg
      421 ggtttcgacc gggcgctttc aatcgccagg aggccagcgt tgctgcttac atcgacggtc
      481 cggacggcaa accgcaattc gccataccca attattacaa atccgaccag ctgaccaaca
      541 aagagatcgg ttggaagacg gaatggttcg atcacaggtt ccagtggaac ggcgcggttt
      601 accaggagaa ctggaacaac gtgcagattg ggttcttcga cccagccgaa accggcaacc
      661 tggcattcgg cgacaacggc caggatttcc ggattcgcgg cgtcgagaca tccctcgtgg
      721 cgcgcctatt tcaagggctc accgtgcagg gcggcgcgtc gtggaattcg agcgtgcaga
      781 ccaactcgcc gaatttgatc gacaccaatc cttcgagcgt caatttcggc aaacccatca
      841 ccgagaaatg caatagctcg ggccttaact gcgtgccgat tagcaacctg ttcgggccgg
      901 aagggtcacc gagcgccaac tcgccgccga ttcagttcaa cctgcgcgct cgatacgact
      961 ggaccatgga cacctacaac gccttcgtgc agtttggcgg ccagcatgtc ggacattcgt
     1021 tcacgcagtc gggcagcaat ccggcgatct cggccaaggg tgtcacgacg acgttgctgc
     1081 gcttcgaaaa tccggcctat tcggactacg atgcctcggc gggtgtcgcg aaagacaact
     1141 ggaacgtgca tttgttctcg cagaacctgt tcaacaaaaa tgtcgacctg ttcaccaata
     1201 cggctcagtt cgtcgtggcg gcaactccgc tgcgtccgcg aattataggg atcgatttcg
     1261 gctataaatt ctaaggaccg gcagtcggag gcagtagaat aaaaggcggg cttgaggccc
     1321 gccttttcat tggaagtgat gcaagcagcc gctactaccc ccacctcatc ccccgtcgag
     1381 gtggaagtcc tgcgcattcg agcgcttctc gagcgcaatc atttcgccgc cgccatgagc
     1441 gcggcggaaa tcttggccgg caagtttccg gaaaaccgcg acgtcatcta catgatcgcg
     1501 gtgagccagc gcggcctgcg caaaatcccc gaggcgctcg ccactctgga gcggctgttg
     1561 cggctgtatc ctcgcttcag ccgcggcttc caggagcgcg ggcattgtta tgtcgcgttg
     1621 cgcgatgcac cgcgcgcgac cgaggcgttc ttgaccgccg tgaatatcaa tccggccctg
     1681 ccggccagct ggcgcacgct gcaatcgctc taccagatgg ccggacagcc ggagaacgcg
     1741 gcgacagccg ccgcacacgt cgcgacgctc gcgacactgc cggccgagat cgtcaccgcg
     1801 acagcactct tttccgacgg tgaacttgcg cccgccgagg ccatcgtgcg cgcgtatttg
     1861 ctcaagcacg gcaaccatgt cgaagccatg cggctgctgg cgcgaatcgg tctcgcccgc
     1921 gacgtgttgg acgacgccga tgtactgctc gaggcagtac tcgccctcgc accggattat
     1981 caggccgccc gctacgacta cgccgtggcg ctgctgcggc gccaaaagca cgtgcaggcc
     2041 gtcgccgagc tcgagaagtt gctgcaagct gacccgaaca atcgcgctta ccgcacgacc
     2101 cacgcgaccg cgcgagtggg tctcggtgaa catcaagcag cgctcgagat ctaccgcgaa
     2161 c
//
LOCUS       DASWZT010000459        19062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_56883, whole genome shotgun sequence.
ACCESSION   DASWZT010000459 DASWZT010000000
VERSION     DASWZT010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 19062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19062
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56883"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="VGE92_13095"
     CDS             <1..1227
                     /locus_tag="VGE92_13095"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1314810.1"
                     /translation="AMQGGGPILLAGINNQVEHNQTSQELQFSGHALDNALDWTGGAY
                     YYHGYSYNGGYVDVDFAGFGFSQANNSKLTDKSVFAHLDYKVGDLGIEAGLRYTKENK
                     SVTINEFVLPVSAGIPFVPQDTATTDLSRVDPKIGVRYNFTPDLMLYADYSTGFKSGG
                     INPRPTSAATTGIPFGAEKVQAYEVGLKSEWLNHTLRSNTALFLSNYDDFQIQGETGS
                     GNFILIVETAIGKARIYGVEEELQWEPVRGLNFNVSGGWLEFHTLSLGSSANCNCGGP
                     GLDTQPPGVPKVTANAGVQYELPVGAAGSLTPRLDYNYKSLVWNDSPNSSPTNTASPS
                     PYPNATPAIGLLDAQLTWRSAPLGGDRQGDWSVRAAVKNVTNKFYYQNTLLQPWTLDG
                     VPSLPRTWSVTVSRRF"
     gene            1426..3480
                     /locus_tag="VGE92_13100"
     CDS             1426..3480
                     /locus_tag="VGE92_13100"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY1314811.1"
                     /translation="MKDSLLDCLDGFFAVEWVGLCVDAMLRLREPHRDRLAAILLSLD
                     LPESHGLEAFHAILEVAQDVPILILSSVAREDCAKLAVQRGAQDYVLEDDLGSYLLPK
                     AVRNMIERAANAEALFSASERAEVTLNSIGDAVVSTDMCGNVTFLNPVAQTLTGWSAA
                     EAIGRPIDEVLQIVDNTDARCILSPMTTVVQTNRAAKLASGSRLIRRDAVELAIEDSA
                     APIHDRAGRVTGAVMVFHDVTQAQAISQKMSHLAQHDYLTDLPNRLLLNDRLSQAIAA
                     ARRSHLKLAVLFVDLDRFKHVNDSLGHAVGDELLLSIAARLVSCVRDSDTVSRQGGDE
                     FVILLSSIAHADDAAFSGQKLISAGALPHHIGGHNLQITLSVGISVYPDDGSDADTLI
                     KNADIAMLHAKDNGRNNFQFFKPSMNERAFERQSIESGLRHALERQQFVLHYQAKVDL
                     VTEMLTGVEALIRWRLPQRELICAGEFIPIAEQCGFIVPIGRWVLRQACIQARIWQDA
                     RLPSTPIAVNISAVELRSKHFVEHVRAILQETGAEPSWLEFELTETALMQDPQSTVGV
                     LKELKDMGIQLTLDDFGTGYSSLSYLRRFPIDALKIDKSFVHGLCTNADDCKIVSAVI
                     NLGRSFHLRVVAEGVETRAQFLALQSQNCGEGQGYYFRPAIAAREFTKLMGSDLSATD
                     AA"
     gene            3572..4201
                     /locus_tag="VGE92_13105"
     CDS             3572..4201
                     /locus_tag="VGE92_13105"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY1314812.1"
                     /translation="MTAIRNVIFDLGGVVLEWNPDQIVARFQPDAALRENFKKDVFGH
                     PDWQLFDRGALTELQMIDRIESRTGLPKNQAANIMDSVRESLVEKSATVRLMRSLHSR
                     GVTLFCLSNMPTSIYTHLRERHGFWDLFTGIVISGDVRMMKPEAAVFDYLLRKFSISA
                     AESVFIDDLAANIQGAIAVGLQGVLFQDARQCERDLNRLLLERASTAYP"
     gene            complement(4192..5079)
                     /locus_tag="VGE92_13110"
     CDS             complement(4192..5079)
                     /locus_tag="VGE92_13110"
                     /inference="COORDINATES: protein motif:HMM:NF007695.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEY1314813.1"
                     /translation="MVHISKAGTFKLGSRTVKRLGYGAMRLCGPGIFGPPKDKPGALA
                     VLREALERGVDHIDTSDYYGPHITNQLIREALHPYPKELVIVTKVGAKRGADASWKPA
                     FSAEDLTSAVHDNLRNLGVEALDVVNLRLMFGIHGPAAGSLEAPLSVLAKLQRQGLIR
                     HIGLSNVTPEQIAEGRAIADIACVQNHYNLANRADDALIEELTATGTAYVPFFPLGGF
                     TPLQSTALSEVAARLDVTPMQVALAWLLQRAPNILLIPGTSSVAHLRENLGVADLKLP
                     AEDVAVLDQIAAGSAPLHG"
     gene            5172..6080
                     /locus_tag="VGE92_13115"
     CDS             5172..6080
                     /locus_tag="VGE92_13115"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY1314814.1"
                     /translation="MTAELGDLTAFVAVARAGGFRDAARTRGSSASSLSEAVRRLETQ
                     LGVRLLNRTTRSVAPTEAGARLLDRLGPALAEVESALDVVNGFRDRPAGTLRLNVPVS
                     AARLVLPRIVPPFLAAYPEIKLDVIADDSFVDVLAAGCDAGIRYDERLEQDMIAIPIG
                     PREQRFATAASPAYLDRHGRPDHPRKLLEHACLLGRFPSGAMTAWEFERDGEVVKVEA
                     TGPLVVQVGAATDLAVDAALAGTGIITLFEDWLRPFLDSGALQPVIEPWWQRFSGPFL
                     YYAGRRYVPAPLRAFIDFTISHGRWT"
     gene            complement(6097..7185)
                     /locus_tag="VGE92_13120"
     CDS             complement(6097..7185)
                     /locus_tag="VGE92_13120"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEY1314815.1"
                     /translation="MRLARGLLLVVSLIGASAPAWSETRVALIIGNSRYADASLRLEN
                     PANDALAMDKALREAGFETVVKLNASRLDFYDAVNQFKAKISHDPDAVGLFYYAGHGV
                     QADGVNYLIPVDAKLESDSDLDARAYDAGRVLRAMEAAQNGMNIVILDACRDNPLPKS
                     RGAERGLARMTAASGTFIAYAAAPGQVAHDGSKGSNGVFTGELVKAMSVPGMPLEQVF
                     KRVIAGVKSDTKGSQQPWSESSIQGDFYFHAAAASTPVAHAVQSAAADDADVALWRSI
                     ESSRNAGDFRRYLDAYPHGLFAELARDRVKSLDVASSTARQPAHSTSPSATQAPVAST
                     DKKCQSLIEQAQLGEINEETKKALEKCH"
     gene            complement(7351..9282)
                     /locus_tag="VGE92_13125"
     CDS             complement(7351..9282)
                     /locus_tag="VGE92_13125"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY1314816.1"
                     /translation="MGYQVDDLTIDLAPRRVRRAGTAIPLKALSFDLLATLVRAAPNL
                     LSFDQLSEQVWPGLVITPETIVQRVKLLRDALGDDPHAPRYIEGVRGRGYRMVAEVRA
                     LTERQGTPECIVQPSNETFEEKQNPSEQAGVAITGSATSSLSATPSAAPRSARWVSLG
                     WIGGKLIIVGLLAASWAIVHYQGLGKPAERTSVVVSPVIGSLAVLPLDNLSGGKEQEY
                     FADGMTDALTTELAQIDSLRVISRTSALHFKGSKETLPQIGRELNVDAVVEGTVTRGE
                     NRVRVTAQLVETSSDHHLWARTYERDLKDVLALQDEIARDVAEQIRVKLTPKERSLRV
                     QVHPVDPEAYDAYLRGSYWVNQLTLEGYEKACDYFRKAVAKDPSYALAYVGVARCQDT
                     PDKYRDGIIKALALDPSLAEAHTRLGISKFLRDWDWPGAEAEHRQAIALNPNDASAHY
                     WYSFYLEAMGRPDEALREIERARDLDPYDAFTTVRLEQVLYHARRYDDALREDQRGLE
                     MHPNTDGFYWDMADVYEQKKMFAEAFAARQQALSLQKDPRVAALGEAYKRSGYKGYLF
                     KQAEFEQTRNTPYAAHCYALLNDERRAIAALEAAYNKREPGILFIRTAPELDSIRASP
                     GFRDLVRRIGFPPSPSDKN"
     gene            complement(10033..10566)
                     /locus_tag="VGE92_13130"
     CDS             complement(10033..10566)
                     /locus_tag="VGE92_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314817.1"
                     /translation="MKTTLMGIAATAALSLFGLPANANCAYPSAGNKEDFQLPAYHQH
                     FGDHEDIVGTWLVNYGPVGQAFIQWHNDGTEWENITHPAIGGNICMGSWVQTGRWTYS
                     RNHFGWIFDSKTGLIAGYFNETETDTLSKDGNSYAGTNVTIFYDMDHKVVPAPGAPSP
                     APTGGYPGTSSAVRIAP"
     gene            complement(11282..11590)
                     /locus_tag="VGE92_13135"
     CDS             complement(11282..11590)
                     /locus_tag="VGE92_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314818.1"
                     /translation="MKLASLVLLLCLTGCGWFHRKAPPPVTQQLIVTGVPVGSTLLVD
                     GKQTGEGDASNRSHVVDVVSGTHTVEVRMGDNVAYRESTYVAPGERRVVAVLSGDHRD
                     "
     gene            complement(11593..12036)
                     /gene="phaP"
                     /locus_tag="VGE92_13140"
     CDS             complement(11593..12036)
                     /gene="phaP"
                     /locus_tag="VGE92_13140"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Members of this family are phasins (small proteins
                     associated with inclusions such as PHA granules). Note
                     that several different families of phasins have been named
                     PhaP despite very little sequence similarity to each
                     other; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01841 family phasin"
                     /protein_id="HEY1314819.1"
                     /translation="MSSNDNSPFSGFDFSKFDVQKMLGDFKIPGVDMQSLLSAQEKNI
                     AALKQANQHAAEGLKALAKRQMEMMQQVMQGAASAAQQVAASSGPKEAAAKQAEVAKA
                     AFEQAVANMREMAELVSKSSSQAFEVITQRAAEGLEELKQLTTKK"
     gene            complement(12139..12990)
                     /locus_tag="VGE92_13145"
     CDS             complement(12139..12990)
                     /locus_tag="VGE92_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY1314820.1"
                     /translation="MRLVRYGAVGAERPGVLDQEGRIRDLGDRVPDIDAAALSPPGLA
                     RLRALNVGALPLVPGTPRFGACVARPHKFICIGLNYADHAREGGVAVPSEPEVFTKAT
                     SAISGPNDPIIKPRGSTKLDWEVELALVIGSVCRYADERAALASIAGYCVCNDVSERL
                     FQLERGKQWDKGKGCDSFGPIGPWLVTADEVDTSDLALWLDVNGKRQQSGSTRDMIFN
                     PVFIVSYVSQYMTLEPGDVISTGTPAGVGLGQKPPKYLNVGDTVKLGITGLGEQQQTV
                     VACDTAQ"
     gene            complement(13003..13737)
                     /locus_tag="VGE92_13150"
     CDS             complement(13003..13737)
                     /locus_tag="VGE92_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004827669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY1314821.1"
                     /translation="MQNARLSGKTALITAAGQGIGRAVAEQFAAAGATVWASDIRAEL
                     LADLTGCRPLQLDVRDKSAIAAAIQHVGDVDVLVNCAGYVHSGSILECDEEAWAFSID
                     LNVASMYRLASAVLPGMLARGRGSIINLSSVCGSIKGVPNRFVYGTTKAAVVGLTKSI
                     AADFVTRGVRCNAICPGTVDTPSLQERLRATGDYEAARKSFTARQPMGRLGTAPEIAS
                     LALYLAADESAFVTGQCHVIDGGWAN"
     gene            complement(13758..14774)
                     /locus_tag="VGE92_13155"
     CDS             complement(13758..14774)
                     /locus_tag="VGE92_13155"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY1314822.1"
                     /translation="MLKRGLRLSEIGLGGAPLGNLYRALDDDQARLTCRAALEAGISY
                     FDTAPFYGFGLSERRLGDALRGHPAYAVSTKVGRLLKPDPSIMDASPRQGYVSSMPFA
                     AVFDYTYDGVLNSWEASLQRLGLASIDVLYVHDIGAHTHGPMSEHFFSQLTAGGGFRA
                     LAELRSAGAIKAFGLGVNEIEVCRRAMDHADLDLVLLAGRYTLLEQPALDHLLPECQR
                     RQISLVIGGPYNSGILATGTRHGGPLYYDYGPAPPAVIDRVRRLESCCATFGIALAAA
                     ALQFPLAHPSVASVIPGLVSAAQIGETRALYATPIPVEFWSALKSQGLLRPDAPCPGA
                     AAGA"
     gene            complement(14792..15145)
                     /locus_tag="VGE92_13160"
     CDS             complement(14792..15145)
                     /locus_tag="VGE92_13160"
                     /inference="COORDINATES: protein motif:HMM:NF017174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEY1314823.1"
                     /translation="MSKRRCFALDLKADAALIREYCRLHEPGAVWPLVIAHIRSLGVE
                     DMQIWQRDDRLFMIMETSDDYPRPVADAKTGEASERWESLMAGFQRRLAHAAPDEKWA
                     SMRCIFELGAHLGEV"
     gene            complement(15138..16445)
                     /gene="fucP"
                     /locus_tag="VGE92_13165"
     CDS             complement(15138..16445)
                     /gene="fucP"
                     /locus_tag="VGE92_13165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HEY1314824.1"
                     /translation="MTDAGPGMPNARDAIVDRQYLRPFVLVTSLFFAWAFAAALNDVL
                     IRQFQKALALSRAESSVIQLAFYVGYFCAAIPAGLLIRRVGYKPTIILGLSLYAAGAF
                     LFYPAANMQRFGWFLAALYIIAFGLAFLETAANPYISILGKPSTAAARLNLAQSFYGL
                     GAIFGPIIGGLFIFSGIEPSAAQLGAMTPMELGAYRASESHAVQLPYVTMGIGIVLLA
                     LAVAWTPFPQLALEKSAARRSSPRSLWGVLRNPVLRYAVVAQFFYVGAQVTVWSFFID
                     FSKALLPNLPEKRAAFLLSASLGMLMLGRFSGAFIQARVRPERMLAWYGAANVVLCLV
                     AACASGWLAVGSLWMTSLFMSIMFPTIFALGVRELQEDTELGASFLIMSIIGGALMAP
                     LTGLLSEHMGGIHRAMCIPALCFLVCTGFALAFPALSNRARDV"
     gene            16520..17884
                     /locus_tag="VGE92_13170"
     CDS             16520..17884
                     /locus_tag="VGE92_13170"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEY1314825.1"
                     /translation="MGANRGLKHALLIIAIAVIAAGMHARVLAAPAAADAPSYTPAPS
                     NLAARDWFQDAKFGIFLHWGLYSELGGVGSQSLSEWIMNEARIPAAKYERLALFFNPR
                     SFDADTWVRQFKDAGARYIVITSKHHDGFAMFHSAVSPYNVVDATPFKRDPLAELAAA
                     CRRQNIKLFFYYSQLDWHNPDYFPRGRTGQSAGRPAAGDWSRYLDYQNAQIKELVTNY
                     GPIGGIWFDGWWDQEGTALQNSWDLGRTYRVIHDAQPAALIVNNHHQTPFPGEDYQTF
                     ERDLPGENGMGFNRAQVAQLPLEMSETMNGTWGFSLTDANYKSTQTLIRTLVGAAGRG
                     ANLLLNTGPMPNGQIQGENLQTLAEIGTWLRANGSSIYGTRAGPIAPRPWGVTTESKG
                     QIYVHILDWQDDTLFLPLRRPVKSAALITGASIPVRRVHDGIELTLPAARADDWDRIV
                     VLEK"
     gene            17892..18722
                     /locus_tag="VGE92_13175"
     CDS             17892..18722
                     /locus_tag="VGE92_13175"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY1314826.1"
                     /translation="MIDAHQHYWQLERGDYGWLTPDLTGLYRDFLPAQLQGTLADCGV
                     TGTLAVQAAPTEAETRFLFDLARRHASILGIVGWVDFEAHDVARRIDALMAEGGGLLI
                     GLRPMVQDIADPAWLDRDSLDRAFGVLTERELVFDALVTPVHLGFLERRLRRHPELRC
                     VIDHAGKPTPGARSLAAWSARLRSLANHTSAYCKLSGLLTQFEAPVTLDVLAPYVSRV
                     FDYFGPERIIWGSDWPVLTLRAAYRDWLDLSLELIRMHAPQAGAAIFADNARRAYRLD
                     "
     gene            18736..>19062
                     /locus_tag="VGE92_13180"
     CDS             18736..>19062
                     /locus_tag="VGE92_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fuconate dehydratase"
                     /protein_id="HEY1314827.1"
                     /translation="MIVITALETHDIRFPTSKELDGSDAMNADPDYSAAYVVLKTNCP
                     DGLCGHGFVFTIGRGNDIQTRACAALDYLVVGRRVEEVVSDLGAFARSLTGDSQLRWL
                     GPEKGVM"
BASE COUNT         3663 a         5881 c         5815 g         3703 t
ORIGIN      
        1 gccatgcagg gcggcggccc cattctgctc gccggaatca ataaccaagt cgagcacaat
       61 cagaccagcc aggaactgca gttcagcggc catgcgctcg acaatgcgtt ggattggacc
      121 ggcggcgcct actactacca cggctacagc tacaacggcg gctacgtcga cgttgacttc
      181 gccggcttcg gcttcagcca ggccaataat tcaaagctga cggataagtc ggtcttcgca
      241 caccttgact acaaagtggg cgacctcggc atcgaggcgg gcttgcgtta tacgaaggaa
      301 aacaagtccg tcacgatcaa cgaattcgtc ctgccggtca gcgccggcat tccattcgtg
      361 ccgcaagaca cggccacgac ggatctatcg cgggtggatc ccaaaatcgg cgtgcgctac
      421 aacttcacgc ccgatttgat gctgtacgcg gattattcca cgggcttcaa gtccggcggt
      481 atcaatcccc ggccgaccag tgcagcaacc acgggtattc cattcggtgc ggaaaaggtc
      541 caagcctacg aggtgggtct caagagcgag tggctgaacc acacgctgcg cagtaacacc
      601 gcactctttt tgagcaatta cgacgatttc caaatccaag gtgaaaccgg cagcggcaac
      661 ttcatcctca tcgtcgagac ggcgatcggc aaggcgcgca tctacggcgt cgaggaggaa
      721 ctgcagtggg agccggtgcg cgggctcaat ttcaatgtca gtggtggttg gctcgagttc
      781 cacaccttga gcttgggttc ctcggccaat tgcaattgcg gtggtccggg gcttgatacg
      841 caaccgccgg gcgttcccaa agtcaccgcc aacgcgggcg tgcagtacga actgcccgtg
      901 ggcgcggcgg gctcgttgac gcctcgcttg gattacaact acaagtcact cgtgtggaat
      961 gactctccca actcgtcacc caccaacacg gcctcgccct caccgtatcc caacgcgaca
     1021 ccggcaatcg gcttgctgga tgcgcaactg acgtggaggt ccgcaccgct cggtggggat
     1081 cgccagggcg attggtccgt gagggccgcg gtcaagaacg tgaccaacaa gttctattat
     1141 cagaatacgc tgctgcagcc gtggaccctc gatggggttc catcgctgcc ccgcacctgg
     1201 tccgtcacgg tctcgcggcg cttctgatcc atcggtgtaa ccctgaaact cagtgccgcg
     1261 cgcccactaa ctgcgggtaa ggtgcgttgg caacacccat tccatgtgcg ctaacagacg
     1321 gacggcgact tgcggatatg cgttaagtta cgctcaatcc ccaaggaccg tggaatgccc
     1381 agagcgattt tgctgatcca cgcagaggcc aagaaggcca cgctagtgaa agattcactt
     1441 ctcgattgcc tcgacggctt ctttgctgtc gagtgggttg ggctttgcgt ggacgcaatg
     1501 ctccgcttac gcgagcccca ccgggatcgc ctcgcggcaa ttttgttgag cctcgattta
     1561 cccgaaagcc acgggctcga ggccttccac gccatcttgg aggttgcaca ggacgttccc
     1621 atcctcattc tgagcagcgt ggcgcgcgag gattgcgcca agctggccgt gcaacgcggc
     1681 gcgcaagact atgttctcga agatgacctt ggcagctact tgctccccaa ggcagttcgc
     1741 aacatgatcg agcgtgctgc caatgccgaa gcgctattca gtgccagcga acgcgccgaa
     1801 gtgacgctca actcgatcgg cgacgccgtg gtcagcacgg atatgtgcgg caacgtgacc
     1861 ttccttaatc ccgtggcgca gacgcttacc ggttggtccg ccgcggaggc gatcggcagg
     1921 ccgatcgatg aagtgctcca gatcgtcgac aacacagacg cacgctgtat cttgagtccg
     1981 atgactacgg tagtccaaac gaatcgtgcg gctaagcttg cctccggcag caggctgatc
     2041 cggcgcgatg cggtcgagtt ggccattgaa gattccgctg cacccatcca cgaccgtgcc
     2101 ggccgcgtga ctggcgcggt catggttttc cacgatgtca cgcaggcgca agcgatatca
     2161 caaaagatgt cgcacctcgc acaacacgat tatctgacgg atctgccaaa tcgcttattg
     2221 ctcaacgacc ggctttccca agctatcgct gcggcccgtc gcagtcacct gaaactagcg
     2281 gtgttatttg tcgatctgga tcgtttcaaa cacgtcaacg actccttggg acacgctgtt
     2341 ggggacgaac tcctgctgtc catcgcggcg cggctggtga gctgtgtgcg cgactcggac
     2401 acagtcagcc gccagggcgg ggatgaattt gtgattctgc tgtcttccat cgcgcacgcc
     2461 gatgatgccg ccttcagcgg gcaaaaactg atctctgccg gcgccttacc ccaccatatc
     2521 ggcgggcaca atcttcaaat aaccttgagt gttggaataa gcgtctatcc ggacgatggt
     2581 agcgacgcgg atacgctcat caagaatgca gatattgcga tgttgcacgc gaaggataac
     2641 ggacgaaaca acttccaatt cttcaagccc agcatgaatg agcgcgcctt cgagcgacag
     2701 tccatcgaga gcggtctgcg ccatgccctg gaaaggcagc agttcgtact gcactatcag
     2761 gccaaagtag atttggtcac cgaaatgctg accggcgtcg aagcgttgat acgatggcga
     2821 ctgccgcagc gcgaactcat ttgcgccggc gaattcatac cgatcgccga acaatgcggc
     2881 ttcatcgttc caatcggccg ctgggtgctg cgccaggcct gtatacaggc ccgcatttgg
     2941 caggacgccc gcttaccgtc aactcccatt gccgtgaata tttccgccgt agaacttcgc
     3001 tcgaagcatt tcgtcgagca tgtgcgggcg attcttcagg aaaccggtgc cgagccgtcg
     3061 tggttggagt tcgaattgac cgagacggca ctcatgcaag atccacaatc aaccgtcggc
     3121 gtgctgaaag aactcaaaga tatgggcatt cagctcaccc tcgacgattt tggcacgggt
     3181 tactcgagtt tgagctattt gcgcagattc ccgatcgacg cgctcaagat agacaaatca
     3241 ttcgtccatg gtctgtgcac gaacgccgat gattgcaaaa tcgtcagtgc ggtgatcaac
     3301 ctcgggcgaa gcttccattt gcgagtcgtc gcggagggcg tggaaacacg ggcgcagttc
     3361 ctggcgctgc agagccagaa ctgcggcgaa ggacaaggat actattttcg cccggccatc
     3421 gccgcccgcg aattcaccaa gctgatgggt tccgatttgt ccgcgaccga cgcggcatga
     3481 cttggcgctc gatgagcgag ccacgcaatc cttgagcgat tcgaattgct cggtgcgaca
     3541 accgcgtgca gattccctta atattgtccc cgtgaccgcc attcgcaatg tgattttcga
     3601 tctgggcggc gtcgtgctcg agtggaaccc ggaccagatc gttgcgcgct ttcaacccga
     3661 cgccgcgctg cgcgagaatt tcaaaaagga cgtatttggt catccggatt ggcagctgtt
     3721 cgatcgcggc gcgctcactg aacttcagat gatcgaccgc atcgaatcgc gcaccggcct
     3781 gccgaagaac caggcagcga atatcatgga cagcgttcgc gagtccttgg tcgagaaatc
     3841 cgccaccgtg cgactcatgc gcagcttgca ttcgcgaggc gtgacgcttt tctgcctgtc
     3901 gaatatgccg acctcgatct atacgcatct gcgcgagcgg cacggatttt gggacctgtt
     3961 caccgggatc gtcatctcgg gcgacgtccg gatgatgaag cccgaagccg ccgtgttcga
     4021 ttatttgctg cgcaagtttt caatcagtgc cgcagagagc gtcttcatcg atgatctcgc
     4081 ggccaacatc caaggtgcca tcgcggttgg actgcagggc gttctgttcc aggacgcgcg
     4141 gcaatgcgaa cgtgatttga atcgtttatt gctcgagcga gcctctaccg cctacccatg
     4201 aagcggcgcg ctgccggccg cgatctgatc caacaccgcc acatcttcgg cgggtagctt
     4261 cagatcggcc acgcctaagt tttcacgcag atgcgcgacc gacgaggtgc cgggaatgag
     4321 caggatgttg ggagcgcgct gcagcaacca ggccaaagcc acttgcatcg gcgtcacatc
     4381 gagccttgcg gcaacctcgg agagcgccgt cgattgcagc ggcgtgaagc cgccgagcgg
     4441 aaagaaaggg acatacgccg tgccggtggc ggtgagttct tcgatcagcg cgtcatcggc
     4501 tcggttcgcg agattgtagt gattttgcac acaggcgata tccgcgatgg cgcgcccttc
     4561 cgcgatctgc tcaggtgtca cgttgctcag gccaatgtgc cggatcagac cttggcgctg
     4621 cagcttggca agcaccgaga gcggcgcttc gagcgaaccc gcggcgggcc catggatgcc
     4681 gaacatgagc cgcaggttga cgacatcgag cgcctccacg cctaaattgc gcaaattgtc
     4741 atgcacggca ctcgtcaagt cctcagcgga gaaggccggc ttccatgacg cgtctgcgcc
     4801 gcgcttcgcg cccactttgg tgacgatcac caactctttg ggataggggt gaagggcctc
     4861 gcggatgagc tgattggtga tgtgcggacc gtagtaatcg ctggtgtcga tgtggtcgac
     4921 gccgcgctcc agcgcctcgc gcaacaccgc gagcgcgcct ggcttgtctt tcggcggacc
     4981 gaagatgcca gggccgcaga gccgcatggc gccgtaaccc agacgtttca cggtgcgcga
     5041 gcccaacttg aatgtgccgg ctttgctgat gtggaccatg ggctctccta ggaatggacc
     5101 atcaatgtac accggcgcag cgcgtgcgat aatgggctct aatcggaaca ggctgtacgg
     5161 aagggcgcac aatgacggcg gaactcggcg acctcacggc gtttgtcgcc gtcgcacgcg
     5221 ccggcggctt tcgcgacgcg gcgcggaccc gcggcagcag tgcatcgagc ttgagcgaag
     5281 ccgtgcgccg cctcgaaacc cagctcggtg tgcggctgct caatcgcacc acgcgcagcg
     5341 tcgcgccgac cgaagcgggt gcgcgcctgc tcgatcggct gggtcccgcg ctggcggagg
     5401 tcgagtcggc gttggatgtg gtcaatggct ttcgggatcg gccggccggc acgctgcggc
     5461 tgaatgtgcc ggtgagcgcg gcgcgcctcg tattgccgcg catcgtgccg ccgttcctcg
     5521 ctgcatatcc tgagatcaaa ctcgacgtga tcgcggacga tagcttcgtc gacgtgctcg
     5581 cggcgggctg cgatgcgggg atccgctacg acgagcggct cgaacaggac atgatcgcca
     5641 tccccattgg gccgcgcgag cagcgcttcg cgaccgctgc ctctcccgcc tatctcgacc
     5701 ggcacggccg cccggatcat ccgcgcaagc tcctcgagca cgcgtgcttg ctcggccggt
     5761 tcccgagcgg cgcgatgacc gcctgggagt tcgaacggga cggcgaagtg gtgaaggtgg
     5821 aagcgaccgg tcccctggtc gtgcaggtcg gtgccgccac cgacctcgcc gtcgatgccg
     5881 cactcgccgg caccggcatc atcaccttat tcgaggattg gctgcgcccg tttctcgata
     5941 gtggcgcgtt gcagcccgtg atcgaaccct ggtggcagcg cttttcgggt ccgtttctct
     6001 attatgcggg gcgccgttac gttccagcgc cgctgcgcgc cttcatcgac ttcaccattt
     6061 cgcacggacg ctggacttga aggcagccgc agtacgctag tggcatttct cgagcgcctt
     6121 cttggtctct tcgttgatct ctccaagttg tgcttgctcg atgagtgact ggcacttttt
     6181 atcggtcgag gcgaccggcg cttgagttgc agagggactc gtcgaatgcg cgggctgacg
     6241 cgcggtcgaa gacgcaacgt cgagcgactt gacgcgatcg cgcgcgagtt cagcgaacaa
     6301 gccgtgcgga tacgcatcca agtagcggcg aaaatcgccg gcgttgcggc tgctttcgat
     6361 cgatcgccac agcgcgacat ccgcgtcgtc cgccgcggcg ctttggaccg catgcgcgac
     6421 gggcgtcgaa gccgccgcag cgtgaaagta gaaatcacct tgaatcgagg actccgacca
     6481 cggttgttgg ctccccttgg tgtcgctctt gacgccggcg atcactcgtt tgaatacctg
     6541 ctccaagggc atgccgggga cgctcatggc tttcaccagt tcgccggtga acacgccgtt
     6601 cgaacccttc gagccatcgt gcgcgacctg gcccggtgcg gcggcatatg caatgaaggt
     6661 gccgctggcg gcggtcatgc gggcaaggcc gcgctccgcg ccgcgggatt tcggcaacgg
     6721 attgtcccgg cacgcatcga gaatcacgat attcatgccg ttttgggcag cttccatggc
     6781 gcgcagcacg cgcccggcgt cgtaggctcg cgcgtctaaa tccgaatcgg attcgagttt
     6841 cgcatcgacg gggatcagat aattgacgcc gtccgcctga acaccgtgtc cggcataata
     6901 gaaaagcccg acggcatccg ggtcatgcga gatcttggcc ttgaattggt tcaccgcgtc
     6961 gtagaagtcc aagcggctgg cgttgagctt gacgacggtc tcgaagcccg cctcgcgcag
     7021 cgccttgtcc atggcgagcg cgtcgttggc gggattctcg agccgcagac tggcgtcggc
     7081 atagcggctg ttgccgatga tcaaggccac gcgcgtctcc gaccacgccg gcgccgacgc
     7141 gccgatcagc gacacaacga gaagcagtcc acgcgccagt cgcacgttca tccctcccat
     7201 ccccatccta gggacgtccg cgaaagcacg aatatctgct gcactttcat tttacaagct
     7261 gcctcgagga ttgggaaatg gggcattgcc gaggatgaac ccgcgctcga ttgttggcac
     7321 gcgccgcaat ccggtttctt ggaagcggat tcagttcttg tcgcttggcg atggcgggaa
     7381 cccgatgcgg cggaccaggt cgcggaaacc tggcgatgca cgaatggagt ccaattcggg
     7441 ggcggtacga atgaacaata tgcccggttc gcgtttgttg tacgctgcct ccagggcggc
     7501 aatggcgcga cgttcgtcgt taagcagcgc ataacagtgt gctgcataag gtgtgttgcg
     7561 tgtttgctcg aattcggctt gcttaaacaa atagccttta taccccgaac gtttgtacgc
     7621 ctcaccgagc gcggcgaccc tcggatcctt ttggaggctt agcgcttgct gacgcgcggc
     7681 gaaagcctcg gcaaacatct tcttctgttc gtatacatcg gccatgtccc agtagaagcc
     7741 gtcggtgttt ggatgcattt ccaaacctcg ctggtcttcg cgcaacgcat cgtcgtaacg
     7801 tcgagcgtgg taaagcactt gttccagcct gacggtggta aaagcgtcgt acgggtcgag
     7861 gtcccgggcg cgttcaattt ccctcagggc ttcatcaggc cgccccatag cctctaagta
     7921 gaacgaatac caataatggg cagaggcgtc gttgggattg agggcgatgg cttgtctatg
     7981 ctcggcttcg gcgccgggcc agtcccaatc cctgaggaac ttgcttatgc cgagccgcgt
     8041 gtgggcttcg gcgagcgacg gatcgagcgc cagggctttg atgatgccgt cccggtattt
     8101 gtcgggagtg tcctggcacc gcgcgacacc gacataggca agcgcatagc tcggatcttt
     8161 ggcaacagct ttccggaagt agtcgcaagc tttctcgtat ccttccaagg taagctgatt
     8221 cacccagtac gagcccctca gataggcgtc atacgcttcg ggatccacgg ggtgaacctg
     8281 cacgcgcaga ctgcgctctt tgggagttag cttgacgcgg atttgctcgg caacatcccg
     8341 ggcgatttca tcctgcaacg caaggacgtc cttcaagtcg cgttcgtagg tcctggccca
     8401 caaatgatga tcactgctgg tttcgactaa ttgcgcagtc acccgcacac gattttcgcc
     8461 ccgtgtcacc gtgccttcca cgaccgcatc tacgttcaat tcgcggccaa tctgcggcag
     8521 cgtttccttg gaacctttga agtgcaacgc ggaggttcgc gagatgaccc gcagcgaatc
     8581 gatctgagcg agttcggtgg tcaacgcgtc ggtcatcccg tcggcgaagt actcctgctc
     8641 cttgccaccc gagagattgt cgagcggcag caccgccagc gagccaatga ccggagagac
     8701 cactacgctc gttcgctcag ccggtttgcc gagcccctgg taatggacga tcgcccagga
     8761 ggccgcgagc agccccacta tgatcagttt tccgccgatc cagccgagtg aaacccatct
     8821 cgcagagcga ggagctgcag atggcgtggc ggagagggag ctcgtggcag atcccgtgat
     8881 tgcgacgcca gcctgctcgc ttgggttttg tttttcttcg aacgtctcgt tcgaaggctg
     8941 cacgatacat tcgggggtac cctgtctttc ggtcagcgca cgcacctcgg cgaccatgcg
     9001 gtagccgcgg ccccgcaccc cttcgatgta gcgtggcgca tgcgggtcat cgccaagagc
     9061 gtcgcgcagc agcttcacgc gctgaacgat cgtttcgggc gtgatcacaa ggccgggcca
     9121 tacttgctcg ctcagctggt cgaagctgag gaggttgggc gcggcgcgca ccagcgtggc
     9181 aagcaaatcg aaagacaggg ccttcagtgg aatggccgtt cccgcgcgcc tcacccgccg
     9241 cggtgccagg tcaatggtca gatcatcgac ctggtacccc acgggatcgg gggtcggctt
     9301 cggggtattc gtactccact cttggggcgc cacggtggaa ctctctgctg gccgtctcct
     9361 gccgcagttg tggcgccatg ttacaccgga gcgcgcgccc gcgccctccc ggcgcaaaag
     9421 gctggagccc gggatctcgc acggcgatgt acccctccgt aaccctacgt ggcccctagg
     9481 gccgccggac ttaaataaat gtccagcaag ggggttgaag atgagcctta cggttgaagc
     9541 gcttgatcac ctcgtgatta acgttcgtga cgtggaagtt tcggctaggt ggtatcgaga
     9601 cgtgcttggc atggtccgtc aagaatctgg cccgaagcca ggagagaagc cgcggacatc
     9661 gcttaaattt ggaaaacaga aaatcaactt acgtcctgtg acgatatgta aagaggtgtg
     9721 gttcacggcc gagaacgaga ccgcggggag ccatgatctt tgcttcctca cccaatccaa
     9781 tcctgatgtc gttatcaagc acctgggctc tcgcggaatc gccgttgaga aaggaccttt
     9841 cggaaggaag gggcaatggg agagctgatc tcgatttatt gtcgtcgtca gcgcctgttg
     9901 cgcctcgggc gctgcgcctc aaattcggcc cgcttaccac catgagcacc aattacatcg
     9961 ccgtacctgg catggctccc gccgtcgcgg acttcgcgac gacgggagcc tgaggtccgc
    10021 gatcaattga tactacggcg cgattcgaac cgccgaggac gtaccaggat atccacccgt
    10081 tggtgccggc gacggtgctc ccggtgccgg caccactttg tggtccatgt cgtagaaaat
    10141 cgtaacgttg gttccggcat atgagttccc atctttcgac agtgtgtcag tctcggtctc
    10201 attgaaatat ccggcgatca atccggtctt cgaatcaaaa atccaaccga agtgattgcg
    10261 ggaataagtc catcggcccg tctgtaccca agaacccata cagatgtttc cgcctatggc
    10321 ggggtgagtg atgttttccc actcagtgcc atcgttgtgc cattgaatga aagcttggcc
    10381 aacggggccg tagttcacaa gccaggttcc cacgatgtcc tcatggtccc cgaagtgctg
    10441 gtgatacgca ggaagttgga aatcctcctt attcccggcg cttgggtacg cgcagttggc
    10501 attcgcaggc agtccgaaaa gcgataaggc tgccgtagca gcaattccca tgagagttgt
    10561 tttcataaag ttagcctccg agtaattttg ttgcgttgca gagcgttttc tgatggcatg
    10621 acatggctcg gtaatacgta gcacttccct accaaagtgg ttcttcctcg cattcttctg
    10681 tttatggtca tctcccggtg ggttgttagg caccgctgaa aatgctatgt gccccaaagc
    10741 ggcggtgcat gaaactgggc ccaacattgg tgaaggtgga tgaagactgg tgaagcttgg
    10801 tgaagcgtgg cggtccctca ccgctttgcg taccgtatgg ctgctttgga gaagttaccg
    10861 cgacgacgac gaatgtcggt cgatcggaga cccgtgacta aaaggctgga gcccgggatc
    10921 tcgcgcggcg atgtatccct ccgtaacccc acgaggcccc acgtaccgcg acaattcaca
    10981 cgccaatcac acttggcatc agcattcgca cgaaaccgcc tgttttctag ttcgggaggc
    11041 gccttccccg tgggttcgat tcctaccgtc tcgacgcggc ccttgtcccg ctgcaccgaa
    11101 gcacgcgaaa gcctacgcgc ccgagaccgg cggtgcgtga aacggggccc aaggttggtg
    11161 aacctgggtg aagagtggtg aagcttggtg aagctcggcg atccctcacc aaagccagcc
    11221 cgtccgcgcg cgtgtcgggc cttacttagg gagagcaccc cggcgatcaa agaccggccg
    11281 tttagtctcg atggtcgccc gacaacacag cgacgacgcg tctctcgccg ggggcgacgt
    11341 aggtgctctc tcgataggcg acattgtcgc ccattctcac ctcgaccgta tgcgtgcccg
    11401 acacgacgtc gacgacgtgc gagcggttgc tggcatcgcc ctcgccggtt tgcttcccat
    11461 cgacgagcag cgtcgatccc acgggcacgc cggtgacgat caactgctgc gtgaccggcg
    11521 gcggcgcctt gcggtgaaac cagccgcaac cggtcaaaca aagcagcaat acgagcgagg
    11581 cgagtttcat gattattttt ttgtggtcag ctgcttgagt tcctcgagac cctcggccgc
    11641 gcgttgcgtg atcacctcga atgcctggct gctcgacttg ctgacgagtt ccgccatctc
    11701 acgcatgttg gccacggctt gctcgaacgc cgccttggcg acttcggctt gcttggccgc
    11761 ggcctccttc ggtccgctgc tggcggccac ttgctgcgcg gcgctggcgg cgccttgcat
    11821 cacttgctgc atcatttcca tctggcgctt ggcgagcgcc ttcaatcctt cggcggcgtg
    11881 ctgattcgcc tgcttcaacg cagcgatatt tttctcctgc gcgctcagca gcgactgcat
    11941 atcgacgccg gggattttga aatcgccgag cattttctgc acatcgaatt tactgaagtc
    12001 gaatccggaa aaaggactat tgtcattgct gctcatgacg cgatctcctt gaaaggttag
    12061 ccgcccatcg ccccactgtc ggtatacgcg gtgccccgcc gagcgtcaat cgcacgcacc
    12121 cggcgcagat ctgcaacact actgcgcggt atcgcacgca accacggttt gctgctgctc
    12181 gcccaaaccc gttatgccca atttcaccgt gtcgccgacg ttgagatact tcggcggctt
    12241 ttgcccgaga cccactcccg ccggagtgcc cgtgctgatc acatctcccg gttccagggt
    12301 catgtactgg ctgacgtagg atacgatgaa cacggggttg aagatcatgt ctcgcgtcga
    12361 accggattgc tggcgcttgc cgttgacatc gagccacagc gccagatcgc tggtgtcgac
    12421 ttcgtcggcg gtcacgagcc agggaccgat gggaccgaag ctgtcgcagc ctttgccctt
    12481 gtcccattgc ttgccgcgct ccagctggaa cagtcgttcc gagacatcgt tgcagacgca
    12541 gtagccggca atcgaggcga gcgccgcgcg ttcatcggca taacggcaga cgctgccgat
    12601 caccaatgcg agttcgactt cccaatcgag tttggtcgag ccgcgcggct tgatgatggg
    12661 atcgttcggc ccagagatcg cggaggtggc tttggtgaag acttcgggct cgctcggcac
    12721 cgccacaccg ccttctcgtg cgtgatcggc gtagttcaat ccgatgcaaa tgaatttgtg
    12781 cgggcgcgcg acacaggcgc cgaagcgcgg cgtgccgggg accagcggca gcgcgccgac
    12841 gttgagggct cgcagccgcg ccaagcctgg cggcgacagg gccgccgcgt cgatgtcggg
    12901 tacgcgatcg ccgagatcgc gaatccggcc ctcttgatcc aacacgcccg ggcgctctgc
    12961 gcccactgct ccatatctca cgagtcgcat ggcaacctct ctttaattag cccaaccacc
    13021 gtcgatgaca tgacactgac ccgtgacgaa tgccgactca tctgctgcca ggtagagtgc
    13081 cagcgaggca atctccggcg ccgtcccgag ccgccccatc ggctgccgcg ccgtgaacga
    13141 tttgcgggcg gcctcataat cgccggtggc ccgcaggcgc tcttgcagcg aaggggtgtc
    13201 gaccgtgccc gggcaaatcg cgttgcaacg aacaccccgg gtcacgaagt cggcggcgat
    13261 cgatttcgtg agtccgacca ccgcggcctt ggtcgtgccg tacacgaagc gattcggcac
    13321 gcctttgatc gagccgcaca ccgaggataa gttgatgatt gatccgcggc cgcgagcgag
    13381 catgcccggc agaacggccg aggcgagccg gtacatcgag gcgacgttca agtcgatgga
    13441 aaaagcccag gcctcttcat cgcactcgag gatcgaacct gagtgcacgt aacccgcgca
    13501 gttgaccagc acgtccacat cgccgacgtg ttggatcgcg gcggcgatgg cactcttgtc
    13561 gcgcacatcg agctgcagcg gtcggcaccc ggtgagatcg gcgagcagct cggcgcgaat
    13621 gtcgctggcc cagaccgtcg ctcccgcggc ggcgaactgt tcggcgacgg cacggccaat
    13681 accctgcccg gccgcggtga tgagtgcggt cttgccgctt aaacgtgcgt tttgcatcgg
    13741 tctgctcctc gctaacatca ggcgccggcc gcggcgcccg gacacggcgc atccgggcgc
    13801 agcaggccct ggcttttaag tgcgctccag aattcgacgg gtatcggcgt cgcatacagc
    13861 gcgcgcgtct caccgatttg cgccgcactc acgagccccg gtatcacgct cgccacgctc
    13921 ggatgggcca gcggaaactg caaggcggcg gcggccagcg cgattccgaa cgtcgcgcag
    13981 caagattcca ggcggcgcac ccgatcgatc accgcgggcg gcgcgggacc gtagtcgtaa
    14041 tacaggggcc cgccgtggcg ggtgccggtc gcgagaatcc cggaattata cgggccgccg
    14101 atcaccaagg aaatctggcg ccgctggcat tcgggaagca gatgatccag tgcgggttgc
    14161 tcgagcagcg tgtagcgccc cgcaagcagc acgaggtcta agtccgcgtg atccatcgcc
    14221 cggcgacaga cctcgatctc attgacgccg agaccgaagg ccttgatcgc gccggccgag
    14281 cgcagctcgg cgagcgcgcg aaatccgccg cccgcggtga gttgcgaaaa aaaatgctcg
    14341 ctcatgggcc catgcgtgtg agcgccgatg tcatgcacgt acagcacatc gatcgaggcc
    14401 aacccgaggc gctgcagact cgcctcccat gaattcaaga caccgtcata agtgtagtca
    14461 aagaccgcag cgaacggcat ggacgacacg tagccctgcc gcggcgatgc gtccatgatc
    14521 gacggatcgg gcttgagaag gcgtcccact ttcgtcgaca cggcgtacgc gggatgcccg
    14581 cgcaaggcgt cgcccaagcg ccgctcgctc aatccaaatc catagaaggg cgccgtgtcg
    14641 aaataactga tgccggcctc gagcgccgcc cggcaggtca gccgcgcctg atcatcgtcg
    14701 agcgcgcgat acaggttgcc gagcggagcg ccgcccagcc caatctccga cagacgcagg
    14761 ccccttttca acactcgaga agcgaaactc ctcacacttc acccaaatgt gcgccgagct
    14821 cgaaaataca gcgcatcgac gcccacttct catcgggcgc ggcgtgcgct aggcggcgtt
    14881 ggaagcccgc catgagagat tcccagcgct cgctggcctc gccggtcttg gcgtccgcga
    14941 ccggccgcgg atagtcatcg ctcgtttcca tgatcatgaa caggcgatca tcgcgctgcc
    15001 agatctgcat gtcctcgacg cccaaggagc gaatgtgcgc gatgaccagc ggccagacgg
    15061 cgcccggttc atgcaggcgg caatattccc gaatcaacgc ggcgtcggcc ttgaggtcga
    15121 gtgcaaagca gcggcgctta gacatcgcgc gcccgattgc tcaaagccgg gaaggcgagc
    15181 gcaaagccgg tgcacaccaa gaagcacagc gcggggatgc acatcgcccg atgaataccg
    15241 cccatgtgtt ctgacaacag acccgtgagc ggcgccatga gcgcgccgcc gatgatcgac
    15301 atgatcaaga acgaagcacc caattcggtg tcctcttgca gctcgcgcac gcccaaggcg
    15361 aatatcgttg gaaacataat cgacatgaaa aggcttgtca tccacagcga gccgacggcg
    15421 agccaccccg aagcgcaggc cgcgaccaaa cacaacacga cattcgccgc gccataccag
    15481 gccagcatac gctccggccg cactcgcgct tgaatgaagg cgccgctaaa gcggccgagc
    15541 atcagcattc ctaagctcgc cgacagcaag aatgccgccc gcttctccgg caagttcggc
    15601 agcagcgcct tgctgaagtc gatgaaaaaa ctccacaccg tgacttgcgc gccgacgtag
    15661 aaaaactgcg ccacgacggc gtagcgcagt accgggttgc gcagaacgcc ccagagcgaa
    15721 cgcggcgatg acctgcgcgc agcggacttt tcgagcgcca gctgcgggaa gggcgtccag
    15781 gcgacggcga gcgccaacag cacgatgccg atgcccatcg tcacataggg cagttgcacc
    15841 gcgtgagatt ccgatgcccg ataagcgccg agttccatgg gggtcatcgc gcccagctga
    15901 gccgcgctcg gttcgattcc tgaaaagatg aagagtccgc cgatgatggg gccgaagatc
    15961 gcaccgagtc cgtagaacga ctgggcgaga ttcaatcggg cggcggccgt gctcggcttg
    16021 cccaagatcg atatgtaggg gttcgccgcg gtttcgagga aggcgaggcc gaaggcgatg
    16081 atatagagcg ccgccaagaa ccagccgaag cgctgcatgt tcgcggccgg ataaaacaga
    16141 aacgcgccgg ccgcatatag gctgagtccc aagatgatgg tcggcttgta gccgacgcga
    16201 cggatgagga gtccggcggg gatcgccgcg cagaaatagc cgacgtagaa agccagctgt
    16261 atcacgctcg attcagctcg cgacagcgcc agcgcctttt gaaattgacg aatcaggacg
    16321 tcattgagcg cggccgcgaa ggcccaggcg aagaataggc tcgtcaccag gacgaagggc
    16381 cgcagatatt gccgatcgac gatcgcgtcg cgggcattcg gcattcctgg tccagcgtcg
    16441 gtcatgccct gctcctgtcg cgcgctcgag tatacctata aaatgcgttt tcacccctca
    16501 aggtacatgg agtcgacccg tgggcgctaa ccgtggtctc aagcacgcgc tattgatcat
    16561 tgcgatcgca gtgatcgcag cgggtatgca cgcgcgcgtg ctcgcggcgc cggccgcggc
    16621 cgatgcgccg tcctatacgc ccgcgcccag caatcttgcg gcgcgtgact ggttccagga
    16681 cgcgaaattc ggaatctttt tgcactgggg tctgtatagc gaacttggcg gcgtgggctc
    16741 gcagagccta tccgaatgga tcatgaatga ggcgcgcatc ccggcggcga aatacgagcg
    16801 cctggcgctg ttcttcaatc cgcggagctt cgatgccgac acctgggtgc ggcagttcaa
    16861 ggatgccggt gcccgctaca tcgtcatcac ctcgaaacac cacgatggtt tcgccatgtt
    16921 tcacagcgca gtgtcgccct acaacgtggt cgacgccacg ccgttcaagc gcgatccgct
    16981 tgccgaactc gcggcggcct gtcggcggca gaacatcaag ctgttcttct attactcgca
    17041 gctcgattgg cacaacccgg actatttccc gcgcggtagg accgggcagt ctgccgggcg
    17101 gccggcggcg ggcgactgga gtcgttacct cgactatcaa aacgcgcaga tcaaggagct
    17161 ggtgacgaat tacgggccca tcggcggcat ctggttcgac ggctggtggg atcaggaggg
    17221 cacggcgctg caaaattcct gggatcttgg ccgcacctac cgggtgattc acgacgcgca
    17281 gccggccgcg ctgatcgtca acaatcatca ccaaacgccg tttcccggag aggactatca
    17341 gaccttcgag cgcgacctgc cgggcgagaa cggcatgggc ttcaaccgcg cgcaggtcgc
    17401 tcagctgccg ttggaaatgt ccgaaacgat gaatggcacg tggggcttta gcctcaccga
    17461 tgcgaattac aaatccacac agaccttgat tcgcacgctg gtgggggccg ccggtcgggg
    17521 cgccaatctg ttgttgaaca cgggtccgat gccgaatgga cagatccagg gtgagaactt
    17581 gcagacgctc gccgagatcg gcacgtggtt gcgggcgaat ggatcgagca tctacggcac
    17641 tcgcgcaggt ccgatcgcgc cgcggccatg gggagtgacc accgaatcga aaggccagat
    17701 ctacgtgcat atcctcgatt ggcaggacga cacgctgttt ctcccgctgc gtcgcccggt
    17761 caagagcgcc gctctgatta cgggcgcgtc catccccgtc cgccgggttc acgacgggat
    17821 cgaactgaca ctgcccgcgg cccgcgcgga tgactgggat cggatcgtgg tgctcgagaa
    17881 ataggcctcg tttgatcgac gcgcatcagc attattggca actcgaacgc ggcgattacg
    17941 gctggttgac gcccgatctc accgggctgt atcgcgactt cttaccggcg cagttgcagg
    18001 gcacgctcgc cgactgcggc gtgaccggta cgctggccgt gcaggcggcg ccgaccgaag
    18061 cggagacgcg gtttttgttc gacctcgccc gccggcacgc ctcgatcctc ggcatcgtcg
    18121 gctgggtgga tttcgaggcg cacgacgtgg cgcgacggat cgacgcgctg atggcggagg
    18181 gtggcgggct tttgataggg ttgcggccga tggttcaaga catcgcggat cccgcctggt
    18241 tggatcgcga ctcgctcgat cgcgcctttg gcgtactcac ggaacgcgaa ttggtgttcg
    18301 atgcgctggt gacgcccgtg cacttgggct ttctcgagcg gcgcttacgg cgccatcccg
    18361 agcttcgctg cgtcatcgat catgccggca aaccaacgcc gggcgctcgc tcgctcgccg
    18421 cttggtcggc gcgcttgcgg agtctcgcga accacacctc ggcgtattgc aagctgtccg
    18481 ggttgctcac gcaattcgag gcgccggtga cgctcgatgt cctcgcgccg tacgtgtcgc
    18541 gagtgttcga ctacttcggg cccgagcgca tcatctgggg cagcgactgg ccggtgctga
    18601 cgcttcgagc cgcgtaccgc gattggctcg atctgtcgtt ggagctgatc cgaatgcatg
    18661 cgccgcaggc aggcgcggcc atcttcgcgg acaatgcccg gcgcgcgtat cgcttggact
    18721 aatgggaatt ctcgaatgat cgtcatcacc gcactcgaga ctcacgacat ccgcttccca
    18781 acgtccaaag agctggatgg ctcggacgcc atgaacgccg atcccgatta ttccgcggcg
    18841 tacgtggtgc tcaagaccaa ctgccccgac gggctctgcg gccacgggtt tgtcttcacc
    18901 atcggccgcg gcaacgatat ccagacgcgc gcctgcgcgg cgcttgacta cctcgtggtg
    18961 ggacggcgcg tcgaggaggt cgtgtcggat ctcggggcct tcgcccggag tctcaccggc
    19021 gattcgcagc tacgctggtt gggcccggaa aaaggcgtca tg
//
LOCUS       DASWZT010000460         2677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_47285, whole genome shotgun sequence.
ACCESSION   DASWZT010000460 DASWZT010000000
VERSION     DASWZT010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47285"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VGE92_13185"
     CDS             complement(<1..582)
                     /locus_tag="VGE92_13185"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY1314828.1"
                     /translation="MNTFTFLQKAAMLRPDQAALVHGAESISYREFHDRALAIGGNLL
                     ALGLAPGDRVAFCLANSPRILETIYGCFAAGLIVVPINARLHPREMAYIVSNSEARAL
                     IHGPEYQAGITEHIAEFAGLEHRICTTAVDGALAFENLLNATFALAQSAEVSATDPCW
                     LFYTSGTTGKPKGAIWTHRTVRVVIMNYLADVHN"
     gene            complement(593..994)
                     /locus_tag="VGE92_13190"
     CDS             complement(593..994)
                     /locus_tag="VGE92_13190"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEY1314829.1"
                     /translation="MSNPIRDEFRAGLARGDLLIQKCNDCGKLNMYPRHACPFCQSLS
                     LGWHKSTGRGVLHSFTVLRAGAPEGFEGDLPYAVGVIKLDDGVQLLGRLLPDADGDWQ
                     VYSCDARVQFKAAAPESVARRPCAWFERSAV"
     gene            complement(991..2217)
                     /locus_tag="VGE92_13195"
     CDS             complement(991..2217)
                     /locus_tag="VGE92_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEY1314830.1"
                     /translation="MADTWPLNIDANSPNDPVFIGVGELPSGRYPERNFIGDLTHVGL
                     RALADARMVPRDVDTILLIPCLHSPFDQADLIFSRMAEEFGLLGSLKASFMVHSGGST
                     SDNAVRLASGLIASGHARTVLVLHCERWGSADVNAMVTMLSKYGIPNEWEAPTGLQFN
                     AIGALITRRYMHATQSSAADMASFCVTLRKWAQLNPNAMYRGKPLTVEKVLASKVVAE
                     PLHALECPMLADGAVAFVMTSAENAKARGLDTVRVAGSGGSVSHYSIGQEPDLAALGW
                     ATAARRAYEQSGWGPKDAQIAEIYDTYAAVTTIAVEGLGFCALGEGARWFASGAADPG
                     GPLPCNTNGGMLSAGHTGVGGGTALLVEGVRQLLGRAGSERQIEGVRRCIVGGTGGSY
                     MDAQVLLLERRTRGRA"
     gene            complement(2219..>2677)
                     /locus_tag="VGE92_13200"
     CDS             complement(2219..>2677)
                     /locus_tag="VGE92_13200"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEY1314831.1"
                     /translation="PQDKAFEDFARGDIVITRGRTVDIGDISTFSGLTGDHYPLHTDE
                     EFGKATRFGTRIAHGPLTFALAVGLVGMSGFYGNAITALVEVKSLRALKPVIPGDTLK
                     VHAEVVELSEARNPKYGTVHMKYSVRNQREEEVMDFLQIMLARRRTPQGG"
BASE COUNT          515 a          871 c          828 g          463 t
ORIGIN      
        1 gttgtgtacg tcggccaggt aattcatgat cacgacgcgc accgtgcggt gcgtccagat
       61 cgcgcccttg ggtttgccgg tagtgcctga ggtatagaac agccagcacg gatcggtcgc
      121 tgagacttcc gccgattgcg ccaacgcaaa agttgcattc aacagattct cgaaggcgag
      181 cgccccatcg accgccgtcg tgcaaatgcg gtgctcgagt cccgcgaact cggcgatatg
      241 ctccgtgatg cccgcctgat actccgggcc gtggatcagc gccctggcct cggagttact
      301 cacgatgtaa gccatctcgc gcggatgcag gcgagcgttg atcggaacca cgatcagtcc
      361 ggccgcgaag cagccataga tggtttcaag aattcgcgga ctgttggcca agcagaaggc
      421 gacgcggtcg cccggcgcca gcccgagagc caagagattg ccgccgatgg cgagcgcacg
      481 atcatggaat tcacggtagg aaatcgattc cgcgccatgc accaacgcgg cctggtccgg
      541 gcgcaacatc gcggcctttt gcaggaacgt aaaagtgttc atggtcggga catcaaacgg
      601 ccgaacgttc gaaccaggcg cagggacgtc gcgccaccga ttcgggggcg gccgctttga
      661 actgcacacg cgcgtcgcaa ctgtaaacct gccaatcgcc gtccgcgtca ggcagcaggc
      721 ggccgagcag ctgcacgccg tcgtcgagct tgatcacccc cacggcgtag ggcaaatcgc
      781 cctcgaaacc ttccggcgcc ccggcgcgca gcaccgtgaa gctgtgcagc actccgcgtc
      841 ccgtcgactt gtgccacccg agcgagagtg attgacagaa agggcaggca tggcgcggat
      901 acatattgag cttgccgcag tcattgcatt tttgtatgag cagatcgccg cgagccaggc
      961 cggcgcgaaa ctcatcacgt atcgggttgc tcatgcgcgc cccctggtgc gccgctccaa
     1021 cagcaacact tgtgcatcca tgtagctacc gccggtgccg ccgacgatgc aacgccgtac
     1081 gccctcgatc tgacgctcgg aaccggcgcg ccccaacaac tggcgcaccc cctcgaccag
     1141 caacgccgtg ccgccgccga cgccggtgtg gccggccgag agcatgccgc cgttggtatt
     1201 gcagggcagt gggccgccgg ggtctgctgc cccggatgcg aaccagcgag caccctcacc
     1261 caaggcgcag aaacccagtc cctcgacggc gatggtcgtc acggccgcat aggtatcgta
     1321 gatctcggcg atttgcgcgt ccttcggacc ccagcccgat tgctcataag cgcgccgggc
     1381 agcggtggcc cagccgagcg ccgccaaatc tggctcctgg ccgatgctgt agtggctgac
     1441 actgccgccc gagcccgcga cccgcacggt atcgaggccg cgcgccttgg cgttctcggc
     1501 gctcgtcatg acgaatgcca cggcaccgtc ggcgagcatc ggacattcga gcgcatgcaa
     1561 gggttcggct accacttttg atgccaagac cttttccaca gtcagaggct tgccccgata
     1621 catggcgttc ggattgagct gcgcccattt gcgcagcgtc acgcagaaag aagccatatc
     1681 ggcggcgctc gactgggtgg cgtgcatgta gcgccgggtg atgagagccc cgatggcgtt
     1741 gaactgcaag ccggtgggcg cttcccactc gttgggaatg ccgtacttgc tcaacatcgt
     1801 caccatggcg ttcacatcgg ccgaacccca gcgttcgcag tgcaggacca acacggtacg
     1861 ggcgtgaccg gatgcaatca ggccggaggc gagccgcacc gcgttgtcgc tggtcgagcc
     1921 gcccgaatgc accatgaagc tcgccttcag agacccaagc agcccgaact cctcggccat
     1981 tcgcgagaaa atcagatcgg cctgatcgaa cggcgaatgc aggcaaggta tcagcaggat
     2041 ggtgtcgacg tcgcgaggca ccatgcgcgc gtcagcgagc gcgcgcaggc caacatgggt
     2101 cagatcgccg atgaaattac gctcaggata gcggccggac ggcaattcgc cgacgccgat
     2161 gaacaccgga tcgttgggcg aattcgcatc gatgttgaga ggccaggtgt ccgccatttc
     2221 agccgccctg cggcgtgcgc cgtcgtgcca acatgatctg cagaaaatcc atcacctctt
     2281 cctcgcgttg attgcgcacc gaatatttca tatgcacggt gccgtatttc ggattgcggg
     2341 cctccgagag ttcgaccacc tcggcatgga ccttcaaggt atcgcccgga atcacgggct
     2401 tgagcgcgcg caggctcttc acctccacca gcgcggtgat tgcgttgccg taaaatccgc
     2461 tcatcccgac caggccgacg gcgagagcga acgtcaatgg accgtgcgcg attctcgtgc
     2521 cgaagcgcgt ggccttgccg aactcctcgt cggtgtgcag cggatagtga tcccccgtca
     2581 gtccggaaaa cgtcgagatg tccccgatgt cgaccgtgcg cccgcgggtg atgacgatgt
     2641 cgccgcgcgc gaagtcctca aaggccttgt cttgcgg
//
LOCUS       DASWZT010000461         1845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_61685, whole genome shotgun sequence.
ACCESSION   DASWZT010000461 DASWZT010000000
VERSION     DASWZT010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61685"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VGE92_13205"
     CDS             complement(<1..205)
                     /locus_tag="VGE92_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314832.1"
                     /translation="MTDEALQRALALEMTWLSPSELEALHLSLSVATRSVLFSLPLAV
                     AVAWLLTRRRFLGRTLLDAFVHLP"
     gene            247..1284
                     /gene="hemH"
                     /locus_tag="VGE92_13210"
     CDS             247..1284
                     /gene="hemH"
                     /locus_tag="VGE92_13210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY1314833.1"
                     /translation="MSYYRAVAQAEAAPDRIGVLVVNLGTPDSPSYFAVQRYLREFLG
                     DRRVIDTSRLIWLPVLFGIVLPFRPIRTVRNYRKIWMRGGSPLAVYSKRLADKVGVVL
                     REKLGDRVRLELAMTYGKPGIAHAIQSLAEQNVKNLLVLPLYPQYCSSTTGSVFDRVT
                     RVLQHWRWLPETRFINDYFDDAGYIDALAASIREHWAQAGERSHLLLSYHGIPAVYVA
                     EGDPYQMQAETTTRLLVSRLGLAAEDYSHCYQSRFGSVVWLQPYTEDTLRALLKRGVR
                     KLTVVSPSFAVDCLETLQEVAIEYRDKFLEMGGERLTLVPALNDGDRHAQVLAALVSQ
                     QLRGWTDREAT"
     gene            complement(1293..>1845)
                     /locus_tag="VGE92_13215"
     CDS             complement(1293..>1845)
                     /locus_tag="VGE92_13215"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY1314834.1"
                     /translation="DSEIALRRALTSAAKFWVCKRGCELAVEAMEVLGGNGYTEELPL
                     ARIVREMPVNSIWEGSGNIMCLDLIRAFGKSVAAQDAVLHELRQARGMHRDFDAAFER
                     FAATLGPAPLPEASARGFAQNFVTLMQGALLLRAAAGPGAGAVADGFCATRLCAAAGW
                     GAVFGASGTARGTAAILDRAWYE"
BASE COUNT          331 a          582 c          590 g          342 t
ORIGIN      
        1 agggtagatg gacgaaggca tcgagcaagg tacggccgag aaatcggcgc cgcgtgagga
       61 gccaggcaac ggcgaccgcg agcggcaggc tgaacagaac gctgcgcgtc gccacgctga
      121 ggctcaaatg cagcgcctcg agttcgctgg ggctcaacca cgtcatttca agggcaaggg
      181 cacgctgcag ggcttcatcc gtcacgcagt atactgtgcc ggctgaccgg cttaaggatt
      241 tgctcaatgt cgtattatcg cgccgtggcg caagcggagg cggcgcccga ccgtatcggt
      301 gtgctggtgg tcaacctggg cacgccggat tcgccgtcct acttcgccgt gcagcgctat
      361 ttgcgcgaat ttctgggcga tcgccgggta atcgacactt cgcgcctgat ttggctgccc
      421 gtgctgttcg gcatcgtgct gccgtttcgg ccgatcagga ccgtgcgcaa ctatcgcaag
      481 atctggatgc gaggcggctc gccgttggcg gtgtactcga agcgcctcgc ggacaaggtc
      541 ggtgtggtgt tgcgcgaaaa gttgggcgat cgagtgcggc tcgagctcgc gatgacctac
      601 ggcaagccgg gcatcgccca cgcaattcaa tctttggccg agcaaaacgt caaaaatctc
      661 ctggtgctgc ccctatatcc gcaatattgc tcttcgacga ccggctcggt gttcgaccgg
      721 gtcacccgcg tcttgcagca ttggcgttgg ctcccggaaa cccgcttcat caacgactat
      781 ttcgatgatg cgggctacat cgacgcactt gcggccagca ttcgcgagca ttgggcgcag
      841 gcgggcgagc gctcgcatct gttgctctcg tatcacggca ttcctgccgt atacgttgcc
      901 gaaggcgacc cctatcagat gcaagccgaa acgaccaccc gcctgctggt gtcgcggtta
      961 gggttggctg cggaagacta ttcgcattgc tatcaatcgc gttttggctc ggtggtgtgg
     1021 ctgcagccct ataccgagga cacgctgcgg gcgttgctca aacgcggcgt gcgcaagctc
     1081 accgtcgtgt cgccgtcatt cgccgtcgat tgcttggaga cgctccagga ggtcgcgatc
     1141 gagtaccgcg acaaattcct cgagatgggc ggcgagagat tgaccttggt gcccgccttg
     1201 aacgatggcg accggcatgc gcaggtgctg gcggcgctcg tgagccagca gctgcgcggt
     1261 tggacggacc gagaggcgac atgagcaacc ggctattcgt accaggcacg atccaggatc
     1321 gctgcggtgc cccgtgccgt accacttgct ccaaacaccg caccccagcc ggcagcggcg
     1381 cacaaacggg tggcgcagaa gccatccgcc acggcgcccg cgcccgggcc cgcagccgct
     1441 cgcagtaaaa gtgcaccctg catcagagtc acgaaatttt gcgcaaagcc gcgggcacta
     1501 gcctccggca gcggggcggg gccgagcgtc gctgcgaatc gttcgaatgc ggcatcgaaa
     1561 tcgcgatgca tgcctcgcgc ctgtcgaagt tcgtgcagca ccgcgtcctg ggcggcgacg
     1621 gattttccga aggcgcggat gaggtcgagg cacatgatgt tgcccgaccc ctcccagatc
     1681 gaattcaccg gcatctcgcg cacgatgcgc gcgagcggca gctcctcggt gtagccgttg
     1741 ccgcccaata cttccatggc ttccactgcc agctcgcagc cgcgcttgca gacccagaat
     1801 ttcgccgccg aagtgagcgc gcgccgcagc gcgatctcgc tgtcc
//
LOCUS       DASWZT010000462        10040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_8479, whole genome shotgun sequence.
ACCESSION   DASWZT010000462 DASWZT010000000
VERSION     DASWZT010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10040
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8479"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VGE92_13220"
     CDS             complement(<1..809)
                     /locus_tag="VGE92_13220"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY1314835.1"
                     /translation="MNALAYDYVIVGAGSAGCVLANRLSASADVRVLLLEAGGADWSP
                     FIHMPAGIARLVDDQRVDWRYHTEPEPALNGRRLYWPRGRVLGGSSSINAMCYTRGHP
                     FDYDEWAASAGQGWSYAGVLPYFLRSEDQAHGAGEYHGAGGPLSVTDLKYRNPLSSVF
                     VEAGVACGLARNADFNGVEQAGVGFYQVTQRDGRRCSAAVAFLRPARARANLKIRTHC
                     LATRVIFSGLRASAVEYLQHGVLQVARAHREVLLAAGAVGSPQLLLLSGLGP"
     gene            complement(907..2124)
                     /locus_tag="VGE92_13225"
     CDS             complement(907..2124)
                     /locus_tag="VGE92_13225"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY1314836.1"
                     /translation="MLTIEPARRRRAGCGKVAIALAGGGPLGAFYELGALHALEEAIV
                     GRELTNFDMYVGVSSGSVVAAGLANGIDTTAMGSIFIHDSSTVLPFTPEMLMRPAFGE
                     YGRRLARLPEALVEIGRLFARDPLHGVWPTMVRSLDKVIPTALFDNGPLERYLHALFA
                     GNGRTDDFRKLKADLLLVATNLNTGASVAFGAAHHDRVPISRAVAASAALPGLYAPVQ
                     IDAQHYVDGALIRTMHASLALEAGCELVICINPLVPFDASRAPVRRRANLADAGLPDI
                     LGQTFRALIHSRMQVGMASYGARFPNADTLLLEPDRGDERLFFANVFRYSGRQSLVDH
                     AYQRTRRDLRAQADALGAILDRHGLALDMKRLRERHRTFSTATRERATRSRQVVQRLG
                     RALGRLERALSSQ"
     gene            complement(2127..2618)
                     /locus_tag="VGE92_13230"
     CDS             complement(2127..2618)
                     /locus_tag="VGE92_13230"
                     /inference="COORDINATES: protein motif:HMM:NF017415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEY1314837.1"
                     /translation="MATKRKSRKSARKTKSTAPERVLDTMNQIWLAGLGAMAKAQQGA
                     PRMLHDLAAEGARIQANTRGSAEKALGGLLNDVKATIDSGVSQVRGQAGDALDNLETI
                     FQTRVRRALTQLGVPSSDEVASLSKRVDQLNVNINKLARKPASARRSPATRKAGTSHT
                     TAA"
     gene            2743..3603
                     /locus_tag="VGE92_13235"
     CDS             2743..3603
                     /locus_tag="VGE92_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314838.1"
                     /translation="MRTESKSVRTAGSSTFEYPDDRLHRLWETLHHGDREPWPEAKSI
                     ARLGKSGPFAEWLDAHNGGPAAVAQELRDAWRAFHAGDFVKAIKIGSALGPLGAVAAN
                     KAAAIYAYYGRRSAAQRLKLLESAATRGEAAVKLLPDYSNAHYMLALALGRYSQDISI
                     IKAATTGVAGRVRTHLERALELEPSHAEGHVALGLYHAELIGKLGKIAAALTYGASPD
                     DALEQFRRALKLAPTSPIVHIEYANGLLLLDAKRHRAQAEELYGQAAAHEPIDAMEQF
                     DVARAQRGLA"
     gene            complement(3548..5014)
                     /locus_tag="VGE92_13240"
     CDS             complement(3548..5014)
                     /locus_tag="VGE92_13240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEY1314839.1"
                     /translation="MSAVENMSSVDRAWLLMDRPSHPMMIVGLLVLDARIERAALRKL
                     IERRFLAFSRFRSVPANGRIAAAWQESAQFDLDDHVLSCALPLPAGQQELQALVGELA
                     STPFNPGRPPWMFHLVENFDGRSAVIVRIHHAYADGVALVQVLLSLADGERKGNHSNS
                     STVHSAAASRTDSSSGWLPDRVLEILQGGANLTGMAVHYTLHPRETSTLARQALGMAA
                     ELAHLGLLADDPATPLKRPMSGIRRAAWTDALPLQEVRTISHVLSCTVNDVLMSTLAG
                     ALGRYLESRGYPVPGVTIRAAVPVNLRGAGDPQPSMGNRFGLVFVDLPIGIRHPLMRL
                     YAVRATIQNLKHSPQALITLGLLSLIGSLPATAAEPMIALFSSKASVVASNLPGPSRP
                     LYLAGAAVSQLLFWVPQAGDIGIGVSMLSYDDRVQFGVIADRYLIPEPGDLVAEIGVE
                     FERLVLLVLLGGAAVAPAPPVKRDRAAPSRRRTAPSHR"
     gene            5126..7108
                     /locus_tag="VGE92_13245"
     CDS             5126..7108
                     /locus_tag="VGE92_13245"
                     /inference="COORDINATES: protein motif:HMM:NF005539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY1314840.1"
                     /translation="MAYFVTGGTGFIGRHFIAEISVHGEPIYVLVRPGSRTRFERLVK
                     DNGVRGSLIIAVEGDLSDELLGVPASMREQLRGHIGHFVHLGALYDLAAEAAPLERSN
                     VAGTRNALQLAHDLKAGCFHQVSSIAAAGRYEGIFTESMFDQAQDLEHPYFRTKHESE
                     ALVRSSCRIPWRIYRPGMVVGHSVSGAMDKVDGPYYLFKLIQKVRDTVPSWLPLVGFE
                     GGHINLVPVDFVAAALAHLMHAPGQDGQCFHLTDPQDRRIGDVLNLFAKAAHAPTMTL
                     RLDSDLLQRLASAVGAVSELGHPLRRLKVRVLREFGIPAPVVSLLDYPTRFDASRAQE
                     LLAAAGIQVPCLENYAWRLWDYWERQLDSDLDTVGNLRAAAGGKTILITGGSSGIGRA
                     SAVKLADAGAHVLIVGRNVENLTRVSSEIERRGGRVSQYACDLTDAAASANLLATLLA
                     EHGHIDILINNAGRSIRRSIDNSYDRMHDYERLMSINYFAAVRMTLGLLPAMVKHGSG
                     HVICISSIGVITNAARFAAYNASKAALEAFTRCAAAEYSDRNICFTVINMPLVRTPMI
                     APTKLYERFHVLQPEQAADQVCAAVVHRPARLATRLGTLAQVVEAFAPRINTAVMSEG
                     FTMFPESEAAGGSSPAARPAEASAVAFAALMRGAHW"
     gene            complement(7115..8788)
                     /locus_tag="VGE92_13250"
     CDS             complement(7115..8788)
                     /locus_tag="VGE92_13250"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY1314841.1"
                     /translation="MDKIWLKAYPHGIAAEIDADSHPSLVHLLEESCRRFAERPAFHN
                     LGVTLSYRDLERQSRYFGAYLLALGLQPGDRVALMMPNVLHYPIAMFGALRAGLTVVN
                     VNPLYTPPELRHQLRDSGARAIVILENFAHLLAQVRAATQIQHIVIASLGDLAPFPKR
                     SVVNFLVRRVKRLVPAYDLPDARPFRSALKRGARGKWHPPVIGPADVAFLQYTGGTTG
                     VAKGAVLTHRNMVANLLQVGEFWKNVIEPGREVIITPLPLYHIFCLTCNCLVFMRYGG
                     LNVLITNPRDIGAFVHELRSWKFSIITGVNTLYSALLGHPSFAKLDFSHLKLGAAGGM
                     ALHPSVAERWRALTGRPLAEGYGLTESSPVVSCNSYEAPQLGTVGLPLPSTEISIRND
                     EVEVPLGDPGELCVRGPQVMRGYWNRAEDTAKTLTPDGWLRTGDIAVMAADGLLRIVD
                     RKKDLIIVSGFKVFPNEIEAAVGEHPAVLECGCIGVPDAHSGEAVKIFVVLREGCSLT
                     PEDLREHSRQRLTGYKIPKHVEFRSILPKTNVGKILRRELAREEAVRAA"
     gene            9254..9685
                     /locus_tag="VGE92_13255"
     CDS             9254..9685
                     /locus_tag="VGE92_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314842.1"
                     /translation="MDFYYVKLWTVGAAGESTESSGALMVPTGAPPACSGARPIVLYA
                     HGTNSDKTLNIADVTNATNTEGVLIAAMFAAQGYIVVAPNYAGYDISRLGYPPYLNAE
                     QQSGEMLDMLTAARAALPKTLSAATSGEGAGAAAAPGVLQE"
     gene            9753..10037
                     /locus_tag="VGE92_13260"
     CDS             9753..10037
                     /locus_tag="VGE92_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314843.1"
                     /translation="MFFPVNTGVMAEFWSPLVQAGLITVLDVNGTPSGPFAALQTGFQ
                     ESQAAQLAYYASAAGGRLMPAAAQQLIVEGYHTSVAPFCSAAARAFFGQL"
BASE COUNT         1863 a         3183 c         3175 g         1819 t
ORIGIN      
        1 ggcccgaggc cggagagcag cagcagttgg ggagaaccga ccgcgcctgc cgcgagtaag
       61 acttcgcgat gcgcccgggc gacttgcagg actccatgct gtaagtattc gacggcgctg
      121 gcacgcaatc cagaaaaaat gacgcgggtg gctagacagt gtgttcgtat cttgaggttc
      181 gctcgtgcgc gcgctggacg gaggaaggcg actgcagcgc tgcagcgccg tccgtcccgt
      241 tgcgtcactt gataaaaccc tacgcctgcc tgttcaacgc cgttgaaatc agcattgcgc
      301 gcaagaccac aggcaacccc tgcctcgacg aacacgctgc tcaacggatt tcgatacttc
      361 aaatcggtga cggacaacgg tccgccggcg ccgtgatact cgccggcgcc atgggcctgg
      421 tcctcggatc gcaaaaagta gggcagcacg ccggcataac tccatccctg tcccgccgac
      481 gcggcccatt cgtcatagtc gaagggatgc ccgcgcgtgt agcacatcgc attgatcgag
      541 ctgctgccgc cgagcacccg cccacgcggc caataaagcc ggcggccgtt gagagcgggc
      601 tcgggctcgg tgtggtaacg ccaatccacg cgttgatcgt ctaccagtcg cgcgatgccg
      661 gcgggcatat gaatgaacgg actccagtcg gcgccgccgg cttcgagcag cagcacacga
      721 acatcggcac tcgccgagag gcgattggcc agtacgcagc cggccgaacc agctccgact
      781 atcacgtagt cgtatgctag tgcgttcatt cggcgcccgc ccgctcgccg aacaaggcat
      841 gcatcagccg cgtctcccga gtgtcggcca cccccggcgt acggccatgc gtggtcggtg
      901 ggacattcat tgcgacgaca atgctcgctc gagccgaccg agggcgcgtc cgagacgttg
      961 gaccacctgc cgggaacgcg tggcgcgttc ccgcgtcgcc gtggagaaag tgcgatgccg
     1021 ttcgcgcagc cgcttcatat ccagagcgag cccgtgtctg tccaatatgg cgcccaacgc
     1081 atcggcctgg gcccgcaaat cgcgccgggt gcgctgatag gcgtgatcca cgaggctctg
     1141 ccgacctgaa tatcggaaca cgttggcaaa gaacagcctc tcgtcgccgc ggtccggttc
     1201 gagcagaagc gtatcggcgt tcggaaatcg cgcgccatag ctcgccatgc cgacttgcat
     1261 gcgcgaatga atcagtgcgc gaaaggtctg tcctagaatg tccggcagtc ccgcgtcggc
     1321 aagattcgca cgccgccgta caggcgcacg cgaggcgtcg aacggcacca gcggattgat
     1381 gcaaatgacg agctcgcagc ccgcttcgag ggcgagcgag gcatgcatgg tgcggatgag
     1441 ggcgccatcc acatagtgct gcgcatcgat ttggacgggg gcatacagtc ccgggagcgc
     1501 cgcgctcgcg gcgacggctc gcgagatcgg tactcgatcg tgatgtgcgg cgccaaaggc
     1561 cacggatgcg ccggtgttga gattcgtcgc caccaacaag agatccgcct ttagcttgcg
     1621 aaagtcatcg gtgcggccgt tgccggcgaa cagggcgtgc agatagcgct ccaacgggcc
     1681 gttgtcgaag agcgccgtgg gaatcacctt gtccagtgag cggaccatcg tcggccatac
     1741 gccgtgcaac gggtcgcggg cgaacaatcg gccgatctca acgagggcct ccggcagtcg
     1801 cgcgaggcgc cggccgtact cgccgaaggc gggacgcatg agcatttccg gcgtgaacgg
     1861 cagcaccgtg gagctgtcat gaatgaaaat cgagcccatc gcggtggtgt cgatgccgtt
     1921 ggcgaggcct gccgccacga ccgaccccga actcacgccg acatacatgt cgaagttggt
     1981 cagctcgcgg cccacgatgg cctcttcgag cgcgtgcagt gcgcccaact cgtaaaatgc
     2041 cccgagcggc ccaccgcctg ccagcgcgat ggccactttg ccgcagccag cgcggcggcg
     2101 ccgcgctggc tcgatggtca gcacttttat gctgcggttg tgtgcgaggt gccggccttg
     2161 cgcgtcgccg gcgagcgacg cgcagacgcg ggtttgcgcg cgagtttgtt gatattgaca
     2221 ttgagctgat ccacgcgctt gctcaagctc gcgacctcgt ccgagctcgg cacaccgagc
     2281 tgggtcagcg cccggcgcac gcgcgtctgg aaaattgtct ccagattgtc cagggcatct
     2341 cccgcctgtc ctcgcacctg actcactccg gaatcgatgg tcgccttcac gtcgttcagc
     2401 aaaccgccga gcgccttctc tgcggagcct cgggtgttcg cttggatgcg tgcgccttcg
     2461 gcggccaggt cgtgcagcat ccgcggggca ccctgctggg cttttgccat cgcaccgagt
     2521 ccggccaacc agatttgatt catcgtatcg agcacccgct ccggcgccgt gctcttggtc
     2581 ttgcgggcgc tcttgcgcga ttttcgcttc gttgccatga cattgtcccc tctaagatgt
     2641 gtagaaaccc ttatcggctc ggccgtgaaa tcagcctacg atgcaatcct atcgagttgg
     2701 ttggggcatg aggattggta cttgccgcag aggagtaggg aaatgcgcac ggaaagcaaa
     2761 tcggttcgca ccgcgggcag cagcacattc gagtatccgg acgacaggtt acacaggctc
     2821 tgggagacac tgcaccatgg cgatcgcgag ccgtggcccg aggccaagtc aatcgcgcgc
     2881 ctcggcaagt ccgggccgtt cgccgagtgg cttgatgcgc acaacggcgg accggcggcc
     2941 gttgcgcagg agctgcgaga tgcttggcgc gcgttccacg cgggagattt cgtcaaggcc
     3001 atcaagatcg gcagcgcgct gggtccgttg ggagcggtcg ccgcgaacaa ggccgccgcg
     3061 atttacgcct actatgggcg gcgcagcgcc gcgcagcggc tcaagttgct cgaaagcgcc
     3121 gctacgcgcg gcgaggcggc ggtcaaactg ctgcccgatt attcgaatgc gcactacatg
     3181 ctcgcactcg cattgggtcg ctatagccag gacatctcca tcatcaaggc ggcgacgacg
     3241 ggtgtggcgg gccgcgtccg cactcacctc gaacgcgcgc tcgaactcga gccctcgcat
     3301 gccgaaggac acgtcgcact cggtctatat catgccgaac tcattgggaa actgggcaag
     3361 atcgcggcgg ccctgacata cggtgcctcc ccggacgacg ccctcgagca atttcggcgc
     3421 gccctcaagc tcgcccctac ctcgcccatc gtgcacatcg aatacgccaa cggtttgctg
     3481 ttgctggacg ccaagcgcca ccgcgcgcag gcggaggaat tgtacggcca ggcggcggcg
     3541 cacgagccta tcgatgcgat ggagcagttc gacgtcgcga gggcgcagcg cggtctcgct
     3601 tgacgggagg tgccggcgcc actgcggcgc cgccgaggag gaccagcaat accaggcgtt
     3661 cgaactcgac tccgatctct gcgacgagat cccccggctc ggggatcagg taccggtcgg
     3721 cgatcacgcc gaattgcacc cggtcatcgt aactcagcat gctgacgccg atgccgatat
     3781 caccggcctg cggcacccag aataacagtt gcgaaaccgc cgctcctgcg agataaagcg
     3841 gcctcgacgg ccccggcaaa tttgatgcca ccacgctcgc cttggagctg aataaggcga
     3901 tcatcggttc cgcagccgtc gcggggaggc tgccgatcag cgacagcaat ccgagcgtga
     3961 tcaaagcctg cggcgagtgc ttcaaattct gaatcgtggc acgcacggcg tatagccgca
     4021 tgagcggatg gcgaatgccg atgggcagat cgacgaacac caatccgaag cgatttccca
     4081 tgcttggctg cggatcgccg gcgccgcgca ggttcaccgg cacggcggcc cgtatcgtca
     4141 cccccgggac cgggtatccg cgtgattcaa gataacggcc gagggcgccg gcgagcgtgg
     4201 acatcaatac atcgttgacg gtgcacgata gaacgtggct tatcgtgcga acctcctgca
     4261 gcggcagcgc atcggtccat gctgcgcggc ggatccccga catcggccgt ttgagcggcg
     4321 ttgcggggtc atcggcgagc agtcccagat gcgcgagctc agcggccatt cccagtgctt
     4381 ggcgggcgag ggtcgaggtc tccctcggat ggagcgtata gtgaacggcc attccggtca
     4441 ggttggctcc gccttgcagg atctccaaga cgcggtcggg taaccagccg ctcgatgaat
     4501 ctgtccggct cgccgccgca gaatgcacgg tcgatgaatt cgagtgattt cccttgcgct
     4561 cgccgtccgc aagcgacagc agcacttgca cgagcgcaac tccgtccgca taggcgtggt
     4621 ggatccgcac gatgacggcg ctgcggccgt cgaagttctc cacgagatgg aacatccacg
     4681 gcggtcggcc cggattgaac ggcgtgctcg cgagctcccc gacgagcgcc tgcagttcct
     4741 gctggcccgc gggcaggggc aaggcgcagc tcagcacatg atcgtcaaga tcgaactgcg
     4801 ccgactcctg ccacgcagcg gcgatgcggc cattggccgg gacagagcga aaccgcgaga
     4861 aggcgagaaa ccgccgttcg atcaatttgc gcagcgctgc gcgctcaatg cgggcgtcga
     4921 gcaccagcaa gccgacgatc atcatcggat gcgacggccg atccatcagc agccacgctc
     4981 gatccacact cgacatgttc tcgacggcgc tcatggctac agtgtgatcc attcaagcga
     5041 cttttgatcc ctcggtttgt cctgatacca atgcggtact tcaggtgcgt atcgttgatc
     5101 agtctgacgt ttgggatgca aggcaatggc ttacttcgtc actggcggta ctggtttcat
     5161 aggccggcac ttcatcgctg agatttcagt gcacggtgag ccaatttatg tgctggtgag
     5221 acccgggtcg cgcactcgat tcgagcgcct cgtgaaggac aacggtgtgc gtggcagctt
     5281 gatcatcgcc gtggagggtg atttgagcga tgagttgctc ggcgtgccag cgtcaatgcg
     5341 cgagcagctg cgtggccaca tcggtcactt cgttcacttg ggtgcgctct acgacttggc
     5401 cgccgaagcg gctccgctcg agcgcagcaa tgtggctgga actcgaaacg cgctgcaact
     5461 tgcgcacgac ctcaaggccg gctgctttca ccaagtaagc tcgatcgcgg ccgccggtcg
     5521 ctatgagggg attttcaccg agtcgatgtt tgaccaagcc caggacctcg agcatcccta
     5581 ctttcgtacc aagcacgaat ccgaggcgct ggtgcgaagc tcgtgtcgaa ttccgtggcg
     5641 catctaccgg cccggcatgg tcgtcggaca ctccgtgagc ggtgcgatgg acaaggtcga
     5701 cggcccttac tacctgttca agctgattca gaaggtacgc gacaccgttc cgtcctggct
     5761 gccgctcgtc gggttcgagg gagggcacat caatctggtg cccgtcgact tcgtcgccgc
     5821 ggccctcgcc cacttgatgc acgcgccggg acaggatggt caatgctttc atctcacgga
     5881 tccgcaggat cgacgcatcg gcgacgtact gaacctgttt gccaaggctg cgcacgctcc
     5941 caccatgaca ctgcggctcg actcggacct gctgcagcgg ctcgcctcgg ccgtcggcgc
     6001 agtcagcgag ctgggtcatc cgctgcgccg cctcaaggtt cgagtgctgc gggagttcgg
     6061 cattcccgcc ccggtggtct cgctgctcga ctacccgacc cgatttgacg cctcgcgagc
     6121 gcaggaattg ctggccgccg ctggcattca agtgccgtgc ctggagaatt acgcctggcg
     6181 cctgtgggac tactgggagc gtcagctcga ttcggatctc gacacggtgg gcaacctgcg
     6241 ggccgcggcc gggggcaaaa ccatcctgat tacgggcggt tcatcgggta tcggccgcgc
     6301 tagcgccgtg aagctcgccg acgccggagc tcatgtcctc atcgtcgggc gcaatgtcga
     6361 gaatctgact cgcgtgagca gtgagatcga acggcgcggc ggccgcgtca gccaatacgc
     6421 ctgcgacttg acggatgcgg cggcgagcgc aaatttgctg gccaccttgc tggccgaaca
     6481 tggccacatc gacattctca taaacaacgc cggccgctcg attcgacgct ccatcgacaa
     6541 cagctacgat cgaatgcacg actacgaacg attgatgagc atcaactact ttgccgcagt
     6601 gcgcatgacc cttggcctgc tgccggcaat ggtcaagcat ggatcgggcc acgtcatctg
     6661 catctcatcc attggcgtga tcacgaatgc ggcacgcttc gccgcctaca atgcgtccaa
     6721 ggcggctctc gaagcattca cgcgctgtgc cgcggccgag tacagcgacc gcaatatttg
     6781 tttcaccgtc atcaacatgc ccctggtgcg cactccgatg attgcgccca ccaagttgta
     6841 tgagcgattc cacgtactgc agccagagca ggccgccgac caggtgtgtg ccgcggtcgt
     6901 gcatcggccg gcgcgcctcg cgactaggct ggggacgctt gcgcaggtcg tcgaagcgtt
     6961 cgctccgcgg atcaacacgg ccgtcatgag cgaggggttc acgatgtttc ccgaatccga
     7021 agccgccggc ggctcatctc cagccgctcg gccggcagag gcctcagccg ttgcattcgc
     7081 cgcgctcatg cgcggcgccc attggtgaat ggacttaggc ggcgcgcaca gcttcttcac
     7141 gcgcgagttc gcgccgcaag atcttgccga cgttcgtttt gggcagtatg gaacgaaact
     7201 cgacatgttt gggaatcttg tagccggtca ggcgttggcg ggaatgctcg cgcagatcct
     7261 ccggagtgag cgagcagcct tcgcgcagca cgacgaaaat cttcactgct tcgcccgaat
     7321 gcgcgtcggg cacgccgatg catccgcatt ccaataccgc ggggtgctcg ccgaccgcag
     7381 cctcgatttc gttgggaaag accttgaacc cggagacgat gatgagatct tttttccgat
     7441 cgacgatgcg cagtaatccg tcggctgcca tgaccgcaat gtcgccggtg cgcagccagc
     7501 cgtccggcgt cagcgtcttc gcagtgtcct cggcgcggtt ccagtagccg cgcatcacct
     7561 gcggcccgcg cacgcaaagc tcgcccgggt ctccgagcgg tacctcgacc tcatcgttgc
     7621 gaatgctgat ttccgtcgaa ggcagcggca ggccaacggt tcccaactgc ggcgcctcat
     7681 agctgttgca cgagacgacc ggcgaggact ccgtgagccc atagccctcg gccagcggcc
     7741 gtcccgtgag cgcgcgccat ctctcggcga cgctgggatg cagcgccatg cctccggccg
     7801 cgccgagctt cagatgtgag aagtcaagct tcgcgaaact cgggtggccc agcaaggcgc
     7861 tgtagagcgt gttgacgccg gtgatgatcg aaaatttcca gctgcggagt tcgtgcacga
     7921 acgcgccgat gtctcgaggg ttggtgatga gcacgttgag gccgccatac cgcatgaaga
     7981 ccaggcaatt gcacgtcagg cagaagatgt gatagagcgg caggggcgta atgatcacct
     8041 cacggccggg ctcgatcacg ttcttccaga actcgcccac ctgcagcaga ttcgcgacca
     8101 tgttgcgatg agtgagcacc gctcccttgg cgacgccggt cgtgcctccc gtgtattgca
     8161 ggaacgcaac atccgcagga ccgattactg gcgggtgcca tttgccgcgc gcgccgcgct
     8221 tcagcgcgga gcggaagggg cgcgcatcgg gcaaatcgta tgccggcacc agccgtttga
     8281 ctcgccgcac caggaaattc acgacgctgc gcttcgggaa tggcgccaaa tcgccgagac
     8341 tcgcaatcac gatgtgttgg atttgcgtag cggcgcgcac ctgcgccagg agatgcgcga
     8401 aattctcgag aatcacgatg gcgcgggcgc cggagtcgcg tagctgatgg cgcagttccg
     8461 gcggcgtgta aagcggattc acgtttacga cggtcagtcc cgcgcgcagc gccccgaaca
     8521 tggcgatcgg gtagtgcaaa acgttcggca tcattagcgc cacccgatcg ccgggctgca
     8581 gcccgagggc gagaagataa gcgccaaaat atcgcgattg ccgctcgagg tcgcgataac
     8641 tcagcgtcac gccgagatta tgaaacgcgg gacgctcggc gaagcgccgg caactctcct
     8701 cgagcaggtg aacgagcgaa gggtgagaat ccgcatcgat ctccgcggca attccgtgcg
     8761 ggtacgcctt gagccagatt ttatccattg agccactccc ccaacagcca actgattgag
     8821 cctcatctag ccccctgaat ttttattttg gcaatctgca aaagtgctta atgcctaagg
     8881 aattgctgat atcgctgcgg ccaatacgta tggtcgctag tgatcgccag cgtagactca
     8941 ccactgtttc atgaggtccg gtttattggc ggaaaacaag aaaagtcggg tttgtctttt
     9001 cgagacagcg ggggaaagca atcatgttca agtcattggc gatattcggc attacgctgg
     9061 tcgccgcgct gctttgcgcg tgcggatcga gcagtcagac ctcgagtgtc gccgcctcga
     9121 ccgcgcacgg gaccttgatt gagaatccgc cgtttcgcat cgccagctcg acgctgccac
     9181 cttccaggcg gagctcgccg ggaccagcag cggcgcacag ctgctgcaaa tcagcggcag
     9241 cccgagctgc ggggtggatt tctactacgt caagctctgg accgtggggg ccgcgggcga
     9301 gagcaccgag tcgtccggcg cgctgatggt gcccacgggt gcgccgcccg cttgctccgg
     9361 cgcgcgtccc atcgtgcttt atgcgcacgg tacgaacagc gacaagacgc tgaacatcgc
     9421 cgatgtcacc aacgccacca ataccgaggg cgtgttgatt gcggctatgt tcgcggcgca
     9481 gggctacatc gtggtggcac caaattacgc cggctacgac atctcgcgcc tgggctatcc
     9541 cccctacctc aatgccgaac aacaatctgg agaaatgctc gacatgttga ccgcggcgcg
     9601 cgcggcactg ccgaagaccc tgagcgcggc gaccagcggc gaaggcgccg gcgcagccgc
     9661 tgcgcctggc gttttacaag aatgatctgc gcaatggtgc gtgggctcca acgtccccca
     9721 ccttgctgtg cggtggcgac gcggatccaa cggtgttttt cccggtgaac acgggtgtca
     9781 tggcggaatt ctggagtccg ctggtgcaag ccgggctcat tacggtgctg gacgtgaacg
     9841 gcacaccctc ggggccgttc gcggccttgc agacgggctt ccaggaaagc caggcggcgc
     9901 agctcgccta ctacgcatcg gcggccgggg gccgcctgat gccggcggcg gcacagcaac
     9961 tcatcgtgga gggataccac acttccgtgg cgcccttctg ctcagcggcg gcgcgagcct
    10021 ttttcggcca actgtaaggg
//
LOCUS       DASWZT010000463         7370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_90646, whole genome shotgun sequence.
ACCESSION   DASWZT010000463 DASWZT010000000
VERSION     DASWZT010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7370
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90646"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VGE92_13265"
     CDS             <1..896
                     /locus_tag="VGE92_13265"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY1314844.1"
                     /translation="EGGAVKKAVVGIYPGPKGHWVGDGFPVRSLFTYDGLGQRLSPFL
                     LLDYAGPAEFPAAARPRGVGEHPHRGFETVTIVYQGEVAHRDSVGNAGLIGPGDVQWM
                     TAASGILHEEFHSPAFTRSGGALEMVQLWVNLPAEHKMSAPRYQTIRDAQIPRVELPA
                     GAGSVRVIAGSLDAEAGPAATFSALNVWDVRLRSQRKASLPVPTGHIAAIAVLRGSIA
                     VNGSSRAGDAELVVLDAAGGEIEIEADTDSTLLLLSGVPIGEPIVGYGPFVMNTRQEI
                     ETAMRDFQSGKFGRMPVSATR"
     gene            943..1953
                     /locus_tag="VGE92_13270"
     CDS             943..1953
                     /locus_tag="VGE92_13270"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY1314845.1"
                     /translation="MQSTLSQRKLGPFAVSSIGLGCMNLSHAYGVPPSPQAAARILIR
                     ALDSGVTHFDTAALYGFGANEELLGRTLSPFRSRFTLASKCGIQGVSSEAGVRRVIDG
                     RAQTLKQTCEDSLRRLKTEVIDLYYLHRWQKSVPIEESVGALGELVRAGKIRAIGLSE
                     VSAATLERAHAVHPIAAVQSEYSLCTRNPEIFLIEACRRIGATFVAFSPLARGFLAGA
                     VRDVATLAAKDLRRTMPRFEAAANAANLRLLEDFAGLALGAQASMAQLALAWVLTRGA
                     HVVAIPGTTSEAHLLENLQAAGLQLEPEVVARADALINRHTIAGDRYNAATQAEIDSE
                     NF"
     gene            1972..3363
                     /locus_tag="VGE92_13275"
     CDS             1972..3363
                     /locus_tag="VGE92_13275"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HEY1314846.1"
                     /translation="MISRRDLLMVSGLVGLTSSFTEMARGLNVRAADAPDYTIDIAPL
                     SIELSPRHKLTTTAYNGQVPGPLLRFKENQPIKIDVFNRTDRPEVVHWHGLDTPPEAD
                     GAVEEGTLPIAPNSSARYTFTPRPSGFRWYHTHTMAMNDFTRAQYSGQHGFLMIEPRQ
                     NSAPYDQEFFLALHDWQGYLLASDDGAMNPSYAVSTINGKAMGSGEPLRVKQGQRILL
                     HVLNSSPTEVHWIALSGHSFRVVALDGNPVSNPKTVPMLRLAPAERVCALVEMDNPGV
                     WVLGEVRKHVQALGMAIAVEYAGKKANPQWQQPAELIWNYEQFAGSDLGAAAVEPATL
                     IPLVFESTFQGHGAMEKWTINGASYPTARVAPLVQGRRYRLQFINKSVDDHPLHLHRH
                     SFELRRLRAPLSSAAGAAPNDIRGIVKDVVLVDAQTRAEVEFTANNPGASLFHCHQQN
                     HMDLGFMMLFTYA"
     gene            3356..4168
                     /locus_tag="VGE92_13280"
     CDS             3356..4168
                     /locus_tag="VGE92_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314847.1"
                     /translation="MPERSFSTRFLFYAIDLLAVAPCVAQAQTNYEIQVYGAETVAPE
                     TLMVELHSNFTVDGQKYLMDGVYPTNHQEHETLELTQGINDWSEVGFYIFTSEQDGHG
                     VQWVGDHIRPRIRVPDSWGWPVGVSLSTEIGFQRSAYSTDTWTWEIRPIIDKSIGRWY
                     FAINPALERTLHGPDVNQGLGFAPSAKVSYDFTRVVSAGVEWYADYGAIGNIAPLHDQ
                     QQQVFVVTDLNVSPLWEINIGIGIGATGSTDHLIVKAILGRRFDWGRHSPTD"
     gene            4274..5290
                     /locus_tag="VGE92_13285"
     CDS             4274..5290
                     /locus_tag="VGE92_13285"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEY1314848.1"
                     /translation="MKKTAGGIFESRHRREAKAGAGLALIGLLALCPAWADDTAAPAA
                     TSGSEAIEELAELMVEAPEPRYVSPTRRDQIGRIWAPVMINGRGPFRLVLDTGASHSA
                     VTSLVALALGIPMDQSPPVILRGVTGSATVPTIHVDTLTVGDLSVDSPMLPIVPDALG
                     GAQGILGSEGLAGKRIFIDFRHDKIQITFSRNERSTRDFVDIPFRAIRNTLVVVNATI
                     GDVHTKAIIDTGGQATIANLALRDALARNGGPLHGKADQIIGATKDIQDGVIMAMPAI
                     EFGSIQIRDSGVTFADMYIFKQWKLEKEPAILIGMDALGLLDTLVIDYRRHELQMRMP
                     RGAG"
     gene            5306..6100
                     /locus_tag="VGE92_13290"
     CDS             5306..6100
                     /locus_tag="VGE92_13290"
                     /inference="COORDINATES: protein motif:HMM:NF022665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HEY1314849.1"
                     /translation="MINKPIIGVAAAVVIAAAGTWYYLHARHAALPAAPAAVQLPVPV
                     PPAEDAIQHPLAAAEDSAVKAPLPALNDSDAALSEALRGVIGPSALQDFLLPENIIRH
                     IVVTIDNLPRPKAAVAKRPTSAVGGTFVVDGDELRAKLDPQNFARYQPMVAVISKLDM
                     HQLVGVYGHFYPLFQQSYQDLGYPNGYFNDRLVQVIDNLLATPAPAEPIELLRPNVMY
                     TFADPKLESLSAGQKILIRMGPDNAAAIKIKLKELRAAVTATPPKR"
     gene            6333..7340
                     /locus_tag="VGE92_13295"
     CDS             6333..7340
                     /locus_tag="VGE92_13295"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HEY1314850.1"
                     /translation="MKTSVKNSKHTLMSAGLVLSLSAVAISGCVVATAPPPPPRRVYV
                     APPPPPPPPPPVVYAEPAVDVEVRASEAPPPLPDYDQPPCPEDGYLWTPGYWAWGGGG
                     YYWVPGTWVQPPRVGVLWTPGYWGFIGGVYAFHAGYWGPHIGFYGGVNYGFGYVGVGF
                     AGGHWAGNSFAYNQSVTNVNVTNIHNTYNETVINNVTVNKVSYNGGAGGVAAVPTAQE
                     KAAATEPHAAPTPMQRQHVQQAAANPALAAKANGGHPAIAATPRPAAFNAPGVVGARG
                     AAMSPRVAAAQTNPGQTNAAAHPQAVPPKAPPPAGKAPPKQQQHPKPPKTEKHGNETP
                     R"
BASE COUNT         1414 a         2269 c         2293 g         1394 t
ORIGIN      
        1 gcgagggcgg tgcggtgaaa aaagcggtag tgggcattta tccgggcccc aaggggcact
       61 gggtgggcga cggatttccg gtgcgcagcc tgtttacata tgatggtcta ggccagcgcc
      121 taagcccctt cttgctgctg gattatgcgg gcccagccga gttcccagcg gcggcgcgcc
      181 cgcgcggcgt gggcgagcac ccgcatcggg gcttcgaaac agtaactatc gtctatcagg
      241 gagaggtcgc gcaccgcgac tctgtgggaa atgccggatt gatcggtcct ggagacgtgc
      301 agtggatgac cgcggcatcg ggaatcttgc acgaagagtt tcattctccc gctttcacgc
      361 gcagcggcgg cgccttggag atggtgcagc tctgggtcaa tctgccggcc gagcacaaga
      421 tgtcggcacc ccggtatcaa accatccgcg acgcgcaaat tccgcgcgtc gagcttcccg
      481 ccggtgccgg atcggttcgt gtaatcgccg gcagcctcga cgctgaggcc ggtcccgccg
      541 ccacgttcag tgcgctcaat gtttgggacg tacgcttgcg ctcgcagcgc aaggcgtcgc
      601 tgccggtgcc gacgggccac atcgcagcca ttgccgtgtt gcgcggctcg attgccgtca
      661 acggttcaag ccgggcgggc gatgccgaac tggtggtgct ggacgccgcc ggcggcgaga
      721 ttgagatcga agccgacacg gacagtacgc tgttgctgct ctccggggtg ccgatcggcg
      781 aacccatcgt tggctacggc ccattcgtga tgaacacgcg ccaggaaatt gaaaccgcga
      841 tgcgcgattt tcaaagcggc aagttcggcc gtatgccggt gagcgcaaca cgctagaacc
      901 tccagcaggc gaatcacgcg cgtgggtgag aacgggaacg caatgcaatc gaccttgagc
      961 caacgcaagc tcggcccctt cgccgtctcc tcgatcggtc tcggctgcat gaacttgagt
     1021 catgcctacg gtgtgccgcc gtcgccgcaa gctgcggcgc gcatcctgat tcgcgccctg
     1081 gactcgggtg tgacgcactt cgacacggcg gcgttgtacg gttttggcgc gaacgaggaa
     1141 ttgctcgggc gcacgctctc gccgtttcgg tcgcgattca ccctggcgag caagtgcggc
     1201 atccaaggcg tgagctcgga ggctggcgtg cgccgcgtca tcgacggccg tgcacaaacc
     1261 ctcaaacaaa cttgcgagga tagtttgcgg cgcctgaaga ccgaggtcat cgatctttac
     1321 tatttgcatc gttggcagaa gagcgttccc atcgaggaga gcgtgggcgc gctcggggaa
     1381 ttggtgcgcg cggggaagat tcgcgccatc ggcctgtccg aggtgtcggc ggcgacgctc
     1441 gagcgtgcgc atgcggtaca tccgatcgcg gcggtacaaa gcgaatattc gctgtgcaca
     1501 cgcaaccccg aaattttttt gatcgaggcc tgccggcgca tcggcgcaac attcgtcgcc
     1561 ttcagtccgt tggcgcgggg atttctcgcc ggcgcggtgc gcgacgtcgc caccttggcg
     1621 gccaaggacc tgcggcgcac gatgccgcga ttcgaggcgg cggcaaacgc tgcgaatctg
     1681 cgtttgttgg aagatttcgc gggactcgcg ctcggcgcgc aggcctccat ggcgcaattg
     1741 gcgctcgctt gggtgctgac tcgcggcgcg cacgtggtgg cgatcccagg cacaacctcc
     1801 gaggcacatt tgctggagaa tcttcaggcc gcggggttgc agctcgagcc cgaggtcgtc
     1861 gcgcgagcgg acgcgctcat caatcgacac acgatcgccg gcgaccgcta taacgccgcc
     1921 actcaggccg agatcgattc cgagaatttt tgacttcgtt accattccac catgatttcg
     1981 cgacgcgatc ttttgatggt ttcgggcctc gtcggactga cctcatcatt caccgagatg
     2041 gcgcgcggtt tgaatgttcg cgcggcggat gcgccggatt acaccatcga cattgcgccc
     2101 ctgagcatcg agctatcccc gcgccataaa ttgacaacca ccgcgtacaa cggccaggtc
     2161 ccgggtccgc tgttgcgctt caaggagaat caaccgatca agatcgacgt gttcaatcgc
     2221 accgatcgtc ccgaggtggt gcactggcac ggtctcgaca ccccgcccga ggccgatggc
     2281 gccgtggagg aaggcacgct gccaatcgcg ccgaattcga gcgcgcgtta tacatttact
     2341 ccacgtccgt cagggtttcg ctggtatcac acccacacca tggcgatgaa tgatttcacg
     2401 cgtgcgcaat acagcggcca acacggcttc ttgatgatcg agccgcgcca aaattcagcg
     2461 ccttacgatc aggagttctt tcttgctctg cacgactggc agggctattt gctcgcgagc
     2521 gacgacgggg ccatgaaccc gagctatgcg gtgagcacca tcaacggcaa agccatgggc
     2581 tcgggcgaac cgctgcgggt caaacagggg cagcggatct tgctgcatgt tctcaactcg
     2641 agtccaactg aagtgcattg gatcgccttg tcggggcaca gttttcgcgt cgtggctctc
     2701 gatggcaatc cggtttccaa tccgaaaacg gtaccgatgt tgcgcctggc acccgccgag
     2761 cgggtgtgcg ccctggtgga gatggacaat cctggcgttt gggtgctggg cgaggtgcgt
     2821 aagcacgtcc aagcactcgg tatggcgatc gctgtcgagt acgcaggcaa aaaagccaat
     2881 ccgcagtggc agcagccagc tgagctcatc tggaactacg aacaattcgc gggctcggac
     2941 ctcggcgcag ccgcggtgga acctgcgacc ctcattccgc tggtgttcga atctacgttc
     3001 caaggtcatg gcgccatgga gaaatggacc atcaatggcg cctcctatcc gaccgcccga
     3061 gtggcgccgc ttgtccaggg gcggcgctac cgcttgcaat tcatcaataa gagcgtcgat
     3121 gaccatccgc tgcatctaca ccggcatagt ttcgagttgc ggcgcttgcg ggcgccattg
     3181 agcagcgctg caggggcggc accgaacgat attcgaggca tcgtcaaaga tgtcgtgctg
     3241 gtcgacgcgc agacgcgtgc cgaagtcgag ttcacggcga acaatccggg tgcttcgctg
     3301 tttcactgtc atcagcaaaa ccacatggat ttgggcttta tgatgctctt cacctatgcc
     3361 tgagcgatct ttctccacgc gctttttgtt ttatgcaatt gacctgctgg cggtcgcgcc
     3421 atgcgttgcc caagcacaaa cgaactacga gattcaggtc tacggcgccg agactgtcgc
     3481 ccctgagacc ttgatggtcg aactgcattc gaatttcacg gttgatggcc aaaagtacct
     3541 catggacggt gtttatccga cgaatcatca ggagcacgag acgctcgaac tcacgcaggg
     3601 catcaatgac tggtccgagg tcggattcta tattttcacg agcgagcagg atggacacgg
     3661 cgtgcagtgg gtcggcgatc acattcgtcc gcgcatacgg gtcccggact catggggctg
     3721 gcccgtgggt gtgagtcttt caactgaaat tggctttcaa cgctctgctt actcgaccga
     3781 cacctggacg tgggagatcc gtcccatcat agacaagagc attggacgct ggtatttcgc
     3841 catcaatccg gccttggagc gcactttgca tggcccggac gtcaatcaag gtctcggttt
     3901 cgcaccgagc gccaaggtta gttatgattt cactcgcgtc gtcagcgccg gcgtcgagtg
     3961 gtatgccgac tacggtgcca tcggcaacat cgccccgtta cacgatcagc aacagcaagt
     4021 cttcgttgtc accgacctca atgtgtcacc cctctgggag atcaacatcg gcattggcat
     4081 cggtgccacc ggttctaccg atcatttgat cgtaaaagcc atcctcggtc ggcgattcga
     4141 ctggggccgg cattcgccaa ccgattgaag ccacagcttg cgcgcaggat tcgcaatacg
     4201 tgaatcgatc cgcaatctta cgttggtcgc cgccaccccc ggtgctatgc ttggcgccaa
     4261 ggcggttgca gcattgaaga agacggctgg cggtattttc gaatcacggc atcgccgcga
     4321 ggcgaaggct ggcgccggct tggcgctgat cggcctgctg gcgctgtgcc ccgcatgggc
     4381 cgacgatacc gctgcgccgg cggcaacttc cggctccgag gccatcgaag agctcgcgga
     4441 actcatggtc gaggcacccg agccgcgtta cgtttcgccg acgcgacgcg atcaaatcgg
     4501 tcggatctgg gcgccggtca tgatcaacgg ccgcgggccg tttcgcctgg tgctcgacac
     4561 cggcgcgagt cacagcgcgg tcacctcgct ggtggcgctg gcgctcggaa ttccgatgga
     4621 ccaatcgccg ccggtcatct tgcgcggagt caccggctcg gccacggtgc cgaccatcca
     4681 cgtcgatacg ttgaccgtcg gcgatctgtc ggtggactcg ccgatgttgc ccatcgtgcc
     4741 ggatgcgctc ggcggcgcgc agggcatcct cggatccgaa ggactggcag gaaaacggat
     4801 tttcatcgat tttcgccacg acaagatcca gatcaccttt tcgcgaaacg agagatcgac
     4861 gcgcgatttc gtcgacatcc cgtttcgcgc gatacgcaat acattggtgg tggtgaacgc
     4921 gacgatcggc gatgtacaca ccaaggccat catcgatacc ggtggccagg cgaccatcgc
     4981 gaatttagcg ttgcgggatg cgctggcccg caatggcgga cccctgcacg gcaaagcgga
     5041 tcaaatcatc ggcgcgacca aggacattca ggatggcgtg atcatggcca tgccggcgat
     5101 agaattcggc tcgattcaga ttcgcgacag cggggttacc ttcgccgaca tgtatatttt
     5161 caagcagtgg aagctcgaga aggaaccggc gatattgatc ggcatggatg ctttgggatt
     5221 gctcgatacg ctggtcatcg attatcgccg ccatgaactg caaatgcgca tgccaagggg
     5281 agcgggctaa ggtccgaaag cacgcatgat caacaagccg ataatcggag tggccgccgc
     5341 ggtcgtcatc gccgcggcgg gtacctggta ctacctgcac gcacgccacg cggcgctgcc
     5401 tgccgcaccg gcggcagtgc agctgccggt gcccgtgccg ccggctgaag atgcgattca
     5461 acatccattg gcggcggcgg aggactcggc cgtcaaggcg ccgctgccgg cgctgaacga
     5521 cagcgatgcg gccttgagcg aggcattgcg aggggtcatt gggccgagcg cgctgcagga
     5581 tttcttgctg ccggaaaaca tcatccgcca catcgttgtc acaatcgaca atttgccgcg
     5641 gccgaaggcg gcggttgcga aacggccgac cagtgcggtc ggcggcacgt tcgtggtcga
     5701 cggcgatgag ctgcgagcca agctcgatcc acagaacttc gcgcgctatc agccgatggt
     5761 ggccgtgatc agcaaactcg acatgcatca gctggtgggc gtttacgggc atttctaccc
     5821 gctttttcag caatcctatc aagacctcgg ctatccgaac ggctacttca acgatcgtct
     5881 ggtgcaagtg atcgacaact tgttggcgac gcctgcgccc gccgaaccga tcgagttgct
     5941 gcggccgaac gtcatgtata cttttgccga tccgaaactc gagtcgctgt cggccgggca
     6001 gaagatcctg atcaggatgg gtccggacaa tgcggccgca ataaaaatta aactcaaaga
     6061 gctgcgagct gcggttaccg ccaccccgcc aaagcgataa actgcctcgg ccggcaggac
     6121 taattcaaga cgcgagatcc catggctggc gtaaacccgc cccgctcgct gcgtgcgatg
     6181 ccgaggcgcg ggtgtcgctt tacggggaca ggacgcgtta caaacgcttc attgaagtag
     6241 tcgtttctca gagtgtgacg ttatatgttc tatcgacggc taactgttaa gtacgctggc
     6301 gcacaacacc acacacactc ggcgggtact caatgaaaac atcggtcaag aattcgaaac
     6361 acaccttgat gagcgcgggc ctcgtgctgt cattgtcggc agtcgcgatc agcggttgcg
     6421 tggtcgcgac cgcgccgccg ccgccgccgc ggcgcgtgta tgtggcgccg ccgccgccgc
     6481 cgcccccgcc tccgcccgtg gtgtacgccg agccggcggt cgacgtcgag gttcgagcca
     6541 gcgaagcgcc tccgccctta ccggattacg atcaaccgcc ctgcccggaa gatggatatc
     6601 tgtggacccc cggctactgg gcctggggcg gcgggggata ttactgggtg ccgggcacct
     6661 gggtgcagcc gccgcgagtc ggcgtgctct ggacccccgg ctactggggc ttcatcggcg
     6721 gtgtttacgc attccacgcc ggttactggg gtcctcacat cggcttttac ggcggcgtga
     6781 actacggatt tggctacgtg ggcgtcggct ttgccggcgg acactgggcg ggcaattcct
     6841 tcgcctacaa ccagagcgtc accaacgtca acgtcaccaa tatccacaat acctacaacg
     6901 aaaccgtcat caacaatgtc accgtcaaca aggtgagcta taacggcggt gccggcggcg
     6961 ttgccgcggt accgaccgcg caagaaaaag ccgccgcgac cgagccgcac gcggcgccca
     7021 ccccgatgca gcgccagcac gtgcagcaag cggccgcaaa tccggcattg gccgccaagg
     7081 ccaacggcgg acatcccgcg atcgccgcca cgccgcgacc cgcggcgttc aacgcgccgg
     7141 gagtcgtggg tgcgcgtggc gctgcaatga gcccgcgcgt cgccgcggca cagaccaatc
     7201 ccggccagac gaatgcggcc gcgcatccgc aggcggtccc gcccaaggct ccgccgccgg
     7261 ctggcaaagc gccgccgaag cagcaacagc atccgaagcc gccgaagacg gaaaagcacg
     7321 ggaacgaaac gcctcgatag accccgcgcg cgagtcggac attgtccgac
//
LOCUS       DASWZT010000464         5077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_85891, whole genome shotgun sequence.
ACCESSION   DASWZT010000464 DASWZT010000000
VERSION     DASWZT010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85891"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1566
                     /locus_tag="VGE92_13300"
     CDS             <1..1566
                     /locus_tag="VGE92_13300"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1314851.1"
                     /translation="VLAFGALIVVSALLFPLLGQDFFPQVDAGQMRLHVRAPAGTRLE
                     RTQQYFAEVERYIRKMVGDDQIDVMLDNIGLPYSGINIALSDSATVGPMDGEILISLA
                     KKHMPTAKIVAELRRELPARFAQLQFFFQPADIVDQVLNFGQPAPIDVRIAGPDQDAA
                     FALASQIARALQRVAGVVDSHVFQVPDAPSLTVDMDRALASETGVTQQEAANNVLVAT
                     NSSAQTAPNFWVDPRNSVSYPLVVQVPTYRISSSQDLKTMPVTSAEGATSGQLLSNVA
                     AFGREAVPMVTSQLNIRPVFDVHADVQGRDLSSAARDIDKVLQAHRPNASTALTVTLS
                     GQVATMRESYSGLFTGIALAIVLVYLFLVMNFQSWIDPLIVLMAVPFALSGVLWMLFL
                     SQTPLSVPALMGTLMCIGLTTANSILVVSFANQRVAAGDTPLRAATSAGYTRLRPVLM
                     TAGAMILGMVPMALGIGEGGEQNAPLGRAVIGGLLFATMATLIFVPTMYRLMRRQAAA
                     VNNLAAKGAANAI"
     gene            1556..2854
                     /locus_tag="VGE92_13305"
     CDS             1556..2854
                     /locus_tag="VGE92_13305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY1314852.1"
                     /translation="MPSDDPPHPEPARATNGDPSRSEFPDEVSRSEQPDASVSFASGT
                     GERTRRAVGIAAALLLVGFLAAIVLRLFHAHSVASAGEAEYAAPPPVDVVSARAPSLG
                     QDLVLPGETAAWYETTIYARVNGYVEKWLVDIGDHVKQGQILATIETPELDAELAAAR
                     AQLKASEAQLAARKAETEFSKTTNERWRDSPKGVVSEQEKESKKADYQSAEARQSAAV
                     AQVALDESKVDQYIALTEFKQVKAPFDGTITERKIDVGNLVTAGSGSTTTSMYRMAQT
                     DPLRIFVEVPQSAAGELMHAGVPAEIRASGAVGGVFDGKIARSAESVNAQARTMRVEV
                     DMPNGTHALVPGMYVNVAFRLPPRGLVEVPAAALIFRAGGTQVARVDASGKIEFADAT
                     IARDNGSMVELASGAKPGDRLVLNISSQIGAGQKVAVNTQ"
     gene            2851..4362
                     /locus_tag="VGE92_13310"
     CDS             2851..4362
                     /locus_tag="VGE92_13310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY1314853.1"
                     /translation="MKTLRVMALGLAASLGACAVGPSYRTPKSDAPPQFAVPVVSKAG
                     AAAPDLAAWWRALHDPELDSLVERAVKSNLDLAIALDRLQQARTYEAVVVGHALPEVD
                     ASGAAARGTGSDLTRGRAQQALVSADNTSDLQHVNTIAGFDAVWELDIFGKFRREFQA
                     ARADTQAARAARYDVLTAVIADVVRAYIDLRGSQVRVGILHQAGDLLRGSLRIVTIRY
                     ERGITNELDVALATRELATLEAEIAPAEAQVNAAQYTLAVLVGEYPESMLEELAKPDL
                     IPTMPAPTAPGVPLDLLKRRPDIQQAERELAAATARIGVAAANLYPQVALVGAIGSQA
                     QGWGSMPALSKHIWSFGPGAMWPLLDFGALDAQMDIADLAAHASLMNYRKTILDAVQQ
                     VDTSVAAYEAEQARLEKLDTAMAAGQRAVDLATARYDRGLTDFLNVVDAERQFYDLQE
                     QYAAAQVAQGEQFVRLYKNLGGGWQNYQAVPAIRRPQPAVIAAFRRALSSSSP"
     gene            complement(4365..4688)
                     /locus_tag="VGE92_13315"
     CDS             complement(4365..4688)
                     /locus_tag="VGE92_13315"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY1314854.1"
                     /translation="MGRRFILAGLAPVAILAAGLCGCAAYRKCGAGGCQGDAAITAQV
                     QMLFKQHAELEPPNLLDVQTMDHVVYLYGLVDTDYERQMAESVALQASGVRKVVNSIG
                     LSGNR"
     gene            4886..>5077
                     /locus_tag="VGE92_13320"
     CDS             4886..>5077
                     /locus_tag="VGE92_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314855.1"
                     /translation="MRNGYLHPTPRASGVRGNRTQAALKNGCLFVGLMVATGLAQAQS
                     ASAPPAPTDDSLTWHGITLY"
BASE COUNT          968 a         1541 c         1657 g          911 t
ORIGIN      
        1 gtgctggcgt tcggcgcgtt gatcgtggtg tcggcgctgt tgtttccgtt gctgggacag
       61 gatttttttc cgcaggtcga tgccgggcaa atgcgtctgc acgtgagggc gccggcggga
      121 acccggctgg agaggacgca gcagtacttt gccgaggtcg agaggtacat ccgcaaaatg
      181 gtgggtgacg atcaaatcga cgtgatgctc gacaatatcg gattgcccta cagcggcatc
      241 aacattgcgt tgagcgattc ggccaccgtg ggaccgatgg acggtgaaat cttgatttcg
      301 ctggccaaga aacatatgcc caccgccaag atagtcgccg agttgcgccg tgaattgccg
      361 gcgcgattcg cgcagctgca gttcttcttc cagccggcgg acatcgtcga ccaggtgctc
      421 aatttcggac agccggcgcc gatcgacgtg cgcattgccg gacccgatca ggacgcggca
      481 tttgcgctgg catcgcagat cgcccgtgcg ctgcaacgcg tcgcgggagt cgtggactct
      541 cacgtatttc aagttcccga tgcaccctcg ctgacggtgg acatggaccg cgcactggcg
      601 agcgagaccg gtgtcacgca acaggaggcg gcgaacaatg tgctggtcgc gaccaattcg
      661 agcgcgcaaa cggcgccgaa tttctgggtg gacccgcgca acagcgtgag ctatccgttg
      721 gtagtccagg tcccgaccta ccgcatttcc tcctcgcagg acctcaaaac catgccggtg
      781 acctccgctg agggcgcgac ctccggccag ctgttgtcga acgtggccgc gttcggccgc
      841 gaggcggtgc cgatggtgac ttcgcaattg aatattcgcc cggtgttcga tgtacacgcc
      901 gacgtgcagg ggcgcgatct gagctcggct gcgcgagaca tcgacaaagt tctgcaggcg
      961 catcggccga atgcgtcgac ggcgctgact gtgaccttga gcggacaggt tgcgaccatg
     1021 cgcgaaagct acagcggatt atttaccggt atcgcgctcg ccatcgttct ggtttatctg
     1081 ttcttggtga tgaatttcca gagttggatc gatccgctga tcgtgctgat ggcagttcca
     1141 tttgcgttga gcggcgtgct gtggatgctg tttctgtcgc aaacgccgct gagcgttccg
     1201 gcgctcatgg gcacgctcat gtgcatcggc ttgacgaccg caaacagcat tctggtggtg
     1261 agtttcgcca atcaacgcgt ggccgcgggc gacacgccgt tgcgtgcggc gacgagcgcg
     1321 ggctacacgc gcttgcggcc ggtgctgatg accgccggcg cgatgattct cgggatggtg
     1381 ccgatggcgt tggggatagg cgagggcggc gaacagaacg cacctctggg cagagccgtg
     1441 atcggcgggc tgctgttcgc gaccatggcc acgttgatct tcgtccccac gatgtaccgc
     1501 ctgatgcggc gccaagcggc ggccgtcaac aacttggccg caaagggtgc cgcgaatgcc
     1561 atctgatgac ccgccccacc cggaacctgc tcgcgcaacg aacggggatc cgtcgcgcag
     1621 cgagtttccc gacgaggtgt cgcgttcgga acagcccgac gcgtcggtga gtttcgcctc
     1681 gggaaccggc gagcgcacca ggcgggccgt aggcatcgcg gccgcgctgt tgctggtggg
     1741 tttcctggcc gcgatcgtgc tgcgtctttt tcacgcacac tcggtggcaa gcgcgggcga
     1801 ggcagagtac gcggcacccc cgccggtcga tgtggtcagc gcacgggcgc cgagcctcgg
     1861 gcaggacttg gtcctgccgg gcgagactgc agcttggtac gagaccacga tctacgcgcg
     1921 ggtcaatggt tacgtggaaa agtggctggt ggatatcggc gatcacgtca aacagggcca
     1981 gattctcgcg acgatcgaaa ctccggagct cgatgccgag ctggccgcgg ctcgagcgca
     2041 gctcaaagcc tccgaggcgc agctggcggc tcgaaaagcg gagacggaat tcagcaaaac
     2101 gaccaatgaa cgctggcgcg attcgcccaa gggagtcgta tcggaacaag aaaaggaatc
     2161 caagaaagcc gactaccaga gtgcagaggc gcgtcagagt gccgccgtgg cgcaggtggc
     2221 gctcgacgag tccaaagtcg atcaatacat cgcgttgacc gaattcaaac aggtgaaggc
     2281 ccccttcgac ggaaccatca ccgaacgcaa gattgacgtc ggcaacctgg tgaccgcggg
     2341 cagcggctcg acgaccacgt ccatgtaccg catggcgcag accgatccgc tgcgaatttt
     2401 cgttgaggtt ccgcagagcg ccgccggcga actgatgcac gcgggcgtgc cggccgagat
     2461 ccgcgcgagc ggcgccgtcg gcggtgtctt cgacgggaaa atagccaggt ccgccgaatc
     2521 cgtcaacgcg caggcacgca cgatgcgcgt cgaagtggat atgccgaacg gcacgcatgc
     2581 gctggtacca ggaatgtacg tcaacgtggc attcaggctg ccgcctcgag gcttggtgga
     2641 agttccggcc gccgcgctga tttttcgcgc cggcggcacg caagtggcgc gggtggatgc
     2701 gagcggcaaa attgaatttg cggatgccac gatcgcgcgc gacaacggca gcatggtgga
     2761 attggcgtcc ggtgcgaagc ccggcgaccg cctggtattg aatatcagca gccagatcgg
     2821 tgcgggtcaa aaagtcgcgg tcaataccca gtgaagaccc tgcgcgtcat ggcactcggg
     2881 ttggcggcat cattgggcgc ctgcgcggtc gggcccagtt atcgcacgcc taaatccgat
     2941 gccccgccgc agttcgctgt ccccgtcgtg agcaaggccg gcgcggccgc gccggacctt
     3001 gcggcctggt ggcgggcgtt gcatgacccg gaactcgact cgctcgtcga acgtgcggtc
     3061 aagtcgaacc tggacctggc gattgcactc gatcgattgc aacaagcgcg cacctacgag
     3121 gcggtcgtcg tcggccacgc cttgccggag gtggacgcca gcggcgcggc cgcgcgcggc
     3181 acgggaagcg accttacccg cggccgcgca caacaggcgc tcgtttcggc cgataacacc
     3241 agcgatctgc agcacgtgaa tacgattgca ggttttgatg cggtgtggga actcgatata
     3301 tttggaaaat tccgcagaga gttccaggct gcacgcgccg acacgcaggc agctcgggcg
     3361 gcgcgctacg acgtgctgac cgcggtgatt gcggacgtgg tgcgcgccta tatcgacctg
     3421 cgcggctccc aggttcgggt gggcatcctg caccaggcgg gcgacttgct gcgcgggtcg
     3481 ctgcgcatcg tcaccattcg ctatgagcgt ggaatcacca acgagctcga tgtcgcgctt
     3541 gcaacccgcg agcttgcgac tttggaggcc gagatcgctc cagccgaggc acaagtcaat
     3601 gccgcgcaat acacgctggc agtgctggtc ggcgaatacc ccgaaagtat gctcgaagaa
     3661 ctggcgaaac ccgacttgat tccgacaatg cccgcgccga cagcccccgg ggtcccgctc
     3721 gacctgttga aacgccggcc ggacatccag caggcggagc gtgaactggc cgcggccacc
     3781 gcaaggatcg gcgtcgccgc ggccaatctg tatccgcagg tcgcgctcgt cggggcgatc
     3841 ggcagccaag cgcaggggtg ggggtccatg cctgcgctca gcaaacatat ctggtcgttc
     3901 ggtcccggcg cgatgtggcc gctgctcgac ttcggcgcac tcgacgccca gatggacatt
     3961 gccgatctgg ccgctcacgc cagcctgatg aactatcgca agaccatttt ggatgctgtc
     4021 cagcaggtcg acacgtcggt tgcggcctat gaagcggaac aggcgcgtct ggagaaattg
     4081 gacaccgcca tggcggcggg ccagcgcgcg gtcgatttgg cgactgcgcg gtatgaccgt
     4141 ggcctcaccg atttcctgaa cgtcgtggat gccgagcgcc agttctacga cctgcaagag
     4201 cagtacgcag ccgcacaagt ggcgcagggc gagcaattcg tacgactcta taaaaatcta
     4261 ggcggcgggt ggcagaacta tcaggcggtt cccgccatcc gacgtccgca gccggccgtt
     4321 atcgctgcgt ttcgtcgggc gctcagcagt tcaagtccct aggcctaccg attgccgctg
     4381 aggccgatcg agttcacgac ttttcgcacg ccgctcgctt gcagcgccac tgattccgcc
     4441 atctggcgtt cataatccgt gtcgaccaag ccgtacaaat agaccacgtg atccatggtc
     4501 tgcacgtcga gtagattggg cggctccaat tccgcgtgct gtttgaacaa catctgtacc
     4561 tgggcggtaa ttgccgcatc accctggcac ccgccggctc cgcatttgcg gtacgcggcg
     4621 cagccgcaga gacccgcggc gaggatcgcg acaggcgcca agccggcaag aatgaacctg
     4681 cgccccatga ggcagcgaag tgttccagtc gagagggtca tggcatgcat tggacccgac
     4741 tacggcgcca aaggtttcga atctcgccgc caccgcccaa cgaagcaatt gttgcaaaaa
     4801 aaagacatag gacctgggac tgcgtaatat tcttgtaaca ttgcgtgggt taaatccaac
     4861 gattgaaacc acgggagtaa tcattgtgag aaacggatac ctgcacccga cgccgagggc
     4921 atcgggagtt cgcggcaacc gcacccaggc tgcgctcaag aacggatgcc tattcgttgg
     4981 tttgatggtc gcaacaggtt tggctcaggc gcaaagcgcc tcggcgccgc cggccccgac
     5041 cgacgacagc ctgacgtggc acggcatcac cttgtac
//
LOCUS       DASWZT010000465         4638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_81214, whole genome shotgun sequence.
ACCESSION   DASWZT010000465 DASWZT010000000
VERSION     DASWZT010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81214"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VGE92_13325"
     CDS             complement(<1..723)
                     /locus_tag="VGE92_13325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY1314856.1"
                     /translation="MRNSPLVSIVLPTFNRLQYLPEAVASVLAQSYENWELLIADDGS
                     DTETCAYLQTLVDPPRVQLLRLPHSGKPAVARNRALRQANGEYVAFMDSDDVWMPDKL
                     SMQIASLRARGDCGWSHTQFVLVNAAKQSRREMPAADGWILGPLLRTETVIALPSVVV
                     ARTLLDEVGGFDEELVMCEDYDLWLRLAARSAIDAVPQPLTVVQRHARHYGDPATSFR
                     DSVRVLDKALRSGTAVPYEFFIR"
     gene            complement(720..1865)
                     /locus_tag="VGE92_13330"
     CDS             complement(720..1865)
                     /locus_tag="VGE92_13330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY1314857.1"
                     /translation="MRVAQISFFCDPQARAPEQLLQDWPSLGDVADCASRAGVRVSVI
                     QACGQSQDLRRAGVDYHFLPFGRAVYGGRARLAGRFHELLHSISPDVLHVHGLDFAAD
                     VLSLTAAAPGIPLLLQDHASRPPRAWRPWRRALWRRGFSAAAGIAFCSVEQARPFAAA
                     GLLRARAKLYAIPESSSRFTPGDKVKARGSSRVSGDPLILWVGHLDANKDPLVVLDGI
                     AKTARRLPHLQLYCCYGDTALLPAVRGRISGDPLLCGRVHLLGRVSHGEIEQLMRAAD
                     IFVSGSHREGSGYSLIEALACGLPPAVTDIPSFRSLTGHGAVGALWPTGDAAALCDAL
                     LAIAAQPDAARRAAARRHFEYELSFEAVGRKLAVAYQDLQHRMARAS"
     gene            complement(1879..3393)
                     /locus_tag="VGE92_13335"
     CDS             complement(1879..3393)
                     /locus_tag="VGE92_13335"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY1314858.1"
                     /translation="MTPTKRVLLINPTITSRRSARFPLAVLALSAALDGKYASSIIDG
                     NKDRDFIATTLRTLASGHIGAVGLSVMGGPQLLSAIAVSRAIRARYPATPIIWGGHFP
                     TIAAESALNSAYVDYAIRGQGEATLAELLDAILGGAESPLEPIAGLSWRSAAHIVHNS
                     NRPFSAASLARELPYERLGNPRQYLARTYLGRRTTGYQAALGCRFRCTFCGVAGMFRG
                     KTALPAAARLERDLQFLTSRLGVDAVQFYDHNFFDRETDTAPLLEVLAKFQLPWWCFA
                     RSDALLNLSDRSWAWVRKSRLRMAYIGAESPSDWLLHDVRKGTRTDQTLAAVEKCRGN
                     GVIPELSFMLAPPQDPEGETERTFEFIRHIKRVHPKTEIMLYIYAPLPPPPASENPQV
                     ERAVAELRDCDGGRIVFPSTADGWAEPQWISYWCHTDVPWLTPRIRNRIRDFTTVLGC
                     RFPTITDIRSPYWGKAALRTLASWRYRHQRYERPWELNFSKRFIRLWDPRVSGL"
     gene            complement(3390..>4638)
                     /locus_tag="VGE92_13340"
     CDS             complement(3390..>4638)
                     /locus_tag="VGE92_13340"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY1314859.1"
                     /translation="AVCLGITVLTGAPIHDALQVSRAAKRARPSLTVVWGGWHPSMFA
                     RECLTERSVDVTVRGQGEETFAEIVQRLAAGRSLSGCAGCTVRLEDGTIQENPPRPLA
                     QVEKFRAHDYGLIPVERYFDLKGKRQLDFISSQGCNFRCAFCSDPFVYGRKWVGLEPV
                     RMALRLKELWNRYHFDDLNFQDETFFTKADRVRALAEQIIESGMKISWAATMRADQGI
                     RLPDEVWQLCKRSGLRRLLVGVESGSDEVLKRIRKDIKIEQVYVTADKMLKLGVAGHF
                     PFIVGFPDESDADIQASLDCAKRLRSMSPDFLTPIYYFKPYPGSALVIEAVARGFRLP
                     ETLQAWAEFDYVAGDPGPWVSAEKFARIERFKFFHELAWKRVSRGKRLLQKLARYRCD
                     RDDYRWPVEMLLTRWLVPVQRLS"
BASE COUNT          925 a         1437 c         1482 g          794 t
ORIGIN      
        1 gcggataaag aattcgtagg ggaccgccgt gcccgagcgc agcgctttgt cgagcactcg
       61 aacgctatcg cggaaggaag tcgcggggtc tccatagtgt cgcgcgtgac gctgcaccac
      121 cgtcagcggt tgcggaacgg cgtcgatcgc gctgcgcgcc gccaaccgca gccagagatc
      181 gtagtcctcg cacataacca gctcttcgtc gaagcctccc acctcgtcga gcaacgttcg
      241 ggctaccacc acgctcggca gcgctatcac ggtctcggtg cgcagcaggg gaccgagaat
      301 ccaaccgtcc gcggccggca tttcccgcct cgactgcttg gccgcgttca ccaggacaaa
      361 ttgtgtgtga ctccaaccgc agtcgccgcg ggcgcgcagc gaggcgattt gcatgctgag
      421 cttgtcaggc atccaaacgt cgtcggagtc cataaacgcg acatactcgc cgttggcctg
      481 gcgcagcgcg cggttgcgcg ccaccgccgg tttgcctgag tgcggcagtc ggagcaactg
      541 cacgcgcggc gggtccacca gcgtctgcaa ataggcgcac gtttccgtgt ccgagccatc
      601 atcggcgatc agcaattccc aattctcgta gctctgcgcc aaaactgagg ccacggcctc
      661 tggcaggtat tgaagacgat tgaacgtcgg taaaacgatg gagacgagcg gcgaatttct
      721 catgaggctc gagccatgcg atgctgcaaa tcttgatagg cgacggccag ttttcgaccc
      781 accgcctcga aggacaactc atactcgaaa tgccggcggg cggctgcgcg ccgtgccgcg
      841 tcgggctgcg cagcaatcgc aagcagggcg tcgcataacg ctgccgcatc gcctgtcggc
      901 catagcgctc ccactgcacc gtggcccgtg agcgagcgaa aggaggggat gtcggtgacc
      961 gctggcggca atccgcacgc gagcgcttcg atcaaggaat agccgctgcc ctcgcggtgg
     1021 ctgcccgaca cgaaaatatc ggcggcacgc atcagttgct cgatctcgcc gtgggagact
     1081 cgtcccaaca aatggacccg gccgcacaac aggggatcgc cgctgatgcg ccctcgaacc
     1141 gccggcagca acgccgtgtc gccatagcag caatagagct gcaaatgcgg caaccggcgc
     1201 gccgtcttgg ctattccatc gaggactacg agcggatcct tgttggcgtc caaatgaccc
     1261 acccagagga tcaaaggatc gcccgaaacc ctcgaggagc cgcgcgcctt gaccttgtcg
     1321 cccggagtga aacggctgct ggattccgga attgcgtaca acttcgcgcg ggcgcgaagc
     1381 aggccggccg cggcgaatgg tcgggcctgc tccacggagc agaatgcaat tcctgcggca
     1441 gccgagaagc cgcgccgcca caacgcgcgc cgccacggcc gccacgcccg cggcggcctg
     1501 ctggcatgat cctgcaaaag caacggaatg ccgggcgcag cggccgtcag cgacaaaaca
     1561 tccgctgcga aatccagacc gtgcacgtgg agaacatcgg gggagatgct atgcaacagt
     1621 tcgtgaaacc ggccggcaag ccgtgcgcgc ccgccgtata ccgcgcggcc gaaggggaga
     1681 aaatggtagt cgacgccagc gcgccgtaaa tcctgcgatt gaccgcaggc ttgtatgacc
     1741 gacactcgaa cgccggcgcg gctcgcacaa tcggcgacat cgccgagact cggccaatct
     1801 tgcaagagct gctcgggtgc gcgcgcctgc ggatcgcaga aaaagctgat ctgagcgacg
     1861 cgcattggcg ggctagcgct acaggcccga gacgcgcggg tcccacaggc gaatgaagcg
     1921 cttggagaaa ttcagctccc aggggcgttc gtagcgttgg tggcgatagc gccaagatgc
     1981 gagggtgcgc agcgccgctt ttccccaata aggcgaacga atgtcggtga tggttgggaa
     2041 tctgcagccg agcaccgtgg tgaagtcgcg gatgcgattg cgtatccgcg gcgtgagcca
     2101 aggcacatcg gtatggcacc agtacgagat ccattgcggc tcagcccagc cgtcggcggt
     2161 gctcggaaac acgatgcggc caccgtcgca gtcgcgaagc tcggcgacgg cgcgctccac
     2221 ctgggggttc tcgctggcgg gcggcggcgg aagcggtgca tagatgtaga gcataatttc
     2281 ggtttttggg tgcacgcgct tgatgtggcg aatgaactcg aaggtgcgtt cggtctcgcc
     2341 ctcgggatct tgaggcggcg ccaacatgaa agacagttcc ggtatgacgc cgttgccgcg
     2401 gcatttctcc acggccgcga gcgtttggtc ggtacgggtt cccttgcgaa cgtcatgcag
     2461 tagccaatcg ctcggggatt ccgcgccaat gtacgccatg cgcaagcggc tcttgcgaac
     2521 ccacgcccag gagcgatccg aaagattcag cagcgcgtcg gatcgcgcga agcaccacca
     2581 gggcagctgg aatttcgcca acacctcgag cagcggtgcc gtgtccgtct cacggtcaaa
     2641 aaagttatgg tcgtagaatt ggaccgcatc cacgcccagg cgagaggtca agaattgcaa
     2701 atcgcgctcc aggcgcgcgg cggccggcag cgcggttttg ccgcggaaca tgcccgcgac
     2761 tccgcaaaac gtacagcgga agcggcaccc gagggcggcc tggtagcccg tggtacgtcg
     2821 gccgagataa gtgcgggcta gatactgtcg gggattcccg agccgttcgt aggggagttc
     2881 tcgagcgagg cttgccgccg agaacggccg attgctgttg tgaactatgt gcgctgctga
     2941 tcgccacgac aaaccggcaa ttggttcaag cggcgattcg gcgccaccta agattgcatc
     3001 cagaagttcg gcgagcgttg cctcgccctg gccgcggatg gcgtaatcga cataggctga
     3061 attgagcgcg gattcggcag cgatggtcgg aaaatgaccg ccccaaatga tgggagtcgc
     3121 tggatatcgc gcgcgaatcg cccgcgagac ggcgatggcg ctgagcagtt gcggacctcc
     3181 cataacgctc agcccgacgg cgccgatgtg gcccgacgcg agggtgcgca gcgtggtagc
     3241 gatgaagtca cggtccttgt tgccgtcgat gatgctcgac gcatactttc cgtccaatgc
     3301 cgccgataag gcgagtaccg cgaggggaaa acgcgcgctg cggcgcgagg taatcgtcgg
     3361 gttgatcaac aagacgcgtt tggtcggcgt catgacaatc tctgaaccgg caccagccag
     3421 cgggtcagga gcatctccac cggccagcgg tagtcgtctc tgtcgcagcg atagcgagcg
     3481 agcttttgca ggagccgttt accccgcgag acgcgcttcc atgccaattc atggaagaac
     3541 ttgaagcgct cgatgcgcgc gaacttctcg gcggacaccc agggtcccgg gtcacccgcc
     3601 acatagtcga attcagccca ggcttgcagc gtttcgggca ggcgaaagcc gcgggccacg
     3661 gcttcgatga ccagcgcgct gcctgggtag ggcttgaaat agtagatcgg cgtcaagaaa
     3721 tccgggctca tcgagcgtag ccgcttggcg caatcgaggc tggcttgaat gtccgcgtcg
     3781 ctttcgtccg ggaagccgac gatgaatgga aagtgtccgg ccacgccgag cttcagcatc
     3841 ttgtccgccg tcacatacac ctgctcgatc ttgatgtctt tgcgtatgcg tttcagcacc
     3901 tcatcggagc cggattcgac gcccaccagc aagcggcgca agcccgatcg tttgcatagc
     3961 tgccatacct cgtcgggcag gcggatgcct tggtcggcgc gcatggtcgc cgcccagctg
     4021 atcttcatcc cggattcgat gatctgttcg gcgagtgccc gaacgcgatc ggccttcgta
     4081 aagaaagtct cgtcctggaa atttaggtcg tcgaaatgat agcgattcca caattccttg
     4141 agccgcagcg ccatgcgcac gggttcgaga ccgacccact tgcgcccata gacgaatgga
     4201 tccgagcaaa acgcgcagcg aaaattgcag ccttgagatg aaatgaagtc cagctgccgt
     4261 ttgcccttga ggtcgaagta acgctccact ggaatgagcc catagtcgtg cgcacggaac
     4321 ttttcgactt gcgccagcgg ccgcggcgga ttctcctgaa tggtgccgtc ctcgagtctg
     4381 accgtgcatc ctgcacagcc ggagagcgag cggcccgcgg cgagccgctg cacgatttcc
     4441 gcaaacgtct cctcgccctg gccgcgaacg gtgacatcga ccgaacgttc ggtcagacat
     4501 tcgcgcgcga acatggaggg atgccaacct ccccatacga cggtcagcga tggccgtgcc
     4561 cgcttcgccg cgcgtgaaac ttgcagcgca tcgtggatag gcgcgccggt gagcacggtg
     4621 atcccgagac acacggcg
//
LOCUS       DASWZT010000466         3583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_13606, whole genome shotgun sequence.
ACCESSION   DASWZT010000466 DASWZT010000000
VERSION     DASWZT010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13606"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VGE92_13345"
     CDS             complement(<1..442)
                     /locus_tag="VGE92_13345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEY1314860.1"
                     /translation="MTASDTTAKRRWLALFDLDGTLTWRDTLLPFLLSFLRHHPWRIC
                     GLWRLPAALFNYATRDRDRGELKSRVIRMLMGGATRGAVDRCAQSFVDSLQPRHRFRR
                     VALAMLETHRAAGDLLVLLSASPDLYVPRIGRSLGFDRTLCTEIE"
     gene            633..1448
                     /locus_tag="VGE92_13350"
     CDS             633..1448
                     /locus_tag="VGE92_13350"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY1314861.1"
                     /translation="MLIRLGYELAYEFPQATPMILNLNVHFSRTADWVRPDTIVTHPS
                     VPLGMYRDDFGNWCTRLIAPPGQFLVTTDALINDTGLAEPSAPHAFEHPVPSLPDGTL
                     VFLLPSRYCETELLAPTAWGLFGGMMPGWSRVQAICDYVHDHIAFDYQCARPTKTAWE
                     TYNERRGVCRDYAHLAITLCRCLNIPARYCTGYLGDIGVPPVDAPMDFAGWFEAFLGG
                     EWHVFDPRNNQRRIGRVLIARGRDAADVAISMTFGPNVLQRFRVWTDEVRPNS"
     gene            1492..2898
                     /locus_tag="VGE92_13355"
     CDS             1492..2898
                     /locus_tag="VGE92_13355"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY1314862.1"
                     /translation="MSQWVLEKFKTAWLGCLGLAALGAGLAPAQAATPVAVAGFSFIK
                     SLGGIDEYRLDANGLTVLLVPDHSAPVVTFQVTYQVGSRNEVTGATGATHILEHMMFK
                     GSENFNDPKGNSVKQFLERVGGQFNASTSFDRTNYFATIGRESLEGYVAIEADRMRHL
                     WLREADRQSEMTVVRNEYERGKNDPDSVLLEEVTAAAYVALPYHHPTIGWKSDIEHVP
                     IGRLREFYDTFYWPNNATVTAIGDIEPAAALGLIKKYYGVYPHSPAAIPNIYTEEPAQ
                     SGERRVTVKRPGELGTVIIAHKVPNGRDADQPALEMLDAILSSGKNARLYRALVDQGL
                     ALNAGAGTDLHRDLSLHTLYAVLAPGAAHAQVEKALLAEIGKIKSDGVTAAEVARVKQ
                     QYIAADAYKRDGTAAVAGEINEWIGVGDWTLYVTFPQKVQQVTPADVQRVAKQYLNDD
                     QSTTGWFIPKPPAAEKGS"
     gene            2898..>3583
                     /locus_tag="VGE92_13360"
     CDS             2898..>3583
                     /locus_tag="VGE92_13360"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEY1314863.1"
                     /translation="MRQLVLAALILASGSAAANVASQSHRSQVAGIDLITYRTEVKDV
                     VVILGSLPAGDAMAEPGNIAIPTLTGMMLDRGTKALDKFAIAEQLDNVGAEIAFSVGT
                     QSLEIRAKCLKKDLPLVIGLIATELRTPALQLAEFNKAKQQFIGALEASAQNTEARAQ
                     EAFGRAIFPQGHPNRPHAVSEYEAAAKSATLDELKAFHTKFYGPAHLTLVVVGDVAAP
                     ETAAEIGKAFA"
BASE COUNT          726 a         1083 c         1151 g          623 t
ORIGIN      
        1 actctatctc ggtgcacagc gtgcggtcga accccaggga acggccgatg cgcggcacat
       61 acaaatcggg gctcgccgac agcaacacca gaagatcgcc ggcggcgcga tgcgtctcga
      121 gcatggcgag cgccacgcgg cgaaagcggt gccgcggctg cagggagtcg acgaaggact
      181 gggcacagcg atccaccgcg ccgcgcgtgg caccccccat gagcatgcgg atgacccgac
      241 tcttcaattc gccgcggtcg cgatcgcgcg tcgcataatt gaagagcgcg gccggcaatc
      301 gccacaggcc gcagatgcgc caggggtggt ggcgcaggaa actcagcagg aagggcagca
      361 gcgtatcgcg ccaggtgagc gtgccgtcta aatcgaagag cgcgagccag cgacgttttg
      421 cggtagtgtc agaggcagtc atacataaat aatgttgatt aacagatgct taaggcgatt
      481 atctcgaaaa tttcttgatt cggctgccgc cttgcgaatg ctgcgtagga tgctgactac
      541 gattcgtgag gccctgcgcc gctgtggctg gaacgccgag catggcactt taaggagtag
      601 attgggatac aaaatctagg gtggtgacct gcatgctgat acgactgggc tacgaattag
      661 catatgagtt tccccaagcg acgccgatga ttttgaatct gaatgtgcat tttagccgca
      721 ccgctgactg ggtgcggccg gataccatcg tcactcaccc gagcgtgccg ctcggcatgt
      781 accgcgacga tttcggaaac tggtgcaccc gtttgatcgc gccgcccggc caattcctgg
      841 tgaccaccga cgccctgatc aacgacacgg gcctcgcgga accgagcgct ccacacgcct
      901 tcgagcaccc cgtcccatca ttgccggacg gaaccttggt gtttctgctg ccgagccgtt
      961 actgcgaaac ggagttgctg gcgccgacgg cgtggggtct gttcggcggc atgatgccgg
     1021 gctggagccg ggtgcaggcg atttgtgact atgtgcatga tcacatcgcc ttcgactatc
     1081 agtgcgcgcg cccgacaaag accgcgtggg aaacctataa cgaacggcgc ggggtgtgcc
     1141 gcgattacgc gcatctggcg atcacgttgt gccgatgtct gaatattccg gcgcgttatt
     1201 gcaccgggta tctcggggac atcggcgtgc cgcccgtgga tgcgcccatg gacttcgccg
     1261 gctggttcga agcctttctc ggcggcgagt ggcatgtctt cgacccgcgc aacaaccagc
     1321 gccgcatcgg gcgggtgctg atcgcgcgcg gccgcgatgc cgccgatgtc gcgatcagca
     1381 tgaccttcgg gccgaacgtc ctgcagcgtt tccgcgtgtg gaccgacgaa gtgcggccga
     1441 actcataagc atctacaatg cgcgatcgtt agcggcgggg cgcggatgac tatgagtcaa
     1501 tgggtgcttg aaaaattcaa aacggcgtgg ctcggctgct tgggcctcgc tgcgctcggc
     1561 gcgggattag cgcccgcgca ggcggcaaca ccggtcgcgg tggcgggctt cagcttcatc
     1621 aaatccctgg gcgggatcga cgagtatcgg ctcgatgcca acggccttac cgtgctgttg
     1681 gtgcccgatc attcggcgcc ggtcgtgaca tttcaagtga cctaccaggt tggttcgcgc
     1741 aacgaggtga ccggtgctac gggagccacg catatcctcg agcacatgat gttcaagggc
     1801 agcgagaact tcaacgatcc gaagggcaac agcgtcaagc aattcttaga gcgcgtcggc
     1861 ggccaattca acgccagcac ctcgttcgac cgcaccaact atttcgcgac catcggccgc
     1921 gagagcctgg agggctacgt ggcgatcgag gccgaccgca tgcgccatct gtggctgcgc
     1981 gaagccgacc ggcagtccga aatgacggtg gtgcgcaatg aatacgaacg cggcaagaac
     2041 gacccggaca gtgtgctgct cgaggaggtg accgcggcag cctacgtggc attgccctac
     2101 caccatccga ccatcggctg gaagagcgac atcgaacatg ttccgatcgg cagattgcgc
     2161 gagttctacg acacattcta ttggcccaac aacgcgaccg tcaccgcgat cggcgacatc
     2221 gaaccggccg ccgcattggg cctgatcaag aaatactacg gcgtctaccc gcattcccct
     2281 gcagccatac ccaatatcta taccgaggaa ccggcgcaga gcggcgagcg ccgcgtcacc
     2341 gtcaagcgtc ccggcgaact cggcaccgtg atcatcgcgc acaaggttcc gaacggccgg
     2401 gacgccgacc agccggcgct cgaaatgctc gatgccattt tgagttcggg caagaacgcg
     2461 cgcttgtacc gcgcgctggt cgatcaaggt ttagccttga acgccggcgc cggcaccgat
     2521 ctgcaccgcg atttgtcgct gcacacgcta tatgcggtgc tcgcacccgg cgcggcgcac
     2581 gcgcaagtgg agaaggcgtt gctggcggaa atcggcaaaa tcaagagcga cggcgtgacc
     2641 gcagcggaag tggcgcgggt gaaacagcag tacattgcgg ccgatgccta caagcgcgat
     2701 ggaaccgccg cggtggccgg cgagatcaac gagtggatcg gggtgggcga ctggactttg
     2761 tacgtcacct tcccgcagaa agtccagcag gtgactcccg cggacgtaca gcgcgtcgcg
     2821 aaacagtatc tcaacgacga tcagagcacg acgggttggt tcattcccaa gccgccggcg
     2881 gcggagaagg gctcgtgatg cggcaacttg tgttggcggc gctgattctt gcaagcggca
     2941 gcgcggccgc gaatgtcgcg agccagagcc accgttcgca ggtggcggga atcgacttga
     3001 tcacgtaccg caccgaggtc aaagacgtgg tcgtgatcct agggtcgttg ccggccggcg
     3061 atgcgatggc cgagcccggg aacattgcca ttccgacctt gaccggcatg atgctcgacc
     3121 gtggcacgaa agcgctcgac aaatttgcga tcgccgagca gctcgacaac gtcggcgccg
     3181 aaatcgcctt cagtgtcggc acgcagtcgc tcgagattcg ggcgaagtgc ctgaagaaag
     3241 acctgccgct cgtgatcgga ctcatcgcca cggagctgcg tacgccggcc ctgcagctgg
     3301 cggagttcaa caaggcaaag cagcaattca tcggcgccct ggaagcctcg gcgcaaaata
     3361 ccgaggcgcg cgcgcaggag gccttcggcc gggcgatttt cccgcagggc catccaaatc
     3421 ggccgcatgc ggtgagtgaa tacgaggccg ccgccaagtc ggcgacgctc gatgagctca
     3481 aggctttcca tacaaaattc tacggtcccg cgcatttgac gctggtcgtg gtgggcgatg
     3541 tagccgcgcc ggaaacggca gccgagatcg gcaaggcatt cgc
//
LOCUS       DASWZT010000467         1733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_42666, whole genome shotgun sequence.
ACCESSION   DASWZT010000467 DASWZT010000000
VERSION     DASWZT010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42666"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1510
                     /locus_tag="VGE92_13365"
     CDS             167..1510
                     /locus_tag="VGE92_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEY1314864.1"
                     /translation="MTNDTILPFSFPAVHTKKVTAAFDGGRLTSNGGVMLLAMAERRL
                     GLADNLARVFPDRRDPTRVVHSLVDMFRARMFAICCGYEDADDLDHLRSDPAFKLACG
                     RLPDTGRDLCSQPTLSRLENAPRLRDVIRLTYTLVDAWMDSYPCEPASVTLDIDDTCD
                     VVHGHQQLSLFNAHYDERCFLPIHVYDTEKSRPVAVVLRPGKTPGGIEVRAHLRRLVR
                     HIRTRWHKTRITFRGDGHYARPEAMTWCENNGIDYIFGLSGTKPLARKLDEVADDIRT
                     RRAIGNLAVLRGYTETRHKAKSWDRERRTVARIEATMLGLDIRFVVTSLDVGSAEWIY
                     DSLYCARGQAENLIKLHKTQLASDRTSCRSALANQVRLVLHTAAYWLMLTVRDAIPKV
                     RELATAEFATLRLRLLKIAARVVETTSRIRLAFAAACPEADLFRSLPGALLPLGP"
BASE COUNT          316 a          573 c          511 g          333 t
ORIGIN      
        1 aggtaaacct gagtcaatcc tcgccaatac cagcctgtca ctagcccatt ttattcgcca
       61 gagggtgcct gagaggctgg cgagcgtggt gtaaagcgct gatgcggcgt aggattcggt
      121 tgcgaagcca accccatcac cgtcaaccgc gaacgccacg cccgccatga ccaatgatac
      181 gattctgcca ttctcatttc cagccgttca caccaagaaa gtcacagctg ccttcgatgg
      241 cggtcgcctg acctcgaacg ggggcgtgat gcttctggcg atggcggagc ggcgtctcgg
      301 tttggccgac aatttggccc gggtgttccc ggatcggcgc gatccgacgc gggttgtgca
      361 cagcctggtc gatatgttcc gtgcccgcat gttcgcgatc tgctgcggct atgaggacgc
      421 cgacgacctc gatcatctgc ggtccgatcc tgcgttcaag ctggcctgcg gtcgcctgcc
      481 ggacaccggc cgggatttgt gttcccagcc gaccctgtcg cggctggaga atgcgccgcg
      541 actgcgcgac gtgatccgcc tgacctacac cttggtcgac gcatggatgg atagctatcc
      601 gtgcgagccg gcatccgtca cgctcgacat cgacgacacc tgcgacgtcg tccacggcca
      661 tcagcagctc tcgctgttca acgctcatta tgacgaacgc tgcttcctgc cgatccacgt
      721 ctatgacacg gagaagagcc ggcccgtggc ggtcgtgctg cggcccggca agacgccggg
      781 cggcatcgag gtccgtgccc atcttcgccg cctggtacgg cacatccgca cgcggtggca
      841 caagacgcga attacgttcc gtggcgacgg gcactatgct cggccggagg cgatgacgtg
      901 gtgcgagaac aatggcatcg actacatctt cggtctgtcc ggtaccaagc ctctcgccag
      961 aaaactcgac gaggtcgctg acgacatccg cacgcgacgc gccatcggga acctggctgt
     1021 tctgcgcggc tataccgaga cgcgccacaa ggcaaagtcc tgggatcgcg aacggcgcac
     1081 cgtcgcccgt attgaggcga cgatgctcgg cctcgatatc cgcttcgtcg tcaccagcct
     1141 cgatgttggc tcggccgagt ggatctacga cagcctgtat tgcgcgcgcg gccaagccga
     1201 aaatctgatc aagctgcaca agacgcagct cgcctccgat cgcaccagct gccgttcggc
     1261 gctcgccaat caagtccgcc ttgtgctcca tacggccgct tattggctga tgctgaccgt
     1321 gcgcgacgcc attcccaaag tccgggaatt ggccactgcc gagttcgcga cgctgcgtct
     1381 tcgccttttg aaaatcgccg cccgggtcgt cgagaccacg agccgcattc gccttgcgtt
     1441 cgccgcggca tgtcccgaag ccgacctctt ccgcagcttg cccggcgcgc tgctgccgct
     1501 cggtccctga acagcggggg cgcgtgcccc gttcgcccaa tccatccctc aagcgcgttg
     1561 caatgtacgg gtcgtcaggc ggctaaaagc cgaaggcaat cctgtgcgcc tcgtcagagg
     1621 aggtgcgcgg acacaccaat cggaccaaaa aaccgcgctc tcacgaatag gacgggctag
     1681 gttggagtca atctcggtag atttctttag atacgccgca ccttcttcta aac
//
LOCUS       DASWZT010000468         4441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_56969, whole genome shotgun sequence.
ACCESSION   DASWZT010000468 DASWZT010000000
VERSION     DASWZT010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56969"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VGE92_13370"
     CDS             complement(<1..436)
                     /locus_tag="VGE92_13370"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEY1314865.1"
                     /translation="MNAVHLERSLPSSWYRTPEVFRAEKERIFCREWIAACREEELPN
                     AGDSMVLDVLGESILLVRNRDGELRGFYNVCRHRGSRLCRSAAESAALTVTLPGGITA
                     GRIVCPYHQWSYDFNGALVAAPHLTTASGFAKQDYSLYPVGVD"
     gene            complement(433..2493)
                     /locus_tag="VGE92_13375"
     CDS             complement(433..2493)
                     /locus_tag="VGE92_13375"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1314866.1"
                     /translation="MARNPRFDVLFEPVRIGPVTAPNRFYQVPHASGMTNALPQVRAS
                     FRETKAEGGWGVVCTGAVSIHPSSDDSPLPFAILWDDKDIRCHALMTDAVHRHGALAG
                     IELWHGGACTLNRTSRIAPLSPSGMPWLSTHSGFMGNLRPRAMDTADIQDLLRWQAAG
                     ARRAMQAGFDIVYVYAGMGYLPYEFLLPEYNRRSDAYGGSIENRVRLVRELLAVTRDA
                     VGGKCAVALRISLEELRGRPGKNAASEAHEVVALLADIPDLWDVKMDSSPTDCAPSRF
                     SPEGSHEPIVNFVKQLTHRPVVGVGRFTSPDVMVSQVRRGILDLIGGARPSIADPFLP
                     KKIDEGREHEIRECIGCNICISSWHDGVPVRCTQNPTAGEEWRRGWHPERFAPPASDS
                     SVLIVGGGPAGLECATTLARRGYTVTLADSAQEFGGRLRFETALPGLKTWNRVANWRL
                     GQLRERPNVNLYPGSALGVEDILGLEHRHVVLATGARWTKMQYSTLEFAVGELDGPAV
                     YTPDDLAAGVIPQAPVVVFDFDNYYLAGAIAEQLAKSVGDVSYVTPAGNASAWTFMTN
                     ELPLVHRALAKAQVAIHTLQRVSAFDGQAATLADIYSGSERRLACRSLVIVGVRAPRD
                     EIYEALIARRSDLESAGIDSVTRIGDALAPGAIVHAVHSGHRYAREFDTIPGTSPYAR
                     DYPL"
     gene            complement(2623..3096)
                     /locus_tag="VGE92_13380"
     CDS             complement(2623..3096)
                     /locus_tag="VGE92_13380"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HEY1314867.1"
                     /translation="MAPYGTLLASLLAGACATYPQIQEGLWDVRAQSTENPGAKKTEF
                     GYKLCRDHAYDKQTDSLVKNNKDCKTRLESAGANRYTAASRCTVSATVIVSKGLSIYQ
                     SGNSIHSETVATYTPPLYGKSDETLILDQKFLGSCPAGMKPGDRLLPDGRVAHPR"
     gene            complement(3123..4232)
                     /gene="ald"
                     /locus_tag="VGE92_13385"
     CDS             complement(3123..4232)
                     /gene="ald"
                     /locus_tag="VGE92_13385"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEY1314868.1"
                     /translation="MKIGVPKEIKIHEYRVGLVPAGVRELVASGHQVLVQSGAGAGIG
                     FEDAHYKAAGAVIAQSAPDIFASVDLVVKVKEPQLAECKQLRSGQTLFTYLHLAADRE
                     QTQALLASGATAIAYETVTAPDGSLPLLTPMSEVAGRMSVQVGANSLQKANGGFGVLL
                     GGVPGVAPAKVVILGGGVSGTHAAEMAIGLRADVTVVDRSVKRLRELSSIFGSQLKTV
                     YSTAHAIEELVRDADLVIGAVLIAGAAAPKLVTRAMVKTMKPGAVLVDIAIDQGGCFE
                     TSRPTTHAEPTYVLDGVIHYCVTNMPGAVPRTSTFALTNATLPYVRALADLGWRQAIV
                     NDPGLARGLNVHAGQLTHEAVASALGLDYRAFKAA"
BASE COUNT          836 a         1438 c         1388 g          779 t
ORIGIN      
        1 tatcgacgcc gaccggatac agagagtaat cctgcttcgc gaagccgctg gccgtggtca
       61 gatgcggggc cgcgaccagc gcgccgttaa agtcatagga ccactgatgg tacgggcata
      121 cgatccgtcc cgcagtgatc ccgcccggca gcgtgacggt gagcgcggcg ctctcggcag
      181 cgctgcggca caggcgcgac ccgcgatgcc ggcagacgtt gtagaaaccg cgtaattcac
      241 cgtcgcgatt gcgaaccaac aaaatgctct cgccgaggac atcgagcacc atggaatcgc
      301 cggcattggg caattcctcc tcgcggcacg ccgcaatcca ttcgcggcag aaaatgcgct
      361 ccttttcggc gcgaaacacc tcgggagtac ggtaccagct ggagggcagg ctgcgttcca
      421 aatggacggc attcataacg gataatcccg tgcgtagggt gaggtccccg gtatcgtatc
      481 gaactcgcgc gcgtagcgat gtccgctgtg aaccgcgtgc acgatcgcac ccggcgcgag
      541 cgcatcgcca atgcgcgtaa ccgagtcgat tccagcactc tcgaggtcgg aacggcgcgc
      601 gatgagcgcc tcatagatct catcgcgtgg cgcccggacc ccgacaatga ccaatgagcg
      661 gcaggcaagc cgccgctcgc tgccgctgta tatatccgcg agcgtcgccg cttggccgtc
      721 aaaagcgctc actcgctgca acgtatgtat ggcaacctgc gctttggcga gcgcgcgatg
      781 cacgaggggc agctcgttgg tcatgaacgt ccacgccgac gcgttgccgg cgggcgtgac
      841 gtagctgacg tcgccgaccg acttcgccag ctgttcggca atggcgcccg ccaggtagta
      901 gttgtcaaaa tcgaaaacca ccacgggggc ctgcgggata acgccggcgg caaggtcgtc
      961 gggcgtataa acggcaggcc cgtcgagttc gccgacggcg aactccaagg tcgaatactg
     1021 catcttggtc cagcgcgcgc cggtcgcgag cacgacatgg cgatgctcga gtccgagaat
     1081 atcctcgacg ccgagcgcgc tacccgggta gaggttgacg ttcggtcgtt cgcgcaactg
     1141 ccccaagcgc cagttggcga cgcggttcca cgtcttgagt cccggaagag cggtttcgaa
     1201 gcgcagccgc ccgccgaatt cctgcgccga atctgcgagg gtcaccgtgt aaccgcggcg
     1261 ggccaacgtc gtggcgcact cgagccccgc agggccgccg ccgacgatca agacggacga
     1321 gtcgcttgcg ggcggtgcaa atcgctcggg gtgccaaccg cggcgccatt cctcgccggc
     1381 cgttggattc tgcgtgcaac gcaccggcac gccatcgtgc cagctcgaga tgcagatgtt
     1441 gcagccgatg cattcgcgaa tttcgtgctc gcgcccttca tcgatcttct tcggcaggaa
     1501 cggatccgca atcgagggcc gggcgccgcc gatcaagtcg agaatgccgc gacgcacctg
     1561 cgagaccatc acatccggag aggtgaagcg gccgacgccc accaccggcc ggtgagtcaa
     1621 ctgcttgacg aaatttacga tcggctcgtg gctgccctcc ggagaaaaac gcgacggcgc
     1681 gcaatcggtc ggactcgaat ccatcttgac gtcccagaga tcaggtatgt cggcaagcag
     1741 ggccacgact tcatgcgcct ccgaggccgc attcttgcca ggtctgccgc gcagttcctc
     1801 caggctgatg cgcagcgcca ccgcgcactt gccgccgacg gcatcgcggg tcacagccag
     1861 gagttcacgc accagacgca cgcgattctc gatcgaaccg ccgtaggcgt cgctgcggcg
     1921 attgtattcg ggcaacagga actcatacgg cagatagccc attccggcgt agacgtacac
     1981 gatgtcgaat ccagcctgca tggcgcgccg cgcgccggcc gcctgccagc gcaacaaatc
     2041 ctgtatgtcc gccgtgtcca tggctcgcgg acgcagattc cccatgaagc ccgaatgcgt
     2101 cgacaaccag ggcatgcccg acggcgacag aggcgcgatg cggctggtgc ggttcaacgt
     2161 gcaggcgccg ccgtgccaaa gttcgattcc cgccaacgca ccgtggcggt gtaccgcgtc
     2221 ggtcatcagg gcgtggcagc ggatatcctt gtcgtcccag agaatcgcga acggcagcgg
     2281 cgagtcatcc gagctcggat gaatcgagac ggcaccggtg cacaccacgc cccagccgcc
     2341 ttcggctttc gtttcgcgga agctcgcgcg cacttgcggt agcgcattcg tcatgccgct
     2401 ggcgtgcggg acctgataaa aacggttcgg cgccgtgacc ggcccgatgc gcaccggctc
     2461 gaacaaaacg tcaaagcgtg gattgcgggc catggcggat cccttgcctt atagggtttt
     2521 gcgggcgcct agagtaattg atcggtcgta cagtagcaac cgggccgacg cggcatggtg
     2581 cattgcatgg ccggacaatg cgcgtgcgcc gtcgcgtcgc ggttacctgg ggtgagccac
     2641 ccggccatcg ggcaataagc gatcaccggg tttcattccg gccgggcaac tgccgagaaa
     2701 tttctgatcc agaatcagtg tttcgtcgct cttgccgtag agcggcggcg tgtacgtggc
     2761 aaccgtctcc gaatgaatgg aattgccgct ctgataaatc gatagcccct tcgaaacgat
     2821 gacggtggcg gacacggtgc agcgcgatgc ggccgtatag cggtttgcac cggcagattc
     2881 gagtctggtc ttgcaatcct tgttattctt caccaaggaa tccgtttgct tatcgtaggc
     2941 atggtcgcgg cagagcttgt agccgaactc ggttttcttg gcgccgggat tctccgtgct
     3001 ttgcgcccgg acgtcccaca gcccttcctg gatttgggga tacgtcgcgc acgcgcccgc
     3061 cagcagcgac gcgagcagcg ttccgtacgg ggccatgcgc ctcacgctac agttccgccg
     3121 cctcaggcgg ctttgaaggc gcgataatcc agcccaagcg cgctcgccac cgcctcgtga
     3181 gtcaattgcc cggcatgaac attgagcccg cgcgccaaac ccgggtcatt gacgatggct
     3241 tgacgccaac cgagatcggc cagcgcccgc acatacggca gggtggcatt ggtcagcgcg
     3301 aacgtggacg tgcgcggcac ggcgcccggc atgttggtga cgcagtagtg aatcacgccg
     3361 tccagaacat aggttggttc ggcatgcgtc gtggggcggc tggtctcgaa gcagccgccc
     3421 tgatcgatgg cgatatcgac cagcactgca cccggcttca tggtcttgac catcgcccgc
     3481 gtaaccaatt tcggcgcggc ggcgccggcg atgagcacgg cgccgatgac cagatccgca
     3541 tcgcgcacca gctcttcgat cgcatgcgcg gtcgagtaga cggtcttcag ttggctgccg
     3601 aaaattgacg acagttcccg cagtcgcttc accgagcgat cgaccacggt cacatcggcg
     3661 cgcaggccaa tggccatttc cgccgcatga gtgccggaca cgccgccgcc caggatcaca
     3721 accttggcgg gcgcgacgcc cggaactccg ccgagcagca cgccgaagcc gccattggcc
     3781 ttctgcaagc tgttggcgcc gacctgcacc gacatgcgcc ccgcgacttc gctcatcggc
     3841 gtgagcaaag gcaacgagcc atcgggcgcc gtcacggtct cgtacgcgat cgccgtcgcg
     3901 ccggacgcga gcaatgcctg cgtctgttca cggtcggcag ccaagtgcag ataagtaaac
     3961 aaggtctgcc cgctgcgcag ttgcttgcac tccgcgagct gcggctcctt gaccttcacg
     4021 acgagatcga cgctcgcgaa gatatccggg gcactttgcg cgatcaccgc gccggccgcc
     4081 ttatagtgcg catcctcgaa gccgatgccc gccccggcgc cgctctgcac caatacttga
     4141 tgtcccgaag ccacgagttc gcgaacgccc gccggcacca agccgactcg atactcgtga
     4201 atcttgatct ctttggggac gccgattttc atgatgagtg ctccgctaag acggggggtg
     4261 ggattcatag cgtacggcag ggcgccgccg gaaagttgca aaaactggtc ttcggaggcg
     4321 gtctgatggc agaatacagc gcactttgca tgtttcgcgc aagaaactgc cgcttttggc
     4381 ccggagcctc gcaatgaaat tggatcgcta cgatcgtgcc atcgtgcgca cactgcagct
     4441 c
//
LOCUS       DASWZT010000469        14848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_95845, whole genome shotgun sequence.
ACCESSION   DASWZT010000469 DASWZT010000000
VERSION     DASWZT010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14848
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95845"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1226)
                     /locus_tag="VGE92_13390"
     CDS             complement(183..1226)
                     /locus_tag="VGE92_13390"
                     /inference="COORDINATES: protein motif:HMM:NF023354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3445 domain-containing protein"
                     /protein_id="HEY1314869.1"
                     /translation="MSAVFKPDETFRGDFTYRNSPEAIRRFPLPFPEDAYMYSVNIEP
                     HLPGPIGSVTEFAFDVDEHYVAECRERAIVLAAEPLRYQALPHMLSSQWDCLELIMES
                     LARDYPAHFSLSKRGNRWHWINRPLQIDQQFTFGDTSSLPREPLDYITRQAQGDFVVM
                     DQREADLWTEAGMVTTQADWSLDFDIGMTFKEWHGPVPLAHELQVFDRALKFLLNLRL
                     GQPVRRLNWTMTVNPRLDTSPENYPFWGTDRATVTADNAGERVHLRVELQGLWRLPRS
                     NAIVFGIRCYLISLRELATVPKWARRVHRVLRDLHPALVDYKGLTRYRDAAVKWLAPY
                     DDGSPTTPGTGPE"
     gene            complement(1246..2208)
                     /locus_tag="VGE92_13395"
     CDS             complement(1246..2208)
                     /locus_tag="VGE92_13395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HEY1314870.1"
                     /translation="MSDAQTLAVEVIGIEQVTPLIKHFKLAPVGGGDLPAFSGGSHII
                     VVMQGGDRVHKNPYSLLSAPHELQTYEIGVRRMEESRGGSHFMHDAVAVGSRLEIAHP
                     VNLFGLDKIARKHVLIAGGIGITPFLAQLEDLHGGGVPYELHYSVRSPEHAAFLERLR
                     AREGERLHMYYDSEAQSIPFEAMLASQPLGTHVYVCGPAGMIKHVIATAHACGWPDSH
                     LHWEQFSAPPVGDAFEVFLARANLKVHVPPDQSLLESIEAAGVEVPYLCRGGVCGFCH
                     TRVLELDGELIHNDHFLSDADKAKGKSIMPCVSRARCRRLVLDL"
     gene            complement(2205..2834)
                     /locus_tag="VGE92_13400"
     CDS             complement(2205..2834)
                     /locus_tag="VGE92_13400"
                     /inference="COORDINATES: protein motif:HMM:NF041259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylamine monooxygenase subunit DmmA family
                     protein"
                     /protein_id="HEY1314871.1"
                     /translation="MLVTGIKSKPVYAPLQADARGRYHLMLGMGVGAAPLLRVIAEMR
                     GAAAASLENTRVLFVPDAAGGTGAGAPAGVEQFRDAGVDHADSYATTAALLEEFRHSL
                     ASSLMGTRLYVAGPESFIGLAMKIALEFNLNKDEIRAEECGTLARRVYCIHCRATTEN
                     VRTNIVRCVGCERWLLVRDHYSRRLAAYMGVMVDAEAPGELPPLVEVFL"
     gene            complement(2835..4520)
                     /locus_tag="VGE92_13405"
     CDS             complement(2835..4520)
                     /locus_tag="VGE92_13405"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY1314872.1"
                     /translation="MNSTADVAVVASGVKGAGLERGIDWTGAFWVASGVPPLVLFSIG
                     AVAATVGKLSWVVWTASIAMGFIQSFTYAEIAGLFPDKAGGTSIYGAIGWVRYGKLVA
                     PISVWCNWFAWSPVQATGSALAAGYILSGLFAPDAAINTWQITLLDLGSLKTGLSLRI
                     NAVFILGAVILLAVKFIQDGGILRSANTTKILGISAMIPLLLIGIVPLLTGDIVRANF
                     FPLAPLAHDALGRVADGTWNMAGITVFAAGMFLAAWSTYGFETAVCYTGEFKNPKTDT
                     FKAIFYSGLLCVVVYTLVPFAFQGYLGLGSLVHPAVIDAAGSITTPAQYSGMLAPEIN
                     SGMGVAAVMAKMVHAGRLVGAIVVIMLILALVLSLMTSMAGSSRTLYQASLDGWLPKY
                     LAKVNSHGAPTNAMLTNLGFNLLLLLLSDSVFIIGAANIGYLIFNFLNLNAGWIHRLD
                     RPRQERPWRAPTWILAAGALLSFVNLAFMGFGANVYGSGTLMTGLIFAALIVPVFVYR
                     HYIQDQGSFPRQMADDLDMVDGERIVKRAGIWPYVVLALGVAVVYVTHRLAVY"
     gene            complement(4554..5510)
                     /locus_tag="VGE92_13410"
     CDS             complement(4554..5510)
                     /locus_tag="VGE92_13410"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEY1314873.1"
                     /translation="MSGILVIAEQRRGELRPISLELVSAAQALRRSGEAVAVAIVNSV
                     PENFVKALSVAGVDEIVTVRVASAEFDPDTFESAIGALIAQLKPDVVLVAHSVDSFGY
                     AAALAARLNLGFATDVFNAERVDGELVATRGGYGQKVNVEVDFPGKSVVLLAIRGNVF
                     KAPDQPAAPRVTAFEAPAPASRATGREFIELSAGDDVDMSAAEFILTVGRGIGEEAKV
                     AQFRELADAVGATLGCSRPIADAGWLPKSRQVGQSGKTASACKLYIAMGVSGAIQHLA
                     GMKHVGTIVAVNTDAEASIFSVAKYGIVGDVFEIAEELRRLF"
     gene            complement(5507..6307)
                     /locus_tag="VGE92_13415"
     CDS             complement(5507..6307)
                     /locus_tag="VGE92_13415"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEY1314874.1"
                     /translation="MKILVAVKQVAALDEDFAIRDDGRDIDPDFFIRDLNEWDDFSLE
                     EAVKIKEAAAVPVEVVALSVGPDEVEESLRKCLAKGADRALRVWDPAVEDSDSIAIAR
                     VLAAAAKREAPDLMFAGVQSSDQSFASTGIAAAAFLGWPHAAVVSHLSYAPGSKLAVL
                     RRELEGGLLHEVEVQCPAVLTIQLGINTPRYASLRSIKQAAAKPIEVLSLADVGLAAG
                     DVGVAGSLSRVRRMYVPDKGRAQLIEGGAAQQAARLAEIIREFTGAGA"
     gene            complement(6304..8424)
                     /locus_tag="VGE92_13420"
     CDS             complement(6304..8424)
                     /locus_tag="VGE92_13420"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEY1314875.1"
                     /translation="MSRSDAVTRILFEAFGAAEVRIFYVIGYAAIAVFCYGVYVQVRK
                     YRRGAALALEGGLWPRCGAMLSSVLSHRTIERRDRPAGAAHRLIFYGFLLLFAGTATI
                     TLQYDILEPLFGIRFWHGEFYLVFSLILDVAGVALIAGLLYMMYRRGWLKPPKLDYAR
                     PDRAPGDPDFDRPQYRREDWAFLWLLLLIAGTGYVLEAARLVWLQDRTEVWDTRWWSP
                     LGALLAAGMRAAGLDAASAGLLRHGLWWFHGLIALVLIALIPYTKVKHIFTSAASLMV
                     RDPLAAQRLPRIPESQPQPGAAKITDLNWKQLLNLDACTKCGRCHEACPARAVGAPLS
                     PRDVILSLREFAHATLESGAIPAAAELDIHGKAPGQVAMETLWSCRTCMACVEICPVA
                     VEHVPIIVQMRRKLVEDGAMDPLLTKTLQTIHKTGNSFGESKRKRGGWSKALTFPVKD
                     ARKQPVDVLWFVGDYASFDPRNQKVSQLFATLLHEAGVDFGILFDGESNAGNDVRRVG
                     EEGLYEVLAQANVKALAGASFKSVVTTDPHSYNTIRNEYPDFGGRYEIEHYTTLTKRL
                     FAEGRLQVTKPLKYRVTFHDPCHLGRFNKGYDAPRELLALLGCELVEMARCKANSFCC
                     GAGGGRIWVPDPVGSEKPAQNRIKEAAAIPGLEVYVVSCPKDLTMFEDALKTTGNEGK
                     FVVKELIELIREAVPTRGTEEAPA"
     gene            complement(8424..10616)
                     /locus_tag="VGE92_13425"
     CDS             complement(8424..10616)
                     /locus_tag="VGE92_13425"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1314876.1"
                     /translation="MARDPKHDILFQPIKIGPKTLRNRFYQVPHCIGAGSDKPGFQAA
                     HRSLKAEGGWAGINTEYCSIHPESDDTHRLSARIWDAGDVRNLRAMTDHIHHFGSLAG
                     VELWYGASHAPCMESRATPRGPSQYASEFETLTYCKEMDLDDIATVQQYYVDAALRSR
                     DAGFDIVYVYGAHSYLPLQFLSPYYNKRKDKYGGSLENRARFWLETLEKVRRAVGHDC
                     AIATRFALDTLYGESGIEVENDGMKFIGLADSLVDLWDVDVGDIAEWGEDAGPSRFYQ
                     QGHQVPWTRFVKQVAKKPVLGVGRFTDPEKMTEIVTKGIADIIGAARPSISDPWLPKK
                     IEEGRYDDIRVCIGCNVCISRWEIGGPPMICTQNATAGEEYRRGWHPEKFPKRGSEDS
                     ILVVGAGPSGSECARVLLERGYTVHLYDTAAKIGGHLNSVVTLPGLGEWGYHRDYREL
                     QINKLVKKNKQSQVALGQKPMTAETALTYGAEKIVIATGSSWNTDGTNCLSHDPIPGA
                     DAAKPDQLTPEQVLEGKKALGKRVVILNADTYFMAPSLAEKLAVAGHDVTIVTGVHLA
                     NYMHFTLEYPNMMRRLHELHIEEMHNVFASRIETGRIEVYDIFGDGSKRSYRGRGQLP
                     REVNKSQRWLEFDSLVLVTGRHSHNALYTELKGRHGEWQKNGVKDVYLIGDAEAPRLI
                     ADATFSGQRLAREIEEAKAQLPLPYKREVAVWGTAHMPGGSFELEYKI"
     gene            10840..11496
                     /locus_tag="VGE92_13430"
     CDS             10840..11496
                     /locus_tag="VGE92_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003200568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEY1314877.1"
                     /translation="MRDPRPVKRPRAARAPKNPRAAPLSGGAPLNVGRQLGAVARRVR
                     ESQDLTLTDVASSAGISAGMLSRLETGHVTPSLETLVALAGALGVRPALLLQQLGGDE
                     EGAQRVPAGQGLEVVRRGTRRGHTYHLLAAQRGPRKSFEPFLVTLTDKSEVFPGFQHP
                     GTEFIYILGGSLTYRHGSHSYPLGAGDSLSFRGDVPHGPETLDKVPIRMLSIIIYDDE
                     "
     gene            complement(11481..12881)
                     /locus_tag="VGE92_13435"
     CDS             complement(11481..12881)
                     /locus_tag="VGE92_13435"
                     /inference="COORDINATES: protein motif:HMM:NF013788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HEY1314878.1"
                     /translation="MNETPTNLETPTGLDMPGNPSAPPGRASPPRGWFSALTRLWLLR
                     TLDAPPHGSALRAALLMAMLWLAAWVAIDRWQSQPDPKFFAGNIPLFAWYVLAVLGLS
                     AVLRWRVRPAPGFAPMLALAVAAVPLPLLLSSVLGIFLEPAWLMGAALLMALYTLLFL
                     RRGLQAVTGTEQRAAAFAGLIFFALFVWLSDALDVIPDVWAPAQSEAAQSAADVDGEA
                     ILFSQAKLIDESLAAVGRDVAPHPKAYFLGFAGVGDEKVFAQEIGLASRVLGERYGLA
                     GRSLSLINDERDLERAPLASVSGLRYALQGLGSLMDREQDVLFLAISSHGSQDPSIAV
                     ENSQLPLNDLSDEDLADALRESGIRWRVIIISACYAGGFIEALRDPQTIVITAAAADR
                     TSFGCSNDRDLTYFGEAFYRDALPEAESLREAFEAAKAAIDARERAEHITASKPEAYF
                     GAKIEAKLAAMRHSSS"
     gene            12964..13368
                     /locus_tag="VGE92_13440"
     CDS             12964..13368
                     /locus_tag="VGE92_13440"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY1314879.1"
                     /translation="MKVEGRCHCGAISYQAEIDPDGVRICHCTDCQTLTGSAYRVNVQ
                     TPAASFVLRSGQPKIYIKIAESGNQRAHAFCPDCGTPIYAAAAGPNPPNYALRVGTLK
                     QRHELKPKRQIWSRSQLPWVQDLHDVVRVDRQ"
     gene            13387..13914
                     /locus_tag="VGE92_13445"
     CDS             13387..13914
                     /locus_tag="VGE92_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003209603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEY1314880.1"
                     /translation="MALFQLGDVVPVIAASAYVAPGAVVIGKVHLADDSSVWFGATLR
                     GDNEMIHIGPGSNVQDGAVMHTDPGFPLHVAAHVSIGHQAMLHGCTVGEGSLIGIQAV
                     VLNAAVIGQGCLVAAGAIITERKVFPDGTLILGAPAKVVRELSAAEREDLVKIAANYA
                     ARGAYYRKQLRRIEE"
     gene            complement(13952..14323)
                     /locus_tag="VGE92_13450"
     CDS             complement(13952..14323)
                     /locus_tag="VGE92_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314881.1"
                     /translation="MSAAAAVAAPAPHAVSLTQDPVVMRLNKDEFRIAFGINGERCGN
                     AGCSGMIRYRVNWKTAEGIAHAETKQVSYQVSPLASRTIAVDRQYFDTAEGQHTTEVV
                     KVTIEAITCRDAIDLRTAKNL"
     gene            complement(14507..>14848)
                     /locus_tag="VGE92_13455"
     CDS             complement(14507..>14848)
                     /locus_tag="VGE92_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314882.1"
                     /translation="AASKGSADQVSAAAKGSADQVSAAAKGSADQVSAAAKGSANQVT
                     TAAKGSAVQVAAAAKGGADQVAAAAKGSADQVVAATKHGPEKTKAAAKVDKPDKSNDK
                     PLGEMDGMTLQ"
BASE COUNT         2761 a         4708 c         4754 g         2625 t
ORIGIN      
        1 cccgatacgt ccatgagacc ggccttggtg cggattgcca cgtgatcctg ggccacctct
       61 ttcgagtagg tccaagcggt ccccatgccg ttccaatcct caagcttcga tcccagtgcc
      121 cgatgccgat cggcgagagc cgaagtacgc cagctaagtg ccatagatgc ctccagagtt
      181 gttcattcag ggccggtgcc cggggtggtg ggcgagccgt catcgtaagg tgccagccac
      241 ttgaccgccg cgtcccgata acgcgtcaag cctttgtagt cgacgagcgc ggggtgcaga
      301 tcgcgcaaca cccggtgcac gcggcgcgcc cacttgggca ccgtggccag ctcgcgcaag
      361 ctgatcaagt agcaacgaat gccgaatacg atggcgttgc tgcgcggcag gcgccacagt
      421 ccctgcagct cgacgcgcag gtgcacgcgc tcgccggcat tgtccgccgt gaccgtggca
      481 cggtcggtgc cccagaacgg ataattttcc ggcgacgtgt cgagccgcgg gttgaccgtc
      541 atggtccaat tgagccgccg caccggctga cccaagcgaa gattgagcag gaatttcaac
      601 gcccgatcga atacctgcag ttcgtgcgcg agcggcaccg ggccatgcca ttccttgaat
      661 gtcatgccga tgtcgaagtc caatgaccag tcggcctgag tcgtgaccat gccggcctcg
      721 gtccacagat ctgcctcgcg ctgatccatg acgacgaagt cgccctgcgc ctggcgcgtg
      781 atgtagtcga gcggctcgcg cggcaggctc gaagtatcgc cgaatgtgaa ctgttgatcg
      841 atctgcaacg gccgattgat ccaatgccag cgattgccgc gtttcgagag cgaaaagtgc
      901 gcggggtagt ccctggcgag ggattccatg atcagttcca agcaatccca ttgcgaactc
      961 aacatatggg gcagcgcctg gtagcgcagc ggttcggcgg cgagcacgat ggcgcgttcg
     1021 cggcattccg ccacatagtg ctcatcgacg tcgaaggcaa attcagtgac cgatccaatc
     1081 gggccgggca gatgcggctc gatgttcacc gagtacatgt aggcgtcctc ggggaagggc
     1141 agcggaaagc gccgaatggc ctcggggctg ttgcggtagg tgaaatcgcc gcggaaggtt
     1201 tcgtcaggtt tgaacacagc gctcatcagt gctccttgca aggcgctaca ggtcgagtac
     1261 cagacgccga cagcgcgccc gcgacacaca gggcatgatg ctcttgccct tcgccttgtc
     1321 tgcatccgaa agaaaatgat cgttatggat gagctcgccg tcgagctcga gcacccgcgt
     1381 gtggcagaag ccgcacacgc cgccgcggca cagatacggc acttccactc ccgcggcctc
     1441 gatgctctcg agcaggcttt gatcgggcgg aacgtgcacc ttgaggtttg ctcgtgccag
     1501 gaatacctcg aaggcatcgc cgaccggggg tgcgctgaat tgttcccaat ggaggtggct
     1561 gtccggccaa ccgcaggcgt gcgccgtggc gatgacatgc ttgatcatgc cggccggacc
     1621 gcagacgtac acgtgcgtgc cgagcggttg cgaggcgagc atggcctcga acggaatcga
     1681 ctgtgcctcg ctgtcatagt acatgtgcaa tcgctcgccc tcgcgggcac gcagccgctc
     1741 caggaacgcc gcgtgctcgg gcgagcgcac cgagtagtgc agctcgtagg gaaccccgcc
     1801 gccatgcaag tcttcgagtt gcgcgaggaa cggcgtaatg ccgatgccgc cggcaatcaa
     1861 cacatgcttg cgcgcgatct tatcgagacc gaataaattg acaggatgcg cgatttccaa
     1921 acggctgccg accgcgaccg cgtcgtgcat gaagtgcgaa ccgccgcgcg actcttccat
     1981 gcggcgcacg ccgatttcat acgtttgcaa ctcatgcggc gcgctgagca gcgagtacgg
     2041 gttcttgtgc acccgatcgc cgccttgcat gacgacgatg atgtgcgagc cgccggagaa
     2101 cgccggcagg tcgccgccgc cgacgggcgc caacttgaaa tgcttgatga gcggcgtcac
     2161 ttgttcgatg ccgatgactt cgacggcgag ggtctgcgcg tcgctcacaa gaacacttcc
     2221 acgagcggcg gcaattcgcc cggtgcttcg gcatcgacca tgacgcccat atacgcggcc
     2281 agtcgccgcg aatagtgatc gcggaccaac agccagcgct cgcagccgac gcaacgcacg
     2341 atgttggtgc gcacgttttc ggtggtggcg cggcagtgaa tacagtagac gcggcgcgcc
     2401 aaggtgccgc attcttcggc gcggatctca tccttgttga gattgaactc gagcgcgatc
     2461 ttcatggcaa gaccgatgaa gctttccggt ccggccacat acagccgcgt gcccatgagc
     2521 gagcttgcca aactatgcct gaattcctcg agcagggcgg cggtggtggc gtaggagtct
     2581 gcgtggtcca cccccgcgtc ccggaattgt tcgactccgg ccggtgcacc tgcgcccgtg
     2641 ccacctgcgg catcgggcac gaacagcacc cgcgtattct caagagaggc agccgccgcg
     2701 ccgcgcatct ccgcgatcac ccgcagcagc ggggcggcgc ccacccccat gcccagcatc
     2761 aagtgatagc ggccgcgcgc gtccgcctgc agcggcgcat acacaggctt gcttttgatc
     2821 ccggtgacga gcattcaata caccgcgaga cgatgagtca cgtaaaccac ggcgactccc
     2881 aacgccagca cgacgtatgg ccagatgccg gcgcgcttga cgatgcgttc gccgtcgacc
     2941 atgtccaaat catccgccat ctggcgcggg aacgagccct ggtcctggat gtagtggcgg
     3001 tagacgaaca ccggtacgat cagcgccgcg aagatcaagc cggtcatgag cgtgcccgag
     3061 ccatacacat tggcgccgaa tcccatgaag gcgaggttga cgaaggacaa gagcgcgccc
     3121 gccgccagaa tccaggtcgg ggcgcgccac ggccgctcct gtcgcggacg gtccagccga
     3181 tggatccacc ccgcgttcag attcaggaaa ttgaaaatca gatagccgat gttggcggcg
     3241 ccgatgatga acaccgagtc ggacagaagc agcagcagca gattgaagcc gagattggtg
     3301 agcatggcgt tggtcggcgc accgtgggaa ttcactttgg cgagatactt gggcagccaa
     3361 ccgtcgagcg acgcttgata cagcgtgcgc gaagaacccg ccatcgaggt catgagcgag
     3421 agtaccagcg ccaggatcag catgatcacg acaatcgcgc ccaccagccg accggcatgc
     3481 accatcttgg ccatgaccgc cgcgacgccc atgccgctgt tgatctccgg cgccagcatg
     3541 ccgctgtatt gcgccggggt ggtgatgctg ccggcggcat cgatcaccgc cgggtgcacc
     3601 aaggacccaa gtccaagata accttggaag gcaaacggca ccagcgtgta cacgaccaca
     3661 cacagcaagc cggaatagaa gatggccttg aaggtgtcgg ttttgggatt cttgaattcg
     3721 ccggtatagc acaccgccgt ttcgaatcca taggtcgacc aggcggcgag aaacatgccc
     3781 gccgcaaaca ccgtgatgcc cgccatgttc caggtgccgt cggccacgcg gccgagggca
     3841 tcgtgtgcca acggcgcgag cggaaagaaa ttggctctga cgatgtcgcc cgtgaggagc
     3901 ggcacgatgc cgatcaagag cagtgggatc atcgccgaga tgccgaggat tttggtggta
     3961 ttcgccgatc gcaagatgcc gccgtcctgg atgaacttga ccgccagcaa gatgacggcg
     4021 ccgaggatga aaacggcatt gatgcgcagg ctgaggccgg tcttcaagga tccgagatcg
     4081 agcagcgtga tctgccaggt attgatcgcg gcgtcgggag cgaacagtcc gctcaagatg
     4141 tagcctgcgg ccagcgccga gcccgtcgcc tgcaccggcg accaggcaaa ccaattgcac
     4201 cacacggaga tgggcgcgac cagcttgccg tagcgcaccc agccgatcgc gccatagatc
     4261 gaggtgccgc cggctttgtc gggaaacaat ccggcaattt cggcgtaggt aaaggactga
     4321 atgaagccca tggcgatcga ggccgtccac acgacccacg acagcttgcc gacggtcgct
     4381 gcgacggcgc caatggagaa cagcaccaac ggcggcacgc cgctcgccac ccagaaggcg
     4441 ccggtccaat cgatgccgcg ctccaggccc gcgcccttca cgccgcttgc aactaccgcg
     4501 acgtcagcgg tcgagttcat acgaagcact ccgtgcagaa ttgaaaaaga tacctagaac
     4561 aagcggcgta actcttcggc aatctcgaag acgtcgccca caatgccata cttggcgacg
     4621 ctaaaaatcg acgcttcggc gtccgtgttg acggcgacga tggtgccgac gtgcttcatg
     4681 ccggccagat gctggatggc gccggaaaca cccatggcga tgtacagctt gcaggcgctc
     4741 gccgttttgc cggactgacc gacctggcgg ctcttcggca gccagccggc atccgcgatc
     4801 ggccgcgagc aacccagggt tgcaccgacg gcatccgcca actcgcgaaa ctgggccacc
     4861 ttggcctctt cgccgatgcc gcggccgacc gtcaagatga actcggcggc gctcatgtcg
     4921 acatcatcgc ccgcgctcaa ctcgatgaac tcgcgccccg tggcgcggct tgccggtgcc
     4981 ggcgcctcga acgccgtcac tcgcggcgca gcgggttgat cgggcgcctt gaagacattg
     5041 ccgcggatcg cgagcagcac cacgctcttg cccgggaagt ccacttcgac attgaccttc
     5101 tgaccgtagc cgccgcgcgt cgccacgagt tcgccgtcga cgcgctcggc attgaatacg
     5161 tcggtggcga agcccagatt caagcgtgcg gcgagcgccg ccgcatagcc gaaggagtcg
     5221 acgctgtgcg cgaccaacac cacgtcgggc ttgagctggg cgatcaatgc gccgatcgca
     5281 gactcgaatg tatccgggtc gaattcggcg cttgccaccc gcacggtcac gatctcgtcg
     5341 actccggcga cgctcaacgc cttgacgaag ttctcgggaa ccgagttcac gatggcaacc
     5401 gccaccgcct cgccgctgcg gcgcagagcc tgcgcggcgc tcaccaattc gagcgagatg
     5461 gggcgcagtt cgccgcgccg ctgttcggcg atgacgagta tgccgctcat gcccccgctc
     5521 cggtgaattc gcgaatgatt tcggccaggc gtgccgcctg ttgcgcggcg ccgccttcga
     5581 tcaattgtgc gcggcccttg tccggcacgt acatgcggcg cacgcgcgag agtgagcccg
     5641 caacgccgac gtcgccggcg gccaggccga cgtccgccag ggacagcacc tcgatgggtt
     5701 tcgccgccgc ctgtttgata ctgcgcaagg atgcgtagcg cggcgtgttg atgccgagct
     5761 ggatggtcaa caccgccggg cattggacct cgacctcatg caacaggccc ccctcgagct
     5821 cgcggcgcaa tacggccagt ttggacccgg gggcatagct caagtgactc accaccgccg
     5881 catgcggcca accgaggaac gccgcggcgg cgatgccggt ggacgcaaag gactgatccg
     5941 aggattgcac cccggcgaac atcaggtccg gtgcctcgcg cttggcggct gccgccagca
     6001 cgcgcgcaat cgcgatcgaa tcggagtcct cgacggccgg gtcccagaca cgcagcgcac
     6061 gatccgcccc cttcgccagg cacttgcgca gcgattcctc gacctcgtcc ggtcccaccg
     6121 agagcgcaac gacttccacc ggtacggcag ccgcttcttt aattttgacg gcttcttcca
     6181 acgaaaagtc gtcccactcg ttgaggtcgc gaatgaaaaa atccggatcg atatcgcgtc
     6241 cgtcgtcgcg gatcgcaaaa tcctcgtcga gcgccgcgac ctgtttgacg gccaccagga
     6301 ttttcatgcg ggagcctcct cggtaccgcg cgtgggaacg gcttcccgga tgagttcgat
     6361 gagttctttg accacgaatt tgccctcgtt gccggtggtt ttgagtgcgt cctcgaacat
     6421 ggtgagatcc ttcgggcagc tcaccacgta gacctcgagc ccgggaatgg cggcggcttc
     6481 cttgatgcga ttctgcgccg gtttctccga gcccacgggg tccggcaccc agatccggcc
     6541 gccgccggcg ccgcagcaga acgagttggc cttgcagcgt gccatttcga cgagttcaca
     6601 gccgagcagc gccaatagct cgcgcggcgc gtcgtaaccc ttgttgaacc gtccgagatg
     6661 acaggggtcg tggaaggtca cgcgatactt cagcggcttg gtgacctgca gccgcccttc
     6721 ggcaaacagc cgcttcgtga gcgtcgtgta gtgctcgatt tcataccggc cgccgaagtc
     6781 gggatactcg ttgcgaatcg tattgtaaga gtgcggatcg gtggtcacca cggacttgaa
     6841 cgatgcgccc gcgagcgcct tgacgttcgc ttgcgccagc acctcgtaca ggccttcttc
     6901 gccgacgcgg cgcacgtcat tgccggcgtt cgattcgccg tcgaataaaa tgccgaaatc
     6961 gacgccggct tcgtgcaaca gcgtggcgaa gagctgcgag accttttgat tgcgcggatc
     7021 gaaggaggcg tagtcgccga caaaccacag cacatccacc ggctgcttgc gcgcatcctt
     7081 gaccgggaag gtcagcgcct tgctccagcc gccgcgtttg cgcttggatt cgccgaatga
     7141 attgccggtt ttgtgaatgg tctgcagggt cttggtgagc aaggggtcca tcgcaccgtc
     7201 ctcgaccagc ttgcggcgca tttgcacgat gatcggtacg tgttcgaccg cgaccgggca
     7261 aatttcgacg cacgccatgc acgtgcggca cgaccagaga gtctccatgg ccacctgccc
     7321 cggcgctttg ccgtgaatgt cgagttcggc ggcggcgggg atcgcgcccg actcgagcgt
     7381 tgcatgcgcg aattcgcgca aactcaaaat cacatcgcgc ggcgagagcg gcgcgccgac
     7441 ggcgcgcgcg gggcaggctt catgacagcg gccacacttg gtgcaggcat cgagattgag
     7501 cagctgcttc caattcaaat cggtgatctt ggcggcgccc ggttgcggct gcgactcggg
     7561 aatccgcggc agccgttgcg cggccaaggg gtcgcgcacc atgagggaag ctgccgacgt
     7621 gaaaatatgt ttgaccttgg tgtacggaat caacgcgatg agcacgagcg cgatcaggcc
     7681 gtgaaaccac cacaacccgt ggcgcagcag gccggcggac gctgcgtcaa gccccgcggc
     7741 gcgcatgccc gctgcgagca gcgctcccag cggcgaccac cagcgggtgt cccaaacctc
     7801 ggtgcgatcc tgcagccaca cgagccgcgc ggcttcgagg acgtagccgg tgccggcgat
     7861 gagcaacagc agccagagaa aggcccaatc ctcacgccga tactgcggcc ggtcgaagtc
     7921 gggatcgccc ggcgcgcgat ccggccgcgc gtagtcgagt ttgggcggct tcagccaacc
     7981 gcgccggtac atcatgtaga ggaggccggc gatcaaggca acgccggcga cgtccagaat
     8041 cagcgagaac accaaataga actcgccatg ccagaagcgg atgccgaaca gcggctcgag
     8101 aatgtcgtat tgcagcgtga tggtcgcggt gcccgcgaac agcagcagaa atccgtagaa
     8161 tatcagccgg tgcgcggcgc ccgccggccg atcgcggcgc tcgatggtgc gatgggaaag
     8221 caccgacgac agcatcgcgc cgcagcgggg ccacaggcct ccctcgagcg ccaaggccgc
     8281 gccgcgccgg tatttgcgca cttgaacata gacgccgtaa caaaataccg cgatcgcggc
     8341 gtagccgatc acgtagaaga tccgcacttc ggccgcaccg aaggcctcga acagtatccg
     8401 agtgacggcg tccgagcggc tcatcagatc ttgtattcga gctcgaagct tccgcccggc
     8461 atgtgcgctg tgccccagac ggccacttcg cgcttatagg gcagcggtag ctgcgccttc
     8521 gcctcctcga tctcgcgcgc caaccgctgt ccgctgaaag tcgcatcggc gatcaagcgc
     8581 ggtgcttcgg catcgccgat caggtacacg tccttgacgc cgttcttctg ccattcgccg
     8641 tggcggccct tgagttcggt gtacagcgcg ttgtgtgaat gccgtccggt cacgagcacc
     8701 agcgagtcga actcgagcca gcgctggctc ttgttcactt cgcgcggcaa ttgcccccgc
     8761 ccgcgatagc tgcgcttgga gccgtcgcca aaaatgtcat acacctcgat acgccccgtt
     8821 tcgattcgcg aagcgaacac gttgtgcatc tcctcgatgt gcaactcgtg caggcggcgc
     8881 atcatgttcg gatactcgag cgtgaagtgc atgtagttgg ccaaatgcac cccggtgacg
     8941 atggtgacgt cgtgtcctgc gacagcgagc ttttccgcga gactcggcgc catgaaatag
     9001 gtgtccgcat tcaagatcac gacgcgcttg ccgagcgcct tcttgccctc gagcacctgt
     9061 tccggcgtga gttggtcggg cttggcggcg tccgcacccg ggatggggtc gtggctcagg
     9121 caattggtgc catccgtatt ccaggaagaa ccggtggcga tgacgatttt ctctgcgccg
     9181 taggtgaggg cggtttcggc cgtcatcggt ttttggccga gtgcaacctg actctgcttg
     9241 ttcttcttca ccaacttgtt gatttgcaac tcgcgatagt cgcggtgata gccccactcg
     9301 ccgagcccgg gcagcgtgac cacggagttc aagtgaccgc cgatcttggc tgcggtgtcg
     9361 tagagatgca cggtatagcc gcgctccaac aggacgcggg cgcattcgga accggaaggc
     9421 ccggcgccga ccaccaggat ggagtcctcg gagccgcgct tcggaaattt ctccggatgc
     9481 cagccgcgcc gatattcctc gccggcagtg gcgttttgcg tgcaaatcat cggcggcccg
     9541 ccgatttccc agcgtgagat gcacacgttg cagccgatgc agacgcggat gtcgtcgtag
     9601 cggccttcct cgattttttt cggcagccag ggatcggaga tcgagggccg cgccgcgccg
     9661 atgatgtcgg cgatgccctt ggtcacgatc tcggtcattt tctccgggtc ggtgaagcgg
     9721 ccgacgccga gcaccggctt cttcgccact tgcttgacga aacgggtcca cggcacctgg
     9781 tggccctgtt gataaaaccg cgacggaccg gcgtcctcgc cccactcggc aatgtcgccg
     9841 acatccacat cccacaaatc gaccagcgag tcggccaggc cgatgaattt catgccgtca
     9901 ttctcgacct cgatgccgga ttcaccatac aaggtgtcga gtgcgaagcg ggtggcgatg
     9961 gcgcaatcat ggccgaccgc gcggcgcacc ttctccaacg tctcgagcca gaagcgcgcg
    10021 cggttctcca aggaaccgcc gtatttgtcc ttgcgcttgt tgtagtaggg cgacaggaac
    10081 tgcagcggca gataggagtg cgcgccgtag acatagacga tgtcgaaacc ggcgtcccgt
    10141 gagcgcagtg cggcgtcgac gtagtactgc tgcacggtgg cgatgtcgtc caagtccatt
    10201 tctttgcaat aggtcagtgt ttcgaattcc gaggcgtact gactcgggcc gcgcggtgtg
    10261 gcgcgcgatt ccatgcacgg tgcatgcgag gcgccgtacc agagttcgac gccggccagg
    10321 gagccgaagt gatggatgtg atccgtcatg gcgcgaaggt tgcgcacgtc gccggcatcc
    10381 cagatgcgcg ccgacaggcg atgcgtgtcg tcggactccg gatgaatcga gcaatattcg
    10441 gtattgatgc ccgcccaacc gccttccgcc ttcagcgatc ggtgtgccgc ctgaaagccc
    10501 ggcttgtcgg aacccgcgcc gatgcagtgc ggcacctggt agaaccggtt gcgcagcgtc
    10561 ttgggtccga tcttgatcgg ctggaacaga atgtcgtgtt ttgggtcgcg cgccatactc
    10621 ttatcctctc gctgggtgga gcggtgttta tttgggaagg tattcgcctc tcaaggcgag
    10681 gaactcgtat catattctcg atcgatcagc gctggccagg tggccgcgag cgacccttca
    10741 ggagtccacg ggcatcggaa acaaatagca tgtcagttac tgtagtgcaa acttattgcg
    10801 tgcagtgtaa attcagttcc gagcataggg aagccgccgg tgcgtgaccc aagacccgtg
    10861 aaaaggccgc gtgcggcgcg cgcccccaaa aacccgcgcg ccgccccgct gtcaggcggc
    10921 gcgccgctca atgtcggccg gcagctgggg gccgtggcgc ggcgggtccg cgagagccag
    10981 gacttgacat tgaccgatgt ggcgagctcc gccggaatca gcgccgggat gctcagccga
    11041 ttggagaccg gccacgtcac cccgagtctc gagaccttgg tggcgcttgc gggcgcgctc
    11101 ggcgtgcggc cggcgttgct gttgcagcaa ttgggcggcg acgaggaggg ggcgcagcgc
    11161 gtgcccgcgg gtcaaggact cgaggtggtt cgacgcggca cgcggcgcgg gcacacctat
    11221 catctattgg cggcgcagcg cgggccgcgc aaatccttcg agcctttctt ggtgacgctc
    11281 accgacaaga gcgaagtgtt tcccggcttc caacaccccg gcacggaatt catttatatc
    11341 ctcggcggat cgctcaccta ccggcacggc agtcacagct atccgctggg ggccggtgat
    11401 tcgttgagtt tccgcggtga tgtgccccat ggtccggaga cgctcgacaa ggtgccgatt
    11461 cgcatgttgt cgatcatcat ctatgacgat gagtgacgca tcgcggccaa tttagcctcg
    11521 atcttggcgc cgaagtaggc ctcgggtttc gatgcagtga tgtgctcggc gcgctcccgc
    11581 gcgtcgatcg ccgccttcgc ggcttcgaag gcctcgcgca gcgactcagc ctccggcagc
    11641 gcgtcccggt agaacgcctc gccgaagtaa gtcaggtcgc gatcgttcga acagccgaac
    11701 gaggtgcgat ccgccgccgc ggccgtgatc acgatggttt gcgggtcgcg cagtgcctcg
    11761 atgaagccgc cggcgtagca ggcggagatg atgatgacgc gccaacgaat gccggattcg
    11821 cgcaacgcat ccgcgagatc ctcatcgctg agatcgttga gcggcagctg cgagttttca
    11881 accgcgatcg acggatcttg ggagccatgc gaggaaatcg ccaggaacag cacgtcctgc
    11941 tcgcgatcca tgagcgagcc caaaccttgc agcgcgtagc gcaggcccga tacgctcgcg
    12001 agcggcgcgc gctccaggtc gcgttcgtcg ttgatgaggg agagactgcg gccggcgagg
    12061 ccgtatcgtt ccccgagcac ccgcgaggcc aggccgatct cctgcgcgaa caccttctca
    12121 tcgccgactc cggcaaagcc cagaaagtag gccttcgggt gcggcgcgac gtcgcgtccg
    12181 acggccgcca aggattcgtc gatgagcttt gcctgcgaaa acagaatggc ctcgccgtcg
    12241 acgtcagcgg cggactgcgc tgcctctgac tgcgccggtg cccagacgtc gggaatgaca
    12301 tccagtgcgt cgctgagcca gacgaagagc gcgaaaaaga tcaggccggc gaaggcggcc
    12361 gcgcgctgct ccgtccccgt gacggcctgc agtccgcgcc gcaggaacag cagtgtgtac
    12421 agagccatga ggagcgccgc gcccatgagc caggcgggct cgaggaatat cccgagcacg
    12481 ctgctcagaa gcagcggcag cggcaccgcc gccacggcga gtgccagcat gggcgcaaat
    12541 cccggcgccg gccgcacgcg ccagcgcagc acggcggaca ggccgaggac tgcgagcacg
    12601 taccaggcga acagcggaat attgccggcg aagaattttg ggtccggctg cgactgccag
    12661 cggtcgatcg caacccaggc ggccagccaa agcatcgcca tcagcagtgc ggcgcgcagc
    12721 gcactgccgt gcggtggcgc atcgagggtg cgcaacagcc agaggcgtgt caacgcggaa
    12781 aaccaacctc gcggcggcga ggcgcggccc ggcggcgcgc ttgggtttcc cggcatgtcg
    12841 aggccggtcg gcgtttcaag attcgtcggc gtctcattca tgctgggcgc cgatcataac
    12901 agccctgtca gggccccccg gttctgcggt atcttcgatg gggggcatcc aaggagggtt
    12961 gaaatgaaag tcgaaggccg gtgtcactgc ggcgcgatca gctaccaggc cgagatcgat
    13021 cccgacggcg tgcgcatctg ccactgcacg gattgtcaaa ccttgaccgg ctctgcgtat
    13081 cgggtaaacg tgcagacgcc ggcggcgagt ttcgtgctgc gctccggaca accgaaaatc
    13141 tacatcaaga tcgccgaaag cggcaatcag cgcgcgcatg cgttttgccc ggactgcggg
    13201 acgccgatct atgcggcggc agccggcccc aatccgccca actatgcgct gcgcgtcggc
    13261 acactcaagc agcgccacga actcaaaccc aaacgccaga tatggtcgcg ctcgcagttg
    13321 ccgtgggtgc aagatcttca cgatgtggtg cgcgtcgatc ggcaatgaat tcgcggcgga
    13381 ggtctcatgg ctttgtttca attgggcgat gttgttccgg tgatcgccgc gagcgcgtac
    13441 gtcgcgccgg gcgctgtggt catcggcaag gtgcatctcg ccgacgatag cagtgtgtgg
    13501 ttcggcgcga ctttgcgcgg cgacaacgag atgatccaca tcggaccggg cagcaacgtg
    13561 caggacggcg cggtcatgca caccgacccc ggttttccgc tgcacgtcgc cgcccatgtc
    13621 tcgatcggcc atcaagccat gctgcacggc tgtacggtgg gcgagggttc gctcatcggg
    13681 atccaggcgg tggtgttgaa tgccgcggtc atcgggcaag gttgtctggt ggcggccggc
    13741 gccatcatca cggaacgcaa agtgttcccg gacggcacgc tgattttggg ggcgcccgcc
    13801 aaggtggtac gcgagctcag cgccgcggag cgcgaagatc tagtgaagat cgcggcgaac
    13861 tatgccgcgc gcggggctta ttaccgcaag caattgcggc gcatcgagga gtgaactgcg
    13921 ccggtatgcg agtgcacacc ggccagagcg gctacaggtt cttcgccgtg cgcaggtcaa
    13981 tggcgtcgcg gcacgtgatc gcttcgatgg tgaccttgac gacttcggtc gtgtgctgac
    14041 cttctgcggt gtcgaagtac tggcggtcga ccgctatggt gcggctcgcg agcggcgaca
    14101 cctggtagct gacctgcttc gtttccgcgt gggcgatacc ttcagcggtc ttccagttga
    14161 cccgataacg aatcatgccg ctgcagccgg cgttgccgca gcgttcgcca ttgataccga
    14221 aggcaatacg gaactcgtcc ttgttcaggc gcatgacgac gggatcttgc gtcagcgaga
    14281 ccgcatgggg cgcaggcgcg gcaactgccg cagcggcgct caatgtcaaa gcggcgacga
    14341 gggacagtga gaatggcttc atcggtaact ccgtttgtct gggcacgcac acacgatagc
    14401 ggcgcgccgg gtaggcgaca aacgatgaaa actcgctaaa tcctcgaatt tcctcatgtg
    14461 ttgaggcgga cggacagcgg cgcgcgacaa ggcgggccgc atcccctcat tggagcgtca
    14521 taccgtccat ttcgccgagc ggcttatcgt tcgatttatc cggcttatca acttttgcag
    14581 cggcttttgt tttctccggg ccgtgcttag tggcggcaac aacctggtcc gcgctcccct
    14641 tggcggcggc ggcaacctgg tccgcgcccc ccttggcggc ggcggcaacc tgaaccgcgc
    14701 tgcccttggc ggcggtggtg acctggttcg cactgccctt ggcagcggcg ctaacctggt
    14761 ccgcgctgcc tttggcagcg gcgctaacct ggtccgcgct gcccttggca gcggcgctaa
    14821 cctggtccgc gctgcccttg ctggcggc
//
LOCUS       DASWZT010000470         5463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_8541, whole genome shotgun sequence.
ACCESSION   DASWZT010000470 DASWZT010000000
VERSION     DASWZT010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8541"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VGE92_13460"
     CDS             <1..214
                     /locus_tag="VGE92_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314883.1"
                     /translation="EGGARRQLLRRDQQFEAASWTVEKNFIAVLHQRQRAAGIGLGSH
                     VQQADRGCDLDFLTGASGSTVERESH"
     gene            214..1179
                     /locus_tag="VGE92_13465"
     CDS             214..1179
                     /locus_tag="VGE92_13465"
                     /inference="COORDINATES: protein motif:HMM:NF016894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HEY1314884.1"
                     /translation="MGGASFVAGDWGTSNLRLFLCDADGAALDQLTGPGVAEVAGSSG
                     AAGFADTFDSLTAPWQAAHAVLPAVLCGMVGSSIGWIQAPYVPCPAMPEQIIDACTAL
                     PGARIHIVPGLSCRNRFNAPDFMRGEETQILGALTRVPELSRGTRLLCLPGTHTKWVV
                     LVDGLLRDFFTAPSGELFALLCEHSVLVRKPKGDAPADLAAFQAGLARFNEFPNAQLL
                     HRLFECRSRQLSGELAAQSADAYLSGLLIASDVHGALGALSGAVAERSVCLIGTDRLL
                     QLYALALQSHGFAALPVEGAAAALAGLSRVQQRLTLAQHQVTHGA"
     gene            1169..1801
                     /locus_tag="VGE92_13470"
     CDS             1169..1801
                     /locus_tag="VGE92_13470"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF006600.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HEY1314885.1"
                     /translation="MALDIAADATVLVAILRGVSPLRVIEIGDVLYEAGFRTLEVPLN
                     SPDPFASIAKLAAHRSDWTIGAGTVLEPEDVRRTRDAGGRLVLAPNCDAAVIRRALEL
                     DLQVIPGIATATEAFVAIAAGAQQLKLFPASTYGPRHLGALSAVLPGEARVFPVGGIG
                     AQDIAAWLAVGAAGFGFGSELFRTDYSVADIARRARELVQALRDARAKTP"
     gene            1824..3386
                     /locus_tag="VGE92_13475"
     CDS             1824..3386
                     /locus_tag="VGE92_13475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/sugar symporter"
                     /protein_id="HEY1314886.1"
                     /translation="MKLPVLDVAIIGVYAVSIFTLAQWVSREKGAHEKTAQDYFLASR
                     ALPWWAIGTSLIAANISAEQIIGMSGSGYVIGLGIASYEWMAALTLLIVGKFFLPIFL
                     KNDIYTMPEFLERRYSPAVRTVMAIFWLGVYVFVNLTSILWLGATAVHTVAGISVQAA
                     LIALGLFAGAYALYGGLKAVALTDIVQVSLLVLGGLVISYITLNKISGGAGVLAGFHD
                     LTVRFPEKFVMILPRSSPSYKDLPGVSVLIGGLWVMNVSYWGFNQYIIQRALAAKSIR
                     EAQKGIVLAAFLKILMPVIIVLPGIAAAALAPNLGRADEAYPHLMGMLPSGLLGLVFA
                     ALIAAIVASMGSKINSIATIFTMDVYKPWRPTTEQRQLVLIGRITAAVALVVAILMAK
                     PLVGNFDQAFQYIQEFTGFFTPGICVIFLLGMFWERCTVSGALVAAIASAVLSLALKL
                     TWPSLPFMDRVGLVFLACSVIAVALSLLQERRDPALRVELKGIDYSTSAGFNVGALAV
                     IAVLIVVYTVWW"
     gene            3398..4276
                     /locus_tag="VGE92_13480"
     CDS             3398..4276
                     /locus_tag="VGE92_13480"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY1314887.1"
                     /translation="MAALSPIDIVMTENILGEGILWDSRRETLWWTDIQGRRLHRYDW
                     TRRTLELLATPERVGSFGLVAGSQRLIAAFASGIALYDVRQQSVDWLTGIEALGPGVR
                     FNDGRVDRRGRFWAGTMVEGGQRSADACLYCITAGDARCHVRDVRVANGLCTSADGTR
                     LYFADSPTRTILEYRLNEPDGMLSAPRVFARTPQGAFPDGATVDVDGCVWSAHWGGSC
                     VVRYTPEGRIDRTLAVPTRQPSCVCFAGPDLDVLCVTSAREGLDAATLGAEPHAGDVF
                     FYRPGVQGLPESEYCA"
     gene            4273..5013
                     /locus_tag="VGE92_13485"
     CDS             4273..5013
                     /locus_tag="VGE92_13485"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEY1314888.1"
                     /translation="MTASTHGRNLTHGVANSLGAAIVTGVYCADNPIPNEADLCVQYR
                     TSHSVVREAVKMLTAKGLLGSRPRRGTWVQPEADWNLLDPDVLGWLLERKYSPALLLE
                     FTELRLAVEPVAAALAARAAGPKEKAAIRAAIERMHAAERGEDHPLDSDIAFHIAVLR
                     ASRNRFYAQFTGFVATALSFSIRTTNRYKGVELASVADHQRVADAILGGRPAVAEKAM
                     RTLIEEAIELINRRDAVQARRLSANERR"
     gene            5014..>5463
                     /locus_tag="VGE92_13490"
     CDS             5014..>5463
                     /locus_tag="VGE92_13490"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY1314889.1"
                     /translation="MAKIKIAIVGLGKIARDQHVPTLAASDDFELVAVASPYDSLEGI
                     PSFKDVESMLCAMPQVEAVALCTTPQVRYPLAHFALLERRHVLLEKPPGVTLGEVRAL
                     EDLAKRQGVTLLTSWHSRFARGVEPARAWLAARKISRVAITWKEDVRV"
BASE COUNT          881 a         1740 c         1768 g         1074 t
ORIGIN      
        1 agagggcggc gcgcgccgcc agctcctgcg ccgtgaccaa cagttcgagg cggcgagctg
       61 gaccgtcgag aagaatttca tcgccgttct gcaccagcgc cagcgggccg ccggcatcgg
      121 cctcgggagc cacgtgcagc aggccgatcg cggttgcgat ctcgactttc tgacaggtgc
      181 gagcggcagc acggtggagc gggaatcgca ctgatgggcg gcgcgagttt cgtcgccggc
      241 gattggggca cgagcaacct gcgcctgttc ctgtgcgatg ccgacggggc ggcgctcgat
      301 cagctgactg gtccgggcgt ggccgaagtc gctggcagca gcggcgccgc gggcttcgca
      361 gacaccttcg attcgctcac ggcaccgtgg caggcagcgc atgcagtgct gccggccgtg
      421 ctctgcggca tggtcggctc gagcatcggc tggatccagg cgccgtacgt gccctgccct
      481 gccatgccgg agcaaatcat cgatgcctgc actgcattgc ccggcgccag gattcacatc
      541 gtacccgggc tctcctgccg caatcgattc aacgcaccgg acttcatgcg cggcgaagag
      601 acgcagattc tcggggcgct tacccgagta cctgaactgt cgcgcggaac ccgcttgttg
      661 tgcctgcccg gcacccacac caagtgggtg gtgctcgtgg acgggttgct gcgcgatttc
      721 ttcacggcgc cgagcggcga gctgtttgcc ttgctttgcg agcacagcgt tctggtgcgc
      781 aagcccaagg gcgacgctcc ggccgacctt gccgccttcc aagcgggcct cgcgcgcttc
      841 aatgagtttc cgaacgcgca gctgctgcac cggttgttcg aatgccgcag ccgccagctg
      901 agcggcgaac ttgccgcgca atcggcagac gcgtatttgt cgggtctcct cattgcgagc
      961 gatgttcatg gcgcattggg cgcgctatcc ggtgcggttg ccgagcgctc ggtgtgcttg
     1021 atcggcaccg accggctcct gcagctgtac gcgctcgcct tgcagtccca tggctttgca
     1081 gcattgcccg tcgagggcgc ggcggcagcg cttgccgggc tgtcacgggt gcagcaacga
     1141 ctaacgctag cgcaacacca ggtgacccat ggcgcttgac atagcggccg acgcaacagt
     1201 gctggtcgcg attctgcgcg gcgtttcccc tttacgggta atcgagattg gagacgtgct
     1261 gtacgaggcg ggctttcgca cgctcgaagt accgttgaac tcgcccgatc cgttcgcgag
     1321 catcgccaag ctcgcggctc accgctccga ttggaccatc ggcgcgggca cggtgctcga
     1381 gccggaggat gtgcgccgga ctcgcgacgc cggtggccgc ctggtgcttg cgccgaactg
     1441 cgacgccgcg gtgatccgtc gcgctctcga gctcgacttg caggtaattc cgggtatcgc
     1501 gaccgccacg gaggcttttg tcgccatcgc ggccggcgcg cagcaactca aattgtttcc
     1561 cgcctcgact tacgggccgc gacatttggg cgcgctctcg gccgtcctgc cgggcgaggc
     1621 ccgcgtgttt ccagtgggcg gcatcggcgc gcaagatatc gctgcgtggc tcgccgtcgg
     1681 cgcggcgggg ttcggcttcg gttccgagct tttccgcact gattacagcg tcgcggacat
     1741 cgcgcgccgc gcccgcgaac tggttcaagc cttgcgggac gcccgcgcca agactcccta
     1801 acaaaatcga tcaggatgca tttgtgaaac ttcccgtcct cgatgtcgcc atcatcggcg
     1861 tgtatgccgt gtcgatattc actctggcgc aatgggtgtc gcgcgaaaaa ggcgcccacg
     1921 agaagaccgc tcaggattat tttctcgcca gccgcgcgct tccttggtgg gcaatcggca
     1981 cctcgctgat cgccgccaac atttctgcgg aacagatcat aggcatgtcc ggctccggct
     2041 atgtgatcgg tctcggaatt gcctcctacg agtggatggc ggcgctcacg ctgctcatcg
     2101 tcggcaaatt ctttctgccg atatttctca agaatgatat ttatacgatg cccgagttcc
     2161 tcgagcgacg ctacagcccc gccgtgcgca cggtgatggc gattttttgg ttgggcgtgt
     2221 acgtgttcgt caatctcacc tccatcctat ggctcggcgc aacggcggtt cacaccgtcg
     2281 ccgggatcag tgtacaggcc gcgttgatcg cgctcggttt attcgccggc gcgtacgcac
     2341 tgtacggcgg cctcaaggcc gtggcgctca ccgacatcgt gcaggtgtcg ttgctggtac
     2401 tcggcggact ggtcatcagc tacatcaccc tcaataaaat atccggcggc gcgggggtgc
     2461 tcgccggctt tcacgacttg acagtgcgat ttcccgagaa attcgtgatg atcctgccgc
     2521 gcagcagccc tagctacaag gatctgcccg gcgtatcggt gctgatcggc gggctgtggg
     2581 tgatgaacgt gtcttactgg ggcttcaacc aatacatcat tcagcgcgcg cttgcggcga
     2641 agagtatccg ggaagcgcag aagggcatcg tgcttgcggc tttcttgaag atactgatgc
     2701 cggtgatcat cgttctgccc ggcatcgcgg ccgcggcgct ggcgccgaat ctcggccgcg
     2761 ccgacgaggc ctatccacac ttgatgggca tgctgccgag cggcctgttg gggctggtct
     2821 ttgcggcgct gatcgccgcc atcgtcgcct ccatgggttc gaagatcaat tccattgcca
     2881 cgatcttcac catggacgtc tacaagccgt ggcgtccaac aaccgagcag cggcagttgg
     2941 tgctcatcgg gcgcatcacc gcggcggttg cgctggtcgt cgccatcctc atggccaaac
     3001 ccttggtcgg caactttgat caggcattcc agtatatcca ggaatttacg ggctttttta
     3061 cccccggcat ctgcgtgatc ttcctgctgg gaatgttctg ggagcgctgc accgtgagcg
     3121 gcgcgctggt cgcggccatt gcctcggcgg tgttgtcgct cgcactgaaa ttgacctggc
     3181 cgtccttgcc gttcatggat cgcgtgggcc tggtattcct cgcttgcagc gtcattgcgg
     3241 tcgccctatc gttgctgcaa gaacggcgcg atcccgcatt gcgcgttgaa ttgaagggaa
     3301 tcgactattc gacgagcgcc ggcttcaacg tcggggcact cgccgtcatt gccgtcttga
     3361 tcgtcgtcta cacggtttgg tggtagggaa gagcaccgtg gccgccctct caccgatcga
     3421 catcgtcatg accgagaata tcctcggcga aggtatctta tgggactcgc gccgcgagac
     3481 cctgtggtgg accgacatcc agggacgccg gttgcatcgc tacgactgga cccgacgcac
     3541 gctcgagctg ctcgctactc cggagcgcgt tggttcgttc ggattggtcg ccggcagcca
     3601 gcggctcatc gcggcattcg cctccggcat cgcgctctac gacgtccggc aacaatcggt
     3661 cgattggctc acgggaatcg aggcgctcgg acccggtgtt cgtttcaacg atggcagggt
     3721 cgatcgtcgc ggccgatttt gggccggcac catggtggaa ggcggccagc gcagcgcgga
     3781 cgcctgcctg tattgcatca ccgccggtga cgcgcgttgc cacgtgcgcg acgtcagggt
     3841 ggccaatggg ctgtgcacga gtgccgacgg cactcggctg tatttcgccg actcgccgac
     3901 gcgaaccatt ctcgagtacc gcttgaacga acccgatggg atgctgagtg cgccgcgagt
     3961 cttcgcgcgc actccgcagg gggctttccc ggacggtgcc acggttgacg tggacggttg
     4021 cgtgtggagt gcgcactggg gcggcagctg cgtcgtgcgc tatacccccg agggccgcat
     4081 cgaccgcacg ctggcggtgc cgacccgcca acccagctgt gtttgctttg ccggtccgga
     4141 cctcgacgtg ctgtgcgtca ccagcgcgcg cgaaggcctc gacgctgcaa cgctcggtgc
     4201 cgaaccgcat gccggcgacg tgttcttcta ccgccccggc gtgcaaggat tgcctgagtc
     4261 ggagtattgc gcatgacagc ctcgacacac ggccgcaatt tgacccatgg ggtcgccaac
     4321 agtctcggcg cggccatcgt gacgggtgtc tattgcgccg acaatcccat cccgaatgag
     4381 gccgatctgt gcgtgcagta ccgcaccagt cattcggtgg tccgcgaagc cgtcaagatg
     4441 ctgaccgcga aagggctgct gggatctcgg ccgcgccgcg ggacttgggt gcagccggaa
     4501 gcggattgga atctactcga tcctgacgtc ctcggatggc tgctcgaacg aaagtactcg
     4561 cctgcactgc tgctcgagtt cacggagtta cgactcgcgg tcgagccggt agcggctgcg
     4621 ctggcggcgc gcgcggccgg ccccaaggag aaagccgcca tccgcgccgc catcgaacgg
     4681 atgcacgccg ccgagcgtgg cgaagaccat ccgctcgact ccgatatcgc cttccacatc
     4741 gcggtgctgc gggccagccg caaccgcttc tatgcacagt tcacgggatt tgtggcgacg
     4801 gcgctcagtt tcagcatccg caccacgaat cgctacaaag gagtcgagct cgcgagcgtc
     4861 gccgatcatc agcgcgtagc ggatgcgatt ttgggcggcc ggccggccgt cgccgaaaag
     4921 gcgatgcgca cgctgatcga agaggccatt gagctcatca accgacgcga cgccgtacaa
     4981 gcgcgccgct tgtccgccaa cgagcggcgt tagatggcca agatcaaaat cgccatcgtc
     5041 ggactcggca agattgcgcg cgatcagcac gtgccaacgc ttgcggcgag cgatgacttc
     5101 gagttggtcg cggtcgcgag tccttacgac agtctcgaag gaattccgag cttcaaagat
     5161 gtcgagtcga tgttgtgcgc aatgccgcag gtcgaagccg tagcgctgtg cacgacgccg
     5221 caggtgcgct acccgctggc gcatttcgcc ttgcttgagc gtcggcacgt gctgctggag
     5281 aaaccccccg gcgtcacgct cggcgaagtc cgggcactcg aggatttggc gaaacggcaa
     5341 ggcgtgacgc tgctgacatc gtggcactcg cgtttcgcgc gcggcgtcga accggcgcgc
     5401 gcgtggctcg ccgcgcgcaa gatttcgcgc gtcgcgatca cttggaaaga ggacgtgcgc
     5461 gtg
//
LOCUS       DASWZT010000471         2955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_47380, whole genome shotgun sequence.
ACCESSION   DASWZT010000471 DASWZT010000000
VERSION     DASWZT010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47380"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1970)
                     /locus_tag="VGE92_13495"
     CDS             complement(18..1970)
                     /locus_tag="VGE92_13495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY1314890.1"
                     /translation="MSTNLTRRCVGLVVIAGFLLKTLASAAQTLPSETPAQFVPRVDS
                     FDYVKREVMIPMRDGVKLETVILIPRGARRAPILLTRTPYGASERLIKNSSAHLAALI
                     DSSDVADDAVVNGGYIRVMQDVRGKHGSEGDYVMTRPLRGPLNPTAVDHSTDTYDTID
                     WLVKNIPETNGKVGILGISYDGFTSLMALVNPHPALRASVPINAMVDGWRGDDWFHNG
                     AFRQDSLTYFHGQESTRASELSWWTDHYDDYDAWLSVGSASDMARLHGLEQVGFTDKV
                     MNHPAYDAFWQQQALDKILAKQGVTVPTMLVHSLWDQEDIYGNIALYKALKAQQPQNP
                     DLYLVIGPWFHHQQRLDGSALGAIRFGSDTAQYFRLHVLRPFLDHYLKDEPTPVQITP
                     VTAFETGTNRWLALTTWPSGCSGGCAVEHQPLYLQAGGTLKFGAAGAAAADFESYVSD
                     PAKPVPYLPRPIHIAGDEGETSWQTWLVSDQRHVASRTDVLSFATPPLKQPLKISGEP
                     IANLIASTSGTDGDFVVKLIDVYPEEVGREPKMGGYQLMVSADILRGRYRESLSNPKP
                     IPADQKQVYRFALPTANHVFLPGHRIMVQVQSSWFPLYDRNPQSYVDNIFYAKPADYK
                     KATIKIFDGAANASFVELPVVSGATP"
     gene            2222..>2955
                     /locus_tag="VGE92_13500"
     CDS             2222..>2955
                     /locus_tag="VGE92_13500"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY1314891.1"
                     /translation="MNNLSFSVADGVATLSLQRSAQCNAIDITIIRAMHLALDQIQKG
                     EGGARAVVITGSPEVFCSGLDLHSIDLKTHEARQRAHFEMRRFMDPLILRLSGFRFPI
                     VAAVNGAAVGAGMSLALASDIIIAAEDAYFLPSFARLGLVPDAGITFHLARRIGVGRS
                     LSSLMLAERIDAKTALEWGLAYAVVPAADVVARAQAVARKLAAGPCAVLGQIRTLHAS
                     TFDNSLQEQLRAERSAQEMTLETKDC"
BASE COUNT          515 a          897 c          962 g          581 t
ORIGIN      
        1 gtccgactcg cgccgcgcta cggcgtggcg ccgctcacga ccggcaattc gacaaaactc
       61 gcgttcgcag caccgtcgaa tatcttgatg gtcgccttct tgtagtcggc cggcttcgcg
      121 tagaaaatat tgtcgacata gctctgcggg ttgcggtcgt agagcgggaa ccaactcgat
      181 tgcacctgta ccatgatgcg atgtccgggt aggaatacat ggttcgcggt cggcagcgcg
      241 aagcgataca cctgtttttg atccgccggg atgggcttcg ggttgctcaa cgattcgcga
      301 tagcgcccgc gcaggatgtc ggctgacacc atcagttggt atccgcccat tttcggttcg
      361 cgccccacct cctccggata cacatcgatc aatttcacca cgaagtcgcc gtcggttccg
      421 gaggtcgagg cgatcagatt ggcgatcggt tcaccgctga tcttgagcgg ttgtttgagc
      481 ggcggcgtgg cgaaactcaa gacatcggtt cgcgacgcga catgccgttg atcgctcacc
      541 agccaggttt gccagctggt ctcgccctca tccccggcaa tgtgaatggg acggggcaga
      601 tacggcaccg gcttggcagg atccgagaca taggattcga agtcggcggc cgcagcgccc
      661 gcggcgccga acttgagagt cccgcccgct tgcaggtaca gaggttgatg ctcgaccgcg
      721 caaccgccgc tgcaaccgct cggccaggtg gtgagcgcca accagcgatt ggtgccggtc
      781 tcgaaggcgg ttaccggcgt tatctgcacg ggcgtgggtt catccttgag ataatggtcc
      841 aagaacgggc gcagcacgtg cagccggaaa tactgcgccg tgtcgctgcc gaagcggatc
      901 gcgccgagcg cgctgccatc gagccgctgc tgatggtgga accacggccc gatgaccaga
      961 taaagatccg gattttgcgg ctgctgcgcc ttgagcgcct tgtatagcgc aatattgccg
     1021 tagatgtcct cctgatccca caggctgtgc accagcatcg tgggtaccgt gacgccttgt
     1081 ttggcgagaa tcttgtccag ggcttgctgc tgccagaagg cgtcgtacgc agggtggttc
     1141 atgaccttgt cggtgaagcc cacttgttcg aggccatgca ggcgcgccat gtcgctcgcc
     1201 gagccgacgc tcaaccaggc gtcgtagtcg tcgtagtggt cggtccacca cgacagctcg
     1261 ctggcgcgcg tggattcctg accatggaaa taggtgaggc tgtcctgtcg aaacgcgccg
     1321 ttgtggaacc agtcatcgcc gcgccagcca tcgaccatgg cgttgatcgg taccgaggcg
     1381 cgcagcgccg gatggggatt caccagcgcc atcagggagg tgaacccgtc gtaggagatt
     1441 ccaagaatgc cgaccttgcc gttggtttcg gggatgttct tgaccaacca atcgatggtg
     1501 tcgtaggtgt cggtcgagtg gtccacggcg gtcgggttca gcggcccgcg cagcggacgg
     1561 gtcatgacgt aatcgccttc cgaaccatgc ttgccgcgca catcctgcat cacgcggatg
     1621 tagccgccgt tgaccaccgc gtcatcggca acatcgctgc tgtcgatcag ggccgccaag
     1681 tgcgcgctgc tgttcttgat gaggcgctcg ctggcaccgt agggagtgcg cgtcagcaga
     1741 atcggcgcgc gccgtgcgcc gcgcgggatg agaatcacgg tttcgagctt gacgccgtcg
     1801 cgcatcggga tcatgacctc ccgtttcacg tagtcgaagc tgtccacccg cggcacgaac
     1861 tgcgccgggg tttcgctcgg cagcgtctgc gcggcgctgg ccagcgtctt gagcagaaaa
     1921 ccggcgatta ccaccaggcc gacgcagcgg cgcgtaaggt tcgtcgacat cgttgcactc
     1981 cggagaattg ctggcggcgg agtgtgcggc atcaggcggt gctctgcaac ggcgacccgg
     2041 aggtcgaccg gggcccaagc gggggccgcg agtcgagaac taatcattcg tgctcgacat
     2101 attgctcggc caaccgttaa ccgctctgct tgcggcagcg ggcgtctcgg gggcatgatc
     2161 caggcacggg gctcatcgac cggggtcaat tgctcgagtt ggcggatgga ggggctggca
     2221 aatgaataac ttatcattct cggttgcgga cggcgttgca actctgtcgc tgcagcgctc
     2281 ggcgcagtgc aacgccatcg acatcacgat cattcgcgcg atgcacctgg cgctcgatca
     2341 aatacaaaaa ggcgagggcg gcgcccgcgc ggtggtcatc accggcagcc ccgaggtatt
     2401 ttgcagcggc ttggacctgc attcgatcga tctgaaaacg cacgaggcgc ggcagcgggc
     2461 gcatttcgaa atgcgccgct tcatggaccc attgatcctg cgtttgagcg gctttcgctt
     2521 tcccatcgtg gctgcggtga acggcgccgc ggtgggtgcc ggcatgagcc tcgcgctcgc
     2581 cagcgacatc atcatcgccg cggaggatgc ctatttcctg ccctcgttcg cgcggcttgg
     2641 gctcgtgccc gatgccggga tcacttttca tctcgcgcgg cgcatcggtg tcggccgctc
     2701 gctgtcctcg ttgatgctgg ccgaacgcat cgatgcgaag acggcgctcg aatggggcct
     2761 tgcttatgcg gttgtgccgg ccgcggacgt ggtcgcgcgc gcgcaggcgg tggcacgcaa
     2821 gctcgccgcg ggtccatgcg cggtactcgg ccagattcgt accctgcacg cctcgacctt
     2881 cgataattcg ctgcaagaac aactgcgcgc cgaacgcagt gcgcaggaaa tgaccttgga
     2941 gacaaaggac tgcat
//
LOCUS       DASWZT010000472         6457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_76236, whole genome shotgun sequence.
ACCESSION   DASWZT010000472 DASWZT010000000
VERSION     DASWZT010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76236"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VGE92_13505"
     CDS             <1..193
                     /locus_tag="VGE92_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314892.1"
                     /translation="GYVAAALLAAIEWGLKPVMARAQERGTAFGLGFGAWHGVSALLY
                     LLACLAVAVLLWKDSRVPA"
     gene            complement(190..3069)
                     /locus_tag="VGE92_13510"
     CDS             complement(190..3069)
                     /locus_tag="VGE92_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314893.1"
                     /translation="MFRINLQARVILLAISALLASCGSSSGSQSPAKPLADTPPTAPG
                     GLTATVVSSIQINLAWTASTDDAGVAGYQLERCQGAGCTNFAQIAAPISTTWSDTGLT
                     GSLSYSYRVRAADSAGNFSAYSGIVSATTPLPPDTAPPTAPSALASSAVSSTEINLSW
                     TASTDNVGVTGYLVERCQGAGCTNFAQIAATAATTLTDSSLTGAMSYSYRVRATDAAG
                     NLSAYSGIASAMTPLPPDTTPPSAPANLNATLVSSTQVTLVWTAATDNVGVTGYLVER
                     CQGAGCSNFAQVATANSPTWSDAAVTAATAYSYRVRATDAAGNLSGYSNIVSTTTANA
                     VAVTVTPVRGGLTISQTLTLTAAVANDVGAAGVTWSASAGTFSAQTSSTASYVAPNTA
                     GQVTITATSVADSTKSATATLGVTDLNLVGTFHNDNSRDGANTQEYALTKANVNTASF
                     GKLFSCQVDGAIYAQPLWISNLTINGAQHNVIIVATQHDSVFAFDADASPCVTLWQAS
                     LLDASHGATPNETSVPSGGPTSLVGSGYGDVKPEIGITGTPVIDPTTNTLYVVSKSAV
                     ANALPIYQRLHALRLTDGTETAGGPTVIDGSIYVTGTAVDANSNSQVVFNPQTEFQRP
                     GLTLVNGTVYIAWASHEDTDPYHGWVMGFNASTLALVPNAVFNTSPNQVSGYTYARGG
                     IWMSGGAPTVDAGGNLYFSTGNGVFDADTGGANFGDSTLKLSTASGLAVADWFTPADE
                     AALDSADRDHGSGGAALLMNTPSAGYVVVAGKEGIVYVLSQSSLGHYGANATPLNSNV
                     HQEFSLGHGLFSTAGFWNNTLYVAGSGSGLQTYTFDPVAGQFAVGASSISHHVYGWPG
                     ASPTISAAGTNNGVAWAIDSHPYCTQQSSNCGPAVLYAMDAGNLTSELWNSSMVPADQ
                     AGNAVKFTVPTVANGKVYVGTRGNDIGTGTASVLGELEVYGLKAN"
     gene            complement(3223..3519)
                     /gene="yhbY"
                     /locus_tag="VGE92_13515"
     CDS             complement(3223..3519)
                     /gene="yhbY"
                     /locus_tag="VGE92_13515"
                     /inference="COORDINATES: protein motif:HMM:NF014087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEY1314894.1"
                     /translation="MALSEKQKKHLRRLAHPMSPIVMLGNAGLTDGVVSELDRALTDH
                     ELVKVSARIGARDARNATLAMLAGRTHAELVQRIGHVGVFYRRRAELPKILIPD"
     gene            3544..4170
                     /gene="rlmE"
                     /locus_tag="VGE92_13520"
     CDS             3544..4170
                     /gene="rlmE"
                     /locus_tag="VGE92_13520"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007953751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HEY1314895.1"
                     /translation="MPKRSKSSSRWLAEHASDEFVKRAQKEGWRSRAVFKLAQIQESE
                     RLLRPGMRCVDLGAAPGGWSQYAARIIGGSSRIVATDILAMDAIPGVDFVQGDFRETA
                     VLEQVLDCVGADKVDLVLSDMAPNMAGIDAVDQPRSMYLAELALEFADRVLAPGGDLL
                     LKLFQGAGFDQIIRDTRKRYARVVMKKPKASRTRSPEIYLLARQFRMV"
     gene            4236..6149
                     /gene="ftsH"
                     /locus_tag="VGE92_13525"
     CDS             4236..6149
                     /gene="ftsH"
                     /locus_tag="VGE92_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013974289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY1314896.1"
                     /translation="MNDLTKQILLWVALAVILLAIFSQFGMRTGQPSTIDYSQFLSEV
                     KSNNVDSVTLKGEIIEGKRRSSGESFVTYNPETSNTALIGLMQSQGVKFRGEPPKQQS
                     VFLQLLISAFPILILIGFWAYFMRQMQGASGGRGAMSFGKSRARLLGEDQVNITFADV
                     AGIEEAKQEVVEIVDFLKDPGKFQKLGGKIPKGVLMVGSPGTGKTLLARAIAGEAKVP
                     FFTISGSDFVEMFVGVGASRVRDMFEQAKKHAPCIIFIDEIDAVGRHRGAGLGGGHDE
                     REQTLNQLLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVPLPDVRGRE
                     QILKVHMRKVPLGDNVKPSVIARGTPGFSGADLANLVNEAALFAARGNKRSVTMEEFD
                     RAKDKIMMGAERRSMVMTEEEKRNTAYHESGHAIVGLSVPEHDPVYKVTIIPRGRALG
                     VTMFLPENDRYSTSKRQLESRIATLFGGRVAEELVFGADAVTTGASNDIERATELARN
                     MVTRWGLSDRLGPQTYSEESGEVFLGRSVTQHKQVSDVTAHVIDEEVRRVIDSNYQRA
                     YKILQTNMDKLQTMSEALIKYETIDEDQIKDIMEGRAPRPPRDWDDTPTPTPRGPSAE
                     KPAPPLVNPAGQH"
     gene            6197..>6457
                     /locus_tag="VGE92_13530"
     CDS             6197..>6457
                     /locus_tag="VGE92_13530"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005103946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY1314897.1"
                     /translation="MQWRCRDRTIDLRRPVVMGILNVTPDSFSDGNRYTGIDAALERA
                     LQMAGEGATLIDVGGESTRPGAAAVEAAVEIARVVPVIEGICA"
BASE COUNT         1176 a         1902 c         2165 g         1214 t
ORIGIN      
        1 gggctatgtg gccgccgcgc tgctcgcggc cattgaatgg ggtttgaagc ccgtcatggc
       61 gcgcgcgcag gagcgcggca cggcgttcgg actcgggttc ggggcgtggc acggggtgtc
      121 ggcgctcctc tacctgctgg cctgcctcgc cgtcgctgta ctcctctgga aggacagccg
      181 cgtccccgcc tagttcgctt tcaggccata cacctcgagt tcgcccagga ccgatgcggt
      241 gccggtgccg atatcattgc cgcgggtgcc cacgtagacc ttgccgttgg cgacggtcgg
      301 gaccgtaaat ttgaccgcat tgcccgcctg atcggcgggc accatggagc tgttccagag
      361 ttcgctggtc aggttgccgg catccatcgc atagagcacc gccggcccgc agttcgagga
      421 ctgctgggtg caatacgggt ggctatcgat ggcccaggcg acgccgttgt tggttcccgc
      481 ggcggaaatc gtcggcgagg cgccgggcca accgtacaca tggtggctga tcgaggaggc
      541 cccgaccgca aattgccctg caaccgggtc gaaagtgtag gtctgaaggc ccgaccccga
      601 gcccgccaca tacagggtgt tgttccagaa gcctgcggtc gagaacagtc cgtggcccaa
      661 gctgaattcc tgatggacgt ttgaattgag cggcgtcgcg ttggcaccgt aatgcccgag
      721 cgagctctgc gagagcacgt acacgatccc ttcctttccg gcgacgacaa cgtagccggc
      781 gctcggcgta ttcatcagca aggccgcgcc gcccgaacca tggtcacgat cggcggagtc
      841 gagcgcggct tcatcggcgg gcgtgaacca gtcggccacg gcgagaccgc tggcggtgct
      901 cagcttgagc gtgctatcgc cgaaattggc gccgccggta tctgcgtcga acacgccgtt
      961 gccggtcgag aaatacaggt tgccaccggc gtcaaccgtg ggcgcgccgc cgctcatcca
     1021 gatgccgccg cgagcatacg tatacccgct gacttggttg ggcgaggtgt tgaagacggc
     1081 gttcggcacg agcgccaagg tcgacgcgtt gaagcccatc acccagccgt gatagggatc
     1141 ggtatcctcg tgcgatgccc aagcgatata gacggttccg ttcaccagcg tgaggccggg
     1201 ccgctgaaat tcggtttgcg gattgaagac gacctgcgaa ttgctgttgg cgtcgaccgc
     1261 ggtgccggtg acgtagatcg agccgtcgat gacggttgga ccgccggcgg tttcggtgcc
     1321 atcggtcaga cggagcgcgt gcaatcgttg atagatgggc agggcgttcg cgaccgcgga
     1381 cttgctgacc acatacagcg tgttcgtcgt cgggtcgatg accggagttc cggtgatgcc
     1441 gatttcgggc ttgacgtcgc cgtagccgct gccgacgaga ctggtcgggc cgcccgacgg
     1501 aaccgaggtt tcattcggcg tggcgccgtg actggcatcg agcagcgagg cctgccacag
     1561 agtgacgcac gggctcgcgt ccgcatcgaa ggcgaagacg ctgtcgtgct gcgtggcgac
     1621 gatgatgacg ttgtgttgcg cgccgttgat ggtcaagttg gaaatccaca gcggctgggc
     1681 gtagatcgcg ccatcgactt ggcaggagaa caatttgccg aaactggcag tgttcacatt
     1741 ggccttcgtc agcgcgtatt cctgagtatt ggcgccgtcg cgcgagttgt cgttatggaa
     1801 ggtgccgacc aggttcaagt cggtgacgcc caaggtggct gtggcgctct tggtgctgtc
     1861 cgcgacactg gtagcggtga tggtgacctg gccggcggtg ttgggcgcca catagctcgc
     1921 cgtcgaggaa gtctgcgcgc tgaaagttcc cgcgctcgcg ctccaggtga cgcctgcggc
     1981 gcccacatcg ttggcgaccg ccgccgtgag cgtcagcgtt tgcgagatcg tcaggccgcc
     2041 gcgcaccggc gtgactgtca ccgccacggc gttcgcggtg gtggtgctga cgatgtttga
     2101 atagccgctc aagtttcccg cggcatcggt cgcgcgtacc cggtaggagt acgcggtggc
     2161 ggccgtcact gcggcatcgc tccatgtcgg cgaattggcg gtggcgactt gtgcaaagtt
     2221 gctgcagccg gcgccctggc agcgctcgac gaggtagccg gtgacgccga cgttgtcggt
     2281 ggcggccgtc cagacgagag tgacctgcgt gctcgagacg agcgtcgcgt tcaagttggc
     2341 gggcgcggag ggcggcgtcg tatcgggcgg cagcggcgtc atcgccgagg cgatgcccga
     2401 gtaagcgctc agattgccgg ccgcgtcggt ggcgcgcacg cgataggaat aggacatggc
     2461 gccggtcaag ctgctatcgg tgagcgtcgt cgccgccgta gcggcgattt gcgcaaagtt
     2521 cgtgcagccc gctccttgac agcgttcgac caggtaaccg gttacgccga cgttgtcagt
     2581 agaggcagtc caggacaagt tgatctcggt gctcgagacc gcgctgctgg cgagagcgct
     2641 cggtgccgtg ggcggcgccg tatcgggcgg cagcggcgtc gtcgccgaga cgatgcccga
     2701 gtaagcgctg aaattgccgg cggaatccgc ggcgcgcacg cgatacgaat aggacaggga
     2761 gccggtcagt ccggtatcgc tccaggtggt cgagatcggt gcggcgattt gcgcgaagtt
     2821 cgtgcaaccc gccccttggc agcgttccaa ctgatagccc gcgacgcccg cgtcatccgt
     2881 ggacgcggtc caggcgaggt tgatttgaat gctcgatacg accgttgccg tcaagccgcc
     2941 gggtgccgtc ggcggcgtgt cggcgagtgg tttcgcgggc gattgcgagc cggaactgga
     3001 gccacagctg gcaaggagcg ccgagatggc gagtaggatg acgcgagcct gcaaattgat
     3061 tctaaacacg aagcctcgac ctcgacgtga caatgggtcg ggctagtctt caagcgattg
     3121 aaccttctgt gtttgcgaac caacataatc acgattcggc tgttcggaat tgtgagttgt
     3181 caccggacat aatgtcgagc gcccgcgcgg gcgcgctgcg attcaatcgg ggatgaggat
     3241 cttcggcagt tctgcgcggc ggcggtagaa gaccccgaca tggccgatgc gctgcacgag
     3301 ttcggcgtga gtacggcccg caagcatcgc cagcgttgcg ttacgcgcgt cgcgcgcgcc
     3361 gatccgggcg ctcactttga cgagttcgtg atcggtgagc gcacggtcca attcactcac
     3421 gaccccgtcg gtgagtccgg cgttgccgag catcacgatc ggactcatcg gatgcgccag
     3481 gcggcgcaaa tgtttctttt gtttctcaga cagtgccatc gcgggattat agtcataacc
     3541 cccatgccga agcgttcgaa aagcagttcc cgctggctcg ccgaacacgc cagcgacgaa
     3601 ttcgtgaagc gtgctcagaa agaaggctgg cgctcgcgcg cagtgttcaa gttggcgcag
     3661 atccaagaga gcgaaagatt gctgcgaccc ggaatgcgct gcgtggactt gggtgccgcg
     3721 ccgggcggct ggagtcaata cgccgcgcgc atcatcggcg gttcgagtcg catcgtggcg
     3781 acggacattt tggccatgga tgccatcccg ggcgtcgatt tcgtgcaggg ggattttcgg
     3841 gaaaccgccg ttttggagca ggttctcgac tgcgtgggcg ccgacaaagt ggaccttgtt
     3901 ttgtcggata tggcccccaa catggcaggc atagacgcgg tggatcagcc ccgatcgatg
     3961 tatttggcgg agttggcgct ggagtttgcc gaccgcgtgc tggcgccggg aggcgatttg
     4021 cttttgaagt tgtttcaagg tgccggcttc gatcagatca tccgggatac ccgtaaacgc
     4081 tacgcacggg tggtgatgaa aaagccgaag gcgtcacgaa cgcgcagccc tgagatctat
     4141 ctgttggcgc ggcagtttcg gatggtgtaa gttcgggagg cccggtggcg aatcttcgcc
     4201 cagccggatt gtccaatggc ggcggtaggt gaaacttgaa cgatttgacg aaacagattc
     4261 tgctttgggt cgcattggcg gtgattctgc tcgccatatt cagccaattc ggcatgcgca
     4321 ccggccagcc atccaccatc gattattcgc agtttctctc ggaagtaaag agcaataatg
     4381 tcgattcggt gaccctcaaa ggggaaatca tcgaaggcaa gcgacgctcc tcgggcgagt
     4441 cgttcgtgac ctataacccc gagaccagca atactgcgct catcggcctg atgcagtccc
     4501 agggcgtgaa attccgcggc gagccgccga aacagcagtc ggtatttctg caactgctga
     4561 tatccgcatt cccgatattg attctcattg gcttctgggc ctacttcatg cgccagatgc
     4621 aaggagcgtc cggcgggcgg ggcgccatgt ccttcggcaa gagccgcgca cggctgttgg
     4681 gcgaggatca ggtcaacatt acatttgccg atgtcgcggg aatcgaagag gccaagcaag
     4741 aagtggtcga aatcgtcgat ttcttgaaag atcccggcaa gttccagaaa cttggcggca
     4801 agatcccgaa aggcgtcctc atggtcggtt cccccggaac cggcaagact ttgctcgccc
     4861 gggccatcgc cggcgaggcc aaagtgccgt ttttcaccat ttccgggtcg gatttcgtgg
     4921 aaatgttcgt cggcgtcggc gcttcgcgcg tacgcgacat gttcgaacag gccaagaagc
     4981 acgccccctg catcattttc atcgacgaga tcgacgccgt cggccggcat cgcggcgcgg
     5041 gcttgggcgg cggtcacgac gagcgcgagc agaccttgaa ccagttgctg gtcgagatgg
     5101 acggcttcga gggcaatgag ggcatcatcg tcatcgccgc caccaatcgt cccgatgtgc
     5161 tcgatccggc gctgttgcga ccgggccgct tcgatcgtca ggtcgtggtg ccgctgccgg
     5221 acgtgcgcgg ccgcgagcag atcctcaagg tgcatatgcg caaagtgccg cttggcgata
     5281 acgtcaagcc ctcggtcatc gcgcgcggca cgccgggatt ctccggcgcg gacttggcaa
     5341 acctcgtgaa tgaggcggcg ctgttcgcgg cgcgcggcaa caagcgctcg gtcacgatgg
     5401 aagaattcga ccgcgccaag gacaagatca tgatgggagc ggagcgccgc tccatggtca
     5461 tgaccgaaga ggagaagcgc aataccgctt atcacgaatc gggtcatgcc atcgtggggt
     5521 tgagcgtgcc cgagcatgat ccggtctaca aagtgaccat catcccgcgc ggccgagcct
     5581 tgggcgtcac gatgttcctg cccgaaaacg atcgttacag caccagtaag cgacagctcg
     5641 agagccgcat cgcgacgctc ttcggcggtc gagtcgccga ggaattggta ttcggcgccg
     5701 atgcggtaac caccggcgct tccaacgata tcgaacgcgc cacggaactc gcgcgcaaca
     5761 tggtgacgcg ctggggcttg tcggaccgtc tcggtccgca gacctatagc gaagagtcgg
     5821 gagaagtatt tctcggccgc agcgtcactc agcacaaaca ggtctccgac gtcaccgcac
     5881 acgtgatcga cgaagaagtg cggcgcgtga tcgacagcaa ctaccagcgc gcctacaaga
     5941 ttttgcagac caacatggac aagctgcaga ccatgtccga ggcattgatc aagtacgaga
     6001 cgatcgacga agatcagatc aaggacatca tggaaggccg ggcgccgcgt ccgccgcgcg
     6061 attgggacga tacgccgacg ccgacgccgc gcgggccttc ggctgaaaag cccgcaccgc
     6121 cgctcgtcaa tcccgcgggc caacactagt cgccgcgttc ccttggccgc cgtgaatccg
     6181 tccctggagg ccgcggatgc aatggcgttg tcgagataga accatcgatc tgaggcggcc
     6241 ggtggtcatg gggatcttga acgtcacccc ggactccttc tccgacggca atcgctacac
     6301 cggcatcgac gctgcgctgg aacgcgccct gcaaatggcc ggcgagggcg cgactctgat
     6361 cgatgtgggc ggggaatcaa cgcggccggg cgcggccgcg gtcgaagcgg ccgttgagat
     6421 cgcgcgcgtc gtgccggtca tcgaaggcat ttgtgcc
//
LOCUS       DASWZT010000473        11343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_71516, whole genome shotgun sequence.
ACCESSION   DASWZT010000473 DASWZT010000000
VERSION     DASWZT010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11343
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71516"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1483
                     /locus_tag="VGE92_13535"
     CDS             <1..1483
                     /locus_tag="VGE92_13535"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEY1314898.1"
                     /translation="IVRGSRTPSHAFPIIDHHRAKNFVDFDEDLQVKDLENAAQEGFD
                     SSELLKRYSTVGMGPSQGKHSNMNALRILARARGVGVEQLGLTTARPLYHPVPLKLLA
                     GRSFYPERRTPIDAQHAASNAVWMPAGNWRRPEYYAVSGESRTQSIDAEVHAVRSAAG
                     LIDVGTLGKIEVYGPDAGKLLDRAYAGRFSDLKVGMTRYGLMLDESGVIVDDGVIARL
                     ADELFYFTTTTGGSASVFRELLRLNALWGLNCALVNVTGHRAAFNFAGPLSRGILQGM
                     TDIDLTEEAFPFLAVRSGNIAGAAVRLMRVGFVGELGYEVHVAAGRGPEVWQALLAAG
                     AAAGLKPFGVEAQRVLRLEKGHFIVGQDTDGLTDPLEANAMWAVAMKKPFFVGQRSLR
                     ILQARGPRQKLVGIELAAAAPLPKECHLIIEQGAIAGRVTSVTRSRALNKTIGLAMLS
                     PALAEIGRTLAIRIDDGAMVAARIAAAPFYDPKNLRQKPGLAA"
     gene            1480..2037
                     /locus_tag="VGE92_13540"
     CDS             1480..2037
                     /locus_tag="VGE92_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314899.1"
                     /translation="MSALTLQLQRRRDRLGLKGPRAAEWLVVQGIVVPMAANSWTHTT
                     DLSANDALLVARLGTTEFFLEEGTGGTMLKQIAPSLNSHLPGVYPVLREDWAFQLTGE
                     RANEVLAQVCNVNFAALAGDSQPLVMTLMIGVAVLVVPQGVGSDAVGRHYRIWCDPTF
                     GPYLGESLGAVVVECGGRYTGDDVR"
     gene            2034..3368
                     /gene="glnT"
                     /locus_tag="VGE92_13545"
     CDS             2034..3368
                     /gene="glnT"
                     /locus_tag="VGE92_13545"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III glutamate--ammonia ligase"
                     /protein_id="HEY1314900.1"
                     /translation="MNQQQAKKFFEEHKTRYVLAQFVDIHGAAKAKAVPVEHLEMVLT
                     DGAGFAGFALWGFGMGPEGPDYMAVGDLATLQDIPWMPGYARLICNGVVNKKPYAYCS
                     RVALQAQIARLTERGFTMYTGIEPEFMLLTRRADGSLGPVDPTDTLDKPCYDYKGLYR
                     SREVLDEMVAGLRAVGIDVYQIDHEDGNGQFEINFTYADVLKSADNFTFVKMAASEIA
                     HKHGMTATFMPKPFANRAGSGAHFHVSVGTAKQKNAFYDKNDKSGMGLSSMAYHFLGG
                     VLQHARALCAVAAPTVNSYKRLVVGRALSGATWAPAYIAYGDNNRTACVRIPHGRLEV
                     RLPDAGCNAYLVSAALIAAGLDGIDRKLDPGAPQNINLYQLSLEELAAKGIKLLPQSL
                     HEAIDALEADAVICNGLGPDLAREFIRLKRMEWTEYSRHVSDWESHRYLEFF"
     gene            3391..4287
                     /locus_tag="VGE92_13550"
     CDS             3391..4287
                     /locus_tag="VGE92_13550"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEY1314901.1"
                     /translation="MCGIVGLLVKKPALRSQLGALMVPMLVGMTERGPDSAGLAVYTA
                     PVSERQHKLSLYSGEHAVDWPDLLDKISSEFAQASEIAAHGNHAVLTSAADADELVAW
                     LARHAPVIAVLSVGQSIDLYKDVGAPADIAERYRFKSLQGSHLVGHTRMATESAVTPA
                     HAHPFTAGRDFCLVHNGSLSNPHLVRKRLEPLGIEFETDNDTEAACRFFEWRLREGDD
                     LKSAVQRGFSELDGFFTFLMGTDQELLIVRDAFACKPAVVAETDDYVAIASEFRSLAH
                     LPDISQATLFEPKPEEIYSWRI"
     gene            4275..4985
                     /locus_tag="VGE92_13555"
     CDS             4275..4985
                     /locus_tag="VGE92_13555"
                     /inference="COORDINATES: protein motif:HMM:NF013645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314902.1"
                     /translation="MANLRPSLRVRQFDLAKDSLRELNFFLHRQASDAGVSQIQVKNP
                     DGAHSIACGLNADLDVQIHGHVGYYVAGMNKYASVTVHGNAGPGAAENMMSGRVHVRG
                     FASTAAGASAHGGLLIIDGDASLRCGISLKGADIVVGGSVGSFSAFMAQAGRIVICGD
                     AEDALGDSLYEAVIYVRGKVRSLGADARFEPMTDTDRATVKNLLAQAGIEHDPAEFKR
                     VASAKSLYHWHADANQEY"
     gene            4988..6328
                     /locus_tag="VGE92_13560"
     CDS             4988..6328
                     /locus_tag="VGE92_13560"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEY1314903.1"
                     /translation="MTGLDLEESAGFDRSTIEYIQRAAASGLYEIRGLGAKRRVPNFD
                     DLLFLAASLSRYPLEGYRERCATKTLLGTRYATRPIELQIPITVAGMSFGALSARVKD
                     ALGRAATEVGTSTTTGDGGMTTEERASSKTLVYQCLPSRYGFDPDDVRRADAIEIVIG
                     QGAKPGGGGMLLGQKVNPRVARMRTLPEGVDQRSASRHPDWTGPDDLVIKIKELREIT
                     DWEKPIYVKVGATRVFNDVKLAVHAGADVIVVDGMQGGTAATQTVFIEHVGIPTLAAL
                     RQAVDALDDLNMRGTVQLVISGGIRSGADVAKALAMGADAVAIGQGVLVALGCNSESY
                     IAGGQHVSALEDYARLGTAPGFCHHCHTGKCPVGVTTQDAALELRLDPAVGAKRLKNY
                     LKTVNMELTTIARACGKQDVHHLEKEDLVALTVEAAAMAGIPLAGTSWIPGREG"
     gene            complement(6336..7919)
                     /locus_tag="VGE92_13565"
     CDS             complement(6336..7919)
                     /locus_tag="VGE92_13565"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY1314904.1"
                     /translation="MPDNEKDLTRREFVANAGTATLGALAAASVPLIVPRRVLGGTGH
                     RAPSDAVNLAVVGFGSMGSQNALALAPTEHIGAICDVDLAYSEQQMGERLTHDGKAQP
                     EGLKLQEQFRKARRYSDFRVMLAQEKHIDGVVIATPDHLHAVIAKAAMDLGKHVYVQK
                     PLTFSVHEARVLGQTALANPKLVTQMGNQGHSSEGARLINEWIQAGIIGAVHDVHVWT
                     NRPIVYWPQGLPRPSAAPVPDSSHPFGNPWTYGHVQDVLATAMGAGGSPPPGLDWSLF
                     VGPVAEDVPYHPIYHPFNWRGWTDFGVGAIGDMGAHLIDHPFWALGLDFPLSIEATST
                     AWGTMRLPPLDASAPANTAAGSARRQLVSYPISTSVHYQFAARGAQPPVKLHWTDGGI
                     LPSRPDGLPDEVDLRSDGGVIFVGDKGLLVHGTYGSNPQLYPHSLMDQAALVPKSIPR
                     IEWSHELNWAKAIRGEAKASSPLEYAARLTETMLLGLVALRAGQGRKILYDAERGQIT
                     NLPAANQYLTREYRAGWAI"
     gene            complement(8003..9130)
                     /locus_tag="VGE92_13570"
     CDS             complement(8003..9130)
                     /locus_tag="VGE92_13570"
                     /inference="COORDINATES: protein motif:HMM:NF005313.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1314905.1"
                     /translation="MAAVGKILIVGGSVAGMSCAIQMRKAGIDVDLVEIDPTWRIYGA
                     GITITGPTLRALRTVGVLDQVVAEGATWNGALVHDKSGKLLESVAFPSLADDLPAVGG
                     VMRPVLHRILSTRTRADGVNVRLGVTVDELRDRGSEIEVRFSDGQSGRYDLLIGADGI
                     FSKTRAQVFPDAPRPTFTGQVVYRLVAERPPGFDRSWFFMGEDYKVGFSPVSATHMYM
                     FLLHPAASNPWIEPQDQPQKLYEAMDGFGGFVPQIRATVRTTNAASVNYRPLEVLLHP
                     APWYRGRVVLIGDAAHATTPHLASGAGMAIEDGIVLSDEIQSCTSLDEALQRFMVRRF
                     ERCRMVIDNSVKLGRIEMTHGSPLEHARLMSETLGSLRQPI"
     gene            complement(9233..10165)
                     /locus_tag="VGE92_13575"
     CDS             complement(9233..10165)
                     /locus_tag="VGE92_13575"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY1314906.1"
                     /translation="MRYHKLDLNLLTALKALLAEKNVTRAGEAVHVTQSAMSGILARL
                     RDYFGDPLIVQVGRKMELTSLAESLVEPVNDVLLRIDATIATRPEFNPRSTRRHFSLV
                     ASDYSINVLLLDVLRRVHREAPGMSLEFRTPSESAAAELEAGEVDFIVNPESQNSAVQ
                     SGAILFEDSYAIVVDAGNKEVGESISLEQYLASRHVAFQSGKHGLPQFETWMENRHSE
                     ERRVEVVAHSFYLLPQLVIGTTRLATMHTRLARQLAGGLPIRLVKPSFEIPRLIEMLQ
                     WHKYRDLDPGSMWLRDVILEGARNLPPLAAIELT"
     gene            10305..11270
                     /locus_tag="VGE92_13580"
     CDS             10305..11270
                     /locus_tag="VGE92_13580"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY1314907.1"
                     /translation="MNLVITGANGFIGRALTQRLLGEDAAAPGGLGAHGRLTLLDLGF
                     EGPEDARVRRLPGSIADPGLVARAFDAPVDVLFHLASIPGGMAEQNYELARQVNLDAT
                     LSLMEAARTQMLATAKPVTVVFASSIAVLGAPLPAVVDDATPLRPRLTYGAQKLIGEI
                     LLEDFSRRAWLDSRCIRLPGIVARPPQRTGQLSAFLSDIIQELAAGRTFACPVAAEST
                     TWLMSVHRVVDNILHAARLSPAGLARRVWTLPALRTSMSQLVAAIASLYGREVLERVS
                     YGSNAALEANFGRYPPLKTPAAEEAGFAHDGDLATLVRRALLPIQ"
BASE COUNT         2008 a         3470 c         3789 g         2076 t
ORIGIN      
        1 catcgtgcgc ggcagccgca ccccgtcgca tgcctttccc atcatcgatc accaccgagc
       61 caagaacttc gtcgatttcg acgaggacct gcaagtcaag gatctcgaga acgcggcgca
      121 ggagggattc gattccagcg agctcttgaa gcgctacagc accgtcggga tgggcccctc
      181 gcagggcaag cattccaata tgaatgcgct gcggattctg gcgcgcgccc gtggcgtcgg
      241 cgtcgagcaa ctgggtctga ccacggcccg gcctttgtat cacccggtgc cgctgaagct
      301 tctggcgggc cgcagtttct atccggaacg gcgtacgcct atcgacgccc agcatgccgc
      361 gtcgaacgcg gtgtggatgc cggcgggaaa ttggcgcaga ccggaatatt atgcggtgag
      421 cggcgagagc cggacgcaga gcatcgatgc cgaggtgcac gccgtacgca gcgccgccgg
      481 cctcatcgat gtggggacgc tcggaaaaat agaagtgtac ggtcctgacg cgggcaagct
      541 gctcgaccgc gcatatgccg gccgtttttc cgatctcaag gtcggcatga cgcgctatgg
      601 gctgatgctg gatgagtcgg gcgtcatcgt cgacgacggt gtgattgcgc gattggccga
      661 cgagctgttt tatttcacga ccacgaccgg cggctccgct agcgtgtttc gggagctgct
      721 gcggctcaat gcgctgtggg gtctgaattg cgccttggtc aatgtgaccg ggcatcgcgc
      781 ggcatttaat ttcgcaggcc cgttgagccg cgggatattg caagggatga cggacatcga
      841 cctcacggag gaggcattcc cgttcctggc cgtgcgctcg ggaaatatcg cgggcgccgc
      901 ggtgcgcttg atgcgggtcg gcttcgtcgg cgaattggga tacgaggtcc acgtggccgc
      961 cggacgcggc cccgaggttt ggcaggcgtt gctcgcggcc ggtgcggccg cgggcctcaa
     1021 gccgttcggc gtggaggcgc aacgcgtgct gcgcttggaa aaaggacatt tcatcgtcgg
     1081 ccaggacacc gacggcttga ccgacccgct cgaggcgaac gccatgtggg cggttgccat
     1141 gaagaagccg ttttttgtcg ggcagcgcag tctgcgcatt ctgcaggcac gcgggccccg
     1201 ccagaagctc gtggggatcg aactggccgc ggcggcgccc ttgcccaagg aatgccattt
     1261 gatcattgag caaggcgcga tcgccggtcg cgtcacgagc gtcacgcgct cgcgtgcatt
     1321 gaacaaaacc atcggacttg ccatgctgtc cccggcgctt gccgagattg ggcgcacgct
     1381 tgcaattcgc atcgacgacg gcgcgatggt ggcggcgcgc atcgcggcgg ccccgttcta
     1441 cgatccgaag aatctgcggc agaaacccgg cttggccgca tgagcgccct gaccttgcaa
     1501 ttgcagcggc gccgcgatcg gctcggcctc aaaggaccac gcgcggccga atggctggtg
     1561 gtccaaggca tcgtcgtgcc gatggccgct aattcttgga cgcataccac ggacctctcg
     1621 gccaacgacg cgctcctggt ggcgcgtctc ggaacgacgg agttttttct cgaagaaggc
     1681 accggcggga cgatgctcaa gcaaatcgcg ccgtccctga attcgcacct ccccggcgtg
     1741 tatccggtgc tgcgcgagga ctgggccttt cagctcacgg gcgagcgggc gaacgaggtg
     1801 ctcgcgcaag tctgcaatgt gaatttcgcc gcgctggcgg gcgactccca gccgctggtc
     1861 atgacgctca tgatcggggt tgcggtactg gtcgtcccgc agggggtggg gagtgacgcg
     1921 gtcgggcggc actatcgcat ctggtgcgat ccgacgttcg gaccgtacct gggtgagtcc
     1981 ttgggcgcgg tggtggttga atgcggtggt cggtacacgg gagatgatgt gcgatgaatc
     2041 agcagcaggc gaagaaattt ttcgaggaac acaagacccg atacgttctg gcgcagttcg
     2101 tggatatcca tggcgctgcg aaggcgaagg cggtgccggt cgaacatctg gagatggtgc
     2161 tcacggacgg cgcgggcttc gccggcttcg ctctctgggg tttcggcatg ggtccggagg
     2221 ggccggacta catggccgta ggcgatcttg cgaccttgca ggacattcca tggatgcccg
     2281 gatatgcgcg cctcatctgc aacggtgtcg tgaacaaaaa accctacgct tattgctcac
     2341 gcgtggcgct gcaggcgcaa attgcgcgcc tcaccgagcg cggcttcacg atgtacaccg
     2401 gcatcgaacc cgagttcatg ctgctcacgc gccgtgcgga cggcagcctc ggaccggtcg
     2461 atcccaccga tacgctcgac aagccgtgct acgactacaa gggtctgtac aggagccgcg
     2521 aggtgctcga tgagatggtg gccgggcttc gcgccgtcgg catcgatgtg tatcaaatcg
     2581 atcacgagga cggcaacggc cagttcgaaa tcaacttcac ctacgccgat gtgctcaagt
     2641 ccgcggacaa ttttactttc gtcaagatgg cggcgagcga gatcgcgcac aagcacggca
     2701 tgacggcgac cttcatgccg aagccctttg ccaaccgcgc cggcagcggc gcgcacttcc
     2761 acgtgtcggt gggcacggcc aagcagaaaa acgctttcta cgacaagaac gacaagtcgg
     2821 gcatgggctt gtcgagcatg gcgtatcact ttctgggcgg ggtactgcag cacgcgcgcg
     2881 cgttgtgcgc ggtcgcggcg cccaccgtga attcctacaa gcggctggtg gtcgggcgcg
     2941 ctctgtcggg tgcgacctgg gctcccgcct acatcgcgta cggcgacaac aaccgcaccg
     3001 cctgcgtgcg catcccgcac ggacgactcg aggtccgcct gccggacgcc ggttgcaacg
     3061 cctatctggt gtccgcggct ctgatcgcag ccggcctcga cggcatcgat cgcaagctcg
     3121 atccgggtgc gccgcagaac atcaacctgt accaactttc tttggaagag ctggcggcca
     3181 agggtatcaa gctgttgccg cagtcgttgc acgaagcgat cgacgcgctc gaagcggatg
     3241 ccgtcatctg caacggcttg ggcccggatc tggcacgtga gttcatccgc ttgaagcgca
     3301 tggaatggac cgagtactcg cgccatgtgt cggattggga atcgcaccgc tatctcgaat
     3361 tcttttaggc gtggtcaaag gagctgccgc atgtgtggaa tcgtcggtct tctggtgaag
     3421 aaacctgcat tgcgcagtca actgggcgcc ctcatggtgc ccatgctcgt cggcatgacg
     3481 gagcgcggac cggattcggc ggggcttgcc gtctacacgg cgccggtgtc ggagcggcag
     3541 cacaaactga gtctctacag cggcgagcat gcggtcgact ggccggacct gctcgacaaa
     3601 atatcgtccg agtttgcgca ggcaagcgag atcgcggcgc acggcaatca cgcggtgctg
     3661 acgagcgcag ccgatgcgga cgaactcgtt gcctggctcg cacgccatgc gcccgtgatc
     3721 gccgtgctct cggtgggaca gtcgatcgat ctttacaagg atgtcggcgc gccggccgat
     3781 atcgccgagc gctatcgctt caaatcattg cagggctcgc acttggtcgg acacacccgc
     3841 atggcgaccg agtccgcggt gacaccggcg catgcgcatc cgttcaccgc gggccgcgac
     3901 ttttgcctgg tgcacaacgg ctcgctgtcc aatccgcacc tggtgcgcaa gcgattggag
     3961 ccgctcggca tcgaattcga aactgacaac gacaccgaag ccgcctgccg gttcttcgag
     4021 tggcggctgc gcgaaggcga tgatctgaaa tcggccgtgc agcgcggctt cagcgaattg
     4081 gacggattct tcacgtttct gatgggaacc gatcaggaac tgctcatcgt gcgcgatgcc
     4141 tttgcgtgca aacccgcggt ggtggccgag accgacgact acgtcgccat cgcgtcggaa
     4201 tttcgttcgt tggcgcactt gccggacatt tctcaggcga cgctgttcga gcccaagccg
     4261 gaggagatct actcatggcg aatttgaggc cgagccttcg cgtgcgccag ttcgatctcg
     4321 ccaaggacag cctgcgcgag ctgaactttt tcctgcacag gcaggcaagc gacgccggcg
     4381 tgtcgcagat tcaagtgaaa aacccggacg gcgcgcattc catcgcctgc ggattgaatg
     4441 cggatctcga tgtgcagatt cacggtcatg tgggctatta cgtcgcggga atgaataaat
     4501 acgcctcggt cacggtgcac ggcaacgcgg gtcccggcgc cgcggaaaat atgatgtcgg
     4561 gccgcgtgca tgtccggggc ttcgcttcca cggctgcggg cgcctcggct cacgggggcc
     4621 tcttgatcat cgacggcgac gcgtcgctgc gttgcgggat ttcgctcaag ggggccgaca
     4681 tcgtggtggg cgggagcgtc ggcagcttct ccgcgttcat ggcgcaggca ggccgcatcg
     4741 tgatttgcgg cgatgccgaa gatgcgctcg gcgactcatt gtacgaggcg gtgatctacg
     4801 tacgcggcaa ggtgcgctcg ctcggcgccg atgcccgctt cgaaccgatg accgataccg
     4861 atcgcgccac ggtcaagaat ttgctggcgc aggccggtat cgagcacgac cccgcggagt
     4921 tcaagcgcgt ggcgtccgcc aagagtctgt atcactggca tgccgatgcc aatcaggagt
     4981 attgagaatg acgggccttg acctcgagga atcggcgggt ttcgaccgat ccacgattga
     5041 gtatattcag cgcgccgcgg ccagcggcct gtacgagatc cgcggcttgg gcgccaaacg
     5101 ccgggtgccg aactttgacg atttgctgtt tctggcggcc tcgttgtcgc gctacccgct
     5161 cgagggttat cgcgagcgct gcgccaccaa gacgctgctc ggcactcgat acgcgacacg
     5221 cccgatcgaa ctgcaaatcc ccatcaccgt cgccggcatg agcttcggcg cactctcggc
     5281 ccgcgtgaag gatgcattgg ggcgcgccgc caccgaagtc ggtacttcga caaccaccgg
     5341 tgatggcggt atgaccaccg aagagcgggc ctcgtcgaaa acgctggttt atcagtgcct
     5401 gccgtcgcgc tacggcttcg atccggatga cgtgcgtcgg gccgatgcca tcgaaatcgt
     5461 gatcgggcag ggggcgaagc cgggcggcgg cggcatgctg ctcggccaga aagtgaatcc
     5521 gcgggtggcg cgcatgcgca cgctgccgga gggcgtcgat caacgctcgg cgtcgcgcca
     5581 tccggactgg accggacccg atgatctggt catcaagatc aaggaattgc gcgagatcac
     5641 cgattgggag aagccgatct acgtgaaggt cggcgccacg cgcgtgttca atgacgtgaa
     5701 gctcgcggtt cacgccggcg ccgacgtcat cgtcgtcgac ggtatgcaag gcggcaccgc
     5761 cgccacccaa acggtgttca tcgagcacgt cggaattccc acgctcgcgg cgctgcgcca
     5821 agcggtcgac gcgctcgacg atctcaacat gcgaggcacg gtgcaattgg tgatttccgg
     5881 cggtatccgc agcggcgccg atgtcgccaa ggcgctcgcc atgggcgcgg atgcggtggc
     5941 catcggtcag ggcgtgctgg tggcgctcgg ctgcaacagc gagtcgtaca ttgccggcgg
     6001 ccagcatgta tctgcgctcg aggattacgc gcgtctcggc acggcgcccg gcttttgcca
     6061 tcactgccac accggaaaat gtccggtggg ggtgacgacg caggacgcgg cgctggaact
     6121 gcgcttggac cccgcggtcg gcgccaagcg actgaagaac tatctgaaaa ccgtgaacat
     6181 ggaattgacc accatcgcac gcgcctgcgg taagcaggac gtgcatcatc tcgagaagga
     6241 ggatctcgtg gcgttgacgg tcgaagcggc cgccatggcg gggattccgt tggcggggac
     6301 ttcctggata ccgggccgag agggttagaa gcgcgtcaga tggcccagcc ggcgcgatac
     6361 tcgcgcgtca agtactggtt cgcggcgggc aggttggtga tctgtccgcg ttctgcgtcg
     6421 taaagaattt tccttccctg gccggcgcgc agcgcgacga gtccgagcag catggtctcg
     6481 gtcaggcgcg cggcatattc caatggcgaa ctggccttgg cctcgccgcg aatcgccttg
     6541 gcccagttga gctcatggct ccactcgatg cgcggaatac tcttgggaac cagggccgct
     6601 tggtccatca gcgagtgcgg gtagagctgc gggttcgagc cgtaggtgcc gtgcaccagc
     6661 aagcctttgt cgccgacgaa gatcacgccg ccatcgctcc tgagatcgac ctcgtccggc
     6721 aagccgtcgg gccgggaggg caaaatccca ccgtcggtcc agtgcagctt cacggggggt
     6781 tgtgcgccgc gcgcggcgaa ttggtaatgc acgctggtgg agatgggata cgacaccaat
     6841 tggcgcctgg ccgagccggc cgcagtgttg gccggcgcgg aggcatcgag gggcggcagg
     6901 cgcatcgtgc cccaggcagt cgacgtggct tcgatgctta aggggaaatc gaggcccaat
     6961 gcccagaagg ggtggtcaat caggtgcgcg cccatgtcgc cgatcgcgcc gacaccgaag
     7021 tcggtccagc cgcgccaatt gaaagggtga tagatcggat gataggggac atcctcggca
     7081 acggggccca caaaaagact ccagtcaaga ccgggcgggg gcgacccgcc ggcccccata
     7141 gccgtggcga gcacgtcttg aacatggcca taggtccagg gattgccgaa ggggtgggac
     7201 gaatcgggca cgggcgctgc gctggggcgg ggcaagccct gcggccagta gacgatcggg
     7261 cgattggtcc agacgtgcac atcgtggacg gcaccgatga tgccggcctg gatccattcg
     7321 ttgatgagtc gcgcgccttc gctcgaatgt ccctggtttc ccatctgagt cacgagcttc
     7381 gggttggcga gtgccgtctg gcccaacacg cgggcttcgt gcaccgagaa cgtcagcggc
     7441 ttttgtacgt acacatgctt gcctaggtcc atggcggcct tggcgatcac cgcatgcaag
     7501 tggtccggtg ttgcgatcac cacgccgtcg atgtgcttct cctgggcgag catcacgcga
     7561 aagtcgctgt agcgtctggc tttgcgaaac tgctcttgga gcttcaggcc ttcgggctgg
     7621 gccttgccgt cgtgcgtcaa gcgctccccc atttgctgct cactgtaggc gagatcgacg
     7681 tcgcagatcg cgccaatgtg ttcggttggc gcaagagcga gggcattttg cgagcccatg
     7741 ctgccgaagc cgacgaccgc gaggttcacc gcgtcgcttg gcgcccgatg gccggtgccg
     7801 ccgagcacgc gacgcggcac gatcaatggg acgctggcgg cggcgagcgc accgagcgtg
     7861 gcggttccgg cgttcgccac gaactcgcgg cgagtgaggt ctttctcgtt gtcgggcatc
     7921 tcaccagtct accgggcgga cgcgatcgcg agaagcgatt gtccggggcc gccgcctgat
     7981 ggcggccggc cccgcgcagg cgtcaaatgg gttggcgcag cgaacccaag gtttccgaca
     8041 tcagccgcgc gtgctcgagc ggcgagccgt gagtcatttc gatccggccg agcttcaccg
     8101 agttgtcgat gaccatgcgg cagcgctcga agcggcgcac catgaatcgc tgcaaggcct
     8161 cgtccaacga tgtgcacgat tgaatctcgt cgctcaacac gatgccatcc tcgatggcca
     8221 tgccggcacc ggaggccaaa tgcggcgtgg tcgcgtgcgc ggcgtcgccg atcagcacga
     8281 cccggcctcg ataccagggc gcgggatgca gcagcacctc gaggggacga tagttgacgc
     8341 tggctgcatt ggtggtgcgc accgtcgcgc ggatctgcgg cacgaatccg ccgaagccgt
     8401 ccatggcttc gtagagtttc tgcggttgat cctgcggttc gatccaaggg ttcgaggctg
     8461 cgggatgcag caggaacatg tacatgtgcg tggccgacac gggactgaag ccgaccttgt
     8521 aatcctcgcc catgaagaac cagctgcggt cgaatcctgg gggtcgttct gccaccagcc
     8581 ggtatacaac ctgccccgtg aaggtgggtc tcggcgcgtc gggaaacacc tgcgcgcgcg
     8641 tcttggaaaa gatgccgtcc gcgccgatca gcaaatcgta gcggcccgat tggccatcgc
     8701 tgaaccggac ttcgatctcg ctgccgcggt cgcgaagttc gtcaaccgtg acgccgaggc
     8761 gaacattcac gccgtccgcc cgcgtacgcg tcgacagaat ccggtgaagc acgggccgca
     8821 tcacgccgcc gacggccggc aggtcgtccg ccaacgacgg gaaggcgacg ctttccagaa
     8881 gctttcctga cttgtcgtga accaaggcac cgttccaggt cgcgccctcg gcgacgactt
     8941 ggtccagcac accgacggtt cgcaaggcgc gcagtgtggg acccgtgatc gtgattcccg
     9001 cgccgtaaat tcgccaggtc ggatcgattt caacaaggtc gacgtcgatg ccggccttgc
     9061 gcatttgaat cgcgcaggac atgccggcga cgctgccccc gacgatgaga attttgccga
     9121 ctgcggccac gtgtctctcc ttgtcttgag tgtcagtgta gcgcgcggtt cggggcaaat
     9181 gtccgccgca agcatcgcgt ttgataatgc tcggaatcgg cgcgggagat tgttaggtca
     9241 gttcgatcgc ggccaagggc ggcaagttgc gtgcgccttc gagaatcacg tcgcgcagcc
     9301 acatgctgcc gggatcgagg tcgcgatact tatgccattg cagcatctcg atgaggcggg
     9361 ggatctcgaa cgacggcttc accagccgaa tgggcaggcc gcccgcgagc tggcgcgcga
     9421 gccgggtatg catggtggca agacgcgtgg tgccgatgac gagctgcggc agcagataga
     9481 agctgtgcgc gaccacctca acacgccgct cctcgctgtg gcggttctcc atccacgttt
     9541 caaactgtgg cagaccgtgc ttgccgctct ggaaggcgac atgacgcgac gccaggtatt
     9601 gctcgagcga aatcgattca ccgacttcct tgttgccggc atcgaccacg atggcgtagg
     9661 agtcttcgaa caggatggcg cccgactgca cggctgagtt ctggctctcc ggattgacga
     9721 tgaaatccac ctcgcccgct tcgagttccg cggcggcgct ctcgctcggc gtgcggaact
     9781 cgagcgacat gcccggagct tcgcgatgaa cgcggcgcag cacatcaagc agcaggacgt
     9841 tgatcgaata gtccgaggca accagcgaaa aatgccggcg cgtgctccgg gggttgaact
     9901 ccggccgcgt cgcgatggtt gcatcgatac gcagcaatac atcgttgacc ggctcgacca
     9961 ggctttccgc gagcgatgtc aattccatct tgcggccgac ctgaacgatc agcgggtcgc
    10021 cgaagtaatc gcgcagacgg gcgaggatgc cgctcattgc cgattgagtg acatgcacgg
    10081 cctcgcccgc gcgcgtgacg tttttctccg ccagcagagc cttgagcgcc gtcaataaat
    10141 tgaggtcgag cttgtggtag cgcatggcgc ttgattgtac gccccgggcc gcggcccgcg
    10201 ggtttccgca tgggtgaacg tgatggcggg catcgaaagg atcgatttcc gtcccgaatc
    10261 aagtcgctcc atgatgcatg aaacgggcag ccgtgagtgg gcggatgaat ctcgtaatta
    10321 caggtgccaa cggcttcatc ggtagggcgt taacccagcg cctgctgggc gaggacgccg
    10381 cggcacccgg cggcttgggc gcccatgggc ggctcaccct gctcgatctc ggattcgaag
    10441 gacccgagga cgccagagtt cgcaggcttc ccggcagcat cgccgacccg ggcctcgtgg
    10501 cacgcgcctt cgatgcaccg gtcgatgtgt tgtttcatct cgccagcatc ccgggcggca
    10561 tggccgaaca aaactatgaa ctggcccgcc aggtgaatct ggatgcgacg ttgagtctga
    10621 tggaagcggc gcgcacgcag atgctggcga cagcgaaacc ggtgaccgtg gtatttgcaa
    10681 gttccatcgc ggtgctgggc gcacccttgc cggcggtggt cgacgatgcg acaccgctcc
    10741 gtccacgctt gacctacggt gcgcaaaaac tgatcggcga gatcctgctg gaggacttct
    10801 cgcgccgcgc ctggctcgat tcgcgctgca tccggctgcc gggaatcgtc gcccggccgc
    10861 cgcagcggac cggacagttg tcggccttct tgagcgacat cattcaggaa ttggcggcgg
    10921 ggcgcacctt tgcgtgcccg gtggccgcgg agtcgaccac ctggctcatg tccgtgcatc
    10981 gcgtggtcga caatatcctg catgcggcgc gcttgtcccc ggccggcctg gcgaggcgcg
    11041 tctggacgtt gccggcgctg cgcacgtcca tgtcgcaact ggtggcggcg atcgcgagcc
    11101 tgtacggccg cgaggtgctg gagcgggtgt cgtatgggtc aaacgcggcg ctcgaagcga
    11161 atttcgggcg ctatcccccg ctcaagacgc cggcggcgga agaggcgggt tttgcgcacg
    11221 acggcgacct cgccacgctg gtgcggcgcg ctctgttacc gatccaataa tatgcaaaac
    11281 aaccccctca tcggctggac cgtggatcgt tccgtcatcc gcacgatcgg cagcggcttg
    11341 cag
//
LOCUS       DASWZT010000474        11595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_81291, whole genome shotgun sequence.
ACCESSION   DASWZT010000474 DASWZT010000000
VERSION     DASWZT010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11595
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81291"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VGE92_13585"
     CDS             complement(<1..352)
                     /locus_tag="VGE92_13585"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY1314908.1"
                     /translation="MSSVQIEQKSYEMPPREGISIAHFLTVSDIERSARYYEKVFGAR
                     ILSLGDGNAPCYLQLANIWMILNVGGGPTPDKPTVTLSVPDPNHINSFMNFRVADIQG
                     CYKLWKSRGAEFITE"
     gene            complement(472..1128)
                     /locus_tag="VGE92_13590"
     CDS             complement(472..1128)
                     /locus_tag="VGE92_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314909.1"
                     /translation="MSPGARSKTSKKCAQILLVIVASAVAAAAAQAKAPDTIVFVPPT
                     DLPALARQSGEAMFLHRTIGGRTLLYIEQDHGSRLATLDVTDPAHVRAKSSVQLDSSG
                     PFDFFAALGSNAELIRFRQGYQVGVLDLHKENAANLKVAPAMSLQGQATPVGNDVVNT
                     QTLNQVLDGKQVREEVTNATTGTTFLLTENGLYLIRRPAVESENRRRQEEWFLEHAGG
                     "
     gene            complement(1432..3036)
                     /locus_tag="VGE92_13595"
     CDS             complement(1432..3036)
                     /locus_tag="VGE92_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314910.1"
                     /translation="MAVDSHFHRNPFFVLGATTHDDRKKLAELAAARSLEFPSGVCDQ
                     ARTELTRSRTRLAAEIAWFPGVPPSAVPALIELLRTDIEAARRQADLSDLVRANLLAA
                     LWQEIPARQPTLALTDLIVETGTVIDRISAAAVMRDVNRDRAISGFGPIQSIDEVQVE
                     LAERLRFVCSLIRGALDLLPSESIIEALRRVVVAATDNGQRQASALVDELIDSYEIDT
                     RTVLEQEAAAALSLIESVRHKMARGSAGMQPFLRQLDAVVHNWDRIAQPIQLSCLARG
                     LSHQPSLDLGYAIRNFAIELWNQYRVMDESTRLILLSEKMFAEIPPLVDLLKDDRTAL
                     AGIAESLNYRTEIGRFVKHTLSISADAVQWKGQHYRLESITRIRWGAETRTFSGVVKR
                     TTYTVAFGDAERESCIETRNRDIYQNFIGKLWSTAGVRLLAEFMEILKAGGIIRFGRA
                     WFRDEGVDLVQICRFGRKQVVSCAWDQVVVKNFNGSLTVSAKDNSSAWATLSFRDTPN
                     VYVLAKALELVLQPPFKQKMSALLRQ"
     gene            complement(3056..3463)
                     /locus_tag="VGE92_13600"
     CDS             complement(3056..3463)
                     /locus_tag="VGE92_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314911.1"
                     /translation="MSKDLDLMPLGSGKAARGFAGLSALASPIDFEALEMESRATEAP
                     ASDPHIRALRFASFDSPLFSGWRTSLRRIAIMLAVPPTAALCAWLLASVMLAIRPSES
                     VDNAVPGDCADVGHIPASADARPCGSIPARPEK"
     gene            complement(3460..4833)
                     /locus_tag="VGE92_13605"
     CDS             complement(3460..4833)
                     /locus_tag="VGE92_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019751263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1314912.1"
                     /translation="MTRDSWFPIGYALPGGGSSGRCLFQGDGWQILGVATRGRALIAG
                     AELAQRWIDAALVDRDVLGVIDFGPRRYYALICGENHALSPVCDSPSPNTKAEALAFS
                     VALRATRAIDRQTPLQEAIYVEKISRVLPTYGASGGCADDVVLGRWLTGGVNISVQSF
                     QRLREIVSWLPAESLRNVIEAAGLAVSEPSPAVATASTTRSGKFELSGRPELASFFNE
                     HIVDVVQNRERYLALGIGFPGAIVLHGPPGCGKTFAVEKLSEFLGWPCYSIDAASVAS
                     PYIHETSKKVAAVFDDAKRNAPSLLVIDEMEAFLADRESGSGHHRIEEVAEFLRRIPE
                     AAKNEVLVIAMTNRIDLIDQAILRRGRFDHIVNVDYPNESEVLPLLIDLLSSLPKEAD
                     VAPAPLAAALSGRPLSDVAFVVREAGRRAARSGKNRISQADLAAALESAPSVDPEAGK
                     RNKIGFV"
     gene            complement(4839..7340)
                     /locus_tag="VGE92_13610"
     CDS             complement(4839..7340)
                     /locus_tag="VGE92_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019751264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY1314913.1"
                     /translation="MAAVNYVGIDLGTTNSVISSYDGERVHLYKSPEQQDVTPSAIFV
                     DKRGNRYLGLRAYNNAARNPDNAALNFKRLMGTSTPILLAAVNLTMTPEECSAEILKL
                     LYGYLPAAIRADQDTGVVIGVPAAFNQMQKDATMTAAQAAGIGRAALMQEPVAAVMSV
                     MRAEPRDGAFMVYDLGGGTLDVAIAESIGGRVSLLAHGGIAMCGGRDFDRAMFDQIVK
                     PWLLARFKLPFELAANPQYRPLVRMATWAAENAKIALSQRDQATISLSETDLAARDTA
                     GEEIFVDIAIDRTALDRLIASQVDASIAAAREILASSGLNPANVERIVFVGGPTQYPP
                     LRAKVSAALGIAVSTDVNPMTAVAEGAAVFAESIDWSTASHGRKTSRGVIGTGGTHAI
                     TFNFIARTPDTKARIVADMGGDVAAGYAFQIDSLDSGWTSGRMPLRDGASVDLVLAKP
                     ADNSFKVFVFDSNGSPLTLPQDRITISRTAAHIDAIPASHSIGIEVLDRPNGKPVLDF
                     LVRSGDRLPAKGKREYKAAESLRSGASGCIRIRLWEGDIADPVQDNLPIGTLKINGSD
                     IEEGAIAAGEKLIVEYEIFDSGTIDLKVSVPAIGALFRSGRNFYARQEAQIDFTKAAK
                     LIADMCSLTRSRIDQMLSKINDIRLDQARDKLDAMPSGEFGQIDAESAKKILDELQAI
                     RRLLAQVRQDHLAAMRSVELANLTHHFEKTVRTLARPSEVSAFENLAKTAQRAIDRNT
                     GDFESHVDAMRARIHEILWRQDWYVIDRFNWLVKSSYLFPDAEEHAKLAALAADAMHA
                     NDFEKFRGLVGQFDAKRLGLPSDEDLAGTVNLLRG"
     gene            complement(7328..7678)
                     /locus_tag="VGE92_13615"
     CDS             complement(7328..7678)
                     /locus_tag="VGE92_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019751265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314914.1"
                     /translation="MIEVEQALIGLAVESWRFSRAFARLARKLDIADAARYASQLDYF
                     EKTIEESLQAAALTLVNLEGHEFDPGMAVTALNLGDFSAEDRLLVDQMIEPIVMSRDG
                     LRRQGIVALRKWPR"
     gene            7985..10156
                     /locus_tag="VGE92_13620"
     CDS             7985..10156
                     /locus_tag="VGE92_13620"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY1314915.1"
                     /translation="MNTPATAEQIAQLHTTPRRRVLIVDDDAIFALLAQETLEQAGFD
                     ATIAANAAEAFAAFERDKPDLVLLDVDLPGGNGFDICAALRALNSGADVPIVMVTGHD
                     DTKSIAQAYEAGATDFIHKPVLWPTLPHRVGFTLRALDNMRALKLSEQKNRALLQALP
                     DTLFIVNSLGVLLEHITGSERKSDEWLVGKMLEEVFPAGLAQAARLSLENAGSGALTT
                     YEFGVERERDPRWYEARLRPQADGKLLIVIRDQTERRKAKARIEYLAYYDVLTRLPNR
                     QLFVREAARAVAQAKQSGQMLALLYLDLDRFKRINDNLGHAVGDALLQSVARRLEQSV
                     RQTDIVSQVGEPPKPEPMRVARLGGDEFVVLLTDVINEMQTVKIAERIQQSLAEPFEC
                     SGHRFVVTPSIGIAMFPQDATDVDDLLVKADMAMYQAKDQGRNGHAFFGQSMAVRSLG
                     RLELENDLRRAFDSGEFQIYYQPKLELASGAIIGAEALLRWLHPQRGWITPDSFIPVA
                     EETGLIIALGDWVIREACVQLAAWAKQGLGHLTLAVNVSVQQFAREDFVDTVLGALKE
                     FAVKPQQLELEITESLLMRNVDDTTACMRRFRAAGVALSIDDFGTGYSSLGYLRQFPV
                     DSLKIDRSFVKDLHRSEDDAAICAAIIAMARELKLKVIAEGVENAEQLKFLRRHRCDQ
                     VQGYLISRPIPMAELRILLQKAPQVEGMSVDFAYSAKTAGR"
     gene            10187..>11595
                     /locus_tag="VGE92_13625"
     CDS             10187..>11595
                     /locus_tag="VGE92_13625"
                     /inference="COORDINATES: protein motif:HMM:NF028461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEY1314916.1"
                     /translation="MMRWIDNLSLASKLRVMIVLAVAVVLLLASALYASVEALALRAN
                     LAQQLLILTTAAGQNAAAPLALHNRNLARNALEPLHADPTMRAVALYDAAGHIFLNFS
                     APGTKLLSDDRLKDNQALHFDGLTRTDIQVHVILDGKPVGTLRLQADLSAQFYPQFKS
                     SVQFMLVGFALAGTVAFLLSSRLRRVISMPVGNLVQVTRDVLKSKDFSIRADKISSGE
                     FGTLIDGFNEMLAELERRDRNLRAYQNELERLVRERTVQLDIEVAEAQEAAKRAEGAS
                     RAKSDFLARMSHEIRTPMNGVLGMAELLRHSTTLDERQRRYAITIHESGSALLQIIND
                     ILDFSKIEAGKLQIDIAPFCVREVVEDAVEILAERAHAKGLELICDIPTRIATKASGD
                     ALRLRQVLINLISNAVKFTEHGEVCIRVNSVDAGLHNSSFDFEVSDTGIGIKPENCAT
                     IFESFAQADVSTTRQYGGTG"
BASE COUNT         2267 a         3512 c         3528 g         2288 t
ORIGIN      
        1 gctcggtaat gaactctgct cctcgactct tccataattt gtagcaccct tgaatatccg
       61 caacgcggaa attcatgaag ctgttgatgt gattcgggtc ggggacgctg agcgttaccg
      121 tcggcttatc cggggtcggc ccgcctccaa cgttcagaat catccaaata ttcgcaagct
      181 gaaggtagca gggcgcgttg ccgtcaccca ggctcaaaat gcgagcgccg aagacctttt
      241 cgtagtagcg agccgatcgc tcgatgtcgg agaccgtgag aaaatgggcg atgctgattc
      301 cctctcgcgg gggcatctca taactcttct gctcaatttg tacgctgctc attgacgact
      361 ccaattccga ttaacagtgc gcatcggctg taagcagtta agcctaatga ggaaggaaga
      421 acaattgcac caaggtatca gctgagcgtc cgaaattcag acccagcagc gtcaaccccc
      481 tgcgtgctcc aagaaccatt cctcttgacg ccgccggttc tccgactcga ccgccggccg
      541 cctgatgagg tacagaccgt tctccgtgag cagaaacgtc gtgccggtag ttgcgttcgt
      601 cacttcttca cggacctgct tcccgtcaag gacttgattt aaggtctgtg tgtttacgac
      661 atcattgccc acaggggtgg cttgaccctg caagctcatc gccggggcga ctttgaggtt
      721 cgccgcattt tccttgtgca aatcgagcac gccgacttga taaccctgac ggaatcggat
      781 caattccgcg ttgcttccga gcgcagcaaa gaaatcaaag ggtccggagg aatcaagctg
      841 caccgaactc ttggctctaa catgggccgg gtcggtcaca tcgagggttg ccagacgcga
      901 cccgtggtct tgttcgatat agagtagcgt ccggccgcca atcgtccggt gcagaaacat
      961 tgcttcgccg ctttgccggg ctaatgcggg tagatccgtc ggtggcacga aaacaattgt
     1021 atcgggcgcc ttggcctgag cggccgctgc cgccacggcg cttgcgacta tcactaaaag
     1081 gatttgtgcg catttcttgg atgttttgct tctagcgccg ggtgacatgg cgatctctcc
     1141 tcatagctgc gggaatgact gtctccaaca acaagactcg caaccgcgca cgtttattcc
     1201 atcgccgccg cccgaacgcc gctggctgat ctttcgttgc cgcgacaaat tcgtgaactc
     1261 ggctctcgcc atggcggggc tgcgctcgct cgtcggcggc gaaattaccg agtacacgca
     1321 attgctcgag gaggcgcggc gtcacgctgt cgaccggatg gtccgcaacg cgaatctcat
     1381 gcacgccgat gccaagcgta gccggccggc cgacgcgccg ttcggcgagt gctactgccg
     1441 caacagcgcg ctcatttttt gcttgaaagg cggttgcaaa accaattcaa gggcctttgc
     1501 caatacgtag acgttcggcg tatctctgaa agacagcgtc gcccacgcgc tcgaattgtc
     1561 tttcgcgctc acggtgagcg agccgttgaa attcttgacg accacctggt cccatgcgca
     1621 gctcaccact tgcttgcgac cgaatcggca tatttgcaca agatcgacac cctcatcgcg
     1681 gaaccacgcg cgcccgaagc gaatgattcc gccggccttc aagatctcca tgaattccgc
     1741 caacaggcgc acgcccgccg tgctccagag tttcccgatg aaattctgat agatatccct
     1801 attcctcgtt tcgatgcagg attcgcgctc ggcgtcgccg aaagcgacgg tgtaggtcgt
     1861 gcgcttgaca acgccgctga aggtgcgcgt ttcggctccc cagcggatcc tggtgatcga
     1921 ctcaaggcga tagtgttggc ccttccattg cacggcgtcg gcggaaatac tcaaggtgtg
     1981 cttcacgaat cgcccgatct cggtgcggta gttgaggctc tccgcaatgc ccgccaaggc
     2041 ggtccggtcg tccttcaata ggtcgaccag gggcggaatc tcggcgaaca ttttctccga
     2101 cagcagaatc aggcgggttg actcgtccat gacccgatat tggttccaca actcgatcgc
     2161 gaaatttcgg atggcgtagc ccaagtccaa actaggttgg tgactcagtc cgcgagccag
     2221 gcagctcagc tggatgggtt gcgcgatgcg atcccaattg tggacgaccg cgtccagttg
     2281 ccgcagaaac ggctgcatgc cggctgagcc gcgcgccatc ttgtggcgca ccgactcgat
     2341 caagctcagg gccgccgcgg cctcctgctc caggacggtt cgcgtatcga tttcgtagct
     2401 gtcgatcagc tcgtcgacca gggcgcttgc ttgccgttgg ccattgtcgg ttgcggcaac
     2461 gacgacacgc cgcagcgcct cgatgatcga ctcggacggc aagaggtcta gcgcaccgcg
     2521 aatcaatgaa cacacaaatc gcagcctctc ggccagttcg acttgcacct cgtcgatcga
     2581 ctggatcggg ccgaacccgg agatggcgcg atcccgattg acgtcccgca tgacggccgc
     2641 cgccgagatc cgatcgatga cggtaccggt ttcaacgatg agatcggtca aggcaagcgt
     2701 cggctggcgc gcggggattt cctgccatag cgccgcgagc agatttgcgc gcaccagatc
     2761 cgagagatcc gcttgcctgc gggcagcctc gatgtcggtg cgcaacaact cgatgagggc
     2821 ggggaccgca ctcggcggta caccgggaaa ccaagcgatt tctgcggcga gccgcgtgcg
     2881 cgagcgggta agctcggttc gagcctggtc gcaaaccccg cttgggaatt ccaggctccg
     2941 ggccgcagcg agctcagcca gctttttgcg gtcgtcatgg gtggtcgcgc ccaatacgaa
     3001 aaacggattt cgatggaaat gtgagtcgac ggccaccggg tatgtccgcg ctcggttatt
     3061 tttccgggcg tgcgggaatg ctgccgcaag ggcgcgcgtc agccgatgcc ggtatgtgcc
     3121 cgacatcggc gcagtcgccg ggcaccgcgt tgtcgacact ctcgctcgga cggatggcga
     3181 gcatcaccga ggcgagcagc caggcgcaca gggccgctgt cggtggaacg gccagcatga
     3241 tcgcgatccg gcgcaggctg gtccgccagc ccgaaaacag cgggctgtcg aaacttgcga
     3301 agcgcagagc acgaatatgc gggtcgctcg ccggagcttc cgtggcgcga ctttccatct
     3361 ccagcgcttc gaaatcgatc ggcgaggcga gcgcggatag tccggcaaat ccgcgcgcgg
     3421 ctttcccgga gccgagcggc atgagatcga gatccttact cacacgaagc cgatcttgtt
     3481 gcgtttgccg gcctcgggat cgacgcttgg cgcgctctcc agggccgcgg cgagatcagc
     3541 ttggctgatg cggttcttgc ccgagcgtgc cgctctgcgg cccgcttcgc ggaccacgaa
     3601 cgccacgtcg gacagcggtc ggccggacag cgccgcagca agcggggcgg gagccacgtc
     3661 agcttccttc ggcagggatg acagcaggtc gatgagcagc ggcaagactt ccgactcgtt
     3721 cggatagtct acattcacga tgtgatcgaa tcggccgcga cgcaaaatag cctgatcgat
     3781 cagatcgatg cgatttgtca tggcgatgac cagcacttcg tttttcgccg cctccgggat
     3841 acggcgcagg aattcggcca cttcttcgat ccgatggtgc cccgatcccg actcgcgatc
     3901 cgcgaggaag gcttccatct catcgatgac caaaagcgag ggtgcgtttc gcttggcgtc
     3961 gtcgaagacg gccgcaactt tcttgctcgt ttcatggata taggggctgg cgacgcttgc
     4021 cgcgtcgatc gaatagcagg gccagccgag aaattcgctc aatttttcga cggcaaatgt
     4081 cttgccgcag cccggcggcc catgcagcac gatggcgccg ggaaatccga tacccaaggc
     4141 caggtagcgt tcccggtttt gaaccacatc gacgatgtgt tcgttgaaga aagacgctag
     4201 ctcaggtctg ccggagagtt cgaacttgcc ggagcgcgtg gtgctcgccg ttgccacagc
     4261 ggggctcggc tcactcaccg caagaccggc cgcctcgatc acgttgcgca gagactcggc
     4321 cgggagccag ctgacgatct cgcgcaggcg ctggaacgac tgaaccgata tattgacgcc
     4381 gcccgtcaac catcgaccga ggaccacatc gtcggcgcag ccgccgctgg cgccgtaggt
     4441 cggcagcacg cggctgatct tctcgacgta gatcgcttcc tgcagcggcg tctgccggtc
     4501 aatggcgcgc gtcgcccgca aggcaacgga gaaagccagc gcctcggcct tggtgttggg
     4561 cgatgggctg tcgcagactg gcgaaagcgc atggttctcg ccgcaaatga gcgcgtaata
     4621 ccgtcgcggc ccgaagtcga tgactccgag gacgtcgcga tcgaccagcg cggcgtcgat
     4681 ccatcgctgc gccagttcgg cgccggcgat gagcgctctg ccgcgcgttg cgacgccaag
     4741 aatctgccac ccgtcgcctt gaaagagaca gcggccgctg ctgccgccgc cgggcaacgc
     4801 atagccaatc ggaaaccagc tatcacgagt caaactgttt accctcgcaa gagattgacc
     4861 gtgccggcca agtcctcgtc gctcggcaag ccgagacgct ttgcatcgaa ttgcccgacg
     4921 aggccgcgaa atttttcgaa atcgttggca tgcattgcgt cggccgccag cgccgctagt
     4981 tttgcatgct cctcggcatc ggggaacaag taacttgact taaccaacca attgaagcga
     5041 tcaatcacat accaatcctg ccgccacagg atttcatgga ttctggcgcg catggcatcg
     5101 acgtgcgact cgaagtcgcc ggtgttgcga tcgatcgccc gctgcgccgt ttttgcgagg
     5161 ttttcgaacg ccgacacctc ggaggggcgg gcgagcgtgc gcacggtttt ttcgaaatgg
     5221 tgggtcaaat tggccagttc caccgaacgc atcgcggcca gatggtcctg gcgcacctgt
     5281 gccagtagac gtctgatcgc ctgcagttca tcgagtatct tcttcgccga ctccgcatcg
     5341 atttggccga actcgccgct tggcatcgcg tcgagcttgt cgcgcgcctg atcgagcctt
     5401 atgtcgttga ttttcgagag catctgatcg atgcgtgagc gtgtcaagga acacatgtct
     5461 gcgatcaact tcgccgcttt ggtgaagtcg atctgcgctt cctggcgcgc atagaagttg
     5521 cggccgctgc gaaatagtgc gccgattgcc ggaaccgaga ccttgagatc gatcgtgccg
     5581 gagtcgaaga tttcgtactc gacgatgagt ttctcgccgg cggctatcgc cccttcctcg
     5641 atgtccgagc cgttgatctt cagtgtgccg atcggcaggt tgtcttgcac cggatcggcg
     5701 atatcgccct cccacaggcg gatcctgatg cagccggagg cacccgatcg cagcgactcg
     5761 gccgccttat actcccgctt gcccttggcc ggcagccggt cgccggagcg caccaaaaaa
     5821 tccagcaccg gtttgccatt gggccggtcg agtacctcga taccaatcga gtgcgacgcg
     5881 gggatcgcgt cgatatgcgc ggcggtacgc gagatcgtga ttcggtcttg cggcagagtc
     5941 aggggtgagc cgttcgaatc gaaaacgaag actttgaatg aattatcggc cggcttggcc
     6001 agcacgagat cgacgcttgc gccgtcgcgg agcggcatgc ggcccgatgt ccagccggaa
     6061 tcgaggctgt ctatttggaa cgcatagccg gcggcgacat cgccgcccat atcggcgacg
     6121 atgcgcgcct ttgtgtccgg tgtgcgcgcg atgaaattga aggtaatcgc gtgagtacca
     6181 ccggtgccga ttacccctcg cgaggtcttg cggccatggc tcgccgtgct ccagtcgatg
     6241 gattcggcga aaaccgcggc tccttccgca accgccgtca tggggttgac atcggtcgag
     6301 accgcaatgc cgagcgccgc agaaaccttc gcgcgcagcg gcggatactg ggtcgggccg
     6361 ccgacgaaaa cgatccgctc gacgttcgct gggttcaagc ccgaactcgc gaggatttct
     6421 cgagcggcgg caatcgatgc atcgacttgg ctcgcgatga ggcgatccaa cgccgtgcga
     6481 tcgatcgcga tatcaacgaa aatctcttcc ccggccgtgt cgcgggctgc aaggtcggtc
     6541 tcggacaggc tgatggttgc ctgatcgcgc tgcgagagcg cgatcttggc gttctctgcc
     6601 gcccatgtcg ccatgcgaac cagcggtctg tattgcggat tggccgccaa ctcgaacggc
     6661 agcttgaatc gagcgagcaa ccacggcttg acgatctgat cgaacatcgc acgatcgaaa
     6721 tcgcggccgc cgcacatggc gattccccca tgcgcgagca ggctcacgcg gccgccgatg
     6781 ctttcggcga tggcgacatc gagagttccg ccgccgagat catagaccat gaaagccccg
     6841 tcacgaggct cagcgcgcat gacgctcatg acggccgcga ccggttcctg catcagcgcg
     6901 gcgcggccga tgccggccgc ctgcgccgct gtcatggttg cgtccttctg catctggttg
     6961 aaggcggccg ggacgccgat gacgacgccg gtgtcttgat cagcgcgaat cgcagcagga
     7021 aggtagccgt aaaggagttt gagaatttcc gccgaacact cctcgggcgt catcgtcaga
     7081 ttgacggccg caagcagaat cggcgtgctc gtccccatca ggcgcttgaa attgagcgcc
     7141 gcattgtcgg gattgcgggc ggcgttgtta taggcacgca aacccagata ccgattgccg
     7201 cgcttatcga caaagatcgc ggagggcgtg acgtcctgct gctcggggct cttgtaaaga
     7261 tgcacccgct cgccgtcgta gcttgagatc acactgttcg tggtgccaag atcgatgccg
     7321 acgtaattca ccgcggccac tttctcagcg cgacgatgcc ttggcgccgc agcccgtcgc
     7381 ggctcatgac gatcggctcg atcatttgat cgacgagcag cctgtcttcg gcagagaagt
     7441 cacccaagtt gagcgccgtc acggccatac cgggatcgaa ttcgtgtccc tcgagattca
     7501 ccagagtcaa tgccgcggct tgcaagcttt cttcgattgt cttttcaaag taatcgagtt
     7561 gactcgcgta gcgggcggcg tctgcgatgt cgagcttgcg cgccagcctg gcgaaagctc
     7621 gagagaagcg ccagctctcg acagcgagcc cgatgagagc ttgttcgacc tcgatcacaa
     7681 ctcccccctc aattatatcg gcgaccttaa cccatcatgg gtcccctggc catgaattcg
     7741 gggcgcatgc cggggcgcag cgattgttcc aagacgtgaa ccagttctcg attttgcgcc
     7801 ccaaaaaccc cttgtcgggg ctttccttaa cgaatctgga attgctgccg acatttgtct
     7861 agtcagtcga gctaaggtcg ggccgactta gtttaagcca caagatatcg ccctatgcac
     7921 cgtaacccaa cgccctgctt ggaatcccag cgaaccgcgg cattggtccg atgtagataa
     7981 gtccatgaac acgccggcca ctgcagaaca gatcgcgcaa ttacacacga cgccgcggcg
     8041 ccgtgtgttg atcgtggacg atgacgcgat ctttgcgctg ctggcgcaag aaaccctcga
     8101 gcaggccggc tttgacgcca cgatcgccgc caacgcggcc gaggctttcg cggcattcga
     8161 gagggacaaa ccagatttgg tgctgctcga tgtcgacctg ccgggcggca atggttttga
     8221 catatgcgca gcgctacgtg ccttgaactc gggagcggac gtgcccatcg tcatggtcac
     8281 gggtcacgat gacaccaagt cgatcgccca ggcgtacgag gccggtgcca ccgatttcat
     8341 tcacaagccg gtgctttggc cgacgttgcc gcaccgcgtc ggcttcacgt tgcgagcgct
     8401 cgataacatg cgggcgctca agctcagcga acaaaaaaat cgcgcgttgc tgcaggcact
     8461 tcccgatacg ctgttcatcg tcaactcgct gggtgtgctg ttggagcaca taaccggcag
     8521 cgaaagaaaa agcgacgaat ggctggtcgg caaaatgctg gaagaggtgt ttccggcagg
     8581 cctggcccag gcggcgcggc tcagcctgga gaacgccggt agcggagcgc ttacgaccta
     8641 cgagttcggg gtcgagcgag aaagggatcc tcgatggtac gaggcgcggc tgcgtccgca
     8701 agcggacggc aagctgctaa tcgtgattcg cgatcaaacg gaacgtcgca aagccaaggc
     8761 gcgcatcgag tacctggcgt attacgacgt gttgacgcga ttgccgaatc gtcagctgtt
     8821 cgtgcgcgaa gcggcccgcg ccgttgcgca ggctaagcag tccggtcaga tgctggcgct
     8881 gctgtacctc gatctcgatc gcttcaagcg aatcaacgac aacctgggtc acgcggtcgg
     8941 cgatgcattg ctgcaaagtg tggcgcgccg cctggagcaa agcgtcagac agaccgacat
     9001 cgtgtcgcaa gtgggcgagc cgccgaagcc ggaaccgatg cgcgtggcgc gcctcggcgg
     9061 cgatgaattc gtggtgcttc tgaccgacgt catcaatgaa atgcagacgg tcaagatcgc
     9121 cgagcgtatc cagcagtcgc tggctgaacc attcgagtgc agtggccatc gctttgtggt
     9181 gacaccgagc atcggtatcg caatgtttcc gcaggatgcg accgacgtcg acgatctgtt
     9241 ggtcaaggcc gacatggcga tgtatcaggc caaggatcag ggtcgaaacg gccacgcctt
     9301 cttcggccaa tcgatggcgg ttcgatcgct tggccgcctc gagctcgaaa atgacctgcg
     9361 gcgcgctttc gatagcggtg aattccagat ctactaccaa ccgaagctcg agttggcgag
     9421 cggcgcaatc atcggtgccg aggccctgct gcgctggctg cacccgcagc gcggctggat
     9481 cacgccggac tccttcattc cggttgccga agagaccggt ttgatcatcg cgcttggtga
     9541 ttgggtcatc cgcgaggcct gcgttcagct cgcagcgtgg gccaagcagg gcctggggca
     9601 cttgacgctt gcggtcaacg tgtcggttca gcagttcgcc cgggaggatt tcgtcgacac
     9661 ggtgctcggt gcgctcaagg aatttgcagt caaaccgcaa cagctcgaac ttgagatcac
     9721 cgagagcctg ttgatgcgca atgtcgacga tacgaccgcc tgcatgcggc gcttccgcgc
     9781 cgccggtgtg gcgctgtcga tcgatgactt cggtaccggg tattcctcgc tcggatacct
     9841 gcgccagttc ccggtggact ccttgaagat cgaccgctcc ttcgtcaagg atctgcacag
     9901 aagtgaagac gatgcggcaa tctgcgccgc catcatcgcc atggcccgcg aactcaagtt
     9961 gaaagtgatc gcagaaggtg tcgagaacgc cgagcagctc aaatttctgc gccggcaccg
    10021 ctgcgaccag gttcagggat atctgatcag ccggccgatt ccaatggccg aactgcggat
    10081 cttgctgcaa aaggctccgc aggtcgaagg catgtccgtc gattttgctt acagcgcaaa
    10141 aacagcgggg cgctaagatg ccggattcgc gcaagggtgt cattccatga tgcgatggat
    10201 cgacaacttg tcgctcgcat ccaagctgcg cgtaatgatc gtgttggcgg ttgcggtggt
    10261 gctgctgctg gcctccgctc tgtatgcttc ggtcgaggcg ctggctctgc gcgcaaacct
    10321 cgcccagcag ctgctcatcc tgacgacggc agcaggtcaa aatgcggccg ctccactcgc
    10381 gctccacaat cgcaatttgg cgcgcaatgc gctcgaaccc ttgcatgcgg acccaaccat
    10441 gcgtgcggtt gcgctgtacg atgctgccgg ccacatcttt ttgaacttct ctgcgccggg
    10501 cacgaagctg ctcagcgacg acaggctcaa ggataatcag gcgctgcatt tcgacggcct
    10561 gacgcgcacc gacatccaag tccacgtgat cttggacggc aagccggtgg gtacgctgcg
    10621 tctgcaggcg gacctgagcg cgcagttcta cccgcagttc aagagttccg tgcaattcat
    10681 gttggtcgga tttgcgctcg cgggaaccgt tgccttcctg ctgtcgagcc ggttgcggcg
    10741 ggtcatttcg atgccggtcg gaaatctggt gcaggttacc cgggacgtcc tcaagagcaa
    10801 ggacttctcg atcagagcgg acaagatcag cagcggtgag ttcgggactc tgatcgacgg
    10861 tttcaatgaa atgctcgccg agctcgagag acgggatcga aatctgcgcg cctatcagaa
    10921 cgagttggaa agactggttc gcgagcgcac ggtgcagctc gatatagagg tcgccgaagc
    10981 tcaggaagcc gcgaaacgcg ccgaaggcgc gagccgtgcg aagagcgatt tcttggcgcg
    11041 catgagccac gaaatccgca ccccgatgaa cggcgtgctc ggcatggcgg aactgttgcg
    11101 ccactccacc acgctcgacg agcgccagcg ccgctacgcc atcacgatcc atgaatccgg
    11161 cagcgcgctg ctgcagatca tcaacgacat cctggatttc tcgaaaatcg aggccggcaa
    11221 gctgcaaatc gacattgcgc cgttctgcgt gcgcgaggtc gtcgaggacg cggtggaaat
    11281 tctggccgaa cgggcgcacg ccaaggggct cgaactgatc tgcgatatac cgacgcgaat
    11341 cgcaaccaag gcgagcggcg atgcgctgcg tctgcgccag gtactcatca atttgatcag
    11401 caatgccgtt aaattcaccg agcatggcga agtctgcatc cgggtcaaca gtgtggacgc
    11461 agggcttcac aactcgtcgt tcgacttcga ggtctcggat accggcatcg gcatcaaacc
    11521 cgagaattgc gccaccattt tcgaatcgtt cgcccaggcg gacgtctcga cgacgcgcca
    11581 gtacggcggc acggg
//
LOCUS       DASWZT010000475         9342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_42720, whole genome shotgun sequence.
ACCESSION   DASWZT010000475 DASWZT010000000
VERSION     DASWZT010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9342
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42720"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..2032)
                     /locus_tag="VGE92_13630"
     CDS             complement(20..2032)
                     /locus_tag="VGE92_13630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1314917.1"
                     /translation="MTRLFGRGDGPLLHGMLAGFVIMLISSLGLFYLSHSAREAQLDA
                     VRTELKQLARVAATLVDGDRHELLKSQAQAGGADHLALLAPLVKFHKATSDIIYVYTA
                     ILDEQRIYYVLGTDYLYRVDGDNESPELIMAPHDTWDPTLRRALTEHVVAVNEEPVKE
                     AVRSYMSAYAPFYDSQGRFVGVIGVDMWVRDFDMRVAAIRRAGISAFAAVAMLSLLAG
                     LVVFRLSRTASRARRRDRLVQARLAEAKQQAEVQAQRAEAASKAKSDFLAMMSHEIRT
                     PMNGVLGFVHLLLDTPLTVEQREFAQMVQRSGDALLIIINDVLDYSKIEAGRMSVEQI
                     DVDLRSVCNEVRKILQSAVSARELDISLCYDAALPGMIKGDPVRIRQVLLNLAGNAVK
                     FTERGAVRIEVSRCDAAQVKISVVDSGIGITEEQMQRMFSRFTQADSSTTRRYGGTGL
                     GLAISKTLVELMGGSIGVQSIAGQGSTFWFTLPLGAAATAPQAMAPPAIPVLTAVVEP
                     PAQADTPSVIATPPEAPPPAPAVAAEPSAARLLLVEDNFVNQRVAVYMLNKLGFQVEV
                     ANNGREALAKLSKTNYELVLMDCQMPEMDGFEATRAIRDRSSAVLDHDVPVVAMTANA
                     FPEDRSRALAAGMNDFLAKPVDRAVLAATLGKWLEPPAETQALAAG"
     gene            2206..3210
                     /locus_tag="VGE92_13635"
     CDS             2206..3210
                     /locus_tag="VGE92_13635"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY1314918.1"
                     /translation="MLKRILVILLAVVVVLAVVGLLLPRNIRVQRSVSIARPASLVYA
                     TVNSFQLFPRWSPWQNLDPNMHQTTQGPRDGVGARLVWSGNDKVGSGTQLITASTANR
                     SVASDLDFGKMGVAKSNLLLDPQADGTRVTWALDIDMGASPVGHYFGLMMDGMIGKDF
                     ARGLANLKTLVEGMPNVDIADFVAEPVQMSAVPLIVVSETAAPDSASISKAYADGYAQ
                     IGKFMAKNKLHQNGAPLGIDGEMSATTFAFQAGMPVDRADVVAADNVRVIQSYSGKAL
                     KTTHVGPYDTLAKTYAEFRAYLAAHDYSPAGGTISWYVDDPGSTPAEKLRTEIYWPIR
                     "
     gene            3297..3899
                     /gene="wrbA"
                     /locus_tag="VGE92_13640"
     CDS             3297..3899
                     /gene="wrbA"
                     /locus_tag="VGE92_13640"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF002999.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEY1314919.1"
                     /translation="MAKVLVLYYSSYGHVETMANAIAEGARSGGASVDVKRVPETVPA
                     DAAKAAHFKLEQRARVATIDELANYDAIVVGSPTRFGRIASQMASFLDQAGGLWMRGA
                     LHGKVGGAFTSTATQHGGQEVTLFSIITNLLHFGMVIVGLPYSHQGQMTLDEIVGGSP
                     YGATTVAGSSGQRAPSALDLAGAHHQGKLIAETANKLFPG"
     gene            complement(3916..5406)
                     /locus_tag="VGE92_13645"
     CDS             complement(3916..5406)
                     /locus_tag="VGE92_13645"
                     /inference="COORDINATES: protein motif:HMM:NF023689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3612 domain-containing protein"
                     /protein_id="HEY1314920.1"
                     /translation="MAGLIRQGHFLGTKLRSLRKRNGLTLDELSARCVQIDAAGAPSV
                     SYLSMVETGKRMPSAVMLDMLATIFSKDARWFLDENTELDTPPAPRERGGLEAMPLEP
                     AFLFSHELLQNALPELLSQTGTTGRQFAQLLIRVWQETRHNNFPDIERAAEECGERQM
                     PLDLDAVLAICKRLSLEVEWFDGDARKPSGALVRSQFEAPGTVLINKRLRNQEERLKY
                     ELAFFIGHKILHNGDGAISPHSAIHAGDGESSGSAAGMGAQDVLYAWRDFECSFFAGA
                     LLCPRVPFRRFLIREAHSVGSCRKLGVTPALVMRRMTAVSPYRHWHFFDAYAPGYLRA
                     VYRGNGIPLPWGNMSLVSDPCPRWAVFRLLQMTPPAPPMQRPKSQISVMQDGKRARLY
                     CCHSLLTRDAMDEVHVLSVGVDLLPALDAQGYDGEEMVETIAQACRLGGGDGVIPERA
                     AAGIRAASQVLNIAWIAEALDSPASVICPRSGACPRQPQRCAGGLK"
     gene            5535..6815
                     /gene="aceA"
                     /locus_tag="VGE92_13650"
     CDS             5535..6815
                     /gene="aceA"
                     /locus_tag="VGE92_13650"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002554010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEY1314921.1"
                     /translation="MTDKVTDRWLGIERPYSTADVARLRGSVHIEYSLARIGAEKFWR
                     QLHSESVIAGLGCMTGNQAIQTVQAGLKAIYCSGWQVAGDANTAGEVYPDQSLYPVDS
                     VPKMVERINNALLRTDQIHHMDGKSDIDWIVPIIADAEAGFGGNLNAFELTKALIRAG
                     AAAVHFEDQLSSAKKCGHLGGKVLVSTSEAINKLVAARLAADVLGVPTVIIARTDADA
                     ADLLLTDHDPRDARFLTGQRSTEGFFYVKAGIEQAIDRGLSYAPYADLIWCETSKPDM
                     AEARRFAAGIHAKFPGKMLAYNCSPSFNWQAKLDAASMKRWREDLAAMGYRFQFITLA
                     GWHALNLSMFELASAYRTDGMYGYSQLQQREFSQEAEGYRAAKHQAFVGTGYFDAVQT
                     AISSGAHSTGAMAGSTETEQFTSGQKSGPKRVVA"
     gene            6826..8562
                     /gene="aceK"
                     /locus_tag="VGE92_13655"
     CDS             6826..8562
                     /gene="aceK"
                     /locus_tag="VGE92_13655"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF002804.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HEY1314922.1"
                     /translation="MSAAVTSATKKDGVTAKDHAALIAEHFRLYNEEFGRITRRAALN
                     FLAEDWQTAQQDAVARIELYEQRVARSVAALAAQLQAKCNDVALWIDIKRAYELIVAR
                     RPDSDFYRTFFNSVTRDLFGTVGVNPEVEFCATNVGRASGAVPIRVYRVGGSLPTAVR
                     EILTDLPFGAAIEDTDAAVHRISAEIGRYFQTGRLSGQPESIELIEPLFYRGMQAFVV
                     GRLIGDGSITPLVLAFTNSPKGVHIDTVLLSRADVGSLFGYARSYFHVDLPVVSAAIT
                     LLRSFMPRKSIDELYTVLGRAKQGKTERYFALQRHLEKSIDSFVHAPGERGLVMIVFT
                     LPSHDLVFKVIRDRFGAPKTSTREDVIERYQFVFRHDRAGRLVDAQEFKRLRLPRARF
                     MPTLVEELLNEASVSCRIEGDDLIVEHCYIERRLRPLNLFLREAEPAAAESAIIDYGD
                     ALRDLAVSNVFPGDLLLKNFGVTSHGRVIFYDYDEICLLTDCVFRDLPQASCEEEETS
                     AEPWFHCGPRDVFPEQWLPFLSIPPALCSVFKQHHSDLLTAAWWRDQASSHQPAAAAA
                     CTSPASPGAPAA"
     gene            complement(8576..9268)
                     /locus_tag="VGE92_13660"
                     /pseudo
     CDS             complement(8576..9268)
                     /locus_tag="VGE92_13660"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
BASE COUNT         1769 a         3001 c         2884 g         1688 t
ORIGIN      
        1 tctcaattcc gcttgagggt cagccggcgg cgagcgcctg agtctcggcc ggcggttcga
       61 gccacttgcc gagcgtggct gcgagcaccg cgcgatcgac cggcttggcg agaaaatcgt
      121 tcatgccggc ggctagcgcg cgtgaacgat cttccggaaa cgcgttcgcc gtcatggcga
      181 ccaccgggac atcgtgatcg agcaccgcgg agctgcgatc gcgaatcgcg cgcgtcgctt
      241 cgaatccatc catttccggc atttggcagt ccatcagaac gagctcgtaa ttcgtcttgc
      301 tgagcttggc gagggcctcg cgcccgttat tcgccacctc gacctgaaac cccagtttgt
      361 tgagcatgta caccgcgact cgttgattga cgaaattatc ctcgaccagc aacaggcgcg
      421 cggcgctggg ctccgccgca accgccggcg caggcggcgg cgcctcgggc ggcgtggcaa
      481 tcacgctcgg cgtgtccgcc tgggccggtg gctcgaccac ggccgtcaac accgggatcg
      541 ccgggggcgc catcgcctgc ggtgccgtcg ccgccgcgcc gagcggcaag gtgaaccaga
      601 atgtcgagcc ctgaccggca atactctgca ccccgatact gccgcccatg agctcgacca
      661 gtgtcttgct gatggcaaga cccagcccgg taccgccgta acgccgcgtg gtcgacgagt
      721 cagcctgcgt gaagcgcgag aacatccgct gcatctgctc ctcggtgatg ccgatgccgc
      781 tgtcgacgac gctgattttt acctgcgcgg cgtcgcaccg cgagacctcg atacggactg
      841 cgccgcgctc ggtgaacttg accgcgttgc ccgccaaatt caacagcact tgccgaatgc
      901 gcaccggatc tcccttgatc attccgggaa gcgcggcgtc atagcacagg ctgatgtcga
      961 gttcgcgcgc gctcaccgcg gattgcagga tcttgcgcac ctcgttgcac accgaacgta
     1021 gatcgacgtc gatttgctcg acgctcatcc ggcccgcctc gatcttcgaa taatcgagca
     1081 cgtcgttgat gatgatgagc agcgcatctc ccgagcgctg caccatttgg gcgaattcgc
     1141 gttgctccac agtcaatggg gtgtcgagca gcagatgcac aaagccgagc acgccattca
     1201 tgggggtgcg tatctcatgg ctcatcattg cgagaaagtc gctcttggcc ttcgatgccg
     1261 cctcggcgcg ttgtgcctga acctcggcct gttgttttgc ctccgccagc cgcgcctgca
     1321 ccaggcgatc ccggcgccgt gcgcgcgagg cggtgcggct cagccggaag acgaccaagc
     1381 cggcgagcag cgacagcatg gcgacggcgg caaacgcgct gatcccggcg cggcggattg
     1441 ccgcgacgcg catgtcgaaa tcgcgcaccc acatatcgac gccgatcacg ccgacgaatc
     1501 gtccttgcga gtcgtagaac ggcgcgtagg cgctcatgta gctgcgcacc gcttccttga
     1561 ccggctcctc gttgactgcg accacatgct ccgtgagcgc acggcgcagt gtcggatccc
     1621 aggtgtcgtg cggcgccatg atgagttcag gcgattcgtt gtcgccgtcg acgcgatata
     1681 ggtagtccgt tcccaggacg tagtagatgc gctgctcgtc gagaatggcg gtatagacat
     1741 agatgatgtc ggaggtcgcc ttgtgaaatt tgaccagcgg cgccaatagc gccagatgat
     1801 ccgcgccgcc ggcctgcgcc tgcgacttca agagctcgtg acggtcacca tcgaccaagg
     1861 tcgcggcaac gcgagccagc tgcttgagtt cggttcttac ggcgtcgagt tgggcctcgc
     1921 gcgccgaatg cgagaggtag aacaggccga gcgaggaaat gagcatgatg acgaatcccg
     1981 ccaacattcc gtgcagcagc ggaccgtcgc ctcggccaaa caggcgtgtc aaaatgaaca
     2041 ttcccaaata cccaatatcg gctcttcatc tcgaagctgt agcgtacggg tttccggggt
     2101 tgccggccgc gagcaagccg gagaactgcg acggcgttca atattggcct tgcaaatcgc
     2161 ggctcgcgaa gggttcaatc cgctcaggga cggcgggagg ggacgatgct caagcgaata
     2221 ctggtaattc tgctcgcggt cgtggtggta ttggcagtgg tcgggctgct gctgccgcgc
     2281 aacattcgcg tgcagcgctc ggtcagcatt gcgcggcccg cgagcctcgt ctatgccacg
     2341 gtcaattcat ttcagctgtt tccgcgatgg tcgccgtggc aaaacctcga tccgaacatg
     2401 caccaaacca cgcaaggtcc gcgcgacggc gtcggcgcga gactcgtgtg gtccggcaat
     2461 gacaaggtcg gttcaggcac gcaactgatt accgcctcga ccgccaatcg ctcggtcgcg
     2521 agcgatcttg atttcggaaa aatgggagtc gcgaagtcga atttgctgct cgatcctcag
     2581 gcggacggca cgcgcgtcac gtgggcgctc gacatcgaca tgggtgcaag ccccgtcggc
     2641 cattactttg ggctcatgat ggatggcatg atcggcaagg acttcgcgcg cggcttggcg
     2701 aatttgaaga cgctggtcga aggcatgccg aacgtcgaca tcgccgactt cgttgccgag
     2761 cccgtgcaaa tgagcgccgt gccgctgatt gtcgtcagcg agaccgccgc gcccgattcg
     2821 gcgtcgattt ccaaggcgta cgccgacggc tacgcgcaaa tcggcaagtt catggccaag
     2881 aacaagctgc atcaaaacgg cgcaccgctc ggcatcgacg gcgagatgag cgcgaccacg
     2941 tttgccttcc aagcgggcat gccggtcgac cgtgccgatg tcgttgccgc cgacaatgta
     3001 cgggtgattc aaagttattc gggcaaggca ctgaaaacca cccacgtcgg cccgtacgac
     3061 accttggcga agacctacgc cgagttccgc gcttacctgg ccgcgcatga ttacagtccc
     3121 gccggcggga ccatttcttg gtacgtcgat gaccccggtt ctacgcctgc ggagaaattg
     3181 cgcaccgaaa tctactggcc catccgctag tgcgcgtcaa gcgaatgcca ttgccccatc
     3241 ttgtcagcga atccgcgctg acataacctt gccgcaaaca gcaacccggg aattcgatgg
     3301 caaaggtatt ggttctttat tattcctcct acggtcacgt cgagacgatg gcgaacgcca
     3361 ttgccgaggg tgcccgctcg ggcggcgcga gcgtcgacgt caagcgcgtg cccgaaaccg
     3421 tgcccgcgga tgcggccaag gcggcacact tcaagctgga gcagcgtgcg cgggttgcca
     3481 ccatcgatga gctcgcgaac tacgatgcca tcgtcgtcgg cagcccaacc cgcttcggac
     3541 gcatcgcctc gcagatggca agtttcctcg atcaagccgg cggcttgtgg atgcgcggcg
     3601 cgttgcacgg caaggtgggc ggggcgttta cctcgaccgc gactcagcac ggcggtcagg
     3661 aggtcacgct gttttcgatc atcacgaatc tattgcattt cgggatggtg atcgtcggcc
     3721 tgccgtacag ccatcaaggc cagatgacgc tcgacgagat cgtcggcggc agcccctacg
     3781 gcgcgaccac cgttgccggc agttcgggac agcgcgcgcc cagcgcgctc gacctcgcgg
     3841 gcgcgcacca tcagggcaaa ttgatcgcag aaaccgccaa caaacttttc cccggatagg
     3901 ccccggggcg gggaattact tcaggccgcc ggcgcagcgc tgcggctggc gcgggcaggc
     3961 gccgctgcgc gggcagatga cgctcgcggg cgagtccaac gcctcggcaa tccaagcgat
     4021 attgagaacc tgcgacgcgg cgcggatccc ggccgcggcc cgctccggaa tgaccccatc
     4081 gccgccaccg agccggcatg cctgcgcaat cgtctcgacc atttcttcgc cgtcgtagcc
     4141 ctgggcatcg agcgccggca acagatcgac acccacggac agcacgtgca cttcgtccat
     4201 cgcatcgcgc gtcagcaggg aatggcagca gtacaaccgc gcccgcttgc catcctgcat
     4261 caccgagatt tgtgacttcg gcctctgcat gggaggcgcg ggcggcgtca tttgcagcaa
     4321 acgaaatacc gcccagcgcg ggcacgggtc ggacacgagg ctcatattgc cccacggcaa
     4381 cggtatgcca ttgccgcgat ataccgcgcg caagtatccc ggcgcgtacg catcgaagaa
     4441 atgccaatgc cggtaagggg acacggccgt catgcggcgc atgaccagcg ccggcgtgac
     4501 accgagtttg cggcagctgc cgacgctgtg ggcttcgcgg atcaaaaagc gccgaaacgg
     4561 tacccgcggg cacagcaggg cgccggcaaa aaaactgcac tcgaagtccc gccacgcata
     4621 cagcacgtcc tgcgcgccca tgccggccgc cgagccggac gattcgccgt caccggcgtg
     4681 aatggcgctg tgcggagaaa tcgcgccgtc gccattgtgc aggatcttgt gcccgatgaa
     4741 gaacgccagt tcgtacttca ggcgttcttc ctggttgcgc aatcgcttgt tgatcaacac
     4801 cgtcccgggc gcttcgaatt gcgagcgcac caacgcgccg ctcggcttgc gcgcatcgcc
     4861 gtcgaaccac tccacttcga gcgacaggcg cttgcaaatg gcaagcaccg cgtcgaggtc
     4921 gagcggcatt tggcgctcgc cgcactcctc ggccgcccgc tcgatgtccg gaaaattgtt
     4981 gtggcgggtc tcttgccaga cgcgaatcag caattgcgcg aattgccggc cggtggtccc
     5041 ggtttgcgag agcagctccg gcagtgcgtt ctgcagaagc tcgtgcgaaa acagaaacgc
     5101 cggctcgagc ggcatggcct caagcccgcc ccgctcgcgc ggcgcgggcg gggtatcgag
     5161 ctcggtgttc tcatcgagaa accagcgtgc atccttgctg aagatggtgg ccagcatgtc
     5221 caacatgacg gccgagggca tgcgcttacc ggtttccacc atgctcaagt aggacaccga
     5281 tggggcgccc gcagcgtcga tttgcacaca ccgggccgat aactcgtcca aagtcaggcc
     5341 gttgcgcttg cgcaaggacc gcagtttggt acccaaaaag tgcccctgcc ggatgagtcc
     5401 cgccataacc acaccttccg gttgtgaaat tcacactgtc aaatttctaa tgtgtaaatc
     5461 cgcgcttccc atactttaga ctgcctctat ctcgagaagc aagctcgcag ccttttgcac
     5521 ctaggagaat tgaattgact gacaaagtga ccgaccgttg gctcggaatc gaacgcccct
     5581 acagcacggc cgacgtcgcg cggctgcgcg gctccgtgca catcgaatat tcgctggcac
     5641 gcatcggcgc cgagaagttc tggcgccaac tgcacagcga gtccgtgatc gcgggcttgg
     5701 gctgcatgac cggcaatcag gccattcaaa cggtgcaagc cggcctgaag gcgatctact
     5761 gctcgggatg gcaagtagcc ggagatgcca acaccgcggg cgaggtgtac ccggaccaga
     5821 gtctgtaccc cgttgattcg gtgccgaaga tggtcgagcg catcaacaac gcgctgctgc
     5881 gcaccgatca gattcaccac atggacggta aatccgacat cgattggatc gtaccgatca
     5941 tcgccgatgc cgaagccggc ttcggcggca atctcaatgc cttcgaattg accaaggcgc
     6001 tgattcgagc gggcgcggcc gccgtgcact tcgaggatca attgtcgtcc gccaagaaat
     6061 gcggtcacct cggcggcaag gtcctggtat cgaccagcga agcgatcaac aagctcgtgg
     6121 ctgcacgtct tgcagccgat gtactcggcg tacccaccgt catcattgca cgcaccgacg
     6181 ccgatgccgc ggatttgctg ctcaccgacc atgatccgcg cgatgcgcgt ttcctcaccg
     6241 gacaacgctc gaccgagggc ttcttctatg tcaaggccgg gatcgaacag gcgatcgatc
     6301 gcggcttgtc ctatgcgccg tacgccgatc tgatctggtg cgagacttcc aagcccgaca
     6361 tggcggaagc gcgccgcttt gccgccggca ttcacgccaa gttccctggc aagatgctgg
     6421 cctacaactg ttcaccgtca ttcaactggc aggccaagct cgatgcggcc tcgatgaaac
     6481 gctggcgcga agatctggcg gcgatgggct atcgtttcca gttcatcacg ctggccggct
     6541 ggcacgcgct gaatctgtcg atgttcgagc tggcctccgc gtaccgcacc gacggcatgt
     6601 acggctactc gcagctgcaa cagcgcgaat tcagccagga agccgaaggc taccgcgccg
     6661 ccaagcacca ggcgttcgtc ggcacggggt atttcgatgc cgtgcaaacc gcgatcagct
     6721 cgggcgcgca ttccaccggc gccatggccg gcagcaccga gaccgagcaa ttcacctcag
     6781 gccagaaatc gggcccgaag cgcgtggtcg cgtaaggccc cgcgcatgag tgccgccgtc
     6841 acttccgcta cgaagaagga cggcgtcact gcgaaagatc acgcggcgct gatcgccgaa
     6901 cactttcgcc tctacaacga ggaattcggg cgcattacgc gccgcgctgc gctcaacttc
     6961 ctcgccgagg attggcagac ggcgcagcag gatgcggtcg cgcgcatcga gctgtacgag
     7021 cagcgcgtgg cacgcagcgt cgcggcgctc gccgctcaat tgcaggccaa atgcaacgac
     7081 gtggctctgt ggatcgacat caagcgtgcc tacgagttga ttgtcgcgcg gcgccccgac
     7141 agcgatttct accgcacgtt cttcaactcg gtgacccgcg acctgttcgg aacggtcggc
     7201 gtcaatccgg aagtggaatt ctgcgcgacc aacgtcgggc gcgcgtccgg cgcggtgccg
     7261 atccgcgtct accgagtcgg cggctcgttg cccacggcgg tgcgcgagat cctgaccgat
     7321 ctgcccttcg gcgccgccat cgaggacacc gacgcggcgg tgcatcggat cagcgccgag
     7381 atcggccgct attttcagac tggccgattg agcggtcaac ccgaatccat cgagctgatc
     7441 gaacccttgt tctatcgcgg catgcaggca tttgtcgtgg gacgcctgat cggcgacggt
     7501 tcgatcactc ccctggtgtt ggccttcacc aactcgccca agggcgtgca catcgacacg
     7561 gtgctgctgt cacgcgccga tgtcggctcg ctgttcggct acgcccgttc gtattttcat
     7621 gtggatctgc cggtggtgag cgccgcgatc acgctgctgc gctcattcat gccgcgcaaa
     7681 tccatcgacg agttgtacac ggttttaggc cgggccaagc aaggcaagac cgagcgctat
     7741 ttcgcgctgc agaggcacct cgagaaatcc atcgacagct tcgtgcacgc gccgggggag
     7801 cgcggcctcg tgatgatcgt cttcaccttg ccgtcgcacg atctggtgtt caaggtgatc
     7861 cgtgatcgtt tcggtgcgcc gaaaaccagc acccgcgagg acgtcatcga acgctaccag
     7921 ttcgtgtttc ggcatgaccg cgccggccgg ctggtcgacg cgcaggaatt caagcgtttg
     7981 cggctgccgc gcgcgcgctt catgcccacc ttggtggagg agctgttgaa cgaggcgagc
     8041 gtcagctgcc gtatcgaagg cgatgatttg atcgtcgaac actgctacat cgagcggcgt
     8101 ctgaggccgc tcaatttatt cttgcgcgaa gccgaaccgg ccgcggccga gtcggccatc
     8161 atcgattacg gcgacgcgct gcgcgatctg gccgtgagca atgtgttccc gggcgatctg
     8221 ttgctcaaga attttggcgt caccagccac gggcgcgtga ttttctacga ctacgacgag
     8281 atctgcctgc tgaccgactg tgtgtttcgc gatttgccgc aggcgagctg cgaggaggag
     8341 gaaaccagtg cggaaccctg gttccactgc ggaccgcgcg acgtcttccc ggagcaatgg
     8401 ctgcccttcc tcagcatccc gccggccctc tgcagcgtgt tcaagcagca ccactcggat
     8461 ctgctgacgg cagcatggtg gcgcgatcag gcgtcgtcgc accaaccggc ggcggcagca
     8521 gcttgtacat caccggcgtc accaggcgcg ccagcagcgt agaggaaatc aggccgccga
     8581 tgatcacgat ggcgagcggc gaatacaggc ccgatccctg cagagcgagc ggcaacaagc
     8641 cgccgatggc agtcgccgag gtcagcaaga tcggcaggaa gcggatttcg ccggcctcct
     8701 ggattgcctg atcgagcggc atgccttggg cgcgcagccg gttggtgaaa tccaccagca
     8761 gaatcgaatt ttttatttct atgccgacca gcgcgatcat gccgatgatc gaggtgaacg
     8821 acagcgtgta tccggtcgca agcagcgcca cgagcgcacc cgtaaccccc aaaggaatca
     8881 ccccggcaac gatcagagtg gaacggaagc tgccgaattc gagcaccagc accgccagga
     8941 tgccgaaaac cgccaccaga atggccggca acaggccgcc gaagctttcc tgctgcgctt
     9001 cgacttcgcc cgcgacaacg aagcgataac ccggcggtaa gccgatcgcg gcgagttgcc
     9061 tgaccacggc ggcggtgacc cgcgccgtgt tgaatcccgt ctgcgtcgac gcggacaccg
     9121 tcacttggcg ctcgcggttg tagcgcgtaa tcgagttggt gacggtttcg aatcgcggat
     9181 tcgatatttg gcgcagcggc acctgacgcc cgccggcggt gctcacttcg atcgagtcga
     9241 gcgcctcgat gctcggccgc tcctgcatcg gcaaacgcag cgtgatgtcg tattcatcgc
     9301 cgttattggc gcgaaattgc ccggcggcga gcccggcgac cg
//
LOCUS       DASWZT010000476         8734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_66615, whole genome shotgun sequence.
ACCESSION   DASWZT010000476 DASWZT010000000
VERSION     DASWZT010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8734
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66615"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VGE92_13665"
     CDS             <1..256
                     /locus_tag="VGE92_13665"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY1314923.1"
                     /translation="SALRQLQQESLSEAAAVTATGKALQQAQYRYKAGLVTYLEVATT
                     ENTALQAQLTNVNIQLRRMNASVLLVKALGGGWQNETPRQ"
     gene            complement(287..913)
                     /locus_tag="VGE92_13670"
     CDS             complement(287..913)
                     /locus_tag="VGE92_13670"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009394731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEY1314924.1"
                     /translation="MNAASIFSSVPIALPALPYEQSALQPVISANTLGFHYGKHHKTY
                     VDTLNKLIAGTEYADMPLEKIVRATAGQAQHAEIFNNAAQAWNHTFYWHSLRPQGGGE
                     PPAALKGLMDASFGGVDVCKKELSKAAVGQFGSGWAWLVQDGAKLKIVKTCNAHTPLS
                     EGLKPLLTIDVWEHAYYLDYQNRRVDYVNALLDKLANWEFAAGNLGRS"
     gene            complement(1023..2024)
                     /locus_tag="VGE92_13675"
     CDS             complement(1023..2024)
                     /locus_tag="VGE92_13675"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEY1314925.1"
                     /translation="MSKTHNKTCYAGWTVFGAAALIVPWSLVHAGRTIEEHRAADPQG
                     EVEIINVSGSVEVDGWDRSEVDVSGTAGDNVERVDVTSAGRRTSIRVVSRAIHSWSSD
                     GEARLVIHIPARSAVTATLVSADFKVSGVLGDQKLQAVSGNLSGDVGGDLRASTVSGD
                     VRMTARAAKRIDVKTISGNIHLTGGGGEVDITTVSGTESVELGAVTGGRFKAISGNIT
                     AALGLAADARIEAEMVSGDLNFKFSAMPSAEFDVQTISGDIKNCFGPKPIESRYGPGS
                     RLQFTNGDGRGRVRIDTKSGDVRLCAAGAAATHVSSRVAPFAVVRARPARMVLPYVL"
     gene            complement(2028..2549)
                     /locus_tag="VGE92_13680"
     CDS             complement(2028..2549)
                     /locus_tag="VGE92_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314926.1"
                     /translation="MNDPMRPSLDEQLAHMPRAIAPPRNLWPGIARRLPRRPNRPRSV
                     AFAVAAGAAGACIASALTWAVLRSHPGSPAVPLAAQVRSFDEPVNPRYVAARDSLELN
                     FRERLKLLDPATRARIEASLAVIRQAHEDIRKALASDPASPVLEQLWQSTWRDELDLY
                     DNVVQATQPMTRT"
     gene            complement(2546..3097)
                     /locus_tag="VGE92_13685"
     CDS             complement(2546..3097)
                     /locus_tag="VGE92_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008439617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY1314927.1"
                     /translation="MLQAAEASDAAEPQGLIAAAVAGDLAAFESLYRQFAPRVYGLCL
                     RLTGQREAAEDCTQESFVAAWRALAGFEHRSRFSTWLHRIAVHTVLARRRGLRLEYEV
                     AAPAAGLPEVADPAGDGAPLDLERAIARLPAGARDVLVLVGIYGFSHMEAGRMLGIAE
                     GTCKAQLHRARGLLAAALGLEHS"
     gene            complement(3188..3601)
                     /locus_tag="VGE92_13690"
     CDS             complement(3188..3601)
                     /locus_tag="VGE92_13690"
                     /inference="COORDINATES: protein motif:HMM:NF039479.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6524 family protein"
                     /protein_id="HEY1314928.1"
                     /translation="MRNLSIGGLMGRFLASLILVAATFNPSGYSFIQWIAGNFPHLQP
                     LQLVVGIALLAAWLFFLHATWRSLGTFGVALGVAFFVAMAWLFSSWGWFSLSNHNSVI
                     WVAVFMLACLLTLGLCWALIQARVSGQAVVEEVKR"
     gene            complement(3859..4134)
                     /locus_tag="VGE92_13695"
     CDS             complement(3859..4134)
                     /locus_tag="VGE92_13695"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY1314929.1"
                     /translation="MKATGTLQVHNERVIVTEWRFAPGADTGHHVHAHDYVVVPLTSG
                     TLRLVDAQGTRDASLQAGVSYARPAGVAHNVINANDYEFRFVEIELK"
     gene            4262..5500
                     /locus_tag="VGE92_13700"
     CDS             4262..5500
                     /locus_tag="VGE92_13700"
                     /inference="COORDINATES: protein motif:HMM:NF004788.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate carboxypeptidase"
                     /protein_id="HEY1314930.1"
                     /translation="MMRVVAASAAALTWLCGAGAQASPQLNDSVYRAVLANRAAALEL
                     LEQIVDIDSGTGDVAGGTQVQAVIAGRLKALGAQVSTAPAEAPGLPDNLLAVFHGTGK
                     GKLLIIAHIDTVFAPGTAAHRPFKMDQQRAFGPGVGDEKAGVVNALTALTILHDIGFK
                     NYATITLLLDDSEERGSPGSRQLITSLVKAHQVEFNMEPGDPPDALTVWRKGSASIRV
                     LVNGRAAHAGMAPQDGRNAAVELVHQLAGLEGAFPHSGAGTTVNLTILKSGERNNIIP
                     DLAEATLNVRYRKPEDLDAVLARVESGARTTLVEDTRVTVTHDPAYPPLTENAQIDAL
                     AARAQAIYAELGKSIALSGNGGASESALAMAEGTPALDGLGFVGGDFHTDHEWIDLGS
                     VVPRLYLFTRLLMETGAKPP"
     gene            5595..6128
                     /locus_tag="VGE92_13705"
     CDS             5595..6128
                     /locus_tag="VGE92_13705"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEY1314931.1"
                     /translation="MQCARCRAPLQRPANAAIDTGISLAATALVLLVLSNLYPLVAMH
                     VNGSSRETTLIGAARGLHAQGFDSLAVLVLMTTVIAPLLQIVTLLYVLLPLRRGRQAR
                     GQHAVLRVLTQIRPWSFMEVFMLGALVALVRLSKFADIVPGISLWTSVLFMLSLSALT
                     SITSPEQLWRWVEGSRR"
     gene            6125..6745
                     /locus_tag="VGE92_13710"
     CDS             6125..6745
                     /locus_tag="VGE92_13710"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEY1314932.1"
                     /translation="MSSPATGRSLGLLVCLSCRATVRRVQSVYSRCPRCHARLHERKP
                     HSLAITTALVIAAAVLYVPANVLPVMSTRTLIDDESDTIMSGVLVLLHSGSWPIAILV
                     FIASIVVPILKILALGVLLYSAWRRSTRRRLQRSELFRMVEFIGRWSMLDIYAVSLLA
                     TLVQIQSFASIIVGWGAWSFGAVVILTLLAARTFDERLLWDTPPHD"
     gene            6738..8384
                     /locus_tag="VGE92_13715"
     CDS             6738..8384
                     /locus_tag="VGE92_13715"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY1314933.1"
                     /translation="MTEPANPDLEELPSAVPAQSRWSRLPLIWILPTVVVLAGAFVVV
                     HEKLAQGTAIDIQFRNAEDLEANKTKIRYKDVEIGEVRDIHVSEDRNSVIVNALIHRD
                     ATDYLVSDTRFWVVRPRVSAAGVTGLGTLVSGANISVDVGKSTARRRYFVGLEVPPIV
                     TANLQGREFVLHADDLGSLSVGSSVFYRHITAGQVVAYNLDPSGTSVTLKVFINSPFD
                     TYVTAATRFWQASGIDMSLNSDGVKLRTESLTAILQGGVAFQALPGGATTPVPNDSSF
                     TLYQDEDRAMRPVETEVRSFVMYFKGSLRGLSAGAPVELHGIGVGEVRSVDVEYDRDA
                     GSLSFPVAVDIYPQRIRGRKASSARSAKAQDDPETSSRRLVDSLVAHGLRAELKTGNL
                     LTGQKYVDLDMHLDVPAEKVWWNEQPSVFPTVSNGLDEIQDSLGSIARRLGKVPFDQI
                     SYRLLSTLTSLEQTLKSTDQLMRHVDGTLAPQVEATLVEAQAAMKNAKEILSRDAPLQ
                     SDLGATLLQLSRAAKAVSALVDYLERHPESLLRGKPGDSP"
     gene            8381..>8734
                     /locus_tag="VGE92_13720"
     CDS             8381..>8734
                     /locus_tag="VGE92_13720"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEY1314934.1"
                     /translation="MKLPLSALWLLSMLCACAASRPDRFYVLSVQPPGAVDARTMPAT
                     EVSLKVALPSMIDRGEMIIQTSADGVQVLEHERWAAPLTDMVTETLARDLERRRADLL
                     VAGAGAVLAAGALVKI"
BASE COUNT         1646 a         2775 c         2662 g         1651 t
ORIGIN      
        1 gagcgcgctt cggcaactgc agcaggagag cctgagcgag gcggcggccg tgacggcaac
       61 cggcaaagcc ctgcagcagg cgcagtatcg ctacaaggcc gggctggtca cgtatctcga
      121 agtcgcgacc accgagaaca cggctctgca ggcgcagctg accaatgtca acatccaact
      181 gcgccgcatg aacgcaagcg tactcctggt caaagccctg ggcggcgggt ggcagaacga
      241 gacgccgcgg caatgatgcg cagcagcgca cgcggctcgc cgcaaactaa cttcgcccaa
      301 gatttccagc cgcaaattcc cagttggcca gtttgtcgag caacgcattc acatagtcga
      361 cgcgacgatt ttgatagtca agatagtacg cgtgctccca tacgtcgatc gtcagcaacg
      421 gcttcaaccc ttcgctcagc ggcgtatgcg cattgcaggt cttgacgatc ttgagtttcg
      481 cgccatcctg caccagccac gcccagccgc tgccgaactg cccaacggca gctttggaca
      541 attccttctt gcagacatcg acgccgccga aggatgcgtc catcaagccc ttgagagcgg
      601 ccggtggctc cccgccgcct tgcggccgta agctgtgcca gtaaaacgtg tgattccacg
      661 cctgcgccgc attgttgaaa atctcagcgt gctgcgcctg gccggccgtt gccctgacga
      721 tcttctccaa cggcatgtcc gcgtactcgg tgcccgcgat cagtttattg agggtatcga
      781 cgtaggtctt atgatgttta ccgtagtgga aaccgagcgt attcgccgaa attaccggct
      841 gcagtgcgct ctgctcgtac ggcagtgcgg gcagcgcgat cggcacagaa gaaaaaatgc
      901 ttgcggcgtt catatggtaa ttcctcgcgg gggttagcgt tgacttgctg caagcctagc
      961 cagcaaaccc tcccgtcgcc atcggcaacc atcaccggga tccgtaggcg tgcgctgacg
     1021 acttaaagca cgtacgggag caccatgcgc gcgggtcgtg ctcgcaccac cgcgaacggc
     1081 gctacgcgcg acgaaacgtg cgtggctgcg gcgccggccg cgcacagtcg cacgtcgcca
     1141 ctcttggtgt cgatccgcac cctgccgcgc ccatcgccat ttgtgaactg cagacgcgat
     1201 cccgggccat agcgtgattc aatcggtttc ggcccaaagc agttcttgat gtcgccgctg
     1261 atagtttgca cgtcgaactc cgcggacggc atggcagaga acttgaaatt aagatcgccg
     1321 ctgaccattt cagcctcgat tcgcgcatcg gccgccaagc cgagcgcagc cgtaatgttg
     1381 cccgagatcg ccttgaagcg gccgccagtc actgcgccaa gttcgacgct ttcggtgccg
     1441 ctcaccgtcg tgatgtctac ttcgccgcca cctcccgtga ggtggatatt gccgctgatg
     1501 gttttaacgt cgatcctctt ggcggctcgc gccgtcatac gcacatcgcc gctgacggtg
     1561 ctggcacgca ggtcgccgcc gacgtcgccg ctcaaattac cgctcaccgc ctgcagcttc
     1621 tgatccccaa gcacaccgga caccttgaaa tcagcgctga cgagggttgc cgtgaccgcg
     1681 cttctcgcgg ggatatggat cacgaggcgc gcctcgccgt ccgaactcca cgaatgaatc
     1741 gcgcgcgaca ccactcgaat ggaggtgcgc cggccagcgc tcgtcacatc gacccgctcg
     1801 acgttgtcgc ctgccgtgcc tgagacgtcc acctcgcttc gatcccaacc gtcaacctcg
     1861 acacttcccg agacgttgat gatttcgact tcgccctgcg ggtcggccgc acgatgctcc
     1921 tcgatggttc ttcccgcatg aacgagagac caaggcacga tcagtgctgc cgcaccaaat
     1981 accgtccagc cggcgtagca agtcttgttg tgagtcttcg acatgggcta tgtcctcgtc
     2041 atgggttgag tcgcctgcac tacgttgtcg tacaggtcaa gttcgtcgcg ccaggtgctc
     2101 tgccaaagct gttcgagcac cgggctcgca ggatcgctcg ccagagcctt gcgtatgtcc
     2161 tcatgcgctt ggcgaatgac cgcgagactc gcctcgattc gagctcgagt cgccggatcg
     2221 agcagcttca gccgctcacg aaagttgagc tcgagcgaat cgcgcgcggc aacgtagcgt
     2281 ggatttacgg gctcatcgaa gcttcgcacc tgcgccgcca gcggcaccgc gggcgatccg
     2341 gggtgggagc gcagcacagc ccaagtgagt gcgctcgcga tacaggcccc ggccgcgccc
     2401 gccgcgacag caaacgccac gctgcgcggg cggttgggcc gccgcggcag gcgccgcgca
     2461 atgcccggcc acagattgcg cggcggcgcg atggcgcgcg gcatatgcgc aagttgctca
     2521 tccagagagg gacgcatggg atcgttcatg agtgctccaa tccgagcgcg gcggccagca
     2581 gaccgcgcgc acgatgcagt tgtgccttac acgtgccttc ggcgatgccc aacatgcggc
     2641 ccgcttccat atggctgaag ccataaatcc ccaccaaaac cagcacgtcc cgagccccgg
     2701 ccggcaggcg ggcgattgca cgctcgaggt cgagcggcgc accatcgccg gcggggtctg
     2761 ccacctccgg cagacccgcg gcaggtgccg ccacctcgta ctccaggcgc aatcctctgc
     2821 ggcgcgcgag cacggtatgc acggcgatgc ggtgcagcca ggtggagaag cggctgcgat
     2881 gctcgaaacc agccaacgcg cgccaagcag cgacaaacga ttcctgcgtg caatcctcgg
     2941 cggcttcgcg ctgcccggtg aggcgcaggc acaagccgta cacacgcggc gcaaattgcc
     3001 ggtacaagct ctcgaaggca gccaggtcac ccgcgacggc ggcggctatc aggccctgcg
     3061 gctcagcagc atcacttgct tctgcggctt gcagcatccg ggccgtcccc acgttggttc
     3121 ccccaatggc attgagtatg ccgattagag gcgagcaggc tcgcgaaggt ttaaatgggc
     3181 ggcaagatca gcgcttgact tcttcgacca cggcctgacc cgagacccgc gcctgtatca
     3241 atgcccaaca aagtcccagc gtcagcaaac atgcgagcat gaacaccgcg acccaaatca
     3301 cggaattgtg gttcgacagg ctgaaccagc cccagctcga aaacagccac gccatcgcca
     3361 cgaagaaggc cacgcccaaa gccactccga acgttccgag tgagcgccat gtcgcgtgca
     3421 gaaaaaacag ccaagccgcc agcagcgcga tgcccaccac cagttgcagc ggttgcagat
     3481 gcggaaaatt accggcaatc cactggatga aagagtagcc gcttggattg aaagtcgcag
     3541 cgaccaggat gagcgaggcg agaaagcgcc ccatcaaacc accgatgctg agatttctca
     3601 tgaccgtctc cgacaatgat gcgcgcgcat ccatgcgcgc gacttttctc gaattgacac
     3661 cctgacgatc gcatccgtgc tgccgggtgt cgctccgtat tatctagatc ccgctgctgc
     3721 catggagtgc cagaccccgc agctcagttg agattatgtt agatattcgc accaaccgca
     3781 ccttgcgtcg atcataatat tcttgcgtgc ctgacggtcg atcaagaatc gccgcgggct
     3841 ttgtaaagtc cggcaaattc atttgagctc tatctccacg aaacgaaact cgtagtcatt
     3901 ggcattgatg acgttgtgtg cgactccggc ggggcgggca tacgacactc ccgcctgcag
     3961 agatgcgtcg cgcgtgcctt gcgcgtccac caggcgcagc gtgccgctgg tgagcggcac
     4021 caccacatag tcatgggcgt gcacgtgatg tccggtatcc gcgcccgggg cgaagcgcca
     4081 ctcggtcacg atcacgcgct cgttgtggac ttgcagcgtg ccggtcgctt tcatgtcatc
     4141 gcctctgcat tggatttggg agcaggcgtg caagcctagc tgttttcaaa agaaagaata
     4201 accccctcgg ccgcgtgcta acgtctcgct caacggcttt atacaggagg ctttcatggg
     4261 catgatgcgc gtagtcgccg cgagtgccgc ggcgctgact tggctttgcg gcgccggcgc
     4321 gcaggcatca ccgcagctca acgacagcgt atatcgggcg gtcctcgcca atcgtgccgc
     4381 cgcgctcgaa ctgctcgagc agattgtgga catcgattcc ggtaccgggg atgtcgccgg
     4441 cggtacccag gtgcaggccg tcatcgcggg acgcctgaag gcgctcggcg cgcaggtgag
     4501 cactgcaccg gccgaagcgc cgggactacc cgataatcta ctcgcggtat ttcacggaac
     4561 cggtaagggc aaactgctca tcatcgccca catcgacacc gtgttcgcac ccggaacggc
     4621 ggcgcaccgg ccgttcaaga tggatcaaca acgcgccttt gggccgggcg tcggcgacga
     4681 aaaagccggc gtggtgaacg cactgacggc gttgacgatt ctgcatgata tcgggttcaa
     4741 gaattacgcg accattacct tgctgctcga cgacagcgag gaacgcggtt cgccgggatc
     4801 ccggcaattg atcacgtcct tggtaaaggc gcatcaggtc gaattcaaca tggaacctgg
     4861 cgatccgcct gacgccctca ccgtgtggcg caagggctcg gccagcattc gcgttctggt
     4921 caatggacgc gcggcgcatg cggggatggc gccgcaggac gggcgcaatg cggcggtgga
     4981 acttgtgcac caactcgcgg gcttggaagg cgcattcccc cactccggcg ccggcaccac
     5041 ggtcaatctg acgattctga aatccggcga gcgcaacaat attatcccgg acctcgccga
     5101 ggcgacgctc aatgtccgct accgcaagcc tgaggatctc gatgccgtgt tggcccgcgt
     5161 cgaaagcggc gctcgcacga cgctggtcga ggatacgcgg gtcacggtga cgcacgatcc
     5221 tgcctatccg ccgctcaccg aaaatgcgca gatcgacgct ctcgccgccc gggcgcaagc
     5281 gatctatgcg gaactcggca agagcatcgc attgtccggc aacggcggcg catccgagtc
     5341 cgcgctggcg atggccgagg gtactcccgc gctcgatgga cttgggtttg tggggggtga
     5401 ctttcacacc gatcacgaat ggatcgatct cggcagcgtt gtgcctcggc tctatctttt
     5461 tacccggctt ttgatggaaa cgggagcgaa gccgccctga cctgggttaa ccttgtcgcc
     5521 gcatgccgcc ggtaattgtt tgcccagcct gtgatttggc gcatcgaact gctgcaggtc
     5581 cccgcaacgg gcgcatgcaa tgtgcccgct gccgtgcgcc gctgcaacgg ccggccaacg
     5641 cggcaatcga taccggaatc tcactggccg ctaccgcctt ggtgctgctg gtcttgagca
     5701 acctgtaccc cctggtggcg atgcacgtta acggcagttc ccgcgaaacg acgctgatcg
     5761 gcgccgcgcg cggcttgcac gcccaagggt tcgattcgct cgccgtgctc gtgctcatga
     5821 ccacggtgat cgccccgttg ctgcaaatag tgacgctgct gtatgttttg ctgccgttgc
     5881 gccgcggccg ccaagcacgc ggccagcacg ccgtgctgcg ggtgctcacg caaatccggc
     5941 cgtggagctt catggaggtc ttcatgctgg gtgcgctggt cgcgctggtg cggctgtcga
     6001 aattcgccga catcgttccc gggatctcgt tatggacatc ggtgctgttc atgctgtcgc
     6061 tgtccgcgct gaccagcatc acctcgcccg aacaactctg gcgatgggtc gaagggagcc
     6121 gccgttgagc agtccggcga ccggccgaag cctcgggctg ctcgtctgcc ttagctgccg
     6181 cgcaaccgtg cgacgcgtgc aatcggtcta ttcgcgctgc cctcgttgcc atgcgcgcct
     6241 gcatgagcgc aagccgcaca gcttagccat cacgaccgcg ctggtgatcg ctgccgccgt
     6301 gctctatgtg ccggccaacg tgctgccggt catgagcaca cgcaccctca tcgacgatga
     6361 gagcgacacc atcatgagcg gcgtactggt gttgctgcac tcgggctcct ggccgatcgc
     6421 gatcctggta ttcatcgcca gcattgtcgt cccgatattg aaaatactgg cattgggagt
     6481 gctgctatat tccgcatggc gccgctcgac gcgccggcgt ttgcagcgca gtgagctgtt
     6541 tcgaatggta gaattcatcg gacgatggtc catgctcgac atttacgccg tttctctgct
     6601 ggccactctg gtgcagatcc agtcgttcgc ctccatcatc gtcggctggg gcgcgtggtc
     6661 gttcggcgcc gtcgtgattt tgaccttgct ggcggcgcgg actttcgacg aacggctgct
     6721 ctgggatact ccgccgcatg actgaaccgg cgaatcccga cttggaggag ctgccgagcg
     6781 cggtgcccgc ccaatcacgc tggtcgcggc taccgctgat atggatattg cccacggtgg
     6841 tcgtcttggc gggcgccttc gtcgtggtcc acgaaaagct ggcgcaagga accgccatcg
     6901 acatccaatt tcgcaatgcc gaggatctcg aggccaacaa gaccaagatc cgctacaaag
     6961 acgtggaaat cggtgaagtt cgcgatatcc acgtgtccga ggatcgcaat agtgtcatcg
     7021 tgaatgcgct gattcatcgc gacgccaccg actatctcgt gagcgacacg cgcttttggg
     7081 tggttaggcc gcgcgtctcg gcggccggag tcacgggctt ggggactctg gtgtccggcg
     7141 ccaatatcag cgtcgacgta ggcaagtcga cggcgcgccg ccgctacttc gtcggcctag
     7201 aggtaccgcc gatcgtcacc gcgaatctgc agggtcggga atttgttttg catgccgacg
     7261 atctcggttc gttgagcgtc ggctcgtcgg ttttctaccg gcatattacc gccggccaag
     7321 tggtagcgta taacttggac ccgagcggta ccagcgtgac gctcaaggtt ttcatcaaca
     7381 gtccgtttga cacgtacgtc accgccgcca cgcgcttctg gcaagcaagc ggtatcgaca
     7441 tgtcgctcaa ttccgatggc gtcaagctgc gcaccgagtc actgacggcc attcttcaag
     7501 gcggagtagc ctttcaagca ttgcccgggg gtgcgacgac gcccgtgccc aacgattcgt
     7561 cgttcaccct ctatcaagac gaagaccgcg cgatgcggcc ggtcgaaacc gaggtccgca
     7621 gcttcgtgat gtatttcaaa ggctcgctgc gcggtttgtc cgccggcgcg ccggttgaac
     7681 tgcacggtat cggcgtcggt gaagtcagga gcgtggacgt ggaatacgat cgtgacgccg
     7741 gatccttgag ctttcctgtc gccgtggata tttatccgca gcggattcgc ggccgcaagg
     7801 caagcagcgc tcgctccgcc aaggcgcagg acgaccccga aacctccagc cgccggctgg
     7861 tcgatagcct ggtggcgcac ggactgcggg cggagttgaa aaccggaaac ttattgaccg
     7921 gacagaagta tgtagacctc gatatgcatc tggacgtccc ggcggaaaag gtttggtgga
     7981 acgagcaacc ctcggtgttc ccgaccgtgt cgaacgggct cgacgaaata caggactcct
     8041 tgggaagcat tgcgcggcgg ctcggcaaag ttccattcga tcagatttcc tatcgactgc
     8101 tgtcgaccct gacgagcctc gagcagacgc tgaaaagtac cgaccagttg atgcggcatg
     8161 tcgacggtac cctggcgccg caagtcgaag caacgctggt ggaagcacag gcggccatga
     8221 agaacgccaa ggagatattg tcgcgggatg cgcctttgca aagcgatctc ggagccactc
     8281 tgctgcagct gtcgcgagcg gcgaaggcgg tctcggcgct ggtcgactac ctcgagcgcc
     8341 atccggaatc cctgctgcgc ggcaaaccgg gagactcgcc gtgaaattac cgttatcggc
     8401 attgtggttg ctgagcatgc tgtgcgcatg cgccgcaagc cgcccggatc gtttttatgt
     8461 actcagcgtg cagccgccgg gcgccgtgga tgcgcggaca atgcctgcca ccgaggtatc
     8521 actcaaggtg gcgttgccgt cgatgatcga tcgcggggaa atgatcatcc aaacttcggc
     8581 ggatggcgtc caggtactcg agcacgaacg atgggctgcg ccgctcaccg acatggtcac
     8641 ggagactttg gcgcgcgacc tcgagcggcg gcgagcggat ttgctggtgg ccggagccgg
     8701 cgcggttctc gctgccggtg cgctcgtcaa aatc
//
LOCUS       DASWZT010000477         1684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_8591, whole genome shotgun sequence.
ACCESSION   DASWZT010000477 DASWZT010000000
VERSION     DASWZT010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8591"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1684
                     /locus_tag="VGE92_13725"
     CDS             <1..>1684
                     /locus_tag="VGE92_13725"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1314935.1"
                     /translation="VLGGAKTAAALADTQIILPGGKSVRLSEIAEVRDGIAEVRSIAR
                     LNGRAATTFRVFKAKGASDVSVAHMVTAELEKIRTENPSVHMTSIYTSVDHTLRTYDS
                     ALSALIEGSLLAVVVVWFFLRNWRATLISSLAIPLSAIPTFAFMQWMGFTLNQITLLA
                     LSLVAGILVDDAIVEIENIVRHMRMGKNCYQAAIDAADEIGLAVVATSFTIVAVFLPV
                     SFMGGVSGQFFKQFGLTVAAAVLISLMVARLITPVLAAYALRSDALSVHHADGPVMAR
                     YLKLLQWCVKNRWKTIGAGFVFFVLSVASLALVPSGFIPPEDFANSELDIELPPGGTL
                     QDTARVSAAAAAILRKSPEVTDIIEFVGGDDGEIRNGFIFVNLVPRSKRQLTQKQWEQ
                     AMMPLLATVPDGHLNFSNGGGGRDIQLYLTGDDPPLVERTGRQLLAEVRALPEVRDAR
                     IKGDLPRPEIVVRPRLDVAAQLGVSVQSISQTIRIATLGDLPQNGAKFSLTDRQIPIR
                     VSLIESARQDLNTLENLPVPTASGASVPLKSVADLSFGEGPSAVRRYNQSRRV"
BASE COUNT          285 a          535 c          548 g          316 t
ORIGIN      
        1 gtgttgggcg gcgccaagac cgcggccgcc ctcgccgata cgcagatcat actgcccggc
       61 ggaaaatcgg tgcgcttgag cgaaattgcg gaggttcgcg acggcatcgc cgaagtgcgc
      121 agtatcgccc ggctcaatgg ccgggccgcg accacgtttc gcgtcttcaa ggccaagggc
      181 gcctcggatg tgagcgtggc gcacatggtg acggcggagc tcgagaaaat ccgcaccgag
      241 aatccgtccg tgcacatgac cagcatctac accagcgtcg atcatacgct gcggacctac
      301 gattcggcgc tgagcgcgct catcgaagga tctctgctcg cggtcgtcgt cgtgtggttt
      361 ttcctgcgca actggcgcgc cacgctgata tcctcgctcg ccatcccgct gtcggccatt
      421 ccgacgttcg cattcatgca gtggatgggc ttcacgctca accagatcac gctgctggca
      481 ctgtcgctgg tggccggcat cttggtcgac gatgccatcg tcgagatcga aaatatcgtg
      541 cgccacatgc gcatgggtaa gaattgctat caggccgcaa tcgatgccgc ggatgagatc
      601 ggcctcgcgg tggtggcgac ctcgttcacc atcgtcgcgg tgttcctgcc cgtgagtttc
      661 atgggtggcg tgagcggcca attcttcaag cagttcggct tgacggtcgc cgcggcggtg
      721 ctcatctctc tgatggtggc gcggctgatt acgccggtgc tggccgccta tgcgctgcgc
      781 agcgatgcgc tgagcgtgca ccacgccgac ggcccggtca tggcccgata cttgaagctg
      841 ctgcagtggt gcgtgaagaa ccgctggaaa accatcggcg cgggattcgt gttcttcgtg
      901 ctctccgtgg cctcgctcgc gctggtgccg agcggcttca tccctccgga agactttgcg
      961 aattcggaac tcgacatcga gttgccgccg ggcggcacat tgcaggatac cgcgcgcgtt
     1021 tcggccgcgg ctgccgcgat cttgcgaaaa tcgcccgaag tgaccgacat catcgagttc
     1081 gtgggcggcg atgacggcga gattcgcaac ggcttcatct tcgtgaacct cgtgccgcgc
     1141 tccaagcggc aactgacgca gaagcagtgg gagcaggcga tgatgccgct gctcgcgacg
     1201 gtgcccgacg ggcatttgaa tttctccaac ggtggcggcg gccgcgacat ccagctgtat
     1261 ctgaccggcg atgatccgcc gctggtcgaa cgcacggggc gccagctgct cgccgaggtg
     1321 cgcgcgctgc cggaagtgcg cgacgcgcgc atcaagggcg atttgccgcg gccggagatc
     1381 gtggtgcggc cgcggctcga tgtggccgcc cagcttggcg tcagcgtgca gagcatcagc
     1441 caaaccattc gcatcgcgac gctcggggat ctgccgcaaa acggcgccaa attctccctg
     1501 accgaccgtc aaatcccgat ccgtgtcagc ctcatcgaaa gcgcgcggca agatctcaac
     1561 acgctggaaa acctgcccgt gccgaccgcg tcgggtgcca gcgtgccgct caagtcggtc
     1621 gcggatttga gcttcggtga aggcccgtcg gcggtgcgcc gctacaacca gagccggcgc
     1681 gtat
//
LOCUS       DASWZT010000478         5193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_8596, whole genome shotgun sequence.
ACCESSION   DASWZT010000478 DASWZT010000000
VERSION     DASWZT010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8596"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="VGE92_13730"
     CDS             complement(<1..1144)
                     /locus_tag="VGE92_13730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY1314936.1"
                     /translation="MIILARSAGVAAIGMVIAACALRETQPPPPALPPTFAAQAAVGE
                     QEWPSKDWYGTFGSAELNSLIEQAAANNLDVQGARSRVLQADARARQAGAAILPSVDF
                     GANGNYLAGHSSGGSAHETDWAALMSASYEVDFWGKNRATARAANALAQASRADRDTL
                     ALTALAGVGDGYFEILALRERLDLAIANRDASQKLLQVVQARFDAGLSNPVELAAQKA
                     TLAAAALVIPELEQREVEALAALALLVGREPERFVVQSREFGELREPGVAAGIPSQLL
                     TRRPDIFMAEANMRAANADLTAARAALLPSLTLTLSGGVQNPALNAAVISLSGVGPTL
                     NLGAALAQPIFDGGRLRAVRAEAQAKQEELVLAYRAAILAALADVEN"
     gene            complement(1219..2571)
                     /locus_tag="VGE92_13735"
     CDS             complement(1219..2571)
                     /locus_tag="VGE92_13735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY1314937.1"
                     /translation="MRLSATFAALFLALTGVLMGTVLWIVNGTLTATLLRENDADIET
                     VRNGFRDEGIEEAVEVIKQRLGNPKYGRSIRPDSYMILESAQGEVLAGNLPPQPRRLG
                     VLYTPSRAASSPAGRRGEPSEMLLGRGTEIAPGAYLFVGRDIRPMLATRARIIDAFIL
                     VTIGAVVIAIGAGLYLGRRFAQRVDAVARACESIVSGRMNERIELHDGGREWDRLARV
                     INDMLNRIAALLENLRQVSSDVAHDLRTPLTRLRNRLEQAREESATTADHVGAISAAI
                     EDTDQILSIFGSLLSIAQVEAGSRLTKFAAVDLSGLCEKVFRIYGPVAEDYGHRLTAD
                     LAGDVVIRGDPELLTQMFTNLIENAIHHTPKGTTIGIALAAAAGDTVATVSDDGPGIA
                     KDQTEKVLQRFYRLSSSRAAPGHGLGLALVAAVVQLHGGKLTLEDAAPGLKVAIRFPA
                     "
     gene            complement(2591..3277)
                     /locus_tag="VGE92_13740"
     CDS             complement(2591..3277)
                     /locus_tag="VGE92_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY1314938.1"
                     /translation="MSPQSGKRLLLIEDDVEFATSLVSRLRESSYAVEHRCDGRQGLA
                     AALNEEFDALIVDRMLPGIDGLTLVKTLRQQKSTPVIYLTTMSGIDERVQGLEAGGDD
                     YLVKPFAFAELLARIRALTRRTTQPLARLTAGAIEMDLVKRTVTREGRAIDLVPQEFK
                     LLECLLKNVDRVVTRKMLLEKVWEVYFDPGTNVVESHISRLRSSLNSGFSSDPIQTVR
                     GEGYRLLAIK"
     gene            3481..4623
                     /locus_tag="VGE92_13745"
     CDS             3481..4623
                     /locus_tag="VGE92_13745"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEY1314939.1"
                     /translation="MCGELWVGNKPFLPFTRPTIDADSIAAVGEVFRSGQLASGPKVQ
                     AFEAALQTYLGGARHVRVMTSATAGLEMALEVAGVGEGHEVIVPAMSFAASANVVARV
                     RARPVFVDIDLYTRNLNVDLVSAAITARTRAIMPVHFAGLPVDQDSLYALARQHGLRV
                     IEDAAHAIGSRHRGRLIGSFGDLVVFSFHPNKNMTTTEGGAVSTADKNEADRLEQYRF
                     HGIKRNALGEIDVLFPGAKSNLTDVAAQIGIDQLRRLDGFNRRRLQLASQYFTELEGF
                     EPAVLPARGDSGHSWHMFQVLIDFPAQGMTRPEFQARMAERGIGIGVHYPCIPGLTYY
                     REQGYRAEDTPVAARVGRQTVTLPLFPAMDDEDVPRVCRELRDLLH"
     gene            complement(4648..>5193)
                     /locus_tag="VGE92_13750"
     CDS             complement(4648..>5193)
                     /locus_tag="VGE92_13750"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1314940.1"
                     /translation="PGYLVQTMHMSILKSASMAAIPWACATLCDLLVGGWLIDHLIGM
                     GRDETRVRKAVLLLGMFAGLAVVGATTTTDPAVALTWISIALSGLAAAAPVGWSLPSL
                     IAPQGGAGTVAGIMNFANNMLGALAPVVTGYLVDRTHSFAAAFLAAGLVLLAGMASFG
                     LLLGRIEPLPEPEAMRQRTRA"
BASE COUNT          989 a         1699 c         1578 g          927 t
ORIGIN      
        1 cgttttccac atccgccaac gcggcgagaa tcgcggcgcg atatgccagc accaattcct
       61 cttgtttggc ttgcgcctcg gcgcgcaccg cgcgcaagcg tccgccatca aagatcggct
      121 gcgcgagtgc ggcgccgaga ttcaaggtcg ggccgacgcc gctcaaactg atgaccgccg
      181 cattcagagc gggattttgc acgcctccgc tcaaggtcaa ggtcaaggat ggcaacagag
      241 cggcgcgcgc agccgtcagg tccgcgttgg ccgctcgcat attcgcctca gccatgaaga
      301 tgtccggccg gcgcgtcagc aattgcgagg gtattccggc tgccacgccc ggttccctga
      361 gttcgccgaa ctcacggctt tgcacgacaa aacgctcggg ctcgcggccc accagaagcg
      421 ccagcgcggc gagagcctcg acctctcgct gttcgagctc gggaatgacc agagcggctg
      481 ccgccagcgt cgccttttgc gcggccagct cgaccggatt cgacaagccc gcatcgaagc
      541 gcgcttgcac gacctgcagc agtttttgcg acgcatcgcg gttggctatg gcgagatcga
      601 gccgctcgcg aagcgccaga atctcaaaat atccatctcc cacaccggcc aacgcggtca
      661 gagccaacgt atcccgatcg gcacgagaag cctgcgcgag tgcgttcgcc gcgcgggctg
      721 tcgctcggtt cttaccccag aaatcgactt cgtagctcgc cgacatgagc gccgcccaat
      781 ccgtttcatg cgcgctgccg ccgctcgaat ggccggccaa gtagttgccg ttggcgccaa
      841 aatcgacgct cggcagaatg gcggcgccag cctggcgcgc gcgcgcgtcc gcctgcagca
      901 ctcgactgcg cgctccctgc acatcgagat tgttggcggc cgcctgctcg atcaaggagt
      961 tgagctcggc actaccgaac gtgccgtacc agtccttgct tggccattcc tgctccccca
     1021 cggccgcctg cgcggcaaaa gtcggcggca gagcgggcgg cggtggctgc gtttcgcgca
     1081 atgcgcaggc ggcgatgacc atgccgatgg cggccacgcc ggcgctccgg gcgaggatta
     1141 tcatggcaaa acgctagcac gcgacgccgg ccgtcatttg tacgaaatcg taatgcccgc
     1201 atgccgcaag agcgtctctc aagccgggaa tcggatggcc accttgagcc cgggagctgc
     1261 gtcctcgagc gtgagcttgc cgccgtgaag ctgcacgacg gcggccacca acgccaaacc
     1321 caggccgtga ccgggcgccg cgcggctgct gctcaagcgg tagaagcgct gcaatacctt
     1381 ctcggtctga tccttggcga ttccaggtcc gtcatcgctg accgtggcca ctgtgtcgcc
     1441 cgctgcggcg gccaaggcga ttcctatggt cgtgcctttt ggtgtgtgat gaatcgcatt
     1501 ctcgatcaga ttggtgaaca tctgagtcag aagttccgga tccccgcgaa tgacgacatc
     1561 gcccgcaaga tcagccgtca gccgatgccc atagtcctcc gccaccggcc cataaatccg
     1621 gaacaccttt tcgcaaaggc cgctaagatc gaccgcggcg aacttcgtca ggcggctacc
     1681 cgcttccact tgcgcaatgc tcagcagtga cccgaagatc gacaagattt gatccgtgtc
     1741 ctcgatggca gcggaaatcg caccgacatg gtccgctgtc gttgcggatt cttctctggc
     1801 ctgttccagc cgatttcgca agcgcgtcaa cggcgtgcgc agatcatgcg caacgtcgct
     1861 cgagacttgc cgcaaatttt ccagcagagc cgcgatgcga ttcagcatat cgttgatgac
     1921 gcgggccaat cggtcccact cacggccgcc atcgtgcaat tcgattcgct cattcatccg
     1981 cccggacacg atgctttcgc aggcgcgtgc caccgcgtcg actcgttgcg cgaagcgtcg
     2041 cccgagataa agacccgcac cgatggcaat cacaaccgcg ccgatcgtca ccaagatgaa
     2101 cgcgtcgatg atgcgggcgc gcgtggcaag catcggccgt atatcgcgtc ccacaaaaag
     2161 atacgccccc ggcgcaatct ccgtgccgcg gccgagcagc atttcggaag gctcgccgcg
     2221 acgcccggct ggcgaagagg ccgcgcgcga cggcgtgtat aacacgccca agcggcgtgg
     2281 ctgcggcggc agattgcccg ccagcacctc gccttgagcg gattcgagaa tcatgtagct
     2341 gtccggccgg atggagcggc cgtatttcgg atttccgagc cgctgcttga tcacctccac
     2401 cgcttcctcg atcccctcgt cgcgaaaacc gttgcgcacg gtctcgatat ccgcgtcgtt
     2461 ctcgcgaagc aacgtggccg tcagggtgcc attcacgatc cacagcaccg tgcccatgag
     2521 aacgccggtg agagccagga acaatgcggc gaacgtggcc gaaaggcgca cggtctgcga
     2581 ttttagaaag ttatttgatc gcaagcagcc gatacccttc gccgcggacc gtctggatcg
     2641 ggtcgctgga aaaccccgaa tttaaactcg agcgcaagcg gctgatatgg ctttcgacga
     2701 cattggttcc gggatcaaaa tagacttccc atactttctc gagcaacatc ttgcgagtca
     2761 ccacgcggtc cacgtttttc agaaggcatt cgagcaactt gaactcctgg ggcaccaggt
     2821 cgatggcgcg gccctcgcgt gtcacggtgc gtttcacgag gtccatttcg atcgcgccgg
     2881 cggtcagtcg cgcgagcggc tgcgtcgtgc gccgcgtcaa tgcgcgaata cgggcgagta
     2941 attcggcgaa agcgaacggc ttgaccaggt aatcatcgcc gccggcctcc aacccttgca
     3001 cgcgctcgtc gatgccgctc atggtcgtca aataaatcac cggggtgctt ttctgttggc
     3061 gcagcgtctt gaccagcgtc aagccatcga tgcccggcag catgcggtcc acgatcagcg
     3121 catcgaactc ttcgttgagt gcggcggcga gcccttggcg cccatcgcaa cggtgttcga
     3181 ccgcgtagct gctttcacgc aggcgcgaaa cgagcgaggt cgcgaactcg acatcgtctt
     3241 cgatgagcag caagcgtttg ccggactgtg gcgacacccg gccaatgcta ctccagtgcc
     3301 aacacccgcg ggcgcagcac cgacgtaagc gcttctatac caatttgtaa gctaaccgcg
     3361 gccgccaggg gcatagttcg gcactcattg cagactcgtt cgtcatactg accgtgggcg
     3421 caaagtgaag gcgtgaccgg cggctctaca gaagccgcgc gtttcgccta gaatagcctt
     3481 ttgtgcggag agctgtgggt gggcaacaag ccgtttctac cgtttacccg gccgacgatc
     3541 gatgcggaca gcattgcggc tgtcggcgag gtatttcgct cggggcagct ggcaagcggc
     3601 cctaaggtgc aggccttcga ggccgcgctg caaacttacc tgggcggggc gcgccacgta
     3661 cgcgtcatga cgtccgcaac ggccgggttg gaaatggcgc tcgaagtggc cggcgttggt
     3721 gagggtcacg aagtcatcgt acccgccatg agctttgccg caagtgccaa tgtggtcgcg
     3781 cgggtgcgcg cccgaccggt gttcgtcgac atcgacctgt atacccgcaa cctcaatgtc
     3841 gacctcgtga gcgccgcgat aactgcgcgg acgcgcgcca tcatgccggt gcattttgcg
     3901 gggttgccgg tggaccagga ctcgttgtat gcgctcgcgc gccagcacgg cttgcgcgtg
     3961 atcgaggacg ctgcgcatgc catcggctcg cgccatcgcg gccgtttgat cggcagcttc
     4021 ggcgatctcg tggtattcag ctttcatccc aacaagaata tgaccaccac cgagggtggc
     4081 gcggtctcga ccgccgacaa aaacgaggcc gacagactgg aacaatatcg atttcacggc
     4141 atcaagcgca atgccttggg tgagatcgac gtgctgttcc cgggcgccaa gagcaatctc
     4201 accgacgtgg ccgcgcaaat cgggatcgac cagctgcgcc gcctcgatgg cttcaaccgg
     4261 cggcgtttac agctcgcctc gcagtacttc acggaactcg agggattcga gcccgcggtg
     4321 ttgccggctc gcggcgactc cgggcacagc tggcacatgt tccaagtgct catcgatttt
     4381 cccgcccagg ggatgacgcg ccccgaattt caggcgcgaa tggcggaacg gggcatcggc
     4441 atcggtgtgc actacccgtg cattccgggg ctcacctact atcgcgaaca ggggtatcga
     4501 gccgaggaca cgccggtggc tgcccgcgtc ggcagacaga ccgtgacttt accgctattc
     4561 cccgccatgg acgacgagga cgtccccaga gtttgtcgcg aattacgcga cctactgcac
     4621 tagtagcgcg atcaattgac acggcggcta ggcccgcgtg cgctgtcgca tagcctcagg
     4681 ctcaggcagc ggctcgatcc gacccagcag caatccgaac gaggccatgc ctgccagcag
     4741 caccagaccc gccgccagga aagcagcggc gaacgagtgc gtgcgatcga ccaggtagcc
     4801 ggtgacgacg ggcgccagcg cgcccagcat attgttcgcg aaattcatga tgccggcgac
     4861 ggtgcctgca ccgccctgcg gcgcaatcaa cgagggcaag gaccaaccca cgggagccgc
     4921 cgccgcgagt ccgctcaagg cgatggaaat ccaggtcaac gccacggcgg ggtcggtggt
     4981 ggtggttgca ccaaccacgg caagaccggc gaacatcccg agcaatagca cggccttgcg
     5041 cacccgcgtc tcatcgcgtc ccattccgat caggtgatcg atcagccatc ctccgaccaa
     5101 aagatcgcac agcgtcgcgc aagcccacgg aattgccgcc atgcttgccg acttgagaat
     5161 gctcatgtgc atggtctgca ccaagtagcc ggg
//
LOCUS       DASWZT010000479         3063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_8599, whole genome shotgun sequence.
ACCESSION   DASWZT010000479 DASWZT010000000
VERSION     DASWZT010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8599"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1588)
                     /locus_tag="VGE92_13755"
     CDS             complement(<1..1588)
                     /locus_tag="VGE92_13755"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004784.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEY1314941.1"
                     /translation="MTTTKKTLRSVRSYGPKNKDGFIHRSWMKSQGFPADVFDGRPVI
                     GICNTWSELTPCNAGLRDIAEHVKRGVWEAGGLPLEFPAMSLGETQMRPTAMLFRNLL
                     AMEVEESIRGNPIDAVVLLGGCDKTTPGQVMGAASVDLPTVVVSTGPMLNGKFRGREL
                     GSGTDVWKFSEAVRAQQMSLDDFLSAESGMSRTPGTCMTMGSASTMACLMEAMGLALP
                     FNATAPAVDARRRRNAHETGRLIVGMAREDVRISTIMTRDAFENAIRVLAAIGGSTNA
                     VLHLLAFAGRLGVPLELSDFDRLSADVPLLVDLQPSGRFLMEDLHYAGGLPAVMKELG
                     PLLCRGAATVWGRPIGESFAAAECFNREVVRTLANPVSPSSGIWVLQGNLCPNGAVIK
                     PSAASPALFKHSGKAVVFESIEDLRARIDDPDLEVDENSVLVLKGCGPRGYPGMPEVG
                     NMPLPRKLLERGVRDMLRISDARMSGTAFGTVVLHVAPEADAGGPLALVQNGDEILLD
                     GPARRLELLVTAQELAARAAL"
     gene            complement(1695..2615)
                     /locus_tag="VGE92_13760"
     CDS             complement(1695..2615)
                     /locus_tag="VGE92_13760"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEY1314942.1"
                     /translation="MPGLDLQGISRSVVKLRADVPEDAFTAGILGSQRTGNGVVINSD
                     GLILTIGYLVTEATDVWLTIPDGREVAAHPLAFDQVTGFGLVLPLEKVDIAPIPLGSS
                     ADIRAGDQAYVVSSPQFAEPQPVDVFARREFAGAWEYLLEDAIFATPAHPHWSGTALI
                     DGGGSLVGLGSLLVRELTDGAEINANMFVPIDLLKPILEELTTRGRASRQPRPWLGVY
                     AAEMTGKIYVTGVANGGPAQRADIREGDLIMRVADHDVATLPDFYRRLWAVGPAGSGV
                     PLTAIRGGTRLNLNVRSVDRLDLLKRPQKH"
     gene            complement(2640..>3063)
                     /locus_tag="VGE92_13765"
     CDS             complement(2640..>3063)
                     /locus_tag="VGE92_13765"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY1314943.1"
                     /translation="LLQRTIREGYETETADGRPAASLMGKRLGFDQGRMYLSFSPFTQ
                     LVATNDFAVLFLFTPRSTMATDVDLYWLVDGKASEVDVKRMIWGWDETTKQDKEITEN
                     NQAGILSKEYRPGRYSEHERRVVTFQQWYLAQFGHSLA"
BASE COUNT          591 a         1010 c          923 g          539 t
ORIGIN      
        1 agagggcggc gcgcgccgcc agctcctgcg ccgtgaccaa cagttcgagg cggcgagctg
       61 gaccgtcgag aagaatttca tcgccgttct gcaccagcgc cagcgggccg ccggcatcgg
      121 cctcgggagc cacgtgcagc accacggtcc caaaggccgt gccgctcatc cgggcgtcgg
      181 agattcgcag catatcccgc acgccgcgtt ccaacagctt gcgcggcagc ggcatgttgc
      241 cgacctcggg catgcccgga taacccctcg ggccgcatcc tttcagcacc agcacggaat
      301 tttcatcgac ctcaagatcc ggatcatcga tgcgcgcgcg cagatcctcg atcgactcga
      361 agaccacggc cttgccgcta tgcttgaaga gggccggact cgccgcgctc ggtttgatga
      421 cggcaccatt gggacagagg ttgccctgca acacccagat tccggaagac ggacttacgg
      481 gattcgccag ggtgcgtacg acttcgcggt tgaagcattc ggcggccgcg aaactctcgc
      541 caatcggccg accccacacc gtggccgcgc cgcggcacag cagcgggcca agctccttca
      601 tcacggcggg caggccgccc gcgtagtgca aatcctccat gagaaaccgc cccgaaggtt
      661 gcaggtcgac cagcagcgga acatccgccg ataagcggtc gaagtccgac agttccaacg
      721 gcactccgag gcggccggca aatgccaaca ggtgcagcac ggcgttggtc gagccgccga
      781 tcgcggccag gacgcggatt gcgttttcga aggcatcccg ggtcatgatc gtcgagatcc
      841 gaacgtcctc gcgcgccatc ccgacgatca agcgtccggt ctcgtgcgca tttctgcggc
      901 ggcgagcgtc gaccgccgga gccgtggcgt tgaagggcag cgcgaggccc atggcctcca
      961 tcagacacgc catggtcgat gcgcttccca tggtcatgca ggtgccgggg gttcgcgaca
     1021 ttcccgactc cgccgacaaa aaatcgtcga gcgacatctg ctgcgcccgc accgcctcgc
     1081 tgaacttcca cacgtcggtg ccggagccga gctcgcggcc gcggaatttg ccgttgagca
     1141 tgggacccgt ggagacgacc acggtcggca gatcgacact ggcggcgccc atcacctggc
     1201 ccggcgtcgt cttatcgcag ccgccgagca gcaccaccgc atcgatcgga ttgccgcgaa
     1261 tcgattcttc gacttccatg gcgagcaaat tgcgaaacag catcgcggtg gggcgcattt
     1321 gtgtctcgcc gagcgacatg gcgggaaatt ccagcggcag gccaccggct tcccaaacgc
     1381 cgcgcttgac gtgttcggca atgtcgcgca gcccagcatt gcaaggcgtg agttccgacc
     1441 aggtgttgca gattccgatc accgggcggc cgtcgaagac atcggcgggg aagccctggc
     1501 tcttcatcca gctgcggtga atgaagccat ctttgttctt cggaccgtag ctgcggacgc
     1561 tgcgcaacgt cttcttggtc gtcgtcatcg gttcgccccc gcaaaacagc cagcgagcgc
     1621 agtttatgct aagttataac attaatgtga tttatcggga atgcacactc gcgcatcgcg
     1681 aagtcgatcg ccgcttagtg tttttgcgga cgcttcagca ggtccagccg atcgaccgag
     1741 cgcacgttca aattcaggcg cgtgccgccg cgaatcgcgg tgagcggcac gccgctgccc
     1801 gcgggcccga ccgcccagag acggcgatag aagtccggta gggtggccac atcgtgatcc
     1861 gccacgcgca tgatcaaatc gccctcacga atatcggccc tttgcgcggg cccgccgttg
     1921 gcgacgccgg tgacgtagat cttgccggtc atttccgcgg cgtaaacgcc gagccacggg
     1981 cgcggttgtc gcgacgcacg gccgcgagtg gtcagctcct ccagaatggg tttcaacaag
     2041 tcgatgggca cgaacatatt ggcgtttatt tccgcgccat cggtcagttc gcgcaccagc
     2101 agcgacccta agcccaccaa actaccgccg ccgtcgatca gcgccgtacc cgaccaatgc
     2161 ggatgcgcgg gggttgcgaa tattgcatcc tccagcagat attcccaggc cccggcgaat
     2221 tcgcggcggg cgaagacatc cacgggctgc ggttcggcga actgcggcga actcacgaca
     2281 taggcttgat cgcccgctcg aatgtcggcc gacgatccta aaggaatcgg tgcaatgtcg
     2341 accttctcga gcggcagaac caagccgaag ccggtgacct gatcgaaggc gagcggatgg
     2401 gcggccactt cgcgtccgtc cgggatggtc agccaaacat cggtggcctc cgtcaccaag
     2461 tagccaatgg tcaaaatcag gccgtcggaa ttgatgacca cgccgttgcc cgtgcgctgc
     2521 gatcccaaaa tgccggccgt aaacgcatct tccggcacat cggcacgcag cttgaccacg
     2581 gaccggctga taccctgcaa atcgagccct ggcatcgcag ctcctgtgtc agttgcgcat
     2641 catgcgagcg agtgtccaaa ctgtgccaaa taccactgct gaaaggtcac gactcgccgc
     2701 tcatgctccg agtagcggcc cggccgatac tccttggaca agatacccgc ctggttgttc
     2761 tcggtaatct ccttatcctg cttggtcgtt tcgtcccagc cccagatcat tcgcttgacg
     2821 tcgacttccg aggctttgcc gtccaccagc cagtacaggt ccacatccgt cgccatggtg
     2881 ctacgcggcg tgaatagaaa caatacggcg aaatcattgg tcgccaccag ctgggtaaaa
     2941 gggctaaagg acaggtacat gcggccttgg tcgaaaccga ggcgcttgcc catcaaagac
     3001 gctgccgggc ggccgtccgc ggtttccgtt tcgtagccct cgcgaatggt ccgctgcagc
     3061 agc
//
LOCUS       DASWZT010000480        23215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_18171, whole genome shotgun sequence.
ACCESSION   DASWZT010000480 DASWZT010000000
VERSION     DASWZT010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 23215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23215
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18171"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1428)
                     /gene="nagE"
                     /locus_tag="VGE92_13770"
     CDS             complement(<1..1428)
                     /gene="nagE"
                     /locus_tag="VGE92_13770"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: protein motif:HMM:TIGR01998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HEY1314944.1"
                     /translation="MNRHFGGLQQLGRALMLPIAVLPIAGLLLRVGQPDLLGWSAMAS
                     AGDAIFSNLGLLFAVGVGVGLARENHGAAGLAALVGFLVTTRALETLLAAAPGSLAKL
                     SIPVGLLSGIIAGVSYNRFSDITLPSYLSFFGGRRFVPIISGLAGLVLAALLGYAWPF
                     LERGMDATSQAILGAGSLGLFAYGVLNRILIVTGLHHILNNFAWFIVGDYHGATGDLK
                     RFFAGDPTAGAFMSGFFPVMMFGLPGACLAMYHTARRERRGAVAGLLLSLGLTSFLTG
                     VTEPVEFTFMFLAPVLYALHAVATGIAMVVMHLCGVRLGFSFSAGLFDYVLNFNHAER
                     PLLLLPIGAAYFGLYYIVFRVLIVRLNLATPGREIDDPGQPLAPLPTGNRAEDFITAL
                     GGAPNLIEVAACTTRLRLVVADNTSIDEASLKRLGARGILRSSLQGLQVVLGPIADQV
                     AGEIRAAWRSPSARAAAAASTPPRAH"
     gene            complement(1425..3974)
                     /gene="ptsP"
                     /locus_tag="VGE92_13775"
     CDS             complement(1425..3974)
                     /gene="ptsP"
                     /locus_tag="VGE92_13775"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEY1314945.1"
                     /translation="MSQLEILAPLDGWCSSLDEVPDPVFAGRMLGDGLAIDPTGDLLV
                     APCAGEIVTLPASAHAVSIRAADGIDILIHIGVDTVQLAGRGFGARVRPGDRVRAGDP
                     LIRFDLDVVARAAKSLMTPILVTPMEGLSLRKCRAAGAVRAGDRLFEVSGPAPRNPAA
                     LDAARAPGDAAAAIHTLNLQLRQGLHARPAALLAQRAKSFGAESSLSVHGRSANARSV
                     VAIMALGVRQGDALTIRARGASDAAEAVAGLVAAIEEALRLESAAGHAAAAPARTTPP
                     IAAGRPSIAGALSGVSAVGGFAVGQVARIERREIAVVETGAGAARERDALAEALRHVQ
                     TRLARLAETGAAARREIIGAHLEFLDDPDLNLAAQRLIADGKSAAFAWRSALRRSIAE
                     LQALGDPRLRERVDDLLDVEAHVLLALAGEARPMSYPLPEHAVLVAQDLLPSELTALD
                     RLRLDAICLGGGGATSHVAILAAGMEIPMLIGLGADLCQVATGTTVIVDADGGTLQTA
                     PSPAAVEQARSELESRRRLRAEARAMAQTECRALDGTRIEIFANLGSALDAAAAVANG
                     AEGCGLLRTEFLFIDRETAPDEAQQLDAYQAIAAVLGRKPLILRLMDVGGDKPLRYLA
                     LPAEDNPALGLRGVRTSLIYPDLLRTQLRAALRVQPRGCVRVLIPMITDVAEILAVRR
                     VMDELVAELGVAGGIEVGAMIETPAAALMAPALIREVDFLSIGSNDLTQYTLAMDRGH
                     PVLAARTDALHPAVLKLIAASAGAAVAAGKWVAVCGGVAADPAAVPILLGLGVRELSV
                     VPAAIPALKRQVRKLDIASCRDLALRCLELTSPAEVRAWVAQGTASLGDGQ"
     gene            complement(3978..5447)
                     /locus_tag="VGE92_13780"
     CDS             complement(3978..5447)
                     /locus_tag="VGE92_13780"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEY1314946.1"
                     /translation="MRYAASRHVRIVPEIEMPGHATAAIAAYPSLGSAGAAAGAAAPT
                     VSAKWGVHTHLFNLQPSTFEFLDHVLAEVISLFPGTAIHVGGDEAVKDEWIASPAVQA
                     RARQLGIQDPGALQAYFTQRIGRSLAARGRRLIGWDEILRPGLSKDAIVMSWHGVSGA
                     HTAAAAGNVALEDVYRFDPHASLSDAEQHRVLGLQANLWTEHMQTEARVQWMALPRAA
                     ALAEVGWSLASRRRWPDFLERLVPMFARYRALGLNYADSVFAPSAELTPDGTGFTLAL
                     QNQAQASAAGVGVIRYTLDGRDPAAQSIRYDTLLHEPRGAEIRAAAFAGTDQASRVLV
                     KSLGERAALRRDSHDLELCSNALGLLLLPAGANVPLAVDIMNPCWIDRGVDLSAGPRL
                     LAAVAALPFNYEIGADAAKLRVGDARSAAGELEIHADGCDTPALATLPLAAAAHSRDV
                     TTLPALNLPRLPGKHDLCLRFARPRLDPLWALDWVEIGG"
     gene            5768..6511
                     /locus_tag="VGE92_13785"
     CDS             5768..6511
                     /locus_tag="VGE92_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY1314947.1"
                     /translation="MTSLAKAVGPLDGRGNLPLYQELQRALRKAIDSRILGPDDALPS
                     ERDLAADFAVSRITVRKALDGLVADGLLVRRQGAGNFVSARVDKNFAMLTSFSEDMRA
                     RGRTPRSVWLKRAAGTVTPEEALALRLSPGTPVFRFNRIRYADDAPMALEYATIVATA
                     LPSLEAVDTSLYEALERAGQRPVRALQRLRAVLLTGDQAELLKAKPGDAGLQVERLGS
                     LPDGRAIEFSQSFYRGDTYDFVAELSAST"
     gene            6508..7551
                     /locus_tag="VGE92_13790"
     CDS             6508..7551
                     /locus_tag="VGE92_13790"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY1314948.1"
                     /translation="MTRVDAAVEDTRMFQEAASASDAVREQLRCDGAAIAAIGAQLRR
                     LAPRAVVTCARGSSDHAATYAKYLIETRAGVLTASAAPSVSSVYGVSQDLSGCLFIAL
                     SQSGRSPDLLASVAAAKAAGATILALCNSSDAPLVATADWVIELRAGAETSIAATKSY
                     LATLAALLRLVAAWTRNAALEANIATLPRLMDQSWALDWSAALPPLESAAHLFVVGRG
                     LGLGAAQETALKCKETCGLHAEAFSSAELRHGPYALLGPGFPALLFTQRDATQAGVET
                     LAAELVRRGVPLLMAGARVAGAITLPTLDSSAEIAPLLLVQSAYRMIATLALRRGFDP
                     DHPAHLRKVTETV"
     gene            7551..8690
                     /gene="nagA"
                     /locus_tag="VGE92_13795"
     CDS             7551..8690
                     /gene="nagA"
                     /locus_tag="VGE92_13795"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEY1314949.1"
                     /translation="MRSALVNGRLLTGKGIVSGRTLLICGARIEALVDPKDRRCRDAE
                     RVDLQGQLLLPGFIDVQVNGGGGVLFNDDPSRESIGAIGAAHRRFGTTGFLPTLISDD
                     LDTIGRAIDAVQSALDAGTPGVLGIHIEGPFLNATRRGVHDAKHLRLLDADSIAQLSR
                     LRGGRTVLTLAPEMTTPGVIAQLSARGILVSAGHSDANFAETMAAIAHGLKGFTHLFN
                     AMARLEPRDPGIVGAALYDENTWCGIIVDGHHVDPIMLKLALRCKRHDRFMLVTDGMP
                     AVGSSEPTFVLQGRTIRVVDGICRDENGTLAGTALDMAAAVRNAVSLLGLDIVEAARM
                     ASEYPAEFLGLGAELGRIEPGYRANLVQLDDEFRVQKTWIEGLAV"
     gene            complement(8692..9288)
                     /locus_tag="VGE92_13800"
     CDS             complement(8692..9288)
                     /locus_tag="VGE92_13800"
                     /inference="COORDINATES: protein motif:HMM:NF018151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeA/HdeB family chaperone"
                     /protein_id="HEY1314950.1"
                     /translation="MKAVVALAFVLGLLPCAGRAGTLNLATLSCGKYENEVLPAAATN
                     PTADSINTVMWLFGYSVAKSGAHVMYPDALAPFGFALDNECKSNPAESMLDALNIVKP
                     EAKNPMDLSAVECSSFAARHAEFRRSDPESATTIAMWLYGFSVATSGSHLFNADAVGA
                     FETSLLAYCGEHPSISLLDTLKALQSKAPKAAKPAPHP"
     gene            complement(9285..10142)
                     /locus_tag="VGE92_13805"
     CDS             complement(9285..10142)
                     /locus_tag="VGE92_13805"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEY1314951.1"
                     /translation="MTCKPELLKSVPLFALLDDDETAVLAGQVELKTFASRERIYKMG
                     APGGQAYVMISGKVRVCIVDDDGQEVVVDEPQVGEFFGFASMLEQTPHRTNAIALEDT
                     SCIEVDRDDIAVLLQRKPHAGMDLLTVLGRQFHAAQELARLRASRNPNDIIEKEATFG
                     ERIADTVAGFGGSWTFISLFGIVLTVYTLINIVLGKSAWDPYPFILLNLFLSMLAAIQ
                     APVIMMSQNRQNTRDRLRSELDFEVNRRAESEIQGIARKLNLLDDKMHDFDDLLRLAA
                     RANVQSLSK"
     gene            complement(10242..10895)
                     /locus_tag="VGE92_13810"
     CDS             complement(10242..10895)
                     /locus_tag="VGE92_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY1314952.1"
                     /translation="MPHSSNPHSTPAEPAPAARVRIIEERVLSDDWYLLKKTRFEYLR
                     RDGRRQEQVRETYDRGNGAVCLLFNVERDSVVLIRQFRFPTYVNGSADGLLIEACAGL
                     LDGDDPLTCIRKEAQEETGYIVSSPHKVFEAYMSPGSVTEKLHFFVAEYRDEARTSDG
                     GGDAGEGEDIEVLELPLATALQMIDSGAIQDGKTIMLLQRAGMAGLARLRSMCRADP"
     gene            10933..11691
                     /locus_tag="VGE92_13815"
     CDS             10933..11691
                     /locus_tag="VGE92_13815"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEY1314953.1"
                     /translation="MLTAQRKKLLLARLAADGQIIAVELARELGTSEDTIRRDLRELA
                     KAGKLQRVHGGALPASAAVGDLHEREQVSPQEKIALGRLGAAMIRPGQVVILDGGTTA
                     LQVARHLDPELRATLVTHSPTVAVELAAHPAIEIIVLGGRLFRHSMVSVGAALIEAAA
                     QLRADLYFMGVTGVHPEAGLSTGDAEEAAVKRALHERAAETIVLASSEKIMAASAFVV
                     APLKSLSLLVVPAKTPERTLRALRAGGSKVRRAP"
     gene            11769..13052
                     /locus_tag="VGE92_13820"
     CDS             11769..13052
                     /locus_tag="VGE92_13820"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1314954.1"
                     /translation="MRTVVAASAIGTTIEWYDFLIYSTAASLVFNKLFFPTGDPLTGK
                     LLAIATIGVGFFVRPLGAIVLSHFGDRVGRKSMLIVTLVTMGVGTMLIGLLPTYQSIG
                     VAAPMLLVACRVAQGIAVGGEWGGAVLMVIEHSPPNRRGFYGSIVQIGFPLGMAIGTA
                     SFFALARLDEAEFMSWGWRLPFLASALLVAVGTFIRLRIDETPDFKRNVRDGHIARFP
                     VLDTLRRHPKDLLIGLGARITEVSWIYVITIFGLSYAVTNIGLPRGLVLGAIAAGAAV
                     ELITIPVCGALADRFGRRPIYMLGCLAAIALSFPIFWAIQTRDPLTVVLGFVIGMSVG
                     HGIMYGVQASFLSEMFPANLRYSGASLGYQLAAPIGGGLIPVIAAAIVGRNHGATWPV
                     STLMIALALVTMLAVVYAKETAPALATTRGTHVRG"
     gene            complement(13037..13741)
                     /locus_tag="VGE92_13825"
     CDS             complement(13037..13741)
                     /locus_tag="VGE92_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314955.1"
                     /translation="MSDRVSGPHCVVRLGRNWIDSGTLARTRSSRPGPTQPIERSEFS
                     ASIERLQTLLQDPDGILTRLASARSPDASSQLLLFLQTLEKQIRNFLGRPALRVTGST
                     ERWLRGSDSIAEKGQTEHVRDPQTGRRLTAYDQGAYLARTSARSASAVLPGWNKMDTI
                     EEQAVPARSIVLQGIAAPQNPGGAWAHGGTPLPGGGEQIFHAGQLSLDGRLLPPAERE
                     ILSSTRPDGSRIHPRT"
     gene            13928..15367
                     /locus_tag="VGE92_13830"
     CDS             13928..15367
                     /locus_tag="VGE92_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314956.1"
                     /translation="MRKDLIEHRKWLSRTAVIGKFRSTELKALDAALLAYEVSGSESD
                     LKLIRTTLAAWKQKEVKSGHPWEKSVRNKDGAFKELTERLDAEPVSNFYTQDLAKTRL
                     GVLYLFSGMDVESGMFEVILDGGLNFAGNVAGLGGALTASADGATAAAAIGTNIGIAQ
                     AISYEVLNATPASNTANNSKLSGILGPIKTQLETYAKQIWAHLTSMFKKDASKSWIDF
                     AGDWWSRITGVINTVLNQIGVSAAPFVGAAFDIVQGSARTVKACYDRYVTYSLSKGVD
                     MNDGHPSVVVKSIERTMNLAIGSGLYQALKGAVTTGVNVVGSGAGAILSMVVSGCELI
                     AKVVYRLWESSKMKAFFEDCKDRYTKFKTAKKVADDDVTHSGIAFGAWYRAAAYTLPC
                     LSALALCSGLTGDKMMYLSMFKDQKDLTAPAQTMNPISQSEFDRGVAYVDHLKEAAKD
                     YLSSSGYEFSSTDKVVDGMFKSKNFFGNK"
     gene            15494..16480
                     /gene="tssA"
                     /locus_tag="VGE92_13835"
     CDS             15494..16480
                     /gene="tssA"
                     /locus_tag="VGE92_13835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HEY1314957.1"
                     /translation="MAGMDINALVQPIGEGAPCGIDLEDTQQLAAIDAFRVFGQGTPL
                     AVDTDWRAINAAALEALGVSHDFRLLAHVSAAQLRLTGLQPFIDSLCVAARWLEDSFA
                     DVFPRIDDDAVLRKNALNYFADRMAIIDALRRAPLVSHRQLGSYTLRHMAIAAGQQTP
                     SEEEAAAPPTEDLINAAFEASPQEDLVQLVASIDTGLAALKAIEAAMVAEHGVQAAPD
                     TGPLAEVLTRMREPVAKHVRAPMIETAAEAVDGETAAAGVPGQIRSREDAVRSLDAVA
                     DFFRRSEPSSPVPMFVERAKRLISRDFLEVLAELAPESLAAVKHVGGIREPE"
     gene            16522..17034
                     /gene="tssB"
                     /locus_tag="VGE92_13840"
     CDS             16522..17034
                     /gene="tssB"
                     /locus_tag="VGE92_13840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HEY1314958.1"
                     /translation="MAKPSSQKFIARNRAPRVQIEYDVELYGAKKKVQVPFVMGVMSD
                     LSGDSKEPLPSVDERKFLQIDIDNFDDRMKSMKPRAAFTVPNTLTGEGNLQVDLTFES
                     MDDFSPAAVARKVDALAKLLEARSQLSNLVTYMDGKAGAEEMIAKVLNDPALLKSLAA
                     KAKPQDGGNS"
     gene            17054..18541
                     /gene="tssC"
                     /locus_tag="VGE92_13845"
     CDS             17054..18541
                     /gene="tssC"
                     /locus_tag="VGE92_13845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HEY1314959.1"
                     /translation="MAEAEKVAAPQVASLEADQFEALLAKEFKPKTDRARAEVEGAVR
                     TLAEQALRESTVVSDDVIGTIKAIIAEIDKKLSDQMNLILHSERFQQIESAWRGLNYL
                     VSNTETDEMLKIRVLNISKKDLAKTLKKYKGVAWDQSPIFKRLYEQEYGQLGGEPYGA
                     LVGDYYFDHTPGDVELLGQIAQTAAAAHAPFLAAAAPSLMGMDSWQELSNPRDLAKIF
                     SAPDYAAFRSLRESDDSKYIGLAMPRFLSRVPYGAKTSPVEEFDFEEETGAGDQNKYT
                     WTNAAYAMAVNVNRSFKQYGWCTSIRGVESGGAVEGLPVHTFPSDDGGVDMTCPTEIA
                     ISDRREMELANSGLMPLIHRKNTDSAAFIGAQSLQKPTEYDDPDATANARIAARLPYL
                     FASCRFAHYLKCMVRDKVGSFMEREDLERYLHSWIQNYVLGDPSRAGDTLRAQKPLAA
                     AEVKVEEIEGNPGFYAATFHLRPHYQLEGVNVSMRLVSKLPSQKK"
     gene            18631..19137
                     /locus_tag="VGE92_13850"
     CDS             18631..19137
                     /locus_tag="VGE92_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018927416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HEY1314960.1"
                     /translation="MAMDMFLTFDEGAKIKGETIDKTYKAKHGIDVLAWSWGISNSGS
                     AHVGQGAGSGKANFQDISFTKYIDLASCALMDACSLGTHIKEANLIVRKSSGDKKEPL
                     EYLTMKLETVLVSSYSTGGSGGEDRLTENVSLNFAKVTVLYKQQAATGVAGEKGNFTY
                     DIPANAQT"
     gene            19211..20008
                     /locus_tag="VGE92_13855"
     CDS             19211..20008
                     /locus_tag="VGE92_13855"
                     /inference="COORDINATES: protein motif:HMM:NF018699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
                     /protein_id="HEY1314961.1"
                     /translation="MATAEESFRAGDLAGCLALLQAEVRRRPEDVRQRIFLVQVLMVL
                     GEWERALTQLGVLKEMDASALPMVHSYGAAIQCERLRAAVFRGERSPLLFGEPEPWMA
                     EMVQALGLQARGHASEAAALRSRALEAAPASAGTLDGQAFEWLADADPRLGPLFEVLL
                     NANYYWVPMHRVAEIRIEAPSDIRDLVWLPAEFVWANGGEAVGFIPTRYPGSESNTDD
                     AIRLARKTDWVGEGDQSVGFGQRILASDAFEVPLLELRSVKFERPST"
     gene            20041..20820
                     /gene="tssE"
                     /locus_tag="VGE92_13860"
     CDS             20041..20820
                     /gene="tssE"
                     /locus_tag="VGE92_13860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HEY1314962.1"
                     /translation="MAELAFEDRLQPALLDRLSDDARSVLVVELVTSRARLAEHGASI
                     DAVEFALRPFGLRRWRADRDSPEAGAEDALVLQFSGPTDGATLTRVRATPVAATPQPL
                     SLSRLADVASRSIPNLNPEPAAQRVVSMRQLRESVLRDLSWLLATSSYDSDRSLAAWP
                     EVERSVFNYGLPSIAGHGLSSFDANAAAARLQKAVEAFEPRLAHVHVISEVQAGNMDR
                     NALVFRVEAELWGQPTPQRMLLRTQIDVESADVMITDLESG"
     gene            20820..22691
                     /gene="tssF"
                     /locus_tag="VGE92_13865"
     CDS             20820..22691
                     /gene="tssF"
                     /locus_tag="VGE92_13865"
                     /inference="COORDINATES: protein motif:HMM:NF017733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HEY1314963.1"
                     /translation="MDPRLLDYYNSELRFLRESGVEFARAYPRIAARLGMESLEVADP
                     YVERLIEAFAFLAARVQLKLDARHPQFTEHLLGVVHPSFLNPVPACGVAEFLPDLKEA
                     SLQAGVTIPRGSIMRTVLAKGERTASVFTSAHAVTLWPLAISEAKYLSGSGSLTGTGI
                     AVGSQVKAAIRLRLQASPGVKLNQLPIERLRFYIKATPDLAARLYEQIAANVTGYYVR
                     APGERSVKFCPAQAVRAAGFEEDEALLPTPRRGFEGYRLLQEYFAFPERFLFFDIDEL
                     RAGLADCAGGAAEIYLAFDRVQPGLENAVDHSQFRLNCTPIVNLFRHSPDRVHVDAAV
                     TEQHLVPDRNRPMDFEVYEVLRVTGVSASGEVLEEIHPLYLSSHQSGIERSRPYYSLQ
                     RRPRLLSTRQQQSGTRTNYVGTEVFVSLADARGRPVDAEITHLDVQALCTNRDLPIRS
                     AFGKGRTDFQLEAAAPLEGIRCIAGPSTPRTSPAFGDAAWNIISHLSLNYLSLIDADP
                     GQGALILRELLALYADPKDPAAARQIEGVTHVAYAPVVRRIPIPGPMSFARGLEMTLT
                     LDDAAFEGVGILTLASVLERFFARYVSLNSFAELKVRSSSRGEVKRWAVRTGTRQLL"
     gene            22688..>23215
                     /gene="tssG"
                     /locus_tag="VGE92_13870"
     CDS             22688..>23215
                     /gene="tssG"
                     /locus_tag="VGE92_13870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HEY1314964.1"
                     /translation="MNPLDRLREQPYGFSLFAALRLIEQAYPERPRLGEARRPGDDAV
                     RLAQPPHLIFAPSDVMGLGQTESGTARLEQYGFGVFGPNGALPLHLTEYAFERAAHRG
                     DPGVVDFINTFQHRFIALFYRAWADGDPSVNLDRPASDRFVRCLGALVGLAPPSSRAA
                     APLAAQAALGRAALFG"
BASE COUNT         4145 a         7410 c         7616 g         4044 t
ORIGIN      
        1 atgcgcgcgc ggcggcgtgg acgcggcagc ggcagcgcgt gcggagggcg agcgccaggc
       61 tgcgcgaatc tcgccggcaa cttgatcggc gatcggaccg agcaccacct gcaagccttg
      121 cagcgaggag cgcaggatgc cgcgggcgcc caagcgcttg agcgatgcct cgtcgatcga
      181 ggtgttgtcg gccaccacca gccgcaatcg cgtggtgcag gcggcgactt cgatgaggtt
      241 gggcgcgccg ccgagcgcgg tgatgaagtc ctctgcgcga ttgcctgtcg gcagcggcgc
      301 gagcggctgt cccggatcgt cgatttcgcg gccgggggtc gcaagattca ggcgcacgat
      361 cagcacccga aacacgatgt agtacaggcc gaaataggcg gcgccgatcg gcagcagcag
      421 gagcgggcgt tcggcatgat tgaagttcag cacgtagtcg aacagacccg cggaaaagct
      481 gaagccgagg cgcacgccgc agaggtgcat cacgaccatc gcaatgccgg tggcaaccgc
      541 atgcagtgca tagagcaccg gagcaaggaa catgaaggtg aactcgaccg gttcggtcac
      601 gccggtcaaa aacgacgtca gcccgaggga taacagcagg cccgcgacgg cgccgcgccg
      661 ctcgcggcgc gccgtgtgat acatcgcaag acaggcgccc ggcagcccga acatcatgac
      721 cgggaagaat cccgacatga acgcgcctgc ggtcggatcg ccggcaaaga atcgcttcag
      781 atcgccggtc gcgccgtggt agtcgccgac gatgaaccag gcgaagttat tcaggatgtg
      841 atgcagcccg gtgacgatca atatccggtt caacacgccg taggcgaaca ggccgagcga
      901 gcctgccccc aagatggcct ggctggtggc gtccatcccg cgttcgagaa agggccaggc
      961 gtagccgagg agggcggcga gcacgagtcc ggccagcccg ctgataatgg ggacgaagcg
     1021 gcggccgccg aaaaaactca aatagctggg cagtgtgatg tcgctgaagc ggttgtagct
     1081 cacgcccgcg atgattccgg agagcaatcc gaccggaatg ctcagcttcg cgagcgagcc
     1141 gggcgccgct gccaacaggg tttccagcgc tcgggtcgtc accagaaatc ccaccagcgc
     1201 cgcgagcccc gccgcgccgt gattttcgcg cgcgagtccg acgcccacgc cgaccgcaaa
     1261 caacagtcca agattcgaga aaattgcatc gccggcggag gccatcgcgc tccacccgag
     1321 gaggtcgggc tgacccaccc gcaacaggag gccggcgatg ggcaacaccg cgatcggcaa
     1381 catcaatgcc cgacccagtt gctgcagtcc gccgaagtgg cgattcattg tccgtctccc
     1441 agggacgccg tgccctgcgc gacccaggcg cgcacctcgg ccggcgatgt cagttcgagg
     1501 cagcgcagcg cgaggtcgcg gcagctggcg atatcaagtt tgcggacctg gcgtttcagc
     1561 gccggtatcg ccgcgggcac gaccgagagt tcgcgcaccc cgagtccgag caatatcggc
     1621 accgcagccg gatccgcggc cactccgccg caaacggcga cccatttgcc ggccgcgacc
     1681 gcggcaccgg cgctcgccgc gatcagcttc aagaccgccg gatgcaaggc gtcggttcgg
     1741 gcggcaagca ctggatgacc ccgatccatg gccagagtgt actgcgtcag atcgttgctg
     1801 ccgattgata ggaaatcgac ctcgcggatg agggctggcg ccatcaaggc ggccgcaggc
     1861 gtttcgatca tggcgcccac ctcgatccct ccggccacgc cgagctccgc gacgagttcg
     1921 tccatcacgc ggcgcacggc gagtatctcc gccacgtcgg tgatcatcgg gatcagcaca
     1981 cgcacacagc cgcggggctg cacgcgtaag gccgcgcgca attgcgtgcg caagagatcc
     2041 ggatagataa gcgaggtgcg tacgccgcgc aagccaagcg ccggattgtc ctccgcaggc
     2101 agcgccaggt agcgcagcgg cttgtcgccg cccacgtcca tgagccgcag aatcagcggt
     2161 ttgcgcccga gcacggcggc gatggcctgg taggcgtcga gctgctgcgc ctcgtcgggg
     2221 gcggtttcgc gatcgatgaa gaggaattcg gtgcgcagca ggccgcagcc ctcagctcca
     2281 ttcgcgacgg ccgccgcggc gtccaaggcg cttcccaagt tggcgaagat ttcgattcgc
     2341 gtaccgtcga gcgcgcggca ctcggtctgc gccatcgctc tggcttcggc gcgaagccgg
     2401 cggcgcgact cgagctcgct gcgggcttgc tcaacggcag ccggcgacgg cgcagtctgc
     2461 agagtgccgc cgtccgcatc gacgatgacg gtggtgccgg tggcgacttg gcagagatct
     2521 gcgcccaagc cgatcagcat cgggatctcc atcccggctg cgagaatggc cacgtgcgag
     2581 gtggcgccgc cgccgccgag gcagatggcg tcgagcctga gccggtcgag cgccgtcagt
     2641 tccgaaggca gcaaatcttg ggcaaccagc acggcgtgct ccggcagggg atagctcatc
     2701 ggccgcgcct cgccggcaag cgccagcaac acatgcgctt ccacgtcgag caaatcatcg
     2761 actcgttcgc gcagccgcgg atcgccgagg gcctgaagtt cggcgatgga gcggcgcagc
     2821 gccgatcgcc aagcgaacgc ggcgctcttg ccatccgcga tcaagcgttg cgccgccagg
     2881 ttgagatcgg ggtcgtcgag aaactcgaga tgcgcgccga tgatttctcg gcgtgccgcg
     2941 gcgccggttt cggccaagcg cgcgaggcgc gtctgcacgt gacgcagcgc ctcggcgagt
     3001 gcatcccttt cccgtgcggc gcctgcgccg gtctcgacga cggcgatctc gcggcgctcg
     3061 attcgcgcga cttgccccac cgcgaagccg ccgacggcac tcactcccga cagcgcaccc
     3121 gcgatcgatg gccggcctgc cgcgatcggc ggcgtggtgc gcgcgggcgc cgctgcagcg
     3181 tggcccgctg cggattcgag gcgcagcgcc tcttcgatgg cggccacgag gccggccacc
     3241 gcctcggccg catcggaagc accgcgagcg cgaatcgtca atgcatcacc ctgacgcacg
     3301 ccgagcgcca tgatagcgac cacgctgcgc gcattcgcac tgcgaccatg cacgctgaga
     3361 gaggactctg cgccgaatga cttggcgcgt tgcgccagca gcgccgccgg ccgagcgtgc
     3421 aagccctggc gcagttgcaa gttcagggtg tgaatcgcgg ccgcagcatc gccgggggcg
     3481 cgagcggcgt cgagcgctgc gggattgcgc ggcgcgggac ctgagacttc gaacagccgg
     3541 tcgccggcgc gcacggcgcc agcggcgcga cacttgcgca gactcaggcc ttccatcggc
     3601 gtcactagaa tcggtgtcat caaactcttg gcggcgcgcg ccaccacgtc gagatcgaaa
     3661 cgaatcaatg ggtcgccggc acgaacacga tcgcccgggc gaacgcgcgc accgaagccg
     3721 cggccggcga gctgtaccgt gtcgacgccg atgtgaatca agatgtcgat tccgtccgcg
     3781 gctcggatcg agaccgcatg ggcgctcgcg ggcagcgtga cgatttcgcc ggcgcacggc
     3841 gcgaccagca ggtcgcccgt tggatcgatc gcgagaccgt cgccgagcat gcgtcccgca
     3901 aataccggat cgggtacttc gtccaggctg gaacaccagc cgtcgagcgg cgccaagatt
     3961 tcgagttgcg acattcgcta gcccccgatc tcgacccagt cgagcgccca gagcggatcg
     4021 agccgcggcc gtgcgaaacg caggcacagg tcgtgcttgc cggggaggcg aggcaggttg
     4081 agcgccggca acgtcgtcac gtcgcggctg tgggcggccg ccgccagcgg cagcgtcgcg
     4141 agcgccggcg tgtcgcagcc atcggcatga atctcgagct cacccgcggc gctgcgcgcg
     4201 tcgccgacgc gaagcttggc ggcgtcagcg ccgatttcat agttgaacgg cagcgctgcg
     4261 accgcggcga ggagccgcgg gcccgccgag aggtcgacgc cgcgatcgat ccagcacgga
     4321 ttcatgatgt cgacggcgag cgggacattc gcgccggcgg gcagcagcag caagccgagt
     4381 gcattgctgc agagttcgag atcatggctg tcgcggcgca gcgccgcgcg ttctcccagg
     4441 gatttgacca gcacgcggga tgcttgatcg gtgccggcga aggcagcggc gcgaatttcc
     4501 gcgccgcgcg gctcatgcag cagcgtgtcg tagcggatcg attgtgctgc cgggtcgcga
     4561 ccgtccaagg tgtagcgaat gacgcctacg cccgcggcgg aagcttgcgc ctgattctgc
     4621 agcgccagcg tgaagcccgt gccgtcgggc gtaagctcgg cagagggcgc aaacacgctg
     4681 tcggcataat tcaaacccaa ggctcgatag cgcgcaaaca tcggcaccag ccgttcgaga
     4741 aaatccggcc agcggcgccg cgacgccagc gaccaaccga cttcggcgag cgccgcggcg
     4801 cgcggcaacg ccatccattg aacgcgcgcc tcggtttgca tgtgttcggt ccagaggttc
     4861 gcctgcaggc cgagcacgcg atgttgctcg gcgtcgctca agcttgcatg cggatcgaag
     4921 cggtacacgt cttcgagcgc gacgttgccg gccgcggcgg ccgtgtgcgc cccggaaacg
     4981 ccgtgccagg acatgacgat ggcgtctttg ctcaatcccg ggcgcagaat ttcgtcccat
     5041 ccgatgaggc gccggccacg cgccgcgagg ctgcggccga tcctttgcgt aaagtaggcc
     5101 tgcagcgccc ccgggtcttg aatgccgagt tggcgggcgc gcgcctgcac ggcgggcgat
     5161 gcaatccatt catccttcac cgcctcatcg ccgccgacgt gaatggcggt gcccgggaat
     5221 aggctgatga cttctgccag cacatggtcg agaaactcga atgtcgatgg ctgcaaattg
     5281 aagaggtgag tatgcacgcc ccacttggcg gagactgtcg gtgcggcagc gcccgcggct
     5341 gcaccggccg aaccgagtga cggataggct gcgatcgccg ccgttgcgtg acccggcatt
     5401 tcgatctcgg gaacgatgcg cacgtggcgg ctcgccgcgt aacgcacgat ctcgcgaact
     5461 attcatacaa tgccccctgt ccttaacctg attcggtttg aaattggctg cgtcggtgtg
     5521 tggcggttgt tgtcgcgtcc cgaaaatcat cccgtcgagg cgtcgtccta tccacaccaa
     5581 ttcagaccca atatagtacc aatctgcgca atctgctagt gccgtgtaat aaatacaccc
     5641 aaaatcacat aatatgctat aaaaataatg agttacacga taataccagt ggttgttgtt
     5701 ctaaaagtgg tatttatttg gtactattcc ttgtgactaa agataggccg gaagaacctt
     5761 cgaccccatg acatcgcttg ccaaagcagt cggtcccctc gacggtcgcg gcaatttgcc
     5821 gctgtaccag gagctgcagc gggcgctgcg caaagccatt gacagccgca ttctcggccc
     5881 cgacgacgcg ctgccctcgg agcgcgatct ggccgccgat ttcgcggtgt cgcgcatcac
     5941 cgtgcgcaag gcactcgacg gactggtcgc cgacggcctg ttggtgcgcc ggcaaggcgc
     6001 cggtaatttc gtatcggcgc gcgtcgacaa gaactttgcg atgctgacct cgttctccga
     6061 ggacatgcgc gcgcgcggcc gcacgccgcg cagcgtgtgg ctgaagcgcg cggcggggac
     6121 cgtcacgccg gaggaggcgc tggccctgcg cttgagtccg ggcacgccgg tgtttcgctt
     6181 caatcgcatt cgctacgccg acgatgcgcc gatggccttg gaatacgcga ccatcgtcgc
     6241 gaccgcgttg ccctcgctcg aagcggtcga cacctcgctg tatgaagcct tggagcgcgc
     6301 cgggcagcgc ccggtgcgcg ccttgcaacg cctgcgggcc gtgcttctga ccggcgatca
     6361 ggcggaactg ctcaaggcca agccgggcga tgccggcctg caagtcgaac gcttgggttc
     6421 cttgcccgac ggtcgggcga tcgaattttc gcaatccttc tatcgaggcg atacctacga
     6481 tttcgtcgcc gagctgagcg cctcgacgtg acccgcgtgg acgcagccgt cgaagacacg
     6541 cggatgtttc aggaggccgc gagcgcctcc gatgccgtgc gcgaacaatt gcgctgcgac
     6601 ggtgccgcga tcgcggccat cggtgcgcaa ttgcgccgtc tcgcgccgcg cgccgtggtc
     6661 acctgcgcac gcggcagctc ggatcacgcg gcgacctacg ccaagtactt gatcgagacg
     6721 cgcgccggcg tgttgaccgc ctcggccgcg ccgtccgtga gctctgttta cggcgtttcg
     6781 caggatctct ccggctgcct gttcatcgca ctctcacagt cgggacgcag tcccgatttg
     6841 ctggcgtcgg tggctgcggc caaagcggcg ggcgcgacga ttctcgcgct gtgcaacagt
     6901 tccgatgcgc cgctcgtcgc cactgcagac tgggtgatcg agctgcgtgc cggcgccgag
     6961 accagcatcg cggccacgaa gtcctatctt gcgacgctgg ccgcgttgct gcggttggtg
     7021 gcggcgtgga cccgcaatgc cgccctcgag gcgaacatcg caacgctgcc gcgcttgatg
     7081 gatcagagtt gggcgctcga ctggtcggcg gcgctaccgc cgctcgagtc ggccgcgcat
     7141 ctgttcgtgg tgggccgtgg tcttggcctg ggcgccgctc aggagaccgc gctcaagtgc
     7201 aaggaaacgt gcggcttgca tgccgaagca ttcagcagcg cagaattgcg ccatggtccc
     7261 tacgccttgc tgggtccggg atttcccgcc ttgctgttca cgcaacgcga tgcgacgcag
     7321 gccggcgtcg aaaccttggc ggccgagctc gtgcggcgcg gcgtgccgct gctcatggcc
     7381 ggcgcgcggg tggcgggtgc catcacgctg cccacgctcg acagcagcgc cgagatcgct
     7441 cctctgctgt tggtgcaaag tgcatatcgg atgattgcga cgctcgcgct gcgccgtgga
     7501 ttcgatcccg accatccggc gcatttacgc aaggtcacgg agacggttta atgaggagcg
     7561 cactcgtcaa cggccggttg ttgaccggta agggaatcgt gagcggccgg acgctgctta
     7621 tttgcggagc acgcatcgaa gcgttggtgg atcccaagga tcggcgctgc cgcgacgccg
     7681 agcgggtcga tttgcagggt cagctgctgt tgcccggatt catcgatgtg caggtgaacg
     7741 gcggcggcgg cgtgctgttc aacgatgacc cgagccgcga atcgatcggc gccatcggcg
     7801 cggcgcaccg gcgtttcggc accacgggct tcctgccgac cctgatcagc gacgatctcg
     7861 acacgatcgg gcgggcgatc gacgccgtgc aatccgcgct cgacgccggc acccccggcg
     7921 ttctcggtat tcacatcgag ggaccttttc tcaatgccac gcgccgcggc gtccatgacg
     7981 ccaagcattt gcggctgctc gatgccgact caatagcgca gctgagccgc ctaagaggcg
     8041 ggcgcacggt gctcaccttg gcaccggaaa tgacgacgcc cggcgtcatc gcgcaactga
     8101 gcgcacgcgg gatcttggtg tccgcaggac acagcgacgc caactttgcc gaaaccatgg
     8161 cggccatcgc ccatggactc aagggcttca cccatttgtt caacgccatg gcgcgactcg
     8221 agccccgcga tccggggatc gtcggtgccg cgctgtacga tgaaaatacc tggtgcggaa
     8281 tcatcgtcga cggacaccac gtcgatccca tcatgctgaa gcttgcgctg cgctgtaagc
     8341 gccacgatcg cttcatgttg gtcaccgacg gcatgccggc cgtgggctcg agcgaaccca
     8401 cgttcgtatt gcagggccgc accattcggg tcgtcgacgg catctgccgc gacgagaacg
     8461 gcacactggc gggcaccgcg ctcgacatgg cggccgcggt gcgcaatgcc gtctccctgc
     8521 tggggctcga catcgtcgag gccgcgcgca tggcgagcga gtacccggcc gaatttctcg
     8581 gactgggcgc ggaactcgga cgcatcgagc ccggataccg ggcgaacctc gtgcagctgg
     8641 acgatgagtt cagagtgcaa aagacctgga tcgagggcct cgccgtctag ctcacggatg
     8701 cggggccggc ttcgccgctt tgggcgcctt cgactgcagc gccttcagcg tgtcgagcaa
     8761 gctaatgctg ggatgttcgc cacaataggc gagcagcgag gtctcgaagg cgcccaccgc
     8821 atccgcattg aaaagatggc tgccggatgt cgcgacggag aaaccgtaaa gccacattgc
     8881 gatggtcgtc gcgctctccg gatcgctgcg tctgaactcg gcatgccgcg ctgcgaatga
     8941 ggagcactcg accgcgctca aatccatcgg gttcttcgcc tcgggcttta cgatattcaa
     9001 agcatccaac atgctctcgg cgggattgct cttgcattcg ttgtcgagcg cgaagccgaa
     9061 cggcgccaat gcatccgggt acatgacgtg cgctccggat ttggcgacgg aatagccgaa
     9121 caaccacatc accgtattga tggagtccgc ggtcgggttg gtggcggccg cgggcaagac
     9181 ctcattctcg tatttgccgc agctcagcgt cgccagattc agcgtcccgg cgcggcccgc
     9241 gcagggcagc agaccgagga cgaacgcgag cgcgacgacg gctttcactt gctgagggac
     9301 tgcacattcg cgcgtgcggc gagacgcagc aggtcgtcga agtcatgcat cttatcgtcc
     9361 aagagattca gcttgcgggc gatcccctga atttccgatt cggcccgacg gttgacttca
     9421 aaatcgagtt cgctgcgcaa ccgatcgcgc gtgttctggc gattctggct catcatgatc
     9481 accggcgcct ggatggcggc cagcatcgag aggaacaggt tgagcaagat gaaggggtag
     9541 gggtcccagg cgctcttgcc gagcacgatg ttgatcagcg tgtagacggt gagcacgatc
     9601 ccgaacaacg aaataaaggt ccaggatccg ccgaatccgg cgaccgtgtc cgcaatgcgt
     9661 tcaccgaacg tcgcctcctt ctcgatgatg tcgttcgggt tgcggctggc acgcagcctc
     9721 gcgagctctt gcgcggcgtg gaattggcgt ccgagcacgg tcagaagatc cattcccgcg
     9781 tgcggctttc gctgcagcaa caccgcgatg tcatcgcggt cgacttcgat gcaactcgtg
     9841 tcctcgaggg caattgcgtt ggtgcgatga ggcgtctgct cgagcatcga ggcgaatcca
     9901 aagaactcac ccacctgggg ttcgtcgacg accacttcct gcccgtcatc gtccacgatg
     9961 cagacgcgca ccttgccgga gatcatcacg tacgcctggc cgccgggcgc gcccatctta
    10021 taaatccgtt ctcgcgatgc aaaggtcttc agttcaacct gcccggcgag caccgcggtc
    10081 tcatcgtcgt ccagcagggc gaagagcggc acactcttca gcaattccgg tttgcaagtc
    10141 atggcaagtc tcccgtgcag cctgatgcgg cgcaatcatg gcacaagaaa tttggcagtt
    10201 gcatgactat tgggagcgtg atggtcggcc gtggcatgac gctacgggtc ggcgcggcac
    10261 atcgagcgca atcgcgcaag gcctgccatg ccggcacgtt gcaacagcat gatggtcttg
    10321 ccgtcctgaa tcgccccgct gtcgatcatc tgcagcgccg tcgccaacgg cagctcgagg
    10381 acttcgatat cctcgccttc gcccgcatcg ccgccgccat cgctcgtgcg ggcttcatcc
    10441 cggtattcgg ccacgaagaa gtgcagcttc tcggtgaccg aaccggggct catgtaggcc
    10501 tcgaacactt tgtgcggcga gcttacgata tagccggttt cctcctgagc ctccttgcga
    10561 atgcaggtga gcggatcatc gccatcgagc aaaccggcgc acgcttcgat caacaagccg
    10621 tcggccgagc cgttgacgta ggtggggaag cgaaactgcc ggatcagcac caccgaatcg
    10681 cgttcgacgt tgaacaacaa gcacaccgcg ccattgccgc ggtcgtaggt ctcgcgcact
    10741 tgctcctgcc ggcggccgtc acgacgcaga tactcgaagc gggtcttctt gagaaggtac
    10801 cagtcatcgg acagcacccg ctcctcgatg atgcgcacgc gcgcggcggg cgcgggttct
    10861 gccggcgtcg aatgcgggtt gctggaatgt ggcatcttcg tgtaatatcg tgcagattcg
    10921 tgcaatcata acatgctgac cgcgcaacgc aagaagttgc tcctggctcg actcgccgca
    10981 gatggacaaa tcatcgcggt cgagctggcg cgcgagctcg gcacctcgga agacacgatt
    11041 cgccgcgacc tgcgggaact ggcgaaggcg ggcaagctgc agcgcgtgca cggcggcgcg
    11101 ctgcccgcgt ccgcggccgt gggcgacttg cacgagcgcg agcaggtgtc gccgcaggag
    11161 aagatcgcgc tgggccgtct cggtgccgcg atgatccgac cgggccaggt cgtcatcctg
    11221 gacggcggca cgacggcgct gcaagtggcg cgtcacctgg atccggagtt acgcgccacc
    11281 ctcgttacgc acagccccac cgtggcggtg gagttggccg ctcaccccgc aatcgagatc
    11341 atcgtgctcg gcggccggct gttccggcat tccatggtga gcgtcggcgc ggcgctgatc
    11401 gaagccgccg cgcaactgcg cgcggatctg tatttcatgg gagtgaccgg ggtccatccg
    11461 gaggcgggac tcagcacggg cgatgccgag gaggcggcgg tgaagcgcgc gctgcacgag
    11521 cgcgccgccg aaacgatcgt gctcgcctcg agtgaaaaga taatggccgc atccgccttt
    11581 gtggtcgcgc cgctaaagtc gttgagcctg ctggtggtgc cggccaaaac ccccgagcga
    11641 acgctgcgcg cgctgcgcgc gggcgggtcc aaggttcgac gggcgccgtg atgacgcgtg
    11701 ccggttgcca aggccgcgag tatccgacac catcgacaac ggtggcaaac acacacaaga
    11761 agttctcgat gcgcaccgtg gtcgcggcga gcgccatcgg caccaccatc gagtggtatg
    11821 actttctcat ctactcgacc gcggcatccc tggtcttcaa caaactcttc ttccccaccg
    11881 gcgatccatt gacaggcaag ctgctcgcga tcgcgacgat cggtgtggga ttcttcgtgc
    11941 ggccgttggg tgccatcgtg ctgagtcatt tcggcgaccg ggtgggacgc aagtccatgt
    12001 tgatcgtgac gctggtgacc atgggcgtcg gcacgatgct gatcgggttg ttgccgacct
    12061 atcaaagcat cggggtcgcg gcgccgatgt tgctggtcgc atgccgcgtg gcgcaaggca
    12121 ttgcagtcgg cggtgaatgg ggcggcgccg tgttgatggt catcgagcat tcgccgccga
    12181 atcgccgcgg cttctatgga agcatcgtgc aaatcggctt cccgctggga atggccatcg
    12241 gcaccgcctc gttcttcgcg ctggcgcggc tcgatgaggc cgagttcatg agttggggtt
    12301 ggcggctgcc gttcctggca agtgcgctgc tggtcgcggt cgggactttc attcgactgc
    12361 gcatcgacga gaccccggac ttcaaacgca atgtccgcga cgggcatatt gcccgcttcc
    12421 ccgttctcga cactctgcgc cgacacccga aagacctgct catcgggttg ggcgcgcgga
    12481 tcacggaagt ttcctggatc tatgtgatca cgattttcgg cttgagctat gcggtgacga
    12541 acatcggtct gccgcgcggg ctcgtgctcg gagcgattgc cgcgggagcc gccgtcgagc
    12601 tgatcacgat ccccgtatgc ggcgcgcttg cggatcggtt cggacgccgt ccgatctata
    12661 tgctcgggtg tctggccgca atcgccttgt catttccgat tttctgggcg atccaaacgc
    12721 gcgacccgtt gacggtggtg ctcggtttcg tgatcggcat gtcagtgggt cacggcatca
    12781 tgtatggcgt gcaggccagt ttcttgagcg aaatgtttcc cgccaatctg cgctacagcg
    12841 gtgcgtcctt gggctaccaa ctggctgcac cgatcggcgg cggactgata cccgtgatcg
    12901 ccgcagcaat cgtcgggcgc aatcacggcg ccacctggcc ggtctcgacg ctcatgatcg
    12961 ccctggccct ggttaccatg ctggccgtcg tgtatgcgaa agaaaccgcc ccggcgctcg
    13021 caacaacccg ggggactcac gtgcgcgggt gaattcggct tccgtccgga cgggttgagc
    13081 tcagaatttc gcgttccgct ggcggcagaa gccgtccgtc cagcgagagc tggcctgcgt
    13141 gaaaaatctg ctctccgccc cccggcaacg gtgtgccgcc gtgagcccag gcaccgcccg
    13201 gattctgcgg ggccgcgatg ccctgcagga caatcgagcg cgcggggacg gcttgctctt
    13261 cgatcgtgtc cattttgttc catccgggca aaaccgcgct cgcgctgcga gcggatgtcc
    13321 tggccaagta cgccccctga tcgtaggctg tcagccggcg cccggtctgc gggtcacgaa
    13381 catgctctgt ctggcctttt tcggcgatgc tatccgatcc gcgaagccac ctttcggtgc
    13441 tcccggtgac acgcaaggcg ggccggccga gaaaattccg gatttgtttc tcgagcgttt
    13501 gcaggaataa cagcagttgg ctcgacgcat cgggggagcg cgcactcgcg agccgagtga
    13561 gaattccgtc gggatcctgc agcagcgttt gcagccgctc gatcgaggcg gagaactcag
    13621 agcgttcaat cggttgagtc ggtccgggcc tcgaactgcg ggtccgcgcc agcgtccctg
    13681 agtcgatcca attgcgcccc agccgcacga cacaatgcgg tcccgatact cgatcgctca
    13741 ttgccgcggc ctcgggggta aaagacgcat gtcatgacga cgatacggac tgacgtcgcc
    13801 cgccgcaata ctcgccgcaa ggcaataaaa cgcttgcgcg catgccttac ccggcaagta
    13861 actccgcgcc cactctaccc tgatgccgaa gttgaggtta ttctgcggcg cacttcacgg
    13921 aggcgccatg cgcaaagatt tgatcgagca cagaaagtgg ctgtctcgta ccgcggtcat
    13981 cggcaagttt cgcagtaccg agctcaaggc gctcgatgcc gcgctgctgg cctacgaagt
    14041 cagcgggtct gaaagcgatc tgaagctgat tcgcaccacc ttggcggcgt ggaagcaaaa
    14101 agaagtcaag tcgggccacc cgtgggaaaa atccgtgcgc aacaaggacg gagccttcaa
    14161 ggagctcacc gagcggttgg atgccgagcc tgtatccaat ttctataccc aggacctggc
    14221 caagacgcgc ctgggtgtcc tctacctgtt ttccggaatg gatgtggagt cgggcatgtt
    14281 cgaggtgatt ctggacggcg gcttgaactt cgcggggaat gtcgccggtc tcggcggcgc
    14341 cttgacggcc agtgccgacg gcgccacggc ggccgcggcg atcggcacca acatcggcat
    14401 cgcgcaggcc atcagttacg aggtgctgaa cgcgacgccc gcgtcaaata cggcgaacaa
    14461 ctcaaagctc tccggcattc tgggtccgat caagacgcag ctggaaacct acgccaaaca
    14521 aatctgggcg cacctgacct cgatgttcaa gaaagacgcc agcaaatcct ggatcgactt
    14581 cgccggcgac tggtggtcgc gcattaccgg ggtgatcaat acggtgctca accagatcgg
    14641 ggtgagtgcc gcccccttcg tcggcgcagc cttcgatatc gtgcagggct cggccagaac
    14701 cgtgaaggct tgctatgacc gatatgtgac atattctctc tccaagggcg tcgacatgaa
    14761 cgacggccac ccgagcgtgg tggtgaaatc catcgagcgc accatgaatc tggccatcgg
    14821 ttcgggtctg taccaggcgc tgaagggcgc ggtgacgacc ggcgtcaacg tcgtcggctc
    14881 cggggccggc gccatcctgt cgatggtcgt atccggctgc gagctcattg ccaaggtcgt
    14941 ctatcgcctg tgggagtcga gcaagatgaa ggccttcttc gaggactgca aggatcgcta
    15001 tactaaattc aagacggcca agaaagttgc ggatgacgac gtgacgcaca gcgggatcgc
    15061 cttcggggcg tggtaccgcg cggccgccta tacgcttccg tgtctgtccg cgctcgccct
    15121 gtgcagcggc ttgaccggcg acaagatgat gtacttgagt atgttcaagg accagaagga
    15181 tttgacggcg cccgcgcaaa ccatgaatcc catttcccaa tccgagttcg atcgtggggt
    15241 cgcctatgtc gaccacctga aggaagccgc gaaggactat ttgtccagct cgggttacga
    15301 gttttcatcc accgacaaag tggtcgacgg catgttcaag agcaagaatt ttttcgggaa
    15361 caagtaacgg cgggcatggg cgtgtcgaac acgcatcgtg ttttgacttc ggcaattaat
    15421 ggtttatcct aaatgacgct gaaattttcc ccacgcgata cctaaaaaac accgataggg
    15481 ctcgagactg ctcatggccg gcatggatat aaacgcgctg gttcagccga tcggagaggg
    15541 tgcgccatgc ggcatcgatt tagaagatac tcagcaatta gccgccatcg acgccttccg
    15601 ggtgtttggc caaggcacgc cgctcgctgt ggacaccgac tggcgggcca tcaatgcggc
    15661 ggcgctcgag gcgcttgggg tcagccacga tttccgcctg ctcgcccatg tgtcggcggc
    15721 gcaactgagg ctcaccggcc tgcagccctt catcgactct ctgtgcgtcg cggcccgctg
    15781 gctcgaggat tccttcgccg acgtttttcc gcggatcgac gacgatgcgg ttctgcgcaa
    15841 gaacgcgctg aattacttcg ccgaccgcat ggccatcatc gatgcgctgc ggcgggcgcc
    15901 gctggtcagc caccggcagc ttgggtccta cacgctgcgc catatggcga tcgctgcggg
    15961 gcagcagacg ccgagcgagg aggaagccgc cgcgccgccg accgaagact tgatcaacgc
    16021 cgccttcgaa gcctcgccgc aggaagacct ggtgcagctg gtggcgtcga tcgacaccgg
    16081 cctcgcggcg ctgaaggcca tcgaagcggc aatggtggcc gagcacgggg tgcaagcagc
    16141 gcccgacacc ggacccttgg ccgaggtgct gacgcgaatg cgcgagcccg tggccaaaca
    16201 cgtgcgtgcg cccatgatcg aaactgccgc cgaagcggtc gacggcgaaa ctgcggccgc
    16261 gggtgtgcca gggcagattc gaagccgcga ggacgccgtg cgcagcctcg acgcggtcgc
    16321 cgattttttc cgtcgcagcg agccgtcgag tccggttccg atgttcgtgg agcgcgccaa
    16381 acgactgatt tcaagagatt ttctcgaggt cctggcggaa ctcgcaccgg agtctctggc
    16441 ggcggtgaag catgttgggg gaatccgcga gccagagtaa cctgtaactg atcgttaacc
    16501 ttaatttgaa ggggatgtcc tgtggccaaa ccgagcagcc agaaattcat cgcgcgaaat
    16561 cgcgcgccac gtgtgcaaat cgaatacgac gtcgaactct atggcgccaa gaaaaaggtg
    16621 caggtgccct tcgtgatggg tgtgatgtcg gatctgtcgg gcgattcgaa ggagccgctg
    16681 ccgtcggtgg acgaacgcaa gttcctgcaa atcgacatcg acaatttcga cgaccgcatg
    16741 aaatcgatga aaccgcgcgc cgcgttcacc gtaccgaaca cgttgaccgg cgagggcaat
    16801 ctgcaggtcg atctgacctt cgagagcatg gacgacttct cgccggccgc ggtggcgcgc
    16861 aaagtggacg ctctcgccaa gctgctcgag gcccgctcgc agctctccaa cctcgtcacc
    16921 tacatggacg gcaaggcggg cgcggaggag atgatcgcca aagttctcaa cgaccccgcg
    16981 ctcctgaagt cgctcgccgc aaaggcgaag cctcaggacg gcggcaattc ttgatttagc
    17041 gagggagacg atcatggccg aagcagaaaa agtagccgca ccgcaagtcg cgagtctcga
    17101 ggcagatcag ttcgaagccc tcctcgccaa ggaattcaag ccaaagacgg atcgggcgcg
    17161 cgccgaagtc gaaggcgcgg tgcgcacgct cgcggagcag gcgctgcgcg agagcacggt
    17221 ggtatccgat gacgtgatcg gcacgatcaa ggcgatcatc gcggagatcg acaagaaact
    17281 ctcggatcag atgaatctga tcctgcacag cgagcgtttc cagcagattg agagcgcctg
    17341 gcgcggcttg aattacctgg tcagcaacac tgaaacggac gagatgctga agatccgcgt
    17401 gctcaatatt tcgaagaagg atctggcaaa gaccctgaag aagtacaagg gggtcgcctg
    17461 ggaccagagt ccgattttca agcgcctgta cgaacaggag tacgggcagc tcggcggcga
    17521 gccctatggc gccctggtcg gcgattacta cttcgaccat acgccgggcg atgtggaact
    17581 gctcgggcag atcgcgcaga cagccgccgc ggcccatgcg ccatttctcg ccgccgccgc
    17641 gccctcgctg atgggcatgg actcgtggca ggaactgtcc aacccgcggg atcttgccaa
    17701 gattttcagt gcgccggact atgcggcatt ccgctcgctg cgcgaatcgg acgactcgaa
    17761 atacattggt ctggccatgc cgcgtttcct gtcgcgcgtg ccgtacggcg ccaagacgag
    17821 tcccgtcgag gagttcgact tcgaggagga gaccggcgcc ggcgaccaga acaaatacac
    17881 ctggaccaac gcggcctatg cgatggccgt gaacgtcaac cgctccttca agcagtacgg
    17941 ctggtgcacg tcgatccgcg gcgtcgaatc gggcggtgcg gtcgagggac tgccggtgca
    18001 caccttcccg agcgatgacg gcggcgtcga catgacctgc cccaccgaga tcgccatcag
    18061 cgaccggcgc gagatggaac tggcgaactc cgggctcatg ccgttgattc accgcaagaa
    18121 cacggactca gccgccttca tcggtgccca gtcgctgcag aagccgaccg agtacgacga
    18181 ccccgacgcg actgcgaacg cacgcatcgc ggcgcgcctg ccgtacctgt tcgccagctg
    18241 ccgattcgcc cactacttga agtgcatggt gcgcgacaag gtcggctctt tcatggagcg
    18301 cgaggacctg gagcgctatc tgcattcctg gattcagaac tacgtgctcg gcgatccctc
    18361 gcgggccggc gacaccttgc gggcgcagaa gccgctggcc gcggccgaag tcaaagtgga
    18421 ggagatcgag ggtaatcccg ggttctatgc ggcgacgttc cacctccggc cgcactacca
    18481 gctggagggt gtcaacgtgt ccatgcggct cgtgtcgaag cttccatcgc agaagaaata
    18541 accgcgctcc gttagccgcg gccgattctc gaggccgcgt ccggaacgca tcgaagtaag
    18601 ggcgctttat acagaagaag gagtgatccg atggcgatgg acatgttttt gacgttcgat
    18661 gaaggggcca agatcaaagg cgagacgatc gacaagacct ataaagcaaa gcacggcatc
    18721 gatgtgctcg cctggagctg gggcatcagc aattcaggtt ccgcgcatgt gggtcagggt
    18781 gcgggttcgg gcaaggcgaa tttccaggat atcagcttca cgaagtacat cgatctggcg
    18841 agctgtgcgc tgatggatgc ctgttcattg ggcactcaca tcaaggaagc caacctgatc
    18901 gtgcgcaagt cgagcggcga caaaaaggaa ccgctcgagt acctgaccat gaaattggag
    18961 acggtgctgg tcagctctta ttcgacggga ggcagcggcg gggaggatcg tttgaccgag
    19021 aacgtctcgc tcaacttcgc caaagtgacc gtgctttaca agcagcaggc agcgaccggc
    19081 gtggccggtg agaaaggtaa tttcacttac gacatcccgg caaacgctca aacctgaatt
    19141 ttcctgtccc acatggcgcg gtccgggtgg tttgcccgga ccgtgcgatc ggcccatata
    19201 gaagaacgcc atggccactg cagaagaaag tttccgcgcc ggcgacctcg ccggttgttt
    19261 ggccctgctc caggccgagg ttcgccgccg gcccgaggat gtgcggcagc gcatattcct
    19321 ggtgcaggtg ctgatggtgc tgggcgagtg ggagcgcgcg ctgacccagc tcggggtgct
    19381 caaggaaatg gatgcgagcg cgctgccgat ggtgcattcc tatggcgcag ccatccagtg
    19441 cgagcgtctg cgagcggcgg tgttccgcgg cgagcgcagc ccgttgctgt tcggcgagcc
    19501 ggagccctgg atggccgaga tggtccaggc gctcggactg caggcacggg gccatgcgag
    19561 cgaggcggcc gcgctgcgca gccgtgcgct cgaggcggcg cccgcctcgg cgggcacgct
    19621 cgacggccag gcattcgagt ggctggcaga cgccgacccg cggctcggtc cgctgttcga
    19681 agtgctgctg aatgccaact actactgggt gccgatgcac cgggtggccg aaatccgcat
    19741 cgaggcgccg tccgatatcc gtgatctggt gtggctgccg gcggagttcg tgtgggcgaa
    19801 tggcggcgaa gcggtgggct tcattccgac acgctacccc gggtccgaat cgaacacgga
    19861 cgatgcgatt cgcctcgcgc gcaagaccga ctgggtgggc gagggcgacc aaagcgtcgg
    19921 tttcggacag cgcatcctcg cgagcgatgc cttcgaggtg ccgctgctcg agctgcgatc
    19981 ggtcaagttc gagcggccat cgacgtgacg ccgcccgccc gccggtcggg aagttgaatc
    20041 atggccgaac tggcattcga ggatcggctg cagccggcgc tgctcgaccg cctgtccgac
    20101 gatgcgcgct cggtgctggt ggtcgaattg gtgaccagcc gcgcgcggct cgccgagcac
    20161 ggcgcctcca tcgacgccgt cgagttcgcg ctgcggccct ttggcctgcg gcgctggcgg
    20221 gcggatcgag actcgcccga ggccggcgcc gaggacgcgc tggtgctgca gttctctggt
    20281 cccaccgacg gcgcaaccct gacgcgggtg cgtgcgacgc ccgtggccgc gacgccgcag
    20341 ccgctgtccc tgtcgcgcct cgcggacgtt gcctcgcgct cgatcccgaa cttgaatccc
    20401 gaacccgccg cgcagcgcgt ggtgtcgatg cgccagctgc gcgagtcggt gttgcgcgac
    20461 ctctcgtggc tgctcgccac ctcgagctat gattcggacc gcagtctcgc agcctggccc
    20521 gaggtggagc gctcggtgtt caactacggc ctgccgagca tcgccggaca cggcctcagc
    20581 agcttcgacg ccaacgccgc cgccgcgcgg ttgcaaaagg ccgtcgaagc attcgaaccg
    20641 cgcctcgccc acgtgcacgt catctcggag gtgcaagccg ggaacatgga tcgcaacgcg
    20701 ttggtgtttc gcgtcgaggc ggagctgtgg ggtcagccga cgccgcagcg gatgctgctg
    20761 cggacgcaga tcgacgttga atcggcggac gttatgatca ccgatctcga gtccggctga
    20821 tggatccacg gctgctcgac tattacaact cggaactgcg cttcctgcgc gaatccggcg
    20881 tcgagttcgc gcgcgcctac ccgcgcatcg ccgcacggct cggcatggag tcgctcgaag
    20941 tcgccgatcc ctacgtcgag cggctgatcg aggcgttcgc gtttctcgcg gcgcgcgtgc
    21001 aattgaagct cgacgcgcgc catccgcagt tcaccgaaca tctgctcggc gtggtgcacc
    21061 cgagtttctt gaacccggtt ccggcgtgcg gcgtggccga gtttctgccg gatttgaagg
    21121 aagcctcgct gcaggcggga gttacgatcc cgcgcggttc gatcatgcgc acggtgctcg
    21181 ccaagggcga acgtacggcg agcgtattca cctccgcgca tgccgtgacg ctatggccgc
    21241 tcgccatcag cgaagcgaag tatctgtccg ggtcgggctc gctgaccggc accggcatcg
    21301 cggtcggcag ccaggtcaag gcggcgatcc ggctgcgtct gcaggcaagc cccggggtga
    21361 agctcaatca actgccgatc gagcggctgc ggttctacat caaggccacc cccgacctcg
    21421 cggcgcggct gtacgagcag atcgcggcga atgtgaccgg ctattacgtg cgcgcgccgg
    21481 gcgaacgcag cgtgaagttc tgtccggcgc aggcggtgcg tgcggccggc ttcgaggagg
    21541 acgaggcgct gctgccgacg ccgcggcgcg gcttcgaggg ttatcgcctg ctgcaggagt
    21601 atttcgcgtt tccggagcga ttcctgttct tcgacatcga tgagctgcgc gccgggcttg
    21661 cggactgcgc cggcggcgcg gcagagatct atcttgcctt cgatcgcgtg cagccgggcc
    21721 tcgagaacgc ggtggaccat tcccagttcc gcctgaattg cacgccgatc gtcaatctat
    21781 tccgccattc cccggatcgc gtgcacgtgg acgccgccgt caccgagcag catctggtgc
    21841 cggatcgcaa ccggccgatg gatttcgagg tgtacgaggt gctgcgcgtg acgggcgtga
    21901 gtgccagcgg cgaggtgctc gaggagatcc atccgctgta tctgtcaagc caccagtcgg
    21961 gcatcgagcg ctcgcggccc tactactcgt tgcaacgccg gccgcggctg ctctcgacgc
    22021 gccagcagca atcgggaacc cgcaccaatt atgtgggcac cgaggtgttc gtctcgctcg
    22081 ccgatgcacg cggccgcccg gtcgatgccg agatcacgca cttggatgtg caggcgctgt
    22141 gcaccaaccg cgacctgccc atccgcagcg ccttcggcaa gggacgcacc gactttcagc
    22201 tcgaggccgc ggcgccgctc gagggcatcc gctgcatcgc cggtccctcg acgccacgca
    22261 cctcgccggc cttcggcgat gcggcctgga acatcatcag ccacctgtcc ctcaattatc
    22321 tgtcgctcat cgatgccgat ccgggtcagg gagcgctgat cttgcgcgaa ctgcttgcgc
    22381 tgtacgcgga tccgaaggat ccggcggccg cccgccagat cgaaggagtc acgcacgtcg
    22441 cctacgcacc cgtggtgcgc cgcatcccga tacccggacc gatgagcttt gcgcgcggtc
    22501 tggaaatgac gctcaccctc gatgatgccg cgttcgaggg cgtcggtatc ctgacgctcg
    22561 cctcggtgct cgaacggttc ttcgcacgct acgtatcgct caattccttc gccgagctca
    22621 aggtgcgctc ctcctcgcgc ggcgaagtga agcgctgggc ggtacgcacc ggcacgcgac
    22681 aactgttatg aacccgctcg atcggttgcg cgaacagccc tacggcttct cgctgttcgc
    22741 cgcactgcgc ctgatcgagc aagcgtatcc cgagcggccg cggctcggcg aggcgcggcg
    22801 gccgggcgac gatgcggtcc ggctggcgca accgccgcac ctgattttcg cgccctccga
    22861 cgtcatgggg ctcgggcaga ccgagtccgg aacggcgcgc ctcgagcaat acggcttcgg
    22921 cgtctttggg ccgaatggcg cgctgcccct gcacctgacc gaatacgcgt tcgagcgcgc
    22981 cgcgcatcgc ggcgacccgg gggtcgtcga tttcatcaac acgttccagc atcgcttcat
    23041 cgcgctgttc taccgggcgt gggcggacgg cgatcccagc gtcaacctcg atcgtccggc
    23101 ctcggaccga ttcgtacgct gcttgggtgc gctggtcggg ctcgcaccgc cgagctcgcg
    23161 cgccgctgcg ccgctcgcgg cgcaggctgc gctcgggcgc gccgcgctct tcggc
//
LOCUS       DASWZT010000481         4119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_95954, whole genome shotgun sequence.
ACCESSION   DASWZT010000481 DASWZT010000000
VERSION     DASWZT010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95954"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /locus_tag="VGE92_13875"
     CDS             complement(<1..1143)
                     /locus_tag="VGE92_13875"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY1314965.1"
                     /translation="MNAKLSPAPSDGELDAFWMPFTANRQFKANPRLLARAEGMYYWT
                     SDGREVLDGVAGLWCVNAGHGRREITEAVTRQLGTMEYAPAFQMGHPIAFELANRLAR
                     IAPPGLDRVFFTNSGSESVDTALKIAIAYHRARGAGQRTRLIGRERGYHGVGFGGMSV
                     GGMVNNRKFFGPAMLPGVDHLAHTLSIEHNAFSRGLPQWGAHLANELERLIALHDAST
                     IAAVIVEPIAGSAGVILPPIGYLKRLREICDKNDILLIFDEVITGFGRVGKPFASLAF
                     DVTPDLMTTAKGLTNGAVPMGAVFAHRKIYDAFMQGPENAIELFHGYTYSAHPVACAA
                     ALATLDVYSKEGLLTRAASLAAEWEDAVHSLRGLPHVLDVRNYGLIG"
     gene            1256..1804
                     /locus_tag="VGE92_13880"
     CDS             1256..1804
                     /locus_tag="VGE92_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010563061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY1314966.1"
                     /translation="MSVDVGTHLKAVRQMYGLSQRELAKRAGVTNGLISLIEQNRVSP
                     SVSSLKKVLDGIPMALADFFTLDLGGQPQVFFPRQELSDIGTASVELRLVGSRLAKRT
                     MSILHERYAANADTGEEMLTHAGEEGGVIVKGKIELTVGGETRILGPGDAYYFKSTLP
                     HRFRNIGREECEIVSASSPPTF"
     gene            1828..2697
                     /gene="aguB"
                     /locus_tag="VGE92_13885"
     CDS             1828..2697
                     /gene="aguB"
                     /locus_tag="VGE92_13885"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEY1314967.1"
                     /translation="MSTVTVAATQMACSWDREANIARADKLIREAASRGAQIILIQEL
                     FETPYFCKDHASRHLELAKPLEGHPAVEYFAGVARELGVVLPISLFERANNAFYNSVV
                     IIDADGSVLGSYRKSHIPEGPGYHEKFYFSPGDSGFKVFDTRYAKLGVAICWDQWFPE
                     AARCMALMGADMLLYPTAIGSEPQDASIDSSGHWQRTMQGHAAANIMPLVASNRIGTE
                     QGEKYTMTFYGSSFIASHTGEKVAEADRSSESVLTATFDLDEVRRYRQSWGVFRDRRP
                     DLYYPLLSLDGRG"
     gene            2684..>4119
                     /locus_tag="VGE92_13890"
     CDS             2684..>4119
                     /locus_tag="VGE92_13890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEY1314968.1"
                     /translation="MVAAESLPEDLVFVDLETTGGNSAIDRITEVGIIRVRDGELIDE
                     WSSLVNPECSIPAYIEAFTGISNEMVAAAPRFAAIAALVRQKLHGAVFVAHNARFDYS
                     FLRSEFHKSEISFSAPLLCTVKLSRRLFPGYVRHNLDAVMERNGLTCSARHRALGDAQ
                     VLCDFWFKLRRELPVARLAAAVHALLGAQAVPAHLAGFVDDLPEGPGAYRLFGDDNVL
                     LYVGKGPSLRARVCSHLAAGDAHPKERRLADQVRRIDWQASAGELGAMLREAQWIKNE
                     KPLYNRRARHNARSYTLCAGKTQPLEARAIDAVERADLSHCYGVFHTEKDARKALSDI
                     ARAHHLCQKVLGFEVGAGSCVAFQVGKCKGACVAKEPIVLHNMRLHMALAALKFKTWP
                     FPGRIALRERARYGSELHVIDHWAYLGTARSEEEVATLAAGAGCDAFDVDVYKILVRH
                     FARNPRLDWLDLRRSVAADTFDCRAAYNA"
BASE COUNT          802 a         1238 c         1292 g          787 t
ORIGIN      
        1 gccgatcaag ccgtaattgc gcacgtcgag cacatgcggc agaccacgca gcgaatgcac
       61 cgcgtcttcc cactcggccg cgagcgaggc ggcgcgtgtc agcaatccct ccttggaata
      121 gacatcgagc gtcgcgagtg cggcagcgca ggcgacggga tgggcggaat aggtatagcc
      181 gtgaaacagc tcgatggcat tttccggccc ctgcatgaag gcgtcgtaaa ttttccggtg
      241 cgcaaaaacg gcgcccatcg gcaccgcgcc attggtgagc cccttcgccg tggtcatgag
      301 gtcgggagtg acgtcgaatg ccaagcttgc gaagggtttg cccactcgtc cgaagccggt
      361 gatcacctcg tcgaaaatca agagaatgtc gttcttatcg cagatctccc gcaggcgctt
      421 aaggtatccg atcggcggca gaatcacgcc cgccgagcca gcgatcggtt cgacgatgac
      481 cgcggcaatg gtcgacgcat catgcaaagc gatcaagcgc tccaattcgt tggcgagatg
      541 cgcaccccat tgcggcaagc cgcgcgaaaa ggcgttgtgc tcgatcgaca gggtgtgcgc
      601 cagatggtca acgcccggca gcatcgcggg gccgaagaac ttgcggttgt tgaccatccc
      661 accgaccgac atgccgccga aacccacgcc gtgatagccc ctttctcggc cgatgaggcg
      721 tgtgcgctgg cccgcgccgc gcgctcggtg atacgcgatg gcaattttga gggccgtatc
      781 caccgactcc gagcctgagt tcgtgaagaa aacgcgatcc agcccgggcg gggcgatcct
      841 cgccagccga ttcgcaagtt caaatgcgat cggatgaccc atctgaaacg ccggggcata
      901 ttccatggtg ccgagctgcc gcgtgaccgc ttcggtgatt tctcgccggc cgtgaccggc
      961 attcacgcac caaaggcctg caactccgtc gagcacctcg cggccgtccg atgtccagta
     1021 atacatgccc tcggcgcggg cgaggaggcg cggattcgct ttgaattgcc ggttcgcggt
     1081 gaacggcatc caaaatgcgt cgagttcgcc atcggacggt gcgggggaaa gctttgcgtt
     1141 cattgcggat ccttgcggta cttttgccat cgagccgggc caatgataag gaaaatcaac
     1201 actcagctca atttttgcgt ataaaccctg ccaactgtct attataatag acgccatgag
     1261 cgttgatgtc ggtacccact tgaaagccgt gcgacagatg tacgggctgt cgcagcgcga
     1321 actggcgaaa cgcgccggcg tcaccaatgg tttgatttct ttgatcgagc agaatcgcgt
     1381 cagtccctcg gtgagttctt tgaagaaggt gctcgacggc attccgatgg cgctcgccga
     1441 ttttttcacg ctggatctcg gcggccaacc gcaggtgttt ttcccgcgac aggagctcag
     1501 cgacatcggg accgcgagcg tcgaactgcg gcttgtcggc agtcgcctcg ccaaacgcac
     1561 catgtcgata ctgcacgagc gctacgcggc aaatgcagat accggcgagg aaatgctcac
     1621 tcacgcgggc gaagagggcg gcgtgatcgt caaaggaaag atcgaactca ccgtcggcgg
     1681 cgagacgcgg atcttggggc ccggggatgc ctattacttc aagagcacgc tgccgcatcg
     1741 cttccgtaat atcgggcgcg aagaatgcga gatcgtgagc gcaagctcgc ccccaacctt
     1801 ctgagtgctt caggtagcat agccgcaatg agtaccgtca ccgtggccgc cacccaaatg
     1861 gcatgcagtt gggatcgcga ggcgaatatc gcgcgcgccg ataagctgat tcgtgaagcc
     1921 gcgagccgcg gcgcacagat aatcctgatc caagagttgt tcgagacgcc gtatttctgc
     1981 aaagatcatg cgtcgcggca tttggaattg gccaaacccc tggaaggcca tcctgcggtc
     2041 gagtatttcg ccggcgtggc gcgcgaactc ggcgtggtgc tgcccatcag tctgttcgag
     2101 cgcgccaaca acgcgtttta caattcggtg gtgatcatcg atgccgacgg gtcggtgctc
     2161 gggagctacc gaaagtcgca cattcccgaa ggtccggggt atcacgagaa gttctatttc
     2221 tcgcccggcg acagcggctt caaagtgttc gatactcgtt atgccaagct cggcgtggcg
     2281 atttgctggg accaatggtt tccggaagcg gcgcgctgca tggcgctcat gggcgccgac
     2341 atgctgctct atcccacggc gatcggctcg gaacctcagg atgcgagcat cgactcgagc
     2401 ggacattggc agcgcaccat gcaaggccat gccgcggcca acatcatgcc gttggttgca
     2461 tcgaatcgaa ttggcaccga acagggtgaa aaatacacca tgactttcta cggatcgtca
     2521 ttcatcgctt cgcacaccgg cgagaaagtc gccgaggcgg atcgcagcag cgaatcggtc
     2581 ttgaccgcca cgtttgattt ggacgaggta cgtcgctacc ggcagtcctg gggggtattt
     2641 cgcgaccgcc ggcccgattt gtattacccc ctgctgtcgc tcgatggtcg cggctgaaag
     2701 tcttcccgag gatctggtat tcgtcgactt ggagacgacg ggcggcaact cggcgatcga
     2761 ccgaatcacc gaggtcggca tcatccgcgt ccgggacggc gaactgatcg acgaatggag
     2821 ttcgctggtc aacccggaat gctcgattcc cgcctacatc gaggccttca ccgggatcag
     2881 caacgagatg gtggccgccg cgccgcggtt tgccgcgatc gctgcgctcg tgcggcagaa
     2941 actgcacggc gcggttttcg tcgcgcataa cgcgcgcttc gactactcgt tcctgcgcag
     3001 cgaatttcac aagtcggaaa tcagcttttc cgcgccgctg ctgtgtaccg tcaaactgtc
     3061 gcggcgcctg tttcccggct acgtgcgcca caatctcgac gccgtgatgg agcgcaacgg
     3121 acttacatgc agcgcgcggc atcgcgccct cggcgatgcg caggtgctgt gcgatttctg
     3181 gttcaagctg cggcgcgaat tgcccgtggc tcggctcgcc gcggcggtcc acgcgctgct
     3241 cggtgcgcaa gcagtgcccg cgcacttggc ggggttcgtc gacgacttac cggaaggtcc
     3301 gggcgcctat cgattgttcg gcgacgacaa tgtcttgctc tacgtcggca agggcccctc
     3361 gctgcgtgcg cgcgtctgca gtcatcttgc cgcgggcgat gcccatccca aggagcgccg
     3421 cctggccgat caagtgcggc gcatcgattg gcaggcgagt gcgggtgagc tcggggcgat
     3481 gctgcgcgag gcgcaatgga tcaagaacga aaagccgctc tacaaccggc gcgccaggca
     3541 caacgcgcgc tcctatacgt tgtgcgcggg caaaacccag ccgctcgagg cgcgggccat
     3601 cgacgcggtg gagcgcgccg atttaagcca ctgctacggg gtatttcata cggaaaaaga
     3661 tgcacgcaag gcgctcagcg acatcgcgcg tgcccaccat ttgtgccaga aggttttagg
     3721 tttcgaagtg ggtgccggct cgtgcgtggc ctttcaggtc ggaaagtgca agggcgcatg
     3781 cgtcgctaag gaacccatcg ttctgcacaa catgcgcctg cacatggcgc tggcagcgtt
     3841 gaaattcaag acctggccgt tccccgggcg gatcgcgctg cgtgagcgcg cgaggtacgg
     3901 cagcgaactg cacgtgatcg atcactgggc ctaccttgga acggcacgca gcgaggagga
     3961 agtggcgact ctggcggccg gcgcggggtg cgacgccttc gacgtcgacg tctacaagat
     4021 tctggtgcga cacttcgcgc gaaatccaag actcgattgg cttgatctgc gccgcagcgt
     4081 tgcggccgat acctttgatt gccgggcggc gtacaatgc
//
LOCUS       DASWZT010000482         2822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_90802, whole genome shotgun sequence.
ACCESSION   DASWZT010000482 DASWZT010000000
VERSION     DASWZT010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90802"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1338
                     /gene="ispG"
                     /locus_tag="VGE92_13895"
                     /gene_synonym="gcpE"
     CDS             115..1338
                     /gene="ispG"
                     /locus_tag="VGE92_13895"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEY1314969.1"
                     /translation="MMSRRTSLGVKVGAVTIGGKAPIVVQSMTNTDTADIDGTVAQVK
                     ALARAGSELVRITVNNDEAAAAVPHIRERLDALGIGTPLVGDFHFNGHKLLKAHPACA
                     QALSKLRINPGNVGRGSKRDPQFAEMIETACKYEKPVRIGVNWGSLDQDLLTRMMDEN
                     SRLATPLAAIDVMREAIVVSALDSAKRAESLGLQPDKIVISAKVSGVQDLIAVYRNLA
                     ARCSYPLHLGLTEAGMGSKGIVASTAAMGVLLQQGIGDTIRVSLTPEPGGDRTQEVIV
                     AQEILQTMGLRAFTPMVIACPGCGRTTSTYFQELAQKIQNHIRHRMPEWRKRHTGVED
                     MSVAVMGCVVNGPGESKHANIGISLPGTGETPVAPVYEDGAKTVTLKGDRIAEDFQEL
                     VERYIERTYAKTGTS"
     gene            1335..1664
                     /locus_tag="VGE92_13900"
     CDS             1335..1664
                     /locus_tag="VGE92_13900"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002019.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY1314970.1"
                     /translation="MSGLSAKKCVPCEGGVAPMKPADVKRMRKHLHADWRVAKDSKSL
                     QRSLKFKDFYRTMSFVNALAHIANTEDHHPDLKLGYNYCDVTFSTHSIGGLSENDFIC
                     AAKLDRL"
     gene            complement(1633..>2822)
                     /locus_tag="VGE92_13905"
     CDS             complement(1633..>2822)
                     /locus_tag="VGE92_13905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY1314971.1"
                     /translation="ASLLIARRLAAPGRVAIVADRFSTAVPPDADWDLRVFALSRASQ
                     RLLEACGIWQSLPAGRKFAYERMCVWDAAGEPQGLSSLKFDCAELGEPNLGFIVDGRH
                     LLWQSLQAARAAGAVLIDAGLASIVAGDADIAARLNDGRTLRSQLLIAADGQESKSRT
                     LLGIETAGHAYHQDALVAHVRTAKPHDNTAWQRFLPGGPLALLPLPEGRSSIVWSVPR
                     AEAARLSALDDASFSAALTAASGNILGECTLASGRARYPLKLQYASQYARPRAVLLGD
                     AAHAVHPLAGLGLNLGLLDCAALAEVFEDARGAGHFGEYRILRRYERWRRSENLLAAG
                     ALDGLERLFSNAQPLRTALRAAGLAAVGRLPLVKRNLAQRALGLAGDVPKMLKADRAW
                     PHK"
BASE COUNT          538 a          921 c          922 g          441 t
ORIGIN      
        1 cctcgggttt ttctggattg ccttcgatca caacagccag gcctggcacg acaaaatcgc
       61 gggcacggtg gtggtgcgcg tggccaaggg cactcccctg gtataattgc gggcatgatg
      121 tcccgtagaa cgtccctagg cgtaaaagta ggtgccgtga ccatcggcgg caaggccccg
      181 atcgtcgtgc agtcgatgac caataccgat accgccgaca tcgacggcac ggtcgcgcaa
      241 gtcaaagcgc tggcccgggc gggttcggaa ttggtgcgga tcacggtcaa caatgatgag
      301 gccgccgccg cggtgccgca tatccgcgag cgtttggacg cgcttggcat cggcactccg
      361 ctggtcggcg atttccactt caacggccac aagctgctca aagcgcatcc cgcatgcgcc
      421 caggcgctgt cgaaactgcg catcaatccg ggcaatgtcg ggcgcggcag taaacgcgac
      481 ccgcaattcg cggaaatgat cgagacggcg tgcaaatacg aaaaaccggt gcgcatcggc
      541 gtcaattggg ggagcctgga ccaggatttg ctcacccgca tgatggatga gaactcccgg
      601 ttggcgacgc cgctcgcggc catcgacgtg atgcgcgagg cgatcgtggt gtccgctttg
      661 gacagtgcca agcgcgcgga gagcctgggg ttgcagcccg acaagatcgt gatcagcgcc
      721 aaggtcagcg gcgtgcagga cttgatcgcc gtgtaccgca atctggccgc gcgatgttct
      781 tatccgctgc acttgggctt gaccgaagcc ggcatgggca gcaagggcat cgtggcctcg
      841 accgccgcga tgggtgtcct gctgcagcaa ggcatcggcg atacgattcg cgtgtcattg
      901 acgcccgagc ccggcggcga ccgcactcaa gaggtgatcg tcgcccagga gatcctgcag
      961 accatgggct tgcgggcttt cacgcccatg gtcatcgcct gccctggttg cggccgcacc
     1021 accagcacct acttccagga actcgcgcag aagatccaaa accacattcg tcaccgcatg
     1081 cccgaatggc gcaagcgtca cacgggcgtc gaggacatgt cggtcgcggt gatgggctgc
     1141 gtcgtgaacg gtccgggcga gagcaagcac gcgaacatcg gcatcagtct gcccggcacc
     1201 ggcgagaccc cggtcgcccc ggtttacgag gatggtgcca agaccgtgac gctcaaaggg
     1261 gaccggattg ccgaggactt ccaggaactg gtcgagcgct acatcgagcg cacctacgcc
     1321 aagaccggaa cctcatgagc ggactgtccg cgaagaaatg cgttccttgc gagggcggcg
     1381 tcgcgccgat gaaaccggcc gacgtgaaac ggatgcgcaa gcacctgcac gcggactggc
     1441 gggtcgctaa ggactcgaaa agcctgcagc gctcgctcaa gttcaaggat ttctaccgca
     1501 ccatgagctt cgtcaacgcg ctcgcgcata ttgccaatac cgaagaccat caccccgatc
     1561 tgaagctcgg ctacaactac tgcgacgtca ccttctcgac gcattccatc ggcggcctgt
     1621 cggagaacga ttttatttgt gcggccaagc tcgatcggct ttgagcatct tggggacatc
     1681 gccggcgagg ccgagggcgc gctgcgccag gttgcgcttg accaggggca gccggcccac
     1741 ggcggcgagg cctgcggcgc gcagcgccgt gcgcagcggc tgcgcgttcg agaacagccg
     1801 ctcgagccca tcgagtgcgc cggcagccag caggttttcg ctgcgccgcc agcgctcata
     1861 gcggcgcagg atccggtact cgccgaaatg gcctgcgccg cgtgcgtcct cgaacacttc
     1921 ggcgagcgcc gcgcagtcga gcaaccccaa attgaggccg agaccggcca gcgggtggac
     1981 cgcatgcgcg gcgtcgccga gcaacacggc gcgcggtcga gcgtactgcg aggcgtactg
     2041 caacttgagc gggtagcggg cgcgccctga cgcgagcgtg cactcgccga gaatattgcc
     2101 gctggccgcc gtgagcgcgg ccgagaagga cgcatcatcg agcgcgctca agcgcgctgc
     2161 ctccgcgcgc ggcacgctcc acacgatgga cgagcggccc tccggcaggg gcaagagcgc
     2221 aagcggaccg ccgggcagga atcgctgcca ggccgtattg tcgtgaggct tggccgtgcg
     2281 cacgtgcgcc accagcgcat cttgatggta cgcgtggccg gcggtttcta tcccgaggag
     2341 cgtccgggac ttcgactcct gtccatcggc cgcaatcaac agctggctgc gcagcgtgcg
     2401 gccatcgttc aggcgcgcgg caatatcggc gtcgccggcg acgatcgacg cgagtcccgc
     2461 atcgatgagc acggcgcccg ccgcccgcgc cgcttgcaag gactgccata gaagatgccg
     2521 cccatcgaca atgaatccta gattcggttc gcccaattcc gcgcagtcaa acttcagcga
     2581 acttaagcct tggggttcgc cggcggcgtc ccagacgcac atgcgctcgt acgcgaactt
     2641 gcgtcccgcc ggcagcgatt gccagatgcc gcaggcttcg agcagccgct gcgaggcgcg
     2701 gctcaacgca aaaacccgca ggtcccaatc ggcgtcgggc ggcaccgcgg tggaaaagcg
     2761 atcggcaacg atcgccacac gtcccggcgc ggccaaccgg cgggcaatca acagactcgc
     2821 ca
//
LOCUS       DASWZT010000483         2126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_8630, whole genome shotgun sequence.
ACCESSION   DASWZT010000483 DASWZT010000000
VERSION     DASWZT010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8630"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VGE92_13910"
     CDS             <1..362
                     /locus_tag="VGE92_13910"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEY1314972.1"
                     /translation="AQIGAALATLSGVGGDSPLVKLQRSLGLDRLSVASATAYTPTGP
                     ESTGAAIQAGRYISKRVYVEAKQSSTGASQLEADVDLTKHLKLQTRLGNGTSSVLGVT
                     PENDPGSSVGLLYQFEY"
     gene            420..2072
                     /locus_tag="VGE92_13915"
     CDS             420..2072
                     /locus_tag="VGE92_13915"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314973.1"
                     /translation="MTLRIRCVIGASVLAALLAGSWSPAAEHSSPSDTPDTKIALVPD
                     PSESPRFLHDESKVTPGSITIGGHVLGYQSEAGILVVHVKDPLDDDPPPLRDDKSTPP
                     PPQPPEAGMSYVAYFRGDKEDSHRPITFLYNGGPGSSTVWLHMGAFGPKRVVTADDTH
                     SPAAPYRIIDNEYTLLDASDLVFVDAPGTGFGHLRGTDKEKAFFGVDQDAHAFANFIV
                     EFLSRHNRWNSAKYLFGESYGTTRSAALANILENEKSLDLNGVILLSQVLNFDDGVDQ
                     PQFNPGVDLPYLLALPTYTATAWYHHKLPNQPAALEPLLREVEAFALGDYMQALAAGS
                     SLSAERKSEIAARLHNYTGLAADYIERANLRVNGGEFEKTLLGADITTGRLDTRFAGP
                     TIDPMSKEADYDPQSAAISSAYVSAFNDYVRGTLKFGDKKTYKPEFNVDKLWDMLHQP
                     PGSETKVAGPINVMPDLAVAMKQNPNLKVQLNGGYYDLATPYFAAVYELQQLPIQSSL
                     KSNIEMHFYTSGHMVYAHEPDLKALHGNVAAFIERTRNASGK"
BASE COUNT          441 a          641 c          645 g          399 t
ORIGIN      
        1 tggcgcaaat cggcgcggcg ctcgcgacgt tgagcggcgt gggcggcgac agtcctttgg
       61 tcaaattgca gaggagtttg ggtctcgacc gtctctcggt cgcctcggcg accgcctaca
      121 ccccgaccgg acccgagagt accggtgctg cgatccaggc ggggcgctac atctccaagc
      181 gcgtttacgt ggaggcaaaa cagagcagca ccggcgcgag ccagctcgaa gcggacgtgg
      241 atctgaccaa gcatcttaag ctgcagacgc ggttggggaa cggcaccagc tcggtgctcg
      301 gagtgacgcc ggaaaacgat ccgggcagca gcgtcggcct gttgtatcag ttcgaatatt
      361 agcgaagagc tcgcccaaca gtttagaatg ggcgattgcc tgtcggggga gactcaagaa
      421 tgactttacg gattcgatgc gtcatcggcg cgtcggtact cgctgcgttg ctcgccggct
      481 cctggtcgcc ggcggccgaa cactcatcgc cgagcgacac gccggacacc aagattgcgc
      541 tggttccgga tccgagcgaa tcgccgcgat tcttgcacga tgaatccaag gtgacgccgg
      601 ggtccataac gatcggcggt catgtgctcg gctatcaatc cgaggccggc attttggtgg
      661 tgcacgtcaa ggaccctttg gacgatgacc cgccgccatt gcgcgatgac aagagcacgc
      721 cgccgccgcc gcagccgccc gaggccggga tgtcgtatgt cgcatacttt cgcggcgata
      781 aagaggattc acaccgcccg atcacatttc tgtacaacgg cggccccggc tcctccacag
      841 tctggctgca catgggcgca ttcggaccca agcgagtcgt cacggctgac gacacgcaca
      901 gccccgcggc gccttaccgc atcatcgaca acgaatacac gttgcttgac gccagcgatc
      961 tggtgttcgt cgatgcaccc ggtaccggat tcggtcattt acggggcaca gacaaggaaa
     1021 aggcgttttt cggcgtggat caagatgcgc acgcgttcgc caatttcatt gtcgaattct
     1081 tgtcgcggca caaccgctgg aactcggcga agtacctgtt cggcgagagt tatgggacca
     1141 cgcgctcggc ggcgctcgcc aacattctgg agaacgaaaa gtcgctggat ctaaacggcg
     1201 tgattctgct gtcgcaggtg ctgaatttcg acgatggcgt ggatcagccg cagttcaacc
     1261 ccggcgtgga tttgccgtac ctgttggctc tgcccacgta caccgcgact gcctggtatc
     1321 accacaagct gccgaatcag cctgcggctc tcgagccatt gttgcgcgag gtcgaggcct
     1381 ttgcgttggg cgactacatg caggcgttgg ccgccgggtc cagcttgagt gccgagcgta
     1441 aatcggaaat cgcagcgcgg ctgcacaact ataccggcct cgccgcggat tacatcgagc
     1501 gtgccaattt gcgggtcaac ggcggagaat tcgaaaagac tctgttgggc gccgacatca
     1561 ccaccggcag gctcgacacc cggtttgcgg gaccgacaat tgatccgatg agcaaggaag
     1621 ccgactacga tccgcaatcg gccgccatct cctcggcgta tgtatcggcg ttcaacgatt
     1681 acgtgcgggg cacgctcaag ttcggcgaca agaagaccta taagccggag ttcaatgtcg
     1741 acaaactttg ggacatgctg catcaaccgc ccggctcgga aacaaaggtt gccggtccca
     1801 tcaacgtcat gccggacctg gcggtcgcga tgaagcagaa cccgaacctc aaggtgcagc
     1861 tgaacggcgg ctactacgat ttggcgacgc cgtacttcgc ggcggtgtac gaattgcagc
     1921 agttgccgat ccaaagctcg ctgaagagca atatcgagat gcatttttac acctccggcc
     1981 acatggtcta cgcccatgaa ccggacctca aggcgctaca tggcaacgtg gcggccttta
     2041 tcgagaggac gcgcaacgca tccggcaagt gatgcgttcc ctgcaggcac gcgccgccat
     2101 gtaaaatttt caagaccgct cgggcc
//
LOCUS       DASWZT010000484         1707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_66689, whole genome shotgun sequence.
ACCESSION   DASWZT010000484 DASWZT010000000
VERSION     DASWZT010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66689"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1305)
                     /locus_tag="VGE92_13920"
     CDS             complement(<1..1305)
                     /locus_tag="VGE92_13920"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1314974.1"
                     /translation="MSIWAFAVRRWQFTLVVFALLIALGIASFMSIARSEDPSFPFPA
                     ATITLVWPGANPADMERLIVKPLEDAVNTLDNVKKIQSSAFDGLAVVHTEFYYGSDPE
                     KKQDEVIREFNRIRSSLPPDIAQIDIRKDSPGNVNIVQMALVSNSASWRTLKEKSQNL
                     KDAFESVPGVRTAETWAFPEPEVRVAIDLLRLGRSGVGLGQVIQAISGQSANVPGGAV
                     EVGLRRYNLRTSGSYTSLEQIADTVVASVNGRLVKVRDIADVSWQTAEYAYTGRFDGR
                     RAVFVTANQKDNTNIFKVRDGIYAKLADFEKTLPADIKLERGFDQSHNVAARLDRLGE
                     DFAIAIGLVLLTLLPLGLRAAGVVMISIPLSLAIGVALLFMAGFSLNQLSIAGFVLAL
                     GLLVDDSIVVVENIARYVRSGYSRIEAAIEATDQIYLAVLGCT"
     gene            complement(1302..>1707)
                     /locus_tag="VGE92_13925"
     CDS             complement(1302..>1707)
                     /locus_tag="VGE92_13925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY1314975.1"
                     /translation="LEFDAWPGQRFSGRVSNIASAADPATGTFTVELQVEAAGARFVQ
                     GLVAKVAIGLQNSGSVKLIPLQALLEANDKEAGVFVLDPRTHVVQRLNIQIGRMAQGR
                     IEVLDGLAADAQVVTDGAAFLENGETVRVESK"
BASE COUNT          326 a          551 c          510 g          320 t
ORIGIN      
        1 ggtgcagccg agcaccgcta ggtagatttg atcggtagct tcgatcgccg cctcgattct
       61 cgagtagccg ctgcgcacgt agcgggcgat gttctccacc accacgatcg aatcgtcgac
      121 cagcagcccc aacgccaaca cgaagccggc gatggacaat tggttcaagc tgaagccggc
      181 catgaaaagc agcgccacgc cgatcgccag agacaatgga atcgaaatca tgactacgcc
      241 cgccgcccgt aatcccaagg gcagcagcgt cagcagcacc aggccgatgg caatcgcaaa
      301 atcctcgccg agccgatcga gccgggcggc gacattgtgc gactgatcga agccgcgctc
      361 gagtttgatg tccgcgggca gcgtcttttc gaaatcggca agcttggcgt agatgccgtc
      421 gcgcaccttg aagatgttgg tgttgtcttt ctggttggcg gtcacaaaca ccgcacgtct
      481 gccgtcgaag cgtccggtgt aggcatattc cgccgtctgc cacgacacat cggcgatgtc
      541 gcgcaccttc accaagcgcc cgttgaccga agcgaccacg gtatcggcga tttgctccaa
      601 ggacgtatag cttcccgagg tacgcaggtt gtagcgccgc aggcccacct cgaccgcgcc
      661 gcccggcaca ttggcactct ggccggaaat cgcctgaatc acctgaccca agccgacgcc
      721 gctgcgcccg aggcgcagaa ggtcgatcgc gacgcgaact tcgggctcgg gaaaggccca
      781 ggtttcggcc gtgcgcacgc cggggaccga ctcgaaggcg tccttcagat tctgcgattt
      841 ctctttcaac gtgcgccaac tggcgctgtt gctcaccaac gccatctgca cgatgttgac
      901 gttgccgggg ctgtccttgc ggatgtcgat ttgcgcaatg tccggcggca gactcgagcg
      961 gatgcggttg aattcacgaa tcacttcgtc ctgcttcttt tccggatcgc tgccgtagta
     1021 gaactcggta tgcaccaccg cgaggccgtc gaaggcggaa ctctggattt tcttgacatt
     1081 gtcgagcgtg ttcacggcat cttcgagcgg cttgacgatc aatcgctcca tgtcggccgg
     1141 gttggcgccc ggccatacca gcgtgatggt cgccgccggg aacgggaaag acggatcctc
     1201 ggaccgcgcg atgctcatga aactcgcgat gccgagagca atcagcaacg caaacaccac
     1261 cagcgtgaac tgccagcggc ggaccgcgaa tgcccagatg ctcatttgga ctcgacccgc
     1321 acggtctcgc cgttttcgag gaacgccgcg ccgtcggtca ccacttgcgc gtccgctgcg
     1381 agaccgtcca gcacttcgat gcggccttga gccatgcgcc caatctgaat attcagccgc
     1441 tgcaccacgt gggttcgtgg gtcgagtacg aacacgcccg cttccttgtc attggcctcg
     1501 agcagcgcct gcagcgggat gagcttgacg cttccggaat tttgcaagcc gatggcgacc
     1561 ttggcgacga gcccctgcac gaagcgtgca ccggcggcct cgacttgcag ctccacggtg
     1621 aacgtgcccg tggcaggatc ggccgccgac gcgatattgc tgacgcggcc gctgaatctt
     1681 tgtcccggcc acgcgtcgaa ctcgagt
//
LOCUS       DASWZT010000485         3324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_47523, whole genome shotgun sequence.
ACCESSION   DASWZT010000485 DASWZT010000000
VERSION     DASWZT010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47523"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1285
                     /locus_tag="VGE92_13930"
                     /pseudo
     CDS             134..1285
                     /locus_tag="VGE92_13930"
                     /inference="COORDINATES: protein motif:HMM:NF026240.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1346..1564
                     /gene="dcd"
                     /locus_tag="VGE92_13935"
                     /pseudo
     CDS             1346..1564
                     /gene="dcd"
                     /locus_tag="VGE92_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005889365.1"
                     /note="Catalyzes the formation of dUTP from dCTP in
                     thymidylate biosynthesis; frameshifted; incomplete;
                     partial in the middle of a contig; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
     gene            complement(1561..2268)
                     /locus_tag="VGE92_13940"
     CDS             complement(1561..2268)
                     /locus_tag="VGE92_13940"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEY1314976.1"
                     /translation="MSSRGVLAMALLVAPAIAAEPPAAGTGLAAFSAHYEADWKSINI
                     GTSDLELKQDGESGHFIYTWTITARGVFRLYRAEVTQKSWLSIEAEHARPEKYRAADG
                     SSTVSLDFDWGGGRARGTSENKPVDLELKPGTQDVMSIQVEVMLDLKSGNLPNTFQIL
                     DNDELKEFIYSQEGSARIRTALGELDTVIVSSRRTGNNRILRMWFAPSLGFIPVQAER
                     TRDGKLEFAMRIKSVKN"
     gene            complement(2265..2939)
                     /gene="purN"
                     /locus_tag="VGE92_13945"
     CDS             complement(2265..2939)
                     /gene="purN"
                     /locus_tag="VGE92_13945"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEY1314977.1"
                     /translation="MNNERSRASVVVLISGRGSNMRTLIERGAAPGAAYRVSHVFSDQ
                     PAAAGLAVARDLGISAQVLGAAQFQNRADYDRALAAAIEACSPHLIALAGFMRILSAE
                     FVEAFAGRILNIHPSLLPKYPGLHTHRRVLEARDAQHGATVHFVTEQLDAGPSIIQGQ
                     VVVKANDDEASLAARVQAQEHRIYPLAVQWFGEGRLRYAAGNAWLDGQLLREPVKLDA
                     PPAEPP"
     gene            complement(2936..>3324)
                     /locus_tag="VGE92_13950"
     CDS             complement(2936..>3324)
                     /locus_tag="VGE92_13950"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEY1314978.1"
                     /translation="LTLVDRLMAPTRIYVKPLLNLIAKLPVHGLAHITGGGLVENIPR
                     VVPDGLEVVLERKSWRRDALFDWLQRQGQVADAEMYRVFNCGIGMTVQLAASDAERAI
                     GILRDMGQEALLIGEVRAGSRGVVIA"
BASE COUNT          661 a         1031 c         1003 g          629 t
ORIGIN      
        1 gaggatcggg ttcaccccgg gttcgatcaa acaacagaaa cttgactctg aggtgctgcc
       61 gttgattcgc gcccagccgc ggcgccgcag caacttcgac acgttgcccg cggaggcctg
      121 gcccatgatc gggatggcag ctcgcgtggg cggtgtaacc ctggcgtcga tcaatgttgc
      181 ttgcgtaact gcagggcgtt cggtgcccct cgccagcgcc gttcaggtgg ctctcgcgac
      241 tggggattta tatgtcagtc cgctcatcga tgggcccgtg tgggatgtcg tcgaatcgat
      301 cgaaccggca ggtgaggagg aagtattcga tattagcgtg ccatcgctgc acaacttcgt
      361 tgccaacgga ctcattgtgc acaattcgac gtacgcccgc tgttttcgcg gtgatacccg
      421 cgttgcgctg gttgatggca cgacgcccac tctcgaggaa atggcgcgca gacacgactc
      481 cggcgaactc ttctggggct atagcatcgg agaaaacggt cgcctgatcg tgagcttgct
      541 cgatgcgcct cgcttcattg gcagagacgc cttgttggaa atatcgttgg ataacggcga
      601 gctcatacac gcgactccag atcaccaatt catgaggcgt gatggacgca tggtggcggc
      661 gcacgcactt cgaccgacgg attcgctcat gccactctac cgcgatctat ttcgcggcta
      721 tgaaatgata tatcagccaa tcgacggcta tctctatccg actcatcgtc tagccgacga
      781 gtggaacctc aggcacggga tatatcctga actgccgggc acccaccggc atcacctcga
      841 cttcgatcgc cgtaataatt ttccaacgaa cattcagcgg ctcgaagcat cggaacacat
      901 tcgccttcat aatggagaaa actacggcga gaatttcgac ccgcgtcagc acggggcggt
      961 gataagcgca agcctcgcaa gacgcgcgca agatccacag tggcgtgaat atttcagtca
     1021 tgttcaagcg cttcgggcca aacaattttg gcgcgatgac cgattcgctg gcattcgcaa
     1081 tcgcgtcatc gagcagcgtc gaaatccgac agacgcaacg cgtgaggccc gtacgaggcg
     1141 cactaagcag gccgaggtgg cgcgaagaat ccgcctacga gccgggcgtt tccccgatac
     1201 tattgcgaga ttccggggca cgcccgcgta tcgcaatcac aaagtcgtcg ccgtgcgcgg
     1261 gttacccggc gatcacgatg tgtactgcct gaccgcaccc gaggcgggca actttgcctt
     1321 ggagagcggg attttcgtac aaaattgcgg aattatagtc aacgtaacgc ctttggaacc
     1381 cgcgtgggag gggcacgtta ccttggaatt ctcgaatacc acgccgctgc cggccaagat
     1441 ctacgccaac gaaggcgtgg cgcaaatgct gttcttcgaa tccgacgaag tctgcgaaac
     1501 ttcgtaccgt gatcgcggcg gcaagtacca aggccaacgc ggcgttacgc tgccgaagac
     1561 ttagttcttg acgcttttga tgcgcatggc gaattccaat ttgccgtcgc gcgtgcgttc
     1621 ggcttgcacg ggtatgaagc cgagcgaggg tgcaaaccac atccgcagaa tgcggttgtt
     1681 gccggtgcgc cggctcgaaa cgatcacggt gtcgagctcg ccgagcgccg tcctgattcg
     1741 cgcgctgccc tcctgggaat agatgaattc cttcagctca tcgttgtcga ggatttgaaa
     1801 ggtgttcggc aggttaccgg acttcaggtc gagcatgact tcgacctgga tggacatgac
     1861 atcctgcgtc cccggtttga gctccaagtc gacgggcttg ttttccgagg tgccgcgggc
     1921 acgtccaccg ccccaatcga aatccaggct caccgtggat gagccatccg cggcgcggta
     1981 tttttccggg cgggcgtgct ccgcttcgat gctcagccag cttttctggg tgacctcggc
     2041 ccggtacagc cgaaacacgc cgcgcgcggt gatggtccac gtgtaaatga aatgaccgga
     2101 ctcgccatcc tgtttgagtt ccaggtcgct ggtgccgatg ttgatgcttt tccaatccgc
     2161 ctcgtagtga gccgaaaacg ccgccaagcc ggtgccggcg gcagggggct cggctgcgat
     2221 cgccggcgcc accagcagcg ccatcgccag gacgccccgc gaactcacgg gggctctgcg
     2281 ggcggcgcgt cgagcttgac cggctcgcga agcagctgcc cgtccagcca cgcattgccg
     2341 gccgcgtaac gcagtcttcc ctcgccaaac cattgcacgg cgagcggata gatgcggtgc
     2401 tcctgggcct gcactcgcgc cgccagactc gcctcgtcat cgtttgcttt gaccacgacc
     2461 tgcccctgga tgatcgacgg acctgcatcg agttgttcgg tcacaaaatg caccgttgcg
     2521 ccgtgctgcg cgtcgcgcgc ctcgagaacg cggcgatgcg tgtgcaggcc cgggtattta
     2581 ggcaacagcg acgggtggat gttcaaaatt cgaccggcaa aggcctcgac aaactccgcg
     2641 gacaagatcc gcatgaaacc ggccagcgca atcaagtggg gcgagcaggc ctcgatcgcc
     2701 gcagccaagg cgcgatcata gtccgcccgg ttctggaact gcgccgcgcc gagcacctgc
     2761 gccgagatgc cgagatcgcg cgcaactgca agccccgcgg cggcgggctg gtccgagaac
     2821 acgtgcgaca ctcgataggc ggcgccggga gcagcgccgc gctcgatcag cgtgcgcatg
     2881 ttgctgccgc gtccggatat caggacgacg accgacgccc gggatcgctc gttgttcaag
     2941 cgatgacgac accgcgagaa cccgcgcgaa cttcaccgat gagcagcgcc tcctggccca
     3001 tgtcgcgcaa aataccgatg gcgcgctcag cgtcgctcgc agccaactga accgtcatgc
     3061 cgatcccgca attgaacacg cgatacatct cggcatcggc aacctgacct tggcgctgca
     3121 gccagtcgaa cagcgcgtcg cggcgccagg acttgcgctc gagcaccact tccaaaccgt
     3181 cgggcacgac ccgcggaata ttctcgacga ggccgccgcc ggtaatgtgt gccaacccgt
     3241 ggaccggcag cttcgcaatg aggttcaaca acggcttcac gtagatgcgc gtcggggcca
     3301 tgagccgatc aaccagggtg aggc
//
LOCUS       DASWZT010000486        11389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_66696, whole genome shotgun sequence.
ACCESSION   DASWZT010000486 DASWZT010000000
VERSION     DASWZT010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11389
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66696"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..1446
                     /locus_tag="VGE92_13955"
     CDS             88..1446
                     /locus_tag="VGE92_13955"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY1314979.1"
                     /translation="MNNPVAYPDRHESTTTILKRAAVRCLVVHDDLELRLRLASLVRR
                     AMPKVDADCITCASFDSLPPERVAAYAALLLVVEFNLRDHSVDPLARLERTRRQAPHL
                     PIFAFARGGDERNAARTIKLGANDYWPVHAVKIGELTAVLQPLLEPPPAPPPPEPAAA
                     SSAPAADSRRQPQIAGYRLVKTIAESAAAAVYLARNDDLTQPVALKVQALKGRHGVSD
                     ADRQRFVRECEILSSLNHRSIADVLDYGVTEDYMYLALEYFPCGSLRDRLRNPVSEAD
                     AVNYGRQIGEALQVLHIAHVVHRDLKPSNLMLTDDNRVILIDFGSASMRFEASDLSRA
                     DLCTGTPYYVCPEQIDNCDPDARGDLYSLGVVLYEMLVGALPFVGSSLQEIFTAHRSA
                     PVPRLPQRVLRYQPIIDRLLAKSPAERYPSAGLFLEDLSAVSAPIRTSVVGVSDMGVS
                     GP"
     gene            1443..3650
                     /locus_tag="VGE92_13960"
     CDS             1443..3650
                     /locus_tag="VGE92_13960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1314980.1"
                     /translation="MNLRFTKLVAPLRTPAGLKVLVVVLGLSFVALVLVPGLELASVV
                     TERSTALNMLGEQQRHPTLIRASLEAVRERLGARGYFQDSLDQLRISNNKLDTALHEM
                     TVERPAGWFASLVNSGAAGTPIAGKHAAQLLDSWARERVVLNPVLAFNGVPYQDNETT
                     GTYLTDSGRDLERDVNAALRTSRHVLPVLDSEFTAIANELESANVRSSNQLRLIMLSG
                     VVIALALVAVVTVLLSARQRQENSLRRARQQTENILRTVKDGLFLIDKNLIIGSAYSS
                     ALESLFQRKDFEGLAFESLLKNIVSARTMATALKYVKILWSERTNEKLVKTINPLGEV
                     EVNLDGGHGKSDTRFLQFDFHRVRQDGEMTHVLVSVSDVTTRVDLTLELQASKTQAQD
                     QVDTLLGILQIDPGQLQSFLNDSNAAMKMINAVLREPSREEGVFRKKLDSLFRQVHSV
                     KGEAAALHLSSIENRAHAFEEDLKALRDKPALSGNDFVPLVIKLDDLLTHLQSVSDMV
                     SRLSRMQIAQHDAPHTITAVIEEEEKQAPATGEADSGLVATLQQLAARVARENNREVD
                     LLCTGFDAVPEEYRRAVKDIAIQGVRNAVVHGIELPAARVAAGKPKQGTVRLTFQDAG
                     EAGYKLIIEDDGQGLGIERIKEVALKKGIISAERAQSMDAKQVFGLLFKSGFSTVETA
                     TKDAGRGVGMNLIADVTNQLGGRVSLATSNGKFTRLTTTLPRSPKRAADAEAA"
     gene            3650..4042
                     /locus_tag="VGE92_13965"
     CDS             3650..4042
                     /locus_tag="VGE92_13965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1314981.1"
                     /translation="MPIEAVAKASLKLMIVDDSNIIRRRIERSQQIDRLEVVGAASNG
                     REAVELFRRTQPDVVTMDLTMPEMDGVECVQKLVAINPGVLILVVSALADKATAVDAI
                     EKGANGFLCKPFTDRQLNDALEELLSEA"
     gene            4042..4482
                     /locus_tag="VGE92_13970"
     CDS             4042..4482
                     /locus_tag="VGE92_13970"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HEY1314982.1"
                     /translation="MLKEAEIRTFIDGTTNYFEIAAQQAATVGSPYLVTDGNPGAYEY
                     TGVINISGARTGIVYFTAPRGMLTVLLMRMQETDTSDENIKDLVGEVANTISGNARRD
                     FGKNFHISVPVVHDTEKVAAPHSRSYVIPINWRTHSAKLVVCLE"
     gene            complement(4489..5910)
                     /locus_tag="VGE92_13975"
     CDS             complement(4489..5910)
                     /locus_tag="VGE92_13975"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEY1314983.1"
                     /translation="MHHDLDRRAALAGLLSAALIQTPVALALPTDPGVRHAATDNGTP
                     QPLAGIGTDEMNFFLDGMARFNFIEVVNGGNSQQGTGNGLGPRFNSNQCASCHTQPNV
                     GGSSPPQNPLSAVANADGATNTMPWFNAANGPIREARFVQTNGAPDGGVHNLFVVTGR
                     SDAGTCNIAQPNFLPAGNPLTGQGGNRNIVFRIPTPVFGAGLIEAIPDSAILANAQTN
                     AAAKSQVGVRGRANAILGGNVNSSANDGTITRFGWKAQNKSLLMFAGEAYNVEMGVSN
                     QLFPQERDETAACRTYPSTPEDTNNMPLTVTGNAMAVLSDIEAFANFMRLLAPPAPAA
                     SNASTLNGRAEFASTGCALCHTPSFNTGRAVSSGSSNNPSPALSNQPVNLFSDLLVHH
                     MGQGLADGITQGAAGPDEFRTAPLWGVGQRVFFLHDGRANDLLTAIQAHESPGSEANG
                     VIQRFNNLSAQHQQDILNFLRSL"
     gene            6126..7277
                     /locus_tag="VGE92_13980"
     CDS             6126..7277
                     /locus_tag="VGE92_13980"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEY1314984.1"
                     /translation="MLNRREVLSGAAAAAALGRWSQARSGTPASGEFSKSQYQRAIVI
                     DALGAVGENDPAAADDAPLTARGLEDARESGVTAVNFTINEPGNGPDRFKIAITNIAG
                     VEHEFTAHPEFLVKIQRAADIQAAKASKRIGIIYGCQDVTMLEGDINKLATFADLGLR
                     IVQPTYNRRNLLGDGCLEKADGGLSKMGYDLIAEINRLNLLLDLSHAGVRTIAEGIAA
                     SKAPMAITHTGCRALVDLPRNTPDTELKALADRGGVVGIYFMPFLRASGQPHAEDLIR
                     HIEHAVNLCGEEHVGLGTDGSISAVPLTDAYREWFRKSVEARQKAGISAPGESADVFT
                     LVPEYNDPLRFLHLANDLARRGWPGSRIEKLLGANFARLFADVWDAHRA"
     gene            complement(7284..7892)
                     /locus_tag="VGE92_13985"
     CDS             complement(7284..7892)
                     /locus_tag="VGE92_13985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1314985.1"
                     /translation="MLWYPPSIAPFAAIRHALVVPDLDAARAAGLGESAYFIGICPAS
                     LPERWRFVSHSRILQLFPTLDLGPAEEEAGTVLGEADRAAMQELTQIAFPDFFRARTA
                     ELGLYLGVFSGGKLVAMAGERLALDGLQEISGVCTHPEHAGQGHARRLTRALLSRQRR
                     RGVRSFLHVSEGNSAARSLYDSMGFKVRASLGLCKVERLRST"
     gene            complement(7998..8813)
                     /locus_tag="VGE92_13990"
     CDS             complement(7998..8813)
                     /locus_tag="VGE92_13990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY1314986.1"
                     /translation="MSTTNSLGTALITGASTGIGATYANRLARRGYDLILVARNKPNL
                     EKLAATLGAASAVRITVLPADLTVDADVQRVEQVLRDDAGITLLINNAGAATLGAFVK
                     ADIGRLSRDIQLNIVAPTRLTYAALPGLLKRNRGSIINIASVMSQMVQPGNSVYGGTK
                     AYLLHFSEVLALELAASAVRVQVVLPGATGTALWNGSGVELKDLPPDIVMNVDEMVDA
                     ALAGFDQGERVTLPSLPEAADWQRLVKAREDLQPNLSRKHSAPRYKIPLKAAV"
     gene            8938..9555
                     /locus_tag="VGE92_13995"
     CDS             8938..9555
                     /locus_tag="VGE92_13995"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY1314987.1"
                     /translation="MGHSKASKEQTHARLVAAAAARFKERGVDGISLADLMKDLKLTH
                     GGFYKHFDSRDELVTEALDLALKQSGASVRERLFGGDEPDLSRFVDFYLAEAHRDGRA
                     DGCAVAALAGDAPRKGAAVHAQFRAHIESNLANLVEALAPGGSPAERRASALMVLSAL
                     YGALMMARAVGDSPLSREILRTARKRIPALGGVRSLPEKKTRNPH"
     gene            9612..10571
                     /locus_tag="VGE92_14000"
     CDS             9612..10571
                     /locus_tag="VGE92_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314988.1"
                     /translation="MSLLSCLPLPAAAGDAAIGATVPAWLFPHNAPSLAADNTVSAKL
                     EHLKGSRLGYTRAQLTDLFFAPDWHPESHAAPPQIVLHGRAPGVYACGYCHLPTGQGR
                     PENAPLAALPAAYIVQQVLDMRTAARHSAWHGAAFVPVDLMRDVAANVSDAELAAAAG
                     YFAAQPLRGRVTVIERATIPRMRVAAWIYARDSRGGTEPLGQRLIEWAPDLSRHERRD
                     EDLRYTAFVPPGSVNRGREIAMHGLPGVQACAGCHGRRLQGTGLLPPLAGRSPTYLLR
                     QLVAFQTKDRAGLMAVPMQAVVANMQLPDLIAVAAYAASASAN"
     gene            complement(10568..>11389)
                     /locus_tag="VGE92_14005"
     CDS             complement(10568..>11389)
                     /locus_tag="VGE92_14005"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1314989.1"
                     /translation="GSRRRPMTLRPIAGIVLRQIYLLRGSPVRLFPMVTWVAIDIVLW
                     GFITRYLNTVSGSGVDFVPTLLGGVLLWDFCTRIMQGVTTAFLEDVWSRNFLNLFASP
                     LSIAEYIAGLVISSVATSSVGLLVMLLLATGVFHLEFFAMGLPLAAFLLVLFVFGISL
                     GIFGSAVVLRLGPASEWLLWPLPALLSPFAAVFYPLAVLPAWMRWVSYALPPAYVFEG
                     MRGLLAHRSFPAASLLQGALVAALELLLAAYFYARVYRHAVRTGLLARYSAESVS"
BASE COUNT         2117 a         3578 c         3734 g         1960 t
ORIGIN      
        1 ctaccaaccg gcgcagcgcg acggcaaccc gatcgccgtg agcacccgcc tacgaatcgc
       61 atttcgaatg tcgaagtaaa cgaacgaatg aataaccctg tcgcctaccc cgatcgacac
      121 gagtccacca cgacgatcct caagcgcgcc gcggtgcgtt gcctcgtggt gcacgatgat
      181 cttgagctgc gcttacggct ggcatcgctg gtgcgccgcg ccatgcccaa ggtcgatgcg
      241 gactgcatca cctgcgcgag tttcgattcg ctgccgcccg aacgcgtcgc ggcgtacgcc
      301 gccctgttgt tggtcgttga gttcaatctg cgcgatcata gcgtcgatcc gctcgcgcgg
      361 ctggagcgca cccgccgcca ggcgccgcat ctgccgatat tcgccttcgc gcgcggcggc
      421 gatgagcgta acgccgcgcg cacgatcaaa ttgggcgcca acgactattg gccggttcac
      481 gccgtcaaga tcggcgagct gaccgcggtg ttgcagccgc tcctcgagcc gccgcccgcc
      541 ccgccgcccc ctgaacccgc tgcagccagc tccgcgccgg cggccgattc gcggcgtcag
      601 ccgcaaattg ccggctaccg cctggtcaag acgatcgcgg aatcggccgc agcggccgtg
      661 tatctcgcgc gcaacgacga tctgacgcag ccggtggcgc tcaaagttca ggcgctgaaa
      721 ggccggcatg gggtgtccga tgccgatcgc cagcgcttcg tccgcgaatg cgaaatcctc
      781 tcctcgctca atcaccgctc gatcgccgac gtgctcgact acggcgttac cgaggactat
      841 atgtatctag ccctggaata ctttccgtgt gggagtctgc gcgatcggct gcgaaatccg
      901 gtgagcgagg ccgatgccgt gaattacggg cggcagatcg gcgaggcact gcaggttctg
      961 cacatcgcgc acgtcgtgca tcgcgatctc aagccctcga atctgatgtt gaccgacgac
     1021 aatcgcgtga tcctgatcga cttcggttcg gcgtccatgc gcttcgaggc cagcgatctg
     1081 tcgcgcgccg acctgtgcac cggcactcct tactacgtgt gcccggagca aatcgacaat
     1141 tgcgatccgg acgcgcgcgg cgatctgtac agcctcggcg tcgtgctcta cgagatgttg
     1201 gtgggtgcgc tgccgttcgt cggcagcagc ctgcaggaga ttttcacggc gcatcgctcg
     1261 gcgccggtgc cgcggctgcc gcagcgcgtg ctgcgctatc aaccgatcat cgatcgcctg
     1321 ttggcgaaaa gtcccgccga gcgctatccc tcggcgggcc tgtttctcga ggacttaagc
     1381 gcagtgagtg cgcccatccg cacgtcggtc gtcggagtca gtgatatggg agtgtctggt
     1441 ccatgaattt acgcttcacc aagttagtgg cgccgctgcg cacgccggcc ggcctcaagg
     1501 tgctggtggt ggtcctcggc ctgtccttcg tcgcattggt gctcgtgccg ggactggaac
     1561 tcgccagcgt ggtcacggag cgctcgacgg cgctcaatat gctcggcgag cagcagcgcc
     1621 atccaacgct gatccgcgcg tctctcgaag cggtgcgcga acgcctgggc gcgcgcggct
     1681 actttcaaga ctcgctcgat cagctgcgaa tctccaacaa caaactggat acggcgctgc
     1741 acgagatgac cgtcgagcgg ccggccggtt ggttcgcctc gctggtcaat tccggggcgg
     1801 ccggcacgcc gatcgccggc aagcacgcgg cccagctgct cgatagttgg gctcgggagc
     1861 gggtggtgtt gaatccggtg ctcgccttca atggagttcc ctatcaggac aacgagacca
     1921 ccggcactta tctcacggac agcggccgcg atttggagcg cgacgtgaac gcggctctgc
     1981 gcaccagccg ccacgtgttg ccggtgctcg acagcgagtt cacggcgatc gccaatgaac
     2041 tcgagtcggc caacgtgcgt tcgtcgaacc aactgcggct gatcatgctg tcgggcgtcg
     2101 tgattgcgct cgcgctggtg gccgtggtga cggtgttgct gagcgcgcgg caacgccagg
     2161 aaaatagttt gcggcgcgcg cggcagcaga ccgagaacat tttgcgcacg gtgaaagacg
     2221 gcttgttcct catcgacaag aacctcatca tcggttcggc ctattcgagc gcattggaaa
     2281 gcttgttcca gcgcaaagac ttcgagggcc tggcgttcga gagtctgctg aaaaacatcg
     2341 tgtcggcgcg caccatggca accgcgctga agtacgtcaa gattctctgg tcggagcgca
     2401 ccaacgaaaa gctggtcaag accatcaatc cgctcggcga ggtggaagtc aatctcgacg
     2461 gcggtcacgg caaatcggac acccgctttc tgcagttcga ttttcaccgc gtgcgccaag
     2521 acggcgagat gacccacgtg ctggtgtccg tctccgacgt caccacgcgg gtggatctga
     2581 ctctcgagct gcaggcgtcg aagacgcagg cgcaagatca ggtcgatacg ctgctcggca
     2641 ttctgcaaat cgatccgggg cagctgcaat ccttcctcaa tgactcgaat gccgccatga
     2701 agatgatcaa cgcggtgttg cgcgagccgt cgcgcgaaga gggcgtgttc cgcaagaagc
     2761 tcgattcgct gttccgtcag gtgcactcgg tcaagggcga agcggcggcg ctgcatttat
     2821 cctcgatcga aaatcgcgcc cacgccttcg aagaggactt gaaggcgctg cgcgacaagc
     2881 cggcgttgtc gggcaacgat ttcgtgccgc tggtcatcaa gctggacgat ctgttgacgc
     2941 atctgcagtc ggtcagcgac atggtgtcgc gcctgtcgcg catgcagatt gcgcagcacg
     3001 atgccccgca cacgatcacg gccgtgatcg aagaggaaga gaagcaggcg ccggcgacgg
     3061 gggaggccga ctccgggctg gtggcgacgc tgcagcaact ggcggcgcgg gttgcccgcg
     3121 aaaacaaccg cgaggtcgat ttgctctgca ccggcttcga cgcggtgccc gaggaatatc
     3181 ggcgcgcggt gaaggacatt gcgattcaag gcgtgcgtaa cgccgttgtg cacggcatcg
     3241 agctgccggc cgcacgcgtc gccgcgggca agccgaagca aggcacggta cgcctgacct
     3301 tccaagatgc gggcgaggcc ggctacaagc tcatcatcga ggacgatgga cagggtcttg
     3361 gcatcgaacg catcaaggag gtggcgttga agaaaggcat catcagtgcc gagcgggcgc
     3421 agagcatgga cgcgaaacag gtatttgggc tgttgttcaa gtcggggttc tccacggtcg
     3481 agaccgcaac caaggatgca ggccgaggtg tcggcatgaa tctcattgcg gatgtcacca
     3541 atcagttggg cggacgcgtc tcgcttgcca ccagcaacgg taaatttacc cggctcacca
     3601 ccaccttgcc gcgctcgccg aagcgcgccg cagacgcgga ggccgcgtaa tgccgatcga
     3661 agccgtcgcc aaggcctcgc tcaagctgat gatcgtcgat gattcgaata tcatccgccg
     3721 ccgcatcgag cgctcgcagc agatcgaccg cttggaagtg gtcggcgccg cgagcaatgg
     3781 ccgcgaggcg gtggagctgt ttcgccgcac ccaacccgac gtcgtgacca tggatctgac
     3841 catgcccgaa atggacggcg tcgagtgcgt acaaaagctg gtggcgatca atccgggagt
     3901 tctgatcctg gtggtctcgg cgctggcgga caaagcgacg gcggtcgacg ccatcgagaa
     3961 gggggcaaac ggctttctat gtaaaccgtt taccgaccgt cagctcaacg acgcactgga
     4021 agagctgttg tcggaggcgt gatgctcaaa gaagctgaaa tacgcacctt catcgacggc
     4081 acgaccaatt atttcgaaat agccgcccag caggcggcga ccgtcggatc gccttacctc
     4141 gtgacggatg gaaatcccgg cgcctacgag tacacgggcg tgatcaacat ttccggcgcg
     4201 cgcaccggca tcgtgtactt caccgcgccg cgcggcatgc tgaccgtgct gctcatgcga
     4261 atgcaggaaa ccgataccag cgatgagaac atcaaggatc tcgtcggcga ggtcgccaac
     4321 acgatttccg gcaatgcacg ccgcgacttc ggcaagaatt tccacatctc ggtgccggtg
     4381 gttcatgaca ctgagaaagt cgcggcgccg cacagccggt cctacgtgat tccgatcaac
     4441 tggcgtacgc actcggccaa attggtcgtc tgcttggaat gagccagcct acagcgatct
     4501 caagaaattc aagatatctt gttgatgctg cgcactcaga ttgttgaacc gctgtatgac
     4561 gccgttggcc tcgctgcccg gactttcatg cgcctggatg gcggtcagca aatcattggc
     4621 ccgtccatcg tgcaggaaaa agacgcgttg accgactccc cacagcgggg cggtgcgaaa
     4681 ttcatcgggc cccgccgcac cctgcgtgat gccgtcggcc aggccctggc ccatgtggtg
     4741 caccaacagg tcggagaata gattgaccgg ctgattcgac agagccgggc tcgggttgtt
     4801 cgaggagccg ctcgagacgg cgcgtccggt gttaaaggac ggcgtatgac acaatgcgca
     4861 gccggtactc gcgaactcgg cgcggccgtt gagggttgag gcattgctcg ccgccggcgc
     4921 cggaggcgcc aacaagcgca tgaaattggc gaatgcctcg atgtccgaga gcacggccat
     4981 ggcgttcccg gtgaccgtta gcggcatgtt gttggtatcc tcgggcgtgg aggggtacgt
     5041 gcggcaggcg gccgtttcat cgcgctcttg cggaaacagc tgattcgaca cacccatctc
     5101 cacgttgtaa gcctcgccgg cgaacatcag cagcgatttg ttctgtgcct tccagccgaa
     5161 gcgtgtgatg gtgccgtcgt tggcgctcga gttcacgttg ccgcccagga tggcgttggc
     5221 gcggccgcgc acgcccactt ggctcttggc ggcggcattc gtctgcgcgt tcgcgagaat
     5281 ggcggagtcg ggaatggctt cgatgagacc ggcaccgaat accggggtcg ggatgcgaaa
     5341 tacgatattg cggttgccgc cctggcccgt caaaggattc ccagccggca gaaagttcgg
     5401 ctgcgcgata ttgcaggtgc ccgcatcgct gcggccggtc accacgaata aattatgcac
     5461 gccgccatcc ggcgcgccgt tggtctgcac gaagcgcgcc tcgcggatcg ggccgttggc
     5521 cgcgttgaac cagggcatgg tattggtcgc gccatcggcg ttcgcgacgg cgctcagggg
     5581 attttgcggc gggctcgagc cgccgacgtt cggctgcgtg tggcaggatg cgcactgatt
     5641 cgaattgaag cgcgggccga ggccgttgcc cgtgccctgc tgcgaatttc caccgttcac
     5701 gacttcgatg aaattaaacc gcgccatccc gtcgaggaag aaattcatct cgtcggtgcc
     5761 gatgccggcg agcggttgcg gcgtaccatt gtcggtcgcg gcgtggcgaa caccagggtc
     5821 cgtgggcaga gcgagtgcca ccggcgtttg aattaatgca gcgctgagca ggcctgccaa
     5881 ggcggccctg cggtcgagat cgtggtgcat agtgtctcct tgggtcacgg tgggtgtcga
     5941 cacaagtcat cggggaaccg atatacgcgc gcagtttttt tgtgtcgacg tgatgcactt
     6001 cggagacgtc agcgggcagc gacagaaact caagcgggcg cccgcctggc gagactcgag
     6061 aactggcgtg tgtcgcaacg ctgcggcatg ctggcggagt tttcaaaaaa aaaggggaag
     6121 cgggtatgct gaatcgacgc gaagtattga gtggtgcggc cgcggcggcc gcactcggcc
     6181 gctggtcgca ggcacgcagc ggcacgccgg cgtcgggcga attctcgaaa tcgcaatacc
     6241 agcgggcgat cgtgatcgat gccctgggcg ccgtcggcga gaacgatccg gcggccgcgg
     6301 acgatgcgcc gttgaccgcg cgcgggctcg aggatgcgcg cgaatccggt gtaacggccg
     6361 tcaatttcac catcaatgaa ccgggcaacg ggccggacag gttcaagatc gcgattacca
     6421 atatcgcggg cgtcgagcat gaattcacgg cgcacccgga gtttctcgtc aagattcagc
     6481 gcgccgcgga tattcaggcg gcgaaagcca gcaagcgcat cgggatcatc tacggctgcc
     6541 aggacgtcac catgctcgag ggtgatatca acaagttggc gacgttcgcc gacctcggct
     6601 tgcgcatcgt gcagccgact tacaaccgcc gcaatttgct gggcgacggc tgcctggaga
     6661 aggcagacgg cggcttgagt aaaatgggtt acgatctaat cgcggagata aaccgcctca
     6721 atctgttgct cgacttgagc cacgcgggcg tgcgcaccat tgctgaaggt attgcggcct
     6781 cgaaggcccc gatggcgatc acccacacgg gctgccgggc gctcgtggat ttgccgcgca
     6841 atacgccgga caccgagctc aaagccttgg ccgaccgcgg cggagtggtc ggcatctact
     6901 tcatgccgtt tctgcgcgcg tccgggcaac cgcacgcaga ggatttgatt cgccacatcg
     6961 agcacgccgt caatctgtgc ggcgaggagc atgtcggact cggcaccgat ggcagcattt
     7021 ccgccgtgcc gctgaccgac gcgtatcgcg agtggtttcg caagagcgtc gaagcgcggc
     7081 agaaagccgg catctccgcg cctggcgaat ccgctgacgt gttcaccctc gtgcccgaat
     7141 acaacgatcc gctgcgcttc ctgcacctgg cgaacgatct ggcgcgccgc ggctggccgg
     7201 gcagccgcat cgagaaattg ctgggtgcca atttcgcgcg cctgtttgcc gatgtgtggg
     7261 atgcgcaccg cgcctagtgc gttctaggtc gagcgcaggc gttcgacctt gcacaagccc
     7321 aagctggcgc gcaccttgaa acccatggag tcgtagaggc tgcgcgcagc gctattgcct
     7381 tcgctgacat gcaggaagga gcgcacgccg cggcggcgct gccggctcag caacgcgcgc
     7441 gtgagccgcc gcgcatgccc ctggccggcg tgctccgggt gggtgcacac tccgctgatt
     7501 tcctgcagac catcgagcgc caggcgttcg ccggccatcg cgaccagctt gccgccggag
     7561 aaaaccccga gatacaggcc gagttcggcg gtgcgcgcgc gaaaaaaatc cgggaacgcg
     7621 atttgagtga gttcctgcat ggccgcccga tcggcttcgc cgagaacggt gccggcttcc
     7681 tcctcagctg gaccgaggtc gagagtcggg aacagctgca aaatccgtga gtgggaaaca
     7741 aatcgccagc gctcgggcag agaagccggg cagatgccga tgaaatacgc cgactcgccg
     7801 aggcccgccg cacgggcagc gtcgagatcc ggcacgacca gcgcgtgacg gatcgcagcg
     7861 aacggtgcga tcgacggcgg ataccacagc aactcgccga cactccccgc caacccgcgg
     7921 tgcggcccgc agagcgcgga ccacaccggg ttgtcgagcg ccgcatcgat ggacgcaatt
     7981 ggatggatcg ccgtgcctca caccgctgcc tttagcggga ttttatacct cggcgccgag
     8041 tgcttgcgcg ataaattggg ctgcagatcc tcgcgcgcct tgaccaggcg ctgccaatcg
     8101 gcggcttccg gcagcgacgg cagcgtgacg cgctcgccct ggtcgaagcc ggcgagcgcc
     8161 gcatccacca tctcatcgac gttcatcacg atatcgggcg gaagatcctt cagttcgacg
     8221 ccggacccgt tccacagagc cgtgccggtg gcgcccggca gcacgacctg aacacgcacg
     8281 gcggaggcgg cgagttccag cgccagcact tcgctgaaat gcagcagata ggctttcgtg
     8341 ccgccataga ccgagttgcc cggctgcacc atctgcgaca tgacagaggc gatgttgatg
     8401 atgctgccgc gatttcgttt gagcaggccc ggcagcgccg cgtaggtcag gcgcgtcggc
     8461 gcgacgatat tcaactggat atcgcgcgac aggcggccga tatcggcctt gacgaaggcg
     8521 cccaaggtgg cggcaccggc attattgatg agcagcgtga tgccggcatc gtcgcgcagc
     8581 acctgctcga cgcgctgcac atcggcgtcg accgtaaggt ccgccggcag cacggtgatg
     8641 cgtactgcgc tcgccgcgcc gagcgttgcc gcgagctttt ccagattcgg cttgttgcgc
     8701 gcgaccagta tcaggtcgta gccgcggcgc gccagccggt tcgcgtaggt ggcaccgata
     8761 ccggtcgatg caccggtgat cagggcggtg cccaaactgt ttgtcgtact catggggacc
     8821 tcgatgaatt gtggatgata atcataatcc acaaatactg tagccggccg agcacctaga
     8881 tgtcaagtaa catccagatg gagacgcgcc gcggccgctg tgctatatag gtcgcccatg
     8941 gggcactcga aagccagcaa ggaacagacg cacgcgcgcc tggtcgccgc cgccgccgcc
     9001 cgcttcaagg agcgcggcgt cgatggcatc agcctggccg acctgatgaa agaccttaag
     9061 ctcacccacg gcgggtttta taagcatttt gactcgcgcg atgagctcgt gaccgaggcg
     9121 ctcgacttgg cgctcaagca aagcggcgcg agcgtgcgcg agcggttgtt tggcggcgat
     9181 gagccggatt tgtcgcgatt cgtcgatttt tatctcgcgg aagcgcatcg cgacgggcgg
     9241 gcggacggct gtgcggtcgc ggccctggcg ggcgacgcgc cgcgcaaggg tgcggccgtg
     9301 cacgcgcaat ttcgcgcgca catcgagagc aatctcgcca atctggtcga ggcgctcgcg
     9361 ccgggcggct cgcccgcgga gcgacgcgcg tcggcgctga tggtgttgag cgccctgtac
     9421 ggcgccttga tgatggcgcg ggcggtgggc gattcgcctt tgtcgcgcga gatcctgcgc
     9481 acggcacgca agcggatacc ggctctcggc ggcgtgcgca gcctgcccga gaaaaagacg
     9541 cggaacccgc attgagcgct gccggcgcaa tctcgcggac gtcgcggacg tcgcgggcgt
     9601 gggcggttgg gttgagtctc ctcagctgcc tgccgctgcc tgccgctgcc ggcgatgctg
     9661 ccatcggcgc gacagtcccc gcgtggctgt ttccgcataa cgcgcctagc ctcgccgcag
     9721 acaacacggt cagcgccaag ctcgagcatc tcaagggcag ccgcctcggg tacacgcggg
     9781 cgcagttgac cgatctgttc ttcgcgcccg actggcatcc ggagtcgcat gcagcgccgc
     9841 cgcagatcgt cctgcacggt cgcgcgcccg gcgtgtatgc ctgcggttac tgccacttgc
     9901 cgaccggtca agggcggccc gagaacgcgc cgctcgcggc actgccggcc gcttacatcg
     9961 tccagcaagt gctcgatatg cgcaccgccg cgcgccacag cgcctggcac ggtgccgcat
    10021 ttgttcccgt cgacctcatg cgggatgtgg cggcgaatgt cagcgacgcg gaattggcgg
    10081 cggcagcggg ctactttgcc gcccagccgc tgcgggggcg cgtcaccgtg atcgagcgcg
    10141 cgacgattcc tcggatgcgc gtggcggcct ggatctacgc tcgcgattca cgcggcggga
    10201 cggagccctt gggtcaacgt ctgatcgaat gggcgccgga cctcagccgc catgagcgcc
    10261 gcgacgaaga cctgcgctac acggcgttcg tgccgccggg cagcgtgaat cgcggacgcg
    10321 agattgcgat gcatggcctg cccggcgtcc aggcgtgcgc aggctgccac ggccggcgct
    10381 tgcaagggac cggactgttg ccgccgctgg cgggacgttc tcctacgtac ttgctgcggc
    10441 aactggtcgc atttcaaacc aaggaccgcg cgggattgat ggccgtgccg atgcaggccg
    10501 tggtcgcgaa catgcagctg ccggatctga tcgcggtggc cgcctacgcg gcgagtgcgt
    10561 cggcgaacta ggacacgctt tccgcgctat agcgcgcgag cagcccggtg cggaccgcgt
    10621 gccgatagac ccgcgcataa aagtacgccg ccagcagcag ttcgagagcg gcgaccagcg
    10681 ctccctgcag caacgaggct gcgggaaagg agcggtgggc gagcaagccg cgcatgccct
    10741 cgaacacata agcaggcggc agcgcgtacg acacccatcg catccacgcc ggcagcacgg
    10801 ccagcgggta gaacaccgcg gcgaacggcg acagcagcgc cggcagcggc cacagcagcc
    10861 attcggacgc cggacccagc cgcaggacca ccgcgctgcc gaaaatgccg agcgagatgc
    10921 cgaatacgaa cagcaccagc agaaacgccg ccagcggcag tcccatcgcg aaaaactcga
    10981 gatgaaacac gcccgtcgcc agcagcaaca tgacgagcag tccgacgctg ctggtggcga
    11041 cgctgctgat gacgagtccg gcaatgtatt cggcgatcga cagcggcgag gcgaacagat
    11101 tcaggaaatt gcgcgaccag acatcttcga ggaacgcggt ggtcacgccc tgcatgatgc
    11161 gtgtgcagaa atcccacaac agcacgccgc cgagcaaggt gggaacgaag tccacgccgc
    11221 tgccgctcac ggtgttgagg tatcgggtaa tgaaacccca caacacgata tcgatcgcga
    11281 cccacgtcac catcggaaac aggcgcaccg gactgccgcg cagcaaatag atctggcgca
    11341 gcacgattcc agcgatggga cgcagggtca tggggcgccg gcggctgcc
//
LOCUS       DASWZT010000487         6022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_23488, whole genome shotgun sequence.
ACCESSION   DASWZT010000487 DASWZT010000000
VERSION     DASWZT010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6022
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23488"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VGE92_14010"
     CDS             <1..606
                     /locus_tag="VGE92_14010"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY1314990.1"
                     /translation="LMSLPLAFNTRLDTIPAQVPYLTAEPQRTANWMQRLQAYSGLRV
                     GIAWQGNLAVEKLIWARGRSIPLAALEPLAQLPGITLVSLQKGPALEQLRDVAFAGRI
                     IDFSAALDRGPDAFLDTAAMMAGLDLVISSDTSIAHLAGALGRPVWTVLAASSEWRWG
                     LERSDSPWYPTMRLFRQTSDGDWSAVVAAIAEALENLSAGA"
     gene            complement(590..1051)
                     /locus_tag="VGE92_14015"
     CDS             complement(590..1051)
                     /locus_tag="VGE92_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314991.1"
                     /translation="MTGSVYRSLGLWLTAICLPAFTEAENLDGALRHCATESDLVQRL
                     ACFDAIVSTLPQVEADRFGMTAEIEHKRDPQAVPQAKDEVLSGKISGLRQASQDQLIF
                     TLDNGQVWIQTETRPNVQFALSEDVHIEHGSMSSLWLVADKHRKVRVKRLR"
     gene            complement(1048..2361)
                     /locus_tag="VGE92_14020"
     CDS             complement(1048..2361)
                     /locus_tag="VGE92_14020"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEY1314992.1"
                     /translation="MPATVNATPLSETEARTLADAIEQVSTSQPPETALKFVEMAVAR
                     APLQPLVLNAAGGYMYRSGNAPRARNLFEKAVAADGNSKVLWLNLANACRALGDKNSE
                     AQALEKALAIDPRYILALLCKAQLMEGLGQSKKAFFFYEAALDSVASGAPVPQQAAAA
                     LTRAQEVVLANRKELERFLAQQIAAVSLQHAAADQRRFEACRDVYLRKRRVYRNQPKQ
                     ILFPYLPEIEFFRREDFPWLDVVEAATEEIAAEALAALASDRAGFKPYVDFPPGAPID
                     EWTSLNHSSKWSVYALWKDGEQIDDHVRKCPKTAAVLAQLPLCDIPRCAPGAFFSMLT
                     PRTRLPPHTGTTNTRSIVHLPLVIPEGCGFRVGAEARSWKKGQAWVFDDSIEHEAWND
                     SDEIRIILIFDIWNPLLSVAERDLVRALTLGIGRYYGVDAPVLSS"
     gene            2532..3584
                     /locus_tag="VGE92_14025"
     CDS             2532..3584
                     /locus_tag="VGE92_14025"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retroviral-like aspartic protease family
                     protein"
                     /protein_id="HEY1314993.1"
                     /translation="MGAAFAHSPLRLANRKSVRGPLWSLLAGMGIAALAGPAAAGPSG
                     EQPSSAALSRDQASLLALPTTRDHIGRVVVQASVNGKGPFRFIVDTGATHSTITPDLV
                     RALGLKPADLPTVVLNGITGTTQVSAVNLDTLQTGDLTIDGVLVPVVSGPIMAGADGI
                     FGAAGLTEKSLSVDFLHNRVEISGGVQAAVRAQALRIHATRVAQGLMVLAMQVGGVHA
                     LAVIDTGSERTLGNLALRDALSERRRPGTVAQVTSVYGATAQVERGEICRAPTISIDS
                     LRINDVQVVYGDFHVFRVWDMQEKPAMIIGMDVLGTLASLGIDFKNQVIYISSSVATS
                     QTNQPTGSLASPNQIR"
     gene            complement(3603..4802)
                     /locus_tag="VGE92_14030"
     CDS             complement(3603..4802)
                     /locus_tag="VGE92_14030"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEY1314994.1"
                     /translation="MSKLVRGFGSITLLMLAFVARAESVANWADTTTDIATDGPNTIR
                     TMALAQRAVYEAVNAITARYPRDRVDLGPSQDASIDAAIAAASRTVLLHEASALRDRT
                     EAAYARVLATIAAGDGRTRGISLGERAAADTLAKHSDDIGNTEPYRPLTSAGVYVPTT
                     FPLGYAFAQHRPWFMKSAAQFRPEPPPALTSALWARDYNEIKIVGSATSRVRTPEQTA
                     IAQFWATALPDVHMGVVHSIAAAPGREVTRNARLYAAVTAAMNDVEIAVLEAKYHYNF
                     WRPITAIRNGDRDGNPATERDPDWTPLIATPLHPEYPCAHCAIASAIATVIRAEVGRE
                     PLPALMSTSNTAPGVTRQWTRTEDVVKEVSEGRILDGVHYRNSTAVGTRMGAQVGALV
                     AAAYGLD"
     gene            5360..5914
                     /locus_tag="VGE92_14035"
     CDS             5360..5914
                     /locus_tag="VGE92_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314995.1"
                     /translation="MNALGMKSLVRLLLVLFAVVSAGLFTAVPNVGEADEPPSQPSAA
                     ASAQPVAQSPTVAPTDPNTTRAATADPSSTGKAITGAQPGGIATSPVAANAQPVANKP
                     GSKVLVDDTVTDAQLKQILRKGYQPESQARGNEVYYCRSERELGSRFETKVCRTAARI
                     LQEEQLGKDATTNVERTGGNKALP"
BASE COUNT         1069 a         1897 c         1886 g         1170 t
ORIGIN      
        1 ttgatgagtc ttccgctcgc attcaatact cgactcgaca cgattccggc tcaagtcccg
       61 tacctcacgg cggagccgca gcgaacggcc aactggatgc agcggctgca ggcgtactcg
      121 ggtctgcgcg tgggaatcgc ctggcagggc aacctcgccg tggagaaact gatctgggct
      181 cgcgggcgct cgattccact tgcggcactc gagccgctcg cgcagctgcc cggtatcacg
      241 ctcgtgtcgc ttcagaaggg acccgcgctc gaacagctgc gcgacgtcgc gttcgccggg
      301 cggataatcg atttcagtgc cgctcttgat cgcggaccgg acgcgtttct cgatacggcg
      361 gccatgatgg cgggtctcga cctcgtgatc agctccgata cctcgatcgc gcacctcgcc
      421 ggagcccttg gccgtccggt gtggacggtc cttgccgcct catcggaatg gcgctggggg
      481 ctcgagcgca gcgacagtcc ctggtatcca accatgcgac tgttccggca aacgagcgac
      541 ggcgattgga gcgcagttgt ggccgcgatc gcggaagctc ttgaaaacct cagcgcaggc
      601 gcttgacccg aaccttgcga tgtttgtcgg cgaccagcca cagggagctc atcgatccgt
      661 gctcgatgtg cacgtcctcg ctcagcgcga actgaacgtt gggacgcgtt tctgtctgga
      721 tccagacctg accgttatcg agtgtgaaga tcaactggtc ctgggatgcc tgccgcagtc
      781 ctgagatctt cccgctcagc acttcatcct tcgcttgcgg cacggcctgc ggatcgcgct
      841 tgtgctcgat ctctgcggtc atgccgaagc gatcggcctc gacctgcgga agtgtgctca
      901 cgatggcatc gaaacaagcc agccgctgca ccaggtcgct ctctgttgcg cagtgtcgca
      961 aggcgccgtc caagttctcg gcctcggtga atgcaggcaa gcagatcgcg gtgagccaca
     1021 gccccaggct acgatacacg gagcctgtca tgaactcagc acgggagcgt cgacaccgta
     1081 atagcgtccg atgccgagag tgagggcgcg cacgagatcg cgctccgcga ccgaaagcag
     1141 cgggttccag atatcgaaga tgaggatgat tctaatttcg tcgctgtcgt tccaggcctc
     1201 atgctcgatg ctgtcgtcga acacccaggc ctggcccttc ttccagctac gcgcctccgc
     1261 gccgacccga aagccgcagc cctcgggaat cacgagaggc aggtggacga tcgatctcgt
     1321 attggtcgtt ccggtatgcg gcggaagtct ggtgcgcggc gtgagcatgg agaagaaggc
     1381 gccgggcgca cagcgaggga tgtcgcatag gggtagctgc gccaacaccg cagcagtctt
     1441 ggggcacttc cgcacgtgat cgtcgatttg ctcgccatct ttccagagcg cgtagacgct
     1501 ccacttcgac gagtggttga gcgatgtcca ctcatcgatc ggcgcgcccg gcggaaagtc
     1561 gacgtagggt ttgaaccctg ctcgatcgct ggcgagcgcg gcaagggcct cggcggcgat
     1621 ctcctccgtt gccgcttcca caacgtcgag ccagggaaag tcctcgcgcc ggaagaactc
     1681 gatctcgggt agatacggaa acaggatctg cttcggttgg ttacgatata cgcgacgctt
     1741 tcgcaggtac acgtcgcgac aggcctcgaa tcgccgctgg tcagcggcgg cgtgctgcaa
     1801 actcacagct gcgatctgct gcgccaagaa cctttcgagc tccttgcggt tggcgagcac
     1861 gacttcctgg gcacgcgtga gcgcggccgc ggcctgttgc ggtaccggcg cgcctgatgc
     1921 caccgagtcg agcgccgctt cataaaaaaa gaaggctttc ttgctttggc cgaggccctc
     1981 catcagctga gccttgcaca gcagggcgag tatatagcga gggtctatcg cgagtgcctt
     2041 ctccagcgcc tgcgcttcgg agttcttgtc gcccagcgct cgacaggcat tcgcgaggtt
     2101 aagccacaaa accttggaat tgccatccgc cgccaccgcc ttctcgaaga ggttccgagc
     2161 gcgcggcgcg ttgccgctcc ggtacatgta tccccctgca gcgttgagga cgagcggctg
     2221 cagcggcgcg cgcgccacgg ccatctcaac gaacttcagc gccgtttcgg gcggctggct
     2281 cgtggacacc tgttcgatcg catcggcgag ggtgcgggct tcagtttcac tgagtggggt
     2341 cgcgttcacg gtggcgggca cgacggtctt tttgacactg gcactcccag cgccggtaaa
     2401 gtgtagcgcc tccgcaagac ctttgtcagg cgtgcatcgc ttgacgattg cgtctcaccg
     2461 ctgttctcga gaagtgtgtg cgcactggcg cgcgcacgaa gagcgccacg gttcgtgcta
     2521 cgctgcggcg cgtgggtgct gcattcgctc attcgcctct tcgactggcg aaccgcaagt
     2581 cggtacgcgg tcccctgtgg tcgctgcttg ccggcatggg aattgctgcg ctcgccgggc
     2641 ccgccgccgc ggggccgagt ggcgagcaac cgtcgtccgc ggcgctcagc cgcgatcagg
     2701 cgtcattgct cgccctgccg acgacgcgtg atcatattgg ccgtgtggtg gtccaggcca
     2761 gcgtcaacgg caaaggtccg ttccgcttca tcgttgacac gggcgctacg cactcgacca
     2821 tcacacctga tctggtgcgc gctctgggcc taaaacccgc tgatctaccg acagttgtac
     2881 tgaacggtat caccggcact acgcaggtgt ccgccgtgaa tctcgacacg ctgcagacgg
     2941 gcgatttgac tatcgacgga gtactcgtgc cggtggtgtc ggggccgatc atggccggcg
     3001 ctgacggcat cttcggcgca gcagggctga ctgaaaagag tctgtcggtc gatttcttgc
     3061 acaaccgcgt tgagatttcg ggcggtgtgc aagcggcggt acgtgcgcag gcgttgagaa
     3121 tccacgccac gcgcgtggcg cagggcttga tggtgctggc gatgcaggtc ggtggagtac
     3181 atgcccttgc ggtcatcgac accgggtctg aacgcacact cggcaatctt gcgctgcgtg
     3241 atgcgctgag tgagcggcgc cgacccggta ccgtggccca ggtgacctcc gtgtacggcg
     3301 ccacggcgca ggttgagcgc ggtgagattt gcagggcacc caccatcagc atcgattcat
     3361 tgcgtatcaa cgatgtccaa gtggtctatg gcgacttcca tgtattcagg gtgtgggaca
     3421 tgcaggaaaa gcccgcaatg atcatcggaa tggacgtgct ggggaccctc gcttccctcg
     3481 gcatcgattt caagaatcag gtcatctaca tctcgagctc cgtggcgacc tcccagacga
     3541 accagccaac tggatcgctg gcgagtccaa atcagatacg ctagtgcggc acccgggaat
     3601 ggctagtcaa gtccgtaagc cgccgccacc agcgctccga cctgcgcacc catacgggtc
     3661 cccaccgcgg tggagttgcg gtagtgcacg ccgtcgagga tgcggccctc agatacttcc
     3721 ttgaccacat cctcagtgcg cgtccactga cgagtcactc caggcgcggt attgctggtg
     3781 ctcatcaatg ccggcagggg ttcgcgaccg acctcggcgc gaatcacggt ggcgatggcc
     3841 gaggcaatag cgcagtgcgc gcatggatat tccgggtgca gcggcgtagc aatcagcggc
     3901 gtccaatcgg gatcgcgctc ggtcgcgggg ttgccgtcgc gatctccatt gcgtatcgcc
     3961 gtgatcggac gccagaaatt gtagtggtac ttcgcttcaa gaacggcgat ctcgacatcg
     4021 ttcatcgctg cggtcacagc ggcatagagt cgcgcgttac gcgtcacctc ccgtcctggc
     4081 gcagcagcga tggaatgcac cacgcccatg tggacatcgg gcaacgccgt cgcccaaaac
     4141 tgggcgatcg cggtttgctc gggagtgcgc accctgctgg ttgcggagcc gacgattttg
     4201 atctcgttgt agtctcgcgc ccaaagtgcg ctcgtgagag cgggcggcgg ttccggccga
     4261 aactgtgccg cgctcttcat gaaccagggc cggtgctgcg cgaatgcgta cccgagtgga
     4321 aatgtcgtcg gcacatagac acccgctgag gtcagcggcc ggtaaggctc ggtgttcccg
     4381 atatcgtcac tgtgcttcgc cagcgtatcc gccgcggcgc gctcgccgag gctgatcccc
     4441 cgcgtgcgcc cgtcgcccgc cgcgatcgtc gcaagtaccc gcgcatacgc agcctcggtt
     4501 ctgtcccgca gtgcggacgc ctcatgtagc agcacagtgc ggctcgctgc agcaatcgcc
     4561 gcgtcgatcg aggcatcctg gctgggcccc aaatcgactc ggtcgcgcgg atagcgcgcg
     4621 gtgatcgcat tcacggcctc gtacactgca cgctgtgcca gcgccatggt tcgaatcgta
     4681 ttcggtccat ctgttgcgat atcagtggtc gtgtcagccc aattggcgac cgactcggca
     4741 cgcgcgacaa aggctagcat caacagggtg atcgacccga atccacgaac cagcttgctc
     4801 atgcttctcc catctcacga gcgtccaatg tggacgctcc ttgcgcaggc gcgccggctc
     4861 tgcgtcgcgt cagttatgag tgacaatgat cgaggggtcg agcaagcgaa gcgcctgggc
     4921 tgagtccgtt gccccggttg tcttgatatc ttgatctgtc catgtgcgac cgaagcccgc
     4981 gcacttatcc gggctcaccg gaattcgcgt ggctgtattc gatacgcatc ccacaggcgt
     5041 caggttgggc gccgcagccg ccttcgacgt tgctgctcct gggctcgaag ctgtgctcac
     5101 gcaggccgcg acgcacagac tgagccacgc gccgatcagg tgattcttcc acataattat
     5161 cccctccaaa ttatcctacg cggctgcgcg cagccgctgc aacgccgttc accgcaacag
     5221 catcccgctt catggtcgca tcggtccgct ccgccgcaaa ttgtgcggca cttgcgctcg
     5281 gccgactaga ctcgccgcat cccatgccta cccgaactgc atttttacag ctgatcgatc
     5341 caaccagaag gggtgtgggg tgaacgcgct cggcatgaag tcgcttgttc gcctgttgct
     5401 cgtcctcttc gcggtggtga gcgctggtct atttaccgcc gtgccgaatg taggtgaagc
     5461 ggacgagccg ccatcgcaac cgtccgcagc ggcctctgcg cagcctgttg cgcaatcccc
     5521 aaccgtggcg ccgacagacc cgaacacgac gcgggcggca actgcggacc cctcctccac
     5581 cggcaaagct attacgggcg cgcaaccggg cggcattgcg acatcgcctg ttgccgctaa
     5641 cgcgcaacct gttgcaaaca agccgggaag caaggtactg gtggacgaca cggtcacgga
     5701 tgcgcagctc aagcagatcc tgaggaaggg ttaccagccc gagagccaag cgcgtggcaa
     5761 cgaggtgtac tactgtcgca gcgagcgtga attgggctcg cgttttgaaa ctaaagtgtg
     5821 caggaccgca gcacgaatcc tccaggaaga gcagctgggt aaggatgcga cgaccaacgt
     5881 ggaacgaacc ggtgggaaca aggcgctgcc atgattgcga cgctccaata gcgcatcatt
     5941 gtgcctcctg cgtacggcgc tgcgattact caggtttgct ggtcagtcgg attttcgcga
     6001 gactcgctgt ggattggccg cc
//
LOCUS       DASWZT010000488         1735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_61907, whole genome shotgun sequence.
ACCESSION   DASWZT010000488 DASWZT010000000
VERSION     DASWZT010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61907"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..1432)
                     /locus_tag="VGE92_14040"
     CDS             complement(110..1432)
                     /locus_tag="VGE92_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEY1314996.1"
                     /translation="MRPKQHATTGSADLFRARLDQIINLKHELVALAGKLDWTWIDGE
                     IAPLYSDQGRPGIETRFVIALLLLKHIYGLSDEGVCERWVHDPYFQYFTGEEFFQHEF
                     PHERSDLSHWRKRLGDKLELLLAESLRVAHASGALRTKDLARVTVDTTVQPKNITFPT
                     DAKLLHAAIKALNRMARKHGVRLRQSYLRIAKHAAMMAGRYAHAKQFNRHRRQLRILR
                     TRLGRIIRDIHRKIAGQPVLEGAFEWPLARASQIRSQQQRQRGWKLYSFHAPEVECIG
                     KGKASAPYEFGVKVSVTTTNARAPGGQFVLHAKALPGNPYDGHTLRSAIENTERLTGR
                     QIERAYVDKGYRGHDAQNPHRIFISGQKRGVFGAIKRELRRRSAIEPVIGHMKAEGHL
                     GRCYLKGTGGDAANAILTAVGHNFRLILAWLRILLRLILAALHSAVTV"
     gene            1528..>1735
                     /locus_tag="VGE92_14045"
     CDS             1528..>1735
                     /locus_tag="VGE92_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1314997.1"
                     /translation="MQSQTSMFLKNRFNIKDLTLVHGRLSMLRIQHGHEVVYRLITGS
                     QMNVLLLANTPAAINMTKAREIYDA"
BASE COUNT          330 a          499 c          546 g          360 t
ORIGIN      
        1 tcaaaaaatc cgtcaggtca tgcttggctg acttggcttt ttttggacca agcgttgtga
       61 tagaatgagc ttagaatgtc gtccgttaag aagccatttt ggggttaggc tatactgtga
      121 cggcgctgtg tagcgcggcc aggatgagac gcaaaagaat cctcagccag gcgaggatga
      181 ggcggaagtt gtggccgacg gcggtgagga tggcgttggc ggcatcgccg ccggtcccct
      241 tgagatagca gcggccgaga tggccttcgg ctttcatatg gccaatcacg ggctcgatgg
      301 cggagcggcg gcgcagctcg cgtttgatgg cgccgaacac gccacgcttc tggccggaga
      361 tgaagatgcg gtgtgggttt tgcgcatcgt gtcctcggta tcctttgtcg acataggcgc
      421 gctcgatctg gcgcccggtg agcctctcgg tgttctcgat tgcggaccgc agggtgtggc
      481 cgtcataagg gttgccgggc agcgccttgg cgtgcagcac gaactgacct cctggcgcgc
      541 gggcgttagt ggtggtgacc gagaccttga cgccgaactc atagggcgcg ctggctttgc
      601 ccttgccgat gcactcgacc tcgggagcat ggaacgaata cagcttccag ccgcgctggc
      661 gctgctgctg agaacggatc tgggaggcgc gcgccagcgg ccattcgaat gccccctcca
      721 agaccggctg gccagcgatc ttgcggtgga tgtcgcggat gatgcggcct agacgcgtgc
      781 gcagaatgcg caactgccgg cgatggcggt tgaactgctt ggcgtgggcg tagcgccccg
      841 ccatcatcgc ggcatgcttg gcgatgcgca gataggactg tcgcagccgc actccgtgct
      901 ttctggccat gcggttgagg gccttgatcg ccgcgtgcag cagcttggca tcggtcggga
      961 aggtgatgtt cttgggctgt acggttgtgt cgaccgtgac ccgtgccaga tccttggtgc
     1021 gcagcgcgcc gctggcatgc gccacccgca ggctctcggc cagcagcagc tcaagcttat
     1081 cgcccagccg cttcctccaa tggctcaggt ccgagcgctc gtgcggaaac tcatgttgga
     1141 agaactcttc cccggtgaag tactggaaat acgggtcatg gacccagcgc tcgcacacgc
     1201 cttcgtccga cagcccgtag atgtgcttga gcagcaacag cgctattacg aagcgggtct
     1261 cgatcccggg ccggccctgg tcgctataga gcggtgcgat ctcgccgtcg atccacgtcc
     1321 agtcgagctt gccggcgagc gccaccagct cgtgcttcag gttgatgatc tgatcgagcc
     1381 tggcgcggaa aagatcggct gatcccgttg tcgcgtgttg ctttggccgc atcgtgccct
     1441 ccgatgcagc caagcaatca cgcttcacgt cacaagggaa tcccaaaaat gaaattgcag
     1501 gattccaggc cctcaggccg ctaaatcttg caatctcaaa ccagcatgtt cctcaaaaac
     1561 agattcaata tcaaagacct gacccttgtt cacggacgac tatcaatgct gcgaatacaa
     1621 cacgggcacg aagtggtcta tcggcttata acgggatcgc aaatgaacgt ccttttgctc
     1681 gcgaatacgc cggctgcgat caatatgaca aaggcccgcg aaatttacga cgctg
//
LOCUS       DASWZT010000489         2984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_4020, whole genome shotgun sequence.
ACCESSION   DASWZT010000489 DASWZT010000000
VERSION     DASWZT010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4020"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VGE92_14050"
     CDS             complement(<1..481)
                     /locus_tag="VGE92_14050"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEY1314998.1"
                     /translation="MSRQRFLTLLIAALIAIGGALYLSAQRNQQRETIGVALLPALAG
                     EINTVTQLSVRRGSATPSVTLHSRAGRWTVAERGDYPADVPKLRKLLLALSDAKIVEE
                     KTSNPANFSVIGVDDPSSATASGAEISISAQDGKHAVIIGKPVGEGNFARRVGENRSY
                     "
     gene            complement(478..2385)
                     /locus_tag="VGE92_14055"
     CDS             complement(478..2385)
                     /locus_tag="VGE92_14055"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEY1314999.1"
                     /translation="MNESRWRRWGFGVGGLIALAVLFLGVVMLSNLGLRGMRIDLTQN
                     RLYTLSPGTQQVLAELKEPVNLYFYFSREAAAKQAPLLMPYATRVREFLEELAARANG
                     KIHLRIIDPQPFSDDEDRAAEYGLQSLQSGGGDSLYFGLAGTNTTDGRAALPSFQADR
                     EEFLEYDVAKLINELGTPKKPVIGLISSLGLQGQFNPMTGQMGDPWPILTQLQELFTI
                     RTLTADVDHIDKEVDVLMLVHPKQLAPKTLYAIDQFVMRGGRLLLFVDPNAGADTSGQ
                     DPSNPMAGMLANHSSDLQPLLAAWGVDYDATKVIGDLERGLEVRTSMQAAPVRHIGIL
                     GLSHDDMNQKDVVSASLDKLNLATAGSLAQHPGAKTTFEPLLHSSASAAPIPAQRFNA
                     LTDPSTLRDGFKPTGIRYALAARITGPVESAFPQGPPPEQKAAAGPPAAHLTKSTAPA
                     NIVIIADTDLLMDYMWVQTREVFGQRVAQPFANNGDFVANILDNLSGSSALISVRGRA
                     TFSRPFERVEALRRQADDRLRGKALELQAELQQTESKLTDLQGKRNDQSSLVLSPEQE
                     AELKRFVAEKARVRKELRETQRGLDVDINRLNSWLKVINIAIVPLCVAVAGVIILSLR
                     RRRRTTAAGAA"
     gene            complement(2386..>2984)
                     /locus_tag="VGE92_14060"
     CDS             complement(2386..>2984)
                     /locus_tag="VGE92_14060"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY1315000.1"
                     /translation="QADLQPFFHWHPWLYLVLIPAISMRLWAEERNSGSIELLMTLPL
                     SLWQAVLGKFLAAWCFAGIALSLTFPIWITVNYLGSPDNGAIVAAYLGSLLMAGGFLS
                     IGMCLSAATRNQVVAFITAALVGFVFLLAGFPLVLDFVRGVVPQAVVDAIASLSFFTH
                     FEAISKGVIDLRDLVYFAALIGFWLAATALVLDMKKAE"
BASE COUNT          558 a          941 c          934 g          551 t
ORIGIN      
        1 tgtaacttcg gttctcgccg acccgccgcg cgaagttgcc ttcgccgacg ggcttgccga
       61 tgatcacggc atgcttcccg tcctgagcgc tgatgctgat ttccgcgccg ctcgcagtgg
      121 cggacgatgg atcatcgacg ccaatgaccg agaagttcgc cggattggag gtcttctcct
      181 cgacgatctt ggcatcgctc aaggcgagca gtaacttgcg cagcttcggc acgtcggccg
      241 gataatcacc gcgctccgcc accgtccaac ggcctgctcg gctgtgcagc gtcacgctgg
      301 gagttgcgct gccacgacgc acgctcaatt gcgtgaccgt attgatctcg cccgcaagcg
      361 cgggcagcaa ggcaacgccg atcgtctcgc gctgctgatt gcgttgcgcg ctcaggtaca
      421 aggcgccgcc gatggcgatg agcgctgcga tgagcagcgt caagaatcgt tgccggctca
      481 tgcggcgccc gctgcggtgg ttcggcgccg ccgccgcaga gacagaatga tcacgcccgc
      541 caccgccacg cacaacggca cgatcgcgat gttgatgacc ttgagccagg aattcagccg
      601 attgatatcg acgtcgagtc cacgctgcgt ctcgcgcagc tccttgcgca cgcgcgcttt
      661 ctcggcgacg aagcgcttca gttccgcctc ttgttcgggt gagagcacca gcgatgattg
      721 atcgttgcgt ttaccctgca aatcggtgag cttcgattcg gtctgctgca gctcggcctg
      781 cagctccagc gctttgccgc gtaagcggtc atccgcctgg cgccgcagcg cttcgacgcg
      841 ctcgaacggc cgcgagaatg tcgcgcgccc gcgcacgctg atcagagcgc tgctgccgct
      901 caggttgtcg aggatgttcg ccacgaaatc gccgttgttg gcaaatggct gcgcgacccg
      961 ttgaccgaag acctcgcgtg tttgcaccca catataatcc atcaacagat ccgtatcggc
     1021 gatgatgacg atattggcag gcgcagtgga tttggtcagg tgagccgccg gcggtcccgc
     1081 cgcagccttc tgctcgggcg gcggtccctg aggaaacgcc gattcgaccg gtccggtgat
     1141 gcgcgccgcc agcgcgtaac gaatcccggt gggtttgaag ccgtcgcgca acgtgctggg
     1201 atcggtgagg gcgttgaatc gctgcgcggg aatcggcgcc gcgctcgccg agctatgcag
     1261 cagcggctca aaagtcgtct tggcgccggg gtgctgcgca agcgagccgg cggtcgcgag
     1321 attgagtttg tcgagcgatg ccgaaacgac atccttttga ttcatgtcgt cgtggctgag
     1381 tcccaagatg ccgatatgcc gtaccggcgc cgcttgcatg ctggtgcgca cttcgagccc
     1441 gcgctccaag tcgccgatga ccttggtcgc gtcatagtcg acgccccagg cagcgagcag
     1501 cggctgcagg tccgatgagt gattggcgag cattccggcc atcggattgg acgggtcctg
     1561 gccgctcgtg tccgcacccg cattcggatc gacgaacaac aataaccggc cgccgcgcat
     1621 gacgaattga tcgatggcgt acagcgtttt cggcgccagc tgcttcgggt ggacgagcat
     1681 caacacgtcg acttccttgt cgatgtgatc gacgtcggcg gtcaaggttc ggatcgtgaa
     1741 cagctcctgc agctgggtga gtatcggcca tgggtcgccc atttgtccgg tcatcggatt
     1801 gaactgccct tgcagcccga gcgaactgat caggccgatc acgggctttt tgggggtgcc
     1861 gagttcattg atgagcttgg ccacgtcgta ttccaggaac tcttcgcgat cggcctggaa
     1921 gctcggcaac gccgcccgcc cgtccgtggt attggtgccc gcgagtccaa aataaagtga
     1981 atctccgccg ccggattgca aggattgcag cccgtattcg gcggcgcggt cctcgtcgtc
     2041 ggaaaacggc tgcggatcga tgatgcgcag gtgaatcttg ccgttggcgc gcgccgccag
     2101 ttcttcgaga aactcacgca cgcgggtggc gtacggcatc agcagcggcg cctgtttggc
     2161 ggcggcttcg cgcgagaagt aaaaatacag gttaaccggt tccttgagct cggccaatac
     2221 ttgctgcgtg ccgggactca aggtgtagag cctgttttgc gtcaagtcga tgcgcatgcc
     2281 gcgcaacccc aggttcgaca gcatcacgac gcccaagaac aatacggcga gcgcgatcag
     2341 cccgccgacc ccaaatcccc atctccgcca gcgactttcg ttcacctatt ccgccttctt
     2401 catgtcgagc accaaggccg tcgcggccaa ccagaagcca atcaaggcgg cgaaatacac
     2461 cagatcccgc agatcgatga cgcccttcga gatggcctcg aaatgtgtga aaaagcttaa
     2521 gctggcgatc gcatcgacca ccgcctgcgg cacgacgccg cgcacgaaat cgagcaccaa
     2581 cggaaatccc gccagcaaga aaacgaagcc gaccaacgcc gccgtgatga aggccaccac
     2641 ctggttgcgc gtcgccgccg acaggcacat gccgatcgac aggaagccgc ccgccatcag
     2701 caggctgccg aggtaggccg cgacgatcgc gccgttgtcc ggggacccga ggtagttcac
     2761 cgtgatccag atcggaaagg tgagcgacag cgcgatgccg gcaaagcacc aggcggcgag
     2821 aaatttgccg agcaccgcct gccacaggct gagcggcagc gtcatcagca gttcgatgct
     2881 gccgctgttg cgctcctccg cccagaggcg catggaaatg gcgggaatga gcacgagata
     2941 cagccacggg tgccagtgaa agaacggctg caggtcggct tgcc
//
LOCUS       DASWZT010000490         9337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_81426, whole genome shotgun sequence.
ACCESSION   DASWZT010000490 DASWZT010000000
VERSION     DASWZT010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9337
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81426"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VGE92_14065"
     CDS             <1..891
                     /locus_tag="VGE92_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY1315001.1"
                     /translation="AIPISATKSYGIYSGVVDAMMEWWYGHNAVGFFLTAGFLGMMYY
                     FVPKQAQRPIYSYRLSVVHFWALISVYMWAGPHHLHYTSLPDWVQSIGMAFSILLLAP
                     SWGGMINGMMTLSGAWYKLRTDPILKFMIVSLSFYGMATFEGPMMSIKTVNAMSHYTD
                     WGIAHVHSGALGWVAMMTIGSMYYLIPRLFNRTEMYSIKLIDVHFWTSTIGVVLYIAA
                     MWSAGVMQGLMWRAMNDDGTLTYSFVESVKATYPFYLVRLGGGLLFFLGMLIMAYNVW
                     MTVRNEKPLAIAVAPAAAGV"
     gene            893..1501
                     /gene="ccoO"
                     /locus_tag="VGE92_14070"
     CDS             893..1501
                     /gene="ccoO"
                     /locus_tag="VGE92_14070"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020292822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEY1315002.1"
                     /translation="MSHERIEKNVGLLTVLIILVISVGGLIEIVPLMLSAAKKQGITH
                     IVPRTALQQEGFDIYVREGCYLCHSQQIRPFRSETERYGPYSVAAESQYDHPFEFGSK
                     RTGPDLARVGGRYSDDWHRVHLHNPRDVVPESNMPGYPWLALNKIDATMTERKLRVLR
                     YLGTPYTDADIAGAAAAVADRTELDAVISYLQSLGLARPRGS"
     gene            1512..1706
                     /locus_tag="VGE92_14075"
     CDS             1512..1706
                     /locus_tag="VGE92_14075"
                     /inference="COORDINATES: protein motif:HMM:NF017366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HEY1315003.1"
                     /translation="MNAIILMSLRAAITVSLFALFIALIVWAWSPKRHKQFAEAANLV
                     FDETDRVAADPAPAKKEELR"
     gene            1706..2593
                     /gene="ccoP"
                     /locus_tag="VGE92_14080"
     CDS             1706..2593
                     /gene="ccoP"
                     /locus_tag="VGE92_14080"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEY1315004.1"
                     /translation="MSHTLSVVIAIVASLNVLGCAWLIWWTSRSRPDEVSKGEVIDHV
                     WDGDLQERNNPMPRWWLILFFLSIIFCFVYFLLYPALGTFGNVLGWSKASQYDQEMVA
                     AKKQYAPMYAAFAGRDIEDLAKDPKALVLGRSLFANNCIACHGSDARGAPGFPNLTDN
                     DWLHGGAPAQIVETITHGREGVMPALGPALGDQGVDEVADYVLSLSGRNEPPQLVAAG
                     KNRFLLCAACHGADGKGNQAIGAPNLTDDIWLYGGTDAAVRKSIKDGRHGQMPSHAWL
                     GEDKIKLLAAYVYSLSHAP"
     gene            2600..2908
                     /locus_tag="VGE92_14085"
     CDS             2600..2908
                     /locus_tag="VGE92_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315005.1"
                     /translation="MATPSPEGHSSDQRFLMDVGLVSWSSFLAACVASILFFAAVDPQ
                     LLRDAGPRIFDNLSREAGYALGFFFFWMLAASAAGLSVYMVRSAEWHDGHPDTERDSD
                     "
     gene            2908..4299
                     /gene="ccoG"
                     /locus_tag="VGE92_14090"
     CDS             2908..4299
                     /gene="ccoG"
                     /locus_tag="VGE92_14090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEY1315006.1"
                     /translation="MNAKLTTVAPQSSAPSFYEAAQKIYPREVRGRFATKRRWTVVVL
                     LGLFYGVAWISWNGQQLLLFDLPARKFHVFGLTLWPQDFVYLALLLIIAALSLFFVTA
                     LAGRVFCGYACPQTVWTEVFLWIEHWTEGTRSQRQKLDQRAWNLEKVLRKSSKQLLWL
                     SLAFYTGFTFVGYFMPIRSIGAGLVQLSLGPWAVFWILLYSLATYGNAGYLREQVCKY
                     MCPYARFQSAMFDKDTLVISYDELRGEPRGARRRGAPPAKNQGDCIDCTLCVQVCPTG
                     IDIRKGLQYECIACAACIDACDDVMDKVGSARGLIRYTTEHAMSGNRTRVARPRLFVY
                     GALLSFATGIIAYSLLTRTPLILDVIRDRNALYREVRGDWVENAYSIKVINEDEKAHH
                     YRLQAAGIAQLELVTPAGLIDVPPGSVANVPVRLQAPAASAQGVHPITLRITAQDAPS
                     IVRTEKTRFIGPQ"
     gene            4299..4451
                     /locus_tag="VGE92_14095"
     CDS             4299..4451
                     /locus_tag="VGE92_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315007.1"
                     /translation="MTSNTTAWYRQVWPWIIISLLSSAVIGSCLSAYLAVHTTDVVLE
                     HSDSSD"
     gene            4460..6916
                     /locus_tag="VGE92_14100"
     CDS             4460..6916
                     /locus_tag="VGE92_14100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY1315008.1"
                     /translation="MPALETCFHCGSSAAIGVRAIGVDGVERSFCCGGCAAATGLIFA
                     QGLERYYSFRTASDQAPRYEQRSWSIYDRDRALRSYTHVREDGDRELSLQIDGMHCAA
                     CAWLLDNSLRQLEGIRDIQVNAGSARAEIRFDPTQVSLSRVLQSIYVLGYEPRPLSFT
                     AGAPPWQTERRTALKRLAVAGFGMMQVMTYAVSLYAGALQGIAPDLEQLLRFVSLLVA
                     TPVVLYAAQPFFIAAWRGLRRRTLVMDLPVALSIAAAYLWSVWSTLRGRGAVYFDSAV
                     MFTFFLLLGRFVEMSLRNGFGLQQDAIARLLPESILRITATGEERVIPDELSAGDRVR
                     ILPGERVPADGEILCGRTEVDESMLTGESLPRVRAAGENLIAGTLNLANAVDMRITGV
                     GQDSTLAAVSRLLERARASRPHIAEAADAMAVWFVAGVLVLAVIAALYWLHVDAARAF
                     PTVLAVLVVTCPCALSLATPAALAAATTQLARMGLIVTRGRALETLARADCVVFDKTG
                     TLTFGQPVLTEIRLLRANASRERCLAVAAALERHSGHPIARAFAKQSPALDVSDTRSC
                     SGLGIEGRVGDRRYRIGRAEYVRELCATPVAAPQIPEGRSSIFLSDSAGLLAEFVLCD
                     ALRTDARDALQQLRRLGLEPLIASGDRTAVVTGIARQLDAAPGLGDLRPADKLNFVRS
                     LQAERRIVAMVGDGVNDAPVLAGADVSVAIGGGTDLAKVTADIVMLGEALAPLPAGIE
                     TARRCMRIIRQNLTWAVLYNAAAVPLAASGWLQPWMAAIGMSASSLLVTLNALRLLRP
                     PRPATAALDLPLQPPEVSPA"
     gene            6913..7092
                     /gene="ccoS"
                     /locus_tag="VGE92_14105"
     CDS             6913..7092
                     /gene="ccoS"
                     /locus_tag="VGE92_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEY1315009.1"
                     /translation="MNILYLLIPLGLVLVAIMVAAFFWATKSGQFDDLQTPAVQILLD
                     DDSKPMRHDRKSRDA"
     gene            7067..7825
                     /locus_tag="VGE92_14110"
     CDS             7067..7825
                     /locus_tag="VGE92_14110"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY1315010.1"
                     /translation="MIGSRAMHELALGSAFAAGVLGSTHCVAMCGGLAAALGTQRHGA
                     SRARPLLYQLGRVTSYGTAGAIVGSLGSVAGFAFEASQWSEVLRLGTALIVVLIGLNL
                     ALGSRRRGPWLRAPERWGALIWRRIAPLASKLPTQPALRALTLGLLWGWLPCGLVYSV
                     LLAAAFAGSALAGGATMIAFGLGTLPAMLGLSYASGRFTPPRASVARLLGSVIVACGL
                     WTATMPIAVLFGAHQHVHAPAHAHDTGYTHSTSV"
     gene            complement(7801..8688)
                     /locus_tag="VGE92_14115"
     CDS             complement(7801..8688)
                     /locus_tag="VGE92_14115"
                     /inference="COORDINATES: protein motif:HMM:NF009902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase family protein"
                     /protein_id="HEY1315011.1"
                     /translation="MAIVAGAITTSHVPAIGSAIAHGLQQEPYWRAFFDAYQGVHDWL
                     QKVRPDVVVVFYNDHGLNFFLDKMPTFAVGAADEYRYADEGWGIPAMFPPFRGHAQLS
                     WHLIESLVHDEFDLTTCQEMQVDHAFAVPMALLWPGVPSWPVMTVPIAINTVQHPLPS
                     ALRCLKLGQAVGRAIETFPQDLRVVIVGTGGLSHQLEGTRAGFINKDFDLMCMEKLVS
                     DPTALTRYSNAQLVDLAGSQGVELLMWVAARGALTGRVSSVHSNYHIPISNTAAGLMV
                     MENHTNALGRQRYTEVEWV"
     gene            complement(8692..9114)
                     /locus_tag="VGE92_14120"
     CDS             complement(8692..9114)
                     /locus_tag="VGE92_14120"
                     /EC_number="1.13.11.8"
                     /inference="COORDINATES: protein motif:HMM:NF009918.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 4,5-dioxygenase subunit alpha"
                     /protein_id="HEY1315012.1"
                     /translation="MVTPHCRAAIGLLESKKVRLFIEWREVLMDTQGIATPIFGGELA
                     QRGYALNKMCYSFNQATNRAAFIQDEQAYCSKFGLTEAQRRAVTHRNVLEMLQAGGNI
                     YYLAKLAGMLGLNVQDIGAQQTGMSVEAFKSKLAAAGK"
BASE COUNT         1624 a         2891 c         2902 g         1920 t
ORIGIN      
        1 gccataccca tcagcgcgac gaagtcctac ggcatctact ccggtgtcgt cgatgccatg
       61 atggaatggt ggtacggcca taacgccgtc ggctttttcc tgaccgccgg cttcctcggc
      121 atgatgtact acttcgtgcc caagcaggcg cagcgtccca tctactccta tcgtctctca
      181 gtggtgcatt tttgggcgct catttccgtg tacatgtggg ccggtccgca tcacctgcac
      241 tacacctcgc tgccggattg ggtgcagtcg atcggcatgg cgttctcaat cctgctgttg
      301 gcgccctcat gggggggcat gatcaacggc atgatgactc tgtcgggtgc ctggtacaaa
      361 ttgcgcaccg atccgatctt gaagttcatg atcgtgtccc tgtccttcta cggcatggcg
      421 accttcgaag gtcccatgat gtcgatcaag acagtgaacg ccatgtcgca ctacaccgac
      481 tggggtatcg cgcacgtgca ctccggcgcg ctcggctggg tggcgatgat gaccatcggc
      541 tcgatgtact acctgattcc gcgcctcttc aatcgcaccg agatgtacag catcaagctc
      601 atcgacgtgc atttctggac atcgacgatc ggtgtcgtgc tgtatatcgc cgccatgtgg
      661 agtgccggcg tgatgcaggg actgatgtgg cgcgccatga atgacgacgg cacgctgacc
      721 tacagtttcg tcgaaagcgt caaggcgacc tacccgtttt atctggtgcg cttgggcggc
      781 ggtcttttgt ttttcctggg aatgctgatc atggcctaca acgtctggat gactgttcgc
      841 aacgagaaac ctttggccat agcggtagcc cctgcggcgg cgggagtctg atatgagcca
      901 cgagagaatt gaaaagaatg tcggcctgct gacggtgctg ataatcctgg tcatcagtgt
      961 cggcggactc atcgagatcg tgcccctgat gctgagcgcg gcgaagaaac aggggatcac
     1021 gcatatcgtg ccgcgcaccg ccttgcagca ggaagggttc gatatctacg tgcgcgaggg
     1081 ctgctatctg tgccactcgc agcagattcg acccttccgc agcgaaaccg aacgctacgg
     1141 gccgtactcc gtggcagcgg aatcccaata cgatcatccg ttcgaattcg gttccaagcg
     1201 cactggcccg gatctggcgc gtgtcggcgg ccgctacagc gatgactggc accgggtgca
     1261 tctgcacaac cccagggacg tcgtgccgga gtcgaatatg ccggggtacc cctggctggc
     1321 gctgaacaaa atcgatgcca cgatgaccga gagaaaattg cgggtcctgc gctacttggg
     1381 aacgccttat accgacgcgg acatcgcggg cgctgcggcg gcggtcgcag accggactga
     1441 attggacgcg gtcatctcgt atctacaatc cttggggctc gcacgcccgc gcggttcgtg
     1501 agggcacgcc ggtgaacgca atcatactca tgagtctgcg cgcggcgatc acggtgtcgt
     1561 tgttcgcgct attcatcgcc ttgatcgtct gggcgtggag tccgaagcgg cacaagcaat
     1621 tcgccgaagc ggcaaatctg gtgttcgacg aaaccgatcg ggttgcggct gatcccgcgc
     1681 ccgcgaaaaa agaggagtta cgctgatgtc gcatacgctc agcgttgtga ttgccatcgt
     1741 cgccagcctg aacgtactcg gctgcgcttg gctgatctgg tggacgtcgc gcagccgacc
     1801 cgacgaggtc tccaagggcg aggtcatcga ccatgtatgg gacggcgatc tgcaagagcg
     1861 caacaatccc atgccgcgct ggtggctgat tctgttcttc ctctcgatca tcttctgctt
     1921 cgtctacttc ctgctgtatc ccgcgctcgg taccttcggc aacgtgcttg gatggagcaa
     1981 ggcctcccag tacgatcaag aaatggtcgc ggccaagaag caatacgcac ccatgtacgc
     2041 agccttcgcc ggccgcgaca tcgaggacct cgccaaggat cccaaggcgc tcgtgctcgg
     2101 ccgcagcctg tttgccaata actgcatcgc ctgccatgga tcggatgcgc gcggcgcgcc
     2161 tggatttccc aatctgaccg ataacgactg gctgcacggc ggagcgcccg cacaaatcgt
     2221 cgagaccatt acccatggtc gcgaaggcgt catgccggcg ctcggtcccg cgttgggcga
     2281 tcagggcgtc gatgaagtgg cggactatgt gctcagcttg agcgggcgca atgagccgcc
     2341 gcagctcgtg gccgccggga agaatcgttt ccttctctgc gccgcctgtc acggcgccga
     2401 cggcaagggc aatcaagcaa tcggcgcgcc gaatctgacc gatgatattt ggctctacgg
     2461 cggcaccgac gccgcggtgc gtaaatcgat caaggacgga cgtcacggcc aaatgccctc
     2521 tcatgcgtgg ttgggcgagg acaagatcaa gctgctggcg gcctatgtct acagcttgtc
     2581 gcacgcgccg tgagactcca tggcgacgcc aagccctgaa ggacacagca gcgatcaacg
     2641 gttcttgatg gacgtcgggc tggtgtcgtg gtcgtcattt ctcgccgcct gcgtggcgag
     2701 catactgttc ttcgcagcgg tcgatccgca gttgctgcgc gatgcgggcc cgcgaatttt
     2761 cgacaatctg agtcgcgaag cgggctacgc cctgggattt ttcttcttct ggatgctcgc
     2821 ggcgagcgcg gcggggctca gcgtgtacat ggtccgcagc gccgagtggc acgacggcca
     2881 cccggatacc gagcgcgact cagactgatg aacgcgaagc tcacaacggt tgcgccgcaa
     2941 tcctccgcgc caagcttcta cgaggctgcg cagaagattt atccgcgtga ggtgcgtgga
     3001 cgattcgcga ccaagcgccg ctggacggtg gttgtgttgt tggggttgtt ctatggcgtt
     3061 gcctggatca gctggaacgg gcagcagttg ctgctgttcg atctgccggc gcgcaaattt
     3121 cacgtgttcg gcttgacgct gtggccgcag gatttcgtgt atctcgcgct gctgctgatc
     3181 atagccgcgc tgtcgctgtt ttttgtcacg gccctggcgg gccgcgtatt ctgcggctac
     3241 gcatgcccgc aaacggtttg gaccgaagtg ttcctgtgga ttgaacactg gaccgagggc
     3301 acccgctcgc agcgccagaa acttgatcag cgtgcgtgga acctggagaa ggtgctgcgc
     3361 aagagcagca agcagctcct gtggctgtcg ctcgcttttt ataccggctt cacgttcgtc
     3421 ggttacttca tgccgatacg ctcgatcggc gccgggctcg tgcagctatc gctcggtccc
     3481 tgggcggtgt tctggatttt gttgtactca ctcgcaacct acggtaatgc cgggtacctg
     3541 cgcgagcagg tgtgcaagta catgtgtccc tacgcgcgtt tccaaagcgc catgttcgac
     3601 aaggatacgc tggtcatttc ctacgatgaa ctgcgcggcg agccgcgcgg cgcacgccgc
     3661 cgcggagcgc cgccggcgaa aaaccaaggc gactgcatcg attgcacgct ctgcgtgcag
     3721 gtctgtccca ccggcatcga tatccgcaag ggcttgcagt acgagtgcat cgcttgcgca
     3781 gcctgcatcg acgcctgcga tgacgtcatg gacaaggtcg gctctgcgcg cggcctgatc
     3841 cgttacacca cggagcatgc catgtcaggc aatcggaccc gcgtggcacg cccgcggctg
     3901 ttcgtctacg gcgcgctgct cagcttcgcg accggcatca ttgcctactc gttattgact
     3961 cgcacccccc tgattctcga cgtgattcgg gaccgtaacg ccttgtatcg ggaggtacgc
     4021 ggcgattggg tggagaacgc ctatagtatc aaggtgatca acgaagacga gaaggcccac
     4081 cattaccgcc tgcaggcagc gggcatcgcg cagctcgaac tggtgactcc cgcgggtctg
     4141 atcgatgtgc cgccgggcag cgtggccaat gtgccggtcc ggttgcaagc ccccgcggcg
     4201 agtgcgcagg gcgtgcatcc gattacactt cgaatcaccg cgcaggatgc cccgagcatt
     4261 gtccgtaccg agaaaacccg cttcattgga ccgcaataat gacaagcaac accaccgcct
     4321 ggtatcgcca agtctggccc tggatcatca tcagtctgct atcgagcgcc gtgatcggca
     4381 gctgcctcag cgcctatctc gccgtgcaca ccaccgatgt ggtgctcgag cacagcgatt
     4441 catccgacta ggccgcggcg tgcctgcact cgaaacctgt tttcattgcg gcagcagcgc
     4501 tgcgatcggc gtgcgtgcga tcggcgtcga tggcgtggag cgttcatttt gctgtggcgg
     4561 ctgcgcggcg gcgaccggtc tgatttttgc acaggggctg gagcgctact actcgtttcg
     4621 tactgcatcc gatcaagcgc cccgctatga gcaacgtagc tggtcgatct acgatcgaga
     4681 ccgcgcattg cgcagctaca cccacgtccg cgaggacggc gatcgcgaac tgagcctgca
     4741 gatcgacggc atgcactgcg ccgcctgcgc ctggctgctc gataatagcc tgcggcagct
     4801 cgagggcatc cgcgacatcc aggtgaatgc cggcagcgcc cgcgcagaga ttcgattcga
     4861 cccgacgcag gtgtctctaa gtcgggtgct gcagagcatt tatgtgctgg gttacgagcc
     4921 gcggcccttg agtttcactg ccggtgcgcc tccttggcag accgaacgac gcactgcact
     4981 caagcgtctc gccgtggcgg gtttcgggat gatgcaagtc atgacctatg ccgtgtcttt
     5041 gtacgccggc gcgttgcagg gaatcgcccc ggatttagaa cagctgctgc gattcgtcag
     5101 cctgctggta gccactcccg tggtgctgta cgcggcgcaa ccgttcttca ttgccgcatg
     5161 gcgcggtctg cgcaggcgta ctttggtcat ggatctgccg gtggcgctgt ccattgccgc
     5221 tgcgtatctc tggagcgtgt ggtcgacgct gcgcggacgc ggcgccgtgt acttcgattc
     5281 ggcggtcatg ttcacgttct tcctgctgct cggtcgcttc gtggaaatgt ccctgcgcaa
     5341 tggctttggg ctgcagcagg acgccatcgc gcgcttgctg ccggagagca ttttgcgcat
     5401 cactgcgacg ggagaggagc gcgtcattcc agatgagctc tccgcgggcg atcgggtgcg
     5461 gattctgccc ggcgagcgcg ttccggcaga tggtgaaata ctctgcggtc gcaccgaagt
     5521 cgacgaatcc atgctgaccg gcgaatcgct gccgcgcgtg cgcgcagcgg gggagaacct
     5581 gatcgcggga accctcaacc tggcaaatgc cgtggacatg aggataaccg gcgtcggtca
     5641 agactcgacg ctcgcggccg tgtcgcgttt gctcgagcgt gcgcgcgcga gtcggccgca
     5701 cattgcggag gcggcggacg cgatggctgt ctggttcgtc gcgggcgtgc ttgtgcttgc
     5761 cgtaatcgcg gcgctgtatt ggctccatgt ggatgccgcg cgcgcctttc ccaccgtgct
     5821 cgcggtgctc gtggtcacct gcccgtgcgc gttgtcgctc gccacaccgg cggcgctggc
     5881 ggcggccacg actcaattgg caaggatggg actcattgtc acgcgcggcc gggcgctcga
     5941 gacgcttgcc cgcgcggact gtgtagtctt cgacaagacc ggtacgctca cctttgggca
     6001 acccgtgctg acggagatcc gactgctgcg cgccaacgcc agtcgcgagc gttgcctcgc
     6061 cgtcgccgcc gcgcttgagc gccactccgg tcaccccatc gcgcgcgcgt ttgccaagca
     6121 atcgccggca ctcgacgtca gcgataccag atcctgctca ggattgggca tcgaaggccg
     6181 cgtcggtgac cgacgctatc gaatcggtcg tgcagagtac gtccgggaac tatgcgcgac
     6241 gccggtcgcc gcgccgcaaa taccggaggg ccgcagcagc atctttctca gcgatagcgc
     6301 agggttgttg gcggagttcg tgttgtgcga tgccttgcgc acggatgcgc gcgacgccct
     6361 gcagcagctg cggcggctcg ggctagaacc gctgatcgcc agtggcgatc gaacggcagt
     6421 cgtgaccggg atcgcgcggc aattggacgc agcgccgggc ctcggagact tacgcccggc
     6481 cgacaagctg aactttgtgc gctcgctgca ggccgaacgg cgcatcgtcg cgatggtggg
     6541 cgatggtgtc aatgacgccc cggtcctcgc cggtgccgat gtgtcggtgg ccatcggcgg
     6601 cggcactgat ctggccaagg tcactgccga catcgtcatg ctgggcgaag cgcttgcgcc
     6661 gcttcccgcc ggtatcgaga ccgcgcgccg ctgcatgcgc atcatccggc aaaacctcac
     6721 ctgggcggtg ctctataacg cggccgcggt gccgctggcg gcgagcggct ggctgcagcc
     6781 ctggatggcg gcaatcggta tgtccgccag ctcattgctg gtgacgctca atgccctgcg
     6841 cctgctgcga ccgccgcgcc ccgccacggc ggcgttggac ctgcctctgc agccgcccga
     6901 ggtgtcgccg gcgtgaacat tctgtacctc ttgataccgt tgggattggt tttggtcgct
     6961 atcatggttg cggctttttt ctgggccaca aaaagcggcc agttcgacga tctacaaacg
     7021 ccggcagtgc agattctgct cgacgatgac tcgaagccca tgcgccatga tcggaagtcg
     7081 cgcgatgcat gaactggcgc tcggcagtgc ctttgccgcg ggggtgctgg ggagcacgca
     7141 ttgcgtggcg atgtgcggag gtcttgcagc cgcgctcgga acgcagcggc acggcgcatc
     7201 gcgcgcccgg ccgttgctgt atcagctcgg acgagtgacc agttacggca ccgccggcgc
     7261 cattgtcggc tccttgggct cggtcgccgg ctttgcattc gaagcatcgc aatggagcga
     7321 agttctgcgc ttgggtaccg cgctcatcgt ggtgctgatt ggcctcaacc tggcgctggg
     7381 ctcgcgtagg cgcggaccct ggctgcgcgc gcccgagcgc tggggcgcgc tcatctggcg
     7441 gcgaatcgcg ccgcttgcct cgaaattgcc gacgcaacct gccctgcgcg cgctgacgtt
     7501 gggcttgcta tggggctggc tgccgtgcgg cctggtctac tcggtactgc tggccgccgc
     7561 gttcgccggc agcgccttgg ctggcggcgc caccatgatt gcattcggtc tcggcactct
     7621 gccggccatg ctgggattga gctatgcgag cggccgcttc acgccgccgc gcgcttccgt
     7681 ggcgcgactg ctgggatcgg tcatcgtcgc ctgcggcctg tggaccgcca caatgcccat
     7741 tgccgtcctc tttggcgctc atcaacacgt tcacgcgccg gcgcacgccc acgacacggg
     7801 ttacacccac tcaacttcgg tgtagcgttg ccttccgagc gcatttgtgt ggttctccat
     7861 caccatgagg ccggctgccg tattggagat tgggatgtga taattgctgt ggacactcga
     7921 cacccgcccc gtcaaagccc cacgggcggc cacccacatg aggagctcaa ccccttgaga
     7981 tcctgcgaga tctaccagct gcgcattcga ataccgcgtc aacgcggtcg gatcgctcac
     8041 gagcttctcc atacacatca aatcgaaatc cttgttgatg aatcccgcgc gcgtgccctc
     8101 aagctggtgc gacagtcctc ccgttcccac aatcacgacg cgcaggtctt gtggaaatgt
     8161 ctcgatcgct cgccccaccg cttgacccag cttcaaacac cgcaaggccg aaggcagcgg
     8221 gtgctgtacc gtattgatgg caatcggcac cgtcatgacc ggccacgaag ggacgccggg
     8281 ccacaataac gccatcggca cggcaaaggc atgatcgact tgcatctcct ggcacgttgt
     8341 caagtcgaat tcatcgtgta ccagcgattc gatcagatgc caagaaagct gcgcgtgtcc
     8401 gcgaaacggc gggaacatcg cgggaatacc ccacccctcg tccgcatacc gatactcatc
     8461 ggctgcgccg actgcaaacg tcggcatctt atccaggaag aaattcaatc catggtcatt
     8521 gtagaaaacg acgacgacat cgggacgcac cttttggagc cagtcgtgca caccctggta
     8581 cgcatcgaag aacgctctcc aatagggctc ttgttgcagt ccatgggcga tggcactgcc
     8641 gatggcgggc acatgtgacg tggtgatggc gccggccacg attgccatga tctattttcc
     8701 cgcagctgcc agtttggatt tgaacgcctc aacactcatg ccggtttgtt gtgcgccgat
     8761 atcttgaacg ttcaggccca gcatgccggc gagcttggct aagtaataaa tattgccgcc
     8821 cgcctgcagc atctcgagca cattgcgatg cgttacggcc ctgcgctggg cttcggtcag
     8881 gccaaatttg ctgcagtaag cctgctcatc ctggataaat gcggcgcgat ttgtcgcttg
     8941 attgaacgaa taacacatct tgttcaaggc gtagccacgc tgtgccagct cgccgccaaa
     9001 tatgggtgtt gcgattccct gcgtatccat aagtacctcg cgccactcga tgaagagccg
     9061 caccttttta ctttctagca ggccaatcgc tgctcggcag tgcggggtta ccaccgcctg
     9121 ctgcggcttt tacgtacctg cggcaaagca acaggtgtag catctgaatt gatcttcatt
     9181 cgggcgcaca tgctatgaaa atacccgtgg gactgtgcac cccgatggcg atcgctgggt
     9241 aggtcatgac acgggccgca atgatccgca ctatcacctc gatcatccat gggaacacgg
     9301 tcatttcccg ggcgccatgg ggcccggcca cgtgtgg
//
LOCUS       DASWZT010000491         8697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_61928, whole genome shotgun sequence.
ACCESSION   DASWZT010000491 DASWZT010000000
VERSION     DASWZT010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8697
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61928"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="VGE92_14125"
     CDS             complement(<1..926)
                     /locus_tag="VGE92_14125"
                     /inference="COORDINATES: protein motif:HMM:NF018877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HEY1315013.1"
                     /translation="MSALDALHAHLEAWLIEAAYPLWAHIGIDARNGGFVEAVDQSGN
                     PLAQPRRARIHPRQVYAFAQAGALGWRGDTATIVSRGMDYFTRHYQRPDGLIRALADA
                     DGAPLDNSAQLYDQAFALLGYAAAATAQGARAEYESRALHLDQAIEARFGANGGTFYS
                     LEQPSNYREPNPHMHLLEAYLAWAEIGLAPRWADGVRGLVDVALSRFTRKDSGAIGES
                     YLASGAPSPGEAGRRIEPGHQFEWAWLLLRCQPWYHAPLRGAALQLISIGEQFGVRGG
                     VAINALHDDFTIADGNARFWPQTERLKAALLAA"
     gene            complement(923..2158)
                     /locus_tag="VGE92_14130"
     CDS             complement(923..2158)
                     /locus_tag="VGE92_14130"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY1315014.1"
                     /translation="MRKTTRKNSPADTPPDIAGTAGPQLSGTNLARAGDHNQRVTLHA
                     VRVNGPVTRTELVLKTGLTPAAIANITNRLLRDRLILRAGRLHGARGQPATKFVINPD
                     SCFSIGLNVDRDHVTLVALDFVGKVRARASREIHFAKPSSVRLFFQRSIGRLLSEAGI
                     ARNRLVGVGVAFPDDIARAHLPDQPADYAAWAAVRVDDLIRDVLGIPVFIENDAAAAA
                     IGEMQFGSGHRYRSFFYLLVTAALGGGLVADGSYFRGANGRSGEIGWLRARSRTGEES
                     QLQNIVSLSCLYSRLAEQGYRVATPHGLTRLEPRARQIVDRWIHESTEALVDSFVAIN
                     CLINPEAILIGGRLPAALVDRLAISLNQRMASFAAQIPAIAPVARALTSDDAPAVGAA
                     ILPFSYRLLPTRFALLKAP"
     gene            complement(2184..3086)
                     /locus_tag="VGE92_14135"
     CDS             complement(2184..3086)
                     /locus_tag="VGE92_14135"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY1315015.1"
                     /translation="MSSKPLLGGIELGGTKCVCLIGTGPDDVRVRLSIPTGADANATL
                     SRIEAVFRDWKIEHGPIDALGIASFGPLELDRTSSRYGYITSTSKPGWRNTDLARRLA
                     KLFPVPVGFDTDVNGAALAEGRWGAAKHLADFAYVTVGTGVGVGLVVDGRPAYGFGHS
                     ELGHIRVARKGGDAWQGACTYHGDCVEGLASGAAIAARAGLPPAEIPRDSPVWELVAH
                     ALAQLLHTIVLATAPRRILIGGGVVSGRPELLGCIRRQLVDSLNGYLKLDDLAGGIDG
                     YAVPPGLGSLAGPLGALALAADTI"
     gene            complement(3086..4585)
                     /locus_tag="VGE92_14140"
     CDS             complement(3086..4585)
                     /locus_tag="VGE92_14140"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEY1315016.1"
                     /translation="MTDPNQIKRIAIVGGGTAGWMAASILARSLPGSGCAITVIESPD
                     IGIVGVGEATIPPIIDMLRLLSINEADFVKHTQATYKLGIKFTDWPRAGHSYWHPFGS
                     FGAMINLRPFHHAWAKARNAGLAPRFNDFSLCAALGDAGKFRFPDSSAQAPVAGLRYA
                     LHFDAVLVAQYLRTYAERLGVIRMERIVTEAPLRADGFIDELKFADGTHLQADLYIDC
                     SGFRGVLIEGALSTGYLDWSHVLPCDRAVAMPTELLTKRPPFTEANARAAGWRWRIPL
                     QHRVGNGYVYSSAHVSDTQALEDLTSAVGAKPLAEPRFLKFVTGRRKLFWNRNCVALG
                     LASGFLEPLESTSIHLVMSGVYKLLEHFPNTAFDQSNINSYNGELIQEIERIRDFLVL
                     HYLTGRSDTPLWAYCQSMQLPDSLLQRIELYRRTGRIRVKPGELFSDLSWFYIFEGLG
                     ITPESHDPLMDVVPLGQLREILGSLAQSTAVAAQQAPSHDSYFAAANSP"
     gene            complement(4687..7683)
                     /locus_tag="VGE92_14145"
     CDS             complement(4687..7683)
                     /locus_tag="VGE92_14145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1315017.1"
                     /translation="MSLEASLTGHAAFAADAAQSTSQAATKSDDLQEIVVTGYRQSLQ
                     TALDAKRNSNLPIESVAAEDIGKMPDNNVAESLQRLPGVQIDRSGANGQGNSVLIDGL
                     RQNLVTLNGDVFLTGKEFYVSGEASGGGAGGNKQYGSLETIPSELVTGIDVIKNPNAA
                     ITEGGLGGTINLKTADPLLAPDGLSLGGLFRESDAQRQNSKTPDGTLVGTFKLNDRVA
                     MSASLTYSDLKTHTDEYQDANRGGWVISNSATPPYTGLLTAAKVGTIAQNYIVPQLGY
                     FTDVNDESKEKGASFDIAVKLTDSITSNLLWFYSREDETTIDYSDKAWFNGQDTSPLP
                     GLDPTQPYSIDGNGTVQNATFNALGAETATLYQKNISQANNFQWLTKFDDGGPLRGIF
                     DASFAHATSNLQAAQADVEHGYYATGGGVATAPAAPGCNNGQLCGPTGPGNHGYEFSY
                     ANGGTSGLPSISYLAPYADVLNNPAYTTFKSNWAWANLTDQKNFAVKAEMQWDPAFTE
                     IKTTITAGVRYAGRDIDQTFGRYLINGTLADGLVAGTNTGTPNPGFGPYLYYQDPGYG
                     NPSIPYSTAISNPALALTVNNFAAGKIVVKNPATSGITNPSTYLQQVWAGAGVPNNTE
                     QFFKDDLSSFDVTERTTAPYLMLDMGGPSSRIHANFGVRLVNTDLTINAGNANPHGAT
                     YYGTASWNGLESNVLPVTTKRNYTDVLPSFNLVLDVTDTQKVRFGAAKVVAPQDLYSL
                     GLGQSFNFTRGANDPVTGDARFKFNNGSTGNANLDPYRASQFVISYENYFAPGGVASI
                     GTFYKQVDNFVETENILTTVKDDFGGTAGNVSQPVNAGNGRIYGLEVGGQFNFGDTVA
                     WLQGVGAAANYTYSQSISQQPSSFSTTGPIPGVSRNALTGTLYYENHGFSVRGSYSYR
                     DKALNDSQVGSTFAFGTKVYEVFSAPYGQLDAQASYDFNKHVGVIVSVQNLTDEAQHT
                     YLQWPNQPFTYDDWGRRFFIGVKGKL"
     gene            8073..8198
                     /locus_tag="VGE92_14150"
     CDS             8073..8198
                     /locus_tag="VGE92_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315018.1"
                     /translation="MSPRVAIRIEAISPVEICAIARSNPASIKPSQKARSGAQQR"
     gene            complement(8508..>8697)
                     /locus_tag="VGE92_14155"
     CDS             complement(8508..>8697)
                     /locus_tag="VGE92_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315019.1"
                     /translation="SRTVPKYMMRARHSVCATVRNVNRRCRATMNARRSQRRAGSRPS
                     DEWRSLIAAPSVGANFAW"
BASE COUNT         1648 a         2622 c         2784 g         1643 t
ORIGIN      
        1 gcggcgagca atgccgcctt gaggcgctcg gtttgcggcc agaaacgcgc attgccatcc
       61 gcaatcgtaa agtcgtcgtg cagggcgttg atcgcgacgc cgccgcgtac cccgaactgc
      121 tcgccgatcg agatgagttg cagggcggcg ccgcgcaggg gcgcgtggta ccacggttgg
      181 caacgcagca gcaaccaggc ccattcaaat tggtgcccag gctcgatccg gcggccagct
      241 tcgcccgggg atggcgcgcc ggatgcaaga taggattcac caatggcgcc gctgtccttg
      301 cgcgtgaaac gagatagtgc cacatcgacc aggccgcgga cgccatccgc ccatctcggc
      361 gcgagtccga tctcggccca cgccaagtac gcctccaaca agtgcatgtg tggattcggt
      421 tctcgatagt tagatggctg ttcaagcgaa taaaaagtgc cgccgttcgc gccgaagcgc
      481 gcttcgatcg cctggtctag atgcagcgcg cggctctcgt actcggcgcg agcgccctgc
      541 gccgttgccg cggccgcata ccccagcagc gcgaacgcct ggtcatagag ctgcgcggaa
      601 ttatcgagcg gcgccccgtc ggcgtccgcc aacgcgcgaa tcagcccgtc cgggcgctgg
      661 taatggcgcg tgaaataatc catcccgcga ctcacgatcg tcgccgtatc gccgcgccag
      721 ccaagtgcgc cggcctgagc aaacgcgtat acctggcggg ggtgaattcg cgctcgtcgc
      781 ggttgtgcca gagggttgcc ggattggtcg accgcctcga cgaaaccacc gttgcgggca
      841 tcgatgccga tgtgcgccca cagcggatag gcggcttcga tcagccacgc ctcgaggtgc
      901 gcatgcaagg cgtcgagtgc actcatggcg ctttgaggag cgcgaatcgc gtcggcagca
      961 gccgataact gaagggcaga atggccgcac cgactgcggg agcatcgtcc gacgtcagag
     1021 ctcgagcgac cggcgcgatt gccgggattt gggctgcgaa cgaggccatg cgttggttca
     1081 atgagattgc caatcggtcg accaatgccg cgggtagccg cccaccgatg aggatcgcct
     1141 ccgggttgat caggcagttg atcgctacga acgagtctac cagggcctcg gtggattcgt
     1201 ggatccaacg atccacaatc tgccgcgcgc gaggctcgag gcgcgtcagt ccatgcggcg
     1261 tcgccacccg atagccctgc tcggcgagac ggctataaag gcaggacaac gagacgatgt
     1321 tctgcaattg gctttcctcg cccgtacggc tgcgtgcacg cagccatccg atttcgccgc
     1381 tgcggccgtt cgcgccgcga aaatagctgc cgtcggcgac caaaccgccg ccgagagccg
     1441 cggtcacgag caaatagaag aagctgcggt agcggtgccc cgagccaaac tgcatttcgc
     1501 cgatcgccgc cgccgcggcg tcgttctcga tgaacacggg aatcccgagc acgtcccgga
     1561 tcaggtcgtc gacgcgcacc gcagcccagg ccgcgtagtc ggccggttga tccggaagat
     1621 gggcgcgcgc gatgtcgtcg ggaaaggcga cgcctacgcc gaccagccgg ttgcgcgcga
     1681 tgccggcctc gctcagcagc cggccgatcg agcgctgaaa gaacaggcgc accgaggacg
     1741 gtttagcaaa atggatctcg cgcgaagccc gagcgcgcac tttgccgacg aaatcgagcg
     1801 ccaccagcgt gacgtgatcg cgatcgacgt tgagtccgat ggaaaaacag ctatcgggat
     1861 tgatcacgaa cttggtggca ggctggccgc gcgcgccgtg taggcgcccg gctcgcagga
     1921 tcagccggtc gcgcagcagc cgattcgtga tattggcgat tgccgcagga gtcaggccgg
     1981 ttttcaacac caactcggtg cgagtgaccg gaccattgac ccgcacggcg tgcagggtca
     2041 cgcgctggtt gtgatcaccc gcgcgcgcga ggttggtgcc ggacaattgc ggaccggccg
     2101 tgccggcaat atccggcggg gtgtccgccg ggctgttttt tcgcgtggtc ttgcgcaatt
     2161 tgcgggctgc aagcgtagcg ggatcaaatg gtatcggctg ccagcgccag cgcgcccaac
     2221 ggtcccgcca acgatccgag tcccggaggc acggcgtagc catcgatacc accggccaga
     2281 tcatcgagct tcaaatatcc attgaggctg tcgacgagct gccgtcgtat gcatccgagc
     2341 agctcgggcc gccccgaaac gacgccgccg ccgatcaaga tgcgccgcgg cgccgtcgcc
     2401 agcacgatgg tgtgcagcag ctgcgccagg gcatgcgcca ccagttccca taccgggcta
     2461 tcgcgcggga tttcggcggg cggcagaccg gcacgcgcgg caatggccgc gcccgaggcg
     2521 agaccttcga cgcaatcgcc gtgatacgtg catgcgcctt gccacgcatc gccgccttta
     2581 cgtgcgactc tgatatgccc gagctcggag tggccaaacc catacgccgg gcgtccgtcc
     2641 accaccaggc cgactcctac gcccgtgccg accgtgacat aggcaaaatc cgccaggtgc
     2701 ttggccgcgc cccagcgacc ctccgcgagc gccgcgccat tcacatcggt atcgaatccc
     2761 acgggcaccg gaaacagctt cgccaagcgc cgggcgagat cggtgttgcg ccatcccggc
     2821 tttgaagtcg atgtgatgta tccatagcgg gatgaagttc gatcgagctc gagcggacca
     2881 aaagaggcga tcccgagagc gtcgatcggg ccgtgctcga ttttccagtc gcgaaagacg
     2941 gcttcgattc gactcaaggt agcgtttgca tcggcgccgg tggggatcga cagccgcacc
     3001 cgaacgtcat ccggacccgt gccgatcaaa cacacgcatt tggttccgcc aagttcaatg
     3061 ccgccgagca gtggcttcga ggacatcacg gcgaattggc ggcggcaaaa tagctgtcgt
     3121 gggaaggcgc ttgctgcgcg gcgacagcgg tcgactgcgc cagcgagccg agaatttcgc
     3181 gcaattggcc gagcggcacc acgtccatca atggatcgtg gctctcaggg gttattccca
     3241 atccctcgaa gatgtaaaac cagctcaggt cgctgaataa ctcgccgggc ttgacacgga
     3301 tccgtccggt tcgccggtac agctcgattc gctgcagcaa gctgtcggga agctgcatgg
     3361 attggcaata ggcccaaagc ggcgtgtcgc tgcgtccggt caagtagtgc agcaccagaa
     3421 aatcgcggat gcgctcaatt tcctgtatca attcgccgtt gtaggaattg atgttcgact
     3481 gatcgaatgc ggtattggga aagtgctcga gcagtttgta aacgccgctc atgacgaggt
     3541 ggatgctggt cgactcgagc ggctcgagaa aaccggacgc gagtccgagc gccacgcaat
     3601 tgcgattcca gaatagcttg cgacgtccgg tcacgaactt gaggaaacgc ggctccgcca
     3661 gcggtttggc accgacagcg gaagtcaaat cctcgagtgc ctgggtatcg ctgacatgag
     3721 cgctcgaata cacgtaacca ttgccgacgc gatgctgcaa ggggatgcgc caacgccacc
     3781 cggcggcgcg cgcgttcgct tcggtgaacg gcggccgctt ggtcagcaat tcggtcggca
     3841 tggcgacggc acgatcgcaa ggcagcacgt ggctccagtc gagatagccc gtgctgagcg
     3901 cgccttcgat cagcacgccg cgaaagccgc tgcaatcgat atacaaatcg gcctgcaaat
     3961 gcgtgccgtc ggcgaacttc aattcgtcga tgaagccatc tgcgcgcagc ggtgcctcgg
     4021 tgacgatgcg ttccatccgg atcacgccca atcgttctgc atacgtccgc aagtactggg
     4081 cgaccaggac tgcatcgaag tgcagcgcgt agcgcaatcc cgccaccggc gcttgagctg
     4141 agctgtctgg aaatcggaat ttgccggcat cgccgagcgc agcacacaga ctgaaatcat
     4201 tgaaacgcgg tgcgagcccc gcattgcgag ccttggccca tgcatggtgg aagggacgca
     4261 gattgatcat cgcgccgaag ctgccgaagg gatgccagta agagtggccg gcgcgaggcc
     4321 aatccgtgaa cttgatgccg agcttgtagg ttgcctgcgt gtgtttgacg aaatcggctt
     4381 cgttgatact gagaagccgc agcatatcga tgatcggggg aatcgttgct tcgccgacgc
     4441 cgacgatgcc gatgtccggc gactcgatga ccgtgatcgc gcagccgcta cccggcagtg
     4501 agcgcgccaa gatgctggca gccatccaac cggcggtccc gccgccgacg atggcgatgc
     4561 gtttgatctg gttagggtcg gtcatgatcg gctcgcaagg cgagaatact cttcgcgtca
     4621 ttgcccagcg gcaagaaaaa aaggcaccgc cacccacagg gggatcggcg tggcggtgcc
     4681 cgggctttag agtttgccct tcactccgat gaagaagcgc cggccccaat cgtcataggt
     4741 aaaaggctga ttgggccact gcaagtaggt gtgctgggcc tcatccgtga ggttttgcac
     4801 cgatacgatg acgcccacat gcttgttgaa atcataactc gcctgcgcat cgagttgccc
     4861 gtaaggcgcc gagaacactt cgtagacctt ggtgccgaac gcgaacgtcg agccgacctg
     4921 cgagtcattg agggctttat cccgataaga atacgagccg cgcaccgaga agccatgatt
     4981 ctcgtagtac agcgttcccg tcagcgcatt cctggagaca ccggggatcg ggccggtggt
     5041 cgagaagctg ctcggttgct gcgagatcga ttgcgagtag gtgtagttcg ctgccgcgcc
     5101 gacgccttgc agccaggcca ccgtgtcgcc gaagttgaac tgtccgccga cttcgaggcc
     5161 gtagattcgg ccgttgccgg cattgacggg ctggctgaca ttgccggccg taccgccgaa
     5221 gtcatctttg accgtcgtca aaatattttc ggtttctacg aagttgtcca cctgcttgta
     5281 gaacgtgccg atgctggcga cgccgccggg cgcaaagtag ttctcgtatg agatcacgaa
     5341 ctgcgatgca cgatatgggt cgaggttggc attgccggtc gagccattat tgaacttgaa
     5401 gcgcgcatcg ccggtcacgg ggtcgttggc gccgcgtgtg aagttaaacg attgacccaa
     5461 tcccagcgaa tacagatcct gcggcgcgac cactttcgcg gcgccgaatc tcaccttctg
     5521 ggtgtccgtc acatcgagca ccaagttgaa cgacggcaaa acgtcggtgt aattgcgctt
     5581 ggtggtgacc gggagcacat tcgattccag gccattccag gaggccgtgc cgtaatacgt
     5641 cgcgccatgc gggttggcgt tgcccgcgtt gatcgtgaga tcggtgttga ccaggcgcac
     5701 gccgaagttc gcgtgaatcc ggctgctcgg gccgcccatg tcgagcatca ggtatggcgc
     5761 cgtggtgcgt tcagtcacat cgaacgaact caagtcgtcc ttgaagaact gctcggtgtt
     5821 gttcggaacg cccgcgcccg cccacacctg ctgcaagtaa gtcgacgggt tggtgatgcc
     5881 gctggtcgcg gggtttttca ccacgatttt gccggccgca aagttgttga cggtgagcgc
     5941 cagggcgggg ttcgatatcg ccgtggagta agggatgctg gggttgccgt agcccggatc
     6001 ctggtagtag aggtaaggtc caaagcccgg attgggtgtg ccggtgttgg tgccggcgac
     6061 caggccatcg gctaacgtgc cgttgatcaa gtaccggccg aatgtttggt cgatgtcgcg
     6121 gccggcatat ctcacacccg cggtaatcgt ggtcttgatt tccgtgaacg ccggatccca
     6181 ctgcatttcc gccttcactg caaagttctt ttgatcggtc agattggccc aggcccagtt
     6241 tgatttgaac gtggtgtaag ccggattatt caagacatcg gcatagggcg ccaggtagct
     6301 gatcgagggc agacccgagg tgccgccgtt cgcgtagctg aattcatagc cgtgattgcc
     6361 gggtccggtg gggccgcata actggccgtt attgcagccc ggcgctgcgg gcgccgttgc
     6421 cacgcccccg ccggtggcgt agtagccatg ctcgacgtcg gcctgcgcgg cctgcaggtt
     6481 cgaggtggcg tgcgcaaatg aagcgtcgaa gatgccgcgc agcgggccgc cgtcgtcgaa
     6541 cttggtcagc cattgaaagt tgttcgcttg cgagatgttc ttctgataga gggtcgcggt
     6601 ttcggcgccg agcgcgttga aggtcgcatt ttgcacggtg ccgttgccgt cgatcgaata
     6661 gggctgggtc gggtcgaggc cgggcagcgg gctcgtgtct tgaccgttga accacgcctt
     6721 gtccgagtaa tcgatggtgg tctcatcttc tcgcgagtag aaccacagca ggttgctggt
     6781 gatcgaatcg gtcagtttca ccgcgatatc gaaggaggca cccttttcct tggactcatc
     6841 gttcacgtcc gtgaaatagc cgagctgcgg aacgatgtag ttctgggcta tggttccgac
     6901 cttggcggcc gtgagcaagc cggtgtaggg aggggtggca gaattcgaaa tgacccagcc
     6961 gccgcgattg gcatcctggt attcatcggt atgcgtcttc aaatcggagt aggtgaggct
     7021 ggcactcatt gccacgcggt cgttgagctt gaacgttccg accaaggtgc catcaggagt
     7081 cttggagttc tggcgttgcg cgtcggattc gcgaaataac ccgccgagcg acaggccatc
     7141 gggtgccagg agcggatcgg cggttttcag attgatcgtg ccacccaggc cgccttcggt
     7201 gatggcggca ttcggattct tgatgacatc aatgcccgtc accagttcgc tcgggatggt
     7261 ctccaaggag ccatactgct tgttgccgcc ggcgccgccg cccgacgcct cgccggaaac
     7321 gtagaattcc ttgcccgtca gaaacacgtc gccgttcaaa gtcacgagat tttgccgcaa
     7381 gccatcgatc aggacggaat tgccctgacc gttggctccc gagcgatcaa tctgcacgcc
     7441 gggtaggcgc tgcagcgact cggcgacgtt gttgtcgggc atcttgccga tatcttcggc
     7501 ggccacggac tcaatcggca gattggagtt gcgcttggca tccaaggcgg tttgcagtga
     7561 ctggcggtag cccgtcacca cgatttcctg caaatcgtcg gattttgtcg cagcctgcga
     7621 ggtgctttgt gctgcgtcgg cggcgaaggc cgcatgcccg gtcagagagg cttcgagtga
     7681 caccgctgtc attattgcta aacgagcagc ctggcgcgcc cgcttttctc tacgatgcga
     7741 catggtacga ggtctcccag tagtttaact tttcctgcca tcggcgaggt tcgactcgag
     7801 accgacgtca tgcttgggca tgcttctgtc ggctgcgagt ccctctgctc ttccccacca
     7861 cccgggtcag tgcccgatgt gatctgcagc gaaggctaac tccgattcgc ggctggagtc
     7921 aacgattaaa tattttcttt gtctttaatc gcggccgcac gtggcctcgc cggggatgga
     7981 ttcacatatt gcactgcgcg accctatgcg gcgcggcgac ccgccggcga gccgcgccga
     8041 cgccgccgcg tagttcgcaa ttcctggatc gggtgagccc gcgggttgca attcgcatcg
     8101 aggcaatttc cccggtcgag atctgcgcca tcgcgcgcag caacccggcc tcgatcaagc
     8161 catcgcagaa ggcgcgctcg ggtgcgcagc agcggtgacg ccgagttgcg gtgagccgct
     8221 cgcgcacggc gcgaggccgg cgcggtgcgc gacctttcga ttcgcgcaaa tggcctgaat
     8281 ataaggatga aacatcaagg cgagcgatgt gcgcgcgtcg cttcgcaacg ccaggcatgg
     8341 cacgtgacga gcgcagggac aacccgtgcc agctgactca ttcgaggcac acaaggtgac
     8401 gcattcatca gcaccgccgg cgcgatggat ccgctgacat cgaagacacg cgctcggcaa
     8461 acccatggcg ctgctcagcg gcgcgacatg agtgcgtgaa cacgcactca ccaagcgaag
     8521 tttgcgccga cggacggcgc agcgatgagc gaacgccatt catcgctcgg acgcgaacct
     8581 gcgcggcgct gcgagcgccg cgcgttcatc gtcgctcgac agcggcgatt gacgttgcgc
     8641 acggtcgcgc aaacggagtg ccgagcacgc atcatgtatt tcgggacagt gcgagaa
//
LOCUS       DASWZT010000492         1883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_86150, whole genome shotgun sequence.
ACCESSION   DASWZT010000492 DASWZT010000000
VERSION     DASWZT010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86150"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="VGE92_14160"
     CDS             complement(<1..775)
                     /locus_tag="VGE92_14160"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEY1315020.1"
                     /translation="MPQNKSPRSDSGAAWQAIVSGNAELKLSTVPGGAGSALQMDFDF
                     KGAGGFVVARRADKRSMPEEFALSFRLRGRGAVNNLELKLVDASGLNVWRYVHKDLKP
                     PPRWKRMTVESSDIDFAWGPASGGRIQTLGSMEFAIVAGEGGTGTLWISDIRIEDRTP
                     QESPTAAASSAVPQFAATAALQGTGWKPRSDDRRPWIAIDFIEPRTIGGVIVDWLECA
                     PASGFRIRTSNSGHRWKTVYTASEAGGKRSYVYLPGTKTR"
     gene            complement(880..>1883)
                     /locus_tag="VGE92_14165"
     CDS             complement(880..>1883)
                     /locus_tag="VGE92_14165"
                     /inference="COORDINATES: protein motif:HMM:NF014800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315021.1"
                     /translation="HDLESATALNTRIRRVLREEQIYRIDHFLGKETVQSIMALRFAN
                     GLFEPIWNRNFIDHVQIDVPEKLTLGKRAGFYEHTGAYRDMVVTHLFQILGFMAMEAP
                     TSLEPNPISEEKNKVFRSMLSIKPADVIRGQYTGYRTEDGVSSDSDTETFIALRCYID
                     NWRWAGVPFYLRTGKRMAQGQRIISIAFKEPPKSMFPAGSGVSAQGPDHLTFDLADAA
                     KMSLSFYGKRPGPGMRLDKLSMQFAMHDTGHIGDVLEAYERLILDAMRGDHTLFTTAE
                     GIERLWEVSTTLLESPPPVRFYEPGSWGPNAIHQLVAPHAWRLPFERVWRDPNTVGS"
BASE COUNT          356 a          580 c          575 g          372 t
ORIGIN      
        1 atcgggtctt ggtcccgggc agatacacgt agctgcgctt gccgccggcc tcggaggcgg
       61 tatacacggt cttccaacga tggccgctat tcgaggtgcg aattcgaaag ccgctcgccg
      121 gtgcgcattc gagccagtcg acgatcacgc cgccgatggt tcgcggctcg atgaaatcga
      181 tcgcaatcca gggtcgtcgg tcgtcggacc ggggcttcca gccggtgccc tgcagcgcag
      241 ccgttgctgc gaattgcggc acggcgctcg acgcggcggc tgttggcgac tcctgggggg
      301 tgcgatcttc gatcctgata tcggaaatcc acaacgtgcc ggtgccgccc tcacccgcga
      361 cgattgcaaa ctccatggaa ccgagcgtct ggatgcggcc accgctcgcg ggaccccagg
      421 caaaatcgat gtcggagctc tccaccgtca tgcgcttcca acgcggcggc ggcttgagat
      481 ccttgtgcac gtagcgccat acgttcagtc cggaggcgtc gacgagcttg agctccagat
      541 tgttgacggc accgcgcccg cgcaatcgaa aacttagcgc gaactcctcc ggcatgctgc
      601 gcttgtccgc gcggcgcgcg accacgaagc cgcccgctcc tttgaaatcg aaatccattt
      661 gcagtgcaga accggcaccc cccggcaccg tcgacaactt cagttcggca ttgccggaga
      721 cgatggcctg ccaagcggcg cccgagtcgg atcgagggga tttgttctga ggcatcgata
      781 ccccgcgttg gttgacgatc ggccggctgt ccggctcagg ctattttaga ttagattaat
      841 tctataacta gttgttttgc tgaggaatgt aagaggagat cagctaccca ccgtattggg
      901 gtcgcgccag acccgctcga agggcaggcg ccacgcgtgc ggcgcgacga gttggtgaat
      961 tgcgttcgga ccccacgatc ctggctcgta aaagcgcacg ggcggaggcg actcgagcaa
     1021 ggtggtcgaa acctcccaca gccgctcgat accttccgcg gtcgtgaaca gcgtgtgatc
     1081 gccccgcatc gcgtccaaaa tcaaccgctc atacgcctcg agcacatcgc cgatgtggcc
     1141 ggtatcgtgc atggcgaact gcatgctgag tttatccaat cgcatgcccg gtcccgggcg
     1201 cttgccgtag aaggacagtg acatcttcgc ggcatcggcc aaatcgaacg tcaggtgatc
     1261 cggcccctgt gcgctgacgc cggagccggc agggaacatg ctcttgggcg gctctttgaa
     1321 cgcgatggaa atgattcgct gcccctgcgc catccgctta cccgtgcgca ggtaaaacgg
     1381 cacgccggcc catcgccagt tgtcgatata gcagcgcagc gcgatgaagg tttcggtatc
     1441 cgagtcgctc gagaccccgt cctccgtccg gtacccggtg tactgcccgc gaatcacatc
     1501 ggcaggcttg atcgacaaca tgctgcgaaa gactttgttc ttttcttcgc tgatgggatt
     1561 gggctccagg gaagtcggtg cttccatcgc catgaatccg agaatctgga acaagtgagt
     1621 caccaccatg tcgcgataag cgccggtgtg ttcgtagaag cccgcacgct tgccgagggt
     1681 caacttctca ggcacgtcga tctgcacatg atcgatgaag ttgcggttcc agatcggctc
     1741 gaacaagccg ttggcgaagc gcaacgccat gatgctctgg acggtctcct tgccgaggaa
     1801 atggtcgatc cgatagatct gttcctcgcg cagcacgcgg cggatacggg tgttcagcgc
     1861 cgtggcggat tccagatcgt gac
//
LOCUS       DASWZT010000493         1936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_37908, whole genome shotgun sequence.
ACCESSION   DASWZT010000493 DASWZT010000000
VERSION     DASWZT010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37908"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..>1936
                     /locus_tag="VGE92_14170"
     CDS             139..>1936
                     /locus_tag="VGE92_14170"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY1315022.1"
                     /translation="MLDGLATYVETWAEGLYCSVLLTDPAGRLLRPGAAPSLPVAYVA
                     AINPVPIATGRGSCGTAAALCEMVVVEDVERSELWRDYAQLALAHGLRACWSVPILAD
                     TRELLGTLALYYRIPRKPTSHEIELIQFAAALAAFVIQRHRSDDRLRTSQARLRAAVV
                     GADIGLWDCDPAGDGVWFDDWCQRLDIHPCDGPNWEAQWIAQIHPDDLERYKLSDREC
                     DLGHADQYVSEYRVRTRAGRWRWLYERGSVAARDAAGRPLHYVGLCIDIDDRKNGELA
                     LRQSDERFRTFARLVQGYVFEGSLQADLNVEFTWADDAFAELFGCERAEVNRRGWHSF
                     VDPRDRAAAAQCLATIAAGCNAHIELRIESAVGKRRWLRMAGEPVRDLVSGSVTGLIG
                     MAEDITDRKVLMEQMFASVNREQRRIGNDLHDELGQVLTGASLLLRGCHTSALRGEPV
                     TPGEIDRVLELLAGAIENTRSLAHGLAPGMLDCGGLTSALENLAAQARRWSGLEIALS
                     LSPGDLGTLDASTSDHLYRMVQEAITNVARHAHARRAEISTRLEDGALIIEVSDDGIG
                     IPASVSNGFGLRTIRYRAQAIGAEFAINAARPR"
BASE COUNT          329 a          639 c          639 g          329 t
ORIGIN      
        1 cgtatagctg gatcggactg ggcctgcgca gtcgacgcga tcttaaagaa tgcacccgag
       61 tctgctctgc cgcgcggatc gctcggcgcc caggtacgcc tcctcgcgct gctcggcgaa
      121 gctgccccac tcacggtatt gctcgatgga ctcgccacgt atgtggaaac ttgggccgaa
      181 ggcctttact gcagcgtgct gctcacggat ccggccggcc gcctgctgcg gcccggtgcg
      241 gctccgagcc tgccggtagc ctacgttgcg gccatcaatc cggtgccgat cgcgacgggg
      301 cgcggctcct gcggtacggc tgcggccctt tgcgagatgg tggtcgtcga ggacgtggag
      361 cgctcggagc tgtggcgcga ctacgcgcag ctcgccctcg cgcacggact gcgcgcctgt
      421 tggtcggtgc ccattctcgc cgacacgcgc gaacttctcg gtacgctcgc gctgtactac
      481 cgaatcccac gaaaaccgac gagccatgag attgagctga tccaattcgc cgccgcgtta
      541 gccgctttcg tgattcagcg ccatcgcagc gatgaccggc tgagaaccag ccaagcgcgc
      601 ctgcgcgcgg ccgtggtcgg cgccgacatc ggactatggg actgcgaccc tgccggcgac
      661 ggcgtgtggt tcgacgactg gtgccagcgg ctcgacatcc acccctgcga cggccccaat
      721 tgggaggccc aatggattgc gcagatccac ccggacgatc tcgagcgcta caaattatcg
      781 gaccgagagt gtgacctcgg ccacgcggat caatacgtca gcgagtaccg ggttcgaacc
      841 cgcgccggcc gctggcgttg gctctacgag cgcggcagcg tggccgctcg ggacgctgcc
      901 ggacggccgc tgcactatgt cgggctgtgt atcgatatcg atgatcgcaa gaacggcgaa
      961 ctggcgctgc gccaatccga cgagcgcttc cggacattcg cgcggctggt gcaaggctac
     1021 gtgttcgaag gcagcctgca ggccgatctc aacgtcgaat ttacctgggc ggacgatgca
     1081 ttcgcggagc tcttcggctg cgagcgcgcc gaggtcaatc ggcgaggctg gcacagtttc
     1141 gtcgatccgc gcgaccgggc ggcggctgcc caatgcttgg cgacgatcgc cgccggctgc
     1201 aacgcccaca tcgaactgcg catcgagagt gccgtcggca agcgacgctg gctgcgaatg
     1261 gccggggaac cggtgcggga tctggtcagc ggatccgtga ccgggctcat cggcatggcc
     1321 gaagacatta cggatcgcaa agtcctgatg gagcaaatgt tcgcctcggt caatcgcgag
     1381 caaagacgaa tcggcaatga tctgcacgac gaactcggcc aagtattgac cggcgcgagc
     1441 ctgctgctgc gcggctgcca cacgagcgcg ctgcgtggcg agcccgtgac gcccggcgaa
     1501 atcgaccggg tgctcgaact gctggccggt gcaatcgaga acacgcgttc gctggcccat
     1561 ggcctcgctc cgggcatgct cgattgcggc ggcctcacct cggcgctgga gaacctcgcc
     1621 gctcaagcac ggcgctggtc tggtctggag atcgcattgt ccttgagccc cggggatctc
     1681 ggtaccctcg acgcatcgac ctcagatcac ctctaccgca tggttcagga ggcgatcacc
     1741 aacgtggcgc ggcatgccca tgcgcggcgc gccgagatca gcacacgcct cgaagacggt
     1801 gccctgatca tcgaagtgag cgacgatggc atcggcattc cggccagcgt cagcaacggc
     1861 tttggacttc gcacgattcg ctaccgggcg caggctatcg gagccgagtt cgcgatcaat
     1921 gccgcccggc cgcgcg
//
LOCUS       DASWZT010000494         3844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_52322, whole genome shotgun sequence.
ACCESSION   DASWZT010000494 DASWZT010000000
VERSION     DASWZT010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52322"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VGE92_14175"
     CDS             complement(<1..732)
                     /locus_tag="VGE92_14175"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY1315023.1"
                     /translation="MNAAVALARSEIVTLKPYAHAAWLPSLTRLHANEVPWRPAGDTT
                     AGGLNRYPEPQPHALVERLAALYGVPAANLLATHGSDEAIDLLSRIYLRPGIDAILQP
                     TPTFGMYQVAARIQGAGVVEIPLDRERGWALDPQRLLAAWHPNIKLVYLCSPNNPTSN
                     LLATAAIEEVCQALDGKAIVVIDEAYLEWSHAASATRWLNRFSTLAILRTLSKAHALA
                     GARIGALLANAALIELARRIAPPYSL"
     gene            complement(729..2039)
                     /gene="hisD"
                     /locus_tag="VGE92_14180"
     CDS             complement(729..2039)
                     /gene="hisD"
                     /locus_tag="VGE92_14180"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY1315024.1"
                     /translation="MRILDWNSLSAAQRRDALARPAQRDAAAIHAAARASIEAVRRDG
                     DAAVLALTERFDHVRLDSMRVTPQEFTAAERTLSAEQTTALARAIANVRRFHAEQGAP
                     ALRVETAPGVVCERRSVALRAVGLYVPAGSAPLPSTAVMLAVPATIAGCRLRIMCTPP
                     NRQGAADPGVLTAARMAGIDLVFKVGGAQAIAAMAYGTASIPKCDKIFGPGNAYVTAA
                     KMLVAQDAAGAAADLPAGPTEVMVVADDSARADFVAADLLAQAEHGPDAQSLLVTDAP
                     ALAEEVARQVARQAPLLSRAAILSRSIEHMRLCNVDCLDTAFEIANEYAPEHLLLELR
                     NPRAWLDRVSAAGAVFLGHWSPESMGDYCSGPNHTLPTYGFARAYSGLSLEDFQKRIT
                     VQELTPAGLADLGSTAQVLANLEGLDAHAAAVAIRLAALAGAPA"
     gene            complement(2051..2968)
                     /gene="hisG"
                     /locus_tag="VGE92_14185"
     CDS             complement(2051..2968)
                     /gene="hisG"
                     /locus_tag="VGE92_14185"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEY1315025.1"
                     /translation="MANSLPAPDPMRLKIAVQKSGRLTDSSLELLSRCGLKYSRGKDQ
                     LMCYGENMPLDVLFVRDDDIPDLVQEDVCDLGLVGLNVVQEKRLAFAARSIAPLFETV
                     QLLDFGRCKLCIAVPDGFDYCDPRSLQGKRIATTYPHILGRFLADHGIAAEVVVLSGS
                     VEIAPRLGRADLICDLVSTGSTLAANHLRQAHIVLESQAVLIRTPVPIAPQKQEWARR
                     LLMRIDGVEQVKGSKYIMLHAPRSALPAIAKLLPGSEAPTVIPLEGRGDRVAVHAVCR
                     ENVFWETLEELKGAGATSVLVLPVEKMLA"
     gene            complement(2961..3272)
                     /locus_tag="VGE92_14190"
     CDS             complement(2961..3272)
                     /locus_tag="VGE92_14190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HEY1315026.1"
                     /translation="MKTNRPVTLRQEREHERALCTAILALHSLEECRSFFRDLCTPAE
                     LQAMADRWAVVELLERGLPYREIHKQTGVSVTTIGRVARYLISGNGGYRAVTERLKLD
                     G"
     regulatory      3396..3526
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            3594..>3844
                     /locus_tag="VGE92_14195"
     CDS             3594..>3844
                     /locus_tag="VGE92_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315027.1"
                     /translation="MAEKPSNPIDQTLKLAAPFTLHYGDVLEDAKIAYRLIGPENAPV
                     IVVLGGISAHRIVAGAAGEGWWPEIVGPGLGVDTRHFRVL"
BASE COUNT          694 a         1258 c         1247 g          645 t
ORIGIN      
        1 cagcgagtag ggcggagcga tgcgtcgcgc cagctcgatg agcgccgcat tcgccagcag
       61 cgcaccgatt ctggcgcccg ccaatgcatg cgccttggat agagtgcgca aaatcgccag
      121 ggtcgagaat cggttcagcc agcgcgttgc actcgcggcg tgcgaccact cgaggtacgc
      181 ctcgtcgatc acgacgatgg ctttgccgtc gagcgcctga caaacctctt cgatggcggc
      241 cgtcgccagc aggttcgagg tcggattgtt cggcgagcac agatacacga gcttgatatt
      301 gggatgccag gcggccaaca gccgttgcgg gtcgagcgcc cagccgcgct cgcggtcgag
      361 cgggatttcg accaccccgg cgccctgaat gcgcgcggca acttgataca tgccgaacgt
      421 cggcgtgggt tgcaaaatgg cgtcgatgcc cggccgcaga tagatgcgtg acaacaggtc
      481 gatcgcttca tcgctgccgt gcgtcgcgag cagattcgcg gcgggaacgc cgtacagcgc
      541 ggccagccgt tcgaccaagg catgcggctg cggctccgga tagcgattca gaccgcccgc
      601 cgtcgtgtct ccggccggcc gccaaggcac ttcgtttgcg tgcagacgcg tcagcgacgg
      661 cagccatgcg gcgtgcgcat agggtttgag agtaacgatt tcgctgcgcg ccagcgcgac
      721 cgcggcgttc atgccggggc tccggcaagc gcggcgaggc gaatcgcgac tgccgccgca
      781 tgcgcgtcca ggccctcgag attggccagc acttgcgcgg tggatcctaa gtccgcgagc
      841 ccggcgggcg tcaactcctg gaccgtgata cgtttttgaa aatcctccag cgacagaccg
      901 ctgtaggctc tggcaaatcc gtaggtcggc aacgtgtggt tgggcccgct gcagtagtcg
      961 cccatggatt ccggcgacca atgccccaag aacaccgcgc cggcggcgct gactcgatcc
     1021 agccaggcgc gcgggttgcg cagctcgagc aacaagtgct cgggagcgta ctcgttggca
     1081 atctcgaacg cagtgtcgag gcagtcgacg ttgcacagcc gcatgtgctc gatggagcgc
     1141 gacaggatcg ccgcgcgcga cagcagcggc gcctggcgcg ccacttgacg cgccacctcc
     1201 tcggccaatg ccggagcatc cgtgaccagc agcgattgcg catcgggacc gtgctccgcc
     1261 tgcgccagca gatcggcagc cacgaagtcg gcgcgggcgc tgtcatccgc aaccaccatc
     1321 acttcggtcg ggccggccgg aaggtccgcc gcggcgcccg cagcgtcctg cgccaccagc
     1381 atttttgctg ccgtgacgta cgcattgccg gggccgaata tcttgtcgca cttcggaatg
     1441 ctggcggtgc cgtaagccat tgcggcgatc gcctgtgcgc cgcccacttt gaagaccaga
     1501 tcgatgcctg ccatgcgcgc cgccgtcagc acgccgggat ccgcggcacc ctggcggttc
     1561 ggcggcgtgc acatgatgcg caggcggcag ccggcgatgg ttgcgggaac cgccagcatg
     1621 acggcggtcg aaggcagcgg agccgagcct gcggggacat acagaccgac cgcgcgcagc
     1681 gcaacgctgc gccgctcgca aacgactccg ggcgccgttt cgacgcgcaa cgccggcgcg
     1741 ccttgctcgg catgaaagcg ccgcacgttg gcaatcgccc gggcgagcgc cgtcgtctgc
     1801 tcagcgctca aggtgcgctc cgcggccgtg aattcctgcg gcgtcacgcg catcgagtcc
     1861 aagcgcacgt gatcgaatct ctcggtgagc gccaatacgg ccgcgtcgcc gtcgcggcgc
     1921 accgcttcga tgctagcgcg cgccgccgcg tgaattgccg ccgcatcgcg ttgcgccggg
     1981 cgcgccaatg cgtcgcggcg ttgtgccgcg gatagggaat tccaatcgag aatgcgcatg
     2041 ctgccgtgac ctacgcgagc atcttctcga ccggaagcac cagcaccgag gttgcacccg
     2101 cgcccttgag ttcttcgagc gtttcccaaa acacgttctc gcggcagacg gcgtgcacgg
     2161 ccacgcggtc accgcgccct tcgagcggaa tcaccgtcgg tgcctcgctg cccggcagaa
     2221 gcttggcgat ggccggcagc gcgctgcgcg gtgcatgcag catgatgtac ttgctgcctt
     2281 tgacttgctc gacgccgtcg atgcgcatca acaggcgccg cgcccactcc tgcttttgcg
     2341 gcgcgatcgg caccggagtg cggatcagca ccgcctggct ttcgagcacg atgtgtgcct
     2401 ggcgcagatg gttggccgcg agcgtcgagc cggtcgacac cagatcgcaa atgaggtcgg
     2461 cgcggcccag ccgcggtgcg atctccacgg atccggaaag aaccaccacc tccgcggcga
     2521 tgccgtggtc cgcgaggaaa cgccccagaa tgtgcggata ggtcgtcgcg atgcgcttgc
     2581 cctgcagcga tcgcggatcg cagtaatcga atccgtcggg cacggcgata cacagcttgc
     2641 agcggccgaa atcgaggagc tgcaccgttt cgaacaatgg cgcgatgctg cgcgcagcga
     2701 acgcgagccg cttctcctgc acgacattga gcccgaccag acccaagtcg cacacatctt
     2761 cctgcaccag gtcgggaatg tcgtcgtcgc gcacgaacag cacgtcgagc ggcatgttct
     2821 cgccgtagca catgagctgg tccttgccgc ggctgtactt cagcccgcag cgcgacaaca
     2881 gctcgagcga actgtcggtc agccgtccgc tcttctgcac tgcgatcttg aggcgcatcg
     2941 gatcgggcgc gggcagtgag ttagccatcg agtttcaagc gctctgtcac ggcgcgatag
     3001 ccgccgttgc ccgagatcaa gtaacgggcg acgcgcccga tggtggtgac gctgaccccg
     3061 gtttgcttgt gaatctcgcg gtagggtagg ccacgctcga gaagctcgac caccgcccaa
     3121 cggtcggcca tggcctgcag ctcggccgga gtgcacagat cgcggaaaaa actgcggcac
     3181 tcctcgaggc tgtgcagcgc caatatcgcc gtgcacagcg cgcgctcgtg ctcacgttct
     3241 tgccgcaatg tgaccggacg gttggttttc atgaattcgc aatgctcgcc aatgtattaa
     3301 cgcactaata cattaataca ttgatatggc gggcgcaagc aaaaacgccg caaaatgacc
     3361 ttgacgcaaa catgggcaat ccttaaaatt cccaaagctt atctagaccg gctgagggaa
     3421 aggcccttag aagccggggc aaccacccct tggttcccat ggaatcgagc ggaacggtgc
     3481 caactcctgc ggaatcggac taacacccgg atccggtaga taggcggtcg gtttcgcgac
     3541 gagcgcgggc gctacagggg ttatgtaaac ctctgtatgg agcgaagtgc aacgtggcgg
     3601 aaaaaccctc aaacccgatc gaccaaaccc tcaagctggc tgcgccattc acattgcatt
     3661 acggcgatgt cctggaggac gccaagatcg cctatcggct gatcggcccg gagaatgcgc
     3721 cggtgatcgt ggtgctcggc ggcatttccg cacatcgcat agtggccggg gccgcgggcg
     3781 agggctggtg gccggagatc gtcggtcccg gtctcggcgt cgatacacgg cacttccgcg
     3841 tcct
//
LOCUS       DASWZT010000495         4805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_57229, whole genome shotgun sequence.
ACCESSION   DASWZT010000495 DASWZT010000000
VERSION     DASWZT010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57229"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2396)
                     /gene="mprF"
                     /locus_tag="VGE92_14200"
     CDS             complement(<1..2396)
                     /gene="mprF"
                     /locus_tag="VGE92_14200"
                     /inference="COORDINATES: protein motif:HMM:NF033480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEY1315028.1"
                     /translation="MAHPVTSRKVPAWTTLLGIALFGLALYWLHHLLGQYRWQDILGH
                     VHAIPPAKVLRAGLFTLAGYGCLTLYDALAVHFAGARVPYPRIALISFMGYAIGHNVG
                     LNTLSGGAIRYRAYSALGLGAKQIATIIAFGTVTFLLGAGFLLGLSLLSQAGMSGSVL
                     HVHASLAMLAGGVLLAAVAAYLWLVCNRHKPLKLGPVFIPVPKPRIAFAQLAVACADL
                     LCAASVLYVLLPEQATLSFAAFAGVYLIAIAAGVISNVPGGIGVFETVLLLLLRSVPQ
                     DRLLGALVAYRAIYYFLPFGIALALLGAHELWVHRGPAVRLARLGRTFLIAVTPQAIA
                     IAVFVAGAVLLFSGATPALHSRLDLLRTFVPLPILELSHLLGSAVGVGLLVIAHGLYR
                     RLDAAWWLTVWLLCAGILLSLLKGFDYEEAIILGVVVIVLVSARARFKRRASLIEQHY
                     SGPWIIALCIVLATAAWLVVFAYRHVPYGNQLWWEFAFHASAPRSLRASLLAAIIAAA
                     YGLWRLLRPATPPMSAPSEADMERVAELAAAAGDTTANLALLGDKNLLFNAERTAFIM
                     YQVSGHSWVAMGDPVGPPELCESLAWEFLENCDVMAVSPVFYQVKPENLPLYIDLGLN
                     LSKLGEEARVPLDKFSLEGPARADLRQAHRRANRDGAQFQLIPRAEVAAILPELRAVS
                     DAWLAEKNTAEKRFSLGFFDERYLRHFDCGVVRRGGAVVAFANIWRGGSTELSIDLMR
                     YGGDAPKGVIDYLLIECMLWGKSQGYHWFNLGMAPLSGLEEHALAPTWHKLGRMVLRY
                     G"
     gene            2488..2886
                     /locus_tag="VGE92_14205"
     CDS             2488..2886
                     /locus_tag="VGE92_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315029.1"
                     /translation="MSSATDGRQAFMDSTQIYREETFTDRKVGTIRRMTPVAADGSED
                     SARPVLFVGQAQVMTPMGALPISFELDATSLDAAIEKFGPAAEQAVQQTMRELQEMRR
                     EQASSLVIPDAGGAALPNPSDLLGRGRPRR"
     gene            complement(2883..4664)
                     /locus_tag="VGE92_14210"
     CDS             complement(2883..4664)
                     /locus_tag="VGE92_14210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1315030.1"
                     /translation="MPIVRLDKVSLSFGLKPLLDAVDLHIVRGERVCLLGRNGEGKSS
                     LLQLITQQIVPDSGAVWVRPGARVASLAQEVSPASEASVREVVMSGFEPGGAEHADWQ
                     SELQADQVISRLQLDRDASMASLSGGWRRRVMLGRALVAHPNLLLLDEPTNHLDIDAI
                     TWLEEMMLDFDGALLFISHDRAFVRRLATRIIELDRGRLRGWPGSYDEYVVQKRAALE
                     VEARHAALFDKKLAQEEAWIRQGVEARRTRNEGRVRALKQLRLQRSERRERIGQVEIR
                     VQEATTSGKLVFEAAHLTQSFGAATVIADFSTRIMRGDRVGIVGPNGCGKTTLIKLLV
                     GELEPTSGSVRRGTGLLPAYFDQQREQLDLSASIMDNVTGGSGDTVTIDGQPRHVSGY
                     LRDFLFPPERLLAPVSMLSGGERNRLLLARLFARPSNLLVMDEPTNDLDAETLELLEE
                     MVATYAGTLLLVSHDRAFLDNVVTSTLVFEGAGQINEYVGGYSDWLRQRRSASVPQAR
                     ASTTPSAAAPAAKRARRLSFKEQGELAAMPDRIQQLETEQLALQAALADPQLFKDPAR
                     GTATVKRLEALAAQLEQAYSRWHALES"
BASE COUNT          968 a         1542 c         1514 g          781 t
ORIGIN      
        1 ccgtagcgca gcaccatgcg tccgagcttg tgccaggtgg gcgcgagcgc gtgttcttcc
       61 aatcccgaca gcggtgccat tcccagattg aaccagtgat aaccctgcga cttgccccac
      121 agcatgcact cgatcaatag atagtcgatg acgcccttcg gcgcgtcgcc gccgtagcgc
      181 atgaggtcta tggatagttc agtgctgccg ccgcgccata tgttggcgaa tgcgaccacc
      241 gcgccgccgc gccgaaccac tccgcagtcg aagtgccgca gatagcgctc atcgaaaaaa
      301 cccaaggaaa agcgcttttc ggcggtgttc ttttccgcca accaagcgtc ggacaccgca
      361 cgcagttccg gcaatattgc cgcaacctcc gcgcgcggaa tgagttggaa ctgcgcgccg
      421 tcgcggttgg cgcgccgatg cgcctggcgc aagtcggcgc gcgcaggccc ctccagcgag
      481 aacttatcga gcggcacgcg cgcttcctcg ccgagcttgc tcaagttcaa tccgagatcg
      541 atgtacagcg gtagattctc gggtttgact tgatagaaca cgggcgatac cgccataacg
      601 tcgcaattct ccagaaattc ccatgccaag gattcgcaaa gctcgggcgg tccgaccgga
      661 tcgcccatcg ccacccaact gtggccggaa acctggtaca tgatgaatgc ggtacgctcg
      721 gcgttgaaca gcaagttctt gtcgccgagc aatgccagat tggcggtcgt atcgccggct
      781 gcggcggcca gttcggcgac gcgttccata tccgcctcgc tgggcgccga catcggcggc
      841 gtcgccggcc gcagcagccg ccacagccca taggcggccg cgatgatcgc ggccagcagc
      901 gaagcgcgca agctgcgcgg cgccgatgcg tggaacgcga attcccacca cagttggttg
      961 ccgtacggca catggcggta ggcgaatacc accagccatg cggcggtcgc gagaacaatg
     1021 cacagcgcga tgatccatgg gccggaatag tgctgttcga tcagcgaagc acgccgcttg
     1081 aatcgcgcac gtgccgacac cagcacgatg acgacgacgc caagaatgat cgcctcttcg
     1141 tagtcgaagc ccttcagcag cgacagtaga attccggcgc acagcagcca caccgtcaac
     1201 caccaggccg cgtcgaggcg ccggtaaagg ccgtgcgcaa tcaccagcag gccgacaccc
     1261 acggcgctgc cgagcaaatg cgagagttcg aggatcggca acggcacgaa ggtgcgcagc
     1321 aagtcgaggc ggctgtgcag cgcgggcgtg gcgccggaga acagcagcac ggcgcccgcc
     1381 acgaacaccg cgatagcgat ggcctgcggc gtgacggcga tcaaaaacgt ccggccgagc
     1441 cgcgccaggc gcaccgccgg gccgcgatgc acccacagct cgtgcgcgcc gagcagtgcc
     1501 agggcgatgc cgaaaggcaa gaaatagtaa atggcgcgat acgcaaccag agctcccaac
     1561 aggcggtcct gcggcacgga tcgcagcagc agcagcaaca cggtctcgaa gacgccgatg
     1621 ccgccgggaa cgttgctgat cacgccggcc gcaatagcga tcaggtaaac gccggcgaat
     1681 gccgcgaagc tgagcgttgc ctgctccggc aacaacacgt aaaggacgct tgccgcgcac
     1741 aataggtccg cgcacgccac cgccagctgc gcaaatgcga tgcgcggttt ggggaccgga
     1801 atgaacacgg ggccgagctt caagggtttg tgacggttac ataccagcca gaggtaggcc
     1861 gcgacggcgg caagcagcac gcctcccgcg agcatggcga gcgaggcatg tacatgcagc
     1921 accgaccccg acatgccggc ctgcgacaga agcgacagtc ccaggagaaa cccggcgccc
     1981 aataagaaag tgacagtgcc gaacgcaatg atggttgcga tttgtttggc accgaggcca
     2041 agcgccgaat aggcgcggta gcgaattgcc ccgccgctca aggtgttgag gccgacattg
     2101 tggccgatgg cgtaacccat gaaggagatc agggcaatgc gcggatacgg tacgcgtgca
     2161 ccggcaaagt gcacggcaag cgcatcgtag agggtcaagc aaccgtagcc cgcaagcgta
     2221 aacagcccgg cgcgcagcac tttcgcgggc ggtatggcgt ggacgtggcc taagatatcc
     2281 tgccagcgat actggccgag cagatgatgc agccagtaca acgccagccc gaatagggca
     2341 atgcccaaca gagtggtcca cgctgggacc ttgcgagatg tgacggggtg tgccaagcga
     2401 gatatcgtag cagggcctcc cgtacggcgc tgcaaactgc tagggttgag tcttgaagct
     2461 gaaacgatca caaggggtag cccacgcatg agttccgcca ccgacggccg ccaggcattc
     2521 atggactcga cgcagattta tcgcgaagaa acctttaccg accgcaaagt gggcacgatc
     2581 cgccgcatga cgccggtcgc cgccgacggc tccgaggatt ccgcgcggcc ggttctgttt
     2641 gtaggacaag cgcaggtcat gacgccgatg ggcgcgctgc cgatcagctt cgaactcgac
     2701 gcgacctcgt tggacgcggc gatcgaaaaa tttggccctg ccgcggaaca agccgtgcag
     2761 cagaccatgc gcgaactgca ggagatgcgc cgcgaacagg catcatccct ggtcattccc
     2821 gatgcaggtg gcgccgctct gccgaatccg agcgatctgt tgggccgcgg acgtccgcgg
     2881 cgctaagatt ccagggcgtg ccaccgggag tacgcttgct cgagctgcgc ggcgagagcc
     2941 tctaagcgct tgacagtggc cgtgccccgc gccgggtcct tgaataactg cggatcggcg
     3001 agcgcggctt gcaacgccag ctgttcggtt tcgagttgct gaatcctgtc gggcatggca
     3061 gcgagctctc cctgctcctt gaaggagagc cgtcgggctc tcttcgcagc cggcgccgcc
     3121 gctgacggcg tggtggaagc ccgcgcctgc ggcacgctgg cggatctgcg ctggcgcagc
     3181 caatcgctat agcctcccac atattcattg atctgccctg cgccttcgaa caccagcgtg
     3241 ctggtgacga cattgtcgag aaacgctcgg tcatggctta ccaacagcag cgtcccggcg
     3301 taagtcgcca ccatctcttc gagcagctcg agagtttctg cgtccagatc attggtgggt
     3361 tcgtccatca cgagcaggtt gctcggccgg gcgaacagcc gcgcgagcag cagtcgattg
     3421 cgttcgccgc cggataacat gctgaccggc gccagcagcc gctccggtgg aaacaggaaa
     3481 tcgcgcaaat agccggagac gtggcgcggc tgtccgtcga tcgtgaccgt gtcgccgctg
     3541 ccgcccgtga cgttgtccat gatggaggcc gagaggtcca gttgctcgcg ctgctggtcg
     3601 aaatacgcgg gtagcaggcc ggtgccgcgg cgcaccgatc cggaggtcgg ttcgagctcg
     3661 ccgaccaaca gctttatcag cgtcgttttg ccgcagccat tgggacccac gataccgacc
     3721 cgatcgccgc gcatgatgcg cgtcgagaaa tccgcgatca cagtggccgc gccgaaactc
     3781 tgcgtcaggt gcgctgcctc gaacaccagc ttgcccgacg tagtcgcctc ctgcacgcga
     3841 atttccactt ggccgatgcg ctcgcgccgt tcgctgcgct gtagacgcag ctgtttgagc
     3901 gcgcgtactc tgccctcgtt gcgcgtgcgc cgtgcctcga cgccttgtcg gatccacgcc
     3961 tcctcttgcg caagcttctt gtcgaagaga gccgcgtgtc tcgcctccac ttccagggcg
     4021 gcgcgtttct gcacgacgta ttcatcatag ctgcccggcc agccgcgcag cctgccgcga
     4081 tcgagttcaa tgatgcgagt cgccaggcgg cgtacgaacg cgcggtcatg gctgatgaac
     4141 aacagcgcgc cgtcgaagtc gagcatcatt tcttcgagcc aggtaatggc gtcgatgtcg
     4201 agatgattgg tcggctcatc caacaacaac agattcggat gggcgaccaa ggcgcggccc
     4261 agcatcaccc gccgccgcca accaccggac aacgaagcca tcgatgcgtc gcgatcgagt
     4321 tgcaagcgcg agatgacctg gtcggcttgc aattcggact gccaatcggc gtgttcggca
     4381 ccgcccggct cgaatccgct catgacgacc tcgcgcacgc ttgcctcgct cgcgggcgat
     4441 acctcctggg ccaaactcgc gacgcgcgcg cccgggcgca cccataccgc gccgctgtcc
     4501 gggacaattt gctgcgtgat cagctgcaac agcgaggatt ttccctcgcc gttacggccc
     4561 agcaggcaaa cgcgctcgcc cctgacgatg tgcaggtcga cggcgtcaag caagggtttg
     4621 aggccgaaac ttaaggagac tttatcgagc cgaacgatgg gcatgaggcc gcgtatagta
     4681 tgcggctcaa gctcaatatc ccagccacaa tgagaggtag atgccgcgct gcggcggctt
     4741 gccggcaata cggcccagat cggtccaggc gctggtgagc gagagattct tgatcggggc
     4801 ccaag
//
LOCUS       DASWZT010000496         4782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_8747, whole genome shotgun sequence.
ACCESSION   DASWZT010000496 DASWZT010000000
VERSION     DASWZT010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8747"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..2018
                     /gene="recQ"
                     /locus_tag="VGE92_14215"
     CDS             192..2018
                     /gene="recQ"
                     /locus_tag="VGE92_14215"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEY1315031.1"
                     /translation="MELTAALKQIFGFEGFRTGQEAVVRDAMAGRDVLALMPTGGGKS
                     LCFQLPAMLQSGVTLVVSPLIALMQDQVRLLRDNDIAASFINSSLDAADVSRRIAALL
                     RGDFKLFYLAPERLLMREFLDGPLRLLQQGPGINAFVIDEAHCVSEWGHDFRREYRQL
                     SALRRRHPLVPILAFTATATPRVRSDIVTQLALRDPAVHVASFNRPNLFYQVRAKGKR
                     TYAELLAQARAGGAGIVYCLSRRRVDELTEQLHADGIRARPYHAGLDSVVRRDNQDAF
                     IRDDIDVIVATIAFGMGIDKPDVRWVVHYDLPKSLEGYYQESGRAGRDGDAARCTLYF
                     GAADIRTAEFLIQQKIDPNNGEPLVDEQRIARQQLRQVQNYAESTGCRRAIQLRYLGE
                     SFATPCAACDNCCEPRPQQDCSLEARQFLSCVARLAQRGQRFGAAQIIDILRGSRSER
                     VLARGHDTLSVYGIGKHRSLDDWRSVGRALLHQGLIEETQDGFPVLSLNAQSGKVLRE
                     ELAVHIAAPLKRPRNSADALLPTTPQSDELFEHLRALRKRLADRHGLPPYVVFHDATL
                     RKMAERRPTTLSQFAELPGVGQAKLARYGESFIAAIGQFSSL"
     gene            complement(2000..3310)
                     /locus_tag="VGE92_14220"
     CDS             complement(2000..3310)
                     /locus_tag="VGE92_14220"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY1315032.1"
                     /translation="MKSNMTGMRLYLPLFTMCLAVQAAGAAPAGAEPAPLAIRAGKLI
                     DVETGRVLSNEIILVRGTKIEAVGTNLTLPEGARVIDLSKMTVLPGLIDCHTHLADLA
                     DAEPLNVLQRSAVEAAYAAIPNASVTLLAGFTTVRDVGVYRAFNDVAMRDAIARGIII
                     GPRMYVAGAYITISQGAGAMTGLAPDIQLPLDLRYGEANSPWEVRQKIRELAHRGADH
                     IKVLSTGAVLTHGSNPKSIEFTAEELRAAVEEAANFGMRVAAHAHAAEGIKNAIRAGV
                     ASVEHATLIDDEGIALAKQHGTYLDMDIYDEECIQSAANTPADFLQHDQELAAAQRRN
                     FTKAVRAGVKMTFGTDAGVCPHGINARQFAFMVKYGMTPMQAIQAATVNAADLIGHSE
                     LFGSITAGKSADIVAVNGDPLADIRLLEHVQFVMKEGKVYKDEN"
     gene            complement(3323..4342)
                     /locus_tag="VGE92_14225"
     CDS             complement(3323..4342)
                     /locus_tag="VGE92_14225"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEY1315033.1"
                     /translation="MKPGDLGFSMPAEWAAHSGCWMGWPQREALWHGHMEAARDDYAK
                     VAQAIAQFEPLTMLAAPEHAADAQARCGAKIRVLAVPLDDSWLRDSGPTFVLNAAGER
                     AAAVFTFNAWGAKFHPFDQDAALGAKVAELASARVFKSDLVIEGGGFLSDGEGTLITA
                     ESCVLNANRNPGWTKAQADAELRSMLGVEKIIWLPGDISDDSTDGHVDGYVAFTRPAA
                     VLCESVADPDDPRFAIMAENRKVLETETDARGRRFELLPIPEAPRTAVPAGEDIYCRS
                     YVNFYLANGAVIAPLYGIAEDAAARDALQRAYPKLRVVMLELRDLFRGGGGIHCITQQ
                     EPRGR"
     gene            complement(4339..>4782)
                     /locus_tag="VGE92_14230"
     CDS             complement(4339..>4782)
                     /locus_tag="VGE92_14230"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY1315034.1"
                     /translation="LLGDARLTLAPAGIGLALLSGLCWALYCLFRLKWRGAAARVLAR
                     GCAVSTFLCAVLHLLLEPTIVPSLGAFAAAAAIGIVPLALGNFVWDQGFRRGDSRLLA
                     VLAYATPVCSALLLAALGLAAMTWSLLAGAALIACAGLLSRENAS"
BASE COUNT          871 a         1554 c         1482 g          875 t
ORIGIN      
        1 aggtgtaact tcacggcgcc tcccgctaaa tgaccgttct gcagccatga tagccgatcc
       61 gcgagcgcgg ccgcggcagc cggatcaacc gtctccgaac accgactaat cggcgtcaat
      121 cgcgcacatt cccggcgccc ctcatttccc atggccctat acaatggcgc tttaacttaa
      181 ccggggtgct ggtggaactg actgcggcgt tgaagcaaat cttcggcttc gaagggtttc
      241 gtaccgggca ggaagcagtg gtacgcgacg cgatggcggg ccgcgacgtg ttggccctga
      301 tgcccactgg cggcggtaaa tccctgtgtt ttcagctccc cgcgatgttg caatcgggcg
      361 tcacgctggt ggtatcgccg ctcatcgccc tgatgcagga tcaggtgcgc ctgctgcggg
      421 acaacgatat tgccgcgagc ttcatcaact cgagcctgga tgcggccgat gtgtcgcggc
      481 gcattgccgc gctgctgcgc ggcgatttca agctgttcta tctggcgccg gaacgactct
      541 tgatgcgcga atttctggac ggcccgctgc gcctcctgca gcaaggtccc ggcatcaacg
      601 ccttcgtcat cgacgaagcc cactgcgtct cggagtgggg ccacgatttt cgccgcgagt
      661 atcggcaact gtccgcgttg cgccggcgcc atccgctcgt tccgatactg gcgtttacgg
      721 ccacggcgac cccgcgcgtg cgcagcgaca tcgtgacgca gctggcgctg cgcgatcctg
      781 ccgtgcatgt ggcgagcttc aatcgtccca atcttttcta ccaagtgcgc gccaagggca
      841 agcgaaccta cgccgagctg ctggcacagg cccgagccgg cggcgccggc atcgtttatt
      901 gtctctcgcg ccggcgtgtc gacgaattga cggaacagct gcatgctgat ggcatccgcg
      961 cacgtcccta tcacgccggg ctcgactccg tggtccggcg cgacaatcaa gacgcgttca
     1021 tccgcgacga tatagatgtc atcgtggcaa ccatcgcgtt cggcatgggg atcgacaaac
     1081 ccgatgtgcg ctgggtcgtt cactatgatc tgccgaaatc cctggagggt tattaccaag
     1141 agtccggccg agccgggcgc gacggcgatg ctgcgcgctg cacactgtat ttcggcgctg
     1201 ccgacatacg caccgcggaa tttctcattc agcagaaaat cgatcccaac aacggcgagc
     1261 cgctggtcga tgagcagcgc atcgcgcgtc aacaattgcg ccaggtgcag aactacgccg
     1321 aatcgaccgg gtgccgccgt gcgatccaat tgcgatactt gggcgagagc ttcgccacgc
     1381 cttgcgcggc ttgcgacaac tgctgcgaac cacgcccgca gcaagactgc agcttggagg
     1441 cccgccagtt cttgtcctgc gtcgcgcgcc tcgcgcagcg cggccagcgc ttcggcgcgg
     1501 cgcaaatcat cgacattctg cgcggttcgc gcagcgaacg cgtgctcgcg cggggacacg
     1561 acacgctgag cgtttacggc atcggcaagc atcgatcgct cgacgattgg cgcagcgtag
     1621 ggcgtgccct gctccaccag ggactcatcg aggagactca ggacggcttt ccggtgctgt
     1681 cgctcaatgc gcagagcggc aaagtgctgc gcgaagaact cgccgtgcat atcgcggccc
     1741 cgctcaagcg gccgcgcaac tccgcagacg ccctgctccc aacaactccg caaagcgacg
     1801 agttattcga gcatctgcgc gcactgcgca agcgcttggc ggaccggcac ggcctccctc
     1861 cctacgtcgt gttccacgac gcgacgctgc gcaagatggc cgagcgtcgg ccgaccaccc
     1921 tgagtcagtt tgcagaactt cccggcgtcg ggcaggccaa gttggcgcgt tacggggagt
     1981 ccttcatcgc cgcgatcggt cagttttcgt ccttatagac cttgccttcc ttcatcacga
     2041 attgcacgtg ttcgaggagg cggatatccg cgagcggatc gccgttcacg gcgacgatat
     2101 ccgccgactt gccggccgtt atggaaccga acagttccga gtgaccgatg aggtcggcgg
     2161 cattgacggt cgccgcttga atcgcctgca tgggagtcat gccgtatttc accatgaagg
     2221 cgaattgccg ggcgttgatt ccatgcgggc aaacgcctgc atcggtgccg aacgtcattt
     2281 tgacgccggc gcgaaccgct ttggtgaagt tgcggcgttg tgccgcggcg agctcttggt
     2341 cgtgctgcag gaagtcggcc ggcgtattgg cagccgactg aatgcactct tcatcgtaga
     2401 tatccatatc gaggtaggtg ccatgctgct tggcgagcgc gatgccttca tcatcgatca
     2461 gcgtagcgtg ttcgaccgac gcgacgccgg cgcgaatcgc attcttgatc ccctcggcgg
     2521 catgcgcgtg cgccgcaacg cgcatgccga aattggccgc ttcctcgacg gcggcacgca
     2581 gctcttccgc agtgaattcg atggatttcg gattgctgcc gtgggtgagc accgctcccg
     2641 tggacagcac cttgatgtga tcggcgccac ggtgcgccag ttcgcgaatt ttctggcgca
     2701 cttcccacgg actattcgct tcaccgtagc gcaaatccag cggcagctga atatcgggcg
     2761 cgaggccggt catcgcgccc gccccttggc tgatcgtgat gtaggcgccc gcgacataca
     2821 tgcgcgggcc aatgatgatg ccgcgcgcga tggcatcgcg catcgccaca tcgttgaagg
     2881 cgcggtacac gcccacatcg cgcacggtgg tgaatcccgc gagcagcgtc acgctggcat
     2941 tgggaattgc agcgtaggcg gcttccaccg cgcttctttg cagtacgttg agaggttccg
     3001 cgtcggcgag atctgcgaga tgcgtatggc aatcgatcaa gccgggaagc accgtcatct
     3061 tcgatagatc gatgactcga gcgccttcgg gcagggttag attcgtgccg accgcctcga
     3121 tcttggtacc gcgaaccaga atgatctcgt tcgacagcac gcgacccgtc tcgacgtcga
     3181 tcaacttacc ggcacggatg gccagcggcg cgggctctgc gcccgcgggc gctgcccccg
     3241 ccgcttgcac ggcaaggcac atcgtaaaca gcggcaggta aagccgcata ccggtcatat
     3301 tgcttttcac gagttgtcct ctctagcggc cgcgcggctc ttgttgtgtg atgcagtgaa
     3361 taccgccgcc gccgcgaaat aaatcccgca actcgagcat caccacgcgc agcttgggat
     3421 acgcgcgctg cagcgcgtca cgggccgccg catcctcggc gatcccgtag agcggtgcga
     3481 tgacggcgcc gttcgccaga tagaaattca catacgaacg gcaatagatg tcctcgcccg
     3541 cgggcaccgc cgttcgcggt gcttccggta tgggcagcag ctcgaagcgc cggccgcgcg
     3601 cgtcagtctc ggtctccagg actttgcgat tttccgccat gatggcgaat cgcggatcat
     3661 ccggatcggc caccgactcg cacagtaccg cggccggccg agtgaaagcc acgtatccat
     3721 cgacgtggcc gtcggtcgag tcatcgctga tgtctcccgg cagccagatt attttctcga
     3781 cgccgagcat gctgcgaagc tcggcgtcgg cctgcgcctt ggtccatccg ggattgcggt
     3841 tggcgttgag cacgcacgat tctgcggtga tgagagttcc ctcgccatcg gatagaaagc
     3901 cgccaccctc gatgacgaga tccgatttga aaacgcgcgc tgacgcgagc tccgcgacct
     3961 ttgcaccgag cgccgcgtcc tgatcgaagg ggtggaattt ggcgccccag gcgttgaacg
     4021 taaataccgc ggcggcgcgt tcgccggcag cgttcaaaac gaatgtcggt ccggaatcgc
     4081 gcagccatga gtcgtccaag gggacggcca gcacgcggat cttggctccg cagcgtgcct
     4141 gtgcatcggc cgcgtgttcg ggagcggcca acatggtgag cggctcgaat tgggcgatcg
     4201 cctgcgcgac cttggcgtag tcgtctcgcg cagcctccat gtggccgtgc caaagcgctt
     4261 cgcgttgcgg ccagcccatc cagcatccgg aatgcgcagc ccattcggcc ggcatcgaga
     4321 aacccaagtc gccgggcttc aagacgcatt ctcgcgcgac agcaagccgg cgcatgcgat
     4381 gagcgcggcg cccgccaaca gactccatgt catcgccgcc aacccgagtg ctgccagcaa
     4441 caaggcactg cacacggggg tcgcataggc gagcaccgcg agcagtcggc tatcgccgcg
     4501 gcgaaagcct tgatcccaga cgaaattacc gagcgccagc ggcacgatac cgatggccgc
     4561 tgcggctgcg aacgcaccca aactcggcac tatggtcggc tcgagcagca gatgcagcac
     4621 ggcgcagaga aacgttgaga cggcgcagcc gcgcgccagg acgcgggccg ccgcgccgcg
     4681 ccatttgagc cggaatagac aatacagtgc ccaacaaagg ccgctcagaa gagccaaccc
     4741 gataccggcg ggcgccagcg tcaaacgcgc gtctcccaac ag
//
LOCUS       DASWZT010000497         4072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_23573, whole genome shotgun sequence.
ACCESSION   DASWZT010000497 DASWZT010000000
VERSION     DASWZT010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23573"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VGE92_14235"
     CDS             <1..560
                     /locus_tag="VGE92_14235"
                     /inference="COORDINATES: protein motif:HMM:NF014124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RyR domain-containing protein"
                     /protein_id="HEY1315035.1"
                     /translation="LVPSRAPEPLKFSAAEIELLARLEHRRWSSERRLLGFTYGPVRS
                     QNPPRHELLVEWEQLPEKVRERNRADLGTLPDILAAANFEIRRDRKILAVGPTLNAAV
                     AELETAVAGQEKNIVVIADVDTEAGRKAAELTLKLPGSASWLVSSDYPQRLRESWPLG
                     PIWEGAAGWISREQLHNESAPKPAA"
     gene            complement(561..2498)
                     /locus_tag="VGE92_14240"
     CDS             complement(561..2498)
                     /locus_tag="VGE92_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315036.1"
                     /translation="MGPLPDRVPLVIGVTGHRDLRSQDIPRLEREIGAILTRLRQDYL
                     GGDSDTPLILLSALAEGADQIAARVALAQGIRLIAPLPMPLDEYRRDFDPGLAPGAAA
                     DFEKNLSQAIAAPVVPFTIGNSLEAVRADADKRTEQYRAVGLFIIQHCDVLIALWDGN
                     DNDRAAGGTSEMAAFKRQGIPLPVSGSAHASLDGSEIGPVIHVVTPRRKAGSPATEVA
                     VRRWGLDLAKHRRGDGIRRAMSRVAEFSAHLIGTERQDANSRLPPADRQDAEAWETFR
                     LLVTLTREFNNEAAAVADSADGSRRMAQSIDYLFTDPDDGRLDHDAKRRGFMSASHWC
                     RLYGIADTLAQYRQRQFKHDWQGLFVLGFVAFVLFALLIHFESADTVLLAAYTAFVAV
                     IYVVFFRARIMRHQEQFLDYRALAEALRVAVYWSILGIDEQKYAPGDGFDANTMGMLA
                     NSYPIKQPSELAWVKMCLRTLERMRDEGRTDAKASIDPTAHAIARRYWVANQLSYFGR
                     QGSRHNRRAEALEGWALILLFVTPFILVPLLLVTAFGRSQSMDPALHKFFLVIIGILP
                     GLAAALTGYSERLAMKAQARQYDRMRTLFGRAYDLLPETVDVASAPLVRSLYWELGTE
                     AMKENAEWVAIYRQRPIQPVQ"
     gene            complement(2522..3952)
                     /locus_tag="VGE92_14245"
     CDS             complement(2522..3952)
                     /locus_tag="VGE92_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315037.1"
                     /translation="MALWVRLAAKGGSVSAMLRQCGVRAFWVAVVALGLAACAIVDEY
                     SGRAIVYNLQAEQAQQQALLLNIVRASLRRPMQFTGLQTITGTASASGSLTAAGTSAS
                     QTPYISLFNLIPPAASSLVSRTITGNVGGTASMSGGPTFTVPVLDTQEFYQGLLNPIP
                     GQLLDLYLQAGYPRDVMFNLVIERIIIKKSGPGCPLDVHTPNCELTIRNYPPQDIDLE
                     LFQGVLGYLISLGLSTEPIIEPPVKQKAAPAGAAAQADATPKLKPYTFCFAPRDEKVY
                     KFFNRGVLCGHPAAGLLVKPSEIGRKSVISGVPIFQDFINRLYDETIQPRLGSDIGAD
                     GFRAIRSFGGQKVSISFNTRSIEEILYYLGEVGRRSLHPEAGREVRRVQVRVGPPQNQ
                     FPNRPCPFEEPINGYVCRDMFVLEEDSATQGGIAVKYEGTRYSISPDSSTAWSMPVLD
                     IVKQLLAVNTSAKQLPASNLISVLSN"
BASE COUNT          789 a         1225 c         1275 g          783 t
ORIGIN      
        1 gcctggtgcc gtcgcgggcg ccggaaccgc tcaagttcag cgccgcggag atcgaattgc
       61 tggcgcggct tgaacatcgc cgctggtcga gcgagcgtcg cttgctcggc tttacctatg
      121 gtccggtgcg ttcgcagaat ccgccgcggc atgagctgct ggtcgagtgg gaacagcttc
      181 cagaaaaagt gcgtgaacga aatcgcgccg atctcggcac gctgccggac attcttgcag
      241 cggcgaattt cgagatccgg cgcgaccgta aaatattggc agtcgggccg acgcttaatg
      301 ccgccgtggc ggagctggag accgcggtcg ccggccagga aaaaaacatc gtagtcatcg
      361 cggatgtcga caccgaggcc ggccgcaagg cggcggagct tacgctgaaa cttccgggtt
      421 cggcttcgtg gctggtttcc agcgactatc cgcaacgatt gcgcgaaagc tggccgctcg
      481 gaccgatttg ggaaggggcg gcggggtgga tttcgcgcga acagctgcac aacgaaagcg
      541 cgccgaagcc ggcggcctga ttattgcacc ggctggatcg gacgttgccg gtagatcgcg
      601 acccattcgg cattttcttt catcgcctcg gtgccgagtt cccaatagag cgaccgcacc
      661 agcggcgccg aagcgacatc cacggtttcc ggcaacaggt cgtaagcccg tccgaacagc
      721 gtccgcatgc ggtcgtattg ccgcgcctgc gccttcatcg ccagccgttc tgaatagccg
      781 gtcaacgcgg cggcgagacc cggcaggatc ccgatgatga ccaggaagaa tttgtgcaga
      841 gccgggtcca tcgactgact ccggccgaag gccgtgacca gcagcagcgg caccaaaata
      901 aacggcgtga cgaacagcag gatgagcgcc caaccttcca atgcttccgc gcggcgatta
      961 tgccgcgacc cctgccggcc gaaatatgag agctggttgg cgacccagta acgtcgtgcg
     1021 atagcatgcg ccgtgggatc gatgctggct ttggcgtcgg tgcggccttc gtcgcgcatc
     1081 cgttccagcg ttcgcaggca cattttgacc caggccaatt cgctcggctg cttgatcggg
     1141 tagctgtttg ccagcatccc catggtgttg gcgtcgaaac cgtcgccggg cgcatatttt
     1201 tgctcgtcaa ttccgagaat gctccaatag accgcgacgc gcagcgcctc agccagtgcg
     1261 cggtagtcga gaaactgttc ctgatgccgc atgatgcgcg cgcgaaaaaa tacgacatag
     1321 atcactgcga cgaaggcggt gtaggcggcc agcagcaccg tatcggccga ctcgaaatgg
     1381 atgagcaagg cgaacagtac aaaggcaacg aaaccgagca caaaaagccc ctgccaatcg
     1441 tgcttgaact gtctttgccg gtattgtgcc agtgtgtcgg cgatgccgta aagtcggcac
     1501 cagtgcgaag ccgacatgaa tccgcgccgc ttcgcgtcgt ggtcgaggcg gccatcatcc
     1561 ggatcggtga acagataatc gatgctttgg gccattcggc gcgaaccgtc cgcgctgtcc
     1621 gcgacggctg cggcttcgtt gttgaactcg cgcgtcagcg tgaccagcag cctgaatgtc
     1681 tcccatgctt cggcatcttg ccggtccgcg ggcggcaaac gcgagttcgc gtcttgccgt
     1741 tccgttccga tcagatgcgc ggaaaattcc gcgacgcggc tcattgctcg ccggatgccg
     1801 tcgccgcggc gatgcttggc gagatcgagg ccccagcggc ggaccgcaac ctcggtcgcc
     1861 gggctgccgg ctttgcggcg cggcgtcacg acatggatca ccggcccgat ctccgagccg
     1921 tcgagactgg cgtgcgccga tccactgacc ggcagcggaa ttccttgccg cttgaacgcc
     1981 gccatctcac tggtcccgcc ggcggcgcgg tcgttgtcgt tgccatccca aagcgcgatc
     2041 agcacgtcgc aatgctggat gatgaacagg ccaaccgcgc gatattgctc ggtgcgcttg
     2101 tccgcgtcgg cccgcaccgc ctcgagtgaa ttgcctatgg tgaagggcac gaccggcgca
     2161 gcaatcgcct gggaaagatt tttttcgaaa tcggctgcgg cgccgggcgc cagtcctggg
     2221 tcgaaatcgc gccgatattc atcaagcggc atcggcagcg gcgcgattag ccggatacct
     2281 tgcgccagcg cgacgcgcgc ggcgatctgg tcggcgcctt cggccagggc ggagagtaga
     2341 atgagcggcg tgtcgctgtc tccgccgagg tagtcctgcc gtagccgggt gagaatcgca
     2401 ccgatttcgc gctcaagccg tgggatgtcc tggctacgca agtcgcggtg tccggtgaca
     2461 ccgatcacca gcggcacgcg gtcgggcaaa ggccccatga acacggctcc agttgtctca
     2521 gttaattact gagaactgaa atgaggttgg aggcgggcaa ttgtttcgcc gacgtgttta
     2581 ccgccagcag ttgcttcaca atgtcgagta ccggcatgct ccatgcggta ctcgaatctg
     2641 gcgaaatgga gtagcgcgtg ccttcatatt tgaccgcgat gccgccctgc gtcgccgaat
     2701 cttcctcaag gacgaacatg tccctgcaga cgtagccgtt gataggctct tcaaacgggc
     2761 acgggcgatt tgggaattgg ttctgcggcg gaccgacccg aacctgcacg cgtcgcacct
     2821 cgcgccccgc ctccggatgc agcgaccggc ggccgacctc tccgagataa taaagaattt
     2881 cttcgattga ccgggtgttg aaggaaatcg agaccttctg gccgccgaac gagcggatgg
     2941 cacggaagcc gtcggcgccg atatcgcttc caagccgcgg ttgtattgtc tcgtcgtaga
     3001 gccggttgat aaaatcctga aatatcggca cgccactgat aacggacttg cgtccgattt
     3061 cggaaggttt caccagcaat cccgccgccg gatgcccgca aagcacgccg cgattgaaga
     3121 acttgtagac cttctcgtcg cgcggcgcga aacagaatgt gtatggtttc aatttcggcg
     3181 tggcatccgc ttgcgccgcg gccccggccg gagcggcttt ttgttttacc ggcggctcga
     3241 tgatgggctc ggtgctcaat ccgagactga tcaaatatcc tagtacgccc tggaagagct
     3301 caagatcgat atcttgcgga ggataatttc tgatcgtcaa ttcgcagttc ggcgtgtgca
     3361 catcgagtgg gcagcccggt ccgcttttct tgatgatgat ccgctcgata acgaggttga
     3421 acatcacgtc ccggggatat ccggcctgta aataaaggtc gagcaattgg ccggggatcg
     3481 ggttcagcag gccctgatag aattcctggg tgtcgagcac cggcaccgtg aaggtcgggc
     3541 cgccgctcat cgaagcggtg ccgcccacgt tccccgtaat cgtgcggctg accaaggagc
     3601 tggcggccgg cggaatcaaa ttaaacaacg agatgtacgg agtctgcgac gccgaggttc
     3661 cggcggcggt caggctgccg ctcgcgctgg ccgtgccggt gattgtctgc agtccggtga
     3721 attgcatcgg ccggcgcagc gacgcgcgga cgatattgag caacaaggcc tgctgctggg
     3781 cttgctcggc ctgcagatta tagacgatgg cccggccgga atattcgtcg acaatggcgc
     3841 atgccgctaa gcccaaggct acgacggcga cccagaacgc acgaacacca cactgacgca
     3901 acattgcgct tacggacccc ccttttgccg caagccgaac ccacaaagcc aagacagagt
     3961 agtgaccgga atctcatgtt gcaacagaca tttcggttgt tgccgaggcg ccaggtcaaa
     4021 ccggatttcg cgtggctact tcttgcgcac ctttttggca gatttggccg cg
//
LOCUS       DASWZT010000498         4584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_76470, whole genome shotgun sequence.
ACCESSION   DASWZT010000498 DASWZT010000000
VERSION     DASWZT010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76470"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..449)
                     /locus_tag="VGE92_14250"
     CDS             complement(60..449)
                     /locus_tag="VGE92_14250"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY1315038.1"
                     /translation="MFQSTAVQRLALAGSIIGCCAAACLAAAAPLAGDAAHGKILYQA
                     CSACHSIDENDLGPKHRGVVGRRAGNIADYAYSPALKQSGLTWDEAMLDRWLTNPSAL
                     VPGTKMFFKIDDAQSRADIIAYLKEQN"
     gene            complement(514..2157)
                     /locus_tag="VGE92_14255"
     CDS             complement(514..2157)
                     /locus_tag="VGE92_14255"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY1315039.1"
                     /translation="MPIDPLMTSVLEGLPDLVMLVRRDGVLLAHSGGRGVAALLPADP
                     SIGQNIETIWPATAAGVIRHLVRQALASRTTHDATFSEGERTYEARVCAQGPDRAICL
                     IRQPSVSAAEEQTGATGRFGAVALQFDRRSFLRRFHESLSMATLREQPTAVAVVHVDG
                     IVDVARVIDTHVAEQVMSAAIGRLTHERNLQAGPEIGWYVGQLSESQIVIVIESRDRD
                     RIEALIGGHCDSLREPIKVGDADFHLTPYAGVAILGPDATAPRRLLNHARGTAAEARR
                     AGSSHVCFFSDTVRLRSLARLDLAQELRDAIAERSIKLRYMARRDLTSGELLAAVGYV
                     RWHHPLRGEVSASEFLAVAETTGLATALSRAALKCLRDDYADLRRVGGPTLSLSFGPL
                     RHHILDDGFMDDLRELFSDGVLRPDRVELRISERSLFAADMSLLHAIVRLGVTLIVDE
                     VGRGIGAFDILARAPLAGLQIDRASVHSLAHDPSALKICRAGFALAQAIGLKSMARGV
                     DSAEQRDVLAGLGCSQGIGDLYGEINLADAAARRAAAAS"
     gene            2331..>4584
                     /locus_tag="VGE92_14260"
     CDS             2331..>4584
                     /locus_tag="VGE92_14260"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY1315040.1"
                     /translation="MKRLSEGFPAPFGTRLRVARRTFAIFAMSAVIPTLLGAAVAYQA
                     VRAELDSQMQKRLMQNARSYGLAVFTRLTAADQMLLQIAESTPASAALSQSIFTSVAI
                     RHRGAAVPATDGTRDAATLATAQAALQGTARDSTLVFERDDSQVVPVLIRRLTPAGEA
                     FVIGRMRTDYLWGDTEQLASDTQLCIRDRANTFNFCSQDGTERNSESPRVPAGNAPGA
                     LTTVSWPLYLRARFGIDPLYVSAGQPRILGLGALNSLRATLISVIVGTLALALLFGAF
                     EIRRTFRPLDVLLAAMSRLARGDFARAVVPPSAANGDFRELAASINSTAVTLGQQSHV
                     LSTLSQIDRKILTADSVETVIALVLPSIPAIAGCSAALLLTTHEDEGDGMFYSLAPSG
                     TLARRPCKLEMHALQRVVEEDAPLHLSDASPLPFAALREAGTRAVRTFPVRDGTALIG
                     ALVLLDPPDMPENAQPRHALDLADRLSVAISHARSKQDLLRNAYFDPLTGLANRELFC
                     DRIGQALGRDGKRRGAAVLLIGIDRFKNINDTLGHRAGDELLKLTAERLRAVIGTGDT
                     VARLIGDEFIVLTSDLAAPGRVVRLAELIESSFSKPFLADGIGYVLSVSIGIAIAPGD
                     GLTPDVLIRNADIAMRRAKTRSVGAVVFFEETMNEQAKRRFRLEHELRAALEQGEIEV
                     HFQPKIELKTGRVIASEALLRWTHPSDGAIPPAVFIPIAEETGLIVPLGYWVIEEACR
                     HLIQWRAAGLG"
BASE COUNT          799 a         1525 c         1470 g          790 t
ORIGIN      
        1 gaccgaagac cgcaccgagc gcgttctggc gctgtcgctc gactatcgct ggtaacgagt
       61 caattctgct ctttgaggta cgcgatgatg tccgcgcggc tctgcgcgtc gtcgatcttg
      121 aagaacatct tcgtccccgg aacgagtgca ctcggattgg tcagccagcg gtcgagcatg
      181 gcctcatccc aggtgaggcc cgattgcttg agggccggcg agtacgcgta atctgcgatg
      241 tttcccgcac gccgcccgac caccccgcgg tgcttgggac cgagatcatt ctcatcgatg
      301 gaatgacagg cactgcatgc ctgatacaag atcttgccgt gcgccgcatc gccggcgaga
      361 ggcgcagcgg cggcgaggca cgcggcggcg caacatccga tgatcgaacc ggccagggct
      421 aggcgctgaa ccgctgttga ctggaacacg tcgtctcccc aattgaacac tcaccgggaa
      481 gacttcgctg cgcgtgcttt tattcccttg cggctatgag gccgccgccg cccgccgcgc
      541 ggccgcgtcc gcgaggttga tctccccgta caaatcgccg atgccctggc tgcacccgag
      601 tcccgccaag acatcgcgct gctcggcaga atccacgccg cgagccatcg atttcagacc
      661 aatcgcctgg gccagcgcaa atccggcgcg gcaaatcttg agtgcgcttg gatcatgcgc
      721 caagctgtgg accgaggcgc gatcgatctg caaaccggcc aacggcgcac gcgccaggat
      781 gtcgaatgca ccgatgccgc gcccgacctc atcgacgatc agcgtcaccc cgaggcgcac
      841 gatcgcgtgc agtaacgaca tatcggcggc aaacaacgag cgctcgctga tccgcagctc
      901 cacccgatcc gggcgcaata cgccgtcgct gaacagctcg cgcagatcat ccataaagcc
      961 gtcgtcgagt atgtgatgcc gcagcggccc gaatgacagg ctcaaggtag gaccgccgac
     1021 gcgccgaagg tcggcgtagt cgtcccgcag gcacttgagg gcggcgcgcg acaacgccgt
     1081 ggcgagaccg gtcgtctcgg ccaccgcaag aaattccgaa gcgctgacct cgccgcgcaa
     1141 cggatggtgc caacgaacgt agccaaccgc cgcgagcagt tcgccgctcg tcagatcgcg
     1201 ccgcgccatg taacgcagct tgatcgagcg ctcggcgatg gcatcacgca actcctgcgc
     1261 gagatccaag cgggccagag accgcaaacg caccgtgtcg gagaaaaaac acacgtggct
     1321 gctcccggcg cgacgcgctt cggctgcagt cccgcgcgcg tggttgagca gccgccgcgg
     1381 cgccgtggca tcggggccca agatcgcgac tccggcatac ggcgtcaaat gaaaatccgc
     1441 gtcgccgacc ttgatcggct cgcgcaagct gtcgcagtgg ccgccgatga gcgcctcgat
     1501 gcgatcacga tcgcggctct cgatcacgat gacgatttga ctctcgctca gctggccgac
     1561 gtaccacccg atctcggggc cggcttgcag gttgcgctcg tgcgtcaatc ggccgattgc
     1621 cgcactcatc acctgctccg cgacatgcgt gtcgataacg cgcgcgacat cgacgatgcc
     1681 gtcgacgtga actacggcga cggcggtcgg ctgttcgcgc agcgtcgcca tcgacaggga
     1741 ttcgtgaaag cggcgcaaaa aggagcgccg atcgaattgc agggcgactg cgccgaagcg
     1801 gccggtggca ccggtttgtt cctcggctgc ggaaaccgac ggctggcgga tcaggcagat
     1861 ggcgcggtcc ggcccctgcg cgcaaacccg cgcctcatag gtgcgctcgc cctcgctgaa
     1921 tgtcgcatca tgcgtcgtgc ggctggccag ggcttggcgc accaggtggc gaatcacccc
     1981 ggccgcggtc gccggccaaa tggtctcgat attttggccg atcgagggat ccgccggcaa
     2041 taaggccgcg acgccccgtc cgccgctatg cgccaaaagc acgccatcgc gccgcaccag
     2101 catcaccagg tccggcagtc cttcgagcac tgaggtcatg agtgggtcga ttggcatgga
     2161 gtcgtgaact gtatcgggcg cagtttcgag gaaccggacc gcaatcacgg tttgagggtc
     2221 tgaatccggg ctaacgtagg gctggtaagt ggcggaaggt ccgcacgctc gccgtgtcgg
     2281 cctcggggct ttcatggctg agcgcccgta gtaactcagg acgaaatggg atgaagcggc
     2341 tcagcgaggg atttccggcc ccgttcggaa cgcgtctgcg tgttgcgcgg cgtacgttcg
     2401 caatcttcgc aatgagcgcg gtgattccga cgttgctcgg cgccgcggtc gcctatcagg
     2461 cggtacgcgc cgagctcgac tcgcaaatgc aaaagcgtct catgcagaac gcacgcagct
     2521 acgggctcgc ggtgttcacg cgcctgacgg ccgccgatca gatgctgctg cagatcgccg
     2581 aatcgacacc ggccagtgcg gcgctgtcac aatcgatttt tacctcggtc gcgatccgac
     2641 atcgcggcgc agccgtgccg gcgacggacg ggacgcgcga cgcagcgacg ctcgcgaccg
     2701 cgcaagccgc actccagggc accgcccggg attcgaccct ggtgttcgag cgcgacgatt
     2761 cgcaagtggt gcccgtgctg atcaggcgcc tcacaccggc gggcgaggcg tttgtcatcg
     2821 gccgcatgcg caccgattat ctgtggggcg acaccgagca gctcgcgagc gacacgcagc
     2881 tctgcatccg cgaccgagcc aatacgttca acttctgctc tcaggacggc accgaacgaa
     2941 acagcgagag cccccgcgtg cctgccggga acgccccagg tgcgctcacc acggtcagtt
     3001 ggccgctgta cctgcgcgca cgcttcggga tcgatccttt gtacgtgtcg gccggccagc
     3061 cgcgcatcct cggccttggc gcattgaatt cgctgcgcgc gacgctcatt tccgtgatcg
     3121 tggggacgtt ggcgctcgcc ttgctgttcg gcgcgttcga gattcgccgc acctttcgcc
     3181 cgctcgatgt attgctcgcc gccatgagcc ggctcgcgcg cggcgacttc gcacgagcgg
     3241 tggtgccgcc gtcggcggcc aacggcgact tccgtgaact cgccgcctcg atcaattcaa
     3301 ccgccgtcac gctcggccag caatcgcatg tcttgagtac cttgagccaa atcgaccgca
     3361 agatcctcac ggcggattcc gttgaaaccg tgatcgccct ggtgctgccg agcattcccg
     3421 ccattgcggg ctgcagcgcg gcgctcttgt tgacgaccca cgaagacgag ggcgacggca
     3481 tgttctattc gctcgcgccg agcggcacgc tggcgcgtcg gccgtgcaag ctcgagatgc
     3541 acgcattgca acgcgtcgtc gaagaggatg cgccgctgca cttgtccgac gcctcgccct
     3601 tgcccttcgc ggcgctgcgc gaggcgggta ctcgcgcagt gcgaaccttt ccggtgcgcg
     3661 acggcaccgc cctcattggc gccctggtgc tgctcgatcc gccggacatg ccggaaaacg
     3721 cgcagccgcg tcatgcgttg gatctcgccg atcgcctctc ggttgcaatc tcgcacgcgc
     3781 gcagcaagca ggatctgttg cgcaatgcgt acttcgatcc actcaccggg ctcgccaatc
     3841 gcgagctgtt ctgcgatcgc atcggccagg cgctcggtcg cgatggcaaa cgccgcggcg
     3901 ccgccgtgct gttgatcggc atcgaccgtt tcaagaacat caacgacacg ctcgggcacc
     3961 gtgccggcga cgaattgctg aaactgaccg cagagcggct gcgcgccgtc atcggcacgg
     4021 gcgatacggt ggcgcggctg attggcgatg aatttatcgt cctgacgtcc gacctcgcgg
     4081 ctccgggccg ggtcgtgaga ctggcggagc tgatcgagag ctcgttctcc aagccgtttc
     4141 tcgccgatgg aatcggctac gtgctgtcgg tgtcgatcgg gatcgcgatt gcgccgggcg
     4201 acgggcttac gcccgacgtg ttgattcgca atgcggacat cgcgatgcgc cgcgcaaaga
     4261 cccgcagcgt cggcgccgtg gtctttttcg aggagaccat gaacgagcag gcgaagcgcc
     4321 gttttcgcct ggaacatgaa ctgcgcgcgg cgctcgagca aggcgaaatc gaggtgcatt
     4381 ttcagccgaa gatcgaactg aagaccgggc gggtcatcgc cagcgaggcg ttgctgcgct
     4441 ggacgcatcc gagcgatggc gcaattcctc ccgccgtctt cattcccatc gccgaggaga
     4501 ccggactcat cgtgccgctc ggttattggg tgatcgagga ggcgtgccgt caccttatcc
     4561 aatggcgggc cgccgggctc ggcg
//
LOCUS       DASWZT010000499         9523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_23574, whole genome shotgun sequence.
ACCESSION   DASWZT010000499 DASWZT010000000
VERSION     DASWZT010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9523
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23574"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1411
                     /locus_tag="VGE92_14265"
     CDS             <1..1411
                     /locus_tag="VGE92_14265"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEY1315041.1"
                     /translation="GVLKRLYPSIYTSNVDSPPESIVLRHWQAIVGHLLPGGVISHRS
                     AFDSRPFEGRLSITRGKTRRDVRLPGLTIHIVPGPGPRLEPPAPDSRFGELYLASDPR
                     RFLENLTRGRGWSDRVLPQPALEAALDRILLVGGPKRLNQLRDHAREIAPSLGYDAQF
                     KRLNNLIGALLGTHESKILTAKQALARAAGRPYDPHRLEIFDALFSALNQAAFESIED
                     PAPTGVAREDFAFFEAYFSNYIEGTTFEVEEAESIIFEGKIIENRNEDSHDILGTFQA
                     ATTAPWRDRPPQNADEFLVWLQNVNALVMQKRADRKPGEWKEKANQAGSTYFVVPELV
                     VGSLREGFERIRALPHPLAQAIMTMFVVAEVHPFSDGNGRTARLAMNCMLSARALSRI
                     IIPTVYREDYLLPLRALSHNRIAEPLISALSRVQRWSAAFDYSLPRARLRESLSRCNA
                     FQEDLRSYKLLFPTAKLVD"
     gene            complement(1548..1623)
                     /locus_tag="VGE92_14270"
     tRNA            complement(1548..1623)
                     /locus_tag="VGE92_14270"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1588..1590),aa:Lys,seq:ttt)
     gene            complement(1677..2372)
                     /gene="queC"
                     /locus_tag="VGE92_14275"
     CDS             complement(1677..2372)
                     /gene="queC"
                     /locus_tag="VGE92_14275"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005399706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEY1315042.1"
                     /translation="MNAAGSSAGPSRAIVLLSGGLDSSTVLALACAQGFECYALSVHY
                     GQRHSAELTAARRIAAALGAREHRIMGVDLAGIGGSALTDSRVAVPETPQAGIPVTYV
                     PARNSLLLSLALGWAEVVDAADIFVGVNAVDYSGYPDCRPEFIEAFERLARLATKAGV
                     EGKDFKIQAPLIALSKAEIIRAGADLGVDFAMTVSCYQATEDGAACGKCDSCRLRAAG
                     FAAADIADPTRYR"
     gene            complement(2369..3028)
                     /gene="queE"
                     /locus_tag="VGE92_14280"
     CDS             complement(2369..3028)
                     /gene="queE"
                     /locus_tag="VGE92_14280"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016711990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEY1315043.1"
                     /translation="MGAAVKSDRLRVNEIFHSLQGEADAVGYPTVFVRLTGCPLRCRY
                     CDTEYAFHAGEWLDLESIVEKVRTYGARHVCVTGGEPLAQPNCLKLLARLCDHGFQVS
                     LETSGALDIAAVDPRVSRVVDVKTPGSNEAARNRIENFDLLTPKDQLKFVICSRQDYD
                     WSKAFLQEHGLPEQCRILFSPSYNEVSPTALADWILSDRLPVRFQLQLHKILWGDIPG
                     K"
     gene            complement(3028..3819)
                     /gene="ybgF"
                     /locus_tag="VGE92_14285"
     CDS             complement(3028..3819)
                     /gene="ybgF"
                     /locus_tag="VGE92_14285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEY1315044.1"
                     /translation="MISRAALALRTGCLVGLALAGCASTPEEDPVQIKLNDLDTRLTR
                     IERVVSNQSLLDLANQNESLRNDLRAMHNDVDQLTNSLEASRKQQRDLYADLDRRMKL
                     LESRGGAAGNGDTAAAAGAGGSGAAGDTAAGAGSDDKTAYQAAFTLLKEGQYDRAIPA
                     FQKFLIVYPDSQLADNAQYWLGEAYYVNKSFPEAQAAFQRVIDKYPQSRKRPDALLKI
                     GYCHYELKEWEAAKETLGKVSAQFPETPAGHLAQQRLDKMATEQH"
     gene            complement(3816..4352)
                     /gene="pal"
                     /locus_tag="VGE92_14290"
     CDS             complement(3816..4352)
                     /gene="pal"
                     /locus_tag="VGE92_14290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEY1315045.1"
                     /translation="MKRIGSIALLMVAAALLSGCPKKHNVNEPPAAGTQVPGSSSSDS
                     ASTQARALDDSANQANGMANATGVLARRVIYFDFDKSEIKPEFADLVAAHARNLTSHP
                     NMRIKLEGNTDERGTREYNIGLGERRAQAVRRALMLQGVAESQLSTVSFGAERPAVEG
                     DDEAAWAKNRRVELVYGP"
     gene            complement(4433..5731)
                     /gene="tolB"
                     /locus_tag="VGE92_14295"
     CDS             complement(4433..5731)
                     /gene="tolB"
                     /locus_tag="VGE92_14295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEY1315046.1"
                     /translation="MNKHVSTVVAIGLLLFCAESRAELVVEITKGQADAIPIAIVPFS
                     PPEAAAASFDVAQLVSDDLARSGRFRSTDRKDMIEQPHAGAAISFDDWRRLNNDYIVV
                     GQVQPAGVDHYNITFELYNVLNHQRLLAYQISANKPGLRLASHQVADMVFEKILGIRG
                     AFATRIAYISVLGALPHKSYRLIVADADGENPHVVMQSNEPLMSPAWSPDGQSLAYVS
                     FEDRLPSVYVQLLKTGERRRVSAQAGVNQAPAWSPDGKKLALTLSTRDGNLDVYVLDL
                     TTQALTRITDDPGIDTEPQWSKDGQSLYFTSDRAGGPQIYRVGTQAGDKPRRLTFQGS
                     YNARPRLSPDESQLAFVTQEDGGYHIATMDLRGRGDTQVLTKGRFDVSPSYAPNGAEI
                     IYASRDRGRGVLALVSADGRVQERLVSSEGELQEPAWAPF"
     gene            complement(5724..6662)
                     /gene="tolA"
                     /locus_tag="VGE92_14300"
     CDS             complement(5724..6662)
                     /gene="tolA"
                     /locus_tag="VGE92_14300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HEY1315047.1"
                     /translation="MRNFLREHAWGVVVSVLLHGLLIGGLVFASFNFMKPTPPSLEPL
                     PIDAVVVDSQVLHAAQRAQDEARAAAAAAAQAQAAAAARAAEDAKAQEDKAAADANAE
                     AAAKAAADANAAADQQAAEHAQAQAAEQQHLAEVQKAGAAQKAAAARRAAEEDLAEQE
                     KRALQAKKAAEAQAAEAKQAAEAKRIADAKRAADDRARADREAELRRQLADEEHRSQV
                     ESGPLRDSYIAKLRNTIQNAWIKPPSASVGLDCLVEVTQIPGGEVTGARVTQCNGDQA
                     ARQSIENAVYRASPLPEPPDPALFERNFVFRFKPNE"
     gene            complement(6663..7139)
                     /gene="tolR"
                     /locus_tag="VGE92_14305"
     CDS             complement(6663..7139)
                     /gene="tolR"
                     /locus_tag="VGE92_14305"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HEY1315048.1"
                     /translation="MPAPLRRRKLMGEINVVPYIDVMLVLLVIFMITAPLLKEGVKVD
                     LPEAGAKPIEPEFLTKHEPLVVTIDARGRLYVNFGKDSDKPASEDTVSARTAALLRRD
                     PQTPVLVKADTTVPYGTLMRAMVLLQHAGATKVGFLTDPIRPPPRGQANGAGSGPG"
     gene            complement(7145..7831)
                     /gene="tolQ"
                     /locus_tag="VGE92_14310"
     CDS             complement(7145..7831)
                     /gene="tolQ"
                     /locus_tag="VGE92_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEY1315049.1"
                     /translation="MGNDLSIWTLVLDASVIVQAVMVLLMVASVASWAIIFKKSRVIS
                     HSRRQADQFETAFWSGGDLATLYRSIETKGREGTGEQSIFESGFGEFSRLRQQGAPTD
                     QLLEGSRRAMRVAQMREMDRLERNLATLATVGSTSPYVGLFGTVWGIMSAFHNLGNVQ
                     QATLAAVAPGIAEALIATAIGLFAAIPAVIAYNRFADQVSRLELRFDTFVEEFSTILQ
                     RHGPSRAAQA"
     gene            complement(7821..8231)
                     /gene="ybgC"
                     /locus_tag="VGE92_14315"
     CDS             complement(7821..8231)
                     /gene="ybgC"
                     /locus_tag="VGE92_14315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HEY1315050.1"
                     /translation="MPVRRFSIECRVYWEDTDAGGVVYYANYLKFMERCRSDWLRALN
                     IDQQRLRAERQLQFVVVNVSVDFLRPAVLNDEIRVSAELERLSGATILFEQTIARGDV
                     QLIDASVRVACLDSGTLKPRPIPKDLFVEWRNGE"
     gene            complement(8232..9281)
                     /gene="ruvB"
                     /locus_tag="VGE92_14320"
     CDS             complement(8232..9281)
                     /gene="ruvB"
                     /locus_tag="VGE92_14320"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEY1315051.1"
                     /translation="MGIEQDRIIAGPVKREDEVLDRAVRPKSLADYVGQSSVKTQMDI
                     FIQAARNRGEALDHVLIFGPPGLGKTTLANIIANELKVNLRQTSGPVLERPGDLAALL
                     TNLEPRDVLFVDEIHRLSPVVEEVLYPAMEDSQLDLMIGEGPGARSIKLDLPPFTLVG
                     ATTRAGLLTSPLRDRFGIVQRLEFYSTQDLTSIVTRSAAILKLQLQADGAVRIAERSR
                     GTPRIANRLLRRVRDFAEVKAAGIISAQVAIAALDMLEVDPLGFDMLDRKLLLTVIEK
                     FDGGPVGVDSLAAAMSEERGTIEDVIEPFLIQQGFLMRTARGRVATRSAYLHFGLPAP
                     ALERDLNLPLFRDAQ"
     gene            complement(9290..>9523)
                     /locus_tag="VGE92_14325"
     CDS             complement(9290..>9523)
                     /locus_tag="VGE92_14325"
                     /inference="COORDINATES: protein motif:HMM:NF019140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315052.1"
                     /translation="RVIIEMRDSVKKFSTPAPGDSLPGAGPAATAQSEAFSALIALGY
                     KPPEVVRLLKTVDEPDLATTEIIRRALKSAVKA"
BASE COUNT         1784 a         3056 c         2832 g         1851 t
ORIGIN      
        1 gggtgtactc aagcgattgt acccgagcat ctacacgtcc aacgtagaca gcccgccaga
       61 atccatcgtc ttgcggcact ggcaggcgat cgtcggacat ctgctgccgg gcggtgtgat
      121 ctcgcatcgt tcagcattcg acagccggcc cttcgagggc cgcctctcca tcacccgcgg
      181 caagacccga cgcgacgtgc gattgccggg gttgacgata cacattgtgc cgggtcccgg
      241 cccccggctt gaacctcctg cgccggattc gcgcttcggc gagctgtatc tggcgagtga
      301 tccgcggcga ttcttggaaa atcttacccg cggccgcggt tggagtgatc gcgtgctgcc
      361 gcaaccggcc ctcgaggcgg cgcttgaccg cattctgctg gtcggcggcc ccaaacgcct
      421 aaaccaattg cgcgatcacg cgcgcgaaat cgctccatct ctgggttacg acgcgcaatt
      481 caagcgcctc aacaacctca tcggcgcgct gctcggtacg cacgaatcaa aaatcttgac
      541 cgccaagcag gccttagcgc gggctgcggg ccgtccctac gatcctcatc ggctggaaat
      601 cttcgatgcg ctgttcagtg cgctcaatca agccgcattc gaaagcatcg aggatcccgc
      661 gcccacgggc gtcgcgcgcg aggacttcgc gttctttgag gcgtactttt ccaactacat
      721 cgaggggacg acgttcgagg tcgaggaggc ggaatccatc atcttcgagg ggaagatcat
      781 cgagaaccgc aatgaagact cgcacgacat cttgggcacg ttccaagcag cgacgaccgc
      841 cccctggcgg gaccgtccgc cgcaaaacgc cgatgaattt cttgtgtggc tgcagaacgt
      901 caacgcgctc gtcatgcaaa agcgggcgga cagaaaaccc ggagaatgga aagaaaaagc
      961 caatcaggcc ggcagcactt acttcgtcgt accggaactt gtcgtcggga gcttgcggga
     1021 aggattcgaa cggatacgcg ccctgcccca tccgctcgcc caggccatca tgaccatgtt
     1081 tgtggtagcc gaagtgcacc cattcagcga cggcaacggc cgcacggcgc ggctcgccat
     1141 gaattgcatg ttgagcgcgc gcgctttgag ccgaatcatc attccgacgg tctaccggga
     1201 agactacctg ctgcccttga gggcgttgag ccacaaccgc atcgccgaac cgctcataag
     1261 tgccttgagc cgcgtacagc gctggtctgc agccttcgac tattcgttac cgcgagcgcg
     1321 attgcgcgaa tcgctttcgc gctgcaatgc ctttcaagaa gatctgcgca gctacaaatt
     1381 gctttttccg accgcgaagc tggtcgactg agccgcagac gattgggatg gctgtcgagg
     1441 agcggtccag gacgtctgcg gacgataaag acgctgcttt tacagggatt tgtcgctcga
     1501 ttggacgcct tggcggatct tagcggaagt cgtcggaagt gggggaatgg tgggacggct
     1561 gggactcgaa cccaggacca aagccttaaa agggccttgc tctaccaact gagctaccgt
     1621 cccaatcagc cgcgcatcat agccgaactg cgaggccagt tcatcggcat tctcattcac
     1681 cgatatcgcg tggggtcggc gatatccgcg gccgcaaagc ccgcggcacg caagcggcaa
     1741 gaatcacatt taccgcatgc ggcgccgtcc tccgtcgcct gataacagga caccgtcatg
     1801 gcgaaatcca cgccgaggtc cgcgccggca cggatgatct ccgccttgga cagggcgatc
     1861 agcggagctt ggatcttgaa gtcctttccc tcgactccgg ccttggtggc gagtcgcgcc
     1921 aagcgttcga aggcctcaat gaattccggc cggcagtccg gatatcccga gtagtcaacc
     1981 gcgttcaccc cgacaaaaat atcggcggca tcgaccacct ccgcccaacc cagcgccagc
     2041 gacaacagca ggctattgcg agccggcaca tacgtgaccg ggatgcccgc ttgaggcgtc
     2101 tcgggcacgg cgacgcggct atcggtgagt gccgatccgc cgataccggc gagatccaca
     2161 cccatgatgc gatgctcccg agcgccgagc gcagctgcga ttcgccgcgc ggccgtcaac
     2221 tcggcgctgt ggcgttggcc gtagtgcacg ctcaaggcgt agcactcgaa accctgcgcg
     2281 cacgcgagag caagcactgt cgaggaatcc aggccgcctg agagcaggac gatcgcccgg
     2341 ctgggacccg cggatgagcc ggcggcgttc acttgccggg tatatcgccc cacaagattt
     2401 tgtgaagctg caattggaag cgcaccggca ggcgatcaga gaggatccag tccgccaacg
     2461 cagtgggcga cacttcgttg tagcttggag agaacaggat tctgcattgc tccggcagac
     2521 cgtgctcctg caagaacgct ttgctccagt cgtagtcttg ccgcgagcag atcacgaatt
     2581 tcagttgatc cttgggcgtc aacaaatcga agttttcgat gcgattgcgc gccgcctcat
     2641 tcgagcccgg agtctttaca tcgaccacgc gcgacactcg aggatcgacc gcggcgatat
     2701 cgagcgcccc gctggtttcc agcgacactt gaaagccgtg gtcgcacagc cgcgcgagca
     2761 atttcaagca attcggctgt gccaagggct caccgccggt cacgcaaacg tgccgcgcac
     2821 cgtaggtgcg caccttctct acgatgctct cgaggtcaag ccattcgccc gcgtgaaagg
     2881 catattccgt gtcgcaataa cggcaacgca gtgggcaccc ggtcaggcgc acgaacaccg
     2941 tcggatagcc gaccgcgtcg gcttcccctt gcaatgagtg gaagatctca ttgacgcgca
     3001 ggcgatcgga tttgacagca gcgccaatca atgttgctcg gtcgccattt tgtcgaggcg
     3061 ctgctgggcc agatgtccgg ccggtgtctc ggggaattgt gcgctgacct tgccaagagt
     3121 ctccttggca gcctcccact ccttcaactc gtaatggcag tagccgattt tcagcaatgc
     3181 gtcgggacgt ttgcgcgatt gcggatactt gtcgatcacg cgttggaacg cagcctgcgc
     3241 ctcggggaag gacttattca cataatacgc ttcgcccagc caatactgtg cgttgtcggc
     3301 cagttggctg tccggataca cgatcagaaa cttttgaaac gccgggatcg cgcgatcgta
     3361 ttggccttcc ttcaacaacg tgaacgccgc ttgatacgcc gtcttgtcgt ccgatcccgc
     3421 ccccgccgcg gtatcgccgg cggcgcccga gccgcccgct cccgccgcag cggcggtatc
     3481 gccgttgccc gctgcgccgc cgcgcgactc gagaagcttc atgcgccggt ccagatccgc
     3541 atacaaatcg cgctgctgct tgcggctcgc ctcgagacta ttggtcaact gatcgacatc
     3601 gttgtgcatg gcgcgcaaat cgttgcgcag cgactcattt tgattcgcca aatcgagcag
     3661 gctttgattc gaaaccacgc gctcaatgcg agtcaggcgg gtatcgagat cgttcaactt
     3721 gatctgcacc ggatcttctt ccggcgtgct ggcgcaaccc gccaacgcca ggccgaccag
     3781 gcagcccgtc ctcaaagcga gcgctgcgcg ggaaatcacg gaccgtatac caattccacg
     3841 cgccgattct tcgcccatgc cgcctcgtca tcaccttcga ccgccggccg ctccgctccg
     3901 aagctcaccg tcgacagttg cgactccgcc acgccctgaa gcatgagtgc gcgccgcacc
     3961 gcctgcgcac gacgttcgcc gagcccaatg ttgtattcgc gagtaccgcg ttcgtcggtg
     4021 ttgccctcga gcttgatcct catgttcgga tggctggtca gattgcgcgc atgagccgcc
     4081 acgaggtcgg cgaactcggg cttgatctcg gacttgtcga agtcgaaata aatcaccctg
     4141 cgggccaaca cgccggtcgc gttcgccatg ccgttcgcct gattggcgga atcgtccaag
     4201 gcgcgcgcct gtgtcgaggc agagtcggag gaggagctac cgggaacttg tgtgcctgca
     4261 gcaggcggtt cgttcacgtt gtgctttttc ggacatcccg agagcagcgc cgcggccacc
     4321 atcagcaaag cgatcgaccc tattcgtttc atttaattct ccatccgaat gacttaaccc
     4381 gttagcctac cgatgaacac gagatttgtg tactttgttt ggctgcaggt gcttagaacg
     4441 gagcccacgc aggctcctgc aactcacctt cgctcgatac cagccgctcc tgtacccggc
     4501 cgtcggcact gaccaaggcg agcacaccgc gcccacggtc gcggcttgcg taaattattt
     4561 ccgcaccatt cggggcgtaa ctcggcgaca catcgaagcg gcccttggtg aggacttggg
     4621 tgtcgccgcg gccgcgtagg tccatggttg ctatgtgata gccgccgtcc tcctgggtta
     4681 cgaaggccag ttgggactcg tccggagaga gccgcggacg ggcgttgtag ctgccttgaa
     4741 acgtcagccg gcgaggttta tcgcccgctt gcgtgcccac gcgatagatc tgcggaccgc
     4801 cggcgcgatc cgatgtgaag tacaggcttt gaccgtcctt ggaccattgc ggctcggtgt
     4861 cgattcccgg atcgtcggtg atgcgagtca aggcctgcgt cgtcagatcg agcacgtaga
     4921 catccaaatt gccgtcgcgg gtcgacagcg tcagcgcgag cttcttgccg tccggcgacc
     4981 atgccggcgc ttgattgaca ccggcttgag ccgagacgcg ccgccgctct ccggttttca
     5041 gcaactgcac atacacggaa ggcaaccgat cttcgaacga cacgtacgcc aagctctgac
     5101 cgtcgggtga ccacgcgggc gacatgagcg gttcgttcga ttgcatcacg acatgcgggt
     5161 tctcgccgtc cgcatcggcc acgatcagcc ggtagctctt gtgcggcaac gcacccagca
     5221 cggaaatata agcgatgcgc gtcgcgaagg cgccgcgtat tcccaagatt ttctcgaaca
     5281 ccatgtccgc aacctggtga ctcgcgagcc gcaagccagg tttgttcgcg ctgatttggt
     5341 aagccaacag ccgctggtga ttgaggacgt tatacagctc gaaggtgatg ttgtagtgat
     5401 cgacgcctgc gggctgcacc tggccgacca cgatgtagtc attgttcagc cgccgccaat
     5461 catcgaacga gattgccgca ccggcatgcg gctgctcgat catgtccttg cgatcggtgc
     5521 tgcggaagcg gccgctgcgc gcgagatcgt cgctcaccaa ctgcgccacg tcaaaggatg
     5581 cggcggccgc ctccggcggg ctgaacggca cgatggcgat cggaatcgca tccgcctgac
     5641 ctttggtgat ttcgaccacc agctccgccc ggctctccgc gcagaacaac aaaaggccga
     5701 tcgcaaccac cgtcgatacg tgtttattca ttgggcttga acctaaatac aaagtttctt
     5761 tcaaaaagcg ctggatccgg gggttccggc agcggcgagg cacgatacac cgcattttca
     5821 atcgactggc gggcggcttg atcgccgttg cactgggtta cacgcgcacc ggtcacctcg
     5881 ccccccggaa tctgggtgac ttcgaccaaa caatcaaggc ccacgctcgc ggacggcggt
     5941 ttgatccagg cattctggat ggtgttgcgc agtttcgcga tgtagctgtc gcgcaaaggg
     6001 ccggactcga cttggctgcg atgctcctcg tccgccaact gcctgcgcag ctccgcctca
     6061 cgatcagcgc gtgcgcgatc atcggccgct cgtttggcgt cggctatccg cttggcctcg
     6121 gcggcctgtt tcgcctccgc agcctgggct tcggcggcct tcttcgcctg cagcgcgcgt
     6181 ttctcctgtt cggcgagatc ttcctcggcg gcgcggcgcg ccgccgccgc tttctgcgcc
     6241 gcgccggcct tttgcacctc ggcgagatgc tgctgctccg ccgcttgagc ctgggcgtgc
     6301 tcggccgcct gttggtctgc agcggcattg gcatctgcag ccgctttcgc cgctgcctcg
     6361 gcgttggcat ctgcggcggc cttgtcctct tgggccttgg cgtcctcggc cgccctcgcc
     6421 gctgcggctg cctgcgcctg tgcggccgca gcagcggcgg cacgcgcttc gtcttgtgca
     6481 cgttgtgcgg catgcagaac ttgcgaatcg acgaccacgg cgtcgatcgg caagggctcg
     6541 aggctcgggg gtgtcggttt catgaaattg aacgatgcga acaccaggcc gccgatgagc
     6601 aatccgtgca gcagcacgga aaccacgact ccccacgcat gctcgcgtaa gaaattgcgc
     6661 attcaacccg ggccgctgcc ggctccgttg gcttgccccc gcggcggcgg ccgtatcgga
     6721 tcggtgagaa acccgacctt cgtcgcgccg gcgtgctgca gcaacaccat cgcccgcatc
     6781 agggtgccat acggcaccgt ggtgtcggct ttgaccaaca ccggggtctg cggatcgcgg
     6841 cgcagaagcg cagccgttcg cgcgcttacc gtgtcttcgg atgccggctt gtcggaatcc
     6901 ttcccgaaat tcacatagag acgtccgcgg gcgtcgatgg tcacgaccag gggttcatgt
     6961 ttggtgagaa attcgggttc gatgggcttg gcgccggcct ccggcaaatc caccttgaca
     7021 ccctctttga gcagcggcgc ggtgatcatg aaaatgacca gcaataccaa catgacgtcg
     7081 atgtacggca ccacgttgat ctctcccatc agcttgcggc ggcgaagcgg tgcgggcatg
     7141 ggtgtcaggc ttgagccgcg cgggacggcc cgtgacgctg cagaatcgtc gagaactctt
     7201 cgacgaaggt gtcgaacctc agttccaagc ggctcacctg atcggcaaac cggttgtagg
     7261 cgatcaccgc gggaatcgcg gcgaacaagc cgatcgccgt ggcgatcagc gcctcggcaa
     7321 tacccggtgc caccgccgcc aaggtagcct gttgaacgtt gccgaggtta tggaatgcac
     7381 tcatgatgcc ccacaccgtg ccgaacagac ccacgtacgg actcgtggaa ccgaccgtcg
     7441 ccagtgtcgc aagattgcgc tcgagccgat ccatctcgcg catttgcgca acgcgcatcg
     7501 cacggcgcga tccctcgagc agctgatcgg tcggcgcgcc ttgttggcgc agccggctga
     7561 actcaccgaa tcccgattcg aaaatacttt gctcgccggt tccttcacgc cccttggttt
     7621 cgatgctgcg gtacagagtc gcgagatccc cgcccgacca aaaggccgtc tcgaactgat
     7681 cggcctggcg tcgcgagtgg gaaatcacgc ggctcttctt gaaaatgatg gcccacgagg
     7741 ccaccgatgc caccatcagc aataccataa cggcctgcac gatgacgctc gcgtcgagca
     7801 ccagggtcca gatcgataag tcattcccca ttacgccact ccacaaataa atcctttgga
     7861 atcggccgcg gcttgagcgt accggaatcg agacaggcaa cgcgaacgct cgcgtcgatg
     7921 agctgcacgt cgccgcgcgc gatcgtctgc tcgaatagga ttgtggcacc gctcaggcgc
     7981 tcgagttccg cgctgacccg aatttcgtca ttgagcacgg caggacgcag gaagtccacg
     8041 ctgacgttga cgactacgaa ctgtagttgc cgctcggcgc gcagccgctg ttgatcgata
     8101 ttgagcgcgc gcagccagtc gctgcggcag cgttccatga atttcagata attcgcgtaa
     8161 taaacgaccc cgccggcgtc ggtatcctcc caataaacgc ggcattcgat cgagaatctg
     8221 cgcaccggca tctattgtgc atcccggaac aacggcaggt tcaggtcgcg ctcgagcgcc
     8281 ggcgcaggca gcccgaaatg cagataagcg ctgcgtgtgg ccacgcgtcc gcgcgcggtg
     8341 cgcatcagaa atccctgttg aatcaagaat ggctcgatca catcttcgat cgtgccgcgc
     8401 tcctcgctca tcgcggcggc caagctgtcg accccgaccg gaccgccgtc gaatttctcg
     8461 ataaccgtca acagcaattt ccggtccagc atgtcgaatc ccaaaggatc cacttcgagc
     8521 atatcgagcg ccgcgatcgc aacctgcgca ctgatgattc cggccgcctt gacctcggcg
     8581 aaatcgcgta cccggcgtag cagccgattg gcgatgcgcg gcgtgccgcg cgatctttcg
     8641 gcgatgcgca cggcgccgtc ggcttgcagc tgcagtttca agattgcagc gctgcgggtc
     8701 acgatgctgg tcagatcctg cgtcgagtaa aactcgagcc gctgcacgat gccgaaccgg
     8761 tcgcgcagtg gggaggtcag gagtcccgcg cgggtggtcg cgcccaccaa ggtgaacggc
     8821 ggcagatcca gcttgatcga cctcgcgccc ggaccttcgc cgatcatcag gtcgagttgc
     8881 gaatcctcca tcgccggata cagaacctcc tcgaccacgg ggctcaggcg atggatttca
     8941 tcgacgaaca atacgtcgcg tggttccaga ttggtgagca gcgcggccaa gtctcccgga
     9001 cgctccagca ccggaccgga agtctgccga agattgacct tcagttcgtt ggcgatgatg
     9061 ttcgcgagcg tagtcttgcc aagccccggt ggtccgaaaa tcaacacatg atcgagcgcc
     9121 tcgccgcgat tgcgggctgc ctggatgaag atatccatct gggtcttgac gctcgactgt
     9181 cccacgtagt cggccagact cttcggccgc accgcccgat cgagcacttc gtcttcgcgc
     9241 ttgaccggac ctgcaatgat tcgatcctgc tcgatgccca tgctgctgct tacgccttga
     9301 ctgccgactt gagcgcgcga cgtatgattt cggtggtcgc aagatcaggc tcatcgacgg
     9361 tctttagaag ccggactact tccggtggtt tgtagccgag tgcaatcagc gcactgaagg
     9421 cctcgctttg cgcagttgcc gcagggcccg cgcccggcag cgagtcgccc ggcgcgggcg
     9481 tcgagaactt cttcacgcta tcgcgcatct cgatgatcac gcg
//
LOCUS       DASWZT010000500         6435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_13926, whole genome shotgun sequence.
ACCESSION   DASWZT010000500 DASWZT010000000
VERSION     DASWZT010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6435
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13926"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2750
                     /locus_tag="VGE92_14330"
     CDS             <1..2750
                     /locus_tag="VGE92_14330"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein ndvB"
                     /protein_id="HEY1315053.1"
                     /translation="RAKRLAADLIVLNDGPPASAPDLQPALESAIKTAQSGRDTDASR
                     RGAVFALRADTMPPAHRQLLLTAARAVFVAARGTLSEQLARLAEPQPEPRLRRQYPAE
                     ANQEAGTRPTPALEIFNGLGGFAAEAREYVVVLDAGQWTPTPWINVIANAQFGFLAST
                     DGSGSTWSTNAQQNQITPWCNDPVSDMPAEVIYLRDEASGALWSATPLPIREPAASYV
                     VRHGFGYSRYEHSAHGITLELLQFVPLEDSLKISRLKIVNNSARARQLSVTHFVDWVL
                     GNQRSKTAPFIVTEIEATSGALLARNPWSVDFQSRIAFMDMCGRQQTYTADRAEFIGR
                     HGSLAEPKALLGAAPLANRVGGGLDPCGAMQTKISLAPGESTELRLLLGEEASSAAVG
                     ALLNRYRTSDLDAALKEVTDYWDVTLGAIQVKTPDRSMDILANGWLLYQTLVCRVWAR
                     TAFYQSSGAYGYRDQLQDVMALMVSRPKIAREHILRAAGRQFEAGDVQHWWLPTTGQG
                     VKTRVSDDRIWLAFVTAHYLEVTEDLAVLDEQVPYLAGGPLPPDSHEIFSAPGSSSSG
                     SLFEHCVLALDSSLDIGSHGLPLFGTGDWNDGMNRVGAGGRGESVWLAWFLYTSLMQF
                     APIAERQGAREAAARWRKHAFALQQAIEREAWDGDWYRRGYFDDGTPLGSVASDECRI
                     DAIAQSWAVISGAAEPGRAARAMSAVNSQLVSRSDGLVKLFTPPFDRTPHDPGYIKAY
                     PPGIRENGGQYTHAAMWTTWAFALLGDGDRATELFSILNPINHASTRAGIHRYKVEPY
                     VVCADVYSSAQHIGRGGWTWYTGSAGWMYRTAIEGILGMHVRGATLVIDPCIPRAWPG
                     FEITYKFGASRYRIAVENPHGVNRGVRRATLDGQELPGTQCAINLVDDGTYHYGVVTL
                     G"
     gene            complement(2775..3110)
                     /locus_tag="VGE92_14335"
     CDS             complement(2775..3110)
                     /locus_tag="VGE92_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315054.1"
                     /translation="MADRDEAARAAILARLAASREELREILDPPRKEPHGADGAGAGH
                     TPGAFPRSRTMQMLLSGRGLGTLGAAAAGLLVARPALALRLLRLLPVSAIGKMILARA
                     LTAVRSKHA"
     gene            complement(3116..3493)
                     /locus_tag="VGE92_14340"
     CDS             complement(3116..3493)
                     /locus_tag="VGE92_14340"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY1315055.1"
                     /translation="MLWSLPKAAPALLRHLAAYVDLASLDLERSRREIAAEFLASAIV
                     ALCALFVVFMACLAVVAYTWDTPHRVSAIIWMGAGFLAAALLALAYRSNAVRAKSEFL
                     ASVRQAWREDQVLLERILSSDED"
     gene            complement(3509..3889)
                     /locus_tag="VGE92_14345"
     CDS             complement(3509..3889)
                     /locus_tag="VGE92_14345"
                     /inference="COORDINATES: protein motif:HMM:NF017742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HEY1315056.1"
                     /translation="METTFDGTSDPARASGRRLKNDLSEGVNDIRAAATGEIKSLIAD
                     VEDLMARIADLKDADVVRVRSKVQRAVDATKQSLADGADVIRQHAQDVASTADDYVRD
                     RPWQAVGIAALVGAVVGILATRRS"
     gene            4021..5442
                     /locus_tag="VGE92_14350"
     CDS             4021..5442
                     /locus_tag="VGE92_14350"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEY1315057.1"
                     /translation="MTIFEEEGRLGRWYGALPPLILIGFLIGLFLLAAAGQSRLGAAD
                     ERMHDAQLRQQALSEFIALMRDAEAGERGYLLTGEKTYLQPYTDAVPKVAAALDRIYR
                     AYGGQDNRQIEQLRVLTGTRLGELEDTLAVFNKRSPAPALNVVRTNVGKHTMDDISQI
                     VGVMRLADARELAAASAQWRTDFRVSRWLSAVGVILNIALVLLATRFVYGEMRRRARQ
                     AASLREQTQELERQVGLRTQELTALSTHLQGVSEQEKSALSRELHDELGGLLVAARMD
                     LSWLQQRLPTSDPNIEQRFKRIHDSLSAGVDLKRRVVEELRPTLLDNMGLYTALRWQF
                     KETCRRAGLACTELIPESETKFDPDAAIGVFRIAQEALTNILKHAEAKSAHLAVGVDG
                     ANFVLRVTDDGKGIPQNRLLTSTSHGLVSMRHRIAALGGSCDVQSSPGGGTVVVASIP
                     VRRMLRVDLEPKALAAVGEGIRS"
     gene            complement(5465..6289)
                     /locus_tag="VGE92_14355"
     CDS             complement(5465..6289)
                     /locus_tag="VGE92_14355"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY1315058.1"
                     /translation="MNLRLITGMGLALALGACASAPQPNAALESAHAAVTAAEMDPNV
                     AKYAALDLEAAKRDLTIADDAALHHQDAAIAQPAYMAAQNARLAQMHAAAKADDARVA
                     AGQAERDQIMLAARNREVRNATSQRDEATAQNARLQAEVDQLKATPTPRGLVLTLGDV
                     LFDTGRSELNSGSGRKLDQLAQFLGEHKDRRVEIDGFTDSVGTDGYNEELSRRRADAV
                     KSALLTRGIDPSRISTQGYGKAFPVASNSDSGGRQLNRRVEVVIGGDDGTAIAPRG"
BASE COUNT         1132 a         2072 c         2054 g         1177 t
ORIGIN      
        1 ggcgggcgaa gcgcctggcc gccgatctta tcgtgctcaa tgacgggccg cccgcctcgg
       61 cccctgatct gcagccggcg ctggaatcgg ccatcaagac ggcacaatcc ggccgcgaca
      121 cggacgccag ccggcgcggt gcggtatttg cgctgcgcgc cgatacgatg ccgcccgctc
      181 accgccaatt gctgctgacc gccgcccgcg cggtgttcgt cgccgcgcgc ggcacgttga
      241 gcgaacagct ggcgcggctc gccgaaccgc agcccgagcc gcggctgcgc cgtcaatacc
      301 cggccgaagc gaaccaggaa gccggcacgc gcccgacacc ggcgctcgaa atattcaacg
      361 gtctgggcgg ttttgccgcg gaagcgcgcg agtacgtcgt ggtcctcgat gccggccaat
      421 ggacgcctac gccctggatc aacgtgatcg cgaacgcgca attcgggttt ctcgcgtcga
      481 cggacggcag cggcagcacg tggtcgacga atgcgcagca aaaccaaatc acgccgtggt
      541 gcaacgatcc cgtcagcgac atgccggcgg aggttatcta cttgcgtgat gaggcgagcg
      601 gggcgctatg gagcgcgacg ccgctgccca tacgcgagcc cgcggcgtcc tatgtcgtgc
      661 gccacggctt cggttacagc cgttacgaac acagcgccca cggcattact ctcgagctcc
      721 tgcagttcgt gccgctcgag gactcgctga aaatttcgcg cctcaagatc gtcaacaatt
      781 ccgcccgcgc ccggcagctg tcggtcacgc acttcgtgga ctgggtgctc gggaatcaac
      841 gcagcaagac ggcaccgttc atcgtcaccg agatcgaggc gacgagcggc gccctgttgg
      901 cgcgcaatcc ctggagcgtg gactttcaat cgcgaattgc gttcatggac atgtgcgggc
      961 gccagcaaac atatacggcg gaccgcgccg agttcatcgg tcgtcacgga tcgctcgcgg
     1021 agcccaaggc attgctcggc gctgcgcctc tcgcgaaccg agtcggcggc ggactcgatc
     1081 cttgcggcgc gatgcaaacc aaaatcagct tggctcccgg tgaatcgacc gaacttcggc
     1141 tgttattggg cgaagaggca tcctcggctg cggtcggcgc gctgctcaat cgctaccgga
     1201 catcggatct cgatgcagcg ctcaaggaag tgacggatta ttgggacgta accctcggcg
     1261 ccatccaggt gaagacgccc gatcgctcca tggatattct ggccaacggc tggttgctgt
     1321 atcaaacgct ggtctgccgg gtgtgggcga gaaccgcatt ttatcaatcg agcggcgcct
     1381 atggttaccg cgaccagttg caggatgtca tggcgctgat ggtgtcgcgg ccgaagatcg
     1441 cgcgcgagca tattttgcgc gccgcgggcc gccaattcga agcgggcgat gtgcagcatt
     1501 ggtggctgcc gacaaccgga cagggggtga agacccgcgt ctccgacgac cgcatttggt
     1561 tggcattcgt gaccgcccat tacctggaag tcaccgagga cttagccgtg ctcgacgagc
     1621 aggtgccgta cctcgcgggt ggcccgttgc cgccggatag ccacgaaatc ttttcggcgc
     1681 ccggctcgag cagcagcggt tccttgttcg aacactgtgt actcgcactc gattcgagtc
     1741 tcgacatcgg ctcgcacggt ctgccgctat tcggcacggg cgattggaac gacggcatga
     1801 atcgagtcgg tgcgggggga cgcggcgaaa gcgtatggct cgcgtggttc ttgtacacct
     1861 cattgatgca gttcgcgccg attgccgaac ggcagggcgc ccgcgaggcc gccgcacgct
     1921 ggcgcaagca cgccttcgcg ctgcagcaag ccatcgagcg cgaagcgtgg gacggcgact
     1981 ggtatcggcg cggttacttc gatgacggca cgccgcttgg atcggtcgcc agcgatgaat
     2041 gccggatcga tgccatcgct caatcgtggg cggtcatctc cggtgccgcg gaaccggggc
     2101 gagccgcgcg cgccatgtcg gccgtcaact cgcagttggt gagccgcagc gacggcctcg
     2161 tgaaattgtt cacaccgcct ttcgaccgca cgccgcacga cccggggtat atcaaagcct
     2221 atccacccgg catccgcgaa aacggcggcc agtacacgca cgcggcgatg tggacgactt
     2281 gggcgtttgc tctgctgggc gacggcgatc gtgccaccga actattctcg attctcaatc
     2341 ccatcaatca tgcgagcacg cgcgccggaa tacaccgcta caaggtggag ccgtatgtcg
     2401 tgtgtgccga tgtgtattcc tccgcacagc atatcggccg cggcggctgg acttggtaca
     2461 ccgggtctgc gggttggatg taccgcacgg cgatcgaggg aattttggga atgcacgtgc
     2521 gcggcgctac gttggtaatc gacccctgca ttccgcgagc ctggccgggc tttgagatca
     2581 catacaaatt cggtgcgtcg cgttaccgaa tcgccgtgga gaatccgcac ggcgtcaacc
     2641 ggggggtcag gcgcgccacc ctcgacggtc aggagctgcc gggcacgcag tgtgcaatca
     2701 atttagtgga tgacggcacc taccattacg gggtcgtcac gctgggctag gtcggcgctc
     2761 gcttgcgcac gcgtctacgc gtgcttggag cgcacggccg tcagcgcgcg cgccagaatc
     2821 atcttgccga tcgcgctcac cggcagcaat cgcagcaggc gcagcgccag cgccgggcgt
     2881 gccaccagca aaccggccgc cgccgcgccc aatgttccga ggccgcggcc gctcaacagc
     2941 atctgcatgg tgcggctgcg gggaaatgcc ccgggggtgt ggcctgcgcc cgcgccgtca
     3001 gcaccatgcg gctctttgcg cggcggatcg aggatctcgc gcaattcctc acgcgaggcg
     3061 gcgagccttg ccagtattgc cgcgcgtgct gcttcgtccc ggtccgccat acgattcagt
     3121 cctcgtccga tgacagaatg cgctcgagca acacttggtc ctcgcgccac gcctggcgca
     3181 cggaggccag gaactcggat ttggcgcgca ctgcgttcga ccggtaggcg agcgccaaca
     3241 gcgcggcggc caagaatccc gcgcccatcc aaatgatggc actcacgcga tgcggtgtgt
     3301 cccaggtgta tgccacgacc gcgagacacg ccatgaagac cacgaacagc gcacacagcg
     3361 ccacgattgc cgaggcgagg aattcagccg caatctcgcg ccgcgagcgc tccagatcca
     3421 ggctcgcaag atcgacatag gccgccagat ggcgcagcaa cgccggcgcc gccttcggca
     3481 atgaccacag cattcgcacc gtcgtgcctt aagaacggcg ggtcgctaaa atccccacca
     3541 cggcgccgac caaagcagcg atgccgactg cctgccacgg gcgatcgcgc acatagtcgt
     3601 cagccgtgct tgccacgtct tgcgcatgtt gccgtatgac gtcggcccca tccgccaggc
     3661 tttgcttcgt cgcatcaacg gcacgctgaa ccttgctgcg tacccgcacg acatctgcat
     3721 ctttgagatc cgcgatccgc gccatcaggt cttcaacgtc cgcaatcagg ctcttgatct
     3781 cgccggtcgc ggctgccctg atgtcgttga cgccttcgct caaatcgttc ttgaggcgcc
     3841 ggccgctggc gcgcgccggg tccgaggttc cgtcgaatgt ggtttccatt tgttatctcc
     3901 tgtagaaccg agcgaacact accacagcta cccacagcca ttgccggggt ggctgccacc
     3961 cttcctgtag gaatcggccc acatgttaaa ctgttttgct taagtgcgga cacgttgaca
     4021 atgacaatct tcgaggagga gggcaggctc ggtcgatggt acggcgcact gccgccgctc
     4081 atcttgatcg gcttcctgat cgggctattt ttgctcgcag cagccggaca gtcgcggctc
     4141 ggtgcggccg atgaacggat gcacgacgcg cagctgcgcc agcaagcgtt gagcgagttc
     4201 atcgcgctga tgcgcgatgc cgaggccggc gagcgcggct atttgttgac cggcgagaaa
     4261 acctatctgc agccgtacac tgacgcggtg ccgaaagtcg ccgcagcgct cgaccgcatc
     4321 taccgggcct acggcggcca agacaaccga cagatcgaac agttgcgagt tctgaccggc
     4381 acccgactcg gcgagctcga ggacacgttg gcggtcttca ataaacgatc gcccgctcct
     4441 gcgctcaatg tcgtgcgcac caatgtcggc aagcacacga tggatgacat atcgcagatc
     4501 gtcggcgtca tgcgcctggc ggatgcgcgt gaactcgccg ccgcctcggc ccagtggagg
     4561 acggattttc gcgtgtcgcg ttggctgtcg gccgtcgggg tcatactcaa tatcgcgctg
     4621 gtcttgctgg cgactcgttt cgtgtacggg gaaatgcgac ggcgcgcgcg ccaggccgcg
     4681 agcctgcgcg aacaaacgca ggaactggag cggcaggtgg gcctgcggac gcaggagctg
     4741 accgcgctgt cgactcacct ccaaggagtc tcggaacaag agaaatccgc gctgtcgcgc
     4801 gaattgcacg acgagctggg cggcttattg gtcgcggccc gcatggacct gtcctggctg
     4861 cagcagcgcc ttcccaccag cgaccccaac atcgaacagc gattcaaacg catccacgat
     4921 tctttgagcg cgggggtcga tctgaagcgg cgcgtcgtcg aggagctgcg gccgacgctg
     4981 ctcgacaaca tgggcttgta cactgcgctg cgctggcaat tcaaggaaac gtgccgccgc
     5041 gccggcctgg cgtgtaccga actgattcca gagtccgaga ccaaattcga tcccgatgct
     5101 gcgatcggag tcttccgcat cgcgcaggaa gcgctgacca atattctcaa gcacgcggag
     5161 gcaaaatcgg cgcatcttgc ggtcggcgtc gatggcgcca attttgtatt gcgggtgacc
     5221 gatgacggca agggcatccc gcaaaacagg ctgctgacga gcacatcgca cggtttggtg
     5281 tcgatgcgcc atcgaattgc ggcgctcggc ggaagttgcg atgtgcagag tagtccgggc
     5341 ggcgggaccg tcgtcgtggc atcaattcct gtgcgccgaa tgctgcgggt cgacctggag
     5401 ccgaaagccc tcgccgctgt cggcgagggc attcgatcct gactcgaagt ggcctagagg
     5461 tggcctaacc gcgcggcgcg atggccgtgc cgtcatcacc gccgattacg acctcgacgc
     5521 ggcggttcag ctggcggccg ccggagtcgg agttgctggc gaccgggaaa gcctttccgt
     5581 agccttgggt gctgatcctc gacggatcga tgccgcgcgt caacaaagcc gacttgaccg
     5641 cgtccgcgcg ccgccgtgac aactcttcgt tgtacccgtc ggtgcccaca ctgtcggtaa
     5701 agccgtcaat ttccacgcgg cggtccttgt gttcgcccaa gaactgcgcc agttggtcga
     5761 gtttacggcc ggaaccggaa ttcaattccg atcgaccagt atcgaacaac acatcgccca
     5821 aggtcagcac cagtccacga ggcgtggggg tcgccttgag ctggtcgacc tcggcttgca
     5881 gtcgcgcgtt ctgcgccgtc gcttcatcgc gctggctggt ggcattgcgc acttcgcggt
     5941 tgcgggcggc gagcatgatc tggtcacgct ccgcttgccc cgcggcaact cgggcatcgt
     6001 ccgccttcgc ggccgcatgc atttgcgcga ggcgtgcgtt ctgcgcggcc atgtaggccg
     6061 gctgcgcgat cgccgcgtcc tggtgatgca gcgccgcgtc gtcggcgatc gtcaggtcgc
     6121 gtttggcggc ttccaaatcc aacgccgcgt atttcgccac gttcggatcc atttcggccg
     6181 cggtcacggc cgcatgcgcg ctttcgagcg cggcgttcgg ttgcggagca cttgcgcagg
     6241 cacccagcgc cagcgccaat cccattccag taattagtcg taagttcata gagcacctta
     6301 ttgagtggat gtgctgttgc gcatcgattc gctgcgcagc gcctgcatgt tggtatcgaa
     6361 ttccgtggca gccttgtgcg agcgctgttc ctgagcggtt gcagcggcca gccgcgcatc
     6421 tatatcggct tgttc
//
LOCUS       DASWZT010000501        10680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_37921, whole genome shotgun sequence.
ACCESSION   DASWZT010000501 DASWZT010000000
VERSION     DASWZT010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10680
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37921"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VGE92_14360"
     CDS             complement(<1..625)
                     /locus_tag="VGE92_14360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1315059.1"
                     /translation="MSTAERFDICVIGGGLAGLSVAAGLAAARSVVLVEREPHWAYHS
                     TGRSAALFAESYGNTAVRALTRASRDFYLSGDQAAQFTRPRGALFVAAATQQDELEAL
                     HATLSAAASRVEILGPQAALRLCPMLKPELLAAALLDADARDIDVAAVVDHYARQFRA
                     RGGRPYREAEVTRLELVDDAWLIRAGAHEILAPVVVNAAGAWADEVAR"
     gene            714..1865
                     /locus_tag="VGE92_14365"
     CDS             714..1865
                     /locus_tag="VGE92_14365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY1315060.1"
                     /translation="MPRRSVAIPSIKIAVAAAIAVSSASCAQRAPPAAAIVDVTTVTV
                     TPHPVTFSEDYVAQTEAVNAVEIRPRVGGMLEKRVPVEGEPVKTGQLLFVIDREPYIA
                     ALAQAQATLAQSQAASLQSQRDLGRAKSLSEIDAVSQQELDAAVAKNQANLASIDAAK
                     AAVRTAELNLGYTTILSPIDGVMGRAQLRQGGMVTANNTLLTTIYQIDRMYVNFSVSE
                     RRLLSLQRQLGGTMDQKSRTTPPFRIFLADGSEYPQKPRLDFIDPAVDSRTGTLALRL
                     EVDNPHQLLHAGEFARVQLAAQQDPNGILVPQRAVQDLQGKNYVWIVDAAGRAQQRDV
                     QLGPRIGPDWQVQQGLKAGDVVIVDGLQHLKPAAPVKATALAVPAPSEA"
     gene            1862..5005
                     /locus_tag="VGE92_14370"
     CDS             1862..5005
                     /locus_tag="VGE92_14370"
                     /inference="COORDINATES: protein motif:HMM:NF000282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEY1315061.1"
                     /translation="MIDYFIDHPIFSTVLATIITIAGAVSLSGLPVARFPQITPPTIV
                     VSTSFPGADAGTIEQSIAAPIEQQVNGVPHMIYMDSKSANDGSYSLTVSFDVGTAQDI
                     AAVDVQNQVAIAQRQLPAQVLQQGITVAKRQPQILLTVAIGSDDPRYDYLFLSNFATL
                     HVYDALARVRGVGQVLVFGARDYGMRIWLDPAKMARLGVTTDDVTGIINEQNVVAPAG
                     TIGAEPAPAGQQMQYVAAVKGRLSTPEEYAAIVVRTGPDGAVVHLQDIARVELAATDY
                     SRSTKLNGVPTAIIGIYQLPDANALDVAHGVRQAMDALVPTFPKGIHYSIPFDTTLFV
                     SESVHEVVVTLAGAGLLVLLVVFIFLESWRATLIPMVAVPVSLIGAFSAFVALGFSLN
                     TLTLFALVLAIGLVVDDAIVVVEAVTEIMDTRKLAAREATKAAMKEVAGPVVAIALVL
                     ISVFVPVSFLGGLTGQFYRQFALTLAVSVTISLIVALTLTPALCALLLKPTDQSHFGG
                     LLGRAFGAFDRTFKRLTVRYTDSVRKRIRRALQSMLVFLVLSLAAFWLVYSRPTGFMP
                     DEDQGYLMGLVTLPLGASLQRTEAVADAFGRQMRAQPEVQDTFAITGLNILTGTNSPY
                     AATVFAELKPWSKRDDAQHGAPALAARLNRLGAAVKEANVLVFNPPPIPGIGTAGGFE
                     FVLEDRSGGDIQKFAGVLGDFLAQANKRPELARVFTQFNTRTPQIEFALDRERAKTLG
                     VSISSIFASLQTFLGGTYINDFNLFGRTYKVTAQAQAEARAVPDAVNSLYVRTAQGDM
                     VPLSTLVSIKPKLGPEFIERFDVFRAVTIIGSPAPGYSSGQATRAMEELAQDLPSGYG
                     FDWTGTTYQEKISSGQSGAIFGMAIVFVFLVLAALYESWAVPFAVLLCIPFAVLGAYG
                     GLAMRSMQNDIYAQIGLVMLIGLAAKNAILIVEFAKLEYERGKPLVEAAIAGARLRLR
                     PILMTSFAFIFGSLPLAIATGAGASARRVLGTAVVFGMSVATLVGIFLIPVFYVLMQR
                     LAERLGRLWKPAAAAPP"
     gene            4975..6423
                     /locus_tag="VGE92_14375"
     CDS             4975..6423
                     /locus_tag="VGE92_14375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY1315062.1"
                     /translation="METGSGSAAMRTRVTPAVLAAALGALLGGGCALGPDYRRPATSA
                     PAAYRFERDATADSVDDAGWWRVYQDPVLQSLIRDALADNFDVRIAAARVDQARAALG
                     SAHLQQLPQAAINAAAERQRTSIYELPPGIPAVSNIFSLEGNLSYEVDFWGKYRRATE
                     AARAELLQSTYAKQDVTAALVAGVATAYFTLQALDEQLAITQRTVGTRQKFVDLTQAQ
                     HDRGTVSELDVATAQAQLAVAKANVPDLLRRIGQTEDQLSVLLGHNPERIVRSADRAL
                     PPTPAPGLPSSLLERRPDLREAEQNLVSANAQVGVAKANLFPNITLTALDGGVSTALS
                     SLFSGPARTWSLAGGLVQPLLNPQSNLYQLELANAQKRQALLQYQKSVQTAFQEVSDA
                     LIARQQDADVQAAQEAQVDAQRRANTIALARYRVGYASYFNVIDADRDLFTAELSLSE
                     ARLSTLLSVVQLYRALGGGWQAEPASSGAHSP"
     gene            complement(6420..6734)
                     /locus_tag="VGE92_14380"
     CDS             complement(6420..6734)
                     /locus_tag="VGE92_14380"
                     /inference="COORDINATES: protein motif:HMM:NF042692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6587 family protein"
                     /protein_id="HEY1315063.1"
                     /translation="MNSVVDNSLVGLALLLSAGYAVLTLGPRGLRRGLLTALRWIAAR
                     TPAFLGLRRSAVRLAERLAAAASGKAAGACGGCDDCGSEPKSGKSTDGEVTVPVAKIG
                     RR"
     gene            complement(6747..8627)
                     /locus_tag="VGE92_14385"
     CDS             complement(6747..8627)
                     /locus_tag="VGE92_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005407441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HEY1315064.1"
                     /translation="MPSAPSPLIALVGNPNCGKTALFNILTGSRQKVANYAGVTVERK
                     EGSLLTPSGRRVRILDLPGAYSLDPLTPDEQVTADVLLGRRAGESQPDFVVCVTDATN
                     LRQNLRLVLSLKRLGLPMVVALNMSDIARRKGIAIDAERLAAELGVPVVETIGVKTSG
                     VRALVRVLDHLNVPVRGPHSDASARPVWRTPNAADIAHDQSEVRRILGLVGGDRLDGV
                     TVSDRVDAVVLHPVLGPLILALILFLVFQAVFAWAQVPMDAIKSGVAGFGDWLGAALP
                     ASLLKNLLINGVLAGVGSVLVFLPQIIILFFFILLLEDSGYLPRAAFLLDRVMGSVGL
                     SGRAFIPLLSSFACAIPGIMATRTIQNPRDRLATIMIAPLMTCSARLPVYALIIGAFI
                     PHRSLWGGLELQGVVLFVLYVIGVAGAMAVAFVLKRSGASSGFQALMLELPAYHWPNL
                     RNLGIGLWQRVEIFMSRVGTIILALMVILWALSSFPAPPPGALGPPIQYSIAGHIGAW
                     LAVIFAPIGFNWQISIALVPGLAAREVAVGALGTVYALSATGNDVSGALMPLIAQSWS
                     LPTALSLLAWYVFAPQCLSTLATVKRETNSWRYPIIMAGYMFALAYLGSFITYRIALA
                     LS"
     gene            complement(8641..8940)
                     /locus_tag="VGE92_14390"
     CDS             complement(8641..8940)
                     /locus_tag="VGE92_14390"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HEY1315065.1"
                     /translation="MTSSPPSVAVSDAGIRLTELGIGKSARVVAVTAADASAPVDLGR
                     RLAELGFLPGEAVRIVAKGLLARAPIAVRIGTGTFALRLFEAACIRVCPEQPQPA"
     gene            9065..9418
                     /locus_tag="VGE92_14395"
     CDS             9065..9418
                     /locus_tag="VGE92_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005695575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEY1315066.1"
                     /translation="MPTLFEKIIAGELPATVLFRDARVIAFQDIRPAAPTHILIVPHK
                     PIPTVNDIADEDESLIGHMFIVARDLARQLKIADSGYRLIVNCNADGGQEVYHLHMHL
                     LGGRPLGPMISRPSK"
     gene            9429..10529
                     /gene="pip"
                     /locus_tag="VGE92_14400"
     CDS             9429..10529
                     /gene="pip"
                     /locus_tag="VGE92_14400"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEY1315067.1"
                     /translation="MKKKKGVAVKRTAPRPAAKSTTAKSTASKRTTPKRAAQAKTARQ
                     SSQRDSTRRALYPPLEPFRHGYLRVSDVHEIYYEECGNPAGKPAVFLHGGPGAGSDKR
                     ARQFFDPQHYRIVVFDQRGCGRSRPSASLVENTTWHLVADIERLRKHLGIERWLVFGG
                     SWGSTLALAYSEAHPERVSEIVLRGIFLLRYAEIRWFYQHGASEIYPDAWELYRDAIP
                     LDERDDFLSAYHQRLVGSDQRAALAAARAWSIWEASASFLYSNGDNIKKWGEDQFALA
                     VARIECHYFVNRGFLRSEGQLLDDVPRIRHIPATIVQGRYDIVCPATSAWDLHRAWPE
                     ADLRIVQDAGHSAFEPGNAHELVLATDRYRSR"
BASE COUNT         1938 a         3570 c         3348 g         1824 t
ORIGIN      
        1 cgcgcgccac ctcatccgcc catgcgccgg cggcgttgac gacgaccggc gcaagaatct
       61 catgggcccc ggcgcggatg agccacgcat cgtccacgag ttcgagccgg gtcacttcgg
      121 cctcgcgata cggccggccg ccgcgcgcgc ggaactggcg cgcgtagtgg tcgacgacgg
      181 cggccacatc gatgtctcga gcatccgcat cgagcagcgc cgccgcgagc agctcgggct
      241 tgagcatcgg gcacagccgc agcgctgctt gcggccccaa aatttcaacg cgggaagcgg
      301 cggcagagag cgttgcgtgc agcgcctcga gctcgtcttg ctgggtggcg gccgcgacga
      361 acagcgcgcc gcgcggccgc gtaaattgcg ctgcctggtc gccgctcaaa tagaaatcgc
      421 ggctggcgcg cgtcagcgca cggaccgccg tgttgccata agactctgcg aacagcgcgg
      481 ccgagcggcc cgtcgagtga taagcccaat gcggctcgcg ttccaccagc acgacgctgc
      541 gcgcggcggc gaggcccgcc gcgacggaca gacccgccaa accgccgccg atgacgcaga
      601 tgtcgaatcg ttcagccgta ctcatcccct gtgcggcgct ccgttgaggt cgcgcgccca
      661 tcttatgcta ggctaaattc ctcgtgtcga cctgatttga gcacaaaaga accatgcctc
      721 gccgctccgt cgcgatcccg agcatcaaga ttgccgtcgc cgcagcgatt gcggtgtcga
      781 gcgcctcctg cgcacagcgc gcgcctccgg cggcggcgat tgtcgatgtc accaccgtca
      841 cggtcacgcc gcacccggtg acattttccg aggactacgt tgcgcagacc gaagccgtga
      901 acgcggtcga gatccggccg cgggtcggcg gcatgctgga gaagcgggtg ccggtcgagg
      961 gcgagcccgt caaaacaggt cagctgctgt tcgtgatcga tcgcgaaccc tatattgccg
     1021 ctttggccca ggcccaggcg accctggcgc agagccaagc cgcctcgctg caatcgcagc
     1081 gcgacttagg ccgcgccaag tcgctctccg agatcgatgc cgtgagccag caggaactcg
     1141 acgcggcggt ggcgaagaac caagccaacc tggcgtcgat cgacgccgcc aaggctgccg
     1201 tcaggactgc ggagctcaac ttgggctata ccaccatctt gagcccgatc gacggggtca
     1261 tggggcgcgc gcaattgcgc cagggcggca tggtcaccgc caataacacg ctgctgacga
     1321 cgatctacca aatcgaccgc atgtacgtca atttcagcgt cagcgagcgg cgcctgctca
     1381 gcctgcagcg ccagctcggc ggcactatgg atcagaaatc gcggacgaca ccgccgtttc
     1441 gcattttttt ggcggatggt tccgagtatc cgcagaaacc gcggctcgac ttcatcgatc
     1501 ccgcggtcga ttcgcgcacc ggtaccctcg ccctgcgtct ggaggtcgac aacccgcatc
     1561 agctgttgca cgcgggtgaa ttcgcgcgcg tacagctcgc ggcacagcag gatccgaacg
     1621 ggatcctggt gccgcagcgc gccgtgcagg acctacaagg caagaactac gtctggatcg
     1681 tcgacgccgc cggccgcgcg caacagcgcg atgtgcaatt gggcccgcgc atcggccccg
     1741 actggcaggt gcagcaggga ttgaaggccg gagatgtcgt gatcgtcgat gggttgcagc
     1801 acttgaagcc ggccgcgccg gtgaaggcga ctgcattggc ggttccggct ccgagcgagg
     1861 catgatcgac tacttcatcg atcatccgat cttctcgacg gtcctcgcga ccatcatcac
     1921 gatcgccggg gccgtgtcgc tctcgggtct gccggtcgcc cgtttcccgc agatcactcc
     1981 gcccaccatc gtggtttcga cgagttttcc gggcgccgat gccggcacga tcgagcagtc
     2041 gatcgccgcg ccgatcgaac aacaggtcaa cggcgtgccg cacatgatct acatggactc
     2101 gaagagcgcc aatgacggca gctacagcct caccgtgtcc ttcgatgtcg gcaccgcgca
     2161 ggacattgcg gcggtcgacg tgcagaacca ggtcgcgatc gcgcagcgcc agttgccggc
     2221 ccaggtgctg cagcagggca tcaccgtcgc caagcggcaa ccgcagatcc tgcttaccgt
     2281 ggccatcgga tccgacgatc cgcgctacga ctatttgttc ctgtccaatt tcgcgacgct
     2341 gcacgtgtac gacgcgctgg cgcgggtgcg cggagtcggc caggtactgg tattcggcgc
     2401 gcgcgactac ggcatgcgca tatggctcga cccggccaag atggcgcgcc tcggcgtgac
     2461 caccgacgat gtcacgggca tcatcaatga acagaatgtg gtcgcgccgg ccggcacgat
     2521 tggcgccgag ccggcgcccg cgggccagca gatgcagtac gtcgccgcgg tgaaaggccg
     2581 cctatcgaca cccgaggagt acgcggcgat cgtggtgcgc acgggtcccg acggcgcggt
     2641 cgtgcatctt caagatatcg cacgggtgga attggccgcc accgattatt cgcgctccac
     2701 caagttgaat ggcgtgccca ccgcgatcat cggcatctat caattgccgg acgctaacgc
     2761 cctcgatgtg gcgcacggcg tgcgtcaggc catggacgcg ttggtcccga cttttcccaa
     2821 gggcatccac tactccattc ccttcgatac cacgctgttc gtatccgagt ccgtgcacga
     2881 agtcgtcgtg accctggccg gagcgggcct gttggtgctg ctggtcgtgt tcattttctt
     2941 ggaaagctgg cgcgcgacgc tgatcccgat ggtcgcggtg ccggtgtcgc tgatcggagc
     3001 cttcagcgcg ttcgtggcgc tcggtttctc gctcaacacc ctgaccctgt ttgccttggt
     3061 gctcgccatt ggactggtcg tggatgatgc catcgtggtg gtcgaagcgg tcacggagat
     3121 catggacacc cgcaaactcg ccgcccgcga ggcaaccaag gcggccatga aagaggttgc
     3181 gggtccggtg gtcgcgatcg cgctggtgct gatttcggta ttcgtgccgg tgtcattcct
     3241 gggcggcctg accggccagt tctaccggca attcgcgctc accctcgcgg tgtcggtgac
     3301 aatctcgctc atcgtcgcgc tcacgctgac accggcgctt tgcgccttgc tgctcaaacc
     3361 caccgatcaa tcgcattttg gcggcctgct cggccgcgct ttcggcgcat tcgaccgtac
     3421 gttcaaacgc ttgacggtgc gctacacgga ctcggtgcgc aagcgcatcc gccgcgcgct
     3481 gcagtcgatg ctggtgttcc tcgtcttgag cctggcggct ttctggctgg tctacagccg
     3541 acccacgggc ttcatgcccg atgaggacca aggctacctc atgggactcg tgaccttgcc
     3601 gctcggcgcc tcgctacagc gcacggaggc ggtggcggac gccttcggca ggcaaatgcg
     3661 cgcacagccg gaagtccaag atacctttgc catcaccgga ctcaatatcc tgaccgggac
     3721 gaattcgccg tatgccgcca ccgtgttcgc ggaattgaag ccctggtcga agcgcgacga
     3781 tgcgcagcat ggcgcgccgg cgctcgccgc gcggctcaat cggctggggg ccgccgtcaa
     3841 ggaagcgaac gttctggtgt tcaatccgcc gcccatcccg gggatcggga ccgcaggcgg
     3901 cttcgagttc gttctcgagg accgaagcgg cggcgacatc caaaaattcg ccggcgtgct
     3961 cggcgatttc ctggcccaag ccaacaagcg cccggagctc gcgcgcgtgt tcacccagtt
     4021 caacacgcgc acgccgcaaa tcgagttcgc gctcgaccgc gagcgcgcca agacgctggg
     4081 cgtgtcgatc tcgagcatct ttgcgagtct gcagaccttc ctcggcggca cctacatcaa
     4141 cgacttcaac ctgttcggcc gcacctacaa agtgaccgcg caggcgcagg ccgaggcgcg
     4201 cgccgtgccc gacgcggtca acagtctcta cgtgcgcacc gcgcagggcg acatggtgcc
     4261 gctgtcgacg ctggtctcga tcaagcccaa gctcggcccg gaattcatcg agcgcttcga
     4321 cgtgttccgc gccgtcacga tcatcggctc gcccgccccc ggttacagct cgggtcaagc
     4381 cacccgcgca atggaggaac tcgcgcagga tttgccaagc ggctacggct ttgactggac
     4441 cggcaccacc taccaggaga agatctcgag cggccagagc ggcgccattt tcggcatggc
     4501 catcgtcttc gtgttcctgg tgttggctgc attgtacgaa agttgggccg tgcccttcgc
     4561 ggtgctgctg tgcattcctt tcgccgtgtt gggtgcctac ggcgggctcg ccatgcgcag
     4621 catgcaaaac gatatctatg cgcaaatcgg cctcgtcatg ctgatcggtc tcgcggccaa
     4681 gaacgccatc ctgatcgtcg agttcgccaa actcgagtac gagcgcggca aacccttggt
     4741 ggaggcagcc atcgcgggtg cgcgcttgcg cttgcgcccc attctcatga cctccttcgc
     4801 tttcatcttc ggttccttgc cgctcgcgat tgccacaggg gccggcgcct cggcgcggcg
     4861 cgtgctcggc acggccgtgg tattcggcat gagcgtcgcc accctcgtcg gcattttcct
     4921 gattccggtc ttctatgtgc tcatgcagcg gctcgccgaa cggctgggac gcctgtggaa
     4981 accggcagcg gcagcgccgc catgaggacg cgggtaacgc ccgcggtgct cgccgcggcc
     5041 cttggggcgc tcctcggcgg cggctgtgca ctcggtccgg actatcggcg ccccgccacc
     5101 agcgcgccgg ccgcgtatcg cttcgagcgc gatgcgacgg cggattcagt ggacgatgcc
     5161 ggctggtggc gcgtgtatca ggacccggta ctgcagtctc tgattcgcga tgcgctcgcc
     5221 gacaatttcg acgtgcggat cgcggcggcg cgcgttgatc aggcacgcgc cgcactgggg
     5281 tcggcccatt tgcagcagtt gccccaggca gcgatcaacg ccgccgccga gcggcagcgc
     5341 acctcgatct atgaattgcc gcccggcatt ccggcggtta gcaatatatt ctcgctcgaa
     5401 gggaacctct cttacgaggt cgatttctgg ggcaagtatc gccgcgccac cgaagcggca
     5461 cgggctgagt tgctgcaatc cacctacgcc aaacaggacg tgacggcggc gttggtcgcc
     5521 ggcgtcgcca ccgcgtattt cacgctgcag gcgctcgacg aacaactcgc catcacgcag
     5581 cgcacggtcg gcacgcggca gaagttcgtc gacttgaccc aggcccagca cgatcgcggt
     5641 accgtgtccg aattggatgt ggcgactgcg caggcgcagc tggcggtcgc caaggccaac
     5701 gtgccggact tactgcgccg gattggtcaa acggaagacc aattgagcgt gcttctcgga
     5761 cacaatcccg agaggatcgt gcgcagcgcc gatcgggcgc tgccgccgac cccggcgcca
     5821 gggctgccct cgagcttgct cgagcgccgt ccggacttgc gtgaggcaga gcagaatctg
     5881 gtgtcggcga acgcgcaggt tggcgtcgcc aaggcgaatt tgtttccgaa catcacgctc
     5941 accgcactcg acggcggcgt cagcaccgcc ctgtcgtcgc tgttctcagg tcccgcgcgc
     6001 acctggtcgc tggcgggcgg cctcgtccag ccgctgctca atccgcagtc caatctctat
     6061 caattggaac ttgccaatgc gcaaaagcgc caggcactgc tgcagtacca aaagagcgtg
     6121 cagaccgcat ttcaagaagt ctcggacgcc ttgattgcac ggcagcagga tgccgacgtg
     6181 caagctgcgc aggaggccca ggtcgatgcg caacgacgcg ccaataccat tgcgctggcg
     6241 cgctaccgcg tcggttatgc ctcctatttc aatgtcatcg acgccgaccg cgatctcttc
     6301 accgcggaat tgtcgctctc cgaagcgcgc ttgagtacgc tgttgtcggt cgtgcaactt
     6361 taccgcgccc tcggcggcgg ttggcaggcc gagcccgcct cgagcggcgc gcactcgccc
     6421 tagcggcgcc cgatcttggc caccggaacc gtgacctcgc cgtcagtcga tttcccggat
     6481 ttgggttcac tgccgcaatc atcgcagccg ccgcaggcgc cggcggcctt gccggacgcc
     6541 gcggcggcaa gccgttcggc cagccgaacg gcgctgcgcc gcaagcccag gaatgccggt
     6601 gtacgcgcgg caatccagcg caatgcagtc agcaatccgc ggcgcagccc gcgcggaccg
     6661 agcgtgagca cggcgtatcc ggcgctcaac agaagcgcga ggcccaccag agaattgtcg
     6721 acaacgctgt tcaccaaagg ggtccttcag ctgagcgcca gcgcaatgcg ataggtgatg
     6781 aacgagccga gataggccag cgcaaacata tagcccgcca tgataatcgg gtagcgccaa
     6841 gaattcgttt cgcgcttcac cgtcgcgagg gtcgacaggc actgcggcgc gaatacatac
     6901 cacgcgagca gcgacagcgc cgtcggcaga ctccatgact gcgcgatcag cggcatcaat
     6961 gcaccgctaa cgtcattgcc ggtcgccgac agtgcgtaca ccgtgccgag cgcgccgacc
     7021 gcgacttcgc gtgccgcgag gcccggcacg agcgcgatcg atatctgcca attgaaaccg
     7081 atgggcgcga aaatcaccgc cagccacgcg ccgatgtggc cggcaatgct gtattggatg
     7141 ggcggaccca gcgcgccggg cggcggcgcc gggaatgaac tcaacgccca caggatcacc
     7201 atcaatgcca ggatgatggt accgacgcgg ctcatgaata tttccacgcg ctgccacagg
     7261 ccaatgccga gattgcgcaa attgggccaa tggtaggccg gcaactcgag catcagcgct
     7321 tgaaagcccg aggaggcgcc gctgcgcttg agaacgaagg ccaccgccat ggcgcccgcc
     7381 acgccgatga cgtacagcac gaacagcacc acaccctgta actcgaggcc accccacaga
     7441 ctgcgatgcg ggatgaaggc gccgatgatc agtgcgtaca cgggcagccg ggcggaacac
     7501 gtcatcagcg gtgcgatcat gatcgtcgcc aaccgatcgc ggggattttg aatggtgcgg
     7561 gttgccatga tcccgggaat tgcgcacgcg aaactcgaca gcaacgggat gaaggcgcgg
     7621 cccgacagcc cgacgctgcc catcacccgg tcgaggagaa atgcggcgcg cggcagatag
     7681 cccgaatctt ccagcagcag gatgaaaaag aacaggatga tgatctgcgg caagaacacc
     7741 agcacgctgc ccaccccggc gagcacgccg ttgatcaaca ggtttttcaa taggctggcg
     7801 ggcagtgccg ccccgagcca atcgccgaag ccggcgaccc cggatttgat ggcgtccatc
     7861 ggcacctggg cccaggcaaa caccgcctgg aaaaccagga acaagatgag cgcgagaatc
     7921 agcggcccga gcaccggatg cagcaccacg gcgtcgaccc ggtcgctgac cgtcacgccg
     7981 tcgagtcgat cgccgcccac gagacccaag attcggcgca cttcggattg atcgtgcgcg
     8041 atatcggccg cgttcggcgt tcgccacacg gggcgggcgg aggcgtccga gtgcggcccg
     8101 cgcaccggaa cattcaaatg gtcgagcacg cgcacgagcg cccggacgcc gcttgttttg
     8161 actccgatgg tctcgaccac cggcacgccc aattcggcgg cgagcctttc ggcatcgatc
     8221 gcaatgccct tgcggcgcgc gatgtcgctc atgttgagcg cgaccaccat gggcaagccc
     8281 agccgtttca ggctcaacac caggcgcagg ttttggcgca ggttggtggc atcggtgacg
     8341 cacaccacga agtcaggctg agactcgccc gcgcggcggc cgagcagaac atcggcggtc
     8401 acctgttcgt cgggcgtcag gggatcgagg ctgtaggccc ccggcaagtc caaaatgcgc
     8461 acgcgccgcc ccgatggggt gagcagcgac ccttccttgc gctccacggt gacgccggcg
     8521 taattggcga ccttttgacg gctgccggtg agaatgttga atagcgcggt tttaccgcag
     8581 ttggggtttc caaccagggc gatcagagga ctcggagcgc taggcacagc ctccatggag
     8641 tcacgcgggt tgcggctgct ccggacaaac gcggatacag gcggcttcga acaatcgcaa
     8701 cgcgaacgtg ccggtaccga tccgcacggc aatcggcgct cttgccagta gtcccttggc
     8761 cacgatgcgc accgcttcac cgggtaagaa acccaattca gccaagcgcc gccccaaatc
     8821 caccggtgcc gatgcatcgg cggccgtgac cgcgaccact cgggcacttt tcccgatccc
     8881 taactccgtc aaccggatgc cggcatcgga aaccgctacg ctgggaggag aactcgtcat
     8941 ggatgcaatt ctacatgaga atcgttccca ttactaataa ggaacgctct gctgcaccgc
     9001 aaaattcaag cggcgctacc gttcctgcta atcttggagc cccttcagcc tcggaggcat
     9061 tgaaatgcct acgcttttcg agaaaatcat cgccggcgag ctgccggcca ccgtactctt
     9121 tcgggacgcc cgggtgatcg cttttcaaga catccggccc gcagccccga cgcacatatt
     9181 gatcgtcccg cacaagccca tccccaccgt gaatgacatc gccgacgagg atgaatccct
     9241 gatcggacac atgttcatcg tggcgcggga cctcgcgcgt caactcaaga tcgcggactc
     9301 tggctatcgc ttgatcgtca attgcaatgc ggacggcggc caggaggtgt atcacttgca
     9361 catgcacctg ctcggtgggc ggccgctcgg cccgatgatc tctcgcccat caaagtaaag
     9421 gacctccgat gaagaagaaa aagggcgtgg cggtcaaacg caccgcaccg cggcctgccg
     9481 cgaagagcac taccgcgaag agcaccgcct cgaagcgcac cactccgaag cgcgcggcgc
     9541 aggcgaaaac cgcacggcag tcgagtcagc gcgacagtac gcgccgcgcc ttgtatccgc
     9601 cgctcgagcc ctttcgccac ggctacctgc gcgtctcgga tgtgcatgag atctactacg
     9661 aagagtgcgg caacccggcc ggcaagccgg cggtgttcct gcacggcggt ccgggcgcgg
     9721 gatccgataa acgcgcgcgg caattcttcg atccacagca ctaccgcatc gtggtgttcg
     9781 atcaacgcgg ctgtggccgc agccgtccga gcgcgagcct ggtggagaat acgacctggc
     9841 atctggtcgc cgacatcgag cgcttgcgca agcacctcgg catcgagcgc tggctggtgt
     9901 tcggcggatc gtggggcagc accttggcgc tcgcatattc cgaagcgcat cccgaacgcg
     9961 tcagcgaaat cgtgctgcgc ggcatattct tgctgcgcta cgcggagatt cgctggttct
    10021 atcagcatgg cgcctcggaa atctaccccg atgcctggga gctttaccgc gacgcgatcc
    10081 cgctcgacga gcgcgacgac tttttgagcg cctaccacca gcgcttggtc ggcagcgatc
    10141 agcgcgccgc attggcggcg gcccgtgcct ggtcgatatg ggaggcgtcg gcgagctttc
    10201 tctacagcaa cggcgacaac atcaagaaat ggggcgagga ccagttcgcc ctcgccgtgg
    10261 cccgcatcga atgccactac ttcgtgaatc gcggcttctt gcgcagcgaa ggtcagttgc
    10321 tcgatgacgt gccgcgcatc cgccacattc ccgccaccat cgttcaggga cgctacgaca
    10381 tcgtctgccc ggcgaccagc gcgtgggatc tgcaccgcgc gtggccggaa gcggacttgc
    10441 gcatcgtgca ggacgccggg cactcggcat tcgagcccgg caatgcgcac gaactcgtgc
    10501 tcgcgaccga tcggtaccgc agccgctaat cccctttacc cgagaccgac atgcgccact
    10561 ttcacgccac ctgcgtcgcg gctgccctgg cattctgtgc cgcggggcac gccgccggca
    10621 cgaaactgct gcgctttccc aatgtgcggc aggatcggat tgtgttcagc tatgccggag
//
LOCUS       DASWZT010000502         4958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_52345, whole genome shotgun sequence.
ACCESSION   DASWZT010000502 DASWZT010000000
VERSION     DASWZT010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52345"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /gene="cydB"
                     /locus_tag="VGE92_14405"
     CDS             complement(<1..622)
                     /gene="cydB"
                     /locus_tag="VGE92_14405"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEY1315068.1"
                     /translation="MHADLTMVWAGIIIFAVAMYVVMDGFDLGIGILFTRFDVGVERD
                     TSMNAIAPVWDGNETWLILGGGGLMAAFPLAYAVILPALYAPMIAMLLGLVFRGVAFE
                     FRWREPNHRARWDAGFCVGSIVATFAQGVTLGALLQGIKMSGRAYAGGYWDWLTPFSV
                     LTGASLVVGYALLGACWLIWKTSGMVQAEARRFAAVLTPGLLAAIAA"
     gene            complement(622..2013)
                     /locus_tag="VGE92_14410"
     CDS             complement(622..2013)
                     /locus_tag="VGE92_14410"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY1315069.1"
                     /translation="MTATGTALTLARAQFAFTISFHFIFPAFSIGLASYLAVLEALWL
                     KTGKPVYDNLYRYWVKIFAIVFGMGVVSGIVMSYQFGTNWAVFSDKAGPILGPLMAYE
                     VLSAFFLEAGFLGVMLFGINKVGTRLHFLATCMVAAGTLLSATWILAANSWMQTPAGW
                     MLNEAGQFVPQGSWLTIIWNPSFPYRFVHTVIAAFLTTSLAVGAVGAWHLLRDRNNAG
                     ARTMFSMAMWMAALVAPLQILAGDTQGLNTQEYQPAKVMAMEGHFESYAAGAPLVLLG
                     IPDEANRRMTYSVEIPKLSSLILAHDWNAPLAGLDTIPRRDWPPVAIIFWSFRVMVGL
                     GLAILGLGLFSLLARARGKLYEWSLLHRLAVTMGPAGFIAVIAGWVTTEVGRQPFTVY
                     HALRTADSVSPIATPAVTGSLLAFVVVYFATFVSGAIYILKLMAKPPSTHESPPEAAP
                     IHAAGITPAAGLK"
     gene            2133..3860
                     /gene="ggt"
                     /locus_tag="VGE92_14415"
     CDS             2133..3860
                     /gene="ggt"
                     /locus_tag="VGE92_14415"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY1315070.1"
                     /translation="MTTLTTLTVAAGARAASPPALEASTGMVVTAQHFASEAGAAVLR
                     QGGNAVDAAVAVGYALAVTHPCCGNLGGGGFMMIHLADGRNTFINFREKAPLAARADM
                     FLDPHGDPMSDKSVNGYLAVGVPGTVLGLETARQEYGTLARPVLMAAAIRLADDGFVV
                     TRGDVDELEVGSPDFHAQPNVAAVFLHDGAAYKPGERLVQRNLAATLRAISDGGAAAF
                     YHGPIAGAVVAASRANGGLLTEQDFAAYGVTESAPLSCRYRGYTILSAPPPSSGGVAL
                     CEMLQVLERYPLHALGFHSSESVHLMTEAMRSAYRDRNTLLGDPAFVDNPVERLLSAR
                     HTDEIRARIRPQRATPSSALGSAAADREHATTTHYSVVDSAGNAVAVTYTINDDFGAK
                     VIAGESGFFLNDEMDDFTAKPGAGNLFGLVQGKANAIAPGKRPLSSMTPTIVLKDGKP
                     VLVVGSPGGARIITTVLEVIVNVIDHGMTLQEAVDAPRIHHQWLPDTLAGEPFALSAD
                     TVKALKRMGYRVVPLKPWGTGNAAEVIGIAPADAAQAEELGFPRPGIFYGASDSRAPA
                     GSAAAPELR"
     gene            complement(3800..>4958)
                     /locus_tag="VGE92_14420"
     CDS             complement(3800..>4958)
                     /locus_tag="VGE92_14420"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEY1315071.1"
                     /translation="VYFQGSRMTAEQALRSAGLAPIQPFAADDNALTSSNAYATGQAA
                     LLVHDARAALEWSDLIYAMDLDGMNSSITPLSRVVQRDRPEKWLNWHAARILDMIKGS
                     YLFDKDPKRIIQDPESLRASSIRQGSTWEAWGALRDAVIFQANSSDHNPAVNVGLSPE
                     DSWELATPQLLRFFVKGGPHSHGQHGYIVSNANWDPYPMANQVENFVIALANMDIAVM
                     LRIERLRNPFFTGVALREILPGVNGGFFDYAPVDLQQEVQSLTTPIAPFGSAIVGTVE
                     DLQAQTRLKMQRARQAVETTFDLLGFDLIEASLWMDARKTQDPARAFGAAPTAAWNAF
                     RQRVPLRQDAAPAQSISLVASQFLKTTPASTYYRSSGAAAEPAGARESLAP"
BASE COUNT          996 a         1598 c         1497 g          867 t
ORIGIN      
        1 cagccgcgat tgccgccaat agccccggag tcaagacagc ggcgaagcgg cgagcctcgg
       61 cctgcaccat gccggacgtt ttccaaatca accagcacgc ccccaacaag gcatagccca
      121 ccacgaggct cgccccggtc aacacactga acggcgtaag ccagtcccaa tatccgccgg
      181 cgtaggcccg cccgctcatt ttgattccct gcaatagcgc gccgagcgtc acgccttgcg
      241 cgaacgttgc aacgatggag cccacgcaaa atcccgcgtc ccaccgcgcg cggtggttcg
      301 gctcgcgcca acggaactcg aacgcaaccc cgcgaaacac gagtccgagc agcatggcga
      361 tcatcggcgc gtacagcgcc ggcaagatga cggcgtaggc cagcggaaag gccgccatca
      421 ggccccctcc tcccaaaatc agccaggttt cattgccgtc ccagacgggc gcgatggcgt
      481 tcatcgacgt atcgcgctcc actccgacat cgaatcgtgt gaacaggatg ccaatgccta
      541 aatcgaagcc atccatgacg acgtacatcg cgaccgcgaa gatgatgatg cctgcccaaa
      601 ccatcgtaag atctgcgtgc atcacttcaa cccggcggcc ggtgtgattc cggccgcgtg
      661 aattggcgcc gcctcgggcg gcgactcgtg ggtgcttggc ggttttgcca tcagcttgag
      721 aatgtagatc gcgcccgata caaaagtcgc aaagtacacc acgacgaagg ccagcaagga
      781 gccggtgacg gccggagtcg caattggtga caccgagtcg gcggttcgca gtgcgtggta
      841 aaccgtgaac ggctggcgcc cgacctctgt ggtcacccac ccggcgatca cggcaatgaa
      901 tcctgccgga cccatagtca cggcaagacg atgcagcaaa ctccactcgt acaatttgcc
      961 gcgcgcccgc gccagcaaac tgaaaagacc gagacctaga atcgcaagcc ccaaaccgac
     1021 cattacgcga aacgaccaga aaatgatggc gaccggcggc cagtcgcgcc gcgggatggt
     1081 atcgagtccg gcgagcggtg cattccaatc gtgcgcgagt atcaatgagg aaagcttggg
     1141 gatctcgacg ctataggtca tgcggcgatt cgcctcgtcc ggaattccga ggagcaccag
     1201 aggagccccc gcggcgtaag actcgaagtg tccctccatt gccatgactt tggccggttg
     1261 atattcctgc gtattcaaac cttgggtatc gccggcgaga atttgcagcg gagcgaccaa
     1321 ggcggccatc cacatggcca tcgagaacat ggtgcgcgca ccggcgttgt ttcggtcgcg
     1381 cagcagatgc catgcgccga ccgcacccac ggccaacgag gtggtgagga acgcggctat
     1441 cacggtgtgg acgaagcgat acggaaaact cggattccaa atgatggtga gccaagaacc
     1501 ctgcggcacg aattggcccg cctcattcag catccagccg gccggcgtct gcatccaact
     1561 gttggctgca agtatccatg ttgccgacaa caaagtcccg gccgcgacca tgcaagtcgc
     1621 gagaaaatgc agccgcgtgc caactttgtt gattccgaag agcatcacgc cgagaaatcc
     1681 ggcttcgaga aaaaacgccg acagtacttc gtaggccatg agcggaccga ggatggggcc
     1741 ggccttgtcc gagaacacgg cccagttcgt tccgaactga taggacatga cgatgcccga
     1801 gacgacgccc atgccgaaga cgatcgcgaa tatcttgacc cagtatcgat acaagttgtc
     1861 gtagaccggt ttgccggtct tgagccaaag agcctcgagc acggccagat agcttgccag
     1921 tccgatcgag aacgcgggga agatgaaatg aaaagaaatc gtaaacgcga actgcgcgcg
     1981 cgccaacgta agtgccgtac ctgttgccgt cattgatggt tcctcgagcc gcgctgcgct
     2041 actctagccc gtgctcgcca cctcccgtcg gttagtttac cccaggaagg ctcctcggaa
     2101 tgcatgttcg cctcgaaata gcatcgctcg ctttgaccac tttgaccact ttgaccgtag
     2161 ccgctggtgc gcgcgctgcc tctcccccgg cattggaagc gagcaccggc atggtggtca
     2221 cggcacagca tttcgcctcc gaggccggcg cagcggttct gcgccaaggc ggaaacgccg
     2281 tcgatgccgc ggttgccgtg ggttacgcac ttgcagtgac gcatccatgc tgcggcaatc
     2341 tcggcggcgg cggcttcatg atgatccatt tggcggacgg cagaaatacc ttcatcaatt
     2401 ttcgcgagaa ggcgccgctc gctgcgcgcg cggacatgtt tctcgacccg cacggcgatc
     2461 ccatgagcga taagagcgtc aacggttatc tcgctgtcgg cgtgcccggc acggtgttgg
     2521 gactcgaaac ggctcggcaa gaatacggca ccttggcacg gccggtgctg atggccgctg
     2581 cgattcgctt ggcagacgac gggttcgtcg ttacgcgcgg cgacgtcgat gaactcgagg
     2641 tcggcagccc cgactttcat gcacagccca acgtcgcagc ggtgtttctg cacgacgggg
     2701 cggcctacaa acccggcgag cgcctggtgc aacggaatct ggctgcgacc ttgcgcgcca
     2761 tcagcgatgg cggcgccgca gcgttttatc atggaccgat tgccggcgcc gttgtcgcgg
     2821 cctcgcgcgc caacggcggc cttctcaccg aacaggactt tgccgcatac ggcgtcaccg
     2881 aatctgcacc cctctcctgc cgctaccgcg gctacacgat tctgtcggcg ccgccgccga
     2941 gttccggcgg agtcgcgctc tgcgaaatgc tgcaagtcct cgaacgttat cccctgcacg
     3001 ctctcgggtt tcactccagc gagtcggtgc atctcatgac cgaggccatg cgctccgcct
     3061 atcgcgatcg caacacgctg ctcggcgatc ccgcgttcgt cgacaatccg gtggagcgcc
     3121 tgctgtcggc gcgacatacc gacgagatcc gcgcacgcat taggccgcaa cgcgcaacac
     3181 cctcgtcggc gctcggcagt gcggcggccg accgcgagca cgccaccacc acgcactact
     3241 cggtcgtcga ctcggccggc aacgcggtcg cggtcaccta cacgatcaat gacgactttg
     3301 gcgccaaagt tatcgcaggc gagagtggat ttttcttgaa cgacgaaatg gacgacttca
     3361 cggcaaagcc cggcgcgggg aacctcttcg gactggtgca aggcaaggca aacgcgattg
     3421 cgccgggcaa gcgccccttg agctccatga cccccacgat cgtgttgaag gacggcaagc
     3481 cggtgctggt ggtgggttcg cccggtggcg cgcgcatcat taccacggta ttggaggtca
     3541 tcgtcaatgt aatcgaccac ggcatgacgc tgcaggaagc ggtcgatgcg ccgcgcatcc
     3601 atcatcaatg gttgccggac accctggccg gcgaaccctt cgccctctcc gccgatacgg
     3661 tcaaggcatt gaaacgcatg ggctatcgcg tggtgccgct caaaccctgg ggaaccggca
     3721 atgcggccga agtaatcggc atcgcacccg ccgacgcggc gcaggccgag gaacttggct
     3781 ttccgcgccc cggcattttc tacggagcca gcgactcgcg agcgccggcc ggttcggccg
     3841 ccgcacccga gctgcggtaa taagtcgacg ccggcgtagt cttcaagaat tgcgacgcca
     3901 ccagcgaaat cgattgcgcc ggagccgcat cttggcgcag cggcacacgc tggcgaaacg
     3961 cgttccacgc cgcggtcggc gccgcaccga aggcgcgcgc gggatcctgg gtcttgcgcg
     4021 cgtccatcca taagctggcc tcgatcaggt cgaaccccag cagatcgaac gtcgtctcga
     4081 ccgcttgccg cgcgcgttgc atcttgaggc gcgtctgcgc ctgcaggtct tcgaccgtcc
     4141 ctacgatggc gctgccgaaa ggcgcaattg gagtggttag gctctggact tcctgctgca
     4201 aatccacggg cgcgtagtcg aagaacccgc cgttcacgcc gggtagtatc tctcgcagcg
     4261 cgacgcccgt gaagaaggga ttgcgcaagc gctcgatgcg cagcatcacg gcgatgtcca
     4321 tattggcgag cgcgatcacg aaattctcga cttgattggc catcgggtaa ggatcccaat
     4381 tggcgttcga cacgatgtag ccgtgttggc cgtggctgtg cgggccaccc ttgacgaaga
     4441 agcgcagcaa ctgcggcgtc gccagttccc aagaatcctc cggcgacaga cccacattga
     4501 cggccgggtt gtggtcggag gaatttgctt gaaaaatcac cgcgtcgcgc agcgcgcccc
     4561 acgcctccca ggtggaacct tggcgaatgg acgaggcccg cagactttcc ggatcttgaa
     4621 tgatgcgctt cgggtccttg tcgaacagat agctgccctt gatcatgtcg agtatgcggg
     4681 cggcgtgcca attcaaccat ttctcgggcc gatcgcgctg cacgacgcgg cttagcggcg
     4741 tgatgctgct gttcatgccg tcgaggtcca tggcataaat caagtccgac cactcgagcg
     4801 ccgctcgggc atcgtgcacc aggagcgccg cctggccggt ggcgtacgca ttgctgctgg
     4861 tcaatgcatt gtcatcggcg gcaaacggtt gaatcggggc gagaccggcg ctgcgcagcg
     4921 cctgttcggc cgtcatccgc gacccttgaa agtacacc
//
LOCUS       DASWZT010000503         6659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_76476, whole genome shotgun sequence.
ACCESSION   DASWZT010000503 DASWZT010000000
VERSION     DASWZT010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6659
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76476"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1995
                     /gene="edd"
                     /locus_tag="VGE92_14425"
     CDS             187..1995
                     /gene="edd"
                     /locus_tag="VGE92_14425"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008038612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEY1315072.1"
                     /translation="MHIRVREVTERIRERSRSSRAEYLARMEAARQQGSSRAGLSCTN
                     LAHGFAASDATDKQALKLLRWPNVAIVSAYNDMLSAHQPLERYPALIKRAARDAGAVA
                     QFAGGVPAMCDGVTQGQPGMELSLFSRDVIAMATAVALSHNMFDSALCLGICDKIVPG
                     MLMGALAFGHLPVIFVPGGPMPSGVPNKEKARVRQLYAEGKAGRDELLESEAGSYHSA
                     GTCTFYGTANSNQMLMEVMGLHLPGSAFVPPNTPLRDALTAAAAQRAARITALGSEYL
                     PLANGVDEAAIVNAIVGLLATGGSTNHTLHLVAIAHCAGISITWDDFSDLSAAVPLLT
                     RIYPNGSADVNRFHVAGGMGFLIRELLQGGFLHADTLTVMGPGLAAYAMEPWLDGGKL
                     AWRAAPATSGDLDVLRPLSDPFSTHGGLRLLTGNLGRAVIKTSAVKHEHHVVEAPALV
                     FDDQQEVIDAFKSGKLERDFVAVVRFQGPRANGMPELHKLTPSLASLQSRGFKVALVT
                     DGRMSGASGSVPAAIHVTPECVNGGPLGKVRDGDVLLVDCHSGELQAKVAEDVWQRRE
                     SPTLDHARNESGCGRELFASLRRAAGGAEAGALSLL"
     gene            2022..2648
                     /gene="eda"
                     /locus_tag="VGE92_14430"
     CDS             2022..2648
                     /gene="eda"
                     /locus_tag="VGE92_14430"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEY1315073.1"
                     /translation="MNIREIVAMAPVIPVLTVTNLDHAVPLARALAGGGLRVLEITLR
                     TPVALAAVEAMRKAVPDAIVGVGTLTRAVDFAAADRVGAQFGVTPGLTPELAAASRGA
                     RFPLLPGVMTPTELITARTAGFNVLKLFPAQQAGGLAMLQALGAPFPDVLFCPTGGIT
                     RATAADYLALPNVVCVGGSWVAPKQMLEAGDWSGIEALARDAAGLRKA"
     gene            2645..4000
                     /locus_tag="VGE92_14435"
     CDS             2645..4000
                     /locus_tag="VGE92_14435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY1315074.1"
                     /translation="MKDLTDGPIARNIVQMALPIAAGMFFQTLYFLIDLYFVSGLGDA
                     AIAGVGAAGNVMFIVFALTQVLGVGTVALISHAVGRKDQADATLVFNQSLVLSASCAL
                     LSLVLGYAFGPAYMRTLGSDVATAAAGTRYLYWFLPSLGLQFALVVMGSALRGTGIVQ
                     PTMIVQVLTVILNALLAPVLIAGWGTGHPMGVAGAGLASSLSVGVGVGLLGLYFHKLE
                     KYVAFDSGAWRPQFATWNRLLRIGLPAGGEFALMFIYMAVIYWIIRRFGADAQAGFGI
                     GQRLTQSMFLPAMAVAFASAPIVGQNFGAKKFERVRETFTLATLIGGCIMVLLTLFCQ
                     WGSAWAIHWFTSDATALAVGGQFLQVISWNFLATGIVFTCSAVFQGLGHTLPAVLSSA
                     SRIVSFVVPALWLASQPHFQLLHLWYVSIGSATLQACVSLWLIRSEFRRRLPAVSASP
                     A"
     gene            complement(4025..4657)
                     /gene="hisIE"
                     /locus_tag="VGE92_14440"
     CDS             complement(4025..4657)
                     /gene="hisIE"
                     /locus_tag="VGE92_14440"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF002747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HEY1315075.1"
                     /translation="MNPKNLDWDKGGGLLPAIVQDARNGAVLMLGYMNRAALAASESS
                     GRVTFWSRSKGRLWTKGESSGNYLQVIAIAADCDGDALLVSAEPAGPACHLGTPTCWG
                     VDPPQSRAQPLAFLGELEQVIAQRIATRPEGSYTSRLLAEGTRRIAQKVGEEGLELAL
                     AGIAQSDQEIIGEAADLLFHALLLLKVKGLSLSQVVAELKTRHVGRATAP"
     gene            complement(4654..5394)
                     /gene="hisF"
                     /locus_tag="VGE92_14445"
     CDS             complement(4654..5394)
                     /gene="hisF"
                     /locus_tag="VGE92_14445"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014998871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEY1315076.1"
                     /translation="MDVRDGQVVKGVQFRNHRIVGDILELAARYRDAGADELVFYDIS
                     ASPEGRSVDHSWVARVARTLDIPFCVAGGIRSVAQAEAVLASGAEKISVNSPALERPE
                     LIDELSRRFGAQCVVVGVDSLSVDQGYRVQQFTGDVGRTRDTSRATIDWVIEAQRRGA
                     GEIVLNCMSSDGVRRGFDIAQLRAVREVCDVPLVASGGAGAVAHFAEVFQAAHVDAAL
                     AASVFHSGEIGIPSLKLELRAAGIEVRA"
     gene            complement(5403..6143)
                     /locus_tag="VGE92_14450"
     CDS             complement(5403..6143)
                     /locus_tag="VGE92_14450"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HEY1315077.1"
                     /translation="MQLIPSIDLKAGACVRLLHGDFAHETRYELTPQALLAKYRDLGA
                     AWLHIVDLDGAKTGSLDNRALISQLAAAQSVKLQVGGGLRNTAAVTQTLDLGVARVAI
                     GSAAVTHVDQVRAWLDHFGPERITLAFDVRLDGGGIPRVTTHGWQRQSQLTLWGALEQ
                     FAGSPLTHVLCTDVSRDGALSGPNIDLYREAAQRHPLIQWQASGGVRDAHDLRALAAA
                     GAAAAVSGKALLEGLIAAEDLQPFLPNA"
     gene            complement(6146..>6659)
                     /gene="hisH"
                     /locus_tag="VGE92_14455"
     CDS             complement(6146..>6659)
                     /gene="hisH"
                     /locus_tag="VGE92_14455"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEY1315078.1"
                     /translation="SVSADAAEIGAATHVILPGVGAAADAMSRLRQSGLDRLIPMLQQ
                     PVLGICLGMQLLYQASEEGDTACLGIIPGRAARIAAARDRPVPHMGWNTLDIRRPCEL
                     LAGLVDGDYAYFVHSYALEMTSATVASTRYGAAFSACVQWRNFYGAQFHPERSAAVGA
                     RLLKNFLAIH"
BASE COUNT         1088 a         2132 c         2202 g         1237 t
ORIGIN      
        1 cctgcccggc tgcgaagcgg cggtacagcg ccggcagcag cttgcgcgtc accagatcgc
       61 cggtgccgcc gaaaagcaac atgtcgaagg tcggaagtac attcaccggc aaagcgtagc
      121 atttgcgtgc gcgttctagt aagtcttcgc gatatggcac aatgaagcat gccgcagacc
      181 cagccgattc atattcgcgt gcgtgaagtc accgagcgga ttcgcgagcg cagtcgatcg
      241 agccgcgccg agtacctggc gcgcatggag gcggcgcgcc agcagggttc gtcgcgcgcg
      301 gggttgtcgt gcaccaatct cgcgcatggc ttcgccgcat cggatgccac ggacaagcag
      361 gcgctcaagc tgttgcgctg gccgaacgtg gcgatcgtgt cggcctacaa cgatatgctg
      421 tccgcacacc aaccgctgga gcgatatccg gccttgatca agcgggcggc ccgtgatgcc
      481 ggcgcggtcg cgcaattcgc gggcggagtg cccgcgatgt gcgatggcgt gacgcagggt
      541 cagcccggca tggagctgtc cttgttcagt cgcgatgtga tcgcgatggc aaccgctgtc
      601 gcgctgtcgc acaacatgtt cgactcggcc ctgtgtctcg gaatctgcga caaaatcgtc
      661 ccgggaatgc ttatgggtgc gcttgccttc gggcatttgc cggtgatatt cgtgccgggc
      721 ggtccgatgc cgtccggcgt tccgaataag gagaaggcgc gcgtgcgaca gctgtacgcg
      781 gagggcaagg ccggccgcga cgagctgctc gagtccgagg cgggcagcta ccacagcgcc
      841 ggtacctgta ccttctacgg cacggccaac agcaaccaga tgttgatgga ggtcatgggg
      901 ctgcacctgc cggggtccgc tttcgtaccg ccgaacacgc cattgcgcga tgcactgacg
      961 gccgcggccg cgcagcgtgc ggcgcgcatc accgccttgg gatccgagta cctgcctctt
     1021 gccaacggcg tcgatgaagc ggcgattgtg aatgccatcg tcggcctgct tgcgaccggc
     1081 ggctcgacca atcacacctt gcacctggtg gccattgcgc actgcgccgg catcagcatc
     1141 acctgggacg atttcagcga tttgtcggcg gcggtgccgc tgttgacgcg catctatccg
     1201 aacggcagcg ccgacgtgaa tcgatttcac gtggccggcg gcatgggatt cttgatccgt
     1261 gagctgctgc agggcggctt tctgcatgcc gacacgctga ccgtaatggg tcccgggctt
     1321 gctgcctacg cgatggaacc ctggctcgat ggcggcaagt tggcctggcg cgcggcgccc
     1381 gcgaccagcg gcgatctcga cgtattgcgg ccgctcagcg atccattcag cactcacggc
     1441 ggcttgcggt tgctcacggg caatctcgga cgggcggtca tcaagacttc ggcggtgaaa
     1501 catgaacatc acgtggtgga agcgccggcg ttggtgttcg acgatcaaca ggaagtcatc
     1561 gatgcattca agagcggcaa gctcgagcgg gatttcgtgg ccgtcgtgcg ctttcaaggg
     1621 ccgcgcgcca acggcatgcc cgagctgcac aagttgacgc cgtcgctcgc gtctttgcag
     1681 agcaggggtt tcaaggtggc gctggtcacc gacggccgca tgtcgggcgc atcgggcagc
     1741 gtgcccgccg ccattcacgt cacccccgaa tgtgtcaacg gcggcccgtt gggcaaggtg
     1801 cgcgacggcg atgtgctgct ggtggattgt cacagcggcg agttgcaggc caaagtcgcc
     1861 gaggacgtgt ggcagcggcg cgagtccccg acgctcgatc acgcacgcaa tgaatccggc
     1921 tgtgggcgcg aattattcgc cagcctgcgc cgcgccgccg gcggtgcaga ggccggtgcg
     1981 ctttccctgt tgtgaacttt cccacatcgt aaggatcgcc cgtgaacatc cgtgaaattg
     2041 tggcaatggc gccggtcata ccggtgctca ccgtgaccaa tctcgaccac gccgtgccgt
     2101 tggcgcgcgc cttggccggc ggtggcttgc gcgtgctcga gatcacgctg cgcacacccg
     2161 tggcgctcgc cgccgtcgag gcgatgcgca aggccgtgcc cgacgccatc gtcggcgtcg
     2221 gcacgctcac gcgtgcggtg gattttgcgg ccgccgatcg cgtgggtgcg cagttcggcg
     2281 tgacgccggg cctgacgccc gagctcgcag cggcctcgcg cggcgcacgc tttccgttgc
     2341 tgccgggggt gatgacgccg accgaactga tcacggcgcg caccgcgggg ttcaacgtct
     2401 tgaaattgtt tccggcacag caggccggcg ggttggccat gttgcaggcg ctcggcgcgc
     2461 cgtttcccga cgtcttgttc tgtccaaccg gtggcatcac gcgggcgacc gccgcggatt
     2521 atttggcgct gccgaacgtc gtttgcgtcg gtggttcgtg ggtggcgccg aaacagatgc
     2581 tggaggccgg cgattggagc ggcatcgaag ccttggcgcg cgatgcggcc ggcttacgca
     2641 aggcatgaaa gatctaaccg acggtccgat cgcgagaaat atcgtgcaga tggcgctgcc
     2701 gatcgcggcc ggcatgttct ttcagacgct ctacttcctc atcgatcttt acttcgtctc
     2761 cggattggga gacgcggcca tcgccggcgt aggagccgcg ggcaacgtca tgttcatcgt
     2821 gtttgcgctc actcaggtgc tcggcgtcgg caccgtggcg ctgatttcgc atgcggtggg
     2881 gcgcaaggac caggcggatg cgaccttggt cttcaatcaa agcctggtgt tgtccgcgag
     2941 ttgcgcgctg ctgagcctgg tactcggcta cgcgttcggc cccgcctata tgcgcacctt
     3001 gggttcggat gtggcgaccg ccgcagccgg aacccgctac ttgtattggt tcctgccgag
     3061 cctcggtttg caattcgcgc tggtggtgat gggttcggcg ctgcgcggca cgggcatcgt
     3121 gcaacccacg atgatcgtcc aggtgctgac ggtgattctg aatgcgctat tggctccggt
     3181 gttgatcgca gggtggggca ccggccatcc gatgggcgtc gccggggcag gccttgcaag
     3241 ttcgctgtcg gtcggcgtcg gtgtcgggtt gctcggcctg tacttccaca agctggaaaa
     3301 atacgtggca ttcgattccg gcgcgtggcg tccgcagttt gccacgtgga atcggctgtt
     3361 gcgcatcgga ttgcccgcgg gcggagagtt tgcgctcatg ttcatctaca tggcggtgat
     3421 ctattggatc atccgccgct tcggcgccga cgcccaggcc ggcttcggca tcggccagcg
     3481 tctgactcaa agcatgtttc tgccggcgat ggccgtcgcg ttcgcgtcgg cgccgattgt
     3541 tggccagaac ttcggcgcga agaaatttga gcgtgtgcgc gagacgttta ccctggccac
     3601 gctgatcggc gggtgcatca tggtgctgct caccttgttt tgccagtggg gctcggcgtg
     3661 ggccatccat tggtttacca gcgacgcgac ggcgctcgcc gtcggtggac agtttctgca
     3721 ggtgatctcc tggaacttcc tggcgacggg aatcgtattt acctgttcgg cggtgttcca
     3781 aggtctcggg catacgctgc cggcagtact cagttcggcg agccgtatcg taagcttcgt
     3841 cgtgccggcg ctgtggctcg cctcacagcc gcattttcaa ttactccacc tctggtatgt
     3901 ctccatcggc agcgcgacgc tgcaagcgtg cgtcagtctc tggctgatcc gcagcgaatt
     3961 tcgacggcgt ctacccgcgg tatcggcgtc gccggcttga ctcgcgcgtg gccctcgccg
     4021 gcgctcaagg tgcagtagcg cgtccgacgt gtcgtgtctt gagttccgcg acgacttgcg
     4081 acagcgacag acccttcacc ttgagcagca gcagggcgtg gaacagaaga tccgccgcct
     4141 cgccgatgat ctcctgatcg gattgcgcga tgcccgccaa cgccagttcc agcccttctt
     4201 cgccgacctt ctgggcgatc cgccgcgtcc cctcggccaa gagtctgctg gtatagctac
     4261 cctcgggacg ggtcgcgatg cgttgtgcaa tgacttgctc gagctcgccg aggaaagcca
     4321 acggctgtgc gcgcgattgc ggcggatcca cgccccaaca cgtgggcgtt cccaggtgac
     4381 aggccgggcc ggccggttcg gccgacacca acagtgcatc gccgtcgcaa tcggccgcta
     4441 tcgcaatcac ctgcagatag tttccggagc tctctccctt ggtccaaagg cgtcctttgc
     4501 tgcggctcca aaaggtgacc cggccgctcg attcgctcgc cgcgagcgcg gctcgattca
     4561 tataacccaa catcagcacc gcgccgttgc gggcgtcttg gacgatcgcc ggcagcaggc
     4621 cgccgccctt gtcccagtcg agatttttcg ggttcatgcg cgaacctcga tgcccgctgc
     4681 gcgcaattca agcttcaagc ttggaattcc aatctcgccg gagtggaaca cgctcgcggc
     4741 cagggcggcg tcgacatgcg ccgcctggaa aacttcggcg aaatgcgcga ccgcgccggc
     4801 cccgccggac gcgaccagcg gcacgtcgca cacttcgcgg accgcacgca gttgcgcgat
     4861 gtcgaaaccg cggcgcactc cgtcactcga catgcagttg agcacgattt caccggcgcc
     4921 gcgccgctgc gcctcgatca cccagtcgat ggtggcgcgc gaggtatcgc gcgtgcgccc
     4981 gacgtcgccg gtgaactgct gcacgcgata gccctgatcg acgctcaggc tgtcaacgcc
     5041 tacgaccacg cactgcgcgc cgaagcgacg gctcaactcg tcgatcaact cgggacgctc
     5101 caacgccggc gagttgaccg agattttctc ggcgcccgac gccagcaccg cttcggcttg
     5161 ggcgacgctg cgaattccgc cggccacgca gaatggaata tcgagggtgc gcgcgactcg
     5221 cgcgacccag gagtgatcga ccgagcggcc ttcggggctc gcagagatgt catagaaaac
     5281 caattcatcg gcgcccgcgt cgcgatagcg ggccgcgagt tccaaaatgt cgccgacgat
     5341 ccgatgattg cggaattgca cgcccttgac cacctgccca tcgcggacat ccaagcaagg
     5401 gattatgcgt ttggcaagaa tggctgcaaa tcctccgccg caatgagtcc ctcgagaagt
     5461 gccttgccgc tgaccgcagc ggcggcaccg gctgcagcca atgcacgcag atcgtgggcg
     5521 tcgcgcacgc cgcccgacgc ctgccattgg atcagggggt ggcgctgcgc ggcctcgcgg
     5581 tacaggtcga tattcggtcc gctcagggcg ccgtcgcggc tcacatcggt acacaggaca
     5641 tgcgtcagcg gcgagcccgc gaattgttcc agcgcgcccc acagcgtgag ctgtgactgg
     5701 cgttgccatc catgggtcgt gacgcgcgga atgccgccgc cgtcgagccg cacatcgaat
     5761 gccagcgtga tgcgctccgg gccgaagtga tcgagccacg cgcgcacctg gtccacatga
     5821 gtgacggcgg cgctgccgat cgccacccgc gcgacaccga gatccagagt ctgagtcacg
     5881 gcagcggtat tgcgcagtcc gccgccgact tgcagcttga ccgattgcgc tgccgccagc
     5941 tgcgagatga gcgcgcgatt gtcgaggctg cccgtcttgg cgccgtcaag gtcgacgata
     6001 tgcagccaag ctgctccgag atcccggtat ttcgcgagca gggcctgcgg cgtcagctcg
     6061 tagcgcgtct cgtgcgcgaa atctccgtgc agcaggcgca cgcaggcgcc ggccttgaga
     6121 tcgatcgacg ggatgagctg catgctcaat ggatggccaa gaaattcttg agcagccgcg
     6181 cgccgaccgc ggcggagcgt tcggggtgaa actgcgcgcc gtagaagttg cgccactgga
     6241 cgcaggcgct gaaggcggcg ccgtagcgcg tgctggcgac cgtggcggac gtcatctcga
     6301 gtgcgtagct gtgcacgaaa taggcgtaat cgccgtcaac gagacctgcc agcagttcgc
     6361 agggccggcg aatgtccaga gtgttccaac ccatgtgcgg caccggacga tctcgcgccg
     6421 ccgcgatacg cgcggcacgg ccgggaatga tcccgaggca cgccgtgtcg ccttcctccg
     6481 aggcttgata caacaattgc atacccaaac agatccccag taccggctgc tgcagcatgg
     6541 ggatgagccg gtccaggccg ctctgacgca gtcgcgacat ggcatccgcg gcggcaccga
     6601 cgccgggcag aatgacatgg gtcgcggcgc cgatttccgc ggcgtcggcc gacaccgac
//
LOCUS       DASWZT010000504        12320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_71746, whole genome shotgun sequence.
ACCESSION   DASWZT010000504 DASWZT010000000
VERSION     DASWZT010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12320
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71746"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="VGE92_14460"
     CDS             <1..1015
                     /locus_tag="VGE92_14460"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY1315079.1"
                     /translation="CYIVTEHVHGARTLAAYCRPDNLLRIDDVVELIYKAARALHYAH
                     SRGVIHRDIKPSNIMLTLESDLRIIDFGIALVADSEISRIEGIAGSPSYMSPEQVQSI
                     ELTNRSDLYSLGAVMYELLTGTRPFRAGNLQKLLHQIVFATPPPIHTLRPEIPEELEN
                     VTAMALLKDPEKRYKTGLDFAAELTRVHQKLRAQYNRIDQQEQFSLLRKLKFFHEFSH
                     GEIWEVLRASSWQDYADAEEIVKEGEMDDRFYIIVQGRVSVQRLARALGYLESGDCFG
                     ETSYVRGAKRTATINAAGPVTVMKVSSTLLEQVSAECQLRFNQVFLRSMIGRLQSAER
                     GVS"
     gene            complement(1012..1836)
                     /gene="folE2"
                     /locus_tag="VGE92_14465"
     CDS             complement(1012..1836)
                     /gene="folE2"
                     /locus_tag="VGE92_14465"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HEY1315080.1"
                     /translation="MNVAVPAKNPKTKPVEDVQGHADTRQIPINKVGIKDIYHPIKVK
                     DRARGEQHTVANFNMYVNLPHNFKGTHMSRFVEILHRHEREISVDSFGKMLVEMTEHL
                     AAGAGHIEMTFPYFVMKMAPVSGVESLMNYQATIFGEHRAGNTEVWLKVVVPTTSLCP
                     CSKKISDYGAHNQRSHITLTAKLVEHMWLEELIDIAEKQASCEVYGILKRPDEKFVTE
                     RAYDNPKFVEDTVRDIAVALNKDKRVRAYVVESENFESIHNHSAYAMIEHDKEAAD"
     gene            complement(2111..2359)
                     /gene="xseB"
                     /locus_tag="VGE92_14470"
     CDS             complement(2111..2359)
                     /gene="xseB"
                     /locus_tag="VGE92_14470"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007894317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEY1315081.1"
                     /translation="MPTPDTKKAAQPDFETAMRDLEELVERLEQGDLPLEESLAAFER
                     GVLLTRACQAALKEAEQKVEILLKKAGEPALEDFTPDG"
     gene            2542..2991
                     /locus_tag="VGE92_14475"
     CDS             2542..2991
                     /locus_tag="VGE92_14475"
                     /inference="COORDINATES: protein motif:HMM:NF033894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HEY1315082.1"
                     /translation="MPNSVKSSAACAALAAIMLVAAGCSPRRIQPMSVTDLMEDRVTL
                     DGVLLKCGQNPAKARTDTDCLNARIAIDRLASRSDPAEEAKRTEEFEHNRERLRMSQD
                     KQRQEEEAKTKVDAYHLPLVPVEPPPAADAPAPSSDANPPVARESNP"
     gene            complement(3003..4796)
                     /locus_tag="VGE92_14480"
     CDS             complement(3003..4796)
                     /locus_tag="VGE92_14480"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEY1315083.1"
                     /translation="MRLIAVVFVCAAHFLLTPTSVQAQGATHDALVSLAQDVTYTSAR
                     LYPLQATALGIPGYDADLETPSEEFRAAYIQRLRQWQQQLQTISAGFDSKTSLVDRDD
                     ATLLAAQLAAAVDSLLVYQTDRKDYSAAANGIVAAIFTQFENLPVVGRDGATPQDVDK
                     AWHDIISRLAKAPAYIVAAQRLATTPGHLYGVVGAEQLGNVSDFFRGALDAAAKAQFR
                     THRAALAQFERALDAALATIAESKAHIDAYAASWPENFAMGRKAYDELLRREQLLPYD
                     SRSVEQMGRDELAHGWAEEAWLTALARRNATPFGAPSGGGMAPSGAPLIDYYRDRIAE
                     LRRFVLQNDVVTIPDWLGSMQVIETPAFLQPVLPGAAMNPPRPFAASTTGYYYITPPT
                     SLEKAAASLDMNEDFDHDRILSTAAHEAMPGHFLQLSIARRHPDFIRKIQNSGVFAEG
                     WAFYGEEMFVKLGLYGGNLDGRLHTARWERVRGVRAIVDPQLASGELTYEQAVEFFAT
                     QTGYTKDAARAAVAGIATSPGYVIAYTVGRLQLENLLGAYLLRMGGRGSLHDFHDRLL
                     SYGTTPFAIVAPELLADLDQPANAVRAAANF"
     gene            4937..5452
                     /locus_tag="VGE92_14485"
     CDS             4937..5452
                     /locus_tag="VGE92_14485"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HEY1315084.1"
                     /translation="MKFRNANFAVLLGLAVLSAAAGQERPPATDPSAVLERLLGEWQM
                     RGDVRGTPVQYRLAGTRVLQGTFVELHMTDVARPAKYEARVFIGYDQQGKRVIAHWMD
                     SFGAAYSIPHGVGAIEGNQIQFEIPYADGPFRDTFVYDRFRDVWTLNIESGDGKGSWR
                     HFACYTITKRP"
     gene            complement(5549..7933)
                     /locus_tag="VGE92_14490"
     CDS             complement(5549..7933)
                     /locus_tag="VGE92_14490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1315085.1"
                     /translation="MHFGIAEIVEVNYRTGRLLPLAVALIGILLTGNARGDCVPLSSP
                     ELRLLDRLTDGDPERAVAQAERRLTLLPLRHDALVEAELYAVIAAARAQQGRSGEAHA
                     AVLAAGHVLDTQPDSSGTQRLRDRLLMNDIESAETESNLRSAMQSITGIIARTPARSI
                     EQLCALAARADLHAEHLELDLAAADGLAAYSQAEDAGLTEPRIQAAASLANTFRRSGL
                     LWEAERMMDEVVQFERSENRVSQLAAAMYVRGQILIEGQRYGEARQTLESSRRLAMEV
                     GDEFGASFTNVALCPALISAGDLDSAQQVCSASDSLFAAAGREDLVTLMLAYRARLDL
                     ARGQPQQALAKLDQVLGPRAAYIMSTEEPKIHLDYARALGALGQFDYAYRELAHSLEL
                     QRSLDLAQRSRAAAVLKVAYDAERRAARSRSITQRLWIAFAAAATLLSILFGCLLWSG
                     HRHKRQLRRQQVVLQTVTSSAPDALLLLDRQRTVRFANRSTFGSGPTPAQGESLDNIV
                     SSSMLGSLKAAVDQVFENRCANTFQTDFIDETGAVRRFELRSVPVIEDNELIGVTLCS
                     MDITEIRRLEREVLDVVTSERLRLSSDMHEGLGQELTGISLLLKSVVDRIERGKPATS
                     EELSEIMAFVSHTIGMTREIANGLSPIRIQRGSFAAALQHLAQESSRRLRLKITVDPD
                     CDDIVVSEEVADHLYRIALEAITNAARHSGCSKIEISFRLANDGLHLSISDDGVGVRP
                     KAVDADGMGLKMMAYRGRLIGGAIRFEARPGGGSQLIVVAPIVNGFSEPLATAG"
     gene            complement(8106..8816)
                     /locus_tag="VGE92_14495"
     CDS             complement(8106..8816)
                     /locus_tag="VGE92_14495"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY1315086.1"
                     /translation="MPSRMHAALLDAACEPYRNAGRFAYHFARGKLRTDPVYRAILEL
                     GLLRGRARVLDLGCGQGLLSAWLRAAERCYDRGSWPRAWPPAPRALSTRGIELMARDV
                     ERARCALGPSSEVSHGDIRDAAFGAVDAVVILDVLHYMAPQAQLDVLQRVRTALPSRG
                     LLLLRVGDAGGGLRFHYGQWSDRLVMLLRGHAIVTQHCRSVAQWRKLLRECDFDSEAK
                     PMSRGTPFANVLLIAHAR"
     gene            complement(8823..9389)
                     /locus_tag="VGE92_14500"
     CDS             complement(8823..9389)
                     /locus_tag="VGE92_14500"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY1315087.1"
                     /translation="MMPKYVVFMTGLASACLPLLSQAADPPPDASSAVQTREQIEQAL
                     HPQQMHTRGFGTRSFTRTDAEPPEPPQQPMSINLNIPFDHNSSAITRKAESQLKQLQL
                     ALTGASLGKDRFEVAGHTDAKGNPDYNKQLSYKRAEAVKQFLIANGVGADRLQTVGFG
                     SEHLLAPDRPDDPRNRRVEIRDLGSAAH"
     gene            complement(9386..10795)
                     /locus_tag="VGE92_14505"
     CDS             complement(9386..10795)
                     /locus_tag="VGE92_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315088.1"
                     /translation="MQTAQSAPPAADKAAQNPEVRAYVDDTCIVADEPYFLPGNKEAN
                     ARFLPLLGLIVGKLAELFVNHEIEASANRIKAGAARKDTRYAVITQMNLYRVDFQSGP
                     ALSINAKLGCMTIVAATFKPEPASCTADYLPKELAPETKDLPQDQWKTSRTDDSIENQ
                     LRRANVCVVGKVKAVYEARFEFSKDGTAYRLKDAGYHIESLLTTADKGAARTTLYTLK
                     VSTPSVTDQQEVLSSAWVNIGTVSAGSHSSGSKGDAAPWLRVPPLSVEARRVYDEKTR
                     QQQETMGEIDALKRAMTRDQRMLASLDQRIAAAGADTAEGLKQERTRIAVQYQSQGAE
                     LDARNAEYLDLPRTPLEIMPVTVEVAVTETESEKKAQLALADIIGKNSDLVGSAVGNS
                     ATGLLSKSVSAADIITEPDAADSASALAAARSRYYDALVEVKSSAPGAASADAQRNLA
                     AMKDRYNEARRSQGLEQIK"
     gene            11054..>12320
                     /locus_tag="VGE92_14510"
     CDS             11054..>12320
                     /locus_tag="VGE92_14510"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEY1315089.1"
                     /translation="MGFRGRVLPPAALLLCSILCHPAAARNIALLVGVGTFQAPGLQG
                     AKLLGVPNDLVSMQKGLTQRWGFQPGDVVVLHDQDATHDRILEQISALQQRSTAGDTV
                     LIYYSGHGTSANDNHNKYDLPYDSGAWVPYDVDVSSPKATQLSLIVGRRDLVPRLSAL
                     DKGGRWVVVVSDSCFSGQVVRAFGETHSTSRYLPLQTRDLGVSQAQPAPAPVAVTPRP
                     TPPPYPYQHVLLLSGASDSESGADISSDEGLRQAPTIDNQYHGAFTDAFLRLMNGQFY
                     PAGSFNYAQGRQAMVDWMEHNFQQHPQLLPTVAEDPLNVAARPFLALQTAATAAPAAG
                     VAAAAATAARSTTLHVKLESVSPALKTQIASLSGVALVDAKPDMTVKQHGEQVDLRGP
                     AGDPIVTKPASDPALIKRISAQAWLNHVLP"
BASE COUNT         2288 a         3788 c         3849 g         2395 t
ORIGIN      
        1 ctgctatatc gttaccgagc acgtccatgg tgcgcgcacg ctggccgcgt attgccgccc
       61 cgacaactta ttgcgcatcg atgacgtggt cgagctgatc tacaaggcgg cgcgcgcgct
      121 gcattacgcg cacagccgcg gcgtcattca tcgcgacatc aaaccgagca atatcatgct
      181 cacgctggaa agcgacctgc gcatcatcga tttcggaatc gccttggtgg cggattcgga
      241 gatctcgcgg atcgaaggca tcgccggcag tccttcgtat atgtcgcccg aacaagtgca
      301 gtcgatagag ctcaccaatc gctccgactt gtactcgctc ggtgcggtga tgtacgagct
      361 gttgaccgga acccggccgt tccgcgcggg caatctgcaa aagctgctgc atcagatcgt
      421 gttcgcgacg ccgcctccca ttcacacctt gcgccccgag atccccgagg agctcgagaa
      481 cgtcacggcc atggcgttgc tcaaggaccc ggaaaagcgc tacaagaccg gcttggattt
      541 cgccgccgaa ttgacgcgtg tgcaccagaa gctgcgcgcg caatacaatc gcatcgatca
      601 gcaggaacag ttcagcctgc tgcgcaaact gaaatttttc cacgaattct cgcacggcga
      661 gatttgggaa gtgttgcgcg ccagcagctg gcaggattac gccgacgctg aagagatcgt
      721 caaggaaggc gaaatggacg atcgctttta catcatcgtg caggggcgcg tcagcgtgca
      781 gcgcttggcg cgggcgctcg gctacctgga gagcggcgat tgcttcggcg agaccagtta
      841 tgtgcgcggc gccaagcgca ccgccaccat caatgccgcc ggaccggtga cggtcatgaa
      901 ggtcagctcg acgctgctcg agcaggtgtc cgccgagtgc cagttgcgct tcaaccaggt
      961 tttcctgcgc agcatgatcg ggcgtctgca gagcgcggag cgcggcgttt cctagtcagc
     1021 ggcttccttg tcgtgctcga tcatcgcgta ggcggaatga ttgtggatcg actcgaaatt
     1081 ctccgactcg accacgtagg cgcgaacccg tttgtccttg ttgagcgcca ccgcgatatc
     1141 gcgcaccgta tcctcgacga acttcggatt gtcgtaggcg cgttccgtca cgaatttctc
     1201 gtcgggccgc ttgaggattc cgtacacctc gcatgaagcc tgcttctcgg cgatatcgat
     1261 cagctcttcg agccacatgt gctcgaccag ttttgcggtc aaggtgatgt gcgagcgctg
     1321 attgtgggcg ccgtaatcgg agattttctt cgagcacggg cacaggctgg tcgtcgggac
     1381 cacgactttg agccaaactt ccgtgttacc cgcgcggtgc tcgccgaaaa tcgtggcctg
     1441 gtaattcatc aggctctcga cgccggatac cggggccatt ttcatgacga agtacggaaa
     1501 agtcatctcg atatggcccg cgcccgcggc cagatgctcg gtcatttcca ccagcatctt
     1561 gccgaacgaa tccaccgata tctcgcgctc gtgccgatgc aaaatctcga cgaagcgcga
     1621 catgtgcgtg cccttgaaat tatgcggcaa gttaacgtac atgttgaagt tcgcgacggt
     1681 gtgctgctcg ccgcgtgcac gatccttcac tttgatcggg tgatagatgt ctttgattcc
     1741 gaccttgttg atcggaattt gccgcgtatc ggcatgaccc tgcacgtcct cgacgggctt
     1801 cgttttagga ttcttggcgg ggactgcaac gttcatagag gcctacatgg tgccaaaatc
     1861 gggaattcca ataatcttag acgattcgcg cgcgcttggc tcacgacccg agccggactc
     1921 cggccggcgg cgggcgatac ggcgcacggt atcccccaat tggggccacg ccaacccagt
     1981 gtgcagtgat ttggcccgcg cggcagccaa tttcgccgct cgcgaccgcc aggcagtcta
     2041 aacgcgacga tcagcaacgg ccgcaggcat tgccgcggct caagactgca tagcgctgcg
     2101 ccgcgtgtgc tcatccgtcg ggagtaaaat cttcgagcgc aggctctccg gccttcttca
     2161 gcaatatctc gactttctgt tccgcttcct tgagtgcggc ctggcaggcg cgtgtcagca
     2221 gcacaccgcg ttcgaaagcg gcgagggact cctcgagcgg caggtcgccc tgttcgaggc
     2281 gttccaccag ctcctcgaga tcgcgcatcg cagtctcaaa gtcgggctgt gcggcttttt
     2341 tggtatccgg ggtaggcata agttaagaac ggtacacagc cgttgcaacc gggtcaaacg
     2401 agggtgcagc gccggcttgg cggccgatcg gggcgggccg aaggaatctt gggatggaac
     2461 attgtcaagc cgtgatatgc gtcacagccg cgggcgcgtt gacgcggctg ggtttgagta
     2521 ccattgcatg tgagaagcca tatgccaaat tcagtcaaat cgagcgccgc ttgcgcggct
     2581 ttagcggcga ttatgctggt cgccgccggc tgcagtccgc ggcgcattca gcccatgtcg
     2641 gtcaccgatc tcatggagga tcgggtgacg ctcgatggcg tgctgctgaa gtgcggtcag
     2701 aatccggcca aggcccgcac cgatacggat tgtctgaacg cgcgaattgc catcgaccgc
     2761 ctcgccagcc gcagcgatcc agccgaagag gcgaagcgca cggaggagtt cgagcacaat
     2821 cgcgaacggc tgcgaatgtc gcaagacaag cagcgccagg aagaagaagc gaagaccaaa
     2881 gtagatgcgt atcacttgcc gctggtgccg gtcgaaccgc cgccggccgc agacgcgccg
     2941 gcgccgtcct ccgacgcgaa cccgccggtc gcccgggaat caaaccccta agaaatacag
     3001 tttcaaaagt tcgccgccgc gcgtaccgca ttcgccggct ggtcgagatc cgccagcaac
     3061 tccggggcga caattgcaaa aggcgtcgtc ccgtacgaca gcaggcgatc gtgaaagtcg
     3121 tgcagtgagc cgcgcccgcc cattctcaac aggtacgcgc cgagcagatt ctcgagttgc
     3181 aagcgcccga ccgtataggc aatgacatac cctgggctcg tggcaatgcc cgcgaccgcg
     3241 gcgcgtgcgg catctttggt gtatccggtc tgggtggcga aaaactcgac ggcttgctcg
     3301 taggtcaatt ctcccgaggc gagctgcgga tcgacaatcg cgcgcacccc gcgcacccgc
     3361 tcccagcgtg cggtatgcaa acggccgtcg agattgccgc catacaggcc gagtttcacg
     3421 aacatctcct cgccgtagaa tgcccagcct tccgcgaata cgccgctgtt ttgaattttg
     3481 cggataaaat ccggatgccg cctggcgatg gatagctgca gaaaatgtcc gggcatggct
     3541 tcatgcgccg cagtggaaag aattcgatcg tgatcaaaat cctcattcat gtccaagctc
     3601 gcggccgctt tctcgagcga tgtcggcgga gtgatgtagt aataaccggt ggtcgaggcc
     3661 gcaaaaggcc gcggtggatt catcgccgcg ccgggcaata ccggctgcag aaaagccggc
     3721 gtttcgatga cctgcatcga gccgagccag tccggaatgg tcacgacatc attctgcagc
     3781 acgaatcgac gcagctcggc aatccgatcg cgatagtagt cgatgagcgg tgcgccgctg
     3841 ggcgccatgc cgccgccgct cggcgccccg aacggcgtcg cgttgcgccg cgccagcgcc
     3901 gtcagccacg cttcttccgc ccacccgtgg gcaagttcat cgcggcccat ttgttcgacg
     3961 gagcgcgaat cgtaaggcaa gagctgttcg cgacgcagca gctcgtcgta ggctttgcgg
     4021 cccatcgcga aattctccgg ccatgatgcc gcgtacgcat cgatgtgagc cttggattcg
     4081 gcgatggtcg ccaaggccgc gtcgagcgca cgctcgaact gtgcgagtgc cgcgcgatgg
     4141 gtgcgaaatt gagccttggc ggcggcgtcg agcgcgccgc gaaaaaagtc agagacgttg
     4201 cccagttgtt cggcgccgac cacgccgtac agatgacccg gcgtcgtcgc caagcgctgc
     4261 gccgccacga tataggccgg agccttcgcg agacggctga tgatgtcatg ccacgccttg
     4321 tcgacatcct gcggcgtcgc gccgtcgcgg ccgacgaccg gcagattctc gaattgcgtg
     4381 aagattgcgg cgacgatacc gtttgccgcg gcgctgtaat ccttgcgatc ggtctggtac
     4441 acaagcaagg agtcgacggc ggcggcgagc tgcgccgcga gcaaggtggc atcatcccga
     4501 tcgaccagcg atgtcttgga gtcgaagccg gccgagatcg tctgcagttg ctgctgccat
     4561 tggcgcaggc gttggatgta cgcggcgcga aattcttcgc tgggagtctc gagatccgcg
     4621 tcgtagccgg gtatgccgag tgccgtcgcc tgcagcgggt agagacgggc cgaggtatac
     4681 gtcacatcct gcgcgaggct caccaaagcg tcatgcgtcg cgccttgcgc ctggactgag
     4741 gtcggcgtca gcagaaagtg ggcggcacaa acgaaaacta cagcaatcaa gcgcattgat
     4801 tttttctccg cgtgaggggt tgcgccggcc gccattcgag cgtacatgtt catggcgaaa
     4861 tattacagcg gctgcacggc aggcgagcct cgaggcgggc ggcaatcggc cgcgccgtga
     4921 tttggagtac gggcccttga aattccgcaa cgccaacttc gctgtgctgc ttggattggc
     4981 cgtcttgtca gccgccgcgg gacaggagcg gccgccggcg acggatccgt cggcggtttt
     5041 agagcggttg ctcggcgagt ggcagatgcg cggggatgtg cgcggtacac cggttcaata
     5101 tcgtctcgca ggcacacggg tgctgcaggg gacgttcgtc gaattgcata tgacggatgt
     5161 ggcacgtcct gcgaaatacg aagctcgggt gttcatcggg tacgaccagc agggtaagcg
     5221 cgttatcgcg cattggatgg acagcttcgg agcggcgtat tcgatcccgc acggtgtcgg
     5281 cgccattgag ggcaatcaga ttcaattcga gattccctac gccgacggac cgtttcggga
     5341 taccttcgtt tacgaccggt ttcgggacgt gtggacgctt aacatcgagt ccggcgatgg
     5401 caagggaagt tggcgccatt tcgcctgtta cacgatcacg aagcggcctt gagcaagggc
     5461 gagcggcgct gttaaggcga aggtccttgc cggctcaatg acttggcccg tatgcttaag
     5521 attgacgttc aagtcgggcc gcggtgcatt accctgcggt tgccagcggt tcgctaaagc
     5581 cattgacgat cggcgcaacg acgatgagct gcgagccgcc tcccggccgc gcttcgaacc
     5641 gaatcgcacc accaatcaat cgccctcgat aggccatcat ttttagaccc atcccgtccg
     5701 cgtcaacagc cttggggcgc acacccaccc cgtcgtccga gatgctcaga tgtaggccgt
     5761 cgttcgcgag tcggaacgaa atttcgatct tggaacagcc gctatgcctg gccgcgttgg
     5821 taatcgcctc aagagcgatt ctgtacaggt ggtcggcgac ctcctcggac acaacgatgt
     5881 cgtcgcagtc gggatctacc gtgatcttga ggcgcaggcg gcggctcgac tcctgggcca
     5941 gatgctggag tgccgcggcg aatgagccgc gctgaatccg aatgggcgac agtccattcg
     6001 cgatctcacg agtcataccg atcgtatggc tcacgaacgc catgatttcg gacaattctt
     6061 cactcgttgc cggcttgccg cgctcgattc tgtccactac gcttttcaac agcaacgaga
     6121 ttcccgtgag ctcctgcccg agcccctcgt gcatgtcgct gctgagacgc agccgttcgg
     6181 aggtcaccac gtcgagcact tctctctcca ggcgccgaat ctccgtgata tccatcgagc
     6241 aaagcgtcac gccgatcaat tcgttgtcct cgatgacggg gacactgcgc agctcaaacc
     6301 gtcgaacggc gcccgtttcg tcgatgaagt cggtctgaaa cgtatttgcg catcggtttt
     6361 cgaacacctg atcaacggcg gccttgagtg atccgagcat gctcgaggag acaatgttgt
     6421 cgagcgattc gccctgcgca ggcgtcggac cgctgccaaa cgtgctgcga tttgcaaaac
     6481 gcaccgtacg ctgccggtcg agcagcagca gtgcgtcggg cgcactgctg gtgaccgttt
     6541 gcagcacgac ctgttgacgc cgcagctgcc gcttgtgacg gtggcctgac cataacaggc
     6601 agccaaatag gatactgagc aatgtcgccg cagcggcaaa tgcaatccac agtctctgcg
     6661 tgatactgcg gctcctcgcg gcgcgtctct cggcgtcgta ggccactttc aacacggcag
     6721 cggcacgcga gcgctgggcc aggtcgagcg aacgctgcag ctccagcgaa tgcgcgagtt
     6781 cccggtacgc gtaatcgaac tgcccgagtg caccgagggc ccgcgcgtag tcaagatgaa
     6841 tcttcggctc ttcggtcgac atgatatacg cggcgcgggg ccctagtact tggtcgagtt
     6901 tggcgagcgc ctgttgcggt tggccgcggg caaggtcgag gcgggcgcgg taagccagca
     6961 tcagcgtgac caggtcttcg cgaccggcgg cagcaaacag agaatcgctg gcactgcaga
     7021 cctgctgcgc ggaatcgaga tcgccggcac tgatcagcgc cgggcacagg gccacattgg
     7081 taaatgacgc tccgaactca tccccgacct ccatggccaa gcggcgagag gactcgaggg
     7141 tttgccgtgc ctcgccgtat ctctgcccct cgatgagaat ttggccgcgt acgtacatcg
     7201 ccgccgccag ctgggacact cgattttcgc tgcgttcaaa ttgcacgacc tcgtccatca
     7261 tccgctccgc ttcccacagc aggccgctgc gtcgaaacgt attggcgaga ctggccgcgg
     7321 cctggatgcg tggttcagtc agtccggcat cttccgcctg actgtatgcc gcgaggccgt
     7381 cggcggccgc gagatcgagt tcgagatgtt cggcgtgtag gtcggccctg gcggcaagcg
     7441 cacacaattg ttcgatcgag cgcgcaggcg tgcgggcgat gattcccgtg atcgactgca
     7501 ttgctgagcg cagattggat tcggtctccg ctgattcaat gtcgttcatg aggagccggt
     7561 cgcgtagtcg ttgggttcca gatgagtccg gctgcgtgtc gagcacgtgc ccagcggcaa
     7621 gcacggctgc gtgtgcttcg ccagatcggc cttgctgcgc ccgcgcagcg gcgatgaccg
     7681 cgtacagctc ggcctcgacg agggcgtcat gccgcagcgg cagcagcgtg agccgccgtt
     7741 ccgcttgcgc gacagctcgt tcgggatcac cgtcggtaag ccggtcaagc agccgcaact
     7801 ccggcgagga tagcggtaca cagtcgccgc gtgcatttcc cgtcaagagg attccgatca
     7861 gcgcgacggc cagaggaaga agccgccctg tgcggtaatt aacttcgaca atctcagcga
     7921 tgccaaagtg catgacaatg cctcgacaat tgtaatcgct ggccacttcg cgcccccgac
     7981 gagttgcaat ggttcggaag tgcgagcaat caagactact ctctaagtcg aagcggcgag
     8041 tcggtttccg agctatcgcg acccggcgcg acgccgcagc cactgattat gtcaatgcca
     8101 cggccctagc gtgcgtgcgc aatcaacagc acattagcga acggcgttcc gcggctcatc
     8161 ggcttcgcct cgctgtcgaa atcgcactcg cgcagcaatt tgcgccattg cgcaacactg
     8221 cggcagtgct gcgtgacgat ggcgtgacca cgaagcagca tgaccagccg gtcgctccac
     8281 tgtccgtagt gaaatcgcaa gccgccgccg gcatcgccga cgcgcagcag caataggccg
     8341 cgcgacggca gcgcagtacg cacccgctgc aacacgtcaa gctgcgcctg cggcgccata
     8401 tagtgcagta catccagtat gaccaccgcg tcgaccgcac cgaatgccgc atcgcgaatg
     8461 tcgccgtgcg acacctcgga cgagggaccg agtgcgcagc gcgcgcgctc gacatcgcgc
     8521 gccatgagtt cgatgccgcg cgtcgacagg gcccgcggcg cgggcggcca agcgcgcggc
     8581 cagctgccgc ggtcgtaaca gcgctccgct gcccgcagcc aagcgctcaa caatccctgg
     8641 ccacaaccaa ggtcgagcac ccgcgcgcgg ccgcgcagta aaccaagttc gagtatggcg
     8701 cggtaaacag gatcggtgcg caatttgccg cgtgcaaaat gatacgcaaa ccggccggcg
     8761 ttgcgatacg gctcgcaggc cgcatcgagc agcgcggcat gcatgcgcga cggcaccgga
     8821 ctctagtgcg ccgcgctacc caggtcgcgg atctccacgc ggcgattgcg cggatcgtcg
     8881 ggccggtcgg gcgccagcaa gtgctcggag ccgaagccga cggtttgcag ccggtccgcg
     8941 cccacgccgt tggcgatcaa gaattgcttc acggcttcgg cgcgcttgta ggacaactgt
     9001 ttgttgtaat cggggttgcc cttcgcgtcc gtgtggccgg caacctcgaa tcgatccttc
     9061 cccaacgagg cgcccgtcaa cgccagttgc agttgcttga gctgcgactc ggccttacgt
     9121 gtgatggcgc tcgaattgtg gtcgaaagga atattgagat tgatggacat cggttgttgc
     9181 ggcggctcag gcggctccgc atcggtccgg gtgaagctgc gcgtcccgaa gccacgagta
     9241 tgcatctgct gcgggtgcag cgcctgttcg atttgttcgc gggtttgcac ggcgctggag
     9301 gcgtccggcg gcgggtcggc cgcctgcgag agcagcggca gacacgcgct ggcaagcccc
     9361 gtcataaata ccacatactt cggcatcatt tgatttgctc caggccctgt gaacggcggg
     9421 cttcgttgta cctatccttc atggcggcga ggttgcgttg cgcgtccgcg ctggcggcgc
     9481 ccggcgcgct gctcttgacc tccaccaagg catcgtaata gcggctgcga gccgccgcca
     9541 acgcgctggc cgaatccgcg gcatccggct cggtgatgat gtcagcagca gacaccgatt
     9601 tcgacagcaa gccggtggcc gagttgccga cggccgagcc gaccaagtcg ctgttcttgc
     9661 cgatgatgtc ggcgagcgca agctgggcct ttttttccga ctccgtttcg gtcaccgcga
     9721 cttcgacggt caccggcatg atttccaacg gtgtgcgcgg caagtccaga tattccgcat
     9781 tgcgcgcatc cagctcggcc ccctgagatt gatactggac cgcgattcgc gtccgctctt
     9841 gcttcaggcc ttcggcggta tcggcgccgg ccgcagcgat gcgttgatcg aggcttgcga
     9901 gcatccgttg atcgcgcgtc attgcgcgtt tcagcgcgtc gatttcgccc atggtttctt
     9961 gctgctgccg cgtcttctcg tcgtagacgc gccgcgcctc gacggagagc ggcggcaccc
    10021 gcaaccaggg tgccgcgtcg cccttggaac cgctcgaatg agatccggcg ctcaccgtgc
    10081 cgatgttcac ccaggcgctc gacagcactt cctgttgatc cgtgacgctc ggggtcgaca
    10141 ccttcaacgt atacagcgtc gtgcgcgcgg cgcccttgtc cgcggtcgtc aacagcgatt
    10201 cgatgtggta gccggcatcc ttcaaccggt aagcggtccc atccttggag aattcaaaac
    10261 gtgcttcgta caccgccttg actttgccga cgacgcacac attggcgcgc cgcaattgat
    10321 tttcgatgga gtcatcggtt cgcgatgtct tccattggtc ttgcggcaag tccttggttt
    10381 cgggcgcgag ctccttgggc agatagtcgg cagtgcagga tgccggttcc ggcttgaagg
    10441 tggcggcgac aatcgtcatg caaccgagct tggcgttgat ggaaagcgcc ggcccggatt
    10501 ggaagtcgac acgatagaga ttcatctggg tgatcacggc gtagcgcgtg tccttgcgcg
    10561 ccgcgcccgc ttttatgcga ttggcggagg cttcgatctc gtgattgacg aatagctcgg
    10621 ccagtttgcc cacgatcagt ccgagcagcg gcaggaacct ggcgttggct tccttgtttc
    10681 cgggcaggaa gtacggttcg tcggcaacga tgcaggtatc gtccacatag gcgcgcactt
    10741 cggggttctg cgcggctttg tcggctgcgg gcggcgcgga ttgcgccgtt tgcaacaacg
    10801 atgcggcgca tgcaaggcaa attagccgac gtgtgcacgc ggcattcacc ggaagcctcc
    10861 cgagcaaccg cgcgaagagt gctttgcaga gcattggaaa cactcagcca ttatgttatt
    10921 tgccgtaatg caacgcacgg ccgcagcggc gcgcgcgtca gggtttttac tttaccccaa
    10981 gtttcgacgg ttcgctcgcc ggccgtaagt tgtacaatgc ggcaagagta agccattacc
    11041 ctggggatat agaatgggtt ttcgcggacg cgtgctgcca ccggcggcgc tgttgttgtg
    11101 ctcgattctc tgccaccccg ccgctgcgcg aaacatcgcc ttgctggtgg gagtcggtac
    11161 gtttcaagcc cccggcttgc agggggccaa gctcctcggg gtgcccaacg atctcgtttc
    11221 gatgcagaag gggctgacgc agcgctgggg atttcagccg ggcgatgtag tcgtgctgca
    11281 cgatcaagac gccacccacg accggatcct cgagcaaatc tcggcgctgc aacagcgcag
    11341 caccgccggc gataccgtcc tcatttatta cagcggtcac ggcaccagcg ccaacgacaa
    11401 tcacaacaag tacgatctgc cgtacgattc tggggcctgg gtgccctacg atgtggacgt
    11461 gagttcgccg aaggcgacac aactctcgtt gatcgtcggt aggcgtgatc tggtaccgcg
    11521 tctgtcggcc ctggacaaag gcggccggtg ggtcgtcgtc gtttccgaca gttgcttttc
    11581 cggacaagta gtgcgcgcat tcggcgagac acacagtacg tcgcgctatc tgccgctgca
    11641 gacccgcgac ctcggtgtct cgcaagcaca acccgctccc gcgccggtgg cggtgacgcc
    11701 gcgaccgacc ccgccgccgt atccctacca gcatgtcttg ctgctttcgg gagccagcga
    11761 cagcgagagc ggcgcggata tttcatctga cgaggggttg cgacaagcgc cgacgatcga
    11821 taatcaatac cacggagcct tcaccgacgc tttcctgcgg ctcatgaacg gacaatttta
    11881 tccggccgga tcgttcaatt acgcgcaggg tcgtcaggca atggtggact ggatggagca
    11941 caatttccag cagcatccgc aattgctccc gaccgtcgct gaagatcctc tgaacgttgc
    12001 ggcgcggccg tttcttgcgc tccagactgc ggcgactgcg gcgccggcag ccggtgttgc
    12061 tgctgctgcg gcgactgcgg cgcgcagcac cacgctgcat gtgaaactcg agtcggtgtc
    12121 gccggcgctg aagacgcaga tcgcgagctt gagcggcgtc gcgctcgtcg atgcgaaacc
    12181 cgatatgacg gtgaaacagc acggcgagca ggtggatttg cgcggcccgg cgggcgatcc
    12241 gatcgtgacc aagccggcga gcgaccctgc gctgatcaag cgcatcagcg cgcaggcatg
    12301 gttgaaccat gtcttgcccg
//
LOCUS       DASWZT010000505        11839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_76492, whole genome shotgun sequence.
ACCESSION   DASWZT010000505 DASWZT010000000
VERSION     DASWZT010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11839
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76492"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VGE92_14515"
     CDS             complement(<1..675)
                     /locus_tag="VGE92_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315090.1"
                     /translation="MPSEVARTGLQLRSLVKASGELELSLTRVPTPTPAANEVLIRVE
                     AAPLNPSDLGLLFAGADLSQASQRGTRREPVVVAKVPEAVLRGMNTRFDVSMPIGNEG
                     AGVVIEAGSSAAAQALKGKTVAALGGAMYSQYRSVPVEQCLVLPAGATAAEGASSFVN
                     PLTALCMIETLRSEGHTALVHTAAASNLGQMLNRICIKEGIALVNIVRSQEQVELLRS
                     QGATFVC"
     gene            828..2180
                     /locus_tag="VGE92_14520"
     CDS             828..2180
                     /locus_tag="VGE92_14520"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY1315091.1"
                     /translation="MGIIRCRTLGAALLIAAAAATAAPETLLRDIANSPQPAQLEATV
                     QTLVGFGTRHSLSDTASPKRGIGAARRWAQSRFEQISRDCDGCLSIVTPSQTVTGARV
                     PKPAEIIDVVAIQKGTSDPERVIVLTGHIDSRVTDPMNATADAPGADDDASGVAVVIE
                     AARILSRHKFPATLVYGVLSGEEQGLYGGKVLADYAKAQGWRVEADLNNDIVGGTRGL
                     NGVVDNTRLRLFSEGTRDEESAEAAKQRRSEGGENDSASRNVARYVKAVAESYLPNWT
                     VALVYRLDRFGRGGDHSAFNAQGFPAVRFTENAEDYRHQHQDLRKEGGVEFGDTIANV
                     DFAYLARVTATNALAAASMALAPPPPDKVKIEGAVTADTRLSWSAAPGSPAQSYRVYW
                     RDTTAPAWSHSRDAGNHETLTLDNMAIDDFSFGVASVAEDGSESPVVFPGPAGAFWAH
                     "
     gene            2180..3406
                     /locus_tag="VGE92_14525"
     CDS             2180..3406
                     /locus_tag="VGE92_14525"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1315092.1"
                     /translation="MASRAFYGWWVVAAAFAVTFIGFGSAYTFSAFIEPLQRDFAASR
                     GSVSLVFSLAGCLYFGLGVLSGPLADRWGSRRLAVAGMLLVGVGLAVASVARTLAEVY
                     LAYGLGVGLGVGCSYVPALGAVQRWFVKRRGFASGLAVSGIGVGTLVMPPLAAFLVGA
                     LGWRHAYLVLGVVAAVVGAGMALLIDNDPRDRGLAPDGDPLEAAVGRAAQAPGFSLRE
                     AIRSRRFAGLYAACLICSFGLFVPFVHLIPYAIDHGVTRSSAVLLLGAVGVGSTGGRF
                     LLGGVADNLGRELALLLMFVGMACAMFVWLFSSLFWEMALFALAYGVCYGGFVAILPA
                     LVMDYFGGRNVSGIIGVLYTSIALGTLVGPSAAGFAFDLSHSYTLPILASVCSNLAAA
                     LTLKVMARPVTAKAVA"
     gene            3403..4161
                     /locus_tag="VGE92_14530"
     CDS             3403..4161
                     /locus_tag="VGE92_14530"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY1315093.1"
                     /translation="MNPGLLVFAAGLIGGTMNALAGGGSFVTLPALISTGMPSVLANA
                     SSTVGMYPGAAASTWAYRDSLGPIGVISLRPLVLVTLVGGAIGAVLLLSTPSTLFDFF
                     LPWLLLLATLTLAFGRPLGERLRRRWHIRPWAVIAVQFAIGIYGGYFGGAIGLMMTAV
                     WRLLDTRDIKSLNGPRTLMVTVANTMAVLIFIAAHAVRWTQTIELLIGAIIGGYCGAL
                     VGRRAPSSWIRAGTLVIASSITGLFFARTYFNFH"
     gene            4182..4922
                     /locus_tag="VGE92_14535"
     CDS             4182..4922
                     /locus_tag="VGE92_14535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY1315094.1"
                     /translation="MKGKPLRGRATAPQHGVARAISKLGLGSRTQAAAWVRDGRVRVN
                     ARVVRDAEFPTRVGIDRIEIDGRAAEAARRCVLMLNKPRGLVTTAQDDQGRATVYDCL
                     AGATLPWLAPIGRLDKASEGLLLFSNDPVWAAGITDPDSGPTKTYHVQINRLPDAALL
                     TALKVGVHSKGESLKASTARALRSGDKNAWLEIVLEEGRNRQIRRLLQALDVSVLRLV
                     RVAIGSLLLGDLAKGRWRLLTQAEIDAL"
     gene            complement(4937..6631)
                     /locus_tag="VGE92_14540"
     CDS             complement(4937..6631)
                     /locus_tag="VGE92_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315095.1"
                     /translation="MFAKKRISSSLHLAMGGFDSASRPGCFCIKLGKPLPFYTAGLRS
                     VSTIPVRYVQWRCAMFNGGLRRALRNRERQSINPANPAMYPPNKSSRSKGHLVFPLLV
                     VMTLTPALRATGWASADIRLSNSGSSPTIQFQVLPNTQKLGVYDSLACEGCSNISSAM
                     FSRNEFKQGQGDQLIQAIEVSTGIKILDGSDATERLNGLVNDQRFGRRLLELIKHDEL
                     RASLDFSAGEFKRPMAPEALLKAVQTGSQIAVPAAKTPIDSLNWLLGYTDLYKFMPRR
                     PAAPEEVIKAHMANVQPRHPLSPSETRALNNSLVKANVIREFIGRLEQGQQLSSVETG
                     VLNRFLLESIYPSETPQMPDALTIAKSIRELQFERHLSAEEMRALLETTYPQETPRTA
                     FERYQSVEDQLPDSVRDGVYIANRHLFPRDGTHIHFAFSNDNKMYYGVPATPGFDLME
                     LDATTQAKRKIAHLHVGDHKVPHLRISTISISPDNRKIALDLTSDPITLFGDSYHDML
                     VLKLGASRTTVFDLGNKAAYGGWWSSASDMFYTSCPLLEHSAPHNDEICAIHLDSD"
     gene            6612..7175
                     /locus_tag="VGE92_14545"
     CDS             6612..7175
                     /locus_tag="VGE92_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315096.1"
                     /translation="MRFLANTIALTSLLAFGGVANAQSETWNWSFTDTVSGGSDSGSG
                     TFTTAALSGASYQVTAVNGTWDSATIAGLIAAGGYAGNDNLLLSGATQLDLPGISFAV
                     DGPSQQVNLAYIAPGEGAVSTGYVVAATNLAGGCESTNCVPTSSFGTFTATQAPEIDP
                     TSAASGLALLLGGVLVLRGRKQQGVAV"
     gene            complement(7321..7908)
                     /locus_tag="VGE92_14550"
     CDS             complement(7321..7908)
                     /locus_tag="VGE92_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315097.1"
                     /translation="MARAAHARSDQAAELEAVNHAMQAHTVDFYNDSLLRFASSGMPS
                     ETTALQQAAFFQGWIGHVGGDGYAHSFLTSQYCTAAAVRNSSIHQQCEALAELLADHG
                     RNTLDVSAAATIGTRLGWASERVSAMREEVLAMFRAENYSGKDPWSCDNVRALTEFAS
                     VQARSGEVAAARAAIQKSGKSISELAKEQTDSIRE"
     gene            complement(8137..8637)
                     /locus_tag="VGE92_14555"
     CDS             complement(8137..8637)
                     /locus_tag="VGE92_14555"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1315098.1"
                     /translation="MRDTSKYVFRETLPSDVEELFSVRARTRENPISKEQLASLGITA
                     ESIAKQMASGRMKGWVCLDSSTLIGFCNGDAETGEVLVLAVLPAYERRGIGTCLLSRV
                     VEWLRSVGFNRIWVAASPDSRIRAHGFYRSLGWRPNGEIDENDDEILEFESGKPLPSD
                     AAGAQS"
     gene            complement(8672..9547)
                     /locus_tag="VGE92_14560"
     CDS             complement(8672..9547)
                     /locus_tag="VGE92_14560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1315099.1"
                     /translation="MQMALATGERDFAVIGCGAIGLTTAVLLARTGARVTIYAKDLPP
                     FTRSSSATGVFTPDSRIALAEYATPAFKNAWNERARYSYRTYQSLLGLADQPVEYLDL
                     YAVNGGQAALGGNEASQGRPAFARLEGELLADLEPKSRTFPAGTHAFGAHGLRRSTTL
                     MFNLSAYTHLLMSDFRSAGGKIEVTEFTSPADFSRLPQKTLVHATGYGARALLNDESV
                     IPVRGQVARTPPQEGIHYALAYRDSIFVPRRDGFVFQVIGNDDYYGYGIDTTAPDRVE
                     AEHAIQTIQSLFPPA"
     gene            10154..>11839
                     /locus_tag="VGE92_14565"
     CDS             10154..>11839
                     /locus_tag="VGE92_14565"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY1315100.1"
                     /translation="MTENFSHGWPPAQESMRRAAAPSPPGGAARTRRGLAVVAVLYTI
                     AAGIVIAIAPQSPRSWRMLPDWIALPVQLMAIASSATVVWHCWRTRAKSLSTWALVCA
                     FCVVSLIANYAWNVWRTWGTPRLSIPDLIYLTDYALMTAAYGVAFVRCGGSFRDPRTW
                     LDAVTIVVALFGTFWATLLGTFLPPSHGPHVDVPYAVAYALSLATWMAMATLLFLRMP
                     KPRPVLVLLIVAGLLDAAWEVGWLATWLTDRNYVGYYYNFGDVLCFMLIACAAALGAA
                     EPEPGKVESAELSSYAFVPALSALLAIALVGATLASTRAPDAWILVGLVMLTTLLLFT
                     RQAAARRKLTELNRALAFRVADARLMEVVRQSADAFLVIDARGIIAFASPATELTIGV
                     APSSLVGTRAATLFGADHAATFERFLGRVIAEPVPPAALELVLESPAQPPRILKLHGA
                     NRLANVHIDGLTLTISDISEQRALERDVLTAANHERLRLAGDIHDGLGQELSGIALML
                     QGLTKLPNLGVAQQQLRTIVGHVADAIRGTRDLARGLSPIYVVRGSLCDALHRL"
BASE COUNT         2087 a         3558 c         3744 g         2450 t
ORIGIN      
        1 gcacacgaac gtggcgccct gggagcgcaa cagctcgacc tgctcctgac tgcgcacgat
       61 gttcaccaag gcaatgccct ccttgatgca aattcggttg agcatttggc cgaggttcga
      121 ggctgcggcg gtgtgcacca gggcggtgtg gccttcgctg cgcagggtct cgatcatgca
      181 cagcgcggtc aacggattga cgaaggacga ggctccctcg gcggcggtgg cgccggcggg
      241 caacaccagg cattgctcga ccggcacgct gcggtactgc gaatacatgg caccgccgag
      301 cgcggcgacg gtcttgccct tgagggcctg cgccgccgcc gatgaaccgg cttcgatgac
      361 gacgcccgcg ccttcgttgc cgatgggcat ggacacatcg aagcgggtat tcatgccgcg
      421 aagcacggcc tcgggaacct tcgccaccac gaccggctcg cgccgcgtgc ctcgctggct
      481 cgcttggctc aagtcggccc cggcaaacaa caggccgaga tcggaggggt tgaggggtgc
      541 cgcctccacc cggatcagca cctcgttggc ggcgggcgtc ggcgtgggca cgcgggtcag
      601 ggacaattcg agttcgccgc tcgccttgac gagcgaacga agttgcagac cggttcgagc
      661 gacttctgat ggcatggcgt attcctcaat tcccgtggcg gtgcgcgatg ctacaccggc
      721 gtttaggtaa gcggcgagtg ggcgcgcccg attgccgcgc ctaagggcgt taccaggtag
      781 aatttccgcc ccaagaacgc aacttgtccg accctcagga ttcgattatg ggaattatcc
      841 gctgccgcac gctcggcgcg gccctgctga tcgccgcggc cgcggccacg gcggcgcccg
      901 agaccttgct gcgggacatc gcgaactcgc cgcagcccgc gcagcttgag gccacggttc
      961 aaaccctggt cggcttcggc acgcgacact ccctgtccga taccgcctcg cccaagcgcg
     1021 gcatcggcgc cgcgcggcgt tgggcgcagt cgcgtttcga gcagatcagc cgcgactgtg
     1081 acggctgctt gagcatcgtg acgccctcgc agacggtcac cggcgcgcgc gtgccgaaac
     1141 cggccgagat aatcgatgtg gtggcgatcc agaagggcac gagcgatccc gagcgggtca
     1201 tcgtgctgac cggacacatc gattcacggg tcaccgaccc gatgaatgcg acggcggatg
     1261 cgcccggggc cgacgatgat gcgtcgggtg ttgcagtggt catcgaggcg gcgcgaatct
     1321 tgagccgcca caagttcccg gccaccctgg tgtacggtgt gctgtcgggc gaggagcaag
     1381 gcctgtacgg tggcaaggtg ctcgcggatt atgccaaggc ccagggctgg cgcgtggagg
     1441 cggatcttaa caacgacatc gtcggcggta cgcgcgggct gaacggggtg gtcgataaca
     1501 cgcggctgcg tttgttctct gagggcaccc gcgatgagga gagcgccgag gccgccaagc
     1561 agcggcgctc cgagggcggc gagaacgatt cggcgtcgcg caatgtggcg cgctacgtca
     1621 aagccgtcgc ggaatcgtat ctgccgaact ggacagtcgc gctcgtgtat cgtttggacc
     1681 gcttcggccg aggcggtgat cactccgcgt tcaacgcgca gggctttccg gcggtgcgct
     1741 ttaccgagaa tgccgaggac taccgtcacc agcaccagga tcttcgcaag gagggcggcg
     1801 ttgagttcgg tgacacgatt gccaacgtcg atttcgcgta tctcgcgcgt gtcaccgcca
     1861 ccaatgcgct cgcggccgca tcgatggcct tggcgccgcc gccgccggac aaggtcaaaa
     1921 tcgagggcgc ggtcaccgcg gatacgcgac tgtcgtggag cgcggcgccc ggatcgccgg
     1981 cgcaaagcta tcgtgtgtat tggcgcgaca cgaccgcgcc cgcgtggtcg cactcacgcg
     2041 acgcgggcaa tcatgaaacg ctcaccctcg ataacatggc catcgatgat ttttcattcg
     2101 gcgtcgccag cgtggcggag gatggatccg agagcccggt ggtgtttccg gggcccgcgg
     2161 gagcgttctg ggcgcattga tggcttcacg cgccttctac ggttggtggg tcgtggccgc
     2221 cgcttttgcc gtgaccttca tcggcttcgg cagtgcctac acgttcagtg ccttcattga
     2281 gcccctgcag cgggatttcg ccgcctcacg cggatcggtg tcgctggtct tctcattggc
     2341 gggatgcctg tacttcggcc tgggagtcct cagcgggccg ctcgccgacc gctggggttc
     2401 acggcgactc gccgtcgccg gcatgttgct ggtgggagtc ggcctcgcgg tggcgagcgt
     2461 cgcaaggacc ctcgccgagg tttacctcgc ttatggtttg ggcgtgggat tgggggtcgg
     2521 ctgctcctat gtgccggcgc tcggcgccgt gcagcgttgg ttcgtcaagc ggcgcggctt
     2581 tgcatcgggg cttgcggtga gcggcatcgg cgtcggcacg ttggtgatgc cgccgctcgc
     2641 ggccttcttg gtcggcgctc tcggctggcg tcacgcctac ctcgttctcg gcgttgtcgc
     2701 cgcagtggtg ggcgcgggca tggcgctgct gatcgacaac gacccgcgcg atcgagggct
     2761 cgcgccggac ggggaccccc tcgaggcggc ggtgggccgt gccgcgcagg cgccgggctt
     2821 ttcgctgcgc gaggcgatcc gctcgcgacg cttcgccgga ctctatgcgg cgtgcctgat
     2881 ttgctccttc ggcctgttcg ttcccttcgt gcatctcatt ccgtatgcga tcgatcacgg
     2941 ggtgactcgc tcctcggcgg tgttgttgtt gggcgcggtc ggggtgggca gcaccggcgg
     3001 acggttcttg ctcggcggcg tcgccgataa tctgggccgc gagcttgcac tgctcttgat
     3061 gttcgtcggc atggcgtgcg ccatgttcgt gtggctgttc tcatcgctct tctgggagat
     3121 ggcgctgttt gccttggcct atggcgtgtg ctatggcggc ttcgtggcaa tcctgccggc
     3181 gctggtgatg gattatttcg gcggacgcaa tgtgagcggc atcatcgggg tgctgtacac
     3241 cagcatcgcc ttgggcactc tggtcggccc gagcgcggcc ggcttcgcct tcgacctaag
     3301 ccacagctac acgctgccga ttctggcgag cgtctgcagc aatctggcgg cggcgctcac
     3361 gctcaaagtc atggcgcggc ccgtcaccgc gaaggcggtg gcgtgaatcc tggcttattg
     3421 gtgtttgctg ccgggctcat tggcggcacg atgaatgccc tcgccggcgg cggatcgttc
     3481 gtgaccttgc ccgcgctcat ctccaccggc atgccgtcgg tgctggcgaa cgcatcgagc
     3541 accgtcggca tgtatccggg tgccgcggcg agcacttggg cctaccgcga tagcttggga
     3601 cccatcggcg tcatctcgct gcggccgctg gtgctcgtga ccctcgtcgg cggcgccatc
     3661 ggcgcagtat tgttgttatc cacgccctcg acgctgttcg atttctttct gccgtggctg
     3721 ctgctgctcg cgaccctgac gctggcgttc ggccggccct tgggcgagcg gctgcgacgg
     3781 cgctggcaca tccggccgtg ggcggtgatc gcggtgcaat tcgcgatcgg gatctacggc
     3841 ggttacttcg gcggcgccat cggcctcatg atgaccgccg tctggaggct gctcgatacg
     3901 cgcgacatca agagtctgaa cggaccgcgc acgctgatgg tgacggtcgc caacaccatg
     3961 gcggtgctga ttttcatcgc ggcacatgcg gtgcgttgga cgcaaaccat cgagcttctg
     4021 atcggcgcca tcatcggcgg ctattgcggt gctttggtcg gacggcgcgc accctcgtcg
     4081 tggatacgcg cggggacctt ggtgatcgcg agcagcatca ccgggctgtt ctttgcccga
     4141 acatacttta actttcattg actaaagatg aactgcgctc aatgaaaggc aagccacttc
     4201 gcgggcgggc aacggcgccg cagcacggcg tggcgcgagc gatttcaaaa ttgggcttgg
     4261 gctcgcgcac gcaggctgcc gcgtgggtgc gcgacggccg ggttcgcgtc aatgcgcgcg
     4321 tggtgcgcga tgcggaattc ccaacgaggg tcggaatcga tcgcatcgag atcgacgggc
     4381 gggcggccga agccgctcgt cgatgcgtgc tgatgctgaa caagcctcgg ggcttggtga
     4441 cgacggcgca ggatgatcag ggccgcgcga cggtgtacga ctgtcttgca ggtgcgacat
     4501 tgccctggct cgcgcccatc gggcggctcg acaaggcgag cgagggactg ctgttgttca
     4561 gcaatgaccc cgtctgggcc gccggcatca ccgatcccga ctcggggccg acgaagacct
     4621 atcacgtgca gatcaaccgc ttgccggatg cggcattgct cacggcactt aaggtgggcg
     4681 ttcactcgaa aggcgagtcc cttaaggcaa gtacggcacg cgccttgcgc agcggcgaca
     4741 agaacgcgtg gcttgagatc gtgctcgagg aagggcgcaa ccggcagatt cgccgcttgc
     4801 tgcaagcgct cgatgtgtcc gtgctgcgct tggtgcgggt ggccatcggc tcgttgttgc
     4861 tgggcgatct ggcgaagggc cgatggcggc tgctgacgca agcggagatc gatgcgctgt
     4921 gaacgcggct tgttgctcaa tctgagtcca aatggatggc acagatttca tcgttgtgcg
     4981 gcgcgctgtg ttccaagagt gggcaggacg tgtaaaacat gtcggacgcc gaactccacc
     5041 agccaccgta cgcggcttta tttccgagat cgaatactgt tgttcttgag gcgcctaact
     5101 tcaaaaccag catgtcatgg tagctgtctc caaatagagt aatggggtcc gaggtcagat
     5161 ctagcgcaat ttttctattg tcgggcgaaa tcgatatcgt gctaattctc aagtgaggga
     5221 ccttatggtc gccaacgtgc aagtgggcaa ttttccgttt tgcttgcgtc gtggcatcga
     5281 gctccattaa atcaaaacca ggcgttgccg gtactccgta atacattttg ttgtcattag
     5341 agaatgcaaa atgtatatgc gtgccatctc tggggaacaa atgtctattc gcgatataca
     5401 cgccgtctcg taccgagtct ggaagctgat cttcaacgct ttggtacctt tcgaaagctg
     5461 tccttggcgt ctcctgtggg taagtggtct ccaacaaagc ccgcatttct tcagcgctta
     5521 agtgcctctc gaactgtaat tcccgaatgg acttcgcaat cgtcaatgca tctggcattt
     5581 gcggagtttc tgacggatag attgactcga gcaagaatct attcaggacc ccggtttcca
     5641 cgctactcag ctgctgtccc tgttctaatc gaccaataaa ctctctaatg acatttgctt
     5701 tcacgaggga attatttagc gctcgtgttt ctgaaggact taaaggatgt cgcggctgca
     5761 cgttggccat gtgggcttta atcacctctt cgggtgcagc aggtcgtctt ggcatgaact
     5821 tgtacaggtc tgtataaccc aatagccaat tcaaggaatc gattggagtc tttgcagcag
     5881 gaacagcaat ttggcttcct gtctgtaccg ccttgagcag agcctcggga gccatggggc
     5941 gtttgaattc tcccgccgag aaatccaagg aggctcttag ttcatcatgc ttgatcaact
     6001 ccagcagccg ccgcccaaat cgctggtcgt tgaccaagcc gtttagcctt tccgtggcat
     6061 cagatccatc caggatcttt atcccggttg atacttcaat ggcttgtatt aactgatccc
     6121 cctgtccttg cttgaactcg ttccgtgaaa acatcgcaga actaatgttg ctacagcctt
     6181 cacaagcgag gctgtcgtag acgcccagct tttgcgtgtt gggcaaaact tgaaattgga
     6241 tcgtgggtga ggatccagaa ttagacaatc taatgtccgc agacgcccaa cccgtggccc
     6301 gaagggcagg ggtgagggtc attaccacca ataaagggaa cacgagatga cctttagacc
     6361 ttgaggattt gttaggtgga tacatggcag gatttgcagg attgatcgat tgccgttcgc
     6421 gattgcgcaa tgcccgccgc aagccgccat tgaacatagc gcaccgccat tgaacatagc
     6481 gcaccgggat ggttgacacc gatcgtaatc ctgctgtgta aaacggcaat ggtttgccca
     6541 gtttgataca aaagcagcca gggcgagatg ctgaatcaaa cccacccatt gctaaatgta
     6601 aggaactcga aatgcgcttt ttagcgaaca cgattgcatt gacatctctg ctggccttcg
     6661 gcggagttgc gaacgcccag tcggaaacct ggaactggtc gttcaccgat accgtatcag
     6721 gcggaagcga cagtggctcg ggcactttta caaccgctgc gctctcagga gccagttatc
     6781 aggtcacggc tgtgaacgga acgtgggatt cggcgacgat tgccggtctt atcgccgcgg
     6841 gcggttacgc ggggaacgac aacctgctgc tcagcggcgc aacgcaactg gacttaccgg
     6901 ggatatcgtt tgcagtagac gggccgagcc agcaggtgaa tttggcgtac atcgcaccgg
     6961 gagagggtgc cgtgtctacc gggtacgtgg tggcggccac gaacctggcc ggcgggtgcg
     7021 agagcaccaa ctgcgttccc acttcaagtt tcggtacttt cactgcgacg caggcacccg
     7081 aaatcgaccc cacctcagcc gctagtgggc tggcgctgct ccttggtggc gtcctcgtac
     7141 tgcgtggtcg caagcagcaa ggcgtcgctg tttaatctga cgactgtttg agctgagaga
     7201 cccggccgtt caggcctcgc cggtccaaaa gacccgcgag gccttctttt taccagcgca
     7261 gtacagaaaa gcccaatggc aatcggttcc gaaaggtcat cgccggtcac ccgagcagcg
     7321 ctattcgcga attgaatcgg tttgctcctt ggccagctcc gagatcgatt tgcccgactt
     7381 ctgaatcgca gcacgggccg ctgcaacttc accggatcgt gcctgaacgc tggcgaactc
     7441 ggtcaaggcc cgcacattgt cgcagctcca ggggtccttg cccgaatagt tctcagcccg
     7501 aaacatggcc aatacttcct cgcgcatggc gctaacgcgt tccgacgccc aaccgagacg
     7561 tgtgccgatc gtagctgccg ccgacacatc gagcgtattg cgaccatgat ccgccagcaa
     7621 ctccgctagc gcctcacact gctgatggat cgaagagttc cggaccgcgg ccgccgtaca
     7681 atattggcta gtcaagaatg agtgagcata gccatctcca cccacgtggc cgatccagcc
     7741 ctggaaaaat gccgcttgtt ggagcgctgt cgtctccgat ggcataccgg aacttgcgaa
     7801 gcgcaacaac gaatcgttgt aaaaatcgac cgtgtgagcc tgcatcgcgt ggttcaccgc
     7861 ctcgagttcc gctgcttgat ccgatcgagc gtgagcggcg cgggccattt caagccacgg
     7921 agcagcgtta tcctagtccc agcccgtgct ttgtagcaga agccccgccg cgcgttcgtg
     7981 atagtcatcg ctatcgatga gcgaccgacg ccagtgggat tgcgcgaacg atccatcgtc
     8041 ggggcgcttt cataatagtt ccgctttcac gctatccaga atgcaaaatc gcgtgcggat
     8101 cgattggagc tttggccagg ccgagggtat ctgtactcag ctttgcgcac cagctgcatc
     8161 gctcggtaac ggtttgcctg actcgaactc aaggatttca tcatcatttt cgtcaatttc
     8221 accattcggc cgccatccca gggaacggta aaagccatgc gctcgtattc tcgagtccgg
     8281 tgaagccgcc acccatatcc tgttgaagcc gacagatcga agccactcaa cgacccgcga
     8341 gagaaggcaa gtgcctatac ctctgcgctc gtacgccggc aatacagcga gcacgaggac
     8401 ctcgccggtt tccgcgtcgc cgttacagaa gccgataagc gttgacgagt ccaaacacac
     8461 ccatcctttc atccgaccgc tggccatctg cttagcgatt gactcggcag ttattccaag
     8521 cgatgcaagc tgctctttag agatcgggtt ctctctggta cgggcacgaa ccgagaagag
     8581 ttcctctaca tcggaaggta acgtttcgcg gaacacatac ttgctcgtgt cgcgcattcg
     8641 gcgcaactcc ttctgctgtt tgtagaggcg ctcacgccgg tggaaatagc gactggatcg
     8701 tctgaatcgc gtgttcggcc tcgacccgat caggcgcggt ggtgtcaatg ccgtagccgt
     8761 aataatcgtc gttgccaatc acttggaaaa caaagccgtc acgacgcggg acgaagatgc
     8821 tgtcgcggta cgcgagggca tagtgaatcc cttcttgtgg aggtgtgcgc gcaacctgtc
     8881 cgcgcaccgg aatcaccgac tcgtcgttga gaagagcgcg ggcaccatag ccggttgcat
     8941 gaacgagtgt tttctgcgga aggcgcgaga agtcggcagg cgaagtgaac tctgtgacct
     9001 cgatcttccc gccggcggac cggaagtccg acatcaacaa atgcgtgtag gcgctcaggt
     9061 tgaacattaa cgttgtggag cgcctcagcc cgtgtgctcc gaaggcatgc gtcccggccg
     9121 ggaacgtgcg gctcttgggt tcgaggtcgg cgagtagctc gccttcgagt cgggcgaagg
     9181 cgggccggcc ctggctggcc tcattgccgc ccaatgcggc ctgtccgccg tttacggcgt
     9241 acaagtccag gtattcgact ggttgatctg ccagcccaag gaggctttgg tacgtccgat
     9301 acgaatagcg ggctcgctcg ttccaggcat tcttgaacgc gggcgtggcg tactcggcca
     9361 gtgcgatgcg cgagtccggt gtgaacacgc ccgtggccga ggaggagcgc gtgaagggcg
     9421 gcaggtcttt ggcgtatatc gtgactcgcg cgcccgtgcg cgcgagcagt acggcggtgg
     9481 tgagcccgat cgccccgcag ccaatgaccg cgaagtcgcg ttcgcctgtg gcgagcgcca
     9541 tctgcattgc ttcgtggccc gatccccagg aaagcgacca gccgctgccg ccgtgtccgt
     9601 agtgatgaat cacggtcttg tcgcctcggc gttctccctc gagtcgcgga ccctgcgctc
     9661 ggaagggtcg tgtacacacc gtgatcgccg agatccgatc cggtgcaatt cgcagtggtg
     9721 gaagtatgag ttgcgaaaga ctggagggcc gccacgtgtc aagcgcgcaa ccgcccaaag
     9781 caccgacgac ggccgcggct ccggctcggg tcagaaaatc tcggcgcaga atggatgaag
     9841 ccatcccatg gacctcccgg ataccgtcct cgggtgtgaa acgagtaatg attctagcgt
     9901 ctctgacttg cagaggatgt tcgttacggg ccccgaagcc taccgcaaac cgcgggatca
     9961 ccggactcga attctcctac gccggcctcg cggcaacgcc attgttgcag gtctaagcga
    10021 ttgttaatgc aaggattgtt cgatattgcg ctgcgtccgt atcgagtagt ggcagtatgt
    10081 tgcccaacgg cacttagcgt ccctcgacct cgaatacaag aacgccatca ttaaagagca
    10141 accccactcc tcggtgacgg agaatttttc tcacggatgg ccgccggcgc aggagtcgat
    10201 gcgccgtgcc gccgcgccga gcccaccggg cggtgcagcc cgcacccggc gtggcctcgc
    10261 cgtggtcgcg gttctgtaca caatcgccgc gggcatcgta attgcaatcg caccgcaatc
    10321 gccgcggtcc tggcggatgt tgccggactg gatcgcgctc cccgtgcaat tgatggccat
    10381 agcaagttcg gcgacggtcg tctggcattg ctggcgcacg cgcgcgaaat ccttgagcac
    10441 ctgggcgctg gtgtgcgcat tctgcgttgt gagcctgatc gcgaactacg catggaatgt
    10501 gtggcgcacc tggggcacac cccggttgtc gattcccgat ctgatctacc tgacggacta
    10561 cgcgctgatg accgcagcct acggcgttgc attcgtgcgt tgcggcggat cgtttcgcga
    10621 cccgcgaacc tggctcgatg ccgtgaccat cgtggtcgcg ctttttggga cgttctgggc
    10681 aacgctgctc ggtacatttc ttccgccatc gcatggcccg cacgtcgatg tgccgtacgc
    10741 ggtggcttat gcgctttcgt tggcgacgtg gatggcgatg gcgacgctgc tgtttctgcg
    10801 catgccaaaa ccgcggccgg tcctggtatt gctcatcgtt gccggcctcc tcgacgcagc
    10861 ctgggaagtg ggctggctcg ccacgtggct cacggaccga aactatgtgg gttactacta
    10921 taactttggc gacgtgctgt gttttatgct gatcgcatgc gccgcggcgc tcggcgccgc
    10981 ggagccagaa cccggcaagg tcgagagcgc ggagctttcc tcgtatgcgt tcgtgccggc
    11041 attgtcggcg ctgctcgcaa ttgcactggt cggtgcgacg ctcgccagca cccgggcgcc
    11101 cgacgcctgg attttggtgg gtctcgtcat gctgacgacg ctgctcctgt tcacccggca
    11161 ggccgccgca cgccgtaaac tgaccgagct caatcgcgct ctggcatttc gcgtcgccga
    11221 tgcacgcttg atggaagtcg tgcgtcaatc ggcggacgcc ttcctggtca tcgacgcgcg
    11281 cgggatcatc gcgttcgcga gtccggcaac ggagctcacg atcggtgttg cgccttcgag
    11341 cctcgtcggt acgcgcgccg ccaccttgtt tggcgcggat catgccgcaa ccttcgagcg
    11401 tttcctcggg cgggtcatcg cggaaccggt gccgccggcg gcgctcgaac tggtcctcga
    11461 atcgccggca cagccgccgc gtattctgaa gctgcacggt gccaatcggc tcgcgaacgt
    11521 gcatatcgac ggactgacgc tgacgatatc cgacatatcc gaacagcggg cgctcgagcg
    11581 cgatgtgctg accgcagcca accatgagcg tctgcgcctc gccggggata tccacgacgg
    11641 gctcggacaa gagctctcgg gaatcgcgct gatgctacaa ggcctgacga agttgccgaa
    11701 cttgggcgtc gcgcaacagc agttgcgaac catcgtcggt cacgtcgcgg atgccatccg
    11761 cggcacgcgc gatctggcgc gcggcctgtc gccgatctat gtggtgcgcg gatcgctgtg
    11821 cgacgcgttg catcggctc
//
LOCUS       DASWZT010000506         4039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_52403, whole genome shotgun sequence.
ACCESSION   DASWZT010000506 DASWZT010000000
VERSION     DASWZT010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52403"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="VGE92_14570"
     CDS             complement(<1..1130)
                     /locus_tag="VGE92_14570"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315101.1"
                     /translation="MYRIFLSSPGDVRHERVRAQVVIDRLNAEHPAEPIFSLTRWEES
                     YYIATGTFQDQISSPGQHDLVVFIFWKRFGTDLPAPYNRADGTSRTGTEYEFEDARDA
                     RERRADHLPDILVYRKTAKVLFSEENLESERAQKKSLDQFWERWFRSDTGHYIAGFQS
                     FPDTADFERQFERNLREWLRRRHAGAVSWNVAKQGSPYRGLAPYEEGHAGLFFGRDLD
                     MGRARARFIEAAVGQESGRRGTPFLLILGATGSGKSSFLRAGLVPYMRSLGVPAFLED
                     GSDAIYAFRTLTLMPRELGENLCSGLAAALYRSNSPGARGETGLPQLAQGDYPTSEGF
                     AALAAGSPQSACAPVVRALDRAAGEGQEDHPGGALRRVGLLLA"
     gene            complement(1832..1990)
                     /locus_tag="VGE92_14575"
     CDS             complement(1832..1990)
                     /locus_tag="VGE92_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315102.1"
                     /translation="MAEFASISGAAAVPPVMVGVPQGGPKVQDVAVNLLPMKSNSVPL
                     MEQGLLLL"
     gene            complement(2172..2588)
                     /locus_tag="VGE92_14580"
     CDS             complement(2172..2588)
                     /locus_tag="VGE92_14580"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEY1315103.1"
                     /translation="MQTDYISRDTYGMYKNIDSGPGPSLMGADTLLGNDVYNSQNESL
                     GTIKEFMIDMASGRIAYAVLSFGGFMGMGDRLFAVPWQALQLDTVNKRFTLNVSKDKL
                     KNAPGFDKDRWPSMGDTSWATGVHSFYGVKHTGRIQ"
     gene            2815..3081
                     /locus_tag="VGE92_14585"
     CDS             2815..3081
                     /locus_tag="VGE92_14585"
                     /inference="COORDINATES: protein motif:HMM:NF013035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine rich repeat-containing protein"
                     /protein_id="HEY1315104.1"
                     /translation="MKRSTLVFSMCIAALTRFAVADEPPAPPQPRGGEVRAACEADVK
                     KLCADVAPGGGRVMQCLKEHKEEVSDGCKQAIMKARQGAADNKS"
     gene            3123..>4039
                     /locus_tag="VGE92_14590"
     CDS             3123..>4039
                     /locus_tag="VGE92_14590"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEY1315105.1"
                     /translation="MATAKTKATRARKPAARKRARGKLAPGGQSRGLEVADIAIGLDS
                     ADIADLAALIRKAGGALIGAYRDPLGGRPLLLASLPLNAVQPTPFQRDLSPTHAKRLA
                     TKIDETAAFLDPLIVVRGDEGRFWTPNGRHRLAAGKVLGLKQITALISPDETLAYRIL
                     ALNTEKAHNLRDRSLEVIRMARNLAKRHPADKEIEYAAQFEAPELLTLGLVYEKSSRF
                     AGGAYSSFLKKVERFSERTLAASLRERDGFAARLLAIDAQAKRIIAALQARGFKSPYL
                     RNYVVARINPVRFHKVKKGDTQPPMPLAQA"
BASE COUNT          805 a         1239 c         1221 g          774 t
ORIGIN      
        1 gccaacaaca gtccaacgcg tcgcaacgcg ccgcccgggt gatcctcctg accctcgcca
       61 gccgcgcggt cgagtgcacg aacaacgggc gcacaagcgg attgcgggct gcccgccgcg
      121 agcgcggcaa agccctccga cgtcggatag tcgccttgcg caagctgcgg cagtccggtc
      181 tcaccgcgcg cccccggcga gttcgaacgg tacagggcgg cggcaagacc ggaacacagg
      241 ttctcgccga gttctcgagg catgagggtg agggtgcgaa acgcgtaaat ggcatcgctc
      301 ccatcctcga ggaaggcggg caccccgagc gagcgcatgt acggcacgag ccccgctcgg
      361 aggaaggacg acttgcccga acccgtggcg cccaagatga gcaaaaacgg cgtaccccgg
      421 cggccgcttt cctgtccgac cgccgcctcg atgaagcgtg cccgtgcccg acccatgtcg
      481 agatcgcgac cgaagaacaa tcccgcgtgt ccctcctcgt agggtgcgag gccgcggtag
      541 ggcgagccct gtttggcgac attccagcta accgcgccgg cgtggcggcg gcgcagccat
      601 tcgcgcaaat tacgttcgaa ttggcgttcg aaatctgcgg tgtcggggaa gctttggaag
      661 cccgcgatgt agtgccctgt gtcgctgcga aaccagcgct cccagaactg atccagcgac
      721 ttcttctgcg cccgttcaga ttctagattt tcctcgctaa acaacacttt agccgtctta
      781 cggtaaacca ggatgtctgg caggtgatcg gcgcggcgct cgcgcgcatc gcgcgcatcc
      841 tcgaactcgt actcggtgcc ggtacgggaa gttccgtcgg cgcggttgta gggcgccggc
      901 agatccgtgc cgaagcgctt ccagaatatg aaaacgacga ggtcatgctg gccggggctc
      961 gaaatttggt cctggaaagt accggtcgca atgtagtacg actcctccca gcgggtgaga
     1021 ctgaaaatcg gctccgccgg atgttcggca ttgagacggt cgatcacgac ttgggcgcgc
     1081 acgcgctcgt gccgaacatc gccgggcgag gacaggaaaa ttcggtacat caggtgagca
     1141 ttacatattc aaaatgtttt tttggtgctc tcttgtgacc acctggcaga tgcggtattc
     1201 aatgacaatg atattgcaat cgtcgtcgcc caaagcgacg cacggcactt aaacaaacag
     1261 ggattttcgc aggggtattt atgaagcgtt ctcggatctt ggctgctgtt gggctgttgt
     1321 ctttggtcac atcggccgcc tacgcgaaag gcatcgcata tgacggaaag acaacggtag
     1381 ggtcgggaac ggctacatca ataaacctga cagggacgag ctctggttgc gccggtgttc
     1441 tcgttgagag tgtcgaccct ctgggttgcc tccccgggat tacctcgctg agctttacgc
     1501 ccggcgatac cgcgggatcg agcagctaca cggaaatcgt gagcgaaagc gcttacaccg
     1561 catttctctg gccggcgtcg agtgagagtg cgttcgactc aaattataac ggggcttttg
     1621 cacagaccga tgtcgtagcg gcggtgattt cgggcgcggg cacgggcgat tgtgcggcga
     1681 gtagcaacgc gaattgctgg tcgatcgaat tcgactacga tagcgtttcg aaggctggcg
     1741 gtatgccgcc ggtcatcact ccgttgacta gttgcccggg tacctatggc gcagcgtccg
     1801 tctcgatcgt cgcgggcgga gtcactacga cttatagcag cagtaaccct tgctccatca
     1861 acggcaccga attggatttc atcggcagca aattgaccgc cacgtcctgt accttcggac
     1921 ccccctgcgg cacaccgacc atgacgggcg gcacggccgc cgcaccggag atggatgcga
     1981 attccgccat ctccgcgctg acactgttcg cgggttgctt ggcggtattt tgcgcagtcc
     2041 ctcgcccacc ccggcaacgg gcgatggtgc gcctgccagc ttgcaagcga gccgttgatt
     2101 agcaagcctg ccgtcgatta agaagagggc tggcgcttgg cgccggccct cggccttaag
     2161 tatagtttcc tctactgaat tcgcccggtg tgtttcaccc catagaacga gtggacaccc
     2221 gtcgcccatg acgtgtcgcc catggagggc cagcggtcct tatcgaagcc gggcgcattc
     2281 ttcaacttgt ccttgctgac attcagcgta aagcgtttgt tcacggtgtc gagctgcagc
     2341 gcctgccacg gaaccgcgaa cagccgatcg cccattccca tgaagccgcc gaacgacagc
     2401 acggcgtagg cgattcgtcc cgatgccatg tcgatcatga attccttgat cgttcccaag
     2461 ctctcatttt gggagttgta gacatcgttg ccgagaagcg tatcggcgcc catcaatgag
     2521 ggtcccggcc cgctgtcgat attcttgtac atgccgtagg tatcgcgtga aatgtagtct
     2581 gtctgcatga gtgtgtctcc aatggatggg aatgttgaat ctagagacgg caaggttttg
     2641 ccgttcaagt gatcctgact atccgcggat cgcgccaaga tatgtgtcgg ccggtttctg
     2701 cagagtcggt ttcagaaaag aaccgctcgc tgtcggcatt tgctcacact tgcgccccgc
     2761 atggcgccct cgaaccgcaa gcagtatatt ggcgcggcca cataggagat ccccatgaag
     2821 cgctcaaccc tggtcttcag catgtgcatt gccgcactga cacggttcgc ggtcgccgat
     2881 gagccccctg cgcctcccca gccgcggggt ggtgaggtgc gcgccgcctg cgaagccgac
     2941 gtcaaaaaac tctgcgccga cgtagctccc ggcgggggcc gcgtcatgca atgcctcaag
     3001 gagcacaagg aagaggtgtc ggacggctgc aagcaggcga tcatgaaagc gcgccagggt
     3061 gcggccgaca ataaatccta attaatgccg gatcgcgcgc agagcaaatt cgatccgcat
     3121 tgatggccac ggcaaaaacc aaggcgacac gcgcgcgtaa acccgcggcc cgtaagcgcg
     3181 cccgcggaaa attggcgccg ggcggccagt cgcgcggcct cgaagtcgcc gacatcgcga
     3241 tcggcctcga cagcgcagac attgccgatc tcgccgccct gatacgcaaa gctggcggcg
     3301 cactcatcgg cgcgtatcgc gatcctttgg gcgggcggcc tttgttgctc gcgtcgttgc
     3361 cgctcaatgc agtgcagccg acgccgtttc agcgcgatct gtcgcccacg cacgcgaagc
     3421 gactggcaac gaaaatcgac gaaaccgcgg cgttcttgga tccgttgatc gtagtgcgcg
     3481 gcgatgaagg ccgcttctgg acgccgaacg gccgccatcg cttggccgcg ggcaaggtgc
     3541 tcgggctcaa gcagatcact gcactgatct cgcccgacga aacgctggcc tatcgcatcc
     3601 ttgcgctcaa taccgaaaag gcccacaacc tgcgcgatcg cagcctcgaa gtcattcgca
     3661 tggcacgaaa cctcgcgaag cggcatcccg ccgacaaaga aatcgagtac gcggctcaat
     3721 tcgaagcgcc tgaattgctg accttaggtc tcgtgtatga aaagtcgtcg cgcttcgccg
     3781 gcggcgcgta cagttcgttt ttgaaaaaag tcgagcgatt cagcgaacgc accctcgccg
     3841 caagcttgcg cgagcgcgat ggctttgccg cgcggctgct cgcgatcgac gctcaggcga
     3901 aacggatcat cgctgcgctg caggcgcgcg gcttcaaatc gccgtacctg cgcaactacg
     3961 ttgtggcgcg catcaacccg gtgcgattcc acaaagttaa gaaaggcgac acccaaccgc
     4021 ccatgccctt ggcccaggc
//
LOCUS       DASWZT010000507         4409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_47704, whole genome shotgun sequence.
ACCESSION   DASWZT010000507 DASWZT010000000
VERSION     DASWZT010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47704"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VGE92_14595"
     CDS             complement(<1..218)
                     /locus_tag="VGE92_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315106.1"
                     /translation="MASTIQQFLKEHGISEVEAIIPDMAGVARGKLMPAEKFAEEEGM
                     RLPEAIFLQTVTGDYPDDQSAMNPSDID"
     gene            complement(228..974)
                     /locus_tag="VGE92_14600"
     CDS             complement(228..974)
                     /locus_tag="VGE92_14600"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEY1315107.1"
                     /translation="MSRLPLIGIPADRRLCGKHYFHMAGEKYIEAVAIGANAVPVLIP
                     ALGSEIDLPSLLEACDGLLLTGSASNVEPHHYGGPASEPGTLHDPNRDATTLPLIPRA
                     VDAGLPVLAICRGFQEMNVAYGGTLYPRLHEVAGYFDHREDESTPLEVQYGPAHEVLL
                     EPGGALRKIAGLDRLQVNSLHWQGIRTLGRELAVEARAPDGVIEAFRVASAANFALSV
                     QWHPEWQFAQNPFSRALFAAFGDASRARTV"
     gene            1222..2532
                     /locus_tag="VGE92_14605"
     CDS             1222..2532
                     /locus_tag="VGE92_14605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY1315108.1"
                     /translation="MRLSYPPAASYYAASVASSARLSVEGPRPHRETLRGEHRFDVVV
                     LGGGIAGCSAALHLAQRGYRVALLEARVVGYGASGRSGGQTIFGLAASQARLTAEVGR
                     EAARRLFELSIEALDLTQALIGQYGIDCDYHANHVHVAVKPRHLDELQEWARELHEDY
                     HYTSARLLNRDELQQHVRSERYLGGLLDSRSGHLHPLKYTQGVAHAAELAGAEIFENS
                     PVLRYTDGAEVTVHTAHGSVRCAHLVLCGNAYLGAVAPSLARRILGVGTYIIATEKLG
                     EDRVRALLPSNAAIADINWILDYFRRSADHRLLFGGRVSYSAVQPPRLADSMRRRMLR
                     IFPGLAGIKVAYSWGGFLDITMSRAPNFGRLASNVFYMQGFSGHGMSLTGLAGKLVAE
                     AIAGTAERFDVFARIPHRDFPGGPLFRRPSLVMAMLYYRLRDLL"
     gene            complement(2546..2926)
                     /locus_tag="VGE92_14610"
     CDS             complement(2546..2926)
                     /locus_tag="VGE92_14610"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HEY1315109.1"
                     /translation="MLAQLSGAAAPAWAHHSFAMFEPIKSFTFKATVKTFQWTNPHVI
                     LWILVQPDGGGAAQEWSIETTSPGVLTRSGWTRLSIKPGDRVSVTFSPLRDGSHGGQL
                     HSVTLLDTGQTLLPSYGREQPGLK"
     gene            complement(2959..3801)
                     /locus_tag="VGE92_14615"
     CDS             complement(2959..3801)
                     /locus_tag="VGE92_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315110.1"
                     /translation="MLLRTSSNLALAAMICACLALPVLAAVLGAEPDTINPAPTAQDW
                     QRLAKLPDWSGIWTPVISDQVAQEKGNRPPWRPQIAKQIEQMYAEEAAGRPFPIIDHC
                     FPTGMPSWMLITHNAFEVLFTPGRVTLLGEGDGNRLRRIYTDGRPHPADPDPSFHGHS
                     IGHWENDTLVVDTVGILPEVYLAISEAVGVPNNGDMHIVERLRLKDADTLADDLEISA
                     HKVLTEPWRTTRKYYRQRAQKYDIVEGVCEQGSFSEAVDKNGNSIFAPLKFHQGVPVG
                     SDSK"
     gene            complement(3811..>4409)
                     /locus_tag="VGE92_14620"
     CDS             complement(3811..>4409)
                     /locus_tag="VGE92_14620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY1315111.1"
                     /translation="VNFRDYLDTGLFLDHRIVRDMLRRWAQDADFLNLFCYTGSATVY
                     AAAGGARRSASVDLSNTYLEWARQNLLLNGFDAARHELFRADCLQWLEERAASGPMFD
                     LIFVDPPTFSNSKRMEGALDVQRDHVGMIRRSMQLLRPTGRLVFSTNYTRFKLDSQAL
                     ADLNVEDISAQTIPKDFERHARIHRCFVIRCHSVPGLR"
BASE COUNT          829 a         1373 c         1319 g          888 t
ORIGIN      
        1 atatcgatat cggacgggtt catggcgctt tgatcgtccg gatagtcgcc ggttaccgtc
       61 tgcaagaaaa tcgcctcggg caggcgcatg ccctcctctt cggcaaattt ctccgcgggc
      121 atcagcttgc cgcgcgccac acccgccatg tccggaatga tggcttcaac ttctgaaata
      181 ccgtgttcct tcaagaactg ctgaatcgtg ctggccataa aacaccttta aactgtacgc
      241 gctcgactgg catcgccaaa cgccgcaaac agcgcgcgcg aaaatggatt ctgtgcaaac
      301 tgccattccg gatgccattg cacgctcagg gcgaaattcg cggcgctggc gacccggaaa
      361 gcctcgatga ctccgtccgg tgcgcgcgcc tcgaccgcca attcccttcc caaggtgcga
      421 attccttgcc aatgcaggga attgacttgc aggcgatcca atccggcgat tttgcgcaat
      481 gcccctccag gttcgaggag aacctcgtgg gcaggcccgt actgcacttc gagcggcgtc
      541 gattcatctt cgcgatgatc gaagtaccct gccacctcgt gtagccgcgg gtataaggtg
      601 ccaccatagg caacgttcat ttcctgaaat ccgcggcata tggcgagcac cggcaagccg
      661 gcgtcgacgg cgcgcggaat cagcggaagc gtcgtagcgt cccgatttgg gtcatgtagt
      721 gttcccggct cactcgccgg tcctccgtaa tgatgaggtt cgacattcga tgcgctcccg
      781 gtcagcaaca atccgtcgca tgcctccaac agcgagggca agtcgatctc ggacccgaga
      841 gcgggtatca gcaccggtac cgcgttggcg ccgatggcaa ccgcctcgat gtacttctcc
      901 ccggccatgt ggaaatagtg tttcccacac agcctgcggt cggctggaat tccgatgagc
      961 ggcaggcgag acatgtttaa tatattaaac gtccagatgg acgattgggc ttccttaacg
     1021 ctcgtattac accacaccac cgccaactcg tcccgtccga ggtgacacat ttagctaaga
     1081 attaggcatt tccacgactt acaggtgctt tgtgcgatgc gtcaacattg cgttatgatc
     1141 aaaaaatccg gtaggctcac aggcgcccaa tcctgtcaat agccataaga ttttaaacac
     1201 tctgctgcca cttgagccct cgtgcgtctg tcctacccac ccgctgcttc ctactacgcg
     1261 gcctcggttg cttcctcggc gaggttatcg gtagaaggtc cccggccgca tcgtgagaca
     1321 ctgcgcggcg agcatcgctt cgacgtcgtg gtgctcggcg gcggcatcgc cggttgttct
     1381 gctgcactgc atctggcgca gcgcggttat cgcgttgctt tgctcgaggc gcgggtcgtc
     1441 ggctacgggg catccgggcg cagcggcgga caaacgatat tcggcttagc cgcgagccaa
     1501 gcgaggctca ccgccgaagt cggccgcgag gcggcgcggc ggctgttcga gctgtcgatc
     1561 gaagcgctgg atctgacgca agcgctgatc ggccaatacg gcatcgattg cgactatcac
     1621 gccaatcacg ttcacgtggc cgtcaaaccg cgccatctcg atgagttgca agaatgggcg
     1681 cgtgaactgc acgaggacta tcattacacc tccgcgcgac tcttgaatcg cgatgagcta
     1741 cagcagcatg tgcgcagcga gcgttacttg ggcggcctcc tcgactcgcg cagcggccat
     1801 ctgcatcccc tcaagtacac gcaaggggtt gcgcatgccg cggaattggc gggcgccgag
     1861 atctttgaga attctccggt gctgcgctat accgatggcg ccgaagtcac ggtacacacc
     1921 gcacacggat cggtgcgttg cgcgcacctg gtgctgtgcg gaaacgccta tctcggcgcc
     1981 gttgcgccct cgctggcacg tcgcatattg ggcgtgggta cctacatcat cgccaccgaa
     2041 aagctcggtg aagaccgggt gcgcgcgctg ctgccgagca atgccgcgat cgccgatatc
     2101 aactggatct tggattattt ccgccgctcc gcggatcacc gcttgctatt cggcggccgc
     2161 gtgagctaca gcgccgtaca gccgccgcgc ttggcggatt cgatgcgccg gcgcatgttg
     2221 cgaatctttc cagggctcgc cggcatcaag gtggcgtatt catggggcgg cttcttggac
     2281 atcaccatga gccgcgcgcc caatttcggg cggctcgcct ccaatgtttt ctatatgcag
     2341 ggcttttccg gccacggcat gtcgttgacc gggctcgcgg gcaagctggt ggccgaagcc
     2401 atcgcgggaa ccgcggagcg cttcgatgtg ttcgcgcgta tcccgcatcg cgacttcccc
     2461 ggaggtccgt tgttcaggcg gccgtcattg gtgatggcca tgctgtatta ccgtctgcgc
     2521 gacttgctgt gatccgcgtc gatggctatt tgagtcccgg ttgctccctg ccgtagctcg
     2581 ggagtaacgt ctgaccggtg tcgagcaagg tcacggaatg caattggccg ccgtgcgagc
     2641 cgtcgcgcag cggactgaag gtcacgctta cccggtcgcc cggtttgatc gacaggcgcg
     2701 tccatccgga gcgcgtcagg acgcccggac tggtggtctc aatcgaccac tcctgcgccg
     2761 cgccgccgcc gtccggctgc accagtatcc acaggatgac gtgcggattg gtccactgaa
     2821 acgtcttgac ggtggccttg aacgtgaaac tcttgatcgg ctcgaacatc gcgaaggaat
     2881 ggtgcgccca cgccggtgcc gcagcgccgg acaactgagc caacatgatc gcgcaccatg
     2941 cccgaagcgc tgcgtgcttc atttcgaatc ggaccctacg ggcacgccct ggtgaaactt
     3001 gagcggtgcg aaaattgaat tgccattctt gtcgactgct tcgctaaaag agccctgttc
     3061 gcaaacgcct tccacgatgt cgtacttctg agcccgctgc cgatagtatt tgcgcgtcgt
     3121 gcgccatggt tccgtcaaca ctttatgggc gctgatctcg agatcatcgg cgagcgtatc
     3181 ggcatccttg aggcgcaaac gctccacgat atgcatatcg ccattgttcg ggacgccgac
     3241 ggcctcggaa atcgccagat agacctcggg caagatcccc accgtgtcca ccaccagcgt
     3301 gtcattttcc caatgcccga tcgaatggcc gtgaaagctc ggatcggggt cggccggatg
     3361 cggccgtccg tcggtataaa ttcgccgcag cctattgccg tcgccctcgc ccaagagcgt
     3421 gacgcggcca ggcgtaaaca gcacttcgaa ggcgttgtga gtgatcagca tccagctcgg
     3481 catgccggtc ggaaaacagt ggtcgatgat gggaaaaggg cgtcccgcgg cctcctccgc
     3541 atacatttgc tcgatttgct tcgcaatctg cggtctccac ggcgggcggt tgcccttctc
     3601 ctgcgcgact tgatcgctga tcaccggggt ccagatcccg ctccaatccg gcagcttggc
     3661 caggcgctgc caatcttgcg cagtcggcgc agggttgatg gtatccggct ccgcgccgag
     3721 caccgcggcg agcacgggca gcgccaggca tgcgcaaatc atcgctgcca gagccaggtt
     3781 cgaggaggtg cgcagtagca ttgcgcaaga ctagcgcaag cctggcacgg agtgacaccg
     3841 aatcacgaag cagcgatgaa tgcgcgcatg ccgctcgaaa tccttaggaa tggtttgggc
     3901 gctgatatcc tcgacattga ggtcggcgag cgcctgcgaa tcgagcttga aacgggtgta
     3961 gttggtcgag aataccaacc ggcccgtcgg ccgcagcagc tgcatcgagc gccggatcat
     4021 cccgacgtga tcgcgctgca cgtcgagggc gccctccatg cgtttggagt tggagaacgt
     4081 cggcgggtcg acgaagatca aatcgaacat cgggccgctt gccgcgcgtt cctcgagcca
     4141 ctgcaggcaa tccgcgcgga atagttcatg ccgcgccgca tcgaatccgt tgagcagcag
     4201 attctgtcgc gcccactcca ggtaggtgtt cgacaagtcg acgctcgcgc tgcggcgcgc
     4261 tcccccggcg gcggcatata ccgtggcgct gccggtgtag cagaatagat tcaggaagtc
     4321 cgcatcctgc gcccagcgac gcagcatgtc gcgcacgatc cggtggtcca gaaacagccc
     4381 ggtgtcgaga tagtcgcgga aattgaccc
//
LOCUS       DASWZT010000508         3897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_14006, whole genome shotgun sequence.
ACCESSION   DASWZT010000508 DASWZT010000000
VERSION     DASWZT010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14006"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1865)
                     /locus_tag="VGE92_14625"
     CDS             complement(<1..1865)
                     /locus_tag="VGE92_14625"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY1315112.1"
                     /translation="MAKFRVARIKLIRWGVALSVVCAVLAAVGIAWMVHLDHLVTEEF
                     QGRHWSVPARVYAAPLELYVGAPVSAGDLEEELLRLHYRAGDPAAGPGLYRRHGNSFD
                     VRARRVRFIDELREAELVSIGADANAITSLKQADAALPVFRLDPPVIGSVSPIHGEDR
                     LILSPADVPPLLREGIKLIEDRRFDEHPGVDVRGVMRALWVDLRARRVVQGGSTLTQQ
                     LVKNYFLSDEQSVGRKATEALMALRLETHFSKQEILVAYLNEVYLGQDGARAIHGFGL
                     ASEYYFAKPLAELDVGELATLVGLVKGPSYYDPRKHPDRALARRNLVLQEFGAAHLID
                     AAAAKRAAATPLKVKPPGGAYVPAFLDLVRRHLKRDYADADLAAAGLAVYTSLDPRAQ
                     SAAERALTQNLKRLDAASRVRGASLEGAVIVTEPNSGDVVAVVGGRDAGTDGFDRALD
                     AHRPIGSLVKPAVYLTALETGRYNAATLIEDAPLELKLKDGSVWAPQNFEHQFYGQVP
                     MARALAESMNLATVRLGLELGLPKVAATLQRLGLDTAPTLNPSMLLGTVEMTPLEVVE
                     VYTSLANGGFRARLRAVLAVVDERGRPLKSFKVQVEEAAPAAAVFALDRMLTLVTT"
     gene            1914..2729
                     /locus_tag="VGE92_14630"
     CDS             1914..2729
                     /locus_tag="VGE92_14630"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY1315113.1"
                     /translation="MSIPNIPAIIYGTAWKKSDTARLVSTAIQLGFRGIDTACQPKHY
                     DEAGVGAGVAACLGAALDRADLYLQTKFTPLSGQDPARVPYDASAPLPLQVTQSCAAS
                     LENLQTRYLDCLVLHSPLANAQQTLEAWRAMESLIAAGTVLNLGISNCRAGQLAALCA
                     AARVKPAIVQNRWVKETHYDRDIRAFCRQQQILYQSFWTLSANPHVLADAAILTLAAK
                     YGRTPAQIVFRYLTQSEVIPLSGTRSAAHMREDLAVFEFELSAHERAAVGDIF"
     gene            2801..>3897
                     /locus_tag="VGE92_14635"
     CDS             2801..>3897
                     /locus_tag="VGE92_14635"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1315114.1"
                     /translation="MSPNRLLAAAALAVLWAAAARAETFHYRGDVQVTATSGAGCSGA
                     RPYTIHNVDLMVRDDAPGMRYEGFVGGQGLSVRRIAGARLDQLSVTVAVSDPKLAARF
                     RLSLSALGSDALSGHTEAASVPADTPGCYIDTADLNLSRVAGPAGDYLQRAAMQFHIL
                     QLQAQWATLWQEKDYGAALPVARQAEALAEQSFDAADARLAPSLVRLIATLMCSGNAP
                     EAAVQLRRAFALDGRSPVRNQQILGELTQTTVAAHKYAEFESLLRQTIALEEKATPPA
                     DLATYLNYLGYLLQSASRYADAEPVLRRALRLDEQTLQPNDPQLAYVVSQLGQDLMYT
                     GQYAESEQLLRRAVAIAEKTTPPVYLGDYLNT"
BASE COUNT          674 a         1304 c         1249 g          670 t
ORIGIN      
        1 gtcgtcacca gcgtcagcat ccgatcgagc gcaaataccg cggccgccgg tgcggcctcc
       61 tcgacttgca ccttgaagct cttgagcggc cgaccgcgct cgtcgacgac cgccagcacg
      121 gcccgcaggc gtgcgcgaaa cccgccattc gcgagcgaag tgtaaacctc aaccacttcg
      181 agcggcgtca tctccacggt gcccaagagc atggacgggt tcagtgtcgg cgcggtgtcg
      241 agtccgaggc gttgcagcgt agcggccacc ttgggcaggc cgagctcgag tccaagccgc
      301 accgtcgcga gattcatcga ctctgccagc gcgcgcgcca tcggcacctg gccataaaac
      361 tgatgttcga agttctgcgg tgcccacacg ctgccgtcct tgagcttgag ctcgagcggt
      421 gcatcctcga tcagcgtggc cgcgttgtaa cggccggtct cgagcgccgt gaggtacacc
      481 gccggcttca ccagcgagcc gatcggccgg tgcgcatcga gtgcgcgatc gaatccatcc
      541 gtgcccgcat cgcggccgcc gaccaccgcc accacgtcgc cgctgttggg ctcggtgacg
      601 atgaccgcgc cttcgaggga ggcaccgcgc acgcggctcg cggcatcgag acgcttcaag
      661 ttctgcgtga gcgcgcgctc ggcggccgac tgcgcgcgcg gatcgaggct cgtgtacacg
      721 gcaaggcctg cggccgccag atccgcgtcg gcgtagtcgc gcttcagatg gcgccgcacc
      781 agatcgagaa aggccggcac gtaggcgccg cccggcggct ttaccttgag cggcgtggcg
      841 gcggcgcgtt tcgcggccgc ggcgtcgatc aggtgcgccg ccccgaactc ctggagcacg
      901 agattgcgcc gcgccagcgc gcgatcgggg tgtttgcgcg gatcgtaata cgagggccct
      961 ttgacgagcc cgaccagcgt tgcgagttca ccgacatcga gttccgccag cggcttggcg
     1021 aagtagtact cgctggcgag tccgaagccg tgaatcgcgc gcgcgccgtc ctgtccgaga
     1081 tacacctcat tgaggtaggc gacgaggatc tcctgtttgg agaaatgcgt ctcgagccgc
     1141 agtgccatca gcgcttcggt cgccttgcgg ccgacacttt gctcatccga cagaaagtag
     1201 tttttcacca actgctgcgt gagggtgctg ccgccctgta cgactcggcg ggctcgcaag
     1261 tcgacccaca atgcgcgcat gacgccgcgc acatcgacgc ccgggtgctc gtcgaagcgc
     1321 cggtcttcga tgagtttgat gccctcgcgc aacagcggcg gcacgtccgc gggcgacagg
     1381 atcagccggt cctcgccatg aatcggagag acgctgccga tgacgggcgg atcgaggcgg
     1441 aacacgggca gcgccgcgtc cgcctgtttg aggctcgtga tcgcattcgc gtccgcgccg
     1501 atcgagacga gctcggcctc gcgcagctcg tcaatgaagc gtacgcggcg cgcacgcaca
     1561 tcgaaggagt tgccgtgccg ccggtaaagc ccggggcccg ccgccggatc gccggcgcgg
     1621 tagtgcagac gcagcagttc ctcctcgaga tcgcccgccg aaaccggtgc accgacgtac
     1681 agttccagag gcgcggcgta gacgcgcgcc ggcaccgacc agtgccgccc ttggaattcc
     1741 tcggtgacga ggtgatcgag atgcaccatc caggcgatgc cgaccgccgc gagcactgcg
     1801 cacacgacgc tgagcgcgac gccccagcga atcaatttaa tgcgcgctac gcgaaattta
     1861 gccaaaattg cccttgatct ttgcggtcgc atagtttgac ggggtgactc atcgtgtcaa
     1921 taccgaacat cccagcgatc atttacggca ccgcctggaa gaaatcggac acggcacgcc
     1981 tggtcagcac ggcgattcaa ctgggatttc gcggcatcga caccgcctgc cagccgaagc
     2041 attacgatga ggcgggcgtg ggcgcgggtg tcgccgcctg tctcggcgcg gcactcgacc
     2101 gcgcggatct gtacctacaa acgaaattca cgccgctgtc tggtcaggac ccggcgcggg
     2161 ttccctatga tgcgagcgcg ccgttgcccc tgcaggtcac tcagtcttgc gccgcgtccc
     2221 tcgagaattt gcagacccgg tacctggatt gcctggtgct gcactcgccg ctcgccaacg
     2281 cgcaacagac gctggaggcg tggcgcgcca tggagtcgct gatcgccgcg ggcacggtgc
     2341 tcaatctcgg gatcagcaac tgtcgcgccg ggcagctggc ggcgctgtgc gcggcggcgc
     2401 gcgtcaagcc ggcgattgtg cagaaccgct gggtaaagga gacccattac gaccgcgaca
     2461 ttcgcgcctt ctgccggcag cagcagattc tctaccagag cttctggacg ctcagcgcca
     2521 atccacacgt tctcgccgac gcggccatcc tcaccttggc cgcaaaatac ggccgcactc
     2581 ccgcgcaaat cgtcttccgc tatctgacgc aaagcgaggt catcccgttg agcggaaccc
     2641 gctcggccgc gcacatgcgc gaggatctgg cggtgttcga gttcgagctg tcggcgcatg
     2701 agcgagccgc ggtgggtgac atattctaag atgagtactg ctggctctcg gacctctgag
     2761 gcatatactt ctgcttgtcc aactcgcacg gtcgccgtcc atgtcgccga atcgcttgct
     2821 tgccgccgct gcgctggcgg tgctgtgggc cgccgccgcg cgcgccgaga cttttcatta
     2881 tcgcggcgac gtacaggtaa ccgcgacctc cggcgcgggt tgcagcggag cgcgacccta
     2941 cacgatccac aatgtcgacc tgatggtgcg ggatgacgcg ccaggaatgc gctatgaagg
     3001 cttcgtcggc ggccaaggtt tatccgtgcg aaggatcgcc ggcgcccgac tcgatcagct
     3061 cagcgtcacg gttgcagtgt ccgatccgaa gcttgccgct cgattccgcc tgtcgctgag
     3121 cgctctcggg agcgatgcgt tgagcggaca caccgaggcg gcctcggtgc ccgcggatac
     3181 gcctggttgc tacattgaca cggccgattt gaatctgtcc cgcgtggccg gcccagcggg
     3241 cgattacttg cagcgggccg cgatgcagtt tcacatcttg cagctgcagg cgcagtgggc
     3301 gaccctgtgg caggagaaag actacggcgc cgcgctcccg gtcgcgcggc aggccgaagc
     3361 gctcgcggag cagtctttcg atgccgccga cgcccgcctc gcgccgtcgc tggtgcgatt
     3421 gattgcaacg ctcatgtgct ccggcaacgc gcccgaggct gcggtacagc tgcgtcgtgc
     3481 gtttgcactg gatggccgca gcccggtgcg aaaccaacaa attctaggcg aattgaccca
     3541 aaccactgtg gctgcacaca agtacgccga gttcgaatca ctgctgcggc agacaatcgc
     3601 ccttgaagaa aaagcgacac ccccggcgga tcttgccacg tacctcaact atttgggcta
     3661 tttgctgcag agtgcctcgc gctatgcgga cgccgagccc gtgctgcggc gggcccttcg
     3721 cctcgatgag cagacgctgc agccgaacga tccgcaactg gcgtacgtcg tgagtcagct
     3781 gggccaagat ctcatgtata ccggccagta cgccgaatcc gagcagctgc tgcggcgcgc
     3841 cgtggcaatc gccgaaaaga ccacgccgcc cgtctatctt ggcgactacc tcaacac
//
LOCUS       DASWZT010000509         2919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_57320, whole genome shotgun sequence.
ACCESSION   DASWZT010000509 DASWZT010000000
VERSION     DASWZT010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57320"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="VGE92_14640"
     CDS             <1..1176
                     /locus_tag="VGE92_14640"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEY1315115.1"
                     /translation="REVLLGLFFITVGMLLDVGLLLRHFPLVAATLIGMLLLKAAVVT
                     VAAEPATKSWFKALRTGVIVSQGGEFGFALLTLLLRRELLDPGVVQPLLAATVLSMVL
                     SPLLIKHNRRITRALLSESGPPDSAAMRETQATLAVAERDHVVICGFGRVGQNIARVL
                     EQTGFEFIALELDAFRIRAGRQAGDPVIYGDAGQVKVLENVGVAHASCVVITFANPDV
                     ALRILRAVRELRADVPILVRTQDDTKLEQLQSAGATEVVPETFEASLMLLSHLLLLLK
                     LPVARVIRTVNDIRSHRYSMLRQYFRAADAEHLDASHAFREELHSVVLPPHAWAVGRS
                     ITELTARGSRATVSAVRRDGIVGREPGADTMFKEGDVVVVCGTPEAVEHAETLLLMG"
     regulatory      1225..1320
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1419..2777
                     /locus_tag="VGE92_14645"
     CDS             1419..2777
                     /locus_tag="VGE92_14645"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY1315116.1"
                     /translation="MVSKVTRSPGADSSGVYRRPRLVLKLKRFEDLPSVLSKTKSYPT
                     PIRMVGADYSQTRCVGGDGGTTVETDALDAVIEYGETFVRAQAGVRLGTLVRALAERG
                     LELPLTPEMGQISLGALAVTTLPQASYGAHMAQLSSCVTELKLITPQGKQMTVTERQA
                     ALMRVLRSSFGLLGVVHEVVLRVRPLTPVKIDYQVLTLKEFTARFSAIAGGPGALRLH
                     ISPFNDRITVERRTLDESASLSRSGIWQIRNSVMRNVLPAFGSTVGSVLAAPGLRAVM
                     LSGMQRALRATLDRSTRGVAMYSHEWMRDLPSEAWRARHTYSLWAFPQAAYPKVLAEY
                     FAFCRSYYKEHRYRCNVVSGASRLHQDRGSLFSASYSGAAFTLEPSSTGEAGWDDFLI
                     DFNDFASRLNGTPTFNQTRALQPEHVSKAFGERVKLFRALRHRTDPLNRLRNSYFAHL
                     LG"
BASE COUNT          500 a          922 c          920 g          577 t
ORIGIN      
        1 cgcgaggtat tgctcggcct ttttttcatc accgtcggca tgctgctcga cgtcggcttg
       61 ctgctgcgcc attttccctt ggtggcggcg acgctgatcg gcatgctgct gctcaaagct
      121 gcggtggtta cggtcgccgc cgagcccgcg accaagagct ggttcaaagc gctgcgcacc
      181 ggcgtcatcg tcagccaggg aggcgagttt ggcttcgcgc tgctgacgct gctgctgcgc
      241 cgcgaattgc tcgatccggg cgtggtgcag ccgctgctcg ccgccaccgt tctcagcatg
      301 gttctgtcgc cgctgctcat caagcacaat cgccgcatca cgcgcgcgct gctgagcgag
      361 tccggaccgc ccgacagcgc ggcgatgcgc gaaacccagg cgaccctggc ggtcgccgaa
      421 cgcgatcacg tcgtgatctg cggctttggc cgcgtcggcc agaatatcgc ccgggtgctc
      481 gaacaaaccg gcttcgagtt cattgccctg gaactcgatg ccttccgcat acgcgccggc
      541 cggcaggccg gcgatccggt catctacggc gatgccggcc aggtcaaagt gttggagaac
      601 gtcggcgtcg cgcatgcgag ctgcgtggtc atcacatttg ccaaccccga tgtggcgttg
      661 cgaatcctgc gggcggtgcg cgagctgcgc gcggatgtgc cgattctggt gcgcacgcag
      721 gacgacacca agctcgaaca attgcaatcg gccggcgcaa ccgaagtggt accggaaacc
      781 ttcgaggcca gtctcatgct gttatcgcac ttgctgctgt tgttgaagtt gccggtggcg
      841 cgcgtgatcc gcacggtcaa cgacattcgc agccatcgct acagcatgtt gcgacagtac
      901 tttcgcgccg ccgatgcgga acatctcgac gccagccacg cgtttcgcga agaacttcac
      961 agcgtcgtcc tgccgccgca cgcgtgggcg gtggggcgca gcatcaccga gttgactgcc
     1021 cgcggatcgc gggcgacggt gagcgccgtg cgccgcgacg gcatcgtcgg gcgcgagccg
     1081 ggtgccgaca ctatgttcaa agagggcgac gtggtggtgg tctgcggcac cccggaagcg
     1141 gttgaacacg ctgaaacgct gctcttgatg ggctgaaaaa aattgccctt tacagcgacc
     1201 gcgcatccat cgcatagtag cgatgctgtt caagggcaaa cccgccgaaa ggtggggacg
     1261 caaaaccacc ggtctaaggg acttccctac aagaagtccg acgatagcgg ggtcgccagt
     1321 atcgaagcgc tataattgcg cattgcgaga ccttccggtt ttgcgaaccc ccgacagcag
     1381 tggaaagccg attatttcaa gtctttcggg gtgtgcacat ggttagtaaa gtcactcgtt
     1441 cgcccggtgc ggattcctcg ggcgtttatc gccggccacg gctggttctc aaactcaagc
     1501 gcttcgaaga tcttccgagc gttctttcca agacaaaaag ttatccgacc cccatccgca
     1561 tggttggcgc cgattactcg cagactcgtt gcgtcggcgg tgacggcggc accaccgtgg
     1621 agacggacgc gctcgacgcc gtcatcgaat acggcgaaac cttcgtcaga gcgcaggccg
     1681 gcgtgcgcct ggggaccttg gtgcgtgcgc tcgccgagcg tggtttggag ctgccgctga
     1741 ctcccgaaat gggtcagatt tctttgggcg cgctcgctgt aaccaccttg ccgcaagcct
     1801 cgtacggcgc gcatatggcg cagctgtcgt cctgcgtcac ggagctgaaa ctcatcactc
     1861 cgcaaggcaa gcagatgacc gtgaccgagc ggcaagccgc gctgatgcgg gtgctgcgct
     1921 cgagcttcgg tttattgggc gtggtgcatg aagtggtgct gcgtgtgcgg ccgctgacgc
     1981 ccgtcaaaat cgattatcag gtgctgacgc tcaaggagtt cactgctcga ttcagcgcca
     2041 tcgcaggcgg ccccggcgca ctgcggctgc acatctcgcc tttcaacgat cgcatcaccg
     2101 tggaacgccg caccctcgat gaatcggcgt cgctgagccg ctcgggcatc tggcaaattc
     2161 gcaattccgt gatgcgcaac gtgctgccgg ccttcggttc caccgtcggc agcgtgctgg
     2221 ccgcgcccgg cctgcgcgcg gtcatgctgt ccggcatgca gcgggcgctg cgtgcgacgc
     2281 tcgaccgcag cacgcgcggc gtggcgatgt actctcacga atggatgcgc gacctgccga
     2341 gcgaggcgtg gagggcgcgc cacacctaca gcctgtgggc ttttccgcag gccgcgtacc
     2401 ccaaggtgtt ggccgaatat tttgcctttt gccggtctta ctacaaagaa caccgctacc
     2461 gttgcaacgt cgtgagcggc gcgagccggc tgcatcagga ccgcggttcg ttgttcagtg
     2521 ccagttattc aggcgctgcg tttacgctcg agccgtcgtc caccggcgag gccggctggg
     2581 acgatttttt gatcgacttc aatgactttg catcgcgatt gaacggcacg ccgaccttca
     2641 atcaaacgcg tgccttgcag ccggaacatg tgtcgaaggc attcggcgaa cgcgtcaagt
     2701 tgttccgtgc attgcgccac cggacggatc cgctcaaccg cttgcgcaac agctactttg
     2761 cgcacttgct gggctaggct atagaccgga aacgatcttg gctttgatcg actcgaattc
     2821 cgagtcgctg atgagcttgg cgtcgagcat ctcgcgcgcc tgccgcagcc gccgcgccgg
     2881 ttccgattcc gccgccgcgg cgtccgcacc ggcataggc
//
LOCUS       DASWZT010000510         1652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_38017, whole genome shotgun sequence.
ACCESSION   DASWZT010000510 DASWZT010000000
VERSION     DASWZT010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38017"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="VGE92_14650"
     CDS             <1..716
                     /locus_tag="VGE92_14650"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEY1315117.1"
                     /translation="LELAEPAVRTGVWEHSRFRQDPIGRIQRTGLAAMITVYGPRSVA
                     EPMIAGVCRMHAAVRGETLAGKPYSAQDPALLTWVQTTAIYGIAQAYSRYVTVLSPDE
                     FDALYRESAPASRLYGAHGAPTSANETEILFESMRGRLEPSPIVLEFLRIMSEKIALP
                     SPLQWLQPVLVRAAVEIVPAWIRGRLGLTEVYGLAPHERWLVRRAGNLSNRIVLAESP
                     PARSCRRLGLSMNHLYARL"
     gene            complement(713..>1652)
                     /locus_tag="VGE92_14655"
     CDS             complement(713..>1652)
                     /locus_tag="VGE92_14655"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY1315118.1"
                     /translation="YTTPATATSDNGAAFQVTVKNAAGSVSSNVADLTVMAVAAPPTA
                     APFRHVVIVLEENQNASSVVGSSEMPYLNSLIRDYGLATQYYANTHPSIGNYFMLTTG
                     QVLTNDDNETPSSFPVAANNVVRQLLISGKSFKAYAENLPSAGYTGGDSGNYAVRHVP
                     LAFLTDVQDSPSEKLNLVPFTQFAADLAAGELPDYSFVTPNLCDDAHNCALGVADAWL
                     QTHIEPLLKSSAFKNDGLLIIVFDESESDNSHGGGRVPAILASPAFSKAGYQSTTFYQ
                     HSSTLRLMLEGLGVKGLPGAAATAPTMWEFFTFPPP"
BASE COUNT          297 a          510 c          529 g          316 t
ORIGIN      
        1 ttcttgaact ggccgagccg gccgtgcgca cgggcgtgtg ggagcattcc cgattccggc
       61 aagatcccat cggccgcatc cagcgcacgg gcctcgcggc catgatcacc gtctatggcc
      121 cgcgcagcgt tgccgaaccg atgatcgcgg gtgtttgccg aatgcacgcc gccgtgcgcg
      181 gcgaaacgct cgccggaaag ccctactcgg cgcaagaccc cgccttgttg acttgggtgc
      241 aaacgacggc gatctatggc attgcgcaag cctacagccg ctacgtgacg gtcttaagcc
      301 cggatgaatt cgacgcgctg tatcgcgaat cggcgccggc atcacggctc tacggtgcgc
      361 acggtgcgcc gacatcggca aacgaaaccg aaatcttgtt cgagtcgatg cgcggccgct
      421 tggagccatc gccgatcgtg ctcgagtttc tgcgcatcat gagcgagaag atcgcactgc
      481 cctctcctct gcaatggctg caaccggtgc tggtgcgcgc cgccgtcgag atcgttcccg
      541 cctggattcg cgggcgtctc ggtctgaccg aggtctacgg attggcgccg cacgagcgct
      601 ggctggtgcg gcgcgccggt aacttgtcca accggattgt gcttgcggag agtccaccgg
      661 cgcgatcctg tcgacgcttg gggctctcga tgaaccatct gtacgcgagg ctctaagggg
      721 gcgggaaagt aaagaattcc cacatcgtgg gcgccgtcgc cgcggcaccg gggagcccct
      781 tgacgcccaa gccctcgagc atgagccgca gcgtgctcga atgctgatag aacgtcgtcg
      841 attggtagcc tgcttttgag aaggcggggc tcgccagaat cgccgggacg cgcccgccgc
      901 cgtggctgtt atcgctctcg gactcatcga agacgatgat cagcaggcca tcgttcttga
      961 acgcgctgct cttcagcaac ggctcgatgt gagtctgcaa ccaggcgtcc gcgaccccga
     1021 gtgcgcagtt gtgggcatcg tcgcacagat tcggcgtcac gaacgaatag tcgggcagct
     1081 cacccgccgc gaggtcggcc gcgaactgcg tgaagggcac caaattcaat ttctcgctgg
     1141 ggctgtcctg tacatccgtc agaaatgcca gcggcacgtg ccgcaccgcg tagttgccgg
     1201 aatcaccacc cgtgtagccg gcggacggca ggttttcggc gtaggccttg aaggacttgc
     1261 ccgatatcag gagctgacgc acgacgttgt tcgccgcgac cggaaaactc gacggcgttt
     1321 cgttgtcgtc attggtcagc acctgtcccg tggtcaacat gaagtaattg ccgatcgacg
     1381 gatgagtgtt ggcgtaatac tgcgtcgcca acccgtaatc gcgaatcagc gagttgaggt
     1441 agggcatctc ggaactgccg accacgctcg aggcattttg attttcctcg agcacgatca
     1501 cgacgtgtcg aaacggcgcg gcagtgggcg gcgccgccac ggccatcacg gttaaatccg
     1561 ccacgttgct gctgaccgag ccggcggcgt tcttgacggt aacctgaaag gcggctccat
     1621 tgtcggacgt cgctgtggcg ggggtcgtat ag
//
LOCUS       DASWZT010000511         4600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_33314, whole genome shotgun sequence.
ACCESSION   DASWZT010000511 DASWZT010000000
VERSION     DASWZT010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33314"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VGE92_14660"
     CDS             complement(<1..495)
                     /locus_tag="VGE92_14660"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY1315119.1"
                     /translation="MSAPLAGLHALITGAGRGIGAAITRTLADAGADVSLLGRSRPDL
                     ERLAGELPAVRSSCQAADVADGESVRAAVAAARNALGPIAILVNNAGQAHSAPLGGTS
                     DALWHSVLAVNLTGTFLCMRECLPDMVKAGFGRIVNIASTAGLVGYPYVSAYCASKHG
                     VIGLT"
     gene            complement(495..2780)
                     /locus_tag="VGE92_14665"
     CDS             complement(495..2780)
                     /locus_tag="VGE92_14665"
                     /inference="COORDINATES: protein motif:HMM:NF006101.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HEY1315120.1"
                     /translation="MKRVVFIGGGPAGLYGAILLKKALPAVKVEVHERNRPDDTFGWG
                     VVFSDQTMSGFRAADPPTHDAITQDFHHWDDIDVHLRGETIRSGGHGFCGIGRRRLLN
                     IFQERARALGVEQHFQAEVDDLAPFADADLTVASDGVNSKIRTRYAAVFQPNIAVGQC
                     RFIWLGTRQTFAAFTFAFEKTAHGWFQIHAYQFSDDLSTVIVETREETWRAHGIDTFS
                     AAQSIAFCESLFAKYLGGCPLMSNANHLRGSAWLRFNRVLCAQWHHKNLVLIGDAAHT
                     AHFGIGSGTKLAMEDAMSLVARVAGAADVPAGLITYQAERSLEALKLQSAARNRMEWF
                     ENVARYAELEPKQFAYALLTGSQRIGHANLKLRDAGFVQRYERWFAARSGLAEGERPP
                     MFLPITLAGMRLENRVVVSPMAQYMAKDGMPDDWHLMHYGARAVGGAGLLFTEMTCVS
                     PSGRITPGCTGLWNEAQRDAWRRIVDFVHAHSRAKICLQIGHSGRKGSTQLGWQRMDY
                     PLDTGNWPLLAPSALPYMAGISQIPAAMTESDMVAVRDEFVRSARLGLEADFDMLELH
                     LAHGYLLGSFLSPLTNLREDEYGGDVGARLKFPLEVFAAVRAVWPAERPLSARISACD
                     WADGGLSEADLLAIAAALKRAGADVLDVSSGQTVADQKPVYGRMWQTPFSDQIRNGIG
                     IPTIAVGNIFEADHVNSIIAAGRADLCALARPHLANPAWTLSAAAQQHYQEQAWPAPY
                     LSGKAQLERNLERAAQAVGPV"
     gene            complement(2826..4298)
                     /locus_tag="VGE92_14670"
     CDS             complement(2826..4298)
                     /locus_tag="VGE92_14670"
                     /inference="COORDINATES: protein motif:HMM:NF006179.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta
                     family protein"
                     /protein_id="HEY1315121.1"
                     /translation="MSEAKPITLTIAALGGQGGGVVADWLIDVARRSGYLAQATSVPG
                     VAQRTGATIYYLEFFPIAGIPAGQRPIFALMPHPGDVDVVIASEIMEAGRAMQRGLVT
                     ERTTLIASSHRIFSIQEKSHRGDGRADASRVRELARQHAGRYVEIDMQAVAEASGSVI
                     SAAMLGALCGSGALPFAPDHYTAAIRASGIEALSSSRAFEAARAAAGGAPQADAAPQA
                     APPRALSGGWEERIRSRVPASVQAVAREGVRRMLDYQDCAYARLYLDRLEAVIALEPS
                     RAECPLSESVARGLALWMSFEDTIRVADLKIRSARTRRVLEELRPSERQLVAVTEFMK
                     PRIEELCGTLPTRLGRWILRSKRPRRWLTRLTRGRRIETTSIGGFVQLYWLASLRRWR
                     RNTLRYADEERRIGRWLDFIGQTARQDYALALEIAECQRLVKGYGETHERGTASFNAI
                     LADLDDRPQRREAAARVRTLCAAALADDEGEALSKALAAG"
     gene            complement(4295..>4600)
                     /locus_tag="VGE92_14675"
     CDS             complement(4295..>4600)
                     /locus_tag="VGE92_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315122.1"
                     /translation="DVCTGDHSCIRLSGCPSLTIKANPDPLRRDPVAHVDNSCVGCGV
                     CGEVAHAAVLCPSFYRADVITNPGVWDRVKARIRASLIGYLQRRFDRRLARIAEESV"
BASE COUNT          820 a         1567 c         1471 g          742 t
ORIGIN      
        1 ggtcaagccg atcacgccat gtttcgacgc gcagtaggcg ctcacgtacg gatagccgac
       61 caacccggcg gtgctcgcaa tattcacgat gcgaccgaat ccggccttga ccatgtcggg
      121 caggcactcg cgcatgcaca aaaaagtgcc ggtgagattg accgccaata cggagtgcca
      181 gagcgcatcg ctcgtgccgc cgagcggtgc gctgtgcgcc tggcctgcat tgttcaccag
      241 gatcgcgatc ggtcccaagg cgttgcgcgc cgccgcgacg gccgcgcgta ccgattcgcc
      301 gtccgcgaca tcggccgcct ggcagctgct gcgcacggca ggcagctccc cggcgaggcg
      361 ttcaagatcg gggcgcgagc ggccgagcaa gctgacatcg gcgccggcat cggccagtgt
      421 gcgcgtgatc gccgcgccga tgccgcgccc tgcgcccgtg atgagcgcat gcaatccggc
      481 gagcggtgcg ctcatcagac cggacccacg gcttgtgcgg cccgctcgag attgcgttcg
      541 agttgtgcct tgccggatag atacggcgca ggccaggcct gctcctggta gtgctgctgc
      601 gccgccgcgc tcaatgtcca cgcgggattc gccaggtgcg gccgcgccag cgcacacaga
      661 tccgcgcggc ccgcggcgat gatggaattc acgtgatccg cctcgaatat attgcccacc
      721 gcgatggtcg gaatcccgat cccgttgcgt atctggtccg agaacggggt ttgccacatg
      781 cggccgtaga ccggtttttg gtctgcgacc gtctggccgc tcgacacgtc gagcacatcg
      841 gcaccggccc gtttgagcgc cgcggcaatc gccaacagat ctgcttccga gagaccgccg
      901 tcggcccagt cgcaggcgga tattcgcgcc gacagcggac gctcggccgg ccataccgcg
      961 cgtacggccg cgaaaacctc cagcggaaac ttgaggcgcg cgccgacatc gccgccgtat
     1021 tcatcctcgc gcagattggt caggggcgag aggaaactgc cgagcagata gccgtgcgcg
     1081 agatgcagct ccaacatgtc gaaatccgcc tcgagaccga gccgcgccga gcgcacgaac
     1141 tcatctcgaa ccgcgaccat gtccgactcg gtcatggcgg cggggatctg gctgatgccg
     1201 gccatgtagg gcagcgccga gggcgcgagc aacggccagt taccggtatc gagggggtaa
     1261 tccatgcgct gccaaccgag ctgcgtcgac cccttgcggc ccgaatggcc gatctgcagg
     1321 caaatcttgg cgcgcgaatg cgcatgaacg aaatcgacga tccgccgcca cgcatcgcgc
     1381 tgcgcctcgt tccacaatcc cgtacatccc ggcgtgatcc gccccgacgg ggaaacgcag
     1441 gtcatctcgg tgaacaagag ccccgcaccg cccaccgcgc gcgcgccgta gtgcatcagg
     1501 tgccagtcat cgggcatgcc gtctttggcc atgtattgcg ccatgggcga gaccaccaca
     1561 cgattctcga gccgcatgcc ggcaagcgtg atcggcaaga acatcggcgg ccgctcgccc
     1621 tcggcgagac cgctgcgcgc cgcgaaccag cgctcatagc gctgcacgaa tcctgcgtcg
     1681 cgcagcttga ggttcgcatg cccgatgcgc tggctgcccg tgagcagcgc ataggcgaac
     1741 tgcttgggtt cgagttcggc gtagcgagcg acattctcga accactccat tcgattgcgt
     1801 gccgcgctct gcagtttgag cgcctcgaga ctgcgctccg cctggtaggt gatcagtccc
     1861 gccggcacgt ccgccgcgcc cgccacgcgg gcgaccagcg acatggcgtc ttccatcgcg
     1921 agcttggtgc cggagccgat accaaaatgc gcggtatgcg ccgcatcgcc gatcaacacg
     1981 aggttcttgt ggtgccattg cgcgcatagc acgcggttga agcgcagcca ggcggatccg
     2041 cgcaggtggt tcgcgttgct catgagcggg cagccgccca aatacttcgc gaacaacgac
     2101 tcgcaaaagg cgatcgactg cgcggcgctg aacgtgtcga tgccatgggc gcgccaggtc
     2161 tcctcgcgcg tctcgacgat gacggtggag agatcgtcgc tgaactgata ggcatggatc
     2221 tggaaccacc catgcgcggt tttttcgaag gcgaatgtga aggcggcaaa tgtctgccgc
     2281 gtaccgagcc agatgaagcg gcattggccg acggcgatat tcggctggaa caccgcggca
     2341 tagcgcgtgc ggattttact gttcacgcca tcggacgcga ccgtgagatc ggcatcggcg
     2401 aacggcgcaa gatcatcgac ctccgcttga aaatgctgtt cgacgccgag cgctcgagcg
     2461 cgctcttgaa agatattgag cagccggcgc cggccgatcc cgcaaaagcc gtgaccgccc
     2521 gaacgaatcg tctcgccgcg caagtgcacg tcgatgtcgt cccagtggtg gaaatcctgc
     2581 gtgattgcat cgtgggtcgg agggtccgcc gcgcggaaac ccgacatcgt ctgatccgag
     2641 aagaccacgc cccagccgaa ggtatcgtcc ggccgattgc gttcatgcac ttcgaccttg
     2701 accgccggca gcgccttctt cagcaggatc gcgccgtaca agcctgcggg accgccaccg
     2761 atgaacacga cgcgcttcat ggcctcacaa tagatgcgga tactttagat gtcaaatata
     2821 tgcggtcaac cggccgcgag cgccttggac aacgcctctc cctcgtcatc ggcgagtgcc
     2881 gcggcgcaca gcgtacgcac cctggcggcc gcctcgcgac gctgcggacg gtcgtcgaga
     2941 tcggcaagga tggcgttgaa actcgccgtg ccgcgctcgt gggtttcgcc ataacccttc
     3001 accagccgtt ggcactcggc gatctccagc gccagcgcgt agtcctggcg cgcggtttgc
     3061 ccgatgaaat cgagccagcg gccgatgcgt cgctcctcgt ccgcgtagcg caaggtgttt
     3121 cggcgccaac gcctcaaact ggcgagccag taaagctgca cgaagccccc aatgctcgtg
     3181 gtctcgatcc gccggccgcg tgtcagacgc gtcagccagc gccgcggccg cttcgagcgc
     3241 aggatccagc ggccgaggcg cgtcggcagc gtcccacaca gctcctcgat ccgcggcttc
     3301 atgaattcgg tgaccgcaac cagttggcgc tctgacggtc gaagctcctc gagcacgcgt
     3361 cgcgtccggg cgctgcgaat cttcaaatcc gcaacgcgga tcgtatcctc gaaggacatc
     3421 cacagggcga gaccgcgcgc caccgactcg ctcaaagggc actcggcgcg tgacggttcc
     3481 aatgcgatga cggcctcgag ccggtcgaga tacaggcgcg cataggcgca gtcttggtag
     3541 tcgagcatcc ggcgcactcc ctcgcgcgcc acagcctgga ccgacgccgg cacgcggctg
     3601 cggattcgct cctcccaacc gcccgacagc gcgcgcggcg gcgcagcttg cggcgcagca
     3661 tccgcctgcg gggcgccgcc agccgcagcg cgcgccgcct cgaacgcgcg gctgctcgat
     3721 agcgcttcga tgccgctcgc acggatcgct gcggtgtagt ggtcgggcgc gaagggtagc
     3781 gcaccggaac cacacagcgc gcccagcatc gcagcgctga tgacgctgcc gctggcttct
     3841 gccacggcct gcatgtcgat ttcaacgtag cgtcccgcat gctggcgtgc gagctcgcgc
     3901 acccggctcg catcggcgcg gccgtcgccc ctgtgcgatt tctcttgtat cgagaaaatc
     3961 cgatgcgacg acgcgatgag cgtggtgcgc tcggtcacga gcccgcgctg catcgcccgc
     4021 ccggcctcca tgatttccga agcaatgacc acatccacat cgccgggatg cggcatcagc
     4081 gcaaatatcg gccgctggcc ggcgggaatg cccgcaatcg gaaagaattc caagtagtag
     4141 atggtggcgc cggttctctg cgcgaccccg ggaacggacg tcgcctgcgc cagatagccc
     4201 gatcgccggg cgacatcgat cagccaatcg gcgacgaccc cgccgccctg cccgcccaat
     4261 gcggcgatgg ttaaggtgat gggcttcgct tcgctcacac cgattcctcg gcgatgcgcg
     4321 ccaggcgccg gtcgaaacga cgctgcaggt agccgatcag cgacgctcgg atgcgggctt
     4381 tcacccgatc ccacacgccg ggattggtaa tgacgtccgc ccggtagaaa ctcggacaca
     4441 ggaccgccgc gtgcgcgact tcgccgcata cgccgcatcc gacgcagcta ttgtcgacat
     4501 gcgcgaccgg atcgcgccgc aaggggtccg gattggcctt gatggtgaga gacgggcagc
     4561 cggagaggcg aatgcacgag tgatccccgg tgcacacatc
//
LOCUS       DASWZT010000512        17188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_47731, whole genome shotgun sequence.
ACCESSION   DASWZT010000512 DASWZT010000000
VERSION     DASWZT010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 17188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17188
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47731"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..2277)
                     /locus_tag="VGE92_14680"
     CDS             complement(19..2277)
                     /locus_tag="VGE92_14680"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEY1315123.1"
                     /translation="MLGIKSTAIERPAADASFDPYVRLLRMLMPSLRGVVVHDGFSNL
                     VWASDEWDLADQPEIVKAAIANALADSAEFSGVVQTLDADSAVYTFAIRGEDIELLGV
                     VSLIARLSGSQSEARPLQTVRQLVQPALECLRRELALRSKLGSRERDLDVRERDFDLV
                     MSISSNQTTAASDADEFGLIMKTALERVNCVLAALWVPDKNIALTLTRSGQPMSAEPL
                     QRAQQHLMAWMRLQQRTIVINHISKVATDVAAPYKILACPVRHPSERVMGVLALFNPP
                     SAQDFDMHQTRLAEALAKRATSIIQAQYDSSTGLMTRQAFESQASALIGATGSAKTHI
                     ILYLDIDRLHVINETFGMHVGDDVIVNVAECMAKSLPPGALSARISGDRLAALIPNSN
                     MDEAAIIAEKIRAAAAAIAPRAGQGSFEVSVCLGVAPIGRSDNPLAHALATAEIACKA
                     AKDRGRDRVEMFQDSDHSIIRRHTDILVIGKLRDALANDNFRLDAQPILPLRGNYGRP
                     RFELLIRMLGDRGEIIPPSKFLSAAERYQLMPTVDRWVVHRACTLLGAHSASVGEDIA
                     RFAINLSGQSLQDESFLQYVIDEIKVSRLPPGVLCFELTETATIGNLAKAQGFIRKLQ
                     DLGCQFALDDFGTGVSSLAYLKDLSVNYLKIDGSFVRDAITNSRSESMIKAIAQLAKV
                     MCMETIAEYVETDLLRARMADLGVDYGQGFAMGKAQPLEVLLEELAIYEATVSNWVGT
                     PPTDPDTLPPRG"
     gene            complement(2439..2840)
                     /locus_tag="VGE92_14685"
     CDS             complement(2439..2840)
                     /locus_tag="VGE92_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315124.1"
                     /translation="MGPKMRSDLPGTFVVLDQGRGAWRLKSEAAQIEVIVRGAQAPSA
                     ALPAIVRDLDIEWRRDDVLLGLTSGEQHRFIRAQTVIVHEPLANLYATLPLATLDANA
                     RRFWSRVFRLLRIPGGRHLLGLLAGRSRAPH"
     gene            2859..4283
                     /locus_tag="VGE92_14690"
     CDS             2859..4283
                     /locus_tag="VGE92_14690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY1315125.1"
                     /translation="MDTSIKLLPGQTLDSGGRPRLDYATVTSLALPFMLNSAVQAVLN
                     ATDTWFIGRLSPAATSAIGAVYWPILVCVLLFGGFALSVQTLVAQAFGGRRYARASQA
                     TWTALWASLFTVPAFALLALTGAWIFGPFGIPRETLQLALEYWFPRMLGAPLGIALWS
                     VLGFFNGIGRPAITLWISIGVAVANALLNQLFMFDFRMGVAGSGWATNAAQSIGVIAA
                     AALFLGRATRERYRSHLTVGLHAGALLRQLKLGFPMGLLIAADILGFALFQLMQVRLG
                     TVDGASTQIVMMLTSFCYMPGVGIAMAGTTLVGQAIGAHRRDWGLQVGNGIILLTVIY
                     MGAIGVLLAAAGPWVLPWFTNRADPLAADVVARACTLLWIAAGYQLFDGLNIGCSACL
                     RGAGDVRLPAVMVLALSWLLFVPLAHSLSFAPGTGWVTWLPQFGYGAIGGWFAALSYV
                     CCLGVALLLRWRSCAWQRIALPLG"
     gene            4340..5737
                     /gene="dacB"
                     /locus_tag="VGE92_14695"
     CDS             4340..5737
                     /gene="dacB"
                     /locus_tag="VGE92_14695"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEY1315126.1"
                     /translation="MLCFTAGGAAANPAAGPPPAVAQVMAAQRLPANALSFVIVDTDS
                     GHPILSHNPDTPRSPASTIKVVTTFAALDLLGPTYTWHTRASVHGTLRAGVLDGDLIL
                     QGGGDPYMTLERWWSFARDLQAKGLKAIHGDIVIDNTAFSLPAEDPAEFDGRPNRSYN
                     VLPDALMVNFQSIEFRVLPDADARRVDVIASPTPANLEIDNKVRFAAGRCGGASGRVD
                     FKVASASWDRVVFSGALSAHCAERSFTRVLLQPASYAFGTFVELWHELGGEFAGKMRV
                     EAAPADAQALLTFDSLSLAEIVRLTNKFSSNLMARHLLLTMGEERLGGPATLEKGAAA
                     IAAWSRDRGLDLQGMDIDNGSGLSRSTHISALQMAHTLSAAYRSRYAPEFIASLPLAG
                     MDGTLRSRMKLSPAGAVRLKTGHLDGVSAVAGYVTANSGKTYVLVSLINDARADYGAG
                     DPVHAALVNWLLENL"
     gene            complement(5740..6351)
                     /locus_tag="VGE92_14700"
     CDS             complement(5740..6351)
                     /locus_tag="VGE92_14700"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY1315127.1"
                     /translation="MNKRVLRGAADTAFDRAYFRKYYFAAATRVTTAAEMRGRAQLIA
                     AILRHAAVPVRSILDAGCGTGLLRGAFAKALPRARYVGLELSPYLCARYRWTRGSVTD
                     FAPRTPSDLVICYDVLQYLNDHDAVRALANLVKLTRAALYISALTLEDWRTNCDQSRT
                     DGAVQLRPGNWYRRRLRRHFNYLGCGVWLRKEVTAILWDMERA"
     gene            complement(6344..8173)
                     /gene="glmS"
                     /locus_tag="VGE92_14705"
     CDS             complement(6344..8173)
                     /gene="glmS"
                     /locus_tag="VGE92_14705"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004637163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEY1315128.1"
                     /translation="MCGIVGVVAERNSVPILMEGLRRLEYRGYDSAGIAVLEAGRVAR
                     LRTVGKVNVLQDALAATPILGCVGIAHTRWATHGVPTTSNAHPHLSRDGVAIVHNGII
                     ENHETLRSDLKELGYVFTSETDTEVIAHRIHHHLAACSGPFEAVRRTVAELQGAYALA
                     VICEADPECIVVAREGCPVVLGLGIDENFVASDVAALLPVTRRFMFLEEGDVAEIRRK
                     SITVIDANGALAVRPIRASELPADAVERGQYRHFMLKEIHEQPRAIAQTLEERIARGK
                     LLEAAFGPAAHQAFQKVKAVHIAACGTSFHAGMVASYIIEQICRIPARVEIASEYRYR
                     DPVITADTLFVTISQSGETADTLAALRNARRAQYLSTLTICNVAESSMVRESQLVLLT
                     RAGPEIGVASTKAFTTQLTALAMLTIALAKNRHGAALERELTSQLMQLPGLVEKTLKL
                     DAAIAALAEVFADKHHALFLGRGPLHAIAMEGALKLKEISYIHAEGYAAAELKHGPLA
                     LIDEDMPVVAVAPNNELLEKLKSNLQEVRARGGKLFVFADPQARMQSAEGVTVIEMPA
                     AASALQAPIVFAVPLQLLAYHVAVLKGTDVDQPRNLAKSLTVE"
     gene            8326..8688
                     /locus_tag="VGE92_14710"
     CDS             8326..8688
                     /locus_tag="VGE92_14710"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY1315129.1"
                     /translation="MDDSSGKKFQKDLNGGLVGLVLLAILDRSTEDLYGYEIAKRLAS
                     ANEGVSIFKEGTIYPVLRALSAANLLTSRIVPSYSGPARRYYRITDQGRGVLAEWTAI
                     WAGTRRFVDRFIDTGPLP"
     gene            8685..9608
                     /locus_tag="VGE92_14715"
     CDS             8685..9608
                     /locus_tag="VGE92_14715"
                     /inference="COORDINATES: protein motif:HMM:NF025168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HEY1315130.1"
                     /translation="MNRSAPRSIVQYLSALREELAGEDTALVQDALYDAEEYLRAEVA
                     AHPDKSESDVLELIASTYGAPDEVASAYRDTEAKVKAAMTSPRKMAKTGIGAFFGVFQ
                     DPRAYTSLFFMLLSLASGIIYFTVVVTGLALSAGFAVLIIGVPFFLLFIGLTRVLALV
                     EGRLIEAMTGERMPRRPVHQSSASGFWSRVGQMLRERRTWTTLAYLVLMLPLGIAYFV
                     MAVVGLSLSLALIFAPLADVASRYQWFGIPPDVRMNPAWLGSAWALPLMVLAGVVLLT
                     LLMHLARGVGRLHAVYAKALLVPRSAPLAMM"
     gene            complement(9627..11099)
                     /locus_tag="VGE92_14720"
     CDS             complement(9627..11099)
                     /locus_tag="VGE92_14720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY1315131.1"
                     /translation="MPPPARVPICMLLLAASACVGPNFHRPPPPPVERYTVEPLPSTT
                     AAAPGPGGAAQRFLSDQAVPRDWWRLYGSADLDALVIEALRANPEVAAAQAALRQAQE
                     NTAAQRGSYFPTVLASFDANRNLDAVGVLSPNLASGAAMYNLFTPQVTVSYVPDIFGA
                     NRRQVETLAAQAEASRYQLDATYLTLTANVVSAAIQEAGLHAQIDATERVIALEREAL
                     SVLHRELELGAIAEGDVFAQDAVLAQLEASLPPLNRQLLQTRDSLAVLTGHLPSEFKA
                     PPLDLERLALPDDLPLGVASELVERRPDVRAAEAQLHAATAQVGVATANLLPQVTITG
                     AIGSSATAMSDLFKAGTGFWSIGANATQTLFAGGALIHHKRAADAALEQAAAQYRLAV
                     LTAFQNVADTLHALATDAEAMQAATRAASAAGMSLDVVRRQLELGSVSYLALVNAEQT
                     YQQAVASLLQARTNRLTDTAALFQALGGSTTPVTAQASAP"
     gene            complement(11099..14263)
                     /locus_tag="VGE92_14725"
     CDS             complement(11099..14263)
                     /locus_tag="VGE92_14725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEY1315132.1"
                     /translation="MNAIVSFALRQRVLMVVMLAMMLGAGAISFLKLNIEAYPDPVPP
                     LVDVVTQSTGQSAEEIERYITIPLEIQMAGIPNVQAIRTISLFGLSDVKVQFTYDFTY
                     YQAEQWVTNRLSQLAALPNGATPQISPTSPIGEIYRYRVVGPPNYSVTDLKTLEDWVL
                     ERRFKAVPGVIDVTGWGGKTKTYDIAIDQKKLMGYGLSVPQVLTALGNANINVGGQTV
                     NFGAQSAVVRGVGLIHSAEEIRRTLIADNQGAPVLIGDVAAVTIGNLPRLGVAGYNDL
                     DDIVQGIVLMRRGAQSVPTIKRVEAEVDKINHSGILPPGVSIQRIYDRSDLIRITTHT
                     VLHNMVAGVLLIFLLQWAFLGNLRSAFIVAMTIPFALSFAIGLMVLRGESANLLSVGA
                     IDFGLVVDATVIIVENIYRHLAEAAAHLGHGPSTLHRMRVRSGFRGKMGTISIAAAEV
                     SQSIFFAAAIIIAGFVPLFTLSGIEGHIFGPMAKTYAYAIAGGLIATFTVAPALSLVM
                     FRQVIEQRETPVVRALRRVYQPALEFVLGNRILTFAGIGLLAVLAIFAVRSLGMEFLP
                     KLEEGNLWVRATFPQSISLEDSDAYVNRMRVLMSRYPEVQTVVSQHGRPDDGTDATGF
                     FNAEFFVPLKPFETWPAGVDKEKLTETMTDELQQQFPGVEFNFSQYIQDNVEEAASGV
                     KGENSVKVYGNDLQTLEKTADDIAAVLAKVQGIEDLAVLRSLGQPEIRIDVDRVRAAR
                     YGLAPGDVNAVVQAAIGGQSAGDLYEGTSDRHFPMMVRLATPYRDNLEAIRRIPITAT
                     SGNGAPIQVPLEDVAEVRLVSGAAFIYREQQQRYVPIKFSVRGRDLGSAVLEAQRAVD
                     AKGLLPGGYRLEWVGEFGNLQEAIGRLEVAVPFSIALIVLLLYLNFGSLRNALLAASV
                     IPMALLGGIFALWVSGTAFSVSSAIGFVALFGIAAMDGILVLSYYHLSLDSGLDRASA
                     MLQTCKTQLRPVMMTCIVACVGLIPAAFSTGIGSQVQRPLALVVVGGMLLAPVLILLV
                     LPVLILQFSTAREMPPEAPREAGE"
     gene            complement(14260..15504)
                     /locus_tag="VGE92_14730"
     CDS             complement(14260..15504)
                     /locus_tag="VGE92_14730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY1315133.1"
                     /translation="MSDTPGDPAQPASGRHAIERRAWPRARQLRVAGFGAVAVLLLVV
                     CVWLGGRLFGGHPAPAAAAAAPPGTFRATAQQLKTLTVATVGLHGFVSEELTEGKIAV
                     NGDRATPLFSPFSGRVTRVIAGLGDTVKQGAPLATIEASEFVQAQNELSIAAAQVKLA
                     RINETRKHALFDAKGGSLQDWQQAQSDLTAAVAALGAVRNRLKILGKSDAQIDALESA
                     KSIDPSATLSAPIAGVVVDRQVGPGQYLQAGSGTPVFTIADPSNVWLLANVREADSGL
                     VHVGQTVEVHVLAYPERTFAARISYVAALLDPVTHRLPVRADIDNRDAALKPEMFATF
                     RILTGDATQSPAVAESAVVYEGSAAHVWVASGDGLLAYRAIRIGRSNQGLVEIVDGLK
                     PGERIVTKGGLFIDQVAVPAAP"
     gene            complement(15535..17136)
                     /locus_tag="VGE92_14735"
     CDS             complement(15535..17136)
                     /locus_tag="VGE92_14735"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1315134.1"
                     /translation="MPLWDEAARSQSGPRSIQILLSAASLLFLALMMGLGAFSMQRLN
                     DVNRVSDEIRNQWLQDIRLIGDLNNYMSDYRTGEGTHLLSNTTRELAAGEQQIADLDT
                     QVSRAQRSYEALPHENSERQLYDEFARKWTAYKIIAAQVLASSHSGAKSAAIAMYMGE
                     SRRAFDAASDTLGRLTDQTVTRAGEASGRAARAYVRDRSLIIAAMFVATCLVLATVIY
                     ITHWVSRPLLGLARGMRDLAAHDLDINIAGTGRDDEIGEMARSVAVFRDNAIALVQSQ
                     HRLVEQTAKLEETLENERRLTAEQRNFVTMTSHEFRTPLTVIDAQAQRLIKLKDRLSP
                     SDLLERAMRIRSAVNRLTVIMDSLLGASRLLDGHAVYHPADFDPTTLLREVCQLHRDT
                     TRGTDVREDYGELPAVAHGDPKLLFALFSNLLSNAIKYSAAGQPVEVVARSEAGGGIL
                     VRVSDHGIGIADRDRAHLFERYFRGANSLGVGGSGVGLHLVAIVLELHGGRIEVESRE
                     GAGSIFTVHLPIGATVESTADLGAA"
BASE COUNT         3002 a         5449 c         5554 g         3183 t
ORIGIN      
        1 atgtaggact gcgctgacct agccgcgggg cgggagcgtg tccgggtcgg tcggcggcgt
       61 gccgacccag ttgctgacgg ttgcctcgta aatggccaat tcctcgagca gaacctcgag
      121 cggctgcgcc ttgcccatcg cgaagccttg gccgtaatcg acgccgagat ccgccatgcg
      181 cgcgcgcagc agatcggtct cgacgtactc ggcgatggtc tccatgcaca tgaccttggc
      241 cagctgcgcg atggctttga tcatcgattc ggagcgcgaa ttggtgatgg catcgcgaac
      301 gaaactgccg tcgatcttca gatagttgac cgacaaatcc ttcaagtaag cgagcgaact
      361 gacgccggtg ccgaagtcat cgagggcgaa ttggcagccg agatcctgca gcttgcgtat
      421 gaagccctgc gccttcgcca aattgccgat cgtcgcggtc tccgtcagct cgaagcacag
      481 cactcccggc ggcaggcggc tgactttgat ctcatcgatc acatactgca ggaacgactc
      541 gtcctgcagc gattggccgg acaagttgat ggcgaagcgc gcgatgtcct cgccgaccga
      601 cgcgctgtgc gcgccgagca aggtgcaagc gcgatgcacg acccatcggt ccaccgtcgg
      661 catgagctgg taacgctcgg ccgcggacag gaatttgctg ggaggaatga tttctccgcg
      721 atcgccgagc atgcggatca gcaattcgaa gcgcggtcgg ccatagttgc cgcgcaacgg
      781 caggatcggc tgagcatcga gccgaaagtt gtcgttggcg agcgcgtcgc gcagcttgcc
      841 gatgaccaga atgtccgtgt ggcgcctgat gatgctgtgg tcggagtcct gaaacatttc
      901 gacgcggtcg cggccgcgat ctttggccgc tttacaggcg atttccgccg tggccaatgc
      961 atgcgcgagc ggattatcgg agcggccgat cggcgcgacg ccgagacaca ccgagacttc
     1021 gaatgacccc tggccggcgc gcggtgcaat ggcggcagcg gcggcgcgaa tcttctccgc
     1081 gatgattgcc gcctcgtcca tgttcgaatt cgggatgagc gccgccagcc ggtcgccgga
     1141 aatgcgcgcc gacagggcgc ccggcggcag cgacttggcc atgcactcgg ccacgttgac
     1201 gatgacgtcg tcgccgacgt gcatgccgaa ggtttcgttg atgacgtgca gccgatcgat
     1261 gtcgagataa agaatgatgt gcgttttcgc agagcctgtc gcgccgatca gcgccgacgc
     1321 ctggctctcg aacgcctggc gggtcatcag gccggtgctc gagtcgtact gcgcttgaat
     1381 gatgctggtc gcccgcttgg cgagcgcctc ggcgagccgc gtttggtgca tgtcgaaatc
     1441 ttgcgcactc ggtggattga acagcgcgag cacgcccatc acgcgttccg aggggtggcg
     1501 caccggacag gcgagaatct tgtacggcgc cgcgacgtcg gtcgccacct tggaaatgtg
     1561 attgatgacg atggtgcgct gctgcagccg catccaagcc atcaagtgct gttgcgcgcg
     1621 ctgcagaggc tccgccgaca tcggttggcc gctgcgcgtc aaggtcaagg cgatgttctt
     1681 gtcgggcacc cacaatgccg cgagcacgca gttcacgcgc tcgagcgcag tcttcatgat
     1741 gaggccgaat tcgtctgcat cgctcgccgc cgtcgtctga ttcgaggaga tcgacatcac
     1801 gaggtcgaag tcgcgctcac gcacgtcgag atcgcgttcc cgagaaccca acttggagcg
     1861 cagcgcgagt tcgcggcgca ggcattccaa tgccggctgc accagctggc gcacggtttg
     1921 caatgggcgc gcctcggatt gcgaaccgga caggcgcgcg atcaggctga cgacgcccag
     1981 caactcgatg tcttcgccgc ggatggcgaa ggtatagacc gcgctgtcgg catcgagcgt
     2041 ttgcacgacg ccggaaaatt cggcggagtc cgccagggca ttggcgatgg ccgctttgac
     2101 tatctcgggc tgatcggcca ggtcccactc atccgacgcc cacaccagat tcgaaaagcc
     2161 gtcgtgcacc accacgccgc gcaagctcgg catgagcatc cgcagcaaac gcacgtaggg
     2221 gtcgaaagag gcatccgcag cgggcctttc gattgccgtc gacttgattc ccagcactgc
     2281 ggccatccta gcgtcttcga tatttggaat tcacaaatca atgtgctgga aaccggcgga
     2341 ttcagctttg acgagagtcg caattcaacg taacgcccac ggggtttccc cgtctcgctt
     2401 gacaatatga gccgattctt gtgatgcaat tccgggattc agtgcggcgc ccggctgcgg
     2461 cctgccagca ggcccagcag gtgccgtccg ccggggatcc gcagcagcct gaacactctg
     2521 ctccagaagc gccgggcgtt cgcgtccaga gtggccagcg gcagggttgc gtacaggttt
     2581 gcgagcggtt catgcacgat cacggtttgc gccctgatga acctgtgctg ctcgcccgac
     2641 gtcaacccga gcagcacgtc gtccctgcgc cactcgatgt cgagatcgcg gacgatggcg
     2701 ggcaacgcgg cgctcggcgc ctgcgcgccg cgcacgatca cctcgatctg cgcggcctcg
     2761 ctcttgagcc gccacgcccc ccgcccctgg tcgaggacca caaatgtgcc gggtaaatcg
     2821 ctgcgcatct ttgggcccat tcccgcatcc tacgcgggat ggacacctcc ataaaactgc
     2881 tccccggcca gacgttggac tcgggcggac ggccgcggct cgactatgcc acggtgacct
     2941 cgctggcatt gcctttcatg ctgaacagcg cagtgcaagc cgtgctgaat gcaaccgaca
     3001 cctggtttat cggacgtctg tcgccggcgg cgacgtccgc catcggcgcg gtgtactggc
     3061 cgattctggt gtgcgtcctg ttgttcggcg gtttcgcatt atcggtgcaa accttggtgg
     3121 cgcaggcctt tggcggccgc cgctacgcac gcgcatcgca ggcgacgtgg accgccctgt
     3181 gggcgtcgct gttcacggtc cccgcgtttg cgctgctcgc cctgacgggc gcgtggatat
     3241 ttgggccgtt cggcatccct cgagaaaccc tgcagcttgc tctggaatat tggtttccgc
     3301 gaatgctggg tgcacccctc ggcatcgcct tgtggtcggt gctcggcttt ttcaatggca
     3361 ttggccgtcc ggccatcacg ctgtggatta gcatcggtgt cgcggtggcg aacgcactgc
     3421 tgaaccaact gttcatgttc gattttcgga tgggcgttgc cggctccggt tgggcaacca
     3481 acgcggcaca atcgatcggc gtgatcgccg cggcggcctt gttcttgggc cgcgccaccc
     3541 gcgagcgcta ccgctctcac ctcacggtcg gcctgcacgc cggggcgctg ctgcgtcagc
     3601 tgaaattggg ctttcccatg ggactgctga tcgccgcgga catcctcggg tttgccttgt
     3661 ttcagctgat gcaggtgcgg ctcggaacgg ttgatggtgc gtcgacacag atcgtcatga
     3721 tgctcacgtc cttttgctac atgccgggtg tcggcatcgc catggcgggc accacgctcg
     3781 tcgggcaggc gatcggagca catcggcgcg attggggttt gcaggtcggc aacggcatca
     3841 tcttgctgac cgtgatttac atgggtgcga tcggggtgtt gctcgccgcc gccgggccgt
     3901 gggtgctgcc atggtttacc aatcgcgcgg accccctagc ggcggacgtg gtggcacgcg
     3961 cgtgcacgct gctgtggatc gccgccgggt atcaactatt cgatggcttg aacatcggct
     4021 gcagcgcctg tttacgcggc gccggcgacg tgcgccttcc ggccgtcatg gttcttgcgc
     4081 tatcgtggct gttgttcgtg ccgctcgcgc atagcttgtc gtttgcgccc ggcacgggtt
     4141 gggtgacttg gttgccgcaa tttgggtacg gcgccatcgg cggctggttc gctgccttga
     4201 gctacgtctg ctgcctggga gtggcgctgt tgcttcgatg gcggtcctgc gcatggcagc
     4261 gcatcgcatt gccgctcgga tgaggcgtgc tgaattggag agcagtcggt tgagtgtatt
     4321 gcgacaaggt gcgtgcgcgt tgctttgctt cactgcgggc ggcgcagcgg caaatccggc
     4381 cgcggggccg ccgccggcgg tcgcgcaggt catggcggcg cagcggctgc cggcgaatgc
     4441 gctcagcttc gtcatcgtcg acacggattc cggacacccg atactcagtc acaatcccga
     4501 cacgccgcgc agcccggcct cgacgattaa agtggtcacg acattcgccg ccctcgacct
     4561 gcttggaccg acttacacct ggcatacccg cgcgtcggtt cacggcacgc tgcgtgcggg
     4621 agtgctcgac ggcgatttga ttttgcaggg cggcggcgat ccgtacatga cattggagcg
     4681 ctggtggagc ttcgcccgcg atctgcaagc caagggcctc aaagcgatcc acggcgatat
     4741 cgtcatcgac aacacggcgt tctccttgcc cgcggaggat cccgcagaat tcgacggccg
     4801 gccgaatcgc tcctacaacg tcctgcccga tgcgctgatg gtgaatttcc aatcgatcga
     4861 gttccgggtg ctgccggatg ccgatgcccg gcgcgtcgac gtcatcgcga gccccacgcc
     4921 tgcgaatctc gagatcgaca acaaggtgcg ctttgccgcc ggccgctgcg gcggagcatc
     4981 cggccgcgtc gatttcaagg tcgcttcggc gagctgggat cgcgtggtat tcagcggcgc
     5041 cttgtcggcg cattgcgccg aacgcagctt tacccgtgtg ctgctgcagc cggccagcta
     5101 tgccttcggc accttcgtcg agctgtggca tgaactcggc ggggagttcg ccggcaagat
     5161 gcgcgtcgag gcggcgcccg ctgatgccca agcgttgctc accttcgatt cattgagctt
     5221 ggccgagatc gtgcgcctca ccaataaatt cagcagcaat ttgatggcgc gccatcttct
     5281 gctcaccatg ggcgaggaac gtttgggcgg ccccgcgacg ctcgagaagg gcgccgcggc
     5341 catcgccgcg tggagccgcg atcgcggact cgatctgcaa gggatggaca tcgacaacgg
     5401 ttcgggactg tcgcgcagca cgcacatttc cgcgctgcaa atggcgcaca ccctgagtgc
     5461 ggcttatcgc agccgttacg cgccggaatt catcgcgtcg ctgccgctcg ccggcatgga
     5521 tggcacgttg cgatcgcgca tgaagctaag cccggccggc gcggtgcgtt tgaaaaccgg
     5581 gcacctcgac ggggtcagcg cggtcgcggg ctatgtcacc gcgaacagcg gcaagaccta
     5641 cgtgttggtg tcgttgatca atgacgcacg cgcggactac ggcgccggcg atccggtcca
     5701 tgccgccctc gtgaattggc tgctggagaa tctttagtgc tacgcccgct ccatgtccca
     5761 gagaatcgcg gtgacctcct tgcgcagcca cacgccgcaa cccagatagt tgaaatgccg
     5821 gcgtaagcgc cgccgatacc agttgccggg gcgcagctgc acggcgccgt cggtgcgcga
     5881 ttgatcgcag ttcgtccgcc agtcctcgag cgtcagcgcg gaaatgtaca gcgcggcccg
     5941 ggtgagcttg accaggttgg cgagcgcgcg cacggcgtca tggtcgttga gatactgcaa
     6001 aacgtcgtaa cagatcacga ggtcactcgg cgtgcgcggc gcaaagtcgg tcacggaccc
     6061 tcgggtccag cgatagcggg cgcatagata cgggctcaat tcgagtccga cgtagcgcgc
     6121 acgcggcagc gccttggcga aggcgccgcg cagcaatccg gtgccgcaac ccgcatcgag
     6181 gatgctgcgg accggtaccg cggcatggcg caggatcgcg gcgatcaatt gggcgcggcc
     6241 gcgcatttcg gccgcggtcg taacgcgggt cgccgcggca aaataatact tgcgaaaata
     6301 ggcgcgatcg aatgccgtgt cggctgcgcc tcgaagtacg cgcttattca accgtcaagc
     6361 tcttcgccag gtttcgcggc tgatcgacgt ccgtgccttt caggaccgcg acgtggtatg
     6421 caaggagttg cagcggaaca gcgaatacga tgggcgcttg cagcgccgag gcggccgccg
     6481 gcatttcgat caccgtgacg ccctcagcgc tctgcatgcg cgcctgcgga tccgcgaaca
     6541 cgaacaattt tccgccgcgc gcgcgcacct cctgaagatt ggatttgagc ttctccagca
     6601 actcattgtt gggtgccacc gccacgaccg gcatgtcttc gtcgatgagt gccaacgggc
     6661 catgcttgag ttccgcggca gcgtagcctt ccgcgtggat ataggagatt tccttgagtt
     6721 tcaacgcgcc ttccatggca atggcatgca gcggcccgcg gcccaggaac agtgcgtggt
     6781 gcttgtccgc aaagacttcg gcgagcgcgg cgatggcggc gtccagtttc aaagtcttct
     6841 cgaccaggcc gggcaattgc atcagttgcg aggtgagctc gcgctccaac gccgcgccgt
     6901 gccgattctt cgcgagcgca atggtcagca tggcgagcgc ggtcagctgg gtggtaaagg
     6961 ccttggtcga ggccacgccg atttcgggcc cggcgcgagt cagcagcacc agttgcgact
     7021 cgcgcaccat cgagctctcg gcgacattgc aaatggtgag cgtcgacagg tactgcgccc
     7081 gcctggcgtt gcgcagcgcc gccagcgtgt cggcggtttc tccggactgc gatatggtga
     7141 cgaacagggt atccgctgta atcaccggat ctcgatagcg gtattcgctc gcaatctcga
     7201 cgcgcgccgg gatgcggcag atctgctcga tgatgtagct cgccaccatt cccgcatgga
     7261 agctggtgcc gcaggccgca atatgcaccg ctttgacctt ctgaaatgct tggtgcgcgg
     7321 ccggtccgaa tgccgcctcc aataatttgc cgcgggcgat ccgctcctcc aaagtttgcg
     7381 caatcgcacg cggctgttca tgtatttctt tgagcatgaa gtgacggtac tggccgcgtt
     7441 cgacggcgtc cgcaggcagt tcgctggcgc gaatcggccg caccgcgagc gcaccgttgg
     7501 cgtcgatgac cgtgatcgac ttgcggcgaa tctccgcgac atcgccctct tccaagaaca
     7561 tgaagcggcg ggtgaccggc aacagcgccg ccacatctga ggcaacgaag ttttcgtcga
     7621 tgccgagtcc aagcacgacc ggacagccct cgcgggccac gacgatacac tccggatccg
     7681 cttcgcagat cacggcgagc gcgtaggccc cctgcagttc ggcgaccgtc ctgcggaccg
     7741 cctcgaatgg gccgctgcag gcagccaaat ggtgatggat gcggtgtgca atgacctccg
     7801 tatcggtctc cgaggtgaag acgtagccca gttccttcag gtcgctgcgc agcgtctcgt
     7861 gattctcgat gatgccgtta tgcacgatcg cgacaccgtc gcgcgatagg tgcggatggg
     7921 cattgctggt ggtgggaacc ccatgagtcg cccagcgggt atgcgcgatc ccgacgcagc
     7981 ccaagatcgg ggtcgcggcc agggcatcct gcaggacgtt caccttgccg acggttcgca
     8041 gtcgcgcgac gcgccccgcc tcgagcacgg cgatacccgc ggaatcgtag ccgcggtact
     8101 cgagccgccg caggccctcc atgagaatgg gaaccgagtt gcgctccgcg accacaccca
     8161 caattccaca catagcgcgc tccgttgccg gacttaacaa aaaaaggctg cctagtgtgc
     8221 ccgaccgggc tcggaaagcc actagcccgg taaggtcgcg gtcgctaagg taagctcatc
     8281 aatattggtt gctatctact actattatat atacagtact agcttatgga cgatagcagc
     8341 ggcaagaagt tccaaaagga cctgaacggg ggcctggttg gcttggtgct gttggcaatc
     8401 ctcgaccggt cgacggaaga cctttacggc tacgagatcg ccaagcgcct ggccagtgcc
     8461 aacgaagggg tctccatctt caaggaggga accatctacc cggtgctgcg cgccctgtcc
     8521 gcggccaact tgctcaccag tcgcatcgtg ccctcttatt cggggccggc ccgccgctac
     8581 taccgcatca cggaccaagg gcgcggcgtc ttggcggagt ggaccgccat ttgggcgggt
     8641 acccgccgct tcgtcgaccg cttcatcgat acaggacctc tcccatgaat cgcagtgcac
     8701 cacgctccat cgttcaatac ctcagcgccc tgcgcgaaga actcgcgggc gaggataccg
     8761 cgctggttca ggatgcctta tacgacgccg aagagtactt gcgggccgag gtcgcagcac
     8821 acccggacaa atcggaatcc gacgtattgg aactgatcgc atcgacctat ggcgcgccgg
     8881 acgaggtagc gagcgcctat cgcgacacgg aagccaaggt gaaggccgcg atgacctcgc
     8941 cgcgcaaaat ggcgaaaact gggatcggcg cattcttcgg cgtgtttcag gatccgcgcg
     9001 cttatacatc gctttttttt atgctgttat cgctggccag cgggataatc tacttcaccg
     9061 ttgtcgtgac cggcctcgcg ctgtccgcgg ggttcgcggt gctcatcatc ggagtgccgt
     9121 tcttcctgct attcatcggg ctgacccgcg tgttggcgct ggtcgaaggt cggttgatcg
     9181 aagccatgac cggtgaacgc atgccacgtc gcccggtgca tcaaagcagc gcctcgggat
     9241 tctggtcgcg ggttggtcaa atgctgagag agcggcgtac ctggacgaca ctcgcctatc
     9301 tcgtcctgat gctgccgctc gggatcgctt atttcgtcat ggcggtggtc ggcttatcgc
     9361 tgagtctcgc cttgatattt gcgccgctgg ccgatgtcgc gagtcgctac caatggttcg
     9421 gcataccccc cgacgtgcgg atgaaccccg cgtggctggg ttccgcctgg gcgctgccct
     9481 tgatggtgct ggcgggcgtc gttctgctga cactgctcat gcacctggcg cgcggcgttg
     9541 gacgcctcca cgcggtgtac gcgaaggcgc tgctggtgcc gcgctcggcg ccgctggcga
     9601 tgatgtagcg acgccgggcg ccgagctcac ggggcgctcg cctgggccgt gacgggggtg
     9661 gtcgatccgc cgagcgcctg gaacagcgcg gccgtgtcgg tcaagcgatt ggtgcgagcc
     9721 tgcagcagcg atgcgaccgc ttgttgatac gtctgctccg cattcacgag cgcgagatag
     9781 ctgaccgagc cgagttcgag ctggcggcgc acgacgtcca aactcattcc cgcggcgctc
     9841 gccgcgcgcg tcgccgcttg cattgcctcg gcatcggtgg cgagcgcatg cagtgtgtcg
     9901 gcgacgtttt ggaaggcggt cagcaccgcc aatcggtact gcgcagcggc ctgctccagt
     9961 gccgcatcgg cggcgcgctt gtgatgtata agtgcaccac cggcgaacaa ggtctgagtt
    10021 gcatttgcgc cgatgctcca gaatccggtg cccgctttga acaggtcgga catggccgtg
    10081 gcggagctgc cgatggcgcc ggtgatggtc acttgcggca aaaggttggc ggtggcgacg
    10141 cccacctggg cggtggcggc gtgcaattgc gcctcggcgg cgcgcacgtc cggacgccgt
    10201 tcgaccagtt cggacgccac tcccaggggc agatcatcgg gcaatgccaa ccgctccaaa
    10261 tcgagtggcg gcgctttgaa ctcggagggt aagtgacccg tgaggaccgc caacgaatcg
    10321 cgagtctgca gcaactggcg attcagcggc ggcaaggagg cctcgagctg tgcgagcacc
    10381 gcgtcctgcg caaatacgtc gccctcggca atcgcgccga gttccagctc gcgatgcagt
    10441 accgacagtg cctcgcgttc gagcgcaatg acgcgctcgg tagcgtcgat ctgcgcgtgc
    10501 aggccggctt cctgaatggc cgcgctgacc acattcgcgg tcagagtcaa atacgtggca
    10561 tcgagttggt agcggctcgc ttcagcttgg gcggcgagcg tttccacctg ccggcggttc
    10621 gcgccgaaga tgtccgggac gtagctcacc gtgacctgcg gcgtgaacaa attgtacatt
    10681 gcggcgcccg aggcgagatt cggactcaag acgccgacgg cgtcgaggtt gcggttcgca
    10741 tcgaagctcg ccagcacggt gggaaaataa ctgccgcgct gtgcggccgt gttttcctga
    10801 gcctgtcgca gcgccgcctg cgccgcggcg acttctggat tggcgcgcag cgcctcgatc
    10861 accagcgcat cgagatcagc cgatccgtac aaccgccacc agtcccgagg aactgcttga
    10921 tcgctgagaa agcgctgcgc ggctcctccg gggcccggcg ccgccgccgt cgtcgacggg
    10981 agcggttcca ccgtgtagcg ctcgacaggc ggcggcggcg ggcggtgaaa gttaggaccc
    11041 acgcaagcgc tcgccgccag cagcagcatg caaatcggca cccttgcagg cgggggcatc
    11101 actcgccggc ctcccgcggc gcttccggcg gcatttcccg ggcagtcgag aattgcaaaa
    11161 tgagcaccgg caggaccagc aaaatcagca ccggcgcgag cagcatcccg ccgaccacga
    11221 ccaacgccag cggccgttgc acctgcgagc cgataccggt cgaaaatgcg gcgggaatca
    11281 gtccgacgca cgcgacgatg cacgtcatca tgaccgggcg cagctgcgtt ttgcaggttt
    11341 gcagcatggc gctcgcgcga tccaatcccg agtcgaggct caaatggtag taggacagaa
    11401 ccaggatgcc gtccatggcg gcgatgccga acagcgcaac gaacccgatc gcggaggaga
    11461 cgctgaacgc ggtaccgctc acccacaagg caaagatgcc gccgagaagc gccatgggaa
    11521 tgacgctggc cgcgagcaag gcgttacgca agctgccgaa attgagatac agcaacagca
    11581 cgatcagcgc gatcgagaac ggcacggcga cctccaagcg gccgatggcc tcttgcagat
    11641 tgccgaactc gcccacccat tcgaggcggt aaccgcccgg cagcaagcct ttggcgtcga
    11701 cggcgcgctg cgcctcgagc acggcactgc ccaggtcgcg gccgcgcacg ctgaatttga
    11761 tcgggacata ccgctgctgt tgctcgcgat aaatgaacgc cgcaccggat accaagcgga
    11821 cctcggcgac atcctcgagc ggcacttgta tcggtgcgcc gttgccgctc gtagcggtga
    11881 tcggaatgcg acggatcgcc tcgaggttgt cgcggtatgg ggtcgcgagg cgcaccatca
    11941 tcgggaaatg ccgatcgctc gtcccctcgt acagatcgcc ggcgctctgg ccgccgatgg
    12001 ccgcctgtac cacggcgttg acgtcgcccg gcgcgagccc gtaacgcgct gcccgcacgc
    12061 gatcgacgtc gatgcgaatt tccggctggc cgagcgagcg cagtaccgcg agatcctcga
    12121 ttccttgcac cttggcgagc accgcggcga tgtcgtccgc ggttttttcc agcgtctgca
    12181 gatcgtttcc gtacaccttg accgaattct ctcccttgac gccggaggcc gcctcctcga
    12241 cgttgtcctg gatgtactgc gagaaattga attccacgcc cgggaactgc tgctgcagct
    12301 cgtcggtcat ggtctccgtc aatttttcct tgtcgacgcc ggccggccag gtctcgaagg
    12361 gctttaacgg cacgaagaat tcggcattga agaagcccgt ggcgtcggtc ccgtcgtccg
    12421 gccggccgtg ctgcgacacc accgtctgca cttccgggta gcggctcatc agcacgcgca
    12481 tgcgattgac gtaggcatcg ctgtcctcga gcgatattga ctgcggaaag gtggcgcgga
    12541 cccagagatt tccctcttcg agcttcggca ggaactccat tcccaatgag cgcacggcga
    12601 aaatcgccaa cactgcaagc agcccgatcc cggcgaaggt gagaatgcgg ttgcccagca
    12661 cgaactcgag cgccggctgg taaacacgcc gcaaggcacg aaccaccggg gtttcgcgct
    12721 gctcgatcac ctgccgaaac atgaccagac tcaatgccgg cgcgaccgtg aacgtggcga
    12781 tcaatccgcc ggcgattgca tacgcatagg tcttggccat cggtccaaaa atatgcccct
    12841 cgatgcccga cagcgtgaac agcggtacga agcccgcgat gatgatggcg gccgcgaaaa
    12901 aaatggattg actgacctcg gccgcggcaa tcgatatggt gcccatcttg ccgcggaagc
    12961 cgctgcgcac gcgcattcga tgcaaggtgc tcgggccgtg cccgagatgc gccgcagcct
    13021 cggcgagatg ccggtagata ttctcgacga tgatgacggt ggcatcgacg accaggccga
    13081 agtcgatggc tcccacggac aaaaggttcg ccgattcgcc gcgcaacacc atcagcccga
    13141 tggcgaacga caaggcgaat gggatggtca tcgccacgat gaaggcgctg cgcaggttgc
    13201 cgaggaacgc ccattgcagg agaaaaatca gcagcacgcc cgcgaccatg ttgtgcagca
    13261 cggtatgcgt cgttatgcgg atgaggtcgc tgcggtcgta gattcgctga atgctcacgc
    13321 ccggcggcag gatgccgcta tggttgatct tgtcgacttc ggcttcgacg cgcttgatgg
    13381 tcggcacgct ttgcgcgcca cggcgcatca gcacgatgcc ctggacgatg tcatccaagt
    13441 cgttgtatcc cgcgacgccg agccgcggca gattgccgat ggtgacggcc gccacgtcgc
    13501 cgatcagcac cggggctccc tggttgtcgg cgatcagcgt gcggcggatc tcctctgcgg
    13561 aatgaatcag cccgacgccg cgcactacgg cagactgggc gccgaaattg acggtttggc
    13621 ctccgacgtt gatattcgcg ttgccgagcg cggtcagcac ctgcggtacc gacaggccgt
    13681 agcccatcag ttttttttga tcgatcgcga tgtcgtaggt cttggtcttg ccgccccagc
    13741 cggtcacgtc gatgacgccg ggcaccgctt tgaaacgccg ctcgagtacc caatcctcga
    13801 gcgtcttgag atcggtcacc gaataattcg gcggtccgac cacgcggtag cggtaaatct
    13861 cgccgatggg actggtcggc gagatctgcg gcgtggcgcc gttcggcagc gctgcgagtt
    13921 gcgacaagcg attcgtcacc cactgctcgg cctggtagta cgtgaaatcg taggtgaact
    13981 gcactttgac gtccgacagc ccgaacagtg aaatcgtgcg aatcgcctgg acgttgggaa
    14041 tcccggccat ctggatctcg agcgggatcg tgatgtagcg ctcgatctct tcggccgatt
    14101 gtcccgtgct ctgcgtcacc acatcgacca gaggcggcac gggatcggga taggcctcga
    14161 tattcagctt gaggaaactg atggcgccgg ctcccaacat catggccagc atcacgacca
    14221 tcaacacacg ttgacgcagt gcgaacgaga cgattgcgtt catggagcgg ccggtacggc
    14281 gacctgatcg atgaacaaac cgcccttggt gacgatgcgc tcgcccggtt taaggccgtc
    14341 gacgatttcg accaggccct ggttgctgcg accgatgcgg atggcacggt aggcaagcag
    14401 cccatcgccc gacgccaccc aaacgtgggc ggcgctgccc tcgtagacca cagccgattc
    14461 cgcaaccgcc ggcgactgcg tggcatcgcc ggtcaggatg cggaacgtgg cgaacatctc
    14521 cggtttgaga gcggcatcgc gattgtcgat gtcggcacgc accggcaggc ggtgagtgac
    14581 gggatcgagc agcgcggcta catagctgat ccgtgcggcg aacgttcgct cagggtaggc
    14641 gagcacgtgc acttccaccg tttgcccgac gtgcaccagt ccggaatccg cctcgcgcac
    14701 gtttgcgagc aaccatacgt tcgaaggatc ggcgatggtg aacaccggcg tgccgctgcc
    14761 ggcctgcaga tactggccgg ggccgacttg ccggtcgacg accacgcccg cgatgggcgc
    14821 gctcagcgtg gccgaaggat cgatgctttt cgccgactcg agcgcatcga tttgcgcgtc
    14881 cgacttgccg aggatcttca agcgattgcg taccgcgccg agcgccgcca ccgccgcggt
    14941 caagtcggat tgcgcttgtt gccaatcctg caagctgccg cccttggcat cgaacaacgc
    15001 atgcttgcgc gtctcgttga tgcgggcgag cttcacctgg gccgccgcga tgctgagctc
    15061 attctgcgcc tgcacgaact ccgacgcctc gatcgtggcg agcggcgcgc cttgtttgac
    15121 ggtgtcgccc aacccggcga tgacgcgggt cacgcggccg gaaaaaggcg agaacagcgg
    15181 cgtcgcgcga tcgccgttga ctgcgatctt gccctcggtc aattcctcgc tgacaaagcc
    15241 gtggagacca accgttgcga cggtcagggt tttcagctgt tgagcggttg cacggaaagt
    15301 cccgggcggc gcggccgccg cggcagcggg cgccgggtgc ccgccgaaca gccgccctcc
    15361 gagccaaacg caaacgacga gcagcagcac tgcaacggcg ccgaacccgg cgacccgcag
    15421 ttgccgggcg cgcggccatg cgcgtcgctc gatggcgtgc cggccgctcg ccggctgcgc
    15481 gggatcgccc ggcgtatcgc tcatctcggt atccgcgggg gttgcatcga atcctcaggc
    15541 ggcgccgaga tctgccgtcg actcgacggt ggcgccgatg ggtaggtgca ccgtaaatat
    15601 cgaacccgcg ccctcgcgac tctcgacctc gatacggccg ccgtgaagct cgagcacgat
    15661 ggcgaccaag tgcagtccga cgccgctgcc gccgaccccg agcgagttgg caccccggaa
    15721 gtagcgctcg aacaaatggg cgcgatcgcg atccgcaata ccgatgccat ggtcgctgac
    15781 ccggaccagg atgccgccgc ctgcctcact tcgcgcgacg acctcgacag gttggccggc
    15841 tgcagagtac ttgatggcgt tcgacagcag attgctgaac aacgcaaaca gcagtttcgg
    15901 gtcgccgtga gcgaccgccg gtagctcgcc gtagtcctcg cgaacatcgg taccgcgggt
    15961 cgtgtcgcgg tgcagttggc acacttcgcg cagcagcgtc gtggggtcga aatcggcggg
    16021 atgatacacc gcatgaccat cgagcaagcg cgaggccccg agcaagctgt ccatgatcac
    16081 ggtgagccgg ttcaccgcgc tgcgaatccg catggcgcgc tcgagcaagt cgctcggact
    16141 caaccgatcc ttgagtttga tcagccgctg cgcctgcgcg tcgatgacgg tcagcggcgt
    16201 gcggaattcg tgcgaggtca tggtcacgaa gttgcgctgt tcggcggtca ggcgccgttc
    16261 gttttccaag gtctcctcga gcttcgcggt ctgctcgacg agccggtgct ggctctgcac
    16321 cagcgcgatg gcgttgtcgc gaaacacggc gaccgagcgc gccatctcgc cgatttcatc
    16381 gtcgcggccc gtacccgcta tgtttatatc gaggtcatgt gccgccagat cgcgcatgcc
    16441 ccgcgccagg ccgagcagcg gccgcgagac ccagtgcgtg atgtaaatga cggtcgcgag
    16501 cacgaggcag gtcgcgacga acatcgcggc gatgatcagg ctgcgatcgc gcacataggc
    16561 acgggcggcg cggccgcttg cttcgccggc tctggtcacc gtctgatcgg tgaggcggcc
    16621 cagcgtgtcg ctcgccgcat cgaaggcgcg gcgcgattcg cccatgtaca tcgcgatggc
    16681 agcggacttg gcgcccgaat gcgacgacgc cagcacttgc gcggcaatga ttttgtaggc
    16741 cgtccatttg cgcgcgaact catcgtacag ctgccgctcg gagttctcgt gcggcagcgc
    16801 ctcgtaggag cgttgtgcgc ggctcacctg cgtatccagg tcggcaatct gctgctcgcc
    16861 ggcggcgagc tcgcgcgtcg tgttggacag cagatgcgtg ccttccccgg ttcgatagtc
    16921 ggacatgtag ttgttcaggt cgccgatcaa tcgaatgtcc tgcagccatt gattgcggat
    16981 ctcatcggag actcgattga cgtcgttcag ccgctgcatg ctgaaggctc cgagccccat
    17041 catgagcgcg agaaacagca gcgaggcggc cgaaagcagg atctgaatgg agcgtggtcc
    17101 actttgcgat cgagcggctt cgtcccacag cggcatgcgc tacggctcga tcaatcggcc
    17161 cgtgaccgcc ttgacggcgg cctggacg
//
LOCUS       DASWZT010000513         1549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_38028, whole genome shotgun sequence.
ACCESSION   DASWZT010000513 DASWZT010000000
VERSION     DASWZT010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38028"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VGE92_14740"
     CDS             complement(<1..239)
                     /locus_tag="VGE92_14740"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY1315135.1"
                     /translation="MTQKASPLRVGIGGPVGSGKTALTLALCRAMRERHDIAVVTNDI
                     YTEEDAQFLVQNQALSADRILGVETGGCPHTAIRE"
     gene            complement(241..912)
                     /locus_tag="VGE92_14745"
     CDS             complement(241..912)
                     /locus_tag="VGE92_14745"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HEY1315136.1"
                     /translation="MTPLALTRLLQLSSQALPIGGYSHSQGLESAIEQRSVVDEDSVL
                     RWILDVLKFSMATFEIPRLKCLMAAWALDDLPAVESLNEDYLATRESAELRAAAVQMG
                     FSLRALLYVLPDLPAATIGALRNIQEPSLPCVWGAAATAWQIEAREAALGYLWGWAEN
                     QVLVSMKTLPLGQSAGQRVLLALGAHIAQLADEPAAESYSNYAPALAILSAQHETQYS
                     RLFRS"
     gene            complement(909..1364)
                     /gene="ureE"
                     /locus_tag="VGE92_14750"
     CDS             complement(909..1364)
                     /gene="ureE"
                     /locus_tag="VGE92_14750"
                     /inference="COORDINATES: protein motif:HMM:NF009751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HEY1315137.1"
                     /translation="MSQRQGTVRLTFDARSKSRLLLTLEGGERAALIVERGRLLRGGD
                     RVITEDGREIDIIAAEESLLEAGSDDPWLIAKAAYHLGNRHVAVQVMPNRLRFLNDHV
                     LEEMVKGLGLRVHAVVAAFEPEGGAYGHHHAHGSQLPLVQPKIHEFSVK"
BASE COUNT          280 a          525 c          470 g          274 t
ORIGIN      
        1 tcttcgcgga tggccgtgtg cgggcagccg ccggtctcca cgcccagaat acggtcggcg
       61 gacaatgcct ggttctgcac caagaactgc gcatcttcct cggtgtagat atcgttcgtg
      121 accacggcaa tgtcgtgccg ctcgcgcatg gcgcggcaca gagcgagggt gagcgcggtt
      181 tttcccgagc ccaccggacc gccaatcccc acgcgcagcg gtgatgcttt ctgtgtcatg
      241 tcaggatctg aatagcctgg aatattgagt ttcgtgctgc gccgagagta tcgcgagcgc
      301 cggcgcgtag ttcgagtacg actccgcggc cggctcgtca gcgagctgcg cgatgtgcgc
      361 tcccagcgcg agcagtacgc gctggccggc cgattgcccc aggggcagcg ttttcatcga
      421 caccaagacc tgattctccg cccaccccca gaggtagccg agggcggcct cgcgcgcctc
      481 gatctgccac gcggtcgctg ccgcgcccca tacacagggc agggagggtt cttgaatgtt
      541 gcgcagcgcg ccgatcgtcg ccgcgggcag gtcggggagc acatagagca acgcgcgcag
      601 cgagaacccc atttgcaccg ccgcggcgcg cagttcggcg gattcccgtg tggcgagata
      661 atcctcattc aaggactcga cggccgggag gtcgtcgagc gcccaggccg ccatcaagca
      721 cttgagtcgc gggatctcga acgtcgccat cgaaaacttg agtacatcca aaatccagcg
      781 caatacagaa tcctcatcca ccacgctacg ctgctcgatc gcggactcga gcccttgcga
      841 gtggctataa ccgccgatcg gcaatgcctg actgctcaat tgcagcagcc gcgtgagcgc
      901 gagtggcgtc acttgaccga aaactcgtga atcttcggct gcaccagcgg cagctggctg
      961 ccatgcgcat ggtggtggcc ataggcccca ccctcgggct cgaacgccgc gaccaccgca
     1021 tggacgcgca atcccagtcc cttcaccatc tcctcgagca catggtcatt caaaaatcgc
     1081 aaccggttcg gcatcacctg caccgccacg tggcggttac cgagatggta ggcggccttc
     1141 gcaatcagcc agggatcatc gctcccggct tcgagcagcg actcctcggc ggcgatgatg
     1201 tcgatctcgc gcccatcctc cgtgatcacg cgatcgccgc cgcgcagcaa gcggccgcgt
     1261 tcgacgatca gcgccgcgcg ttcgccgccc tcgagggtga gcaacaaccg gctcttgctg
     1321 cgcgcatcga aggtgaggcg tacggtaccc tggcgctgac tcatgatcgc ttccagtcct
     1381 aaaataagaa atagcgctgc gtcatgggca ggctcatcgc cggctgacag gtcagaagct
     1441 ccccgtccgc gcgcacttcg taggtctcgc tgtcgacctc gatatgcggc cgcgcgtcgt
     1501 tcaaaatcat gtcggacttg cgcagccgcc gcgtcgcgcg caccggctc
//
LOCUS       DASWZT010000514         1537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_38034, whole genome shotgun sequence.
ACCESSION   DASWZT010000514 DASWZT010000000
VERSION     DASWZT010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38034"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1333
                     /locus_tag="VGE92_14755"
     CDS             155..1333
                     /locus_tag="VGE92_14755"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY1315138.1"
                     /translation="MNANVDPAAAAGAPANGKRRKMLLLIALIFVVLGLLWAAYWVLV
                     LAKREQTDDAYVNGNKVVISAQISGTVIAVLADDTQLVKAGQVLVRLDPIDAETALSR
                     SASALAQSVRQVRQEKSSADEYDSVIETRKLELARAAADLAKREPLLADHAIAPEEVR
                     HARESVELARAALTQAVRQANSSHALVDGTEVEDNPTVLQAKAAYRDAWIASQRNAVV
                     APVTGYVAERSVQLGQHIQAGQALMTVIPLNSLWVDANFKEVQLRHLRIGQGALVRSD
                     LYGGTFIFHGHVQGMAAGTGAAFSLLPAQNASGNWIKVVQRVPVRIRIDDADLAKSPL
                     RVGLSATVTVDTTNRDGPVLAQSASDTPVGDTQVYSQDLDKANAEADAVVRRNLRPQH
                     "
     gene            1348..>1537
                     /locus_tag="VGE92_14760"
     CDS             1348..>1537
                     /locus_tag="VGE92_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1315139.1"
                     /translation="MATPAPSAAPFEYPPLHGGKLLFLTAAIATASFMEILDMTIVNV
                     SVPAISGSLGVSTSEGTWT"
BASE COUNT          290 a          478 c          501 g          268 t
ORIGIN      
        1 ccgcggaagc ttcgatcgaa caaggcgatg cgctgctgca gctcgacgcg gcggcgctgt
       61 cggcggatat agccctgcaa cgcgccctcg gcggaggcta cgaaaacccg cagcaattcg
      121 cgaactctca tacgacggcg acggcggcga caccatgaac gcaaatgtag atccggcggc
      181 agcggcaggc gcacctgcga atggcaagcg gcgcaagatg ctgctgctca tcgctctgat
      241 attcgtcgtc ttgggcctgt tgtgggcggc ctactgggtg ttggttttgg cgaagcgcga
      301 gcagaccgat gacgcttatg tgaacggcaa taaagtggtg atctcggcgc agatttccgg
      361 cacggtgatt gccgtgcttg cggatgacac gcagctggtc aaagccggcc aggtcttggt
      421 gcgcctcgac ccgatcgatg ccgagacggc gctgagccgc agcgcgagcg cattggctca
      481 atccgttcgg caagtgcgcc aggagaaatc ctcggcggat gaatacgact cggtgatcga
      541 gacgcggaag ctcgaattgg cgcgcgccgc agccgatctc gccaagcgcg agccgttgct
      601 ggccgaccat gccatcgcgc cggaggaagt gcgtcacgcg cgcgaaagcg tcgaattggc
      661 acgggcggcc ttgacccaag cggtgcgcca agcgaactcc tcgcacgcct tggtcgacgg
      721 caccgaagtc gaggacaatc cgacggtgct gcaggcgaag gcggcgtatc gcgacgcctg
      781 gatcgcgtca cagcgcaatg ccgtggtggc tccggtcacc ggctacgtgg ccgagcgcag
      841 cgttcagttg ggccagcata ttcaggcggg ccaagcgctc atgacggtca tcccgctcaa
      901 ttccttgtgg gtggatgcca atttcaagga ggtgcagctg cggcacctgc gcatcggaca
      961 gggggctctg gtgcgcagcg acctgtacgg cggcacattc atattccacg gccacgtgca
     1021 gggcatggcg gccggtaccg gcgcggcgtt ctccctgctg cctgctcaga atgcgtcggg
     1081 caactggatc aaagtcgtgc agcgagtccc ggtccgcatt cgaatcgacg acgctgatct
     1141 cgcgaagtcg cctctgcgtg tcggtctttc cgccaccgtc acggtggata cgacgaatcg
     1201 cgacgggccg gtgctggcgc agagcgcgag cgacacgccg gtcggtgata cccaggtgta
     1261 cagtcaggat ctcgacaaag ccaacgccga agccgatgcc gtcgtgcgtc gcaatcttcg
     1321 gccgcaacac tgatcgcgaa acctccggtg gccacacccg caccgagcgc cgctccgttc
     1381 gaatatccac cactgcatgg tggcaagctg ctattcctca cggcggcgat cgccaccgcg
     1441 tctttcatgg aaattcttga catgaccatc gtcaacgttt ccgtgcccgc gatttcagga
     1501 agtttgggcg taagcacctc ggagggcacc tggacca
//
LOCUS       DASWZT010000515         1792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_8924, whole genome shotgun sequence.
ACCESSION   DASWZT010000515 DASWZT010000000
VERSION     DASWZT010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8924"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /gene="aguA"
                     /locus_tag="VGE92_14765"
     CDS             <1..866
                     /gene="aguA"
                     /locus_tag="VGE92_14765"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR03380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047632 - agmatine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEY1315140.1"
                     /translation="VRVVEISSNDAWIRDTGPTFVIDAKGRRRGVDWTFNAWGGLHGG
                     LYFPWDRDDEVAHKVLEIEAADRYRTSFVLEGGAIHVDGQGTCLTTEECLLNPNRNPT
                     LTRADVEEQLRRYLGIRCVIWLGRGVHLDETGGHIDELACFTAPGHVALTWTSDRKDP
                     QCEISEDALRRLSKARDARGRALTIHKIHQPGPLYMTAEEAAGIDPRAGSRPRKAGDR
                     LPASYINFYIANKCVVMPLYDKRHDRAAKRALQRLFPTRKVLGVASREVLLGGGNIHC
                     ITQQVPRPAGR"
     gene            961..>1792
                     /locus_tag="VGE92_14770"
     CDS             961..>1792
                     /locus_tag="VGE92_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315141.1"
                     /translation="MHSLIDRYARKSLSALLCAVLVLAGCHHNNYNSGYGIAWVTLTD
                     EPGDFTTYTVNVDSVTLTGAANGVVTALGTVETVDFTKLGNISELWAAANVPNDTYTS
                     ATITLDYTNAVISVLVNGVSKRATVQNTAHAAVTTITVTVNLDPSNPLVITPTPASTS
                     AVPLAIDFNLAASNQVDTSAAVPVVTVNPYMTVGIKPADTKLIRVRGPLINSSVGLGT
                     YTLYVRPFYDEINSLGSLSLFSKPNTIFTINGSIYIGTAGITALSQLSAGTTMTAAYT
                     T"
BASE COUNT          372 a          573 c          512 g          335 t
ORIGIN      
        1 cagtgcgcgt cgtcgagatc agcagcaatg acgcctggat tcgcgacacc ggacccactt
       61 tcgtcatcga tgccaagggc aggcgccgcg gcgtcgattg gaccttcaac gcctggggcg
      121 gcctgcacgg cggattgtac ttcccgtggg atcgcgacga tgaggtcgcg cacaaggtgc
      181 tcgaaatcga agccgcggac cgataccgca cctctttcgt gctcgagggc ggcgcgatcc
      241 atgtggatgg ccagggtacg tgtctgacga ccgaagaatg tttgctcaat ccgaaccgca
      301 atccgacgct cactcgggcc gacgtcgagg aacagttgcg gcgctatctc ggcatccgct
      361 gcgtcatctg gctcggccgg ggcgtgcatc ttgatgaaac cggcggacac atcgatgaac
      421 tggcttgttt cacggcaccc ggacatgttg cgctcacctg gacttcggat cgaaaagacc
      481 cgcaatgcga gatttccgag gatgcattgc ggcgcttgag caaggcgcgc gacgcgcgcg
      541 gtcgagcgct cacgattcac aaaatccatc agccgggccc gctctacatg accgccgaag
      601 aggcggcggg catcgacccg cgcgccggca gccggccacg caaagcgggc gaccgcttgc
      661 cggcgtcgta catcaatttc tatatcgcga ataaatgcgt ggtcatgccg ctgtacgaca
      721 agcgccacga ccgggcggcg aagcgcgcgc tgcagcgctt attcccgacg cgcaaggtgc
      781 ttggagtcgc aagccgcgag gtgctgttgg gcgggggtaa cattcattgc atcacccagc
      841 aagtgccgcg tccggccggc cgttgacggg ccgaaatgga actttgctgc gcgcccgctc
      901 tcgaaagtca ttattctacg cagcctaaat cctcccaacc ccaccatatt ggaagctgta
      961 gtgcactcac tgattgatcg atacgcgcgc aaatcattga gcgcgttgct atgcgcggtc
     1021 ctggtcctgg ccggctgcca tcacaataac tacaacagcg gctacggcat cgcctgggtc
     1081 acgctcaccg atgagccggg cgatttcacc acttacaccg tcaacgtcga ttccgtgact
     1141 ctgaccggcg cggccaacgg cgtcgtaacc gcactaggga cggtcgaaac cgtcgatttc
     1201 accaagcttg gaaacatttc ggaattgtgg gccgcggcga atgtgccgaa cgatacctac
     1261 acctcggcga ccatcacgct cgactacacc aatgccgtca tatccgttct cgtcaacggt
     1321 gtgtcgaagc gcgccacggt ccagaatacc gcacacgcag ccgtgacgac gataacggtg
     1381 acggtgaatc tagatccgag caatcccttg gtcattacac cgacgcccgc atcgacgagc
     1441 gccgtgcccc tggccatcga cttcaatctt gccgcatcga atcaggtgga tacgagcgcc
     1501 gccgtgccgg tcgtcacggt aaatccgtac atgacggtcg gcatcaagcc agccgatacc
     1561 aagctcattc gcgtgcgcgg ccccttgatc aactcaagtg ttggcctggg cacttatacg
     1621 ctgtacgtgc gcccattcta cgatgagatc aacagcctcg gatcgctgag cctgttcagt
     1681 aagccgaaca cgatattcac catcaacggc agcatctaca tcggcaccgc cggtattacc
     1741 gccttgtcgc aattgtccgc cggaaccacc atgacggccg cgtatactac gt
//
LOCUS       DASWZT010000516        12256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_47761, whole genome shotgun sequence.
ACCESSION   DASWZT010000516 DASWZT010000000
VERSION     DASWZT010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12256
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47761"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..297
                     /locus_tag="VGE92_14775"
     CDS             88..297
                     /locus_tag="VGE92_14775"
                     /inference="COORDINATES: protein motif:HMM:NF016028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HEY1315142.1"
                     /translation="MNAEAPLEQIQTKIAFLESAVAELSDVVFRQHQDIQVLEAKLKA
                     LADRLRSVQLDDAPRTPEQERPPHY"
     gene            complement(317..1237)
                     /locus_tag="VGE92_14780"
     CDS             complement(317..1237)
                     /locus_tag="VGE92_14780"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY1315143.1"
                     /translation="MGTVAALPSERDQNFLLASADGGKFVLKIANLGDSAQVLDLQNQ
                     AMRRVAKAAADCRVPQVLRSRQGLEIERIHHACAGTDHWMRLLTWVDGTVLANAGARD
                     VAMLESIGAGIATIGAALSGFSHPAMHRALQWDLRRAGMAREHTGLLPLTRRARILRL
                     FSEWEEIDWSNLPHSVVHGDANDHNVLIGDGRMLGLLDFGDMVYSATVCDLAIVLAYA
                     MLGERQPLCAGAHIVAAYQRRHPLTEAEQRVLLPLVLSRLCMSVCYSAHNRARNPGDP
                     YQVVSEAAAWDLLDTLEREAERTPVWPIRS"
     gene            complement(1312..1944)
                     /gene="maiA"
                     /locus_tag="VGE92_14785"
     CDS             complement(1312..1944)
                     /gene="maiA"
                     /locus_tag="VGE92_14785"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEY1315144.1"
                     /translation="MILYHHAVSSASYRVRIALALKGLPFNSRLLDLRAGEQRREEFL
                     RINSQGLVPALALDDGAVLTQSLAIIEYLDETYPRPPLLPQAPLARARVRALTLAIIC
                     DVHPLNNLRVLQYLEKELRLDKTSRDTWYRHWVQVGFDALERCLVRDAATGRFCHGDA
                     PTMADVCLVPQVANARRFDVDLKSYPRIVGIDAVCREMPEFQAAAPDRQI"
     gene            2061..3914
                     /locus_tag="VGE92_14790"
     CDS             2061..3914
                     /locus_tag="VGE92_14790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY1315145.1"
                     /translation="MIRATLKHLVTPLGLLALLLAIWCVGMFGRGYWTPDEPREADIA
                     WRMSWQADRAVPLLAGEAFCEKPPLTYWVAAVPMRLFGAAAWSARLPNLLYALITALG
                     VGLLARGSCGRLAGVAAAAAVSTFLLSYQVAIWLATDAPLLAAESIALLGAYRGWYAS
                     DTRTRLRGYLLMHAALGVGFLCKSAAAWMAPCLAILALMVAEKRWRELLRWELYVGLL
                     LQAAMILTWIWFVYAGPDGVAHLKVFFWNNLVGRFTHVDAPNELQYAAAHRNTPGKYL
                     IELPLYLAPWTLLVLAALRQAWRKRNTGSDDYRAVRFALAASLPPLLLLSMAATARNI
                     YFAPALPGVALLLGWWVHELSRHEPDRWDVGALRATALLLLLAVILCAAASGLIAADA
                     WARLNSHALLVAISALGLMLAAWFAMRAWAAARNRLGRALWSLLLAYCALLVGPASQA
                     YRQVNAWQDLPSVARAIGRDATGKPLILFAADETTRAMIDMYARTHVALIEGPIDARA
                     LLRLRSAVAAAPQSLVLVQVPGRAAGLVQRLGAKPEDTESVSAWAELAQLRLLRSYSL
                     PNGRRYALLELQPKSAALSGDAPTSEQLMVERVILDDLPRPRKILRHRVLL"
     gene            complement(3813..5285)
                     /locus_tag="VGE92_14795"
     CDS             complement(3813..5285)
                     /locus_tag="VGE92_14795"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HEY1315146.1"
                     /translation="MPPLKLCLLSSEILPYAKTGGLADVAGALVRNLRLLGHEVCAFM
                     PLYASVRAAHPELQPVLEAQDIAQRIGGSEYRFSLQTAAFPGTDVPMYFVDCPQLYDR
                     PQFYTADPDEHRRFLLFTRAALEGCRRLAIAPDIFHCNDWHTAFLPLYLKTLYRSDPL
                     LAATSTVLTIHNIGYQGIMPIAAAPDLGLGAAGLKLDAADAAHGIINSLKTGIKFADL
                     VTTVSPTYAREICEEPLGMGMQATLRARANGVVGILNGVDYDDWDPGRDPHLTVHYGP
                     HDLSGKQMNKQQLIAALHLNVAAARPLIGMVTRLAEQKGIELLFDSLPGLLQERDFAV
                     AVLGSGDPQYVSFFQGLEQRFPGRVAFRSGYDEPMAHLIEAGSDIFLMPSRYEPCGLN
                     QMYSLRYGTIPVVRRTGGLADSVQHFDPATGVGTGCVFNDYDAPAARWAIGITLDWFD
                     ASASWRKLQQNAMAQDFSWTRQIVKYDALYHELLGRRRIA"
     gene            5393..6670
                     /gene="glgC"
                     /locus_tag="VGE92_14800"
     CDS             5393..6670
                     /gene="glgC"
                     /locus_tag="VGE92_14800"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF001947.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEY1315147.1"
                     /translation="MRHAARANTGRYVSRLTSGTLAVIMAGGRGERLKALTQHRCKPA
                     TPFGGKFRIIDFVLSNCVNSGIRQIAVLTQYKSQSLIQHIQRGWSYLRGELGEFVELI
                     PAQQQIGTHWYRGTADSVFQNLDFIRSHHPTHVLILAGDHIYKMDYGPMIAYHEQCEA
                     DITVGVVDVPLDRANEFGIMTTDNTNRVTKFAEKPQDPDPIIGHEHLARASMGIYVIA
                     LERLEQLLNDNALSESTNHDFGKNIIPPAIQQSRVFAYPFQDVETRAQNYWRDVGNVD
                     ALYEANMELVALNPELNIYDHQWPIWTYQSQQPPAKFVLDEEGRRGMAVNSMVAGGCI
                     ISGAYVKESLLSSDVRIEDGSIVENSVLLPSVSIGRRCKLTRCILDENCVIPDGMTIG
                     QDRGLDEKRFFVTKRGVVLVTQDMMRATEGAKQ"
     gene            complement(6688..7761)
                     /locus_tag="VGE92_14805"
     CDS             complement(6688..7761)
                     /locus_tag="VGE92_14805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY1315148.1"
                     /translation="MSRPLVVRCGAFGDMVLLTALLRVLYARFHAPADIVTTGPWSEP
                     LLRGQPGVGEIWSMRSRKAPYWLSADQRRVVALLRLRGAGPTWYCDSNDAARHLLARA
                     RVPGELIVDAKDHPLQPGEHTIEHWRRLAQIMPPALHGPVVTAGAAGAVSPGSDLQVG
                     DPQRIDLEVWLRARGLSRQPLILIQIGNKRTMRRGLRRLAPNPKFWPHERWVEVLRHV
                     SLQYPEHAILLLGTGPEYELNRQIATQAKIARLYNVADDLPIPRLVALMSRAAGLITV
                     DSGPAHAAAAVGCPMVVLFGKAQPSLYRPWGTAGAELKVLCGQVAGQPDMLGIEARSV
                     IEAWSSLKIRDVAGAGPLTYTGR"
     gene            complement(7758..9155)
                     /locus_tag="VGE92_14810"
     CDS             complement(7758..9155)
                     /locus_tag="VGE92_14810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY1315149.1"
                     /translation="MPPKFSLIEGSIPRALLAFSLPILFGNMLQSFNGSVNAIWVGKF
                     LGEASLAAVGNANVVMFLLFAVMFGFSMASTIMVAQSVGAKNIGQAKRVVGTSAVFFL
                     GLSLTMSVLGLLLAPMLVTWLGTPADALRLALVYMRITFLALPFMGGLFFLMAVLRGA
                     GDSKTPFIYLILSVALDIALNPLLIFGWGPIPRLNLAGSALATMCAQAVSFCALVLHL
                     YRQKNFLCIHRDELRLFIVDRSLLRVLVTKGIPMGLQMFVVSSSMIALISLVNRFGSQ
                     ETAAFNAAIQLWNYVQMPALAIAAAVSSMAAQNVGAGKWDRVGRIATTGVMFNLLLGG
                     SLIALIYTLNRHALGLFLPANGAALGIAAHLNRIVLWSYVFFGTSMVLYAVVRATGAV
                     MAPLVMLTIALWIVRVPFAYWMLPRLQDDAIWWSFPIASLTSMLMSIGYYRLGGWRES
                     RLGMARAQAAPAPGT"
     gene            9220..9831
                     /locus_tag="VGE92_14815"
     CDS             9220..9831
                     /locus_tag="VGE92_14815"
                     /inference="COORDINATES: protein motif:HMM:NF028177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047869 - dimethylpropiothetin
                     dethiomethylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfonioproprionate lyase family
                     protein"
                     /protein_id="HEY1315150.1"
                     /translation="MTTAVDIAVRIKRLLESLHESALAPFLAEWPESRAIRMQAAADL
                     PVLRWLDAVCRDAAAFGIELLSALCRAAPQLAWRQTYAEKDIGRAFLDNYGWCAIISG
                     DGPLASERIACGFLLLGPQVHYPSHRHPAEEMYLILSGTAAWQRGAGAWTDRAPGALI
                     HHAGDEPHAMRTSASPLLALYLWRGAHIARAAALDPGPGVRPR"
     gene            9841..11496
                     /gene="ggt"
                     /locus_tag="VGE92_14820"
     CDS             9841..11496
                     /gene="ggt"
                     /locus_tag="VGE92_14820"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY1315151.1"
                     /translation="MTRNSPRLFAAARGDRTSGWLPQSRSEVLARNGIVATSQPLAAQ
                     AGLQILKDGGNAIDAAVAAAAVLNVVEPGSAGIGGDVFVIAWLAEEKRLVALNGSGRA
                     ASGATPQHLAARGSTGEMPLRGIDAATVPGAVDAWDVLLKRAGTLTFKELLQPAADIA
                     EQGFAVSERIHDDWIDGAAVLRTDPASLKTYLVDGQAPAAYSVFRNPDLARAFHVLQA
                     EGRDGFYQGEIAAALIAKSRALGGTLTAADLKATHADWVAPISTSYRGYDICEFPPNT
                     QGFAVLEMLNILEACAPQLDIDLAALGPRSALYWHVLIEAKKLAYADLYAHNADPAFS
                     AIPVEKLISKGYAAEQCRRIDLHAASIPDAKADPTGGTVYLVTADRWGNMVSFIYSIY
                     EGFGGGLTVPGYGFVLNNRAALFSLNPRSPNVVAPGKRPFHTLLPGFIMKDGRPLTAF
                     GLMGGAQQAQGHVQVLINMIDLGANLQAAGDAARFSHAQATNTVALESSLYDLLGSQL
                     QQLGHSVVSANGKGMGGYQAVSCIAGVYRGASDHRKDGQAVGW"
     gene            11522..11980
                     /locus_tag="VGE92_14825"
     CDS             11522..11980
                     /locus_tag="VGE92_14825"
                     /inference="COORDINATES: protein motif:HMM:NF021438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HEY1315152.1"
                     /translation="MKIPTIAAALAAFALACFVPAHAGLAADLNSPIGLWRTVDDKTG
                     APRALVRIYLQDGKYFGRIEQSFTPGAETRVCAACSDARKNQPIIGLVIIRDVTLRDG
                     VYGGGDILDPDSGSVYRCKFHLENGGATLVVRGFIGFSLLGRSQTWQRQG"
     gene            complement(12013..>12256)
                     /locus_tag="VGE92_14830"
     CDS             complement(12013..>12256)
                     /locus_tag="VGE92_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315153.1"
                     /translation="LWVVRGDFLLPRKWDGMLELRRLEIRETDDARSGCLVGLYRHVG
                     DHVKIGAGYNFTNYSDTLTDMSYRSHGFFVNTIGKF"
BASE COUNT         2286 a         3971 c         3815 g         2184 t
ORIGIN      
        1 cttggcggga tttttgagcg acacgcccga gcagaaccgc gccatcaccc gcctgtggca
       61 ggcattcaaa tcggggtcga atgcaccatg aacgccgaag cgccgttgga gcaaatccaa
      121 accaagatcg ccttcctcga aagcgcggtt gccgaattga gcgacgtcgt atttcggcag
      181 catcaggaca ttcaggtcct cgaggcaaaa ctcaaggctc tcgccgaccg gctgcgcagc
      241 gtgcaactcg acgacgctcc gcgaacgccg gagcaggagc ggccgcccca ctactgaatc
      301 gaatacggcg cctgattcaa gaccgaatcg gccacacagg cgtccgctct gcctcgcgct
      361 cgagcgtatc caacaaatcc caagccgctg cctcgctcac cacctggtac ggatcgcctg
      421 gattgcgcgc ccgattgtgc gcggaatagc agacgctcat gcagaggcgg ctcagcacca
      481 acggcagcag cacccgctgc tccgcctcgg tcagaggatg gcggcgctga taggcggcga
      541 ctatgtgcgc accggcgcac agcggttgcc gttcaccgag catggcgtag gccagcacga
      601 tcgcgaggtc gcaaaccgtc gccgaataca ccatgtcgcc aaaatcgagc aggcccagca
      661 tgcgcccgtc gccgatgagc acattatgat cgttcgcatc gccgtgaacg acgctgtgcg
      721 ggaggttcga ccaatcgatc tcctcccact ccgagaacag ccgcaggatg cgtgcccgcc
      781 gcgtcagcgg cagcaacccc gtgtgctcgc gggccatgcc cgcgcggcgc agatcccact
      841 gcagcgcgcg gtgcatcgcc ggatgggaaa agccgctgag ggcagcaccg atcgttgcaa
      901 tcccggcgcc gatcgattcc agcatcgcaa catcgcgcgc cccggcattt gcaagcacgg
      961 ttccatcgac ccatgtcaag agccgcatcc aatgatccgt acctgcgcag gcatgatgaa
     1021 tgcgctcgat ttccaatccc tggcgtgaac gcagcacctg cggcacgcgg cagtccgccg
     1081 ccgctttcgc cacgcgccgc atcgcttgat tttgcaaatc caagacttgc gcggagtcgc
     1141 cgagattcgc gattttcagt acgaattttc cgccgtccgc ggacgcgagc agaaagttct
     1201 gatcacgctc gctcggtaga gcggcaaccg tgcccacgat gccgtactgc cgctgcgcgg
     1261 cgcaaagcgc ctgcgccgcc gtaaagtgcg ggatgctgtc gagcgcaatc gtcagatttg
     1321 gcgatccggt gcggccgctt gaaactcggg catttccctg cagacggcgt cgatcccgac
     1381 gatgcgcgga taggatttga ggtcgacgtc gaagcgccgc gcgttcgcca cctgcggcac
     1441 cagacagacg tccgccatgg tcggcgcatc gccgtgacag aagcgccccg tcgccgcgtc
     1501 gcgaaccagg cagcgttcga gggcgtcgaa tccgacctgc acccagtgcc ggtaccaggt
     1561 gtcacggctc gtcttgtcga gccgcaattc cttctcgaga tattgcagca cgcgcagatt
     1621 gttcaaagga tggacgtcgc agatgatcgc caacgtcagc gcacgcacgc gcgcgcgtgc
     1681 caacggcgct tgcggcagca gcggcggccg cggataagtc tcgtcgagat actcgatgat
     1741 ggccagcgat tgggtgagca ccgcaccatc gtcgagcgcc agcgcgggaa ccaggccttg
     1801 actgttgatt cgcagaaatt cctcgcgccg ttgttcgccg gcgcgcaaat ccagcagcct
     1861 ggaattgaac ggcaggccct tcagcgccaa cgcgatccgg acgcggtagg acgccgaact
     1921 caccgcatgg tgatagagga tcatccttaa gcataggcac ccacgccacg gtattgcaat
     1981 ccggcggccc gagcgcgata catctccggg acgccacttc aatgcacgct ttggttgttt
     2041 ttgagcgatg ctaggcggct atgattcgag caacgctcaa gcacctcgtc acgccgctcg
     2101 ggctgctcgc gctcctgctc gcgatttggt gcgtcggcat gttcgggcgc ggctattgga
     2161 cgccggacga accgcgcgag gccgatatcg cctggcgcat gagttggcag gcagatcgag
     2221 cggtaccgct attggccggc gaggcgtttt gcgagaagcc gccgttgacc tactgggttg
     2281 cggccgtccc gatgcgtctt ttcggcgccg cggcctggtc ggcgcgtctg cctaatctgc
     2341 tgtacgcctt gattacggca ctcggcgtcg gcttgctggc gcgcggcagc tgcggacgcc
     2401 tcgcaggcgt ggcggccgcg gcggccgtga gtaccttcct gctttcctat caagtggcca
     2461 tttggctcgc gaccgatgcg ccgctgctcg ctgcggaatc gatcgcgctc ctgggtgcct
     2521 accggggctg gtatgcatcg gacactcgca cgcgcctgcg cggatacctg ctgatgcacg
     2581 cggccttggg agtgggcttt ctgtgcaaga gcgcggccgc ctggatggcg ccgtgcctgg
     2641 ccatccttgc cctgatggtg gcggagaaac gctggcgcga attgctgcgc tgggaactgt
     2701 atgtcgggtt gttgctgcag gcggcaatga tcctcacctg gatctggttt gtatacgcgg
     2761 ggccggacgg cgtcgcgcac ttgaaggtgt ttttctggaa caacttggtg ggccgtttca
     2821 cccatgtcga tgcgcccaac gagttgcaat acgccgctgc gcatcgcaat actccgggca
     2881 agtacctgat cgaactgccc ttgtacttgg cgccctggac cttgttggtc ctcgccgcct
     2941 tgcggcaggc gtggcgcaaa cgtaacaccg gcagcgatga ttatcgcgcg gtgcgcttcg
     3001 cactcgccgc gtcgctgccg ccactcctgt tgctgtcgat ggcggctacc gcgcgcaaca
     3061 tttattttgc gccggcgctt ccgggagtcg cgctgctgct cggttggtgg gtgcacgaac
     3121 tctcgcggca tgagcctgac cgctgggacg tcggcgcgct gcgcgcaacc gcgctgctgc
     3181 tgctgttggc cgtgattctg tgcgccgcgg cttcaggtct gatcgctgcg gacgcgtggg
     3241 caaggctgaa ttcgcatgcg ctcctcgtcg cgatctcggc cttgggtctc atgctcgcag
     3301 cgtggttcgc catgcgcgcc tgggcggcgg cgcgcaatcg gctcgggcgt gcgctctggt
     3361 cgttgttgtt ggcgtattgc gcgttgctgg tgggacccgc ttcgcaggcc taccgccagg
     3421 tgaatgcctg gcaggatctg ccgtccgtcg cgcgtgccat cgggcgcgac gcaaccggca
     3481 aacctttgat tctgttcgcg gccgacgaga cgactcgggc catgatcgac atgtatgcac
     3541 gaacccacgt cgcgctgatc gaaggtccca tcgacgcgcg tgcgctgttg cgcctgcgat
     3601 ccgccgtggc cgccgccccg caaagcctgg tgctggttca agtgccgggg cgcgccgccg
     3661 gactcgtgca gcgtctcggc gcaaagcccg aggataccga gtcagtgtcc gcctgggctg
     3721 agctcgcgca gttgcggctg ctcaggagct actccctgcc gaacggccgt cgttacgcat
     3781 tgctcgaatt gcaaccgaag agcgctgcgc tgtcaggcga tgcgccgacg tccgagcagc
     3841 tcatggtaga gcgcgtcata cttgacgatc tgccgcgtcc acgaaaaatc ctgcgccatc
     3901 gcgttttgtt gtaactttcg ccagctggcc gacgcgtcga accaatcgag cgtgatgccg
     3961 atcgcccaac gcgcggcggg ggcgtcgtaa tcgttgaaca cgcatccggt gcccacgccc
     4021 gtggccgggt cgaaatgctg caccgaatcg gccaggccgc cggttctgcg cacgaccggg
     4081 atggtgccgt agcgcaggct gtacatctga ttcaggccgc agggctcata gcgcgagggc
     4141 atgaggaata tgtcgctgcc cgcctcgatc aaatgcgcca tgggctcatc gtagcctgag
     4201 cgaaacgcga cgcggcccgg gaaccgctgc tccagtccct ggaaaaagct cacgtattgt
     4261 ggatcaccgc tgccgagcac ggccactgcg aaatcccgct cttgcagcaa gcccggcaga
     4321 ctgtcgaaca gaagttcgat gcccttctgc tccgcgaggc gggtgaccat gccgatcagc
     4381 ggccgcgccg cggcgacgtt gagatgcaga gccgcaatca gttgctgttt gttcatctgc
     4441 ttgccgctca agtcgtgcgg cccgtaatgc acggtcaaat gcggatcgcg tcccggatcc
     4501 caatcgtcat agtcgacgcc attcaggatc ccgaccaccc cgttggcgcg cgcacgcagc
     4561 gtcgcctgca tgcccatgcc caacggctcc tcgcagattt cacgcgcgta ggtcgggctc
     4621 accgtggtca ctagatcggc aaattttatg ccggttttca aggagttgat gatgccgtgg
     4681 gccgcatcgg cggcatccag cttgagaccc gccgccccca gccccaagtc cggcgcggcg
     4741 gcaatcggca tgatgccctg ataaccgatg ttatggatcg tcagcaccgt gctggtcgct
     4801 gccagcaacg gatccgaccg atacagcgtc ttcaaataca gtggcaagaa tgccgtgtgc
     4861 caatcgttgc aatggaaaat atccggcgct atcgcgaggc gccggcagcc ctcgagcgct
     4921 gcgcgcgtga acagcaggaa acggcgatgt tcgtcggggt cggcggtgta gaattgcggc
     4981 cgatcgtaca gctgcgggca gtcgacgaag tacattggga cgtcggtgcc cgggaacgcg
     5041 gcagtctgca gggaaaagcg gtattcgctg ccgccgatgc gctgcgctat gtcctgtgcc
     5101 tcgagcaccg gctggagttc cgggtgggcc gcgcgtaccg acgcgtacaa gggcatgaac
     5161 gcgcacacct catgacccag cagccgcaaa ttcctcacca atgcgccggc gacgtccgcg
     5221 aggccgccgg ttttcgcgta cggcagaatc tccgaactga gcaggcacag tttcaagggc
     5281 ggcataattg acagtttagc aaacgcttct tgagcgtcgc gagaaacctg gtgcaggcag
     5341 ttgccgagtg tggcaggctg atgctactcg gggttgggag cttaggataa gtatgcgaca
     5401 cgctgctcgt gctaacaccg gacgctacgt cagccgtttg acgagcggaa cgttggccgt
     5461 gatcatggcc ggcggacgtg gcgaacgact caaggctctg actcaacacc gctgcaaacc
     5521 tgcgactccc ttcggcggca aattcaggat catcgatttc gtgctgtcga attgcgtcaa
     5581 ttccggcatc cggcaaattg ccgtgctgac ccagtacaag tcgcaatccc tgatccagca
     5641 tatccagcgc ggttggagct atctgcgcgg tgaactcggc gaattcgtgg aactgatccc
     5701 ggcgcaacag cagatcggca cccattggta tcgcggcacc gcggactcgg tgtttcagaa
     5761 tctggatttc atccgttcgc accatcccac ccacgtgctg attttggcgg gcgatcatat
     5821 atacaaaatg gattacggcc cgatgatcgc ctaccacgaa caatgcgagg cggacatcac
     5881 cgtcggagtg gtcgacgtgc cgctcgatcg cgccaacgaa ttcggcatca tgaccaccga
     5941 caacaccaac cgcgtcacga agttcgccga aaagccgcaa gatccggatc ccatcatcgg
     6001 ccacgagcat ttggcgcgtg cctccatggg catctacgtc atcgcgctgg agcgcctcga
     6061 acaattgctg aacgacaacg ccctcagcga gtccaccaat cacgatttcg gcaagaacat
     6121 catcccaccg gcgatccagc aatcgcgggt attcgcctat ccgtttcagg acgtcgagac
     6181 gcgcgcacag aattattggc gcgacgtcgg caacgtcgac gccctgtacg aagccaacat
     6241 ggaattggtc gcgctcaatc cggaactgaa catctacgat catcagtggc cgatctggac
     6301 ctatcagtcg cagcaacccc ctgccaagtt cgtgctcgac gaggagggtc ggcgcggcat
     6361 ggcggtcaac tcgatggtcg ccggcggctg catcatctcc ggcgcctacg tgaaggagtc
     6421 gctgctgtcg agcgacgtgc gcatcgagga tggcagcatt gtcgagaatt cagtgctgtt
     6481 gccgagcgtc agcatcggcc ggcgctgcaa gctcacgcgc tgcattctcg atgagaattg
     6541 cgtcattccg gacggcatga ccatcggcca ggatcgcggc ctcgatgaga agcgattctt
     6601 cgtgaccaag cgcggcgtgg tactcgtgac ccaggacatg atgcgcgcga ccgagggcgc
     6661 gaagcagtag cgcgcctcga gcgccgctca gcgtccggtg taggtcaagg gtccggcgcc
     6721 cgcgacgtcg cgaattttca agcttgacca ggcctcgatc acgctgcgcg cttcgattcc
     6781 cagcatgtcc ggctgccccg ccacctggcc gcacaggacc ttcagctcgg ctccggccgt
     6841 accccacggc cgataaagcg acggctgcgc tttgccgaac agcacgacca tcgggcagcc
     6901 gactgcggcg gccgcatgtg ccggacccga atcgacggtg atgaggccgg cggcccgcga
     6961 catcagtgcc accagccgcg gaatcggcaa atcgtcggcg acattgtaga ggcgcgcaat
     7021 tttcgcctgc gtcgcgatct gccggttgag ctcatactcg ggtccggtgc cgagcaacag
     7081 gatggcgtgc tccggatact gcagactgac gtggcgcaac acttcgaccc agcgctcgtg
     7141 cggccagaac ttggggttgg gcgcgagacg gcgcaggccg cgccgcatgg tgcgcttgtt
     7201 gccgatctga atcaggatca acggctgtcg cgacagccct cgagcccgca accacacttc
     7261 gagatcgatg cgctgcgggt cgccgacttg cagatcactt cccggcgaca ctgcgccagc
     7321 cgcaccggcc gtaaccacgg gcccgtgaag cgccggcggc atgatttgcg ccagccgccg
     7381 ccaatgctcg atcgtgtgct cgccgggctg caacggatga tccttggcat cgacgatcag
     7441 ctcgccggga acgcgcgcac gcgccagcaa gtgccgcgcc gcatcgttgc tgtcgcagta
     7501 ccaggtcgga ccggcgccgc gcaagcgcag cagcgccacg acgcgccgct gatcggcgct
     7561 cagccagtac ggcgccttgc ggctgcgcat gctccagatc tcgcctaccc cgggttgccc
     7621 gcgcagcagc ggctcgctcc acgggcccgt ggtgacgata tccgcgggcg catgaaagcg
     7681 tgcgtacagc acgcgcagca atgcagtcag cagcaccatg tcgccgaacg ctccacagcg
     7741 cacgaccagc gggcgactca tgtcccgggc gcaggcgccg cttgcgcgcg cgccattccg
     7801 agtcgactct cacgccagcc gccgagccga tagtagccga tggacatcaa catcgaggtc
     7861 aacgatgcga tcggaaagct ccaccatatc gcgtcgtctt gcaaccgcgg caacatccaa
     7921 tacgcaaacg gcacgcgcac tatccacagc gcaatggtca gcatgaccag aggcgccatc
     7981 accgctccgg tcgcccgtac gaccgcatac agcaccatcg aggtgccgaa aaagacgtac
     8041 gaccacagca cgatacgatt cagatgcgcc gcaattccca acgccgcacc gttcgccggc
     8101 aagaacagcc ccagggcgtg ccggttgagc gtatagatca gcgcaatcaa cgaaccgccc
     8161 agcagcagat tgaacatcac gccggtcgtg gcgatcctgc cgactcgatc ccatttcccg
     8221 gcgccgacgt tttgagcggc catcgaggag accgccgcgg caatcgccag agccggcatc
     8281 tgcacgtaat tccagagttg gatggccgcg ttgaatgccg cggtttcttg cgaaccgaag
     8341 cgattcacga gggagatcaa cgcgatcatg ctggaggaga ccacgaacat ctgcagcccc
     8401 atcggaattc ccttcgtgac gagcactcgc agcaacgacc gatcgacgat gaacaggcgc
     8461 agttcgtccc gatggatgca caagaaattc ttttggcgat acaaatgcag caccagggcg
     8521 cagaagctca cggcctgcgc gcacatcgtc gcgagcgctg agcccgctag attcaagcgc
     8581 gggatcgggc cccagccgaa gatcagcagc ggattgagcg cgatgtcgag cgccaccgac
     8641 aggatcaagt agatgaacgg cgtcttcgaa tcaccggcgc cgcgcagtac cgccatcaga
     8701 aagaaaagcc cacccatgaa gggcagtgcc aggaacgtga tccgcatgta aacgagcgcc
     8761 agccgcagcg cgtccgccgg cgtacccagc cacgtgacca gcattggcgc gagcagcaac
     8821 ccgagcacgc tcatggttaa cgacagtccc aggaagaaaa ccgcactggt gccgacgacg
     8881 cgtttcgctt ggccgatgtt tttggcgccc accgattgcg cgaccatgat ggtcgaggcc
     8941 attgaaaagc cgaacatgac ggcgaacaac agaaacatca cgacattggc attgccgacc
     9001 gccgcgaggg acgcttcgcc gaggaatttg ccgacccaga tcgcgttcac ggagccgttg
     9061 aacgattgca gcatgttgcc gaacaggatg gggagcgaaa aagccagcaa cgcgcgcgga
     9121 atcgatcctt cgatcaacga aaatttgggg ggcacgaacg ctccgggcga gaagacggcc
     9181 acgccagtat aattgatccc gatacgctca ggaggcgtga tgacgacggc cgtagatatc
     9241 gccgtgcgga tcaagcggct gctggaatct ctgcacgaat ctgcgctcgc tccgtttctg
     9301 gcggagtggc ccgagagccg cgcaattcgc atgcaggctg ccgcggattt gccggtgttg
     9361 cgctggcttg acgcggtctg tcgtgacgct gcggccttcg gtattgaatt gctgagcgct
     9421 ctgtgtcgtg ccgccccgca gctcgcttgg cgccaaacct atgcggaaaa ggacatcggg
     9481 cgcgcgtttc tcgacaatta cggatggtgc gcgatcatca gcggggatgg tcccttggcg
     9541 agcgagcgga tcgcctgcgg ttttctgctg ttgggcccgc aggtgcacta tccgagccac
     9601 cgccacccgg cggaggaaat gtatttgatc ttgagcggca cggccgcctg gcaacgaggc
     9661 gccggcgctt ggaccgatcg agcgccgggc gcgctcattc atcatgccgg cgacgaaccc
     9721 cacgccatgc gcaccagcgc gagtccactg ctcgcgctgt acctctggcg tggcgctcat
     9781 attgcgcgcg ccgcggcgct cgacccgggc ccgggcgtcc gacccaggta gactatcctc
     9841 atgactcgca attccccgcg cctgtttgcg gcggctcgcg gcgaccgcac cagcggctgg
     9901 ctgccgcagt cgcgttccga agtgttggcg cgcaacggca tcgtggcgac cagccagccg
     9961 cttgccgccc aggcgggcct gcagatactc aaagatggcg gcaacgcgat cgatgcggcc
    10021 gtggccgccg cagccgtatt gaacgtcgtc gagcccggca gtgccggcat cggcggcgat
    10081 gttttcgtca tcgcgtggtt ggccgaagag aagcggctgg tcgcgctcaa cggcagcggt
    10141 cgcgctgcct cgggagcgac gccgcagcat ctcgccgcac gcggctcgac cggtgagatg
    10201 ccgctgcgtg gaatcgacgc tgccaccgtt ccgggtgccg tggatgcctg ggacgtttta
    10261 ttgaagcgcg ccggaacctt gacgttcaag gagctgttgc agccggcggc cgacattgcc
    10321 gaacaggggt tcgcggtcag cgaacgtatc cacgatgatt ggatcgacgg tgcggcggtg
    10381 ctgcgcacgg atcccgcttc cctcaagacc tacttggtgg acggccaggc gccggccgcg
    10441 tatagcgttt tccgcaaccc cgatctggcg cgtgcgtttc acgtcctgca ggccgagggc
    10501 cgcgacgggt tctatcaagg cgaaatcgcg gcggccttga tcgcgaaatc ccgcgcgctg
    10561 ggcggcactc taacggcggc ggatcttaaa gccacgcacg cggattgggt ggcgccgatc
    10621 agcaccagct atcgcggcta tgacatctgc gaattcccgc ccaacacgca aggattcgcg
    10681 gtgttggaaa tgctgaacat actcgaggcc tgcgcgccgc aattggacat cgacctcgcg
    10741 gcactcggac cccggtccgc tctttactgg cacgtgctga tcgaggccaa gaagctcgcg
    10801 tacgccgact tgtacgccca caacgccgac cccgcgttca gcgctatccc ggtggagaaa
    10861 ctcatttcca agggttatgc cgccgagcag tgccggcgca tcgatctgca cgccgcgagc
    10921 atccccgacg ccaaggccga ccccacgggc ggcacggtct atctggtgac tgccgatcgc
    10981 tggggcaaca tggtgtcgtt catctatagc atctacgaag ggtttggcgg cggcctcacg
    11041 gtgccgggct atggcttcgt gctgaataat cgcgccgcat tgttctcttt gaacccgcgc
    11101 agtccgaacg tcgtcgctcc cggcaagcgc ccgttccaca cgctgctccc gggattcatc
    11161 atgaaagacg gccggccgct gacggcgttt ggtctcatgg gcggcgccca gcaggcgcaa
    11221 ggccatgtgc aggtgttgat caacatgatc gatctcggtg cgaacttgca ggcggccggc
    11281 gacgcggcgc gcttctcgca cgctcaagcc accaacacgg ttgccctcga gtcgagtctc
    11341 tacgatctgc tgggttctca actgcaacag ctgggacata gcgtggtctc ggccaacggc
    11401 aagggcatgg gtggctatca agcggtgtcg tgcatcgccg gcgtgtatcg cggcgcgtcg
    11461 gatcatcgca aagacggaca ggccgtcggc tggtagtgat cgctcgcatc gaggtaccta
    11521 catgaaaata ccgaccattg ccgccgcact cgccgcgttc gccctggcat gcttcgtccc
    11581 ggcgcacgcc ggcttggccg ccgatctgaa ctcgcccatc ggcctctgga gaacggtcga
    11641 cgataaaacc ggcgccccgc gcgccctggt gcggatctat cttcaagatg gaaaatattt
    11701 cggacgcatc gaacagagct tcacgccggg cgcggaaacg cgcgtctgcg cggcctgcag
    11761 cgatgcacgc aagaaccagc ccatcatcgg tctcgtgatc attcgcgacg tgaccctgcg
    11821 cgacggcgta tacggcggcg gcgatattct cgatccggac tcaggttcgg tctatcgctg
    11881 caaatttcac ctggagaacg gcggtgccac gctcgtggta cgcggcttta tcggcttctc
    11941 tctgctcggc cgctcgcaga cttggcaacg tcaaggctag cctcgcggcg ccgtcgccgg
    12001 cgtaccggac ctctagaact tgccgatggt gttgacgaaa aagccatggc tgcggtagct
    12061 catatccgtc aacgtgtcgg agtaattggt gaaattgtag ccggcaccga ttttgacatg
    12121 atcgcccacg tggcggtaaa gcccgaccag acagcccgag cgcgcatcgt cggtttcgcg
    12181 gatctcgagt cgccgcagct ccaacatccc gtcccatttt cgcggcaata gaaaatcccc
    12241 gcgcaccacc cacaac
//
LOCUS       DASWZT010000517         3288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_62131, whole genome shotgun sequence.
ACCESSION   DASWZT010000517 DASWZT010000000
VERSION     DASWZT010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62131"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2303)
                     /locus_tag="VGE92_14835"
     CDS             complement(<1..2303)
                     /locus_tag="VGE92_14835"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1315154.1"
                     /translation="MVRTLVNLCVRHYGAVAALSALALLLGCWGAVRAPLDVFPEFVP
                     SQVDIQTEAPGFAPAQVEELVTRQVENAVNGASGLATLRSESIPGLSVVTITFADGFD
                     VHVARQGISERLSELGSSLPAGVGTPKLSPLVSSTMDLLKIGLTSDKVDAYGLRDTAD
                     SIIKPRLLAVPGVAHVIVFGGAVRQIQIQPDMVKLASFNVTLAQLGAAARAALPLVGA
                     GFIDLPAQRVLIESPTPHPDVETIADAVVTQRGGSPILLRDLAQVTIAPALKSGDALI
                     MGKPGVMLSLASQYGANTLNVTLAVEKAIADLAPSLQSRGITLYPRLHRPADFIERAL
                     GNLKNSLAIAAILILAVLYLFLRDVRAALIAFTAIPLSLLAAVAILERIGFTLNTMTL
                     GGFAVALGVLVDDAIIGLENILRRLRENGTLANPRPRLDVIRDASLEVREPVIYATAV
                     AIVVFLPELFTSSVQGHFVAPLALAFIFAVSASLLVAMTSTPALCALLLRHRDTRPQA
                     RWLVRLKAWQSSAVYGISKHFRMVAAVLVCLVIAALAALPFLGGTFMPEFREGHFVIQ
                     ATSAVPGTSLESMVTIGKRISAEILALPYVKSIEQQVGRAEQGEDTWGPHRSEFHVGL
                     ADDAKVDQHAAQEQLRKILEGYPGLQSEVVTFLGDRISESLSGETAQVAIKVVGEDLQ
                     ALDATGDKIVGVLNKLPGVVDLQFKKQSGTPAIAIELIPQALAATGLNAEQVLNTLES
                     AYAGARVGQTFSGIRTVDVVLLLPDTLR"
     gene            complement(2307..>3288)
                     /locus_tag="VGE92_14840"
     CDS             complement(2307..>3288)
                     /locus_tag="VGE92_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315155.1"
                     /translation="REAAAVAFAVFALGGCHDSAAPVEPKAAVAAKSPADNPPAGEVD
                     KKSLAESVLTPEQIGKLGLETATLKPIEFADEAAGYGSVIPHEGIAQAAAELATAEAT
                     ERQSRAALQRTRRLSGTAGALSADAEEISARQAAVDAAALTLARQRLAATFGQTPPWS
                     SNPALLSSLAGGSTQLVRVTFPLGALSAAAPKSLRAARIGATSERSWTMSTIWAAPAD
                     ANVPGRSFFALLRDSGAGEGERIVVWAPLGEPQAGVVIPRAAVVISDGKYWCYLEKKP
                     GTFARTQIETRKPYSDGYFVNAGIKAGDTVVTQGAAQLLAQESNSGADAD"
BASE COUNT          597 a         1101 c         1034 g          556 t
ORIGIN      
        1 cgcagtgtgt ccggcagcag cagcaccacg tccaccgtgc gaatcccgct gaaagtctgg
       61 ccgacccgtg caccggcata ggccgactcc agcgtgttga gtacttgctc ggcgttcaag
      121 cccgttgctg ccaacgcctg cggtatcaat tcgatcgcaa tcgccggcgt gccgctttgc
      181 ttcttgaatt gcaggtccac gacccctggc agcttgttga ggacaccgac aatcttgtcg
      241 ccggtggcat ccaatgcctg cagatcctcg cccaccacct tgattgcgac ctgcgcggtt
      301 tcgccgctca gactttcgct gatccgatca cccaagaatg tgacgacctc gctctgcaag
      361 ccaggatagc cctccaatat cttgcgcaac tgctcctgtg ccgcgtgctg gtccactttg
      421 gcatcgtccg ccaatcccac gtgaaactcg ctgcggtgcg gcccccaggt atcctcgccc
      481 tgctctgccc gtccgacctg ctgttcgatg ctcttcacgt acggcagcgc gagaatttcg
      541 gcgctgattc gcttgccaat cgtcaccatg gactcgagcg aggtgccggg cacggcactg
      601 gtggcctgaa tgacgaagtg tccctcgcga aactccggca tgaacgtgcc gccgaggaac
      661 ggcagcgcag cgagcgccgc gatgaccagg caaacgagca cggcggcgac catgcgaaag
      721 tgtttgctaa tgccatacac cgccgaactc tgccaggcct tcaggcgtac cagccagcgg
      781 gcctgcgggc gggtgtcgcg atgtcgcagc agcagcgcgc acagagcggg ggtcgaggtc
      841 atcgccacca acagcgaggc gctgaccgca aaaataaacg ccaaggccag gggggcgaca
      901 aaatgaccct gcacgctcga ggtgaataat tccgggagaa acaccacgat ggcgaccgcc
      961 gtcgcgtaga tcaccggttc gcgcacctcc agggacgcgt cgcgaatcac atcgagccgc
     1021 ggccgtgggt tggcgagcgt cccgttctcg cgcaggcgcc gcaagatatt ctcgaggccg
     1081 atgatcgcat cgtccacgag tacgccgagc gcaaccgcga acccgcccaa ggtcatggta
     1141 ttcagcgtga acccgatgcg ctcgagaatg gcgacggcgg ccagcagcga caatggaatg
     1201 gcggtgaagg cgatgagtgc ggcgcgcaca tcgcgtagga acagatacag gacggcgagt
     1261 atgagaatgg cggcaatggc cagcgagttt ttgagattgc cgagcgcgcg ctcgatgaag
     1321 tcggcaggac gatgcaatcg gggatagagg gtgatacctc tcgactgcaa cgagggagcg
     1381 agatccgcga ttgctttctc cacggcgaga gtgacgttta acgtattggc gccgtactgg
     1441 ctcgccaggg acagcatcac acccggcttg cccatgatca atgcatcgcc cgacttgagc
     1501 gccggggcga tcgtcacctg ggcgaggtcg cgcagcagaa tcggcgagcc gccgcgctgc
     1561 gtcaccaccg catcggcgat cgtctcgaca tccgggtgcg gcgtcggcga ctcgatcagc
     1621 acccgctgcg ccggcaagtc gatgaacccc gcgccgacca acggcaaggc ggcgcgcgcc
     1681 gccgcgccga gttgcgccag ggtgacgttg aaacttgcca gcttgaccat gtcgggttga
     1741 atctgaatct ggcgcaccgc tccgccgaac acgatgacgt gggccacgcc gggcacggcc
     1801 aacaaccgcg gcttgatgat cgagtctgcc gtatctcgca acccgtacgc atcgaccttg
     1861 tccgaggtga gaccaatctt cagcagatcc atggtgctcg acaccagcgg cgagagcttc
     1921 ggcgtaccta cgcccgccgg caggctcgag cccaattcgg acaaccgctc cgaaattccc
     1981 tgtcgggcga cgtgcacgtc gaatccgtcg gcgaacgtga tcgtgactac cgagagtccg
     2041 gggatcgact cggaacgcag ggtcgcaagc cccgaggcgc cgttgacggc gttttcgact
     2101 tgcctcgtaa ccagctcttc gacttgcgcc ggcgcgaagc cgggcgcttc ggtttgaatg
     2161 tcgacttgcg acggaacgaa ttcggggaac acatcgagcg gcgcgcgcac ggcgccccaa
     2221 cagccgagca gcaatgccaa cgcgctgagg gcggcgaccg cgccgtagtg gcgcacgcaa
     2281 agattgacca gcgtgcgcac catgaatcag tccgcatccg cgccggagtt cgattcctgc
     2341 gccaacagct gtgcggcgcc ttgggtcacc accgtgtcgc cggccttgat gcctgcgttc
     2401 acgaagtatc catccgagta gggcttgcgg gtctcgatct gcgttctcgc gaacgtcccc
     2461 ggcttcttct cgaggtagca ccagtatttg ccgtcgctga tgaccaccgc agcgcgcggg
     2521 atcactacgc ccgcttgcgg ttcgccgagc ggggcccaaa cgacgatccg ctcgccctcg
     2581 cctgccccgc tatcgcgcaa caaggcgaag aagctgcgcc cgggaacgtt ggcgtcggcc
     2641 ggcgcggccc agatcgtgct catcgtccaa ctgcgctcgc tggtcgcgcc gattcgcgcg
     2701 gcgcgcaggc tcttcggcgc ggctgccgat aacgccccga gcgggaacgt gacccgcacc
     2761 aactgcgtgc tgccgccggc gagcgaactc agcaacgcgg ggttactgct ccaaggaggc
     2821 gtctggccaa aggttgccgc gagccgctgc ctggccaagg tcaatgccgc cgcatcgacc
     2881 gcggcctgac gcgcgctaat ttcctccgca tcggcggaca gcgcgcccgc ggtaccggac
     2941 aagcgccgag ttctctgcag cgccgcgcgg ctttgccttt cggtggcctc ggcggtcgcg
     3001 agttccgcgg ccgcttgggc aatcccttca tgcggaatca cgctgccata gccggctgcc
     3061 tcgtcggcga actcgatcgg cttcaaggtc gcggtttcca agccgagttt cccgatctgt
     3121 tccggcgtca ggacactctc ggcgagcgac tttttatcga cttcgccggc tggcggattg
     3181 tcggcgggag attttgccgc aacggctgct ttgggctcca cgggcgccgc cgagtcgtgg
     3241 cagccgccca aagcgaacac cgcaaacgcc acggccgcgg cctcccgg
//
LOCUS       DASWZT010000518         2510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_23738, whole genome shotgun sequence.
ACCESSION   DASWZT010000518 DASWZT010000000
VERSION     DASWZT010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23738"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VGE92_14845"
     CDS             <1..301
                     /locus_tag="VGE92_14845"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY1315156.1"
                     /translation="VALAKELPCIGEVRGLGAMVAFELVKDLKSKEPDPELTAAILTQ
                     AETRGLILLSCGTSVNVVRLLAPLTIPETVLNEGLELLAAAVRAAYGQESKRAVA"
     gene            complement(312..>2510)
                     /locus_tag="VGE92_14850"
     CDS             complement(312..>2510)
                     /locus_tag="VGE92_14850"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1315157.1"
                     /translation="ESTGGSGTTGIYIDDVPIQVRQLGFNSDDALLKVFDLERIEVLR
                     GPQGTLFGAGSEGGTVRYITTPPSLTKDSIYAKAETSYTEGGSPSYEAGVAAGGPLID
                     GTLGVRASAWYRRDGGWIDRLDPVTFQDLDKNANYDETTVLHVAALWQPNESLTVAPS
                     IFYQNRQRNDVTIYWPTISNPSENRYFSLDPTARPEPDKLYLPSVTVTADFGPARLIS
                     TSAYFHRDEISGYDGTLYNLGYYQTLGTVDFPLLDGSGVHLPAGLTDYRAPASVTNQQ
                     RNFTQEFRLQSTDPTARFGWTTGVFYSLNRQFSLEEIHDPMVDQFFLQELGETASQAF
                     SDTVDVNPNGSSYLPMGDSYFNRLLSHDRQIAGFGELNFKITDALKLTGGVRVSKTSF
                     SIESLSGGPQNSGPRPGANSNSQIPVTPKASLQWQVDPNNMYYVTYAKGFRSGGGNPT
                     IPYDPTYQQTDVGCTQDFLNFGIPHAPATYKSDSVQSFEVGAKNNIDNRVRLASSVYY
                     ILWNNIQANVVPPICQIQWTDNLGNATVKGFDVQADVEVTSAVSMNVSFGVTDARYTK
                     NAYPSGANTTGPNPPLPLVYAGDAVAGPNGIGTGYSIPPYQGSVGLEYKFKVAAQEAF
                     LRADYEYKAADKWKDAAEDPRTSSYDPTSFPLGAQKFASLRAGTNINGWDLSMFCDNV
                     TDSHTILNFNHQTNSYDTSPPSIAGLLASPAYRYISYRPRTFGITVTFRH"
BASE COUNT          446 a          714 c          834 g          516 t
ORIGIN      
        1 tgtggcgctc gccaaggagc tgccgtgcat cggcgaggtg cgcggtctcg gggcgatggt
       61 ggccttcgaa ctcgtcaagg acctgaaatc caaagaaccc gatcctgagt tgacggcggc
      121 gatcctgacg caggccgaga ctcgcggcct gatactcctg tcctgcggta cctcggtgaa
      181 cgtggtgcgg ttgctcgcgc cgttgacgat tcccgagacg gtgctcaatg aggggctcga
      241 actcctggcc gctgccgtgc gcgcggcgta cggccaggaa tcgaagcgag cggtcgcgta
      301 acctttccgc gtcagtggcg gaaggtgacg gtgatgccga aagtccgcgg tcgataggaa
      361 atgtagcggt acgcgggact tgcgagcaga ccggcaatcg agggcggact ggtgtcgtaa
      421 gagttcgtct ggtgattgaa gttcaagatc gtatgcgagt cggtgacgtt atcgcagaac
      481 atcgataagt cccaaccgtt gatattcgtg ccggcgcgca gcgatgcgaa cttttgtgcg
      541 cccaagggga aggaggtcgg atcgtaactg gaggtgcgcg gatcttcggc ggcatccttc
      601 catttgtccg ccgccttgta ctcgtagtcc gcgcgcagga atgcctcttg cgcggccact
      661 ttgaatttgt attcgagccc cacggatccc tggtagggcg gaatcgagta gccggtgccg
      721 atgccattcg ggcccgcgac cgcgtcgccc gcgtagacca gcggcaacgg cggattcggc
      781 ccggtggtgt tcgcgccgct cgggtacgcg ttcttggtgt agcgtgcgtc ggtgactccg
      841 aagctcacat tcatgctgac ggcgctggtg acttcgacat ccgcctgcac gtcgaagccc
      901 ttcaccgtgg cgttgcccag attatcggtc cactggatct ggcagatggg cggcaccaca
      961 ttggcttgga tgttgttcca gagaatgtag tacacgctgc tcgccaaccg gacgcgattg
     1021 tcgatgttgt tcttggcgcc cacttcgaaa ctttgcaccg agtcggactt gtaggtggcc
     1081 ggcgcatggg gaattccgaa attgaggaaa tcctgagtac agccgacgtc ggtctgctgg
     1141 tatgtggggt cgtacggtat ggtcgggttg ccgccgccgc tgcgaaagcc cttggcgtag
     1201 gtaacgtagt acatgttgtt cgggtccacc tgccactgca agctcgcctt cggcgtgacc
     1261 ggtatctggc tgttcgagtt cgcgcccggg cgcggcccac tgttctgcgg tccgcccgac
     1321 aggctctcga tcgagaaact cgtcttcgag acgcgtactc cgccggtgag tttcagggcg
     1381 tccgtgattt tgaaattcaa ttcaccgaaa ccggcgatct gccggtcgtg gctcaacagg
     1441 cggttgaaat acgaatcgcc catcggcaga taggaactgc cattcgggtt cacatcgacg
     1501 gtgtcagaaa aggcttggga ggcggtctcg cccaactcct gaaggaaaaa ctgatccacc
     1561 atcggatcgt ggatttcctc gaggctgaat tggcggttga gcgaatagaa cacgcccgtc
     1621 gtccagccga agcgggcggt cgggtcggtc gactgcagcc ggaattcctg ggtgaaattg
     1681 cgctgctggt tggtgaccga ggcgggcgcg cgataatccg tcaagcccgc gggcagatgg
     1741 acacccgatc cgtcgagcag cgggaaatcg accgtgccga gtgtttgata gtagccgagg
     1801 ttgtacagcg tgccgtcgta gcccgagatc tcgtcgcggt gaaagtacgc gctcgttgaa
     1861 atcagccgcg ccgggccgaa atccgccgtg acggtgaccg acgggagata cagcttgtcc
     1921 ggttccgggc gggccgtcgg atccaagctg aagtagcggt tctcggaggg attcgagatg
     1981 gtgggccagt agatggtgac atcgttgcgc tggcggttct gatagaaaat gctgggagcg
     2041 acggtcagcg attcgttcgg ctgccacagc gccgcgacgt gcagcaccgt ggtttcatcg
     2101 taattcgcat tcttgtccaa atcctggaac gtgacgggat cgaggcgatc gatccagccg
     2161 ccgtcgcgcc ggtaccaggc gctggcgcgt acaccgagcg tgccgtcgat gagcggaccg
     2221 ccggccgcga cccccgcctc atagctcggg ctgccgcctt cggtgtagga ggtctccgcc
     2281 ttggcgtaga tgctgtcctt ggtgaggctc ggcggtgtcg tgatgtagcg taccgtaccg
     2341 ccctcggagc cggcaccgaa caacgttccc tgcgggccgc gcagcacctc gatgcgttcg
     2401 aggtcgaaca ccttcaacag cgcatcgtcc gagttgaagc cgagttggcg cacttgaatc
     2461 ggcacatcat cgatgtagat tcccgtagtg cccgagccgc cggtcgattc
//
LOCUS       DASWZT010000519         2330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_81729, whole genome shotgun sequence.
ACCESSION   DASWZT010000519 DASWZT010000000
VERSION     DASWZT010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81729"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VGE92_14855"
     CDS             <1..516
                     /locus_tag="VGE92_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1315158.1"
                     /translation="VLGVLYESYIHPITILSTLPSAGVGALLALLLFHNELTVVAIIG
                     IVLLIGIVQKNAIMMIDFALDAQRKEHKSAFDAIHEACLLRFRPILMTTMAAMLGAMP
                     LAFGHGVGSELRRPLGITIIGGLIFSQMLTLYTTPVIYLFFDGLAERFARWRAAQSGR
                     STDTAVRPSAT"
     gene            513..>2330
                     /locus_tag="VGE92_14860"
     CDS             513..>2330
                     /locus_tag="VGE92_14860"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1315159.1"
                     /translation="MNLSAPFIKRPIGTTLLTAAVALVGMASFKLLPVSPLPQVDFPT
                     VSVSASLPGASPEIMASSVATPLEREFGRIAAVTEMTAQSSLGQTSVTLQFDLSRNID
                     AAARDVESAINAARSYLPANLPNNPTYRKVNPADAPIMIIALTSDKIAPAGLYDAAST
                     VLQQRLSQIGGVGQVLVGGSSAPAVRINVNPTQLNAYGLQLEDVRTAVAAQTVNEAKG
                     GFADETGNWQIAANDQLLKAKDYSPLIVSFRSGAPVKLSDIAAVSDSVQTVNSMGLSN
                     GKRAALLIIFRQPGANIISTVDAIRATLPLLHASIDPSIELRVALDQTATIRASVSDI
                     ERTLMISIVLVVLVVFVFLREWRSTLIPLVAVPVSIIGSLGAMYLLNYSIDNLSLMAM
                     TISTGFVVDDAIVVLENITRYQEQGMSAAEAASKGAAEIGSTVVSMSLSLVAVFIPIL
                     LMGGIIGRLFREFAVVLSVTILVSLAVSLTTTPMMCSLLLKPTANHGKVYLWSERVFE
                     RILKGYEVSLRWVLERSIVVLLVALATIGLSVYLYVIIPKGFFPQQDTGRLNGNVIAD
                     QSISFQAMQVKIQRLAAIVKSDPAVDTVIAFTGGGGGTTT"
BASE COUNT          425 a          724 c          698 g          483 t
ORIGIN      
        1 gtgctgggcg tgctctatga aagctacatc cacccgatca cgatattatc gactctgccc
       61 tccgcgggcg tgggagcgct gctcgcgttg ctgctgtttc acaacgagct caccgtggtc
      121 gccatcattg gcatcgtgtt gttgatcggc attgtgcaga agaacgccat catgatgatc
      181 gatttcgcgc tcgacgcgca gcgcaaggag cacaagagcg cgttcgatgc cattcacgag
      241 gcctgcctgc tgcgcttcag gccgatcttg atgaccacca tggccgccat gctcggcgcc
      301 atgccgttgg cttttgggca cggcgtcggc tcggagctgc gcagaccctt gggtatcacg
      361 atcatcggag gcttgatatt cagccaaatg ctgaccctgt atacgacgcc ggtcatttat
      421 ctattcttcg acgggctggc tgaacgattc gcccgctggc gtgctgcgca aagcggccga
      481 tcaacagaca ccgcggtaag accgagcgcg acatgaacct gtcggccccc ttcataaagc
      541 gaccgatcgg caccacgctg ttgaccgcgg ccgtcgcgtt ggtcgggatg gcgtcgttca
      601 aattgctgcc ggtatcgccg ctgccgcagg tcgactttcc gacagtctcc gtgtcggcat
      661 ccttacccgg tgcgagcccc gagatcatgg cctcctcggt cgcgaccccg ctcgaacgcg
      721 aattcggccg catcgccgcg gttaccgaga tgaccgcaca aagttcgctg ggccaaacga
      781 gcgtcacgct gcaattcgat ttgagccgca atatcgacgc cgccgcgcgc gacgtggaat
      841 cggcgatcaa cgcggcgcgc agctacttgc cggccaattt gccgaataat cccacgtacc
      901 gaaaggtcaa ccccgccgat gcgcccatta tgatcatcgc gctcacctcg gacaaaatcg
      961 cgcccgccgg tttgtacgat gcagcatcga cggtgttgca gcagcgccta tcgcaaatcg
     1021 gtggcgtcgg ccaggtgctc gtcggcggca gctccgcgcc ggcggtgcgc atcaacgtca
     1081 atcccacgca gctgaacgcc tatggattgc agctcgaaga tgtccgcacc gcggtggccg
     1141 cgcagacggt caacgaagcc aagggcggct tcgccgacga gaccggcaac tggcaaatcg
     1201 cggcgaatga tcagctgttg aaggccaagg actacagtcc gctgatcgtc agcttccgca
     1261 gtggcgcgcc ggtcaagctg agcgacattg ccgcggtctc cgattcggtt cagaccgtga
     1321 actcgatggg cctttccaac ggcaagcgcg cggcgttgct gatcattttt cgtcagccgg
     1381 gcgccaacat catcagcacc gtggacgcca tccgcgccac cttaccgctg ctgcacgcga
     1441 gcatcgaccc gtcgatcgag ctccgggtgg cgctcgatca aaccgccacg atccgcgcct
     1501 cggtgtccga catcgaacgc acgttgatga tctcgatcgt gctggtggtt ctcgtggtgt
     1561 tcgtgttctt gcgggaatgg cgctccacct tgatcccgct ggtggcagtc ccggtgtcca
     1621 tcatcgggtc actgggcgcg atgtacctgc tcaattacag catcgataac ttgtcgctga
     1681 tggcaatgac aatctcaacc gggttcgtgg tggacgatgc catcgtggtg ctggagaaca
     1741 tcacccgcta ccaagagcaa ggcatgagcg cggccgaggc ggcgagcaaa ggtgccgccg
     1801 agatcggatc gacggtggtg tcgatgagct tgtcgctggt cgccgttttt atcccgattc
     1861 tgttgatggg cggcatcatc ggtcgactgt tccgcgagtt tgcggtggtg ttatcggtca
     1921 cgattctcgt gtccttggcc gtctcgctca ccacgacacc gatgatgtgc tcgctgctgc
     1981 tgaagccgac cgcgaaccac ggcaaggtgt atctatggag cgaacgcgtg ttcgagcgga
     2041 ttttgaaggg ttatgaggtc agtctgcgtt gggtcctcga acgctccatt gtcgtgctgc
     2101 tggttgcctt ggccaccatc ggcttgagcg tctacctgta tgtgatcatt ccaaagggat
     2161 ttttcccgca gcaagatacc gggcggctga acggcaatgt catcgccgat cagtcgatct
     2221 ccttccaagc gatgcaggtc aagatccaga gacttgccgc catcgtcaag tccgaccccg
     2281 cggtcgacac ggtgattgcc ttcaccggcg gcggcggcgg gaccacgacc
//
LOCUS       DASWZT010000520         7144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_91213, whole genome shotgun sequence.
ACCESSION   DASWZT010000520 DASWZT010000000
VERSION     DASWZT010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7144
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91213"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(312..833)
                     /locus_tag="VGE92_14865"
     CDS             complement(312..833)
                     /locus_tag="VGE92_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315160.1"
                     /translation="MIDTEVVRLRRLRNTALWARAIAAALDSRQKRRNSLFFRTQLSC
                     WRVARTVTGKLRAHPYPRYQREPGGLHAACTRSGARFLGTVAQRRGRSLQILALELQR
                     VARELDDARALTRSTDLSDDFGRSQVQIRSLIKELDCGVRNEARHEAQSHHEIVPQAA
                     APVDNESWPYLAL"
     gene            complement(1048..2211)
                     /locus_tag="VGE92_14870"
     CDS             complement(1048..2211)
                     /locus_tag="VGE92_14870"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY1315161.1"
                     /translation="MDFSLNDEQQMIVKSTREFVINELYPHEKEVEESGVLREDLHRE
                     LKAKAIEAGLYAANMPVEVGGAGLDTVTWVLYEKELGRTNYALHYSCVGRPSNILLAC
                     AGEQRERYLLPTVRGDRVECLAMTEPGAGSDLRGMKTTAVAVGEDFVINGTKHFISHA
                     DHSDYVILFAATGEESSAHGTRKKMTAFLVDMGHPGFAVRPGYKNVSHRGYTNSILEF
                     NDCRVPKSAILGELHRGFDVANTWLGATRLQVAATCLGRAERALELSTQWAVDRVQFG
                     QQIGKFQGVSFKLADMAVELRAAELLVLEAAWKRDRKIDTDMDMAIAKVKATEMLAMV
                     ADEALQIHGGMGLMSDLPLERIWRDARIERIWDGTSEVQRHIISRGLLRPLGG"
     gene            complement(2222..2845)
                     /locus_tag="VGE92_14875"
     CDS             complement(2222..2845)
                     /locus_tag="VGE92_14875"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY1315162.1"
                     /translation="MPKIVDHARRRDEIAHVACEVVAGHGFEQATVARIARAAGYTTG
                     MVAHYYESKQDIILAALRLILQRIEARLTREREAGEKNLLDVLSESLAIDAQRFTECA
                     FWMAFWGQVLADKKLKRLNIWVHREYMRLFARCFAEHWQEWADWPQAARDQVLRSFVI
                     FINGLTASAVTSPGDWPAAMQVEQLRLQLELLRQWGKRDARSGARVK"
     gene            complement(2854..4338)
                     /locus_tag="VGE92_14880"
     CDS             complement(2854..4338)
                     /locus_tag="VGE92_14880"
                     /EC_number="1.1.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF005716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine 3-dehydrogenase"
                     /protein_id="HEY1315163.1"
                     /translation="MRNVKTLGLLGTGVIGGGWAARALHFGINVIAADVKPEMEEWIR
                     GAVANAEPALARLTAAPLPPKGTLSFTTDLRDMARQADFVQENIPEQLALKQRMLAEV
                     SRHAAADVIIASSTSGLTPTDLQRDMVSPERFCVAHPFNPVYLLPLVELVGGAKTVQT
                     TIDAAAKFFTYIGMHPLHVRREVPGHLTDRLQEALWREILHLVNDGVATTGELDDSIV
                     YGPGLRWAAMGTNLIYHLAGGESGMRHMLAQFGPCLKWPWTKLEAPELTDTLIDRMVE
                     GTQAQAAGRSIRELERLRDDYLVAIQQVLRQYDIGAGSTLRALEARLHHDAARATAAT
                     LKDSAKPLRLMDATVRAEWVDYNRHMSDFRYVQLFGDAIDVLFRQVGVDEAYRATGRM
                     YYTVENHIRHFGEAKAGEGLYVTAQLLGVDAKRLHVLQRVYRSRDDREIASAEQIYLH
                     VDTAAAKASPADAAIRAKLEALATAHAQLPVPSESGKPISLKRS"
     gene            4455..6056
                     /locus_tag="VGE92_14885"
     CDS             4455..6056
                     /locus_tag="VGE92_14885"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY1315164.1"
                     /translation="MGNKIAVPSPSRRELLRMIGITAGNAAMYHAMSSLGFAAESPYE
                     GPADLHGAPRGASVLILGAGLAGMSAAYELQRAGYRVQVLEYNGRAGGRNWTLRGGDA
                     YTELGGFKQECQFDKDLYINPGPWRIPYHHRGMLDYCKRLRVPLESFVQVNYNAYLHS
                     SRSFGGKPQRFREIKADYQGHVAELLAKATQQNALDAAVSKEDQEKLLESLRGWGALD
                     KSNVYSGSGERRGYQKNPGGGASARPIASTPIGLGDLLNSGLWSGIAAGDVFDMQTAL
                     FQPVGGMGRIGEAFGRELGPLIRYDAKVVDIHQDERGVTVAYEDARSLGQVQQARADW
                     CVCTIPLPILAQIPMNVGTALASAVSAVPYISAVKVGLQFKRRFWEEDEQIYGGITYT
                     DLPIGNIGYPNSGFHASGKGVLLGAYMFGANAAEFTSMTPPERVQKAVDYGKQIHRQY
                     REEFDNGVAVAWHRVPSAMGCAGMWTSDSRAKHYDDLCAFDGRIVLAGEHASFIGGWQ
                     EGAVTSALDAIGRLHQRAVAQGEKA"
     gene            6059..6496
                     /locus_tag="VGE92_14890"
     CDS             6059..6496
                     /locus_tag="VGE92_14890"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY1315165.1"
                     /translation="MHLRRVGAGLGALACACLAAAVALSDSPSVTSSAISSVGGMQVY
                     QHLCQGCHMADAQGAVGAGHYPKLAGNPALRSWQYVALTVLNGKNGMPAFGVRLSKAA
                     DFGSIALDDARVAEVVNYVRSHFGNRWQDKATATEVAALPHPP"
     gene            6614..7138
                     /locus_tag="VGE92_14895"
     CDS             6614..7138
                     /locus_tag="VGE92_14895"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY1315166.1"
                     /translation="MHKDETRRRGMMPALGLLAGALSGGAVSAQDVVRTPLPDGNPFP
                     ISAAVTVRAGLDTVYVSGALPTATNKDAPKGTPPVYGDMQAQTVSVLTSIKGTLAKLG
                     LGMGDVVKMTVFLVADPAYDNKLNFPGLMAGYSQFFGTKDQPNKPARSAVQVAALVAP
                     GALLEVEVIAAKPR"
BASE COUNT         1308 a         2278 c         2220 g         1338 t
ORIGIN      
        1 atgccgatgg caatctgctg cttagtgccc acggcgatgc tcccatttga tttggcgacg
       61 tccgggaggc acgcatccga tcaccttgag cgcctgatgc cacgccgccg cttgcggcgc
      121 cggcgccgga ggatcatacg gggcaatggg aggtcgctcg gggcttggag gactgggaac
      181 gttctcgctg cgcatctcga actccttagc ttggatctca gggtcgcagt atcagggcgc
      241 cggagcgcgg cagcgatcag ctaaatacgc caactacatg taggacaaat catacgccgc
      301 tgcctgcaga gctaaagagc caggtagggc caactctcgt tatcgaccgg cgccgcggct
      361 tgcgggacga tttcatggtg cgactgcgcc tcatgcctcg cctcgttgcg aactccgcag
      421 tctaattcct tgatcaggct acggatctgt acttgcgagc ggccgaagtc atcgctcaaa
      481 tccgtcgacc gggtgagggc acgcgcatca tccaattcac gcgccacgcg ctgcagctcc
      541 agcgcgagaa tctgcaggct gcggccgcga cgctgcgcga ccgtcccgag gaaacgcgcg
      601 ccgctgcggg tacaggccgc atgcaagccg ccgggttcgc gctgataacg cggataagga
      661 tgtgcgcgca gcttgccggt cacggtgcgg gcgacccgcc agcaactcag ctgggtgcgg
      721 aaaaatagcg aattgcgccg cttttgccgc gaatcgagcg ccgcggcaat tgcgcgcgcc
      781 cacagcgccg tgttgcgcaa ccgcctgagg cgtaccactt ccgtatcgat catgtgccat
      841 ccatccgagt gttgatccct tacctcccta acgcaccaag ctgcacccgg tgcacatggg
      901 aggattggac gtattttcag gcacctgggg catcgcgtct gtaggacaac accccatgaa
      961 tattggcata tgacgggtcc ggatcgcata tgacgcatcg gcgcaggtat atgtcgcgag
     1021 cgaacgcctg atgttaaata aggcgcctca gccgcccaag ggtcgcagta gaccgcggga
     1081 aatgatatgg cgctgcactt cgctggtccc gtcccaaata cgttcgatgc gcgcgtcgcg
     1141 ccaaatgcgc tccagcggca agtcgctcat cagccccatg ccgccgtgta tctgcagcgc
     1201 ctcatccgcc accatggcca gcatctcggt ggctttgacc ttggcgatgg ccatgtccat
     1261 gtcggtgtcg attttgcgat cccgtttcca tgctgcctcc aacaccaaca gctccgcagc
     1321 ccgaagctcc accgccatgt cggccagctt gaaactcacg ccttgaaatt tgccgatttg
     1381 ctgaccgaat tgcacacggt cgaccgccca ctgggtcgac agttccaacg cgcgttcggc
     1441 gcgtccgagg caggtcgccg cgacctgtag ccgagtcgct cccagccagg tgttcgcgac
     1501 atcgaagccg cggtgtaatt cgcccaaaat ggcgctcttg ggcactcggc aatcattgaa
     1561 ctccagaatg ctgttggtat agccgcggtg cgaaacattc ttgtaccccg gtcgcacggc
     1621 aaaacccgga tgccccatat ccaccaagaa tgcggtcatt ttcttgcgcg taccgtgcgc
     1681 gctcgactcc tcgcccgtgg ctgcgaacag aatgacgtaa tcggagtggt cggcgtggct
     1741 gatgaaatgc ttggtgccgt tgatgacgaa gtcctcgcct accgcgacgg cggtggtctt
     1801 catgccgcgc agatccgacc ccgcgcccgg ctcggtcatg gccaggcact cgacccgatc
     1861 gccgcgcacc gtcggcagca ggtagcgctc gcgttgttcg ccggcgcagg ccagcaggat
     1921 attcgacggg cggccgacgc aggagtaatg cagagcgtaa ttggtgcgtc ccaattcctt
     1981 ctcataaaga acccaggtca ccgtatccaa gcctgcaccg ccgacctcga ccggcatgtt
     2041 ggccgcatac aagccggcct cgatggcctt cgccttgagc tcgcggtgca agtcctcgcg
     2101 caacacaccg ctctcttcga cttctttctc atgcgggtag agctcgttga tgacgaactc
     2161 ccgggtcgac ttgacgatca tctgttgttc atcgttgagc gaaaaatcca tggctaattc
     2221 ctcatttgac acgtgcgccc gagcgcgcat cgcgttttcc ccattgacgc agcagctcca
     2281 gttgcaggcg cagctgttcg acctgcatcg cggcgggcca atcgcccgga ctcgtgaccg
     2341 cgctcgccgt gagcccgttg atgaatatca cgaaggagcg cagtacttga tcgcgtgccg
     2401 cctgcggcca atcggcccat tcttgccaat gctcggcaaa gcaacgcgcg aacaagcgca
     2461 tgtactcgcg atgcacccag atgttcagcc gcttgagttt cttgtcggcc aacacctggc
     2521 cccaaaacgc catccagaac gcacactcgg tgaagcgttg cgcatcgatg gcgagcgatt
     2581 ccgacaggac atccagcagg ttcttctcgc cggcctcgcg ctcgcgggtc agccttgcct
     2641 cgattcgctg cagtatgagc cgcagcgccg cgagaatgat gtcctgcttg gactcataat
     2701 aatgagccac catgccggtg gtgtagcccg ccgcgcgcgc gatgcgcgcg accgtagcct
     2761 gttcgaaccc gtggcccgcg actacctcgc aggcgacgtg ggcgatttca tcgcgccgcc
     2821 gtgcatgatc gacgatcttc ggcatggcgc ggcttagctt cgcttgagtg aaatcggttt
     2881 gcccgattcg gacggtaccg gcaattgcgc atgcgccgtg gcgagtgcct cgagcttggc
     2941 gcgaatcgca gcatccgcgg gcgacgcctt ggcggcagcc gtgtcaacgt gcaggtatat
     3001 ctgttcggcg cttgcgattt ctcgatcgtc gcggctgcgg tatacgcgtt gcaatacgtg
     3061 caggcgcttg gcgtcgacgc cgagcagttg cgcggtgaca tacagacctt cgcccgcctt
     3121 cgcctcgccg aaatgccgga tgtgattttc gaccgtgtag tacatgcgtc cggtggcacg
     3181 atacgcctcg tcgaccccca cttgccgaaa caacacgtca atggcatcgc cgaaaagctg
     3241 aacgtagcgg aagtccgaca tgtgccggtt gtagtccacc cattcggcac gtaccgttgc
     3301 atccatgagc cgcagcggct tggccgaatc cttcagcgtc gcggccgtgg cacgcgccgc
     3361 gtcatggtgc agccgcgcct cgagcgcccg cagggtggaa cccgctccga tgtcgtactg
     3421 cctgagcacc tgctgaatcg caaccagata gtcgtcgcgc aggcgctcga gttctcggat
     3481 cgagcgtccg gcggcctgcg cctgcgtgcc ctcgaccatc cgatcgatca acgtatcggt
     3541 caattccggt gcttcgagtt tagtccaagg ccacttgagg cacggtccga actgcgccag
     3601 catgtgccgc atgccgcttt cgccgcccgc caggtgatag atcagattgg tccccatcgc
     3661 agcccagcgc agccccggcc catacacaat ggagtcgtcg agttcgccgg tggtggccac
     3721 cccgtcgttc acgaggtgca aaatctcgcg ccatagggcc tcctgcaggc ggtccgtcag
     3781 atggccggga acttcgcgcc ggacatgcag cgggtgcatc ccgatgtagg taaagaactt
     3841 ggccgccgcg tcgatggtcg tctgcaccgt cttcgcgccg ccgaccaatt cgaccagcgg
     3901 cagcaagtac acggggttga agggatgcgc cacgcagaag cgctccggcg acaccatgtc
     3961 gcgttgcaaa tcggtggggg tcaagcccga ggtgctcgag gcgatgatga cgtccgccgc
     4021 agcgtgccgg ctcacttccg ccaacatcct ttgcttcaaa gccaattgtt ccgggatgtt
     4081 ctcctgtacg aaatcggctt gccgcgccat gtcgcgtaga tccgtggtaa acgacagggt
     4141 gcccttcggc ggcaatggcg ccgcggtgag ccgcgccaac gcgggctcgg cattggcaac
     4201 cgccccgcgg atccattctt ccatctcggg cttgacgtcc gcggcgatga cgttgattcc
     4261 gaaatgcagg gcgcgcgccg cccagccgcc gccgatcacg ccggtgccga gaagtcccaa
     4321 ggtcttcaca ttgcgcatgt ttgccagcct ctcaacgcga gttttaatgt cacgagtata
     4381 atataaaaaa cccggcttgt atgcggcgcg gcttcgtgtg ataacccgtc cacacccttg
     4441 atgtagaggt agcgatggga aacaagattg ccgtgccctc ccccagccgg cgcgagctgt
     4501 tgcgcatgat cggcatcacc gcgggcaatg ccgccatgta ccacgcgatg agcagcttgg
     4561 gatttgccgc cgagtcgccc tacgaggggc cggcggactt gcacggcgcg ccgcgcggcg
     4621 cttccgtgct gatcttgggc gcgggtctcg ccgggatgag cgcggcctac gagttgcaga
     4681 gggccggcta ccgggtgcaa gtgctcgaat acaacggccg agccggcggc cgcaactgga
     4741 ccttgcgcgg cggcgatgcc tacaccgaac tcggcggatt caaacaggag tgccaattcg
     4801 acaaggattt gtacatcaac ccgggtccgt ggcgaattcc gtaccaccat cgcggcatgc
     4861 tcgactactg caagcgcctg cgggtgccgc tcgagtcctt cgtgcaagtg aattacaacg
     4921 cctatttgca cagcagccgc tcgttcggcg gcaaaccgca gcgctttcgc gaaatcaaag
     4981 ccgactacca gggtcatgtc gcagagttgt tagcgaaggc cacgcaacaa aatgccctcg
     5041 acgcagcggt gagcaaggaa gaccaagaga agttgctcga atcgctgcgc ggttggggcg
     5101 ccctcgacaa gagcaacgtt tattcgggca gcggcgagcg ccgcggctac cagaagaatc
     5161 ccggcggtgg tgcgagcgcc aggccgatcg catcgacgcc gatcggcctc ggcgatctat
     5221 tgaactcggg gctttggtcc ggcatcgccg cgggcgatgt attcgacatg cagacggccc
     5281 tgttccagcc ggtgggcggc atgggccgaa tcggcgaggc cttcggccgc gaattgggac
     5341 ccctcatccg ctacgacgcc aaggttgtgg atattcacca ggacgagcgc ggcgtcacgg
     5401 tggcctacga ggatgcacgc agcttaggcc aggtgcagca ggcgcgggcg gattggtgcg
     5461 tatgcaccat tcccttgccc attttggcgc agattcccat gaatgtcggc acggccctgg
     5521 catcggcggt gtcagcggtc ccgtatatct ccgccgtcaa ggtgggattg cagttcaagc
     5581 ggcgcttctg ggaggaggat gagcagatct acggcggaat cacctacacc gacttaccga
     5641 tcggcaacat cggctatccg aacagcgggt ttcacgcgag cggcaagggc gtgctgctgg
     5701 gcgcctacat gttcggcgcg aacgcggcgg agttcacgtc gatgacgccg ccggaacgcg
     5761 tgcagaaggc ggtggattac ggcaagcaga ttcaccgcca atatcgcgag gagttcgaca
     5821 atggagtcgc cgtggcctgg caccgcgttc cctccgcgat gggatgcgcc ggcatgtgga
     5881 ccagcgactc gcgcgccaag cactacgatg atttgtgcgc cttcgacgga cggatcgtct
     5941 tggccggcga gcacgcctcg ttcatcggtg ggtggcagga aggcgcggtg acgtcggcct
     6001 tggatgccat cggccggctg catcagcgtg cagtggcgca gggagaaaaa gcttgaacgt
     6061 gcatttgcgg cgtgttggtg ccgggctcgg tgcgctggcg tgcgcttgcc tcgccgccgc
     6121 cgtggccttg tccgacagcc cctcggtcac gtcgtccgcg atcagcagcg tcggcggcat
     6181 gcaggtttat cagcatcttt gccagggctg ccacatggcg gatgcgcaag gcgccgttgg
     6241 cgctggtcac tatccgaagc tcgccggaaa tcctgcgttg cgctcctggc agtacgtggc
     6301 gctcacggta ctcaacggca agaacgggat gccggccttc ggcgtccggc tctccaaggc
     6361 ggcggatttc ggttcgatcg cgctggacga cgcccgcgtc gccgaggtgg tcaattacgt
     6421 gcgcagccac ttcggcaacc gctggcagga caaggcaacc gcgaccgagg tcgctgcatt
     6481 gcctcatcct ccctgatcta gacttagaac cggtattgcg ccttcaggca gtcgtcgcag
     6541 aaacagctgg acagcaaggc aagcgagttg cgatcttagg cgcccgatca gtcacgcagc
     6601 acaggagttg cacatgcaca aagacgaaac tcgacgccga gggatgatgc cggccttggg
     6661 gctgttggcg ggcgcactga gtggcggcgc tgtcagcgcg caagatgtcg tgcgcacgcc
     6721 gcttccggac ggcaacccgt ttccgatctc cgcagccgtc acggtgcgcg ccgggctcga
     6781 tacggtttac gtgagcggcg cgttgccgac cgccaccaac aaggatgcgc ccaagggcac
     6841 gccgccggtc tacggcgaca tgcaggcgca aaccgtcagc gtcctcacct cgatcaaggg
     6901 cacgctggcg aagctcggtc ttggcatggg cgacgtcgtg aaaatgacgg tgttcctggt
     6961 ggccgacccg gcctatgaca acaagctcaa ttttccggga ttgatggcgg gctacagcca
     7021 gttcttcggc accaaggatc agccgaataa gcccgctcgc agtgccgttc aagtggcggc
     7081 tttggtcgcg cccggcgcgc tgctcgaggt cgaagtcatc gccgccaagc cgcgctgagc
     7141 gccc
//
LOCUS       DASWZT010000521         9838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_71945, whole genome shotgun sequence.
ACCESSION   DASWZT010000521 DASWZT010000000
VERSION     DASWZT010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9838
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71945"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..1167
                     /locus_tag="VGE92_14900"
     CDS             274..1167
                     /locus_tag="VGE92_14900"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY1315167.1"
                     /translation="MSWRGWSAFAALGIIWGLPYFFIKLSVQELSPVVVAFGRVALGT
                     MILLPIAWRRGALRSVGKHKAAIGAFAMAEFAIPFLVISFGERWISSSVTGILIAMVP
                     LSIALIQRFFGVHERLGTWRILGLCLGFVGVAALLGFGTISGPLGWTGVACMLIATLG
                     YAIGPLIIQRHLRALDPFGPLAASLGVASAVLFVPAMLALPSQLPSPLALISMAILGV
                     VCTAVAMLLMFYLVNHAGASRAAVITFINPAVATLLGVWLLHERLGLGGILAFALILL
                     GSWLATRGSVAQPGRSGEALA"
     gene            1180..2469
                     /locus_tag="VGE92_14905"
     CDS             1180..2469
                     /locus_tag="VGE92_14905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY1315168.1"
                     /translation="MAPSSESEAISVAPLWMRSAAPPRPATTLPSRVDVLVIGAGYTG
                     LAAARETAAAGRSTWVLEAGELGTGCSGRNGGQVAYSIKPSFGALTTKYGADLAFRIC
                     REGHDAVAHLKALAREADFGWREDGCFFGAHTRRHFAAMVRDAASQPRGLEQRINVVP
                     RAAQHSEIASDFYHGGCVYPDDASVDPMRLLRVLLERAQRSGASLLERCPASGVNPVR
                     DGFEVLTPRGTVHARQVLLATNGYSGPLSPWHRRRVIPIGSYQIATEALGTERVSALI
                     PRRRNIVDSRRVVVYFRPSADGERIVFGGRAALAEKDPLACVSRLKQMLQRIFPQLAA
                     VRISNAWLGWVAYTFDTLPHLGQHNGIFYCMGYCGQGVPLAPYFGMRIGQQMLGAAQG
                     RTALDGLPFPTRPYYFGKPWFLAPSVFVYRSIDALGL"
     gene            2483..3946
                     /locus_tag="VGE92_14910"
     CDS             2483..3946
                     /locus_tag="VGE92_14910"
                     /inference="COORDINATES: protein motif:HMM:NF000658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEY1315169.1"
                     /translation="MQRASYANLPDRFFAHIEPVPVAKPRLLKFNHALCAELGLGICE
                     LDAEALAGIFSGNVVPAAVVPIATAYAGHQFGHFVPQLGDGRAILLGQAQDRAGVLRD
                     IQLKGSGRTVYSRGGDGRAALGPVLREYLVSEAMHALGIPATRALAAVATGESVLRED
                     VLPGAILTRVAASFVRVGTFQYFAARDDVEAVRLLADYVIARHFAHIKTHEVPYLALL
                     RAVSDAQASLIASWMHVGFIHGVMNTDNMAVSGETIDFGPCAFMDGYDKAAVFSSIDR
                     QGRYAFGNQPHAALWNLARFAETLLPLLDPVDERAVELASAELATFSPRFAEHWLHGM
                     RRKLGLAAGEDGDAALAEDLLDTMQRNQADFTLTFRALADAAEGAAGLAAARSLFANP
                     HDFDEWAHRWQLRVGREPGDPTSRAASMRQVNPLFIPRNHRIEQVIRAAVERDDFGPF
                     AQLSRVLAQPYHGQPGFETYADPPQPEERVLQTFCGT"
     gene            complement(3925..4821)
                     /locus_tag="VGE92_14915"
     CDS             complement(3925..4821)
                     /locus_tag="VGE92_14915"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEY1315170.1"
                     /translation="MLAPEAFITCALTGSADSVGKNPHVPVTPIEIADAALDAHAAGA
                     AIVHIHVRDPRSGKSSRDPQLFNEVVARIRSRNSAVILNLTGGMGGDLALGPPDSPLE
                     FAAGTDFVGPAPRMAHILQLRPEIGSLDSGSLNFDELLYGTTPAYLRTMARAYREHNV
                     RPELEVFELGHIELAKQLMAEGLIERPALFQLCLGIKYGAPATAEAMLAMRDALPSGS
                     TWSAFGLGRMQMPMVAQAVLLGGNVRVGLEDNLYLDKGVVATNAQLVTRARAIIELLG
                     VRVLSSDETRERLRLAQVPQKV"
     gene            4929..6701
                     /locus_tag="VGE92_14920"
     CDS             4929..6701
                     /locus_tag="VGE92_14920"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein DipZ"
                     /protein_id="HEY1315171.1"
                     /translation="MTVVFLVFLGGVLTILSPCILPVLPFVFARAEQRFLTNGLPMLG
                     GMAVTFAAIATLAAVGGAWAVRLNQYGRVLALLLLTAFAATLLSRRLADWLARPFVAL
                     GNRLLQSAPQGAASLGVLQAVLLGVATGLLWAPCAGPILGLVLTGAAISGPNPRTTLL
                     LFAYAAGAATSLGVAILAGGRVFAVLKKSLGAGEWVRRGLGVAVLLAVVAIASGWDSS
                     VLTRWSLGGTNRLESSLIDRIRGGAPPGASPRNANGTMAVHGPAAPDAALPIEGDIPS
                     LSGATAWLNSGPLTPADLRGKVVLVDFWTYSCINCLRALPYVSGWYEKYQAHGLIVIG
                     VHAPEFAFEKNVDNVRRAVADLHITYPVALDNDYAIWQGFNNQYWPAHYFIDSQGHIR
                     AHHFGEGSYDESEATLRQLLSEAGYTDLPPAGMQSAMAVGAQAAADPAHMQSPETYIG
                     YRRAENFASPGGFAQDRPKNYAEPAELRLNQWALAGPWLVDAEKAQLRGVSGKIEFRF
                     FARDLHLVAGPGGDGKRVRFRVLLDGAAPLANHGADTDENGEGVIDQQRLYQLIRQSG
                     AVGEHVFTIEFLDDGVQAYSFTFG"
     gene            6710..7300
                     /gene="msrB"
                     /locus_tag="VGE92_14925"
     CDS             6710..7300
                     /gene="msrB"
                     /locus_tag="VGE92_14925"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY1315172.1"
                     /translation="MSFKHRPNRSAAALSRRTFIAGLGAAALATYGCKPSAATLSAPE
                     QSADVTIEEFSAAGKSLGHVRVAKVVKSAAQWREQLSPLAFSVTREQGTEIAFTGEYD
                     GNHADGLYRCVCCDTALYDSKTKFDSGTGWPSFWQPISALNVVKNADNSLGMQRDEIS
                     CRRCAAHLGHVFDDGPRPTGLRYCMNSVALHFVARA"
     gene            7314..8024
                     /gene="msrA"
                     /locus_tag="VGE92_14930"
     CDS             7314..8024
                     /gene="msrA"
                     /locus_tag="VGE92_14930"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY1315173.1"
                     /translation="MMSFWKYRTSLGLGAMLLLAALWGSWRSPLFGAEAAVAIAPPAV
                     DNPRTAGPAQTAVLAGGCFWGVQGVFEHVRGVQKVVAGYAGGERSTAQYETVSSGTTG
                     HAESVKITFDPAQISYGQILQIAFSVVHDPTQLNRQGPDVGTQYRSAVFYVDDAQKHI
                     AQAYIGQLDKSHAFPRPIVTRVDPLQGFYAAEDYHQDYLIHNPDAPYIAYYDIPKIEN
                     FKQAFPQLYSGRPVLAHN"
     gene            8034..8807
                     /gene="modA"
                     /locus_tag="VGE92_14935"
     CDS             8034..8807
                     /gene="modA"
                     /locus_tag="VGE92_14935"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF007958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY1315174.1"
                     /translation="MKRFAGVWLTCCVSLWAQAAFAAAADKPPLTVFAAASLTNVLQE
                     LGDGFTKETQIPIRFSFAASSALARQIENGSPADIFFSADLEWMDYLQTRGLIQRDSR
                     HNLLGNRLVLIAPTESAIKLKIEPHFALAAALGSGHLATGDPDSVPVGRYAREALTSL
                     GVWNDVADRVVRADSVRSALAFVDRSEAPLGIVYATDALIDKRVRVVDVFPDNSHLPI
                     LYPIALTRAAKPDAAKFVSYLSGPAGGVAFKAYGFTPPR"
     gene            8838..9236
                     /locus_tag="VGE92_14940"
     CDS             8838..9236
                     /locus_tag="VGE92_14940"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEY1315175.1"
                     /translation="MSETAAVDGLKILPTSHSVAEVLQRVQASARERGLTIFAQIDFS
                     GDAQRSGLTLPPAGMIILGNPRAGTPLIAATPTVAIDLPLKILAWQDANGKTWVGYNA
                     AEYLQARHGFPSALLKNIAALDALAAAAAA"
     gene            complement(9255..9722)
                     /locus_tag="VGE92_14945"
     CDS             complement(9255..9722)
                     /locus_tag="VGE92_14945"
                     /inference="COORDINATES: protein motif:HMM:NF024779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HEY1315176.1"
                     /translation="MQGLVEIGGFFKEFELSIIAADERDVVRLRTNVRGEDDYLYRLH
                     LSPAAMRSLFLGYIDQANSLLHTPRFYNTITINCTTLVYHMMKRIVGYLPLSYRLLFS
                     GYLPGYVYEVGGLDSRYSLEQLRALGRITDRAKQADRSATFSADIRRGVPEIP"
BASE COUNT         1680 a         3066 c         3191 g         1901 t
ORIGIN      
        1 cgccctgtcc gcgtaccaag tagtgcaaag aatgttcttg gtttgaaaag aaggccatcg
       61 cccgatctcc tggctcgcgt ccgtctcgaa tcaccaccca tggtgaatca caacgtaatc
      121 ttaaagccac ggttgacaga cgccgaccgc cgccggcccg tcgcggcggc ggtgtttcgc
      181 cgtacgtggt gtaggcaagc gctgaccggc ggcgaatccc tgttaccata actgcggcga
      241 tcaacggcaa ttcggcattc aagggcttaa cgagtgagtt ggcgaggttg gagtgcgttc
      301 gcggcgctgg gcataatctg gggcctgccg tactttttca tcaaactgtc ggttcaggaa
      361 ctgtcaccgg tggtggtcgc gttcggccgc gtggcattgg gcacgatgat cctgttgccg
      421 atcgcctgga ggcgcggcgc attgcggtcg gtggggaagc acaaagcggc gatcggcgca
      481 ttcgccatgg cggaatttgc aattcccttt ttggtgattt ccttcggcga gcgctggatc
      541 agttcgtcgg tgaccggaat cctcatcgcc atggtgccgc tgtcgatcgc gctcatccaa
      601 cgcttcttcg gcgtgcacga gcgcctgggt acgtggcgaa ttctcggact gtgcctggga
      661 ttcgtcggcg tcgcggcgct gctcggattc ggcaccattt ccgggccgct cggctggacc
      721 ggggtcgcct gcatgctgat cgcgacgctc ggctatgcga tcggcccgct catcattcaa
      781 cgccatctgc gcgcgctcga tccgttcggt ccgctcgccg cgagcttagg cgtagcgagc
      841 gcagtgctgt tcgtacccgc catgctggcc ttgccgtcgc agttgccatc gccgctggcg
      901 ctgatatcga tggccatact cggcgtcgta tgcaccgccg tggccatgtt gttgatgttc
      961 tacctggtga atcacgccgg cgcatcgcgc gcggccgtga tcacgtttat caatccggcg
     1021 gtggcgacct tgctcggcgt gtggttgctg catgagcgcc tggggctcgg cggaatcctg
     1081 gctttcgcgc tgatcttgtt gggatcgtgg ctcgcgaccc gcggcagtgt cgcgcaaccc
     1141 ggtcgcagcg gcgaagcgtt ggcttgaagc gcaaacccta tggcgccgtc atcggaaagc
     1201 gaagccatat cggtggcacc cttgtggatg cgcagcgccg cgccgccgcg gcccgcgaca
     1261 accctgccga gccgggtcga tgtgctggtg atcggcgctg gttacacggg tcttgcggcg
     1321 gcccgcgaga ccgcagccgc gggccgctcg acgtgggtgc tcgaggccgg cgagctcggc
     1381 acgggctgct ccggccgtaa cggcggccaa gtcgcctata gcatcaagcc gtcattcggc
     1441 gccctgacga caaagtacgg cgccgatcta gcgttcagaa tttgccgaga gggccatgat
     1501 gccgtggcgc atctcaaggc gttggcgcgc gaggcggatt tcggttggcg cgaggacgga
     1561 tgttttttcg gcgcgcacac ccgccggcat ttcgcagcca tggtgcgcga cgccgccagc
     1621 cagccgcgcg gactcgaaca acgaatcaat gtcgtgccgc gcgccgcaca gcacagcgag
     1681 atcgccagcg acttctatca cggcggctgc gtgtaccccg acgatgcatc cgtcgatccc
     1741 atgcgcctgc tgcgggtgct gctcgagcgc gcgcagcgca gcggtgcttc gttgctggag
     1801 cgctgcccag cctcgggcgt caatccggtg cgggacggat tcgaggtgct cacgccgcga
     1861 ggaacggtgc atgcccggca agtgctgctc gccaccaacg gatactcggg cccgctgtcg
     1921 ccatggcacc ggcgccgggt gattcccatc ggcagctatc aaatagccac cgaggccttg
     1981 ggcacggagc gggtgagcgc gctgattcct cgcaggcgca acatcgtcga ttcgcgccgc
     2041 gtggtcgtgt acttcaggcc gtcggccgac ggcgagcgca tcgttttcgg cggtcgagcc
     2101 gccttggcgg agaaagatcc gctcgcgtgt gtgtcacgcc taaagcagat gctgcagaga
     2161 atatttccgc aattggcggc cgtgcgcatc agcaacgcat ggctgggatg ggttgcgtat
     2221 actttcgaca ctttgccgca tctcggccaa cacaacggca tcttctactg catgggctac
     2281 tgcggccagg gcgtaccgct cgccccctat ttcggcatgc gcatcggcca gcaaatgctc
     2341 ggagcggcac agggccgcac ggcgctcgat gggctgccgt tcccgacgcg accgtattat
     2401 ttcggcaaac cctggtttct cgcgccctcg gtattcgtct atcgctcgat cgacgccctc
     2461 gggctttgaa ccgcacaaat ccatgcaacg agcaagctac gccaatttgc ccgatcgctt
     2521 cttcgcgcac atcgagccgg tgccggtggc gaagccgcgc ctgctgaaat tcaatcacgc
     2581 gctgtgcgcc gaactcggcc tcggcatctg cgagctcgat gccgaggcgc tggcgggaat
     2641 cttttccgga aatgtcgtcc cggccgcagt tgtgccgatc gcgacggcct atgcaggcca
     2701 tcagttcggc cacttcgtgc cgcagctcgg cgatgggcgc gcgattcttc tgggtcaggc
     2761 gcaggaccgt gccggcgtgc tcagagacat tcaattgaag ggatccggac gcaccgtgta
     2821 ttcccgcggc ggcgatggcc gcgcggcgct cggacccgtg ctgcgagaat atctcgtcag
     2881 cgaggcgatg catgcgctcg ggatacccgc gacacgcgct ctggcggccg tcgccaccgg
     2941 cgaatcagtg ctgcgcgagg acgttctgcc gggcgccatc ctaacgcgcg tggccgcgag
     3001 cttcgtgcgc gtcggcacct ttcagtattt cgcggcgcgc gacgacgtcg aggcggtgcg
     3061 cctgctcgcc gattacgtca tcgcgcgtca cttcgcgcat atcaagacgc atgaagtgcc
     3121 gtacttggcc ttgttgcgcg ccgtgagcga cgcccaggca tcgctcatcg cgagttggat
     3181 gcacgtcggc ttcattcacg gcgtcatgaa caccgacaac atggccgtgt cgggtgaaac
     3241 catcgatttt ggtccgtgcg ccttcatgga tggctacgac aaggcagcgg ttttcagttc
     3301 gatcgaccgg caaggccgct atgcctttgg caatcaaccg cacgccgcgc tgtggaatct
     3361 cgctcgattc gccgaaacgc tgctgccctt gctcgacccg gtcgacgagc gggcggtcga
     3421 actcgcaagc gcggaactcg cgaccttctc cccgcgcttc gccgagcatt ggctccacgg
     3481 catgcggcgc aagctcggtc ttgcggccgg cgaggacggc gacgcggcac tcgccgagga
     3541 tctgctcgat accatgcagc ggaatcaggc cgatttcacg ctcaccttcc gcgccctggc
     3601 cgacgcggcc gagggggccg caggcttggc ggcggctcgt tcgctgtttg ccaatccgca
     3661 tgacttcgac gagtgggcgc accgctggca gctgcgcgtc gggcgagaac cgggagatcc
     3721 gacgtcgcgt gcggccagca tgcgccaggt caacccgctg ttcattccgc gcaaccatcg
     3781 gatcgaacaa gtcatccgcg cggcggtgga gcgcgatgat ttcggcccgt tcgcacaact
     3841 atcccgcgtg ttggcgcagc cgtaccacgg ccagccggga ttcgaaacct atgcggatcc
     3901 gccgcagccc gaagagcgcg tgcttcagac cttctgcgga acctgagcga gccgcagccg
     3961 ttcacgcgtc tcgtcagagg acaaaacccg cacgcccagg agttcgataa tggcgcgagc
     4021 gcgcgtcacc aattgggcat ttgtcgcaac gacccccttg tcgaggtaga gattgtcctc
     4081 gagcccgacg cgcacattgc cgccgagcaa taccgcctgc gcgaccatgg gcatttgcat
     4141 tcggccgaga ccgaacgcag accaggtgga gccggagggc agcgcatcgc gcatggcgag
     4201 catcgcctcg gcggtcgccg gtgcgccata cttgatgccg aggcacaatt ggaacagagc
     4261 gggccgctcg atcagtcctt ccgccatcag ctgcttggcc agttcaatgt ggccgagctc
     4321 gaatacttcc aactcgggac gcacgttgtg ttctcgataa gcacgcgcca tggtgcgcag
     4381 atacgcaggc gtggtgccgt agaggagttc gtcaaaattg agcgaacccg agtcgaggct
     4441 gccgatctcc ggcctcagct gcagtatgtg cgccatgcgc ggcgccggcc ccacgaagtc
     4501 ggtgccggct gcgaactcga ggggtgaatc cggcggaccc aaggcgaggt cgccgcccat
     4561 tccgccggtc aagttcaaga tcacggcgct gtttcggctt ctgattcgcg ccacgacctc
     4621 gttgaaaagc tgcggatcgc ggctcgactt gccgctacgc gggtcacgga cgtgaatgtg
     4681 gacgatcgcc gcgccggccg catgcgcgtc gagcgccgcg tccgcgatct cgatcggggt
     4741 tacgggcacg tgcggattct tacccacgga atcggccgat ccggtgagtg cacaggtgat
     4801 aaaggcctca ggcgcgagca tggcgcaccg ggttcacacc ggccaaaaaa aaggcacgaa
     4861 cataacctgg ctctgctgca attaatcgac cgtggcaccg gtcttaagga ttgagagcgt
     4921 acaacaaaat gacagtcgtt tttttggtgt ttttgggcgg cgtactcacg attttgagtc
     4981 cgtgcatcct gccggtattg cctttcgtgt tcgcacgtgc cgagcaaagg ttcctgacga
     5041 acgggctgcc gatgctgggt ggcatggccg tcaccttcgc cgcaatcgcg acgctcgccg
     5101 ccgtgggcgg cgcctgggcg gttcggctca accagtacgg ccgcgtgttg gcgctgctat
     5161 tgctcacggc attcgccgcg accctgttgt cccggcgcct ggccgattgg ctggcccgcc
     5221 cgtttgtggc tctcggcaat cgattgctgc aaagtgcccc gcagggggct gcaagtcttg
     5281 gcgtactgca ggcggtgctg ctcggcgtgg cgaccgggct tctgtgggca ccgtgcgcag
     5341 gtccgattct cggcttggtg ctcaccggcg ccgccatttc gggtccgaat ccgcgcacca
     5401 ccttgttgct gtttgcctac gcggcgggcg ccgccacatc gttgggcgtt gccatccttg
     5461 ccggtggacg ggttttcgcg gtgctcaaga aatcgctcgg cgccggcgaa tgggtgaggc
     5521 gcgggttggg cgtcgccgta ttgctcgcgg tcgtcgcaat cgcgtcgggt tgggacagca
     5581 gcgtgctcac gcgctggtcg cttggcggca ccaatcggct ggagtcatcg ctcatcgacc
     5641 ggatcagggg cggagcgccg cccggcgcct cgccgcgtaa cgcgaatggg acgatggccg
     5701 tgcatggccc ggcggcgccc gatgcggcgc tgccgatcga aggcgatatt ccatccttga
     5761 gcggcgcaac ggcctggttg aattccgggc ctctcacacc ggcggacttg cgcggcaagg
     5821 tggtgttggt cgatttttgg acgtattcct gcatcaactg cctgcgcgcc ctgccctacg
     5881 tatcgggatg gtacgaaaag taccaggctc atggtctcat cgtcatcggc gtgcatgcgc
     5941 cggaattcgc cttcgaaaaa aatgtcgaca acgtgcgccg tgccgtcgcg gatttgcaca
     6001 tcacttatcc cgtggcgctc gacaacgact atgcgatctg gcaaggattc aacaaccaat
     6061 actggccggc gcattatttc atcgactcgc agggacatat tcgcgctcat cattttggtg
     6121 aaggcagcta cgatgaatcc gaggccaccc tgcggcaatt gttgagcgaa gccggttaca
     6181 cggatttgcc gcccgccggc atgcaatccg cgatggccgt cggggcacag gcggcggcgg
     6241 acccagcgca catgcaatcg cccgaaacct acatcggtta ccggcgcgcc gaaaatttcg
     6301 cctcgcccgg tggatttgcg caagatcgcc ccaagaatta tgccgagcct gccgaattga
     6361 ggttgaatca gtgggctctg gcaggaccgt ggttggtgga tgcggaaaag gcgcagctgc
     6421 gcggggtatc cggcaagatc gagtttcgat tcttcgcgcg cgatctacat ctggtcgcgg
     6481 gcccaggcgg cgacggcaag cgggttcgat ttcgcgtgct gctcgatggc gccgcgcctc
     6541 tcgccaatca cggcgcggat accgacgaga acggcgaggg cgtgatcgac cagcagagac
     6601 tgtatcaact gatccgacaa tcgggcgctg tcggcgaaca tgttttcacc atcgaattcc
     6661 tggacgacgg cgtgcaggcg tattcgttca cttttggctg aggatcccca tgtcattcaa
     6721 gcatcgaccc aatcgcagcg ccgctgcgct cagccggcgt acctttatcg caggcctggg
     6781 cgcggcggcg ctcgcgacct acggttgcaa accgtcggcg gccactctct cggcacccga
     6841 gcagagcgcg gacgtgacga tcgaggagtt ttccgcggcc ggaaaaagcc ttggacacgt
     6901 gcgcgtcgcg aaggtcgtga agagcgcagc gcagtggcgc gagcaactgt cgccgctcgc
     6961 cttcagcgtc acgcgcgagc aaggcaccga gattgcattt accggcgagt acgacggcaa
     7021 tcatgccgac gggctgtatc gctgcgtgtg ctgcgatacg gcgctgtacg attcgaagac
     7081 gaagttcgac tcggggaccg gttggccgag tttctggcag ccgatctcgg ccttgaatgt
     7141 cgtcaagaat gccgacaaca gcctggggat gcagcgcgat gagatctcat gccgccgctg
     7201 cgctgcgcat ctcggccacg tgttcgacga cggtccgcgg cccacgggct tgcgctattg
     7261 tatgaattcg gttgccttgc attttgtcgc gcgcgcctga tgcgcaagga gcaatgatgt
     7321 cattctggaa gtatcgaacg tccctggggc tcggcgcgat gttgctgctc gcggcgctgt
     7381 ggggttcttg gcgctcgccg ctgttcggcg ccgaggcggc cgtcgccatc gcgccgccgg
     7441 cggtcgataa tccgaggact gcgggtccgg cgcagaccgc ggtgctggcc ggcggctgct
     7501 tttggggcgt gcaaggggtt ttcgagcacg ttcgcggcgt gcagaaggtg gtggcgggct
     7561 acgccggcgg cgagcgctcg accgcacaat acgagacggt gagttcgggt accaccggtc
     7621 atgcggagtc ggtcaagata accttcgacc cggcacagat ttcctacgga caaatattac
     7681 aaattgcgtt ttccgtggtg cacgatccaa cgcaactcaa tcgccagggc cctgatgtgg
     7741 gtacacagta ccgttcggcg gtgttctacg tggacgatgc ccaaaagcac atcgcgcaag
     7801 cctacatcgg ccagttggac aaatcgcatg cgttcccgcg gccgatcgtc actcgcgtgg
     7861 atccgttgca gggattttat gcggcggagg attatcatca ggattacctg atccacaatc
     7921 cggacgcgcc gtacattgcc tactatgaca ttcccaagat cgagaatttc aagcaagcgt
     7981 tcccgcagct ttacagcggg cggcccgtgt tggcgcacaa ctgagggtcc ttgatgaaac
     8041 gatttgccgg cgtttggctg acgtgctgcg tgtcgctgtg ggctcaagcg gccttcgcag
     8101 ccgccgcgga caagccgccg ctcacggtgt tcgcggccgc ttccctgacc aacgtgctgc
     8161 aggagctggg cgacggcttt accaaagaga cgcagattcc gatccgattt tcctttgcgg
     8221 cgagctcggc gctggcacga cagatcgaga atggttcgcc ggccgatata ttcttctcgg
     8281 ccgacttgga gtggatggat tacttgcaga cacgcggtct catccagcgt gactcgcggc
     8341 acaatctgct cggtaatcgc ctggtgctga ttgcgcccac cgagagcgcg atcaaattga
     8401 agatcgaacc tcactttgca ctcgccgcgg ccctcggctc cgggcacctc gcaaccggcg
     8461 atccggattc ggtgccggtc ggccgctatg cgcgcgaagc gctgacttcg ctcggcgtgt
     8521 ggaatgacgt ggcggatcgc gtggtgcgcg ccgacagcgt gcgttcggcg cttgcgttcg
     8581 tcgatcgaag cgaggcgccg ctcggcatcg tctacgcgac cgacgccttg atcgacaagc
     8641 gggtgcgcgt ggtggatgtc ttccccgaca acagccattt gccgattctg tatccgatcg
     8701 cgttgacccg tgccgcgaaa cccgacgcgg caaaattcgt cagctacctg agcggacccg
     8761 ccggaggcgt cgcattcaag gcctacggct ttacgccgcc gcgttaaggt cggccatgga
     8821 gcgatacgag agatgccatg agtgaaactg ccgccgtcga tggactgaaa atactgccga
     8881 cctcgcacag cgtcgcggag gtgctgcaac gggtccaggc atcggcgcgc gagcgcggcc
     8941 tcacaatctt cgcgcaaatc gatttcagcg gcgacgcgca acgttccggc ctcacgctgc
     9001 cgccggcggg catgattatc ctgggcaatc ctcgggcggg cacgccgctc atcgccgcga
     9061 cgccgacggt ggccatcgac ttgccgctga agattctcgc gtggcaggat gcgaacggca
     9121 agacttgggt cggctacaac gcggccgagt accttcaggc tcgacacggc ttcccatccg
     9181 cgctgctgaa gaacatcgct gccttggacg ctttggccgc cgccgcggct gcgtgagccg
     9241 gcgcgcagcg cacgtcaggg aatttccgga acgccgcggc ggatgtccgc ggagaaggtg
     9301 gcgctgcgat ccgcttgttt ggcgcggtcg gtaatgcggc caagcgcgcg cagctgctcg
     9361 agcgaatagc gcgaatcgag accgccgact tcatacacat agccgggaag atagcctgag
     9421 aagagcaggc gatagctcaa cggcaggtag ccgacgatgc gcttcatcat gtggtagacg
     9481 agagtcgtac agttgatcgt gatcgtgttg tagaagcgcg gcgtgtgcag cagcgaattg
     9541 gcctgatcga tgtaacccaa gaacagcgaa cgcatcgccg cgggcgacag gtgcagccga
     9601 tagagatagt cgtcctcgcc gcgaacattg gtgcgtagac ggaccacgtc gcgctcgtcg
     9661 gcggcgatga tactgagttc gaattccttg aagaagccgc cgatctcgac caggccttgc
     9721 aggccatcgg ttcacgggtc tgacggaaaa caatgtcaat caacatggca ttcgtgctgc
     9781 gaatcgtggc catctcgctg gcgacgttca tcgtcacgat ttcggcgctt tggggcgc
//
LOCUS       DASWZT010000522        10325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_47829, whole genome shotgun sequence.
ACCESSION   DASWZT010000522 DASWZT010000000
VERSION     DASWZT010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10325
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47829"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..816)
                     /locus_tag="VGE92_14950"
     CDS             complement(43..816)
                     /locus_tag="VGE92_14950"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HEY1315177.1"
                     /translation="MSQAQAISQPIRRPIGRIIAFSYGLLSYAVFFVAFLYAVGFVSG
                     LVVPKTIDTGAAAPFAQALLIDLLLMSAFALQHSVMARQQFKQWWTQYVPKSIERSTY
                     VLFASLVLLLLIWQWRPMPAIVWHIAAPDAARLLLGIGFAGWLIVLSSTFLINHFELF
                     GLHQVTNNLTGQPMPTPRFRTPLYYQFVRHPIYLGFIIAFWAAPTMTVGHLVFAAVTT
                     AYILVGILLEERDLIDLFGDDYRRYKQRVAMLVPWRKSA"
     gene            975..2552
                     /locus_tag="VGE92_14955"
     CDS             975..2552
                     /locus_tag="VGE92_14955"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     tetratricopeptide repeat protein"
                     /protein_id="HEY1315178.1"
                     /translation="MFFLFDDHSLDTDRRELRRGPESIAVEPQVFDLLVYLLQNRERV
                     LSKEDLIASVWAGRVVSDSTLTSRINAARRAIGDNGEEQKLIRTIARKGFRFVGEVRT
                     QAHAGEPTRALEPPLEQLRDPARASVPLSDRPAIAVLPFTNMSGDPQQDYFSDGISED
                     IITALSKLRWFFVIARNSSFVYKGRAVHMKQVADELGVGYVVEGSVRKSGDRVRITAQ
                     LNDAATGSHVWAERYDRSLADVFAVQDEITEAIVAAIEPQIYAAENFRAARKAPESLD
                     AWDLVMRAFSYFWRVTRQDTVVAQALLEKAIAIDPNYGQALGVLAATHSFSAHMGWEE
                     LTAAAPVAERAAMAALRADSEDPWAHLALGSVYLYARRFDDSLAEFELALRLNPNFSL
                     AQGYYGLALSYCGRWQEGDLAAHRALRLSPRDPYAAIYCGIASYCRFVGGDYEEALRL
                     SRESIRHRADFSGGHRVLTAAASMLGQTEVAAEALRTLRQVQPNVSLAWIATHLPIKL
                     DADREHYSEALRRAGLD"
     gene            2614..4749
                     /locus_tag="VGE92_14960"
     CDS             2614..4749
                     /locus_tag="VGE92_14960"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY1315179.1"
                     /translation="MHTLYRKLTAASRTVTKFWSSLFAGLAWPYIDFAIRVWLAKLFF
                     SFGVLQLMHWQPTLALASQENPVPLLAPVAAAIFSTGVYLLCAALLALGLMTRYAALP
                     LLALSCVTQLLYQPFDTQLFWIALSSWFVIHGAGPIALDQLLRRGLGDSALPFVPAMI
                     KLSTWLRAHGTPLYVSLLRIWLGTAMLVAAYASVHRMDLETIGRWLPLDVATRIPLTA
                     AWVGGTLLVIGAGTRYIATVLFVSLVAYSVVDPRATDSIYLLMCLAILGIYGGGHLSL
                     DRTVLRWRERFSPVNRSPGLTVDGLPRVVIVGAGFGGLRCAQALRDVAVAVTLIDRTN
                     YHLFQPLLYQVATAALSPGDIATPARQMFRNSPATRLLLGTVTGVDTTRRMVSMQDGE
                     IPYDYLVLATGATHSYFGKDHWSPFAPGLKRIEDALEIRRRILTAFERAEATSSEAER
                     NALLTFLIVGGGPTGVELAGAIAELARYGLDNDFRAFDPSKARVILVQAAPRLLPSFP
                     EGLANIAQRSLEQLGVQVHLGGRVDHIDAAGVAVNGTRIEARTVLWAAGVSASPAARW
                     LNVPADKAGRLIVDEGLRVPGLAGVFAIGDTVASQAWNGELVPGLAPAAKQGGTYVAK
                     QIRALIDAKTPLPPFRYRHYGSLATIGRKAAVADFGIFKLSGAPAWWLWGVVHVGFML
                     DVRNRVATLTNWFWAYLRFGGSIRLITGS"
     gene            complement(4746..5690)
                     /locus_tag="VGE92_14965"
     CDS             complement(4746..5690)
                     /locus_tag="VGE92_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315180.1"
                     /translation="MGVVLIVSNTGDIHCDFLVDACARQPVECFRLNTDQFRKSGLLD
                     WRIDSGDGLIKIGERECRLLDVSLLIYRRPIPVHQLRTDIEPWIRRLLDSEWSAIEWA
                     LSRAVNCRVMNGLAGSALAQNKILQLKAARDAGLNVPETLISTDAHALRTFAREQRCI
                     TKGIVNAFHLDGKTLRSAFTSFVAPGTLDNFDPTGCPTLLQRAIESAAIWRIVMVGGK
                     ALGFRFHGGGLKQVADSRPIERALEGAYQDVPAAVAANLAAMCSALSIEFASADFVED
                     ALGQLWFLDLNPEGQWAFLEDRFGVRISDEIVSLARRA"
     gene            complement(5742..5987)
                     /locus_tag="VGE92_14970"
     CDS             complement(5742..5987)
                     /locus_tag="VGE92_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315181.1"
                     /translation="MDSSSKESVRPVKGLPLFMVNHTITDSSQVFPRAGVDFVYDDSA
                     QKAVWSNPDSTNKWTPTAYNDTQVDGVDYIAANFIAE"
     gene            6213..8792
                     /locus_tag="VGE92_14975"
     CDS             6213..8792
                     /locus_tag="VGE92_14975"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY1315182.1"
                     /translation="MPFYPNLELVTLRLAGLEGVERAFVLFSDDHCLWLDGSSAPIHK
                     ANYLESIVVTAAQVLDYVRFFCFFVQGTDGSFNLLEAPDSLTATTATAPQLEAARKVI
                     VERPFAAVEEEGGFRVTAAMAYRQTLFETTLVVARDGDVQMVDDTSLLSLDGFDYPDG
                     PQLAEEPGDPIPDVSSPTAPIEPRSRSDRDATEAMVSVLLNEATAAKLNHTLLQRFNS
                     KSEAAGAIATLVRFVTEFTPVIIIESDIPFIEDIAAGIINANGKLFSRAEIERAHAAS
                     HDDGCCTVALGDSKKLRLISFHAYRKLWDAEWTAHLLGMDSAVVLIGCEQVKDVPGAL
                     QQVADLVLTLPHVDGKLFPRIFKRVFGVAAPPGWDAPNADWPRYLQHTDFHAPLRLGL
                     SIEETIVYLRGRSHERLAEVSPGESPRLDELHGMGEAREVAEDLIADVAAARSGKIPW
                     SAVDRGLLLVGAPGTGKTTLVKAIAQACDVKFVQASATQWQSMGSLDAHLRAMRDSFN
                     EARRFAPAILFIDEIDSIGNRELLTGHNAGYFTDIINALLEQMQGMNPEEPVIVIAAT
                     NHVSKVDPALRRAGRLDQTISIPLPSVAALEQIFGFYLKPYRADALIGEDIDEHLLGQ
                     LAFGLTGADVEFFVRGAARRARKEGRKISQADLTAAVTRRPRQRNGLFRLSADDMRRV
                     AVHEAGHALCAYLGDKGNAPPTYVSIIPRSDGSLGFTATIPSDSMLLTRAQLDARLRT
                     MLAGRAAEELVFGADGVSSGAGGAETSDLAVATRLATYVVCTSGLGPSHGLYWTTAAS
                     PKQQHEAEQLLADAYRATLGILSANRAALERISAGLVAEQELTGQRVKELINVAPSPA
                     GAR"
     gene            complement(8846..9082)
                     /locus_tag="VGE92_14980"
     CDS             complement(8846..9082)
                     /locus_tag="VGE92_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315183.1"
                     /translation="MPGGTVGLIFGVFGGAGRGGVDGNSAGFGVDGEVGGESQSWHIH
                     DVPLLGVVSSMPARRCIWQTRILGIFVGQQVLRP"
     gene            complement(9159..9608)
                     /locus_tag="VGE92_14985"
     CDS             complement(9159..9608)
                     /locus_tag="VGE92_14985"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY1315184.1"
                     /translation="MNTRNEPWSVLSQLQRHLNEYFDPDGANHANGSSAATADWIPPA
                     DVEEYQDRFVLKIDIPGVDPNAVDITLEQGILSVSGNRAKERTENDAVQRERSERVSG
                     SFHRRFALPETADANGVQAAGKNGVLQIVIPKQPKAQPKRIKVALEN"
     gene            complement(9730..10182)
                     /locus_tag="VGE92_14990"
     CDS             complement(9730..10182)
                     /locus_tag="VGE92_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315185.1"
                     /translation="MSEADLSILRAALEEPNSEVPILMTTTPGSKNDLLWTEMVALGW
                     MSHAEPLDVAVSSKVFRINPAAKESIAGFVAGRSHSAAMTKLINDLRADIAPKLIEAV
                     HRVDGTPADLAIMLAAVVESTMRRAIKPELHDEFLREVAKVAEAMRSN"
BASE COUNT         1842 a         3235 c         3226 g         2022 t
ORIGIN      
        1 ccccggattt cgtttcactc aatccggcta cacttgctcc gatcaggccg acttgcgcca
       61 aggcacgagc atcgcaacac gctgtttgta gcggcgatag tcatcgccga acaagtcgat
      121 gaggtcccgc tcctccaaca aaatccccac gaggatgtaa gccgtcgtga ccgcggcgaa
      181 caccaggtga cccaccgtca tggtcggcgc tgcccaaaac gcgatgatga aaccgaggta
      241 gatcggatgt cgcacgaact ggtagtacag cggcgtgcgg aagcgcggcg tcggcatcgg
      301 ttggcctgtg agattatttg tgacctggtg caggccgaac agctcgaagt ggttgatcag
      361 aaacgtgctg ctcaacacga tcaaccagcc tgcaaaccca atgcccagca gcagccgcgc
      421 cgcgtcggga gccgcgatgt gccagacgat cgcaggcatc ggccgccact gccagatcag
      481 aagcaataga accaggctcg cgaacaggac ataggtactg cgttcgatcg attttggcac
      541 atattgtgtc caccattgct tgaactgctg gcgcgccatg acactgtgct gaagggcgaa
      601 tgcggacatc aacaacaggt cgatgagcag cgcttgcgcg aacggtgcgg cggcgcccgt
      661 gtcgatggtt ttcggcacca ccaggcccga aacgaagccg accgcgtata gaaaggccac
      721 gaaaaatacg gcgtaagata gcagaccgta cgaaaatgcg atgatccggc cgatgggccg
      781 gcggatgggt tgactgatcg cttgcgcttg gctcatggac attccccgtg ttcttcggat
      841 cgcgggaatc gcgattcatt ggcgcttaga ctagcggggg caccgccaag agaccttgcc
      901 ggcggcttga tttgtccttg aggccagcct gattatttcg tgagaacgac cgcgcagccg
      961 ggtcgggggg ctacgtgttt tttctgttcg acgaccattc gctcgatacc gaccggcggg
     1021 aactgcgccg ggggccggaa tccattgccg ttgaaccgca ggttttcgac ctcctggtct
     1081 acctgctgca gaaccgggag cgcgtcttga gcaaggaaga tctgatcgcc tccgtctggg
     1141 ccgggcgggt cgtgtcggat tcgaccttga ccagccgcat caacgccgca cgacgcgcaa
     1201 tcggcgacaa cggcgaggag cagaagctga tccgtaccat tgcacgcaag ggttttcgct
     1261 tcgtcggcga ggtacgcacc caagcccatg ccggcgagcc gacccgcgcg ctcgagccgc
     1321 cgctcgagca actccgcgac cccgcgcgcg catccgtgcc gctgtccgac cgaccggcca
     1381 tcgccgtgct gccgttcacg aatatgagcg gcgatccgca acaggattat ttctcggacg
     1441 gcatcagcga ggacatcatc accgcgttgt cgaagttgcg ctggttcttc gtgatcgcac
     1501 gcaattcctc cttcgtctac aagggcaggg cggttcacat gaaacaggtt gcggatgaac
     1561 tcggcgtggg ctacgtcgtc gagggaagcg ttcgcaagag cggcgaccgg gtgcgaatca
     1621 ccgcgcagct caacgatgcc gccaccggca gccacgtgtg ggcggagcgc tacgaccgca
     1681 gtctcgccga tgtatttgcg gtgcaggatg agatcacgga ggccatcgtc gccgcgatcg
     1741 aaccgcagat ctacgccgcc gagaattttc gcgccgcgcg caaggcgccg gaaagcctgg
     1801 acgcctggga tctggtgatg cgtgcatttt cctacttctg gcgcgtcaca cggcaggaca
     1861 ccgtggttgc gcaggcgctg ctcgagaagg ccatcgcgat cgatccgaac tacggtcagg
     1921 cgctcggagt gttggccgcg acccactcct tcagcgcgca catgggctgg gaggaactga
     1981 cggcggcagc gcccgtcgcc gagcgcgccg cgatggcggc gctgcgcgcc gacagcgagg
     2041 acccgtgggc gcatctcgcg ctcggcagcg tctatcttta cgcacggcgc ttcgatgact
     2101 ccctggccga attcgagttg gcgctgcggc tgaatcctaa tttttcgctg gctcaaggct
     2161 attacggcct ggcgctgtcg tattgcggcc gttggcagga gggcgacctg gccgctcacc
     2221 gcgccttgcg cttgagtccc cgcgatccgt atgccgccat ctattgcggc atcgcctcct
     2281 attgccgatt cgtcggaggc gattacgagg aggccctgcg cctgtcgcgg gagtcgatcc
     2341 ggcaccgcgc ggatttctcc ggcggtcacc gcgtcttgac tgcggccgcg agcatgctgg
     2401 ggcagacgga agtcgctgcc gaagcgctga gaacgctgcg ccaagttcag cccaatgtat
     2461 cgctcgcctg gattgcgacg catctgccga tcaaactgga cgcggatcgg gagcactatt
     2521 cggaggcgct gcggcgcgcg ggtcttgact gaaggcgcag gcggagaacg cttgtcgttg
     2581 caataaagcg tgtcggactg cggtcttctt ggcatgcaca cgctctacag gaagctcacc
     2641 gccgcatcgc gcacggtcac gaaattctgg tcttccctgt tcgcggggct tgcgtggccg
     2701 tacattgact tcgcgatccg cgtgtggctt gccaaactct tcttctcgtt cggcgtgctg
     2761 cagctcatgc attggcagcc gacactcgcg ctcgcgagcc aggagaatcc ggttccgttg
     2821 ctggcgccgg ttgcggcggc gatcttcagt accggggtct acctcctctg cgccgcgctt
     2881 ctcgcgctcg gtctgatgac tcggtatgcg gcgctgccgc tgctggcgct gtcctgcgtc
     2941 acccagcttc tatatcagcc gttcgatacg caattatttt ggatagccct gtcgagttgg
     3001 ttcgtgatcc atggcgcagg cccgatcgca ctcgatcagt tgttgcgccg cggccttggc
     3061 gacagcgcgc tgccgtttgt tccggcgatg atcaagttgt cgacgtggct aagagcccat
     3121 ggcacgccgc tctatgtctc cttgttgcgc atctggctgg gtacggcgat gctggtggcg
     3181 gcgtatgcca gtgtccaccg aatggatctt gagacgatcg ggcgctggtt gccgctcgat
     3241 gtagccacgc gaatcccgct caccgcggcg tgggtgggcg gcacgctgct cgtgatcggc
     3301 gcaggcacgc gctacatcgc gacggtattg ttcgtgagcc tcgttgccta ttcggtggtc
     3361 gatccgcgcg cgacggattc catttacctg ctcatgtgtc tcgcgattct cgggatttac
     3421 ggcggcggac acctgtcgct cgatcgcacg gtgctgcgct ggcgcgagcg cttctccccg
     3481 gtcaatcgat cgcccggcct gacggttgac gggctcccgc gggtcgtcat cgtcggtgcg
     3541 ggatttggcg ggctgcgatg cgcacaggcg ctgcgcgacg tggcggttgc cgtgacgctg
     3601 atcgatcgca ccaattatca tcttttccag cccctgcttt accaggtcgc gacagccgcg
     3661 ctatcgccgg gcgacattgc gaccccggcg cggcagatgt ttcgaaactc cccggcgacg
     3721 cggctgttgc tcggcaccgt caccggcgtc gacacgacgc gccgcatggt gtcgatgcaa
     3781 gacggtgaga ttccctacga ctatttggtt ttggcgaccg gcgccactca tagctacttc
     3841 ggtaaagacc actggtcgcc gttcgccccg gggctcaagc ggatcgaaga cgccttggag
     3901 attcggcgtc gaattctgac cgctttcgag cgcgccgagg ccacgtcgag cgaagcggag
     3961 cgcaacgccc tgttgacgtt cctgatcgtc ggcggtggcc ccaccggcgt tgaactcgcg
     4021 ggcgcgatcg ccgaattggc gcgctatgga ctggacaacg acttccgcgc attcgatcct
     4081 tccaaggcgc gcgtcatcct ggttcaagcc gcgccgcgcc tgttgccgag ctttcccgaa
     4141 gggctcgcga acatcgccca gcgctcgctc gaacagctcg gggtgcaagt ccacctcggc
     4201 ggccgagtcg atcacatcga tgcagcgggc gttgcggtca acgggacgcg catcgaggcg
     4261 agaacggtgc tctgggcagc gggggtctcg gcctcaccgg ccgcacggtg gttgaacgta
     4321 cccgccgata aagcgggccg cttgatcgtg gacgaggggc tgcgagtgcc aggactcgcg
     4381 ggagtcttcg cgattggcga taccgtcgcg agtcaagcct ggaacgggga gttggtgccg
     4441 ggtctcgcgc ccgcagccaa gcagggaggc acgtatgtcg ccaaacaaat tcgcgcgctc
     4501 atcgatgcga aaaccccgtt gccgccgttt cgctaccggc actacggcag cctcgcgacc
     4561 atcggccgca aagccgcagt cgccgatttt ggcatcttca aactgtcggg ggcgcccgct
     4621 tggtggctct ggggcgtggt gcacgtggga ttcatgttgg acgtcagaaa tcgcgttgcg
     4681 actctcacca attggttttg ggcctatttg cgatttggcg gcagtattcg gctcatcacc
     4741 gggagctagg cgcgccgcgc caagctcacg atctcatcgg aaatgcgcac gccgaaccgg
     4801 tcctccaaaa aagcccactg cccttccggg ttcaagtcca agaaccacaa ctgcccgagc
     4861 gcgtcttcca cgaagtctgc ggacgcgaat tcgatcgaca gggcgctgca catggcggcg
     4921 agattggccg caaccgccgc cggcacgtct tggtaagccc cctcgagcgc gcgctcgatg
     4981 gggcgtgaat ccgcgacttg cttgagtcct ccgccgtgaa aacgaaatcc caaggccttg
     5041 ccgcccacca tcacgatgcg ccagatggcg gctgactcaa tggctctttg cagcagtgtc
     5101 gggcagccgg tcggatcgaa gttgtcgagc gttcccggcg cgacgaagga ggtgaaggcc
     5161 gagcgcaaag tcttgccgtc caagtggaag gcattgacga tacccttggt gatgcaacgt
     5221 tgctcgcgcg caaaggtgcg caacgcgtgc gcgtcggtgc tgatgagagt ttcgggaacg
     5281 ttcaagcccg catcgcgcgc ggccttcagt tgcaaaatct tgttttgggc gagagccgaa
     5341 cccgcgagtc cattcatgac gcggcaattg accgcccgcg acagtgccca ctcgatcgca
     5401 ctccactcgg aatcaaggag ccggcgtatc cacggctcga tgtccgtgcg cagttggtgg
     5461 acgggaatgg gtcttcgata aatcaacaga ctgacgtcga gcaaacggca ctcccgttcg
     5521 ccgatcttga tgaggccgtc gccgctatcg atgcgccaat cgagcaagcc cgactttcgg
     5581 aactggtccg tgttcagccg gaagcactcc acaggctgtc gggcgcatgc atcgacgagg
     5641 aagtcgcaat gaatgtcgcc ggtgttcgag acaattaaaa cgacacccac ggtattgatt
     5701 cgcgcgacgc tgcgccacgc ccgtggtgca gttgaactcg gttattcggc tataaagttg
     5761 gccgcgatat agtcgacccc gtcaacctgg gtgtcgttat aggcagtggg agtccacttg
     5821 ttcgtggagt cgggattcga ccaaaccgcc ttctgggcgc tatcgtcata aacgaaatca
     5881 actccggcgc gcggaaatac ctggctgctg tcggtgatgg tgtgattgac catgaatagc
     5941 ggcaggcctt tgaccggacg aacactttct ttggatgaac tgtccatgag aactcctgac
     6001 tatccacgca tttaaatagt ttatgctttg gtctcgccac ccgcaacccg acgggaacag
     6061 cgcggtacgc ccttatggag caaagcctcg gcccgaatat cgcgtggacg ccatgcgacc
     6121 agagcgagct gcgccgcatt gcggattcgc ttcgctcact ggcgccggcg acttttcata
     6181 atgtcgaact gagcgactgt cgccgggcgc ctttgccgtt ctatccaaac ctcgagttgg
     6241 tgaccctgcg tctcgccggt ctcgagggcg tcgagcgcgc cttcgtgctg ttcagtgacg
     6301 atcattgcct gtggctcgac ggctcgtcgg ccccgataca taaggcgaac tatctcgaat
     6361 cgatcgttgt cacggccgcc caggttcttg attacgtgcg gttcttctgc tttttcgtgc
     6421 aaggcaccga cggctcgttc aatctgctcg aggcgccgga cagcttgacg gcaaccaccg
     6481 cgaccgctcc gcagctcgag gcggcccgca aggtcatcgt cgaacgcccg ttcgcggcgg
     6541 tcgaggaaga gggcggattt cgggtgaccg ccgcgatggc ttatcgccaa actctgttcg
     6601 agaccacgtt ggtggtggca agggacggcg atgttcaaat ggtggacgac accagcctgc
     6661 tgtcgctcga cggattcgac tatcccgacg gaccgcagct cgcggaggag cccggcgacc
     6721 cgatcccgga tgtgtcctcg ccgacggcgc cgatcgaacc ccggtcgcgc agcgatcggg
     6781 atgcgaccga ggcgatggtg tccgtgctgc tgaacgaggc cacggcggcc aagctcaatc
     6841 acactttatt gcagcgattc aattcgaagt cggaggctgc cggcgcaatc gcgaccctgg
     6901 ttcgtttcgt cacggaattc acgccggtta tcatcatcga gtcggacatt ccgttcatcg
     6961 aagacatcgc agccggcatc atcaatgcga atggcaagct attttcgcgc gccgagatcg
     7021 aacgcgctca cgctgccagt cacgacgacg ggtgttgcac cgtggcgctc ggcgattcga
     7081 agaaactgcg cctcatctct tttcacgcgt accgcaaatt gtgggatgcg gagtggaccg
     7141 cgcatctgct cgggatggat tcagccgtgg ttctgatcgg ctgcgaacag gtgaaagacg
     7201 taccgggcgc cctgcagcag gtcgcggatc ttgtgctcac gctgccgcac gtcgatggga
     7261 agctgttccc gcggatattc aagcgggtgt tcggcgtcgc ggcgcctccg gggtgggatg
     7321 cgcccaacgc ggactggccg cgctacctgc agcacacgga ttttcatgca ccccttcgct
     7381 tgggcctctc gatcgaggag accatcgtct atctgcgtgg gcgcagccac gagcgcttgg
     7441 ctgaggtgtc gcccggcgaa tcgccgcgtc tcgatgaact gcatggaatg ggcgaagctc
     7501 gcgaggttgc cgaagacctg atcgccgacg tggcggccgc acgcagcggg aagattccct
     7561 ggtccgccgt cgatcgcgga ttgctgttgg ttggagcgcc gggcacgggc aaaacgacgt
     7621 tggtgaaggc catcgcgcag gcatgtgacg tgaagttcgt gcaggcgagc gcgactcaat
     7681 ggcagagcat ggggtcgctc gatgcgcatc tgcgcgccat gcgcgacagt ttcaatgaag
     7741 cgcgccgctt tgcacccgcc attctgttca tcgatgaaat cgacagcatc ggcaaccgcg
     7801 aactcctgac gggccacaat gccggctact tcaccgacat catcaatgcc ctgctcgagc
     7861 agatgcaggg gatgaatccc gaagagccgg tcatcgtcat tgcggccacc aatcatgtgt
     7921 cgaaggtcga tccggcgcta cgccgcgccg gccggctcga ccagacgatc agcatcccgc
     7981 tgcccagcgt cgcggcactt gagcagattt tcgggttcta tctcaagccg tatcgcgcgg
     8041 atgcgcttat cggcgaggac atcgatgaac acctgctggg acaactcgct ttcgggctga
     8101 ccggcgccga cgtggagttc ttcgtacgcg gcgcagcgcg gcgcgcgcgc aaggaaggtc
     8161 gcaagatttc gcaggccgat ttgacggcgg ccgtcacgcg gcggccacga cagcgcaatg
     8221 gcctgttccg tttgagtgct gatgacatgc ggcgggtcgc cgttcacgag gccggccacg
     8281 cgctgtgcgc ctacctcggc gataaaggca atgcgccacc cacctacgtc tcgatcatcc
     8341 cgcgttccga cggttctctc ggcttcaccg ccactatccc gagcgacagc atgctgctca
     8401 cccgcgcaca gctcgacgcg cggctgcgca ccatgctcgc ggggcgagcg gccgaagaac
     8461 tcgtgtttgg cgccgatggt gtcagcagcg gcgcgggcgg cgctgaaacc agtgatctcg
     8521 cggtggccac ccggctcgcg acttacgtcg tttgtacctc gggcttaggt cccagtcacg
     8581 gcctgtactg gaccacggcg gcgtcgccca agcagcagca tgaagcggag cagctgctcg
     8641 ccgatgccta tcgcgcgaca ctcggaatcc tgagcgcgaa ccgggctgcg ctcgagcgca
     8701 tcagcgccgg attggtggcc gaacaggagc tcaccgggca acgagtcaag gaactcatca
     8761 acgtagcacc gtccccggcc ggcgcgcgtt gagcccgtgg gcccggccat gcttgcggcg
     8821 tcgtgcggca acgcttcatc tttccctaag gccgcaatac ctgttgtcct acgaaaatcc
     8881 ccaagattcg cgtctgccaa atgcaccgtc gagcgggcat ggaactcacg acgcccaaga
     8941 gggggacatc atgaatgtgc cacgattgac tctcgccgcc gacttcgccg tcgacgccga
     9001 acccggcgga attgccgtca acaccgcccc gtcctgcacc gccaaacacg ccgaatatca
     9061 atccgactgt tccgccgggc acgactccgc caccgacgac ccagccgccg tagcagagtt
     9121 aagtttccgg gggtgcgccc cccggaaact cgcggatctt agttttccag cgcgaccttg
     9181 attcgcttcg gctgggcttt cggctgcttc ggaatcacga tctgcagcac gccgttcttg
     9241 ccggccgcct ggacgccatt cgcgtctgcc gtttccggca acgcaaaacg gcgatgaaaa
     9301 ctcccgctga cccgctccga gcgttcgcgc tggacggcat cgttctccgt gcgctccttg
     9361 gcgcgattgc cggaaaccga caggatcccc tgttcgagcg tgatgtcgac tgcgttcggg
     9421 tcgacaccgg gaatgtcgat tttgagtaca aagcggtcct ggtattcctc cacatcggcg
     9481 ggcggtatcc agtcggccgt ggccgcggaa ctgccgttcg catggtttgc gccatcaggg
     9541 tcgaaatatt cattgaggtg cctttgcagc tggctgagta cggaccaggg ctcgttacgc
     9601 gtgttcatgg ttcaacctcc tgtttgggtt aagggttacc gttcccggct gctatagggc
     9661 gtggccgcaa tttttcaagg ggcgcccggt ggggtgtgcg ctgcatcaag cgccgcgatc
     9721 gcgttccctt tagttcgagc gcatcgcttc ggccaccttg gcgacctcgc gcagaaattc
     9781 gtcgtgaagc tcgggtttga ttgcgcgccg catggtgctc tcgacgaccg cggcgagcat
     9841 gatcgcgagg tcagccggcg tgccatcgac gcggtgcacc gcttctatca acttcggggc
     9901 aatatcagcc cgcagatcgt tgatgagctt ggtcatcgcc gcggagtggc tgcgcccggc
     9961 cacaaatccc gcaattgatt ccttcgccgc cggattgatg cgaaacacct tggaactcac
    10021 cgcaacgtcg agaggctcgg cgtgactcat ccagccgagc gcgaccatct ccgtccacag
    10081 caaatcattc ttcgagcccg gcgtcgtcgt catcaggatc ggcacttcgg agttcggttc
    10141 ctccaaggct gctcgaagta tcgagaggtc tgcctcactc acggcgttgc ccagcattgt
    10201 caagtctggt ttttgctcgt tcactgcttt tgcctccggc gcgcgctcaa tgagcagccg
    10261 gtgcctcgac gggaacccgc ggcaaacatt cattacgctc cgccaggaaa taagcaagcg
    10321 tcgcc
//
LOCUS       DASWZT010000523         2451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_57455, whole genome shotgun sequence.
ACCESSION   DASWZT010000523 DASWZT010000000
VERSION     DASWZT010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57455"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VGE92_14995"
     CDS             <1..214
                     /locus_tag="VGE92_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315186.1"
                     /translation="DKQGNDRFTTEIIADNMQMLGGRAGGGGGGGAMGGGERGAGGGA
                     PPREDYDQSAPAPAGGKEDFDDDIPF"
     gene            216..1322
                     /locus_tag="VGE92_15000"
     CDS             216..1322
                     /locus_tag="VGE92_15000"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY1315187.1"
                     /translation="MRQATTLGSWVKSICRAFEAAGCDSAALLAEAGFGPKSLDNPTT
                     RYPLTQTARLWRIAVATTGDPAFGIKVASHIKPTTFHALSFGLSASSTLKEAFERAQR
                     FCHVGSDAIEYQFSRTGAEYHFVIEPAADVADESVDALVAAYVRMCRSMIGHDYSPLA
                     IEFRRASPARIEDFQSLLRAPLEFGAAQTRLKFDAESIERPLDGGNPELARQNDAIAL
                     QYLSQIERDNIQWRVRDVLMQRLAQGEPSQEEVAELLNVSARTLQRKLGEGGVTYKEI
                     LDETRRSLALAYLSASRHTVSDVTYLLGFSAASSFTRAFRRWTGQSPSDWRAQGSRAQ
                     VAARGAVPNDASSSLASAMSRGRTSTNRNSFDTM"
     gene            complement(1214..2011)
                     /locus_tag="VGE92_15005"
     CDS             complement(1214..2011)
                     /locus_tag="VGE92_15005"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY1315188.1"
                     /translation="MQRVIVKTDRHFVTALARGLEVLACFRRDRMLGNQELARRCGLA
                     KSTVSRLTHTLTALGYLVYVEDSAKYSLGTATLSLGSAMLSRLDIRKLAHPLMQQLAE
                     FGQCMVSLGSRDRLSMIYIDAVRGSAAVTLSLEAGARIQIANSAMGRAYLTAVPEHER
                     SDIMARVRELADVNRWPELQRGLDQALRDIRELGVCCSFGDWQKDVNAIAVPVRPGGG
                     LPPMAINCGAPAYMVSKEFLLVEVRPRLIALANELEASLGTAPRAAT"
     gene            2160..>2451
                     /locus_tag="VGE92_15010"
     CDS             2160..>2451
                     /locus_tag="VGE92_15010"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY1315189.1"
                     /translation="MRLEFTDEEQAFREEVRSFLHDQLSPEISAKVLNGYELGREDHL
                     EWQRRLHSRGWGGMGWPVEFGGPGWNSVQQYIFEEESALAGGPRLIPFGTKMV"
BASE COUNT          453 a          771 c          781 g          446 t
ORIGIN      
        1 ggataagcag ggcaacgatc gatttaccac cgagatcatc gccgacaata tgcaaatgct
       61 gggtggccgt gcaggcggtg gcggtggcgg gggagccatg ggcggcggcg agcgcggcgc
      121 cggcggtggc gctccgccgc gggaggatta cgatcaatcg gcgccggcac ccgccggcgg
      181 gaaagaggat ttcgacgacg atattccatt ctagtatgcg tcaggcaacg acgctcggca
      241 gctgggtcaa gtcgatttgc cgcgctttcg aggcggccgg atgcgacagt gcggcgctcc
      301 tcgccgaggc gggcttcggt cccaagtcgc tcgacaatcc tacgacgcgg tatccgctca
      361 cccagaccgc acgcttgtgg cgcatcgcag tcgccacgac cggcgatccg gccttcggca
      421 tcaaggtggc gagccacatc aagccgacca catttcacgc gctgagcttc ggcttgtcgg
      481 cgagttccac gctcaaggaa gcgttcgagc gtgcgcaacg cttttgccat gtggggagcg
      541 acgccatcga gtatcagttc tcacgcacgg gcgcggagta tcacttcgtc atcgagcccg
      601 cggccgacgt cgcggacgaa tcggtcgatg ccctggtggc agcctatgtg cgcatgtgcc
      661 gctcgatgat cgggcacgat tattcgccgc tcgccatcga gtttcgccgt gcatcgccgg
      721 ccaggatcga agattttcag agtctgctgc gggcgccatt ggaattcggc gcggcgcaga
      781 ctcggctcaa gttcgacgcc gagtcgatcg agcgaccgct cgatggcggc aacccggaat
      841 tggcgcggca aaacgatgcg attgcgcttc aatatttatc acagatcgaa cgcgacaaca
      901 ttcagtggcg ggttcgcgat gttttgatgc agcgcctggc ccaaggggag ccttcccaag
      961 aagaggtcgc cgagctgctc aacgtgagcg cgcgcaccct gcagcgaaag ttgggtgagg
     1021 gcggcgttac gtacaaggaa attctcgacg agacgcgccg ctcgctcgcg ttggcgtact
     1081 tgagcgcgtc gcggcacacc gtcagcgatg tcacctacct gttggggttc tccgccgcga
     1141 gttccttcac gcgtgcgttt cgacgctgga ccgggcagtc gccgtcggat tggcgggcgc
     1201 aaggctcgcg cgctcaagtc gccgctcgcg gcgcggtgcc caatgacgct tccagttcat
     1261 tggcgagcgc gatgagccgc ggccgcacct ccaccaacag gaactctttc gacaccatgt
     1321 aggcgggcgc gccgcaattg atcgccatcg gcggcaatcc gccgcccggc cgcaccggca
     1381 ccgcaatggc attgacatct ttctgccagt ctccaaacga gcagcacacg ccgagctcgc
     1441 ggatatcgcg cagcgcctgg tcgagaccgc gttgcaattc cggccagcgg ttgacgtcgg
     1501 ccaactcgcg cacccgagcc atgatgtcgc tgcgttcgtg ttccgggacg gcggtgagat
     1561 aggcgcggcc catcgctgaa ttcgcgatct gaatcctggc cccggcctcg aggctgagcg
     1621 tgacggccgc cgaaccgcgc accgcgtcga tgtagatcat cgacagccga tcgcggctgc
     1681 cgaggctcac catgcattgg ccgaactcgg ccaactgctg catcagggga tgtgccagct
     1741 tgcgtatgtc gaggcgcgac agcatggcgc tgcccaagct caaggtcgcg gtgcccagac
     1801 tgtatttcgc actgtcctcg acgtagacca gatagccgag cgcggtcagc gtgtgggtga
     1861 gcctcgacac cgtggatttg gccaggccgc agcggcgcgc cagctcttga ttgccgagca
     1921 ttcgatcacg gcgaaagcac gccaacactt ccaggccgcg cgccagcgcc gtcacgaagt
     1981 gacgatcggt tttgacaata acccgctgca tcggcccttt tccccgtttg cgtgcctgcg
     2041 ctttcagggc tctgttcatt gacaatctcc atcgcgcgta cacataattt tgcactaatt
     2101 cggaatttaa ttctgtactg cagaactcga gacccacccc atgatcagcg gcgacccgca
     2161 tgcgccttga atttaccgac gaagaacagg ccttcaggga agaggtgcgc agcttcctgc
     2221 acgaccagct gtcccccgag atcagcgcca aagtattgaa cggctacgaa ttaggccgcg
     2281 aagaccactt ggagtggcag cgccgcctgc acagtcgagg ctggggcgga atgggctggc
     2341 cggtcgaatt cggcggcccg ggctggaact ccgtgcagca atacattttc gaggaggaat
     2401 cggcgctggc aggcggtccg aggctgattc ccttcggcac caagatggtc g
//
LOCUS       DASWZT010000524         3209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_62220, whole genome shotgun sequence.
ACCESSION   DASWZT010000524 DASWZT010000000
VERSION     DASWZT010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62220"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VGE92_15015"
     CDS             complement(<1..463)
                     /locus_tag="VGE92_15015"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY1315190.1"
                     /translation="MDRDANYVAVGAFVLLVIAMAGSFVLWYTNNQDKAIYLTYEIYF
                     PGTVSGLTQGSPVRYLGVDVGKVTRILIDPQQRNRVLVIADIQATAPIDGSTRASLSL
                     QGVTGLLFIDLEQDHKAATPGVLADGLHYPVIRSAPSDFDVLLSSLPALTTH"
     gene            complement(456..1235)
                     /locus_tag="VGE92_15020"
     CDS             complement(456..1235)
                     /locus_tag="VGE92_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1315191.1"
                     /translation="MPEPTSVPVIRVRGLVNRFGAQVVHDGLDMEVRANEIFGIVGGS
                     GAGKSVLLRTILGLKEPDAGSVELYGRNVQQLAAADRSALVRSYGVTFQNGALISSLT
                     VAQNIQLPIREYYPASEKTLDELAELYLALVGLAADAAAKYPSQLSGGMIKRAALARA
                     LSLEPKLLLLDEPTAGLDPISAAAFDELLLYLHSQLRLTVVMITHDLDTLFRTCSRVG
                     VIVDGRMTTDSLDGMLHNQTPWIHDYFHGSRARAAAKDADG"
     gene            complement(1235..2368)
                     /locus_tag="VGE92_15025"
     CDS             complement(1235..2368)
                     /locus_tag="VGE92_15025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1315192.1"
                     /translation="MSQSMTVLELEGAGEDWHLHLNGDWSLASIAQVEVQLKSLPGTL
                     HGTLICDWSHAQLPGIGPVWALLMRLGEIGSQRLNVQHAGAPPHFLELLQKLKAQQHA
                     AQAAPYLHPGIETSVGNLGRWAVLQGSEARAVLGFFGRIVTVLGAAFSRPRALRLSSV
                     TRHIYETGITAIPIVSLIAFLISVIVAYLGAQQLSRFGADIFVVDLVTISVLREMGVL
                     LTAIIVAGRSGSAFAAEIGVMQLNEETDALRAMGMNPIELLVVPRLTALIIALPLLTV
                     IADAMGLAGGGLLSLLNLHIPLEQFTNRLRESLAPTTFWAGLIKAPVFAAVIAMVGCY
                     RGLQVRDSARELGRLTTVAVVQSIFLVILADALFAVLFVQIDF"
     gene            2401..>3209
                     /locus_tag="VGE92_15030"
     CDS             2401..>3209
                     /locus_tag="VGE92_15030"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY1315193.1"
                     /translation="MHNDPLLQILILLFASVCVVAAVRKLKLPAILGYLAVGMLLGPH
                     ALALAVDNDTTRLLADFGVVFLVFTLGLEFSLPRLVAMRWEVLGVGGAQVVVTSGAVA
                     AGAVMLFDINPAVAVVIGGAVAMSSTAIIISQLTEQSENNRTHGRLAVAICLFQDLSF
                     PLFLALVSVLSGAGQSVDAAHIVSAVGIAGLALLLVLAAGRWLLRPLFLLIAGVRSAE
                     LFSLAVLLAVLASAWATHAVGLSLALGAFLAGMMLAETEFRHQIEATIRSYR"
BASE COUNT          574 a         1026 c         1025 g          584 t
ORIGIN      
        1 ggtgcgtggt cagcgccggc aagctgctca acaatacgtc gaaatccgac ggcgcggagc
       61 gaatcacggg gtaatgcaaa ccatcggcaa gcacccccgg cgttgcggcc ttgtgatcct
      121 gctccagatc gatgaacagg aggcccgtga cgccctgcag gctcaaggat gcgcgggtgc
      181 tgccgtcgat cggcgcagtc gcttggatgt cggcgatcac cagcacgcga ttgcgctgtt
      241 gcggatcgat gagtattcga gtcaccttgc cgacgtcgac ccccaggtag cgcaccgggc
      301 tgccctgggt gaggccgctg acggtgccgg ggaaatagat ctcataggtc aagtaaatcg
      361 ccttgtcctg attgttcgtg taccagagca cgaacgagcc ggccatggcg atcaccagca
      421 gcacgaatgc gcccaccgcc acataattcg cgtccctatc catcggcgtc cttggcggca
      481 gcgcgcgccc gcgagccgtg gaaataatcg tgtatccaag gagtctggtt gtgcagcatt
      541 ccatccaggc tgtcggtggt catgcgcccg tcgacgatga cgccgactcg gctgcaggtc
      601 cgaaacaagg tatcgaggtc gtgggtaatc atgaccaccg tgagccgcag ctgggagtga
      661 aggtataaca gtagctcatc gaaggccgcg gcggaaatgg ggtccaagcc cgcggtcggt
      721 tcgtcgagca gcagcagctt gggttcgagg ctgagcgcac gtgccaacgc cgcgcgcttg
      781 atcatgccgc cggagagttg cgacggatac ttggccgcgg catccgccgc gagcccgacc
      841 agcgccaggt aaagctccgc cagctcatcc agggtctttt cggatgccgg atagtattcg
      901 cggatcggca gttggatgtt ctgtgcgacg gtgagcgaac tgatgagcgc gccgttctga
      961 aacgtcacgc cgtagcttct taccaaggcg ctgcggtcgg cggccgccaa ttgctgcaca
     1021 ttgcggccgt aaagctcgac gctccccgcg tccggctcct tcaaccccaa gatcgtgcgc
     1081 agcaggaccg actttccggc tcccgagccg ccgacgatgc cgaagatctc gttcgcccgg
     1141 acctccatgt cgagcccgtc gtgcaccacc tgcgcgccga aacggttgac caggccgcgg
     1201 acgcggatca cggggactga ggtcggctcc ggcatcagaa atcgatctgc acgaacagca
     1261 cggcgaacag cgcatcggcc agaatgacga gaaagatcga ttgcaccacg gcgaccgtgg
     1321 tcaatcgccc gagctcccgg gcggaatcgc gcacctgcag tccacgataa catccgacca
     1381 ttgcaatcac ggcggcaaac accggagctt tgatcaggcc cgcccaaaac gtcgtcggcg
     1441 ccagggattc gcgcagtcga ttggtgaact gttccaacgg tatgtgcaga ttgaggagcg
     1501 acagcaggcc gccgccggcc agacccatcg catccgcgat cacggtcaac agcggcagag
     1561 cgatgatcag cgccgtaagc ctcggcacca ccaacagttc gatgggattc atgcccatgg
     1621 cgcgcagcgc atccgtttcc tcgttgagct gcatgacgcc gatttcggct gcaaatgcgc
     1681 tgcccgagcg cccggcgacg atgatggcgg tcagcagcac ccccatttcg cgcagcaccg
     1741 aaatggtcac gagatcgacg acgaaaatgt cggcgccgaa gcggctcagc tgctgcgcgc
     1801 cgagatacgc cacgatgacg ctgatcagaa aggcgatcag cgacacgatt gggatcgcgg
     1861 tgatgccggt ttcgtaaata tgccgggtca ccgacgacaa gcgcaatgcc cgtggccggg
     1921 agaacgccgc gccgaggacc gtgacgatgc ggccgaaaaa ccccagcacg gcgcgcgctt
     1981 cgctgccctg tagcaccgcc catcgtccca gattcccgac ggacgtctcg atacccgggt
     2041 gcaggtacgg tgcggcctgc gccgcgtgtt gctgcgcctt gagtttctgc agcaattcca
     2101 aaaagtgcgg cggcgcgccg gcgtgctgca cgttcaagcg ctggctgccg atctcgccca
     2161 ggcgcatcag cagagcccac accggaccga tcccgggcag ttgcgcgtgc gaccaatcgc
     2221 agatcagcgt gccgtgcagc gtgccgggca ggctcttgag ctgcacctcg acctgtgcga
     2281 tcgacgccaa tgaccagtcg ccgttcaggt gtagatgcca atcctcgccg gctccttcga
     2341 gctcgaggac cgtcatactt tgactcatcg ctatatgcta gcaggcaatc ggaggccgcg
     2401 atgcataacg atccactgct gcaaatcctg atcctgctgt tcgcttccgt gtgcgtggtg
     2461 gcggcagttc gcaagctgaa attgcctgcg atactcggct atctcgcggt cggcatgttg
     2521 ctcgggccgc acgcactcgc gctggcggtc gacaacgaca cgacgcgtct tctggcggac
     2581 ttcggcgtgg tcttcctggt gttcaccttg ggactggaat tttcgctgcc gcggctggtc
     2641 gcgatgcgct gggaagtgct cggcgtcggc ggtgcgcagg ttgtggtcac gagcggcgcg
     2701 gtcgcagcgg gcgccgtcat gctgttcgac atcaaccccg ccgtggcggt ggtgatcggc
     2761 ggcgcggtcg ccatgtcatc gaccgcaatc atcatttcgc agctcaccga gcagtcggag
     2821 aacaaccgca cgcacggccg ccttgcggtg gcgatttgcc tgtttcagga tttgagcttt
     2881 ccgctgttcc tcgccttggt ttccgtgctg agcggcgccg gacaatcggt cgacgccgcg
     2941 cacatcgtga gcgccgtcgg catcgcgggg ctcgccctgc tgctggtgct cgccgccgga
     3001 cgctggttgc tgcggccgct gttcctgctg atcgcggggg tgcgctcggc cgagctgttt
     3061 tcactggccg tgctgctggc ggtgctggcc tcggcctggg cgactcacgc agtagggctg
     3121 tcgctcgcgc tcggcgcttt tctcgccggc atgatgctcg cggagaccga atttcgccac
     3181 cagatcgaag cgacgatccg ttcctatcg
//
LOCUS       DASWZT010000525         2124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_33484, whole genome shotgun sequence.
ACCESSION   DASWZT010000525 DASWZT010000000
VERSION     DASWZT010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33484"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VGE92_15035"
     CDS             <1..717
                     /locus_tag="VGE92_15035"
                     /inference="COORDINATES: protein motif:HMM:NF018168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HEY1315194.1"
                     /translation="KGPNGQIGKQASVTVTAGMTIGDIVGALNTAFGGAASFSLSSSG
                     ALTMTPSAANSGYQLNVVSDSTERGSTGMSFTQLFGLGTQQLTAQAQGFTVSSALTAN
                     PSSLPLGQPSITSSTAIGDPIVASGDSSGLDALDNVSNAQQTFQAAGTLSAQSTSLTN
                     YVGAFYQDIATQTQTATTNSTTEGDRLTEAQSLQSQTSGVNLDDELSKMVVYQQAYSA
                     GARLLQVAQALDQTLQQDLT"
     gene            729..1679
                     /locus_tag="VGE92_15040"
     CDS             729..1679
                     /locus_tag="VGE92_15040"
                     /inference="COORDINATES: protein motif:HMM:NF012903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEY1315195.1"
                     /translation="MTIGTVSTSAQSMLLISQLNQDNTALNQTQQQVSSGLVSTTYGG
                     YGDQTAALESAQAAVDRTNSYQAATQLALNQANLQDTQLTQLSSLASQLQQDITTAVA
                     NNTGSTLMASASSIYDQVTQILNSQDSNGNYIYGGDQNNTPPVTTTSLSDLASLSSVS
                     QAFTSGTVKGSVQVGDGQTVQVGLLASNIGTGILQTLSDIANFNTGSSGNFGSTLTGA
                     QSTFLSNEIPVSQSAGETVTNAAAQNGDTYQQLQGALTQQQAMNTLYTGFVSNIKNVN
                     MASAISSLNLDQTALQAATEVTAQLNQVSLLNYLSPTASG"
     gene            1724..>2124
                     /locus_tag="VGE92_15045"
     CDS             1724..>2124
                     /locus_tag="VGE92_15045"
                     /inference="COORDINATES: protein motif:HMM:NF015042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEY1315196.1"
                     /translation="MSTLNSLGIAKPASATRVVVAMSGGVDSSVTAALLKREGYDVLG
                     VTLQLYDQGVAVKKKGACCAGQDIHDAARVAAKIGIPHYVLDYEKRFRSEVIDDFADG
                     YVRGETPVPCIRCNETVKFRDLLDVARDLGA"
BASE COUNT          447 a          676 c          633 g          368 t
ORIGIN      
        1 aagggtccga acgggcagat cggcaagcag gcgagcgtca ccgtcaccgc cggaatgacc
       61 atcggcgaca tcgtcggcgc actcaacacc gccttcggag gggccgcgag cttctcgttg
      121 tcgtccagcg gtgcgctcac catgacgccg tccgcggcaa attcgggcta ccagttgaat
      181 gtcgtgagcg attccaccga gcgcggctcg acggggatga gtttcacgca attgttcgga
      241 ctcggcacgc agcaactgac cgcgcaggcg cagggcttca cggtcagctc cgctctgacg
      301 gcgaatcctt cgtcgctgcc tttgggccaa ccttcaatca ccagttcaac ggccatcggc
      361 gatccgatcg tggcgtcggg cgattcgagc ggcctcgacg ctctcgacaa tgtgtcgaac
      421 gcccagcaga cattccaggc tgcaggcaca ctgtcggcgc agtcgacatc gctgacgaac
      481 tatgtgggcg cgttctacca ggacatcgcg acccagactc agacggccac gaccaacagc
      541 accaccgaag gcgaccggtt gacggaagcg caatcgcttc agtcgcagac atcgggcgtc
      601 aatctcgacg acgagttgtc gaaaatggtg gtctatcagc aggcctatag cgccggcgcc
      661 cgcttgctgc aggttgccca ggcgctcgat cagacgttgc agcaagacct gacctgagga
      721 agcagcctat gacgatcggc accgtatcca ccagcgcaca aagcatgttg ctgatttcgc
      781 agttgaatca ggacaacacc gcgctgaatc agacgcagca gcaggtttcc tccgggctgg
      841 tttcgacaac ttatggcgga tatggcgatc agacggccgc gctcgaatcc gcacaagccg
      901 ccgtcgatcg cacgaattcc tatcaggcag cgacgcagct cgcgctcaac caggccaatt
      961 tacaggacac gcagctcacg cagctgtcct cgctcgcaag tcaattgcag caggacatca
     1021 caacggccgt ggcgaacaat accggttcga cactgatggc gagtgcatcg agcatctacg
     1081 atcaggtgac gcagatcctg aactcccagg attcgaacgg caactacatc tatggcggcg
     1141 accagaacaa cacgcctcca gtcacgacca cctcgctcag cgatcttgcg tcgctgtcat
     1201 cggtttcgca ggcattcacg agcggcacag ttaagggaag cgtgcaggtc ggtgacggcc
     1261 agacagtcca ggtgggtctt ctggcgtcca acatcggcac gggaatcctg cagacattgt
     1321 cggacatcgc caatttcaac accggctcca gcggaaattt cgggtcgacg cttacaggcg
     1381 cgcaatcgac gttcctgtcg aacgaaatcc ccgtttcgca atcggccggc gagaccgtga
     1441 ccaatgcggc ggcccagaac ggcgacacct atcaacagct gcaaggcgcg ctgacccagc
     1501 agcaagccat gaacacgctc tatacgggct tcgtctcgaa catcaagaat gtgaatatgg
     1561 cttcggcgat cagcagcctc aatctggacc agactgcgtt gcaggcggcc accgaagtga
     1621 cggcgcagct gaaccaggtt tcgctgctca actacctgtc gcccacggcc tccggctgat
     1681 ccggcttgcc acgggcaggc ggcggtccta tagggctgcc gccatgagca ccctcaactc
     1741 ccttggaatt gccaagcccg caagcgcgac ccgcgtggtc gtcgcgatgt cgggcggcgt
     1801 ggattcctcg gtcacggccg cgctactgaa gcgcgaaggc tatgacgtgc tgggcgtgac
     1861 gttgcagctc tacgatcagg gggtcgcggt caagaagaag ggcgcgtgtt gcgccggcca
     1921 ggacatccac gacgccgcgc gcgtggccgc gaagatcggc attccccatt acgtgctcga
     1981 ttacgaaaag cgtttccgct ccgaggtgat cgacgatttc gccgacggtt acgtgcgcgg
     2041 cgaaacgccg gtgccctgca tccgctgcaa cgagacggtg aagttccgcg acctgctgga
     2101 cgtggcgcgc gatctgggcg ctga
//
LOCUS       DASWZT010000526         2235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_67081, whole genome shotgun sequence.
ACCESSION   DASWZT010000526 DASWZT010000000
VERSION     DASWZT010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67081"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..2036)
                     /locus_tag="VGE92_15050"
     CDS             complement(189..2036)
                     /locus_tag="VGE92_15050"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HEY1315197.1"
                     /translation="MPARLACALLASMFIRRTRTSSRKTGDSYETFRLVEAVREGKTV
                     KQRTILNLGAHFDVLQADWAALAARIDEILHGQVGFLGVSEQIEAVAQRYAAQILARA
                     GREAEASPAEGAERFQEVDLASLELVRPRMVGVEHAALSAMDALGLPEKLAELGFTGP
                     QRAAALGNIIGRMARPASERATHDWLQHTTALGELVGYDYERLNLQQLYRSADQLLKH
                     REALEAHIFGAAQSLFGFENTITLYDLTNTYFEGTAAGNAKASRGHSKEKRGDCPLVT
                     LGLVLDVSGFPRRSRIFAGNVTEGTTLEAMLAELGATAGTNVVMDAGIATQANLAWLT
                     QAGYRYVVVSRKRERRFDPEQASLVQTASGMPVQVQRVDDLDTGETLLYCHSPARADK
                     DRAIDTTKAAGFETALQKLADGLAKPRGSKDPAKLHERIGRAKQKYPRAAQHYTIEVM
                     LDKPGQSVTAITWSKTPRPNSTVTHPGVYCLRTTLTEHDDATLGRIYSMLTNLESVFR
                     SLKTDLGLRPVYHQIERRVEGHLFISVLAYSVVHSMRLQLKAEAISDSWETIRNMLAT
                     QVRITTTLKRRDGRTIHVRKASRPEPRQQTIHAALKLTANPGGTQQTVV"
BASE COUNT          380 a          670 c          732 g          453 t
ORIGIN      
        1 agcagccgac gggcttgcgg cccgtctttc gatcgccgcg cctccaacct aacccgagtt
       61 cgacattttg cactacgcaa cggctgatgt ctgcggagtt tgggtgggtg acagcacgcg
      121 cttggcacta catttgtgca atctgcgctg acactgacag cacggcgcca cgcccctgac
      181 accccatctc acacgacggt ttgctgggtg cctccgggat tggcggtgag cttgagtgct
      241 gcgtggatgg tctgctggcg cggctcgggc cgtgaggcct tgcggacatg gatggtgcgg
      301 ccatcgcggc gcttgagggt ggtggtgatg cgaacctgtg tggcaagcat gttgcggatg
      361 gtttcccagg agtccgagat ggcctcggcc ttgagctgca ggcgcatgga atggacgaca
      421 ctgtaggcca ggacactgat gaacagatgg ccttcaacgc gacgctcgat ctggtgatac
      481 acgggccgca ggccaaggtc ggtcttgagc gaccggaaca cggattccag atttgtgagc
      541 atggagtaga tacgccccag ggtcgcatcg tcgtgctcgg tcagcgtggt gcgcaggcaa
      601 tagacgccgg gatgggtcac cgttgagttg ggcctggggg ttttgctcca ggtgatcgcg
      661 gtgacggact ggcccggttt atcgagcatc acctcgatcg tataatgctg ggcggcgcgg
      721 gggtatttct gtttggcccg gccgatgcgt tcgtggagct tggcggggtc tttggacccg
      781 cgtggtttgg caagaccatc ggcgagcttt tgcagcgccg tctcgaagcc tgcggccttt
      841 gtggtgtcga tcgcccggtc cttgtcggcg cgagctggcg aatgacagta gaggagcgtc
      901 tcgcctgtat cgaggtcatc gacccgctga acctgaaccg gcatgccgct ggcggtttgc
      961 acgaggctgg cctgctcggg atcgaagcga cgttcgcgct tgcgactgac cacgacatag
     1021 cgataaccgg cttgcgtgag ccacgccaga ttggcctggg tggcaatgcc ggcatccatc
     1081 acgacattgg tgccagcggt cgcacccagc tcagccagca tggcctcaag cgttgtgccc
     1141 tctgtgacat tgccggcaaa gatccggctg cgcctgggaa agccgctgac atcgagcacc
     1201 agtcccaggg taaccaacgg acagtcgccg cgtttttcct tggagtggcc gcgactggcc
     1261 ttggcgttcc cagccgcggt gccctcaaag taggtgttgg tcaggtcata gagcgtgatc
     1321 gtgttttcaa atccgaacag cgactgggcc gcgccgaaga tatgcgcctc gagcgcctca
     1381 cggtgtttga gcagctggtc tgcgctgcga tagagctgct ggaggttgag ccgctcgtaa
     1441 tcgtatccga cgagctcacc gagcgcggtg gtgtgctgca gccagtcatg ggttgcgcgc
     1501 tcgctggcag ggcgcgccat gcggccgatg atgttgccaa gcgcggccgc acgctgcggg
     1561 ccggtgaagc cgagctccgc aagcttttcg ggcaggccga gcgcatccat cgccgagagc
     1621 gcggcatgct cgaccccgac catacgcggc ctgaccagct cgagcgaggc cagatcaacc
     1681 tcctggaagc gctcggcgcc ctcggccggg ctggcttcgg cctcccgtcc cgcacgcgcg
     1741 aggatctggg ctgcgtagcg ctgggccacg gcctcgatct gttcggacac gcccagaaac
     1801 ccgacctggc cgtgcaggat ctcgtcgatc cgcgctgcga gcgccgccca gtcggcttgc
     1861 agcacgtcga aatgcgcgcc caggttgagg attgtgcgct gtttgacagt ctttccctcg
     1921 cgcacggcct cgaccaggcg gaaggtttca tagctatcgc cagtcttgcg gctgctggtc
     1981 ctggtgcggc gaatgaacat cgacgcaagc agggcacagg ccagccgcgc aggcaatgcc
     2041 cattcctcaa attatggcac tacactttta ctttcgccat caaacccacg gttttccgcc
     2101 attttcctcg ccgaaatccc gcagaatcga cgaaatctcg gcgaaatcgc gggcaaaatg
     2161 tcaaagatgg gctaaccggt tgcttctatg tcatcgttct ctcccgcttt gtcgcagtgg
     2221 atgatagctg ctaag
//
LOCUS       DASWZT010000527         3582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_18650, whole genome shotgun sequence.
ACCESSION   DASWZT010000527 DASWZT010000000
VERSION     DASWZT010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18650"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VGE92_15055"
     CDS             <1..678
                     /locus_tag="VGE92_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315198.1"
                     /translation="QFLRQRQAGVQALRLKLKHRAAPATCVQLGLASITSERCRLMDV
                     LTQKLLTLELAAPVRGVELISGSLQPLPASSLDVFAGLSRAGTGGGRASAPQLVERLR
                     ARLGEHAVYGVISIPEHRPEAAWRRVHELSLTAAGRGAMTDPKITAHAMPRPVWLLDA
                     PQPLADGLVLEEGPERIESGWWDGKGVARDYYVAREACAGHAYGAKLWVFQERQSKRW
                     YLHGMFA"
     gene            720..>3582
                     /locus_tag="VGE92_15060"
     CDS             720..>3582
                     /locus_tag="VGE92_15060"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEY1315199.1"
                     /translation="MSNFSFLRGASQPEELIAQAKRLDYRGLALTDECSLAGVVRAHV
                     AAKQHGLPLIIGTEFICADALKFVALATDRASYGALSRLISRARRANIKGRYTLARTD
                     LENALDGCLLIWLPCADRALAQRQEHDGRWLRERFPGRVWVGVELLTSGCDTRRLEQL
                     ETLGQTLGLPRVAAGDVHMHRRGRRALQDALTAIRLNTPLQALGFAVFPNGERCLRPL
                     QRLRELYPAPLLAQTLEIAERCTFNLDELRYEYPEEIVPAGTTPTGHLRELTLRGCAQ
                     RWPAGVPAAVRENIEHELRLIAELKFEPFFLTVHDVVHYARSQGILCQGRGSAANSTV
                     CYCLHITEVDPSRMAMLFERFISKERNEPPDIDVDFEHERREEVIQYIYEKYGRERAA
                     LAATVICYRPRSALRDVGKALGLDLAQVDRLARGMQWWDGQRIDPDRIRASGFAPDDP
                     LIARLIALAAEILGFPRHLSQHVGGFVIARGRLDELVPIENAAMPDRTVIEWDKDDLD
                     ALGLLKVDVLGLGMLSALRRAFHLVNEFRGATPGQGELDLATVPTEDKAVYEMICRAD
                     TTGVFQIESRAQMSMLPRLRPQNYYDLVIEVAIVRPGPIQGEMVHPYLRRRSGEEAVT
                     YPSKAVEAVLKRTLGVPIFQEQVMQLAIVAAGFSPGEADRLRRAMAAWKRKGGLEPFQ
                     RQLIDGMRERGYEESFARQIFNQILGFGEYGFPECVVGETRVVDADTGKWLTIDEIIN
                     GARFDHTLACDEDLRLRKRKVLRVIRSGAKPVWRLRTALGHEIIATAEHPFMTMAGWI
                     QLGNLKIGGLVATARCAPVQGRRSRSDVYWDRIVEIEAVGTRETYDLQIEGDRNFLAN
                     HFVVHNSHAASFALLVYTSAWLKHYEPAAFCAALINSQPMGFYAPAQLVRDARSHGVE
                     VRVVDAALSEWDCTLERRADGRPALRLGLRLVKHLSSDGA"
BASE COUNT          646 a         1054 c         1181 g          701 t
ORIGIN      
        1 cagtttttgc gccagcgcca agccggcgtc caggcattgc ggctgaagct caagcatcgt
       61 gcggcacctg cgacttgcgt acagctggga ctggcgagca tcacgagtga gcgctgccgt
      121 ttgatggatg tattgaccca gaaattattg acgctcgagt tggcagcacc ggttcgaggc
      181 gtggaactga tttccggttc tctgcagcca ctgcccgcca gctcgctcga tgtctttgcg
      241 ggattgtcgc gcgccggcac aggcggcgga cgcgccagcg cgccgcaatt ggtggagcgc
      301 ttgcgcgcac gtttgggcga gcatgcggtg tatggggtaa tttcaatccc ggaacatcgg
      361 cccgaagctg cgtggcggcg agtccacgaa ttgtcgctga ccgccgccgg acgcggcgcc
      421 atgaccgatc ccaaaatcac cgctcatgcg atgccgcgcc cggtttggtt gctggatgca
      481 ccgcagccgc tggctgacgg tttggttctg gaggagggtc cggaacgcat cgagagcggt
      541 tggtgggacg gcaaaggcgt ggcgcgcgat tactacgttg cccgcgaagc ctgcgctggc
      601 cacgcgtacg gcgcaaagct atgggttttt caggaacgtc aatccaagcg ttggtatcta
      661 catgggatgt tcgcttaaac ggcgtcatcg cttgagacgt gtacgccgaa ctccacgctg
      721 tgagcaattt ttctttcttg cggggcgctt cgcagcccga agaattaatc gcccaggcta
      781 aacgtctcga ttatcgcggg ttggcgctca ccgacgaatg ttcactcgcg ggtgtggtgc
      841 gcgctcacgt cgccgccaag cagcacggtc tgccgctgat catcggcacg gagttcatct
      901 gcgccgatgc tctgaagttc gtggcgctgg ccaccgatcg ggcaagttat ggcgctctca
      961 gccgcctgat cagccgggca cggcgcgcaa atatcaaggg ccgttacacc ctggcacgca
     1021 ccgacctcga gaatgccctc gatggatgct tgctcatctg gctgccgtgc gccgaccgag
     1081 cgttggcaca gcgccaggag cacgacggcc ggtggttgcg tgaacgcttc ccggggcgtg
     1141 tctgggtggg cgtggaatta ttgacttcgg ggtgcgacac gcgccgtctc gaacagctgg
     1201 aaacattggg ccaaacgctg ggattacccc gcgtggccgc gggcgatgtg cacatgcatc
     1261 ggcgcggccg ccgcgccctg caagatgcct tgacggcgat ccgcctgaac acgccgctgc
     1321 aagccttggg gtttgccgta tttccgaacg gcgagcgctg tttacggccc ttgcagcggc
     1381 tgcgcgagtt atatcccgcg ccgctgctcg cgcaaaccct ggagattgcc gagcgctgca
     1441 cgttcaatct cgatgaattg cgctacgagt atcccgagga aatcgtccct gccggcacca
     1501 ctcccaccgg tcatttgcgc gaactgacgc tgcgcggctg tgcgcagcgt tggccggcgg
     1561 gcgtgccggc ggcggtgcgc gaaaacatcg aacatgaatt gcgcttgatc gccgaactca
     1621 agttcgagcc gttctttctg accgtgcacg atgtcgtgca ctatgcgcgc tcccaaggaa
     1681 tactctgtca aggccgcggc tccgcggcca actccacggt ctgctactgc ctgcacatca
     1741 ccgaggtgga cccctcgcgc atggccatgc tgttcgaacg cttcatctcc aaagagcgca
     1801 acgaaccgcc ggatatcgac gtggatttcg agcacgagcg gcgcgaagag gtgattcagt
     1861 acatctatga aaaatacggc cgcgagcgtg cggcgctggc ggccacggtg atttgctacc
     1921 ggccgcgcag cgcactgcgc gacgtcggca aggcgctcgg cttggatctt gcgcaggtcg
     1981 acaggctcgc gcgcggcatg caatggtggg atggacagcg catcgatccg gatcgcatcc
     2041 gcgcctcggg atttgctccg gacgacccgc tgatcgcgcg cctcattgcg ctcgcggcgg
     2101 agatcttggg gtttcctcgg catctttcgc agcatgtcgg cggcttcgtc atcgcacgcg
     2161 gacgcttgga tgaactggtc cccatcgaga acgcggccat gcccgatcgc acggtgatcg
     2221 agtgggacaa agacgatctc gatgcgctcg ggcttttgaa agtcgatgtg ctggggctcg
     2281 gcatgttgag cgcgctgcgc cgtgcatttc atctcgtcaa cgaattccgc ggcgcaaccc
     2341 ccggccaggg cgaactcgat ctggcgacgg tgccgaccga ggataaagcc gtgtacgaga
     2401 tgatctgccg cgccgacacc acgggtgtgt ttcaaatcga atcgcgggcg cagatgtcca
     2461 tgttgccgcg cctgcggccg cagaattact acgatctggt gatcgaagtg gcgatcgtgc
     2521 gacccggccc gatccaaggc gaaatggtgc acccgtactt gcgccggcgc agcggcgagg
     2581 aggcggtgac ctatcccagc aaggcggtcg aggcagtgct caagcgcacg ttgggagtgc
     2641 cgatttttca ggagcaggtc atgcagctcg cgatcgtcgc ggccggcttt tcgccgggcg
     2701 aagcagaccg gctgcgccgc gcgatggcag cctggaagcg caagggcgga ctggagccgt
     2761 tccaacggca attgatcgac ggcatgcgcg agcgcggcta tgaggagagc ttcgctcggc
     2821 agatattcaa tcagattctc ggttttggcg aatatggatt tccggaatgc gtcgtcggcg
     2881 agacgcgcgt agtggatgcc gataccggaa aatggctgac catcgatgaa atcatcaatg
     2941 gggcgcgctt cgatcacacg ctcgcgtgcg atgaggatct gcgtttgagg aagcgcaaag
     3001 tgctgcgcgt tatacgcagc ggcgcgaagc cggtatggcg cctgcgaacg gcgctcggcc
     3061 acgagattat cgcgacggct gaacacccat tcatgaccat ggctgggtgg attcagctgg
     3121 gaaatctcaa aattggcggc cttgtggcaa ctgcgcgctg cgcaccggta caggggcggc
     3181 gatcacggtc cgatgtctat tgggacagaa tcgtggagat cgaggcagtc gggactcggg
     3241 aaacatacga tttgcagatc gaaggcgatc gcaatttctt ggccaatcat ttcgttgtcc
     3301 acaattcgca cgcggcaagc ttcgcactcc tcgtctacac ctccgcctgg ctgaagcatt
     3361 acgagcctgc ggcgttttgc gcggcgctca tcaacagcca accgatgggt ttttacgcgc
     3421 cggcgcaatt ggtgcgtgat gcacgctcgc atggcgtgga ggtacgcgtc gtcgacgcgg
     3481 cattgagcga gtgggattgt acgctggagc ggcgggcgga tggccgcccg gcgctgcggt
     3541 tgggattgcg tttggtgaag catttatcga gcgacggagc gg
//
LOCUS       DASWZT010000528         4437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_81822, whole genome shotgun sequence.
ACCESSION   DASWZT010000528 DASWZT010000000
VERSION     DASWZT010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81822"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VGE92_15065"
     CDS             <1..627
                     /locus_tag="VGE92_15065"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY1315200.1"
                     /translation="TVQRIVAALVEEQLLMSASLKARVKLGPGLVQLAAAADVGTEKL
                     VRPFMVELSRVADETVDLSVLKRDAAVFVEQIQGTQRLAAVSAVGKEFPLHCTANGKA
                     LLSLMTPERRERLLAGRLKRFTEATVTDRAALEEVLREGEKTGLAYDLEEHSVGICAV
                     GTAFFDSIGREYSLSIPVPATRFEAKKQLLSKLLKKAKADLLEHIVAG"
     gene            671..1321
                     /locus_tag="VGE92_15070"
     CDS             671..1321
                     /locus_tag="VGE92_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315201.1"
                     /translation="MKCSVSHDSLGTHLDWQHENVTARMIDWFWSNMEKGFILWHPEQ
                     HEALEWAVPPKHGNPLGAIHIAPQTWNDGRRQNLYIRFERLDDVPPHIRDIIEYEHVI
                     VVAGLGLGPECLSKPDPMGYRIHQWQKTDAGVVGKSSAIGTRKMETVEDGKVWAAHCA
                     EEVGNWGVFLPQLYTLYQVVTDTGRNPYADLSVAGVGRAARYRCIGAGDQATAAGR"
     gene            complement(1297..1935)
                     /locus_tag="VGE92_15075"
     CDS             complement(1297..1935)
                     /locus_tag="VGE92_15075"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY1315202.1"
                     /translation="MNSKAAAAPAPPRVDKAAQILRAARAEFLLHGYAATSMDAVALT
                     AKVSKATLYVYFVSKRELFAAIIQQERSRFNVSMLSGESGREPIQSKLLRFGRNIVDF
                     LISPETIASYRMVAAEAGRLPELGEEFYSNGPVKFLDRLEEFMDRAMHGGTLRRDDPR
                     RAAEQLIGLVRGDLQLRALLGVTNGLTRARIDAVIRAGVDTFYRAYRPAAVA"
     gene            2057..3547
                     /locus_tag="VGE92_15080"
     CDS             2057..3547
                     /locus_tag="VGE92_15080"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HEY1315203.1"
                     /translation="MHSTLEAATEVSAVKFPDLLIYRDYAAPVRIEGDVYDLEVQGRI
                     PCELNGAYYRMSADPQYPPLLGKDIFINGDGMMHMLRFDDGHADLKMRYVRTPKWAAE
                     RAARRALFGAYRNPFTDDPSVKGIDGNTANTTAFWHHGRLYALKEAARPMRMDPLTLE
                     TLGSWDFHGKLLSKTFTAHPKSDPVTGEVIAFAYNTAGRSSREIEIYWISAQGRITRT
                     ESFMAPYSFMAHDCLVSRNYIALTVYPMVSEWERIERGEPYFHWDSSLPTRVVIIPRR
                     EGVAALRWYTCPFTGMETHSFNAWEEGELLHLDHFFTASGWLSQFPDIRGVAFKEEPP
                     HAERWTFDLANRSNEFSVRRIFEQIGEMPMIDPRFLMSRARHFYFGTNNPALGPMLEW
                     GPKGPPFTCIGHFDEARGKMNFWYSGPDSSPEEPFFVPRSPDASEGEGWLLSVVGRRR
                     ENRTDLVILDAAHLERGPVATIKMPCRLHEGFHGTWVSAAELAAHT"
     gene            3588..>4437
                     /locus_tag="VGE92_15085"
     CDS             3588..>4437
                     /locus_tag="VGE92_15085"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY1315204.1"
                     /translation="MSLNETHDPTLRSWLASANAQGADFPLQNLPFAVFRRSGSGEAF
                     RGGVAIGDQVLDLAAARASSGFHSSLGRLAAGALDSAARSSLNELMSLGAESWSALRL
                     ALSRALRAASERQAGLQACLCPQDKAEFALPAQIGDYTDFYTSIHHATAVGKLMRPDN
                     PLLPNYKWVPIGYHGRVSSIGVSGQAFPRPAGQKMAAGAATPSVGPSERLDYELELGI
                     FIGQGNESGARISIARAESHIFGLCLLNDWSARDLQGWEYQPLGPFLGKNFATTISPW
                     IVTLEAL"
BASE COUNT          843 a         1324 c         1388 g          882 t
ORIGIN      
        1 accgtgcagc gcatcgtggc ggccttggtc gaggagcagt tgctgatgtc ggcctcgttg
       61 aaggcgcgcg tcaagctggg cccgggattg gttcagctcg ctgccgccgc cgatgtcggc
      121 acggaaaagt tggtccgtcc cttcatggtg gaattgtccc gcgtggccga cgagaccgtg
      181 gatctgtcgg tgttgaaacg cgacgcggcg gtttttgtcg agcaaatcca aggcacccaa
      241 cgcctggccg ccgtctcggc ggtgggcaag gaatttccgt tgcattgcac tgcgaacggc
      301 aaggcgcttt tgtcattgat gacgcccgag cgccgcgagc gcctactcgc gggcaggctc
      361 aagcgcttca ccgaggcgac ggtgacggat cgcgcagcgc tcgaggaagt gctgcgcgag
      421 ggcgaaaaaa ccggtcttgc ctacgatctc gaagagcaca gcgtcggcat ctgcgctgtc
      481 ggcacggcgt ttttcgattc catcggccgt gaatattcgc tgtcgatacc ggttccggcg
      541 acgcgcttcg aagcgaaaaa gcagttgctg tcgaaactcc tcaagaaagc caaggccgac
      601 ttgctcgaac acatcgtcgc tggctgagcg acgctctcgt cattcacttg aggactgaaa
      661 tacaatggcc atgaagtgca gtgtgtcgca cgactcgctc ggcacgcatc tggactggca
      721 gcatgaaaac gtgaccgcgc gcatgatcga ttggttctgg agcaacatgg agaagggctt
      781 cattctctgg catccggaac aacacgaagc gctcgaatgg gcggttccgc ccaagcacgg
      841 caacccgctg ggcgcgatcc acatcgcgcc gcagacctgg aatgacggac gccgtcaaaa
      901 cctctacatc cgtttcgaac gccttgatga tgtgccgcct cacatccgcg acataatcga
      961 atacgagcat gtaatcgtgg tcgcaggctt gggcttgggc cccgagtgcc tgagcaaacc
     1021 ggatccgatg ggctatcgta tccatcagtg gcagaaaacc gacgccggag tcgtcggcaa
     1081 gtcttccgcc atcggcacgc gcaagatgga aacggtcgag gacgggaaag tgtgggcggc
     1141 gcattgcgcc gaggaagtgg gcaactgggg tgtatttctg ccgcagctgt acacactgta
     1201 tcaagtggtc accgacaccg gccgtaatcc ctacgccgat ctgagcgtcg caggcgtcgg
     1261 ccgagcggcg cgttaccgct gcattggcgc gggcgatcag gccaccgccg ccggccggta
     1321 ggcccggtaa aaggtatcga cgcccgcgcg aatgacggca tcgatccgcg cccgagtcag
     1381 tccattcgtc acccccaaga gtgcacgcag ctgcaaatcg ccgcgcacca agccgatcaa
     1441 ttgttccgcg gcgcggcgcg gatcgtcgcg gcgcaacgtg ccgccatgca tcgccctatc
     1501 catgaactcc tcgaggcgat ccagaaactt gaccggccca ttcgagtaaa actcttcgcc
     1561 caattccggc aacctgccgg cttccgccgc gaccatgcga taggacgcga tcgtctcggg
     1621 ggatatcaag aaatcgacta tgttgcgccc gaagcgcagc aacttggatt gaatcggctc
     1681 gcggccgctc tcgccgctca gcatcgagac gttaaagcgg ctgcgctctt gctgaatgat
     1741 ggctgcgaac agctcccgct tgctcacgaa atagacgtac agagtcgctt tactgacttt
     1801 ggcggtgagc gcgaccgcgt ccatgctggt ggcggcataa ccgtggagca ggaattctgc
     1861 gcgcgcggcg cgcagaattt gcgcggcctt atcgacgcgg ggcggcgctg gtgcggcggc
     1921 tgctttggaa ttcacgggtt tctcgttgcg tcgttaaaac cagagagttt agcaattgac
     1981 catgtttggt gctcagtgta gggtctgtgc atgatagtac taaaccatat agtttagcca
     2041 tcgcgaggtc accgccgtgc attccaccct cgaagccgct accgaagtga gcgctgtcaa
     2101 atttccggat ctgctgatct accgcgacta cgcggcgccc gtgcgcatcg agggcgacgt
     2161 ctatgacctg gaagtgcaag gtcgaatccc atgcgagctc aacggcgcct actaccgcat
     2221 gagtgccgat ccgcagtatc cgccgctgct cggcaaggac attttcatca acggcgacgg
     2281 catgatgcat atgctgcgct tcgatgacgg tcacgcggac cttaagatgc gctacgtgcg
     2341 cacgcctaaa tgggctgcgg agcgcgccgc gcgccgcgcg ttgtttggtg cttaccgcaa
     2401 tccgttcacc gacgatccga gcgtcaaggg gatcgacggc aatacggcca acaccacagc
     2461 gttttggcat catggaaggc tatacgcgct caaagaagcc gcgcggccga tgaggatgga
     2521 cccactcacg ctcgagaccc tgggcagctg ggatttccat ggaaagctgt tgagcaagac
     2581 gttcacggcg catccgaaga gcgatcccgt gaccggcgaa gtcatcgctt tcgcatacaa
     2641 cacggccggg cggagcagcc gggaaatcga gatctattgg atatccgcgc agggaagaat
     2701 tacccgcacg gaatcattca tggcgcccta ttcgttcatg gcgcacgact gcttggtttc
     2761 gaggaactat atcgcgctga cggtatatcc gatggtgagc gaatgggagc gaatcgagcg
     2821 cggcgagccc tactttcatt gggactcgag tcttccgacg cgggtcgtga tcattccgcg
     2881 gcgtgaagga gtcgctgctc ttcgctggta cacctgccct ttcacgggta tggaaactca
     2941 ctcgttcaac gcctgggagg agggcgagtt gctgcacttg gatcattttt ttaccgcctc
     3001 cggatggctg tcgcaatttc cggatattcg cggtgtcgct ttcaaagagg agccgccgca
     3061 tgcggagcgc tggacctttg acttggcgaa cagatccaat gagttctccg tgcggcggat
     3121 cttcgagcaa atcggcgaga tgccgatgat cgatccgcgc tttctcatga gccgcgcgcg
     3181 gcatttctat tttggaacca acaaccccgc gctggggccg atgttggaat ggggcccgaa
     3241 gggtccgccg tttacttgca tcggccactt cgacgaggcg cgcggcaaga tgaacttctg
     3301 gtattcaggt ccggactcct cgcccgaaga gccgttcttc gtgccgcgat cgcccgatgc
     3361 gtcggagggc gagggatggt tgttgtcggt tgtggggcgg cgccgcgaaa accgcacgga
     3421 ccttgtgatc ctggatgctg cccatctcga gcgcgggccg gttgcaacca tcaagatgcc
     3481 gtgccgcctg cacgaaggat tccacggcac gtgggtatcg gccgcggaac tcgccgcgca
     3541 tacctgatcc tggcacaatc cgccggctta agtgacaatc ccgctccatg tccctgaacg
     3601 agacacacga tccgacgctt cgcagctggc ttgcttcggc gaatgcgcag ggtgctgatt
     3661 tccctctgca gaacctgccg ttcgcggttt ttcgccgcag cggcagcggc gaggcctttc
     3721 gcggcggggt cgccatcggt gaccaagtgc tcgacctggc ggcggcgcgg gcgagcagcg
     3781 gatttcactc gagcttaggg cgcctcgctg ccggcgcgct cgacagcgcc gcgcgttctt
     3841 ccttgaacga actgatgagt ttgggtgccg aatcctggtc ggcgttgcgt ttggctctgt
     3901 cgcgcgcgct gcgcgctgca tcggagcgcc aagccggatt gcaagcgtgc ctttgccccc
     3961 aggataaagc tgaattcgcg ctgccggcgc agatcggcga ttacacggac ttctatacct
     4021 cgattcacca tgcgaccgcg gtgggcaagc tcatgcggcc ggataatccg ctgttgccga
     4081 attacaagtg ggtgccgatt ggctaccacg gccgcgtctc gtccatcggc gtatccgggc
     4141 aggcgtttcc gcggcccgct ggacagaaaa tggcggccgg ggctgcgacc cccagcgtcg
     4201 gacccagcga gcgtctggac tacgaactcg agcttgggat cttcatcggt cagggcaacg
     4261 agtccggcgc gcgcatttcc atcgcgcgcg ccgagtcgca tatctttggc ctgtgcctgc
     4321 tcaatgattg gtcggcccga gatcttcaag gctgggaata tcagccgttg ggcccgtttt
     4381 tggggaagaa ttttgcgacg acgatatcgc cgtggatcgt gacgctcgag gcgttgg
//
LOCUS       DASWZT010000529         1756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_52680, whole genome shotgun sequence.
ACCESSION   DASWZT010000529 DASWZT010000000
VERSION     DASWZT010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52680"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VGE92_15090"
     CDS             complement(<1..434)
                     /locus_tag="VGE92_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1315205.1"
                     /translation="MNVPASRARTEWIGFKTIIIREFSRIVRIWGQTIVPPAVTATLY
                     FIIFGSLIGRRVGQVGGFDYMQFIAPGLIMMTVITNSYGNVVSSFFGAKFGKHLEELL
                     VSPLPNWLIVAGYMGGGILRGVLVGSVVTIVSLFFTRLAVTHV"
     gene            complement(431..1378)
                     /locus_tag="VGE92_15095"
     CDS             complement(431..1378)
                     /locus_tag="VGE92_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004815237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1315206.1"
                     /translation="MQALVLRQLTKTYKNGIKALKAIDLTVEEGDFFALLGPNGAGKT
                     TAIGIVTSLVNKTSGTVEVFGHDIDRELEVAKSCIGLVPQEINFNQFETCFTIVVNQA
                     GFYGIPRPLAKQRAEKYLKQLQLWERRNSIARSLSGGMKRRLMIARALMHEPRLLILD
                     EPTAGVDIEIRRSMWEFLRNINDAGTTIILTTHYLEEAENLCRNVAIIEGGNIIERDS
                     MANVLRKLQTEVFVFNLREARSTPPTLPGFAVTLADEHTLEVEMSKSQTLNEIFAQLS
                     AQGIAVNSMRNKVNRLEELFIRLVDTSVSAGRAKIAASP"
     gene            complement(1442..>1756)
                     /locus_tag="VGE92_15100"
     CDS             complement(1442..>1756)
                     /locus_tag="VGE92_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315207.1"
                     /translation="DDATVCFVRLALFFAWHVASSAGVAAQLLLGMNLNTAAAFRRTP
                     LNRLPALVASETAHLSARWSGCKAYWRALVAAASRTDPAALRRVQLYGLQLAAASRLE
                     EP"
BASE COUNT          344 a          523 c          549 g          340 t
ORIGIN      
        1 acatgcgtca cggcgagccg cgtaaagaac agcgaaacga tggtcaccac ggatcccacc
       61 agcacgccgc gcagtatgcc tccgcccatg tagcccgcaa cgatcagcca gttgggcagc
      121 ggtgagacca gcagttcctc caagtgcttg ccgaatttcg cgccgaagaa cgaggacacg
      181 acattgccgt aggaattggt gatgacggtc atcatgatga gaccgggtgc gatgaactgc
      241 atgtagtcga agccgccgac ttggccgacg cggcggccga tcaggctgcc gaatatgatg
      301 aagtacagcg tcgcggtcac cgccggcggc acgatcgtct gtccccagat gcgcactatg
      361 cggctgaact cgcgaatgat gatggtcttg aaaccgatcc actcggttcg ggcgcgcgac
      421 gcaggtacgt tcatggcgag gcggcgattt ttgcgcggcc ggcggagacg ctggtatcga
      481 ccaggcgaat aaatagttct tcgagccggt tcaccttgtt gcgcatgctg ttgaccgcga
      541 ttccttgcgc cgacagctgt gcgaatatct cattgagagt ctgcgatttc gacatctcca
      601 cttccagcgt gtgctcatcc gcgagcgtga cggcgaaccc gggcaaggtg ggcggcgtgc
      661 tgcgcgcctc gcgcagattg aagacgaata cttcggtctg cagtttgcgc aacacgttgg
      721 ccatgctatc gcgctcgatg atgttgccgc cctcgatgat cgccacgttg cggcaaagat
      781 tttccgcctc ttcgagatag tgcgtcgtga ggatgatggt cgttcccgca tcgttgatgt
      841 tgcgcagaaa ttcccacatg gagcggcgga tttcaatgtc gacgcctgcg gtcggttcgt
      901 ccaggatcag caatctcggc tcgtgcatga gggcgcgcgc aatcatcagg cgccgcttca
      961 tgccgccgga cagggaacgc gcgatcgaat tgcgccgctc ccacaattgc agctgcttga
     1021 gatacttctc ggcgcgttgc ttcgcgagcg gccgcggtat cccgtagaac cccgcttgat
     1081 tgacgacaat ggtgaaacag gtctcgaact gattgaaatt gatttcctgc ggcaccaggc
     1141 cgatgcagga cttggcgact tcgagctctc gatcgatgtc gtgaccgaac acctcgaccg
     1201 tgccgctggt cttgttgacc agcgatgtga cgatgccaat cgcggtggtc ttgccggcgc
     1261 cgttcggacc gagcagggcg aagaaatcgc cctcttcaac ggtgaggtcg atggccttga
     1321 gcgccttgat gccgttcttg taggtcttgg tgagctgacg aaggactaag gcttgcatag
     1381 gaaccgctta ggttaaacct cgcgaggccc ttgccgcaac gccaatcgcg ttgctgcggc
     1441 gtcaaggttc ctccaggcgc gacgcggccg caagctgcag cccataaagc tgtacgcgcc
     1501 gcaacgccgc cgggtcggta cgcgacgccg cggcaacgag ggcgcgccag taggccttgc
     1561 atccgctcca gcgtgccgac aagtgggcgg tctcgctcgc caccaatgcg ggcaggcggt
     1621 tcaagggtgt acggcggaat gcggcggcgg tgttcagatt catgcccaac agcaactgag
     1681 ccgccacgcc cgcgctggag gctacatgcc acgcgaaaaa cagcgccaga cgcacgaagc
     1741 agaccgtggc atcgtc
//
LOCUS       DASWZT010000530         1566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_62306, whole genome shotgun sequence.
ACCESSION   DASWZT010000530 DASWZT010000000
VERSION     DASWZT010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62306"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..634)
                     /locus_tag="VGE92_15105"
     CDS             complement(188..634)
                     /locus_tag="VGE92_15105"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1315208.1"
                     /translation="MTYHIAPLRQSERERWTQLWSEYQVFYGVELPTAVTESLWQRLH
                     GGRVKGLGARDSADKLRGIVHFLFHEDTWSTAPACYLQDLYVDPTTRGTGCGRQLIEA
                     VARAAKTVGANPPYWLTHETNSVARQLYDRLGKNQGFIQYQYAPTG"
     gene            complement(631..>1566)
                     /locus_tag="VGE92_15110"
     CDS             complement(631..>1566)
                     /locus_tag="VGE92_15110"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEY1315209.1"
                     /translation="VRIKVQACGICHSDSITKLGVFPGIQYPRVPGHEVVGVIDAIGA
                     EVPDWKLGATVGVGWHGGHCGHCHSCRRGDFITCSTGQIPGISYDGGYADFMIAPFEA
                     LASVPQELSFADAAPLLCAGITTFNALRHSGAQSGDLVAILGIGGLGHLGVQFAAKMG
                     YRTVAIARGADKEPLARELGAHHYIDSTARSVAKALTKLGGARVILATATDSKSMSAA
                     IGGLGIDGKLIIVGAPSEPLEISPLTMIMGRRCIQGWPSGTAPDSEDTLDFSVLSQVR
                     PKIETFPLERAPEAYERMMSGKARFRVVLTMDGVK"
BASE COUNT          297 a          511 c          470 g          288 t
ORIGIN      
        1 ctggtcgaaa atgaaatcga acaccttgcc cttcggcact cctgcctgcg gcagcgagtc
       61 cggaccgcgc tggtattggt catcgggtga gggttgcgac tgtgcgacgc cgaggacggg
      121 tgcgcacatt gccgcggccg ccaccaatac gcgaatccgc agggcttttt tttaaactgc
      181 tcgcccacta accggtcggt gcgtactgat actgaataaa tccctggttc ttcccgagac
      241 ggtcgtacag ctgccgcgcg acgctgtttg tttcatgagt caaccagtaa ggcggattgg
      301 cgcccaccgt cttggctgcg cgcgcgacag cttcgatcaa ctggcggccg catcccgtgc
      361 cgcgtgtcgt gggatcgacg taaaggtctt gcaagtaaca cgcgggtgcg gtcgaccagg
      421 tgtcctcgtg aaacaggaaa tggacgatgc cgcgcagctt atcggccgaa tcacgggcac
      481 cgagaccttt gacccggccg ccgtgcaggc gctgccagag actctcggta accgcggttg
      541 gcagctcgac gccgtagaaa acctggtatt cgctccacag ctgggtccat cgctcccgct
      601 ccgactgtcg cagcggcgct atgtggtatg tcatttgacg ccgtccatgg tgagcaccac
      661 ccgaaaccgc gccttgccgc tcatcatgcg ctcgtaggct tcgggcgcac gctcgagcgg
      721 aaaggtttct atcttcggac gaacctgcga gaggacgctg aaatccagcg tgtcctccga
      781 atccggcgca gtgcccgacg gccagccttg aatgcagcgt cgacccatga tcatggtgag
      841 cggggaaatt tccagcggct cgctcggtgc gccgacgatg atcagcttgc cgtcaatccc
      901 caatccgccg atcgcggcgc tcatcgattt cgaatcggtc gccgtcgcca ggatcactcg
      961 cgcgccgccg agtttggtca gcgccttggc aacgcttcgc gccgtgctgt cgatgtagtg
     1021 atgcgcaccc agctcgcgag ccaagggttc cttatcggcg ccgcgggcaa tggcgaccgt
     1081 gcgataaccc atcttggcgg caaactgcac tcccaagtgc cccaaaccgc cgatgcccag
     1141 tatcgcgacc agatcacccg actgcgcccc gctgtggcgc aacgcattga atgtggtgat
     1201 gccggcgcac agcagcggcg ccgcatccgc gaacgacaat tcctgcggaa ccgaggcgag
     1261 cgcctcgaac ggtgcgatca tgaaatcggc gtatccgcca tcgtaagaga tacccggaat
     1321 ttggccggtc gagcaggtga tgaaatcacc gcgacggcag gagtggcagt gcccgcagtg
     1381 gccgccgtgc cagcccacac cgaccgttgc acccaacttc caatccggca cctccgcgcc
     1441 gattgcatcg atgacgccga ccacttcgtg ccccggcacg cgcggatatt gaatgcccgg
     1501 gaaaacaccg agcttggtga tcgaatcgct gtggcagata ccgcaggcct gcaccttgat
     1561 gcgcac
//
LOCUS       DASWZT010000531         2620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_81855, whole genome shotgun sequence.
ACCESSION   DASWZT010000531 DASWZT010000000
VERSION     DASWZT010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81855"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VGE92_15115"
     CDS             <1..750
                     /locus_tag="VGE92_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY1315210.1"
                     /translation="GSDPGSMVTRARRAPGGYKLSGAKTWISNSPIADIFIVWAKTDD
                     DIIRGFILEKGWRGLAAPVIHGKVGLRASTTGQILMDEVFVPEENLLPGVAGLKGPFT
                     CLDSARYGISWGALGAAESCWHTARQYVLERKQFGRPLAANQLVQKKLVDMQTEIALA
                     LQGCLRLGRMKDQGIASVEITSLLKRNSCGKALDVARAARDMLGGNGISDEFGVIRHL
                     VNLEVVNTYEGTHDIHALILGRAQTGIPAFT"
     gene            complement(843..1493)
                     /locus_tag="VGE92_15120"
     CDS             complement(843..1493)
                     /locus_tag="VGE92_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine utilization protein"
                     /protein_id="HEY1315211.1"
                     /translation="MNKSLTVGLVSLLSALPGHGAELIVQVQDHGKPVADAVVLATPA
                     DPKNLLRAKPPADAVDQVDKQFVPYVKPVLVGSKVRFPNSDNVRHQVYSFSSAKKFEL
                     PLYAGSTAAPVIFDKPGVVVLGCNIHDWMVGYVYVSDTPFFAKTAAAGTATLSDLPPG
                     EYTVRVWHPSMAEHAEGATTRRLTLSADAPVNVEWALSLKPIFRVPRVSTAGSQVY"
     gene            complement(1495..2382)
                     /locus_tag="VGE92_15125"
     CDS             complement(1495..2382)
                     /locus_tag="VGE92_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315212.1"
                     /translation="MGSVRCHTSLGAIAALLVGAMPYAVRSDWTLATEAALRHDSNVG
                     NSGSASDVVGDSTLSARLSLLDLFPLGESYSLTVGGNLRGELFRTLTGLNSASVEGQL
                     SLKRKWGLGAFAPWARVGLSVGRSSYDDSYRNAWDYRATVATGWRIDERLNFWADYAY
                     DRVAATPQMEEVPGLSADAYSQVSDSLTLSGEYALSERTFVNLGLLGRQGDVVSTTEG
                     NAQILTAARALAEDPAFGPEAYAYRVTGTSYGIRIGIHFSPTPHGLLGCGFERLITHA
                     YGGADYAKSIPEITWDYRF"
BASE COUNT          544 a          809 c          796 g          471 t
ORIGIN      
        1 ggatcggacc ccggcagcat ggtcacccgg gcgcgccgcg ctcccggcgg ctacaagctc
       61 tccggcgcga aaacatggat atccaacagc ccgatcgccg acatattcat cgtgtgggca
      121 aaaaccgacg acgacatcat ccgcggcttc attctggaga agggctggag gggtctcgcg
      181 gcgccggtga ttcacggcaa ggtggggctg cgcgcatcga ccacgggaca aattctcatg
      241 gatgaagtgt tcgtgcccga ggagaatcta ttgccgggag ttgcgggatt gaaaggcccg
      301 ttcacctgcc tagactcggc gcgttacggc atctcctggg gcgcgcttgg cgcggccgag
      361 agctgttggc acacggcgcg ccagtatgtg ctcgagcgaa agcaattcgg acgcccgctg
      421 gcagccaacc aactggtgca aaaaaagctg gtcgatatgc agaccgaaat cgctctggcg
      481 ctgcagggat gcctgcgact cggccgcatg aaggatcaag ggattgcctc ggttgaaatc
      541 acctccctgc tgaagcgcaa ttcctgcggc aaggcgctcg atgtggcgcg tgcggcgcga
      601 gacatgctcg gcggcaacgg catctcggac gaattcggcg tgattcggca tctcgtgaat
      661 ctcgaggtcg tgaacaccta cgagggcacc cacgatattc acgccctgat tctcggcaga
      721 gctcagaccg gaatacccgc cttcacataa tggccggttg gggcggacgg ttgtcggatc
      781 agttcaatac gatgaacgcg ggggctctgg cgccgccgat gatcacaacc ggcggcagta
      841 ttctagtaga cctgagaacc cgcggtcgat acgcggggga cgcgaaagat cggtttgaga
      901 ctgagcgccc actccacatt cacgggcgca tcagcggaca acgtcaggcg tcgagtcgtc
      961 gcgccctcgg catgttccgc catgctggga tgccagacac gcacagtgta ttcgcccggc
     1021 ggcagatcgc tcaaggtcgc ggttcccgcg gccgcggtct tcgcaaagaa gggcgtgtcc
     1081 gacacgtaga cgtatcccac catccagtcg tgaatattgc agcccaagac cacgactccg
     1141 ggtttgtcga atatcaccgg cgccgccgtg ctgccggcat acaagggcaa ctcgaatttc
     1201 ttggcggatg aaaatgaata gacctggtgc cggacgttgt cgctattcgg aaatcgcact
     1261 tttgatccca caagcaccgg cttcacgtaa ggcacgaatt gtttgtcgac ctgatcgacg
     1321 gcatccgcgg gcggtttggc gcgcagcaga tttttggggt ccgccggcgt cgcgagcact
     1381 acggcgtcgg cgaccggctt gccatgatcc tgaacctgga cgatcaattc ggcgccgtgt
     1441 ccgggcaggg cgctcaacag cgacacaagg cctacggtga gcgatttgtt catcttaaaa
     1501 gcggtagtcc caagtgattt cgggaatcga cttcgcataa tcggcgccgc cataggcatg
     1561 cgtgatcaag cgctcgaatc cgcagcccag caagccatgc ggggtgggcg agaagtgaat
     1621 gccgattcga attccataag aggtgcccgt gacgcggtaa gcgtaggcct cgggtccaaa
     1681 tgcagggtcc tcggcgagcg cgcgcgcagc cgttaaaatc tgcgcgttgc cttccgtggt
     1741 cgacaccaca tcgccctggc ggccaagcaa acctaagttc acgaaggtcc gttcgctcaa
     1801 cgcatactcg ccactcaacg tgagactgtc gctcacttga ctgtaggcgt ctgcggataa
     1861 gcccgggact tcttccattt gcggagtcgc tgccacgcga tcgtacgcat agtctgccca
     1921 gaagtttaat cgctcatcga tgcgccagcc ggtggcgaca gtcgcccgat agtcccacgc
     1981 gtttcggtag ctgtcgtcgt aactcgatcg gccgacggat agtccaacac gcgcccacgg
     2041 tgcaaaggca cccagccccc actttctctt gagcgacaac tgcccctcta cagacgcgct
     2101 gttcaagccg gtgagggtgc ggaacaattc gccgcgaaga ttgccgccga ccgtcaaact
     2161 gtagctctca ccgagcggga aaagatccaa taaggacagc cgcgcgctca gcgtcgaatc
     2221 gccgacgaca tcggacgccg atccgctgtt tccgacattg ctatcgtgtc tgagggccgc
     2281 ctcggttgcg agcgtccaat cgctgcgcac cgcgtagggc atggcgccca ccaggagagc
     2341 tgcaatcgcg ccgagagacg tgtggcaccg cacgctgccc attatgcgcg ccgccacggg
     2401 agtcgcgccc ggccgtcgcc gcgacaccga caggtggatg ccaaatcatt gcgactgccg
     2461 taggtcaccc tgggtaatca gacaggcatt gcggctgccg gtggcgtaga tccacaacca
     2521 gctcagggcc acggccaatc gattgcgcac gccaatcaag aagtaaatat gcgcaagccc
     2581 ccaaatccac caagcgagcc agccgcgcac cttgatgcgc
//
LOCUS       DASWZT010000532         2270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_14340, whole genome shotgun sequence.
ACCESSION   DASWZT010000532 DASWZT010000000
VERSION     DASWZT010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14340"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VGE92_15130"
     CDS             complement(<1..497)
                     /locus_tag="VGE92_15130"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY1315213.1"
                     /translation="MTGPVASAGQRLKAERERRGLSAQKAADELLLDDWVIVALEADD
                     YQRVGAPVFAKGHLKRYAAFLGLPADDMLAAYESSRVPAPKPPDETASVRMLSEAPAV
                     INLSQSQMIGTAAAILLLAAVLWWKPWQQRGARTAVRASTPASVPASNSAEPVPQPQP
                     TSKSKP"
     gene            complement(494..1252)
                     /gene="pilW"
                     /locus_tag="VGE92_15135"
     CDS             complement(494..1252)
                     /gene="pilW"
                     /locus_tag="VGE92_15135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEY1315214.1"
                     /translation="MKPSAATMFAMILGCCLAACSNNSGFKKASPAVTAATNMQLAIE
                     YLRLGKLAAARDFIEKALQEDPGNADVQMTAGLVYERLTDVKRAERAYSAGYRLGKGD
                     PNIQNTYAGFLCRTGKAVEGEKLFEQVARSPLYQTPEVAWVNAGVCVRSTGDLVDAER
                     YFNQALAIRPNLPEALLQLGNIELDRGDAGQARDAVVRYLASNPPSSEVLWLGFRAER
                     KLGDAPAAAAYARRVQAEFPNSEQAQMLRSGVDR"
     gene            complement(1249..>2270)
                     /gene="rlmN"
                     /locus_tag="VGE92_15140"
     CDS             complement(1249..>2270)
                     /gene="rlmN"
                     /locus_tag="VGE92_15140"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY1315215.1"
                     /translation="AADFSTMTDLARDFRTQLDAVATIKVPEIVTQQVSSDGTRKWML
                     RMDEVQGIETVYIPEPDRGTLCISSQVGCAMDCTFCSTAQQGFNRNLSVAEIVGQVWL
                     AHRELLESRPGHGDGRSGDARANITNIVFMGMGEPLANYRNVVPAMRIFLDDLGYDLS
                     RRRVTLSTSGLVPQIYKLAEECNVALAVSLHAPNDKLRDELVPINRKHPIDELLNACW
                     HYIDKQNGRSVTFEYVMLAGVNDKPEHARELARRLGGKPAKLNLIPFNVFPGTRYRRS
                     PAETIVKFRDILNERGVIATIRRTRGDDIDAACGQLAGRVTDRTLVRLGSKLAPATRT
                     PAEIR"
BASE COUNT          407 a          738 c          682 g          443 t
ORIGIN      
        1 ggtttcgact tcgacgtcgg ctgcggctgc ggcaccggct cggcagagtt ggaggccgga
       61 acgctcgccg gcgtggatgc acggaccgct gtcctggcac cgcgctgctg ccacggcttc
      121 caccacagca ccgccgccaa cagcaagatc gccgccgcgg tgccgatcat ttgggactgg
      181 gatagattga tcaccgccgg cgcttcgctc agcatcctga cactggccgt ttcgtccggg
      241 ggctttggcg ccggcacgcg cgacgactcg taggccgcca acatgtcatc ggcgggcagc
      301 cccaagaacg ccgcatagcg tttcaaatga cccttggcga acaccggcgc ccccactcgc
      361 tgataatcgt cggcctcgag tgcgacgatg acccagtcat caagcagtaa ttcatcggct
      421 gccttttgcg cactcaatcc gcgcctttcg cgctcagcct tgagccgttg cccggcggat
      481 gccacgggac cggtcatcga tcgacgccgg agcgcagcat ttgcgcctgc tcggaattcg
      541 gaaactcagc ttgcacgcgc cgcgcatacg ccgccgctgc gggagcgtcg cccagcttgc
      601 gctcggcgcg aaatcccagc caaaggacct ccgaggaggg cggattcgac gccaagtagc
      661 gcaccaccgc atcgcgcgcc tgcccggcgt cgccccgatc caattcaata ttcccgagtt
      721 gcagcaacgc ttcgggcaaa ttcggacgaa tcgccaatgc ctgattgaaa tagcgctccg
      781 catccaccaa atcgccggtg ctgcgcacgc aaacacccgc attgacccat gccacttcgg
      841 gtgtttgata caagggactg cgcgcaacct gctcgaacag cttctcgccc tccactgctt
      901 tgccggtccg gcacaggaat cccgcatagg tgttttgaat attcggatcg cccttgccca
      961 accgataccc ggccgaatag gcgcgctccg cccgtttcac gtcggtcagc cgctcgtaca
     1021 ccagtcccgc cgtcatctgc acgtcggcgt tgcccgggtc ttcttggagc gccttctcga
     1081 tgaaatcccg cgcggcggcc aatttgccga ggcgtaagta ctcgatcgca agctgcatat
     1141 tggtcgcggc ggtcacggcc ggcgacgcct ttttgaaacc ggaattgttc gagcaggccg
     1201 ccaagcagca gccaagaatc atcgcgaaca ttgtcgcggc actgggtttc atcgaatctc
     1261 cgccggagtg cgtgttgcag gagccagttt gctgccgagc cgcaccagcg tgcgatccgt
     1321 gacgcgcccc gccaactggc cgcacgccgc atcgatgtca tcgccgcgcg tcctgcgaat
     1381 cgtggcgatg acgcctcgtt cgttgagtat gtcgcgaaac ttgacgatgg tctccgcagg
     1441 cgagcgacgg tagcgcgtgc ccggaaatac attgaatgga atcagattga gcttggcagg
     1501 tttgccgccg agccgccggg cgagttcgcg cgcgtgctcg ggtttgtcgt tgacaccggc
     1561 cagcatgacg tattcgaaag tcacgctgcg gccgttttgc ttgtcgatgt aatgccagca
     1621 agcgttcagc agttcgtcga tcggatgctt gcgattgatg ggcacgagct cgtcgcgcag
     1681 cttgtcgttc ggggcatgca gcgacacggc cagcgcgacg ttgcattctt ccgccagctt
     1741 gtagatctgc ggcaccaatc ccgaagtgct caaggtgacg cgccgccgcg ataagtcata
     1801 gcccaaatcg tcgagaaata ttcgcatcgc cggcacgaca ttgcggtagt tggcgagcgg
     1861 ttcgcccatt cccatgaaca cgatgttggt gatgttggcg cgcgcgtcgc cgctgcgccc
     1921 atcgccgtgg ccggggcgcg attcgagcag ctcgcggtgc gccagccaga cctggccgac
     1981 gatttccgcg acgctcaagt tacgattgaa accttgttgg gcggtcgagc agaaggtgca
     2041 atccatggca cagccgacct ggctcgagat gcataaggtg ccgcgatcgg gctcaggaat
     2101 gtagaccgtt tcgattccct gcacttcgtc catgcgcaac atccatttgc gcgtgccatc
     2161 gcttgatacc tgttgggtga cgatttccgg tactttgatc gtcgcgacgg cgtcgagctg
     2221 cgtgcgaaaa tcccgcgcga gatcggtcat cgtcgagaag tccgccgcgc
//
LOCUS       DASWZT010000533         2783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_23914, whole genome shotgun sequence.
ACCESSION   DASWZT010000533 DASWZT010000000
VERSION     DASWZT010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23914"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VGE92_15145"
     CDS             complement(<1..858)
                     /locus_tag="VGE92_15145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HEY1315216.1"
                     /translation="MSLTSAMLTGVAALDANSQAMSITSSNIANVNTVGYKDSEADFS
                     TFLTSSYGSSINEASVLVNSQQNVTQQGVLQAASSTTDLGIQGNGFFVTSQSPTSTSN
                     LYYTRAGDFTPDSGGDLVNGSGYYLLGYPITTAGGATTTGTALQPINVSNLEGSAEPT
                     TQMVLQANLESSATVDSTYTPGDMTNGVVTPDFTRTINVYDSQGGSEPLDVSFIKTGA
                     NTWSYEVSYQGSAANIGGATNPIATGTMTFNADGTLANANSPTTPATGNVSVTIPFAA
                     ASGLTPQAIS"
     gene            complement(946..1620)
                     /locus_tag="VGE92_15150"
     CDS             complement(946..1620)
                     /locus_tag="VGE92_15150"
                     /inference="COORDINATES: protein motif:HMM:NF025230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HEY1315217.1"
                     /translation="MTTSGVNPAAAGAAASGSSDSATQQLTGNMDTFLQLLTTQLQNQ
                     DPMNPMDSDQFTQQLVEYSQVEQQINTNTNLQTLISLQQGGAASGAVSYLGKTVSVTN
                     GNASLTDGAASWNYTLAAPSSDTTLTVTNASGQTVYTGAGSTASGSNTFDWNGQDTSG
                     NQLPDGVYTLAVTAAASDGSAVQTSVSSTGVVSEVNMSGSTPELIIGSMAVPISQVAT
                     VQNSPS"
     gene            complement(1636..>2783)
                     /locus_tag="VGE92_15155"
     CDS             complement(1636..>2783)
                     /locus_tag="VGE92_15155"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEY1315218.1"
                     /translation="YEPTDPTPAQNDGTPGVTGTSPGIVPPDPGQVVNQNTVDAPTGE
                     QSSKSSDTNGNAPDQVPQAQAALQDALLDTLASGAPTPTADPTPKIVATSQPAAETSN
                     TTQLNALMQSLQPVAHRETSAPAALAQTPASQSDANQAAQPASAQNQGSDSNNSSDGH
                     SHADQTRNATESNSPQSTQQSPDSANATVVPLPSTASNSGTIANPVVDNTGGISAAVA
                     AQNSGTSSTTLHVAPASQANVQAAPPDLNALAINIAAKSQAGAKQFDISLEPAELGRV
                     EVRLTVDNAGNAQAHLVAERPETLQMLQRDSGTLSHALKESGVQLGDNGLQFSLKGQE
                     RQGDGAQQGASRARPLALSAVTSAQNILPISSSYGLAFSQAGIDIRV"
BASE COUNT          425 a          809 c          927 g          622 t
ORIGIN      
        1 ggagatcgct tgcggggtga ggcccgacgc cgccgcgaac ggaatcgtga cggagacatt
       61 tcccgtcgcc ggggtcgtcg gcgaattggc attggccaac gtgccgtcgg cgttgaacgt
      121 catggtgccg gtcgcgatcg gattggtcgc cccgccgata ttggcggccg atccctgata
      181 gctcacttca tacgaccagg tgttggcgcc tgtcttgatg aacgacacat ccagaggctc
      241 cgagccgccc tggctgtcat agacgttgat ggtgcgcgtg aagtccggcg taacgacacc
      301 gttggtcatg tcgcccggtg tgtaggtcga atcgacggtc gcgctcgatt cgagattggc
      361 ctgcagcacc atctgcgtag tcggttctgc cgagccttcg aggttgctga cattgatcgg
      421 ctgcagcgcc gttccggtcg tggttgcgcc gcctgccgtg gtgatcggat agccgagcag
      481 gtaataaccg gagccgttga cgaggtcgcc gccggagtcg ggcgtaaagt cgccggcgcg
      541 cgtataatag agattgctcg tcgaggtggg cgactggctc gtcacgaaga atccgttgcc
      601 ctggattccg agatcggtcg tcgaggaagc cgcctgcagg actccctgct gcgtcacgtt
      661 ctgctgcgag ttcaccagca cgctggcttc gttgatgctc gagccgtagg acgacgtcag
      721 gaacgtcgag aaatcggctt cgctgtcttt gtagccgacg gtgttgacgt tcgcgatatt
      781 ggacgatgtg atgctcatcg cctggctgtt ggcgtcaagg gctgccacgc ccgtaagcat
      841 tgcggatgta aggctcattt gtctcttcct ttcggtgccg cctgcgcttc gcgcttgcgt
      901 gcccgatggg tttcagggcg ccggttctcg gccaccgcct ctacgtcaac ttggtgagtt
      961 ttgaacggtc gccacctgcg agatgggaac cgccatcgat ccgatgatca gctcaggcgt
     1021 gcttcccgac atattcactt cgctcaccac gcccgtgctg ctcacgctcg tctgcacggc
     1081 ggagccgtcg ctcgcggccg cggtcacggc cagggtgtaa acgccgtccg ggagttggtt
     1141 gccgctggtg tcctggccgt tccaatcgaa cgtgttgctg ccggacgccg tcgaacctgc
     1201 gccggtgtag acggtctggc cgcttgcgtt ggtaacagtg agtgtcgtgt cggacgacgg
     1261 tgcggcgagc gtgtagttcc aactggccgc gccgtcggtc agtgaggcgt tgccattggt
     1321 gacggacacg gtcttgccca gatagctgac ggcacccgaa gcagccccac cctgctgcag
     1381 cgaaatgagc gtctgcaggt tcgtgttggt gttgatctgc tgctcgacct gcgaatattc
     1441 gacaagctgc tgcgtgaact ggtccgaatc catcgggttc atcgggtcct ggttctgcag
     1501 ctgcgtcgtg agcaactgca ggaacgtgtc catgttccct gtgagctgtt gggtcgcgct
     1561 gtcggaactg cccgatgctg cggcgcctgc tgctgccgga ttgactccgc tcgttgtcat
     1621 gacttgaagc ctttcctaaa cgcgaatgtc gatgccggcc tgcgaaaatg cgaggccgta
     1681 actggatgag atcggaagga tgttttgcgc cgaggtcacg gccgacagtg cgagcggcct
     1741 ggcgcgggac gcaccctgct gcgcgccgtc gccttgccgt tcctggcctt tgagagagaa
     1801 ttgcaatcca ttgtcgccga gttgcacgcc ggactctttc agcgcgtgag acagcgtccc
     1861 cgaatcgcgc tgcagcatct gcaacgtttc gggccgctcg gccacgagat gcgcttgtgc
     1921 gttgcccgca ttatcgaccg tcaggcgcac ttcgactcgg ccgagttcgg ccggttcgag
     1981 gctgatgtcg aactgtttcg caccggcctg cgacttggcg gcgatattga tcgcgagcgc
     2041 attgagatcg ggtggagcgg cctggacatt cgcttgcgac gcaggcgcga catgcaacgt
     2101 cgtgctgctt gtgcccgaat tctgggccgc gaccgctgcc gaaattccac ccgtgttgtc
     2161 caccacaggg tttgcgatcg tgccgctatt ggaggcagtg gaaggcagcg gcaccacggt
     2221 cgcgttcgcg ctgtcgggcg actgttgcgt ggattgcggg gaattgctct cggtcgcatt
     2281 gcgagtctga tcggcgtggc tgtgaccgtc actggaatta ttgctgtcgc tgccctgatt
     2341 ctgtgcgctt gcgggttgcg cggcctgatt tgcatcggac tgcgaagccg gtgtttgcgc
     2401 cagggccgcc ggtgcggatg tctcgcgatg cgcaacaggc tggagcgact gcatcaacgc
     2461 attcaattgc gtggtgttgg aagtttcggc cgccggctgc gaggttgcaa caatctttgg
     2521 cgtggggtcg gcagtcggcg tcggggcacc ggaagccaat gtgtccagca aagcatcctg
     2581 cagtgctgcc tgcgcttgcg gtacctgatc cggagcgttg ccgttcgtat ccgatgactt
     2641 cgaagattgc tcgcccgtcg gcgcgtcgac cgtattttgg ttcacgacct ggccgggatc
     2701 aggtgggacg atccccggcg aagtgcccgt aacacccgga gtgccgtcgt tttgcgccgg
     2761 cgtcggatcg gtcggttcgt aaa
//
LOCUS       DASWZT010000534         3337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_52733, whole genome shotgun sequence.
ACCESSION   DASWZT010000534 DASWZT010000000
VERSION     DASWZT010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52733"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /gene="lpxB"
                     /locus_tag="VGE92_15160"
     CDS             <1..1006
                     /gene="lpxB"
                     /locus_tag="VGE92_15160"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEY1315219.1"
                     /translation="AVMGLFEIVRDLPRLVRLQARIRGRFLSVRPDVFVGIDAPEFNL
                     RLARDLRAAGIPTVQYVSPQVWAWRQGRARSIHESVDLVLCLLPFEKRFYDEHGMRAE
                     FVGHPLADAIPLIPDRRAARIALELELDAQVVALLPGSRRGEVQRLAADFAATARWLA
                     AQRPQLRFIAPMASTVTRDSFAQALAQHAPGLAVKLIDGQAQTALIAADVGLIASGTA
                     TLEAALCKRPMVVVYRLGTMSAWVLRRLNLVKSRFFAQPNLLADRRVVGEYFQEEIVP
                     ESIGAELLMWLDDAERRSALEREFSRIHADLRRDAGARAARAIEALVNSRRQAAAEPQ
                     "
     gene            1003..1593
                     /locus_tag="VGE92_15165"
     CDS             1003..1593
                     /locus_tag="VGE92_15165"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003098580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEY1315220.1"
                     /translation="MRRRVAGIDEAGRGPLAGPVVAAAVILRPGRPIAGVADSKVLSA
                     EERFRLSALIRREALCFGIGWADRAEIDALNILQATFLAMRRALLAMTVTPDHVLVDG
                     NRLPQLGGLGKTLTARAIVGGDATVPAISAASILAKTARDIYMNQMDMLYPAYSFASH
                     KGYCTQDHQRLLDLHGPCPLHRRSFAPVRVALKVNV"
     gene            1590..>3337
                     /gene="dnaE"
                     /locus_tag="VGE92_15170"
     CDS             1590..>3337
                     /gene="dnaE"
                     /locus_tag="VGE92_15170"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY1315221.1"
                     /translation="MSQFVHLRLHTEYSLVDGIVRVPELMAAVSAAGMPAVALTDQSN
                     LFAMVKFYKEALAAGIKPLIGVDMWIRATGERAAPSRVVFLCQNLQGYRHLTQLVTRS
                     FLEGQQRGAPMLDRGWLGSDILEGLIVLSGGTEGDIGQAIGRGRDDEAARCLNRWQEL
                     CGDRFYIEVQRTGRAGEEIYADAVLELAQEHGVAALATNDVRFLTRAEFEAHEARVCI
                     HDGTQLADASRARRFSEEQYLKSPAEMRELFKDVPELLDNTVEVAKRCSLEIRLGSSM
                     LPAYPVPSGSNTEDYLRDEAVRGLESRAVSRPAYQARLDLELGVICSMGFAGYFLIVA
                     DFIRWARSNGVPVGPGRGSGAGSLVAFALGITDLDPIEHDLLFERFLNPERVSMPDFD
                     VDFCMEGRDRVIEYVANKYGRERVSQIITYGTLAAKAVVRDVGRVLGHNYGYVDRIAK
                     LIPFEIGMTLDKALEQEEELKRLYDGDAEIHELIDLARTLEGLARNAGTHAGGVVIAP
                     SVLTDFTPLYCEEGSTTPVTQFDKDDVEAAGLVKFDFLGLRTLTIIDWAVRDINALRA
                     AAGEPALVMSALPMDDA"
BASE COUNT          580 a          999 c         1116 g          642 t
ORIGIN      
        1 cgccgtgatg gggttgttcg aaatcgtgcg cgatctgccg cgtttggtgc gtctgcaggc
       61 gcgcattcgc ggcagattct tgagcgtcag gccggacgtg ttcgtcggca tcgacgcgcc
      121 ggaattcaat ctgcggctgg cgcgcgactt gcgcgccgcc ggtattccca cggtgcaata
      181 cgtcagcccc caggtgtggg cctggcggca gggccgggcg cgcagcatcc atgaatccgt
      241 ggatctggtg ctatgtctgc tgccgttcga gaagcgcttc tacgacgaac acggcatgcg
      301 cgccgagttt gtcggtcatc cgctcgccga tgccatcccg ttgatacccg accggcgcgc
      361 cgcgcgcatt gctctcgagc tggaactcga cgcgcaggta gtcgcgctct tgcccggcag
      421 ccggcgcggc gaagtccaac gattggcagc ggattttgcc gcgactgcgc gctggctggc
      481 ggcacaacgg ccgcaactca gattcattgc gcccatggcg agcaccgtga cgcgcgacag
      541 tttcgcgcag gcgctcgcgc agcacgcgcc cggcctcgca gtcaagctca tcgacggtca
      601 agcgcaaact gcgctcatcg ccgccgacgt gggtttgata gcatccggaa cggcgacgct
      661 cgaggcggcg ctgtgcaaac gtcccatggt cgtggtttat cgcttgggga cgatgagcgc
      721 ctgggtgctg cggcgcctga atctggtgaa atccaggttt ttcgcacaac caaatctact
      781 tgcagatcgg cgcgtcgtcg gcgagtattt tcaagaggaa attgtccccg agtcgatcgg
      841 agcagagctc ttgatgtggc tagacgatgc cgaacggcgc agtgccttgg agcgggagtt
      901 ctcgagaatt cacgccgact tgcgccgcga tgcgggagcg cgcgcggcgc gggcgatcga
      961 agcattggtg aattcgcgcc ggcaggctgc ggcagagcct cagtgagacg ccgcgtagcc
     1021 ggtatcgatg aggcgggccg cggaccgctg gcgggtccgg tggtcgcggc agcggtgatt
     1081 ctgcgcccgg ggcgtccgat cgccggcgtc gccgactcga aggtattgag cgcagaagaa
     1141 aggtttcgtt tgagtgcgct catccgccgc gaagcgctct gcttcggcat cggttgggct
     1201 gatcgggccg aaatcgatgc actcaacatc ttgcaggcca cgtttctcgc gatgcggcgc
     1261 gcgttgctgg caatgaccgt gacgcccgat cacgtgctgg tggatggcaa tcgattgccg
     1321 cagctgggtg gcttgggcaa gaccttgacc gcccgtgcga tagtgggcgg cgatgcgacg
     1381 gtccccgcca tcagcgccgc ctccatcctc gccaagacag ctcgggatat ctatatgaat
     1441 cagatggata tgctttatcc cgcatattcc ttcgcaagtc acaagggata ctgcacgcag
     1501 gaccaccagc gactgctcga cttgcatggg ccgtgtccct tgcatcggcg ctcatttgcc
     1561 ccggtcaggg tcgcgctcaa ggtgaacgtg tgagccagtt cgttcatttg cgattgcaca
     1621 ccgagtactc cctggtggat gggatcgtgc gagtgccgga gttgatggcg gcggtttcgg
     1681 cggcgggcat gccggccgtc gcgttgaccg accagagcaa tttgtttgcg atggtgaagt
     1741 tctacaaaga agccttggcc gccggcatca aacctctgat cggcgtcgac atgtggattc
     1801 gcgcaacggg cgagcgtgcg gcgccatcgc gggtggtgtt tctctgccaa aaccttcagg
     1861 gataccggca cctcacgcaa ctcgtgacgc gctcgttcct cgagggtcag caacgcggcg
     1921 cgcccatgct cgaccgcggc tggctgggaa gcgatattct cgagggattg atcgtgctgt
     1981 ccggcgggac cgaaggggat atcggccagg cgatcggccg cggccgcgac gatgaggcgg
     2041 cgcgctgcct gaatcgctgg caagaattgt gcggcgatcg attctatatc gaggtgcagc
     2101 gtaccggccg agcgggcgaa gagatctatg cggatgcggt gctcgaactg gcgcaggaac
     2161 acggcgtggc ggcgctcgcg accaacgatg tgcgcttctt gacgcgcgcc gagttcgagg
     2221 cccatgaggc gcgggtttgc atccacgatg gaacccagct tgccgacgca agccgggcgc
     2281 ggcgcttctc cgaggagcaa tacctcaagt ctcccgccga gatgcgcgag ctgtttaaag
     2341 atgtgccgga gctgcttgat aacactgttg aagttgccaa gcgatgctcg ttggaaattc
     2401 gtctcggcag ttccatgctg cccgcctatc ccgtaccgtc gggcagcaat accgaggatt
     2461 atttgcgcga tgaggcggtg cgcggcctcg agtcgcgcgc agtttcaaga cccgcgtacc
     2521 aggcgcgttt ggatctcgag ctgggtgtga tctgctccat gggattcgcc ggatacttcc
     2581 tgatcgtcgc cgatttcatc cgctgggcgc gcagcaacgg cgtgcccgtc ggtcccggcc
     2641 gcggctccgg cgcaggatcg ctggtggcct tcgcactcgg catcacggat ttggacccga
     2701 tcgaacacga tctgctgttc gagcgcttcc tgaatcctga gcgcgtttcc atgccggatt
     2761 tcgacgttga tttctgcatg gaaggccgcg atcgcgtgat cgagtacgtc gcaaacaagt
     2821 acggccgcga acgggtttcg cagatcatca cctatgggac cttggcggcg aaggcggtgg
     2881 tacgcgacgt cgggcgggtg cttggccaca actacggcta cgtcgacagg atcgcgaagc
     2941 tgattccgtt cgagatcggc atgacactcg acaaggcgct cgaacaagaa gaggaactca
     3001 agcggctgta tgacggcgat gccgagattc acgagctgat cgatctcgcg cggacactcg
     3061 agggactcgc ccgcaacgcc ggcacgcatg cgggcggggt ggtcatcgca ccgtcggtgc
     3121 tgacggattt cacgccgctg tactgcgaag agggcagcac cacacccgtg acccaattcg
     3181 acaaggacga cgtcgaagct gccggcctgg tgaaattcga tttcctggga ctgcgtacgc
     3241 tcacgatcat cgattgggcg gtgcgcgaca tcaacgccct gcgcgccgca gcgggcgagc
     3301 ccgcgctggt catgagcgca ctgcccatgg atgacgc
//
LOCUS       DASWZT010000535         2403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_76811, whole genome shotgun sequence.
ACCESSION   DASWZT010000535 DASWZT010000000
VERSION     DASWZT010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76811"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VGE92_15175"
     CDS             <1..906
                     /locus_tag="VGE92_15175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1315222.1"
                     /translation="ANAYAQHGLAYYPKLLCAVPFTPVRGPRLLIAPHTDVKNATDLL
                     IKSALNFTRSEKLSSWHILFPTDDCAQRLKSAGLILRRDCQFHWFNQNYESFDAFLAT
                     FTAEKRKKAKRERRRVAEAGIEFDTLHGGDMNDALWDIVYPFYADTFYRHGHDPYLNL
                     DFFKRIAASMPDRLMLKVARIGSERIAVAIFFVSEDALFGRYWGAGGNYHSLHFETCY
                     YQGIEYCIEHKLQRFEPGTQGEHKVPRGFVPTLMSSAHFVGDPRFAAAIRDFAAREAR
                     GVDHYAAAVNEHVPYHRAPDEELQL"
     gene            921..1616
                     /gene="aat"
                     /locus_tag="VGE92_15180"
     CDS             921..1616
                     /gene="aat"
                     /locus_tag="VGE92_15180"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEY1315223.1"
                     /translation="MGEQTDPDWFPPLENALREPPGLLAAGGDLSPARLLAAYERGVF
                     PWYSAQQPILWWSPDPRMILYPDEFKCSRSLRKTLRNGPYTTRIDSAFGATIRACAAP
                     RRGGPDTWLNNEMIASYEQLHALGFGHSIETYLAEQLVGGLYGLQLGQVFFGESMFSI
                     GRDASKVAFARLVEECRARAIQVIDCQVASAHLASLGAREVTRSQFVTLLRRYARRSP
                     SGTWAVDTAAPVP"
     gene            1678..1896
                     /gene="infA"
                     /locus_tag="VGE92_15185"
     CDS             1678..1896
                     /gene="infA"
                     /locus_tag="VGE92_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002553999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY1315224.1"
                     /translation="MSKEEAIQFEGEVVETLPNTTFRVKLKTGHVVTAHISGKMRKNY
                     IRILTGDQVTVEMTPYDLTKGRIVYRGR"
     gene            complement(1911..>2403)
                     /gene="clpA"
                     /locus_tag="VGE92_15190"
     CDS             complement(1911..>2403)
                     /gene="clpA"
                     /locus_tag="VGE92_15190"
                     /inference="COORDINATES: protein motif:HMM:NF021915.4"
                     /note="ATPase and specificity subunit of the ClpA-ClpP ATP
                     dependent serine protease; directs protease to specific
                     substrates; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEY1315225.1"
                     /translation="RHVIIIMTTNAGAFEMSRPSIGFTQSDTGADGMEAIRRLFSPEF
                     RNRLDAVIQFGSLDQDTIERVVDKLLIEAEAQLEQKRVSISVSEEARRWIAKRGYDPK
                     MGARPMARIIQEFIKRPLAEELLFGKLVNGGHVEVTLGEEGDKLKLETRAANQPALLS
                     NES"
BASE COUNT          454 a          759 c          697 g          493 t
ORIGIN      
        1 gccaatgcct atgcgcaaca tggccttgcg tactacccga aattgctctg cgcggtgccc
       61 ttcactccgg tgcgcgggcc gcgtcttctc atcgcgccgc acacggatgt caagaatgcg
      121 accgatctgc tgatcaagag cgcgctgaac ttcacgcgca gcgaaaagct ctcatcctgg
      181 catattctgt ttccgacgga cgactgcgcg cagcgcctga agtcggcggg cctgatcctg
      241 cgccgcgact gccaattcca ttggttcaac cagaactacg aatccttcga tgccttccta
      301 gcgactttca cggccgaaaa acgcaagaaa gccaagcgcg agcggcgccg cgtcgcggaa
      361 gccgggatcg agttcgacac actgcacggc ggcgacatga acgatgcgtt gtgggacatc
      421 gtatatccgt tctacgccga tacgttctac cgacacggcc acgatccgta tctcaacttg
      481 gacttcttca agcggatcgc cgcctccatg ccggatcggc tgatgctcaa ggtggcgcgc
      541 atcggtagcg aacgcatcgc ggtggcgatt tttttcgtga gcgaggatgc gctatttgga
      601 cgctactggg gtgccggcgg caattatcac agcctgcatt tcgagacttg ctactatcag
      661 ggaatcgaat actgcatcga acacaaattg cagcgctttg agcctgggac tcagggcgag
      721 cacaaagtgc ctcgagggtt cgtgccgacc ttgatgagtt cggcgcattt tgtcggcgac
      781 ccgcgctttg ccgcggcgat tcgtgacttt gcggcgcgcg aggcgcgcgg cgtggatcat
      841 tatgcggcag cggtcaacga acacgtcccc tatcatcgtg cacccgatga ggagttgcag
      901 ctctgagcag actcatttgg ttgggcgaac aaaccgaccc ggactggttt ccgcccttgg
      961 agaacgcgct gcgcgaaccg cccggcttgc tcgcggccgg cggcgacttg agtccggcgc
     1021 gtctgctggc cgcgtatgag cgcggcgttt tcccatggta ctcggcgcaa caacccattc
     1081 tttggtggtc gcccgatccg cgcatgattc tctatcccga tgaattcaag tgctcgcgca
     1141 gcctgcgcaa gaccctgcgc aatggtccct acacgacgcg tatcgactcg gcgttcggcg
     1201 cgacgatccg tgcctgcgcg gcgccgcgcc gcggcggacc cgacacctgg cttaacaacg
     1261 agatgatcgc ctcgtatgag cagttgcatg cgctcggctt cggtcactcc atcgagacct
     1321 accttgcgga gcaactggtg ggcggcctct atgggctgca gttgggccag gttttctttg
     1381 gcgaatcgat gttcagcatc gggcgcgatg cctcgaaagt ggcattcgcg cgcttggttg
     1441 aggagtgccg cgcgcgcgct atacaggtga tcgattgtca ggtggcgagc gcccaccttg
     1501 cttcgctggg tgctcgggaa gtgacgcgca gccaatttgt gacattattg cgacgctatg
     1561 ccaggcgcag cccgagcggc acctgggccg tcgacacagc ggcgcccgtg ccgtaaattg
     1621 cattaccagg gggctttatg cacaatgcgc ccggtttccc gcataccaca aggacgcatg
     1681 tcaaaagaag aggccattca attcgagggc gaggtcgtcg agaccctgcc caatacaacg
     1741 tttcgcgtca aactcaagac cggccacgtc gtaaccgctc atatttccgg caagatgcgt
     1801 aaaaactaca ttcgcatact gaccggcgac caggtcaccg tcgagatgac gccctacgat
     1861 ctgaccaagg gccgcatcgt ctaccgcggc cgctagccga gcgcttcgaa ctagctctcg
     1921 ttcgacaaca atgccggctg attcgcggcc cgagtctcga gtttgagctt gtcgccctcc
     1981 tcgcccaaag tcacctccac gtgtccgccg ttgacgagtt tgccgaacag cagctcctcg
     2041 gcgagcggtc gcttgatgaa ctcctgaatg attcgcgcca ttggccgcgc gcccatcttc
     2101 ggatcgtagc cgcgtttggc gatccagcgg cgcgcttcct cgctcaccga gatcgaaaca
     2161 cgcttctgtt cgagctgcgc ctccgcctcg atcaacagct tgtccaccac ccgctcgatc
     2221 gtatcctgat ccaggctgcc gaattgaatg accgcatcca aacggttgcg gaactccggc
     2281 gagaacagcc gccgaatcgc ttccatacca tccgcgccgg tgtccgactg cgtgaatccg
     2341 atgctcggcc ggctcatttc aaaggccccc gcattggttg tcatgatgat gatgacgtgg
     2401 cgg
//
LOCUS       DASWZT010000536         2102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_81922, whole genome shotgun sequence.
ACCESSION   DASWZT010000536 DASWZT010000000
VERSION     DASWZT010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81922"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..1507
                     /locus_tag="VGE92_15195"
     CDS             182..1507
                     /locus_tag="VGE92_15195"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY1315226.1"
                     /translation="MGLSEDRIMTQILCLTIALAGLLGTVSCAARDLVIHAGTLLDGI
                     TDSPRLAMSILIHDDKIESVVSGFVSPAGAEVIDLTTATVMPGYIDCHVHISSLLPSR
                     TNATEYWLTHTDIDRALDATLFVRQMLQQGFTSARDVGGGDETVAVRNAIRDGKIAGP
                     RLWVSLEPLGPTAGHGDPKSGIDPALSHPGWERGIVDSPEQARLRVREHRGRGADVIK
                     LMPSGGIASTGDDPRQQLMTNEEMRVAVETAHSLGMKVAAHIYPADAIENAVRAGVDS
                     VEHGSFAGAQTFALMKAHGTYLVPTLSVYEVFYAAARDRPELLTPGTAQKELANDLLP
                     KKNLPLAVKSGVKIAYGTDLGQGDHTLEFALLIANGMTPIQALDAATRNAADLIGAAD
                     RIGSIQAGRYADLVATAANPLDHPDQFKQVDFVMQGGRIYRRNGLETIP"
     gene            1504..2043
                     /locus_tag="VGE92_15200"
     CDS             1504..2043
                     /locus_tag="VGE92_15200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1315227.1"
                     /translation="MSVILRKALFADVPAIEALIARSARGLSAEYRPAQVEGALRGAF
                     GVDTQLLADQTYFIAEEDARPVGCGGWSFRSTLFGSDARAHRDASLLDPRNSAAKIRA
                     FFVDPAHARRGIGTLLLERCESEARSHGFAAVELMATLAGVKLYAARGYVGSAMVHFA
                     VGDAETIEFIPMRKVLVAP"
BASE COUNT          382 a          696 c          653 g          371 t
ORIGIN      
        1 acccaacgcc aggggccacg acttggcgaa tcccctgcgg aacaatcgga tcgaatcgtc
       61 gagcacgccg ccgatgctct gcggcgcggt tgattttatg aacacgagat cctccctgga
      121 gcggccgttg accgtcaccg agcgcagatt aacacgcggc aggacgcgct acacttaagt
      181 catggggctg tccgaggatc gaatcatgac gcaaattctg tgtttgacca tcgccctggc
      241 tgggctgttg ggcaccgtct cttgcgccgc cagggacctc gtgatccatg ccggcacgtt
      301 gctcgacggg atcacggatt caccgcgact tgcgatgtcg attctgattc atgacgacaa
      361 gatcgagtcc gtcgtgtccg gattcgtatc gccggcgggt gccgaagtca tcgatctgac
      421 gactgccacg gtgatgccgg gatacatcga ctgccatgtg cacatttcct cgctgctgcc
      481 gagccgcacc aacgccacgg aatactggtt gacgcacacg gatatcgatc gcgcgctcga
      541 cgcgacgctg ttcgttcgcc agatgctgca acaaggtttt accagcgcgc gtgacgtcgg
      601 gggcggcgac gagaccgtcg ccgtgcgcaa tgccatcagg gacggcaaaa ttgccgggcc
      661 gcggctctgg gtctccctgg agccgttggg tccgaccgcc gggcacggcg acccgaaaag
      721 cggcatcgac ccggcgctat cccatccggg ctgggagcgc ggcattgtcg actcgcccga
      781 acaggcgcgc ctgcgcgtgc gcgaacatcg cggacgcggt gccgatgtca tcaagctcat
      841 gccctcgggc ggcatcgcct ccacgggcga cgacccccgc cagcaactga tgaccaacga
      901 ggaaatgcgc gtcgcggtcg aaaccgcgca ctcgctcggc atgaaggtag ccgcgcatat
      961 ctatcccgcc gacgccatcg agaacgcggt acgcgccgga gtcgactcgg tcgaacacgg
     1021 ctcttttgcc ggcgcgcaga cctttgccct gatgaaggcg cacggcacct acctcgtgcc
     1081 gacattgagc gtctatgagg tgttttacgc ggccgcgcgc gatcggcccg agttattgac
     1141 ccccggtacg gcgcagaagg aactcgcgaa cgatttgctg ccgaagaaaa acctaccgct
     1201 cgccgtgaag tcgggcgtca aaatcgccta cggcaccgat ttgggccaag gcgatcacac
     1261 gttggaattc gcgctgctca tcgccaacgg tatgacgccc atccaggcgc tcgacgccgc
     1321 cacgcgcaat gccgccgact tgatcggtgc cgccgaccgg atcggctcca tccaggccgg
     1381 ccggtatgcg gatctcgtgg caaccgccgc caacccgctc gatcaccctg accaattcaa
     1441 gcaggtcgac ttcgtgatgc aagggggccg gatctatcgg cgcaacggcc tcgaaacgat
     1501 cccatgagcg tcatcctgcg caaagcccta ttcgccgatg tgcccgcgat agaagcgctg
     1561 attgcccgtt ccgcccgcgg cttgagcgca gagtaccggc ccgctcaggt cgaaggcgcc
     1621 ttgcgcggtg catttggcgt cgatactcag ttgctggccg accagacgta tttcattgcg
     1681 gaggaagacg cgcggccggt cgggtgcggc ggctggagct ttcgatcgac actgttcggc
     1741 agcgatgcgc gcgcccaccg cgatgcctcg ctactcgatc cgcggaacag cgccgcgaag
     1801 attcgcgcct tttttgtcga tcccgcccat gcgcgccgcg gcatcggcac gctgctgctc
     1861 gagcgctgcg agagcgaagc gcgcagccac ggcttcgccg cggtcgaact gatggccacc
     1921 ctcgccggcg tcaagctcta cgccgcgcgc ggctatgtgg gctcggccat ggtgcatttc
     1981 gccgtcggcg atgcagagac catcgaattc attccgatgc gcaaggtatt agtcgcgcca
     2041 tagtcgccgc cagccgtcga ggcccaggtc acgcaaggta cgaatgttgc ggcggtatat
     2101 ct
//
LOCUS       DASWZT010000537         7741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_67168, whole genome shotgun sequence.
ACCESSION   DASWZT010000537 DASWZT010000000
VERSION     DASWZT010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7741
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67168"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VGE92_15205"
     CDS             <1..906
                     /locus_tag="VGE92_15205"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEY1315228.1"
                     /translation="RIKILVRTLGIEEFRRRVEAEWQLIRGAGPRVDEAEITRLRSFF
                     HPPAYRQLADVEPAIGKSAAFQAWYRYNTTAHKVPGYRAVYISLKKPDVAPGDITDSQ
                     MELVADLADRYSFGEVRVTHTQNLLLADVEQSAAFELWLMLEKAGLATPNIGTLTDMI
                     CCPGLDFCSLANAGSIDVAKLINERFDNYDYLYDLGELEIKMSGCMNACGHHHVGHIG
                     ILGVDKSGEEWYQITIGGAAGVDASLGHVIGPSVKKADVAETIARLLEIYVEQRLPDE
                     RFLDTVRRLGVKPFKERVYATPIAA"
     gene            884..1393
                     /locus_tag="VGE92_15210"
     CDS             884..1393
                     /locus_tag="VGE92_15210"
                     /inference="COORDINATES: protein motif:HMM:NF017849.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HEY1315229.1"
                     /translation="MPRRLLRDGQIIVDDWSYAAELAGNPAGAVILSVDAWQREREKW
                     DGGGWRLGLVLLPAHRVEVLAADISRFELIAAEFSGPGEGRGYSQARVLREQWNFKGE
                     LRATGYVRRDQLFFMARCGFNSFELPDADLACAHTALSTFSWAYQPSNDAGLALTVPQ
                     RRPDIPASA"
     gene            complement(1350..1724)
                     /locus_tag="VGE92_15215"
     CDS             complement(1350..1724)
                     /locus_tag="VGE92_15215"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE-like domain-containing protein"
                     /protein_id="HEY1315230.1"
                     /translation="MHRFLGQVNELPVRVRDGRAFLGPIDISAAAELRAGGAGNIALI
                     RPHEIEIGSATQGWPATVVAVRVIGPIVRLELCAEGQPANEPLEAEISRERYESERFK
                     VGQKVGLRFRRWQVYPADAAAR"
     gene            complement(1704..2486)
                     /locus_tag="VGE92_15220"
                     /pseudo
     CDS             complement(1704..2486)
                     /locus_tag="VGE92_15220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00968.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
     gene            complement(2417..3295)
                     /gene="cysW"
                     /locus_tag="VGE92_15225"
     CDS             complement(2417..3295)
                     /gene="cysW"
                     /locus_tag="VGE92_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008034093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEY1315231.1"
                     /translation="MAAAEPLPAIGTRLSRWLLIAVALLFLGLFLAMPLLVVFAQALS
                     KGIGTYLLAISDPMAWAAIKLTLAVAAIAVPCNLVFGVAAAWAIAKFEFRGKSTLITL
                     IDLPFAVSPVIAGLVFILLFGSHGWFGGLLRAHHVRVVFAVPGIVLATVFVTFPFIAR
                     ELIPVMQAQGKDDEETALSLGATGWQTFWRVTLPNIRWGLLYGVLLCNARAMGEFGAV
                     SVVSGHIRGRTNTLPLHVEILYNEYNYTAAFAVASLLALLALLTLAGKSFLEWRQPEL
                     RRVTHPARDRTRRAHT"
     gene            complement(3282..4091)
                     /gene="cysT"
                     /locus_tag="VGE92_15230"
     CDS             complement(3282..4091)
                     /gene="cysT"
                     /locus_tag="VGE92_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEY1315232.1"
                     /translation="MLPGFGLTLGYTMLYLSLLVLIPLSAVFIRSLGSGWGHFWDVVT
                     DRRVLAALRLSFGASLVAASINVIFGLIVAWVLVRYPFPFKKLVDASVDLPFALPTAV
                     AGIALTALYAPAGWLGVRLLSLGVKVAFTPLGIIVALTFIGLPFVVRTLQPVLEDLDH
                     EVEQAAATLGATGLQAFVRVILPALWPALLTGFALAFARAVGEYGSVIFIAGNMPMKS
                     EIAPLLIVTKLEQYDYAGATAIAVVMLVASFAIMLGINALQHWALTRHGGR"
     gene            complement(4166..5176)
                     /locus_tag="VGE92_15235"
     CDS             complement(4166..5176)
                     /locus_tag="VGE92_15235"
                     /inference="COORDINATES: protein motif:HMM:NF008022.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY1315233.1"
                     /translation="MRREGFWIALLIAAVLTGPAAVRAATIKLLNVSYDPTRELYEEY
                     NKAFAAYWKGKTGDTVSINQSHGGSGKQARAVIDGLEADVVTLALAYDIDALALQGGL
                     IPKNWQTRLPNNSTPYTSTIVFLVRKGNPKNIRDWSDLVRPGIAVITPSPKTSGGARW
                     NYLAAWAWALNQPGGNDASAEAFVKKLYHNVPVLDSGARGATTTFGERGIGDVLLAWE
                     NEAYLAIKELGPAKFDIVAPSLSIVAEPPVSVVDQNVDEHGTRKVAEEYLKYLYSAQG
                     QEIVARHFYRPRDPAIAALHAAQFAVIPRLVTIDAVFGGWAKAQAAHFADHGTFDRIY
                     GQ"
     gene            complement(5176..6159)
                     /locus_tag="VGE92_15240"
     CDS             complement(5176..6159)
                     /locus_tag="VGE92_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012313669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY1315234.1"
                     /translation="MKFQQLRYLVAVHENGLNITAAARQLHTSQPGVSRQLKLLEEEL
                     GFQLFEREGRALTRTTDAGEEIISRASAILREMQNIRRTSAELRKADGGTLSIATTHT
                     QARYVLPEVIRAFRVKYPKVRLHLHQGTSEQIAEMVARDRVDFAIATGSEEQFPHLVR
                     LPVYRWHRAVVVPRGHPLASAGKLTLKKLAEYPIVTYVFSISGRSSLPALFETAGLSL
                     DVALTARDSDVIKTYVRIGLGVGILARLAIDPAADADLVVLDAAHLFEGHTTWIGFRR
                     SALLRAYMYEFIELLAPHLPRKLVRETEKLETQEEVNRILEPLQIPLRDTP"
     gene            6450..>7741
                     /gene="cysG"
                     /locus_tag="VGE92_15245"
     CDS             6450..>7741
                     /gene="cysG"
                     /locus_tag="VGE92_15245"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF007922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HEY1315235.1"
                     /translation="MNYFPVFFNLDGQKVLIVGGGAVALRKILLLERTGALLSVVAPQ
                     IAPEIMQRAAAGTLMLAVREFVAEDLDGARLVIVATSRDAVNRWIAKLSESRNIPVNV
                     VDDVQASRFIVPAIIDRDPVLIAVSTGGASPVLARRLRERLEALVPRSIGEFAAWLRE
                     LRASTRRQLRDVDQRRRFFETLVDGPAARRFIAGDNQGARRIAQRLLATTSTAPRASG
                     EVTLVGAGPGDPELLTLKALRALQDADVILHDRLVPAAVLDLARRDAEQICVGKAAGN
                     IGSTQQEINSLMIEQARQGKRVVRLKGGDPFVFGRGGEELQALAEADIDFSVIPGITA
                     AAGCAAYAGIPLTHRDYAQSLIFVTGHAPDEYQQPDWRALAQPGVTAVFYMGLGRLKR
                     IVEKLLEHGAEASRPAGIIAQGTTANQRVVTATLATLGA"
BASE COUNT         1502 a         2484 c         2406 g         1349 t
ORIGIN      
        1 cgtatcaaga ttctggtgcg caccctgggt atcgaggagt ttcggcggcg cgtggaagct
       61 gagtggcagc tgatacgcgg cgcgggtcct cgggtcgatg aggcggagat tacccggctg
      121 cggagcttct tccatccgcc ggcctatcgg caactggcgg acgtcgaacc tgcgatcggc
      181 aagagcgcgg cgtttcaggc ctggtaccgc tacaacacaa cggcgcacaa agtacccgga
      241 taccgcgccg tatatatatc gctcaagaag cccgacgtcg cccccggcga catcacggac
      301 tcgcagatgg agttggtcgc cgatctcgcc gatcgctaca gcttcggcga agtgcgcgtc
      361 acccacacgc aaaatttact gctcgccgac gtcgagcaga gcgcggcgtt cgagctgtgg
      421 ctgatgctcg agaaggcggg tcttgcaacg ccgaacatcg gaactttgac cgacatgatt
      481 tgttgcccgg gtctcgattt ctgcagcctt gcgaacgccg gctccatcga tgtcgcgaag
      541 ctcatcaatg agcggttcga caattacgat tatctctatg acctgggcga gctcgagatc
      601 aaaatgtccg gctgcatgaa cgcctgcgga caccaccacg tcggacacat cggcatactc
      661 ggcgtggata agagcggcga ggagtggtac caaatcacca tcggcggtgc cgcgggtgtc
      721 gatgcttcgc tcggccatgt catcggtccg tcggtgaaga aagccgacgt cgccgagacg
      781 atcgcgcgtc tgctcgagat ctacgtcgaa cagcgcctgc ccgacgagcg ctttctcgac
      841 accgtacgtc ggctcggcgt aaagccattc aaggagaggg tctatgccac gccgattgct
      901 gcgtgacgga cagatcatcg tcgacgactg gtcgtacgcg gccgaactgg ccggcaatcc
      961 ggcaggcgcc gtgatcttga gcgtggatgc gtggcaacgc gagcgcgaaa agtgggatgg
     1021 cggcggttgg cgcttgggtc tggtgctgct gcccgcgcac cgggtcgagg tcctggctgc
     1081 ggatatttcg cgtttcgagt tgatcgcagc ggagttttcc ggacctggcg aaggacgcgg
     1141 ttattcccag gcgcgcgtgc tccgcgagca gtggaacttc aaaggcgagc tgcgggcgac
     1201 gggctatgtg cgccgtgatc aattgttttt catggcgcgt tgcggtttca atagcttcga
     1261 attgccggac gcagacttgg cctgcgcgca tacggcgctg tcgacctttt cgtgggctta
     1321 ccaaccgtcc aatgacgccg ggctcgccct taccgtgccg cagcgtcggc cggatatacc
     1381 tgccagcgcc tgaaacgcag gcccactttc tgacccacct tgaagcgctc gctctcgtag
     1441 cgctcgcggc tgatttccgc ttccagcggc tcgttcgccg gctgaccctc ggcgcacagt
     1501 tccaaacgca cgatcggccc gatcacgcgc accgcgacca cggtcgccgg ccagccctgc
     1561 gtcgcggatc cgatttcaat ttcgtgcggc cgaataagcg caatgttgcc ggcgcccccg
     1621 gcgcgcaact cggcagcggc tgaaatatcg atcggaccca ggaaggcgcg gccgtcgcgc
     1681 acccgcaccg gcaattcgtt cacctgcccg aggaagcggt gcacgaacgc cgaggtcggt
     1741 acatcgtata cctcgctcgg cacgccgcac tgttcgatgc ggccgcgatt gagcaccacg
     1801 atcgtatcgg ccacctcgat cgcttcctgc tgatcgtgcg tcacgaaaat gctggtgaaa
     1861 tgcagctcgt cgtgcaagcg gcgcagccaa cggcgcagct cgtggcgcac ctggctgtcg
     1921 agcgcgccga acggctcgtc cagcaacagc acgtcggggc caaccgccag cgcgcgcgcc
     1981 agcgccacgc gttgccgctg tccgccggag agttgcgcgg gatagcgctc gcccgcccaa
     2041 ccgagctgta ccaggtcgag cagcgcgcgc actttcttgc gtatttccgc ggctggcggc
     2101 cgctcacgcc ggcgtttgac cttcaatccg aaggcgacat tctcggagac cgacatgtgg
     2161 cggaacagcg catagtgctg gaacacgaag ccgacgtttc gatcgcgaat ccgccacgat
     2221 gttgcgtcgc gtccgtcgaa cagcacctgc ccctcgtccg gggtgtccag accggcaatg
     2281 attcgcagca gtgtggtctt gccgcagccc gaaggcccga gcaacgcgat caacgcgccg
     2341 gtcggcacgt ccagatcgat gttctttacc gcggcaaatt caccgaattg cttgcctatg
     2401 ccgcgtagcg cgatgctcaa gtgtgtgccc gccgcgttcg atcgcgcgcc ggatgcgtca
     2461 cacgccgcag ttcgggctgc cgccactcca ggaagctctt gccggccaac gtcagcaatg
     2521 ccagcaatgc cagcagcgaa gccacggcga atgccgccgt atagttgtat tcgttgtaaa
     2581 ggatctcgac atgcaaaggc agggtattgg tgcggccgcg gatgtgcccg gaaaccaccg
     2641 agaccgcgcc gaactcgccc atggcgcggg cgttgcacag cagaacaccg tacaacaggc
     2701 cccagcgaat attcggcagc gtcacgcgcc aaaaggtttg ccagccggtt gcgccgagcg
     2761 acagcgcggt ctcctcgtca tccttgccct gggcttgcat caccggaatc agttcgcgag
     2821 caatgaacgg aaacgtgacg aatacggtgg ccagcacgat ccccggcacg gcaaatacca
     2881 cccgcacgtg atgagcccgc agcaatcctc cgaaccaacc atgggagccg aacagcagaa
     2941 tgaagaccag accggcgatg accggcgaca cggcaaaggg caagtcgatc agcgtgatca
     3001 aggtactctt gccgcgaaat tcaaacttgg ctatcgccca cgccgcagcc actccgaata
     3061 cgaggttgca cggcaccgcg atggcggcga cggcgagtgt cagcttgatc gccgcccagg
     3121 ccatcggatc ggaaatcgcg agtagatagg tgccgatgcc tttgcttagc gcctgcgcga
     3181 acaccaccag cagcggcatt gccagaaaca ggcccagaaa cagcagcgcg accgcgatca
     3241 gcagccagcg gctcaagcgc gtgccgattg cgggcagcgg ttcagcggcc gccatgtcgc
     3301 gtcagagccc agtgttgcag cgcgttgatg ccgagcatga tcgcaaacga ggccaccagc
     3361 atcacgacgg cgatcgcggt cgctcccgcg tagtcgtatt gctccaattt ggtgacgatc
     3421 aggagcggtg cgatctccga tttcatgggc atattgccgg cgatgaaaat gaccgagccg
     3481 tattcgccga ccgcgcgcgc aaaggccagt gcaaatccgg tgagcaacgc gggccagagg
     3541 gccggcaata tgacgcgcac gaaggcctgc agcccggtcg cgccgagggt ggccgcggct
     3601 tgctccactt catggtccaa atcctcgagt accggctgca gcgttcgtac cacgaacggc
     3661 agaccgataa acgtcaatgc cacgatgatt cccagcggcg tgaaggcgac cttgacgccg
     3721 agcgacagca gccgcacacc gagccagccg gcaggagcat acagagcggt caaggcaatt
     3781 cccgccaccg cagtcggcaa tgcaaacggc agatcgacac tggcgtcgac cagcttcttg
     3841 aacgggaacg ggtagcgcac cagcacccaa gccacgatga ggccgaaaat gacattgatc
     3901 gacgccgcga ccaatgaagc gccgaagctt aagcgtaacg ccgcgagcac tcgccggtcg
     3961 gtcaccacat cccagaaatg gccccagccg gagccaaggc tgcgaatgaa caccgccgac
     4021 agaggaatca acaccagcag cgacagatac agcatggtgt agcccagggt gagcccgaaa
     4081 ccggggagca ccggccgcgg cgcgcgccgt ggcgagggac tatcgaggtc gattgcggcg
     4141 gcgggcgttg ccaagggagc cacgcttact ggccatagat tctatcgaag gtaccatggt
     4201 ccgcgaagtg cgctgcttgc gccttcgccc agccgccgaa cacggcatcg atggtcacca
     4261 gtcgaggaat aactgcgaat tgggccgcat gcagagccgc aatcgccgga tcgcgcgggc
     4321 ggtagaaatg tctcgccaca atctcctggc cctgggcgga atacaaatac ttcagatatt
     4381 cttcggcgac tttgcgcgtc ccgtgttcat ccacgttctg atcgaccacg ctcaccggcg
     4441 gttccgccac gatgctgagc gacggagcga cgatatcgaa tttagccggt cccagttcct
     4501 tgatcgcaag ataggcttcg ttctcccagg ccagcaacac atcgccgatg ccgcgttccc
     4561 cgaaagtcgt cgtcgcgccg cgcgcccccg aatcgagcac cgggacgttg tgatacagct
     4621 tcttcacgaa ggcctcggcg gaagcgtcgt tgccgcccgg ctgattcagc gcccaagccc
     4681 aggccgcgag ataattccaa cgcgcaccac ccgaggtttt cggactcggt gtgatgaccg
     4741 cgatgcccgg ccgcaccaaa tcgctccagt cacgaatgtt cttcgggttg cctttgcgca
     4801 ccagaaagac gatcgtcgag gtatagggcg tgctgttatt gggtaggcgc gtctgccagt
     4861 tcttgggaat gaggccacct tgaagtgcca gtgcgtcgat gtcgtaggcc aacgccaggg
     4921 tcacgacatc cgcctcgagg ccatcgatta ccgcgcgtgc ctgctttccc gatccgccgt
     4981 ggctttggtt gatgctgacc gtatcgccgg tcttgccctt ccagtaggcc gcaaacgctt
     5041 tgttgtattc ctcgtagagt tcgcgcgtcg gatcgtagga gacgttcaga agtttgatgg
     5101 tcgccgcgcg caccgccgcc gggcccgtca gcacggcggc gatcagcagc gcgatccaga
     5161 aaccctcgcg ccgcatcagg gcgtgtcgcg gagcggaatt tgcagaggct cgagaattcg
     5221 attgacctcc tcctgggtct ccaatttctc cgtctcgcgc accagcttgc gcggcaggtg
     5281 cggcgccagc agctcgatga actcgtacat gtaggcccgc aacagggcgc tgcgcctgaa
     5341 cccgatccac gtcgtgtgac cttcgaacaa atgcgccgca tcgagcacga ccaaatccgc
     5401 gtcagccgcc ggatcgattg ccagacgcgc caagatcccg accccgagac cgatccgcac
     5461 ataggtcttg attacatccg agtcgcgggc cgtgagcgcg acgtcgagcg acaggccggc
     5521 ggtttcgaac agcgcgggca acgaggagcg gccggagatg ctgaatacat aggtgacgat
     5581 cgggtattcg gccagcttct tcagcgtcag cttgccggcc gacgccagcg gatggccgcg
     5641 cggtaccacc accgcacgat gccagcgata caccggcaaa cgcaccagat gcggaaattg
     5701 ctcctcggac ccggtggcaa ttgcgaagtc gacccgatcc cgggccacca tctcggcaat
     5761 ttgctcggaa gtgccctggt gcagatgcag acgtaccttg gggtacttga cgcgaaatgc
     5821 gcgaatcacc tccggcagca catatcgagc ctgcgtatgc gtggtcgcaa tcgacagggt
     5881 gccgccgtcc gccttgcgca gttccgcgga cgtgcggcga atgttctgca tttcgcgcaa
     5941 gatggcgctg gcgcgcgaaa tgatctcctc gcccgcatcg gtggtccggg tgagcgctcg
     6001 gccctcgcgt tcgaacaact gaaagccgag ttcctcctcc aaaagcttca gctgacggct
     6061 gaccccgggc tgcgaggtgt gtagctgccg cgcggccgca gtgatgttca ggccgttctc
     6121 gtggaccgct accagatagc gcaattgctg aaacttcatt ccgggagctg cccttgcgtt
     6181 gaatcgttat acgcatagga tataaggttc gccccggccg cgcttagggt cgtttactgc
     6241 acccgcgcca tcacgaattc gcatgtgctt atgcccggga gtttgtttcg cgatccgcgc
     6301 gcgccaaaaa cgcgtgctac gccaacagtt tagacctcat cggatcaaac agcgctaccg
     6361 ccaggcactg accatctact atcctaagac cgaggaatgc acaacgcgta caacatgttc
     6421 tacgataggc gccaggcggc attttccaaa tgaattattt tccggtcttt ttcaacctcg
     6481 acgggcaaaa agttctgatc gtgggcggcg gcgcggtggc cctgcgaaaa attttgctgt
     6541 tggagaggac cggcgcactg ctgagcgtgg ttgcgccgca aatcgcaccc gaaatcatgc
     6601 agcgcgccgc cgccggcacg ctcatgctcg ccgtgcgcga gttcgtcgcc gaggatctgg
     6661 atggagcgcg gctggtcatc gtcgccacct cgcgcgacgc cgtgaaccga tggatcgcga
     6721 agttgagcga gtcgcgcaac attcccgtca atgtggtcga cgacgtccaa gcgtcacgct
     6781 tcatcgttcc ggccatcatc gatcgcgatc ctgttctcat cgcggtctcc accggcggtg
     6841 catcgccggt gctggcacgg cgcttgcgcg aacgcctcga ggcactcgtg ccacgcagta
     6901 tcggcgaatt tgccgcgtgg ctgcgcgagc tgcgcgccag cacgcgccgg caactgcgcg
     6961 acgtggacca gcggcggcgt tttttcgaaa cgctggtcga cggtccggcc gcacggcgct
     7021 tcatcgcggg cgacaaccag ggcgcgcgcc gtatcgcaca gcggctgctg gcgacgacct
     7081 cgacggcacc gcgcgcgagc ggcgaggtca ccttggtggg cgcgggaccc ggcgacccgg
     7141 aactcttgac gctcaaggcg ctgcgcgcgc tgcaagacgc cgacgtgatc ctgcacgatc
     7201 gcctggtgcc cgccgcggtt ctcgatctcg cacgacgcga cgccgagcaa atttgcgtgg
     7261 gcaaagcggc cggcaatatc ggcagcacac aacaggaaat caactccctg atgatcgagc
     7321 aggcgcggca gggcaagcgg gtggtccgcc tcaagggggg cgatcccttt gtgttcgggc
     7381 gcggcgggga ggagctgcag gcgctggcgg aggctgacat cgatttttca gtgatacccg
     7441 gcattaccgc cgccgcaggc tgcgccgcgt acgccggaat cccgctgacg catcgcgact
     7501 acgcgcagag cctgatcttc gtcaccggcc atgcgccgga cgagtaccag caacccgatt
     7561 ggcgggcatt ggcacagccg ggcgtcacgg cggtgttcta catgggcctg ggtcgcctta
     7621 agcgtattgt ggagaaactg ctcgagcacg gggcagaggc gagccggccg gccggcatca
     7681 ttgcgcaagg cacgactgcc aatcaacgcg tcgtaaccgc gacgctcgcc acactcggcg
     7741 c
//
LOCUS       DASWZT010000538         1647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_28549, whole genome shotgun sequence.
ACCESSION   DASWZT010000538 DASWZT010000000
VERSION     DASWZT010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28549"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VGE92_15250"
     CDS             complement(<1..183)
                     /locus_tag="VGE92_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315236.1"
                     /translation="MTKLLGITASTPDDWKLTDLGWIFSIAIFFLGASAAVCGRWVEE
                     GGPRRAMFTSGLCWAGG"
     gene            complement(582..1478)
                     /locus_tag="VGE92_15255"
     CDS             complement(582..1478)
                     /locus_tag="VGE92_15255"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY1315237.1"
                     /translation="MIDKLEFLLALAREKHFGHAAEACGVTQPTLSAGVKQIEDTFGV
                     LLVNRGSRFQGFTPEGERVLDWARRIVGDARAMREEVHALKHGLVGRLRLAAIPTALA
                     MVETLTTPYRARHPDVQFTVYSRTSIEILALLENLEIDAGLTYLDNEPLGRVTAVPLY
                     RERYRLLVAADAPLGNRDTVSWAEVAKVPLCLLTPDMQNRRIIERLLRDTGVEPNITL
                     ESDSMILLYSHVRTGRWASVMPARLAATLGLTDVLRSIPIVGSADSAHTIGLVAPARE
                     PMTPLTAALVAEARRVAASLDD"
BASE COUNT          326 a          478 c          515 g          328 t
ORIGIN      
        1 cccgccggcc cagcaaaggc ccgacgtaaa catcgcacgc cgtggcccgc cttcctcgac
       61 ccagcgtccg cagaccgcgg ccgatgcacc caggaaaaag atcgcgatcg agaagatcca
      121 gccgagatct gtgagcttcc agtcgtccgg tgttgacgcc gtgatgccga gcaacttggt
      181 cattggtaga ttgaacacgc tgaaggcgta tgcttggccg atacacaaat ggatacagag
      241 tgcagccggc ggcaccagcc atcggctgaa tcctggattt gcaatcgaat gactgcggtc
      301 gagaaagctc attgcgcggt cccccttggt ttccctggac gccatttttc ttacagaccg
      361 cggacaggct ttgttgcccc ttcgagtctg ctgattgcac agtgcggatg gacatttgct
      421 gaatcaataa aagagttcca atcgcgcgat agaaatttct aatctcgcaa tagcgaacaa
      481 ctatgctgaa tccggacggc gatgcagcga gattttttgg aaattcctat ctcgatcgcg
      541 tctacgacta aaggcttagc gttcgacgcg gccatctgcg atcaatcgtc gagcgacgcc
      601 gccacgcggc gtgcttccgc gaccagcgcc gcggtcaatg gagtcatcgg ttcgcgcgcc
      661 ggcgccacca ggccgatggt atgcgccgaa tccgctgagc cgacgatcgg tatcgagcgc
      721 aacacgtcag tcaggccgag cgtggcggcg agccttgccg gcatcacgct ggcccagcgg
      781 ccggtgcgca cgtgcgagta cagcagaatc atcgaatccg attcgagcgt gatgttcggc
      841 tcgacgccgg tgtcgcgtag cagccgttcg atgatgcggc ggttctgcat atccggcgtg
      901 agcaggcaca gcggcacttt ggccacctcg gcccaggaga cggtgtcgcg gttgccgagt
      961 ggcgcatcgg cggcaaccag cagccggtaa cgctcgcggt agagcggcac ggcggtgacg
     1021 cggccgagcg gctcgttgtc gagataggta aggccggcgt cgatctcgag attttccaga
     1081 agcgctagga tctcgatcga ggtgcgcgag tagacggtga actgaacgtc cgggtggcgg
     1141 gcgcgatagg gcgtggtgag cgtctccacc atcgccagtg cggtcgggat cgcggcgagc
     1201 cgcaggcggc cgaccaggcc gtgcttgagc gcatgaactt cctcgcgcat ggcacgggca
     1261 tcgccgacga tgcggcgcgc ccaatcgagt acgcgttcgc cttccggcgt gaagccctgg
     1321 aagcgcgagc cccggttgac caggagcacg ccgaacgtgt cctcgatttg ctttacacct
     1381 gccgacaaag tcggctgggt gacgccacag gcctcggcgg cgtgcccgaa gtgcttttcc
     1441 cgcgccaatg ccagtagaaa ttcaagtttg tcgatcactg gagttctctt gctaagaccg
     1501 tgctggactt cgatatttga cgggcgaaac ggtgtatctc tgaactgccc ttgtggtggc
     1561 cgtaatcgga ttttacaaga atggataccg ttttgataga gaaaatctat catgtcattg
     1621 agggttaggc cttgttcgcc ttgattc
//
LOCUS       DASWZT010000539        19868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_52735, whole genome shotgun sequence.
ACCESSION   DASWZT010000539 DASWZT010000000
VERSION     DASWZT010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 19868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19868
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52735"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VGE92_15260"
     CDS             complement(<1..337)
                     /locus_tag="VGE92_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315238.1"
                     /translation="MSSIRISRRALALHIAAAPFALGAAAAGDGAAPGSDGLSHSAAA
                     IRQQLKFNAPRRRVYEALTNAGEFDAVTRLSDALALVTAPGAKPTSIRREAGGAFTLF
                     GGYITGRNLE"
     gene            432..728
                     /locus_tag="VGE92_15265"
     CDS             432..728
                     /locus_tag="VGE92_15265"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY1315239.1"
                     /translation="MFHALGDRTRRALLARLAKEPSMITELAKPFAMSLPAVSRHIRV
                     LESAGLVTRAVDGRVHQCSLCTKPFETIDAWLAHYRQFWESSLEALANYTETGD"
     gene            732..1160
                     /locus_tag="VGE92_15270"
     CDS             732..1160
                     /locus_tag="VGE92_15270"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY1315240.1"
                     /translation="MTALVLVVRRTIRASRERLFDAWTQPGQLKRWWGPKSVVCSDAQ
                     VDLKVGGSYRIANRFPDGNVVSISGEFEAVERPHLLVYTWRVGSEAGAAERVSVTFET
                     RGDATEVIVTHERIASAAIRDMHEQGWVGCLDGLAQYLAA"
     gene            complement(1157..1633)
                     /locus_tag="VGE92_15275"
     CDS             complement(1157..1633)
                     /locus_tag="VGE92_15275"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY1315241.1"
                     /translation="MAWAATPQIGAPAPAFHLQDQAGAWHDLSEYRGKWLVLYFYPKD
                     DTPGCTTQACEFRDNIFAFNKLGAVILGISVDDVASHKAFAEEHGLPFALLADDTKET
                     AKSYGVLRNALGLMEIASRETFIIDPQGRVAKHYAHVDPKGHSQLVLADLATLRAH"
     gene            1708..2907
                     /locus_tag="VGE92_15280"
     CDS             1708..2907
                     /locus_tag="VGE92_15280"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY1315242.1"
                     /translation="MNTPSNAGPESATGQWHTNIVPVETPQLGLVLTGGGARSAYQVG
                     VLKGVAELLRRGSACPFPIITGTSAGAVSAIALASDAAHFRRSVYAIERVWRDFRVHQ
                     VFKADAVSMLRSGLHWMLAFITGGWLVHSPHALFDNAPLWSLLREKLHFDGIPRSLYK
                     KHLQGIGVCATCYADADSVTFYASSSAIEPWARVFRKGARVSLTLDHLMASLAIPFLF
                     RPVKLHDQYFGDGAMRQTSPLSPAIHLGANRLFVIGVNDPVSAGAAQSRAVEPSFGQM
                     FGFMLDSLFMDQLNADLERISRYNELNDTRRIDFIVMTPSRDVNEIARRHARELPRSL
                     RALMRTMGASTPASTMLLSYLLFERGFTRELIELGYEDARARAQEIRGFLSLENARKF
                     RATSPDR"
     gene            complement(2916..3779)
                     /gene="rapZ"
                     /locus_tag="VGE92_15285"
     CDS             complement(2916..3779)
                     /gene="rapZ"
                     /locus_tag="VGE92_15285"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEY1315243.1"
                     /translation="MRLIIVSGLSGSGKSVALHMLEDIDFYCVDNIPAALLKPFISHT
                     VRGMGDTYPRTAVGLDARNRPNEIETIPALVTELRRSGIDCEVLYLHAADEVLLKRYA
                     ETRRKHPLVAGDVSLREAIANERRLLEPITIAADLVIDTSHMSVHALRELIRERIDRR
                     SEGRLSLMFESFGYKHGIPGDADFVFDVRSLPNPFWEHALRNLNGRDAAVVEYLSGYA
                     SVRNMIADLIAFLDRRVADFSEANRSYLTIAVGCTGGQHRSVYIAEKLAEHYRQSYPQ
                     VLTRHDSLHKA"
     gene            complement(3800..4123)
                     /gene="raiA"
                     /locus_tag="VGE92_15290"
     CDS             complement(3800..4123)
                     /gene="raiA"
                     /locus_tag="VGE92_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEY1315244.1"
                     /translation="MQLDLSGHHVEVTDSLRAYVVKKFERISRHFDQLIDVHCVLTVE
                     KFGHKAEATLMLRGNKIYADATEANMYASIDALADKLDRCVKKHKEKAADHHAEDVLK
                     ARLSG"
     gene            complement(4139..5611)
                     /locus_tag="VGE92_15295"
     CDS             complement(4139..5611)
                     /locus_tag="VGE92_15295"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEY1315245.1"
                     /translation="MLKPSLQLRIGQQLTMTPQLQQAIRLLQLPSLDLQAHVRETLET
                     NVMLEADEENVDTIGEIAREREDAPEEKDEKYAELDGNTTPTEQREEPEVEIADEAWG
                     EQTSGPSDTPWSGDDDRSTDFSDQHGQTLQEQLIEQLELAKLSAVDMAIARVITDAIT
                     DDGYLKDELEDIRLSLLPEIEATTADVERVLGAVQSLEPAGVGARNLGECIALQLRQL
                     HPDTPARDTAIRVALEHLDLVAGQQLVLLRRQLRCSEADLEMALALVRSCHPRPGAAV
                     NPAQAEYVIPDVFVRRTEHGWTVEINQATVPRVRVNNAYANLISRSPDHAMLRTQLQE
                     ARWLMRSLEIRNETLIKVARCIVQRQTSFFELGEEAMEPLILKDVAETVEMHESTISR
                     VTTAKYMHTPRGVFEFRYFFSSHVEAADGTEMSSTAIRAKIKKLIAQENPENPWSDSK
                     LAEILSQEGIPVARRTVAKYREGMQIAPSNERKRAAARAS"
     gene            complement(5631..6356)
                     /gene="lptB"
                     /locus_tag="VGE92_15300"
     CDS             complement(5631..6356)
                     /gene="lptB"
                     /locus_tag="VGE92_15300"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005096670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEY1315246.1"
                     /translation="MSVLRALNLAKSYKSRQVLRGLSLEVESGEVVGLLGPNGAGKTT
                     AFYMIVGLARCEQGEIYLDNRDISRLPMHRRAQLGLGYLPQEPSVFRRLSVADNIMAI
                     LETRPGLDDEGRTARLKELLGELHIGHVRDSLGMSLSGGERRRVEIARALAADPRFIL
                     LDEPFAGVDPLSVADIQRIIKHLSGKGIGVLITDHNVRETLGICARAYIVSQGTVLAS
                     GSADQILANPQVREVYLGQDFKL"
     gene            complement(6353..6955)
                     /gene="lptA"
                     /locus_tag="VGE92_15305"
     CDS             complement(6353..6955)
                     /gene="lptA"
                     /locus_tag="VGE92_15305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEY1315247.1"
                     /translation="MAVSTLKDFLALLVVALPVSQATAAQKNEQPLVIEAQSVDLDYK
                     NNNVLYHKVRIAQGTMSVAADQAQTTGVDVDNSHWVFRGGVKISMNQGQLTADEAEIT
                     FANKLLSKALVNGKPAKFEQQIAKTGKLAQGRADTIDYDVKQGVVHLSKNAWLSDGQH
                     EILGESLKYNVLSQNIVAESSEQGSQRVHIIITPPANPKP"
     gene            complement(6936..7493)
                     /gene="lptC"
                     /locus_tag="VGE92_15310"
     CDS             complement(6936..7493)
                     /gene="lptC"
                     /locus_tag="VGE92_15310"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEY1315248.1"
                     /translation="MLFRFFTVLAVVALGISTWILSSPERRPHALIAASQAELPGYYL
                     KNAVLTDYDLAGHPSVRIEAERIDQIDHGNEVALYNVRVVYQAPGGQSWIMLGDTAHV
                     QPGGKAIDVKGNVRLQGEATDRAGAAVIHTDTLTYDVPDSIATTKDDVRIDFGQHTLT
                     AHGLSANFKERTLRLESKVNGRFHP"
     gene            complement(7493..7996)
                     /locus_tag="VGE92_15315"
     CDS             complement(7493..7996)
                     /locus_tag="VGE92_15315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEY1315249.1"
                     /translation="MKARAATPLAKIQLLVLDIDGVLTDGRLYFGARGEALKVFHVRD
                     GHGIKLLMAAGVAVAAVSGRRSAAVAARMRELGVPHVIQACSDKVAALRRLTRHLGIS
                     ALNCACICDDTADLPLMSAVGMAGAVADAHPVVLSAAHWIAAANGGAGAVRELCDAVL
                     RARAGRR"
     gene            complement(7993..8976)
                     /locus_tag="VGE92_15320"
     CDS             complement(7993..8976)
                     /locus_tag="VGE92_15320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEY1315250.1"
                     /translation="MNLEFTDEALVDLGRQALTVEIDGLRAQLPRLGPEFARACRICL
                     SCRGRIVVTGMGKSGHVAGKIAATLASTGTPAFFMHPGEASHGDVGMITRDDAVLVLS
                     NSGETDEILTIVPVIVRLGVPLIAFTGNSNSTVARVATVHLDIGVPAEACPLNLTPTA
                     STTAALAVGDALAVALLKARGFTEDDFARSHPSGSLGRRLLLHVQDVMRTGADIPTVS
                     AGTPLAEGLLEVTRKGLGMTAIVDASRRVLGVFTDGDLRRALDAAVDLHATRMEQVMT
                     RHAKTVRPTTLAAEAVHLMETHRITALVVVDAEQRVVGALNVHDLLRAGVV"
     gene            9052..9297
                     /locus_tag="VGE92_15325"
     CDS             9052..9297
                     /locus_tag="VGE92_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HEY1315251.1"
                     /translation="MNSQQVAQIIESGLAGSRALVKSDDNVHFEAVVIAAAFKGKRSV
                     QRHQLVYGTLGAAVGNEIHALALQVFTPEEFAGQGRG"
     gene            9290..10549
                     /gene="murA"
                     /locus_tag="VGE92_15330"
     CDS             9290..10549
                     /gene="murA"
                     /locus_tag="VGE92_15330"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY1315252.1"
                     /translation="MDKLQIQGGIPLEGEIKISGAKNATLPILAGCLLADGPVTVSNV
                     PHLQDVTTMIELLGRMGVSVTIDEKMRIEVDASTIKEYFAPYQLVKTMRASILVLGPL
                     LARFGRADVSLPGGCAIGARPVNIHVAGLQAMGADIHIENGYIRARAGRLRGARLVLE
                     TVTVTGTENLMMAATLADGETVLENAAREPEVVDLANFLTAMGAKIRGAGTDKIVIEG
                     VTRLNGTAYEVLPDRIETGTYLVASAITGGQIRVKNTRPEHLDAVIGKLREAGAKVGT
                     GDTWIEVDMRGRRPAAVDIRTAPYPAFPTDMQAQFAALNTVASGVGTIIETIFENRFM
                     HMLEMRRMGAEIRLEGNTAIIKGVDKLTAAPVMATDLRASASLVLAGLVADGRTEIER
                     IYHIDRGYEAIEEKLQQLGAKIRRTPA"
     gene            complement(10559..11704)
                     /locus_tag="VGE92_15335"
     CDS             complement(10559..11704)
                     /locus_tag="VGE92_15335"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY1315253.1"
                     /translation="MQHARVFASFLIRAVIVGLAAAFLVVWWKPALLGKTTLPAQSTP
                     PAAGRPIVVMQSFADAVARASPAVVNIYTERVVTERTQSPALSQLFGDYWPSYRQRVE
                     RSLGSGVIVDAAGTIVTNQHVIAGADSIKVQLADARIAGASIVGQDPDTDIAILHLDI
                     GKLPIMPVGRSDTLRVGDIVLAIGNPYGLSQTVTQGIVSATGRGQLGLATFENFIQTD
                     AAINLGNSGGALIDAQGDLVGINTAVLNRAYGGPEGIGFAIPVNLVRGVMEQILAHGH
                     VVRGWLGFVPQDLTEEQAAQLGIPSGGGVTIVNILRTSPAYQAGIRPGDLVTALAHEP
                     VRNSQDLVSRVAALPPGSEVEIEGRRERQSYKLALKVLERPTSPAQP"
     gene            11830..12450
                     /gene="petA"
                     /locus_tag="VGE92_15340"
     CDS             11830..12450
                     /gene="petA"
                     /locus_tag="VGE92_15340"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEY1315254.1"
                     /translation="MSEDVDHSRRRLLTAATVGTGAIGVAFAAVPFLASWQPSARAKA
                     LGAPVEVDASKLEAGQMLKVVWRGQAVFVVRRVKGIIDQLGGHDALLADPQSDDSIQP
                     EYIKATGAARARNPEYWVGLAVCTHLGCSPLGAFEANNSFQVAGADLGANWPGGFYCP
                     CHGSKYDLSGRVFKSMPAPKNLTVPPYVFADGARITIGVDDAPKKA"
     gene            12469..13734
                     /locus_tag="VGE92_15345"
     CDS             12469..13734
                     /locus_tag="VGE92_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014592292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEY1315255.1"
                     /translation="MAAAPESADSSKGGSLIGWIDQRFPLTKLWKEQVAEYYAPKNFN
                     FWYYFGVLSIVVLVNQLITGIFLTMNYKPSAAEAFSSVEYIMRDVDWGWLIRYMHSTG
                     ASAFFIVIYLHMFRSLLYGSHRKPRELIWIFGCLIFVLLMAEGFMGYVLPWGNMSYWG
                     AQVIVSLFGSVPYVGDTLVEWIRGDYYIADATLNRFFALHVAALPLALVFLVIAHLMA
                     LHEVGSNNPDGIEIKKYKDANGHPLDGIPFHPYYTVKDTVGVAGFLLLFCGVIFFAPT
                     FGGLFLESPNFEPANALQTPPHIAPVWYFTPFYAMLRAVPSFAGTQVWGVLVMGASIV
                     VLFFLPWLDRSPVRSIRYRGPIYKIMLTIFVLSFVCLGLCGLKPPSGIYPTLAKICTV
                     LYFAFFLLMPWYTKFESVKPVPERVTVHG"
     gene            13727..14467
                     /locus_tag="VGE92_15350"
     CDS             13727..14467
                     /locus_tag="VGE92_15350"
                     /inference="COORDINATES: protein motif:HMM:NF014247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEY1315256.1"
                     /translation="MVKSWVFIAIALISAGNALAEEGDMALLPSGTDIKSMESLQRGA
                     RNFMNYCSGCHSLKYQRYNRLAADLKIPESELTADLMFTSDKAVDTINSAMPKDAEAW
                     FGKQPPDLSLMARERGTDYLYSFLKGFYADKTRPWGVNNLYLPGAAMPHVLQTLQGLQ
                     KPVFKNEPDERGSARMVLVGVDAMTPGALKPEEYDQFVRDIANFLDYVGEPVKEKRQS
                     MGVFVTLFLLVFFVFAYLLKKEYWKDVH"
     gene            14515..15132
                     /locus_tag="VGE92_15355"
     CDS             14515..15132
                     /locus_tag="VGE92_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017138266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY1315257.1"
                     /translation="MARRSVMTLFSAPADPWSHRTRLVLAEKGIAIELVNVDVNDLPE
                     DLLDLNPYHSVPTLVDRDLVLYDSRVIIEYLDERFPHPPLMPIDPVTRAQFRVALYRV
                     ERDWYALARDIELDPQSKTAAHSRKVLGEAICASADVFKAKPFFLSDEFSLVDASIAP
                     ILWRLPSYGIELPVQAQPITKYMNSIFSRSTFRSCLTEVERGMRH"
     gene            15129..15536
                     /locus_tag="VGE92_15360"
     CDS             15129..15536
                     /locus_tag="VGE92_15360"
                     /inference="COORDINATES: protein motif:HMM:NF016286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEY1315258.1"
                     /translation="MKPRRPYLLRALHEWISDSGETPHIVVDAAAEGVSVPRQYVKDG
                     KIVLNVSLSATQMLKLGNDVVSFEARFGGVSFAVQVPVRAILGIYARETGQGMIFPEG
                     DADPDPSDVPPPGPAAGKDSGSKAKRPKLQVVK"
     gene            complement(15529..15900)
                     /locus_tag="VGE92_15365"
     CDS             complement(15529..15900)
                     /locus_tag="VGE92_15365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEY1315259.1"
                     /translation="MSDQAWKRGAVAEQLAAAYLESQGLRILARNLRCKLGELDLVCL
                     DRGVLAIVEVRQRMRIDYGGALASVDHRKQRKLMRAARYFLLRRAAWRGLMMRFDVVG
                     VQGPAEGPQHIVWIKDAFRSI"
     gene            15973..16983
                     /locus_tag="VGE92_15370"
     CDS             15973..16983
                     /locus_tag="VGE92_15370"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY1315260.1"
                     /translation="MPEYRNNYIGSVTKSLGELLRWQFERVRSRLPPAPTVPIPTVAA
                     DLDFVQGNAVAAGAMIPAVTWIGHASVLVQACGLNVLTDPIFSSRASPVQFAGPARLQ
                     APGIALRDLPHIDVVVISHNHYDHLDRGSIQTLARQAGGPPLFLVPLGIKAWLKRAGI
                     ERAVELNWGQMQVHEQVEFHFTPAQHWSGRCLSDRNRTLWGSWAVLGAQLHWFFSGDT
                     GYSKDFRDIRERFAPRQTTAQGGAFDIALIAVGACLPRWFMKEQHVDLDEAMQIHLDL
                     GAKRSIGVHWGTFSLADDPPDLPLHELAHTRRGKGVADEEFFLLPVGGTRRLARRLEA
                     HR"
     gene            16980..17507
                     /locus_tag="VGE92_15375"
     CDS             16980..17507
                     /locus_tag="VGE92_15375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315261.1"
                     /translation="MTNSLLAGWDNFYVITGSSAAGLTGLTFVVIALAAEAKRVSLKG
                     LRTYVTPTIVHFGAVLALAAYLSMPNQGVLSLSLGFGAAACAGLAYVGSIAASIGRIS
                     KDYMPVAEDWIWNVILPTIVYGTLLGMAVLIWTRPEVSMYGVAAVLVLLMFIGIHNAW
                     DIAVWNSVRKLDDSA"
     gene            17599..17811
                     /locus_tag="VGE92_15380"
     CDS             17599..17811
                     /locus_tag="VGE92_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315262.1"
                     /translation="MNSAVDNVLHQLQLGEDLIADGIEVLARHWQASTTAIGKLCTGS
                     DRWQAALREIQTQVNLAEIFRGRTII"
     gene            complement(17808..19526)
                     /locus_tag="VGE92_15385"
     CDS             complement(17808..19526)
                     /locus_tag="VGE92_15385"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY1315263.1"
                     /translation="MIAVDIARCGALAVLSGALAVAHAASPDKPAADLIIRHARIWTL
                     NPAQAEAQAVAVLNGRIVAVGSDGAVLTWAGSRTQVVDAEGKRLLPGFNDAHVHFEDG
                     GASLSAVQLNDAPSLEVFVKRIGDYAARAAKGEWILAGDWDETKWSPAKLPTRQDIDA
                     VTPNNPLAIDRYDGHMILVNSKALALAGITAATPNPPGGVIVRDAAGQPTGALADAAA
                     GLVERVIPEPTAAQRRRAIEGALREAARRGVTSVQDMREDYANLAVYSELLRDGKLTV
                     RLYAAPPVKAVDDQARLGIGRAFGGPFLRIGALKMFADGSLGSRTAYFFEPFLDTPDI
                     RGLLAEDMLPPGRTGQRLLRADAAGLQVCTHAIGDAAISTVLDLYDDVATADGPRDRR
                     WRIEHAQHMAARDFDRFAQLHVIASVQPYQAIDDGRWAEARIGHERASRTYAFRTFLD
                     HGVRLAFGTDWPVAPLDPMLTLYAATTRATLDGKHPEGWFAEQKLSIQEAIFAYTLGS
                     AYAEFQEHDKGSIEVGKLADLVLLSEDVLAIPAARIREVHVLKTWVGGEPVFDSSATP
                     VRAAAN"
     gene            complement(19523..>19868)
                     /locus_tag="VGE92_15390"
     CDS             complement(19523..>19868)
                     /locus_tag="VGE92_15390"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEY1315264.1"
                     /translation="VYSTSDSFEPDSAANSDLDGMLFPDMPWMVADDPVTTQIRDGVR
                     AAWPARTARRDRLYAFGFDAYRLVPALRSKSPAEASEISGVTGKLHLDDRNRIHRELQ
                     WAQIKNGEPAGL"
BASE COUNT         3592 a         6118 c         6242 g         3916 t
ORIGIN      
        1 actccaggtt ccggcccgtg atatagccgc cgaatagcgt gaaggcaccg ccagcctcgc
       61 gcctgatcga cgtgggtttg gcgcccggcg ccgtcaccag cgccagcgca tcgctcaaac
      121 gagtgacggc gtcgaactcg ccggcattgg tcagcgcttc gtatacgcgc cgacgcggtg
      181 cgttgaattt aagttgttgg cgaattgccg ccgcggagtg agacagcccg tcgctgcccg
      241 gagcggcgcc gtcgccggcc gccgcggctc ccaacgcaaa cggcgccgcg gcaatatgca
      301 gcgccaatgc gcggcgcgag atgcgaatcg aggacacagg cagacggcct ctttgcattg
      361 tttaacaagt ttgtttaata tagttttaaa ggagccgccg cacattgtca agagaagagc
      421 atctcgatgc agtgttccat gccctgggcg accgaacccg gcgcgcactg ctggcacgcc
      481 tggctaaaga gccgtcgatg atcacagaac ttgcgaaacc cttcgccatg tcgctgccgg
      541 cagtatcgcg gcacatccgt gtactcgaga gcgcggggct ggtgacccgc gccgtcgatg
      601 gccgcgtgca ccaatgctcg ctctgcacca agcccttcga gaccatcgac gcttggctcg
      661 cgcactaccg gcaattctgg gagagcagtc tcgaggcatt ggccaactac accgagacgg
      721 gcgattagcg cgtgacggcg ctggtactcg tcgttcgccg tacgattcgc gcaagccgcg
      781 agcgcctgtt cgatgcgtgg acccagcccg ggcagctcaa acgatggtgg ggtccgaaat
      841 cggtggtgtg cagtgacgcg caggtcgatt tgaaagtggg tggcagttat cgcatcgcca
      901 atcggtttcc ggacggcaat gttgtatcga tcagcggcga attcgaagcc gtcgaacgcc
      961 cgcacctatt ggtttacacc tggcgggtgg ggtccgaggc cggcgcggca gagcgagtga
     1021 gcgtgacatt cgagacgcgc ggcgacgcca ccgaggtcat cgtcacgcat gaacgcattg
     1081 ccagcgccgc aatacgcgac atgcacgagc agggttgggt gggctgcctt gacggccttg
     1141 cgcaatacct cgccgcctaa tgagctcgca gcgttgctaa atccgccagc accaattgcg
     1201 agtgtccttt cggatcgaca tgcgcgtagt gcttcgcaac ccggccctgc ggatcgatga
     1261 tgaatgtctc gcggctggcg atttccatca agccgagcgc gttgcgcagg acgccgtagg
     1321 atttggcggt ttccttggta tcgtccgcca ataaggcgaa cggcagacca tgctcttcgg
     1381 cgaacgcctt atgggaggcc acgtcgtcca cgctgatgcc gagaatcacc gcgcccaact
     1441 tattgaaggc gaagatgtta tcgcgaaatt cgcaggcctg ggtcgtgcag cccggcgtat
     1501 cgtccttcgg atagaagtac aacaccagcc atttgccgcg gtattcgctt aagtcatgcc
     1561 aggcgccggc ctggtcttgc aagtggaaag cgggcgcggg cgcgccgatt tgcggagtcg
     1621 cggcccacgc cacagtcgcg cctaggcagg cgcagtacaa tgcgcccagc agcccaattc
     1681 tcgttccagt gctatggtgc ttgaccattg aatactccga gcaatgcagg tcccgaaagc
     1741 gccaccgggc aatggcatac taacatcgtg ccggtcgaga caccccaatt ggggctggtt
     1801 ctgacggggg gcggcgcgcg ctccgcttat caggtcgggg tactcaaggg cgtcgcggaa
     1861 ctgttgcggc gcggctcggc gtgtccgttt ccgatcatca ccggaacctc cgccggcgcg
     1921 gtcagcgcca tcgctctcgc ctcggacgcc gcacacttca ggcgctcggt ctatgcgatc
     1981 gagcgggtat ggcgcgattt tcgcgtgcat caggtattca aagccgatgc ggtgagcatg
     2041 ctgcgctccg gcttgcactg gatgctggca ttcatcaccg gcggctggct ggtgcattcg
     2101 ccgcacgcgc tgttcgacaa tgcgccgctg tggagcttgc tgcgcgagaa attgcatttc
     2161 gacggcattc cccgcagcct ctataaaaag cacctgcagg ggatcggcgt gtgcgccacc
     2221 tgctatgcgg atgcggattc ggtcactttc tatgcgtctt cgtccgcgat cgaaccgtgg
     2281 gcgcgggtat ttcgcaaggg cgcgcgggta tcgctgacgc tcgatcacct gatggcaagt
     2341 ctggccatcc cgtttctgtt ccggccggtg aagctgcatg accaatactt cggcgacggc
     2401 gccatgcgcc aaacctcgcc cctgagtccc gcgatccatc tcggcgcgaa ccggttgttt
     2461 gtcatcggcg tcaacgatcc ggtctcggcg ggcgcggccc agagccgcgc ggtggagccg
     2521 agctttgggc aaatgttcgg cttcatgctg gattctttgt tcatggatca gctgaatgcg
     2581 gatctcgagc gcatcagccg ctacaacgaa ctcaacgaca cgcggcgcat cgatttcatc
     2641 gtcatgaccc cgtcgcggga cgtcaacgaa atcgcgcgcc gccacgcccg agaattgccg
     2701 cgcagcctgc gtgcgctgat gcgcacgatg ggtgcgagca cccccgcaag caccatgctg
     2761 ctgagttatc tgttgttcga acgcggtttc acacgggaat tgatcgaact cggctacgag
     2821 gacgcgcgcg cgcgcgccca ggagattcgc ggcttcctgt cactggagaa tgcccgaaaa
     2881 ttccgtgcaa cctcgcccga tcgctgacgc cgcgatcagg ctttgtgcag ggaatcgtgg
     2941 cgcgtgagga cttgcgggta ggactgacgg tagtgttccg cgagcttctc cgcgatgtac
     3001 accgagcggt gctggccgcc ggtgcagccg acggcgatgg tgagatagct gcggttcgcc
     3061 tcggaaaaat cggcgacccg ccggtccagg aatgcgatca gatccgcgat catattgcgc
     3121 acgctcgcgt agcctgacag atactcgact acggcggcgt cgcggccgtt gaggttgcgc
     3181 agcgcatgct cccaaaacgg attcggcagg ctgcgcacgt cgaacacgaa atccgcatcg
     3241 cctggtatgc cgtgcttgta gccgaatgac tcgaacatca aggacaagcg cccttcgctg
     3301 cgccggtcga tccgttcgcg aatcagctcg cgcagcgcat gcacgctcat gtgcgaagtg
     3361 tcgatcacca aatccgcggc aatggtgatc ggctccagaa gccgacgttc gttggcgatc
     3421 gcctcgcgca gactgacgtc gccagcgacc aacggatgct tgcggcgcgt ctcggcgtag
     3481 cgcttgagca gtacctcgtc ggcggcgtgc aggtatagaa cttcgcagtc gatgccgctg
     3541 cgccgcagct cagtcacgag agccgggatg gtctcgatct cgttgggccg gttgcgcgcg
     3601 tccaatccga ccgcggtcct gggataggtg tctcccatgc cgcggacggt gtgcgagatg
     3661 aagggtttga ggagggctgc gggaatattg tcgacacaat agaaatcgat gtcctccagc
     3721 atgtgcagag cgacgctctt gcccgagcct gagagcccgc tcacgatgat gagtcgcatc
     3781 gacgcgcggc cctttgagac tatcccgaca agcgcgcctt gagcacgtct tcggcgtgat
     3841 ggtccgcagc cttttccttg tgcttcttga cacatcgatc gagcttgtcg gcgagcgcat
     3901 cgatggaggc gtacatattc gcttcagtgg catccgcgta gatcttgttt ccgcgcagca
     3961 tcagcgtggc ttccgccttg tgtccgaatt tttccaccgt gagcacgcag tggacatcga
     4021 tcagttggtc gaaatgccgg gaaattcgct cgaatttctt cacaacatac gcgcgtaatg
     4081 agtcagtaac ttctacatgg tgaccgctga gatcgagctg catgtttcct ccttcgattt
     4141 atgaggcgcg ggcagccgct cgcttccgtt cgttggacgg tgcgatctgc atgccttcgc
     4201 ggtacttggc aacggtgcgc cgcgccaccg gtatgccctc ctgcgacaat atttccgcga
     4261 gcttgctgtc gctccacgga ttttcaggat tctcttgtgc aatcagtttc ttgatcttgg
     4321 cgcgaatggc ggtggaggac atttccgtgc catcggcggc ctcgacatga ctcgaaaaga
     4381 agtatctgaa ctcgaacacg ccgcgcggcg tgtgcatgta ttttgcggtg gtcacccgcg
     4441 aaatggtcga ttcgtgcatt tccacggttt cggccacatc cttgaggatc agcggttcca
     4501 tcgcttcctc gcccaattcg aagaagctcg tctggcgctg cacgatgcag cgcgcgacct
     4561 tgatcaaggt ttcgttgcgg atttccaggc tgcgcatcag ccagcgcgct tcctgcagtt
     4621 gggtgcgcaa catggcgtgg tcgggggagc gcgagatcaa attcgcgtag gcattgttga
     4681 cgcgcacccg cggtaccgtt gcctggttga tctcgaccgt ccagccgtgc tcggtacggc
     4741 gcacgaaaac atccgggatg acatattctg cctgcgccgg gttcaccgcc gcgccggggc
     4801 gcgggtggca ggagcgcacc agcgccagcg ccatttccaa gtcagcttcg ctgcagcgta
     4861 gctggcggcg caacagcacc agctgctggc cggcgaccag gtccaagtgt tccagcgcca
     4921 cgcggatcgc ggtatcgcgg gccggggtgt ccggatgcag ctggcgcagc tgcagggcga
     4981 tgcactcgcc gagattgcgg gcaccgacgc ccgccggttc caacgattgc acggcgccga
     5041 gcacccgctc cacgtccgcc gtcgtagcct cgatttccgg cagcaagctc aagcggatgt
     5101 cttcgagttc gtccttaaga taaccgtcgt cggtaatggc gtcggtaatg acgcgcgcta
     5161 tagccatatc gacggccgac agcttggcga gttccagctg ctcgatcagt tgctcctgca
     5221 aagtttggcc atgctggtcg ctgaagtccg tgctgcggtc atcgtcgccc gaccacgggg
     5281 tgtccgacgg gccgctggtc tgttcgcccc aggcctcatc cgcaatttca acctcgggct
     5341 cttcgcgctg ttcggtggga gtggtgttgc cgtcgagctc ggcgtatttc tcgtcctttt
     5401 cctcgggcgc gtcctcgcgc tcgcgcgcga tctcgccgat ggtatcgacg ttttcctcgt
     5461 cggcctcgag catgacattg gtctcgagag tctcgcgcac atgcgcctgc agatcgaggg
     5521 acggcagctg cagcaagcgg atcgcctgct gcaattgcgg cgtcatcgtg agctgctggc
     5581 cgatgcgaag ctgcagactt ggcttcaaca tagttctttc aaatccaagc ctacagtttg
     5641 aaatcctggc ccaagtacac ctcacggacc tgcgggttgg caagaatctg atccgcagat
     5701 ccggaggcga gcaccgtacc ctggctgaca atataagcgc gcgcacagat gccgagagtc
     5761 tcccttacat tatggtcggt aattagcaca ccgatgccct taccggacaa gtgcttgatt
     5821 attcgctgga tatcggcaac cgacaggggg tcgacgccgg caaatggctc atccagcaag
     5881 atgaacctcg gatccgccgc caaggcgcgg gctatctcca cccggcggcg ctcgccgccc
     5941 gacaggctca ttccaaggct gtcgcgcaca tggccgatat gcagttcgcc caggagctcc
     6001 ttgaggcgcg cggttcgccc ctcatcgtcg agcccgggcc gcgtttccag tatggccatt
     6061 atgttgtccg ccacggacag ccgccggaaa accgagggct cctgcggcag gtagccgagc
     6121 ccgagctgcg cccgtcggtg catcggcagg cggctgatat cgcgattgtc cagatagatt
     6181 tcgccctgtt cgcagcgggc gaggccgaca atcatgtaaa acgccgtggt cttgccggcg
     6241 ccattcgggc cgagcaggcc gaccacctcg ccgctttcga cttccaacga caaaccccgc
     6301 agaacctggc gcgatttgta acttttggcg aggttcaggg cccgcaagac actcatggtt
     6361 tgggattggc cggcggcgtg atgatgatgt gcacgcgctg ggacccctgt tccgaacttt
     6421 ccgcgacgat gttctgcgac agcacgttgt atttcagcga ctcgccgaga atctcgtgct
     6481 ggccgtcgct caaccacgca ttcttcgata gatgtaccac gccctgcttg acgtcgtaat
     6541 cgatggtgtc ggcgcgccct tgagcgagtt tccccgtctt cgcgatctgt tgttcgaact
     6601 tggccggctt gccgttgacc agcgccttcg acagcagctt gttggcgaag gtgatctcgg
     6661 cctcatccgc cgtgagctga ccctggttca tgctgatctt gacgccgccg cgaaacaccc
     6721 aatggctgtt gtcgacatcc acgcccgtgg tctgggcctg atcggcggcg accgacatgg
     6781 tgccttgcgc gatgcgtacc ttgtggtaca gcacattgtt gttcttgtag tcgagatcga
     6841 ccgactgtgc ctcgatgacc agcggctgct catttttttg cgcggccgtg gcctgcgaca
     6901 cgggcagggc gacgacgagc aatgcaagga aatccttaag ggtggaaacg gccattgacc
     6961 ttcgattcca ggcgcaacgt gcgctccttg aaattggcgc tgagcccgtg tgcagtcaga
     7021 gtatgttggc cgaaatcgat gcgcacgtca tccttggtgg tggcgatgga atcgggtacg
     7081 tcgtaggtca aggtgtcggt gtggatcacg gccgcgccgg cccggtccgt ggcctcgcct
     7141 tgcagccgca cgttgccctt cacatcgatg gccttgccgc ccggctgcac gtgcgcggtg
     7201 tcaccgagca tgatccacga ttggccgccc ggcgcctgat acaccacgcg cacgttgtac
     7261 agcgccacct cgttgccatg gtcgatttga tcgatgcgct cggcctcgat gcgcacgctc
     7321 ggatggccgg cgagatcata atcggtgagt acggcgttct ttaggtagta ccccggcaat
     7381 tcagcctgac tggcggcgat cagcgcatgc gggcggcgtt ccggagagct caagatccaa
     7441 gtgctgatgc cgagcgccac gacggcgagc acggtgaaga atcggaatag catcagcgcc
     7501 gcccggcgcg cgcgcgcagt acggcgtcgc acagttcgcg caccgcgccc gcgccgccgt
     7561 ttgccgccgc aatccagtgc gccgctgaga gcaccaccgg gtgcgcatcc gcgaccgcgc
     7621 cggccatgcc gacggcggac atcaggggca aatcggcggt gtcgtcgcag atgcaggcgc
     7681 agttgagcgc gctgatgccg agatgccggg tcaggcggcg cagcgccgcc actttgtcgc
     7741 tgcacgcctg aatcacatgc ggcacgccga gttcacgcat gcgcgctgcg accgcagccg
     7801 agcgccgtcc gctgacggcg gccacggcga ccccggccgc catgagaagt ttgatgccat
     7861 gcccatcgcg cacgtggaac actttgagcg cctcgccgcg cgccccgaaa tacaagcgcc
     7921 cgtcggtcag cacgccgtcg atgtccagaa ccaggagttg gattttcgcc agcggcgtcg
     7981 ccgcgcgcgc cttcaaacca cacccgcgcg cagcagatca tgcacgttga gggcgccgac
     8041 gactcgctgc tcggcatcga ccaccaccag ggccgtgatg cggtgggttt ccatcagatg
     8101 cacagcctcc gcggcgagcg tcgtcggtcg caccgttttt gcgtgacgcg tcatgacctg
     8161 ctccatgcgc gtcgcatgca aatccacggc cgcatcgagc gcgcggcgca gatctccgtc
     8221 ggtgaatacg ccgaggaccc ggcggctcgc atcgacgatg gccgtcatgc cgaggccctt
     8281 gcgagtcacc tcgagcagac cttcggcaag cggcgtaccg gcgctgaccg ttggaatatc
     8341 ggcgccggtg cgcatgacat cctgcacatg cagcaacaag cgccggccca gacttccgga
     8401 ggggtgcgag cgggcgaagt catcctcggt gaagccgcgc gccttgagga gggccacggc
     8461 cagcgcgtcg cccaccgcca acgccgcggt ggtgctcgcg gtcggggtca aattgagcgg
     8521 gcaggcctcg gccgggacgc cgatgtcgag gtgcacggtc gcgacacgcg cgaccgtgga
     8581 attggagttg cccgtaaagg cgatcagggg tacccctaga cgcacgatca ccggcacgat
     8641 ggtgaggatc tcgtcggttt cgcccgaatt cgacaagacc agcaccgcgt cgtcacgcgt
     8701 aatcatgccc acatcgccgt gactcgcttc acccgggtgc atgaagaacg cgggcgtgcc
     8761 ggtgctcgcc agggtcgcgg cgatcttgcc ggcgacatgc ccggacttgc ccatgccggt
     8821 gaccacgatg cgtccacggc agctgagaca aatcctgcag gcacgggcaa actcgggccc
     8881 caggcgcggc aattgcgcgc gcaggccgtc gatctcgacg gtgagcgcct gccggccgag
     8941 atcgacgagt gcttcgtcgg tgaactcgag attcattctc gaatgataca gcggttgcag
     9001 aatctcgcgt ggccgaaggc gtaagcttgg gattattgga gaggagcggg catgaattca
     9061 caacaagtgg cgcaaatcat cgagtcgggc ctcgcgggca gccgggcgct cgtcaagagc
     9121 gacgacaacg tgcatttcga ggccgtggtc atcgccgctg cgttcaaagg caagcgatcg
     9181 gtgcagcgcc atcaactggt gtacggcacg ttgggggcgg cggtcggcaa cgaaattcac
     9241 gcgctcgctc tgcaggtgtt cactccggaa gagtttgcgg gccagggacg tggataagtt
     9301 acagattcag ggcggcatcc cgctcgaagg cgaaatcaag atctccggcg cgaagaacgc
     9361 caccctgccg atattggccg gctgcctgtt ggcggacggt ccggtgacgg tgtcgaacgt
     9421 tccgcacctg caggacgtga ccacgatgat cgagctgctc ggacgcatgg gcgtctcggt
     9481 gaccatcgat gaaaagatgc gcatcgaggt cgatgcttcg accatcaagg aatatttcgc
     9541 gccgtatcag ctggtgaaaa ccatgcgcgc ttcgatcctg gtgttgggtc cgttgctggc
     9601 gcgtttcggc cgcgccgacg tgtcgttgcc cggcggctgc gcgatcggcg cacgtccggt
     9661 caacatccat gtcgcgggtc tgcaggcgat gggcgccgac atacacatcg agaacggcta
     9721 tatccgcgcg cgcgcggggc gtctgcgagg cgcccgcctg gtgttggaga ccgtcaccgt
     9781 gaccggtacc gagaatctca tgatggcggc gaccctggcc gatggtgaaa ccgtgctcga
     9841 gaacgccgcg cgcgagcccg aagtggtcga tctggctaat ttcttgaccg ccatgggcgc
     9901 caagatccgg ggtgcaggaa ccgacaaaat tgtcatcgag ggtgtcacgc gactcaacgg
     9961 cacggcctac gaagtgctgc ccgatcgtat cgagaccggc acctacctgg tcgcaagcgc
    10021 catcaccggc ggtcagatcc gcgtcaagaa tacgcgcccg gaacacttgg atgccgtgat
    10081 cggcaagctg cgcgaagcgg gcgccaaagt gggcaccggc gatacatgga tcgaagtcga
    10141 catgcgcggg cgacgccccg ctgcggtcga catccgcacc gcgccgtatc cggcttttcc
    10201 gaccgatatg caggcgcaat ttgccgcgct caacaccgtc gccagcggcg tcggcacgat
    10261 catcgaaacg attttcgaga atcgcttcat gcacatgttg gaaatgcggc gcatgggcgc
    10321 tgagatccgt ctggaaggca atacggcgat catcaagggc gtcgacaagc tcaccgccgc
    10381 gccggtcatg gcgaccgacc tacgcgcgtc ggcgagtctc gtgctggcgg gactggtcgc
    10441 cgacggccgc acggagatcg aacggatcta tcacatcgac cgcggctacg aagcgatcga
    10501 ggagaagctg cagcaactcg gggccaaaat ccgccgcacc ccggcttgac ttgtctccct
    10561 agggttgcgc cgggctcgtc ggccgctcca acaccttgag tgcgagtttg tacgactggc
    10621 gctcgcggcg gccttcgatt tcgacttctg agcctggcgg cagcgccgcc acccggctga
    10681 cgaggtcctg ggagttgcgg accggttcat gggccaaggc agtcacgagg tcccccggac
    10741 gaatgccggc ctggtacgcc ggactcgtgc gcagaatatt cacgatggtc accccgcctc
    10801 ccgaagggat tcccaactgc gccgcttgtt cttcggtcaa atcttgcggc acgaagccca
    10861 accagccgcg caccacatgt ccgtgcgcga ggatttgctc cattacaccg cgtactagat
    10921 taaccggaat cgcaaatccg atgccttcgg gtccgccgta ggcgcgattc agcacagcgg
    10981 tgttgattcc gaccagatcg ccttgcgcgt cgatgagcgc gccgcccgaa ttgccgagat
    11041 tgatcgcagc atcggtttga atgaaattct cgaaggtggc gagcccgagc tgaccgcggc
    11101 cggtggcgct cacgatgcct tgcgtgaccg tttggctgag accgtaggga ttgccgattg
    11161 cgagtacgat atcgcccacg cgcagcgtat cggagcgccc taccggcatg atgggtagct
    11221 tgccgatgtc cagatgcagg atggcgatat ccgtgtccgg gtcttggccg acgatgctcg
    11281 cgcccgcgat gcgcgcgtcg gcgagctgca ccttgatgga atcggcgccg gcgatgacgt
    11341 gctggttcgt cacgatggtt cccgcggcat cgacgatcac ccctgacccc aagctgcgtt
    11401 cgacacgctg gcgatagctc ggccagtagt cgccgaacag ctgactgagc gcgggtgatt
    11461 gagtgcgttc cgtaaccacg cgttcggtat aaatatttac cacggcaggc gaagcccgcg
    11521 cgaccgcatc ggcaaagctc tgcatgacca caatgggtcg ccccgcggcc ggcggagtgc
    11581 tctgcgccgg aagggtcgtc ttgcccaaga gcgcaggttt ccaccagacc acgagaaatg
    11641 ccgccgccag gccgacgatc acagctctga tcaagaaact cgcgaagacg cgagcatgct
    11701 gcaagtaatc cgctcctaag gcccttaagg gaaatccact tgtgtataat gcgctcccat
    11761 tattctttgg gaaacaccgc ggccggagag gtttgcgtga agatttgtca acatttcggg
    11821 ggcgattgaa tgagcgaaga cgttgaccac agccggcgcc gcttgttgac agcggccacg
    11881 gtcggcaccg gggcgatcgg cgttgcgttc gccgccgttc ctttcttggc atcgtggcaa
    11941 cccagcgcgc gcgccaaagc gctcggggct ccggtcgagg tggatgcgtc caagctcgag
    12001 gccggacaga tgttgaaagt ggtttggcgc ggccaagccg ttttcgtcgt gcgccgtgtc
    12061 aagggaatca tcgatcagtt gggcggccac gatgcgctgc tcgccgatcc gcagtccgac
    12121 gattcgatcc agcccgagta tataaaggcc accggtgcag cgcgcgcgcg caaccccgag
    12181 tattgggtcg gattggcggt atgcacccat ctcgggtgct cgccgttggg cgcgttcgaa
    12241 gcgaataatt ccttccaggt cgccggcgcc gatttgggag cgaactggcc gggcgggttc
    12301 tattgcccct gtcatggttc gaagtacgac ctgtcgggcc gcgtcttcaa gagcatgccg
    12361 gcgcccaaga acctgaccgt accaccttac gtgttcgcgg acggcgcgag aatcacaata
    12421 ggcgtcgacg acgcccccaa gaaagcttaa tagcgagagg ttgacgcgat ggccgcagca
    12481 cccgagtccg ccgacagcag caaaggcgga agtttgatcg gctggatcga tcagcggttt
    12541 ccgctgacca agctctggaa ggagcaagtc gccgagtact acgcaccgaa gaacttcaac
    12601 ttttggtact acttcggcgt tctcagcata gttgtgctcg tcaaccagct gatcaccggc
    12661 attttcctca cgatgaatta caaaccgtcg gccgccgagg cgttctcctc ggtcgaatac
    12721 atcatgcgag acgtcgactg gggctggttg atccgctata tgcactcgac cggcgcatcg
    12781 gcgtttttca tcgtgattta tctccacatg ttccgctcgc tgttgtacgg atcgcatcgc
    12841 aaacccaggg aactgatttg gattttcggc tgcctgattt tcgtgctgct gatggccgag
    12901 ggattcatgg gttatgtgct gccctggggc aacatgagct attggggcgc gcaggtcatc
    12961 gtctcgctat tcggcagtgt gccgtatgtc ggcgatacgc tggtcgaatg gattcgcggc
    13021 gattactaca tcgccgacgc caccttgaac cggttctttg cgctgcatgt tgcggcgttg
    13081 ccgttggcgt tggtattttt ggtgatcgcg cacttgatgg cgctgcacga ggtgggctcc
    13141 aataatccgg acggcatcga aatcaagaag tacaaggacg ccaacgggca tcccctcgac
    13201 ggcatcccgt ttcatcctta ctacacagtg aaggacacgg tcggcgtcgc cggctttttg
    13261 ctgttgtttt gcggcgtgat cttcttcgcg ccgacattcg gcggcctgtt cctggaatcg
    13321 ccgaacttcg agccggcgaa cgccttgcag actccgccac acatcgcgcc ggtgtggtat
    13381 ttcacgcctt tctacgccat gttgcgcgcc gttccgtcgt tcgccggcac gcaggtctgg
    13441 ggcgtgctgg tgatgggcgc ttcgatcgtc gtgctgtttt tcctgccatg gctcgaccgc
    13501 tcgccggtgc gctcgattcg ctaccgtggt ccgatataca agatcatgtt gaccatcttc
    13561 gtactttcct tcgtctgcct cggactctgc ggcctgaaac cgccgtccgg catttacccg
    13621 acgttggcca aaatctgcac ggttttgtat tttgctttct ttttgctgat gccctggtac
    13681 acgaaattcg agagcgtgaa gccggtgccg gaaagggtca cagtccatgg ttaaaagctg
    13741 ggtattcatt gctattgcgc tcatttccgc gggtaatgcc ctggccgagg agggcgacat
    13801 ggcgctgctg ccgtcaggca cggacatcaa gagcatggag tccctgcagc gcggcgctcg
    13861 caatttcatg aattattgct cgggctgcca ctcgctcaag taccagcgct acaaccggtt
    13921 ggcggcagac ctcaagattc ccgaatcgga actcacggcc gatttgatgt tcacttcgga
    13981 taaagcggtg gatacgatca attccgccat gccgaaggat gccgaggcct ggttcggcaa
    14041 gcagcccccc gacttgtcgc tgatggcccg cgagcgcggc acggactatt tgtattcgtt
    14101 tctcaagggc ttctacgccg acaagactcg tccctggggc gtgaacaatc tgtatctccc
    14161 gggcgctgcg atgccgcatg tcctgcagac attgcagggt ttacaaaagc cggtgttcaa
    14221 gaacgagccc gacgagcgcg gcagcgccag gatggtgttg gtgggggtgg atgcaatgac
    14281 tccgggcgcg ctcaagccgg aggagtatga tcagtttgtt cgggacatcg cgaatttcct
    14341 ggactatgtc ggtgagcccg tcaaggaaaa gcgccaatcg atgggcgttt ttgtgacgct
    14401 atttttgctg gtgttctttg tgttcgccta cttactgaag aaagagtact ggaaggacgt
    14461 tcactgacgc agcagcgggg attcccgtgt cttgctcctt aggagattgc gttagtggca
    14521 cgtcgctcgg tgatgaccct cttttccgca ccggctgacc cctggagtca ccggacgcgc
    14581 ttggtacttg ccgaaaaggg catcgccatt gagttggtca atgtcgacgt caacgatctt
    14641 ccggaagatc tgctcgatct gaacccctat cacagcgtgc ccactctggt ggatcgagac
    14701 ctggtgttgt acgactcgcg cgtcatcatc gaatacctgg atgagcggtt tccgcatccg
    14761 ccgttgatgc ctatcgatcc ggtgacccgc gctcaatttc gcgtcgcgct gtatcgggtg
    14821 gagcgcgact ggtacgcgtt ggcccgcgac atcgagttgg acccgcagag caagaccgcg
    14881 gcacacagcc gcaaggtcct gggcgaggcg atttgcgcca gcgccgatgt attcaaggcc
    14941 aagccgtttt tcctcagcga cgagttttcc ctggtcgatg ccagcatcgc accgatactc
    15001 tggcgcttgc cgagttacgg catcgagctg ccggtgcaag ctcagccgat cacgaagtac
    15061 atgaattcga ttttctcgcg ctcgactttc cgctcctgcc tcaccgaagt cgagcggggt
    15121 atgcgacatt gaagccgcga cgcccttacc tgttgcgtgc cctgcacgag tggatttccg
    15181 atagcggcga gacgccgcac atcgtggtgg acgcggccgc cgaaggggtc agcgtgccgc
    15241 gccaatacgt caaagacggt aaaatcgtcc tcaacgtgag tttgtcggcg acgcagatgc
    15301 taaagctcgg caacgacgtg gtgagcttcg aggcgcgctt cggcggcgtt tcattcgccg
    15361 tccaggttcc ggtgcgggcg atcttgggga tttacgcacg cgaaaccggc cagggcatga
    15421 tatttcccga aggtgatgcc gatcccgatc cgagcgacgt gccgccgccc ggtccggcgg
    15481 ccggtaagga cagcggctcc aaagcgaagc gcccgaaact gcaagtcgtc aaatagaacg
    15541 aaaggcgtcc tttatccaga cgatgtgctg cgggccctcg gctggaccct gcacgccgac
    15601 gacgtcgaag cgcatcataa gaccacgcca tgcggcgcgc ctcaacaaga agtatcgcgc
    15661 ggcgcgcatc agtttgcgct gcttgcggtg gtcgaccgag gcgagcgcgc cgccgtagtc
    15721 gatgcgcatg cgctgtctga cttcgacgat cgcgagcact ccgcgatcga ggcacacgag
    15781 gtcgagttcg ccgagtttgc agcgcaagtt gcgcgccaga atccgcagac cctggctctc
    15841 gagatatgcg gccgcgagtt gttctgcgac cgcaccgcgc ttccaggctt gatcggacac
    15901 gggcgtcaga atgcaggccc aagcgccgtg cgtcagcccg ggaatgcgcc tgcttcgtgg
    15961 caaaatgcgc ggatgcccga atatcgcaac aattacatag gatcggtcac caaatcgctg
    16021 ggcgagctgt tgcgctggca attcgagcga gtccgcagcc ggctgccgcc ggcgcccacc
    16081 gtgccgatac cgacggtcgc cgcggatctt gacttcgtgc agggaaatgc cgtcgcggct
    16141 ggcgccatga ttccggcggt cacgtggatc ggtcatgcga gcgtgctggt gcaggcctgc
    16201 ggcttgaacg tgctgaccga cccgatattt tccagccgcg catcgccggt gcaattcgcc
    16261 ggacccgcgc gactgcaggc gccgggtatc gctttgcgcg acttgccgca catcgacgtc
    16321 gtggtcattt cgcacaacca ttacgatcat ctggaccgcg gcagcattca aacgctcgcg
    16381 cgccaggccg gcggcccgcc gttattctta gtgccgctcg gaatcaaggc gtggctcaag
    16441 cgcgccggga ttgagcgagc ggtcgaactc aattgggggc agatgcaggt ccacgagcag
    16501 gtcgaatttc atttcacgcc cgcgcagcac tggtcgggcc gctgcctcag cgaccgcaat
    16561 cgcaccttgt ggggtagttg ggccgtgctc ggcgctcagc tgcattggtt tttcagcggc
    16621 gacacgggct atagcaagga ctttcgcgac attcgcgagc gattcgcgcc gcgccaaacc
    16681 acggcacagg gcggtgcctt cgatattgcg ctgattgccg tcggcgcatg tctgccgcgc
    16741 tggttcatga aggagcaaca cgtggatctc gatgaggcca tgcaaatcca cttggacctc
    16801 ggcgcgaaac gcagcatcgg tgtgcattgg ggaacattct cgctcgccga cgatccgccc
    16861 gacctgccgc tgcacgagtt ggcgcatact cggcgcggta aaggggtggc ggacgaggaa
    16921 ttttttctgc tgcccgtcgg cgggactcgg cggctggcca gacgcctgga ggcacatcga
    16981 tgacaaactc gcttttggca ggctgggaca acttctacgt catcaccggc tcgtcggcag
    17041 cggggctcac cggactcact ttcgtcgtca tcgcgctcgc cgccgaggcc aaacgcgtga
    17101 gtctcaaagg cctgcgcacg tacgtcacgc ccacgatcgt gcacttcggc gccgtgctcg
    17161 cgctcgccgc ctatctcagc atgccgaacc agggcgtact cagcttaagt ctcgggtttg
    17221 gcgcagcagc atgtgcgggg ctcgcctatg tcggctccat cgcagcgagt atcggacgca
    17281 tcagcaagga ttacatgccg gtggccgagg attggatctg gaatgtgatc ctgcccacaa
    17341 tagtttacgg aactctgctc gggatggcgg ttctcatttg gacgaggcca gaggtcagca
    17401 tgtacggggt agccgcggta ttggtgctct tgatgttcat cggcattcac aacgcgtggg
    17461 atattgccgt ctggaacagc gtccgcaagc tggatgattc ggcctagaac tgtcttatgt
    17521 cttattacgg cgacattttc gcgccgcgaa atcttggtct aatatcgagt tcaccagatc
    17581 aaaaacaggc gttttgcgat gaattccgca gttgacaacg tattgcacca acttcagcta
    17641 ggcgaagatc tcattgccga tggaatcgaa gtgctcgctc ggcactggca agcttcgacc
    17701 accgcgatcg gcaagttgtg tacgggatcg gatcgttggc aggcggcgct gcgggaaatc
    17761 cagacgcagg tgaatctcgc ggaaatcttc cgcggccgta ccatcatcta gttcgcggct
    17821 gcccgaacgg gcgtcgcgct cgagtcgaat accggttcac cgccgaccca agtcttcagc
    17881 acgtgaacct cgcggatcct ggccgcggga attgcgagca cgtcctcgct gagcagcacg
    17941 aggtcggcga gcttgcccac ctcgatggac cccttgtcgt gttcctggaa ttccgcgtac
    18001 gccgatccca gggtgtaggc gaatatcgcc tcttgaatgc tgagtttttg ctccgcaaac
    18061 cagccctccg gatgcttgcc gtcaagcgtt gcgcgggtag tcgcggcgta cagcgtcagc
    18121 atcggatcga gcggtgcgac cggccaatcc gtgccgaatg cgaggcgcac gccgtgatcc
    18181 aggaaagtcc tgaaggcgta agttctactc gcacgttcat gtccgatgcg cgcttcggcc
    18241 cagcgtccat cgtcgatcgc ctgataaggc tgtaccgagg cgatcacatg cagttgcgcg
    18301 aagcgatcga aatcgcgcgc agccatgtgc tgcgcatgct cgatccgcca gcggcggtcg
    18361 cgtggcccgt cggcggttgc gacgtcgtcg tacagatcca agacggtgga tatggcggca
    18421 tcgccgatgg catgagtaca aacctgcaaa ccggcggcat cggcgcgcag caggcgctgg
    18481 ccggtccgcc cgggcggcag catgtcctcc gccagcaacc cgcgaatatc cggcgtatcg
    18541 agaaagggtt cgaagaaata agcggtgcgc gagcccagcg atccgtcggc gaacatcttg
    18601 agcgcgccga ttctcaagaa cgggccgccg aaagcccggc cgataccgag ccgcgcctgg
    18661 tcgtccaccg ctttcaccgg tggcgcggcg tacagccgca ccgtgagctt gccgtcgcgc
    18721 aacagctccg agtacaccgc caggttggcg taatcctcgc gcatgtcctg cacgctggtg
    18781 acgccgcgcc tcgcggcttc ccgcagcgcc ccctcgattg cgcggcgccg ttgtgccgcg
    18841 gtcggctcgg gaatcactct ctcgaccaat ccggccgcag catccgcaag cgcgcccgtc
    18901 ggttgtccgg ccgcgtcgcg cacgatcacg cctcccggag gattgggggt tgcggctgtg
    18961 atgcccgcga gcgccaaggc cttggagttg accaggatca tatgcccgtc atagcggtcg
    19021 atggctagcg gattgttggg cgttacggcg tcaatatcct ggcgggtcgg taatttggct
    19081 gggctccact ttgtctcgtc ccagtcgccg gcgagtatcc attcgccttt tgccgcgcgc
    19141 gcggcgtagt cgccgatgcg cttgacgaac acctcgaggc tgggtgcatc gttcagttgc
    19201 acggcgctca gggaggcgcc gccgtcctcg aagtgaacgt gcgcatcgtt gaagcccggc
    19261 aacagccgct tgccttcagc atcgacgacc tgcgtgcgtg acccagccca agtcagcacg
    19321 gcgccgtcgg agccgacagc gactatccgg ccgttgagga cggccacagc ctgggcctct
    19381 gcctgggccg ggttcaaggt ccaaatacgc gcgtggcgga tgatgagatc cgctgcgggc
    19441 ttatcgggcg aggccgcatg agcgaccgcc agagccccgg acaggacggc cagcgcgccg
    19501 cagcgggcaa tgtcgacggc aatcatagtc cggccggctc cccatttttg atttgcgccc
    19561 actgcaactc acggtggatg cggttgcggt cgtcgaggtg cagcttgccg gtgacccccg
    19621 agatctcgct ggcttcggcc ggcgatttcg agcgcaatgc aggcaccagg cgataagcgt
    19681 cgaagccgaa ggcatacaat cgatcgcggc gcgcggtgcg cgccggccat gccgcgcgca
    19741 cgccgtcgcg aatttgcgtg gtgaccggat catccgcgac catccagggc atgtcgggaa
    19801 acagcatgcc gtcaagatcg ctgttggcgg cggagtcggg ttcgaaacta tccgaagtgg
    19861 agtacacc
//
LOCUS       DASWZT010000540        11861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_33608, whole genome shotgun sequence.
ACCESSION   DASWZT010000540 DASWZT010000000
VERSION     DASWZT010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11861
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33608"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VGE92_15395"
     CDS             complement(<1..412)
                     /locus_tag="VGE92_15395"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1315265.1"
                     /translation="MHPLIRRAYRSDAALVSRLAESTFRETFAGANTPQNMDLHCRDS
                     YSEELQAGEIAGQNMVTLVAEQGQDLVGFAQLHWSAAPSFVQGTSPGEIQRLYVASNW
                     HGKGVAQALMAACLEEMSARGADVAWLGVWERNPR"
     gene            515..1396
                     /locus_tag="VGE92_15400"
     CDS             515..1396
                     /locus_tag="VGE92_15400"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY1315266.1"
                     /translation="MDLERLEACSLCRSPNLVTVDAERYFCRCSDCGYIFDNPRPTAA
                     EIVRFYSKPGQYRDWLEEEVARDRMWQRRLRKLRRAGLSGSLLDVGSGTGQFLHHAKA
                     TFSQVLGTEVSTEAVQVARRKYDIELLASEFESSEFPEQFDVVTLFHVLEHVPDPRRT
                     VTRCYQVLRPGGMLIIAVPNDVQSLKQRLRRFLGRIPLLKPRIAGPLALPRLTLEKVG
                     QEVHLSHFTPTVLEGFLKRLGFTDVQTALDPYYARSGFRELRADLRYAVFSMINSVLR
                     QNLYSTIWVQARRPPIS"
     gene            1462..2553
                     /locus_tag="VGE92_15405"
     CDS             1462..2553
                     /locus_tag="VGE92_15405"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY1315267.1"
                     /translation="MHQPIADARLWNRSPLVPLAALWATLWTLVAIIESSYYIHNPSI
                     PPWQPLTLILSLTCTIALWLTFELRSDRYLRFPLDPPRPWFLYHLRRLPMLAAGYIVI
                     VFGLRHAVFAMAGVRYDHLPWQGQIPFELAKASLFYCLWLGLVFGTLSLLKSREQSAQ
                     LDQVQKALLESQLAQLQAQLRPHFLFNTLNTVSSLMQIDIARADRVLARLADLLRASL
                     SAASTSTVALRQELSLLHLYADIMQERFSGRVQVEWQIAENATAIPVPAMILQPLLEN
                     AFRYGIELTTGVENIWVVATLARGQLIVTIRNTGSTLRDGWRAGVGITNCRERLRVLY
                     GDAAGIDLSNHAAGGVLASVSVPLGAVIE"
     gene            2550..3290
                     /locus_tag="VGE92_15410"
     CDS             2550..3290
                     /locus_tag="VGE92_15410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY1315268.1"
                     /translation="MIRAIIVDDEEPARAKLRRWLRSEPDIEVAGEYADGLSAAQSIM
                     RDSPDVVFLDIQMPGLSGLQVAAQLELDGAPLLVFVTAFDAHALAAFDLGAVDYLLKP
                     YDEERFRRALGRIRGRLHAPEHRRSAVRVARQQIGVSDRLLVPAGDGLRMIDSAGIHS
                     LEADDNYVHVRTADQTYLLRRTLRDLLQQLGEQRFAQIHKSVAVNIGEIDALTPLFKG
                     DYELRLRSGRLLRLSRRYTTQLFSRTGR"
     gene            3394..4071
                     /locus_tag="VGE92_15415"
     CDS             3394..4071
                     /locus_tag="VGE92_15415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1315269.1"
                     /translation="MTGVTKAYASMAGPIVALRDVSLEVRTGEFVAVIGKSGSGKTTL
                     INLLTGLDSPSTGTIRVASTDVHTLDQEGLSNWRGLSVGIVFQFFQLLPSLSIAENVM
                     LPMDFCNSYPRRERRSRALELLARMGIADQADKLPTALSGGQQQRAAIARAVANDPPL
                     IVADEPTGNLDSHTAEDVMQFFAHLVTAGKTVVMVTHERDLARYFARSITLEDGTIAS
                     QDAPRCA"
     gene            4068..6470
                     /locus_tag="VGE92_15420"
     CDS             4068..6470
                     /locus_tag="VGE92_15420"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1315270.1"
                     /translation="MSPRWRKLRGDIELAKGRLSMMIIAIAVGVFAVAAISAAYTILT
                     REIVRNYLSTNPAAALLDVDTIDERLLQGVRAQPGITGTEAAGRLTGRIEVRPNEWLP
                     LLLLVVPDFRAGRIGTVRLEAGQWPTASNGIVLERTALSVANTAIDREIRVQTRDGAQ
                     RSLAVTGVVHDPSLAPAWQEQTVYGYVTPATLRFLGVEPSLHLLKVTVSDAWRDPAGV
                     ERAVIGVAEWLRRSGQSVGEIRIPPYQHPHQGMMTSVVRMLLVFSVLTLILSAILTAT
                     LTSSLLAPQARQIGVMKAIGARSAQIMQIYVGLICAIGILAVTLGLPFGIAAGRALAH
                     STAQMLNLELASRSVPAWLYATQVLIGVTLPLAAALRPIHRAAGLTVRETLSDFGARL
                     AVGPGRRIRWLSRLIGRDPALVLAIRNTARRKGRLALTLGLLATAGAMFMTSLNVKAA
                     WQRNLTLAGMERHFDAQFQFARAQPQAATLAAVAAVPGVQRVEPWSIEAASRARADGL
                     RIVRTYPDGGHGSLRLQAVPRDSAFLSPTLIAGQWLSAPDSDGAVVNEQALSMFPGLR
                     VGDKLPLTVRGFVADLRVVGVVREHLSQATVYTSPERFAQETGQPGLTGGVRVALQSI
                     DAHSAEVSMSSIERALTGSGVAVSQSISQAQLHRALGGHLFILIFTLMVMSILMASVA
                     MMGLGSAMTIGVLERTREFAVMRVIGASAWTLRRTVVGEALFIAALSAGVSLALSVPL
                     TIFVEWLVGAASFGPALGTALSAAALPLWLAITLTGAVAASAYPSWRASKLTIREALG
                     FQ"
     gene            complement(6935..7192)
                     /locus_tag="VGE92_15425"
     CDS             complement(6935..7192)
                     /locus_tag="VGE92_15425"
                     /inference="COORDINATES: protein motif:HMM:NF018500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HEY1315271.1"
                     /translation="MDEQRPASVMVPHTELEPDLLHAVVESYVLREGTDYGEREVSLE
                     AKVSQVIGQLQRGEAGIVFDPETETVSIVRSQERFEVKTPR"
     gene            complement(7192..7650)
                     /locus_tag="VGE92_15430"
     CDS             complement(7192..7650)
                     /locus_tag="VGE92_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015378681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEY1315272.1"
                     /translation="MATGIWVDADACPKVIKEILFRAAERTGVRVTLVANQPMQIPRI
                     ASVRAVQVASGFDVADNEIVKRVAVGDLVITADIPLAAEVIAKGGAALNPRGERYSPD
                     TIRGVLGMRDFMDTMRASGNVGGGPPPLTQADRNAFAGQLDTWLARRQGA"
     gene            7741..8820
                     /locus_tag="VGE92_15435"
     CDS             7741..8820
                     /locus_tag="VGE92_15435"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY1315273.1"
                     /translation="MELKAEALIIGAGPAGLAVAACLKKIGAPFLIVEQAATIGSTWR
                     RHYDRLHLHSDRDHSALPYLDFPRGTPRYPSRDQVIAYLEFYARHFGLAPRLGERVQS
                     VQPGEAGWVTITAAGAYRSRHVIVATGCNAVPNLPRWPGQEHFRGRILHSGEYRDGEP
                     FRDRNVLIVGFGNSAGEIAIDLYEHGAHVAMAVRGPVNIIPREILGLPILTVSLAFSR
                     LPPRLADALAAPLVRLTIGDIARLGFRKPLCGPITQINTTARIPLIDIGTVKLIREGR
                     IEIRRGLRSISQDAVTFDDGSTRRCDAIVLATGFRAGFDRFLNQGRLAAPAGLHFCGF
                     RVSPTGMLRDIGLEARQIAERISTH"
     gene            8928..9452
                     /locus_tag="VGE92_15440"
     CDS             8928..9452
                     /locus_tag="VGE92_15440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY1315274.1"
                     /translation="MPDNRSFVANIAARYGPRLRRFLSVRLRNAADVPDLAQEVFLRL
                     LRVDGSESIRSPEAYLFTIASHVIHQHAVRRSSEPVSVDIAEVFSELRTPSSDDPPDQ
                     VAHAQRLDELERVLAYLPARVATALVLHRVAGYSVQEVGDELGVSRETAKKYLARAVE
                     HCRNLRASEGATRL"
     gene            9449..10504
                     /locus_tag="VGE92_15445"
     CDS             9449..10504
                     /locus_tag="VGE92_15445"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEY1315275.1"
                     /translation="MRIGPASSKTNPVIVTEAAAWFIEFRAGDVNGEARLRFIDWLRR
                     SPEHIQAYLEISGVWAELPTADPEGKLDIAALIARARNEADVIALPALRPSIANLPLH
                     ENSPTFWQRPRRALLAMAALGLLAAVLLRYVGNDLTDSYATGIGEQRTIQLSDGSTVE
                     LNARSRVQVQLTNRQRNVSLLEGQALFRVAKDKQRPFVVSAGDAQVRAVGTEFDVYRK
                     QVSTVVTVVEGRVETFDPSASAGAAPIVLSAGEQLTVLPHTVTKPTKTDTLVATAWVQ
                     RRLMFEETPLSEVAEEFNRYNRRPLTIDDRDLQKIKISGVYSSTDPASLINFLRSQGS
                     IQVIETENQVRVVRRAR"
     gene            10603..>11861
                     /locus_tag="VGE92_15450"
     CDS             10603..>11861
                     /locus_tag="VGE92_15450"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY1315276.1"
                     /translation="MRIAVVTAAVCLSIVGLSLADGANAAIRKPTHIAAQGLGPALKQ
                     LAQSRGLQVLYLSSTVRDLRTRGANGDITADEAFDQLLTGTGLTYRYLDENTVTVVPL
                     SAPALPDGAGVLLQAPDGDAVATAAAISDVTNVSADKYLLAQNVPASAQASSATEAES
                     PPLQEIIVTGSRIASPNEVSTSPIQVISSKSIQVSGKTDITDIISQLPQIFTNDLGQD
                     LGNGTSGLTTAGGVATADLRGLGPGRTLVLVDGRRLGVGSPNTAIAQPAPDLDQIPAG
                     LVERVEVVTGGASAAYGSDAIAGVVNFIMKRNFEGFQVDGQVGENYHDNNDAFMQNLV
                     RQFGYTPPTGTEQDGRNRTFDVLMGTNFADGNGNVTGYLSYRHADPVVASQRDFGACQ
                     LFPTYDNAGNVTGVTCGGSSNSNWFEPT"
BASE COUNT         2082 a         3847 c         3602 g         2330 t
ORIGIN      
        1 ctcttggatt gcgttcccag acaccgagcc acgcgacgtc cgcgccgcgg gcactcattt
       61 cctcgaggca cgcggccatc agcgcctgcg ccacgccctt cccatgccaa ttgctcgcga
      121 cgtataatcg ttggatctca ccaggcgaag tgccttgaac gaaactcggc gctgcgctcc
      181 aatgcaattg cgcgaacccc accagatctt gcccttgctc agccaccagc gtaaccatgt
      241 tctggccggc gatctccccc gcctggagtt cttcgctgta gctgtcacgg cagtgcagat
      301 ccatgttctg cggcgtattc gcgccagcaa acgtctccct gaaagtgctt tcggcgaggc
      361 gcgacaccaa tgcggcgtcg gatcgatagg ccctgcgaat gagcgggtgc atgctctcag
      421 catagcaagc tgcgggattt gggccaaacg cggccgtcgt cggcccgcag cttttgtggc
      481 agtctaatcg cgcgacttgc caagtgggga atgaatggat ctagaacgac tcgaggcgtg
      541 cagtctgtgc cgatcgccga accttgtgac ggtcgatgct gaacgctatt tttgccgctg
      601 tagcgactgc ggctacatct tcgacaaccc gcgcccgact gcggctgaaa ttgttcggtt
      661 ctactcgaag ccgggacagt atcgcgattg gttggaggag gaagtggcgc gcgatcgcat
      721 gtggcagcgg cggctgcgta agctgcgtcg cgccggactt tccggctcgt tgttggacgt
      781 cgggtcggga accgggcaat tcttgcacca cgccaaagcc acattctcgc aagtgctcgg
      841 cactgaggtc tccaccgaag ccgtgcaggt ggcgcgccgc aagtacgaca tcgagttgct
      901 tgccagcgag ttcgagagca gcgaatttcc tgaacaattc gacgtggtga cgctcttcca
      961 cgtgctcgag catgtgcccg acccaagacg caccgtgacc cgttgttacc aagtgctacg
     1021 ccccggtgga atgctcatta tcgcggttcc gaacgacgtg cagtcgctca agcagaggct
     1081 gcggcggttt ctgggcagaa tcccactgct caagccgcgc attgcgggac cgctggcatt
     1141 gccgcgtttg acgctggaga aagtcgggca ggaagtgcat ctttcccatt tcaccccgac
     1201 cgtgctcgaa ggcttcctca agcgcttggg cttcacggac gtgcaaacgg ctctcgaccc
     1261 atattacgcc cgcagcggct tcagggaatt gcgtgcagat cttcggtacg cggtgttcag
     1321 catgatcaat tcagtgttga ggcagaacct ctacagcacg atctgggtgc aagcacgtcg
     1381 cccaccgatc tcatagcgct cgcgccccgg tcaccccaat ttactcgcgc tcgtccgggc
     1441 aggcattaga ctgccaagca tatgcatcaa cccattgccg acgcgcgcct ctggaaccgc
     1501 tcgccgctcg tcccgctcgc ggctttgtgg gcgacgctgt ggacgctcgt cgccatcatt
     1561 gaaagctcct actatattca taacccctcg attccgcctt ggcaaccgct caccctgatc
     1621 ctatcgttga cctgcacgat cgcgctctgg ttgacgttcg aacttcgttc ggaccgctat
     1681 ctgcgattcc cactggatcc gccgcggccg tggtttttat atcacctgcg gcgcttgccg
     1741 atgctcgcgg ccggctacat cgtgattgtc ttcggcttac gccacgcggt attcgccatg
     1801 gccggcgttc gctatgacca cttgccatgg caaggacaga ttcccttcga attggccaag
     1861 gcatcgctct tctattgtct atggctggga ctcgtctttg gaacactgtc gttgctcaag
     1921 tcacgcgaac aaagcgcgca gcttgaccag gttcaaaagg cgctgctgga gtctcaactc
     1981 gcgcaacttc aggcgcagct ccgaccgcat tttcttttca atacactcaa caccgtctcc
     2041 tcgctcatgc aaatcgatat tgcgcgggcg gatcgggtct tggcgcgact cgcagatctg
     2101 ttgcgcgcca gtctgagcgc cgcaagcacc agcaccgttg cgctgcgtca ggagctgagc
     2161 ctgctgcatc tgtacgcgga catcatgcag gagcgcttct cgggcagggt gcaggtcgaa
     2221 tggcaaatcg cggaaaatgc gactgccatt cccgtgccgg cgatgatctt gcagccgctg
     2281 ttggaaaatg ccttccgcta tgggattgag ctgacgaccg gcgtcgaaaa catttgggtg
     2341 gttgcgaccc ttgcgcgcgg ccagctcatc gtcacaatac gcaatacagg ctcgacgctg
     2401 cgcgacggct ggcgcgcggg ggtcggcatc accaactgtc gcgagcgact gcgcgtgctg
     2461 tacggcgacg ctgccggtat cgacctgtcg aatcacgccg caggcggcgt gctcgcgtcg
     2521 gtttccgtgc cgttgggcgc ggtgatcgaa tgattcgcgc catcatcgtc gacgacgaag
     2581 aacccgcgcg cgccaaattg cgtcgctggc tgcgcagtga acccgacatt gaggtggccg
     2641 gcgaatatgc cgacggcttg agtgccgccc agtcgatcat gcgggactcg ccggacgtgg
     2701 tattcctgga cattcagatg ccggggttga gcggtctgca agttgccgcg caactcgagt
     2761 tggacggcgc gccgctcctc gtattcgtca ccgccttcga tgcgcatgcg ctcgccgcat
     2821 tcgatctcgg ggcggtcgat tatctgttga agccgtatga cgaagagcgc ttccgccgtg
     2881 cgctcggtcg catccgcggc cgcttgcacg cgcctgaaca ccgtcgatcg gccgttcgcg
     2941 tggcgcggca gcaaatcgga gtcagcgacc ggctgttggt tcccgccggc gatggactgc
     3001 gcatgatcga cagcgccggc attcatagtc tcgaggccga cgacaactac gtgcatgtcc
     3061 gaaccgccga tcaaacatac ctgctgcgaa gaacccttcg cgaccttctt caacagctcg
     3121 gggagcagcg tttcgcgcaa atccacaaat cggttgcggt caacatcggc gagattgacg
     3181 cgctgacgcc gctcttcaag ggcgactatg aattgcgact acgctccgga agactcttgc
     3241 gtctgagccg gcgctacacg acgcagcttt tcagccgcac gggacgttag cgcccccgct
     3301 caccctcgcc gcgctccgct tgccccgtaa gggtcgcgac ttgcggcgct ttgcaatacc
     3361 gtctacgcaa tgtcactgcg cccgctgatt tccatgaccg gtgttaccaa agcctacgcg
     3421 agcatggccg gccctattgt cgcgttgcgc gatgtgagtc tggaggtccg gacgggcgag
     3481 ttcgttgccg tcatcggcaa gtcaggcagc ggcaagacca cgctgatcaa tctcctcacg
     3541 gggcttgact cgccgtcgac cgggacgatt cgggtggcat cgacggacgt gcacacattg
     3601 gatcaagagg gactctcgaa ctggagaggc ctgtcagtcg gcatcgtatt tcaattcttt
     3661 cagctgctgc cctcgctcag catcgcggag aacgtcatgc tgccgatgga tttctgcaat
     3721 tcctatcctc ggcgcgagcg acgttcgcgt gccctggagc ttttggcacg gatgggcatc
     3781 gcggatcagg ccgataaact gccgacggcg ctctccggcg gccagcagca gcgcgccgcc
     3841 atcgcgcgcg ccgtggcgaa cgatccgccg ctgatagtcg ccgatgagcc gaccgggaat
     3901 ctcgattcgc acacggcgga agacgtgatg cagttctttg cgcacctggt gacggccgga
     3961 aaaaccgtgg tcatggtgac gcatgagcgc gacctcgccc gctactttgc acgctcgatc
     4021 acgctcgagg atggaacaat cgcgagccaa gacgcgccgc gatgtgcttg agcccgcgtt
     4081 ggagaaagct ccgcggcgac atcgaactcg cgaaaggccg gctgtcgatg atgatcattg
     4141 ccatcgccgt gggagtcttc gccgtggcag ccatctcggc cgcttacacg atcctgacgc
     4201 gcgagattgt tcgcaactat ctgtccacga accctgccgc cgcgcttctc gacgtggata
     4261 cgatcgatga gcggttgctt caaggagtca gagcccaacc cggaatcacg ggcaccgagg
     4321 ccgcaggccg gctgaccggg cgcatcgagg ttcggccgaa tgagtggctg ccgttgctgc
     4381 tgttggtcgt gccggacttc cgtgcagggc gcatcggcac cgttcgcctc gaggccgggc
     4441 agtggccgac ggcgagcaac ggcatcgttc tcgagcgcac ggcgctgtcg gtggcgaaca
     4501 cagccatcga tcgcgagatc cgcgtgcaga ctcgcgacgg cgcgcaacgc tcgctcgcgg
     4561 tcacgggagt ggtccatgac cctagcctcg ctcccgcgtg gcaagagcag accgtgtatg
     4621 gctatgtgac gcccgcgacg ctgcgcttct tgggcgtgga gccgtcactt cacctgctga
     4681 aagtgacggt atcggatgca tggcgtgatc cggccggagt cgagcgcgcc gtcattggcg
     4741 ttgctgaatg gctgcggcgt agcgggcaat cggtcggcga gatccgaatt ccgccttacc
     4801 aacatccgca ccagggcatg atgaccagcg tggtgcggat gctgctcgtg ttcagtgtgc
     4861 tgactctcat cctgagcgcc atcctcacgg cgacgttgac gtcgagcttg ctcgcccccc
     4921 aagcccggca gatcggcgtg atgaaggcga tcggcgcgcg cagcgctcag atcatgcaaa
     4981 tctatgtggg gttgatctgc gcgatcggca tcttggccgt tactctcggc ttaccgttcg
     5041 gcatcgccgc gggacgggct ctggcgcaca gcaccgcgca aatgctcaac ctcgagttgg
     5101 cgagccgatc ggtccccgcg tggctctacg ccacccaggt gctcatcgga gtgaccctgc
     5161 cactggcggc ggcgctgcga ccgatccacc gtgccgccgg gcttaccgtc agagaaacgc
     5221 tgagtgactt tggcgcgcgc cttgcagtcg gccccggacg acggattcgc tggctctcgc
     5281 ggctgatcgg tcgggatcct gcgctcgtgc tcgccattcg caacaccgcc cgacgcaagg
     5341 gccggctcgc cttgactctc gggctcctcg ccacggcggg ggcgatgttc atgaccagtc
     5401 tcaacgtcaa agcggcctgg caacgaaacc tcacgctggc cggcatggag cggcacttcg
     5461 acgcgcagtt tcagttcgcg cgtgcgcagc cgcaagcagc cacgctcgct gccgtggctg
     5521 cggtgcccgg tgttcagcgg gtcgagccct ggagcatcga agcggcctcg cgtgcccgcg
     5581 cggatggtct gcgcattgtc aggacctacc ccgatggcgg ccacggaagc ttgcgacttc
     5641 aagccgtgcc ccgggacagc gcattcctga gcccaacgct gattgccggt cagtggctga
     5701 gcgcgcccga tagcgacggc gccgtggtca acgagcaagc cctgtcgatg ttcccgggat
     5761 tgagggtcgg cgataagctg cctctcaccg tccgcggctt cgttgccgac cttcgcgtcg
     5821 tgggagtcgt gcgggagcat ctgtcccaag cgacggtgta tacctcgccc gagcggttcg
     5881 cacaggaaac cggccaacct ggcttgactg gaggcgtgcg cgttgccttg cagagcatcg
     5941 atgcgcactc cgccgaggta tccatgtcga gcatcgagcg cgcgctgact ggctcaggcg
     6001 tcgctgtaag ccagtcaatc tcgcaggcgc agctgcatcg tgccctcggc ggtcatttgt
     6061 tcattttgat cttcaccttg atggtgatgt cgatactcat ggcgagcgtc gcaatgatgg
     6121 ggctgggctc ggccatgacg atcggcgttc tggagagaac tcgcgaattc gccgtcatgc
     6181 gcgtcatcgg cgcgagcgcc tggacattga ggcgaaccgt tgtcggcgaa gccctgttca
     6241 tcgccgctct gagcgccggc gtctcgctgg cgctatccgt gcccctgacg atattcgtcg
     6301 aatggctcgt aggggccgcc tcgttcgggc ccgcgctcgg taccgcttta tcggcggcgg
     6361 ccttgcccct gtggcttgcg atcaccctga cgggtgccgt ggcggcgagc gcttacccat
     6421 cttggcgagc atccaaactg accattcgcg aagcactggg cttccagtaa ggggcgtttg
     6481 acgtgaaaaa aaccctcaac atccttgtcg tcgtattaat cctggcggcg ataatggcag
     6541 tactcatcca tcgcctaccg agcgttggcg acttgctgcg taagatccat ggcggctgat
     6601 ctcagcgcga cacgagtccg tccatgtccg cttgcatgcg ctgtttgtat tcatccgacc
     6661 aatgtgcgcc ggttttctgt ttgttttgat tgcgcgacgg cgcgaccggc accgcctgga
     6721 tttcatccgg ggccttccgt gcgccagcct ttagcttgag tgtcgggcga gatgccgcgg
     6781 gtgcggggat tgggttcgaa tcattcatgc ggttgtcggc tgcctcttca atgtgttgac
     6841 gtccgggagg aattgtactc aatgaggccg cgtacgttcg acctgttcgg cggcaatttg
     6901 tgtaagactc ttccggacaa gcgctgtcga tgactcagcg cggcgttttg acctcgaagc
     6961 gttcttggga tctgacgatg ctcacggtct cggtctcggg atcgaatacg atccccgcct
     7021 cgccgcgctg caactgaccg atgacctgcg acaccttggc ctcgagcgaa acttcccttt
     7081 ccccgtaatc cgtgccttcg cgcaatacat aggattcgac cacggcatgc agcaagtccg
     7141 gctcgagttc cgtgtgcggc accataaccg atgcgggtcg ttgctcgtcc actaggcgcc
     7201 ctgacggcgt gccagccacg tatcgagctg acccgcgaat gcgttgcgat ctgcctgagt
     7261 cagcggtggc gggccgccgc cgacattgcc gctggcgcgc atggtgtcca taaaatcgcg
     7321 catgccgagt actccgcgaa tggtatcggg ggaataacgt tcgccgcgcg ggttgagcgc
     7381 tgcgccgccc ttcgcgatca cttccgcggc caacgggata tcggcggtga tcaccagatc
     7441 cccgactgcc acgcgcttga cgatctcgtt gtcggcgacg tcaaagcccg acgcaacctg
     7501 gacggcgcgc acgcttgcga tgcgcggaat ctgcatcggc tggttggcca ccagcgtgac
     7561 gcgcacgccg gttcgctcgg ctgcgcggaa tagaatctcc ttgatcacct ttggacaagc
     7621 atcagcatcg acccagatac ccgtcgccac gtatcaccct cgcctcgcag ccgcaattga
     7681 tcacccaata tacacgcttg gccttgcccg ccgcggctac gactggcgac aatgctccgc
     7741 gtggagctca aagcagaagc gttgatcatc ggcgccgggc ccgcagggct cgcggttgct
     7801 gcctgcctca aaaaaatcgg tgcgccgttt ctgatcgtcg agcaggcggc cacgatcgga
     7861 tcgacttggc gccggcatta tgatcgactg cacctgcatt cggatcgcga ccactcggcc
     7921 ctgccgtatc tcgactttcc tcgaggcaca ccgcgctatc cgtcgcgcga tcaagtgatt
     7981 gcttatttgg agttttacgc acggcatttc ggacttgctc cgcgtcttgg tgagcgagtc
     8041 cagagcgtgc agcccggtga ggcgggctgg gtgacgatca ccgccgccgg cgcgtaccgt
     8101 tcccggcacg tcatcgtggc gaccggctgc aacgcggtcc ccaatctgcc ccgctggccc
     8161 ggtcaggagc atttcagagg gcgcatcctg catagcggcg agtaccgcga tggcgaaccc
     8221 ttccgggatc ggaacgtctt gatcgtagga ttcggcaaca gcgcgggcga aatcgcgatc
     8281 gatctatacg agcacggcgc gcacgtcgcg atggccgtgc gcggtcccgt gaacatcatc
     8341 ccgcgcgaga tcttgggctt gcccatcctc accgtctccc tggcgttcag ccggctgccg
     8401 ccccggctcg ccgatgcgtt ggccgcaccg ctcgtgcgcc tgaccatcgg cgacatcgcg
     8461 aggctcggtt ttcgcaagcc tctctgtgga ccgatcacgc agatcaacac gacggcgcgc
     8521 attccgctca tcgatatcgg caccgtcaag ctcatccggg agggccgcat cgagatacgc
     8581 cgcggcctac gttccatcag ccaagacgca gtcaccttcg atgacggctc gacgcgccgc
     8641 tgcgatgcca tcgtccttgc cacgggcttt cgcgccggct tcgaccgttt cctcaaccag
     8701 ggccgcctcg cggcgccggc aggactgcat ttctgcggct ttcgcgtctc cccgaccggc
     8761 atgctgcgcg atatcggcct ggaggcccgt cagatcgcgg aacgcatctc aactcattga
     8821 tctttcaggg actgctggaa actcgccaaa aaggcatacc cattttcgcg gcctacgcgc
     8881 tctaatgttc cagggaagat tcaacctcgg cttggatttg cgagcgtatg cctgacaatc
     8941 gcagcttcgt cgcgaacatt gcggcccgct atgggccccg gctgcgccgc tttttgtcgg
     9001 tgcggctgcg caatgccgcg gacgtaccgg acctggccca agaggtgttc ttgcgcctct
     9061 tgcgggtcga tggctccgag agcatccgca gtccggaagc ctatctgttc accattgcca
     9121 gccatgtgat tcaccagcac gcggtgcgcc gttcgagcga gcccgtctcg gtggatatcg
     9181 ccgaagtatt ctccgaactc agaaccccct cgagcgatga cccgcccgac caggtcgccc
     9241 acgcccagcg gctcgacgaa ctcgaacgcg tgctcgccta cctgcccgca cgcgtcgcga
     9301 cggccctcgt tctacatcgg gtcgccggtt attctgtgca ggaggtgggg gacgaactcg
     9361 gcgtatcgcg tgagaccgcc aagaaatatc tggcgcgcgc ggtcgagcac tgtagaaatc
     9421 ttcgagcctc cgaaggggcc accagactat gagaatcgga ccagcaagca gcaagactaa
     9481 cccggtgatc gtgaccgagg cagcggcatg gtttatcgag ttccgcgccg gcgatgtcaa
     9541 cggcgaggcg cgcctgcgct tcatcgactg gttgcggcgc tcgcccgaac acattcaggc
     9601 gtacctcgag atatcgggcg tctgggcgga gctgccgacg gcggatcccg aaggcaagct
     9661 cgacattgcc gcgctcatcg cccgggcccg caacgaagca gacgtcatcg cgctgcccgc
     9721 gctgcgcccc tcgatcgcca acctcccgct gcatgagaat tcgccgacct tctggcaacg
     9781 tccgcggcgc gctttgctcg ccatggcggc gctcgggctg ctggctgcgg tgctgctgcg
     9841 gtatgtgggc aacgatctta ccgactccta tgccaccggg atcggcgaac agcgcaccat
     9901 ccagctcagc gacggatcga cggtggaatt gaatgcacgc tcccgtgttc aggtgcaact
     9961 cacgaaccgt cagcgcaatg tgtcgctgct cgaaggccag gccttgtttc gcgtcgccaa
    10021 ggacaagcag cggccttttg tcgtgagcgc cggggatgcc caagtgcgcg cggtcggcac
    10081 tgagttcgac gtgtatcgca aacaagtctc gaccgttgtc acagtcgtcg aaggtcgcgt
    10141 tgaaaccttc gacccctcgg ccagcgccgg tgcggcgccg atcgttctat ccgccggcga
    10201 gcagctcacc gtcctgccgc ataccgtgac caagccgacc aagacggata ccttggtcgc
    10261 caccgcatgg gtacaacggc gtctcatgtt cgaggaaacg ccgttgagcg aggtcgcgga
    10321 agaattcaat cgctacaacc gccggccgtt gaccatcgat gatcgcgatc tgcagaaaat
    10381 aaaaatcagc ggcgtctatt cctcgaccga tccggcatcg ctcatcaatt ttctgcgcag
    10441 ccagggctcg attcaagtta tcgaaacgga gaatcaagtg cgcgtcgtcc gccgagcgcg
    10501 ctaaccatac ccatttttta tgtgacaacg ctctactgtc atcagacctg gcggcttgcg
    10561 ccggccggca tcaagaacct ctcaagaggc gcgggagcgt ttatgcgtat tgcagttgtc
    10621 acggcggccg tgtgcctgtc catcgtcggt ctatccctgg ctgacggcgc gaatgccgcc
    10681 attcgaaaac ccacccacat cgcggcgcaa ggtctcgggc ctgcgctcaa gcaactcgcc
    10741 cagagccgcg gattgcaggt gctgtacttg tcgagcacgg ttcgcgatct gcgtacccgt
    10801 ggcgcgaacg gcgacatcac cgccgacgaa gcgttcgacc agttgctcac gggcaccggc
    10861 cttacctacc gctatctcga cgagaacacc gtgaccgtcg tgccgctgag cgcgccggcg
    10921 ctgcccgacg gggccggcgt gctgctgcag gcgccggacg gcgatgctgt cgccacggca
    10981 gccgcaatct ccgatgtgac gaatgtttcg gccgacaagt atctgctggc gcaaaatgtt
    11041 ccggccagcg cgcaagcatc gagcgcaacc gaagcggaat ctccacccct gcaagagatc
    11101 atcgtcaccg gctcgcgcat cgcttcgccg aatgaagtga gcaccagtcc cattcaggtg
    11161 atctcttcga aatccatcca ggtgtccggc aagaccgaca tcaccgacat catcagccag
    11221 ctgccgcaga ttttcaccaa cgatttgggc caagacctgg gcaacggcac ctcggggctg
    11281 accaccgccg gcggcgtcgc caccgcggac ctgcgcggcc tcgggcctgg ccgcacgctg
    11341 gtgctggtcg atggccgccg cttgggcgtc ggctcgccga acacggccat agcgcagccc
    11401 gctccggatc tcgatcaaat accggcgggc ctcgtggaac gcgtggaagt cgtcaccggc
    11461 ggtgcctccg cggcctacgg ctcggacgcc atcgcgggcg tcgtgaactt catcatgaag
    11521 cgcaacttcg agggattcca agtcgacggt caagtgggcg agaactatca cgacaacaac
    11581 gatgccttca tgcagaacct ggtgcgtcag ttcggctaca cgccgcccac cggcaccgaa
    11641 caagacgggc gcaaccgcac cttcgatgtg ttgatgggca cgaacttcgc ggacggcaac
    11701 ggcaatgtca cggggtactt gagctaccgc cacgccgatc cggtcgtcgc cagtcaacgc
    11761 gattttggcg cctgccagtt gttcccgacc tacgacaacg ccggcaatgt caccggcgtc
    11821 acctgcggcg gctcgagcaa ttcgaactgg ttcgagccga c
//
LOCUS       DASWZT010000541         2446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_62403, whole genome shotgun sequence.
ACCESSION   DASWZT010000541 DASWZT010000000
VERSION     DASWZT010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62403"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="VGE92_15455"
     CDS             complement(<1..1434)
                     /locus_tag="VGE92_15455"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY1315277.1"
                     /translation="MSQSLAEQFATTALSGGNAGYIEDLYEQFLEDPSRIDPTWAAYF
                     RRLQGGASGEVAHAPIRERLLASLKSAPRPASAAPSAEAGGASAKQGAVSRLIQVYAN
                     RGHLVANLDPLGLQERAKPYVLNPEYFGLSDADLNTEFFTGSRNAAIAERAKLKDILA
                     DLKFVYTDTIGAEFAHVSNTDERLWLQDSFQVARMQRRFSAAEKKNILWQLTAAEGLE
                     RYLHTKYVGQKRFSLEGGDSLIPLLDELVQQAGKASVEEAIVGMAHRGRLNVLVNLLG
                     KSPKDLFNEFEDHYDLAKLRGSGDVKYHKGFSADLKTESGNVHVVLAFNPSHLEVVNP
                     VVEGSVRARQERRGDIMGDKVLPVQIHGDAAFAGQGVIMETLQLSQARGFYTGGTVHV
                     IVNNQVGFTMSDPRDARSTLYCSDVAKMVEAPIFHVNADDPEAVCFVARFALEYRMKF
                     HKDVVIDLVCYRRLGHNEADEPAATQPL"
     gene            complement(1555..2040)
                     /locus_tag="VGE92_15460"
     CDS             complement(1555..2040)
                     /locus_tag="VGE92_15460"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HEY1315278.1"
                     /translation="MNRPGLTLARERCAADRRRRVWWSLCYGNFNPRRRMPPRRGLER
                     HRFYFLDWHSSHLLAVAIGVLLLSAMDAFLTLLLLERGAAEVNPIMAALLYRSVAAFA
                     ALKMAMTSVSVVLMVFLSRYRFMRLLRVEWVLCAVLVAYGSLICYEVSLLKNLLELPI
                     F"
BASE COUNT          489 a          766 c          736 g          455 t
ORIGIN      
        1 gagcggttgc gtggcggcag gttcgtccgc ctcgttgtga cccagccggc gatagcagac
       61 gaggtcgatt accacgtcct tgtgaaattt catccggtat tcgagcgcaa agcgcgcgac
      121 gaaacacaca gcctccggat catcggcatt gacgtggaaa atgggtgctt cgaccatctt
      181 cgccacgtcc gaacaataca gcgtcgagcg cgcatcgcgc ggatcgctca tcgtaaagcc
      241 gacttgattg ttgacgatga catgcaccgt gccgccggtg tagaagccgc gcgcctgcga
      301 cagctgcagg gtttccatga tgacgccctg gccggcaaac gccgcatcgc cgtgaatttg
      361 caccggcaac accttgtcgc ccatgatgtc gccgcgccgt tcctggcgcg cgcgaaccga
      421 tccctccacc actgggttca cgacctcgag gtgcgacgga ttgaacgcca acaccacgtg
      481 cacgttgccc gactcggttt tcagatcggc tgaaaacccc ttgtgatact tgacgtcgcc
      541 ggagccacgc agcttcgcca gatcgtagtg atcttcgaac tcgttgaaca aatccttcgg
      601 cgacttgccg agcaggttca ccagcacatt gaggcggccg cggtgcgcca tgccgacgat
      661 ggcctcctcg acgctcgcct tgccagcctg ttgcaccagt tcgtcgagca gaggaatcag
      721 actgtcgccg ccctcgagcg agaagcgctt ctgacccacg tacttggtgt gcaggtagcg
      781 ctccaagcct tccgccgcgg tcagctgcca gaggatgttc ttcttctcgg cggcgctgaa
      841 gcggcgctgc atgcgcgcca cctggaagct gtcttgcagc cacagccgtt catcggtatt
      901 cgatacatgc gcaaactccg cgccgatcgt atcggtatag acaaacttaa ggtccgcgag
      961 aatgtccttg agcttggcgc gttcggcaat cgcagcgttg cggctgccgg taaagaactc
     1021 ggtattcaga tcggcgtcgg aaagaccgaa gtactccggg ttgagcacgt acggcttggc
     1081 gcgttcctgc aaaccgagcg ggtccaaatt ggcgaccaag tggccgcggt tcgcgtacac
     1141 ctgaatgagc cgcgacaccg cgccttgctt ggcactcgct ccgcctgcct cggcgctggg
     1201 agcggcgctt gcggggcgcg gcgccgattt gaggcttgcc agcagacgct cgcgaatcgg
     1261 cgcatgggcg acttcgccac tggcgccgcc ctgcaggcgg cgaaagtagg cggcccaagt
     1321 ggggtcgatc cgactcggat cctccagaaa ctgctcgtac agatcttcga tgtagccggc
     1381 attaccgccg gaaagtgccg tcgtggcgaa ctgctcggcg agggattgac tcatggtgag
     1441 ggcggtgaaa atctcaagta catcaatatg atgggttgcg cgggcgggtt tcctgactta
     1501 tctacagttt agcccaaggc ggcgcaatat ggaatgtgac ccagtccgcc aaaactagaa
     1561 gatcggcagc tccaataggt tcttgagcag cgacacttcg tagcaaatga gactgccgta
     1621 agcgaccaac actgcgcaca gcacccactc gacccgcagc agtcgcatga atcgatagcg
     1681 cgacagaaac accatgagca cgacgctgac gctggtcatg gccattttca gcgcggcgaa
     1741 tgccgcgacg ctgcgataga gcagtgccgc catgatgggg ttcacctcgg cggcgccgcg
     1801 ttccagcagc agcaaggtca gaaatgcgtc catcgcgctc aataacaaga ccccgatcgc
     1861 aacggccagc agatgcgagg agtgccagtc gagaaagtag aagcggtggc gctcgagtcc
     1921 acgccggggc ggcatgcgac gccgcggatt gaaattgccg taacaaagcg accaccagac
     1981 ccggcggcgc cggtcggcag cgcaccgttc acgcgccaac gtcaatccag ggcgattcat
     2041 tcgatccatc cgccacacta tcccgataca tactccccct gtgcaagctc aatgcgccat
     2101 aacgagcgct ggccgacggt ttgtttcgac caacctctgc atggtttgac ttatgcttcg
     2161 cgaatgcgcg cacaggatct cgggggcgac aaactgatgt cgaaagttca aatcactcgt
     2221 cgacggttcg cggcgggaat cggattgttg gccgcgtccg cgggcagcgc gttcgcgcag
     2281 tcgccgcccc cgaaagttgt acgcgagccg ccacccgccg gctggcctgc caaggagata
     2341 tatccagtat ggccgggtcc tgcaccgggg atcgacgatt tccggccgcc ggttcttgcg
     2401 gctgattggt ctgcggcgtt cctcaggggc gtctccacac ccgccg
//
LOCUS       DASWZT010000542         2331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_14425, whole genome shotgun sequence.
ACCESSION   DASWZT010000542 DASWZT010000000
VERSION     DASWZT010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14425"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="VGE92_15465"
     CDS             <1..1016
                     /locus_tag="VGE92_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315279.1"
                     /translation="ESWPAIERAVGCIGGLLGEDGLLPISVSHEGYLAQPVHAYWDDF
                     WALRGLLDAVDLAKAAGQSASAQRWQDLCTRFAAALYASIETTRAQRKLDFIPGSIEW
                     ADFDPTATANAIYLLDVPDGLNRVAVDRTFDKYLADWRSKRSGAVVAANYTPYEIRII
                     GAFVRLGRRDAALELLRFFLSDRRPVPWNQWPEIAWRDATAPAHLGDLPHTWISAEYV
                     LAVRSMFAYEREADRCLVLAAGIAAEWIEGDGVHVSKMPTLYGSLSYSLRRVDSTTVR
                     FEFAEQIAARLVLRPPLATKLCRVQVDGSVCTSFDADSVTLPRSPTQVICTTLAVPGR
                     TGL"
     gene            1062..>2331
                     /locus_tag="VGE92_15470"
     CDS             1062..>2331
                     /locus_tag="VGE92_15470"
                     /inference="COORDINATES: protein motif:HMM:NF017775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SbmA/BacA-like family transporter"
                     /protein_id="HEY1315280.1"
                     /translation="MTERSKEISGTAEGESAHLGFARQVGLISRTIFGSPVGKTLVLL
                     MIVMILVVAGTAYGQIRLNGWNKPFYDAMSRRDLRDFLYQLGVFFLIAGFLLTLNVIQ
                     RWLVETLELKMREGLVVSLLRDWMLPRRAFWLANAGSMGVNPDQRIHEDARKLCELSA
                     DLGIGLLQAAILFLTFSGVLWGLSKDFVFRVSDADYTVPGFMVWAAILYASAGSLLSY
                     WVGRGLIGRNAQRYAREADLRFSLVRVNEHVDDISLAEGEADEKRRIEMHLNNVLAAT
                     RRIVMGHANLTWVTAGFGWITIVAPILVAAPLYFSGKVSFGGLMMAAGAFSQAQSSLR
                     WFIDNFSAIADWRATLLRVANFRYALAVSHEPRGFGGRITYSEGEAGTITIENLEIVS
                     SANADKLKESNVAVKVGERVLILGAPGTGKT"
BASE COUNT          386 a          687 c          782 g          476 t
ORIGIN      
        1 gcgaatcatg gccggctatc gaaagagcgg tcggatgcat cggcgggctg ctcggcgagg
       61 acggactcct gccgatatcg gtgagtcacg aaggctacct tgcgcagccc gtgcatgcgt
      121 attgggatga cttctgggcg ttgcgcggtt tgctcgatgc ggtggatctg gcgaaagccg
      181 ccggccagtc ggcgtctgcg caaagatggc aggatctgtg cacgcgcttt gcggccgcac
      241 tgtatgcgtc gatcgagacg acgcgagcgc agcgcaagct cgacttcatc cctggcagca
      301 tcgaatgggc cgatttcgat ccgaccgcga cagccaatgc aatttatttg ctggatgtgc
      361 ccgacgggtt gaaccgcgtc gccgtcgacc gcacgttcga caagtatctc gccgactggc
      421 gcagcaagcg cagcggcgcc gtcgtcgcgg caaactatac gccctacgag attcgcatca
      481 tcggcgcgtt cgtgcgcttg ggcagacgcg atgcggcgct cgagttattg cggttctttc
      541 tatccgatcg gcgtccggtg ccgtggaatc agtggcccga aatcgcatgg cgcgacgcga
      601 cggcgcctgc acacctcggt gatttgccgc acacgtggat ctccgccgag tacgtgctcg
      661 ccgtgcgcag catgttcgcc tacgagcgcg aagccgatcg atgcttggtg ctggcggcgg
      721 gcatcgccgc ggaatggatc gaaggcgacg gagtgcacgt cagcaagatg ccgacgctct
      781 acggttcgct gagttattct ctgcgccggg tcgattcgac caccgtgcgc ttcgaattcg
      841 cggagcaaat tgcagcccgc ctagtgttgc gtccgccgct cgcaaccaag ctgtgccgcg
      901 tgcaggtcga cggctctgtg tgcacgagtt tcgatgcgga ttccgtgacc ctgccgcgca
      961 gcccgacgca ggtcatctgc acgacactgg ccgtccccgg ccgcacgggc ctctgaggtg
     1021 gggagccggc cgtcgagtgg ggtggtatcc tgcagggcac ggtgacagaa cgctccaaag
     1081 aaatctcggg tactgccgag ggcgagagcg cccatttggg gtttgcacgc caggtcggcc
     1141 tcattagccg cacgatcttc ggctcgccgg tgggcaagac gctcgtcttg ctcatgatcg
     1201 tgatgatcct ggtggtcgcc ggcacggcgt atgggcagat ccggctcaac ggctggaaca
     1261 agcctttcta cgatgccatg tcgcggcgcg acctgcgcga ctttctctac cagctgggcg
     1321 tgttttttct aatcgcgggg tttctgctca ctctcaatgt catccagcgg tggctggtgg
     1381 agactctcga actgaagatg cgcgaggggc tggtggtgag tctgctgcgc gattggatgc
     1441 tgccgcggcg ggcgttttgg ctggcgaatg cggggtcgat gggcgtcaac ccggatcagc
     1501 gcattcacga ggacgcgcgc aaactttgcg agctgtccgc ggatctcggc atcggcctgt
     1561 tgcaggccgc cattctcttc ctaacttttt ccggcgtgct gtggggcctg tcgaaagatt
     1621 tcgtgttccg tgtcagcgat gccgactaca cggtccctgg cttcatggtg tgggcggcaa
     1681 tcctctatgc gagcgcggga tcgctcttga gctattgggt agggcgaggt ctgatcggcc
     1741 gcaatgcgca gcgttacgcg cgcgaggcgg atctgcgctt ctcgctggtc agggtcaacg
     1801 aacatgtcga tgacatctcg ttggcggagg gcgaggcgga cgaaaaacgc cgcatcgaaa
     1861 tgcacttgaa caatgtgctg gcggcgacgc gccgcatcgt catgggccat gccaatctca
     1921 cctgggtcac cgccggcttc ggctggataa ccattgtcgc gccgattctg gtcgcggcgc
     1981 cgctgtattt cagcggcaag gtctccttcg gcgggctgat gatggccgcc ggtgcgttca
     2041 gccaggcgca atcctcgctg cgctggttta tcgataattt cagcgcgatt gccgactggc
     2101 gcgcaacgct gctgcgagtc gcgaacttcc gttatgcgct ggccgtgtcg catgagccgc
     2161 gcggattcgg cggccgcatc acctacagcg aaggtgaggc cggcaccatc accattgaaa
     2221 acctcgagat cgtgtcgtcg gcaaacgcgg acaagctcaa ggaatcgaat gtggcggtga
     2281 aggtcggcga gcgcgtgttg attctcgggg cgcccgggac gggtaagacg c
//
LOCUS       DASWZT010000543         2807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_48015, whole genome shotgun sequence.
ACCESSION   DASWZT010000543 DASWZT010000000
VERSION     DASWZT010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48015"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VGE92_15475"
     CDS             <1..606
                     /locus_tag="VGE92_15475"
                     /inference="COORDINATES: protein motif:HMM:NF040452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6515 family protein"
                     /protein_id="HEY1315281.1"
                     /translation="GVGRPAYDGRGQVLDSRYNHGRYYPPMGTVRASLPEGYRPYFRG
                     GSRYYFSGGIWYAPRGPGFVVIAPPPGLVISVLPPYYSTVWFGGVPFYYADNVYYSWQ
                     PDQNGYAVVDPPPNADAPSPAPAPPPSNGQADLMIYPKNGQSKEQQAADQFECHNWAK
                     GQTGFDPTKPGGGVSGDPEAGRNSYNRAMSACLQGRGYQVN"
     gene            618..875
                     /locus_tag="VGE92_15480"
     CDS             618..875
                     /locus_tag="VGE92_15480"
                     /inference="COORDINATES: protein motif:HMM:NF039524.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315282.1"
                     /translation="MLTAPPSSVVQKRDLHVGGLGANAVGALAAVTDPAGEAAQTWLR
                     RTRDAVRDADDFVHDNPWAALGIVAVLGVAAGYLLAQRRAD"
     gene            complement(879..1730)
                     /gene="fdhD"
                     /locus_tag="VGE92_15485"
     CDS             complement(879..1730)
                     /gene="fdhD"
                     /locus_tag="VGE92_15485"
                     /inference="COORDINATES: protein motif:HMM:NF014671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEY1315283.1"
                     /translation="MSTAPPRLSGTLNVRILRSTADSQEERDDRVAVEAPLEFLLHHP
                     SLGMEPMSFGTTMRTPGEDEFLAAGLLYGEGIVNQAADIDALESSTRRPNVVNVRLRP
                     TVQIVPNTPARLFSAGSSCGVCGTTGLDAAIARAAATRIAATGDVPLSLLLHLPQRMR
                     AAQSKFGDTGGVHASALFDFSGTLLNVAEDVGRHNAFDKLVGMSLISGALPLTERIVV
                     LSGRASFELVQKAIRAGVSILAAIGAPSSLAVNLASTSGLTLVGFLREAHCNVYSHAE
                     RLSRAPG"
     gene            1805..>2807
                     /locus_tag="VGE92_15490"
     CDS             1805..>2807
                     /locus_tag="VGE92_15490"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY1315284.1"
                     /translation="MSKSSDLKTRKAAAVPAGVGTRGIYVAKAENSELWDVDGRRFVD
                     FAAGIAVLNTGHRHPRVMEAVAQQAQEFTHTCFHVAPYESYVRLAERLNALAPGDFAK
                     KTMFLSSGAEAVENAVKIARYHTKRSAVIAFSGGFHGRTLLTMALTGKVMPYKRGFGP
                     FPAEVYHAEFPQAFRGITTEQALADLERLFHGDVDPKSVAAIIIEPVQGEGGFNVAPF
                     DFLRALRRLCDEHGIVMIADEVQSGMARTGKMFCIEHSGVVPDLITTAKGLGGGFPLS
                     GVTGRASIMDSAHAGGLGGTYGGNPISIAAAHAVLDVIEAEGLCNRAVSVGDKMRSRL
                     "
BASE COUNT          501 a          883 c          923 g          500 t
ORIGIN      
        1 ggtgtgggac gcccggccta tgacgggcgc gggcaggtct tggacagccg ctataaccat
       61 ggccgctact acccgccgat gggtaccgtc agggcgtcgt tgccggaggg ttatcgaccc
      121 tacttcaggg gcggcagtcg ctactatttc agcggcggca tttggtatgc gccgcgcggc
      181 ccggggttcg tcgtgatcgc accgcccccg ggcctggtga tctcggtgct gccgccctat
      241 tactcgaccg tctggtttgg cggagtcccc ttctactatg cggacaatgt gtactactcc
      301 tggcagcctg atcagaacgg ctatgccgtg gtggacccgc cgccgaacgc cgatgcgccc
      361 tcgcctgcgc ccgcgccgcc gcccagcaac gggcaagccg acctcatgat ttatccgaag
      421 aacggtcagt ccaaagagca gcaagccgcg gaccaattcg agtgtcacaa ttgggccaag
      481 ggccaaaccg gattcgatcc gaccaagccg ggcggcggtg tgtcgggcga tcccgaagca
      541 ggccgcaaca gctacaaccg tgccatgtcc gcctgcctgc agggccgtgg ctaccaggtc
      601 aactagggag aattcacatg ttgacggcgc ctccaagttc ggtcgtgcag aagagagatt
      661 tgcatgtggg cggtctcggt gcgaacgccg taggcgcgct cgccgcggtg acggatccgg
      721 cgggcgaggc ggcgcaaacc tggctcaggc gaactcggga tgccgttcga gatgcggacg
      781 acttcgtcca tgacaacccg tgggctgcgc tcggcatcgt cgccgtgctc ggagtcgcgg
      841 caggatatct gttggcgcag cgccgcgcgg attgaggttt accccggcgc gcggctcagg
      901 cgctcggcgt gcgagtagac gttgcagtgc gcctcgcgca agaatcccac caaggtcagt
      961 cccgaggtcg atgcgaggtt gaccgctaaa ctggatggcg cgccgatggc agcgagaatg
     1021 ctcacgccgg cacggatcgc cttctgcact agttcgaaac tggcgcgccc gctcaagacc
     1081 acgatgcgct cggtcagcgg caaggcgccg gatatcaaac tcatgccgac cagcttgtcg
     1141 aaggcattgt ggcggcccac gtcctccgcc acgttgagca aagtgcccga aaaatcgaac
     1201 agcgccgacg catgtacgcc gccggtatcg ccgaacttcg attgcgccgc acgcatgcgc
     1261 tgcggcagat gcagcagcag cgataaaggc acgtctccgg tcgccgcaat ccgcgtcgcg
     1321 gcggcacggg cgatcgccgc atcgagtccc gtggtgccgc aaacgccgca actcgagccg
     1381 gcggaaaaca gccgcgccgg ggtgttgggc acgatttgca ccgtcgggcg caggcgtacg
     1441 ttgacgacat tcggccgccg cgtgctcgac tcgagtgcgt cgatgtctgc cgcttgattc
     1501 acgatgcctt ctccataaag gagtcccgcg gcgagaaatt cgtcctcacc cggggtgcgc
     1561 atggtcgtgc cgaaggacat gggttccatc ccgagcgagg ggtgatgcaa caggaattcg
     1621 agcggcgctt ccacggcgac ccgatcgtct cgctcctcct ggctgtcggc ggtggagcgc
     1681 agaatccgca cattgagagt cccgctcaac ctgggaggcg ccgtgctcat gattcaacct
     1741 tttgctcaaa ttagcggcca atgataccgt tcccggtcta tgcggcgcat ccaggggagc
     1801 gtaaatgtcg aaatcatccg atcttaaaac tcgcaaggcg gccgcggtgc cggccggcgt
     1861 cggtacccgg gggatttacg tggcgaaggc ggagaactcc gagctatggg atgtcgacgg
     1921 ccgtcggttc gtcgattttg cggcgggaat tgccgtcctc aataccgggc accgtcatcc
     1981 gcgggtcatg gaggcggtgg cgcagcaggc gcaagaattc acccacacct gcttccacgt
     2041 tgcgccctac gagtcctacg ttcggctcgc cgagcggctc aatgcgttgg cgcccggcga
     2101 tttcgccaag aagacgatgt ttttgagttc gggtgccgag gccgtcgaga acgccgtcaa
     2161 gatcgcgcgc taccacacca agcgttccgc ggtgatcgct ttttccggcg gcttccatgg
     2221 ccgcacgctc ttgaccatgg cgctgaccgg caaggtcatg ccgtacaagc gcggcttcgg
     2281 gccgttcccg gccgaggtgt atcacgccga atttccgcaa gcctttcggg gcattacgac
     2341 ggaacaggcg ctggccgatt tggagcggct gtttcacggc gacgtcgatc cgaagtcggt
     2401 cgccgccatc atcatcgaac cggtgcaagg cgagggcggc ttcaacgtgg cacccttcga
     2461 tttcctgcgc gccctgcgcc gcttgtgcga cgagcacggc atcgtgatga tcgccgacga
     2521 agtgcagagc ggcatggcgc gtaccggcaa gatgttctgc atcgaacatt cgggcgtggt
     2581 gcccgacctc atcaccaccg ccaagggttt gggcggaggc ttcccgctat cgggagtgac
     2641 gggacgtgcc agcatcatgg atagcgcgca tgccggcggc ctcggcggca cctacggcgg
     2701 caatccgatc agcatcgcgg ccgcgcacgc agtattggat gtgatcgaag cagagggact
     2761 ttgcaatcgg gcggtgagcg tcggcgacaa aatgcgcagc cggcttg
//
LOCUS       DASWZT010000544         4656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_38381, whole genome shotgun sequence.
ACCESSION   DASWZT010000544 DASWZT010000000
VERSION     DASWZT010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38381"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="VGE92_15495"
     CDS             <1..707
                     /locus_tag="VGE92_15495"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1315285.1"
                     /translation="VSLSANAGEFIAVVGANGAGKSTLLATLAGLLHADAGRIELNGT
                     SIAQLSGTMLARTRAYLPQNARCEWPISVERLVALGLTPTLPVFGGFTADDQLKITRA
                     LETCDLLAQREQPATSLSGGELARAMLARAFVSDPAVLIVDEPVAGLDPSHALRAMRR
                     LHSFATDGRLVIASMHDLTLAARYATRIIALRHGRVECDGPMDKLTPDLIRAVFDIEA
                     RVLGLAARTYIDYLDP"
     gene            complement(726..1826)
                     /locus_tag="VGE92_15500"
     CDS             complement(726..1826)
                     /locus_tag="VGE92_15500"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY1315286.1"
                     /translation="MQTPPIPKTEEGAAASVDKSRFKFQKLLQPFTIERGIATPIIWF
                     LLDWLVLGVSIASLLFVEAWWVKVFASLVAALWIARLFVIGHDACHGSYTPNKTLNKW
                     IGRIAFLPSLTPYSLWEIGHNLAHHGFTNLKGRDYVWTPYSPSEFARLPWLRRKLEHV
                     YRSGVGQGVYYMIEMWWKKLFFPGRAHVTTRRVSYTWDSLLVAAFGLLWVGGLVIWGV
                     YSGKSIWSLLVFGFVVPFFLWNCLMGFVIYVQHTHPRVAWYERRDEWSANAGYATTTV
                     HIKLMRPLDGLIHYILEHGAHHVNMGIPLYRLKEAQARLTATLDERLNSEVFTWRYYW
                     NTVRRCKLYDFAQHAWMDFDGRVTSEPLVQTR"
     gene            complement(1921..2460)
                     /locus_tag="VGE92_15505"
     CDS             complement(1921..2460)
                     /locus_tag="VGE92_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315287.1"
                     /translation="MSKSPNARRIGGPLSWEQLARPDTEQCQRFLAESLSLLLADILR
                     SVLSDASPALAADESAVERYRNLADSLADGESRRRTSLGITRAGLAKRCVTWLKLLLT
                     PAPNAPSSAPMREFVDALGLFPALFDSEPSARSRRARLTWLLTHDRRLDASTREAATA
                     LIEACEHLGVLLWPDAETA"
     gene            2647..3456
                     /locus_tag="VGE92_15510"
     CDS             2647..3456
                     /locus_tag="VGE92_15510"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY1315288.1"
                     /translation="MSLAAGVRRIVAGNAGLMTGPGTNTYLFGSREVAVLDPGPDDER
                     HFDAIMSAAAGAVRWIVVTHTHKDHSPLAKRLAAHTGALVIGLPAPGDGRQDSTFESK
                     YTPADGERLAVGEVPLVAIHTPGHASNCVCYLLEHERMLFTGDHVLEGVSPVILPPDG
                     DMDAYLHSLEKLRAYDFERIAPGHGGVMERGKEVLRALRLHRLAREEKVVQSLRRLGR
                     ATLDELTAAVYDDVALERHRWARLTLEAHLIKLVRERRVGDSDGIWRLRDT"
     gene            3456..>4656
                     /locus_tag="VGE92_15515"
     CDS             3456..>4656
                     /locus_tag="VGE92_15515"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1315289.1"
                     /translation="MRGLTVLLHAASVRRGEKWALREVSWQLKPGERWALLGDNGSGK
                     TQLLKLLSGAVWPTPGREPEIPARSYRLGQRAVDLIEAKRRIAYVGAEQQDKYARYGW
                     NLRVHEVVATGLHRTDLLLMPVTAREARQVAAKLQDCGILQHAQRRFLSLSYGQKRLV
                     LLARALAQDPDWLLLDEFYNGLDTHFRRRLDAVLAAARRGGQSWVASAHRASDVPRGT
                     GRMLELSGGRVSAVKLMRRSEMLDLARRAGENRPVSRGTPRIDGNAPLLMRLSKVNLY
                     VEYRLVLRDLDWQLRGGEHWAVFGANGAGKSSFLKLLYGDLSPALGGHIERSGFPPGT
                     SIAQWKRQTGFVSPELQSDYCVDVSVADLVASGRYASIGLVDAATPEDRRAAAFWLRF
                     FGLLSVAK"
BASE COUNT          862 a         1428 c         1512 g          854 t
ORIGIN      
        1 gcgtatcctt gagcgcgaat gccggcgaat tcatcgcggt cgtcggtgcc aacggggccg
       61 gtaagtccac cttgcttgcg acgcttgcgg gactgctgca cgccgacgcc gggcggatcg
      121 aactcaacgg aacatcgatc gcacagttgt cgggtacgat gctggcccga acccgcgcct
      181 acctgccgca aaacgcgcgc tgcgagtggc ctatttcggt agagcgcctg gtcgcgctcg
      241 ggctcacgcc cacgctgccg gtattcggcg ggttcacggc cgacgatcag ctgaaaatca
      301 cacgtgcgtt ggagacctgt gacctgttgg cgcagcgcga acagcccgca acttcgctct
      361 cgggcggcga actggctcgc gcaatgctgg cccgggcgtt cgtcagcgac cccgcggtgc
      421 tcatcgtcga tgaaccggtt gcaggcttgg atcccagcca tgcgcttcgt gccatgcgcc
      481 gtttgcactc gttcgcgacc gacggcaggc tcgtcatcgc ctccatgcac gatctgacct
      541 tggccgcgcg ctatgcgacg cgaatcattg ccttgcgcca cggtcgtgtt gaatgcgacg
      601 gcccgatgga taagctgacg ccggacttga tacgcgcggt tttcgacatc gaggcacgcg
      661 tgctgggatt ggcagcacga acttacatcg actacctaga tccgtagcgg cgcctcgcgg
      721 cagcgctagc gagtctgaac cagcggctcg gaggtgacgc gtccgtcgaa gtccatccag
      781 gcgtgctgcg cgaagtcgta cagcttgcag cgccgcacgg tattccagta gtaacgccag
      841 gtaaacactt cggagttgag ccgctcgtcg agcgtcgctg tgagccgcgc ttgagcctct
      901 ttgaggcgat acagcgggat acccatgttc acgtgatgcg cgccgtgttc gagaatgtag
      961 tgaatcaagc catcgagcgg gcgcatcagt ttgatatgta cggtggtggt cgcgtagccg
     1021 gcgtttgccg accattcatc gcgccgctcg taccacgcga ctcgcgggtg agtgtgctgc
     1081 acgtagatca cgaagcccat caaacaattc cacaggaaga acggtactac aaagccgaaa
     1141 accagcagcg accaaatgga cttgccgctg tacacgcccc atatcacgag acccccgacc
     1201 cacagcaggc cgaaggccgc caccaataag ctgtcccagg tgtagctgac gcggcgggtc
     1261 gtgacgtgcg cgcgaccggg gaaaaaaagt ttcttccacc acatctcgat catgtagtac
     1321 acgccttgcc cgacaccgct gcgatacacg tgctcgagct tgcggcgaag ccacggcagg
     1381 cgcgcaaatt cggagggcga gtagggcgtc cagacgtaat cgcggccttt caaattggtg
     1441 aaaccgtgat gcgcgaggtt atggccgatt tcccacaggc tatacggcgt cagcgacggc
     1501 aggaatgcga ttctgccgat ccatttattg agcgtcttat tcggcgtata tgagccgtgg
     1561 caggcatcat gaccgatgac gaacaggcgg gcgatccaca atgccgccac cagcgaggcg
     1621 aaaaccttga cccaccacgc ttcgacgaac agcaagctcg cgatcgacac gccgagtacc
     1681 agccaatcga gcaagaacca gatgatcggc gtggcgatac cgcgctcgat cgtgaacggc
     1741 tgcagcagct tttgaaactt gaagcgcgac ttgtctaccg acgccgcagc gccttcctca
     1801 gtcttcggta tgggaggtgt ttgcattcaa gactccggcc tttacttaac ccaagggccc
     1861 ccgacggcga cttaatataa tacgcctctt caggctatcg gagcggaaat gcgtccggtt
     1921 ctaggcggtc tctgcatcgg gccacagcag cacccctaag tgctcgcaag cctcgattaa
     1981 ggcggtggcg gcctcccggg tacttgcgtc caagcgccgg tcgtgggtga gtagccaggt
     2041 cagccgggct cggcgcgagc gggccgatgg ttcactgtcg aaaagggccg gaaacaggcc
     2101 cagggcatcg acgaattcgc gcatcggcgc gctggacggg gcgttcggcg cgggcgtcaa
     2161 gagcaacttg agccacgtga cacagcgttt ggcgagccct gcgcgtgtga ttccgaggct
     2221 ggtgcggcgc cgcgattccc catcggccag cgagtcggcg aggttgcggt agcgctcgac
     2281 cgccgactca tccgccgcca acgccgggct ggcatcagag agcaccgatc gcaatatgtc
     2341 cgcgagcagc agcgacagcg actcggccaa gaagcgttgg cactgttccg tatccgggcg
     2401 tgccaattgt tcccaagaca gaggaccacc aatgcgtctg gcgttcggtg actttgacat
     2461 gtaccacagt gtagcggctc cgctgctcat cgcaactgtg tcaatatgcg ataaacgtca
     2521 ccgacgtggg taatattcgc aaattcgagg tcggctcgcc ggtggagacc cacagcttcg
     2581 aaaaattaat ccaaatcgga ggacacccgc ctggaagcta gatcgcagct cgccgccgag
     2641 aagtgcgtgt cgctggcggc gggtgttcgg cggatcgtcg ccggcaatgc cggattgatg
     2701 accggtcccg gtaccaacac ctatctgttc ggatctcggg aagtggctgt gctcgatccc
     2761 gggcctgacg acgaacggca cttcgacgcg attatgtcgg ctgcggctgg cgccgttcgc
     2821 tggatcgtcg tgacccacac ccataaggac cactcgcctc tcgccaagcg ccttgccgca
     2881 cacacgggcg cgctggtcat tggcttgccg gcgcccggcg atggccggca ggactcgacc
     2941 ttcgaatcca agtacacgcc cgccgacggc gagcgcttgg cagtcggcga ggtgcccctg
     3001 gtcgccatcc atacgccggg gcacgcctcg aattgcgtgt gctacctttt ggagcacgaa
     3061 aggatgcttt tcaccggcga ccatgtgctc gaaggggtgt cgccggtgat cctaccgccg
     3121 gatggcgaca tggacgccta cttgcactcg ctcgagaagc tgcgagccta cgatttcgaa
     3181 cgcatcgccc ccgggcacgg cggcgtgatg gaacgcggca aggaggtact gcgcgccttg
     3241 cggctgcatc ggctggcgcg cgaggaaaag gtcgtgcaga gtctgcgccg attgggccgc
     3301 gcgacgctcg atgagctgac cgcggcggtt tacgacgacg tggcgctcga acggcatcgc
     3361 tgggcgcgcc tcacgctcga ggcgcatttg atcaagctgg tgcgcgagcg ccgcgtgggc
     3421 gattccgacg gtatttggcg gctccgcgac acctgatgcg cgggcttacg gtattgctgc
     3481 acgcggcgag cgtgcggcgc ggcgagaaat gggcgttgcg cgaggtctcg tggcaattga
     3541 agcccgggga acgctgggct ttgctcggcg acaacggttc gggcaaaact caattgttga
     3601 agctgttgag cggcgctgtc tggccgacgc ccggccggga gccggaaatc ccggcgcgca
     3661 gctaccgtct cggccaacgc gccgttgatc tcatcgaagc caagcgccgc attgcctatg
     3721 tgggcgccga acagcaggac aaatatgccc gctacggttg gaatctgcgc gtgcacgagg
     3781 tggtggcgac gggtctgcat cgcaccgatc ttctgctcat gcccgtcacg gcgcgagagg
     3841 ccaggcaggt agccgcgaag ctgcaggact gcggcatatt gcaacatgcg cagcggcgat
     3901 tcctgtcgct gtcctacgga caaaaacggc tggtcttgct ggcgcgcgcg ctggcacagg
     3961 atccggactg gttgttgctt gacgaattct ataacggtct cgacacgcac tttcgccggc
     4021 gtctcgacgc ggtgctcgcc gcggcgcgcc gcggcggcca atcatgggtg gcgagtgcgc
     4081 accgggcgag cgatgtaccg cgcggcaccg gccgcatgct cgaactgagc ggcggccgcg
     4141 tcagcgccgt caaactcatg cggcgctcgg agatgttgga tctcgcgcgg cgcgccggcg
     4201 aaaatcggcc tgtgtcgcgg ggtacgccgc gcattgacgg caacgcgccg ctgctcatgc
     4261 ggctgtccaa agtaaacctg tacgtcgagt atcgattggt gctgcgagat cttgattggc
     4321 aattgcgcgg cggcgagcac tgggcggtgt tcggcgcgaa cggtgccggc aagtccagtt
     4381 ttctcaagct gctgtacggt gatctatcgc ccgccctcgg cggacacatc gagcgcagcg
     4441 gctttccccc gggtacatcc atcgcgcaat ggaagcgaca aacgggcttt gtctcgccgg
     4501 agttgcaaag cgattactgc gtcgatgtga gcgtggctga cttggtggcg agcggacgct
     4561 acgcgagcat cggactggtc gatgcggcga cgcccgaaga tcggcgtgcg gcggcctttt
     4621 ggctgagatt tttcgggctt ttgtcggtcg cgaagc
//
LOCUS       DASWZT010000545         3705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_81983, whole genome shotgun sequence.
ACCESSION   DASWZT010000545 DASWZT010000000
VERSION     DASWZT010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81983"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..843)
                     /locus_tag="VGE92_15520"
     CDS             complement(124..843)
                     /locus_tag="VGE92_15520"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY1315290.1"
                     /translation="MDQLQGGRRRLIMPAPVATLGTGEPEIEVELMAQLGPDTVFMMG
                     DNPVLPKMPDKPRLQDFFRHRFTEFTRNHLLQSAKLALNLGLDERVVLACLLHDIANG
                     ALIRADHGYWGAQLVEPYVSEEVSWAIRQHQALRFFPDPSVGYAYPEAYLRFFGPEYA
                     PPPHIQRAYEEARKHRWYMTSRLITLNDLYAFDPTVTVDLEEFTDIIGRHFRQPAEGL
                     GFDGSAVAHMWRTMIWPNNLL"
     gene            complement(883..1902)
                     /locus_tag="VGE92_15525"
     CDS             complement(883..1902)
                     /locus_tag="VGE92_15525"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY1315291.1"
                     /translation="MIIDCHGHYTTAPAALQKFRDEQIAGLKADVGVPLTAQTRISDD
                     EIRDSLENAQLKLQRARGTDLTIFSPRASAMAHHIGNETTSRRWTQACNDLIYRVVTL
                     YPSNFVGVCQLPQSPGVAPANSAAELERCVSELGFIGCNLNPDPSGGHWSSPPLTDRH
                     WYPLYEKMVELDVPAMVHVSSSCNPNFHATGAHYINADTTAFMQFLTSDLFADFPTLR
                     FIIPHGGGAVPYHWGRYRGLAQDLKRPLLKDYLLKNVFFDTCVYHLPGIELLHKVIPV
                     DNILFGSEMVGAVRGIDPETGQYFDDTKRYVDALALSDADRRKIFEGNARKVFPRLDA
                     RLARV"
     gene            complement(1899..2807)
                     /locus_tag="VGE92_15530"
     CDS             complement(1899..2807)
                     /locus_tag="VGE92_15530"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY1315292.1"
                     /translation="MSQPAKNPLKSCPPPDPHPVKPAWSLPPGACDAHCHVFGPASKF
                     PYSPDRSYTPPDAPVENLRKLHAHLGISRAVIVHASCHGTQMAVTLDAIASSGGSYRG
                     VACVDDAVTEQELRGLHAGGIRGIRFNFVKHLGGVPDLDVFYRLVARIKPLGWHIVLH
                     FDAEDIMMQQDLLARIDVPFVIDHMGRVKAADGLEQRPFQLLLDLFKNNPLAWVKVCG
                     AERVSVGKRPFTDAVPFARALIAIDSRRILWGTDWPHPNITKDMPNDGELVNLFAEIC
                     PDPAQRQRILVDNPTRLYWADTGEGG"
     gene            complement(2804..3523)
                     /locus_tag="VGE92_15535"
     CDS             complement(2804..3523)
                     /locus_tag="VGE92_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HEY1315293.1"
                     /translation="MIGQSAIAVRNVIRADSASVAQLANYGVATIHEAMGRVGLLDAS
                     IRPIYPGAKLCGTALTILAHPGDNWMLHVAAELLKPGDVPVLGTSSANTDGMFGDLLA
                     TSFRARGALGLVIDAGCRDTADLRDMGFPVWSKAVNGKGTVKATVGSVNTPIVCAGAL
                     VNPGDVVVADDDGVVIVPKSHAARVAAAAAAREKKEAANRQRLAAGELGLDLYGMREA
                     LAKAGLKYYEDEAAYLRELQK"
BASE COUNT          735 a         1151 c         1119 g          700 t
ORIGIN      
        1 acacaccctg agccgtgttc ataaccagat tacccgagca aacgctccgc gcagaacctt
       61 acaagagttg cggccatgag cataagcgat tctcggcatc cgacccatcg acagcgcaga
      121 tgctcagagc aggttgttcg gccaaatcat ggtgcgccac atatgcgcga ccgccgagcc
      181 gtcgaagccc aacccctccg ccggttgcct gaagtggcgg ccgatgatgt ccgtgaattc
      241 ctccaggtcc accgtcaccg tgggatcgaa ggcgtacaga tcgttcaacg tgatcagccg
      301 cgaggtcatg taccagcgat gcttgcgggc ctcctcgtag gcgcgctgga tatgcggcgg
      361 cggtgcgtac tccggcccga aaaatcgcaa gtacgcttca gggtaagcgt aaccaaccga
      421 cggatcgggg aaaaaccgca acgcttggtg ttggcgaatc gcccaactga cctcctcgct
      481 gacatacggc tcgaccagtt gcgcgcccca gtagccgtga tccgcgcgga tgagtgcgcc
      541 gttggcaatg tcgtgcaaca ggcacgccag cacgactctt tcatccagcc ccaagttcaa
      601 ggcaagcttt gcgctctgca gaagatgatt gcgcgtgaac tcagtgaatc gatggcgaaa
      661 aaaatcctgc agccggggct tgtccggcat tttcggcaat accggattgt cccccatcat
      721 gaatacggta tccggcccga gctgagccat gagctcgact tcaatctctg gttcaccggt
      781 gccgagcgtc gccacgggag ccggcatgat gagccgccgc cgcccgcctt gtagctgatc
      841 catgtcctgc actcgcctga tgggttcagt ccattataag catcaaacgc gtgccagcct
      901 cgcatccagg cggggaaaca ctttgcgcgc gttgccctcg aatatcttgc ggcggtcggc
      961 atcgctcaga gcaagcgcgt ccacatagcg cttggtatcg tcgaagtact gaccggtctc
     1021 cgggtcgatg ccgcgaaccg cgcccaccat ttccgaaccg aacaaaatat tgtcgacggg
     1081 aatgaccttg tgcagcagtt cgatgcccgg caaatgatag acgcaggtgt cgaagaacac
     1141 gttcttcaac agatagtcct tgagcagagg ccgtttgagg tcttgcgcca gtccgcgata
     1201 tcggccccag tgatagggga ccgcgccgcc gccgtgcgga atgatgaagc gcagggtggg
     1261 aaagtcggca aacaagtcgc tggtcagaaa ttgcatgaat gccgtggtat cggcattgat
     1321 gtagtgcgca cccgtggcgt gaaagttcgg gttgcaggaa gaactgacgt gcaccatggc
     1381 aggcacatcg agttccacca tcttctcgta caacggatac cagtgccgat cggtgagcgg
     1441 cggcgaggac cagtggcccc ccgaaggatc cggattcaga ttgcagccga tgaaccccaa
     1501 ctcgctcacg cagcgctcga gttctgcggc gctgttcgcg ggcgccactc ccggagactg
     1561 cggcaattgg cagacaccga cgaagttgga cggatacagg gtcacgacgc gatagatgag
     1621 atcattgcaa gcctgcgtcc agcggcggct ggtcgtttcg ttgccgatgt ggtgcgccat
     1681 tgcggaggcg cgcggcgaaa aaatggtgag atccgtgccg cgggcgcgct gcagcttgag
     1741 ctgggcgttc tccaggctgt cgcgaatctc atcgtcgctg atgcgcgtct gcgccgtcaa
     1801 cggaacgccg acatcggctt tcaggccggc gatttgctcg tcgcgaaatt tctgcagcgc
     1861 ggcgggcgct gtcgtgtagt gaccgtggca gtcgatgatc atccgccctc ccccgtatcg
     1921 gcccagtaca aacgcgtggg attatccacg agaatccgct gccgttgtgc cgggtccggg
     1981 caaatttccg cgaacagatt caccagctcg ccgtcgttgg gcatgtcttt ggtgatattc
     2041 gggtgcggcc agtccgtgcc ccacaaaatt cgccgcgaat cgatcgcaat caaggcgcgg
     2101 gcgaacggca cggcatcggt aaacggccgc ttgcccaccg atacgcgctc ggcgccgcac
     2161 accttgaccc acgccagcgg gttgttcttg aacaaatcca gcagcagttg gaacgggcgc
     2221 tgttcgaggc cgtcggccgc cttgacgcgc cccatgtggt cgatgacgaa cggcacgtcg
     2281 atccgtgcca acaaatcttg ctgcatcatg atgtcctcgg cgtcgaaatg cagcacgatg
     2341 tgccagccca acggcttgat gcgtgccacc aatcgataga acacgtcgag atccgggacg
     2401 ccgcccaagt gcttgacgaa attaaagcga atgccgcgaa tgccgcccgc atgcaggccg
     2461 cgcagttcct gttcggtcac cgcatcatcg acgcaggcga cgccgcgata gctgccgccg
     2521 ctcgatgcga tggcatccaa ggtcaccgcc atctgcgttc catggcagct ggcgtggacg
     2581 atgaccgccc gcgaaatgcc gaggtgagcg tgcagcttgc gcaagttctc aaccggtgcg
     2641 tcgggtgggg tgtagctgcg gtcgggagag tatgggaatt tgctcgccgg accgaatacg
     2701 tggcaatgcg cgtcgcaagc gccgggcggc aggctccagg cgggcttcac cggatgcgga
     2761 tcgggtggcg gacagctctt cagtggattc ttcgcaggct ggctcatttc tgcaattccc
     2821 gcaggtaggc cgcctcatcc tcgtagtatt ttaggccggc cttggccagg gcttcgcgca
     2881 tcccgtacaa atcgaggccc aattccccgg ctgcgagccg ctggcgattc gccgcctcct
     2941 tcttttctcg cgccgccgcg gccgcggcaa cccgagcggc atgcgatttc ggtacgatca
     3001 cgacgccgtc gtcgtccgcc acgaccacat cgcccggatt caccagggcg cccgcgcaaa
     3061 cgatcggggt attcaccgaa ccgaccgtgg ctttcaccgt gcccttgcca ttgacggcct
     3121 tcgaccagac tgggaaaccc atgtcgcgca gatccgccgt gtcgcggcag ccggcgtcga
     3181 tgaccagtcc aagcgcgccg cgcgcccgga acgaggtggc gaggagatcc ccgaacatgc
     3241 cgtccgtgtt cgctgacgat gtgccgagca ccgggacatc tcccggcttt aggagttcgg
     3301 cggcgacgtg cagcatccaa ttgtcgccgg ggtgcgccaa gatggtgagc gccgtgccgc
     3361 acagcttcgc acccggataa atgggacgaa tgctcgcatc caggagtccc acgcgaccca
     3421 tggcttcgtg aatcgtcgcg acgccgtagt tcgcgagttg cgccacgctg gcggaatcgg
     3481 cgcgaatcac attcctcacc gcaatcgcag attgtccgat cataaagcac ctttctgcaa
     3541 acctcggatg atcacgtata ctatcaagga atgagcgata tacctgctat agcaatttgg
     3601 catgggtcat gaggctaagc gcccgatgga tttgaaacag ctggagtatt ttcgacacgt
     3661 ggccgaactc ggcagcttca cccgcgccgc ctcgtaccta tccgt
//
LOCUS       DASWZT010000546         2651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_52805, whole genome shotgun sequence.
ACCESSION   DASWZT010000546 DASWZT010000000
VERSION     DASWZT010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52805"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..573
                     /locus_tag="VGE92_15540"
     CDS             151..573
                     /locus_tag="VGE92_15540"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY1315294.1"
                     /translation="MNRYLLDTNIISSLAKAAPAAALVAWMADQTDDDLFITALTLGE
                     LRRGILERNSGRKRSQLERWFSGPEGPQSLFANRILPFDEKAALIWSRLMAEGAAAGS
                     ARGALDMIVAAVAEANHCIVVTDNPKHFAGLKTVTPTR"
     gene            complement(579..2480)
                     /locus_tag="VGE92_15545"
     CDS             complement(579..2480)
                     /locus_tag="VGE92_15545"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1315295.1"
                     /translation="MVSQSIDPDEWQSLYASAAAALGSGQLQRAIQLYDSAIHRKPDH
                     AEAYYKRANALNGLGRWELALADYDRAIALNPAYAYAFCNRGTVLERMGRWDEALGSY
                     DRAVALNPGDFLAYYNRASVLKHLKRLEEALSSYSQAIALKGDYFAAYFNRGNILKEL
                     ERYEAAASSYTQALALDPEHKYLLGTRRQSQMQICDWSGLTADLQQLAAGLQAHKAVS
                     APFPLLALLESPILQRLAAQIYVREECPPDDSLGAFPPRRRGDKLRIGYFSADFRHHP
                     VSLLSAQLFETHDRSKFDFTAFSFGPATNDAMRARLVRAFDQFIDVRDRSDADVARLA
                     RDKGIDIAVDLGGFTEHSRTKIFALRAAPVQISYLGYPGTMGAVYMDYLIGDRTVVPQ
                     THQGHYAEKIVYLPHSFLPNDSTREIAATVFARHDLGLPPDAIVFCCFNNNSKIMPST
                     FDSWMRILRRTANGVLWLSQGNAAAANNLRQAAMQRGVDADRLVFAPRMASLPEHLAR
                     YRAADLFLDTLPYNAHATAIDALWAGLPVLTRPGECYAGRVAASLLKTIGLPELIAAS
                     AVQYEDLAVQFAAEPLRLAEVKQKLARNKLKTPLFDTPALTRHLEMAYTRAYERYLAN
                     LAPEHIYLG"
BASE COUNT          547 a          808 c          788 g          508 t
ORIGIN      
        1 caagcggctc gccggcagcg gtgccgatgc gacacgcatc cgcgccaccg tcgcacgcag
       61 catttccggc gatgccggca agaagggcgg catattgaaa gcgctgcgcc gctcgcctct
      121 catcgggacc gatttgggca ggacacggcc atgaaccgct atctcctcga caccaatatc
      181 atcagcagtc tcgcgaaagc cgcgcccgca gcggcgctcg tcgcttggat ggcggaccaa
      241 acggacgacg atctgttcat caccgcgctg acgctcggcg aactgcgccg cggtatcttg
      301 gaaagaaact cgggacgcaa acgcagccag ctggagcgct ggttctcggg ccccgaaggc
      361 ccgcaatcgc tgttcgccaa ccgcattcta ccgtttgatg agaaagcagc cttgatatgg
      421 tcgcggctga tggccgaagg agcagcggcc ggaagcgcgc gcggcgcact cgatatgatc
      481 gtggccgccg tcgccgaggc gaatcattgc atcgtcgtca ccgacaatcc gaaacacttt
      541 gccggactca agacggtcac ccctacgaga taagcgaact agccgaggta gatatgttcc
      601 ggcgccaagt tggcaaggta tcgctcgtat gcccgcgtgt aggccatctc caaatgccgg
      661 gttagcgccg gcgtgtcgaa caagggtgtc ttgagcttat ttcgcgccaa tttttgcttg
      721 acctccgcca gccgcagcgg ttcggcagcg aactgcaccg ccaagtcctc gtactgcacc
      781 gcgcttgctg caatgagttc cggtaagccg attgtcttga gcagactcgc cgcaacgcgg
      841 ccggcgtagc attctccggg ccgcgtgagt accggtaatc ccgcccagag tgcgtcgatc
      901 gcggtcgcgt gagcattgta gggaagcgtg tcgagaaaca ggtccgcggc ccgatagcgg
      961 gccaggtgtt ccggcagcga agccatgcgc ggcgcgaaga ccaatcgatc cgcgtcgaca
     1021 cctcgctgca tggctgcctg ccgcaggtta ttcgcggccg cggcattacc ctgcgatagc
     1081 cacagcaccc cgtttgcggt ccggcgcagt atccgcatcc agctatcgaa tgtactcggc
     1141 atgatcttcg agttgttgtt gaaacagcaa aatacgattg catcaggcgg cagacccaag
     1201 tcgtggcgcg cgaataccgt agcggcgatc tcgcgggtag aatcgttggg caggaaacta
     1261 tgcggcaggt acacgatttt ctcggcgtag tgcccctgat gcgtttgcgg taccacggtt
     1321 cggtcgccga tcaaataatc catgtagacg gcgcccatcg tgccgggata gcccagatag
     1381 ctgatctgaa cgggtgcggc ccgcagagcg aatatcttcg tgcggctatg ctcggtaaat
     1441 ccgccgagat ccaccgcgat gtcgattccc ttgtcgcggg cgagccgcgc cacgtcggcg
     1501 tcggaccgat cccgcacgtc gataaattga tcgaaggctc gcaccagacg cgcgcgcatc
     1561 gcgtcgttcg ttgcgggacc aaaggaaaag gccgtgaagt cgaatttcga tcgatcatga
     1621 gtttcgaaca attgcgcgct cagcagcgac actggatgat gacgaaaatc ggcggagaaa
     1681 tatccgattc gcagcttatc gccgcgccgc ctcggcggga acgcaccgag cgaatcgtca
     1741 ggcggacatt cttctcgaac atagatctgt gccgccaagc gctgcaggat tggcgactcc
     1801 agcagcgcca acagcggaaa tggcgcggat acagccttgt gcgcctgcag cccggctgcc
     1861 agttgctgca aatccgctgt tagcccggac caatcgcaaa tctgcatttg cgattgccgg
     1921 cgcgtgccga gcagatactt gtgttctgga tcgagtgcaa gcgcttgggt gtagctcgac
     1981 gcagccgcct cgtaccgttc caactccttg agaatattcc cgcgattgaa ataggccgcg
     2041 aagtagtcgc ctttgagcgc gatggcttgg ctgtagctgc tgagtgcctc ctccaatcgc
     2101 ttgagatgtt tgagcacgga agcccggttg tagtacgcca gaaaatcgcc gggattgagt
     2161 gcgaccgctc gatcgtagct gccgagcgcc tcatcccatc ggcccatgcg ttccagcacc
     2221 gtgcctcgat tgcaaaaggc gtaggcatac gccggattta acgcaatggc tcgatcgtaa
     2281 tcggccagcg ccaattccca tcgtccaagt ccattcagag cattggcacg tttgtagtaa
     2341 gcctcggcgt gatcgggctt gcgatgaatt gcgctgtcgt atagttgaat ggccctctgc
     2401 aactggccgc tgcccagcgc ggcagccgcg ctcgcgtaca aagactgcca ctcatcggga
     2461 tcgatggact gcgataccat caaacgattt tatcattcga gcgcctgcaa agtgcgcaag
     2521 gtcggactga ccgccgctgc gcgactaggt atactcgagg aacactatga ccaagcctga
     2581 cacttcagcg gtcaggatcg aatccgccga gcacaccccc atgatgcagc agtacctgcg
     2641 catcaaggcg c
//
LOCUS       DASWZT010000547         4898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_38402, whole genome shotgun sequence.
ACCESSION   DASWZT010000547 DASWZT010000000
VERSION     DASWZT010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38402"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(329..1525)
                     /locus_tag="VGE92_15550"
     CDS             complement(329..1525)
                     /locus_tag="VGE92_15550"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY1315296.1"
                     /translation="MSLSKRVQKVKPSPTLAVTARAAQLKAEGKDIIGLGAGEPDFDT
                     PVHIADAGVEAIRKGITRYTSVDGTAELKDAIIAKFKRDNGLHYERSQILVSSGAKQT
                     CFNVCAALLDPGDECVIPAPYWVSYPDMALLADGVPVSVYAGPEQGYKITPAQLAAAI
                     TPRSKLIFINSPSNPTGAAYTKAELQALGAVLDKHPQIIIASDEMYEHIYWAAEPFTS
                     FAQANPQLYDRTVTINGVSKAYAMTGWRIGYCGGPKALVAAMATIQGQSTSNASSISQ
                     RASTVALNSDQSAVARMNKAFKERHDFVVAALNAIPGISCRPGAGTFYAFADVGSAMR
                     ALGMKDDNAFAEYLLVEAGVAVVPGSGFGAPGHMRISFACSMETLADALGRIKRVLAV
                     AEAKRA"
     gene            1681..3681
                     /gene="uvrB"
                     /locus_tag="VGE92_15555"
     CDS             1681..3681
                     /gene="uvrB"
                     /locus_tag="VGE92_15555"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEY1315297.1"
                     /translation="MSFELYSDYLPAGDQPQAIEKLVDGLSSGLAHQTLLGVTGSGKT
                     FTIANVIQQVQRPTLVLAHNKTLAAQLYGEFKVFFPKNSVEYFVSYYDYYQPEAYVPA
                     SDTFIEKDASINEHIEQMRLSATKALLERPDSIIVATVSAIYGLGDPEAYLSMVLHMS
                     RGDKLEQRKLLRRLADMQYTRNEIDLQQGTYRVRGDVIDIFPAESEREAVRVELFDDQ
                     IDNLSYFDPLTGEVLRKVPRLTIYPSTHYVTPRERIHQAVDEIRGELRTRLQELHAAG
                     KLVEAQRLQQRTLFDLEMMHEVGYCAGIENYSRYLSGRMAGEPPPCLFDYLPKDALLV
                     IDESHQTIPQLGAMYKGDRSRKETLVEYGFRLPSALDNRPLRFEEFEHLAPQSIYVSA
                     TPSHYEKRVSAQVIEQVVRPTGLTDPAVEIRPVRAQVDDLLSEIRLRAQANERVLVTT
                     LTKRMAEDLTEYLSEHGVKVRYLHSDIETVERSEIIRDLRLGKFDVIVGINLLREGLD
                     MPEVSLVAILDADKEGFLRSEGSLIQTIGRAARNLQGKAILYADVRTGSIERAVAETD
                     RRRNKQIEFNVEHGITPRTIVKAVADVMEGARSVQERERYGSQDDIAALAPEQAVKRI
                     KKLEAEMMKLARNLEFEQAARVRDQIQQLRQAAFGVPNSRVG"
     gene            3694..>4898
                     /locus_tag="VGE92_15560"
     CDS             3694..>4898
                     /locus_tag="VGE92_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315298.1"
                     /translation="MAKELTSSPDMDLDLDRTDRLPIIDASMLGPEQEDETLGLSSSS
                     LDHTAELAGGLIAAPSAPPEFMRVSAVDLPSLAESVRSVEERIARQNAEYEALTRAYE
                     RAREAESASVARANALSADLAAMKTALELEVGRTKDLDRAVTEKANAVEAARTRVEEA
                     LREANRFQSESRTLRDSLAARDATIKETAHSLGERDAQLVALQAEHSKIVPILEATSK
                     TSTQLDADLRATRGQANSLATELDSTRGTVAALTAQLKRGETLIKATRAELGAEKTKA
                     VSFLEMLRSRDWRRGFDLNMFREMDAKVGAAHVGLNALEADRDRLKAQVADLESQLAE
                     KTAAMQAETTRLSDALKGETSRLSAELAAEKARLTAEMEAQKARLTADIEAQKERLTA
                     ELAANAQALT"
BASE COUNT          998 a         1416 c         1556 g          928 t
ORIGIN      
        1 ctgtcgatat cgatggcgcg gccggtgctg tcgttgtaga tcaacaccgc ttgtttggcg
       61 ccgcgatcaa tcgcttcctt cgccgcgagc gcaaccgggg cgagcgcgcc cgtgatcaag
      121 cggcgcgagc caatgaatgc cgtgaattca acagcttggg gatcattcat tgcgagcgac
      181 tagtgattct ccaggcgcct aataataccc ggataaaatt agtaggtcaa tattatccgg
      241 gtattaatgc ccgtcagcct acgcgtcccg cgggttccct cggagctgcg cttcgcaaga
      301 tggctccgta ttcggcgttt atttggcctt aagcgcgctt cgcttctgcg accgcaagga
      361 ctctcttgat tcgaccgagt gcgtcggcca acgtctccat gctgcaggca aaggagatgc
      421 gcatgtggcc tggtgcgccg aacccggaac ccgggaccac cgcaacgccg gcttccacca
      481 acaagtactc ggcgaacgca ttgtcgtcct tcataccgag cgcgcgcatg gcactcccga
      541 cgtcggcgaa cgcatagaaa gtacccgcgc ccggccggca ggaaataccg ggaatggcat
      601 tgagtgctgc caccacgaag tcgtgtcgtt ccttgaaggc cttgttcatt cgcgcaacgg
      661 cgctctgatc gctgttgaga gccacggtgg aagcgcgctg cgaaatgctc gaggcgttcg
      721 atgtagactg accttggatg gtggccatcg cggccacgag ggccttcggt ccgccgcagt
      781 agccgatgcg ccagccggtc atcgcatagg ctttggagac gccgttgatg gtaacggtgc
      841 gatcatagag ttgcggattc gcctgggcga agctcgtgaa cggttcggcg gcccagtaga
      901 tgtgttcgta catttcatcc gaggcgatga tgatctgcgg gtgtttgtcg aggaccgcgc
      961 cgagcgcctg caactccgcc ttggtatagg cggcgccggt gggattgctc ggactgttga
     1021 tgaaaatcag tttactgcgc ggcgtaatcg ccgcggcgag ctgcgccggc gtgatcttgt
     1081 agccttgctc gggaccggcg tagacgctga caggaacgcc atccgccagc agtgccatat
     1141 cggggtagga cacccagtag ggcgccggga tgacgcactc atcacccgga tcgaggagcg
     1201 cggcgcatac attgaagcag gtctgtttgg cgcctgagga gaccaggatc tgactgcgtt
     1261 cgtagtgcag gccattgtcg cgcttgaatt tggcgatgat ggcgtccttt aattccgcgg
     1321 tgccgtccac ggatgtatag cgcgtgatgc ccttgcgaat tgcctcgacg cccgcatctg
     1381 ctatatgtac cggtgtgtcg aagtccggct cgccggcacc caaaccaatg atgtccttgc
     1441 cttccgcctt gagttgggcc gcccgggcgg tgacagccag cgtgggggag ggcttaacct
     1501 tttgaacgcg cttggaaagt gacactgcca agggagtttt cctatgttta aaacggcgag
     1561 cgagtgtacg aaaaatagcc gggggctgcc acttgacata attcaaatcg aaccgcggac
     1621 aagtgcacgg gtcgcgcacc gtctcgtgcc ggggttaaat caacgtgcga gaatcgactc
     1681 atgagttttg agttgtactc agattaccta ccggcgggcg atcaaccgca agcgattgaa
     1741 aagcttgtgg acggtctgtc gagcggactg gctcatcaga cgctgctcgg agtgaccggc
     1801 tccggcaaga cttttacgat cgccaacgta atccaacagg tgcagcggcc gacgctggtt
     1861 ctcgcgcaca acaagacgct cgcggcgcag ttatacggcg aattcaaagt atttttcccg
     1921 aaaaattcgg ttgagtactt tgtctcgtat tacgactact accagccgga ggcctacgtg
     1981 ccggcctcgg ataccttcat cgagaaggac gcgtcgatca acgaacacat cgaacagatg
     2041 cgtctctcgg cgaccaaggc gctgctcgag cggcctgatt cgatcatcgt cgcgacggtg
     2101 tcggccatct acggcttggg tgatcccgag gcttatttga gcatggtatt gcacatgtcg
     2161 cgcggcgata agctcgagca gcgcaagctc ctgcgacgtc tcgccgacat gcagtacacg
     2221 cgcaatgaaa tcgatttgca gcaaggcact tatcgcgttc gcggcgacgt catcgacatt
     2281 ttccccgcgg aatcggaacg cgaggcggtg cgcgtcgagc tgttcgacga ccagatcgac
     2341 aacctgagtt attttgatcc gctcaccggc gaggtgctgc gcaaggtgcc gcggctgacg
     2401 atttatccgt cgacgcacta cgtcacgccg cgcgagcgaa tacaccaggc ggtggatgag
     2461 atccgcggcg agctgcgcac gcggctccag gaattgcacg ctgccggcaa gctggtggag
     2521 gctcagcggc tgcagcagcg cacgctgttc gacctcgaaa tgatgcacga ggttggatat
     2581 tgcgcgggca tcgagaacta ctcgcggtat ttgtcggggc gcatggcggg cgaaccgccg
     2641 ccatgtttgt tcgattacct gccaaaagat gcattgctcg tgatcgacga gagccaccaa
     2701 accattccgc agctgggcgc gatgtacaag ggcgatcgct cgcgcaagga aaccttggtc
     2761 gagtacggct tccgtctgcc ctcggcgctc gacaaccggc cgctgcgctt cgaggagttc
     2821 gagcatctgg cgccgcaatc gatctatgtg tcggcgacgc cgtcacacta cgagaaacgc
     2881 gtgtcggcac aggtcatcga gcaggtggtg cggccgaccg gactcaccga tccggcggtc
     2941 gagatccgcc cggtgcgtgc gcaggtcgat gacctgctgt cggagataag attgcgcgcg
     3001 caggccaatg aacgcgtgct ggtcacgacg ctcaccaaac gtatggcgga agatttgacc
     3061 gagtacttga gcgaacatgg agtcaaggtt cgctacctgc attcggacat cgaaaccgtc
     3121 gagcgctcgg aaatcatccg cgatctgcgc ttgggtaaat tcgatgtgat cgtcggcatc
     3181 aatttattgc gcgaaggcct ggacatgccc gaagtgtcct tggtggccat cttggatgcc
     3241 gacaaggaag ggtttttgcg atcggaaggc tcgttgatcc agaccatcgg ccgcgccgcg
     3301 cgcaatctgc aaggcaaagc gatcctgtac gcggatgtca gaacgggctc gatcgagcgt
     3361 gcggtggccg agaccgaccg gcgccgcaac aagcagatcg aattcaatgt cgagcacggc
     3421 attacgccgc gcaccatcgt caaagcggtg gccgatgtga tggagggcgc gcgctcggtt
     3481 caagagcgtg agcgttacgg ttcgcaggac gatattgcgg cgctcgcccc cgaacaggcg
     3541 gtcaagcgca tcaagaaact ggaagctgag atgatgaaat tggctcgtaa cctcgagttc
     3601 gagcaggccg cgcgcgtgcg cgatcagatt caacaactgc gtcaagcggc gtttggcgtt
     3661 cccaattcgc gagtgggcta gcaagaggca aatatggcaa aggaacttac ttcttctccg
     3721 gacatggatc tcgacctcga caggacggat cgattgccaa taatcgatgc cagcatgctc
     3781 ggtcccgagc aggaagacga aaccctgggc ttgtcgtcat cctcgctcga ccatacggcg
     3841 gaactcgccg gaggactcat cgccgcgccg agcgcgccgc cggaattcat gcgcgtctcc
     3901 gctgtcgacc taccttcgct cgccgaaagc gtgcgctcgg tggaggagcg catcgcccgg
     3961 cagaatgcgg aatacgaagc cctgacgcgc gcctatgagc gcgcgcgcga agctgaatcg
     4021 gcctcggtgg cgcgtgccaa tgcactctcc gcggatttgg ccgcgatgaa gactgcgctc
     4081 gagttggagg ttggccgcac caaagatttg gaccgggcgg tgaccgagaa ggccaatgcc
     4141 gtggaggcag cgcgcacccg cgtcgaggag gcgttgcgcg aggcgaatcg cttccaaagc
     4201 gagtcacgca cattgcgcga ttcgttggcg gcacgcgatg ccacgatcaa agagacggcg
     4261 cattcgctcg gcgagcgcga tgcgcaactc gtggcgttgc aagccgagca ttcgaagatt
     4321 gtgccgatcc tcgaggccac ctcgaagacc tcgacgcaat tggatgcgga cctgcgcgca
     4381 acgcgcgggc aggcgaactc gcttgccacc gaactcgact cgacccgcgg cacggtggct
     4441 gcgctgacgg cgcagttgaa gcgcggcgag accctgatca aggcgacgcg agccgaattg
     4501 ggcgcggaga agaccaaggc ggtgtcattc ctcgaaatgc tgcgctcgcg cgattggcgc
     4561 cgcggcttcg atctgaatat gttccgcgag atggacgcca aggtcggtgc ggcgcacgtg
     4621 ggattgaacg cgctcgaggc ggaccgcgat cgcttgaaag cccaagtggc cgatttggag
     4681 tcgcagctgg ccgagaaaac ggccgccatg caggccgaga ccacccgctt gagcgatgca
     4741 ttgaagggcg agaccagccg cttgagcgct gaattggcgg ccgagaaagc ccgcttgacc
     4801 gctgaaatgg aagctcagaa agcccgcttg accgccgaca tcgaggcgca gaaggagcgc
     4861 ttgacggcgg aattggctgc caacgcgcag gccctgac
//
LOCUS       DASWZT010000548         6366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_91471, whole genome shotgun sequence.
ACCESSION   DASWZT010000548 DASWZT010000000
VERSION     DASWZT010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6366
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91471"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1384)
                     /locus_tag="VGE92_15565"
     CDS             complement(71..1384)
                     /locus_tag="VGE92_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315299.1"
                     /translation="MRCVFLLLLLLLAQAQVLAAEPPSRYGVDAPELAHLGRYAVGIK
                     TLRLVQRRQADVLAYDAARGSAPSMDRMLIVELWYPARPAARATGITYRASLPSEPPA
                     PPAQFSIPGIAVRDAPSIGTDHPLVIVSHGYSNDPYAMTWLTENLASKGYVVAAIRHE
                     DPPITDRTKFAEPLMRRPLDIAFVADALKGMLATEHLIDPGRTALVGYSMGGYGVLTA
                     AGATLDPQGGAVRLVPGGLLLPFARGGQSHDATLVKGLRAVVAISPAGGGTLAAWGAE
                     GLRSLSAPLLLIAGDSDRTVDYASGARAIFDAASGAQRYLLTFKGGGHAIGLSPVPDA
                     MRQRLWDQDWFEDPVWRKERISAINAHFITAFLDCYVMGDASRAAYLNVPVAESSGGV
                     WPATTPPLAYDAYSPGTVDISVWKGFQRNHAAGLELLQAQAGATR"
     gene            complement(1394..2113)
                     /locus_tag="VGE92_15570"
     CDS             complement(1394..2113)
                     /locus_tag="VGE92_15570"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: protein motif:HMM:NF009695.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEY1315300.1"
                     /translation="MSSPTGAHWRGHPLEAVLFDLDGTLLDTVADIALALNRTMLEYG
                     ARELPEDEVRRMIGRGSPILIERAVAWAGRAVDKTAQAAMVERFFHHYGELEESNEDR
                     AQPYPGAAEALRAVHDAGLKTAVVTNKQHRFAAALFKRLGLASQVDVLVGGDSCARRK
                     PDPQPLLFACQSLSVAPGAVLMVGDSLNDVQAARAAGIAVVCVSYGYNEGRDPRTLDC
                     DRLLDSLAELPPLLQIKPPTA"
     gene            complement(2106..2903)
                     /locus_tag="VGE92_15575"
     CDS             complement(2106..2903)
                     /locus_tag="VGE92_15575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY1315301.1"
                     /translation="MKIFSWNVNGIRAVLKKNLMQPFLDRYRPDILCLQETKAERGQF
                     EIDLPEYREYWNSALKKGYSGTAIFSKQEPLAVINGFPDQFAKRFAFADELRRDSAGE
                     GRVITAEFAKFYVVTVYTPNAKDDLSRLALRFKHWDPAFLAYCQQLEKKKPVVFCGDL
                     NVAHTELDLANPKPNRGKKGFTDEERAGFQNYLDAGFIDTFRLFTQGNGHYSWWSHFA
                     NSRARNVGWRIDYFLVSGKLRGAVKSAEIHADVMGSDHCPVSIVLDV"
     gene            complement(2928..4331)
                     /locus_tag="VGE92_15580"
     CDS             complement(2928..4331)
                     /locus_tag="VGE92_15580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY1315302.1"
                     /translation="MRNGLVCGVAAAALVAGCAVGPNYHRPDTPVDTHFANAAEPGLA
                     PGDPVEHYWSGFADPKLDSLIDDALAHNKGLAVAVANLQAARAARRLAGFDQFPTVTV
                     AGGYTHNLEAQQQLPGVPQPLREFDTAQGGFDGLWELDLFGRVRRNVEAARSDLGASA
                     ASLQDARVSVIAEVARDYFILRGLQDQLALTIRNADNQLNTTKLTRNRLEAGRGNELD
                     TSRAEAQWQTTLASLPTLQASIATTTYRLSVLTGRQPTALDEQLAPAPMPALPPLTSI
                     GTPEQLLRRRPDVRVAERRLAAATARVGVAMGDLFPKVTLVGEAGYWAPTFGDFGQSE
                     ARFFSVGPSISWAAFDLGRVRARISSAQAETDAALAGYEGAVLNALEDTEGALITYGR
                     SQSRRQALRVAAAASDKAADLARKRFEGGLIDFLEVLDAERTALSAELLLSQSRTDAA
                     TSLVAVYKALGGGWATR"
     gene            complement(4324..>6366)
                     /locus_tag="VGE92_15585"
     CDS             complement(4324..>6366)
                     /locus_tag="VGE92_15585"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1315303.1"
                     /translation="VAIIGTLAALLLAGFSINSLTLFGLVLATGIVVDDAIVVVENIE
                     RKIEQGLNPHAAAHAAMDEVTRPIISIALVLCAVFVPVAFVSGLTGQFYRQFGITIAA
                     STIISTFNSLTLSPALAALLLKPTNAPPDAFQRGINRVLGRFFALFNRGFERASRRYG
                     GGVRRIAAIRVAVLGVFVCLLISTWGIFRVVPGGFIPAQDKQYLIGIVQLPDAASLDR
                     TERVVRQVSQLATDTPGVAHAIGFSGLSVQGFVNLSNAAVLFFPLKPFDERTTKDLSA
                     GAIAAALNRKFGAIQDAVIFAVPPPPVQGLGTTGGFKLYLQDRGAHGYDELARVTAEV
                     LNKARQQRELNPYATYSTFQNSVPQLFADVDRVKAKNQGVPLSNVFDTLQAYLGSVYI
                     NDFNRFGRTYRVYAQAEPQFRNQPEDIANLKMRNLSGNMVPMGAVADVRFEAGPDRVS
                     HYNVYLASDINGQGSAGTSSSQASAAMERVLHDTLPTGFGYEWTDLTFQQKSAGNTAL
                     LVFPICVLLVFLILSAQYESWALPLAVILIVPMCLLSAMLGVWFKGSDNNVLTQIGFI
                     VLVGLACKNAILIVEFARERVEHGLAPLDAAIEACRLRLRPILMTSFAFIMGVIPLVT
                     ASGAGSEMRQAIGIAVFSGMLGVTLFGLFLTPVFFVTIEVWLERRRNRGAVEPEHA"
BASE COUNT         1192 a         2047 c         2029 g         1098 t
ORIGIN      
        1 gagaggcgcg ccaggcgagc tgacgaaagg ccgacgtcct gcggcgtcac gagcggcagc
       61 ggcggctctg tcatcgtgtc gcaccggctt gcgcttgcag caattccaag cccgccgcgt
      121 gattgcgctg gaagcctttc cagacgctga tgtcgactgt gccgggactg tacgcatcgt
      181 acgcgagggg cggcgtcgtc gccggccata ccccgcccga ggactcggcc accggaacgt
      241 tcagataggc ggcgcggctt gcatcgccca tgacgtagca gtcgagaaat gccgtgatga
      301 agtgcgcgtt gatggcgctg atgcgttcct tgcgccatac cgggtcttcg aaccaatcct
      361 ggtcccatag ccgctgccgc atggcgtcgg gcacggggct caagccgatg gcgtgtccgc
      421 cgcccttgaa ggtcaggagg tagcgttgcg cgccgctggc cgcatcgaat atcgcccgtg
      481 ccccgctcgc gtaatccacc gttcgatcac tgtcgccggc gatgagcagc agcggcgcgc
      541 tgagcgagcg cagtccctct gcgccccacg ccgcgagcgt gcccccgcct gcgggcgaga
      601 tcgcgacgac tgcgcgcaag cccttgacca acgtggcatc gtgcgattga ccgccgcgcg
      661 cgaacggcag cagcaatccg ccgggcacga gtctcaccgc cccgccttgc gggtcaaggg
      721 tcgcgccggc cgcggtcagc acgccataac cgcccatcga gtagccgacc agcgccgtgc
      781 ggccggggtc tatcagatgc tccgtggcca gcatgccctt gagggcgtcg gcaacgaagg
      841 cgatgtcgag aggccggcgc atcagcggct ccgcaaattt cgtccgatcg gtgatcggcg
      901 gatcctcgtg acggatcgcc gccacgacgt accccttgga ggcgaggttc tcggtcagcc
      961 aggtcatcgc atacgggtca ttgctatagc cgtgcgagac gatcaccagc ggatggtccg
     1021 ttccaatgga cggcgcatcg cgcacggcga tgccgggaat cgaaaattga gccggcggtg
     1081 cgggtggctc ggagggcagg ctagccctat aggtgatccc ggttgcgcgc gctgccgggc
     1141 gcgctgggta ccacaattcc acgatcagca tccgatccat ggacggggcg gaaccccttg
     1201 cggcatcgta agccagtacg tcggcttgtc tgcgctgcac caggcgcagc gttttgatcc
     1261 cgaccgcata acgcccgagg tgcgcgagtt ccggcgcgtc gacgccataa cggctcggcg
     1321 gctcggccgc gaggacttgc gcttgagcga gcagcagcag cagcagcaaa aatacgcatc
     1381 gcatgccgat agcttaagcg gtcgggggct tgatttgcag caacggcggt agctcggcca
     1441 acgaatcgag caagcggtca cagtcgagcg tgcgcggatc gcggccctcg ttgtatccgt
     1501 aggagacgca caccaccgcg atgccggctg cgcgcgccgc ttgcacatcg ttgagcgagt
     1561 cgccgaccat caataccgcg cccggagcga cactcaagga ttggcacgca aacagcaacg
     1621 gctgtggatc gggtttgcgc cgcgcgcagc tgtccccgcc gaccaaaaca tcgacttggc
     1681 tcgcgagtcc cagccgcttg aacagcgcgg cggcaaaacg atgctgcttg ttggtgacca
     1741 ccgcggtctt gagcccggcg tcgtgcacgg ccctgagcgc ctcggcagcc cccggatagg
     1801 gctgcgcgcg gtcttcattc gattcctcga gttcgccgta gtgatgaaaa aaccgctcga
     1861 ccatggccgc ctgcgcggtc ttgtccacgg ctcgaccggc ccacgccacg gcgcgctcga
     1921 tcaggatcgg cgagccgcgg ccgatcatgc gccgaacctc gtcctcgggc agctctcgag
     1981 cgccgtattc gagcatggtt ctgttgagcg ccaacgcgat gtcggccacg gtatcgagca
     2041 gggtgccgtc gagatcgaac agcaccgcct cgagggggtg gccgcgccaa tgtgcgcccg
     2101 tcggactaga catcgagcac gatgctcact ggacaatgat cgctgcccat cacgtccgcg
     2161 tggatctcgg cgctcttgac ggcgccgcgc aacttgcccg acaccagaaa ataatcgatt
     2221 ctccaaccga cgttgcgcgc ccgcgaattg gcgaaatggc tccaccacga ataatggccg
     2281 ttgccctgag tgaataggcg aaacgtgtcg atgaagcccg catccaggta attctgaaat
     2341 ccggcgcgct cttcgtccgt gaagcccttc ttgccgcggt tcggttttgg gttggcgaga
     2401 tcgagctcgg tatgcgctac attgagatca ccgcaaaaaa ccaccggttt tttcttctcg
     2461 agctgctggc aataggcgag aaaggccggg tcccaatgtt tgaagcgcag cgccaggcgg
     2521 ctcagatcat ccttggcatt cggcgtgtaa accgtcacga catagaattt cgcgaattcc
     2581 gcggtgatga cccggccttc cccggcgcta tcgcggcgta attcgtcggc gaaggcgaat
     2641 cgcttggcga attggtcggg aaacccgttg atgactgcga gcggctcctg cttcgagaat
     2701 attgcggttc cggaatagcc cttcttgagg gccgaattcc agtactcgcg atactcgggc
     2761 agatcgatct cgaactgtcc gcgctcggcc ttggtttctt gcagacacaa gatgtcaggc
     2821 cggtagcgat ccaagaacgg ctgcatcagg ttctttttca gaacggcgcg gatgccgttg
     2881 acattccaag agaaaatctt catgaccagt aacccattgg catgctctca gcgcgtcgcc
     2941 cagccgccgc cgagtgcctt gtagaccgcg accaaggacg tcgccgcatc ggtgcggctt
     3001 tgcgacagta acagctccgc gctcaaggct gtgcgttctg catcgagcac ttcgagaaaa
     3061 tcgatcaggc cgccttcgaa tcgcttgcgc gcaaggtcgg ccgctttatc gctcgccgcg
     3121 gccgcgactc gcagagcctg gcgtcgggac tgcgatcggc cgtaggtgat cagagcgcct
     3181 tccgtatcct cgagcgcgtt caaaacggct ccttcatacc cggcgagcgc cgcatctgtc
     3241 tcggcctggg ccgagctgat gcgcgcgcgc accctgccga ggtcgaacgc ggcccaggaa
     3301 atactcgggc cgaccgagaa aaaccgtgcc tcgctctggc cgaaatcccc gaaggtgggc
     3361 gcccaatatc cggcctcgcc gaccagggtg accttgggaa acaggtcgcc catcgcaacg
     3421 ccgacacgtg ccgtggcggc ggcgagccgg cgttcggcaa ctcgaacatc gggccggcgg
     3481 cgcagcagct gttccggcgt gccgatcgag gtcagcggcg gtaacgcagg catcggcgcc
     3541 ggcgccagtt gctcatcgag ggctgtcggt tggcggccgg tcaagacgct caagcgatag
     3601 gtggtcgtcg cgatcgaggc ctgcagagtc ggcagcgacg caagggtcgt ctgccattga
     3661 gcctcggcgc gcgaggtgtc caactcgttg ccgcgcccgg cctccaagcg gttgcgggtg
     3721 agcttggtgg tgttcagttg attgtcggcg ttgcggatcg tcaaggccag ttggtcctgc
     3781 aggccgcgca aaatgaagta gtcgcgggcc acttcggcaa tcacgctgac gcgtgcatcc
     3841 tgcaggctcg ccgcgctcgc gccgagatcg ctgcgcgccg cttcgacatt gcgcctgacg
     3901 cgcccgaata gatcgagctc ccatagaccg tcgaaaccgc cctgtgcggt gtcgaactcg
     3961 cgcagcggct gtggaacacc cggcaactgt tgttgcgcct ccaggttgtg cgtgtagccg
     4021 ccggccacgg tcacggtggg aaattgatcg aatccggcta gacgccgggc ggcacgtgct
     4081 gcctgcagat tggccaccgc caccgccaat cccttgttat gcgccaatgc atcgtcaatc
     4141 aaagagtcga gctttgggtc cgcaaacccg ctccagtagt gctcgaccgg gtcgcccggc
     4201 gcgagtccgg gctccgcggc attggcgaaa tgcgtatcca cgggcgtgtc cggccggtga
     4261 tagttcggcc ccacagcgca gccggcaacc agcgccgcgg cggccacgcc gcacaccaag
     4321 ccgttacgca tgttcaggct ccacggcgcc gcgatttctg cgtctctcga gccacacctc
     4381 gatggtgaca aagaagaccg gggtcaggaa cagaccgaac agcgtcaccc ccaacatgcc
     4441 cgaaaacacc gcgatgccga ttgcctggcg catctcgctg ccggctccgc ttgccgtgac
     4501 gagcggaatc acgcccatga taaatgcgaa ggaggtcatg aggatgggcc gcaggcgcaa
     4561 acggcacgct tcgatcgccg cgtcgagcgg cgcgaggccg tgttcgacgc gctcgcgcgc
     4621 gaactcgacg atcaggatgg cgttcttgca ggcaagtccc accagcacga tgaagccgat
     4681 ttgggtgagc acattgttgt ccgagccctt gaaccacacc cccaacatcg ccgacaacag
     4741 gcacatgggc acgatcagga tcaccgcgag cggcaaggcc cagctttcgt actgcgcgga
     4801 gagaatcaag aacaccaaca gcacgcagat cggaaacacg aggagcgcgg tattgcccgc
     4861 tgacttctgc tggaatgtca gatcggtcca ttcgtaaccg aagccggtgg gcagcgtatc
     4921 gtgcagcacc cgctccatgg ccgcgctggc ctggctggat gaggtaccag cagatccctg
     4981 cccgttgatg tcggatgcca ggtacacgtt gtagtgcgag acccgatccg gccctgcctc
     5041 gaagcgcacg tcggccaccg cgcccatcgg caccatgttg ccgctcaaat tgcgcatctt
     5101 gaggttggcg atgtcctccg gctggttgcg aaactgcggc tcggcttgag catacacccg
     5161 ataagttcgt ccgaaccgat tgaaatcatt gatgtaaacc gagccaagat acgcctgcag
     5221 cgtgtcgaag acattcgaca gcgggacgcc ctgatttttg gctttcaccc gatccacgtc
     5281 cgcaaacaac tgcggcacac tgttctggaa ggtcgagtac gtggcatacg gattgagctc
     5341 acgctgctgc cgagctttgt tcaacacctc ggcggtgacc cgcgccagtt cgtcgtagcc
     5401 atgggcgccg cggtcttgga gatagagctt gaatccaccg gtggtcccca ggccctgcac
     5461 cggcggcggc ggcaccgcaa agatcacggc gtcctgaatc gcaccgaact tgcggttgag
     5521 tgccgcggcg atggcgccgg cggacaggtc cttggtcgtg cgttcgtcga agggcttgag
     5581 cgggaagaac agcaccgctg cattactcaa gttgacgaag ccctgcactg acaaacctga
     5641 aaatccaatc gcatgagcga cgcccggcgt gtccgtcgcc agctgggaaa cctgccgcac
     5701 cactctttcg gtgcgatcga gcgaggccgc atcgggcagc tgcacgatgc cgatcagata
     5761 ttgtttatcc tgcgccggga tgaaaccgcc gggcacgacg cggaagatgc cccaagtcga
     5821 gatcagcagg cacacgaaca cgccgagcac cgccacgcgg atggccgcga tgcgccgcac
     5881 gccgccgccg tagcgccgcg atgcccgctc gaagccgcga ttgaagagtg cgaagaaccg
     5941 gcccagcacg cggttgatgc cgcgctggaa tgcgtccggc ggcgcgttcg tcggcttcag
     6001 cagcaatgcc gccagcgccg gcgacagggt cagtgaattg aaggtcgaga tgatggtgct
     6061 ggcggcgatg gtgatgccga attgacggta gaattgtccg gtgaggccgc tcacgaatgc
     6121 gaccggcacg aataccgcgc acagcaccag ggcgatggaa atgatcggcc gcgtcacctc
     6181 gtccatggcg gcgtgcgccg cggcgtgcgg gttcaggccc tgctcgattt tgcgttcgat
     6241 gttctccacc acgacgatgg cgtcatcgac cacgattccg gtggcgagaa ccaagccgaa
     6301 cagcgtcagc gaattgatcg agaaacccgc gagcagcaac gcagccaagg ttccgatgat
     6361 ggcgac
//
LOCUS       DASWZT010000549         6370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_38414, whole genome shotgun sequence.
ACCESSION   DASWZT010000549 DASWZT010000000
VERSION     DASWZT010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6370
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38414"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VGE92_15590"
     CDS             complement(<1..464)
                     /locus_tag="VGE92_15590"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY1315304.1"
                     /translation="MKTDTQLQLDVNAELAWDPAIKSGDISVKVTNGIVTLAGHVGSY
                     AEKMDAERATLRVAGVKALAVEIDVKLGGDSTRQDVDIARSATSVLEWTTYLPKDAVK
                     VTVEGGWVTLSGEVNWEYQRQSAVSAVRYLLGVKGVNDQVAIKPNISAPVVKA"
     gene            772..1293
                     /locus_tag="VGE92_15595"
     CDS             772..1293
                     /locus_tag="VGE92_15595"
                     /inference="COORDINATES: protein motif:HMM:NF024001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3761 domain-containing protein"
                     /protein_id="HEY1315305.1"
                     /translation="MLRKIGSISLIAGLVISAASMAQAPASAPAGSTGLCRDGTYWTG
                     ATKRGACHGHKGVQDWYATDAAPAAAAPAAAPAAAAAPAPAAAPAPAAAPAPAAGSAA
                     PAATKHSGSGWTPPATPAAGGGPGLVWVNTSSNVYHCQADKWYGKTKNGAYMSEADAK
                     AKGARPDHGKACQ"
     gene            complement(1361..2509)
                     /locus_tag="VGE92_15600"
     CDS             complement(1361..2509)
                     /locus_tag="VGE92_15600"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY1315306.1"
                     /translation="MTVRRVFLSPLWLLFSLPGYIGWRLLPPLAIGPFGVLAGITLLI
                     VFCVVIPLSVRQWTIRDPTLQNLLAWTGVLFMGFFSSLFVFTLLRDVALLFVRLLGSA
                     QQAAALAAPSARWTLALTVFVTLAGLVIARRRPHVVDIDIPVSNLPGALHGFSIVQIS
                     DVHVGPTIKRGFVEKIVSSVNGLRADLIAVTGDLVDGSVQQLSAHTAPLAGLAARHGA
                     FFVNGNHEYYSGERAWSAEIRRLGLTVLKNEHVVLEHDGALLVIAGVTDLSAHRFDAA
                     ERSDPAAALRGSPADAGARVLLAHQPNSAAAAADAGFDVQLSGHTHGGQFWPWNLFVG
                     FFQPFTAGLYRLRQMSIYVSRGTGYWGPPNRFGVPSEITRIRLVAATA"
     gene            2519..2716
                     /locus_tag="VGE92_15605"
     CDS             2519..2716
                     /locus_tag="VGE92_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315307.1"
                     /translation="MGAVRGQFKPATQRPAASRAGVRTPNIEPISIHGMARSIDRSFE
                     RLLPDAARISRRSGKHRDYHG"
     gene            2721..4046
                     /locus_tag="VGE92_15610"
     CDS             2721..4046
                     /locus_tag="VGE92_15610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY1315308.1"
                     /translation="MGPYVDSIVADETMPAVTGVVVIGGGIVGTCAALTLASRGVSVT
                     LCDKGYIACEQSSRNWGWCRQAGRDGREMPLIVQSLQLWRDMNRVTESDTGFRQCGVL
                     YVGESEHDESRFADWATMAKPYDIGTRIVRGAELAALMPGASRAYPCGLHVPTDGCAE
                     PQRAAPAIARAAQRKGATILLHCAVRGIERANGRAAAVITERGRIACDAVVLAGGAWS
                     SLFCASLGIRLPQLKVLSSVMRTAPIEGPDACTYLDAVGYRKRRDGGYTLARGMGYVA
                     PLVPDSLRYLRDFLPTIRKERGSLRVRVNAQSLRELRAPRNWPLDRPSPFEAARVLDP
                     EPNQTLNREALAAMIALYPQFRDAHIVQQWGGYIDVTPDVIPYISQVNSLPGLTVATG
                     FSGHGFGIGPAAGRLAAEMAIGATPCVDATAFRTSRFSDGSPIEIGPEI"
     gene            complement(4047..4862)
                     /locus_tag="VGE92_15615"
     CDS             complement(4047..4862)
                     /locus_tag="VGE92_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315309.1"
                     /translation="MQCSRFRASIAVTVLAWALPIAASAAAPDPALVSAVASPARSAS
                     AVARDAARHPVEELTFFGLTPTMTVVEVWPGGGYWTDILGPYLAQRGHYYIALPVPGD
                     AEEDGGVKRLRERLAAGKDRLGTVHETSLGPDHFDLAPPGSADLVVTFRNLHNWMDGG
                     YADAALRGAFKALKPGGILGIEEHRGRNDQPQDPKAKDGYVRQDFTIAMAKKAGFEFV
                     GSSEVNANPRDTKDWVDGVWTLPPTLSQGDKDRARYVAVGEADNYVLKFRKPK"
     gene            complement(4940..5614)
                     /locus_tag="VGE92_15620"
     CDS             complement(4940..5614)
                     /locus_tag="VGE92_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315310.1"
                     /translation="MNVGSKSLAALALAALAACGADEGNGSTKVNGSIHVAAGQQPGA
                     VGTVNGSIQIDEGATVTSATTVNGAVRLGDHASAGSLNNVNGAISLGSGAHVSGTVSS
                     VNGALTLQDGSEVAGSLSNVNGTISLVDAHAAAGIKTVNGSISISGASHVEGGILVDK
                     AGGSGLLNFGRTVPRIVIGPGATVQGDLRFERTVQLYVSDKATIGSVTGATPIPFSGD
                     TPPDQK"
     gene            5874..6248
                     /locus_tag="VGE92_15625"
     CDS             5874..6248
                     /locus_tag="VGE92_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315311.1"
                     /translation="MLTASQVSAVLGLSVSAGEHPIVSSLLLCSWAAPAKPQSVPKKV
                     SLSLMTERAFEVGKAALGDVAVTPLGAVGDDAYYTAARNLGTKLALKNGGVCAQIWVQ
                     GFAPAKAKALEKALALQMLAPH"
BASE COUNT         1216 a         2027 c         1991 g         1136 t
ORIGIN      
        1 gccttgacca cgggagcaga gatgttcggt ttgatggcga cctgatcgtt tacgcctttg
       61 acgcccaaga ggtagcgcac tgcactcacc gcggattgcc gctgatattc ccagttgacc
      121 tctccggata gagtaaccca gccgccttcc acggtgacct tgaccgcgtc cttcggcaga
      181 taggtcgtcc actccaggac gctggtagcc gagcgtgcaa tatcgacatc ttgtcgggta
      241 ctgtcgccac cgagcttcac atcgatttcc accgccagtg ctttgacacc ggcaacccgc
      301 aaagtcgccc gttcggcgtc catcttttcg gcgtaactgc ccacatgacc cgcaagagtc
      361 acgatgccgt ttgtaacttt cacggagatg tctccggact taatagcggg gtcccacgca
      421 agttcggcgt tgacatcgag ttgtaactga gtatcggttt tcatcgcgaa tatttcctgt
      481 gattgacgat gcacgaggct gcgcgtccca gccgcgtccg gtctgtgcgc gaccgcgctt
      541 agcagccaca tggttaagcg ccgctcgatc gacagcaaac cgatcctgtc gggaaacagg
      601 ccgtaacccc aagacaactg ggtcgggcgg caagacctgg tgggccaatc attacctaat
      661 cattaatttg acagtgtctt gcagttcagc gcgtctcgac ctggcatgct agtgaccctg
      721 actcgttggc atgacgacaa cgggcaaaac aaaataccta ggagatgcca aatgttgcgc
      781 aagattggtt cgatttcgct aatcgcaggt ctggtgattt cggctgcgag catggcacaa
      841 gcgccggcga gcgcgcccgc gggttccacc ggcctatgcc gcgatgggac gtattggacg
      901 ggagcgacca agcggggggc ttgtcacggt cacaagggtg tacaggattg gtacgcgacc
      961 gacgccgccc ctgcggccgc agcgcctgct gcagcaccgg ccgccgcggc tgcgccggca
     1021 cctgccgcgg cacccgctcc agcggctgcg ccagcgcccg ccgcaggatc tgcggctccg
     1081 gcggcgacca aacatagcgg cagtggctgg acgccgccgg caactcctgc cgccggaggc
     1141 ggaccgggcc tcgtctgggt gaacaccagc agcaacgtct atcactgcca ggctgataag
     1201 tggtacggga agaccaagaa cggcgcttat atgtccgaag cggacgcaaa agccaaaggc
     1261 gcccgtccgg atcacggcaa ggcttgccag tagccgtcgc atagggtcgg gagcgccgcc
     1321 gactcggcgc tcctgacctc tccacgcaga aattcaaacc ctacgctgtc gcggccacca
     1381 accgaatccg ggtaatctcc gacggcacgc cgaatcgatt cggcggtccc caatagcccg
     1441 tgccgcgact gacgtaaatc gacatttgtc tcagtcggta caggccggcc gtgaagggct
     1501 gaaagaaccc gacgaacagg ttccacggcc agaactgacc gccatgagta tggccggaca
     1561 gctgcacgtc gaagccggcg tccgccgccg ccgccgccga attgggctgg tgggcgagca
     1621 agactctggc gccggcgtcg gccggcgaac cgcgcagcgc cgccgcggga tcgctgcgct
     1681 cggcagcgtc aaatcgatgc gcgctgaggt cggtgacacc ggcgataacg agcaatgcgc
     1741 catcatgttc caatactacg tgctcgttct tgagcaccgt caagccgagg cggcggattt
     1801 cggccgacca cgcccgctcg cccgagtagt actcgtggtt gccgtttaca aagaaagcgc
     1861 catgacgcgc ggcaagcccg gcgaggggcg ccgtgtgggc ggacagttgc tgcaccgagc
     1921 cgtcgaccaa atcgccggtc acggcaatca aatccgccct caagccattg accgaagaga
     1981 cgatcttctc gacgaagccc cgcttgatcg tgggacctac atgcacatcg ctgatctgca
     2041 cgatcgaaaa gccgtgcaag gcgccgggaa gattgctgac cgggatgtcg atgtccacga
     2101 cgtgcggccg gcggcgggca atcaccaaac ccgccagggt cacgaaaacg gtgagtgcca
     2161 gagtccagcg cgccgacggc gctgcgaggg ccgcggcttg ctgcgcagat cccagcaggc
     2221 gcacgaacaa caatgcgacg tcgcgcagta aagtaaacac gaatagcgag gaaaagaatc
     2281 ccatgaacag aacgccggtc cacgccaaca gattttgcag cgtgggatcg cgaatcgtcc
     2341 attggcgtac cgagagcgga atgaccacgc agaaaacgat cagcaacgtg atcccggcca
     2401 gcacgccgaa aggtccgatg gccaagggcg gcagcagccg ccagccgatg tatccgggta
     2461 acgagaacaa cagccacagc gggctcaaga agacgcggcg cacagtcacc gagcgctaat
     2521 gggggcggtg cgcgggcaat tcaagcccgc gacgcagcgc ccggcggcgt cgcgtgcggg
     2581 cgtgcgcacc ccgaacatcg agccgatatc gatacatgga atggcgcgat ccatcgacag
     2641 atccttcgag cgcctgttgc ccgacgccgc tagaatatcc cggcgcagcg gcaaacaccg
     2701 cgactaccac ggatgaaatc atgggtcctt acgttgattc catcgtcgcc gatgaaacga
     2761 tgcccgccgt taccggcgtc gtcgtgatcg gcggcggaat tgtcggcacg tgcgccgcgc
     2821 tcactctcgc gagccgcggc gtttccgtca cgctgtgcga caagggctat atcgcctgcg
     2881 agcagtccag ccgaaattgg ggctggtgcc gccaggcggg ccgcgacggc cgggaaatgc
     2941 cgctgatcgt gcagagtctg cagttatggc gcgacatgaa ccgggtcacg gaaagcgaca
     3001 cgggatttcg ccagtgcggc gtcctgtatg tgggtgagag cgaacacgat gagtcgcgtt
     3061 ttgcggattg ggcgacgatg gcgaaaccct acgacatcgg aacccgcatc gtgcgcggcg
     3121 cggaactcgc ggcactcatg cccggcgcaa gccgcgccta cccctgcggc ttgcacgtgc
     3181 cgaccgatgg ctgcgccgag ccgcagcggg cagcgcccgc gatcgcgcgc gccgcccagc
     3241 gcaaaggcgc gactatcctg ctccactgtg cggtgcgcgg catcgagcgg gcaaacggcc
     3301 gcgccgccgc tgtgatcacc gagcggggac gtatcgcatg cgatgcagtc gttctggccg
     3361 gcggtgcgtg gtcgagtttg ttctgcgcga gtctcggcat ccgtctgccg caattgaagg
     3421 tgttgtcctc tgtgatgcgc acggcgccga tcgaggggcc cgatgcatgc acgtacttag
     3481 atgcggtcgg ctaccgcaag cggcgcgatg gcggctatac gctcgcccgc gggatgggct
     3541 atgtcgcgcc gctggtaccg gactcgttgc gctatttgcg cgatttcttg ccgaccatcc
     3601 gcaaggaacg cggctcgttg cgagtccgcg tgaatgcgca atcgctgcgc gagctgcgcg
     3661 cgccccgcaa ttggccgttg gatcggccga gtccgttcga agcggcgcgg gtgttggacc
     3721 ccgagcccaa tcaaaccctc aaccgcgaag cgctggcggc tatgattgcc ctgtatccgc
     3781 aatttcgtga tgcgcacatc gtgcagcaat ggggcggata catcgatgtc acgccggacg
     3841 tgattcccta tataagccaa gtgaattcgc tgcccggact gaccgtggcc accggatttt
     3901 cggggcatgg gtttggaatt ggtccggcgg ccggacgcct cgcggcggaa atggcgatcg
     3961 gcgcgacccc ttgcgtcgac gcgacagcgt ttcgcacttc tcgattcagc gatggctcgc
     4021 cgatcgagat cggaccggaa atctgatcac ttgggcttgc ggaacttgag cacgtaatta
     4081 tcggcctcgc cgaccgcaac atagcgcgca cgatctttgt cgccctgcga gagagtgggc
     4141 ggcagagtcc acacgccgtc gacccaatct ttggtgtcac gaggatttgc attcacctcc
     4201 gatgagccga caaactcgaa cccggccttt tttgccatgg caatcgtaaa atcttggcgg
     4261 acgtagccgt ctttggcctt cggatcctgc ggttggtcgt tgcggccgcg gtgctcttcg
     4321 atgccgagaa tgccgccggg tttcagcgcc ttgaacgccc ccctcagcgc tgcgtcggca
     4381 tagccgccat ccatccaatt atgcaaattg cgaaacgtca cgaccagatc ggcggagccc
     4441 ggcggagcca aatcgaagtg gtcgggcccc aggctcgttt catgtacggt gcccagccga
     4501 tccttccctg cggcgagtcg ttcgcgaagg cgtttcacgc cgccatcctc ctcggcgtcg
     4561 ccagggacgg gcaacgcaat gtagtaatga ccgcgctggg cgagatacgg gccaaggata
     4621 tcggtccaat agcctccgcc cggccacact tcgaccacgg tcatcgtggg tgtaagcccg
     4681 aagaatgtca gttcttcgac gggatggcgc gccgcgtcgc gcgcgacggc cgaggcactg
     4741 cgcgccggac tcgccaccgc gctgacgagt gcgggatcag gggcggcagc cgaggcagcg
     4801 atgggcagcg cccacgcgag gacggtaaca gcgattgatg cgcggaagcg ggagcattgc
     4861 atggctatac ccttctcgat gcgttgattg agccgcagta tcataaattc accgccaaaa
     4921 cgtgatgttt tcgccccagt cacttctgat ccggcggagt atcgccgctg aacgggatcg
     4981 gcgtcgcgcc tgtcacgctg ccgatggttg ctttgtcgct gacgtaaagc tgcaccgtgc
     5041 gctcgaatcg taagtcgcct tgcacggtgg ctcccggtcc gatcacgatg cgaggcaccg
     5101 ttcggccaaa attcagcaat ccgctgccgc cggccttgtc gaccagaatg ccgccctcaa
     5161 cgtgcgaggc acccgagatg ctgatgctgc cattgacagt cttgatgccc gcggccgcat
     5221 gcgcgtcaac cagcgaaatt gtaccgttca cgttcgacag cgagccggcg acttccgacc
     5281 cgtcctgcag cgtgagggcg ccgttgaccg aggaaacagt ccccgacaca tgcgcaccgc
     5341 tgccgagcga aatcgcacca ttgacgttgt tcagcgaccc agcgctcgcg tgatctccca
     5401 gacgcaccgc gccgttcact gttgtagcgc tggtgaccgt ggcgccctca tcgatctgaa
     5461 tcgagccgtt gaccgtgccg acggcgccgg gttgctggcc ggctgccaca tgaatggaac
     5521 cgttgacctt ggtcgagccg ttaccctcgt ccgcgccgca cgccgccagc gccgcaaggg
     5581 caagggccgc aagggatttg cttcctacgt tcatgacggc tccccgagac tggtactcgg
     5641 ctgctactgt atcagtatcg gttaatagct caaccagcag agcacgcgtg ctcggcgcgg
     5701 cgatctgcaa cgcctataat caattctggt cagtcgaggg cgatcgctac atgcccatca
     5761 tttccagcag gagcagctta gcagcgttgc ctttcgcttg tgcagtctgc ctgtgtccga
     5821 ttgtgccgca agcggtgcgc gcggcgccgc cccctggcgc ggatgcctgt gcaatgctca
     5881 ccgcgtcaca ggtgagcgcg gtgctgggtc tgtcggtatc cgcgggcgag cacccgattg
     5941 tgtccagctt gctgctttgc agttgggcgg cgcctgcgaa accgcaatct gtccccaaga
     6001 aagtgtcgct cagtttgatg accgaacgcg ccttcgaagt cggcaaagct gcgctcggcg
     6061 acgtcgctgt gacgccgctc ggcgcggtgg gcgacgatgc ttactacacc gccgcgcgca
     6121 atctcggcac caagcttgcg ctcaagaatg gcggcgtctg cgcacaaatc tgggtgcagg
     6181 gcttcgctcc ggcaaaggct aaagctctgg agaaggcatt ggcgctgcaa atgctggcgc
     6241 cgcactgagg tctcttgaca agtcgcgtcg ccttgcggac acttcaccat atggtgaact
     6301 ataacgcaca gctcgaccga actttcgcgg cactcgttga cccgacacgg cgcgccattc
     6361 tggcgcggct
//
LOCUS       DASWZT010000550         7284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_4650, whole genome shotgun sequence.
ACCESSION   DASWZT010000550 DASWZT010000000
VERSION     DASWZT010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7284
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4650"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VGE92_15630"
     CDS             <1..319
                     /locus_tag="VGE92_15630"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY1315312.1"
                     /translation="SVTVVKAAAPGPESPPTPAVMTTFTQVAQSMWPGLIVMPSMDAG
                     ASDSKYTRAAGMPSYGATSNFFDLEDMRAHGQDERIKSDRFAEGTEFAYRLMKAFSAA
                     GAP"
     gene            complement(331..1095)
                     /locus_tag="VGE92_15635"
     CDS             complement(331..1095)
                     /locus_tag="VGE92_15635"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY1315313.1"
                     /translation="MGGMTDNLPEARFFRLKFLPLAVVAALGLVVPYLAAYLAFLSSE
                     VLHTPSPHGATLPWLYAQHALQLLLALAAIAALKRRVPADYGLHWPREKTYILPAVLW
                     GAAFGVIMTLVDYAPQLSAHTKPDPGFPLTSTSVIGWLFFESVYVGPTEEIPFRSLLV
                     TYLAATMPGKLRLGRFSMNWAGVIVALIFALLHARNFNLHAWPIALGQQIYAFALGVL
                     YAYWLEKSNSVVAPIVGHNVSDGVEYAIVFIWILLQ"
     gene            1589..1864
                     /locus_tag="VGE92_15640"
     CDS             1589..1864
                     /locus_tag="VGE92_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315314.1"
                     /translation="MSSQAQSQSRQDFSCTLGSMKRIVSIVTFAPSDRQPRGACRVDY
                     TKEGVTKELFSSGTGHAYCTKQATVLVTKLGEAHYSCRLQTPDEPAK"
     gene            2109..2252
                     /locus_tag="VGE92_15645"
     CDS             2109..2252
                     /locus_tag="VGE92_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315315.1"
                     /translation="MPVLLTTGYSSDASPKSGWKDIFVLHKPYDIHSLDVALRTALGR
                     KPT"
     gene            2271..3074
                     /locus_tag="VGE92_15650"
     CDS             2271..3074
                     /locus_tag="VGE92_15650"
                     /inference="COORDINATES: protein motif:HMM:NF005699.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEY1315316.1"
                     /translation="MNSRVSVAIDDGVAAVRLSRPEKMNALDDLMFQALIESGERLRH
                     EPKLRAVVLSGDGKAFCGGIDLGSLEQLGSQAPDALTRRPYGLTNMFQHAAWVWHDLP
                     VPVIAAVHGVAFGGGFQIMLGADMRIAAADARFSVMEIRWGLVPDMAGTLLMRQLARD
                     DIVRELTYTGRIFSATEAQSYGFVTRVSDDPLADALALAKDIARQSPDAVREAKRLFN
                     GLSPVATLADGLRAESDAQGRLIGSFNQREAVSAGFQKRPPLFKDAGAD"
     gene            3151..3630
                     /locus_tag="VGE92_15655"
     CDS             3151..3630
                     /locus_tag="VGE92_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY1315317.1"
                     /translation="MPYQLDINGRIREVDVPENMPLLWVLRDELGLVGTKFGCGAGLC
                     GACTVHLNGAAQRSCQLPVSAVGASKITTIEQVGETPIGAKLQSAWLEIDVMQCGYCQ
                     AGQIMSASALLHQTPKPSIEQINAAMAGNICRCACYNRIQTAIQMAAGVRQELAHDA"
     gene            3620..5824
                     /locus_tag="VGE92_15660"
     CDS             3620..5824
                     /locus_tag="VGE92_15660"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY1315318.1"
                     /translation="MTREFGLDNLERRTVLKASLLAGGGLLLDAVFPMRADALSALGS
                     PADASVLSAFVSVAPDGIVTIMSKNPEIGQGIKTSLPMIIADELDCDWSQVRIQQADV
                     DEKRYGQQVAGGSRATPVNWLPMREIGAAARAMLIQAAAQKWSLDSKELTTEKGRIFH
                     AATRRSIGYGEVASSAARLSPPDPKTLTLKTPAQFTIIGRSRVGVDSAKIVKGQPIFG
                     IDTRLPGMLHAVYEVAPAHGGRLLKADLEAAQRAPGVKHVLAIPGNGDADGGLADGVA
                     ILATNWWLANEARSKLKIDWDLSAAQGHSSSVYQAKATAAMDAGGGVELRREGDAARV
                     LASAATRLSARYQYPFIAHVPMEPQNCAARFKDGKLEIWAPTQAPGAGLAAVQKVLGL
                     APENITIHLTRMGGGFGRRLLNDYMVQAAAIAKAVPGTPIQLLWSREDDVRRDYYRPA
                     GWHHFEAGIDAHGKLVAFTDHFVTFGDGKRATRGGDMNTSMVPAGIVENLLFTQSMMP
                     TAATTGWLRAPGSNALCFVSQSFLDEAAIAAGSDLPTFTLAMLGERREIAPLDGRGPP
                     FDTGRARDVIDKVVQTSRWTEKPQQPGRAKGFAFYFCHQGYFAEVVDASVADGRIQVH
                     TVWVAGDVGSQLINPMGAENQVRGAIIDGLAQAFAGQRIEFVDGVVQQSNFDNFAVAR
                     ISSTPQIEIAWLKPDKPPTGLGEPSLPPVIPALTNALFAATGKRLRSLPIQL"
     gene            complement(5831..6235)
                     /locus_tag="VGE92_15665"
     CDS             complement(5831..6235)
                     /locus_tag="VGE92_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315319.1"
                     /translation="MSYDLFVGCFVDGEKARFPRERLESYFGPYITRREQNCSTLDFG
                     AHGTSYLYCADQIQIDGFSINRPVGAPELFQALFDALRAGPLVLFAPGDCPPLIGDAQ
                     HSVHVPAAMVAALGTPVVLRSAAEILQRIATA"
     gene            complement(6272..7000)
                     /locus_tag="VGE92_15670"
     CDS             complement(6272..7000)
                     /locus_tag="VGE92_15670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY1315320.1"
                     /translation="MVTGGGRGIGREAALLLAAAGMRVMIVARTAAELAGVGLDYVVA
                     DLGTAAGCAEAVGETERRLGPIDVLVVNHGIGSAHERVVWEQDPEVWRETMRVNLDGP
                     FELARLTTGGMCKRGFGRLVFTSSTAGEKAERAGSAYTASKHGVIGLARAVAQDAGPF
                     GVTSNAVLPGWVRTSMAERSAKAEAERRGVSVQQVWDERAALYPQKRVLEPREVADVI
                     AFLCSDAAGGVNGEAITVALGGLW"
BASE COUNT         1418 a         2315 c         2253 g         1298 t
ORIGIN      
        1 gagtgtaacc gtcgttaaag cggcagctcc gggtcctgag tcgccgccga ccccggccgt
       61 catgacgacg ttcacccaag tcgcgcagtc gatgtggcct gggctgatcg tgatgccgag
      121 catggacgcc ggcgcatcgg attcgaagta cacgcgcgcc gcgggcatgc cgagttacgg
      181 cgccacgtcg aatttcttcg acctcgagga catgcgcgcg cacgggcagg atgagcgcat
      241 aaaatccgat cgcttcgcgg aaggaacaga attcgcttat cggctgatga aggcctttag
      301 cgctgcaggc gcaccctgag cggtcgaggt ttattgcaac aaaatccaga tgaaaacgat
      361 cgcgtattcg acgccgtcac tcacgttgtg gccgacgatc ggcgccacca cgctgttgga
      421 tttttccagc caataggcat agagcacgcc tagcgcgaag gcgtaaatct gttggccgag
      481 cgcgatcggc catgcatgca gattgaaatt cctggcgtgc agcagtgcga agatgagcgc
      541 gacgatcacg cccgcccagt tcatgctgaa gcgtcccagg cgtaacttgc ccggcatcgt
      601 ggcggccaga taagtgacca gcaacgacct gaacggaatt tcctccgtgg gtccgacgta
      661 gacactttcg aagaaaagcc agccaatgac actcgtggat gtgagcggaa atcccggatc
      721 aggcttggtg tgtgcgctga gctgcggcgc atagtcgacc agcgtcatga tcacgccgaa
      781 tgcggcgccc cacagcaccg caggcaagat gtaggttttc tcgcgcggcc agtgcaatcc
      841 ataatcggcc ggcacccgcc gtttgagcgc ggcgattgcg gccagagcga gcagcagttg
      901 cagggcgtgc tgcgcataca gccacggcag cgtcgcccca tgcggcgatg gtgtatgcag
      961 tacttcgctg ctcaagaacg cgaggtaagc cgcgagatac ggcactacca ggcccaacgc
     1021 cgcaacgacg gcgaggggca agaacttgag gcgaaaaaaa cgcgcctcgg gcagattgtc
     1081 cgtcataccg cccatggaaa gaccacccgt tgctttatcg cgtgaggttg gaccgcagcg
     1141 gcacaatcgt caaccaagac gttccgaatc tcggttgagc tccgccacga ctatgaccga
     1201 aacgcaaaga ggcgcgcctc cggcgccgtg cgcgtgcggc ttgccgcggt gatgagcttg
     1261 ttggccgaca tcgagaatgt ggcgcggccg tcgcgcaaat ccatctttcg cgtcaggccc
     1321 acgttaagtt atgtcgcgcg gatttcgtga ataggctcat gcgacttcct aaagatgccg
     1381 agcatggatg aacccgagag ctcgctacgc aggacatttg tgtcgttcga ggtaggcgcc
     1441 cggcgcagct cattgggttg gatttgtcgc attcgtcttc ggtgagtcgc ggcgcgatct
     1501 ataatcgaag gatcacccta ttggtcgagc tcatatgatg ttccggcact ctcctcaact
     1561 actcggcggt cttttgatcg gtctgttcat gagcagtcaa gcgcagtcgc agtcccgcca
     1621 ggacttttcc tgcaccctgg gctcgatgaa acgaatcgtc agcatcgtca ccttcgcccc
     1681 gtcggatcgg cagccgcgcg gcgcctgccg ggtcgattac acgaaggagg gtgtcaccaa
     1741 ggaattattt tcatcgggaa ccggccatgc ctattgcacc aagcaagcga cggtgctcgt
     1801 gacgaagctc ggcgaagcgc actattcctg ccgtctgcaa acgccggatg aacccgccaa
     1861 atagtctcga gcgaaccccc accatcgcaa agcccggcat tgaaaattct acaaagcccg
     1921 gcctgactca cctgatgcgc cagcccttga aaacttggcc cgcgaaatta caaagatata
     1981 ctgatcaggt tcaatgaccg acgataagaa cgccttatgt ccgcacgacc gatgagtccc
     2041 gcagaaatcc ccgacgcgca gatgacaccc gcatttctgc aaggtggcgg ctgcggcgcc
     2101 ggccgcactt gccggtgttg ctcacgacgg gatattcgag cgatgcgtca ccgaagagcg
     2161 gctggaagga catcttcgtg ctgcataaac cgtacgatat ccactcgctc gacgtcgcgc
     2221 tgcgcaccgc actcggtaga aagccgacgt gaccaccagg gaggtttcga atgaatagcc
     2281 gagtcagcgt tgcaatcgac gacggtgtcg ccgctgtgcg gctgtctcgc cccgagaaaa
     2341 tgaatgcgct cgacgatctc atgtttcaag ccctcatcga gtcgggcgag cgcttgcgac
     2401 acgaacccaa gttgcgcgcg gtcgtcctat ccggcgacgg taaggcgttc tgcgggggaa
     2461 tcgatctcgg gagcctggaa caactcggca gccaggcgcc cgacgcgctg acgcgccgcc
     2521 cctacgggct gactaatatg tttcagcacg ccgcgtgggt ctggcatgat cttcccgtgc
     2581 cggtgattgc tgcggtacat ggcgtcgcgt tcggcggcgg atttcagatc atgctcggcg
     2641 ccgacatgcg catcgccgcg gcggatgccc gcttctcggt gatggaaatc cgctgggggc
     2701 tggttccgga catggccgga accttgttga tgcgtcagct tgcgcgcgat gacatcgtcc
     2761 gcgaactgac ttataccggc aggatattct ccgccaccga ggcgcagagc tacggcttcg
     2821 tcacccgcgt gagcgacgac ccgcttgccg acgctctagc cctcgcgaag gacatcgccc
     2881 gccaaagccc ggacgccgtc cgcgaggcta aacggctctt caacgggctt agtcccgtgg
     2941 caacgctcgc ggacggattg cgtgcggagt cggacgcgca gggtcgtctc attggctctt
     3001 tcaatcagcg ggaagcggtc agcgcgggat ttcaaaagcg gccgccgctt ttcaaggatg
     3061 ccggcgccga ctaaacgctg cagcgccgcg ttggcaggta cggccgaagc gcatatagtc
     3121 gaattcacca cccgcatagg aatcttcggc gtgccgtatc aacttgacat caatggcagg
     3181 attcgcgaag tcgacgtgcc cgagaacatg ccgctcctct gggtgctgcg tgatgagctc
     3241 gggttggtcg gcaccaagtt tggctgtggc gccggtctgt gcggcgcctg cacggtacat
     3301 ctcaatggcg cagcgcagcg ttcgtgtcag ctccccgtga gcgccgtcgg cgcctcgaaa
     3361 atcaccacga ttgaacaagt cggtgagacg ccgatcggtg ccaagctgca gagtgcgtgg
     3421 ctggaaatcg acgtgatgca atgcggatat tgtcaagccg ggcagatcat gtcggcgagc
     3481 gccttgctgc accaaacgcc caagcccagt atcgagcaga tcaatgccgc catggcgggg
     3541 aatatctgcc gctgcgcctg ctacaaccgc atccagaccg ccatccaaat ggccgccggc
     3601 gttcgccagg agctcgctca tgacgcgtga gttcgggctc gacaatctcg aacggcgcac
     3661 cgtgctcaag gcctcgctgt tggcgggcgg cggcctgttg ctcgacgccg tattcccgat
     3721 gcgcgccgat gcgttgagtg cattgggctc acccgccgat gcgtcggtgc tatcggcatt
     3781 cgtgtcggtc gcgcccgacg ggatcgtgac catcatgtcg aagaatccgg agatcgggca
     3841 gggcatcaag acctcgcttc ccatgatcat cgcggacgag ctcgactgcg attggagcca
     3901 ggtgcggatc cagcaggctg acgtcgatga aaaacgctac gggcagcaag tggccggcgg
     3961 tagccgggca acgcccgtca attggctgcc gatgcgcgag atcggtgccg cggcgcgcgc
     4021 gatgctcatt caagccgccg cgcaaaaatg gtcgctcgat agcaaggaat tgacgaccga
     4081 gaagggacgg atctttcacg ccgccacccg gcgctcgatc ggttacggcg aggtcgcctc
     4141 ctcggccgcc cgattgtcgc cgccagaccc gaagacactc acgctcaaga caccggcgca
     4201 attcaccatc attggtcgtt cacgggtggg cgtcgacagc gccaagatcg tgaaggggca
     4261 acccattttc ggcatcgaca cacgcctgcc cggcatgctg catgcggtgt acgaagtggc
     4321 gccggcgcat ggcggtcgcc tgctcaaggc cgatctcgaa gcagcccaac gggcgcccgg
     4381 cgtgaaacac gtcctggcga ttccgggcaa tggagatgcc gatggcggcc tcgcggacgg
     4441 ggtggccatt cttgcgacca attggtggct cgccaacgaa gcgcgctcga agctcaagat
     4501 cgactgggat ttgagtgccg cccagggtca tagcagctcg gtctaccaag ccaaggcgac
     4561 cgccgcgatg gacgcggggg gtggcgtgga gttgcgccgt gaaggcgatg ccgcgcgagt
     4621 gctcgcatcg gccgccactc gcctgtcggc gcgctatcaa tatcccttca tcgcccacgt
     4681 accgatggag ccgcaaaatt gcgccgcacg cttcaaggat ggaaagctcg aaatttgggc
     4741 tcccacccag gcgccgggcg cagggttggc cgccgttcag aaagtactcg ggcttgcgcc
     4801 cgagaacatc accattcacc tcacgcgaat gggtggcgga ttcggccgcc gtttgttgaa
     4861 tgattacatg gtgcaggcag cagcgatcgc gaaggcagtg cccggcacgc cgattcaact
     4921 cttgtggtcg cgcgaggacg atgtgcggcg cgactactat cgacccgccg gctggcacca
     4981 ctttgaagcg ggaatcgacg cgcacggcaa gctggtcgcc ttcaccgatc acttcgtgac
     5041 gttcggcgac ggcaagcgcg caacgcgcgg cggcgacatg aatacctcga tggtgcctgc
     5101 gggaatcgtg gaaaacctgc tgttcacgca atccatgatg ccgacagccg ccaccactgg
     5161 gtggctgcgg gcgccgggat cgaatgcgct gtgtttcgtg tcgcagtcct tcttggacga
     5221 ggccgccatc gccgccggct ccgacttgcc gaccttcaca ctcgcgatgc tgggcgagcg
     5281 ccgcgagatc gcgccgctcg acggccgcgg gccgcccttc gacaccggac gcgcgcgcga
     5341 cgtcatcgac aaggtggtgc agacttcgcg ctggacggaa aagccgcagc aaccgggccg
     5401 ggccaagggc ttcgcgtttt atttttgcca tcagggctat tttgccgagg tggtcgatgc
     5461 cagcgtggcc gacggccgga tccaggttca cacggtgtgg gtggcgggcg atgtcggcag
     5521 tcaattgatc aatcccatgg gtgccgagaa ccaggtgcgg ggtgccatca tcgacggctt
     5581 ggcgcaggcg tttgcagggc aacgcatcga attcgtcgac ggcgtcgtcc agcagagcaa
     5641 ctttgataac tttgccgtgg cacgcatttc aagtacgccg cagatcgaga tcgcgtggct
     5701 caagcccgac aagccgccga ccggtttggg ggagccctcg ctgccacccg tgattcctgc
     5761 cctgaccaac gcactgtttg ccgcgacggg aaaacggctg cgctcgctgc cgattcaact
     5821 gtgaagcggc ctacgccgtt gcgatgcgct gcaagatttc agctgcggac ctgagcacca
     5881 ccggcgtccc cagcgccgcg accatcgcag ccggaacatg caccgagtgt tgggcgtcgc
     5941 cgatcaacgg tggacagtcg ccgggcgcaa acaggacgag aggccccgcg cgcagcgcgt
     6001 cgaacaaggc ttgaaacagc tccggggcac cgaccgggcg attgatactg aatccatcaa
     6061 tctggatctg atcggcgcag tacagataag acgtaccatg ggccccgaag tcgagcgtcg
     6121 agcagttctg ctcgcggcgc gtgatatacg ggccgaaata gctctcgagg cgctcgcgtg
     6181 gaaagcgcgc cttctcgccg tcaacgaagc acccgacgaa aagatcgtag ctcaagggga
     6241 gccgatgacc ggcgaataaa tcggcattcc cctaccagag gccgcccaac gccacggtga
     6301 ttgcctcccc attcacgcca cctgccgcat cgctgcatag aaacgcaatc acgtcggcaa
     6361 cctcgcgcgg ctccaatacc cgtttctgcg gatagagtgc agcgcgctcg tcccacacct
     6421 gttgaacgct cactccgcgt cgctcggcct cggctttggc cgagcgctcg gccatggacg
     6481 tgcgcaccca gccgggcagc acggcattcg aggtgacgcc gaacggcccc gcatcctgcg
     6541 cgacggcgcg tgccagtccg ataactccgt gcttggacgc ggtgtaggcg ctgcccgctc
     6601 gttccgcttt ctcgcccgcg gtcgaactcg tgaacacgag ccggccgaag ccgcgtttgc
     6661 acataccgcc ggtggtgagt cgcgcgagct cgaatggccc gtccagattg acgcgcatcg
     6721 tctcgcgcca aacttcggga tcctgctccc agacgacccg ctcatgggcc gagccgatgc
     6781 catggttcac gacgagcacg tcgatcggcc ccaaccgtcg ctccgtttca ccgacggctt
     6841 cggcgcagcc cgcggccgtg ccgaggtcgg cgacaacgta gtcaagtccc acgccggcga
     6901 gctcagccgc ggtgcgcgcg acgatcatca cgcgcattcc ggctgccgcc agcaaaagcg
     6961 cggcctcgcg tccgattccc cggccgccgc ccgttaccat cgccacacgc tgctgcaaat
     7021 cggctccgaa ggtgctgcga aatcctcttg aaccggcagt ttaacccaga gcgaggttta
     7081 acccttggcc gcgtcaatcg caacccccta gggcaccggc aacgagtccc ccatctcggt
     7141 cttcaagcgt gtccgccagt ccttgagact ctccgccggc acctggtcgc gaaggtcccg
     7201 atcttcacga gggtcgacgc tcagatcgaa tacccggtcc gcccccgact gctgatccaa
     7261 cgagtatttc caaacatgtg cggc
//
LOCUS       DASWZT010000551         1531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_62487, whole genome shotgun sequence.
ACCESSION   DASWZT010000551 DASWZT010000000
VERSION     DASWZT010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62487"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(391..1341)
                     /locus_tag="VGE92_15675"
     CDS             complement(391..1341)
                     /locus_tag="VGE92_15675"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY1315321.1"
                     /translation="MTQNSMVTAGIDTSKLKLDIAIHQRPERWQLPNSLPGWRELVIH
                     LAKAGVTRVGIEATGGYERGVVDHLRAAGFTVLVLQPIQVKAFGRVHLRRAKNDDLDA
                     VLIAACAAALDPPKIAPDPRLAELADELTFLEQIEEDIARFKIRLEHIAEPRLRRMVD
                     GDIARLKLRRLAQIRRLAKHLRDHDDLGRRFDLVLSIPGIGERTALALIIRMPELGRI
                     SREEAAALAGLAPFDNDSGQYKGQRRIAGGRGRLRKSLFAAALPAAFQWNTALTALYA
                     RLTAKGKKHNVALIACARKLLIFANTVVQRGTPWVESTAS"
BASE COUNT          299 a          475 c          480 g          277 t
ORIGIN      
        1 cagcggtgct ataccccggc tggacaccag atggacactt gaaaactaac gtgttgaaat
       61 aactatcaaa tatcgtctgg tgggacaccc acaagggtcg cccctacaaa tgaacagcgg
      121 tggcacgcca tcacggtgag ggtcgtcagc tctatccgcg tagcaaccat tataaaacgc
      181 ccattcgaaa aaccctgcaa atcgccctga cccggccagc atgacgccgt gctcgccgcc
      241 atcaaggagt ggtctggcga agacggctta tgacgcgccg gcacggcgac gaccatcctt
      301 gacggcgtct gcgcgcgtcg tcatggggca agcaggtcgg gacgaagaaa cggcgctgcg
      361 gtcgaaccaa gaaactccca aggcgtggct tcagctcgcg gtactttcga cccacggcgt
      421 gccgcgctga accacggtgt tggcgaagat caaaagcttt cgcgcgcagg cgatgagcgc
      481 cacattgtgc tttttgcctt tggcggtcag gcgagcatag agcgccgtca gggccgtgtt
      541 ccactgaaag gcggctggca gggctgccgc gaacagggac ttgcgcaagc ggccccggcc
      601 accggcgatg cggcgctggc ccttgtattg tccgctgtcg ttgtcgaacg gcgcgagccc
      661 ggccagggcg gcggcttcct cgcggctgat ccgtccaagc tccggcatgc ggatgatcag
      721 cgccaacgcg gtacgctcgc cgatgccggg gatgctcagc accaggtcga accgcctgcc
      781 gagatcgtcg tggtcacgca gatgtttggc gagcctgcgg atctgagcca ggcgtcggag
      841 cttgagccga gcaatatcgc catcaaccat gcgccgcagc ctcggttcag cgatgtgctc
      901 gagccggatc ttgaagcgcg cgatgtcttc ctcgatctgt tcgaggaagg tcagttcatc
      961 ggccagttca gccagcctcg gatctggcgc gatcttcggc ggatcgagcg cagctgcgca
     1021 ggccgcgatc agcaccgcat cgaggtcatc gttcttggcc cggcgcagat gcacccggcc
     1081 aaaggccttg acctggatcg gctgcaacac cagcacggtg aagcctgccg cccgcagatg
     1141 atccacgacg ccgcgctcat agccccccgt cgcctcgatg ccgacgcggg taacgccggc
     1201 cttggccagg tggatgacga gctcgcgcca gccaggcaag ctattgggga gctgccaacg
     1261 ctcgggtcgt tggtgaatgg cgatgtcgag tttgagtttc gaggtatcga ttcctgctgt
     1321 aaccatgcta ttctgtgtca tcttcgtcga ccctgccttg taaagcgaac caagttgttc
     1381 cggcaaccat ccgggtccga atgaagagct gacgcgatca cgctacgagg cagccagaag
     1441 cggctcaggg cgggtacgat cagatcgcca gcggcctgtc gcggattgcg gccgcggcag
     1501 gccattcctc tcggaataga tcgattataa c
//
LOCUS       DASWZT010000552         1988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_76958, whole genome shotgun sequence.
ACCESSION   DASWZT010000552 DASWZT010000000
VERSION     DASWZT010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76958"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            346..513
                     /locus_tag="VGE92_15680"
     CDS             346..513
                     /locus_tag="VGE92_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315322.1"
                     /translation="MARLAAASNQIADNAATTNGAAPTKIKKEMVTFVYAIAAAITHG
                     IEKSAPVIPAK"
     gene            complement(689..1144)
                     /locus_tag="VGE92_15685"
     CDS             complement(689..1144)
                     /locus_tag="VGE92_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315323.1"
                     /translation="MTLCWPARGAELAMSALGSTLALLLLTTAVCAQPPTAPAAKSAA
                     ATLKRDTERCRTYSNLDACDDAIRWNPSDPALLVAQGDALQKAKRPADAIRAYRRAAA
                     LAPNLGGIDARIGAAQAKLSAARAPRARPAPVAAAPKRYSNAAPESQSH"
     gene            complement(1141..>1988)
                     /locus_tag="VGE92_15690"
     CDS             complement(1141..>1988)
                     /locus_tag="VGE92_15690"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY1315324.1"
                     /translation="AAPPAAGAVIRDCPTCPALTVLPAGRFQQGSEAVADGSAPFEKP
                     LHWVAIAQAFAMSTNAVTVDEFRAFISMTGRDMRGCDIYDGEWRPKPENNWENPGFVQ
                     TGSHPVTCASWEDAQAYAGWLSAKTGHRYRLPSASEWEYAARAGGAAVQPWDSSGAGA
                     CANANVADASAAREFPGWTVFTCDDGYVNTAPVGSFKSNAFGLNDMLGNVFQWTEDCW
                     HTDYIGAPVDGRARTDGDCSQRELRGGSWFSTPAYVRANYRNHFAANYRTSSVGIRLV
                     RDLAS"
BASE COUNT          406 a          611 c          618 g          353 t
ORIGIN      
        1 gcaccagcaa atgcggattt cattagcgtc caccgatttt agcgtaatcc acatgtttag
       61 ttttgggctg gcctggtgat tattttcggt gggatatatc tatacccccc aaccgaatat
      121 tagagggcaa aacgtctcgt actggcgatc aagcccagca ctacgccacc gatcaaaagg
      181 agcggccaaa acacaagcaa tgaacccacg gcatatatga gaaacgctcc aagcagagag
      241 gctccgcgga caattgcgag caacaaagag tagagcgcaa cgccgctgag gtacgtcatt
      301 gtcgcaccga cacacccggc aatcgcgagt ccactgcgtg acgctgtggc gcgccttgct
      361 gccgcgtcga accagatagc ggacaatgcg gccaccacca acggagctgc gccgacgaaa
      421 atcaagaaag agatggtgac atttgtatat gcgattgccg cagcgattac gcacgggatt
      481 gaaaaaagtg cgcccgttat tcccgccaaa tagcatcgct tacgccgagg tgccatggtc
      541 cctctgactg tcaatcagga ggccgatttg tcgtataacg gcgggagtgt cgcgatacac
      601 cagattctct ccttgaaccc attggcttgc atcaatagta cggcggagcc gcaagaaagg
      661 aaatgtgacg gtggtggcat gacgccggtc agtgcgactg cgactccggg gctgcattcg
      721 aatagcgctt cggcgccgcg gcaaccggcg ccggccgcgc acgcggcgcg cgtgctgcgg
      781 agagcttcgc ctgcgccgcg ccgatccggg catcgatgcc gcccaagttg ggtgcgagcg
      841 cggcggcgcg tcgataggcg cggatcgcat ccgccgggcg cttggccttc tgcaacgcgt
      901 cgccctgcgc gaccagcagc gccggatcgc tgggattcca acgaatcgcg tcgtcacaag
      961 cgtcgagatt cgaataggtg cggcagcgct cggtgtcacg cttgagcgtc gctgccgcgg
     1021 acttggccgc gggggctgtc ggtggctgcg cgcacacggc tgtcgtcagc agcagcaatg
     1081 caagcgtgga tcccagggcg gacatcgcta gttccgcgcc gcgcgcgggc cagcacaacg
     1141 tcacgatgcc aagtctctga ccagtcgaat tcctacactg ctggtgcggt agtttgcagc
     1201 gaagtggttt cggtagttag ctcgcacata ggcgggtgtg ctgaaccagg atccgccgcg
     1261 aagctcgcgc tgcgagcaat cgccgtcggt gcgtgccctg ccgtcaaccg gggcgccgat
     1321 ataatctgtg tgccaacaat cctccgtcca ttgaaaaacg ttgcctagca tgtcgttgag
     1381 gccgaaggcg ttggatttga aggaaccgac gggagccgta ttgacgtagc catcgtcgca
     1441 ggtgaatacc gtccagccgg gaaactcgcg tgccgcgctc gcgtcggcca cattggcgtt
     1501 ggcacaagcg cccgcgccgc tcgaatccca cggttgaacg gccgcgccgc cggcgcgtgc
     1561 ggcatattcc cattccgaag cactcggcaa ccgatagcga tgcccggttt tcgccgataa
     1621 ccacccggca taggcctgcg catcttccca cgaggcacac gtgaccggat gcgatccggt
     1681 ctgcacgaag ccgggatttt cccaattgtt ctcgggcttc gggcgccact cgccgtcgta
     1741 gatgtcacag ccgcgcatgt cccgcccggt catcgagatg aaggcacgaa attcgtccac
     1801 ggtgacagcg ttggtcgaca tcgcgaacgc ctgcgcgatc gccacccagt gcagcggctt
     1861 ctcgaagggc gcggaaccat ccgcaacggc ctcggagcct tgttgaaatc gacccgcggg
     1921 cagcaccgtg agggcgggac atgtggggca atcacggatg acggcaccgg ctgcgggcgg
     1981 agccgcca
//
LOCUS       DASWZT010000553        22999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_86653, whole genome shotgun sequence.
ACCESSION   DASWZT010000553 DASWZT010000000
VERSION     DASWZT010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 22999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22999
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86653"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VGE92_15695"
     CDS             <1..286
                     /locus_tag="VGE92_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005108577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HEY1315325.1"
                     /translation="RKDHLFGERERQFQLYRRGRYVEFNLVYDRGTLFGLQSRGRTES
                     ILMSMPPLARWDYDWHPEPGSAEARLYDYLRPRAYLSELLTTRQWPSANT"
     gene            complement(262..618)
                     /locus_tag="VGE92_15700"
     CDS             complement(262..618)
                     /locus_tag="VGE92_15700"
                     /inference="COORDINATES: protein motif:HMM:NF018008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY1315326.1"
                     /translation="MANRLLPLRDLTAPMETDAPWPGTALSGDPHTKTWNLHTSDDGK
                     ILAGIWESTPGVWQIDYDAWEYCHLLAGRCTIEIEGKPPVTLKSGDVFIVESGAKGKW
                     TVHENLRKYYVFALGH"
     gene            726..1955
                     /locus_tag="VGE92_15705"
     CDS             726..1955
                     /locus_tag="VGE92_15705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY1315327.1"
                     /translation="MDDKSALLNQLRIDRAAAPAPTARWPWLALAAIILLAALGIWWA
                     TRPSAVPVHVVAPQPIAGGSSAAAGSILDASGYVVARRQATVASKITAKMVELDIEEG
                     DRVKAGQIIAKLDDTNVRAALDQTRAQLDYAKASLTETEVNLANAQRDYDRQKSLLQA
                     HYVSQAAVDNSQTTMDALRAQLATQRSNVEVVARAMNSAERNLDDTIVRAPFSGIVTV
                     KAAQPGEMVSPVSAGGGFTRTGIGTIVDMDSLEIQVDVNENFINRVRPAQQVTAKLNA
                     YPDWQIPAHVIAVIPTADRSKGTVTVRIALDQKDARILPEMGVRVSFLADTSQPPAGQ
                     TAAPGVILPANAVQGSGTTGTIFVVHDDTVERRAVRLGGTSGDGVVVLSGLTAADHVA
                     VGDFAQLKDGARIRVEQ"
     gene            1968..2660
                     /locus_tag="VGE92_15710"
     CDS             1968..2660
                     /locus_tag="VGE92_15710"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1315328.1"
                     /translation="MSEAIVRLRNVDKRYTRGKQSVEVLQGLSLDIPQGDFVALMGPS
                     GSGKTTLLNLIGGLDKPSSGEITVAGQRLDQLSSGGLAHWRANHVGFVFQFYNLMPML
                     SAQRNVELPLLLTHLSAAQRKANALAALTLVGIADRAKHKPNELSGGQAQRVAIARAL
                     VADPTLLVCDEPTGDLDRKSADDVLHLLQVLNREHGKSIVMVTHDPKAAEFAGRRLHM
                     DKGALLEAGTMA"
     gene            2677..3843
                     /locus_tag="VGE92_15715"
     CDS             2677..3843
                     /locus_tag="VGE92_15715"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY1315329.1"
                     /translation="MKYFPLVWATLWRKKTRTIFTLLSVVVAFLLFGVLETVDYAFAH
                     PSSGVTGADKLLTMNKYSITLSLPFSDVQEIRSVPGVAEATWITWFGAYYQDPKNFVF
                     ALPIDTDSYFNLHKGEFSVAAAQMQAFRNTRSGALVNSALLQKFGWKIGDRLPLHSTI
                     WTQKSDDSLDWKFDIVGTFAVKDATQASAQSSTVLFHHEMFEEGRSFGKGTVGWIEER
                     VQDPAQAAQIARQIDTLFANSPNETKTQPANDFAMTFIKQFGDIGFVLRAILGAVFFT
                     LLFLTGNTMMQSVRERIPELAVLKTLGFGDGHVLGLVLAESLLLCVIAALLGLALSYA
                     ALPVIKQGLQGVELSPRALIPGLGVAVLLALIVGMPPALRAMKLNIVDALADKR"
     gene            3868..5046
                     /locus_tag="VGE92_15720"
     CDS             3868..5046
                     /locus_tag="VGE92_15720"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1315330.1"
                     /translation="MLKQLTAVISMSLQTLPQRVGASSVIVIGIAGVVAVLVSVLAMG
                     AGFRHTLADSGRADRAIILRGGSDAELNSNLTRTDVATISNAPGLLQDSSGKAVLSSE
                     LVTVVNVPKIDTGTDANVTLRGVGLKLTEVRPELKIVAGRMFRPAVRELIAGAGAAKQ
                     FRGLTPGAALHLRNADWTVTGVFTSNGDVHESELLADVDTVGSSIERNGYSSAIGLLQ
                     SAADFAAFRDALTTDPQLKVDVKREPDYYAAQSKQLSKTINVVGNTVAVIMAIGAMFG
                     ALNSMYSAVAARGLEIATLRAIGFGALPVLLSVMIEALLLSLLGGVIGASLAWVFFNG
                     HSVSTLGGAFAQVVFQLTVTRTLIITGIVWACVIGLLGGFFPALRAARLPVAEALRAA
                     "
     gene            5058..5906
                     /gene="rlmJ"
                     /locus_tag="VGE92_15725"
     CDS             5058..5906
                     /gene="rlmJ"
                     /locus_tag="VGE92_15725"
                     /inference="COORDINATES: protein motif:HMM:NF016278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEY1315331.1"
                     /translation="MNYRHIYHAGNFADLAKHVGLLYCMSALQRKEGALFVLDSHAGR
                     GYYDLQAPEARTSGEADRGVQRLIETRLDATVLADYFAAIGARRGKRLSLYPGSPALI
                     AKRLRPQDRALFVEVLPAEARAIEREIDSVGRVRTAIGDGYAALKAHLPPEERRGLVL
                     IDPPYESLDELKLMLLAFTEAYRRWRSGVFLIWYPIRSATQRSHVHARFASLGIPKML
                     FADLAIHPDDAGVGLAGSGLIIINPPYGTDAFLADAYGEIHRGIAQPGAGYVQVARLT
                     PERMPQ"
     gene            5921..6229
                     /locus_tag="VGE92_15730"
     CDS             5921..6229
                     /locus_tag="VGE92_15730"
                     /inference="COORDINATES: protein motif:HMM:NF025715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HEY1315332.1"
                     /translation="MEKDSLHALAAKLHAELSEALRADPKSRESLSEMMRDINRLIDK
                     PAVSPVPAPVPAPASPPGAWDRSLPDRLEKIAVQFEVNHPTLAASARRLVDLLGEVGI
                     "
     gene            complement(6231..6938)
                     /locus_tag="VGE92_15735"
     CDS             complement(6231..6938)
                     /locus_tag="VGE92_15735"
                     /inference="COORDINATES: protein motif:HMM:NF023287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3379 family protein"
                     /protein_id="HEY1315333.1"
                     /translation="MDCATYRRTLMADPHDPDPTLREHRSGCEECNLYTDRLLRFESR
                     LERALRVPQAGPTAAPGIATPPLRAPAYRRRWLAMAASILVAATVAGGLWLGVPRSSL
                     AADVVTHMREEPQAWRRTDVPVPTSALQGVLDESHLRLSNDAGLVSYANSCGFRGHQV
                     PHLVLQTASGPVTVMVLVHEAAKKIEPFEERGYRGVIVPVAGHGSLAVLTQGTTTDIK
                     TIERIASRVQDSIVWTS"
     gene            complement(6938..7465)
                     /locus_tag="VGE92_15740"
     CDS             complement(6938..7465)
                     /locus_tag="VGE92_15740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY1315334.1"
                     /translation="MKDQVGGNQKRARFQALCQSLRPDLLRFAFWLSRDRALAEDVVQ
                     ESMLRAWKAQDALQDEAAAKPWFLTIIRREYARSFERKRFVTVDVDELVAREEPTLAV
                     AEEQDLAELRAALFKLPDDYREPLVLQVLMGFTTAEIARELDLSCAAVLTRLFRARKQ
                     LRAICGEDTRLDPQE"
     gene            7607..8614
                     /gene="hemB"
                     /locus_tag="VGE92_15745"
     CDS             7607..8614
                     /gene="hemB"
                     /locus_tag="VGE92_15745"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005179750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEY1315335.1"
                     /translation="MTGNTANSFPSTRLRRMRRDEFSRRLMRESTLGVDALIYPVFVV
                     EGRNQRQPVASMPGVERLSIDQLLRECETLLALKIPAIALFPVTPQEKKTLDARESWN
                     PNGIAQTAVRAVKKEFPGLGVITDVALDPFTTHGQDGLIDDAGYVLNDVSVETLVRQA
                     RSHADAGADVVAPSDMMDGRIAAIRAAFEGAGLANTRILAYSAKYASSFYGPFREAVG
                     SAGQLGKGGKHTYQMDPGNSNEALREVALDIEEGADMVMIKPGLPYLDIVRRVKDRFG
                     MPTFVYQVSGEYAMIMAAAQAGWVDERAVALETLLSMRRAGADGILTYFAKRAAEWLA
                     E"
     gene            8623..9456
                     /gene="aroE"
                     /locus_tag="VGE92_15750"
     CDS             8623..9456
                     /gene="aroE"
                     /locus_tag="VGE92_15750"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001310.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEY1315336.1"
                     /translation="MGTDAPDQYGVVGHPIAHSWSPFIHGMFAKSTAQNLVYRLFDVP
                     ADNFRREALRLFASGVRGLNVTLPHKQAAAELVNELTPRAERAQAVNTIAFFEDTSLL
                     GDNTDGLGLTADLERNLNINLADKRVLILGAGGAVRGVLGPLLERELAEVIIANRTPD
                     RAHKLATEFADMGQISGCAFAEIRGPSYDIVINATSAGLQGEMPELPAGLVGARTICY
                     DMAYGRGHTPFTLWAKSLHAARVSKGWGMLVEQAAESFLLWRGMRPDTRPVLQALSQH
                     A"
     gene            complement(9465..10814)
                     /gene="gor"
                     /locus_tag="VGE92_15755"
     CDS             complement(9465..10814)
                     /gene="gor"
                     /locus_tag="VGE92_15755"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002122354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEY1315337.1"
                     /translation="MSDQAFDLISIGGGSGGLACAQRAAEFGAKALVIESHRLGGTCV
                     NVGCVPKKVMWNAANVALSLADAQDYGFSVTAGGSDWPMLKAKRDAYVLRLNGIYERN
                     LATKGVTYVRGTARFLDKQALEVNGMRLTAPHIVIATGGRAIVPMLPGAEHGMTSDGF
                     FSLPQRPARVAVVGSGYIACELAGAFHELGSQVELFIRKDHLLTSFDAMLGKSLMREM
                     QAQGITIHPRVIPASLREDSGLQTLVAHGGTEFAGFDCLLWAVGRAPNVQALDLQNAG
                     VAVDDGNFVITDAFQNTNVAGVYAIGDVTGRAQLTPVAIAAGRRLSDRLFGGKVDRRL
                     EYSMIPTVVFTHPPIGTVGASEAEARAQYGDAVRVYVADFTPMYHALTARKTRTDMKL
                     VCVGAEQRIVGCHIIGAGADEMLQGFAVAIRMGATKRDFDDTVAIHPTSAEELVTMR"
     gene            10892..11464
                     /locus_tag="VGE92_15760"
     CDS             10892..11464
                     /locus_tag="VGE92_15760"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY1315338.1"
                     /translation="MIGVGVTLALTAALLVYLVGIYNGLVGLKENVKTAWANIDVLLK
                     QRHDELPKLVETCKRYMQFEQETLEKVMRARASVGQASSSGNIAAVGAAEQQLRAGVG
                     RLFAVAESYPQLKSDQTFKQLQGRITSLEESIADRRELYNDQVNLNNIRVQVFPDVMI
                     AHLGGFLPAQLLEFSSDEKRDVDVGALFGT"
     gene            11480..12370
                     /locus_tag="VGE92_15765"
     CDS             11480..12370
                     /locus_tag="VGE92_15765"
                     /inference="COORDINATES: protein motif:HMM:NF023899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006996 - organelle organization [Evidence
                     IEA];
                     GO_process: GO:0016567 - protein ubiquitination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIDE domain-containing protein"
                     /protein_id="HEY1315339.1"
                     /translation="MSDDAIEYWFILISLAAGSGYSFWYAFSSWRKNRTICDLPVSRV
                     RSAAQGYVQMDGIGMMPPGSANQSPLTRTPCTWWSYEVEKRDSEGWCTIDSETSAAPF
                     LLDDGTGQCLIDPRGAEVIPRATHVWYGSEAWPRMRLPEGQGIFGRLIDRIFSGRYRY
                     TERRLQPRELVCAVGAYRSVGGREDADLAAAELLREWKRDQAALLARFDSDRDGRLSE
                     REWNRARDAAQREVADSRAAAPIPPTVNMLLDPMDGRAFVLAARDAAALARGLRGRAL
                     LGITGFVGGSAAVAWMLSLL"
     gene            12384..14402
                     /locus_tag="VGE92_15770"
     CDS             12384..14402
                     /locus_tag="VGE92_15770"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY1315340.1"
                     /translation="MSNPLLELDSLPAFGRIEARHARPALLKVLAENRARLSELTAQA
                     QPTFASLVIPVEELSYRLSRVWSPIGHLNAVANSTAMREAYNECVPLLTAYSSELGQN
                     AELQAGYAYVLQHEGKALDPPQRKVVENALRDFRLAGVDLGPEQKTRYREVAQRLAQL
                     ATKFSENVLDAGRAYTRSVTDGAQLAGLPANAVDRAAADAREANQSGWLFKLDQPTYM
                     TLMASAESADLRRDIYEAWVTRASEVGPSAGRFDNSAIIAEILPLRHELARLVGFENF
                     ADYALATRMAKSSKQVLGFLDDLARRCLPAARQEFADLEEFAGRKLNAWDIAFYSERL
                     QERRFKVSQEALRPYFPLPKVLEGLFSLLRRLYGISVRERPGVGVWHSSVRYYDLLDR
                     DGKIVAGFFLDPYSRSEKRSGAWMDECVIAKSLPDGTALPVAQLVCNFTAPVGNAPSL
                     LTHDEVTTLFHEFGHGLHHMLTRVAYPSIAGINGVAWDAVELPSQFMENFVWRAEVLP
                     LISAHVDSGETLPVEMLQRLLGTRTFNAALDTLRQIELAAFDFELHANFDLELGPRVA
                     ETLAGVRKRVAVVPAAPFNRMPASFAHIFAGGYAAGYYSYKWAEVLAADAFEAFAEAG
                     VFDSATAARFRDSILARGGSLDAMDAFVRFRGREPDVRPLLKQTGIAA"
     gene            14399..14989
                     /locus_tag="VGE92_15775"
     CDS             14399..14989
                     /locus_tag="VGE92_15775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HEY1315341.1"
                     /translation="MKCPFVCFGALLTARLAAAPAYVSDELVLGVYAEQNSQGQRLAT
                     LHSGASLETLSVSGEFTQVRLNDGTTGWVKSTYLTTQEPASARVKQLEEELDRRHATT
                     PELAEAAARSELEQLKRQLASQQSGLEAAKAAQPQAAAPANPPAASGAPGVGARLSHL
                     GWAVGAVLAALAVGFWLGYATLARRVRHKFGGLKVY"
     gene            15103..16098
                     /locus_tag="VGE92_15780"
     CDS             15103..16098
                     /locus_tag="VGE92_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315342.1"
                     /translation="MHDLRRNDYDVTNSVQVSSTAAVTAAVRELFTSAFPGESFDRVG
                     VSFEEFDKLFTGRMPGYHGVDTVYHDRQHSLDMTLAMARLLVGYERTVEPRMRFGAER
                     AVMALVTSLFHDAGYIREFGDRQHRNGAEFTLYHVTRSARFLARFLPTIGMEDWVPVS
                     TRIVHFTGYEIKLDQIQLADPRDRKLGHLLGTADLIAQMADRCYLEKCRDRLYPEFVL
                     GGIAAATGTDGALQVRYSSGLDLLRQTPEFVRETRQLRLEGEFEHAYRYIEPLFGGKN
                     PYLEAIDRNLVFLHRVLRNERWLMLKRNPPLFTSGDNQLGQVRGLMLTHLKALWA"
     gene            complement(16095..16769)
                     /locus_tag="VGE92_15785"
     CDS             complement(16095..16769)
                     /locus_tag="VGE92_15785"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEY1315343.1"
                     /translation="MNDSELTGQSRSHITEVPDPTCALHAQVVVPFQNLRRAALADGF
                     DVRPQSSFRDFSRQLTIWNGKFSGARPMYDAQGGRIEVSQLSAAERVAAILLWSALPG
                     ASRHHWGTDVDLVDARATAPGYQVQLTPQEFATGGPFAALAEWLEANAARFGFFRPFR
                     GLLSGVQPEPWHFSFAPVAESARRSLSPAVLHRAIAAAPLSGKDEVLARLPELHARYV
                     AAIDWP"
     gene            complement(16766..17416)
                     /locus_tag="VGE92_15790"
     CDS             complement(16766..17416)
                     /locus_tag="VGE92_15790"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEY1315344.1"
                     /translation="MRIDIYSDTICPWCYIGKRRFELAVAARPQYETRVFWRPFELNP
                     DLPIEGVDRARYLAARIGPPERIAAAHAVLKSQGEASGIDFRFDLMARVPNTRRSHLL
                     IAHAARSGRQAAVKERVMHAYFEEGCDIGEVEVLVRLGVEAGLSERESRAALILRAGQ
                     DGVLAAERHAAVLGITGVPTFVFDGQYTISGAQEVGTLTRIFDQVADFAAERDLAS"
     gene            17521..19998
                     /locus_tag="VGE92_15795"
     CDS             17521..19998
                     /locus_tag="VGE92_15795"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY1315345.1"
                     /translation="MARSLKTKYLLLALAVGAALSLLLGGLSYYEHRIDAADVNQITY
                     ATVAQKLEADLETRASSLSEITGTSLAAAIGSGNKAAVASIAERLLDERDIDRVEVSD
                     AHGTVLFIGGDAAPRQAGDWFAFQSTIHSNLVAAPVQQVGSLRIWMSRAEMRETLASI
                     RKQLESQQGMQIKRMRGLLAGITLPLLVLGLAGAWFIARQLAGPISALVKTADRIGQG
                     DYTRPLAVVRHDELGDLQFALERMRQKLNETTITKNYLNTVLNSLSDAVLVTSPEGIV
                     KSCNEATQRLLGYQEDELVGKPLVSFIDEVHRNAFDLSNSAPEARETVLRTASGQTIP
                     VSMASSAITTEDPQFQGNIYVARNITERKRAERRIRYLARYDTLTKMPNRMQFQHLLQ
                     QAIARARRRQRSLALLYLDLDRFKEVNDTFGHAAGDRTLEILSERLTRNLSKDTVIGR
                     LAGDEFAMFIDDLPMDVDNRAGIGTLARTLLDEISRTFYVNQQEVYLTASVGVAICPY
                     DAENVIDLIRNADAAMYHSKQNGGNSCAFYVPEMNAAAVERLMLKSKLRRALERDELV
                     ILYQSKVDLRNGRVVGAEALLRWRLPGHGDISPSQFIPLAEETSLILDIGEWVLNRVC
                     SDYREWQQTVPEPGRIAINLSLRQLKQSTFIARCRAVFRKHEVSPTCFELEVTETTLM
                     TDPKRTIGLLNELYAMGLNLAIDDFGTGYSSLSALQQFPIGTLKIDQSFVRDVAVDSD
                     DAAIVRTIIDMGRSLDLEVIAEGVEAGEQLEFLRKHGCYYGQGRLFGDAMEASKMLAI
                     LAGQAAGTAHHASLCVPTLVRPIRLTS"
     gene            20008..21885
                     /locus_tag="VGE92_15800"
     CDS             20008..21885
                     /locus_tag="VGE92_15800"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1315346.1"
                     /translation="MLSARDLTLRRGPQPLFAEVNFSVYRGDKVGITGANGTGKSSLF
                     AAIQGELAADRGDIDRPANLKIAHVEQEIAAGAERNAIEFVLGGDVELCNVLAAIADA
                     ERRDAPLALAESHAALLAIDGYRARARAAAIMHGLGFKASDHERSVAEFSGGWRVRLA
                     MARALNSRADLLLLDEPTNHLDLDAIVWLEDWLTAFKGTLLMISHDREFLDAIIDRVL
                     HIENHAVRGYSGNYSQFEQKRAEELALQQVLEARQLRRVAQITAFVNRFRASASKSRQ
                     AQSRIKMLERMERIVPAHVDSPFEFEFAAPRGTPRPLLTVEGAACGYGDTPIVAGIDA
                     SIGPQDRIALLGPNGAGKSTLTRMLAGELPPLAGTRVAAADVCIGYFAQQQLQQLKPD
                     CDAFWHLRNLGGVDFAQGDEQRVRDHLGRFGFAGERAFEPVGRFSGGEKARLTLALVV
                     ARRPNLLLLDEPTNHLDIDMRHALTVALQSFEGGMLLVSHDRHLIKTVADSLWLVADG
                     KLKVFDGDLDDYQDWLRSRGAPKPARRAPESPAKAAAPATPRARLSRLHRESEQIELR
                     LTAIAAERDRLEAQLCQDPMHRGLQAQHADLTRDAAAMEVRWLEVGMAIEAAQAGIDT
                     E"
     gene            complement(21887..22519)
                     /locus_tag="VGE92_15805"
     CDS             complement(21887..22519)
                     /locus_tag="VGE92_15805"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEY1315347.1"
                     /translation="MSRTIRRAVILAAACLSACSGLFQSKIAPPTIYVLAAAPPAQAP
                     PDARVPVDLAILKPKMPAGLQMDRIAVLYPDRRLDYFADARWSGPLTEVLQDFAVREF
                     QLHGNLRTVSGDTSVFASSYWLELEFSDFQAEYTQASAAPAVHVRLLARLGRSGDRRI
                     LGRFEADALKPAAQNRLTAIVDAYAQAADAALGEIVAAAAAVLAKNSQSP"
     gene            complement(22516..>22999)
                     /locus_tag="VGE92_15810"
     CDS             complement(22516..>22999)
                     /locus_tag="VGE92_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315348.1"
                     /translation="TTHLVELADRFNQVFSDDNVRSFNTTLTNVRLASEHLPGTFREV
                     QELVADVRRMTQEVQGAASDLRRIETHATPDIESAVANIRQVSDNLAKTSERLQQFIA
                     DNEPGVSRFTQQSLPEFEQLLRESRQAARDFRDLSRSLKQDPSQLIYEPNYRGVEVAK
                     "
BASE COUNT         4165 a         7342 c         7339 g         4153 t
ORIGIN      
        1 gcgcaaggat catcttttcg gcgaacgcga gcgccagttt cagttgtacc gccgcggccg
       61 ctacgtcgaa ttcaatttgg tttacgatcg cggaaccttg ttcggcctgc agtcccgcgg
      121 ccgcaccgaa tccattctga tgtcgatgcc gccgctcgca cgctgggact acgattggca
      181 tcccgaaccc ggatcagcgg aagctcgctt gtacgactac ctgcgcccga gggcctacct
      241 gtccgaactt ctgacgaccc gtcagtggcc gagcgcgaac acgtagtact tgcgcaagtt
      301 ctcgtggacc gtccattttc ctttggcgcc ggactcgacg atgaacacat cgccgctctt
      361 gagcgtcacc ggcggcttgc cctcgatctc gatcgtgcaa cgacccgcga gcagatggca
      421 gtactcccat gcgtcatagt cgatctgcca cacgcccggc gtcgattccc aaattccggc
      481 gagaatcttg ccgtcgtccg aagtgtgcag attccaggtc ttggtgtgcg gatcgccgct
      541 cagcgcggtt cccggccagg gcgcgtccgt ttccatcggc gccgtcaaat cgcgcagcgg
      601 cagcaatcga ttagccatga atttaccctt ctggtttgtc aaatggcggc cacggtagcg
      661 cctcgcgccg cgcgcggcaa tcgattggcg tcgcagcgcg cttttatgtt ttcatgccac
      721 gatacatgga cgacaaatcc gccctgctca atcaactgcg catcgatcgc gccgccgcgc
      781 cggcgccaac cgcccgctgg ccctggctgg cgctggcggc gatcatactg ctggcggcgc
      841 tcggaatctg gtgggcgacg cggccgagcg ccgtgcccgt gcatgtggtc gcgccccagc
      901 cgattgccgg cggcagctcg gccgcggcgg gctccatcct cgatgcctcg ggatacgtcg
      961 tggcgcgccg tcaggccacg gtcgcctcca agatcaccgc caagatggtg gaactcgata
     1021 tcgaagaggg cgatcgcgtc aaagccggcc agatcattgc caagctcgac gataccaatg
     1081 tccgtgcggc gttggatcag actcgcgcgc agctcgacta tgcgaaggcc agtctgactg
     1141 aaaccgaggt caatcttgcc aacgcgcagc gcgattacga ccggcaaaaa agcttgctgc
     1201 aggcgcacta cgtcagtcag gcggcggtgg acaattcgca aaccaccatg gacgcgctgc
     1261 gcgcgcagct agccacccaa cgcagcaacg tcgaggtggt tgctcgcgcc atgaattcag
     1321 ccgagcgcaa tctcgacgac accatcgtgc gcgcgccgtt ctccggcatc gtcacggtca
     1381 aggccgcgca accgggcgag atggtgtcgc cggtctccgc gggcggcggc ttcacgcgca
     1441 ccggaattgg tacgatcgtc gacatggatt cgctcgagat ccaagtcgac gtgaacgaga
     1501 acttcatcaa ccgcgtgcgg cccgcccaac aggtgaccgc caaactcaat gcctatccgg
     1561 actggcagat acccgcgcac gtgatcgcgg tgattccgac cgcggaccgc agcaagggca
     1621 cggtgacggt gcgcatcgcg ctcgatcaaa aggacgcgcg catcctgccc gagatgggtg
     1681 tgcgcgtctc gtttctggcc gatacatcgc aacccccggc cggccaaacg gcggcacccg
     1741 gtgttatcct gccggccaac gcggttcaag gatccggcac gaccggcacg attttcgtgg
     1801 tgcacgacga taccgtggaa cggcgcgcgg tacgcctcgg cggcacgagc ggcgacgggg
     1861 tggtcgtcct atcggggttg acggcggcag accatgttgc ggtcggagat ttcgcgcaac
     1921 ttaaggatgg cgccagaatt cgcgtcgaac aatgaagggt cgaagacatg agtgaagcta
     1981 tcgtgcgcct gcgcaacgtc gacaagcgct acacccgcgg caaacagagc gtcgaggttc
     2041 tgcaggggct ctccctggat atcccgcaag gcgacttcgt cgccctgatg ggaccgagcg
     2101 gctccggcaa gaccacgctc ttgaatctga tcggcgggct ggacaaaccc agctcgggtg
     2161 agattaccgt ggccggccag cgtctcgatc aactgagcag cggcggcttg gcgcactggc
     2221 gcgccaatca tgtcgggttc gtgttccaat tctacaattt gatgcccatg ctgagcgccc
     2281 agcgcaacgt ggaactgccg ctgctgctca cgcacttgtc cgctgcgcag cgcaaggcga
     2341 atgcgctcgc ggcgctcacc ttggtcggca ttgccgatcg cgccaagcac aagcccaacg
     2401 aattgtccgg cggacaggcg cagcgcgtgg cgatcgcacg cgccttggtt gccgatccga
     2461 cgctgttggt ttgcgacgaa cccacgggcg atctcgaccg caaatccgcc gacgatgtgt
     2521 tgcacctcct gcaagtgctc aatcgcgagc acggcaagag catcgtgatg gtcacccacg
     2581 acccgaaggc cgccgagttc gccggccgcc ggctgcacat ggacaaaggc gctttgctcg
     2641 aagccgggac catggcgtga ggcccgcggc gcttcgatga aatacttccc gctcgtatgg
     2701 gccactttat ggcggaaaaa gacccgcacc attttcaccc tgctgtcggt cgtggtggct
     2761 ttcttgctgt tcggggtgct ggaaacggtc gactatgcat tcgcgcaccc gagttccggc
     2821 gtcaccggtg ccgacaagct cctgaccatg aacaagtact cgatcactct gtcgctgccg
     2881 ttttccgacg tgcaagagat ccgctcggtg ccgggcgtcg cggaagcaac gtggatcacc
     2941 tggttcgggg cctattatca ggatccgaag aatttcgtgt tcgcgctgcc gatcgacacc
     3001 gacagctatt tcaatctgca caagggcgag ttcagcgtcg ctgccgcgca aatgcaggcg
     3061 ttccgcaata cccgcagcgg cgcgctggtc aactcggctc tgctgcagaa attcggctgg
     3121 aaaatcggcg atcggttgcc cctgcactcg accatttgga cgcagaagag cgacgactcg
     3181 ctcgactgga agttcgacat cgtcggcacc ttcgccgtca aagacgccac ccaagcgagc
     3241 gcgcaatcct cgaccgtgct gtttcatcac gagatgttcg aagaggggcg cagcttcggc
     3301 aagggaaccg tgggttggat cgaggaacgg gttcaagatc cggcgcaggc ggcgcaaatc
     3361 gccaggcaga tcgacacgtt gttcgcgaac tcgccgaatg agaccaagac tcaaccggca
     3421 aacgatttcg cgatgacctt catcaagcaa ttcggcgaca tcggtttcgt gctgcgcgca
     3481 atattgggcg cggtgttctt caccttgctg ttcctgaccg gcaacaccat gatgcaatcg
     3541 gtgcgcgagc ggattcccga gctcgcagtg ctcaaaacct tggggttcgg cgacggccat
     3601 gtgctcgggc tggtgctcgc cgagtcgctg ttgctgtgcg taatcgccgc gctgctcgga
     3661 ctcgccttga gttatgccgc gctgcccgtc atcaaacagg gattgcaagg ggtcgagctg
     3721 tcgccgcgtg cgctcatacc ggggctcggt gtcgcggtgt tgctggcgct gatcgtcggc
     3781 atgccgccgg cgctgcgcgc catgaagctc aatatcgtgg atgcgctggc ggacaagcgc
     3841 tgacgtgcac cgcggaggag tgcgaccatg ttgaagcagt tgaccgccgt catctcgatg
     3901 agcctgcaaa ctctgccgca gcgtgtgggc gcttccagcg tcatcgtcat cggaatcgcc
     3961 ggggtggtcg cggtgctggt ctcggtgctc gcgatgggtg cgggctttcg gcacaccttg
     4021 gcggacagcg ggcgcgcgga ccgcgcgatc attctgcgcg gcggatccga tgccgaactc
     4081 aacagcaatc tgactcgcac cgatgtcgct acgatcagca acgccccagg actgctccag
     4141 gacagcagcg gcaaggcagt gctgtcgagt gaactggtga cggtggtcaa cgttccgaaa
     4201 atcgataccg gcaccgatgc caacgtcacg ctgcgcggcg tcggcctgaa gctcaccgag
     4261 gtgcgtccgg agctgaaaat cgtcgccggc cgcatgttca ggccggcggt acgcgaactc
     4321 atcgccggcg cgggcgccgc caagcaattt cgcggactga cgcctggcgc cgcgctgcat
     4381 ctgcgcaatg ccgattggac cgtgaccgga gtttttacca gcaacggcga cgtgcatgag
     4441 tccgaactgc tcgccgacgt cgacaccgtg ggctcctcga tcgagcgcaa cggttatagc
     4501 tcggcgatcg ggctgctgca gagtgcggcg gatttcgccg ccttccgcga cgcgcttacc
     4561 accgacccgc agctcaaggt ggatgtgaag cgcgagccgg actactacgc cgcgcaatcc
     4621 aaacaattga gcaagaccat caatgtagtc ggcaataccg ttgcggtcat catggccatc
     4681 ggcgcgatgt tcggtgcgct caacagcatg tactccgcgg ttgcggcgcg cggactcgag
     4741 atcgccacct tgcgggcgat cggcttcggc gcgttgcccg tgctgctctc ggtcatgatc
     4801 gaggcgctgc tgctgtccct gttggggggc gtcatcggcg catcgctcgc atgggtgttc
     4861 ttcaatggac acagcgtcag cacgctgggc ggtgcctttg cgcaggtcgt cttccaactg
     4921 acggtgacgc gcacgctgat catcaccggc attgtctggg cctgcgtcat cggcttgctg
     4981 ggcggcttct ttcccgccct gcgggccgcg cggctgccgg tggcagaggc gttgcgtgcg
     5041 gcctgagtgc tgctcgaatg aactatcgcc acatctacca tgcgggaaat ttcgccgacc
     5101 tggcgaagca cgtcggcttg ctgtactgca tgagtgcatt gcagcgcaag gaaggcgcct
     5161 tgttcgtgct cgacagccac gcgggccgcg gctactacga tctgcaggcg ccggaagctc
     5221 gcacatcggg cgaggccgat cgcggcgttc agcgcctgat cgaaacccgc ctggacgcta
     5281 cggtgctcgc cgactatttc gcggcaattg gtgcgcggcg cggcaagcga ctaagccttt
     5341 acccgggttc gccggcgttg atcgcgaaac ggctgcggcc acaggaccgc gcgttattcg
     5401 tcgaggtgtt gccggcggaa gcgcgcgcca tcgagcgaga aatcgactcc gtgggccgtg
     5461 tgcgcaccgc catcggcgat ggctacgcgg ccctcaaggc gcatctgccg ccggaggaac
     5521 gtcgcggcct ggtattgatc gacccgccct atgaaagcct ggacgagttg aagctcatgc
     5581 tgctggcgtt caccgaggcc taccggcgct ggcggagcgg agtatttttg atttggtacc
     5641 cgatccgcag cgcgactcag cgaagccacg tacacgcgcg cttcgcatcc ttgggaatcc
     5701 cgaaaatgct gttcgccgac ctcgcgattc atccggacga cgcgggggtc gggctcgccg
     5761 gcagcggctt gatcatcatc aacccaccct acgggaccga tgcattcctc gccgacgctt
     5821 acggcgaaat ccatcgcggg atcgcccagc caggggcggg ctacgttcag gtcgcccgac
     5881 tgactcccga gcggatgcca caatagggca tactcgattc atggaaaaag actccttgca
     5941 cgcccttgcc gcgaagctgc acgccgaact ctcggaagcc ctgcgggccg acccgaagtc
     6001 gcgggaatcg ctgagcgaga tgatgcgcga catcaatcgc ctgatcgata agcctgccgt
     6061 ctcgcccgtg ccggcgcccg tgccggcgcc cgcttccccg ccgggcgcat gggatcgatc
     6121 gctgcccgat cgcttggaaa agatcgccgt tcaattcgaa gtcaatcacc cgaccttggc
     6181 ggcgagcgcc cggcggttgg tcgatttgct cggcgaggtg ggcatctgac ttaactggtc
     6241 cagacgatcg aatcctgcac ccgcgaagcg atgcgttcga tcgtcttgat atccgtcgtc
     6301 gtgccctggg tgagcacggc cagactgccg tggccggcaa ccggcacgat caccccccgg
     6361 tagccccgct cttcgaacgg ctcgatcttc ttcgcggcct cgtgcaccag caccatgacg
     6421 gtaaccggac ccgacgcggt ttggagcacc aagtgcggca cctgatgacc acgaaacccg
     6481 cagctgttgg catagctcac gagaccggca tcgttgctca ggcgcaggtg cgactcgtcg
     6541 agaacgccct gcagcgccga ggtgggcacg ggcacgtcgg tgcgccgcca tgcctgcggc
     6601 tcctcgcgca tgtgcgtcac cacgtctgcc gccaggctcg agcgcggcac cccgagccac
     6661 aggcctcccg cgacggtcgc ggccaccaag atactcgccg ccatcgcgag ccatcgcctg
     6721 cgatacgccg gcgcccgcag cggcggcgtc gcgatcccgg gcgcagccgt tggaccggcc
     6781 tgcggcacgc gcagggcgcg ctcgagccgc gattcgaagc gcagcagccg atccgtatac
     6841 aaattgcact cctcgcaacc actgcggtgc tcgcgcaggg tcgggtccgg gtcgtgcgga
     6901 tcggccatca gcgtccgtcg gtaagtcgcg caatccatca ctcttgcggg tccaagcggg
     6961 tgtcttcgcc gcaaattgcc cgcagctgct ttcgggcgcg aaacaaacgc gtgagcaccg
     7021 cggcgcacga cagatcgagc tcgcgcgcaa tctcggcggt ggtgaagccc atcagaacct
     7081 gcagcaccaa gggttcgcgg taatcgtcgg gcaatttgaa cagggcggcg cgcaattcag
     7141 cgaggtcttg ttcctcggcg acggccagcg tcggttcctc tcgcgccacg agttcgtcga
     7201 cgtccacggt cacgaagcgc ttgcgctcga agctgcgtgc gtattcgcgc cggatgatgg
     7261 tcaagaacca gggtttggcc gcggcttcat cctgcagcgc atcctgtgcc ttccacgcgc
     7321 gcagcatgct ttcttgcacg acatcctcgg ccaacgcacg gtcgcgggac aaccagaagg
     7381 caaagcgcaa cagatccgga cgcagggatt gacataacgc ctgaaaccgg gcgcgcttct
     7441 gatttccgcc aacctgatcc ttcatgctta aatcagcacc cggcataaga tgtctcgcct
     7501 gcgatattac cgcgttacgc cgctgccgcc gacggccggg gggccaaacc gggggcagga
     7561 gtgccgcgta cgctgcgctg aggttattgc caggagtcac atcgagatga ccggaaacac
     7621 cgctaattca tttccgagta cccgcctgcg gcgcatgcgg cgcgacgagt tttcccggcg
     7681 attgatgcgc gagtcgacgc ttggcgtgga cgcactgatt tatccggttt tcgtggtcga
     7741 aggccgcaac cagcgccagc ccgtggcctc gatgccggga gtcgagcgct tgtccatcga
     7801 ccagctgctg cgcgagtgcg aaaccctgct cgcgctcaag atcccggcga tcgccctgtt
     7861 tccggtcacg ccgcaggaga agaagaccct ggatgcgcgc gagtcctgga atccgaacgg
     7921 catcgcccaa acggcggtgc gcgctgtgaa aaaggagttt ccgggtctcg gtgtcatcac
     7981 cgatgtcgct ctcgatccgt tcacgaccca tggccaggac ggcctcatcg acgatgcggg
     8041 ctatgtgttg aacgatgtga gcgttgaaac gctggtgcgc caggcgcgct cgcatgccga
     8101 tgccggagcc gatgtggtgg cgccctccga tatgatggac ggccgcatcg ccgccatccg
     8161 cgctgcgttt gaaggcgcgg gcctcgccaa cacccggatt ctcgcctatt ccgccaaata
     8221 cgcgtcgagc ttctacggcc cttttcgcga ggcggtgggc tcggccgggc agctcggaaa
     8281 gggcggcaag cacacctacc agatggatcc gggcaactcg aacgaggccc tgcgcgaagt
     8341 ggctctcgat atcgaggaag gagccgatat ggtgatgatc aagccaggtc ttccctacct
     8401 ggatattgtg cggcgcgtca aggatcgatt cggaatgcca acctttgtct accaagtcag
     8461 cggcgagtac gccatgatca tggctgcggc gcaagccggg tgggtggatg aacgcgccgt
     8521 ggcgctggag acgcttttgt ccatgcggcg cgctggggcc gatgggattt tgacatattt
     8581 cgcgaaacgt gcagcggagt ggctcgcaga gtgaattcaa acgtggggac agacgcaccg
     8641 gatcagtacg gggtagtggg ccacccgatc gcgcatagct ggtcgccatt catccatggg
     8701 atgttcgcaa agtcgaccgc ccagaatctg gtgtaccggc tgttcgacgt gccggcggac
     8761 aatttccggc gcgaggcgct gcgtttgttc gcgagcggcg tgcgcggact gaacgtcacg
     8821 ctgccgcata agcaggcggc cgcggaactg gtcaacgaac tcacgccccg ggccgaacgg
     8881 gcgcaggccg tcaacaccat cgcctttttc gaggacactt ccctgctcgg agacaacacc
     8941 gatgggctgg gactcaccgc cgatctcgag cgcaatctca acatcaatct cgccgacaag
     9001 cgcgtcttga tactcggcgc cggcggcgcg gtgcgcggcg tgctcggacc cctcttggag
     9061 cgcgagctgg ccgaagtgat catcgccaat cgcacccccg atcgggccca taagctggcg
     9121 accgaattcg ccgacatggg ccagatctcg ggctgcgcat tcgctgaaat tcgcgggccg
     9181 agctacgaca tcgtcatcaa cgcgacctcg gcgggtttgc agggcgaaat gcccgagttg
     9241 ccggcgggct tggtcggtgc gcgcacgatc tgctacgaca tggcgtacgg ccgcggccac
     9301 actcctttca cgttgtgggc gaaatcgctg cacgccgcgc gcgtcagcaa gggctggggc
     9361 atgttggtgg aacaggcggc cgaatcgttc ctgctctggc gcggcatgcg ccccgatacc
     9421 cggccggtac tgcaagcctt gtcgcagcac gcatagcggg ctgttcagcg catggtgacc
     9481 agctcttcgg cgctggtcgg atgaatcgcc acggtgtcat cgaagtcgcg cttggtggcg
     9541 cccatgcgaa tcgccaccgc aaatccttgc agcatttcgt cggcaccggc tccgatgatg
     9601 tggcagccga cgatcctctg ttccgctccc acacatacca atttcatgtc ggtgcgcgtt
     9661 ttccgggccg tcaatgcgtg gtacatcggc gtgaaatccg cgacatacac ccggactgcg
     9721 tcgccgtatt gagcgcgcgc ttcggcctcg ctcgcgccca cggtgccaat cggcggatgc
     9781 gtgaagacca cggtcggaat catgctgtat tccaggcggc gatcgacttt gccgccgaat
     9841 aggcgatcgc tcaagcgccg cccggcggca atcgcgaccg gcgtcaactg ggcgcggccc
     9901 gtcacgtcgc cgatcgcata gacacccgcc acattggtat tttgaaaggc atcggtgatg
     9961 acgaagttgc cgtcgtcgac ggcgacgcct gcgttctgca ggtcgagcgc ttgcacattg
    10021 ggtgcgcggc cgacggccca gagcaggcaa tcgaagcccg cgaattcagt cccgccgtgc
    10081 gcgaccaggg tctgcaaacc tgaatcctcg cgaagcgacg ccggtatgac cctcgggtgg
    10141 atcgttattc cctgcgcttg catttcgcgc atcaaggatt ttccgagcat cgcatcgaag
    10201 ctggtcagaa gatgatcttt gcgaatgaat agttccacct ggctgccgag ttcatggaac
    10261 gcgcccgcca actcgcaggc aatgtagccg ctgccgacca ccgcgacgcg cgccggccgt
    10321 tgcggcagcg agaaaaaacc gtccgaggtc atgccatgct cggcgcccgg cagcatcggc
    10381 acgatcgccc tgccgccggt cgcgatcacg atgtgcggcg cagtcaagcg catgccgttg
    10441 acttcgagag cctgtttgtc gaggaagcgt gcggtgccgc gcacgtaggt cacgcccttg
    10501 gtggcaaggt tgcgttcgta gatgccgttc aagcgcagca cataggcatc gcgcttggct
    10561 ttgagcatcg gccaatcgct gccgcccgcg gtgacgctga aaccgtagtc ctgcgcgtcg
    10621 gcgagactca aggccacgtt cgctgcgttc cacatgacct tcttcggaac gcagccgacg
    10681 ttcacgcagg tcccgccgag ccggtgcgat tcgatcacca gggccttggc gccgaattcc
    10741 gcggcgcgct gtgcgcacgc caggccgccg ctgcccccgc cgatgctgat gagatcgaag
    10801 gcttggtccg acactgtgaa ctccataccg aagggcagcg aaaccgcaat gatatactgc
    10861 gcgggtccat aacttgcgga tgaggtgctt ggtgatcggt gtgggcgtga ccctggcgtt
    10921 gacggcggca ctgctcgtct atctcgtcgg catctacaat ggactcgtcg gtctcaagga
    10981 aaacgtcaag acggcatggg cgaatatcga cgtgctgctg aaacaacgcc acgacgaatt
    11041 gccgaagctc gtcgagacgt gcaagcgcta catgcagttc gagcaggaga cgctcgagaa
    11101 agtgatgcgc gcccgggcat ccgtcggtca agcgagcagc tccggcaata tcgccgcggt
    11161 gggtgctgcc gagcagcagc tgcgcgccgg cgtcggccgg ctgttcgcgg tcgccgagag
    11221 ctacccgcag ctcaagagcg atcaaacatt caaacagctg cagggacgga tcacgtcgct
    11281 ggaagagtcg atcgccgatc gccgcgaact ctacaacgat caggtcaatc tgaataacat
    11341 tcgcgtgcag gtgttcccgg atgtgatgat cgcgcacctg ggcggttttc tgccggcgca
    11401 gctgttggaa ttcagcagcg acgagaaacg cgacgtggat gtcggtgcgc tgttcggcac
    11461 ctgaaaaccc gcatcggcca tgagcgacga cgcgatcgaa tactggttca tcctgataag
    11521 cctcgcggcc ggctcgggat attcgttctg gtacgccttc agttcctggc gcaagaaccg
    11581 caccatctgc gatctgccgg tctcgcgcgt gcgctccgcg gcgcaaggct atgtccaaat
    11641 ggacggcatc ggcatgatgc cgcccggctc cgccaaccag tcgccgctga cgcgcacgcc
    11701 ctgtacttgg tggagctatg aggtcgagaa acgcgatagc gagggatggt gcacgatcga
    11761 cagcgagacc agcgccgcgc cgttccttct cgatgatggc acgggtcagt gcctcatcga
    11821 tccgcgcggc gcagaagtga tcccgcgcgc cacccacgtt tggtacggaa gcgaagcgtg
    11881 gccgcgtatg cgcctgccgg aaggccaggg aatctttggc cgcttgatcg accgtatctt
    11941 cagcggcagg taccgctaca ccgaacggcg cctgcagccg cgcgaactgg tttgcgcggt
    12001 cggcgcgtat cgcagcgtcg gcgggcgcga ggatgcggat ttggccgcgg ctgaattgct
    12061 gcgcgagtgg aagcgcgatc aagctgcgct gctggcgcgc ttcgacagcg accgcgacgg
    12121 ccggttgagc gagcgcgagt ggaatcgggc gcgcgacgcg gcgcagcgcg aagtggcgga
    12181 cagccgcgcc gccgcaccga tcccgcctac cgtaaacatg ttgctcgatc ccatggacgg
    12241 gcgcgcgttc gtgctcgcgg cgcgcgacgc ggcggcactc gcccgggggt tgcgcgggcg
    12301 agcgctgctc ggaatcaccg gcttcgtcgg cggcagcgcc gccgtcgcct ggatgttgag
    12361 tctcctctga tagaatcccg acgatgagca acccgctgtt ggaattggat tcgttgccgg
    12421 ctttcggccg catcgaggcg cgccacgcgc gcccggcgtt gctcaaggtc ctcgccgaga
    12481 atcgagcgcg tctgtcggaa ctgacggcac aggcgcagcc gacattcgct tcgctggtga
    12541 taccggtcga ggagttgagc taccggctga gccgggtctg gagtcccatc ggccacttga
    12601 acgcggtcgc caattccacc gcgatgcgcg aggcatacaa cgagtgcgtg ccgctgctca
    12661 ccgcatattc atcggaactg ggccagaacg ccgaattgca ggccggctat gcctatgtgc
    12721 tgcagcacga aggcaaggca ctcgatccgc cgcaacgcaa ggtggtggag aatgcactgc
    12781 gcgattttcg cctcgccggc gtcgatcttg gccccgagca gaagacccgc tatcgcgaag
    12841 ttgcgcaacg cctggcgcaa ctggcaacca aattctccga gaacgtgctc gacgccggcc
    12901 gcgcctacac ccgcagcgtc accgacgggg cgcagcttgc gggcttgcct gccaatgccg
    12961 tcgatcgcgc tgccgccgat gcgcgcgagg cgaaccagtc gggctggctg ttcaagctcg
    13021 accaacccac gtacatgacg ctgatggcca gcgccgagag cgcggatctg cgccgcgaca
    13081 tctatgaggc gtgggtcacg cgcgcgtccg aggtgggtcc gagcgccggc cgtttcgaca
    13141 acagcgccat cattgccgag atcctgccgc tgcgccatga actcgctcgc ttggtggggt
    13201 tcgagaattt tgccgattat gcgttggcga cgcgcatggc gaagagcagc aaacaggtgc
    13261 tcggtttctt ggacgacttg gcgcgccgtt gcctgccggc cgcgcgccag gagttcgcgg
    13321 atctcgagga atttgccggc cgcaagctca atgcctggga tatcgcgttt tacagcgagc
    13381 gcctgcagga acgccgcttc aaggtatcgc aagaggcgct gcgcccgtat ttcccgctcc
    13441 ccaaggtgct cgagggatta ttctccctgc tgcggcgcct gtacggcatc agcgtgcgcg
    13501 agcggcccgg cgtcggcgtg tggcactcga gcgtgcgcta ctacgatctt ctcgatcggg
    13561 acggcaagat cgtcgccgga ttcttcctcg atccgtattc gcgcagcgag aaacgcagcg
    13621 gcgcctggat ggacgaatgc gtcatcgcca aatcgttgcc ggacggaact gcgctgccgg
    13681 tggcgcagct ggtgtgcaac ttcaccgcgc cggtcggcaa cgcgccttca ctgctgacgc
    13741 acgacgaggt gacgacgctg tttcacgaat tcgggcacgg cctgcatcac atgctgacgc
    13801 gcgtggcgta ccccagcatc gcggggatca atggcgtggc gtgggacgcc gtcgagctgc
    13861 cgagccaatt catggagaat ttcgtatggc gcgccgaagt tttgccgctg atttctgcgc
    13921 atgtggacag cggcgaaaca ctccccgtcg agatgttgca gcgcctgttg ggcacccgga
    13981 ccttcaatgc ggcgctcgac accctgcggc agatcgaact cgcggcgttt gattttgaat
    14041 tgcatgcgaa cttcgatctt gagctcggcc cgcgggtcgc cgagacgctc gccggcgtgc
    14101 gcaagcgcgt cgcggtggtg ccggcagcgc ccttcaatcg aatgcccgca agcttcgcgc
    14161 acatattcgc cggcggctac gcggccggct actacagcta caaatgggcg gaggtgcttg
    14221 cggccgacgc attcgaggcg ttcgctgaag ccggggtgtt cgacagcgca accgccgcgc
    14281 ggtttcgcga ttccattctg gcccgcggcg gcagtttgga cgccatggac gctttcgttc
    14341 ggtttcgcgg acgcgaaccg gatgtacgcc cgctgctcaa acaaaccggc atcgcggcgt
    14401 gaaatgcccg ttcgtatgct tcggtgcgct gctgacggcc cggcttgccg cggctcccgc
    14461 ctacgtcagc gatgaattgg tgctcggcgt ctatgccgag caaaacagcc agggccagcg
    14521 actcgcgacg ctgcattccg gggcgagcct cgagaccttg agcgtcagcg gtgaattcac
    14581 ccaggttcgc ttgaacgacg gcaccaccgg gtgggtcaaa tcgacgtacc tcaccaccca
    14641 agagccggcc agcgcgcgcg tcaaacaact cgaggaggaa ctcgatcggc ggcatgccac
    14701 gacgcccgaa ctcgccgagg cggccgcacg cagcgaactc gagcagctca agcgccaact
    14761 cgccagccag caatccggac tcgaggcggc aaaggcggcg caacctcaag cggcagcgcc
    14821 ggccaacccg ccggcggcca gcggcgcgcc cggcgtcggc gcgcggttga gccatttagg
    14881 gtgggcagtc ggcgctgtgc tcgcggcgct cgcggtcggc ttttggctgg gatacgccac
    14941 cttggcgagg cgcgttcggc acaagttcgg cggcctcaaa gtctattgac tcggcggcgc
    15001 gcatttcacg tatttgacat atgcgcccag atgcggcgtc gaagtcgcaa agcacaagca
    15061 agcccgtagc gcagagcgcc ttaagagcct aaactcgccg taatgcatga ccttcgccgt
    15121 aacgattacg acgtcaccaa ttcggtgcaa gtcagttcca cagcggcggt gaccgccgcg
    15181 gtgcgcgaac tattcacctc tgcctttccc ggcgagtcat tcgatcgggt cggcgtttca
    15241 ttcgaagagt tcgacaaact cttcaccggc cggatgcccg gctaccatgg tgtcgacacc
    15301 gtctatcacg accgccagca ctcgctcgac atgaccttgg cgatggcgcg cttgctggtc
    15361 ggctacgaac gcacggtcga gccgcgcatg cgcttcggcg ccgagcgcgc ggtcatggct
    15421 ctggtcacct cgctgtttca tgatgcgggt tacatccggg aattcggcga tcgtcagcat
    15481 cgcaacggcg ccgagttcac tctttatcac gtgacccgca gcgcgcgctt tttggcgcgg
    15541 tttctgccga ccatcggcat ggaagattgg gtgccggttt caacccggat cgtgcatttc
    15601 accggctatg aaatcaaact cgatcagatt cagcttgccg atcctcgtga ccgtaagctc
    15661 ggacacttgt tgggcaccgc ggatttgatc gcgcagatgg ccgatcgttg ctacctggaa
    15721 aagtgccgtg accggctgta tccggaattc gtcctcggcg gcatcgccgc cgcgaccgga
    15781 accgacggtg ccttgcaagt tcgctacagc tcgggcttgg atttgttgcg ccagactccc
    15841 gagttcgtgc gcgagactcg ccagctacgt ctcgagggtg aattcgaaca cgcctaccgc
    15901 tatatagaac cgctgtttgg cggcaagaat ccctatctcg aggcgatcga ccgcaacttg
    15961 gttttcttgc accgggtatt gcgcaacgag cgctggctga tgttgaagcg caacccgccg
    16021 ctctttactt ccggcgacaa ccagctcggc caggtgcgcg gcttgatgct cacgcacctc
    16081 aaggctcttt gggcctaggg ccaatcgatc gcagcgacgt agcgcgcgtg cagctcgggg
    16141 agccgcgcga ggacctcgtc cttacccgat aatggcgcag cggcgatcgc ccggtgtaat
    16201 accgcggggc tcaggctgcg ccgcgcgctc tccgccacgg gagcaaaact gaaatgccag
    16261 ggctcgggtt ggacgcccga caataggccg cggaacgggc ggaaaaatcc gaaacgcgcg
    16321 gcattcgcct ccagccattc ggcgagcgcg gcgaacggtc cgcccgtcgc gaactcctgc
    16381 ggcgtcagct gaacctggta tcccggcgca gtggctctcg cgtcgacgag atcgacatcg
    16441 gtcccccagt gatggcggct cgcgccgggc agcgccgacc agagcagaat cgccgcgacg
    16501 cgctcggcgg cggaaagttg gctcacctcg atgcgccccc cctgagcgtc gtacatcggc
    16561 cgcgcgccgc tgaactttcc attccagatg gtcaattggc gcgaaaagtc ccggaagctg
    16621 ctctgcggcc gcacatcgaa gccatcggcg agtgccgcgc ggcgcaagtt ttggaacggc
    16681 accacgacct gggcatgcag ggcgcatgtc ggatcgggca cttcggtgat gtggctgcgg
    16741 gactgcccgg tcaattcgct gtcgttcacg acgccaaatc acgctcggcg gcaaaatcgg
    16801 cgacctggtc gaagatccgg gtcaaggtac cgacctcctg tgcgccggaa atcgtgtact
    16861 ggccatcgaa aacaaaggtg ggtaccccgg tgatgccaag aaccgcggca tggcgctcgg
    16921 cggcgagcac cccatcctgt ccggctctca agatcagcgc ggcacgcgat tcgcgctccg
    16981 atagcccggc ctcgactccc aggcggacca aaacctcgac ctcgccgata tcgcagcctt
    17041 cctcgaaata ggcgtgcatg acccgctcct tcacggccgc ttgtcggccg gaacgggcgg
    17101 catgggcaat caacaggtgc gaacggcggg tattgggcac ccgcgccatg aggtcgaacc
    17161 gaaaatcgat gccgctggcc tccccctgac tcttgagtac ggcatgcgct gcggcgatgc
    17221 gctcaggggg gccgattctc gctgccaggt acctggcgcg gtcgacgccc tcgatcggca
    17281 ggtcgggatt gagctcgaaa ggccgccaaa agacccgcgt ttcatattgc gggcgggccg
    17341 ccacggcgag ttcgaagcgc cgtttaccta tgtagcacca cggacaaatg gtgtcggaat
    17401 agatatcaat gcgcatattt cgggtccatc tgccgcggat tgtaccgggc cccttatagg
    17461 gtgtggcgtg aacgggtatc attctttagg tttaagcggg ggctgcggct ggtgtagtca
    17521 atggcacgaa gccttaaaac gaagtacttg ctgctggccc tggcggtcgg ggctgccctg
    17581 tcgctgctgc tcggcggctt gtcctactac gagcaccgca tcgatgcggc cgacgtcaac
    17641 cagatcacct atgccacggt tgcgcaaaag ctggaggctg acctcgagac gcgcgccagc
    17701 agcctgagcg aaatcaccgg tacctcgctc gcagccgcga taggctcagg caacaaggcc
    17761 gcggtcgcct cgattgccga gcggttgctc gatgagcgcg acatcgatcg ggtcgaggtc
    17821 tcggacgcac acggcacggt gctgttcatc ggcggggatg cggcgccgcg gcaggccggc
    17881 gactggttcg ccttccaaag caccattcat tcgaatctcg tcgcggcgcc ggtgcagcag
    17941 gttggaagtt tgcgcatctg gatgagccgc gccgagatgc gcgagactct tgccagcata
    18001 cgcaagcagc tggaaagcca acaaggcatg cagatcaagc gcatgcgcgg gttgctggcc
    18061 ggcatcaccc tgccgcttct cgtcctggga ttggccggcg cctggttcat tgccaggcaa
    18121 ctggcaggac ccatatccgc actggtgaag accgccgacc gaatcggcca aggcgactac
    18181 acccgacccc tcgccgtcgt gcgccacgac gaactcgggg atctgcaatt cgccctcgag
    18241 cgcatgcggc aaaagctcaa cgaaaccacc attaccaaga attatctgaa taccgtgctc
    18301 aacagtttga gcgatgccgt gttggtgacc tcgcccgaag gaatagtcaa gagctgcaac
    18361 gaggcgacgc agaggctgct cgggtatcag gaggacgaat tagtcggcaa acccttggtg
    18421 agcttcatcg atgaagtaca ccgcaatgcc ttcgatctga gcaattccgc gccggaggcg
    18481 cgcgagacgg tactgcggac ggcgagcggt caaaccattc cggtgtccat ggcaagttcc
    18541 gcgatcacca ccgaggatcc gcagttccaa ggcaacatct atgtggcgcg caatatcacc
    18601 gagcgcaagc gcgcggaacg gcgcatccgc tacctggcgc gctacgacac cctgaccaag
    18661 atgccgaacc gcatgcagtt tcagcatttg ctgcaacagg cgatcgcgcg cgcgcggcgg
    18721 cggcagcgct ccctcgcgct gctctatttg gatctggatc gattcaaaga ggttaacgat
    18781 accttcgggc acgccgccgg cgaccgcacc ctggagatct tgagcgagcg gctcacccga
    18841 aacttgagca aggacacggt catcgggcgc ctcgcgggcg acgagttcgc gatgttcatc
    18901 gacgatctgc cgatggatgt ggacaaccgt gccggcatcg gcaccttggc gcgcacgctg
    18961 ctcgatgaga tcagccgcac gttctatgtc aaccagcaag aggtgtattt gaccgccagc
    19021 gtcggggtgg ccatttgtcc ctacgacgcc gaaaacgtca tcgatctgat ccgcaacgcc
    19081 gacgcggcca tgtatcactc gaagcagaac ggcggcaatt cctgcgcctt ctacgttccc
    19141 gagatgaatg cggcggcggt cgaaaggctc atgttgaaga gcaagctgcg ccgcgcgctg
    19201 gagcgcgacg aattggtgat tctgtaccag tcgaaggtgg atctgcgcaa cggccgcgtg
    19261 gtgggtgccg aagcgctgct gcgctggcgc ttgccgggtc acggcgatat ctctccctcg
    19321 cagttcattc cgctcgccga agaaaccagc ttgatcctgg acatcggcga gtgggtgttg
    19381 aaccgcgtgt gttccgatta ccgggagtgg caacagaccg tgccggagcc gggccgaatt
    19441 gccatcaatc tgtcgctgcg ccaactcaag cagtcgacct tcatcgcgcg ctgccgcgcc
    19501 gtgttccgca aacacgaagt atcgccgacc tgcttcgagc tggaagtcac ggaaaccacg
    19561 ttgatgacgg acccgaaacg caccatcggg ctcctgaacg agctgtacgc catgggcttg
    19621 aacctggcca tcgacgattt cggcaccggc tactcgtcgc tatcggcact acagcagttt
    19681 cccatcggca ctctgaagat cgatcagtcc ttcgtccgcg atgtcgccgt ggactccgac
    19741 gacgcggcca tcgtgcgaac catcatcgat atgggcagga gcctcgatct cgaagtcatc
    19801 gccgagggcg tcgaagccgg cgaacagctc gaatttctgc gcaaacatgg ttgctattac
    19861 ggtcagggtc gattgttcgg cgatgccatg gaggcgagca aaatgctcgc cattctggcc
    19921 ggtcaggcgg ccggaaccgc ccaccacgcg agtttgtgcg tcccgacgct cgtgcggccg
    19981 atccgactga cctcgtagcc gcgctccgtg ctgtctgctc gggatctgac cctgcggcgc
    20041 ggtccgcagc cgctgtttgc cgaggtcaac ttcagcgtct atcgcggcga caaggtcggc
    20101 atcaccggcg ccaacggtac gggcaaatcc agtctgtttg ccgcgattca gggtgagctc
    20161 gccgccgacc gcggcgacat cgatcgcccg gccaacttga aaattgcgca tgtcgagcaa
    20221 gagattgccg ccggcgccga acgcaacgcc atcgaattcg tcctcggggg cgacgtcgag
    20281 ctttgcaacg tgctcgccgc gattgccgat gccgagcggc gcgatgcgcc gctggcgctc
    20341 gccgagagcc acgcggcctt gctggcaatc gacggctacc gtgcgcgagc ccgtgccgcc
    20401 gcaatcatgc acgggcttgg cttcaaggca tccgatcacg agcgcagcgt cgcggaattc
    20461 tcgggcggct ggcgggtgcg cctggcgatg gcacgcgcgc tcaattcgcg cgcggatctg
    20521 ctgctgctcg acgaaccgac caaccatctg gatctcgacg cgatcgtctg gctggaggac
    20581 tggttgacgg cgttcaaggg cacgctgttg atgatctctc acgaccgcga attcctcgat
    20641 gccatcatcg accgggttct gcatatcgaa aatcatgcgg tgcgcggcta ttccggtaat
    20701 tattcgcagt tcgagcagaa acgtgcggaa gaattggcgc tgcaacaggt actcgaggcg
    20761 cgccaactgc ggcgcgtcgc gcagatcacg gcgttcgtga atcgcttccg ggcgagcgcc
    20821 agcaaatcgc gccaggcgca aagccggatc aaaatgctgg aacgcatgga gcgcatcgtc
    20881 cccgcgcatg tcgacagccc gttcgagttc gaattcgccg cgccgcgggg gacgccacga
    20941 ccgctgttga cggttgaagg cgcggcttgc ggttatggcg acacgcccat cgtcgccggc
    21001 atcgacgcct cgatcgggcc gcaggaccgc attgcgctgc tcggtccgaa cggcgcgggc
    21061 aaatcgaccc tcacccggat gttggcgggc gagctgcccc cgcttgcggg tacgcgcgtc
    21121 gccgcggcgg atgtgtgcat cggctatttc gcgcagcagc agttgcagca gctcaagccg
    21181 gattgcgatg cgttttggca tctgcgcaat ttgggaggcg tggatttcgc tcagggcgac
    21241 gagcagcgcg tccgcgacca cctcggaaga ttcggattcg ccggcgagcg cgcgttcgaa
    21301 ccggtcggac gcttctcagg cggggagaag gcgcgcctga ctctggcgct ggtggtggcg
    21361 cggcggccca acttgctgct gctcgatgaa ccgaccaatc acctcgacat cgacatgcgt
    21421 cacgccctga ccgtggccct gcaaagtttc gagggcggca tgctgttggt gtcccatgat
    21481 cgtcatctga tcaagacggt ggcggattcc ttatggctgg ttgccgacgg caagctcaag
    21541 gttttcgacg gcgatttgga cgattaccag gactggctga gatcccgcgg cgcgcccaag
    21601 ccggcgcggc gcgcgcccga gagccctgcc aaggcggccg cccccgcaac gccgcgcgcg
    21661 cgcttgagcc ggctgcaccg cgaaagcgag cagatcgaac tgcggctgac ggcgatcgcg
    21721 gccgaacgcg accggctcga ggcgcagttg tgccaggatc cgatgcaccg tggcctgcag
    21781 gcgcaacacg ccgatttgac ccgcgatgcc gccgccatgg aagttcgctg gctggaagtc
    21841 ggcatggcga tcgaggcggc gcaagccggg atcgacaccg aataaactac ggggattgtg
    21901 aattcttggc gagcacggca gcggcggcgg caacgatctc cccgagcgcc gcatcggccg
    21961 cctgcgcata ggcatcgacg atggccgtca gccggttttg cgcagcgggc ttcagcgcat
    22021 ccgcctcgaa tcgaccgagg atgcggcggt cgccggaacg gccgagccgg gccagcaatc
    22081 gcacgtgcac ggcgggcgcc gccgaagcct gcgtgtattc agcctgaaag tcgctgaatt
    22141 cgagttccag ccagtagctg cttgcgaaca ccgacgtgtc gccgctgacg gtgcgcagat
    22201 tgccgtgcag ctgaaattct cgaaccgcga aatcttgcag cacctcagtg agcggtccgc
    22261 tccaacgcgc atcggcgaaa taatccaggc gccgatccgg gtacagcacg gcgatgcggt
    22321 ccatttggag tccggcgggc attttgggtt tgagaatcgc cagatcgacg gggactctcg
    22381 cgtccggcgg cgcttgcgcg ggcggcgccg ccgccagcac gtagatggtg gggggcgcga
    22441 tcttgctttg aaacagtccc gagcaggcac tgaggcacgc ggccgcgaga atcaccgccc
    22501 gccggatcgt acggctcact tcgccacctc gacgccgcgg tagttgggct catagatgag
    22561 ctgcgacgga tcctgtttca ggctgcgcga cagatcacga aaatcacgcg ccgcttgccg
    22621 gctctcgcgc agcagttgct cgaactccgg gagactttgc tgggtaaaac gcgacacccc
    22681 cggttcattg tcggcgatga attgctgcag gcgctccgag gtcttcgcca gattgtcgga
    22741 aacttgccgg atgttggcga cggcggactc gatatcgggc gtcgcgtgag tctcgatgcg
    22801 gcgcaggtcg gaggcggctc cctgtacttc ctgtgtcatg cggcgaacat ccgccaccaa
    22861 ctcctgcacc tcgcgaaacg taccgggaag atgttcgctg gcgaggcgta cattggtgag
    22921 cgtcgtattg aatgagcgca cgttgtcgtc gctgaatacc tggttgaatc gatccgccaa
    22981 ttcgaccagg tgcgtggtc
//
LOCUS       DASWZT010000554        22541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_43313, whole genome shotgun sequence.
ACCESSION   DASWZT010000554 DASWZT010000000
VERSION     DASWZT010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 22541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22541
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43313"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..2030
                     /locus_tag="VGE92_15815"
     CDS             162..2030
                     /locus_tag="VGE92_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315349.1"
                     /translation="MATKAVRRDRDDLYTLPGAIALLLLCVMLWALSHLYRGIFHDAS
                     LYTLQALARLHPGSLSQDVFLRLGSQDRYTIFSSIFAAASMGLGPENAAAWLTFASQL
                     VLLAAAFSLARAVLPSPLALLAVGVLLAIPGDYGAYRIFTCIEQFLTPRMAAEALVLA
                     AIAAALSARHLAAALFILLSLLVHPIMASAGIAVGVCLCWVIPRPRLSATVLAAAAAT
                     FVCLGAFAPAAVGGRFDAEWLQLVRERSPYLFLTNWQLDDWGRVATTLSTLAIGSAAG
                     SARALCQATLLTTLGGFALAFIAGDSLHLVLFLQWQPWRWEWLGTVAAALMLPMIIPA
                     CWSKDLPGRAAALLLASAWIFGATAFALMAPLAPGVLLLGRRLARGEARLILGGACAM
                     LAIAVAWRVATNFQFTDAHYLDLAIPLWMRRAVSFTRDGSLALALIGFTGWLAYRPRG
                     RVGCAAIAVLAGLACVAVLRPAWAQWTVRDFPPQRVAQFATWRERIPPGTDVFWPDSP
                     LAAWLLLDRPNYLSATQTSGMVFSRNAALELERRARQLQSVVPPQKFLGWDSAAPGLG
                     LSTPQLQALCSLAVFDFLVTGADLGFAPLDVVAGNSAHKPALRLYRCQARAAAAAT"
     gene            complement(2000..2914)
                     /gene="lpxC"
                     /locus_tag="VGE92_15820"
     CDS             complement(2000..2914)
                     /gene="lpxC"
                     /locus_tag="VGE92_15820"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004881498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEY1315350.1"
                     /translation="MLNQRTLKNSIRATGVGLHTGKKVLMTLRPAQPDTGIVFRRTDL
                     DQAVDIKAHAENVGDTTMGTTLHRGDVKVSTVEHLLSALAGLGIDNALVEVSAGEVPI
                     MDGSAGPFVFLLQSAGIEEQKAPKRFTRILESLQVEDGDKWARFDPYDGFKVNFEIEF
                     NHPTFKKHSQVASMDFSTTSFLREVSRARTFGFMRDLESLRARNLALGGNMDNAIVLD
                     DYRVLNEDGLRYEDEFVKHKILDAIGDLYLLGHSLIGEFSGYKSGHALNNKLLRTLID
                     TQSAWQEVTFESLADAPISYVAAAAARA"
     gene            complement(3053..4240)
                     /gene="ftsZ"
                     /locus_tag="VGE92_15825"
     CDS             complement(3053..4240)
                     /gene="ftsZ"
                     /locus_tag="VGE92_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY1315351.1"
                     /translation="MFELMDSYSQSAVIKVLGVGGGGGNAVSHMVQAGLEGVDFICIN
                     TDAQALKHSKVRTALQIGCNITKGLGAGADPEVGRQAAMEDRDRIIELIEGCDMLFIT
                     AGMGGGTGTGAAPVVAQVAKELGILTVAVVTKPFEMEGNKRSRVADQGMLELSKYVDS
                     LITIPNQKLLSVLGSSTTLLDAFKAANTVLQGAVQGIAELITRPGLINVDFADVRTVM
                     SETGMAMMGSGSAAGDDRAREAAEAAISSPLLEDINLSGAQGILVNVTAGMDLSIGEF
                     QEVGNVIKQFASDEATVVVGTVIDPDMANQIRVTVVATGLGKPVASARPAPIQQAAAA
                     AIAPALTMRVVRPKTPMAPTDYAMLDKPTVQRKRAVGDDMRSEIDSEDLLDIPAFLRR
                     QAD"
     gene            complement(4277..5515)
                     /gene="ftsA"
                     /locus_tag="VGE92_15830"
     CDS             complement(4277..5515)
                     /gene="ftsA"
                     /locus_tag="VGE92_15830"
                     /inference="COORDINATES: protein motif:HMM:NF007009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY1315352.1"
                     /translation="MAKRSDRNLLVGLDIGTSKVVAIVGEIKTDGALEIIGIGSHPSR
                     GLKKGVVVNIESTVQSIQRAVEEAELMAGCEIHSVYAGIAGSHVRSLNSHGIVAIKDK
                     EVVQGDVERVIDAAKAVAIPADQKILHVLPQEYIIDSQEGIRDPIGMSGVRLEAKVHI
                     VTGADSAAQNIVKCVQRCGLAVDDIVLEQLASSYAVLTEDEKDLGVCVVDIGGGTTDI
                     AVFGGGAIRHTAVIPIAGDQVTNDIAVSMRTPTQYAEDIKIKYACALSQLANPDETIE
                     VPSVGDRPPRRLARQTLAEIVEPRYEELFGLIRDELRRAGLEEQVATGIVLTGGSSKM
                     EGAIELAEEVFHMPVRLGVPQYVSGLVDVVSNPIHATGVGLLLYAKGNLDAQRAEAPL
                     LAGGMKNIFERMKAWFQGNF"
     gene            complement(5533..6351)
                     /locus_tag="VGE92_15835"
     CDS             complement(5533..6351)
                     /locus_tag="VGE92_15835"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEY1315353.1"
                     /translation="MNANWLMRRNRFKKTPRGEKAPRKWPEFVSAWRTYARRAGLLLL
                     LSGGLAGLTWGLDRPLRVISMDGSFQRVTPGQIEKAVAPYSQAGFMSADLDGIQRAVE
                     ALPWVEHARIQRRWPNSLHVTVIEQTAAARWGESGLLNTRGELFVKSATHVPAELPHL
                     SGPDGTESQVALRYLALQGRMLEAGMRIAALRLDERGAWEVDLDSGVTVRLGRRDVDE
                     RIDRFMHTASQVIAHRLTEIAYIDMRYSNGFAIGWRNPALPAAAPAKHADDADV"
     gene            complement(6348..7316)
                     /locus_tag="VGE92_15840"
     CDS             complement(6348..7316)
                     /locus_tag="VGE92_15840"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEY1315354.1"
                     /translation="MTKLQIKTQFSRITRAADFGKTAVLLEGDSSEREISLISGNAVL
                     AALQRRGVDAHAFDPQERPLAELESGGFARAFIILHGPGGEDGTMQGALEWLGVPYTG
                     SGVLASALTMDKLKTKRVVVGAGYAAPEYAVLSSPADLPGALERIGLPMMVKPASQGS
                     SVGMTKVKIAADLPRAYAEARAVDPIVFGERFISGDEYTVGVLHDAALPSICIQPATE
                     FYDYQAKYFRNDTQYHCPSGLDAAAEAVLRAAALAAFRVTDCCGWGRVDFMREAASGK
                     FYFIEINTTPGMTDHSLVPMAARQAGIDFEELVWRVLESSFIRERK"
     gene            complement(7313..8218)
                     /gene="murB"
                     /locus_tag="VGE92_15845"
     CDS             complement(7313..8218)
                     /gene="murB"
                     /locus_tag="VGE92_15845"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEY1315355.1"
                     /translation="MNAQRSRGLAAEFAARVLYGVSMAKHTSWHVGGPADVFFSPRDV
                     ADLADFLRQLPPQMPVLMIGLGSNLLVRDGGIRGAVIATHGALGALELLSATRIHAEA
                     GVPCARIARQCVKWGLGPAEFFAGIPGTLGGALAMNAGAWGGETWRHVVEVDALDRRG
                     VRHTRAAAEYDIGYRRVRGPQDEWFVGARLEFAHTPGVNADAIRELLDKRKQSQPIGE
                     WSCGSVFTNPPGDHAARLIESAGLKGYRLGDASVSEKHANFIINHGAACAGDIEALIA
                     HVQRSVARVHGVELNTEVRIVGVPA"
     gene            complement(8215..9660)
                     /gene="murC"
                     /locus_tag="VGE92_15850"
     CDS             complement(8215..9660)
                     /gene="murC"
                     /locus_tag="VGE92_15850"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003654096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEY1315356.1"
                     /translation="MSERPNPVERRRMDDRMRRIHRIHLVGIGGSGMGGIAEVLLNLG
                     YAVQGSDLKENSVTQRLARLGAHIFIGHSALHLGESDVVVVSTAVSRSNPEVVAAQAK
                     RIPVVQRAEMLGELMRFRYSIAVAGTHGKTTTTSLVASVLAEAGFDPTFVIGGRLKST
                     DSNAKLGAGRYLVAEADESDASFMHLQPMIAIVTNIDNDHLTTHDGDFSRLKQSFVEF
                     LHNLPFYGLAVLCGDDEQVCSILQNVARPFVTYGFAPEADIRAVEVVRDGLQSRYQAL
                     RAGHGPLPITINLPGRHNVLNSLAAVAVATELDIPADAIQRALANFQGIDRRLQQLGE
                     IQWQGGKALLVDDYAHHPTEVAATLDAVRQAWPSRRLVLAFQPHRYTRTRDLMDDFGR
                     VLGGCDVLLVTEVYGAGEAPIPGADGRAICRAVRTRGLLEPVFVERVDELAESLRGIL
                     RDGDVLLTMGAGNIGAVSHELKNRFATGAAA"
     gene            complement(9657..10763)
                     /gene="murG"
                     /locus_tag="VGE92_15855"
     CDS             complement(9657..10763)
                     /gene="murG"
                     /locus_tag="VGE92_15855"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEY1315357.1"
                     /translation="MSAPATQVARGPVLIMAGGTGGHVFPALAVAKVLRERGIGVVWL
                     GVPGSMESRLVPANGFPIEWVRVGGVRGKGLKTWLLAPWRVAKAVVQAIGVLRRVRPQ
                     SVLGAGGYVSGPGGVAAWLLRIPLLIHEQNAIAGMTNRMLSRFASNVLEAFPGSFSPR
                     VHAVAIGNPVRADIAAVAPPELRFAQRNTRSRLLVFGGSQGAVRLNAMVPEALARVAP
                     EIRPQVRHQTGERGLEAARAAYAEAEVEAEVMPFIDDMASAYAWADLAVCRAGAMTVA
                     ELQAAGLGAIFVPLPVATDDHQTKNAEVMVKSGAAQLLQERDLTPERLGSAISRLVAD
                     RNQLLKMAQAARASRVIDAATRLADLCVAAGAHA"
     gene            complement(10760..11947)
                     /gene="ftsW"
                     /locus_tag="VGE92_15860"
     CDS             complement(10760..11947)
                     /gene="ftsW"
                     /locus_tag="VGE92_15860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEY1315358.1"
                     /translation="MTAATMSYGVRARERMPFAWDYLSLGLAASLLLVGLIMVTSASM
                     SIAARDLGNPFYFLERQFIFGLAGIAFAWIITRVPTEVWDKYSLVLLCCGLLLLFLVL
                     IPGVGATVNGARRWLRVGPVNFQVSELAKVLVLTWVCSYCVRKRTELQIALSGLAKPV
                     GLLAVAALLLLCEPDFGATTVLFATGIAVLFVAGARLRYMLLLVAAATLAFSVLALTS
                     TYRLKRLTGFLHPWDDPFNGGFQLTQSLIAIGRGAWFGVGLGSSVQKLFYLPEAHTDF
                     VFAVLAEELGLVGVLGVIALFMALVWRAFKISQMAAQAGLRFQSYLALAFGVWLGLQA
                     MVNIGVNMGVLPTKGLTLPLLSYGRSSLLVSLAWLGAVLRIFHEVKCSSRSAVMRLPG
                     GVR"
     gene            complement(11944..13272)
                     /gene="murD"
                     /locus_tag="VGE92_15865"
     CDS             complement(11944..13272)
                     /gene="murD"
                     /locus_tag="VGE92_15865"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEY1315359.1"
                     /translation="MHSVVVGLGKTGASCLRYLSRHGIAASATDSRPVPPGLAELGEL
                     AKSADLRLGGFDLSLLDGASQVLISPGVSLKEPIARAARDRGIEVVGDVELFARNVTA
                     PVIGVTGTNGKSTVTSLVARMAAAAGRNVLAGGNLGVAALDLLDQPTPDLYVLELSSF
                     QLETTTSLELTAAVVLNVTPDHMDRYPSIAAYAAAKSRIFSNAQTMVLNIDDPLVAAM
                     RGAGPKRSVTFSIESEHADFTLRRIDSQTFLTRRGVALMDIDRMKISGLHNAANALAA
                     LALGDAVGLPVPSMLEALESFPGLAHRSEWIADVDGVRYIDDSKGTNVGATIAAVAGM
                     QGPLVMIAGGEGKLQDFTPLAAAFRGKVRHAVLIGRDAQAIAAALSGVCPTDTVGSMQ
                     DAVALAARVAKAGDAVLLSPACASFDMFRDYADRGDAFAVAVHTLKERPT"
     gene            complement(13272..14357)
                     /gene="mraY"
                     /locus_tag="VGE92_15870"
     CDS             complement(13272..14357)
                     /gene="mraY"
                     /locus_tag="VGE92_15870"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008045924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEY1315360.1"
                     /translation="MLLALTEYLARFYTGFHVFQYLTLRGILAAVTALALALFIGPTM
                     IAMLSRYQIGQQVRSDGPQSHLQKSGTPTMGGGLILIAMVLGTLLWADLSSRFVWILL
                     ATTLGFGLIGFYDDYLKLVVGNSKGLAARYKYLAQSLVGIATIMTLHHLRLSPAETSL
                     YVPFFKTVAVPMSTAAYVALCYFVIVGTSNAVNLTDGLDGLAIMPAVMVAAALGVFAY
                     VTGNFKFATYLQIPYIPGVGEALIFSATLVGAGLGFLWFNSYPAQVFMGDVGALAIGA
                     SLGTLAVIVRQEIVLFVMGGVFVLETVSVMLQVASFRLTGKRIFRMAPIHHHFELKGW
                     AEPKVIVRFWIISFVLVLTGLATLKLR"
     gene            complement(14362..15717)
                     /gene="murF"
                     /locus_tag="VGE92_15875"
     CDS             complement(14362..15717)
                     /gene="murF"
                     /locus_tag="VGE92_15875"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEY1315361.1"
                     /translation="MKRTLHEAATAVGGQLIGEDRPFGAVSTDSRTLAPGTLFAALRG
                     PKFDGRDFVTAAATRGAIGAIVERAVSHAVPQIVVADALRALQQLGQAWRAGFDVPVV
                     AVGGSNGKTTTKEMTAAILSRMGVVMATHGNLNNHIGVPLTLMRLEASHRSAVIEMGA
                     NRIGDVAELVRLARPTVGLITNAGAEHLEGFRTLDGVAQGEGEMVSGLEPAATAVINA
                     DDAYAGYWRSVATARRIVTFGVHSAADFTAQDSKQTIERGEFLTRFTLRCPLGQHPIT
                     LKAGGAHNIGNALAAAAAAGAAGASLEEIALGLADFRAVAGRLQLKAGARDSWIIDDS
                     YNANPSSVRAGLEVLRALPGATWLVLGDMAELGEFAEDSHAHMGAYARDCGIKRLFAV
                     GPQSSRAVETFGSGAEWFADADSLTRRLQAEIAPGVTVLVKGSRVNRLERVVQALTGA
                     N"
     gene            complement(15714..17207)
                     /locus_tag="VGE92_15880"
     CDS             complement(15714..17207)
                     /locus_tag="VGE92_15880"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEY1315362.1"
                     /translation="MSVSLMWLLEGIAKLPDAARCEVHDLTLDSREVRAGSLFLALRG
                     RSVHGVKFAAEAAARGASVVLWEPAAGIELPALPARVFAAAIPNLRALVGRIADRFFK
                     SPSAQLRITGITGTNGKTTCAFLLAQCLERLGSRAAYIGTLGWGRIASLAPASLTTPD
                     AVTLHRELAELHAAGVRDVAMEVSSQALDQDRVAAVRFHAAAFTNLSRDHLDYHQTMT
                     AYGAAKARLFMAPDLEHIVVNVADAFGRELAQNYAGRAPLTAVWIGAGDTLPTRRTLH
                     ATQVTLDVHGVSMQLDGSFGEARVDTRLLGRFNADNSLIVIACLLSLGASLAEAARVL
                     AECNPPPGRMEVVDPDARGKPLAVVDYAHTPDALAKALGALREHCAGSLWCVFGCGGD
                     RDPGKRPLMGSIADELADRVIVTDDNPRSEDPQAITGAIISGIKSRTVRVIHDRGEAI
                     GTALKEARATDVVLIAGKGHEDYQIYGDTRRSFSDRLEARRHLGVAA"
     gene            complement(17204..18934)
                     /locus_tag="VGE92_15885"
     CDS             complement(17204..18934)
                     /locus_tag="VGE92_15885"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY1315363.1"
                     /translation="MKLRSGDNDTQARSYRWRSSAILGLLMVGAVGLVARAVELQLLD
                     HGFLAKQGDDRSMRVVKIAAHRGAITDRNGEPLAVSTPVDSVWVNPQELNDNIDELPK
                     LAKALKQDQQSLARRITSNLDREFLYLERHMPPEQAAHIKALGIPGVYLLREYRRYYP
                     AGEISGHVVGFTTIDDKGQEGLELGFDQLLNGEDGAKRVIQDMYGRYVENVESIRAPQ
                     PGRDLLTSIDLRIQYLAYRELKAAMQEYRARAGSVIVIDVDTGEVLAMVNQPAYNPND
                     REQLKAGLYRNRAATDIFEPGSSIKPFIMAAALASGQYRADSVVDTSPGFLKVGTKVF
                     EDEQNLGAIDLATILAKSSNVGTAKVALSLQPEQIWSTLTGLGLGQVTGSGYPGESAG
                     MLSHYSHWRPIGIATLSHGYGLSVTPLQLAHAYATIGAGGIKRPISFERVQGPVPGER
                     VLDSKVCATLVQLMEQVVEKGGTATRASLIGYRVSGKTGTAWKSIAGGYSTDRYMAVF
                     AGLVPASHPKLATVVVIDEPSGDLHQGGTVAAPVFASVMSGALRLMDVPPDDLQNVPA
                     ATLVQANETP"
     gene            complement(18931..19209)
                     /gene="ftsL"
                     /locus_tag="VGE92_15890"
     CDS             complement(18931..19209)
                     /gene="ftsL"
                     /locus_tag="VGE92_15890"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEY1315364.1"
                     /translation="MKHRSWLSLAVPVLWLAVLGTAVQVIYARHKARDMFVHLEKLNG
                     DRDSLDMEWGRLQLEQSYWSSHAFVERVANAKLQMNLPQTRDVRIVRP"
     gene            complement(19206..20174)
                     /gene="rsmH"
                     /locus_tag="VGE92_15895"
     CDS             complement(19206..20174)
                     /gene="rsmH"
                     /locus_tag="VGE92_15895"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015271715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEY1315365.1"
                     /translation="MQDIAGVHTPVLLPQVLAALNIRGSGTYLDATFGRGGHAGAILH
                     NLFANGRLLCLDRDSAAIADAKSRFAADRRVTLFLARFSALAQCADQVEAGLKFDGIL
                     FDLGVSSPQLDDAARGFSFMQDGPLDMRMTAQGGFSAADVVNGASLHELIRIFRDYGE
                     ERQAARIARAIVKDRESQPFERTLQLAEMIARVAPRYDATGRPVHKHPATRVFQALRM
                     HVNGELDELEAGLNAALERLAPAGRLAVISFHSLEDRLVKQFMRRHAMADPMYAGLPN
                     IPPQARPTLKLVGKSIEPDANEAFRNPRARSARLRVAERLPQRAAA"
     gene            complement(20187..20663)
                     /gene="mraZ"
                     /locus_tag="VGE92_15900"
     CDS             complement(20187..20663)
                     /gene="mraZ"
                     /locus_tag="VGE92_15900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEY1315366.1"
                     /translation="MGRQTHPIMFRGANKLTLDVKGRVVMPTRYRERLQERCGGKLVV
                     TVDKDQCLLIYPLPDWEEIERKLMRLPSMNPQSRRLQRLMVGHATDLELDGHGRVLLP
                     PNLREFSLLTRDAMLIGQGLYFELWDEARWNERRDEWLAGDGSTTDLPTELETLSL"
     gene            complement(20968..>22541)
                     /locus_tag="VGE92_15905"
     CDS             complement(20968..>22541)
                     /locus_tag="VGE92_15905"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing
                     glycosyltransferase family protein"
                     /protein_id="HEY1315367.1"
                     /translation="DQALALNPQLFQVWFHRGNVLRQMRRLTEALSSYEQAVKLQPDH
                     AAGHYNCAVLLERCGRYRDALSSYERAVSIYPEFHEAHFNRAGVLKQLGEREAALASY
                     DRAIAAKPDYAEAHSNRGVLLQELGRRDEALASCDRAISIRCNYPEAHFNRGTLLQAN
                     MQFEAALASYDRAIALRPDYAEAYCDRAGVLLEVGRVNEALVDYERATTIRPDFAEAN
                     LNRSLALLLHGDYEEGWRLHEWRWKNAERLQTEWRSFKQPLWLGEEEIFGKTLLLYCE
                     QGLGDTLQFCRFAKTVAERGATVILEVQAPLVSLLKDLDGVSLVVPEGGLLPPFDYHC
                     PLLSLPLALKTTLDTIPAPRRYLHSQASKIPEWESRLGPRNGPRIGLVWSGNPKNGRD
                     HNRSVRLLDWIEHLPRDFQYICLQREIRPADREVLEANRWISTFDPDFTETAALCECV
                     DLVISVCTSIAHLSAGLGRNTWVMLPFAADWRWLVGRIDSPWYPSVTLYRQHALGDWQ
                     GLFARVAADLHQVFR"
BASE COUNT         4095 a         7449 c         6950 g         4047 t
ORIGIN      
        1 tcccgtatcc gctatttcac cggtcccatc cgcaaatcgg tcgcagccgc attggcgctc
       61 gaggtctctg ccgttcgaat tcgcgtggtg cagccgatcg cctagaggcg cgtgcgatgc
      121 cgatcacctc gccggggcac cggcacgaca taatgggccg catggccacg aaagctgtga
      181 gacgagatcg cgacgacctg tataccctgc caggcgccat cgcgctcttg ttgctctgcg
      241 tcatgctgtg ggcgctgtcg catctctacc ggggcatttt tcacgatgcg agcctgtaca
      301 ccttgcaggc gctcgcgcgc ctgcaccccg gttctttgtc gcaagatgtc ttcttgcgcc
      361 tcggctcgca ggatcgctac acgatcttca gttccatatt cgcggccgca agcatggggc
      421 tcggcccgga gaatgcagca gcttggctca ctttcgcgtc ccaattggtc ttgctggcgg
      481 cggccttcag cttggcgcgc gcggtgctgc catcgccgct cgcgctcctc gccgtgggcg
      541 tactgctggc gatccccggc gactatggtg cctatcgtat ctttacttgc atcgagcaat
      601 ttttgacgcc gcgaatggcc gcagaagcgc tcgtccttgc cgccatcgcc gcggcattga
      661 gcgcgcgcca tctcgcggca gcgctattca tcctgctctc gctcctcgtg catccgatca
      721 tggcatccgc cggcatcgcc gtcggcgtgt gtctttgctg ggtcatcccg cgcccgcgcc
      781 tgagtgcgac ggtcttggca gcggccgccg caacgtttgt ctgcctcggg gcgttcgcac
      841 cggccgctgt gggaggccgt ttcgatgccg agtggctgca actcgtgaga gaacgaagtc
      901 cgtacctgtt cctgacgaat tggcaactcg acgattgggg acgggtggcg acgacgctct
      961 cgacattggc gatcggcagc gcggcgggca gcgcccgcgc cctgtgccag gcgacgctgt
     1021 tgacgacgct cggcggcttc gctttggcct tcatcgccgg cgattccctg cacctcgtac
     1081 tgttcttgca gtggcaaccc tggcgttggg aatggctggg cacggtcgct gcggctctga
     1141 tgttgccgat gataatcccg gcgtgctggt cgaaggacct ccccgggcgt gccgctgcat
     1201 tgctgcttgc ctccgcctgg attttcggag caaccgcctt tgcgttgatg gcgccgctcg
     1261 cgccgggcgt gctgctgttg ggaaggcgtc ttgcgcgcgg cgaggcgcgg ctgatattgg
     1321 gcggagcctg cgccatgttg gccatcgccg ttgcctggcg ggtcgccaca aattttcaat
     1381 tcacggacgc gcactacctt gacctcgcca tcccgttatg gatgcggcgc gctgtgagct
     1441 tcacccggga tggcagcctc gcgctcgcac tgatcggatt caccggatgg ctcgcgtacc
     1501 ggccgcgggg ccgggttggc tgtgccgcca tcgccgtact cgccgggctg gcatgcgtcg
     1561 ctgtgctgcg cccggcctgg gcccaatgga cagttcgcga cttcccgccg caacgggtcg
     1621 cgcaattcgc gacctggcgc gagcgcattc cgccgggaac ggatgtattt tggcccgact
     1681 caccgctcgc ggcctggttg ctcttggata gaccgaatta tctgtcggcg acgcaaacat
     1741 ccggaatggt gttttctcga aacgcggcgc tcgaactcga gcggcgagcg cggcagttgc
     1801 aatcggtcgt gccgccgcag aaattcctgg gttgggatag tgccgcgccc ggtctcgggc
     1861 tttcgacgcc gcagctgcaa gcgctctgca gtctggcggt gtttgatttt ctggttaccg
     1921 gcgccgatct cggattcgcg ccactcgatg tcgtcgcggg aaattcagcg cataagccgg
     1981 cgctgcgcct gtatcgctgt caggcgcgcg ctgcggccgc ggcgacgtag gaaatcggcg
     2041 catcggccaa gctttcgaat gtcacttcct gccacgcgct ttgcgtgtcg atcagcgtgc
     2101 gcagcaattt attgttgagc gcatgcccgg atttatagcc gctgaattcg ccgatgaggc
     2161 tatgtcccag aagatacaga tcgccgatgg catcgagaat cttgtgcttc acgaactcgt
     2221 cttcgtagcg caagccgtct tcgttcaata cgcggtagtc gtcgaggacg atggcgttat
     2281 ccatgttgcc gccgagcgcg aggttacgcg cccgcaggct ctccaagtcg cgcatgaagc
     2341 cgaacgtgcg cgcgcggctc acctcgcgca agaacgaggt cgtcgagaaa tccatcgacg
     2401 cgacctgtga atgtttcttg aacgtcggat gattgaattc gatttcgaag ttcactttga
     2461 aaccgtcata cggatcgaag cgcgcccact tatcgccgtc ctcgacttgc aggctctcga
     2521 gaatacgggt gaatcgcttg ggagctttct gctcctcgat tccggctgac tgcaacagaa
     2581 acacgaaggg acccgcgcta ccgtccatga tcgggacttc acccgcgctg acttcgacca
     2641 gggcattgtc gattccaagt cccgccagcg ccgacagcag atgctcgacg gtggacactt
     2701 tgacatcgcc gcggtgcaac gtggtgccca tggtggtgtc gccgacattc tcggcatgcg
     2761 ccttgatatc gaccgcttga tccaagtccg tgcggcgaaa cacgatgccg gtgtccggct
     2821 gcgccggccg cagggtcatc aacaccttct tgccggtgtg cagccccacc cctgtggcgc
     2881 ggatggaatt cttcagcgtt ctctgattga gcatctcaaa tgaaaccggg gactatgtta
     2941 atacccgcaa aggtaccata ccgcgcaccc cggtctaaag ctcaactgcc caaacgggcg
     3001 cccggggagt gtaatctccg cgggcgccaa agcctggaat gtcggtctgt cgctagtcgg
     3061 cctgccggcg caagaacgcc gggatgtcca gcaaatcctc cgaatcgatt tcgctacgca
     3121 tgtcgtcgcc cacggcgcgc ttgcgctgca ccgtcggctt atccagcatg gcgtagtcgg
     3181 tcggtgccat cggcgttttc ggacgcacca cccgcatggt cagcgccggt gcgatggcgg
     3241 cggccgctgc ctgctggatc ggcgccggcc gcgcgctagc caccggcttg cccaagccag
     3301 tcgccaccac cgtcacgcgg atctggttgg ccatgtccgg atcgatcacg gtcccgacca
     3361 ccacagtggc ctcgtcggag gcaaattgct tgatcacatt gcctacttcc tggaattcgc
     3421 cgatggacag atccatgccc gcggtgacat tgaccaggat gccttgtgcg cccgagagat
     3481 tgatgtcctc gagcagcgga cttgagatcg ccgcctcggc cgcttcacgc gcccgatcgt
     3541 cgcccgccgc gctgcccgaa cccatcatgg ccatgccggt ctccgacatc acggtgcgca
     3601 cgtcggcgaa gtcgacattg atcaaacccg gccgcgtgat gagttcggcg ataccctgca
     3661 ccgctccctg cagcacggta ttcgccgcct tgaaggcgtc gagcagcgtg gtactgctgc
     3721 cgagaaccga caacagcttt tggttcggaa tggtgatcag ggagtcgacg tacttcgata
     3781 actcgagcat cccctggtcg gccacgcgcg agcgcttgtt gccttccatt tcgaacggct
     3841 tggtgaccac ggcgaccgtc aatatgccga gttccttggc gacctgcgcc accaccggcg
     3901 ccgcgccggt gcccgttccg ccgcccatgc cggcggtgat gaacagcatg tcgcaaccct
     3961 cgatgagctc gatgatgcgg tcacgatctt ccatcgccgc ctgccggccc acttccgggt
     4021 ccgcgcccgc gccgaggccc ttggtgatgt tgcagccgat ctgcaaagcg gtgcgcacct
     4081 tggagtgctt cagcgcctgc gcatcggtgt tgatgcaaat gaaatccacg ccctcgaggc
     4141 ccgcttggac catgtgcgac acggcgttgc cgccgccgcc gcccacgccc agcaccttga
     4201 tgacggcact ttgactataa gagtccatca gttcgaacat tccagtactc cttcgtttat
     4261 caatcgcaaa tcattgtcaa aaattgccct gaaaccacgc cttcatgcgc tcgaaaatat
     4321 tcttcatgcc gcccgcgagc agcggcgctt cggcgcgttg cgcgtcgaga ttgcccttgg
     4381 catagagcag caacccgacg ccggtggcgt gaatcggatt gctgacgacg tccaccaggc
     4441 cgctcacgta ctgcggtacg ccgagacgca ccggcatatg aaagacttcc tcggcaagtt
     4501 cgatggcgcc ttccatcttt gaactgccgc cggtcagcac gatgccggtc gccacctgct
     4561 cctccaatcc ggcgcgccgc aactcgtcgc ggatcaggcc gaacaattcc tcgtagcgcg
     4621 gctcgacgat ttccgcgagg gtctgccgcg cgagccgccg cggcggccga tcgccgacgc
     4681 tcgggacttc gatcgtctcg tcgggattcg cgagctgcga cagcgcgcag gcgtacttga
     4741 tcttgatgtc ttccgcgtac tgcgtcggcg tgcgcatcga cacggcgatg tcgttggtca
     4801 cttgatcgcc ggcaatcggg atcaccgcgg tgtggcgaat tgcgccgccg ccgaagaccg
     4861 cgatgtcggt ggtgccgccg ccgatgtcca ccacgcacac gccgagatcc ttttcatcct
     4921 cggtgagcac cgcgtaactc gacgccagct gctccaacac gatgtcgtcc accgcgaggc
     4981 cgcagcgctg cacgcacttg acgatgttct gcgccgcgct gtctgcgccg gtgacgatgt
     5041 gcaccttggc ttcgaggcgc acgccggaca tgccgatcgg atcgcggatt ccttcctggc
     5101 tgtcgatgat gtattcctgc ggcagcacgt gcaggatctt ctggtccgcg gggatggcca
     5161 ccgccttcgc cgcatcgatc acgcgctcca catcgccctg caccacctct ttgtccttga
     5221 tggcgacgat gccgtgcgaa ttcaggctgc gcacatggct gccggcgatg ccggcgtaca
     5281 ccgaatgaat ctcgcagccc gccatcagct ccgcttcttc cacggcgcgt tgaatggact
     5341 gcacggtgga ttcgatgttg accacgacgc ccttcttcaa gccacgcgac ggatggctgc
     5401 cgatgccgat gatttccagc gcgccgtcgg tcttgatctc gccgacgatg gcgacgactt
     5461 tgctggtgcc gatatccagc cccaccaaca gatttcgatc ggaacgctta gccatttgac
     5521 tcgttacaac ctctacacgt cggcatcgtc ggcgtgcttg gcgggcgctg ctgcgggcag
     5581 cgccggattg cgccagccga tggcgaagcc gttggaatag cgcatgtcga tataggcgat
     5641 ctcggtcaag cgatgggcga tgacctgcga agcggtatgc atgaatcgat cgatacgttc
     5701 gtcgacgtcg cgccttccca ggcgcaccgt gacgccgctg tcgaggtcca cctcccaggc
     5761 gccgcgctca tcgagacgca gcgcggcgat acgcatgccg gcttcgagca tccgcccttg
     5821 cagcgccaaa taacgcagcg cgacttgcga ttccgtgccg tccgggccgc tcaaatgcgg
     5881 caattccgcg gggacgtgcg tcgcggattt gacgaacagc tcgccgcgcg tattcaagag
     5941 ccccgattcg ccccagcgag cggcggcggt ttgctcgatc acggtgacgt gcaagctgtt
     6001 cggccagcgg cgctgaatgc gtgcgtgctc cacccagggc aatgcttcga ccgctcgctg
     6061 gatgccgtcg agatcggcag acatgaaacc cgcctggctg taaggcgcga cggccttctc
     6121 gatttgaccc ggagtgacgc gctgaaagct gccgtccatc gagatgaccc gcagcggacg
     6181 atcgaggccc caggtgagac cggcgagccc gccgctcaac agcagcagca agcccgcgcg
     6241 gcgcgcgtag gtgcgccagg cgctcacgaa ctcgggccac ttgcgcggcg ctttctcgcc
     6301 cctcggtgtt ttcttgaatc gattacgccg catcagccag ttcgcgttca tttgcgctcc
     6361 cgtataaaac tgctttccag tacccgccac accagctctt cgaagtcgat gccggcttgg
     6421 cgcgccgcca tgggcacgag gctgtgatcg gtcatgcccg gagtggtatt gatctcgatg
     6481 aaatagaact tgccggaggc cgcctcgcgc atgaagtcga cgcggcccca gccgcaacag
     6541 tccgtcacac ggaaggccgc gagcgccgcc gcccgcagca cagcctcggc tgctgcgtca
     6601 agaccgctcg gacagtggta ttgcgtgtcg ttgcgaaagt atttcgcttg gtagtcgtag
     6661 aactcggtcg ccggctggat gcaaatcgac ggcagcgccg catcgtgcag cacgccgacg
     6721 gtgtattcat cgccgctgat gaagcgttcg ccgaagacga ttggatcgac ggcgcgcgcc
     6781 tcggcgtagg cgcgcggcag gtctgccgcg atcttgactt tggtcattcc gacgctggaa
     6841 ccctgggacg cgggcttcac catcatgggc aggccgatgc gctcgagcgc gcccggcagg
     6901 tcggcagggc tcgacaacac ggcgtactcg ggcgccgcat agcccgcgcc gaccacgacg
     6961 cgcttggttt tcagcttgtc catggtcagc gccgaggcaa gtacgccgct gcctgtataa
     7021 ggcacgccca accactcgag cgcgccctgc atcgtgccat cctcgccgcc cggcccgtgc
     7081 aggatgatga atgcacgggc gaagcccccg ctctcgagct cggccagcgg ccgctcctgc
     7141 gggtcgaagg cgtgggcgtc gacgccgcgg cgttgcaacg cggcgaggac ggcattgccg
     7201 gagatcagcg atatctcgcg ttcactcgaa tcaccttcca ggagtactgc ggtcttgcca
     7261 aaatctgcgg cgcgcgtgat ccgcgagaat tgcgtcttga tctgcaactt ggtcatgccg
     7321 gcaccccgac gatgcgcact tcggtgttca gttccacgcc gtgtacacgc gcaacgctgc
     7381 gctgcacgtg cgcgatcaga gcctcgatat cgccggcgca agcggcgccg tgattgatga
     7441 tgaaattggc gtgtttctcg gataccgagg catcgccgag ccgatagcct ttgaggccgg
     7501 cgctttcgat caagcgcgcg gcgtgatcgc ccggaggatt ggtgaacacg gatccgcagc
     7561 tccactcgcc gatgggctgc gactgttttc gcttgtcgag caattcgcga atcgcgtcgg
     7621 cgttgacccc gggagtgtgg gcgaattcga gccgcgcacc gacgaaccac tcgtcttgcg
     7681 gtccccgcac gcgccgataa ccgatgtcgt actcggcggc cgcgcgggtg tgccgaacgc
     7741 cgcggcggtc gagcgcatcg acttcgacca cgtgacgcca ggtctcgccg ccccaggctc
     7801 cggcgttcat cgccagcgcg ccgcccaaag tgccgggaat tccggcaaag aattccgccg
     7861 gccccaatcc ccatttgacg cattgccttg cgattcgcgc acacggtacg ccggcctcgg
     7921 catggatgcg cgttgcgctc aacagctcga gcgcgccgag cgcgccgtgc gtcgcaatca
     7981 cggcgccgcg gattccgccg tcgcgcacca gcagattgct gccgagccct atcatgagca
     8041 ccggcatctg cggcggcagc tggcgcaaga aatccgcaag gtcggccacg tcgcgcggcg
     8101 agaagaacac atcggcaggc ccaccgacat gccaggaggt gtgtttggcc atcgacacgc
     8161 cgtacagaac ccgcgcagcg aattcggcag ccaatccgcg agagcgctgc gcattcatgc
     8221 cgcagctccc gtcgcaaagc gatttttgag ctcgtgggag acggctccga tgttgccggc
     8281 gcccatcgtc agcagcacgt cgccgtcgcg caatatgccg cgcaacgatt cggcgagttc
     8341 gtcgacgcgc tcgacgaaaa ccggctcgag caagccgcgc gtgcgcactg cacggcaaat
     8401 ggcacgcccg tccgcgcccg ggatcggcgc ttctccggcg ccatacacct cggtgaccag
     8461 cagcacatcg cagccgccga gtacgcggcc gaagtcgtcc atcaagtcgc gtgtgcgggt
     8521 gtagcggtgc ggttgaaacg ccagcaccaa gcgacgcgac ggccaggcct ggcgcaccgc
     8581 atccaaggtc gccgcaacct cggtcggatg atgggcatag tcatccacca acagcgcctt
     8641 gccgccctgc cattggatct cgcccaactg ctgcaggcgc cggtcgatgc cctgaaaatt
     8701 cgcgagcgca cgctgaatgg cgtccgcagg aatatcgagc tcggtcgcaa cggcgacggc
     8761 cgccagcgaa ttgagcacat tgtggcggcc gggcaggttg atcgtgatcg gcaacggccc
     8821 gtgacccgcc cgcagcgctt ggtagcgcga ttgcaggccg tcacgcacca cctcgaccgc
     8881 gcggatgtcg gcctccggtg cgaacccata ggtcacgaac ggccgcgcca cattctgcaa
     8941 aatgctgcac acctgctcat cgtcgccgca caacaccgcc aggccgtaga acggcaggtt
     9001 gtgcaggaat tcgacgaagc tctgcttgag ccgcgagaag tcgccgtcgt gcgtcgtcaa
     9061 atgatcgttg tcgatattgg tgacgatggc gatcatcggc tgtaggtgca tgaacgaagc
     9121 gtcgctctcg tccgcctcgg cgacgagata tcggccggca ccgagtttgg cgttgctgtc
     9181 ggtgctcttc aaacggccgc cgatgacgaa ggtaggatcg aagcccgctt cggccagcac
     9241 gctcgcgacc aaactggtgg tggtggtttt gccgtgggtg cccgcgacgg cgatcgagta
     9301 gcggaagcgc atgagctcgc cgagcatttc cgcacgctgc accaccggga tgcgcttggc
     9361 ttgggcagcc acaacttcgg gattggagcg gctgaccgcg gtcgatacga ccaccacatc
     9421 ggattcgccc agatgcagtg cggaatggcc tatgaaaata tgcgcgccca ggcgtgccag
     9481 gcgctgcgta acggaatttt ccttgagatc cgagccctgc acggcgtagc ccagattgag
     9541 cagtacttcg gcgatacctc ccatgccgct gccgcctatg ccaaccaggt gaatgcgatg
     9601 aatacggcgc atgcggtcgt ccatgcgccg gcgctcgacc gggttcgggc gttcgctcat
     9661 gcgtgcgctc cggcggcaac gcacaggtca gccaggcgcg tcgcggcgtc gatgacgcgc
     9721 gaggcgcgcg cggcttgcgc catcttcagc aattggttgc ggtctgcgac cagccggctg
     9781 atcgcagagc ccagacgctc cggcgtcaga tcgcgttctt gaaggagctg cgcggcgccc
     9841 gacttcacca tgacttcggc gttctttgtc tggtgatcgt cggtggccac cggcaacggt
     9901 acgaagatcg cgcccaagcc tgcggcctgc agctcggcga cggtcatcgc gcccgcgcgg
     9961 cacaccgcca gatccgccca tgcgtaggct gatgccatat catcgatgaa gggcatcacc
    10021 tcggcctcga cctctgcctc tgcataggcc gcgcgcgccg cttccaggcc gcgctcgccg
    10081 gtctgatggc gcacctgcgg ccggatctcc ggagcgacgc gggcaagcgc ctccggaacc
    10141 atggcattga gacgcaccgc gccctgactg ccgccgaaca ccaacagccg cgagcgggtg
    10201 ttgcgctgcg caaaacgcag ctccggcggc gccaccgccg cgatatcggc gcgcaccgga
    10261 ttgccgatgg cgacggcatg aactcggggc gagaagctgc ccggaaatgc ctcgagcaca
    10321 ttgctcgcaa agcgcgacag catccggttg gtcattccgg cgatggcatt ttgttcgtga
    10381 atcagcaacg gaatccgcaa caaccaggca gcgactccgc ccggaccgct gacgtagccg
    10441 cccgccccca acacggactg aggccgcacg cgccgcaaaa ccccgattgc ctgcaccacc
    10501 gccttggcga cgcgccatgg ggcgagcagc cacgtcttga ggcccttgcc gcgaaccccg
    10561 ccgacccgta cccactcgat cgggaagccg ttggcgggca ccagccgcga ctccatgctg
    10621 cccggaacac ccagccaaac gaccccgatg ccgcgctcgc gcagcacctt ggccaccgcg
    10681 agcgccggga acacatgacc tccggtgccg cccgccatga tgagtacggg gccgcgcgcc
    10741 acttgcgttg cgggggcgct catcgaacac cgcccggcag gcgcatcacc gcggatcgcg
    10801 agctgcactt gacctcgtga aatatccgca gcactgcacc gagccacgcc aggctcacca
    10861 gcaggctgct gcggccgtag ctgagcagcg gcaaggtcag gcctttggtc ggcagcactc
    10921 ccatgttgac gccgatgttc accatggctt gcagtcccag ccatacaccg aaggcaagtg
    10981 ccaaataaga ctggaatctg aggccggcct gagccgccat ctgcgagatt ttgaatgccc
    11041 gccacaccag cgccatgaac agggcgatca cgccgagcac gccgaccagc ccaagttcct
    11101 cggccagcac ggcgaacacg aaatcggtgt gcgcctcggg caaataaaac aatttctgca
    11161 cgctgctgcc gagccccacc ccgaaccacg cgccgcggcc gatggcgatc agcgattgcg
    11221 tcaactgaaa gccgccgttg aacggatcgt cccagggatg caagaaaccc gtgagccgtt
    11281 tcaggcgata ggtcgaggtc agcgccagca cgctgaatgc gagcgtggcc gccgccacca
    11341 gcaacagcat gtaacgcagc cttgcaccgg ctacgaacaa caccgcaatg ccggtggcga
    11401 acagcaccgt ggtcgcgccg aagtcgggct cgcacagcaa cagcagtgca gcgaccgcca
    11461 agaggcccac cggcttggcc aagcccgaca gggcaatctg cagctccgtg cgcttgcgca
    11521 cgcagtagct gcacacccac gtcagcacca gcaccttggc gagttccgac acttgaaaat
    11581 tgaccggccc gacacgcagc cagcggcgtg caccattcac cgtcgcgccc actcccggaa
    11641 tcagcaccag gaacaacagc aacaatccgc aacacagcag caccaggctg tatttgtccc
    11701 acacctcggt cggcacgcgc gtgatgatcc aggcgaacgc gattccggcg aggccgaaga
    11761 tgaactggcg ctcgaggaag tagaaaggat tgccgagatc gcgcgcagcg atcgacatcg
    11821 aggccgaggt aaccatgatc aacccgacca acaacaggct ggccgccaac cccaggctga
    11881 ggtaatccca ggcaaagggc atgcgttcgc gcgcccgcac tccataggac atggtggcgg
    11941 cggtcatgtc gggcgctctt tcaacgtatg caccgcgacg gcgaacgcgt cgccgcgatc
    12001 ggcataatcg cgaaacatgt cgaagctcgc gcacgccggc gacagcagca ccgcatcgcc
    12061 cgctttggca acgcgcgccg ccaatgccac cgcatcctgc atggagccga ccgtgtcggt
    12121 gggacacacg cccgagagcg ccgcggcaat cgcctgcgca tccctgccga tcaataccgc
    12181 atgccgcacc ttgccgcgaa acgccgccgc cagcggcgtg aagtcctgca gtttgccctc
    12241 gcctccggcg atcattacca agggcccctg catgccggca acggcggcga tggttgcgcc
    12301 gacgttcgtg cccttcgagt cgtcgatgta gcgcacaccg tccacatccg cgatccactc
    12361 cgaacggtgc gcgaggcccg ggaaactctc gagcgcttcc agcatgctcg gcaccggcaa
    12421 acccaccgca tcgccgagag ccagcgccgc gagtgcattg gcggcgttgt gcagaccgga
    12481 aatcttcatg cggtcgatgt ccatcagcgc aacgccgcgg cgcgtcagaa atgtctgcga
    12541 gtcgatgcgc cgcagcgtga aatcggcatg ctcgctttca atcgaaaaag tcacgctgcg
    12601 cttgggcccc gcgccgcgca tcgcggcgac cagcggatcg tcgatgttca gcaccatggt
    12661 ttgcgcgttg ctgaagatcc gcgacttggc tgccgcatac gccgcgatcg acggataccg
    12721 gtccatgtgg tcaggcgtca cgttgagcac caccgccgcc gtaagctcga gcgaggtggt
    12781 ggtctccaat tgaaaactcg acagttccag gacgtacaag tccggcgtcg gttgatccag
    12841 caggtcgagc gcggcaacgc ccaggttgcc gccggcgagc acgttgcgtc cggcggcggc
    12901 ggccatgcgg gccaccaggc tggtcaccgt gctcttgccg ttggtgcccg tgaccccaat
    12961 gaccggcgcc gtcacgttgc gcgcgaacaa ttcgacgtcg ccgacgactt ctatgccgcg
    13021 atcgcgcgcc gctcgcgcga tcggctcctt gagcgaaacc cccggggaga tcagcacctg
    13081 cgatgcgccg tcgagcagcg acagatcgaa accgccgagc ctcaggtccg ccgatttcgc
    13141 gagctcgccc agctcggcaa gccccggcgg aacgggacgc gagtccgtgg cgctcgccgc
    13201 gataccgtgc cgcgacagat agcgcaggca ggaagcaccg gttttgccga gaccgacgac
    13261 gacactgtgc atcagcgtaa cttcaaggtt gcaagaccgg tcaatacgag cacgaaactg
    13321 atgatccaga aacgtacgat caccttcggc tcggcccagc ctttgagttc gaaatggtga
    13381 tgaatcggcg ccatgcggaa tattcgtttg ccggtgagcc tgaatgaggc gacctgcagc
    13441 atgaccgaga cggtctcgag tacgaatacg ccgcccatga cgaacagcac gatttcctgg
    13501 cgcacgatca ccgccagggt gccgagcgaa gcgccgattg cgagcgcgcc gacatcgccc
    13561 atgaagacct gcgcgggata ggagttgaac cacaggaatc caaggcccgc gcccaccagc
    13621 gtcgcgctga atatgagcgc ctcgccgacg cccgggatgt agggaatctg caaataggtc
    13681 gcaaatttga aattgcccgt cacgtaagcg aagacgccga gcgccgccgc caccatcacc
    13741 gccggcatga tggcgagacc atcgagtccg tcggtcaagt tcacggcgtt gctggtgccg
    13801 acgatgacga agtagcacaa cgcgacgtag gccgcggtcg acatggggac cgccacggtc
    13861 ttgaagaatg gcacgtacag cgaggtctcg gcgggcgaca ggcgcaggtg atgcagcgtc
    13921 atgatggtcg cgatgccgac cagcgattgc gccagatatt tatagcgcgc tgccaggccc
    13981 ttcgagttgc cgacgaccaa tttcaaataa tcgtcgtaga agcctatcaa cccgaaaccc
    14041 aaggtggttg ccaacaagat ccagacgaag cggctcgaca agtcggccca caacagggta
    14101 cccaggacca tggcgatcaa aatgagacct ccgcccatgg tcggcgtgcc ggatttctgc
    14161 aagtgcgact gcggtccgtc gctgcgcacc tgctgcccga tctgatagcg gctcagcatc
    14221 gcgatcatgg tcggtccgat gaaaagcgcc agcgcgagcg cggtgaccgc cgcgagaatg
    14281 ccgcgcagcg tgaggtactg gaagacatga aagccggtgt agaaccgcgc cagatactcg
    14341 gtgagagcca gcaacatggc gtcagttcgc tccggtgagc gcttgcacca cgcgctcgag
    14401 tcgattcacc cgggaaccct tgaccaacac ggtgactccc ggcgcgattt cggcctgcag
    14461 gcggcgagtc agagaatcgg catccgcgaa ccactcggcc ccggagccga aggtctcgac
    14521 ggcgcgcgaa ctttgcggtc ctacggcgaa caggcgcttg atcccgcagt cgcgagcgta
    14581 tgcgcccatg tgtgcgtggc tgtcctcggc aaattcgccg agctccgcca tatcgccgag
    14641 caccagccaa gtggcgccgg gcagggcgcg cagcacctcg agtccggcgc gcaccgaact
    14701 cggattggcg ttgtatgagt catcgatgat ccaactgtcg cgcgcgccgg ctttgagctg
    14761 cagacgcccg gcgaccgcgc gaaaatccgc caatccgagc gcgatttcct cgagcgatgc
    14821 gccggcggcg ccggcggcgg cggcggccgc cagcgcgttg ccgatgttgt gcgcgccgcc
    14881 ggccttgagc gtgatcgggt gctgccccaa gggacagcgt agagtgaagc gggtcaggaa
    14941 ctctccgcgc tcgatcgtct gcttggaatc ttgcgccgtg aaatccgcgg cagagtgcac
    15001 gccgaaggtc acgatgcggc gcgccgtcgc gacgctgcgc caataacccg cgtaggcgtc
    15061 gtcggcgttg atcaccgccg tcgccgccgg ctcgagaccg gacaccattt cgccctcgcc
    15121 ttgagcgaca ccgtcgagag tgcggaatcc ctccaggtgt tcggcgccgg cgttggtgat
    15181 gagcccgacg gtcgggcgcg ccaggcgcac cagctcggcg acatcgccga tgcgattcgc
    15241 gcccatctcg atgacggcgc tgcgatgcga tgcctcgagg cgcatcagcg tcaggggcac
    15301 gccgatgtgg ttattcaggt tgccgtgcgt cgccataacg acgcccatgc gcgacaagat
    15361 ggcggccgtc atttccttgg tggtggtctt gccgttgctg ccgcccacgg ccaccaccgg
    15421 cacatcgaag ccggcgcgcc atgcctggcc cagctgctgc agagcgcgca gtgcatccgc
    15481 caccacgatc tgcggcacgg cgtgcgagac cgcgcgttca acgatggcgc cgatggcgcc
    15541 gcgcgtcgcc gccgccgtaa cgaagtctcg gccatcgaat ttggggccgc gcaacgcggc
    15601 gaacagcgtt cccggagcga gcgttcgact gtccgtcgaa actgcgccga acggacgatc
    15661 ctcgccgatc aattgaccgc cgaccgccgt ggctgcctca tgcaaggtgc gcttcatgcc
    15721 gccaccccga gatggcgccg agcttcgagc cgatcgctga agctgcgccg cgtatcgccg
    15781 taaatttgat agtcctcgtg acccttgccg gcgatcagaa ccacgtccgt cgcgcgcgcc
    15841 tctttgagcg cggtgccgat ggcctcgccc cggtcgtgga tgacgcgtac cgtgcgcgac
    15901 ttgatgccgc tgatgatggc gccggttatc gcttgcggat cttcggagcg cgggttgtca
    15961 tcggtaacga tgactcgatc ggccaattca tcggcgatgc tgcccatgag ggggcgcttg
    16021 ccgggatcgc ggtcgccgcc gcaaccgaag acgcaccaca gcgagcctgc gcaatgctcg
    16081 cgcagcgcgc ccaaggcctt cgccaatgcg tcgggcgtat gtgcgtagtc caccaccgcc
    16141 agcggcttgc cgcgagcgtc ggggtcgacg acttccatgc gtccgggagg cgggttgcat
    16201 tcggcaagca cccgagcagc ctcggcgagc gatgcgccga gcgagagcag acaggcgatg
    16261 acaatcagag aattatcggc gttgaagcgg ccgagcaagc gggtgtcgac ccgggcctcg
    16321 ccgaagctgc cgtcgagctg catcgaaacg ccgtgcacat ccagagtcac ttgggtcgcg
    16381 tgcagcgtgc gccgcgtcgg cagcgtatcg ccggcgccga tccacacggc ggtgagcggt
    16441 gcccgtccgg cgtagttctg tgccaactcg cgcccgaagg catcggcgac gttgacgacg
    16501 atgtgctcga ggtccggcgc catgaacagc cgtgccttgg cggccccgta cgcggtcatg
    16561 gtctggtgat aatccaaatg atcgcggctc aagttggtga atgcggccgc gtggaagcgc
    16621 actgcggcga cgcgatcttg atcgagagcc tgcgacgaca cttccatcgc cacgtcgcgc
    16681 acaccggcgg catggagctc ggccagttcg cggtgcaatg tgacggcgtc gggcgtggtg
    16741 aggcttgctg gtgcgagcga cgcgattcgg ccccatccga gagtgccgat gtaggcggct
    16801 cgggacccca agcgctccag acattgcgcc agcaaaaagg cgcaggtggt cttgccgttg
    16861 gttccggtga ttcccgtgat gcgcagctgc gccgaaggcg acttgaaaaa gcgatcggca
    16921 atacgcccaa ccaaagccct caaattcgga atcgccgccg cgaataccct cgccggcaat
    16981 gccggcaact cgatcccggc cgccggttcc cacagcacta cgctggcgcc gcgtgcggcg
    17041 gcttcggcgg cgaacttgac gccatgcacg ctgcggccgc gcaacgcaag aaatagactg
    17101 ccggcgcgca cctcgcggct gtcgagcgtg agatcgtgca cttcgcagcg cgcggcgtcc
    17161 gggagcttcg cgatgccttc gagcagccac atgagtgaga cgctcatggt gtttcgttgg
    17221 cctgcaccag cgtcgcagcc gggacgtttt gcaaatcgtc gggcggcacg tccatcagcc
    17281 gcagggcgcc ggacatgacg cttgcgaaca ccggcgcggc caccgtgccg ccctgatgca
    17341 aatcgccgct cggctcgtcg atcaccacca ccgtcgcgag tttcgggtgc gatgccggca
    17401 ccaggccggc aaagacggcc atataacgat ccgtcgagta gcccccggca atcgatttcc
    17461 aggcggtacc ggtttttccg gatacgcggt agccgatgag cgaggcgcgg gtcgcggtgc
    17521 cgcccttttc gaccacctgc tccatcagct gcaccaacgt cgcgcacacc ttggaatcga
    17581 gaacgcgctc gccgggcacg ggtccttgca cgcgctcgaa agaaatgggc cgcttgatgc
    17641 ccccggcgcc gatggtcgcg taggcatgcg ccagctgcag gggcgtgacc gacagtccat
    17701 aaccgtgcga cagcgtcgcg atgccgatcg gccgccaatg cgaatagtga ctgagcattc
    17761 cggccgattc gcccggataa ccgctgcccg tcacctggcc caatcccaag cccgtcaagg
    17821 tgctccagat ttgctcgggt tgcagagaca aagcgacctt ggcggtgccg acgttgctcg
    17881 acttcgccaa gatcgtcgcc agatcaatgg cgcccaagtt ctgctcgtcc tcgaacactt
    17941 tggtgccgac tttgaggaaa ccgggcgagg tatccaccac gctgtcggcg cggtactgcc
    18001 ccgaggccag agccgcggcc atgatgaagg gtttgatgct cgaaccgggt tcgaaaatgt
    18061 cggtggcagc gcgattgcgg tacagacccg ctttgagctg ctcgcggtcg ttggggttgt
    18121 aggccggctg attgaccatg gcgagcactt caccggtatc gacgtcgatg acgatgacgg
    18181 agccggcgcg cgcccggtac tcttgcattg ccgccttcaa ttcgcgatag gcgagatatt
    18241 gaatgcgcag atcgatactg gtgagaagat cccggcccgg ctgcggcgca cggatgcttt
    18301 cgacattctc gacgtagcgt ccgtacatat cctgaatgac gcgcttcgcg ccgtcttcgc
    18361 cgttcagcaa ctgatcgaag ccgagctcga gtccctcctg tcccttgtcg tcgatggtgg
    18421 tgaagccgac gacatggccg gagatctcgc cggcgggata gtagcgccgg tattcgcgca
    18481 gcaagtacac gccggggatg ccgagcgcct tgatgtgcgc ggcctgttcg ggcggcatgt
    18541 ggcgctccaa gtagagaaac tcacggtcca aattgctggt gatgcgccgc gccagcgact
    18601 gttgatcctg cttcagtgcc tttgcgagct tcggcagctc atcgatgttg tcattgagct
    18661 cctgcgggtt gacccaaacg ctgtcgaccg gcgtcgagac cgcgagcggc tcgccgttcc
    18721 tatccgtgat cgcgccgcga tgcgcggcga tcttgaccac gcgcatcgag cggtcgtcgc
    18781 cttgcttcgc gagaaagccg tgatccagca actgcagctc gactgcgcgc gccacgagcc
    18841 ccaccgcacc gaccatcagc aggcccagga tcgcgctcga acgccagcga tagctccttg
    18901 cctgagtgtc gttgtcgccg ctccgcaact tcacggccgc acgatccgta cgtcgcgcgt
    18961 ctgcggcaag ttcatttgca gcttggcgtt ggcgactcgc tcgacgaagg cgtgcgagga
    19021 ccaatagctc tgttcgagct gcagacggcc ccactccata tcgagcgaat cgcgatcacc
    19081 attgagcttc tccaagtgca cgaacatgtc gcgggccttg tgccgtgcat agatcacttg
    19141 caccgcggta ccgagcaccg cgagccacaa cacgggcacg gcgagcgaca accaactgcg
    19201 gtgtttcatg cggccgctcg ttgcggcaag cgttcggcga cgcgcagacg cgcgctgcgt
    19261 gcgcgcggat tgcgaaacgc ttcgttcgcg tccggctcga tggatttgcc gacgagcttg
    19321 agtgtcgggc gggcctgcgg cgggatattc ggcaaccccg catacatggg gtccgccatc
    19381 gcgtggcggc gcatgaattg cttgaccaag cggtcctcga gcgaatgaaa gctgatcacg
    19441 gccaggcgtc cggcgggcgc gagacgctcg agcgcggcat tgagcccggc ctcgagctcg
    19501 tcgagttcac cgttcacgtg catgcgcaat gcctgaaata cgcgggtcgc cgggtgcttg
    19561 tgcacgggtc gacccgttgc gtcgtaccgc ggagcgactc ttgcgatcat ttcagcgagc
    19621 tgcagggttc gttcgaaggg ctgcgattcg cggtctttga cgatggcgcg cgcgatgcgc
    19681 gctgcctggc gctcctcgcc gtagtcgcgg aagattcgaa tcaactcatg cagcgacgcg
    19741 ccgttgacga cgtccgccgc gctgaagccg ccctgcgccg tcatgcgcat atcgagcgga
    19801 ccgtcttgca tgaagctgaa gccgcgcgcc gcatcatcga gctgcggcga ggacacaccc
    19861 aaatcaaaca aaattccgtc gaacttgagc cccgcttcga cctggtccgc gcactgcgcc
    19921 agcgccgaga agcgcgcaag aaacagcgtc acgcgccggt ccgccgcaaa gcgcgacttg
    19981 gcgtccgcaa tggcggccga atcgcgatcg aggcatagca aacggccgtt cgcgaagagg
    20041 ttgtgcaaaa tcgcccctgc atgtccgcct cgaccgaaag tggcatccaa ataggtgccg
    20101 cttccccgta tgttcaacgc ggccagtact tgaggtaaca gtaccggcgt gtgaactccc
    20161 gcaatgtctt gcaccgcgcc cgccggctac agcgacaacg tttcaagttc tgtaggtaaa
    20221 tccgtggtgc tgccatcgcc cgcgagccac tcgtcgcggc gctcgttcca ccgcgcctca
    20281 tcccacaatt cgaaatacaa accctgacca atgagcatcg cgtcgcgcgt caacaggctg
    20341 aattcgcgca aattcggcgg caacaacacc cgtccatggc catccaattc cagatccgtg
    20401 gcgtgtccca ccatcagccg ctgcagccgc cgcgactggg ggttcatgga cggtaaacgc
    20461 atcaatttgc gctcgatctc ttcccaatcc ggcagcgggt agatgagcag gcactgatcc
    20521 ttgtcaacag tgaccaccaa tttgccccca caacgctcct gcagccgctc gcgataccgg
    20581 gtgggcatca ccacgcggcc tttcacatcg agggttagtt tgttggcgcc gcgaaacata
    20641 attggatggg tctggcgacc cattctctcc cacaatttcc caccgggcac cactataggg
    20701 agcgcttgga cgcactgtca acgttttaag ttaaaaaaaa cccagcccga cagtgactta
    20761 ggtcacattt tgtacagcgt cgcagagtga cgacagtttg tttgtttaga ataaaatctg
    20821 ccggacgctc ttccggtagg ggcacaaaaa gactcaaagg cggggatctt cgtcaattct
    20881 cggagatctt gggtcgatcc tccgtttttg gtccacggcc ggctggatcg gtcaagtaag
    20941 ccgacgctct ggccatgctg gccaggctca acgaaaaacc tgatgcagat cagcagcgac
    21001 gcgtgcgaac aggccctgcc agtccccgag agcgtgctgt cggtacagcg tgacgcttgg
    21061 ataccacgga ctatcgattc tgcccacgag ccagcgccag tccgcagcga agggcagcat
    21121 gacccacgta ttcctgccga gaccggcgct tagatgagcg atgctggtgc aaacgctgat
    21181 cacgagatct acgcattcgc acaatgccgc cgtctcggtg aaatcagggt cgaatgtcga
    21241 aatccagcgg ttcgcctcca ggacttcccg gtcggccggg cggatttccc tctgcagaca
    21301 aatgtattga aaatctcgtg gcagatgctc gatccagtcc aacaggcgaa cgctgcgatt
    21361 gtgatcacga ccattttttg gattgccact ccagaccagc ccgattcgcg gaccgttccg
    21421 cggtcccaat cggctctccc actcggggat cttggacgct tggctgtgca ggtatcttct
    21481 cggagcgggt attgtgtcga gggtggtttt cagtgccagc ggtaaactga gcaacgggca
    21541 atgataatca aacgggggta gcaaaccgcc ttccgggact accagcgata ctccatcgag
    21601 gtccttcaac aaactcacca acggagcctg cacctcgaga atcaccgttg cgccgcgctc
    21661 ggcgaccgtc ttcgcgaagc ggcaaaactg caatgtatcg cctagccctt gctcgcaata
    21721 tagcagcagc gtttttccga atatttcctc ctcgcccagc caaagaggtt gcttgaacga
    21781 gcgccattct gtctgtaatc gttcggcatt tttccaccgc cactcgtgca gacgccagcc
    21841 ttcttcatag tcgccgtgca acaacaacgc cagcgaccta ttgagattcg cttccgcgaa
    21901 gtcgggccga atcgtcgtcg cccgctcgta gtcgacgagc gcctcgttca cacgtccaac
    21961 ttccaacagc accccggcac ggtcgcaata ggcctccgca taatcaggtc tcagggcgat
    22021 cgctcgatcg taactggcga gcgccgcctc gaactgcata ttcgcctgaa ggagagtacc
    22081 gcggttgaaa tgggcttccg ggtagttgca gcggatcgag atggcgcggt cgcaactggc
    22141 caacgcttcg tcccgacgcc caagctcctg taacagcacg ccccgattgc tatgggcctc
    22201 ggcatagtca ggcttggcgg caatggcacg gtcatagctg gccagagccg cctcgcgctc
    22261 gccgagttgc ttcagcacgc cggcgcgatt gaagtgcgcc tcatgaaatt cagggtagat
    22321 cgacaccgcc cgctcgtaac ttgacagcgc gtctcgatat cgcccgcatc gttccaggag
    22381 gactgcgcag ttgtaatggc ctgcggcatg gtcgggctgc aacttgaccg cctgctcata
    22441 actgctgagc gcctcggtca gacgccgcat ttgccgaagc acattaccgc gatggaacca
    22501 aacttgaaac agttgcggat tcagtgcgag cgcctgatcg t
//
LOCUS       DASWZT010000555         9410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_38440, whole genome shotgun sequence.
ACCESSION   DASWZT010000555 DASWZT010000000
VERSION     DASWZT010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9410
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38440"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VGE92_15910"
     CDS             <1..672
                     /locus_tag="VGE92_15910"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEY1315368.1"
                     /translation="FAVRLSRVLVPAELTDPMSGFFMMRRSVLDGAARNLSAIGFKIL
                     TDVFASCPQPLRFKELAYRFRVRRAGQSKLDSVTAWDYLMLLLDKTIGRFIPVRFLAF
                     SIVGAVGVALHFAVLTLIFQGLHRSFVTGQAVATFGAMSFNFAVNNVLTYRDRRLRGV
                     RWLRGWASFVLACSIGGFANLGVASTVYGLGHGWLPAAVAGILVGAVWNYAVTMMLTW
                     GRSRR"
     gene            complement(679..915)
                     /locus_tag="VGE92_15915"
     CDS             complement(679..915)
                     /locus_tag="VGE92_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315369.1"
                     /translation="MSPKAILFSIGCALAWFVLLPVLVVGGALTLFSYAIFAEVASLL
                     SGKPTETVDSAAAREIARRMCGYAVERRRTRRHT"
     gene            complement(1137..2591)
                     /locus_tag="VGE92_15920"
     CDS             complement(1137..2591)
                     /locus_tag="VGE92_15920"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY1315370.1"
                     /translation="MIRSSAVSLALAYVALGITALVLFAAPLWYAWRVTIQEGRQEIL
                     QADAQRLTDVYRREGAGGLKSFIDTRVGMQIAGDRILLLTDSSLHPLAGNLAMWPASV
                     PAKAGNYVLQVAVGNQPPEPALVHVAVVGNQILLVGRYNTIFASLQTHFWYGLAAAIA
                     VLSIAGLLVGVLTRRALMSRVDSIRQTVSAIIHGDLQHRLPTQLQDDELNTLSRTING
                     MLEQIELLVHGVRNVSNSIAHDLRTPLAELRSRLEELALIRPPPEQAFAEIDGAVADV
                     DRVIRIFDALLRLAEIDAGMRRSGFVALDISDLAANAVEFYAPAAELKNIHLGFRSDG
                     PLSVSGDPVLLAQALSNLIDNSLKYAPVNGVIEVAVLKQGDGHVEVSVTDNGPGITDS
                     EKSKVVERFYRGDASRGTPGVGLGLSLVQAVAKLHGSALALSDQSPGLRVALSLALET
                     SIAQAAAAPQASATRTTARLTPAAELPPYPQRSN"
     gene            complement(2593..3273)
                     /locus_tag="VGE92_15925"
     CDS             complement(2593..3273)
                     /locus_tag="VGE92_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY1315371.1"
                     /translation="MKILLVEDNERVSQFVIKGLQEAGHTTDHAGNGRDGMFLAASEP
                     YDVIIMDRMLPGEIDGLAIVEVLRKAGKRTPILILSALSEVDDRIRGLRSGGDDYLTK
                     PFASGELLARLDALHRRAQAVNTEIPLTVGDLHMDPLTRKVTRGNRPITLQPREFKLL
                     EYLMRHAGQVVTRTMLLENVWDYHFDPQTNVVDVHISKLRQKIDAEFERPLLRTVRNA
                     GYTVTAGD"
     gene            complement(3270..6389)
                     /locus_tag="VGE92_15930"
     CDS             complement(3270..6389)
                     /locus_tag="VGE92_15930"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1315372.1"
                     /translation="MSISEPFVRRPIATSLLMGGIFLVGLVVYPLLPVAPLPQVDFPT
                     ILVTAQLPGGSPETMAASVATPLEYQFAEISGLAQMTSTNVYGTTQITLQFDLNRNID
                     AAAQDVQTGIDAAGGQLPKNLPTPPTYKKVNPADSPILILAVHSDVLPVTTVDDYTEN
                     ILAQQISQIPGVAQVSIGGQQKPAVRVQIDPTRLAALGLQMEDVAGVITAATVDAPKG
                     SINGPKRSMAIYDNDQLLKAAPWNDVVVAYKNGAAVRIRDIGVAVDGPENTLLTAWQN
                     GHNGILLLIYKQPGANVIDAEQRVEALLPHALASVPPSIKVDTVANRTTTIKAAVRDV
                     EFTLALTIALVVLVIFLFLRNAWATIIPGVTVPLALLGTAAVMYVLNYSLDNLSLMAL
                     TIAVGFVVDDAIVMLENVYRHLEAGLSPLEATLKGAGEIGFTIMSISVSLVAVFIPLL
                     LMGGIIGRLFREFAITMTMTIVISAFVALTLSPTMCALFLRDEKHAKHGKAYMAIERA
                     FDRLLAGYTRGLDFCLDHRRATLTVFLITVAATVLLYIEIPKGFFPQQDTGIISGLSD
                     GPQDISFDEMVRRQHALMDVVAKDPDVESYGTGLGGNRPLNNGFVVIGLKPRDQRDSN
                     ADQIITRLRRQLTQVPGATLFLQAAQDLNVGGRTSRTQYQYTLQDSDINELNDWAPRL
                     LAVLQKLPMLRDVASDQQTSSGMLSLTIDRDQAARFGIQPAAIDQALYDAYGQRQAAQ
                     FFTQANSYHVILEITPSLQTDPSSLDKLYLKSALTGQMVPLSAMTKYDTQHVTYLSIN
                     HQGQFPAVTLSFNLAPGAALGDAVTAINRASATMHLPATITGNFQGTAQAFQSSLKSQ
                     PYLILAALVAVYIILGMLYESYIHPLTILSTLPSAGVGALLVLLIFHIDLSVMALIGI
                     ILLIGIVKKNGIMMVDFAIQAEREQHLSPHDSIRQACLLRFRPIMMTTMAALLGALPL
                     ALGHGTGSELRQPLGYTMVGGLVLSQALTLFTTPVIYLYLDELSLRFRRRRPEQRPEP
                     VVPPLEGAT"
     gene            complement(6386..7582)
                     /locus_tag="VGE92_15935"
     CDS             complement(6386..7582)
                     /locus_tag="VGE92_15935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY1315373.1"
                     /translation="MKGDVQMNDASTIRRYRCGWLGVLALAVVALIAWRVVVYRRAQA
                     AAHAPPPAIAVDTAVVAHSEVPIYLSGLGTVQAFYTVTVTARVDGELQKIAFTEGQTV
                     HKGDLLAQIDPRPNQAAFDQALATKAKDAAQLVNAKRDLERYTILQPQDLASKQTVDT
                     ARAMVDQLDAQLQVDQALIDNARTQLEYTRITSPIDGRTGIRLVDPGNIVHAAATTGI
                     VVVTQVQPISVIFTLPEEDLATVNTALAAGPVSVTTVARGGGTELDQGTLTLVDNEID
                     QPTGTAKLKATFANAHNTLWPGQFVNARVLVRTLHDALTMPSAAVQLGPNGPFTYVVK
                     PDSTVEVRQIKIGDDSGGLTVVNAGLALNEHVVTSNQYRLQAGVHVRAATAEAATRSP
                     QAANAS"
     gene            complement(7572..8984)
                     /locus_tag="VGE92_15940"
     CDS             complement(7572..8984)
                     /locus_tag="VGE92_15940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY1315374.1"
                     /translation="MRWHALGAALCVCGVAWLGACSVGPTYQRPNIPPPAQWHESAAA
                     DAGRPDAAAGVWPAADWWHGFGSARLDALIDEAERSNDDLAAAIARVQEADAQLRIAG
                     APLLPSLDLGATATRERAQVTGIGPRVFNDFNPQLNASYELDFWGKNRALRDAARAAA
                     IASRYDQQTIALTVISSVATTYFQALELRDRIRVAQQNLDNGRKILKGFQLEQTAGTA
                     TGLDVAQQETAVALLSAALPPLQQQLRQTVYALAVLIGKTPESVDVDTGTLQELSAPV
                     IVEGLPSQLLTRRPDVAEAEQQLIAANADITVARAALFPSIELTAGGGYESSALAALI
                     SPPNRIYALTAGLTQPIFHGGALRGQVAYSNARYTELLRTYHKTVLSAFSNVEGALVA
                     TQQTAEQQTRQQDAVTVARRAYDFALQQMSAGTVNILTVLNTENALFSAQDQLLQVNY
                     SHLQSLVDLFTALGGGWHEG"
     gene            9124..>9410
                     /locus_tag="VGE92_15945"
     CDS             9124..>9410
                     /locus_tag="VGE92_15945"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY1315375.1"
                     /translation="MVTTSAAESFDFCTLLTSTQDQMARALLERITGKWSLAVMRALA
                     EAGGPLRFSRVLERVSGISQKVLTQTLRTLERDGLISRTLYPQVPPRVEYEL"
BASE COUNT         1728 a         2823 c         3076 g         1783 t
ORIGIN      
        1 ttcgccgtgc ggctcagccg cgtgttggtt ccagcggaat tgaccgaccc catgagcgga
       61 ttcttcatga tgcgccgctc ggtgctggat ggcgcggcgc gcaatctctc ggcgatcggc
      121 ttcaagatcc tcaccgatgt attcgcgtcg tgtccgcagc cactacgctt caaggagctg
      181 gcgtatcgat tccgagtgcg ccgagcgggg cagagcaaac tcgattcggt aacggcgtgg
      241 gactacctca tgctgctgct cgacaaaacc atcggccgct tcatcccggt gcgtttcctt
      301 gcgttttcga tcgtcggcgc cgtgggcgtc gcgctgcact tcgctgtgct gacgctgatc
      361 tttcagggat tgcaccgcag tttcgtcacg ggtcaggcgg tggcgacgtt cggtgccatg
      421 tcgttcaact tcgccgtcaa caatgttctc acctaccgtg acaggcggct tcgtggagtg
      481 cgctggctgc gcggatgggc gtcgttcgtg cttgcctgca gtatcggcgg ctttgcgaat
      541 ttgggcgtgg catccaccgt ctatggactg ggtcacggct ggctgccggc ggcagtcgcc
      601 ggaatactcg tcggcgcggt gtggaactac gccgttacga tgatgctgac gtggggtcgt
      661 tcacgtcgct gagggcgttt aggtgtggcg tcgcgtcctg cgccgctcga ccgcatagcc
      721 gcacatgcgg cgggcaattt cgcgcgccgc ggcgctatcg actgtttcgg tgggcttgcc
      781 gctgagcaaa ctcgccacct cggcaaaaat cgcataggaa aacaaggtga gggcgccgcc
      841 gaccacgagg accggcaaca acacgaacca ggcaagcgcg caaccaatcg aaaagagaat
      901 ggctttcggc gacattgtgg ataacccttg ctgaacgttc actgccacta agaccaggtt
      961 gcagaataga cccgcaaggc ctcgaacaac cgagaactgg atcgcatttc tgagatcggc
     1021 ttgcaagcgg gtcaagatta agtaaaattc acaagctatt gtatataaac aacttttata
     1081 gccaaggcga catattcaga tgcgcagtgg gtacttatcg ttacctcgct tgcgcttcaa
     1141 ttcgagcgtt gcggataagg cggcagttct gcggccggcg tgagtcgcgc cgtggtacgt
     1201 gttgcgctcg cttgcggcgc tgcggccgct tgagcgatgc tggtttcgag cgccaggctg
     1261 agtgctacac gcaatcccgg gctttggtcc gacagcgcca aggcgctgcc gtgtagcttg
     1321 gcgaccgcct gcaccaaact caagccaagg ccgacaccgg gcgtcccgcg gctcgcatcg
     1381 ccacgataga agcgctccac gaccttgctt ttttccgagt ccgtgatgcc cggaccattg
     1441 tcggtgaccg acacctcgac gtggccgtcg ccctgcttca gcactgccac ttcaatcacg
     1501 ccgttcaccg gcgcatattt cagcgagttg tcgatcagat tgctcaaggc ctgggcgagc
     1561 agcaccggat ctcccgacac cgagagcggg ccgtccgatc gaaacccgag atggatgttc
     1621 ttgagctcgg ccgcaggggc atagaattcc accgcgttcg cagcgaggtc cgagatgtcg
     1681 agagcgacga aaccggagcg gcgcatgccg gcatcgattt cggcgagccg cagcagggca
     1741 tcgaagatac gaatgacgcg atccacatcc gccacggcgc catcgatttc cgcgaacgcc
     1801 tgctcgggcg gcgggcggat cagcgccaac tcctcgagtc gcgagcgcag ctccgcgagc
     1861 ggggtgcgaa ggtcgtgggc gatggaattg gacacgttgc gcacgccatg caccaagagc
     1921 tcgatctgct cgagcatgcc gttgatggtg cgcgacaagg tattgagttc atcgtcctgc
     1981 agctgcgtcg gcaagcggtg ctgcaaatca ccgtggatga tggccgatac ggtttgacgg
     2041 atgctgtcga cccgcgacat cagtgcgcgg cgggtaagga cgccgaccag cagccccgcg
     2101 atcgacagca ccgcgatcgc ggccgcaagg ccgtaccaga aatgcgtctg cagcgatgcg
     2161 aaaatcgtgt tgtagcggcc gaccagcagg atctggttgc cgaccacggc aacgtgcacc
     2221 agcgccggtt cggggggctg attgccgacc gcaacctgca gcacgtagtt accggccttg
     2281 gccggaacgc tcgccggcca catcgcaaga ttcccggcga gcggatgcag cgagctgtcg
     2341 gtcagcagca gaattcggtc gccggcgatc tgcatgccga cgcgggtgtc gatgaagctc
     2401 ttcaggccgc cggcgccctc gcggcgatag acatcggtga ggcgctgtgc gtcggcttgc
     2461 agaatttcct ggcggccctc ctggatcgtg acccgccagg cgtaccataa gggcgccgca
     2521 aacagcacca gcgcggtgat gccgagcgcc acataggcga gcgccaacga caccgcggat
     2581 gagcggatca ttctagtcac ccgcggtcac cgtgtagccg gcattgcgca cggtgcgcag
     2641 cagcggacgc tcgaactcgg cgtcgatctt ttgccgcaac ttgctgatgt gcacgtcgac
     2701 gacgttggtc tgcggatcga agtgataatc ccacacgttc tccagcagca tggtgcgagt
     2761 gaccacctgg ccggcatgac gcatcaagta ttcgagcagc ttgaattcgc ggggctgcag
     2821 cgtgatcggc cggttgccgc gagttacctt gcgcgtcaac gggtccatat gcaagtcgcc
     2881 gacggtgagc gggatctcgg tgttgacggc ttgagcgcgg cgatgcaaag cgtcgagacg
     2941 cgccagaagc tcgccggaag cgaacggctt ggtcaggtaa tcatcgccgc cgctgcgcag
     3001 tccgcggatg cggtcatcga cctccgagag cgcgctcaat atcaggatcg gcgtacgctt
     3061 gccggctttg cgcagaactt cgacgatggc gaggccgtcg atctcgcccg gcagcattcg
     3121 gtccatgatg atcacatcgt acggttcgct ggcggcgagg aacatgccgt cgcggccgtt
     3181 gcccgcgtga tccgtggtgt gtccggcttc ctgcagaccc ttgatgacga actggctgac
     3241 ccgctcgttg tcctcgacca gcaaaatctt cacgtcgcgc cttcgagcgg cggcaccacg
     3301 ggctcgggcc gctgctcggg ccggcggcgc ctgaaccgca ggctgagttc gtccaagtaa
     3361 agatagatga cgggcgtggt gaacagcgtg agtgcctggc tcaacaccaa tcccccgacc
     3421 atcgtgtagc cgagcggctg gcgcagctcc gagcccgtgc cgtgtccgag tgccagcggc
     3481 agcgcgccga gcaacgcagc catcgtcgtc atcatgatcg ggcgaaagcg cagcagacat
     3541 gcctgccgga tcgaatcgtg cggcgagagg tgctgctcgc gctcggcttg aatggcgaag
     3601 tccaccatca tgatgccgtt tttcttcacg ataccgatca gcaaaatgat tccgatcagc
     3661 gccatgacgc tcaaatcgat atgaaaaatc aacagcacga gcagcgcgcc gacgcccgcc
     3721 gagggcagcg tcgagagaat cgtcagcgga tgaatgtaac tttcgtacag catgccgaga
     3781 atgatgtaaa cggccaccag cgcggccagg atcaagtacg gctggctctt caacgagctc
     3841 tgaaaggctt gcgcggtgcc ttggaagttg ccggtgatcg tggccggcag atgcatggtc
     3901 gccgatgcgc gattgatggc ggtcacggcg tcgccgagag cggcgcccgg cgccagattg
     3961 aacgacaggg tcaccgccgg aaactgtccc tgatgattga tggataggta ggtgacgtgc
     4021 tgggtatcgt acttggtcat cgccgacagc ggcaccatct ggcccgtgag cgctgatttc
     4081 aaatacagct tgtcgagcga ggacggatcg gtctgcagcg acggtgtgat ctccaaaatg
     4141 acgtgatagc tattggcctg cgtaaagaat tgcgccgcct ggcgttggcc gtaggcatcg
     4201 tacagcgcct gatcgatggc ggccggttga ataccgaagc gcgctgcctg atcgcggtcg
     4261 atggtcaggg acaacatgcc gctgctggtt tgttgatcgg aggccacatc acgcagcatc
     4321 ggcagcttct gcagcaccgc gagcagccgc ggcgcccagt cgttgagttc gttgatgtcc
     4381 gagtcctgaa gcgtgtattg atactgcgtg cgactggtgc ggccgcccac attcaagtcc
     4441 tgcgcagcct gcaagaacag cgtcgccccc gggacctggg tcagctggcg gcgcagccgt
     4501 gtgatgatct ggtcggcgtt cgagtcgcgt tgatcgcgag gcttcaatcc gatcacgaca
     4561 aagccgttgt tgagaggccg gtttccgcca agaccggtgc cgtaactttc gacgtccgga
     4621 tccttcgcga ccacatccat cagggcgtgc tgccggcgca ccatttcgtc gaatgaaata
     4681 tcctgcgggc cgtccgagag tccggaaatg atgccggtgt cttgctgcgg gaagaaccct
     4741 ttggggatct cgatgtacag cagcaccgtt gccgcgaccg tgatcaggaa aaccgtcagg
     4801 gtggcgcgcc tgtggtcgag gcagaaatcc aggccgcgcg tatagccggc gagcagtcga
     4861 tcgaaagccc tctcgatcgc catgtaggcc ttgccgtgct tggcgtgttt ctcgtcgcgc
     4921 aggaacaggg cacacatggt cggcgacaac gtcagcgcca cgaatgccga gatgacgatg
     4981 gtcatcgtca tggtgatggc gaactcgcgg aacagccggc cgatgatgcc gcccatcagc
     5041 agcaagggaa tgaacacggc gaccaaggag acgctgatcg acatgatggt gaagccgatc
     5101 tcgcccgcgc ccttgagcgt ggcctcgagc ggcgacagac ccgcttcgag gtgccgatag
     5161 acgttctcga gcatgacgat ggcatcgtcg accacgaagc cgaccgcgat ggtcaaggcc
     5221 atcaaagaga gattgtcgag actgtaattg agcacgtaca tcactgccgc agtgccgagc
     5281 agcgccagcg gcaccgtgac gccggggatg atggttgccc aggcgttgcg taggaacagg
     5341 aaaatcacca gcaccaccaa ggcgatggtg agcgccaggg tgaattccac atcgcgcacc
     5401 gcggctttga tggtggtggt gcgattggcc accgtgtcca ccttgatcga cggcggcacc
     5461 gaggcaagtg catgcggcag cagcgcttcg acgcgctgct cggcatcgat gacattggcg
     5521 cccggctgtt tgtagatcaa cagcagaatg ccgttgtggc cgttttgcca ggctgtgagc
     5581 agggtatttt ccgggccgtc gaccgcaacg ccgatgtcgc ggatgcgcac cgcggcgccg
     5641 ttcttgtagg ccaccaccac gtcgttccag ggagcagctt tcaacaattg atcgttatcg
     5701 tagatcgcca tgctgcgctt cgggccgttg atcgatccct tgggcgcatc gaccgtcgcg
     5761 gccgtgatca cgccggccac atcctccatt tgcaagccga gcgcggcgag gcgggtggga
     5821 tcgatttgca cgcgtaccgc gggcttctgc tgtccgccta tcgacacctg cgcgacgccc
     5881 gggatctggg agatctgctg cgcaagaatg ttctcggtgt agtcatcgac ggtggtgacg
     5941 ggcagcacgt cggagtgcac ggcgaggatg aggatgggcg agtctgccgg attgaccttc
     6001 ttgtaagtgg gcggcgtggg cagattcttc ggcaactggc cgccggcggc gtcgatgccg
     6061 gtttgcacgt cttgcgcggc ggcgtcgatg ttgcgattca gatcgaattg caacgtaatc
     6121 tgcgtggtgc cgtagacatt cgtcgaggtc atttgcgcga ggccggagat ttccgcaaat
     6181 tgatattcca gcggggtcgc caccgaagcc gccatcgtct ccggactgcc gcccggcaat
     6241 tgcgccgtca cgaggatggt cgggaagtcg acctgcggca gcggggcgac cggcagcagc
     6301 gggtagacca ccagtccgac gaggaaaatg ccgcccatca acagcgaggt cgcgatcggg
     6361 cgccggacga atggctcgga aatactcatg atgcgttcgc ggcttgcggg ctgcgagtgg
     6421 ccgcttcggc ggttgccgcg cgcacatgca cgcccgcctg caggcgatac tggttgctgg
     6481 tgaccacgtg ctcgttcaat gcgagtccgg cgttcaccac ggtcaagccg ccgctgtcgt
     6541 cgccgatctt gatttgccgc acctccacgg tcgaatccgg cttcaccacg taggtgaaag
     6601 gaccgttggg cccgagttgt accgccgccg acggcatagt gagcgcatcg tgcagcgtgc
     6661 gcaccaacac gcgggcattg acgaactggc cgggccagag cgtgttgtgg gcattcgcga
     6721 acgtcgcctt gagcttcgcg gtgccggtcg gctgatcgat ttcgttgtcg accagcgtga
     6781 gggtgccttg atcgagttcg gtgccgccgc cgcgtgcgac ggtggtgacg ctgacgggtc
     6841 cggcggcgag cgcagtattg acggtcgcga gatcttcttc gggcagcgtg aagatcacgg
     6901 aaatcggctg cacctgagtc accacgacga tgccggtggt ggcggcggca tgcacgatgt
     6961 tgccggggtc gaccaggcgt atccccgtgc gtccgtcgat cggggaagtg atgcgcgtgt
     7021 actcgagctg cgtgcgtgcg ttgtcgatga gcgcctgatc gacttgcagc tgcgcatcga
     7081 gttgatcgac catggcacgc gcggtgtcga cggtctgctt gctcgccaag tcctgcggct
     7141 gcaggatcgt gtagcgctcg agatcgcgct tcgcgttgac gagctgggcg gcatccttgg
     7201 ccttggtggc gagtgcctgg tcaaacgccg cctgattggg gcgcggatcg atttgcgcca
     7261 acaggtcgcc cttatgcacg gtctgacctt cggtgaaggc gatcttttgc agttcaccgt
     7321 cgacgcgcgc cgtgaccgtg accgtgtaga acgcttgcac cgtgccgaga ccggagagat
     7381 aaatggggac ctcggaatgt gcgaccactg cggtgtcgac ggctatcgcc gggggcggcg
     7441 cgtgtgcagc cgcctgcgcg cggcggtaaa ccacgacgcg ccatgcaatc aacgccacca
     7501 ccgcgagcgc cagcacgccg agccatccgc agcgataacg gcgaattgtc gaagcatcgt
     7561 tcatctgtac gtcacccttc atgccaaccg ccgcccagcg cggtgaacaa gtccaccaac
     7621 gactgcaagt gcgaataatt gacttgcaac aactgatctt gtgcgctgaa cagcgcgttt
     7681 tccgtattca gaacggtcag aatattcacg gtgccggcgg acatctgctg cagtgcgaaa
     7741 tcgtaggcgc ggcgcgcaac cgtgaccgca tcttgctggc gcgtctgctg ctcggcggtc
     7801 tgttgagtcg cgaccagtgc gccctcgaca ttgctgaatg ccgagagcac cgtcttgtga
     7861 tacgtcctaa gcagttcggt atagcgggca tttgagtacg ccacctggcc gcgcagcgcg
     7921 ccgccatgaa agattggctg ggtaagacct gcggtgagcg cgtaaatgcg gttcggcgga
     7981 ctgatgaggg ccgcgagcgc cgagctctcg taaccgccgc ccgcggtcag ttcgatggag
     8041 gggaacaagg cggccctggc aacggtgatg tccgcgttgg cggcaatcag ttgctgttcg
     8101 gcttcggcca catcgggtcg gcgcgtcagg agttgcgagg gcaggccttc gacgatcacc
     8161 ggagccgaca gctcctgcag ggtcccggtg tcgacgtcca ccgactcggg ggtcttgccg
     8221 atgagcacgg cgagtgcata caccgtctgg cgcaattgct gctgcagcgg cggcagggcg
     8281 gcgctcaaca gggccacggc ggtctcctgc tgcgcgacgt cgagtccggt cgcggtaccg
     8341 gccgtttgtt ccagctggaa tcccttgaga atcttgcggc cgttgtcgag gttttgctgc
     8401 gccacccgga tgcgatcgcg caactcgagc gcttgaaaat aggtggtcgc gacgctcgag
     8461 atcacggtga gcgcgatcgt ttgctgatcg taacggctgg cgatcgcggc ggcccgcgcg
     8521 gcgtcgcgca gcgctcgatt cttgccccag aaatcgagct cgtagctggc gttaagctgc
     8581 ggattgaaat cgttgaaaac tcgcgggccg atgcccgtga cctgcgcgcg ctcgcgcgtc
     8641 gcggtggcgc ccaaatcgag cgacggcagc agcggcgcgc cggcaatacg cagctgcgcg
     8701 tcggcttcct gaacgcgtgc gatggctgcg gcgaggtcgt cgttgctgcg ctcggcttcg
     8761 tcgatcaatg cgtcgagacg cgccgagccg aacccatgcc accagtctgc ggcaggccaa
     8821 accccggccg ccgcgtcagg gcggccggca tcggccgcgg cggattcgtg ccattgcgcc
     8881 ggcgggggaa tattcggtcg ttgataggta ggtccgacgc tgcaagcgcc gagccacgcg
     8941 acaccgcaga cacaaagcgc cgcacccagg gcatgccatc tcatggtcgc taccctgggc
     9001 gcgcggcaat taagcgccta tggtcgcatt attaagatct gttaattagc taatgcgcat
     9061 cggcggcgaa gcgcgcttac tttcaatttg aaagtaagcg gcaatctgcg ctatacatac
     9121 caaatggtaa ccacatctgc cgccgagtca ttcgatttct gcaccttgtt gacttccacc
     9181 caggatcaaa tggcgcgtgc gctgctcgag aggatcaccg gcaaatggtc gctcgcggtg
     9241 atgcgcgcct tggccgaagc gggcggcccg ttgcgcttct cgagggtcct cgagcgcgtc
     9301 tcgggcatca gtcaaaaggt gctgactcag accttgcgca cgctcgaacg agacggactg
     9361 atcagccgca cgttgtatcc ccaggtgccg ccgcgagtgg aatatgaact
//
LOCUS       DASWZT010000556        10975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_82023, whole genome shotgun sequence.
ACCESSION   DASWZT010000556 DASWZT010000000
VERSION     DASWZT010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10975
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82023"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /gene="trmB"
                     /locus_tag="VGE92_15950"
     CDS             complement(<1..517)
                     /gene="trmB"
                     /locus_tag="VGE92_15950"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEY1315376.1"
                     /translation="MRSPRHIRSFVLRAGRLTAAQERALKDHWPAYGIELGEEPLDLE
                     RIFARRAPRCLEIGFGAGEVIGKLAENNPDIDYLGIEVHRPGVGRLLLRAASANVSNL
                     RVICHDAVEVLRDKVSDACFDEILIFFPDPWHKKRHHKRRLIDAAFVSCLAAKLRAGG
                     TLRLATDWQDYA"
     gene            534..2045
                     /locus_tag="VGE92_15955"
     CDS             534..2045
                     /locus_tag="VGE92_15955"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEY1315377.1"
                     /translation="MRKSYGIASAVCLLMLAGCVGLPRHVEKSRSEALSNPETTSLGR
                     LVASEQSNKNLSGIRLLASGEEALADLIALADHAERTLDIQYYIIQQDDSARLLLRHV
                     RQAADRGVRVRVLVDDLNTAGEDRCFMHLSEHANVEVRVFNPFPGGRFATWTRFLASA
                     SDMRRINHRMHNKLFVVDDALAITGGRNIGDQYFTRDEHNNFIDLDVVAAGAIVPELS
                     ASFDAFWNSKYAYPIASLASPVSAEPASAPPAESGPVNANWLEGEIDSGKLQLTWVPA
                     TVLADRPAKIASDTSPDEEVTIANNISALMRSATQELIVISPYFIPGEDGVALFSKLV
                     ENGVHIRILTNSLASTDSPLVHDGYARYRVALLKLGIELSEVRPKLGQKRARFHPFRS
                     SNASLHAKAVVIDQKTVFIGSMNMDGRSARFNSELGLVIRSAEIARQVTSLLDDISAD
                     GSYKLQLTGHGDQIEWSSGEPGAETTWFTDPETTRLQRFTLKAVAPFAPEELL"
     gene            complement(2046..2828)
                     /locus_tag="VGE92_15960"
     CDS             complement(2046..2828)
                     /locus_tag="VGE92_15960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY1315378.1"
                     /translation="MMDVIIVDDEQAGRRTLREYCEAEGDLRVVGEFGDGSAALAAIH
                     VQQPQLLFLDIQMDPLNGIDLARALQPAHLPSLVFVTAYDTYAVEAFELSAVDYLLKP
                     FDQERFRKTLERVRPRHAKSGADERQALLAGLLAQLERSARGSSEERPRLLAEFGGHL
                     HVLEAAQVEMIEADRNYVVIRVGKDSFHARSTLQQAELALRSQPMLRISRSCLVNMNY
                     VKEVNRTLRGDFILVLAGGATVTSSEGFRGKVKEYLSTLRLS"
     gene            complement(2825..4006)
                     /locus_tag="VGE92_15965"
     CDS             complement(2825..4006)
                     /locus_tag="VGE92_15965"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY1315379.1"
                     /translation="MMGIGTADASARRFAGGLSLRQFLGIACLFWVYVTLSNILYAYS
                     MRTGISRMTNVSLFAAWDARLLQHLMLLPILLVSFWASLRIQWRPLLVALPLQAILGT
                     LFSAIAYPALLASQLALGSAEWHNSGMEHVSNPWTDSFFLSLWLASFVSFLPTYGFGL
                     ALITGLALYTRFRNSELRRAALEREWSSARLAALRMQLSPHTLFNLLHTIRGHIEWDP
                     KGAQNMVVQLADLLRRLLNAGERDFSRLADELQFVRLYLELQQRRFADRLSVELPTTA
                     EIPAVWVPSLILQPLVENAVVHGLSGHQGPVTVRISAQLLEDVLTLSVFNTIAADKLV
                     GEEGIGINNVRERLAVQFEGRANLAASGADGQWCSEITMPAIHHSPVREARPNPVLVD
                     A"
     gene            4172..4534
                     /locus_tag="VGE92_15970"
     CDS             4172..4534
                     /locus_tag="VGE92_15970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HEY1315380.1"
                     /translation="MSHFTTPKTTGSRAKLALLLVGSVAGLAAAGAAGAASPSNDAPS
                     LVVKYDRQTLATDDGVNTLYRRITYAARQVCPDADIRDLAAQQHVQQCRSHAVAAAIK
                     AIDNSQLAALYAVHSKNG"
     gene            complement(4550..6871)
                     /locus_tag="VGE92_15975"
     CDS             complement(4550..6871)
                     /locus_tag="VGE92_15975"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEY1315381.1"
                     /translation="MRVHGNRTLALAFAGIALLLAATLVAYRTPAPLGLDAAPGVFSA
                     GRARSILAALVGSGVPHPIGSPANLALRETLVGRLRAMGYATQLQTGLSCNDGVCGIP
                     INIIASLGPIPKDRDAVLLAAHYDSVPAGPGASDDGAAVASLLEIARILAARPAPRHP
                     VILLLTDGEEAGLLGALLFVREHPLARAVKAAVNMEARGTSGPSLMFETGSANSWLMR
                     MYDSVIARPITNSLYYVVYKLLPNDTDFTVFKAAAYQGFNFAFIGNVGRYHTPLDNVA
                     NASAGSIQHQGDNALASLLALANSASLTAPASDAVFFDVFGRTLCIWPAAFAAAAAVV
                     CLLLLLGQVFLWRRAGVVATREILWGGLGSLCMLGVGVALSSGLLALYLALGRIPPLD
                     AESWNAQPLPMHCAAAAIAFLVAGATGHWLARRAGFWGFWAAAALLIALLSAVSAVLI
                     PGASFVWLLTAIASALAALPCTISAAQRRSPPPWAPALAALLPTLVFFAAVIPVLRFL
                     YAALGSLGWPVNTVVLCLGTATLLPLLAAATRQMRRAVIASAALTTIGGALLTLALPT
                     YSADWPQRINLEYWLDADSGQAHYLARCDSRRLPATLAAAAHFDPAPHPRFAGSAAPA
                     FHADAPATPLAAPELTLTGAPTAAVPGVTHFELRLRSARGAPEALVVFPAGAQVDAVE
                     FATAAAPLQAKLGKLKAGATLLDLVGLPAEGVQFSIDAAGSLPLVVQVFDQSYAFAHS
                     NLLRARAANATSSQDGDLTVVHRTVSLDPAADR"
     gene            complement(6895..7443)
                     /gene="ppa"
                     /locus_tag="VGE92_15980"
     CDS             complement(6895..7443)
                     /gene="ppa"
                     /locus_tag="VGE92_15980"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005225406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEY1315382.1"
                     /translation="MSIHDVNAGKNVPDEFNVIVEIPSAADPVKYEVDKQSGALFVDR
                     FMMTAMHYPANYGYVPQTIADDGDPVDVLVHTPFPLMPGVVVRCRAVGVLRMDDESGG
                     DAKLLAVPADDICPLFEHWKSIDDVPEIRLRQIQHFFEHYKDLEAGKWVKIIGWAGLD
                     VAHAEILDGIKRHRAATSAAKA"
     gene            complement(7529..8911)
                     /locus_tag="VGE92_15985"
     CDS             complement(7529..8911)
                     /locus_tag="VGE92_15985"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEY1315383.1"
                     /translation="MTGNVFGRPLSQLHSKINSWEFFSVKPLSWMTVAAGLALASCNI
                     GAKYSVGGTLTGLLGQGLVLEDNSGNNLSLSSNGTFVFSDGVKNGDAYSVTVKTQPSD
                     PSQTCSVQNGSGTIDKADVTNVIVTCAQSGRFAFVANQLSNTLSAYTINSSTGYLTAV
                     NGSPFASTGTGPVSLVVDPNGAFLYVANNGSNTVSVYSITSGALASVGVPIATGSGPL
                     ALTIDPTDSFLYVANLASNTVSAYTIDSSTGALTALGSFAVGSEPSSLKIDPSGNYLY
                     VANFGDGTVDVFAINTATGALSVVSGSPFTAGAGPVSVTIDPTGAFAYVANETADSIS
                     EYSIDTSGGLTQVSGSPLATGSSPESLVVDPAARYLYAANVTAKNQVAAYSITPSSGA
                     LTLSVSGVAAGVFPVSLALDPSGAFAYIANDTSNTVSVYAVDATSGSLTPVTGSPFAA
                     GDGARAIAID"
     gene            9005..10045
                     /locus_tag="VGE92_15990"
     CDS             9005..10045
                     /locus_tag="VGE92_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008983530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY1315384.1"
                     /translation="MRYRLLGQTGVYVSELCLGTMTYGGSKGIWENIGSLQQDAVNEQ
                     LRFAVEAGINFIDTANVYSQGKSETLLGQSLRTLGLARDQLIIATKATGTMDETPNGR
                     GQSRHHLFNQVEASLKRLQLDYIDLYQLHGFDPLTPFEESLSALNDLVRSGKVRYIGL
                     CNMSAWQIMKSLGVSERHGYSRFVSVQAYYTIAGRDLEREVVPLLQDQKLGLMVWSPL
                     AGGLLSGKYTAADDKGPSGARRTTFDFPPVDKPRGFKCVDAMRPLAEQRQVSVAQIAL
                     AWILSKQFVSSVIIGAKSMDQLRDNVAATRVQLTAEESKMLDEVSQLPQEYPGWMIAL
                     QGQYRAKAPYKE"
     gene            10148..10624
                     /locus_tag="VGE92_15995"
     CDS             10148..10624
                     /locus_tag="VGE92_15995"
                     /inference="COORDINATES: protein motif:HMM:NF022458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2844 domain-containing protein"
                     /protein_id="HEY1315385.1"
                     /translation="MPTLRSTGIVKKCVLAAGLLTAGLGPCIAVAALGEPEGSVKSDV
                     AQMRGSIKVTANANYRSHEIQTPAGTVVREFSGANGNVFAILWSGPVMPNLRQTLGQY
                     FDDYVAAAKANRAGHHHLDIHRTDLVVHASGHMRAFSGIAYLPQAVPSGVSVGELQ"
     gene            10621..>10975
                     /locus_tag="VGE92_16000"
     CDS             10621..>10975
                     /locus_tag="VGE92_16000"
                     /inference="COORDINATES: protein motif:HMM:NF023352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3443 family protein"
                     /protein_id="HEY1315386.1"
                     /translation="MIAGQGLGVAARALRMGLCAMLCLAVLSCGGGGGNSLAGGGSSG
                     STGSGTTTAVNNVVNLVVDAGPSAPSSAVVNTLYTTVKVCVPGSTTSCATIDHIEVDT
                     GSFGLRLISSALGSVS"
BASE COUNT         2199 a         3417 c         3450 g         1909 t
ORIGIN      
        1 gcgcatagtc ctgccagtcc gtggcgagcc gcagtgtgcc ccccgcgcgc agtttcgctg
       61 caaggcagga tacgaacgcg gcatcgatca agcggcgttt gtggtggcgc tttttatgcc
      121 atggatccgg gaagaaaatc agaatctcat cgaagcaggc atcgctaact ttgtccctca
      181 ggacctcgac cgcatcgtgg cagatgacgc gcaggttgct cacgttcgcg gatgccgcgc
      241 gcagcagcag ccggccgact cccggccgat gcacttcgat gcccaggtag tcgatgtcag
      301 gattattctc cgccagcttg ccgatcacct ctccggcgcc gaagccgatc tcgaggcagc
      361 ggggcgcgcg gcgcgcaaag atccgctcca aatccagggg ctcttccccc aattcgatgc
      421 cgtaggcggg ccaatgatcc ttcagggcgc gctcctgcgc cgcggtcagg cggcccgcgc
      481 gcaacacgaa acttcgaata tgacggggtg atcgcatatg ggatgatgcc ttgatgcgta
      541 aatcctacgg cattgcgagc gcggtatgcc tgctcatgct ggccgggtgc gtcggattgc
      601 cgcggcatgt ggaaaaatcc cgcagcgagg cgctgtcgaa tcccgaaacc acatcgctcg
      661 gccgcctggt cgcatcggag cagagcaata agaacctgtc cggcatccgc ctgctggcat
      721 ccggagaaga ggccttggcc gatttgatcg cgctggccga ccacgccgaa cgtacgctcg
      781 acatccaata ctacataatt caacaggacg actcggcgcg gctgctgctg cgtcatgtgc
      841 gccaggcggc ggaccgcggc gtgcgtgtgc gggtgctggt cgatgatctc aacacggcgg
      901 gtgaagaccg gtgtttcatg catttgagcg agcatgcaaa cgtcgaagtc cgggtgttca
      961 atccttttcc cggcggccgg tttgcgacct ggacgcgctt tctggcctcg gcaagcgaca
     1021 tgcggcgtat caatcatcgc atgcacaata agttgttcgt cgtcgacgat gcgctcgcca
     1081 tcaccggcgg gcgcaatatc ggcgatcaat acttcacgcg cgatgagcac aataacttca
     1141 tcgatctgga tgtggtggcg gcaggtgcca tcgtgcccga gctgtccgcg tccttcgatg
     1201 ccttttggaa cagcaagtac gcctatccga tcgcctcgct ggcttccccg gtgagcgccg
     1261 agccggcatc ggcgccgccg gccgagagcg ggccagtcaa cgccaactgg ctcgagggcg
     1321 aaatcgattc ggggaaactt cagctgacgt gggtgccggc cactgtgctc gcggatcggc
     1381 ccgccaagat cgcgagcgac acctctccgg atgaggaagt caccatcgca aataatatct
     1441 ccgcactgat gcgctcggct acccaagagc tcatcgtgat ctcgccgtac ttcatacccg
     1501 gcgaggatgg cgtcgcactg ttctccaaac tggtcgagaa cggcgtgcat atccgcatcc
     1561 tcaccaactc gttggcatcg accgactcgc cgctggtgca cgatggttac gcgcgctatc
     1621 gcgtcgcgtt gctgaagctc ggcatcgagc tctccgaggt tcggcccaag ctcgggcaga
     1681 agcgcgcacg ctttcacccg tttcgcagtt ccaacgcgag cctgcacgcc aaggcggtgg
     1741 tgatcgatca gaaaaccgtg ttcatcggtt cgatgaacat ggatggacgc tcggcgcgtt
     1801 tcaacagcga gctgggtctc gtgattcgca gcgcggagat cgcccgccag gtcaccagcc
     1861 tgctcgatga catcagcgcc gacggcagct acaagctgca gttgaccggt catggcgatc
     1921 aaatcgaatg gtcgagcggc gagccgggtg ccgaaacaac gtggttcacg gatcccgaaa
     1981 ccacgcgtct gcagcgcttt accttgaagg cggtggcgcc gttcgcgccc gaggaactgc
     2041 tgtaatcagg aaagccgcag cgtgctcaag tattccttga ccttgccgcg aaagccctcg
     2101 ctgctggtga ccgtcgcgcc gcccgccaat accaggatga aatcgccgcg cagcgtgcga
     2161 ttcacttctt tgacgtagtt catgttcacg agacaggagc ggctgatgcg cagcatcggt
     2221 tgcgatcgca acgcaagttc cgcttgctgc aaggtgctgc gcgcgtggaa actgtctttg
     2281 ccgacgcgga tgaccacata gttgcgatcg gcttcgatca tttccacttg agcggcctcg
     2341 agcacgtgca gatggccgcc gaattccgcg agcaagcgcg gccgttcctc gctcgaaccg
     2401 cgcgccgagc gttccagctg cgcgagcaat ccggcgagca gcgcctgtcg ctcatcggct
     2461 cccgacttgg catgccgcgg ccggacacgt tccaacgtct tgcggaagcg ttcttgatcg
     2521 aacggtttca ggaggtagtc caccgcgctc agctcgaagg cttcgacggc gtaggtgtcg
     2581 taggcggtga cgaataccaa ggatggcaaa tgcgccggct gcaacgcgcg cgcaaggtcg
     2641 atgccgttca gcggatccat ttgaatgtcc aggaacaaga gttgcggctg ctgcacgtga
     2701 atcgccgcga gcgcggcact gccgtcgccg aattcgccca ccacgcgcag atcgccttcc
     2761 gcttcacaat actcgcgcag cgtacggcgg ccggcttgtt cgtcatcgac gatgatgacg
     2821 tccatcatgc gtcgaccaac accggattcg gtcgcgcctc gcgcaccggc gaatggtgga
     2881 tcgcgggcat tgtaatctcg ctgcaccact gcccgtcggc gccgctcgca gcaagattcg
     2941 cgcgcccttc gaactgcacc gcgaggcgct cacggacatt gttgatcccg atgccttcct
     3001 cgccgaccaa cttgtccgcc gcaatcgtat tgaacaccga caatgtcagc acgtcctcca
     3061 gcagctgcgc gctgatgcgc acggtcaccg gaccctggtg gcccgacaag ccgtgtacga
     3121 ccgcattttc gaccaggggt tgcagaatca ggctcggcac ccacaccgcg ggaatctcgg
     3181 ccgtggtcgg cagctcgacg ctcaagcgat cggcgaagcg ccgctgctgc agctccaggt
     3241 acagtctgac gaattgcagt tcatcggcga gcctcgagaa gtcgcgctcg cccgcgttca
     3301 acagccgccg cagcaaatcc gcgagttgca ccaccatgtt ctgcgcaccc ttgggatccc
     3361 attcgatatg gccgcgaatc gtgtgcaaca ggttgaagag cgtatgcggc gacagttgca
     3421 tgcgcagcgc cgccaagcgg gccgaactcc actctcgctc gagcgccgcg cgccgcagtt
     3481 cggaattgcg aaaccgggtg tagagcgcga ggccggtgat cagcgccaac ccgaagccat
     3541 aggtcggtag aaaagagacg aagctcgcca gccacagcga taaaaagaag gagtccgtcc
     3601 acggattgct gacgtgctcc atgccgctgt tgtgccactc ggcgctgccc aatgccaatt
     3661 gcgacgccag caaggccgga taggcaatgg ccgaaaatag ggtgcccaag atcgcctgca
     3721 atggcagggc gacgaggagc ggacgccact gaatgcgcag ggacgcccaa aacgacacca
     3781 gcaggatcgg caacagcatc agatgctgca acaggcgcgc atcccaagcg gcgaacagcg
     3841 agacgttcgt catgcgcgaa atgccggtac gcatgctgta ggcgtacagg atattcgaca
     3901 aggtgacata gacccagaag agacacgcga tgccgaggaa ttgccgcagg ctcaagcccc
     3961 cggcaaaacg ccgggcggaa gcgtccgcgg ttccgattcc catcatgtgg caagggtagc
     4021 gcaactcgcc ggaattgcgc accccgctta gggtaggtcc gggacgaaac cgtgaatggc
     4081 cgggacgacc tcgacgccga cccgcagacg cgctgcgccc tcggcgtgtc ttatagacgc
     4141 gtcatcaaac agcctctgaa ggagatatgt catgagtcat ttcaccaccc cgaaaaccac
     4201 cggatcccgc gccaagcttg ccctgctgct cgtgggaagc gtggcgggcc tcgccgccgc
     4261 cggcgcagcc ggtgccgcca gcccgagcaa cgatgcgccg agcctggtcg tcaagtacga
     4321 ccggcaaacc ctagcgaccg acgacggcgt caacactctg tatcgtcgaa tcacgtacgc
     4381 cgctcgacag gtgtgcccag acgccgatat tcgcgatctt gccgctcagc agcatgtgca
     4441 gcagtgccgc agtcatgccg tggcggcagc cattaaggcg atcgacaatt cgcagctcgc
     4501 ggctttgtat gcagtgcatt cgaagaacgg ctaagtgagc tttttcgact catcgatcag
     4561 ccgcaggatc gagcgatacg gtgcgatgca cgaccgtcag atcgccatcc tgcgagctgg
     4621 tcgcgttggc ggcccgagcg cgcaacagat tgctgtgcgc aaatgcgtaa ctctgatcga
     4681 atacttgaac caccagcggt aacgagcctg ccgcgtcaat actgaattgc acgccttccg
     4741 ccggcagccc caccaaatcg agcagggtcg cgccggcctt gagcttgccg agtttggcct
     4801 gcagcggtgc cgccgcggtg gcaaattcaa cggcgtcaac ttgcgcgccc gccggaaaca
     4861 ccaccaacgc ctccggcgcg ccgcgcgccg aacgcagtcg taactcaaag tgagtgaccc
     4921 cgggcaccgc cgctgttggc gcgccggtca gcgtcaactc cggagcggca agcggggtcg
     4981 ccggcgcatc ggcatggaac gccggcgccg cgcttcccgc gaaacgcgga tgcggcgcag
     5041 ggtcgaagtg tgccgcggcg gcgagcgttg cgggcagccg gcgagaatcg cagcgcgcaa
     5101 gatagtgtgc ttggcccgag tcggcatcca gccaatactc gagattgatc cgttgcggcc
     5161 agtcggcgga gtaggtcggc aaagcgagcg tgagcagcgc gccgccaatc gtcgtgagcg
     5221 cggcgctcgc gatcaccgcg cggcgcattt ggcgcgtcgc cgctgccagt aggggcagta
     5281 gggtcgcagt ccccagacaa agcacgacgg tattgacggg ccagcccaaa ctgccgagcg
     5341 ccgcgtacaa gaaccgcaac accgggatga ccgccgcaaa gaaaaccagc gttggcagca
     5401 gcgcggcgag ggcgggcgcc cacggcggcg gtgagcggcg ctgggcggcg ctgatggtgc
     5461 agggcaacgc cgccagggcg ctcgcgatcg cggtcaggag ccagacgaaa ctggcgcccg
     5521 gaatcaacac cgcactcacc gcggacagga gagcgatgag cagcgccgcc gctgcccaga
     5581 atccccagaa gccggcgcgt cgtgcgagcc agtggccggt cgcgcccgcg accaggaagg
     5641 cgatcgccgc ggccgcacag tgcatcggca acggctgcgc gttccaagat tcggcgtcga
     5701 gaggcggtat ccgccccagc gcgaggtaga gcgccagcaa tccgctcgag agagcgacac
     5761 cgaccccaag catgcacaga gagcctaagc ccccccacaa gatctcgcgt gtggcaacca
     5821 cgcccgctcg ccgccagagg aatacctgtc cgagcagcag cagcaagcac accactgccg
     5881 ccgcggccgc gaacgccgcg ggccagatgc acagggtgcg cccaaataca tcgaagaaca
     5941 ccgcgtcgga tgcgggcgct gtcaaactcg cagaattcgc cagcgcgagg aggctcgcaa
     6001 gcgcattgtc cccttgatgt tggatgctgc ctgcgctcgc gttggcgacg ttgtcgagcg
     6061 gtgtgtgata gcggccgaca ttgccgatga aggcaaagtt gaaaccttgg taggccgccg
     6121 ctttgaagac cgtgaaatcc gtgtcgttcg gcagcagttt ataaacgacg tagtagagcg
     6181 agttggtgat cggccgcgcg atgaccgaat cgtacatacg catgagccag ctgttggcgc
     6241 tgccggtttc gaacatcaga ctggggcccg aggtgccgcg cgcctccata ttgacggctg
     6301 ccttgacggc gcgcgccagc gggtgctcgc gcacgaacag cagcgcgccc agcagcccgg
     6361 cttcttcccc atcggtgagc agcagaatga cgggatggcg cggcgccggc cgcgcggcga
     6421 gaatgcgcgc gatctcgagc aggctcgcga ccgcggcgcc gtcatccgag gctccggggc
     6481 ccgccggcac cgaatcgtag tgggcggcca gcaacaccgc atcccggtct ttggggatcg
     6541 gcccgagcga ggcgatgatg ttgatcggga tgccgcacac cccgtcattg cacgacaacc
     6601 cggtttgcaa ctgtgtcgcg taacccatcg cccggagacg cccgaccaga gtctcgcgca
     6661 gtgcaaggtt ggcaggactt cctatcggat gcggcacgcc gctgccgacc agcgcggcga
     6721 ggatcgaccg ggcgcgaccg gcagaaaaga caccaggcgc ggcatccagc ccgagcgggg
     6781 ccggcgtgcg gtacgccacc agcgtcgccg ccagcaacag ggcgatcccc gcaaaggcca
     6841 gcgccagtgt gcgattgccg tggaccctca tagatttttg ttacggcggc agcgctaagc
     6901 tttggcagcg cttgttgccg cccggtggcg cttgatgccg tcgaggatct ccgcgtgcgc
     6961 aacatcgagt cccgcccagc cgatgatctt gacccattta ccggcctcga gatccttgta
     7021 gtgctcgaag aaatgttgaa tctgccgcag ccggatctcc gggacatcgt cgatcgattt
     7081 ccagtgttcg aacagcggac agatatcgtc ggccggcacc gcgagcaact tggcatcgcc
     7141 gccggattca tcgtccatgc gcagtacgcc gactgcacgg caacggacca ccacccccgg
     7201 catcaacggg aatggcgtat ggaccaacac atcgaccggg tcgccgtcat cggcgatggt
     7261 ttgcggcaca tagccgtagt tggcgggata gtgcatcgcc gtcatcatga aacgatcgac
     7321 gaacagcgct ccggactgct tgtcgacttc gtacttcacg gggtccgcag cggacggtat
     7381 ctctacgatc acattgaatt cgtccgggac atttttcccg gcgttaacat catgaatact
     7441 catctgccgt tgatcctttc agccggcgcg caatctgcgc cggcgctcat actacaacgc
     7501 ccgcaggcgc cgcatcagtc gcaacggctc aatcgatggc gatcgcacgc gcaccatccc
     7561 cggccgcgaa cggcgaaccc gtgaccggtg tgagactgcc gctcgtggca tcgacggcat
     7621 agacagaaac agtattggac gtgtcattgg cgatgtaggc gaatgcgccc gacggatcga
     7681 gagccaaact caccggaaag acgcccgcgg caacgccgga aaccgacagg gtcaacgcgc
     7741 cgctgctcgg cgttatggaa tatgcggcta cttggttttt cgcggtaaca tttgcggcat
     7801 atagatagcg ggctgccggg tcgaccacga gggattccgg gctgcttccc gtggccagcg
     7861 gcgagccgga tacttgcgtc aggccgccgg aggtatcgat cgaatattcg gaaatggaat
     7921 cggccgtttc gttggcgacg taggcgaaag ctcccgtggg atcgatggtc accgatacgg
     7981 ggccggcgcc ggcggtgaac ggcgatccgg agactacact caaggcaccg gtggcggtat
     8041 tgatcgcgaa tacatcgaca gtaccgtcgc caaaattagc gacgtagagg taattgccgc
     8101 tcggatcgat cttgagggaa ctcggctcgc tgcccaccgc gaaactgccg agcgccgtga
     8161 gcgcgccggt ggaactgtcg atggtgtagg ccgacacggt gttcgacgcg agattcgcga
     8221 catacagaaa gctgtcggtg ggatcgatcg tcaacgccag cggtccgctg ccggtggcga
     8281 tcggcacgcc gacgctcgct agcgctccgc tggtgatcga atacacggaa acggtgttcg
     8341 atccgttgtt ggcgacgtag agaaaggcgc cgttcggatc caccaccaag gacacgggac
     8401 ccgtgcccgt cgaggcgaag ggtgagccgt tgaccgccgt caagtagcct gtcgaggaat
     8461 tgatggtata cgcggacagc gtattcgaca gctgattcgc gacgaaggcg aagcgcccgc
     8521 tctgtgcgca agtgacgatc acattggtga catcggcctt atcaatggtc ccggagccat
     8581 tttgcacgga gcaggtctgc gacggatccg acggttgcgt tttcacggtc accgagtaag
     8641 cgtcgccgtt tttgacgccg tccgagaaga cgaatgtgcc gttggacgac aggctcaaat
     8701 tgttgcccga attgtcctcg agcaccaagc cttggccgag gaggccggtc agcgtgccgc
     8761 cgaccgagta cttcgcgccg atattgcagc tcgcaagcgc gagcccggcg gcgacggtca
     8821 tccaggaaag tggcttcacg gaaaaaaact cccaggaatt gatcttgctg tgcagctgag
     8881 acagcgggcg gccgaaaaca ttcccggtca tgccgctgtt ccgcgtcaag aactgtgtag
     8941 gtgaatcatt gtaagcttgg ctgaccccgt tcaacaaact cactggcagt aactggagat
     9001 catcgtgcgc tatcgcttac tcggccaaac cggcgtgtat gtatccgaat tgtgcctggg
     9061 gaccatgacc tatggcggca gcaagggaat ttgggagaat atcggcagcc tgcagcagga
     9121 tgcggtaaac gagcagctgc gtttcgcggt cgaggcgggc attaatttca tcgataccgc
     9181 caacgtctac tcccagggga aatccgaaac gctgttgggt cagtcgttgc ggacattggg
     9241 attggcgcgc gatcagctga ttatcgcaac caaggccacg ggcacgatgg acgaaacacc
     9301 gaacggacgc ggccaatcgc ggcaccactt gttcaaccag gtggaagcca gcttgaagcg
     9361 gctgcagctc gattacatcg atctttatca attgcacggc ttcgatccgt tgaccccctt
     9421 cgaggagtcg ctgtctgctc tcaacgactt ggtgcgctcc ggcaaggtgc gctacatcgg
     9481 cctgtgcaac atgagcgcct ggcagatcat gaaatccctg ggtgtgtcgg aacgacacgg
     9541 ctattcgcgg ttcgtcagcg tgcaggccta ctacaccatc gccggccgcg atctcgagcg
     9601 tgaggtcgtg ccgctgctgc aggatcagaa gctcggcctg atggtttgga gcccgctcgc
     9661 gggcggcctt ctcagcggca agtacacggc cgccgatgac aagggaccga gcggcgcgcg
     9721 gcgcaccacg ttcgattttc cgccggtaga caagcctcgc ggcttcaagt gcgtcgatgc
     9781 catgcgtccg ctcgcggagc agcgccaggt ttccgtggcg caaatcgcgc tcgcttggat
     9841 tttgagcaag caattcgtca gcagcgtgat catcggcgca aaatccatgg atcaattgcg
     9901 cgacaacgtc gcagcgacac gcgtgcaact caccgccgaa gaaagcaaaa tgctcgatga
     9961 agtcagtcaa ttgccccagg aatatcctgg ctggatgatc gcgctgcagg ggcagtatcg
    10021 agccaaggcg ccttacaagg agtaacctcg ctgtctgcaa aataagacat gccgattgtg
    10081 atctccgacc ttcatcgagt gaaccaaaaa gcggtaatgt gactccaagc cagcaaagga
    10141 tgaccctatg ccaaccctgc gatcgaccgg aatcgtcaag aaatgtgtgc tcgccgcagg
    10201 actgctgacc gcgggtttgg gtccgtgcat cgccgttgcc gccttgggtg agcccgaggg
    10261 ctcggtgaaa tccgacgtgg cacagatgcg aggctcaatc aaagtgaccg cgaacgcgaa
    10321 ctatcggtcg cacgaaatcc agacgcctgc cggaaccgtg gtgcgcgagt tcagcggagc
    10381 gaacggcaac gtgtttgcga tcctgtggag cggcccggtg atgcccaatc tgcgccagac
    10441 cttgggacaa tacttcgacg attacgtggc ggcggcgaaa gccaaccgcg cgggacatca
    10501 ccatctcgac atacatcgaa ccgatctggt cgtgcacgct tcgggtcaca tgcgcgcgtt
    10561 ttcgggaatc gcctatctcc cacaggcagt accgagcggt gtgagcgtag gagagttgca
    10621 gtgattgccg ggcaagggtt gggcgttgcg gcgcgtgcct tgaggatggg gctgtgcgcc
    10681 atgctgtgcc ttgcggtgct gtcctgcggc ggcggtggcg gtaactcttt ggccggcggc
    10741 gggtccagcg ggtccaccgg ttccggaacc acgacggccg tcaacaacgt ggtgaacctc
    10801 gtggtcgatg cgggaccttc ggcgcccagc agcgcggtcg tgaacacgct ttacaccacg
    10861 gtgaaggtgt gcgtccccgg cagtaccacg agttgcgcaa cgatcgatca cattgaagtc
    10921 gataccggct cgttcggttt gcgcctgatc tcatcggcgc tcggcagtgt cagct
//
LOCUS       DASWZT010000557         2942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_62533, whole genome shotgun sequence.
ACCESSION   DASWZT010000557 DASWZT010000000
VERSION     DASWZT010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62533"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VGE92_16005"
     CDS             complement(<1..288)
                     /locus_tag="VGE92_16005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315387.1"
                     /translation="MPSRIPEGETRGWIVPIGGAENKENDRHILERFVRVSGGADADI
                     VVIPTASRMHETGPRYEKLFKDLGAAQVTVMDFDTRRDCQEAGRLRRLEEAS"
     gene            513..1424
                     /locus_tag="VGE92_16010"
     CDS             513..1424
                     /locus_tag="VGE92_16010"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEY1315388.1"
                     /translation="MAGSQDFAADPRNATALVSLNGQLMARNLAKVSIFDGGFVVGDG
                     VWEGLRLHRGTLLFLDQHLDRLYWGAEKIRLDIGLARPALIAEVQRLLSANQMRDGVH
                     IRLMVTRGEKSAPNQDPRNAVGPATIVIVAEYKVPDPKLATRGLSLHTASIRCTPAHM
                     FDMRLNSHSRLNLITALLEAIDAGADEALMLDPQGFVSSCNATNFFCVRDGEVLTSSG
                     ECCFNGVTRANVIHLCERHDLRLRLGNFTLDEARGAQEAFVTGTFGGLTPVREIDGRP
                     LVDPLPGPITSRLRAFYESLKDAQVPP"
     gene            1424..2173
                     /locus_tag="VGE92_16015"
     CDS             1424..2173
                     /locus_tag="VGE92_16015"
                     /inference="COORDINATES: protein motif:HMM:NF040370.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315389.1"
                     /translation="MQAHPIRIAMWSGPRNISTAMMRSFEARGDAAVSDEPFYAAYLE
                     RTGLDHPMREAVIASQPRDWRDVAAMLVGPVPGAKPIWYQKHMTLHMLDGFGRGWMAQ
                     VRNAFLIREPAAVLASYVLKRESATLADIGFVQQRALFEAEADRLGRAPPVADAADIL
                     AAPAQTLTRLCEALGIPFTPAMLHWPSGRRATDGVWAPAWYQAVEKSTGFEPPRSKTA
                     RELPTQLRKLAEQAQPHYDALARHRLAPIHA"
     gene            complement(2187..2642)
                     /locus_tag="VGE92_16020"
     CDS             complement(2187..2642)
                     /locus_tag="VGE92_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005107243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1315390.1"
                     /translation="MTQENMEIRPLSADQHAAWLPLWRGYQSFYKTDIAPEVSAVTWT
                     RLLDPAEPMGAALAWDGANAVGLVHHIRHRSCWTIGDYLYLQDLFVSPEARGGGVGRK
                     LIEYVYEVARRAGCTRVHWLTHETNSNAMLLYDRIAERSGFVQYRRAVK"
     gene            complement(2669..>2942)
                     /locus_tag="VGE92_16025"
     CDS             complement(2669..>2942)
                     /locus_tag="VGE92_16025"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY1315391.1"
                     /translation="RRYLDPALAQKLALAEFDGGTKSPVERLSEREFEVFVRLAGGAT
                     VQRIAVDLKLSASTIGTHLYNIKQKLNVANQSELTLIAIRHGLISA"
BASE COUNT          537 a          913 c          922 g          570 t
ORIGIN      
        1 gctggcctct tcgagccggc gcagccgccc ggcttcctgg caatcacggc gcgtgtcgaa
       61 atccatcacc gtgacttgcg cggcgcccag gtccttgaac agcttttcat agcgcggtcc
      121 ggtctcgtgc atgcggctgg ctgttgggat aacgacgata tcggcgtccg cgccgcccga
      181 tacccgtaca aagcgttcca ggatgtggcg atcgttctcc ttattctcgg cgccgccgat
      241 cggtacgatc caaccgcggg tctcaccctc gggaattcga ctaggcatta acggcagcgc
      301 ctcctgaagc gcaaggggtc acaggcgaat aatgacggtt tgcgcgcgat ctgtcgcgga
      361 atcacgattc tagggggtcg gcgcctcaat accaatggtt cgagcccaaa ctgcgacatg
      421 ggttgtcggt cttatgacag cgcgcgtttc gttaggcaaa ctagctgggg cggctggcgc
      481 gtgcggtttc aggagatggc ggaacttatc atatggccgg aagtcaggac ttcgcagccg
      541 atcctcgcaa tgccacggcg ctggtaagcc tgaacggtca gctcatggcg cggaacctgg
      601 ccaaggtgtc gattttcgac ggcggcttcg tggtgggcga tggggtatgg gagggtctgc
      661 ggctgcatcg aggcacgctg ctgtttctgg atcaacacct ggatcgactc tactggggtg
      721 ccgagaagat ccggctcgat atcgggcttg cccggccggc gctcatcgcc gaggtacagc
      781 gcctgctgag tgcgaaccag atgcgcgatg gcgtccacat tcgcctgatg gtgacgcgag
      841 gtgaaaagag cgcacccaat caagatccgc gcaatgcggt gggaccggcc acgatcgtga
      901 tcgtcgccga atacaaggtg cccgatccta agctcgcgac tcgcggattg agcctgcaca
      961 cggcctcgat ccgctgcact cctgcgcaca tgttcgacat gagattgaat tctcacagcc
     1021 gcctcaatct catcacggcg ctgctcgagg cgatcgacgc cggcgcggat gaagcactga
     1081 tgctcgatcc gcagggtttc gtatcgagct gcaacgcaac taatttcttc tgtgttcggg
     1141 acggcgaggt gctgacatcg agcggcgaat gttgtttcaa cggcgtcacc cgcgccaatg
     1201 tcatccatct atgcgagcgg catgacttgc gcctgcgtct cggcaacttc acgctcgacg
     1261 aggcacgtgg cgcgcaggaa gcattcgtca ccggaacatt cggcgggctg acgcccgtgc
     1321 gcgaaatcga cggccgcccc ctggttgacc cattgccggg tcccattacc tcgcgcttac
     1381 gcgcgttcta cgagtcgttg aaggatgcgc aggtgccgcc ctgatgcaag cccatccgat
     1441 ccgcatcgcc atgtggtcgg gcccgcgcaa catatcgacc gcgatgatgc gttcgttcga
     1501 ggcgcgcggc gatgccgcag tgagcgacga gccgttctac gctgcatatc tcgaacgcac
     1561 gggactcgat cacccgatgc gcgaggcggt cattgcttcg cagccgcggg attggcgcga
     1621 cgtcgccgcg atgctcgtgg gtcccgtgcc gggcgcaaag ccgatttggt atcagaagca
     1681 tatgaccctt cacatgctgg acgggttcgg tcgcggctgg atggcgcaag tgcgcaatgc
     1741 cttcctcatt cgtgagcctg ctgcggtgct cgcgtcctat gttttgaagc gcgaatccgc
     1801 caccttggcg gatatcggtt ttgtacagca acgtgcgctg ttcgaagccg aagcggaccg
     1861 cttgggacgg gcgcctccgg tggcggacgc cgccgacatc ctggccgctc ctgcgcagac
     1921 gttgacgcgg ctctgcgaag cgctcggcat cccgttcacc ccggcgatgc tgcactggcc
     1981 ctcgggacgg cgcgcgacgg acggtgtctg ggcgccggcc tggtaccaag cggtggaaaa
     2041 gtccaccggc ttcgagccac cgagatccaa aaccgcccgc gagttaccga cacagcttcg
     2101 caaattggct gaacaggcac agccgcatta cgatgcgttg gcgcggcatc ggctcgcgcc
     2161 catccatgca tagaccggca agcgagctac ttgaccgcgc gtcgatattg cacgaaaccc
     2221 gagcgttccg cgatgcggtc gtacaacaac atggcgttgg agttcgtctc gtgcgtcagc
     2281 cagtgcacgc gcgtgcagcc tgcgcggcgc gcgacttcat acacgtattc gatgagtttt
     2341 cgaccgactc cgccgccgcg cgcctcgggg ctcacgaaca agtcctgcaa atacaggtag
     2401 tcgccgatcg tccagcagga gcggtgacgg atatggtgca ccagcccaac tgcattcgca
     2461 ccgtcccagg ccaacgcggc gcccatgggt tccgccggat ccaacaaccg cgtccaagtg
     2521 acggccgaca cctcgggtgc aatgtcggtc ttgtaaaagg attggtagcc acgccacagc
     2581 ggcagccacg cggcgtgctg gtcggcggat agcgggcgaa tttccatgtt ctcttgggtc
     2641 atgcatccat ccgataacga cgtgaaggtc aggcactgat caggccgtgg cgaatcgcaa
     2701 tcagggtcag ctccgattgg ttcgcaacgt tcagtttctg tttgatattg tatagatgcg
     2761 tgccgatggt cgatgcggaa agtttcaaat ccacggcgat gcgctgcacc gtcgcgccgc
     2821 cggcgagtcg aacgaacacc tcaaattccc tctccgacag ccgctcgacc ggcgacttcg
     2881 tgccgccgtc gaattcggcc aatgccagct tctgtgcgag cgccgggtcg agatagcggc
     2941 gg
//
LOCUS       DASWZT010000558         1583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_86704, whole genome shotgun sequence.
ACCESSION   DASWZT010000558 DASWZT010000000
VERSION     DASWZT010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86704"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VGE92_16030"
     CDS             <1..222
                     /locus_tag="VGE92_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315392.1"
                     /translation="LRGDGRFNVILRNVGAAFVGTHTAAVITVTAYVIADSSQVFAPH
                     LSRGDVRAMAEKSDNGAGYDYLKKTGTLP"
     gene            complement(219..980)
                     /locus_tag="VGE92_16035"
     CDS             complement(219..980)
                     /locus_tag="VGE92_16035"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEY1315393.1"
                     /translation="MRRGAVLAVCLFVASAAVTIAGCRSMSAMGAMPMPGGWTMSMAW
                     MRMPGQTWCGAAAAFLGMWVAMMLAMMLPSLVPMLSRYHRATGATRETAFHWPTAVVG
                     AGYFSVWTAFGMAVFPVGAALAAAEMRVPGLARAVPLAVGLTIMIAGALQFTAWKAHH
                     LACCRRSPDRGVLPDAGTAWQHGVRLGLHCTSCCAGSTAILLAIGVMDLRAMLVVTAA
                     NAVERFAARGERAARALGALSVGTGLLWVARAAGL"
     gene            complement(985..>1583)
                     /locus_tag="VGE92_16040"
     CDS             complement(985..>1583)
                     /locus_tag="VGE92_16040"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HEY1315394.1"
                     /translation="AYVFDGPQGKASLLDLFGGRRQLIVYRAFFEPGVHGWPEHACIG
                     CSLGADQVSHLAHLNARDTTLVYASRAPQADIARLKARMDWEIPWYTLTDSFDADFGV
                     DEWHGHNAFIRDGERVFRTYFINQRGDEAMGSVWSYLDMTALGRQESWENSPQGYPQS
                     RPYKWWNWHDMYVPQAAPDPTWVKVSDAGEAAFRKKEG"
BASE COUNT          301 a          560 c          486 g          236 t
ORIGIN      
        1 ttgcgaggcg atggccgctt caacgtgata ctcagaaacg tcggcgccgc attcgtgggc
       61 acgcacacag ccgcagtgat cacggtcact gcctacgtga ttgccgacag ctctcaagtc
      121 tttgcgcccc acctctcccg cggggacgta cgcgccatgg cagagaaatc ggacaacggg
      181 gcgggctacg actacttgaa gaagacagga actctcccct agagcccggc cgctcgcgcg
      241 acccagagca agcccgtccc gacgcttaag gcaccgagcg cacgcgccgc gcgctcaccc
      301 cgcgctgcga atcgctcgac ggcattcgct gcggtcacga ccagcatcgc tcgcaggtcc
      361 atgactccga tggcgagaag gatcgccgtc gagccggcac agcacgaagt gcagtgcagg
      421 cccagacgca cgccatgctg ccacgccgtg ccggcgtctg gcagcacacc gcgatccggt
      481 gaccgccggc aacaggcgag gtgatgtgcc ttccacgccg tgaactgcag cgcgccggcg
      541 atcatgatcg tcaagcccac ggcgagcggc acggcgcgcg ccagcccagg cactcgcatc
      601 tcggccgccg cgagcgcagc ccccaccgga aagacggcca ttccgaacgc cgtccacacc
      661 gagaagtagc ctgcgccgac gacggcggtc ggccaatgga aggcggtttc gcgggtcgcg
      721 cctgttgccc ggtggtaacg cgacagcatg ggcaccaacg acggcagcat cattgccagc
      781 atcatggcga cccacatgcc aagaaatgcg gccgccgcgc cgcaccaagt ctgtccgggc
      841 atccgcatcc acgccatcga catcgtccag ccgccgggca tcggcatcgc acccatcgcc
      901 gacatggacc tgcatcccgc gatcgtcacg gcagcgctcg cgacgaaaag gcacactgca
      961 agaacggcgc cgcgtctcat cgaactaacc ctcttttttc cgaaaggcgg cctcaccggc
     1021 atccgatacc ttgacccacg tgggatcggg tgccgcttgc gggacataca tgtcgtgcca
     1081 gttccaccac ttgtagggtc ggctctgggg atagccctgc ggcgagttct cccatgactc
     1141 ctgccgtccg agcgcggtca tgtccaggta gctccaaacg ctgcccatcg cctcgtcgcc
     1201 gcgctggttg atgaagtagg tacggaacac gcgctcgccg tcgcggatga atgcgttgtg
     1261 gccgtgccat tcatccactc cgaaatccgc atcgaagcta tcggtgaggg tgtaccacgg
     1321 gatctcccag tccatgcgcg ccttgaggcg cgcaatatcg gcctgcggcg cacgcgaggc
     1381 atacaccagg gtcgtgtcgc gcgcgttcaa gtgggcgagg tggctcacct ggtcggcccc
     1441 gagagaacag ccgatgcagg cgtgctccgg ccagccgtgc acgcccggct cgaagaaagc
     1501 gcggtagaca atcagctgac ggcggccgcc gaacaagtcg agcaggctcg ccttgccttg
     1561 cggcccgtcg aacacgtacg cct
//
LOCUS       DASWZT010000559         4378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_43374, whole genome shotgun sequence.
ACCESSION   DASWZT010000559 DASWZT010000000
VERSION     DASWZT010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43374"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VGE92_16045"
     CDS             complement(<1..263)
                     /locus_tag="VGE92_16045"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY1315395.1"
                     /translation="MTLTAHAADARPVFWPRKTLAEELRLQLADEIVRGSLAPGAALD
                     ETTLARRFEVSRTPVREAIRLLAASGLVEVRAHRAAVVARPGA"
     gene            complement(260..1711)
                     /locus_tag="VGE92_16050"
     CDS             complement(260..1711)
                     /locus_tag="VGE92_16050"
                     /inference="COORDINATES: protein motif:HMM:NF006631.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtzE family amidohydrolase"
                     /protein_id="HEY1315396.1"
                     /translation="MPSRRRFSRPDDMDVSQPWATAAEIAAAVTSGRTSALAVTEAAL
                     ARITELNPTLNAFTAVTAQRARTRAGAIDALRGKRPPGPLAGVPFAVKNLFDIAGLAT
                     VAGSKINRAHPPAPKDSPLIERLEAAGAVLVGALNMGEYAYDFTGENIHDGPSRNPHD
                     LARMTGGSSGGSGGAVAGGLVPIALGSDTNGSIRVPSSLCGIFGLKPTYGRLTRARSF
                     PFVASLDHLGPFARSVRDLALAYDVMQGPDADDPACAQRATEPATPVLERGIEGLRIA
                     VAGGYFRQGAFPEALKAIDLVAKALGAVEEIEIPQAHRARAAAYVITATEGAALHLDR
                     LRKQARDFDPAVRDRLIAGAMVPSTLVVKAQKFRRWYRERVLELFTRCDVILAPATPC
                     TAPKIGQQNFMLGGAELPVRANMGLCTQPISFIGLPVVAVPVPLQPLPIGVQIITAPW
                     REDIALRVAYALEQMGAVAAPRPSLKPSLKVPA"
     gene            complement(1680..1868)
                     /locus_tag="VGE92_16055"
     CDS             complement(1680..1868)
                     /locus_tag="VGE92_16055"
                     /inference="COORDINATES: protein motif:HMM:NF024714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4089 domain-containing protein"
                     /protein_id="HEY1315397.1"
                     /translation="MAIPPDQLDEFIAAAARALEIPLDPEWKPAVKSNLDVTLKHAAV
                     VAEFEMPDDAEPAPIFKA"
     gene            complement(1881..3632)
                     /locus_tag="VGE92_16060"
     CDS             complement(1881..3632)
                     /locus_tag="VGE92_16060"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1315398.1"
                     /translation="MAKKTRTAAKIDRSAASTLPLLDIRDLTVEFATRRGAVRAVQHV
                     SLSVAKGETLAIVGESGSGKSVTSYAVMRILDRAGRIAQGSVMFSGLDLRGVPESEMR
                     DLRGREMSMIFQSPRLALNPIRKVGRQIEDVLLQHSQVDSDAVGERAIETLEQVRIAR
                     PRERYHAYPFELSGGMCQRVVIALALACRPQLLIADEPTTGLDVTTQKAVMDLVVELT
                     RERGMSTILITHDLGLAAAYCDRVVVMEKGLIVETASSATIFKRPSHQYTRKLMRATP
                     RPGVSLRDLLPEGETARPKAAAKTPARKTKAGGTPLLVVEKLVKEYPRQGGARSLFKI
                     FGPKPPKELETFRAVDGISFSIAHGESVGLVGESGCGKSTTSMMVMRLIDKSSGLIMF
                     DGEDIGEIPARKFATLPFRKRIQMVFQDPTDSLNPRFTAVRAIADPLLQLGGISRREV
                     RTRCEELATQVGLPLNLLDRFPHQLSGGQKARVGIARAIALKPDLVILDEPTAALDVS
                     VQAVVLNLLQDLKDSLGMSYLFVSHDLNVVRLLCDRVIVMQTGQIVEQGPTKRVMDEP
                     QADYTRDLLSAIPHPPL"
     gene            complement(3636..4367)
                     /locus_tag="VGE92_16065"
     CDS             complement(3636..4367)
                     /locus_tag="VGE92_16065"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1315399.1"
                     /translation="MPYDPLASDTTATLHAPSWHHWFGTDQLGRDIFSRVIAATRLDF
                     VIAITSVVLVFAMGGLAGISAGFFLGWTDRIVGRISDTIMAFPLFVLAMGIVAALGNT
                     VTNIVIATAIINFPLYVRVARAEANVRREAGFVQAARLSGNGEFRILLAHILPNIMPI
                     MMVQISLTMGYAILNAAGLSFIGLGVRPPTPEWGIMVAEGAANIISGEWWIALFPGAA
                     LMLAVFTFNLLGDGLRDLVDPQRRT"
BASE COUNT          813 a         1366 c         1420 g          779 t
ORIGIN      
        1 tcggcgcctg gtcgcgccac aacggcggcg cggtgcgcgc gcacttccac caatccgctt
       61 gccgccagca accggatcgc ctcgcgcact ggtgttcggg aaacctcaaa acggcgcgcc
      121 aacgttgttt cgtcgagcgc ggcgcccggc gccagcgatc cacgcacgat ctcgtcggcg
      181 agttgcaggc gtaattcctc ggcgagcgtc ttgcgtggcc agaagacggg tcgcgcgtcc
      241 gcagcgtgcg cggtcaaggt catgccggca ccttgagcga aggtttcaac gacggccggg
      301 gcgcagcgac tgcccccatc tgctccagcg cgtaggcgac ccgcaaagcg atatcctcgc
      361 gccacggtgc cgtgatgatt tgcacgccga tcggcagcgg ctgcaatggc actggcaccg
      421 cgaccaccgg cagaccgata aatgaaatcg gctgcgtgca gagacccata ttcgcccgta
      481 ccggcagttc ggcaccgccc agcatgaagt tttgctggcc gatcttcggc gccgtgcagg
      541 gtgttgccgg cgcgagaatg acatcgcagc gtgtgaacaa ttcgaggacg cgctcgcgat
      601 accagcggcg gaatttctgt gccttcacca cgagcgtgga tggcaccatg gcgccggcga
      661 tcaaccggtc gcgcacggcg gggtcaaaat cgcgcgcctg tttgcgtaga cgatcgaggt
      721 ggagtgccgc gccttcggtg gcggtaatga cataggccgc tgcccgcgcg cgatgggcct
      781 gcggaatttc gatttcctcg accgcaccaa gcgctttggc gacgaggtcg atcgccttta
      841 acgcctctgg aaaggcgccc tggcggaaat agccgccggc tacggcgatg cggaggccct
      901 caatgccgcg ttcgagcaca ggcgttgccg gttcggtcgc gcgttgtgcg caggcagggt
      961 cgtcggcatc cggtccttgc atgacgtcat aggcaagcgc gagatcgcgg accgagcgtg
     1021 cgaacggccc caggtggtcg aggcttgcca cgaacgggaa cgaacgtgcg cgcgtgagcc
     1081 ggccataggt cggcttaagg ccaaagatac cgcacagcga ggagggcacc cgtatcgacc
     1141 cattggtgtc cgacccgagt gcgatcggca ccaggccacc ggccaccgcg ccgccggagc
     1201 cgccagacga gccgccggtc attcgggcaa ggtcatgcgg attgcgcgac gggccatcgt
     1261 gaatattttc gccggtgaag tcgtaggcgt attcacccat attgagcgca ccgaccagca
     1321 ccgcaccggc tgcttctagc cgttcgatca ggggtgagtc cttcggagct ggcggatgcg
     1381 cacgattgat cttggagccc gccacggtcg cgagccccgc gatatcgaat aagttcttca
     1441 ccgcgaacgg cacgcccgca agcggtcccg gcggccgctt gccacgcaaa gcgtcgatgg
     1501 cgccggcccg cgtccgcgca cgctgggcag taacggcggt gaaggcgttg agcgtcggat
     1561 tgagctcggt aatacgggca agcgcggctt cggtgacggc aagcgcgctg gtcctgccgg
     1621 acgttaccgc tgcggcaatt tctgcggcgg tggcccatgg ttgcgacaca tccatatcgt
     1681 caggccttga aaatcggcgc cggctcggca tcgtccggca tctcgaattc cgccacgacg
     1741 gcggcgtgct tcaaggtgac gtccagattg gacttcaccg cgggcttcca ttccggatcg
     1801 agaggaattt ccagcgcacg cgctgcggcc gcgataaatt cgtccagctg atcgggtggg
     1861 atcgccattt tattcctccc ttacagcggc gggtgaggaa tcgccgacag caggtcgcgc
     1921 gtgtagtcgg cttgcggttc gtccatgacg cgcttcgtcg gcccttgttc gacgatctgt
     1981 cccgtctgca tcacgatgac acgatcgcac aggaggcgca cgacgttgag atcatgcgac
     2041 acaaaaagat agctcatccc caacgaatct ttgaggtctt gcaataggtt aagaacgacc
     2101 gcctggaccg agacatcgag cgcggcggtt ggttcgtcga gaatgacaag atcgggcttg
     2161 agcgcgatgg cgcgggcaat gccgacgcgc gctttttggc cgccggataa ctgatgcggg
     2221 aagcgatcga gcagattgag cggcaggccc acttgggtgg cgagttcttc gcagcgcgtc
     2281 cgcacctcgc gtcgcgaaat cccgccaagc tgcaagagcg gatcagcgat cgcgcgcaca
     2341 gcagtaaagc gcgggttgag gctgtcggtc ggatcctgaa acaccatttg gatgcgctta
     2401 cggaacggca gcgttgcgaa ctttcgcgcc gggatttcac cgatgtcttc gccatcgaac
     2461 ataatcaggc cggacgactt gtcgatcagc cgcatcacca tcatcgaggt ggtggatttg
     2521 ccgcagccag attcgccgac caagccgacg ctttcgccgt gagcgatcga gaaactgatg
     2581 ccgtccaccg cgcggaaggt ttcgagttct tttggcggct ttgggccgaa tattttgaac
     2641 agtgatctag cgccgccctg gcgtggatat tccttcacca gcttttcgac caccagcagt
     2701 ggcgtcccgc cggctttggt cttgcgcgcc ggcgtcttgg cggctgcctt cggccgcgcc
     2761 gtctcgccct ccggcagcag atcgcgcagc gaaacaccgg ggcgcggcgt ggcgcgcatc
     2821 agcttgcggg tatattggtg cgatggcctt ttaaagatgg tcgccgacga cgcggtctcg
     2881 acgatcaggc ccttttccat caccacgacg cggtcgcaat aggcggccgc cagtccgaga
     2941 tcgtgcgtga tcaggatcgt cgacatgccg cgctcgcgcg tgagttcgac caccagatcc
     3001 atcaccgcct tctgcgttgt gacgtcgagg ccggtggtcg gctcatcggc aatcagcaat
     3061 tgcggacggc aggccagcgc gagcgcgatc accacgcgct ggcacatgcc gccggatagc
     3121 tcgaacggat aggcatgata gcgttcgcgc ggccgcgcga tgcgaacctg ttccagcgtc
     3181 tcgatcgcgc gctcgccgac tgcgtcgctg tcgacctgcg aatgttgcag cagcacgtcc
     3241 tcgatctggc ggcctacctt gcgaatcgga ttgagcgcca accgggggct ttggaagatc
     3301 atcgacattt cgcggccgcg caggtcgcgc atttcgcttt ccggcacgcc gcgcaggtcg
     3361 aggccgctga acatgaccga gccttgggcg atgcggccgg cgcgatcgag gatacgcatc
     3421 accgcatagg acgtgaccga ttttcccgaa ccggattcac cgacaatggc aagcgtttcg
     3481 cccttggcga ccgatagact gacgtgctgg accgcgcgga cggcgccacg ccgggtggcg
     3541 aactcgaccg tgaggtcgcg gatgtccagc aatggcaagg tgctggcggc actacggtca
     3601 atttttgcgg ccgtgcgggt tttcttggcc atgattcagg tccgccgctg cgggtctacc
     3661 aggtcgcgca agccatcgcc cagcaggttg aaggtaaaca ccgccagcat tagcgcggcg
     3721 cccggaaaca atgcgatcca ccattcgccg gagatgatat tggccgcgcc ttccgccacc
     3781 atgatgcccc attccggcgt tgggggacgc acgcccagcc cgatgaacga caggccggcg
     3841 gcgttgagaa tcgcatagcc catggtgagc gagatctgca ccatcatgat cggcatgatg
     3901 ttgggcagga tatgcgccag tagaatgcgg aactcgccgt tgccggacag ccgcgccgcc
     3961 tgcacgaagc cggcctcgcg ccgcacattg gcctcggcgc gggcgacccg gacatagagc
     4021 ggaaaattga tgatcgcggt ggcgattacg atattggtca cggtgttgcc cagagccgcg
     4081 acaatgccca ttgccagcac gaacagcgga aacgccatga tggtgtcgga gatgcgccca
     4141 acaatgcggt cggtccagcc caggaaaaaa ccggcgctaa tgccggcgag cccacccatt
     4201 gcaaagacca gcaccaccga tgttattgca atgacaaaat cgaggcgcgt ggctgcgatg
     4261 acgcggctga aaatgtcgcg accgagttgg tcggtaccga accagtgatg ccatgacggt
     4321 gcatgcagcg tggccgtggt gtcgctcgcc agcggatcgt acggcacaat atacgggc
//
LOCUS       DASWZT010000560         2081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_52925, whole genome shotgun sequence.
ACCESSION   DASWZT010000560 DASWZT010000000
VERSION     DASWZT010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52925"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1881)
                     /locus_tag="VGE92_16070"
     CDS             complement(<1..1881)
                     /locus_tag="VGE92_16070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY1315400.1"
                     /translation="MRGKQPVLASDEESATLDALRYSLASEIVELVVLTSDEIFLQTL
                     REAVGGERRLWHVTSAGKVSDLLVAGEVGILVLDAQGESDTAAVFVAQIKRQFPDLVV
                     LVAGNREAENSLAALISAGTVYRFIHKPVSPGRAKLFADAAVKRYDAQRQRAAAAPPA
                     RPRVRSNRGWLIGGLVGALALSAAAAWMLHRGSDQAVAPPHALPGAPLESPLLAQAAA
                     ALTANRLTEPAGDNALELFLQAQARNPADQEARAGLAEVRERLLAKAENALLEERLDE
                     AAAAIETARKSGVEGGRISFLTAQLAKSRERVKSAQTKLRARADPKADDGKITSLLAI
                     AAQRLNDSQLAEPEGDSALFYIQQALQLDPKSPAAQAARDTLQTRLLSEFRAAIDRRD
                     FAHASTWLEAAKGFAAPDDIETAQQALSVARRRADTDAWAQLLKNGVERAQQDRLVEP
                     SNDSAKYYLLTLRGLDPNNAGLAPALQDLGSRLLAKAQRAVALEQFDAARSWLAEAAA
                     IGFASAESVSTQHDLDAAVAKQKFMANVVAANDLTLVKSVTPAYPKSAEAKKTQGWVE
                     LDFTVGLSGAVKDIVVHAASTPGVFDDAAISALAKWRYQPVVRDSQPKEQRARVRIRF
                     VLT"
BASE COUNT          338 a          686 c          680 g          377 t
ORIGIN      
        1 ggtcaggacg aagcggatgc gcacccgggc gcgctgctcc ttgggctgcg agtcgcggac
       61 gaccggctgg taacgccatt ttgccagggc gctgatggcc gcatcgtcga acacgcccgg
      121 ggtgctcgcg gcgtggacga cgatatcctt gaccgcaccg cttaagccga cggtgaagtc
      181 caattcgacc cagccctgcg tttttttcgc ttcggcgctc ttcgggtaag ccggcgtcac
      241 cgacttgacg agtgtcagat cgttggccgc gaccacgttg gccatgaact tctgcttagc
      301 gacggccgca tccaggtcat gctgcgtcga aacggactcg gcggaggcaa agccgatcgc
      361 ggccgcttcc gccagccagc tgcgagccgc atcgaactgc tcgagcgcca cggcgcgctg
      421 cgctttcgcc agcagccggc tcccgagatc ctgcagcgcg ggagcgagac ccgcattgtt
      481 gggatcgagc ccgcgcagcg tcagcaaata atacttggcg ctgtcgttgg acggttcaac
      541 caggcgatcc tgctgcgctc gctcgactcc gtttttcagc aactgcgccc acgcgtcggt
      601 gtcggcccgg cggcgcgcga ccgaaagggc ctgttgcgcc gtttcaatat cgtccggggc
      661 ggcaaaaccc ttggcggcct ccagccacgt cgatgcgtgg gcgaagtcgc gacgatcgat
      721 cgccgcacgg aattcgctca ggagccgcgt ctgcaacgtg tctctggcgg cctgtgcggc
      781 gggacttttg ggatcgagct gcaaggcttg ctggatataa aagagcgcac tgtcgccctc
      841 gggttccgcc agctgcgaat cattcaaacg ctgtgccgcg atcgcgagca aggaggtgat
      901 cttgccatcg tcggctttgg ggtcggcgcg tgcgcgcagc ttggtctgcg cgcttttcac
      961 ccgctcgcgg gacttggcaa gctgagcggt caagaaagaa atgcggccgc cttcaacccc
     1021 cgacttgcgc gccgtctcga tcgccgcggc ggcttcatcg agacgctcct cgagcaaggc
     1081 attttccgcc ttggcgagca ggcgctcgcg cacttccgcg aggccggccc gggcctcttg
     1141 gtcggcgggg tttcgcgcct gagcttgcag aaacagctcg agcgcattgt cgccggcggg
     1201 ctcagtcagc cggttggcgg tgagcgcggc cgccgcctga gcgagcagcg gtgattcgag
     1261 cggcgcgccg gggagtgcgt gcggcggcgc gaccgcctga tcggagcccc ggtgaagcat
     1321 ccaggccgct gccgccgaca aggccagggc accgaccaga ccgccgatca gccagccgcg
     1381 attcgatctc acccggggtc ttgccggcgg cgcagcggcg gcgcgttggc gttgtgcgtc
     1441 gtatctcttg accgcggcgt cggcaaacag cttggcccgg cccggcgaga ccggcttgtg
     1501 gatgaatcga tagacggtac cggcgctgat caaagccgcc agagaatttt ccgcctcccg
     1561 attgccggcg accaagacca cgagatcggg gaactggcgc ttgatctgtg ccacgaatac
     1621 cgcagcggta tcgctctctc cctgcgcatc gagcaccaga atgcccactt cgccggccac
     1681 cagcaggtcg ctgaccttcc cggccgacgt gacgtgccag agccgccttt cgccgccgac
     1741 cgcctcgcgc agggtttgca gaaatatctc atcgctggtg agcaccacga gctcgacgat
     1801 ctcgctcgca aggctgtagc ggagcgcatc caaggtggcg ctctcttcgt ccgaagccag
     1861 caccggttgt ttccctctca ttggaaccct tcacattgct caggccagtc gcggtcgacc
     1921 gcactgcagc agcggaatgc cacattactc aggctagtgt cattccacgg gcgacgctat
     1981 acgatacgga gtctcgggcg gcagacaacg tctgttgcgt tgcgtcaagc cgattttgtg
     2041 actggacact cattcaggag gcgcgcgcgc tcatggcggg g
//
LOCUS       DASWZT010000561         4741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_86724, whole genome shotgun sequence.
ACCESSION   DASWZT010000561 DASWZT010000000
VERSION     DASWZT010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86724"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VGE92_16075"
     CDS             <1..883
                     /locus_tag="VGE92_16075"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY1315401.1"
                     /translation="PSAGHVQVPIWPVAAPDAIPDPNPETVVPGGGAHDVSRPTMTVY
                     AANGRNTGAAVVVFPGGGYRSLAMDGEGTEICDWLTSRGITCVLLKYRVPGSGPWWDT
                     ENKRRVYPKVQTALQDAQRTLGLVRQRAAQWHVDPRKIGVMGFSAGGHLVAAVSTHFA
                     KRTYAPVDAADQLSCRPDFAIAVYPGHLWVHEDEDEDSRNEVDLSLRPDITVRADSPP
                     TFLLQAEDDRVDGVQQSLAYYVALQKAGVPTEMHLYAQGGHAFGLRKSNLPIAQWPRL
                     VETWLGTNGIAVAALTR"
     gene            complement(912..1745)
                     /locus_tag="VGE92_16080"
     CDS             complement(912..1745)
                     /locus_tag="VGE92_16080"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEY1315402.1"
                     /translation="MLPTDLQNTPSRQDAAAGGTEPGLLVAERRHRIVELLREHGKVT
                     VEALAARFATSAVTIRTDLAALEAERALERTHGGALLRQDDDDQPLIVKRTLHHAEKV
                     RIAKAAAALIQDGETVILDSGTTTAEIAKQIRKLEVRSINVITNALNVAMILAEVPSV
                     RLIMPGGILRPESNSLSGHMAEAALADLHADRLFLGADGLDPERGVMTPHLPEALLNA
                     RMIAISRQVIAVADSSKLLRRNISLIARVEQLHMLITDSGANPAVVAELRRRGVEVRL
                     A"
     gene            complement(1751..3847)
                     /locus_tag="VGE92_16085"
     CDS             complement(1751..3847)
                     /locus_tag="VGE92_16085"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 36 protein"
                     /protein_id="HEY1315403.1"
                     /translation="MTQRKTSRRQFVQFAGGLALALRHGLLPAQTETETTTPKPRAMA
                     ALEDAALRIEWDANLYTRVSRTVGNRRIPMTLWGPSDYLQSEPARHIAAFAIRHQAQA
                     EIDDANGPGTRLTLSGTSADGVEKIVEATLYRRHPGIALIRVSYRNAAAQILSFRSWT
                     NGDFRLPASGARAPQFWCYSGATYEDRRDWVQAVEPGFAQDNFLGMEASDYGGGTPIV
                     DVWRRDGGLAVGHVESTPRRVSLPVQAERRTVRVAVCGREKRDLRPGDLFHTPQTFVA
                     VHDGDYFSLLSAYRRLMSERGLTPAVPPQASYEPIWCAWGYERACTTTLIEGTLPKVK
                     DLGLEWAVIDDGWQAMIGDWNPHRAKYPNGEADLRHLVSDIRAGGLKPRLWYSPLSVA
                     PGSDFLHDHADMLLLDKDGAVQNISWWNSFYLCPAYQPTVLYTQGLIKKFIGEWGFAG
                     LKIDGQHLNNVAPCFNPAHHHARPEESVEGLQDFFRAIYQTAMQINPDAVIEVCPCGT
                     AYSVFNFPYLNQAPASDPESSWQVRHKGKTLKALMGPSAPYAGDHVELSDGHDDFAST
                     VGVGAVVSTKFTWPEDPKPKDSFLLTPEKERAWRRWIALYKEKMLPLGIYRGELYDIG
                     FDRPETHIIEKDGRLYYAFFADRWSGPVALRGLQAGPYRIRDYFNERDIGTVSGAPAR
                     LAVKFERFLLLEAIPA"
     gene            4019..>4741
                     /locus_tag="VGE92_16090"
     CDS             4019..>4741
                     /locus_tag="VGE92_16090"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY1315404.1"
                     /translation="MPSLKATLIAIWLPFAATGAIAADLDATAAADSAAAVEPAGKNV
                     PDTELQEIVVTGLRASLEKSLDIKKHAAVVLDSINAEELGRFPDADVADSLEHLPGIT
                     ISRTTGGEGQKVSVRGFGPQYNIVTLNNRILATDDDARDLAFDVLPSEVISGADVLKS
                     SEASALEGSIGGTVNLRTASPFDNLGFHGGAHTEGNYNEMTSLHGYKVSAFASDTLLD
                     NTLGFLIGGVLSDTHTRTDALNA"
BASE COUNT          874 a         1498 c         1520 g          849 t
ORIGIN      
        1 accatctgcg ggtcatgtgc aggttccgat ctggcccgtc gcggcacctg atgcgatacc
       61 cgacccgaac ccggagacgg tagttcccgg cggaggcgcg cacgacgtca gccggcccac
      121 aatgacagtg tatgcggcca acggacgcaa caccggcgcg gcagtggtgg tatttcctgg
      181 cggcggctac cgcagtctcg ccatggatgg tgagggcaca gagatctgcg actggctgac
      241 atcacgcggc atcacctgtg tactgctgaa ataccgcgtg ccgggctcgg gtccgtggtg
      301 ggacaccgag aacaagcggc gcgtataccc gaaggtgcaa accgcgctgc aggacgcgca
      361 gcgcaccttg gggctggtgc gccagcgcgc ggcgcagtgg catgtcgatc ctcgcaaaat
      421 cggcgtcatg ggtttttccg ccggcgggca tttggtggcc gcggtcagta cgcatttcgc
      481 gaagcgcact tatgccccgg tggatgccgc cgatcagctg agctgccgac ccgacttcgc
      541 catcgccgtc taccccggcc acctgtgggt gcacgaggac gaggacgaag acagccgcaa
      601 cgaggtggat ttgagtctgc gtccagacat caccgtacgc gccgactcgc cgcccacctt
      661 cctgctccaa gccgaggacg accgcgtgga cggcgtgcag caatctctcg cctattacgt
      721 cgcgctgcag aaagccggcg tgcccaccga gatgcacctg tatgcgcaag gcggccacgc
      781 tttcggtctg cgcaagagca atttgccgat cgcgcaatgg ccgcggctgg tggaaacatg
      841 gttggggacc aatggaatcg cggtcgcggc cctcacacgc tgaccgaagg caacgcgagc
      901 atgcgccgcc ctcacgccag ccgcacttcg accccgcgac gtctgagttc agcgaccacc
      961 gcgggattcg cgcccgagtc ggtgatcagc atgtgcagct gttcgacccg ggcgatgagc
     1021 gagatgtttc ggcgcagcag cttggaggaa tcggcgaccg cgatgacctg gcgcgagatc
     1081 gcgatcatcc tggcattgag cagtgcttcg ggtaggtggg gcgtcatcac gccgcgctcg
     1141 ggatcgaggc cgtcggcgcc gagaaacaga cgatcggcgt gcagatccgc cagcgccgct
     1201 tcggccatgt ggcccgatag cgagttcgat tcggggcgca gtatgccgcc cggcatgatc
     1261 agccgcaccg aagggacctc ggcgagaatc atggcgacat taagggcgtt ggtgatcacg
     1321 ttgatcgacc ggacttcaag cttgcgaatc tgcttggcga tctcggcggt ggtggtgccg
     1381 gaatcgagga tgaccgtctc gccgtcctga atcagcgccg ccgcggcctt ggcgatgcgc
     1441 accttctccg catggtgcag ggtgcgcttg acgatgagcg gctgatcatc gtcatcctgg
     1501 cgcagcagcg cgccgccatg cgtgcgttcg agcgcacgtt ccgcttcgag tgccgcaaga
     1561 tcggtgcgga tggtgaccgc cgaggtcgcg aagcgcgccg ccagcgcttc cacggtgacc
     1621 ttgccgtgct cgcgcagcag ttccacgatg cggtggcggc gctccgcgac caagagtccg
     1681 ggctcggtgc cgccggcggc ggcgtcctgg cgcgacgggg tgttttgcaa atctgtgggc
     1741 agcatcgaac ttaggccggg atggcttcga gcagcaagaa gcgttcgaat ttcaccgcca
     1801 gccgggccgg cgcgccggac acggttccga tgtcgcgctc gttgaaatag tcgcggattc
     1861 ggtacggccc cgcctgcagc ccgcgaagcg ccacggggcc gctccagcgg tcggcgaaga
     1921 acgcgtagta cagccggcca tccttctcga tgatatgcgt ctccggacga tcgaaaccga
     1981 tgtcatacag ctcgccgcga taaatcccaa gcggcagcat cttctccttg tagagcgcaa
     2041 tccagcggcg ccatgcgcgc tctttttccg gcgtgagcag gaacgaatcc ttcggtttcg
     2101 gatcctccgg ccacgtgaac ttcgtcgata ccaccgcgcc gacgcccacg gtggacgcaa
     2161 aatcatcgtg gccgtcgctg agctcgacgt gatcgccggc gtagggggcg ctaggcccca
     2221 tcaatgcctt cagcgtcttg cctttgtggc gcacctgcca agaagactcc gggtcggagg
     2281 ccggcgcttg attcaggtag ggaaaattga acaccgaata ggcggtgccg cacgggcaga
     2341 cctcgatcac tgcgtcgggg ttgatctgca tcgcggtttg gtaaatcgca cgaaagaaat
     2401 cctgcaagcc ttcgacggat tcctccggac gggcatggtg atgtgccgga ttgaagcacg
     2461 gtgcgacgtt gttcaagtgc tggccgtcga tcttgagtcc ggcgaatccc cactcgccga
     2521 tgaacttctt gatcagacct tgcgtgtaca acacggtcgg ctggtaggcg gggcagaggt
     2581 agaagctgtt ccaccaggaa atattttgga ccgcaccgtc cttatcgagc agcagcatgt
     2641 cggcgtgatc gtgtaagaag tcggagcctg gcgcgaccga cagcggggaa taccagaggc
     2701 gcggcttcag gccgccggcg cggatgtccg acacgagatg gcgcaggtcc gcttcgccat
     2761 tgggatactt ggcgcgatgc ggattccaat caccgatcat cgcctgccag ccatcatcga
     2821 tgaccgccca ttccaagccc aagtccttca ccttgggcag cgttccctcg atcaacgtcg
     2881 tggtgcaggc gcgctcatag ccccacgcac accaaatcgg ttcgtaggac gcttggggag
     2941 gcaccgccgg cgtcaagccg cgttcggaca tgaggcgtcg gtaggcactc aacagggaaa
     3001 agtagtcgcc gtcgtgcacc gccacgaagg tctgcggcgt gtgaaaaagg tcgccgggcc
     3061 tcaagtcgcg cttttcgcgt ccgcacaccg ccacgcgtac cgtgcggcgc tcggcctgca
     3121 cgggaagcga cacgcgtcgc ggcgtgctct ccacgtgacc caccgcgagc ccgccgtcac
     3181 ggcgccagac atcgacgatg ggcgtgccgc cgccgtaatc ggaggcctcc atgccgagaa
     3241 aattgtcctg cgcgaagccc ggttcgaccg cctgcaccca atcgcggcga tcctcgtacg
     3301 ttgcaccgga atagcaccag aattggggtg cgcgcgcgcc ggaggcgggc agccggaaat
     3361 cgccattggt ccagctgcga aagctcagaa tctgcgctgc agcgttgcgg tacgagacgc
     3421 ggatcagcgc gatgccagga tggcgccgat acagcgtcgc ttcgacaatt ttctcgacgc
     3481 cgtccgcgga ggttccggac aatgtcagcc gcgtgcccgg accgttggca tcgtcgattt
     3541 cggcctgcgc ctgatgacgg atcgcaaatg cggcgatgtg tcgagccggc tcgctttgca
     3601 ggtaatcgct cggtccccag agagtcatcg gaatccggcg gttgccgacg gtgcgcgata
     3661 cgcgcgtgta gagattcgca tcccactcga tacgcagcgc tgcatcttcg agggcggcca
     3721 tcgcgcgcgg cttgggtgtg gtcgtctcgg tctcggtttg cgccggcaac agtccgtggc
     3781 gcagcgccag cgcgagtccg ccggcgaact gcacgaactg tcgacgcgaa gtctttcgct
     3841 gagtcatgga tctttgatca tgaaccgatg cgttgtgcct attgacttgt tctcgaaaat
     3901 aaccataaca ttaaacgcaa gaatacgaaa gcaattaccg ggattgatgt ttcatttgct
     3961 ttcgttttca cttcggcgac atcaataaac catgctcaat ggtacaggga gacgacgcgt
     4021 gccaagcctc aaggctactt tgattgcgat ttggctgcca ttcgcagcga ccggcgcgat
     4081 cgccgctgac ctcgatgcga ccgccgccgc ggactccgcg gcagcggtgg aacccgccgg
     4141 caagaacgtc ccggatacgg agcttcagga gatcgtcgtc acgggtcttc gggcaagcct
     4201 cgagaagtcc ctcgacataa agaagcacgc cgccgtggtg ctcgattcca tcaatgccga
     4261 ggagctcggc cgatttcccg acgccgacgt cgccgactcg ctggagcatt tgcccggcat
     4321 caccatcagc cgcaccaccg gcggcgaggg gcagaaggtc tcggtgcgcg gcttcgggcc
     4381 gcaatacaac attgtcacgc tgaataaccg gattctggcc accgacgacg atgcgcgcga
     4441 cctggcgttc gacgtgctgc cctcggaagt gatctccggc gccgacgtgc tcaagtcctc
     4501 ggaagcctcc gcgctcgagg gcagtatcgg cggcacggtg aacctgcgca ctgctagtcc
     4561 gttcgataac ctgggcttcc acggcggcgc gcataccgag ggcaattaca acgaaatgac
     4621 ctcgctgcat ggctacaaag tctcggcgtt cgcctcggac acgctgctcg acaacacctt
     4681 gggattcctg atcgggggcg tgctgtccga tacccacacg cgcaccgatg cgctgaacgc
     4741 c
//
LOCUS       DASWZT010000562         2216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_67370, whole genome shotgun sequence.
ACCESSION   DASWZT010000562 DASWZT010000000
VERSION     DASWZT010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67370"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="VGE92_16095"
     CDS             <1..1154
                     /locus_tag="VGE92_16095"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEY1315405.1"
                     /translation="TEQGAPLGLPQRQLLARRIEDEVLGLGPLEPLLNDPTVSDILVN
                     GAKSVYIERRGRLEATTITFHDDAHLMNIIDRIVSAVGRRIDESAPMVDARLADGSRV
                     NAIIPPLAIDGPMLSIRRFAVERFQMADLLRFGTITEPVAKCLRAVVLGRLNVLISGG
                     TGAGKTTLLNVLSGFIPATDRIVTIEDSAELQLQQPHVVRLETRPPNIEGKGEVPQRE
                     LLRNSLRMRPDRIVVGEVRGGEALDMLQAMNTGHDGSLTTVHANTPRDALGRIETMIS
                     MAGIAFPIRALRAQIASAINLVIQVQRGDDGKRRVVSVQEISGMEGDMVTMSEIFTFR
                     RVGTDANGLIQGRLEATGAVPAFHQRVAVRGIDLPVSVFALPDGRFRNA"
     gene            1157..2131
                     /locus_tag="VGE92_16100"
     CDS             1157..2131
                     /locus_tag="VGE92_16100"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY1315406.1"
                     /translation="MNSNTAVIGFLCVVFAAVFVLMLGITNPASGNARQQRTLRRRLA
                     AIGADAKEDVASLLREKYLTSLTPAERRLEELPLMEGLAQMSEQAGKPSPGYRVVFSS
                     VLLGAVLGVVSGALLHALPIGIAAAVAGLLIPYLRLSMLRRQRMNTIEEQLPDAIDVI
                     KRALRAGHPFNSAIKLVADDMDGPIAQEFGLMFTDLNYGSDVRRALLGLLNRMPSVTV
                     MALVTSVLVQRETGGNLAEILDQIGKVVRSRFRLGRRIRSLSAEGKMSAWVLAMVPVA
                     LAGLMSFTAPTYLPILFTDPLGHKMLYGAGTLGVIGVLWIRKIIRIEV"
BASE COUNT          397 a          665 c          700 g          454 t
ORIGIN      
        1 ttaccgagca gggcgcgccg ctcggccttc cgcagcgcca gttgttggcg cgccgcatcg
       61 aggatgaagt gctcggactc gggccccttg agccgctgtt gaatgatcca accgtgtccg
      121 acattctggt caatggcgcc aagtcagttt acattgaacg ccgcggccgg ttggaggcga
      181 ccaccatcac attccacgac gatgcgcatc tgatgaacat catcgaccgc atcgtgtcgg
      241 cggtcgggcg ccgcatcgat gaatccgcgc ccatggtcga tgcgcgcctg gccgatggct
      301 cgcgcgtgaa cgcgatcatc ccgccgctgg ccatcgacgg gcccatgctg tcgatccggc
      361 gattcgcggt agagcgattt cagatggcgg acttgctgcg attcggcaca ataacggagc
      421 cggttgccaa atgcctgcgg gcggttgtgc tcggccggct taacgtactc atctccggcg
      481 gcactggcgc cggcaaaacg accctgctca acgttctgtc cggcttcatt cccgccacag
      541 accgcatcgt tactatcgag gattccgcgg aactgcagct gcagcagccg cacgttgtac
      601 gcttggagac ccggccgccg aacatcgagg gcaagggcga ggtgccacag cgcgaactgc
      661 tgcgcaacag tttgcgaatg cggcccgacc gtatcgtcgt cggcgaggtc cgcggcggcg
      721 aggcgctcga catgcttcag gcgatgaaca ccgggcatga tggttcgctg acgaccgtgc
      781 atgccaatac gccgcgcgac gcgctgggcc gcatcgagac catgatctcg atggccggaa
      841 tagcgttccc gatccgcgct ttgcgggctc agatcgcttc ggcgatcaat ctcgtgatcc
      901 aggtgcagcg cggcgacgat ggcaagcgac gcgtggtcag cgttcaagaa atcagcggca
      961 tggagggcga catggtcacc atgtcggaaa tctttacgtt ccgccgtgtc ggaaccgacg
     1021 ccaatggcct gattcaaggg cgactcgagg cgacgggtgc cgtcccagct ttccatcagc
     1081 gagtcgcggt gcgcggcatc gatctgcccg tcagcgtttt tgcactgccg gacggccgtt
     1141 ttaggaacgc gtaagcatga atagcaacac ggcagtcatc ggcttcctct gcgtagtgtt
     1201 cgccgctgtc tttgtgctca tgctcgggat cacgaatccc gcgagcggca acgcgcgcca
     1261 gcagcgcacc ttgcgccgcc gtttggccgc catcggcgca gacgcaaaag aggatgtggc
     1321 ctcgctgttg cgcgaaaagt acctgacttc gttgacaccg gccgaacggc ggctcgaaga
     1381 actgccgctc atggaaggcc tcgcgcaaat gagcgagcag gccgggaaac catcgccggg
     1441 ctatcgtgtg gtgttctcat cggtgctgct gggtgcggtg ctcggcgtcg tgtccggcgc
     1501 cttgctgcat gccctgccca ttggtattgc ggcggcggtc gcaggtcttt tgatccctta
     1561 cctgcgcctc tcgatgctgc ggcggcaacg gatgaatacg atcgaagagc agcttcctga
     1621 cgccatcgat gtcatcaagc gcgcgctgcg tgcgggccac ccgttcaact cggccatcaa
     1681 actcgttgca gacgacatgg acggtccgat cgcgcaagaa ttcggactca tgttcaccga
     1741 cctcaattac ggcagcgatg tgcggcgcgc cttgctcgga ttgttgaatc ggatgcccag
     1801 tgtgactgtg atggcactcg tgacctccgt gctggtgcag cgcgagacag gcggcaactt
     1861 ggcggagatt ttggatcaaa tcgggaaggt cgtgcgtagc cgtttcagac tcgggcgcag
     1921 aattcgttcg ctatccgccg aggggaaaat gtcagcttgg gttctcgcca tggtgccggt
     1981 ggcactggcg ggcttgatgt cgtttaccgc gcctacctac ttgccgattt tgttcaccga
     2041 tccgctgggc cacaaaatgc tgtacggcgc gggcaccttg ggagtcatcg gcgtcttgtg
     2101 gattcgaaaa ataattcgga tcgaggtcta agtgcatgct tgataatctt ctcaacggca
     2161 atcccactgt ggtcatgatt ctggcggctg cagccgcatt ggtgtttgtc tgggga
//
LOCUS       DASWZT010000563         2550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_91560, whole genome shotgun sequence.
ACCESSION   DASWZT010000563 DASWZT010000000
VERSION     DASWZT010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91560"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1423)
                     /locus_tag="VGE92_16105"
     CDS             complement(<1..1423)
                     /locus_tag="VGE92_16105"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY1315407.1"
                     /translation="MMHRRAAYAGEWLDRSRDLKFSFEGREYTGFSGDTISSALAAAG
                     PALLARSFKYHRPRSILSFANHDSNTLFQVDGVPNVRGDVTLLREGMRVSALNTFGGL
                     ARDRARVLDRLAPLLPVGFYYKAFHSKRLFPRWERMFRRLTGLGAVTLSAEREASAKR
                     YGFCDVLVIGAGPSGLSAALSAAQSGAQVSLVDESLRIAGNGAPPELLQAVQACAAIT
                     LHPATVAVGYYADHWVALASPTYLTKMRAKAVVCATGVIEQPAVFRNNDLPGVMLASG
                     AVRLLTRYGVAPGHRVVMIAANLEAYLTCLELSARGVVVAAIIELRAAPDSTAPLADC
                     VARGMLLLAGHAPYEALRARDGSVAGLNVAPLEAQAQVKGRALRRIDCDAILMSVGWA
                     AATQLVLQAGGSTRFAEELQQFVPDALPPGIFAAGRMNGVYDEARLADGRRAGSQAAA
                     HAGFGLPNGLPNGLPNGSPFGERI"
     gene            complement(1420..1707)
                     /locus_tag="VGE92_16110"
     CDS             complement(1420..1707)
                     /locus_tag="VGE92_16110"
                     /inference="COORDINATES: protein motif:HMM:NF016179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEY1315408.1"
                     /translation="MKIMACPLNGPRPISEFAYGGEVRPSPDPDTCSDLEWSNYVFNR
                     SGSPGLKYEWWCHIASGFWFVVLRDTTRDVVLDTLSAEAVRRGLPVPGGAA"
     gene            complement(1707..>2550)
                     /locus_tag="VGE92_16115"
     CDS             complement(1707..>2550)
                     /locus_tag="VGE92_16115"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1315409.1"
                     /translation="VNKHYGVRSEAVDAAFVKRGIPCIDMSCGGHAPILGALFHEPGA
                     IARHDAVAWGYGRGADRRGVEIHQQTEVTGIRTAGGRVVGVDTTRGAIATSKVLCAVA
                     GFTPRILDMVDLHTPIYVHPLQAMVSEPVKPWLDPIIVSGSLHVYVSQSARGELVMGA
                     SLDPYELHSARSTLDFVEGLAAHMLDMFPFLSEVKVNRQWAGLADMTPDFAPIMGVTP
                     VEGFFLDAGWGTWGFKATPISGKTMSHTVASGRNHPLIEGFTFDRFARYQLTGEKGAA
                     SVGH"
BASE COUNT          476 a          877 c          803 g          394 t
ORIGIN      
        1 cgatgcgctc gccgaacggc gagccgttcg gcaagccgtt cggcaagccg ttcggcaagc
       61 cgaagcccgc atgcgccgcc gcctgcgaac cggcgcgccg gccgtccgcg agccgcgctt
      121 catcgtagac gccattcatc cggccggccg caaaaattcc cggcggcaag gcgtcgggaa
      181 cgaattgctg caattcctcc gcgaagcgcg tgctgccgcc ggcttgcagc acgagctgtg
      241 tcgcggcggc ccagccgacg ctcatcaaga tggcatcgca atcgattcgc cgcagcgcgc
      301 ggcccttgac ttgggcctgt gcctcgagcg gcgccacgtt gagtccggcg acactgccgt
      361 cccgtgcccg caaggcctcg tacggcgcgt gtccggcgag cagcagcatg ccgcgcgcga
      421 cgcagtctgc cagcggcgcg gtgctatcgg gagccgcgcg cagctcgatg atggcggcca
      481 ccaccactcc gcgcgcgctc aactcgaggc aggtcaggta cgcctcgaga ttcgcggcaa
      541 tcatgacgac ccggtgtccg ggagcgacgc catagcgcgt gagcagccgc actgcgccgg
      601 acgccaacat cacccccggc aaatcgttgt tgcgaaaaac ggccggctgc tcgatgactc
      661 ccgtggcaca caccactgcc ttggcgcgca ttttcgtcag gtacgtgggc gaggccagcg
      721 ccacccaatg atcggcgtag taacccaccg ccaccgtcgc cggatgcagc gtgatggccg
      781 cgcaggcttg caccgcctgc agcaactctg gcggtgcgcc attgccggca atacgcagcg
      841 actcatcgac caggctcact tgcgcgccgc tttgcgccgc ggatagcgca gccgacaggc
      901 cgctcggtcc cgcaccgatg accagcacat cgcaaaatcc atagcgcttg gcgctggctt
      961 cgcgctcggc gctgagcgtc acggcaccca atcccgtgag ccggcgaaac atccgctccc
     1021 agcgcgggaa caagcgcttg gaatgaaacg ccttgtagta gaaacccacc ggcaacagcg
     1081 gtgccaagcg atcgagcacc cgcgcgcgat cgcgtgccaa tccgccgaaa gtattgagcg
     1141 ccgagacgcg cattccctcg cgcagcaagg tcacatcgcc ccgcacattc ggcacgccgt
     1201 cgacctggaa cagcgtgttg ctgtcgtggt tggcgaatga caggatgctg cgcggtcgat
     1261 ggtatttgaa acttcgcgcg agcagcgccg gccccgcggc ggcgagtgcc gaggaaatgg
     1321 tatcgccgct gaagcccgtg tattcacggc cctcgaagct gaatttgagg tcgcgcgagc
     1381 gatcgagcca ctcgccggcg tacgcggcgc gacgatgcat catgcggcac cgcccggcac
     1441 cggcaagccg cgccgcacgg cttcagccga cagggtgtcg agcaccacat cgcgtgtcgt
     1501 gtcgcgcaac accacgaacc agaatcccga ggcaatgtga caccaccact cgtacttgag
     1561 tccgggcgaa ccggagcgat tgaatacgta attgctccac tccaggtcgc tgcacgtgtc
     1621 aggatcgggg ctgggacgaa cttcgccgcc ataggcgaac tccgagatcg gtcgcgggcc
     1681 gttcaagggg catgccatga tcttcatcag tgacccaccg atgccgcgcc tttctcgccc
     1741 gtgagctggt agcgcgcgaa gcgatcgaag gtgaagccct cgatcagagg atgattgcgc
     1801 ccgctcgcca cggtgtggct catggtcttg cccgagatgg gcgtcgcctt gaatccccag
     1861 gtgccccacc ccgcgtccag aaagaaaccc tcgaccggcg tcacgcccat gatgggcgcg
     1921 aaatccggcg tcatgtccgc aagcccggcc cactgccgat tgactttgac ttcgctcaag
     1981 aacggaaaca tatcgagcat atgcgccgcc agaccttcga cgaagtcgag cgtcgagcgg
     2041 gcggaatgca attcgtacgg gtcgagcgag gcacccatga ccagttcgcc gcgggcgctt
     2101 tgcgaaacgt acacgtgcag cgaacccgac acgatgatgg ggtcgagcca cggcttcacc
     2161 ggttccgaga ccatcgcctg cagcggatgc acgtagatcg gcgtgtgcag atccaccatg
     2221 tcgagaattc tcggtgtgaa tccggcaacg gcgcacagca ccttcgaggt cgcgatggcg
     2281 ccgcgcgtgg tgtcgacgcc cacgacccga ccgccggccg tacggatgcc ggtgacttcc
     2341 gtttgttgat gaatctcgac accgcggcga tcggcgccgc ggccgtagcc ccacgcgacc
     2401 gcatcgtggc gcgcgatggc cccgggttca tgaaacaacg cgcccagaat cggtgcgtga
     2461 ccgccgcagg acatgtcgat gcaaggaatg ccgcgcttca cgaatgcggc atcgaccgcc
     2521 tccgagcgaa cgccgtagtg cttgttgacc
//
LOCUS       DASWZT010000564         8721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_9466, whole genome shotgun sequence.
ACCESSION   DASWZT010000564 DASWZT010000000
VERSION     DASWZT010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8721
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9466"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="VGE92_16120"
     CDS             complement(<1..1154)
                     /locus_tag="VGE92_16120"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY1315410.1"
                     /translation="MNASAETWKSYAQLLARLIPKVSHILFASADGALWWSSDPRSAS
                     RVQYALSLLQNPQASRHEVDGLVETLDSAESRYGFRIRGALGELLGLVVIALPLPEAR
                     LELREVHALIKPALDCLQSELSARSAARIASGEPDLQQRLTDSAGADGWESLGQIPEL
                     ANQHLSGIVAAILLPDRNLTLCHKRADQPSGIESGVLAQMHRHLTTRAQLHGRTLVAN
                     HLALDGTNAAAPYKAISTPIRDETRRVIGVLAVFRAAGDGDFQLRDAEALEVLARKAA
                     QIACLSFDPVTGLLTAAAFRAQAGKLAARELRNHTHGLLYVDIDQLNVVNENHGMHVG
                     DEVIQGVANLLARRAREGTLVARVGGDRFSMLVPGCGIEPTARIAEELRSA"
     gene            complement(1301..2479)
                     /locus_tag="VGE92_16125"
     CDS             complement(1301..2479)
                     /locus_tag="VGE92_16125"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY1315411.1"
                     /translation="MRLNAARRARLVCAAALITSYCAGGWAAAPAQPRDSAQANSKLA
                     AVRARIAALTNRLGDELKQRDALSARLREADLAITTQRQRVDSLRIEQAAVERHRTEL
                     RSEQQDAQAALREQRAALAAQVRAAYMIGRQEQLKLLLNQSNPASLGRTLTYYGYFAE
                     ARSAKIESIQAQMSRLQQLVLEIDEQSAQLLALQDAAAREMANLAHARAERADALAAL
                     SKQVLSGNQELADLKREEQAVESLVAELARVMQDFPVDPSQSFDAMRGKLPWPVPGRV
                     TARYQEQHGNSAPGVRWNGEMIEAARGAKVRAPYFGRVVYADWLQGLGLLLIIGHSGG
                     YMTLYGHAEVLYKSVGDWVAPGDVIAALSDANAAPQLYFEIREGRKTVDPKAWLRTNP
                     "
     gene            2549..2998
                     /locus_tag="VGE92_16130"
     CDS             2549..2998
                     /locus_tag="VGE92_16130"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY1315412.1"
                     /translation="MQRLLEFIGNHYLLATGAILLAGAVAFYEIRERMQAFAALSSMQ
                     AVRLMNQGALVLDLRGKELYDAGHIGDARNVPVAELESQAESLKKWRDKNVITYCDSG
                     SNGALAARTLMKLGFTKVFNLQGGLSAWVKDNLPLAKSAAAGKGGAK"
     gene            2995..3261
                     /gene="grxC"
                     /locus_tag="VGE92_16135"
     CDS             2995..3261
                     /gene="grxC"
                     /locus_tag="VGE92_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018929204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEY1315413.1"
                     /translation="MSAPSVTLYVSDWCPYCQRAKGLLAQKNVTVNEINVEEQSKFRE
                     EMIARSHRRTVPQIFIGDKHVGGCDDLFELDRSGELDRLLQGAC"
     gene            3261..3737
                     /gene="secB"
                     /locus_tag="VGE92_16140"
     CDS             3261..3737
                     /gene="secB"
                     /locus_tag="VGE92_16140"
                     /inference="COORDINATES: protein motif:HMM:NF014599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEY1315414.1"
                     /translation="MNEVRTAAPEATIGGVQITLQSVYLKDCSYESPNGPRLPNNQAW
                     EPKFQLNMNTSAEELAADVREVLLTITLEAKQGEATLYLVEVKQAGVFAIAGASVEDL
                     KRLIGSFCPSVLFPYAREVISDLIVKGGFPNFLLPLVNFDALFAQASETPQTGLVN"
     gene            3750..4760
                     /locus_tag="VGE92_16145"
     CDS             3750..4760
                     /locus_tag="VGE92_16145"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY1315415.1"
                     /translation="MQRSSAEPMTVIGAGSWGTALAIQLAREGHPTRLWGRNGARLDA
                     MRLARRNDYYLPGAEFPDALQVTNELQQALGGARDALIAVPSHAFRETLESVKPHLDA
                     DTRIAWATKGFEIATGLLPHQVARQVLGARPGAVLSGPTFATEVGAGLPTAMTVASHD
                     ERFAKEMALSLSGPHFRAYTQADIMGVEVGGAVKNVIAIGCGIADGMGFGANTRVALI
                     ARGLAEMMRLGVKLGAARETFMGLAGLGDLVLTCTDDQSRNRRFGMALGRGRTVVEAQ
                     REIHQVVEGLPAARAVYEVAHRLQVDMPICQEIYRIMHEEKPVRAVVQALMGREVRAE
                     TD"
     gene            4772..5671
                     /locus_tag="VGE92_16150"
     CDS             4772..5671
                     /locus_tag="VGE92_16150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEY1315416.1"
                     /translation="MTTDVFTDRMFGGNPLAVMLDAAGLSTREMQAIALEFNYSETSF
                     VLPPDDEAHTAKVRIFTPRIEVPFAGHPNVGTAFALARESRFAAATEAGSFVFEEGAG
                     LVALELLREAGAVTGAELRAPEPLSRRSLVSREIAAACLSLDCADIAMTVHAPQIISV
                     GLPFLVVELESREALRLSKPNLAAHAQLLPLDGADAVFAYWRAPRDSAAAPAVVLHAR
                     TFSPLDGIVEDPATGSACAATIALLATLGAGPGEALAWRIHQGVDMGRPSLLLGRTSL
                     RDGRLAVHIGGRCIAVLEGTLNL"
     gene            complement(5666..6121)
                     /locus_tag="VGE92_16155"
     CDS             complement(5666..6121)
                     /locus_tag="VGE92_16155"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003171120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEY1315417.1"
                     /translation="MFHIVLFEPEIPPNAGNVIRLCANTGAALHLVKPLGFRLDAKSL
                     RRSGLDYHDLACVKVHAGLDDCLEELAGARLFAVETSGLQRYDQIAYRAGDAFIFGPE
                     TRGLAHSVVERTGRAASLFIPMRAASRSLNLSNAVALVAYEAWRQLGFL"
     gene            complement(6139..7230)
                     /locus_tag="VGE92_16160"
     CDS             complement(6139..7230)
                     /locus_tag="VGE92_16160"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY1315418.1"
                     /translation="MIGDYLAHKIDAAHQLYLSNSLTDWLIAGIVAIAVWSVLSIVRR
                     LVASRYKKYSAAQHRMPIRLLAYLVGNTRQFLFVALALDAAQTSLTLPDRVQRWVFNI
                     VLILVLLQVGLWAGRAVRFYLETKELERGADQVFAGSLDVINFVARVLIWSLLILVAL
                     DNLGVNITALLAGLGVGGVAVALALQNILGDLFASLSIALDKPFVVGDRLEIDAFGGK
                     VEHIGIKTTRLRSDAGEQIILSNADILKSRVRNFGRSLEQRILAAIRVTFDTPTATLR
                     EIPQVLEGIVRDQPTARFERCHLKTLGDSALLYELSYFVQQPSVNSLLDLQQAVNFRI
                     IDEFRRLGVEFDYPTQRVLVAQPRVAAEN"
     gene            complement(7259..8665)
                     /gene="glnG"
                     /locus_tag="VGE92_16165"
     CDS             complement(7259..8665)
                     /gene="glnG"
                     /locus_tag="VGE92_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEY1315419.1"
                     /translation="MSAKPLQVWLVDDDASIRWVLEKALKGSGMTAKCFDEADNVLSA
                     LRSGAPDVLMTDIRMPGRSGLDLLKEIQVSRPGLPVIVMTAHSDLDAAVAAYQGGAFE
                     YLPKPFDIDKAVDLVRRAAQQATVSIDVEAARRSIPELLGQAAAMQQVFRAVGRLSRS
                     SMTVLITGESGTGKELVARALHRHSPRSNRAFVALNTAAFTADLLESELFGHEKGAFT
                     GADALRRGRFEQADGGTLFLDEIGDMSPALQTRLLRVLAEGEFYRVGGQTPVKVDVRV
                     IAATHQDLEARVSSGHFREDLLHRLNVIRIEIPPLRERREDVPELLRHYLAVAGQELG
                     VEPKLLTSPAEMALVIYDWPGNVRQLVNACRRLTVTAPGREIRAEDIPPDLGGVPAGH
                     SASHDWMAKLSHWAEQELAAGTQRLLDQAVPEVERILIRVALRKADGLKQDAAKLLGW
                     GRNTLTRKMKELGMEDVT"
BASE COUNT         1579 a         2735 c         2839 g         1568 t
ORIGIN      
        1 gcgctgcgca gctcttcggc gattcgggcg gtcggctcga tgccgcatcc ggggaccagc
       61 atcgagaatc ggtcgccgcc gactcgcgcg accagcgtgc cctcgcgggc ccgccgtgcc
      121 agcaagttgg ccacgccttg aatgacctcg tcgcccacat gcatgccgtg attttcattg
      181 accacgttca gctggtcgat gtcgacatac agaagtccgt gcgtgtggtt gcgcaattcc
      241 cgagcggcca gtttcccggc ttgagctcgg aacgccgccg ccgtcagcag accggtcacc
      301 gggtcgaagc tcaaacaggc aatttgcgca gccttgcgcg ccagcacctc caatgcctcg
      361 gcatcgcgca gttggaaatc gccgtcgccg gcggcgcgga acacggcgag cacgccgatc
      421 acgcggcgcg tctcgtcgcg gatgggcgtc gagattgcct tatacggcgc cgcggcattg
      481 gtgccgtcga gggccagatg attggcgacc agggtgcgtc cgtgcagctg agcccgggtg
      541 gtcagatgtc gatgcatttg cgcgagcacg ccgctctcga tgccgctcgg ctggtcggcg
      601 cgcttgtggc aaagcgtcaa gttccggtcc ggcaggagga ttgccgcgac gattcccgag
      661 agatgttgat tggcaagctc cggaatctgt cccagggact cccagccatc ggcgccggcc
      721 gaatcggtca atcgctgctg caggtcgggt tcgccgctgg cgatgcgggc ggcggagcgc
      781 gccgacaatt cgctctgcaa acagtcgagc gccggtttga tcagcgcgtg cacctcgcgc
      841 agctcgaggc gcgcctcggg cagcggcaac gcgatgacga cgaggcccag caattcaccg
      901 agcgcgccgc ggatgcgaaa tccataacga gattcggcgc tatccagcgt ttccaccaag
      961 ccgtcgactt cgtggcgcga ggcctgcgga ttctgcagca gggaaagcgc atactgcacc
     1021 cgcgaagcgc tgcgcgggtc gctcgaccac cacaacgcgc catccgcgct cgcgaataga
     1081 atgtggctga cttttggaat caaacgcgcg agcaattgcg cgtacgactt ccaagtttcg
     1141 gccgaagcgt tcattgcgcc aagaatcggg caattgccgc ggcgaaacca ggacgcgctc
     1201 ggcacttcgc cggagatttt tagaaattaa tcggcagttt gccgcagacc ttgaatgcgg
     1261 acgcagttgc cgcatgcgac ataacgtcac ggcggcccgc tcaaggattg gtcctcaacc
     1321 aggctttggg atccacggtc tttcgccctt cgcgaatttc gaagtacagc tgcggcgcgg
     1381 cgttcgcgtc gctcagggcg gcgatcacat caccgggcgc aacccagtcg ccgaccgatt
     1441 tgtagagtac ttcggcatgt ccgtagagcg tcatgtagcc gccgctgtgt ccgatgatga
     1501 gcagcagacc gagtccttgc agccagtcgg cgtacacgac gcgcccaaag taaggtgcgc
     1561 gcactttggc gccgcgcgcc gcctctatca tttcgccgtt ccagcgtact ccgggcgcgg
     1621 aattaccgtg ttgctcctga tagcgagcgg tgacacggcc cggcaccggc cagggcagct
     1681 tgccgcgcat cgcgtcgaaa ctctgcgatg gatcgactgg aaaatcctgc atgacgcgcg
     1741 cgagttccgc gaccagcgac tcgaccgcct gttcttcgcg cttcaaatcg gcgagttcct
     1801 ggttgccgct caacacctgt ttgctcaatg ccgcgagcgc gtcggcgcgc tcggcgcgcg
     1861 cgtgcgccaa gttggccatc tcgcgggcgg cggcgtcttg cagtgccagg agttgcgcac
     1921 tttgttcatc gatttccagc accaactgct gcagccgcga catttgcgct tgaatgctct
     1981 cgatcttggc gctgcgcgcc tctgcgaaat agccatagta ggtgagggtg cgccccaagc
     2041 tcgccggatt gctctggttc agcaacagct tcagttgttc ctgccggccg atcatgtacg
     2101 cggcacggac ctgagccgcc agcgcggcgc gctgctcccg cagcgccgcc tgcgcgtcct
     2161 gctgctcgct gcgcagctcc gttcggtgcc gctccacggc ggcctgctcg atgcgcagcg
     2221 aatccacgcg ctgccgctgg gtcgtgatgg cgagatccgc ctcgcgcagg cgcgcgctca
     2281 gtgcgtcgcg ctgcttgagt tcatcgccga gacgattggt gagcgcggcg atgcgcgcac
     2341 gtacggccgc gagtttggaa ttggcctgcg cggaatccct gggttgcgcc ggggccgccg
     2401 cccagccgcc cgcgcaatac gatgtgatca gcgcggcagc acataccagt cgggcgcggc
     2461 gggcggcgtt aagtctcatg atggcaagca tacaagcctt gagcccgctg ctatactttg
     2521 cggctgttaa aaccttggcg cgagccgcat gcagcgactg ttggagttca tcgggaatca
     2581 ctacttgttg gcgaccggag cgatcctgtt ggccggcgcg gtggcgtttt atgagattcg
     2641 cgagcgcatg caggcgtttg ccgccttgag ttccatgcag gccgtgcggc tcatgaatca
     2701 aggcgcgctg gtcctcgatt tacgcggcaa ggaattgtac gacgccgggc acatcggcga
     2761 tgcgcgcaat gtacccgtgg cggagctgga gtcgcaggct gagtcgctga aaaagtggcg
     2821 cgacaagaac gtgattacct attgcgacag cggcagcaac ggcgcgctcg cggcgcgcac
     2881 gttgatgaag ctgggcttca ccaaagtctt caatctgcag ggcggtttga gcgcgtgggt
     2941 caaggacaac ctgccgctgg caaaatccgc tgccgccggc aaggggggcg cgaaatgagc
     3001 gcaccgagcg tgacgctgta cgtgtcggac tggtgtccgt actgtcagcg ggccaagggc
     3061 ttgctggcgc agaagaacgt gaccgtaaat gaaatcaatg tcgaggagca gtcgaagttc
     3121 cgcgaggaaa tgattgcacg cagtcatcga cgcaccgtgc cgcagatctt tatcggcgac
     3181 aagcatgtcg gcggctgcga cgatcttttc gaactcgatc gcagtgggga actggatcga
     3241 ctactccagg gggcttgttg atgaacgagg ttagaactgc tgcgccggag gccaccatcg
     3301 gcggcgtcca gatcacgctg caaagcgtgt acctgaaaga ttgttcctat gaatcgccga
     3361 atggtccgcg cctgccgaac aatcaagcct gggagcccaa gtttcagctc aacatgaata
     3421 cctcggccga ggaactcgca gccgatgtgc gcgaagtgct tttgaccatc acgctcgagg
     3481 cgaagcaggg cgaggcgacg ctgtacctgg tcgaggtgaa gcaggcgggg gttttcgcaa
     3541 tcgcgggagc ttcggtggag gatctgaaac gactgatcgg cagtttctgt ccgagcgtgc
     3601 tgtttcccta cgcgcgcgaa gtgatttcag atttgatcgt caaaggcggc tttcccaatt
     3661 tccttctgcc gttggtcaac ttcgacgcct tgtttgcaca ggcgtcggaa acgccgcaaa
     3721 ccggtctcgt gaactaaggg gccggaggga tgcagcgctc aagcgctgaa ccgatgacgg
     3781 tcatcggcgc gggttcctgg ggtaccgcgc tcgcgattca actggcgcgc gaagggcatc
     3841 caacccggct gtggggacgc aatggcgcgc ggctcgatgc catgcggctt gcacggcgca
     3901 acgactatta tctgccgggc gccgagttcc ccgacgctct gcaagtaacg aacgagctgc
     3961 agcaggcgct cggcggagcg cgcgacgcac tgatcgccgt gccgtcgcac gcatttcgag
     4021 aaaccttgga aagcgtcaag cctcacttgg atgccgacac ccgtatcgcc tgggccacca
     4081 agggcttcga gatcgccacc ggactcctgc cgcaccaggt tgctcggcag gtgttgggcg
     4141 cgcggccggg agcggtattg tccggtccca cctttgccac ggaggtcggg gccggcctgc
     4201 ccaccgcgat gacggtcgcc tcgcacgatg agcgctttgc caaagaaatg gcattgagcc
     4261 tgtcgggacc tcatttccgc gcctacacgc aagccgacat catgggcgtc gaggtgggcg
     4321 gcgcggtcaa aaacgtcatc gccatcggct gcggcatcgc cgacggcatg ggtttcggcg
     4381 ccaatacccg cgtggcgttg atcgcacgcg gcctcgccga gatgatgcgc ctcggcgtca
     4441 aactcggcgc tgcgcgcgag accttcatgg gcctcgccgg gctcggcgac ctggtgctga
     4501 cctgcaccga cgatcaatcg cgaaatcgcc gcttcggcat ggcgctcggc cgcggccgaa
     4561 ccgtggtcga agcgcagcgc gaaatccatc aagtcgtgga ggggctgccc gcggcgcgcg
     4621 cggtctatga agtcgcgcac cgcctgcagg tggacatgcc catttgccag gaaatttatc
     4681 ggatcatgca cgaggagaag ccggttcgag cggtggtgca ggcgctgatg ggccgtgaag
     4741 tgcgcgcgga gacggattga ggcgacgcta cgtgaccacg gacgtcttca ccgaccgcat
     4801 gttcggcggc aatccgctgg cggtgatgtt ggacgcggcg ggcctctcga cgcgcgagat
     4861 gcaggcgatc gcgctcgaat tcaattactc ggagaccagc ttcgtgctgc cgcccgatga
     4921 cgaggcgcac acggccaagg tgcgcatctt cacgccgcgc atcgaggttc cgtttgccgg
     4981 acacccgaat gtcggaacgg cgttcgcact ggctcgcgag agccgcttcg ccgccgccac
     5041 cgaggccggg agtttcgtct tcgaggaggg cgcgggtctc gtggcgctcg agctgctgcg
     5101 cgaggcgggc gcggtcaccg gcgcggagct tcgcgcgccg gagccgctgt cgcgccgcag
     5161 cttagtatcc cgcgagattg cggccgcgtg cctgtcgctc gattgcgcgg acatcgcgat
     5221 gacggtgcac gcaccgcaaa taatctccgt cgggctgcca ttcctggtcg tcgaactcga
     5281 aagccgcgag gcactgcgtc tctcgaagcc gaatctcgcg gcgcatgcgc aacttttgcc
     5341 gctcgatggc gcggatgcgg tattcgccta ctggcgcgcg ccgcgagatt cggccgctgc
     5401 acccgcagtc gttctgcatg cgcgcacgtt ctcgccgctg gacgggatag tggaagatcc
     5461 cgcgacgggg agcgcctgcg ccgcgaccat cgcattgctc gcgacccttg gggccggccc
     5521 cggcgaggcg ctggcgtggc gcatccatca aggcgtcgat atgggccgtc cgagtctgct
     5581 gctgggacgc acctcgctgc gcgatggcag gcttgccgtc cacatcggag gtcgttgcat
     5641 cgcggttctc gaaggcacgt tgaatttata ggaagccaag ctggcgccag gcttcgtagg
     5701 cgacgagggc gacggcattg gatagattga ggctgcggct cgcggcgcgc atcggaatga
     5761 acaaagacgc ggcgcgcccg gtgcgctcga cgacgctgtg cgcaagcccg cgagtctccg
     5821 gaccgaagat aaacgcatcg ccggcgcgat acgcaatttg atcgtagcgc tgcagcccgc
     5881 tggtctcgac cgcgaacagc cgcgcgccgg cgagttcttc gaggcagtcg tccaagccgg
     5941 cgtgcacttt gacgcaggcc aggtcgtgat agtcgaggcc tgagcgccgc aggctcttgg
     6001 catccaggcg aaaccccaac ggcttgacca agtgcagcgc cgcgccggtg ttggcgcaca
     6061 ggcgtatgac gttgcccgcg ttcggcggta tctcgggttc gaacagcaca atgtgaaaca
     6121 cgaaggcggc ggccggcttc aattttcggc ggcgactcgc ggttgcgcga cgagtacgcg
     6181 ctgcgtcgga tagtcgaatt cgacgcccaa gcggcgaaac tcgtcgatga tgcgaaagtt
     6241 gacggcctgc tgcagatcga gcagcgaatt gacgctgggt tgctgcacga agtacgacaa
     6301 ctcgtacagc aatgccgaat cgcccagcgt cttcagatgg cagcgttcga agcgcgcggt
     6361 cggctggtcg cggacgattc cctcgagaac ctgtgggatt tcgcgcagcg tcgcggtggg
     6421 cgtatcgaag gtcacgcgga tggcggcaag gattcgctgc tccaaggaac ggccgaaatt
     6481 acgcacgcgg ctcttcagaa tatcggcgtt cgacaagatg atctgctcgc cggcgtcgct
     6541 gcgcagccgc gtggtcttga tgccgatatg ttccactttg ccgccaaagg catcgatttc
     6601 gagccggtcg cccaccacga agggcttgtc gagggcgatc gacaaggagg cgaacagatc
     6661 accgagtatg ttctgcagcg ccaaagccac cgcgacgccg ccgacgccga gaccggcgag
     6721 cagcgcggta atgttgacgc cgagattgtc gagcgccacc aggatcagga gcgaccagat
     6781 caacacgcgg gcgacgaaat tgatgacatc gagcgaccct gcgaatacct gatccgcgcc
     6841 gcgctccagc tccttggtct ccagatagaa gcgcacggcg cggcctgccc ataggccgac
     6901 ctgcaacagg acgaggatca gcacgatgtt gaagacccag cgctgcacgc gatccggcag
     6961 cgtgaggctg gtttgcgcgg cgtcgagggc gagtgcgacg aacaaaaact gcctggtatt
     7021 gccgaccagg taggcgagca gccggatcgg catgcgatgc tgcgcggccg aatatttctt
     7081 gtagcgcgat gcgaccagac gccgcacgat cgagagtacc gaccacaccg cgatggccac
     7141 gatgccggca atcagccagt cggtcaacga attgctgaga tacagctggt gggccgcatc
     7201 gattttatgc gcaagataat cgccgatcat aaaggcgatt ataggctctg cccaacgcct
     7261 aggtgacgtc ctccatccca agctctttca ttttgcgggt caacgtgttg cggccccaac
     7321 cgaggagctt ggccgcatcc tgcttcagac catccgcctt acgcaacgcc acgcggatca
     7381 agatgcgttc gacttcgggg accgcttggt ccaacaggcg ttgcgttcct gccgccaatt
     7441 cctgctccgc ccagtgcgac agcttggcca tccagtcatg gctcgcggag tgtccggccg
     7501 gtacgccgcc caagtccggc ggaatgtcct cggcgcggat ctcacgtccc ggtgcagtca
     7561 cggtcaatcg gcggcacgca ttgacgagct gtcggacatt gcccggccag tcgtagatca
     7621 ccagcgccat ctcggcgggc gaggtgagta gtttgggttc gacgccgagt tcctgtccgg
     7681 caaccgccag ataatggcgc aacagttccg gaacgtcctc gcgccgctcg cgcagcggcg
     7741 gtatctcgat gcgaatgacg ttgagtctgt gcagcagatc ttcgcgaaaa tgacctgaac
     7801 tcacccgcgc ttccaagtct tggtgggtcg cggcaatcac acgcacatcg actttgacgg
     7861 gggtctggcc gccgacccga tagaattcac cctcggcgag cacgcgcagc agccgagtct
     7921 gcagcgccgg ggacatatcg ccgatttcgt ccagaaacag cgtgccgcca tcggcctgtt
     7981 cgaagcgccc gcgacgcagc gcatcggctc cggtgaatgc gcccttttcg tggccgaaca
     8041 attcggattc caacagatcc gccgtgaagg ccgccgtgtt gagcgcaaca aaggctcggt
     8101 tcgagcgcgg gctgtgccga tgcagggcgc gcgccacgag ttccttgccg gtgcctgatt
     8161 caccggtgat caacaccgtc atgctggagc gggacagtct gcccaccgcg cgaaacacct
     8221 gctgcatggc tgccgcctgc ccgagcaatt ccgggatact gcgccgcgcg gcctccacgt
     8281 cgatggatac cgtcgcctgt tgcgcggcgc ggcgcacgag atcgaccgcc ttgtcgatgt
     8341 cgaaaggctt gggcaagtac tcgaaggcgc cgccctgata ggcagcaacc gcggcatcca
     8401 agtcggagtg cgcagtcatg acgatgaccg gcaggcccgg ccgcgacact tgaatttcct
     8461 tgagcaggtc gagtccgctg cgcccgggca tgcggatgtc ggtcatcaag acatccggcg
     8521 cgccggaacg cagggcactc aagacgttat ccgcttcatc gaagcatttg gcggtcatgc
     8581 cgctgccttt gagcgctttc tcgagcaccc agcggatgga tgcatcatcg tcgaccaacc
     8641 aaacttgcaa cggcttcgcg ctcatccaat ttctccgcga gaaacaccgg ccttcaggcc
     8701 ggcgagggat ggcgacgcgc g
//
LOCUS       DASWZT010000565         4693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_48236, whole genome shotgun sequence.
ACCESSION   DASWZT010000565 DASWZT010000000
VERSION     DASWZT010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48236"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VGE92_16170"
     CDS             <1..673
                     /locus_tag="VGE92_16170"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY1315420.1"
                     /translation="VWIRDRNIQAYIANCQESSVGSPIKPEYVAGLLTAALLSEAGDA
                     YGEGRYAEALDLYQTARKTAAGDQLRVYNGLYLSLTKLQRTAQAVAAFRDLVDFGLRR
                     QQLAVKFLFRPGSVRFASDAKFSAVYDMWLAQIAAQAAQNNICLQITGHTSPTGAAAL
                     NDSLSLLRAQYIQARLESTQPGLARRTQAAGVGSRENLIGTGKDDASDVLDRRVELQP
                     VQACG"
     gene            complement(690..2906)
                     /locus_tag="VGE92_16175"
     CDS             complement(690..2906)
                     /locus_tag="VGE92_16175"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEY1315421.1"
                     /translation="MSSASRSTSRADPIFASYVALANGLVADLSGICLLDASLKVLGQ
                     HGELSGEAVTKWIRSLGWTDSHETAPTASARASHQWWTAIPLEQSDGSLLGVFCASQH
                     IAAAPTQPSRFAAQLAGRLKPLLDCIYRDLASAIPAHSRINSLTERSAELEWLFNVTS
                     NLKGAVDDKKILQELVAQATARLESGLGVLYVPDKRLTVKCENDSAAAASLWTAWTQT
                     REQLITWAQRQNRPLVVNNVGRADSSITRCKVLCVPVVRETSRAIGALAFYNLPRAAN
                     YSNRQVFLARHIGRQAASLVEAQFDLMTGLYTRSGLDQMYLGLPESTDFVESSVIYLD
                     VDHMHVANELHGFELGNELIVRVADLLSAPLLPDDALAARISGDRFAIILPQSSCADG
                     VKIAQALQGAASRLVIGTSSEAFEVSVSCGVSALVPMPEGLARAIAAAELACKTAKNH
                     GRNRVELYAFEDGSMMRRHADALAVGQLRSALKADRLLLYAQRIAPLQNLSLPGGYEL
                     LLRLRDVDGTLVSPGPLVEAAQRYQLLPSIDRWVAQRALQMLAPYRSMFATRGLAMSI
                     NVSGQSIGDETFIQQFGQMLKSANLPRDCINVELTEQAAVTNLASAKTMVTRLAALGC
                     RFALDDFGTGANSLTYLKTLQVNRVKIDGSFVRDVLTDRNSKATVNAIVELAKGLGIE
                     TVAEHVENEAIAQEVRRLGVDYAQGYAFGRPEPLNEILDGLANDESRRLHRLFLET"
     gene            3075..3788
                     /locus_tag="VGE92_16180"
     CDS             3075..3788
                     /locus_tag="VGE92_16180"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEY1315422.1"
                     /translation="MRILAQVTDFDPKPVVITVDATTRVSGLLSTAPRAKACLVLAHS
                     AGAGMAHPFMDATARELLALGIATLRYQFPYMENRSRRPDPPALCHATVRAAAAAAHQ
                     ATPGLPLFAGGKSFGGRMTSQAQALTPLPHVRGIVFLGFPLHPPKEPSEARAKHLFEI
                     GIPMLFLQGTRDAFAEVALLQTVIARLANTASLSLFQDADHSYHVPARTGRKDEEVRG
                     DLMKTLAAWIDGVLAPSAG"
     gene            complement(3785..4450)
                     /locus_tag="VGE92_16185"
     CDS             complement(3785..4450)
                     /locus_tag="VGE92_16185"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEY1315423.1"
                     /translation="MNVAEAISERASCRAFTDQPVAIETVERILQIAMRAPSGGNLQP
                     WHVHLLAGERMREFRALMAPKLAAQPAGETSEYNVYPPQLKEPYRTRRFKCGEDLYAT
                     IGIARDNRPARLAQFAKNFNFFGAPVALFISVDRQMGAPQWADLGMFLQSVMLLLHEQ
                     GLSSCPQEAWAAWPKTVGDFVQLPPELMLFCGLAIGYRDPGAPINQLRTERAALADTV
                     TIV"
     gene            complement(4478..>4693)
                     /locus_tag="VGE92_16190"
     CDS             complement(4478..>4693)
                     /locus_tag="VGE92_16190"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY1315424.1"
                     /translation="AIGSGLLTTAGKLLFAADAGESLVAFDPEHGTPLWHARLHGVSN
                     APETYMLDDHQYVVVAAGDTLYAFTLY"
BASE COUNT          864 a         1495 c         1436 g          898 t
ORIGIN      
        1 ggtgtggatt cgcgaccgca acatccaggc atacatcgcc aattgccagg agagcagcgt
       61 cggctcgccg atcaagcccg aatacgttgc cggactgctc accgccgcat tgctgagcga
      121 ggccggcgac gcctacggcg agggacgtta tgccgaggcg ctcgatctgt accaaacggc
      181 gcgtaagacc gcggcgggag accagcttcg agtctacaac ggtctgtatc tgtcgctgac
      241 caagctgcag cgcaccgcgc aggcggtagc ggcgttccgc gatttggtgg attttgggtt
      301 gcgccggcaa cagcttgccg tcaagtttct gtttcgtccc ggatccgtgc gtttcgccag
      361 cgacgcgaaa ttcagtgccg tctacgacat gtggctggcc cagatcgctg cccaagcggc
      421 gcagaataat atctgcctgc aaatcaccgg tcacaccagc ccgaccggcg cggcggcatt
      481 gaacgacagt ctgtcgctgc tgcgtgcgca gtacattcag gcgcggctgg aaagtactca
      541 accggggctt gcccggcgca cccaggcagc gggtgtcggc tcgcgtgaaa acttgattgg
      601 taccggcaaa gacgacgcga gcgacgtgct cgatcggcgg gtcgagttgc agcccgtcca
      661 ggcctgcggc tagccgcagc tgccgcgact taggtttcga ggaatagcct gtgcaggcgt
      721 cgcgactcgt cattcgccag gccgtcgaga atctcgttca agggttccgg ccggccaaac
      781 gcatatcctt gcgcatagtc gacgcccaag cgccggacct cctgagcaat cgcttcattc
      841 tccacgtgct cggcaacggt ctcgatcccc aggcccttag cgagttccac gatcgcgttc
      901 accgtcgctt tggagtttcg atcggtcaac acatcgcgga cgaaactgcc gtcgattttc
      961 acccggttga cctgcagagt cttcaagtac gtcagcgagt tcgcgcccgt accgaaatca
     1021 tcgagcgcga atcggcagcc caacgccgcg aggcgagtga ccatcgtctt cgcgctcgcc
     1081 agattagtga ccgccgcctg ctccgtgagt tcgacgttga tgcagtctcg cggcaggttg
     1141 gcgctcttga gcatctgtcc aaactgctga ataaacgtct catcgccgat ggactggcct
     1201 gatacgttga tggacatcgc caggccgcgc gttgcaaaca tgctccgata gggagcgagc
     1261 atctgcaatg cgcgctgcgc cacccagcga tcgatggagg gcaacagttg gtagcgctgc
     1321 gcagcctcga ccaatggccc gggcgacacc agcgttccgt caacgtcgcg cagccgcagc
     1381 agcagttcgt agcctccggg gaggctcaga ttttgcagcg gggcgattct ttgagcgtac
     1441 aaaagcagcc gatcggcctt gagcgccgag cgtaactggc ccacagccag tgcatcggcg
     1501 tggcgacgca tcatgctccc atcttcaaac gcatacagtt cgacgcggtt gcggccatgg
     1561 ttcttggcgg ttttgcaggc gagttccgcc gccgcgatag ctcgcgccaa cccctcgggc
     1621 atcggcacga gagcggacac tccgcagctg acggatactt cgaacgcctc gctcgaggtg
     1681 ccgatcacca gccgcgaagc ggcaccctgc agcgcctgcg ctatcttgac gccatcggcg
     1741 cagctcgatt gcggcaaaat tatggcgaat cgatctccgg aaattcgcgc ggccagtgca
     1801 tcgtccggca acagcggtgc cgacaataaa tcggcgacgc gcacgatgag ttcgttgccg
     1861 agttcgaaac cgtgcagctc gttggccacg tgcatgtgat cgacatccag ataaataacg
     1921 ctgctttcga cgaaatccgt gctctcgggc agccccaggt acatctgatc gagaccgctg
     1981 cgcgtgtaca gtcccgtcat gagatcgaat tgcgcttcga caaggctcgc ggcctgacgg
     2041 ccgatgtgac gcgcgaggaa gacttggcgg ttagagtagt tggctgcgcg cggcaaattg
     2101 tagaacgcca aggccccgat ggcgcggctg gtctcacgca cgaccggcac gcatagcacc
     2161 ttgcaacgag tgatgctgct gtccgcgcgc ccgacattat tgaccaccaa gggtcgattt
     2221 tgtcgttgtg cccacgtaat gagctgctcc cgcgtttgcg tccaggccgt ccacagcgag
     2281 gctgccgcgg cactgtcgtt ctcgcatttg acggttaggc gcttatccgg cacgtacagc
     2341 acgccgaggc cgctttcgag tcgcgcggtg gcctgcgcta ccaactcttg gaggattttc
     2401 ttatcgtcga cggccccttt gaggttgctc gtcacattga acagccactc gagttcggcc
     2461 gagcgttccg tcagggaatt gattcgggag tgcgccggaa tcgcacttgc caagtctcga
     2521 taaatgcagt cgagcagcgg cttgagccgg ccggcgagtt gcgccgcgaa ccgcgacggc
     2581 tgggtcgggg ccgcggcgat gtgctggctg gcgcaaaaca cgccgagcag actcccgtct
     2641 gactgctcca aagggatcgc ggtccaccat tgatgggagg cgcgcgcgct cgcggtcggt
     2701 gccgtttcgt gcgagtcggt ccatcccaat gagcgaatcc acttcgtgac cgcctcgccc
     2761 gacaactcgc cgtgttgccc caacaccttg agcgatgcat cgagcaagca gatcccgctg
     2821 agatctgcca ccaggccatt ggccagtgca acatacgacg cgaatatcgg atcggcgcgc
     2881 gatgtcgaac gtgacgcgct gctcatttct aatcgctctc cctttggccg tggtggcttt
     2941 agcgcaaggc tatgctgcaa tgcggcgctc gaaccgccgg gccgctcctg gttatcggcg
     3001 gtaatgccgg taactggacc cggtcagccg cgctcctcgc gcttaccgcg acgcgcttca
     3061 cggccgctct cttgatgcgc atactcgccc aggtgacaga cttcgaccca aaaccggtgg
     3121 tgatcacggt cgacgcaacc acccgggtgt ccggattgtt atcgacagca ccgcgggcca
     3181 aggcatgctt ggtgctcgcg cacagcgcgg gggccggcat ggctcatccg ttcatggacg
     3241 ccacggcccg cgagcttctg gccttgggaa tcgccacgct gcgctatcaa tttccgtaca
     3301 tggagaatcg cagccgccgt cctgatccgc ccgcgctgtg tcacgcgacg gtgcgtgccg
     3361 ccgccgccgc cgcgcatcag gcgacgcctg ggttgccctt gttcgccggc ggcaagtcct
     3421 ttggcggccg catgacatcg caggctcagg cgctgacccc gctgcctcat gtccgcggca
     3481 tcgtttttct tggatttccg ctgcatccgc ccaaggagcc ttccgaggcg cgcgccaagc
     3541 atttattcga gataggcatt ccaatgctct tcttgcaggg cacgcgcgac gcattcgccg
     3601 aggtggcgct gttgcaaacg gtgattgcgc ggctcgcaaa cactgccagc ttgagcttgt
     3661 ttcaagatgc cgaccattcg taccatgtac ccgctcgcac cggccgcaaa gacgaggagg
     3721 tgcggggcga tttgatgaag acgctggccg cctggattga cggcgtcctg gcgccgtccg
     3781 cgggctaaac gatggtcacg gtgtccgcga gcgcggcacg ttccgtgcga agttggttga
     3841 tgggggcccc gggatcgcga tagccgatgg ccaagccgca aaacagcatc agttccggcg
     3901 gcagttggac gaaatcgccg acggtcttgg gccacgcagc ccaggcctcc tgtgggcagg
     3961 aactcaagcc ttgctcgtgc agcagcagca tgacgctttg caggaacatg ccaaggtcgg
     4021 cccattgggg agcgcccatt tgccggtcga ccgagatgaa tagcgccacg ggggcaccga
     4081 agaaattgaa attctttgcg aactgcgcca agcgcgcggg ccggttgtct cgcgcgattc
     4141 caatggtcgc gtacaggtcc tcgccgcact tgaagcgacg cgtacggtaa ggctccttca
     4201 gctgcggcgg atacacattg tattcgctcg tctcaccggc tggctgagca gctaatttgg
     4261 gtgccatgag agccctgaac tctcgcatgc gctctcccgc gagcaggtgc acgtgccaag
     4321 gctgcaagtt gccgccggaa ggcgcgcgca tcgcgatctg caaaatccgc tcaacggtct
     4381 cgatcgccac cggctgatcg gtaaatgcgc ggcaactcgc ccgctcgcta atagcctcgg
     4441 caacattcat gagttcaatc ctgatttcga gtcccaatca atacagcgtg aatgcgtaca
     4501 gcgtatctcc ggccgccacc acgacgtatt gatgatcatc cagcatgtag gtctccgggg
     4561 cattcgacac gccgtgcagc cgagcgtgcc atagcggcgt gccatgctcc ggatcgaacg
     4621 ccaccaggct ttctccggcg tccgccgcaa acaagagctt gccggcggtc gtgagcaagc
     4681 cgctgccgat cgc
//
LOCUS       DASWZT010000566         3754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_77055, whole genome shotgun sequence.
ACCESSION   DASWZT010000566 DASWZT010000000
VERSION     DASWZT010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77055"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /gene="lysA"
                     /locus_tag="VGE92_16195"
     CDS             <1..1162
                     /gene="lysA"
                     /locus_tag="VGE92_16195"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEY1315425.1"
                     /translation="EQKGVPPAANWWQEPQKRRRLLEIAARESAAFVYDTATLDDAIA
                     AVRRVSSVKRWAYAMKANWHPHLLRRIYAAGLTLECVSQGELEHAFAQVPGLDPERVL
                     FTPNFAARNEYEAAFSRGVRVTLDNLHPLMMWPEVFRGRQIFLRIDPGFGRGHHSHVR
                     TGGVHSKFGVPLDEADELVALTLRAGVRVTGLHAHTGSGVFNVDNWTQTGALLADLAV
                     RFPDVTVVDLGGGIGVPEQLGHAEIDLAALDRGVGELAAKFPHIEFWMEPGRFLVAKA
                     GVLVAVVTQLKGKGDVHYVGIATGMNSLIRPALYGAHHDIRNLTRLEEPMSHRVNVVG
                     PICESADQLGADRWLPQTREGDVLLIANCGAYGFAMASNYNLRPPAAEFIVE"
     gene            1159..2283
                     /gene="asd"
                     /locus_tag="VGE92_16200"
     CDS             1159..2283
                     /gene="asd"
                     /locus_tag="VGE92_16200"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004641000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY1315426.1"
                     /translation="MKQQVGIIGWRGMVGSVLLERMRAEQDFDVVAPTFFSTSQSGAP
                     PPLAGAPAVQDANDIAALAKLPMLISAQGGDYTQAIHPRLRAAGWQGYWIDAASSLRM
                     QKNAVIVLDPVNLPVMQAAARQGLKDFIGGNCTVSLMLMAVAGLLREGLVEWITAMTY
                     QSASGGGAQHMRELLEQMGALHRSAAPLLRNPASNILDIDRAVGTALASAEFPREHFG
                     VALAASLIPWIDKDLGTGQSREEWKAGAEANKIMGTEAHPVPVEGVCVRVGAMRCHSQ
                     ALTIKLTRDLPLPEIERLLAAANPWVRVVPNDRESSIRELSPAAVSGQMTVPIGRLRK
                     LAMGGEYLSAFTVGDQLLWGAAEPLRRTMRFLLPHVTAAV"
     gene            complement(2243..>3754)
                     /locus_tag="VGE92_16205"
     CDS             complement(2243..>3754)
                     /locus_tag="VGE92_16205"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEY1315427.1"
                     /translation="HPAVAIAELMRLLVDDALLPWAEAWRVTCRTFAYTNHTLLPEAL
                     ERWSLEVFGRVLPRHLQIIQEINAQFLDEVRIRFLGDEARIARLSLIDESGERYVRMA
                     HLACVGSHAINGVAELHSELLKRDVLKDFYELWPQKFSNKTNGVTPRRWMVLSNPELS
                     DLICEHIGTGWIKDLAQLKALEPLAENAEFRGRWRAIKQHNKQRLAALAAQRTGIAID
                     PDSMFDVLVKRIHEYKRQHLQVLHIVSLYHAIKSNPQLEVQPRTFIFGGKAAPGYHLA
                     KLMIKLITSVGDVVNRDPDVRHRLKVVFLPNFNVTNGQRVYPAADLSEQISTAGKEAS
                     GTGNMKFSMNGALTIGTLDGANIEIREEVGADNFYLFGLSAAEVYTLKTQGYRPMDFY
                     NSNAGLRDVIDLIRSGFFSRGDSELFRPLIDGLLDLDPYLLLADFQSYIECQAKVSAA
                     FGDTERWTRMSILNTARSGRFSSDRTIREYCADIWRVKPVPIQLLSHAEVGIA"
BASE COUNT          682 a         1111 c         1244 g          717 t
ORIGIN      
        1 ggaacaaaag ggcgtgccgc ccgccgccaa ctggtggcaa gagccgcaga agcggcgtcg
       61 cttgctcgag atcgcggcgc gcgaatcggc tgctttcgtc tacgacacgg ccaccttgga
      121 tgacgccatt gccgctgtac ggcgggtgag ctcggtgaag cgctgggcct acgccatgaa
      181 agccaattgg catccgcact tattgcggcg catatatgcc gctggcttga ccttggaatg
      241 cgtttcccaa ggcgagctcg agcatgcgtt cgcacaggta ccgggtctcg acccggaacg
      301 cgtgctgttc actcccaact tcgctgcgcg caacgaatac gaagcggcgt tctcgcgagg
      361 ggtgcgcgtc acgttggata acctgcaccc gctcatgatg tggccggagg tgtttcgcgg
      421 caggcagatc ttcttgcgta tcgacccggg ttttggccgc ggccatcaca gccacgtacg
      481 caccggcggc gtgcactcga aattcggtgt gcccctcgat gaggccgacg agttggtcgc
      541 cttgacgctg cgcgccggcg tgcgcgtgac cgggctgcat gcgcataccg gcagcggcgt
      601 cttcaatgtc gacaattgga cgcaaaccgg agcgctgctg gcggatctgg cggtgcgttt
      661 ccccgacgta acggtggtcg acttgggcgg cggcatcggc gtgccggaac aactgggcca
      721 cgccgaaatc gacttggctg cgctcgaccg cggtgtcggc gaactggccg caaaatttcc
      781 gcatatcgag ttttggatgg aaccgggccg gtttttggtg gccaaggcgg gcgtgctggt
      841 ggccgtggtg acgcaactga agggcaaggg cgatgtccac tatgtgggca tcgcaaccgg
      901 catgaattct ctgattcggc ctgccctgta cggggcgcac catgacattc ggaatctgac
      961 ccggctcgag gagccgatga gccatcgggt gaacgtggtc ggcccaatct gcgaatcggc
     1021 agaccagctc ggcgccgatc gctggctgcc gcagactcga gaaggagacg tacttctcat
     1081 cgcaaactgc ggagcctacg gttttgccat ggcctcgaac tataatctac gtccccccgc
     1141 agctgagttt attgtcgaat gaagcaacaa gtcggaatca tcggttggcg cggcatggtg
     1201 ggctcggtgc tcctcgagcg gatgcgcgcg gagcaggact tcgatgtcgt agcgccgact
     1261 ttcttcagca cgtcgcagtc cggtgcgccg ccgccgcttg ccggtgcgcc ggcggtgcag
     1321 gatgccaacg atatcgcagc cctcgccaag ctgccgatgc tgatcagcgc gcagggcggc
     1381 gactacactc aagcgattca tccgcgcttg cgggcggcag ggtggcaggg ctattggatc
     1441 gacgccgcgt cgagcctgcg catgcagaag aacgccgtca tcgtgctgga tccggtgaac
     1501 ttgccggtga tgcaggcggc ggcccgtcag gggctgaagg acttcatcgg cggcaactgc
     1561 acggtatcgc tgatgctgat ggcggtggcc ggattgctgc gcgagggcct cgtcgaatgg
     1621 atcaccgcca tgacctacca atcggcgtcc ggcggcggcg cgcagcacat gcgcgaactc
     1681 ttggagcaga tgggtgcatt gcatcgctcg gccgcgccgc ttctgcgaaa tcccgcgtcg
     1741 aacatactcg atatcgaccg cgccgtcggc acggcgctcg ccagcgcgga gtttccgcgc
     1801 gagcacttcg gcgtcgcgct cgcggcgagc ctgataccgt ggatcgacaa ggatttgggc
     1861 accggtcaga gccgcgagga atggaaggcg ggtgccgagg cgaacaagat catgggcaca
     1921 gaggcgcacc cggtaccggt ggagggtgtt tgcgtgcgcg tcggcgccat gcgttgccat
     1981 tcgcaagcgc tgacgatcaa gctgacccgc gacctgccgc tgccggaaat cgagcggctg
     2041 ctggccgcgg caaatccttg ggtgcgcgtg gtgccgaatg atcgcgagtc ttccatccgc
     2101 gagctgtcgc cggccgcggt cagcggccaa atgaccgtcc cgatcggccg cctgcgcaag
     2161 ctcgcgatgg gcggcgaata tctatcggcg ttcaccgtgg gagatcaatt gttgtggggc
     2221 gcggcggaac cgctgcgcag aactatgcga ttcctacttc cgcatgtgac agcagctgta
     2281 taggcaccgg cttgacgcgc cagatatcgg cgcaatattc gcgaatggtg cggtcggatg
     2341 agaatcttcc gctgcgcgcg gtgttgagga tcgacatccg cgtccagcgc tcggtatcgc
     2401 cgaaagccgc cgagactttc gcctgacatt cgatgtagga ctggaagtcc gccagcagca
     2461 ggtacgggtc gagatcgagc aggccgtcga tcagcgggcg gaacagttcg ctgtcgccgc
     2521 gtgagaagaa gccgctgcga atcagatcga tgacgtcgcg caagccggcg ttggaattgt
     2581 agaaatccat cggccggtag ccctgggttt tcagggtgta gacttcggcc gcgctcaaac
     2641 caaacagata gaaattgtcc gcgccgacct cttcgcgaat ctcgatgttg gcgccgtcca
     2701 gggttccgat ggtgagcgcg ccgttcatcg agaatttcat gttgccggtc ccggacgcct
     2761 ccttgccggc ggtcgaaatc tgctccgata aatccgccgc cgggtatacg cgctgaccgt
     2821 tggtcacgtt gaaattgggc agaaacacca ccttcaggcg atgccgcacg tcaggatcgc
     2881 gattgacgac gtcgccgacc gaggtgatca gtttgatcat gagtttcgcg agatggtatc
     2941 cgggcgcggc cttgccgccg aaaataaagg tacgcggttg cacctcgagc tgcggattcg
     3001 atttgatggc gtgatacagc gagacgatgt gcagaacctg caggtgctgg cgtttgtatt
     3061 cgtgaatgcg cttcaccagc acgtcgaaca tcgagtcggg atcgatcgcg atgccggtgc
     3121 gctgcgccgc gagggcggca aggcgttgtt tgttgtgttg cttgatggcg cgccagcggc
     3181 cgcgaaactc ggcgttctcc gccaggggct ccaatgcctt gagctgcgcc agatccttga
     3241 tccaacccgt gccgatgtgc tcgcaaatca agtccgagag ctcgggattc gacagcacca
     3301 tccagcgccg tggcgtcacg ccgttggtct tattgctgaa tttctgcggc cataattcat
     3361 agaagtcctt gagcacgtcg cgcttcagga gttcggaatg gagctcggcg acgccgttga
     3421 tggcgtggct gccgacgcag gcgagatgcg ccatgcgcac gtaacgttcg ccgctctcgt
     3481 cgatcaacga cagccgggcg atgcgtgctt catcgcccag gaagcgaata cgaacctcgt
     3541 cgaggaattg cgcgttgatt tcttggatga tctgcagatg ccgcggcaat actcggccga
     3601 atacttcgag cgaccagcgc tccagggcct cgggcaacaa tgtgtgattg gtgtacgcaa
     3661 acgtgcggca cgtgacccgc caggcctcgg cccatggcaa caaggcgtcg tcgaccagca
     3721 ggcgcatcag ttccgcaatg gcgacggcag ggtg
//
LOCUS       DASWZT010000567         6847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_82192, whole genome shotgun sequence.
ACCESSION   DASWZT010000567 DASWZT010000000
VERSION     DASWZT010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6847
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82192"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2348)
                     /locus_tag="VGE92_16210"
     CDS             complement(<1..2348)
                     /locus_tag="VGE92_16210"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1315428.1"
                     /translation="MKSHARVVVIGGGVVGASVLYHLTKAGWKDVLLIERAELTSGST
                     WHAAGGMHTVNGDPNVAKLQQYTIELYKEIERISGQSCGVHITGGIMLAGTRERLDWL
                     KMAKARGRYLGMELEIISVDEAAKLFPLLEKKHFAGAMFDPIEGHVDPYGVTHAYAKS
                     AQIGGAEIVRHTRVTDLKARSDGTWDVFTDHGNVRAEHVVNAAGLWAREVGRMVGLEL
                     PVLAMEHQYLITGDMPELVGKKEQLHAIDFEGEIYIRQERGGMLMGTYERAGVPWSAL
                     TTPWDFGQDLLPNDLERIAPSLEVGFAHFPALGAAGIKKVVNGPFTFAPDGNPLIGPI
                     RGLKNFWVACGVMAGFSQGGGVGLALSRWMVDGDPGADIWGMDVARYGDWTTLAYTNA
                     KVRENYSRRFSIRFPNEELPAARPLRTTPIYERLEAQHAVFGDYCGLEHALWFAPSAA
                     QAVDHVSFRRSNAHPFVAAECEAVQQAVGLLEISNYGKFDIRGPAAAHWLSHMMANRV
                     PAVGRIALTPMLNERGKLIGDFTMCRIAEDHFFLVGTYAAETYYLRWFDRHMPASGVS
                     VRPCAMQYVGLSVAGPSSRALLQSLVRDDLSTAAFPFLSFKRMEVGMVPGYVGRVSFT
                     GELGYEIWVTTDYQRALYDLLIKAGREHGLKLCGGRALNSMRVEKSFGSWAREYRPIY
                     GPFEAGLERFVDLKKTEFIGRSAAAEEKAAGGALKLLTFKVQAGDADAIGDEPIWHDG
                     KVVGWVTSGAFGHRVNHSLALGYVPAALAAADAGFEIEIIGER"
     gene            complement(2392..3351)
                     /locus_tag="VGE92_16215"
     CDS             complement(2392..3351)
                     /locus_tag="VGE92_16215"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY1315429.1"
                     /translation="MANTSIDRRWLPLNALRAFEGVAKHSSFTAAANALLISQSALSR
                     HVIALEKLIGTQLFERKPHALVLTKAGQHLLPAVMKSFDRLEFALDDIRNDAATSLRT
                     LRVQMPPSFAVHLAVPILRDFRRAAAEVQIDLVSPAGVGPPPSDVDVAVVYSKPTVTD
                     LVTDLLWPVRLSLVCHPNVAARHAGKSLVEFIEQNELVHVRISDQPRHYLWTQFARHA
                     NLGAVNVERGLLFDTVVLAVQYVLSGEGVALVDDTLFADYLRTEQLVRPFDATLEDGY
                     GYYLITHPEALSDTAIALFRSWLIERFGTTTAQANLHLAVSNE"
     gene            complement(3355..4674)
                     /locus_tag="VGE92_16220"
     CDS             complement(3355..4674)
                     /locus_tag="VGE92_16220"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1315430.1"
                     /translation="MSSTVLDLSRSQAPSSIERWYVLILMCLIYAINIADRYVVSTVL
                     EPIRLDLHLDDAGVAMLTSWPLALFYVTCGIPISWLADRSNRRNILSISLVVWSGFTA
                     LCGLSRGAWQFALARIGAGIGEAGGTPPSTAIVSDCFAPDRRPMAMTVLALGAPIGAW
                     IAADVAGAVARAYDWRHAFLALGVPGLVVGILVYLTVREPPRGRLDALMEDLKPSVKE
                     TWRFFRQQKAAYHVVMGGGVCALWGWGLMYWTPPFLMRTYNLDVGQAGAVTGNIHLIG
                     GSLATVLTAWLLSRPSMADPRRVVWLMAGVVALTTIPSIVAYWTHTLTVTRIMFWIFI
                     PAIYFYIGPCFGLLNNLSPCHMRSMFIAVSLLVANVFNLIVAPKLVGELSDWFAHGHA
                     TDAGSLRSAMLILAPTGFWAAYHLYMASKTIVADQKRAIAYTRAWSP"
     gene            4795..6399
                     /locus_tag="VGE92_16225"
     CDS             4795..6399
                     /locus_tag="VGE92_16225"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY1315431.1"
                     /translation="MESFDYIIVGAGTAGCVLADRLSAHGRDRVLVLEAGGTDRRFWI
                     KTPIGYGRTFAAAAVNWKYQTQPNPGLNGRSIYWPRGRVLGGSSSINALVYCRGMPAD
                     FDDWRDLGNAGWGWEHVRPYFERSERRVDAAGNVSGEGPLDVKDVTPFLHPTGRYWSC
                     AASELGIAMTDDFNGAHPEGFGSYQVTIRDGFRRSAADAFLRPALRRANVQLMTHAWV
                     GKVRFEQRRAVGVDYVRDGTARFAAANREVILCGGAVNSPQLMQLSGIGPAASLAALG
                     ITPLFDNPAVGGNLQDHLAVVYSFKATERTLNDELHSTSSKLLAGLRYLITRRGPLSL
                     SVNQFGGFVRARPDGARPDVQLYFNPVTYGTGDATRTRIEVDAFSGFYLCFQPTRPTS
                     VGRIDIVSPDFRRAPQIAPNYLSTDKDVADVVHGGQLLQAIARTRAFKTLIREPLSPD
                     LGAMGPAELVADFRARASTVYHPVGTCRMGRGAGGAVVDTALKVHGVERLRIVDASVF
                     PTVTSANTNAPTLMVAQKAADLILKG"
     gene            6415..>6847
                     /locus_tag="VGE92_16230"
     CDS             6415..>6847
                     /locus_tag="VGE92_16230"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315432.1"
                     /translation="MQLDSVKVFLVSNPPPSFGGRYFVFLKLKTACGIEGVGEVYAAT
                     FGPRVLGKMIEDLFARRFFGRDPFQIESLWRASYGSGFTQRPDISLLGVLSGLEMACW
                     DIIGKSVEQPIYNLMGGKVNERLRSYTYLYPAEGEGDEFYHD"
BASE COUNT         1318 a         2196 c         2140 g         1193 t
ORIGIN      
        1 cgctcgccga tgatttcgat ttcgaatcct gcatcggccg cggccagtgc ggcgggcacg
       61 taaccgagcg ccagagagtg gttgacgcgg tgcccgaatg caccggacgt cacccatccc
      121 acgaccttac cgtcatgcca aatgggctca tcgccgatgg catcggcgtc gcccgcctgc
      181 actttgaaag tcagaagctt cagagcgccg cccgccgcct tctcctcggc cgccgcggag
      241 cggccgatga attcggtttt cttcaagtcc acgaaacgtt ccagaccggc ctcgaacggt
      301 ccgtaaatgg gccgatattc gcgcgcccag ctgccgaaac ttttttcgac gcgcatcgag
      361 ttcagggcac gcccgccgca gagtttgagg ccgtgctcgc gaccggcctt gatcaacaga
      421 tcgtagagcg cacgttgata atcggtagtc acccagatct cgtagcccag ctcgccggtg
      481 aaggacactc ttccgacgta gccgggcacc atgccgactt ccatgcgctt gaatgacagg
      541 aagggaaatg ccgccgtcga caaatcgtcc cgcaccaagc tctgcagcag cgcgcgcgag
      601 ctcgggcctg cgaccgaaag gccgacgtac tgcatggcgc aaggccgcac cgacacaccg
      661 gaggccggca tgtgccggtc gaaccaccgt aggtagtagg tttcggctgc atacgtgccg
      721 accagaaaga aatggtcttc ggcgatgcgg cacatggtga aatccccgat cagcttgccg
      781 cgctcgttga gcatcggcgt cagggcgatg cggccgacgg cggggacgcg gttcgccatc
      841 atgtgcgaca gccagtgcgc ggcggccggc ccgcggatgt cgaacttccc atagttggag
      901 atttccaaca gcccgaccgc ttgctgcacg gcctcgcatt cggcagccac gaaggggtgc
      961 gcattggagc ggcggaagct gacgtgatcg acggcttgcg ccgcgctcgg tgcgaaccac
     1021 aacgcatgct cgagtccgca gtagtcgccg aacacggcat gctgcgcctc gagcctctcg
     1081 tatatcggcg tggtgcgcaa cggccgggcc gccggcaatt cctcgttcgg aaatcgaatg
     1141 ctgaagcggc gcgaatagtt ctcgcgaact ttggcgttgg tgtacgcgag cgtggtccag
     1201 tcgccgtagc gcgcgacgtc catgccccaa atgtcggcgc cggggtcccc gtccaccatc
     1261 cagcgcgaca gtgccaagcc gacgccgccg ccctggctga agcctgccat cacgccgcac
     1321 gccacccaga agttcttcag gccgcggatc ggcccgatca acggattgcc atcgggcgca
     1381 aatgtgaacg gcccgttcac caccttcttg atgcctgcgg cgccgagcgc cgggaagtgc
     1441 gcgaagccca cttcgagact cggcgcaata cgctccaggt cgttcggcaa caggtcctgc
     1501 ccgaagtccc acggtgtggt tagcgccgac cagggcacgc ccgcccgctc ataggtgccc
     1561 atcagcatgc cgccgcgctc ctgacggata taaatctcgc cttcgaagtc gatggcgtgc
     1621 agctgctcct tcttgccgac caattccggc atgtcgccgg tgatcaggta ttgatgttcc
     1681 attgcgagca ccggcaattc gaggccgacc atgcggccaa cttctcgcgc ccacaaaccc
     1741 gccgcgttca ccacgtgctc ggcacgcaca ttgccgtggt cggtgaacac atcccaggtg
     1801 ccgtcgctgc gcgccttgag gtcggtgacg cgcgtgtgcc gcacgatctc ggcgccgccg
     1861 atttgcgcgg atttcgcgta cgcgtgcgtc actccgtacg gatccacgtg accctcgatc
     1921 gggtcgaaca tggcgccggc gaagtgcttc ttctcgagca gcggaaacag cttagcagcc
     1981 tcatcgaccg aaataatttc caactccatg ccgagatagc ggccgcgcgc cttcgccatc
     2041 ttgagccaat cgagccgctc gcgggtaccg gcgagcatga tgccgccggt gatgtgcacg
     2101 ccgcaggact gcccggagat ccgctcgatc tccttgtaca gctcgatcgt atattgctgc
     2161 agcttcgcga cattgggatc gccgttgacg gtgtgcatgc cgccggccgc gtgccaggtt
     2221 gaacccgagg tcagctcggc gcgctcgatc agcagcacgt ctttccagcc ggccttggtc
     2281 aggtgatata gaacgctggc gccgaccacg ccgccgccga tgaccactac tctcgcgtgg
     2341 ctcttcatac caactcccgg ttaataatca gcacaccccg ccgcgcagcg cctactcatt
     2401 cgaaaccgcg agatgcagat tggcctgggc cgtggtggtg ccgaagcgct cgatcaacca
     2461 ggagcgaaac agcgcgatgg cggtatcgct cagggcctcc ggatgcgtga tcaagtaata
     2521 gccgtagccg tcctccaagg tggcgtcgaa cggccttacc aactgctcgg tgcgcagata
     2581 atccgcgaac agggtgtcgt cgaccagcgc cacgccctcg ccgctcagca cgtattgcac
     2641 ggccagcacg accgtatcga acagcaagcc acgctcgacg ttcaccgccc cgagattcgc
     2701 gtgccgggcg aattgcgtcc acaggtagtg ccgcggctga tcggaaatcc gcacatgcac
     2761 gagctcattt tgttcgatga attccaccaa gctcttgccg gcatgccggg ctgcgacatt
     2821 cgggtggcag accaaactca agcgcaccgg ccacaacagg tcggtcacca aatcggtcac
     2881 ggtcggtttc gaatagacga ccgccacgtc gacatcgctc ggcggcggcc cgacgcccgc
     2941 ggggctcacg agatcgatct gcacctcggc ggccgcgcgc cggaagtcgc gcaaaatcgg
     3001 cacggcaaga tgcaccgcaa agctcggcgg catctgcacg cgcaaggtgc gcagcgaggt
     3061 ggcagcatcg ttgcgtatgt cgtcgagcgc gaactccagc cgatcgaagg atttcatgac
     3121 cgcgggcaac aaatgctgtc cggccttggt cagcaccagc gcatgcggct tgcgctcgaa
     3181 caactgcgtg ccgatcagct tctcgagggc aatcacgtga cgcgataagg cgctctggga
     3241 aatgagcagc gcattggccg ccgcagtgaa gctcgagtgt ttcgccacac cctcgaatgc
     3301 acgcaatgcg ttcaggggca gccagcggcg atcgatcgac gtattggcca tgagtcaagg
     3361 agaccaagca cgcgtgtagg cgatcgcccg cttctgatcg gcgacgatgg ttttggaggc
     3421 catgtacaaa tgatacgccg cccaaaaacc cgtgggcgcc aaaattagca tggcgctgcg
     3481 cagcgagccg gcatcggtgg catgtccgtg ggcgaaccaa tcgctcaatt ctccgacgag
     3541 tttcggcgcg acgatcaaat tgaagacatt ggccaccagc agcgacaccg cgatgaacat
     3601 cgagcgcatg tggcacggcg agagattgtt cagcaacccg aagcatggac cgatgtagaa
     3661 ataaatggcc gggatgaata tccaaaacat gatccgtgtg acggtgagcg tatgggtcca
     3721 gtacgcgacg atcgacggaa tcgtggtgag cgcgacgacg cccgccatca gccagaccac
     3781 acggcgcgga tccgccatgg acgggcggga cagcagccac gcggtcagca ccgtcgccaa
     3841 ggagccgccg atcaaatgaa tatttccggt cacggcgccg gcctgaccga cgtccagatt
     3901 ataggtacgc atcaggaatg gcggcgtcca gtacatcaaa ccccagcccc acaatgcgca
     3961 cacaccgccg cccatcacga cgtggtaggc ggctttctgc tgtcggaaga aacgccaggt
     4021 ttccttgacc gacggtttca gatcctccat gagtgcatcc aggcgcccgc ggggcggctc
     4081 gcgaacggtc agatacacca agatcccgac gaccaaaccg ggaacaccga gcgccagaaa
     4141 ggcgtggcgc cagtcgtacg cacgcgccac cgcacccgcc acatcggcgg ctatccatgc
     4201 gccgatcggc gcgccgaggg cgagcaccgt catcgccatc ggccggcggt ccggcgcaaa
     4261 gcagtccgaa acgattgccg tggagggcgg cgtgccgccg gcttcaccga tgccggcccc
     4321 gatgcgggcc agcgcgaact gccatgcgcc gcgcgacagg ccgcacaggg cggtaaagcc
     4381 cgaccacacg accagcgaga tcgacagtat gttgcggcgg ttggagcggt cggcgagcca
     4441 ggatatcggg atcccgcagg tgacataaaa cagcgccagc ggccagctgg tcagcatcgc
     4501 aacgccggcg tcatccaggt gcagatccag gcggatgggt tcgagcaccg tcgatacgac
     4561 atagcgatcg gcaatattga tcgcgtagat caggcacatc aggatgagga cgtaccaacg
     4621 ctcgatcgac gagggtgcct gggatcgcga taaatcgagg accgtgctgc tcatgcctat
     4681 cgcaccatac agtgttcgac cgcgtttcgc gacggcgccg cgagcgtcgc aaaatgacat
     4741 gggcgaatcg cgaaatggca tagcgcgact cgagcgaaag gctaagctcc ggacgtggag
     4801 agctttgact acatcatcgt cggtgcgggc acggcgggct gcgtcctcgc ggatcggcta
     4861 tcggcgcacg gccgcgaccg ggtactggtg ctcgaggccg gcgggacgga ccgccgattt
     4921 tggatcaaga ctcccatcgg ttacgggcga acattcgccg ctgccgcggt caactggaaa
     4981 taccagacgc agccgaatcc gggcctcaac gggcgcagca tctactggcc gcgcggccgc
     5041 gtgctcggcg gctcgagctc gatcaatgcg ctggtttact gccgcggcat gcccgcggac
     5101 ttcgacgatt ggcgcgatct gggcaatgcc ggttggggct gggagcacgt gcgtccctat
     5161 ttcgagcgct ccgagcggcg ggtcgatgcc gcgggaaacg tcagcggtga gggacctctg
     5221 gacgtcaagg acgtgacccc cttcctgcac cccacggggc gctactggtc ttgcgccgcg
     5281 tccgaacttg gcatagcgat gactgacgat ttcaacggtg cgcacccgga aggcttcggc
     5341 tcctatcaag tgaccatccg cgacgggttt cgccgctcgg cggccgatgc ctttttacgg
     5401 cccgcactgc gacgcgcaaa tgtgcagctg atgacccacg catgggtcgg taaggttcgc
     5461 ttcgagcagc ggcgcgccgt gggcgtcgac tacgtacgcg acggcacagc gcgatttgcg
     5521 gcagccaatc gcgaagtgat tctctgtggc ggagcggtca attcgccgca gctcatgcag
     5581 ctgtcgggga tcgggcctgc cgcgagcctg gcagcgcttg gcatcacccc gctgttcgat
     5641 aaccccgcgg tgggcgggaa tctgcaagat cacctggctg tcgtgtattc gttcaaggcc
     5701 acggagcgca ccttgaacga tgagctgcat tcgacgagca gcaaattgct cgcgggtttg
     5761 cgctatctca tcacacggcg cggacccttg agtttgagcg tcaatcaatt cggcggcttt
     5821 gtgcgcgccc ggcccgacgg cgcgcgtccg gatgtgcagc tgtatttcaa tccggtgacc
     5881 tatggcacgg gcgatgccac ccgcacccgc atcgaagtcg acgcgttttc cgggttctat
     5941 ttgtgttttc aaccgacccg gccgaccagc gtcggtcgca tcgatatcgt cagcccggat
     6001 tttcgccgcg cgccgcaaat cgcgcccaac tacttgtcca ccgacaagga cgtcgccgat
     6061 gtggtgcatg gcgggcagtt gctgcaagcc attgcccgca cgcgagcctt caaaactctg
     6121 atccgcgaac ccctgtcgcc ggacttgggt gccatgggcc ccgcggagct cgttgccgat
     6181 tttcgcgcgc gcgcgagtac cgtgtatcac ccggtcggca cctgccgcat gggacgaggt
     6241 gcgggcggcg ccgtggtcga caccgccctc aaagttcatg gcgtggagcg cttgcgcatc
     6301 gtggacgcgt cggtttttcc gacggtcacg tcggcgaaca ccaatgcgcc gacgctgatg
     6361 gtggcgcaaa aggccgcaga tctcatattg aagggatgaa tcgaaaggaa gtgtatgcag
     6421 ctcgacagtg tcaaagtgtt cctcgtgagc aatccgccgc cgagtttcgg cggccgctac
     6481 ttcgtgttcc tgaagctgaa gacagcatgc ggaatcgagg gcgtgggcga agtctacgcc
     6541 gccaccttcg gcccgcgcgt gctcggcaag atgatcgagg atttgtttgc gcggcgcttc
     6601 ttcgggcgcg atccattcca gatcgagtcg ttgtggcgcg cgagctatgg ctcgggattc
     6661 acgcagcgcc ccgatatctc gctgctcggt gtcttgagtg gattggagat ggcgtgctgg
     6721 gacatcatcg gcaagtcggt cgaacagccc atctataatc tcatgggcgg gaaggtgaac
     6781 gagcgtctgc gaagctacac ctatctctac ccggccgagg gcgagggcga cgagttctat
     6841 cacgacc
//
LOCUS       DASWZT010000568         1551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_28857, whole genome shotgun sequence.
ACCESSION   DASWZT010000568 DASWZT010000000
VERSION     DASWZT010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28857"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..1438)
                     /locus_tag="VGE92_16235"
                     /pseudo
     CDS             complement(229..1438)
                     /locus_tag="VGE92_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
BASE COUNT          294 a          474 c          453 g          330 t
ORIGIN      
        1 aacgcacccc ggtcgcggat ttgcatcagc tgaccgcggt gtatcgggaa attctggacc
       61 gctattttgc gtagcattac agttgccgtt tcgctttgag gtgggctcgc tgagcggcag
      121 cttggtgagc tctccgccag agtgaccatg cgatgatgcg tgcgggttgg attcgctttc
      181 gagcgagtct gatggcgatg cggcggactt cctggattga ccagcggatc aacgacgggg
      241 tggcgctgct tttgtttttg ccgtggtccg gcgtctggtt ttttttggca atggcggatt
      301 ggcgcgatgg cggatcgcgg ccatcatggc aaaggcgagc atcaccagcg agacatggcg
      361 atgccatcca tgccaggatc tgctctcgtt gtgatcgagc ccgaactcgt ttttcgcggt
      421 ctcaaaactg tcctcgatcg cccaccgatg gccttcgatc gccaccagcg tttcgatgga
      481 tgttcccgct ggacaccagg tggtgaagaa ggcgagatcg ccatcggcga tacggcgacg
      541 gatcagcagg ccgcgcgtcc acaaaccatc atttgcgctg ttgaactgct cggcctcgag
      601 atcggccaat acgagatagc accaatcatg cagccgcggt cctttggttc cggcccccgc
      661 tgacaggcgt ttccagtcgg acgcgggccg cgtccgggcg atgtcggcag ccgtgccggc
      721 gactgatcta cgcttgcccc aggatcgaaa cacatgagcg ctgctgaccc cgagcacata
      781 gcccttgcct gcgcgacgca gttgttgctc gatgtcgcca acaccataga ctgtatcacc
      841 ggcaacccac tcgaatggga cagacgccgc tatcgcgcgt gcgatcattc tcgcagcaag
      901 ctttggtttg gtcgcaaagc cggtatcggg aggaacgtag gtggcttcca ggcgagttgg
      961 atcgtcgatc cattctttcg gaagatacag cgcgcgatcg atgaacgcat gaccgtggcg
     1021 cgaaacgtag gccgcgaaga cgccgatctg gcagttcgtg atcttgcccg cggaacccgt
     1081 gtactgacgc gccaccccgc acgacgcctt tccctgcttg agaaagccgg tctcgtcgat
     1141 cacgaggacc gcgtcgtcat ccgccaaatg ttcgatgaca taatcgcgga cgatatcgca
     1201 cagggcatca gcatcccaat ccctgcgacc caggatcgcc ggccgcctcc gcgcgcatcc
     1261 agccggtctt gcgttgctca tcgccgagca gaccttccag aaacaagcct gcattcttcg
     1321 caacacgctc ttgcccgaac aacggacgga tccgcttctt gatctcccga agcgccgccg
     1381 cccacaacgc aagcgtctct tcaatcgacg ctgcctgcgt ccacgatgtt cgaatcatgg
     1441 ttgcctatgg attcagaaag ctccataaaa ggcaactgta atgctagcat tacagttgcc
     1501 gtttcgcttt gaggtgggct cgctgagcgg cagcttggtg agctctccgc c
//
LOCUS       DASWZT010000569         3497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_91685, whole genome shotgun sequence.
ACCESSION   DASWZT010000569 DASWZT010000000
VERSION     DASWZT010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91685"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1397
                     /gene="dxs"
                     /locus_tag="VGE92_16240"
     CDS             <1..1397
                     /gene="dxs"
                     /locus_tag="VGE92_16240"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEY1315433.1"
                     /translation="PTDLLIVLNDNDMSISENVGALSNYLARALSGRMYSHLRESGKK
                     VLRQMPTVWELARRSEEHLKGMVLPGTLFEEMGFNYIGPIDGHDVKALVTTLRNLQKL
                     RGPQFLHVVTRKGKGYAPAEEDPIKWHGPGPFDPASGMIFKEASTGPTYSQIFGKWLC
                     DMAERDPGIIGITPAMREGSGLVEFSKRFPERYYDVAIAEQHAVTFAAGLAAEGLKPV
                     VAIYSTFLQRAYDQLIHDVALQNLPVVFALDRAGLVGSDGATHQGSYDLTFLRCIPNM
                     IIMAPADENECRQMLFTATTLSSPSAVRYPRGTGSGVAVAAEMTALPVGKAQIRREGR
                     SGLAIFAFGTLFESARKIAERLDATVVNMRFIKPLDEELTIEVAERHRALVTIEENAV
                     MGGAGSAVSEVLAAAGVQVPTLHLGIPDRFIEHGSRDTCLAAAGLDLAGLTASIEQWW
                     ASQRQERMRSVHSV"
     gene            1426..>3497
                     /locus_tag="VGE92_16245"
     CDS             1426..>3497
                     /locus_tag="VGE92_16245"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315434.1"
                     /translation="MTELPLLKIFLSSPGDVAEERALAEFVFRRLADEVSDVARLSFL
                     IWEHEPLFGHAGFQEQIERPSMSDLVVIILWSRLGTRLPADFAAVKGQPPPTGTEFEI
                     QDALASFAERGKPNLLIYRKLPGPQIALGSPDFAERSDQYARLDEFCRNTFYNAEGAV
                     VVAHHNFGDSHTFERRLAEHVRRWLDREIKVPHSERFRPFWKNESPFRGLQSFDAEHQ
                     SVYFGRAEAISDLMRRIRETEIAANTEPAPRLLLVQGMSGAGKTSLMKAGLLPLLMLR
                     PIEGVAQWLTFYLHPSESDPSMRDRGALGVLAARFCERVPAILKLGTSVRELADALFE
                     RPQEAALKIESGIIADAEIAAIEPRRIRLLVYVDQLEEAFANPEYVASEAPFIAALVA
                     LAKSGSIWITATIRSDFAHRLEAFPDLMQCLGRSPPYTLLPPRPDELAEIIREPARAA
                     GLSWEERNGVSLDQELLRDAAANPESLPLLEYTLAELYERRDGRLLRWSQYEGGLKGA
                     LISAADQVVEETPGDAATAFRDVMRELVGVGEDGAATRRYASLSRFAPGAARGLLDRL
                     VARRLCITTDEGRGDGPVTSLAHEALIRSWPRAQEWLQRETSLLRIRDELARDTAVWE
                     YHDRGDDWLGVAPEKLAAIREIEQAGLMPAGAATEYALRSRRRGRRNSLIRRTALAGI
                     CTLTVLAG"
BASE COUNT          638 a         1099 c         1111 g          649 t
ORIGIN      
        1 tgccgacgga tctgctcatc gtcttgaacg acaatgacat gtccatctcc gaaaacgtcg
       61 gcgcactgtc caattacctg gcacgcgctt tgtcggggcg catgtactcg catctgcgcg
      121 agagcggcaa gaaagtattg cggcagatgc ccaccgtttg ggaactcgcg cgccgctctg
      181 aagaacatct aaagggcatg gttctcccgg gtaccctgtt cgaagagatg ggatttaact
      241 acatcgggcc gatcgacggt cacgatgtca aagcgctggt caccaccctg cgcaacctcc
      301 aaaagctgcg cggaccacaa ttcctccacg tcgtgacgcg caaaggcaaa ggctacgcgc
      361 ccgccgaaga ggatccgatc aagtggcacg gtccggggcc gttcgatccc gcgagcggca
      421 tgatcttcaa ggaggcgagc accggcccga cctattcgca aatcttcggc aagtggctgt
      481 gcgacatggc ggaacgcgat ccgggcatca tcggcatcac gccggcgatg cgcgaaggat
      541 ccggtctcgt tgaattctcg aagcgttttc ccgagcgtta ctacgatgtt gcgatcgcgg
      601 agcagcacgc ggtcaccttt gccgccgggc ttgcggcgga gggcctcaaa cccgtggtgg
      661 ccatttattc tacttttcta cagcgcgcat acgatcaact gattcacgac gtcgcgctgc
      721 agaacctgcc ggtggtgttc gcgctcgacc gggccggttt ggtcggcagc gacggcgcaa
      781 cgcaccaggg cagctacgac ctgacgtttc tgcgctgcat tcccaacatg atcatcatgg
      841 cgccggcgga cgagaacgag tgccgccaaa tgctgtttac ggcgaccacg ctgtcctcgc
      901 cgtcggctgt gcgctatccg cgaggcaccg ggtcgggcgt cgccgtggcc gcagagatga
      961 ccgcactgcc ggtgggcaaa gcgcaaatca ggcgcgaagg ccgcagtgga cttgcgattt
     1021 tcgcgttcgg cacgctgttc gaatcggcgc gcaagatcgc cgagcggctc gacgcgactg
     1081 tggtcaacat gcgcttcatc aaaccgctcg acgaggaact gaccatcgaa gtcgcggaac
     1141 gtcaccgcgc gctcgtcacc atcgaagaaa acgccgtgat gggcggcgcc gggtcggcag
     1201 tgagcgaagt gctagccgcg gccggagtcc aagtgccgac cctgcacctc ggcatccccg
     1261 atcggttcat cgagcatggc tcgcgcgaca cctgtctcgc ggcagcgggt ctcgatctag
     1321 ccgggttgac ggccagcatc gagcagtggt gggcttcgca acgtcaggag cgcatgcgct
     1381 cggtccacag cgtgtagggg aaactgcctc actgcgattc cgttcatgac tgaattgccc
     1441 ctcctcaaga tcttcctctc atctcccggg gatgtggctg aggagcgggc actcgcggaa
     1501 ttcgtgtttc gtcgtctcgc ggatgaagta tcggacgttg cgcggctctc ctttctgatc
     1561 tgggaacacg aacctctgtt cggccatgcg ggcttccagg aacagatcga gcgtccgtcg
     1621 atgagcgatc tggtcgtgat cattctctgg tcgcgattgg gaacccggct ccccgccgac
     1681 ttcgccgcgg tcaaagggca acctcccccg acgggcaccg aattcgagat tcaagacgcg
     1741 ctcgcctcgt ttgccgagcg cggcaagccg aatctcctca tctatagaaa gctaccgggc
     1801 ccgcagattg ctctcggcag ccccgatttc gccgagcgca gcgatcagta cgcgcgtctc
     1861 gatgagttct gccgcaacac tttctataac gccgagggcg cggtagtcgt cgcccatcat
     1921 aatttcggcg acagccacac gttcgaacgg cggctcgccg agcatgtacg acgttggctc
     1981 gatcgggaga tcaaagtacc gcattcggaa aggttcaggc ctttctggaa aaacgaatcg
     2041 ccattccgcg gcctgcagtc cttcgacgca gaacatcagt cggtgtactt cggtcgagcc
     2101 gaggcgatca gcgatttgat gcggcgtatt cgggaaacgg aaatcgccgc gaacacggag
     2161 cccgcgccac ggctgctgtt ggtgcagggc atgagcggcg caggcaagac ctcgctcatg
     2221 aaggcgggcc tgctgccgct tctgatgctg cggccgatcg aaggggttgc ccaatggctc
     2281 actttctacc tgcacccgtc ggagtccgat ccttcgatgc gcgatcgcgg cgcgctcggc
     2341 gtactggccg cgcggttctg cgagcgtgtg ccggcgatcc tgaagctagg cacctcggta
     2401 cgagaactcg cggatgcgct gttcgagcga ccgcaggagg ctgcgctgaa aatcgaaagc
     2461 ggcatcatcg ccgatgctga gatcgccgct atcgagccac gccgcatacg gctgttggta
     2521 tacgtagacc aattagaaga ggcatttgca aatccggaat acgtcgccag cgaagcgcct
     2581 ttcattgccg cgctggtcgc gctggcgaaa tcgggatcga tttggatcac cgcgaccata
     2641 cgcagcgatt tcgcgcaccg gctcgaggcc ttccccgatc tcatgcagtg tctcggccgc
     2701 agcccgccgt acacgctgct gccgccgcgg ccggatgagc tcgcggaaat catccgcgaa
     2761 ccggcccgtg ccgccgggtt gtcgtgggaa gagcgcaacg gcgtgtccct ggatcaagag
     2821 ctgctgcgtg acgcagccgc caatcccgag tcgttgccgc tgctcgagta cacgcttgca
     2881 gagctctatg agcggcgcga tggtcgcctg ctgcgctggt cgcagtacga aggcggcctc
     2941 aagggcgcgc tgatctctgc ggccgatcag gtggtcgagg agactcccgg cgatgcggct
     3001 actgcgtttc gcgatgtaat gcgcgaattg gtgggggtcg gagaggatgg cgcggcgacg
     3061 cggcgttacg cttcattgag ccgctttgcg cccggcgccg cgcgcggcct gcttgaccgg
     3121 ctggtcgcgc gccgtctttg catcaccacc gatgaggggc gtggcgatgg cccggtgacc
     3181 tcgctcgccc atgaagcact gattcgctcc tggccgcggg cgcaagaatg gctgcagcgc
     3241 gagacctcgc tgctgcgcat ccgcgacgag ttggcgcgcg acaccgccgt atgggaatac
     3301 cacgatcgcg gcgacgactg gttgggagtt gcgccggaaa aactcgccgc catccgcgag
     3361 attgaacaag ccggcttgat gcccgcgggc gcggccacgg aatatgcatt gcggtcgcgg
     3421 cgccgcggca gacgcaattc cctgatccgg cgcaccgcgc tcgccggaat ttgcacgctc
     3481 accgtgcttg cgggcat
//
LOCUS       DASWZT010000570         3510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_53049, whole genome shotgun sequence.
ACCESSION   DASWZT010000570 DASWZT010000000
VERSION     DASWZT010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53049"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2223)
                     /locus_tag="VGE92_16250"
     CDS             complement(<1..2223)
                     /locus_tag="VGE92_16250"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEY1315435.1"
                     /translation="MIDARLESYSSDTRRYDELLDSSGQVRAHWRPLIDALASDGADG
                     VRRGVELARRLIIENGVTYNVYADPQGRDRPWVLDPLPVLLTAAEWREIELGVAQRAQ
                     LFDALLADMYGPQQLLADGTVPAELPFGHPNFLWPCHGIKPAGGRWLHVYAVDISRSA
                     DGRWWALDDRTQTPSGPGYALENRQIVSRVFADLLGDLGVRPLGGFFAGLRESLLQHA
                     PDAETPLAVVLTPGSFNETYFEHAYLARQLGLPLVEGHDLTVRGDTLFLKTLGGLCRV
                     HAILRRLDDDFCDPVELRADSALGVPGLIGVVRAGRVAIANGLGSGVLESAAWMGFMP
                     AAAERLLGEKLRLPSVATWWCGERLALDYVLENLERLVIKPAFPNQKLPAIFGRSLSA
                     DGRADLTRRLKAQPHAYVAQERLAFSQAPVWRSMAGSGAQGFAARALGIRVYAVATAA
                     GYRVMPGGLARIASDTADIVSMQRGGGSKDVWVLTPERGSAEEPNAAEAPPHAAARHD
                     DLPSSLAENLFWLGRYSERCEGKARLLRATLGMRPNTLLWPQMLETCRNFVAPSLFDA
                     VNPLALSADLQRLGWCAAQARSRLSAENWRAIGVLQREFQDAALAKRDPRETLDALLL
                     SLAGLAGFALDDMTQDDGWRLMMVGRRLERLQFLSELLSHRLRAGHTPAQSELEWLLD
                     IGDSTITYRTRYLAAPRLAPTIDLLVYDQSNPRALAFQWHAIEYSLVRVAASLGGSPD
                     D"
     gene            complement(2204..>3510)
                     /locus_tag="VGE92_16255"
     CDS             complement(2204..>3510)
                     /locus_tag="VGE92_16255"
                     /inference="COORDINATES: protein motif:HMM:NF021424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY1315436.1"
                     /translation="VHPAENWQQLAEGTTFLYEAARESRLSSEKFMLDGRHTGTGGGN
                     HIVLGGASAAESPFLRRPDLLRSLLCYWHNHPSLSYLFSGLFIGPTSQAPRIDEARND
                     SLYELEVAFAQFPTPGAAVPPWLIDRLLRNILIDVSGNTHRTEFCIDKLYSPDGPTGR
                     LGLLEMRAFEMPPHARMSLTQQLLLRAMVARFWRKPYAPANLVRWGTELHDRFLMPYF
                     VEQDFADVIAEMREAGFALEAEWFAPHFEFRFPKYGDFAARGTLVELRQALEPWHVMG
                     EEGSAGGAVRYVDSSLDRLQVKVSGLAPNRYWVTCNGRALPLQPTGQVGEFVAAVRYR
                     AWQPSSALHPSIGVHAPLTFDLYDTWMNRSLGGCQYHVTHPGGRSHEQFPVNSFEAES
                     RRLARFFRMGHTPGTMPASAPRSDAEFPFTLDLRLSDDRRTP"
BASE COUNT          650 a         1174 c         1137 g          549 t
ORIGIN      
        1 gtcgtccggc gagccgccga gcgatgccgc cacccgcacc aacgaatatt cgatggcatg
       61 ccactgaaac gcgagcgccc gcggattgct ttggtcgtac accagtagat cgatggtcgg
      121 agccagtcgc ggcgcggcga gataacgggt gcggtaggtg atggtgctgt cgccgatgtc
      181 gagcagccat tcgagctcgc tctgcgctgg agtgtgcccg gcgcgcagcc ggtgcgacag
      241 gagttcggac agaaactgca gacgttcgag gcggcgcccg accatcatca agcgccagcc
      301 gtcgtcctgg gtcatgtcat cgagtgcaaa cccggcgagc cccgcgagcg acagcagcag
      361 cgcatccaag gtctcgcgcg gatcgcgctt ggcgagcgcg gcatcttgaa actcgcgttg
      421 cagcactccg atggcacgcc agttctccgc cgacaagcgg ctgcgcgcct gcgcggcgca
      481 ccacccgagt cgttgcagat cggcgctcaa ggcgagcgga ttgacggcgt cgaatagcga
      541 cggcgccacg aaattacggc aggtttcgag catctgaggc cacagcagcg tattcggacg
      601 catgcctaag gtcgcgcgca acagccgcgc cttgccctcg cagcgttcgc tgtagcgccc
      661 gagccagaat agattctccg ccaggctcga cggcagatcg tcgtgacgcg cggcggcatg
      721 cggcggcgct tccgcggcat tcggctcctc ggccgatccg cgctcgggcg tcagcaccca
      781 aacatccttg ctgccgccgc cgcgctgcat ggagacgatg tctgcggtat ccgacgcgat
      841 acgggcgagt ccgcccggca tcacacggta gcccgcggcg gtggcgacgg catacacccg
      901 aatgccgagt gcgcgcgccg cgaatccctg cgcgccgctg ccggccatgc tgcgccatac
      961 cggcgcctgc gagaacgcca ggcgctcctg cgccacgtag gcgtgcggct gcgccttgag
     1021 ccgccgcgtc aaatcggcgc gcccgtcggc gctcaagctg cggccgaata tggcgggcaa
     1081 tttctggttc ggaaatgccg gcttgatgac caggcgttcg agattctcga gcacatagtc
     1141 gagcgccagc cgctcgccgc accaccatgt ggcgaccgac ggcaggcgca gcttctcgcc
     1201 cagcaatcgt tccgcggccg cgggcatgaa tcccatccat gcggccgact cgagcacgcc
     1261 gctgcccaaa ccgttggcga tcgccactct gccggcgcgc accacaccga tgagaccggg
     1321 tacgccgagc gccgagtcgg cgcgcagttc caccgggtcg cagaagtcat cgtcgagccg
     1381 ccgcaagatg gcgtgcaccc ggcacagacc gcccagcgtt ttcaggaaca gcgtatcgcc
     1441 gcgcacggtg aggtcatgac cctcgaccag cggcaatccc agttggcgcg cgaggtaggc
     1501 gtgctcgaaa tatgtctcat tgaacgagcc tggggtgagc accacggcga gcggtgtctc
     1561 ggcgtcgggc gcgtgctgca gtaagctctc gcgcagcccg gcgaagaacc cgccgagcgg
     1621 ccgcacgccc aaatcgccga gcaaatctgc aaatacgcgc gacacgatct gccggttctc
     1681 gagcgcatag cccggcccgg acggcgtctg cgttctgtcg tcgagcgccc accaacggcc
     1741 gtccgcggag cgcgagatat cgaccgcgta gacgtgcagc cagcggccgc ccgccggttt
     1801 gatgccgtgg cagggccaga gaaaattcgg atgtccgaac ggcagttcgg cgggcaccgt
     1861 gccgtcggcg agcagttgct gcggaccata catgtcggcg agcagcgcat cgaataattg
     1921 cgcccgctgc gcgacgccga gttcgatctc gcgccattcc gcggccgtca gcaggaccgg
     1981 caacggatcg agcacccatg ggcggtcccg tccttgcgga tcggcataca cgttgtaggt
     2041 gacgccgttc tcgatgatca gccggcgcgc caattcgacg ccgcggcgca cgccgtccgc
     2101 accgtcgctc gccagcgcat cgatcagcgg gcgccaatgc gcgcgcacct gcccggagct
     2161 atcgagcagc tcgtcgtagc ggcgcgtatc gctcgaatag gattcaaggc gtgcgtcgat
     2221 catcactcag gcgcaagtcg agcgtgaacg gaaattccgc gtcgctgcgc ggcgctgatg
     2281 ccggcatggt tccgggcgta tggcccattc ggaagaagcg tgccaagcgc cggctctcgg
     2341 cctcgaacga gttgaccgga aattgctcat gactgcgtcc gcccggatgc gtgacgtggt
     2401 attgacagcc gccgagcgaa cgattcatcc aagtatcgta cagatcgaaa gtgagcggcg
     2461 cgtgcacgcc gatgctcgga tgcaacgcgg acgagggttg ccatgcccgg taacgcaccg
     2521 cggcgacgaa ttctccgact tgtcccgtcg gctgcagcgg cagagcgcgg ccgttgcagg
     2581 taacccaata gcgattcggc gcaagcccgc tgaccttgac ctgtagccga tcgagcgacg
     2641 aatcgacata ccggacagcg ccgccggccg aaccttcctc gcccatcacg tgccagggct
     2701 cgagggcttg gcgcagttcc accagcgtgc cgcgcgcggc gaaatcgccg tatttcggaa
     2761 atcgaaattc gaaatgcggg gcgaaccact cggcctcgag agcaaagcct gcttcgcgca
     2821 tctctgcgat gacgtcggcg aagtcctgtt cgacgaagta cggcatcaag aaccggtcat
     2881 gcagctccgt gccccagcgg accaggttcg caggcgcgta gggcttgcgc cagaagcgcg
     2941 ccaccatcgc gcgcagcaac agctgctgcg tgagactcat gcgcgcatgc ggcggcatct
     3001 cgaacgcccg catctcgagc aaccctaagc gccccgtcgg tccatccggc gaatacagct
     3061 tgtcgataca gaactcggtt cgatgcgtgt tgcccgagac atcgatcaag atattgcgca
     3121 gcaggcggtc gatgagccac ggcggcaccg ctgcaccagg cgtcggaaat tgtgcaaacg
     3181 caacctccag ctcgtacaac gagtcgttac gcgcctcgtc gatgcgcggc gcctgcgaag
     3241 tagggccgat aaacaagccg gaaaaaaggt atgacagcga aggatggttg tgccagtagc
     3301 agagcaggct gcgcaacagg tcgggacgcc gtaggaacgg cgactcggcg gcgctcgccc
     3361 cgcccagtac gatgtgattg ccgccgccgg tgcctgtatg acgtccatcc agcatgaatt
     3421 tctcgctgct caggcgcgac tcgcgcgccg cttcgtacag gaaagtggtg ccctcggcca
     3481 actgctgcca attctcggcc ggatgtacat
//
LOCUS       DASWZT010000571         7746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_72418, whole genome shotgun sequence.
ACCESSION   DASWZT010000571 DASWZT010000000
VERSION     DASWZT010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7746
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72418"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="VGE92_16260"
     CDS             <1..1244
                     /locus_tag="VGE92_16260"
                     /inference="COORDINATES: protein motif:HMM:NF037276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315437.1"
                     /translation="NIAALADPKQATADQRPARFVRIEKAVEIPDKTVRKINASAFGP
                     AGMGMREILAYVPVEPDGSVKVQLPANVPFTIDVLDKNARRVGAQHTSWMQLIAGETK
                     TCNGCHTAGNLTTPSHGRSGLTKSVYAGAAAAGAPFPNTVASLPAANPGDTMADTRAW
                     NTCAQDLTPPGATTPCSELPTIDVSYSDVWTNPAAAGRAADAPLSYLYANLSTPSPAN
                     AHCATWDPLCRSTIHYPDATADGAVSHQIQPIWNLTRPSTVVGDVNPDHTCITCHNPV
                     SAAKAVEVPAGQLDLTDSASSVDTTVTTSYEELLFAHNEQTLNMGVLTDLLVPAPGPP
                     GPGGTPTTVMVPVSLAPPLTAGSASGSVPKFLRMFDGTYHDPVVDHTGYLTTAELRLI
                     SEWLDIGAQYYNDPFVAPVAN"
     gene            1249..1980
                     /locus_tag="VGE92_16265"
     CDS             1249..1980
                     /locus_tag="VGE92_16265"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEY1315438.1"
                     /translation="MRPLFTLFLLLQAFSGLVHAKEQLLEVVVAEPYLEMHSGPGRGY
                     PVVYVVGRDEIVTVLYSRTDWFKVRAPRGQEGWARRSELALTKLASGEPAPIPPYPDF
                     ATHRWEIGAGYGVFNHQNLVTAWGDFGLTESLDIEFVLQQALGNIDNRYITSLGMRHT
                     FIPEWRWFSPTAGIGTGYQYIDDKVPPKPLQTHNQLAYVSVGARGFITRRFMWRADWR
                     KYLIFTNQNQNEDPEEWKFGLAVFF"
     gene            1950..2621
                     /locus_tag="VGE92_16270"
     CDS             1950..2621
                     /locus_tag="VGE92_16270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HEY1315439.1"
                     /translation="MEIRLSSVLLAAALLSGCGWFHRGEKAPAQVVAEEPQAEPSIIE
                     PQVNRREVKTPKIKAKDFELGGYFGALSIQDFGTNPIYGVRAAYHVSEDIFLEGFLAR
                     SKAGTTSLEDVFPEITVVSDSGRRFTYYDLDLGYNVLPGEIFLGRGHAFNSALYVTVG
                     LGDVKFADTDQFALNFGMGERLLITDWLAMHLDVRDHVFETDLTGRTKNVHNIEATLG
                     LTVFY"
     gene            2639..3145
                     /locus_tag="VGE92_16275"
     CDS             2639..3145
                     /locus_tag="VGE92_16275"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY1315440.1"
                     /translation="MQLFTTKFLGRCMTAIVIASSAFAASGDASGPAPAFTLTALSGQ
                     QAALSQYKGQVVMVNFWATWCGPCQQEMPLLDQMYKKYKPAGFTLIGVNVDKEAPPVK
                     ELMAKKPVSFPVLLDPANQVSKAYHVDEMPSSVIIDRKGEIRYIHRGYKPGDENEYQD
                     RIRQLIRE"
     gene            3142..3372
                     /locus_tag="VGE92_16280"
     CDS             3142..3372
                     /locus_tag="VGE92_16280"
                     /inference="COORDINATES: protein motif:HMM:NF025452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HEY1315441.1"
                     /translation="MSLRAALTAVVIGSGLLQGACSIQPWVKPYERDRLADPIMSWDR
                     DAISGAYLDHIYESREGSRGATGTAGGGCGCN"
     gene            3378..4565
                     /locus_tag="VGE92_16285"
     CDS             3378..4565
                     /locus_tag="VGE92_16285"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HEY1315442.1"
                     /translation="MRTVPRHLSRLAAVAALTLMCVAGAAVLPDDRADVFWSSYRGGG
                     MDITGESVLVRKKFSEQFAVEANYFIDKVSGASIDVLSNASVIKDERKQKSLSLEYIH
                     DKTTYNLSYTNSTERDYISNTTHFSLSQDMFGDLTTISLGFTDSRNKVGENNGTAFKP
                     VISWLGYSTSRSYEGGLSQIITKNLIASTNLEVISDQGFLSNPYRSIRFFDPTVPLGY
                     SLGTQVYPNTHTSTAVETRAKYYLPYRAAASVSYRYFNDTWGVRANTIELDYIQPVSN
                     KFIFEGRVRHYSQNHATFYSDIFPQANYQNFEARDQNLAASTNNTFDGKMTWAFAPQG
                     FLIFKRATVSFDVTRIQFKYGDFRNIKFYNDTPGGGNYPAGEEPLYAFDAMVYQVYLS
                     MFF"
     gene            4580..4882
                     /locus_tag="VGE92_16290"
     CDS             4580..4882
                     /locus_tag="VGE92_16290"
                     /inference="COORDINATES: protein motif:HMM:NF013517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HEY1315443.1"
                     /translation="MNSGRSLKRRALLRILGASAAVTAALHLRAVRGAEPGKLDVKDP
                     AAIAQGYVEKSTVAGSTCENCLLLQGSAGAGYRPCSLFPGKLVSSSGWCKGWTPEV"
     gene            complement(4804..5799)
                     /locus_tag="VGE92_16295"
     CDS             complement(4804..5799)
                     /locus_tag="VGE92_16295"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEY1315444.1"
                     /translation="MNELPSKILVIAHRGASGERPEHTLASYALAIEEGADYIEPDLV
                     VTRDGVLIARHENEIGGTTDVARHPEFAARQRSQVIDGETMSGWFTEDFTLAEIKTLR
                     ARERLPQLRPQNCAFDGQFSVPTFDEILQLANEANRRRTPAARIGVYPETKHPAHFAG
                     IGLPQEQAVLDCLQRHGYGEAGSPVFIQSFDPRNLRQLQGMTRLPLLQLLEYEIGDVA
                     QIAQYASGIGIAKALASEAAVRAAHAVNLKVHVWTFRAENEFLPEDLRRGAAPAAHGD
                     LDGEIERYLRRGIDGFFVDFPAIGVRARNAYTSGVQPLHQPLDDTSLPGNRLQGR"
     gene            complement(5884..6075)
                     /locus_tag="VGE92_16300"
     CDS             complement(5884..6075)
                     /locus_tag="VGE92_16300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315445.1"
                     /translation="MYRRKVTLAAAALFAIGIAFAAQPTSEHADSGKAPLPGTLWVPG
                     TNMLMPAADAGMSTLALPR"
     gene            complement(6094..6273)
                     /locus_tag="VGE92_16305"
     CDS             complement(6094..6273)
                     /locus_tag="VGE92_16305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315446.1"
                     /translation="MKVSLVGLVIGCAAAGSAMAAMESPPYLPPQGTVMESPPYLPPQ
                     GTVMESPPYLPPQQQ"
     gene            6448..7020
                     /locus_tag="VGE92_16310"
     CDS             6448..7020
                     /locus_tag="VGE92_16310"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEY1315447.1"
                     /translation="MQVPASFTQLLTEWRSGHPQALERLTPLVYDELRRLARNYMRAE
                     RGSHTLQATAVVHEAFIRLIHANIALQDRGHFFAVASRLMRRVLVDHAKSRSRLKRNA
                     GAQDRNAAADTDNAAAPLQFDVIALDDAIEGLQRMEPRLARAIELHYFGGLTHEQIAA
                     AVGSSVATVQRDIRLARAWLLNEIGGTWPA"
     gene            7017..>7746
                     /locus_tag="VGE92_16315"
     CDS             7017..>7746
                     /locus_tag="VGE92_16315"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY1315448.1"
                     /translation="MIPQARWQQIQSLFEELIETGPTERLARLASACGDDKELRSSLE
                     SLLASDQTREDPLLQAIGEAAESLLEGHQDRLIGAQVGPYRVTSILGHGGMSTVYRGE
                     RDESQYQQTVAIKVLQHATLHPRLRSRLHSERHILATLDHPSIARLIDSGDLEDGTPY
                     LVMEYVDGESIDRYCDSRTLFVRERLELFVQVCAAVQYAHRNLVVHRDIKCANIFVTD
                     AGAPKLLDFGIAKLLAPESLSHTLP"
BASE COUNT         1498 a         2456 c         2354 g         1438 t
ORIGIN      
        1 cgaatatcgc ggccttggcc gaccccaaac aggccaccgc ggatcagcgt ccggcgcgct
       61 tcgtgcgaat cgaaaaagcg gtcgagatcc ccgacaagac cgtgcgcaag atcaacgcct
      121 cggctttcgg tccggccggc atgggcatgc gcgaaatact tgcctatgtg ccggtcgaac
      181 cggacggctc ggtgaaagtt caattgccgg cgaatgtgcc gttcacgatc gatgtactcg
      241 acaagaacgc gcggcgcgtc ggcgcacaac acaccagttg gatgcaactg atcgcgggcg
      301 agaccaagac ctgcaacggc tgccacacgg ccggcaacct caccacgcca tcgcacgggc
      361 gctcgggact caccaagtcg gtctacgccg gcgcggcggc cgcgggagcg ccgtttccga
      421 acaccgtcgc gagcctgccg gccgccaatc ccggcgacac catggcggat acgcgcgcat
      481 ggaacacctg cgcgcaagac ctgactcccc ccggggcgac cacaccgtgc tcggagctgc
      541 cgaccatcga tgtcagttac agcgatgtat ggaccaatcc ggccgcggca ggccgcgcgg
      601 cggacgcacc gctctcttat ctatatgcga acctgtcgac gccgagcccg gcgaacgcgc
      661 attgcgcaac gtgggatccg ctttgccgca gcacgattca ctatccggat gcgacggcgg
      721 acggcgcggt gtcgcaccaa atacagccga tttggaattt gacgcggccg agcacggtgg
      781 tgggcgatgt caaccccgat cacacgtgca tcacctgtca caacccggtg agtgctgcaa
      841 aagccgtgga agtccccgcc ggtcagctgg atttgaccga cagcgcatcg tcggtcgaca
      901 ccaccgtgac cacctcctac gaggaactgt tgtttgcaca taatgagcag acgcttaata
      961 tgggtgtgtt gacggatctg ttggtgccgg cgcccggacc gccgggtccg ggcggcacgc
     1021 cgacgacggt catggttccg gtatcgttgg cgccgccgct gacggccggc agcgcgagcg
     1081 gctcggtgcc gaaatttttg aggatgtttg acggaacgta tcacgaccct gtcgttgacc
     1141 acacgggcta cttgactacc gcggagttgc gtttgatctc agaatggctg gacattggcg
     1201 cgcagtacta caacgatccg ttcgtcgcgc cggttgcgaa ttgagctgat gcggccgctg
     1261 ttcacgttat tcctgttgct gcaggcgttc agtggtctgg tccatgcgaa ggaacaactg
     1321 cttgaggtcg tcgtcgccga gccgtatctc gagatgcata gcggcccggg ccgcggctac
     1381 ccggtggtgt atgtggtggg ccgcgacgag atcgtcaccg tgctctacag ccgcacggat
     1441 tggttcaagg tgcgcgcacc ccgcggtcag gagggctggg cgcgccgttc ggaattggcg
     1501 ctcaccaagc ttgcctctgg ggaaccggcg ccgataccgc cgtatccgga cttcgcgacg
     1561 catcgctggg agatcggcgc gggctacggg gtgttcaacc accagaatct ggtcaccgcc
     1621 tggggtgatt tcggccttac cgagagcttg gacatcgagt tcgtcttgca acaggcactc
     1681 ggcaacatcg acaaccgtta catcaccagc ctcgggatgc gtcacacgtt cattccggaa
     1741 tggcgctggt tctcgccgac cgccggtatc ggcaccggct accaatacat cgacgataaa
     1801 gtgccgccga agccgcttca gactcacaat caactggcct atgtgtcggt gggcgcgcgc
     1861 ggtttcatca cccggcgctt catgtggcgc gcggactggc gcaaatatct catcttcacc
     1921 aaccagaatc agaatgagga tcccgaagaa tggaaattcg gcttagcagt gttcttctag
     1981 cggcggcgct gctgtccggc tgcggctggt tccatcgcgg cgagaaagcg cccgcgcagg
     2041 tggttgccga ggagccgcag gcggagccgt cgatcatcga accgcaggtc aaccgccgcg
     2101 aagtcaaaac gccgaagatc aaggccaagg acttcgagtt gggcggttat ttcggcgcgc
     2161 tcagcattca ggatttcggc accaatccca tctacggcgt gcgcgcggcg taccacgtct
     2221 cggaagatat cttcttggag ggattcttgg cgcgctccaa ggccggcacc accagcctcg
     2281 aggacgtgtt ccccgagatc acggtggtca gcgactctgg gaggcgcttc acgtattacg
     2341 acctggacct aggctacaac gtacttccgg gcgagatctt tctcggccgc ggacacgcgt
     2401 ttaattcggc gctatacgtg acggtcggct tgggcgatgt gaagtttgcc gacacggatc
     2461 agttcgcgct caacttcggc atgggcgagc ggctgttgat tacggattgg ctcgccatgc
     2521 atttggacgt ccgcgaccac gtattcgaga ccgacttgac cggccgaact aagaacgtcc
     2581 acaacattga agccactctc ggcttaaccg ttttctactg atcaggagga cagatcaaat
     2641 gcaacttttt acgacgaaat ttctcggccg ctgcatgacc gcaatcgtca ttgcttcgtc
     2701 ggcgtttgcg gcgagcggcg atgcatcggg gcccgctccc gccttcacgc tcaccgcgct
     2761 cagcggacag caagccgccc tcagccagta caagggccag gtggtaatgg tgaatttttg
     2821 ggcgacctgg tgtggaccct gccagcagga aatgccgttg ctcgatcaga tgtacaagaa
     2881 atacaagccg gccggattca ccctgatcgg cgtcaacgtc gacaaggaag cgccgccggt
     2941 caaagagctg atggcgaaga agccggtgag ctttccggtc ctgctcgacc cggccaacca
     3001 agtaagcaag gcctatcacg tcgatgagat gccgagttcc gtcatcatcg atcgcaaggg
     3061 cgagatccgc tacatccatc gcggctacaa gcccggcgac gaaaacgagt accaggaccg
     3121 catccgccag ttgatccgcg aatgagcctg cgtgccgcgc taaccgcagt cgtgatcggt
     3181 tcggggctgc tgcagggtgc gtgcagcatc cagccctggg tcaaacccta cgagcgcgac
     3241 cgcctggcgg atccgatcat gtcctgggac cgggatgcca tttccggcgc gtatctggac
     3301 catatctacg agtcccgcga aggatcgcgg ggcgccaccg gcacggccgg cggcggctgc
     3361 ggctgcaact agaggccgtg cgcaccgttc cccgccatct cagtcgtctc gccgccgtgg
     3421 ccgccctgac gctcatgtgc gtcgccgggg cggccgtgct ccccgacgat cgggccgatg
     3481 ttttttggag cagctatcgc ggcggcggca tggacatcac cggagaatcg gtgctggtgc
     3541 gcaagaaatt ctccgagcag ttcgccgtgg aggcgaacta tttcatcgac aaggtcagcg
     3601 gcgcgtccat cgatgtgctc agcaacgcga gcgtcatcaa ggacgaacgc aagcagaaga
     3661 gcttatcgct cgagtacatc cacgataaga cgacctacaa cctgtcgtat accaacagca
     3721 ccgaacgcga ctacatctcc aacaccacgc atttctcgct gagccaggat atgttcggcg
     3781 atctgacgac catcagcctg ggtttcaccg acagccgcaa caaggtcggc gaaaacaacg
     3841 gcaccgcctt caagcccgtg atctcatggc tcggctactc gacaagccgc agctacgaag
     3901 gcggtttgtc gcagatcatc accaagaatc tgatcgcgag caccaacctc gaggtcatca
     3961 gcgaccaggg cttcttgagc aatccttacc gttcgattcg attcttcgac ccgacggtac
     4021 cgctcggtta ctcgctcggc acgcaggtgt atccgaatac gcataccagc actgcggtcg
     4081 aaacccgcgc caagtactat ttgccgtatc gagcggccgc gtcggtgtca taccgctatt
     4141 tcaacgacac ctggggcgtg cgcgccaata ccatcgagct cgactacata cagccggtga
     4201 gcaacaagtt catcttcgag ggccgtgtgc gccattacag tcagaatcac gcgaccttct
     4261 acagcgatat ctttccgcag gcgaactatc agaatttcga ggcccgcgac cagaacctcg
     4321 cggcatcgac caacaatacc ttcgacggca agatgacctg ggcgttcgct ccgcagggct
     4381 tcctcatttt caagcgcgcc acggtcagtt tcgatgtgac ccgcattcaa ttcaaatacg
     4441 gcgatttccg taacataaag ttctacaacg acacgcccgg cggcggcaat taccccgccg
     4501 gtgaggagcc gctgtacgcc ttcgacgcca tggtgtatca agtctacctg tcgatgttct
     4561 tttaagggcc ttacagacca tgaattcggg acgcagcctg aagcgccgtg cgctgctcag
     4621 gattctcggc gcgagcgccg ccgtgaccgc cgcgctgcac ttgcgggcgg tgcgcggcgc
     4681 cgagcccggc aagctcgatg tgaaggatcc ggccgcgatc gcgcagggct atgtcgagaa
     4741 atcgacggtc gcgggctcga cctgcgagaa ctgcctgctg ttgcaaggca gcgccggtgc
     4801 cggctatcgc ccctgcagcc tgttccccgg caaactcgtg tcatccagcg gctggtgcaa
     4861 gggctggacc cccgaggtat aggcgtttct ggcgcgcacg ccgatcgccg gaaaatcgac
     4921 gaaaaaacca tcgattccgc gccgcaagta gcgctcgatc tcgccatcca gatcgccatg
     4981 cgcggccggc gcggcgccgc ggcgcaaatc ctcgggaaga aactcgtttt cggcgcggaa
     5041 tgtccacacg tgcaccttga gattcaccgc gtgcgcggcg cgcaccgctg cctcgctggc
     5101 gagcgccttg gcgatgccga tgccgctggc atattgcgca atctgcgcaa catcgccgat
     5161 ttcgtactcg agcagctgca gcaagggcag ccgcgtcatc ccctgtagct gccgcagatt
     5221 tcgcggatcg aaggactgga tgaaaacggg actgcccgcc tcgccataac catggcgttg
     5281 caggcaatcg agaaccgcct gttcctgcgg caaaccgata ccggcgaaat gcgccgggtg
     5341 cttggtttcc gggtagacgc cgattcgggc ggcaggcgta cgccgccggt tggcctcgtt
     5401 cgccaattgc aggatctcat cgaaggtcgg cacgctgaat tgcccgtcga atgcgcagtt
     5461 ctgcggccgc aattgcggca ggcgctcgcg ggcgcgcaga gtcttgatct ccgcgagcgt
     5521 gaaatcctcg gtgaaccagc cgctcatggt ttcgccgtcg atgacctggg agcgctgccg
     5581 cgcggcgaac tctgggtgcc gggcaacatc cgtcgtgccc ccgatctcat tctcgtgccg
     5641 cgcgatgagc acgccgtcgc gtgtcactac caaatcgggt tcgatgtaat ccgcaccctc
     5701 ctcgatcgcc agcgcatagg acgcgagggt gtgttccggc cgctcgccgc tggccccgcg
     5761 gtgcgcaatg acgagaatct tggagggaag ttcgttcata tcccccagtt taacgtacgc
     5821 gcaacgatgc gaggctcccc tcatgcagga gcctcgactt aggtctcaca cgcgcccgtc
     5881 gacttaccgc ggcagcgcca gcgtcgacat cccggcatcg gccgctggca tcaacatatt
     5941 cgtacctgga acccagagcg tccccggcaa cggagccttg cctgaatctg cgtgctccga
     6001 tgtcggttgc gccgcgaacg cgatgccaat ggcgaacagc gcagcggccg ccagtgtcac
     6061 ttttcgtcga tacattacct tgccccttgc cggctattgt tgctgcggcg gcaggtacgg
     6121 cggcgattcc atcacggtgc cctgcggcgg caggtacggc ggcgactcca tcacggtgcc
     6181 ctgcggcggc aggtacggcg gcgactccat ggctgccata gcggatccgg cggctgcaca
     6241 gccgatgacc aaaccgacta aactgacctt catttgattc tccttgggtt gtggaacgag
     6301 cgacagacag acgacctgat agggcggtct gtatagatag aagcgacgcc gggattggaa
     6361 tcccatcaat tgcgccgcca caatgtatgc ttaagggaaa acctgaattg attgctatga
     6421 attttctgtc acggtgagcg aatgacgatg caggttccag cgagtttcac tcagctcctg
     6481 accgaatggc gttcgggcca tccgcaggcg ctcgagcgtt tgacgccgct ggtctacgac
     6541 gaactgcggc gcctggcgcg caactacatg cgcgcggaac gcggcagcca taccttgcag
     6601 gcgaccgccg tggtccatga ggccttcatc cggctgattc atgccaatat cgcgctgcag
     6661 gatcggggtc attttttcgc agtggcctcg cgcctgatgc gccgcgtgct ggtcgaccac
     6721 gccaaatcgc gctcgcgctt gaaacgcaac gccggcgccc aggatcgcaa cgctgccgcg
     6781 gacacggaca acgcagccgc gccgctgcag ttcgatgtga tcgcgctcga tgatgcgatt
     6841 gaagggctgc agcgcatgga accgcgcttg gctcgcgcca tcgaactgca ctatttcggt
     6901 ggcttgactc acgagcagat agccgcggcg gtcgggtcct cggtggcgac ggtgcagcgc
     6961 gacattcggc tcgcgcgcgc ctggctgttg aacgaaatcg gcggcacgtg gcccgcatga
     7021 tcccgcaggc acgctggcag caaattcaat cgctgttcga ggagctgatc gagaccgggc
     7081 cgaccgagcg gctggcacgt ctggcgagcg catgcggcga cgataaggaa ttgcgctcct
     7141 cgctcgaatc cttgctcgcg tccgaccaaa cccgcgagga cccgttgctg caggcgatag
     7201 gcgaagccgc agagtcgttg ttggagggcc accaggatcg attgatcggc gcgcaggtcg
     7261 gcccgtatcg cgtgacctcg attctcgggc acggcggcat gagcaccgtg tatcgcggcg
     7321 agcgcgacga gtcgcaatac cagcagacgg tggccatcaa ggttttgcag cacgcgaccc
     7381 tgcatccgcg attgcgcagc cgcctgcaca gcgaacggca catcctcgcc accctcgatc
     7441 acccctcgat tgcacgtttg atcgacagcg gcgatctcga ggatggaacg ccgtatctcg
     7501 tcatggagta cgtcgatggc gagtccatcg accgctactg cgacagccgc accctgttcg
     7561 ttcgcgagcg cctggaattg ttcgttcaag tatgcgccgc cgtgcagtac gcgcaccgca
     7621 atctggtagt gcatcgggac atcaagtgcg cgaatatttt cgtcaccgac gcgggcgcgc
     7681 cgaagttgct cgacttcggc atcgccaagc tgctggcgcc ggaaagcctg tcgcacaccc
     7741 tgccgg
//
LOCUS       DASWZT010000572         6528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_67523, whole genome shotgun sequence.
ACCESSION   DASWZT010000572 DASWZT010000000
VERSION     DASWZT010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6528
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67523"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1387
                     /locus_tag="VGE92_16320"
     CDS             <1..1387
                     /locus_tag="VGE92_16320"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY1315449.1"
                     /translation="VIERVLGSVWVPIETAGIVLVVLIFVLLEHEALRDRFIRIAGGS
                     DIRRTTLAINDAGERLSRFFVSQLAVNAGFALVIWLGLSIIGLPHPLLWGTLAGVLRF
                     VPYLGVWIAALCSVLLAAAVESGWTLAAVTLGFFVAVELLAAQLIEPRLYGHTTGLSP
                     LSVVIAAIFWSWLWGPIGLIVSTPLTLCLLVAGRHIKALAMLEIMLGDTQALTLPERL
                     YQRALSADYDEIIAGARAFLKRDSFANYCDSVLLPALHLARIDLVAGAITVDQQAMVR
                     SSMVAVIAAIGGEGRGMPRRRQYSVLDASNAGRELRRQREQLSGRWQGPLAVPPGSVM
                     VCVGLGSTADEFATELLVRIFRDQKIDARHMSLEDMTAATPPEAIEAVSMVYVVSAFP
                     SEERSRGEAAIHELRRRFPHAYIVAVFLRGMLLQSESEAETLPGADTAARSLGHALQI
                     CHEMHHQPAVT"
     gene            1387..2682
                     /locus_tag="VGE92_16325"
     CDS             1387..2682
                     /locus_tag="VGE92_16325"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1315450.1"
                     /translation="MANAARAPTVRIRWRIFSMLFGFGFLAYLQQKSITVAAERMMPE
                     LALSQFQISLIEWGFVFGYGVFQLPGGIFGQRQGARRTFLIIGITAFLATVATPLAPY
                     WLGGASLFVVLLLLQLFLGASQAAIFPVSAGVFEVWFPANRWAFVQGLQTMGLGLGAA
                     LTPPLITHLMTGIGWQQALLWTSLLAVPLLAVWAWYGRNSPREHPAVGEQELAEIGSQ
                     SAAPADASISAKQVLSILANRNVMLLAISYLCLNYSFYLLSNWVFLYLVQERKFSVLE
                     SGWLAMTPPLAAAVGAGVGGIITSALCLRVGNRWGYRLVPLIAMPIAGALLLISVNAA
                     NPYWAVAGLALCFAAVELNEGAFWGAAMTVGRGETMAVSGVMNTGGNLGGIIGIPIIG
                     YLSGEHLWHDAFVVGAGFAVASAAAWLGISVDHPAESPE"
     gene            2679..3452
                     /locus_tag="VGE92_16330"
     CDS             2679..3452
                     /locus_tag="VGE92_16330"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY1315451.1"
                     /translation="MRTIVITGAAQGVGLATASLLAQRDCRVILLDLNPVDAAVQRLC
                     GGGAQAAGMTGDVSSEAFVERVAQWIAQEFGAADGLVNNAGISLIAPAEATSAVQWQR
                     VLNVNLFAPFLLCRYLGAQMLARGQGSIVNVASVAGLAGVSHRSAYNASKHGLIGLTK
                     TLAAEWGGRGVRVNAVCPGWIKTEMDVADQGSGAYSDADIVNRVPMARFARAADVAEA
                     IAFLLNDERSGFINGVSLPVDGGWIADASWDSLRMRTRI"
     gene            3456..4703
                     /locus_tag="VGE92_16335"
     CDS             3456..4703
                     /locus_tag="VGE92_16335"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1315452.1"
                     /translation="MWLQHTSLNERRAMVAAYAGYGLDGFDLMIYTFIIPTLIALWGI
                     SNAQAGYIASATLVTSAIGGWAAGILADRYGRVRVLQLTVLWFAAFTFLSGFTHSYHQ
                     LLLTRALQGFGFGGEWSVGSVLVSETIEARNRGKAAGVVQSSWSIGWALAAFAYWAAS
                     TLLPTALGWRVLFWVGLLPAVLIVYIRRNVEEPPVYQATRARCARGLCKAGNFLDIFR
                     PPLLGNTLLATSLASGMLGAYYSVTTWLSTFLETERHLSVTGRTGYLLTLIAGSLAGY
                     LTSAWLSDAVGRRRGFMLFAICGALLVLAYTRMPVSGGMLLIGFPLGFFVLGIFSGMG
                     ACFSELLPNAVRASGQGFCYSAGRAIGAACPALIGVWSTHVRLGESIGAMTLGCYTVV
                     VISAWALPETRGRVLNVEAGASA"
     gene            4707..>6528
                     /locus_tag="VGE92_16340"
     CDS             4707..>6528
                     /locus_tag="VGE92_16340"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY1315453.1"
                     /translation="MDSRWQFWVDRGGTFTDVVGCAPDGGLHTYKLLSENPAQYTDAA
                     VEGIRRLLGLNPEEPITAERVACVKMGTTVATNALLERSGERTLLIITRGFRDALRIA
                     TQARPRLFDRHIVLPELLYERVIEAAERISAHGEIIEPLDESALMAQLRCAFADGLRS
                     CAIVFLHGYRYVAHERAAETLARQIGFTQISVSHRVSPLMKLVPRGDTTVVDAYLSPI
                     LRRYVDRVAQLMPGVALFFMQSSGGLTRANHFQGKDAILSGPAGGIVGMVRTAQAAGH
                     ARLIGFDMGGTSTDVSHFAGEYERAFDTQVGGVRVRAPMMSIHSIAAGGGSIIRFDGA
                     RLRVGPESAGARPGPASYRRGGPLTITDANVLLGRIQAQYFPWVLGLRGDEPLDTEVV
                     ARRFAELARAMSTADAPISAEQAAEGALRIAVGSMANAVKRISVMRGHDVSGYTLQCF
                     GGAAGQHACQVADALGMKRVFIHPLAGVLSAYGMGLADQSAMRQGAVERELDEAGVRD
                     ARLCVAALRVQAETELKAQGIDAAAASALISVARVHIRYQGTDTTLDCELPLNNTASE
                     AVAAIRGDFENGYRRRFAFLMPDHAIFIESASVECIAPGIG"
BASE COUNT         1017 a         1960 c         2188 g         1363 t
ORIGIN      
        1 ggtgattgaa cgcgtgctcg gatcggtttg ggtgccgata gaaaccgccg gtatcgtcct
       61 ggtcgtgttg atattcgttc tattggagca cgaagctctg cgcgatcgtt tcatccgcat
      121 cgctggcggc tccgacattc gccgcacgac gctcgcgatc aacgatgccg gagaacgcct
      181 atcgcggttc ttcgtctctc agttggcggt caatgcaggc tttgccctcg tcatctggct
      241 cggccttagc atcataggac tgccgcatcc gctgctgtgg ggaacgcttg ccggcgttct
      301 gcgcttcgtt ccctacctcg gagtatggat tgccgcgctc tgctcggtgc tgctggctgc
      361 ggcagtggag tccggttgga ccctggcggc cgttacactg ggattttttg tcgctgtcga
      421 gttgctcgcc gcgcagttga tcgaaccgcg attgtacggc cacaccaccg ggctatcgcc
      481 cttgtcggtg gtgattgccg cgatattctg gagttggctg tggggtccga tcggcttgat
      541 cgtgtcgacg ccgctgacct tgtgcctatt ggtggccgga cggcacatca aggcgctggc
      601 gatgctcgag atcatgttgg gagacaccca ggcgctcacc ctgccggagc gcctgtacca
      661 gcgtgcgctg tcggccgatt acgatgagat cattgccggc gcacgcgcct ttctgaaacg
      721 agattcgttc gctaattatt gcgactcggt gctgttgccc gccctgcacc tggcacgcat
      781 cgatctcgtg gcgggcgcga tcaccgtcga tcagcaagcc atggttcgca gctcgatggt
      841 ggccgtcatt gccgccatcg gcggggaggg ccgaggcatg ccgcgtcggc gtcagtactc
      901 ggtgctcgac gcctcgaatg caggtcgcga gctgcgccgg cagcgcgaac aactgagcgg
      961 ccgatggcag ggaccgctcg cggtgccgcc ggggtccgtg atggtgtgcg tcggtttggg
     1021 atccacagcc gatgaattcg cgactgagct cctggtgcgt atattccgcg atcaaaaaat
     1081 cgatgcgcgc cacatgtcgc tcgaggacat gacggctgcg acgcctcccg aagcgatcga
     1141 agcggtctcc atggtgtatg tcgtcagcgc gttcccgagc gaggagcgca gccgcggcga
     1201 ggcggcaatt cacgaattgc gccggcgctt cccgcacgca tatatcgtcg cggtgttcct
     1261 ccgcggcatg ctgctgcagt cggagagcga ggccgagacc ttgcccgggg ccgataccgc
     1321 ggcgcggtcg ctgggtcatg cgctgcaaat ctgtcacgaa atgcatcatc agccggcggt
     1381 gacgtgatgg ccaatgccgc gcgggccccc acggtacgca tccgctggcg gattttttcg
     1441 atgttgttcg gcttcggttt tctggcctat ctgcagcaga aaagcatcac ggttgccgcg
     1501 gagcgaatga tgccggaatt ggcattgagc caattccaga ttagtttgat cgagtgggga
     1561 tttgttttcg gctacggcgt attccagctg cccggcggca tttttggaca acgccaaggc
     1621 gcgcgtcgca ccttcctcat catcgggatt acggcgttcc tggcgacggt cgcaactccg
     1681 ctcgcgccgt attggcttgg cggcgccagc ctgttcgtcg tgctgttgct gctgcagctg
     1741 ttcctgggcg cctcccaggc cgcgatcttc ccggtgtcag ccggcgtatt cgaggtgtgg
     1801 tttccagcga accgctgggc cttcgtgcag ggactgcaga ccatgggatt gggactgggt
     1861 gccgccctga cgccgccttt gatcacgcat ctgatgaccg gcatcggatg gcaacaagca
     1921 ttgctgtgga ccagcctgtt ggcggttccc ttgctcgcgg tttgggcctg gtacgggcgc
     1981 aactcgccgc gcgaacatcc ggcggttggt gagcaggaat tggcggaaat cggctcgcag
     2041 agcgccgcac cggccgacgc ctcgatcagc gccaaacagg tgctgagcat tcttgccaat
     2101 cgcaacgtga tgctgctcgc gatctcctat ctatgtctga actattcgtt ctatttgctc
     2161 tcgaactggg tgttcttgta tctcgttcaa gagcgcaaat tctcggtact ggagagcggt
     2221 tggcttgcga tgacgccgcc gctcgccgcg gccgtgggtg ccggcgtcgg cggcataatc
     2281 accagcgcat tgtgcctgcg cgtcggcaac cgctggggtt accggctcgt gcccctgatt
     2341 gcgatgccca tcgccggcgc gttgctgttg atctccgtga acgccgcgaa tccttactgg
     2401 gcggtcgcgg gcctggcgct gtgttttgcg gcggtagaac tcaatgaggg cgccttctgg
     2461 ggcgcggcca tgacggtcgg gcggggcgag acgatggcgg tgagcggcgt catgaacacc
     2521 ggcgggaatc tcggcggcat catcggcatc cccatcattg gatatctgtc gggcgaacat
     2581 ttatggcatg acgcgtttgt ggtcggcgcg gggttcgccg tcgcgagcgc ggcggcatgg
     2641 ctcggaatct cggtggatca tcctgcggag tcccccgagt gagaaccatc gtcatcaccg
     2701 gcgcagcgca gggcgtcggg ctggcgactg cctcgctgct cgcgcaacgc gattgtcgcg
     2761 tgatcctctt ggacttgaac ccggtcgatg cggcggtaca acgattgtgc ggcggcggtg
     2821 cccaagcggc gggaatgacg ggtgatgtat cctcggaagc cttcgtcgag cgcgtggcgc
     2881 aatggatcgc gcaggagttt ggcgcggccg acggcctggt caacaatgcg ggtatcagcc
     2941 tgattgcgcc agcggaagcg accagcgccg tccaatggca acgcgttttg aacgtcaatc
     3001 tgttcgcgcc gttcttgttg tgccgttact tgggagctca gatgctggca cggggtcagg
     3061 gcagcatcgt gaacgtcgcc tcggtcgccg gcctcgcggg cgtgagtcac cgcagtgcct
     3121 acaatgcgtc gaagcacggt ttgatcggct tgaccaagac gctcgccgct gaatggggcg
     3181 gccgcggcgt gcgtgtcaac gccgtgtgtc ccgggtggat caaaaccgag atggacgtcg
     3241 cggatcaagg aagcggtgcc tattcggacg ctgatatcgt caatcgcgta cccatggcgc
     3301 gctttgcccg cgccgcggac gtggcggagg cgatcgcttt tctcctgaac gacgagcgca
     3361 gcggattcat caatggagtc agcctgccgg tggatggcgg ctggattgcc gacgccagct
     3421 gggacagctt gcgaatgcgc actcggattt gaaccgtgtg gctgcagcac accagtctga
     3481 acgaacgccg cgccatggtg gcggcctatg ccggctacgg cctcgatggc ttcgatctca
     3541 tgatctatac cttcatcatt ccgacgctga tcgcactttg gggaatcagc aatgcgcagg
     3601 cgggctacat cgccagcgcc accttggtca cgtcggccat cggcggctgg gctgccggga
     3661 tcctggccga tcgatatggc cgcgtgcgcg tgctgcaact cacggtgctc tggttcgcgg
     3721 cgttcacctt tcttagcggt ttcacccact cttatcacca actactcctc acgcgcgcgc
     3781 tgcagggatt tgggttcggc ggtgaatggt ccgtgggttc cgtcctggtc tcggaaacca
     3841 tcgaggcgcg taatcgcggc aaggcggcgg gtgtcgtgca aagcagctgg tcaattggct
     3901 gggcgttggc ggccttcgcg tattgggccg cgagtacgct gctgccgact gcactcggct
     3961 ggcgggtgtt gttttgggtt ggcctgctgc cagccgtgtt gatcgtttat attcggcgca
     4021 acgtcgaaga gccgccggtc tatcaagcca cccgcgccag gtgtgcgcgc gggctctgca
     4081 aggcgggaaa tttcctggat attttccgcc cgccgctgct cggcaatacg ctgttggcaa
     4141 cctcgctcgc gagcggcatg ctcggcgcct actattcggt gactacgtgg ctgtcgacat
     4201 tcctcgagac cgaacgtcat ctgtcggtca ccggtagaac cggctatctg ctgacgttga
     4261 ttgcgggttc gctggccggc tacttgacga gcgcctggtt gtcggatgca gtggggcggc
     4321 gccggggatt catgctattt gcaatttgtg gcgcgttact ggttctcgcc tatacgcgca
     4381 tgccggtcag cggcgggatg ttgttgatcg gctttccgtt gggcttcttc gtcctcggca
     4441 tattctccgg catgggcgcc tgcttcagcg aattgctgcc gaacgccgtg cgcgcctcgg
     4501 gtcagggatt ttgctacagc gcggggcgcg ccatcggggc cgcgtgcccg gcattgatcg
     4561 gtgtgtggag cacgcatgtg cgattgggcg aatcgatcgg cgccatgacg ctcggctgct
     4621 ataccgtggt cgtgatatcc gcatgggcgc tgccggagac gcgcggccgg gtactcaacg
     4681 tcgaggccgg tgcctcggcc tagcgcatgg actcacgttg gcagttctgg gtcgatcgcg
     4741 gcggcacctt caccgacgtg gtcggatgcg cgcccgatgg tggactgcac acgtacaagc
     4801 tattgtcgga gaacccggcg caatacaccg acgccgcggt cgagggcatt cgccggctgt
     4861 tgggacttaa ccccgaggaa ccgatcacgg ccgagcgtgt ggcttgcgtg aaaatgggca
     4921 ccaccgtcgc caccaatgcg ctactcgaac gtagcggcga gcgaaccctg ttgatcatca
     4981 cgcgcggctt tcgcgatgcg ctgcggattg cgacccaggc gcggccgcgg ttgttcgacc
     5041 ggcacatcgt tttgcccgaa ctcttgtacg agcgcgtgat cgaagcggca gaacgcatca
     5101 gcgcccacgg cgagatcatc gagccgctgg acgaatcggc actgatggcg cagctgcgct
     5161 gcgcgttcgc cgacggtttg cgttcttgcg cgattgtgtt tcttcacggc tatcgttatg
     5221 tggcgcacga gcgcgccgcc gaaactctgg cccggcaaat tggattcacg cagatctcgg
     5281 tgtcgcatcg cgtgagcccg ctgatgaagc tcgttccccg cggcgatacc accgtcgtgg
     5341 atgcctacct gtcgccaatc ctgcgccgct acgtggatcg cgtggcgcaa ctgatgcccg
     5401 gcgtagcgct tttcttcatg cagagctcgg gcggccttac tcgagcgaat catttccaag
     5461 gcaaggatgc gatcttgtcg gggccggccg gcggcattgt cggcatggtg cgcaccgcgc
     5521 aggcggccgg gcatgccaga ctcatcggct tcgacatggg cgggacctcg accgacgtca
     5581 gtcatttcgc gggcgagtat gagcgagcct tcgacacgca ggtgggcgga gtccgggtcc
     5641 gcgcgccgat gatgagcatt cacagtatcg cggccggcgg cggttccatc attcgtttcg
     5701 acggtgcgcg cctgcgggtg ggacccgaat ctgcgggagc ccggcccggg cctgcgagct
     5761 atcggcgcgg cggacccctc accatcacgg acgcaaacgt tctgctcggg aggatccagg
     5821 cgcaatactt cccgtgggtg ctcggcctgc gcggcgatga accattggat accgaggtcg
     5881 tcgcacggcg cttcgccgaa ttggcgcgag ccatgtcgac tgccgatgcc cccatcagcg
     5941 ccgaacaggc ggcggagggc gctctgcgca tcgccgtcgg cagtatggcc aacgcggtga
     6001 aacggatctc ggtgatgcgc ggccacgatg tgagcggcta caccttgcaa tgcttcggcg
     6061 gggcggcggg acaacacgcc tgtcaggtgg ctgatgcgct cgggatgaaa cgagtgttca
     6121 tccatccctt ggccggcgtg ctatccgcgt acgggatggg actcgcggac cagagtgcca
     6181 tgcggcaggg cgcagtggag cgcgagttgg acgaggcggg cgtgcgcgat gcgcgactgt
     6241 gcgtcgcggc tctgcgggta caggctgaga ctgaactcaa ggcacaaggc atcgatgcgg
     6301 cggcggcctc tgcgctcatc agcgtcgccc gcgtgcacat ccgctaccaa ggtaccgata
     6361 cgaccttgga ttgcgagctg ccgctgaaca atactgcgag cgaggccgta gccgcaatcc
     6421 gcggcgattt tgaaaatggt tatcgccgcc gcttcgcctt cttgatgccg gatcacgcaa
     6481 tattcatcga atcggcatcg gtggagtgca tagcgcccgg catcggcg
//
LOCUS       DASWZT010000573        12193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_19090, whole genome shotgun sequence.
ACCESSION   DASWZT010000573 DASWZT010000000
VERSION     DASWZT010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12193
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19090"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VGE92_16345"
     CDS             <1..250
                     /locus_tag="VGE92_16345"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEY1315454.1"
                     /translation="SKAARFRSRLLRDGVERATVARLVCPIGVAGIASKLPGAIAIAI
                     AAQLLQQMDAGVTTVALPDLKDCSGECQGCGAEHRKTP"
     gene            247..1770
                     /locus_tag="VGE92_16350"
     CDS             247..1770
                     /locus_tag="VGE92_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004729773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1315455.1"
                     /translation="MNEASPVPRLRLSGITKRYPTILANDAIDLSVDVGEIHAVMGEN
                     GAGKSTLMKIIYGVTRPDAGSIAWEGRNVSISSPAEARRLGIGMVFQHFALFETLSVA
                     ENISLALDERITPIALAPRIREVAERYGLPIDPQRLVHSMSVGERQRVEIVRCLLQSP
                     KLLIMDEPTSVLTPQAVQQLFATLRRLAGEGMSILYISHKLDEIRTLCDNATVLRAGR
                     VSGTARPREESNASLARLMVGSDLEEQPPVPREAGEVRLELQDFSMAARDPFGTALQH
                     IDLAVRGGEIVGIAGVSGNGQAELLTALSGEALGAHTGSISLCGQRVDALGAAQRRGL
                     GLGFVPEERLGRGAVPAMSLTDNALLTGERAGMVGHGVVKRNVARLAARATIKDFQVK
                     CGSELSAAERLSGGNLQKFIVGREIRLAPKVMLAAQPTWGVDVGAAQLIHHALIALRD
                     SGSAVLVISEELDELFTLCDRIAVLTAGRLSPPQPRAQLTVEAVGLLMTGTQESLHA"
     gene            1763..2845
                     /locus_tag="VGE92_16355"
     CDS             1763..2845
                     /locus_tag="VGE92_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1315456.1"
                     /translation="MLELPWRLERRAEPSNVMRVAAPLIASLAMLATGFVIFAVLGKA
                     PLNALEVFFVEPLNTLYGWGELLLKATPLALCALGLAIGFRANVWNIGAEGQLTLGAI
                     CGGGVALFCGARLGSLALPVILLAGVAGGAAWAAIPAYLRTRFNTSEILVSLMLVYIA
                     QLLLAYLLHGAWRDPEGYNFPQSKAFDDNTILPMLLDGQRATWAFPLMLVLAGLSWGF
                     SSKLFAGYRMLVAGQAPAAAAYAGFSERRNVWLALLLSGGAAGLAGICEVVGPIGRLQ
                     SDISPGYGFAAIIVAFVGRLHPLGIVLAALLMSLLYLGGESVQLGLQLPAAVTGLFQG
                     LLLFYLLAADLFVNFRLRGQRLASAV"
     gene            2842..3768
                     /locus_tag="VGE92_16360"
     CDS             2842..3768
                     /locus_tag="VGE92_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004729775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1315457.1"
                     /translation="MNVEFLTAVITGTVVAATPLIYAALGELVAERAGVLNLGVEGMM
                     LVGALAAFAVSVGTGSLVAGYIAAVCASMLLALIFAVLAVSLQTNQVATGLALTLFGI
                     GLSAFMGRKFLGLPIAGIQPLPLPWLKDLPIAGPLLFRFDAAVYGSILLYFIVDRVLT
                     RTRIGLTLRAVGESPAIAHALGQPVTQTRYLAVLFGGAASGIAGAYLSTAMTPMWVEG
                     LSAGRGWIALALVVFATWKPERVLLGAYLFGGVTILQLHAQGFGLRVPSEILSMLPYA
                     ATIIVLVIICRDPKTIRLNQPASLGRSFHPDA"
     gene            3778..4989
                     /locus_tag="VGE92_16365"
     CDS             3778..4989
                     /locus_tag="VGE92_16365"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEY1315458.1"
                     /translation="MSAYLIEFSSLLGRWVHLITGIAWIGASFYFVWLDNHLLPPTDA
                     ALAAKGVGGELWAVHGGGFYNAQKYRVAPRELPPTLHWFYWEAYATFLSGLFLLILLY
                     YGQAEIYLIDPRVAALSKPAAIAISIAFIAGGWLIYDALCRTALARHGAWLGGILGAL
                     LCVTAYALCHLFSGRGAFIEFGAMLGVIMVANVFFVIIPGQREMVRAKQEGRDPDPQA
                     GLRGKLRSVHNTYFTLPVLFTMLSNHYALTFGARHNWLVLIALSAVGALIRVYFVERH
                     KRHLRGGATSPWPAVLALLILGATAAALAPRQTPVSGESQLPAARFASAAHIIAVRCA
                     PCHSATPTEPGFSVAPKGLMLDTPQDILTHVAVIAPQVQTRAMPIGNLTGMTDPERTQ
                     LLDWIQRGAPH"
     gene            4973..5674
                     /locus_tag="VGE92_16370"
     CDS             4973..5674
                     /locus_tag="VGE92_16370"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY1315459.1"
                     /translation="MARLTERAALNGMDTAEVHARIWSAIMDQSLPPETRLVESELCE
                     IFGVGRTRLRQVLQRLAHERVVTLMRNRGAMVSRPSIREAREVFAARRVIEANVVETF
                     IKTATRKDVKRLHEHLAREIEAWRRNDRRAMLKLSGEFHLLLAEAAGNSILLGLLRDL
                     VSRSSLIIAVYQAPGAMPCPPDAHRELTETLERRERNAVKLMLAHLDHVFEDLTLEER
                     SDGGVDLKSVFGGRQ"
     gene            5671..6591
                     /gene="puuE"
                     /locus_tag="VGE92_16375"
     CDS             5671..6591
                     /gene="puuE"
                     /locus_tag="VGE92_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HEY1315460.1"
                     /translation="MTYARDLLGYGRKAPHARWPHGARIALSFVLNHEEGGENCVLDG
                     DAGSETFLSEIIGATAFPMRHMSMESLFEYGSRAGLWRVLRAFKERRLPLTVFAVARA
                     LERHPEAVAAYRELGHEIAAHGLRWISYQLMDEATERAHLAEAVAILTGLTGAAPLGW
                     YTGRDSPNTRRLIVEHGGFLYDSDSYADDLPYWTHVPKGAALTPHLVVPYALDSNDMR
                     FAALQGFNSGTQFFDYLRDSFDTLYREGDPLGLDAPKMLSIGLHARLCGRPGRIAALE
                     RFLDYVGTHERVWICRRIDIARHWAATHPP"
     gene            6588..8324
                     /gene="uraD"
                     /locus_tag="VGE92_16380"
     CDS             6588..8324
                     /gene="uraD"
                     /locus_tag="VGE92_16380"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEY1315461.1"
                     /translation="MITLTHLNRMSGDEFVASVGPLFEHSPWVAERAAALRPFASALG
                     LHRTMCDALMQASADEQLALIRAHPELAGRAALKGGLTAASTGEQKGAGLSACTAQQL
                     QRLQSLNSEYRSRFGFPFVLAVKGHTPDSVIAALAERVTHDLNEERQVALREICRIAR
                     FRVADLVDEPLGQAIMAMAEDLATLSETDDALTCSYLTPTHRATAARIRDYMLAAGLT
                     AHIDAVGNVVGRLDGDGRSHQRVLTGSHYDTVINAGKYDGRLGVVLPIAVAGWLRRSG
                     VKLAYPLEILGFAEEEGVRFKSTFLGSRAIAGRFDPTVLDSLDAAGVTLREAILAAGH
                     DVAAIPAIARNPADVACFVEVHIEQGPVLLAASEPVGVVTRIAGSLRSMVTITGMSGH
                     AGTVPMSLRHDAAAAAAEIVLAVERRCSAQPGVVGTVGKLEVPGGAVNVIPGRCDLSI
                     DIRSGEDALRDAADADIRAEIQRIATRRGVSATQVRVLDAASVACTPALQEAWAASAL
                     RVTGREAPRLASGAGHDAMMMASLTGIGMLFVRCGNGGISHHPQESMSADDADVAARV
                     LVDFLQHVQSPA"
     gene            8321..9703
                     /gene="guaD"
                     /locus_tag="VGE92_16385"
     CDS             8321..9703
                     /gene="guaD"
                     /locus_tag="VGE92_16385"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HEY1315462.1"
                     /translation="MTSLRLAVERAFLGPALHAPTHTELEYLPNALIIVDQQGVIRAV
                     LGSQTPEAAALSERHRAAGTLVTLEPGQYLLPGLIDLHVHAPQWPQLGSALDLPLEEW
                     LQSCTFPLEARFADTQFARTTYESLVAGLLANGTTTAVYFATIHLAATQALADICLSR
                     CQRALIGRVAMDDPGQCPAFYRDSSAQLAEAETRAFIEYVRALPGNEQRLILPVITPR
                     FIPSCTDALLAGLGRLARESGCHVQTHCSESDWEHAYVLARCGISDTAALERFGLLSR
                     HTILAHGNFIGAADAATIVAAGAGVAHCPLSNVYFSDAVFPLRRLLQQGMHVGLGTDI
                     SGGASPSILENARQAIIASRSLESGVDSTLARTQRRTPDSRIDATTAFWLATAGGGIA
                     LDLPIGLFRAGFQFDAIVIDTQAPDSNLKLEQSAAPGQILQKIIYHAARSNIHSVWVA
                     NRRVHARDAV"
     gene            complement(9700..10719)
                     /locus_tag="VGE92_16390"
     CDS             complement(9700..10719)
                     /locus_tag="VGE92_16390"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF006850.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEY1315463.1"
                     /translation="MSAETSIREYIRALPKAELHVHIEGTLEPELAFRLAAKHGVTLP
                     YGGVEELRRAYQFTDLQSFLDIYYAGSDVLRDAEDFYWLTRAYLEKIHPQGVVHVEIF
                     FDPQAHAARGVTIAAQLHGMWRALHEAEAEFGITSRLILCFLRHLSAADAMRTLEEAL
                     PHKSLIAAVGLDSTEAGHPPEKFTEVFARARQEGLPGVAHAGEEGPAAYIHGALDALQ
                     VFRIDHGVRCEEDEALMRRLALQRIPLTVCPLSNIKLKVFERIELHNLKRLLERGLCV
                     TVNSDDPAYFGGYLLENYLAVQQGLDLSREELATLARNSIEASLLTPAQKERWFADID
                     RQVQA"
     gene            10781..11191
                     /locus_tag="VGE92_16395"
     CDS             10781..11191
                     /locus_tag="VGE92_16395"
                     /inference="COORDINATES: protein motif:HMM:NF024307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HEY1315464.1"
                     /translation="MKSIRITAGGHTFIAVPHPEAPKTVAAFGKLLPYKQQIIHVRWS
                     GEACWIPLGDFDLKTGFENHTSHPSVGDVLFYPGGFSETEILIAYGSCAFASKVGQLA
                     GNHFLTIVEGKENLRALGVEVLWKGAKDILFEQL"
     gene            11215..11751
                     /locus_tag="VGE92_16400"
     CDS             11215..11751
                     /locus_tag="VGE92_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315465.1"
                     /translation="MVLIRNKEQCGVSFWYPFGFRPQRSGEVVRIIDWNKYRKWLPLR
                     ALLKVVLIGFGLLLFNDRIYTDDRFNLFALLVLISSIASLWAFIPRGTITVTPTTSVR
                     LLPLAGVEFAGNFLALGIMILVFVGQSDEVPVGAQGYDLAQFCTHLWPLAIFLVLFGL
                     LPIVVASAFVMRFGRSAP"
     gene            complement(11796..>12193)
                     /gene="ureG"
                     /locus_tag="VGE92_16405"
     CDS             complement(11796..>12193)
                     /gene="ureG"
                     /locus_tag="VGE92_16405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HEY1315466.1"
                     /translation="EDASINLEAVSRLSQRFPGLDLIFVESGGDNLAATFSPELSDLT
                     LYVIDVAAGDKIPRKGGPGITKSDLLVINKIDLAPMVGASLEVMERDAKKMRGERPFV
                     FTNLKSGQGLATVVDFIEREGLFTAAAGG"
BASE COUNT         1965 a         4014 c         3899 g         2315 t
ORIGIN      
        1 cagcaaggcg gcgcggtttc gctcgcggtt gctgcgcgac ggcgtcgagc gcgcgaccgt
       61 ggcgcgtctc gtttgcccca tcggcgttgc gggaattgcc agcaagctgc cgggcgccat
      121 cgccatcgcg atcgccgcgc agttgctgca gcagatggac gccggcgtga ccaccgtagc
      181 gctaccggat ctcaaggatt gcagcggcga gtgccaaggc tgcggcgcgg agcatcgcaa
      241 gaccccatga acgaggcatc acccgtaccg cgtctgcgat tgagcggcat caccaagcgc
      301 tatcccacca tcctcgccaa cgatgccatc gacctgtcgg tggacgtggg cgaaatccat
      361 gcggtcatgg gcgagaacgg cgccggcaag agcaccttga tgaaaatcat ctacggcgtg
      421 acgcggccgg atgcgggcag catcgcctgg gaaggccgca acgtcagcat ttcaagtccc
      481 gcggaagcac gccgcctcgg tatcggcatg gtgtttcagc attttgcgct gttcgagacc
      541 ttgagcgttg cggagaatat ctccctcgcc ttggacgagc gcatcactcc gatcgcgctc
      601 gcgccgcgca ttcgcgaagt ggccgagcgt tacggactgc cgatcgaccc gcagcggctg
      661 gtgcacagca tgtcggtcgg tgaacggcag cgggtggaga tcgtccgctg tctgctgcaa
      721 tcgccgaagc ttttgatcat ggacgaaccg acctcggtct tgacgccgca ggccgtgcag
      781 cagctgttcg cgacgctgcg ccggctcgcc ggcgagggca tgagtattct ctatatcagc
      841 cacaagctcg atgagattcg cacgctgtgc gacaacgcca cggtgctgcg cgccggccgg
      901 gtcagcggca ccgcgcgccc gcgagaggaa tcgaacgcga gcctggcgcg gctcatggtg
      961 gggagcgatc tcgaggagca gccgcccgtg ccgcgcgagg cgggcgaggt ccggctcgag
     1021 ttgcaggatt tctccatggc cgcgcgcgat ccgttcggca cggcgctgca gcatatcgat
     1081 cttgcggtcc gcggcggcga gatcgtcggc atcgcgggcg tctccggcaa cggccaggcc
     1141 gagttattga ccgccctgtc cggcgaggcg ctcggcgcgc acacgggctc catcagcctg
     1201 tgcggccagc gcgtcgatgc cttgggcgcc gcgcagcgac gcggactcgg cttgggcttc
     1261 gttcccgagg agcggctcgg acgcggcgcg gtcccggcca tgtcattgac tgacaatgcg
     1321 ctcctcaccg gtgagcgcgc cgggatggtg ggacacggtg tcgtcaaacg caacgtcgcg
     1381 cgcctcgcgg cgcgcgctac gatcaaagac ttccaggtca agtgtggcag cgaactgtcg
     1441 gcagccgagc ggctgtccgg cggcaacctg caaaaattca tcgtcggccg cgagattcga
     1501 ctcgcgccga aggtgatgct cgccgcgcaa ccgacctggg gcgtggacgt cggcgccgca
     1561 caattgatcc atcatgcgct cattgcgctg cgcgactcag gctccgccgt gctggtgatc
     1621 tccgaggaac tcgatgagct gttcacgttg tgcgatcgca tcgccgtgtt gacggccggc
     1681 cgcctgtcgc cgccgcaacc gcgcgcgcag ctcaccgtcg aggccgtggg cctgctcatg
     1741 acgggcacgc aggagagcct gcatgcttga gcttccgtgg cggctcgaac ggcgcgccga
     1801 accctccaac gtgatgcgcg tcgccgcccc gctgatcgcc tcgctggcaa tgctcgccac
     1861 cggcttcgtg attttcgccg tgctcggcaa ggccccgttg aacgcgctcg aggtgttctt
     1921 cgtcgagccc ttgaataccc tgtacggctg gggcgaattg ctcttgaaag cgacgcccct
     1981 ggcgttatgc gccttgggat tggcgatcgg atttcgcgcc aacgtctgga acatcggcgc
     2041 cgagggacag ctcacgctcg gcgcgatctg tggcggcggc gtcgccctat tctgcggcgc
     2101 acgcctgggc tcgttggcgc tgccggtgat cctgctggcg ggcgtcgccg gcggcgcggc
     2161 ctgggctgca atcccggcgt atttgcgcac ccgcttcaac accagcgaga tcctcgtgag
     2221 cctgatgctg gtgtacatcg cccaattgct gctcgcctac ctgctgcacg gtgcgtggcg
     2281 cgatcccgaa ggctataact tcccgcaatc caaggctttc gatgacaaca ccatcctgcc
     2341 gatgctgctc gacggtcagc gcgcgacttg ggcatttcca ctgatgctcg ttctcgcggg
     2401 cttgtcctgg ggtttctcca gcaagttgtt cgcgggctat cggatgctcg tggccggaca
     2461 agcgcccgcc gccgcggcct atgcgggctt tagcgaacgg cgcaatgtct ggctcgcgct
     2521 gctcctgagc ggcggtgccg cgggacttgc ggggatctgc gaagtggtgg gtccgattgg
     2581 acggctgcag tcggatatct ccccgggcta cggtttcgcc gccatcatcg tcgccttcgt
     2641 cgggcgcctg catccgctcg gcatcgtgct cgcggcgctg ctcatgtcgc tgctctacct
     2701 cggcggcgaa tcggtgcagc tcggtctgca gttgccggcc gccgttacgg gtctgttcca
     2761 gggattgctg ctgttctatt tattggcggc ggatttgttc gtgaatttcc gtctgcgcgg
     2821 gcagcgcctg gcgagcgcag tatgaacgtc gaatttctca ccgccgtcat taccggcacg
     2881 gtcgtcgcgg cgacgccgct catctatgcc gccctcgggg aactcgtcgc ggaacgcgcc
     2941 ggcgtattga acttgggcgt cgaaggcatg atgttggtcg gtgcattggc cgcgttcgcc
     3001 gtgagcgtcg gtaccggcag cctggtcgcg ggctacatcg ccgcggtgtg cgcctcgatg
     3061 ttgctggcat tgattttcgc cgttctggcg gtgagcctgc aaaccaacca ggtcgcgacc
     3121 ggtcttgcgc tcaccttgtt tggcatcggc ctgagtgcgt tcatgggccg caaattcctc
     3181 ggcctgccga tcgccggaat tcagccgctg ccgctgccgt ggctcaagga tctacccatc
     3241 gcaggcccgc tgctgtttcg tttcgacgcg gcggtgtacg gctcgattct gttgtacttc
     3301 atcgtcgatc gagtgctcac ccgcacgcgc atcggtctca cgctacgcgc cgtcggcgag
     3361 tctccggcaa tcgcccatgc gctcgggcag ccggtgactc aaacgcgcta tcttgcggtg
     3421 ttgttcggcg gcgccgccag cggcatcgcc ggcgcatacc tgtcgaccgc catgacaccc
     3481 atgtgggtcg agggcctgag cgccgggcgc ggctggatcg ccttggcgct ggtggtgttc
     3541 gcgacctgga aacccgagcg ggtactgctc ggcgcctact tgttcggcgg agtcaccatc
     3601 ctgcagctgc acgcgcaggg ctttgggctg cgcgtgccga gcgaaatcct ctccatgctg
     3661 ccctatgcgg ccaccatcat cgtgttggtc atcatctgcc gcgatccgaa gaccatccgc
     3721 ttgaatcagc cggcctcctt gggcaggagt tttcatcccg atgcgtagct cgccgtcatg
     3781 agcgcgtacc tgatcgaatt ctcgagcctg ctcggccgtt gggtgcatct gatcaccggc
     3841 atcgcctgga tcggtgcctc gttttatttt gtgtggctcg acaatcatct gctgccgccg
     3901 acggatgctg cgctcgccgc gaagggcgtc ggcggcgaac tctgggcggt gcacggcggc
     3961 ggcttctaca atgcccagaa ataccgggtt gcaccgcggg aattaccgcc gacgctgcat
     4021 tggttctact gggaagccta tgccacgttc ctgtccggtc tttttctgct gattctcttg
     4081 tattacggcc aggcggagat ttatctcatc gatccgcggg tcgccgccct gagcaagccc
     4141 gcggccatcg ccatcagcat cgccttcatc gccggcggct ggctcatcta cgatgcactg
     4201 tgccgcaccg ccctggccag acacggggcg tggctcggcg gcatcttggg cgcgctgctg
     4261 tgcgtgacgg cctacgcact ctgccatctg ttcagcggtc gcggcgcctt catcgagttc
     4321 ggcgccatgc tcggcgtcat catggtggcg aacgtgttct tcgtcatcat tcccggacag
     4381 cgcgagatgg tgcgcgccaa gcaggagggc cgcgatccgg atccgcaggc gggactgcgc
     4441 ggcaaactgc gctcggtgca caacacgtac tttacgttgc cggtgctctt tacgatgttg
     4501 agcaaccact acgcgctgac cttcggcgcg cgccacaact ggctggtgct gatcgccctc
     4561 tcggcggtgg gggcgttgat ccgcgtctac ttcgtcgagc gccacaagcg gcacctgcgc
     4621 ggcggtgcga cctcgccctg gccggccgtg ctcgctctgc tgattctggg tgcaaccgcc
     4681 gccgcgctcg caccgcgcca gacgcccgtc agcggcgaga gccaacttcc ggcggcccgt
     4741 ttcgcgagcg ccgcgcacat catcgccgtg cgctgtgcgc cctgccattc ggcgacaccc
     4801 accgagccgg gtttctcggt ggcacccaag gggttgatgc tcgatacgcc gcaagacatc
     4861 ctgacccatg tggccgtcat tgcaccgcag gttcaaaccc gcgccatgcc gatcggcaac
     4921 ctcaccggca tgaccgaccc cgaacgcacg caacttctcg actggatcca acgtggcgcg
     4981 cctcactgag cgggctgcct tgaacggcat ggacacggcc gaagtgcacg cgcgcatttg
     5041 gagcgccatc atggaccaga gcctgccgcc ggagacacgc ttggtggaaa gcgaactgtg
     5101 cgagatcttt ggcgtcggca ggacccgcct gcgccaggtg ctgcaacgcc tcgcgcacga
     5161 gcgggtggtc accttgatgc gcaaccgcgg tgccatggtc tcgagaccgt cgattcgcga
     5221 ggcgcgcgaa gtgttcgccg cgcggcgggt catcgaggcg aatgtcgtcg agacctttat
     5281 caagacagcg acccgcaagg acgtgaaacg cctccacgaa cacttggcgc gcgaaatcga
     5341 ggcatggcgg cgcaacgacc ggcgcgccat gctgaagctc tcgggcgagt tccatctgct
     5401 gctggccgaa gcggccggca acagcatcct gctcgggctg ctgcgcgacc tggtctcgcg
     5461 ttcctcgctg atcattgcgg tctaccaggc gccgggtgcc atgccctgtc cgccggacgc
     5521 gcatcgcgaa ctcacggaga ccttggagcg gcgcgagcgc aacgcggtca aattgatgct
     5581 ggcgcatctg gaccatgtgt tcgaggatct caccctcgag gaacgcagcg acggcggcgt
     5641 cgatctgaag agcgtgttcg gaggccggca atgacctatg cgcgtgatct tctcggctac
     5701 ggccgcaaag cgccgcacgc acgctggccg cacggtgcac gcatcgcctt gagtttcgtg
     5761 ttgaatcatg aggaaggcgg cgagaattgc gtgctcgatg gcgatgcggg gtccgagacc
     5821 tttctctccg aaatcatcgg cgcgacggcc tttccgatgc gccacatgag catggagtcg
     5881 ctgttcgagt acggctcgcg cgcaggtctg tggcgcgtgc tgcgtgcttt caaagagaga
     5941 cgcttgccct tgacggtgtt cgcggtggcg cgcgcactcg agcgccatcc ggaggccgtg
     6001 gcggcgtacc gcgaactcgg ccacgagatt gccgcgcatg gactgcgctg gatcagttat
     6061 caactcatgg acgaagccac ggagcgagcg cacctggcgg aggcggtggc cattctcacg
     6121 ggactcacgg gcgccgcccc cttgggttgg tacaccgggc gcgacagtcc gaatacccgg
     6181 cggctgattg tcgaacacgg cggctttctc tacgactccg attcctatgc cgacgatttg
     6241 ccgtattgga cccacgtgcc caagggcgcg gcgctgacgc cgcatctggt cgtgccctat
     6301 gccttggaca gcaacgacat gcgattcgcc gccctgcaag gcttcaattc aggtacccag
     6361 ttctttgact atctgcgcga ctccttcgac accctgtacc gcgaaggcga cccgctcggg
     6421 ctcgatgcgc ccaagatgct gtcgatcggc ttgcatgcac ggctctgcgg ccggcccggc
     6481 cgcatcgcgg ccctcgagcg ctttctcgac tacgtgggca cgcacgagcg cgtgtggatc
     6541 tgccggcgaa tcgatatcgc gcggcactgg gccgcgacgc atccgccgtg attacgctca
     6601 ctcatctcaa ccgcatgtcg ggcgacgaat ttgtcgcatc cgtggggccc ctgttcgaac
     6661 attcgccgtg ggtggctgag cgtgccgcgg cgctgcgtcc cttcgccagc gccctcggcc
     6721 ttcaccggac gatgtgcgat gcgctcatgc aggcctccgc ggatgagcag ctggccttga
     6781 tacgcgccca tccggagctt gcgggacgcg cggcgctcaa aggcgggctc acggcggcct
     6841 cgaccggcga gcagaagggt gccggcctct cggcctgcac cgcgcagcag ctgcagcggc
     6901 tgcagtcgct caattccgaa tatcgcagcc gctttggatt tcccttcgtg ctggcggtca
     6961 agggccacac gcccgacagc gtgattgccg ccctggccga gcgggtaact cacgacttga
     7021 acgaggagcg acaggtcgct ttgcgcgaaa tctgtcgaat agcgcgcttt cgcgtggcgg
     7081 atctggtgga cgagccgttg ggacaggcca tcatggcgat ggccgaggat ctcgccacct
     7141 tgagcgagac ggacgatgct ctcacctgct cctacctcac gccgacgcat cgcgccacgg
     7201 cggcacgcat tcgcgattac atgctggcag cgggtcttac ggcgcacatc gacgcggtgg
     7261 gcaacgtcgt cggccggctc gacggcgacg gtcgctccca ccaacgggta ctcaccggct
     7321 cgcactacga cacggtcatc aatgccggta aatacgatgg acgactcggt gtcgtactgc
     7381 cgatcgccgt cgccgggtgg ctgcgccgca gcggcgtgaa gctcgcttac ccgctcgaga
     7441 tcttgggctt tgccgaggag gaaggcgtgc gcttcaaatc gacatttttg gggagccgcg
     7501 ccattgccgg acgattcgac cccacggtgt tggacagcct cgatgcggcg ggcgtcacct
     7561 tgcgtgaggc gatcctcgcc gccggccacg acgtggcggc cattcccgcc atcgccagaa
     7621 accccgccga tgtggcgtgc ttcgtcgagg tgcacatcga acaaggaccc gtgctgctcg
     7681 cggcatcgga gcctgtgggt gtagtcaccc gcatcgccgg tagcctgcgc agcatggtga
     7741 cgatcaccgg catgtccggc cacgcgggca cggttcccat gtcgttgcgg catgacgccg
     7801 ccgccgccgc cgcggaaatc gtcctggccg tggagcgccg ctgcagcgcg caacccggcg
     7861 tggtcggcac ggtgggcaag ctcgaggtgc cgggaggtgc ggtgaatgtg attccgggac
     7921 gctgcgattt gtccatcgat atccgctcgg gtgaggacgc gctgcgcgac gcggcggatg
     7981 ccgacatccg cgccgagatt caacgaatcg cgacgcggcg cggcgtttcc gcgacgcaag
     8041 tccgggtact cgatgccgca agcgtcgcct gcacgccggc gctgcaagaa gcgtgggccg
     8101 cgagcgcctt gcgcgtgacc ggccgcgagg cgccgcgcct cgcgagcggc gcgggacacg
     8161 atgccatgat gatggcgagc ctcaccggca tcggcatgct gttcgtccgc tgcggcaacg
     8221 gcggcatcag tcatcatccc caggagtcga tgagcgccga cgatgccgat gtcgccgcgc
     8281 gcgtcttggt ggatttcttg cagcacgtgc aaagccccgc atgacctcgc ttaggctcgc
     8341 cgtcgaacgc gcgtttctcg gcccggcgct gcatgcgccg acgcacaccg agcttgagta
     8401 cctgccgaat gcgctcatca tcgtcgatca gcagggcgtc attcgggcgg tgctggggtc
     8461 gcagacgccc gaggctgcgg ccctgagcga gcgccaccgg gccgccggca cgctggtgac
     8521 gctcgagccc ggccagtatc tgctcccggg actcatcgat ctgcatgtgc acgcgccgca
     8581 atggccgcaa cttggatcgg cgctcgacct gccgctcgag gagtggttgc agtcgtgtac
     8641 ctttccactc gaggcgcgct tcgcggacac gcaattcgca cgcaccacct acgaatcgct
     8701 ggtcgcggga ctgctcgcca acggcacgac caccgcggtg tatttcgcga ccatccattt
     8761 ggcggcgacg caggcgttgg ccgacatctg cctctcgcgc tgccagcgtg cgctcatcgg
     8821 ccgcgtcgcc atggacgacc cgggacaatg ccccgcgttc tatcgcgaca gctccgccca
     8881 gctggccgaa gccgaaacgc gcgcattcat cgagtatgtg cgcgcgctgc ccggcaacga
     8941 gcagcggctg atcctgccgg tgatcacgcc gcgcttcatc ccctcctgca ccgatgcatt
     9001 gctcgcggga ctcgggcggc tggcacgcga gagcggctgc cacgtccaga cccattgctc
     9061 tgaaagcgat tgggaacatg cctacgtgct cgcgcgctgc ggcatctcgg acaccgccgc
     9121 tctcgagcgc ttcgggcttt tgtcccggca taccatcctc gcgcacggca acttcatcgg
     9181 cgctgccgat gcggccacga tcgtggccgc cggtgccggc gttgcgcatt gtcccttgtc
     9241 gaatgtgtat ttttcggacg ccgtatttcc gctgcgccgc ctgttgcaac agggcatgca
     9301 cgtcggactc ggcacggata tttccggcgg cgcaagcccc tcgattctcg agaacgcgcg
     9361 tcaggccatt atcgcctcgc gcagcctcga atccggcgtc gattccaccc tcgcgcggac
     9421 gcagcgccgc actcccgact cgcgcatcga tgccacgacg gcattctggc tggcgacggc
     9481 gggcggcggg atcgcgctcg acctgcccat cggattattc cgcgcgggct tccaattcga
     9541 tgccatcgtg atcgatacgc aggcccccga cagcaacctc aagctggagc agtccgcggc
     9601 gcccggccag atcttgcaga agatcattta tcacgccgca cgcagcaaca tccactcggt
     9661 gtgggtggcg aatcgccgcg ttcacgcgcg cgacgccgtt taagcctgca cctggcggtc
     9721 gatgtcagcg aaccagcgct ccttttgggc gggggtgagc agcgacgctt cgatcgaatt
     9781 gcgcgcgagc gtcgccagtt cctcgcgcga caaatcgagg ccctgctgaa ccgcgaggta
     9841 gttctcgagc agatagccgc cgaaatacgc cgggtcatcg gaattgaccg tgacgcacag
     9901 gccgcgctcg agcaggcgct tgagattgtg caattcaatg cgctcgaaga ctttcaattt
     9961 gatgttcgac aacgggcaca ccgtgagcgg tatgcgctgc agtgcaaggc ggcgcatgag
    10021 tgcctcgtct tcttcgcagc gcacgccatg atcgatacga aacacctgca acgcatcgag
    10081 cgccccgtgg atgtaggccg cggggccttc ctcgccggcg tgcgcgacgc ccggcaatcc
    10141 ttcctgccgc gcgcgggcga acacctcggt gaacttttcc ggtggatgcc ccgcttccgt
    10201 cgaatcgagg cccaccgccg cgatcaggct cttgtgaggc agcgcctcct cgagcgtgcg
    10261 catggcgtcg gccgcgctca aatgccgcag aaaacacagg atcaggcgcg acgtgatgcc
    10321 gaattctgcc tcggcctcat gcagggcgcg ccacatgccg tgcaattggg cggcgatcgt
    10381 cacgccgcgc gcggcgtgcg cctgggggtc gaaaaagatc tcgacgtgca ccacgccctg
    10441 gggatgtatt ttctcgagat aggcccgggt cagccaataa aaatcctcgg catcgcgcag
    10501 cacgtcggag ccggcgtaat aaatgtccag aaatgactgc agatccgtga actgatacgc
    10561 gcggcgcagc tcctcgacgc cgccgtacgg cagcgtcacg ccatgcttcg cggccagtcg
    10621 aaacgcgagc tccggctcga gcgtgccctc gatatgcaca tgcaattccg ccttgggtag
    10681 cgcgcggatg tactcacgga tggaggtttc ggcactcacg ccgccgacac taccacagcg
    10741 cgagctgccc cggccccgca agatccgcta tgcttgggca atgaaatcca ttcgcatcac
    10801 cgccggcggt cacaccttca tcgccgtccc gcatcccgag gcgccgaaaa ccgtggccgc
    10861 cttcggcaag ttgctgccgt ataaacagca aatcattcac gtgcgctgga gcggcgaggc
    10921 ctgctggatt ccgctcggtg acttcgatct gaaaaccggg ttcgagaatc acaccagcca
    10981 cccctcggtg ggcgacgtgc tgttctatcc cggcggcttc agcgaaactg agatcctgat
    11041 cgcctacggc agctgcgcct tcgccagcaa ggtcggacag ttggcgggca accactttct
    11101 caccatcgtc gaaggcaagg agaacttgcg ggcgcttggc gtcgaggtgc tgtggaaagg
    11161 cgccaaggac attctgttcg agcagctcta ggcgcgatac ggaagccaaa tcgcatggtg
    11221 ctgattcgca acaaggaaca gtgcggcgtg agtttttggt acccgtttgg cttcaggccg
    11281 caacgcagcg gcgaagtggt cagaattatt gactggaaca aatatagaaa atggctgccc
    11341 ctgcgagccc ttctcaaggt tgtgctcatc ggctttggat tactcttgtt caatgatcgg
    11401 atttacaccg acgatcgctt taatctgttt gccctgctgg tattgatttc atcgatcgcg
    11461 agcctgtggg cattcatccc gcgggggaca atcacagtca ccccgaccac gtcggtgagg
    11521 ctgcttccgc tcgccggcgt cgaatttgcc ggaaactttt tggcactggg aataatgata
    11581 ttggttttcg taggccagtc cgatgaagta cccgtcggcg cacaagggta cgatctggca
    11641 cagttttgta cgcacctctg gccgttggcg attttcctgg ttctttttgg gttattgccc
    11701 attgtcgtcg ccagcgcctt tgtcatgaga ttcggtcgat ccgcgccctg atgtcgaatt
    11761 agccgatgct cgcgcctaac ttgttgattc aataactagc cgccggctgc ggccgtgaaa
    11821 agcccttcgc gctcgatgaa gtcgaccacg gtggcaagcc cctgccccga cttcaaattc
    11881 gtaaatacga acgggcgctc gccgcgcatt ttcttggcgt cccgctccat cacctcgagc
    11941 gatgccccga ccatgggcgc gagatcgatc ttgttgatca ccagcaggtc cgatttggtg
    12001 attcccgggc cgcccttgcg cggaatcttg tcgcctgccg cgacatcgat cacgtacagc
    12061 gtgagatcgg acagttccgg actgaacgtt gccgccagat tatcgccgcc gctctcgacg
    12121 aagatcagat cgagtcccgg gaagcgctgc gacaggcgcg aaaccgcctc cagattgatg
    12181 gaagcatctt cgc
//
LOCUS       DASWZT010000574         7865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_67552, whole genome shotgun sequence.
ACCESSION   DASWZT010000574 DASWZT010000000
VERSION     DASWZT010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7865
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67552"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /gene="puhB"
                     /locus_tag="VGE92_16410"
     CDS             <1..558
                     /gene="puhB"
                     /locus_tag="VGE92_16410"
                     /inference="COORDINATES: protein motif:HMM:NF040894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030096 - plasma membrane-derived
                     thylakoid photosystem II [Evidence IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="photosynthetic complex putative assembly protein
                     PuhB"
                     /protein_id="HEY1315467.1"
                     /translation="MRAYHVRKVAAYFSLLVLWRIAVGVESGHSASAIVISCAFLFAL
                     GGIAIAVLSLLAYCAARATVYSITSRRVLLRHGVAVPMTMNIPFKVIDSAQLKMFPDR
                     TGDIAMRVIKAERVGYLITWPHLRPGFLTHPQPSLRALPDARRAAEILGLALAADAGV
                     AALRIDATGTVPAANGLRPRSAAAA"
     gene            572..1048
                     /gene="puhC"
                     /locus_tag="VGE92_16415"
     CDS             572..1048
                     /gene="puhC"
                     /locus_tag="VGE92_16415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="photosynthetic complex assembly protein PuhC"
                     /protein_id="HEY1315468.1"
                     /translation="MPAVVIGKQEKSLPRGALLGAGSLVLASLLLVSIARITGYGPAR
                     PPPSAVVERHDLRFEDRADGAVLIYSFATNRLVDTLPPGTNGFVRGVLRGLVRERRAE
                     HVGPTPPFRLTRWADGRLSLDDPSTGRHVDIEVFGPTNAGAFADILIASSRPKDKS"
     gene            1045..2112
                     /gene="acsF"
                     /locus_tag="VGE92_16420"
     CDS             1045..2112
                     /gene="acsF"
                     /locus_tag="VGE92_16420"
                     /EC_number="1.14.13.81"
                     /inference="COORDINATES: protein motif:HMM:NF010172.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048529 - magnesium-protoporphyrin IX
                     monomethyl ester (oxidative) cyclase activity [Evidence
                     IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-protoporphyrin IX monomethyl ester
                     (oxidative) cyclase"
                     /protein_id="HEY1315469.1"
                     /translation="MSNAVIGIGSGSEATRKAEANTLLSPRFYKTDYAALEHFDAAPV
                     RAEWDQMIDEFKLDINRDHFERNAQFDAEVARLPADLHQEFLDFLISSVTAEFSGCVL
                     YSEIKRRVANPELRELMGFMARDEARHAGFINQALKDFGVGVDLGFLRRDKKYTHFRP
                     KFIYYATYLSEKIGYARYITIFRHLERHPERRFHPIFRWFERWCNDEFRHGEAFALIM
                     RANPHLTRGHNKLWIRFFLLAVFATMYVRDHDRPLMHAAFDIDPTEYDFTVFRITTEI
                     SKQVFPLSLDLENPHFRRGLERLRTIAAASAAAKARGGALAALRRLGLAAAAAATFAR
                     LYLLPVRRHDLPAQVRMAPAW"
     gene            2123..2947
                     /gene="puhE"
                     /locus_tag="VGE92_16425"
     CDS             2123..2947
                     /gene="puhE"
                     /locus_tag="VGE92_16425"
                     /inference="COORDINATES: protein motif:HMM:NF023711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative photosynthetic complex assembly protein
                     PuhE"
                     /protein_id="HEY1315470.1"
                     /translation="MSGFGAPSLYAVLVWWFTTGIILFLDGLPRTSFRWSMAGATVIL
                     LAAGHALQLSAADASVRGAYTAFSAAILVWGWLEMSFLMGFITGPRKHACIERCCGWR
                     HFVHATQAIIYNEIATLLGALAVFGATYAAANRVALSTYAILWAMRLSAKLNLFLGVP
                     NLGEKFLPAHLQYLKGFFCKRPMNFLFPFSVTGATLVGARLVQKYLSAADAFQSTSYA
                     LLSSLLALAVLEHWFMVMPLPSERLWIWAFKAGRGTRGRDHVDRQLAAKAPYHPWS"
     gene            3014..3589
                     /locus_tag="VGE92_16430"
     CDS             3014..3589
                     /locus_tag="VGE92_16430"
                     /inference="COORDINATES: protein motif:HMM:NF013306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEY1315471.1"
                     /translation="MSAIAQLRSATSSSHQRLEKRLDVKARFTDLSAYRAHLEKMWGF
                     CAALEARLAPQSFGGALPDYEARRKLPLLTHDLVDLGEAAGGVASLPRCAALPQEMDA
                     AAAFGCVYVLEGATLGGRTLLPLVHSRLGFTPSHGARYLASYGDNVAAMWRDFGAALD
                     SWCCVAQRRYTAMQAAVATFELLAEWLCEAP"
     gene            3586..5838
                     /locus_tag="VGE92_16435"
     CDS             3586..5838
                     /locus_tag="VGE92_16435"
                     /inference="COORDINATES: protein motif:HMM:NF012580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY1315472.1"
                     /translation="MSDPAVRFGEADLTTCDREPIHIPGSIQPHGVLLVIDRQRQIVE
                     QVAGDSKSMLGVDPDGMPGLPISAILEPDTFALVSARLRSTAVFVPPLMRLGVRSRSG
                     PVPLDLTLQASDGTAIIELEPARRMMASAGDPIEQLKSLLAAVQHTSSLQECCAAAAV
                     TMRAATGFDRAMVYRFLPNGSGVVAAEDANAGLELFLGLHYPASDIPKQARELYRRNW
                     LRAIPNIDYVPAPLRPDTNARTRQPIDMSHCDLRSVSPIHLEYLRNMGVCASLSASII
                     CQDELWGMLVLHHYSPRYVSADLRVACETFAQILSLHVEAKVHAESSVVRIKARQIRE
                     RLVERLIGASDIAAVLASWDLLHYLGATGAVVYLEGQLHRVGETPSTAEILMMMQWLN
                     GINRPLFATEFLSSEYAPAADFAAVASGMVAVGLSRVPRDYVLWFRPEMGRTVRWAGD
                     PSKPIKVDRHGARLTPRGSFAEWLEVTKMQSAPWSEVDLESAEALRVVLLESVLKSVD
                     MARRERAFEETRIIAEELEKRVAQRTEQLRALAADLEAVEERERRQIARDLHDDLGQI
                     LSAARIRLTSLCQHEATDVSGTAMQVDALINRANNAIRSLASQLAPDVLHELGLAPAL
                     EWLAEEIEGTFGIKVRVRDDGRPKPLAAEARSILYRAVRELLINVAKHAQTDSATVDA
                     ECQAERVMVKVSDPGVGFAENALPPGQRRRLGLISLRERLSHIGGTMQLTSTPGAGTV
                     AILTAPLEQP"
     gene            5835..6473
                     /locus_tag="VGE92_16440"
     CDS             5835..6473
                     /locus_tag="VGE92_16440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY1315473.1"
                     /translation="MTTRVLLVEDHRMVREALSEVLKKVPDIDVVGEAGDAHEALKQA
                     TSLKPDVIVLDIRLPDVNGIEVAARLRDADISAKIVALSAFADKRFVTAMLRAGASAY
                     VTKSAAGTELVRAIRAVAAGQGYFSPEIAAELVSELRDRPLAGEAAPLARREREVLRL
                     IAEGVRSPAIAEQLHVTVGTVEVHRRNIMRKLGLRTVAELTKHAIREGIISL"
     gene            6568..7353
                     /locus_tag="VGE92_16445"
     CDS             6568..7353
                     /locus_tag="VGE92_16445"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY1315474.1"
                     /translation="MRMLPLYQWLVLGPHGTEGNSVEAGAPAPLPSDWLFNASNEPVL
                     IVNATTTNIVQANPPAAALFGQVYSELAGTLLVAAFEKSSGAEVRRCLDLARSAGSAS
                     SAVLRTQHGSAQVRAQLSLVRSPSDSFFLVRLDPDVRRPVSGAARSLAFEAIDRAVVG
                     FVLTESDFHIDFANRAFAQMVEAGSAAEVRGSSLLGWLRLTAEDLSRLRAQLTQREAA
                     TLLTTQLCPVGKRPRRVEVCAIPVPDAVNQCWGFTIRNLPRLN"
     gene            7414..7707
                     /locus_tag="VGE92_16450"
     CDS             7414..7707
                     /locus_tag="VGE92_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315475.1"
                     /translation="MSNRRFITPLPGCSDAGRGAGPCACMPHDSSERERMSRLARTIE
                     SEIVPRLLISSTVSQRSGLLAEDAAQLTRLLAALTQLQTALLKVSAAAQDSKT"
BASE COUNT         1334 a         2520 c         2515 g         1496 t
ORIGIN      
        1 atgcgtgcct accacgtgcg caaagtggcc gcttactttt cgcttttggt attgtggcgc
       61 atcgctgtcg gggtcgaaag cggccactca gcgtcggcga tcgtgatttc ctgcgccttc
      121 ttattcgcct tgggcggcat cgcgatcgcc gtcctgtcgc tgctcgccta ctgcgccgcg
      181 cgcgccacgg tgtacagcat cacgagccgc cgagtgctgc tgcgccacgg cgtcgccgtg
      241 ccgatgacga tgaacatccc tttcaaggtc attgacagcg cgcaactcaa gatgtttccc
      301 gaccgcaccg gcgatatcgc aatgcgggtg atcaaggctg agcgggttgg atacttgatc
      361 acctggccgc atttgcggcc gggattcctc actcatccgc agccgagcct gcgggcgtta
      421 ccggatgcgc ggcgcgcggc cgagatcctt gggctggcgc tggcagcgga tgcgggcgtc
      481 gctgcgctgc gaatcgatgc caccggaacc gtgcccgcgg ccaacggttt gcggccgcgc
      541 agcgccgcgg cggcctgaag gaagtcgcgc gatgcctgct gtggtcatcg gtaaacagga
      601 gaaatcctta ccgcgcggcg cgctgctcgg cgcgggctcg ctggtgctcg cctcgctgct
      661 attggtgagc attgcaagaa tcacgggtta tggaccggcg aggccgccgc cgagcgccgt
      721 cgtggagcgc cacgatttgc ggttcgaaga tcgcgccgac ggcgccgtgc tcatctactc
      781 gtttgcaacc aatcgcctgg tcgataccct accgcccggc accaacggtt tcgtgcgcgg
      841 agtactgcgc ggattggtgc gcgagcggcg cgccgaacac gtcgggccca cccctccgtt
      901 ccggctgact cgttgggcgg acggacggct gtcgctcgat gacccatcaa ccgggcgcca
      961 cgtcgacatc gaagtgtttg ggcccaccaa tgccggcgca tttgccgata tcttgattgc
     1021 cagcagccgg ccaaaggaca aatcatgagc aacgcagtga ttggcatcgg ttccggttcc
     1081 gaggcgacac gtaaggccga ggccaatacg ctactcagtc cgcgtttcta caaaaccgac
     1141 tatgcggctc tcgagcactt cgacgccgcg ccggtgcggg ccgaatggga ccagatgatc
     1201 gacgagttca aactcgacat caatcgcgat cacttcgagc gcaatgcgca attcgatgcc
     1261 gaggtcgcaa gactgccggc cgatctgcac caggaattcc tcgacttcct gatcagctcc
     1321 gtgacggcgg aattctccgg ttgcgtgttg tacagcgaaa tcaaaagacg cgtcgccaat
     1381 ccggagctgc gcgaactgat gggcttcatg gcccgcgacg aggcgcgcca tgccggcttc
     1441 atcaaccagg cgctcaagga tttcggcgtc ggtgtggacc tggggtttct caggcgcgac
     1501 aaaaaataca cgcactttcg tccgaaattc atctactacg cgacatactt gtcggagaag
     1561 atcggctacg cgcgctacat cacgatcttc aggcatctgg agcggcatcc cgagaggcgt
     1621 tttcatccga tattccgctg gttcgagcgc tggtgcaacg atgaattccg gcacggcgag
     1681 gcgtttgccc tgatcatgcg ggccaacccg caccttacgc gcggccataa caaattgtgg
     1741 atacgatttt tcctgttggc cgtgtttgcg accatgtatg tgcgcgacca tgaccgcccg
     1801 ctgatgcacg ccgcctttga catcgatccg accgaatacg acttcaccgt gtttcgcatc
     1861 accaccgaaa tttcaaaaca ggtgttcccg ctgtctttgg atttggagaa cccgcatttt
     1921 cgccgcggac tcgagcgctt gcgcacaatt gccgcggcga gcgccgctgc caaggcacgc
     1981 ggcggcgcgt tggctgcgct gcggcgactc ggactcgctg cggcggccgc ggcgacgttt
     2041 gcgcgcctct acctgctccc ggtgcggcgc cacgatttgc ctgcacaagt ccgcatggcg
     2101 ccggcctggt aagcaacggc gcatgtccgg ctttggcgcg ccgtcgcttt acgcggtcct
     2161 ggtttggtgg ttcaccacgg gaatcattct gttcttggac ggcctgccgc ggacttcgtt
     2221 tcgctggagc atggccggtg ccacggtcat cctgctggcc gccgggcatg ccctgcaatt
     2281 gagcgccgcc gatgccagcg tcaggggcgc ttacaccgcc ttctccgccg cgatactggt
     2341 atggggctgg ctggagatga gctttttgat gggcttcatc accggtccga ggaagcacgc
     2401 ttgcattgaa cgctgttgcg gttggcgcca cttcgtgcat gccacccagg cgatcatcta
     2461 caatgagatc gcgacgctgc tgggcgcctt ggcggttttc ggcgcaactt acgccgcagc
     2521 caacagagtt gccctctcaa cctacgcgat tttgtgggcc atgcgcctga gtgccaaact
     2581 caacttattc ctgggcgtgc ccaacctggg cgaaaaattc ctgcccgcgc atctgcagta
     2641 tctgaaaggt tttttttgca agcggccgat gaatttcctg ttcccattct cggtcaccgg
     2701 cgcaacgctc gtcggcgcgc gcttggtgca aaagtacttg agcgcggccg acgcctttca
     2761 gagcacgagt tatgccttgc tgagcagcct gttggcactg gcggtgctcg aacattggtt
     2821 catggtcatg ccgttgccga gcgagcgctt gtggatttgg gcgttcaaag ccggccgcgg
     2881 cacccgcggc cgggaccatg ttgaccggca gctggcggcc aaggcgccgt accatccctg
     2941 gagctgatgt ggcaccatag gataagccta tgagcccatt aaagccaagc ggcggcgacg
     3001 ctatgtcgag atgatgagcg ccatcgcgca attgcgctcc gcgacatcgt cctcgcatca
     3061 gcgcctcgag aagcgcctcg acgtcaaggc gcgatttacc gatttaagtg catatcgcgc
     3121 ccacctcgag aagatgtggg gattctgcgc tgcgctcgag gcgcgcttgg cgccgcagtc
     3181 gtttggcggc gcgctgccgg actacgaggc tcgacgcaaa cttccgctgt tgacgcacga
     3241 cctggtcgac ctcggcgagg ctgcgggcgg cgtggcgtcg ctgccgcgct gcgcggcgct
     3301 gccgcaggag atggatgccg cggcagcatt cggttgcgtc tatgttctcg aaggcgcaac
     3361 cctcggcgga cgcacgttac tgcctttggt ccacagccgc cttgggttca cgccaagcca
     3421 cggtgcgcgc tacctggcaa gttacggcga caacgtcgcg gcgatgtggc gcgacttcgg
     3481 tgccgcgctg gacagttggt gttgcgtggc gcagcgccgc tacacggcca tgcaggccgc
     3541 ggtcgcgact ttcgagctgc tggccgagtg gctgtgcgag gcgccatgag cgacccggcc
     3601 gttcgtttcg gtgaggccga tctcacgacc tgcgaccgcg agcccattca catcccgggt
     3661 tcgatccaac cgcacggcgt gctcctggtg atcgatcggc agcggcagat cgtcgaacag
     3721 gtggcgggag attcgaagtc gatgctcgga gtcgacccgg atggcatgcc ggggttgccg
     3781 atctcggcga ttttggagcc cgataccttc gcgctggtga gcgcccggct gcgttcaacc
     3841 gctgtcttcg ttccgccgct catgcgactc ggagtccgat cgcgaagcgg gcccgtgcct
     3901 ctcgatctca ccctgcaggc gtcggacggt accgccatca ttgaactcga gccggcacgg
     3961 cgcatgatgg cgagcgccgg cgacccgatc gagcagctca aatcgctgct ggccgcggtc
     4021 cagcacacct cgagcctgca agaatgctgt gcggctgccg cggtgacgat gcgcgccgcg
     4081 accggattcg atcgcgccat ggtctaccgc ttcttgccga acggcagcgg cgtcgtcgcc
     4141 gccgaagacg cgaacgcggg actcgaatta tttctcgggt tgcactaccc ggcctcggac
     4201 attccgaagc aggcgcgcga actgtatcgg cgcaactggc tgcgtgccat tcccaacatc
     4261 gactatgttc ccgcgccgct gcgcccggac acgaacgcgc gcacacgcca accaatcgat
     4321 atgagccact gcgatctgcg cagcgtctcg ccgatccatc tcgaatatct gcgcaacatg
     4381 ggcgtctgcg cctcgctctc ggcatcgatc atttgtcaag acgaactgtg gggaatgctg
     4441 gttctgcacc actattcgcc gcgctatgta tcggcggatc tgcgggtcgc atgcgaaacg
     4501 ttcgcgcaaa tcctgtcgct gcacgtcgaa gccaaagtgc acgccgaatc ctcggttgtg
     4561 cggataaagg cgcggcagat acgcgaacgg ttggtggagc gcctgatcgg cgcgagcgac
     4621 atcgccgcgg ttctcgcctc ctgggatctg ttgcattatc tcggcgcgac cggcgcggtc
     4681 gtgtacttgg aggggcaatt gcaccgggtc ggcgagactc cgtcgactgc cgaaatcctg
     4741 atgatgatgc aatggctcaa cggcatcaat cggcccttgt tcgccaccga gtttctctcc
     4801 tccgaatacg cgccggcggc cgatttcgcc gccgtcgcca gcggtatggt tgcggtcgga
     4861 ttgtcgaggg taccgcgtga ttacgtgctg tggtttcgtc cggaaatggg acgcaccgtg
     4921 cgctgggccg gagatccatc gaagccgatc aaagtcgacc gccatggcgc gcggctgacg
     4981 ccgcgcggct cgtttgccga gtggctggaa gtgaccaaga tgcagtcggc gccctggtcg
     5041 gaggtagacc tcgagtccgc cgaggccctg cgcgtggtgc ttctcgagag cgtactgaag
     5101 agtgtggata tggcacggcg cgagcgcgcc ttcgaggaga ctcgaatcat tgccgaggag
     5161 ctcgaaaagc gcgtggcgca gcgcaccgag caactgcgcg ccctcgccgc cgacctcgaa
     5221 gccgtcgaag aacgcgaacg gcgccagatt gcgcgcgatc tgcacgatga tctcggccag
     5281 atcctctccg ccgcccggat ccgcctgacc agcctctgcc agcacgaggc gacggatgtc
     5341 tccggcacgg ccatgcaggt cgacgccttg atcaaccgcg ccaacaatgc gatccgctcg
     5401 ctggcctcgc aactggcccc ggacgtgctg cacgagctcg gattggcgcc ggcattggag
     5461 tggctggcgg aggagatcga ggggacattc ggcatcaagg tgcgggtgcg cgacgatggc
     5521 cgcccgaaac cgttggctgc ggaggcgcgt tcgatcctgt accgcgccgt gcgggaactg
     5581 ctgatcaatg tggccaagca tgcacagacg gattccgcaa cggtggacgc cgagtgccag
     5641 gccgaacgcg tcatggtcaa agtgtccgac cccggcgtag gcttcgcgga aaacgccttg
     5701 ccgccgggtc aacggcgccg cctcggactg atcagcctgc gcgagcgctt gtcgcacatt
     5761 ggcgggacga tgcagctcac ttccacaccg ggcgccggca cggttgccat cttgaccgcg
     5821 ccattggagc agccatgacc acaagggttc tgttggtcga ggaccaccgc atggtgcgcg
     5881 aggcgttgag cgaggtgctg aagaaagtgc ccgacatcga cgtcgtcggt gaagccggcg
     5941 acgcgcacga ggcgctgaaa caggccacca gcctgaagcc cgacgtcatc gttctcgaca
     6001 tccggcttcc ggacgtcaat ggcatcgaag tcgccgcccg cttgcgggac gccgacatct
     6061 cggcaaaaat cgtcgcgctc tcggctttcg cggacaagcg cttcgtgacg gcgatgctgc
     6121 gcgcgggagc gtcggcttat gtgacgaagt cggctgccgg taccgagttg gtgcgggcca
     6181 tccgcgccgt tgctgccgga cagggttatt tctcgccgga aatcgccgct gaattggtca
     6241 gcgaattgcg cgatcgcccg ctcgccggcg aggcggcgcc actggcacgc cgtgaacgcg
     6301 aggtgctgcg cctgatcgcg gaaggggttc gttcacctgc cattgccgag cagctgcacg
     6361 tgacggtcgg cacggtcgaa gtgcatcgtc gcaacatcat gcgcaagttg ggcctgcgca
     6421 cggtggccga gctcaccaag cacgccatcc gcgagggcat catctccctc tgatcgggct
     6481 cggatccacg accctaccgg gttcccggta gtcagccctc cgggaagcgg gaatgtgcgt
     6541 ggggacgcac ggcgcatagg ctttgccatg cgaatgctac ctctatatca atggctggtt
     6601 ctaggcccgc acggcaccga ggggaattcg gtcgaagcgg gcgcgcccgc gccgctcccg
     6661 agcgactggt tgttcaacgc ctcgaacgaa ccggtgctga tcgtaaacgc caccaccacg
     6721 aacatcgtac aagccaatcc acccgccgct gccctgttcg ggcaggttta ttcggaactc
     6781 gccggcaccc tgttggtggc ggcgttcgag aaatcgagcg gcgccgaagt ccgccgctgt
     6841 ctggacctcg cacgaagcgc gggcagcgcg agcagtgcgg tcttgcgaac gcagcacggc
     6901 tccgcccaag tccgggcgca actctcgttg gtgcgctcgc cctccgactc gttcttcttg
     6961 gtcaggctcg accccgatgt acggcgaccc gtcagcggcg ctgcccggtc gctggccttc
     7021 gaggccatcg atcgcgcagt ggtcggtttc gtactgacgg agtccgattt tcatatcgat
     7081 ttcgccaatc gagccttcgc gcaaatggtt gaggccgggt ccgcggccga ggttcgcggc
     7141 agctcgctgc tcggatggct gcgactcacc gcggaggatc tgtcgcgctt acgcgcgcag
     7201 ctgacgcagc gcgaggcagc gactctcctg accactcagc tatgccccgt gggcaagcgc
     7261 ccccgccggg ttgaagtttg cgcgataccg gtacccgacg ccgtcaatca atgctggggt
     7321 tttaccattc ggaatctgcc gcgcctcaac tgagttgcct cgcggcccct tcccgagtaa
     7381 ggtctcgtgc gaaccgaaaa ggacaggggc caaatgtcta atcgacgttt catcacgcct
     7441 ttgcctggct gcagcgacgc cgggcggggc gctggcccgt gcgcctgcat gccgcatgac
     7501 tcgagcgagc gcgaacgaat gtcccgtttg gcacggacca tcgagagtga aatcgtccct
     7561 cggctgctga tttcttcgac cgttagccag cgctccggtc tgctcgccga ggatgcagcg
     7621 cagctcaccc gactgctcgc ggcgctgacg cagctgcaga ccgcgctgct gaaagtcagc
     7681 gcagcggcgc aagacagcaa gacctagaat tcggttaccg gccgctgcct tgcaaagacg
     7741 cggtcggcgg cgagctggcg gggggcggcg cgacgtgcat ttcgccttgc agttctgggt
     7801 aatccttcgc catgctctcg ccgtacagcg gcttgaacac gccctgatga caggtggcgc
     7861 agttg
//
LOCUS       DASWZT010000575         2992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_77180, whole genome shotgun sequence.
ACCESSION   DASWZT010000575 DASWZT010000000
VERSION     DASWZT010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77180"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VGE92_16455"
     CDS             <1..814
                     /locus_tag="VGE92_16455"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HEY1315476.1"
                     /translation="WQANLDLSFARREGRTLLTRRSHTGPLQIQKVLYPEGREICHAT
                     VLHPPGGIAAGDSLNIAAQLDAGARVCLTTPGATKWYRCPQGESQQQLRFSLGEGAAL
                     EWLPRENILFDASEVGMGLDVDLGPSAQFIGWELLCFGRRASRETWRHGGLNLRTSIR
                     QAGRLLWCERGQVRADSGFDCSAVGLAGCSVSGTLIAAGAPIDSATLTACRSIVPGEG
                     SRIGISALPQVLVARYLGHASQDAFNWFVAVWHVLRPALLGVAASAPRIWAC"
     gene            831..1133
                     /gene="ureA"
                     /locus_tag="VGE92_16460"
     CDS             831..1133
                     /gene="ureA"
                     /locus_tag="VGE92_16460"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006811999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEY1315477.1"
                     /translation="MELTPREKDKLLIFTAGLLAERRRARGLKLNYPETVALITCAIL
                     EGARDGKTVAELMSYGSTIITRAEVMEGIAEMIPQIQVEATFPDGTKLVTVHHPIV"
     gene            1146..1451
                     /locus_tag="VGE92_16465"
     CDS             1146..1451
                     /locus_tag="VGE92_16465"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004644206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HEY1315478.1"
                     /translation="MIPGEILCAPGDIELNADRPTLDVHVENTGDRPIQVGSHYHFYE
                     TNLSLKFDREPTRGYRLDIAAGTAVRFEPGQGRSVRLVRYGGLKRVYGFQGKVMGAL"
     gene            1453..>2992
                     /gene="ureC"
                     /locus_tag="VGE92_16470"
     CDS             1453..>2992
                     /gene="ureC"
                     /locus_tag="VGE92_16470"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF009686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEY1315479.1"
                     /translation="MAVKISRQAYAEMFGPTIGDRIRLADTALVIEVEKDLTCYGEEV
                     KFGGGKVIRDGMGQGQATSGAVADTVITNALIVDHWGIVKADVGLKNGAIWGIGKAGN
                     PDVQSQVSIPIGAATEIIAGEGLILTAGGIDSHIHFICPQQIEEALTSGVTTMFGGGT
                     GPAAGTSATTCTPGPWHIHSMLLAAEAFPMNLGFVGKGNVSMPEPAREQIRAGAVGLK
                     LHEDWGTTPAAIDNCLRVADEMDVQVAIHTDTLNESGYLEATLAAIGGRTIHTFHTEG
                     AGGGHAPDIIKAASHENVLPSSTNPTRPYTINTIDEHLDMLMVCHHLDPAIAEDIAFA
                     ESRIRRETIAAEDILHDIGAFSMMSSDSQAMGRVGEVIIRTWQTAHKMREQRGSLSGD
                     PATHDNARIKRYIAKYTINPAITNGIGHLVGSVEVGKLADLVLWKPAFFGVKPSLIIK
                     GGMIAAAPMGDPNASIPTPQPSHYRPMFGAFGRALEKCVTFVSGAALENPAFTNLGLK
                     KRLEP"
BASE COUNT          571 a          926 c          964 g          531 t
ORIGIN      
        1 ttggcaagcc aatctcgatt tgtcttttgc ccgccgcgag ggtcgaacgc tgctcacgcg
       61 caggtcgcac actgggccgc tgcaaataca gaaggtactg taccccgaag gccgcgagat
      121 ctgtcatgcg acggtcttgc atcccccggg aggcattgcg gccggcgact cgctcaacat
      181 cgcggcgcaa ttggatgcag gcgcgcgggt gtgcctcacg acgcccggcg cgaccaagtg
      241 gtatcgctgc ccgcagggcg agtcgcagca gcagctgcgt ttctcgctcg gcgagggtgc
      301 ggcattggaa tggctgccgc gagagaacat tctcttcgat gcgtcggagg tgggcatggg
      361 cctcgacgtc gacttgggac cgagcgcgca attcatcggt tgggagttac tctgtttcgg
      421 ccggcgcgcg tcacgggaaa cctggcgcca cggcggattg aacctgcgca cgagcattcg
      481 gcaagcgggg cgcctgctct ggtgcgagcg cggccaggta cgggccgaca gcggcttcga
      541 ctgctctgcc gtgggtctgg cgggatgcag cgtgagcggt acgctgattg ccgcgggcgc
      601 acctatcgat tctgcgacgc tcaccgcctg ccgtagtatc gtgccgggcg aggggtcgcg
      661 aatcggcatt tccgcgctgc ctcaagtctt ggtggcgcgc tatttgggcc atgcatcgca
      721 ggatgcgttc aattggtttg ttgccgtgtg gcacgtgctg cgtcccgccc tgttgggcgt
      781 ggcggcgtcc gcgccgagaa tttgggcttg ttaacggtat cgagtcagcc atggaattga
      841 cgccgcgaga gaaagacaaa ttattgatct tcaccgccgg actgctggcg gagcggcgcc
      901 gggcacgtgg cttgaaattg aactaccccg agaccgtggc gttgatcacg tgcgccatct
      961 tggagggagc gcgcgacggc aagacggttg cagaactcat gagctacggc agcaccatca
     1021 ttacgcgtgc ggaggtcatg gagggcatcg ccgagatgat cccgcagatt caggtcgaag
     1081 cgacctttcc cgacggcacc aagctcgtca ccgtgcacca tccgatcgtc tgaggagcga
     1141 cagcgatgat tccaggcgaa atactctgcg ccccgggcga catcgaattg aacgcggatc
     1201 gcccgactct cgatgtgcat gtcgagaaca ccggcgaccg gccgatccag gtgggctcgc
     1261 actatcattt ttatgagacc aacttaagtc tcaaattcga ccgcgaaccg acccgcggct
     1321 atcgcttgga tattgccgcg ggcactgcgg tgcgcttcga accgggccag ggcaggagcg
     1381 ttcgcttggt tcggtatggc ggcctgaagc gcgtctacgg ctttcaggga aaagtgatgg
     1441 gagctctctg acatggccgt gaaaatatcc cggcaggcgt atgccgaaat gttcggcccc
     1501 acgatcggcg atcgcattcg actcgcggac acggcgctcg tcatcgaagt cgagaaagac
     1561 ctcacctgct acggcgagga agtgaaattt ggcggcggca aggtgattcg cgacggcatg
     1621 gggcaggggc aggcgacgag cggtgccgtg gccgatacgg tgatcaccaa tgcgttgatc
     1681 gtcgatcatt ggggaatcgt caaagccgac gtcggactca agaacggggc gatctggggc
     1741 atcggcaagg cgggcaatcc cgacgtgcaa tcgcaggtca gcatcccgat cggggccgcg
     1801 accgaaatca tcgccggcga gggcctgata ttgacggcgg gcggcatcga cagtcacatc
     1861 cacttcatct gtccgcagca aatcgaggag gcgctgacct cgggtgtgac caccatgttc
     1921 ggcggcggca cggggcctgc cgccggcacc agcgcaacca cgtgcacgcc ggggccctgg
     1981 cacatccaca gcatgctgtt ggcggcggaa gcctttccca tgaacttggg attcgtcggc
     2041 aagggcaacg tcagcatgcc cgaacccgcc cgcgaacaaa tccgcgccgg cgcggtgggt
     2101 ttgaaattgc atgaagactg gggcacgacg ccggcggcga tcgacaactg cctgcgcgtg
     2161 gccgatgaga tggacgtgca ggtcgccatc cacaccgaca ccctgaatga atccggatac
     2221 ctcgaggcga cgctcgcggc catcggcggc cgtaccatcc acacctttca taccgagggt
     2281 gctggcggcg gccacgcgcc cgacatcatc aaggccgcct cgcatgagaa cgtgctgccg
     2341 agttcgacca atccgacgcg cccctacacc atcaacacca tcgatgagca cttggacatg
     2401 ctcatggtat gtcaccactt ggatccggcc attgcggagg acatcgcttt cgcagaatcg
     2461 cgcattcgac gcgagaccat cgccgccgag gacatcctgc acgacatcgg cgctttctcc
     2521 atgatgtcct cggattcgca ggccatgggc cgggtcggcg aagtcatcat tcgcacctgg
     2581 cagacggcgc ataaaatgcg cgagcagcgc ggcagcttga gcggcgaccc ggccactcac
     2641 gacaacgccc gcatcaagcg ctacatcgcc aagtacacca tcaatcccgc gattaccaac
     2701 ggtatcggcc acctggtcgg ttccgtcgag gtcggcaaat tggccgacct ggtgctgtgg
     2761 aaaccagcgt tcttcggcgt gaagccgagt ctcatcatca agggtggcat gatcgccgcg
     2821 gcgcccatgg gcgatccaaa cgcctcgatc ccgacgccgc agccctcgca ttatcgcccg
     2881 atgttcggcg ccttcggccg cgcgctcgag aaatgcgtga ccttcgtgtc cggcgccgcg
     2941 ctcgagaacc cggcattcac gaacctgggg cttaagaaac ggctcgagcc gg
//
LOCUS       DASWZT010000576         8789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_62790, whole genome shotgun sequence.
ACCESSION   DASWZT010000576 DASWZT010000000
VERSION     DASWZT010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8789
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62790"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VGE92_16475"
     CDS             complement(<1..367)
                     /locus_tag="VGE92_16475"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HEY1315480.1"
                     /translation="MQESIGAMTANQELWLGLDTGGTFTDAVLLANGRQVIASAKALT
                     TPWNLALGIGQAIAAVLAFLPERASRAHISLVSVSTTLATNAVVENRFSPVLSLLIGF
                     DDAMVERTELKRPGSGGLVG"
     gene            494..871
                     /locus_tag="VGE92_16480"
     CDS             494..871
                     /locus_tag="VGE92_16480"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY1315481.1"
                     /translation="MQRAVRKYLAALEANDVDKAAALFTPDGWVQSPFLGRLPVRAYI
                     AKVAAASKSSVLTVHDVLVSAEGHMRAVAYYLYDWQLKDGSKVTFECADVFNFDPDSG
                     RIASVVLVYDTHLVRPTVENKYP"
     gene            complement(887..1942)
                     /gene="bmt"
                     /locus_tag="VGE92_16485"
     CDS             complement(887..1942)
                     /gene="bmt"
                     /locus_tag="VGE92_16485"
                     /EC_number="2.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF005718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine--homocysteine S-methyltransferase"
                     /protein_id="HEY1315482.1"
                     /translation="MTDRLTSLLAHRPWLLADGATGSNLFDRGLQSGDAPELWNSEHP
                     ERIAQLHRAFVEAGADIILSNTFGGTRHRLKLHKAGGRVAELNEKAARIARTEADRSG
                     REILVGGSIGPTGEILEPLGPLSLEAARAAFAEQAAALARGGADLMWIETMSSVEETE
                     AAIAGARTTGLPVVATLSFDTNGRTMMGITPLELAALHRKHALAACGGNCGTGPSELV
                     ACLVNLATAADPAAVLVAKANCGIPQFVNGAIQFDGTPELMAEYVRLALDSGARIIGG
                     CCGTTPAHLRVMREALEAHVPGPKPDLKTIESRLGAISSGATAQLRGEFSREAGAAAG
                     ARARRTTSHRSVRAENP"
     gene            2222..3322
                     /locus_tag="VGE92_16490"
     CDS             2222..3322
                     /locus_tag="VGE92_16490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315483.1"
                     /translation="MREQKSSRLPLMRVVAATALGLSAALAVASEYQDAFIPKLINSS
                     TVPSNGDLNPYGVAFVPSGFPQGGLLSAGDVLVSNFNNSENLQGAGTSIVQLTPSGTL
                     APPGTALPFFTSRLAGLSTALGVLRGGFVIVGNVPTTDGTVATIGQGALQVIDRHGQW
                     VETLTDPKLLDGPWDLAVDDHGDWARLFVSNVLNGTVSRLDVAVAAKGVTLLKKTLIA
                     TGYPHVPNSAALILGPTGLAFDGATGKLYVASTADNTIYEISNAGSRSTEVVRGSVAF
                     ADEHLRGPLALRFAPNGNLLTANGDAVNADVLHPSEIVEFTTGGHFVREYNVDSSQGG
                     AFGLDTLSEGPFNYAVIDDVTNSLAVYRLRDR"
     gene            complement(3350..4102)
                     /locus_tag="VGE92_16495"
     CDS             complement(3350..4102)
                     /locus_tag="VGE92_16495"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1315484.1"
                     /translation="MTQVTVKRLWKEFGTQVVLENVNLHVDDHEFVTIVGASGCGKTT
                     FLRMLLGVEAPSRGEFLIDGEPLASEPGPDRGIVFQRYSLFPHMSVRQNLLLGLELRG
                     SPLFGRLFGATRRRALERADELLEAVGLQGARDKYPQQLSGGMQQRLSIAQSVICEPK
                     ILLLDEPFGALDPGITADMHTLIIKLWNMNKMTIFMVTHDLQESFELGTRLLVFDKIR
                     VDPQSPDAFGATITFNIPLKGRRAQIPEVARG"
     gene            complement(4099..4914)
                     /locus_tag="VGE92_16500"
     CDS             complement(4099..4914)
                     /locus_tag="VGE92_16500"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY1315485.1"
                     /translation="MRRLINQHPGRGGALGLSTLPFIVLIAAYLFASHARLAANPGDK
                     LLPAPATIAATMHRFAFEEDERSGEYLLWNDTGASLERLLTALGASALIGIILGVAIG
                     VLPYLRVTLAPLIAVVSLIPPLAVLPILFIVVGLGEMAKITLIIIGVAPVIARDLAMR
                     AQELPGEQIIKAQTLGASSWQLVLRVVLPQMWPRLVDSLRLTLGSAWLFLIAAEAIAS
                     TEGLGYRIFLVRRYLAMDVILPYVAWITLLAFTMDWLLRLLRAKAFAWAKAGG"
     gene            complement(4926..6002)
                     /locus_tag="VGE92_16505"
     CDS             complement(4926..6002)
                     /locus_tag="VGE92_16505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative urea ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY1315486.1"
                     /translation="MLTRLKDLLLIPLLGILAVGSALPAAAAERSSFNIAWSIYVGWM
                     PWDYADHSGILKKWADKYGIKIKLTQVNEYVESINQYTAGSFDGCVMTNMDMLTIPAA
                     GGVDSTALIVGDFSNGNDGVVLKGKGKTLGDIKGQKVNLVELSVSHYLLVRALSSAGL
                     RERDLKIINTSDADIVAAFSAPATTAVVTWKPQLSAVLAQPNAQLVFDSSKIPGEILD
                     LMVVKSDVLNANPKLGKALLGAWYETLGVMFRNDAAGRAAQTAMAQASGTDLAGFGLQ
                     LATTHVFATAADAYAFVTADGVIKSMDLVRKFSFEHGILGQGASSADVVGIQFPAGKT
                     LGDLHNLKMRFDSSFTKLAADGKL"
     gene            complement(6028..>8789)
                     /gene="uca"
                     /locus_tag="VGE92_16510"
     CDS             complement(6028..>8789)
                     /gene="uca"
                     /locus_tag="VGE92_16510"
                     /EC_number="6.3.4.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004847 - urea carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urea carboxylase"
                     /protein_id="HEY1315487.1"
                     /translation="TRLQVEHGVTEEVTGVDLVEWMVRQAADEDFALRTPQARGASIQ
                     ARLYAEDPARQFRPSTGLLSRVRFAANARIETWIEDGSVVTPFYDPLLAKIITVGADR
                     AAALSAMRAALAATDIGGIESNLDYLRQLVADPVFAGGRMTTRALESFAYAANTVEVL
                     AAGTQTTIQDYPGRLGFWNVGVPPSGPMDSLSFRLANRALGNSPRAAALEMTVSGATL
                     RFNSDATICLVGAAMAANLAGRTAEFGTPLAVRRGETLTLSAIQGAGCRSYLAIRGGF
                     DVPLYLGSRSTFTLGKFGGHGGRALMVGDVLHVGPARTQAGAHEKQPQAIPSDLMPAI
                     GNDWELGVLYGPHGAPDFFTDADIAEFFAATWEVHYNSSRTGVRLIGPKPKWARSDGG
                     EAGLHPSNIHDNAYAIGTVDFTGDMPVILGPDGPSLGGFVCPVTVVGAELWKLGQLRA
                     NDRVRFKRLSLEEAQQAEALQQSLVETLARPRASTSRMKRADAAADTPILAQFAARES
                     RPSVVYRRAGDRYVLVEYGALVLDLELRLRVHALMSWLQTTAPPGIIDLTPGIRSLQL
                     HYDDRVLPLAALLDVLRAGERALAATADRQIATRIVNLPLSWDDESTQLAIAKYMQSV
                     RADAPWCPSNIEFIRRVNGLDSVEEVKRIVFDANYLVLGLGDVYLGAPVTTPLDPRHR
                     LVTTKYNPARTWTPENAVGIGGAYLCIYGMEGPGGYQFVGRTCQVWNTYKVTAEFTHG
                     KPWLLRFFDQIRFFPVSGAELKEFRADFLHGRARLDIRDATFQLNEYRSFLAANAASI
                     AAHKMRQQSAFDSERRRWEATGQIGYAADLPAAQEADGEELPPGCVAVLSPVAGSVWK
                     ISSAPGQSVKAGDTLLLVESMKMELPVTAPLDGVVIQMRCVEGRAVLIAQTLVVMRPH
                     ERAAA"
BASE COUNT         1689 a         2817 c         2680 g         1603 t
ORIGIN      
        1 gccccaccaa cccgccgctg cccggacgct tgagctccgt gcgttcgacc atggcatcgt
       61 cgaatccgat cagcaggctc aagaccggac tgaagcggtt ctcgacgacg gcattggtgg
      121 cgagcgtggt cgaaaccgac acgagactga tgtgggcgcg gctcgcgcgt tcgggcagga
      181 aggccaatac cgccgcgatc gcctggccga tgcccagcgc cagattccac ggcgtcgtca
      241 atgccttcgc actcgcgatc acctgccggc cgttggcgag aagcaccgcg tcggtaaacg
      301 ttccgccggt atcgagaccg agccaaagtt cctgattggc agtcatggcg ccgatcgatt
      361 cctgcaaaat tagctccgca gcccgcggcg ccgcgtaaca taccggattc gcagcacacg
      421 cgctctgccc ctttctaaag taagcttgaa caattaagct cgatggccgg gcgttacagc
      481 atgggagagg catatgcagc gagcagtgcg caagtatctc gcggcccttg aggcgaacga
      541 cgtcgacaag gccgccgcgc tattcacgcc cgacggctgg gtgcagtcgc ctttcctcgg
      601 ccgcctcccg gtacgggcct acatcgcgaa agtcgcggcc gcctccaagt cctccgtgct
      661 gaccgttcac gatgtgctgg tgagcgcaga agggcacatg cgcgcagtcg cctactatct
      721 gtacgactgg cagttgaagg atggttccaa agtgacgttc gagtgtgccg acgtcttcaa
      781 ttttgacccc gactcgggcc ggatcgcctc cgtggtcttg gtttacgaca cgcacctggt
      841 acggcccacg gtcgaaaaca agtatccgtg agccgcgccc cggcgatcag ggattctctg
      901 cgcgcacgct gcggtgactg gtggtgcgcc tggctcgcgc cccggcggcg gcccccgctt
      961 cgcggctgaa ctctccgcgc aactgcgcgg tggcaccact cgaaatggcg cccagcctcg
     1021 attcgatggt cttaagatcg ggcttgggtc ccggaacatg agcttcgagc gcctcgcgca
     1081 tcacgcgcag gtgcgccgga gtcgttccgc aacaaccacc gatgatgcgc gcgcccgaat
     1141 ccaacgccaa gcgcacgtac tcggccatca attccggtgt accgtcgaac tggatcgcgc
     1201 cgttgacgaa ttgcggaatg ccgcagtttg cttttgcgac tagaacggcc gccgggtccg
     1261 ccgcagtggc gagatttacc agacacgcca cgagctcaga gggtcccgtg ccgcaattgc
     1321 cgccgcatgc cgcaagagcg tgcttgcgat gcagggcggc gagttccaat ggtgtaatgc
     1381 ccatcatggt gcggccgttg gtgtcgaagc tcaacgtggc aacgaccggc agaccggtgg
     1441 tgcgcgcgcc ggcaatcgcg gcctcggttt cctcgaccga tgacatcgtt tcgatccaca
     1501 tgaggtccgc accgccccgg gcgagcgccg cggcttgttc tgcaaaggcc gcgcgcgcag
     1561 cctcgagact caagggcccg agcggctcga gaatctcccc ggtgggaccg atgctgccgc
     1621 cgaccaatat ctcgcgcccg gagcgatccg cttcggttcg ggcaatccgt gccgctttct
     1681 cattgagttc tgcaacgcgg cctcccgcct tatgaagctt caagcggtgc cgagtgccgc
     1741 cgaaagtgtt gctcaggata atatccgcgc cggcctcgac gaaggctcgg tgcaactgcg
     1801 caatccgctc gggatgctcg gaattccata attcgggcgc gtcgcccgac tgcaatccgc
     1861 gatcaaacaa gttgctaccg gtcgcgccat ccgcgagcag ccaaggcctg tgcgccaaaa
     1921 gggaagtcag tcgatcggtc atgaacattc ttctgagaat aaacgcgcca ctctaacatg
     1981 ccgggtcgta agtcctgacg gacagacctt caggcccata gctaaaatcg atattataaa
     2041 taatctgtaa cctattgtta catcgacata tagaccgcgc tcaagcggcg gcgcaaacgc
     2101 ctcgctaggt gccgcgcgcc tcgacagccc gcgcggcaac gtgcgcgtcg cgtaatgaat
     2161 ttaaccattg gggccgatcg aggtacttac gcgtgtccga aatcaacaat aggggaactt
     2221 catgcgtgaa caaaaatcgt ccagattgcc tctgatgcgg gtcgtcgccg ccaccgcttt
     2281 gggtttatcg gcggctctcg ccgtcgcttc ggaatatcaa gatgccttca ttccgaagct
     2341 catcaattcc tcaaccgtcc cgtccaatgg cgacctcaat ccctacggcg tcgccttcgt
     2401 cccgagcggc tttccgcagg gagggttact ttcggccgga gatgttctcg tcagcaattt
     2461 caacaatagc gagaatctac agggcgcggg tacgagcatc gtgcaactca ctccgagcgg
     2521 cacgctcgcg ccgccgggaa cagccctgcc gttcttcacc agccgcttgg ccggcttgag
     2581 cacggcactc ggcgtgttgc gcggcggctt cgtgatcgtc ggcaatgtac cgaccacgga
     2641 cggcaccgtc gcaacgatcg gccaaggtgc tctgcaagtg atcgatcggc acggccaatg
     2701 ggtcgagacg ctgaccgatc ccaagctgtt ggacggtcct tgggatctgg cggttgacga
     2761 tcacggcgac tgggcacgtc tgttcgtgtc gaatgttctc aatggaaccg tttcccgcct
     2821 cgatgtggca gtggctgcga aaggcgtgac gctgctcaag aagaccttga tagcgaccgg
     2881 ctacccgcat gtgccgaatt ctgcggccct gattctcggt ccgaccggtc tcgccttcga
     2941 tggcgctacg ggaaagctct acgtcgcatc gactgcggac aacacgatct atgaaatatc
     3001 gaatgcgggc agccgtagta ccgaggtggt gcggggcagc gtggcgttcg ccgacgagca
     3061 cttgcggggt cccttggcgc tgcgctttgc gccgaacgga aatctgttga ccgccaatgg
     3121 cgatgcggtc aatgccgacg tcctgcaccc cagcgaaatc gtggaattca cgaccggggg
     3181 gcattttgtc cgcgagtaca acgtcgactc gagtcaaggc ggggcctttg gtctcgacac
     3241 cctgtccgaa ggaccgttca attacgccgt catcgatgac gtcaccaaca gcctggcggt
     3301 gtatcgcctg cgggatcgct agcgagggcg gggttgcgcg ggacgcgatt cagccccgcg
     3361 caacctccgg gatctgcgcc cgccgacctt tgaggggaat gttaaaggta atggtcgcgc
     3421 caaaagcgtc cggcgactgc ggatcgacgc gaatcttgtc gaaaaccagg agccgcgtgc
     3481 cgagttcgaa gctctcctgc agatcgtgcg tcaccatgaa aatggtcatc ttgttcatgt
     3541 tccagagctt gatgatgagg gtgtgcatgt ccgccgtgat gcccgggtcg agggcgccga
     3601 acggctcgtc cagcaacagg atcttcggtt cgcagatgac agattgcgca atcgacaatc
     3661 gctgctgcat gccccccgaa agctgctgcg gatatttgtc ccgggcgcct tgcaatccga
     3721 ccgcctccaa tagctcgtcg gcgcgctcca gggcgcgccg ccgcgtcgcg ccgaacaacc
     3781 ggccgaacag cggcgagccg cgcaactcca ggcccaacag caggttctgg cgcacgctca
     3841 tgtgcggaaa caacgaatag cgctggaaca cgatgccgcg atccggcccg ggctccgacg
     3901 ctagaggctc accgtcgatc aggaactccc ctcggctcgg cgcctcgacg cccagcagca
     3961 ttctcaagaa cgtcgtcttg ccgcagccgg atgcgccgac gatggtcacg aattcgtgat
     4021 cgtcgacatg caggttgacg ttctcgagca cgacttgagt gccgaactcc ttccacaagc
     4081 gtttcaccgt gacctgggtc atccgccggc cttggcccag gcgaaagcct tggcacgcag
     4141 cagccgcagt agccagtcca tggtaaacgc cagcaaggta atccacgcaa cgtacggcaa
     4201 gatgacatcc atggccaggt agcggcgcac cagaaatatc cgatagccga gaccctcggt
     4261 cgaggcgatg gcctccgcgg cgatcaggaa cagccatgcc gagcccagcg tcaggcgcag
     4321 cgagtcgacc aaccgcggcc acatctgcgg caacaccaca cgcagcacca gctgccagct
     4381 cgaggctccc aaggtctgcg ccttgatgat ctgttcgccc ggcagctctt gagcgcgcat
     4441 cgccaggtcg cgggcgatga caggcgcgac gccgatgatg atcagggtga ttttcgccat
     4501 ttcgccgagc ccgacgacga tgaacagaat cggcaggacc gcgagcggcg gtatcagcga
     4561 caccacggca atcagcggtg ccagcgtgac gcgcaaatac ggcaacacgc cgatcgcgac
     4621 gccgagaatg atgccgatga gcgcgctcgc gccgagcgcc gtcagcaatc gctcgaggct
     4681 cgcgccggtg tcgttccaca acaaatactc gccgctgcgt tcgtcctctt cgaaggcgaa
     4741 tcgatgcatg gttgcggcga tggtcgccgg cgccggcaac aatttgtcgc cgggattcgc
     4801 ggccagtcgt gcgtgcgagg caaacagata ggcggcaatc aagacgataa acggcaaagt
     4861 gctcaagccg agcgcgccgc ctcgtcctgg gtgctgattg atgagccgtc gcacgggtca
     4921 gcgcctcaca atttcccgtc ggcggccagc ttggtgaaac tcgaatcgaa gcgcattttc
     4981 aaattgtgca ggtcacccaa ggtcttgccg gctgggaatt gaatgccgac gacgtcggcg
     5041 gagctcgcgc cttgccctaa gattccgtgt tcgaaggaaa acttgcgcac caggtccatg
     5101 ctcttgatga cgccgtcggc cgtgacgaag gcgtaggcgt cggccgctgt cgcgaacacg
     5161 tgcgtggtcg cgagctgcag cccgaagccg gccaaatccg taccggatgc ctgggccatg
     5221 gctgtctgcg cagcgcggcc cgcggcgtca ttcctgaaca ttacccccag agtttcgtac
     5281 caggcgccga gcagcgcttt gccgagcttc ggattggcat tgaggacatc gctcttgacc
     5341 accatcaggt cgagaatttc gccgggaatt ttgctcgagt caaacaccag ctgcgcattc
     5401 ggctgagcca acaccgcact cagctgcggc ttccacgtga ccaccgcggt ggtcgccggc
     5461 gcgctgaagg ccgcgacgat atcggcatcg gaggtgttga tgatcttcaa gtcgcgttcg
     5521 cgcagtcccg cggagctgag cgcccgtacc agcaagtaat gcgacacgga caattccacg
     5581 agattgacct tctggccttt gatgtcgccg agcgtcttgc ccttgccctt gagcaccacg
     5641 ccatcgttgc cgttggaaaa atcgccgacg atgagcgcgg tcgaatcgac gccgcccgcg
     5701 gccggaatcg tcagcatgtc catattcgtc atgacacagc cgtcgaaact gccggcggtg
     5761 tactgattga tcgactccac gtattcgttg acttgcgtca atttgatctt gatcccgtac
     5821 ttatccgccc acttcttcaa gataccgctg tgatccgcgt agtcccatgg catccagccg
     5881 acatagatgc tccaggcgat attgaaagag ctgcgttcgg ccgccgctgc cggcaatgcg
     5941 ctgccgacgg ctaagattcc caacagcggg atcagaagca ggtctttgag gcgagtgagc
     6001 atcgaatctc tccttacact gaatacgtca ggcagctgcg cgctcatgcg ggcgcatgac
     6061 taccaaagtt tgcgcgatca gcacggcgcg cccttccacg cagcgcattt ggatcaccac
     6121 cccatccagc ggcgcggtga ccggcaattc cattttcatc gactcaacca acagcagtgt
     6181 gtccccggcc ttcacggatt gaccgggcgc cgaggaaatc ttccaaacgc tacccgccac
     6241 cggactcaac acagcgacgc atccgggcgg caactcctcg ccgtccgctt cttgagcggc
     6301 cggaagatcc gccgcatagc cgatctgtcc ggttgcctcc cagcgcctgc gctcggagtc
     6361 gaaagcggac tgttggcgca tcttatgggc ggcgatgctg gcggcgtttg cggctaagaa
     6421 acttcgatac tcgttgagct ggaatgtcgc gtcgcgaatg tcgaggcgcg cgcgcccgtg
     6481 gagaaagtcc gcacggaatt ccttgagttc ggcgccgctc acgggaaaga agcgaatctg
     6541 atcgaagaat cgcagcaacc agggtttgcc gtgcgtgaat tccgcggtga ccttgtaggt
     6601 gttccacacc tggcacgtgc gaccgacgaa ctgatagccc cccggtcctt ccatgccgta
     6661 aatgcacaaa taggcgccgc cgataccgac ggcgttctcc ggcgtccagg tgcgcgccgg
     6721 gttgtacttg gtggtcacca gtcggtgccg tgggtcgagc ggcgtcgtca ccggcgcgcc
     6781 caagtacaca tcgccgagtc caaggaccaa gtaattggcg tcgaacacga tgcgcttcac
     6841 ttcctcgacg ctgtcgagac cgttgactcg acgaatgaat tcgatattgc tcgggcacca
     6901 gggcgcatcg gcacgcaccg attgcatgta cttggcgatg gccagttggg tggactcgtc
     6961 gtcccaggag agcggcagat tcacgatgcg ggtggcgatc tgccgatcgg cggtcgcggc
     7021 cagcgcgcgc tcgccggcgc gcagcacatc gagcagcgca gcgagcggca gaacgcggtc
     7081 gtcgtaatgc agctgcagcg agcggatccc gggagtcagg tcaatgatgc ccggcggagc
     7141 cgttgtctgc agccacgaca tcagcgcgtg tacgcgcagg cgcaattcca gatcgaggac
     7201 cagcgcgccg tattcgacga gcacgtagcg gtcgccggcg cgccggtaaa cgacggatgg
     7261 ccgcgactct cgcgccgcga actgcgcgag tatcggcgtg tcggcggccg catcggcgcg
     7321 cttcatgcgc gatgtcgacg cacgcggcct cgccaacgtc tcgaccagcg actgttgcag
     7381 agcttcggcc tgttgcgcct cctcgagaga caggcgcttg aatcgcacgc ggtcattcgc
     7441 ccgcagctgg ccgagtttcc atagctcggc gccaaccacc gtcaccggac agacgaaacc
     7501 gccgagcgag gggccatcgg gtcccaagat gaccggcatg tcgccggtaa aatccaccgt
     7561 gccgatggcg taggcgttgt catgaatgtt cgacggatgt agtcccgcct caccgccgtc
     7621 gctgcgtgcc cacttcggtt tcggtccgat cagccgtaca ccggtgcggc tcgagttgta
     7681 atggacctcc caggttgcgg caaagaattc ggcgatgtcc gcgtcggtaa agaaatccgg
     7741 cgcgccgtgc ggaccgtaca gaacgccgag ttcccaatca ttaccaatgg cgggcatcaa
     7801 gtcgctggga atcgcctgcg gttgcttctc atgggcgccg gcctgtgtgc gcgctggacc
     7861 gacgtgcaac acatcgccca ccatcagcgc gcggccgccg tggccgccga atttaccaag
     7921 agtaaaggtc gagcggctgc ccaagtagag cggaacgtcg aagccgccgc ggatcgcgag
     7981 gtagctgcgg caacctgcgc cttgaatggc acttaaggtc agcgtctcgc cgcggcgcac
     8041 cgcgagcgga gttccgaact cggccgttcg acctgcgaga ttcgccgcca tcgcggcacc
     8101 gaccagacaa atcgtggcgt cggagttgaa tcttagagtc gctcccgaca cggtcatttc
     8161 gagcgccgcc gcccgcggtg aattgccgag agcgcgattg gcgagacgga atgacaggct
     8221 atccatcggt ccggacggcg gcacaccgac gttccaaaaa cccaatctgc ccggataatc
     8281 ctggatggtc gtttgcgtgc cggcggcgag cacttccacg gtgttcgcag cgtaggcgaa
     8341 gctctcgagc gcgcgggtgg tcatccggcc gccggcgaat acgggatccg cgaccagttg
     8401 ccgcaagtag tcgaggttgc tctcgatacc gccgatgtcg gtcgccgcca gtgctgcgcg
     8461 catcgccgac aacgcggcgg ctcgatccgc acccacggtg atgatttttg cgagcaacgg
     8521 atcataaaac ggagtgacca cagagccgtc ttcgatccag gtctcgatgc gcgcattcgc
     8581 tgcaaatcgg acgcggctca aaagaccggt gctcggcctg aattgccgcg ccggatcttc
     8641 ggcgtacagc cgtgcttgga ttgacgcgcc ccgcgcctgc ggcgtccgca acgcaaaatc
     8701 ctcatccgcc gcctgacgca ccatccactc gaccagatcg acgccggtga cctcttcggt
     8761 cacgccatgc tcgacctgca gccgcgtgt
//
LOCUS       DASWZT010000577         4560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_62812, whole genome shotgun sequence.
ACCESSION   DASWZT010000577 DASWZT010000000
VERSION     DASWZT010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62812"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="VGE92_16515"
     CDS             complement(<1..1317)
                     /locus_tag="VGE92_16515"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY1315488.1"
                     /translation="MRLAGDYDNIIVGAGTAGCILANRLTADPRRRVLLLEAGGRDNW
                     IWYHVPAGYLFAIGNPRADWMFRTQSEPGLHGRSLAYPRGKVLGGSSAINAMICMRGQ
                     AADYDGWRALGLPGWGWNDVLPFFKRLEDHFLGASEHHGIGGGFRVEAPRVRWRVLDA
                     VREAAVQMGVPSVEDFNCGDNEGVSYFHVNQKRGRRWSSARGFLQPALARSNLRLELH
                     AVVERLLIETGRVCGVQFRRDGESCEARTGGEVILAAGAIGSPHILSLSGIGRQEWLS
                     PLGIRSHVDRPGVGSNLQDHLQQRAIYKLRGVKTLNEAYHSVLGRAWMGIDYLVRRRG
                     PLTMAPSQLGIFMRSDPAQPRANIEFHVQPLSLDKFGDPLHAFSAITVSACNLRPTSR
                     GTVRPASADPSAPPLIAPNYLSTAQDRIVAADAIRATRRLMSQAAVA"
     gene            complement(1386..2744)
                     /locus_tag="VGE92_16520"
     CDS             complement(1386..2744)
                     /locus_tag="VGE92_16520"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1315489.1"
                     /translation="MHPRATLAATILGSSLAFIDGSVVNVALPALARDLRVDPANLSW
                     AINAYLLPMGVLMLLGGALGDHFGRRRLFQTGLALFSIASAVCAAAPSLGWLLAARAL
                     QGLGAALLMPNSLAILGTGFFGEARGRAIGTWAAAGALAGAVGPVAGGWVVDVIGWRS
                     IFLLNLPLAAGAAYLAWNYVAEGMESQPSALDVLGAALATLGLGSIIWALTKAADSSA
                     SRSSVWPSALAGIILIGAFLWQEQRLGERAMMPLALFAAPTFVGLNLLTFFLYGSVGG
                     LMVLLPFFLIAKEHWLATAAGAALLPLPILIGFGSRFMGHVASRYGERLPLTLGCSLV
                     AMGLALFTRIRIDEVHYWRDIFPAMLFVALGMGACVAPLTTSVMTSVQAQRVGLASGF
                     NSAVARVAGLIATALLGFVFARQASAQTFLGAFHAAALIGAASAALAAGSAFLLVKSK
                     TS"
     gene            complement(2756..>4560)
                     /gene="ligD"
                     /locus_tag="VGE92_16525"
     CDS             complement(2756..>4560)
                     /gene="ligD"
                     /locus_tag="VGE92_16525"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEY1315490.1"
                     /translation="TGLDWTDRFAAIAADARALPDCIIDGEVVALDAHGLPSFSALQA
                     ALSEQRSQELVLFAFDLLFKGREDLRPRPLAERKTELEKLLNEPQFNQRLRYVAHLQS
                     RAQDVYASACKMGLEGIVSKQLDAPYRSGRSGSWTKIKCREGQEVVLGGWTTEAGTVR
                     SLLAGVYRGEEFVYVGRIGTGFGRKVANLLLPRLKQLTRTSSPFQGHTAPPAEKNIRW
                     LKPSLVAEIQFAGWTATHMIRQAAFKGLREDKAARDVVAEVATDPIDEGPIEGKSPMI
                     AKPAAANKRTNRLPSPPKSPGSMSIMGVSISKPDKALWPDAGDGTPVTKADLAQYFAH
                     VGAWMLPHLAGRPCSLVRVPDGLGTQQFFQRHVMPGMSPQLTSVKIKGDKAPYVQIDR
                     VEGLIAAAQVAVLEIHPWNCAPDDPEFAGRLVFDLDPAPDVTFSAVIAGAIEIRDRLK
                     ASGLESFCKTTGGKGLHVVTPLRTGKAAVRWPVAKNFAHIVCAQMAQDSPAKYLDNMS
                     KRRRVGKIFLDYLRNDRTATAVAVLSPRAREGAPVSMPIDWQQVRAGLDPKKFTVRTA
                     AALLQKSRPWAGYDAASRSLAEAIRMLTQKAKPG"
BASE COUNT          913 a         1464 c         1428 g          755 t
ORIGIN      
        1 cgcgacggcc gcctggctca tcaggcggcg cgtcgcgcgg atggcatcgg ctgccacgat
       61 tcgatcttgc gcagtcgata gataattcgg cgcaatgagc ggcggcgccg acggatcggc
      121 ggatgcgggg cgcaccgtgc cgcgcgaggt agggcgcaaa ttacaggcgc tcaccgtgat
      181 cgccgaaaat gcatgcaatg ggtcgccgaa cttgtccaag gagagaggct gcacgtggaa
      241 ctcgatattc gcgcgcggtt gagctggatc ggagcgcata aatataccaa gttgcgaggg
      301 agccatggtg agcggcccac gacggcgcac gagatagtcg atgcccatcc acgcgcgtcc
      361 aaggacagag tggtaggcct cattcaaagt cttaactcct ctgagtttgt agattgcgcg
      421 ctgttgcagg tgatcttgca gattggatcc gaccccgggc cggtcgacgt gcgaccggat
      481 gccgagcggt gacagccact cctgcctgcc aatcccggat agactgagga tgtgcggcga
      541 gccgatcgca ccggccgcaa gaatgacctc gccgccggtt cgcgcttcgc atgactcacc
      601 gtcgcgcctg aactgcacgc cgcagacgcg cccagtctcg atcagtagcc gctcgacgac
      661 ggcgtgcagc tcgagtctca gattgctccg tgcgagggcg ggctgcaaga agccgcgcgc
      721 cgatgaccag cgacggccgc gcttttgatt gacgtgaaag taggaaactc cctcgttgtc
      781 gccacaattg aagtcctcga ccgagggcac gcccatctgc acggctgcct cgcggaccgc
      841 atcgaggacc cgccatctaa cccggggcgc ttcaacccga aatccgccgc cgattccgtg
      901 gtgctcgctc gcgccgagga aatggtcttc cagacgcttg aagaatggca acacatcgtt
      961 ccagccccag cctgggagac cgagggctcg ccagccgtca tagtccgccg cctggcctcg
     1021 catgcagatc atggcgttga tcgcagagga accgcccagg actttgccgc gcggataggc
     1081 aagcgagcgg ccgtgcagtc ccggctcgct ctgcgtgcgg aacatccaat cggcgcgtgg
     1141 attgcctatg gcaaacaggt agccggcggg gacgtgatac caaatccaat tatcccggcc
     1201 gccggcctcg agcagcagca cccggcgtcg cggatcggcc gtcagacggt tggccaggat
     1261 acagcccgcc gtgcctgcac cgacgatgat gttgtcgtaa tcccccgcca gtcgcatcac
     1321 ctcatcctcg catgaataga tgagttttac ctgagcgcgt accggctacg gcgtgacgaa
     1381 gtcgctcaag acgtcttgga cttgacgagc aggaacgcgg atccggcggc taatgctgcc
     1441 gaggctgcgc caatcagcgc cgcagcatga aacgcgccga gaaaggtctg cgctgacgcc
     1501 tgacgcgcga acacaaagcc cagcaaggcg gtggcgatca gcccggcgac gcgcgccact
     1561 gccgagttga agcctgacgc caacccgacg cgctgcgctt gcaccgaggt catcactgag
     1621 gtggtcaggg gtgcgacgca ggcgcccatg ccgagggcga cgaacagcat tgcaggaaaa
     1681 atatcgcgcc aatagtgcac ctcgtcgata cggatcctcg tgaacagagc cagacccatt
     1741 gccacgagac tgcagcccag ggtcaacggt agccgttcgc cgtagcgcga ggccacgtgc
     1801 cccatgaatc gcgatccgaa gccgatcagg atgggcagcg gcagcagcgc cgcacccgcg
     1861 gcagtcgcca accagtgttc ctttgcaatc aggaagaatg gcagcaggac catgagtccg
     1921 ccgacactgc cgtaaagaaa gaatgtcaat aaattgagac ccacaaacgt gggcgctgca
     1981 aatagagcga gcggcatcat cgcgcgctcg cccaagcgct gctcttgcca aagaaatgcg
     2041 ccgatgagaa tgatgccggc aagcgcggac ggccagaccg agctgcgcga cgcgcttgaa
     2101 tccgcggctt tggtgagcgc ccagataatc gaaccgagtc cgagcgtcgc gagtgcggcg
     2161 ccgagcacat cgagcgctga gggctgcgat tccatgccct ccgccacgta gttccacgcc
     2221 aggtacgcgg cgcccgcagc gagcggcaaa ttcagcagga aaatcgaccg ccacccgatc
     2281 acatcgacca cccagccccc tgcgacgggt cccacggcgc cggccagggc accggccgcg
     2341 gcccaggtcc cgatggcgcg accgcgtgcc tcgccgaaaa aaccggtgcc gaggatggcg
     2401 aggctgttgg gcatgagcaa cgcggctccg agtccttgca gagcacgcgc cgcgagcaac
     2461 caaccgagcg agggagcggc cgcacacacg gccgatgcga tcgagaacag cgcgagtccg
     2521 gtttgaaaca gccggcgccg tccgaaatga tctccgagtg cgccgccgag cagcatcaag
     2581 acacccatcg gcaagaggta ggcgttgatc gcccacgaca aattggccgg atcgacccgc
     2641 agatcccgcg cgagagcggg cagggcgaca ttcacaaccg agccatcgat gaacgccagg
     2701 ctcgatccga gaatggtcgc cgccaatgtc gcacggggat gcatgtgaaa cgagcctagc
     2761 ccggcttggc cttctgcgtg agcatgcgaa tcgcctcggc gagcgagcgc gacgcagcat
     2821 cgtatccggc ccacggccgg ctcttctgca gcaaagcggc agcggttctc acggtgaact
     2881 ttttcggatc gagacccgcg cgaacctgtt gccaatcgat gggcatcgag accggcgcac
     2941 cttcccgtgc gcgcggcgag agtacggcca ccgccgtcgc ggtgcgatcg ttgcgcagat
     3001 aatccaaaaa tatctttcca acgcgccggc gcttcgacat attgtccagg tatttggcgg
     3061 ggctgtcctg cgccatttgc gcgcaaacga tgtgtgcaaa attcttggct acaggccagc
     3121 gaaccgctgc cttgcccgtg cgcagcggcg tgaccacgtg caggcctttg ccgcccgtgg
     3181 tcttgcagaa ggattcgagg ccgctcgcct tgaggcgatc gcgaatctcg atcgcgccgg
     3241 cgatgacggc gctgaatgtt acgtcgggtg cgggatcgag gtcgaacacg agtcggcccg
     3301 cgaattccgg atcgtcagga gcgcaattcc acggatgaat ctcgaggacg gcgacctgtg
     3361 cggcggcgat caagccttcg actcgatcga tctggacgta gggagccttg tcgcccttga
     3421 tctttacgga agtgagctgc ggcgacatgc ctggcatgac atggcgctga aagaattgct
     3481 gcgtgccgag accatcgggc acgcgcacca acgagcaggg gcggccggcc agatgcggca
     3541 acatccaagc cccgacgtgc gcgaagtatt gcgcaagatc ggccttcgtc accggcgtcc
     3601 catcgcctgc gtccggccat agcgctttat caggtttgga tatcgacacg cccatgatgc
     3661 tcatcgagcc gggactcttg ggcggggagg gcagtcgatt cgtgcgcttg ttggcggcgg
     3721 ctggtttagc gatcatcggt gatttacctt cgatagggcc ctcgtcgatg ggatctgtcg
     3781 caacctccgc cacaacatcc cgcgccgcct tgtcttcgcg caagcccttg aacgcggcct
     3841 gacgaatcat atgcgtcgcg gtccagcccg cgaactgaat ttcggcgacc aggctcggct
     3901 tcagccagcg aatatttttc tccgcgggag gcgccgtgtg gccttggaac ggactgctcg
     3961 tgcgcgtcaa ctgtttcagg cgtggcagca ggaggtttgc gaccttgcgc ccgaatccgg
     4021 ttccgatccg gccaacatac acgaactcct cgccgcgata gacaccggcc aacagcgagc
     4081 gaaccgtacc cgcttcggtg gtccagccgc cgagcacgac ctcttgtcct tcgcgacatt
     4141 tgatcttggt ccagctgccg gatctccccg agcggtacgg ggcgtccaac tgtttcgaga
     4201 cgatgccttc gagtcccatc ttgcaggcgg acgcatagac atcttgcgcc ctcgattgca
     4261 aatgcgcgac atagcgcaag cgttgattga actgcggttc attcaacagt ttctcgagct
     4321 ccgtcttgcg ttccgccagc ggccgcgggc gcaggtcctc ccgccctttg aacaataaat
     4381 caaacgcaaa caacacgagc tcttgcgacc tctgctccga cagggcggcc tgcagcgcgc
     4441 taaaactggg taagccgtgc gcatccaggg cgaccacctc gccgtcgatg atgcagtcgg
     4501 gaagcgcgcg cgcatccgcg gcaatcgccg caaagcgatc ggtccagtca aggccggtgc
//
LOCUS       DASWZT010000578         4250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_57945, whole genome shotgun sequence.
ACCESSION   DASWZT010000578 DASWZT010000000
VERSION     DASWZT010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57945"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VGE92_16530"
     CDS             <1..595
                     /locus_tag="VGE92_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315491.1"
                     /translation="FVGMRAFRAAQRRFGWWDAFRRSKDPPTFIVVFEDSAAILGIGA
                     AAAGAAAAIYTGDSRWDGIASLGIAVILAAVAALLARESKELLIGERADPALSDAIMR
                     TASGIPGVCSANSIVTVQLAPANVIATLSLDFFDYLRAPDIERAVLELEARVRSAHPE
                     VRALFVKPQSVLVAAQRSQDGSAGMTADSLEIEIGDG"
     gene            complement(605..1459)
                     /locus_tag="VGE92_16535"
     CDS             complement(605..1459)
                     /locus_tag="VGE92_16535"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEY1315492.1"
                     /translation="MQYKDYYEVLGVTRGADAEAVKRAYRKLARKFHPDVSKEKNAEN
                     KFKEVQEAYEVLRDPEKRAAYDQLGRDYRPGQQFRPPPDWSQRFGHSGSQRFSDLNGF
                     SDFFSSLFGGGADAPPHEADAGRLDVTLEEAFAGTKRRVMLNEGGRSRSVDVQVPPGV
                     SEGQSLRIPGTGGHASLIFRIRVRPHSLYALQGKDVQVELPLAPWEAALGAKVAVPTL
                     GGTVEVTVPAGAQSGQKLRLRGRGFPGGDQLVVIKIVTPAASSAQARQAYERMRSEFD
                     FNPRAGWP"
     gene            1643..2524
                     /gene="htpX"
                     /locus_tag="VGE92_16540"
     CDS             1643..2524
                     /gene="htpX"
                     /locus_tag="VGE92_16540"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEY1315493.1"
                     /translation="MKRIFLFLVTNLAVLALLSLVIFVIEQVFGVRLGGGGLGGLLVF
                     AAVFGFGGALISLALSKWTAKRMMGVRVITAPQSDLESWLLGTVKGLADKAGIGMPEV
                     GIFDAQEMNAFATGARRNAALVAVSSGLLRGMTRTQAEAVLGHEITHVANGDMVTLAL
                     LQGVLNTFVIFLARIIGSFVDRALFKNERGESGIGYFLTTMAAQLVLGVLASMIVSWY
                     SRRREFRADRGGGNLAGTGNMIAALEVLKRSQGEPMPPQMQAFGINTGSTSGFMQLFM
                     SHPPLDERIAALQKPAA"
     gene            2571..3554
                     /locus_tag="VGE92_16545"
     CDS             2571..3554
                     /locus_tag="VGE92_16545"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEY1315494.1"
                     /translation="MTPENEIVVEPPAVERVSLELSRWFGNSDFATRPADLVVPRESG
                     MHVDWARVVPFLLMHAACLAVIWVGFSWVALSVTLLLYVLRMFAITGFYHRYFSHRSF
                     KTSRVGQFIFGLLGASAVQRGPIWWAAHHRHHHIYSDKPEDVHSPVQHGFFWSHMGWF
                     MSHKHFAADLSRVKDLLKYPELRFLDRFDIAVPTALGVGVFLLGVLLKHVAPGLGTNG
                     WQMLVWGFFVSTVAVYHGTYTINSLSHVFGRQRYKTGDASRNNVWLAIITLGEGWHNN
                     HHHYPASVRQGFYWWEFDITFYMLKLMSFVGVIWDLKPVPAAVREGAGKRF"
     gene            3638..>4250
                     /locus_tag="VGE92_16550"
     CDS             3638..>4250
                     /locus_tag="VGE92_16550"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1315495.1"
                     /translation="MRIAVIGSGISGMVAAYHLSRSHEVTMYEAGPHIGGHTNTVDVE
                     YQGRDYAVDTGFIVYNDWTYPNFIRLLDTLGVPWQPSQMSFSVRCERSGLEYNGTSLN
                     SLFAQRRNLVRPAFLRMVADILRFNQRAPRLLESPRDGVTLSDYLEREAYSKYFIDHY
                     IIPMGAAIWSSRPVDMLHFPARFFVEFFANHGFLSVDDRPTWRG"
BASE COUNT          750 a         1319 c         1307 g          874 t
ORIGIN      
        1 gttcgtagga atgcgggcgt tccgcgcggc gcagcgccgc ttcggctggt gggatgcgtt
       61 tcgccgctcg aaggatccgc ccactttcat cgtcgttttc gaagattccg cggcaattct
      121 cggtatcggt gcggcggcgg ccggcgcggc cgcggcgatc tacaccgggg attcgcgctg
      181 ggacggcata gcctccctgg ggattgccgt gatcctggcg gcggtcgcag ccttgctcgc
      241 cagagaatcc aaggaacttc tgatcggcga acgcgccgac ccggcgctca gcgacgccat
      301 catgcgcacc gcgtccggca tcccgggcgt gtgcagcgcc aacagcatcg tcacggtgca
      361 attggcgccc gccaacgtga tcgcgacctt gagcctagat ttcttcgatt acctgcgtgc
      421 gccggatatc gagcgcgcgg tgctcgaatt ggaggcgcgt gtccgcagcg cgcaccccga
      481 agtgcgggcg ctgttcgtca aaccgcaaag cgtactggtg gcggcgcagc gatcgcagga
      541 tggcagcgcc ggcatgactg cggacagtct cgagatcgaa atcggcgacg gttgaggcct
      601 taggttacgg ccagccggcg cgcggattga aatcgaattc gctgcgcatg cgttcgtaag
      661 cttgcctcgc ctgcgcggat gacgctgcgg gtgtcacgat cttgatgacg acgagttgat
      721 cgccgccggg aaaaccgcgc ccgcgcaatc gcagtttctg gccggattgg gctcctgcgg
      781 gcaccgtcac ttcgaccgtc ccgccaagcg tcggtacggc cacctttgca cccaaagcag
      841 cctcccaagg agccaaaggc aactcgactt gaacgtcctt gccttgcaac gcgtagagcg
      901 aatggggacg aactctgatg cgaaatatca gcgacgcatg gccgccggtc ccgggaatgc
      961 gcagcgactg cccctcgctc actcccggcg gcacctggac gtcgacagag cgcgagcggc
     1021 cgccttcgtt cagcatgacg cggcgcttgg tgcccgcaaa cgcttcttcc aaggtgacgt
     1081 ccaagcggcc cgcatcggcc tcgtgcggcg gggcatcggc accgcctcca aacaggctcg
     1141 agaaaaagtc gctgaacccg ttgagatcgg aaaaccgctg gctgccggag tgaccgaatc
     1201 gctgcgacca atcgggcggc ggcctgaatt gctggcccgg ccgataatcc cggcccaact
     1261 gatcgtaggc cgcacgcttc tccggatcgc gcagcacctc gtaggcttcc tggacctctt
     1321 tgaacttgtt ctcggcgttc ttttccttgc tgacgtcggg atggaacttc ctggcgagct
     1381 tgcgataggc gcgcttcacc gcttccgcgt cggcgccgcg ggttacaccc agaacctcgt
     1441 aataatcctt atattgcatt cctaactatc cggtattttc catatattac caatacatag
     1501 ccgcgcttgt ccgtccagcg gccgtgcgcg gcgtcgcccc cgggccgcaa gcgtgcctgt
     1561 catccttgaa atcgacccca acgtcccgac cattactccg tcgcgttagc atattgcggt
     1621 tggattcagc aaagaaaagc ccatgaagcg cattttcctg tttctagtga cgaacttagc
     1681 cgttctggcc ctgctgagct tggtgatttt cgtcatcgag caggtgttcg gcgtgcgcct
     1741 tggcggcggc ggactgggcg gcctgctggt atttgccgca gttttcggct ttggcggtgc
     1801 gttgatctcc ctcgcgctct ccaaatggac ggccaaacgc atgatgggcg tgcgcgtcat
     1861 cacggcgccg caatcggatc tcgaaagctg gttgctcggc acggtcaaag gtttggcgga
     1921 taaggcagga atcggcatgc cggaggtcgg catattcgac gcgcaggaga tgaacgcgtt
     1981 tgcgaccggc gcgcgtcgca acgcggccct ggtcgcggtc agcagcgggc tgctgcgcgg
     2041 catgactcgc acccaggccg aagcggtatt gggtcacgaa attacccatg tcgcgaatgg
     2101 tgatatggtt acccttgcgc tgctgcaagg tgtgctaaac acgtttgtga tatttctcgc
     2161 tcgaatcatc ggcagctttg tcgatcgggc gctgttcaag aacgagcggg gagaatccgg
     2221 aatcggatat ttcctcacca cgatggccgc gcaactcgtg ctcggcgtac tggccagcat
     2281 gatcgtgagt tggtattcgc gcaggcggga gtttcgcgcc gaccggggcg gtggcaatct
     2341 tgccggcaca ggtaatatga tcgctgcgct cgaggtattg aagcgcagcc aaggggagcc
     2401 gatgccaccg caaatgcagg ccttcggaat caacaccggc agtacgagcg gcttcatgca
     2461 gctgttcatg agccatccgc cgctcgacga acgcatcgcg gcactacaaa agccggctgc
     2521 ataggcatcc gcggcggcag gtcgaccgta gacactcaat aggacacgta atgaccccag
     2581 aaaatgagat cgtggtagag cccccggccg tcgagcgcgt cagcctggaa ctgtcgcgct
     2641 ggttcggcaa tagcgacttc gctacccgtc cggccgacct ggtggtgcct cgagagtcgg
     2701 gcatgcatgt cgactgggcg cgggtcgttc ccttcctgct gatgcatgcc gcctgtctcg
     2761 ccgtgatttg ggtgggcttc agctgggtgg cgctgtcggt tacgctgctg ctctacgtac
     2821 tgcgcatgtt cgccatcacc ggcttttatc accgctattt ttcccaccgc agcttcaaga
     2881 cctcgcgggt cggccaattc atattcggct tgctgggcgc ctcggcggtg cagcgcggcc
     2941 cgatatggtg ggccgcacac catcggcacc accacatcta ctccgacaag ccggaggacg
     3001 ttcattcacc cgtgcagcat ggcttcttct ggagccatat gggttggttc atgtcgcaca
     3061 agcacttcgc ggccgatctg tcacgcgtga aggatctgct caagtatccg gaactgcgct
     3121 tcctggatcg cttcgatatc gcggtgccga cggcgctcgg cgtcggcgtg ttcctgttgg
     3181 gggtgctgct caagcatgtc gcgcccggcc ttggaaccaa cggctggcag atgctggtgt
     3241 ggggattctt tgtctccacg gtagcggtct accacggtac ttacaccatt aattccctgt
     3301 cccatgtctt cggtcgtcag cgctacaaga ccggtgatgc cagccgcaac aatgtgtggc
     3361 tggcaatcat cactctcggg gagggttggc acaacaatca tcaccactac cccgcgtcgg
     3421 tgcgccaagg gttctattgg tgggagttcg acatcacctt ttacatgttg aagctgatgt
     3481 cgttcgtcgg ggtgatttgg gacctcaaac cggtgccggc ggccgttcgg gagggggccg
     3541 gcaagcgatt ttaaccatca gcgcatccat tgcgtcgtcc gggtcgaatg atgctcggta
     3601 agccggataa gccggtgacg aggagtgacc ccaggcaatg cgtatcgcgg ttatcggttc
     3661 cggcatttcc ggcatggtgg cggcctacca cctgagccgc tcccacgaag taaccatgta
     3721 tgaggcgggc ccccacatcg gcggtcacac caataccgtc gacgttgaat accaggggcg
     3781 cgactacgcg gtcgacaccg gctttatcgt ctataacgat tggacctacc cgaatttcat
     3841 ccgcctgctc gacacgctcg gggtgccgtg gcagccgagt cagatgagct tcagcgtgcg
     3901 ctgcgagcgc agcgggctcg agtacaacgg caccagcctc aattccctgt tcgcccagcg
     3961 ccgcaacctg gtgcggccgg cgtttttgcg catggtggcc gacatactgc gcttcaatca
     4021 acgcgcgccc cgcctgctcg agtcgccgcg ggacggcgtg accttgagcg attacctcga
     4081 gcgcgaggcg tactcgaaat acttcatcga tcactacatc atccccatgg gcgccgccat
     4141 ctggtcgtca cgtccggtcg acatgctgca cttccccgcc cgattcttcg tcgaattctt
     4201 cgccaaccat ggattcctga gcgtcgatga ccggccgacg tggcggggaa
//
LOCUS       DASWZT010000579         9841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_72523, whole genome shotgun sequence.
ACCESSION   DASWZT010000579 DASWZT010000000
VERSION     DASWZT010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9841
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72523"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1784)
                     /locus_tag="VGE92_16555"
     CDS             complement(<1..1784)
                     /locus_tag="VGE92_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY1315496.1"
                     /translation="MLSQELEFCLNDAFAGAREARHEFMTVEHLLLAIVDTPKVREVL
                     KSCGADTAKLKTELKQFIDQTTPRLPAGEDRDVQPTLGFQRVLQRAVFHVQSSGKKEV
                     TVANVLVAIFSEKQSHAAYLLSMQDVSRLDVVNFIAHGLSKAGGEERSEREDGGASPD
                     ADRENDGGGNALEKYATNLNESARKGKIDPLIGRKLEVERTIEILCRRRKNNPLYVGE
                     AGVGKTAIAEGLARMIVENQVPDVLLDATIYSLDMGSLVAGTKYRGDFEKRLKAVLAE
                     VKKQPGAILFIDEIHTVIGAGAASGGVMDASNLIKPALANGDLRCIGSTTYNEYRGIF
                     EKDHALARRFQKIDITEPSVQDTVDILRGLKSRYEEHHGIKYEDDALRAAAELAARHI
                     NDRHMPDKAIDVVDEAGANLRLQPVEHRALSVTVTQIENVVARIARIPPKNVSLSDRD
                     VLKNLERNLKLTIFGQDKAIEALSSAIKMSRSGLGDQRKPVGSFLFSGPTGVGKTEVT
                     RQLAIAMGVEFVRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTEAIAKHPHCVLLL
                     DEIEKAHPDVFNLLLQVMDHGTLTDNNGR"
     gene            complement(1787..2107)
                     /gene="clpS"
                     /locus_tag="VGE92_16560"
     CDS             complement(1787..2107)
                     /gene="clpS"
                     /locus_tag="VGE92_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004646784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEY1315497.1"
                     /translation="MSDIERGRDGTGLVVEEAAPKLKRPPLYQVVLINDDYTPMEFVV
                     DILQRFFTMDRTKATRVMLEVHTKGKGVCGVFTYEIAETKVAQVIAYARQHQHPLMAT
                     LEEA"
     gene            2253..3269
                     /gene="gspC"
                     /locus_tag="VGE92_16565"
     CDS             2253..3269
                     /gene="gspC"
                     /locus_tag="VGE92_16565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HEY1315498.1"
                     /translation="MPITVTIRDPYMLDTRSNEWLSRLQGNGPRLVSLALAALIAVEL
                     ARIAISVLSGGPVKSPQPVLTSAAPRGLHAGVDVQAVTSAHLFGVAVIDPAAQDPANA
                     PQSTANLVLAGTIATQDPKHGVAIISDGGPSKVYSVGDNIGGASLHSVYLDHVILDRV
                     GVLETLPLPRLLGPGMHAAPIVRRTGGDPRTVAAVDNIRRMVQQDPGILDQVMRTVPS
                     YDNAAGKLRGFRAYPGRNRLIFTKLGLRSGDLVTAINGTQLDDPQRSQEVFNTIQTSD
                     RATVTIERNGQKQDITLNIAQVAAQATKDLEGDNTGSGAPANGAPQPPPSGAPNSPNE
                     NPNE"
     gene            3310..5511
                     /gene="gspD"
                     /locus_tag="VGE92_16570"
     CDS             3310..5511
                     /gene="gspD"
                     /locus_tag="VGE92_16570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HEY1315499.1"
                     /translation="MMPNSNNPQTKTRIAAVLALAAALLVSAASPAAPPAKPQQDTPT
                     ITPNYKDADLSQIIQAVAEVTGKNFIIDPRVNAKVTMLSATPMSPAAFYEAFLSVLQV
                     YGYVAVPAGKVIKIVPNTDARQLPANDLPASVSSSSDEIVTQIITVKNISAAQLVPIL
                     RPFVPQYGQLTAYPSGNMLIISDRASNVSRIVRIVERMDSSEDEPIEVIALHNASASD
                     VVRVVNSINQGGQGAEGAGSAVKIVADDRTNSILLTGEKSQRLKLKALIVNMDTPLAT
                     GGDTQVRYLRYADAEKIADKLKGQATATAKAQGGPPAAGAAGGGGGGTANVDSSVTIW
                     ADVATNALIITAPPKIMKSLMNVIDKLDIRRAQVQVEAIIVEVDVNKSSNIGVQWLLD
                     GGNNYGYGVTNLPGSGTSIVDLAAAVLGVSQGTATSAVTTGTAGTTTTSGLSSSTVGT
                     GATFAIGKYNQNTGKGFAALIQALRSDGSSNIISTPSLITMNNEEAEVKVTQEIPLIT
                     GSYSSSTASVGGTTSPFTTIQREEVGTILKVTPHINEGNSVQLKIEQEDSSPGAKLTD
                     SADISTNKRSIKTTVLIEDGGIIVLGGLMSDTVTESEDRVPGLGAIPLLGNLFKSRSG
                     SRQKKNLLVFIRPKILRDADATESTSESKYNEVRQDQRTLNNGHITLLPGQKQPVVPP
                     MPPGSGLPAPPPSGANAQGATPNMPPPSEPAPQTTPVPTPAPAPSNPPPNP"
     gene            5508..6998
                     /gene="gspE"
                     /locus_tag="VGE92_16575"
     CDS             5508..6998
                     /gene="gspE"
                     /locus_tag="VGE92_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HEY1315500.1"
                     /translation="MNAELNDPVEQRKLSFPFAKRHGVLVRGIAEGVADTVCRPGVTP
                     QSLAEVRRFVGVPLKLQRVSSEAFDSLLRVAYEAGSGAAMQMLEGLDDNTDLAHLAED
                     IPESSDLLESDDEAPIIRLINALLTQAVKENASDIHIEPYENRLVIRFRVDGVLREVL
                     QTKRAVAPLVVSRIKVMSKLDIAERRLPQDGRISLRIAGRAVDVRVSTLPSGHGERVV
                     LRLLDKQAGRIELTSLGMDPRTQETMDEIIHKPHGIILVTGPTGSGKTTTLYAALERI
                     NDNTRNVMTVEDPIEYYIDGIGQTQVNTKVEMTFARGLRAILRQDPDVVMVGEIRDLE
                     TAQIAVQASLTGHLVLSTLHTNTAVGAVTRLRDMGIEPFLLSSSLIGVLAQRLVRVLN
                     PETREAFMAGEYERRLLNLESKGSPVELFRPSSEPGNGYRGRTGIYELIAVDDHMRTM
                     IHDGSSEQDLERYARTASPGIRDDGRRKVMAGETTLEEVLRVTRED"
     gene            6998..8215
                     /gene="gspF"
                     /locus_tag="VGE92_16580"
     CDS             6998..8215
                     /gene="gspF"
                     /locus_tag="VGE92_16580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HEY1315501.1"
                     /translation="MGAFEYTALDSQGKERKGLIEGDTPKHVRQLLRDKQLLPMEIQE
                     TAQRELKKNRARRFMRRGLSTLDLALLTRQLATLLHSGLPLEESLQAVAEQTEKPRVQ
                     RIILGVRGKVVEGHPLADGLREFPQAFPEIYRATVSAGEQSGKLDSVLERLSDYTETR
                     QVMGQQVSNALVYPIVLLVLSFVIVSFLLAYVVPQVVAVFESGHQELPIATRILIAMS
                     DLIRHYWFYALIVIGAATWGFMRWLKAPEARLRFDGFLLRVPLVGKLIRGLNTARFAR
                     TFSILTASAVPVLEALRISAEVVINLPMKRAVEDAALRVREGAPIGKSLAARKLFPPM
                     MIHLISSGESSGELEKMLERAAKNQEREMDGLLSTMTNLLGPFMVVFMGGVVLFIVIA
                     LLLPIFQLNELVK"
     gene            8276..8599
                     /locus_tag="VGE92_16585"
     CDS             8276..8599
                     /locus_tag="VGE92_16585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315502.1"
                     /translation="MSEKPESDEFEEEDVEDVAPGGDEVDVDHIMRDVESQRRRAPKG
                     AEPAWRRLERVMEERRTAELMSDFDDYEISDNDDVAESAAEDEDSDESQAHTAKTPRK
                     KKRSR"
     gene            complement(8612..>9841)
                     /locus_tag="VGE92_16590"
     CDS             complement(8612..>9841)
                     /locus_tag="VGE92_16590"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY1315503.1"
                     /translation="EPYYTKVEWQVGVSGLAHASPFDPPRSKPYPMPPMPVKSSGVLL
                     EKGARKLGLHPFPAPLAIASQPFRGRPACVHCGFCMGFGCEARAKSSSLYTMIPEAEA
                     TGNCEVRAASFVYHVAIDGSGRVTGVNYFDAEKRDLFQRARAVILSANGAETPRLLLN
                     STSSRFPQGLANSSGLVGKYLMFNQGASAHATFEHELNEYKSVQVTRLLHDFYDADPG
                     RGFYGGGGIDARINPQPAVWALSQGGDLPRWGAALKARIEAFPRSMVAAGHSTSLAVE
                     SNGISIDPTLKDAWGIPAIRVTYKDHPDDLACARFLQDRQVEIMHAAGARQVWRDDVA
                     EAESAVHLLGTARMGNDSATSVIDKYHRTHDVPNLFLCDGSSFVTSGRGQPTMTIQAL
                     AFRAADHIGRFAKRNEI"
BASE COUNT         1962 a         3063 c         2980 g         1836 t
ORIGIN      
        1 cggccgttat tgtcggtaag cgtgccgtgg tccatgacct gcagcagcaa attgaagaca
       61 tccggatggg ctttctcgat ctcatcgagc agcaatacgc aatgcggatg cttggcgatc
      121 gcttcggtca gtaagccgcc ctggtcgaat ccgacgtagc ccggcggtgc gccgatgagg
      181 cgcgagacgg tatgccgttc catgtactcg gacatgtcga aacgcacgaa ctcgaccccc
      241 atggcaatcg cgagctgccg ggtcacctcg gtcttgccga cacccgtcgg acccgagaac
      301 aggaacgagc ccacgggctt gcgttgatcc cccaatccgg agcgcgacat cttgatggct
      361 gacgacaagg cctcgattgc cttgtcctgg ccgaagatgg tcagcttcaa gttgcgctcc
      421 aggttcttca gcacatcgcg gtcggacaac gacacattct tcggcggaat ccgcgcaatc
      481 cgcgcaacca cgttctctat ttgcgtgacc gtgaccgaca gggcgcggtg ctcgaccggc
      541 tgtaaacgca agttggctcc cgcctcgtcg acgacgtcga tcgccttgtc cggcatgtgc
      601 cgatcgttga tgtgccgcgc ggccaattca gccgccgcgc gcagcgcatc gtcctcgtac
      661 ttgatgccgt gatgttcctc gtagcgcgat ttgaggccgc gaagaatgtc gacggtgtct
      721 tgcaccgagg gttcggtgat gtcgatcttc tggaagcgac gcgccaatgc atgatccttc
      781 tcgaaaattc cgcgatattc gttgtaagtg gtcgagccga tgcagcgcaa gtcgccgttg
      841 gccaaggccg gtttgatcag gttggaggcg tccatcacgc cgcccgatgc cgcgcccgcg
      901 ccgatgacgg tgtgaatttc gtcgatgaac agaatcgccc cgggctgctt tttgacctct
      961 gccaatacgg ccttcagccg cttctcgaaa tcgccccgat acttggtgcc ggcaaccagc
     1021 gagcccatgt cgagcgaata aatggtcgcg tccaatagca cgtcgggcac ctggttctcg
     1081 acaatcatgc gggcgaggcc ctcggcaatc gccgttttgc cgactccggc ttcgccgacg
     1141 tacaagggat tgttcttgcg ccggcggcac agtatttcga tggtgcgctc gacttcgagc
     1201 ttgcggccga tgagaggatc gatcttgcct ttacgcgccg actcgttgag gttggtcgcg
     1261 tatttctcca acgcgttgcc gccgccgtcg ttctcgcggt cggcgtccgg agaggcaccg
     1321 ccatcttcgc gctcgctgcg ctcctcgccg ccggccttgg acagcccgtg ggcgatgaaa
     1381 ttgacgacat cgaggcgcga cacatcctgc atgctgagca aatacgcggc gtgcgactgt
     1441 ttctcgctaa agattgccac cagcacgttc gccacggtga cttctttctt gccgctcgat
     1501 tgcacgtgaa acaccgcgcg ctgcagcacc cgctggaatc ccagggtagg ctgcacgtcg
     1561 cggtcctcgc cggccggcag gcgcggcgtg gtttgatcga tgaattgctt gagttcggtc
     1621 ttcagtttag cggtgtcggc gccgcaagac ttcaacactt cacgcacctt cggcgtatcc
     1681 acgatggcga gcagcagatg ctcgacggtc atgaattcat gccgtgcctc gcgggcgccg
     1741 gcaaacgcgt cattcaagca aaattccagt tcttgactta gcactgttac gcctcttcta
     1801 gcgttgccat cagcggatgc tgatgttgtc gcgcgtatgc gatcacttgg gccaccttgg
     1861 tctctgcaat ctcataagta aagaccccac agactccttt gcctttggta tgcacctcca
     1921 gcataacgcg agttgctttg gtacggtcca tcgtaaagaa acgctgcagg atgtcgacca
     1981 cgaactccat gggagtgtag tcgtcattga tcaacacgac ttggtagaga ggcggacgct
     2041 tgagcttcgg cgccgcttct tcgacgacca accccgtccc gtcccgccca cgttcgatgt
     2101 cactcatgaa taatcagttt atagggtgcg ttatgtcggt ttacaagcca tgtaagtcaa
     2161 accctccgtt tatgccctgt cgccaatcac ggtcacttta ctttaacaag taaaaacaaa
     2221 tgcttgagcg ttcaagcagg tagcccgtat gattgccgat cacggtaacc attcgcgacc
     2281 catacatgct cgatacgcgc tcaaatgaat ggctgtcccg tctgcagggt aacggccccc
     2341 ggctggtatc gctggcactc gccgcgctca tcgccgtgga actggcgcga atcgccattt
     2401 ccgtgctctc cggcggacca gtgaaatccc cgcaacccgt gctgacgagc gcggcgccac
     2461 gcggcctgca tgccggggtc gacgtccaag ccgtgacttc cgcccatctg ttcggcgtgg
     2521 ccgtcatcga tccggccgcc caggacccgg ccaatgcgcc gcagtcgacg gccaatttgg
     2581 tgctggcggg caccattgcg acccaggacc cgaagcacgg cgtggcgatc atcagcgatg
     2641 gcggtccctc aaaagtgtat tcggttggcg acaacatcgg cggcgcctct ttgcactcgg
     2701 tgtatctcga tcacgtaatt ctcgatcgcg tcggtgtact cgaaacccta ccgctgcccc
     2761 gcttgctcgg cccgggcatg cacgcagcgc cgatcgtgcg tcgcacgggc ggcgacccgc
     2821 gtaccgtggc ggcagtcgac aacatccgtc gcatggtgca gcaagacccc ggcattctcg
     2881 accaagtcat gcgcaccgtc ccctcgtacg ataacgcagc cggcaagctg cgcggctttc
     2941 gcgcttatcc cgggcgaaat cggctgattt tcaccaagct gggtctcaga tccggtgatt
     3001 tggtcacggc aatcaacggc acacagctcg atgatccgca gcgtagccaa gaggtattca
     3061 atacgatcca gacctcggac cgcgcaaccg tgaccattga gcgcaacggg cagaagcagg
     3121 acattacttt gaacattgca caggtcgcgg cgcaagcgac caaagatttg gaaggcgaca
     3181 acaccgggtc cggcgcgccg gcgaacggcg cgccgcagcc gccgccgagc ggcgctccca
     3241 acagtccgaa cgaaaatcca aacgaatgac gcgaacgaag gtcgaaacgc gttcaaccta
     3301 cgagtaccca tgatgccgaa ttccaacaac ccgcagacca agacccgaat cgccgctgtg
     3361 ttggcgcttg ctgctgcgtt attggtgtct gcggcgtcgc cggcggcgcc gcccgcgaag
     3421 ccgcagcagg acacgccgac gatcacgccc aactacaagg acgcggatct aagtcaaatc
     3481 atccaagcgg tggcggaagt caccggcaag aatttcatca tcgatccgcg cgtcaatgcc
     3541 aaggtgacca tgctatcggc gacgccgatg tcgccggccg cattctacga ggcgttcctg
     3601 tcggtattgc aggtgtatgg ctacgtcgca gtcccggccg gaaaagtcat caagatcgta
     3661 cccaatacgg atgcccggca gctgccggcc aacgatttgc cggcgagtgt gagctcgagc
     3721 tccgatgaga tcgtgacgca gatcattacc gtgaagaaca tcagcgcggc acaactggtg
     3781 ccgattctgc ggcccttcgt cccgcaatac ggccagctga ctgcctaccc ttcgggcaat
     3841 atgttgataa tctcggatcg cgccagcaat gtgagccgca tcgtgcgcat cgtcgagcgc
     3901 atggattcga gcgaagatga gccgatcgag gtaattgcgc tgcacaatgc cagcgcctca
     3961 gatgtggtgc gagtggtcaa ttcgatcaat cagggcggcc agggagccga gggcgccggg
     4021 tcagcggtaa agatcgtcgc cgacgaccgt accaatagta ttttgctcac cggcgagaag
     4081 tcgcagcgtc taaagctcaa agcgttgatc gtcaacatgg atacgccgct cgcgaccggc
     4141 ggcgacacgc aggttcggta tctgcgctat gccgacgccg aaaagattgc cgacaaactc
     4201 aagggccaag ccaccgccac ggccaaggcg caaggcgggc cgcccgcggc gggtgccgcc
     4261 ggcggcggcg gcggcgggac tgcgaatgtc gattccagcg tcaccatttg ggccgacgtg
     4321 gcgaccaacg ccctcatcat cacggcgccc cccaaaatca tgaagtcgct gatgaatgtc
     4381 atcgacaagc ttgatatccg ccgtgcccag gtgcaggtcg aggccatcat cgtcgaagtc
     4441 gacgtcaata agagctcgaa tatcggcgtg cagtggctcc tcgacggcgg caacaactac
     4501 ggctacggcg tgacgaatct accgggcagc ggcaccagca tcgtcgacct ggctgcggcc
     4561 gtattgggcg tgtcgcaagg tacggccacc tccgccgtca ccaccggcac ggccggcacc
     4621 accaccacgt cggggctcag cagttccacg gtgggcaccg gcgccacatt tgccatcggc
     4681 aaatacaatc agaataccgg caagggtttt gcggcgctga tccaggcgct acgcagcgac
     4741 ggcagcagca acatcatctc cacgccctcg ttgataacga tgaacaatga ggaagccgag
     4801 gtaaaagtca cgcaagagat tccgttgatc acgggttctt acagcagttc aaccgcgagt
     4861 gtgggcggca cgaccagtcc gttcacgacg attcagcgcg aagaggtggg cacaatcttg
     4921 aaagtcacgc cgcacatcaa cgagggaaat tccgtgcagc tcaagatcga gcaggaagac
     4981 tcctcacccg gggcgaagct cacggattcg gcggatatct cgaccaataa gcgctccatc
     5041 aagaccaccg tgctgatcga ggacggcggc atcatcgttc tgggcgggct gatgtcggac
     5101 accgtcacgg agagcgaaga ccgggtgccg ggactcggcg cgattccgct gctcggcaac
     5161 ctgttcaaat cgcgctcagg ttcgcggcag aagaaaaatc tcttggtctt cattcgtccg
     5221 aagatattgc gcgatgccga tgcgaccgag agcaccagcg agtcgaagta caacgaggtt
     5281 cgccaggatc aacgcacgct gaacaacgga cacataacgc tgctgcccgg acagaagcaa
     5341 ccggtggtgc cgcccatgcc tccgggatcg ggcttgccgg cgccgccgcc gtcgggcgcg
     5401 aacgcccagg gtgcaacgcc caacatgccg ccgccgtccg agccggcgcc ccagacgacg
     5461 cctgtgccga cacccgcgcc ggcaccgtcc aacccgccgc caaacccatg aacgcggaac
     5521 tcaatgatcc ggtcgagcaa cgcaaactgt cgtttccgtt tgcgaagcgt cacggcgtgc
     5581 tggtgcgcgg catcgccgag ggcgtggccg ataccgtgtg ccgtccgggc gtgacgccgc
     5641 agtccctggc tgaggttcgg cgtttcgtgg gcgtaccgtt gaagctgcag cgcgtatcga
     5701 gcgaggcctt cgactccttg ttgcgcgtgg cctatgaggc cggctcgggc gccgcaatgc
     5761 agatgctcga gggcttggac gacaataccg accttgcgca tctggccgag gacattcccg
     5821 agtcctccga cctgctggaa agcgatgacg aagcgccgat catccgcctg atcaatgcgt
     5881 tgttgacgca ggcggtcaag gaaaacgcct cggatattca tattgagccc tacgagaacc
     5941 gcctggtgat ccgctttcga gtcgacggcg tgttgcgcga agttctgcaa accaagcgcg
     6001 ccgtcgcgcc gctggtggtg tcgcgtatca aggttatgtc aaagctcgat attgccgagc
     6061 ggcggctgcc gcaggacggc agaatttcgc tgcgcatcgc cggccgtgcg gtcgacgtgc
     6121 gcgtctcgac gctgccttcc ggacatggcg aacgcgtggt gctgcgactg ctggacaagc
     6181 aggccgggcg catcgaactt acctcgctcg gcatggatcc gcgtacccag gaaacgatgg
     6241 atgaaatcat ccacaagccg cacggcatca ttctggtgac cggacctacc ggctcgggca
     6301 agaccaccac gctgtacgcg gcactcgaac gtatcaacga caacacccgc aatgtcatga
     6361 ccgtcgagga tccgatcgag tactacatcg acggcatcgg ccagacgcag gtaaacacca
     6421 aggtcgagat gaccttcgcg cgcggcttgc gggcgattct ccgccaagat cccgacgtgg
     6481 tgatggtcgg tgaaattcgc gacctcgaaa ctgcgcagat cgccgtgcag gcgagcttga
     6541 ccggtcacct ggtgttgtcg acgctgcaca ccaataccgc ggtcggtgcg gtcacacgct
     6601 tgcgcgacat gggtatcgag ccgttcctgc tatcgtcgag tttgatcggc gtattggcgc
     6661 aacgcctggt gcgcgtgctc aacccggaaa cgcgcgaggc tttcatggcc ggcgaatatg
     6721 aacgccgcct gttgaatctc gagtccaaag gcagtcccgt agagttattc cgcccgagca
     6781 gcgagcccgg aaacggctac cgcggacgaa ccggaatata cgaactcatt gccgtcgatg
     6841 atcacatgcg gaccatgatc catgacggct cctcggaaca ggatctggag cgctatgcgc
     6901 gcaccgcaag ccccggaatt cgcgatgacg gccgcagaaa ggtgatggcg ggcgagacga
     6961 cgctcgaaga agtgctgcgc gtcacccgcg aagactgatg ggtgcgttcg agtacacggc
     7021 cctcgattct cagggaaaag agcgcaaggg tctgatcgaa ggcgatacgc cgaagcacgt
     7081 acggcagctg ctgcgcgaca agcaactgct gccgatggag attcaggaaa ctgcgcagcg
     7141 cgaactgaag aaaaatcgcg cccgacggtt catgcgccgc ggtttgtcga ccctcgactt
     7201 ggccctgctg acccgccaac tcgccacttt gctgcactcc ggcctgccgc tcgaagaatc
     7261 gctgcaggcc gtcgccgaac agaccgaaaa acctcgagtg cagcgcatta tattgggggt
     7321 gcgcggcaag gtggtcgagg ggcacccgct ggccgacggg ctgcgggaat ttccgcaggc
     7381 atttccggaa atctatcgtg caaccgtgtc cgccggcgag caatcgggca agcttgattc
     7441 cgtactcgaa cgcttgtcgg attacaccga aacccgccag gtgatgggcc agcaggtgag
     7501 taacgccctg gtctacccga tcgtcctgct ggtcctgtct ttcgtcatcg tgtctttctt
     7561 gctcgcttac gtggtgccgc aggtcgtggc cgtattcgag tccggacacc aagaattgcc
     7621 gatcgcgaca cggattctca tcgccatgag cgatcttatc cgtcactatt ggttttacgc
     7681 gttgatcgtg atcggcgccg ccacctgggg attcatgcgc tggctcaaag cccctgaggc
     7741 gcgcctgcgg ttcgatggct tcctgctacg ggtgccgttg gtgggcaagt tgattcgtgg
     7801 tctgaatacg gcgcgattcg cccgaacttt cagtattctg acggcgagcg cggtaccggt
     7861 gctcgaggcg ctgcgaatat cggccgaggt ggtcatcaat ctgcccatga agagggccgt
     7921 cgaggatgcg gctctgcgcg tgcgcgaggg cgcgcccatc ggcaagtcgc tggctgcgcg
     7981 caagctgttc cctcccatga tgatccacct gatcagcagc ggcgaaagca gcggcgagct
     8041 cgagaaaatg ctcgagcgcg cggcaaagaa tcaggaacgc gagatggacg gcttgctctc
     8101 gaccatgacc aatctgttgg gcccgttcat ggtcgtgttc atgggcggag tcgtcctctt
     8161 catcgtgatc gccctcctct tgcctatatt tcaactcaac gagctcgtta agtagcggac
     8221 tgtgcgctag ggtcttgcac attgggcgcc tttccgttta taacgcgccc gtgtcatgag
     8281 cgaaaagcct gaatctgacg aattcgagga agaggacgtc gaggatgtgg cccccggcgg
     8341 cgatgaggtt gacgtcgatc acatcatgcg cgacgtcgag tcgcagcgtc ggcgcgcgcc
     8401 aaagggtgcg gagcctgcgt ggcgccgcct ggagcgcgtc atggaagagc ggcgcaccgc
     8461 cgaactcatg agcgacttcg acgactacga aatctcagac aacgatgacg tggcggaatc
     8521 ggcggccgag gacgaggact cggacgaatc tcaggcgcac accgcgaaaa ctccgcgcaa
     8581 gaaaaaaaga tcgcgctgat cgggctggtg ctcaaatttc gttgcgcttg gcgaatcggc
     8641 cgatgtggtc cgccgcgcgg aacgccagcg cctggatcgt catggtcggt tgaccgcggc
     8701 ccgatgtcac gaaactcgac ccgtcacaca ggaatagatt cggcacatca tgcgtgcggt
     8761 gatatttatc gatcaccgag gtggcggaat cattgcccat gcgcgcggtg ccgagcaggt
     8821 gcaccgccga ctcggcttca gccacatcgt cgcgccacac ctgtcgtgca cctgccgcgt
     8881 gcatgatctc gacttggcgg tcctgcaaaa atcgcgcgca ggccagatcg tcggggtgat
     8941 ccttgtaagt gacccggatg gcggggatac cccaggcgtc cttgagcgtc ggatcgatgc
     9001 tgatgccgtt cgattcgacg gcgagcgaag tcgagtgacc cgccgccacc atggagcgcg
     9061 ggaaggcctc gatgcgcgcc ttgagagccg caccccaacg cggcagatca ccgccctgag
     9121 ataacgccca caccgccggc tgcggattga tgcgcgcgtc gatcccgccc ccgccgtaga
     9181 acccgcgccc cgggtcggcg tcgtagaagt cgtgcagcag gcgcgtcacc tgcacgctct
     9241 tgtactcgtt gagctcgtgt tcgaacgtcg catgcgccga ggcgccttgg ttgaacatga
     9301 ggtacttgcc gaccagaccg ctcgagttgg caaggccctg cggaaaacgg ctgctggtgg
     9361 aattgagcag caagcgcggc gtctccgcgc cgttcgccga caggatcacc gccctcgctc
     9421 tctgaaaaag atcgcgtttc tcggcatcga aataattcac gccggtgacg cggccgctgc
     9481 cgtcgatcgc cacgtggtag acgaagcttg ccgctcgaac ctcgcaatta cccgtggcct
     9541 cggcttccgg aatcatcgtg tacagcgacg aggacttcgc ccgcgcttcg cagccgaatc
     9601 ccatgcagaa tccgcagtgc acacacgcag ggcggccgcg aaatggctgc gacgcgatgg
     9661 cgagcggcgc cggaaaggga tgcaggccga gcttgcgcgc gcccttctcc aacagcacgc
     9721 cggaagattt caccggcatc ggcggcatcg ggtacggttt gctgcgcggc gggtcgaacg
     9781 gactggcatg ggcgagtccc gagacgccta cctgccactc gaccttggtg taatacggct
     9841 c
//
LOCUS       DASWZT010000580         9325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_72565, whole genome shotgun sequence.
ACCESSION   DASWZT010000580 DASWZT010000000
VERSION     DASWZT010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9325
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72565"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1319
                     /locus_tag="VGE92_16595"
     CDS             <1..1319
                     /locus_tag="VGE92_16595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1315504.1"
                     /translation="RVSSRPGKGSTFYFSVTLATEHAEVRERSAAALNSTRMLLVDDN
                     ATARGVLRRHLMSWGVMPSEAGSAHEALDILRRSLGAEFDAVIVDGQLPDMGGAQLVA
                     SIRDLPELTRVPIIVMNSSIAAPPTQSDRRDTATTWLNKPVRRTQLHDCLAALLTGQH
                     DVGARPQAAIQNPTAVEAGGQRRSRVQRVLLVEDNPVNQEVAFAMLQELGVSAVRAWN
                     GEEALEKLRADRYEAVLMDCQMPKLDGYAATSRFREWEQEHQRSRTPVVALTANALTG
                     DAEKCFAAGMDYYLTKPFTIDQLYEVLESCVSDEAAAAGAAPRHDNAILDQRTLGSIK
                     AMHKTGSPDLLARVVRLYTSSSRDLIEALRAAAAAGDTTAMMHAAHALKSSSAHVGAV
                     VLAELCKEVELAAGGGKLDFASMLVERVLEEHEQVLQALLDQGIAA"
     gene            complement(1324..1611)
                     /locus_tag="VGE92_16600"
     CDS             complement(1324..1611)
                     /locus_tag="VGE92_16600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315505.1"
                     /translation="MALSAFDRGKLKIAAHAREELERAGICFESIQCKSGGTETHPDI
                     ARLTVTVNGAAAHVDLSAYEVEECELLVFGDAWYKIAAFIERLRERPTPAG"
     gene            1689..2264
                     /locus_tag="VGE92_16605"
     CDS             1689..2264
                     /locus_tag="VGE92_16605"
                     /inference="COORDINATES: protein motif:HMM:NF018302.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EipA family protein"
                     /protein_id="HEY1315506.1"
                     /translation="MNSRSLRLSALLAGLLWFAVAIPAADTPATHDTYSDDEIAKAAS
                     DFFSSGAKELSEVLAKVLKEKGHPVAIIRGEEAGGAIGVGLRYGRGELVYKGSPAHKV
                     YWQGPSIGFDVGANAVKTFILVYDLPNTAALYKRFPGVEGSLYFVGGFGVNYVQRDGI
                     ALAPVRFGVGWRQGIALSYMNFSREKRYNPL"
     gene            2371..3408
                     /gene="bchI"
                     /locus_tag="VGE92_16610"
     CDS             2371..3408
                     /gene="bchI"
                     /locus_tag="VGE92_16610"
                     /EC_number="6.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0010007 - magnesium chelatase complex
                     [Evidence IEA];
                     GO_function: GO:0016851 - magnesium chelatase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase ATPase subunit I"
                     /protein_id="HEY1315507.1"
                     /translation="MQSARRPVFPFSAIVAQDEMKLALISSAVDPAIGGVLVFGDRGT
                     GKSTAVRALAALLPPMSAVVGCRYSCDPADADSLCVECCERLRHGALKKHLIPVPVVD
                     MPLGVTEDRVVGALDLERALSQGVKAFEPGLLARANRGFLYIDEVNLLEDHLVDLLID
                     VAASGENLVEREGLSVRHPSRFVLIGSGNPEEGELRPQLLDRFGLSVEVTTPAELELR
                     VAVVRRRDEFERDRSGFIAQWKKQDDDLRRRIVAARKRLEKIDVSDAVLESAARLCML
                     LGTDGLRAELTLIRAARAFASFENARAVQVSHLKRVAPAALRHRLRRDPLDESGSAAR
                     IERAVQETLGA"
     gene            3405..5150
                     /locus_tag="VGE92_16615"
     CDS             3405..5150
                     /locus_tag="VGE92_16615"
                     /EC_number="6.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF009943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase subunit D"
                     /protein_id="HEY1315508.1"
                     /translation="MNAQNDASWVASLFAVDPVATAGVCLRSQVQPARDQWLQLLREL
                     LSPGGVRRIPFNISDARLLGGLDLAATLKANRPIAECGVLAASDGGVVIITMAERLTR
                     HTAACIGAVLDAGEAVSAREGMWSHPARVGVVALDEGLAEDEVVPHCLLDRLAFSLDF
                     NGFSVRSPIVASHEPADILAARALLPKVRGAQGIIESLCATAQALGVESARAAVYAYR
                     VARIAAALHGRTDIDEQDAVLAGRLVLAARATRLPVTPQTASDPPTPDSIEPGVGDAT
                     PPAESSEATRVGELQTQVLAATQAAIPAGLLMQLERNAILPRHRPSGSGRAGELRNAG
                     ARGRPAGVRSAVPQGGARLNVIETLRAAAPWQALRGRPAQAGSRLRIQASDCRVTRYQ
                     QRARTLTVFVVDASGSSALHRLAEAKGAVELLLADCYIRRDQVAVIAFRGRAAELLLP
                     PTRSLPRAKRSLAGLPGGGATPLAAAIDAAVTLASQARRRGETPTLVMLTDGRANIAR
                     DGAAGREAAHADALKAASGVRLAKTAAILIDTAPRPNTLARDVANAMGAQYVALPFAN
                     SRALSDIVRAAAVRT"
     gene            5152..6021
                     /gene="bchO"
                     /locus_tag="VGE92_16620"
     CDS             5152..6021
                     /gene="bchO"
                     /locus_tag="VGE92_16620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase BchO"
                     /protein_id="HEY1315509.1"
                     /translation="MWEREKSTWPNHELSRFVEAAGSRWHVQQMGSGAPLLLVHGTGA
                     STHSWRDILPLLGKHYSVLAADLPGHGFTESAGTTRSSIEGMSAALSMLLGALRFNPL
                     YCVGHSAGAVILCRMALDRRIDPRVIISLNGAFVPLGGPAGVLFSPLAKLLARSSLLP
                     RLLARRAGNPASVARVLDGTGSKVDARGLELYTRLVNNPKHLQGALGMMGNWDLRTFA
                     RDLPRLTTPLALVAAENDRTVAPHQAFEIQQRLANASVCSLSGVGHLAHEEQPVLVSE
                     KILSICRAHAGTP"
     gene            6018..7514
                     /locus_tag="VGE92_16625"
     CDS             6018..7514
                     /locus_tag="VGE92_16625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase"
                     /protein_id="HEY1315510.1"
                     /translation="MNGRFPHAIVIGSGFGGLAAAIRLGARGYRVTVLEQLDAPGGRA
                     YVFRQDGFTFDAGPTIITAPFLLEELWRLCNRRFADDVELRALQPFYRIRFHDGGVLD
                     CSDDDTRMREQIERIAPGEFVGYQRFLKASEDIYDVAFTRLAHLPFDSFQDMLRLAPD
                     LLRLHSLRSIHALAASFVKSPKLRFALSFHPLFIGGNPFSTTAIYGLIAFLERRWGVH
                     FAVGGTGSIVAGMVKLIEGQGNQVRCGVRVASIAVHERRAVGVVCEDGHPLAADIVVS
                     NADSASTYRDLIPRAARRRWSDAKLDRARYSMSLFVWYFGTSRQYPDVPHHTILVGPR
                     YRELLRDIFQRKVLAEDFSLYLHRPTASDASLAPAGCDAFYVLSPVPNLQSKTDWRVV
                     GESYRRAIAECLSGCVLPDFERHVISSRIMTPLDFRDRLSSYRGAAFGLAPVLSQSAW
                     FRPHNRSEEVENLFLVGAGTHPGAGVPGVLSSARVLDTVVPDAKVFLR"
     gene            7492..8574
                     /locus_tag="VGE92_16630"
     CDS             7492..8574
                     /locus_tag="VGE92_16630"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEY1315511.1"
                     /translation="MPKSSSASGFASAADVAACRALLRGGSRTFFAASLVLPLGVRKP
                     ATGLYAFCRMADDAIDQQVDRAAALRELHARLEAIYAGTPSDIAADRAFGDCVSRHAI
                     PQALPLALLEGFAWDAEGRRYQTLSNLMCYAVRVAGSVGVMMAMAMGVRDSIALARAC
                     DLGVAMQLTNIARDVGEDARCGRLYLPLDWLLEAGIEPQAWLAKPRFSSALGTVIERL
                     LNSAEQLYARSEAGLARLPAACRPGMNAARLLYAEIGHEVARRKYDSVSQRAVVPWQR
                     KARILADALFAATARPSAADAGALHEAQFLLAPLPTAVLEPPLPIESARERTRWPRIE
                     DRVIWLVDLFERLERRERLPVGGIRT"
     gene            complement(8564..>9325)
                     /locus_tag="VGE92_16635"
     CDS             complement(8564..>9325)
                     /locus_tag="VGE92_16635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315512.1"
                     /translation="VALYGKRANRWTMTERGAAQLRRETSRLSIGPSEMRWSGDALEI
                     SVNEVCAPLPRRVRGTIRLFPIALCENGFSLDGHGRHRWTPFAARARIEVALSQPSVT
                     WAGDGYFDSNRGTEPLEDAFTDWTWSRASLPQRTIVLYDVNARAAAAESPGAGLLALK
                     FAANGEVERIEPPPPAALPRTGWRVARHTRADAGHAVRVIKTLEDAPFYSRTLLDTRL
                     LGLRSPAIHESLDLNRFRAGWVQCLLPFRMPRITF"
BASE COUNT         1528 a         2948 c         3125 g         1724 t
ORIGIN      
        1 tccgcgtgtc gagcaggccg ggcaagggat cgaccttcta tttttccgtg actctggcaa
       61 ccgagcacgc cgaggtgcgc gaaagaagtg cggccgcgct gaattccacc cgcatgctgc
      121 tggtggacga caatgcaacg gcgcgcggcg tgctgcggcg ccatctcatg agctgggggg
      181 tcatgccaag cgaggcagga tcggcgcacg aggcgctgga cattctgcgc aggtcgctcg
      241 gcgcggaatt cgacgcggtg atcgtcgacg gtcagctgcc cgacatgggc ggggcgcaac
      301 tcgttgcatc gattcgcgat cttccggaat tgacgcgtgt tccgatcatc gtcatgaact
      361 ccagtatcgc ggcgccgccc acgcagagtg accggcgcga caccgcgacg acgtggttga
      421 ataagccggt gcgccggacg caactgcatg attgcctcgc cgcgctgctc accggccaac
      481 acgatgtcgg cgctcggccg caggccgcaa tacaaaaccc gactgcggtc gaggcgggcg
      541 ggcagagacg atcgcgtgtg cagcgggttc tgctcgtcga agacaatccg gtcaatcagg
      601 aagtggcctt cgccatgctg caagagttgg gcgttagcgc ggtgcgcgcc tggaatggtg
      661 aggaggcgct cgagaagctg cgcgccgatc gttatgaagc ggtcctcatg gattgccaaa
      721 tgccgaagct cgatggctac gcggcaacca gccgctttcg cgagtgggag caggagcacc
      781 agcgcagccg caccccggtg gttgcactga ctgccaacgc cctcacggga gacgccgaga
      841 aatgctttgc ggccggcatg gattactact tgaccaaacc attcacgata gatcagctgt
      901 acgaagttct cgagtcgtgc gtctccgatg aggcggccgc cgccggtgca gcaccacggc
      961 acgacaacgc cattctcgac cagcgcacgt tgggttcgat caaggcaatg cacaaaacgg
     1021 gcagcccgga tctcttggca agggtcgtgc gtttgtatac atcgagctct cgcgatttga
     1081 tcgaggcgct gcgcgccgcg gcggcggccg gcgatacgac ggcaatgatg cacgcggcgc
     1141 acgcgctcaa atcctcgagt gcgcatgtgg gcgcggtggt gctggcagaa ctatgcaaag
     1201 aagtcgagct ggccgccggc ggcggtaagc ttgacttcgc cagtatgctg gtggagcggg
     1261 tcctggaaga gcacgagcag gtactgcagg ccctgctcga tcaggggatc gcagcctgat
     1321 ttgtcagccc gcaggcgttg gccgctcgcg cagccgctcg atgaaagcgg cgattttgta
     1381 ccaggcatcg ccgaacacca acaattcgca ctcttccacc tcgtacgccg acagatcgac
     1441 gtgcgcggcc gcaccgttca ccgtcacggt gagtcttgcg atatccggat gcgtttcggt
     1501 gccgcccgat ttgcattgaa tcgattcgaa gcagataccg gcccgctcga gctcttcgcg
     1561 tgcatgagcg gcgattttca gcttgcctcg atcgaatgcg ctgagggcca tcgttttacg
     1621 gatattactg gagcgccgcc gtgcgcgaaa tacttgcgta atggcaaacc cgcaaccaga
     1681 atgtggccat gaattccagg tcattgcgcc tctcggccct gttggcgggc ctcctctggt
     1741 tcgcggttgc catccccgcg gccgataccc cggcgactca cgacacctac agcgacgacg
     1801 aaatcgccaa ggcggcgtcg gattttttta gcagcggcgc caaggaattg tcggaagtgt
     1861 tggcgaaagt gctcaaggag aagggtcacc cggtggcgat catccgtggc gaagaagcgg
     1921 gcggcgccat cggtgtcggc ctgcgctatg gccggggcga gctggtttac aaggggagtc
     1981 ccgcgcacaa agtctactgg cagggaccgt ccatcggctt cgacgtcggc gccaacgccg
     2041 tcaagacctt cattctggtg tacgacctgc cgaatactgc cgcgctttat aagcgctttc
     2101 cgggggtcga aggcagcttg tactttgtcg gcggcttcgg cgtgaactat gtgcaacggg
     2161 acggcatcgc cttggcgccg gtgcgcttcg gggtgggttg gcgccagggc attgcgctca
     2221 gctacatgaa cttctcccgc gagaagcgct acaacccgtt gtagcagggg attgcggccg
     2281 gcgagaagcg cggcagtacc gagcaaaatg ctagctgcgg cggctggcgc ttggcgcact
     2341 gttgcggttt gtgccaatgt gcgcacacct atgcaatcag ctcgccgtcc ggtatttcca
     2401 ttttccgcaa tcgtcgcgca ggacgagatg aaacttgcgc tgatcagttc ggcagtcgat
     2461 ccggcgatcg gcggcgtctt ggtgttcgga gatcgaggca ccggcaagtc aacggcggtg
     2521 cgggccttgg cggcgctgct gccaccgatg tctgcggtcg tcggctgccg ctactcgtgt
     2581 gatccggcgg acgccgactc cctgtgcgtc gagtgctgtg aacgcttgcg tcacggcgca
     2641 ttgaaaaagc acctgatacc ggtgccggtg gtcgatatgc cgttgggcgt caccgaggat
     2701 cgggtggttg gtgcgctcga tctcgagcgg gcactgtcac agggcgtgaa ggctttcgag
     2761 cccggcctgc tggcgcgagc gaatcgcgga ttcctgtata tcgacgaggt gaatctgctg
     2821 gaagatcatt tggtggacct cctgatagac gtcgcggcat cgggcgagaa cctggtggag
     2881 cgcgagggac tgagtgtccg ccacccatcg cgattcgtac tgatcggcag cggaaatccc
     2941 gaggaggggg aactgcggcc gcagctgctg gaccggttcg ggctatcggt tgaggtgacg
     3001 acgcccgccg aactcgaatt gcgcgtcgcg gtggtcaggc ggcgcgacga gttcgaacgc
     3061 gatcgcagcg gcttcatcgc ccaatggaag aagcaagatg acgacctgcg ccggcgtatc
     3121 gttgcggctc gcaaacgact cgaaaaaatc gacgtgtcgg acgccgtgtt ggagagcgcc
     3181 gcgcgcctct gcatgctgtt gggcaccgat ggtttgcgcg cggaactcac gctgatccgc
     3241 gccgcacgcg cgttcgcaag cttcgagaac gcgcgcgcgg tgcaggtgtc gcacctcaag
     3301 cgcgtggctc ctgcggcgct gcgtcaccgc ctgcgacgcg accctctcga tgaatccggc
     3361 tcggctgcgc ggatcgaacg agctgttcag gaaacgctgg gcgcatgaac gcgcagaacg
     3421 atgcgtcttg ggtcgcctcg ctgttcgccg tcgacccggt ggcgacggcg ggggtctgtc
     3481 tgcgctcgca ggttcaaccg gcgcgcgatc aatggttgca gctgctgcgc gagttgctgt
     3541 cgccgggcgg cgtgcgccgg attcctttta acatcagcga cgcccgcttg ctcggcggat
     3601 tggatttggc cgcgaccctc aaggcgaatc ggccgatcgc ggagtgcggt gtactggcgg
     3661 ccagcgacgg cggcgtcgtg atcattacca tggccgaacg gctgacgcga cacacggctg
     3721 cttgcattgg cgccgtgctc gacgccggcg aagccgtgtc ggcgcgcgaa gggatgtgga
     3781 gccatcccgc gcgcgttgga gtcgtggcac tcgatgaggg cctggccgag gacgaagtcg
     3841 tgccgcattg cttgctcgat cgtctggcgt tttcgctgga tttcaatggc ttcagcgtgc
     3901 gctcgcccat cgttgcgtcg catgagcctg cagatattct cgcggcgcgt gcgttgctgc
     3961 cgaaagtccg aggagcacag ggaatcatcg agtcgctgtg cgcaacggcg caggccctcg
     4021 gcgtcgaatc ggcgcgggct gctgtctacg cgtatcgagt ggcacgcatc gcagccgcgt
     4081 tgcacggccg aaccgatatc gacgaacagg acgcggtcct ggctggaaga ttggtgctcg
     4141 cggcgcgtgc gacgcgcttg ccggttacgc cgcaaacagc gtccgacccg ccgacgcccg
     4201 acagcatcga gcccggtgtg ggcgatgcga cgccgcccgc agagtcgtcc gaagcgacgc
     4261 gtgtgggtga gttgcagact caagtgctcg cggcgacgca ggccgcaatt ccggccggat
     4321 tgctgatgca actggagcgc aacgcgatac ttcctcgtca ccggccgagc ggcagcggac
     4381 gcgccgggga gttgcgcaac gcaggcgcaa ggggacgtcc ggccggcgtg cgcagcgctg
     4441 tgccgcaagg cggcgcacgc ctcaatgtga ttgaaacgct gcgcgcggcg gcgccctggc
     4501 aggcattgcg cggccgcccg gcgcaggctg ggagccggtt gcggattcag gcatccgatt
     4561 gccgggtcac ccgctaccag caacgagctc gcaccttgac ggtatttgtc gtcgatgcgt
     4621 cgggttcgtc cgcactgcat cgcttggccg aggcaaaagg agcggtcgag ttgttgttgg
     4681 cggattgcta tatcaggcgc gatcaagtcg cggtcatcgc tttccgcggc cgcgctgccg
     4741 agctgctatt gccgccgacc cgatcgctgc cacgcgccaa gcgcagcctt gccggcctac
     4801 cgggcggcgg cgccacgccg ctggcggccg ccatcgacgc agcagtcact ttggcgagcc
     4861 aggcgcggcg ccgcggcgaa acaccgacgc tcgtcatgct gaccgacggg cgcgccaaca
     4921 ttgcgcgcga tggcgccgca gggcgcgaag cggcgcatgc cgatgcgctc aaggccgcgt
     4981 caggcgtgcg gctggcgaag acggccgcca tattgatcga tacggcgccg cgcccgaaca
     5041 cgctcgcccg cgacgtcgcg aacgcaatgg gcgcgcagta cgtcgccttg ccgttcgcca
     5101 attcgcgtgc cctgtccgat atagtccggg ccgccgcagt gaggacttga attgtgggag
     5161 cgtgagaagt cgacgtggcc gaaccacgaa ttgagccgct tcgtcgaggc cgcgggcagc
     5221 cgctggcacg tgcaacagat gggcagcggg gcgccgctat tgctggtgca cggcaccggc
     5281 gcctccacgc actcctggcg cgacatcctg ccgctgctcg gcaaacacta ctccgtgttg
     5341 gcggcggacc tgccgggaca tggtttcacg gagtccgcgg gcaccacacg cagttccatc
     5401 gagggcatga gcgcagcgct ttcgatgctg ctcggggcgt tgcgattcaa tccactctac
     5461 tgtgtcgggc actccgccgg cgcggtcatt ctgtgtcgca tggcgctcga tcgacgcatc
     5521 gacccacgcg tgatcatcag cctcaatggt gcgttcgtac cgttgggcgg cccggccgga
     5581 gtgctgtttt cgccgctggc caagctgctc gcgaggagct cgttgctgcc gcggctattg
     5641 gctcgccgcg caggcaatcc ggcgagtgtg gcccgggttc tcgatggcac gggatcgaaa
     5701 gtcgatgcac gtggcctcga gctgtatacg cgcctcgtca acaaccccaa gcatctccaa
     5761 ggtgcgctcg gcatgatggg caattgggat ctgcgcacct ttgcaagaga tttgccgcgg
     5821 ttgacgacac cactcgccct cgtggcggcg gaaaacgatc ggaccgttgc gccgcatcag
     5881 gcgttcgaaa tccagcagcg cctcgcgaac gccagtgtct gcagcttgtc cggtgtcggc
     5941 cacctggcgc acgaagagca gccggttttg gtctctgaaa agatcttgag catctgccgc
     6001 gcgcacgcgg ggaccccgtg aacggccgct ttccgcatgc catcgtcatt ggcagcggct
     6061 ttggcggact cgcggccgcc atccgcctgg gcgcacgcgg ctatcgcgta accgtgctcg
     6121 agcagctgga tgcgccgggc gggcgggcct atgtcttccg ccaggacggc ttcaccttcg
     6181 acgccggccc gacgatcatc acggcgccgt ttctgctcga ggagctatgg cgcttgtgca
     6241 accgccgctt cgccgacgat gtcgaactgc gcgccttgca gcctttctac cgcatccgct
     6301 ttcacgacgg cggcgtactc gactgttcgg acgacgatac ccgcatgcgc gagcagatcg
     6361 agcggattgc gcccggtgaa ttcgtcggct atcagcgctt tctcaaggcc agcgaagata
     6421 tctatgacgt ggcttttaca cggctcgcgc acctgccttt cgattccttc caggacatgc
     6481 tgcgcctggc gccggacctg ttgcggctgc acagcttgcg cagcattcac gccctggccg
     6541 cttccttcgt caagagcccg aagctgcggt tcgcgttgag tttccatccg ctgttcatcg
     6601 gcggtaatcc gttcagcacc accgcgatct acggattgat cgccttcctc gagcgccgct
     6661 ggggcgtgca tttcgccgtc ggcggaaccg gcagcatcgt cgccggcatg gtgaagctga
     6721 tcgagggaca gggaaaccaa gtacgctgtg gagtgcgggt tgcgtcgatt gcggtccatg
     6781 agcggcgcgc tgtcggggtg gtgtgcgagg acggtcatcc gcttgccgcc gacatcgtcg
     6841 tatcgaacgc cgactcagcc agcacctatc gggatctcat tccgcgggcg gcgcggcgcc
     6901 gctggagcga tgccaagctc gaccgggcac gttactcgat gagcctgttc gtctggtatt
     6961 tcggcacgtc caggcagtat ccggatgtgc cgcaccatac gatcctggtc gggccgcgct
     7021 accgcgaatt gctgcgcgac attttccagc gcaaggtact ggccgaggat ttcagcctgt
     7081 acctgcaccg gccgacggct tcggatgcct cgctcgcgcc cgcgggctgc gacgcattct
     7141 atgtactgtc gccggttccg aatctgcaaa gcaagaccga ttggcgggtt gtcggggaga
     7201 gctatcggcg cgccatcgca gagtgcttga gcggctgcgt cctgccggat ttcgagcggc
     7261 atgtgatctc gtcgagaatc atgacgccgc tcgattttcg cgaccggctg tcctcttatc
     7321 gaggcgctgc cttcggcttg gcgcccgtcc tgtcgcaaag cgcctggttc cgtccgcaca
     7381 atcgcagcga agaggtcgag aatctgttct tggtcggtgc cggtacgcat cccggcgccg
     7441 gcgtgcccgg ggtgctttct tccgcgcggg ttcttgatac agtagtgccc gatgccaaag
     7501 tcttcctccg ctagcggatt tgcctccgct gccgatgtgg ccgcgtgccg cgcgctgctg
     7561 cgcggcggtt cgcggacgtt tttcgccgct tcgctggtgt tgccgctcgg cgtgcgcaag
     7621 ccagcgaccg gcctgtacgc gttttgccgg atggccgatg atgcgatcga tcagcaagtc
     7681 gatcgcgcgg cggcgctgcg cgagctgcac gcccgcctcg aggcgatcta cgccggcaca
     7741 ccgagcgaca tcgccgccga ccgagcgttc ggcgactgcg tatcgcgcca cgcgattccg
     7801 caggcactgc cgctcgcgct gctcgaggga tttgcctggg atgccgaagg acgccgctac
     7861 caaactttgt cgaacctgat gtgctacgcg gtgcgcgtgg caggtagcgt gggggtcatg
     7921 atggcgatgg cgatgggtgt tcgcgactcg atcgcgctcg ctcgagcctg tgatttaggc
     7981 gtcgcgatgc aattgaccaa catcgcgcgc gacgtgggcg aagacgcgcg ctgcgggagg
     8041 ctttatctgc ccctcgactg gctgctcgag gcgggtatcg agccgcaggc ctggttggcg
     8101 aaaccccgct tcagcagcgc gctcggcacg gtcatcgagc gccttttgaa cagcgcggag
     8161 cagctttacg cgcgcagcga agccggtctt gcacgactgc cggcagcctg ccgcccggga
     8221 atgaacgccg cgcgtcttct gtacgcagag atcggtcacg aggtcgcgcg ccgcaaatac
     8281 gattcggtca gtcagcgtgc cgtcgtcccc tggcagcgca aggcgcgaat tctggcggac
     8341 gccttgttcg ccgcaaccgc gcggcctagc gccgcagacg ccggcgcgtt gcacgaggcc
     8401 cagttcctgc tcgcgccctt gccgactgcg gttctcgagc ctccgttgcc gatcgagtcg
     8461 gcgcgcgagc ggacgcgctg gccgagaatc gaagaccgcg tcatttggct ggtggattta
     8521 ttcgaacggc tcgaacgccg cgagcgattg ccggttggcg ggatcagaac gtgatccgcg
     8581 gcatgcgaaa cggcagtaag cattgtaccc agccggcgcg aaagcggttc agatccaggc
     8641 tctcgtggat ggccggtgag cgtaggccca gcaagcgcgt gtccaacagc gtgcggctgt
     8701 agaagggtgc gtcctccaac gtcttgatca ctcgcaccgc gtgcccggca tcggcacggg
     8761 tgtggcgcgc gactcgccag ccggttcgcg gcagcgcggc cggcggcggc ggttcgatcc
     8821 tctcgacttc tccgttggct gcaaatttta gggcgagcaa gccggctccc ggcgattcgg
     8881 cggccgcggc tctcgcgttc acatcgtaga ggacgatcgt gcgttgcggc agactcgccc
     8941 gcgaccaggt ccagtccgta aatgcatcct ccaggggctc ggtgccgcgg ttggaatcga
     9001 aataaccgtc tcccgcccac gtaaccgaag gctgcgacag ggccacttcg attcgcgccc
     9061 gcgccgcaaa cggcgtccac cgatgtcgcc cgtgcccgtc cagagaaaac ccgttttcgc
     9121 agagcgcgat tgggaacaag cggatcgtgc cgcgcacgcg ccgcggcagc ggcgcgcaca
     9181 cttcatttac gctgatctcc aaggcatctc cactccatcg catctcgctt ggaccgatcg
     9241 acaaccgcga ggtttcgcgc cgtaactgcg ccgcgccgcg ctcggtcatg gtccagcggt
     9301 tcgcgcgctt cccatacagc gccac
//
LOCUS       DASWZT010000581         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_58046, whole genome shotgun sequence.
ACCESSION   DASWZT010000581 DASWZT010000000
VERSION     DASWZT010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58046"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..705
                     /locus_tag="VGE92_16640"
     CDS             154..705
                     /locus_tag="VGE92_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY1315513.1"
                     /translation="MHVSPSGFPALVLNADFRPLSYYPLSLWSWQDAIKAVFLERVNI
                     VANYDQAVRSPSLEIKLPSVVSLKTFVKPSTHPAFTRFNVFLRDRFSCQYCGDREDLT
                     FDHLLPRSRGGHTTWINVVTACSPCNLRKGNLTPPEAKMWPSQMPFQPSVQHLHRNGR
                     AFPPNYLHESWLDYLYWDTELEP"
     gene            741..1571
                     /locus_tag="VGE92_16645"
     CDS             741..1571
                     /locus_tag="VGE92_16645"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY1315514.1"
                     /translation="MANTLPIHRGVAVLAVVLAAWVVAMQAASAQQVPASAGPPDPKL
                     IEDLVAANRILADQGLLDGWGHVSVRHDKDPNRFLMARGVAAQLVTPQDIIEFDLDSR
                     PVDTHGLPMSALFTERYIHGEIYKLRPDVIAVVHTHAPSLIAFGDTKVPLQPMYHRSA
                     FIAFGVPVFEIRERAGMTDMLIRNQTLGRNLAESLGNHPAVLMRGHGAAITGPSLPRV
                     VSRTIFLSLNATLQADAMRMGAPITYMDIEEARLIEEREGHGLARTWEGWKQKAMGKP
                     "
     gene            1806..>2020
                     /locus_tag="VGE92_16650"
     CDS             1806..>2020
                     /locus_tag="VGE92_16650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315515.1"
                     /translation="MNKPVGTGATRIEPVTPVVLVPKAPPVAPAAPISSRIRKSWLIA
                     GAASLAVLAGVGIVWWLSSANGSVSYTT"
BASE COUNT          347 a          660 c          614 g          399 t
ORIGIN      
        1 atggcgggga gtttttggcc aagcagtttg cccgccgcgc ataatgggta accgcccgcc
       61 gctacttcac aatcccgtca cgctgcccta ttactaggcg gcaagctgcg tgacggcagc
      121 catagggagg ttttagagga gcctcgcttg aacgtgcatg tatctcccag cgggtttccg
      181 gcgctggtgc tcaacgcgga tttccggccg ctgagctatt accccttgtc gctgtggtcc
      241 tggcaggacg cgatcaaggc ggtttttctc gagcgcgtga acattgtcgc caattacgac
      301 caggcggtgc gcagcccgag cctcgagatc aagctgccga gcgtggtgtc gctcaagact
      361 ttcgtaaagc catcgacgca tccggccttc acgcggttca acgtgttcct gcgcgaccgt
      421 ttctcctgcc aatattgcgg cgaccgcgaa gacctgacct tcgatcacct gctgccgcgc
      481 tcgcgcggcg ggcacacgac gtggatcaac gtcgtcaccg cctgctcgcc ctgcaatctg
      541 cgtaagggca atctgacgcc gcccgaggcc aagatgtggc cctcgcagat gccattccag
      601 ccctcggtcc agcatctgca tcgcaacggg cgcgcgttcc cgccgaacta tctgcacgaa
      661 agctggctcg actatctcta ttgggatacc gaactggagc cgtgaggcgc cactcgtttt
      721 tctgcgcatc ggagtttacc atggccaata cgctgcctat tcatcgcggc gttgccgtat
      781 tggctgtcgt tctcgcagcc tgggtcgtgg cgatgcaggc ggcatccgcg cagcaagtgc
      841 cggcctccgc cggtccgccc gatccgaagc tgatcgagga tctggtcgcc gccaaccgca
      901 tccttgcgga ccagggcctg ctcgacggtt ggggccatgt gtcggtgcgg cacgacaagg
      961 acccgaaccg tttcctgatg gcgcgcgggg tcgcggccca acttgtcacc ccgcaggaca
     1021 ttattgaatt cgatcttgat agccggcccg tcgatacgca cggcttgccg atgtcggcgc
     1081 ttttcaccga gcgctatatc catggcgaga tttacaagct ccgccccgac gtgattgcgg
     1141 tcgtgcacac ccatgcgccc tcgctgatcg cgttcggcga taccaaggtg ccgctgcagc
     1201 cgatgtatca ccgttcggcc tttatcgcct tcggcgtccc cgtgttcgag atccgcgagc
     1261 gcgccggcat gaccgacatg ctgatccgca accagacgct cggccgcaac ctcgccgagt
     1321 cgctcggcaa ccacccggct gtcctgatgc gcggccacgg cgcggcgata acggggccgt
     1381 cgctgccgcg cgtggtgagc cgcacgattt tcctgtcgct caatgccaca ctccaggcgg
     1441 acgccatgcg catgggcgcg ccgataactt atatggacat cgaggaagcg cgcctgatcg
     1501 aggagcgcga gggtcacggc cttgcccgca cctgggaagg ctggaagcag aaggcgatgg
     1561 ggaagcctta gctgcctggc attatgtact gcagcacacg gtcgcacctt cccgcccaac
     1621 taccggcacg atagccgcct cgattgtctt tatcgggata ccgagctgga gccggaagcg
     1681 caggcgccgg caggccgttt tagcccgatt cacttgtctt cttagcgatt tgacacgaat
     1741 caaagccggc ttggcgccgc ccgctagaat gcccgttgct ggggttcgca ggcggaattt
     1801 gcgcgatgaa caaacccgta ggaaccggtg cgacgcgaat tgagccggtc acacccgtgg
     1861 ttctggtccc gaaggcacca ccggttgcgc cggccgcgcc tattagttca cgcatcagaa
     1921 aatcctggct gatcgcgggg gcggcgtcgc tcgccgtgct tgccggtgtc ggaatcgtgt
     1981 ggtggctctc gagcgccaac ggcagcgtca gctacaccac
//
LOCUS       DASWZT010000582         7714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_19259, whole genome shotgun sequence.
ACCESSION   DASWZT010000582 DASWZT010000000
VERSION     DASWZT010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7714
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19259"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VGE92_16655"
     CDS             <1..340
                     /locus_tag="VGE92_16655"
                     /inference="COORDINATES: protein motif:HMM:NF018249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315516.1"
                     /translation="RRLGNHARHWTQLLTQGAGNYCTSQQEDNATLNALTRAARSALV
                     DLQRVAVLRTASDFDRPYPKQSAFQSLRAQRALPGAIRASTDNLLRAAMPLVEEISVH
                     WDRWAGGVPP"
     gene            357..911
                     /locus_tag="VGE92_16660"
     CDS             357..911
                     /locus_tag="VGE92_16660"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEY1315517.1"
                     /translation="MEKLFISADSLLSDSMELARRVVSSGFRPTFLVAMWRGGAPIGI
                     AIQEVLEYHSIVADHIAIRTSSYTGIDNQSKTVRVHAVDYLVSRLSAEDELLLVDDVF
                     DSGRSLEAVISELKQRCRRNWPERFRIATVYYKPSRNRTTFVPDYYVLATDKWLVFPH
                     EIVGLTREEILEHKPVGPNFFDKP"
     gene            complement(931..1422)
                     /locus_tag="VGE92_16665"
     CDS             complement(931..1422)
                     /locus_tag="VGE92_16665"
                     /inference="COORDINATES: protein motif:HMM:NF016213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEY1315518.1"
                     /translation="MHSVWLVLGAMLIIAGLAGAIIPAVPGIPLIFGGICLIAALDHY
                     QHLGLWWLIGIAIVGAIGLTFDLLASALGAKRAGASKQAVAGALLGTLIGLFFGLPGL
                     LLGPFAGAVLGELASGNSMSRSTHVGVSAWIGLLFGTILKLVASLMMVALFGAAWWWN
                     RSP"
     gene            complement(1451..2542)
                     /locus_tag="VGE92_16670"
     CDS             complement(1451..2542)
                     /locus_tag="VGE92_16670"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY1315519.1"
                     /translation="MKHHPNPLRAAAWLAAALILGAVPAHAADEPVKVAFVYVDPVGD
                     TGWSYEHDLGRKELERVLGAKVKTTYVESVPANADAERVIRQLAVAGNQLIFTTSFGY
                     MEPTLRVAKLFPKVHFEHASGYKAAANVVTYESRFYEGAYLLGVLAGKTTKSNTLGYV
                     GSFAIPEVIRNIDAFTLGARSVNPKITTKVIWVDTWYDPGKERQAAEALIAQGADVLA
                     QNTDSPAIVQVAESRGVHAFGWDSDMAKYGPKAQITANTENWGDYYIDEVRKVMAGTW
                     SGNRHIAYGLKENMVVLSPLNKSVPPDVVKLFEEKKQGIIDGKLVPFAGPIKDNTGVA
                     KVPAGTALTYEQLLALNWYVEGVDGSIPK"
     gene            2732..3085
                     /gene="uraH"
                     /locus_tag="VGE92_16675"
     CDS             2732..3085
                     /gene="uraH"
                     /locus_tag="VGE92_16675"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR02962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEY1315520.1"
                     /translation="MGKLTTHVLNVAAGIPAAGMRIELCTESHGTMRLTGTAHTNEDG
                     RCPAPLIEGPAFCAGRYSLTFHVAAYFRSLGISLAEPPFLDQVVIAFGIADAQQNHHV
                     PLLVSPWSYSTYRGS"
     gene            3085..4572
                     /gene="xdhA"
                     /locus_tag="VGE92_16680"
     CDS             3085..4572
                     /gene="xdhA"
                     /locus_tag="VGE92_16680"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HEY1315521.1"
                     /translation="MAGSSHTLRFMLDGARVSVTEPHPTTTVLDYLRDTGRRGTKEGC
                     AEGDCGACTVVLGERDAANRRVIYRAVNSCIRFLPTLDGRELITVESLRQPSGELHPV
                     QQSMVDNHASQCGFCTPGFVMSLFALYLNRSGAEREHVLEALAGNLCRCTGYRPIIDA
                     GMRQWSYPAPLNWSREESQSAARLEGLDSVSRDGGRGASLDYAGYWAPRSLEELAGAL
                     DAQPDAVLLAGGTDVGLWVTKQLRELPALVYLGDVPELSRIEARAHELMIGAAVTLTD
                     AWPALTQRFPALQEQAARFASPPIRNSATLCGNLANGSPIGDSIPVMLALGAELELRR
                     GADVRRVALEDFYLGYMKNALRKGEFVSAVAIPLPADAQVLASYKVSKRIDQDISAVC
                     ATFCIRVAGEQVLGARLAFGGLAGIASRAHHAERALAAHGWSLAGVEAASAALASDFK
                     PLSDLRASSAYRLRAAGNLLRRCFLEAQRKPIAMRTHDALQGAPP"
     gene            4569..6863
                     /gene="xdhB"
                     /locus_tag="VGE92_16685"
     CDS             4569..6863
                     /gene="xdhB"
                     /locus_tag="VGE92_16685"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HEY1315522.1"
                     /translation="MTVGTAPVHDSAALHVSGRAQYCDDIALPANALHAAFGVSPIAH
                     ARINRLDLTAVLATSGVVAIALPADVPGENNYGGAVHDDPIFAQELVQYAGQPLFAVA
                     AESMRAARIAAKRAIFDLAVLPALIDIRAALAAESYVLPSQSMRRGDAQAELARAPRR
                     LRSTVAMGGQDHFYLEGHIAVALPQEDGGMLVHSSTQHPTEVQAIVAHALGLSVNRVI
                     VQCRRMGGGFGGKESQAALIAAAAAILAGKTRRPVKLRLDRDADMLITGKRHDFIADY
                     DVGFDEAGRVLALSVMLASRCGYSADLSGPVNDRAMFHIDNAYYLEHVEIVSHRCKTH
                     TVSNTAFRGFGGPQGMLVIEGILDDIARTLHKDALDVRRANLYGTDQRNVTHYGQVVE
                     HNILPQMLDRLEESSEYRQRRAAVSQWNAAQPIVKRGLALTPVKFGIAFTSTLFNQAG
                     ALLQVYTDGTVLLNHGGTEMGQGLHVKVAQVVAAELGVPLSNIRITATDTSKVPNTSA
                     TAASSGADLNAKAAQAAAITIRDRLAELAATQHGVAAHEIRFADGFVHAGAKSLTFAA
                     MARSAHEARVSLSATGYYRTPKIHWNKDTKIGRPFFYFAFGAAVAEVAVDTLTGETEL
                     LRVDILHDVGASLNPAIDLGQIEGGFLQGVGWLTSEELYWSEAGDLRTHAPSTYKIPT
                     VRDWPAAARIELLPAAPNAEESIYRSKAVGEPPLMLALSVFHAIRDACAACGPAGSMP
                     HLPAPATPEAVLRAIDLLSAGRAA"
     gene            6860..>7714
                     /gene="xdhC"
                     /locus_tag="VGE92_16690"
     CDS             6860..>7714
                     /gene="xdhC"
                     /locus_tag="VGE92_16690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HEY1315523.1"
                     /translation="MSATSRRQRPMAPNIEDSWLKPLTGHWIDSARTLLLQHAAVVRV
                     TVVTLRGSAPREPGASMLIDGAGTVGTIGGGRLEWYATAAARELLRDAGAVPVRIADL
                     TLGTELGQCCGGRVQLWLERLTRDDLPWLTKAARRMRERDGVAITIEFAAGAVSRRLQ
                     RSLAGAHAPALQRSAHRDTLLEIANPERPPLTIFGAGHVGQALVRLLADLALYEITWI
                     DSRAELLPAGLPDGVSARLCVRPADWVDLAPAATRFVVMTHDHALDYELCRRVLARTD
                     GCWLGLIGS"
BASE COUNT         1320 a         2572 c         2463 g         1359 t
ORIGIN      
        1 ccgccgcctc ggcaatcacg cccgccattg gacgcagctg ctcacccagg gagccggcaa
       61 ctactgcacc tcgcagcaag aggacaacgc aacgctcaac gcgctgaccc gcgcagcgcg
      121 atccgcactg gtcgatctgc agcgcgtagc ggtgctgcgc accgcatccg actttgaccg
      181 gccctacccc aagcaaagcg cgttccagtc gctacgcgcg caacgcgcgt tgcccggtgc
      241 gatacgcgca tcgaccgaca atctgctgcg cgcggccatg ccgctggtcg aggagatttc
      301 cgttcattgg gatcgctggg cgggcggcgt ccctccataa atcaaggcat aacaatatgg
      361 aaaaactctt catctcggcc gattcgctct tgagcgactc gatggagctc gcgcggcgcg
      421 tggtgagcag cggctttcgc ccgacgtttt tggtggccat gtggcgcggc ggcgcgccaa
      481 tcgggatcgc gatacaggag gtcctcgagt accactcgat cgtcgccgac cacattgcca
      541 tccgcacctc ctcctacacc gggatcgata atcaaagcaa aacggtgcgc gtgcacgcgg
      601 tcgactatct cgtgtcgcgc ttgagcgccg aggacgagct gctgctggtc gacgatgtct
      661 tcgattccgg ccgcagcctc gaagcggtga tcagcgaatt gaagcagcgc tgccgccgca
      721 attggccgga gcgatttcgg atcgcgacgg tgtactacaa gccgtcgcgt aaccgcacga
      781 cgttcgtgcc cgactactac gtgttggcca ccgataaatg gctggttttc ccccacgaga
      841 tcgtcggctt gacgcgtgag gaaattctcg agcacaagcc ggtgggcccg aacttcttcg
      901 acaaaccttg agcacttggc gcgcgatact tcagggactg cgattccacc accaagcggc
      961 cccgaacagc gccaccatca tgagcgaagc caccagcttg aggatggtac cgaatagcaa
     1021 tccgatccat gcgctcaccc ccacgtgcgt cgagcgcgac atgctgttgc cggaggcgag
     1081 ttcgccgagc acggctcccg caaaaggccc gagcaagagg cccggcagcc cgaagaacag
     1141 tccgatgagc gtgccgagca gcgcacccgc cacggcttgc ttgctggcac ccgcgcgttt
     1201 cgcgccgaga gcgctcgcca gcaaatcgaa cgtgagccca atggcaccga cgatggcaat
     1261 gccgatgagc caccacaggc ccagatgctg atagtgatcg agtgcggcga tcaagcagat
     1321 tccgccgaaa atcaatggaa tgcccggcac cgcgggtatg atcgcaccgg caagaccggc
     1381 gatgatcagc atggcaccga gcacgagcca gacgctatgc attgctcacc tcaaccggct
     1441 tgtcaggcgg ctacttcgga atggagccgt cgacgccctc gacataccag ttgagggcca
     1501 ggagctgctc atacgtcagg gccgtgcccg ccgggacttt cgcgactccc gtgttgtcct
     1561 tgatgggacc ggcaaacggc accaacttgc cgtcgatgat gccctgcttc ttctcttcga
     1621 acaatttgac gacgtcgggc ggcacggact tgttgagcgg cgacaggacc accatgttct
     1681 ccttcagccc gtaggcaatg tggcggttcc cggaccacgt gccggccatg acttttcgaa
     1741 cctcatcgat gtagtaatcc ccccagtttt cggtattcgc cgtgatctgc gccttggggc
     1801 cgtacttggc catgtccgaa tcccaaccga aggcatgcac accgcgcgat tcggccacct
     1861 gcacgatggc cggcgagtcg gtattttgcg cgagcacgtc cgcgccctgc gcgatcaagg
     1921 cctcggccgc ctgccgttcc ttgccggggt cgtaccaggt atcgacccag atgaccttgg
     1981 tggtgatctt gggattcacg ctgcgcgcgc ccaaggtgaa cgcatcgatg ttgcggatca
     2041 cttccgggat ggcaaacgag cccacatagc ccagcgtatt cgatttcgtg gtcttgcccg
     2101 ccagcacccc gagcaagtag gcgccttcgt agaatcggga ttcataggtc acgacgttgg
     2161 cggcggcctt gtagcccgac gcgtgctcga aatgcacttt cggaaacaac ttggccacac
     2221 gcaaggtggg ctccatgtag ccgaacgagg tggtgaagat gagttgattg ccggccacgg
     2281 ccagctgccg gatcacgcgt tccgcatccg cgttggcggg tacgctttcc acgtaggtgg
     2341 tcttgacctt ggcacccagc acccgctcca attccttgcg ccccagatca tgttcgtaag
     2401 accagcccgt gtcgcccacc gggtcgacgt agacgaacgc cactttgacg ggttcatccg
     2461 cggcgtgcgc cggcacggca cctaggatga gcgctgcggc gagccatgcc gcggcgcgta
     2521 gcgggttcgg gtggtgtttc atcggcgagc ctcgagttgt tctaaggtga gaatgcacaa
     2581 gcgtgccgtg caacgcaact tgagcgcgac gcgcgcagcc atagcggccc tttcttgggg
     2641 catcgcgtcg tggcagtgca cacgattagt gcggcagatt gtgtacaaaa atccggcgga
     2701 cgtgctgtaa tgatccgcga cgcgaggtgc gatgggcaaa ctgaccacac atgtgttgaa
     2761 cgtggcggcg ggaattccgg cggccggtat gcgcatcgag ctgtgcaccg agtcccacgg
     2821 cacgatgcgg ctcaccggca ccgcacacac caacgaggat ggccgctgcc ctgcgccgct
     2881 cattgagggg cctgcgtttt gcgccggccg ctactcgctc acctttcatg tcgccgcgta
     2941 cttccgctcg ctcggcattt ccttggctga accgccgttc ttggatcaag tcgtgattgc
     3001 cttcggcatc gccgatgcgc aacagaacca ccatgtgccc ctgctggtct cgccctggag
     3061 ttactcgacc tatcggggga gttgatggct ggctcctcac acactctgcg cttcatgctc
     3121 gacggcgcgc gcgtctcggt gacagagccg cacccgacca cgacggtgct cgactatttg
     3181 cgcgacaccg gccggcgcgg caccaaggaa ggctgtgccg agggagattg cggcgcctgc
     3241 accgtggtgt tgggggaacg ggacgcggcg aatcgacgcg tcatctaccg cgcggtcaac
     3301 tcctgcattc gcttcctgcc gaccttggac ggcagggagt tgatcaccgt ggaaagcctg
     3361 cggcagccct cgggcgagct gcacccggtg cagcaatcga tggtcgacaa ccacgcctcg
     3421 cagtgcggct tctgcacgcc gggattcgtg atgtcgctgt ttgcgctgta cttgaatcgc
     3481 agcggcgcgg aacgtgaaca cgtgctcgaa gcactcgccg gaaacttgtg ccgctgtacc
     3541 ggttatcggc cgatcatcga tgccggaatg cgccaatgga gctatcctgc gccgctcaac
     3601 tggagccgcg aggaatcgca gtccgccgcg cgcctcgagg gcctcgacag tgtctcgcgc
     3661 gatggcggcc gcggcgcatc gctcgactat gcgggctatt gggcgccgcg ctcgctcgag
     3721 gaactcgccg gtgcgctcga cgcgcagccc gatgcggtgc tcctcgccgg cggtacggat
     3781 gtgggcctct gggtgacgaa gcagctgcgc gagttgccgg cgctcgtgta tctcggggat
     3841 gtgcccgagc tgtcgcgcat cgaggcgcgc gcacatgaac ttatgatcgg cgcggccgtc
     3901 accctcaccg atgcatggcc ggccctgacg cagcgctttc ccgcgctcca ggaacaggcg
     3961 gcccgctttg cctctccccc gatccgcaat tccgcgaccc tgtgcggcaa ccttgccaat
     4021 ggctcgccga tcggcgattc gattcccgtc atgctcgcgc tcggcgccga actcgaattg
     4081 cggcgcggcg ccgacgtgcg ccgcgtagcg ctcgaggatt tctacttggg atacatgaag
     4141 aatgcgctgc gcaagggcga attcgtcagc gcagtcgcga tcccattgcc cgccgacgca
     4201 caggtcctcg cgagttacaa ggtgtcgaag cgcatcgatc aggatatctc ggcggtgtgc
     4261 gccacgtttt gcatccgcgt ggcgggtgag caggtcctcg gcgcccgtct ggcgtttggc
     4321 ggcctcgcgg gcatcgcgag tcgcgcgcac catgcggagc gcgcgctcgc cgcgcacggc
     4381 tggagcttgg cgggcgtcga ggcggcgagc gcggcgctcg cgagcgattt caagccgctg
     4441 tctgatttac gcgcgagcag cgcctatcgg ttgcgcgcgg cgggcaattt gctgcgccgt
     4501 tgtttcctcg aagcgcagcg caagcccatc gcgatgcgca cccacgatgc cctgcaaggt
     4561 gcgccgccat gacggtcggc acggcgccgg ttcacgacag cgccgcgctg catgtcagcg
     4621 gccgcgcgca atactgcgac gatatcgccc tgcccgcgaa cgcactgcac gctgcttttg
     4681 gggtgagtcc catcgcgcat gcacgcatca accggctcga tctgaccgcc gtgcttgcca
     4741 cctccggcgt cgtggcgatc gcgctgcccg cggacgttcc gggcgagaac aactacggcg
     4801 gtgcggttca cgacgatccg atctttgccc aagagctggt gcagtacgcc ggccagccgc
     4861 tgttcgccgt tgccgccgag tcgatgcgcg ccgcgcgcat cgccgccaaa cgcgccatat
     4921 tcgatctggc cgtgctgcct gcgctcatcg acatccgcgc agccctcgcc gccgagagtt
     4981 acgtgctgcc gagccagagc atgcgccgcg gcgatgcgca ggccgagctc gcgcgagcac
     5041 cgcggcgtct gcgttcgacc gtggccatgg gcggccagga tcacttctac cttgaaggac
     5101 atatcgcagt tgcgctgccg caggaagacg gcggcatgct ggtgcacagc tcgacgcagc
     5161 accccaccga agttcaggcc atcgtcgcgc atgccttggg actgtcggtc aatcgagtca
     5221 tcgtgcagtg ccggcgcatg ggcggcggct tcggcggcaa ggagagtcag gccgcgctga
     5281 ttgccgccgc cgccgcgatc ttggcgggga aaacccggcg gcccgtgaaa ctgcgcctcg
     5341 atcgcgacgc cgacatgctc atcacgggaa agcggcacga cttcatcgcc gactacgacg
     5401 tcggcttcga cgaggccggc cgggtgctcg cgctatcggt catgctggcg tcgcgctgcg
     5461 gatactccgc cgatctttcc gggccggtga acgatcgtgc gatgtttcac atcgataacg
     5521 cctactactt ggaacatgtc gagatcgtct cgcaccgctg caagacccac accgtgtcga
     5581 ataccgcgtt ccgcggattt ggcggcccgc aaggcatgct ggtgatcgag ggcatattgg
     5641 acgacattgc gcgcacgctc cacaaggatg cgctcgatgt gcggcgcgcc aacctgtacg
     5701 gcaccgatca gcgcaacgtc acgcattacg gccaagtcgt cgagcacaac atcctgccgc
     5761 agatgctgga tcgcctcgag gagagcagcg agtaccggca acgccgcgcc gccgtgtcgc
     5821 aatggaacgc cgcgcaaccc atcgtcaagc gcggcttggc gttgacaccg gtcaaatttg
     5881 gaatcgcctt cacctcgaca ttgttcaacc aggccggggc gttgcttcag gtctataccg
     5941 acggcaccgt gctcctcaac cacggcggca cggagatggg ccagggattg cacgtcaagg
     6001 tggcgcaggt cgtcgccgcg gagttgggag tgccgctgtc gaatatccga atcaccgcga
     6061 cggataccag caaggtaccg aatacctccg ccacggccgc ctcatcgggc gccgacttga
     6121 acgccaaggc ggcgcaggcg gccgcgatca cgattcgcga tcgcctcgcc gagttggccg
     6181 caacgcagca cggggtggcc gcacacgaaa tccgctttgc cgatggcttc gtgcacgcgg
     6241 gtgcgaaatc cctgacgttt gccgccatgg cgagaagtgc ccacgaagcg cgcgtgtccc
     6301 tgtccgcaac gggttactac cgtaccccga agatccattg gaacaaggac accaaaatcg
     6361 gcaggccttt tttctacttt gccttcggcg ccgccgtggc cgaagtggcg gtcgacacgc
     6421 tcacgggtga aaccgagctg ttacgcgtcg acatcttgca tgacgtcggc gcctcgctga
     6481 acccggccat cgacttgggc caaatcgaag gcggtttcct gcagggcgtc ggttggctca
     6541 ccagcgaaga gctgtattgg agcgaggcgg gagatctgcg cacgcatgcg ccctcgacct
     6601 acaagattcc caccgtgcgc gactggcccg ccgctgcgcg gatcgagctc ctgccggcag
     6661 cgcccaatgc cgaggaatcc atttaccgct ccaaggcggt gggcgaaccg ccgttgatgc
     6721 tggcgctgtc ggtgttccat gccatccgcg atgcctgcgc cgcgtgcggg cccgcgggca
     6781 gcatgcctca cctgccggcg cccgcgacgc ccgaagcggt gctgcgcgcc atcgatctgc
     6841 tgagcgccgg gcgcgccgca tgagtgccac ctcacggcgg caacggccga tggcgccgaa
     6901 catcgaggac tcctggctca agccgctcac cggtcattgg atcgacagcg cacgcacgtt
     6961 gcttctgcaa cacgcggccg tggtgcgggt caccgtggtg acgttgcgcg gatcggcgcc
     7021 gcgcgaaccg ggcgcgagca tgctcatcga cggcgccggc accgtgggca ccatcggtgg
     7081 cggccggctc gagtggtacg ccactgcggc ggcacgcgaa ctgctgcgcg acgcgggcgc
     7141 cgtcccggtg cgtatcgccg acctcacctt gggaaccgag cttggacagt gctgcggcgg
     7201 gcgcgtgcaa ctgtggctcg aacggctcac gcgcgatgac ttgccgtggc tgacgaaagc
     7261 ggcgcgccgg atgcgcgaac gcgacggcgt tgccatcacc atcgagttcg ccgcgggcgc
     7321 ggtctcgcgt cgactgcaac gctcgctcgc gggcgcgcat gcgcccgccc tgcagcgatc
     7381 ggcgcaccgc gacaccttgc tcgagatcgc gaatcccgag cgtccgccgc tgacgatttt
     7441 cggtgccgga cacgtgggcc aggcgctggt gcggctgttg gcggacctcg cgctctacga
     7501 gatcacctgg atcgattctc gcgccgaact gctacccgcg ggattgccgg acggcgttag
     7561 cgcgcggctt tgcgtgcggc cggccgactg ggtcgacttg gcgcccgctg ccacgcgatt
     7621 cgtggtcatg actcacgatc acgcactcga ctatgaactg tgccgcaggg tcctcgcccg
     7681 cacggatggc tgctggctcg ggctcatcgg ctcc
//
LOCUS       DASWZT010000583         1616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_53238, whole genome shotgun sequence.
ACCESSION   DASWZT010000583 DASWZT010000000
VERSION     DASWZT010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53238"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VGE92_16695"
     CDS             <1..533
                     /locus_tag="VGE92_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315524.1"
                     /translation="YVLDTSSASLDLANSTVGAPVIVTGFTSLFGAAAPNFTASTLLD
                     PTTIQAELVVDWGSGTAAPFTAFTSSEIDIDAANLSIGPRHEIQVGAQSLNAVGLGSD
                     PLIIPAPTTSDTVFTIGHGVSSTTENFNTYAAFIAQLQTELTGSALATGLTAVGQYTA
                     STFTLSATSITVFLNN"
     gene            560..>1616
                     /locus_tag="VGE92_16700"
     CDS             560..>1616
                     /locus_tag="VGE92_16700"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEY1315525.1"
                     /translation="MPDTRITRRDLLKAASLLAAPVGFARAANPAVQQAASVYAYVGT
                     YTPNGGGIYLFRVDPVSGAMTQLRVFDDVQNPTWLALNPQRTRLYAASEIDNYQGTQN
                     GAVVSYAIDGETHELKRLGAVSSAGATPAHLSVHPSGKFVFVANYGGGNVAVFPVGAD
                     GALGEASDVRPSVGARHRARAADDPPGQFANSDHDGPHMHMVATDPSGQFVVANDAGL
                     DLTLVWRLDALAGRLLPADVPVITAPSGAAPRHFVFHPNGRIFYNLYEHDAKVAVYDY
                     DAAKGSFKAKQLISALPPKFGGSNLSAEILIAADGRFLYVSNRLHNAIAIFAVANDGL
                     LRNISEVWVHADYPRCLT"
BASE COUNT          299 a          518 c          496 g          303 t
ORIGIN      
        1 actatgtgct cgataccagc agcgcctcat tggacctggc caattcgacg gtgggagcgc
       61 cggtcatcgt gaccgggttc acgagtttgt tcggtgcggc ggcgccgaat ttcacggcgt
      121 ccacactgct cgacccgacg acgattcagg cggaattggt ggtcgattgg ggcagcggta
      181 ccgccgcacc gttcaccgcg ttcacgagtt cggagatcga tatcgacgct gcgaacttga
      241 gcatcggtcc gcggcatgaa atccaagtgg gcgcgcaatc gctcaatgcc gtcgggttgg
      301 gatcggatcc gttgatcatc ccggcgccga cgacctcgga cacggtattc accatcggcc
      361 atggcgtgag ctcgaccacc gagaatttca atacctacgc ggccttcatc gctcagctgc
      421 aaacggaatt gaccggctcc gcgttggcga ccggattgac cgcggttgga caatacaccg
      481 cctcgacgtt tacgctctcg gcaaccagca tcacggtctt cctgaacaac tgatgccttg
      541 ctgaacctgt aaacacgcgt tgccagacac aagaatcacc cgtcgagatc tcctcaaggc
      601 agcctcgctg ctcgccgcac ccgtgggttt tgcgcgcgcc gcgaatccag ccgtgcagca
      661 agctgcatcg gtctacgcct acgtaggcac gtacacgccg aacggcggcg gcatctatct
      721 gttccgggtg gatccggtct ccggcgccat gacgcagctg cgggtattcg acgatgtgca
      781 aaacccgacc tggcttgcgc tcaatccgca gcgcacacgc ttgtacgccg ccagcgaaat
      841 cgacaactac caggggacgc agaacggcgc cgtggtgtcc tatgcgatcg acggcgaaac
      901 gcatgagctc aagcggttgg gcgccgtcag ttcagccggc gcgacgcccg cgcacttgag
      961 cgtgcaccca tccggaaagt tcgtgttcgt ggccaattac ggcggtggca atgtggccgt
     1021 ctttcccgtc ggtgcggacg gtgccttggg cgaggccagc gacgtgcggc cgagcgtcgg
     1081 tgcgcgccat cgggcgcgcg ccgccgacga tccaccgggc caattcgcga acagcgacca
     1141 cgacggcccg cacatgcaca tggtcgccac ggatccgagc gggcaattcg tggttgcgaa
     1201 cgacgccgga ctcgatctga cactggtctg gcgcctcgat gcgctggccg ggaggttgtt
     1261 gccggccgac gtaccggtga tcaccgcgcc gtccggtgcc gcaccgcggc acttcgtgtt
     1321 tcatcccaac ggccgaattt tctacaatct gtacgagcac gatgcgaagg ttgcggtcta
     1381 cgactacgat gctgcgaagg gcagcttcaa agccaagcag ctgatatcgg cgctgccgcc
     1441 gaagttcggc ggcagcaatc tatcggcgga gattctcatc gccgccgacg gccgctttct
     1501 ctatgtgtcg aatcggttac acaacgccat cgcaatcttc gccgtggcga acgacggtct
     1561 gctgcgaaat atttcggagg tgtgggtgca cgccgactat ccgcgctgcc tgacgc
//
LOCUS       DASWZT010000584         6896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_77338, whole genome shotgun sequence.
ACCESSION   DASWZT010000584 DASWZT010000000
VERSION     DASWZT010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6896
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77338"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1600
                     /locus_tag="VGE92_16705"
     CDS             <1..1600
                     /locus_tag="VGE92_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315526.1"
                     /translation="FYRANTRGNSRILILDNHDDFGGHAKRNEFDLNGALHLLNGGTL
                     EIDSPRPYGPIAAGLLTTLGIDVPKLAKQTQHLEFYDHQGLQSGVFFDQETFGADKLA
                     VGLGTLPLPQFLAQTPLGESARTTIARIQEGAVDYLPGLSSDDKKERLSRISYEAFLR
                     DLVHAEPAVLHFYHARTMGEWGVGTDAVSALDCWGFGLPGFQGMKLAKGSIRRMGFTP
                     AGYADTGGSFRLHFPDGNATIARLLVRNLVPDAVPGVSAEDVVTARVNYSQLDREASA
                     VRLRLNSIAIRARHLGDPKSAAKVEVTYLRGGRAFTVRARVTVLACYNTMIPYLCPEL
                     PAPQKDALHKLVKTPLVYTSVALRNRRAFDALKVHRIYAPSGYHTYFHLNPHVNIGSY
                     HSPRSASEPILLHMVRTPCKPGLTEHEQNRAGRSELLTATFETFERNIRAQLARTLGA
                     GGFDPARDITAITVNRWPHGYAPEYNPLFDPDVPEAERPHVIGRAQFGRIAIANSDSG
                     GGAYTDSAINQGHRAVMELSRLYR"
     gene            1850..2167
                     /locus_tag="VGE92_16710"
     CDS             1850..2167
                     /locus_tag="VGE92_16710"
                     /inference="COORDINATES: protein motif:HMM:NF040598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpp/OprI family alanine-zipper lipoprotein"
                     /protein_id="HEY1315527.1"
                     /translation="MTLTTTLKASLAVACVVAFAGCTDLKPIQAQIDDLKSQVGKLQG
                     DTAKASSDAAAANSAAAAAQSAASGAQSTANQALSTSQSNSTAIEAINEKIDRMFKKS
                     VSK"
     gene            complement(2239..3204)
                     /locus_tag="VGE92_16715"
     CDS             complement(2239..3204)
                     /locus_tag="VGE92_16715"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007302405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY1315528.1"
                     /translation="MYSRIAGTGSYLPSKILTNADLERLVDTSDEWIRTRTGIEQRHV
                     AAEGETTTDLAEHAARRAMQDACITAADIDLICVGTTTPDLVFPNVGTLLQDRLGIHG
                     CPAFSLEAACTGFVYALSVADKFVRLGESKCALVVGAETLTRIVDWNDRGTCVLFADG
                     AGAVVLKPSPEPGIISTRLHSDGKYKDLLLYPDGVSKGFKLIREGKAGVQMKGNEVYK
                     VAVNTLGALVTETLNANGISKDQLDWLIPHQANIRIIEAIAKRLDLPMERVIVTINTQ
                     GNTSAASVPLALDTGIRDGRVKRGQLLLLEAFGGGFTWGSALIRY"
     gene            complement(3214..4224)
                     /gene="plsX"
                     /locus_tag="VGE92_16720"
     CDS             complement(3214..4224)
                     /gene="plsX"
                     /locus_tag="VGE92_16720"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEY1315529.1"
                     /translation="MSALLHIAIDAMSGDRGASISVPAAVAAAQEFSDVRFTLIGRQA
                     ELERELGSAVPANVYCLHAADVVEMSDHPRDALRRKKDSSMRRAIDLVKSNEAHACVS
                     AGNTGALMATAHFVLKMLPGVERPAIVSLIPSRGGHTYMLDLGANASCTPEQLSQFAV
                     MGSVLAADLQGAHDRPRVGLLNIGAEEIKGNDIVQAAHNLLTASDLNYVGFVEGDDIF
                     SDKVDVVVTDGFTGNVALKAMEGAARLIADALREEFSRTALRKLGYLAARPALKALRT
                     RIDPRRYNGASMVGLNGIVIKSHGGTDLYGFQRAIEVAVLEARSGVPARIAQRLGASR
                     GV"
     gene            complement(4277..4474)
                     /gene="rpmF"
                     /locus_tag="VGE92_16725"
     CDS             complement(4277..4474)
                     /gene="rpmF"
                     /locus_tag="VGE92_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEY1315530.1"
                     /translation="MAVQKSRKTPSKRGMHRSHSALPLPAVSIDAKSGETHSRHRISR
                     EGFYRGRKVLQTKADAAAAEE"
     gene            complement(4490..5092)
                     /locus_tag="VGE92_16730"
     CDS             complement(4490..5092)
                     /locus_tag="VGE92_16730"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HEY1315531.1"
                     /translation="MEVEAKISTGGDFKNNDLHPIHFDRGGNQPYYTRPMAPGLPDRV
                     DCAHLADHAVVLERVYPLGEMPRLQDRLADSQGSVRARFAFAKLESGRAIANVAIEAS
                     PHLVCQRCLRGFAYPAAGSSEIEFSSAADAAAVDSEREVYASEGGQVSLRELAEEELL
                     LALPIVALHEPQSCARVTPAFEADERSRPFAGLQDLLKKT"
     gene            5052..5651
                     /locus_tag="VGE92_16735"
     CDS             5052..5651
                     /locus_tag="VGE92_16735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HEY1315532.1"
                     /translation="MKSPPVLILASTSTYRRQLLARLRLPFTCSAPGVDETPRAAERP
                     LALAARLARAKAMSVALQHPDAWVIGSDQVAALGDGAAERTLGKPGTEALCIEQLRGS
                     SGHTVTFLTAVAVVRHDGRAAYQFVDTTRVTFRTLDDDTIARYLAAESPLDCAGGFKS
                     EGLGITLCESIDSVDPSALIGLPLIRLSEVLRSVGFALP"
     gene            complement(5657..6727)
                     /locus_tag="VGE92_16740"
     CDS             complement(5657..6727)
                     /locus_tag="VGE92_16740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY1315533.1"
                     /translation="MNSNEAAPQRPGAPKTPLTLRTITADEAGQRIDNFLMRHFKSVP
                     RSRVYRLLRKGEVRVNRKRVAAEYRIRDGDQVRLPPVRLEVGEEPGRDGPSRPSLSLQ
                     ELIERAVIFQDKHLLVIDKPAGVAVHGGSGMSFGVIETLRASRPRETLELVHRIDRDT
                     SGCLLVARDRATLTALHALIRSGGMHKTYLALVAGSWQLGTKRIDVPLATDNRQHGER
                     HVRVAAAGKDSVSVFKPVQFFGPLATLVEVDIPTGRTHQIRVHASFAGHPLLGDDKYG
                     DRERNAELKQHGLKRTFLHAQSVAFDWPGSGVPFHASAPLPAELAAVLDAITPAKKRA
                     TARRSPPRKVPGAAPAKRPARR"
BASE COUNT         1307 a         2222 c         2044 g         1323 t
ORIGIN      
        1 cttctatcgc gcgaacactc gcggcaacag ccgcatcctc attctcgaca atcacgacga
       61 cttcggtggc cacgccaaac gcaatgagtt cgacctcaac ggcgctctgc atcttctgaa
      121 cggcggcacc ttggaaatcg acagtccgcg accttacggg ccgatcgctg ccggcttgtt
      181 gactacactc ggtatcgatg tgccgaaact cgccaaacag acgcagcacc tcgagttcta
      241 cgaccaccag ggtttgcaga gcggcgtgtt cttcgaccaa gaaacctttg gcgccgacaa
      301 gctggcggtg ggcctcggca cgctgccgct cccgcagttc ttggcgcaaa cgccgctcgg
      361 cgagtccgcg cgcaccacga tcgcgcgaat tcaagaaggc gccgtcgatt acttgcccgg
      421 cctctcatcc gacgacaaga aggagcgtct gtcgcgcatc agctacgaag cgttcctgcg
      481 agacctcgtc cacgccgaac ccgcggtcct gcatttctat cacgcgcgaa ccatgggcga
      541 atggggggtc ggcaccgacg ccgtatctgc cctcgactgt tggggtttcg ggcttcctgg
      601 atttcagggc atgaagctcg caaagggttc gattcggcgc atgggtttta cgccggcggg
      661 atacgccgac acgggcggct cattccgctt acattttcct gacggcaacg cgacgatcgc
      721 ccggctgctg gtaagaaatc tcgtgccgga cgcggtgccc ggcgtgagcg ccgaggacgt
      781 ggtgacggcg cgggtgaatt attcgcaact cgatcgcgag gcgagtgccg ttcgcctgcg
      841 tctcaacagc attgcaatcc gcgcacgcca tctcggcgac cctaaatcgg ccgcgaaggt
      901 cgaagtcacg tatctgcgcg gcgggcgtgc gttcaccgtg cgcgcccgag tcacggtgct
      961 tgcgtgctac aacacgatga ttccctacct gtgcccggaa ctgccggcgc cgcagaaaga
     1021 cgcactgcac aagctggtga aaactccgct ggtctatacc agcgtcgccc tgcgcaaccg
     1081 tcgggccttc gacgcgctca aggttcatcg gatctatgcc ccgagcggct atcacaccta
     1141 ttttcatttg aacccgcacg tcaatatcgg cagctaccac agtccccgct cggcaagcga
     1201 gccgattctt ctgcacatgg tgcgcacccc ctgcaagccg ggattaaccg agcacgagca
     1261 gaaccgcgca gggcgcagcg aactactcac ggccacgttc gagaccttcg aacgcaacat
     1321 ccgcgcacag cttgcgcgca ccctgggcgc cggaggcttc gacccggccc gcgacatcac
     1381 ggcgatcacg gtgaatcgat ggccccatgg ctacgcacct gaatacaatc ctctgttcga
     1441 tccggacgtg cccgaagccg agcggccgca cgtgatcggt cgtgcacagt ttgggcgcat
     1501 cgcgatcgcc aactccgact ctgggggtgg ggcatacacg gattcggcta tcaatcaggg
     1561 gcatcgcgca gtaatggaac tttcgcggtt ataccgctga atccagcggt aatatttaca
     1621 ttccggggcg aattcttaat gatgttttaa tttccacgca ggaaacctca cgtaaaaacg
     1681 tcgccgtgaa ccgacaaata gcgcatttca gttgcattcg aggcccgaat ttggcttcat
     1741 gtgcgactgt gttgcgacgc aggcgggccc gacaccgtga ctgggccagc ttgtgacaac
     1801 acttcggact gttccgatac gaatgaatta acttagggga atattcttta tgacattgac
     1861 caccacacta aaggcctctc tcgccgtggc ctgcgtagtc gcattcgcag gttgcacgga
     1921 tctgaagccc atccaggcac agatcgacga tctgaaatca caagtcggca aactgcaggg
     1981 cgacacggcg aaagcctcga gcgatgcagc tgctgccaac agcgcggctg ccgccgcaca
     2041 atcggccgcg tccggcgcgc agagcactgc gaatcaggcg ttgtcgacct cgcagtcgaa
     2101 ttcgacggcc attgaagcga tcaacgagaa gatcgatcgc atgttcaaga agtcagtgtc
     2161 gaagtaaatc gggcatttgc ctcgaagata cgccgcggtc actccgcggc gttttttttt
     2221 gcgcgggaaa tttgcgcgtc aatagcgaat cagagccgaa ccccaggtga agccgccgcc
     2281 gaacgcctcc aatagcagca gttgtccgcg cttcacgcgg ccatcgcgaa tacccgtatc
     2341 gagagccaaa ggcaccgacg cggcggaggt attgccctgc gtattgatgg tcacgatcac
     2401 ccgttccatc ggtagatcaa ggcgtttggc gatcgcctcg atgattcgaa tgttcgcctg
     2461 atgcgggatc agccagtcga gctgatcctt gctgatcccg ttggcattga gcgtctccgt
     2521 gaccaaagcc ccgagcgtgt tgacggcgac cttgtagact tcgttgccct tcatctggac
     2581 gccggcctta ccctcgcgaa tcaatttgaa gcccttggag actccgtccg ggtacaacaa
     2641 caaatccttg tatttgccgt cggaatgcag gcgcgtgctg atgatccccg gctcgggtga
     2701 gggtttcagc accaccgccc cggcaccgtc cgcgaatagc acgcacgtgc cgcggtcgtt
     2761 ccaatcgacg atgcgcgtca gcgtctcggc gccgaccacg agcgcgcatt tcgactcccc
     2821 caagcgcacg aatttgtccg caacactcag cgcgtagaca aagccggtgc aggcggcttc
     2881 gaggctgaaa gccggacagc catggatgcc caatcggtct tgcaacaagg tgccgacatt
     2941 cggaaacacg agatctggcg tggttgtgcc tacgcaaatc aggtcgatgt ccgcggcggt
     3001 gatgcatgcg tcctgcatcg cgcgccgcgc cgcgtgctcg gcgagatcgg tcgtcgtctc
     3061 gccttcggcg gccacgtgtc gctgttcgat tcccgtgcgc gtacgaatcc attcatcgct
     3121 ggtgtcgacg agtctctcga gatccgcatt ggtaaggatt ttgcttggta ggtaactacc
     3181 ggttccggcg atacgtgagt acatcgaact cccttagacc ccgcgtgaag cgcccagccg
     3241 ttgcgcgatg cgcgccggta cgccgctgcg cgcttcaagt accgcgactt cgatggcgcg
     3301 ctgaaacccg tacaggtcgg tgccgccatg actcttgatg actatcccgt tcaaacctac
     3361 catgctcgcg ccattgtagc ggcgcggatc gatccgcgtg cgcaacgcct tgagggcggg
     3421 ccgcgcagcg aggtagccga gcttgcgcaa cgcggtgcga ctgaactcct cgcgcagcgc
     3481 gtcggcgatc aatcgcgcgg cgccttccat cgctttcagg gccacgttgc cggtgaagcc
     3541 gtcggtgacc acgacatcga ctttatcgga aaaaatatcg tcgccctcga cgaaaccaac
     3601 gtaattaaga tcgctggccg tcaacagatt gtgcgccgcc tgcacgatgt cgttgccttt
     3661 gatctcctcg gcaccaatat tgagcaatcc cacgcgcggc cggtcgtgcg cgccctgcaa
     3721 gtccgccgcc agcaccgatc ccatgacggc aaactgcgaa agctgctccg gcgtgcagga
     3781 tgcattcgcc ccgagatcca acatgtaagt gtgcccgccg cgcgacggga tcagcgaaac
     3841 gatggccggc cgctccacgc cgggaagcat cttcaagacg aagtgcgcgg ttgccatcaa
     3901 cgcaccggta ttgccggcgc tgacgcacgc atgcgcctca ttcgacttga ccaagtcgat
     3961 ggcgcgccgc atcgacgagt ccttcttgcg ccgcaaggca tcgcgcggat gatcgctcat
     4021 ctcaacgaca tcggccgcat gcaggcaata cacattggcg gggactgcac tccccagttc
     4081 gcgctcaagt tcggcttgcc gcccgatcaa ggtaaagcgg acgtcgctga attcctgggc
     4141 cgcggccacg gcggcaggca cggatatgga cgcccctcga tcgccactca tggcatcgat
     4201 ggcaatgtgc agcagcgcag acacggtgcg tcaaataagc ccgagcgccg ggcgaaccgt
     4261 cgccggcact cgcgaattat tcttcggccg ctgccgcgtc cgccttggtc tgaagcacct
     4321 tgcggccgcg atagaacccc tcgcgagaaa ttcggtgacg cgaatgggtt tcgccactct
     4381 tggcgtcgat cgacaccgcg ggcagcggta acgccgaatg cgagcgatgc atgccgcgtt
     4441 tggagggggt ttttctgctt ttttgaactg ccatgggtgc tatgtcctat caagttttct
     4501 ttaacaaatc ctgcaatccg gcaaacgggc gcgaacgctc gtccgcctcg aaagccggcg
     4561 ttaccctagc gcagctctgc ggctcatgca gcgctacgat cggtagtgcc aacaacaatt
     4621 cttcctcggc cagttcgcgc aggctcacct gccctccctc gctcgcatac acttcccgct
     4681 cggagtcaac ggcagccgcg tcggcggcgc tcgaaaactc gatctcgctg ctgccggcgg
     4741 cgggataggc gaagcctcgc aaacaccgct ggcataccag atgcggcgac gcttcgatcg
     4801 cgacgttggc gatcgcccgg cccgactcga gtttcgcaaa agcgaaacgc gctcgtaccg
     4861 acccctgcga gtccgccaag cggtcctgta agcgaggcat ctcaccgagc gggtagacgc
     4921 gctccagcac caccgcatga tcagccaggt gcgcgcagtc gactcggtca ggcaagcccg
     4981 gtgccatggg gcgcgtataa tagggctggt taccacccct gtcaaaatga atcgggtgca
     5041 agtcgttgtt tttgaagtct ccgcccgtac taattttagc ctcgacctcc acttaccggc
     5101 gtcagctcct ggcgcggctg cgcctgccat ttacctgcag cgcgccggga gtcgacgaga
     5161 cgcctcgggc cgccgagcgg ccgctcgccc tggcggcgcg tttagcccgg gccaaggcaa
     5221 tgagcgtggc gctgcagcac ccggatgcct gggtcatcgg ctccgatcaa gtagcggcct
     5281 tgggcgacgg cgcggccgag cggaccctcg gtaagcccgg caccgaggca ctttgtatcg
     5341 aacaattgcg cggcagctcg ggccataccg tcaccttctt gaccgccgtg gcggtcgtgc
     5401 gccacgacgg gcgtgccgcc taccagttcg tggatacaac ccgcgtgaca tttcgcacgc
     5461 tcgacgatga cacgattgcc cgttacttgg cggccgagtc gccgctcgat tgcgccggcg
     5521 gattcaagag cgagggcctc ggaatcacgc tctgcgaatc gatcgacagc gtcgacccga
     5581 gcgccctcat cgggctgccg ttgattcgtt tgagcgaagt gctgcgcagc gtaggcttcg
     5641 ccttgccgtg attcgatcag cgccgagcgg gacgcttggc gggcgcggct cccggcactt
     5701 ttcgcggcgg cgagcggcgc gcagtcgcgc gctttttggc gggcgtaatc gcgtcgagca
     5761 ctgcggccaa ctccgcgggt agaggcgcac tggcgtggaa cggcacgccc gagccgggcc
     5821 agtcgaaggc cacggattgc gcatgcaaga aggtgcgctt gagtccatgc tgtttgagct
     5881 cggcattgcg ctcgcgatcg ccatatttgt catcgccgag cagcggatgg ccggcgaagg
     5941 aggcgtggac gcggatttga tgcgtgcgtc cggtcggaat gtcgacttcc accaaggtcg
     6001 ccaatgggcc gaaaaattgc accggcttga acacactgac cgaatccttg ccggccgcgg
     6061 ccacgcgcac gtgccgctcg ccgtgctggc gattatcggt cgccaacgga acgtcaatgc
     6121 gtttggttcc gagttgccag ctgcccgcaa ccaaggcgag gtaggttttg tgcatgccgc
     6181 cgctgcgaat caacgcatgc agcgccgtga gtgtggcgcg gtcccgcgcc accaacaagc
     6241 aaccgctggt atcgcggtcg atacgatgta cgagctcgag cgtctcgcgc ggccgcgacg
     6301 cccgcaatgt ctcgatcacg ccgaaactca tgccgctgcc gccgtgtacg gcgacgccgg
     6361 ctggtttgtc gatcaccaac agatgcttgt cctgaaagat gaccgcccgt tcgatcaact
     6421 cctgcaagct cagcgacggg cggctcgggc catcacgccc cggctcttcg cccacctcga
     6481 gccgcaccgg cggcagtcgg acctggtcgc catcccgaat tcgatattcc gcggcaacgc
     6541 gcttgcggtt gactcgcacc tcgcccttgc gcaacagccg ataaacccgg ctgcgtggca
     6601 cggacttgaa atgccgcatc agaaaattgt cgattctctg acccgcctca tcggcggtga
     6661 tcgtgcgcag agttaacggc gtcttcggtg cccccgggcg ctgcggcgct gcttcattac
     6721 tgttcatgtt tcacgagatt tcccgggcaa tcccctaagg tattgattac attgaccctg
     6781 gtaggctgcc gtgataatgt cttaaaaccg tgtcaggcgc tgaaccattt ctgggcgaag
     6841 ctcaattctt gggtttgctt aagacaccgg tttcggtggc gcccccgtcc ttaggg
//
LOCUS       DASWZT010000585         5759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_67730, whole genome shotgun sequence.
ACCESSION   DASWZT010000585 DASWZT010000000
VERSION     DASWZT010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67730"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1531)
                     /locus_tag="VGE92_16745"
     CDS             complement(143..1531)
                     /locus_tag="VGE92_16745"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF006563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEY1315534.1"
                     /translation="MNTSFPIRLGSVAPTVRSFSAELEPLRIVETFPAAYPGLLESGA
                     GMAAASSGSRFDILPMANGECLRLGSDGALSGPYAGASGFLSAFEEWWSAQRVPQAPT
                     PLPFTGGWLLYMGYELAAEIEPRLRLPRSNDPVAALAIRAPAAWIRDRASGQAWLLAE
                     PGFEYLLDEFAERVRDLRTATPVGAGAFSIAEDDPEVFIAAVRRGLEYIAAGDVYQAN
                     LSRAWAATASGAADPVAIYRRLRATNPSPFAAFLRFEDFALLSSSPERLVSVRGNTVA
                     TRPIAGTRPRGASAQADAELVRSLLRNEKERAEHVMLIDLERNDLGRVCAAGSVRVDE
                     YMSVESYAHVHHIVSNVSGLLRSDVSPVGVIRALFPGGTITGCPKVRCMEIIAELEAG
                     GRGAYTGSIGYVNRDGNCDLNILIRTITAQGNRFAFRAGAGIVADSKPTHELAETRAK
                     AEGLLRAFAVPS"
     gene            complement(1461..2696)
                     /gene="fabF"
                     /locus_tag="VGE92_16750"
     CDS             complement(1461..2696)
                     /gene="fabF"
                     /locus_tag="VGE92_16750"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY1315535.1"
                     /translation="MRRVAVTGLGIVSPVGNDVASAWAAILAGRSGIAPVARFDTVNF
                     PTHFGGEIRQLDLEPYMSSKDARRMDAFMQYGVVAGMQAMRDSGLTVTEENSDRIGVL
                     MGSGMGGLESIEQTYDKYLETHSPKKVSPFFIPASIINLVSGHLSIAFKMTGPNLAIA
                     TACTTSTHSLGLAMRLIQYGEADAMLAGGSEMATCVTGMSGFAQAKALSQRNDDPQAA
                     SRPWDKDRDGFVMGDGAGAMMLEEYEHAKSRGAKIYAEIVGFGMSGDAFHVTAPPDNG
                     EGARKAMAKALLDAKLNPERVQYVNAHATSTELGDRAETLAIRRAFGAAADKLAVSST
                     KSMTGHLLGAAGAVEAIFSVLALRDQVAPPTINLHTPGEGCDLDYVPNTARRMQLEYV
                     LSNSFGFGGTNGSLIFRRA"
     gene            complement(2738..2980)
                     /gene="acpP"
                     /locus_tag="VGE92_16755"
     CDS             complement(2738..2980)
                     /gene="acpP"
                     /locus_tag="VGE92_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY1315536.1"
                     /translation="MSTVEQQVKKIVAEQLGVKEEEVTNDASFVDDLGADSLDTVELV
                     MALEEEFETEIPDEDAEKITTVQQAIDYINSHKGKA"
     gene            complement(3163..3897)
                     /gene="fabG"
                     /locus_tag="VGE92_16760"
     CDS             complement(3163..3897)
                     /gene="fabG"
                     /locus_tag="VGE92_16760"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEY1315537.1"
                     /translation="MLNAEIALVTGATRGIGAAIAERLARDGARVIGTATTAQGASRI
                     SENLAPFGGRGALLDVLQSASIESLLEDIEAKEGAVAILCNNAGITRDTLLLRMKQED
                     WDAVLDTNLASVFRLSKAVLRGMMKARKGRIVNISSVVGLTGNPGQANYAAAKAGIIG
                     FTKSLAREVGSRGITVNSIAPGFIDTDMTRALSEAQRSALDAQIPLGRLGQPADVAAA
                     VAFLCSADAAYITGETLHVNGGMYMP"
     gene            complement(3908..4852)
                     /gene="fabD"
                     /locus_tag="VGE92_16765"
     CDS             complement(3908..4852)
                     /gene="fabD"
                     /locus_tag="VGE92_16765"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY1315538.1"
                     /translation="MSLAFVFPGQGSQSIGMMSALAKVSPVIGATFAEASAVLGYDLW
                     LRCQDGPPEALNATECTQPAMLTAGVATYRLWRERGGPVPNLMAGHSLGEFTALVAAG
                     ALDFKTAVDVVRYRGELMQAAVPPGEGAMAAILGLEDTDIDTACQEASQGEVVEAVNF
                     NAPGQVVIAGTATAVARAIALATAKGARRALALPISVPAHSSLMEPAAEQLRSRLEVT
                     QFAATPGIAVYGVDIKIHAGADQIRAGLVKQLHTPVYWAATVRTMIAAGATQIVECGP
                     GKVLTGLNRRVDKNRDLKMSALEDPQALDETLASLRHS"
     gene            complement(4865..5482)
                     /gene="rrtA"
                     /locus_tag="VGE92_16770"
     CDS             complement(4865..5482)
                     /gene="rrtA"
                     /locus_tag="VGE92_16770"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhombosortase"
                     /protein_id="HEY1315539.1"
                     /translation="MPADAAKHLAQIAAALQWDRGRWIWLLLILLVLDTVLGLGDSFG
                     QLLRYDRSAIAAGGWWRLLTAHIVHLDLHHLVLNELGLVLVWSLFAQDYDVVEWCAIV
                     LAGALAISSGLWWLSPRVVWYVGASGVLHSVMAAGAAKHLVERTWDRWILIIGLCAKL
                     VWERWGGSQAPLVVTDAHLYGACCGFLVGAALSLRMAIIRRRSRA"
     gene            5529..>5759
                     /gene="gspG"
                     /locus_tag="VGE92_16775"
     CDS             5529..>5759
                     /gene="gspG"
                     /locus_tag="VGE92_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006751354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEY1315540.1"
                     /translation="MHNRQRGFTLIEIMVVVVILSVLGALVVPQIIDKVDVAKVKRAQ
                     SDIRAIQTALDLYRLDNFKYPTTEQGLQALVKQ"
BASE COUNT         1110 a         1886 c         1700 g         1063 t
ORIGIN      
        1 gcctcgagcc gctccaaatg ataggcgagc agccgcaccc ggccggcgcg cacacgcatg
       61 gtttcgaata cgccgtctcc gtactgcaag ccgcgatcgc gtacatcgac ccgcgtgctg
      121 tttcgaccgt tgacccacgt actcatgacg ggacggcaaa tgcgcgcaac aatccttccg
      181 ccttggcgcg cgtctccgcc agttcgtgcg tcggtttcga atccgccacg atgcccgcgc
      241 cggcgcgaaa cgcgaagcga ttgccctgcg ctgtgatggt gcggatcaag atattgaggt
      301 cgcagttgcc gtcgcgattg acatagccga tagatccggt gtaggcgccg cggcccccgg
      361 cttcgagttc ggcaataatt tccatgcatc gaaccttggg acagccggta atcgtcccgc
      421 cgggaaacag ggcgcgtatc acgccgaccg gcgatacgtc gctgcgcaat aatccgctga
      481 cattcgacac gatgtgatgc acgtgcgcgt agctctcgac gctcatgtat tcgtcgacgc
      541 gcaccgaacc cgcggcacag acccggccta agtcgttgcg ctccaagtcg atcaacatga
      601 cgtgttcggc gcgttccttt tcgttgcgca gcagcgagcg aaccagctcc gcgtcggcct
      661 gcgcagaggc gccgcgcggc cgcgtgccgg caatcggccg agttgcaacc gtatttccgc
      721 gaaccgacac cagtcgttcc ggcgaagaac tcagcaaggc aaaatcttcg aagcgcaaga
      781 acgccgcgaa cggactggga ttggtggcac gcaggcgccg gtaaatagcg accggatcag
      841 cggctccgga cgccgtcgcc gcccacgccc gcgacaagtt cgcctgatac acatcgccgg
      901 cggcgatgta ctcgaggcca cggcgcaccg ctgcgatgaa gacctcgggg tcatcctcgg
      961 cgatgctaaa agcaccggcg ccgacgggcg ttgccgtgcg caagtcccgc acgcgctcgg
     1021 caaactcatc gagcaggtat tcgaaccccg gctccgcgag caaccacgcc tggccgctcg
     1081 cccgatcgcg gatccaggct gccggcgcgc gaatcgccaa tgccgccacc gggtcgttgc
     1141 tgcgtggcag ccgcagacgc ggttcgattt cagccgccag ctcatagccc atgtagagaa
     1201 gccagccgcc ggtaaatggc aatggggtcg gcgcctgcgg cacacgctgc gcactccacc
     1261 actcctcaaa ggcgctgaga aagccgctcg cgcccgcata cggtccggac agcgcgccgt
     1321 cgctacctag tcgcaagcac tcgccgttcg ccatcggcaa gatgtcgaaa cggctgccgc
     1381 tgctcgccgc agccatgccg gcaccgcttt cgagcagtcc cggatatgca gccggaaaag
     1441 tctcgacgat gcgcagcggc tcaagctctg cggaaaatga gcgaaccgtt ggtgccaccg
     1501 aacccaaacg aattggaaag gacgtattca agctgcatac gccgcgcggt gttgggaacg
     1561 taatcgaggt cgcagccctc gccgggtgtg tgcaggttga tggtcggcgg tgcgacctga
     1621 tcgcgcagcg ccagcaccga gaagattgcc tcgacggcgc cggcagcgcc cagcagatgg
     1681 ccggtcatcg acttggtgga gctgacagca agtttatcgg ctgcggcgcc gaacgcgcgg
     1741 cgaatcgcca aggtttccgc gcgatcgcct aattcggtcg aggtggcgtg cgcattgacg
     1801 tattgcaccc gctcgggatt tagctttgca tcgagcagcg ccttcgccat ggctttgcgc
     1861 gcaccctcgc cgttatcggg cggagcggtg acgtggaatg catcgccgct catgccgaat
     1921 ccgacgatct cggcatagat cttcgcaccg cgtgacttcg cgtgttcgta ctcctcgagc
     1981 atcatcgcgc cggcgccgtc gcccatgacg aaaccatcgc gatccttgtc ccagggacgg
     2041 ctggcggctt gcggatcgtc attgcgctgt gacagcgcct tcgcctgggc gaaaccgctc
     2101 atgccggtga cacaggtcgc catttccgaa ccaccggcca gcatggcatc ggcttcgccg
     2161 tactgtatga gccgcatggc caggcccagg gagtgcgtcg aggtcgtgca tgcggtggcg
     2221 atggccagat tcggacccgt catcttgaat gcgatcgaca ggtgaccgct caccaaattg
     2281 atgatgctgg cgggaatgaa gaacggcgac accttcttgg gcgaatgcgt ctcgagatac
     2341 ttgtcatagg tttgctcgat cgactcgagc ccccccatcc ccgaacccat gaggacgccg
     2401 atccgatcgg agttctcttc ggtgaccgtc aggccagagt cgcgcatggc ctgcatcccg
     2461 gcgacgacgc cgtactgcat gaaggcatcc atgcggcgcg catccttgct gctcatgtag
     2521 ggctcgagat cgagctgccg gatctcgccg ccgaagtgcg tcgggaagtt gacggtatcg
     2581 aagcgcgcaa ccggtgcgat gccgctgcgg ccggcgagga tcgcggccca cgcgctcgcg
     2641 acatcgttgc ccaccggcga gacgataccg agacccgtta cggcgacgcg tcgcatagtc
     2701 aaccgcgact cctcaggacc ttaagtccca acgggattta ggccttgccc ttgtgcgaat
     2761 tgatatagtc gatggcctgc tgaacggtgg tgattttctc ggcgtcttcg tcgggaattt
     2821 ccgtctcgaa ctcttcctcg agcgccatca ccagctcaac cgtatccagc gagtctgcgc
     2881 ctagatcgtc gacgaacgag gcgtcattgg tgacttcttc ttcttttacg cccaattgtt
     2941 cggccacgat cttcttaacc tgctgctcaa cagtactcat cgttatggtg tcctctcgga
     3001 tttactactt atgcactgaa ctgacttaag cccgcgacca gccttaagga atcgacaggg
     3061 cgcgtatttt atgtgaacgc agcggcggcg gctaccttga agctgcattc tgcccacccc
     3121 ggctgttatt gacaaacagc gcagggtaag ggtgctttga gctcatggca tgtacatgcc
     3181 gccgttcacg tgcaacgtct cacccgtgat gtaggccgca tcggctgaac acagaaacgc
     3241 caccgcggcg gctacgtcgg ccggctggcc gagtcttccc aagggtatct gcgcgtccag
     3301 agcgctgcgc tgtgcctcgc tcaaggcccg ggtcatgtcg gtatcgatga agccgggtgc
     3361 tatcgagttc acggttatgc cgcgcgatcc gacctccctg gcgagcgatt tggtgaaacc
     3421 gatgattccg gccttcgcgg ctgcgtaatt ggcctgtccc gggttgccgg tgaggccgac
     3481 gaccgagctg atgttgacga ttcgaccttt gcgggctttc atcatgcctc gcaatacggc
     3541 cttcgagagc cgaaacaccg aggctaaatt ggtgtcgagc acggcatccc aatcctcctg
     3601 cttcatgcgc aataacaagg tatcgcgcgt gatgccggcg ttgttgcaca ggatcgccac
     3661 ggctccttcc ttcgcttcga tgtcctcgag caagctctcg atcgaggcac tttgcagcac
     3721 atcgagcagc gcgccgcgac cgccgaacgg cgccagattt tcgctgattc ggctcgcgcc
     3781 ctgcgccgtg gtggcagttc cgatcacgcg cgccccatcg cgagcgaggc gctcggcaat
     3841 ggccgcacca ataccgcgcg tggcgccggt caccaaggca atctctgcgt tcagcatgcg
     3901 atgatcctca tgagtgcctc aggcttgcga gcgtctcgtc cagagcctgc ggatcctcga
     3961 gcgcgctcat cttgaggtcg cggttcttat cgactcgccg attcaatccc gtcagcactt
     4021 tgcccggtcc acattccacg atttgcgtcg cacccgcagc gatcatggtg cgcaccgtcg
     4081 ccgcccaata cacgggcgtg tggagctgct tgacgaggcc ggcacgaatt tggtcggcgc
     4141 cggcatgtat cttgatatcg actccataaa ctgcgatacc cggggtagca gcgaattgag
     4201 tgacctcgag ccgggagcgc aactgctccg cggccggctc catcagcgaa ctgtgcgcgg
     4261 gcacgctgat cggcagtgcc aatgcgcgcc gtgcgccctt cgcggtcgcg agcgcaatgg
     4321 cgcgcgccac cgcggtggca gtgccggcaa tgacgacctg gccgggcgca ttgaagttca
     4381 ccgcctcgac tacttcgccc tgcgaggctt cctggcacgc cgtgtcgatg tcggtatcct
     4441 cgagaccgag gatggccgcc atcgcgcctt cacccggcgg cacggccgcc tgcatgagct
     4501 cgccgcggta tctcaccaca tcgaccgcgg tcttgaaatc gagcgcgccc gcggcgacca
     4561 aggcggtgaa ctcgccgagg ctatgtccgg ccatcagatt gggcacgggg ccgccgcgtt
     4621 cacgccacag acgataggtt gcgacgcccg ctgtcaacat ggccggttgt gtgcattcgg
     4681 tggcgttgag cgcttcgggc ggcccgtcct ggcagcgcag ccacaaatcg tagcccaaca
     4741 ccgcggaagc ctcggcgaaa gtcgccccga tcaccggcga caccttcgcc agggcactca
     4801 tcatgccgat ggattgcgag ccttggccgg gaaaaacaaa tgcaagtgac atgactttaa
     4861 gcccctaagc gcgagagcgg cggcgaatta tagccatccg cagagatagc gcagccccga
     4921 ccaggaatcc gcaacacgct ccatatagat gcgcgtcggt gacgaccaac ggcgcttgcg
     4981 atccgcccca ccgttcccag accagcttcg cacacaatcc aatgatcagg atccatcgat
     5041 cccaggtacg ttccaccagg tgtttggcag ccccggcggc catcacggaa tgcagaaccc
     5101 ccgaagcacc gacgtaccaa acaaccctcg gactcaacca ccaaaggccc gaactgatgg
     5161 ccaatgcgcc cgccaacacg attgcgcacc attcgaccac atcgtagtcc tgtgcgaaca
     5221 gcgaccacac cagcaccaag cccaattcgt tgagcactag atggtgcaga tcgagatgca
     5281 cgatgtgggc tgtgagcaac cgccaccagc cgcccgccgc aatcgcgctg cgatcatagc
     5341 gcaggagttg gccgaaactg tcgccaagac ccaacaccgt gtccaaaacc agcaaaatca
     5401 gcagcaacca aatccatcgg ccgcgatccc actgtaatgc cgcggcgatc tgcgccaggt
     5461 gcttcgcggc gtcagccggc atcgatcgcc cctattttgc tataattcaa cgaattctcg
     5521 aggtcactat gcacaatcgc caacgcggtt ttacgcttat cgaaatcatg gtcgtggtgg
     5581 tcattctgtc agtgctgggc gcgctcgtcg tgccccaaat catagataag gtcgacgtcg
     5641 ccaaggtgaa gcgggcgcag agcgacattc gcgcgattca gactgccttg gacctttacc
     5701 ggctcgataa cttcaaatat cccacgaccg aacagggttt gcaggcgttg gtcaagcaa
//
LOCUS       DASWZT010000586        14819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_87070, whole genome shotgun sequence.
ACCESSION   DASWZT010000586 DASWZT010000000
VERSION     DASWZT010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14819
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87070"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VGE92_16780"
     CDS             complement(<1..796)
                     /locus_tag="VGE92_16780"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY1315541.1"
                     /translation="MEGDRIGPYRVLRTLGVGGMGEVFLAERADAEFEQQVAIKVVYG
                     GTRGVRSRLKIERQILAQLDHPNIAHLLDGGSLPDGTAYIVMEYVDGIPIDAYCDSNR
                     LDIEARLRLFQTVCAAVHCAHQNLIVHRDLKPSNILVTAGGAPKLLDFGIAKLLDERQ
                     AGHHTLAVTHADIRIMTPDHASPEQVRGQAITTSSDVYVLGVLLYKLLAGTSPFVIPS
                     MRLSDIERAICEKDPTLPSYSIGKSDAAETRAIADARATSAKRLRRI"
     gene            910..1083
                     /locus_tag="VGE92_16785"
     CDS             910..1083
                     /locus_tag="VGE92_16785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315542.1"
                     /translation="MANGHEPKTPPAGGGGITSPDTGVNKPPQAIVVAAAAAVGGLVG
                     GVVGAMIGSGMHP"
     gene            1085..1987
                     /locus_tag="VGE92_16790"
     CDS             1085..1987
                     /locus_tag="VGE92_16790"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY1315543.1"
                     /translation="MDSLTAYQVLRAPRHETVRLRGLDMHLTRWGPEPSEAASPVFLL
                     HGWLDAGETFQFMVDALRHDRPLVALDWRGFGRSEWPQEGYWFPDYIADLDALLDRLS
                     PNAPARIVGHSMGGNIACSYAGIRPDRVRCVVNLEGFGLARTLPSQAPARMRKWLDQL
                     QGPLKGKDYASLEELAAVIRFRYPRFSAAQAAFVARAWSKVDGSGRVRLAGDPRHHWV
                     NPILYKREDSEACWRELRAPLLMLVGEKSELLTKLAADGTDAAFRAIFPHIEIVRIAD
                     AGHMLHIERPDLVAPLVEAFLDAH"
     gene            2030..2767
                     /locus_tag="VGE92_16795"
     CDS             2030..2767
                     /locus_tag="VGE92_16795"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY1315544.1"
                     /translation="MRTRDGKGVPLVLLHMSPRSGAMWDLLQERLERPTYAPDRLGYG
                     FSDPPPWALTLEQYAQSTVDALKAAGLDGEVDLLGIHTGALEAIEVAHQVSSHVRHVI
                     AVGMPLFSAEEQQRQMEKYSEQPLRPATEGGHVLGAWRGGFAFRQPPYDLSDVHRRFV
                     EHVLAANPGAAFRAACGYPIEKKLKSLKAQLTVFAPHDDLIQQTLRVKPLLKTGAAYL
                     DLPELTHDPFHVAIDRMVTLINRHLPP"
     gene            2784..3422
                     /locus_tag="VGE92_16800"
     CDS             2784..3422
                     /locus_tag="VGE92_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315545.1"
                     /translation="MEIPKVLPAEAPAARAGTATATEPGAQTPPNVAPPTDLLDIRPL
                     DIPAALQILLAEARAALALVLPAAIVPGRANTPAPAMVTASPVQAARALLEMFLQAAP
                     VDAQDPLQWEALLAHVETAFQSSIDAAVNAVAAWRDVPAGVTAAATDTRQLVFSVLAG
                     DSQDPVWLRPEWAGLAPQIQRFWRRRRHMRRRLTDPDYPSSTTDEGEEFAPR"
     gene            3419..3787
                     /locus_tag="VGE92_16805"
     CDS             3419..3787
                     /locus_tag="VGE92_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315546.1"
                     /translation="MRRAGLVFVFMWFLLGGIAHFAFTDLEMRIVPPVIPWPRATVLI
                     TGVFELLGAAGLCLAATRRAAAVGLFALTIAVTPANIYMWQHPELFGAPHWLLVLRLP
                     LQAALLALIVWSTWPGGRRR"
     gene            complement(3807..4169)
                     /locus_tag="VGE92_16810"
     CDS             complement(3807..4169)
                     /locus_tag="VGE92_16810"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEY1315547.1"
                     /translation="MAVSDGYLNYVLEQLSGLGRVTPRRMFGGVGLYHGERFFGVVFR
                     DTLYLKVDDSNRGAYQARGMEPFRPYPDRPHLSMTYYAAPADILEDAEECTVWARKSV
                     AAAGVPKPAPRRRSKRGR"
     gene            4206..5102
                     /locus_tag="VGE92_16815"
     CDS             4206..5102
                     /locus_tag="VGE92_16815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY1315548.1"
                     /translation="MNWKPHLPEIFLGISLAAIAVEAALSRSSVIKAHYKSGDTWASL
                     GMQAGNIVMNLAMAGVVFAGLSAMYKIRLFTISPTSPWAWVLIFFLDDFIYYWFHRIS
                     HECRFWWAAHVNHHSSREYNLSTALRQSWTSVIVGTWTPWIPLALLGFPPSMILTQQG
                     FNLFYQFWIHTEAVHRMPAWFEYVFNTPSHHRVHHATNPVYLDRNYAGVLMLWDRLFG
                     SFAAERDREPPRYGIVKNIETFNPVRIAFHEWAALIREAFAARSAREALGVMFGPPGW
                     RADGQGLTSKNIRSGWESRYGR"
     gene            5092..6225
                     /locus_tag="VGE92_16820"
     CDS             5092..6225
                     /locus_tag="VGE92_16820"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEY1315549.1"
                     /translation="MGVDCKLTGLSGNEIYCMRLKGLIPSGVVIGNSIQSMGFLGGVR
                     SAFRGIVGGEIPDVTGMIHEGREAAFARMRAEADREQVHGVVGVSSELRNLSGNSEFL
                     FVGSGVSGAQGSVRFTSAGDAQELYCHMDAGYEPREFVFGNIAYSVGAVGGIAGTLKT
                     LVRGEIKEFSDVFNETRHHALQRLVTHAKAVRANAVVGVRTNVLHFAGFHEMYMTGTA
                     AFHPQLPSECMSAPVTSDLTGEELWGMAQLGYMPVKLLISTSVYSLGAIGGIKAAFRS
                     FVRGEIGDLTTLIYDAREHVFERLKSEASALGAEEVVGIKTYIIELGSSLIEIFAVGT
                     AIRKQPGMGVNTQLLPAQAIIRDKDTWVSGSGGFDLTSIRAGG"
     gene            6238..6924
                     /locus_tag="VGE92_16825"
     CDS             6238..6924
                     /locus_tag="VGE92_16825"
                     /inference="COORDINATES: protein motif:HMM:NF012659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T2"
                     /protein_id="HEY1315550.1"
                     /translation="MNHARRWLRVSGAAALLACLFFGAIQNGAARHRGRTSGEPGEFA
                     YYLLSLSWSPAFCLSAPGSAECNGPRRYGFIVHGLWPQNERGWPENCDVHRPVPDEVV
                     QSVSDLMPARGLVYHEWSAHGTCSGLEPADFFALVRRARASIAIPLELSRPTQAVEQS
                     PAAIGGAFLRANPRLPPDSVVVTCSAQDVPRLREVHVCLDRELAPRSCAADALRAACR
                     APQVIVPPIR"
     gene            6997..7461
                     /locus_tag="VGE92_16830"
     CDS             6997..7461
                     /locus_tag="VGE92_16830"
                     /inference="COORDINATES: protein motif:HMM:NF018748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1348 family protein"
                     /protein_id="HEY1315551.1"
                     /translation="MDRPPLPPFTAETAAQKARMAEDAWNSRDPQRVALAYTTESRWR
                     NRAEFIQGRDAIQAFLGRKWSRELDYRLIKELWAFRENRIAVRFAYEWHDDSGHWFRS
                     YGNENWEFDEHGLMRERIASINDLPIAERERCYRWPLGRRPDDHPGLSDLGL"
     gene            complement(7532..7669)
                     /locus_tag="VGE92_16835"
     CDS             complement(7532..7669)
                     /locus_tag="VGE92_16835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315552.1"
                     /translation="MTLFMVMVMVMVMGLGLRMMIMVTDMIIMVMRMIARLRSHGRYL
                     T"
     gene            7748..8605
                     /locus_tag="VGE92_16840"
     CDS             7748..8605
                     /locus_tag="VGE92_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY1315553.1"
                     /translation="MAIAVALNIIFVVIEATFGFISNSVALIADAGHNLSDVLGLVCA
                     WTAMYLGRRPPGARFTYGLGRSSVLAALTNAVLLLLACGAIAWEAFGRLASPPAVAGG
                     IVMGVAASGIVINGVSAWMLHAGSHGDLNRRSAFIHMLGDAAVSAGVLLSGALIMYTG
                     WSRLDPIVSLLIVAAILVSTWGLLRDSLHLTLDGVPSSVNSTAVMSYLADQRGVTDVH
                     DLHIWALSTTSVALTAHLVVPDRGAEDSLLDSLTPNLKRRFHIHHATLQIERDRCEHG
                     CENEAQAEA"
     gene            complement(8618..9700)
                     /locus_tag="VGE92_16845"
     CDS             complement(8618..9700)
                     /locus_tag="VGE92_16845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY1315554.1"
                     /translation="MSSITVPVPISWSMRLLERDLLPDFLIRFGIRRLLKARLAEEQQ
                     DSLEAQQRRLTKLIARLRQSPIAVNTADANLQHYELPCAFFERVLGRHLKYSSGYYRN
                     AADSLDEAEANMLELTAERARLTDGERILELGCGWGSLSLWMAEHYPRSQITVVSNSR
                     TQKRFIDARAAQRGFKNLEVITCDVNQLTFPPTTTFDRVVSVEMFEHMRNYAWLMQKI
                     STWLNPAGTLFVHIFAHKTYAYPFEVRDESDWMAKYFFTGGIMPSDDLLLYFQNDLSI
                     QDHWQVDGRHYSQTSDHWLENMDRDRAAIEPILAETYGADQVRRWWVYWRVFFMSCAE
                     LWGYANGQEWLVSHYLFRKSAPALQG"
     gene            complement(9708..10172)
                     /locus_tag="VGE92_16850"
     CDS             complement(9708..10172)
                     /locus_tag="VGE92_16850"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEY1315555.1"
                     /translation="MKSWSHRRILAPLLALLQQGMSPRRLALCVAIGVVVGNVPILGI
                     STLLCTAIALGFRLNLPAIQVVQAAMAPTQILLIIPFVRLGEWILQVPPQPVSIKEGL
                     ALIAQGAGHAIVVLWDAILHAGLAWILVAPGAVYFIYKLLTPVFQRAAAQIK"
     gene            complement(10181..10528)
                     /locus_tag="VGE92_16855"
     CDS             complement(10181..10528)
                     /locus_tag="VGE92_16855"
                     /inference="COORDINATES: protein motif:HMM:NF018991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1475 family protein"
                     /protein_id="HEY1315556.1"
                     /translation="MSTKTALKFLFSCIFLALLVYTAWASTRQSVLQWGGLKSEPDRY
                     WTIATLMDAYFGFLTFYVWVFFKESRCLPRVLWFCAIMLLGNMAMSAYVLLQLTRLRP
                     DQDAAAILTTRNR"
     gene            10650..12290
                     /locus_tag="VGE92_16860"
     CDS             10650..12290
                     /locus_tag="VGE92_16860"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY1315557.1"
                     /translation="MSNESAIRREVGGIALMFTGLGSIIGSGWLFGAWRAAQLAGPGA
                     IYSWIIGAVIILFVALTYAELGAMFPESGGAVRYGHYSHGSLVGFVAGWAAWIAIVSV
                     IPVEAEASVQYMSSWPWAWAQHLYAHAANGQGELTKPGLCISAVLVVIYFLLNFWSVK
                     VFAGTNSAITFFKLIVPAATAITLMCTEFHWENFHIGIHGGRHVGNIASILTAVATSG
                     IVFSYNGFQSPVNLAGEARNPGRSVPFAIFGAIALSTVVYLLLQVAFLGAISPAHLQE
                     GWAALEYSSPFAQLALAMSLNWLAVLLYADAFVSPSGTGTTYTATTARMIYAMERSGT
                     VPAILGRVHPRFGIPRPAMWFNLLVSFIFLFFFRGWGKLAAVISVATIITYLVVPISV
                     MVLRRTAPNVHRPLRVPGLPILAPLAFVLATLMLYWARWPHTGQIMLLLILPMPVYLY
                     YQGKAAWFNFDRQLRAAWWLIAYLLVITGLSWVGSQEFGGLNYIGYGWDQLCVAIAAL
                     IFFSWGLRSGWRTPAIEAVEQRSNSAGASEGQIHTLRP"
     gene            complement(12246..12923)
                     /gene="alkB"
                     /locus_tag="VGE92_16865"
     CDS             complement(12246..12923)
                     /gene="alkB"
                     /locus_tag="VGE92_16865"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: protein motif:HMM:NF011930.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HEY1315558.1"
                     /translation="MSEAAARVRGHTPDLFGAVREVHSERLAAGACLLRGLCLDQATA
                     LLAAIEAVTSAAPFRHWVTPGGFRMSVAMTNCGDLGWISDSSGYRYAACDPQSGRPWP
                     AMPDVFLRLARQAAQAGGFSNFKPTACLVNRYEPGARLTLHQDKDELDLSAPIVSVSL
                     GLPAVFLFGGNARQDRPRRVPVQHGDVVVWGGPARLYYHGVLALKAGNHEILGAQRVN
                     LTFRRAG"
     gene            complement(12916..13233)
                     /locus_tag="VGE92_16870"
     CDS             complement(12916..13233)
                     /locus_tag="VGE92_16870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315559.1"
                     /translation="MRQLSDDPRVLVPRGAQDMMILATWRLTSDSFRPNKRSRMIRIV
                     ISQDALEYYARGNDDARLTSDARFVTWFKQQLSTFDPNHESPLGVEPPAVTWAVSTRI
                     LNE"
     gene            complement(13334..13906)
                     /locus_tag="VGE92_16875"
     CDS             complement(13334..13906)
                     /locus_tag="VGE92_16875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315560.1"
                     /translation="MRRTTAGTSLTPDSAPLDSPQLARRALFGFILTFIAARTVVLLI
                     MSHRMPNLYFFLRGTHVHHLNYGIFLLAGVGAYLLLGARSVRALQVSALVYGAAMALT
                     FDEFGMWLHLGGSYWQRASVDAIIIVAALLGFIAYASAIRSFESRHLRVFIALLLAVT
                     VFGVVLYDTSLRIGRIEGPRLEALEASSSP"
     gene            complement(13937..>14819)
                     /locus_tag="VGE92_16880"
     CDS             complement(13937..>14819)
                     /locus_tag="VGE92_16880"
                     /inference="COORDINATES: protein motif:HMM:NF020071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HEY1315561.1"
                     /translation="FGEPQALSKAEFIVAAELDGAEREARIFLAAPIASTDIDEHFSA
                     HILEHSETVWDDREQAVRARRERRLGAIVLNSTEIRDPEAQALQDAALSGLRKLGIGG
                     LPWTSQLRQWQARVLLMRQHAVPAPEPWPDLSDAALEASLDAWAPPWIAGVTRREHFA
                     RIDLVNALRSRLSYAQAAILEREAPTQFTVPSGSQIPIDYLDGEIPTLSVRLQEMFGL
                     SQTPSVAAGRLPLLLKLLSPARRPVQITRDLVSFWNRGYHEVKKDLKGRYPKHYWPDD
                     PYAAEPTRRARPRLPPR"
BASE COUNT         2742 a         4499 c         4686 g         2892 t
ORIGIN      
        1 ggattcgccg caggcgcttg gcgctggtgg cgcgcgcgtc cgcaatggcg cgcgtttccg
       61 ccgcatcgct cttgccgatc gaatagctgg ggagcgtcgg atccttctcg caaatcgccc
      121 gctcgatgtc gctgagccgc attgacggaa tcacgaacgg gctggttccg gccaacaatt
      181 tgtacaacag aacacccagg acatagacgt cgctcgaggt ggtgattgcc tgtccgcgga
      241 cctgttcggg gctggcatga tcgggggtca tgatgcgaat gtcggcgtgc gtgacagcca
      301 gcgtatggtg gcccgcctgc cgttcgtcca atagcttggc gatgccgaaa tccagcaatt
      361 tgggcgcccc gcccgctgtc accaaaatat tcgacggctt cagatcgcga tgaacgatga
      421 gattctggtg ggcgcaatgc accgcggcgc aaaccgtctg aaacaaccga aggcgcgcct
      481 cgatgtcgag acgattcgaa tcgcagtaag cgtcaatggg aataccatcc acatattcca
      541 tcacgatgta ggccgttccg tccggtaggg aaccgccgtc caacaaatgc gcgatattgg
      601 gatgatcgag ttgcgccagg atttggcgtt cgatcttgag gcgcgaccga acgccgcgcg
      661 tgcccccgta gaccacttta atggccactt gctgctcgaa ctcggcatcc gcccgctcgg
      721 ccaggaaaac ctcacccata ccgccgacac cgagggtgcg cagtacgcga tacggcccga
      781 tgcggtctcc ttccatggcc taaagcatgc gccgcggcgc cgctgaaccg caaacccgcc
      841 ggcgcttgca gcgaagtgcc gcttgcgtgt atgaaagcgg tgcttttcca gaaaatccag
      901 gagtttccca tggcaaacgg acacgaaccc aagacgccgc ctgcgggcgg cggcggaata
      961 acatcccctg acaccggcgt caacaagccg ccgcaggcga tcgtggttgc cgccgccgcc
     1021 gcagtcggcg gtctggtggg tggagtagtc ggcgcgatga tcggcagcgg aatgcatccc
     1081 tagattggat agtctgaccg cgtaccaagt gctgcgtgcg ccgcggcacg agaccgtgcg
     1141 actgcgggga ctcgacatgc atctgacccg atgggggccg gagccttcgg aagccgcgtc
     1201 gcccgtgttt ttgctgcatg gttggctcga tgccggcgag acgtttcagt tcatggtgga
     1261 tgcattgcgg cacgatcggc cgttggtagc gctcgactgg cgcggctttg gacgcagcga
     1321 gtggccgcaa gagggctact ggttccctga ttacattgcc gacctcgacg cgctgttgga
     1381 tcggctgtcg ccaaacgccc cggcgcgcat cgtcggacac agcatgggcg gcaatatcgc
     1441 ctgctcatat gccggcattc ggcccgacag ggtgcgctgc gtcgtcaatt tggaaggctt
     1501 tggactcgcc cgcacgttgc cgtcgcaagc gccggcgcga atgcgcaagt ggttggatca
     1561 actgcaaggt ccccttaagg gaaaggatta cgcttcgctc gaggaactgg ctgcggtcat
     1621 ccgctttcgt tacccgcggt tcagcgcggc gcaggccgcg ttcgtggcgc gcgcctggag
     1681 taaagtcgat ggcagcggcc gtgtgcggct ggcgggcgat ccgcggcatc actgggtgaa
     1741 tccgattctg tataagcgcg aggatagcga agcctgctgg cgtgaattgc gtgcgccgtt
     1801 gttgatgctg gtcggcgaga agtccgagct gctgacaaaa ttagcggctg acggcaccga
     1861 tgcggcattt cgagcgatat ttccgcacat cgagattgtg cgtatcgcgg acgcgggaca
     1921 catgctgcac atcgaaaggc cggacctggt cgcaccgttg gtcgaagcat tcttggacgc
     1981 tcactaaatg ccgcggcgaa tttacccgac tcgctttggg cagatgcata tgcgcacgcg
     2041 tgacgggaaa ggtgtgccgc tggtgttgct gcacatgtcg ccacgctcgg gcgccatgtg
     2101 ggatttattg caggagcggc tggagcggcc tacctacgct ccggaccggc tgggttatgg
     2161 tttttccgat ccaccgccct gggccctgac gctcgagcag tatgctcaga gcaccgtcga
     2221 cgcgctcaaa gccgccgggc tggatggcga ggtcgacctg ttgggcatcc acacgggcgc
     2281 gctcgaggcg atcgaggtgg cacatcaagt cagctcgcat gtacgccatg tcattgcggt
     2341 tgggatgccg ctgttctcag cggaagagca gcagcgccag atggagaaat acagcgaaca
     2401 gcccttgcgg ccggccaccg agggcggcca cgtactcggc gcttggcgcg gcggattcgc
     2461 ttttcgccaa ccgccgtatg atttgagcga tgtgcaccgc cgcttcgtag aacacgtcct
     2521 cgccgcgaat cccggtgcag catttcgcgc ggcgtgcggc tatcccatcg agaaaaagct
     2581 caagagcttg aaagcccagt tgacggtatt cgccccgcac gatgacctca tccagcaaac
     2641 tctgcgagta aagcccttgc tcaaaaccgg cgccgcctat ctcgacttgc cggaactgac
     2701 gcatgaccca ttccatgtcg ccatcgaccg tatggtcacc ctgatcaatc gtcacttgcc
     2761 gccctagggc gcaagattct gcgatggaaa tccccaaagt actgcccgcg gaagcacctg
     2821 ccgcgcgtgc aggaaccgcg acggcgaccg agccaggagc gcaaacaccg cccaacgtcg
     2881 cgccgccgac ggacctcctc gacatccggc cgctcgatat cccggcggcc ttgcagatct
     2941 tgctggcaga ggcgcgcgcg gcgctcgctc ttgtgttgcc ggcggccatc gttcccggcc
     3001 gtgccaacac gccggctcct gccatggtta cggccagccc ggtgcaggcg gcgcgcgcgt
     3061 tgctggagat gtttctgcag gcggcgccgg tggatgcgca ggacccgctg cagtgggagg
     3121 cgctactggc ccatgtcgag accgcgtttc agtcgagcat cgacgctgcg gtcaacgcgg
     3181 ttgcggcgtg gcgcgacgtg cccgcgggag tgacggccgc agccacggat acgcgccaac
     3241 tcgtattctc ggtactcgcg ggcgacagcc aggatcccgt gtggttacgt cccgagtggg
     3301 ccggccttgc gccgcagatt cagcgcttct ggcgccgccg ccgccacatg cgtcgacgtt
     3361 tgaccgaccc ggattatccg tcgtcgacga cggatgaggg cgaggagttc gcaccgcgat
     3421 gaggcgcgcg ggactcgtgt tcgtgttcat gtggttcttg ctcggcggca tcgcgcattt
     3481 tgcgttcacc gacctggaaa tgcgcatagt accgcccgtg atcccctggc cgcgcgcgac
     3541 cgtgttgatt accggtgtat tcgaacttct gggcgcggcc ggcctgtgcc tcgcggcgac
     3601 gcgcagggcc gccgctgtgg gattgttcgc gctcaccatt gcggtcacgc cggccaatat
     3661 ctacatgtgg cagcaccccg aattgttcgg cgccccccat tggttgttgg tgctgcgttt
     3721 gccgctgcag gcggcattgc tcgcgctgat cgtctggagc acatggcctg gcggcaggcg
     3781 gcgctagccg acggcgtttc gccggttcag cggccgcgct tgctacgccg ccgcggcgcc
     3841 ggcttcggca cgcctgcggc cgccaccgac tttcgcgccc agacggtgca ttcctcagcg
     3901 tcctccagga tatcggccgg cgcggcgtaa taggtcatgc tcaaatgcgg tctgtccgga
     3961 tacgggcgga atggttccat gccgcgcgcc tggtaagcgc cgcggttgga gtcatctact
     4021 ttcaagtaca aggtgtcgcg gaaaacgacg ccgaagaacc gctcaccgtg atacagcccg
     4081 acgccgccga acatgcgtcg cggcgtcacg cgccccagac cggacagctg ctcgagcacg
     4141 tagttcaggt aaccatcgct gactgccatg gccccaagaa taagccaagt gatacgatcc
     4201 gcggcatgaa ttggaagcca catctgccgg agattttcct cggcatttcg ctcgcggcga
     4261 tcgcggtcga agcggccttg agccgcagct cggtcatcaa ggctcactac aaatcgggcg
     4321 acacctgggc ctcgctgggc atgcaggccg gcaacatcgt catgaattta gcgatggccg
     4381 gtgtcgtgtt cgccgggctg tctgcgatgt acaagattcg actgttcacg atttcgccga
     4441 catcgccgtg ggcgtgggtg ctcatatttt tcctcgatga tttcatctac tactggtttc
     4501 atcgtatcag ccacgagtgc cgcttctggt gggcagcgca cgtcaatcat cactcgtcgc
     4561 gcgaatacaa cttgtcgacc gccttgcgcc aatcgtggac cagtgtgatc gtcggcacct
     4621 ggacgccctg gattccgttg gcgctgctgg gatttccgcc gagcatgatt ctcacgcaac
     4681 aaggcttcaa tcttttctat cagttttgga ttcatacgga ggcggtgcac cgcatgccgg
     4741 cgtggttcga gtatgtgttc aacacgccat cgcatcatcg cgtgcaccat gccaccaacc
     4801 ctgtttatct ggaccgcaat tacgccgggg tgctgatgct gtgggatcgg ctcttcggat
     4861 ccttcgccgc ggaacgcgat cgagagccgc cgcgttacgg gatcgtcaag aacatcgaga
     4921 cattcaatcc cgtgcgcatc gctttccacg agtgggccgc cctaatccgc gaagcgttcg
     4981 cggcgcgctc ggcgcgcgaa gcactcggtg tgatgttcgg accgccgggt tggcgcgccg
     5041 acggccaagg actcacctcg aaaaacatcc gcagtggatg ggagtctcgt tatgggcgtt
     5101 gactgcaagt tgaccggcct gtccggcaat gaaatctatt gcatgcggct caaggggttg
     5161 atcccaagcg gcgtcgtgat cggcaacagc atccagtcca tgggatttct cggcggcgtg
     5221 cgctccgcat ttcgcggcat cgtcggcgga gagattccgg acgtcaccgg catgattcac
     5281 gagggccgcg aggccgcctt tgcgcgcatg cgagccgagg cggaccgcga acaggttcac
     5341 ggcgtcgtcg gcgtcagctc ggagctgcgt aatctcagcg gcaactcgga gttcttgttt
     5401 gtgggatccg gcgtgagcgg cgcgcagggc agcgtgcgct tcacgagcgc cggcgacgca
     5461 caggaactgt attgccacat ggatgccggc tacgagccga gagaatttgt gttcggcaac
     5521 atcgcctact cggtcggcgc cgtcggcggc atcgcgggca cgctcaagac gctggtgcgc
     5581 ggcgagatca aagagttcag cgacgtattc aacgagacgc gccaccatgc gttgcagcgg
     5641 ctcgtcacgc acgccaaggc ggtgcgagcc aatgccgtcg tcggtgtccg aaccaacgtc
     5701 ctgcatttcg ccggctttca cgagatgtac atgactggaa ccgccgcatt tcatccgcag
     5761 ctgccgtcgg agtgcatgag cgcgccggtg accagcgatc tgaccggcga ggagttgtgg
     5821 ggcatggcac aattgggcta catgcccgtc aagctcttga tctcgacgtc ggtgtattcg
     5881 ctcggcgcaa tcggaggaat caaggcggcc tttcgcagct tcgtgagggg tgagatcggc
     5941 gatcttacga ccctcatata tgatgcacgc gaacatgtat ttgagcggct gaagagcgaa
     6001 gcttcggcac tcggtgcgga ggaggttgtc ggcatcaaga cctacatcat cgagcttggc
     6061 tcgagcttga tcgaaatctt cgcggtcggc accgcaattc gtaagcagcc gggcatgggg
     6121 gtcaataccc agctgctacc ggcgcaagcg atcatccgcg acaaggacac ttgggtgagc
     6181 ggctccggcg gctttgattt aacgtcgatc cgcgccggcg gttgatcggg agattccatg
     6241 aatcatgctc ggcgatggct gcgggtgtcg ggcgcagccg cgttgcttgc ctgcctgttt
     6301 ttcggcgcca tccaaaacgg cgcagcgcgg catcgcggcc gcacttcggg ggagcccggc
     6361 gagttcgcgt actaccttct gtcgctgtcg tggtcgccgg cattttgcct gtccgcgccg
     6421 ggttcggccg aatgcaatgg accgcggcgt tatggtttca tcgtgcatgg actctggccg
     6481 cagaacgaac gaggctggcc cgagaattgc gacgttcatc gcccggtgcc ggatgaggtc
     6541 gtgcagagcg tctcggacct gatgccggcg cgcggcttgg tgtaccacga gtggtcggcg
     6601 cacggcacct gcagcggctt ggaaccggcc gacttctttg cactggtgcg ccgcgcccgc
     6661 gcgagcatcg cgattccact ggaactgtcg cgccctacac aggcggttga gcaatcgccc
     6721 gcggcgatcg gcggcgcatt cctgcgcgcc aatccgcgcc taccgcccga ctcggtcgtg
     6781 gttacctgca gcgctcaaga cgtgccacgg cttcgcgaag ttcacgtctg tctggatcgc
     6841 gagctggcgc cgcgcagctg tgccgccgac gccctgcgcg cggcttgccg cgcccctcag
     6901 gtgatcgttc cgccaattcg ttagacggct cctgagactt gcctcgggac ggctcgcctg
     6961 gttacgctgc tgtgcggccg tcaatgaggt atgcccatgg atagaccccc gctaccaccg
     7021 tttaccgcgg aaaccgcggc gcaaaaggcg cgcatggcgg aagacgcctg gaactcccgt
     7081 gatccgcaac gtgtcgccct tgcctacacg accgagagtc gctggcgtaa tcgcgcggaa
     7141 ttcattcaag ggcgcgacgc aatccaggcg ttcctcgggc gtaaatggtc gcgcgaactc
     7201 gactaccggc tgatcaaaga actctgggcg ttccgcgaaa accgcatagc cgtgcgtttt
     7261 gcctacgaat ggcacgacga tagcggccat tggtttcgca gctacggcaa tgaaaattgg
     7321 gagttcgacg aacacggcct gatgcgggag cgcatcgcga gcatcaatga cctgccgatc
     7381 gccgaacgcg agcgctgtta tcgctggccg ctcgggcggc ggcccgacga tcacccgggt
     7441 ttgagcgatc tggggctcta aaaggccttg ggagcgcttg cgcggccctg tttcgccccc
     7501 atgttgagga acatgctgag atttgggcaa ttcaagtaag atagcggcca tgggaccgca
     7561 gcctagcgat cattcgcatg accatgatga tcatgtcggt gaccatgatc atcatgcgca
     7621 ggcccaaacc catgaccatg accatgacca tgaccatgaa gagggtcatg gcgcggctca
     7681 ggcccgcgcg catgggcatt cccacggaca cgcgcatggc atcgccacgc aaaacatcaa
     7741 tacccgaatg gccattgccg tggccctcaa catcattttc gtggtgatcg aggccacatt
     7801 tggattcatc tccaattcgg tcgcattgat tgccgatgcc ggtcataact tgagcgatgt
     7861 gctcggactg gtttgcgcct ggaccgccat gtatctaggg cgccggcccc cgggcgcccg
     7921 gtttacctac ggattgggcc gctcttcggt gcttgcggcg ctgaccaacg cggtgttgct
     7981 gctgctggcg tgcggagcga ttgcctggga ggcctttggg cgccttgctt cgccgccggc
     8041 cgtggccggc ggaatcgtga tgggggttgc ggcaagcggc atcgtgatca acggtgtgag
     8101 tgcctggatg ctgcatgcag gcagtcatgg cgatctgaac cggcgcagtg ccttcatcca
     8161 catgttgggt gatgccgccg tgtccgccgg cgttcttctg tcgggggcgc tgatcatgta
     8221 tacgggttgg agccggctgg atccgatcgt cagcctgctc attgtcgccg cgatattggt
     8281 gagcacgtgg ggattgctgc gagattcact gcatctaacg ctcgacggcg taccgtcgag
     8341 cgtgaattcc accgcggtca tgtcctattt ggcggatcag cgaggcgtga ccgatgtgca
     8401 cgatctgcac atctgggcgc tcagcacgac cagcgtcgcg ttgacggctc acctagtggt
     8461 gccggatcgc ggcgccgaag attcactgct cgattcgttg acgccgaatc tgaagcgacg
     8521 cttccatatc catcacgcca cactgcaaat cgagcgggat cgctgcgaac acggatgcga
     8581 gaacgaggcg caagccgaag cctaaagcgc gggctggcta gccctgaagg gctggcgcgg
     8641 atttgcggaa taagtaatgt gagaccagcc actcttggcc gttggcgtag ccccacaatt
     8701 ccgcgcacga catgaagaag acgcgccagt aaacccacca gcggcgcacc tggtcggctc
     8761 cgtaggtttc ggcgagaatc ggttcgatcg ccgcgcggtc acgatccatg ttttcgagcc
     8821 agtgatcgct ggtctggctg tagtgacgtc cgtcaacctg ccagtgatcc tgaatgctca
     8881 ggtcattttg aaaatacagc aacaaatcat cgctcggcat gatgccgccg gtaaaaaaat
     8941 acttcgccat ccaatcgctc tcatcccgca cctcgaaggg atacgcatat gtcttgtgcg
     9001 caaagatatg cacgaacagc gtccccgcgg ggttgagcca ggtggagatc ttctgcatca
     9061 accaggcgta attgcgcata tgttcgaaca tctcgaccga caccacgcga tcgaacgtcg
     9121 tcgtcggcgg aaatgtcagt tgattgacat cgcaggtgat cacttcgagg ttcttgaagc
     9181 cgcgttgcgc cgcccgcgca tcgatgaagc gcttttgcgt gcgtgaattc gagaccacgg
     9241 taatctgcga gcgagggtaa tgctccgcca tccacaagga cagcgatccc cagccgcatc
     9301 cgagctccag aattcgctca ccgtccgtaa ggcgtgcgcg ctcggccgtg agctcgagca
     9361 tattcgcttc ggcttcatcc agcgagtcgg ccgcgttacg gtagtaaccc gaggagtact
     9421 tcaagtgccg gccgagtact cgctcgaaga aggcacacgg taattcgtaa tgctgcagat
     9481 tggcatcggc cgtattgacg gcgatgggac tttgccgtaa ccgcgcaatc aacttcgtca
     9541 gacgacgctg ctgcgcttcc aagctgtctt gctgttcctc ggcgagccgc gctttcagca
     9601 agcgtcgaat tccgaagcgg attagaaaat ccggcagtaa atcgcgctcc agcaagcgca
     9661 tcgaccagga tatcggcacg gggacggtta tgctgctcat gggtgcatca cttgatttgc
     9721 gccgcggcgc gttgaaatac cggcgtcaat aacttataga tgaaatagac cgcacccggc
     9781 gccaccagga tccaggccaa tcccgcatgc aggatcgcat cccagagcac gacgatggcg
     9841 tgtcccgccc cttgcgcaat gagagcgaga ccttctttga tcgatacggg ctgcggagga
     9901 acttggagga tccactcacc gaggcgcacg aacgggatga tcagcaaaat ctgggtgggc
     9961 gccatggcgg cctgcacgac ttggattgcc ggcagattca agcgaaatcc gagtgcgatt
    10021 gcggtgcaaa gcagggttga gattccgagt atgggaacgt tgccgaccac caccccgatc
    10081 gccacgcaca gcgctaagcg cctgggtgac atgccttgct gcaacaaggc gagcagcggc
    10141 gcgagaatcc tgcgatgcga ccagctcttc acgggcgcag tcagcgattg cgcgtcgtga
    10201 gaatcgccgc tgcgtcttga tcggggcgca gccgcgtgag ctgcagcagc acataggcgg
    10261 acattgccat attgccgagg agcataatgg cgcaaaacca caagacgcgc ggcaagcaac
    10321 gagattcctt gaaaaatacc cacacgtaga aggtcaaaaa gccgaaataa gcgtccatca
    10381 gggtggcgat cgtccaataa cggtccggtt cggatttcag gcctccccac tgcaacacgg
    10441 attgccgagt gctggcccat gccgtgtaca ccagcagcgc cagaaaaatg caggaaaaca
    10501 ggaattttaa ggccgttttg gtcgacatgg gcgtacatct cacgggttat tcggctgcgc
    10561 gggccggtcg gattcaggat tttgcaagat atcggaatgg cttaaggccg gtcgagtcgc
    10621 tagagtcgat tgtccaggga gaaccaagta tgtccaatga aagtgcaatt cgacgcgagg
    10681 tcggtggcat cgcgctgatg ttcaccggct taggctcgat catcggctcg ggttggttat
    10741 tcggggcctg gcgcgccgcg caacttgccg gtccgggagc gatctattcc tggatcatcg
    10801 gcgcggtgat catcctgttc gtcgccctga cctatgcaga actcggggcg atgtttccgg
    10861 agtcgggcgg cgccgtgcgc tatgggcact attcacacgg ctcgctggtc ggattcgtcg
    10921 cgggctgggc cgcctggatt gccatcgttt cggtgattcc ggtggaggcc gaggcctcgg
    10981 tgcaatacat gagttcctgg ccctgggcct gggcacaaca cttgtacgct catgccgcca
    11041 atggtcaggg tgagttgacc aagccaggcc tgtgcatttc cgcggtgctg gtggtgatct
    11101 atttcctgct gaatttttgg agcgtcaaag tttttgccgg taccaacagt gcgattacat
    11161 ttttcaagct gatcgttccc gcggcgaccg ccatcacgct gatgtgcacc gaatttcatt
    11221 gggaaaattt tcacatcggt attcacggcg ggcgccatgt gggcaacatc gcctcgatcc
    11281 tgaccgcggt cgccaccagc ggcatcgtat tcagttacaa cggattccaa agcccggtca
    11341 atctcgcggg ggaggcccgc aatccgggcc gcagcgtgcc gtttgcgatc tttggagcga
    11401 ttgccctcag caccgtggtg tacctgctgt tgcaagtcgc cttcctggga gcgatttctc
    11461 cagcgcatct gcaggaaggc tgggccgccc tggagtacag ctcgccgttt gcgcagctgg
    11521 cgctcgcgat gagcctgaac tggctcgccg ttctgctgta cgcagatgcc tttgtcagtc
    11581 caagcggcac cggcacgacg tatacggcga ccacggctcg aatgatttat gccatggagc
    11641 gcagcggcac ggtgcccgcg atactcggcc gcgtgcatcc gcgcttcggg atcccacggc
    11701 cggccatgtg gttcaatttg ttggtgtcgt tcatttttct cttcttcttc cggggttggg
    11761 gcaagctcgc ggcggtgatc tcggtggcca cgatcattac gtacctggtg gtaccgatca
    11821 gcgtcatggt gctgcggcgc acggcgccga atgtgcaccg gccactgcgc gtgccgggcc
    11881 tgcccatcct cgcgccgctc gcctttgtgc tggccaccct gatgctgtac tgggcgcgct
    11941 ggccgcacac gggccagatc atgctgctgt tgattctgcc gatgccggtg tatctgtact
    12001 accaaggcaa ggccgcttgg ttcaatttcg atcggcagct gcgcgccgct tggtggctga
    12061 ttgcctactt gctggtgatc acgggtttgt catgggtcgg cagccaggaa tttggcgggc
    12121 tcaactacat cggctacgga tgggaccagc tgtgcgtggc gatcgccgcg ttgatctttt
    12181 tttcctgggg acttcgcagc ggctggcgca cgcctgcgat cgaggcggtg gagcagcgtt
    12241 cgaattcagc cggcgcgtcg gaaggtcaga ttcacacgct gcgcccctag gatttcgtgg
    12301 ttacccgctt tcagcgcgag cacaccgtgg tagtacagac gtgcgggacc cccccaaacc
    12361 acgacatcgc cgtgctgcac cggcacgcgg cgcggtcggt cctgtcgagc gttgccgccg
    12421 aacagaaaaa ccgcgggtag accgagcgat accgacacaa tcggtgcact caggtcgagt
    12481 tcgtccttgt cctggtgaag ggtcagccgc gcacccggtt cataacggtt gacgaggcac
    12541 gcggttggct tgaagttcga gaacccgccc gcctgcgctg cctgccgggc cagcctgaga
    12601 aagacgtcgg gcatcgcggg ccacggccgc ccgctctgtg gatcgcaggc agcatagcga
    12661 taaccgctgc tgtcgctgat ccacccgagg tcgccgcaat ttgtcatggc gaccgacatt
    12721 cgaaatccgc ccggtgtcac ccagtggcgg aacggcgcgg cggacgtgac ggcttcgatg
    12781 gccgccaaga gggccgtcgc ctgatccaaa cagagtccac gcagcaagca cgcgcccgcc
    12841 gcaagacgtt cgctgtgcac ctcacgcacg gcgccgaaca aatccggcgt gtggccgcgc
    12901 actcgtgccg ccgcttcact cattcaatat gcgggtgctc accgcccagg tgaccgccgg
    12961 cggctcgact ccgagcggcg attcatgatt gggatcgaag gtgctcaatt gctgtttgaa
    13021 ccaagtgacg aagcgcgcgt cgctggtgag acgcgcatca tcgttgccgc gagcgtagta
    13081 ctcgagcgcg tcctgcgaaa tcacgatgcg gatcattcgc gagcgtttgt tcgggcggaa
    13141 cgaatcgctg gtgaggcgcc acgtggccag gatcatcatg tcttgcgcgc cgcgcggcac
    13201 cagcactcgt gggtcgtcgc tcagctgacg catgacgagc tctcgaaaat gattactgaa
    13261 gtctttcatc gcgtaccatt gtaaccaaat cgccgaacgc cgcgtcgcga agcggagata
    13321 ttccgggcgg cgatcaaggg gaagaagagg cttcgagggc ctcgaggcgt ggaccttcga
    13381 tgcggccgat ccgcagcgac gtatcgtaga gcacgacgcc aaacacggtc acggccagca
    13441 gcaaggcgat gaaaaccctg aggtggcggg actcgaagct gcggattgcc gacgcatacg
    13501 caatgaagcc gagcagggcg gcaacgatga tgatggcgtc gacggaggcg cgttgccaat
    13561 agctgccgcc caaatgcagc cacattccga attcatcgaa cgtcaacgcc atcgcggcgc
    13621 catagaccag ggcggagact tgcagcgccc ggacgctgcg tgcgcccagt agtaaatagg
    13681 cacccacccc tgccagcagg aaaattccgt agttaaggtg atggacgtgc gtgccgcgca
    13741 gaaagaaata caggttcggc atccgatgcg acatgatcag gagcacaacc gtgcgtgccg
    13801 cgatgaaggt cagaatgaaa ccgaacagcg cgcggcgcgc gagctggggt gagtcgaggg
    13861 gtgcggaatc aggcgtcaat gaagttccgg cggtcgttcg ccgcataccc taactgctgc
    13921 cgggcggttg tcaatactag cgcggcggca aacgcggccg cgcgcggcgc gtcggctcag
    13981 ccgcgtatgg atcgtcgggc caataatgct tcgggtatcg acctttgagg tccttcttca
    14041 cttcatggta gccacggttc cagaagctga cgagatcgcg cgtgatctgc accgggcgcc
    14101 tcgcgggcga cagcaatttc agcagcaagg gcaggcggcc ggcggcgacg ctcggcgttt
    14161 ggctcaatcc gaacatttcc tgcaagcgta cggacaacgt cgggatctcg ccatccaagt
    14221 aatcgatcgg aatttgcgag ccgctgggca cggtgaactg cgtcggcgct tcgcgctcga
    14281 gaatggcggc ttgcgcgtag ctcaaacgcg atcgcagggc attgaccaga tcgatccgtg
    14341 caaaatgctc acgtcgagtc acgcccgcaa tccagggcgg tgcccaagca tcgaggctcg
    14401 cctcgagcgc cgcatcgctc aggtccggcc acggctcggg cgcgggaacc gcgtgttgtc
    14461 tcatcaacag gactcgcgcc tgccactggc gcaactgcga ggtccacggc agcccgccga
    14521 ttcccagctt ccgaagtcct gaaagcgccg catcttgcag cgcctgggct tcagggtcgc
    14581 ggatctcggt cgaattcagc acgatggcgc cgagacgccg ctcgcgccgc gcgcgaaccg
    14641 cctgttcgcg gtcgtcccag acagtttcgg agtgctcgag aatgtgcgcc gagaaatgct
    14701 catcgatatc ggtggatgct atcggagctg cgagaaagat ccgcgcctcg cgctccgcgc
    14761 cgtccagctc ggcggcgacg atgaactctg ctttggagag cgcttgcggc tcgccgaac
//
LOCUS       DASWZT010000587         6207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_77374, whole genome shotgun sequence.
ACCESSION   DASWZT010000587 DASWZT010000000
VERSION     DASWZT010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6207
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77374"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /locus_tag="VGE92_16885"
     CDS             <1..1222
                     /locus_tag="VGE92_16885"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY1315562.1"
                     /translation="NLDLAAVAFARLKDLEQRGLPDAADLAASFADLYADHGMASAVE
                     IMTIHKAKGLEFDLVVVPALDRHIAQSRDQLLMSHQFARTGRDGLVMAARPAVGADKD
                     RLFDFLRHQVRDGAGLEAQRLLYVACTRAKWQLHLTATIDRQEQPAETAEFRPARVWS
                     PRAGSLLAVLWPVVGEEFAVANADSAMRGRAAGAPRGGLLARVPKDWAPNFDAAPMLD
                     ALPTSPSMREETPVFDWAGETARRVGTLVHAELQALDLARVDASKIRGREPHFRHWLA
                     SLGVPAERLGQAAARVVEALVAVIDDPRGRWILKASREESREQAISGRLQGEVVRAVF
                     DRSFIDEQGVRWVIDYKTSQHSGGGLDEFIDREVERYRPQLQRYARLARRLGPEPVRL
                     GLYFPLMRAWREWD"
     gene            1280..1648
                     /locus_tag="VGE92_16890"
     CDS             1280..1648
                     /locus_tag="VGE92_16890"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY1315563.1"
                     /translation="MIGYVTIGTNDLTRAAAFYDALLAEVGAKRMMENEQFIAWSARP
                     EAPGLGVTKPFDKKPATVGNGVMVALEASSPAQVDALHRKALQLGATDEGAPGPRGGG
                     FYAGYFRDLDGNKLNFFHMG"
     gene            complement(1662..2471)
                     /gene="gspN"
                     /locus_tag="VGE92_16895"
     CDS             complement(1662..2471)
                     /gene="gspN"
                     /locus_tag="VGE92_16895"
                     /inference="COORDINATES: protein motif:HMM:NF013376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HEY1315564.1"
                     /translation="MPVSRSPNRDTKPVPRSRSIWPLLLIGLAAVLAVIVAVLPASAV
                     AHFLPASVQAEDFSGSIWHGSAGKFTVNAHNAGALEWRVHPGALLGLALAVDVHWVKT
                     GFVVDARVSFDRQGFAAHAVKGGGPIEDLQEFGVAVGWSGIAGIDFSEIKGHFAQYLS
                     AVGDVQVSNLTAPQIAEGADLGGYDLHVGAGAVASDGSVTAQLKDTGGPLDLQAVVRY
                     SVKQHLGLLTGTVKERANAPPALLQQLQSLSQLRGRDPEGRIPIDLEFTAP"
     gene            complement(2437..2922)
                     /locus_tag="VGE92_16900"
     CDS             complement(2437..2922)
                     /locus_tag="VGE92_16900"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein M"
                     /protein_id="HEY1315565.1"
                     /translation="MNKLRAWYDGLQQREQRMVSFGGVVVAVLLLLFGLLMPLQSAVS
                     NAVARNAAKREDLAWMQVNATEVRAAGGAVATDTGEAPVVLVDRVGRESGLGNALRGT
                     QPNGTGVRVQLEAAPFDTLVAWLATLDEHYGLAVESITVDRAARPGIVNASITFSQPR
                     H"
     gene            complement(2919..4184)
                     /gene="gspL"
                     /locus_tag="VGE92_16905"
     CDS             complement(2919..4184)
                     /gene="gspL"
                     /locus_tag="VGE92_16905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HEY1315566.1"
                     /translation="MPQTLLLRLPSPGQEETEWLTIEDSGESETTRQRGSLTLAAAVS
                     RSGKVVALAPATQILLAEPELPPGSGVKLARAVPFALEEQLTEDVDQLSFAVGRRRPS
                     GTTPVAAVSRAVLHGWVEDLHAAGLEPLAIYPDISLMPDNPSLTVLWLEGERLSVRRP
                     RTLPFAVELSPVSEALVVAGVIPDPLDTTSEPKAAESAVLYITREDWTRVQDEFEDLS
                     AKFESLKVQLLPDGPLPWLARGLADTEAVNLLQGEFARATDYGARWRQWRTAAALALG
                     LLVVHVAAQAVQIRQAKHETAVLDGQIASVFSSAMPNETLQDPRRQMQSRLDRIRKSG
                     AGPEEFLRTLKVLGGAMAVTPKTDIQALSFREDSLDMKVNTQSLASLSQLSQFIGTQG
                     MSAEIQSSTPVAGGVEAHIQVRTPARAHR"
     gene            complement(4191..5210)
                     /gene="gspK"
                     /locus_tag="VGE92_16910"
     CDS             complement(4191..5210)
                     /gene="gspK"
                     /locus_tag="VGE92_16910"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HEY1315567.1"
                     /translation="MSPVRPSGACRTRRAPRARQRGVALIIALILVALATVLATKLTF
                     DGFLERRRTIGVLAAEQAVHFGMGAEALAADVLAQDAQTSASYTLLTGAWAQPTQPLP
                     ITPQDDPEGEPIGTVQGELEDMQGRFNLNNLARLVYGPDGKTFVEDPLPLEQFTRLLV
                     SVGLEAKWAGMARDWIDPDDVPSSPDGAEDQIYTSQTPPYRTGNWPMMSPAELMNLPG
                     FGADRFRKIAPYVTALPSATVPINICTASAPVLMSLADNLNGEYSPEVLANGRKSGCF
                     PDRAAFTNILGGKGPPATAWSTYSQYFRLTSRVTLGTTEFTLYSLLKRGNGKVTPLLR
                     SFGTS"
     gene            complement(5207..5959)
                     /gene="gspJ"
                     /locus_tag="VGE92_16915"
     CDS             complement(5207..5959)
                     /gene="gspJ"
                     /locus_tag="VGE92_16915"
                     /inference="COORDINATES: protein motif:HMM:NF023044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HEY1315568.1"
                     /translation="MKHRTQHGELRSRGFTLIEIMVALLILGIMSALGYGTYRAARIS
                     AERTEAALKRSREIEFGMRIMVQDIAETVPRPVRDILGQTRLPAMQGTGGIGTLTALP
                     SSSVGASTGPGMTFASQSFNSSPNFGGSTASSVASLIDLTRAGWSNTAGQQRGTLQRV
                     SYGLVDDVLKRSYQVNLDTVQGDKPVVQDLLTGIKAIQLRYLDNNQTWQTQWPSPALP
                     PSDGLWSRPAAVELIIEFKDWGRIRRLIEVAG"
     gene            5975..>6207
                     /locus_tag="VGE92_16920"
     CDS             5975..>6207
                     /locus_tag="VGE92_16920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1315569.1"
                     /translation="MGVGAGACGVVVVWPLVVGVAAPLTDTPVVVAALDALATADACV
                     EPVVQSAVLEFPAALKALPKNPVDSASGLSFVLA"
BASE COUNT         1092 a         2005 c         2033 g         1077 t
ORIGIN      
        1 aaacctcgac ctcgccgcag tcgcattcgc gcgcctcaag gatttggagc agcgcgggtt
       61 gccggatgct gcggacctgg ccgccagttt cgccgatctg tatgccgacc acggcatggc
      121 gtccgcggtc gaaatcatga ccatccacaa agccaagggg cttgagttcg acctggtcgt
      181 ggtgccggcg ctcgatcggc acatcgcgca gagccgcgac caattgttga tgtcccatca
      241 attcgcccgt accggccgcg atggcctggt gatggcggcg cgtccggcgg tcggcgccga
      301 caaggatcga ctgttcgact tcctgcgcca ccaagtgcgc gatggcgcag gactcgaagc
      361 gcagcggctg ctgtacgtcg catgcactcg tgcaaaatgg cagctgcatc tgaccgcgac
      421 gattgaccgg caagagcagc cggccgagac ggccgaattt cgacctgccc gcgtctggtc
      481 gccgcgggcg gggagtctac tggccgtgct ctggccggtg gtgggcgagg aattcgccgt
      541 cgcgaatgcg gattccgcga tgcgcgggcg cgcggcgggc gcgccgcgcg gcgggctgtt
      601 ggcgcgcgtg ccgaaggact gggcgccaaa cttcgatgct gcgccaatgc tggacgcgct
      661 gccgacctcg ccgtcgatgc gtgaggaaac cccggtcttc gattgggccg gcgagacggc
      721 gcgtcgcgtc ggtaccttgg tgcacgccga acttcaggcg ctcgatcttg cgcgcgtcga
      781 cgcaagcaaa atccgcggac gcgagccgca ttttcggcac tggctggcct cgctcggcgt
      841 gccggcggag cggctcggcc aagccgccgc gagggtcgtc gaggccctgg tcgccgtgat
      901 tgacgacccg cgcgggcgct ggattctcaa ggcgagccgc gaggagtcac gcgagcaggc
      961 gatatcgggt cggctgcaag gggaggtggt gcgggccgtg ttcgatcgca gcttcatcga
     1021 tgagcagggc gtgcgttggg tgatcgacta caagacgagc caacacagcg gcggcggtct
     1081 cgatgaattc atcgaccgcg aagtggaacg ctaccggccg caattgcaac gctatgcacg
     1141 actcgcccgc aggctcggtc cagaaccggt ccgcctcggg ctgtatttcc cgctgatgcg
     1201 ggcatggcgc gaatgggatt gacgcgaggg gttactggat atataaccag cttcggccaa
     1261 aaacaaccgg agcaaaccca tgatcggata cgtaaccatc gggaccaatg acctgacgcg
     1321 cgccgctgca ttttacgacg ctctgctggc ggaggtgggc gccaagcgga tgatggaaaa
     1381 cgagcaattc atcgcgtggt cggcgcggcc cgaggcgccc ggcctcggtg tcaccaaacc
     1441 gttcgataaa aagccggcaa cggtgggcaa cggggtcatg gtggcgctcg aggcaagcag
     1501 cccggcgcaa gtcgatgcct tgcatcgcaa agccctgcag ttgggcgcca cggacgaagg
     1561 cgcgcccggg ccgagaggcg gcggattcta tgcgggctat tttcgcgatc tggatggtaa
     1621 caagctcaac ttcttccaca tggggtagaa gcttgccgcc cctagggcgc ggtgaattcc
     1681 aggtcgatgg ggatgcgacc ctcagggtcg cgcccgcgca gctgcgatag gctttgcaac
     1741 tgctgaagca gcgccggggg ggcgtttgcg cgctccttca ccgtgcccgt caaaagcccc
     1801 aggtgctgct tcacggagta gcggacgacg gcctgcaaat cgaggggccc ccccgtgtct
     1861 ttgagctgcg cggtgacgga accgtcgctg gcgactgcgc cggcgcccac gtgtagatcg
     1921 taacccccta aatccgcgcc ctccgcaatt tgcggagcgg tcagattcga tacctgcaca
     1981 tcgccgacgg cgctcaaata ctgcgcgaag tgtcctttga tctcgctgaa gtcgattccg
     2041 gcgatgccgc tccagccgac ggccactccg aattcctgca ggtcttcgat cgggccgccg
     2101 cccttgacgg catgcgcggc gaatccctga cgatcaaagc tgacacgagc atcgacgacg
     2161 aaccccgtct tgacccaatg cacgtcgaca gccagggcga gcccgagcag tgcgcccgga
     2221 tgcacgcgcc actcgagcgc gccggcgttg tgcgcattca cggtgaactt tcctgccgag
     2281 ccgtgccaga tgctgcccga gaaatcctcc gcctgcaccg aagcgggtaa aaaatgcgcc
     2341 acagccgacg cgggtaacac ggcaacgatc accgccagca ccgccgcgag gccgatgagc
     2401 agcaacggcc aaatcgagcg cgagcgcggg accggcttag tgtcgcggtt gggagaacgt
     2461 gatactggca ttcacgatcc ccggccgggc ggcgcgatcg acggtgatcg attcgaccgc
     2521 cagcccgtaa tgctcatcga gcgtcgcgag ccaagcgacc aacgtatcga acggcgcggc
     2581 ctccagctgc acgcgcacac cggtgccgtt cggttgtgtg ccgcgcagcg cgttgccaag
     2641 gcccgactcg cgcccgactc ggtccaccag caccaccggc gcttctcccg tgtcggtggc
     2701 caccgcgccg ccggcggcgc gaacttccgt ggcattcacc tgcatccagg ccagatcttc
     2761 gcgcttagcg gcattgcgcg cgaccgcatt cgatacggcg gattgcagcg gcatgagcag
     2821 tccgaacagc agcagcagca ccgccaccac gactccgcca aagctcacca tgcgctgttc
     2881 gcgttgttgc aggccgtcgt accacgcacg cagtttgttc atcggtgcgc ccgcgccggg
     2941 gtacgaacct gaatgtgcgc ctcgacaccg ccggcgacgg gcgttgagga ctgaatctcc
     3001 gcgctcatgc cctgcgtgcc gataaattgc gacagctgcg acagcgacgc gaggctctgg
     3061 gtattgactt tcatatcgag cgagtcctcg cgaaaactca acgcctggat atcggttttc
     3121 ggcgtcacgg ccatggcacc gcccagtacc ttcaaggtgc gcagaaactc ttccgggccg
     3181 gcgcctgatt tgcggatgcg atcgaggcgc gactgcatct gacggcgcgg atcctgcaaa
     3241 gtctcgttgg gcatggccga ggagaaaacg ctggcgattt ggccgtcgag gaccgcggtc
     3301 tcatgcttgg cctggcggat ttgcaccgcc tgtgcggcca cgtgcacgac cagcagaccc
     3361 agcgccaatg cggcggcggt gcgccactgg cgccaacgcg cgccgtaatc ggtggcacgc
     3421 gcaaactcgc cttgcagcag gttgacggct tcggtgtccg ccagtccgcg ggccagccag
     3481 ggcagcggcc catcgggcaa tagttgtact ttcagcgatt cgaatttcgc cgacagatct
     3541 tcgaattcat cttggacgcg ggtccaatct tcgcgcgtga tgtacagcac ggcgctctct
     3601 gcggccttgg gttccgaggt cgtatccaga ggatccggta tgacgcctgc gaccaccaag
     3661 gcttcgctca ccggagagag ctccaccgca aacggcaatg tcctcggacg tctcaccgag
     3721 agccgttcgc cttccagcca aagcacggtg agagacgggt tgtccggcat caatgagatg
     3781 tcgggataaa tcgccaaggg ttcgagcccg gcggcgtgca agtcctcgac ccagccgtgc
     3841 aggacggcgc gcgacacggc ggccaccggc gtggtgccgc tcggccggcg tcggccgacg
     3901 gcaaagctca gctgatcgac gtcctcggtc agttgttctt cgagggcgaa cggcaccgcc
     3961 cgagccagct tgacgccgct gccgggcggc aactcgggtt ccgccaatag aatctgcgtc
     4021 gccggggcga gtgccacgac ttttccggac cgcgacaccg ccgcggccaa ggtcagcgac
     4081 ccgcgttgcc gggtggtctc cgattcgccg gagtcctcga tggtcagcca ctcggtctct
     4141 tcttggccgg gcgagggcag acgcagcagt aaagtttgtg gcatagatcc ttagctagtt
     4201 ccaaaggaac gcagcaaagg tgtgactttg ccgttcccac gcttcaaaag actgtacaag
     4261 gtgaactccg tagtgccaag cgtcacgcgc gaggtcaggc ggaaatactg gctgtaggta
     4321 ctccatgccg tggccggcgg tcccttgccc ccgaggatgt tggtaaaggc ggcgcgatcg
     4381 ggaaaacaac cgctcttgcg gccgttggcg aggacctccg ggctgtattc tccattcaaa
     4441 ttatcggcca gactcatcag caccggcgcg gacgccgtgc agatgttgat cggcacggtc
     4501 gcggatggca gtgccgtgac atagggcgcg atcttgcgga agcggtcggc gccgaacccg
     4561 ggcagattca tcagctccgc cggcgacatc atcggccagt tgccggtgcg atagggcggc
     4621 gtctgcgagg tatagatctg atcctcggcg ccatccgggc tgcttggaac atcatcggga
     4681 tcgatccaat cgcgcgccat accggcccat tttgcctcga gcccgaccga caccagcaag
     4741 cgcgtgaact gctcgagcgg cagcggatcc tcgacgaagg tcttgccatc cggaccgtac
     4801 accaggcggg ccaagttgtt gaggttgaag cgtccctgca tgtcctccag ttccccttgc
     4861 acggtgccga tgggttcgcc ttcgggatca tcctgcggcg tgatcggcag cggttgcgtg
     4921 ggctgcgccc aagccccagt caacaaggtg tagctggcgc tggtctgggc atcctgcgcc
     4981 agcacatcgg ccgcgagcgc ctcggcgccc atgccgaaat gcacagcctg ttctgcggca
     5041 agcacaccga tggtgcgtcg gcgctcgaga aacccatcga aggtcaattt cgtcgcgagc
     5101 acggtggcca aggccaccaa aatcagggcg atgatgagcg ccacaccgcg ttgccgtgcg
     5161 cgcggcgccc gccgcgtgcg gcacgctccg ctgggtcgca ccgggctcat ccggccacct
     5221 cgatcagacg gcggattctt ccccaatcct tgaattcgat gatgagctca accgccgccg
     5281 gccgactcca aaggccatcg gagggcggta gggccgggga gggccattgc gtctgccagg
     5341 tctgattgtt atcgaggtaa cgcagctgaa tggctttgat ccccgtgagc aagtcctgca
     5401 ccaccggctt gtctccctgc acggtgtcga ggttcacctg gtagctgcgc ttcagcacat
     5461 catccaccag tccataggaa acgcgctgca aagtgccgcg ctgctgaccc gccgtgttcg
     5521 accagccggc gcgcgtcaaa tcgatgagcg aggcgacgct tgaagcggtg ctgccgccga
     5581 agttcggact cgagttgaag ctctggctcg caaacgtcat tccggggccc gtgcttgcgc
     5641 cgacgctgga actgggcagc gcggtgaggg tgccgatacc gcccgtgccc tgcatcgccg
     5701 gcaagcgcgt ttggcccaaa atatcgcgaa ccggacgcgg caccgtttcc gcgatgtcct
     5761 gcaccatgat gcgcattccg aactcgattt cgcgggagcg cttgagcgcg gcctcggtgc
     5821 gctcggccga gatgcgcgcc gcgcggtacg tgccatagcc cagagccgac atgatcccca
     5881 ggatcaacag cgccaccatg atttcgatca aggtgaaacc gcggctgcgc aactcgccgt
     5941 gctgtgtccg atgcttcatt gggcaggtgt cgttgtggga gtgggcgctg gagcctgcgg
     6001 ggtggtcgtc gtctggccgt tggtggtggg tgtcgccgcg ccgctaaccg acaccccggt
     6061 cgtggtcgct gcgctcgacg cattggccac cgcggacgcc tgcgtcgagc cggtcgtcca
     6121 gtcggccgtg ttggaattcc ccgcggcgct caaggcgctg cccaaaaatc cagtcgactc
     6181 ggccagcgga ttgtctttcg tcttggc
//
LOCUS       DASWZT010000588         6758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U3745
            k141_19319, whole genome shotgun sequence.
ACCESSION   DASWZT010000588 DASWZT010000000
VERSION     DASWZT010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959552
            Sequence Read Archive: SRR1157616
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 05:03:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,392
            CDSs (total)                      :: 3,369
            Genes (coding)                    :: 3,355
            CDSs (with protein)               :: 3,355
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19319"
                     /isolate="SMAG_U3745"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="47.50505 N 93.48901667 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VGE92_16925"
     CDS             <1..667
                     /locus_tag="VGE92_16925"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1315570.1"
                     /translation="IRGGSREYVKQLTAPYAKGLLLNTPVASLQRLPTRIVVRLKNGS
                     VENFDQVFIACHSDQALKLLSDASHQEREILGAIPYQANEALLHTDSSLMPRRSLAWA
                     AWNYHLPIEQFGRVTVTYNMNILQSLDAPAQFLLSLNRGDAVDPNKVLGRYVYHHPVY
                     TAAAVAAQGRRHEINGVRRTYFCGAYWGYGFHEDGVNSALASLEEFQRRQAHEQPNLQ
                     RVG"
     gene            636..1388
                     /locus_tag="VGE92_16930"
     CDS             636..1388
                     /locus_tag="VGE92_16930"
                     /inference="COORDINATES: protein motif:HMM:NF018770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HEY1315571.1"
                     /translation="MNSRIYKGWVEHRRFAPRCNRFRYPLFMLYLDLAELPRLFARTP
                     LWSATRPALAWFKREDYLAPHNLPLEQAVRDLVAARTGRRPSGPIRLLTHLRYFGYSM
                     NPVSLYYCFDESGGTLETIVAEITNTPWRERHQYVLNVDSAASRLKRFDFDKGFHVSP
                     FMPMDMRYRWSFSEPGQRLFVNMRNFQGAAHVFDATLSLRGEPVTRGALLGALFSYPL
                     LSLRVITAIHWQALRLWLKRTPFYGHPRTLGR"
     gene            1398..2684
                     /locus_tag="VGE92_16935"
     CDS             1398..2684
                     /locus_tag="VGE92_16935"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEY1315572.1"
                     /translation="MKHSIIPLEIAAKPERGSAFSKLAKGLLLSQLRKIRDGRLRLID
                     ADHAETFGEITAAGPFDVTIRVRHPRFYTDVVLAGTLGSGEAYMNGYWQCDDLTALVR
                     LMVINRHLMNDVDSGWSRLSAPLLRFAHWLNRNDKSGSRRNIAAHYDLGNHFFSLFLD
                     ETMAYSSAIFARADATLLEASTAKFDAACSKLDLKPDQHLLEIGTGWGGLAIHAAKNY
                     GCRVTTTTISREQFEFAKESVARAGLSERITLLLEDYRDLKGQYDALVSIEMIEAVGD
                     RYLDTYFRRCSGLLRASGAMLLQSITIRDQFYAQARRSVDFIKRFIFPGSCIPSIQVL
                     VASVARATDLKLFHMEDIGPHYARTLQAWRERFLARQAEVRALGYPESFVRMWEFYLC
                     YCEGGFEERQLGDVQMLLTKPRSRRAAIACVNAAGA"
     gene            2668..4098
                     /locus_tag="VGE92_16940"
     CDS             2668..4098
                     /locus_tag="VGE92_16940"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY1315573.1"
                     /translation="MPRAPDARTLDELKAVVGAGFWLEAAEAVAPFVNDFRRLYRGAT
                     PLVLAPHDTGEVAQILRICNRESVAVVPHGGNTGYCGGATPDASGTEIVLSLRRLKRV
                     RQIDAVNYSMVIEAGCTLAEAQAAAADVDRLFPLSLGSEGTAQIGGNLSTNAGGTAVL
                     RYGMMRDLVLGLEVVLADGSVLEGLKSLRKDNTGYDVKSLFVGAEGTLGVITAACLKL
                     FPRPADTATALVGIDSPPRALDLLAGLRTAGGDQVTSFELIPHRAVELTVKHVPGVAD
                     PSSQNAPWYLLVELSSPNPRHELSALLTAALEDAVLRGTIRDAMLATSLAQAQAMWKL
                     RESVPEAQRRHGASLKHDVSVPVSAIPALIEEGSLLVRRLAPEGEVIAYGHAGDGNLH
                     FNVSQRPECDVQSFLARGPALELAIFDLVERLGGSISAEHGIGRLKAAEFARRADPVE
                     LAVMHAMKHALDPNGIMNPGKVLCAR"
     gene            4086..4646
                     /locus_tag="VGE92_16945"
     CDS             4086..4646
                     /locus_tag="VGE92_16945"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY1315574.1"
                     /translation="MRTLILPVCTARLQLREFVRSDFDAVFAYSSDPRVTRYLFFGPR
                     DADGTSDYLDGLLASQHERPRTRFELAVEETASGRVIGACDLSFIERNIVDLGYMLGI
                     DSWGQGFATEIALALIDAAFFDLRADQVISTVDVNNRASIRVLEKIGMRWEAVFRKHR
                     RAKNRWWDCHLYVLPRAVWETARTQP"
     gene            complement(4657..4824)
                     /locus_tag="VGE92_16950"
     CDS             complement(4657..4824)
                     /locus_tag="VGE92_16950"
                     /inference="COORDINATES: protein motif:HMM:NF022886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2897 family protein"
                     /protein_id="HEY1315575.1"
                     /translation="MFKAIIIIVLVVGAVVGGLLTLRTSGRAGMPSNEVLERAAKRAR
                     DQAAADDKENR"
     gene            complement(4841..5698)
                     /gene="tesB"
                     /locus_tag="VGE92_16955"
     CDS             complement(4841..5698)
                     /gene="tesB"
                     /locus_tag="VGE92_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HEY1315576.1"
                     /translation="MNQLLDDLIKVMTLERLERNLFRGQSRDIGSPQVFGGQVLGQAL
                     VAASSTTEDRVVHSLHAYFLRRGDFNAPIVYEVDRAMDGKHFSSRRVVAIQHGQQIFN
                     MSASFQLTESGLDHQISMPNVPPPESLPDAESYYRKAADQLPAVARRIVEQKRPFEFR
                     PVQPPDFLRREKSEPLKYIWFRAVDTLAPDEALHRCLLAYVSDFHLLDTALKPHGVSM
                     ISSKLVVASIDHAMWFHRSVRVDDWLLYAIDSPSASGARGFARGSVFARDGRLVASTA
                     QEGLMRVTG"
     gene            complement(5708..>6758)
                     /locus_tag="VGE92_16960"
     CDS             complement(5708..>6758)
                     /locus_tag="VGE92_16960"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY1315577.1"
                     /translation="AAAQAAPTPVETVIIGAGPVGLFQIFELGLLGINAHIIDSLPQA
                     GGQCTELYPDKPIYDIPALPICGAQELIDRLLAQAKPFHATFHLGQEVSEVRPHDGKF
                     LVGTSAGTRLLAGAVVIAAGVGSFQPRRLSLPGVEEFEGKSVHYRVKSAADFHGRDLL
                     ICGGGDSALDWTVALADKAHSLTLLHRRAEFRAAPATVARMRELVSAGKMQLLEGNAV
                     GLRKQNDSLVGLDVHTADGRDLQVSADRVLAFYGLHPKLGPIAEWGMELEKRALKVDT
                     ERFQTSIPGIFAIGDINTYPGKKKLILSGFHEAALAAFAIQHHLFPEKRQFLQYTTTS
                     PIMQKRLGVAESDAH"
BASE COUNT         1217 a         2120 c         2118 g         1303 t
ORIGIN      
        1 aattcgcggc ggttcgcgcg aatatgtcaa gcaactcacg gcgccatacg ccaagggttt
       61 actgctgaac acgccggtcg catcgctgca gcgcctgccc acccggatcg tggtgcgatt
      121 gaagaacggc agcgtggaga atttcgatca ggtgttcatc gcctgtcaca gcgaccaggc
      181 gctgaaactg ctgtccgatg cgtcgcacca ggaacgcgaa atcttgggcg caatccctta
      241 ccaggcgaac gaagcattgc tgcacacgga ttcttcgctg atgccgcgcc gctcgcttgc
      301 gtgggcggcg tggaattatc atctgccgat cgaacagttc ggccgggtca cggtcactta
      361 caatatgaac atcctgcaat cgctggatgc gccggcgcag ttcctgctgt cgttgaatcg
      421 cggcgacgcg gtagacccta acaaggtgct gggccgctac gtttatcatc atccggtgta
      481 cacggcggcg gcggtggcgg cgcaagggcg gcggcacgag atcaacgggg tccgccgcac
      541 ttatttctgc ggcgcctatt ggggatatgg ttttcacgaa gatggcgtaa acagtgcgct
      601 cgcttcgctc gaggaatttc aacgtcgcca agctcatgaa cagccgaatt tacaaagggt
      661 gggttgagca tcggcgcttc gcgccgcgct gcaaccgctt tcgttacccg ctgttcatgc
      721 tgtacctcga cttggcggaa ttaccgcggc ttttcgctcg cacgccgttg tggtccgcga
      781 cgcgcccggc tctcgcctgg ttcaagcgcg aggactatct ggcaccccac aacctgccgc
      841 tcgagcaggc cgtgcgcgac ttggtcgctg cgcgaaccgg gcgccggccg agcgggccca
      901 tccggctgtt gacgcatttg cgatacttcg gctactccat gaatccggtg agcctctatt
      961 attgctttga cgaatcgggc ggcacgctcg agaccatcgt cgcggaaatc accaacacgc
     1021 cgtggcgcga acggcaccag tacgtgctga acgtcgactc tgccgcgagc cgcctcaagc
     1081 gcttcgactt cgacaagggc tttcacgttt cgccgttcat gcccatggac atgagatatc
     1141 ggtggtcctt cagcgagccc gggcaacggc tgttcgtcaa tatgcgcaat ttccagggcg
     1201 ccgcccacgt gttcgatgcg actttgagcc tgcgcggcga accggtcacg cgcggcgcgc
     1261 tgctcggcgc cctgttctcg tacccattgc tgagcctgcg ggtcattacc gcgattcatt
     1321 ggcaggcgct gcgcttgtgg ttgaagcgaa cccccttcta cgggcatcct cgtaccttag
     1381 gccgctgaag atcgcccgtg aagcacagca tcattccgct tgaaatcgcc gccaaaccgg
     1441 agcgcggatc cgccttcagc aaactggcaa agggcctgtt gctcagccaa ttgcgcaaga
     1501 tacgcgacgg ccggttgcgc ttgatcgatg ccgatcatgc ggaaaccttc ggcgaaatta
     1561 ccgcggctgg gcccttcgac gtgacgatcc gcgtgcgcca tccgcggttt tataccgacg
     1621 tcgtgctggc cggcacgttg ggctcgggcg aagcctatat gaacggctac tggcagtgcg
     1681 atgatctgac ggccctggtg cgcttgatgg tgatcaaccg ccatttgatg aatgacgtcg
     1741 actctggatg gtcgcgcttg agcgcgccgc tgctgagatt tgcacactgg ctcaatcgca
     1801 atgacaagag cggcagccgc cgcaacatcg cggcgcacta cgacctggga aaccacttct
     1861 tttcgttgtt tctcgatgaa accatggctt attcgagcgc catatttgca cgtgcggatg
     1921 ccacgcttct cgaggcatcg accgcgaaat ttgacgccgc ctgcagcaag ctcgacctga
     1981 agccggatca gcatctactg gaaatcggca ccggctgggg cggcctcgcg attcatgcgg
     2041 ccaagaacta cggttgccgc gtcacgacca ctacgatttc gcgcgaacag ttcgagttcg
     2101 ccaaggaaag cgtcgcacgc gccggcttga gcgagcgcat aacgctgctg ctcgaggact
     2161 atcgcgacct caagggccaa tacgacgccc tggtttccat cgagatgatc gaggctgtcg
     2221 gcgacaggta cctcgacacc tactttcgac gctgcagcgg cttgttgcgt gcgagcggcg
     2281 cgatgttact gcagtcgatc acgattcgcg atcagttcta cgcgcaggcc cggcgctcgg
     2341 tcgattttat caagcgcttt attttcccgg gcagctgtat tccttccatt caggtgttgg
     2401 tggcctcggt ggcgcgcgcc accgatctga agctgtttca catggaggat atcgggcctc
     2461 actatgcacg caccctgcaa gcttggcgcg agcgattcct ggccaggcaa gccgaggtgc
     2521 gcgcgttggg ttatcctgaa tccttcgtgc gcatgtggga gttttatctg tgctattgcg
     2581 aaggggggtt cgaagagcgg cagctgggcg acgtgcagat gctcttgacc aagccgcgct
     2641 cgcggcgcgc ggcgattgcc tgcgttaatg ccgcgggcgc ctgacgcaag aaccctagac
     2701 gagctcaagg ccgtcgtcgg cgcggggttc tggctcgaag cggcggaggc ggtggcgccc
     2761 ttcgtcaacg actttcgccg cctctatcgt ggcgccacgc cgctggtgct ggcgccgcac
     2821 gataccggcg aagttgcgca gatactgcgg atttgcaatc gcgaatccgt ggccgtggta
     2881 ccccacggcg gcaacaccgg ctactgcggc ggtgcgacgc ccgacgcgag cggcaccgag
     2941 atcgtgctgt ccctgcgcag gttgaagcgc gtccgccaga tcgatgcagt caactattcc
     3001 atggtcatcg aggcgggatg cacgctggcc gaagcgcaag cggccgccgc cgatgtcgac
     3061 cggctgttcc cattgagtct gggctcggaa ggaaccgcgc agatcggcgg aaatctctcg
     3121 accaacgccg gcggcaccgc ggtgctgcgc tacgggatga tgcgggatct cgtgctcggc
     3181 cttgaagtcg tgctggcgga tggcagcgtc ctcgagggtc ttaagagcct gcgcaaggac
     3241 aataccggct acgacgtgaa atcgctgttc gtgggcgcgg aaggcacgct cggcgtgatc
     3301 acagcggcct gcctcaagct ctttccgcgc ccggctgaca cggcgacggc gctggtcggc
     3361 atcgactccc cgccacgcgc cttggatctg cttgccggcc tacgcacggc gggcggcgac
     3421 caggtgacct cgttcgaact gataccgcac cgcgccgtgg agctcaccgt caagcatgtc
     3481 ccgggcgtgg ccgacccctc gagccagaat gcgccctggt atttgctcgt cgaactctcc
     3541 tcgccgaatc cgcggcacga gttgtcggcc ctgctgaccg cagcgctgga ggatgcggtg
     3601 ctgcgcggca cgatccgcga cgccatgctc gcgacctcgc ttgcacaagc gcaggccatg
     3661 tggaagctgc gcgaatcggt gcccgaagcg cagcgccgcc atggcgcaag tctcaaacac
     3721 gatgtgtcgg tgccggtgtc ggcgatcccg gccttgattg aagaaggttc cctgctggtg
     3781 cgccgtctgg cacccgaggg cgaagtgatc gcctacggac acgccggcga cggcaacctg
     3841 catttcaatg tgagccaacg gcccgaatgc gatgtccaaa gcttcctggc gcgtggaccc
     3901 gctctcgaac tggcgatctt cgacttggtc gagcgcttgg gcggcagcat cagtgccgaa
     3961 catggcatcg gccggctcaa agccgccgag ttcgcgcgcc gcgccgatcc ggtggaactc
     4021 gccgtgatgc acgccatgaa gcacgcgctc gatcccaacg gcatcatgaa tccgggcaag
     4081 gtgctgtgcg cacgctgatt cttcccgtgt gcaccgcgcg cctgcagttg cgtgagttcg
     4141 ttcgctccga tttcgatgcc gtgtttgcct attcgtccga cccgcgcgtc acccgctacc
     4201 tgtttttcgg cccgcgcgat gcggacggca ccagcgacta tctcgacggg ttgctcgcct
     4261 cgcagcacga gcggccgcgc acccgattcg agctggcggt cgaggaaacc gcttcgggcc
     4321 gcgtcatcgg cgcctgcgac ctgtcgttca tcgagcgcaa catcgtcgat ttgggataca
     4381 tgctggggat cgactcttgg ggccaaggat ttgcgactga gatcgcgctg gcgctgatcg
     4441 acgccgcatt tttcgatctg cgcgccgacc aggtgatctc gacggtggat gtcaacaacc
     4501 gcgcttcgat tcgcgtgctg gagaaaatcg gcatgcgctg ggaagccgtg tttcgcaaac
     4561 atcgccgcgc caagaatcgc tggtgggact gccacctgta cgtgttgccg cgcgcggttt
     4621 gggagaccgc acgcacccag ccttgaggcg ctggatttag cggttttcct tgtcgtccgc
     4681 cgccgcctga tcgcgggcgc gtttcgcggc ccgctcgagc acctcattgc tcggcatgcc
     4741 ggcccgtccg ctggtgcgca aggtcagaag gccgccgacc acggcaccga ccacgagaac
     4801 gatgatgatg atggctttga acatcgcgaa tctccccgct ctagccggta acccgcatca
     4861 atccttcctg ggcggtgctc gccaccaatc tgccgtcgcg cgcgaacacg ctgccgcgcg
     4921 caaagccgcg cgcgcccgag gcgcttgggc tgtcgatggc atacagcagc caatcgtcga
     4981 cgcggacgct gcgatgaaac cacatcgcgt gatcgatgct ggcaaccacc agcttcgaag
     5041 agatcatcga gacaccgtgc ggtttcaatg cggtgtcgag caaatgaaaa tcggacacat
     5101 aggcgagcag gcagcgatgc aacgcttcgt cgggcgccag cgtgtcgacc gcgcgaaacc
     5161 agatgtattt gagcggctcg gatttttccc gccgcaggaa gtcgggcggc tgtaccggtc
     5221 gaaattcgaa cggacgcttt tgctcgacga tccgccgcgc gaccgcaggc agctggtccg
     5281 ccgccttgcg ataatatgat tccgcgtcgg gcaatgactc gggcggcggc acgttcggca
     5341 tcgagatctg gtgatcgaga ccggactcgg tgagctggaa cgaggccgac atattgaata
     5401 tctgctggcc gtgctgaatg gctacgaccc gccgcgagga gaaatgcttg ccgtccatcg
     5461 cccggtcgac ctcatagacg atgggcgcat tgaagtcacc gcggcgcagg aaatacgcat
     5521 gcagcgagtg caccacgcga tcctcggtgg ttgagcttgc cgccaccaag gcctgtccca
     5581 gaacttgccc gccgaatacc tgcggactgc cgatgtcgcg gctttgcccg cgaaacagat
     5641 ttctctcgag ccgctcgaga gtcatcactt tgatcagatc gtcgagcagc tggttcattg
     5701 ccgagcctca atgcgcgtcg ctttcagcca cgccgagacg cttctgcatg atcggactgg
     5761 tggtggtgta ctgcaggaat tggcgtttct ccgggaacag gtgatgctgg atggcgaagg
     5821 ccgcgagcgc tgcctcgtga aagccgctca aaatgagctt tttcttgccg ggataggtgt
     5881 tgatatcgcc gatcgcgaag atacccggaa tgctggtctg gaagcgctcg gtgtcgacct
     5941 tgagcgcgcg tttttccaat tccatgcccc actcggcaat gggcccaagc ttcggatgca
     6001 gtccgtagaa cgcgagcacc cgatcagcgg acacttgcaa gtcgcgcccg tccgccgtgt
     6061 gcacgtcgag acccaccagc gaatcgtttt gcttgcgcaa gccgacggcg ttgccctcga
     6121 gcaactgcat cttgcccgcg ctcaccagtt cgcgcatgcg cgcgacggtg gcgggggcgg
     6181 cgcgaaactc ggcgcgacga tgcagcaggg tgagcgaatg cgctttgtcg gcgagcgcca
     6241 cggtccagtc caaggccgag tccccaccgc cgcagatcag aaggtcgcga ccgtgaaaat
     6301 ccgcggccga tttgacccga taatgcacgc ttttgccctc gaactcctcg acgccgggga
     6361 gactcaaacg gcgcggctga aaggaaccga ccccggccgc gatgaccacg gcgccggcca
     6421 agagccgcgt accggcggaa gtgccgacga gaaacttgcc atcgtgcggc cgcacctcgc
     6481 tgacttcctg accgagatgg aaggtggcgt ggaacggttt tgcctgcgcc aacagccggt
     6541 cgatcaattc ctgcgcgccg cagatcggca aagcggggat atcatatatc ggcttgtccg
     6601 gatacagttc ggtgcactgg ccgcccgctt gcggcaacga atcaatgatg tgcgcgttga
     6661 tcccgagaag tccgagttcg aagatctgaa acagtccgac gggacctgcg ccgataatca
     6721 cggtctcgac cggggtggga gcggcttgtg ccgcagcg
//
